LOCUS       DAFNBJ010000001       129694 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000001 DAFNBJ010000000
VERSION     DAFNBJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 129694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 129694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..129694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.001"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            18..185
                     /locus_tag="L2X49_000001"
     CDS             18..185
                     /locus_tag="L2X49_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBN4795156.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            429..1817
                     /gene="frlA"
                     /locus_tag="L2X49_000002"
     CDS             429..1817
                     /gene="frlA"
                     /locus_tag="L2X49_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HBN4795157.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            1838..2860
                     /gene="frlB"
                     /locus_tag="L2X49_000003"
     CDS             1838..2860
                     /gene="frlB"
                     /locus_tag="L2X49_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBN4795158.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2910..3740
                     /gene="frlC"
                     /locus_tag="L2X49_000004"
     CDS             2910..3740
                     /gene="frlC"
                     /locus_tag="L2X49_000004"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HBN4795159.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYDGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3737..4522
                     /gene="frlD"
                     /locus_tag="L2X49_000005"
     CDS             3737..4522
                     /gene="frlD"
                     /locus_tag="L2X49_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBN4795160.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4622..5353
                     /gene="phoB"
                     /locus_tag="L2X49_000006"
     CDS             4622..5353
                     /gene="phoB"
                     /locus_tag="L2X49_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBN4795161.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5505..6590)
                     /locus_tag="L2X49_000007"
     CDS             complement(5505..6590)
                     /locus_tag="L2X49_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBN4795162.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6602..7906)
                     /locus_tag="L2X49_000008"
     CDS             complement(6602..7906)
                     /locus_tag="L2X49_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBN4795163.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7918..8271)
                     /locus_tag="L2X49_000009"
     CDS             complement(7918..8271)
                     /locus_tag="L2X49_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBN4795164.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8282..9160)
                     /locus_tag="L2X49_000010"
     CDS             complement(8282..9160)
                     /locus_tag="L2X49_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBN4795165.1"
                     /translation="MSFDPTGYTLVHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVDHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(9157..10383)
                     /locus_tag="L2X49_000011"
     CDS             complement(9157..10383)
                     /locus_tag="L2X49_000011"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBN4795166.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10383..11546)
                     /locus_tag="L2X49_000012"
     CDS             complement(10383..11546)
                     /locus_tag="L2X49_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBN4795167.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(11630..11992)
                     /locus_tag="L2X49_000013"
     CDS             complement(11630..11992)
                     /locus_tag="L2X49_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBN4795168.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AITHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(12009..12914)
                     /locus_tag="L2X49_000014"
     CDS             complement(12009..12914)
                     /locus_tag="L2X49_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795169.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13183..14187)
                     /gene="trpS"
                     /locus_tag="L2X49_000015"
     CDS             complement(13183..14187)
                     /gene="trpS"
                     /locus_tag="L2X49_000015"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBN4795170.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14180..14938)
                     /gene="gph"
                     /locus_tag="L2X49_000016"
     CDS             complement(14180..14938)
                     /gene="gph"
                     /locus_tag="L2X49_000016"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBN4795171.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14931..15608)
                     /gene="rpe"
                     /locus_tag="L2X49_000017"
     CDS             complement(14931..15608)
                     /gene="rpe"
                     /locus_tag="L2X49_000017"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBN4795172.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15626..16462)
                     /gene="dam"
                     /locus_tag="L2X49_000018"
     CDS             complement(15626..16462)
                     /gene="dam"
                     /locus_tag="L2X49_000018"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBN4795173.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16569..17855)
                     /gene="damX"
                     /locus_tag="L2X49_000019"
     CDS             complement(16569..17855)
                     /gene="damX"
                     /locus_tag="L2X49_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBN4795174.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17947..19035)
                     /gene="aroB"
                     /locus_tag="L2X49_000020"
     CDS             complement(17947..19035)
                     /gene="aroB"
                     /locus_tag="L2X49_000020"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBN4795175.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEIIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19092..19613)
                     /gene="aroK"
                     /locus_tag="L2X49_000021"
     CDS             complement(19092..19613)
                     /gene="aroK"
                     /locus_tag="L2X49_000021"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBN4795176.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(20014..21252)
                     /gene="hofQ"
                     /locus_tag="L2X49_000022"
     CDS             complement(20014..21252)
                     /gene="hofQ"
                     /locus_tag="L2X49_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBN4795177.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21164..21568)
                     /gene="hofP"
                     /locus_tag="L2X49_000023"
     CDS             complement(21164..21568)
                     /gene="hofP"
                     /locus_tag="L2X49_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBN4795178.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21558..21998)
                     /gene="hofO"
                     /locus_tag="L2X49_000024"
     CDS             complement(21558..21998)
                     /gene="hofO"
                     /locus_tag="L2X49_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBN4795179.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21982..22521)
                     /gene="hofN"
                     /locus_tag="L2X49_000025"
     CDS             complement(21982..22521)
                     /gene="hofN"
                     /locus_tag="L2X49_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBN4795180.1"
                     /translation="MNLPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22521..23300)
                     /gene="hofM"
                     /locus_tag="L2X49_000026"
     CDS             complement(22521..23300)
                     /gene="hofM"
                     /locus_tag="L2X49_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBN4795181.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGGFAIALGLALGKAY"
     gene            23420..25972
                     /gene="mrcA"
                     /locus_tag="L2X49_000027"
     CDS             23420..25972
                     /gene="mrcA"
                     /locus_tag="L2X49_000027"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBN4795182.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26137..26697)
                     /gene="nudE"
                     /locus_tag="L2X49_000028"
     CDS             complement(26137..26697)
                     /gene="nudE"
                     /locus_tag="L2X49_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBN4795183.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            27017..29152
                     /gene="igaA"
                     /locus_tag="L2X49_000029"
     CDS             27017..29152
                     /gene="igaA"
                     /locus_tag="L2X49_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBN4795184.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTNIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29217..29885
                     /gene="yrfG"
                     /locus_tag="L2X49_000030"
     CDS             29217..29885
                     /gene="yrfG"
                     /locus_tag="L2X49_000030"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBN4795185.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29896..30297
                     /gene="hslR"
                     /locus_tag="L2X49_000031"
     CDS             29896..30297
                     /gene="hslR"
                     /locus_tag="L2X49_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBN4795186.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30322..31200
                     /gene="hslO"
                     /locus_tag="L2X49_000032"
     CDS             30322..31200
                     /gene="hslO"
                     /locus_tag="L2X49_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBN4795187.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31335..33059)
                     /locus_tag="L2X49_000033"
     CDS             complement(31335..33059)
                     /locus_tag="L2X49_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBN4795188.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            33438..35060
                     /gene="pckA"
                     /locus_tag="L2X49_000034"
     CDS             33438..35060
                     /gene="pckA"
                     /locus_tag="L2X49_000034"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBN4795189.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35136..36488)
                     /gene="envZ"
                     /locus_tag="L2X49_000035"
     CDS             complement(35136..36488)
                     /gene="envZ"
                     /locus_tag="L2X49_000035"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBN4795190.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEMRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36485..37204)
                     /gene="ompR"
                     /locus_tag="L2X49_000036"
     CDS             complement(36485..37204)
                     /gene="ompR"
                     /locus_tag="L2X49_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBN4795191.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37432..37908
                     /gene="greB"
                     /locus_tag="L2X49_000037"
     CDS             37432..37908
                     /gene="greB"
                     /locus_tag="L2X49_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBN4795192.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            38005..40326
                     /locus_tag="L2X49_000038"
     CDS             38005..40326
                     /locus_tag="L2X49_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBN4795193.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40764..40991
                     /gene="feoA"
                     /locus_tag="L2X49_000039"
     CDS             40764..40991
                     /gene="feoA"
                     /locus_tag="L2X49_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBN4795194.1"
                     /translation="MQYTPDTAWKITGFSHEISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            41008..43329
                     /gene="feoB"
                     /locus_tag="L2X49_000040"
     CDS             41008..43329
                     /gene="feoB"
                     /locus_tag="L2X49_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBN4795195.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43329..43565
                     /gene="feoC"
                     /locus_tag="L2X49_000041"
     CDS             43329..43565
                     /gene="feoC"
                     /locus_tag="L2X49_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBN4795196.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43768..44646
                     /gene="rpnA"
                     /locus_tag="L2X49_000042"
     CDS             43768..44646
                     /gene="rpnA"
                     /locus_tag="L2X49_000042"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBN4795197.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(44675..45445)
                     /gene="bioH"
                     /locus_tag="L2X49_000043"
     CDS             complement(44675..45445)
                     /gene="bioH"
                     /locus_tag="L2X49_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBN4795198.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45483..46166
                     /gene="gntX"
                     /locus_tag="L2X49_000044"
     CDS             45483..46166
                     /gene="gntX"
                     /locus_tag="L2X49_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBN4795199.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46225..46800
                     /gene="nfuA"
                     /locus_tag="L2X49_000045"
     CDS             46225..46800
                     /gene="nfuA"
                     /locus_tag="L2X49_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBN4795200.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47161..48477
                     /gene="gntT"
                     /locus_tag="L2X49_000046"
     CDS             47161..48477
                     /gene="gntT"
                     /locus_tag="L2X49_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBN4795201.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48522..50606)
                     /gene="malQ"
                     /locus_tag="L2X49_000047"
     CDS             complement(48522..50606)
                     /gene="malQ"
                     /locus_tag="L2X49_000047"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBN4795202.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50616..53009)
                     /gene="malP"
                     /locus_tag="L2X49_000048"
     CDS             complement(50616..53009)
                     /gene="malP"
                     /locus_tag="L2X49_000048"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBN4795203.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            53620..56325
                     /gene="malT"
                     /locus_tag="L2X49_000049"
     CDS             53620..56325
                     /gene="malT"
                     /locus_tag="L2X49_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBN4795204.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(56368..57384)
                     /gene="rtcA"
                     /locus_tag="L2X49_000050"
     CDS             complement(56368..57384)
                     /gene="rtcA"
                     /locus_tag="L2X49_000050"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBN4795205.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(57388..58614)
                     /gene="rtcB"
                     /locus_tag="L2X49_000051"
     CDS             complement(57388..58614)
                     /gene="rtcB"
                     /locus_tag="L2X49_000051"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBN4795206.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQQQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            58803..60401
                     /gene="rtcR"
                     /locus_tag="L2X49_000052"
     CDS             58803..60401
                     /gene="rtcR"
                     /locus_tag="L2X49_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBN4795207.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(60383..61141)
                     /locus_tag="L2X49_000053"
     CDS             complement(60383..61141)
                     /locus_tag="L2X49_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBN4795208.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(61158..61988)
                     /gene="glpG"
                     /locus_tag="L2X49_000054"
     CDS             complement(61158..61988)
                     /gene="glpG"
                     /locus_tag="L2X49_000054"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBN4795209.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(62033..62359)
                     /gene="glpE"
                     /locus_tag="L2X49_000055"
     CDS             complement(62033..62359)
                     /gene="glpE"
                     /locus_tag="L2X49_000055"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBN4795210.1"
                     /translation="MDQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            62549..64054
                     /gene="glpD"
                     /locus_tag="L2X49_000056"
     CDS             62549..64054
                     /gene="glpD"
                     /locus_tag="L2X49_000056"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBN4795211.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(64108..64713)
                     /locus_tag="L2X49_000057"
     CDS             complement(64108..64713)
                     /locus_tag="L2X49_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795212.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(64716..65483)
                     /locus_tag="L2X49_000058"
     CDS             complement(64716..65483)
                     /locus_tag="L2X49_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795213.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(65486..66196)
                     /locus_tag="L2X49_000059"
     CDS             complement(65486..66196)
                     /locus_tag="L2X49_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795214.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(66211..67719)
                     /locus_tag="L2X49_000060"
     CDS             complement(66211..67719)
                     /locus_tag="L2X49_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBN4795215.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(67844..70291)
                     /gene="glgP"
                     /locus_tag="L2X49_000061"
     CDS             complement(67844..70291)
                     /gene="glgP"
                     /locus_tag="L2X49_000061"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBN4795216.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(70310..71743)
                     /gene="glgA"
                     /locus_tag="L2X49_000062"
     CDS             complement(70310..71743)
                     /gene="glgA"
                     /locus_tag="L2X49_000062"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBN4795217.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(71743..73038)
                     /gene="glgC"
                     /locus_tag="L2X49_000063"
     CDS             complement(71743..73038)
                     /gene="glgC"
                     /locus_tag="L2X49_000063"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBN4795218.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(73056..75029)
                     /gene="glgX"
                     /locus_tag="L2X49_000064"
     CDS             complement(73056..75029)
                     /gene="glgX"
                     /locus_tag="L2X49_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBN4795219.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARCFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(75026..77212)
                     /gene="glgB"
                     /locus_tag="L2X49_000065"
     CDS             complement(75026..77212)
                     /gene="glgB"
                     /locus_tag="L2X49_000065"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBN4795220.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(77485..78588)
                     /gene="asd"
                     /locus_tag="L2X49_000066"
     CDS             complement(77485..78588)
                     /gene="asd"
                     /locus_tag="L2X49_000066"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBN4795221.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            78781..79374
                     /gene="yhgN"
                     /locus_tag="L2X49_000067"
     CDS             78781..79374
                     /gene="yhgN"
                     /locus_tag="L2X49_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBN4795222.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(79431..80771)
                     /gene="gntU"
                     /locus_tag="L2X49_000068"
     CDS             complement(79431..80771)
                     /gene="gntU"
                     /locus_tag="L2X49_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBN4795223.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     FAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(80775..81302)
                     /gene="gntK"
                     /locus_tag="L2X49_000069"
     CDS             complement(80775..81302)
                     /gene="gntK"
                     /locus_tag="L2X49_000069"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBN4795224.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(81441..82436)
                     /gene="gntR"
                     /locus_tag="L2X49_000070"
     CDS             complement(81441..82436)
                     /gene="gntR"
                     /locus_tag="L2X49_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBN4795225.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEIEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(82660..83355)
                     /gene="yhhW"
                     /locus_tag="L2X49_000071"
     CDS             complement(82660..83355)
                     /gene="yhhW"
                     /locus_tag="L2X49_000071"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBN4795226.1"
                     /translation="MIYFRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(83478..84515)
                     /locus_tag="L2X49_000072"
     CDS             complement(83478..84515)
                     /locus_tag="L2X49_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBN4795227.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHLTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            84848..85336
                     /gene="yhhY"
                     /locus_tag="L2X49_000073"
     CDS             84848..85336
                     /gene="yhhY"
                     /locus_tag="L2X49_000073"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBN4795228.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LAAHPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            85446..85730
                     /locus_tag="L2X49_000074"
     CDS             85446..85730
                     /locus_tag="L2X49_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HBN4795229.1"
                     /translation="MMIYQEAFAKANHYLDDADLPVVITLHGRFSQGWYFCFESQEFL
                     ESGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLHDYEIKKATFGLP"
     gene            complement(85768..87501)
                     /gene="ggt"
                     /locus_tag="L2X49_000075"
     CDS             complement(85768..87501)
                     /gene="ggt"
                     /locus_tag="L2X49_000075"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBN4795230.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRTKD
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            87622..88062
                     /locus_tag="L2X49_000076"
     CDS             87622..88062
                     /locus_tag="L2X49_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBN4795231.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(88049..88792)
                     /gene="ugpQ"
                     /locus_tag="L2X49_000077"
     CDS             complement(88049..88792)
                     /gene="ugpQ"
                     /locus_tag="L2X49_000077"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBN4795232.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTMRLGCVSLHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(88789..89859)
                     /gene="ugpC"
                     /locus_tag="L2X49_000078"
     CDS             complement(88789..89859)
                     /gene="ugpC"
                     /locus_tag="L2X49_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HBN4795233.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(89861..90706)
                     /gene="ugpE"
                     /locus_tag="L2X49_000079"
     CDS             complement(89861..90706)
                     /gene="ugpE"
                     /locus_tag="L2X49_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBN4795234.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(90703..91590)
                     /gene="ugpA"
                     /locus_tag="L2X49_000080"
     CDS             complement(90703..91590)
                     /gene="ugpA"
                     /locus_tag="L2X49_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBN4795235.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(91688..93004)
                     /gene="ugpB"
                     /locus_tag="L2X49_000081"
     CDS             complement(91688..93004)
                     /gene="ugpB"
                     /locus_tag="L2X49_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBN4795236.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(93401..94114)
                     /gene="livF"
                     /locus_tag="L2X49_000082"
     CDS             complement(93401..94114)
                     /gene="livF"
                     /locus_tag="L2X49_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBN4795237.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(94116..94883)
                     /gene="livG"
                     /locus_tag="L2X49_000083"
     CDS             complement(94116..94883)
                     /gene="livG"
                     /locus_tag="L2X49_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBN4795238.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(94880..96157)
                     /gene="livM"
                     /locus_tag="L2X49_000084"
     CDS             complement(94880..96157)
                     /gene="livM"
                     /locus_tag="L2X49_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBN4795239.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(96154..97080)
                     /gene="livH"
                     /locus_tag="L2X49_000085"
     CDS             complement(96154..97080)
                     /gene="livH"
                     /locus_tag="L2X49_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBN4795240.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTVFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(97128..98237)
                     /gene="livK"
                     /locus_tag="L2X49_000086"
     CDS             complement(97128..98237)
                     /gene="livK"
                     /locus_tag="L2X49_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBN4795241.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            98661..99044
                     /gene="panM"
                     /locus_tag="L2X49_000087"
     CDS             98661..99044
                     /gene="panM"
                     /locus_tag="L2X49_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBN4795242.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLCVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(99041..99568)
                     /locus_tag="L2X49_000088"
     CDS             complement(99041..99568)
                     /locus_tag="L2X49_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN4795243.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(99575..99841)
                     /locus_tag="L2X49_000089"
     CDS             complement(99575..99841)
                     /locus_tag="L2X49_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBN4795244.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            complement(99991..101094)
                     /gene="livJ"
                     /locus_tag="L2X49_000090"
     CDS             complement(99991..101094)
                     /gene="livJ"
                     /locus_tag="L2X49_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBN4795245.1"
                     /translation="MNMKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSRAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(101366..102220)
                     /gene="rpoH"
                     /locus_tag="L2X49_000091"
     CDS             complement(101366..102220)
                     /gene="rpoH"
                     /locus_tag="L2X49_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBN4795246.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(102465..103523)
                     /gene="ftsX"
                     /locus_tag="L2X49_000092"
     CDS             complement(102465..103523)
                     /gene="ftsX"
                     /locus_tag="L2X49_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBN4795247.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(103516..104184)
                     /gene="ftsE"
                     /locus_tag="L2X49_000093"
     CDS             complement(103516..104184)
                     /gene="ftsE"
                     /locus_tag="L2X49_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBN4795248.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(104187..105680)
                     /gene="ftsY"
                     /locus_tag="L2X49_000094"
     CDS             complement(104187..105680)
                     /gene="ftsY"
                     /locus_tag="L2X49_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBN4795249.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            105830..106426
                     /gene="rsmD"
                     /locus_tag="L2X49_000095"
     CDS             105830..106426
                     /gene="rsmD"
                     /locus_tag="L2X49_000095"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBN4795250.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            106416..106685
                     /locus_tag="L2X49_000096"
     CDS             106416..106685
                     /locus_tag="L2X49_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBN4795251.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(106688..107047)
                     /locus_tag="L2X49_000097"
     CDS             complement(106688..107047)
                     /locus_tag="L2X49_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBN4795252.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            107188..107814
                     /locus_tag="L2X49_000098"
     CDS             107188..107814
                     /locus_tag="L2X49_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBN4795253.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            107888..110086
                     /gene="zntA"
                     /locus_tag="L2X49_000099"
     CDS             107888..110086
                     /gene="zntA"
                     /locus_tag="L2X49_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBN4795254.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(110188..110433)
                     /gene="tusA"
                     /locus_tag="L2X49_000100"
     CDS             complement(110188..110433)
                     /gene="tusA"
                     /locus_tag="L2X49_000100"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBN4795255.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            110654..111319
                     /locus_tag="L2X49_000101"
     CDS             110654..111319
                     /locus_tag="L2X49_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBN4795256.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            111392..111949
                     /gene="dcrB"
                     /locus_tag="L2X49_000102"
     CDS             111392..111949
                     /gene="dcrB"
                     /locus_tag="L2X49_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBN4795257.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVLQ"
     gene            complement(111953..113170)
                     /locus_tag="L2X49_000103"
     CDS             complement(111953..113170)
                     /locus_tag="L2X49_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4795258.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            113302..114351
                     /locus_tag="L2X49_000104"
     CDS             113302..114351
                     /locus_tag="L2X49_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBN4795259.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            114406..114993
                     /gene="acpT"
                     /locus_tag="L2X49_000105"
     CDS             114406..114993
                     /gene="acpT"
                     /locus_tag="L2X49_000105"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBN4795260.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            115104..116678
                     /gene="nikA"
                     /locus_tag="L2X49_000106"
     CDS             115104..116678
                     /gene="nikA"
                     /locus_tag="L2X49_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBN4795261.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            116678..117622
                     /gene="nikB"
                     /locus_tag="L2X49_000107"
     CDS             116678..117622
                     /gene="nikB"
                     /locus_tag="L2X49_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBN4795262.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            117619..118452
                     /gene="nikC"
                     /locus_tag="L2X49_000108"
     CDS             117619..118452
                     /gene="nikC"
                     /locus_tag="L2X49_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBN4795263.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            118452..119216
                     /gene="nikD"
                     /locus_tag="L2X49_000109"
     CDS             118452..119216
                     /gene="nikD"
                     /locus_tag="L2X49_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBN4795264.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            119213..120019
                     /gene="nikE"
                     /locus_tag="L2X49_000110"
     CDS             119213..120019
                     /gene="nikE"
                     /locus_tag="L2X49_000110"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBN4795265.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            120025..120426
                     /gene="nikR"
                     /locus_tag="L2X49_000111"
     CDS             120025..120426
                     /gene="nikR"
                     /locus_tag="L2X49_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBN4795266.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(120546..120905)
                     /locus_tag="L2X49_000112"
     CDS             complement(120546..120905)
                     /locus_tag="L2X49_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBN4795267.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(120902..121177)
                     /locus_tag="L2X49_000113"
                     /pseudo
     CDS             complement(120902..121177)
                     /locus_tag="L2X49_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(121236..122360)
                     /locus_tag="L2X49_000114"
     CDS             complement(121236..122360)
                     /locus_tag="L2X49_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4795268.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(122360..125095)
                     /gene="rbbA"
                     /locus_tag="L2X49_000115"
     CDS             complement(122360..125095)
                     /gene="rbbA"
                     /locus_tag="L2X49_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBN4795269.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(125092..126159)
                     /locus_tag="L2X49_000116"
     CDS             complement(125092..126159)
                     /locus_tag="L2X49_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBN4795270.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(126525..128147)
                     /locus_tag="L2X49_000117"
     CDS             complement(126525..128147)
                     /locus_tag="L2X49_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBN4795271.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGNQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKYYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(128409..>129694)
                     /locus_tag="L2X49_000118"
     CDS             complement(128409..>129694)
                     /locus_tag="L2X49_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBN4795272.1"
                     /translation="TDTFIFTIIPDNNHVLKLSSPITVTVECKGGYYFINSSGDKSDI
                     IYKVDGLSIIARNFFTLLSGNFKPDWRWDVSKETFTKEKFDSYVKPVFSKIDFYKQCG
                     VINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHEEMASSAYKH
                     KNNKVLYDLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLL
                     NSMRNMEGNKDSRINKNVVVLAGNHEINFNGNYTARLANHKLSAGDTYNLIKTLDVCN
                     YDSERQVLTSHHGIIRDEEKKCYCLGALQVPFNQMKNPTDPEELANIFNKKHKEHMDD
                     RFIHLIRSNAIASTPVYDNYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQH
                     QKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPH"
BASE COUNT        30972 a        35501 c        32619 g        30602 t
ORIGIN      
        1 atacataggg aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg
       61 ctacgttaac tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact
      121 acctgctgca cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac
      181 agtaagtctc tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt
      241 tccgagggcg tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt
      301 taaattcaat ttgataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg
      361 atcttcctcc acattacata acatcatatg ttgttatatt catcatgcat tgtcatgtta
      421 ccttttaaat gactgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta
      481 tgggaagcca ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg
      541 ggacaaccgt cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca
      601 cgccgtggct tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt
      661 gcgtctatgc ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga
      721 aaaatgccgg aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg
      781 atgcgccgtc attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc
      841 ctatcgatcc gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc
      901 tacacctgcg ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa
      961 ttatcccgtt cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg
     1021 ccgcccctac caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga
     1081 tctctgccac cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa
     1141 ttaaaaaccc cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg
     1201 tgctctacac cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca
     1261 attctgaaac gccgatttcc gacgccctga cctggatccc cgcactcggc agcaccgctg
     1321 ggatctttgt tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga
     1381 tgtaccagcc gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg
     1441 gccatgtgca tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgctgg
     1501 ggatcttctt catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga
     1561 tgtgtttcaa aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt
     1621 acaaaccgct gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa
     1681 gcctcattct ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg
     1741 tcatcgttat tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc
     1801 tcaacgcttt gtcgtaatgt tatctggaga aaataaaatg ttggatattg ataaaagcac
     1861 cgtggacttt ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga
     1921 cgttccgctg gtgcacgcca tcgtggaaga gatggtgaag cgcgacattg atcgtattta
     1981 tttcgttgcc tgcggctcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg
     2041 cttttccgat cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta
     2101 tcgcctcgac gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt
     2161 aatcaaagcg ctggagctgg gccgggcctg cggcgcactc actgcggcgt tcaccaaacg
     2221 cgcggacagc ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat
     2281 ctgggaaatt cacctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc
     2341 gccgaacgcg gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg
     2401 tcatctggta cgcacctggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg
     2461 gccgatgatt tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat
     2521 tgtaacgctg atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg
     2581 ccatggcccg ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga
     2641 tgaaagtcgc cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt
     2701 gatcgtcatc gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct
     2761 gatgttcgtg ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc
     2821 ggatgaacgc cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa
     2881 cgggccgaat ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc
     2941 agcggctgcc tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg
     3001 aaatttgggg cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac
     3061 aaatcaaggc gctggcgcag acgtatcaga tgccgattat cggctatacg ccagaaacca
     3121 acggctatcc gtataacatg atgctgggcg atgaacatat gcgtcgcgaa agcctcgaca
     3181 tgatcaagct ggcgatggat atggcaaaag agatgaacgc gggttatacg ctgatttccg
     3241 cggcccacgc gggctatctc acgccaccta atgttatctg gggacggctg gcagagaacc
     3301 taagcgaact gtgtgagtac gcagaaaaca tcggcatgga tctgatcctc gaaccgttaa
     3361 cgccgtatga atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg
     3421 tgccttcgcc gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac
     3481 cggtgatgag ttatttcgac aaactgggtg ataaattgcg tcatctgcat attgtcgaca
     3541 gcgacggggc cagtgacaca cattacattc ccggcgaagg caaaatgccg ctgcgggagc
     3601 tgatgcgcga tattatcgac cggggctatg acggttactg cacggtggaa ctggtgacga
     3661 tgtatatgaa cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc
     3721 tgccggagga tgagagatga aaaccctggc gacaatcggc gataactgcg tcgatatcta
     3781 cccgcaactg aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac
     3841 tcgctacggc atacagccgg gatgcattac ctgggtgggt gacgatgact acggcacaaa
     3901 gctgaagcag gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg
     3961 cgttaccgca caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac
     4021 cgaaggcgtg atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta
     4081 tgacattgtg cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc
     4141 tgcgggcaaa cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac
     4201 actggtgccg catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg
     4261 tctgaagatg aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga
     4321 aaacggcagc attgcctggg atggcgcgca gttctggcgt caggctcctg aaccggtgac
     4381 ggttatcgac accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc
     4441 tgcggggatg acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat
     4501 tcagtaccac ggtgcctggt aggtataacg ttggcgtgag catcttcacg ccaacgtgct
     4561 gttacttgcc ggaaaacgac cctataatcc gagtaattca ttctttattt cagggtcgat
     4621 tatgtcagct acggaccgct actctcatca actcctctac gctaccgtcc gccagcgact
     4681 gctggatgat atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga
     4741 gctttgtaca caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt
     4801 ggcagacggc gtactgatcc gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt
     4861 tgaaaacgcc ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaaggc
     4921 gacgaaagag aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct
     4981 gaacatcccc ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga
     5041 gccgctgttt attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat
     5101 ttacgtcgaa ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt
     5161 cagcgacaaa aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa
     5221 atgcgaactg ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa
     5281 accggtgcac gtctccgaac tcttctgccg cgccaatcgc atcaccttaa ctattgataa
     5341 taaaagacat taaccgtagg ccggataaga tgcgccagca tcgcatccgg cgatgctggc
     5401 gcgttgaatt ttacatcccg tacgttcctc tcaccctaac cctctcccca aaggggcgag
     5461 gggaccgttc agtacggaat ttagcttgac ggtgctatcc agcattaaat actggcaata
     5521 ctctcacgca aaatccgcag caccgtctct tcaccgctgc gattcgggtt aatgcgaatc
     5581 gcacaatgtt ctgattgtgg attcgcctgg cgaaacgttc cggaaaggcg ataaaagagc
     5641 ggcgggattt catatttcga ctctgcaccc accgggtaag gcaaggcacc gcgcttttgc
     5701 gcctcttcca gcactctggc ggcaatcggc tgatgaaact cgacaatcaa caccttcgac
     5761 tgcgcattag caatcaccgc gcttttcact tccggcaccg caccaccgtt aagcaaagcc
     5821 agcaaccgtt cagatacccc tgcctgcacc gcgtgcatca ctggcgcaaa caccagacca
     5881 cgcaagactt ccagcgcctg tgcgccctgg atctggctac cgccggagta aagcgtggcg
     5941 cgaatacggt tgataacatc agcatcgccg accactgcac caacaccctc tggcccaaat
     6001 agcttgaagc aggaaaatgt cgagacattc gcgccgcatt cacagccgat tcgcgccacc
     6061 ttcatcaccg catagttgtc atcggttaac gctggaacac ctgccgcgcg caacgttgcc
     6121 agcacatctg ccagcacgta gctgtcctgc ggctgctggc gcgtatgctg caccagcgcc
     6181 gcatccggtt gttgctcgtc gacgacctgc ttcagtgccg acaggtcatt gaaatcaaca
     6241 gtaataagcg tcagccccat ctgctcaata ataacccgtg tcgtcgggta aacaggcgcg
     6301 tcatgcacca gaagacgctg ccccggtttg agcaaagccg ccagcccggc gcgaatcgcg
     6361 ccagtccccg cgccctgcac cagcgccgca gcctgtgcgt gaaatgcatc agccagcacc
     6421 tgttccacac gctgggtaac gcgcggttga ttcagtcctg gcgttaagcc taaatcaccg
     6481 ccagtaagaa actcgctgcc ggggaaatgg cgacaaatgc tatccaccag cgcaaactgc
     6541 ttttgctgcg cctcaataat cgtcaggctt tgcagaggaa acgtcttcat aggtgccttc
     6601 cttaagccgg aacgaacagg ccaagccagt aaagaacatt cagcagaata ccggtgatca
     6661 ttactgccac gaccggtgcc gccattttct gtaccggacg gcccagggat tcgttgagga
     6721 agtaaatggc aaccgcgata gagaatccgg tataacccgc catcttaatt gctgcgaaaa
     6781 tcgaaccgac cagcagcgcc acttccatca gcatattcat ggcgttacgg atgttatccg
     6841 acgcattacg caccgacggg tagcgtccca gccatttgcc gatcgaacga agcagcaaga
     6901 cttccgccga aataaccact gcgcctaata ccgctgcaac catcggattc ggcgagagat
     6961 agcccaccgc ataaacaaag gtaaagcccg caactgcata cacacccgtt gctaaggcgg
     7021 tggtggcaat caacggtaca aaacccagcc cgcgcataaa ttctgccagt gccgcctggt
     7081 tgatcagcgt ttgcgattgt tccggcgtta cgcctgcgga gtacgctttc tccagagtga
     7141 agatcgacac ttcactgccg gcgaaaatct tcatgctggc aacggcggca atcaatgctc
     7201 ccacgatggc gatatagggt aagtttttga taatccgcga cgtgcgttct tcaaacaccg
     7261 aaagcccgct ggcatcaata tcattttcat cacgatgacg caggtcgtga gttatcgcga
     7321 tccccagcag catcaccatg ccaataaaga tttcgatgga ttcagggtta agatgtggga
     7381 aatagcgcac gactaccaca cgggtcatca gaacaaccac ggcggcgacc agactttgct
     7441 tccagccaaa ctggtagaaa atcgccacca gcgggaacag tgcaaaagca gaaaccaccg
     7501 gcgagcttaa ttccccgagg ctacctaata catccaccgg cagcgcggtc agcagctggt
     7561 ttactggcaa caggcaagta aggatcaata cgccccagat agcgccaaga ccaaacgcca
     7621 tcaggctgtt tatcgccagt acgccgagga tatcggtagg aagaaagagt aaccatgcgt
     7681 tgagcagtcc ggttttcagg gtaaaagaga tccccaccga ggccacaaaa ccaatgctca
     7741 gaccaaaagc gatactcccc gcctcgcgac ggttcatata gccttcaatc agttgcggca
     7801 ggatcgggcg gatgccgtca tgaaaaacag ccgccgagcg atgcgccaga agcgatgtca
     7861 tacccgtcag gcacgccacc acgataatct gaatatacag atccatagtc gtgcctttta
     7921 tttcagatgg ttaatcagca tcggaatcgc gtgttcgacg tgctcaacgg aaaggccaaa
     7981 cgccactttt ccttcagcga tcattttggc gatatgctcg tctttcgctt taatgcctgg
     8041 tttggcaatg gtgcaacttt tgttatagcc aatcaccgcg atggcaatcg acaatgccgc
     8101 gcccgcaccg gtattacacg caccgatgta gtaatccagt tgcccggatt tcactttcat
     8161 tgccgcttcc atgtcgttgt gaatgaaaac ttcaaaactg ccaggagccg tcgcttcaat
     8221 ggttttttta atctgctcac gctgtaagcc tgcaacgcca atctttttca tgagtctgtc
     8281 cttattggaa aaattgagag ggagtttcac gtaacatcac atccacatcg gcctgactga
     8341 atcctgactg gcgcaattgc ggaataaagg tggtcaataa atagtcatag ccataaccac
     8401 cattggcttt taaatgggag cggcgcgtaa tatccatcga cagcatgacg cggttcagca
     8461 acccacggtc acgtagcgcg tggagcatcg caatgcgctt ttcgtccggg tagtaactgt
     8521 tcttaccaat ggtgtcgaac tgcacgtacg cgccgagatc gatcatcttc aaaatgttgt
     8581 cgaggttgtc tttcagatcg cagtgatcaa cggtgacgcg cgaaagatca actccgtggg
     8641 cttgcaacag agccagttgt tccagcccca tggtgctgaa cgaagtatgc gtggagatcg
     8701 ggcgtccggt ctggttatgc gccagcgcag cggcaataaa caccttctct tccagcggcg
     8761 taatcttccc ttcgctagag ccgatctccg cgataatccc ggctttcagg tcggtgccat
     8821 cgataccctg ttcaatttca tcgaccatct cctgcgccag ttcctgcacg ctgcgggtcg
     8881 ccacatgctc cgggaaaaaa gcgtcctggt aataaccggt gcaggccacc acgttgatcc
     8941 ccgtctcgcg cattacatcg agcataaatt gcgcattgcg ccccatgtaa cggttggtca
     9001 tctcaatcac attacgcacg ccccgggtca tcaggtcgtt catctcctgg caaatgaacg
     9061 catactgatc aaggcggcag tccacgttgt ttttaaagcc ggagagatca atatgcagat
     9121 gctcatggac cagggtgtaa cccgtcggat caaaactcat agggtgtctc ctgcaaaccg
     9181 gagggaggaa agaaaagagc gaccattttg tggcggtggc gcacggaaaa attcacacac
     9241 ggtagccccc acatctgaaa gcgtggtgcg cacaccgagc tgcgtagcta tcatcccttg
     9301 ctgataaacc agcactggca ccacttcgcg ggtatggtgg ctgtgaccaa tggtcggatc
     9361 gttaccgtga tccgccatca cgaccaggca atcatctggc tgcatcgcct caacaagccg
     9421 ggcaaggtta cggtcaacga cctgcaaacg ttcggcataa cgtgcgacgt cttctgcatg
     9481 accagcgagg tcggtttcct ggatattggt gcaaataaac gccgtcggat gcgtgttaaa
     9541 ttcgtcaagg gtgatatcca taatccgctg gctatccacc aggttttgcc agctcacgcc
     9601 ataaggattg ctgacgatat ctgccacttt acctaccagc acggttggca cgcccgcctc
     9661 atacagtttt tgcggcacct gcactttttc atcaacaccg tagcccatat gcacgacctg
     9721 gaaaccgttg tcataagcgc cagaacgcgg cgcattgatg ccaataaagc gcccttcttt
     9781 gctttctgcg gcatcgagaa tacgctgact gtcggttaac aagccgccaa atgtaatgac
     9841 ccgaccgacc tgtacctgct cacgcacgat acgaccaatt ttgattgcgt cgtcaaaaga
     9901 gatcacagag agattggcgg taatgttata gacctggcct aaatccgcct cgagattatc
     9961 gccaatcgca accgcctgat tgacccacag aaattgcagt tcatcgccac ggcgctccac
    10021 ctgccaaccg gcggatacta atgcctgctc aacacggtca atcacatcgc aaaaaggcat
    10081 ccgcagcggc ggtaacgggc gcgtgcctaa aatttcctga tgccccataa aggtatcgcc
    10141 accttcatgt tgcagctctg ccacgcccca ggttgcggaa tctgacggct gcatatcgcc
    10201 tggcgcataa cccaatgcgt tgattagccc cagcttctcc agcgttggta gctgcaaatg
    10261 cggcaactgg ctcaggatgt gaccacaggt attcgccccg gcatcttgcg gacgcaccag
    10321 cgtgacatct ttcattgccc ctacgccaaa gctatcaatc actaacacca caaatcgcgc
    10381 cattacgccc ccagagagtt tccaagactg tcataacgac caacgatttt cggttcgcca
    10441 cggtgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcggaaa
    10501 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    10561 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    10621 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    10681 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    10741 ccctgctggt ttgccggaat agtgcccgtc agcgcatgac cgggttcggc atgagtcaca
    10801 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggcgc gttcagttgc
    10861 tcaagtgcaa taccagattt cgccagttga tcccgtgcct gtatcagcgt gtgaagattc
    10921 ggtgtcggca aaacttttcc ggcagcctca tcccaaagca ggcaagggaa atgggtaagt
    10981 ccggctaaat gcagccccgg cagtttctta atttcagcaa ccacctcgtg cagcgaatgc
    11041 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    11101 agcacaaact gcacacgtcc ggccttcacc gctgccgcag aaacttcccg cgctttgtcg
    11161 agagtaaaca cggtgatgac gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    11221 gggatttgta ccagatgccc ctgatgcgcc accgacaaac cagcgcggcg catgactcgc
    11281 gcctctttgt aatccaccgc cacaatgccg ctgtaaccta atgccagcaa tttttccgcc
    11341 agccatgggt tacggccaaa ctgcttggtc atcagataca gttcaatgcc gtaaagccgc
    11401 gccgtctcaa tcagtcgctt accgttttcc agtacctgat ccacgtcgat cacccagctg
    11461 tccggggcga tcttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    11521 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    11581 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    11641 cgttagcagc catccataat ccataaaggt tggccagcaa atagccttct tcacacgggt
    11701 taacttccag tgcgaattcc ttcaacaata cctgatgcaa ttgcacaaca gcttgccagt
    11761 ggctggattg cgccagctct gccagcaatt cgttatccag cggctctatt tcttcaccac
    11821 ggcgactgcg catcagtgca ctcgccatat gtgtaatcgc catcgttcct tgctcactgc
    11881 gcactggcag atggcactct gtttccagta cgttgacgac ctgcatcatg cctttgcaga
    11941 tgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    12001 cctttcgttt aataactcgg ttcctgttcc ccactcacca cgcgttgttg atgggcgagc
    12061 agggcgtgtt tatctacaac ggcacgcagc agttgttgca ttgggtaatc atcggctcgc
    12121 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgtgag cggcgtcgcc
    12181 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    12241 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    12301 ctaccgccaa aaaaaacatc ggtcatgatt ttctgatccg ccgaacggtt atccagcccc
    12361 acgcgcttca cgtttgcgga ctcgccttta cggcaaatca actggtgctc gccaacgtag
    12421 gtgtgcggcc ccaactccag cgcgaggcat aacccttttt gcgtgagata actttccgcc
    12481 gccagtcggg aaaccaccgc catgtcatac acgccgttaa gtaaacactc cacgcgaata
    12541 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgagc tttcagcccg
    12601 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    12661 atatccacat gactcagcaa tgctttgtta tccatctcga ccagataact gccattgcgc
    12721 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    12781 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcaa cctattaccg
    12841 catttttcac ccaacaaata acgggccagc gtcgtgataa cgacgccttc ttttttgata
    12901 aacgttcgac gcataatata ttttcaataa aatgaatgtt tatatcttca ttaaaatgaa
    12961 gataagtgac aactgtgaga tgtggaaacg gcgaggcgct tcacattttt tcggattatt
    13021 gcaaatgagg ataaaaaaac cgggtttccc cggtttcaga gtgatgacga agtgatttat
    13081 tcgacaatgc gagcaaaggg taattcccac tggcgcttgc ctgctcccct caccctaacc
    13141 ctctccccaa aggggcgagg ggaccgtccg agcgttttga tattaaggct tcgccacaaa
    13201 accaatcgct tcgtacaccg ctttcagcgt acgggaagcg tgtacactgg ctttttctgc
    13261 gccgtctttc atcacctgtt gcaggaaggc ttcatcgttg cggaaacggt gatagcgttc
    13321 ctgcaattca gtcagcatac cggaaacggc atcagccact tcacctttca gatgaccata
    13381 catcttgcct tcgaactgtt tttccagttc tgggatgctc tggcccgtta ccgcagaaag
    13441 gatatccagc aggttggaaa cgcccgcttt gttctgcaca tcgtagcgaa ctaccggcgg
    13501 ctcgtcggag tcagtgaccg cgcgtttgat tttcttcact accgatttcg gatcttccag
    13561 caggccgata acgttattgc ggttatcgtc agacttggac atcttcttgg tcggctccag
    13621 cagcgacatt acgcgcgcgc cagatttcgg aataaacggc tccggcacct taaagatctc
    13681 gccatacagc gcgttgaaac gctgggcgat atcgcgactc agttccaggt gctgtttctg
    13741 gtcttcaccc accggtacca gattagtttg atacagcagg atgtccgcag ccatcagcac
    13801 cggatagtca aacagaccag cgttgatgtt ctcggcataa cgcgcagatt tatctttaaa
    13861 ctgcgtcatg cggctcagtt cgccgaagta ggtatagcag ttcagtgccc agcctaactg
    13921 cgcatgttct ggtacgtggg actgaacaaa aatggtgctt ttctcaggat cgataccaca
    13981 agccagatac aaggccagcg tatccaacgt cgctttacgc agcttctgtg catcctggcg
    14041 cacggtgatc gcgtgttggt caacgataca gtaaatgcaa tggtagtcat cctgcatgtt
    14101 tacccactga cgcagcgcac ccatgtagtt accaatggtc aattcacctg agggctgtgc
    14161 gccactaaaa acgatgggct tagtcatttt tcgattcctg attttcgcta tgcggaagcc
    14221 ctaatgcggg cagaaggtca tttatagact gataaattac atcaggctgg ctgagatcga
    14281 tagcctcgcc gtagttatat ccgtaggtta agccaactga tgggcaacct gccgcttttg
    14341 ccgcctgaat atcattgcgt gagtcgccga caaacagcat ctgttgtggg gcaattccca
    14401 tccgctcagc caccagtaac agcgggtccg gatgcggttt tttgttttgc acatcatcgc
    14461 cgccgataac cacgctaaag tatttggcaa tatctaaggc ttcgagcagc ggcgcgacga
    14521 acggcgtcgg tttgttggtg accaggccta gcggcaggcc tttagcctgc aacgcgccca
    14581 acgtatcggc aacgtgcggg aacaaaaacg tcccctcttc ggcaacctcg ccatagtagc
    14641 gatcgaacag tttacgcaga atacgtacct gttcttctgc cggaatgtcg tcatcaacgg
    14701 gcggtttacc cattgtttta cgctgagtcg cacgttcctg acgcgcccag gtcaatgcgc
    14761 gctccatcag aacatctgcg ccgttaccaa tccaggtaat aacgcgttct tcacctgcga
    14821 cgggcaactc cagcgcatac agcgccatat ctaccgcagc agcaagaccg ggagcactgt
    14881 cgaccagcgt accatcaaga tcaaaagcga cgccgcgaat atcttcaaac ttattcatga
    14941 cttacctttg ccagttcact gcgcatttca tcaatgactt ttttgtagtc tggctggtcg
    15001 aagattgccg aaccggcgac gaacatatcc gcgcccgccg cagcgatttc gccaatgttg
    15061 ttcaccttca cgccaccgtc tacttccaga cgaatgtcaa agccagactc gtcgatacga
    15121 cgacgtactt cgcgcagttt atccagtgtt tgaggaatga acgactgacc gccgaaacca
    15181 ggattgacgg acatcagcag gatcacatcc agcttatcca tcacgtaatc cagatagctc
    15241 agcggtgtcg ccgggttaaa taccagtccc gctttacagc cattttcttt aatcagttgc
    15301 agcgtgcggt caacatgctc ggaggcttct ggatgaaagg taatgatgct ggcaccagcg
    15361 gcagcgaaat caggcacaat gcgatcgaca ggtttcacca tcaggtgtac gtcgataggg
    15421 gcggtaatgc cgtagttacg caaggatttc agcaccattg gcccaatcgt cagattggga
    15481 acatagtggt tatccatgac gtcaaaatgc acgacatcag cgccagctgc cagggctttt
    15541 gcggtatctt cacccaggcg ggcaaaatca gccgacagaa ttgagggggc aatcaaatac
    15601 tgtttcatcc gcttctcctt gagaattatt ttttcgcggg tgaaacgact cctggtttgt
    15661 acaaagccag cagttcgtcc acctttttac gtgtgccgcc gttgctgctt atactgcgtc
    15721 gaactttgac gacatgcaat tttgcgcgct gataccactc acgcgttaac atcgtatcgt
    15781 gattggagat cagcactgga atatggcgct caaccagacc ttcggcgatc tccgccagat
    15841 gcgcttgttg ttcaagcgta aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag
    15901 acagcggtgc ataaggcgga tcgcaataga cgacggatgc atcatctgcg cgcgccatgc
    15961 tatcggcgta agactcacaa tagaaaaagg cattctgcgc tttttcagcg aagtgataca
    16021 actctgcttc cgggaaatag ggttttttgt agcggccaaa cggcacgtta aactcaccgc
    16081 gcagattgta acgacacagg ccgttgtaac cgtagcggtt caaatataaa aacagtaccg
    16141 cccgacggaa cggatcctgg cttttgttga actcttcgcg gaactgatag taaacctcgg
    16201 cgcaatttgt ttcgggaaca aacagctcgc gtgcggcctg tacgtactca tcagtacgca
    16261 tcttcacaat gttatagaga ctgatcaggt cgctattgat atcggcaagg atataacgag
    16321 aaaagtcggt gttgagaaac accgacccgg cacctacaaa aggctcaacc agacattcgc
    16381 ccttgggcaa atgccgttta atatcatcaa gcaggggata cttgccccct gcccacttca
    16441 aaaaagcgcg atttttcttc atgctgacta actaattaca ccttctccgg ctgtggagaa
    16501 agctccgaca gcatccagcg cttcaggcaa ctcccgcaac gataaaccat tgcgggagat
    16561 aaccttgatt acttcagatc ggcctgtacc tgacgcagcg gtttcgccca cgggtttttg
    16621 gcctgaacat ctgctggcaa tgtagatacc gcttttttcg cctcttcttt cgaagcgtac
    16681 acgccagaaa ccaggacata ccacggctga ccattacgcg tcgtttcata gacaacgtag
    16741 tttttcagat tctctttctt cgcccaaccg ttcaggttgt cgtagttaga ggaactgctc
    16801 agctgcagag tgtaatggct ggacggtgcc gatttcaacg aaccaacatt acctgcggtc
    16861 ttccctccag cggctggtgt tgccgtggtt tgcgccgggg atgccgtctg tactggagcc
    16921 gtagtcgccg tctcttttgg tgctggcgta gaagtcgcag ccggtgcctt cgtgctcgcc
    16981 acaggggcag ctggttcagt acgcttcggc gtctgtgcta ccggcttcgg ctccgttttc
    17041 acggttgctt gcggtttttt cggttcaatc accgcctgct gacgagctgg gcgcgtagtg
    17101 gacggacgtt cagcggtttg cgttttcgcc gtgtcacgcg atgcattgcc attgcgaaca
    17161 ggcgcgacag tcgcgggttc ggtcggcaat gtggaattga ccgccacatt gttcaactgt
    17221 tgctgatttt gtggctgggt cagcgcattg ttcaggtcac cctgcacttc aacacgttgt
    17281 tgaccatccg ttgccgccgg ggtttgccct tgagtcggcg tagaagagat cggtggcaga
    17341 gaaacatcct gctgagtatt ttccgcagac gtggttcccg gcgctggctg cacgccattc
    17401 gcctgatcgg tcgcattgcc tgcaagatca atactcttct cgccagacgc ggtttgatcg
    17461 ctggaagagg tcgagggggc ttttagcgca gaaccgatac cgatgatcaa cagcagtaga
    17521 accagaatgc cgacgcccat catcatatac tgacgagaag cgggtttact ggctgctttt
    17581 ttgcgcttac gcggacgacg ctctacgcgc tcttcatcca cggtttcatc ttcggattcg
    17641 tcaatttctt cttcgatttc cggttcctca ttgcgctctt tttgcgcacg agtcggacgg
    17701 cgatcgtcag tgtcatcaag ttcaatatca tcaaaattga tctgcggttc gccacgttca
    17761 gtacgctcag aacgttcaga agattgacga gaacgaccag tacgacgatc gctgggatcg
    17821 ggtttcagct cgtcttctgg tttgaattca tccatttaac accccactaa aaggttaatg
    17881 cttaccacgt tgcaattaac ctgaagctaa tagaccgctt gataagcggc ctgacctttc
    17941 ttgttgttac gctgattgac aatcggcaat ggcgttaaga acaagctcgt gcgaaacgcc
    18001 gctgcgaact tcactcttac caattgccaa cggaagaatt aagcgtatct ctcccgcaag
    18061 gactttcttg tcacgcagca tatgcggtaa atacgcctgc gcggacattt cgcgcggccc
    18121 attgaccggt aacccagccc gcttgagcag ggttataata cgctgcgttt cggcagaact
    18181 aaactgcccg agacgttccg acgtccgcgc cgccatcacc atacccgcag cgaccgcttc
    18241 accatgtaac caattgccat accccatttc agcttcaatg gcatgaccaa aggtgtgtcc
    18301 cagattcagt aaagcacgta acccggtttc gcgctcgtcg gcggcgacaa cttctgcctt
    18361 cagttcacaa caacggcgaa tacagtacgc cattgccgga ccgtccagac gcaacaacgc
    18421 atccagattc tcttccagcc agttaaaaaa cgcaccgtca agaataatgc cgtatttgat
    18481 gatttctgcc agccccgacg ctaactcacg cgggggaagc gttttcagac agtcgagatc
    18541 caccaccact gaagcaggct ggtagaacgc gccaatcatg tttttaccga ggggatggtt
    18601 gaccgcagtt ttgccgccaa cggaggaatc gacctgcgac agtaacgtcg tcgggacttg
    18661 aatgaaacga acaccgcgct gataactcgc cgccgcgaag ccggtcagat cgcccactac
    18721 gccgccgcca agcgccacca gcgtagtatc gcgaccatgc ggcttttgta acaacgccgt
    18781 aaagacggta tcgagtacag ccaggctttt atactgctcg ccgtcaggga ggataacgct
    18841 atcgacgtta acacccgcct gttcaagtac gccgcggacc ttatcgagat acagaggagc
    18901 cagggtttcg ttggtgacca acatgacctg ctcgcccgat ttcagcggta agaatgaagc
    18961 tggttcatta aacaaaccag atgcgatggt aattgggtaa ctacgttccc cgagagtaac
    19021 gacaatcctc tccataacgc gacatccacc ttaattactg tacccgcaga cgagtgtata
    19081 taaagccaga attagttgct ttccagcatg tgaataatct ggtttgcaac cactttagcg
    19141 ctttgatcat cagtacgaat ggtcacgtcg gcaatctctt catacagcgg attgcgttca
    19201 ttggccaacg cttccagaac ttcacgcggc ggtgtttcaa cgtgcagcaa cgggcgtttt
    19261 ttatcacgct gcgtgcgtgc aagttgcttt tcgatggtcg tttcaagata aacgacaacg
    19321 ccacgagcgg aaagacggtt acgcgtttca cgggatttca cagagccgcc gccagtagcc
    19381 agcacaatac cctgtttctc ggtcaactca ttgatgacct tttcttcgcg atcgcggaag
    19441 ccttcttcgc cttctaaatc gaaaacccag cccacatcag ctccggttcg tttctcaatc
    19501 tcttgatcgg aatcgtaaaa ttccatattg agttgttgag ctaactggcg cccaatagtg
    19561 ctttttccgg cacccatagg cccaaccaga aagatattgc gtttctctgc cattttttcg
    19621 gtactactaa gactattcgt taatgataaa cccgcttcgc tcagagagcg ccgcaggaca
    19681 tgaactgaaa cctcataaga tattgcgaga gtcagactga aaattatctc aatactccag
    19741 cgggtttggc aactgaataa atcacccatc cctctgcata tctggtcgct gcaagcgcgc
    19801 tgccttgcta ccaccgctct ggcgataaat caccgggtaa gattagcgta aaaaagacag
    19861 caaaatgccg cctgaatgat aaatcatcat catggaacgc aggcagcgaa gagcaccaac
    19921 tctcaaatcg gtactccttg tatgctaaat acctgcgcgc gtcaaataga tgaaacacgt
    19981 tcagcataaa aacattaccg cttttacggc tgtttactca ctggaaacca gtcgtggcgt
    20041 gataaacacc actaactcgc gtcgttcatc ttcttttccg tcatgacgaa ataattgccc
    20101 gaaccaggga atgtcgccaa gcaacggtac gctatcctga cccgatttat ttttacgggt
    20161 aaaaatgccg cccagcgcca acgtttctcc gcttttgacc tcgacctgcg tttcgatctc
    20221 ctgcttatca atcgccagca cttcgccatc ggcctgctgt agcacctgcc ccggaacgtt
    20281 ctggctgatg tgtaatttca gccggatgcg acctttttgt aacaccgtgg gcgtgacctc
    20341 catccccagg acggcctctt taaattccac cgacgtcgcg ccactttccc cgctggaaac
    20401 ctgatatgga atttcgctcc cctgtttaat gctggcaggc tgaagatgtg aggccagcag
    20461 acgcggactg gcgataatat ccagctgctg tttttgttcg agcgcagaaa gctcaagatc
    20521 cagcaagcgt ccgttgatgc gcccaatgtt aaaaccgaca tgcgttgtcg ccgtcgctac
    20581 ggagaggtcg ctaccaagcg tggtgacttg cccaacgcca ccagcgtgtt gcgcatcggc
    20641 cagcgtccat ttcacgccta actcacgcaa acttttttca ttaatggtga caatatgcgc
    20701 cgacagctca acctgcccga ccggcagatc catttgcgct acccactgtt caagcgcgct
    20761 taacgccgtt ttgttatctc gtagcaaaag gcgattggtg cgtttatcga cggtcatact
    20821 ccctttggca ctcagtagct tctcccccgc tttcgccagt tctcccgcgt cggcgtattg
    20881 cagggttata ctgcgatttt ccagcggcag atttgcctgc gcccgcgcct gctccgcctc
    20941 ctggcgggcg atattgttat tctgccaggc aatggaatgc actgagagaa tgttgtcttc
    21001 ctgccgcgtt atcagtccgg cgcttttcac tacagtttgt agtgcctgct tccagggaac
    21061 atctgttaga tgtaacgaca ccgtaccgct gacgtctggc gacacgacca ggttcaactt
    21121 ctcctgttca gccagcgcct gcaacacctg agctaccgga acgtcatcca ccatcagcgt
    21181 cactttttgc ggctttgccg cctgtacgcc gggtatcagc atcaacagta gtgcggctat
    21241 ccattgcttc atttgtatct ccttgccgtt gccacaacca ttgtggcggt tcgcagtttg
    21301 tcccggtacc cagcgttagt acgtctggcg tcagctgtaa aattgtccag ccgttttcca
    21361 gcacatcgtt ttgctgcacc cgtcgccatt tcttttgccc gtcttttatt acaccgatga
    21421 tgcgctcgcc tcgccctacc atcccctgat agcgccactg gctaagttcg ctaatccggc
    21481 atagatcttc cggcggttta aaagggtcac gcataccggt taaaaggcac aatgcaatac
    21541 ctgccaacaa ccagcgttta accctcatgc ggcgtctcca gttgtagcgt gaacaaaaga
    21601 tcatcacctt ccacgcttaa cgaaaaacgg ctcacgctga cgttgcgctc tgccagccgt
    21661 gtaaatgccg acggcactgc ttcccacagc gttttcaacg ccaactcgcc tccctgcgcg
    21721 gatggatgcc aggaaaccag ttgcgcgccg gataactgaa aatccagtgg cgaaaagggc
    21781 agcgtttttt cctcgctgaa gggagtggtg tctaccaggc gatacagtgc ggcccactgc
    21841 tggtgatgac ttgcccgtag tcgaattaat gcgtcgcgct cttcatggtg tgtcgatgac
    21901 agaaaaatga gcgtaactaa catcagcaac cagaatgccc agcaaaactg gcggaggcgg
    21961 ggtgatgtgg cgaaccacca gtcaaagaac atgttcatcg ctaaccttcc ttgttaactg
    22021 atactcaaat tgccagcgtc cctgcgcatc ctgctgcgtg gccccccgct gattgagatg
    22081 aaaagaagca tcctggcgaa gtgacgtttc tagtgcgttt aacgcggtaa tgcttgttgt
    22141 aagccccttg atctccagcg ttccctgctg ccagcttatc gttgtcagcc aggcgtgttc
    22201 aggtaaaaga gccgccagtg cttccagcgc agattgccag tcgcgggtaa attgtcgctg
    22261 gcgctgcctt tgcaaacgct gctcacgaat ttgttggcgc tccagcaaac gtggtttcgt
    22321 tatctgtaag ctgtgggcga gttgttgttc cccctgaagc aaaacggcgt ctacgcgagc
    22381 ttcggcgctg cttgtcagac gcagtattag cgttatcccg acggccagca gcagaggcgc
    22441 aacgaacatc agcaaccaga aacgcagaaa agcggtccgg cgttgctgtc gccagggcaa
    22501 aaaattaatt ggcaggttca tcagtacgcc ttcccaagcg ccagccccag cgcgatggca
    22561 aagcctccac ctggcggcgg tagcggcggc tgacgaacag aaacggcctc ccagggatca
    22621 aatccgcctt cgccacatat cgcgacgctg tcgggatcaa cggataacgc tgccgccagc
    22681 tccttcgcgc tagtcatccc taccgccagt ttgcgccccc agcgatagcg tgtcgcccac
    22741 agccactgtt cgttatcacg ccaggccaga cattgctgat gagaaggtaa aaaaggcagg
    22801 aattgctgta atgcactggc atccggggtg atcgcactaa catgaacacg caacctttct
    22861 gccagcgtaa gcagcgttgc cagctctttg ctttgcgcgg cagtcacgtt ataagcgggg
    22921 ctgagtgagt cttcgctata atcgaagcgc agggagtccg gatccatatc cagctcgcgg
    22981 gccatcgtcc ctgacagcca ggccgtttgc tcccgctcac caagggacat cgacgggcgc
    23041 ggaaatgacc gctgtaatgt gcgactggcg ggaaacgcca acataatgtg atgacgctgc
    23101 ggcagttcgc gactccaagg taacaacgtt ttagccagct gctgcgcatc aacaatccgc
    23161 ccatctttga taatgtcgtt ctccagcggc aaccgccacc agcgttgcaa aaagcattct
    23221 tttgcgcccc gtacgatcgc aaccgctacc gcttcttgct gttgtaaatg caaaccaatt
    23281 tgccagatct taaatgccat tgtgatgatc tccttatcac ccgtcactct gacgggtata
    23341 tcaatgcgtc tggcttgcct ttatactacc gcgcgtttgt ttataaactg cccaaatgaa
    23401 actaaatggg aaatttccag tgaagttcgt aaagtatttt ttgatccttg cagtctgttg
    23461 cattctgctg ggagcaggct cgatttatgg cctataccgc tacatcgagc cacaactgcc
    23521 ggatgtggcg acattaaaag atgttcgcct gcaaattccg atgcagattt acagcgccga
    23581 tggcgagctg attgctcaat acggtgagaa acgtcgtatt ccggttacgt tggatcaaat
    23641 cccaccggag atggtgaaag cctttatcgc gacggaagac agccgcttct acgagcatca
    23701 cggcgttgac ccggtgggga tcttccgtgc ggcaagtgtg gcgctgttct ccggccacgc
    23761 gtcacaaggg gcgagtacca ttacccagca gctggcgaga aatttcttcc tcagtccaga
    23821 acgcacgctg atgcgtaaga ttaaggaagt cttcctcgcg attcgcattg aacagttgct
    23881 gacgaaagac gagatcctcg agctttatct gaacaagatt tacctcggtt accgcgccta
    23941 tggtgtcggt gctgcggcac aagtctattt cggtaaaacg gtcgaccaac tgacgctgaa
    24001 cgaaatggct gtgatcgccg ggctgccgaa agcgccttcc acctttaatc cgctctactc
    24061 aatggatcgt gccgtcgcgc ggcgtaacgt cgtgctgtcg cggatgctgg atgaagggta
    24121 tatcacccaa caacagttcg atcagacacg cactgaggcg attaacgcta actatcacgc
    24181 gccggagatt gctttttctg cgccgtacct gagcgaaatg gtgcgccagg agatgtataa
    24241 ccgttatggc gaaagtgcct atgaagacgg ttatcgcatt tacaccacca tcacccgcaa
    24301 agtgcagcag gccgcacagc aggcggtacg taataacgtg ctggactacg acatgcgcca
    24361 cggctatcgc ggcccggcaa atgtgctgtg gaaagtgggc gagtcggcgt gggataacaa
    24421 caagattacc gatacgctga aggcgctgcc gacctatggt ccgctgctgc ctgccgcagt
    24481 caccagcgcc aatcctcagg aagcgacggc aatgctggcg gacgggtcga ctgtcgcatt
    24541 gagtatggac ggcgttcgct gggcgcgtcc ttaccgttcg gatactcagc agggaccgac
    24601 gccgcgtaaa gtgaccgatg ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg
    24661 cgatgcatgg tggctggcac aagtgccgga agtgaactcg gcgctggtgt cgatcaatcc
    24721 gcaaaacggt gccgttatgg cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa
    24781 ccgcgccacc caggcactgc gtcaggtggg ttccaacatc aaaccgttcc tctacaccgc
    24841 ggcgatggat aaaggtctga cgctggcaag tatgttgaac gatgtgccaa tttctcgctg
    24901 ggatgcaggt gccggttctg actggcagcc gaagaactca ccaccgcagt atgctggtcc
    24961 aattcgctta cgtcaggggc tgggtcagtc gaaaaacgtg gtgatggtac gcgcaatgcg
    25021 ggcgatgggc gtcgactacg ctgcagaata tctgcaacgc ttcggcttcc cggcgcaaaa
    25081 cattgtccac accgaatcgc tggcgctggg ttcagcgtcc ttcaccccaa tgcaggtggc
    25141 gcgcggctac gcggtcatgg cgaacggtgg cttcctggtg gacccgtggt ttatcagcaa
    25201 aattgaaaac gatcagggcg gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga
    25261 atgcgatatt ccggtgattt acggtgatac gcagaaatcg aacgtgctgg aaaataacga
    25321 tgttgaagat gtcgctatct cccgcgagca gcagaatgtt tctgtaccaa tgccgcagct
    25381 ggagcaggca aatcaggcgt tagtggcgaa gactggcgcg caggagtacg caccgcacgt
    25441 catcaacact ccgctggcat tcctgattaa gagtgctttg aacaccaata tctttggtga
    25501 accaggctgg cagggtaccg gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg
    25561 cgggaaaacc ggtaccacta acagttcgaa agatgcgtgg ttctcgggtt acggtccggg
    25621 cgttgtgacc tcggtctgga ttggctttga tgatcaccgt cgtaatctcg gtcatacaac
    25681 ggcttccgga gcgattaaag atcagatctc aggttacgaa ggcggtgcca agagtgccca
    25741 gcctgcatgg gacgcttata tgaaagccgt tcttgaaggt gtgccggagc agccgctgac
    25801 gccgcctccg ggtattgtga cggtgaatat cgatcgcagc accgggcagt tagctaatgg
    25861 tggcaacagc cgcgaagagt atttcatcga aggtacgcag ccgacacaac aggcggtgca
    25921 cgaggtggga acgaccatta tcgataatgg tgaggcacag gaattgttct gattaaaaag
    25981 gcgcttcggc gccttttcag ttggctgaca aagtgcattt gtttatgccg gatgcgggtg
    26041 aacgcgttat ccggctaaca aaatcgtgaa aaatcaatat attgcaggaa acccataagc
    26101 ctgataagcg ttgtgcatca ggcaaacttc acgcatttac actcgcccct gccctttcaa
    26161 ccattcgcgc acgaggaaca gcgcgctgac attacgcgct tcgttgaagt cagggtcttc
    26221 cagcaaatcc atcatatgcg ccagcggcca gcgcacctgt ggtagcggct ctggctcatc
    26281 gccttccagt gattccgggt agagatcttg cgctaccacg atattcattt tgctggaaaa
    26341 ataagacggt gccatgctga gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac
    26401 ctcttctttt agctcgcggt tagcggcttc gtagacgctt tcacccggat caattaaacc
    26461 tttcgaaaaa cctaattcgt aggattcagt tcccactgcg tattcgcgga tcaggatcag
    26521 gtgatcgtcc acaatcggca caatcatcac tgcttcccgg ttggttggac gcattcgttc
    26581 ataaacacgc cgcacgccat tgctgaactc cagatccacg ctctcgacgg taaacagtcg
    26641 ggaacgggct acagtttcaa cattcagaat ggtgggtttt tgtaatgatt tgctcatcgt
    26701 gggatctatg ctgtgaaatc agcggttatt gtgcgatatc ggacacgctt tcggcaatgt
    26761 gaattgcatg ttatttacat ttatgtaact taataaataa tcgtcctcaa atcaaattaa
    26821 aagtcaatag gttgaaataa ctccaggaat ttgctgatat tccgccttca gagggtttgc
    26881 tatgatcagc ggttactgtg atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg
    26941 atagccaacc gcagaatcat gtattgtgtc tggtgcgact gaccacgcct gacagactaa
    27001 gtaagatggg gaaagcatga gcaccattgt gattttttta gctgctttgc tggcctgctc
    27061 actacttgcg ggatggctga taaaagtgcg atccagacgg cgtcagctgc cctggaccaa
    27121 cgccttcgcg gatgcgcaaa cgcgtaaact cacacctgaa gaacgtagcg ccgttgaaaa
    27181 ttatcttgag agcctgacgc aggtattaca ggtgcctggc ccaacgggag ctagcgcggc
    27241 accgatctct ctggcgctga atgccgaaag caacaacgtc atgatgctga cacacgctat
    27301 cacgcgttac ggcatctcta ccgacgatcc gaataaatgg cgttactacc tcgattcggt
    27361 agaagtccac ctgccccctt tctgggaaca atacatcaac gatgagaata ccgttgaact
    27421 gatttatacc gattcgctgc cgctggttat ttcactcaac ggtcatacgc tgcaggagta
    27481 catgcaggaa acgcgcggct atgccttgca acctgttccg tccacgcagg cgtcgattcg
    27541 cggggaagag agtgagcaaa tcgagctact caatattcgc aaagaaacgc atgaagaata
    27601 tgcgctgagt cgtccgcgcg ggctgcgtga agcgttgctg atcgtcgcct ccttcctgat
    27661 gttctttttc tgcctgatta ccccggatgt atttgttccg tggctggcag gcggcgcatt
    27721 actgctgctg ggcgcaggtc tgtgggggct attcgcgccc ccggcaaaat cctccctgcg
    27781 agaaattcat tgtctgcgcg gtacaccccg tcgttgggga ttgtttggcg aaaacgatca
    27841 ggaacagatc aacaatattt cactcggtat tatcgacctg gtctatcccg cacactggca
    27901 gccatacatt gctcaggatc tcggtcaaca aaccaatatt gatatctatc tcgaccgcca
    27961 cgtagtgcgt caggggcgat atctttcgct gcatgatgaa gtaaaaaact ttccgttaca
    28021 gcactggctg cgcagtacga ttatcgctgc gggctcgctg ctggtgctgt ttatgctgtt
    28081 attctggatc ccgctggata tgccgctgaa attcactctc tcatggatga aaggcgcgca
    28141 gaccattgaa gccaccagcg taaaacaact ggctgacgct ggcgtgcggg taggcgatac
    28201 attgcgtatt agcggtacgg gaatgtgtaa tattcgcact tccggaacct ggagcgcgaa
    28261 aaccaattca ccttttttac cgtttgactg ctcgcagatc atctggaatg acgcccgctc
    28321 attaccatta ccagaatctg aactggtcaa caaagcgacg gcattgactg aagcagttaa
    28381 tcgccagctg caccctaaac cggaagatga atctcgcgtc agtgcctcat tacgttcagc
    28441 gattcaaaaa tccggcatgg tattgcttga tgattttggc gacattgtac tgaagacagc
    28501 ggatttatgt tctgccaaag atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg
    28561 caacagtaaa gactgggacg cgctggtaaa acgcgccaac gccgggaagc tcgatggcgt
    28621 gaatgtgtta ttacgcccgg tgagtgcgga atcgctggat aacctggtgg caacctccac
    28681 cgcgccgttt atcacgcatg aaacggcgcg agcggcacaa tcactaaaca gtccggcccc
    28741 cggcggattc ctgattgtca gcgacgaagg cagcgatttt gttgatcagc cctggccttc
    28801 ggcatcactt tacgactacc cgccgcaaga acagtggaac gctttccaga aactggcaca
    28861 aatgctgatg catacgccgt ttaacgccga aggtatcgtc acaaaaatct tcactgacgc
    28921 caatggtacg cagcatattg gccttcatcc gatcccggat cgttccggcc tgtggcgcta
    28981 tctcagcacc acattgctgc tactgacgat gctgggtagc gccatttaca atggcgtaca
    29041 ggcctggcgt cgttaccagc gtcatcgcac tcgcatgatg gagattcagg cctattatga
    29101 aagctgcctg aacccgcaac tgatcacccc ttcagaaagc cttatcgaat aacacgtttg
    29161 cgcggcaggt tatgctaccc tgtcgcgcaa attgcttcac tctggagatt tccctcatgc
    29221 atatcaacat tgcctggcag gacgtagata ccgttctgct ggatatggac ggcacattgc
    29281 tcgacctcgc cttcgataac tatttctggc aaaagctggt gcctgaaaca tggggcgcga
    29341 aaaacggggt tacgccacag gaagcgatgg aatatatgcg ccagcaatat cacgacgtac
    29401 agcatacgct aaactggtac tgtcttgatt actggagtga gcaactgggt ctggatatct
    29461 gtgcgatgac caccgagatg ggaccgcgtg ccgtactgcg tgaagatacc attccgtttc
    29521 ttgaggcact gaaagccagc ggtaagcagc gaattttgct caccaatgcg catccgcaca
    29581 acctggcggt aaaacttgag cataccggtc tggacgcaca ccttgattta ttactttcca
    29641 cccacacatt tggttatccg aaagaggatc agcggttatg gcatgcggtg gccgaagcta
    29701 cgggtctgaa agctgaaaga acgctgttta ttgatgacag cgaagcgatt ctcgatgctg
    29761 ccgcgcaatt tggtattcgt tactgcctcg gcgtgactaa tcctgattcc gggattgccg
    29821 agaaacagta tcaacgccat ccgtcactga atgactaccg ccgcctgatc ccctcgctaa
    29881 tgtgaaggag acgccatgaa agaaaaacct gctgttgagg ttcgactgga taaatggcta
    29941 tgggctgccc gtttttataa aacccgcgcg ctggcccgtg aaatgattga aggcggtaag
    30001 gtgcattaca acgggcagcg cagcaagccg agcaaaatcg tcgagctgaa tgccacgctc
    30061 actctgcgcc agggaaatga cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt
    30121 cgccccgcca gcgaggcagc cttgctgtat gaagagactg cggaaagtgt agagaaacgc
    30181 gaaaaaatgg cgatggcacg taaacttaat gccttaacca tgccgcaccc ggaccgacgc
    30241 ccggacaaaa aagagcgccg cgacctgtta cgatttaaac acggcgacag tgaataactg
    30301 tcacctgcaa gagagatgat tatgccgcaa catgaccaat tacatcgcta tctgtttgaa
    30361 aattttgccg ttcgcggcga actggtaacc gtttcggaaa ccctgcaaca gatccttgag
    30421 aaccacgatt atccgcagcc cgttaaaaac gtgctggcag aactgctggt tgcgaccagc
    30481 ctgttaaccg ctacgctgaa gtttgatggt gatatcaccg tacagctgca aggcgacggt
    30541 ccgatgaatc tggcggttat taacggtaac aataaccagc aaatgcgcgg tgtggcgcgc
    30601 gtgcagggcg aaattccaga aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg
    30661 gtgatcacca ttaccccgag cgaaggcgaa cgctatcagg gcgtggttgg tctggaaggt
    30721 gataccctgg cggcctgcct ggaagattac tttatgcgtt ctgaacagct gccgacgcgc
    30781 ctgtttattc gcaccggcga cgtagacggc aaaccggctg caggcggtat gttgttgcag
    30841 gtaatgcctg cgcaaaatgc ccagcaggac gactttgacc acctggcgac gctaaccgaa
    30901 accatcaaaa ccgaagaact gctgacctta ccggcaaacg aagtgttgtg gcgtttgtat
    30961 cacgaagaag aggtgacggt ttacgatccg caggatgtgg agttcaaatg cacctgctcg
    31021 cgtgaacgtt gcgccgatgc gctgaaaacg ctgcctgatg aagaagttga tagcatcctg
    31081 gcggaagatg gcgaaattga catgcattgt gattactgcg gtaaccacta tctgttcaat
    31141 gcgatggata ttgctgaaat ccgcaacaac gcgtctccgg cagatccgca agttcattaa
    31201 tgagtatgtc cggcagagag ggctctgccg gattttacga cgtaatggcc tgatgtgctg
    31261 cgcttaccag gcctgtgaaa taaatcgcaa attattgttg gtcagataca gcgtcgtatc
    31321 cgttaaaaga gttgtcactt acttaagttt atttccagcg tttcaccatc taccgtcaga
    31381 tcgcgccacg ctttcggcct cgtccccagt tttccatcct tcgcagctgt gagtaatttt
    31441 tctttcgtta tttcgttcgg aagtaaactc atatcaagcg cgtcccattc ttttctgtct
    31501 gagtcaaagc catagacaat gactctgtca tcattaaaag caaacaggat ccgctccgcc
    31561 tgaccatcac tattcagatc ttgctcgacc aggacgcagg cgtctttttc aatacaggtc
    31621 atcacgttat agcgatcctg gattaaggcc gaccagaatg tcgcatccgg tttaacagaa
    31681 ccaggggcaa ttaacacatt ttcggctaat actttttccg atacctgttc ctgaagatgt
    31741 tgctctccat ctaacgccat cagcagatcc cgcgcgcgtt tcgggtcttt cataaacccg
    31801 gcatcgcttt tcagcgactc aagcgccgca cgtccatagc gaccgctctg ttcgagcatg
    31861 tagatagtta cctggtctgg cgtgtttttg ccgctctgat aacgcgccat atgactgttc
    31921 acgctaatac gcatactgtc cagcaccggc gaattaagaa gcaccagtat caccaacacc
    31981 aataacgaaa ccgcaaggtt cactttgccc tgaagaacaa ggggattttg cccattacgc
    32041 cagacgatac tgacaaaata ccccagcgac cacaccagta acaccagcac cgccagcgcg
    32101 ccctgcaagc ggtcgacagt ccagccgtac tgagcgaccc gcagccataa cgcccaggcg
    32161 gcgacgaaca catacagcgg agcaaccagc aaagcggttt taatcaggca acgcaacggc
    32221 cccgtccagg gaagtgacgc tttttgcgga tcgcggacaa tagccattaa gatcaattgc
    32281 aaaaaggcca gcgttaacag caacccggcg gcggagatgt ggcgagaaat cgcgctcagg
    32341 cccgtaaacg gcagagtgat gataaacatc agggttagca atgatactaa cggcagcaac
    32401 cccgtggcga ttaacgtgaa caacttttga atagagtcga ttaaacgtga ctgtgtgcgc
    32461 gcgagaatca ccgccagtgc ggtaaccaga cctaatgtaa gatacataaa ccagtcggtt
    32521 gcaaaaaaga gcgtattgaa aaacttgatg ccaacaagtt taaacaactc accccacagt
    32581 aaaagcacca gccacgtcag gccattggcg agaaaaatca ccagtaatat aagtacgtta
    32641 tgccatactg actggtaaaa gtagctatag cggctactgc cattgcggat acgaaggctt
    32701 tgttgtatcc acggtagcaa caacattgcc atcaacagca gatagcagcc aaaatcccaa
    32761 atcgcctttt cagctctcca ggggttcatg ccatcagttt gccacttcag ccaaccgctc
    32821 atccctgtcg tggcaataaa caccagcgcc agccatcccc aaaggcgctt ctgtttaaaa
    32881 gagatcacgg agaagagtaa aacggatgaa aacgcaacgg tcgcaggcac gccataaaca
    32941 atccaactgt gattttttcc ggcaagccag gcgatcaaca ggtagcaaac cagcccctga
    33001 agtaatccgg tagcaatcat cccccagcgt gttgccggtg aaagttcaac gttatccatg
    33061 ttggttatcc agaatcaaaa ggtgggttaa ttatcgcatc cgggcagtag tattttgctt
    33121 ttttcagaaa ataatcaaaa aaagttagcg tggtgaatcg atactttacc gattgaattt
    33181 gcatcaattt cattaaggaa tgcgattcca ctcacaatat tcccgccata taaaccaaga
    33241 tttaaccttt tgagaacatt ttccacacct aaaatgctat ttctgcgata atagcaaccg
    33301 tttcgtgaca ggaatcacgg agttttttgt caaatatgaa tttctccaga tacgtaaatc
    33361 tatgagcctt gtcgcggtta acacccccaa aaagacttta ctattcaggc aatacatatt
    33421 ggctaaggag cagtgaaatg cgcgttaaca atggtttgac cccgcaagaa ctcgaggctt
    33481 atggtatcag tgacgtacat gatatcgttt acaacccaag ctacgacctg ctgtatcagg
    33541 aagagctcga tccgagcctg acaggttatg agcgcggggt gttaactaat ctgggtgccg
    33601 ttgccgtcga taccgggatc ttcaccggtc gttcaccaaa agataagtat atcgtccgtg
    33661 acgataccac tcgcgatact ttctggtggg cagacaaagg caaaggtaag aacgacaaca
    33721 aacctctctc tccggaaacc tggcagcatc tgaaaggcct agtgaccagg cagctttccg
    33781 gcaaacgtct gttcgttgtc gacgctttct gtggtgcgaa cccggatact cgtctttccg
    33841 tccgtttcat caccgaagtg gcctggcagg cgcattttgt caaaaacatg tttattcgcc
    33901 cgagcgatga agagctggca ggtttcaaac cagactttat cgttatgaac ggcgcgaagt
    33961 gcactaaccc gcagtggaaa gaacagggtc tgaactccga aaacttcgtg gcgtttaacc
    34021 tgaccgagcg catgcagctg attggcggca cctggtacgg cggcgaaatg aagaaaggga
    34081 tgttctcgat gatgaactac ctgctgccgc tgaaaggtat cgcttctatg cactgctccg
    34141 ccaacgttgg tgagaaaggc gatgttgcgg tgttcttcgg cctttccggc accggtaaaa
    34201 ccaccctttc caccgacccg aaacgtcgcc tgattggtga tgacgagcac ggctgggacg
    34261 atgacggcgt gtttaacttc gaaggcggct gctacgcaaa aactatcaag ctgtcgaaag
    34321 aagcggaacc tgaaatctac aacgctatcc gtcgcgatgc gctgctggaa aacgtcaccg
    34381 tgcgtgaaga tggcactatc gactttgatg atggttcaaa aaccgagaac acccgcgttt
    34441 cttatccgat ctatcacatc gataacattg ttaagccggt ttccaaagcg ggccacgcga
    34501 ctaaggttat cttcctgact gctgatgctt tcggcgtgtt gccgccggtt tctcgcctga
    34561 ctgccgatca aacccagtat cacttcctct ctggcttcac cgccaaactg gccggtactg
    34621 agcgtggcat caccgaaccg acgccaacct tctccgcttg cttcggcgcg gcgttcctgt
    34681 cgctgcaccc gactcagtac gcagaagtgc tggtgaaacg tatgcaggcg gcgggcgcgc
    34741 aggcttatct ggttaacact ggctggaacg gcactggcaa acgtatctcg attaaagata
    34801 cccgcgccat tatcgacgcc atcctcaacg gttcgctgga taatgcagaa accttcactc
    34861 tgccgatgtt taacctggcg atcccaaccg aactgccggg cgtagacacg aagattctcg
    34921 atccgcgtaa cacctacgct tctccggaac agtggcagga aaaagccgaa accctggcga
    34981 aactgtttat cgacaacttc gataaataca ccgacacccc tgcgggtgcc gcgctggtag
    35041 cggctggtcc gaaactgtaa tgatttgaag ctggagaata tctatccagt atcttataga
    35101 aagcaaaacg ggaggcacct tcgcctcccg tttatttacc cttcttttgt cgtgccctgc
    35161 gcccgcgtta ccggcactgg cagccaggcg cgaatggaaa gcccgccccg ctcgctggtg
    35221 ccaagctcca gcatcccgtt atggttatcc acgatacgct gcacaattgc cagccctaat
    35281 cccgtgccgc taatggtgcg cgcactgtcg ccgcggacaa acggctggaa caggtgctta
    35341 cgttgttccg gcgcaattcc cggaccgtca tcttccacct ggaaccaggc gcgattcggc
    35401 tccgttccgc tgctgacttt gatccagcca ttgccataac gggcggcgtt gaccaccata
    35461 ttcgccaccg cgcgtttgat cgacagcggg tgcattttca cttcaatgct gccggggtaa
    35521 agcgcggttt caatttcccg ctcatagcca ctttcggcag caatcacctc accgagtact
    35581 gcattaagat ccgccatttc catcggcatc tcctgcccgg tgcgcaggta gtcgataaac
    35641 tgctcaatga tggcgttgca ctcttcgata tctttattga tcgattctgc cagatagcca
    35701 tcctgctcgc tcatcatctc agtcgccagg cgaatacgcg tcagcggcgt gcgcaagtcg
    35761 tgacttaccc ccgccatcag cagcgtgcgg tcatccgcca gttgcttaac accagccgcc
    35821 atatggttaa aggcacgggt aacggaacgc acctccgaag cgccatattc acgcagcggc
    35881 ggcggaataa tccctttacc aacctgcaag gctgcgtgtt cgagatcgac caacggtcgg
    35941 ttctggatac gaataaacag ccacgccccg cctatcgcca atagcataat cgccagcgta
    36001 tagcggaaca gcggagagaa atcgccctga tgaatttcgg tcagcggcac gcgtacccag
    36061 atattgggcg acagccaggt tttcagccag acgacaggcg aacttttgtt gacctcaacg
    36121 cgcatttccg tcgggccgcc cagttgctgt gccatctgat ggcttaagaa ttcatagtgt
    36181 tgcgcccaac gcagacctgc ctcttcggca gcctcgttgg agtagagaga gatccccagc
    36241 tcacggtaga tctcccgacg gaaagcggga ggcacaacca actgcgtgcc gtcctccagt
    36301 tgcagtttgt cggtcatcaa catacgcact tcgtacgcga ggactttatt aaactgctgg
    36361 aggctcggca aaatcgcgaa gttcagcacc accagataag tcgtcaccag gctggcgaac
    36421 agcaaggtga cgatgagcaa taacgtacgg gcaaatgaac ttcgtggcga gaagcgcaat
    36481 cgcctcatgc tttagagccg tccggtacaa agacgtagcc caggccccaa acggtctgaa
    36541 tgtaacgcgg atgcgctgga tcttcttcta ccatgcggcg cagacgcgaa atctgcacgt
    36601 cgatggagcg ttccattgcg gaatattcac gaccacgggc aaggttcatc agcttatcgc
    36661 gggagagcgg ctcacgcgga tggctgacca gtgccttcag taccgcaaac tcaccgctgg
    36721 tgagcggcat cggctcatct tcgcggaaca tttcgcgcgt accgaggtta agtttgaact
    36781 taccgaaagc aattaccgcc tcttcctgtg acggtgcgcc tggcagttcg tttgcctgac
    36841 gacgcagcac cgcacgaata cgggccagca gttcacgcgg gttaaacggt tttggaatgt
    36901 agtcgtcagc gccaatctcc aggcctacga tacggtccac ttcttcccct ttcgccgtca
    36961 ccataatgat cggcatcggg ttgctctgac tacgaagacg tcggcaaatc gacaagccat
    37021 cttcaccagg taacattaaa tccagtacca taagatggaa agattcacga gtcagcaggc
    37081 gatccatctg ttctgcatta gcgacgcttc gaacctggaa gccttgttcg gtgagataac
    37141 gttccagcag cgcacgcagg cgcatgtcgt catcgaccac cagaatcttg tagttctctt
    37201 gcattgtttg tactcccaaa ggttcgcaac aatttgtaag cgtgtattct taaaaaagct
    37261 cacgttcgtc accagctaaa tctggtatga atttcagcct aaattgttac aaagcatatt
    37321 aaacagcagc ttaagtatac aatttattcg gcgaaacatt attgattctg ttgatatgat
    37381 cacgttatac ccaatgtgcg cattatcaaa cagacaaagg gaatcaacga gatgaaaacg
    37441 cccctggtta cccgggaagg gtatgaaaaa ctcaaacaag agcttaatta tctctggcgt
    37501 gaagaacgcc cggaggtcac aaaaaaggtg acctgggccg caagtctggg cgaccgcagc
    37561 gaaaatgctg actatcagta taataaaaag cgtctgcgtg aaatcgaccg tcgcgtgcgc
    37621 tatctcacta aatgcatgga aaatctcaaa atcgtcgatt actcccctca gcaggaaggc
    37681 aaagtctttt ttggcgcgtg ggtggagatt gaaaacgacg atggcgtgac tcaccgtttc
    37741 cgtattgtcg gctacgatga aatttttggc cgtaaagatt acatctctat cgattccccg
    37801 atggcccgcg cattgctgaa aaaagaagtc ggcgatctgg cggtggtgaa tacccctgcc
    37861 ggggaagcga gctggtatgt taatgctatc gagtacgtga aaccgtaagg aagagtctta
    37921 acctcctgcc gatggctggc atttttgcca gccagtccgt ataactatcc cctgattttt
    37981 gatccgaaaa gatgaactca aaccatgatg aatgattcgt tctgccgcat tattgcgggt
    38041 gaaattcagg cgcgcccgga acaggttgac gctgccgttc gcctgcttga cgaagggaat
    38101 accgtgccgt ttatcgcacg ttatcgtaag gaaatcaccg gcggtctgga tgacacgcag
    38161 ctgcgtaatc tggaaacgcg tctgagctat ctgcgcgagc tggaagagag acgtcaggcg
    38221 atcctcaagt ccatttccga gcaaggcaaa ctcaccgatg atctggcgaa ggccatcaac
    38281 gccaccctaa gcaaaaccga actcgaagac ctctacctgc cctacaaacc taaacgccgc
    38341 acccgcgggc aaatcgccat tgaagcaggg cttgagccgt tggctgacct gctgtggagc
    38401 gatccgtcac acacgccaga agtcgccgct gcacaatatg ttgatgccga taaaggcgtg
    38461 gcagatacca aagccgcgct ggatggcgcg cgctatatcc tgatggaacg gtttgccgaa
    38521 gatgccgcgc tgctggcgaa agtgcgtgat tatctgtgga agaacgcgca tttggtttct
    38581 acggtggtga gcggtaaaga agaggaaggg gcgaaattcc gcgactattt cgatcatcac
    38641 gaaccgttgt ccacggtgcc ttctcaccgc gcgctggcga tgttccgtgg gcgtaacgaa
    38701 ggcgtactcc agctttcgct gaatgccgat ccgcagttcg atgagccgcc caaagagagc
    38761 tattgcgagc aaatcatcat ggatcacctt ggcctgcgcc tgaacaatgc cccggcggat
    38821 agctggcgca aaggcgtagt gagctggacc tggcgcatca aggtgctgat gcatctggaa
    38881 accgaactga tgggtaccgt gcgcgaacgc gcggaagatg aagcaatcaa cgtctttgcc
    38941 cgtaacctgc acgatctgct gatggcggcc cctgccggac tgcgtgcaac gatgggcctc
    39001 gatccgggtc tgcgtactgg ggtaaaagtg gcggtggtcg atgccactgg caaactggtg
    39061 gcgaccgata ccatttaccc gcacaccgga caagccgcaa aagcagcgat gaccgttgct
    39121 gccttgtgtg aaaaacataa cgttgaactg gtggcgatcg gcaacggtac agcttcccgc
    39181 gaaactgaac gtttctatct cgacgtgcag aagcagttcc cgaaagtgac cgcacagaaa
    39241 gtgatcgtca gcgaagctgg cgcgtcggtt tactcagctt ccgagctggc agcgcaggag
    39301 ttcccggatc tcgacgtttc gctgcgtggt gcggtatcta tcgcccgccg tttgcaggat
    39361 ccgctggcgg agctggtgaa aatcgatccg aaatctatcg gcgtaggtca gtatcagcat
    39421 gacgtcagcc agacgcaact ggcccgcaaa ctggacgcag tagtagaaga ctgcgtaaac
    39481 gccgttggcg tcgatctcaa cactgcttct gttccgctgt taacccgcgt ggcgggcctg
    39541 acgcgcatga tggcgcaaaa catcgtggcc tggcgcgatg agaacggtca gttccagaac
    39601 cgtcagcaac tgctgaaagt gagccgtctg gggccgaaag ccttcgagca gtgcgcgggc
    39661 ttcttgcgca ttaaccatgg tgataacccg ctggacgcgt ctaccgtcca cccggaagcc
    39721 tatccggtgg tggaacgcat tctggcagca acacagcagg cactgaaaga tctgatgggt
    39781 aacagcagcg aactgcgtaa cctgaaagcg tctgacttta ctgatgaaaa attcggtgtg
    39841 ccgacagtaa ctgacatcat caaagagctg gaaaaaccgg gtcgcgatcc gcgtccggaa
    39901 tttaaaaccg ctcagtttgc cgatggcgtc gagacaatga acgacctgca accgggtatg
    39961 atcctcgaag gtgcggtgac caacgtcacc aactttggcg cgtttgtcga tatcggcgtg
    40021 catcaggacg gcctggttca catctcttca ttgtcgaaca agtttgtaga agatccgcat
    40081 accgtggtga aagcgggcga cattgtgaag gtgaaagtgc tggaagtgga tcttcagcgt
    40141 aaacgtatcg ccctgaccat gcgtctggac gagcagcctg gcgaaaccaa cgcccgtcgc
    40201 ggcggcggta atgaacgccc gcaaaacaac cgcccggcag ccaaaccgcg cggacgtgaa
    40261 gcgcagcctg ccggtaacag cgcgatgatg gacgcgctgg cggcagcaat gggcaaaaaa
    40321 cgttaaacgc cctgattgac ccctaaacct gcgcgataag gtgtccacgc cgcatctggc
    40381 aacgaacgcc ttgtctgaca taaaagtgcc ggagaatatc tccggcattt ttattccaca
    40441 gccaaactca caatatattc cggcaacatt tatcacttca ttaacaactg aaaccttaat
    40501 taaacattag ccagtcctgg taattcgcta ttcgaattat attttcgctg cgatataacc
    40561 ttgagccaca tcaacattga gtcagattat tattcaaacc aacattcgca cacattttaa
    40621 gtattgctga tagaaaccat tctcattatc attgtgctgt tgattattta atctctcctt
    40681 cgttggcaaa tcatctggtc tcatgtcgct gtcaaacgcc ccatgaggta gttatccagt
    40741 taatgagaaa caagtaggca cctatgcaat acactccaga tactgcgtgg aaaatcactg
    40801 gcttttccca tgaaatcagc ccggcatatc gccaaaaact gctttctctt ggcatgttac
    40861 ctggctcctc ttttaatgtg gtgcgcgtcg ctccactcgg cgaccccatt catatcgaaa
    40921 cccgtcgtgt gagcctggta ttacgcaaaa aagatctggc cttattagaa gtggaagcgg
    40981 tttcctgtta atacggtgat aacaacaatg aaaaaattaa ccataggctt aattggtaat
    41041 ccaaattctg gcaagacaac cttatttaac cagctcactg gcgcacgtca gcgtgtaggt
    41101 aactgggctg gcgttaccgt cgaacgtaaa gaagggcaat tctccaccac cgatcatcag
    41161 gtcacgctgg tggacctgcc cggcacctat tctctgacca ccatctcatc gcagacctcg
    41221 ctcgatgagc aaatcgcctg tcactacatt ttgagtggcg acgccgacct gctgattaac
    41281 gtggtggatg cgtctaacct tgagcgtaac ctgtacctga cgctacaact gctggaactc
    41341 ggcattccct gcatcgtggc actgaacatg ctcgacattg ccgagaagca aaatattcgt
    41401 attgaaattg atgctctgtc ggcgcgtctt ggctgcccag tgatcccgct ggtttcaacc
    41461 cgtggtcgcg gtattgaagc gctcaagctg gcgattgatc gctataaagc taacgagaat
    41521 gtggaactgg tgcattacgc acagccgcta ctcaacgaag cagattcact ggcaaaagtg
    41581 atgccttccg acatcccgct gaaacaacgt cgctggctgg gcctgcaaat gctggaaggc
    41641 gatatctaca gccgcgccta cgccggtgaa gcgtcgcagc atctggatgc cgccctcgcc
    41701 cgtctgcgta atgagatgga cgatccggcg ctgcacattg ccgatgcgcg ttaccagtgc
    41761 attgctgcca tctgtgatgt ggtaagcaac accctgacgg cagaacccag ccgtttcacc
    41821 actgcggtag ataaaatcgt gctcaaccgt ttcctcggtc tgccgatttt cctctttgtg
    41881 atgtacctga tgttcctgct ggctatcaac atcggcgggg cgttacagcc gctgtttgac
    41941 gtcggctccg tggcgctatt tgtgcatggt attcaatgga ttggctacac gctccacttc
    42001 ccggactggc tgactatctt cctcgcccag ggcctgggtg gcggcattaa caccgtgctg
    42061 ccactggtgc cgcagattgg catgatgtac ctgttcctct ccttccttga ggactccggg
    42121 tatatggcgc gtgcggcgtt tgtgatggac cgtctgatgc aggcgctggg cttgccgggg
    42181 aaatcctttg tgccgctgat cgtcggtttc ggttgtaacg taccgtcggt aatgggtgca
    42241 cgtacgcttg atgcaccgcg tgaacgtctg atgaccatca tgatggcacc gtttatgtcc
    42301 tgcggcgcgc gtctggctat cttcgcagta ttcgcggctg ccttcttcgg gcagaacggt
    42361 gcgctggcgg tcttctcgct gtatatgctg ggtattgtga tggcggtgct gactggcctg
    42421 atgctcaagt acaccatcat gcgcggtgaa gcgacgccgt ttgtcatgga gttgccggtc
    42481 tatcatgtac cacacgttaa aagcctgatt atccagacct ggcagcgtct gaaaggcttc
    42541 gttctgcgtg ctggtaaagt gatcatcatc gtcagcattt tcctgagcgc tttcaacagc
    42601 ttctcgctga gcgggaaaat cgtcgataac atcaacgact cggcgctggc gtccgtcagc
    42661 cgggtgatca ccccggtctt caagccaatt ggcgtgcatg aagataactg gcaggcaacg
    42721 gttggcctgt ttacaggtgc catggcgaaa gaagtggtag tgggtacgct caacaccctc
    42781 tacaccgcag aaaatattca ggacgaagag ttcaatccgg cagaatttaa cctcggtgaa
    42841 gagctgttca gtgcgataga tgaaacctgg cagagcctga aagacacctt cagccttagc
    42901 gtactgatga accccattga agccagcaaa ggcgacggcg aaatgggtac cggggcgatg
    42961 ggcgtgatgg atcagaaatt cggtagcgca gcagcagctt acagctacct gattttcgtc
    43021 ctgctgtatg taccatgtat ctcggtgatg ggagccatcg cccgtgaatc aagccgtggc
    43081 tggatgggct tctccatcct gtgggggctg aatatcgctt actcgctggc aacattgttc
    43141 tatcaggtcg ccagctacag tcagcaccca acttacagcc tggtgtgcat tctggcggtt
    43201 atcctgttta acatcgtggt tatcggtctg ctgcgccgcg cgcgtagccg ggtggatatc
    43261 gaactgctgg caacccgcaa gtcggtaagc agttgctgcg cagccagcac caccggtgat
    43321 tgccattaat ggcttcactt attcaggtgc gcgatttgct ggcgttacgg ggccgtatgg
    43381 aagcggccca gataagccag acattgaaca ctccacagcc aatgattaac gccatgctgc
    43441 aacaactgga aagtatgggc aaagccgtgc ggattcagga agaacctgac ggctgcctct
    43501 ctggcagttg taaaagctgc ccggaaggaa aagcctgtct gcgcgagtgg tgggcgctgc
    43561 gttaacctta ctccatcgcc tgttttggaa agcccggtat gcgtctgcat ccgggctttt
    43621 ttgcgtgcgg ctttccataa aaatgcaact cttgcagcac ggcgtaagtt cctttgaaag
    43681 catctcgcag ggatgaaaac tcgctaatgc acagatgtgg agtggcgcgt agagtcgcgg
    43741 cattcaaaca acaggtgaag gaacgccatg agtaaaaagc agagttccac cccacatgat
    43801 gcgctgttca aactcttttt acgccaaccg gacacggctc gcgattttct tgcgttccat
    43861 ttaccggccc ccattcacgc gctctgtgat atgaaaaccc tcaagctgga gtcgagcagc
    43921 tttattgatg acgatctgcg tgaaagctat tccgatgtgc tgtggtcggt gaaaacggaa
    43981 caaggaccag gatacatcta ttgtctgatt gaacatcaaa gcacctcaaa caaactgatc
    44041 gcatttcgca tgatgcgtta cgctattgcc gcaatgcaaa atcaccttga tgctggatac
    44101 aaaacgttgc cgatggtggt gctattgttg ttttaccacg gtattgaaag cccctatccc
    44161 tattcgctgt gttggctgga ttgtttcgcc gatcccaaac tggcaaggca gctttatgcc
    44221 tccgcatttc cgctgattga tgtcaccgtc atgcctgatg atgaaatcat gcagcaccga
    44281 cgtatggcgc tgctggagtt aattcaaaaa catattcgtc aacgcgacct gatggggctg
    44341 gtagagcaaa tggcctgctt attaagtagt ggatacgcta atgacagaca aatcaaaggg
    44401 ctgtttaatt acatactgca aaccggcgac gcggtacgtt ttaacgattt tatcgacggc
    44461 gttgccgaac gttcaccgaa acacaaggag agtttaatga caattgcgga aagattgcgg
    44521 caggaggggg aacaatccaa agccctgcat atagccaaaa taatgcttga atccggagtc
    44581 cctcttacag acatcatgcg ctttaccggg gtgtcagaag aagagttggc tgcggcgagc
    44641 cagtaaattt ctgtctcgcc atttcaaaag ttgcctacac cctctgcttc aacgccacca
    44701 gcaggtgaca aaactcgacc ggatgcgaaa taaacggcgc atgggccgct ttggcgaaga
    44761 tatatgattc gctgtgaggc caaagtttat ccagcatcgg caccactttg cgcggcacca
    44821 gaccgtcgag atagccatac aatcgcaaaa acggcatggg cacgttttgc agcggctgac
    44881 ggagatcgac cgttttcagg atttccagcc cgccattaag cacgtcaacc tccggcatcg
    44941 gtaacgccag aacggttttc ttcaacgccc gcgcatcctg gcgcgccgtt tcagtcccca
    45001 tggtttgtaa cgccaggaac cgctccactg tacgctgaaa atcatcactg agttgctgct
    45061 gaaatcccgc cagcacgtcc ggttttatcc ccggccactc gtcacgagca ctaaaacaag
    45121 gtgacgacgc cacggtgacc agcgcctgaa cacgctcggg atgggttaac gcaatctggc
    45181 ttgccaccag cccgcccaga ctccagccta accaaatggc tttatcaggt gcctgttgca
    45241 gcacggcttc ggccatatca gcaagtgaca gcgcaccaaa tccccggcta cgcccgaagc
    45301 cgggcaggtc aacaaggtgc agcgtaaaat gcgagctaag ttcctcgtca atgcaacgcc
    45361 acacttcggc attcagtccc catccgtgca gcagcacaag atgaacattc ccctgacctt
    45421 tggtctgcca ccagatgtta ttcatccgct attgttctct tttgacttac aaggatgaac
    45481 atatgctaac agtaccggga ttatgctggc tatgccgaat gccactggcg ttaggtcatt
    45541 gggggatttg ttcggtctgc tcacgcgctg cccgtacaga taaaacgtta tgtccacaat
    45601 gtggattacc cgccacacac tcacatcttc cctgcggtcg ctgcctgcaa aaaccaccgc
    45661 cctggcaaag actggtcacg gttgccgact atgcgccgcc gttaagtccg cttatccacc
    45721 agcttaaatt ttcccggcgc agtgaaatcg ccagcgccct gtcacgtctg ttactattgg
    45781 aagtcttaca cgctcgtcgc accaccgggt tgcaattgcc ggatcgcatc atcagcgttc
    45841 cgttatggca gcggcgtcac tggcgtcggg gatttaatca gagcgatttg ctgtgtcagc
    45901 cgttatcacg ctggttgcac tgccaatggg atagcgaagc cgtcacacgt acacgggcca
    45961 ctgcgaccca gcattttctc agtgcccggc tgcgcaagcg caacctgaaa aatgcctttc
    46021 gtcttgaatt gcccgtgcaa ggtcgccata tggtgattgt ggatgatgtc gttaccaccg
    46081 gaagtaccgt cgcagagatt gcgcagttgc ttttacgcaa tggtgcggcg actgtccagg
    46141 tctggtgcct ttgtcgaacc ttgtagagcc tcgatgatgg gcgtattata accaactaaa
    46201 atagtcaact attaggccat tactatgatc cgtatttccg atgctgcaca agcgcacttt
    46261 gccaaactgc tggcaaatca ggaagaaggg acacaaatcc gcgtatttgt gattaaccct
    46321 ggcacgccta acgctgaatg tggtgtttct tattgtccgc cggatgccgt ggaagccacc
    46381 gacactgccc tgaaatttga cctgctgacc gcgtatgttg atgagttaag cgcaccatac
    46441 ctggaagatg cagagatcga ctttgttacc gaccagttgg gttctcagtt aacgctgaaa
    46501 gccccgaacg ccaaaatgcg taaagtggca gacgatgcac cgctgatgga gcgcgtggag
    46561 tatatgctgc agtcgcagat caacccacag cttgctggtc acggtggtcg cgtttcgctg
    46621 atggaaatca cagaagacgg ttacgccatt ctgcaatttg gcggcggctg taacggttgt
    46681 tccatggtcg atgtgacgct gaaagaaggg atcgagaagc agctgctgaa cgaattcccg
    46741 gagctgaaag gtgtgcgcga tctcaccgaa caccagcgcg gcgaacactc ctactactaa
    46801 gttatcccct cttttgtaga ttgcccgatg cgacgctaaa gcgtcatttc gggcctacgt
    46861 tgaacatgtg ccagacgttg gcacagcatt gctttggcaa atccctccct tcgtttcccc
    46921 gcgataatat gaccaacctc tcataattta aatttacccc gctctggtga ttctcaaacg
    46981 ccagatgtta cccgtatcat tcacatgggt accaaacata ctcctgacat ctgactacaa
    47041 taattagttt tagtgggtat cagtcgtggt gccgcaatat ctctgttccc gattgggata
    47101 attagagttt gtcgtcagaa aattgacgtt acccataaca aatgaaaggc caggtaaatc
    47161 atgccattag tcattgttgc tatcggtgta atcttgttgt tgctcctgat gatccgcttc
    47221 aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg ctgttggatt aatgcaagga
    47281 atgccgctgg ataaagttat tggctccatc aaagccggtg tcggcgggac gctcggtagc
    47341 cttgccctga tcatgggttt tggcgcaatg ctgggcaaaa tgctggcaga ctgcggtggc
    47401 gcacaacgta tcgccaccac gctgattgcc aaatttggta aaaaacacat ccagtgggcg
    47461 gtggtactga ccggttttac cgttggtttt gccctgttct atgaagtggg ctttgtgctg
    47521 atgctgccgc tggtgtttac catcgcggct tctgcgaata ttccactgtt gtatgttggt
    47581 gtaccaatgg cagctgcact gtctgtgacc cacggcttcc tgccaccgca tccgggtccg
    47641 actgcgattg ccaccatttt caatgccgat atgggtaaaa ccctgctgta cggtactatt
    47701 ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg ctcgcgtgtt gaaaggtatc
    47761 gataagccaa ttccggaagg tctctacagc gcgaaaacct tcagcgaaga agagatgccg
    47821 agctttggcg tcagcgtctg gacttctctg gtgccggttg tgctgatggc gatgcgtgcg
    47881 attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc cggtagcgga gttcctcggt
    47941 gacccggtaa tggcaacgct gattgccgtg ctgattgcga tgttcacctt tggtctgaac
    48001 cgtggtcgtt caatggatca gattaacgac acgctggttt cttccatcaa aatcattgcg
    48061 atgatgctgt tgatcatcgg tggtggcggt gcgttcaagc aggtgctggt agacagcggc
    48121 gtggacaaat acattgcttc catgatgcac gaaaccaaca tttctccgct gctgatggcc
    48181 tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg caaccgttgc ggcaatcact
    48241 gcgggtggta tcgcggcacc gctgattgca acgacgggtg ttagcccaga gctgatggtt
    48301 attgcggtgg gttccggtag tgtgattttc tctcatgtga acgatccggg cttctggctg
    48361 ttcaaagagt actttaacct gactatcggc gagaccatca aatcctggtc gatgctggaa
    48421 accattatct cggtgtgcgg tctggtaggc tgtctgctgc tgaatatggt gatttgataa
    48481 cactgactgc cggatgcatc gcatccggca tcaaccgcac tctacttctt cttcgctgca
    48541 gctctgcgcc gtctgtccaa atccttcagc aacttgttca cgccatcatc ggcaaacatc
    48601 gtctcaagcg ttgcggaaag cttgcgtcgc cagtttttat actggtaact ggtgccagga
    48661 atattcaccg gttcggccat atccagccag tcttccggct gtagtcctaa cagagcactg
    48721 ttactgtcgg caatgtagcg ctgcaaacca cggttcagcg tcggcgtcat cgacatcaac
    48781 gatgccttat gcccggcacg ttttggcaga caaccatatt tatgcagtgc atccagcagc
    48841 ccttgcttcg ccagttcgcg atcctgatac agaccgcgca gtaccacttc atccggatac
    48901 agccccaggg ttttgcccag cgttagatcc ccgctctccc agtaaccgcg tagcgtaggc
    48961 aggtcatgtg tcgccgcaac cgccatcgac tgctccggat acgctttcgg tgcacggaac
    49021 gtcttctcgt ggtcgttttc gaaatagagc actttgtagg agtacacacc gctgctgcgc
    49081 agcttaccga caatctctac cggtacggta ccgagatctt caccaatcac catacagcga
    49141 tgacgtttac tttcgagtgc cagaatcgag agcagatcat ccaccggata gtgaacatac
    49201 gcgccctgat ctgccgtctc gccatacggt atccaccaca aacgcagcat cgacatcaca
    49261 tggtcaattc gtaatgcgcc gcagttttgc atattggcac gcaacagctc gataaacggt
    49321 tcataggcac gcgcggtgat gatatgcggg tccattggcg gtaatcccca gttctgcccc
    49381 aacgggccga ggatatccgg cggcgcgcca accgacgctt tcaggcaata tagttcacgg
    49441 tcacaccagg tttccgcccc accttccgct acgccaaccg ccagatcacg atacaagcca
    49501 atcggcattt catagccctg gcttatctcc cagcaggcgg caaactggct gtaagccagc
    49561 cactgcaacc agagataaaa atcgacgtca ttacgatgtt cttcgcagaa ctgacgcact
    49621 tctggtgaat ccacgttctg atacatctct ggccatgcag gccagcccca gcgcatttcg
    49681 tcctctttca cttgctgggc atgtagcgca tcaaaggctg cctgccagaa caggctgtcg
    49741 ccctgctctg caacaaactg gcgaaacgcg gtcatctgct catcatcacg ttgcgcgaaa
    49801 cctttccacg ccattcgtaa tgctgtcatt tttagggcgg taaccgtgga gtaatcgacc
    49861 cagtcggcat cgcgcgcctg ttgcagcgtc tgttgcgtgg tcggcaactg ccaccaggcc
    49921 tgagcctctt cgctaagatg gaaatcttca acggcgttaa cgtcgatata aatcacattc
    49981 agccaacggc gagaagacgg gctgtatggg ctggcactct ccggatttgc cggatagagc
    50041 gcatgaatcg ggttcaggcc gatgaacgac ccgccacgtt ttgccacatc caccagcatc
    50101 gctttgagat cgccaaaatc cccaataccc cagttttttt ccgatcgcag cgtataaagc
    50161 tgaacgcagg caccccacag cttttgttta ttcagcaacg cctgcggttc gtaacagcgt
    50221 ttcggggcga caatcacccg gcaatgcgcg cgctggtcgt cctgggtgag tgtcagcgtg
    50281 tgataacctt ccggcagctt cgtcggtaga ttgaacgctt tgccccccgt tacatggcct
    50341 ttgtactgtg ttccttcttc ggtggtcagc agccagctat attcgccgct gccctccacc
    50401 accatcggca tttttttgcc gctggtataa accatgacat tcgggactgg cgttaccgcc
    50461 actttcgtgg cggtacgttg atgcatcgcg tcaagcaaac gccgtttggt ttcggcgcta
    50521 atcgactgcg gtttaccgtg ggcattgatg taattggggc taatccccgc cgccagcgcg
    50581 gcattatcca gacgtttgct ttccatcgag cttccttagc gttttgcctg ccagatacga
    50641 gcctgataat cgcgaataga gcgatccgag ctaaacatac cgcagcgggc ggtattgagg
    50701 atcgccgcgc gagtccaggc ctcctggtcg cggtacagca catccacctg cttttgtgcc
    50761 tcaacatagg ctgcgaaatc cgccatcacc agatacggat cgccgccttg tttgccgata
    50821 ctgtgcagca tctggtcgaa ggcatgctta tcgccgtcgc tgtatttacc gctttccagc
    50881 tctttcaata ctgcgtccag caccttatct ttcttccgcc atttcaccgg gtcgtagcct
    50941 ttggccagaa tagccttcac ttgttccacg gtatgaccaa aaataaagat attttcttca
    51001 ccgactttct cggcgatttc aacgttcgca ccatccagcg taccgacagt aagcgcacca
    51061 ttgagcgcca gtttcatatt gccggtaccg gaagcttctt tacctgcagt cgaaatttgt
    51121 tcggagatat ccgccgccgg gatcagtttt tccgccgccg aaacgcaata atccggcagg
    51181 aacaccacct tcaacttatc gccaaccagt ggatcgttgt tgatcacgtc agccactttg
    51241 ttgatcgcaa agataatatt cttcgccagg tagtagcccg gtgccgcttt cgcgccgaag
    51301 aggaagacgc gcggtacgcg atcagcctgc gggttttcac gaatttcttt gtacaacgcc
    51361 agaatatgca gcagattcag gtgctggcgt ttgtactcat gcagacgttt gatctgaata
    51421 tcgaaaatcg cctgtggatt gatctcaata ccggtacgaa ctttcacaaa ctccgccaga
    51481 cggactttat tcgcctgctt gatctcgcga tattgctgac ggaatttcgc atcatcagcg
    51541 aatttttcca gattgatcag ctgatcgaga tcgttagccc actctttttg cagtgattta
    51601 tccaacagag ccgccagtgc agggttgcac tgtttgatcc agcgacgtgg ggtaataccg
    51661 ttggtgacgt tatggaattt gttcggccat agctggtgat attccgggaa cagatctttc
    51721 accaccagat ccgagtgcag cgccgcaaca ccgttcaccg cgaaaccgcc aaccacacac
    51781 aggttcgcca tatgcacttg tttatcgtgc accaccgcca gtttggccca cactttttca
    51841 tcgcccggcc aggttttctc caccagcgtt ttaaagcgag tattaatttc gttaataatc
    51901 tgcatatggc gcggcagtaa gcctttcacc agtttcacat cccagcgttc cagcgcttct
    51961 ggcatcaggg tatggttggt gtaagcgaaa gttttgctag taatggccca ggcgtcatcc
    52021 cagctcatct ggtgctcatc gatcagcacg cgcagcagtt ctggaatcgc gatagttggg
    52081 tgggtatcgt tcagctgaat aacttcgtaa tccgccagtt cgtgcagttt acgccccgcc
    52141 agatgatggc gacgcaaaat atccgctacc gaacaggcac actggaagta ctgctgcatc
    52201 aagcgcagct ttttaccggc agtatggttg tcgtttggat agagaacttt ggtcagtttt
    52261 tccgcattga tgccctgctg ttcggcacgc aggaaatcac cgtcgttaaa tttagtcaga
    52321 tcaaacggat gcgcgtgcgt cgcctgccac agacgcagcg gctgcgccac gccattacga
    52381 tagccgacaa cggggagatc ccacgcttga ccggtaatgg taaactccgg ctcccagcgt
    52441 ccgtctttcg tcactttacc gccaatccct acctgcacat ccagtgcttc gttgtggcgg
    52501 aaccacgggt agttactgcg atgccagtca tccggcgctt caacctgttt gccatcgaca
    52561 aaagactggc ggaacaaacc atattgatag ttcagaccgt aacccgtcgc agactgaccg
    52621 acagttgcca ttgagtcgag gaagcacgcc gccagacgtc ccagaccacc gttacccagc
    52681 gccgggtcga tctcttcttc cagcaggtcc gtcagattga tgtcataagc cttcaacgaa
    52741 tcctgtacat cctgatacca gccgagattc aacaggttgt tgcccgtcag gcgaccaatc
    52801 aaaaactcca ttgagatgta gttaacatgt cgctgattcg ccaccggctt ggcgaatggc
    52861 tgagcacgca gcatttcggc cagtgcttca ctcactgcta gccaccactg gcgaggagtc
    52921 atttcagccg cagaatttaa gccataacgc tgccactgac gtgaaagcgc ttcctgaaat
    52981 tgcttatcgt taaaaatagg ttgtgacata ggagttccac ttttcttaga ttttcaacac
    53041 aacgttatcg ctagtttgcc aggctcgatg ttgaccttcc tcatcctgcg ggggattagg
    53101 cagggaggag ttgcggggat gagcaaggaa atgtgatctc aaccacttaa agctagtgca
    53161 aaccacagga ttagcagcaa atcaatgcaa tacagcgcag aaaatctgta tctaagtgca
    53221 aaaaatggcc gttgcgtatt ttcaaaaagc ggaaggtaac tctataaatt aagtaaagga
    53281 gtgaaacagt ttcataagta aaatatccag tgtgctccat ctcattctta atagatttat
    53341 taagatcatc tttttagatg gcactttcat caggaatgaa gaagaaaccc ttgcttaaat
    53401 gaatctgatg aacataaggg aaaccagtat tcacgctgga tcagcgtcgt tttaggtgag
    53461 ttgttaataa agatttggaa ttgtgacaca gtgcaaattc agacacataa aaaaacgtca
    53521 tcgcttgcat tagaaaggtt tctggccgac cttataacca ttaattacga agcgcaaaaa
    53581 aataatattt cctcattttc cacagtgaag tgattaacta tgctgattcc gtcaaaacta
    53641 agtcgtccgg ttcgactcga ccataccgtg gttcgtgagc gcctgctggc taaactttcc
    53701 ggcgcgaaca acttccggct ggcgctgatc acgagtcctg cgggctacgg aaagaccacc
    53761 ctcatttccc agtgggcggc aggcaaaaac gatatcggct ggtactcgct ggatgaaggt
    53821 gataaccagc aagagcgttt cgccagctat ctcattgccg ccgtgcagca ggcaaccaac
    53881 ggtcactgtg cgatatgtga gacgatggcg caaaaacggc aatatgccag cctgacgtca
    53941 ctcttcgccc agcttttcat tgagctggcg gaatggcata gcccacttta tctggtcatc
    54001 gatgactatc atctgatcac taatccagtg atccacgagt caatgcgctt ctttattcgc
    54061 catcaaccag aaaatctcac cctggtggtg ttgtcacgca accttccgca actgggcatt
    54121 gccaatctgc gtgttcgtga ccaactgctg gaaattggca gtcagcaact ggcatttacc
    54181 catcaggaag cgaagcagtt ttttgattgc cgtctgtcat cgccgattga agccgcagaa
    54241 agcagtcgga tttgcgatga cgtttccggt tgggcgacgg cactacagct aatcgccctc
    54301 tccgcccggc agaataccca ctcagcccat aagtcggcac gccgcctggc gggaatcaat
    54361 gccagccatc tttcggatta tctggtcgat gaggttttgg ataacgtcga tctcgcaacg
    54421 cgccattttc tgttgaaaag cgccattttg cgctcaatga acgatgccct catcacccgt
    54481 gtgaccggcg aagaaaacgg gcaaatgcgt ctcgaagaga ttgagcgtca ggggctgttt
    54541 ttacagcgga tggatgatac cggcgagtgg ttctgctatc acccgctgtt tggcaacttc
    54601 ctgcgccagc gctgccagtg ggaactggcg gcggagctgc cggaaatcca ccgtgccgcc
    54661 gcagaaagct ggatggccca gggatttccc agcgaagcga ttcatcatgc gctggcggca
    54721 ggcgatgcgc tgatgctgcg cgatattctg cttaatcacg cctggagttt gttcaaccat
    54781 agcgaactgt cgctgctgga agagtcgctt aaggccctgc cgtgggacag tttgctggaa
    54841 aatccgcagt tagtgttatt gcaggcgtgg ctaatgcaaa gccaacatcg ctatggcgaa
    54901 gttaacaccc tgctagcccg tgctgaacat gaaatcaagg acatcagaga aggcaccatg
    54961 cacgcagaat ttaacgctct gcgcgcccag gtggcgatta acgatggtaa tccggatgaa
    55021 gcggaacggc tggcaaaact ggcactggaa gagctgccgc cgggctggtt ctatagccgc
    55081 attgtggcaa cctcggtgct gggtgaagtg ctgcactgca aaggcgaatt gacccgctca
    55141 ctggcgctaa tgcagcaaac cgaacagatg gcacgccagc acgacgtctg gcactacgcc
    55201 ttatggagtt taatccagca aagtgaaatt ctgtttgccc aagggttcct gcaaaccgcg
    55261 tgggaaacac aggaaaaagc gttccagctg atcaacgagc agcatctgga acagctgccg
    55321 atgcatgagt ttctggtgcg catccgagca caactgttat gggcctgggc acggctggat
    55381 gaagccgaag cgtcggcgcg tagcgggatt gaagtcttgt cgtcttatca gccacagcaa
    55441 cagcttcagt gcctggcaat gttgattcaa tgctcgctgg cccgtggtga tttagataac
    55501 gcccgtagcc agctgaaccg tctggaaaac ctgctgggga atggcaaata tcacagcgac
    55561 tggatctcta acgccaacaa agtccgggtg atttactggc aaatgaccgg cgataaagcc
    55621 gccgctgcca actggttgcg tcatacggct aaaccggagt ttgcgaacaa ccacttcctg
    55681 caaggtcaat ggcgcaacat tgcccgcgca caaatcttgc tgggcgagtt tgaacctgcg
    55741 gaaattgttc tcgaagaact caatgaaaat gcccgcagtc tgcggttgat gagcgatctc
    55801 aaccgtaacc tgttgctgct taatcaactg tactggcagg ccggacgtaa aagtgacgcc
    55861 cagcgcgtgt tgctggacgc attaaaactg gcgaatcgca ccggatttat cagccatttt
    55921 gtcatcgaag gcgaagcgat ggcgcaacaa ctgcgccagc tgattcaact caatacgctg
    55981 ccggaactgg aacagcatcg cgcacagcgt attctgcgag aaatcaatca acatcatcgg
    56041 cataaattcg cccatttcga tgagaatttc gttgaacgtc tgctaaatca tccggaagtg
    56101 ccagaactga tccgcaccag cccgttgacc caacgtgaat ggcaggtcct ggggctgatt
    56161 tactccggtt acagcaatga acaaattgcg ggcgaactgg aagtggcggc aaccaccatc
    56221 aagacgcata tccgcaatct gtaccaaaaa ctcggcgtcg cccaccgtca ggctgccgtg
    56281 cagcacgcgc agaaactgct gaagatgatg ggttatgggg tgtgagttta gccggataat
    56341 gcattggatc cggtttacat ctttacatca ttcaatactc acccgcgtta cgccatctgt
    56401 ttctatcaaa ccaaaccgca ccggcaagaa acgctccacc accgcgatat tggtcagcag
    56461 atggcatgaa ggatgggcga ccttaaattc tcccgcgccc gccagcgcca tcggcagtac
    56521 cagttggtcg gcgagatatt cccccaccgc cgctgggctt gccaggtagc gtttcacctc
    56581 tttcactaac tgcgcggcta ccacctcggc actgacgcgc ttttcaccga cgacgaaaaa
    56641 gcgttcagtg atattttcac tttcgacttc cagcgagacg gtattacccg gcccctggtc
    56701 gcgcggcagg ttatgaatat tctgttcatg cagggaaaaa ctccccgcca gtgtagcgat
    56761 ttcacgctca gcaacatggc gcggcacacc agctaataga acttctccac gcatctgcac
    56821 aatgttcccg cgctcgccaa gttgcaaggt gttaaacgat gccacgggcg agacttccgt
    56881 tgccaccacg ccgcctccgg caggataaaa accgtgacgt aacagcgtgg tttgctgatg
    56941 aattcctatt ttcgccagca gcggctccag cacccggcgg ataaaatccg caggcggggc
    57001 cgacgggtta tcggtgcctc cgctcacttc aacacgcgaa ggtccatcgg caaaccacag
    57061 cgcgggcagc accgtttgca gcaccagcgt acagcttccg gcactaccga tagcaaagcg
    57121 gtactcgccg ccgcgcacgg tgccaggccg gaagaccaga cgctgcgacc ccagctccgc
    57181 gccttccacc gttgccctac aaatttccgc agccgctttt accgcggtca gatgctggcg
    57241 caacagcccc ggtttcgctc gcccggcacg aatgctggtg atggtaaatg gctggccggt
    57301 tatcatcgac aggctcagcg ccgagcgcag gatctgcccg ccaccttcgc cctgtgcgcc
    57361 atccagcgca atcatccttt tcatcattta tccttttacg cacaccacct gacgcagggt
    57421 atagataact tccaccagat cgctttgtgc cgccatcacc gcatcaatat ctttatacgc
    57481 catcgggatt tcgtcgatca cttcggcatc tttacggcat tccacatgcg cggtggcacg
    57541 aatttgatct tccacgctga acagtttttt cgctttagta cggctcatta cccgcccggc
    57601 accgtggctg cacgaacaga acgactcttc atttcccagc ccacggacga taaagctttt
    57661 cgctcccatc gaaccgggaa taattccata ttgaccagca cgcgcagaca ccgcgccttt
    57721 acgcgtcacg tagatctctt cgccaaagtg ctgttctttt tgcacatagt tgtggtgaca
    57781 gttgatctct tccatcgcca gcgtttgctg ttgtcttacc gttttctgcg taacgctctg
    57841 caacgctgtt actacgtttt ccatcattgc atcgcggtta aggctggcaa aaagctgcgc
    57901 ccaggccacc gctttcaggt aatcatcaaa gtattccgta ccttccataa agtacgccag
    57961 atcacgcgac ggcaacgttt caagctggtc ctgcatctct ttttgcgcca gatcgataaa
    58021 gtaagtcccg atggcgttac caattccgcg tgaaccggag tgcagcataa tccacacctg
    58081 atccgactca tcaagacaga tttcaataaa atggttaccc gttcccagcg ttcccagatg
    58141 tttatagtta ttggtattca ggaaacgcgg atatttttgc gttaaccact gataaccggc
    58201 ttccagctca gcccatttag catcgacgtt aacaggtgga ttttcccagg cacctttatc
    58261 acgtttacaa cggccagtgg tacgcccgtg cggcacggcc gtttcaatcg cctgacgcag
    58321 ctctgccagg ttttcaggca ggtcttccgc cgttaacgcg gtacgcagcg cgttcattcc
    58381 acagccaata tccacgccca ccgccgccgg aataatcgcc cctttggtcg ggatcacgct
    58441 accaatggtg gaacctttac ccaggtgtac atcaggcatt accgcaatat gtttgaaaat
    58501 aaacggcatc ttcgccgtat taataagttg ctgacgcgca tcggcctcta ccggcacgcc
    58561 tttggtccac atttttaccg gggcattttc agtggtcagt aattcgtaat tcattttgtt
    58621 tttctctttt cgttgtttgc tgtcctgata attgcaaccg tcgtgccaga aaattaaata
    58681 agcagcttaa ttttttaatt cattgtttta aaaaagatta tatctttacg tccgtaaccg
    58741 gagatttccc gcaaagccaa tttaccgata atgaaatatc gtcttttata aggatatcta
    58801 agatgcgtaa aacagtggct tttggctttg tcggtaccgt actggattat gccgggcgcg
    58861 gcagtcagcg ctggtcaaaa tggcgtccga cactctgttt atgccagcaa gaatcgttgg
    58921 tcatcgatcg actggaattg ttgcacgacg cccgctcgcg ctcgctattt gaaacgctta
    58981 aacgcgatat cgccagcgtt tcgccagaaa cagaagtggt gagcgttgag attgaactgc
    59041 ataacccgtg ggatttcgaa gaggtctacg cctgcctgca tgatttcgcc cgtggttacg
    59101 agtttcagcc agaaaaagaa gactatttaa ttcacatcac caccggtacc cacgtcgcgc
    59161 agatttgctg gtttctgctg gcagaagcgc gttacctgcc cgcccggctg atacaatctt
    59221 cacctccgcg caaaaaagaa cagccgcgcg gcgcaggtga agtgacgatt atcgatctcg
    59281 atttaagccg ttataacgcc atcgccagcc gctttgccga ggaacgccag caaacgcttg
    59341 attttcttaa gtccggcatt gccacgcgta acccccactt caaccgcatg attgagcaga
    59401 tcgaaaaagt ggcgatcaaa tcccgcgcgc cgattctgct taacggtcca accggcgcgg
    59461 gcaagtcatt tctggcgcga cgcatcttcg agttaaaaca ggcgcggcat cagtttagcg
    59521 gcgcgtttgt ggaagtgaac tgcgccaccc tgcgcggcga taccgccatg tcgacgctgt
    59581 ttggtcatgt aaaaggcgcg tttaccgggg cgcgggaatc tcgtgaaggt ttattacgca
    59641 gcgccaacgg cggaatgttg tttcttgatg agattggcga actgggcgca gacgaacaag
    59701 caatgctgct aaaagccatt gaagagaaaa ccttttaccc gtttggcagc gatcgccagg
    59761 tgagcagtga ttttcagctt atcgccggaa cggtgcgcga tttgcgccag ctggttgccg
    59821 aaggcaaatt tcgcgaagat ctgtacgcgc ggatcaatct ctggaccttc accctgccgg
    59881 gtctacgcca gcgccaggaa gatattgaac cgaaccttga ttatgaagtg gagcgccacg
    59941 ccacactcac tggcgacagc gtgcgtttta acaccgaagc gcggcgcgcc tggttggctt
    60001 ttgcgacctc tccccaggca acatggcgcg gtaactttcg cgaactttct gccagcgtca
    60061 cgcggatggc cacctttgcc actagcggac gcatcactct ggacgtggtt gaagatgaga
    60121 taaaccgtct gcgctataac tggcaggaga gccgcccctc cgcgcttacg gcgttgctgg
    60181 gcgcagaggc agaaaacatc gatctcttcg accgtatgca actggaacac gtgatagctc
    60241 tctgtcgcca ggcaaagtcg ctttccgctg ccggacgcct gctttttgat gtttcgcgcc
    60301 agggcaaagc cagcgtcaat gacgcggatc ggctacgcaa atacctggcg cgttttggtc
    60361 tgacgtggga agccgtgcag gatcagcaca gctccagttg aatatgatgg tccgtcagca
    60421 cctgcattac gcttactggc ggcggggcgt cggtgtagac ggcatctacc atgctgatgc
    60481 tgcccatatt gaccatcgcg ttacggccaa atttcgagtg atcgacaacc agcataacgt
    60541 ggcgcgagtt ctcaataatg gcgcgcttgg tgcgaacttc gtggtaatcg aactccagca
    60601 gcgagccgtc gctatcgatg ccgcttatcc ccagaatgcc gaaatcaagg cggaactggg
    60661 agataaaatc gagcgtcgct tcgccaatga tcccgccatc gcggctgcgt aattcgccac
    60721 cggcgagaat aatgcggaaa tcttctttta ccatcaacgt gttagcaaca ttgagattgt
    60781 tggtgacaat acgcaaattg ctgtgattaa gcagcgcgtg cgccaccgct tccggcgtgg
    60841 tgccgatatc gataaacagt gtcgagccgt tggggatttg ctccgccact ttgcgggcaa
    60901 tcctctcttt ttcttcggtc tgggtggctt tgcgatcgtg ccacggcgtg ttaaccgaac
    60961 tggaaggcag cgccgcaccg ccatggtgac gcaggatcag gttttgctcc gccagctcat
    61021 tgaggtcgcg gcgaatagtc tgcgggctga cggagaaatg ctctaccagc tcttcagtac
    61081 tgacataacc ctgctgttta accagttcga taataccgtt gtgacgttgt gtttgtttca
    61141 tttataaatc cctggaatta ttttcgtttt cgcgcattga gcgaatcaac aaaagccatc
    61201 gctaaaccca cggctaaccc ggcgatgtgt gctccgttcg ccatcgacat cccaaacaaa
    61261 tcaaaccatc cggcgacaat ccagatcagc gcaaagataa ttaacccacg ttgcaggtaa
    61321 atgccacttt gcggatcgcg ttcaccacgt agccagacgt agcccatcag cgcatacacc
    61381 acgccagaaa gcccgccaaa ccacggcccg ctgaattttt gctgcacata gccgcttaac
    61441 agggcgctaa tgagagtaat aacaattagc ttaccgctac cgaggcgttt ttccaccgca
    61501 ccgccgagat accaccacca gagcaggtta aagaggatat gcatcagcga gaagtgcatt
    61561 aacgcgtggg tgaagtaacg ccagaactca aatttcagtg ttggatcgaa tggccaggcc
    61621 agccataaca tcacttcctg atcgccgaga atttgcatgg cgataaacac caccacgcag
    61681 gcgatcatca ccacccaggt taccggacct gcgcgttcac gcaaggcagc aaagaaagga
    61741 taacggcgat aatgcagacc actgccggta tggcccgcct gccagctggc cgccagataa
    61801 cgcggatctg ccgggttttc gagaaaacgc gccagctccg cccgcacgcg ctcggcctgg
    61861 gactcatccg ccagccagac atcgctttgg ttatgttgtt gaatcgtaag gataacaccc
    61921 tgcgtcgcca tgtaatcaac aaacgcctgc gccacgcggg ggttagcaaa agaggtaatc
    61981 atcaacatcg ttgctgtcgc ttattccaca caaaagggga cagtataaag cgttacgcgc
    62041 cgtacgccac ctctgcggga aactgacgct gccaggcttc aaaaccgccg tcaatgctat
    62101 agaccacatc gtagccctgt tgcagcagat actgcgccgc gcctttgctg ctattgccgt
    62161 gataacacat caccatcacc ggagtgtcaa agtcgttatc acgcataaac gcgcccagcg
    62221 tgtcgttggt taaatggaaa gcctgcaccg catgccccat tgcgaaactc tgtggatcgc
    62281 gaatatcgac cagcaccgcc tctttttcct gcaacttctg gtgcgcgacg gcaacgttaa
    62341 tacattcgaa ctgatccatg cgtctctctt tctttacaaa caagtgggca aatttaccga
    62401 acagtttacg tcgaagcggc agataaacgc cataatgtta tacatatcac tctaaaatgt
    62461 tttttcaatg ttacctaaag cgcgattctt tgctaatatg ttcgataacg aacatttatg
    62521 agctttaacg aaagtgaatg agggcagcat ggaaaccaaa gatctgattg tgataggggg
    62581 cggcatcaat ggtgctggta tcgcggcaga cgccgctgga cgcggtttat ccgtgctgat
    62641 gctggaggcg caggatctcg cttgcgcgac ctcttccgcc agttcaaaac tcattcacgg
    62701 tggcctgcgc taccttgagc actatgaatt ccgcctggtc agcgaggcgc tggctgaacg
    62761 tgaagtgctg ctgaaaatgg ctccgcatat cgccttcccg atgcgttttc gcctgccaca
    62821 tcgtccgcat ctgcgcccgg cgtggatgat tcgcattggt ctgtttatgt acgatcatct
    62881 gggtaaacgc accagcctgc cgggatcaac tggtttgcgt tttggcgcaa attcagtgtt
    62941 aaaaccggaa attaagcgcg gattcgaata ttctgactgt tgggtagacg acgcccgtct
    63001 ggtactcgcc aacgcccaga tggtggtgcg taaaggcggc gaagtgctta ctcggactcg
    63061 cgccacctct gctcgccgcg aaaacggcct gtggattgtg gaagcggaag atatcgatac
    63121 cggcaaaaaa tatagctggc aagcgcgcgg cctggttaac gccaccggcc cgtgggtgaa
    63181 acagttcttc gacgagggga tgcatctgcc ttcgccttat ggcattcgcc tgatcaaagg
    63241 cagccatatt gtggtgccgc gtgtgcatac ccagaagcag gcctacattc tgcaaaacga
    63301 agataaacgt attgtgttcg tgatcccgtg gatggacgag ttttccatca tcggcaccac
    63361 cgatgtggaa tacaaaggcg atccgaaagc ggtgaagatt gaagagagtg aaatcaatta
    63421 cctgctgaaa gtgtataaca cgcactttaa aaagcagtta agccgtgacg atatcgtctg
    63481 gacctactcc ggtgtgcgtc cgctgtgtga tgatgagtcc gactcgccgc aggctattac
    63541 ccgtgattac acccttgata tccatgatga aaacggcaaa gcaccgctgc tgtcggtatt
    63601 cggcggtaag ttgaccacct accgcaaact ggcggaacat gcgctggaaa aactgacgcc
    63661 gtattatcag ggcattggcc cggcgtggac gaaagagagc gtgctgccgg gtggtgccat
    63721 tgaaggcgac cgcgacgatt atgccgctcg cctgcgccgc cgctatccgt tcctgactga
    63781 atcgctggcg cgtcattacg ctcgcactta cggcagcaac agcgagctgc tgctcggcaa
    63841 tgcgggaacg gtaagcgatc tcggggaaga tttcggtcat gagttctacg aagcggagct
    63901 gaaatacctg gtggatcacg aatgggtccg ccgcgccgac gacgccctgt ggcgtcgcac
    63961 aaaacaaggc atgtggctaa atgcggatca acaatctcgt gtgagtcagt ggctggtgga
    64021 gtatacgcag cagaagttgt cgctggcgtc gtaaggatga tgcccggttg tgaagtgcaa
    64081 ccgggctatt tggattcctt agactattca ttgtaaataa ttgaataacg gcccctgagc
    64141 taaaatcgcc aaacttaagc acgttaccca atcgccccac ttcttcctct ggtttctatt
    64201 ttttctgcac caatggcaat aatatcccaa tgcaaaacca agctgtgcac taatcgggcc
    64261 gacaaaatca gtccaaggct gcggccaaaa aggggaaaaa ttcaggggaa gccagacaaa
    64321 cgtcagaaag aaatttcgga tattttccag caacgctgat ttctctggag aaatataaag
    64381 aatgaaattc gagagaatta accagaaata gaccggcgaa aagaccagcc agcaatgcag
    64441 tctgtttttt aattcaggag gcgttctgcg ggagaattgc caaccgatgc cagccattac
    64501 actgaaaaaa agtgcatatt caaccattaa ctcaagttgt agttcctgaa gagaaatacg
    64561 cacaaccagg agcggaaagg agaatattat ttctgccaga aaagttaacg tgaatataag
    64621 tacggctaag acaataaccg tcggccatac caataatggc acgataacaa aaccgagaat
    64681 tacaggggga atataaagcc ggaatttaat catttttatt ttcctggtga ttcggatgat
    64741 gcgtcataca tcgcgcataa acactcatca attatccagt cgtctaccca gacctgatgc
    64801 tgtgtgccct gttctggctg ctttggtggg ttctcaatcg cctgccgcgc gcgttctttc
    64861 aagatggatt gattgttgaa ggcccaggca ttgatttggg tggtaatgac gttatcgata
    64921 tcgtcctggt tatctctgat ataatcctca caatatctgt tcaataaaca ggcaagagaa
    64981 acggtcgacc aaaagccggg aactgaatta atggcatcca gcaatggcgc aagagcgata
    65041 tcggcacgga aaaacatttc cagcatatac tgtaattcga cagcttcatc gccggatacc
    65101 caatcagtca tatactggca ggcaaatcgt tgcaaaattt tccattcatc ctcacgccaa
    65161 ttttctttac tggcaaggtt tatacgccgt agagaaaata cttcgtgaat attgtcaaac
    65221 tgcccctgtg caacaaattc cagtaaacgc ggcagaaaat agcggacctc atcgctattt
    65281 tgcggatcag gccctgggct actattccat gcattgatta aggaatacga gatcgctcgt
    65341 aacgatgtac tccgtaatac cttttgttcc tgctgactca ggcaatattc acaacaaacc
    65401 acaaatttct gcggcaccgg ataacgacga aaaatatgat aaatctgctc aattaatgcc
    65461 tgcatggcgg ggctgggttg cataattacc ccctcgcccg ctgatcgcgt gtaaacggtt
    65521 gataaacaaa acgagcattc gccacgttca ggcgaataat ggcactacga gagagggaaa
    65581 agctggggcg aatgcaacta ttgcagtctc gacagctttc ttcagcgcaa ttttccactg
    65641 tgtaataacg agcgtagagt ttcagttccg cttgcttatc acgaaatccg cgtaaactgt
    65701 tatcgttatg aaatttccag gcatctttaa agacctgaaa aatgtagata tcctcaatcc
    65761 aggactcaca ttttttaagc gggcgctttg ctatgatcac ccgctcatcc gggtttagca
    65821 tcgccgtaaa ttgaacggtg aggtcatttg tcgtgttcag atcacattga acatctggta
    65881 catcactgat ataggggtca aatccaaagt cagcaccaca ggcacaaaga cggctaatgg
    65941 tggcggtatc gagggaaaat cccggccata cattgctgga cgttaagcca atatttaatg
    66001 tgacattata tcgaagcaat ccctcaagac cgtcagcacc gacgaacttt tccccttcat
    66061 ccagggcctg gttaatgagt tgctgaacaa tgccttcatc ccagtatgcc gatttgcagt
    66121 tggcaatcgt aatatccacg cctgtgaatg gatcagtacc gacaaggctt gcaaataatg
    66181 tattaactaa ttccattttt attatccgcc ttaatgcaac gttttataca tcggcacata
    66241 acctacatct tccaccaggc tctgcccctg cggcgttaaa aaccactcca ccaatttttg
    66301 tgtttctgac gtcatatttt cccgcgttac cataaaggca tcgacgatat agggatattt
    66361 gccgttgcga atgttttcgg cagtcggtgc aataccgtta atcgctagca gttttatatt
    66421 tttatcggcg ttcatttgtg ttgcgtaata acggaaggtg taacctattg cgttgtttgt
    66481 attacggtat tcggcaacga ctttaatcat tccctccatc atacttgcca cttctgtttc
    66541 ctgcggcgag atcatgcgga catttttcat gacctgggat tgcatcaccg tctggctacc
    66601 agagtcttcc gggcgttgcc aggtctggat ctcctgatcg ttaccgccaa ccgtgcgcca
    66661 gttggtaatt gcaccactga agatgtcacg cacttgttgt tcggtcaggg aattaaccgg
    66721 gttatctaca ttgacgatga aaacaaacgc ttcacgggca aaaggcgtgt aaatcaaagt
    66781 gacgcccgat tcctccgcgc gttttttctg cccaccggaa ggttgagcca caaagataat
    66841 atcggcatta cctttaacaa tcttgttata tgcttcggga gtacgagagt tcgccagata
    66901 ttcccattcg tgaaaatctt ccggcaaaac gcttagtgca taaaaggcag aggcataaat
    66961 ggggtacgcc gccgttgcgc catcaagtcg cggccagttt tgcgtgaact gaatttgcgg
    67021 tttaccacgc agtggtgtca gttgattatt gagtttatcg ggtcgccagg cccaggtatc
    67081 aatattttca cgcacaccga cgccgggatt aagctgttta tataaataag cctggtaacc
    67141 ggcgacaaaa ccaagcatga gaaggataaa caagagagaa ttacggcaac gtaacccgga
    67201 cgtgcccgtt aaaggatatc tggtcggcca gccgtacccc agcgaaaaac tgatatgcgc
    67261 gccaacaggt ataatgacta accaccagta ctcaccaccg actaatgagg ctaacaaaac
    67321 gcccagccag gggagcataa gatcagccaa ccaacccgct cccatctggc gaaattgata
    67381 atctgccaga tgcagcgcga ttatccagac ccccagggtt aataatgcgg gtactaaaaa
    67441 aagacattgt cgccagacgg ctatttctct tttttctggt gcactatcgt ccgcccacac
    67501 tcgccccgag aaaaatagca ttaatgcgcc cagcgccagc ataaaaatgt tgagcagaag
    67561 gtaaattgtt ggcgtgataa agatattaca aacttcaata ataccggcaa gtccgacacc
    67621 cagcatggca ataaccgccg ccaaaaactg cgccagaatg gggatgccga aaaaagtcat
    67681 taccagcgag gtcaaaatcc attttctgtt ttgcattatt ctttccattc tttctgaatg
    67741 gcgaaattat actccccgag tccccttgcc ccttctggac acttttccga aatgatggcg
    67801 aaaaaaacgg gaccctttgg ccccgttcta tttattagtg aaattacaat ctcaccggat
    67861 caatatgcca gatatgatcg gcgtactctt tgatagtacg gtcagaagag aagtagccca
    67921 tattggcaat gttcagcatc gcttttgcgg tccactcttc ctgaagctcg tagagttcgt
    67981 cgactttatc ctgacaatcg acatagctgc gataatccgc cagaacctga tagtgatcgc
    68041 cgaagttgat cagcgaatca accagatcgc gatagcgacc cggatcttcc ggactgaata
    68101 caccgctgcc gatttgcgtc agcacctgat gcagctcctc atctttctcg tagtattcac
    68161 gcggtttgta gccctgacga cgcagttctt ccacttcttc cgcagtgtta ccaaagataa
    68221 agatattgtc agcaccgaca tgatccagca tctcgacatt cgcaccgtcc aacgtaccga
    68281 tagtaagcgc accgttaagc gcaaacttca tgttactggt gccggaagct tccgtccccg
    68341 ccagcgaaat ctgttcagac agatcggccg ccggaatgat caactgcgcc aggctaacgc
    68401 tgtagttcgg gatgaacacg actttcagct tgtcgccaat ctgcggatcg ttgttgatca
    68461 ctttcgctac gtcattgatc aaatgaataa tgtgcttcgc catgtaatag gccgaagccg
    68521 ccttaccgcc aaaaatattc acgcgcggta cccacttcgc atccgggtcg gccttgatgc
    68581 ggttatagcg ggtaatcaca tgcaacacat tcatcaattg acgtttgtat tcgtgaatac
    68641 gtttgatttg tacatcgaac aacgcctttg gattcaccac cacattcagc tgctgggcga
    68701 tatactctgc cagacgcttt ttgttctcca gcttcgcctg atgcacagcg tgattaacca
    68761 ttgggaaatc acagtgttgt tgcagctcat taagcaggct aaggtcggtg cgccagttac
    68821 ggcccaggtg ttcgtccagc acggctgaaa gcgatgggtt cgctaccgct agccagcgac
    68881 gcggcgtcac accgttggtg acgttggtga aacgacccgg gaagattttc gcaaagtcgg
    68941 caaacaacga ttgcaccatc agattagagt gcagttccga tacaccgtta actttgtggc
    69001 tcacaacaac cgccagccag gccatacgca cacgacgacc gttggattca tcaatgatcg
    69061 acgcccgtcc cagcagatcg gtatcgttcg gatactgttc ctgcaaggtt ttcaggaaat
    69121 agtcgttgat ttcaaagatg atctgcaggt gacgcggcag aattttaccc agcatatcaa
    69181 ccggccaggt ttccagcgcc tcgctcatca gcgtgtggtt agtgtaggag aagacctgac
    69241 aacacacctc aaacgcgtcg tcccagctaa attggtgctc atcgatcagc agacgcatca
    69301 tctcaggaat cgacagtacc ggatgggtat cattgagatg aatcgcgatt ttatccgcca
    69361 ggttatcgta ggttttatgc aactgataat ggcggcttaa aatgtcctga atggtcgagg
    69421 aaaccaggaa gtattcctga cgcaggcgca gctcacgccc ggagtaggtg gagtcatccg
    69481 gatacagtac gcgagatacg ttctcggagt ggtttttatc ttccactgcc gcgaagtagt
    69541 caccctggtt gaatttaccg aggttaattt cgctactggc ttgcgcactc cacaaacgca
    69601 acgtgttggt cgcgtcggtg tcgtaaccag ggattatctg atcgtaagcg actcccagaa
    69661 tctcttcggt ttcaatccaa cgcgtttttt taccttcctg ctgaatgcga ccaccaaaac
    69721 ggactttata gcgcgtgttg tggcgtttga attcccacgg gttaccgtat tccagccagt
    69781 agtctggcga ctctttctgg ctaccgttaa cgatgttctg cttgaacata ccgtagtcat
    69841 agcggatgcc gtaaccgcgc cccggcaacc ctaacgtcgc cagagaatca aggaagcaag
    69901 ccgccagacg tcccaggcca ccgttaccga ggcctgggtc attttcttca tcaatcagct
    69961 cttcgagatt taaccccatc gcttccagtg cgccctgtac atcttcgtaa attcctagcg
    70021 acaacatggc gttggagagc gtacggccaa tcaaaaactc catcgacagg tagtaaacct
    70081 gacgagtttc ttgcgacaac tgggcacggt ttgaacgtaa ccagcgctcc acgagacgat
    70141 cgcgcacagc aaataacgtt gcgttcagcc attcatgttt attggcgacg accgggtcct
    70201 ttccaatcgt aaacatcagc ttgtaagcga tagagtgctt aagagcttct acgctaagcg
    70261 tgggcgatga atatgtaaac ggagcattca tataggcgtt tcctgaaaac tatttcaagc
    70321 gatagtaaag ctcacggtac gacttcgccg cgacctgcca gctaaaatcc attgccatag
    70381 cctgacgttg cacaaaccgc cacagtgaag gacgggacca cagtacaaaa gcacgtcgaa
    70441 tagcccgtaa cagcgaccag gcattactat cttcaaagac aaacccactg gcgacgccat
    70501 ctgcaaggtt ctcaagagaa cagtcagaaa ccgtatcagc aagcccaccg gtgcgccgca
    70561 ctaacggcag cgtaccgtac ttcaatccat aaagttgcgt taagccgcac ggttcaaaac
    70621 ggctgggcac cagaatgaca tccgcgccgc ccataatgcg atgcgaaaat gcttcgtgat
    70681 agccaatctg aacgcccacc tggccggggt attccgctgc cgccgcaagg aaaccttcct
    70741 gcagcaccgg atcgcccgcg ccgagtagcg ccagctgccc gccctgctcc agaaggcccg
    70801 gtaaggcttc cagcaccagg tcgagacctt tctggctggt cagacggctc accactgcaa
    70861 aaagcggcac tttatcatca accttaagcc ccattgcgat ttgtaactgg cgcttatttt
    70921 ccgctttatc ttccaacgta tcgcgggtgt agcgcgaggc caacagtaag tccgtctctg
    70981 gactccagat tttctcgtcc acgccgttca gtacgccgga aagacgccct tcacggtgac
    71041 gctgttgcaa cagaccttcc ataccgtagg caaactgcgg ttcggtgatc tcgcgagcgt
    71101 aggttggact gaccgccgta atgtgatcgg catagtacag accggccttc aggaaagaga
    71161 tttgtccgtt gaattccagc ccatgaatat taaagaatga ccatggcaat tggatgtcat
    71221 tcatatgatg tgcataaaac atgccttgat aggccaggtt gtgcacagta aacaccgact
    71281 tcgccggacg cccgcgcgcc gccagatacg caggcgcaag gcctgcatgc cagtcgtgcg
    71341 catgcaccac atcaggacgc cagaatgggt caagcccgct ggccatttct gccccaaccc
    71401 accccagcag cgcaaaacgc aatacgttgt cggtataggc aaataagttg gtatcgtgat
    71461 acgggcttcc cggacgatca tagagatgcg gcgcgtcaat caggtatatg ccaaccccgt
    71521 tgtaatgacc gaacaacagc gtgatatgtc cggcgaaggt atcacgacgg gatactacct
    71581 gcgcatcggt aacgccacgg cgaatatcgg gaaatgcagg caacagtacg cgagcgtcaa
    71641 cgccgtctgc gatttgtgct gcgggtaatg ccccaataac atcagccaga ccgccggttt
    71701 taagcagcgg gaacatctct gaacatacat gtaaaacctg cattatcgct cctgtttatg
    71761 ccctaacttc cgtagcattt cgcgcgttac cagcacgatg ccttcttctg aacgatagaa
    71821 acgacgtgca tcttcctctg cgttttcacc aatcaccatg ccttccggaa taacacaagc
    71881 acgatcgatg acgcagcggc gcagacggca cgagcgacct acccatactt ccggtaacaa
    71941 tacggcggaa tcaatgttgc agaatgaatt cacgcgaacg cgcgagaaca gaacggactg
    72001 caccaccacc gaaccggaga tcacacagcc gccggatacc agtgagttaa gggtcatccc
    72061 gtggctaccg gagcgatcct gcacgaattt cgctggcggt aatgattcat tgtaggtgcg
    72121 aattggccaa ttgcgatcgt acatatccag ttccggcacc acagaggcca gatcgaggtt
    72181 cgctttccag taagcttcca gcgtacccac atcgcgccag tacggctcgg catccgggtc
    72241 ggattgtacg caagagagcg ggaacgggtg cgcataggcc agaccggctt cggtgatctt
    72301 gggaatcaaa tctttgccaa agtcgtggct ggagttctca tcacgatcgt cttcttccag
    72361 cagttcatac agatagtcgg cgtcaaagac gtagataccc atactcgcca gagatttgct
    72421 cggatcgttc ggcattgacg gtgggttagc aggtttttca acgaattcga taattttatc
    72481 gttctcatca accgccataa cgccaaatgc ggaggcttct tcaatcggta ctggcataca
    72541 agcaacggtg caacgtgcgc ctttttcgac gtgatcgata agcatacgcg agtagtcttg
    72601 cttgtagata tggtcgcccg ccaggatcac cacgtattcc gctttataac ggcggataat
    72661 gtcgaggttt tgggtgaccg catctgcggt gccgcgatac cagttttccc ctttcattct
    72721 ctgctgtgct ggcagcagat cgacaaactc gttcatttct tcattgaaga atgaccagcc
    72781 gcgctgaatg tgctgcacca gagtgtggga ctggtactgg gtgatcacgc ccatacgacg
    72841 gatcccggag ttgatgcagt tagacagcgc aaagtcgata atgcggaact taccgccgaa
    72901 gtgtacggcc ggttttgctc gcttattggt taaatccttc aggcgggtac cacgtcctcc
    72961 cgccagtatc agggcaacag atttcaatgg cagctggcgc gccaacatta agtgatcgtt
    73021 cttctctaaa ctaaccatga ctaactcctt ttttatcatc tctggaacac acacaatccg
    73081 tgtgcaggtc cctgccagac agccgtaatc actgggttat cctctccagc gaatggggga
    73141 atggcgtgcc actccccagc aggtaaaaca atctctgtta cctcaagcgt ggcgttaatt
    73201 gcgatcaaaa agcgatccga gagcagaatt tgcagctgtt tcggcccgtt ttgccactca
    73261 tccgtgctta aaggttgagc atatcgattt agccaacgga cattgccgtc gccttcttcc
    73321 caccagcgat tctccaccaa agcgggaatg cgcttgcgca gatggattaa cgcggcggta
    73381 aatgcggtta aaccactgct tgcctgcgac cagtccaacc aggttaattg gttatcctga
    73441 cagtaggcat tgttattgcc atgctggctg tgaccatgtt cgtcaccggc cagtaacatc
    73501 ggcgtaccct gggagagcaa caacgttgtt aacagggcgt gaatgctgtc gcgccgccgt
    73561 tcaaccaggt caagagaacc gcctaaccct tctttaccat gattgttact gtaattgttg
    73621 ttggtcccgt cgcgattttc ttctccgttt gcttcattgt gtttatggtt gaagcaaacg
    73681 cagtcgcgaa gcgtaaaacc gtcatgcgcg gtgacgagat taatcgcggc actcggcaga
    73741 cgaccattac gtttaaaaac atcgctggag gcagcaaaac gcccggcaaa cgcccccaga
    73801 ggcaaatcat aatgtagcca gaaacaacgg gcagcatcgc ggaaatgatc gttccactcg
    73861 gcaaacagcg gcgggaaatt tcccacctga taaccaccag gagcgatatc ccacggttca
    73921 gcaattaact tcacctgcga gagcaccggg cagttctgga tagcggtaaa caacggcgca
    73981 tcctgacgga actctggcgt acggcccatg actgccgcca gatcaaagcg gaaaccatcg
    74041 acgtggcagg tttctaccca ataacgcagg caggcgctgg cataatccac caccgccgga
    74101 tgactcaaat tgagcgtgtt gccgcaaccg gtccagttgt gataatcgcc gtcttctctt
    74161 atccaataat agctacggtt atcgatccca cgcagcgaga ataacgggcc gtcgaggtcc
    74221 agttccgcac tatggttgag cacgatatca agaatgactt cgatacccgc tttatgcagt
    74281 gctttgattg catcgcgaaa ctcatccagc gccgtttctg gcgagcaggc atacgccgga
    74341 tgcagcgcaa acatcgccac cgggttgtaa ccccagtagt tacttagccc catgcgttgc
    74401 agacgtggtt cactggcaaa ctgcgccact ggcagcagtt ccagcgcggt aatgcccaat
    74461 tgtttcaaat agttgatcat caccggatgc ccgagggctt tataagtgcc acggatctcg
    74521 accgggatct ccgggtgcaa gtacgttaat cctttgacat gggcttcata aatgatggtg
    74581 ctgccccacg gcgtgcgcgg cggggcatca tcttcccagt catagtgatc aaccaccact
    74641 acgcatttcg gcgcaatggc ggcgttgtcg cgatagtcag gttcattatg accggcgtgc
    74701 agcagcgggt tatctttaaa ctccccctca atttgccgcg cgcaaggatc aatcaacaac
    74761 ttcgccgggt taaagcgatg cccctcggcg ggttgccagg ggccatgaac gcgataacca
    74821 taacgcaaac ccgggcgcgc atccggcaga taaccgtgcc aaatgtcgcc actgtgccct
    74881 ggcaagtcat agcgatgttc ctggccattg gcgtcaaaga cacacagttc tacccgctcg
    74941 gcatgagcgg agaaaagtgt gaagttgacg ccctgaccgt cgtaatgcgc gccgagggga
    75001 gcgggtttgc caatggcgag ttgtgtcatt ctgcctcccg aaccagccag atagtggcca
    75061 gcggtggtag cgtcaggctt agtgaatgct gacgaccgtg gctggcaatc tcatcgctgt
    75121 gtaccgtgcc gccattgcct gcattactgc cgtgatagtg catggaatcg gtattgagga
    75181 tttcacgcca tttgcccggc tggtttatgc cgaagcgata atcatgacgc ggcaccggcg
    75241 taaagttact ggcaacgatg atttcgttac cctctttatc gcgacgcaca aagatcagca
    75301 ccgagcgttc tttgtcatcc accaccagcc attcaaagcc gtacgggtca aaatccagtt
    75361 catgcattgc tttatggtgg cggtaggtga ggttcagatc gcgcaccaga cgctggacac
    75421 cgtggtgcca gttatcgccg ccttccaaca gatgccagtc gaggctggcg tcatggttcc
    75481 actcgcggcc ctgggcaaat tcgttaccca tgaacagtag tttcttgccc gggaatgccc
    75541 acatccagcc atagtaggcg cgcaggttcg cgaatttctg ccatgcgtcg cccggcatgc
    75601 ggtcgagaat cgatttttta ccgtggacca cttcatcatg cgacaacggc aggacgaagt
    75661 tttcagtgta gttgtagaga atcccgaagg tcagtttatc gtgatgatac tgacgataaa
    75721 ccgggtcgag cttcatgtag tccagggtgt catgcatcca gccgaggttc cacttgtacc
    75781 agaagcccag accgcccata tcctgcggac gagaaacgcc agggaaatcg gtagactcct
    75841 cagccattgt caccgcaccg gaaacctgct caccaagaat acggttggta ttacgcaaga
    75901 attcaatcgc ttcaagattc tcgcgcccgc caaattcgtt cgggatccac tccccctctt
    75961 tacggctgta gtcgcgataa atcattgacg ccaccgcatc gacgcgcagc gcatcaatac
    76021 caaaacgttc aatccagtaa agcgcgttac cgacgaggaa gttactgact tcacggcgac
    76081 catagttgta gatcagcgtg ttccagtcct gatgatagcc ttcacgcgga tcgctgtgtt
    76141 catacaagtt cgtgccatca aattcggcaa gcgcaaagtc atcagtcggg aagtggcctg
    76201 gcacccagtc gagaatcacg ttcagaccag ctgcgtgtgc ggcatcaatg aaataacgga
    76261 agtcgtcgcg agtaccaaaa cggcgggttg gtgcatacag gccggttggc tgataacccc
    76321 aactgccatc gaagggatgc tcgttaatgg gcagtagttc gaggtgggta aagcccatcc
    76381 atttagcata aggcaccagt tgatcggcca gctcgcggta gctcaaccag aaattgttgt
    76441 cggtgtggcg acgccaggaa cccaggtgaa cttcataaat agagattggc gcatcaaact
    76501 gattcgcttt tttgcgctct tcagtctgta caaccttttc cggcagcccg caaataagag
    76561 acgcggtttc cgggcgcatt tgcgcttcga aggcataagg gtcggacttc agacgcaagt
    76621 tgccattggc atcaatcatc tcgtatttat agagctgacc gttatgcgcc ccagggataa
    76681 acagttccca gatgccgctc tctttacgca ggcgcatcgg gtgacggcga ccgtcccagt
    76741 agttgaattg cccaaccacc gagacccgac gggcgtttgg agcccagaca gagaaacgcg
    76801 tacctgtgac gccatccata gtatctgcat gcgcgcctaa ggtttcatac gggcgcaggt
    76861 gagtaccttc agataatagc caggcatcca tttcctggat tagcggacca aaacggtaag
    76921 gatcatcaat caggttttgc tgaccatgcc agacaacagc caactgatag cggaaaaaat
    76981 tcttacgtcg cggaatgacg ccgctaaaga atccccgtga gtcgagacac tccagttttg
    77041 cgagtttgcg cccggttttc ggttcaatca cccacacatc ggtagcgtcg ggtaaaaggg
    77101 cacggacttc cagtcccgcg gtggttttat gcattcccag tacggaaaaa ggatccgcaa
    77161 aatggcctgc aattagcgcg ttaatcacgt ctctatcgat acgatcggac atgcttgtct
    77221 tcctgtttta ttgtgtcacc ccatccaatt ggatttttga cttctggttg tgacattttt
    77281 ttgacctgaa cggcgcagca ctctgtgcat cctctctgcg tcgtcctcac ttcaggtaag
    77341 gctgtgaata ctcatgtatt cagccaccct taaagaatag ccaatgctct atttaactcc
    77401 cggtaaatca tgaaacatct gcgcttactc ctgtattacg cactaacagg ggcggcatcg
    77461 cgccccagat ttaatgaata aagattacgc cagttgacga agcatccgac gcagcggctc
    77521 cgcggccccc cacagcagct ggtcgcccac ggtaaaggct gacaggaact ctggtcccat
    77581 attcagctta cgcaggcggc ctaccggcgt ggtcagcgtg ccggtaacgg cagctggggt
    77641 tagctcacgc atagtgattt cccgatcgtt cggaacgact ttcgcccacg gattgtgcgc
    77701 agccagcagt tcttccacgg tcggaataga cacatctttt ttcaatttaa tagtgaatgc
    77761 ctggctgtgg cagcgcaatg ccccgacacg cacacataaa ccatctaccg gaattacgga
    77821 agatgtgttg aggatcttgt tggtttccgc ctgccctttc cactcttcgc ggctctgacc
    77881 gttatcgagc tgtttgtcga tccacggaat caggctaccc gccagcggca cgccaaagtt
    77941 atccaccggc agctcaccgc tacgggttaa ggttgtgact ttgcgttcga tatcgagaat
    78001 agcagaggac ggggtcgcga gttcatctgc cacatggcca tacagatggc ccatctgggt
    78061 taataactca cgcatatgtc gcgcaccacc gccggaagcg gcctgatagg ttgcaacgga
    78121 cacccaatca acaagatcat tggcgaataa accacccaac gacatcaaca tcaggcttac
    78181 ggtacagtta ccgccaacaa aagtcctgat gccattattt aatccgtcgg taatgacgtc
    78241 ctgattgacg gggtcaagaa tgatgatggc gtcatctttc atgcgcagag acgatgctgc
    78301 gtcaatccag taaccttgcc atccgctttc acgaagcttt ggatagattt cgttggtata
    78361 atcgccgccc tgacaggtca caatgatatc gagggccttt agcgcctcca gatcaaaggc
    78421 atcctgaagt gtgccagtgg ttccgccaaa agacggcgca gcctggccaa gctgagaagt
    78481 agaaaagaag acagggcgaa tggcgtcgaa gtcgcgctct tcaaccatgc gttgcatgag
    78541 aacggagccg accataccgc gccagccgat aaaaccaaca tttttcataa gcgttttttt
    78601 cctgcaaaga tgtgtgctgt ataaatgtgc cggtctcctc ttggcacatc tttcaccata
    78661 caaaaagcag ccaaagtcgc aagtgaaatt aatcaatgat agcgaagcca tcagtaatgc
    78721 gacttatcct gctttgttag cacgcagaaa gtccgcggca attatcaggg aatttgagtt
    78781 atgaatgaaa tcatttctgc agcagtttta ttgatcctga ttatggatcc gctcggaaac
    78841 ctacctattt tcatgtccgt actgaaacat actgaaccga aaagacggcg ggcaatcatg
    78901 gtgcgagagt tgcttattgc tctcctggtg atgctggtgt tcctgtttgc gggtgagaaa
    78961 attctggcat ttctcagcct gcgagcagaa accgtctcca tttctggcgg catcattctg
    79021 tttctaattg ccattaagat gattttcccc agcgcttcag gaaatagcag cgggcttccg
    79081 gcaggtgaag agccatttat cgtgccgttg gcaattccgt tagtcgccgg gccgactatt
    79141 ctcgccacgc tgatgttgtt gtctcatcag tacccgaatc agatggggca tctggtgatt
    79201 gctctgctgc tggcctgggg cggcaccttt gtcatcctgc tacagtcttc gctattttta
    79261 cgtctgctgg gcgagaaagg ggtgaacgca cttgaacgcc tgatgggatt gattctggtg
    79321 atgatggcaa cccagatgtt cctcgacggc attcgaatgt ggatgaaggg gtaacgagga
    79381 gagtttttcg aaaagaaccc ggtagcgtca caactaccgg gcgaacaaac ttaactcaac
    79441 agctggaacg caatcatccc aacgatggca ccgacagtgc cgaggatggt ttccatcatg
    79501 gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa caaccagaaa
    79561 ccggcgtcgt taacgtggct gacaacaatc gaaccaccag cgatacaaat cgacagcgcc
    79621 gccatttgcg caccggagta gttcagttgt tcaataaccg gcatcaccag tcctaccgcc
    79681 gttaaacagg ctacggtggc agaaccctga atgatgcgca ctgcagctgc cagcacgaag
    79741 caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc cggacctacg
    79801 ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag cagaatgatc
    79861 cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg catgccctga
    79921 cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt aaacggatga
    79981 ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg cgcggcaatg
    80041 gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa tccgaaagat
    80101 ggcattttgc cttcgccgag atgcggttcg ctgatgtcgt caggaatatg caactccacg
    80161 tagcggctga tgaaattacc ccacagcggc ccggcaataa tcattcccgg aattgccgca
    80221 cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc cagcagcatt
    80281 ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa taatgggatt
    80341 accagcttca ccaggttcgt accggtgtgg cgcgccattg agaaagcaac gctaatcagc
    80401 agaactatcg ccacttcaaa gaacagcggt agcgcacaga ccaaccccgc aaggccgatg
    80461 gcaaaatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat ctgatcgact
    80521 gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac caccgccagg
    80581 aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc gagcggcata
    80641 ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc gtgcatacgc
    80701 gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa aacaagcgtt
    80761 aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc cacaacacct
    80821 tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc cggctcctgc
    80881 agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt gcgcgctttc
    80941 aagcgacttt caatcacatc aaaatcgcct ttcaggtaga tgaaagagag attcggatta
    81001 ccttcacgca gcaagtcgcg atagtgcttt ttcagtgcag aacagacgat cagcgacact
    81061 ttattggtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcagcca cggtttgcga
    81121 tcgtcatcat tcagcggttc gccagacgcc attttttcga tattgcgccg tggatggagg
    81181 aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc gaccgcagat
    81241 ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt agtcgtgctc
    81301 aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt atcggtaaca
    81361 ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt ataagtgtga
    81421 gctacttcaa atttgtgggc ttaaatagat ccgcccggtg acaaggtgaa acctaaatct
    81481 aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc agcgccaata
    81541 ctgcccatcc gctcacgcgg cgtcagcacg ctcgccagtc gtggctccat cacctgacca
    81601 atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaacttttaa ccccagacgc
    81661 tgacattcaa acgccgcgcc gaccgccaga tcatcattag tacagaacac gccatccagc
    81721 tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta agaagaagat
    81781 tgctcaacca tcacgctata cggcaccagg cctgcatcca gcatcgcctg ttcgtatccc
    81841 ttctgtttga tgatagtacg ttcgtcgaga cgtgcgccga gataggcaat gtggcgatgc
    81901 ccgcgcgcaa taatggcggt agtcatctgg cgtgccgctt caaagttatc aaaaccgacg
    81961 gcgatatcga ggcacggcga ctggctgtcc atcaactcca ccaccgggat ccccgccact
    82021 tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag gccgtcgata
    82081 ttccaggaca gcatggattc gagacgttct tgctcgattt ccggtttata accgtagtgc
    82141 gccagcatcg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg taatacttcc
    82201 gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct ggtagcgtta
    82261 gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc ggcggcaatc
    82321 ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct gaccgtcatt
    82381 ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt tttcatcgtc
    82441 ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag aaccttcccg
    82501 gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc atcgcattgt
    82561 gctaacgacg cggcaaaacc ccggaagcgt gcatcagcac gcgaccggag cgagcgagga
    82621 agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg gcagatcgaa
    82681 cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat cccagattgc
    82741 cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt tcaccacctg
    82801 gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt tcagcaacgc
    82861 ccaacgatac agttccatat cctgatgcac tttcagcgaa ccatctcgcg catccggcga
    82921 gagcaccagc tgtttgccct gcacggcatc gaagcgacgc tgttcataac gcggcgtaat
    82981 gccattttct tcaggcatga tccagatctg atacagatgc agacgctcgg tgctgtttgg
    83041 gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact cacccgccgg
    83101 aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca gcacgtaggt
    83161 caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt caatcacgtc
    83221 gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat agttggcaaa
    83281 agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt catttgcttt
    83341 gcggaagtag atcatttgta ttcgccccct gaatgatttc gatgggctta gtgtggaccc
    83401 gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa attgaactat
    83461 tcgaggggcc atcaaactta cttagcgaga gttactgtgg agggagaggc ttgctcaaat
    83521 ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta atttggcgca
    83581 ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc catctccggc
    83641 ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac accgaccgaa
    83701 tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct ggtttcctgc
    83761 tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa tttcggataa
    83821 tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata aaacagttgc
    83881 gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcggatgtc ataagcgacg
    83941 tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc aaggccataa
    84001 aacgcgccat cctgcggcag cccaggtttg gtttctgcca ccgggcggta atagtcaaaa
    84061 tggctttcca cttcaacaat ctctcccaac ttgccacttt caatcgcttt tttcgctgtc
    84121 aggaagcagg agtcaaagcg acgattctga tacggcgtga cggtcagccc tttgcttttc
    84181 gccaacgcaa acagctcctt cgcctgggca agcgtcgggg tgaacggttt ttcgaccagc
    84241 acatttttcc cggcttccag cgcgcgtttc gcgtactcga aatggctgtc cgcgtgggtg
    84301 cagacaacaa ccagcttaac atcgggatcg tttagtactt cgtcgagatc gctggtcaaa
    84361 tggatatggg aataaatggg agcctgttct tccggcttcg catggcgacg aaaaatatgc
    84421 gcgacatgcc agctatcctt gcggttaagt acatacggca gatggtaacg ggtggtgctt
    84481 ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt aaggtggtta
    84541 tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac gaacacaagc
    84601 actcccgtgg ataaattgag aaagaaagat caaaaaaaaa gccagcaccc ggctggctaa
    84661 gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga gggtcttcct
    84721 gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt tgcaaaaaaa
    84781 tgcatttgac tcgtatttga aagtcaatga tgttgaaagg gacatttacc ccaaagagga
    84841 caaaggaatg agtgagatag taatacgcca cgcagaaaca cgggattacg aggccatcag
    84901 gcagattcac gcccagccgg aggtgtatta caacacgcta caggtgcctc atccttccga
    84961 tcatatgtgg caggagcgac tcgctgctca tcccggcatc aaacagttag ttgcctgtat
    85021 tgatggaacc gtcgtgggtc acctcaccat tgacgtgcaa cagcgcccac gccgcagtca
    85081 tgttgccgat tttggtatct gtgtcgactc tcgttggaag aaccgcggcg tcgccagcgc
    85141 cctgatgcga gagatgattg aaatgtgcga caactggtta cgggtagatc gcattgaact
    85201 aaccgtgttt gtcgataacg ccccggcaat taaggtctat aaaaaatacg gctttgaaat
    85261 tgaagggact ggtaagaagt acgcattgcg taatggtgaa tatgtcgatg cgtattatat
    85321 ggcgcgggtg aagtgaaaac acttaactta ttgtaaatac gaatatttga tgaaggcttt
    85381 gttggatatg ttgcgcttga aaaaaagcac gacttaacta ccccgtcgaa aaattcataa
    85441 taattatgat gatatatcag gaagcattcg cgaaagcgaa ccattacctt gatgatgcag
    85501 atctccctgt cgtcatcact ctgcatggac ggtttagcca gggctggtat ttctgtttcg
    85561 aatcacaaga atttcttgaa tctggtgatg aggccgcgcg tttagccggt aacgcacctt
    85621 ttattattga taaagacagt ggtgaaattc attctctggg aacggcaaag ccgctggaag
    85681 aatatctaca tgattatgaa ataaaaaaag ctaccttcgg cttgccctga caatacggcc
    85741 ctcttcccac gaagagggcc gctaacctta ataccccgcc gttaaatcat ccaccgagcg
    85801 cgggtcggat gcaccgtaca actcaccgtc cggcccaacc ataatgcttt gtgtactacc
    85861 catcgcctct ttcagcgcca ctttctgacc ttttgcttcc agcagcttga gcgtatccgg
    85921 gctaaaccct ttttcgacac gcagctcgtc cggcaaccac tgatggtgga aacgcggcgc
    85981 attggtcgct tcggcgacgt tcatgccata atcgatgcta ttcaccacca tttgcagcac
    86041 tgtagtgatg atccggctcc cgccagggct accggtgacc agccaggttt taccgtcttt
    86101 caccacaatc gttggtgaca ttgacgacag cggacgttta ttcggcccga cggcgttggc
    86161 atcaccgccc accagcccgt aaacgttcgg cacgcccggt ttagcggaga aatcatccat
    86221 ctgattatta agcagaatac cgctctcgcc cgcgacaata cccgtaccga aggtggtgtt
    86281 cagcgtatag gtcaccgcca ccgcgttacc gtctttatcc accactgagt aatgggtagt
    86341 ttgattactc tcataaggcg caagcttgcc agggcgaatc tcactggacg gcttcgcttt
    86401 attgatatcg atttgatcgg caatagattt ggcataggct ttattggtca acgcctgcca
    86461 cgggactttc acaaaatccg ggtcgccaag atattccgag cggtcagcgt aggcatattt
    86521 ctccgcttct gccatgattt gcatcgcatc ggcgctgcca aagccgtatt tcttcatatc
    86581 gaagttttcc aaaatattga ggatttgtac gatatggatc ccgccggaag atggcggtgg
    86641 catggagtaa acctgatacc cgcgataatc gccgcttatc ggtgtgcgtt cgaccgcttt
    86701 atacgccgct aaatcttctt tggtgatcaa gccaccgttt ttctgcatct cctgggcaat
    86761 ctgttccgct atcgtgcctt tgtagaattc gtccgggccg ttttcggcaa tcatctccag
    86821 gctctttgcc aggttcgcct gcaccagctt gtcgcccttt ttcagcggtt cgccctcttt
    86881 ccagaagata gctttgctgt tttcgtgatt cggcaacact tcgctaccgt aggttttgag
    86941 atcgtcagcc agcgcgtcgt taacgataaa accatcgcgt gcaagtttaa aagcgggctg
    87001 cacgacttta ttcagcggca tggtgccgta tttatccaac gccagcgaga aacctgctac
    87061 cgtaccgggc gtgccagagg ccagatgcga agtgagtgat tttttgctgt ccgggttgcc
    87121 ttgatcatca aggaacatat cgcgggtcgc tttagcgggt gccatctcac ggaaatcaat
    87181 agcagtggtg ttgccatctt tagtgcggat cagcataaaa ccaccgccgc ccagattccc
    87241 cgcctgcgga tgcgttaccg ccagcgcgta gcccaccgcc acggcagcat caacggcatt
    87301 cccgccctcc ttgaggatat ccacccccac ctgagtggca gtagcatcta cagacgccac
    87361 catcccctgt ttcgcgcgta ctggatgaaa gacatcttcc tccacgccat aagaaacagg
    87421 aggcgcggcg gcggcgctaa aacaacttcc tgagagcaga gcagcaatgg ccacccggcg
    87481 taaaaacgtc ggttttatca tcgttattct ccagttatgt gtggggcaac cccagctaag
    87541 cctggtatat aactctgaat taatcatcgt tttgccggga agcgagtaaa cttaaagaat
    87601 atcttcagag gaggatacgc gatgaaacga cttctgattc ttacggcact cctgccgttt
    87661 gtcggctttg cacagcccat taatactctg aataacccta accagccggg gtatcagatc
    87721 cccagtcagc agcggatgca aacccaaatg caaacgcagc aaatccagca aaaagggatg
    87781 ctgaatcagc aactgaaaac gcaaactcag ttgcaacagc agcatttaga aaaccagata
    87841 aacaataatt ctcagcgggt gttgcagtca cagccggggg agcgaaatcc cgcccggcag
    87901 caaatgctgc ccaacaccaa cggcgggatg ttaaacagca accgtaatcc ggatagttcg
    87961 ttgaatcagc agcatatgct gccggagagg agaaacggcg acatgctgaa tcagcccagc
    88021 acgccgcagc ctgacattcc gttgaaaact attgggccgt aaagttcgga ccaattacat
    88081 caatcgcatc ggtacagatg caatccacgc cccagcgcag caactctgcc gcgcgctggg
    88141 gtttgttgac ggtatacacc agaatccgca gtccggcgtc tttcaactgc atcactcgcg
    88201 ctttatcgag caacttatga ttgagatgaa gagagacgca gcccagccgc atggtcagtt
    88261 cgcgccagtc gtcgcgccac tcatccagca ataaaccacg cggcagttcc ggtgccgcca
    88321 attgtgcggc ttctaaagca tcaatctcaa acgatgacag cagcggcggc gtcataccgg
    88381 cccacagttg gcgtgccgcc agcgccacca ttttgcccgt taatggtcca gtgccagtgg
    88441 tgggtttgat ttcgatattc gccatcatcc cgtgttcgcg acagcgttcc gccacctgcg
    88501 aaagcaacgg cagcggctca cctttaaacg ctttgctgta ccaactgccc gcatccacgc
    88561 gcagtaaatc ctgccagttc agttcacccg cgacgcccca gccgttgctg gtgcgttcga
    88621 gattgtcgtc atggagcagg aagatctcgc catctttcga taacttcgcg tcaaattcga
    88681 tcatcttatg accgtatttt gccccgacgt cgattgccgc cagggtgttt tccggggcca
    88741 gcttaccgcc gccacgatga gcgacgatgc ggggataagg ccagttactc atactcgttg
    88801 tcctgtttca ccatcaaaaa gatgcagctg attttccgcc agatgcagcc acagcgtgct
    88861 gcctgccgtc gggcgctcct gatgcgccag tcgcaccacc agcttctgct cgccccagcg
    88921 tccgtgcgcc aggttatctg cgccgaggat ctccagcgtg tccatcacca gcggcacacc
    88981 accttctgcc tgcgagctta gcgctatatg ttccgggcga atgccgagag tcattttacg
    89041 cccggcatac tgacggtagc caccgtttag cggtagcacg ataccgccgt ccagttcgaa
    89101 atgcgtgcct tcattattca cgcggcctgt cagcaggttc atcgccgggc tgccgataaa
    89161 actcgccaca aacaggctgg cgggcttttc gtagacttcc accggcgtgc caatctgttc
    89221 ggcaacgccg ccgttcatca ccattactcg ctgggcgagg gtcatcgctt caacctgatc
    89281 gtgagtaacg tagagtgaag tcgttttcag gcgacggtgc agctgttgca gctcaaggcg
    89341 catctgcacg cgcagcttgg catcgaggtt agagagcggc tcatcaaaca ggaacaccgc
    89401 cggatcgcgc acaatcgcgc gacccatcgc cacacgctga cgctgaccgc cggatagctc
    89461 gcgcgggcgg cgcttaagca ggccatccag ctccagaatg cgcgccgctt ctttaacgcg
    89521 ctcggctatt tgctgcttgc ccatgccgcg aattttcagc ccccacgcca tgttttcttc
    89581 gacgctcata tgcggataaa gcgcgtagtt ctggaacacc atcgcaatcc cgcgatcttt
    89641 cggctccatt tcggtcacgc gctggtcgtt gatccagata tcgccttccg tcacccgctc
    89701 cagcccggca accatgcgca gcagcgtcga ttttccgcag ccagacggcc cgaccatcac
    89761 gataaattcg ccatccgcca catcaagggt cagtggttta atcacctggg ttttgccatc
    89821 ccagcttttg gttactgcct gtaatttcag tcctgccatc ttatttctca ctatcgacca
    89881 ggccgcgcac gaaggcacgc tgcatcacta aaacaatcac caccggaggg ataagcgtta
    89941 acaacatcgc cgccattacc gagttccatt ccgtggtgcc ttcgccagtg gcgatcatcc
    90001 ctttgatccc tgccacggtg gtgccgagat caacgtcggt aataatcaac aacggccaca
    90061 aatactgatt ccagccgtag ataaaggtga tcacaaacag cgccgccaga ttagttttgg
    90121 agagcggaaa aacgatgtcg caaaagaagc gcattggcga tgcgccgtcg atccgcgcgg
    90181 cttccaccag ctcatccggc agcgtcataa agaactggcg gaacaggaaa gtagcggtcg
    90241 ccgaggccat cagcggcagc gttaaaccgg cgtagctgtc gagcatctgc aggttggcga
    90301 tgacttccac cgtcgggaag atacgtactt caaccggcag catcagggtg ataaaaatca
    90361 tccagaagaa gaggttacgt agcggaaaac gaaaccagac aatggcaaat gccgagagca
    90421 tcgagacggt aattttgccg agcgtaatgc tgaacgccat cacaaagctg ttaagcaaca
    90481 tccgccagaa cggcgcgcta ttcgtgccta ccccgtttac ccagatgttg tggatgtttt
    90541 ccagcagatg cgtgccgggg attagcgtca tcggcgcggc gtagacggcc tgtttatcca
    90601 gcgttgccgc tacaaacgcc acgtacagcg ggaagaggat caccgcgatc ccgaggatca
    90661 gcatggtatg gctgaatatc gtcagccacg gacggttctc aatcattggt aacgcacctt
    90721 gctttcaaca tagcggaact gcaccaccgt cagcacgatg acgaggaaca tcaacaccac
    90781 cgactgcgcg gcagacgaag ccagatccag tccggtaaag ccttcgcggt agatcttata
    90841 aatcagcgtc gtggtggcct gaaccggccc gccggacgtg gcggcgtcga tcaccgggaa
    90901 ggtgtcgaag aaggcgtaca ccaggttcac caccagcagg aagaaactca ccggagcgat
    90961 aagcggcaga gcaatcttaa agaagcggcg aatcggccct gcgccgtcaa tagctgcggc
    91021 ttcgatcaac gaacggggaa tggattgcag cgcggcatag aagaacagaa agttgtagct
    91081 gatttgcttc cacaccgagg caaacaccac cagaaacatc gcctgaccgc tgttttgcgc
    91141 gtggttccag tcgtagccga actcagcgag aaaatgggtg atcagcccgc gaccggggtt
    91201 aaacaggaag atccacaata cggcggcaac ggcgggagcc acggcatacg gcagcagcat
    91261 taaggtttga tagaaacggc tgccgcgcac gatgtactcc accagcgccg caaagaacag
    91321 cgataccagc aaaccgctga cggtgacaaa ggtgctgaat tttatcgtcg tccagaagga
    91381 gtcgatatag tagctgtcat gaaacagcgc gacgaagtta tccaggccga caaactggct
    91441 ggagaaacca aatggatcga cgctttgtag cgagtaccac aacgcttcgc ccgcaggcca
    91501 gataaaaaag ataacggtga tgatgagctg cggcgcgacc agcagataag gcagccagcg
    91561 cgagcggaac accggacggg atgatgacat tacggttaat tcctgaactg tgccggatgc
    91621 gcttcgctta tccggcctac acgcgatgcc aggtcagata agcgaaacgc atccgacatt
    91681 acactgatta agacttcgtc gatttctcaa agcggcgcag taactggttc ccacgctcaa
    91741 cggcggtatc cagtgcctgc tgcggtgtct tcttaccggt ccacacgctc tccagctctt
    91801 catccacaat cacgcggatc tgcggcatgt tgcccagacg cagccctttg gtgaacggca
    91861 acggcggctt gttcagcatc tgacgtgtcg cggtatccgc ccctgggttt ttctcataaa
    91921 agccctgctc acgggtcagg tcatacgccg ctttggtaat tggcagataa ccggttttct
    91981 gatgccactc agcagcgttt tctggcttcg cgaggaaatc gaggaacttc gccacaccgg
    92041 tatacgtttc tttatcttta ccctgcatca cccacaggct ggctccgccg ataatggcgt
    92101 tttgtggcgc atctttcgca tcggcgtcgt aaggcatcat gcctacgccg tagttgaatt
    92161 tggcgtactc gcgaatgttg gcaagagaac cggaagaggc ggtggtcatc gcgcaatcac
    92221 cgttatagaa cttctcggtg gattcatcct tacgcccgac gtagctgaaa tcgcccttct
    92281 tgttcatctc ttcgagcatg gcgatgtgtt tcacctgctc cggcttgttg aactccagca
    92341 ccgcgtccgt gccgtcaaag ccgttgtttt tgctggcaaa cggcagaccg ttccaggcgc
    92401 taaagttttc cagttggatc cagccctgcc agccgctggc gtagccgcac ttcataccgg
    92461 aggctttcag ttttgcggaa tagtccgcca gatcctgcca ggttttcggc ggctgttccg
    92521 ggtctaaccc cgctttcttg aaggcgtctt tgttgtaata gagaacggga gtcgagctgt
    92581 tgaatggctg ggagagtaag tggcccgttt tgctgtcgga gtagtaaccg gaaaccgtcg
    92641 gcacaaactg cgactcatcg aattgaatcc ccgcctcttt aaacacgtca tacaccggtt
    92701 taatggcttt cgacgccatc atggtggcgg tgccaacttc ataaacctgc aaaatagccg
    92761 gtgcgttgcc ggtacgaaat gcggcaatcc ccgcgcttaa attctgttcg tagttgcctt
    92821 tataggtcgg tacaattttg taatccgggt tttcggcgtt aaaacgttgg gccagagaat
    92881 ccacctcttt acccagttcc ccttccatag aatgccagaa cggaatggtc gtcactgcct
    92941 gtgcattccc cattaacgcc agtccgagcg ccagtgctga agctgtataa tgtaacggtt
    93001 tcatcgttta tctctcttgt tgtaccgaat gcgcgaattc acgcgtttta tgctcgcggg
    93061 gtaacatgac atgctcgaat tacagaaaaa taactttttt gttacatttg taagatagta
    93121 aggtgtcaga aagatgacaa ggcggtgacg gcgtgggtga gggaaaatgg gagatggggc
    93181 acggataagc ggaaaaatag agggtctgaa tcaaactcta cagattactc atcgtttcat
    93241 gccggatgcg gcgtaaacgc cttatccggc ctacaagatc gtgcaaattc aatatattgc
    93301 aacccaccca gtaggcctga taagcgcagc gcatcaggca attttacatt tgtcacctgt
    93361 ctcaaagaag tcttttgact ccttatcaat caacgtgtta ttacccgcct aaatacgcac
    93421 ttctcaccgc ttcgttcgcc agtagtgcat caccagtatc ggagagcacc acatggccgt
    93481 tttccagcac gtagccgcga tccgccagct ttagcgcctg gttggcgttc tgctcgacga
    93541 ggaagatagt catcccctgc tcgcgcagct gctcgatggt gtcgaaaatt tgctggatga
    93601 taatcggcgc aagaccgagc gatggctcat caagtagcag caaacgcggg ttgctcatca
    93661 gcgcgcgacc aatcgccagc atctgctgtt caccgccgga catggtgccc gcccgttgaa
    93721 cacggcgctc atggagacgt ggaaacagct catacaccca ctttatgcgc tcctgaaact
    93781 gatcacgttc ggcaaaaaag ccgcccatcg ccaggttctc ttccaccgtc atccgcgaga
    93841 agacgcgacg cccttccggg acaatcgcta ccgcttcgcg catgattttc gccgtttgcc
    93901 agtcggtaat gtctttatca tcaaacacaa ttcgcccgct ggtggcacgc ggatcgccgc
    93961 ataacgtgcc gagcaaggtg gttttccccg ccccgttcgc gccaatgagg gtgacaatct
    94021 cgccctgatt gatatgcagg ctcacctcat gcagcgcctg gattttgccg tagtgggcgc
    94081 tgactttgtc aaaggacaac atgacttttt ccatcttatg cctcacctaa ataggcacgg
    94141 atcacgtccg ggttattacg gatttgctcc ggcgtaccgt ttgccagtgg cgtcccctga
    94201 ttgaccacgt aaattcggtc cgaaattccc atcaccagct tcatatcgtg ttcaatcaac
    94261 aagatagtgg tgttgtgatg attgcgcagt tcggcaatca gctcatccag ctctttcgtc
    94321 tctttcgggt taagacctgc cgccggttcg tcgagcatta aaatctccgg ctgcgtcacc
    94381 atgcagcggg caatctccag acggcgttgg tcaccatagg ccaggttact cgcctgacgg
    94441 ttggcgtgtt ccagcaaacc aatgcgctca agccaggtgg cggcgcggtc gagcgcttcg
    94501 ctctgggcgc gacggaaaga tggcgttttc aacaggccag agaacagccc ggttttcagt
    94561 tgctgatgct gcgccaccag caggttttca attaccgtca tttcacggaa cagacgcaca
    94621 tgctggaagg tacgcaccac gcccatgcgg gcaatttgct gccccggtaa accttccaga
    94681 tgctgatcac gcagtaaaat agtgccgccg gtgggtttgt agaatccggt caggcagtta
    94741 aaaaccgtgg tttttccggc accgttaggg ccgattaacg agacgatctc ctgcgggtac
    94801 agttcaagat tgacgttgtt caccgccagc aagccgccga agcgcatcat cagaccgtta
    94861 acagataata atggctgact catgcctgct ctcctttcgc tgcgccgttt ttcagcttca
    94921 gttgcgggcg cgtcatgggc agcaagccct gcggacgcca gatcatcatc agcaccatca
    94981 aaccaccgag cattaacatg ctgtattcgt tgaaatcacg catcaactcg cgcgacacca
    95041 ccagcaaaat tgccgccaga atcaccgcaa attgcgagcc cataccgccg agcaccacta
    95101 tcgccagcac aaacgccgat tcggcaaagg tgaaggattc cgggctgaca aagccctgac
    95161 gcgcagcaaa cagcgttccg gcaaaaccgg caaacgcggc actgatggta aaagcggtca
    95221 gcttgatacg acgcgggctt aaacccagcg aacggcaggc gatctcgtct tcacgcaacg
    95281 cttcccacgc acgccccagc ggcatccgca gcaggcggtt aatgacaaac agacttaata
    95341 ccaccagcag caacgccacc aggtagagga agatgacacg atcggaggga tcgtatttca
    95401 ggccaaagaa attactgaac gtgtcccagc cgccttcacg agcggtacgg ctgaactcga
    95461 gtccgaagag tgtcggtttc gggatctgac tgattccgtt cgggccgccg gtaatttcgg
    95521 tattattgag caacaatatg cgcacaattt cgccgaaacc gagggtaacg atcgccagat
    95581 agtcaccgcg caagcgaagc accggaaagc cgagcaggaa gcccgccgcc gcggccatta
    95641 acccggcaat cggcagacag gtccagaagc ccaggccgta atagtgattg agcagcgcaa
    95701 aagtgtaagc gccgatggcg taaaagccgc cgtaccccag caccagcaga ccagaaagac
    95761 caacaaccac attcagcccg agaccgagga taatgtagat catggtcaga gtggcgatat
    95821 ccaccgtccc acgtgacacc ataaacggcc acgccaccgc aagcaccagc agcgccacga
    95881 ggaacagttt ctgcttcacc gtggagccat caatcgcggg cagaataaac ttcggtccgg
    95941 aaacactttt caaccctttc tggaaagcag gtcgcaaaag ctgaaagaaa aagaccaccg
    96001 ccgtgccgat aaacacccac tgccaacgga tgtctgaagc cgtgtcgacc accagtttgg
    96061 tgccatccag ctccagttgc acgcccataa agacgcccgc caggacaaaa aacatcgcgg
    96121 cagagagcag cgccattgca atatgcatcg gtttcatact ttctctacct ccgggcgacc
    96181 cagaataccg gtcggcatca ccagcagcac cagaatcagc agggcgaatg agaccacatc
    96241 tttatattcc gtactcagat aggcagaaga gagcgcctcc gcaatcccca gaatcagccc
    96301 gccaatcatc gctcccggaa tgctgccaat cccaccgagc accgcagcgg taaaggcttt
    96361 catcccggcc ataaagccga tgtaggggtt aattacgccg tagaactgac cgagcagcac
    96421 acctgccacc gccgccatcg ccgcgccaat cacaaaggtc agcgcaatca ctcggtcggt
    96481 gttaatgcca agcaggctcg ccattttcag gtcttccgcg caggcacgac acgcgcgccc
    96541 catacgggaa tagcgaatga aaaccgtcag cgccagcatg gcgaggaagg tgacaatcca
    96601 gatcaccgcc tgcatggtgg taatagaggc agagaagttt tcgctatgcc ccaccaccca
    96661 ctgaccgtta aacagactgg gcagcgccac gtcacgcgaa ccttcagtca ggctaacgta
    96721 gttttgcagg aagatggaca tgccgattgc cgagatgagc gcaatcaggc gcttagagtt
    96781 acgcaccggg cgataggcta cccgttcgat actccagccg taggcgctgg cgatgacgat
    96841 tgcgccgacg aatcccgccg ccaccagcag ccagccggta tcaatgccca tcatcatcag
    96901 cgcggcgatg atcataaatg agacatagct gccgatcata taaacctcgc cgtgggcgaa
    96961 gttgatcatg ccgataatgc cgtaaaccat ggtgtagccg atggcaatca gcgcgtaggt
    97021 actgcccagc gtgacgccgt taaacatctg ctgcaagaaa tataaaaact gctcagacat
    97081 aaggtaacct ttctaaaccc gcccgttttt cacgggcggt gggatgatca cttggctgcc
    97141 gtggatgaac cgtcggcgtg ccactggaag acaccaaaat caaatccctt aagatcgcct
    97201 ttttcatccc agttcagcgg cccaatcacg gtgtttgcac cgttagcttt taaatctttc
    97261 accagcgcca gcggctcatc gctgccagta cgctcaaggg cagtcgccag agattgcacc
    97321 gccgcgtagg tgatccagac ataaggcccg gacggatctt tcttgtctgc tttcagcgca
    97381 tcaacgatgc cctggtttgc cggatcctgg tcatagcgtt ttggcatagt gaccaacatg
    97441 ccttcggcag catcgccagc aatattcgac aatgatgcgt tacccacacc ttccggcccc
    97501 ataaactgag ttttcaggcc aacggaacgg gcctggcgca gcatctgccc catttccggg
    97561 tagtaaccgc cgtagtaaac gaagtcgatg ttttcttttt tcaggcgggc gatcagcgcg
    97621 gagaaatctt tctccccggc ggtaataccg tcgaagaaga cgacgttggc gttagccgct
    97681 ttcagcccgt cctgcaccga acgcgccagc ccttcgccat actgttgttt gtcgtgaatg
    97741 atggcgatgc gctggggctt caccgtctca agaatgtatt ttgccgccgt aggcccctgg
    97801 gaagagtcca gcccggcagt acgcataatg tgttgataac cgcgttgggt cagctccggg
    97861 ttggtcgctc ccggcgagat catcagaata ccttcgtctt cgtagatatc tgacgcaggt
    97921 tgggtagaag aagaacacag atgaccaata acgtatttaa tgccgtcgtt aacgattttg
    97981 ttggcgaccg caacggcttg tttcgggtcg caggcgtcgt catattccac gcccaccagc
    98041 ttatcgcctt taattccccc tttggcatta atgtctttaa tcgcctgacg cgcgccgtta
    98101 aattccatat cgccccactg ggcaatcggg ccggacatcg cgccgacaac ggcaacttta
    98161 atatcgtcag ccatagcggt gtgtgaaatt gccagtgcaa tcatccctgc gatgatagtt
    98221 ttcgcattcc gtttcatagt caaaaatccc cattcgtgat gttgtgttgc tttgttttta
    98281 tgtgttaaca aatcagactg ttcttttttt atactgcact atttttgcct gtctgattaa
    98341 gggtttagcg cagtattttg tgataatagc gattaaaatc cctatttttc agtcgattaa
    98401 gaacagataa tattctgaat ttattgatag ataaacagaa aaaagcgccg ttgtcagcat
    98461 aaaataacgg tacaaagagc ggaataattc actaccattc aggggattat gctgggtatt
    98521 tttcattctc taatgtttta actttgtaat tattgctgtt aaaaaattaa tcacctgcca
    98581 aaagaaataa aaaagagaaa gcctccgatt aaattatttc gctacactgg ttccactttt
    98641 gtgatttaca cgggttcccc atgaagctga ccatcattcg attagaaaac tttagcgacc
    98701 aggaccggat tgacctgcaa aagatctggc cggagtattc cccttcctct ttacaggttg
    98761 acgataacca ccgtatctac gccgcgcgtt ttaacgagcg cctgctagcg gccgtgcggg
    98821 tgaccttaag cggcaccgag ggagcactgg attccctgtg cgtgcgggaa gtcacccgcc
    98881 gtcgcggtgt ggggcaatat ctgctggaag aggttttgcg taacaatcct ggcgtttcat
    98941 gctggtggat ggcggatgca ggcgtggaag atcgcggtgt gatgacggcg tttatgcagg
    99001 cactgggatt tacggcgcaa cagggcggct gggagaagcg ttaatcgctc tgtgtgaaaa
    99061 gcagttcgat ggatttggtt gggaaaaata acgcattttc gttttctccg accaacggga
    99121 taaagcccag cgattgataa aacgtatggg cttttttatt cagcgcctcg acaaaaagac
    99181 cgtgaatacc taccgctaaa gaggctgacc agacgacttt catggcatga gcaaccagtg
    99241 ttgctcccca tccctgcccc tgtaatgaac gatcaattgc cagacgccca agagtaacgc
    99301 tgggaatatt tttgtaggga atttttttct gtttcgattt cgagggcaat gcggctcgtt
    99361 caaaacaact gccgcataat gtgtaatatc ccagcacctg acgttctgga gtgttgcgac
    99421 aaaggatata cgctcgcaga attttgttgc gatgttgacg aacgagatgt gtcgtcagaa
    99481 agagatttaa cgcttcctcg ccgcagtcga atcgctgtag atcataatct gcatcatcgg
    99541 tcagaatctc tatcgtcaga tcatccataa attattacat tccctgaaga cgttttgccg
    99601 cacgttttag cttttcacca ggtgacggtg gattactcag cgcatccatc acccgcgtcc
    99661 aggattcctc attgaggatc acccgccgat gctgttcaat cacttccgca gcccgctgcg
    99721 aagcgctgtt caacataaat tggctgacgg actgattaga tatcgctgcc gcttcctcaa
    99781 tcatactttt gtcatcgtca gttaaacgca gatcgatacg ctgcttttta acagcagaca
    99841 tgtgaactct cctgctccgt ataatatgta cggtatgtca ccgtacatta agcatatcct
    99901 ggattgttta atctttctac aaccatttcg acaaacacaa aaaaacccga tggcagttca
    99961 gcgcatcggg cattttcatt tatctatctg ttacttcgca tcggttgccg tgccgttggc
   100021 gtgccagtca aatacgccga actcaaagcc tttcagatcg cctttctcat cccacgtcag
   100081 cggccccatc acggtatcaa ccgagttcgc tttcaggtat ttggcgattt cagccggatc
   100141 gtcagactga ttcaggcccg cctgcaaaga ttgcagcgcg gcgtaggtgg tccagacgaa
   100201 cgcgcggctt gggtcctgtt ttttcgcttt gatcgcgtca acaatgggtt tgttcgccgg
   100261 aacctgatcg tagttcttcg gcttggtcac cagcagccct tccgctgatt cgcccgcaat
   100321 gttagacagc gaaacgttag ccacaccttc cggccccata aactgagttt tcagccctgc
   100381 cgcgcgtgcc tgacgcagga tctgccccat ttccgggtga taaccgccgt agtaaacgaa
   100441 gtcgatattc tcttttttca gacgcgccac cagcgtagag aaatcttttt ccccggcggt
   100501 gatgccatca aagaacacca cgttagcatt gcctttcttc aggccgtcct ggaccgcacg
   100561 cgccagacct tcgccgtact gctgtttgtc gtggacgata gcaatacgct gcggtttcac
   100621 cttctcaaga atatatttcg ccgccgtcgg cccctggtcg gagtccaggc cggtggtgcg
   100681 caggatcagc tgatagccac gagcggtcag ctctggtgcg gttgccgctg gggtgatcat
   100741 taaaatgcct tcatcttcat agatgtctga cgctggctgc gttgatgaag aacagaggtg
   100801 accaatcaca tatttaatgc cgtcgttaac gactttgttc gccaccgcaa ccgcctgttt
   100861 tgggtcacag gcatcgtcat atttcacgat ttgcagtttg ttgcctttaa tgccgccttt
   100921 agcgttgata tccgcaaccg cctgctctgc gccagtaaac tcctggtcac cgtactgggc
   100981 caccggaccg gacattgcgc ccacgaccgc gactttaata tcttctgcca gagccatatt
   101041 gctgaatgcc agcgcgatac atcctgccag taacgcttta cccttcatgt tcatcctgag
   101101 gttccccatt cttctggtta ttacgtgtgt tgtgatgttg ttgttcagca ctttatttcg
   101161 ttttatgcat gactacccgt gctttagcag catactctgc taaaacatac ccgattttta
   101221 tgatattgga atagctattt tgacagttta ttaacaatct gcgtggggat tagcgttttg
   101281 ccggaggggg aaactgatta ttgcagaggc ccaaaaaaca aaaaccccgg attctcatcc
   101341 agggttctct gcttagtagt agaaattacg cttcaatggc agcgcgaagt tttttcatcg
   101401 cgttcttttc cagctggcgc acacgctcgg cggaaacacc gtaacggtca gccagttcct
   101461 gcaacgtgga cttgttgtct tcgtccagcc agcgcgcacg gatgatgtcc tggctgcgtt
   101521 cgtccagacc ctgcatcgcg tcggtcagac ggtttgccgc ctgctcttcc cagttatcat
   101581 cttcgatgcc gtcggcaaag ttagatgatt tatcctgcag atagagcacc ggtgccatcg
   101641 gctggctgtc ggaatcgtcg tcggaagaca ggtcaaaggt catgtcctgt gccgccatac
   101701 gtgattccat ctcacgaacg tctttgctgg ttacgcccag ttcacgggcc accatttcga
   101761 cttcatcctg gttaaaccag cccagacgct gcttggtttt acgcaggttg aagaacagtt
   101821 tgcgctgcgc tttggtggtc gcaactttga cgatacgcca gttacgcaga acgtattcgt
   101881 ggatctctgc tttgatccag tgaacggcga aggagaccag gcgcacaccc acttccgggt
   101941 tgaaacggcg cactgctttc atcaggccga tgttaccttc ctgaatcaaa tccgcctgtg
   102001 gcaggccata gcccgcataa ttacgagcaa tatgaacaac aaaccgcagg tgagacagga
   102061 tcagcgtttt agctgcttcc agatcgccat ggtaatgcag cttttcagcc agcgcccgct
   102121 cctcgtcagc cgacaacatc ggccacgcgt tagctgcccg gatgtaggaa tccaggttgc
   102181 caactggggc taaagctaaa ctttgcattt tgtcagtcat tcaaatcctc tcaatcgata
   102241 tcttctggcg cttcagtggt agcaacaact gtgccagagc ttaagagcaa cgaggttatc
   102301 attcactgtt ttatcagacc gtgattttat ccacaagttc aatgcaagct tgtgaataaa
   102361 ttacgcacaa aatgtgacat agagatgaaa taccgggaag agacaacggg atctctttcc
   102421 ctgctacgga acccattgca gggaaagagt ataacacgct tttattattc aggcgtaaag
   102481 tggcgtaaat gttgtaccgt ggcaagccac gctgccaccc agccaatcat cgagcatacc
   102541 agcagcaata gcaggcattc atcgaacgat aaaccattga tatcaaactt cgttccgaaa
   102601 acctgtgcca cttccgcaac cgccgatgac aatcgcagca ccagaatttc tgacaaaatt
   102661 aatgacaaca atgcgccaga aaatcccagc agtgcgccac catacaggaa cgggcgaagg
   102721 atgaaaccat ccgtcgcacc aatcagtttc tgcacgttga tggagtcacg gcgagcaaag
   102781 atgctcagac gtacgctgtt gccgatgacg aggaacactg ccgccaccat caacacgccg
   102841 atcatcgccg aaacgcgccc gaccagcccg gtcaacgccg ccagacgggc aaaccagctg
   102901 tcatccatcc gcacttcgtc aatgccgtta atctgcgtga tacgatcacg cagcgtattg
   102961 agtgattccg tcccctgaaa atcgagtttc gggatcacca ccgccactgc cggaagcggg
   103021 ttttcttcca gcatatccag cgcaccacca aaaccagacc agttacggaa ctcgcccagt
   103081 gcgtcttcac gagaaagata gttcactttc tccacgcctt gctcggcctg caactgtgcc
   103141 accacgcccg cagcggcgtc atcgtccagc gttttttgca agtaaacagt gatttgcggt
   103201 gacggataat actgcgtcgc cgcctgatta acgtttttgt acaccatata acagacgctg
   103261 ggcagcgtca gggagatggc gataaccatc accgttaaaa acgtggcgaa cggtttgctt
   103321 ttcagatcct gcaatgcgcc gtggaaggca tagcgcacct gttcgttgaa aacgttggtt
   103381 ttgcgattta ccggttttgg cgaggatttc gcgcgtttcg gtgcgttacg accgccgtcg
   103441 cctgagccgc cgaccgattt acggaagcga tccagacgcc cgccaaactg ccgaatatga
   103501 ttgattgcat cgcgcttatt catggcccac gcctccatgc aagtgaccat cgctcagggt
   103561 gagcatgcga taggaacgcc gcgaaatcag gttgatgtcg tgcgttgcca tcaatacggt
   103621 taccccaacg cggttaaact cttcaaacag acgtagaatc ccttccgaca acgcgtcgtc
   103681 caggttacca gtcggttcgt ccgccagcag taccgcgggc ttgttcacca ccgcgcgggc
   103741 aatgccaaca cgctgttgtt caccgccgga aagctgaata gggaagttct tcgctttgtc
   103801 cagtagcccg actttatcca gcgccgccga cacccggcga cgaatatcgt caccgctggc
   103861 accggcgata atcagcggga tcgccacgtt atcgtagaca gtacggtcca tcagtagatg
   103921 gtgatcctgg aaaatcatgc caatctggcg gcgcagaaac ggaacttcac ggtttttcag
   103981 acgcgtgatg tcatggccgc taaaccagat tttcccggcg ctgggccgct caatcccaca
   104041 gatcagcttc aggagggtac ttttccctgc gccggaatga ccggtcagaa acgccatctc
   104101 acccggctgc atatggaacg taacgccctg cagcgcctgt ctcccaccga gataagcctt
   104161 gctgacatgt tcaaagcgaa tcattgttaa tcctctcggg caaaaagtgc ctctataaag
   104221 tcgtccgcct taaacggacg caaatcctca atacgttcgc cgacaccaat gtagcggata
   104281 gggataccaa actggtcagc caccgagaaa attaccccgc ctttcgccgt gccgtccagt
   104341 ttcgttagcg tgatgccggt taagccaaca gcttcatgga acagtttggc ctggcttacc
   104401 gcgttctgcc cggtgctggc atcgatagtc agcataactt catgcggcgc ttcaacgtcg
   104461 agtttcttca tcacgcggac gattttcttc aactcttcca tcaggtgcga tttgttctgc
   104521 aggcgtccgg cggtatcggc aatcaggacg tcgatattac gcgctttagc ggcctgaatg
   104581 gcgtcgaaga taacagaggc ggaatccgca ccggtatgct gggcaatcac cggaatattg
   104641 ttgcgctgcc cccagacctg aagctgttca accgctgctg cacggaaagt atcgcccgcc
   104701 gccagcatca ccgatttacc ctgctgctca aactgacgcg ccagcttacc aatcgtcgtg
   104761 gttttaccca caccgttgac gcccaccatc aggatcacga acggcgtttt accttcaaca
   104821 ttcagcggct catcgacttt cgccagaatc tcgcccatct cttctttcag caggccatag
   104881 agggcctcgg catcacgaag ctgcttgcgg gatgcgcctt ccgtcagatt ggtgatgatt
   104941 ttacgtgtgg tttccacgcc cacatcggcg atcaacagct gttcttccag ctcttcaaac
   105001 agatcatcgt cgattttttt accgcggaac aggctgataa atccggaacc gagattttct
   105061 ttggttttta acaggctgcg tttcaggcgc gcgaagaaac cttctttggt cggtttttcc
   105121 tgctcctgag cgatttcttc caccggctgc tcttctaccg gaggaaccac catcaccgcc
   105181 tcttctgccg cttcggcagc cagcgcctgt gcttccagct cttcgtcggt gatttcttct
   105241 ttagccgctt cttcttccgc cgcttcgaca atctctacgg tttccgcttc agcctgccac
   105301 tcttctggcg aaacctcttc ggcgttgacg tcttccggca acggcagctc ttcacgctcg
   105361 atagccactg gctccggcgt ttcttctacg accggttccg gctgtgcaac gacttccgct
   105421 tcaggctgcg ctttttcact ttcagcaacc tgttcagtga cttccacaac gtcggcagca
   105481 aaagtttccg cctcggcttc agtatgcgcc tgcggctgct cttcgacggc ttgttcagaa
   105541 gccttcacag gctcttgcgc ctgaacgatt tcttctacaa ccggttgttc attctgaact
   105601 tctgtctctt tttccggggt ctgctctttt tgaccaaagc ccagccagga aaaaaagcca
   105661 cgtttttttt ctttcgccat ttgcgactac actcctcgct gttgcttcat ggcacagcgt
   105721 taacgctatg tacatagcag ctaaaaaaat gatgaaatag tctatcactt aacttaattc
   105781 acatcaccgc ctgcaagtat gtgttatctg gcggattgag caatttatca tgaaaaaacc
   105841 gaatcattcc ggcagcggcc aaatccgcat tattggcggg cagtggcgag gccgtaaact
   105901 cccggttcct gatagcccag gtctgcgccc caccaccgac cgcgtacgcg aaacgttgtt
   105961 taactggctg gctccggtca ttgttgacgc ccaatgtctg gattgcttcg ccgggagcgg
   106021 cgcgctgggg ctggaagcgt tatcgcgcta cgctgcgggg gccacgttga ttgagatgga
   106081 tcgcgcagtt tctcagcagt taattaagaa tctggcgaca ctaaaagcag gcaatgcacg
   106141 cgtggtgaac agcaacgcaa tgtcattcct ggcgcaaaaa ggcacaccgc ataatatcgt
   106201 gtttgtcgat ccaccgttcc gccgtggctt gttagaagag acgataaatt tactggaaga
   106261 taacggctgg ctggctgacg aagccctgat ttatgtcgaa agcgaagtcg aaaacggtct
   106321 gcccactgtt ccggcaaact ggtcattaca tcgggaaaaa gtggcgggtc aggtggctta
   106381 tcggctgtat caacgcgaag cacaaggaga aagtgatgct gattaatatt ggtcgtttgt
   106441 taatgctctg cgtttgggga tttttaatcc tcaatctggt gcatcccttc ccgcgcccgc
   106501 tgaatatttt cgttaacgtg gcgctgattt ttaccgtgct gatgcatggt atgcaactgg
   106561 cgctattgaa atccacttta ccgaaagatg gcccgcagat gactaccgcc gaaaaggtgc
   106621 ggattttcct tttcggcgtg tttgaactgc tggtctggca gaagaaattt aaagttaaaa
   106681 aataacctta ttgttcgcct acaaagctga caaagcgcgt tcctttatag ctcagcgtac
   106741 ctttatcgcc cactgtcagg gcgtggtact gctgggcgtc gaggcgaaac gtctgctcca
   106801 ttcctccgct ttgcggtttg aagcttgcct catagcgcat actggtgcct gccggagtca
   106861 cttcctgctg gcgcgaacgg cgatcgttaa tcggtttttc ccgcttgttg ctcaccacca
   106921 ccagcttttg ctggagcgga gccatatcat tatcagcttt ttcccgtcgc tgttgcataa
   106981 aacgaaacga tgcggcgacg acaattaagc caatgataac aataaagaaa agaggtggtt
   107041 tgctcatctt tatccctcat cggaaaatgc ggaaataagc ataccctgcc agttatggtg
   107101 ttgtcatccg tccaccctcg ccactaaact ggaagcaaga ccgtaggcat tccgcttacg
   107161 aaaaaataac gaattcaagg aactaagatg ctttggtcgt ttatcgctgt ctgtctttcc
   107221 gcatggctat ctgtggatgc atcgtatcgt gggccaacct ggcaacgctg ggtgtttaaa
   107281 ccgttaaccc ttcttctcct gctgttactg gcctggcaag cgccgatgtt cgacgccatt
   107341 agctatctgg tgctggcagg gctgtgcgcc tcactgctgg gcgatgcgct aaccctgttg
   107401 ccacgtcaac gtctgatgta cgccatcggc gcgtttttcc tctcgcacct gctgtacacc
   107461 atctatttcg ccagtcagat gacgctctct ttcttctggc ctctgccact ggtgttgctg
   107521 gttctgggcg cgctgttact ggcgattatc tggacgcggc tggaagagta tcgttggcct
   107581 atctgcacgt ttatcggcat gacgctggtg atggtgtggc tggcaggtga actgtggttc
   107641 ttccgtccga ccgctccggc actctctgcg tttgttggcg cttcattgct gtttatcagt
   107701 aactttgtct ggctgggtag ccactatcgc cgccgcttcc gggcagataa cgcgattgct
   107761 gcggcctgct actttgccgg tcatttcctc atcgtccgct cgctgtatct ctgataaaac
   107821 ttgactctgg agtcgactcc agagtgtatc cttcggttaa tgagaaaaaa cttaaccgga
   107881 ggatgccatg tcgactcctg acaatcacgg caagaaagcc cctcaatttg ctgcgttcaa
   107941 accgctaacc acggtacaga acgccaacga ctgttgctgc gacggcgcat gttccagctc
   108001 gccaacgctc tctgaaaacg tctccggcac ccgctatagc tggaaagtca gcggcatgga
   108061 ctgcgccgcc tgtgcgcgca aggtagaaaa tgccgtgcgc cagcttgcag gtgtgaatca
   108121 ggtgcaggta ctgttcgcca ccgaaaaact ggtggtcgat gccgacaatg acatccgtgc
   108181 ccaggttgaa tctgcggtgc aaaaagcggg ctattccctg cgcgatgagc aggccgccga
   108241 cgagccacaa gcatcgcgcc tgaaagagaa tctgccgctg attacgctaa tcgtgatgat
   108301 ggcaatcagc tggggtctgg agcaatttaa tcatccgttt gggcaactgg cgtttatcgc
   108361 gaccacgctg gttgggctgt acccgattgc tcgtcaggca ttacggctga tcaaatccgg
   108421 cagctacttc gccattgaaa ccttaatgag cgtagccgct attggtgcac tgtttattgg
   108481 cgcaacggct gaagctgcga tggtgttgct gctgtttttg attggtgaac gactggaagg
   108541 ctgggccgcc agccgcgcgc gtcagggcgt tagcgcgtta atggcgctga aaccagaaac
   108601 cgccacgcgc ctgcgtaacg gtgagcggga agaggtggcg attaacagcc tgcggcctgg
   108661 cgatgtgatt gaagtcgccg caggtgggcg tttgcctgcc gacggtaaac tgctctcacc
   108721 gtttgccagt tttgatgaaa gcgccctgac cggagaatcc attccggtgg agcgcgcgac
   108781 gggcgataaa gttcctgcag gagccaccag cgtagaccgt ctggtaacgc tggaagtgct
   108841 gtcagaaccg ggtgccagcg ccattgaccg gattctgaaa ctgatcgaag aagccgaaga
   108901 gcgccgcgcc cccattgagc ggtttatcga ccgtttcagc cgtatttata cgccagcgat
   108961 tatggccgtc gccctgctgg tgacgctggt gccaccgctg ctgtttgccg ccagctggca
   109021 ggagtggatt tataaagggc tgacgctgct gctgattggc tgcccgtgtg cgttagttat
   109081 ctccacgcct gcggcgatta cctccgggct ggcggcggca gcgcgtcgtg gggcgttgat
   109141 taaaggcgga gcggcgctgg aacagctggg tcgtgttact caggtggcgt ttgataaaac
   109201 cggtacgctg accgtcggta aaccgcgcgt taccgcgatt catccggcaa cgggtattag
   109261 tgaatctgaa ctgctgacac tggcggcggc ggtcgagcaa ggcgcgacgc atccactggc
   109321 gcaggccatc gtacgcgaag cacaggttgc tgaactcgcc attcccaccg ccgaatcaca
   109381 gcgggcgctg gtcgggtctg gcattgaagc gcaggttaac ggtgagcgcg tattgatttg
   109441 cgctgccggg aaacatcccg ctgatgcatt tgctggtttg attaatgaac tggaaagcgc
   109501 cgggcaaacg gttgtgctgg tagtacgtaa tgatgacgtg ctgggtatca ttgcattgca
   109561 ggataccctg cgcgccgacg cagcaactgc catcagtgaa ctgaacgcgc tgggggttaa
   109621 aggagtgatc ctcaccggcg ataatccacg cgcagcggcg gcaattgccg gggagctggg
   109681 gctggagttt aaagcgggcc tattgccgga agataaagtc aaagcggtga ccaagctgaa
   109741 tcaacatgcg cccctggcga tggtcggtga tggtattaac gacgcgccag cgatgaaagc
   109801 tgccgccatt gggattgcaa tgggcagtgg cacagacgtg gcgctggaaa ccgccgacgc
   109861 agcattaacc cataaccacc tgcgcggcct ggtgcaaatg attgaactgg cacgcgccac
   109921 tcacgccaat atccgccaga acatcactat tgcgctgggg ctgaaaggga tcttcctcgt
   109981 caccacgctg ctagggatga ccggactatg gctggcggtg ctggcagata ccggtgcgac
   110041 cgtgctggta acagcgaatg cgttaagatt gttgcgcagg agataaggca aaccgatcgc
   110101 aacattgagc gcgatcggtc ccctcgctcc tctggggaga gggttagggt gaggggaaaa
   110161 ggcggcatcg aagccaatct ttccccatca accgccttta cgaatcaaat aacgataagg
   110221 cagtccatcc gtctctttcg caaccagttc gtgttccata aaggtacaaa acccaggaat
   110281 atcgcgggtc gtcgccggat cgtcggcgat aatcagtaaa gtttcgccag gctgcatatt
   110341 gcgcacggtt ttgcgcacca tcatcaccgg ttccgggcag cgcaggccaa gcgcgtcgag
   110401 tgtgtggtca gggctggaaa agagatcggt cattttcttc tcatcactta aaaaaacggc
   110461 gctagtttac gccctgtgag tccgtaagca aaccaggtta acgattgcgt gaaaattaac
   110521 cattgcattg tcaacgtaaa gcagtatcat gcggcggctc gaaaaaaggg taagcacgtt
   110581 attatgttaa gataacagac gtgtcgtacg tattgggttc cctcacccca atggttaatc
   110641 aaaaaggtac aatatgaacg ttttctcgca aactcaacgc tataaggcgt tgttctggtt
   110701 atcgttattt catctgctgg tgatcacctc cagtaactat ctggtccagc ttccggtctc
   110761 catttttggt tttcatacca cctggggcgc gtttagtttt ccgtttatct ttctcgcgac
   110821 cgacctgacc gtgcgtattt ttggcgcacc gctggcccga cgcattatct tcgcggtaat
   110881 gatccctgcg ttattaatct cctatgtcat ctcgtcgctg ttctatatgg gttcatggca
   110941 gggattcggc gcactcgccc acttcaacct gtttgtcgcc cgtatcgcca ccgccagttt
   111001 catggcctac gcgctggggc aaatcctcga cgtgcacgtt tttaaccgcc tgcgtcaaag
   111061 tcgccgctgg tggctggcac cgacagcgtc cacactgttc ggtaacgtca gtgacacgct
   111121 ggcctttttc ttcattgcct tctggcgtag cccggatgcc tttatggctg aacactggat
   111181 ggaaatcgcg ctggtcgatt actgtttcaa agtgttaatc agtatcgttt tcttcctgcc
   111241 aatgtatggc gtattactca atatgctgtt gaaaagactg gcagataaat ccgaaatcaa
   111301 cgctttgcag gcgagttaaa ggttcgttat cagagttgtg ataagatgga tgaatgagcc
   111361 gttatggccg tttatcgaaa ggaagaagtc aatgcgcaat ctggttaaat atgtcggaat
   111421 tggcctgctg gttatggggc ttgcggcctg tgatgataaa gacactaacg ctacggcgca
   111481 gggttcggtc gcggaaagta acgctaccgg gaatcccgtc aacctgcttg atggcaagtt
   111541 aagtttctcg ctgccagcgg atatgaccga ccagagcggt aagctgggaa cgcaggccaa
   111601 taacatgcat gtctggtccg acgccaccgg gcagaaagca gtcatcgtca tcatgggcga
   111661 tgatccgaaa gaagatctgg cggtgctggc gaagcgtctg gaagatcagc aacgtagccg
   111721 cgatccgcag ctgcaagtgg taaccaataa agccattgag ctgaaaggtc acaaaatgca
   111781 gcagttagac agtattatct ccgcgaaagg ccagacggcg tactcttccg ttattctggg
   111841 taacgtgggt aatcaactgc tgaccatgca aattacgctg cccgctgacg atcagcaaaa
   111901 agcgcagacc accgcagaaa acatcattaa tacgctggtt cttcagtaag ttttaagatg
   111961 atgaggcggc ctcagggacg tgttccggag gccgtttttt taatcgccac gtcagtaata
   112021 acgcgattgc gaccagtccc gccgccgcca gataaatcac cggtacgccc gcccagctca
   112081 tcaccagccc agccagtggt ccagtcacgc caagcgataa atccataaat acggtgtaag
   112141 ttgccagcgc cgccccctga ttttgctgcg gaaccgcttt taccgccact acacccaatg
   112201 ccgggaacac cagcgaaaac ccggcccccg ccagtaagac gccgattttc gccatccacg
   112261 gcatagtcgc cacgccaacc agtagcaggc cgattatctc aacgctaaag caaatcatcg
   112321 ctacgtttaa gccaccgata cggttaatgc cgttagggaa taacaaacgc gtaccgacaa
   112381 acgcacagct aaacagcgtc agcgcgaaag ccgcaccgtc ccaaccttta gcgtcataaa
   112441 acagcgtgat aaaggtggcg atgacgccaa atccggcgga agccagtgcc agcgccatac
   112501 cgtacagcca gacgctccca agcaccgcgc gaaacggcag cggtttgcct ttactggctt
   112561 ttaccgtcgg acgcgggatc gccaacaaaa tggccaccag cgccacgccc ataatgatta
   112621 acgctaacgc ctgcaagccg ccccagtgat aaaacacgac gcctaacggc gcacccatcg
   112681 ccatcgcccc gtaagtgaca atgccgttcc acgaaatcac ccgcccgata tgcagcgagc
   112741 caaccacgcc aacgccccac agggtcgatc ccgttccggc aaaactttgc ccaatcccaa
   112801 ggatgacgcg ccccaggcaa agtaataaca ggctgatgac aggcagactg gcggttaatc
   112861 ctgccgtcag ataccccaga ccgctcaaaa agcagccgca taaaccgaag acgacaatct
   112921 ttttgggtcc cagcaaatcg gcataacgtc cggcatgagg gcggctcagc aaggtggcga
   112981 aatattgcag gctgataacc aatcctgccc agaaagcgct aaagcccatc acatcatgga
   113041 catagcccgg taatacagcg agcggcaacc cgatggtgag gtagctggcg aagttaaaca
   113101 tgacaatgga gacaatgcgc aaattcaggc gcaatccgtt tagcgcgggt tcggctacgg
   113161 gttcgggcat gaggatcacc acatttttac aacagtgttt catttttacc atgtgctgac
   113221 gtgaaaatca gcagtaagaa tcagaatatt gctggcgtga ctcccgctac acttaataca
   113281 aaaagtcaca aggaagcccc aatggaaacc cctcaacccg ataaaacggg catgcacatt
   113341 ctgctcaagc tggcctcgct ggtagtgatc ctcgcgggca ttcacgcagc ggcagatatc
   113401 attgtgcagc tgttactggc gctgtttttt gccatcgtcc tcaacccgct cgtcacctgg
   113461 tttattcgtc ggggagtaca acgccccgtt gccattacga ttgtagtggt ggtgatgctg
   113521 atcgcactaa ccgcgctggt cggcgtactg gcggcatcgt ttaacgaatt tatctctatg
   113581 ctgccgaagt ttaataagga gctgacgcgc aaacttttta aattgcagga gatgttgcct
   113641 tttcttaatt tgcatatgtc gccggagcga atgctgcagc ggatggactc ggaaaaagtg
   113701 gttaccttca ccacagcgct aatgaccggg ctttccgggg caatggcgag cgtgcttttg
   113761 ctggtgatga ccgtagtttt tatgctgttt gaagtgcgcc acgtccctta caaaatgcgt
   113821 tttgcgctga ataatccaca gattcacatc gcgggattac accgcgcact taaaggcgtt
   113881 tcgcactatc ttgcattgaa gacgctactc agtttatgga caggtgtcat cgtctggctg
   113941 gggctggagc tgatgggggt acagtttgcg ctgatgtggg cagtactggc gtttttgctt
   114001 aactacgtgc ccaatatcgg cgcggtaatt tccgccgtac cgccaatgat tcaggtgctg
   114061 ctgtttaatg gtatttacga atgtattctg gtcggcgcat tgtttttagt tgtccatatg
   114121 gtcatcggca atattttaga gccacggatg atgggccatc gcctggggat gtccaccatg
   114181 gtggtatttc tttcattgtt aatctgggga tggctgctcg gcccggtagg gatgctactt
   114241 tcggtgccat taaccagcgt gtgtaaaatc tggatggaaa cgacgaaagg tggcagcaaa
   114301 ctggcgattt tactggggcc gggcagaccg aaaagtcggt taccgggatg aggcgacaag
   114361 tgatacgata cgcactttca ttttccatta aacgttggcc ctgatatgta tcggatagtt
   114421 ctggggaaag tttcgacctt aagcgcagct ccactgccac cgggtttacg cgagcaagca
   114481 ccgcaaggtc cacgacgcga acgctggctg gcggggcgtg cattgctttc gcacacgctt
   114541 tccccgctac cggagatcat ctatggcgaa caaggcaaac ctgcattcgc gccggaaacg
   114601 ccgctatggt tcaacttaag ccatagcggt gacgatatcg ctctgctgtt gagtgatgaa
   114661 ggcgaagtcg gctgcgatat cgaagtgatt cgcccgcgcg ccaactggcg ctggctggcg
   114721 aacgccgtat tcagcctcgg ggaacacgct gagatggacg ccgtgcatcc tgatcagcaa
   114781 ctggaaatgt tctggcgcat ctggacgcgc aaagaagcca tcgttaaaca gcgtggcggc
   114841 agcgcctggc aaatcgtcag cgtagacagc acctatcact cctcgctgtc agtcagccat
   114901 tgtcagcttg aaaatttaag cctggcgatc tgcaccccca ccccctttac gctcaccgcc
   114961 gacagtgtgc aatggatcga ttcagttaac tgatccgccc acccgactgc ccatctattg
   115021 atccagaaca ggtaatcagt atgacgaata cttaaaatcg tcatacttat ttccgccatc
   115081 tattttaatc cattggggtt accatgctct ccacactccg ccgcactcta tttgcgctgc
   115141 tggcttgtgc gtcttttatc gtccatgccg ctgcaccaga tgaaatcacc accgcctggc
   115201 cggtgaatgt cgggccacta aacccgcacc tttacacgcc taaccagatg ttcgcccaga
   115261 gcatggttta tgaaccattg gtgaaatatc aggcagacgg ttcggtgatc ccgtggctgg
   115321 caaaaagctg gactcattca gaagatggta aaacctggac cttcaccctg cgtgatgacg
   115381 tgaaattctc caacggtgaa ccgttcgatg ccgaggcggc ggcagaaaac ttccgcgcag
   115441 tgctcgataa ccgtcaacgt cacgcctggc tggagctggc aaaccagatt gttgatgtta
   115501 aagcactcag taaaacagag ctgcaaatta ccctgaaaag cgcctactat cctttcctgc
   115561 aagaactggc cctgccccgt cctttccgtt ttatcgctcc ctcgcagttt aaaaaccatg
   115621 aaaccatgaa cggaattaaa gcgccgattg gcaccggacc gtggattttg caggaatcga
   115681 aactgaatca gtacgatgtc ttcgtccgta acgaaaacta ctggggcgaa aagccagcga
   115741 ttaaaaagat cacctttaac gtcatcccgg acccgactac ccgcgcggtg gcgtttgaaa
   115801 ctggcgatat cgacctgctg tacggaaacg aagggttatt accgctcgat accttcgccc
   115861 gctttagcca gaatccggct taccacaccc aactgtcaca gccgatcgaa accgtgatgc
   115921 tggcgctcaa taccgccaaa gcccccacca acgagctggc agtacgtgaa gctcttaatt
   115981 acgcggtaaa caaaaaatcg ctgattgata acgcgttata tggcacccag caggtcgccg
   116041 acaccctgtt tgccccttct gtgccttacg ctaatctcgg cctgaaaccg cgccagtacg
   116101 atccgcagaa agcgaaagag ttgctggaaa aagccggttg gacgctgcct gcgggcaaag
   116161 acatccgcga gaaaaatggt cagccgctgc gcattgaact gtcgttcatc ggcaccgatg
   116221 cgttaagcaa atcgatggcg gaaatcattc aggctgatat gcgccagatt ggcgcagatg
   116281 tctcgctgat tggcgaagaa gagagcagta tctatgctcg tcagcgcgac ggtcgttttg
   116341 gcatgatttt ccaccgcacc tggggcgcgc catacgatcc acacgccttc ctgagttcaa
   116401 tgcgcgtacc gtcgcacgct gacttccagg cacagcaagg attagccgac aagccgctga
   116461 ttgataaaga gatcggcgaa gtgctggcga cccatgacga aacgcaacgt caggcgctgt
   116521 atcgcgacat tctgacccgt ctgcatgacg aggcggttta tctgcctatc agctacatct
   116581 cgatgatggt ggtatcaaaa ccggagctgg gtaacatccc ctacgcgccg atcgccaccg
   116641 aaattccgtt cgaacagatt aaaccggtga aaccttaatg ttgcgttacg tattacgccg
   116701 ctttctgctg ctgatcccga tggtgctcgc cgcctcggtg atcatttttc tgatgctgcg
   116761 cctcggtacc ggcgacccgg cgctcgatta tttgcgtctg tctaacctgc cgccgacgcc
   116821 ggagatgctg gcctctaccc gcaccatgct gggactggat cagccgctgt acgtccagta
   116881 cggcacctgg ttgtggaagg cgctgcatct tgactttggt atctcattcg ccagccaacg
   116941 cccggtactg gacgatatgc tgaacttcct gcccgccacg ctggaacttg caggtgcggc
   117001 gctggtatta attctgctca cttccgtacc gctcggtatc tgggcggcgc gccaccgcga
   117061 ccgtctgccg gatttcgccg tacgtttcat cgcgtttctt ggcgtgtcga tgcctaactt
   117121 ctggctggcg tttttactgg tgatggcgtt ttcggtgtat ctgcaatggc tacccgcgat
   117181 gggttacggc ggctggcagc acatcatttt gcctgcggtt tccattgcct ttatgtcgct
   117241 ggcgattaac gcgcgtttac tgcgcgccag tatgctggac gtcgccggtc agcgtcacgt
   117301 cacctgggcg cgtctgcgcg gcctgaacga caaacagacc gaacgtcgcc acatcctgcg
   117361 caatgcctcg ctgccgatga tcaccgccgt ggggatgcac atcggcgaac tgatcggcgg
   117421 gacgatgatt atcgaaaaca tctttgcctg gccgggcgtc gggcgctatg cggtgtcggc
   117481 gatttttaac cgtgactatc cggtgatcca gtgctttacg ctgatgatgg tggtggtttt
   117541 tgtggtctgt aatttgattg tcgatttgct caacgccgcg ctggacccgc gcattcgtcg
   117601 tcatgaagga gcgcacgcgt gaactttttc ctctcttccc gctggtcggt acgcctggcg
   117661 ctgatcatta tcgccctgct ggcgctgatt gcgcttacca gccagtggtg gctgccgtat
   117721 gaccctcagg ctattgatct gccgtcgcgc ctgctttcgc cggatgcgca gcactggctg
   117781 ggcaccgatc acttagggcg tgatattttc tcgcggctga tggcggcgac ccgcgtgtcg
   117841 ctcggttcgg taatggcctg cctgctgctg gtgctgacat tagggctggt gattggcggc
   117901 agcgccggac tgattggcgg acgcgttgat caggccacca tgcgcgtcgc cgatatgttt
   117961 atgaccttcc cgacctcgat tctgtcgttc tttatggttg gcgtgctcgg caccgggctg
   118021 accaacgtaa ttatcgccat cgccctgtcg cactgggcgt ggtatgcacg catggtgcgc
   118081 agcctggtga tttcactacg ccaacgcgag tttgtgctgg cgtcacggct ttccggtgcg
   118141 ggccatgtgc gggtgtttgt cgatcatctg gcaggcgcgg tgatcccttc gctgctggtg
   118201 ctggcaacgc tggatatcgg ccatatgatg ctgcacgtcg cggggatgtc tttccttggc
   118261 ctcggtgtga ccgcgccgac cgccgaatgg ggcgtgatga ttaacgacgc gcgccagtat
   118321 atctggaccc agccgctgca aatgttctgg ccggggctgg ctctgtttat cagcgtgatg
   118381 gcctttaacc tggtgggtga cgcactgcgc gatcatctgg atcctcatct ggtgacggag
   118441 cacgcacact aatgccgcaa cagattgaac tacgtaatat cgcgctacag gccgcgcagc
   118501 cgctggtaca cggtgtatcg ttaaccctgc aacgcgggcg cgtgctggcg ttagtcggcg
   118561 gtagcggcag cgggaaatca ttaacctgcg ccgcgacgct gggcattttg cccgctggcg
   118621 ttcgccagac ggcgggggaa attttagccg atggcaaacc ggtttcgcct tgcgccctgc
   118681 gcggcatcaa aattgccacc atcatgcaga acccgcgcag cgcctttaat ccactgcaca
   118741 ccatgcacac ccacgcgcgg gaaacctgcc tggcgttagg gaaacccgcc gatgacgcta
   118801 cgcttaccgc tgccatagaa gcggtggggc tggaaaacgc cgcgcgcgtg ctgaagctgt
   118861 acccgttcga gatgagcggc ggcatgttgc agcgcatgat gattgcgatg gcggtgctgt
   118921 gtgaatcacc gtttatcatc gccgatgaac cgaccaccga cctcgacgtg gtagcacagg
   118981 cgcgcatcct cgatctgctg gaaagcatta tgcaaaaaca agcgccggga atgctgctgg
   119041 tcacccatga tatgggcgtt gtggcgcgtc tggcggatga cgtggcggtg atgtcacaag
   119101 gtaaaattgt cgaacagggc gatgtagaaa cgctgtttaa cgcccccaaa catacggtga
   119161 cgcgcagcct ggtttccgct catctcgccc tctacggtat ggagctggca tcatgacctt
   119221 acttaacgtc tgcggccttt cccatcacta tgcccacggt ggatttagcg gaaaacatca
   119281 acatcaggcg gtgctgaata acgtttccct gaccctgaaa agcggcgaaa ccgtcgccct
   119341 gctgggacgc agcggctgcg gaaaaagcac cctcgcgcgg ctgctggtag gcttagagtc
   119401 tccctcacaa gggaatatta gctggcgcgg cgaaccgctg gcgaaactca atcgcgccca
   119461 gcgtaaagcg ttccgccgcg atattcagat ggtatttcag gactccatca gcgccgtgaa
   119521 tccgcgcaaa accgtgcgcg agatcctgcg tgaaccgatg cgccacctgc tatcactgaa
   119581 aaaatccgaa caactggcgc gcgccagcga aatgctgaag gcggtcgatc tcgatgacag
   119641 cgttctcgac aaacgcccac cgcagttaag cggcggccag ctccagcgcg tctgcctggc
   119701 tcgcgcgctg gcggtcgaac cgaaactact gattctggat gaagccgttt ctaaccttga
   119761 tctcgtgtta caggcgggcg tcattcgcct gctgaaaaag ctacaacaac agtttggcac
   119821 cgcctgcctg ttcatcaccc acgacttacg cctggtggaa cgcttctgtc agcgggtgat
   119881 ggtgatggac aacggacaaa tcgtcgaaac ccaggtggtg ggagataaat taaccttttc
   119941 ctctgacgcc ggacgtgtgc tacaaaacgc ggtattaccc gcattccccg tgcgccgtcg
   120001 caccacagaa aaggtttaac gcaaatgcaa cgagtcacca tcacgcttga tgacgattta
   120061 ctggagacgc tggacagcct gagccagcgt cgtggttata acaaccgttc cgaagctatc
   120121 cgcgatattc tgcgtagcgc cctggcccag gaggccactc agcagcacgg cacgcaaggt
   120181 ttcgcggtgc tctcctatgt gtatgaacac gaaaaacgcg acttagccag ccgcattgtc
   120241 tccacccagc accatcacca cgatctctcc gtcgccacgc tgcatgtgca tatcaatcac
   120301 gacgactgtc tggaaatcgc cgtgctgaaa ggtgatatgg gcgacgtgca gcattttgcc
   120361 gatgacgtta tcgcccagcg cggcgtgcgg cacgggcatt tgcagtgctt gccgaaggaa
   120421 gattgagtta tttctgaaac gaggaaagaa aagatattaa ctgaacttaa tttataaaat
   120481 taagttcagt tgcatagata gatcctgtaa aggcagatat tctgcgtaat gtacgccgct
   120541 aaaatttata aatgattcaa acgctcattc agtgtttcaa taatataggt attaacagag
   120601 acttgttgct gcgcagccgc attattaagg cgttcactca gcgattctgg atagcgtaac
   120661 gtaaaggtct taatcttttc tgtacgcgca tacggctcaa tacccgcagc tttacaatcc
   120721 tcaagatact ctcgcaggga aagttcccct tctttttgca gcccctgaat gctgtcagag
   120781 acaaaatcac aataacctga caagcccagg aatttgccac gaaatgcatt cagttctggc
   120841 acgtaagtga taacagcagg ttgcccggca atctccatag agtttggcgt cttcagtttg
   120901 atcatggttt tacccctata cttagtaacc agtcacgcac gctttctaca gcgcctttat
   120961 cggtatctgg cgacggatga ggccgatgga aacacgcaac gctcatattt agtatgaact
   121021 tacagcgcga ccccctacct tccttaattt ctccgcctaa cgctttaacc agagattcta
   121081 tatcagtcca tttgattcct tgaggtaaag gtgatttaaa tagctgatcc agtgtgtttt
   121141 tttgtttctt tcgtaaagcc tgtacctgtg tttacatgac attcccttat gacttcactt
   121201 tatgacatca cagtatgaag tcaagtttcc cttccttacg ccattgtccc aatcgtcttc
   121261 ctgaatcgca gcagcgcgat ggtgaaaaac gcgccgccaa ttgccatcag cgtcaggaac
   121321 tgcggccaga cgatttcgaa tccggcaccc cggtagagaa tggcctgcgc gaggctaaca
   121381 aagtgtgtcg tcggcatggt cagcataatg tcctgcacca tctgcggcat actttcgcgc
   121441 ggcgtggatc caccggaaag catttgcagc ggcagcagca ccagaatcac cagcagcccc
   121501 agttgcggca ttgaacgcgc tatcgtcccc ataaaaatgc cgattgacgt ggtggcaaac
   121561 agactgagcg ccacgcccag cataaacagc gggatcgagc cttcaatcgg tacacccagc
   121621 acgcctttca ccatcagcac cagtgataat cccgacacca ccagcaccac cagccccatc
   121681 gaccagacct ttgccatcat gatctcaaac ggcgttatcg gcatcaccaa caagtgttcc
   121741 accgtgccgt gttcacgctc gcggatcagc gccgatccgg tcaatacaat cgccagcatg
   121801 gtaatgttgt tgatgattgc catcaccccg ccaaaccacg cgggatcaag gttcgggtta
   121861 aagcgcatcc gggtttccag cgataccaac ggttcgctgt tatcacggta gcgcgcgaca
   121921 aagctgttca cttcaccgtt gataatattc tggatatacc cattgccggt aaatgcctgg
   121981 ctcatgcgcg tggcatcgac gttcacctga atatccggct ggcgtccggc gaggacatca
   122041 cgctgaaaat taggcggaat atttatcgca aaggtataac gcccggcgtc cagtccggca
   122101 tccatctcat cggcggtgat catctccggt ggcaaaaacc acgggcgata gaagctgtta
   122161 acgatccggt tcgataactg cgattgatcc atatcggcaa tggcgatcgg cgcgaggttc
   122221 aacgatcctg gcgtaacggt cgctgacgaa tacaccgaca ccgtaaacga gaagacaatc
   122281 agcgtcagca tcgctttatc accgagcaga ctgcgcaact ctttgatacc cagattaaaa
   122341 atattgcgta aatggcgcat catccctcct gttttttcag cagcaggata cttaagccca
   122401 tcaccagcgg gatggctatc agcaacggga taaaaagttg ccacaaatca gtcagatcca
   122461 gcgctttcga gaacgttccg cgggcgatag tcagaaaatg actggtcggg taaacctcgc
   122521 cgatccaacg tccaggccct tccagcgaag ctaccggatc gatcatcccg gaaaactgtg
   122581 tcgccgggat caacgtgata atcgccgttc cgaaaatggc ggcaatctgg cttttcataa
   122641 aggtggagat cagcagcccc attccggtgg caatgatgat atacagcagc gccgccaggg
   122701 tgagcgtcag gaaactgcct ttatgcggta cgccaaacac aaacaccgac aggccgcaga
   122761 gcaggaaaaa gttcagcatc cccagcgcga tgtatggcaa ctgtttacca agcaaaaatt
   122821 cactacgcgt ggtgggggtc acgtaaaggt tgataatcga cccaagctct ttttcccgca
   122881 ccacgctaag ggcgcttagc attgacggga tcatcatcag cagaagcggg atcaccgccg
   122941 gaacaatcgc tggcaggctt tttacgtccg ggttatagcg atagcgcgtc tcaatattca
   123001 tcagcccgct ttggctggcg ggtgtggatt gtcggctcgc cacatcctgt agccagctct
   123061 ggtgcattgc ctgcacgtaa ccttttaccg tttcggcacg gctcggcatc gctccgtcga
   123121 tccagacgcc gagttccaca ggcgtaccac gcgcgatatc gcgcccgaaa ttgggaggga
   123181 tctcaattgc caccgttata tcgcccgcac gcatccgacg atcaagctcg tcataactgg
   123241 tgagcggcgg ctgttcgata aagtaacggg aaccggagag attgagcgtc cacgcctggc
   123301 tactgacggt ctggtcgcgg tcgagcaccg caaagcgcag gttttccaca tccatactga
   123361 tgccgtaacc cattatcagc atcaggatca ctgttcccat cagcgccagc gtcgaacgta
   123421 ccggatcgcg tcgcagttcc agtgcttcgc ggcggctgta gctaaacaga cggcgcaggc
   123481 taaatccctg acgcggcgcg tgggtggtgt cgtgtatcac gggcggcgct tcggcttcgt
   123541 tgctctgtcc tgccgcttcc tgcaaatagg cgataaatgc ctcttccaga ctggcggctc
   123601 cgcgtttctc aaccagttcc tgcggtgtac cgctggcgag cacttttccg gcgtgcatca
   123661 gtgagatgcg gtcgcaacgt tccgcttcgt tcataaagtg ggtggagata aaaatggtta
   123721 ctttgtcctg gcgcgagaga tcgaccatca actgccagaa catatccctc gccaccggat
   123781 cgacgccaga agtaggttca tcgaggatta acatctccgg gcgatgaatc accgccaccg
   123841 ccagcgaaag ccgctggcga atgccgagcg gcaatgactc cggcagaaca tcttcaacgt
   123901 cgttgagctt aaaacgctcg ctcatttcag ccactcgcgc gggaatttcc gcttccggga
   123961 tgtgaaacaa acgggcatgt aactcaaggt tttgccgcac ggtgagttcg ttatagagcg
   124021 aaaacgcctg cgacatatag cccacccgac ggcgggtatc gatatctttt ggatcaaccg
   124081 gttgcccaaa cagccacgcc tcaccttcgc tggcgggcag cagtccggtg agcattttca
   124141 tggtggtgga tttaccgcag ccgttcgatc caagaaaacc aaaaatctcc ccgcgtggaa
   124201 tgcggaaatt gacgtgatca acggcaacga aggaaccaaa acgcatggtc agatcgcgcg
   124261 cttcgatggc aatctctgca ttttcaggtt gatacggtgg gatcactacc gcctgatgcg
   124321 cctggcgttg cgcttgcggt aacagattta taaacgcctc ttccagcgtg gcgctttgcg
   124381 tttgctgccg cagctcttcg gcgctgccag ttgccagcac ttctccggca ttcatcgcta
   124441 ccagccagtc gaagcgttcg gcctcttcca tataggcggt ggcgaccagc acgctcatat
   124501 tgctctgccg ctggcgaata ctgtcgatca gatcccagaa ctgggcgcgg gagagcgggt
   124561 caacccccgt tgttggctca tcaaggatca acagttccgg gtcgtggatt aacgcgcagc
   124621 acagccccag tttttgcttc atcccgccgg agagtttacc tgccggacga tcgcgaaacg
   124681 gtgctaaccc ggtgctggtc agcagctcat taattcggac ttcccgctcc gctttatcgt
   124741 gaccgaacaa gcgagcgaaa aaatcgacat tttcatacac cgataaggtg tggtagagat
   124801 ttttgcccag cccctgcggc atccaggcga tgcgcgggca gacgtcgcgg cgatgcttcg
   124861 ggtcgcgcat atcgccgccc agcaccatca cattgccctg ttcaatgacg cgggcaccgg
   124921 aaatcaacga caacaagctc gacttcccga cgccgtccgg gccaatcagc ccgaccatgc
   124981 aacgggccgg aatatcgagt gtgatattgt tcagcgcaac ggtttttcca taatgctggc
   125041 tcacgcccgc cagttgcgcg acaggcggga cgggaaccag ttccagatgc gtcattgcgg
   125101 caacctcacc acgaggtcgt caggccacgg aagttcttca ttcacccgca cccacgctac
   125161 gcccggtaaa ccggttttga catattccag atgctgctgg agtaattccg gtgggatacg
   125221 cgctttgacg cggaacatca gtttcagccg ttcatcgctg gtttcgacgg tttttggcgt
   125281 gaactgggcg acactggcga caaaactgat ggttgcagga atacgcagat ctggcgcggc
   125341 atcgaggatc agccgggctt caccgcccag tttcagcgtg cccgcctgtt cggttggcag
   125401 gaagaaagtc atatagacgt cgctgagatc gaccatattc agcacccgac cgcctgccgc
   125461 cagcacttcg cctggctcgg caacccgata ctgcacgcgt ccgtcacgcg gggctttcag
   125521 ttcgctgtca tcgatatctg cggcaatgcg ccgttcagtg gcttgtgcgg cttcgacgcg
   125581 agtttgcgcc tgaatgatat tggtgcgtgc cgcttctata gcggctttag aagccgaaac
   125641 ctgagctttt gccgattcca gcgcagctcg ggcgctctca gcggcggcgc ggtcgtcatc
   125701 caactgttgc gcagaaatag ccccacgatg ggcgagcgag cgggaacggg tatgacgttt
   125761 ggctacggag tccagttcag cttgccgttg attaaccagc gactgagcgg cgcgagtttc
   125821 actttggcgt tgctccagca aagcctgcgc ggcagcaacg gcgctttgtg cttctttgat
   125881 ttgcgcgatg gcttccagtc gctgttcctg caacacgcga gtatccatct tcgccagcac
   125941 ttcaccttct cgaacaaact ggccttcttt caccagaatg gtgtcaatac gcccggcaat
   126001 tttgctggcg atatccactt ccgtcgcttc aatgcgccca ttgctgacag caaagccttc
   126061 cggcacacct gccgggcgca acagccacca cgccacgata gccgccaccg ccagtaaccc
   126121 gacaacccac cacgccagat ggcgcttact cttatccata atcgacccgc cataatccct
   126181 gtaagcaaac gacgcgtgtt acaactgccg ccgaacgacg ttcagcggat tcttccatcc
   126241 gggataagaa ggaaacggca aatacgacca gcgcgcagag cattgagcgc caacggggaa
   126301 ggcttagtga aaacgggtag taatgttgtc atcatcagca ttcctggccg taaatgaaac
   126361 acgcatactc ataagtgtga tctataaaac tggctgacaa aacggtatcc atcctgttgg
   126421 atagcgtttc acgcctgttc agttgcagga acagagcatt cctttttacg ttttatcaac
   126481 agtctcaacc agatagtaac attaatattc atgttgtcta tggttcagag ctgtaacatt
   126541 gtgcgccttc taatcaaagg cataagtcca tttctgtttt tttcatgctg gtaagacttc
   126601 cagggagtgg ttatctcagg ccctgcacca gggttaattt ggcaattatg catgctgaac
   126661 attcttccag catgtttact gtcacgggcg ttaagaccta aaactgtccc cgtttcgcct
   126721 atgcgttctc ttccaccatt tccgttatgt gcaacaactt gatttatacc taacatttgt
   126781 gaacatgcta atagtgcttc aagtttaggt ctgtaatctg tcgaaacaga aaaataacga
   126841 ttaaaggatc gacaagagat agcccggcta gttttaaact ttttgccatt aattatagca
   126901 tgatgctttt tattgataat tgccgctaat tcgaccggat cgagagcatt ggtcatttca
   126961 tcaatagaga ctgttatcgg tcctataatg taacgttttc gctgctcatc aaaagttatc
   127021 ccatgatgat ttgccattat tttgtatttg gggtcataga aacatacagg ataatatttt
   127081 atcccagcgt aggtatcctc atcaagtggt ttcatacgcg tgaagttctg catatagtta
   127141 ccattgcaat tcgtttcatg atttccagct aatacatgaa cattttcatt gatactcatt
   127201 atgcgtttta acaaatcgat gataaattga tcgccacaga tattactaaa tcgatcgccg
   127261 gtttggttgc caagaaacgt acatggcgtc acagcgtctt tattaataac taaataaggc
   127321 tgaagacttt ctaaaactat atctcttctg ttcagaatac tgattattgt attaacatta
   127381 gtgtcaagtt gctcattaaa gttctccctt agcaatacgt tgagaacttc atcttctata
   127441 ttaagcaact ggctaaggaa attttctcct tcagccatga ttctcaaatg tcccgttgca
   127501 aaaagcgcgt acaaaactgc accgactgag ccatcggtat cgccaaaata ggttggtctg
   127561 gtacttgtgg gatcaacagg tgaacaattg gagaaatagg ttctgtcagc gaactggggg
   127621 tttatcattg tttcgaattt aatatcagat acattgaatt gcgatatttt ccagtcccag
   127681 acgggtttgt acgactcagt aactgcaaca tcattagcat catcatttac tgtcatatta
   127741 ttaagtaaat aaacaatgct gcaccaatta atatcggtat tatcaagata gcagccgtaa
   127801 tttttactat taattgtcac aataaactga taattattat ccctacgtag aatatgagcg
   127861 cgcatgtccg atagcatact atatttttct gaatgattcg tttgtatggt aaatgatatt
   127921 ttgtcatctc gaatagattg ttggaaacgt tctatttgcc aatcatcaat tttttccaca
   127981 acttcaccga gcattgtcct ggcaatactt gatatatcat cacctatacc atttgcaata
   128041 tacccaaaca gttctttagt gaagttagta acatgctctt gcaccatatc atttgttgcg
   128101 attgtcgttg tactgtcgtt agtgcctgcc acagttccaa ttttcatctt ttacctcata
   128161 taattcaccg cgaggaatgc atagttaaca tttaatctta tgacatacac ctcatgttcg
   128221 atggaaaaat aattatattt tgcacaaatc attcagcctt aaagcataat atgaaatttc
   128281 ttatctcata gcagagaaag tccctaaaga ttgtagaggc gtcatcagaa catgcctcta
   128341 caatcgatta agaaaaccta tgaaatactg gcaggattaa aaagatatac cacagcactc
   128401 atggagtttt aatgtggttg aaaacaacta agccctggga cacctttttt atcaccacgt
   128461 tcgttaggag ctatcttcca ggaatttagc cccatggtat aattatcaat tttttgatgc
   128521 tgatctaccc ctaatccata atggccatat ttctgtttag tttttttgag cgtctgtcca
   128581 catttgaaga tatcttcagg ttttggtctg aaagcagtag tgttattaaa ataattgtca
   128641 tatacaggtg tggatgctat agcattgctt ctaattagat ggataaaacg atcatccatg
   128701 tgttctttat gttttttatt aaaaatattt gctaattcct caggatcagt aggatttttc
   128761 atctgattaa atggaacctg caaagcaccg agacaataac attttttttc ttcatctctt
   128821 attattccgt gatgactggt taaaacttgc ctttctgagt cataattaca aacatcgagc
   128881 gttttaatta agttatacgt atcaccggca gagagtttat gatttgctag tcgtgcagta
   128941 tagttaccat taaaattgat ttcatgattt ccagccagaa cgacgacatt tttattaata
   129001 cggctatctt tattaccctc catatttcgc atagaattta gcaaagtaag gatatattta
   129061 tcaccaaaaa tagtactaaa tctatcgcct gtatgatccc caaggaagat acaaggggtt
   129121 atcgcatcgt cctttaaaaa aacatgttga tgcaactcat tcaagatcat gtctctcgta
   129181 tttaataaat cgtagagcac tttattattt ttatgtttgt atgcagatga agccatctct
   129241 tcgtgtttta ataattcaca taataaactc caaccctttt ccctgatgcc tatatgccct
   129301 gaaacgagca atgcataaag tacagcgcct actcttccat ctgtatcgcc aaaataagcc
   129361 gtgttggcat tttgtgggtt aatcacacca cactgtttat agaagtctat ttttgaaaac
   129421 acaggtttaa catagctatc aaacttttcc ttagtaaacg tctctttaga gacatcccaa
   129481 cgccagtcag gtttgaaatt accactaaga agagtaaaga aatttctcgc tatgatagaa
   129541 agcccatcaa ctttataaat tatatctgat ttatctccag aggaattaat aaaataataa
   129601 ccgcccttac actcaacagt aacggtaatg ggagacgata attttaacac atggttattg
   129661 tctggtataa ttgtaaagat aaacgtatca gtag
//
LOCUS       DAFNBJ010000002        87709 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000002 DAFNBJ010000000
VERSION     DAFNBJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.002"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(123..1598)
                     /gene="ampG"
                     /locus_tag="L2X49_000119"
     CDS             complement(123..1598)
                     /gene="ampG"
                     /locus_tag="L2X49_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBN4795273.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1642..2220)
                     /locus_tag="L2X49_000120"
     CDS             complement(1642..2220)
                     /locus_tag="L2X49_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBN4795274.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2525..2842
                     /gene="bolA"
                     /locus_tag="L2X49_000121"
     CDS             2525..2842
                     /gene="bolA"
                     /locus_tag="L2X49_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBN4795275.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3186..4484
                     /gene="tig"
                     /locus_tag="L2X49_000122"
     CDS             3186..4484
                     /gene="tig"
                     /locus_tag="L2X49_000122"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBN4795276.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4730..5353
                     /gene="clpP"
                     /locus_tag="L2X49_000123"
     CDS             4730..5353
                     /gene="clpP"
                     /locus_tag="L2X49_000123"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBN4795277.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5479..6753
                     /gene="clpX"
                     /locus_tag="L2X49_000124"
     CDS             5479..6753
                     /gene="clpX"
                     /locus_tag="L2X49_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBN4795278.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATIKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6941..9295
                     /gene="lon"
                     /locus_tag="L2X49_000125"
     CDS             6941..9295
                     /gene="lon"
                     /locus_tag="L2X49_000125"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBN4795279.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9504..9776
                     /gene="hupB"
                     /locus_tag="L2X49_000126"
     CDS             9504..9776
                     /gene="hupB"
                     /locus_tag="L2X49_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBN4795280.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            9968..11839
                     /gene="ppiD"
                     /locus_tag="L2X49_000127"
     CDS             9968..11839
                     /gene="ppiD"
                     /locus_tag="L2X49_000127"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBN4795281.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQTAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            11990..12361
                     /locus_tag="L2X49_000128"
     CDS             11990..12361
                     /locus_tag="L2X49_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBN4795282.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12455..12853
                     /gene="fadM"
                     /locus_tag="L2X49_000129"
     CDS             12455..12853
                     /gene="fadM"
                     /locus_tag="L2X49_000129"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBN4795283.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12905..13600)
                     /gene="queC"
                     /locus_tag="L2X49_000130"
     CDS             complement(12905..13600)
                     /gene="queC"
                     /locus_tag="L2X49_000130"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBN4795284.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(13665..15365)
                     /locus_tag="L2X49_000131"
     CDS             complement(13665..15365)
                     /locus_tag="L2X49_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HBN4795285.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPQFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15465..16283
                     /gene="cof"
                     /locus_tag="L2X49_000132"
     CDS             15465..16283
                     /gene="cof"
                     /locus_tag="L2X49_000132"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBN4795286.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16436..16894
                     /gene="decR"
                     /locus_tag="L2X49_000133"
     CDS             16436..16894
                     /gene="decR"
                     /locus_tag="L2X49_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBN4795287.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16924..18696
                     /locus_tag="L2X49_000134"
     CDS             16924..18696
                     /locus_tag="L2X49_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBN4795288.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            18689..20470
                     /locus_tag="L2X49_000135"
     CDS             18689..20470
                     /locus_tag="L2X49_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBN4795289.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20651..20989
                     /gene="glnK"
                     /locus_tag="L2X49_000136"
     CDS             20651..20989
                     /gene="glnK"
                     /locus_tag="L2X49_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBN4795290.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21019..22305
                     /gene="amtB"
                     /locus_tag="L2X49_000137"
     CDS             21019..22305
                     /gene="amtB"
                     /locus_tag="L2X49_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBN4795291.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22354..23214)
                     /gene="tesB"
                     /locus_tag="L2X49_000138"
     CDS             complement(22354..23214)
                     /gene="tesB"
                     /locus_tag="L2X49_000138"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBN4795292.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23432..24004
                     /locus_tag="L2X49_000139"
     CDS             23432..24004
                     /locus_tag="L2X49_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBN4795293.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24035..24346)
                     /locus_tag="L2X49_000140"
     CDS             complement(24035..24346)
                     /locus_tag="L2X49_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBN4795294.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24509..24605
                     /gene="ffs"
                     /locus_tag="L2X49_000141"
     ncRNA           24509..24605
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="L2X49_000141"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24725..25078
                     /locus_tag="L2X49_000142"
     CDS             24725..25078
                     /locus_tag="L2X49_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBN4795295.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25120..26670)
                     /gene="pdeB"
                     /locus_tag="L2X49_000143"
     CDS             complement(25120..26670)
                     /gene="pdeB"
                     /locus_tag="L2X49_000143"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBN4795296.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITGSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26834..27304)
                     /locus_tag="L2X49_000144"
     CDS             complement(26834..27304)
                     /locus_tag="L2X49_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBN4795297.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27420..27971)
                     /gene="maa"
                     /locus_tag="L2X49_000145"
     CDS             complement(27420..27971)
                     /gene="maa"
                     /locus_tag="L2X49_000145"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBN4795298.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPNNVVVGGNPARIIKKL"
     gene            complement(28143..28361)
                     /gene="hha"
                     /locus_tag="L2X49_000146"
     CDS             complement(28143..28361)
                     /gene="hha"
                     /locus_tag="L2X49_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBN4795299.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28387..28761)
                     /gene="tomB"
                     /locus_tag="L2X49_000147"
     CDS             complement(28387..28761)
                     /gene="tomB"
                     /locus_tag="L2X49_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBN4795300.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(29307..32456)
                     /locus_tag="L2X49_000148"
     CDS             complement(29307..32456)
                     /locus_tag="L2X49_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBN4795301.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(32479..33672)
                     /gene="acrA"
                     /locus_tag="L2X49_000149"
     CDS             complement(32479..33672)
                     /gene="acrA"
                     /locus_tag="L2X49_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBN4795302.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            33814..34461
                     /gene="acrR"
                     /locus_tag="L2X49_000150"
     CDS             33814..34461
                     /gene="acrR"
                     /locus_tag="L2X49_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBN4795303.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMENIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            34589..37951
                     /gene="mscK"
                     /locus_tag="L2X49_000151"
     CDS             34589..37951
                     /gene="mscK"
                     /locus_tag="L2X49_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBN4795304.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPALG"
     gene            complement(38163..38324)
                     /gene="rsmS"
                     /locus_tag="L2X49_000152"
     CDS             complement(38163..38324)
                     /gene="rsmS"
                     /locus_tag="L2X49_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBN4795305.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(38338..38865)
                     /gene="priC"
                     /locus_tag="L2X49_000153"
     CDS             complement(38338..38865)
                     /gene="priC"
                     /locus_tag="L2X49_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBN4795306.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38935..39312
                     /locus_tag="L2X49_000154"
     CDS             38935..39312
                     /locus_tag="L2X49_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBN4795307.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39465..40016
                     /gene="apt"
                     /locus_tag="L2X49_000155"
     CDS             39465..40016
                     /gene="apt"
                     /locus_tag="L2X49_000155"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBN4795308.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            40145..42076
                     /gene="dnaX"
                     /locus_tag="L2X49_000156"
     CDS             40145..42076
                     /gene="dnaX"
                     /locus_tag="L2X49_000156"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBN4795309.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    41409..41473
                     /gene="dnaX"
                     /locus_tag="L2X49_000156"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            42129..42458
                     /locus_tag="L2X49_000157"
     CDS             42129..42458
                     /locus_tag="L2X49_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBN4795310.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            42458..43063
                     /gene="recR"
                     /locus_tag="L2X49_000158"
     CDS             42458..43063
                     /gene="recR"
                     /locus_tag="L2X49_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBN4795311.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            43173..45047
                     /gene="htpG"
                     /locus_tag="L2X49_000159"
     CDS             43173..45047
                     /gene="htpG"
                     /locus_tag="L2X49_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBN4795312.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSGITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            45228..45872
                     /gene="adk"
                     /locus_tag="L2X49_000160"
     CDS             45228..45872
                     /gene="adk"
                     /locus_tag="L2X49_000160"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBN4795313.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            46108..47070
                     /gene="hemH"
                     /locus_tag="L2X49_000161"
     CDS             46108..47070
                     /gene="hemH"
                     /locus_tag="L2X49_000161"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBN4795314.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(47067..48026)
                     /gene="aes"
                     /locus_tag="L2X49_000162"
     CDS             complement(47067..48026)
                     /gene="aes"
                     /locus_tag="L2X49_000162"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBN4795315.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            48178..49482
                     /gene="gsk"
                     /locus_tag="L2X49_000163"
     CDS             48178..49482
                     /gene="gsk"
                     /locus_tag="L2X49_000163"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBN4795316.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(49615..51291)
                     /locus_tag="L2X49_000164"
     CDS             complement(49615..51291)
                     /locus_tag="L2X49_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBN4795317.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(51529..52749)
                     /gene="fsr"
                     /locus_tag="L2X49_000165"
     CDS             complement(51529..52749)
                     /gene="fsr"
                     /locus_tag="L2X49_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBN4795318.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            52967..54619
                     /gene="ushA"
                     /locus_tag="L2X49_000166"
     CDS             52967..54619
                     /gene="ushA"
                     /locus_tag="L2X49_000166"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBN4795319.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(54656..55135)
                     /gene="ybaK"
                     /locus_tag="L2X49_000167"
     CDS             complement(54656..55135)
                     /gene="ybaK"
                     /locus_tag="L2X49_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBN4795320.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(55339..56133)
                     /locus_tag="L2X49_000168"
     CDS             complement(55339..56133)
                     /locus_tag="L2X49_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBN4795321.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            56271..56612
                     /locus_tag="L2X49_000169"
     CDS             56271..56612
                     /locus_tag="L2X49_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBN4795322.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDFWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(56726..59230)
                     /gene="copA"
                     /locus_tag="L2X49_000170"
     CDS             complement(56726..59230)
                     /gene="copA"
                     /locus_tag="L2X49_000170"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBN4795323.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            59492..60424
                     /gene="glsA"
                     /locus_tag="L2X49_000171"
     CDS             59492..60424
                     /gene="glsA"
                     /locus_tag="L2X49_000171"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBN4795324.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            60427..61719
                     /locus_tag="L2X49_000172"
     CDS             60427..61719
                     /locus_tag="L2X49_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBN4795325.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            61844..62251
                     /gene="cueR"
                     /locus_tag="L2X49_000173"
     CDS             61844..62251
                     /gene="cueR"
                     /locus_tag="L2X49_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBN4795326.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(62252..62710)
                     /locus_tag="L2X49_000174"
     CDS             complement(62252..62710)
                     /locus_tag="L2X49_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBN4795327.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(62707..63624)
                     /locus_tag="L2X49_000175"
     CDS             complement(62707..63624)
                     /locus_tag="L2X49_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBN4795328.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            63770..64447
                     /gene="fetA"
                     /locus_tag="L2X49_000176"
     CDS             63770..64447
                     /gene="fetA"
                     /locus_tag="L2X49_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBN4795329.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKDIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            64434..65213
                     /gene="fetB"
                     /locus_tag="L2X49_000177"
     CDS             64434..65213
                     /gene="fetB"
                     /locus_tag="L2X49_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBN4795330.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFVAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(65276..66130)
                     /gene="cnoX"
                     /locus_tag="L2X49_000178"
     CDS             complement(65276..66130)
                     /gene="cnoX"
                     /locus_tag="L2X49_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBN4795331.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(66191..67000)
                     /gene="ybbO"
                     /locus_tag="L2X49_000179"
     CDS             complement(66191..67000)
                     /gene="ybbO"
                     /locus_tag="L2X49_000179"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBN4795332.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(66990..67613)
                     /gene="tesA"
                     /locus_tag="L2X49_000180"
     CDS             complement(66990..67613)
                     /gene="tesA"
                     /locus_tag="L2X49_000180"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HBN4795333.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            67584..68270
                     /locus_tag="L2X49_000181"
     CDS             67584..68270
                     /locus_tag="L2X49_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN4795334.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            68267..70681
                     /locus_tag="L2X49_000182"
     CDS             68267..70681
                     /locus_tag="L2X49_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4795335.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(71393..72487)
                     /gene="mnmH"
                     /locus_tag="L2X49_000183"
     CDS             complement(71393..72487)
                     /gene="mnmH"
                     /locus_tag="L2X49_000183"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBN4795336.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEHGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEVGINYPLVEGGYKVLRQTAIQATIELSQKPIVLIGGCTGCGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(72556..73482)
                     /gene="allS"
                     /locus_tag="L2X49_000184"
     CDS             complement(72556..73482)
                     /gene="allS"
                     /locus_tag="L2X49_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBN4795337.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            73712..74194
                     /gene="allA"
                     /locus_tag="L2X49_000185"
     CDS             73712..74194
                     /gene="allA"
                     /locus_tag="L2X49_000185"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBN4795338.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWLRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            74272..75087
                     /gene="allR"
                     /locus_tag="L2X49_000186"
     CDS             74272..75087
                     /gene="allR"
                     /locus_tag="L2X49_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBN4795339.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            75177..76958
                     /gene="gcl"
                     /locus_tag="L2X49_000187"
     CDS             75177..76958
                     /gene="gcl"
                     /locus_tag="L2X49_000187"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBN4795340.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            76971..77747
                     /gene="hyi"
                     /locus_tag="L2X49_000188"
     CDS             76971..77747
                     /gene="hyi"
                     /locus_tag="L2X49_000188"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBN4795341.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            77847..78725
                     /gene="glxR"
                     /locus_tag="L2X49_000189"
     CDS             77847..78725
                     /gene="glxR"
                     /locus_tag="L2X49_000189"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBN4795342.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            78894..80348
                     /locus_tag="L2X49_000190"
     CDS             78894..80348
                     /locus_tag="L2X49_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HBN4795343.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            80408..81769
                     /gene="allB"
                     /locus_tag="L2X49_000191"
     CDS             80408..81769
                     /gene="allB"
                     /locus_tag="L2X49_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HBN4795344.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            81826..83127
                     /locus_tag="L2X49_000192"
     CDS             81826..83127
                     /locus_tag="L2X49_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HBN4795345.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            83149..84294
                     /gene="glxK"
                     /locus_tag="L2X49_000193"
     CDS             83149..84294
                     /gene="glxK"
                     /locus_tag="L2X49_000193"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HBN4795346.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(84522..85307)
                     /gene="allE"
                     /locus_tag="L2X49_000194"
     CDS             complement(84522..85307)
                     /gene="allE"
                     /locus_tag="L2X49_000194"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBN4795347.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(85318..86553)
                     /gene="allC"
                     /locus_tag="L2X49_000195"
     CDS             complement(85318..86553)
                     /gene="allC"
                     /locus_tag="L2X49_000195"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBN4795348.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMIRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAVVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(86575..87624)
                     /gene="allD"
                     /locus_tag="L2X49_000196"
     CDS             complement(86575..87624)
                     /gene="allD"
                     /locus_tag="L2X49_000196"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HBN4795349.1"
                     /translation="MKINRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIADDIYQYLISD
                     ALYNTSYETKNPFAQ"
BASE COUNT        21224 a        22047 c        23385 g        21053 t
ORIGIN      
        1 ggcgtcaata atttccaaac gctaagccgc acaaaagaac aaatattatt aagcgatact
       61 gatgtttatt tagccgtaat aattacggcg agtgattact acagctaaat aatatttaca
      121 gattacgtca gatgcgtttt tcgtagcgcc agataatcca gcaaaccacc aagcacgacg
      181 ccagaaaggg cgactaaaac cccgacttcc agcagagcag gcaggaaaga gaagtgcgtc
      241 aaatccagcg cgtccatcgt caacagcagt aaccacacgg ccaacaggct gacgcccgcc
      301 gccagtgtcc acatggcaaa ggcataacct gccggatatt cggtacggga gataaagttg
      361 tcatttactc gtgtatattc aagcgtctgg cggcaaacca gcagcaaaat aagccccggt
      421 acggcagcgg cgacggagaa tagatagaat gtcgaccagc cgtgtgcttc aacaaaccaa
      481 cccgccacgg ggccgacata aactcgtcct acagcggaaa gcgctgagag cagggcaaat
      541 tgagtagcgg aaaatgactt attacacagc gtcattaaca gcgcgacaaa ggctgatgtg
      601 cccatcccgc cacagaggtt ttcgaaaaag acggctgcgc ccatgctgta gagatgctta
      661 tcagtaatcg acagcagcca gtaaccagcg ttagacgcac cttgtaaaat gccgaaaatc
      721 agcagcgccc ggaacagcga caggcgctgc atcaaaatcc caccgtacaa tgcgcccaca
      781 atggtcgcca gtaagccaag cgttttgttt accacgccta cttcacccgc atcaaacccg
      841 acgccgcgaa tcaaaaacgt ggttgtcagg ctcatggcga atgcgtcgcc cagcttatac
      901 agcacgataa gaagcaaaat aagccaggca ttattgcgac caaagaaatc tcgcagaggt
      961 gcaacaaccg cttgttccag cgttttgggc acaggaatgg tgtcggttgg ttctggtgca
     1021 agcaacgtcg cgataataca ggggatcaac agcgccgcca tcaaccagta catgccctgc
     1081 cagcccagcc atttatctgc cagccacagg gctaggccgc cggaaaccag catccctaaa
     1141 cggtaaccca gcacgctgat tgccgcgccc gcaccacgct cttctgccgg gagcacatcg
     1201 gttttccacg catcgaagac aatatcctga gaggccgagc aaaaagcgat caccaccgcc
     1261 agcgccgcca tccagcggag ttgggtgcct ggttcgagaa aacccatcgc cgcaatggcg
     1321 actaataaca ggatttgcgt ggcgagcagc caaccgcgcc gccgcccaaa aaatgggggc
     1381 gtgtagcggt ccatcagcgg tgaccagagg aatttaaaaa cgtaagcctg gcctaccaga
     1441 gagaagaaac caatggtttt gagatcaata ttctcgaccg tcatccaggc ctgtaaggta
     1501 ccggatgtca gggcgagcgg tagcccggaa gcaaaaccca ggatcagcaa tatggctgaa
     1561 cgcggctgtt gaaaaatacg taaatattga ctggacatgg gcgtctacag gcccggcttg
     1621 caccgggcca gagggtagaa attaacgcgc gttctgcttg atgaattcgt ggatgctggt
     1681 gtcctgagac atatcagcga tggtatcggt cagcacactg ttaaccgcat cggcgatatt
     1741 tttgttggag gcctggaacg caccttcaac gttgtagctg gcacgatagt ttttggtcat
     1801 tttgttgcca ttctgcgcgg tagcgatgat ggcgatatcc gctttggtcg cgatgttgta
     1861 gcgcacgttg ccctgggaca cgtcagcata cagttggcta acgatgattt gcagattaac
     1921 cgggccattc ggaccaacca tgtaaccacg cgcggtcatc tgtttttcca gcacttcttg
     1981 cagcaggaaa cgcagatcgc gggaggcggt cagggtaacg atttgattat cgcgggtgac
     2041 ttttgccagc gcctgatcgg tacgctgatc ggcaccatta atgcttacgg tgacgcccat
     2101 caggcttgga tcctgctgtg gcagtgtaat cgtcggggaa acttcaatag ttgttggcgg
     2161 ttttgcgcat cctgcaagca taaacagagc aactaacggg aagaggattt ttttgaacat
     2221 gttcgggctc tcagagactc ttaagcgtgt ttggtaaaaa ttcccgccat cataacattg
     2281 ccaacggcga ggggaagtgg gtaaggcatg taaattcatc atgttgacga aataatcgcc
     2341 cctggtaaaa gaaacactga tgcgaggcct gtgtttcaat ctttaaatca gtaaacttca
     2401 tacgcttgac ggaaaaacca ggacgaaacc taaatatttg ttgttaagct gcaatggaaa
     2461 cggtaaaagc ggctagtatt taaagggatg gatgacatct cagcgttgtc ggaggagata
     2521 tttcatgatg atacgtgagc ggatagaaga aaaattaagg gcggcgttcc aacccgtatt
     2581 cctcgaagta gtggatgaaa gctatcgtca caatgtccca gccggctctg aaagccattt
     2641 taaagttgtg ctggtcagcg atcgttttac gggtgaacgt tttctgaatc gtcatcgaat
     2701 gatttacagt actttagcgg aggaactctc tactaccgtt catgcgctgg ctctgcatac
     2761 ttacactatt aaggagtggg aagggttgca ggacaccgtc tttgcctctc ctccctgtcg
     2821 tggagcagga agcatcgcgt aaaaacgcat ttgcaactgt cggcgctttt ccagtatgtt
     2881 gctaaagatt ttatgaaaaa cggcctgcgg gccgttttgt tttgtctgga ttttgcgctt
     2941 tttgcccagc attcagacga aaattgcccg ggaattgtga aaaaatacgc gacagcgcgc
     3001 aataaccgtt ctcgactcat aaaagtgatg ccgctataat gccgcgtcct atttgaatgc
     3061 tttcgggatg attctggtaa cagggaatgt gattgattat aagaacatcc cggttccgcg
     3121 aagccaacaa cctgtgcttg cggggtaaga gttgaccgag cactgtgatt ttttgaggta
     3181 acaagatgca agtttcagtt gaaaccactc aaggccttgg ccgccgtgta acgattacta
     3241 tcgctgctga cagcatcgag accgctgtta aaagcgagct ggtcaacgtt gcgaaaaaag
     3301 tacgtattga cggcttccgc aaaggcaaag tgccaatgaa tatcgttgct cagcgttatg
     3361 gcgcgtctgt acgccaggac gttctgggtg acctgatgag ccgtaacttc attgacgcca
     3421 tcattaaaga aaaaatcaat ccggctggcg caccgactta tgttccgggc gaatacaagc
     3481 tgggtgaaga ctttacttac tctgtagagt ttgaagttta tccggaagtt gaactgcagg
     3541 gtctggaagc gatcgaagtt gaaaaaccga tcgttgaagt gaccgacgct gacgttgacg
     3601 gcatgctgga tactctgcgt aaacagcagg cgacctggaa agaaaaagac ggcgctgttg
     3661 aagcagaaga ccgcgtaacc atcgacttca ccggttctgt agacggcgaa gagttcgaag
     3721 gcggtaaagc gtctgatttc gtactggcga tgggccaggg tcgtatgatc ccgggctttg
     3781 aagacggtat caaaggccac aaagctggcg aagagttcac catcgacgtg accttcccgg
     3841 aagaatacca cgcagaaaac ctgaaaggta aagcagcgaa attcgctatc aacctgaaga
     3901 aagttgaaga gcgtgaactg ccggaactga ctgcagaatt catcaaacgt ttcggcgttg
     3961 aagatggttc cgtagaaggt ctgcgcgctg aagtgcgtaa aaacatggag cgcgagctga
     4021 agagcgccat ccgtaaccgc gttaagtctc aggcgatcga aggtctggta aaagctaacg
     4081 acatcgacgt accggctgcg ctgatcgaca gcgaaatcga cgttctgcgt cgccaggctg
     4141 cacagcgttt cggtggcaac gaaaaacaag ctctggaact gccgcgcgaa ctgttcgaag
     4201 aacaggctaa acgccgcgta gttgttggcc tgctgctggg cgaagttatc cgcaccaacg
     4261 agctgaaagc tgacgaagag cgcgtgaaag gcctgatcga agagatggct tctgcgtacg
     4321 aagatccgaa agaagttatc gagttctaca gcaaaaacaa agaactgatg gacaacatgc
     4381 gcaatgttgc tctggaagaa caggctgttg aagctgtact ggcgaaagcg aaagtgactg
     4441 aaaaagaaac cactttcaac gagctgatga accagcaggc gtaatttacg cagcataacg
     4501 cgctaaattc gcacaaaggc ccgtcaccgc caggtggtgg gctttttttt gtcatgaatt
     4561 ttgcatggaa ccgtgcgaaa agcctctttc ggtgttagcg taacaacaaa agattgttat
     4621 gcttgaaata tggtgatgcc gtacccataa cacagggact agctgataat ccgtccataa
     4681 ggttacaatc ggtacagcag gttttttcaa ttttatccag gagacggaaa tgtcatacag
     4741 cggcgaacga gataactttg caccccatat ggcgctggtg ccgatggtca ttgaacagac
     4801 ctcacgcggt gagcgctctt ttgatatcta ttctcgtcta cttaaggaac gcgtcatttt
     4861 tctgactggc caggttgaag accacatggc taacctgatt gtggcgcaga tgctgttcct
     4921 ggaagcggaa aacccagaaa aagatatcta tctgtacatt aactccccag gcggggtgat
     4981 cactgccggg atgtctatct atgacaccat gcagtttatc aagcctgatg tcagcaccat
     5041 ctgtatgggc caggcggcct cgatgggcgc tttcttgctg accgcagggg caaaaggtaa
     5101 acgtttttgc ctgccgaatt cgcgcgtgat gattcaccaa ccgttgggcg gctaccaggg
     5161 ccaggcgacc gatatcgaaa ttcatgcccg tgaaattctg aaagttaaag ggcgcatgaa
     5221 tgaacttatg gcgcttcata cgggtcaatc attagaacag attgaacgtg ataccgagcg
     5281 cgatcgcttc ctttccgccc ctgaagcggt ggaatacggt ctggtcgatt cgattctgac
     5341 ccatcgtaat tgatgccaga ggcgcaactg tgccgctata cttatccagg gcggcacaac
     5401 gctgtaagcg gcttgcgcct gagaatggca tttgcgtcgt cgtgtgcggc acaaagaaca
     5461 aagaagaggt tttgacccat gacagataaa cgcaaagatg gctcaggcaa attgctgtat
     5521 tgctcttttt gcggcaaaag ccagcatgaa gtgcgcaagc tgattgccgg tccatccgtg
     5581 tatatctgcg acgaatgtgt tgatttatgt aacgacatca ttcgcgaaga gattaaagaa
     5641 gttgcaccgc atcgtgaacg cagtgcgcta ccgacgccgc atgaaattcg caaccacctg
     5701 gacgattacg ttatcggcca ggaacaggcg aaaaaagtgc tggcggtcgc ggtatacaac
     5761 cattacaaac gtctgcgcaa cggcgatacc agcaatggcg tcgagttggg caaaagtaac
     5821 attctgctga tcggtccgac cggttccggt aaaacgctgc tggctgaaac gctggcgcgc
     5881 ctgctggatg ttccgttcac catggccgac gcgactacac tgaccgaagc cggttatgtg
     5941 ggtgaagacg ttgaaaacat cattcagaag ctgttgcaga aatgcgacta cgatgtccag
     6001 aaagcacagc gtggtattgt ctacatcgat gaaatcgaca agatttctcg taagtcagac
     6061 aacccgtcca ttacccgaga cgtttccggt gaaggcgtac agcaggcact gttgaaactg
     6121 atcgaaggta cggtagctgc tgttccaccg caaggtgggc gtaaacatcc gcagcaggaa
     6181 ttcttgcagg ttgatacctc taagatcctg tttatttgtg gcggtgcgtt tgccggtctg
     6241 gataaagtga tttcccatcg tgtagaaacc ggctccggca ttggttttgg cgcgacgata
     6301 aaagcgaagt ccgacaaagc aagcgaaggc gagctgctgg cgcaggttga accggaagat
     6361 ctgatcaagt ttggtctgat ccctgagttc attggtcgtc tgccggttgt cgcaacgttg
     6421 aatgaactga gcgaagaagc tctgattcag atcctcaaag agccgaaaaa cgccctgacc
     6481 aagcagtatc aggcgctgtt taatctggaa ggcgtggatc tggaattccg tgacgaggcg
     6541 ctggatgcta tcgctaagaa agcgatggcg cgtaaaaccg gtgcccgtgg cctgcgttcc
     6601 atcgtagaag ccgcactgct cgataccatg tacgatctgc cgtccatgga agacgtcgaa
     6661 aaagtggtta tcgacgagtc ggtaattgat ggtcaaagca aaccgttgct gatttatggc
     6721 aagccggaag cgcaacaggc atctggtgaa taattaacca ttcccataca attagttaac
     6781 caaaaagggg ggattttatc tcccctttaa tttttcctct attctcggcg ttgaatgtgg
     6841 gggaaacatc cccatatact gacgtacatg ttaatagatg gcgtgaagca cagtcgtgtc
     6901 atctgattac ctggcggaaa ttaaactaag agagagctct atgaatcctg agcgttctga
     6961 acgcattgaa atccccgtat tgccgctgcg cgatgtggtg gtttatccgc acatggtcat
     7021 ccccttattt gtcgggcggg aaaaatctat ccgttgtctg gaagcggcga tggaccatga
     7081 taaaaaaatt atgctggtcg cgcagaaaga agcttcaacg gatgagccgg gtgtaaacga
     7141 tcttttcacc gtcgggaccg tggcctctat attgcagatg ctgaagctgc ctgacggcac
     7201 cgtcaaagtg ctggtcgagg ggttacagcg cgcgcgtatt tctgcgctct ctgacaatgg
     7261 cgaacacttt tctgcgaagg cggagtatct ggagtcgccg accattgatg agcgagaaca
     7321 ggaagtgctg gtgcgtactg caatcagcca gttcgaaggc tacatcaagc tgaacaaaaa
     7381 aatcccacca gaagtgctga cgtcgttgaa tagcatcgac gatccggcgc gtctggcgga
     7441 taccattgct gcacatatgc cgctgaaact ggctgacaaa cagtccgttc tggagatgtc
     7501 cgacgttaac gaacgtctgg aatatctgat ggcaatgatg gaatcggaaa tcgatctgct
     7561 gcaggttgag aaacgcattc gcaaccgcgt taaaaagcag atggagaaat cccagcgaga
     7621 gtactatctg aacgagcaaa tgaaagctat tcagaaagaa ctcggtgaaa tggacgacgc
     7681 gccggacgaa aacgaagccc tgaagcgcaa aatcgacgcg gcgaagatgc cgaaagaagc
     7741 aaaagagaaa gcggaagcag agttgcagaa gctgaaaatg atgtctccga tgtcggcaga
     7801 agcgaccgta gtgcgtggtt atatcgactg gatggtacag gtaccgtgga atgcgcgcag
     7861 caaggtcaaa aaagacctgc gtcaggcgca ggaaatcctt gataccgacc attatggtct
     7921 ggagcgcgtg aaagatcgca tccttgagta ccttgcggtt caaagccgtg tcaacaaaat
     7981 caagggaccg atcctctgcc tggtagggcc gccgggggta ggtaaaacct ctcttggtca
     8041 gtccatcgcc aaagccaccg ggcgtaaata tgtccgtatg gcgctgggcg gcgtgcgtga
     8101 tgaagcggaa atccgtggtc accgccgtac ttacatcggt tctatgccgg gtaaactgat
     8161 ccagaaaatg gcgaaagtgg gcgtgaaaaa cccgctgttc ctgctcgatg agatcgacaa
     8221 aatgtcttct gacatgcgtg gcgatccggc ctccgcactg cttgaagtgc tggatccaga
     8281 gcagaacgta gcgttcagcg accactacct ggaagtggat tacgatctca gcgacgtgat
     8341 gtttgtcgcg acgtcgaact ccatgaacat tccggcaccg ctgctggatc gtatggaagt
     8401 gattcgcctc tccggttata ccgaagatga aaaactgaac atcgccaaac gtcacctgct
     8461 gccgaagcag attgaacgta atgcactgaa aaaaggtgag ctgaccgtcg acgatagcgc
     8521 cattatcggc attattcgtt actacacccg tgaggcgggc gtgcgtggtc tggagcgtga
     8581 aatctccaaa ctgtgccgca aagcggttaa gcagttactg ctcgataagt cattaaaaca
     8641 tatcgaaatt aacggcgata acctgcatga ctacctcggt gttcagcgtt tcgactatgg
     8701 tcgcgctgat aacgaaaacc gtgtcggtca ggtaaccggt ctggcgtgga cggaagtggg
     8761 cggtgacttg ctgaccattg aaaccgcatg tgttccgggt aaaggcaaac tgacctatac
     8821 cggttcgctc ggcgaagtga tgcaggagtc tattcaggcg gcgttaacgg tggttcgtgc
     8881 gcgtgcggaa aaactgggga tcaaccctga tttttacgaa aaacgtgaca tccacgtcca
     8941 cgtaccggaa ggtgcgacgc cgaaagatgg tccgagtgcc ggtattgcta tgtgcaccgc
     9001 gctggtttct tgcctgaccg gtaacccggt tcgtgccgat gtggcaatga ccggtgagat
     9061 cactctgcgt ggtcaggtac tgccgatcgg tggtttgaaa gaaaaactcc tggcagcgca
     9121 tcgcggcggg attaaaacag tgctaattcc gttcgaaaat aaacgcgatc tggaagagat
     9181 tcctgacaac gtaattgccg atctggacat tcatcctgtg aagcgcattg aggaagttct
     9241 gactctggcg ctgcaaaatg aaccgtctgg catgcaggtt gtgactgcaa aatagtgacc
     9301 tcgcgcaaaa tgcactaata aaaacagggc tggcaggcta attcgggctt gccagccttt
     9361 ttttgtctcg ctaagttaga tggcggatcg ggcttgccct tattaagggg tgttgtaagg
     9421 ggatggctgg cctgatataa ctgctgcgcg ttcgtacctt gaaggattca agtgcgatat
     9481 aaattataaa gaggaagaga agagtgaata aatctcaatt gatcgacaag attgctgcag
     9541 gggctgatat ctctaaagct gcggctggcc gtgcgttaga tgctattatt gcttccgtaa
     9601 ctgaatctct gaaagaaggg gatgatgtag cactggtagg ttttggtact tttgccgtta
     9661 aagagcgtgc tgcccgtact ggccgcaacc cgcagaccgg taaagagatc accatcgctg
     9721 ctgctaaagt accgagcttc cgtgcaggta aagcactgaa agacgcggta aactaagcgt
     9781 tgtccccggt ggggatgtga cgaagttcaa gggcgcatct actgatgtgc cttttttatt
     9841 tgtattcggt gactttctgc gtcttgtggg ctgacaattg cccccgtttc ttgtcacaat
     9901 aggcctttgc gcgcatcgat acgttgcgtg aggtacacag tcatctacag cggagtgttg
     9961 ttacaccatg atggacagct tacgcacggc tgcaaacagt ctcgtgctca agattatttt
    10021 cggtatcatt atcgtgtcgt tcatattgac cggcgtgagt ggttacctga ttggcggagg
    10081 caataactac gccgcaaaag tgaatgacca ggaaatcagc cgtgggcagt tcgagaacgc
    10141 ctttaacagc gagcgtaatc gcatgcagca acagctgggc gatcaatact ccgagctggc
    10201 agcgaacgaa ggctatatga aaaccctgcg tcaacaggtg ctgaatcgtc tgatcgacga
    10261 ggcgctgttg gatcagtacg ctcgtgagct gaaactgggt atcagtgatg agcaggttaa
    10321 acaggcgatt ttcgcgaccc cagccttcca ggttgacggc aaatttgata acagccgcta
    10381 taacggtatc ctcaaccaga tggggatgac cgccgatcag tacgcccagg cgctgcgtaa
    10441 ccagctcact acccaacagc tgattaacgg tgttgccggt accgatttta tgctgaaagg
    10501 tgaaaccgac gagctggcgg cactggtcgc gcaacaacgc gtggtgcgtg aggcgactat
    10561 cgatgttaac gcgctggcgg cgaagcagcc tgtgaccgaa caggagattg ccagctacta
    10621 cgaacaaaac aaaaacaatt tcatgacgcc ggaacaattc cgcgtgagtt acatcaagct
    10681 ggatgccgca acgatgcagc aaccggttag cgatgcggat atccagagct actacgatca
    10741 gcatcaggat caattcaccc agccgcagcg tacccgctac agcatcatcc agaccaaaac
    10801 tgaagatgaa gcgaaagcgg tacttgatga gctgaataaa ggcggtgatt ttgctgcatt
    10861 agccaaagaa aaatctgccg atattatctc tgcccgtaac ggcggcgata tgggttggtt
    10921 agaagatgcc actatccccg acgaactgaa aaatgctggt ctgaaagaaa aagggcaact
    10981 gtctggtgtc atcaaatctt cggtcggttt cctgattgta cgtctggacg acattcagcc
    11041 agcgaaagtg aaatcgttag acgaagtacg tgacgacatt gcggcgaaag tgaaacacga
    11101 aaaagccctc gatgcgtact atgcgctgca gcagaaagtg agcgatgcgg caagcaacga
    11161 caccgagtct ctggccggtg cagagcaaac tgccggcgtt aaagccactc agacgggttg
    11221 gttcagcaaa gataacctgc cggaagagtt gaacttcaag ccggttgccg acgctatctt
    11281 taacggcggt ctggtaggtg aaaacggcgc gccgggcatc aactctgaca tcatcaccgt
    11341 agacggcgac cgcgcattcg tgctgcgcat cagcgagcac aaaccggaag cggtgaaacc
    11401 gttggcagat gttcaggaac aagttaaggc attggttcag cacaacaaag ctgaacaaca
    11461 ggcgaaagtg gatgctgaga aactgctggt tgatttgaaa gccggcaaag gtgcggaagc
    11521 tatgcaggct gccggtctga aatttggcga gccgaaaacc ttaagccgtt ccggtcgtga
    11581 cccgattagc caggcggcgt ttgcactgcc actgccagcg aaagacaaac cgagctacgg
    11641 tatggcgacc gatatgcaag gtaatgtggt tctgctggcg ctggatgaag tgaaacaagg
    11701 ttcaatgccg gaagatcaga aaaaagcgat ggtgcagggt atcacccaga acaacgcaca
    11761 aatcgtcttt gaagctctga tgagtaacct gcgtaaagag gcgaaaatca aaattggcga
    11821 tgcgctggaa cagcaataat cctgaagccg cctcgcaaaa aaatgcgttg cagctgtaac
    11881 aagaaaaggt cgctttcgcg gccttttcca tttctgaaca ttgccatttg tttactgttt
    11941 tcactgccgt taaggtgatt ccactgttaa caaacaagga gaaaacagta tgaaacacgg
    12001 aattaaagca ctgctcatta ccctgtctct ggcttgtgcc ggaatgtctc atagcgcgct
    12061 ggcggcagct cctgcggcga aaccgacaac ggtagaaacc aaagcggaag ctcctgcagc
    12121 acaaagtaaa gcagcagtac cggcgaaagc cagtgacgaa gaaggcaccc gggtcagcat
    12181 taataatgcc agcgcggaag agctagcccg cgcgatgaat ggcgttggcc tgaagaaagc
    12241 gcaggcgatt gtcagttatc gcgaagagta cggtccgttt aaaacggtgg aggatctaaa
    12301 gcaggtgccg gggatgggca attcgctggt ggaacgtaat ctggcggtat taaccctgta
    12361 attaatttgc atagtggcaa ttttttgcca gactgaagag gtcataccag ttatgacctc
    12421 tgtacttata acaacaacgt aaggttattg cgctatgcaa acacaaatca aagttcgtgg
    12481 atatcatctc gacgtttacc agcacgtcaa caacgcccgc taccttgaat ttctcgaaga
    12541 agcccgctgg gatgggttgg aaaatagcga cagttttcag tggatgacgg cccataacat
    12601 cgccttcgtc gtggtcaata tcaatattaa ctatcgtcgc ccagcggtat taagtgacct
    12661 gttaactatt accagtcagt tgcagcaatt aaacggtaaa agcggcatct taagccaggt
    12721 cattacactg gagccggaag ggcaggtggt agcggatgcg cttattacgt ttgtttgtat
    12781 tgatcttaaa acgcagaaag cattagctct ggaaggggaa ttgcgcgaaa agctggagca
    12841 gatggttaag taaacgtttt gtggtgccgg atgctcaagc cgcatccggc gacacccgga
    12901 ataattacct caacccggtt ttctgcttca tcgctgccat caccgtcggt ttatcggcca
    12961 gataatgatt caaaccgttg gcgcgtaaat tacatgccgc acaatgaccg caaccgtcgc
    13021 ctttaatgcc gttatagcag gtcaacgttt cgttacggac taaatccagt ttgccgtaat
    13081 aatctgccag cgcccaggtt tccgctttat caatccacat cagcggcgtt tcaaaacgaa
    13141 tatctttcgc catgcccaaa ctgacggcat ggtttagtgc tttcacaaac tcatcgcggc
    13201 aatccgggta gccggagaaa tccgtttcac agacgccggt aattacggct tctgctttta
    13261 cctgatacgc atatattgcc gccagcgtca ggaacaaaat attacgccct gggacaaacg
    13321 tattcgggat gccatcggct tcaggttcat aatcaggcac cggaatgctg tcacgtgtca
    13381 ggctgctgac cgccagctcg ttgagcagcg ttacatcaag aaccttatgc gcgcgtgctc
    13441 ccagtttcag cgccagttcg cgtgccacgt cgatttctgc gcgatgtcgc tgaccgtaat
    13501 cgaacgtcac gcaatggact tcatcatatt gttgtaatgc ctgcaccaga caggtggtgg
    13561 aatcctggcc tccactgaac acaacgacag cacgtttcat agataatcct gcttgaacaa
    13621 taaaagcgtt atggtaacgc ctgcgattaa cccggaccag ctattcattg cgacggcgcg
    13681 ggaagccagg cttcggtaaa ttcaaaccag ccgcgcggtg tcagtcgcac accgttcacg
    13741 ccgggagggg cactaatgcg atagtgatag ttgaactgcg gcgtcagcgt cgcgtctgcc
    13801 attaactggc taaaaaccgc tttcagggca ttaaatcggt tttcctcatc aggcattact
    13861 tgcaccgcgt ccagcgtcga ttgcagatgt gcatatgctg gagcgtcgaa aacatgtggc
    13921 catagtggat cgcaacgtag ccattgctcc agagtatatt ccggtgcttc gccaattaat
    13981 ctgtcgccca tcatgaggtc tgcgtgtgcc agtagggtcg tgtcgtccca gttttttgcg
    14041 ttatgaaaaa taattgtgag ttcacagcct tccgctgcca gtgtcgcctg taggcgttct
    14101 gccatggtat gaagttctat cggtaagtga taaaccagcg tcaaggtttt cggtagtttg
    14161 acttcatccg gtacttgcca gtgcggaata gtccagcctg gcagtaatgc atgactggcg
    14221 gtgatcaggt tttctccgac ttctaacgtt tgtaataaac cggattgatg aataatggag
    14281 atcacttttc gcgcctgcca gagggagagt cggggacttt tgcgcaacgt taaatagcaa
    14341 aaacctaaac tgatgccact actgacctgg ctgacccgtt gcagctcctc cggtttgccg
    14401 atggtgattt gcacgggatg ccgacaactg gttcccaaat ctttttcgaa aagcggcgga
    14461 gttatccagt actcaaccgc tttaagcagc ggatgacgca aatggtaata atcatggctc
    14521 tccaggcgca ccagctccgc tgtgaattgt gttaagcgaa aaggaccagt gccgatcagt
    14581 gggaattgcg gatgcgccag atggctgcaa tagctcgcca gccggtgcgc aagccagtaa
    14641 tcggggcgat gtaaaaagaa ggtcagacac tgcggatggg tgacttcaat acgcttcacg
    14701 ctaataaata attgatccag tgctggcagt tgtaacagca tcaataatcg ctggtgtaag
    14761 tgtgaggctt ttactgcatc gccgttatgc cagtgtaggg ttgaacgaag ataaaagtcc
    14821 cagcgtaacc cgtcagtaga ggtttcccag tgatgcgcta aatcgccaat cgggcgctga
    14881 gtattattat cgaagcgggt caggccggaa aatatctgcc cggcgagatg ctgctcggca
    14941 cggccgggca aaaagcctgg ttgtagcggt tcgagcgggc gatagtaggg aatacgcaac
    15001 gtgggtgtat cgttttgcca ttgtccgccc ataaacggct gtaacagagt gcgcagctca
    15061 cctggggcca gttgcgccag ctccagcaca tcttgctgct ttccggtttc cagtgcctgt
    15121 tccatcatcg cattgcgtag cgattccggc gtgaccagaa agcgtaattg tccgcgcttt
    15181 ccgcgtcctg actgcgcctg ccactccagc catcccgcct cctgtgcctg acgcaacagc
    15241 gtacgaacat ggcgttcgct gcaaaaacag cgttcggcca gttcgctgac ggtgacggtt
    15301 tgcggctttc cggctgaagg ttgccacaga cgttgatact ggttaagacg gttgagcaat
    15361 cgcatataaa cccggaacaa tattatttaa ctattcacta ttacttccgt atatatcagg
    15421 tgatactcaa tcaccattaa ccgtgtcaca gagtggagaa agaaatggct cgtctggcag
    15481 cgtttgatat ggatggcact ttattgatgc ctgaccatca tttaggtgag aaaaccctct
    15541 ctactttggc gcgactgcgt gaacgcgaca ttaccctcac ttttgccacg gggcgtcatg
    15601 cgctggagat gcagcatatt ctcggggcgc tatcgctgga tgcatatttg attaccggca
    15661 acggaacgcg cgtgcattct ctggaaggtg aacttttaca tcgtgatgat ttacctgcgg
    15721 atgtcgcgga gctggtgctg tatcagcaat gggatacccg agccagcatg catatcttca
    15781 atgacgacgg ttggtttacc gggaaagaga tccctgcgtt gttgcaggca tttgtctata
    15841 gcggttttcg ttatcagata attgatgtca aaaaaatgcc actcggcagc gtcaccaaga
    15901 tctgcttctg tggcgatcac gacgatctta cacgcttgca gatccagcta tacgaagcat
    15961 taggcgagcg tgcacatttg tgtttttccg ccacggattg cctcgaagtg ctgccggtgg
    16021 gctgcaataa aggcgctgca ttgacggtgc tgacccaaca tttaggttta tcgttgcgcg
    16081 attgcatggc ctttggtgat gcgatgaacg atcgcgaaat gttaggcagc gtcggtagcg
    16141 gctttattat gggcaatgcg atgccgcaac tgcgcgcgga gctcccgcat ttaccggtga
    16201 ttggacattg ccgaaatcag gctgtctctc actatttgac gcactggctg gactatccac
    16261 atctacctta ttcccccgaa taacgagatc ccttccagca ccgggcaatt gcccggtttt
    16321 ttttgcgttg aatttgtcat tttgtgccgt ggtgtttaaa ccgcacagaa taaattgtcg
    16381 cgatttcacc tttaaaatag aattaaaaga gaaaaaaatt ctccgtggaa gggctatgtt
    16441 agataaaatt gaccgtaagc tgctggcctt actgcagcag gattgcaccc tctctttgca
    16501 ggcactggct gaagccgtta atctgacaac caccccttgc tggaagcgcc tgaaacggct
    16561 ggaggacgac ggtatcctta tcggcaaagt cgccctgctg gatccggaaa aaataggcct
    16621 cggcctgacc gcctttgtgc tgataaaaac gcaacatcac agcagcgaat ggtattgccg
    16681 ctttgtcacg gtggttaccg aaatgccaga agtgctgggg ttctggcgca tggctggtga
    16741 atacgattat ctgatgcgcg tccaggttgc cgacatgaaa cgctacgacg agttttataa
    16801 gcgtctggta aacagcgtgc cggggctgtc ggacgtcact tccagcttcg cgatggaaca
    16861 gattaaatac accacttctt tacccatcga ataaatatcc agaatcaggt caggacacaa
    16921 cgcgtgcgat tatttgctca attaagctgg tatttccgtc gggaatggcg tcgctatctc
    16981 ggggctgtcg ccttgcttgt cattatcgcg atgctgcaac tggttccgcc aaaagtggtt
    17041 ggtattgttg tcgatggcgt gacagaacaa cactttacta ccgggcagat cctgatgtgg
    17101 atcgccacca tggtgctgat tgccgttgtg gtttatctcc tgcgttacgt ctggcgggta
    17161 ttgctgtttg gtgcgtctta tcaactggct gttgaactgc gtgaagatta ttaccgtcag
    17221 ctaagccggc agcatcctga gttttacctg cgtcatcgca cgggtgacct catggctcgt
    17281 gcgactaatg acgtcgatcg cgtcgtgttt gccgccggag aaggggtgct gacgctggtg
    17341 gattcactgg taatgggctg cgctgtgttg attatgatgt ctacgcaaat tagctggcag
    17401 ttgaccttat ttgccctgtt accgatgcca gtgatggcga tcatgattaa gcgcaacggc
    17461 gatgccttgc atgaacgctt taagctggca caggcggcgt tttctagtct taatgaccgc
    17521 acccaggaaa gcctcaccag tattcgcatg atcaaagcct ttggtctgga agatcgccag
    17581 tcggcgttat ttgccgcgga tgccgaagat accggcaaaa aaaacatgcg ggtggcgcgt
    17641 attgatgctc gtttcgaccc gaccatctat atcgcgattg gtatggcgaa cttgctggcg
    17701 attggcggcg gtagttggat ggtggtgcag ggcagtttaa cgctgggcca gctcaccagt
    17761 tttatgatgt atttaggtct gatgatttgg ccaatgctgg cgctggcatg gatgtttaac
    17821 attgtggaac gtggtagtgc tgcgtacagc cgtattcgcg cgatgctggc ggaagcgccg
    17881 gtggtgaacg atggtagcga acccgtgccg gaagggcgtg gcgaactgga tgtaaatatt
    17941 caccagttca cgtatccgca gactgaccat cctgcgctgg aaaacgtcaa tttcgccctg
    18001 aaacccggtc agatgctggg tatttgcggg ccgactggtt ccggcaaaag taccctgttg
    18061 tcgctcattc agcgtcattt cgacgtcagc gagggggata ttcgctttca tgatattcct
    18121 ctgacgaagt tacaactcga tagctggcgt agccgcctgg cggtggtcag ccagacgcca
    18181 ttcctttttt ctgacaccgt ggcgaataac atcgcgctgg gttgcccgaa tgccacccag
    18241 caagagattg aacacgtagc gcggttagcc agcgtacatg acgatatttt gcgtctgccg
    18301 caaggttacg atacagaagt gggcgagcgc ggcgtgatgc tttccggtgg gcaaaaacag
    18361 cgtatctcca ttgctcgagc gttattagtc aacgcggaaa tcctcatcct tgatgatgcg
    18421 ctttcggcgg tggacggacg cacagagcac cagatcctgc ataacctgcg tcagtgggga
    18481 cagggaagaa cggtaatcat cagtgcccat cgcctttcgg cactgacgga agccagtgaa
    18541 attattgtga tgcagcacgg acatatcgcc cagcgtggca atcatgatga gctggcgcaa
    18601 caaagcggct ggtatcgcga tatgtatcgc tatcaacaac tggaggcggc gctcgacgac
    18661 gttccggaaa ttcgcgagga ggccgtcgat gcgtagtttt agccaactgt ggccgactct
    18721 caagcgtctg ttagcgtacg gttcgccgtg gcggaaaccg ctggggattg cggtcctgat
    18781 gatgtgggtt gcggcagcgg cagaagtcag tgggccgctg cttatcagct attttatcga
    18841 caatatggta gcgaaaaata acctgccgtt gaaaatggtt gcagggctgg cggcggcgta
    18901 tgttgggcta caactgtttg ccgccgggct acattacgcg cagtcgctgc tgtttaatcg
    18961 ggcggcagta ggcgtagtgc aacagttgcg taccgacgtg atggatgctg cgttacgcca
    19021 gccattaagc gagtttgata cccaacccgt cgggcaggtg atttcccgcg tcactaatga
    19081 cactgaagtg atccgcgatc tctacgttac cgtagtggca actgtcctgc gtagcgccgc
    19141 actggtgggc gcgatgctgg tggcaatgtt cagcctcgac tggcgaatgg cgctggtggc
    19201 gataatgatt ttcccggtag tgctggtggt aatggtgata tatcaacgtt acagcacgcc
    19261 gattgtccgt cgtgtgcgcg cctatctggc ggatatcaac gacggcttta acgaaatcat
    19321 caatggcatg agcgttatcc agcagttccg tcagcaggcg cgatttggcg aacgtatggg
    19381 tgaggccagt cgttcacact atatggcgag gatgcaaacc ctgcgcctcg acggttttct
    19441 gctgcgtccg ctgctgagtc tgttttcatc gctcattctt tgtggcttgt tgatgctgtt
    19501 tggcttctcc gccagcggca ccattgaagt gggcgtgtta tatgcgttta tcagctatct
    19561 tgggcgactt aacgaaccct taatcgaact gaccacgcag caggcgatgc tgcaacaggc
    19621 tgttgttgct ggtgagcgcg tgtttgaact gatggacgga ccgcgccagc aatatggcaa
    19681 tgatgatcgc ccgttacaga gcggcaccat cgaagtcgat aacgtgtcat ttgcttatcg
    19741 cgatgacaat ctggtgctaa agaacattaa tctctctgtg ccttcgcgta actttgtggc
    19801 gctggtcggg cataccggca gtggcaaaag caccctcgcc agtttattga tgggctatta
    19861 cccgctaacg gaaggtgaga ttcgccttga tggtcgtcca ttaagttcgc taagtcacag
    19921 cgcgctgcgc cagggcgtgg caatggtgca gcaagatccg gtggtgctgg cggatacctt
    19981 cctcgccaac gtgacgctgg gacgtgatat ctccgaagaa cgcgtctggc aggcgctgga
    20041 aaccgtgcaa ctggcggagc tggcgcgcag catgagtgat ggtatttata cgccgctggg
    20101 cgagcaggga aataatctct cagtcgggca aaagcaactg ctggcactgg cgcgcgtgct
    20161 ggtggagacg ccgcaaatcc tgatccttga tgaggcaacc gccagcattg actccggtac
    20221 tgaacaggcg attcaacatg ctctggcggc ggtgcgtgaa cataccacgc tggtggtgat
    20281 tgctcaccgc ttatcaacta ttgttgatgc cgacaccatt ctggtgcttc atcgtggaca
    20341 agccgtggag cagggcactc accagcaact gctggcggcc cagggacgct actggcagat
    20401 gtatcaactg caacttgcgg gcgaagagct ggcagccagc gtgcgtgaag aggaatcatt
    20461 gagcgcctga atagcgcaat atttcatcgt tggtgcaaaa atgtaacgca ctgtgcactg
    20521 tcatagtgcg ttttcatttt caatcttctt aacttcctgc tctctttctc atttttcatt
    20581 tctggcacac cgcttgcaat atcttcttcg tgtagcagaa ccattactga attctgaccg
    20641 gaggggatct atgaagctgg tgaccgtgat aatcaaacca ttcaagctgg aagacgttcg
    20701 tgaagcgtta tcttccattg gtattcaggg cctgaccgtc accgaagtga aaggtttcgg
    20761 gcgtcagaaa gggcatgccg agctgtaccg gggggcggaa tacagcgtca acttcctgcc
    20821 aaaagtaaaa attgatgtgg cgattgctga tgaccaactc gatgaagtga tcgatatcgt
    20881 cagtaaggcg gcttacaccg gaaaaattgg cgacggcaaa atcttcgtcg ctgaattgca
    20941 acgcgtcatt cgtattcgta ccggcgaagc cgacgaagcg gcgctgtaat ctctggcaca
    21001 cagcaacagg aacgaaaaat gaagatagcg acgataaaaa ctgggcttgc ttcactggcg
    21061 atgctgccgg gactggtaat ggctgcacct gcggtggccg ataaagccga caatgcgttt
    21121 atgatgattt gtactgcgct ggtgctgttt atgactattc cggggattgc cctgttttac
    21181 ggtgggttga ttcgcggcaa aaacgtgctg tcgatgctga cgcaggtgac ggtgacgttt
    21241 gcactggtct gtattctctg ggtggtttac ggttactcgc tggcgtttgg tgagggcaac
    21301 aacttcttcg gcaacattaa ctggttgatg ctaaaaaaca tcgaactgac ggcggtgatg
    21361 ggcagcattt atcagtatat ccacgtggcg tttcagggct cgtttgcctg cattaccgtc
    21421 ggcttgatcg ttggggcgct ggcggaacga atccgcttct cagctgtgtt gattttcgtg
    21481 gtggtatggc tgacgctctc ttacattccg attgcgcata tggtgtgggg cggtggtttg
    21541 ctggcttctc acggtgcgct ggatttcgcg ggtggcacgg tggtgcatat taacgccgca
    21601 atcgccggtc tggtgggcgc gtatctgata ggcaaacgcg tgggcttcgg caaagaggcg
    21661 tttaaaccgc acaacctgcc aatggtcttc accggtactg ccattctcta tattggttgg
    21721 tttggcttta acgccgggtc agcgggcacg gcgaatgaaa tcgcggcact ggcatttgtg
    21781 aatactgtgg tcgcaacggc ggcggcaatt cttggctgga tcttcggtga atgggcgctg
    21841 cgtggtaagc cttctctgct gggagcgtgt tctggcgcga ttgccggtct ggtcggcgtg
    21901 acgccagcgt gtggctacat tggggttggc ggcgcgttga ttatcggcgt ggtagctggt
    21961 ctggcgggct tgtggggcgt tactatgctc aaacgcttgc tgcgggtgga tgatccctgc
    22021 gatgtcttcg gtgtgcacgg cgtttgtggc attgtcggct gtatcatgac tgggattttt
    22081 gccgccagtt cgctgggtgg cgtgggcttc gctgaaggtg tgacgatggg ccatcagttg
    22141 ctggtacagc tggaaagcat cgccattacg atcgtctggt ccggtgtggt ggcatttatt
    22201 ggctacaaac tggcggatct gacggttggc ctacgtgtgc cggaagaaca agagcgagaa
    22261 gggctggacg tcaacagcca cggcgagaat gcctataacg cgtaacaagc actgcaaaaa
    22321 atagccggac ggttttcacc tccggctatt tttttaattg tgattacgca ttaccccttc
    22381 ctgaacggtc gaggcaacca gcacgccgtc ctgggtataa aactcaccgc gcacaaagcc
    22441 gcgtgcgctg gacgccgagg tgctctccac gctatacagc agccattcat tcaaattaaa
    22501 cgggcgatgg aaccacatgg aatggtcaat ggtggcaatc tgaatccctg gttcgagaaa
    22561 accgatgccg tgcggctgta gagctaccgg caggaagtta agatcagaag cgtaaccgag
    22621 cagatactga tgaacgcgca ggtcatccgg cacgctacca tttgcgcgga tccacacctg
    22681 acgatgtggt tctgcgacgt gacctttcag tgggttatga aactccaccg gacggacttc
    22741 cagcggacga tcgcagatga atttatcttt aagcactggc ggcagcaggt gcgccagcga
    22801 ttgggcgatt tgcgtttccg aaggcaggcc atcaggcgct ggcgcggacg gcattgtttt
    22861 ttgatgttcg aaacccgctt ctggtgcctg gaaagaggcc gtcatataaa aaatcggttt
    22921 gccgttttga atagcagcaa cccggcgggc gctgaagctg ttaccgtcac gcagcgtttc
    22981 gacatcataa ataatcggct tcttactatc gccagggcga agaaagtagc tgtgaaacga
    23041 atgtaccagc cgctcttcag ggacggtctc ttttgcagca tacaaggcct gacccacgac
    23101 ctggccgcca aacacctggc gtaaacctaa atcttcactc tggccgcgaa agagtccttc
    23161 ctcaattttt tctagattta acaatgtcag taaatttttt agcgcctgac tcatataact
    23221 ctccagtaac aaagctgccg cagcaagcca aagtgagttg agtataacgc aaatttgcca
    23281 ctggtccgat gggtgcaatg gtctgaatta cgggctaatt acaggcagaa atgcgtgatg
    23341 tgtgccacac ttgttgacgt tactattttg ttaaccactc ttccggcgag gaaagttagc
    23401 ccgctggtgc attgataata aggagaaatg aatgaaactc gtgcacatgg ccagtggttt
    23461 agcggttgcg attgcattgg cggcttgcgc agataaaagc gcggatattc agacgccagc
    23521 cccggctgca aatacgtcta tttcagcaac acaacaacct gctatccagc aaccgaatgt
    23581 ctccggtacc gtctggatcc gtcagaaagt cgcactgccg cctgatgctg tgctgaccgt
    23641 gacactttct gacgcgtcgt tagccgatgc accgtcaaaa gtgctggcgc agaaagcggt
    23701 gcgtaccgaa ggtaaacagt caccattcag ctttgttctg ccatttaacc cggcagatgt
    23761 tcagccgaac gcgcgtattc tgttgagtgc ggcgattacc gtgaatgaca aactggtatt
    23821 tatcaccgat accgttcagc cggtgatcaa ccagggcgga actaaagccg acctgacatt
    23881 ggtgccggta cagcaaaccg ccgtgccggt tcaggccagc ggtggcgcaa cgactaccgt
    23941 accttcgact tcaccaactc aggtgaatcc gtcttcggca gttccagctc ctacgcaata
    24001 ttaagcgacg ttaaccctct ccgcccggag agggttagta gttccagcga taacgctgca
    24061 agtcgatttg cccgcttccc gataccatta caccttctgc cagtaatgcc tgtcgctgac
    24121 gctgtaaatc cggtccggtt agcgaaattg tgccgtggcg attaaccacc cggtgccagg
    24181 gtaaggtgct gccttcaggg agacgcttta acacaccgcc cacctggcgc gcggcgcggg
    24241 gcgatcccgc cagtttcgcc acatcaccgt aagtggtgac atagccttcg ggaatagcgg
    24301 cgacgatttg ccagacgcgt tggggaaatg aatcttcttt ttccatcttt ttttcctgag
    24361 gtaatttttc agcataatct ggaaaaacgc ccgagtgaag tcgcattgcg caagaaacca
    24421 gcatctggca cgcgatgggt tgcaattagc cggggcagca gtgataatgc gcctgcgcgt
    24481 tggttctcaa cgctctcaat gggggctctg ttggttctcc cgcaacgcta ctctgtttac
    24541 caggtcaggt ccggaaggaa gcagccaagg cagatgacgc gtgtgccggg atgtagctgg
    24601 cagggccccc acccatttct gcctcccacc gtttcgtcaa aaaaccccag catggctaaa
    24661 ctttaaccac aactgacgtc gcaagaattg tctggctgcg cagtacgctt cggaggtatg
    24721 tctgatgaag tatgttgatg gttttgtggt tgccgttcct gccgataaaa aggatgccta
    24781 tcgggaaatg gccgctaagg ctgcgccatt gtttaaagag tttggcgcgc ttcgtattgt
    24841 cgaatgctgg gccagcgatg taccggatgg caaagtgacc gattttcgta tggcggtgaa
    24901 agcggaagag aatgaagagg ttgtctttag ctggattgaa tacccttcaa aagaggtccg
    24961 cgacgctgct aatcaaaaga tgatgtcgga cccacggatg aaagagttcg gcgagtccat
    25021 gccgtttgac ggcaagcgaa tgatctatgg cggattcgag tcaatcatcg acgaataggt
    25081 agcgtgacgg gctgtgcggc gtgccgtcag cccaaaaatt cacaaatgtt gctcggccca
    25141 gcgtaagaaa tcttctttcg gtaatgcctt gctgtagagc cagccctgac cgtagtgcac
    25201 gccatgctgg cgtagccact cttcttgttt actggtttcg attccctccg ctaccatttt
    25261 cagtttcagt gtttttgcca tttcgatgat atgcggcgtg acatttttat attccagcgc
    25321 atcaacgaaa gatttatcga tcttcagaat gtcgacatcc agatcctgta aataacttaa
    25381 acttgaatat cccgtaccaa aatcatcaag ataaatttca tggcccgcct cccggtagcg
    25441 agaaattatc ggggcgctgg ttttcggatc ggcaaactcg cgttcagtga gttcaagcgc
    25501 gatctgtctg ggattaacct gatagtgatt gatcatttca cgcagcaatt gcgggatttt
    25561 ttccgaggtg agcacggtgg attcaagatt gatcgaaata tgctgctgtg gatgctgacg
    25621 cagccagtcg cccatatctt caaagacgct tcttataatc agtagcgtca atggctcaga
    25681 aaggcccgtt tgctgtgcca gcggaataaa actatctggt gacaaccaac taccgtctgt
    25741 ctgcggccag cgcgccagtg cctcagcacc gacaattttg ccattggcta aggagacaat
    25801 cggctgatag tgcacgcaaa tatcacgatt ttcgatagca tcctgcagcc gatgatgcgg
    25861 tgactggata cggcgcaaaa tacgcagcac aaacatcgct gccagcaggc caatcaccaa
    25921 cccgagcggt aaccaaataa agacttgccg atgccagcct ttctgcaaca ttttcgttga
    25981 agcccaggtg atgatcgaaa tattcatctc cgggaagggc aggatatcgt aaatgattcc
    26041 attattttcg atatgctcac cgggtgtttt ttgtagcctg gtaataattc cctgagcaat
    26101 ttcatcgctg ccggttatga caacgttatg ggcattgcca ataatggcgg catcaatttg
    26161 ccatgagcta tagggaatga catcaataaa ggaagcgggg tcgatcatga cgacataatg
    26221 tgccgttccc atggcgacca tgtaacggat aatgcctaaa tcgttatgcg atgttaacca
    26281 gacacgataa ccatctttcg aaattttacc tggctcgggg aaggtatcgg gcggactttc
    26341 atgctccaga gacgaacact ggggaacgtt gttatcgata taaaccactt cctgaatata
    26401 gcgataactg taagataccc gacgcatttc catgagatgg gcttcgctac aggcagcgcc
    26461 ttgccatctt tccagctctt gcagcgcatc tttcccttgt gtcgccacct tattggcccg
    26521 aatagcgacg cgggaggaat aggtatccag ctcttcaata aacgatgttt ctacctgctg
    26581 atgggccagc cagatgctta agccgacagg cagcaatact gaaagaatca gtactcccga
    26641 aataaggccg accagatgtc gtgttctcac cagcatatcc ttattaacac agtgtaggga
    26701 aagtatatct gattgtggaa aaaggctggc aggagataag gaggtagagc tggtatttca
    26761 tgaagtgatg ggtggaaacg atcattgtca tttgaccagg cgcagagctt tacgcctggt
    26821 atgtaaccct aacttaagat gttgattcgg cgcgagcgag ggtaaaaata tcgtaaaaag
    26881 acagttcacc tttacggacg atcatttttt gcagttgttc cttatgaaca tccgcgacac
    26941 gcggagagtt caagatggct tcaaccagcg cggcaggtac aaagcgcgtg ttgtaccatt
    27001 cgccgttata gcaaacgcgg aaatccagca cgtcgatatc ttcatagcgg tagcgtgggt
    27061 agacatcgaa aaagaagaaa ccattaagca ggataaataa tacaaccagt agccagacag
    27121 agcctgaaag cgtttcggac tgcaacatca ccgccagggt ggcaaaaaaa gcaacgtaca
    27181 taccgataaa cagccccgga tgtttacgga taaaactgat actaaagcgg ggtttgttgt
    27241 cgcgtttttc gcgggtattc agagactcaa tcgtttcggt cagcaggcgt tgtatttcgg
    27301 tcatcgtttg ccttcgtggt atctgcaact ttccagggaa caccctattt taacgtggga
    27361 atagtagagg ggaagtaaca gatttaccac aattttgcat aacagttgcg aaaaaccgat
    27421 tacaattttt taattattct ggctggatta ccgcccacga caacgttgtt cgggacatct
    27481 tttgtgacaa ctgcacctga ggctaccacg acgttatcac caatggtcac accagggtta
    27541 atgaccgcgc gtccgccaat ccagacgtta ttaccgatgg tgacgggttt ccccagttca
    27601 gcaccgctat tacgtgctac agggtcgatg ggatgtgttg ccgtgtagat atgaacgcct
    27661 ggtgccaaca tacagttatc accgatgcga atagggcaga catcaagcat cacgcaatcg
    27721 aagttggcga aaaaattatt accgagaaaa atgttatagc catagtcaca gcgaaacgtt
    27781 ggctcaatat aagcctctgt cacctgaccg aatagatcag cgagaatttg ctggcgtaat
    27841 gtgtgctctt ccgccaggga atgattgtat cggtgaataa gctgacgagc gcgcaggcga
    27901 tcgcgagata acgtctcatc tgccgagcga tacaactcac cagcaatcat cttttctttt
    27961 tctgtgctca ttactgacct ccggttgctg aaaatgcggc aacacagtaa gacgaaaagt
    28021 cagtgaagag aacgttccca ttacatctgt gtgataaaga tcacataggg tatgtcttca
    28081 tggcgaaaaa gtataaaatt cttaataaac agccggttat agctccgaaa gcgaattatt
    28141 tattagcgaa taaatttcca aactgaggaa gggatcttgt cgtacagttt attcatggtc
    28201 aattcggcga ggcggtgatc tgcggctgag taaaataccg ccagttcatt atctgataat
    28261 tcgtatttat ttttctcgat aacacgctcc agcgtgtcaa ttgtctggca acgacgtaaa
    28321 cgcattaaat aatcggtttt cgttaaaggt ttttcggaca tacttctacc catggttgta
    28381 ataattctaa caagataaac tcgcaggatt ctctttcgtc gcattgacaa aacaacggaa
    28441 tagtcgatta cctgacttcc gccatttctg aagatcctgc atattaatac cataactact
    28501 gaacaacata aaggtgtcat ccagatattc gtcgatctgc tcaatgagct tattgtcttc
    28561 attatactta attttgtaat taagtgcgaa ggtcgcaata tgctcaatca gttcattcaa
    28621 ctggaggttg atcgccgagg ttgggtcgtt aacccagcca tgattgcttt cttcaaggtt
    28681 tgcaaggcag tcatgataca gggtttcaca gagaaactta agctgtgcga tatcatgtct
    28741 tttgggtgag tattcatcca taacgcgtcc ccttcttagc ggttgaacta acggacacct
    28801 ttcgggatgg aaaaaactta ctgacctgga cttgccctct cgtttgttag cttaactata
    28861 agccactctt tgcaggtttt catcgcatta ttacgaaaaa ttacaattag gaaggaatcg
    28921 ggacaggact cactttttca tactagcact aacccgcagc aggtaaaagc agtttgctcg
    28981 aactgttcat aacatttcag gattatcgcc agatgaaatt ggtgacacta ataccaggat
    29041 tgctctgaat atgacgtaat aaccgaggaa tgaataaaga attaccgcaa ataattaaga
    29101 atagcctcta aatgattatg gattattaaa tctattagcg ctacttaata taaattaact
    29161 aacaatcagt attttttatg aattttatcc gtggttaata ctggttttcg tatgagatcc
    29221 tgagttggtg gttcaattac tccttaatgt tcgtaggtta tgcataaaaa aggccgcttg
    29281 cgcggcctta gtgattacac gttgtatcaa tgatgatcga cagtatggct gtgctcgata
    29341 tcttcattct tgcggctaaa gcggcggcga accacaacaa agaataccgg aacgaagaag
    29401 attgccagta ccgttgcggt caccatcccg cccattacac cggtacctac tgcgttctgc
    29461 gcgccggaac cagcaccagt actgataacc agcggcataa cgccgaggat aaacgccagc
    29521 gaggtcatca ggatcggacg taaacgcatc cgcactgcat caagcgtcgc ttcaatcaga
    29581 cctttacctt ctttatccat caagtctttg gcgaattcga cgataagtat cgcgttcttc
    29641 gccgacaacc caatggttgt gagcaggcct acctggaagt aaacgtcatt ggtcaggcca
    29701 cggaaggtgg cagccagcaa cgcaccgata acccccagcg gaacgaccag cataacggag
    29761 aacggaatcg accagctctc gtacagcgcc gccagacaca ggaatacgac aatcaacgaa
    29821 atcgcgtaca gtgaaggtgc ctggttgccg gagaggcgtt cctgatagga catccccgtc
    29881 cagtcatagc caacaccggt aggcagtttg ctcgccagtt gttccattag ctccattgct
    29941 tcaccggtac ttttacccgg tgccgcctgg cctaagattt ccatggatgg caggccattg
    30001 taacgttcca gacgcggcga accgtactcc caacgagaag aggagaatgc cgagaacggc
    30061 accatctgac catcagcagc acgaacatac cagtcgccga tatcatccgg cagcatacgg
    30121 tatttcgctt ctgacatgac gtaaactttc ttcacacgac cgcggtcgat aaagtcgttc
    30181 acatagctac cgccccatgc agcgcccaga gtggtgttaa tgtcgttgat agaaacaccc
    30241 agcgcctgcg ctttttcctg gtcgatatca atcttaaact gcggggtatc ttccagaccg
    30301 tttggacgta cgctggtcag catatcaggg tgcttcgctg cttctgcaag cagctggtta
    30361 cgcgcctgag tcagtttttc gtgaccaagg ccagcctggt caatcagctc aaagtcaaag
    30421 ccggttgcag tacccagttc cacgattgcg ggcaggttaa aggcgaaaac catcgcatct
    30481 ttgatttgcg agaaagcgcg tgttgcacgc atggtaatcg cttcaacttt gttttcttcg
    30541 cccggacgat cggcccagtc cttcaaggaa acgaacgcaa taccggtatt ctgaccacgt
    30601 cccgcaaagc cgaagccgtt aacggcgaac accgactcaa cgttgttctt ttctttggtc
    30661 agatagtaat gcgttacctc attgagcact ttctgcgtac gttcctgcgt tgcacctgct
    30721 ggcagctgaa ccatggtcat aaatacgccc tggtcctcat ctggcaagaa ggagcttggc
    30781 agacgcacga acagataggc catgccgacc acgatgatca gatacagcac caggtaacgc
    30841 cccgtactgc gcagaatacc gcctacgctg tcggtgtagt ggtgcgtgct cttctcgaac
    30901 atgcggttaa accagccgaa gaagcctttt ttaccttccc cgtgatcgcc tttggcaatc
    30961 ggtttcagca tggtggcaca aagagccgga gtcaggatca acgctaccag taccgacagc
    31021 gccattgctg aaacaatggt aatagagaac tgacgataga tagcaccagt agaaccgcca
    31081 aagaaggcca tcggtacgaa taccgccgac agtaccatcg cgataccgac cagagcgccc
    31141 tgaatctgcc ccatcgactt acgggtagct tcttttggcg gcaaaccttc ttccgccata
    31201 acacgctcca cgttttctac cacaacgata gcgtcatcca ccaacaagcc gatagcgagc
    31261 accatcccga acattgttag cgtgtttatc gagaagccaa aggcggcaag gacggcaaag
    31321 gtcccgagca ataccaccgg tacggcaatg gtcggaatca acgtcgcgcg gaagttctgc
    31381 aggaacagat acataaccag gaacacgagg atgatcgctt cgaccagcgt tttaaccact
    31441 tcgtgaatag agattttcac gaacggcgtg gtgtcgtacg ggtaaacaat tttcagaccc
    31501 gacgggaaga acggttccat cttcgccagt tcagcacgga ttgccgcagc ggtatccagc
    31561 gcgtttgcac cggtcgccag cttgatcccc agaccggaag ccggttggcc gttaaactct
    31621 gcgatgatgt cgtagttctc accacccagc tcaatcttcg ccacatcacg cagcagtacg
    31681 cgggaaccat cttgattcac tttcagcagg attttgccga actcttcagt agaggtcaga
    31741 cgcgtctgag caataataga ggcgttaagc tgttggcctt tcaccggcgg cgtaccaccg
    31801 agctgacccg ccgcaacctg ggcgttctgc gctttgatgg cggtaatgac atcaaccggc
    31861 gttagctgga atttgttcag ctcattcggg ttcatccaga tacgcatcgc gtactgtgaa
    31921 ccgaacaact gaacatcacc cacgcccgac gtacggctga tggcatcttt catattcgcc
    31981 gctacgtagt cagagatatc ctcctgcgtc atggtgccat cggtgttgat aacgccgaca
    32041 accatcagga agctgctgga tgatttctca acgctcaccc cttgctgctg aacttcttgc
    32101 ggcagcaacg gcatcgccag ctgcagtttg ttctgcacct gaacctgcgc gatgtccgca
    32161 tcagtaccag actcaaaggt cagggtgatc tgcacggtac ccgtggagtc actgttagag
    32221 gacatgtaca tcaggttatc gataccgttc atattctgtt cgataacctg tgtcaccgtg
    32281 tcctgcactg ttttcgcatc agcgccgggg taggaggcgg agatcgttac tgccggcggt
    32341 gcaatcgtag gatattgcgc caccggcagt ttgaggatcg ccagcccccc tgccaacatg
    32401 atgataatgg cgatcaccca cgcaaaaatc gggcgatcga taaagaaatt aggcatgtct
    32461 taacggctcc tgtttaagtt aagacttgga ctgttcaggc tgagcaccgc ttgcggcttg
    32521 ctggttatta tcagcggtaa cttcttgtgc ttttacctgg acaccaggac gcactttctg
    32581 cagcccactt attactacgc gatcgcctgc tttcagacct tctgtcacca gccacttatc
    32641 gccaatagcc tggcttgcaa cgatcggacg ggtttccact ttgtcatccg cgccaactac
    32701 cagtacggtg gcatcaccac gcggcgtacg ggttacgccc tgttgcggga ctaaaatagc
    32761 gtttggatta agcccttctt ccagacgtgc gcgcacgaac atacccggca gcagagtgtg
    32821 atccgggttc gggaagatag cgcgtagggt gatagaccca gtggtctgat caacggtaac
    32881 gtcagagaat tccagcgtac cgtcctgcgg gaacttaatg ccgtcactgg tgatcagcga
    32941 cactttggct ttgccgttct cttgtttcag cgtgccattc gccagttcct gtttcaggcg
    33001 caggaagtcg ttgctggact gggtcacatc aacgtagatc ggatcaagtt gctgcacggt
    33061 tgccagcgca gtcgcctgac cgttctgtac caatgcgcct tccgtcacgt tcgacttacc
    33121 aatgcgaccg ctaatcggag aggtgacttt ggtgtaagcc agattgatcc gcgcagtttc
    33181 aacggcagct ttcgccgcag ttaccgcagc attcgcctgt tgcgcatcag ccagagcctg
    33241 atcgtactct tgcttactga tgtactgagt accgagcagt ttctgataac gattcaccgt
    33301 caattgcgcg atattggctg cagcctgggc tttcgccaga tcacctttcg cactgtcgta
    33361 tgtcgcctga taggtcgcag gatcaatctg atagagagag acaccagctt cgatgtcgct
    33421 accttctttg aaattacgct tcaggataat cccgctaact tgaggacgaa cttctgcgat
    33481 ccggtaggca ctggtgcgac ccggaagctc ggttgtgatc tgcagaggtt cagttttgac
    33541 tgttactacg ccaacggcgg gcatctgctg gccaccttgt tgggcctgtt tgtcgtcaca
    33601 tcctgttagg gctaagctgc ctgagagcat cagaacgacc gccagaggcg taaaccctct
    33661 gtttttgttc atatgtaaac ctcgagtgtc cgatttcaaa ttggtcaatg gtcaaaagtt
    33721 aataaaccca ttgctgcgtt tatattatcg tcgtgctatg gtacatacat tcacaaatgt
    33781 atgtaaatct aacgcctgta aattcacgaa catatggcac gaaaaaccaa acaagaagcg
    33841 caagaaacgc gccaacacat cctcgatgtg gctctacgtc ttttctcaca gcagggggta
    33901 tcatccacct cgctgggcga gattgcaaaa gcagctggcg ttacgcgcgg tgcaatctac
    33961 tggcatttta aagacaagtc ggatttgttc agtgagatct gggaactgtc agaatccaat
    34021 attggtgaac tagagcttga gtatcaggca aaattccctg gcgatccact ctcagtatta
    34081 agagagatat taattcatgt tcttgaatcc acggtgacag aagaacgacg tcgattattg
    34141 atggagaata tattccacaa atgcgaattt gtcggagaaa tggctgttgt gcaacaggca
    34201 caacgtaatc tctgtctgga aagttatgac cgtatagaac aaacgttaaa acattgtatt
    34261 gaagcgaaaa tgttgcctgc ggatttaatg acgcgtcggg cagcaattat tatgcgcggc
    34321 tatatttccg gcctgatgga aaactggctc tttgccccgc aatcttttga tcttaaaaaa
    34381 gaagcccgcg attacgtcgc catcttactg gagatgtatc tcctgtgccc cacgcttcgt
    34441 aatcctgcca ctaacgaata accctgaatc tgactccagg atttttcctg gacattttcg
    34501 tcgttgctat tctggttcac tgcgtcgtga tattcttgcg gtttgacttt ttcaggtcgt
    34561 tcttcaggtt cagaaacctt cattcatcat gactatgttc cagtattaca aacgatcacg
    34621 gcattttgtt ttttcagcat ttattgcttt tgtttttgtc ttgttatgcc agaacacggc
    34681 gtttgcgcgg gcgtcatcga atggtgatct gccgacaaaa gcggacctgc aggcgcaact
    34741 tgactcacta aataaacaaa aagatctttc tgctcaggac aaactggtgc agcaggatct
    34801 gacagataca ttagccaccc tcgataaaat cgatcgcata aaagaagaga cagttcagct
    34861 acggcaaaaa gtcgctgaag cgccggaaaa aatgcgccag gcgaccgcgg cgttaacagc
    34921 acttagcgat gtcgataacg acgaagaaac gcgcaaaatt ctgagtacgc tttcgttgcg
    34981 ccagctggaa actcgcgttg cccaggcgct ggacgatttg caaaacgcac aaaacgatct
    35041 ggcgtcttat aacagccagc tggtttcgtt acagacgcag cccgaacgcg tgcaaaatgc
    35101 gatgtataac gcttcgcagc agctgcaaca aattcgcagt cgtctggatg ggactgatgt
    35161 cggcgagact gccttacgtc ccagccagaa agtgttaatg caggctcagc aggcgttgct
    35221 gaatgcggag attgaccagc agcgtaaaag cctggaaggg aacaccgtct tgcaggatac
    35281 cttgcaaaag caacgtgatt acgtgacggc gaacagcgcc cgtctggagc accagttgca
    35341 gctgttgcaa gaagcggtaa acagcaagcg cctgacttta actgaaaaaa cggcgcagga
    35401 agccgtctcc ccggatgaag ccgcgcgtat tcaggctaat ccgctggtga aacaggaact
    35461 ggaaattaac cagcagttaa gtcagcgtct gattaccgcg actgaaaacg gtaatcagtt
    35521 gatgcagcaa aacattaaag tcaaaaactg gctggagcgg gcgctgcaat cggaacgcaa
    35581 tattaaagag cagattgccg tcctgaaggg cagcctgctg ttgtctcgta tcctttacca
    35641 gcaacaacaa acgctgccct cggcggatga actggaaaac atgaccaacc gcatcgcgga
    35701 tttgcgtctc gaacagtttg aagttaacca gcagcgtgat gcactcttcc agagcgatgc
    35761 gttcgtcaac aaactggaag aaggtcacac caacgaagtc aacagcgaag ttcacgatgc
    35821 gttattgcaa gtggttgata tgcgtcgcga attgctggat caactcaaca aacagttggg
    35881 taaccagctg atgatggcca ttaacctgca aatcaaccag cagcagttaa tgagcgtatc
    35941 gaaaaacctg aaatccatcc tgactcagca aatcttttgg gtgaacagta accgtccaat
    36001 ggactgggac tggatcaaag cgttcccgca aagcctgaaa gatgaattta agtcgatgaa
    36061 aatcacggtg aactgggaaa aagcctggcc tgccgttttt atcgctttcc tcgccggttt
    36121 gccgctgctg ttgattgccg ggctgatcca ctggcgtctg ggctggctga aagcgtatca
    36181 acagaaactg gcttccgctg tgggttccct gcgtaacgat agccagctca acacaccaaa
    36241 agcgatcctt atcgacctga tccgcgcgct gccggtgtgc ctgattattc tcgcggttgg
    36301 cctgattctg ttgaccatgc agctcaacat cagcgaactg ctatggtcgt tcagtaaaaa
    36361 actggcgatt ttctggctgg tgtttggcct gtgctggaag gtactggaga aaaatggcgt
    36421 tgccgtgcgt cacttcggca tgccggagca gcagaccagc cactggcgtc ggcaaattgt
    36481 ccgcatcagt ctcgcgttgc tgcctatcca tttctggtct gtggtggcag aactttcccc
    36541 gctgcatctg atggatgatg tgctggggca ggcgatgatt ttcttcaacc tgctgctgat
    36601 tgccttcctg gtatggccaa tgtgccgcga aagctggcgt gataaagagt cgcacaccat
    36661 gcgactggtc accattaccg tgctgtcgat tatcccgatt gcattgatgg tgctgactgc
    36721 cacgggttat ttctacacca cgctgcgcct ctccgggcgt tggattgaaa ccgtttatct
    36781 ggtgatcatc tggaacttgc tctaccagac ggtattgcgc ggcttaagcg tagcggcgcg
    36841 gcgtatcgcc tggcgtcgtg cgctggcgcg tcggcagaat ctggtgaaag agggcgcaga
    36901 aggtgccgaa ccgccggaag aacccaccat tgcactggaa caggttaacc agcagacgct
    36961 gcgtattacc atgttgctga tgtttgcgct gttcggtgtc atgttctggg caatttggtc
    37021 cgatttgatc accgtgttca gctatctcga cagcatcacg ctctggcatt acaacggcac
    37081 tgaagctggc gctgcggtgg tgaaaaacgt caccatgggc agtctgttgt ttgcgattat
    37141 cgcctcaatg gtggcctggg cgttgattcg caacctgcct ggtttactgg aagtgctggt
    37201 gctctcgcga ctgaatatgc gtcagggcgc gtcgtatgcc attactacca tccttaacta
    37261 catcattatt gccgttggtg cgatgacggt gttcggatcg ctgggcgtct cttgggataa
    37321 actccagtgg ctggctgcag cattatccgt aggtcttggt tttggtttac aagaaatttt
    37381 cggtaacttc gtctccggtt tgatcattct gttcgaacgt ccggtgcgta ttggcgatac
    37441 ggtaaccatt ggtagcttct cggggacggt aagtaagatc cgtattcgtg cgacaacgat
    37501 taccgatttc gatcgcaaag aagtgatcat cccgaacaaa gcgtttgtta ccgagcgtct
    37561 gatcaactgg tcgttgactg acactactac gcgtctggtg atccgtctcg gcgtggccta
    37621 tggctccgat ctggaaaaag tgcgtaaagt gttactgaag gcggcgactg agcacccaag
    37681 ggtgatgcac gaaccaatgc cggaagtctt ctttacggcg tttggtgcca gcacgttgga
    37741 tcatgagctg cgtctgtatg tgcgtgaact gcgtgaccgt agtcgtactg tcgatgagct
    37801 gaaccgtact atcgatcagc tgtgccgtga aaacgacatc aacattgcct ttaaccagct
    37861 tgaagtgcat ctgcacaacg agaagggcga tgaggtgacg gaagtcaaac gcgactacaa
    37921 aggcgatgac ccgacgccag cgctagggta ataaccaaag ggggcgacat ttagttgccc
    37981 cgagattact gacaaagtgc gcgttgttca tgccggatgc ggcgtgaccg ccttatccgg
    38041 cctacgaaac cgcaagaatt caatgtattg caggagcggt gtaggcctga taagcgtagc
    38101 gcatcaggca gttttgcgtt tgcccacaat cttaggggac atttagcggc cccatttatt
    38161 tctcactttt ccgcctcatc atcgcgcgtt aatttctttt cataatcgcg ttttacaata
    38221 tccagcgcgc gcagcacggt gcgggctggg atctgatttt cttccagcag cacaatcaaa
    38281 tcgacagcca gtttgacatc gtcaggggca ttttccagtg acatactctc tccattgcta
    38341 gcgggttaag cgcgctagcc tgttttcgat tttttccagc gcatggcggc agcgtgccag
    38401 gcgcgcttca taggcttcca cttcacgatg cagcgtttgc tgttccacga gatcggtcac
    38461 ccgcgccagg cgggctctgc gttcggcaac catctcacgt agccgccgct caaaatcctg
    38521 atgctgaata cgtttacgct gccagcgggc aattttcggt ggcgcactgt cccactcgcg
    38581 caatgaccag gcggaggctt cacgcgcgat ggcttccagt tgtgccgcca gatgttccgc
    38641 cagccaggcc acttgcggca gctgttgctg ctcaactgca tgacgaagcg cagccagatt
    38701 atcgcccgcc tcatcgagac aagcctgcag tgttgtcgca cgggtttgaa aaaggtgtct
    38761 gtcaaaacgg gcacttagcg tggcaaattg cgctaccggg gcacaacgct gacgcagcgt
    38821 agcgagctgt ccttccagtt tttccagcag cagggcggtt ttcacgataa gacctcaatg
    38881 aaaatgataa ttgttatgct aaagtagcca ctctgtaagc tgacaacact caatatgcaa
    38941 cgaatcattt taatcatcat tggctggctg gcggtagtgc tgggtacgct gggcgtggta
    39001 ttaccggtat taccgacgac gccgtttatc ctgctggcgg cctggtgctt tgcccgttct
    39061 tccccgcgct ttcacgcctg gttgctgtac cgctcatggt ttggcagcta tctacgtttc
    39121 tggcagaaac atcatgcgat gccgcgcggc gtcaaaccgc gggcgatttt gcttattttg
    39181 ctcacgtttg ccatttctct gtggttcgtc cagatgccat gggtgcgcat catgttgctg
    39241 gtaattctcg cctgtttgct tttctatatg tggcgaattc cggtgattga tgaaaagcaa
    39301 gaaaagcact gaagcacaac aatcgcagtt gcaattattg cgtacagcca gtacattctg
    39361 gcgttttcga gcacaggcgc aggcggtcaa aggttaaaca actgttactt ttgatacgtt
    39421 taaaacgcgc cgtgagtacc accgtaacaa gcaggcatac acttatgacc gcgactgcac
    39481 agcagcttga gtatctcaaa aatagcatca aaagcattca ggactaccca aaacccggca
    39541 ttcttttccg cgatgtcacc agcttactgg aagacccgaa agcttacgct ctcagcatcg
    39601 acttgctggt tgagcgttac aaaaatgcgg gcattaccaa agttgtcggc accgaagcgc
    39661 gtggcttctt gtttggcgct ccggtagctc tgggtctggg cgttggcttt gtaccggtcc
    39721 gtaaaccggg caaactgccg cgtgaaacca tcagtgaaac ttacgacctg gaatacggca
    39781 ccgatcagct ggagatccac gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg
    39841 acgatctgct ggcaaccggc ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg
    39901 gtggtgaagt ggctgacgct gcgttcatta tcaacctgtt cgatctcggc ggcgaacagc
    39961 gtctcgaaaa acagggcatt accagctaca gccttgtccc gttcccgggc cattaattat
    40021 cgccagtctt gtgctgccca cgctacggac agcacaagat gtgcattcag cctcgccgtt
    40081 ctgacggggc tgtgttagca ttaccccttc gtgaatccac cttccagcgt ttcagagcct
    40141 gccaatgagt tatcaggtct tagcccgaaa atggcgccca caaacctttg ctgacgtcgt
    40201 cggccaggaa catgtgctga ccgcactggc gaacggcttg tcgttagggc gtattcatca
    40261 tgcttatctt ttttccggta cccgtggcgt cggaaaaacc tctatcgccc gactgctggc
    40321 gaaggggcta aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt gcgataactg
    40381 tcgtgaaatc gagcaggggc gctttgtcga tctgattgaa atcgacgccg cctcgcgcac
    40441 caaagttgaa gatacccgcg acctgctgga taacgtccag tacgctccgg cgcgtggtcg
    40501 tttcaaagtt tatctgatcg acgaagtgca tatgctgtcg cgccacagct ttaacgcact
    40561 gttaaaaacc cttgaagagc cgccggagca cgttaagttt ctgctggcga cgaccgatcc
    40621 acagaaattg ccggtgacga ttttgtcgcg ctgtttgcaa tttcatctca aggcgctgga
    40681 tgtcgagcaa attcgccatc aacttgagca catcctcaac gaagaacata tcgctcacga
    40741 gccgcgggcg ttgcaattgc tggcgcgcgc cgctgaaggc agcctgcgag atgccttaag
    40801 tctgaccgac caggcgattg ccagcggtga cggccaggtt tcaacccagg cagtcagtgc
    40861 gatgctgggt acgcttgacg acgatcaggc gctgtcgctg gttgaagcga tggtcgaggc
    40921 caacggcgag cgcgtaatgg cgctgattaa tgaagccgct gcccgtggta tcgagtggga
    40981 agcgttgctg gtggaaatgc tcggcctgtt gcatcgtatt gcgatggtac aactttcgcc
    41041 tgctgcactt ggcaacgaca tggccgccat tgagctgcgg atgcgtgaac tggcgcgcac
    41101 cataccgccg acggatattc agctttacta tcagacgctg ttgattggtc gcaaagaatt
    41161 accgtatgcg ccggaccgcc gcatgggcgt tgagatgacg ctgctgcgcg cgctggcgtt
    41221 ccatccacgt atgcctctgc ctgagccaga agtgccacgc cagtcctttg cgcccgtcgc
    41281 gccaacggca gtaatgacgc caacccaggt gccgccgcaa ccgcaatcag cgccgcagca
    41341 ggcaccgact gtaccgctcc cggaaaccac cagccaggtg ctggcggcgc gccagcagtt
    41401 gcagcgcgtg cagggagcaa ccaaagcaaa aaagagtgaa ccggcagccg ctacccgcgc
    41461 gcggccggtg aataacgctg cgctggaaag actggcttcg gtcaccgatc gcgttcaggc
    41521 gcgtccggtg ccatcggcgc tggaaaaagc gccagccaaa aaagaagcgt atcgctggaa
    41581 ggctaccact ccagtgatgc agcaaaaaga agtggtcgcc acgccgaagg cgctgaaaaa
    41641 agcgctggaa catgaaaaaa cgccggaact ggcggcgaag ctggcggcag aagccattga
    41701 gcgcgacgcg tgggcggcac aggtgagtca actttcgcta ccaaaactgg tcgaacaggt
    41761 ggccttaaat gcctggaaag aggagagcga caacgcagta tgtctgcatt tgcgctcctc
    41821 tcagcggcat ttgaacaacc gcggtgcaca gcaaaaactg gctgaagcgt tgagcacgtt
    41881 aaaaggttca acggttgaac tgactatcgt tgaagatgat aatcccgcgg tgcgtacgcc
    41941 gctggagtgg cgtcaggcga tatacgaaga aaaacttgcg caggcgcgcg agtccattat
    42001 tgcggataat aatattcaga ccctgcgtcg gttcttcgat gcggagctgg atgaagaaag
    42061 tatccgcccc atttgatcgt aagcacagct tacgttcgtc atccttaacg tgattgagag
    42121 agaaacctat gtttggtaaa ggcggtctgg gtaacctgat gaagcaagcc cagcagatgc
    42181 aagaaaaaat gcagaaaatg caggaagaga tcgcgcagct ggaagtcacc ggcgaatctg
    42241 gcgcaggtct ggtaaaagtg accatcaacg gtgcacacaa ctgccgtcgc gtagagatcg
    42301 acccgagcct gctggaagac gacaaagaga tgctggaaga cctggtggct gcagcattca
    42361 acgacgcagc acgtcgtatt gaagaaacgc agaaagaaaa aatggcctct gtatcctccg
    42421 gaatgcagct gccgcctggc tttaagatgc cgttctgatg caaaccagcc cgctgttaac
    42481 acagcttatg gaagcactgc gctgtctgcc gggcgttggc ccgaagtcgg cgcagcgtat
    42541 ggcgttcacg ctgcttcagc gcgatcgtag cggcgggatg cgtctggcgc aggcgctcac
    42601 ccgggcgatg tcggaaatcg gccactgcgc cgattgccgc actttcaccg aacaggaagt
    42661 ctgtaacatc tgttcgaatc cgcgtcgtca ggaaaacggt caaatctgtg tggtggagag
    42721 tccggcggac atctacgcca ttgagcagac ggggcagttt tcaggccgtt attttgtgtt
    42781 gatggggcac ctgtcaccgc tggacggcat cggtccggat gatatcggcc ttgatcgtct
    42841 ggaacagcgt ctggcagagg aaaaaatcac tgaagtgatc ctcgccacca atcccacggt
    42901 tgaaggtgaa gctaccgcta actacattgc cgagctttgc gcgcaatatg acgtggaagc
    42961 cagccgaatc gctcatggcg taccggttgg cggcgaactg gaaatggtcg atggcaccac
    43021 gctgtcacac tcccttgccg ggcgtcataa gattcgtttt taagcaaacg agagcaggat
    43081 cacctgctct cgcttgaaat tattctccct tgtccccatc tctccctcat cctgttttta
    43141 accttaaaat ggcattattg aggtagacct acatgaaagg acaagaaact cgtggttttc
    43201 agtcagaagt gaaacagctt ctgcacctga tgatccattc tctctattcc aataaagaaa
    43261 tcttcctgcg tgagcttatc tctaacgcct ccgatgcggc ggacaagctg cgtttccgtg
    43321 cgctatctaa cccggacctg tacgaaggtg atggcgaact gcgcgttcgt gtctctttcg
    43381 ataaagacaa gcgcacgctg accatctccg ataacggcgt ggggatgacc cgcgacgaag
    43441 tgattgacca tctggggact atcgctaaat ccggtaccaa atcattcctc gaatccctgg
    43501 gttctgacca ggcgaaagac agccagctga tcggtcagtt tggtgttggt ttctactctg
    43561 cgtttatcgt ggccgacaaa gtgaccgtgc gtactcgcgc ggcaggcgaa aaaccagaaa
    43621 acggcgtctt ctgggaatcg gctggcgaag gtgaatatac cgttgccgac atcaccaaag
    43681 aagatcgtgg tactgaaatc accctgcatc tgcgtgaagg cgaagacgag ttcctcgatg
    43741 actggcgcgt gcgttccatc atcagcaaat actccgacca tatcgcgctg ccggtagaga
    43801 tcgaaaaacg cgaagagaaa gacggcgaaa ccgttatctc ctgggagaaa atcaacaaag
    43861 cgcaggcgct gtggactcgt aacaagtcgg gaatcaccga tgaagagtac aaagagttct
    43921 acaaacacat cgcccacgac tttaatgatc cgctgacctg gagccacaac cgcgttgaag
    43981 gtaagcagga gtacaccagc ctgctgtaca tcccgtccca ggctccgtgg gatatgtgga
    44041 accgtgatca taaacacggc ctgaaactgt acgtccagcg tgtgttcatc atggacgacg
    44101 cagaacagtt catgccgaac tatctgcgct tcgtgcgtgg tctgattgac tccagcgatc
    44161 tgccgctgaa cgtttcccgt gaaatcctcc aggacagcac ggtaacgcgt aacctgcgca
    44221 atgcgctgac caagcgtgtg ctgcaaatgc tggaaaaact ggcgaaagac gacgcggaaa
    44281 aataccagac cttctggcaa cagtttggcc tggtactgaa agaaggtccg gcggaagatt
    44341 tcgctaacca ggaagcgatc gccaaactgc tgcgttttgc ttctacttat accgattctt
    44401 ctgcgcagac cgtgtctctg gaagactacg tttcccgcat gaaagaaggg caggagaaaa
    44461 tctactacat cactgcagac agctatgcgg cagcgaagag cagcccgcac ctggaactgc
    44521 tgcgtaagaa aggcatcgaa gttctgctgc tttccgaccg catcgatgag tggatgatga
    44581 actatctgac tgagttcgac ggtaaaccgt tccagtcggt gtctaaagtt gacgagtcgc
    44641 tggaaaaact ggctgacgaa gttgatgaga gcgcgaaaga agcggagaaa gcactgactc
    44701 cgttcatcga ccgtgtgaaa gccctgctcg gcgagcgcgt gaaagatgtc cgtctgactc
    44761 accgtctgac cgatacgcca gcgatcgttt cgaccgacgc ggacgaaatg agcactcaga
    44821 tggcgaaact gttcgctgcg gcgggccaga aagtgccaga agtgaaatac atcttcgaac
    44881 tgaacccgga tcacgtactg gtgaaacgtg cggcagatac tgaagatgaa gcgaagttca
    44941 gcgagtgggt agaactgctg ctggatcagg cgctgctggc agaacgcggc acgctggaag
    45001 atccgaacct gtttattcgt cgtatgaacc agctgctggt ttcctgatgt catgccggat
    45061 gacctttgtg tcatccggca tttttctttt catcatctgc actttccgca aattatctcg
    45121 ccattaaccg tttcagcctc aggtgccttt cttgaggcaa tcgcctgttg gtggtatcgt
    45181 ttatcgcttt ttcaaaaaat tcgacacatt ttaaggggat tttcgcaatg cgtatcattc
    45241 tgcttggcgc tccgggcgcg gggaaaggga ctcaggctca gttcatcatg gagaaatatg
    45301 gtattccgca aatctccact ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc
    45361 tgggtaaaca agcaaaagac attatggatg ctggcaaact ggtcaccgac gaactggtga
    45421 tcgcgctggt taaagagcgc attgctcagg aagactgccg taatggtttc ctgttggacg
    45481 gcttcccgcg taccattccg caggcagacg cgatgaaaga agcgggcatc aatgttgatt
    45541 acgttctgga attcgacgta ccggacgaac tgattgttga tcgtatcgta ggccgccgcg
    45601 ttcatgcgcc gtctggtcgt gtttatcacg ttaaattcaa tccgccgaaa gtagaaggca
    45661 aagacgacgt taccggtgaa gaactgacta cccgtaaaga cgatcaggaa gaaaccgtgc
    45721 gtaaacgtct ggttgaatac catcagatga ctgcaccgct gatcggctac tactccaaag
    45781 aagcggaagc gggtaacacc aaatacgcga aagttgacgg caccaagccg gttgctgaag
    45841 ttcgcgctga tctggaaaaa atcctcggct aattcgaaag cgcgcacgga cagtcccctc
    45901 gccccctcgg ggagagggtt agggtgaggg gaacaggccc gcacaagcaa acttatcagc
    45961 aatctcaggc cggatattca ttcggccttt tacaaaaacc tcaatctgcg ctattccttt
    46021 ttctgatttg acctctcaca gcaattagtt cttcttcctc acttttccgc tacaattatc
    46081 aacaagttga atcgataaga ggcggtaatg cgtcagacta aaaccggtat cctgctggca
    46141 aacctgggta cgcccgatgc ccccacacct gaagcggtaa aacgctatct gaaacaattt
    46201 ttaagcgaca gacgcgtggt tgatacctca cggttgttat ggtggccgtt gctgcgcggc
    46261 gtgattttgc cgctgcgctc gccgcgtgtg gcgaagctgt acgcctcggt ctggatggaa
    46321 gacggctcgc cgctgatggt ttacagccgc cagcaacagc aggcgttggc acaacgttta
    46381 ccggatacgc ccgtagcgct gggcatgagc tacggctcgc catcactgga aagcgccgtc
    46441 gatgaactcc tggcagagca tgtagatcat attgtggtgc tgccgcttta tccgcaattc
    46501 tcctgttcta cggtcggtgc ggtatgggat gaactggcac gcattctggc gcgcaaacgt
    46561 agcattccgg ggatatcgtt tattcgtgat tacgccgata accacgatta cattaatgca
    46621 ctggcgaaca gcgtacgcgc ttcttttgcc aaacatggcg aaccggatct gctgctgctc
    46681 tcttatcatg gcattcccca gcgttatgca gatgaaggcg atgattaccc gcaacgttgc
    46741 cgcacaacga ctcgcgaact ggcttccgca ctggggatgg caccggaaaa agtgatgatg
    46801 acctttcagt cgcgctttgg ccgggaaccc tggctgatgc cttataccga cgaaacgctg
    46861 aaaatgctcg gagaaaaagg cgtaggtcat atacaggtga tgtgcccggg ctttgctgcg
    46921 gattgtctgg agacgctgga agagattgcc gagcaaaacc gtgaggtctt cctcggtgcc
    46981 ggcgggaaaa aatatgaata tattccagcg cttaatgcca cgccggaaca tatcgaaatg
    47041 atggctaatc ttgttgccgc gtatcgctaa agctgagcgg taaagaactg agcgccgtcg
    47101 cgaagagcct cgtcggcggt tttcatcatt cgtgaataat gcaaaaaggc atgcagcgta
    47161 cctgggtaga gtttgaactc acagggctgc tgatgcgccg ctaacgtctg gtaaagcaga
    47221 cggctgtcat ccagcagcgg atcgaactcc gccccggcaa taaaacaggg cggaacttcg
    47281 cgagtgagat cattattaaa cagacagtaa tacggtgact cgcggtccgc gtcgttgctt
    47341 aaatacgcct cttcatacat ctgcaaatcc tgttgcgtta agccatccca gacaccgccc
    47401 aacagacgac gagtcacgga atcccgtaat ccgtaaagcc cataccacag caaaacaccc
    47461 gcaattttac cgcaatcgat ctgtttatca cgcaaccaca acgcactggc gagcgccagc
    47521 atggcacctg cggaatcacc ggcaaagcca atgcgggaca tattgatttg ataatcctcc
    47581 gcctgctggt ggaaataaca acaagcagcc acaatttcct ctatcgcttg cggaaaacgc
    47641 gcttcaggtg aaagggggta atcaatacca atcaccgtac attggctgta gcttgccagc
    47701 aggcgcatga tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc tccatgcaaa
    47761 taaaatagcg tcgccgggct atctggctgc ggacaaaaga gacgtgtttc cacctgccca
    47821 tattttgttg gaaccatgta agctctggtt gccatttctg gagcgcccgc attccagaat
    47881 cggcgctcaa gcgtgtaata ctgtcgttgc tcagcaatcg ttcccgttgc gggccaggac
    47941 ggtaaatccg actgaagagt attcacaacg gtcttcattt cagcagaaat aaggtccaga
    48001 acaggtagtt tgttttccgg cttcatacaa aaactccttt caaattacgt cattgtaagg
    48061 aaccactgcc atgaaaatgc gatcccgcct gctgatattg aaactggctg cgtctcgcgc
    48121 gctcccgtca gattgtgtta acattcgccg ctcagttaac cacccgtaaa aacaaccatg
    48181 aaatttcccg gtaaacgtaa atccaaacat tacttccccg taaatgcacg cgatccgctg
    48241 cttcagcaat tccagccaga aaacgaaacc agcgctgcct gggtagtggg tatcgatcaa
    48301 acgctggtcg atattgaagc gaaagtggat gatgaattca ttgagcgtta tggattaagc
    48361 gccgggcatt cactggtgat tgaggatgat gtagccgaag cgctttatca ggaactaaaa
    48421 cagaaaaacc tgattaccca tcagtttgcg ggtggcacca ttggtaacac catgcataac
    48481 tactcggtgc tcgcggacga ccgttcggtg ctgctgggcg tcatgtgcag caatattgaa
    48541 attggcagct atgcctatcg ttacctgtgt aacacctcca gccgtaccga tcttaactat
    48601 ctacaaggcg tggatggccc gattggtcgt tgctttacgc tgattggcga gtccggggaa
    48661 cgtacctttg ctatcagccc tggccacatg aaccagctgc gggctgaaag tattccggaa
    48721 gatgtgattg ccggagcctc ggctctggtt ctcacctctt atctggtgcg ttgcaagccg
    48781 ggtgaaccca tgccggaagc aaccatgaaa gccattgagt acgcgaagaa atataacgta
    48841 ccggtggtgc tgacgctggg aactaagttt gtcattgccg agaatccgca gtggtggcag
    48901 caattcctca aagaccacgt ctctatcctt gcgatgaacg aagatgaagc cgaagcgttg
    48961 accggagaaa gcgatccgtt gttggcatct gacaaggcgc tggactgggt agatctggtg
    49021 ctgtgcaccg ccgggccaat cggcttgtat atggcgggct ttaccgaaga cgaagcgaaa
    49081 cgtaaaaccc agcatccgct gctgccgggc gctatagcgg aattcaacca gtatgagttt
    49141 agccgcgcca tgcgccacaa ggattgccag aatccgctgc gtgtatattc gcacattgcg
    49201 ccgtacatgg gcgggccgga aaaaatcatg aacactaatg gagcggggga tggcgcattg
    49261 gcagcgttgc tgcatgacat taccgccaac agctaccatc gtagcaacgt accaaactcc
    49321 agcaaacata aattcacctg gttaacttat tcatcgttag cgcaggtgtg taaatatgct
    49381 aaccgtgtga gctatcaggt actgaaccag cattcacctc gtttaacgcg cggcttgccg
    49441 gagcgtgaag acagcctgga agagtcttac tgggatcgtt aagttatcgt cggttcgtag
    49501 gccagataag gcgttcacgc cgcatctggc atttggctct cgatgcctga tgcgacgctg
    49561 gcgcgtctta tcatgcctac atatttttca tattttacat ccggcaacca ccgtttaccc
    49621 cgtcaccacc tcacccgccg gtggcgtttc cagcagttcc agcatggtac gggcgatttc
    49681 acgctcgccc atcactacct gattcgcacc acgttcggtg atatacgcca cttcatcgtc
    49741 ataatgggcg cgggcaataa tctcaatatc cggatttttc gcgcgggcag atgccacaat
    49801 ctcacccgct tcataaccgt tgggaatcgt caggatcagc cattttgcac attccagatg
    49861 cgccagttgc ataatttctt cgttcgccgc attgcccaat actgcgcgga ccccgcgctc
    49921 tcgcagctca tcaacacggg ttcgtgacgt ctcaatcacc accagcggaa tatcagaggc
    49981 gagcaatttc tcccccagca ggctgcctac acgaccgtaa cccaccagta gcgcatggtt
    50041 gcaaatatcc actgggatct gcttctcttc ttcgattgcc tcttccagcg tctgctcttc
    50101 cagcgtttcg gtcttcgcca gatatttctc cagtagtgcg aacagtaccg ggttgagcat
    50161 aatcgacagg atcgcccctg ccagtaccag gttttgtccg gcctgcggca gtaaattcaa
    50221 tgccattccc agtcccgcca ggataaacgc gaactcacca atctgcgcca ggctggcggc
    50281 gatggttaat gccgtacgtt gggagtgacc aaacagtcgc accaggaaaa atgcggctaa
    50341 cgacttacca aacagaataa tcgccagcgt cgccagcact gccagcggtt gctgaatcag
    50401 aattaacgga tcaaacaaca tcccgacgga gacaaaaaac agcaccgcaa acgcgtcgcg
    50461 caatggcagc gtatcgtggg cggcacggtg actcagttca gactcgttca gtaccatccc
    50521 ggcaaagaac gcaccgagtg caaaggagac atcaaacagc tctaccgcac caaaggcaat
    50581 ccctaacgcc agcgccagca ccgacagggt aaacagctcg cgagaaccgg ttgccgcgct
    50641 gcgtgccata atccacggca ccagacggcg acctaccagc atcataatgg cgataaatgc
    50701 gatcactttg ccgatggtga tccccatatc gactgcaaga gtggcaaagc ccacatcgcc
    50761 ctgttccatc attcctgcca ctgcgggcag caacaccagc gtcagaacca ttaccaggtc
    50821 ttccacaatc aaccaaccga tggcgatttg cccacgctga ctgtcaatta attgccgttc
    50881 ttcaagtgcg cgcagtaaca ccacggtact ggcggtggaa agacataaac cgaacacgat
    50941 accggtcatt aacgaccagc ccagcacggc agagagcgcc atacccagca gcgtcgccac
    51001 ggctatctgg gcgatcgcac cgggaatggc gatggccttt accgccatca aatccttcag
    51061 cgaaaagtgc aaaccgacgc caaacatcaa cagaatgacg cccagttcag ccagttccgg
    51121 cgcaagcttg gtatcggcaa caaagcccgg agtgaatggt cctgccagca cacccgctaa
    51181 cagatatccc accagaggag aaatacgtag tttattggcc agcatgccga ggataaaggc
    51241 gagcacaagg ccgccaacaa tggtggtgat aagcggggtg gcgtgatgca ttccgtctcc
    51301 ttttcctggt ggttattgtc catttttggc cgggaaaacc aaaattacag gtaatagttt
    51361 atgacaattt cattgatgat gttcatgaat aattattgaa ttttgcagaa aaatggaatt
    51421 agctgcaaaa aaagcacgga tcggaaaaca gaaggcggtt taacagagga aaggcttatg
    51481 gcgactgtaa aacgatgcag ccaaagtttg cctttggctg caatgaaatc agtctttatg
    51541 ccggttatca ggcaggaata tggtcaacat ccccaatagt ggcaggaaag cacagatttt
    51601 atagactaac tcgatgctgg tgtgatcggc gataagcccc agaactgccg ctcccagacc
    51661 tcccatgcca aaagcaaaac cgaaaaagag tccagaaacc ataccgatac gtcccggaag
    51721 tagctcctga gcgtagacca gaatggcaga gaatgccgaa gcgaggataa atccaataat
    51781 caccgttaaa acccccgtcc agtgcaggct ggcgtagggt aaaatcagcg taaacggcgc
    51841 aacgccgagg atagagcccc aaatcacata tttccgtcca attttatccc ctacaggccc
    51901 gccgatcacc gtacctgccg caacggcaaa caggaaggca aacagatgaa gctgggcatt
    51961 ctggatagat aatccgaatt tttgcatcag ataaaaggtg taatagctgc tgatgctcgc
    52021 catatagaaa tatttcgaga aaatgaggat taacagaatg ctgactgcca gtaccacttt
    52081 gttgcgcggc agtggattga ttatcgtcgc tttggatttt cctttattca ttcggtgctg
    52141 tgccgagtac caacggctaa tttgcgccaa caccacgatc gccagcagtg ccgcaagcac
    52201 aaaccaggca acgttgcctt tgccataagg cgcgataatc accgccgcca gcaagggtcc
    52261 cagggaactg ccaaagttgc cgccgacctg aaagatagat tgcgccaggc catgccgccc
    52321 gccggaagcc atacgggcta cgcgagaaga ttccggatga aagaccgatg aaccggtacc
    52381 gaccagcgcc gccgccagca gaactgcgcc aaaactgccc gccagcgcaa gcagcaccag
    52441 accacttaag gtaaagcaca tgccaattgg caacgaccac ggcatcggat atttatcggt
    52501 ccagtagccg accactggtt gcagtagcga agaggcgagc tggaaggtga gggttatcat
    52561 gccaatctgc ataaatgtca gagaaaattc tgactgaagc agcggataaa tcgccagaat
    52621 cagcgattgg atcatgtcgt tcagcagatg tgagaggctg atagcaccta aaataccaaa
    52681 cgatgttcgg gccttggtcg ttgacgcagc cgcgcccgcc acaggctggg gttgttcact
    52741 cattgccata ggaaagtcac tttttcaggg ttgcgatgta aagaatgatc ttatttgtga
    52801 ttattacccg actaacatac ctgtatgcgt cgcctgaagg aagtctcaac gccgaataca
    52861 gaatttctaa tctggatgca gatttatctt caccggacgc agacttgtct atgatgtcgc
    52921 gtcatactat ttttcaacac gttgaaatca ggtcagggag agaagtatga aattattgca
    52981 gcggggcgtg gcgttagcgc tgttaaccac atttacactg gcgagtgaaa ctgctctggc
    53041 gtatgagcag gataaaacct acaaaattac agttctgcat accaatgatc atcatgggca
    53101 tttttggcgc aatgaatatg gcgaatatgg tctggcggcg caaaaaacgc tggtggatgg
    53161 tatccgcaaa gaggttgcgg ctgaaggcgg tagcgtgctg ctactttccg gtggcgacat
    53221 taacactggc gtgcccgagt ctgacttaca ggatgccgaa cctgattttc gcggtatgaa
    53281 tctggtgggc tatgacgcga tggcgatcgg taatcatgaa tttgataatc cgctcaccgt
    53341 attacgccag caggaaaagt gggccaagtt cccgttgctt tccgcgaata tctaccagaa
    53401 aagtactggc gagcgcctgt ttaaaccgtg ggcgctgttt aagcgtcagg atctgaaaat
    53461 tgccgtcatt gggctgacaa ccgatgacac agcaaaaatt ggtaacccgg aatacttcac
    53521 tgatgtcgaa tttcgtaagc ccgccgatga agcgaagctg gtgattcagg agctgcaaca
    53581 gacagaaaag ccagacatta ttatcgcggc gacccatatg gggcattacg ataatggtga
    53641 gcacggctct aacgcaccgg gcgatgtgga gatggcacgc gcgctgcctg ccggatcgct
    53701 ggcgatgatc gtcggtggtc actcgcaaga tccggtctgc atggcggcag aaaacaaaaa
    53761 acaggtcgat tacgtgccgg gtacgccatg caagccagat caacaaaacg gcatctggat
    53821 tgtgcaggcg catgagtggg gcaaatacgt gggacgggct gattttgagt ttcgtaatgg
    53881 cgaaatgaaa atggttaact accagctgat tccggtgaac ctgaagaaga aagtgacctg
    53941 ggaagacggg aaaagcgagc gcgtgcttta cactcctgaa atcgctgaaa accagcaaat
    54001 gatctcgctg ttatcaccgt tccagaacaa aggcaaagcg cagctggaag tgaaaatagg
    54061 cgaaaccaat ggtcgtctgg aaggcgatcg tgacaaagtg cgttttgtac agactaatat
    54121 ggggcggttg attctggcag cccaaatgga tcgcactggt gccgactttg cggtgatgag
    54181 cggaggcgga attcgtgatt ctatcgaagc aggcgatatc agctataaaa acgtgctgaa
    54241 agtgcagcca ttcggcaatg tggtggtgta tgccgacatg accggtaaag aggtgattga
    54301 ttacctgacc gccgtcgcgc agatgaagcc agattcaggt gcctacccgc aatttgccaa
    54361 cgttagcttt gtggcgaaag acggcaaact gaacgacctt aaaatcaaag gcgaaccggt
    54421 cgatccggcg aaaacttacc gtatggcgac attaaacttc aatgccaccg gcggtgatgg
    54481 atatccgcgc cttgataaca aaccgggcta tgtgaatacc ggctttattg atgccgaagt
    54541 gctgaaagcg tatatccaga aaagctcgcc gctggatgtg agtgtttatg aaccgaaagg
    54601 tgaggtgagc tggcagtaat ccgaaagtgc cggatgtttg catccggcac aatgcttaat
    54661 cgcggcgggc gatatcagca aatttggcat cgaggatctt tgccagatcg cctgccgcca
    54721 gttcgatatc cagtccgcgc ttgccgccgg aaacataaat agtggcaaat tcttgtgcgg
    54781 gggcgtcgat aatcgttggc agacgttttt tctgccccag tgggctaatc cccccaacca
    54841 ggtatcccgt cgaacgctgc gcgaccatcg gatcggccat ctcaactttc ttggcaccca
    54901 gcgcttttgc tactttttta agatccagtt gacctgcaac cggcgtaacg gccactgcca
    54961 gatgtttcat atcaccgttc actgccacca gcagcgtttt gtagacctga tccggattca
    55021 aacctaattt tttgacgact tcatcgccaa aattggtttc agccggatcg tgctcgtagg
    55081 tatggatttg aaacgaaatc ttgttttttt cgagtaattt aactgcgggt gtcatggtaa
    55141 ttttcctgcc ttaaacaaaa aaacgaagca agcatacgcc tgatggcggt ccaaaaaaaa
    55201 gagtcatctt gcctaagagt attggcagga tggtgagatt gagcgacaat cgagttacac
    55261 cgtcgcttaa agtgacggca taataataaa aaaatgaaat tcctctttga cgggccaata
    55321 gcgatattgg ccattttttt agcgcaacat ttgcggcaaa ttcccttctc catacaggtg
    55381 tagtgcaccg accgcgacca catatcgccc cggcggcatg gcgcgtaatt tatcccgcca
    55441 ggcgagattt cgctgatgca tcagcacatc gtacagcgac tgactgaacg tattgggcag
    55501 cgttatttca ttattttgcg gcggcgcatt cagccaccag ctcatcattt gttgcagcaa
    55561 ccgtgcgttg gtatgccagt gggtcagcgt atcgtccagc agcgccagtc ctttgtcagg
    55621 gagctggagc aacatggtaa tctggttttc agccccttcc agttcaatca cgggtttatg
    55681 ttgttgcttc gccgcctgca ataactggta atcgataccg tattctgccc gcagccccag
    55741 tttttgtgcc tgcgtcgcct gaagaaccat cgcgatttgc cacagcggtt gagtagaaaa
    55801 gagcgacgga gaaatgccca tctcctggct aatgtgctgc agattttgga gttgctcctc
    55861 gttaatgcgc tcttccagcg cctcgcaagt aggcaaatta gcaaaaggcg tatcgctggt
    55921 ggaaacatcc gcctcgacga tcagcgcatc ggcgtttttg agctttttga gcaaacgggt
    55981 gggcagggga gccatatcgt ggctacccat atgaatactg ccaatcagat gaaaatggcg
    56041 attgccggga agggtgatat cgatggcagg ccaggtgtaa tgattaccgc gcagcgcggc
    56101 ccaaagcgtt tttacccggt acaacagatc catacgacct ccctttgtga aatatcatgc
    56161 tagcgcgcgg tgagggatgg cgcaaatgga gttggggact tgtttaatgt gtttgtatga
    56221 ttcagtatgt tcttgcatcg ctattcacag ggaagcaaca gttaaaaacc atgaaacagg
    56281 caacaagaaa accgacgaca cccggagata ttcttctcta tgaatatctg gaaccgctcg
    56341 atttgaaaat caatgagtta gcagagttgc tgcatgttca tcgtaatagc gtcagtgcac
    56401 tgatcaataa caatcgtaaa ctcaccactg agatggcatt ccgtctggcg aaagtttttg
    56461 ataccacagt cgattttagg ctaaacctcc aggcggcagt tgatttttgg gaagttgaaa
    56521 acaacatgcg cacccaggaa gaattgggac ggattgaaac agtggctgaa tatttggcac
    56581 gccgtgaaga gcgtgcaaaa aaggtcgcgt aaggccaaaa catcgcttca tctcgtaggc
    56641 cggataaggc gtttacgccg catccggcag tcatgcgtcg atgccaaatg cgccgcccta
    56701 aagcagcgca tccgcaatga cgtacttatt ccttcggttt aaaccgcagc aaccggttgg
    56761 cgttactcac tacggtaatc gacgagagcg ccattgccgc tccggcaact accgggttaa
    56821 gcagtgttcc agtaaacggc cacaaaatac cggcggcgac cggaataccg atgctgttgt
    56881 agataaaggc accgagcagg ttctgcttca tgttgcgcag cgttgcgcgg gaaatcgcga
    56941 gcgcatccgc aacgcccatc aggctatggc gcatcagggt aatcgccgcg gtttcaatgg
    57001 caacatcact gccgccaccc atcgcaatgc cgacatccgc ctgggccagc gctggcgcgt
    57061 cgttaatgcc gtcgcccacc attgccacct gacgtccttc actttgcaga cgtttgatcg
    57121 cttcggcttt accgtccggc agcactccgg caatcacttc atcaattcca gcttctttgg
    57181 cgatcgcatt ggcggtggtt gggttatccc cggtcaacat caccagacga tatcccgctt
    57241 tatgcaggcg ttgcagcgcc gccacgctat cactacgcaa cggatcgcgt acagccagca
    57301 gggctactgc tttcccgtca atcgccagca gcacaggcgt tgctccttgc gatgcctgag
    57361 cagtaatctc cgcttcgata gctttggtac caacctgttg ctcatttaac agcgcctgat
    57421 tgcccagcaa taacgcatga ccttcagctt caccgctcac gcccagcccg cgcaatgtgc
    57481 ggaaaccgtt gacctgcggc agctgcatat cacctgcttt atcgaggatc gcccgtgcca
    57541 gcggatggct ggaaccttgc tccagtgccg ccgccagacg caatgcctgc gcttcatcaa
    57601 catcagcaaa cgttttcact gcgacaacct gcggcttccc ttcggtcagc gtcccggttt
    57661 tatcgaacac gacggtatcg agtgtactgg cgcgttgcag cgcgtcggcg tcccgcacca
    57721 gcacgccaaa ctcagccgcc cgcccgacgc cggaaataat cgacatcggc gtcgccagcc
    57781 ccagcgcaca cggacaggcg ataatcagta ccgtggtggc aatcaccagg gtataaacaa
    57841 tttgcggtgc cggaccaaag aagtaccaga ttgccgcact gaccagcgca atcaccacca
    57901 ccacaggtac aaatacggct gagattttat ccgccagctg accgatttct ggcttgctgc
    57961 tctgggcctg gcgcaccatg cgaatgatcc gtgacagcgt ggtatggctg ccaaccgcac
    58021 tggcacgaaa cagcacactg ccgtcctgta ccactgtccc ggcatggacg ctttcgcctt
    58081 cgcctttttg ctgtgggatt ggttcgcccg tcagcatcgc ttcatccagc catgcttcgc
    58141 cctgggtaat ctcgccatct accggcacgc gatcgccggt cgtcaggcgc agtaacatcc
    58201 ctggctgaac ttccgccagc ggcacgcttt tttcaccctc tgcggtgacc actcgcgccg
    58261 tcggtggggt taaatcgagt aatttctcca gcgccttaga agaacgctga cgtgcgcgcg
    58321 cttccagcat atggccgaga ttaatcagac cgataatcat cgcactggct tcatagtaaa
    58381 gatgccgcgc ttccatcggg aaccactgcg gccacaggtt gacgctcatc gaatagagcc
    58441 acgccacgcc agtacccagc gccaccagcg tatccatcgt cgctgcaccg ttcagcaggc
    58501 ttttccatgc actgcggtaa aaatggccgc cggcgaaaac catcaccgcc agggttatca
    58561 ggccgataac caaccacagg ctgcggttgt cagcggtgac catcatgtta tcgccaatca
    58621 tcccccagac catcaccggg atacccaccg ccagtgcgac aattgcctgc cagcggaagc
    58681 gcttcatcgt agcaacggcg gtttcctgct ggcgctcacg gcgtttcgcg tcatcttcaa
    58741 tcgcttccgc gccgtagccc gctttttcca ccgcctgcac taaatcttgt ggggaggcac
    58801 tgcccatcac cagcgcagta cgctccgcca ggtttacccg tgcctgagtg acgcccggta
    58861 cgctttgcag cgcattttgt acgcgggtga cacagctggc gcagctcatg ccgctcagca
    58921 gcaactgctg gctgtcatcg tcatcggcgg tcgctgccgg aagcgcctca gaaaccgctg
    58981 tcagtgcttc cgacgggatt gatgactccg ccagcggttt agcctttggg tggcttacag
    59041 atgcgtcata acccgcttgt ttgatggttt cgatcagctg ttctgcactg gcagtcccgg
    59101 taacgtgcgc ttcagtgata gacacatccg cttgttccac atccgggcgc tgttcgagac
    59161 tttctttcac gcgtttaacg cagtgaccgc aggacaggcc gtccagggtc aggtcgataa
    59221 tttgtgacat aaaacactcc tttaagacag ttttgactgg ctgtgataaa ggttaaacct
    59281 tccagcaagg ggaaggtcaa gaaattaata aaccaggcga gtaaaagtcc gtaaagatta
    59341 aaaaattggc tcaatttgca tcaggattag acatttatct ctttgttttc ctgtagttaa
    59401 gttgcgggtg ctaagttaaa tcaggatgcc tgaaaatcgg caccggggtg aggaattacc
    59461 tcccgcatct ataaaaagga gttaacaaaa gatgttagat gcaaacaaat tacagcaggc
    59521 agtggatcag gcttacaccc aatttcactc acttaacggc ggtcaaaatg ccgactacat
    59581 tccctttctg gcgaatgtgc ctggtcaact ggcggcagtg gctatcgtga cctgcgatgg
    59641 caaggtctat agtgcgggtg acagtgatta tcgctttgcg ctggaatcca tctcgaaagt
    59701 ctgtacgtta gcccttgcgt tagaggatgt cggcccgcag gcggtacagg acaaaattgg
    59761 cgctgacccg accggattgc cctttaactc agttatcgcc ttagagttgc atggcggcaa
    59821 accgctttcg ccactggtaa atgctggcgc cattgccacc accagcctga ttaacgctga
    59881 aaatgttgaa caacgctggc agcgaatttt acatatccaa cagcaactgg ctggtgagca
    59941 ggtagcgctc tctgacgaag tcaaccagtc ggaacaaaca accaacttcc ataaccgggc
    60001 cattgcctgg ctgctgtact ccgccggata tctctattgt gatgcaatgg aagcctgtga
    60061 cgtgtacacc cgtcagtgct ccacgctcct caatactgtt gaactggcaa cacttggcgc
    60121 gacgctggcg gcggggggag tgaatccgct gacgcataaa cgcgttcttc aggccgataa
    60181 cgtgccgtac attctggccg aaatgatgat ggaagggctg tatggtcgct ccggtgactg
    60241 ggcatatcgc gttggtttac cgggcaaaag cggtgtaggt ggcggtattc tggcggtcgt
    60301 ccctggtgtg atgggaattg ccgcgttctc accaccgctg gacgaagaag gcaacagtgt
    60361 tcgcggtcaa aaaatggtgg catcggtcgc taagcaactc ggctataacg tgtttaaggg
    60421 ctgatcatga tgaacacgga aggtaataac ggtaacaaac ccctcggtct atggaacgtc
    60481 gtttccatcg gcattggggc aatggtgggg gcggggatct tcgcgctgct ggggcaggct
    60541 gcgttgctga tggaagcctc gacctgggtc gcctttgctt ttggcggtat tgtggcgatg
    60601 ttttccggtt atgcctatgc gcgtctgggg gcgagctatc ccagcaatgg cggcattatc
    60661 gacttctttc gtcgcggatt aggcaacggc gtcttttcgc tggcgctctc gttactgtac
    60721 ctgttgacgc tggcggtgag catcgccatg gtcgcccgtg cttttggcgc ttatgccgtg
    60781 cagtttttgc atgaaggcag ccaggaggag caccttattt tgctctacgc gttggggatc
    60841 attgcggtga tgacgctttt caactcctta agcaaccatg cggtagggcg gctggaagtg
    60901 atcctcgtcg gcattaaaat gatgatcctg ttattgctga ttattgccgg tgtctggtcg
    60961 ctgcaaccgg cgcatatttc cgtctctgcg ccccccagct ccggtgcgtt cttctcctgt
    61021 atcgggataa ccttccttgc ctatgcgggc tttggcatga tggcgaacgc ggcggataaa
    61081 gtgaaagatc cgcaggtcat tatgccgcgg gcgtttctgg tggcgattgg cgttaccacg
    61141 ttgctttata tctcgctggc actggttttg cttagcgatg tatcggcatt agagttagaa
    61201 aaatatgccg ataccgccgt agcgcaggct gcttctccgc tgctcggaca tgtgggttat
    61261 gtgatcgtcg tcatcggcgc tttactggcg acggcttcag ccattaacgc gaacctgttc
    61321 gccgtgttta acatcatgga caacatgggc agcgaacgcg aactgccgaa gctaatgaat
    61381 aaatccctgt ggcggcagag tacctggggc aacatcattg tcgtggtgtt gattatgctg
    61441 atgacggcgg cgctaaattt aggctcactc gccagtgttg ccagcgccac ctttttgatt
    61501 tgctacctgg cggtgtttgt ggtggcgatc cgcctgcgtc atgatattca cgcctcgttg
    61561 ccgattctta tcgttggtac gttggtgatg ttgttggtga tcgttggctt tatctacagt
    61621 ctgtggtccc agggtagccg tgcgttgata tggattattg gctcactctt actcagcctt
    61681 attgtggcaa tggtcatgaa gcgcaataaa accgtataac atctctctgt gcgcagtact
    61741 tcctgtatta ttgtggtggc ggtcgatatt cgcactggca aaaaaacgtg cttgaatatc
    61801 tgttgaaacc ctttaacaaa gcacaggagg cgttgcgcga acgatgaaca tcagcgatgt
    61861 agcaaaaatt accggcctga ctagcaaagc cattcgcttc tatgaagaga aggggctggt
    61921 gacgccgccg atgcgcagcg aaaacggtta tcgcacctac acgcagcagc atctcaacga
    61981 actgacctta ctgcgccagg cacggcaggt gggctttaac ctggaagaga gcggcgagct
    62041 ggtgaatctg tttaacgacc cgcagcggca cagcgccgac gtcaaacggc gcacgctgga
    62101 gaaggtggcg gagatcgaac gacacattga ggagctgcaa tccatgcgcg accagctgct
    62161 ggcactggcg aatgcctgcc ctggcgatga cagcgccgac tgcccgatta tcgaaaatct
    62221 ctccggctgc tgtcatcatc gggcagggtg attaagacga gacggctcgg atatgtaggg
    62281 ttatcccttc tatcgcaatg acttcaacat gcgtacctgc accgagatcc tcgctggcgc
    62341 tgacaggcca tgaactgtca ccgacgcgca tatgaccgcg cccgttgacc agcggagatt
    62401 ccagcacaaa acgtcggcca atcagctgct gcccgcgctg gtttaaatga ctatcgctgt
    62461 gcttttgttc gcgcacccgc cgcgacaacc atttccacca cagccaggcg gcgagcagcg
    62521 tcaggatggc aaacattacc ccttgccact cccaacccag cggcaccagc cagaccacca
    62581 ggccggtaat caccgctgcc acgccgctcc acaacaaata accatttccg cccagcatct
    62641 cggctgccag cagcaaaccg ccgagactga gccagaaaat atgtggatga acgaccatta
    62701 actccatcat ggctgagtcc gtttgttggc gctgtctttc accagctcgg caatcccggc
    62761 aatcgacccc atcaggctgc tggcctctaa tggcatcatc accactttgc tgttactgga
    62821 cgaaccgatc tgctgtaacg cttcggtgta tttctgcgct acgaagtagt tcaccgcctg
    62881 aatatcacct gaggcgatgg cttcagacac cattttggtg gcgcgggctt ctgcttcagc
    62941 ggaacgttca cgcgcttcag cctgtaaaaa cgccgactga cgttcgcctt ccgctttcag
    63001 gatttgcgac tgtttttcac cttcggcttt gaggatttcc gcctgacgga tcccttccgc
    63061 ttcaaggatg taagcgcgtt tggtacgttc cgctttcatc tgcgcgttca ttgaagagat
    63121 aagctctgcc ggtgggcgca cgtcgcgaat ttcaatacgg gtgactttaa tcccccacgg
    63181 gttggtggct tcatcgacaa tacgcagcag gcgtgagttg atgctgtcgc gctgagagag
    63241 catttcatca agttccattg aacccagcac ggtacggatg ttagtcatgg tcaggttgat
    63301 gatagccagc tccagattgc tgacctcata agccgcgcgt ggcgcgtcaa tcacctgaat
    63361 aaagcacacg gcgtcgatgg taacgttggc gttatctttc gagataactt cctgggaagg
    63421 gatatcgagc acttgctcca tcatattgat cttgcgacca atgcgatcca taaacggcac
    63481 caccagactg agccccggct gtaacgtttt ggtatagcga ccaaaacgtt ctaccgtcca
    63541 ttgatagccc tgcggcacga ttttgacgcc cgcgccgaca atgaccagcg cgacaaaaat
    63601 gagaatcggg ataaagataa gcatcggaaa aacctcctgt tgtaccgtcc ataatcagca
    63661 aaattgctgc ttgattaaac aaattatacc tgattactga aagagagttc ccccttattc
    63721 ctgcgaagga taaactgttt ttagtaaaaa tcagaaaaag ggaacagcga tgcaggaaaa
    63781 tagtcctttg cttcagctac aaaacgtagg atatctggcg ggtgatacga agattcttaa
    63841 taacatcaat ttttcgctgc gtgctggcga atttaagtta attaccggtc cttctggttg
    63901 tggcaaaagt acgctgctaa aaatagttgc ttcattgatc agcccaacca gcggaacgtt
    63961 actgtttgaa ggggaggatg tcagcacact aaagccagaa atctaccgcc agcaagtctc
    64021 ttactgcgcc cagacaccga cgctgtttgg cgatacggta tacgataatc tgatctttcc
    64081 ctggcagatc cgtaaccagc agcctgaccc ggccattttt ctcgattttc tcgaacgctt
    64141 cgctttgccg gacagcattt tgacgaagga tatcgccgag ctatctggtg gtgaaaaaca
    64201 acgcatctca ttgattcgta acctgcaatt tatgccgaag gttttattgc tggatgaaat
    64261 aaccagtgcg ctggatgaaa gtaataaaca taacgtcaat gagatgatcc atcgttatgt
    64321 gcgcgagcaa aatattgccg tgctgtgggt gacacacgat aaagacgaaa ttaatcatgc
    64381 ggataaagtg attacactgc aaccgcatgc cggagaaatg caggaagcac gctatgaact
    64441 cgcataatat tactaacgaa tcattagcac tggcattaat gctggtggtg gtggcaatct
    64501 taattagcca taaagaaaaa ctggcgctgg agaaagatat tctctggagc gtcgggcgag
    64561 cgataattca gctgattatt gtcggctatg tgctgaagta tattttcagc gtggatgatg
    64621 ccagcctgac attattgatg gtgttattta tctgctttaa tgcggcgtgg aacgcgcaaa
    64681 aacgcagtaa atatattgct aaagctttta tctcatcgtt tgttgccatt acggtcgggg
    64741 cgggaattac cctggcggtg ctgattctct cagggtcgat tgaatttatc ccgatgcagg
    64801 tgatccctat cgccgggatg attgccggta acgccatggt agcggtgggg ttgtgttaca
    64861 acaatttagg gcaacgggtc attagcgaac agcaacagat tcaggagaaa ctgagtcttg
    64921 gtgcgacgcc gaagcaggct tcagcgatat tgattcgcga cagtattcgc gcggctttaa
    64981 ttccgacggt cgattcagca aaaacggttg gcttagtgag tttaccagga atgatgtccg
    65041 ggctgatatt tgccgggatt gatccggtga aggcgattaa atatcagatt atggtgacct
    65101 ttatgctgct ctcaaccgcc agcttgtcga ccattattgc ctgctattta acctatcgta
    65161 agttttataa ttcgcgccac cagttggtgg tgacgcaatt gaagaagaaa taatgcgatg
    65221 ccggatgcgc agcatcgcat ccggcgttgt ggttcatgtg ccgatcaacg gaatatcaat
    65281 acaacaatgc atacagctgg cggcgatact tcgacgccag tgcatcaccc gtacccagcg
    65341 cagcgaggat ctcctggaac gttttacgcg tctgaccgtc tgcggcggtg agatctttac
    65401 gcagatgccc gaacagcaac tccagcgcct cttcattgcg cccaacctgg tgcagttgca
    65461 gagccagttg cgtcgccagt gcggcatctt ctggattctc cgccacctgc tgttgcaact
    65521 gttgaatttc cggcgtatca gccgcctgct tcagcagttc gatttgcgcc accagcccct
    65581 ggtagcgggt gtcctgatcc tgcaacggaa tggttttcag caccgcttcc gcatcttcag
    65641 aacggttcag cgcaatcagc gtttctgcca gcagcaggcc gatctccccg ttctgattcg
    65701 acaactgcca ggcgtctttc agcaatggca gggcatcggt gtaattgcct tcctgcatca
    65761 gttgcatcgc ctgctgcgct ttcagctctt cttcgcgcgg cagcacttta tccagcaggg
    65821 cgcggatcgc ctcttccggt tgcggcccct ggaagccatc taccggttgc ccgttctgga
    65881 acagatacac ggtcggaatc gcgcgcagac caaactgggc ggcaatcatc tgctccgcgt
    65941 cgcagtccag cttcgccaga ataaattgcc cgttgtactg cgccgcgagg ctttccagga
    66001 tcggggttaa ctgcaaacag tgctggctac gttcagacca aaaatagaac agcaccggag
    66061 tggtcatcga ctgttcaaga acctgttgca ggttagattc gttaatgttg acaatatttt
    66121 ctacggacat ggagtcgctc tctgttgtcg atattttctt tgacatgggg gcttaagcgc
    66181 gcgcttcaac tcacccctgc aatattttgt ccatcacgcg ccccggcagc aggcgcttaa
    66241 gcaccattac tgcccaggtc accagcgtta ccggatagcg catcttcggc ttctcgctaa
    66301 taaaagcatg gcgtactttg tccaccaccg cttccggtcc caacgtaaag cgggcggcga
    66361 tgccgggatt ttcgactggt ttatcacttt gcgtctggtt gacgttgtcg gtgaagcgag
    66421 tacgaatggg accgggttcg atcaggctga ctttaattcc gctgtggcgt agctccatgc
    66481 gcagcgcgtc tgaccacgcc tccagcgcat atttactggc cgcgtaagcg ccacgacccg
    66541 gcgtggagat taatcccatc accgatgatg tcatcacaat acgcccttca ccgtgcggta
    66601 acatcgcggg taacaggcgc atggtgagct ggtgtgcgcc gaaaaagttg gcggaaaact
    66661 gctgttccat ctgcgcacgg ctgatggtgg aaagggggcc atacatgccg aatccggcat
    66721 tgttaaagat cccatacaga caattatcgg tcagggcgat cacttcgtcg gctgcgcgat
    66781 caacactttc tggcgaatcc agatcgatca acacgccggt aaatcccatg ctgttcatac
    66841 gctcaacatc atccggtttc cggcaacctg ccagcacatg aaaaccctgg cgttttaatt
    66901 cgagcgcgct ttccaggcca attccactgg aacatccggt aattaagacc gatttttgca
    66961 taactttacc tgtcaggatc tccgttgctt tatgagtcat gatttactaa aggctgcaac
    67021 tgcttcgcca tccagtcggc aataaacggc tgggcgtcgc ggttgggatg aataccgtca
    67081 tcctgcatcc attgtggctt gaggtagacc tcttccataa aaaagggcag cagcggaaca
    67141 tcaaactctt tggcgagttt ggggtaaatg gcgctaaagg cttcattata acggcgacca
    67201 tagtttgcag gcagacgtat ttgcattaac aatggttcag cgttggcggc tttgacatcc
    67261 tgcaaaatct ggcgcagcgt ttgctcggtt tgctgtggct gaaaaccacg caaaccgtca
    67321 ttgccgccca gttcaaccag cacccaacgc ggctgatgct gtttcagcag agccggaagg
    67381 cgcgccagtc cttgttgcga ggtgtcgccg ctgatgctgg cattaactac cgacgtttta
    67441 ctctgccact tatcattcaa caaggcaggc caggccgcgc tggcagacat tcgatacccg
    67501 gcgctcaggc tatcacccag aatcaataac gtgtccgctg cggcggcacg gaaggttaac
    67561 aggaccagga acaggaaggg caaatgccag cggaaaacat tgttgaagtt catcatctta
    67621 agaagtccgt cggtcagggg gagcatgaac tctccatcct caccggagtt gagctggttg
    67681 tcaaacgtgg cgagaccatc gcactggtgg gcgagtcggg atcgggtaag tcaaccttgc
    67741 tggcgatcct cgccgggctt gatgacggca gcagtggcga agtgagtctg gtgggacaac
    67801 cgctacataa tatggacgaa gaagcgcggg caaagttgcg cgcgaagcac gtcggctttg
    67861 tttttcagtc atttatgtta attcctaccc ttaacgcgct ggaaaacgtc gagcttccgg
    67921 ctctgctgcg cggtgagagt agcgcggaaa gtcgtaacgg ggcgaaagcg ttgctcgaac
    67981 agttagggct gggtaaacgt ctggatcatc ttccggcaca gctttccggc ggtgaacagc
    68041 aacgagtggc gctggcacga gcctttaatg gtcgacctga tgtgctgttt gccgacgaac
    68101 ccaccggcaa ccttgaccgc cagacgggcg ataaaattgc cgacctgctg ttttccctca
    68161 accgtgaaca tggcaccacg ttgattatgg tgacccacga cctgcaactg gcggcacgct
    68221 gcgaccgctg cttacggctg gtgaacgggc agttgcagga ggaagcatga ttgcacgttg
    68281 gttctggcgc gaatggcgtt cgccgtcgct attaattgtc tggctggcgc taagcctggc
    68341 ggtggcctgc gtgctggcgc tgggcaatat cagcgatcgc atggagaagg gcttaagcca
    68401 gcaaagccgt gagtttatgg cgggcgatcg ggcgttgcgc agttcacgcg aagtgccgca
    68461 agcgtggctg gaggaagcgc aaaagcgcgg cctgaaagtc ggcaagcagc tgactttcgc
    68521 cacaatgacc tttgcaggcg acacaccgca gctggcgaac gtcaaagcgg tggatgatat
    68581 ctacccgatg tatggcgatc tgcaaactaa tccccctggc ctgaaaccgc aggcgggcag
    68641 cgtattgctg gccccacgcc tgatggcact gcttaacctg aaaacgggcg acaccattga
    68701 cgtgggcgat gccaccttgc ggattgccgg agaagtgatt caggaaccgg attccggttt
    68761 taaccccttc cagatggctc cgcgtctgat gatgaatctg gcggatgtcg ataaaaccgg
    68821 agccgtgcag ccggggagtc gggtcacctg gcgttataaa ttcggcggca acgagaacca
    68881 gctcgacggc tatgagaaat ggttgttacc tcagcttaaa cccgaacaac gctggtacgg
    68941 tctggaacag gacgaaggcg cgctggggcg atcgatggaa cgctcgcaac agttcctgct
    69001 gctttcggcg cttctgacct tgctgctggc agtggcagcg gtggcggtag cgatgaatca
    69061 ttactgtcgc agtcgctacg atctggtggc gatcctcaaa acgctggggg cagggcgagc
    69121 gcaactgcgt aagctaatcg tcggtcagtg gttgatggtg ctgacgcttt cagccgttac
    69181 cggtggggcc ataggtctgt tgttcgaaaa cgtgttgatg gtgctgctca agcccgttct
    69241 gcctgctgca ctaccgccag ccagcctctg gccgtggctg tgggcgcttg gcaccatgac
    69301 ggtcatctcg ctgctggtgg ggctacgacc ataccgcttg ttgctggcaa cgcagccttt
    69361 acgcgtatta cgtaatgatg tggtagcgaa cgtctggccg ctgaagtttt atctgccgat
    69421 tgtcagtgtg gtggttgtgc tgctgctcgc cggattaatg ggtggcagca tgctgctttg
    69481 ggcggtgctg gcgggcgcgg tagtactggc tttgctgtgc ggtgtgctgg gctggatgct
    69541 gctgaatgta cttcgccgca tgacgctgaa atcgctgcct ctgcgcctgg cggttagccg
    69601 cctgttacgt cagccgtggt caacgttaag tcagctttcg gcattttcgc tctcctttat
    69661 gctgctggcg ctgttgctgg tattgcgtgg cgatctgctc gaccgctggc agcagcagct
    69721 accgccagaa agcccgaact actttttaat taacatcgcc acagaacagg tagcaccgct
    69781 aaaagcgttc ctcgcggaac atcatatagt cccggaatca ttttatccgg tagtgcgggc
    69841 gcggctgacg gcgattaacg ataagccgac agaaggtaat gaagatgagg cgcttaatcg
    69901 cgaactcaat cttacctggc aaaatacgcg gcccgatcat aatccgattg tcgccggtaa
    69961 ctggccgcca aaagccgatg aagtgtcgat ggaagagggg ctggcgaaac gcttaaacgt
    70021 tgccctcggt gataccgtga cttttatggg cgatacccag gagttccgcg ctaaagtgac
    70081 cagcctgcgc aaagtggact gggaaagtct gcggcctaat ttctatttta ttttcccgga
    70141 aggggcatta gacgggcaac cgcagagctg gcttaccagt ttccgctggg agaatggcaa
    70201 cggcatgttg acgcaactca accgccagtt cccgaccatt agcctgttag atattggcgc
    70261 gattttaaaa caggtcggtc aggtgctgga gcaggtaagt cgggcgctgg aggtgatggt
    70321 ggtgctggtc accgcctgcg gtatgttgct gttgctggca caggtgcagg tgggaatgcg
    70381 ccagcgtcat caggagctgg tggtatggcg cacactcggt gcggggaaaa aactgctgcg
    70441 cacgacattg tggtgtgagt tcgccatgct tgggtttgtt tccggcctgg tggccgcaat
    70501 tggtgcggaa acggcactgg cagtgttgca ggcgaaagtg tttgattttc cgtgggagcc
    70561 agactggcga ttgtggattg ttctgccgtg cagcggtgcg ctgctgctgt cgcttttcgg
    70621 cggctggctg ggtgcgcgac tggttaaggg taaggcgctg ttcaggcagt ttgcggggtg
    70681 atgacaggcg ttagattgcc tgatgcgcta cgcttatcag gcctacgaag agcgtgcaat
    70741 atattgattt ttcaagattg tgtaggccgg ataaggcgtt tacgccgcat ctggcatgaa
    70801 cagtgtgcat tttggcaata aatatgaaac caactgtcat gtcggttttt ttaaaggccg
    70861 aaggtggtaa accttcggcc ttagctacgt ttcctcagtt attcaataaa ctgacggatc
    70921 agttccagca gcgcgattag cgccttaaga acgatgagaa ctttatcgag aaaagttttc
    70981 atccgttgct cccttaaggg agaaagacaa tcacagctta cccttacgtt gcctgtaact
    71041 atgattttct tcatgtgcta gatgaggact gcctctctcc aaattagagc accagaatca
    71101 gcaccacctg tatcctgacc aggacttaaa agtacttttc cgcactgcgg taatcccttg
    71161 aggcagtatc actactagcg ttgggattgt atcgccaaca tagctgtttg aaatgtaggc
    71221 atcctgactt ataattcact tgctagatga ggactgtttc atttctgccc ggaaccacca
    71281 agtcttgggc ggaaaaaata aaaaaaccga ctgtcatgtc ggttttttta tatctgtatt
    71341 ctggtgcaac cattctgttc tcacgacaat cgccgtttca gaataccgcc catcactgcg
    71401 ccttaaccca ttccgccact tccgcccact caccgcgaaa gacaactttt tccgcttttt
    71461 tctcaagctg atagcgatac atcgggtcgt aatattcttc aagtaacggc accagccagg
    71521 ccagatgacc gtcggtgctg ccggtggtga gttgcgttgt cagtgctgca tccagccttg
    71581 cagccagttc gttatatcgc tgtagcccca gccgacgctt aatcgccgaa agtccgtgat
    71641 gcaggtattc gcaatattcc tgccagccct gttcgtcgcc ataagcgtgg gtaaaatcat
    71701 gatgcatacg caagaaatac tcttcgttca ggcgttcaag acggatctca aacggatctt
    71761 ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag acattccggc aggtgattcg
    71821 aaccgatcat ccggctttcg tcttccagca cccacaggcg caaattctga cgggcgtcgg
    71881 tttttagcat ttcggcagcc agcaggtttt caaaactcgc ctggctaagt tgtggttgta
    71941 aggtgcgacc aaacgccgaa ccgcgatgac gcgccaaccc ttccagatca acaccgttcg
    72001 gctgttgctg cactaacagc gttttgccgc agccggtaca accgccaatc agcactatcg
    72061 gtttttgtga cagttcaatg gtcgcctgaa tcgcagtctg gcgcagtacc ttataaccgc
    72121 cttccaccag cggataatta atccccactt catgcaacca gcgttgcaca atatgtgaac
    72181 gttgaccgcc acgggcgcag cagagaatac cttgcggatt ttgcaggcac gctgcccgcc
    72241 aggcgtccat gcgctgctga cgaatttcac ccgccaccag tttatgtccc agcgccagcg
    72301 ctgcgtctga gccttgctgt ttatagcagg tgccaacggc ggcgcgttca tcgttattca
    72361 ttaacggcag attgatagcg gcgggcattg cgccgtgctc aaactcgata ggggcgcgaa
    72421 catcaataat gggcgtatca gcaatcagca gggcacgata gtcctgttcc gtgtgtctct
    72481 cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc tattttacgc gccaacgcgc
    72541 aaggaaactt gatttttaac tgcgtgggtt gccgaaaatt tctaaaaatc cgctgatttc
    72601 cggcctgcgt tgggtaaaca aggtcacaat atcttctacc gctttgccgc tgccaaattt
    72661 gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt gggattaccc ggctgaccag
    72721 ttgttgatta tcgatcattg actggcaaag tgattttggc aaaaaaccaa tgccaacgcc
    72781 cgccagatgg gcggcgattt tcgtttccat atcaggaaca ataatctctt tttgccctgg
    72841 caatcgccag gcgacgcgtt tggttaaggt gcgggcgctg tcttcaatat tgaccgccgg
    72901 aaagcgccgc aactgcgctt ctgttagcgg ctcttcaacg ttcgccagcg gatgatccgc
    72961 cgccatgaca aagcgccatt gcaccgatcc taagggatca agactaaagg tatttgccag
    73021 cgcctcggtt cccgtgacgc cgatagccag tgaaaaacct tcgtacaata gcgagtccca
    73081 gacgcccata tagatttgtc gggagatgtg aaactgggta aaggggtaac gttcattcag
    73141 ccacgccagc aactgggcga cggcctgggg gttgtagagc aggttgttga tgacaatatt
    73201 cacctggcgt tccacgccat cattcacctg ttgcagctcg cttggcatac tttccagcca
    73261 gctcagccag tctctggcct gggaaagtag atgctcgcca gccgctgtca acgtcacgct
    73321 gcgagtcgtg cggaaaaaca gcgctactcc ggtattctct tccagaagtt taatgcgata
    73381 actgatcgtc gctgtggttt tacataatcg ttctgccgct tttgaaaaac ttcctgtttc
    73441 agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac atcttcaggt atcccctttt
    73501 aaatccgcaa gttgcgtgat tttcttatcc tctgatttat cagtattttt acatgataac
    73561 cctgttcaat ttgtggacta aatctagttt tggaaaaata ttccaacttt tgtattgaag
    73621 ttgttctctt aaggttttag attgcctgtt attgaaacca agctgaccgg tcggcggtgg
    73681 ttgaacggaa ttatgttaca aggacaaaaa gatgaaactt caggtattac cgttaagtca
    73741 ggaagccttt agtgcttatg gcgacgtaat cgaaacgcag caacgggatt ttttccatat
    73801 taacaatggc ctggtggagc gttaccacga tttggcgctg gttgagattc ttgagcaaga
    73861 ccgtacgctt atcagcatta accgcgcgca accggcgaat ctaccgctga ccattcacga
    73921 actcgaacgt catccgctgg gtactcaggc ctttatcccg atgaaaggtg aagtgtttgt
    73981 ggtggtcgtg gcgttaggtg acgacaaacc agacctgtca accctgcggg cgtttatcac
    74041 caacggtgaa cagggggtga attaccatcg taacgtctgg catcacccac ttttcgcctg
    74101 gctgcgcgtc accgattttc tgaccatcga tcgcggcggc agtgacaact gtgatgttga
    74161 aagtattcct gaacaggaac tctgttttgc gtgacgcctg caaccgactt gcataagata
    74221 aattaattgt tcattgttta tgctcacttg taggtcggag ttaacgtagg tatgacggaa
    74281 gttagacggc gcggcaggcc aggacaggcg gagcctgtgg cacagaaggg cgcacaggcg
    74341 ttagagcggg gaattgcgat tctgcaatat ctggaaaaaa gtgggggaag ttcgtcggtt
    74401 agcgatattt ctctcaatct ggatttgccg ctctccacga cctttcgctt gctgaaggtt
    74461 ttacaggcag cggattttgt ctatcaggac agtcagttag gctggtggca tataggatta
    74521 ggtgtcttta acgtcggtgc ggcgtacatc cataaccgcg atgtcctctc cgtcgccggg
    74581 ccgtttatgc gccgcctgat gttactttcc ggcgaaacgg tcaatgtcgc gatccgtaac
    74641 ggcaatgaag cagtattaat tggtcagcta gagtgtaaat cgatggtcag gatgtgtgcg
    74701 ccactgggca gtcgtctgcc actgcatgct tccggtgcgg gcaaagcgct gctttatccg
    74761 ctggcggaag aggagttgat gagcatcatt ctgcaaaccg gtttgcagca gtttacgcca
    74821 actacgcttg tggatatgcc caccttactg aaggacctgg agcaagcgcg tgaactgggc
    74881 tataccgtag ataaagaaga gcatgttgta ggtctgaatt gcatagcttc agcaatttac
    74941 gatgatgtag gtagtgttgt tgccgctatc tccatctccg ggccttcatc aagactgaca
    75001 gaagatcgtt ttgtcagtca gggtgagctg gtcagagaca ccgcccgcga tatcagcacg
    75061 gcgttgggac tgaaagcaca tccatagtgt ctgtcgcatc ccgctctgcg gagcgggttt
    75121 ttttgacaaa atttgaaagt tggaaaattt ttccaattaa tagaggtagg aataaaatgg
    75181 caaaaatgag agccgttgac gcggcaatgt atgtgctgga gaaagaaggt atcaccaccg
    75241 ccttcggcgt tccgggagct gcaatcaatc cgttctactc agcgatgcgt aagcacggcg
    75301 gtattcgtca cattctggcg cgtcatgtgg aaggtgcttc gcacatggcg gaaggttata
    75361 cccgcgcaac ggcagggaat atcggcgtat gtctggggac ttccggtcct gcgggcacgg
    75421 acatgatcac cgcgctctat tccgcttctg ctgattccat tcctattctg tgcattaccg
    75481 gccaggcacc gcgcgcccgt ctgcataaag aagattttca ggccgtagat attgaagcaa
    75541 ttgctaaacc ggtcagcaaa atggcggtta cagttcgtga agcggcgctg gtgcctcgcg
    75601 tgctgcaaca ggcatttcac ctgatgcgtt ctggtcgtcc gggtccggta ctggtggatt
    75661 taccgttcga cgttcaggtt gcggaaatcg agtttgatcc tgacatgtac gaaccgctgc
    75721 cggtctacaa acctgctgcc agccgtatgc agatcgaaaa agctgtagaa atgttaatcc
    75781 aggccgaacg tccggtgatt gttgccgggg gcggggtaat taatgctgac gcagctgcac
    75841 tgttacaaca gtttgctgaa ctgaccagcg ttccggtgat cccaacgcta atgggctggg
    75901 gctgtatccc ggacgatcat gaactgatgg ccgggatggt gggtctgcaa accgcgcatc
    75961 gttacggtaa cgcaacgctg ctggcgtctg acatggtgtt tggtatcggt aaccgttttg
    76021 ctaaccgtca taccggctcg gtagagaaat acaccgaagg gcgcaaaatc gttcatattg
    76081 atattgagcc gacgcaaatt ggtcgcgtgc tgtgtccgga tctcggtatt gtctctgatg
    76141 ctaaagcggc gctgacactg ctggttgaag tggcgcagga gatgcaaaaa gcgggtcgtc
    76201 tgccgtgtcg taaagaatgg gtcgccgact gccagcagcg taaacgcact ttgctgcgca
    76261 aaacccactt cgacaacgtg ccggtgaaac cgcagcgcgt gtatgaagag atgaacaaag
    76321 cctttggtcg cgatgtttgt tatgtcacca ccattggtct gtcacaaatc gctgcggcac
    76381 aaatgctgca tgtctttaaa gaccgccact ggatcaactg tggtcaggct ggtccgttag
    76441 gctggacgat tccggctgcg ctaggggttt gtgccgctga tccgaaacgc aatgtggtgg
    76501 cgatttctgg cgactttgac ttccagttcc tgattgaaga gttagctgtt ggcgcgcagt
    76561 tcaacattcc gtacatccat gtgctggtca acaacgctta tctggggctg attcgccagt
    76621 cgcagcgcgc gtttgatatg gactactgcg tgcaactcgc tttcgagaat atcaactcca
    76681 gcgaagtgaa cggttacggc gtcgaccacg taaaagtagc ggaaggttta ggttgtaaag
    76741 cgattcgcgt cttcaaaccg gaagatattg cgccagcctt tgaacaggcg aaagccttaa
    76801 tggcgcaata tcgggtaccg gtagtcgtgg aagttattct cgagcgtgtg accaatattt
    76861 cgatgggcag cgaactggat aacgtcatgg aatttgaaga tatcgccgat aacgcagcgg
    76921 acgcaccgac tgaaacctgc ttcatgcact atgaataagg gagataaata atgttacgtt
    76981 tctctgctaa tttatcgatg ttatttggag aatatgattt tctcgcccgt tttgagaaag
    77041 ctgcgcagtg tggttttcgc ggcgttgaat ttatgtttcc ttatgactac gacattgaag
    77101 aattaaaaca ggtgctggcg agtaataaac tcgaacatac gctgcacaat ctaccggcgg
    77161 gtgactgggc ggcgggggag cgcggtattg cctgtattcc tggccgtgaa gaagagtttc
    77221 gggatggcgt agcagcagcg attcgttatg cccgtgcgct ggggaataaa aaaattaact
    77281 gtctggtcgg taaaacgccg gctggtttca gcagtgaaca gattcacgca acgcttgtag
    77341 aaaacctgcg ttatgccgcg aatatgctga tgaaagaaga tattttatta ctgattgaac
    77401 ctattaacca ttttgatatt cctggtttcc atctcaccgg aactcggcag gcgctgaaat
    77461 tgattgatga tgttggttgc tgcaatttga aaattcagta tgacatttat cacatgcagc
    77521 ggatggaagg tgaattaacc aacaccatga ctcagtgggc cgataaaatt ggccacctgc
    77581 aaattgctga taatccgcat cgcggcgaac cggggaccgg agaaattaat tatgattatc
    77641 tctttaaggt aatcgaaaat tctgactaca acggttgggt tgggtgtgaa tataaacccc
    77701 aaaccaccac ggaagccggt ttacgctgga tggatccgta ccgttaaaac gtaacgctgt
    77761 tcaggcaatg cttttttagg ccgctaagtt ggcaggggat cgtgttgtct gaattcagga
    77821 aaagcgaaat ttaaaagagg ttaattatga aactgggatt tattggctta ggcattatgg
    77881 gtacaccgat ggccattaat ctggcgcgtg ccggtcatca actgcatgtc acaaccattg
    77941 gtccggttgc tgatgaatta ctgtcactgg gtgccgtcag tgttgaaact gcgcgccagg
    78001 taacggaagc atcggacatc atttttatta tggtgccaga tacaccacag gttgaagaag
    78061 ttctgttcgg tgaaaatggt tgtaccaaag cctcgctgaa gggcaaaacc attgttgata
    78121 tgagctccat ttccccgatt gaaactaagc gtttcgctcg tcaggtgaat gaactgggcg
    78181 gcgattatct cgatgcgcca gtctccggcg gtgaaatcgg tgcgcgtgaa gggacgttgt
    78241 cgattatggt tggcggtgat gaagcggtat ttgaacgtgt taaaccgctt tttgaactgc
    78301 tcggtaaaaa tatcaccctc gtgggcggta acggcgacgg tcaaacctgc aaagtggcaa
    78361 atcagattat cgtggcgctc aatattgaag cggtttctga agccctgcta tttgcttcaa
    78421 aagccggtgc ggacccggta cgtgtgcgcc aggcgctgat gggcggcttt gcttcctcac
    78481 gtattctgga agttcacggc gagcgtatga ttaaacgcac ctttaatccg ggcttcaaaa
    78541 tcgctctgca ccagaaagat ctcaacctgg cactgcaaag tgcgaaagca cttgcgctga
    78601 acctgccaaa cactgcgacc tgccaggagt tatttaatac ctgtgcggca aacggtggca
    78661 gccagttgga tcactctgcg ttagtgcagg cgctggaatt aatggctaac cataaactgg
    78721 cctgataccc gcaataaaaa tggccgatat cagaaaatga atcggccagc aatattaaaa
    78781 aagaaagcag ccaaagatgt tgcttcagta ttaaaaataa tatttttatt ttatttgttc
    78841 ctcatagcta gattaaaaca acgttattcg atacgtgaaa ttaagaggga tttatggaac
    78901 atcagagaaa actattccag caacgcggct atagcgaaga tctattgccg aaaacgcaaa
    78961 gccagcggac ctggaaaaca tttaactatt ttaccttatg gatgggttcg gttcataacg
    79021 ttcccaatta tgtgatggtc ggcggctttt ttattctcgg cttgtctacc tttagtatta
    79081 tgctggcaat tatcctcagc gcctttttca ttgccgcggt aatggtatta aacggcgctg
    79141 cgggcagtaa atacggcgta ccgtttgcca tgatcctgcg tgcttcttac ggcgtacgtg
    79201 gcgcactgtt tcccggatta ttaagaggcg gtattgctgc cattatgtgg tttggcctgc
    79261 aatgttacgc ggggtcactg gcctgcttga ttctgattgg caaaatctgg ccgggatttt
    79321 taactctcgg tggtgatttc acgctgttag ggctttctct accgggctta attactttct
    79381 tactcttctg gctggtcaac gttggtatag gttttggcgg tggcaaagtt ttaaataaat
    79441 tcactgccat tcttaacccg tgcatctata tcgttttcgg cggtatggcg atttgggcga
    79501 tttcgctggt cgggctcggt ccaatctttg actacattcc gagcggtatt cagaaagcag
    79561 aaaacagtgg cttcctgttc ctggtggtga taaacgcggt agttgcggtc tgggcggcac
    79621 cggcggtgag cgcatccgac tttacgcaaa acgcccactc gtttcgtgag caagcgctgg
    79681 ggcaaacgct gggtttagtt gtggcctata ttctgtttgc ggtcgcgggg gtatgtatta
    79741 ttgccggagc cagtattcat tacggcgctg atacctggaa cgtgctggat attgttcagc
    79801 gttgggacag cttgttcgcc tcgttctttg cggtactggt tattctgatg acgactatct
    79861 ccactaacgc caccggtaat attattcctg ccggttatca gattgccgct attgcaccga
    79921 cgaaactgac ttataaaaac ggcgtactga ttgccagtat tatcagcctg ctgatctgcc
    79981 cgtggaaatt aatggaaaat caggacagta tttatctgtt cctcgatatt atcggcggaa
    80041 tgcttggtcc ggtaattggt gtcatgatgg cgcattattt tgtggtgatg cgtgggaaaa
    80101 ttaatcttga tgaactgtat accgcacctg gcgattataa atattacgat aacggtttta
    80161 acctcactgc gttttcagta actctggtgg ccgttatttt atctcttggc ggtaagttta
    80221 ttccctttat ggaaccgtta tcgcgtgttt catggtttgt cggcgtcatc gtcgcctttg
    80281 cggcctacgc cttattaaag aaacgtacaa cagcagaaaa aacaggagag caaaaaacca
    80341 taggttaatt aatcccgata ttgaacattg agttaaaaac caatctgtat tttacaagga
    80401 gtttgttatg tcttttgatt taatcattaa aaacggcacc gttattttag aaaacgaagc
    80461 tcgcgttgta gatatcgccg ttaaaggcgg aaaaattgct gctatcggtc aggatctggg
    80521 cgatgcaaaa gaagttatgg atgcgtctgg tctggtggtt tcgccgggca tggttgatgc
    80581 gcacacccat atttctgaac cgggtcgtag ccactgggaa ggttatgaaa ccggtactcg
    80641 cgcagcggca aaaggtggta tcaccaccat gatcgaaatg ccgctcaacc agctgcctgc
    80701 aacggttgac cgtgcttcaa ttgaactgaa gttcgatgcc gctaaaggca agctgactat
    80761 cgatgcggca caactcggtg gcctggtgtc ttacaacatt gatcgtctgc atgagttgga
    80821 tgaagtgggc gttgtcggct tcaaatgctt cgttgcgacc tgtggcgatc gcggtatcga
    80881 caacgacttc cgtgatgtaa acgactggca gttcttcaaa ggtgcgcaga agctgggcga
    80941 actggggcag ccggtgttgg tgcactgcga aaacgcgctg atctgtgacg cactgggcga
    81001 agaagcgaaa cgtgaaggtc gcgtaactgc ccatgactat gtggcttcgc gtccggtatt
    81061 taccgaagtg gaagcgattc gccgcgtgct gtatctggcg aaagttgccg gttgccgtct
    81121 gcacgtttgc catatcagca gcccggaagg tgttgaagaa gtgactcgtg cacgtcagga
    81181 aggtcaggat gttacctgtg aatcctgccc gcattacttt gtactggata ccgatcagtt
    81241 cgaagaaatc ggcactctgg cgaagtgttc accgccgatc cgcgatctgg aaaaccagaa
    81301 aggcatgtgg gaaaaactgt ttaacggtga aatcgactgc ctggtttccg accactcacc
    81361 atgccctccg gaaatgaaag ccggcaacat catggaagca tggggcggta ttgccggtct
    81421 gcaaaactgt atggacgtga tgttcgatga agcggtacag aaacgcggaa tgtctctgcc
    81481 aatgttcggc aaattaatgg cgactaacgc agcagatatt ttcggtctgc agcaaaaagg
    81541 ccgtatcgcc ccaggaaaag atgccgactt cgtcttcatt cagccgaata gcagctatgt
    81601 tcttaccaat gacgatctgg aatatcgcca caaagtcagc ccgtatgttg gccgtaccat
    81661 tggcgcgcgt atcacgaaaa ccatcttacg tggtgatgtg atttacgaca ttgaacaggg
    81721 cttccctgtt gcgccgaaag gtcaatttat ccttaaacat cagcagtaat ctggcccctg
    81781 caatgcccgt ccttgcggcg ggcattctcc ggttaaggtg tgtttatgtt caattttgca
    81841 gtcagccgcg aaagcctgtt atcaggattt cagtggtttt tctttatttt ttgcaacacg
    81901 gttgtggttc ctcctacgct actttctgct tttcagttgc cgcaaagtag cctgcttacg
    81961 ctcacgcaat atgcttttct tgctaccgca ctggcctgct tcgctcaggc gttctgcggt
    82021 catcgtcgcg ctattatgga agggccgggt ggcctgtggt ggggaaccat ccttactatc
    82081 acccttggcg aagcatcgcg cgggacaccg atcaacgata tcgctaccag cctggcggtg
    82141 gggattgcgc tctccggcgt gctgacgatg ttgattggtt ttagcggatt aggccatcgc
    82201 ctggcacggt tatttacgcc gtcggtgatg gtcttgttta tgttgatgct gggcgcgcag
    82261 ctgaccacta tctttttcaa aggtatgctc gggctgccgt ttggcatagc cgacccaaat
    82321 tttaaaattc agttaccgcc gttcgcgctc tcggtggcgg tgatgtgcct ggtactgggg
    82381 atgattatct tcctgccgca acgttttgcc cgttatggcc tgctggtcgg caccataacc
    82441 ggctggttgt tgtggtactt ttgctttcct tcttcgcact cgctctccgg tgagttgcac
    82501 tggcagtggt tcccgctcgg cagtggaggt gctttgtctc cgggaattat tctgacggcg
    82561 gtgattacag gtctggtgaa tatcagcaat acctacggtg cgattcgggg cacggatgtt
    82621 ttttatccgc agcagggcgc gggaaatacg cgttatcgtc gcagctttgt ggcgaccgga
    82681 tttatgacgc tgataaccgt accgctggcg gtaattccat tttcaccgtt tgtttcatcc
    82741 attggtttat taacccagac aggcgattac acgcggcgtt cgtttattta tggcagcgtt
    82801 atttgcctgc tggtggcgct ggttccggca ctcacacgac tgttttgcag tatcccttta
    82861 cccgtgagta gcgcggtcat gctggtttct tatctgcctt tactcttttc cgcgctggtg
    82921 tttagccagc aaataacctt taccgctcgc aatatttatc gactcgcatt gccgttattt
    82981 gtcggcatat ttttaatggc attaccgcct gtgtatctgc aagaccttcc attaacgctt
    83041 cgtcctctgc tcagtaacgg cttattggtc gggattttac tggctgttct tatggataac
    83101 cttattccgt gggaacgtat cgaataattt gttgaaaaag gattgataat gaagattgtc
    83161 attgcgccag actcttttaa agagagctta agtgcagaaa aatgttgtca ggcaattaaa
    83221 gccgggtttt cgaccctctt tcccgatgcg aactatatct gtttgccgat agcggatggc
    83281 ggcgaaggaa cggtggatgc gatggtcgcc gcgacgggcg gcaacatcgt gacgcttgaa
    83341 gtctgcgggc cgatgggcga aaaagtgaat gctttttatg gccttaccgg cgacgggaaa
    83401 acggcggtga ttgagatggc ggcagcaagt ggcctgatgc tggtcgcgcc tgaaaagcgt
    83461 aatccgttgc tggcctccag ttttggtacg ggggagttaa ttcgtcatgc gctggataac
    83521 ggcattcgcc atattattct cggcattggc ggcagtgcga cggtcgacgg cggtatgggc
    83581 atggcgcagg cgcttggtgt gcgtttcctt gatgccgacg gccaggtgct ggcggctaac
    83641 ggcggcaatt tagcgcgcgt ggcaagcatt gagatggagg aatgcgatcc gcgtctggcg
    83701 aacagccata ttgaagtggc atgtgacgtt gataacccgc tggtaggggc acgcggcgcg
    83761 gcggcggtat ttggcccgca aaaaggggca acgccggaga tggtcgaaga gctggaacag
    83821 gggctgcaaa attacgcccg tgttttacaa cagcaaactg aaattaatgt ctgccagatg
    83881 gcgggcggcg gcgctgcggg cggtatgggt attgcggcgg cggtatttct caatgcggat
    83941 attaaaccgg gcattgaaat tgtgttgaat gcagtcaatc ttgcgcaggc agtgcagggc
    84001 gcagcactgg tgatcaccgg ggaagggcgc atcgactcgc aaacggcagg cggtaaagcg
    84061 ccgctgggtg tggcgtcggt ggcgaaacag tttaatgtac cggtgattgg aattgctggc
    84121 gtattgggtg atggcgtgga agtggtacac cagtacggca ttgacgcggt attcagcatt
    84181 ttgccacgtc tggcaccttt agccgaagtg ctcgccagcg gtgaaaccaa tctcttcaac
    84241 agcgcgcgaa atattgcctg cgccattaaa ataggtcagg gaattaaaaa ttgactcttg
    84301 cctttaaagc ggatgctatt tatttcgtat aagagcgcag tgctcatgcc ggatgcggca
    84361 tgagtactat atccttcctg aaaatcgcgc aaattctata tattgcagag atcatgtagg
    84421 cctgataagc gaagcgcatc aggcaatgtt acaaaaaaag ccacggtata aaccgtggca
    84481 aaatccaaca tagctaaaaa taatcaggcg agtggtatga cttaaatctc tacgtcgcgg
    84541 ttgcaatctt tcgagtaaat atagctgaac gcttcaccac gccctacacc ataaccagcc
    84601 tgtaaagaat aagcgcccat aaagatgtaa tcgccttttt tcaccgggat ccagttattg
    84661 tcgaggttat aaaccccctg accggaaaga atataggcac cgtgttcctg aacgtgtgtt
    84721 tcgatataac cgtggctggc acctggtgca aaagagagga tatgcatgtt catatcaaaa
    84781 cctaactctt tgggcagaaa atccagcaga ataacatcgt ccataccttc atagtgaatg
    84841 cgttccagtt cgctggcatt gccagaaacc agccacggtg catagccttc taccggaaca
    84901 tagcggcgct tatataaaaa gatttggctg tcttcggcct gggcgttaac aaacgtcatt
    84961 aaggagcctg gcgggcaata aagatagcca ccttcgctta aggcaaatgt tttgccttcg
    85021 gctttggcag tgatatttcc agagatcaca tacaggaacg tttcaatgcc ttcgccaccg
    85081 aagccctgtt ggttgccacc gttttgatgc agtgtgacca gataatcaac aaaagaggca
    85141 cccagctttg gcgtggagag gattgtcgcg tcacaatttt caaagcccgg aataatattt
    85201 tttaccagac cgtctggtgt taacagtgcg aaattaccgt gtttcacaat cgcacggtta
    85261 gccagtaaat catcgcggta accggtgacg ttatttaaat atcccattta taactcctta
    85321 tttctgccag gcaagttgat aaagcatgag tgccaacgtt ttgacccctt cggcaaggtc
    85381 ggtaatattg gtgcgttccg ccgggttatg gctgatcccg ttgatgctgg ggataaaaat
    85441 catgcaggtt ggtacgcgag gcgcgaaaat ttgcgcgtcg tgcccggcac cactgtgcat
    85501 cacccggtaa ttcagttttt ctctttcaca caattctgtc agggtggcga ccagctcctt
    85561 attcatcggc acgggttctt cgtccatcca taaatcgata tcaataccaa tgtccatttc
    85621 atcgcaaatc gcccgcatgt cgttttctaa ctgttgggtg aaatcacgca ggacggcggc
    85681 gtcggtatga cgacaatcaa tggtgaacgt ggttttaccc ggcaccacat tcaccgtatt
    85741 cgggcgcggc tccaccttac caaaggtcag aaccagcgga tcgcccatct ttttcgcttt
    85801 ttcgatcgac tgatggcaaa tgcgactgaa agcgtaaacc gtatcacgac gataacccat
    85861 cggcgtggtg cctgcatggt ttgattcgcc gttcagcgtc accgtatagc gacgctgccc
    85921 gacaattgca ttcaccacgc caattgattg accattactt tccagcacac agccctgttc
    85981 aatatgcagt tcaacaacag ctttaatatc ctgacgcgga gttagtgggg cgttcggcag
    86041 agtaaatccg caagccttca tcgcatcgac aaaactattt cctttggcat cacagatatt
    86101 ccgcacgtcg tcaggattcg ccagcccaaa gatattttta ctgccccaga agacatacgg
    86161 gaagcggctg ccttcttctt ctgccatcgc caccacttcg accgtacgta gcggcgcgcc
    86221 gtattgcgtt ttcagccagt caattgccag ccacgccgcc agcgcgccga attgcccgtc
    86281 gaggttaccg ccgttaacca cggtatcgat atgcgaaccg ctcagaatca cttcctgtgg
    86341 atattcggta ccattcaggc gaccgtataa attccctact tcatcgaaac gtgtttccag
    86401 cccacttgct gccattcttt ttttaaattg ctgctgggtt tccagccatt ccggcgaata
    86461 aagtaaacgg atcatcccac ccgttgggtc agcgccaaaa gaggaaagcc agggcagcgt
    86521 ttcttctatg gcttgacgaa aatgtgtaat cataagaaag tcctgtctca ataattattg
    86581 cgcaaaggga tttttcgttt cgtatgacgt gttataaagc gcgtcggaaa ttaaatactg
    86641 gtaaatatca tcagcaattt cgatgccttc gacggcggct ttgcgttgtt taatatcctg
    86701 atcctgtccg ggataataaa cttggttaaa accgggcgcg ggggtaatgg catttaattc
    86761 gcgcatggtc tggctaagat gttgacggaa taattcgctg gaggagaaaa agttcggatt
    86821 aataactata tgtaattgcc ccaaattacg ccctgcgtgt aaatcgtcat acatcgaact
    86881 aacctgtcgc ccgaacggta agccgagtaa gacgcctgag aggacgtcaa tcatcatcat
    86941 caggccatac ccttttggcc cggcggcggg gagcagagca tgtaccgcga acggatcggt
    87001 tgttggtgca ccgtttttat cgaccgccca ggtatccggg atagacatat tacgcgagcg
    87061 ggcgtcgagc acttttcccc atgcctgtac ggtagtcgcc atatcaaagg taaggatctc
    87121 gtcgccttct cccggcgcgg caaaggccag ggggttagta ccgtagtaaa tttccgcgcc
    87181 gccaaacggc accaccattg gatcggactg gcacatcgaa atgccaatga atccggcgcg
    87241 ggctgcctgc tgcacaaaat aagagattgc gccgctgtga cccatccggc tgataccgac
    87301 caccgcaacg ccattttgct gggcggtttt gatggcatgt tccataccca ttttcgccgc
    87361 gacctgtccg gcggcattgt cggcatgtaa aattgccgag cacggcccgg tttcctcaag
    87421 acgaaactcc ggttcgcggt tggtgccgcc ttttgaaatg cgttccgcgt agtattccac
    87481 gcgcaccgcg ccatgagagt ggatccctct ggcatcggcg taaaccaata cttcagccac
    87541 ggttgcagcg tgctcacgtt ttaacccagc ctggcagagt ttattctcaa ttagctggtg
    87601 gagtgtttcc cgattgattt tcatctgtct tcctttttaa cgacggtgtg aagcatgact
    87661 gcaattaaca tacagggaaa atatctggat tatgtgatcc agacaggca
//
LOCUS       DAFNBJ010000003        72300 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000003 DAFNBJ010000000
VERSION     DAFNBJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.003"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(131..706)
                     /gene="gmhB"
                     /locus_tag="L2X49_000197"
     CDS             complement(131..706)
                     /gene="gmhB"
                     /locus_tag="L2X49_000197"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBN4795350.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            894..1925
                     /gene="metN"
                     /locus_tag="L2X49_000198"
     CDS             894..1925
                     /gene="metN"
                     /locus_tag="L2X49_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBN4795351.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            1918..2571
                     /gene="metI"
                     /locus_tag="L2X49_000199"
     CDS             1918..2571
                     /gene="metI"
                     /locus_tag="L2X49_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBN4795352.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            2611..3426
                     /gene="metQ"
                     /locus_tag="L2X49_000200"
     CDS             2611..3426
                     /gene="metQ"
                     /locus_tag="L2X49_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBN4795353.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            3544..3948
                     /gene="rcsF"
                     /locus_tag="L2X49_000201"
     CDS             3544..3948
                     /gene="rcsF"
                     /locus_tag="L2X49_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBN4795354.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            3945..4652
                     /gene="tsaA"
                     /locus_tag="L2X49_000202"
     CDS             3945..4652
                     /gene="tsaA"
                     /locus_tag="L2X49_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBN4795355.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            4764..6482
                     /gene="proS"
                     /locus_tag="L2X49_000203"
     CDS             4764..6482
                     /gene="proS"
                     /locus_tag="L2X49_000203"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBN4795356.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVNAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            6535..7358
                     /locus_tag="L2X49_000204"
                     /pseudo
     CDS             6535..7358
                     /locus_tag="L2X49_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(7513..8223)
                     /gene="nlpE"
                     /locus_tag="L2X49_000205"
                     /gene_synonym="cutF"
     CDS             complement(7513..8223)
                     /gene="nlpE"
                     /locus_tag="L2X49_000205"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBN4795357.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(8237..8659)
                     /gene="arfB"
                     /locus_tag="L2X49_000206"
     CDS             complement(8237..8659)
                     /gene="arfB"
                     /locus_tag="L2X49_000206"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBN4795358.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            complement(8656..9201)
                     /locus_tag="L2X49_000207"
     CDS             complement(8656..9201)
                     /locus_tag="L2X49_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBN4795359.1"
                     /translation="MVLKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     VLFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            9367..9567
                     /locus_tag="L2X49_000208"
     CDS             9367..9567
                     /locus_tag="L2X49_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBN4795360.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            9554..9814
                     /gene="rof"
                     /locus_tag="L2X49_000209"
     CDS             9554..9814
                     /gene="rof"
                     /locus_tag="L2X49_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBN4795361.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(9863..11161)
                     /gene="tilS"
                     /locus_tag="L2X49_000210"
     CDS             complement(9863..11161)
                     /gene="tilS"
                     /locus_tag="L2X49_000210"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBN4795362.1"
                     /translation="MTLTLNRQLLTLRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWINSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(11226..11615)
                     /locus_tag="L2X49_000211"
     CDS             complement(11226..11615)
                     /locus_tag="L2X49_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBN4795363.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(11672..13813)
                     /gene="ldcC"
                     /locus_tag="L2X49_000212"
     CDS             complement(11672..13813)
                     /gene="ldcC"
                     /locus_tag="L2X49_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBN4795364.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            complement(13912..14871)
                     /gene="accA"
                     /locus_tag="L2X49_000213"
     CDS             complement(13912..14871)
                     /gene="accA"
                     /locus_tag="L2X49_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBN4795365.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(14884..18366)
                     /gene="dnaE"
                     /locus_tag="L2X49_000214"
     CDS             complement(14884..18366)
                     /gene="dnaE"
                     /locus_tag="L2X49_000214"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBN4795366.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(18401..18997)
                     /gene="rnhB"
                     /locus_tag="L2X49_000215"
     CDS             complement(18401..18997)
                     /gene="rnhB"
                     /locus_tag="L2X49_000215"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBN4795367.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            complement(18994..20142)
                     /gene="lpxB"
                     /locus_tag="L2X49_000216"
     CDS             complement(18994..20142)
                     /gene="lpxB"
                     /locus_tag="L2X49_000216"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBN4795368.1"
                     /translation="MTKQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(20142..20930)
                     /gene="lpxA"
                     /locus_tag="L2X49_000217"
     CDS             complement(20142..20930)
                     /gene="lpxA"
                     /locus_tag="L2X49_000217"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBN4795369.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(20934..21389)
                     /gene="fabZ"
                     /locus_tag="L2X49_000218"
     CDS             complement(20934..21389)
                     /gene="fabZ"
                     /locus_tag="L2X49_000218"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBN4795370.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(21494..22519)
                     /gene="lpxD"
                     /locus_tag="L2X49_000219"
     CDS             complement(21494..22519)
                     /gene="lpxD"
                     /locus_tag="L2X49_000219"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBN4795371.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(22523..23008)
                     /gene="skp"
                     /locus_tag="L2X49_000220"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(22523..23008)
                     /gene="skp"
                     /locus_tag="L2X49_000220"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBN4795372.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(23130..25562)
                     /gene="bamA"
                     /locus_tag="L2X49_000221"
     CDS             complement(23130..25562)
                     /gene="bamA"
                     /locus_tag="L2X49_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBN4795373.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVINPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(25592..26944)
                     /gene="rseP"
                     /locus_tag="L2X49_000222"
     CDS             complement(25592..26944)
                     /gene="rseP"
                     /locus_tag="L2X49_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBN4795374.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(26956..27813)
                     /gene="cdsA"
                     /locus_tag="L2X49_000223"
     CDS             complement(26956..27813)
                     /gene="cdsA"
                     /locus_tag="L2X49_000223"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBN4795375.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(27826..28584)
                     /gene="ispU"
                     /locus_tag="L2X49_000224"
                     /gene_synonym="uppS"
     CDS             complement(27826..28584)
                     /gene="ispU"
                     /locus_tag="L2X49_000224"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBN4795376.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(28773..29969)
                     /gene="ispC"
                     /locus_tag="L2X49_000225"
     CDS             complement(28773..29969)
                     /gene="ispC"
                     /locus_tag="L2X49_000225"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBN4795377.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSCTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            complement(30061..30618)
                     /gene="frr"
                     /locus_tag="L2X49_000226"
     CDS             complement(30061..30618)
                     /gene="frr"
                     /locus_tag="L2X49_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBN4795378.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(30768..31493)
                     /gene="pyrH"
                     /locus_tag="L2X49_000227"
     CDS             complement(30768..31493)
                     /gene="pyrH"
                     /locus_tag="L2X49_000227"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBN4795379.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(31640..32491)
                     /gene="tsf"
                     /locus_tag="L2X49_000228"
     CDS             complement(31640..32491)
                     /gene="tsf"
                     /locus_tag="L2X49_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBN4795380.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(32749..33474)
                     /gene="rpsB"
                     /locus_tag="L2X49_000229"
     CDS             complement(32749..33474)
                     /gene="rpsB"
                     /locus_tag="L2X49_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBN4795381.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33842..34636
                     /gene="map"
                     /locus_tag="L2X49_000230"
     CDS             33842..34636
                     /gene="map"
                     /locus_tag="L2X49_000230"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBN4795382.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            34698..37370
                     /gene="glnD"
                     /locus_tag="L2X49_000231"
     CDS             34698..37370
                     /gene="glnD"
                     /locus_tag="L2X49_000231"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBN4795383.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            37401..38225
                     /gene="dapD"
                     /locus_tag="L2X49_000232"
     CDS             37401..38225
                     /gene="dapD"
                     /locus_tag="L2X49_000232"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBN4795384.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            38540..38926
                     /locus_tag="L2X49_000233"
     CDS             38540..38926
                     /locus_tag="L2X49_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBN4795385.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(39015..40172)
                     /gene="cdaR"
                     /locus_tag="L2X49_000234"
     CDS             complement(39015..40172)
                     /gene="cdaR"
                     /locus_tag="L2X49_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HBN4795386.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(40327..41751)
                     /gene="degP"
                     /locus_tag="L2X49_000235"
     CDS             complement(40327..41751)
                     /gene="degP"
                     /locus_tag="L2X49_000235"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBN4795387.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            complement(41881..43398)
                     /gene="dgt"
                     /locus_tag="L2X49_000236"
     CDS             complement(41881..43398)
                     /gene="dgt"
                     /locus_tag="L2X49_000236"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBN4795388.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCYFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            43482..44180
                     /gene="mtnN"
                     /locus_tag="L2X49_000237"
     CDS             43482..44180
                     /gene="mtnN"
                     /locus_tag="L2X49_000237"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBN4795389.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            44173..44973
                     /gene="btuF"
                     /locus_tag="L2X49_000238"
     CDS             44173..44973
                     /gene="btuF"
                     /locus_tag="L2X49_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBN4795390.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            45011..45634
                     /locus_tag="L2X49_000239"
     CDS             45011..45634
                     /locus_tag="L2X49_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBN4795391.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(45681..46025)
                     /gene="erpA"
                     /locus_tag="L2X49_000240"
     CDS             complement(45681..46025)
                     /gene="erpA"
                     /locus_tag="L2X49_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBN4795392.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEDDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(46018..46083)
                     /locus_tag="L2X49_000241"
     CDS             complement(46018..46083)
                     /locus_tag="L2X49_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795393.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(46107..47528)
                     /gene="clcA"
                     /locus_tag="L2X49_000242"
     CDS             complement(46107..47528)
                     /gene="clcA"
                     /locus_tag="L2X49_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBN4795394.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            47753..49033
                     /gene="hemL"
                     /locus_tag="L2X49_000243"
     CDS             47753..49033
                     /gene="hemL"
                     /locus_tag="L2X49_000243"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBN4795395.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(49068..51050)
                     /gene="fhuB"
                     /locus_tag="L2X49_000244"
     CDS             complement(49068..51050)
                     /gene="fhuB"
                     /locus_tag="L2X49_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBN4795396.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALIFWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLIIAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(51047..51937)
                     /gene="fhuD"
                     /locus_tag="L2X49_000245"
     CDS             complement(51047..51937)
                     /gene="fhuD"
                     /locus_tag="L2X49_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBN4795397.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(51937..52734)
                     /gene="fhuC"
                     /locus_tag="L2X49_000246"
     CDS             complement(51937..52734)
                     /gene="fhuC"
                     /locus_tag="L2X49_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBN4795398.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPTEGEILLDTQPLESWSSKAFARKVAYLPQQLPPAEGLTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(52785..54974)
                     /gene="fhuA"
                     /locus_tag="L2X49_000247"
     CDS             complement(52785..54974)
                     /gene="fhuA"
                     /locus_tag="L2X49_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBN4795399.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIANDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(55194..57728)
                     /gene="mrcB"
                     /locus_tag="L2X49_000248"
     CDS             complement(55194..57728)
                     /gene="mrcB"
                     /locus_tag="L2X49_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBN4795400.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(57924..60353)
                     /gene="hrpB"
                     /locus_tag="L2X49_000249"
     CDS             complement(57924..60353)
                     /gene="hrpB"
                     /locus_tag="L2X49_000249"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBN4795401.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            60427..60957
                     /gene="thpR"
                     /locus_tag="L2X49_000250"
     CDS             60427..60957
                     /gene="thpR"
                     /locus_tag="L2X49_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBN4795402.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            60972..61676
                     /gene="sfsA"
                     /locus_tag="L2X49_000251"
     CDS             60972..61676
                     /gene="sfsA"
                     /locus_tag="L2X49_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBN4795403.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            61854..62309
                     /gene="dksA"
                     /locus_tag="L2X49_000252"
     CDS             61854..62309
                     /gene="dksA"
                     /locus_tag="L2X49_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBN4795404.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            62346..63272
                     /gene="gluQRS"
                     /locus_tag="L2X49_000253"
     CDS             62346..63272
                     /gene="gluQRS"
                     /locus_tag="L2X49_000253"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBN4795405.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            63311..64729
                     /gene="pcnB"
                     /locus_tag="L2X49_000254"
     CDS             63311..64729
                     /gene="pcnB"
                     /locus_tag="L2X49_000254"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBN4795406.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            64726..65205
                     /gene="folK"
                     /locus_tag="L2X49_000255"
     CDS             64726..65205
                     /gene="folK"
                     /locus_tag="L2X49_000255"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBN4795407.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            65575..66159
                     /locus_tag="L2X49_000256"
     CDS             65575..66159
                     /locus_tag="L2X49_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4795408.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            66264..67004
                     /locus_tag="L2X49_000257"
     CDS             66264..67004
                     /locus_tag="L2X49_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBN4795409.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            67039..69639
                     /gene="htrE"
                     /locus_tag="L2X49_000258"
     CDS             67039..69639
                     /gene="htrE"
                     /locus_tag="L2X49_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HBN4795410.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RLSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIE"
     gene            69656..70225
                     /locus_tag="L2X49_000259"
     CDS             69656..70225
                     /locus_tag="L2X49_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4795411.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSSISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            70240..70842
                     /gene="yadL"
                     /locus_tag="L2X49_000260"
     CDS             70240..70842
                     /gene="yadL"
                     /locus_tag="L2X49_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HBN4795412.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            70869..71465
                     /locus_tag="L2X49_000261"
     CDS             70869..71465
                     /locus_tag="L2X49_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4795413.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            71518..>72300
                     /locus_tag="L2X49_000262"
     CDS             71518..>72300
                     /locus_tag="L2X49_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HBN4795414.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIY
                     IGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQ
                     QIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTN"
BASE COUNT        17786 a        19341 c        17698 g        17475 t
ORIGIN      
        1 ctcttcaacc gaaccagctg tttgtgtgaa gtgattcaca tccgccgtgt cgatggaggc
       61 gcattatagg gagtcggctc aggaagacaa gcggaaaaat gcatttttat ttcaaccgct
      121 catcttttaa tcattgcgcc ggtttttgct gcttttttat cgcttgcggc aggtctgcca
      181 ggctatttaa cacccaatct gccgcgtttt ctgcttcagg tgtaataggt ttacccgtac
      241 gcaccagcac ttttgttccc acgttcgccg caaccgctgc ctgcatatct tctaatttat
      301 cgcccaccat ataagaagcg gccatatcaa tatgcaaata atcgcgtgct gacaaaagca
      361 tccccggatg tggtttgcgg caatcgcaga cttggcgaaa ctcttcaaca ctaccctgcg
      421 gatgatgcgg gcaataatag ataccatcca gatcgacatc tcggtccgcc agcgaccagt
      481 ccatccactc ggtcagcgtt tcaaactgtg cttcggtaaa tttaccgcga gcaatgccag
      541 actggttggt tactaccacc agcgcaaagc ccattttttt tagctcgcgc atggcgtcaa
      601 taacaccgtc gataaattca aagttgtcga tctcatggac atagccgtga tcgacattaa
      661 tggtgccatc acggtcaaga aaaattgcgg gtacgctctt tgccaccttt tatagctcct
      721 taataaggca tgtgacgcta gtatcgcatg tttcgacctg caagaaagtg ctcttcgcat
      781 aaacctgatt gatttagacg tctggatgcc ttaacatcca tttcattgac ggcgttgccc
      841 gtttcaggca ttcgagatgc cacgactaac ttaatgacga taataaataa tcaatgataa
      901 aactttcgaa tatcaccaaa gtgttccacc agggcacccg caccatccag gcgttgaaca
      961 acgtcagcct gcatgtgcca gctggacaaa tttatggcgt tatcggtgcc tcaggcgcgg
     1021 gtaagagtac gcttatacgt tgtgtaaacc tgctggagcg cccaaccgag ggtagcgtgc
     1081 tggtcgatgg ccaggaactg accacgctgt cagaatccga gttgaccaaa gctcgccgcc
     1141 agattggtat gattttccag cattttaacc tgctctcttc gcgtactgtt tttggcaacg
     1201 tggctctgcc gctggagctg gacaacacac cgaaagacga gatcaaacgt cgcgtgacgg
     1261 aattgctgtc attggttggt cttggcgata agcatgatag ctacccatcg aatctttccg
     1321 gtgggcagaa acagcgtgtg gcgattgccc gtgcgttagc cagcaatccc aaagtattgc
     1381 tgtgtgatga agccaccagc gcgctggacc cggcaacgac acgttctatt ctcgagctgc
     1441 tgaaagacat taaccgccgt ctgggtttga cgattctgtt gatcacccac gaaatggacg
     1501 ttgtaaagcg tatttgtgat tgcgtggcgg tcatcagcaa tggcgaactg atcgagcagg
     1561 acacggtaag tgaagtgttt tcgcatccga aaacgccgtt ggcgcagaag tttattcagt
     1621 cgaccctgca tctggatatc ccggaagatt accaggaacg tctgcaagcg gagccattca
     1681 ctgactgcgt gccgatgctg cgtctggagt ttaccggtca gtcggtcgat gccccactgc
     1741 tttctgaaac cgcgcgtcgt ttcaacgtca acaacaacat tattagcgcg cagatggatt
     1801 acgccggtgg cgttaagttc ggcatcatgc tgactgaaat gcacggcaca caacaagata
     1861 cgcaagccgc cattgcctgg ctgcaggaac accatgtaaa agtagaggta ctgggttatg
     1921 tctgagccga tgatgtggtt gctggttcgt ggcgtatggg aaacgctggc aatgaccttc
     1981 gtatccggat tttttggctt tgtgattggt ctgccggtcg gcgtcctgct ttatgttacg
     2041 cgcccggggc aaattattgc taacgcgaag ctgtatcgta ccgtttctgc gattgtgaac
     2101 atttttcgtt ccatcccgtt cattatcttg ctggtatgga tgattccgtt tacccgcgtt
     2161 attgtcggta catcgattgg tttgcaggca gcgattgttc cgttaaccgt tggtgcagca
     2221 ccgtttattg cccgtatggt cgagaacgct ctgctggaga tcccaaccgg gttaattgaa
     2281 gcttcccgcg caatgggtgc cacgccgatg cagatcgtcc gtaaggtgct gttaccggaa
     2341 gcactgccgg gtctggtgaa tgcggcaact atcaccctga ttaccctggt cggctattcc
     2401 gcgatgggtg gtgcagtcgg tgccggtggt ttaggtcaga ttggctatca gtatggctac
     2461 atcggctata acgcgacggt gatgaatacg gtactggtat tgctggtcat tctggtttat
     2521 ttaattcagt tcgcaggcga ccgcatcgtc cgggctgtca ctcgcaagta acgttcaaca
     2581 caacataaat aattgaagaa ggaataaggt atggcgttca aattcaaaac ctttgcggca
     2641 gtgggagccc tgattggatc actggcactg gtaggctgcg gtcaggatga aaaagatcca
     2701 aaccacatta aagtcggcgt gattgttggt gccgaacagc aggtagcaga agtcgcgcag
     2761 aaagttgcga aagacaaata tggcctggac gttgagctgg taaccttcaa cgactatgtt
     2821 ctgccaaacg aagcattgag caaaggcgat atcgacgcca acgccttcca gcataaaccg
     2881 taccttgatc agcaactgaa agatcgtggc tacaaactgg tcgcagtagg caacactttt
     2941 gtttatccga ttgctggtta ctccaagaaa atcaaatcac tggatgaact gcaggatggt
     3001 tcgcaggttg ccgtgccaaa cgatccaact aatcttggtc gttcactgct gctgctgcaa
     3061 aaagtgggct tgatcaaact gaaagatggc gttggcctgc tgccgaccgt tcttgatgtt
     3121 gttgagaacc caaaaaatct gaaaattgtt gagctggaag caccgcagct gcctcgatct
     3181 ctggacgacg cgcaaatcgc tctggcagtt atcaatacca cctatgccag ccagattggc
     3241 ctgactccag cgaaagacgg tatctttgtc gaagataaag actccccgta cgtaaacctg
     3301 atcgtaacgc gtgaagataa caaagacgcc gagaacgtga agaaattcgt ccaggcttat
     3361 cagtctgatg aagtttacga agcagcaaac aaagtgttta acggcggcgc tgttaaaggc
     3421 tggtaatttt taggctgttt ccacaatttg taatatcatt caggacgggc gcttgcccgt
     3481 cttgtcattt ttacaagctc ctgattcaat attgacgttt tgatcataca ttgaggaaat
     3541 actatgcgtg ctttaccgat ctgtttagta gcactcatgc taagcggctg ttccatgtta
     3601 agcagatccc ctgtcgaacc cgttcaaagc actgcacccc agccgaaagc ggagcctgca
     3661 aaaccgaaag cgccgcgcgc cacgccggtc cgaatttata ccaatgcaga agaattagtc
     3721 ggcaaaccgt tccgcgatct cggtgaagtc agtggcgatt cttgccaggc ctccaatcag
     3781 gactctccgc cgagcattcc aaccgcacgt aagcggatgc aaatcaacgc ctcgaaaatg
     3841 aaggccaatg cggtattact gcatagctgc gaagtcacca gcggtacgcc aggctgctat
     3901 cgtcaggctg tatgtatcgg ctctgcgctt aacattacgg cgaaatgagc agtttccagt
     3961 tcgagcaaat aggcgttatt cgctcgccct ataaagaaaa attcgccgtt ccgcgccagc
     4021 caggtctggt aaaaagcgcc aacggcgaac tgcatctcat tgctccctac aaccaggccg
     4081 acgccgttcg cggcctggaa gcgttcagcc atttatggat ccttttcgtc tttcatcaaa
     4141 cgatggaagg cggctggcgt ccgactgtgc gtccaccgcg cctcggcggt aacgccagaa
     4201 tgggggtttt cgcaacacgc tctactttcc gccctaaccc aattggtatg tcgctggtag
     4261 agctgaaaga ggttgtttgc cataaagaca gcgtgattct gaagctcggc agtctggatc
     4321 tggtcgatgg tacgccggtg gtggatatca aaccgtatct cccctttgcc gaatcgcttc
     4381 ccgatgccag tgccagctat gcgcaaagtg cgccagcggc agagatggcg gtgagtttta
     4441 ccgcagaggt cgaaaagcag cttttgacgc tggagaagcg ttatccgcag ttaacgctgt
     4501 ttatccgcga agtactggcg caggacccgc gcccggccta tcgtaaaggt gaggaaacgg
     4561 gcaaaaccta tgccgtctgg ctacatgatt ttaacgttcg ctggcgcgtc accgacgcag
     4621 gttttgaagt ctttgcgctg gaaccgcgtt aaattcacgc ccttctcttt tgacatttct
     4681 tttgcactgg taaactaaat cacttttttt tgtcccaggc tcgccttgag cctgttctac
     4741 cttccaactg gaaccgtaac aacatgcgta ctagccaata cctgctctcc actctcaagg
     4801 agacacctgc cgacgccgag gtgatcagcc atcagctgat gctgcgcgcc gggatgatcc
     4861 gcaagctggc ctccgggtta tatacctggc tgccgaccgg cgtgcgcgtt ctgaaaaaag
     4921 tcgaaaacat cgtgcgtgaa gagatgaaca acgccggtgc gatcgaggtg tcgatgccgg
     4981 tggttcagcc agccgatttg tggcaagaga gtggtcgttg ggaacagtac ggtccggaac
     5041 tgctgcgttt tgttgaccgt ggcgagcgtc cgttcgtact cggcccaact catgaagaag
     5101 ttatcactga cctgattcgt aacgagctta gctcttacaa acagctgccg ctgaacttct
     5161 atcagatcca gaccaagttc cgcgacgaag tgcgtccgcg tttcggcgtc atgcgttccc
     5221 gcgaattcct gatgaaagat gcttactctt tccatacttc tcaggaatcc ctgcaggaaa
     5281 cctacgatgc aatgtatgcg gcctacagca aaatcttcag ccgcatgggg ctggatttcc
     5341 gcgccgtaca ggccgacacc ggttctatcg gcggcagcgc ctctcacgaa ttccaggtgc
     5401 tggcgcagag cggtgaagac gatgtggtct tctccgacac ctctgactat gcagcgaaca
     5461 ttgaactggc agaagctatc gcgccgaaag aaccgcgcgc tgctgctacc caggaaatga
     5521 cgctggttga tacgccgaac gcgaaaacca tcgcggaact ggttgaacag ttcaatctgc
     5581 cgattgagaa aacggttaag actctgctgg ttaaagcggt tgaaggcagt agcttcccgc
     5641 tggttgcgct gctggtgcgc ggtgaccacg aactgaacga agttaaagca gaaaaactgc
     5701 cgcaggttgc cagcccgctg actttcgcga ccgaagaaga aattcgtgcc gtggttaacg
     5761 ccggtccggg ttcactgggt ccggtaaaca tgccgattcc ggtggtgatt gaccgtaccg
     5821 ttgcggcgat gagtgatttc gctgctggtg ctaacatcga tggtaaacac tacttcggca
     5881 tcaactggga tcgcgatgtc gctaccccgg aaattgctga tatccgcaac gtggtggcgg
     5941 gcgatccaag cccggatggc cagggtacgc tgctgatcaa acgtggtatc gaagttggtc
     6001 acatcttcca gctgggtacc aagtactccg aagcactgaa agcctccgta cagggtgaag
     6061 atggccgtaa ccaaatcctg acgatgggtt gctacggtat cggggtaacg cgtgtggtag
     6121 ctgcggcgat tgagcagaac tacgacgaac gcggcatcgt atggcctgac gctatcgcgc
     6181 cgttccaggt ggcgattctg ccgatgaaca tgcacaaatc cttccgcgta caagagcttg
     6241 ctgagaaact gtacagcgaa ctgcgtgcac aaggtatcga agtgctgctg gatgaccgaa
     6301 aagaacgtcc gggcgtgatg tttgccgata tggaactgat cggtattccg cacactattg
     6361 tgctgggcga ccgtaacctc gacaacgacg atatcgaata taaatatcgt cgcaacggcg
     6421 agaaacagtt aattaagact ggtgacatcg tcgaatatct ggtgaaacag attaaaggct
     6481 gatgccagaa agggtcctga atttcagggc ccttttttac atggattgta taaaatggat
     6541 aaaccaaagg cgtactgccg cctgtttctc cccagctttc ttttactttc ggcttgtacc
     6601 gttgatatca gccagccaga cccatcagcc acagcagtag atgccgaggc aaaaacatgg
     6661 gctgtcaaat tccagcatca aagctctttc accgaacaat caataaaaga aataacggca
     6721 cctgatctca aacccggcga tttgctgttc tcctcaagcc ttggggtaac ctcatttgga
     6781 atccgcgtct tcagcacttc ctctgtgagt cacgttgcaa tctttctggg tgataataac
     6841 gttgcagaag cgacaggcac tggcgtccag attgtttccc ttaaaaaagc gatgaagcat
     6901 agtgataagc ttttcgtctt acgagtcccg gatcttaccc cgcaacaagc cacagatatc
     6961 accgcgtttg ccaataaaat caaagatagc ggttataact atcgcggcat tgtcgaattt
     7021 attcccttta tggtgactcg ccagatgtgc tcactgaatc cgttctccga ggattttcgc
     7081 caaagtgcgt cagcggcctg gcgaaagcgc agttaagcag tgtgggcgaa ggagacaaaa
     7141 agtcgtggtt ttgttcggaa tttgtcacgg atgcctttgc caaagccggg catccactta
     7201 cactggcgca atcgggctgg attagccctg ccgatctgat gcatatgcgt attggtgatg
     7261 tctctgcgtt taagccagaa acgcagttac agtatgttgg gcacctgaag cctgggattt
     7321 atatcaaggc gggtcgcttt gttgggctga ctcagtgata aagccagcct cgtgcacaaa
     7381 atgccggatg cggcgtaaac gccttatccg gcctacaaaa tcgtgctaat tcaaaatgtt
     7441 gcattatatg ctgtaggcct gataagcgca gcgcatcagg caattttgcg tttgtttctg
     7501 ccttaagcag gtttactgcc ccaaactact gcaatcctta ttcgggtaaa atttacccgc
     7561 cgtatcgggc gctaatactt tggttggcgc accagtatcc ggattagcct caagcgtaaa
     7621 gtgaccttct actgacagta acaccggttt ttcactgtga ccgcgcgcag ccaggtagct
     7681 acgctccagc tctgcgttat tcgctaccat gaaacgtttt ccggtcgcgc aatcagtgaa
     7741 ggtcgccgca tcagccatat aaaaatacat gcctcgcagg gtcatcggcg tcataggtaa
     7801 actggattgt gccggttcca gcgtatagtt gaactgcgat tcaatcggat tgccttcacg
     7861 atcgagcatc tccagcgcat cgcctttcgc ccgataatat gacttttcac ctttgctgtc
     7921 ggttaatacc agcttgtcag cggttcgcgc ccatgtaccg taggaagcga aggaggaagg
     7981 ttcttcacga gcaccgagat aacgctcatt catcacccat gttccgtctt tttcgaggaa
     8041 cagagaggtt tcgattcctt cgcaatcagc acacggcagc acgccgcgcc aactttgcgg
     8101 catcggtttc agttcggcag cctgcgccgg agaaagcgca tcaacttcgg cccgattatt
     8161 acatcccatc agagtaaaga ggctgattac agccatcgct gtcactatcg cttttttcac
     8221 catctattcc ttctttttat tcccgaccgc tgcgtacttt gccgcgcatc gccttcacgc
     8281 ttgatttttg tgctttcgat gccagcctgc gctctttcga tgcacgggtg ggccgcgtgg
     8341 gtcgtcgggc tttttgttct gttgttaaat ctttaatcac tgccaccagc cgggctagcg
     8401 cggcttcgcg gttcagttcc tgactgcggt attcctgtgc cttaatgaca atcacgccat
     8461 cactgctgat caaatgatgg ctggcggcga gtagacgctc tttgtaatac tctggcaggc
     8521 tggacgcccg aatgtcaaaa cgcagatgaa tggccgttga ggtcttatta acatgctgcc
     8581 cgcccgcgcc ctgcgcacga atggcggtga tctcaagctc accatcggga atagcaacat
     8641 gtcgggaaat cacaatcatg aaggttgttg ccaggcggtt aagttaactt ccagattatt
     8701 cttatcgtcc gataaccaga tcacgccatc ctgaatcgtt gcctgcagcg tcatggtacg
     8761 atcggcaaag gcgcttactt tcgccagttg ttcatcgtcc agataccaga cggaaagatt
     8821 ggcaaactgc acacatttgc tctgattttg ctgccaccag atttgcgccg cccgactatt
     8881 ataggtaaac agcaccactt ctgcggcctg ggtgcaggct ttcttaatcc gccgctcatc
     8941 cggcagcccc agctcaatcc acaaatcaat gcccaggtga tcgttacgca gccacgcttc
     9001 cggctcatca tcggcacaca aaccacgggt aaattgcaga cgttcatcag catatttcag
     9061 ccacgccagc aagcgcagca tcatacgctc ctgggtttct gaagggtggc gcgccagcgt
     9121 cagagaggca tcgagaaact ggttgcggtc gagatcggcc acattaaccg tcgctttata
     9181 aattgtcgct ttaagcacca taagaaaact cctttcgaat caggaggcca ttgtaacgga
     9241 atacgtgcag ttgcggcgct gatattgcag aaatgagtgt ggtatagtca ccttgcgaaa
     9301 cagagtttat cttcgtaggc ttagacttgc atccacggtt aagtcagagt gctgacagga
     9361 gggcatgtgg aaaaatattg tgagttaata cgcaagcggt acgcggaaat cgccagcgga
     9421 gacttaggat acgttccgga cgcgctgggc tgcgttttga aagtgctgaa tgaaatggct
     9481 gccgacgacg ccctttctga ggctgtcagg gaaaaagcag catacgctgc ggcaaactta
     9541 ctggtgagcg attatgtcaa tgaatgatac gtatcaacca atcaattgtg atgattacga
     9601 taatcttgag ctcgcctgcc agcatcattt aatgctgaca cttgagctga aagatggcga
     9661 aaaattgcag gcaaaagcca gcgatttagt ctcccgcaaa aatgtggagt atctggtcgt
     9721 cgaggccgct ggcgaaaccc gtgagctacg tctggataaa attaccagct ttagccaccc
     9781 ggaaatcggt acggtggtgg tgagcgagtc ctgattgact ttcgccggat gcgacgtttg
     9841 atgcgtctta tccggctttc acttaactaa gcgttttctg ccagacaaaa cttacgccat
     9901 tctcaccttc agccacacct tcttgcgtca caaatacccc tgccgccgca atcagcgttt
     9961 cgccataaaa cagcagtggc gtggtgtcac gtagccacgg cggcacgccc agctcttgcc
    10021 agattttctt tagcttacgt ccgccgttac gcccgacaat atgcagcaat cctggcgctt
    10081 tgaaacgcac gctgaccgct tcgtctgcac gcggagggcg aatatcgcct cccgcagtaa
    10141 gctgtacact tcccagcccc gccggtaatt ccagcggttg tagccacgtc tgccacggca
    10201 caatggtttc gctttgccca gtgacggagt taatccacca cagttgcgac tgatagcgcc
    10261 gtatttcaaa cgcgccaaaa cgtaaacagg gggaggcatc ttcccgcgcc agcgccactt
    10321 cctgccagat ccgcaccagc gcgtcgcggg aaggcatcgg tgcattctgc cctgccagcc
    10381 agcggcggat aatcgccgcg cgacgggcat cactcatcgc tagcattggc gcaatctgca
    10441 gcgtcccctg cgacgtttgg cagtgtgcta aatcatccgc cagcagttca tccaacagac
    10501 tctcttgttc agcacaaagt gcggcgctgc gggccgttgc ttcggcaaaa tgcggccaac
    10561 gctgctgcaa taacggcact acgcgcaggc gcagaaagtt acgatcgtat gagtcgtcct
    10621 gattactttc gtcttcaatc cagcgtaaac catgcgccaa tgcccactgc gccagttccc
    10681 cccgcgtgcg ggcaagcaac gggcggataa gccgtgttcc ggcaaactcc gagacttccg
    10741 ccatagccga aagcccggca gggccgctac cgcgttttag cgccagcaga aaggtttcac
    10801 attgatcgtc gagatgttgc gcggtgacca gcacttcgcc gggcaatagg gtacgggcaa
    10861 atgcctgata acgcgcctgc cgcgcttggg cctcaatgcc cagtccttct tgcgcaagtt
    10921 gcacgcgctc gaccaccagc ggcacctgcc actgttggca gatgttctcg caatgcctaa
    10981 cccaggcatc ggcattggca cttaaaccgt gatgcacatg gatagcgcgt agagtgacgc
    11041 ccggattttc cgtccgccac tgcaccaact gatgcagcag aacggtggag tcaagcccgc
    11101 cgctaaaggc caccagaatc tggcgtaagg tgagaagttg tctattgagc gtgagtgtca
    11161 tgatggtgcg attttacagt agcaatgccc ggcacgttac cgggcaaatg cggcgatgac
    11221 aagccttact gctcatacag ttccaacggc agcccgtccg gatcgttaaa gaacgtgaag
    11281 cgtttttgcg tgtatggatc gacacggatg gcttcacact tcacgttatg gctttcaagg
    11341 tgcgccactg ccgcatcgat atcatcaacg ctaaaagcca gatgacgcag gccgcaagct
    11401 tccggtcggc tggggcgctc cggcgggaac ggaaaagaga aaagctcaat cacatattgc
    11461 ccattaagcg ccaaatcccc tttccacgag tcgcgcgctt cgcgatagac ttcgctttgc
    11521 agcgtgaaac caagaatatc gcagtagaaa gctttgctca ccgcataatc cgtcgcaata
    11581 atcgcaatat ggtgaacctg ttttaaaccc agcataacgt ctcctttatt tgttaacagc
    11641 acgttactcg cccggaagcc gctctggcaa gttatcccgc catttttagg actcgtacgc
    11701 ggtaaacgcc gtcttcgtcc tgtttcgcgc cgtgaatatc cgtttcaaaa ccggggtaat
    11761 gttgccctac ggaacaaagc atcagtagaa aatcgagtac cgtgcggctc tctttggtca
    11821 gcatttctcc tggcatcaac agcggtacgc ccggtggata aggcaggatc atatttgccg
    11881 atactctacc gaccagttgt tccagcgcaa tggtttctac ttcaccttta atctgtctct
    11941 gccatgcctg atgtggcgtc atgatcattt ccggcaaagt atcgaatgcc cgcaacatca
    12001 aaccgggaag atcgtgttta cgaatcagct tatggatccc ttgcgccaga tcctgaatac
    12061 gcatattgcg gtagaaatcg ggatcttcag catagagatc gggtagcata tttttgatcc
    12121 gcaggttgag atcgtaagag cgtttaaatt ccgtcaaccc acgcaataat cccattgctt
    12181 tggttttatc gataccaata ctaaagagaa acagcaggtt ataagggccg gttttctcta
    12241 ctacgatccc acgttcgtcg aggaattttg ccaccagcgc tgccgggatc ccctcctcgc
    12301 tcatattgcc ctgttcgtcc atccccggtg tcaaaatagt gactttaacc ggatcgagaa
    12361 acatatgatc ggcatccgca tcgttaaagc cgtgccactg ttcgcctggc gcaacgggcc
    12421 agcattcggc ttcatccacc tgcggcggtt gccagatatc gaaaaaccaa ccgtcagact
    12481 cttcccgcaa ccgctggacc tctttgcgaa aatgcagcgc ccgctccacc gaacggttaa
    12541 tcagccgttt gcccggatta ccacgcagca tcgccgccgc cgtctcaacc gaagcaacaa
    12601 tgggataact gggcgaggtg gtggtatgca tcataaaggc ttcgttaaac gcctcttcgt
    12661 catactcgcc tttaatgtgg atcagcgaag cctgcgataa cgccgccagc attttgtggg
    12721 tcgattgcgt ttcgaagatc acttttcccg caacacgctc gccgctcata ccacttttcc
    12781 cctggtagat cggatgaaaa tgggtgtacg gcacccaggc ggaatcgaag tggatcgacg
    12841 ggacatccag cgtctgtttg atccagtcgg tgttgtagag caagccatca taggtggagt
    12901 tggtaatcac cgcatgaacc ggccattgtg cctgcgtggt ggcagcgact ttctcttcga
    12961 tgctgtcgcg agtaaattca cggcgcggga tcccaccaag aatccccaac gcattacgcg
    13021 tcggtttcag ccagactggc actacatcgt tcatcatcaa cagatgcgcc agcgatttat
    13081 gacaattgcg gtcgatcaac agcgtactgc cggatggcgc ggcgtacata cccacaattt
    13141 tgttcgacgt cgatgttccg ttggtaacga tataactctg ttccgcgcca aaagtccgcg
    13201 cgatgtactc ttccgcttcc aggtgtggcc cggtgtggtc gagcaacgaa ccaagctcgg
    13261 tgaccgaaat agagacatca gccttgaggg tattcccgcc gaaaaaatca taaaacaggc
    13321 agccaaccgg gcttttttga tatgcggtgc cgcccatatg ccccggcgta caaaaggtgt
    13381 acttccgctc tttaacgtag gtaaacaagg ctttcgtgaa cggcggtgta atgttatcaa
    13441 gatattcgtc ggtgtactga cgcatacgaa tggcgatatc ttccgcctgc cccagcgcat
    13501 attcaaaaaa ccagagcgcc atccgcatat cctgcacgct gacatccatc gtcgagtggg
    13561 tgttgatgaa ggcataaagc gggagatatt cattaagttg attgatatcg ctgcataaat
    13621 cgagactgta ctcatcccag tcaaaaatca cgccgcaaat tcgagggtta tgttcgataa
    13681 atttcagcaa atcaacgctg ttttgtggcc agataatctg aaagccttgc gccaccagcg
    13741 ccgactccag ttctttgatg ggctcatctt tataaaagac gccatgcggt cccataatgg
    13801 caatgatgtt catgtgttcc tcctggaaaa tccttcctta atcatagcct gctcaaaccg
    13861 tggcgataaa aaagggccac cgaagtgacc ctttttcaga acttttgcga attacgcgta
    13921 accgtagctc atcaggcgct gataacgacg attttttaaa tcttcagtgc ttaacacatc
    13981 gagatcggcc agatccgcca gcagttgcgc tttcaacgat gccgccatcg cttccgggtt
    14041 acggtgagcg ccgcccagcg gttccgggat gatggagtcg atcagtttca gctctttcag
    14101 acgcggagcg atgataccca tcgcttcagc cgccagcggc gctttgtcgg cgctcttcca
    14161 cagaatggac gcacaacctt ccggcgagat aacggaatag gtgctgtatt gcagcatatt
    14221 cactttatcg cccacgccaa tcgccagcgc accgccagaa ccaccttcac cgataaccgt
    14281 acaaactacc ggtacgccga ggcgagacat ttcacgcagg ttgcgtgcaa tggcttcaga
    14341 ctgaccacgc tcttctgcgc ccacgccagg ataagccccc ggggtgtcga taaaggtgat
    14401 gataggcatc ttaaagcgtt cagccatttg catcagacgc agtgctttgc ggtaaccttc
    14461 tggcgctggc ataccaaagt tacggcgaat tttttctttg gtttcacgac ctttttgatg
    14521 accaatgatc atcaccggac gaccgtcaag acgggcgata ccaccgacga tagctttatc
    14581 gtctgcatac gcgcggtcgc cagccagttc gtcaaattca tcaaatgcca ggcgaacgta
    14641 atccagggta taaggacgct gtggatggcg tgccagttgc gcaatctgcc atgcaccgag
    14701 atcggcgaag attttacgtg tcagttctac gcttttttca cgcagacgat gcacttcttc
    14761 atcgatgtta atatccagtt tctcatcctg acggctaacc gcagtcagag aatcgatttt
    14821 cgcttccagc tctgcaatcg gctgttcaaa atcaaggaaa ttcagactca tagtattcct
    14881 gtattagtca aactccagtt ccacctgctc cgaaccaatg aggccacgga gatcgtttaa
    14941 taaacgatcg ctcggagaga cacgccacgt cgcgccaaaa cgcaaccgcg cgcgtgcatc
    15001 cgccctctga tagtagagat gtactggaat tgtcccagag cggtggggtt ccagagactg
    15061 acggagtcgg tttaaaagct ggtcatcaat ttgcctgtcc gtcagcgaga tagcaagccc
    15121 gcgagcatat ttttcccggg cttcgtcaat atccatcact tcgcgagcgg tcattttaag
    15181 cccaccgctg aagtcatcaa agctgacctg tccgctgacg ataagtatgc ggtctttttc
    15241 cagcaattgc tggtatttat ccagggcgtc agtaaacaac atcacttcca gccgcccgga
    15301 acggtcatcc agcgtgcaga taccgatacg attgccgcgc ttggtgacca taacccgcgc
    15361 ggcaacaacg agccccgcag ccgtgatgac tttaccacgt tctgtcgggt gcatgtcttt
    15421 cagccttacg cctccgacat aacgctcaat ctcttttaaa tactggttga tagggtgtcc
    15481 ggtcaggtac aggcctaacg tttcacgttc cccatctaat accacctgct ccggccacgg
    15541 ttggcagctg gcgtaggatt gttcaatttg ttccggctct tcggccagca cgccgaacat
    15601 atcggcctga ccgatagctt ccgctttcgc gtgttgatct gccgctttta acgcatcgcc
    15661 cagcgagttc atcagtgccg cgcggtgtgg tccaagacgg tcaaacgccc cggacatgat
    15721 cagtttttcc agcacccgcc ggtttaactt tttggtgtcg gttcgggcgc agagatcaaa
    15781 cagttcgcgg aagtagccgc ctttattacg ggcttcgatg atggcctcaa tcggaccttc
    15841 accgacccct ttgatcgcgc caataccata cacgatttcg ccgtcgtcgt tgacgtggaa
    15901 atggtaaaga ccggagttta tatctggtgg caggattttc agccccatcc gccagcactc
    15961 atccaccagg cccaccacct tctcggtgtt gtccatatcg gcggtcatta ccgccgccat
    16021 aaactccgcc ggatagtgcg ctttcagcca taacgtttga tatgacacca aagcataggc
    16081 cgcagagtgc gatttgttaa atccgtaacc cgcgaatttc tccaccaggt cgaagatttt
    16141 catcgccagt tcggcgttga ttccgttctt ttctgcacct tcagcaaata cagaacgctg
    16201 cttagccatc tcttccggct ttttcttacc catcgctcga cgcagcatat ccgcgccacc
    16261 gagggtataa ccagaaagca cctgggcaat ctgcatgacc tgttcctgat acaggataat
    16321 gccgtaggtt ggctccagta ccggtttcag gctttcatgc tgccactgta cgtctggata
    16381 ggagatttct tcgcgaccat gtttacggtc aataaagtta tccaccatcc ctgactgcaa
    16441 cggaccaggg cggaacagtg ccaccagtgc gatcatatct tcgaagcagt caggttgcag
    16501 acgcttgatc aggtccttca tgccgcgcga ttcaagctgg aataccgccg tggtttccga
    16561 gcgttgcagc atgtcgaagc ttttcttgtc atccagcggg atcgcggcga tatccagcgg
    16621 tggctcgcca ttcttcgccc gccgcttgtt gatcatctcc agcgcccagt tgatgatggt
    16681 gagcgtacgc aaaccaagga agtcgaactt caccagtccg gcgtattcaa cgtcgctttt
    16741 atcaaactgg gtgaccggat gtttgccctc ttcatcgcag taaagcggcg caaaatcggt
    16801 aattttggtc ggcgcgataa ccaccccacc ggcgtgctta ccggcgttac gggtgacccc
    16861 ttccagtttg cgcgccatgt cgatcagcgc cttaacttct tcatccgctt cgtagatttc
    16921 cggcagctgc ggctcggctt caaacgcttt cgccagcgtc atccccggat cgggcgggat
    16981 cagtttcgag atacgatcga caaagccgta cggatgcccc agcacgcggc ctacgtcgcg
    17041 gatcaccgct ttcgccgcca ttgtaccgaa ggtgatgatc tgcgataccg catcacgacc
    17101 gtacatgtcc gctacgtgct cgataacctg gtcgcgtttc tccatacaga agtcaacgtc
    17161 gaagtcaggc atggagacac gttccgggtt aaggaaacgt tcgaacagca ggtcaaattc
    17221 cagcggatcg aggtcggtga ttttcagcgc gtaggccacc agtgaacccg caccggagcc
    17281 acggcctggc cctaccggta cgccgttatc tttcgaccac tggataaatt ccataacgat
    17341 gaggaagtag cccgggaagc ccatctggtt gataacctga agttcagtct ccagacgttc
    17401 gtcatattcc gggcggcgtt taagacgttc ttcctcatca gggaataaaa aggccagacg
    17461 ctcttccagg ccttcttttg cacgcttgac cagataatct tcggtgctca tgtccccggt
    17521 cgggaactgc ggcaggaagt attcaccaag acgcacagtc acgttacagc gtttggcgat
    17581 ctcaacggtg ttggcaagag cttctgggat gtcggcaaac agctcgcaca tctcctcttc
    17641 gctacgcata tattgctgcg gcgaatagtt acgcgggcgt ttaggatcgt cgagggtaaa
    17701 gccgtcgtgg atcgcgacgc ggatttcgtg tgcgtcaaag tcgctgctgt cgataaagcg
    17761 cacgtcgttg gtcgccacga cgggcaaacc gcgcgcttcc gccagttcca ccgccgcgtg
    17821 cagatagctt tcttcatccg gcctgccggt gcggatcagc tcgagaaaat agcgatccgg
    17881 gaagtgttct tcataaaacg cgacacactc atctaccagc gcgctgttac cacgcaaaag
    17941 actgcgtccg acgtcgccca tgcgcccgcc ggaaagaagg atcaaccctt catttaattc
    18001 gataagccag tcgcgatcga tgatcggccc agcggcaccg tacccacgct gatacgcttt
    18061 tgagatcagc aacgtcagat tctgatagcc ggtattgttc gccgccagta ccgtcaggtg
    18121 ggttaactca tcacccagca ggtcgcactg gacgttaaaa tctgccccga cgataggctt
    18181 aatccctgcg ccatgtcccg ctccgtagaa cttcaccaga ccgcaaaggt tggtgaaatc
    18241 ggtgatcgcc agtgctggca tacccaacgc cgccgccttt tttaccagcg gtgcggtttt
    18301 ggccaggcca tcgatcatcg agtagtcgct gtgcacccgc aggtgtacga aacgtggttc
    18361 agacatcttc agattccggt ttacttaatc tcgacaagaa tcaggacgca agtcccagtg
    18421 ctcgtttgac aggcccaaag ctgcgacgat gatgttcagt cgcgccatat tcagccagtt
    18481 tttccagatg aaaagcggtt ggataccctt tgtgttgggc aaaaccatat tgcgggaaaa
    18541 caatatccag cgccgccatt tcggcgtcac gcgtcacttt cgccaggata gacgcggcac
    18601 tgatttccgg tacgcggcta tcgcctttca ccacagccat cgcaggcatc ggtaatttcg
    18661 ggcagcggtt accatcaatc aacacatatt ccggcgcaat atgcagccca gcgacggcac
    18721 gctgcatcgc cagcatggtc gcatgaagaa tgttcagctc gtcgatttcg tggggttccg
    18781 cgcggcccag actccagctc aacgctttct ctttgatctc ttcacagagc gccagacggc
    18841 gtttttcgct cagctttttg gaatcattca gcccggcaat cgggcgcgcc gggtcgagga
    18901 tcaccgcagc ggtgacgacc gcgccaacta acggcccgcg tccgacttca tccacacccg
    18961 caaccaggtg cgtgtgcgga taaacaaatt cgatcattgt gctaactcca gaacggcttg
    19021 tgccgcctgc tcatcggcat tgcagcggat ctgctgatgc agttcacgga aggtatcgtg
    19081 catcgcgtgg ctggttttcc cgttcgccaa cagcggtaac agcgccgcag ccagtttttg
    19141 cggctcacac tcttcctgca ataattcttt gactaactct ctacccgcca gcagatttgg
    19201 cagcgagaca taatcagttt tcaccagccg cttcgccaac cagaaggtaa aaggcttcat
    19261 gcgatatccc accaccatcg ggcatttcgc cagcatacac tccagggctg ccgtacccga
    19321 cgccagtagc gccgcatcgc tggcgaccat cgcctcacgg cccatcccat ccagcaaatg
    19381 aactgaaagg tctggcgcga cttcagcttt gatgcgttca aactgctcgc ggcgtttggc
    19441 attcaccagt ggcaccacga tttcgagatc cggatatgtc tggcgcaaaa gctgggccgt
    19501 tttcaggaaa tcggcactaa gcatttcaac ttctgcacca cggctccctg gtagcaacgc
    19561 caggcagtgg gcatcgtgag ggatccccag cacatcacgg gcggcatttt tatctggatc
    19621 taacggcatg gcatcagcca tggtatgacc gataaagcgg cacggtacat tgtatttgtc
    19681 ataaaacgct ttttcgaaag gcagaaatgc gagcaccaga tcggtggctc tgcctatttt
    19741 gaaaacgcgt ttctgtcgcc acgcccagac ggatggactg acgtaatgaa tggttttgat
    19801 accctgcttt ttaaggttac cttcgagggt aatattgaag tcaggcgcat caatgccaac
    19861 aaaaacatct ggctttagtt caccaaaacg ctttgtcaga tcggcacgaa tatgcagtaa
    19921 gcgacgcaga cgaccgagca cttcaacaat gcccatcacc gccagttctt ccatttcgta
    19981 ccaggcttcg cagccttcag cctgcattcg tggcccggca acgccaacaa agcgggcgtt
    20041 gggcacacgt tctttcagag cgcggattaa accggccccc aggatatcgc cggaggtttc
    20101 tccggcgacc agggcaatcg ttaatggacg ctgtttagtc attaacgaat cagaccgcgc
    20161 gttgagcgtg caaagaaatc ggtaaaagct ttcacttccg gatacgtttc cgccagttca
    20221 gcaatttccg gtttcacttc atcgagcgtt ttaccgctgc gataaatcag cttatacgca
    20281 ttgcggatag cggtaatcgc ctcgcggctg aatccgcggc gcttcagccc ttcgatattg
    20341 acaccgaacg gcgttgcgtg gttaccctgc gcaatgacat aaggagggac gtcctgcgcc
    20401 acaccggagc aaccgccaac catcacatgc gcaccaatga tgcagaactg atggactgcg
    20461 gtcatgccgc cgatgatcgc gaagtcgtct accgatacgt gacccgccag cgttgcgttg
    20521 ttggcgagaa tacagcggtt acctaccgta caatcgtgcg caatgtgcgc gttgatcatc
    20581 agtaagttgt cgctgcccac cttcgtcaat ccaccgccct ggactgtgcc acgatgaatg
    20641 gtgacgcttt cgcgaatgcg gttacgatcg ccgatttcca cacgggtcgg ttcgccagca
    20701 tatttcagat cctggttaac ttcaccgatg gaggcgaact gataaatctc attatcgcgg
    20761 ccaattttag tatggccatt cacgacaacg tgagatttca gtacggtacc ctcaccaatt
    20821 tcgacatggg gtccaacgat acaaaaagga ccaatgtgtg cgttcgcgcc aatcgacgcg
    20881 ccctcttcca caatggcggt tggatgcaca aaggcggatt tatcaatcac gtatcaggcc
    20941 tcccggctac gagcacacat catcgttgct tcgcaaacta ctttaccgtc gaccagagca
    21001 acccctttaa aacgggtcag gccgcggcgc gttttttcga aagtgacttc catgatcatt
    21061 tgatcgccag gcacgaccgg gcgcttgaag cgcgcttcgt caataccagc gaaatagtac
    21121 agctcacccg gttccagttt tcctacgctt ttaaacgcca gaatacctgt tgcctgtgcc
    21181 attgcttcca gaatcagcac acccgggaaa atcggttttc cagggaaatg gccctggaag
    21241 aatggctcat tgacagagac attttttact gcgcgcagaa aacgaccttc ttcaaaatcc
    21301 agcacgcgat ccaccagtaa gaaagggaaa cggtgcggca gaagttctaa aatctcttca
    21361 atctgcagag tatgagtgtt agtagtcaag atactcttcc tgtcaaaata taagaaacga
    21421 caataataac acggcctgcc gcaatcgtaa gaatgagaca ggccgtaaag tttggcgaac
    21481 aaaagatgga acgttagtct tgttgattaa ccttgcgctc aagcgatttc agacgcttgc
    21541 tcatgtcatc aatgttcatc accagtgcag cggttttgcg ccagactttg ttgggttgca
    21601 gcggaatgcc tgaggaatag acgcctggtt cagtgatggg acgcatcacc atacccatgc
    21661 ccgtaaccgt cactttgtcg catatttcca tatgcccgtt gattacgctg gctccgccga
    21721 tcatgcagta acgaccaatt ttcaggctgc ccgccataat gacgccaccg gcaaccgccg
    21781 tattgtcgcc aatcacgacg ttatgtgcaa tctggcactg gttatcaatg atcacgccat
    21841 tgccaataat agtgtcatcc agcgcgccgc gatcgatggt tgtgcaggca ccgatctcca
    21901 cgcgatcgcc aataattacg cgaccaatct gtgggatctt cacccagtta ccacgatcgt
    21961 tggcataacc aaagccgtct gcgcctacca ctgttccgga ctggatcagg caattctgac
    22021 cgatctggat ctcatggtaa atggttacgt tcgcccagag acgcgaacct gcaccgattt
    22081 tgctgttttt acctacgaag caaccggcac cgataatcac gttatcgccc agttcaacgc
    22141 cggactcaat caccgcgtta gcgccaatcg atacgttgtt acccagcttc gccgtcgcgt
    22201 cgatcaccgc actgggtgca atgttctgcg cgggctgcgg cgtggtatct aaaatttgcg
    22261 ccatgcgcgc gtaagtcagg tagggattct tcactaccag cgcggcactt ttcgcgaaag
    22321 gaagatcgtc ctgggtcatg acaaccgcgg acgcctggca caagcctaaa tgctcacggt
    22381 attttgggtt aaccatgaac gtaatgtgac ctgtttgtgc agattgcatg gacgcaacgc
    22441 cggtgatgac gatatcgcca tcaccgtgta gttctgcatc caactgctgc gctaaatcag
    22501 ccagtcgaat tgaaggcatt acttatttaa cctgtttcag tacgtcggca gtgatgtctt
    22561 ttacatcgct gctgttgtaa gcaacggcgt ttgcatcaac aaccagatcg atatcctggc
    22621 tgttggcaac ggatttcaca gcagtctgga tacgagtaac cagtttgccg cgttcttcgt
    22681 tggaacgacg tgcgcgatcc tgctcaaaag cctgcgcttt ctgagcaaaa gtctggcgct
    22741 gagccatcac gtctttttcc agcttagtgc gatcgctgcc cgctttcatg gactgcagct
    22801 ttttcatttt agcctgcaga tcggtttcca tacgctgcag ttcgctggca cggcctttga
    22861 actcattttc cagcgtgtta gaaacaccgg ttttctgcgc tacctgctgg aacaggctgc
    22921 ccatgttgac gattgcaatt ttgtcagccg cctgagcaga agttgccagt gctaaaccga
    22981 gacctgcagc taataaccac tttttcacaa taaactcctt accatcccat ttgcaccgga
    23041 gggtgcagtt ctttgcgtgg cccggcgatc ttatattgat cgcctaaagt catcgctaca
    23101 ctaccactac attcctttgt ggagaacact taccaggttt taccgatgtt aaactggaac
    23161 tgttctgcct tgtctccatc gtactttttg aacggctggg cgtaggagaa caccaacggc
    23221 cccaatgggg acatccattg taatgcgata cccgcagaca tacggatatt gcttggatca
    23281 ctatagtccg gatatccaga atattggctg gaatcccagt ttgtatccca aacggtaccc
    23341 atatcccaga agaaggaagt acgaaccgag ttagcatact tatcgctaat aaacggcgtc
    23401 ggggtgatga actcgaggct ggcaaccgcc atggcgttac cgcctacagc atcatccgat
    23461 ttacacaggt ctttcgcgcc gtcctgagtc gcacattcgt aatcatagtc cggatcataa
    23521 ttactggcct gatgcgggaa gtaaactgct ttcggaccaa ttgtattgga ctggaagcca
    23581 cgcacggtgc tggaaccacc ggcatagaag ttctcgtaga acggcatctc tttgccgcct
    23641 aaaccatcac cataacccca gcgggtacgc cccagaacaa cccatttgtg atcgtcatcg
    23701 atcggcacat aagtcgccgt gtctaacgtc actttgtagt attcgttatc cgatccagga
    23761 atggtcactt taccggtcag gttgacacgt gaaccatctg tcgggaagta accacggtca
    23821 agcttgttat aggtccaacc atagttgaac gtgaagtcgt ccgttttgaa gctattatcc
    23881 tgatcagagg tgctcggatg ttcacccata gagtacagat aacgccacat cgcaacctga
    23941 ggctgcatgt tggacaggga gttatgtaca taacccagac ctgcacgcag cgagttatat
    24001 tcgttgatcg ggaagcccaa cgtcacgtct gtaccataac tcttgttggt atagtcggac
    24061 aggtcggcgt catctgcctg gaagtcatta tagaagagac gaccaccgag gcttacacca
    24121 tctacagtga agtacgggtt gattaccgac agttcagcat aggtctggta atcgtttttg
    24181 gtcccgttga taccaacagc ataacctgta cctaaccagt tatcctgctg cacaccagcc
    24241 tggaagctca cgccactttc agtaccgtaa ccaataccaa agttgaagct accggtgttg
    24301 cgctctttta ccttgtagac gacatctacc tggtccgggc tacccggaac acgttgggta
    24361 tcggtatcga cagtttcaaa gaagcccaga cgattcagac gctccttacc ctgatcgacc
    24421 agatcgcttc ccagccatgc accttccatc tgacgcattt cgcgacgcag gacggcatct
    24481 ttcgaggtat cgttaccttc aaaacggatc ttacgcacgt agaaacggtt acccgcatca
    24541 acgttcacgc gtaatttaac ggttttgtcg gcatcgttaa tttcaggcat cgactgtacg
    24601 cgcggatagg cataaccata gcgaccgaga agctttttga tgtcatcttc catcttggtc
    24661 actttggtgc cgttatacag ctcacccggc tcgatcttag tcagctgctc aatttcagcg
    24721 gagtgcccgg caaggttgcc gctcacttca acgccagaaa gcttgtactg atcgccttcg
    24781 gtgatgttca ccgtgacgta aatacctttt ttatctggcg tcagactgac ctgggtagag
    24841 tcgatgttga aacgggcata accgcgatcc agatagtagc tgcgcagggt ttcaaggtcg
    24901 cccgccagtt tctgtttctg gtatttacga tcgccaacca cgttccacca cggcacttcg
    24961 tcacgcagtt ggaaatgaga gatcagttcg tcggtggtga aagcatggtt accaacaatg
    25021 ttaatttgct ggatttcagc tgacacacct tcctggaaca ccagttttag gtcaacacgg
    25081 ttgcgcggca gcggggtcac gacagctttt acgctggcgc tatatttacc gacgctgtag
    25141 tagaagtctt ccagaccttt ctcgatatcg gcaatggtgg tgcgatcgag ggattcgccc
    25201 acacgcacac cagaagcctc gaggttttgc ttcagcatgt catctttcac cgatttgtta
    25261 ccggagaaag taatgctggc aatggtcgga cgttctttta cctgaaccag aagggtatca
    25321 ccatcacgaa ggacgcgaac atcctcaaag ttgccggtag caaacagagc gcgaatggta
    25381 ttactgatat cttcatcatt aaccgtgtcg cctgtgcgca ccggcatact gaggagggcc
    25441 gcaccaacgg cgacacgctg aaggccttcg aaatgaatat ctttcactac gaacccttca
    25501 gcaccgtata cggtggcgct gctaaacagc agcgacgcta tgagcaactt tttcatcgcc
    25561 atcgttatta tgcgttcttc ctaactaact ctcataaccg agagaaatca ttgaaaagtg
    25621 caagccccat taacagcacc agcagaatcg agccaatgcg ataacaaaag tcttgaaccc
    25681 gctcggatac cggtccgccc ttgatctttt cgatcgcaag gaacagcaga tgccccccgt
    25741 caagtacggg caacggaaac aggttaatta tccctaagtt cacgctaata agcgcaagaa
    25801 acggcaggta ataaactacc ccgagttccg ctgtcatccc agcccccttg gcgatagaga
    25861 tcggcccact gaggttgttc agtttcacat caccggtgat caattttccc agcatactga
    25921 ccgtcagctt catcagctgc cacgttttgt ccgtggcttc gacgatggcg ttgaacggcc
    25981 catactggcg tacaacttta tactcatctg gcaaaggaat gactttcggc tcaataccga
    26041 caaaaccaat cgctttacca ttacccggtt tactctccgg gattaatgtc aaagacaagg
    26101 gacttccctg cctttcgatt tctaacgcta aggatttacc cgggttatcc cggacaagca
    26161 tcacaaaggt cacccactgc gttaagggct gaccatcgac tttaacgatc ctgtcgcctg
    26221 cttgcaaacc tgccttgctt gccgccgagt ttggctgcac attttccagt acaggttcaa
    26281 tttgcggccc acgaggacga atccccagcg aagataccgg atcttcttta tcaggctcaa
    26341 acgcccagtg acgtaaatcg agctttacat cccgccgttg gtcgctgcca aatggcgcta
    26401 ctgtaatggt ggtgctttca tcgccaattt tatcgaccaa ctgcaaacgc acggcatccc
    26461 aatcaggcgt ttcgatacca tctacggctt ttagttccgt acctggtgca atttgtgctt
    26521 ccgcagctat cgaattggct gctatttcgc caaccaccgg acgtacgcca ggcacaccaa
    26581 taataaaaac cagccagtag gcaaagatag caaaaatgaa gtttgcaacc ggacctgcgg
    26641 caataatcgc cgctcgttgg ccgacagatt tattattgaa ggcatggtgg cggagttccg
    26701 gaacgaccgg ttctgcgcgc tcatccagca ttttgacata accgcccaac gggatcaggg
    26761 cgataacata ttcggtgccg agcttatcag ttcgccgcca gagcgcctta ccaaacccta
    26821 ttgagaaacg ctcaacgcga acaccacaac gccgggcaac ccagaaatga ccaaattcat
    26881 gcacggtgat aagtacaccc agtgcaacga tgaacgaagc caaatcccag agaaaactca
    26941 gcatattacc ttccgttaaa gcgtcctgaa taccagtaac aacaagcaag caaagaccgg
    27001 taccgcagcc gtcaggctat caatacgatc taaaatacca ccgtgtcctg gaattaaatg
    27061 accgctgtcc ttaattcctg cttcacgctt aaacatactc tcggtcagat cgccgagcac
    27121 tgaggctaac gctgcgacaa tagagcaaat gagtaaggtg acgggagcga cgtcgagatt
    27181 cgcccacatg ccataacccc atgagattac cgctgcagta gcgagtccac cgataaagcc
    27241 ttgccaggtt ttacccggag aaaccttcgg tgccagctta tgtttaccaa acaatttgcc
    27301 aaacatatat gctccggagt cagcgcccca taccaggatc atgacataga gcagccatat
    27361 tgcgccactg taatgattct cgtcatagtg ccaggcccgt aacgccagca tgccccagaa
    27421 gaagggaaca atggttagca cgccaaaaat aaggcgcaat gttttagagt tacgccagat
    27481 tgctgcggaa cctgggtaaa acagcaccag caatagcgcg acaatccacc aacccagcga
    27541 agcccaaagt gagatttcaa ccagcggttg atgaatattt cggtgatatt ccggcaacag
    27601 aaaaagcatc agcgccaaca ataacccgca taacaccgcc aaccatactc gctgcgaacg
    27661 agtggtaaaa ccgctaagct gtccccattc ccacgctgcc agcatgcaga ccaccagcgt
    27721 tacaatggcg aaccccaccg gcggcaacag aaacaacgcc gcgatgacga cgggtattaa
    27781 cacaaaagca gatatcaggc gatacttcag caaaagcgac ccccatcagg ctgtttcatc
    27841 accgggctcg gtgccgccga aacgacgctc tcgattagca aaggcattta acgccccttc
    27901 aaagtcttgt tcatcgaaat cgggccagag aacatctgta aagtaaagtt cggcataggc
    27961 aatttgccaa agcaaaaagt tactaatgcg atgctccccc ccagtcctaa ttactaaatc
    28021 tacaggggcc agttcatgca tacagacatg ctggtttagc atctcttcat ctatctgatc
    28081 tggttgcagg tttccttgct gcaccttttc agccagttgc ctgactccct ggactatatc
    28141 ccaacgtcca ccgtagttcg ccgcaatatt cagcgtcaga ccggtattcc cggctgttag
    28201 cgcttcagat ttacgaatac gttcttgcaa acgcgagtta aagcgactgg tatcgccaat
    28261 aatacgcaga cgcacgttat gtcggtgcag actttttact tcgctatcga gcgcccacac
    28321 aaacagttcc attaacgcac tgacttcctg cgctggtcgg ttccagtttt cactactaaa
    28381 ggcatacagc gttaacgcct caataccgtt gttggccgca aaagagacag cccggcggac
    28441 ggattttgcc ccggctttat gcccaaaggc acgaatcttc ccttgctttt ttgcccagcg
    28501 accattgccg tccataatga tcgcaacatg acggcagcca tgcgctggca atttttcgct
    28561 aagtggttga gtagcagaca acatcacgcg ttttttattc cctgacaggg tttacggtac
    28621 tcaggaatac tgaagcctat acaaaaaaaa gccgtgtaaa accacggcat acctgacctt
    28681 ataaagccaa ctacttacga tcagatggcg cagactatat cactgaagcc ctacgctaac
    28741 aaatagcgcg actctctgta gccggattat cctcagcttg cgagacgcat cacctctttt
    28801 ctggcgactt cacgcgcgtt cgcatcaaca gataacacat cgtccacaca ttgtggttcg
    28861 cgcatatcca ttttttccag tacggataaa ttcaacgcag cgatatccgt aaagcggatt
    28921 tgttgcgcaa gaaaagcagc aacggtgatt tcgtttgcgg cattcaatgc tgtagtcgct
    28981 gcctggcctt gttcgaacgc ctccatcgcc agtttcaggc atggataacg atcataatcc
    29041 ggtgcggcaa atgtcaacgc acttagtttg caaaaatcga gcggcttcac gccagagttc
    29101 acgcgattcg gccatgccat ggtgtgggca attggcgtac gcatatccgg ttcccccagc
    29161 tgcgccagaa cactgccgtc ctgatagcgc accattgagt gaatcactga ctgcgggtga
    29221 atcagcacct ccatctggct ggcgctggcg ttaaacagcc aacgcgcttc aatgtattcc
    29281 agacctttgt tcatcatggt agccgaatcg acagaaattt tacgtcccat cgaccagttc
    29341 ggatgacggc aggcttgatc gggcgtcatt gttgccaaat cgcgcaatgg cgtctcacgg
    29401 aaagggccac cagacccggt aagtaaaatg gacaccacgc cattttgctc aaggtcagcg
    29461 tatcccagat tatgctggat aggttgcggt aaactctgaa aaatggcgtt atgttcgcta
    29521 tcgactggta acaattgcgc tttgctctgc tttacggcgt ccataaacag acgtccgcag
    29581 gtaaccagtg attctttatt ggccagcaaa atggttttac ccgcgcggat cgcagcaagc
    29641 gtaggtaaca gcccagcagc gccaacaatg gctgccatca cctgatcaac atcctcaagc
    29701 gctgccatat cgcaagcggc ttgttgccca cttaagactt cggtgcagct accctgttgc
    29761 tgtagcatcg ttttaagaag tttcgcactc gcttcatcgt ccattacggc atagcgggga
    29821 gagaattcca ggcactgttc taccatgcga gtgacatttt tgcctgccac cagcgcaact
    29881 acgcggaagt gttcgggatt atggcgcacc acgtccagcg tgctgcaacc aatcgagccg
    29941 gtcgagccca gaatggtgag ttgcttcatg aaacatccag agttgagaca gaataaaaag
    30001 caaaacgccg ccagccgatc cgcaaggatc tactgagcgg cgtttttgtc gttcaagaaa
    30061 tcagaactgc atcagttctg cttctttgtc tgccagcgcc gcttcaattt tcttgattgc
    30121 agcatcagtc agtttctgta catcgtcctg agaacggcga tcgtcgtctt cgctgatctc
    30181 tttatctttc aacagtgctt tcactttgtc gttcgcgtca cgacgcacgt tacgtactgc
    30241 aacacgcgct tgttctgctt caccacgaac gattttggtc agatctttac gacgttcttc
    30301 cgtcagcggc ggcagcggaa cacggatgtc gctacccgca gagttcgggt tcaggccaag
    30361 atcggacgcc ataatcgctt tttcaacggc cggagacatt gaacgatcaa acacgttgat
    30421 tttcagagta cgggaatctt ctaccgttac gcttgccagc tgacgcagcg gcgtcggcgt
    30481 gccgtaatat tccacgacaa tgccatccag caggctggga gaagcacgac ccgtgcgtat
    30541 tttgctgatt tgggttttga acgcttctac gcatttgtcc atgcgtactt cagcatcttt
    30601 tctgatatcg ctaatcacgt tacgaatcct tgaaaacttg tctcagtcag accagacagt
    30661 cacacacagt ggaagtgtgc taagtatagc cctgattaaa catattacgg gtaagatccc
    30721 ccgcgatact tacgcggaat cttaccctta tttatccatc acgggaatta ttccgtgatt
    30781 aaagtccctt ctttttcacc cattaccaca cggcgcagcg cacccggttt gttcatgttg
    30841 aaaacacgaa tcggtaattt atggtcacga gccagcgtga aggccgccag gtccatgact
    30901 ttcagctctt tttccagcac ttcgctgtaa gtcagttgct cgtacatggt tgcagttgga
    30961 tctttcgccg gatcagcagt aaacacgccg tcaactttgg ttgctttcag caccacatcg
    31021 gcttcaattt cgataccacg caggcaagct gctgagtcgg tggtaaagaa cgggttacct
    31081 gtaccggcgg agaggatcac cacacggttg ttgcgcaaca ggctgatagc ttctgcccag
    31141 ctgtagctgt cgcacacgcc attcaatgga atagcggaca tcagacgagc gttcacatag
    31201 gcgcggtgca gtgcatcacg cattgccagg ccgttcatta cggtcgccag catccccatg
    31261 tggtcgccca caacgcggtt catacccgct ttcgccagac cagcgccacg gaacaggtta
    31321 cccccaccaa tcaccacacc aacctgaata cccagttcaa ccagttcttt gatttcctga
    31381 gccatacgat ccagtatgct tgcatcaata ccgaagcctt cagtgccctg cagagcttcg
    31441 ccactcaact taagcagaat gcgtttatag acgggttttg cattggtagc catgtttctt
    31501 tcctgagact gtcaacgatt gaaatgaatt aatactagcg acattgtacg tcgcttttca
    31561 gcgcagcccc acaagggtta gctgaattta caagatgggc acaaaaagaa gccgccctca
    31621 ggcggctcct ttttgataat taagactgct tggacatcgc agcaacttct gctgcaaagt
    31681 cagtctcaac tttctcgatg ccttcaccca cttcgaagcg gatgaagcca gtcacttcag
    31741 cgttatgctc tttcagcagc tgaccaacag ttttgcttgg ttccataacg aacggctgac
    31801 cggtcagaga aacttcgccg gtgaatttct tcatgcggcc ttcaaccatt ttctctgcga
    31861 tttctttcgg cttaccagac tgcatcgcga tatccagctg tacctggtat tctttttcta
    31921 ccacttcagc ggatacgtct tctggtttga tgaattctgg cttgcttgca gcaacgtgca
    31981 tagcgatgtg tttaaccagc tcttcgtcag cgcctttagc agcaaccaga acgccgatac
    32041 gcgcaccgtg ctgataagaa cccagaacgt cgccttccag cgcagcaacg cggcgaatgt
    32101 tgatgttttc accaattttc gctaccagcg caacacgttc ttcttcgaac tgtgctttca
    32161 gaacttcaac gtcagtgatt ttgccagcaa cagctgcgtc cagaactttg tctgcgaacg
    32221 cctggaaacc agcgtctttt gcaacgaagt cagtctggca gttaacttcc agaatgatgc
    32281 cgtagttgcc gtcgattttg gttttgatca cgccgtcagc agcaacgttg cctgcttttt
    32341 tcgctgcttt aatagcaccg gacttacgca tgttttcgat tgccagctcg atgtcgccgt
    32401 tagcttcagt cagtgctttt ttgcaatcca tcatgcctgc gccagtacgc tcacgcagct
    32461 cttttaccag ggatgcggta atttcagcca ttctaaaatc ctcggagatg tgatctgccc
    32521 ggcattaaac cgcacagata taaaagtgaa aaagggggcc atatgcaggc cccctaacca
    32581 aacgtgtact acctggtcta taagggctct cccccttcat atttcgcgcc gcagatgcgt
    32641 tatcttcgct cgctcatccc ggtcacttac tgatgtaagc tcccgggaat tctcgagctt
    32701 gccgcctttc tgcaactcga actattttgg gggagttatc aagccttatt actcagcttc
    32761 tacgaagctt tcttccgcct gggaagccag atcctgagaa cggccttcac gtacggttgc
    32821 agcaacagcg cccaggtaca gggtcacagc acggattgcg tcgtcgttac ccgggataac
    32881 gaagtcaaca ccgtccggat cagagttggt atcaacgata gcaaataccg gaatacccag
    32941 gttgtttgct tctttgatag caatgtgttc gtggtcagca tcgattacaa acagagcgtc
    33001 cggcagaccg cccatgtctt tgataccgcc caggctgttt tccagtttct ccagctcacg
    33061 agtgcgcatc agcgcttctt tcttggtcag cttttcgaaa gtaccgtcct gagactgagt
    33121 ttccaggtct ttcagacgtt tgatggactg acgaacggtt ttccagttag tcagcatacc
    33181 gcccagccag cgatggttca cgaagaactg gtcgcagctc agagcagcgt ctttcaccgc
    33241 ttcgcttgca gcgcgtttag taccaacgaa aaggatttta cctttgcgag aagcaatctt
    33301 gttcagttca gccagagctt cgttgaacat cggtacagtt ttctcaaggt tgatgatgtg
    33361 aactttgtta cgcgcaccga agatgaacgg cttcattttc gggttccagt aacgggtctg
    33421 gtgaccgaag tgaacaccag ccttgagcat gtcgcgcatg gaaacagttg ccatgattaa
    33481 aacctctata taaaagttgg ggttatgcct ccacgtatcc catattaccg accccaaagg
    33541 gcaccccgga atatgtgccg atacgtgtgt gttgttacac aaagtgagat tgtcgcttcc
    33601 gtccagtctg tgtatacgaa atggatcgga agtccggcgc gctttatacc acaaatacgt
    33661 catggacacc aataattgtt ggcgctgtgt acagcatcag atgtcgaatt ttctattata
    33721 gaaaaccttc agtggcacgt ttggcgaaat tcagaatgat tctcaatttg cccgggtgtg
    33781 ataccattga cggcacttac atatatattg tcggtatcac cgacgctgat ggacagaatt
    33841 aatggctatc tcaatcaaga ccccagaaga tatcgaaaaa atgcgcgtcg ctggccgact
    33901 ggctgccgaa gtgctggaga tgatcgaacc gtatgttaaa ccgggcgtca gcaccggcga
    33961 gctggatcgc atctgtaatg attacattgt taatgaacaa cacgcggttt ctgcctgcct
    34021 cggctatcac ggctatccga aatccgtttg catctctatt aatgaagtgg tgtgccacgg
    34081 tatcccggac gatgctaagc tgctgaaaga tggcgatatc gttaacattg atgtcaccgt
    34141 aatcaaagat ggtttccacg gcgatacctc gaaaatgttt atcgtcggta agccgaccat
    34201 catgggcgaa cgtctgtgcc gcatcacgca agaaagcctg tacctggcgc tgcgcatggt
    34261 caaaccaggc attaacctgc gcgaaatcgg tgcggcgatt cagaagtttg tcgaagcaga
    34321 aggcttctcc gtcgttcgtg aatattgtgg acacggtatt ggtcgcggct tccatgaaga
    34381 accgcaggtg ctgcactatg actcccgtga aaccaacgtc gtgctgaaac cggggatgac
    34441 gttcaccatt gagccaatgg tcaacgcggg taaaaaagag atccgcacca tgaaagatgg
    34501 ctggacggta aaaaccaaag atcgcagctt gtctgcacaa tatgagcata ctattgtggt
    34561 gactgataac ggctgcgaaa ttttgacgct acgcaaggat gacaccatcc cggcgataat
    34621 ctcgcacgac gaataagatg aagccggcga atgccggctt ttttaatgcg ataatttaat
    34681 cttatgggtg gcgcacaatg aatacccttc cagaacagta cgcaaacacc gctctcccca
    34741 ccctgcccgg tcaaccgcaa aatccatgcg tctggccccg tgatgaatta accgtcggtg
    34801 ggataaaagc ccatatcgat actttccagc gttggctggg tgatgccttt gacaatggga
    34861 tctctgcaga acagttgatt gaggcgcgca ccgagtttat cgaccagctc ctgcaacgat
    34921 tatggattga agcgggattc agccagattg ccgacctggc attggtcgcc gtcggtggct
    34981 acggtcgtgg cgagctgcat ccactttctg acatcgattt actgatttta agccgtaaaa
    35041 agctcccgga cgatcaggcg caaaaagtgg gcgagctgtt aacgctgctc tgggatgtaa
    35101 agctggaagt cggtcatagt gtgcgcacgc ttgaagagtg catgctggaa gggttatcgg
    35161 atttaaccgt cgccaccaat ttaatcgaat cccgcttatt aattggcgat gttgcgctct
    35221 tcctcgaact gcaaaaacat atttttagcg aaggattctg gccttccgac aagttctacg
    35281 cggcgaaagt tgaagaacag aaccagcgcc atcagcgtta ccatggcacc agctacaacc
    35341 ttgaaccaga catcaaaagt agccctggcg gcttgcgcga tattcacact ctgcaatggg
    35401 tggcccgccg tcattttggc gcaacatcgc tggatgaaat ggtcgggttt ggcttcttaa
    35461 cctcagcgga gcgggcggaa ttaaacgaat gtctgcatat actgtggcgt attcgctttg
    35521 ccctgcatct ggtcgtcagc cgttacgata atcgtctgtt attcgatcgc caacttagcg
    35581 tcgctcagcg tctgaattac agtggtgaag gcaacgaacc ggtcgagcgg atgatgaagg
    35641 attacttccg cgttacacgc cgcgtcagtg aactcaacca gatgctgctg caactgttcg
    35701 atgaagccat cctcgccctt cccgccgacg aaaaaccacg tccaatcgac gatgagtttc
    35761 agctacgcgg tacgctaatc gacctgcgtg atgaaacact atttatgcgc cagccggaag
    35821 ccatcttgcg tatgttctac accatggtgc gcaacagtgc gatcaccggc atttactcca
    35881 ccacgctgcg ccagttacgc catgcccgtc gccatctgca acaaccgctg tgtaatattc
    35941 cggaagcacg aaaactgttt ttgagcattc tgcgtcaccc cggagcggtg cggcgcgggc
    36001 tattgccaat gcatcgccat agcgtgctcg gcgcgtatat gccgcaatgg tcgcatatcg
    36061 tcgggcagat gcagtttgat ctgttccacg cctacacggt ggatgaacat actatccgcg
    36121 tgatgctgaa actggagagt tttgccagtg aagaaacgcg ccagcgccat ccgttgtgtg
    36181 tggacgtctg gccgcgcctg ccgtcaactg agctgatttt catcgccgcg ctgtttcacg
    36241 atatcgccaa aggacgcggc ggcgaccact ccattctcgg tgctcaggat gtagtgcatt
    36301 ttgcagaact ccacgggctg aactcacgcg aaacacagct ggttgcctgg ctggttcgcc
    36361 agcacttgct gatgtcggtg acagcacaac gccgcgatat tcaggaccct gaagtcatca
    36421 agcagtttgc cgaagaagtt caaacggaaa atcgtctgcg ctatctggta tgcctgacag
    36481 tggctgacat ttgcgccacc aacgaaacgc tgtggaatag ctggaagcaa agtctgttgc
    36541 gcgaactcta ttttgccacc gaaaagcagc tacgacgcgg aatgcaaaac acgccggata
    36601 tgcgcgaacg ggttcgccat caccaactcc aggcactggc actactgcgc atggataaca
    36661 tcgacgaaga ggcgctgcac caaatttggt cacgctgtcg tgctaactat tttgtccgcc
    36721 atagcccaaa tcaactggcc tggcatgccc gccatttatt acagcatgat ttaagcaaac
    36781 cgctggtatt gcttagcccg caggctacgc gtggaggcac cgagattttt atctggagcc
    36841 cggaccgccc ctatctgttt gccgccgtct gtgccgaatt agaccgccgc aatttaagtg
    36901 ttcacgacgc acaaattttc accactcgcg acggtatggc gatggatacc tttatcgtgc
    36961 tggaacccga tggcagcccg ctgtccgcag atcgtcatga ggttattcgg tttggtctgg
    37021 agcaagtact gacgcaaagt agctggcagc caccgcagcc ccgtcgccaa cccgccaaat
    37081 tacgccattt tactgttgaa accgaagtaa cgtttttgcc gacccatacc gaccgcaaat
    37141 cgttccttga gcttatcgcg ctcgaccagc caggactgct ggcgagagtc gggaaaattt
    37201 ttgccgatct gggaatttcg cttcatggtg cccgaattac aaccattggc gagcgagtag
    37261 aagatttatt cataattgcc accgctgacc ggcgtgcact taataacgag ttgcagcagg
    37321 aagtgcatca gcggttgaca gaggccctca atccaaacga taaagggtga tgtgtttact
    37381 gatatgaaaa gagtttaaca atgcagcagt tacagaacat tattgaaacc gcttttgaac
    37441 gccgtgccga gatcacgcca gccaatgcag acaccgtaac ccgcgaagcg gtaaatcagg
    37501 tgatcgccct gctggattcc ggcgcactgc gtgtagcgga aaaaattgac ggtcagtggg
    37561 tgacgcatca gtggttgaaa aaagcggtgc tgctctcttt ccgtattaat gataatcagg
    37621 tgatcgaagg ggcagaaagc cgctacttcg ataaagtgcc gatgaaattc gccgactacg
    37681 acgaagcgcg ttttcagaaa gaaggcttcc gcgttgtgcc accagcggcg gtacgtcagg
    37741 gtgcatttat tgcccgtaac accgtgctga tgccgtctta cgtcaacatc ggcgcatatg
    37801 ttgatgaagg cacgatggtt gatacctggg cgaccgtcgg ttcttgtgcg cagattggta
    37861 aaaacgtcca cctttccggt ggcgtgggca tcggcggcgt gctggagccg ctgcaggcta
    37921 acccgaccat tattgaagat aactgcttca ttggcgcacg ctccgaagtg gttgaagggg
    37981 tgattgtcga agaaggttcc gtcatctcta tgggcgtata cattggtcag agcacccgta
    38041 tttacgaccg tgaaaccggc gaaatccact acggtcgcgt tccggcgggg tctgtggttg
    38101 tttcaggtaa tctgccgtcg aaagatggca aatacagcct ctactgtgcg gttatcgtta
    38161 agaaagttga cgcgaaaact cgcggcaaag tcggcattaa cgaactgctg cgtaccatcg
    38221 attaagagta catatacggg cagcatgatg ctgcccgtat gctttctggc tcagaataat
    38281 tcgtcctact cattcttctt cccccctttt ttaaatactt tttcatcttg tttcggactg
    38341 tcatgtaatt cgcaaaacga agataaatgt gttaatccat cccatggaat atcgccacaa
    38401 taaagcaggt aattttttga tttttacgcc atgatagaag tctggaagaa aaaggctttc
    38461 tgcgatcaca gtcgagccgg gcgggccagg ccaaatgcta aaataagcac aatttactga
    38521 ctcattttga ggtaacgcta tgtacgacaa tctgaaaagt ctgggtatta ccaatcctga
    38581 agaaattgat cgttacagcc tccggcagga agccaacaac gatattctga aaatctattt
    38641 ccagaaagac aaaggcgagt ttttcgccaa gagcgttaag tttaaatatc cgcgtcagcg
    38701 taaaacggtc gtcgctgatg gtgtgggtca gggttataaa gaagtccagg aaatcagccc
    38761 gaatctacgg tatatcattg atgagcttga tcaaatctgc cagcgtgacc gcagcgaagt
    38821 tgatcttaag cgtaagatcc tcgacgactt acgtcacctg gagtcagtcg taaccaataa
    38881 gatcagcgag attgaagccg atctggaaaa actaacgcgt aaataatgcc gaactgacgg
    38941 ttaactcgga cattcccctc tacccttcgg gctgagggga atgagccagc gccgcctcga
    39001 ccttaacgca taacctaccg ctcttcatcc agctgcaacg ccacatacag cagcaacctg
    39061 tcatcaaaat tacccaaatc aagcccggtc agttccgata tacgattaag ccgatactcc
    39121 agagtattac gatgaataaa caacgccttt gacgttgcca gcggttgcac attgtgacga
    39181 aaccacgccg ccagcgttcg tcgcagcaag ccgttattgt ccatcgcttt cagccgcgcc
    39241 agcggtcgcg ccagttcgtt ggcctgccag tcgccacgca aactgtcgag taacacaggt
    39301 aacatcagat cctgataaaa atagcagcga ctttctggca tccgctgttt acccaccacc
    39361 atcgtcgttt tcgccgtacg ataggatcgg gcaatactgc caggaccggt aaaatagttg
    39421 cccagtgaaa cgcgaaaacg cagctggccg tactctttca tgcgggtaat cagttgttca
    39481 actcgcttac gatgatcttc tgcatcccag cgcccaaaag agttcaacgc cggtttcaac
    39541 accaccattt cggttagcga gacaatcgcc accagattat tacgctcggg cgtagtcagc
    39601 gcgttttgca gttgttgtaa ctccgccatt gcgctgtcca caccaagctg accgctgtcg
    39661 acctcaacaa tagccaccac tcgcggttga ttgagatcga tccccagccg ttgcgcccat
    39721 tcagtaagtg cgggagtatt ctcctctgcc tgaatcaggt tcatcaccag ttcctcccgc
    39781 aaacggctat cctgagccaa caagtgcatc aaccgcgact gttccagcat catttcagcc
    39841 gtcatgcaga ccagttcgcc atatttacgc agattctctg gttcacctgt caggccaatt
    39901 acgccgacaa tttcaccttc cagccgtaac ggtagattaa tcccttgccg cacaccgtgc
    39961 agatgacgtg ccaccgcgtc atcgatatcg acgactcgtc cctgtgaaag taccagcaat
    40021 gcaccttcgt gcaattcacc aatacgctca cgatcgccgc tgccgataat tcgcccacgg
    40081 gcatccatta cgttgatatt ggtatcgatg atgcgcatgg tacgtgccac gatatcctgc
    40141 gccattttgg tatcaagatg ccagccagcc ataagtcctc cgttatgcac ggcttagcat
    40201 aaggaagtac gtaacgtacg gcattgtgca aatgcctaaa ggatgatgaa gatgtatgga
    40261 gttgtggtga agttcacaga ttgtaaggag aaccccttcc cgttttcagg aaggggttga
    40321 gggagattac tgcattaaca ggtagatgga gctgtcgccg cgctgaatgt tcagtgccag
    40381 cacagacggt ttgctgtcga gaactttacg cagttcagcg atgtttttca ctgcctgctg
    40441 gttcgcgcca ataatcacat cacctttctt caggccgatc tgcgcagccg gagtgcccgt
    40501 tttcacgttg ttcactacca cgccctgatc tttgcctttg ttgctcatct cagcaccttc
    40561 aatgccgttg aagatggagc tggaatcaac ctgattctgg ctgctctgct gcagttccag
    40621 attcacgttg acctgcttac cgtcgcgcag caagcccagg gtcagtttgc tgcccaccgg
    40681 catagtaccc acctgagcac gcagtgcggc aaagctgctg attggcttac cgttcagtga
    40741 ggtgatcaca tcacccgctt taatgcccgc ttttgcagcg gaggaattag gcagaacctg
    40801 gcttacgaaa gcaccgcgct gggcgtcaac tttcatcgct ttcgccagtt cggaattcag
    40861 ctcagtcccc ataataccca gctcaccgcg tttcacctgg ccgtattcca ccatctgcga
    40921 ggtcaggttt ttcaccatgt tgctcgggat agcaaaaccg ataccgatgt tgccgccgtc
    40981 cggtgcgagg atcgcggtgt tgataccgat cagttcgccg ttcaggttaa ccagcgcacc
    41041 accggagtta ccacggttga tcgctgcatc ggtctggatg aagttttcgt agttttcggc
    41101 attcaggccg ctacgcccca gcgcagagac aatcccggaa gttaccgtct ctcccaggcc
    41161 aaacgggtta ccaatcgcta cggtgtaatc acccacgcgc agtgcatcag aatccgccat
    41221 cttaattgcg gtcaggtttt tcgggttctg gatctgaatc agcgcgatat cagagcgcgg
    41281 atctttgcca accatcttcg cgtcgaactt acgaccatcg ctcagctgga ctttaatcac
    41341 cgtcgcgtta tcaacaacgt ggttgttggt gacgacatag cctttatcgg catcaatgat
    41401 gacgccggaa cccagcgcca tgaatttctg ttgctggccg ccgccattac cgccctggcc
    41461 cccctggcag aacggagagc tctggaacgg tgaaccttcc tggcagaacg gagaatcatc
    41521 accgaagaac tgctggaaat tacgcggcat acgcggcgta ttaacggttg tgctaccttc
    41581 tacgttaatg ctgaccactg aaggcatcac cttttcgagc atcggtgcaa ggcttggcat
    41641 ctgctgggct gtcgttgctg aagaagtctc agccgccgtt gcagagagcg gagataacgc
    41701 caaacctaaa ctcagagcca gtgcactcag tgctaatgtg gtttttttca tgtatttcag
    41761 tctcgattaa cagataacgc aaaattgctg tgttcttcag attcgtttta tagcgcgaag
    41821 ttccgaacta aagtttctgc aaaaggtaaa aatttattgt tcgtctttac aaaagcctgg
    41881 ttattgttct acggccatca gacgtcggta ttcatcccac gcatagaggt cggtcatacc
    41941 gctgatataa tcctgcaaca ggcggcaacg gtaataatat tcccatagcg gaaactcagg
    42001 agaatctgac ggtaatttac tgacagcctc gacataggcc agccgatggc gcgtcgagag
    42061 tttgtggaat aagcgcgatt caatagggaa acgtttcacc cgttcttttt ctaccagttc
    42121 agtaaagtct gataacgaca ggcttaataa aggacgataa atctctaata atccgctaat
    42181 gacccgatag ccctgcaatt caagctgctc gacatctgga tggctaaaca catgttttac
    42241 agcgacattt ttatatagct taagaagatc gctgcattcg ctggcatctt ccaataatgc
    42301 atgattaaac gttccggcga aaatcgcagg cagattatca ataaatcgtt gtgccgcgta
    42361 gggtaccagt ttatttaggg tgtttacccg taaatacata aaaaactgat cttccgtact
    42421 gcggcttaaa ctatttgagc gtgatttttc ccaggcattt tcaaccacca gcgaaaagag
    42481 cgaacctttc tcatgctggc cccacgcttc gtgcaaatga tgataaagct gctcaacggt
    42541 aaatattctt ttctctactg catcttcaag gtctgccaca caataggaga tatcgtcggc
    42601 agcttccata atccacgtta atggaaaacg actgtaaagc gccaaattaa gttctttacg
    42661 caaccgggca atataggctt cttcagaaag ataataaccc ggctttttca ttaaatagtg
    42721 atgtgtctca ggcgtttcgc cacgccacca cgccggacgg gtatatttta aaataccgcc
    42781 aacctgtgcc caggtgagat tcatccgcat caatgtatgc accagacgaa tgccttgtgc
    42841 attcccctca aaataacata agtcctgacg aatcttgcgc cgcagctcgt taagcggttc
    42901 ttccccgtcc cgtaaacgta gtgctgccac gctgcagcga tcgtcagtca gaggctggct
    42961 ttcggcatct tccgggtata aacgctggcg aaaccagtca tttatcgccg cttcgccaaa
    43021 atgaccaaac ggcggattgc cgatatcgtg catcaggcat gacatctcaa caatgctttc
    43081 aaaggggccg gtcagttcat ccaggccgta tgcttccagt aatttaagct ctttcagacg
    43141 gcttagaatt tctttggcga tgtagcgccc cacctgctgg acttccatcg agtgggtaag
    43201 acgcgtgcgc acggcggcat tgcgctccag tggaaaaacc tgggtctttt gttgcagacg
    43261 acgaattgcc ggagagttga tgatacgccc gcgatcgctc tcgaagatcc gcaggatctc
    43321 atgttcggtt ttaacgccct gcggtgaacg gtaacgacga tgccagttta ttttttttcg
    43381 gaaatcaatc tgtgccatgt cctctcccgc gtgagaaata cgcttccccg taagcgcatg
    43441 gtaaactatg ccttcaaatc gggcttatcg cgagtaaatc tatgaaaatc ggcatcattg
    43501 gtgcaatgga agaagaagtt acgctgctgc gtgacaaaat cgaaaaacgt caaactatca
    43561 gtctcggcgg ttgcgaaatc tataccggcc aactgaatgg aaccgaggtt gcgcttctga
    43621 aatcgggcat cggtaaagtc gctgcggcgc tgggtgccac tttgctgttg gaacactgca
    43681 agccagatgt gattattaac accggttctg ccggtggcct ggcaccaacg ttgaaagtgg
    43741 gcgatatcgt tgtctcggac gaagcacgtt atcacgacgc ggatgtcacg gcatttggtt
    43801 atgaatacgg tcagttacca ggctgtccgg caggctttaa agctgacgat aaactgatcg
    43861 ctgccgctga ggcctgcatt gccgaactga atcttaacgc tgtacgtggc ctgattgtta
    43921 gcggcgacgc tttcatcaac ggttctgttg gtctggcgaa aatccgccac aacttcccac
    43981 aggccattgc tgtagagatg gaagcgacgg caatcgccca tgtctgccac aatttcaacg
    44041 tcccgtttgt tgtcgtacgc gccatctccg acgtggccga tcaacagtct catcttagct
    44101 tcgatgagtt cctggctgtt gccgctaaac agtccagcct gatggttgag tcactggtgc
    44161 agaaacttgc acatggctaa gtcactgttc agggcgctgg tcgccctgtc ttttcttgcg
    44221 ccactgtggc tcaacgccgc gccgcgcgtc atcacgcttt ctcccgccaa cactgaactt
    44281 gcctttgctg ccgggatcac gccggttggg gtcagcagct attccgacta tcctccacaa
    44341 gcgcaaaaga ttgagcaggt ttccacctgg caggggatga atctggaacg cattgtcgcg
    44401 ctgaagcccg atctggtgat tgcctggcgt ggaggtaatg ccgagcggca ggttgaccag
    44461 ctggcttcgc tgggaataaa agtgatgtgg gtcgatgcga caagcattga acaaattgcc
    44521 aatgcgttac gtcaactggc cccctggagt ccgcaaccag acaaggccga acaagccgcg
    44581 caatccctgc tggatcagta cgcgcaattg aaagcgcaat atgctgataa acctaaaaaa
    44641 cgtgtttttc tgcaattcgg cattaatccg ccatttacca gtggaaaaga gtcgattcag
    44701 aaccaggtac tcgaagtttg tggcggagaa aacatcttta aagacagccg ggttccctgg
    44761 ccgcaagtta gccgcgaaca ggtgttagca cgctcgccac aggcgattgt cattacaggc
    44821 ggaccggacc aaattcctaa aatcaaacaa tactggggtg aacagctcaa aattcccgtt
    44881 attcctctca cgagtgactg gtttgaacgt gcaagcccac gtattatcct cgcagcacaa
    44941 cagctctgta atgcgctttc acaggtagat tagtgcctga ccttttcagg tgctcagtgg
    45001 ggattgaaca atgctcgtct attggctgga tatagtcggc acagcggtat ttgccatctc
    45061 cggcgttttg ttagccggaa aattgcgtat ggaccctttt ggtgttctgg tactgggcgt
    45121 ggtaaccgca gtaggcggcg gaacaattcg cgacatggcg ctggatcacg gcccggtatt
    45181 ttgggtgaaa gatcccaccg atctggtcgt tgcaatggtc accagcatgc tgaccatcgt
    45241 gctggtgcgc cagccaagac gcttaccaaa atggatgttg ccggtgctgg acgccgttgg
    45301 tctggcggtg tttgtcggca ttggcgtgaa taaagccttt aatgcggaag ccggtccgtt
    45361 aatcgcggtt tgtatgggcg tcattactgg cgttggcggc gggattattc gtgatgtact
    45421 ggcccgcgag atccccatga ttttacgtac agaaatctac gcaactgcct gtattatcgg
    45481 cggtattgtc cacgctacgg cttattacac attttccgta ccactggaaa cagccagtat
    45541 gatgggcatg gtcgtgacgc tattgattcg gctggcggct attcgttggc atcttaagct
    45601 accgacgttt gcgctggatg agaatgggcg ttgaagagag aagatgtgcc ggatagttta
    45661 tccggcacat gaacaacaga ttagatgcta aaggaagaac cgcaaccgca ggtgcttttc
    45721 gcgttcgggt tggtcacgat gaaacgagaa ccttccagtc cttcggtata gtcaacggaa
    45781 ccgccgacca gatattgcag gctcatcgga tcaaccacca ggccaacgcc ctgtttttcg
    45841 atggtcatat cgtcttcgtt cacctgatca tcaaaggtga aaccatactg gaagccgctg
    45901 caaccgccac cggtgatata cacgcgtaat ttcagattcg ggttatcttc gtcagcgatc
    45961 aggcttttaa ctttgttggc tgctgcgtcg gtaaactcca gcggcagtgc tacgtcatca
    46021 ctcatatttt gctccaaacg acatcggcaa ttgggcaaat tctaacccaa taataatcgc
    46081 cattatctaa taccctggta tttcgttcaa gtattctcgc tggctgatgc ggccttgctt
    46141 cgcgccagtt gctcagcttc ctgttttgcc agcgtgcgcg caagaatcgc cgagtatagc
    46201 ggtttcccgc cggtaaattg cgctaatagt gttgcgccaa gaccggtaat aatcattggc
    46261 aaaatgagct ggtagttatc ggtcatctcc agaaccagaa tgatccccgt taacggcgcg
    46321 cgaatagatg ccgccagtaa cgcccccatt ccggcaatag caaacgttcc cgcctcaagg
    46381 tgatattgcg gaaacagctc aacggcaacc attccgaaag cggttcccag cacagtaccc
    46441 agcgccagca tcggggcaaa aataccgccc ggcgcgccgg aagagaagca gagtaaggtg
    46501 gtaatgaccc gcgcgacgaa gataaacacc agcattccca tgctgaaatt ccccgcggta
    46561 gcgataggaa tcaggttaaa accgccgccc gacgttgctg gtgccacaaa ccccagcaat
    46621 ccacacagac cgccaatcgc accgcccatt agcacccatt tggtaatatt gccgccgtgc
    46681 acacggtgca gcaaatcctg catccccagc acccatttat taaaaatagg gccgaaaatg
    46741 ccaaaaataa taccgaggat cagataaagc cacagcgtat taagcggcgc atcagaaagt
    46801 ttaccgacgt caatcaacgc aacttcatga ttaaaaatcc ggtacataat ggtcgacata
    46861 atgacgccaa taaataccgc tttaatcgaa attaacgtat agcgaaactg cggacgcatc
    46921 tcttcgataa taaacaaaat acccgccagc ggcgcgttaa aggccgcagc cagccccgca
    46981 gcagcaccgg ttgccagcag cgtatggcga gcttcatcac ctttcaggcg gaaaatatca
    47041 agcaccatgc gaccgatatt gccgccgatc tgcacggttg gtccttcgcg ccctagcacc
    47101 atgcctccgc cgagtgtccc cagcccgcca aagaatttca ccggcaatac acgccaccag
    47161 cgaacaggac gttgatcttc cagcgcccct tcaatttccg ggatccccga tccccctgct
    47221 tcgggcgcgt atttgcgcac caaaaagtag ccgaacatcg ccagcaccgc cgaacagaga
    47281 aaagcgacag ttaacagtag cggataatta tcagcagtat gtaccagcgc ccccatacgt
    47341 tggttctgca accaggcgac acctttgtca aaagcaaccg cagccagccc gacaagcgtg
    47401 ccgacgactg ccgccataaa caaaatggct aacggggttt tatcgcgctc aagaagttgg
    47461 cgaatcagtt gtctgcgtcg caggcgcgcg gcctgcggtg tttctaaaga gggagtatca
    47521 gttttcatcc aatgatcact tattggtcat acaaataaga tgacggcatt ttactcgcac
    47581 gttccatgaa aatcctttgc aaagcgtaat gtttcaaatg cacaaaactt ttatactcta
    47641 agaaatcagg attgcaccag tacaagcagc ctgatgtttg acgagtattt aacttgttat
    47701 gaataacata gaatagcagc cattcacttt ttctacgaat caggaaccct ccatgagtaa
    47761 gtctgaaaat ctttacagcg cagcgcgcga gctgatccct ggcggtgtga actcccctgt
    47821 tcgcgccttt actggcgtgg gcggcactcc actgtttatc gaaaaagcgg acggcgctta
    47881 tctgtacgat gttgatggca aagcctatat cgattatgtc ggttcctggg ggccgatggt
    47941 gctgggccat aaccatccgg caatccgcaa tgccgtgatt gaagccgccg agcgtggttt
    48001 aagctttggt gcaccaaccg aaatggaagt gaaaatggcg caactggtga ccgaactggt
    48061 cccgaccatg gatatggtgc gcatggtgaa ctccggcact gaagcgacca tgagcgccat
    48121 ccgcctggcc cgtggtttta ccggtcgcga caaaatcatt aaatttgaag gttgttacca
    48181 cggtcacgct gactgcctgc tggtgaaagc cggttctggc gcactcacgt taggccagcc
    48241 aaactcgccg ggtgttccgg cagatttcgc caaacatacc ttaacctgta cttataacga
    48301 tctggcttct gtacgcgccg cgtttgagca atacccgcaa gagattgcct gtattatcgt
    48361 tgagccggtg gcaggcaata tgaactgtgt tccgccgctg ccagagttcc tgccaggtct
    48421 gcgcgcgctg tgcgacgaat ttggcgcatt gctgatcatc gatgaagtaa tgactggctt
    48481 ccgcgtggca ctggctggtg cacaggatta ttacggcgta gtgccagatt taacctgcct
    48541 cggaaaaatc atcggcggtg gaatgccggt aggcgcattc ggtggtcgtc gtgatgtaat
    48601 ggatgcgctg gccccgacgg gtccggtcta tcaggcgggt acgctttccg gtaacccaat
    48661 tgcgatggca gccggtttcg cctgtctgaa tgaagtcgcg cagccgggcg ttcacgaaac
    48721 gttggatgag ctgacatcac gtctggcaga aggtctgctg gaagcggcag aagaagccgg
    48781 aattccgctg gtcgttaacc acgttggcgg catgttcggt attttcttta ccgacgccga
    48841 gtccgtgacg tgctatcagg atgtgatggc ctgtgacgtg gaacgcttta agcgtttctt
    48901 ccatatgatg ctggatgaag gtgtttacct ggcaccgtca gcgtttgaag cgggctttat
    48961 gtccgtggcg cacagcatgg aagatatcaa taacaccatc gatgctgcac gtcgggtgtt
    49021 tgcgaagttg tgatgatttt gcctcgccca ccgggcgagg cattcaatta acggctctgc
    49081 tttctcaaca aatagataaa atatggcgcg ccgataaagg ttgacagcag ccccgccggg
    49141 atctggaatg gaaacagcac catccgccca caccagtcag cgaacaccag cagtaaacca
    49201 cccaccagcg ccgaaattac gatgtgcggc atcgtccgtc gaaagcccat catccgcgca
    49261 atatgcggtg ccattaaacc aacaaaactc aacggtccaa tagtcatcgt cgcggtcgcc
    49321 gtcaggcaag ccgctaacag cagcagcgca attcgcgtcg gcgttagcgc cattcctacg
    49381 gctcgggcgg tatcaccacc cagcggtaaa atggtcagcc agcggcggca cagcggggta
    49441 atcgccagca aaatcaccat cacaattccg gtgcgccaga cctgcgcatc ggtcgcgttg
    49501 taggtcgaac cggaaatcca ggtcagcact tgcgccattc gcgggtcacc acttgcctgc
    49561 aacatcatca aaagcatggt gaacgcggta cttaacgcca tccccgccag taacatgcgg
    49621 tgtggggaaa atccaccgcg tccggcggcg atcataatga tcaacagcgt caccgccgcc
    49681 ccgagactgc ctgcaggtaa caaccagcca aaggcattac ccggcaccag aaacagcatc
    49741 aacaccacgc caaacgctgc gccagagcta atccccagca cttccgggct tgccatcggg
    49801 tttccggtca gccgctgaat aatacagccc gccaccgcca gcatgacgcc cgcaaacagc
    49861 gccgccataa ttcgcggcca gcgccagggc attaaatcat cgagcagcgc cccgctcgcc
    49921 cacgtccagc cgtgcgcatc acgaccaaac gacagcgcca ccaccacagc aattatcagc
    49981 agcacaccgc cagcaagggc aaacgccagc acatgttggc gttcagccgc gacacgatcg
    50041 ttgaccttca tatccggcgc gctaatgctg cgtaaacgcg gcaacagcca cagtagcagc
    50101 ggcgcaccga tcaacgcagt gaccgaaccg gtggacactt ccatccacac gcgagtcagc
    50161 cagaggatga tttgatcgga aagccagaag atcagcgccc caatcagcga cgccagcatc
    50221 agtcgtggca gcagacgccg cgcccccagc atttttgcca gcagcggcgc gaacaacccg
    50281 ataaagccga taatccccac agcgttcacc agcagcgcac tgatgacaat cgccagcgac
    50341 agcgctgcca gacgcgcgag cgacaaggcc agcccgagat tgcgcgccac gccatcatca
    50401 agccccatca gggttaacgg gcgaagtagc aataacgtca acatcacgcc gcccagcaac
    50461 tgcggccata aacgctcaac gccgccccag tcggtttgcg tcagcgttcc ggtgctccac
    50521 aggaacatgc tttgcagctg gtcgtgatgg aagataacca gtagctgatt aattgccccg
    50581 caataaaggc tcaccaccag ccccgcgaga ataagcgtaa ccggcgaaag ccgtttcccc
    50641 cacgcgacgc caaagacgat taagccaacg acacaagcgc ccaccagtgc ggcaaactgg
    50701 ctcgccatag cacctgggat cgcccaaagt gtggtgacgg taattcccag ttgcgcgcct
    50761 gtagcaacgc caagtgtcgt cggttccgcc agcggattac gcagcacttg ctgaaacagc
    50821 acgcccacca gccccaggcc tgcgcccacc agcagcgaaa tcgccagacg cggcaacaag
    50881 ctgtagtgaa aaatcatctg ctcgatgacg tcaatatccg gcgaccaggc ggcctgcgcc
    50941 cactgactgc gcggcagcgc ctgtgagaag ttcatccagg ttagcgccgt agcgacaatc
    51001 actaacagcg ccagcaataa cgccgggaaa agcgcaattc gtttactcac gctttacctc
    51061 cgatggcgtt atccagaaca cgcacaaagt gcattgccga aagcgttgca ccataaaacc
    51121 agactgcggg tacgcgctga aagcgccctg tacggacaaa cggcatggcc tgccacagcg
    51181 gggttgccat tagcgcatcc atgtctttgc tgttgtcgtg atcaaaacag agcacatcaa
    51241 cgtctttata cgccgccaga cgatcgatac tgacggcggt actgccccag aagttggttt
    51301 ccccttgcca ggcatttggg atgccgtact catcgagaat ttcctggaac aagctgtttg
    51361 gaccgaagac cagcatatgg cgcggatcga taagcgtcgt cagcagtaac gggcgtgcac
    51421 cacgcttcac aaagcggggt ttcatgctgc ggataaagtc ttcgtattgc gctaaatgcg
    51481 tttccgctgc gctttgcagg ttaagtaaat ctgccatttc cgtcagcgat ttacgcgcca
    51541 tcgccaacgg ctgtttgccg tcactgaagt taaatccgcg acccggcgca atacgtgcca
    51601 gcatttctga tgaagggcca tatcctgccg accagaccat aaacgacggt ttcatttcgg
    51661 tcagcagttc aaggttaggt tctgtacgca aaccgacgtc aatcactgag tccggcaatg
    51721 gtggttcgct gacccacagg cgatagttga tggtatccgc cacgccgtaa ggcacgatgc
    51781 cgagcgccag cagtaattcc accggcaacc actccagcgc cacaatacga ttggggtcaa
    51841 tagccgccgc gtgggcggta ttcatctgcc ataacaacgg agaaagcgcc atcgccgtta
    51901 acagtcgacg gcgcgaaata agaggtaagc cgctcatcaa taaacaaaac tcacaggtgc
    51961 agcacccgcc ggatgcggca aaatacccat cgggatgcca taaatcattt cgagggtttc
    52021 gccgcgcata atttccgcag gcgttccctg agcaatcatt tcaccaccgc gcagggcgac
    52081 cagataatca cagtagcgtg ccgccatatt gatatcgtgc agcacggcaa tgaccgtcag
    52141 gccacgttcc tgacttaaac ggtgcaccag cgccagcaca tcaacctggt gggcgatatc
    52201 cagcgccgaa gtcggttcgt cgagcaacag acaacggcta tcctgcgcca ccagcatggc
    52261 gatccacgcc cgttgacgtt cgccgccaga gagactatcg accagccgat gcgccagcgg
    52321 ttttaagcca accagcgaga tagcttcctc gactttttcg cgatctgccg ccccaaagcg
    52381 ccccagcgcg ccatgccacg ggtaacgacc aatcgccacc agttcacgca cggtcaaccc
    52441 ttctgccgga ggaagctgct gcggcaaata agccactttg cgggcgaacg ctttgctgct
    52501 ccagctttcc agcggctggg tatcaagaag aatctcccct tccgtcggag gctgatgacg
    52561 gccaagcatt ttgagcagag tggatttacc agaaccgttg tgaccgatca gaccggtcac
    52621 tttcccggca ggaaaggtta acgacagcgg atgcaaaagc gtgcgcccgg gcacacgaaa
    52681 ggagatatta cgcagtgcaa aagtggtatc ggaatgattc gtgtattcct gcataacagc
    52741 caacttgtga aatgggcacg gtaatccgtg ccccaaaaga gaaattagaa acggaaggtt
    52801 gcggttgcaa cgacctgacg ttctgcgccc cagaagcagc cataagtctg gaagcaactg
    52861 gcaacatact cacgatcgag caaattgttg acgttcacag caaggctgga tcccgccata
    52921 ccaaagcgtg ccagatcata ttttacaaca gcatccatta cagctgcgct acccacttta
    52981 aaagtgtttg ccggatcacc atagctggaa ccaataaaac gaccacctgt tcccaatgtt
    53041 aaaccagaaa gcggcccttc attgaaggta taatcccccc agagagatgc catatgctcc
    53101 ggtacttgtt ctggagtttt acctttgaga ttggtgtctt tggtgtattc cgcatccgtg
    53161 taggtataag aagccgtcaa gttaatattg gcatttaacg ctgcttttgc ttcaagctcc
    53221 acaccacgag cgcgaatctc acctgctggg acttgcgcta acgggtttgt tgggtctgct
    53281 gtcaggttat ttgttttcgt caattgatag actgcgcccg taacaacgac cggcatatca
    53341 ttcggaacat attttacgcc agcttcatac tgttcacctt tcgatggctt ataggaaacg
    53401 cgcgggttgc tccacagatc gaaagcactc ggttcaaagg actggctata gctaaagtaa
    53461 ggtgaaatac cattatcgaa taagtaattt acaccaccgc gccaggtgaa ctgatgatca
    53521 ttacgctcaa tataaccttc agtcggcgta taagagtttt cacgaacagt ggtcgcttgc
    53581 ttagaccaat cgtagcgtcc ccccagagtg aatacccatt tattccattc cgcctgatcc
    53641 tgaacataaa tacctgtttg tttgctttca ttcatttggt atggctctgc gttaccaaat
    53701 gcaaagtatt caggatgata tttgttataa agatcgattg atggtgcaga tccaaagctg
    53761 gcattgatat cattgcgcat acgcatgaag tctacccctg tcagcaaagt atgctccact
    53821 tcacctgtag caaatttact ttcaagttgg gtatcaacgc taaagttttg cagacgctca
    53881 ttatccacca ctgtcccgcg gtttagagta tgaccatcgt tggcaatccc tgtgccataa
    53941 acacttttct gtgaggtttt catttcactg aaacgcagat tctgacgcac ggtgaaggtg
    54001 tcattaaaac catgttcaaa actatatccc accattttct ggttacggga gtatgtatta
    54061 ttcgatgcac cttcattgaa gtcagtcggt agacgtttac cattaggcaa tggctcaacg
    54121 gtcccctctt tcggcaacca accgtaataa cccgtttctg gttcattctg gaaataggac
    54181 aggaaagtaa aattggtttt gtcgtctgga cgccatgaga atgatggagc aatggtataa
    54241 cgctgggatt cagaattttt ctgctgttcg tttgttgaac gagcgaggcc cgtcaaacgg
    54301 taggagaact caccgttatc atccagtgca tcactgaaat cgaatccagt ctgaaacaga
    54361 ttatccgtcc ccattttaaa ctgaatttct ttcaggggtt cagtggtcgg acgcttactc
    54421 accatagaaa taatcccacc aggattactt tttccataga gaacagacgt cggcccacgc
    54481 atcagttcaa ccctctcgag catgtaagga tcaatcacag catcgttata aaagttgccc
    54541 tgaagtttta gtccatcaag gtaattattt tgattcagac caacagatga gaaaccacga
    54601 atgataacaa aatcatatgt attagaagca ccacgactgc taaccgtaac gccaggtgta
    54661 taacctaatg cttcttttac agactgaaat tgatgcatct gcatctcttc atttgtcaca
    54721 accgaaatag actgcggcgt tttttcaatt ggtgtatccg ttttagtcgc agtagcagag
    54781 tgcttcgccg caatagttgc agcaggcccc catgcgcttt cttgcggcgc aggtgcagcg
    54841 gtaacggtga tagtgtcttc tttcggttca accgctgcct gtgcataaac agacatgccg
    54901 ctaaccgctg tggctactac aactgcgatt ttacgcagtg agtgttttgg ctgagcagtt
    54961 ttggaacgcg ccattggtat atctctgatg taaagtgaat gataacgtaa acgagaatga
    55021 ttattataaa gataagataa tattcgaaac gctggcgggt tcgcaagcgg tacgcaatct
    55081 gagcgttcct gaagggttaa taaacaacca gatgaaaaga aagggttaat actttaggtg
    55141 ggatgttatt ttaccggatg gcaattcgcc atccggtatt tcacgcttag atgttaatta
    55201 ctaccaaaca tatccttgat ccaaccggct acaccgtcgc tgtctttctg ctcttgctga
    55261 gcaggttgct gttgcggctg ttgctgcggc tgagaagact gatcaaacgg attgcctgac
    55321 ggttgctgct gcatctcact ctgctggcac agcgattgcg gatcgctggt ccagaccggc
    55381 aagacacgca tgccaccgct gcaaacaaag ttgccgtcgt agtccacgcc catatctgca
    55441 atatcttctg gcggaacaag attcagcggc gttggcgtct ggttagccag ataacgctga
    55501 taaatcgaca ttgccccgct ggcaccatac agtttggtcg gctggttgtt atcacggcct
    55561 acccaggtga tggtcaccgt gctgccgtca atgcccgcaa accaggtatc tacgttattg
    55621 ttggtagtcc ctgttttccc tgccagatgc aggttcgggt atttcgcccc aagctgacga
    55681 cccgtaccgc gttgtaccac ctgctgcatg gtccatagtg tcagatacgc cgcctgcgcc
    55741 ggaacagcgc gttccgcctg cgggaagctc tgatacagca ccttgccatc ttccgcaatt
    55801 accgaacgca gtgcagaaag cggtgcacgg ttaccgccgc tggcgatggt ctggaacgcc
    55861 tgcgccactt cgattggcgt taagttcaac gcccccagca gcatcgccgg aaccggatgc
    55921 aactgatctt tcggtacgcc cagtttgatc caggtctccg taaccgcagg cagccccagc
    55981 gccatcccca aatttaccgt cggcacgttc atcgaacggg tcaacgcatc caccagcatc
    56041 actctgccgc tttcgctata acgacggtca tcattctgcg gtgaccagac ctggccattc
    56101 ggctgacgca gcgcgattgg cgcatccgca atccacgtat tcagacgata gattttcggc
    56161 tggcttaagg cagtcagata agtcgctggt tttgcaaggg aaccaatcga acgacgcgcc
    56221 tgcatcgcac ggttgtagcc cgcaaactgc ggctcagaac caccgaccat cgcacgaact
    56281 tcaccactaa agcggtcgac gaccacaatc gcagtttcaa gatcgctcaa cttacgctgt
    56341 ttcttcagtg ccggaatgcc ttccacggcg gctttttctg ccgcgtcctg ggccaccgag
    56401 tcaaaggtag tgaagatctt cacgccggag agatctttta ccttatcgcc taatttcgcc
    56461 tgcagctcct gacgcaccag ttgcataaag gctggctgtg gagagatcac cccaccgcgt
    56521 ggctgaacac ccagcggacg tgcactcaac atgtcataga gttcttgatc aataatctgt
    56581 tgctgttgca gcagacgcag caccagatta cgtcgctcca gcgccagttt tgggttacgc
    56641 cacgggttgt agatggacgc tcctttcacc atgccgacta acagcgcctg ctggtcgagg
    56701 cttagctctt ctaccgggcg accaaagtaa tacaagctcg ccagcgggaa gccgcggatt
    56761 tcgttgtcgc cgctctgacc gagatacacc tcgttcatat acagctcaag aatacggtct
    56821 ttgctgtaac gcgcgtccat gatcagcgcc atgtaagctt cgttcgcttt acgccagtaa
    56881 gaacgctcac tggagaggaa caggtttttc accagctgtt gcgtcagcgt actcgctccc
    56941 tgcaccgtgc gtccggcggt caggtttgcc agcaccgcac gtccgatgga gtagaggctg
    57001 atgccgtcat gctcgtaaaa atgacggtct tctgtcgcca gcaaagtatc caccagcaaa
    57061 tcagggaaac cactgcgcgg cacaaacaaa cgctgctcac cgtttggcga ggagatcatg
    57121 gtgatcagac gcggatcaag acggaagaaa ccgaactgac ggttgttctc catattgacg
    57181 atcgtcgcca gatgatcgcc atcaaaggtc agacgcgcac gcacctgccc ttctttactg
    57241 tccgggaaat caaacggacg gcggatcatc tcaatgctgt tggcctgcac ggtaaattcg
    57301 ccaggacggg tcattttcga cacctgacga tactgagtcg cctccagcag cttcaccatc
    57361 tcgttcttgc tgatggtcat atctggctca aggttgacca ttcggccata aactgccgca
    57421 ggcagttgcc agaccttgcc atcaatacgg ctacgaattt tttgatcgag ataaacgccg
    57481 taaatggcga tcagcacggc aaaaacgata gccagtttta acagcagcca tagccagccg
    57541 cgtttgccac gaggcttacg ccctttgcct ttgcccttac ctttgcgcgg catcggttct
    57601 tcatcctcat agtcatcata atcgtcgtaa tcatcgtcat cttcgtaacg acgacggctt
    57661 accttttgtt tgaccggacg cgtcggtttc cctttgcgtc caattggctc gcggtcattc
    57721 ccggccatgc tttttctccg caatattcag gcgcaaaagc ccgattttct gttcttctgt
    57781 tacaagacag aagaagatat ctctcaaaat ttgtgctcga tcggtcccct ctcccttgag
    57841 ggagagggtt agggtgaggg tgaacgtgcg cgctgatgcc ctcaccccgg ccctctccca
    57901 cagggagagg gagaaaacca accttacgaa tactttttcg tccgtcgcgt cggtgcagta
    57961 tttgccgggt cgtccggcca gacatgtttg ggataacgcc ctttcatctc tttttgcacc
    58021 tcacggtacg ctcctttcca gaaggcgctc aaatctcgtg tgatttgtaa tggcctttgg
    58081 gcaggtgaaa gcaactccag caccagcggc acgcgcccct gggcgatcgt cggattggtg
    58141 gcctcgccaa acatctcttg cattctcacc gccagcgcgg gcgggttatc ttcatgataa
    58201 cgaatggcga tccggcttcc cgtcggcaca gtgtaatgcg caggcaattc actatccaga
    58261 cgttgctgca ttccccaatc aagtaatccg cgtagtgcct gataaatgtc gagtgatttc
    58321 aggccgcgta gtgaatgtac gccagtcata tgtggcagca gccacgtttc cagcgtcgcc
    58381 aataaacttt catcatcaac cgctggccag tcatattccg gcaaccactt tgcggcgcat
    58441 aacaaacgca agcgtagctg ttccgcttcc gccgtccagt tgagcacgct taaatcttta
    58501 tcgcggatgc cattaagcat cgcctgatgc aactcgtctt ccgagggttt cgccagcggc
    58561 tgcactttca ccgtcagcag accgatttgc agccgacgcc aggctttcag cgtaccttgc
    58621 gcgtcatccc actccacagt gtcagactgc tgtaccagct gcgggcagcg ttgtactaac
    58681 tcatcaatat cgaccggcag cgccagtaaa atccgcgcat ccggcgaggc gctgccctgc
    58741 aataataacg gtgcgatcaa ccattcgtgg cggcttagcg cgtcgttggc atcgagcatc
    58801 gctcccatgc cgtttgccag ttgatagcgt ccatcttgcc cacggcgacg agcaatgcga
    58861 tcggcaaacg ccccggcaag tagcggcgcg ataagcgaac tgtctgcctc accgccacgt
    58921 acgtttaagc gttttaacag ttgctgacta cgttgctgcc aggctggttg attgcgcgaa
    58981 aacgccacgc ccaggtcact attgcccatc cgtggcggct cttcgagaat ggcggcaatt
    59041 tttgccgcgg tagcagcttc gtcgtcgttc ttcgcgctaa ccagcattgc cgctaaacgc
    59101 ggatcgttac ccagcgctgc cattttttgc ccttgcgcac taagccgttc accctccagt
    59161 gcccccagca tttgtaacag acgtttcgcg gccagtagat tcactactgg cggttgatcc
    59221 agccagctca tctgcgccgg atcgctgcat ccccattgca gtaattccat cagcaaaccg
    59281 gaaagatcgc tttgtaagat ctccggttca ctttgcgccg cggcgcgttc tgcttgttct
    59341 ttggcgatta aatgcaggca gatacccggc tccagacgcc cggcgcgccc ggcacgctgc
    59401 gtcatggatg cctggctaac gcgttgagta atcagtcgcg taagccccgt gcgcggatca
    59461 aaacgcgcca cacgctcctg ggcacaatcc accaccagac gaataccttc aatggttaaa
    59521 ctggtttcag caatattggt cgccagcacc actttgcgca tcccttgcgg tgccgggagg
    59581 atcgcttttc gctgatcgtt cagcgacaac gcgccataca gcgggcagag caatacatca
    59641 ctgccgatgc gcgaagccag ttgttcctgc acacgctgaa tttctccgac gccaggtaaa
    59701 aataacagta atgatccgct ttcctgacgc agcatttcag cggtggctac cgcaacggca
    59761 tcgtcaaaac gctgatgcgc gggcagcggt aaataacggc gttcaaccgg aaacgagcgc
    59821 ccttctgaga tgacgacagg cgcttctggc agcatttgct gcaagcggtc gttgtccagg
    59881 gtagccgaca taatcagcag tttaaggtca tcacgcagac cttgttgcac atcgagtaac
    59941 agcgccaacg ccaaatccgc ctgcaagctg cgctcatgaa attcatcaag gatcaccagt
    60001 ccaacaccgc tcagttccgg gtcacgctgg atcatgcgcg tcagcacgcc ttcggtaacc
    60061 acttccaggc gggtattcgg cccgacgcag ttttgcgcac gcatccggta gccaacggta
    60121 tcgcctggct tttcgttaag cagctccgcc agccgttgcg cgacgttacg cgccgccaga
    60181 cgacgcggct ccagcaggat aattttcccg ttaatgccgg gatgcgccag cagttgcagc
    60241 ggcagccagg ttgatttccc ggccccggtc ggcgcactta ataatacctg cggcgcacaa
    60301 tcgagagcgg taagtaattc agataagacg gcagcaacgg gcaacgacga cacaaaacgc
    60361 tccagagggt taacattctt cgcgccacat tgtagcatcg cggtaattca taaccgagtg
    60421 cctcacatgt ctgaaccgca acgtctgttc tttgctatcg acttacctgc agaaatccgc
    60481 gaacagatta tccattggcg cgccacacac ttcccacctg aggcgggacg tccggtcgcc
    60541 gccgataatt tgcatctgac tctggcattt ttaggcgaag tgagcgccga gaaagagaag
    60601 gcgctttctc ttttagccgg acggattcgt caacctggtt tcacactcac gcttgatgac
    60661 gccggacaat ggctgcgttc gcgtgtggtg tggttaggga tgcgtcagcc tccacgcggc
    60721 ttaatccagc tggcgaatat gctccgttca caggctgccc gcagcggttg ttttcaaagc
    60781 aatcgtccgt ttcatccaca tattacctta ttgcgcgacg ccagcgaggc ggtgacaatc
    60841 ccgccgccag gttttaactg gtcttatgcg gtgacggagt tcacccttta cgcctcctcg
    60901 tttgcccgtg gacgcacacg ctacacgccg ctaaaacgct gggcgctaac gcaataacaa
    60961 ggattgtcgc aatggaattc tctccccctc tacagcgcgc gacgctaatt cagcgttaca
    61021 aacgtttttt agccgatgtg atcacacccg atggtcgcga attaacgcta cactgcccga
    61081 atacgggtgc aatgaccggt tgtgcaacgc ctggcgatac cgtctggtat tcgacttcag
    61141 acaacaccaa acggaaatac ccacacacct gggaattaac tcaaagccag agcggcgcat
    61201 ttatttgcgt caacacgctt tgggctaaca ggttgacgaa agaggctatc cttaatgaat
    61261 caatttcaga actgtcaggc tatagctcgc tgaaaagcga agtaaaatac ggcgcagaac
    61321 gcagccgtat tgactttatg ttgcaggcgg attcgcgtcc agactgctat attgaagtga
    61381 aatcggttac gttagcggag aacgaacagg gatattttcc cgatgcggtc actgaacgag
    61441 gtcagaaaca ccttcgggag ttgatgagcg tagcggctga aggccagcgt gcggttatct
    61501 ttttcgccgt gctgcattca gccattacac ggttttcacc cgcgcgccac atcgatgaga
    61561 aatacgcgca actattgtca gaagctcaac agaggggggt agaaattctg gcttacaaag
    61621 cggaaatttc tgctgaaggc atggctctta aaaaatcact gccggttaca ttgtagtaaa
    61681 gtaagtaact ggttaattta cattctggtc gcgtgcgcaa atacgctttt cctcacacag
    61741 ttgtcaagtg ttacgtttag ataattgcta tccggaaaag catctgctat ttatagcggc
    61801 ctcatttttc ccccgaacat ggggatcgat agtgcgtgtt aaggagaagc aacatgcaag
    61861 aagggcaaaa ccgtaaaaca tcgtccctga gtattctcgc catcgctggg gtggaaccat
    61921 atcaggagaa gccgggcgaa gagtatatga atgaagccca gctggcgcac ttccgtcgta
    61981 ttctggaagc atggcgtaat caactcaggg atgaagtcga tcgcaccgtt acacatatgc
    62041 aggatgaagc agccaacttc ccggacccgg tagaccgtgc agcccaggaa gaagagttca
    62101 gcctcgaact gcgtaaccgc gatcgcgagc gtaagctgat caaaaagatc gagaagacgc
    62161 tgaaaaaagt ggaagacgaa gatttcggct actgcgaatc ctgcggtgtt gaaattggta
    62221 ttcgccgtct ggaagcgcgc ccgacagccg atctgtgcat cgactgcaaa acgctggctg
    62281 aaattcgcga aaaacagatg gctggctaat tacagccgtt ccatcacgtt taccacaggc
    62341 gggaaatgct cccgccttat tttttgttca aagagatgac agacacacag tatattggcc
    62401 gcttcgcccc ctctccttcc ggcgagcttc attttggctc tctgatcgcc gcgctcggca
    62461 gctatttgca ggctcgcgcc cggcaaggtc gctggctggt acgcatagaa gatatcgacc
    62521 cgcctcgtga agttcccggt gccgcagaaa ctatcctgcg ccagctggaa cattacggtc
    62581 tgcactggga cggcgatgtt ctctggcaat cgcaacgtca cgacgcctat cgtgaagcac
    62641 tcgcctggtt acatgaacaa ggactaagtt attactgcac ctgtacgcgt gcgcgtattc
    62701 aaagcattgg cggtatttac gacggtcatt gccgggtgtt gcatcatgga ccagacaacg
    62761 ccgcagtgcg tatccgccag cagcatccgg tcacgcaatt tactgaccag ctgcgcggca
    62821 ttattcacgc cgacgaaaaa ctggcacggg aagattttat cattcatcgc cgtgatgggt
    62881 tgttcgccta caacctggct gttgtggttg atgatcattt ccagggcgtt acagaaatag
    62941 tgcgtggggc tgatctgatt gaaccaacag taaggcaaat ctcgctgtac cagctttttg
    63001 gctggaaagt gccagattac attcatctgc cgctggcgct taatccacaa ggcgctaaac
    63061 tttccaagca gaatcatgcg cctgcgttgc cgaaaggcga tccacgcccg gtactaatcg
    63121 cggcacttca atttctgggg cagcaggcag aagcacactg gcaggatttc agcgtcgagc
    63181 aaatccttca gtcagccgtc aaaaactggc ggcttaccgc cgtgcccgag tcggcaattg
    63241 taaattcaac attctcaaat gcgtcatgct gagctatgat tagccgctat ttttttgtcc
    63301 tgaatgatgt ttgacactac cgaggtgtac tatttttacc cgagtcgcta atttttgccg
    63361 caaggtgcta agccgcgagg aaagcgaggc tgaacaggca gtcgcccgtc cacaggtgac
    63421 ggtgatcccg cgtgagcagc atgctatttc ccgcaaagat atcagtgaaa atgccctgaa
    63481 ggtaatgtac aggctcaaca aagcgggata cgaagcctgg ctggttggcg gcggcgtgcg
    63541 cgacctgtta cttggcaaaa agccgaaaga ttttgacgta accactaacg ccacgcctga
    63601 gcaggtgcgc aaactgttcc gtaactgccg cctggtgggt cgccgtttcc gtctggctca
    63661 tgtaatgttt ggcccggaga ttatcgaagt tgcgaccttc cgtggacacc acgaaggtaa
    63721 cgtcagcgac cgcacgacct cccaacgcgg gcaaaacggc atgttgctgc gcgacaacat
    63781 tttcggctcc atcgaagaag acgcccagcg ccgcgatttc actatcaaca gcctctatta
    63841 cagcgtagcg gattttaccg tccgtgatta cgttggcggt atgaaggatc tgaaggacgg
    63901 cgttatccgt ctgattggta acccggaaac gcgctaccgt gaagatccgg tacgtatgct
    63961 gcgcgcggta cgttttgccg ccaaattggg tatgcgcatc agcccggaaa ccgcagaacc
    64021 gatccctcgc ctcgctaccc tgttgaacga tatcccaccg gcacgcctgt ttgaagaatc
    64081 gcttaaactg ctacaagcgg gctacggtta cgatacctat aagctgttgt gtgaatatca
    64141 tctgttccag ccgctgttcc cgaccattac ccgctacttc acggaaaatg gcgacagccc
    64201 gatggagcgg atcattgaac aggtgctgaa gaataccgat acgcgtatcc ataacgatat
    64261 gcgcgtgaac ccggcgttcc tgtttgccgc catgttctgg tacccactgc tggagacggc
    64321 acagaagatt gcccaggaaa gcggcctgac ctatcacgac gctttcgcgc tggcgatgaa
    64381 cgacgtgctg gacgaagcct gccgttcact ggcaatcccg aaacgtctga cgacgttaac
    64441 ccgcgatatc tggcagttgc agttgcgtat gtcccgtcgt cagggtaaac gcgcatggaa
    64501 actgctggag catcctaagt tccgtgcggc ttatgacctg ttggccttgc gagctgaagt
    64561 tgaacgtaac gctgaactgc agcgtctggt gaaatggtgg ggtgagttcc aggtttccgc
    64621 gccaccagat caaaaaggga tgcttaacga gttggatgaa gagccgtcac cgcgtcgccg
    64681 tactcgtcgt ccacgcaaac gcgcaccgcg tcgtgagggt accgcatgac agtggcgtat
    64741 attgccatag gcagcaatct ggcctctccg ctggagcagg tcaatgctgc cctgaaagca
    64801 ttaggcgata tccctgaaag ccacattctt gccgtttctt cgttttaccg caccccaccg
    64861 ctggggccgc aagatcaacc cgattacctc aacgccgccg tggcgcttga aacttcgctc
    64921 gccccggaag agttactcaa ccatacacag cgcatagaac tgcaacaggg tcgtgttcgt
    64981 aaagctgaac gctggggacc acgcacgctg gatctcgata tcatgctgtt tggtaatgaa
    65041 gtgataaata ccgaacgcct gaccgttccg cactacgata tgaagaatcg tggatttatg
    65101 ctgtggccgc tgtttgagat tgcaccggag ctggcgtttc ctgatgggga gacgttgcgt
    65161 gaagtcttac atacaagagc atttgacaaa ttaagcaaat ggtaatttat ttatattacg
    65221 aatataaata ttttctgaaa atcatacatc ctctctttcc ttaataaaaa ttttagttta
    65281 aacatttttg cagaaacata atataaattc ccgcagataa atatcaatcc catcgccata
    65341 ccctaaccaa ttgcacttgt gaatttattt ttgcttaatt ttgaattaag aaaatttaat
    65401 ttgttcaaaa gcccttaaag tgcacaatag gcgtcctaat gaacaatggt tgtaaatttt
    65461 ggtaacaaat atcattgctc attaatatct agcatctgct tataacattt gtgtcatgaa
    65521 tagtcattct tcaaaccagt agtctgacta atggaatttt attcaaggat ttttatgtct
    65581 aaaaaattag gttttgccct gtctggtctt atgctggcaa tggttgcagg tactgcttct
    65641 gctgatatgg atggtggtca gttaaatatc agtggtctgg ttgttgataa cacctgtgaa
    65701 acccgcgttg acggcggtaa taaagatggt ctgatcctgc tgcaaactgc aactgttggt
    65761 gaaatcgatg ctggcgtgct gaatgacacc gttggcgcta aagccaaacc tttcagcatc
    65821 accgttgact gcagcaaagc aaatccgaat ccaggtagta cagctaaaat gaccttcggt
    65881 tctgttttct ttggtaatag caaaggaacg ctgaacaacg acatgtctat taacaacccg
    65941 tctgacggcg tgaatatcgc tctgcacaat attgatggtt ccactatcaa acaggttcaa
    66001 attaacaacc ctggcgatgt gtatactaaa gccctggatg caacgacaaa atctgctgtt
    66061 tatgatttta aagcgtctta cgttcgtgcc gtcgcagacc aaacagcaac tgctggttat
    66121 gtaaaaacta acactgcata caccattact tatcagtaag tattcaccaa ttaaattgat
    66181 tgaatgaata tacagggaat agtgttaata atttctattt ttatattatt ccctgtttta
    66241 attaactctg tcagggatgg tttatgtttt ttaacaccaa acatacaaca gctttatgct
    66301 tcgtaacctg tatggctttt agttcatcgt ctattgcgga cattgtcatt tcgggtactc
    66361 gcgtaatata taaaagcgat caaaaaagtg tcaacgtacg tctggaaaat aaagggaata
    66421 acccgttgct tgtccagagt tggttagata ctggcgatga caacgctgag cctggcagta
    66481 ttacagtccc ttttactgcc acgccgccag tatcgcgtat tgatgccaaa cgtgggcaaa
    66541 caatcaaatt aatgtacaca gccagcacct cactgcctaa agacagagag agcgtgttct
    66601 ggtttaacgt actggaagtt ccaccaaaac cagatgcaga aaaggtcgcg aatcaaagtc
    66661 tgctgcaact ggcatttcgc acacgtataa aacttttcta tcgcccggat ggattgaagg
    66721 gaaatccctc tgaagccccg ttagccctga agtggttctg gtcaggttca gaaggtaagg
    66781 cgtcattgcg agtgaccaat ccaacccctt actacgtctc ttttagcagt ggtgatttag
    66841 aagctagcgg taaacgctat ccgattgatg tgaaaatgat tgcaccattt agtgatgagg
    66901 tcatgaaagt cactggcctt aatggcaaag caagttctgc aaaagtacat ttttacgcca
    66961 ttaatgattt tggtggcgcc attgaaggta atgccagtct gtaatcaggc aaggatataa
    67021 ttccgcagga agcataacgt gactataaaa ccaactaaaa attatcataa tcatctgacc
    67081 cgtatcgcta cgttttgcgc gctgctgtat tgcaatagtg ctttctgtgc tgaacttgtt
    67141 gaatatgacc ataccttcct gatggggcag aatgcctcaa atattgatct cagccggtat
    67201 agtgaaggta accctgctat accgggtatg tacgacgtca gtgtttatgt caacgatcaa
    67261 cctattatta accaaagcat tacatttatt gaaattgaag gaaaaaaaaa cgcacaagcc
    67321 tgtatcacac ttaagaattt attgcagttt catataaatt ctcccgatat aaataacgaa
    67381 aaagccgttc tgcttgccag ggatgaaaca ctcggcaatt gccttaattt gacggaaatt
    67441 atccctcagg cttctgttcg ttatgacgtt aacgagcaac gtctggatat agacgttcct
    67501 caggcatggg taatgaaaaa ttaccaaaac tatgttgatc catcgttatg ggaaaatggc
    67561 attaatgcgg ccatgttgtc atacaacctc aacggatatc atagtgaaac tcctggtcga
    67621 agaaatgaca gcatttatgc tgcttttaac ggtgggatga atttaggtgc atggcgactg
    67681 cgtgcctcag gcaactacaa ctggatgacc gattctggca gtaattatga ttttaagaat
    67741 cggtatatac agcgtgatat cgcctcgctg cgttctcaac tcattcttgg tgagtcttat
    67801 acaacgggcg aaacctttga ttctgtcagt atccgcggca ttcgtttata cagcgacagc
    67861 cgcatgttgc ctccgacatt agcgagtttt gcgcctatca ttcatggcgt tgctaatacc
    67921 aacgccaaag tcactattac gcaaggtggc tataagattt atgaaacgac tgtgccgcca
    67981 ggcgctttcg tcattgatga tctgagtccg tcaggttacg gcagcgatct tattgttact
    68041 gtcgaagaat ccgatggctc aaagcggaca ttctcgcaac ctttttcatc ggtcgttcaa
    68101 atgttacgcc ctggcgttgg acgttgggat attagcggcg gccaggtctt aaaagatgat
    68161 attcaggatg agcctaattt atttcaggcc agctactact acggtctgaa taactatcta
    68221 acgggttata ctggtattca gattaccgat aataactata ccgctggttt gttaggtctt
    68281 ggtctgaata cttcagtcgg tgcattttct ttcgatgtga ctcactccaa tgttcgtata
    68341 ccggatgata aaacatatca gggacaaagt tatcgtgttt cctggaacaa gttattcgaa
    68401 gaaacaagta cttcactgaa tatcgcggcc tatcgctatt cgacacagaa ttaccttggt
    68461 cttaatgatg cactaactct aattgatgaa gtgaaacatc ccgaacaaga tcttgaaccg
    68521 aaatccatgc gtaattactc gcgcatgaaa aatcaggtta cggtcagtat taaccaaccg
    68581 ctaaaatttg agaaaaaaga ttacggttca ttttatcttt ccggaagttg gtccgattac
    68641 tgggcttccg gacaaaatcg tagcaattac tctattggct acagtaacag tgcatcctgg
    68701 ggcagctaca gtgtcagtgc ccagcgttca tggaatgaag acggcgacac tgacgatagc
    68761 gtttatctca gtttcaccat tcctattgaa aaattacttg gcactgaaca acggacttca
    68821 ggtttccaga gtattgatac tcaaatgagc agtgacttta agggtaataa ccaactcaac
    68881 gtcagtagca gtggctatag tgataacgct cgcctcagtt atagcgtaaa tactggctat
    68941 acgatgaata aagccagcaa agatttgagt tatgttgggg gttatgccag ctatgaatca
    69001 ccgtggggaa ccctggcagg ttcagtttct gcaaatagcg ataacagtcg tcaagtgtct
    69061 ctcagcaccg acggtggttt tgtattgcat agcggcggcc tgaccttcag taatgacagt
    69121 tttagcgact ccgatacact ggcggtagtt caggctccag gtgctcaagg agcgcgaata
    69181 aattatggca acagtactat cgatcgatgg ggttatggtg tcaccagcgc tctttctcct
    69241 tatcatgaaa accgtatcgc gctggatatc aacgatcttg agaacgatgt tgaattaaaa
    69301 agtaccagtg cagtagctgt accgcgtcag ggttcagtcg tctttgctga ttttgaaacc
    69361 gtgcaagggc aatcagccat tatgaacatc acacgaagtg atggtaaaaa tattccattt
    69421 gctgcagata tttatgatga gcaaggcaat gtcattggta atgttggaca gggtggacaa
    69481 gcatttgttc gtggcattga gcagcaggga aatatcagca ttaaatggct cgaagaaagt
    69541 aaacccgtaa gttgtcttgc gcattatcaa caaagttcag aagcagaaaa aatagcgcaa
    69601 tctattattc tgaatggaat tcggtgtcag attgagtaac tacaagggac gtcaaatgat
    69661 aaaaataacg ccacataaaa tagcgatact gatgggaata ttattatcac catcagtatt
    69721 tgcaacggat attaatgtag agtttacagc cactgtcaaa gcgacaacct gtaacatcac
    69781 acttactggt aataacgtca cgaatgatgg caataataac tacacattga gaatccctaa
    69841 gatgggtctg gataagatcg cgaataaaac gacagaatct caggctgatt ttaaactggt
    69901 tgctagtggg tgcagcagta gcatcagttg gattgatacc actctgaccg gaaatgcatc
    69961 atcaagctca cctaagctta ttataccgca gtctggtgat tcatcttcga cgacaagtaa
    70021 tatcggtatg ggtttcaaaa aacggactac tgatgatgcc actttcctta aacctaacag
    70081 tgcggaaaag atacgctgga gcacagacga gatgcagccc gataagggtc ttgaaatgac
    70141 cgttgcgcta cgtgaaacag atgcagggca aggcgtaccg gggaattttc gtgcactggc
    70201 aacgtttaat ttcatctatc aataagaagg ataattaaga tgacatttaa aaatttacgt
    70261 tatggattat ccagcagtgt cgttttggct gcctcattgt tcagcgtacc ctcttatgcg
    70321 gcaacagaca gcattgggct gaccgtgatt actaccgtag aaataggtac ttgtaccgct
    70381 acattagtaa atgactctga tcaggacatt tctgttgttg actttggcga tgtatatatt
    70441 tctgaaatca atgccaagac caaagttaaa acattcaaac tcaaattcaa agactgtgcg
    70501 ggtatcccca ataaaaaagc acaaataaaa ttaaccaagc gagccacatg cgaaggtact
    70561 gctaatgacg gtgcggggtt cgcaaatgga tccacagccg cagataaagc aagtgccgtc
    70621 gccgttgaag tctggagcac cgaaactccg gcaacaggta gcgcaacaca atttagttgt
    70681 gtgacaccag catctcaaga ggtcactatc tccaatgcag ccaacgcggt tgtttattat
    70741 ccgatgagtg ctcgactggt cgtcgaaaaa aataaaaccg taagcaatgt cactgcgggt
    70801 aaattttctg cacctgccac atttacagta acctataact aacagactga cagatccagg
    70861 aactatatat gcatcccact caacgtaagc tgatgaagag aataattctg tttctgtcac
    70921 tacagtttcc cattacctat ccagccatcg ctgggcagga tattgacctt gttgccaatg
    70981 taaaaaacag cacttgcaaa agcggaatca gtaaccaggg taatattgat cttggcgtcg
    71041 ttggggtggg atatttttca gataatgtta ctcctgaaag ctatcaacca ggtggaaaag
    71101 agttcactgt cacagtatca gactgtgcat tacagggaac tggcgatgtg cttaatcagt
    71161 tacatattga ttttagagcc cttagcggtg tcatggctgc aggttcaagg caaatatttg
    71221 ccaatgaagt cgcaaccggg gctaaaaatg tcggtgtcgt tctcttttcc attcaggacc
    71281 cgaccaatat attcaatgtt attagttcag caggaaattc tcgttccgtg tatccagtaa
    71341 tgacttctgc cctgcataat tcttcatgga aattttatgc cagaatgcaa aaaatcgatc
    71401 ccgcgctgga tgtcatatct ggccaggtaa tgagtcatat attagttgat gtgtactacg
    71461 aataatagat caaaacaaat aactgcgact gttacagcag tagatggtgg agtgataatg
    71521 aagaaattct tcagacattt tcttttttta attctgtgct tgagttgtta taccgcgagt
    71581 gctggcactg atgataacgt aggatatatt gttgggaata gttacggcgt tggtccgtca
    71641 gatcaaaaat ggagagaaac ggggccgaat ggcgatgcta ctgtcatatt cagatatgca
    71701 acaagtacaa ataatttagt tttctacaaa ccgacgcagc ttggacctac aggtgtaaaa
    71761 ttacagtgga gtcagttaga taccgcttct ggtggtggtt ttctttattg caacagaagt
    71821 gatagcacaa gtggtagcgc aatgcgtatt gaaaatgcaa tggttgactc aggtaaaatg
    71881 tatggctccc ataaattatt taatacatca gttcctggtt tgtattacac attattaatt
    71941 tcaaacatgt ggtcagctta cggtaccgta actaacgtta gttcacctgg gatatacatt
    72001 ggtgactctg cagaacaata tttttcgtgg tataatccaa gcgaagacgt gttatattgg
    72061 agttgcaata atgcgaatag cacccgtaaa tactgggctg taggtggtat ttatcagacc
    72121 cttacaattg aattctatac agatacaaac tttgatccaa cagtaactca acagatcaaa
    72181 ttatccagct catcaaatta tctgtattca tttaaagcct atggcgcagg ccaaggtata
    72241 aatgagcata gttattttat caaaatcgat ttcgatctgc tcaatgtcaa gttgactaat
//
LOCUS       DAFNBJ010000004        69504 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000004 DAFNBJ010000000
VERSION     DAFNBJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.004"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(54..962)
                     /gene="melR"
                     /locus_tag="L2X49_000263"
     CDS             complement(54..962)
                     /gene="melR"
                     /locus_tag="L2X49_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBN4795415.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1245..2600
                     /gene="melA"
                     /locus_tag="L2X49_000264"
     CDS             1245..2600
                     /gene="melA"
                     /locus_tag="L2X49_000264"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBN4795416.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2703..4124
                     /gene="melB"
                     /locus_tag="L2X49_000265"
                     /pseudo
     CDS             2703..4124
                     /gene="melB"
                     /locus_tag="L2X49_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
     gene            complement(4263..4892)
                     /locus_tag="L2X49_000266"
     CDS             complement(4263..4892)
                     /locus_tag="L2X49_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBN4795417.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(5015..6661)
                     /gene="fumB"
                     /locus_tag="L2X49_000267"
     CDS             complement(5015..6661)
                     /gene="fumB"
                     /locus_tag="L2X49_000267"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBN4795418.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(6739..8079)
                     /gene="dcuB"
                     /locus_tag="L2X49_000268"
     CDS             complement(6739..8079)
                     /gene="dcuB"
                     /locus_tag="L2X49_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBN4795419.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(8650..9369)
                     /gene="dcuR"
                     /locus_tag="L2X49_000269"
     CDS             complement(8650..9369)
                     /gene="dcuR"
                     /locus_tag="L2X49_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBN4795420.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(9366..10997)
                     /gene="dcuS"
                     /locus_tag="L2X49_000270"
     CDS             complement(9366..10997)
                     /gene="dcuS"
                     /locus_tag="L2X49_000270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBN4795421.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            11178..11408
                     /locus_tag="L2X49_000271"
     CDS             11178..11408
                     /locus_tag="L2X49_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBN4795422.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            11420..11692
                     /locus_tag="L2X49_000272"
     CDS             11420..11692
                     /locus_tag="L2X49_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBN4795423.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            11919..12215
                     /gene="ghoS"
                     /locus_tag="L2X49_000273"
     CDS             11919..12215
                     /gene="ghoS"
                     /locus_tag="L2X49_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBN4795424.1"
                     /translation="MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            12243..12416
                     /gene="ghoT"
                     /locus_tag="L2X49_000274"
     CDS             12243..12416
                     /gene="ghoT"
                     /locus_tag="L2X49_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HBN4795425.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(12535..14052)
                     /gene="lysS"
                     /locus_tag="L2X49_000275"
     CDS             complement(12535..14052)
                     /gene="lysS"
                     /locus_tag="L2X49_000275"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBN4795426.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYNEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(14289..15746)
                     /gene="dtpC"
                     /locus_tag="L2X49_000276"
     CDS             complement(14289..15746)
                     /gene="dtpC"
                     /locus_tag="L2X49_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HBN4795427.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(15805..17952)
                     /gene="cadA"
                     /locus_tag="L2X49_000277"
     CDS             complement(15805..17952)
                     /gene="cadA"
                     /locus_tag="L2X49_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBN4795428.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(18032..19366)
                     /gene="cadB"
                     /locus_tag="L2X49_000278"
     CDS             complement(18032..19366)
                     /gene="cadB"
                     /locus_tag="L2X49_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBN4795429.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(19732..21270)
                     /gene="cadC"
                     /locus_tag="L2X49_000279"
     CDS             complement(19732..21270)
                     /gene="cadC"
                     /locus_tag="L2X49_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HBN4795430.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(21887..21962)
                     /locus_tag="L2X49_000280"
     tRNA            complement(21887..21962)
                     /locus_tag="L2X49_000280"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21927..21929),aa:Phe,seq:gaa)
     gene            complement(22069..22644)
                     /locus_tag="L2X49_000281"
     CDS             complement(22069..22644)
                     /locus_tag="L2X49_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN4795431.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(22681..24378)
                     /gene="dsbD"
                     /locus_tag="L2X49_000282"
     CDS             complement(22681..24378)
                     /gene="dsbD"
                     /locus_tag="L2X49_000282"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBN4795432.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(24354..24692)
                     /gene="cutA"
                     /locus_tag="L2X49_000283"
     CDS             complement(24354..24692)
                     /gene="cutA"
                     /locus_tag="L2X49_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBN4795433.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(24808..26109)
                     /gene="dcuA"
                     /locus_tag="L2X49_000284"
     CDS             complement(24808..26109)
                     /gene="dcuA"
                     /locus_tag="L2X49_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBN4795434.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(26227..27663)
                     /gene="aspA"
                     /locus_tag="L2X49_000285"
     CDS             complement(26227..27663)
                     /gene="aspA"
                     /locus_tag="L2X49_000285"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBN4795435.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            28000..28476
                     /gene="fxsA"
                     /locus_tag="L2X49_000286"
     CDS             28000..28476
                     /gene="fxsA"
                     /locus_tag="L2X49_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBN4795436.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(28492..29748)
                     /gene="yjeH"
                     /locus_tag="L2X49_000287"
     CDS             complement(28492..29748)
                     /gene="yjeH"
                     /locus_tag="L2X49_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBN4795437.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            30024..30317
                     /locus_tag="L2X49_000288"
     CDS             30024..30317
                     /locus_tag="L2X49_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBN4795438.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            30361..32007
                     /gene="groL"
                     /locus_tag="L2X49_000289"
     CDS             30361..32007
                     /gene="groL"
                     /locus_tag="L2X49_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBN4795439.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            32145..32498
                     /locus_tag="L2X49_000290"
     CDS             32145..32498
                     /locus_tag="L2X49_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBN4795440.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(32691..33560)
                     /locus_tag="L2X49_000291"
     CDS             complement(32691..33560)
                     /locus_tag="L2X49_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBN4795441.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(33955..34983)
                     /gene="epmB"
                     /locus_tag="L2X49_000292"
     CDS             complement(33955..34983)
                     /gene="epmB"
                     /locus_tag="L2X49_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBN4795442.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            35025..35591
                     /gene="efp"
                     /locus_tag="L2X49_000293"
     CDS             35025..35591
                     /gene="efp"
                     /locus_tag="L2X49_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBN4795443.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            35643..35768
                     /gene="ecnA"
                     /locus_tag="L2X49_000294"
     CDS             35643..35768
                     /gene="ecnA"
                     /locus_tag="L2X49_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBN4795444.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            35879..36025
                     /gene="ecnB"
                     /locus_tag="L2X49_000295"
     CDS             35879..36025
                     /gene="ecnB"
                     /locus_tag="L2X49_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBN4795445.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            36207..36524
                     /gene="sugE"
                     /locus_tag="L2X49_000296"
     CDS             36207..36524
                     /gene="sugE"
                     /locus_tag="L2X49_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBN4795446.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(36521..37054)
                     /gene="blc"
                     /locus_tag="L2X49_000297"
     CDS             complement(36521..37054)
                     /gene="blc"
                     /locus_tag="L2X49_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBN4795447.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(37143..38276)
                     /locus_tag="L2X49_000298"
     CDS             complement(37143..38276)
                     /locus_tag="L2X49_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBN4795448.1"
                     /translation="MFKTTLCALLITASCSTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNALQ"
     gene            complement(38339..38698)
                     /gene="frdD"
                     /locus_tag="L2X49_000299"
     CDS             complement(38339..38698)
                     /gene="frdD"
                     /locus_tag="L2X49_000299"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBN4795449.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(38709..39104)
                     /gene="frdC"
                     /locus_tag="L2X49_000300"
     CDS             complement(38709..39104)
                     /gene="frdC"
                     /locus_tag="L2X49_000300"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBN4795450.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(39115..39849)
                     /gene="frdB"
                     /locus_tag="L2X49_000301"
     CDS             complement(39115..39849)
                     /gene="frdB"
                     /locus_tag="L2X49_000301"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBN4795451.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(39842..41650)
                     /gene="frdA"
                     /locus_tag="L2X49_000302"
     CDS             complement(39842..41650)
                     /gene="frdA"
                     /locus_tag="L2X49_000302"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBN4795452.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            41975..42952
                     /gene="epmA"
                     /locus_tag="L2X49_000303"
     CDS             41975..42952
                     /gene="epmA"
                     /locus_tag="L2X49_000303"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBN4795453.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            43171..44673
                     /gene="yjeM"
                     /locus_tag="L2X49_000304"
     CDS             43171..44673
                     /gene="yjeM"
                     /locus_tag="L2X49_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBN4795454.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            44725..45039
                     /locus_tag="L2X49_000305"
     CDS             44725..45039
                     /locus_tag="L2X49_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795455.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            45036..45350
                     /locus_tag="L2X49_000306"
     CDS             45036..45350
                     /locus_tag="L2X49_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HBN4795456.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(45379..48702)
                     /gene="mscM"
                     /locus_tag="L2X49_000307"
     CDS             complement(45379..48702)
                     /gene="mscM"
                     /locus_tag="L2X49_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBN4795457.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(48724..49692)
                     /gene="psd"
                     /locus_tag="L2X49_000308"
     CDS             complement(48724..49692)
                     /gene="psd"
                     /locus_tag="L2X49_000308"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBN4795458.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(49789..50841)
                     /gene="rsgA"
                     /locus_tag="L2X49_000309"
     CDS             complement(49789..50841)
                     /gene="rsgA"
                     /locus_tag="L2X49_000309"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBN4795459.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            50936..51481
                     /gene="orn"
                     /locus_tag="L2X49_000310"
     CDS             50936..51481
                     /gene="orn"
                     /locus_tag="L2X49_000310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBN4795460.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            51692..51767
                     /locus_tag="L2X49_000311"
     tRNA            51692..51767
                     /locus_tag="L2X49_000311"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51725..51727,aa:Gly,seq:gcc)
     gene            51925..52000
                     /locus_tag="L2X49_000312"
     tRNA            51925..52000
                     /locus_tag="L2X49_000312"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51958..51960,aa:Gly,seq:gcc)
     gene            52036..52111
                     /locus_tag="L2X49_000313"
     tRNA            52036..52111
                     /locus_tag="L2X49_000313"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52069..52071,aa:Gly,seq:gcc)
     gene            52345..52398
                     /locus_tag="L2X49_000314"
     CDS             52345..52398
                     /locus_tag="L2X49_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795461.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(52381..53520)
                     /gene="queG"
                     /locus_tag="L2X49_000315"
     CDS             complement(52381..53520)
                     /gene="queG"
                     /locus_tag="L2X49_000315"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBN4795462.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            53519..55066
                     /gene="nnr"
                     /locus_tag="L2X49_000316"
     CDS             53519..55066
                     /gene="nnr"
                     /locus_tag="L2X49_000316"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBN4795463.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYDGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            55038..55499
                     /gene="tsaE"
                     /locus_tag="L2X49_000317"
     CDS             55038..55499
                     /gene="tsaE"
                     /locus_tag="L2X49_000317"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBN4795464.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            55518..56855
                     /gene="amiB"
                     /locus_tag="L2X49_000318"
     CDS             55518..56855
                     /gene="amiB"
                     /locus_tag="L2X49_000318"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBN4795465.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            56865..58712
                     /gene="mutL"
                     /locus_tag="L2X49_000319"
     CDS             56865..58712
                     /gene="mutL"
                     /locus_tag="L2X49_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBN4795466.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            58705..59655
                     /gene="miaA"
                     /locus_tag="L2X49_000320"
     CDS             58705..59655
                     /gene="miaA"
                     /locus_tag="L2X49_000320"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBN4795467.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            59741..60049
                     /gene="hfq"
                     /locus_tag="L2X49_000321"
     CDS             59741..60049
                     /gene="hfq"
                     /locus_tag="L2X49_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBN4795468.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            60125..61405
                     /gene="hflX"
                     /locus_tag="L2X49_000322"
     CDS             60125..61405
                     /gene="hflX"
                     /locus_tag="L2X49_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBN4795469.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            61491..62750
                     /gene="hflK"
                     /locus_tag="L2X49_000323"
     CDS             61491..62750
                     /gene="hflK"
                     /locus_tag="L2X49_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBN4795470.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            62753..63757
                     /gene="hflC"
                     /locus_tag="L2X49_000324"
     CDS             62753..63757
                     /gene="hflC"
                     /locus_tag="L2X49_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBN4795471.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            63839..64036
                     /locus_tag="L2X49_000325"
     CDS             63839..64036
                     /locus_tag="L2X49_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBN4795472.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            64140..65438
                     /gene="purA"
                     /locus_tag="L2X49_000326"
     CDS             64140..65438
                     /gene="purA"
                     /locus_tag="L2X49_000326"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBN4795473.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            65643..66068
                     /gene="nsrR"
                     /locus_tag="L2X49_000327"
     CDS             65643..66068
                     /gene="nsrR"
                     /locus_tag="L2X49_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBN4795474.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            66107..68548
                     /gene="rnr"
                     /locus_tag="L2X49_000328"
     CDS             66107..68548
                     /gene="rnr"
                     /locus_tag="L2X49_000328"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBN4795475.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            68728..69459
                     /gene="rlmB"
                     /locus_tag="L2X49_000329"
     CDS             68728..69459
                     /gene="rlmB"
                     /locus_tag="L2X49_000329"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBN4795476.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT        17082 a        17146 c        18208 g        17068 t
ORIGIN      
        1 actgcttatt atcgacggct aaactatttt tttggctgat actgatatcg tctttagccg
       61 ggaaacgtct ggcggcgctg ttggctaagt ttgcggtatt gttgcggcga catgccgaca
      121 tatttgccga acgtgctgta aaaacgacta cttgaacgaa agcctgccgt cagggcaata
      181 tcgagaatac ttttatcggt atcgctcagt aacgcgcgaa cgtggttgat gcgcatcgcg
      241 gtaatgtact gtttcatcgt caattgcatg acccgctgga atatccccat tgcatagttg
      301 gcgttaagtt tgacgtgctc agccacatcg ttgatggtca gcgcctgatc atagttttcg
      361 gcaataaagc ccagcatctg gctaacataa aattgcgcat ggcgcgagac gctgtttttg
      421 tgtgtgcgcg aggttttatt gaccagaatc ggttcccagc cagagaggct aaatcgcttg
      481 agcatcaggc caatttcatc aatggcgagc tggcgaattt gctcgttcgg actgtttaat
      541 tcctgctgcc agcggcgcac ttcaaacggg ctaagttgct gtgtggccag tgatttgatc
      601 accatgccgt gagtgacgtg gttaatcagg tctttatcca gcggccagga gagaaacaga
      661 tgcatcggca gattaaaaat cgccatgctc tgacaggttc cggtatctgt tagttggtgc
      721 ggtgtacagg cccagaacag cgtgatatga ccctgattga tattcacttt ttcattgttg
      781 atcaggtatt ccacatcgcc atcgaaaggc acattcactt cgacctgacc atgccagtgg
      841 ctggtgggca tgatatgcgg tgcgcgaaac tcaatctcca tccgctggta ttccgaatac
      901 agcgacagcg ggctgcgggt ctgtttttcg tcgctgctgc acataaacgt atctgtattc
      961 atggatggct ctctttcctg gaatatcaga attatggcag gagtgaggga ggatgactgc
     1021 gagtgggagc acggttttca ccctcttccc agagaggcga ggggagcgtc cgagcatcat
     1081 gaggccgaaa actctgcttt tcaggtaact tattaccata aactcagatt tactgctgct
     1141 tcacgcagga tctgagttta tgggaatgct caacctggaa gccggaggtt ttctgcagat
     1201 tcgcctgcca tgatgaagtt attcaagcaa gccaggagat ctgcatgatg actgcaccca
     1261 aaattacatt tatcggcgct ggttcgacga ttttcgttaa aaatattctt ggtgatgtgt
     1321 tccatcgcga ggcgctgaaa acggcgcata ttgccctgat ggacattgat cccacccgcc
     1381 tggaagagtc gcatattgtg gtgcgtaagc ttatggattc agcaggggcc agcggcaaaa
     1441 tcacctgcca cacccaacag aaagaagcct tacatgatgc cgattttgtc gtggtggcat
     1501 ttcagattgg cggttatgaa ccttgcacgg tgactgattt cgaggtctgt aagcggcatg
     1561 gtctggaaca aaccattgcc gatacgttgg ggccgggcgg tattatgcgc gcgctacgta
     1621 ccattccgca tctgtggcaa atttgcgagg acatgacgga agtctgcccc gatgccacca
     1681 tgctcaacta tgttaaccca atggcgatga atacctgggc gatgtatgcc cgctatccgc
     1741 atatcaaaca ggtcgggctg tgccattcgg tgcagggaac ggcggaagag ctggcgcgtg
     1801 acctcaatat cgacccagct acgctgcgtt accgttgcgc aggtatcaac catatggcgt
     1861 tttacctgga gctggagcgc aaaaccgccg acggcagtta tgtgaatctc tacccggaac
     1921 tgctggcggc ttatgaagca gggcaggcac cgaagccgaa tattcatggc aatactcgct
     1981 gccagaatat tgtgcgctac gaaatgttca aaaagctggg ctacttcgtc acggaatcgt
     2041 cagaacattt tgctgagtac acaccgtggt ttattaagcc aggtcgtgag gatttgattg
     2101 agcgttataa agtaccgctg gatgagtacc cgaaacgctg cgtcgagcag ctggcgaact
     2161 ggcataaaga gctggaggag tataaaaacg cctcccggat tgatattaaa ccgtcacggg
     2221 aatatgccag cacaatcatg aacgctatct ggactggcga gccgagtgtg atttacggca
     2281 acgtccgtaa cgatggtttg attgataacc tgccacaagg atgttgcgtg gaagtagcct
     2341 gtctggttga tgctaatggc attcagccga ccaaagtcgg tacgctacct tcgcatctgg
     2401 ccgccctgat gcaaaccaac atcaacgtac agacgctgct gaccgaagct attcttacgg
     2461 aaaatcgcga ccgtgtttac cacgccgcga tgatggaccc gcatactgct gccgtgctgg
     2521 gcattgatga aatatatgct cttgttgacg acctgattgc cgcccacggc gactggctgc
     2581 caggctggtt gcaccgttaa aacgcgacta aacgctactg cgccggggga tttattccgg
     2641 cgcacacctc tgacgatacc aataacagaa ggcgggcgtt ggtaacagcg acccgatacc
     2701 ctatgagcat ttcaatgact acaaaactca gttatggatt tggagcgttc gggaaggatt
     2761 ttgcgatcgg cattgtgtat atgtacctca tgtattacta caccgatgtc gttgggctgt
     2821 ctgtgggttt ggtcggtact ttgtttctgg tggcgaggat ctgggatgct attaacgatc
     2881 cgattatggg atggattgta aatgctacgc gatcgcgatg gggtaagttc aaaccctgga
     2941 tcctgatcgg tacgttggca aactctgtaa tcttatttct cctctttagt gcgcatctgt
     3001 ttgaaggtac tactcagatt gtctttgttt gcgtgaccta catcctctgg ggcatgactt
     3061 acaccattat ggatattccc ttctggtcgc tggttccaac catcacgctc gataaacgtg
     3121 agcgctaaca actggttcct tatccgcgtt tttttgccag tctggcaggc tttgttacgg
     3181 caggtgtgac gctaccattt gttaattatg tcggcggtgg cgatcgggga tttggctttc
     3241 agatgttcac tctggtacta atcgcctttt ttattgtttc aaccatcatc actctgcgca
     3301 atgtgcatga agtcttttcg tcagacaatc aaccgtctgc tgaaggaagc catctgacac
     3361 ttaaagccat cgttgcgcta atttataaaa acgatcagct ttcatgcctc ttgggtatgg
     3421 ctcttgctta taatgtagcc agcaacatta ttaccggatt tgctatctat tatttctcat
     3481 atgttatcgg tgatgcggat ttgttcccct attatctgtc gtatgcggga gctgctaacc
     3541 tggtgacgtt agtattcttc ccacgcttag ttaaatcatt atcccgacgc attttatggg
     3601 ccggagcatc tattcttccg gtgttaagct gtggtgttct cctgttaatg gcattaatga
     3661 gctatcacaa cgtcgtcctc attgtgattg cgggtatttt gctgaatgtg ggaacggcgc
     3721 ttttctgggt attacaggtc atcatggtgg cagataccgt tgattacggt gaatataaac
     3781 tgcacgtacg ctgtgaaagt atcgcttact ccgtgcagac tatggtggtg aagggcggtt
     3841 cagcctttgc ggcttttttc attgcggttg tgttagggat gattggctat gtaccgaatg
     3901 ttgaacagtc tacgcaagcc ctattaggta tgcagtttat tatgattgct ctaccaactc
     3961 tgtttttcat ggtaacgctg attctctact tccgtttcta tcgcctcaat ggcgacacgc
     4021 tgcgcaggat ccagattcat ctgctggata aatatcgcaa agtaccgccc gagcctgttc
     4081 atgctgatat tccggtcggt gcagtgagtg atgtgaaagc ctgacgtgga caatagctaa
     4141 cgaaaatggc ctgatgcgat atgtttatca ggccacgtct taatgtaatg ctttggattt
     4201 gcaaattttg tagaccggat aaggaattca cgccgcatcc ggcatcaaca aagcgcacgt
     4261 tgttatccgg ttatcaagcc aaagcgccgt agctggcggc aatgaaagcg agcgaggaat
     4321 atcaccgtcg ttaatgcgcc aagcagcgcg cagaacatat cggattgcgt gtcccactgg
     4381 tcgccctggg tgccgagaaa atcatccgct ccctggccca ttgccagcgc cgcccaccac
     4441 tcgattaatt catacatcgc gcttatcgcc agcgccacgc agcagaccag gaacgccacc
     4501 attttacgtc cgcgcacgta catcccgcgc acgagaattt ctcgtgccac cagtgcaggc
     4561 accagcccct ggaaaaaatg ccccagcttg tcatacggat tacggctcaa ccccagccat
     4621 tcctgcacct caaaaccgat ggggactttc gcgtaggtgt attgcccgcc gaccatcagg
     4681 atgatggcgt gaaagaaaat gagcgtatag agcagcggcg ttaacggata acgtttagcg
     4741 gtggcaagca gtagcggcac gacaataatc accggtgtca cttccatcag ccaggtgagt
     4801 ttgtcatggg ccgaaatgcc ggtataaatc aggattaacg ttagcgccag tgcgccggtg
     4861 ttaagaatta acggcttgag tgtgcgggtc atggtgagtt cgcaagtcag ggaaaatact
     4921 gactattcac cagcaagcgt gaaattacaa tcgcaaaaga ggtggcccag ggggatcacc
     4981 tggcagcatg ctgccaggcg ctgggccgaa gaggttactt agtgcagttc gcgcactgtt
     5041 tgttgacgat ctgctggaag aagtcgttac ctttgtcatc gaccaggata aacgccggga
     5101 aatcttctac ttcgattttc cagatagctt ccatacccag ctccggataa gcgacgcatt
     5161 ccagatgctt gatgctctgc tgcgccagta ccgccgccgg accgccgatg ctaccgaggt
     5221 agaagccgcc gtgtttatga cacgcgtcgg taacctgctg actgcggtta cctttcgcca
     5281 gcatgatcat gctgccgccg tgggattgca gcagatccac gtaggagtcc atacggcctg
     5341 cggtggttgg gccaagtgaa cctgatggat aaccggcagg ggttttcgcc ggacccgcgt
     5401 agtagatcgg gtgatctttg atgtactgcg gaagttcttt accggcgtca atcagctctt
     5461 tcagcttggc gtgtgcaata tctcggccca cgataatggt gccggtgagc gacaaacgag
     5521 tggataccgg gtattgcgaa agctgggcga ggatctcttt catcgggcgg ttaaggtcaa
     5581 ctttcaccgc ttcgccttca ccggcctggc gcagttcttg tggaatgtac tggcctgggt
     5641 tgtgttccag tttttcgatc cagatacctt cgcggttgat tttcgcttta atgttacggt
     5701 cagcggagca ggagacgccc atgccgaccg ggcaggatgc gccgtgacgt ggcagacgga
     5761 taacgcgaat gtcgtgcgcg aagtatttac cgccaaactg cgcgccaaga ccgagtttct
     5821 gggcctcttc cagcagttcc tgttccagct ggacatcgcg gaacgcctga ccatgttcgt
     5881 tcccttccgt cggcagttca tcgtaatagt gagcgcttgc taacttgact gttttcaggt
     5941 tggtttccgc agacgtaccg ccaatcacaa acgcgatatg gtacggcggg caggctgcag
     6001 taccgagggt acgcattttc tcgacgagga agtttttcag tttgccggga gtcagcaggg
     6061 ctttggtttc ctggtagaga tacgttttgt tggcagagcc gccgcctttc gcaacgcaaa
     6121 ggaatttgta ctcatcgcca tctaccgcgt acaggtcgat ttgcgcaggc aggttagtgc
     6181 cggtgttgac ctctttgtac atgtccagcg ccgcattctg tgaatagcgc aggttatctt
     6241 cgatataggt gttatagacg cctttcgaca gcgcttcttc atcaccgccg ccggtccaca
     6301 cgcgctggcc ttttttaccg acgatgatcg cggtgccggt atcctggcag gtcggcagca
     6361 cgcctttggc ggcgatttcg gagtttctta agaattgcag cgccacgtac ttgtcgtttt
     6421 cgctggcttc tggatcgtga agaatagccg caacctgttt ctggtgtgcc gggcggagca
     6481 taaaagaagc gtcgtgaaag gcttgctgcg ccagcagggt cagggcttct ggttccactt
     6541 tcaggatggt ttcgccgtcg aagtcggcaa cgctaacgta atcggaagtg agtagatagt
     6601 attcggtatt gtctttcccc atcgggaaag gtgcctggta gataaagggt ttgtttgaca
     6661 tagcttccag cctgtaactc tgtatttgtt attcgaaaat ggcgtgccgc gtagtcacgg
     6721 gcggcacgca aagtgcattt ataagaaccc gtacatcgcg gcgaagatcc agccgaagac
     6781 gcacgatacg ctcacaccaa tcaaccccgg cagaataaag ctgtggttga tgacgaagcg
     6841 accgatgtgg gtggtgccgg aacggtcaaa ctgaatcgct gccagatcgc tcggataagt
     6901 cggcaggatg taataaccgt agcaagccgg tgctgaagcc acgatgtatg ccggatcaac
     6961 gccgatcgcc agcgcgaccg gaacaatcgc cgccagcgcc gcagcctgag agtttacaaa
     7021 cttggaaacc agcagcagaa caatggcata ggcccacgga tactctttca ccatttcacc
     7081 cagtacgccc tgaatttcag acatatgcgc accgaacatg gtttctgcca tccatgcgat
     7141 accgtacacc gccacgatgg cgatcatacc ggaacggaag acttcgtttt ttgagataga
     7201 cgcgggattg gttttggtca ggataataat cagcgccccg gtcagcagca taaacatctg
     7261 aataaccagt accatcgaca gcggtttgcc gccgaaggat ggacgcaggt ccgaatcagc
     7321 accaagaagg gcgactacag cgattgcacc gaggaaaatc cacattgcca gccagttgct
     7381 tttcggcagt tttttatcga gcagcgtcgc ggtatcaccg taaacatact cacggttttc
     7441 cggtacggag atgaatttct ggaactcttc gtctttatcc agatctttac cgcggaacca
     7501 gctgaagata ccgatcgcca ggataccgat taacgtcgat ggaatggtga ttgccagcag
     7561 gtcgaggaac tcaagatggc gaccatcaaa ggtgacatta cccagcattg caaccagaga
     7621 cacgaccgca accgacaccg gactggcgat aatccccatc tgtgcaccga tagaacttgc
     7681 cgccatcgga cgttccggac ggatgttgtt cttaatggcg acgtcgtaga tgatcggcag
     7741 aatggtgtaa accacatgac ccgtaccgca aagaatggtc agtgtacagg tcacaaacgg
     7801 cgcgacaatt gagacatatt tcgggttgcg gcgcagcaac ttctcggcaa tttgcagcat
     7861 gacatcaaga ccgcccgaag cttgcaaggt cgccgatgcc gccaccaccg caatgataac
     7921 cagcatgaca tcaactggtg gtttacctgg ctgaaggtgg aagacgaaga ccagaatgac
     7981 cagaccgata ccgcctaata aacccagcgc gataccaccc tttctggcac cataaaacag
     8041 acatatcagt attatgataa gttggatagt aaataacatg tgtgaaccct cgcgataatc
     8101 ctatttaaat ttttgctgaa tagatcacag tcacgttctg ttttgtatga actgttttca
     8161 gagcctgact aaatatccgt ttggtcgtta ccggctttag caaatacctc acagtaaata
     8221 ttggctgatt gaagcagcgg caaatttatg cactgtgata agcggctttt tcagagagaa
     8281 aaactctctg gcgatgggtt tatagtttgt actgcgcaga tgttaatgtt ttgtttgctg
     8341 ttatcttatt gatatttatc ggtctatttt ttgttctcag ggcagatatc attaggttta
     8401 aagattaatc tgatctaccc atttgtgggt aaaaatacac ataatgcggg tgacataata
     8461 gttaattaac ttttgttagc gttttgaaat taaaaacacc gttcacctga agagatatta
     8521 atttttagcg atgatggagg gataattata tttgatctgg cacaagtttt actgatgaag
     8581 gatgtaactt gtgccagggg tattttgcat tacggtaata attattactt acagataacc
     8641 agcgatccgt tattggcaat attgtttcag tagtgagtag tgttctgcct gaatacggta
     8701 acggtaaacc ggacgccccg tgacgccata atggatactg gtgaacaaga tgtggcagtt
     8761 gaccagccag atgaggtatt tacggcagga aacacgcgag atgttaacct cgttagccag
     8821 ctcgtcggtt gaaaactcat agtcctgatg cgcgtcaatc cactggcaca gcgtgcgtaa
     8881 cgtctgcggc gttaagcctt ttggcaagcg acgaggatcc tgttcgttgg agctgctgcc
     8941 gtggattagc tgatcaagct cggcctggtc ataatactga tgtttttcca gcgccatttt
     9001 cttttgccgc cagccggtga gcgcctcttc aaagcgggaa gcctggaagg gtttgatcag
     9061 gtaatccacg acaccgtaat gcagcgaatc tttaatggtt gccgcatcgg ctgcggagga
     9121 gatgacaatc acatcacttt tgcaacgcgc gttatgcagg acaggcagta aatcgagccc
     9181 gttctctttt tgcatataga tatcgagcaa tatcaggtcg ataggcgtat cgctattgaa
     9241 gataatctct ttggctttct ccagcgtcga ggctgttcca cagcattgaa agcctgggat
     9301 ttgtgctacg tatcggcgat tcagctccgc gaccattgcg tcgtcatcga taattaatac
     9361 attgatcatc tgttcgacct ctccccgtcc cagggtatct ggacaaaaaa ttgtgtgaaa
     9421 atcccgggtt ccgattccac ggcgatgctg ccgccgagat tttctacctg ttgtttgaca
     9481 agtgctaaac cgacgcctcg ctcgcttcct tttgtcgaga cacctttgtc aaaaatgtga
     9541 tcgattttgt cgggtgcgat ccccggtcca tcatcgttaa cttcgcagtg cagccagccg
     9601 tgacggtagt gcaatgttac gctaatttcg ccaccgggtt ccggccctaa tgcttccagc
     9661 gcgttttcta tcagatttcc caatgtggta atcagcgtcg cgacctggtc ctcactgccg
     9721 ctgtctggca gctggctttc actgtttaaa atcagcgtat ggcctaaatc ggtcgcgcga
     9781 ttaatcttgc tgattaaaaa accagcgata accggagatt tgatcttacc cagcagagag
     9841 ccaatctctt cctgatagtt attggctgtt ttgagaatgt aatcttccaa ctgcttataa
     9901 ctcttcagat gcaataatcc gagaatcaca tgcaatttat tcataaattc gtgggatcgt
     9961 tcacgaagtg catcagcata gttgaccaga ccgtcgagtc gctgcatcag tttacgaact
    10021 tcagttttgt ccctgaaggt tgaaatggca ccgatgataa cgccattact gcgcaccgga
    10081 acggtgttga tcagtaatag ccggtcttta atcgtaatct cttcgtcgcg gcgcggcgta
    10141 ccgtcgcgta acacttccga gacatctacc acctgtgacc atgagtggct gagcgtcgac
    10201 agtttctcat cgtcctgcga cttacggtaa ttcagcaatt cttgtgcggc atcgttgatc
    10261 agcgtgacct cgccgcgatc gtccacggca acgacgcctt ctttgataga ctgcaacatg
    10321 gcctggcgct gctcaaacag cgtggagatt tcgtagggtt ccaggccgaa aaggattttt
    10381 ttcagtacct taaccagaat gcaggtgcca atcagtccga ccagcatgcc aaataatacc
    10441 gaccagataa tgctccagcg actgtcattg atttgttggg tcacgcggct taactcaagg
    10501 ccgatcgcca ccacgccaat ttgtttatga ttttcatcgt agatgggggt aaatacgcgt
    10561 aaagcctgcg ccagaaaacc gcgattgata gcgacatttt ctttgccatt cagcgcttta
    10621 aggatgtcat cacctttaaa tggctgacca atacgctggg cttcaggatg cgagtagcga
    10681 agactttgca tatcggtaac gacaataaac agcagatcgt tgcgtttgcg cacggcttcc
    10741 gcgatggcct ggattccgct ctcctgcggt tttttctgca agccctgacg gatttccggc
    10801 gagtcggcga gggtacgcgc cactgccagt gccttgttgg ccagcccgtc tcgcgtcata
    10861 tcactgattt gcgagaagta aatcagatgc accaccaata gcaccgagaa cagtaccgcg
    10921 ctgaccatta agatcactgt ggtactcaat ttcatcggac gtttgcgtaa catgcggtag
    10981 ggcaatgaat gtctcatcag cttccttgtg tgacaaattt cttaagcatt atctctgatg
    11041 aggcgggtaa ttcaaaggga gtaagaatga ttggctatat aggggaagag actctggcaa
    11101 cggaaactgc cagtgctgta tgaagattcc agggctatgc ttatagcgat aatcatactg
    11161 atgagagagg gaaagtcatg gatcaggcgc tactggacga gggttatcgc tgttataccg
    11221 gcgaaaagat cgatgtctat ttcaacactg cgatatgtca gcattctggc aattgcgtac
    11281 gtggcaacgg caagttattt aatctcaaac gaaagccgtg gatcatgccg gatgaagtcg
    11341 acgtcgtcac tgtggttaaa gtgattgata cgtgcccgag cggcgcgctg aaataccgtc
    11401 ataaataagc gagggtaaaa tggaaatacg cgaaggccac aataaatttt acattaatga
    11461 tgaacagggc aagcaaatcg ctgaaattgt ctttgtgccg accggagaga atttagcgat
    11521 tatcgaacat accgatgtcg atgaaagcct gaaagggcaa gggattggta aacagctggt
    11581 tgcgaaagtc gtggaaaaaa tgcgtcggga aaaacgaaaa attatcccat tatgtccatt
    11641 tgcgaaacac gaatttgata aaacgcggga gtatgatgat attcgcagtt gatgggagag
    11701 tacagattca cgatattttt catgctctct gcggtgtgat gcaggagagc atctgaaggg
    11761 tagggggatg cacaaagaat gggcagagag cgcgtttttt tgtcccaagt catccccttt
    11821 actgagcaaa aaaaagaata tctcctatat gagaatcatc aatcggggtt aataagtttt
    11881 gcgtccccag agtgttcaat attgatagga gtcatattat ggaaggtaaa aacaagttca
    11941 atacttatgt tgtttctttt aattatccat catcttattc ctcagtgttc ttaaggttaa
    12001 gatcattgat gtatgatatg aatttctcct ctatcgtggc tgatgaatat gggataccac
    12061 gacaattgaa tgaaaactcc ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag
    12121 atttaatcag gcttaaatgc ttagacttac cggatattga ctttgacctc aacattatga
    12181 cagttgatga ctatttccgt cagttttaca agtagccagg aaggataaga agaacataaa
    12241 aaatggcact attctctaaa atattaattt tttatgtgat tggtgtgaac atatcctttg
    12301 tcattatctg gtttatctca catgagaaaa cacatattcg tttacttagt gcattcctgg
    12361 tcggaataac ctggccaatg agtctgcctg tggcattact tttttctctc ttttaggagc
    12421 tgtagttgag tggacccgct ggttatcgtg aaacgaagcg tctctcctca aaactggatt
    12481 tgcggaacga tgaacgaagc agtcgcctga ctgcttcgtt cattaaagtg aaatttattt
    12541 ctgtgggcgc atcgccggga agagaataac gtcacgaata gtatgactgt ttgtaaacag
    12601 cataatcatt cggtcgatgc caatacccag accagcggtt ggcggcagac catattccag
    12661 cgcagtcacg taatcttcgt tatagaacat ggcttcgtcg tcacctgcag ctttagcgtt
    12721 aacctgttcc tggaaacgtt cagcctgatc ttctgcgtcg tttaattcgc taaaaccatt
    12781 accgatttca cgaccaccga tgaagaattc aaaacggtcg gtgatttccg ggttaacatc
    12841 attacggcgc gccagcgggg acacttctgc cggatattcc gtaataaagg ttggctgaat
    12901 cagatgtgct tctgccactt catcaaagat ctccgtgaca atacgtccca gcccccagct
    12961 tttctctacc gtaattccga tagattcagc taatgcttta gcagcatcaa aattatccag
    13021 gtcggccata tcggtttctg gacgatattt tttgattgct tcgcgcatgg tgagtttttc
    13081 aaacggtttg ccgaaatcaa acacatgctc gccataagtg actttagtgg tacccagaac
    13141 ctcttgtgcc agggtgcgga acagtgactc tgtcagttca atcaaatcgt ggtaatccgc
    13201 atacgccatg tagagttcca tcattgtgaa ctcaggatta tggcgaacag aaataccttc
    13261 attacggaag ttacggttga tttcgaatac ccgttcaaaa ccgcctacaa ccagacgttt
    13321 cagatacagc tccggcgcga tacgcagata catatctaaa tccagagcat tatgatgggt
    13381 aataaacggg cgagcagatg ccccacctgg aattacctgc atcatcgggg tttctacttc
    13441 cataaagccg cgcgcgacca tgaactggcg gatagcggcc agaatttttg aacggacaac
    13501 aaacgtttga cgggatttat cgttagcgat gaggtccaga taacgttgac gataacggac
    13561 ttcctgatcc tgcagaccat ggaatttatc tggtaaagga cgtagtgctt ttgtcagcag
    13621 gcgcaactca gtacagtgaa tggaaagctc accggtttgc gtcttaaaca gcgtaccgcg
    13681 ggcaccgata atgtcaccca gatcccattt tttaaactga tcgttataaa caccttctgg
    13741 caggctatct cttgcaacgt acagttgaat acggccaccg acatcctgca acgttacaaa
    13801 ggaggctttc cccatgatac gacgggtcat cattcggcca gcaaccgaga cttcaatgtt
    13861 taaggattcc agttcctggt tatccttcgc atcaaactct tcgtgcagct ggtcagaggt
    13921 atggtcgcgg cgaaaatcat tgggaaacgc cacaccttgc tgacgtagtg ccgccagttt
    13981 ttcgcggcga tttctcagtt catcgttaaa atcaatagcc tcattggctc cccgtgtttc
    14041 ttgttcagac attttggttc ctctaaatcc agctttcaat ttagcgtaga taaagagaca
    14101 gatcggtaca ctgtattatc tgcctcgact attaataact cagtttatct tatcgaaact
    14161 aacgcttacc tcaagttgat atcactaata aataaccatc aaaatggttt aaatgactta
    14221 ttaatctaga tttgctagtg ttataaactg aaagttaatt tattcataac caattgtttt
    14281 tacgacagtt aatcgttgct ctcctgtatc atattcgtcg gcgtgctaaa cagaaaacgg
    14341 gtagcaaagg ccaatacgac gatgatcgcg acacaagcca acgtccactc tcccatctga
    14401 gaaaagaatc gctggtaagc tgcaattgcc ataccgctaa tttgcgactc tgtcgtctgc
    14461 tgtgccacga cgcctgccag ccagttggcg accgcgcctg ttgccagcat ataaataccg
    14521 gttaatacgc cagacatttt cagacgcgtg atttgcgcaa tcgccaccgg gtcaataaag
    14581 agttcggcaa agcccattag cgccaacccg gatatcatca cgcccattga cgcttgaccg
    14641 tcagccgctg catgtcgggc atcaaatgcc aacaacataa agccacaagc catcagcagt
    14701 aagccaaagg caaacttcag ccagacgcgc aatgttgagt tgccgcggct ttctggcgac
    14761 gccagccagg ccagtacaac cccagcgagc atcaccgcaa ttgcattcac cgactggaat
    14821 agtgctgtag gtacttcaat attgaatgcc tgacgattca caaagcgatc gataaacaag
    14881 ctgatggtac tgccgccctg ttgtgccagt acccagaaca atgtcccgac aaacatcaac
    14941 aatacaattt gccaaagagc acggcggtgt tcggggaatt tgaccatcat gcgggcaatg
    15001 atttgtgcgg caatgaggca aacgatcgcc agcaaatatc cggaccagtc gttctccagc
    15061 agcagggtaa aaaatactgg ggctaaacag agcatcacca ctaaccagct ccataccggt
    15121 aaggcaaatt tgacgctagt gagcgctttt ttatccatac tacgcgtgga ctggaaatga
    15181 cgatgcccgc ttaagaaaat caacaaaccg ataaacatgc cgccacccgc aagggcaaag
    15241 ccaacatgcc atccatacca ctgagcagcc aggccgcagg cgattggggc ggcgatagaa
    15301 ccgatattgc ccgcagcata cagcagcgaa aaaccgccat cacgtcgatg atcgttctcg
    15361 tcgtaaagct cgccaagcaa acagctgatg tttgatttga ataaaccgta gccacaaata
    15421 atgattgcca gcgccagata caggctaaag gttgaatttg tatcaatgcc cagcaccaca
    15481 tggccaaggg tcattaacag cgcgccggca atcactgcag tgcggttgcc gagcaggcgg
    15541 tcggcaagcc agccgccgag aataggggta acgtaaacca gagaagcata tgcgctgaac
    15601 aggctgatgg catggttatc atcaaaacca agctgatggg tgagatagag aatgagtaag
    15661 gcacgcatgc cgtaaaaact gaagtactcc cagatttgga tcgccacgat atagtatatc
    15721 gcgcgcggct gtgagggtgt tttcatgtgt tctccttatg agcaaaaaag ggaagtggca
    15781 agccacttcc cttgtacgag ctaattattt tttgctttct tctttcaata ccttaacggt
    15841 atagcggcca tcagcctgac ggtatgcacc gtgaatatcg gtttcaaagc ccggatagtg
    15901 agcgccgatt tcacacagca tctgcaggaa ctccagaacc ggacggcttt cttcggtgat
    15961 catttcaccc ggcattacca gaggaactcc cggcggatat ggaaggatca tattggcgtt
    16021 aatacgacct accatttcgt cgaggtaaac ttcttcggtc ataccgtgca gctctttctg
    16081 gaacgcagca tacggagtca ttaccatcgt cggcagcact tcaaatgcgc gatacatcag
    16141 atccggcaga ttgtggtgaa caatcagttt gtggatattc tgagccagtt cctgaatacg
    16201 catgttttca tagaattcag gatcttcacg atacagagac ggcagcatgt ttttcacacg
    16261 caggttcagg tcgaacgcac gtttgaagtc agtcagagca cgcagcaggc tcagtgcttt
    16321 ggtcttatcg ataccgatgc tgaacaggaa cagcaggtta tacggaccgg ttttctcaac
    16381 aacgatgcca tgttcgtcga ggtatttcgc cacgatgctg gccggaatac caaagtcgct
    16441 catggtgccg tctttttcca tccccggagt cagcagggtg actttgatcg ggtcaagata
    16501 catgtgctcg ttatcgatgt ttttgaagcc gtgccaggtg ctgtcagaac gcagcggcca
    16561 gcattcagtg gtatcgatat gatccggctg ccatacatca aagaaccagc catcagattc
    16621 cgttctcaga cgtttgatct ctttacggaa tttgatcgca cgttcaatgg aaccgttgat
    16681 cagacgctta ccagcattgc ctttcatcat cgccgctgcg gtttcagtgg acgccacgat
    16741 accgtagtgc ggagaagtgg tggtgtgcat catgtaggct tcgttaaagg tttcttcgtt
    16801 tacgtcacct ttaacgtgga tcatggaagc ctgagagaac gccgccagca gtttgtgagt
    16861 ggactgggtt tcgtaaatca ctttcccttc tacacggcca ccgctcatac cgcatttacc
    16921 ttcgtaaatc ggtgagaagt tggtgtaagg cacccacgcg gagtcaaagt ggatggattt
    16981 cacatccagt gttttcttga taaagtcggt gttgtacagc agaccatcat aggtagagtt
    17041 ggtaattaca gcatgtaccg gccaggttgc gtttggtgtt tctttcacgc gcttagcaat
    17101 ggtagcgtgc tggaattcac tctgtgggat accaccaaga ataccgtaag cgttacgggt
    17161 cgggcggaaa tagattggcg taacatcgct catcatcatc aggtgggtca gcgatttgtg
    17221 gcagttacgg tcaatcagaa tggtgctgcc tgctggagca gagtacatac caacaatttt
    17281 gttcgcagtg gaagtaccgt tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc
    17341 gatatactgt tctgcttctt tgtgtggacc actgtgatcc agcagagaac ccagttcaga
    17401 tactgaaatg gaaatatcag atttcatggt gttcggacca aagaaatcat agaacaggct
    17461 acctaccggg cttttctgga atgcagtacc gcccatgtga ccaggagtac agaaagtata
    17521 tttaccttca cgaacatatt taaacagtgc tttagtcagc ggaggcagaa tagtgttgat
    17581 atattcgtca gtggtctgct tgatcttatt agcaatatct tcagcagcac ccagcgcata
    17641 ttcaaagaag ctaatctgta aacgcaggtc attcaggctt acatcgagag tggaatacgt
    17701 attagcgaac gcgtacaacg gcaggttctc gttcattttg ctaatttctt cgcacagctc
    17761 gagattatat ttatcccagt caaaaataac gccgcacaga cgcgcattgt tttcgatcag
    17821 ttttaataag tcgtcacggt cgttcgggta aacaatctgg aagttcagac gttcaagcgc
    17881 gcgatgaagt tcacggatgg gttcttcttt aaaataaacc cccatgtgat tcaatattgc
    17941 aataacgttc atagtcatat ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa
    18001 tgaaaggagg agcctcggaa aatactttta attaatgtgc gttagacgcg gtgtggttat
    18061 ccattgagtg gctctggcgc tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc
    18121 tgacgatgaa ggtacctgcc agctcgaagg agcttgcgcc catcagcgcg atgaagcaga
    18181 acacgcaacc cagtacagag cagatcaggc tgacaaagtt gcggatgtta acgccttcaa
    18241 aacgaatcag gtcaacgcaa gagtagaagt acggcagcat agtcagcagt actgcgatac
    18301 cggtcagttc accgaacagg tcagatgctt taccaccggc agagttcatc agagtgatga
    18361 ggatcatcag ggcagtcatt ttcactgcag ccagcagcag accttttttc ggaataccgt
    18421 tgctgtcgac ttcaccataa actttcggga agttaccgtc gttagcggca cgtacacctg
    18481 cctggcctac caacatcatc caggagccca gagaagtcag gcacgcaaag gcggtgaatg
    18541 cagaaaccag cggcgcagcc cagttaccga ggatagttga agcactgatt gcaaacggag
    18601 caccggaagc cgccattaca gaagacggat acataccgga aagcacctga gtcgcagcga
    18661 tataaacaat acctgctaaa ccagtaccca gcatggttgc cagcggaacg gtacgtttcg
    18721 ggtttttaac cataccagta cttactgctg cggattcaac acccacgaag gcccacaggc
    18781 agagcagaat acttttaatg atcgcatgac catcagtggt atccgcagta ttccagttag
    18841 ctgcataagt tgccgcatca aaccaatgcc agccaacaat agcagtcatc accacaggaa
    18901 taagaaccag caccagacca atagtggtta aacggcttac ccaggtaccg ccgagcatat
    18961 ttacaaaggt aaatacccag acgatagcaa tacaggcgat acccgccgga acaggatcat
    19021 ttaatactgg gaagaaggtg gaaagataag atacagcggt aataccaatc gccaggttac
    19081 caatccagtt agcatggtaa taaagaacac ctgtctgaaa accaaatgca ggggaaattt
    19141 ctccggcata agcaattggg ccaccttgtt gcgggttttt tgttgccagt cgggcatata
    19201 catacgccag cgacattgca ccaataatag agataatcca accccagata gcaataccac
    19261 cgatacttgc taggttcgca ggtaataatg caataccgct ccccatcata ttaccggcaa
    19321 caacaccggt acaggcaaat agcccgatct tcttggcaga actcatgctc ttctcctaat
    19381 ttcatttttg aatttggagt ccgggtcatg atgtataact atttcctgac cagaccaaac
    19441 tggcgctaag aatactcacg aaaaaaggat taatcctaaa gattaggtga aataacacaa
    19501 aagtttctgt aagtgagaac ttgaggtttt ttattaacac atcaggatcg caagttgata
    19561 tcatgaaaag ataaacattc aatgtttaca atggattgcg ggatattctc tggttaaatt
    19621 tatgtaataa aaattatgcg gcaaataaat tgccgcaaca tattatacca acagaaacat
    19681 acaaaaattc aacaacaaat attcccgagc ataaatcaac ccgggagtta cttattctga
    19741 agcgagaaat ttgtcgagat aaggtacaac ataaggaaca gaagtctgga atataccatt
    19801 ttcaatccag taaagggtgt ttgcccctgg gcgtaaatta aaggcggtga gatatgcatc
    19861 agccgcttcc cggttcatcc ccttcatttc ataaaccttg ccaagcaaca cataatttag
    19921 ccaggacatt tcaagatcaa tgccagtatt tatcgcctgg taagactcat ctgttttacc
    19981 ttttaccaga gcactgaccg cttttatttg atatataatg gataggttat tcaattccgg
    20041 tagtgtaaca atgttgtcta tttctgtgtt cagtgctgct aattgttttt catctaaagg
    20101 gtgttgagaa tggcgcacga tatcaactaa tgctttttct gctctcgcgt aggtaaattc
    20161 tggggatgat tgaacaatct cacctaataa ttcactggca cggttcaatg atttatcatc
    20221 gccatgcagt aaataatcat gtgcctgata aaaattagtt aataacgcac cacgatgagg
    20281 caaaatttgc tggagcgtct cctgcattcg ttgtggccac ggttggttta atgcttttga
    20341 taaactctcc agtaaatcat tttgaatcgc cagctgatta ccgttagtga tgacataacg
    20401 tttatccagc atggttgaac catctgcatt gtctaccaat tttatcgaca taaagcattg
    20461 ttgagctcgg tattggcgct gattaacaaa ggcaatagat aatgttttac cggaactgct
    20521 cggttcatca atgttgtagt tgattttgtc atgcaccata aaggtggaga aggtgttaag
    20581 tgatgtcgcc accaaatcac ccacgcctat cgcgtaagag agctgatacg gggaactcca
    20641 gctgttacaa cttttattta ccatattaat gtcaatatcg cgtggattga gcaaaatacg
    20701 cgatttgctc ataggaagac gtgtatcaag agttgaaaac gctaccagtg ctacacagat
    20761 acctaacgac aacaggaaaa aaaaccatac ccaaaaggta gtgaatcgtt tgcttttaac
    20821 tggggattgt tcaggtggcg ttgcggtgtt ttgaatgtta agactgtggg agggagaatc
    20881 tgtggcagga accgcctctg gtataggggg aggcgaagat agcattattt cctctccctc
    20941 ttcttcgctg taccagataa ccggcaccat taatttatag ccgcgctttg gtacagtagc
    21001 gatatagaca ggactatctt catcattatc ttttaatgac ttacgtagtt ctgagatact
    21061 ctgcgtcaca acatgattgg tgacaatact tctcttccag acattatcga taagttcatc
    21121 cctgctaagt acttcgccac tgtgttgagc aaagaaaacc agaagatcga ttaatctcgg
    21181 ctcaagggta agttgacgcc cattgcggct aatttggttt atggacggag taacaagcca
    21241 ttcgccaacg cgaactacag gttgttgcat aatagaaact cattcgaaaa gggaatgatg
    21301 caatgataat tgccacaacc tatttttacc atctatagat gggtttattt acatattatt
    21361 ggtgaatgta agacgttatt tttaccagcc ataaacttct tgattacata gtattacgaa
    21421 aggattttac tgagaaccag aagtaatatt ccttaccatc aaaattcatc atctttgcca
    21481 aagaaaaatg ttcagaaaat aatccatgga aaattgtccg gagcgcttac tattttaatg
    21541 gattgttagt ctttgcatga gcaagcggac tgatacattt ctctttgttc tcattcagaa
    21601 aatctcatca gtcggcgttc agaccggtgt tgtgctttat ggatgcgtta cgctcctgat
    21661 gacgtcattt gacgttcaac aacatcacgg ggccgcacga catttcacgt cagttagtgc
    21721 tatcgctcag gaacaaattt tcccgaattg ggatatgccc gcaaattgct ggtgatgtgg
    21781 ggagaatctg gttgagttcg ggagaattga tttggaggca gaacgcttaa attgtggcgt
    21841 ccagaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
    21901 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
    21961 gcaacggggc gcattaaacc gtaatctgca catctcgtca acctaatttc agtaaaagcg
    22021 attcaactgc ttaagattgc ggcaaatcgc ttcttttctg tgtttctgtc aggtcaatgc
    22081 accaccctgg cggcagcggg cgaagcgcag gatatcttcc gccagccgat gtgcagtgtc
    22141 gacatccgcc tggctacgat tcaccagcat tcggcttaaa cagccttcca gcaccagttc
    22201 catctgcttt gctaccatcg ccggatcgtc aacttccagc gtggttaaca gttcgtgggt
    22261 gaaatcgtag gccgcgcttt tttgctgatc ggccagttga tgaatagggt ggccaggatc
    22321 gggataaaac gtacaggcag cgataaatag acagcccgga tagcggttgt ttttaacgca
    22381 ctccgataac gcctgataac gtgccagcaa cttttgttcg gcggtttgcg tttcgtccag
    22441 catcagctga cgacgccaga catctatctg ttggctaaga taacgcagcg catcgtagag
    22501 gattgcctct ttgtctggcc agaagcggcg tagctcgtcc agtggataat ccacacgttc
    22561 agcaaccatc tccagcgtgg tgttggcaat cccttgtaat tctaataatt tcagggcttc
    22621 tcccagtaca tcttcacgtt gcacgctatt ttcctccgtc tttcccactg caagtgtcgt
    22681 tcacggttgg cgatcgcgca aatgtgcgct gaaggtttca gcatccataa agcccgtgac
    22741 gcgtgcttgt ggatgctcct ggccttgtcc gtcaaaaaag agaattgtcg gtaggccaag
    22801 gacattaaga tgctttaaca gcgccacatc ttgtgcgtcg ttggccgtga cgttggcctg
    22861 aagtaagacc gtgtctgcta acgctttttg cacctgcggg tcgctgaagg tgtatttctc
    22921 aaactcttta caggcgacgc accagtcggc ataaagatct aacatcaccg gtttgccttt
    22981 ggcttcaacg agcgcctgat ttaactcatc taccgttttg atttgtgtaa agttgagatg
    23041 cgtctgagtt tgcgcggtat gcgtcgcacc aaatgcccaa tcctgaagtg ggcgcacgct
    23101 aaccaatgcc gctgccagca ggattatttg caccacgcgc atccagccgc gtttagcctg
    23161 taggctggtg ataaaggccc agccaaagaa tgcgacaccc agcgccgacc acaagcgtaa
    23221 tccccataca tcaccaatca ctcgctccag caggaagacc ggcagtgcga ggatcacaaa
    23281 accaaacgcg gttttgactt gttccatcca cgggccgctt ttcggcagca agcggttacc
    23341 aaagacggta attagcatca gcggcaggcc catgcccaac gcatagagat aaagcgtgcc
    23401 gccgcccagc cacatgttcc cgctttgggc gatatacagc agaatcgcgc taagcggtgc
    23461 ggtggtgcat ggtgaacaga tcagtccggc aatcgccccc ataacaaaca caccgccagg
    23521 tgagccgccc tgttggcgat tgctcatcaa cgtgagacgt gtttgcagcg aagaggggag
    23581 ttgtagagta aacaagccaa acattgacat cgccagcaag gtaaagacga tggcgaggcc
    23641 aatgagcacg tatgggtgct gtagcgccgc ctggaactgt aaccctgcgg cggcaactac
    23701 cagacccagc gccgtgtaag tcagtgccat cccctgcaca taaataaagg tcagcaacaa
    23761 tgctctggca gtggagagcc gctgtttacc acccagcacg atgccagaaa tcagtgggta
    23821 cattggcagc acgcatggcg taaaggcgat accaataccg atcaacaacg cccagagcgc
    23881 ggaaaagggc aattgcgcgg tgtgctgctc ttgctgcgaa acagacacag gctgtgacgc
    23941 tgcgttgttg gcgaccactt cgcttaacgg aacggttttg gtttctggcg gataacagaa
    24001 accggcatca gcgcagccct ggtaggtgac agttaacgtc gctcccgcac tcgcctggtt
    24061 gatggttacg ggaagcgtca gccgatcgcg gtaaatctcg cttttgccgt aaaactcatc
    24121 ttcatgccag acgccttgcg gcagctgcac gtcggcaatt ttcgcgtgtt ccggcgtaat
    24181 gcggatctgt ttacggtaga ggtagtaacc gtctttgatc tgccaggtca gattaaggtc
    24241 atgttggttt tgctgaaaat caaaagtaaa ggcttgatcc gcggggacaa attgtgaacg
    24301 tcccggcgcg tcgaataatc cggcaaaaac ggaagtgctg caaagtagca ggatcagcgt
    24361 aaagatgcgt tgagccatga gaggtaatct gtgtctccgt gtgtaacagg taaaaccaga
    24421 agttccgggg tttgatatgg atgatgagac ttcaggcatt ccagcagtgc ctgctggtga
    24481 gatacggtag tttttaaaat catctgcact tcgtattctt gctccagctt accttcccag
    24541 taatagagag aggtagcgcc ggggatcaag gtcgcgcagg ccgccagttt ttccgccagc
    24601 actttggcgg ctaaatcctg ggctgtcgct tcatccggtg cggtacatag caccacgaca
    24661 gacgcggtat tcgaactttt ttcatcaagc ataaacacct cgcaagaaca gatggaaccg
    24721 caagagagag gtcactatac aacggacggg ggaaggatgt tagtcggcgg gaaagaaagc
    24781 ggggcgtgaa cgccccgcga ttgatcatta cagcatgaag ctacccagca cgaagccgaa
    24841 gcaaacggcc agggcaacac ccagagtacc cgggatgaag aacggatggt tgaagacgaa
    24901 tttaccgata cgggtagtac ccgtgtcatc catctgtacc gcagcaacca gcgtcgggta
    24961 ggtcggcaga atgaacagac cagacaccgc agcgaaagaa gcaacagcgg tcagcggtga
    25021 aacgttcagt gccagagcca tcggcatcag tgcttttgcg gttgcagcct gagagtacag
    25081 cagagcagaa gcaaagaaga agatgacggc cagcagccac ggatgaccct gaatcacttc
    25141 accagcggta tctttgatcc agtcgatgtt gttggaaacg aaagtatcgc ccagccacgc
    25201 aacacccagg atacaaatac aggcgctcat acctgctttg aaggtgctgg agttgaggat
    25261 gttgtcggta tcgactttac agataacggt ggtcagagtt gcaacgctga gcatgatgat
    25321 caggattgcg ttggtggtgt tcatcagcgg tttttcaacc agacccatgc ttgggctgtt
    25381 gatgattgca tagataacca cgccaactac gcccagcagg aacagccaga cggacgtttt
    25441 tgcaccggat ttgatttcaa tctgcttttc accgcgcagt tcaaccaggc cctcttccag
    25501 acgcttgcga taaatcggat cgtcagagag tttggagttg aacagcatag tgaccaggaa
    25561 ggacatcacc agaaccgcca gcagggtgga cgggatgacc acggagagca gatggaggta
    25621 gctgatgcca tgaccttcca tcacggaaga catgtaaacc actgccgctg agattggcga
    25681 tgcggtgatc gcaatctgcg cggataccac tgcagtagac agcggacggc aaggtttaac
    25741 gccttgttcc ttcgcaactt cagcgataac tggcagtgtc gccagagaga tgttgccagt
    25801 accagcaaag atagtcagga aataggtcac gatcggtgcg aggatcgtga tgtatttcgg
    25861 gttacggcgc agcagctttt ctgtctgatg aaccagatag tccagaccgc cagcaacctg
    25921 catggcagaa atagcggcga taaccgccat gataatggag atgacatcga acgggatgtt
    25981 accgggttta acgccaatag cggcaagaac cagcaccccc aatccgcctg caaaaccaat
    26041 acctattccc cccaatctgg cgcccaagaa gatcgccagc aaaactatga tgagttctac
    26101 aactagcata ttagccttcc ttgtttttta acaagttgat attagattgt tatttttaag
    26161 ttactgctca caagaaaaaa ggcacgtcat ctgacgtgcc ttttttattt gtactaccct
    26221 gtacgattac tgttcgcttt catcagtata gcgttttgct ttgtaagccg ggtgcatcag
    26281 attctgtacg gagaaaatat cgtcaagttc cgcttcagtc aacagaccgc gttccagaac
    26341 gacttcacgt acactcttac cggtttcggc acagatttta cccacgatgt caccgttgtg
    26401 gtgaccgatg aacgggttca ggtaagtaac gataccgata gagttgtaaa cgtaaccttc
    26461 gcacacttct ttgttagcag tgatgccgtt aatgcatttt tccagcaggt tgtagcaagc
    26521 gttggtcaga atgtgaacgg attcgaacat ggcctggcca atgaccggct ccataacgtt
    26581 caactgcagc tgacctgctt ctgctgccat ggtaacagtg gtgtcgttac cgatgacttt
    26641 gaagcatacc tggttaacca cttccggaac aaccgggttt actttagctg gcatgatgga
    26701 agagcccgcc tgcagttccg gcaggttgat ctcgttcagg ccggcacgtg ggcctgaaga
    26761 gagcaagcgc aggtcgttac agattttgga catcttcaca gccaggcgtt tcagcgcgcc
    26821 gtgaaccata acataagcgc cgcagtcaga ggtcgcttcg atcaggtctt cagccggtac
    26881 gcatgggaag ccagtgactt cagccagttt tttcactgcc agcggagagt actctttcgg
    26941 cgtgttcaga ccagtaccga ttgctgttgc gccaaggtta acttccagca gcagttcagc
    27001 ggtacgttgg atatttttca cttcttcttt cagcaggatg ctgaaagcgc ggaattcctg
    27061 accgagggtc atcggtactg cgtcctgcag ctgggtacga cccattttca ggatgtcctg
    27121 gaattcgaca gctttacgtt caaagccttc acgcagttgg ttaatcgcat ctaccagctt
    27181 aatcagggaa gagtaaactg cgatacggaa accggtcggg taggcgtcgt tagtggactg
    27241 acatttgtta acatggtcgt tcgggttcag gtactgatat tcacctttct ggtgacccat
    27301 cagttccaga ccgatattgg ccagcacttc gttggtgttc atgtttacgg aagtacctgc
    27361 gccgccctgg tagacgtcta ccgggaactg atccatgcat tttccgttgt tcaggacttc
    27421 atcacatgcg gcaatgatgg cattcgctac acttttagga atggtttgca gctctttgtt
    27481 tgccatagct gcggcttttt taaccattac cataccgcga acaaattcag gaatatcact
    27541 gattttgttg ttgctgatat agaagttttc aatcgctctc agagtgtgaa caccatagta
    27601 ggcatcagct ggaacttccc tggtacccaa cagatcttct tcgatacgaa tgttgtttga
    27661 catgtgaacc ttctttttca agctgccaat gatttgcttt aaacacacag aatatatgtg
    27721 gtttcgaatg tttttcgacc gacgattatc ccctgcatcg accgaatacc cgagatcata
    27781 tgctgcttga ggatttctac cgtaatctgg atcactttaa gtgtcggttt ttacccctta
    27841 attattaatt tgtgaaatag atcaccgctt tgggattact accaaaaata gttgcgcaaa
    27901 catcttgaaa ttttgctaat gaccacaata taagctgaac gcgattcgca acccattcag
    27961 gtagccgggt tttaccggct gctattacag gagaaacctt tgcgctggtt accttttatt
    28021 gccattttcc tttatgtcta tattgagatt tcaatcttta ttcaggttgc ccatgtattg
    28081 ggggtattgc tgaccctcgt gctggttata ttcacgtcag ttatcgggat gtcactggta
    28141 cgtaaccagg gctttaagaa tttcgtgctg atgcagcaaa aaatggcggc gggtgaaaac
    28201 ccagcggcgg agatgattaa aagtgtttcg ctgatcattg ccggtttgct gcttttatta
    28261 ccgggctttt tcaccgactt cctcggtctt ctgcttttat taccgccggt gcaaaagcat
    28321 ctgacagtga agttgatgcc gcatttgcgc ttttctcgta tgccaggcgg cggttttagc
    28381 gccgggaccg gtggcggtaa tacttttgat ggtgagtacc agcgaaagga tgatgagcgc
    28441 gaccgccttg atcataaaga cgatcgccag gattaatgtc gaaacgccgg attatgtggt
    28501 tatgccattt tccggcgttt ttcgttttgg cagcaacagc cataaccccg ccagcatgat
    28561 cagcgcatag agacttttcc agccgaccat tgccagtaac agaacgcata acagcccgcc
    28621 aaccaccgcc agtagtcgat aacgtccttg caataattta cagcctgcca gcatgcataa
    28681 cagataaatc ataataaaga tgccattggc ataaataata agagcgtcca gattgatctc
    28741 taaagcatga atcaccaacg tgctcaccac acagcagccg agcaccgcat tgagggcatt
    28801 attcgggata tggcgagaag agaggcgtgc caggtagtgg tcaggattat gttgcgcctg
    28861 cgaccagacc aggcgggcga agctctgtat ataaatgttg agactggcaa agcaggccag
    28921 atagccaatc acgcaggcaa tccataacgc tcctacaccg aacaactgca ctacaatttt
    28981 tggaagcgat gctgccgccg ccattttttc accataggcg tcgaagtgta agacgactac
    29041 cgtacagccc cagtagacta atcctgccag cagcagacca atcatcaatg cgcgaggaaa
    29101 atcacgctct ggatttttaa attccgaggc aagatgggca aatgcttcca gaccgacaaa
    29161 acaccagaac atcactgata acgcagcaaa taacccggta agttcgatat taccaggtgc
    29221 cgggaagggg atattcgcag gtttgatatc gcccgcccac cagatagcga caatcagcgc
    29281 gacgataagt ccggcaataa cggtttgtag attagcactg gaactggcac cgcgagtacc
    29341 gatataccac accagcgcca gcgtaccgag ttctgccaac aacagttgcc agctatgcca
    29401 gccaaacatt gcctggccga acccggcggc aatttgtagc gcggcaggca aacccacggg
    29461 aatgaccgat aaaaacaacc agccggtgac tcgctcaagt cgcgaaccaa acgccatacc
    29521 gacgaagtgc gcgacaccgc ctgcgctggg atagtggcga cccagaatcg caaacacaat
    29581 cgcaatcggg aacactaaga taatcaaaac gggccacgcc cacaggctgt tattgcccgc
    29641 taccagcgca gctaacgcag gaacggcaaa cacgccagtg cctaataatg acgtcgatag
    29701 cagtccaatg ccctgggcca gccccagttc ttgtttgagt ccactcatgg gttgatatcc
    29761 gattgcgccc aaattttggg caactgcgta gattttcgat ggtagcacaa tcagattcgc
    29821 ttatgacggc gatgaagaaa ttgcgatgaa atgtgaggtg aatcagggtt ttcacccgat
    29881 tttgtgctga tcagaatttt ttttcttttt cccccttgaa ggggcgaatc ctcatcccca
    29941 tttctctggt caccagccgg gaaaccacgt aagctccggc gtcacccata acagatacgg
    30001 actttctcaa aggagagtta tcaatgaata ttcgtccatt gcatgatcgc gtgatcgtca
    30061 agcgtaaaga agttgaaact aaatctgctg gcggcatcgt tctgaccggc tctgcagcgg
    30121 ctaaatccac ccgcggcgaa gtgctggctg tcggcaatgg ccgtatcctt gaaaatggcg
    30181 aagtgaagcc gctggatgtg aaagttggcg acatcgttat tttcaacgat ggctacggtg
    30241 tgaaatctga gaagatcgac aatgaagaag tgttgatcat gtccgaaagc gacattctgg
    30301 caattgttga agcgtaatcc tcgcacgaca ctgaacatac gaatttaagg aataaagata
    30361 atggcagcta aagacgtaaa attcggtaac gacgctcgtg tgaaaatgct gcgcggcgta
    30421 aacgtactgg cagatgcagt gaaagttacc ctcggtccga aaggccgtaa cgtagttctg
    30481 gataaatctt tcggtgcacc gaccatcacc aaagatggtg tttccgttgc tcgtgaaatc
    30541 gaactggaag acaagttcga aaatatgggt gcgcagatgg tgaaagaagt tgcctctaaa
    30601 gcgaacgacg ctgcaggcga cggtaccacc actgcaactg tactggctca ggctatcatc
    30661 actgagggtc tgaaagctgt tgctgcgggc atgaacccga tggacctgaa acgtggtatc
    30721 gacaaagccg ttaccgctgc agttgaagaa ctgaaagcgc tgtccgtacc gtgctctgac
    30781 tctaaagcga ttgctcaggt tggtaccatc tccgctaact ccgacgaaac cgtaggtaaa
    30841 ctgatcgctg aagcgatgga caaagtcggt aaagaaggcg ttatcaccgt tgaagacggt
    30901 accggtctgc aggacgaact ggacgtggtt gaaggtatgc agttcgaccg tggctacctg
    30961 tctccttact tcatcaacaa gccggaaact ggcgcagtag aactggaaag cccgttcatc
    31021 ctgctggctg acaagaaaat ctccaacatc cgcgaaatgc tgccggttct ggaagccgtt
    31081 gccaaagcag gcaaaccgct gctgatcatc gctgaagatg tagaaggcga agcgctggca
    31141 actctggttg ttaacaccat gcgtggcatc gtgaaagttg ctgcagttaa agctccgggc
    31201 ttcggcgatc gtcgtaaagc tatgctgcag gatatcgcaa ccctgactgg cggtaccgtg
    31261 atctctgaag agatcggtat ggaactggaa aaagcaaccc tggaagacct gggtcaggct
    31321 aaacgtgttg tgatcaacaa agacaccacc actatcatcg atggcgtggg tgaagaagct
    31381 gcaatccagg gccgtgttgc tcagatccgt cagcagattg aagaagcaac ttctgactac
    31441 gaccgtgaaa aactgcagga acgcgtagcg aaactggcag gcggcgttgc agttatcaaa
    31501 gtaggtgctg ctaccgaagt tgaaatgaaa gagaaaaaag cacgcgttga agacgccctg
    31561 cacgcgaccc gtgctgcggt agaagaaggc gtggttgctg gtggtggtgt tgcgctgatc
    31621 cgcgtagcgt ctaaactggc tgacctgcgt ggtcagaacg aagaccagaa cgtgggtatc
    31681 aaagttgcac tgcgtgcaat ggaagctccg ctgcgtcaga tcgtcctgaa ctgcggcgaa
    31741 gaaccgtctg ttgttgctaa caccgttaaa ggcggcgacg gcaactacgg ttacaacgca
    31801 gcaaccgaag aatacggcaa catgatcgac atgggtatcc tggacccaac caaagtaacc
    31861 cgttctgctc tgcagtacgc ggcttctgtg gctggcttga tgatcaccac cgagtgcatg
    31921 gttaccgacc tgccgaaaaa tgatgcagct gacttaggcg ctgctggcgg tatgggcggc
    31981 atgggtggca tgggcggcat gatgtaattg ccctgcacct cgcagaaata aacaaacccc
    32041 cgggcagaaa tgtctggggg tttttctttt ggtcatcttt ctagtataag attcagacac
    32101 ggacgacgcg agtggcgtcc agctcattga ttatggggaa taacatgcac gtaaaatact
    32161 tagcagggat tgtcggtgcc gcgctactga tggcgggttg tagctccagc aacgaattga
    32221 gtgctgccgg tcagagtgta cgcattgtgg acgagcagcc aggcgcagag tgccagctga
    32281 ttggtactgc gacaggtaag caaagcaact ggctttccgg gcaacacgga gaagagggcg
    32341 gttctatgcg cggcgcagca aacgatctgc gcaaccaggc ggctgcaatg ggcggtaacg
    32401 tgatttatgg catcagcagc ccgtcgcagg gaatgttgtc cagttttgtc ccgacggata
    32461 gccagattat cggtcaggtt tataagtgcc cgaactgatg tgatggtgac aaacgtataa
    32521 ttgccagagg cgcttcgctt atcaggccta cataaggcat tgcaactgat tgaatgcttg
    32581 agaatttgta ggccggataa ggcgtttacg ccgcatccgg catgaccaac gatcacgtta
    32641 tcagtcattt caggctggat ggctccagcc tcgcattagc aaagatgaga ttatttcgcc
    32701 tgtggtgcag ttttgctggt ggattgaaca cgtaagtgat gcagcgcgcg taagcattga
    32761 tcctgggtta gcggttgggc gttattcgcc gctacggtgc gaacatagac ctgacatggc
    32821 acacccgcga gttttttcag tcgtgggctg aaatcaagca ttcggcgagt gatcgcctct
    32881 atttctttac ccggcgttgc ttcacgcgtt ttcaccactg cgccgctaaa ttgttctttg
    32941 ccactttcat ctttaaagcg gtaaagcacc gccagataat ggtcgaacaa gttatgtttc
    33001 cattccggtt gggaataggt gtcatattcc agattgccat tttcctggca gtcaacgtca
    33061 tataacggca ggaaatctaa cagtgaggta atacgtaatg ccagttcacg catttccgca
    33121 ttattaattg catgaatgat tgctcgcgcc agcatttcaa tctgcaactc attgacgact
    33181 aactcacatt tgctaccgtc atgtagcgtt aatacgaagg taagattttc gccttgatta
    33241 tcggtaagtt ccagcgtgtt aacccgacga ttaatatcgg catttttaag ctcatcaacc
    33301 agaatttctg ggatgttttc tgccattttc ttaatgactt tatcgcgagc ttgctcatac
    33361 tgaaggcgtg cagcagcatc cagtttatgc ttttgatgca gacgactttc cagcgcaatc
    33421 agcaggtcgc gcagttccat gacagacata aagaacaacg attctttatt gcgaggttct
    33481 ttaattttca gcgccagtgc aatgaaatta ttactcttgc gaataacacc ggtattaaca
    33541 cccttgatgc ttatggccat gtaggttctc cctaaccatt tctcaattaa ataattaatt
    33601 ttaatttata agccagataa atgggcttgg tagtaatagt tgttaaaata acataaatag
    33661 ccgtgctcac tctatataaa cagaaaagaa aggttaatta ttggtgttag ctatataaaa
    33721 agtaacaaca gcaatgcata tgaataatta aaggttatgc aacgtgcaaa gattaaattt
    33781 ctcagtttaa tgacaggttg ctgtacttgc gtttgttgct cttgccggat gcggcgtaaa
    33841 cgccttatcc ggcctacagt tcattgcaaa tccattcaat ttcaatgcac gatgtaggcc
    33901 tgataagcgt agcgcatcag gcaattttaa tgtttaactt ccctgtttaa tcagttactg
    33961 ctggcgtagc tggagatcca gcggcgtttt gctgggttcg ccgccaattt ctcgcgccag
    34021 tttcggcacc agatatcccg acaccaatgt cagcaactca cgcataatct gccgtgcttc
    34081 gtcatcactc accataaaat gcgccgcgcc ctgtacttta tcgagcacat gcaggtaata
    34141 cggcatcacg ccggcatcga acaacgcatt actcaggttt gccagcgttt gtgcgttatc
    34201 gttcacatca cgtaacagaa cgctctggtt cagcaaagta acgcctaccc ggcgcaactt
    34261 agccatcgcc tgacggaatg tttcatctat ctcattggca tggttgatgt gattcaccag
    34321 caagatttgc agcgtagaat gtgaaaagcg ttcaaccagc gcatctgtga tgcgtgccgg
    34381 gatcacaatc ggcagacggc tgtgaatccg cagacgtttg atatgcggga tggcttccag
    34441 ttgtgtgagc aaccagtcca gctcgtgatc tttcgccatc agcggatcgc cgccagagaa
    34501 aatcatctcg tccagttccg gatgcgcagc aacatactca agtgcagtct gccagttacg
    34561 cttgttgccc tgattttcgg cataggggaa gtggcgacgg aagcaatagc ggcaatttac
    34621 cgcgcagccg cctttgacca gcaaaagcgc ccggttgtgg tatttatgca acaaaccagg
    34681 cactacgctg tgctgttctt ccagtgggtc ggtggagaat ccggacgcga cgacaaactc
    34741 atcttgcgag gtaagtacct gacgcaaaag aggatcgtcc ggattgcctt tctccatgcg
    34801 atcgataaat gagcggggca cacgcagggc aaacagcttt ttggcgctgc gtccggccaa
    34861 cagtttttcg tccgcgtcta tattcaaaag acgcagaagt tcatcaggat cggtcacaac
    34921 atcggcaagt tgcgttaacc aatcttctct ggatggggta tttagggtta caatatgcgc
    34981 cattttgtgg cttagctacc aattaacaaa tttcagaggg ccttatggca acgtactata
    35041 gcaacgattt tcgtgctggt cttaaaatca tgttagacgg cgaaccttac gcggttgaag
    35101 cgagtgaatt cgtaaaaccg ggtaaaggcc aggcatttgc tcgcgttaaa ctgcgtcgtc
    35161 tgctgaccgg tactcgcgta gaaaaaacct tcaaatctac tgattccgct gaaggcgctg
    35221 atgttgtcga tatgaacctg acttacctgt acaacgacgg tgagttctgg cacttcatga
    35281 acaacgaaac tttcgagcag ctgtctgctg atgcaaaagc aattggcgat aacgctaaat
    35341 ggctgctgga tcaggcagag tgtatcgtaa ctctgtggaa tggtcagccg atctccgtta
    35401 ctccgccgaa cttcgttgaa ctggaaatcg ttgataccga tccgggcctg aaaggtgata
    35461 ccgcaggtac tggcggcaaa ccggctaccc tgtctactgg cgctgtggtt aaggttccgc
    35521 tgttcgtaca aatcggcgaa gtcatcaaag tggatacccg ctctggtgaa tacgtctctc
    35581 gcgtgaagta atgcggttgt ggtgcggcct gcaggctgca ccatcactta ttcaggtcag
    35641 agatgatgaa acgccttatc gttcttgttt tgcttgccag cacgctgctc acgggctgta
    35701 acaccgctcg cggtttcggc gaagacatca aacatctcgg caactccatc tctcgcgctg
    35761 ccagctaatt tttcttctct tccgaaaaat catcagattt ccgtcatttt tggcgatgtt
    35821 gtctattatt aatttgctat aggcaaacat aaataacatt acctaaaagg aagacgttat
    35881 ggtgaagaag acaattgcag cgatcttttc tgttctggtg ctttcaacag tattaactgc
    35941 ctgcaacacc acgcgtggcg ttggtgaaga catttctgat ggcggtaacg cgatttctgg
    36001 cgcagcaacg aaagcgcagc aatagcaata agcaataacg gtacgacagc tgtgtcgtgc
    36061 cgtttgtttt ttctgcgata gtcactaagg taatagttga aattcccctg ccacctggca
    36121 aaatatccgt tcaaccatca gctttgcagg acgacctgca aacgcgtctt ttcaccgggg
    36181 acggccccaa ttctccggag cctgatatgt cctggattat cttagttatt gctggtctgc
    36241 tggaagtggt atgggccgtt ggcctgaaat atacccacgg ctttagtcgt ttgacgccga
    36301 gtgttattac cgtgacggcg atgattgtca gtatggcgct acttgcctgg gcgatgaaat
    36361 cgttaccagt agggacggct tatgccgtgt ggacgggtat tggcgcagtc ggcgcggcta
    36421 tcaccggcat tgtactgctc ggtgagtccg ctaacccgat gcgcctggcg agtctggcgt
    36481 taatcgtatt ggggattatt ggtctgaaac tcagcactca ctaactaccg ggctgctgta
    36541 cccaaataaa cttactgaca tcaaaccctt cccgggtcgc gactgccagc atctcctgtt
    36601 tcacttcgtc agaaatggtt ggcgtgcggg agagtatcca caggtagtcg cggtccgggc
    36661 cgcaaaccag cgcatggcgg tattcccgat caagtgcaat gacgttataa ccgccataga
    36721 aaggaccaaa gaatgacact ttcagcgcag cgcgtgttgg tgcgccggta aagtacgctt
    36781 tcccttcact ctgctgccac atttctctgt cagggttata gcctttattg atgacattca
    36841 ggccgccgtc atcacgcaga ctgtatgttg cggtgacttt ttccagtcca cgttcaaaac
    36901 ggtgatcaaa acgggcaatc tcataccagg taccgagata gcgtttagcg tcgaaattat
    36961 tcactactgt cacgccacgc ggcggcgtag gagaactgca ggcaacgacc agaaatgcag
    37021 ctgtcgctgc ggcaacgaga gggagcaggc gcataaatgt ttccttactg gtttttttct
    37081 aagtgtagat gacagcaagg aaaagcgcag aaaaggtccg aaaattcgga cccgatgaaa
    37141 ttttactgta gagcgttgag aatctgccag gcggcggtga ctctcgctgg attgggatag
    37201 tttttgttag ccagcatcac tatacccagc tctttttctg gaataaacgc gacatagcta
    37261 ccaaatccgc cggttgcccc tgttttatgt acccatgatg cgcgtactgc aggagttggg
    37321 ggcgtaatcg cttttacggg gcgtgctgcc agtgcaattt tattgccact gccgttaatg
    37381 atgctgtcag gatttaccgg ccagtccagc atttcccagc ccaggccctg atacatatcg
    37441 ccggtttgcc agtagcgaga ttgtgccagt tgtatccctt gctgaagtgt tttgtcgttg
    37501 atatcacggg gattcatatt gcttcgtacc cagcaggcca tatcttcaat ggtcgacttc
    37561 acaccataag tttcagcatc taacgcccct ggcgaaacat gaactgcctt gccttcgcga
    37621 tatccccagg cgtaattctt ttcttctgcg ggcggtacat taatccacgt atggttgagt
    37681 ttgagtggct ggaagacacg tgtttgcatc gcctgctcaa aactcaaacc agacggcttc
    37741 acagccagtg cgccgaacaa accgatactg gagttggcat acagacgttg tgttcctgga
    37801 gcccatgcag gctgccagtt ttgatagaag cgcagcaagt cgcttgagga tttcacctca
    37861 tccggcacct gcaatggcag gccgccagcg gtgtaggttg cgagatgtaa tagtgtgatc
    37921 ccgttccact gtttagcggt aagttcaggc cagtattttg ttgcgggatc gcttaacttg
    37981 atttcccctc gagcaatagc gtcgccacca agcacgcccg taaatgtttt gctgaccgaa
    38041 cctaactcaa acaacgtttg ctgtgtgacg ggctgctttt tggcgatgtc cgcatagccc
    38101 caggtaaagt aataaggttt accctgataa attaccgcca ccgccatacc ggggatcttt
    38161 tgttgctcta taagcggggt aattgtgcga tgcacaatat cgttgatttt ttggggggca
    38221 gcaaatgtgg agcaagaggc ggtaattaat aaggcgcaga gcgtcgtttt gaacataggg
    38281 tctggtttcc atacaaaacg gcccgccata ggcgggccgg atttacattg gcgatgcgtt
    38341 agattgtaac gacaccaatc agcgtgacaa ctgtcaggat agcagccaga ccgtagaaaa
    38401 cccatttgcc cgcaggtacg tggattttca gatcgtgcat cgcgtggtgc atacggtgta
    38461 aaccacacca cagcggcaga acgatcatca ggaacaggaa tacgcgacca atgaagctct
    38521 gcgcgaacgc cagaacgcgc tcgtagctca gtgcatcacc cggaaacagc cccagcggca
    38581 gcagaatacc caccagcagg atcatcaccg gcgcaataat tgcgctccac ataccaccgg
    38641 ccccgaagag gccccagaat accggttcgt cagaacgctt tggatttgga ttgatcatct
    38701 caggctcctt accagtacag ggcaacaaac aggattacga tggtggcaac cacagttacc
    38761 gcccagagac ttttgataat tggctctgga cccatttttt cgtcttttac aatgatattg
    38821 gccgctttcg gtgccagttc aaaccaggtt ttggtgtgca gcagagctgc cgtcagagtg
    38881 atcaggttaa tgatcacaat aaccgggttt tgtaaaaagt cgacgaatcc cgcccaggct
    38941 tccgggccat ttttcagggc aaacagcccg aaaatcagtt caatgctgaa ccacacagcc
    39001 ggaaccgccg tgccttcgcg cagcatgtaa aagcgataaa acggcaattt tttccaccag
    39061 gtggacgtca ttggccgtac atacggttta cgtttagtcg tcatgttgca ctccttagcg
    39121 tggtttcagg gtcgcgataa gaaagtcttt cgaactttct actttgccct gctgaatggc
    39181 cgcagccgga tcgacgtgtt tcgggcagac ttcggagcaa tagcccacga aagtacagct
    39241 ccatacgccg ttctggctgt tcaactgcgc catacgctcc ttcttaccgt ggtcgcggct
    39301 atcttcgtta taacgatgcg ccagcgtaat ggcagccgga ccgatgaact ctgggttcag
    39361 gccaaactgc gggcacgcgg cgtagcacag accacagttg atgcaaccgg agaactggtg
    39421 atacttcgcc atctgcgccg gggtctggat gttagtaccc tgatccgcgg tgcgggagtt
    39481 gccgatgatg tacggtttga tcgcttccag actttcgatg aagtgggtca tatcgaccac
    39541 cagatcgcgt tcaatcggga agttagctaa cgcttcaacc ttcataccgt cggtgtaatc
    39601 acgcaggaag gttttacatg ccagttttgg cacgttgtta accatcatgc cgcaggaacc
    39661 acaaatcgcc atacggcagg accagcggta gctcaggtcc ggtgccaggt tgtctttgat
    39721 gtagcccagc gcatccagta atgaggtagt tgcgtcataa ggcacttcat agaatgcgct
    39781 atgcggtgcg gtatcgactt ccgggttata gcgcaccacc tcaattttca ggtttttcat
    39841 ctcagccatt cgccttctcc ttcttattgg ctgcttccgc cttatcggct gcatccgctt
    39901 cgccaccgta aacgcgttta gctggcggca gcgtagtaat cttcacgtcg ctgtactcca
    39961 ggcgagtcgt gccatcagca tcgcggaagg cgagggtgtg tttgaggaag ttgacgtcgt
    40021 cacgctcggt gcaaccttcg tccagacgct ggtgtgcgcc gcgggactct ttacgtgcca
    40081 ttgcggagtg cgccatacat tcagcaacgt tcagaccgtg gcccagttca atggtgtaga
    40141 gcaggtcggt gttgaacacg ctggaagtgt cggtgatgcg cacgcgcttg aagcgttcct
    40201 gcagctctgc cagcttgtcg atggttttct gcatcagttc cggcgtacgg tagataccgc
    40261 aaccttcttc cattgccagg cccatttcgt cgcggatctt cgcccagttt tcgccgccat
    40321 cctggttaac cagatctttc agacgttgtt caacgccagc tgcctgcgct tcaattgccg
    40381 cttcgttgcc attaccggca gttgctgcac gctctgtcgc ttgttcaccg gccagacggc
    40441 cgaagaccac cagttccgcc agggagttgg agcccagacg gtttgcaccg tgcagaccaa
    40501 cagaggaaca ttcacccacg gcgaacagac ctttaatgcg ggtttcacag ttctgatcgg
    40561 tttcgatacc gcccatggtg tagtgtgcgg tcggacgtac cggaatcggt tctttaaccg
    40621 gatcgacgcc aacgtacgct ttcgccagtt cgcagatgaa cggcagacgt tcatgcagtt
    40681 ttttctcgcc gaggtgacgc aggtcgagat aaaccacatc accacgcggc gtggagatgg
    40741 tgttgccttt acgccattcg tgccagaagg cctgagaaac tttgtcgcgt ggacccagtt
    40801 ccatatattt gtttttcggc tcgcccagcg gagtttccgg gcccatgccg taatcttgca
    40861 gataacggta gccatttttg ttgaccagaa taccgccttc accacggcag ccttcggtca
    40921 tcaggatacc ggaacctggc agaccggttg ggtgatactg aacgaattcc atgtcacgca
    40981 gcggaacgcc gtggcttagc gccataccca taccgtcacc ggtaacgatg ccgccgttgg
    41041 tgttgtaacg ataaacgcga cccgcaccgc cggtagccat aacgaccgcg ttagcacgga
    41101 tctgcaccag cgtgccttcc atcatgttca ttgctaccag gccgcgaaca tgaccatcat
    41161 caaccagaat atccagcacg aaatgttcgt caaaacgctg gatctgcggg aattgcagag
    41221 aggtctggaa cagcgtgtgc agcatatgga agccggtctt atcggcggcg aaccaggtac
    41281 gctcgatttt catgccgccg aagcgacgta cgttgacgct accatccggg cgacggctcc
    41341 atgggcaccc ccacagttcc agttgggtca tttcggttgg gcagtggtgg acgaaataat
    41401 ccacgacatc ctgctcacac aaccagtcgc cacccgctac tgtatcgtga aagtgatatt
    41461 cgaagctgtc atgatcctgc gcgacagcgg cggagccccc ttctgcagca acggtatggc
    41521 tacgcatcgg gtatactttt gagattagtg cgatttttgc atttggattt gcctgcgcgg
    41581 cagcaattgc agcacgtaat cccgcgccac cggcgcctac aatggcaaga tcggcttgaa
    41641 aggtttgcac gacattcctc cagattgttt ttatcccaca gccccgtact tcagggtaag
    41701 tgcctgaaag ttacggtctg cgaacgctat tccactgctc ctttataggt acaacagtat
    41761 agtctgatgg ataagtctga aatttgacga gatcgatttt tttagtgcgc aagagggcta
    41821 aattatcact gaagatgatt aatttaatta ctaaaccatc agatcgtgct ttttttagtc
    41881 actgcccacc gctgtttgat tcctgcgtaa aatttgtctc gccgtcgcgt tgcgagtaga
    41941 cttcgtgccc ttgtcaaaaa ctggagattt aactatgagc gaaacggcat cctggcagcc
    42001 gagcgcatcc attcctaact tattaaaacg cgcggcgatt atggcggaga tccgtcgttt
    42061 ctttgccgat cgtggagtgc tggaggtgga gacgccttgt atgagccagg cgacggtaac
    42121 cgatattcat ttggtcccgt ttgagacacg tttcgttggc cccgggcatt cgcaggggat
    42181 gaatctctgg ttaatgacca gcccggaata ccatatgaaa cgcctgctgg ttgccggttg
    42241 tgggccggta ttccagctgt gccgcagctt ccgtaatgaa gagatggggc gttatcacaa
    42301 ccctgagttc actatgctgg agtggtatcg accgcactat gatatgtacc ggttgatgaa
    42361 cgaggtggac gatctcttac aacaggtgct ggactgcccg gcagcagaaa gcctttctta
    42421 tcaacaagct ttcttgcgtt atctggaaat tgacccgctc tctgccgaca aaacgcaact
    42481 gcgggaagtc gcagcgaaac tggatttgag caatgttgct gataccgaag aagaccgcga
    42541 cacgctgcta caattgctgt ttacctttgg cgtagagcca aatattggca aagaaaaacc
    42601 gacctttgtg taccactttc cagccagcca ggcatcactg gcgcaaatca gtaccgaaga
    42661 tcatcgggtc gctgaacgct ttgaggttta ttataaaggt attgagctgg cgaatggttt
    42721 ccatgaattg acggatgccc gtgagcagca acaacgcttt gaacaagata accgtaagcg
    42781 cgcggcgcgc ggtttgccgc agcaccccat tgaccagaat ctgattgaag ccttgaaagt
    42841 cggtatgcct gactgttccg gcgtggcatt aggtgttgat cgtctggtga tgttggcgct
    42901 gggcgcggag acactggctg aagtcatcgc ctttagcgtt gaccgggcat aattctgaaa
    42961 aattactgaa ttaacagcga agaatggcgt gataagcgcc attctgtagc atatgttttc
    43021 atttttccgt taaaggccac aatttaagac tggatatttg atatcatcca ggtatcaatt
    43081 ctgtattggt ttttacgctg cctgctcaat gttgcgcagt gcagttcagt gggcacgtgt
    43141 tatacacgcg ctgaaatgaa ggatggtttc atgcctcaca cgataaaaaa gatgagtctg
    43201 ataggactca tattgatgat ctttacttcc gtatttggat ttgccaatag cccatcggct
    43261 tattacttaa tgggttatag tgcgattccc ttttatatat tttctgcatt gttattcttt
    43321 attccattcg ccttaatgat ggctgaaatg ggagctgctt atcgcaaaga agaaggcggt
    43381 atctattcct ggatgaataa tagtgtcgga ccacgttttg ccttcattgg tacgtttatg
    43441 tggttttcct cttatatcat ctggatggtg agtacctccg cgaaagtttg ggtaccgttc
    43501 tcaacattcc tctatggtag cgacatgacc cagcactggc gtattgccgg actggagcct
    43561 acgcaggtgg ttggtctgct ggcagtggca tggatgattc tggtcaccgt cgttgcttct
    43621 aaggggatta ataaaattgc ccgcattact gcggtgggcg gtattgcagt aatgtgtctg
    43681 aatttagtat tgctgttagt aagcattact attttgttat taaatggtgg gcatttcgcg
    43741 caggatatta atttccttgc atcaccgaac ccgggttatc agtccggtct ggcaatgcta
    43801 tcgtttgtgg tatttgccat ttttgcctat ggcggaattg aagcggttgg tggtctggtc
    43861 gataaaacgg aaaatccaga aaagaacttt gccaaaggta ttgtttttgc cgctattgtt
    43921 atttcaatcg gttattcgct ggcaatattt ttatggggcg tcagcacaaa ctggcagcag
    43981 gtattaagta atggttccgt taacctcggc aatattacct atgtgctgat gaagagcctt
    44041 gggatgacgc tgggtaatgc actgcatttg tcacctgaag cgtcattgtc gctgggcgta
    44101 tggtttgcgc gtattactgg actttcgatg ttcctcgcct ataccggtgc gttctttacg
    44161 ctttgctatt caccgttgaa agccatcatc caggggacgc cgaaagcatt gtggccggaa
    44221 ccgatgacgc gcctgaatgc gatggggatg ccgtctatcg ccatgtggat gcagtgcggg
    44281 ttggttactg tcttcatcct gctggtttcg tttggtggcg gtaccgcatc ggcgttcttt
    44341 aacaagctga cgctgatggc gaacgtgtct atgacgcttc cttacctgtt cctcgcgctg
    44401 gctttcccat tctttaaagc acgtcaggat ctcgacagac catttgtgat tttcaaaacg
    44461 cgtatgtcgg caatgatcgc gacggtggtt gtcgtactgg tggtgacatt tgcgaacgtc
    44521 ttcaccatca ttcaacctgt ggttgaagct ggagactggg acagcacatt gtggatgatt
    44581 ggcggccctg tcttcttctc gctgttagcg atggcgattt accagaacta ttgcagcaga
    44641 gtggcaaaaa atccgcagtg ggcggtggaa taacccgatg ccctatcgtc tacctgacga
    44701 tagggcgtaa agacaggaaa tgttatggat gacaccagcc gggacccggc aataaccgaa
    44761 gatgaaatca gggcgctgca attttctgcc ggggatgtag cggaaataga gcagacgatt
    44821 ctctcttttg tcgatgcctg ccacacccgt aaagtggcga tggtggtggg tagcaccatt
    44881 aacaccctca aagatcgcga tggtaaacgg tggggaaacc tgccggatat ttattgcgct
    44941 tatcttatcc gatgtctggt ttttcgcggg gaattagtgg gatacggcga tttgtttcgt
    45001 atgcgctata gcgaaattaa gcgaccggtt accttatgag tgcgcgcatg tttgtcttat
    45061 gctgcatctg gtttattgtg gcgttcctct ggataaccat cacttccgcg ctggacaaag
    45121 agtggatgat tgatggacgc gggattaaca acgtctgcga tgtgttgatg tatctcgaag
    45181 aagacgatac ccgtgatgtt ggtgtgataa tgacgttacc gctctttttc ccttttctct
    45241 ggttcgcttt gtggcgaaaa aaacgcggct ggtttatgta cgccaccgcg ctggctattt
    45301 tcggctactg gctgtggcag ttttttctgc gctatcagtt ttgtttgtga gccggattgg
    45361 ttcatccggc acacaaactt acaaacttcc cgcctgacga cctttgcccg cagacgtcag
    45421 cgttctcccc gtttgtttac cgttgaggct ttccagacgc atctggaagg gcgggaatgg
    45481 catatcgata ccgtgggcat ggaagccagc cagaatcagc tggtggatct catggcgtag
    45541 cggcatacgg tgacccatct cagcggcgta aatacgcagc tcgaaaatct gaatcccctg
    45601 ttgcagatcc accaggaaga cttccggtgc cgggttgtcg atcaccagcg agcagcgacg
    45661 cgctgcggtg agcaggattt ccgtcacttc ttcgctattg gcatcggcag gggccggtat
    45721 cgtcaacacc acgcgcgtga ccgagtcaga gagcgaccag ttgataaact gctcggtaat
    45781 aaacgccttg ttcggcacga ttatctcttt acggtcccag tcgctgatgg tggtggcgcg
    45841 ggtgttaatt ttcgtcacgc taccggtgag atcgcgaatt gtcaccgtat cgccaatgcg
    45901 aatcggtttt tcgaacagga taatcagacc agagataaag ttggcgaaaa tttcctgcaa
    45961 accaaaaccg agaccaacac cgagcgcggc aaccagccac tgcaatttcg accactcaat
    46021 accaatcatt gagaagccga ccagcccgcc aatcagcatc agcagatatt tggtgatggt
    46081 ggtgatggcg taacccgtac ccggcgttaa atccaggtgc tgcaaaatcg ccagttccag
    46141 cagcgcgggt aggttgcgca ccagctgcgt ggtgatgata aataccagaa tggcaattag
    46201 cactgcaccg agggtaatcg gctccaggct ttctacgccc tgtacagtgg aagtgacatc
    46261 ccacagagaa atattttcga ggaagccgaa agcggaatgg atttctgacc acagcacaat
    46321 gaccgaaagc agggcgatca acatcaaaat tgagcgcacc agccgcaagg attgcgcact
    46381 gatggcatcg agatcgactt cgctttcatc gacttcaatt gccccttccg ggctactgtg
    46441 atgatgcgct tcctcttcgc cacgcgcgcg ttgcgctaac atctctgccc ggcgatgctt
    46501 cgcccggtca aacgccagcc tgcgacgctg gatcagcatc cagcggcgga taacgtgata
    46561 aaccaccagt agcaggaacc agatggcaac cgaggtttca agcctcgcta acagcgcctg
    46621 tgccgttgcc agataaccca cagccgacgc cagaatggca accaacggcg cgccaatcat
    46681 catgttccac agcatatggt tggtaatgtt gtcgccgctg ccctctttgt tgagatacag
    46741 tgggatcccg gcctttttca ggctgagggt gaccaccgcc agcgcaccgc aaatgaggat
    46801 aaagcaaagc cgtcccagcg aaccggagaa ttcacggtcg tcgaggttat cgaacatcat
    46861 cagcgccata atcagcggca caataagccc gatgctcatc aggtagtagc gcatcccacg
    46921 ggaaacacgt tcgcgcggcc agccaaaatg agcgataaac aagccgttcg ggcgggcaaa
    46981 ggtggcgcaa atcatcacta cccacagcag cggcacggtg gccgttacac catcgccaat
    47041 cgcgaccgcc agcggataag gccacgcctc gcgcaagccg taacccagcg tcatccacag
    47101 caccggtaac ggtgacgcga cgagaatcga ccagaaaaga gtgcgcaacg tcagccagaa
    47161 gtgatcctga gtcactttgc caactttcgc cgccgaacgt tcaagaaaac gggtgaaata
    47221 gcggcgcgag taaatactgc aaccgaccag aatcaacgcg ccaaacagcg gcaaaattgt
    47281 ctctttgctg gtcagcatca tcacactggc tttgcccaac tgactgaagg tgtccagcga
    47341 aatgagacga cgcagatcct gggcgatttc cagcggccag gcgatggtca tcgggcgcac
    47401 gtcagaggtc cagaacagat agcggtgcgt tgcttcgttc acctctttca gcgcatcctc
    47461 cagttgcccg ttggagactt tcagcttggt cagttccaat agtagcgtgt cgccaccctg
    47521 caacaatgag ttcagcaact cacgctgagt gcgcagttgt gcttccagaa tacggttttg
    47581 ctcggcagtc agcggctgac cgtcggcctg atgaatttgc cgtagcagcg gctgtttatt
    47641 aagcagatcc tcataacgta accgttgcac acgcaactgc gccatttcgg tatcaagctg
    47701 ttgtggtttc ggcatttccg gcagccgtgc cacctgcgcc cgcagcgctt cgccgagcag
    47761 attggacgat cccagccatt gcgactgttc acgcagcgta ttcaacgcct gccggacctg
    47821 taacgtctgg ctggcagcct gacgctgttg cgaggcaacg agatccatcc gctgcgcctg
    47881 ttgattcaaa gccgccgata gttcgcggtt aattttgaat tgcgcgacga tatctttcgg
    47941 caaatcggcg ctgttttctg ccagcaattc ggtactttcc agcgcccgct ccgcctcaag
    48001 ctgacgttgg ctgtttaatt gattacgcaa ggcctgcaaa tacgcatcca gttgctggct
    48061 ctctttttcc gccagctctg agcgtaagcg cgctaattcc tggcggttat tggcagacag
    48121 ctgcgccagc tccagttcat caacgagcgc cttaagacgt gcagagtcag actgcaacgc
    48181 gaaattttgt gcctgattga gcggagtatt gccggtaagc gttcccaggc ggcgctcgat
    48241 ctcatttaac tgacggcggg cgtcggtttg ctgttgcggc agttgattca gcgaatcggc
    48301 aatctcgcgg gcgcgctcct gctcttgctg ggcctgacgg cttttatcca gcaactggct
    48361 gctgacctgg agaatttcct gattcagcgc gtcggtagac attcccggcg acacgctgcg
    48421 cggctcgtca cgcatgttgt ttaattgtgc gcgcagagta gcggagagtt tcggataatt
    48481 atcgataact tgctgatatt gtttgatgcg ctcaagggaa ccttttcgtt cctcaagcgc
    48541 atttaaggca gactggagcg cctctacgac ttccggctgt gcgggtttcg ccgcttttgc
    48601 ctgctccagt tcctgagtga tttgtttgct atcgggggcc gtcgcggcgt acgcccccca
    48661 actgaggcac caggccatca gaaaagtgat aatcaggcgc acgtcagcgt ttcctttgat
    48721 ggattagacc tggtcttttt tgtcgtcaac caatgggctg gcgtcgtgtt ctgcttcgat
    48781 ctcttcagca ggaagcgggg caggttcagc gtctggcgta acaaaggttt cggtagatac
    48841 tgccagcggc tggccaattt tcgtgacaga caggctttcc agttgctcaa ccagattcac
    48901 tttacccggt gcaaacaggt tgataacggt ggaaccgagt ttaaagcgac ccatttcctg
    48961 gcctttcagc agtgccacag aaccgtcgtt ttccccggca ggccaggtcc agcgcttgat
    49021 gataccttcg cgcggcggcg taatggtgcc cgcccagacc gtctcaatgc tgccaacaat
    49081 cgtcgctccg accagaatct gcgccattgg gccaaattcg gtatcgaaaa ggcaaatcac
    49141 gcgttcgtta cgggcaaaca gattcggcac gttctgagcc gtgagatggt taacggagaa
    49201 gagatcgccc ggcacgtaga tcatctcacg cagaataccg ttgcacggca tgtgtacgcg
    49261 gtggtagtca cgtggggaga ggtaagtggt cacaaacgta ccgttgcgga acaggtccgc
    49321 catcagatag ttgcctgcca gcagggcttc gaggctgtag ttgtggcctt tggcttgcag
    49381 gattttatct tcttcgattt tacccaactg gctgataacg ccatcggcag gcatgaccag
    49441 tacattcgga tcggtatcga ttgggcgtac ttcgtcacgc agcggacgga caaagaattc
    49501 gttaaaggtg cggtagctgg cggtgtccgg cttttgcgcc tctttcatgt cgaccttgta
    49561 gtatttaacg aacagatcga taaccagttt tgtcagccat cctgcccgct tgcttgcgcc
    49621 ccaacccgcc aggcgagtaa gccatagttt cggcagaatg tactgtagcg aaagtttaaa
    49681 tgaatttaac aaggtagcct ccaggccatt gttttgtcgt tcctgatccg gcctacatgc
    49741 cggatcctga aaaaaagggg acgattctaa cgacggttag cttaattgtc agtcatccgt
    49801 atcagaaaag tttttacgcg tttttacctg cgccatgctt tccagaatac ggtgatagtt
    49861 ttcgaaacgg gtttccgcga ttttcccttc ctcaaccgct tcccggatag cgcagcccgg
    49921 atcggtatcg tgtttgcaat cgcgatattt acacagacct aaatagtcat ggaattcgac
    49981 aaagccctga gtgatttgtt ccggctccag gtgccagagg ccgaactcac gcactcctgg
    50041 ggagtcaatc acatcaccgc cgtgcgggaa gtgatacagc cgagcggcgg tcgtggtgtg
    50101 ctgaccgagc cccgagttgt cagagacatc gttggtcagg atctcttttt gcagccccag
    50161 caacgcattc agcaggctgg atttgccgac gccagactgc ccggcaaaaa tgctgatgcg
    50221 cccggtcaac gcctcttcca gcggttttag cccatcctga gtatggctgg aaaccatcaa
    50281 tacgcgataa ccgatattgc ggtagatatc catctgctcg ttgacgaacg ccatgccttc
    50341 gtcgtccagc aggtctatct tgttgagcac aataatcggc tcaatctgca aggtttcgca
    50401 ggccaccagg taacggtcga taatattgag cgacagctcc ggcaaaatgg cggagacaat
    50461 gacaatctgg tcgatgttgg cggcaatagg ttttacgccg tcgtagaaat ccggacgcgt
    50521 caacaccgag gtacgctcat gcaccgcttc cacgatccct ttgacattta cgccttccgc
    50581 cgccggttta cccggacgcc agactacgcg gtcgccggtt accagcgaac ggatggtacg
    50641 gcgaatattg cagcggtgaa cgtcgccatc ggcggattcc acatcagcgt gcataccaaa
    50701 gcggctgatg acgatacctt catcaggctc gccaaacaga ttgtcgtcgt agtcgggctt
    50761 ctccttagac gttttaagac gacgctggtg attggcgttc acgcggcgct gctggccttt
    50821 ggagagttta tttttactca atcgtacaga ctcctggtcg cccctgatgg gcaaaacatc
    50881 tatgatacac gcaattgtgg atcaatatag tcactgtgaa tgggtggaaa atagcatgag
    50941 tgccaatgaa aacaacctga tttggatcga tcttgagatg accggtctgg atcccgagcg
    51001 cgatcgcatt attgagattg ccacgctggt gaccgatgcc aacctgaata ttctggcaga
    51061 agggccgacc attgcagtac accagtctga tgaacagctg gcgctgatgg atgactggaa
    51121 cgtgcgcacc cataccgcca gtgggctggt agagcgcgtg aaagcgagca cgatgggcga
    51181 tcgggaagct gaactggcaa cgctcgaatt tttaaaacag tgggtgcctg cgggaaaatc
    51241 gccgatttgc ggtaacagca tcggtcagga ccgtcgtttc ctgtttaaat acatgccgga
    51301 gctggaagcc tacttccact accgttatct cgatgtcagc accctgaaag agctggcgcg
    51361 ccgctggaag ccggaaattc tggatggttt taccaagcag gggacgcatc aggcgatgga
    51421 tgatatccgt gaatcggtgg cggagctggc ttactaccgc gagcatttta tcaagctgta
    51481 aaatttttcc tggtcacgta agcgccggaa tccacttgcg gcggcgtgat tttgacgcta
    51541 aattgaacgt cttgctgata atttcagcac ttgagataaa aacgcaaaaa aaactttttt
    51601 ggggggttgc agagggaaag atttctcgta taatgcgcct cccgtaacga cgcagaaatg
    51661 cgaaaattac gaaagcaaaa ttaagtagta cgcgggaata gctcagttgg tagagcacga
    51721 ccttgccaag gtcggggtcg cgagttcgag tctcgtttcc cgctccaaaa tttgaaaagt
    51781 gctgcaaagc acagaccacc caatcgcagt atttcgaatt gcgacaaggc ggcaactgaa
    51841 cgaatccttg ggaacttaca aaagtatgtg actaaggtga gtgaaggcag ccaaggcagt
    51901 agcggttcga aagacgaaga ttaagcggga atagctcagt tggtagagca cgaccttgcc
    51961 aaggtcgggg tcgcgagttc gagtctcgtt tcccgctcca aaatttgaaa gtgctgtaaa
    52021 gcacagacca cccaagcggg aatagctcag ttggtagagc acgaccttgc caaggtcggg
    52081 gtcgcgagtt cgagtctcgt ttcccgctcc aaattcttct ctcaataaaa tatccacagc
    52141 gatgcgatgc gttattgctg gttttcgttg tctctgacaa actcttgtaa acagagttat
    52201 ccacagcctc aggctgtaat cttaatttca aagaaacttc gcacggtgaa tagtattttt
    52261 ttaacctatt gatagataag ttaaaaatta agattccgtt ttgtcgagtc gatcacttga
    52321 cgattttatt cgtcttgaat tgcaatgcgt ttttattttt attcacaagc tgtggatgaa
    52381 tcaggcgtca cgcggtaacc ctttttcaat cacccgaacc agacgctgtt ttttcggcaa
    52441 ttgcacttcg actatgcacg catttcgtct ctctatttgc tgcgcaatcg cccacgctat
    52501 gtgctcatcg agaagtgggt gctcaccttt acgactttcc agcgctgtca aaatcgtttc
    52561 atcccaaggg gcattgccta atgctacggc gatattacgc agccaacgca ggtgaccaat
    52621 acgacgaatc gccgatcctt ccgtgacttt taaaaacttc tcttcgctcc aggcgaataa
    52681 ctcaatgagt tccggtgcgt gtagcggctt acgcgggctg aaatcctctt ctgtggtgag
    52741 ttgtgaatag cgattccacg ggcagataag ctggcagtca tcgcaaccgt aaatacggtt
    52801 tcccattaac ggtcgcaact cttccgggat cgccccttca agttcgatgg tgagataaga
    52861 gatacagcgg cgagcatcga cggtatatgg ctcgacgatg gcaccggtcg ggcaaatcgt
    52921 catacaggcc acgcatttgc cgcatccttc ctcgactggt tgatccacgg gcagcggaat
    52981 atcgaccagc aattcgccta aaaagaagaa cgaaccggcc tcgcgattga ggataagtga
    53041 gtgcttacct gtccagccga gcccagcttt ttcagctaac gggcgctcga gaataggcgc
    53101 agaatcgaca aacggtctaa aattcagcga aacacaatgt tgctgaatca tctcgcccag
    53161 ctttttgagt cggttgcgca gaagtttgtg atagtcacgg cccagcgcat aacggctaac
    53221 atagccgagt ttggggtttt tcagcgtgct ggcaaatgcg gcgttagcag gaaggtaatt
    53281 catccgcacg ctgatcacgc gcagcgtacc gggcaataac tcatgagggc gagcgcgcag
    53341 cataccgtga cgtgccatcc agtccatttc gccgtggtat tgtttgtcca gccatgcttg
    53401 cagtttgggc tcggactcgc tgagatcggt atcggtaata cctacctgct gaaagcccag
    53461 ttccagcccc cactgtttaa ttttttgcgc taactgattg agatcgaggg gctctgacat
    53521 gacggaccat acaatgaaga aaaaccccgt aagtatacca cacaccgtct ggtacgccga
    53581 cgatatccgc cgcggagaac gcgaggcggc agatgtgctg gggctcacac tctatgagct
    53641 gatgcttcgc gctggcgagg ccgcattcca ggtgtgtcgt tcggcgtatc ctgacgcccg
    53701 ccactggctg gtgctgtgcg gtcatggtaa taacggcggc gatggctacg tggtcgcgcg
    53761 actggccaaa gcggtcggca ttgaggtcac gttgttggcc caggagagcg acaaaccgtt
    53821 gccggaagag gccgcgctgg cacgcgaagc atggttaaac gcgggtggcg agatccatgc
    53881 ttcgaatatt gtctggcccg aatcggtaga tctgattgtt gatgcgctgc tcggtaccgg
    53941 tttgcggcaa gcgccccgcg aatccattag ccagttaatc gaccacgcta attcccatcc
    54001 tgcgccgatt gtggcggttg atatcccttc cggcctgctg gctgaaactg gcgctacgcc
    54061 aggcgcggtg atcaacgccg atcacaccat cacttttatt gcgctgaaac caggcttgct
    54121 cactggaaaa gcgcgggatg ttaccggaca actgcatttt gactcactgg ggctggatag
    54181 ttggctggca ggtcaggaga cgaaaattca gcggttttca gcagaacaac tttctcactg
    54241 gctaaaaccg cgtcgcccga cttcgcataa aggcgatcac gggcggctgg taattatcgg
    54301 tggcgatcac ggcacggcgg gggctattcg tatgacgggg gaagcggcgc tgcgtgctgg
    54361 tgctggttta gtccgagtac tgacccgcag tgaaaacatt gcgccgctgc tgactgcacg
    54421 accggaattg atggtgcatg aactgacgat ggactctctt accgaaagcc tggaatgggc
    54481 cgatgtggtg gtgattggtc ccggtctggg ccagcaagag tgggggaaaa aagcactgca
    54541 aaaagttgag aattttcgca aaccgatgtt gtgggatgcc gatgcattga acctgctggc
    54601 aatcaatccc gataagcgtc acaatcgcgt gatcacgccg catcctggcg aggccgcacg
    54661 gttgttaggc tgttccgtcg ctgaaattga aagtgaccgc ttacattgcg ccaaacgtct
    54721 ggtacaacgt tatgacggcg tagcggtgct gaaaggtgcc ggaaccgtgg tcgccgccca
    54781 tcctgacgct ttaggcatta ttgatgccgg aaatgcaggc atggcgagcg gcggcatggg
    54841 cgatgtgctc tctggtatta ttggcgcatt gcttgggcaa aaactgtcgc cgtatgatgc
    54901 agcctgtgca ggctgtgtcg cgcacggtgc ggcagctgac gtactggcgg cgcgttttgg
    54961 aacgcgcggg atgctggcaa ccgatctctt ttccacgcta cagcgtattg ttaacccgga
    55021 agtgactgat aaaaaccatg atgaatcgag taattccgct ccctgatgag caggcaacat
    55081 tagacctggg cgagcgggta gcgaaagcct gcgatggcgc aaccgtaatc tatctgtatg
    55141 gcgatttagg cgcaggtaaa accaccttta gccggggctt tttacaggct ctgggtcatc
    55201 agggtaatgt caaaagcccc acttatacgc tggtcgaacc ctatacgctc gacaacttaa
    55261 tggtctatca ctttgatttg taccgccttg ccgatcccga ggagctggag tttatgggga
    55321 tccgcgatta ttttgccaac gatgccatct gcctggtgga gtggccacaa caaggtacag
    55381 gtgttcttcc tgacccggat gtcgaaatac acattgatta tcaggcacaa ggccgtgagg
    55441 cgcgcgtgag tgcggtttcc tctgcgggtg aattgttgct ggcgcgttta gccggttaac
    55501 ctttgaaagg tggcgggatg atgtatcgca tcagaaattg gttggtagcg acgctgctgc
    55561 tgctgtgcac gccggtgggt gccgcgacgc tctctgatat tcaggtttct aacggtaatc
    55621 aacaggcgcg gataacgttg agttttattg gcgatcctga ttatgcgttt agccatcaaa
    55681 gcaaacgcac cgtggcgctc gatatcaaac aaacgggcgt gattcaggga ctgccgttgt
    55741 tgttcagcgg caataatctg gtgaaggcga ttcgctctgg aacgcctaaa gatgcacaaa
    55801 cgctacggct ggtggtcgat cttaccgaaa acggtaaaac cgaagcggtg aagcggcaga
    55861 atggcagcaa ttacactgtc gtctttacga ttaacgccga tgtgccgcca ccgcctcctc
    55921 cgccgcccgt ggttgcgaaa cgcgttgaaa cgcctgcggt tgtcgcaccg cgcgtcagcg
    55981 aaccggcgcg caatccgttt aaaacggaaa gtaaccgcac tacgggtgtt atcagcagta
    56041 atacggtaac gcgtccggca gcgcgcgcga cggctaacac tggcgataaa attatcatcg
    56101 ctattgatgc cggacacggc ggtcaggacc ctggcgctat cggccccggt ggtacgcggg
    56161 agaaaaatgt caccatcgcc atcgcgcgta aattgcgtac tttgctcaat gacgatccga
    56221 tgtttaaagg cgttttaacc cgtgacgggg attactttat ctcggtgatg gggcgcagtg
    56281 atgtggcacg taagcaaaac gccaatttcc tcgtgtcgat tcacgctgat gccgcaccga
    56341 accgcagtgc gactggcgct tccgtatggg tgctctctaa ccgtcgcgcc aacagtgaaa
    56401 tggccagctg gctggagcag cacgagaaac agtcggagct gctgggtggg gcgggtgatg
    56461 tgctggcgaa cagtcagtct gacccctatt taagccaggc ggtgctggat ttacagttcg
    56521 gtcattccca gcgggtaggg tatgatgtag cgaccagtat gatcagtcag ttgcaacgca
    56581 ttggcgaaat acataaacgt cgaccagaac acgccagcct tggcgttctg cgctcgccgg
    56641 atatcccatc agtactggtc gaaaccggtt ttatcagcaa caacagcgaa gaacgtttgc
    56701 tggcgagcga cgattaccaa caacagctgg cagaagccat ttacaaaggc ctgcgcaatt
    56761 acttccttgc gcatccgatg caatctgcgc cgcagggtgc aacggcacaa actgccagta
    56821 cggtgacgac gccagatcgc acgctgccaa actaaggacg attgatgcca attcaggtct
    56881 taccgccaca actggcgaac cagattgccg caggtgaggt ggtcgagcga cctgcgtcgg
    56941 tagtcaaaga actagtggaa aacagcctcg atgcaggtgc gacgcgtatc gatattgata
    57001 tcgaacgcgg tggggcgaaa cttatccgca ttcgtgataa cggctgcggt atcaaaaaag
    57061 atgagctggc gctggcgctg gctcgtcatg ccaccagtaa aatcgcctct ctggacgatc
    57121 tcgaagccat tatcagcctg ggctttcgcg gtgaggcgct ggcgagtatc agttcggttt
    57181 cccgcctgac gctcacttca cgcaccgcag aacagcagga agcctggcag gcctatgccg
    57241 aagggcgcga tatgaacgtg acggtaaaac cggcggcgca tcctgtgggg acgacgctgg
    57301 aggtgctgga tctgttctac aacaccccgg cgcggcgcaa attcctgcgc accgagaaaa
    57361 ccgaatttaa ccacattgat gagatcatcc gccgcattgc gctggcgcgt ttcgacgtca
    57421 cgatcaacct gtcgcataac ggtaaaattg tgcgtcagta ccgcgcagtg ccggaaggcg
    57481 ggcaaaaaga acggcgctta ggcgcgattt gcggcaccgc ttttcttgaa caagcgctgg
    57541 cgattgaatg gcaacacggc gatctcacgc tacgcggctg ggtggccgat ccaaatcaca
    57601 ccacgcccgc actggcagaa attcagtatt gctacgtgaa cggtcgcatg atgcgcgatc
    57661 gcctgatcaa tcacgcgatc cgccaggcct gcgaagacaa actgggggcc gatcagcaac
    57721 cggcatttgt gctgtatctg gagatcgatc cgcatcaggt ggacgtcaac gtgcaccccg
    57781 ccaaacacga agtgcgtttc catcagtcgc gtctggtgca tgattttatc tatcagggcg
    57841 tgctgagcgt gctacaacag caactggaaa cgccgctacc gctggacgat gaaccccaac
    57901 ctgcaccgcg ttccattccg gaaaaccgcg tggcggcggg gcgcaatcac tttgcagaac
    57961 cggcagctcg tgagccggta gctccgcgct acactcctgc gccagcatca ggcagtcgtc
    58021 cggctgcccc ctggccgaat gcgcagccag gctaccagaa acagcaaggt gaagtgtatc
    58081 gccagctttt gcaaacgccc gcgccgatgc aaaaattaaa agcgccggaa ccgcaggaac
    58141 ctgcacttgc ggcgaacagt cagagttttg gtcgggtact gactatcgtc cattccgact
    58201 gtgcgttgct ggagcgcgac ggcaacattt cacttttatc cttgccagtg gcagaacgtt
    58261 ggctgcgtca ggcacaattg acgccgggtg aagcgcccgt ttgcgcccag ccgctgctga
    58321 ttccgttgcg gctaaaagtt tctgccgaag aaaaatcggc attagaaaaa gcgcagtctg
    58381 ccctggcgga attgggtatt gatttccagt cagatgcaca gcatgtgacc atcagggcag
    58441 tgcctttacc cttacgccaa caaaatttac aaatcttgat tcctgaactg ataggctacc
    58501 tggcgaagca gtccgtattc gaacctggca atattgcgca gtggattgca cgaaatctga
    58561 tgagcgaaca tgcgcagtgg tcaatggcac aggccataac cctgctggcg gacgtggaac
    58621 ggttatgtcc gcaacttgtg aaaacgccgc cgggtggtct gttacaatct gttgatttac
    58681 atccggcgat aaaagccctg aaagatgagt gatatcagta aggcgagcct gcctaaggcg
    58741 atttttttga tggggccgac ggcctccggt aaaacggcgt tagccattga gctgcgtaaa
    58801 attttaccag tagagttgat aagcgttgat tctgccctta tttacaaagg gatggatatc
    58861 gggacggcga agccgaacgc tgaagagtta ctcgccgcgc cgcaccgatt gctggatatt
    58921 cgcgatccgt cgcaggctta ctcggctgct gattttcgcc gcgatgcgct ggcggaaatg
    58981 gccgatatca ccgcggcggg gcggatccca ctgttagtgg gcggtacgat gttgtatttc
    59041 aaggcattgc tggaagggtt gtcgccgcta ccgtcggcag acccggaagt acgggccaga
    59101 attgagcaac aggcggcaga gcaaggttgg gagtcattgc atcgtcaact tcaggaggta
    59161 gatccggttg cggcagcaag gattcatcca aatgatccac aaaggctttc ccgggcactg
    59221 gaagtttttt tcatttcggg taaaacttta acggaactga cgcaaacgtc aggagacgct
    59281 ctaccgtatc aggtgcatca gttcgccatc gccccggcga gccgtgaact gctccatcaa
    59341 cgcattgagc agcgttttca tcagatgttg gcttcaggtt ttgaagcaga agtccgggcg
    59401 ctttttgccc gaggagattt gcatacggac ttgccttcca ttcgttgcgt gggttatcgc
    59461 cagatgtggt cttaccttga aggcgaaatc tcatacgatg aaatggttta tcgaggtgtt
    59521 tgcgccacga gacagttggc gaagcggcag ataacctggc tgcgtggttg ggaaggggtt
    59581 cactggcttg acagtgaaaa accagaacag gcgcgtgacg aagtattaca ggttgttggt
    59641 gctatcgcag gctgaatgtg tacaattgag acgtatcgtg cgcaattttt tcagaatcga
    59701 aaggttcaaa gtacaaataa gcatataagg aaaagagaga atggctaagg ggcaatcttt
    59761 acaagatccg ttcctgaacg cactgcgtcg ggaacgtgtt ccagtttcta tttatttggt
    59821 gaatggtatt aagctgcaag ggcaaatcga gtcttttgat cagttcgtga tcctgttgaa
    59881 aaacacggtc agccagatgg tttacaagca cgcgatttct actgttgtcc cgtctcgccc
    59941 ggtttctcat cacagtaaca acgccggtgg cggtaccagc agtaactacc atcatggtag
    60001 cagcgcgcag aatacttccg cgcaacagga cagcgaagaa accgaataag gtttcgggct
    60061 gtttttttac acggggagcc agcgatcctg cgttccccgc tgatctattt agagggttat
    60121 acgcttgttt gaccgttatg atgctggtga gcaggcggta ctggtacaca tctattttac
    60181 gcaagacaaa gatatggaag acctccagga gtttgaatct ctggtctctt ccgccggtgt
    60241 cgaagcattg caggtgatta ccggtagccg taaagcgccg cacccaaagt attttgtagg
    60301 tgaaggtaaa gcagttgaaa ttgcggaagc tgtcaaagcg acgggtgctt cggtcgttct
    60361 ttttgaccat gccctgagcc cggcgcaaga gcgtaacctg gagcgtttgt gcgagtgtcg
    60421 tgttatcgac cgcaccggcc ttattttaga tattttcgcc caacgtgcgc gtacccatga
    60481 gggtaagttg caggttgagc tggcgcagct gcgccatctg gctacgcgcc tggtgcgtgg
    60541 ctggacccac cttgaaagac agaaaggcgg gataggtttg cgtggtccgg gtgaaaccca
    60601 gctcgaaacc gaccgtcgtt tgttgcgtaa tcgcatcgtg cagatacagt cgcgcctgga
    60661 aagagttgaa aagcagcgtg agcaggggcg gcaatcgcgt atcaaagccg acgttcctac
    60721 tgtttcgctg gtgggatata ccaacgccgg taaatctacc cttttcaatc gcatcaccga
    60781 agcgcgggtc tacgcggcag accagttgtt tgccaccctc gacccgacgt tgcggcgtat
    60841 tgacgttgca gatgtcggtg aaaccgtact tgcagatacc gtagggttta ttcgccacct
    60901 gccgcacgat ctggtggcgg catttaaagc cacgttacaa gagacgcggc aagccacatt
    60961 actgctgcac gtcattgatg cggcggatgt gcgtgtacaa gaaaacatcg aagcggtgaa
    61021 tacggttctt gaagagatcg acgctcacga gatcccaacc ctgctggtga tgaacaagat
    61081 cgatatgctg gaagatttcg aaccgcgtat tgatcgggac gaagagaaca aaccgatccg
    61141 tgtctggctt tccgcacaga ccggagcggg gataccacag ctttttcagg ctttgacgga
    61201 gcggctttcc ggcgaggtgg cgcagcatac attgcgtctg ccaccgcagg aagggcgtct
    61261 gagaagtcgt ttttatcagc ttcaggcaat agaaaaagag tggatggagg aggacggcag
    61321 cgtaagtctg caagttcgta tgccgatcgt tgactggcgt cgcctctgta aacaagaacc
    61381 ggcgttgatc gattacctga tctaacggcg tagcgtctga agcgtggagt catatcctct
    61441 ggcgtcgaaa gacaacaggg atcaccgcat aacaaatatg gagcacaaac atggcgtgga
    61501 atcagcccgg taataacgga caagaccgcg acccgtgggg aagcagcaaa cctggcggca
    61561 actctgaggg aaatggaaac aaaggcggtc gcgatcaagg gccacctgat ttagatgata
    61621 tcttccgcaa actgagcaaa aagctcggtg gtctgggcgg cggtaaaggc accggatctg
    61681 gcggtggcag ttcatcgcaa ggcccgcgcc cgcagcttgg cggtcgtgtc gttaccatcg
    61741 cagcggcagc gattgtcatt atctgggcgg ccagtggttt ctataccatt aaagaagccg
    61801 aacgcggcgt ggtaacacgc tttggtaaat tcagccatct ggttgagccg ggtctgaact
    61861 ggaaaccgac gtttatcgac gaagtcaaac cggtgaacgt ggaagccgtg cgtgaactgg
    61921 ccgcttctgg tgtgatgctg acgtcggacg agaacgtagt gcgcgttgag atgaacgtgc
    61981 agtaccgcgt caccaatccg gaaaaatatc tgtatagcgt gaccagcccg gatgacagcc
    62041 tgcgtcaggc taccgacagc gccctgcgtg gagttatcgg taaatacacc atggaccgca
    62101 ttctgacgga aggtcgtacc gtgattcgta gcgatactca gcgcgaactg gaagagacga
    62161 ttcgtccgta tgacatgggt atcacgctgc tggacgtcaa cttccaggct gctcgtccgc
    62221 cggaagaagt aaaagcggcg tttgacgatg cgattgccgc gcgtgaaaac gaacagcaat
    62281 acattcgtga agcagaagcg tataccaacg aagttcagcc gcgtgcgaac ggtcaggcgc
    62341 aacgtatcct cgaagaggcg cgtgcgtaca aggcccagac catcctggaa gctcagggtg
    62401 aagtggcgcg ctttgctaaa cttctgccgg aatataaagc cgcgccggaa attactcgcg
    62461 agcgtctgta tatcgagacg atggaaaaag tgttgggtaa cacccgcaaa gtgctggtta
    62521 acgataaagg tggcaacctg atggttctgc cgttagacca gatgctgaaa ggtggtaacg
    62581 cccctgcggc gaagagcgat aacggtgcca gcaatctgct gcgtctgccg ccagcctctt
    62641 cctccacaac cagtggagca agcaacacgt cgtccaccag tcagggcgat attatggacc
    62701 aacgccgcgc caacgcgcag cgtaacgact accagcgtca gggggaataa cgatgcgtaa
    62761 gtcagttatc gcgattatca tcatcgtgct ggtagtgctt tacatgtctg tctttgtcgt
    62821 caaagaaggt gagcgcggta ttacgctgcg ttttggtaag gtactgcgtg acgatgacaa
    62881 caaacctctg gtttatgagc cgggtctgca tttcaagata ccgttcattg aaacggtgaa
    62941 aatgctcgac gcacgtattc agaccatgga caaccaggcc gaccgctttg tgaccaaaga
    63001 gaagaaagac ctgatcgtcg actcttacat caaatggcgc atcagcgatt tcagccgtta
    63061 ctacctggca acgggtggtg gcgacatttc gcaagcggaa gtgctgttga aacgtaagtt
    63121 ctctgaccgt ctgcgttctg aaattggtcg cctggacgtg aaagatatcg tcaccgattc
    63181 ccgtggtcgt ctgaccctcg aagtacgtga cgcgctgaac tccggttctg cgggtacaga
    63241 agatgaagtt actaccccgg cggcagataa cgccattgcc gaagcggcag agcgcgtaac
    63301 ggctgagacg aagggcaaag ttccggtcat caacccgaac agtatggcgg cgctgggtat
    63361 tgaagttgtc gatgtgcgaa ttaagcagat caacctgcct accgaagtgt ctgaagcgat
    63421 ctacaaccgt atgcgcgccg agcgtgaagc ggtagcgcgt cgtcaccgtt cacaaggtca
    63481 ggaagaagcg gaaaaactgc gcgcgactgc cgactatgaa gtgaccagaa cgctggcaga
    63541 agctgagcgt cagggccgta tcatgcgtgg tgaaggcgat gccgaagcag ccaaactgtt
    63601 tgctgatgca ttcagtaaag atccggactt ctacgcattc atccgtagcc tgcgtgctta
    63661 tgagaacagc ttctctggca atcaggacgt gatggtcatg agcccggata gcgatttctt
    63721 ccgctacatg aagacgccga cttccgcaac gcgttaatat aacgactgcg gtacaggtca
    63781 ataaagccac cgcatcctca gggatgtcgg tggttttctt tttctataag gataatgaat
    63841 gaattcgaca atctggctgg cgcttgccct ggttttggta ctggaaggtt tagggccgat
    63901 gctttacccg aaggcatgga agaagatgat ctctgcgatg accaatttgc ccgataatat
    63961 tttacgtcgt tttggcggtg gacttgtggt tgcgggcgtt gtggtctact acatgttgag
    64021 gaaaacgatt ggctgaacaa aaaacagact gatcgaggtc atttttgagt gcaaaaagtg
    64081 ctgtaactct gaaaaagcga tggtagaatc catttttaag caaacggtga ttttgaaaaa
    64141 tgggtaacaa cgtcgtcgta ctgggcaccc aatggggtga cgaaggtaaa ggtaagatcg
    64201 tcgatcttct gactgaacgg gctaaatatg ttgtacgcta ccagggcggt cacaacgcag
    64261 gccatactct cgtaatcaac ggtgaaaaaa ccgttctcca tcttattcca tcaggtattc
    64321 tccgcgagaa tgtaaccagc atcatcggta acggtgttgt gctgtctccg gccgcgctga
    64381 tgaaagagat gaaagaactg gaagaccgtg gcatccccgt tcgtgagcgt ctgctgctgt
    64441 ctgaagcatg tccgctgatc cttgattatc acgttgcgct ggataacgcg cgtgagaaag
    64501 cgcgtggcgc gaaagcgatc ggcaccaccg gtcgtggtat cgggcctgct tatgaagata
    64561 aagtagcacg tcgcggtctg cgtgttggcg accttttcga caaagaaacc ttcgctgaaa
    64621 aactgaaaga agtgatggaa tatcacaact tccagttggt taactactac aaagctgaag
    64681 cggttgatta ccagaaagtt ctggatgata cgatggctgt tgccgacatc ctgacttcta
    64741 tggtggttga cgtttctgac ctgctcgacc aggcgcgtca gcgtggcgat ttcgtcatgt
    64801 ttgaaggtgc gcagggtacg ctgctggata tcgaccacgg tacttatccg tacgtaactt
    64861 cttccaacac cactgctggt ggcgtggcga ccggttccgg cctgggcccg cgttatgttg
    64921 attacgttct gggtatcctc aaagcttact caactcgtgt aggtgcaggt cctttcccga
    64981 ccgaactgtt tgatgaaact ggcgagttcc tctgcaagca gggtaacgaa ttcggcgcaa
    65041 ctacggggcg tcgtcgtcgt accggctggc tggacaccgt tgccgttcgt cgtgcggtac
    65101 agctgaactc cctgtctggc ttctgcctga ctaaactgga cgttctggat ggcctgaaag
    65161 aggttaaact ctgcgtggct taccgtatgc cggatggtcg cgaagtgact accactccgc
    65221 tggcagctga cgactggaaa ggtgtagagc cgatttacga aaccatgccg ggctggtctg
    65281 aatccacctt cggcgtgaaa gatcgtagcg gtctgccgca ggcggcgctg aactacatca
    65341 agcgtattga agagctgact ggtgtgccga tcgatatcat ctctaccggt ccggatcgta
    65401 ctgaaaccat gattctgcgc gatccgttcg acgcgtaatt ctggtacgcc tggcagatat
    65461 tttgcctgcc gggctaacag tgtgatacat tgctgtgtcg gttaagccat tacgctatcc
    65521 gacacagtgt taaatcctcg cttttttcct tccccgaact gaaataaatt agcgacacag
    65581 cttgtggctg gtttatcatc aatataaatg tattttttcc cgatttccct tttgaggttg
    65641 atgtgcagtt aacgagtttc actgattacg gattacgtgc gctgatctac atggcgtcat
    65701 tgccagaagg gcggatgacc agtatttctg aagtgactga cgtctacggc gtctcccgta
    65761 atcatatggt caaaataatc aatcaactta gtcgtgccgg ctacgtgact gctgttcgtg
    65821 gaaaaaatgg cggcattcgc ctgggtaaat cggcgagtgc gatacgtatt ggtgatgtgg
    65881 tgcgcgagct ggagccctta tcgctggtga attgcagcag tgagttttgc cacattacac
    65941 ctgcctgtcg gttgaaacag gcgctttcta aggccgtgca aagttttctt acggaactgg
    66001 ataactacac gcttgccgat ttggttgaag agaatcaacc gctttataaa ttattgctgg
    66061 tggagtgacg aaaatcttca tcagagatga caacggagga accgagatgt cacaagatcc
    66121 tttccaggaa cgcgaagctg aaaaatacgc gaatcccatc cctagtcggg aatttatcct
    66181 cgaacattta accaaacgtg aaaaaccggc cagccgtgat gagctggcgg tagaactgca
    66241 cattgaaggc gaagagcagc ttgaaggcct gcgtcgccgc ctgcgcgcga tggagcgcga
    66301 tggtcaactg gtcttcactc gtcgtcagtg ctatgcgctg ccggaacgcc tcgacctggt
    66361 gaaaggtacc gttattggcc accgtgatgg ctacggcttt ctgcgggttg aagggcgtaa
    66421 agatgatttg tatctctcca gcgagcagat gaaaacctgc attcatggcg atcaggtgct
    66481 ggcgcagccg ctgggcgctg accgtaaagg tcgtcgtgaa gcgcgtattg tccgcgtact
    66541 ggtgccaaaa accagccaga ttgttggtcg ctactttact gaagcgggcg tcggctttgt
    66601 ggttcctgac gatagccgtc tgagcttcga tatcttaatc ccgcccgatc agatcatggg
    66661 cgcgcggatg ggctttgtgg tcgtagtcga actgactcag cgtccgactc gccgcaccaa
    66721 agcggtgggt aaaatcgtcg aagtgctggg cgacaatatg ggcaccggca tggcggttga
    66781 tatcgctctg cgtacccatg aaattccgta catctggccg caggctgttg agcaacaggt
    66841 tgccgggctg aaagaagaag tgccggaaga agcaaaagcg ggccgtgttg atctgcgcga
    66901 tttaccgctg gtcaccattg atggcgaaga cgcccgtgac tttgacgatg cagtttactg
    66961 cgagaaaaaa cgcggcggcg gctggcgttt atgggtcgcg attgccgacg tcagctacta
    67021 tgtgcgtccg ccaacgccgc tggacagaga agcgcgtaac cgtggcacgt cggtgtactt
    67081 cccttcgcag gttatcccga tgctgccgga agtgctctct aacggcctgt gttcgctcaa
    67141 cccgcaggta gaccgcctgt gtatggtgtg cgagatgacg gtttcgtcga aaggccgcct
    67201 gacgggctac aaattctacg aagcggtgat gagctctcac gcgcgtctga cctacaccaa
    67261 agtctggcat attctgcagg gcgatcagga tctgcgcgag cagtacgccc cgctggttaa
    67321 gcatctcgaa gagttgcata acctctataa agtgctggat aaagcccgtg aagaacgcgg
    67381 tgggatctca tttgagagcg aagaagcgaa gttcattttc aacgctgaac gccgtattga
    67441 acgtatcgaa cagacccagc gtaacgacgc gcacaaatta attgaagagt gcatgattct
    67501 ggcgaatatc tcggcggcgc gtttcgttga gaaagccaaa gaaccggcac tgttccgtat
    67561 tcacgacaag ccgagcaccg aagcgattac ctctttccgt tcagtgctgg cggagctggg
    67621 gctggagctg ccgggtggta acaagccgga accgcgtgac tacgcggaac tgctggagtc
    67681 ggttgccgac cgtcctgatg cagaaatgct gcaaaccatg ctgctacgct cgatgaaaca
    67741 ggcgatttac gatccagaaa accgtggtca cttcggtctg gcattgcagt cctatgcgca
    67801 ctttacttca ccgattcgtc gttatcctga cctgacgctg caccgcgcca ttaaatatct
    67861 gctggcgaaa gagcaggggc atcagggcaa caccactgaa accggcggct accattattc
    67921 gatggaagag atgttgcaac tgggtcagca ctgttcgatg gcggaacgtc gtgccgacga
    67981 agcaacgcgc gatgtcgctg actggctgaa gtgtgacttc atgctcgacc aggtaggtaa
    68041 cgtctttaaa ggcgtaattt ccagcgtcac tggctttggc ttcttcgtcc gtctggacga
    68101 cttgttcatt gatggtctgg tccatgtctc ttcgctggac aatgactact atcgctttga
    68161 ccaggtaggg caacgcctga tgggggaatc cagcggccag acttatcgcc tgggcgatcg
    68221 cgtggaagtt cgcgtcgaag cggttaatat ggacgagcgc aaaatcgact ttagtctgat
    68281 ctccagtgaa cgcgcaccgc gcaacgtcgg taaaacggcg cgcgagaaag cgaaaaaagg
    68341 cgatgcaggc aaaaaaggcg gcaagcgtcg tcaggtcggt aaaaaggtaa actttgagcc
    68401 agacagcgcc ttccgcggtg agaaaaaaac gaagccgaaa gcggcgaaga aagacgcgag
    68461 aaaagcgaaa aagccatcgg cgaaaacgca gaaaatagcc gcagcgacca aagcgaagcg
    68521 tgcggcgaag aaaaaagtgg cagagtgatc aataccctct ttaaaagaag agggttagat
    68581 tgctgacaaa atgcgctttg ttcatgccgg atgcggcgtg aacgccttat ccggcctaca
    68641 taatcacgca aattcaatat attgcagaga tcatgtaggc cggataaacg tagtgcatca
    68701 ggcaaaacgt aaacaacgag tacattaatg agcgaaatga tttacggcat ccacgcagtg
    68761 caggccctgc tggagcgcgc ccctgaacgt tttcaggaag tctttatttt aaaaggccgt
    68821 gaagataaac gtctgttacc gctgattcac gcccttgagt ctcagggcgt ggttatccag
    68881 ttggcaaacc gccaatatct cgacgagaaa agcgacggtg ccgtgcatca gggcattatc
    68941 gcccgcgtga agccaggacg tcagtatcag gaaaacgatc tgccggatct gatcgcttcg
    69001 ctcgatcaac cgttcctgct gatcctcgac ggtgtaaccg atccgcacaa cctcggcgcg
    69061 tgcctgcgta gcgcggacgc tgctggcgtt cacgcggtga ttgtgccgaa agatcgctcc
    69121 gcacagctca acgctacggc gaaaaaagta gcctgcggcg cggcagaaag cgttccactg
    69181 attcgggtga ctaaccttgc gcgcaccatg cgtatgttgc aggaagagaa tatctggatc
    69241 gtcggtacgg caggcgaggc ggatcataca ctctatcaga gcaaaatgac cggacgcctg
    69301 gcgctggtga tgggcgcgga aggtgaaggt atgcgtcgcc tgacccgtga acattgcgat
    69361 gagttgatca gcatcccaat ggcaggaagt gtttcttccc tgaacgtttc ggttgcaacc
    69421 ggaatttgct tatttgaagc ggttcgtcag cgcagctagt tcgtctgaat tatgaaaagc
    69481 catccagatt tggatggttt tttt
//
LOCUS       DAFNBJ010000005        65375 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000005 DAFNBJ010000000
VERSION     DAFNBJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.005"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..2413
                     /locus_tag="L2X49_000330"
     CDS             <1..2413
                     /locus_tag="L2X49_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBN4795477.1"
                     /translation="TTANAEEYYFDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVD
                     IWLNKKKVSQKKITFTANAEQLLQPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLE
                     QIIPGTAAEFDFNHQRLNLSIPQIALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNR
                     YRQGNRSQRQYLNMQNGANFGPWRLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQL
                     LLGESATSGSIFSSYTFTGVQLASDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYV
                     IYQSNVPAGAFEINDLYPSSNSGDLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYS
                     ATAGRYRADANSDSKEPEFAEATAIYGLNNTFTLYSGLLGSEDYYALGIGIGGTLGAL
                     GALSMDINRADTQFDNQHSFHGYQWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEA
                     NTRNWDYNSRQKSEIQFNISQTIFDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWG
                     IGYSLNYQYSRYTDQNNDRALSLNLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDG
                     SLLDDGRLSYSLEQSLDDDNNHNSSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGV
                     VIHPHGVTLSQYLGNAFALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLS
                     VDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALAS
                     NDETGQQSIVDEGGILYLSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQG
                     TAQCH"
     gene            2424..3515
                     /locus_tag="L2X49_000331"
     CDS             2424..3515
                     /locus_tag="L2X49_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4795478.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLKANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(3558..4418)
                     /gene="rsmI"
                     /locus_tag="L2X49_000332"
     CDS             complement(3558..4418)
                     /gene="rsmI"
                     /locus_tag="L2X49_000332"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBN4795479.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            4483..6519
                     /gene="lpoA"
                     /locus_tag="L2X49_000333"
     CDS             4483..6519
                     /gene="lpoA"
                     /locus_tag="L2X49_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBN4795480.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            6477..6872
                     /locus_tag="L2X49_000334"
     CDS             6477..6872
                     /locus_tag="L2X49_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBN4795481.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6892..7482
                     /gene="diaA"
                     /locus_tag="L2X49_000335"
     CDS             6892..7482
                     /gene="diaA"
                     /locus_tag="L2X49_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBN4795482.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            7492..8067
                     /gene="yraP"
                     /locus_tag="L2X49_000336"
     CDS             7492..8067
                     /gene="yraP"
                     /locus_tag="L2X49_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBN4795483.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(8272..9312)
                     /locus_tag="L2X49_000337"
     CDS             complement(8272..9312)
                     /locus_tag="L2X49_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBN4795484.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLSWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGTVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(9385..10020)
                     /locus_tag="L2X49_000338"
     CDS             complement(9385..10020)
                     /locus_tag="L2X49_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBN4795485.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            10148..10666
                     /gene="yhbO"
                     /locus_tag="L2X49_000339"
     CDS             10148..10666
                     /gene="yhbO"
                     /locus_tag="L2X49_000339"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBN4795486.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(10646..11089)
                     /locus_tag="L2X49_000340"
     CDS             complement(10646..11089)
                     /locus_tag="L2X49_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBN4795487.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            11140..11442
                     /gene="yhbQ"
                     /locus_tag="L2X49_000341"
     CDS             11140..11442
                     /gene="yhbQ"
                     /locus_tag="L2X49_000341"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBN4795488.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(11429..11932)
                     /locus_tag="L2X49_000342"
     CDS             complement(11429..11932)
                     /locus_tag="L2X49_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBN4795489.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11926..12450)
                     /locus_tag="L2X49_000343"
     CDS             complement(11926..12450)
                     /locus_tag="L2X49_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBN4795490.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     NAPETKQTSVGEPC"
     gene            12659..13654
                     /locus_tag="L2X49_000344"
     CDS             12659..13654
                     /locus_tag="L2X49_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBN4795491.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            13663..14541
                     /locus_tag="L2X49_000345"
     CDS             13663..14541
                     /locus_tag="L2X49_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBN4795492.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            14747..15754
                     /locus_tag="L2X49_000346"
     CDS             14747..15754
                     /locus_tag="L2X49_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBN4795493.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15872..17116)
                     /gene="mtr"
                     /locus_tag="L2X49_000347"
     CDS             complement(15872..17116)
                     /gene="mtr"
                     /locus_tag="L2X49_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBN4795494.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(17270..19159)
                     /gene="deaD"
                     /locus_tag="L2X49_000348"
     CDS             complement(17270..19159)
                     /gene="deaD"
                     /locus_tag="L2X49_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBN4795495.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(19152..19232)
                     /gene="yrbN"
                     /locus_tag="L2X49_000349"
     CDS             complement(19152..19232)
                     /gene="yrbN"
                     /locus_tag="L2X49_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBN4795496.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(19339..20223)
                     /gene="nlpI"
                     /locus_tag="L2X49_000350"
     CDS             complement(19339..20223)
                     /gene="nlpI"
                     /locus_tag="L2X49_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBN4795497.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(20332..22467)
                     /gene="pnp"
                     /locus_tag="L2X49_000351"
     CDS             complement(20332..22467)
                     /gene="pnp"
                     /locus_tag="L2X49_000351"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBN4795498.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(22714..22983)
                     /gene="rpsO"
                     /locus_tag="L2X49_000352"
     CDS             complement(22714..22983)
                     /gene="rpsO"
                     /locus_tag="L2X49_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBN4795499.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(23132..24076)
                     /gene="truB"
                     /locus_tag="L2X49_000353"
     CDS             complement(23132..24076)
                     /gene="truB"
                     /locus_tag="L2X49_000353"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBN4795500.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(24076..24477)
                     /gene="rbfA"
                     /locus_tag="L2X49_000354"
     CDS             complement(24076..24477)
                     /gene="rbfA"
                     /locus_tag="L2X49_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBN4795501.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(24641..27313)
                     /gene="infB"
                     /locus_tag="L2X49_000355"
     CDS             complement(24641..27313)
                     /gene="infB"
                     /locus_tag="L2X49_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBN4795502.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(27338..28825)
                     /gene="nusA"
                     /locus_tag="L2X49_000356"
     CDS             complement(27338..28825)
                     /gene="nusA"
                     /locus_tag="L2X49_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBN4795503.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(28853..29305)
                     /gene="rimP"
                     /locus_tag="L2X49_000357"
     CDS             complement(28853..29305)
                     /gene="rimP"
                     /locus_tag="L2X49_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBN4795504.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(29512..29588)
                     /locus_tag="L2X49_000358"
     tRNA            complement(29512..29588)
                     /locus_tag="L2X49_000358"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29552..29554),aa:Met,seq:cat)
     gene            29936..31279
                     /gene="argG"
                     /locus_tag="L2X49_000359"
     CDS             29936..31279
                     /gene="argG"
                     /locus_tag="L2X49_000359"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBN4795505.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(31287..32912)
                     /locus_tag="L2X49_000360"
                     /pseudo
     CDS             complement(31287..32912)
                     /locus_tag="L2X49_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(33371..33457)
                     /locus_tag="L2X49_000361"
     tRNA            complement(33371..33457)
                     /locus_tag="L2X49_000361"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33421..33423),aa:Leu,seq:gag)
     gene            complement(33472..33804)
                     /gene="secG"
                     /locus_tag="L2X49_000362"
     CDS             complement(33472..33804)
                     /gene="secG"
                     /locus_tag="L2X49_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBN4795506.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(34032..35369)
                     /gene="glmM"
                     /locus_tag="L2X49_000363"
     CDS             complement(34032..35369)
                     /gene="glmM"
                     /locus_tag="L2X49_000363"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBN4795507.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(35362..36210)
                     /gene="folP"
                     /locus_tag="L2X49_000364"
     CDS             complement(35362..36210)
                     /gene="folP"
                     /locus_tag="L2X49_000364"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBN4795508.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(36300..38234)
                     /gene="ftsH"
                     /locus_tag="L2X49_000365"
     CDS             complement(36300..38234)
                     /gene="ftsH"
                     /locus_tag="L2X49_000365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBN4795509.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(38334..38963)
                     /gene="rlmE"
                     /locus_tag="L2X49_000366"
     CDS             complement(38334..38963)
                     /gene="rlmE"
                     /locus_tag="L2X49_000366"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBN4795510.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLAELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            39089..39382
                     /gene="yhbY"
                     /locus_tag="L2X49_000367"
     CDS             39089..39382
                     /gene="yhbY"
                     /locus_tag="L2X49_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBN4795511.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(39538..40014)
                     /gene="greA"
                     /locus_tag="L2X49_000368"
     CDS             complement(39538..40014)
                     /gene="greA"
                     /locus_tag="L2X49_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBN4795512.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            40262..41413
                     /gene="dacB"
                     /locus_tag="L2X49_000369"
     CDS             40262..41413
                     /gene="dacB"
                     /locus_tag="L2X49_000369"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBN4795513.1"
                     /translation="MRFSRFIIGLTSCIAFSIQAANVDEYVTQLPAGVNQIDGNVLID
                     TSIFASHDKAPGWPWNDMTQCFSAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYY
                     PVTMFSQVRTLPRGSAEAQYCELDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASY
                     AGAILKDELKQAGITWSGTLLRQTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIAD
                     TVFRMIGHARFNVPGTWRAGSDAVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATM
                     MQVLQYIAQHDNELNFISMLPLAGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLA
                     GFITTASGQRMAFVQYLSGYAVEPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(41599..42771)
                     /gene="cgtA"
                     /locus_tag="L2X49_000370"
     CDS             complement(41599..42771)
                     /gene="cgtA"
                     /locus_tag="L2X49_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBN4795514.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(42787..43752)
                     /locus_tag="L2X49_000371"
     CDS             complement(42787..43752)
                     /locus_tag="L2X49_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBN4795515.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(43879..44136)
                     /gene="rpmA"
                     /locus_tag="L2X49_000372"
     CDS             complement(43879..44136)
                     /gene="rpmA"
                     /locus_tag="L2X49_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBN4795516.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(44157..44468)
                     /gene="rplU"
                     /locus_tag="L2X49_000373"
     CDS             complement(44157..44468)
                     /gene="rplU"
                     /locus_tag="L2X49_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBN4795517.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            44727..45698
                     /gene="ispB"
                     /locus_tag="L2X49_000374"
     CDS             44727..45698
                     /gene="ispB"
                     /locus_tag="L2X49_000374"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBN4795518.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            45925..46203
                     /gene="sfsB"
                     /locus_tag="L2X49_000375"
     CDS             45925..46203
                     /gene="sfsB"
                     /locus_tag="L2X49_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBN4795519.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(46251..47510)
                     /gene="murA"
                     /locus_tag="L2X49_000376"
     CDS             complement(46251..47510)
                     /gene="murA"
                     /locus_tag="L2X49_000376"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBN4795520.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(47565..47819)
                     /gene="ibaG"
                     /locus_tag="L2X49_000377"
     CDS             complement(47565..47819)
                     /gene="ibaG"
                     /locus_tag="L2X49_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBN4795521.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(47979..48272)
                     /gene="mlaB"
                     /locus_tag="L2X49_000378"
     CDS             complement(47979..48272)
                     /gene="mlaB"
                     /locus_tag="L2X49_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBN4795522.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGIICIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(48272..48907)
                     /gene="mlaC"
                     /locus_tag="L2X49_000379"
     CDS             complement(48272..48907)
                     /gene="mlaC"
                     /locus_tag="L2X49_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBN4795523.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(48926..49477)
                     /gene="mlaD"
                     /locus_tag="L2X49_000380"
     CDS             complement(48926..49477)
                     /gene="mlaD"
                     /locus_tag="L2X49_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBN4795524.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(49482..50264)
                     /gene="mlaE"
                     /locus_tag="L2X49_000381"
     CDS             complement(49482..50264)
                     /gene="mlaE"
                     /locus_tag="L2X49_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBN4795525.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(50272..51081)
                     /gene="mlaF"
                     /locus_tag="L2X49_000382"
     CDS             complement(50272..51081)
                     /gene="mlaF"
                     /locus_tag="L2X49_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBN4795526.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            51291..52268
                     /locus_tag="L2X49_000383"
     CDS             51291..52268
                     /locus_tag="L2X49_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBN4795527.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            52282..53268
                     /gene="kdsD"
                     /locus_tag="L2X49_000384"
     CDS             52282..53268
                     /gene="kdsD"
                     /locus_tag="L2X49_000384"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBN4795528.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            53289..53855
                     /gene="kdsC"
                     /locus_tag="L2X49_000385"
     CDS             53289..53855
                     /gene="kdsC"
                     /locus_tag="L2X49_000385"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBN4795529.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            53852..54427
                     /gene="lptC"
                     /locus_tag="L2X49_000386"
     CDS             53852..54427
                     /gene="lptC"
                     /locus_tag="L2X49_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBN4795530.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            54396..54953
                     /gene="lptA"
                     /locus_tag="L2X49_000387"
     CDS             54396..54953
                     /gene="lptA"
                     /locus_tag="L2X49_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBN4795531.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            54960..55685
                     /gene="lptB"
                     /locus_tag="L2X49_000388"
     CDS             54960..55685
                     /gene="lptB"
                     /locus_tag="L2X49_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBN4795532.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            55733..57166
                     /gene="rpoN"
                     /locus_tag="L2X49_000389"
     CDS             55733..57166
                     /gene="rpoN"
                     /locus_tag="L2X49_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBN4795533.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            57189..57476
                     /gene="hpf"
                     /locus_tag="L2X49_000390"
     CDS             57189..57476
                     /gene="hpf"
                     /locus_tag="L2X49_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBN4795534.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            57594..58085
                     /gene="ptsN"
                     /locus_tag="L2X49_000391"
     CDS             57594..58085
                     /gene="ptsN"
                     /locus_tag="L2X49_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBN4795535.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            58131..58985
                     /gene="rapZ"
                     /locus_tag="L2X49_000392"
     CDS             58131..58985
                     /gene="rapZ"
                     /locus_tag="L2X49_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBN4795536.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            58982..59254
                     /gene="npr"
                     /locus_tag="L2X49_000393"
     CDS             58982..59254
                     /gene="npr"
                     /locus_tag="L2X49_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBN4795537.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            59468..60100
                     /gene="yrbL"
                     /locus_tag="L2X49_000394"
     CDS             59468..60100
                     /gene="yrbL"
                     /locus_tag="L2X49_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBN4795538.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(60097..60825)
                     /gene="mtgA"
                     /locus_tag="L2X49_000395"
     CDS             complement(60097..60825)
                     /gene="mtgA"
                     /locus_tag="L2X49_000395"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBN4795539.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(60822..61475)
                     /gene="elbB"
                     /locus_tag="L2X49_000396"
     CDS             complement(60822..61475)
                     /gene="elbB"
                     /locus_tag="L2X49_000396"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBN4795540.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(61705..64041)
                     /gene="arcB"
                     /locus_tag="L2X49_000397"
     CDS             complement(61705..64041)
                     /gene="arcB"
                     /locus_tag="L2X49_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBN4795541.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(64137..65066)
                     /locus_tag="L2X49_000398"
     CDS             complement(64137..65066)
                     /locus_tag="L2X49_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBN4795542.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARLRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
BASE COUNT        16136 a        17670 c        15992 g        15577 t
ORIGIN      
        1 cactaccgct aacgccgaag agtattattt cgaccccatt atgctggaaa ccacaaaaag
       61 tggtatgcaa acaaccgatc tgtcacgatt ttcaaagaaa tacgcacaac taccaggaac
      121 ttatcaggtt gatatctggc tgaataaaaa gaaggtttca cagaaaaaaa ttacatttac
      181 cgccaatgcc gagcaacttc tgcagccaca gtttacggtt gaacaactac gtgagctggg
      241 tattaaggtg gatgaaatcc cggcgctggc tgaaaaagat gacgatagcg tgatcaactc
      301 gcttgaacaa atcattcccg gtacagctgc tgaatttgat ttcaatcatc agcgacttaa
      361 tttgagcatt ccccaaattg cactgtaccg tgatgcaaga ggttacgtct ccccttctcg
      421 ttgggacgat ggtataccaa cgctgtttac caactactcg tttacaggtt ctgataaccg
      481 ttaccgccag ggcaatcgta gccaacgaca gtacctgaat atgcaaaatg gtgctaattt
      541 tggcccctgg cgattacgca actattccac atggacacgc aacgatcaga cgtcaagctg
      601 gaacactatc agtagttatt tacaacgtga tatcaaggcg ttgaaatctc agttgcttct
      661 gggagaaagc gccaccagcg gcagtatttt ttccagctac acctttactg gcgtgcaact
      721 cgcttccgac gataatatgt tgccaaacag ccagcgcgga tttgccccaa cggtacgcgg
      781 tatcgcaaac agtagtgcaa tcgtgactat caggcaaaat ggttatgtga tctatcaaag
      841 caacgtgcca gcgggtgcct ttgaaattaa cgatctctac ccctcttcca acagcggcga
      901 tttagaagtc acgattgaag aaagtgacgg tacgcaacgt cgctttatcc agccttattc
      961 ttcattaccc atgatgcagc gacctgggca tctaaaatat agcgcgaccg ctggacgcta
     1021 tcgcgctgat gcaaacagtg atagcaagga acccgaattt gctgaagcca cggcaatata
     1081 tggtttgaat aatactttta cgctgtatag tggcctgctc ggttctgaag attattatgc
     1141 gctggggatc ggtatcggcg gcacacttgg cgcactgggc gcgttgtcga tggatatcaa
     1201 cagagctgac acccaattcg ataaccagca ctcttttcat ggctatcaat ggcgtacgca
     1261 gtacatcaaa gatatcccgg aaaccaacac caatatcgct gtcagctact atcgctatac
     1321 caacgatggc tattttagtt ttgatgaagc caatacccgc aattgggact ataacagtcg
     1381 ccaaaaaagt gaaattcaat tcaacatcag ccagacaata tttgatgggg taagtctgta
     1441 tgcctccggt tcacagcaag actattgggg caataacgag aaaaacagga atatctctgt
     1501 tggggtttcc ggccagcaat ggggaattgg ttacagcctg aattatcaat acagccgcta
     1561 cactgatcaa aataatgacc gtgcactctc tttgaatctc agtattccgt tagaacgctg
     1621 gttaccgcgt agccgggttt cctatcagat gaccagccag aaagatcgcc caacccaaca
     1681 tgaaatgcgt cttgatggtt cactgctgga tgatggtcgc ctgagctata gcctggaaca
     1741 aagtctggat gacgataaca accataacag tagcgtgaac gccagttacc gttcacctta
     1801 tggaaccttc agtgccggat acagttacgg taatgacagt agccaataca attacggcgt
     1861 taccggcggc gtggttatcc atcctcatgg tgtgacgctc tcgcaatatc tgggcaacgc
     1921 ttttgcgctt attgatgcta acggggcatc tggcgtgagg atacaaaact atccggggat
     1981 tgctactgat ccctttggct atgcagtggt tccttatctc acgacttatc aggaaaaccg
     2041 tctctcggta gatactacgc agctgcccga taacgtcgat cttgagcaaa caacacagtt
     2101 tgtggtgccc aacagaggtg caatggtagc ggcgcgtttc aacgccaata tcggttatcg
     2161 cgtacttgtt acagtcagcg atcgcaacgg taaaccgttg ccctttggcg ctcttgccag
     2221 caacgatgag acggggcaac aaagtatcgt cgatgagggc ggcatactat atctctctgg
     2281 gatatcgagt aaatcacaaa gctggactgt acgctgggga aatcaggcag atcaacaatg
     2341 tcagtttgct tttagtacac cggattcaga accaacaacc tctgtattac aaggcacagc
     2401 gcagtgccat taaggataaa aaaatgaaaa gagcgcctct tataacagga cttttgttga
     2461 tatccacatc ctgcgcttat gcctcctcag gagggtgtgg agccgacagc actagcggtg
     2521 cgacaaatta cagcagtgtg gttgatgatg ttacggtgaa ccagacagat aacgtgacag
     2581 gacgggagtt tacctctgca acgctaagta gcactaactg gcaatacgcc tgttcctgct
     2641 ctgcgggtaa ggcagttaaa cttgtctata tggtcagccc tgtactcacc accactggac
     2701 atcaggcagg atattacaaa ctcaatgata gcctggatat taaaaccacc ttaaaggcga
     2761 atgacattcc aggactcgtg actgaccaaa ccgtttctgt taacacccga ttcacacaga
     2821 taaaaagcaa caccgtatat tctgctgcaa cccaaacggg tgtttgccag ggtgacacgt
     2881 ctcgttatgg acccgttaat attggtgcaa ataccacctt taccctgtat gtcaccaagc
     2941 catttctcgg ctcgatgacc attccgaaaa cggatattgc cgtcattaaa ggcgcgtggg
     3001 tcgatggaat gggaagcccg tctacaggtg acttccatga tttagtcaag ttatcgattc
     3061 aaggaaatct caccgccccg cagtcgtgca aaattaatca gggcgatgtt attaaggtta
     3121 attttggatt catcaatggt cagaagttta ccacccgcaa tgccatgcca gacggtttta
     3181 ctccagtaga ctttgatatc acttatgact gtggtgatac ttcaaagatt aaaaactcgt
     3241 tgcaaatgcg catcgacggt acaactgggg tagtagacca gtacaacctt gtcgccagac
     3301 gaagaagttc agacaatgcg cccgatgtcg gtattcgtat tgaaaatctc ggcggcggag
     3361 ttgcaaatat tccttttcag aacggtatcc ttcccgttga tccttccggg catggcaccg
     3421 tcaatatgcg cgcctggcca gttaatctgg tcggtggtga gctggaaaca ggaaaatttc
     3481 agggcacagc caccattacc gtcatcgtgc ggtaaaagaa gacgttgcgt tttgattaac
     3541 gcaacgcttc ggcactctta cccctgctgc tccagcgcat acttatacag cgcatttttc
     3601 ttcacgccat gaatctctgc ggccagcgcc gccgcttttt tcagcggtag ttctgcctgt
     3661 agcagcgcca gcgtgcgcag ggcatcggcg ggtaagtctt cttcctgcgc tttatggcct
     3721 tcgacaatca gcaccatttc gcctttgcga cggttttcat cttctttgac ccacgccagc
     3781 agctcgccaa cgggcgcgcc gtgaatggtt tcccaggttt tggtgagctc acgcgccaga
     3841 accacgtagc gggattcgcc taataccgca acgatatctt ccaggctgtc taacagacgg
     3901 tgggttgatt cataaaaaat cagagtgcgc ggctccgctt caatggcttt tagcgcatca
     3961 cggcggcctt ttgatttggc aggtaaaaag ccttcgtaac agaaacggtc agagggtaaa
     4021 cccgcggcgc ttaacgcagt gatagcagca cacggcccgg gtagcggcac cacgcgaatc
     4081 cccgcttcac ggcaggtacg caccagatgg tagccaggat cgttaattag cggcgttccg
     4141 gcatcggaaa ccagcgcaat gttttgcccc tcttgcagct tcgccagcag cgtttcggct
     4201 ttttgttgtt cgttatggtc gtgcagcgca aacaaccggg cattaatccc aaaatgttgc
     4261 agcaataaac cggtgtgacg agtatcctcg gcggcaatca gatcaacggc ctgtaatacc
     4321 tctaacgcac gctgggtgat atccgccaga ttgccgattg gcgtcggtac aatgtaaagc
     4381 tggccctgag aattatccgc cgattggtgt tgtttcattg tgtcgtccgt attgccgatt
     4441 taatattgag cattgcgtaa aaaaaatatc actggataca ttatggtacc ctcaacattt
     4501 tctcgtttga aagccgcgcg ttgtctgcct gttgttctgg cagccctgat tttcgccggt
     4561 tgtggcaccc atactcccga tcagtccact gcttatatgc agggcacggc gcaggctgat
     4621 tctgcctttt atcttcagca gatgcagcaa agctctgatg ataccaggat caactggcaa
     4681 ttactcgcca ttcgtgcact ggtgaaagaa ggtaaaaccg ggcaggcggt ggagttgttt
     4741 aaccaactac cgcaagaact gaacgattct cagcgtcgcg agaaaacact gctggcggta
     4801 gagattaaac tggcgcagaa agattttgct ggcgcgcaaa atttgctggc gaaaatcaca
     4861 cctgccgatt tagaacaaaa ccagcaagcc cgttactggc aggcaaaaat cgatgccagc
     4921 caggggcgtc cttccattga tttactgcgc gcgttaattg ctcaggaacc gctgctcggc
     4981 gcgaaagaaa aacagcagaa tattgatgcc acctggcagg cgctctcctc catgactcag
     5041 gaacaggcga atacgctggt gatcaacgcc gacgaaaata ttctgcaagg ctggctggat
     5101 ctgcagcgcg tctggtttga taaccgaaac gatcccgaca tgatgaaagc cgggatcgcc
     5161 gactggcaga aacgttatcc gaacaatccg ggcgcgaaaa tgctgccaac gcagttggtt
     5221 aacgtaaaag cgtttaaacc agcatcaacc aacaaaatcg ccctgctgtt gccgctgaat
     5281 ggccaggcag cggtatttgg tcgcactatt cagcaaggct ttgaagcggc gaaaaatatc
     5341 ggcactcagc cagtggcggc tcaggtagct gccgcacctg ccgcagacgt agcagaacaa
     5401 cctcagccgc aaaccgtgga tggcgttgcc agcccggcac aagcctcggt tagcgatctg
     5461 accggtgaac agcctgcagc ccagccggtg cctgtaagcg ccccggcgac aagcaccgca
     5521 gcggtaagcg cacccgcaaa tccatccgca gagctgaaaa tctacgatac ctcatcacaa
     5581 ccacttagcc agatcttaag ccaggttcag caggatggcg cgagtattgt ggtcggtccg
     5641 ttgctgaaaa ataacgttga agagttgctg aagagcaaca ccccgctgaa cgtgctggca
     5701 ctgaaccagc cggagaatat cgaaaaccgc gtcaatattt gttacttcgc gctttcacct
     5761 gaagacgaag cgcgcgatgc agcgcgtcat attcgtgacc agggtaaaca agcgccgctg
     5821 gtgctgatcc cacgcagttc attgggcgat cgcgtagcca atgcgtttgc gcaagagtgg
     5881 cagaaactgg gcggcggcac cgttctgcaa caaaaatttg gttccaccag cgaattacgc
     5941 gcgggtgtta acggcggttc tggtattgct ttaacgggta gcccgattac tcccagagcg
     6001 acaaccgact ccggcatgac gaccaacaat ccaacgctgc aaaccacgcc aaccgatgac
     6061 cagttcacca ataatggcgg tcgtgtcgat gcggtgtaca ttgtggcaac gccgggtgaa
     6121 atcgctttta tcaaaccgat gatcgccatg cgtaacggta gccagagcgg tgcaacgctg
     6181 tacgccagct cccgcagtgc gcaagggacc gctggcccgg atttccgact ggagatggaa
     6241 ggcttgcagt acagcgaaat cccgatgctg gcaggcggta atctgccgtt aatgcagcag
     6301 gcgctcagcg cggtgaacaa cgattattcg ctggctcgca tgtatgcgat gggcgttgat
     6361 gcctggtcgt tggcaaatca tttctctcaa atgcgccagg ttcagggttt tgaaatcaac
     6421 ggtaataccg gaagcctgac ggcaaacccg gattgcgtga ttaacaggaa gttatcatgg
     6481 ctacagtacc aacaaggtca ggtagtcccc gccagttaac caccaaacag accggcgatg
     6541 cgtgggaagc acaagcgcgt cgctggctgg aaggcaaagg actgcggttt atcgccgcta
     6601 acgtgaacga gcgtggcggc gagatcgatc tgataatgcg tgaaggccgg accaccgttt
     6661 ttgtcgaggt acgctatcgc cgctctgcgc tttatggcgg cgcggcagcc agtgtgaccc
     6721 gcagcaaaca acacaaatta ttacagactg cccgcttgtg gctcgcgcgt cataatggga
     6781 gttttgatac tgtggattgc cggttcgatg tggtagcctt caccgggaat gaggttgagt
     6841 ggattaagga tgcctttaat gaccactcat aattaaggtt taaggattag cgtgcaagaa
     6901 agaattaaag cttgcttcac tgaaagcatt caaactcaaa ttgcggcggc agaggcgctt
     6961 ccggatgcca tctcccgtgc agccatgacg ctggttcagt ctctgctcaa tggcaacaaa
     7021 atcctctgtt gtggtaatgg aacttccgct gccaatgcac agcattttgc tgccagcatg
     7081 atcaaccgtt tcgaaacgga gcggcccagc ttacctgcca ttgcactaaa tactgataat
     7141 gttgtcttaa cggcgattgc caacgatcgc ttacatgatg aagtgtatgc aaaacaggtg
     7201 cgggcgctgg gtcatgcggg agatgtattg ttagccattt ccacgcgtgg caacagccgc
     7261 gatattgtaa aagcggtaga agctgccgtt acgcgtgata tgaccattgt ggcattaacc
     7321 ggctatgacg gcggtgaact tgcaggtttg ttagggccac aggatgtgga aatccgcatt
     7381 ccttcgcatc gtagtgctcg cattcaggaa atgcatatgc tgacggtaaa ttgcctgtgc
     7441 gatctgatcg ataacacgct tttccctcac caggatgatt aaggagaata catgaaggca
     7501 ttatcgccaa tcgcagtcct tatttccgcg ctgctgttgc aaggttgtgt tgccgctgcc
     7561 gtagtgggta ccgctgctgt gggtaccaaa gccgcaactg acccacgcag tgtcggcacc
     7621 caggtggacg atggtaccct ggaagtgcgc gtgaacagcg cattgtcgaa agacgaacag
     7681 attaagaaag aagcgcgcat taatgtaacg gcctatcagg gcaaagtgct gctggttggg
     7741 cagtcaccaa acgctgaact ttcggctcgc gccaaacaga ttgctatggg cgtagacggt
     7801 gccaacgaag tgtataacga gattcgtcag ggccaaccga ttggtctggg cgaagcgtct
     7861 aacgatacgt ggatcaccac caaagtgcgt tcgcagctct taaccagcga cctggtgaaa
     7921 tcgtccaacg tgaaagtgac caccgaaaac ggtgaagtgt tcctgatggg gctggtgact
     7981 gaacgtgaag cgaaagcggc ggcagatatt gccagccggg taagcggcgt gaaacgggta
     8041 actacggcgt ttacgtttat taaatagcag ccccttgtaa tgcctgatgc gacgcttgcc
     8101 gcgtcttatc aggcctacaa cgacacaaaa cgtaggtcag ataaggtgtt tacgccgcat
     8161 ccgactgctg ttgcctgatg cgacgcttgc cgcgtcttat caggcctaca acgatacaaa
     8221 ccgtaggtca gataaggcgt ttacgccgca tccgacatgt tttccctcaa atcagaacaa
     8281 cagcgccagc ccgccgacaa tcacaccaga cactgccacc atcgcccccg tcagccataa
     8341 ggctttcgcc gggaacgctt tgcgcagcat aatcagtgac ggcaaactca ccgccgggag
     8401 cgtcatcaac aatgccagcg ccggagcagt tcccatacct gccagcatca tcgtttgtac
     8461 aatcggaatt tctgctgccg tgggaatgac aaacaagcat cctgccaccg ccatcgccac
     8521 cacccacatc aggctgttat cgacagtacc atcggcatgg gggaataacc agacgcgagc
     8581 ggcacccaac accagtactg caaggatgta aaccgggatc gtactccaga aaagcgtcca
     8641 tagcgccctg ccccagcggc taaaaaaacc gccctgtgct tccggtatgt caatttcgac
     8701 cggtgcctgc gtttgcggtg tttcacgcac ccatttttgc accagcgtcg caatcagcaa
     8761 caccatcacc agcccggcca ccagacgaat cgccgcaaaa ccccagctga ggacaaagcc
     8821 cataaacacc agcgtcgccg ggtttaacac cggattgccc atccagaatg ccagcgcacc
     8881 gcccatcgac acctgttggc gacgcattcc cgccgcgacc ggagccgcac agcaggtaca
     8941 catcatgccc ggcaacgaaa acagcgttcc cagcagcgtg ccgcgaaagc gcgattgccc
     9001 aagcgtacgc aacaaccagt cacgcgggat cagcacctga atcaacgaac cgaggatcac
     9061 ccccagcacc gccgctttcc ataccgcgag gaaatagatc atcgcgtaat ccaacgccgc
     9121 ctgccatggg ttagcatccg cctgcgcaag gatagattta ccgatactgt gggtttcggc
     9181 agcagtaaac gctttgccgt agtaaggttc ccatttcaca taccagagac cggcaatgac
     9241 aacgagaaag aaaagcgcgg gtttccacca ctgaatgggc gttgccgcct gagatgaaga
     9301 ctgaccagtc atagcattcc ccaggaataa gttatgataa ttgagcgcgt gaatattacg
     9361 ctcactatca attcttggga ataattattc agctcttttg cgtaattctg aagagcttaa
     9421 aatcgtcacg ccttcatgct caggccgcat cgactctgcc agcatgaccc gcgccacgtc
     9481 tctcgcatca atggatttcc agttacctgg taacaaacgg aacagcggcg caaaaagcgt
     9541 ttcgttcatc cgctgtttgc tacgatcgcc cagtaacatc gacgggcgag caatggtcaa
     9601 tttcggccag ttctgggcaa ttaatgcttc ttccatctcc cctttgacgc ggttatagaa
     9661 aaacggcgag tgggcattgg cacccatagc actgaccacc agcatatgct gcgcgcccag
     9721 ccgccgcccg gttaatgcgg tatccactac cagcgtgtaa tcggcatgaa taaacgcctc
     9781 tttgctcccc gcttctcgcc gcgtggtgcc gagacaacaa aacacaatgt cgatgggatc
     9841 ggtgacctgc gccagcgcgt cagtcagttg cggatcatgg ggattgaaca ctcctggcat
     9901 atctcccaac ggacgtcgcg tcggtgcagc aatagcgtta actttcggtt cgttaatcaa
     9961 catccgcagc aggtgaccgc ccaccagccc cgttgcgcct gtaatcagta cctgactcat
    10021 cttcgctcct ttacagaatt gtctgccttg cgctccccgg ctcatgcacc aggcttaata
    10081 gaccatgagg taattatccc cgattgtgag aaattcgcct catccaatgc aacaacgcgg
    10141 aggaagcatg agtaagaaaa ttgccgtttt aatcactgat gaatttgagg attcagaatt
    10201 tacttcaccc gcagacgaat tccgtaaagc cggacacgaa gtgattacca ttgaaaaaca
    10261 agcgggtaaa acggtgaaag gcaaaaaagg agaagccagc gtgaccatcg ataaatccat
    10321 cgatgaagtg acgccagcgg agtttgatgc cctgttgcta ccgggcggtc attcaccgga
    10381 ttatctgcgt ggcgacaacc gttttgtcac ctttacccgt gattttgtga atagcggcaa
    10441 acctgttttc gccatctgtc acggtccgca gttgctgatc agcgccgatg tgattcgggg
    10501 gcgcaaactg accgcagtta aaccgatcat tattgatgtt aaaaatgcgg gcgcggaatt
    10561 ttacgatcag gaagtcgtgg ttgataaaga tcagctggtt accagccgga caccggacga
    10621 tctgccagcg tttaaccgcg aggcgttacg cctgctcggt gcctgagtcg cgtagccaaa
    10681 tcattttttt accaaagccc agcgtgttgt cggtgaattt gatttcatcg agccggattt
    10741 cccacaccgg ggccgacagc attctggcaa ccggaaagcg acgattgtac gccttgcgcg
    10801 cgaggtcgct ttcctcacct tccagcctgc ggatctcacc tttaaactgc acaccgcgaa
    10861 ttaacgctac cgttttcggc tgaccgttta ccgttccggc aactgccgcc tgcggcccgc
    10921 tcatctgcgc gtggcgcgtt ttttcttccg tcaaaatgta gaaggcgact ttctgcgcat
    10981 caaaaagata aaaggcatta gcgcaccaga gttccccttc ctgctgcaca caccaggtga
    11041 caacatgttg ttttgccagc caacggctga tggcgatgag tgtttccatt gctgttctct
    11101 tttatactgt gggcctgacc ttaacatgcg atgcagacga tgacaccctg gtttctttac
    11161 ttgatccgta ccgccgacaa taagctttat accgggatca ccacggatgt cgaacgccgc
    11221 tatcagcagc accaaagcgg caaaggggcg aaagcactgc gcgggaaagg agaactgacg
    11281 ctggcgtttt ctgctccagt gggcgatcgt tcgctggcgt tacgggcgga atatcgcgtt
    11341 aagcaactga ccaaacggca aaaagagcgt ctggtagcgg aaggcgcagg gtttgcagag
    11401 ctgttaagca gtctgcaaac cccggagatt aaaagcgatt gaaatgctcg tgatactcaa
    11461 ccaggccggt aacgccattc agcgcgtcat ccgctaaacg atgtacctgg aaggcgcttt
    11521 cagtgcccgg ccagcggcaa cgcagatcgt gatgcgccgc cagttcaaag ccgaaacgac
    11581 tgtacagcgc cggatcgccc agcgtcacca ctgcggcata gccgaactca ttaagcgaat
    11641 cgagtccttc atagaccagt tggcgtgcca gcccctgtcc acggtatttt tcatcaaccg
    11701 ccaacggtgc catgccgacc cattgcaggt cttcgccctg cacatcaacc ggactaaatg
    11761 ccacatagcc aatgacctga ccttcgtcat ctgtcgccac cagccccagc gtcagaaagc
    11821 catcttcacg cagatcgtga accagcttcg cttccgcatc actttcgaat gagcgacgca
    11881 gcagggcatc aatacccggc gcatcaatgg gaatttctac tcgaattagc atggttcacc
    11941 taccgatgtc tgtttggttt caggcgcgtt tttcattccc gcctcaacaa aatccgccag
    12001 ttgcagcagc atcatgcgta aggctttcgg catttgctcc agttcaatgg cgtccatcag
    12061 gtttttcaca tacagcccca gctccgtatc gccttcaatc accagccgac gctggaagaa
    12121 gagcgtatcc ggatcttgtt tacgcgccgc aatcatcagc agatcgctgg cgtcggcact
    12181 aaaactcaca tcagcttgcg cgttctggct gaccaccagt ttgccattca ccaccgaggt
    12241 aaaccattgc aggtcaatat cacgcacatg aatacttaac cagcggcctt caagaaactc
    12301 cagctcgcca tcatccagcg cctgacggaa ttgccagctt aagacctgct caagaacctg
    12361 gcgttttagc gcaaatggcg tcagttttac cggtacactc aacagagatg gccccaaatg
    12421 cacaatacgg gaacgcagtt tatccaacac gagttttact ccctgtttca acaatcatcc
    12481 tattttgcca tatcagaaaa ataacatagc ggtataaatc aacaattcca tatgaaattg
    12541 ctgctaccac caatacaact ttaactgcct taaatcaaaa attgtcgcag caaggttaac
    12601 taaaatccca gttcgttaac atttttgcgt tttgatagcg caaccttcag gaaaaattat
    12661 ggagctgctc tgccctgccg gaaatctccc ggcgcttaag gcggccatcg aaaacggcgc
    12721 agatgctgtt tatatcgggc taaaagatga tactaatgcc cgtcacttcg ccggccttaa
    12781 ctttaccgag aaaaaattgc aggaagcggt gagttttgtc catcaacatc gccgcaaact
    12841 tcacatcgcg attaacactt ttgcgcatcc ggacggttac gcccgttggc agcgcgccgt
    12901 ggatatggcg gcgcagctgg gtgccgacgc gctgatcctc gccgacctcg ccatgctgga
    12961 gtacgccgcc gagcgttacc cgcatattga gcgtcatgtg tcagtgcagg cttcggcgac
    13021 caatgaagag gcgattaact tttatcatcg ccattttgac gttgctcgcg tggtgctgcc
    13081 gcgcgtgttg tcgattcatc aggtgaaaca gctggcacgg gttactcctg tcccgctgga
    13141 agtgtttgct ttcggcagcc tgtgcattat gtcggaaggt cgttgctatc tgtcgtcgta
    13201 tctgacgggt gagtcgccca acaccgtggg agcgtgttct ccggcccgtt tcgtgcgctg
    13261 gcaacaaacg ccgcaggggc tggaatcccg cctgaacgaa gtgctgatcg accgttatca
    13321 ggacggcgaa aacgcaggtt atccgacgct gtgtaaaggg cgttatctgg tggacggcga
    13381 gcgctatcac gcgctggaag aaccaaccag tctcaatacc ctggaactgc tgccggagtt
    13441 aatggctgcg aatattgctt cggtgaaaat tgaaggccgc cagcgtagcc cggcgtatgt
    13501 cagccaggtg gcgaaagtct ggcgtcaggc tatcgaccgt tgtaaggccg atccgcaaaa
    13561 ctttgtaccg caaagcgcgt ggatggagac gctcgggtcg atgtccgaag gcacgcaaac
    13621 cactcttggc gcatatcacc gtaaatggca gtgagaaaag caatgaaata ttccttaggg
    13681 ccagtgctgt ggtactggcc aaaagagacg ctggaagaat tttatcagca ggccgccacc
    13741 agcagcgccg acgtgattta tcttggtgaa gcggtatgca gcaagcgtcg ggcaaccaaa
    13801 gttggcgact ggctggagat ggcaaaatcg ctcgccggga gtggtaagca gattgtgctc
    13861 tccacgctgg cgctggtgca ggcatcatct gaactgggcg aactgaaacg ctatgttgag
    13921 aacggtgagt ttctgattga agccagcgat ctcggcgtgg tgaatatgtg cgctgaacgc
    13981 aaactgccgt tcgtcgccgg gcacgcgctg aactgctaca acgcggtgac actgaaaata
    14041 ttgctcaaac agggcatgat gcgctggtgt atgccagtgg agctttcccg cgactggctg
    14101 gtgaatctgc ttaatcagtg cgatgagctg ggcattcgta accagtttga agtggaagtc
    14161 ctgagctacg gtcatctgcc gctggcctac tccgcccgct gctttaccgc gcgttcggaa
    14221 gaccgcccga aagatgagtg tgaaacctgc tgcattaagt atccgaacgg gcgcaacgtg
    14281 ctgtcgcagg aaaaccaaca agtgtttgta ctcaatggca ttcagaccat gagcggctac
    14341 gtttacaacc tcggtaacga gctggcatcc atgcagggcc tggttgatgt ggtccgcctg
    14401 tcaccgcagg gtactgacac tttcgcgatg ctcgacgcct tccgcgctaa tgaaaatggc
    14461 gctgcaccac tgccgttgac ggcgaacagc gactgtaacg gctactggcg gcggctggcc
    14521 gggctggagc tgcaagccta agtaaatatg ctcgtcatac ttcaagttgc atgtgctgca
    14581 tctgcattcg ttcaccccag tcacttactt atgtaagctc ctggggattc actctcttgt
    14641 cgccttcctg caactcgaat tatttagagc acagctcact ttgttaacaa ctttaactac
    14701 tctttaatgc agtattaaag attaatcggt aacaaagtga gctgttatga ctgataaaac
    14761 cattgcgttt tcgctactcg atctggcccc cattcccgaa ggttcttcag cgcgagaagc
    14821 attctcccac tctctcgatc tcgcccgtct ggctgaaaag cgcggctatc atcgctactg
    14881 gctggcggaa caccacaata tgaccggcat tgccagtgct gcaacatcgg tattgattgg
    14941 ctatctggcg gcgaatacca ccacgctgca tctggggtct ggcggcgtga tgttgcctaa
    15001 ccactcaccg ttggtcattg ccgaacagtt cggcacgctc aatacgctct atccggggcg
    15061 aatcgatttg gggctgggtc gtgctccggg tagcgatcag cgaaccatga tggcgctacg
    15121 tcgtcatatg agcggcgata ttgataattt cccccgcgat gtcgcggagc tggtggactg
    15181 gtttgacgcc cgcgatccca atccgaatgt gcgcccggta ccaggctatg gcgaaaaaat
    15241 ccccgtgtgg ttgttaggct ccagccttta cagcgcgcaa ctggcggcgc agcttggtct
    15301 gccgtttgcg tttgcctcac acttcgcgcc ggatatgttg ttccaggcgc tgcatcttta
    15361 tcgcagcaac ttcaaaccgt cggctcgact ggaaaaacca tacgcgatgg tgtgcatcaa
    15421 tattatcgcc gccgatagca accgcgacgc tgagtttctg tttacctcaa tgcagcaagc
    15481 ctttgtgaag ttgcgccgtg gcgaaaccgg gcaactgccg ccaccgattc aaaatatgga
    15541 tcagttctgg tcgccgtccg aacagtatgg tgtgcagcag gcgctgagta tgtcgttggt
    15601 aggtgataaa gcgaaagtgc gtcatggctt gcagtcgatc ctgcgcgaaa ccgacgccga
    15661 tgagattatg gtcaacgggc agattttcga ccaccaggcg cggctgcatt cgtttgagct
    15721 ggcgatggat gttaaggaag agttgttggg atagtgtgtc ttaacgcggg aagccatatc
    15781 cgagctggca acgctgtcct acgtagacct gataagcgaa gcgcatcagg cattgtgtag
    15841 gcagcagaaa tgtcggataa ggcaccgctg attactgata caccggcagt aaattaaagc
    15901 tcgataaaat atggaccagt gcgttgccaa cgccaaacac cagaatcagc gtaatcatcg
    15961 gcttgccgcc ccagacgcgg aatttcgggc tgccaaagcg tttacgcgat gcgcgggcta
    16021 acagcgccgg aacaattgcc gcccagatgg tagctgctaa accagcataa ccaatggcgt
    16081 acaggaatcc gttcgggaac agtagaccac ccacaacagg cggggcaaag gtcagcaatg
    16141 ccgttttcaa gcgccccagg gctgagtcat cgaaaccaaa cagatctgcc agatagtcaa
    16201 acaaacccag cgttacgcca aggaacgaac tcgctaccgc aaagtttgag aacacgacca
    16261 gcagaaggtc caggctacgg ctgttcagca cgccgcttaa tgcctgtacc agcacatcaa
    16321 tattaccgcc cttctctgca ataccgataa actccggacg cgggatgtta cccatcgtcg
    16381 ccagcaacca gatggtatac agcgccagtg ccatcagcgt accgtacacc aggcatttca
    16441 cgatggtttt cggatctttg ccgtaatact tcatcaggct tggcacgtta ccgtgataac
    16501 caaacgacgc caggcagaac ggcaatgtca tcagcagata cggcgcataa gatgcattgc
    16561 tttctgcaac gttgaacaat gtcgcaggct gcacatgccc caacaggctg ccaaaggtga
    16621 ggaagaaggt aatgactttc gcccccagca caatcgccgt catgcggctg acggccttag
    16681 tactcaacca caccacaaac gctaccagca gtgcaaaacc aaaacccgcc gcccgtgccg
    16741 ggacgtttag cgacatctct gcgaaggtgt gatgcagaat cgaaccactg gcagaaatat
    16801 aggcataggt caggatatag agcacaaagg caatggaaat accgttgacc acgttccagc
    16861 ctttgcccag caaatctttg gtgatggtgt caaaactcga accgattcgg taattcaggt
    16921 tagcttccag aatcatcaag ccggaatgca gcatacagaa ccaggtaaag atcagcgccg
    16981 ccattgacca gaaaaaccac gccccggaca tgaccactgg cagagaaaac atccctgcgc
    17041 caataatggt gccgccgata atcaccacgc cgccaagcag cgacggtgac gtttgggtgg
    17101 tggttagtgt tgccatgagg gcttctctcc agtgaaaaat agtgcgactg cgttgttatg
    17161 cattgcactg taccagtaca cgagtacaaa agacagaaaa aaagccccga tggtaaaaat
    17221 cggggctgta tatattattt tacagattgt gttcgctgtt cagcgatgat tacgcatcac
    17281 caccgaaacg acgacgaccg gtagagtcat cacgacgcgg agcgcggcct tcacgacgtt
    17341 cgccgctaaa acgacgacca tcaccacggc caccttcacg gcgttcaccg ctgaagttac
    17401 gaccgccttc acgacgttcg ccaccgaaac cacgaccacc gccacgacgc tcaccgccag
    17461 tatgcggctg tgcatcgccc agtaactgca tgttcatcgg cttgttgaga atgcgagtgc
    17521 gcgtaaagtg ttgcagcact tcacccggca tacctttcgg cagttcgatg gtggagtgag
    17581 aagcaaacag cttgatgtta ccaatgtaac ggctgctgat gtcgccttcg ttagcaatcg
    17641 caccaacgat atgacgaact tcaacaccat catcgcggcc cacttcaatg cggtacagct
    17701 gcatatcgcc aacatcacga cgttcacgac gcggacgatc ttcacggtca ccacgcgggc
    17761 cacggtcgtt acgatcgcgc ggaccacggt catcacggtc acggaattca cgtttcggac
    17821 gcatcggcgc atctggcggt acgatcagag tacgttcacc ctgtgccatt ttcagcagtg
    17881 ccgcagccag agtttcgaga tccagctctt caccttctgc agtcggctga attttgctca
    17941 gcagtgcgcg gtattgatcc agatcgctgc tttccagctg ctgctgtact ttagcggcga
    18001 atttttccag acggcgtttg cccagcagtt ctgcgttcgg cagttctact tccggaatag
    18061 tcagcttcat agtacgttca atgttgcgca gcagacgacg ctcgcggttc tcaacgaaca
    18121 gcagcgcgcg gccagcacga cccgcacgac cggtacgacc gatacggtga acgtaagact
    18181 cagaatccat cgggatatcg tagttaacta ccaggctgat acgctcaacg tccaggccac
    18241 gggctgcaac gtcggtcgca atcaggatgt ccagacgacc atctttcagg cgttccagtg
    18301 tctgttcacg aagcgcctgg ttcatgtcac cgttcagcgc ggcgctgttg tagccgttac
    18361 gctcaagagc ttcagccact tccagagtcg cgtttttggt acgaacgaag ataatcgccg
    18421 catcaaaatc ttccgcttcc aggaaacgta ccagtgcttc gtttttgcgc ataccccaga
    18481 cagtccagta gctctggctg atgtcaggac gggtagtcac gctggactga atgcgcactt
    18541 cctgcggctc tttcataaag cggcgggtaa tgcgacgaat cgcttccggc atggttgcag
    18601 agaacagagc ggtctgatga ccttccggga tctgcgccat aatggtttca acgtcttcga
    18661 tgaagcccat gcgcagcatt tcgtcagctt catccagaac cagaccgctc agtttagaga
    18721 ggtccagagt gccacgtttc aggtggtcca gcagacggcc cggagtaccg acaacgatct
    18781 gcggcccctg acgcagggcg cgtaattgca cgtcataacg ctggccgccg tacagagcaa
    18841 ccacatttac gccgcgcatg tgtttagaga aatccgtcat tgcttcagca acctgtaccg
    18901 ccagttcgcg ggtcggtgcc agcaccagaa tctgtggtgc tttcagctca ggatcaagat
    18961 tctgcaacag aggtaaagag aatgctgcag tttttccgct ccccgtctgg gccataccca
    19021 gaacgtcgcg gccattcagc agatgtggaa tacactctgc ctgaattgga gatggttttt
    19081 cgtaacccag atcgttaagg gcttcaagga taggagcctt caggcccaga tctgcaaaag
    19141 tggtttcgaa ttcagccatg tagtacgtgt gcctcaaaat taatggcggc cagtctacat
    19201 aactcatcat gaaattgatc agcaattttc attgaaaagt gtgaaccggc tcaaagtagg
    19261 tgtattaacg aacaacaacg ccctcacccg ttaaggtgat ggcaatcaaa aaagattacg
    19321 ggctgatgtg tacgtcagct attgctggtc cgattctgcc aggtcatctt ggtcctggcc
    19381 caggagcgat aattccaaca atgcgtatcg gtgctcaaca aagttatgaa cgttgttggc
    19441 aaccgccagt ttgaacagtg ccgtggcgct gtccaaatcc cccagactta ggtagtactt
    19501 acctaaatag aagttggttt cactgagatg ctcagcgagc gaggtgttat ccgttgcgtc
    19561 cgccttgagc ctttccatta acgtttgttc gctaatgttg cccaggtaga actcgacaat
    19621 gttccatccc cactgttcct tatccgattt ttcgaagtgc tgtttcaaca cttctttagc
    19681 ctgcttctca tcgagcttct gctcggcgag ataaagccac agactacgga aaggatcatt
    19741 gggatcgtct tgataaaacg ccagcagatc atcttgcgct aacttgtcac gaccgccgta
    19801 atataatgcg atcccgcgat tcaagtgcgc gtagttgtaa gttggatcaa gctcaagtac
    19861 agaatcaaac gcttcatagg cagcatcaaa attgcctgcc tgcgttaaat atatgcctaa
    19921 gtaattgaat acttcaggca tatccggtcg gattgccagc gcttgcgaaa aatcgttacg
    19981 cgctaatgcc ctcagaccga gactatcata caacactccg cgctcatata aaagctgtgc
    20041 gcgttcgtca tcggttaaag cccgactggc aaggatttgt tccatacgtg ccagaatcac
    20101 ttcctgctgt aaagtcggtt gcaatggtac cgcgaggact tcacttttac gccaggaagt
    20161 attactgcat cctgcaagcg taagtgctgt cgcaacgaaa caccagcgca aaaaaggctt
    20221 catttcccac tcccgaagac cacggttgaa tgaacgtcct gttcccggtt gctaacaagg
    20281 cgtcctgccc ggttaaaagc cccccgccgc agcggagggc aaatggcaac cttactcgcc
    20341 ctgttcagca gccggagctt ccggtgctgc agcaggttga gactgctcag tcgcttcttt
    20401 aatgctcaga cggatacggc cctggcgatc aacttccaga actttcaccg gtacttcctg
    20461 acccatctgc aggtaatcgg tcactttctc aacgcgtttg tcagcgattt gagagatgtg
    20521 gaccagacct tctttaccgc cgccgatggc aacaaatgcg ccaaagtcaa cgatacgggt
    20581 cactttacca gtgtagacgc ggcccacttc gatttctgca gtgatctctt cgatacgacg
    20641 aatagcatgt ttcgctttct cgccgtcggt cgctgcgatc ttcacagtac cgtcatcttc
    20701 gatttcgatg gtagtgccag tttcttcggt cagggcacgg ataacagagc cgcctttacc
    20761 gataacgtct ttgatcttat ccgggttgat cttgatggta tggatacgcg gtgcgaactc
    20821 agagatatcg ccacgcggcg cgttgatcgc ctgttccatt acgcccagga tatgcagacg
    20881 cgcaccttta gcctggttca gcgcaacctg catgatctct ttggtgatac cttcaatttt
    20941 gatatccatc tgcagtgcag agataccgtc gcgggaacct gcaactttga agtccatatc
    21001 gcccaggtga tcttcgtcgc ccaaaatgtc agacagtaca acgtagttgt cgccttcttt
    21061 caccagaccc attgcgatac ccgcaacggc agctttgatt ggcacacctg cgtccatcag
    21121 cgccagagac gcgccgcaca cggaagccat agaagaagaa ccgttggatt cggtgatttc
    21181 agacactaca cgtacggtgt acgggaattt gtccatatcc ggcatgactg ccagcacgcc
    21241 gcgcttcgcc agacgaccgt gaccaatttc acgacgcttc ggagaaccga ccatgccggt
    21301 ttcgcctacg gagtacggag ggaagttgta gtggaacagg aaggtatcgg tacgttcgcc
    21361 catcagttca tcaagaacct gcgcgtcacg agcggtaccc agcgttgcag taaccagtgc
    21421 ctgcgtttca ccgcgggtga acagcgcaga accgtgagta cgcggcagca cgccagtacg
    21481 cacatccaga ccacggatca tatctttttc acgaccgtcg atacgcggtt cgcctgccag
    21541 tacgcggcta cgaacaacgt ttttctcgat agcgtgcaga atttcaccca gttcgttttc
    21601 gtccagggtt tcgtcttcag caagcagcgt cgcgatggtt tcagatttga tgacatcaac
    21661 ctgcgcataa cgctcttgtt tgtcggtgat gcggtaagca tcgctcaggc gagcttcagc
    21721 cagtgcagca acgcgcgcgt tcagcgcttc gtttaccggc tccggctgcc agtcccaacg
    21781 tggtttaccg gcttctttca ccagttcatt gatgttctga ataacaacct gctgttgttc
    21841 atgaccgaac actactgcgc ccagcatctg gtcttcgctc agcagttcag cttcagattc
    21901 aaccatcaga acagcggctt cagtaccggc aacaaccaga tccagtttgc tctctttcag
    21961 ctcgtcctga gtcgggttca gtacgtactg gtcattgatg taacctacgc gagcagcacc
    22021 aatcggacca ttgaacggaa taccagacag agacagtgct gcggaagcac caatcatcgc
    22081 gacgatatcc gggttaactt gcgggttaac agaaaccacg gtggcgataa cctgaacttc
    22141 gttgacgaag ccttccggga acagcgggcg aatcgggcgg tcaatcagac gcgcgatcag
    22201 ggtttcgcct tcgcttgggc ggccttcacg acggaagaag ctacccggga tacgaccagc
    22261 agcgtaggta cgctcctgat agttaacggt cagtgggaag aagtcctgac ctggtttggc
    22321 ttttttctgg ccaacaacgg taacaaatac cgcggtgtca tccatgctaa ccataacagc
    22381 ggcagtagcc tgacgagcca tcatgccggt ttccagagtc acggtgtgtt ggccgtactg
    22441 gaatttacga acgatcggat taagcaatgt aatatccttt ctctttctta gacagtacct
    22501 tacggcactg gtgttaatac ccgatcttct gcgcatcctc gcgactaatg acaaccctaa
    22561 cccagctcta tgtgggtaaa gcttctcatt agccgcgcga acctctgcaa cggaagatca
    22621 ttcatagcaa caatacatta gtttccagtg aattgctgcc gtcagcttga aaaaaggggc
    22681 cactcaggcc cccttttctg aaactcgcaa gaattagcga cgcagaccca ggcgctcgat
    22741 gagctgggtg taacgtgcta cgtctttacg tttcaggtag tcgagcagtt tacgacgctg
    22801 agaaaccatg cgcagcagac cacgacggct gtggtgatct tttttgtgct ctgcaaagtg
    22861 gccctgcagg tggttgatct gtgcagtcag cagtgctacc tgaacttcgg tagaaccggt
    22921 gtcgtttgcg tcacgaccaa actcagaaac gattttagct gttgcttcag tacttagaga
    22981 cattttaaaa ctccaaagta tatagaatga aaggacgccg atctctaatt cagcgatccc
    23041 agtgtacgtt acgcaaagtg ttaaacaatt tacgcgacgt taagcggcag tattctactc
    23101 gtagcgacct gttatcgcaa gacggttaac attacgccgg gtattcaacc accaggcgac
    23161 gaggcgcaac gcggccttca tcgtcaattt cgcccatacc gataaatttg ccgttctcac
    23221 cttctgtaac gcgaaccagt ccttccagcg gcgcaccgga tgtacgaact gggttaccat
    23281 ttttgaagta aacagaagac gttaacggaa gattcaccac cgggtagtcc gaagctggac
    23341 tgtccattgg catcagtaat ggatcaagta actccgcagc cggaatatcc tgctgttcgg
    23401 cttgttcgac cagttcacgc agatgttcca gggtcaccat ccgttcaacc ggatatttac
    23461 ttaccgccag acgacgcagg taaataacat gcgcgccaca accgagtttt tcacccaggt
    23521 catcaatgat ggtgcggata taagtgcctt ttgagcagtg aatttccagc tccagctcat
    23581 tgccttcgtg gcgaataaac agcaattcat aaacggtaat cgggcgcgct tcacgcggta
    23641 cttcaatacc ctgacgcgca tattcgtaca gttttttacc ctgatatttc agtgctgaat
    23701 acatcgaagg gatctgttcg atatcgccac ggaaagtatc cagcgccgcc gccagttgct
    23761 ctgcgctaaa ggttaccgga cgttcttcaa cgatttgtcc gtcggcatca gaagtatcgg
    23821 tacgctgtcc aagacgcgca atgacccgat agcgtttgtc ggagtccagc agatactggg
    23881 aaaacttcgt cgcttccccg aggcaaattg gcaacatgcc agttgccagc gggtccagcg
    23941 caccggtatg cccggcacgg ttggcgttat aaatccgttt cactttttgc agcgcatcat
    24001 tgctggacat accctgcggt ttatccagca acaaaacgcc gttaatgtcg cgaccgcgac
    24061 gacgaggacg actcattagt cctccttgct gtcgtccggg ttaacacgac gttcttcgtc
    24121 atgtttgacc acgctggtca ccaggtttga catgcgcatc ccttcaacca gagagttgtc
    24181 gtagaagaag gtcagttccg gcacgatacg caggcgcatc gctttcccca gcaggctgcg
    24241 gatgaaacca gaagcttctt gcaacgcttt gatgcccgct ttaaccgcgt cttcatcttt
    24301 gtcgttgagg aacgtcacat atactttggc atacgccagg tcgcgagaca tttcgacacc
    24361 ggaaacggtg gtcatcatgc ccaggcgagg atctttaatt tcacgctgca ggatgagagc
    24421 gatctctttt tgcatttcct gcgctacgcg ctgcgggcga ccaaattctt tcgccataat
    24481 aaattctcct gacaaaaaag gggctgttag ccccttttta aaattaattt caggtggaag
    24541 ggctgttcac gttggcctga taagacgcgc cagcgtcgca tcaggcaatc catgcaggat
    24601 gtggcgtaaa cgccttatcc cgcatggaac cctaaaaacc ttaagcgatg gtacgttgga
    24661 tttcgatgat ttcgaatact tcgatcacat cgccagtgcg gacgtcgttg tagttcttaa
    24721 cgccgatacc acattccata ccgttacgga cttcgttaac gtcatctttg aagcggcgca
    24781 gggactccag ctcgccttcg tagataacca cgttgtcacg cagaacgcgg atcgggttgt
    24841 gacgtttaac cacaccttcg gtaaccatac agcctgcgat ggcaccaaat ttcggtgatt
    24901 tgaacacgtc acgaacttcc gccagaccga taatctgctg tttcagttcc ggagacagca
    24961 taccgctcat cgccgctttc acttcgtcaa tcaggttata gatgacggag tagtaacgca
    25021 gatccaggct ttccgcttca atcactttac gtgcagaggc atcagcacgt acgttgaagc
    25081 caaccaggat ggcgttggac gccgcagcca gggtggcgtc ggtttcggtg ataccaccta
    25141 cgccagaacc gatgatcttc actttaactt cgtcagtaga cagtttcagc aaggagtcgg
    25201 agatcgcttc cacagaaccc tgtacgtctg ccttcaggac gatgttcact tcgtgaactt
    25261 cgccttcggt catgttggcg aacatgttct cgagtttaga tttctgctga cgcgccagtt
    25321 taacttcgcg gaatttaccc tgacgataca gtgcaacttc acgcgctttc ttctcgtcac
    25381 gtacaacggt aacttcatca cccgcagccg gtacgccgga caggccgagg atttccaccg
    25441 gaatggacgg acccgcttcc agcacttcct gacccagttc gttacgcatc gcacgaacac
    25501 gaccgtattc gaagccacac agaacgatat cgcccttgtg cagagtacct tcacgtacca
    25561 gaacggtagc aaccggacca cgacctttat cgaggaagga ttcgataacc gcaccgctcg
    25621 ccataccttt acgtaccgct ttcagctcca gaacttccgc ctgcagcagg atagcgtcca
    25681 gcagttcatc gataccagta cccgctttcg cagacacatg tacgaactgg ctttcaccgc
    25741 cccactcttc cggcaggatg ccgtactggg agagttcgtt cttaacgcga tccggatcgg
    25801 cttctggttt atcgatcttg ttcactgcaa cgaccaccgg aacctgcgcc gctttcgcgt
    25861 gctggattgc ttcgatcgtc tgcggcatta cgccgtcgtc ggcagcaaca accaggacta
    25921 cgatgtccgt tgcctgcgca ccacgagcac gcattgaagt aaacgcggcg tgccctgggg
    25981 tgtccaggaa ggtgatcatg ccgttttcag tttcaacgtg gtatgcacca atgtgctggg
    26041 taatgccgcc cgcttcgcca gaggccactt tcgttgaacg aatgtagtcc agcagagagg
    26101 tcttaccgtg gtcaacgtga cccatgatgg tcacaaccgg cgcgcgcggt tcagccgcag
    26161 cacccgtgtc acggtcgctc attaccgcct cttccagctc gttttcacga cgcaggataa
    26221 ctttatggcc catctcttca gcaaccagct gtgcggtttc ctgatcgata acctggttga
    26281 tggttgccat tgcgcccagt ttcatcatcg ctttgatgac ctgagagcct ttaaccgcca
    26341 tcttgttcgc cagttcgcca acggtgatag tttcgccgat cacaacgtca cggttaacgg
    26401 cctgagcagg cttctggaag ccttgctgca gggaagaacc tttacgtttt ccgcctttac
    26461 cgccacgtac tgctgcgcgt gcttcttcac gatcagcttt tgattcagcg tgtttgttac
    26521 ctttcttcgg acgcgctgct ttcgcgttac gaccacggcc acggccgcct tcgacttcac
    26581 gatcgctttc gtcttcggcc tggcgagcat gttgagaagt agtgacgtga taatcgctgg
    26641 aatcttcagt cggttccgcg ttatcagtcc atttgttttc ttccgccata cgacgtgctt
    26701 cttcagcaac gcgacgtgct tcttcttcga gtttacgacg cgcttcttct tcagctttac
    26761 gcttgagctc tgcagcttcc tgctcacggc gggctttttc agcctgggcg tttttagtca
    26821 tatcgtcttg ttgattgctc actttgtctt tttccgcagc ttcacgtttc gcttgttcag
    26881 cagcttcacg cttagcttgt tctgcggcct cacgttcagc tttttgttgc gcctcgcgtt
    26941 tagccgattc ttctgcctca cgacgggctt gctcttccgc ttcacgctgc gcttgctctt
    27001 ccgctgcaag gcgttcagcc tcttgcggat cgcgtttcac aaaggtgcgt ttcttgcgga
    27061 cttcgatttg taccgatttg ctttttccac cggtaccagg aatgttaagg gtgctgcgtg
    27121 ttttacgctg cagcgtcaat ttgtccgggc ctgaattttt ctgattcagg tggtcaatca
    27181 aagtctgttt ctcttgtgca gacacagagt cgtcagcaga cttccggata cctgcatcag
    27241 caaattgctg taccaggcgt tccacggagg tctgtcgctc tgcggccagc gttttaatcg
    27301 ttacatctgt catgctgttc cttcctgcta cagtttatta cgcttcgtca ccgaaccagc
    27361 aaatattacg ggcagccata atcagtgctc cggctttttc gtcggtcaac ccttcgatat
    27421 cagccagatc atcaatgccc tgttcggcga gatcttccag cgtacaaacg ccacgggcgg
    27481 ccagtttgaa tgccaaatca cgatctaccc cttcaaggtt cagcagatcg tcagccggtt
    27541 tgttatcacc gaggctttct tcctgggcct gtgcaatggt ggccagtgca tttttagcac
    27601 gctcgcgcag tgcttcaacg gtcggctcat caaggccttc gatttccaac agctctttca
    27661 tcggcacata ggccagttct tccagcgtcg agaagccttc ttctaccaga acagtcgcga
    27721 agtcttcgtc gatgtcgaga tatttggtga aggtgtcgat cgctgcgtgc gcttccgcct
    27781 gatgcttagc ctgcaggtcg tcaacggtca tcacgttgag ttcccaaccg ctcagctgcg
    27841 aagccagacg cacgttctga ccgttacggc caatcgcctg cgccaggtta ccggcttcaa
    27901 cggcgatatc catggtgtgt ttatcttcat ccaccacgat agaagcaacg tctgccggtg
    27961 ccattgcgtt aatcacaaac tgcgccgggt tatcatccca caggacgata tcgatacgct
    28021 cgccgcccag ttcagtagaa accgcctgaa cacgcgcgcc acgcatacct acacaggcac
    28081 ctaccggatc gatacgttta tcgttggttt tcaccgcgat tttcgcacga gaacccggat
    28141 cgcgagccgc tgctttaatt tcaatcactt cttcgccgat ttctggcact tcaatacgga
    28201 acagttcgat cagcatttcc ggcttggaac gagtgacgaa cagttgcgcg ccacgcgctt
    28261 ccgggcgaac ggaatagagc acgccacgaa cgcggtcgcc agggcggaag ttttcacgcg
    28321 gcagcatatc ttcgcgcagg atcacggctt cagcattgtt gcccagatcc agagagatgt
    28381 tgtcgcggtt tacttttttc accacgccgg tgatgatttc accttcgtgt tcacggaact
    28441 gatcaaccac catcgcacgt tcggcttcac gcactttctg cacgataacc tgttttgccg
    28501 tctgggtagt gatacggtca aaggtaacag actcaatctg atcttcaacg taatcgccca
    28561 ggttcaggct ttcatcttca taacgtgcgg cttcaagggt gatttccttg gtcggctggg
    28621 tgacttcatc aacaactaac cagcgacgga aggtgtcaaa atcaccgctt ttgcgatcga
    28681 tctgtacgcg gacgtcgatc tcttgttcat attttttctt tgttgctgtc gccagcgcgc
    28741 tttccaatgc ttcgaaaatc ttctcgcgag gtagcgcctt ttcattggat acggcttcaa
    28801 ctacagccaa aatttctttg ttcatcgcgg gcttttcacc tcatccagac tattaaaagt
    28861 ggggaaccag gttcgccttc tggatattac tcagcgcgaa cacttcatct ttaccttcga
    28921 cggtaactgt gatcatttca ccgtctaccg ctttgataac gccctgccat ttacgacggt
    28981 tttgtaccgc catacggaga accagagtca cctcttctcc gacaaaacgg gcgtagtgtt
    29041 cagccgtgaa cagtgggcga tcgagacccg gtgaggagac ttccaggtta taagcaacgg
    29101 tgatgggatc ttcaacatcc agcacagcac ttacctggtg gctcacatca gcacaatcat
    29161 caacattgat gccatcttca ctatcaatat agatgcgcag tgtggatgtg cgaccgcgaa
    29221 taaattcgat gccaaccagt tcaaaaccca gggcctcaac tggcgcagta atcatctctg
    29281 ttaatttttg ctctaatgtg gacaagccca cccccaagac ataaaaaaag ggcctaaagc
    29341 ccagttattc tgtagtcaga taacaaaaaa ccccgataaa tcggggcatt atataactga
    29401 accctataac cgcaactgcg gtctggagca ctttccagaa ggattttttc aaatcccact
    29461 acgaaggccg aagtcttcac agtatatttg aaaaaggact ctaagggaaa gtggttgcgg
    29521 gggccggatt tgaaccgacg accttcgggt tatgagcccg acgagctacc aggctgctcc
    29581 accccgcgcc tgaaacgtgg caaattctac tcgttttggg taaaaaatgc aaatactgct
    29641 gggatttggt gtaccgagac gggacgtaaa atctgcaggc attatagtga tccatgccac
    29701 attttgtcaa cgtttattgc taatcacgtg aatgaatatc cagttcactt tcatttgttg
    29761 aatacttttg ccttcccctg ctctccctta agcgcattat tttacaaaaa acacactaaa
    29821 ctcttcctgt ctccgataaa agatgattaa atgaaaactc atttattttg cataaaaatt
    29881 cagtgagagc ggaaatccag gctcatcatc agttaattaa gcagggtgtt attttatgac
    29941 gacgattctc aagcatctcc cggtaggtca acgtattggt atcgcttttt ccggcggtct
    30001 ggacaccagt gccgcactgc tgtggatgcg acaaaaggga gcggttcctt atgcatatac
    30061 tgcaaacctg ggccagccag acgaagagga ttatgatgcg atccctcgtc gtgccatgga
    30121 atacggcgcg gagaacgcac gtctgatcga ctgccgcaaa caactggtgg ccgaaggtat
    30181 tgccgctatt cagtgtggcg catttcataa caccaccggc ggcctgacct atttcaacac
    30241 gacgccgctg ggccgcgccg tgactggtac catgctggtt gctgcgatga aagaagatgg
    30301 cgtgaatatc tggggtgacg gtagcaccta taaaggaaac gatatcgaac gtttctatcg
    30361 ttatggtctg ctgaccaatg ctgaactgca gatttacaaa ccgtggcttg atactgactt
    30421 tattgatgaa ctgggcggcc gtcatgagat gtctgaattt atgattgcct gcggtttcga
    30481 ctacaaaatg tctgtcgaaa aagcctactc cacagactcc aacatgcttg gtgcaacgca
    30541 tgaagcgaag gatctggaat acctcaactc cagcgtcaaa atcgtcaacc caattatggg
    30601 cgtgaaattc tgggatgaga gcgtgaagat cccggcagaa gaagtcacag tacgctttga
    30661 gcaaggtcat ccggtagcgc tgaacggtaa aacctttagc gatgacgtag aaatgatgct
    30721 ggaagctaac cgcatcggcg gtcgtcacgg cctgggcatg agcgaccaga ttgaaaaccg
    30781 tatcatcgaa gcgaaaagcc gtggtattta cgaagctccg gggatggcgc tgctgcacat
    30841 tgcgtatgaa cgtctgttga ccggtattca caatgaagac accattgagc agtatcacgc
    30901 acatggtcgt cagttgggcc gtctgctgta ccaggggcgt tggtttgact cccaggcgct
    30961 gatgctgcgt gactctctgc aacgctgggt tgccagccag atcactggtg aagttaccct
    31021 ggagctgcgc cgcgggaacg attattcaat cctgaatacc gtctcagaga acctgaccta
    31081 caagccagaa cgtctgacga tggaaaaagg cgactcggtg ttctcgccag atgatcgtat
    31141 tggtcaattg accatgcgca acctggatat caccgatacc cgcgagaaac ttttcggtta
    31201 tgccaaaact ggcctgctct cctcctctgc cacttcaggc gtgccgcagg tggagaatct
    31261 ggaaaacaaa ggccagtaat tcgacatcaa ccctgccctt caggggtggg gttaccttgc
    31321 ttatcctctg taaactcctt tctgagcatc acataatcga aaacatcatg gtttgcatcc
    31381 acaacccgtg agtctccttt atagtgtgca atcacttcct caagcgtttg cggctgttcc
    31441 ggctttgtta cccccatcca cgcattaata aggtaattat tgtaagctgt cgaaaagatg
    31501 ttgttttccg ttgtgcgatc cacgccatcc ccaagaacgg ggctatacca gataaacatc
    31561 gggacatgat atgcctgctg gctagcctcc ctgcctccat gaaaatagac gttcttcttc
    31621 gtagggtcac gttccaggcc gtggtcggca aaatacatga ccgaggcgcg gcgatctttt
    31681 aataattcaa aaacctgacc tagcaaacta tctgtgtaat gaatggagtt gtcatagcag
    31741 gcatcctgat cgtcctgcgg ttgaaacacg gcgctggatt gcggataggc gctacaagcc
    31801 ggttcatggc ttccatttaa atgaagaaca atcagtttct tttgctgcgt attttgctgt
    31861 aatgcttgcg ataaatgcgg caacaacaat tcatcaaatc ctctgacata aactgtttcc
    31921 atggcgcgca tggcgatact ggtaactgct gtaccattct gccgaaaagc ggattgcgag
    31981 cttagccaga aagtctgaaa tcctgcttga ttagccatat taataatgtt gtcggggtaa
    32041 ttatgaatgt catgactcaa aacagaatca gcagttaaag aaaggggaac cgacagcgca
    32101 gtgtaaggtg cgccgcttat tgcttgatta aacagtttga tctgttttct ttgtgcttca
    32161 acttgcggtg tcgtagagcg tgtatatcca tacaaagaca tattgtcgac acgtacagac
    32221 tcccccacaa tcaacacgta ggtatcaata cctgtatccc tgactgataa ttgaaaatac
    32281 ggcaccgtgt ttgcaattga gagtaatctt tgatgctctt tcgctgctaa agcaaaatag
    32341 ttgagattga aaaatggcgt ataggtagca aatcgcgacg ctagtattta tggactgaac
    32401 gcttttttat tttttgcatc tttataagca aattgacaag cggaaaataa actgcccgaa
    32461 atgacaatca gcaataatat tcctgtcact ttttttgtcg gcaaggaaac atcatatttt
    32521 attattactg ccaaaaaaag aagggataaa aaagcaaagg cacatagata agggatatac
    32581 atccccagca ttttgacaac ttcatccgga tcactctgga gcacactaat cgcaaatcca
    32641 tcattgaaag ttgtaccaaa ggtacaccat gaatagaggc taatactcat gtctgccgca
    32701 gtcagtaaca caaatggtgc ggcaacaagc aggcgaagcg gaagggatga tatacgtttt
    32761 acaaccagga tggtcaggac gaagaacagc aatcgctgaa ccatatgcgc gccggaagag
    32821 gcgactaagt tcgtaatacc aaaaagaata acacaaagat acaacaacca atgagattta
    32881 aaacttctag caaatttgtt gaatactgtc atccagaaat ccaagttcac aaccaatata
    32941 aacaattatc aacaatgttc aattttatat cagacatgtt tttcaactct gatccatatc
    33001 agaccgaaac actgttagtt ttttcttttt aaatgatgcg gccattgcgt tctcagtaat
    33061 taaataacat tatcaatgcg tctctaaaca ttacgcagaa cgtagccatc agtagttata
    33121 ctgacaggag caggatggtt tatcgagaag taaacatatc cgtattcgta ccggaactgg
    33181 agttgacaca ataaagtgcc aattatgtca gcagaaggga aaagtttata actaaggcgt
    33241 accggcacca tcgtttcaag gtaccagcta cgagtaaagc aactggacga gatacagata
    33301 cctgacaacc attcctcaga ccaggaccaa aacgaaaaaa gacgcttttc agcgtctctt
    33361 ttctggaatt tggtaccgag gacgggactt gaacccgtaa gccctattgg gcactaccac
    33421 ctcaaggtag cgtgtctacc aattccacca cctcggcacg gatactactt tttagttcgg
    33481 gatatcgctg gtcggcttag ccggagcagc tggttgagtt tgttcggttt tcaccggtgc
    33541 actcagattt tcccattcgc tacctttatt ggttttgttg ctattgatgt tacccagcac
    33601 cagactgatg atgaagaata acgttgccag cagcgccgtc atgcgggtca tgaagttacc
    33661 agaaccactt gaaccaaaca gcgtagcgga agcgcctgct ccgaaggagg ctcccatatc
    33721 agcgccttta ccttgctgca gcatgatcag accaacaagg ccaattgcca caataaggaa
    33781 aactactaaa agagcttcgt acataatcaa cctgttcctt gcggagttgc cgcgtaccaa
    33841 tgcttcaacc aataaagcgg gagtttttaa tctttcccac tgaagcgggt gtgaatacta
    33901 accaaagcga atgaccttcg caagggcaat tttatcgcat tgtatcaact gcggaaaaaa
    33961 acagcaaaac cgatgtgttc gctgagaaaa aaggcgacaa tcgccgcctt tttagccagt
    34021 tatctaacgc tttaaacggc ttttactgca tcggcgatgc ggtgtgcaaa ttcagtcacc
    34081 tgcgcttcgt cttcgccttc caccatcacg cgaattaacg gttcggtgcc ggatttacgc
    34141 agcaacacgc gtccacggtt gcccagcgca gcttcaacct ctgcggtcac ggctttaact
    34201 gactcatgct caagtggatc gccgctacct gcggtgtaac gtacgttaac cagaatctgc
    34261 gggaacattt tcatgccgct gcaaaggtcg tgcaggctca tatggttacg tgccatcgcc
    34321 gccagcacct gcaagccagc aacgatgccg tcaccggtag tagttttatc cagcaggatc
    34381 acatgaccgg aattctctgc accgatacgc cagcctttct cctgcatttt ttccagtacg
    34441 tagcggtcac ccactttcgc gcgcgcaaat ggaattccta actgtttcag cgccagttca
    34501 agccccatgt tgctcatcaa tgtacccaca gcgccaccac gcagctggcc ctgacgaaga
    34561 ccttcacgcg cgatgatata catgatctga tcgccatcga ctttattgcc ttcatggtca
    34621 accataatca cgcgatcgcc atcgccgtcg aaggcaatac cgagatccgc tttttcagcc
    34681 agcacacgag cctggagcgc gcgaacgtcg gtagccccca cttcggcatt gatgtttaca
    34741 ccgtttggct cacaaccgat agcgataacg ttcgccccca gttcgcgcag cacgttcggc
    34801 gcgatgtgat aagtcgcacc gtttgcacaa tccaccacaa tcttcagttc actgaggcta
    34861 agttcgttcg ggaacgtggc tttgcaaaac tcgatatagc gacccgcggc atcaacgata
    34921 cggctggctt tacccagttc tgccgaatca acgcagctga tctccttttc catttccgct
    34981 tcgatggcct cttctaccgc atccggcagt ttggtgccgt cgatagagaa gaatttaatg
    35041 ccattatcgt agaacgggtt atgcgatgca gatatcacaa ttccggcctc tgcgcggaag
    35101 gtacgcgtca gataagccac ggccggtgtt ggcatcgggc cagtgaagag tgcggaaagg
    35161 cccgctgccg ccagacccgc ttccagtgct gactccagca tatagccaga aatacgcgtg
    35221 tctttaccaa taataatctt acgggagccg tggcgcgcca gcactttacc cgcggcccaa
    35281 cccagcttaa gcacaaaatc aggtgtgatc ggcgcatccc ctacacgacc acgaatccca
    35341 tcggtaccga aatatttacg attactcata gcgtttgttt tcctttgcag acagagtggc
    35401 ttccaccacc cgcatcgctt ctacggtttc tttgacgtca tgaacacgaa tgatgtgcgc
    35461 gccttgcatt gcggcaatga ccgcacaggc cagactaccg ctcaggcgct cggacggccc
    35521 cacgttcagc agctgcccaa tcatcgattt tcgtgacata cccaccaaca gcggcaggtt
    35581 gaaatggtga aattcagcca ggcgcgccag taatgaatag ttatgggaga gatttttacc
    35641 gaaaccgaat ccggggtcga gcaacaattt ctcttttgcg atacccgcct gctcgcaacg
    35701 tgctatttgc tcaataaagt agcgattcac ttctgcaaag acatcgtcat acttcggagc
    35761 ttcctgcatg gtttttggat ttccctgcat atgcatcaga caaaccggta aaccggtttc
    35821 tgcagccgcc tccagagcgc caggttcgga aagggagcgg atatcattaa taatgtgagc
    35881 gccaactttc gctgactcac ggatgacttc tggtttggat gtatcgaccg agatccagac
    35941 ttcgaagcgt tgagcaattg cctcaaccac aggaataaca cgttgcaact cttcttcaac
    36001 gctaacttcc gccgcccctg ggcgcgtgga ctcgccacca acgtcaatga tcgtcgcgcc
    36061 agcattgatc atcagattcg catgtttcac cgcatctatc agcgagttat gcgtgccacc
    36121 atccgaaaag gaatcaggcg tgacgttgag gatccccatt acgtgaggat ggctaaggtc
    36181 cagtgaagta ccctgggcaa agagtttcat gatgttatcc ctggtatgaa ttgataagaa
    36241 aaaaccccgg agcacgcccc ggggttttcg gtacaaatac agtcatctga tgcgggaact
    36301 tacttgtcgc ctaactgctc tgacatggtg ttacccgggt tcggcgtacg cggttcatca
    36361 accggacgag gagcctttgg actaccattg tcgccagaat tgttagaagc gcctggttct
    36421 tcccagcccg ctggcggacg tacatcgcga cgtgccatca ggtcatcaat ctgcggtgcg
    36481 tcgatagtct catatttcat gagagcatct ttcatcgcat gcagaatatc catattgtcg
    36541 gtcagaagct gacgcgcacg attatagtta cgctcaatca gtgctttcac ttcctggtcg
    36601 atgatacgtg cagtttcatc ggacatatgt ttcgctttcg ctacgctacg gccgaggaat
    36661 acttcacctt cttcttccgc gtacagcagt ggacccaatt tctcagagaa gccccactgg
    36721 gtcaccatgt tacgtgccag gttggtcgca actttaatat cgttggacgc accggtagaa
    36781 acatgttccg gcccgtagat gatctcttct gccagacgac caccgtacag cgtagaaatc
    36841 tggctttcca gtttctgacg gctggcgctg attgcgtcgc cctcaggcaa gaagaaagtc
    36901 acacccagcg cacgaccgcg tgggataatc gtcactttgt gcaccggatc gtgttccggc
    36961 accaggcgac cgataatcgc atgacccgct tcgtggtaag ccgttgattc tttctgcgct
    37021 tccgtcatca ccatggagcg acgttccgca cccatcatga ttttgtcttt cgctttctcg
    37081 aactcaacca tcgacacaac gcgtttgttg ccacgagcag cgaacagtgc cgcttcgttc
    37141 accaggttcg ccaggtcagc accggagaaa ccaggagtac cacgggcaat gattgccgcg
    37201 tcgatatcgg gggccaatgg tacgcgacgc atgtgaactt tcaggatctg ctcacgaccg
    37261 cgaacatctg gcaagccgac cacaacctga cggtcgaaac ggccaggacg cagcagggcc
    37321 gggtcgagaa cgtccggacg gttagtcgcg gcgataacga tgataccttc gttaccttcg
    37381 aagccatcca tctcaaccag catctggttc agagtctgtt cacgttcatc gtgaccaccg
    37441 cccagaccag cgccacgctg gcggcctacg gcgtcgattt catcgataaa gatgatgcac
    37501 ggtgccgctt tcttcgcctg ttcgaacatg tcacgaacac gggatgcacc cacaccgacg
    37561 aacatttcta cgaagtcaga accggagata gtaaagaacg gaactttcgc ttcgcctgca
    37621 atcgctttcg ccagcaacgt tttaccggta cccggaggac cgaccatcag gacgcctttc
    37681 gggatcttac cgccgagttt ctggaagcgg ctcggctcgc gcagatactc taccagttca
    37741 gcaacttctt cttttgcttc gtcgcagccc gcaacgtcag caaaggtcgt tttgatctga
    37801 tcttccgtca gcatgcgcgc tttgctctta ccaaacgaca tggcaccttt gccaccgccg
    37861 ccctgcattt gacgcatgaa gaagatccag acaccaatca gcaacagcat cgggaaccag
    37921 gagatgaaga tagaagccag caggcttggt tcttcaggcg gttcaccgac aaccttgacg
    37981 ttcttggtca acaggttatc cagtaatttc ggatcctgaa ccggaatgta agtggtataa
    38041 cggttactat ctttcttggt aacgttgatt tcacgtccgt tgatacgcgc ttcacgaacc
    38101 tggtcgttat tgacctcttg taggaaggta gagtaatcca ccttacggcc attagactcg
    38161 ctgggcccaa agctctggaa tactgacatc agcacaacgg caatgaccag ccagagtatt
    38221 aggtttttcg ccatgtcact caagggatta acctcttatt acaactgtgt taaaaacagc
    38281 gtcaggatac tctatatcca gcatctttca aactttcgtc tgaaatctcc cggttagggt
    38341 ttacgcccgg tcgctacaat atacacttcc cgcgaacgtg cacgagaaga gtccggctta
    38401 cgaactttga ccttcgtaaa cagggagcga atttccctta gatactcatc gaaaccttcg
    38461 ccctggaaca ccttcactac aaaactgcca cctggcgcta atacatcacg acacatttct
    38521 agcgccagtt ccgccagata catggcacgg gggatatcca ccgccggtgt tccgctcatg
    38581 tttggtgcca tatcggacat gacaacctgg actttgctgt cgccaacgcg ctccagcagt
    38641 gctttcatca ccagttcatc acgaaaatcg ccctgaagaa agtccacacc aacgatagga
    38701 tccataggta aaagatcgca agcgatgatg cggcctttgc cgccaatttg ggtgaccaca
    38761 tattgtgacc aaccacccgg agcagcacca aggtcgacaa ccgtcattcc cggtttaaag
    38821 agtttgtcac tttgctgtat ttcatcaagt ttaaaccagg cacgggaacg taaccccttt
    38881 ttctgtgcct gttgaacata tttatcgcta aagtgttcct gaagccagcg gctggagctg
    38941 gcagaacgct tcttacctgt catttaactt tcccatgggg ataactcatc gtaaccaatt
    39001 gcgtaaattt ttacgcgcct atttggtgat atatgggaga tggcggtaga atgacccgtt
    39061 ttcaatccca acgtaagcaa aaaatacgat gaatctgagt actaaacaaa aacagcacct
    39121 gaaaggtctg gcacatccgc tcaagccagt tgttctgctt ggcagtaatg gtttgaccga
    39181 aggggtgctg gccgagattg aacaagcgtt agagcaccat gaactcatca aggtgaaaat
    39241 cgccaccgaa gatcgcgaaa ctaaaacctt gatcgtggaa gctatcgtgc gcgaaaccgg
    39301 cgcctgtaat gtacaggtca tcggtaaaac gctggtgctt tatcgcccaa ctaaagaacg
    39361 taaaatctcg ctgccacgct aagattatcc taaagttaca cacatttgct gtgtaaaacg
    39421 aggggttttc cgcaggcagg agagcaaaat gccacgctct gttcgttgat aaaaggccgc
    39481 atagcggcct ttttcctttc tttacaatac atcaacatct tgagtattgg gtaattctta
    39541 caggtattcc accttaatta cttcaaattc tacttcgccg cccggcgttt tgatgaccac
    39601 aacatcatct tcttctttgc cgatcaggcc acgagcaata ggtgagttta cagaaatcag
    39661 gttttgttta aagtcagctt cgtcatcgcc aacgatgcga taagtctgtt cttcgtcaga
    39721 atccagattc agcaccgtta cggtagcacc aaaaataacg cgcccattgt tgggcatttt
    39781 ggtgacatca atcacctgcg cgttcgacag cttggcttcg atgtctttga tacggccttc
    39841 gcagaagccc tgctgttcac gagctgcgtg gtattcggcg ttttctttca ggtcgccatg
    39901 ctcacgcgct tccgcgatag cagcaatgat ttcaggacgg cgcacagatt tcagaaaatc
    39961 cagctcttcg cgtaattttt cagcgccgcg taaggtcatc ggaatagctt gcatttgtta
    40021 tacctcttga atattcctga tggggcaagt cttcacccac cccggctgtt cggcctgccc
    40081 ggcataactc ctcaccggga ccagaagcaa aaaaataccg acccgggtac aagtcccagg
    40141 tcagctacaa ttcacatttt gatagtcatt ttaccctgaa gttcccgaag ggtcatcgtt
    40201 tactttatag ggcgttgcgc cgtagtatga cggctcgatt ccaggttgtt agcgcgagat
    40261 tatgcgattt tccagattta tcatcggatt gaccagctgt atagcgttca gtattcaggc
    40321 cgcgaatgtt gatgagtacg ttactcaact ccccgctggc gtcaaccaaa tcgatggcaa
    40381 tgtgttgata gatacctcca ttttcgccag ccacgataaa gcccccggct ggccatggaa
    40441 tgacatgaca caatgcttta gcgctccgcc tgccgccgcc atagttgacc gcaactgttt
    40501 ctccgtctcg ctctacagtg cccaaaaacc cggtgatatg gcctttatac gcgtggcatc
    40561 ttattacccc gttacgatgt tcagccaggt acgcaccctc ccccgtggtt ctgccgaagc
    40621 gcaatactgc gaactggatg tggtgccagg cgacctgaac cgctttacgc tgacgggatg
    40681 cctgccacaa cgttctgagc cgctcccgtt ggcttttgcc gtacaggatg gagccagcta
    40741 tgccggggca attctgaaag atgagttaaa acaggcgggt atcacctgga gtggaacact
    40801 gctgcgccag actcaggtta acgaacctgg aacggtagtt gccagtaaac agtcggcccc
    40861 gctgcacgat ctgcttaaga ttatgctgaa aaagtcagac aacatgatcg ccgatacggt
    40921 tttccgcatg ataggccatg cgcgcttcaa tgtgcctgga acatggcggg ccgggtcgga
    40981 cgccgtgcgt cagatcctgc gccagcaagc cggtgtcgat attggaaaca ccattattgc
    41041 cgatggttca gggctttcgc ggcataacct gattgccccc gccaccatga tgcaggtgct
    41101 gcaatacatt gcccaacacg acaatgaact taactttatc tccatgctgc cgctggcggg
    41161 ctatgacggc tctttgcagt accgtgcagg tctgcatcag gcgggcgtgg atggcaaagt
    41221 ctcagcgaaa accggttcgt tgcagggggt atataacctg gcgggattca ttaccacagc
    41281 gagcgggcaa cgaatggcgt ttgtgcaata tctttctggc tatgcagtag aaccggcgga
    41341 tcagcgtaat cgccgtattc cgttagtgcg tttcgaaagc cgtttgtata aagatattta
    41401 ccagaacaat tagtcaaaaa gaaaccccgg catatggccg gggtttcaga ttattgacaa
    41461 agtgcgcttt gtttatgccg gatgcggcgt gaacgcctta tccggcctac aaaatcgtgc
    41521 aaattcaata tattgcaatc atcttgtagg cctgataagt gtagcgcatc aggcttattt
    41581 ggcattggtc attagtgatt aacgcttgta aatgaactca acgccttctt cgtcgtcttc
    41641 gtcccagtcg tcatcccagt cctcatcatc ctcttcagca atctcttcaa gctgctggcg
    41701 atgataatca tcccacatga attcgacttt ctctggctgt ttcgcttctt cagcctgcac
    41761 gaccgggttt tcaatgataa aggtcatcac atcccagcag agatctttca cgcccagtcc
    41821 gctcgccgca gagatcagat aatatttatc ttcccagccc agcgcctcag cgatcgcttt
    41881 cgctttctct tcggcttctg ccttatccag cagatcgatc ttgttgaaca ctaaccaacg
    41941 cggtttcgcc gccagatcct ggctgtattt ttccagctcg ctgataataa tacgcgcgtt
    42001 ttcaaccgga tcggtgccgt caatcggatc gatatcgatg aggtgcaaca ggacgcggca
    42061 acgttccagg tgcttcagga agcggatacc cagacctgcg ccttccgcag cgccttcaat
    42121 cagtcctgga atatcggcaa caacgaagct cttttcgttg tccattcgta ccacacccag
    42181 acttggcacc agagtggtaa acggataatc cgccactttc ggtttagccg ccgataccgc
    42241 gcgaataaag gtcgatttcc ccgcgtttgg catccccaac ataccgacgt cagccagcag
    42301 catcagctcc agcagcagct cacgcttatc acccggcgta ccgttggttt tctgccgtgg
    42361 tgtacggtta acggacgatt tgaaacgggt attgcccaga ccgtgccagc cgcccttagc
    42421 aaccagcaga cgctgaccgt gtttagtcat atcgcccatg gtttcaccag taccctggtc
    42481 gattacacgc gtacctaccg gcactttaat cgtgacgtct ttaccgcgct taccggtaca
    42541 gtcgcggctt gcgccgtttt gaccgcgctc tgcacggaaa gatttttcaa aacgataatc
    42601 gataagcgtg ttcaggttct cgtcggcttc catccatacg tcaccaccat caccgccgtc
    42661 gccgccatcc gggccgcctt tcggaatata cttttcgcgg cggaagctca cgcaaccatt
    42721 accgccatca cctgcaacga ccagaatcga tgcttcatca acaaacttca ttttattctc
    42781 cgtaaatcat tcgcctgcgc ggggttgcga taccaccgtt gtatgcttac gtaatccgcc
    42841 ccaaatacga tgaccaatgg cggaatacat cgcgcccgca accacgacaa acgcaccgag
    42901 ataacctaaa aggtttaaca tcggtctggc gaagaaatcg ggccaggcca gtgataaaag
    42961 atctgaaaaa aacagcgtaa acagtggggt gagcgtgatg atcgcgctca cctgcgctgc
    43021 ctgccagcga gccatcgctt ccgccagggc gccatatcct accaatgtat tcagtccgca
    43081 aaaaattaaa catgcgagct gccagtggct aagctgcgct atcactccag gctttgccag
    43141 agggaagagc gcgattgtac ataaagtgta cagtaaaaac agaatctgcg gtgaggccag
    43201 ccgacgcaat aaaacctttt gcgccacgcc ataactcacc caaaccgtcg ccgcaccgac
    43261 cccaaagata actccccagg tgtaatcagt gagctttgta aatatctcga ccagactggt
    43321 gttaaaaaac atcaccaggc cgctcaggag catcaatgcc cctacaacct gagtgctgcg
    43381 cattttctct ttcaggataa atacgctggc aaccatcatg ccaactggcg agagttgccc
    43441 aatcacctgc gaagcggtcg gactcaggta ttgcaaggat gagctgaaca ggatgaagtt
    43501 cccaaacagc ccggcggtcg ccactgccaa caaaatcaac cagcgtggct tacgaaacac
    43561 gcgtaatggc ggcaacctct tcttcaccgc aagaatggca ccaaggccaa tactcgccat
    43621 caagaaacgg taaaacacga ttgtcggagg ttccatcacc tccagcacct gcttcattgc
    43681 gattggcaac gccccccagc aaattgctgt ggtgagcgcc aaaagaatgc caatgcctgc
    43741 ctgctgcttc ataccgtatt ccctgcaaag cgcatttgcg gattaacgtc gcaccatgct
    43801 catttttacc ggttatcgaa tgtaaaaagc cccgcaacgt gttgcggggc tttcatccgt
    43861 taccgggacg cgaaaaactt attcagcttc gatgctgata aatttacggt ttttcgggcc
    43921 tttaacttcg aatttcactt taccgtctgc tttagcaaac agagtgtggt cacgaccgca
    43981 acctacgtta gcgccagcgt ggaatttggt accacgttga cgaacgatga tgctacccgc
    44041 cagaacggat tcgccaccga aacgcttaac gcccaggcgt ttagcttctg aatcgcgacc
    44101 gttacgtgtg gagccgccag cctttttatg tgccatttga aatctctcct caggtcttag
    44161 gcgctgatgc cagtaatttt cacatcagtg aaccactgac gatggccctg ctgcttacga
    44221 tagtgtttac gacgacgaaa cttaacgatt ttaactttct cgccacgacc gtgagcaaca
    44281 acttcagctt tgattacgcc gccatcaacg aaaggaacgc cgattttgac ttcttcaccg
    44341 tttgcgatca tcagcacttc agcgaactca acagtttcgc cagttgcgat gtccagcttt
    44401 tccaggcgaa cggtctgacc ttcgcttact cggtgttgtt taccaccact ttggaaaacc
    44461 gcgtacatat aaaactccgc ttccgcgcac accttttcaa tgattcagag tgcgctataa
    44521 atattcacaa tagggcgcga atattacgca aaacgcacgc ctttgacaag tgctacagtc
    44581 aatacacgaa gaaaaaaaac acaacttgta cggtaacgtt tatctgtgcc attttttcag
    44641 tacaatcacc ctatattcct aaccataaac cctaagttgc ctttgttcac agtaaggtaa
    44701 tcggggcgaa aagcccggct tttgcgatga atttagaaaa aatcaatgag ttaaccgcgc
    44761 aagatatggc gggtgttaat gcggcaatcc ttgagcagct taattccgac gtccaactga
    44821 tcaatcagtt aggctattac atcgtcagcg gcggcggtaa acgtattcgt ccgatgattg
    44881 ctgtactggc tgcacgagct gttggctatg agggaaatgc gcatgtcacc attgctgcct
    44941 tgatcgagtt tatccacacg gcgactctgc tacacgacga cgttgtggat gaatcagata
    45001 tgcgcagggg taaagctacc gccaacgctg catttggcaa tgccgccagc gtgctggtag
    45061 gcgattttat ttatacccgc gcgttccaga tgatgaccag cctcggttcg ctcaaagtgc
    45121 tggaagtcat gtcagaagcc gtaaacgtca tcgcagaagg tgaagttctg caactgatga
    45181 acgttaacga tccggacatc actgaagaaa actacatgcg cgttatctat agcaaaaccg
    45241 cgcgtctgtt tgaggctgcc gcgcagtgtt ccgggattct ggctggctgt acgccggagg
    45301 aggagaaagg cctgcaggat tatgggcgct atctcggcac tgctttccag ttgatcgacg
    45361 atttactcga ttacaatgcc gatggcgaac agttaggtaa aaacgtcggc gacgatctga
    45421 acgaaggtaa accgacgctg ccgctgctgc atgcgatgca tcatggcaca ccagaacagg
    45481 cacagatgat ccgtaccgcc atcgaacagg gtaacggtcg ccatcttctg gaaccggttc
    45541 tggaagcaat gaacgcttgt ggatctcttg aatggacgcg tcagcgcgcc gaggaagaag
    45601 cagacaaagc catcgcagcg ttacaggtgc tcccggacac cccttggcga gaagcactca
    45661 tcggcctcgc gcacatcgct gttcaacgcg atcgttaatc ccctcccctc atcccgcgca
    45721 gcgcgcgggg tggttccagt aaattccata aaaacactta ctcagctcta taacacctga
    45781 aaaacaggct gtgcatcttt atatattctg aatattcaca ctccttacat gaacttttta
    45841 gagcaatcgg ccatcaggag tatagtgatg ctcgacagaa gaagtgttct gaatgaaagc
    45901 gactaactta aggagtgagg aaagatggaa agtaatttca ttgactggca tcccgctgac
    45961 atcattgcgg gtttgcgcaa gaagggaacg tcaatggcgg cggagtctcg cagaaatggt
    46021 ttgagttcct caacgctggc gaatgcatta tcgcgcccat ggccgaaagg agagatgatt
    46081 attgcgaaag ccctgggaac tgacccctgg gttatctggc catcacgcta ccatgatccg
    46141 cagacccatg agtttatcga cagaacgcag ttgatgcgta gctacactaa accgaaaaaa
    46201 tgagtggtct ggcggtagcc ccgcgaacgg ggctgccagc tctcagacga ttattcgcct
    46261 ttcacacgct caatatttgc acctaaagcg cgcagtttgt cttcaatgcg ttcgtagcca
    46321 cgatcgatgt gataaatacg atcaaccacc gtcgtccctt ccgcaataca gccagccagc
    46381 accaggcttg ctgatgcacg cagatcggtt gccataacct gtgcgccaga aagtttttca
    46441 acaccgtgac aaataacggt attgctttcg atttcggcat gcgcgcccat acggctaagc
    46501 tccggcacat gcataaagcg gttttcaaag accgtttcgg tgatgaatcc ggtcccttct
    46561 gccaccaggt tcaacagcgt gaactgggcc tgcatatcgg tcgggaatgc cggatgcggc
    46621 gcggtacgta cgttaacagc cttcggacgt ttgccatgca tatccaggct aatccagtct
    46681 tcgccgactt cgatgtccgc tccagcatca cgcagtttcg ccagcacggc gtccagagta
    46741 tctggctgcg cgttacggca gataattttg ccgcgagaaa tcgccgccgc caccaggaaa
    46801 gtaccggttt cgatacgatc cggtagcacg cgatagacac cgccgcctaa acgttccaca
    46861 ccttcgatga cgatacgatc ggtgccctga ccgctaattt tcgcacccag cgtaatcagg
    46921 aagttcgcgg tatcgacgat ttccggttca cgcgctgcgt tttcaataat cgtggtacct
    46981 tccgccaggg ttgcagcaca catgatggtc accgttgcgc caacgctgac tttatccatc
    47041 acgatatgtg cgcctttcag acgaccatcg acggaagctt taacgtaacc ttcttccagt
    47101 ttgatggtcg cgcctaattg ttcgaggcca gaaatgtgta gatcaaccgg acgcgcaccg
    47161 atcgtacaac cgccaggcag tgaaacttgc ccctgaccaa agcgcgctac cagcggcccc
    47221 agtgcccaga tagaagcacg catggtttta accagatcgt aaggtgcgca gaatacatta
    47281 acgtcgcggg catcaatatg cacagaacca ttacgttcta ctttcgcacc cagctggctt
    47341 agcagcttca ttgatgtatc gacgtctttc agtttcggga cgttctggat ctctaccggt
    47401 tcttccgcca gtagagcggc aaaaaggata ggcagagcag catttttagc gccggaaatt
    47461 gtgacttcgc cctggagctt cgttggcccc tgaacacgaa atttatccat ttagtttgtt
    47521 ctcagttaac aattcatatc cgctaccggc gaatcgccca tagctcaaaa gccgttcagt
    47581 ttgcgatcgc gcgcccactc cgcaggggta tacgctttga tcgacacagc atgaatgcgg
    47641 ttatccgcaa tatattccat cagcgggcca tagaccgtct gctgtttttt aacccgactc
    47701 atgccgtcaa acaactcacc cacggcaata acctgaaagt ggctgccatc gccggaaacg
    47761 tggacttcct ggagggagag agcgttcatc aacacgctct gaatttcatt attttccatg
    47821 ggatcttcaa tcatcagtta ataaaccagc gaaacatctt agagcaaagt tgcgctggca
    47881 taaataagca aaaagcctcg ctgataaatc agacaaggct cgacttgcag gcaggtttgc
    47941 cggacaggcg gttaacgcca tatccggcct gaaaaaattt aacgaggcag aacatcagca
    48001 ggcaaattat acaatttcgc cagggtatac actttgtcgt ttaccccctg aagcgtcaca
    48061 ttgttgccct gctttttcgc cagatcgata agatggagca gcagtgccag tccccccgta
    48121 tccacgcggg agacacggct aagatcgatg cagataatcc ccttcaccgc ttcctcacgc
    48181 atttcccaaa gcggtagcaa aacgtcctga tccagctctc cggataacgc cagcgtgtca
    48241 cccgtctgca tccagctcag tgactcgctc attatttttt ctcttccaga gtgattttct
    48301 gttgagaaat cgatttcagt tgcgctgtca ggccgtcgat acctttggta cgcagcagcg
    48361 ttccccactc gttttgtttg gtggtgatca tactgacgcc ttcagcaatc atgtcgtaag
    48421 cctgccagtt gcccgtctgg gagtttttac gccactggaa gtccagacgc accggcggac
    48481 ggccattcgg gtcaataatg gtaacgcgaa taggcacaat ggttttatcg cccagcggct
    48541 gttctggcgc aatctgatag gtttgaccgt gatacatcgc cagcgcctga ccgtaagcct
    48601 gcttcaggta ctcacggaaa gcggcaaagt aggcttcacg ttgagcaggg gtcgcactct
    48661 tgtaatactg gcccagcacc agcgcaccgg cgtatttcac ctgtacgtat ggcagcagtt
    48721 cctgatcaac aatggtacgc agataatccg ggttggcccg aatttgcggt tgctcattct
    48781 tcaggcgatc gaacgttttc tgcgccgcct cgtccatcag cttatacgga ttggtctggt
    48841 ctgccgcggt tgccgcactc agaggtgcaa tcaccagcaa agcgaccatc attaaacgtt
    48901 taaacatgcg tcggttctcc tgaaattatt tcgttgtacc cacaggttca gtggtttcat
    48961 tattacctgg cgcagcagct ggcgcatcgc cactattctt attgtcatcg cctttactac
    49021 cgtaaaggaa ctgaccaatg agatcttcca gcaccatcgc agacttagtg tcctgaattg
    49081 tatcgccatc cttcaggata gcagtcccca gttccgggtc ttcaaaaccg acgtttaatg
    49141 ccagatattg ttcccccagc aggccggaag tacgaatgct cagcgaactg gtatctggaa
    49201 tgtggttata acgttgttca atttccagcg ttacgcgcgg cagataggtt ttcgggtcca
    49261 gcgtaatatc cgccacccga cccacaacaa cgccaccaat actgaccgga gagcgcgctt
    49321 tcaggccgcc aatgttatcg aacgtcgcat aaagcgtgta ggtcggttca gtacgtatgg
    49381 acgtcacgtt cgccgccttc aggcaaacaa acagcgccgc cagcagtgct gctaataaaa
    49441 agatacccac ccaaatttca ttttttttcg tttgcatgaa ctcaattccc aaacatcaat
    49501 gcggtcagca caaaatccag ccccagaaca gccagagacg agtggacaac ggtgcgagtg
    49561 gttgcccggc taatcccggc agacgtcggg atggcgtcgt agccgttaaa caacgaaatc
    49621 cacgtcaccg tgatggcgaa caccacgctc ttaatcagac agttgaccag atccatacgc
    49681 cagtcgacgg cattttgcat tgccgaccag aagaacccgc tatcaatgcc tttccaactg
    49741 acgccgacca gagatccgcc ccagatcccc acggcgacga aaataaccgt caacagtggt
    49801 aatgaaataa ccccagccca gaaacgggga gaaataaccc gacgcagcgg atccaccgcc
    49861 atcatctcca tactggagag ttgctctgta gcgcgcatca ggccgatttc tgcggttagc
    49921 gccgaaccag cacgcccggc aaacaacaac gcggcaacca ccggccccag ttcacgcagt
    49981 agcgataacg ccaccagcat acccagactg gtttccgcac tataagtggt cagaaccaga
    50041 taaccttgca gccccaacac cattccgatg aacacgccag aaaccacaat aatcagcatc
    50101 gacaggacgc cgacattata gagctggcgc accagcagcg gcgcatgttt gcgaaattcc
    50161 ggtttgccga ccagcgcatt gaataacatt aacccggccc gcccgaacgt tctcagggtt
    50221 ttaatccctt tatgtccgag cgacgccagc gcatttaaca gcatgagtgg cttaactccc
    50281 tggtaaaaga tcagcgtgat aatcgccggc aggatagcgg aacggaacag gcccgtcagc
    50341 tatcccgtcc agaaactgac gtacgcgcgg atcaggattc gcctgcaacg cctgggcact
    50401 gccatgagcg acaatttttt tgtccgccag gatccaggcg tgatccgcaa tacttaacac
    50461 ttccggcaca tcgtgagaaa ccaccacaca agtcacgccc agcgcgctgt tcagctcaga
    50521 aatcagcttc accagtacgc ccatggtgat gggatcttgc ccaacaaaag gttcatcaaa
    50581 catgatgaga tccggctcca gcgcaatcgc acgcgccagc gctgcacgcc gcgccatccc
    50641 accggaaagt tcagaaggca ttagtttagc cgctccacgc agccccacgg cctccagctt
    50701 catcatcacc gtactatgca acaatggcgc gggaagttgg gtatgttcgc gcagtggata
    50761 ggcgacgttg tcaaatacgt tcatatcagt gaacaacgcc ccggactgaa ataacatgct
    50821 catccgtttg cgcactgtat acaggcgcga acgagacatc gccggaatat tctcaccatc
    50881 gaaaaggatc tcaccatgat ctggtgcgat ttgcccgcca atcagacgga gtagcgtcgt
    50941 tttaccgatg cccgatggcc ccatgatcgc cgtgatcttc cctcgcggca cggtcaggga
    51001 aatattatcg aagatgcagc gattgccacg cgtaaaactg acatcgcgca tatcgactaa
    51061 attcgccaca gactgctcca taattcaccc ttcgtcttgc gttgattttc taagcatggc
    51121 gctcaattta accttgaacc caacatattt acagaatatt acccgccgtg gttagcgaaa
    51181 gctggcattt gttttacttt ttagccgcat aaagtcaaaa ttaagaatcc gttacggctt
    51241 tctgaaaatc ttcagcggac cggcgagtat acctgaagaa aggacgttag atgcttttag
    51301 ctacggcact gttaattgtt ggtttacttt tggtcgttta cagtgccgac cgcctggttt
    51361 ttgccgcgtc tattctttgc cgaacctttg gcatcccgcc gctgatcatc ggcatgacgg
    51421 tggtcagtat tggtacatcg ttaccagaaa tcatcgtctc gcttgccgcg tctctgcacg
    51481 aacaacgcga tttagccgtt ggtacagccc tcggctcaaa cattatcaat atattgctga
    51541 tcctcggtct ggctgcgctg gttcgtcctt ttaccgtcca ttctgatgtt ctacgccgtg
    51601 aattaccctt aatgttgttg gtcagcgtgg tggccggttc cgtactctat gacggacaac
    51661 ttagtcgaag cgatggtatc tttctcctct ttctggctgt gctatggctg ctgttcattg
    51721 ttaaacttgc acgtcaggct gaacgtcagg ggactgacag cctgaccaga gagcagcttg
    51781 cagagctgcc gcgtgacggc ggattgcccg tcgcgttttt atggctcggc attgcgctta
    51841 tcatcatgcc agtggccacg cggatggtgg ttgataacgc cacggtgctg gcgaattact
    51901 ttgccatcag cgagttgacg atgggtctga cggcaattgc tatcggaacc agcctgccgg
    51961 aactggcaac cgcaatagcg ggggttcgca aaggtgaaaa cgacattgct gtcggaaata
    52021 tcattggcgc aaacattttt aatattgtca tcgtgttggg tttacccgcg ctgataacgc
    52081 caggagagat tgatccactg gcgtacagtc gtgactacag cgtgatgttg ctggtgagca
    52141 ttatttttgc gttgctgtgc tggcggcgct ccccgcaacc gggccgtggt gtaggggtat
    52201 tattaactgg cggatttatc gtatggctgg cgatgttgta ctggttatcg ccaatactcg
    52261 ttgaataact ggaaacgcat tatgtcgcac gtagagttac aaccgggttt tgactttcag
    52321 caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc tggcggagct tgatcaatac
    52381 atcaatcaga atttcacgct tgcttgtgaa aagatgttct ggtgtaaagg gaaagttgtc
    52441 gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa tggcggcaac gtttgccagc
    52501 accggtacac cttcattttt cgtccatcct ggtgaagccg cgcatggtga tttaggcatg
    52561 gttaccccac aggatgtggt gattgctatc tctaactcag gtgaatccag cgaaatcacg
    52621 gccttaattc cagtgcttaa gcgtcttcac gtaccgttaa tctgcatcac cggtcgcccg
    52681 gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg ttaaagtagc gaaagaagcc
    52741 tgtccgttag ggctggcacc gaccagcagc accaccgcca cgctggttat gggcgatgcc
    52801 ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg aagattttgc gctctcacac
    52861 ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa acgatattat gcatacgggc
    52921 gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg acgcattgct ggaagttacc
    52981 cgcaaaaatc ttggtatgac tgtcatttgc gatgacaata tgatgattga aggcatcttt
    53041 accgacggtg atttacgccg tgtcttcgat atgggcgtgg atgttcgtca gttaagtatt
    53101 gccgatgtga tgacgccggg gggaatacgt gtgcgccctg gcattctggc cgttgaggca
    53161 ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg ttgccgatgg cgaccattta
    53221 ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg tagtgtaaag attcaaggat
    53281 aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg ttacggccct gtcagcgccg
    53341 acgttatggc aaaagcagag aacattcgtc tgctgatcct cgacgttgat ggcgtactgt
    53401 cagatggcct gatttatatg ggcaataacg gcgaagagct gaaagcgttc aatgttcgtg
    53461 acggttatgg cattcgttgt gcgctcacct ctgatattga agtcgctatc attaccgggc
    53521 gaaaggctaa actggtagaa gatcgttgtg ccacattggg gatcactcac ttgtatcagg
    53581 ggcagtcaaa caaactgatc gcctttagcg atttgctgga aaaactggcg attgccccgg
    53641 aaaatgtggc ttatgtcggc gatgatctca tcgactggcc ggtaatggaa aaagtgggtt
    53701 taagcgtcgc cgtggccgat gcgcatccac tgttgatccc gcgcgccgat tacgtgacgc
    53761 gcattgctgg cggtcgtggc gcagtgcgcg aagtttgcga cttattactc ctggcgcagg
    53821 gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa gccagacgtt gggttatcat
    53881 tgtgctatca ctggcggttc tggtgatgat cggcattaat atggccgaaa aagacgatac
    53941 cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa agcgagcata cggacacgct
    54001 cgtctataac ccagaagggg cactaagcta tcgattgatt gctcaacacg ttgaatatta
    54061 ttccgatcag gccgtttcgt ggtttacgca gccggtactt accacgtttg ataaggataa
    54121 aatcccgaca tggtccgtaa aagcagataa agccaagctg accaatgacc ggatgctcta
    54181 tttatatgga cacgttgaag tcaacgcact tgtgccagac tctcaacttc gcagaatcac
    54241 gacggataac gcgcagatca atctggtgac tcaggatgtt acctctgaag acctcgtcac
    54301 gttatacgga acaacattta actccagcgg tctgaaaatg cgcggcaact tacgcagcaa
    54361 gaacgccgag ctgattgaaa aggttagaac atcctatgaa attcaaaaca aacaaactca
    54421 gccttaatct tgtgcttgcc agctcacttc tggccgccag cattccggca tttgccgtaa
    54481 ccggagacac tgatcagccg atccacattg aatcggacca gcaatctctt gatatgcaag
    54541 gcaacgtggt tacctttacc ggtaatgtca tcgtcaccca gggcaccatc aaaattaatg
    54601 ccgacaaagt ggtcgttacc cgtccgggcg gcgaacaagg taaagaagtg attgacggct
    54661 acggtaaacc ggcaacgttc taccagatgc aggacaacgg taaacccgtt gaaggtcacg
    54721 cttcccagat gcactacgaa ctggcaaaag attttgtcgt tctgacgggt aatgcttatc
    54781 tgcagcaggt cgatagcaac attaagggcg ataagatcac ttacctggta aaagagcaga
    54841 aaatgcaggc tttcagcgac aaaggcaagc gcgtaaccac cgttctggtg ccgtcgcagc
    54901 tgcaggataa aaacaacaaa ggccagaccc cggcacagaa gaagggtaat taattcgtta
    54961 tggcaacatt aactgcaaag aaccttgcaa aagcctataa aggccgtcgc gtggtagaag
    55021 acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct gctggggcca aacggtgccg
    55081 gtaagaccac cactttctat atggttgtag gcattgtgcc gcgcgatgcg ggcaacatca
    55141 ttattgatga tgacgatatc agtctgctgc ctctgcatgc acgcgcgcgc cgcggtatcg
    55201 gctatctgcc acaggaagcc tccattttcc gtcgcctcag cgtttacgat aacctgatgg
    55261 cggtactgca aattcgtgac gacttgtctg ctgaacaacg tgaagaccgc gcgaacgagc
    55321 tgatggaaga gtttcacatt gagcacctgc gtgacagcat ggggcagtca ctctccgggg
    55381 gtgaacgtcg ccgtgtagaa attgcccgcg cactggctgc gaatccgaaa tttattctgc
    55441 tcgacgaacc gtttgccggt gttgacccga tctcggttat cgacattaaa cgcatcattg
    55501 agcacctgcg cgacagcggc ctgggcgtgc tgatcactga ccacaacgtg cgtgaaacac
    55561 tggcggtttg tgaacgcgct tatatcgtca gtcaggggca tttgatcgcc cacggcacgc
    55621 ctacagaaat cttacaagac gaacacgtta agcgtgtata ccttggggaa gacttcagac
    55681 tctgataggg tagaagtttg cgacgtttta gcaggagagt acgattctga acatgaagca
    55741 aggtttgcaa ctcaggctta gccaacaact ggcgatgacg ccacagctcc aacaggcaat
    55801 tcgtctgttg cagttgtcga cgctggaact tcagcaggag ctacagcagg cgctggagag
    55861 taatccgctg cttgagcaaa tcgacactca tgaagaaatc gacacccgcg aaacgcaaga
    55921 cagtgaaacg ctggacaccg ccgacgcgct cgaacaaaaa gagatgccgg aagagctgcc
    55981 gctcgatgcc agttgggaca ccatttacac cgctggtaca ccatccggca ccagcggtga
    56041 ctacattgac gatgagctgc cggtctatca gggcgaaacg acgcagacct tgcaggatta
    56101 cctgatgtgg caggtcgagc tgacaccgtt ttccgacact gaccgcgcta ttgctacctc
    56161 tatcgtcgat gccgttgatg acaccggtta tctgactgtc ccgctggaag atattctcga
    56221 aagtatgggc gatgaagaga tcgacatcga cgaggttgaa gccgtcctta agcggatcca
    56281 acggtttgat ccggtcggtg tagcggcaaa agatctgcgt gactgcctgc taatccaact
    56341 ctcccaattc gataaaacca cgccgtggct ggaagaggcc agattgatca ttagcgatca
    56401 tctcgatctg ttagccaatc acgacttccg cactttaatg cgcgtcacgc gtctgaaaga
    56461 agatgtgctg aaagaagccg tcaatctgat ccagtcgctc gatccgcgcc ccgggcagtc
    56521 gatccagact ggcgaacctg agtatgtcat tccagatgtg ctggtgcgta agcataacgg
    56581 tcactggaca gtagaactca acagtgacag cattccgcgt ctgcaaatca accagcacta
    56641 cgcctcgatg tgcaataacg ctcgcaatga tggtgacagc cagtttatcc gcagcaatct
    56701 gcaggatgcc aaatggttga tcaagagtct ggaaagccgt aacgatacgc tactgcgcgt
    56761 gagtcgctgt atcgttgaac agcagcaagc cttctttgag caaggcgaag aatatatgaa
    56821 accgatggta ctggccgata tcgcccaggc tgtcgaaatg catgaatcga cgatatctcg
    56881 cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt tttgaactga agtatttctt
    56941 ttccagtcac gtcaataccg agggcggcgg cgaagcttcc tccacggcga ttcgtgcgct
    57001 ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg ttgagcgaca gcaagttaac
    57061 ctctttgctg tcggaacaag gtatcatggt ggcacgccgc actgttgcga agtaccgaga
    57121 gtctttatcc attccgccgt caaaccagcg taaacagctc gtttgaccca accgataagg
    57181 aagacactat gcagctcaac attaccggaa ataacgtcga gatcaccgag gcactgcgcg
    57241 aatttgttac agccaaattt gccaaacttg agcaatattt tgaccgaatc aaccaggtct
    57301 atgttgttct gaaagtggag aaagtcaccc acacctcaga tgcaacactg catgtaaacg
    57361 gtggtgaaat tcatgccagc gcggaaggtc aggacatgta cgctgccatt gatggcttaa
    57421 ttgataagct ggcacgccag ttgaccaaac ataaagataa actgaaacaa cactaattgt
    57481 ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca caacgggcca tttgtacggt
    57541 taatgctccg agcctgttcc actgtttgag tgggcaggtt cttaggtgaa attatgacaa
    57601 ataatgatac aactctacag cttagcagtg ttcttaacag ggaatgtacg cgaagccgcg
    57661 tccactgtca gagcaaaaaa cgcgccctgg aaatcatcag cgagctggcg gcgaaacaac
    57721 ttagcctgcc gcctcaggtg gtttttgaag ctatcctgac gcgggaaaaa atgggcagta
    57781 ccggtatcgg caatggtatt gccattccgc atggcaaact ggaagaagat actctgcgcg
    57841 ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt cgatgccatc gacaaccaac
    57901 cggtggactt actttttgcc ctgctggtgc cggcagacca aactaaaacg cacctgcata
    57961 ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat ctgccgccgt ttacgcgcag
    58021 cccagagcga tgaagagctg tatcaaatca ttacggatac cgaaggtact ccggatgaag
    58081 cgtagttatt cggtaatgtc tcttttagac gttgtgagga gaaacagtac atggtactga
    58141 tgatcgtcag cggacgttca ggttcaggta aatctgtcgc cctgcgtgcg ctggaagata
    58201 tgggttttta ctgcgtggat aaccttcccg tagtgttgtt acccgatctg gctcgaactc
    58261 tggccgatcg cgagatttct gccgccgtca gcattgatgt tcgtaatatg ccggagtcac
    58321 cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc tttctcaccg caactactgt
    58381 tcctggatgc cgaccgtaat accttaattc gtcgttacag tgacacgcgc cgactgcatc
    58441 cgctttccag caaaaacctg tcgctggaaa gtgctatcga caaagaaagc gatttgctgg
    58501 agcctctgcg ttcgcgagcg gatctgattg tcgatacctc agaaatgtcc gttcacgagc
    58561 tggctgaaat gctgcgtacc cgtctgctgg gtaaacgtga acgcgaactg accatggtct
    58621 ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc agattacgtc tttgacgtgc
    58681 gcttcttgcc gaacccgcac tgggatccga aactgcgtcc aatgacaggt cttgataaac
    58741 ctgtcgccgc gttcctcgac cgccacacag aagtacacaa ttttatctac cagacgcgaa
    58801 gctatcttga actatggtta cctatgctgg aaaccaacaa ccgtagctac ttgacggtcg
    58861 ccattggttg taccggcgga aagcaccgtt cggtgtatat tgcagagcaa ctggcagact
    58921 acttccgctc gcgcggtaaa aacgtccagt cacgccatcg tacgctggaa aaacgtaaac
    58981 catgaccgtc aagcaaactg ttgaaatcac aaacaagctg ggcatgcatg cccggcctgc
    59041 aatgaagctg tttgaattaa tgcagggttt tgacgctgaa gtgctgttac gtaatgacga
    59101 aggcaccgag gccgaagcca acagcgttat tgcgctgctg atgttggatt ctgccaaagg
    59161 acggcagatt gaagttgaag cgaccggccc acaggaagag gaagcactgg ccgccgttat
    59221 cgccctcttt aactctggtt ttgatgaaga ttaatcttca tcactttgac atacaactcc
    59281 cttcaaactc cccccgacaa taagaaaatc acgtactgaa atcgttctca atcaacgtca
    59341 tttgtacatt ttgtgcgctt ttcactttca gaagaacctt aagaaaacct taagaggcat
    59401 tgtttaggtt ttgtttaagt taatcgacca tactggagat cgtcagaaaa tatttccagg
    59461 agatggcatg attcgcttat ctgaacaaag tcccctgggc accggaagac atcggaaatg
    59521 ttatgcgcat ccggaagatg cccaacgctg tatcaagatt gtctaccatc gtggcgatgg
    59581 tggcgataaa gagatccgcc gtgagttaaa gtactacgcg catcttggtc gccggttaaa
    59641 agactggagt ggaataccgc gctatcacgg taccgttgag acggattgcg gaaccggtta
    59701 tgtctacgat gtgattgcag attttgacgg caaaccctcc attacgctga ccgaatttgc
    59761 tgaacaatgc cgttacgaag aagacattgc acaactgcgc cagctactga aacagctgaa
    59821 gcgttatttg caggataacc gtatcgtgac gatgtcgctg aagccgcaga atatcctctg
    59881 tcaccgcatc agtgagtcag aggttatccc ggtcgtctgt gacaacattg gtgaaagcac
    59941 attgattcca ctggcgacct ggtcgaagtg gtgttgtctg cgtaagcaag agcggttatg
    60001 gaaacgattt atcgcccaac cggcgctggc tattgccctg cagaaagatc tgcaacctcg
    60061 tgagagcaaa acgctggccc tcacttcgcg cgaagcttaa tccagctggt gttgttgcat
    60121 aaacggctca ccgcctaact gatacatctg ccgtaaaatc cacgcctgac ggctacgcac
    60181 gtagcccgat ggcgaggaga ctttgaaacg aagtggatta ggtaatacag ctgccagtaa
    60241 tgcagcttcc gaccgggtaa gtttgctcgc gggtttgtgg aaataacgtt gtgccgcagc
    60301 ttcgacgcca aacacaccgt cgccaaattc ggcgatattc aggtaaaccg tcaggatacg
    60361 ctttttgctc cagactgttt ctatccccag cgttaatcca gcttccagcc cttttcgtac
    60421 ccagctacgc ccatcccata aaaagagatt tttggccgtc tgctgagaaa tcgttgaagc
    60481 accgcgaata cggttttcat tgcgctcgtt gtgcgccagg gctttctcaa tggaagcgac
    60541 atcaaagccc cagtgctcag gaaatttctg atcttctgcg gcaatcaccg ccagtcccat
    60601 ccacggcgag atttgatcca tactgaccca gtcagaatgt gccacgtaac gaaaattgcc
    60661 atgcagccag gcgctgacct gtcgctcgac cattaccgct gagaagggaa caggcgcaac
    60721 actaaacaac gcgatgcccc cgccccagaa aacggcgagg acaaccatta accgcaaaag
    60781 aaaacggcga acgaaactaa acaccgttaa gcggctttta ctcattcagc cagaaccagc
    60841 acgcgggaaa ccagcttatc aatgccgctc gccgcttctg caatgttctg cgccagcata
    60901 tatgctgggg tggtgacaat cttattgtct tcatcaacca cgatatcatc gacaggacag
    60961 ggcacatgct ccgcgcccat ctcttccagc acttctgcgg tatcgatatc agtaccgatg
    61021 gtcaaacgca gcgggaaatc gaaaattttc ggcagcatcg ccggggcaat acacataaaa
    61081 ccaagcggtt ttccggcctg atgcatcgct tgtgccagcg cctttaattc acggtcaacg
    61141 gtgcattcgc taccaagagt ggcaaaattg cttaaattct tcgccgcacc aaaccccccc
    61201 ggcacaatca acgcatccag ttcagcggca tcggcctggg ccagaggacg gatttcaccg
    61261 cgcgttattc gtgccgcttc aatcagcacg ttgcgcgttt ccgtcatcgc ttcgccagtt
    61321 aaatggttga taacatcaac ctgctgctta tccggtgcaa agcagaccgc ctgcgcaccg
    61381 ctgcgtgaaa tagctaacaa cgtcaacacc gcttcatgaa tttcagaacc gtcatagacg
    61441 ccgcatccgc tcagaattac gccaattttc ttcattgtga tcatcctttc gcaattgact
    61501 gaaacacatt aattttttta ataaaaatgg tacgcatcac acatttaact gattcatgta
    61561 acaaatcatt taagttttgc tatcttaact gcgtgcggcc tgaaaaacag tgctgtgccc
    61621 ttgtaactca tcataataat ttacggcgca gccaagattt ccctggtgtt ggcgcagtat
    61681 tcgcgcaccc cggtctagcc ggggtcattt tttagtggct tttgccaccc acgctttcag
    61741 cacttctacg tcgtgacgcc actcttcttt catctcttca atccattcac cgacgttatc
    61801 ttcccaggca ggaaggtccg gagactgaat ttgctgaccc agctgttgca gatggcgtaa
    61861 ccccactgac cccgccgcac ctttaatttt atgtccttcc tctacaatgc cttttttatc
    61921 ctgcgccgtc agattcgact ccagcacgct gacatagccc ggcatcatct tctcaaacac
    61981 cgctaacccg tcggtgatca gcttcggtcc tacaagttcg agatactgtt ccagcatggg
    62041 aatatcgagc aatgcttctg atttactgtt ctcttctgtc gtcaccgtac tctcctcatc
    62101 atcctgggta tcccagaatt tcttgatcat cgcggttagc gccggaaccg aaagcggctt
    62161 actcagcaca tcatccattc cagcattgag gtactcttgt ttgtctttca gcacgttagc
    62221 ggttaaggcc accagcggcg gtaaatcctc gcgcggataa cgcttcgtca gttcacgaga
    62281 gatatccagc ccggtcatat ctggcaactg aatatccagc aacaccaggt cgtattcgcc
    62341 cggtttaaac atctccagcg ccgccttgcc ggtcatggcg acatcaacgc tgttacctaa
    62401 tttttccagc acagaacgcg caacaatcac gttcagttca atgtcttcca ccagcagcac
    62461 attcagcgcc ggtaaaggca tatcgtcttc atcaaacgca tcatcgacct cttctgctac
    62521 cgacggtgcg tggatcgtca acgtaaaggt tgaacctttg ccctgttcgc tggtaaccgt
    62581 aatatcgccg cccatatttt tcgccagacg acgagaaacg gccagaccaa taccggtgcc
    62641 ggtggcaggt ttaccgccat gactgtcttt cacctggtaa tacatggcga aaattttatc
    62701 cagctcatcc tgcggaatgc cgataccaga gtcttccact tcaaaatgca gcatatcgcc
    62761 ttcatcgtag cgcacgcgca cggtaacctg gccttgctgg gtgaatttga cggcgttact
    62821 gatgaggttc cacaggatct gccgtaaacg cgtcccgtcg gtaatgacct gatgcggtaa
    62881 tggcagcgtc ggctccaggt taaagcgcaa tcctttttgt tgcgcctgca atgcggagag
    62941 attttccaga tcggcaagga agctggtgaa atcaaccggt tgattatcaa gctggacctt
    63001 gcgccgttcc atcttatcca tgtcgataat atcgttaaag atattcccca gcgtgacggc
    63061 cgaaacatgg atggtcttga gatatttttc ctgctcggcg gtgagttcgg tatccagcag
    63121 aatacggctc aggccgacga taccgttcag cggtgtacgc aattcgtgac tgatggtgga
    63181 gataaacgtc gttttgtcgc ggctggcccg ttcaagcgca tcctgatacc gcttacgctc
    63241 ggtaatgtcg cgaccaaagc ccatcaaacc gtgacgttta cccacgcggt cgtagtacgg
    63301 cactttacgg atttcaaagc aggctttgcg cccgtccggg taatccagcc actgttcata
    63361 ggtcagtgac acattatgac ggaacacttt ttcatcggtt tcaatgactt ttgcggcggc
    63421 ttccggtgag taaacatcag caggtttcag gtgaaccagt tgtttttcgc tttttccggt
    63481 cagcagctcc atcgcgcggt tacagccgga aaactcttta tcttcgttac gataaaaaac
    63541 caggtcgggt gaagcatcaa ggaaggaacg taagaatgag gattgctgct cgagctgaat
    63601 ttgtgtctct tcgcgctctt tgatttcaat tttcagttgg ccgaaggttt cctgcagttc
    63661 tgcttccgct ttttcacgaa cggcgatttc ctgatttagc tgggcaatat tatcttttaa
    63721 ctgaacgttg aggctcaaat cgcgctcgcg catctcctcc agtttttgca ccagccgtga
    63781 cagacgttgt cgtgactcct ccagttgctc gacgaccacc gatagaaagt agaccgccca
    63841 cggcgtaatc agcaaaccaa agaagataga acgaataaca tcaatgcttt cgacctgacc
    63901 atgcagcacc atggttaccg ccatttgtac cacaatggca agaacgacga gggccagcgc
    63961 cagcaacatt gagaagcgca ccagacctaa cttcatcatc aggtcaacat aatactgcgc
    64021 cagcagacga atttgcttca ttagggaatt ccttcacgac aacctgcccc aataataccc
    64081 aattctgaca gttacgttat aaattgtgtg agaaatgcgg aattcccctc aacgacctac
    64141 tccgttggag gtatccacgg acgttccagc gccgatccct gtacgccatg ttcattcaga
    64201 tagcgatcca gctcgaccat tcccgtccat cgattttcgc accacagagg agcaagcagc
    64261 gtcgggcgac gggcgctggc ggaaatgcgg tggtagatca cttccggtgg cgtatggcga
    64321 atcatttctc cggcagtgag cgtgtaatcc tccagttcaa taccgttcaa acgccccgct
    64381 tcccaggctt tcgccataat gctgcctttc acaatatgca gcggatgcag ctttatgccg
    64441 tccacgccgg tttcaaccac tcgctccagc gtttgcaaac attcagcctg cccttcgcca
    64501 ggcaggccga caattaagtg ggaacacacc ttcagcccgc gcagacgtgc cagctgggtt
    64561 gtacgctgat aacaggcaaa atcatggccg cggttgatgc gatgcagtgt tttgtcgtgg
    64621 gcggtttgta gccccagctc cagccacact tcgtaaccct ggtccttata ttcgcaaagc
    64681 agatccagca ccgcatccgg cacgcagtcc gggcgggtac caacacacaa accgacaata
    64741 ttggcctggc tcaccgcctg ctgatacata gaacgcagca cctgaacttc cgcaaaggtg
    64801 ctggtatacg cctgaaagta ggccagatag cgtttagcgc ggttaactaa attcgcctgg
    64861 tgcgccagtt gctcggcaat ggaacgatgc tgctgcgctt catcggcaaa cgaggcaaca
    64921 ttacagaatg tgcagccacc acgcccgatg gtaccgtcac ggttagggca gctaaaaccg
    64981 ccgtgtagcg tcagcttatg caccttttgc ccataacgac gggtgagatc accaccaaac
    65041 atattgacta atttctgtaa ctgcataatc tgatagaccg cgccttgaaa agaggccaaa
    65101 gcctgccatt tttagctcaa ttcggcgatg acctggatca atcgtcccgg cccgctttta
    65161 tcaactgcat aatcaatcaa aattaccgaa atttcatgca taatcacata aatcactttt
    65221 gcttatcttg tgtcagattt ttttatctcc ttatggattt taggcaaaaa cagtagcatg
    65281 aaacgtcatt accaattaag gcagtataaa atgctggttt tctcgtcagt tcaaggcagg
    65341 ataagggtta acacgccttt atgacagtca ggaat
//
LOCUS       DAFNBJ010000006        62965 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000006 DAFNBJ010000000
VERSION     DAFNBJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.006"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            11..1777
                     /gene="adeD"
                     /locus_tag="L2X49_000399"
     CDS             11..1777
                     /gene="adeD"
                     /locus_tag="L2X49_000399"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBN4795543.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1822..3213)
                     /gene="uhpT"
                     /locus_tag="L2X49_000400"
     CDS             complement(1822..3213)
                     /gene="uhpT"
                     /locus_tag="L2X49_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBN4795544.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3351..4670)
                     /gene="uhpC"
                     /locus_tag="L2X49_000401"
     CDS             complement(3351..4670)
                     /gene="uhpC"
                     /locus_tag="L2X49_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBN4795545.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4680..6182)
                     /gene="uhpB"
                     /locus_tag="L2X49_000402"
     CDS             complement(4680..6182)
                     /gene="uhpB"
                     /locus_tag="L2X49_000402"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBN4795546.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6182..6772)
                     /gene="uhpA"
                     /locus_tag="L2X49_000403"
     CDS             complement(6182..6772)
                     /gene="uhpA"
                     /locus_tag="L2X49_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBN4795547.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6847..7137)
                     /gene="ilvN"
                     /locus_tag="L2X49_000404"
     CDS             complement(6847..7137)
                     /gene="ilvN"
                     /locus_tag="L2X49_000404"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBN4795548.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7141..8829)
                     /gene="ilvB"
                     /locus_tag="L2X49_000405"
     CDS             complement(7141..8829)
                     /gene="ilvB"
                     /locus_tag="L2X49_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBN4795549.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8935..9033)
                     /gene="ivbL"
                     /locus_tag="L2X49_000406"
     CDS             complement(8935..9033)
                     /gene="ivbL"
                     /locus_tag="L2X49_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBN4795550.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9598..9687
                     /gene="tisB"
                     /locus_tag="L2X49_000407"
     CDS             9598..9687
                     /gene="tisB"
                     /locus_tag="L2X49_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBN4795551.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10106..11290
                     /gene="emrD"
                     /locus_tag="L2X49_000408"
     CDS             10106..11290
                     /gene="emrD"
                     /locus_tag="L2X49_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBN4795552.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11298..11795)
                     /locus_tag="L2X49_000409"
     CDS             complement(11298..11795)
                     /locus_tag="L2X49_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBN4795553.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11792..12154)
                     /locus_tag="L2X49_000410"
     CDS             complement(11792..12154)
                     /locus_tag="L2X49_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBN4795554.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12144..12491)
                     /locus_tag="L2X49_000411"
     CDS             complement(12144..12491)
                     /locus_tag="L2X49_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBN4795555.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12601..13050
                     /locus_tag="L2X49_000412"
     CDS             12601..13050
                     /locus_tag="L2X49_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795556.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13097..14590)
                     /locus_tag="L2X49_000413"
     CDS             complement(13097..14590)
                     /locus_tag="L2X49_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBN4795557.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(14587..16302)
                     /locus_tag="L2X49_000414"
     CDS             complement(14587..16302)
                     /locus_tag="L2X49_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBN4795558.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            16469..17362
                     /locus_tag="L2X49_000415"
     CDS             16469..17362
                     /locus_tag="L2X49_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBN4795559.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17359..17535)
                     /locus_tag="L2X49_000416"
                     /pseudo
     CDS             complement(17359..17535)
                     /locus_tag="L2X49_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(17535..18173)
                     /locus_tag="L2X49_000417"
     CDS             complement(17535..18173)
                     /locus_tag="L2X49_000417"
                     /inference="COORDINATES: protein motif:HMM:NF014148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795560.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQERFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHWWSAISRSFRKG"
     gene            complement(18173..19789)
                     /locus_tag="L2X49_000418"
     CDS             complement(18173..19789)
                     /locus_tag="L2X49_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN4795561.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            20085..20801
                     /locus_tag="L2X49_000419"
     CDS             20085..20801
                     /locus_tag="L2X49_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN4795562.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(20798..22459)
                     /locus_tag="L2X49_000420"
     CDS             complement(20798..22459)
                     /locus_tag="L2X49_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBN4795563.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGLPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22656..23084)
                     /gene="ibpB"
                     /locus_tag="L2X49_000421"
     CDS             complement(22656..23084)
                     /gene="ibpB"
                     /locus_tag="L2X49_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBN4795564.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23196..23609)
                     /gene="ibpA"
                     /locus_tag="L2X49_000422"
     CDS             complement(23196..23609)
                     /gene="ibpA"
                     /locus_tag="L2X49_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBN4795565.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23603..23675)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23915..24247
                     /locus_tag="L2X49_000423"
     CDS             23915..24247
                     /locus_tag="L2X49_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBN4795566.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24249..25463)
                     /locus_tag="L2X49_000424"
     CDS             complement(24249..25463)
                     /locus_tag="L2X49_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBN4795567.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            25564..26628
                     /gene="cbrA"
                     /locus_tag="L2X49_000425"
     CDS             25564..26628
                     /gene="cbrA"
                     /locus_tag="L2X49_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBN4795568.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFVEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(26625..27962)
                     /locus_tag="L2X49_000426"
                     /pseudo
     CDS             complement(26625..27962)
                     /locus_tag="L2X49_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(28037..29185)
                     /gene="dgoD"
                     /locus_tag="L2X49_000427"
     CDS             complement(28037..29185)
                     /gene="dgoD"
                     /locus_tag="L2X49_000427"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBN4795569.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(29182..29799)
                     /gene="dgoA"
                     /locus_tag="L2X49_000428"
     CDS             complement(29182..29799)
                     /gene="dgoA"
                     /locus_tag="L2X49_000428"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBN4795570.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29783..30661)
                     /gene="dgoK"
                     /locus_tag="L2X49_000429"
     CDS             complement(29783..30661)
                     /gene="dgoK"
                     /locus_tag="L2X49_000429"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBN4795571.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     VELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30658..31347)
                     /gene="dgoR"
                     /locus_tag="L2X49_000430"
     CDS             complement(30658..31347)
                     /gene="dgoR"
                     /locus_tag="L2X49_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBN4795572.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31625..32281
                     /locus_tag="L2X49_000431"
     CDS             31625..32281
                     /locus_tag="L2X49_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795573.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32327..33139)
                     /gene="yidA"
                     /locus_tag="L2X49_000432"
     CDS             complement(32327..33139)
                     /gene="yidA"
                     /locus_tag="L2X49_000432"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBN4795574.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33254..33652)
                     /locus_tag="L2X49_000433"
     CDS             complement(33254..33652)
                     /locus_tag="L2X49_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBN4795575.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(33892..36306)
                     /gene="gyrB"
                     /locus_tag="L2X49_000434"
     CDS             complement(33892..36306)
                     /gene="gyrB"
                     /locus_tag="L2X49_000434"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBN4795576.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36335..37408)
                     /gene="recF"
                     /locus_tag="L2X49_000435"
     CDS             complement(36335..37408)
                     /gene="recF"
                     /locus_tag="L2X49_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBN4795577.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37408..38508)
                     /gene="dnaN"
                     /locus_tag="L2X49_000436"
     CDS             complement(37408..38508)
                     /gene="dnaN"
                     /locus_tag="L2X49_000436"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBN4795578.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38513..39916)
                     /gene="dnaA"
                     /locus_tag="L2X49_000437"
     CDS             complement(38513..39916)
                     /gene="dnaA"
                     /locus_tag="L2X49_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBN4795579.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40213..40293
                     /locus_tag="L2X49_000438"
     CDS             40213..40293
                     /locus_tag="L2X49_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795580.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40523..40663
                     /gene="rpmH"
                     /locus_tag="L2X49_000439"
     CDS             40523..40663
                     /gene="rpmH"
                     /locus_tag="L2X49_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBN4795581.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40680..41039
                     /gene="rnpA"
                     /locus_tag="L2X49_000440"
     CDS             40680..41039
                     /gene="rnpA"
                     /locus_tag="L2X49_000440"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBN4795582.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            41003..41260
                     /gene="yidD"
                     /locus_tag="L2X49_000441"
     CDS             41003..41260
                     /gene="yidD"
                     /locus_tag="L2X49_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBN4795583.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41263..42909
                     /gene="yidC"
                     /locus_tag="L2X49_000442"
     CDS             41263..42909
                     /gene="yidC"
                     /locus_tag="L2X49_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBN4795584.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            43015..44379
                     /gene="mnmE"
                     /locus_tag="L2X49_000443"
     CDS             43015..44379
                     /gene="mnmE"
                     /locus_tag="L2X49_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBN4795585.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            44622..44696
                     /gene="tnaC"
                     /locus_tag="L2X49_000444"
     CDS             44622..44696
                     /gene="tnaC"
                     /locus_tag="L2X49_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBN4795586.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            44917..46332
                     /gene="tnaA"
                     /locus_tag="L2X49_000445"
     CDS             44917..46332
                     /gene="tnaA"
                     /locus_tag="L2X49_000445"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBN4795587.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            46423..47670
                     /gene="tnaB"
                     /locus_tag="L2X49_000446"
     CDS             46423..47670
                     /gene="tnaB"
                     /locus_tag="L2X49_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBN4795588.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYPILRDATSTTAGTSYFQYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            47803..48978
                     /gene="mdtL"
                     /locus_tag="L2X49_000447"
     CDS             47803..48978
                     /gene="mdtL"
                     /locus_tag="L2X49_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBN4795589.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            48953..49912
                     /gene="yidZ"
                     /locus_tag="L2X49_000448"
     CDS             48953..49912
                     /gene="yidZ"
                     /locus_tag="L2X49_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBN4795590.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWSLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            50069..50818
                     /locus_tag="L2X49_000449"
     CDS             50069..50818
                     /locus_tag="L2X49_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBN4795591.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPLHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            50840..51406
                     /gene="yieF"
                     /locus_tag="L2X49_000450"
                     /gene_synonym="chrR"
     CDS             50840..51406
                     /gene="yieF"
                     /locus_tag="L2X49_000450"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBN4795592.1"
                     /translation="MSEKLKVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(51460..52797)
                     /gene="adeP"
                     /locus_tag="L2X49_000451"
     CDS             complement(51460..52797)
                     /gene="adeP"
                     /locus_tag="L2X49_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBN4795593.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
     gene            52964..53629
                     /gene="yieH"
                     /locus_tag="L2X49_000452"
     CDS             52964..53629
                     /gene="yieH"
                     /locus_tag="L2X49_000452"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBN4795594.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            53696..54169
                     /locus_tag="L2X49_000453"
     CDS             53696..54169
                     /locus_tag="L2X49_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795595.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            54218..54805
                     /locus_tag="L2X49_000454"
     CDS             54218..54805
                     /locus_tag="L2X49_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBN4795596.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKRGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(54867..55589)
                     /locus_tag="L2X49_000455"
     CDS             complement(54867..55589)
                     /locus_tag="L2X49_000455"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBN4795597.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(55604..56773)
                     /locus_tag="L2X49_000456"
     CDS             complement(55604..56773)
                     /locus_tag="L2X49_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBN4795598.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(56800..58416)
                     /gene="bglH"
                     /locus_tag="L2X49_000457"
     CDS             complement(56800..58416)
                     /gene="bglH"
                     /locus_tag="L2X49_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBN4795599.1"
                     /translation="MFRRNLITSAVLLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(58502..59896)
                     /gene="bglB"
                     /locus_tag="L2X49_000458"
     CDS             complement(58502..59896)
                     /gene="bglB"
                     /locus_tag="L2X49_000458"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBN4795600.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(59915..61792)
                     /gene="bglF"
                     /locus_tag="L2X49_000459"
     CDS             complement(59915..61792)
                     /gene="bglF"
                     /locus_tag="L2X49_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBN4795601.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTSLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(61924..62760)
                     /gene="bglG"
                     /locus_tag="L2X49_000460"
     CDS             complement(61924..62760)
                     /gene="bglG"
                     /locus_tag="L2X49_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBN4795602.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILG
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVHK
                     EH"
BASE COUNT        15426 a        16655 c        15776 g        15108 t
ORIGIN      
        1 tgagaaaaat atgaataatt ctattaacca taaatttcat cacattagcc gggctgaata
       61 ccaggaattg ttagccgttt cccgtggcga cgctgttgcc gattatatta ttgataatgt
      121 ctctattctc gacctgatca atggcggaga aatttccggc ccaattgtga ttaaaggacg
      181 ttacattgcc ggtgttggcg cagaatacgc tgatgctccg gctttgcagc ggattgatgc
      241 tcgcggcgca acggcggtgc cagggtttat tgatgctcac ctgcatattg aatccagcat
      301 gatgacgccg gtcacttttg aaaccgctac cctgccgcgc ggcctgacga ccgttatttg
      361 cgaccctcat gaaatcgtca acgtgatggg agaagccgga ttcgcctggt ttgcccgctg
      421 tgccgaacag gcaaggcaaa accagtactt acaggtcagc tcttgcgtac ccgccctgga
      481 aggctgcgat gttaacggtg ccagttttac ccttgaacag atgctcgcct ggcgggacca
      541 tccgcaggtt accggccttg cagaaatgat ggactaccct ggcgtaatta gcgggcagaa
      601 tgcgctgctc gataaactgg atgcatttcg ccacctgacg ctggacggtc actgcccggg
      661 tttgggtggt aaagaactta acgcctatat tactgcgggt attgaaaact gccacgaaag
      721 ttatcagctg gaagaaggac gccggaaatt acaactcggc atgtcgttga tgatccgcga
      781 agggtccgct gcccgcaatc tcaacgcgct ggcaccgttg atcaacgaat ttaacagccc
      841 gcaatgcatg ctctgtaccg atgaccgtaa cccgtgggag atcgcccatg aaggacacat
      901 cgatgcctta attcgccgcc tgatcgaaca acacaatgtg ccgctgcatg tggcatatcg
      961 cgtcgccagc tggtcgacgg cgcgccactt tggtctgaat cacctcggct tactggcacc
     1021 cggcaagcag gccgatatcg tcctgttgag cgatgcgcgt aaggtcacgg tgcagcaggt
     1081 actagtgaaa ggcgagccga ttgatgcgca aaccttacag gcggaagagt cggcgagact
     1141 ggcacaatcc gctccgccat atggcaacac cattgcccgc cagccagttt ccgccagcga
     1201 ctttgccctg caatttacgc ccggaaaacg ctatcgggtc attgacgtca tccataacga
     1261 attgattacg cactcccact ccagcgtcta cagcgaaaat ggttttgatc gcgatgatgt
     1321 gagctttatt gccgtacttg agcgttacgg gcaacggctg gctccggctt gtggtttgct
     1381 tggcggcttt ggactgaatg aaggtgcgct ggctgcgacg gtcagccatg acagccataa
     1441 tattgtggtg atcggtcgca gtgccgaaga gatggcgctg gcggtcaatc aggtgattca
     1501 ggatggcggc gggctgtgcg tggtacgtaa cggccaggtc caaagtcatc tgccgttgcc
     1561 cattgccggc ctgatgagca ccgacacggc gcagtcgctg gcggaacaga ttgacgcctt
     1621 gaaagccgcc gcccgtgaat gcggtccgtt acccgatgag ccgtttattc agatggcgtt
     1681 tctttctctg ccagtgatcc ccgcgctaaa actaaccagt caggggctat ttgatggcga
     1741 gaagtttgcc ttcactacgc tggaagtcac ggaataataa aaaagcccgg cgtcatgccg
     1801 ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt tctcgcggcg
     1861 gattttgcgt tcttccatca ccgccactat cgccatcagg cagatacaac caatcgcggc
     1921 gatatcaagt gcggcgaatg tgcctgccca gccggtaagg ccgaataccg gcgtcccatc
     1981 ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag caaaggtgcc
     2041 tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag cagccacacc
     2101 aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag ccagatagat
     2161 atattcgttg ctggcatgtt gatacacacc gagcgtggcg ataatcagcg ccagcgcgat
     2221 gcaggccacc aggccacggc gaccgttcgc caggtcagag agccagcccc acagcagcgt
     2281 accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg ctttagagag
     2341 tttcagttcc tggaacgcgt atacggttga ccactggtcg ataccaatac gtaccacata
     2401 gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca catactcaac
     2461 aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt cgctgatctc
     2521 ctcgccgaac agttcttcag ctttgccgag gccataagat tccggggagt cgctaccgta
     2581 acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa acatgccgat
     2641 gacatggcca tcgaacaggt aatttgcccc gaacagcgcc acacctgctg cgcctgcacc
     2701 gccaaggttg tgagaaatat tccagaaacc gaggaatgtc ccgcgtttac gacgcggcgt
     2761 ccatttggtg atggtggagt aactgcacga accgccggta ctctggaaaa agccgcttaa
     2821 ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac tggcactgaa
     2881 gcccagcata caaatggcag acaggataag cataaacggc aggaattgtt tggtgttttt
     2941 gccgtcggcg tagtaggaaa ccagcgtttt acccacgcca taagtgatgg agaaacccag
     3001 gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gaaatcatgt cgttctgcgc
     3061 gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga ccaccaggta
     3121 ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa ggtccagggt
     3181 cggcttgcga acctggttta agaaagccag catgggttac tcctgaaatg aatacctgcg
     3241 tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc caggcaaaac
     3301 ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca tcacgcttcg
     3361 cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc ggcggcgata
     3421 gagataacca caaaaaatcc gctccagtgc caggtatcga gtactttcgc cagcggccaa
     3481 ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc cgcccctgcc
     3541 gcctctttgt gggaacactc tgccgccgcc ataccgatta acatctgtgg gccaaagaca
     3601 aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa tggcatcagc
     3661 cacagggagc cgactgaaag caaaattccg gcggcgaaaa tcaaattcat cggccctcgg
     3721 ttgccgttaa acaatttgtc cgagccccaa cccgctacca gcgcaccgat aaatccaccc
     3781 agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag cgtctcggac
     3841 atatacaaat tgccccagtc gttgatcgcc gcccggacca catagaccag cacatagcaa
     3901 aacgaaagca gccagatata cggattcagc aacacatatt tggtgaggat ctctttacgc
     3961 gtcagccctg ccccttcttg ttgttgagct atctccagcg cgtcgtgtcg ccattcaccg
     4021 accgccggta aacctaacgc ctgcgggcga tcgcgcagcc gccagcagag aaaaattccc
     4081 acgacgatcg ccatacaacc agcaatcatc atcccggcac gccagccgta atgtagtgca
     4141 gccgctgcca tcacaatggg aatgagtgcg ccgccgacgt tatgcgccgt gttccataat
     4201 gcccaccaac cgccgcgctc ggtacgtgaa taccaggccg ttaacagacg cgcacacacc
     4261 ggtgaacccc agccctggaa aaaggcgttc agcacccaga gcacggcaaa cgcccatagc
     4321 gacgtcgaga agccaaacag aatgttgata atgcccgtgg cgataagccc tatccccata
     4381 aaataacggg catttgagcg atcgctgaca atgccggaga caaacttcga cacgccatag
     4441 gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac gccgttagca
     4501 aggatttctg gtacggcggc gttaaaactt ttccgcgtga agtaaaacag cgcataaccc
     4561 agccagatgg tcagcagaat atgccgacgc cagtagcgat agcgggcatc aatttcatat
     4621 ttatcagtca ttaatggcgc atcggcaggc gctttcagaa acggcaacat catcaaacct
     4681 tagacatagc gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca ggaaatgtgt
     4741 aatgtgccac ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa accttgttgc
     4801 ccggaacccg gcggcaaacc gctgccatcg tcttcaataa ccagcatcaa ccgttcatcc
     4861 tgctgccagc cttgcagcgt gaccgcgctg gcatcagcat gtttcacaat gttgttcagc
     4921 ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cacttaatgc tgattcatcg
     4981 attcgccatt cgagatggct gacaataccg cgcccttcca gctccatttc ccgcatcagt
     5041 gagcggatgg cctgctccag ggtgagatca tccaactggc gcggacgtaa ccgacccaac
     5101 aaacggcgca ccgcgtcgta aacgcccagc gatagttgtt cgatgagctg cccgctctgc
     5161 ttcacgctgg cgttatctgc cgccagccgc tgaacaatgc ccgcctgagt acgaatagca
     5221 gtgatggtct gaccgatatc atcatgcagc tcacgcgcca catcacggcg cacgctctct
     5281 tcggtttcca gcaaccgttc agccagatgc tgattgcgcg ccagttcctt ttgcagcgac
     5341 tggttaagtt cacgcaaccg ctggatgcca gcgccaagca acaaccctgt cagactttgc
     5401 accagcagcg agagcaataa atccaccgga tgatcgcgcc aggtttgact ggcgatcagc
     5461 gcgatggcgt tcatcaacgt cgcaatcagc gccccttgcc aaccatagtg ccaggccagc
     5521 gcgataatcg gcagcgccag acagaatggc gtaaagcgcg acagttcgtc cggcaatccc
     5581 aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg tcgcccgcgc
     5641 cagttgattg gctgagaaac cagtgacggg ccgagcggca gccaggtgtt attggcgaga
     5701 tagtgccaga acaccagaca tatcggggcc agcgtcaggc cgccagttaa agtcagcaac
     5761 agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa caacgccgcc
     5821 gccgttaacg ccgccccctg taacagcaag gtgcgccagt cacgctgatg gcgatagcgc
     5881 gagatcagcg ctacgggcag taacgtcagt aaactaccga tcatcaataa cggaaaatgg
     5941 gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgcacc cagcaatacg
     6001 ggccagtatc cgcgcgggca ttgcagcatt agccccagac gcagaccaaa cggaaataac
     6061 agcaccgcca tatcagggcg ctcaaccaga tgcaggctga tactccacag gcaaaaccat
     6121 gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa caacgtcttc
     6181 atcaccagcc atcaaacatg cggcgcgcca gctctacgtc gttactgaca cccagttttt
     6241 ccatcaggtt ggcgcgatgg acgtgcaccg ttttcggcga caagcccagt tcggcagcaa
     6301 tctctttcac cgccattcct tgcgccagtt tttccgccac ctgccgttcg cgtttggtta
     6361 gcgggtcctg acgaccggat gccagtttaa tggcaatatc cggcgtcaga taacagccgc
     6421 ccgtggcaac cgtatgcacc gccgcaatca gttcgtcagg gctacagcgt ttggagagaa
     6481 agccgcgtgc ccccgcgtta agcgcctgct caaccagcgc cggactgtca tgaacagaga
     6541 gcattattgt cgccatacct ttcggcagct ggcttagcag ttccagaccg gagatatcgg
     6601 gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc gccagcgcct
     6661 cgcgccccga accaaactcg gcaactacct gcaaatcagg ttccagcccc agcagctgcg
     6721 caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc atggtcttgt
     6781 cctggcgggt aaaaaaaaac gcgcttacct taacgataag cgcgatgttg ttcaagcctt
     6841 gagcggttac tgaaaaaaca ccgcaatctt gttaaacata gtcggatcgg actgattacg
     6901 ctgcactttc accacatctt ccagcttatc gatttggctt atcatctgct ccagacgctg
     6961 gtcgtcattg accagtagcc agatatggct tttgtcgctg tcctgaatcg gcagacaaag
     7021 aatgccttca acgttaaaag cgcggcgggc aaaaaggcca caaacgtggg tcattacgcc
     7081 cggatggttg cgaacggtga gctccagaat cacgttgtca tgagttgtgt tttgcatggc
     7141 ttattccccc accatttcag tattcgccgc acctggcggc accatcgggt aaactttttc
     7201 ttcggcatca atgcgcacat ggatcagcgc cgggccaggg cgattgatga tttcctgcaa
     7261 tgcagcctgc ggatcggctt cgttattcaa atcacaggtt tcgaggccga atccggcggc
     7321 aatctgcata aagttgattt ttcccggata ggtggcggca aaaacgcctt gctcgtagaa
     7381 cagactctgt tgctgatgca ccagccccag cgcttcgttg ttcatcagaa tgattttgac
     7441 atccagctga ttttcactgg cggtcgccat ctcctgaata ttcatcatca ggctgccgtc
     7501 gccggagaaa cacaacactt tgcgatccgg gttcgccagc gccgcgccaa tcgccgcagg
     7561 caggccaaaa cccatcgtgc ccagcccacc ggaggtcagc cactggcgtg ggcgattgag
     7621 cggataagct tgcgcggtcc acatctgatg ctgacccaca tcggtggtga taatcgcatt
     7681 gtcatcgaca caggcggcaa cggcgttgat caggccgtaa tggcttaatg gatcgcacgc
     7741 tttcgggatt ggacacggga attcgcgctg caaatccgct accaactggt gccactctgc
     7801 acgcggttgc gcttccacct gcgggatcaa ttgcgccagc acgtcatcaa catccgcctg
     7861 aatcgccaca tgcggctgct tgattttacc cagctctgcg cgatcgatat cgacatgaat
     7921 aattttggca ttcggacaga actgctcggt tttgccaatc gcccggtcat caaaacgcgc
     7981 accgagcact atcaacaaat ccgcctcctg caaaatatag ttggtgctgc gcacgccgtg
     8041 catccccagc atacccagcg acaacggatg cgcttttggc aacatgccca gcgccattaa
     8101 agtcatggtg gtaggcagtt gcgctttctc cgccagttca cgcacccgtg cgggcgcatt
     8161 gatcacaccg ccgcccagat aaagcaccgg gcgtttggca gcgttaatca tcgccgctgc
     8221 gtcacgaatg ctttcttcgc taaaggcggg ggcggcggct ttttctgcca tagcgggctg
     8281 tgtttcaatc tcaaaaactg ccgtttgcac atccttagga atgtctatcc acaccgggcc
     8341 tgggcggcct gattgcgcaa tgcggaaggc atcgctcatg acctgcggga gttcttcgat
     8401 atgtctgacc agatagttgt gtttggtgat ggggatagag atgccgtagg tgtccacttc
     8461 ctggaaggcg tcggtgccga tcatcgaggc gggaacctga ccggtgatgc aaatcagcgg
     8521 gatggagtcc agccgcgcat cggcaatggc ggtcaccagg ttagtcgcac ccggtccgct
     8581 acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc atcccctgag cgataaatcc
     8641 cgcgccctgt tcatgacggg ccagaatatg gcggatttgc gtgctttggc ttaaggcatc
     8701 gtaaacaggc aggatagaac cgcccggaat gcccgtcaca atcttaatgc cctgctgttc
     8761 caggaaatga acgataaatt ctgcgccggt aaagcgctta cgcgtcgatg ttgtgcccga
     8821 acttgccatg ctccagtcct tttcttctgg gccgactttc cgggaggtgc ttaaacgaaa
     8881 aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg tgttgttcca gtccctacgg
     8941 cgcattgccg acgaccacca ccacacgcac gacgaccact gcggcggata gcgcagttgg
     9001 tagtagtttt gcgttgaaca tggaagtagt cattggggac cttgttggtt ttgtgtttaa
     9061 caatatttat acaagcacag ctttacaggg gagacaatgg aaaatttttc agcaagggaa
     9121 aattgagggg ctgatcacgt tttgtactga attgcaggta acaaaaaacc ccgccggagc
     9181 gaggtttcat cagccgcctg cggctggtaa ccgcaaagca cactgtatta tgtcaacact
     9241 gaaagtatac gtgttccgcg cagaacgcgc aatttcggca cgaattttga cgtatttagg
     9301 gcatagttga gtatcgatca cagtttgcgt tttgtccaaa tattactgtt tatttataca
     9361 gtaaacttct ataatatcac tgtacgcaat gtgttatgcg ggggccgcat cgttacccgg
     9421 cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc cttaaccccg cgtgagcaca
     9481 ctgtgttatg tcaacaagca caacgtttct ccttaagata ccgcgtgcac aacagctggc
     9541 aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga gacgcgtatg
     9601 agcctggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact gcaactgctt
     9661 gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcaccaaag gggagcgggt
     9721 aaccgctccc cttttatatt tagcgtgcgg gttggagttg ggggcttctt ctaccagcag
     9781 aatgtttgca cctgcgctgc ctgtgcccct caccctagcc ctctccccaa aggggcgagg
     9841 ggaccgtccc gtgcaatgtt tagctcattt tctgaaaggg aacggtggtt tccccctcgc
     9901 ccctttgggg agagggttac agttcgcacg taatccctcc cggcaacatt tattgccgct
     9961 tttgtttaca tattctgccg ctaaacaatt ccccattcct ggcgtatatc tggctaacat
    10021 tcatcaatgt gatagattcc tctcccgcat ttatgggaat gcgtagtgac ttattctaat
    10081 tatttttata aaagcatccg tgataatgaa aaggcaaaga aacgtcaatt tgttattgat
    10141 gttggtatta ctcgtggccg tcggtcagat ggcgcaaacc atttatattc cagctattgc
    10201 cgatatggcg cgcgatctca acgtccgtga aggggcggtg cagagcgtaa tgggcgctta
    10261 tctgctgact tacggtgtct cacagctgtt ttatggcccg atttccgacc gcgtgggccg
    10321 ccgaccggtg atcctcgtcg gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac
    10381 gacctccagt ttgacggtgt tgattgccgc cagcgcgatg caggggatgg gcaccggcgt
    10441 tggcggcgta atggcgcgta ctttgccgcg agatttatat gaacggacac agttgcgcca
    10501 tgctaacagc ctgttaaaca tggggattct cgtcagtccg ttgctcgcac cgctaatcgg
    10561 cggtctgctg gatacgatgt ggaactggcg cgcctgttat ctctttttgt tggttctttg
    10621 tgctggtgtg accttcagta tggcccgctg gatgccggaa acgcgtccgg tcgatgcacc
    10681 gcgcacgcgc ctgcttacca gttataaaac gcttttcggt aacagcggtt ttaactgtta
    10741 tttgctgatg ctgattggcg gtctggccgg gattgccgcc tttgaagcct gctccggcgt
    10801 gctgatgggc gcggtgttag ggctgagcag tatgacggtc agtattttgt ttattctgcc
    10861 gattccggca gcgttttttg gcgcatggtt tgccggacgt cccaataaac gcttctccac
    10921 gttaatgtgg cagtcggtta tctgctgcct gctggctggc ttgctgatgt ggatccccga
    10981 ctggtttggc gtgatgaatg tctggacgct gctcgttccc gccgcgctgt tctttttcgg
    11041 tgccgggatg ctgtttccgc tggcgaccag cggcgcgatg gagccgttcc ccttcctggc
    11101 gggcacggct ggcgcgctgg tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc
    11161 gctctctgcg atgttgccgc aaaccggtca gggcagcctg gggttgttga tgaccttaat
    11221 gggattgttg atcgtgctgt gctggctgcc gctggcgacg cggatgtcgc atcaggggca
    11281 gcccgtttaa gcgcacgtca ccgcagcatc gtcatcagct ccatgggaga acgatgctgc
    11341 tttatcagat cacgcatcac ccgcatatgc ggtgcggagt aagaataaaa cgcctgatag
    11401 cccgcacaaa gcacgctgtc tggcgtctcc tggcgagcgt gcgggcacag gcgatgccag
    11461 tcgcacgcct ggcagtcacg gcgcggttgc gggttttcgc tgccacacca tttttgtaag
    11521 gtggcgtcaa aaagctcaat cgagatacga ctgatatctg cccgcaccca acactcgaac
    11581 agggcaatta aaaatttccc ccacagctct gctgtcaccg aagcggccgt cagatcgccc
    11641 cggtcatcaa attcaagata gggaatgagc tgtacggagc ggatgccccg ctgtttgaaa
    11701 ttgtgataaa aatcggcagg ttgcagagca tccttgtact ctacaaccag tttatgcctg
    11761 tcctcttcag catcaatgac ctgacttcct gtcatgcgac tctctcaata aatacgataa
    11821 gttgatgtat atgcgttaca gcaaacagta ttgcgagaga taacaccgcg agggagatca
    11881 aaaatttgtc acggactacg tgaaattggg aaaaatcgct attcgtgaca tccattaaat
    11941 tgcgattacg ggtgtagtgc cagaggatca gcgccacgat ggcgagaatg ccaatcgaaa
    12001 tccagaataa catgcccgcc tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc
    12061 cgtagcccag catggtacga aaccacgcca gtgatgtgcg ctccggctgc agtcctgggt
    12121 cggcaatgcg tctggctttg cggctatccg gcatacaaca ccagtcccat aacgatcacc
    12181 gccacgacca tcaatattaa gctaatgatt aataagctgt tggtgtaggg caggtcctct
    12241 ttcagacgca tcgctttttc attgcgtaac cagcgcaggt agccatacat cgctaacccg
    12301 ccggaaaaca gacacaacaa caacgccagc agttcacgaa tgacgggcgt ggcgaaatct
    12361 ggcgcaagtt gatcaagccc aacccccgcc gccagaaatc ctagtgcggt acggatccac
    12421 gccagaaaag tacgctcatt agccagcgag aagcggtaat cgggcgcttc tccaaggcgg
    12481 gaaattttca ttgttgctcc tttgcaattt cactaacgct ctacctttag cggttttttt
    12541 ttaagaattt cgtccgcccg tttcctgata tgctttcgcc atccgctaca aggaggtcag
    12601 atgggtatca tagctcaaaa taaaatcagt tcattgggca tgctgttcgg tgccatcgct
    12661 ttaatgatgg ggattatcca cttttcattc ggcccttttt ccgcaccacc gccaacgttt
    12721 gaaagcatcg tggcggacaa aacggctgaa attaaaagag gtcttctcgc cggaataaaa
    12781 ggcgaaaaaa ttaccaccgt ggagaaaaaa gaggacgtag atgtcgataa aatcctcgat
    12841 caaagcggta tcgcactggc cattgctgcg ctactttgcg catttattgg cggtatgcgt
    12901 aaggaaaatc gctggggcat tcgcggcgcg ctggtttttg gcggcgggac gctggctttc
    12961 cataccttgc tttttggtat cggcatcgtg tgcagcattt tgctgatatt cctgattttt
    13021 tccttcctga ccggcggttc actggtttaa gcagcctgaa taatgcccgc cgggtgttca
    13081 tcagcgggca ttatcgtcag aacttctgtt ttttctcttc cactttcact ccttgcgtcg
    13141 gtaggccggt gtcatagtca cgtaccaccg gcgaatagcc atcttgtggg cgtggacgaa
    13201 atgcccccat ccagcgcggc agtgcatctt tacgccacgg acgcaggctc cattggtaac
    13261 tgcggaacgg atcgcgaatt ttgtccatgt aatccaataa ggcgtcatgc attttgctgc
    13321 gaacgtctgc aaaacggata tcatcgatca ggttatgcat ttcatttggg tcattacggc
    13381 gatcgtaaag ttcatcactg gtgaagaggt tgagtaccag tttaaagtca tccgtcaccc
    13441 agcaacgcac cggaataaaa ccgccaaagc tgtcatgctc aatctcgtag cggttaaatt
    13501 ccaccattac accgcgtggc tctttcacgg caaggatatt ttcccccggc agaatctctg
    13561 gtttttcaat atctgccagc gccatcattg tcggcagtaa atcgatatga ctgactggcg
    13621 tatcgacctg tcgccgctcc ccttgcggcg aacggatgat cagcggaata cgggtgatgt
    13681 cgtcatacat cgccgctcct ttactgatca gcttatgtgc gcccatcatt tcgccgtgat
    13741 cggaagtata aatgacccac gtattttcac gttgctctgg cgttaaggca ttgatgaccc
    13801 gtccgatttg gtcatcaaca aagtcattac aggcaaaata gagcggatgg tgataaagcc
    13861 cgtcatcacc gactggcgat ggcatcgcct gcgcccataa gcggtgatgt tccggtttgt
    13921 tcgccaggtc atcctgagct ttctcgccca gatcgtagta aaaatcagcg tatttctcta
    13981 aatactccac cggacaggtg aacgggtgat gcggctcatc atacgaaacc accatcagga
    14041 agggttcctc ggcgcgcgcg ggctgttgca gaaaatctac cgcccgattg ctgatgcggt
    14101 gcgcccaggt gaaggtttcg tcgatatggt tcgcctgtaa atcctcaacg ctgtttaggc
    14161 cattgcgcca caggctgatc tctttttccg tcagttcgct aaggtagttc gccccatcga
    14221 accagtaatc agcgtcccac tccggcggac actcgccagt gccgaaatag tcatgaccgt
    14281 cgagatgcca tttgccgatg taacaggtgt gatagccggc atccttaaag tagcgcccca
    14341 tagtggagat gtttttgcct ggcgcgacgt tgttggtcca cgggccggac tggttagcgt
    14401 agataccggt aaatagtccg gcgcgtgcag gcgtacaaac cggtgaacag gtgtaggcgg
    14461 aattaaagcg aataccttcc gccgccagac tatcaatatt ttgcgtattc agcggtttac
    14521 cgctatagca accgaccata ttggtggcct gggtatcggt catgatgaac agaaaattgg
    14581 ggcgtttcat aggctatcct tcgcgtcggg agtaaaggtt acggctgggt cgtgacgatg
    14641 ccgccagctg tcaaaaatca ggtagacaat cactacggcg gcaatgaaat aactgatcat
    14701 cgctaaccag cgcgtaccgt agccgccgaa ttcagccagc ccggcataga cgccaatcat
    14761 cgcgaacagg atgccaattg acgcgatctt gacgtttctc cacggtttca tgtctaccgc
    14821 aaacgcatct ttgaaggtga acggcgtggc gcgcggtttg ataaaaccga tcaccagcat
    14881 cacgaccacg ttgatacaga acgtacaggc cagcacatag aggaaatgga agtcgaactt
    14941 caccagatag ttgatggtga tgtagctgat tatgccaatc cccatcgcca cttttgccgc
    15001 cagcgccggg atgcgcggga agaaaaagcc cataatgatg atggtaacca gcggcacgtt
    15061 gtaaatgccg ttgagctgtt tcatccagct atacagcccc tgcggcgcgt tggcgatcca
    15121 cggcgcgacc agcaccgaaa cgatagcgat aaagaaacca aatttgcgcc cgacggtgac
    15181 cagctgctgc ggctcggcat tctggttaat gatgcgacgg taaataccca tactgaataa
    15241 cgtactggcg ctattcagaa agccgttgaa ggtactgatc accgcaccaa ataacaccgc
    15301 gccgaagaaa cccaccattg gcactggcag aacgttattg accagcgtcg ggtaggccat
    15361 gtcggctttc ggtaaatcct gatacagatg aaacgcaatc aaccctggca gcaccagtac
    15421 cagcgggtcg agcattttca gcaccgccgt taacagcgcc cccttttgcc cttccgccag
    15481 gctttttgac gccagcgtgc gttgcacgat gccctgattt gtacaccagt aaaaggtgtt
    15541 caccagaatc aaaccggtaa atgccgcgcc aatcggcaag ggatcggtcg ggccaccgat
    15601 tgagtttaat ttctcggcgt gaacggtggt gagttgctca atgccctgca taaagctgcc
    15661 cttgcccatc gcgatcaggc caaataccgg caccatcaac ccgccgataa ccagcccaat
    15721 accgttgatg gagtccgcca ctgccattgc gcgcagtccg ccgatcaccg catacaaaat
    15781 tcccgccaga ccaagcaaaa ttaccagtag ccagatagcc gcaccgtgag aaatctgtag
    15841 cgattccccg acgtgaaaca ggctgttgag cgccaacgcg ccggagtaga gaacaatcgg
    15901 cagaaagcag acgccggtgg caatgaggaa gcagaagtcg atgataatac gcgtcgtttt
    15961 atcataacgt tcctccagaa aatcggggat ggtggcaatg ccgcgcttca gatagcgcgg
    16021 taaaaagatt agcgcgagga agatcagcgt caccgctgaa gtcacttccc agcccatcac
    16081 cgacatgccg cttttgtagg cctgcccgga aaggccgacc agttgttccg tggaaaggtt
    16141 ggttagcatt aacgaagcgg caataaccgg cgcttttagt gaacgtccgg caagaaaata
    16201 gccttgttgc gatccggtat ctgttttgcg gaccttccac caggtgatca ccgccaccag
    16261 cagcgtaaaa ccgacaaaac tcaagatttg taacgaattc atctcatagc ccttatttat
    16321 gtttatgtcc cggacgcatg aatataggga gaggtaaaat gcgtcgggtc aggcagtcag
    16381 attcttaaaa tacaaacgtc gtatccctga acggattgtt ttctgttaag ttcaggttgt
    16441 gagcataatt cgccggaggg atattaaaat gaatggaaaa ttgcaaagtt cagatgtaaa
    16501 aaacgaaact ccgtacaata ttcccttatt gattaacgaa aatgtgatct ccagcggaat
    16561 ttctctgatc tcgctctggc atacctacgc cgacgagcat taccgggtga tctggccgcg
    16621 ggacaagaag aaaccgctta tcgccaactc atgggttgcg gtgtataccg tacaaggatg
    16681 tgggaaaatt cttttaaaga atggcgaaca aataacactg catggcaact gtattatatt
    16741 cttaaagcca atggatattc actcttatca ctgtgaaggt ttagtctggg aacagtactg
    16801 gatggaattt acccccacca gtatgatgga tattcccgtt ggtcagcaaa gcgttattta
    16861 taatggcgaa atttataatc aggaactcac cgaagttgct gagttaataa cttcaccaga
    16921 agcaataaaa aataatctgg cagtcgcttt tctgacgaaa attatttatc agtggatttg
    16981 tcttatgtac gcagatggta aaaaagatcc acaacggcgg caaattgaaa aattaattgc
    17041 cactttacat gccagtctgc aacaacgctg gagcgtagct gatatggctg ccacgatccc
    17101 ctgtagcgaa gcctggttgc gtcgtctgtt tttacgctat accggcaaga cgccgaaaga
    17161 atattacctc gatgcgcgtc tggatctggc gctatcgcta ttaaagcaac aaggaaactc
    17221 ggttggcgaa gtcgctgata cgctcaactt cttcgactcc tttcatttca gcaaagcctt
    17281 taaacataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
    17341 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
    17401 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgaca acgtaatcgc
    17461 ctaccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
    17521 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
    17581 aggccgtagt agcgcacgcg catctgtttg cgatcttgca gcccaacaat ttgcgccatc
    17641 cggctttcaa taccgattgg catgtcacag atgttgagga ttttcgcatt cgggcgcaga
    17701 cgacgcgtgg cttctgcgac aattgctgcc gggttggagt agttgagcat ccaggcgttt
    17761 ggtgaatatt tttccatata atccaccagt tccaggacgc cgccaatgga acgcatgccg
    17821 tacgctattc cgccgggtcc gcaagtttcc tgaccaacaa caccgtggcg cagtgggatt
    17881 ttttcatcca gttcgcgcat cgggtatttg ccgacgcgga tgtgcgccat gacaaaatca
    17941 acgtcgctga acgccacttc aggatcggtg gtgtaactaa acgcaatgtc cggcgctttc
    18001 tctttaagga tgactttaca ggcttcggca atcacttcct gccgcgcgcc atcgttatca
    18061 taaaatttca gtgcacgaag cgggaaacgc tcctgattcg ccaggagcat caacacgatg
    18121 cctggcgtaa aggtgcttcc accgcctgcg acaaccactg agaatttggt cattatactg
    18181 cctccgtaat ggcaacattt tctgctgatt gatgagaatt aattaagcta tcgagctgtt
    18241 cacgcagctg ggatacatgc agaccaatga tcacctgaat ggcatcgcca ctacggaaga
    18301 cgccgtgcgc tcccagcttt ttaaagactt cgtcatccag cgtttgtgac atgtcatgca
    18361 gtgcaatgcg tagacgcgtc gcacaattgt taatgctgga gatattgccg accccgccca
    18421 gggcttgcag gataccggca gcctgatcca gctctttttt tggctctgcc gcggtggttt
    18481 ggcctcgcga ggctttgtat tcggcttttg agtagagttt cacttccgca tcttcacgtc
    18541 ccggcgtgca catgttgaac tgcagaatca gtgtgcggaa aaccacgaag tacagcaggg
    18601 taaagcacaa cccaatggcg atttgggtca gcatcatatc cgcgtggttg ctgaacatcg
    18661 ggatccagtt ttgcggtaaa acctggtcaa tcagacctcc gcccatgttg cccaccacac
    18721 caaagagata catcacggtc gacattgagg ccgcaagcac agcgtgtacc gcaaacagca
    18781 acggtgaaat gaacaggaag gtaaattcca gcggttcggt aattcccacc agcatggcgg
    18841 ttaaggttgc gggaatcagc aagcccgcca cttttacccg attttccggt gcggcagtga
    18901 agtacatcgc taaagaaatg cccacggcac caaagatttt tgagttaccg tgcagggcaa
    18961 aaccgccttc cgggaacaac gatttcagcg gctcggcgct caaactgaac tcttgcagat
    19021 gctgcgccca gtacatctga atgccgcctt caacagctgc cggaccaaag ataaactgtc
    19081 cgtagatgaa gtggtgtaaa ccggttggga tcagaatacg ttcgaggaag gtgtaaaccc
    19141 agaccccaag tgcacccgcc gaacgcagga acgcttgcag agattcaatc cccatttgta
    19201 cttttggcca gccgagcaac gtcagccagg cacaggggat catcaccagg aaggcgataa
    19261 tcaccacata agacgtcccc tggaaaatgc cgagaaaaac cggcagtttt ttatcgaaca
    19321 gacggttatg cagcgccgtc acaatgccgg aaatgataat tgcgccgata atgctggtat
    19381 cgagggtttt aatcccggcc atcattgtca gaccgctacc tgccaccgcg tcctgagtga
    19441 aatcgacgcc gaagtagctt ccccaggtca ttcccatcgc gttgatgaaa tagttccagg
    19501 tcaggaaact caccatcacc gccagacaag cacgcccctg cgcttgctta gcaaggccaa
    19561 tgggtaaacc gacagcaaaa atcagcggca tattacggaa taccgtccaa ccgccctctt
    19621 caataatgtg tacgatttgc gcgaataaac tgttcggatc ggtcagtgat tccccgacaa
    19681 acatcgggtt ttgcagcaag atggcaagac ccaccacaat cccggcgaag ggaaacagca
    19741 gcactggcgt gaacatcgcg ccgccaaagc gttgaatttg actgagcatt tccacatcct
    19801 cttttctcaa ttctgtaagt aggaagcctg attggttatg cggcaggcct ggagcgagga
    19861 taggaagatg cgatcggcag aacatgacga cccaatggga ttcgtgatcg ctttcatgct
    19921 tttcgttggg cggtagagat ctacgttttc gcgaaaaagt agacatctgt gagcacaggt
    19981 acacagcatc gaacacatct ttaaaaaaaa gatgtttttt caatcgatta agcagaactt
    20041 gtgggcgcat tacccgtgct tgcaggcaaa aaagagatct agagatgatc tacaaaagca
    20101 ttgcggagcg gttaagaatt cgacttaatt ccgcagattt cacgctaaat agccttcttc
    20161 ccggtgaaaa aaagttggcg gaagaatttg cggtatcgcg gatgaccatc cgtaaagcca
    20221 ttgacctgct ggtagcgtgg ggattggtgg tccgccgcca cggcagcggc acttacctgg
    20281 tgcgcaaaga tgtactgcat caaaccgcca gcctgaccgg gctggtggag gtgttaaaac
    20341 ggcagggaaa aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg
    20401 ccattgccag ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc
    20461 gttttgtgga agggaaaccg ctgatgctgg aagacagcta tatgccggta aaactgttcc
    20521 gtaatctttc gctgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg
    20581 ggattttgat tggcggtaat tatgaaagcc tgacgccagt gctcgccgat agactgctgg
    20641 cgcggcaaat gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata
    20701 gcgagagcgg ggagtttttg aattattcag tgatgttcag aaatgccagc gaataccagg
    20761 tggagtacca tttacggcga ctccacccgg aaaagagtta accgatactc cagaagagca
    20821 ccgccagtaa ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt
    20881 aagagagcgc cgccgcaccg ctggttggat gaagattatt agcaaacgcc agcgccggag
    20941 gatcggtcat ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca
    21001 tccgcgccag aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa
    21061 tccagcttag cccttcgcca ttgaccaggg tattcacaaa atctccacca gatttcagac
    21121 caacgaccga gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg
    21181 gcataaacca gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca
    21241 gcggaccgcc cgccagcccc agtttcaacg cggccgggaa cccaggcaca aagacgggaa
    21301 tagaacctaa caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt
    21361 gcagtttttg ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc
    21421 ccaccagatt gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga
    21481 ccccggcacg gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc
    21541 gaatacgttt tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag
    21601 tgcctttcgt ggacagcgaa gtatcgacct cctgaccaat caccagttgc gcattatgta
    21661 aatccgctgg cagccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg
    21721 aaggaacttt tagggtttct tcgcgtttca gacgcgagca gataattttg tcgccgttga
    21781 gaatcggtac atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga
    21841 tagtcttgat cagcgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct
    21901 cgacattgac gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc
    21961 cgaatggata cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac
    22021 ccaagtcgcg caaaatctgc tgccctgccc ccagcgctgg cgtattggta accgcaccgg
    22081 agaaaatccc cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa
    22141 ccagaccacc gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc
    22201 gcaatgaggc aaagaagccc ggccctacct gaatcccgat agtataaacg aacaggatca
    22261 ggccaaattc ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa
    22321 caaaatggcc gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc
    22381 gaaatttgac attgccgata aacaaaccga cgactgccac caaagccaga atactgaccg
    22441 ttaatgctat atcactcatc gtccatttct cttgagataa catttttaat tgctacggat
    22501 tatggctgaa tcggtgaagc agatttgcct tatagcgcac attatcacgg gaaagaacgt
    22561 gccgaaatat cttaaacagt cgaaatttaa cgcattgttg agtaagtaag acaaaaaaag
    22621 ccccaccaga atggcggggc aaagagaata gctagttagc tatttaacgc gggacgttcg
    22681 ctgatagcga tacgctgcgc tgcgatgggt tcaggctcat tacgaattaa atcaatatgc
    22741 agtaaaccgt ttacgaaggt tgcgccagag acttccatat tttcagccag cgtaaagctc
    22801 aggctaaatg gctgattcat aagcccttga tgcagccatt ttttctcttc ttttggctgc
    22861 tccggcgtgc cttttacgct caggcgcgta ccttccagtt gaatctctaa atcttcctga
    22921 cggaaacctg ccagcgcaag ggtaatgcgg tagtggttat cgtcgctttt ctcaatgttg
    22981 tacggcggga agctctggct ttcaccggcg ttttgcagtg cgttggccag tttgtcaaaa
    23041 ccgatccatt gacgcatcag tggggataaa tcgaagttac gcatagtcat ttctccttct
    23101 aagaagcgag taagtacctg caaatccgaa gattcgcata tgctccctga cggcgagcat
    23161 ggagatgtca ggccgcgcca ggcggcctta gggaattagt tgatttcgat acggcgcggt
    23221 tttttcgctt ccggaatcac gcgttcgaga tcgatataca gcaaaccatt taccaggtta
    23281 gcaccacgaa catgaatgtt ctcagctaac tggaatttgc gttcaaagtt gcgttcagcg
    23341 atgccctggt acagataggt gcgctctttt tgttcgtcgg cgtgagcacc tttcaccacc
    23401 agcagattat cctgggcggt aatttccagt tcgctctcag caaaaccagc cacagcgata
    23461 gcaatgcggt aatggttttc gtctaccagt tcaacgttat acggagggta gccgccatta
    23521 ctctggctct ggttgttttc taagtggtta aacaaacggt caaatccaat agcagaacgg
    23581 taaagcgggg ataaatcaaa gttacgcata atcaatagct cctgaaatca gcgagaatgt
    23641 aagaccttcc acaatggaca ggtcaggtag ccagaacacc catcaggcgc gtcctcatcg
    23701 gctacgatgt aaaaatgggt ctggaaatga acttttcaag ccttatcagc ggactttttt
    23761 ttgcagttta tggtctattg catagactga gggggcagca cgaaacgtta aagtgcgata
    23821 gccgccacag tgcgacgtaa tgatgatgtt attttcagca agggatcgct ataactcatc
    23881 aggccaacac ccacgatcaa cagatgaatt tattatgata agaaatgtgt tgttagcgtt
    23941 catgatatgc agcggaatga cattactcgg aggatgctcc agcgtgatgt cccataccgg
    24001 agggaaagaa ggaacgtatc ccggcacccg cgccagcgcc accatgattg gcgacgatga
    24061 aaccaactgg ggcactaagt cgctggcgat tctcgatatg ccgtttaccg ctgtgatgga
    24121 cacgctttta ctgccgtggg atgtgttccg caaagacagc tcggtgcgct cacgcgttga
    24181 aaaaagcgaa gccaacgcgc aagccaccaa tgcggttatt ccaccggcca gaatgcctga
    24241 taactgattt agcgatcagt ttcggtgatc cacagctggc caccttccat ccacgccagc
    24301 cattgaccat ccggcgagaa gaccacggcg tccgcagaag gtggattggc gtggttttcg
    24361 gttaaatact caacctcgcc gctttgcgca tgggcacaag caattcgatt atccagcaca
    24421 aagcccaacc attctcctga cggatgccag ttaaatgcag actgaatatc cgttttgtta
    24481 tgggttaact ggcgcggctc gccgccctgt ggcgagataa gccacagttg cacaatgccg
    24541 ttatcatcac gcattaaaaa cgcgatttgc gtaccctgcg gattacagcg cacccagtgg
    24601 cgcgggacgt tgactaaccc cggataagcc cgatggtggg taaaggttaa acgtcgctgc
    24661 acgacgccgc gcggtggcgc gggcagggtg gtttccgttc cacttaacgg cgcatcacct
    24721 gccgctttcc agccagcttc atcttgcggt aactcaacga taaacagctc cggcactttc
    24781 tcgccctttg gcgaaagtgt gtcgccaata aacgccagcg cgtgatttcc tacccatcct
    24841 tcttcataag cacgattgat ttcatcgctg ccaggctgcg gcgtgggcgt ggttttactc
    24901 accagcacgc accagtggct accgctgtat tcacgcggat gctgcttttg tacgttgacc
    24961 gggccaaacg gcgcagcaac accgacgttt cgcaaatcca gcgccggatc gagttcatgc
    25021 attacatggt cgttataggt aaagctcacc ctttcaccgt tcgggctaaa gacatgcaca
    25081 tggctgccgc cgcgcagcgc tcctggcgta tacggcgcgg taatatccat tgcatcgaga
    25141 ttgctcacct tgccgccttc agcaatgact ccgcgtcgat gatggaaatc gtaatgccat
    25201 gtttcatcag gattttccgg gccatggatg aaaacatatt tctctgactt cggatgaacg
    25261 gtcaccacgc cgacgtgtgc gccctgtgac gcgcgataga taacctcgac ctcgccggta
    25321 tggatattca cacgctcaat ggtctcgccg gtaaacgacg ccccagaagg acgcacgtca
    25381 aataccagcc actggctgtc gggcgtccag gtattggtat tggtgagcag gtgattacgg
    25441 ggagcaaagg ttatctgttt catggcgcgg tcctgataca aaacgggtcc cgccatcata
    25501 cttcacaagg acttcacttt cagacaatag tcttttgcca tcgtgatgaa tgtgagtgaa
    25561 aagatggaac atttcgacgt ggcgattatt ggcctcggcc cggcaggatc ggcgttggcg
    25621 cgaaaattag caggcaaaat gcaggtgatc gcgctggata aaaagcacca gtgtggtact
    25681 gaaggtttca gcaaaccttg cggcggtctg ctggcaccgg acgcgcagcg atcttttatt
    25741 cgcgatggac tgacgcttcc tgtcgatgtg atcgccaatc cacagatttt cagcgtcaaa
    25801 actgtcgacg tcgccgcatc gctcacgcgt aactaccagc gaagctatat caatattaat
    25861 cgccatgctt tcgacttgtg gatgaaatcg ctgatcccca ccagcgttga ggtttatcac
    25921 gatagcctgt gccgaaaaat ctggcgtgag gatgataaat ggcatgtcat ttttcgtgca
    25981 gacggctggg agcaacatat taccgcccgc tatctggtcg gtgccgatgg cgcaaactcg
    26041 atggtgcggc gacatctcta cccggatcat caaatccgta aatatgtcgc tatccagcag
    26101 tggttcgtgg agaaacatcc ggtgccgttc tactcctgca tctttgataa ttcgataact
    26161 aactgttatt catggagtat cagcaaagac ggttatttta tctttggcgg tgcttatcca
    26221 atgaaagacg gtcagacgcg tttcacgacg ctgaaagaga aaatgagcgc ctttcagttc
    26281 cagtttggta aggcggtgaa aagcgaaaaa tgcacggtgc tgtttccctc acgctggcag
    26341 gattttgtct gcggtaagga caacgccttt ctgattggcg aagcggcagg atttatcagc
    26401 gccagttcgc tggaggggat tagctatgcg ctggatagcg cagacattct gcgttcggtg
    26461 ttactgaagc agccagagaa gctcaatacc gcttactggc gcgctacccg caaactgcgt
    26521 ttaaaactct tcggcaagat agtaaaaagc cgatgcctga ccgcaccggc tttaagaaag
    26581 tggattatgc gcagtggtat ggcgcatatt ccacagttga aagattagcc aacgcgcttc
    26641 acatcgccca ccagcaggat gtaagagagc gcgccaatca acgcgacggc ggagatataa
    26701 accagtgcag gtgcgaaacc gtaaccctgc gccaggtacc ccaccaccag cggaacggtg
    26761 atgccgccca gaccaccggc gaagttaaac acgccgccgg ttaaaccaat caggcgcatc
    26821 ggtgccagag aagagaccag cgaccaggta atcgaagcaa aaccgttgcc gaagaatgcc
    26881 agcgccatca ggcacataat catcatcgga tcgttggtgt agttagcgcc cataatgcag
    26941 gtggagatca gcaagccgca gataatcggc gttttacgcg caaagcccag tgaaaagccc
    27001 ttacgtacca gcagatccgc gacccagcca gagagcagga cgccgacaaa cgccgcgagg
    27061 aatggcaccg tggtcataaa gcctgctttc agcgccgtga ttcctttttc ctgggttaaa
    27121 tagttcggga accaggttaa gaaaaaccac agtgtagaag ccaccgcaaa ttgcccaaga
    27181 taaacgccga tcagtttacg atggaacacc agtttccagt ctttggctgt taacggctga
    27241 cgcgcctctt tcttcaccgg cgcatcacca tccaccagac cgccgccatc acgaatgtaa
    27301 tccagttcag ctttgctgat acctttggtc aggcgcggcg gctgataaac cttaaaccaa
    27361 atcagcgacc agataatgcc gataccacca gtgacaatga acacccagtg ccagctcaac
    27421 atctcctgaa tccagatcag cagcggagtc aaaaacgcca gaccgacaaa ctgaccagac
    27481 gtataaaaac caacggcgga agcgcgttca tgttccggga accagctggt caccatccgg
    27541 ttattggtcg ggaaagcggg cgcttcgaaa ataccggtta tcgcacgcag accaattaat
    27601 gacattaatc ccgtggcaaa gccctggaat aaagtcgcca ctgaccaacc aaatatcgca
    27661 ataaaataag tcacgcgaga acctacgcga tctaaaaacc aaccgccggg gatctggcat
    27721 agcgtataaa gtcaggcgaa ggccgaaaat acatagccca tttccgcttt cgtaatgccg
    27781 aactcttcct gaatatgggc ggaagccacg gccaggttag cgcggtcaac ataacaaatg
    27841 accaccgtaa taaagatcat caccagcgtc agataacgcc gacgccccgg ctttgctgca
    27901 ttaacgggaa tatccatagc gagctttctc cagattttgg gcatagcgaa gccgctcacc
    27961 atgccctgta atttacagag ggttatattt ttgtattgct gttttagtgc ccgatgaggg
    28021 gcttacgtgg caggaattac cactctgcta cgctgttatc ttcatgacgc cagagcggat
    28081 tacgccagtc cggggcattt ttactgaact caataacttt agcttcgtcg atttccacac
    28141 ctaagcccgg tttcgttaac ggtttaaaga agccgccaac catactgaag tcttctttgt
    28201 ttttcacaaa gtcgagtaac tccgcgcctt tgttgtaatg aatgcccata ctttgttcct
    28261 gaagtaccgc gttataggaa acaaagtcga tatgcaggca agccgccagt gcaatcggtc
    28321 cgagcggaca gtgcggcgca agggtcacat cataggcttc tgccattcca gcaattttgt
    28381 agcattcggt aataccgcct gcatgggaga gatccggttg cagaatcgaa ataccgcctg
    28441 cctccagcac gcgtttaaaa tcgaaacgtg agaacatgcg ctcacccgcc gccagtggaa
    28501 tatgcgtttg tgccgccaat ttcgggtagt attcggcttg ttccgccagc accggctctt
    28561 caataaacag cgggcgatac ggctccagtt ctttaatcag cactttcgcc attggcgcgc
    28621 tgacgcgacc gtggaaatca agaccaaact caatctgatt gccaaaagct tcacgaattt
    28681 gtgccacggt gttgactgcc gcatctaccg cgcgggagtt atcaattagc cccagttctt
    28741 cacaaccgtt cagtttgaag gtatcgaagc cgatttcgcg cagggtttta atgccgtcga
    28801 taacatccgc cggacgatcg ccgccgaccc aactgtaggc tttaattttg tcgcgaacca
    28861 ggccgcccat cagttgccag accggcgcat tcagcacttt gcctttgata tcccataacg
    28921 cctggtcgat cccggcaatg gcgctcatca ggattggacc gccacgataa aatccggcgc
    28981 gatacatcac ttgccataag tcattgatgc gcgaaggatc ctgaccaatc aaatagtcac
    29041 ccagctcgtg aactgccgct tccaccgtac gggcgcggcc ttcaatcacg ggctcgcccc
    29101 aaccgaccac gccttcatcg gtttcaattt tcaggaacat ccagcgggga ggtaaacgat
    29161 acgtagtaat tttggtgatt ttcattgcac tgcctctcga tacgccttaa caaatgctgc
    29221 tgcctgctgc gcggtgcgtt ctacggactg cccggcgcga tagagatcgc tgcctaagcc
    29281 cgccccagca caacctgcgt ctatccactg cgccaggttt tctggcgtca cgccgccaac
    29341 ggcaaagact gcgatgtccg atggcaatac cgcttttaac gctttgatgt attgcggacc
    29401 aaaagccgat gacggaaata ttttcagcgc ctgcgcgccc gcttcgagcg cggtaaaggc
    29461 ttcggtcgcc gtcgcgcagc cggggcagac ggtcatgccg tagcccaccg cacggcggat
    29521 tacttcacta tggatattgg gcgtaacgat gagctgacag cccatcctgg cgagcgcatc
    29581 gacctgttca ggtttcagta ccgtacctgc gccaatcaac gccttgtcgc catacgcatc
    29641 aacgatggcg ggaatgcttt gctcccattg tggggaattc agcgggattt caaccgcgtc
    29701 gaacccggcg tcaatcaccg cgccaacatg cgccagcgcc tcgtcgggct taatgccgcg
    29761 caaaatggcg atcagcggaa gtttagtttg ccactgcatg agcgatgctc cttataccag
    29821 cctgaaatgc cgtgtcgccc gccaccgccg tcacgtcgca acccatcgcc tgaaaggctt
    29881 gctggtagcg cgcggtcagc gatgttccgg cgacaagggt gatggcgtgt tgatgggtca
    29941 catagtcgcg catactggca acctctgcgc caatcaacaa accagagaga aattcgctga
    30001 cctgttcgcg gggaagtgtt cccagcacat gcgaggcgcg aacttcaaaa agctgcggca
    30061 atatggcggg cgcattaagg ccgcgctcaa ggccagccgc gaaggcatcg gcagagtttt
    30121 cctgcggcgg caaacctgcg ccaatcaatg agtgatttaa cagtaaatga tgtaattcaa
    30181 cggtcatcac ggtgcgaaaa tcgttgattt gctggctatc ggcctgcacc catttgcaat
    30241 gcgtaccggg cataacataa agagaggaag gagccagagc tcgcgcgccg atcaattgtg
    30301 tttcttcgcc gcgcatcaca ttgtggttat cgtcatgaga gacgcataat ccgggaataa
    30361 tccagatatt gtcgccaaca gacgttaatt gttcgccaat agacgaaaaa cgggcaggaa
    30421 cagataaata cggagcaact ttccagccga cattgctgcc gaccattcct gccatcacca
    30481 ctggcgtatt ttcttcacgc cagtcggtcg tgacttctgc taacaccgca gccggagatt
    30541 ttccgttcag gcgcgtgacg cctgcttctg attgcctgct ctccaggcag tggtcgccct
    30601 gataaagcca ggcgcgcaga ttggtcgatc cccagtcaat tgcgatgtag cgagctgtca
    30661 tgtgatttcc tttaaccttc gtgtcgagct ggcgatcatg gtaagcgccg cctgctctgc
    30721 cgcatcgccg tcctgatgcc gtattgcatc gaacagcgcc ttatgttcct ggagcgtttg
    30781 cggcatgttg gcctcatcgc ccatccaggt tcgttcaaaa accgcccgct gcaacgaact
    30841 gatcgcaatg ctaagttgct gtaacaccgg gttatgtacc gactgcagca ccgcctcgtg
    30901 gtagcgaata tccgcttcgt taaacgcttc gcggtcctga ttgttggcaa tcatctcgtt
    30961 cagcgccgat tcaatctgcg ccagatcgct ggaagtcgcg cgctctgctg cccaacgggc
    31021 aatcgccggt tccaccagat ttcgcacttc gctcatggca ctgataagcc gtgggtcgta
    31081 gtcgttttcc agcacccatt gcagtacgtc agtgtcgagg taattccact ggttacgcgg
    31141 tgccacaaac gccccgcgat aacgtttcat ttcaatcagc cgcttcgcca tcagcgaacg
    31201 gaacacctca cggatgatgt tgcgcgaggt tgcaaactct tcacagagct ccgcctcagc
    31261 cggaagtggc gagccaggta cgtatttgcc gtgaacgatc tgtttaccca gcgtaatgac
    31321 aatgcgatcg gttttattga gagtcatgga gagtccttgt gctctgtatg ttcttctcta
    31381 ctttaccccg atcgatgcat aacgcggcaa ctttgtagta ccagcgtgat gacgttcgcg
    31441 tttgccgtgt gcgtaatgta gtacaaactt atattgttgt actacaattt agatcacaaa
    31501 aagaacaatg cataaaaaaa tgacatgcgt cgggcagaaa tctgaaaagg gatatcaggc
    31561 gctaaacagg agggaaagaa gagtatgctt tcaacggctt agctactcgt ttaaaggatt
    31621 aatcatgaag ttgaatttta agggattttt taaggctgcc ggtttattcc cactggcgct
    31681 gatgctttca ggctgtatct cgtatgctct ggtttcccat accgcaaagg gtagttcagg
    31741 aaagtatcaa tcgcagtcag acaccatcac tgggctatcg caggcaaaag atagtaatgg
    31801 aacaaaaggc tatgtttttg taggggaatc gctggattac cttatcactg atggtgccga
    31861 tgacatcgtt aagatgctca atgatccagc acttaaccgg cacaatattc aggttgccga
    31921 tgacgcaaga tttgttttaa atgcggggaa aaagaaattt accggcacaa tatcgcttta
    31981 ctactactgg aataacgaag aagaaaaggc actggcaacg cattatggtt ttgcctgtgg
    32041 tgttcaacac tgtaccaggt cactggaaaa cctaaaaggc acaatccatg agaaaaataa
    32101 aaacatggat tactcaaagg tgatggcgtt ctatcatccg tttaaagtgc gattttatga
    32161 atactattca cccagaggca ttccggatgg tgtttccgca gcattactgc cagtgactgt
    32221 tacgctggac atcattactg caccgctgca atttctggtt gtatatgcag taaaccaata
    32281 atcagtaagc gggcaaacgc gtttatgctg tttgcccgtc cactgattaa ttcagcacat
    32341 acttctcaat agcaaacgcc acgccatctt caaggttgga tttggtgaca aagttcgcca
    32401 cttctttcac cgaaggaata gcgttatcca tcgccacacc gacgcctgca tattcaatca
    32461 ttgcgatatc gttttcctga tcgccaatcg ccatgatttc ttccggttta atacctaaca
    32521 cgtcggccag tgatttcacc cccgtacctt tgttaacgcg tttatcgagg atttcgagga
    32581 agtacggcgc acttttcagc acggtatatt tctctttcac ttcctgcgga atacgcgcga
    32641 tagcctggtc gaggatggcg ggttcatcaa tcatcatcac tttcaggaac tgggtattgg
    32701 ggtccatttt ctccgcttcg cagaacacca gcggaatggt ggcaacgaag gattcatgca
    32761 ccgtgtagta gctgatatca cggttggcgg tgtacagcgt ggtgcggtcc agggcgtgga
    32821 aatgagaacc gacttcgcga gagagttttt ccaggaaacg atagtcgtca tagctgagag
    32881 cagtttgcgc cacggtgcta ccatcagcgg ccttctgtac cagcgcgccg ttataagtaa
    32941 tgcagtagtc gcccggctgt tccatatgca gctctttcag gtagttgtgc acacctgcat
    33001 acgggcgacc cgtcgttagc acgacattca cgccacgggc gcgagctgcg gcaatcgcat
    33061 ttttaacggc gggtgaaatg gtgtgatcgg gcagcagaag ggtgccatcc atatcgatag
    33121 caatgagttt gatagccatg agttccccag gtagattagt tcctgaccca tgctaacgcg
    33181 attccgctca aaaatcagta caacacccga gggaaaaggg ggatgcaacg cgggttgcat
    33241 gctccccatt tgcttagcgg aagagtttcc ctttcagcag ttccatgcct gcggaaagca
    33301 gatcgttatt ggcttgtgga gacacttcac cttgcggtga gagcgcatca ataatcttcg
    33361 gcaattgttc tgccagtaaa ctggaagctg tactggtatc cacgccaagt ttttgtccaa
    33421 gatcggacac cgcatttgtg ccgagcgccg attccagttg ctcgccacta accgattgat
    33481 tgccctgttg attactcagc caggttgaga gaatggcccc taagccgcca ctttgcagtt
    33541 tttccagcag cacctgaatg ccgccctgct cctcaaccca acttaaaata gcctgatatt
    33601 tccccgcatc gcctttcaga aaggcaccga caacttcatc aaaaagcccc atgataatca
    33661 cctgtaaagc gttacgtgtt gacccaaaaa gtatagattt gcgggatgat aattgcggat
    33721 tgcagaaata aaaagggcgg agatgatctc cgcccttttc ttatagcttc ttgccggatg
    33781 cggcgtgaac gccttatccg gcctacaaaa tcatgaaaat tcaatacatt gcaagatttt
    33841 cgtaggcctg ataagcgtag cgcatcaggc acgctcgcat ggttagcgcc attaaatatc
    33901 gatattcgcc gctttcaggg cgttctcttc aataaacgca cggcgcggtt caacggcgtc
    33961 gcccatcagc gtggtgaaca actggtcagc ggcaatcgca tccttaacgg taacgcgcag
    34021 catacgacgg ctttccgggt ccatggtggt ttcccacagc tgttccgggt tcatctcgcc
    34081 cagaccttta taacgctgga tggagaggcc gcgacgggac tctttcacca gccagtccag
    34141 cgcctgctcg aagctggcta ccggctgacg acgctcgcca cgttcgataa acgcatcttc
    34201 ttccagcaag ccacgcagtt tctcacccag cgtgcagata cgacgatatt cgccaccggt
    34261 gataaactcg tgatccagcg gatagtcagt atccacaccg tgggtacgca cgcgaacaat
    34321 cggctcgaac aggttttgtt cggcattggt gtggacatca aacttccact ggctgccgtg
    34381 ctgttctttg tcgttcagtt cgctgaccag cgcgttcacc cagcgggtaa cggtctgctc
    34441 atcagagagg tcggcttccg tcagcgtcgg ctgatagata agctctttca gcattgcttt
    34501 cggataacga cgctccatgc ggttgatcat tttctgcgtc gcgttgtact cagacaccaa
    34561 tttctctaac gcttcgccag ccaatgccgg tgcgctggcg ttggtgtgca gcgttgcgcc
    34621 atccagcgcg atagagatct ggtactgatc catcgcttcg tcgtctttaa tgtactgttc
    34681 ctgcttgcct ttcttcactt tgtacagcgg cggctgagcg atgtagacgt gaccgcgttc
    34741 aacgatttcc ggcatctgac gatagaagaa ggtcaacagc agcgtacgaa tgtgcgagcc
    34801 gtcgacgtcc gcatcggtca tgatgatgat gctgtgataa cgcagtttgt ccgggttgta
    34861 ctcgtcacga ccgataccac agccaagcgc ggtgataagc gtcgccactt cctgagaaga
    34921 gagcatctta tcgaagcgcg ctttctcgac gttgaggatt ttacccttca gcggcagaat
    34981 cgcctggttc ttgcggttac gcccctgctt cgcagagccg cccgcggagt ccccttccac
    35041 caggtacagt tcggaaagcg ccggatcgcg ttcctggcag tctgccagtt tgcccggcag
    35101 gcccgctaag tcgagcgcac ctttacggcg ggtcatttca cgcgcgcgac gcgccgcttc
    35161 acgggcacgg gcagcatcga taattttgcc aaccacgatt ttcgcgtcgg ttgggttttc
    35221 cagcaggtat tctgccagca gttcgttcat ctgctgttca accgctgatt tcacctcaga
    35281 agaaaccagt ttgtctttgg tctgggagga gaatttcggg tccggcactt tcacggaaac
    35341 gaccgcaatc aggccttcac gcgcatcgtc accggtggcg ctaactttgg cttttttgct
    35401 gtagccttct ttgtccatgt aggcgttcag ggtacgggtc atcgccgcac ggaagcctgc
    35461 caggtgagta ccgccgtcac gctgcggaat gttgttggta aagcagtaga tgttttcctg
    35521 gaagccatcg ttccactgca acgccacttc gacgccaata ccgtcttttt cagtggagaa
    35581 gtagaagata ttcgggtgga tcggcgtttt gttcttgttc agatattcaa cgaacgcctt
    35641 gatgccgcct tcatagtgga agtggtcttc tttgccgtcg cgcttgtcgc gcagacgaat
    35701 ggaaacgccg gagttgagga acgacaactc acgcagacgt ttcgccagaa tttcatattc
    35761 gaactcggtc acattggtga aagtttcgag gctaggccag aaacgcacca tggtgccggt
    35821 tttttcagtc tcgccggtaa ccgccagcgg ggcctgcggt acaccgtgtt cgtagatctg
    35881 acggtgaatt ttaccctcgc gctggataac cagctccagt ttttgcgaca gggcgtttac
    35941 taccgaaaca ccaacgccgt gcagaccgcc ggacacttta taggagttat cgtcaaattt
    36001 accgcctgcg tgcagaacgg tcatgatcac ttccgccgcc gatacgccct cttccgggtg
    36061 aataccggtc ggaatgccgc gcccgtcatc ctgtacagag acagagttat cggcgtgaat
    36121 ggtgacgata atttctttac agtgacccgc gagcgcttcg tcgatagcgt tatctaccac
    36181 ctcgaatacc atgtggtgca gaccggtgcc gtcatccgtg tcgccgatat acatacccgg
    36241 gcgcttacgc accgcatcca gccctttcag gactttgata ctggaggagt cataagaatt
    36301 cgacatcaac gtttctcgct catttatact tgggttaatc cgttatttta cccttttcca
    36361 cggtaaacat cttcgaattt tcgtccgaca tgtctataac gtgttcagca ctgatcgcgc
    36421 tgacaaagac ctgtgattgc gtcgctttta agcggctggc aagcagcccg cgacgctcat
    36481 catcaagctc agaggcaaaa tcatctatca ggtagagaca ccgccgcccg ctttcacggg
    36541 tgaggaactc tccttgcgcc agacgtaagg cgcacatcaa cagcttaagc tgcccacgcg
    36601 ataaggtatc ttccaccggc gcaccgtcgg cgcgaatgcg taagtccgct ttatgcgggc
    36661 catgcgcggt gtaggttagc tggcgatcgc gttcaaaatt acgttccagc acctcagcat
    36721 attctgtctc tttctcccag ccgcgctgga aagagaaagt cagagaaaac tcagggagaa
    36781 attgcttaca agtatcggcc atatcagccg cgataccggc gctatactcc gcgcgccagg
    36841 tgctgatttg ctccgccagc gggatcagtt ctttatccca cgggcgtagc tgttcgtaac
    36901 gtgtcacctg gcgcagcgcc gcattgcgct gcttgagcaa tcgcttgaga ttgctccagg
    36961 cggtgaaaaa tccgggttcg ttgtgaaagc atccccagtc gaggaatgct cttctgtatt
    37021 tggggccgcc gttgagtaaa gtaaaccctt ctggcgttat caactgcatt ggcatcaggt
    37081 gcgccagttc cgcgacctta tgcccgtctg taccgtcgat gcggactttg ctgtcgccct
    37141 gtttgtcttt ggttaagcca atcgctgtct cgcgctcttc gccctgtaat cgcccgtgga
    37201 gaacaaacgc ctcctgctca tggcgaatga cgcgaccaat ctgcaaactg cgaaacgccc
    37261 gaccatggcc gagcgtatag atggcttcca gcacgctggt tttgccactg ccgttggcac
    37321 ctaccagaaa gttaaagccg ggagataaag cgagatccgc ggtttcaatg ttgcggaaat
    37381 cgcggatcaa caagcgggtg agggacatta cagtctcatt ggcatgacaa cataggcagc
    37441 cgactgtgat gcggcatctt caatctgcac gctggaaacc gaatcggtca gcatcatgcg
    37501 gacgttttca catttcagcg cgttcagaac atccagcacg tagctgacgt tgaagccgat
    37561 ttccatctcc gcaccactat aggtaacgtc gaggatctct tccgcttctt cctgttccgg
    37621 gttgttggcg gtgattttca gctggttttc gctgacataa agacgtacgc cgcggaattt
    37681 ctcgttagag agaatcgccg cgcgagcaaa cgcctgcttg agcagatcgc agccagcttc
    37741 cagatgtttg tccgggttct tcggcagaac gcggcgataa tccgggaagc gaccatccac
    37801 cagtttggag gtgaagataa agtcgccaac gtgggcgcga atgttgttgc tgccaatctg
    37861 tacgcgcagc ggattgtcgc cgccgtcgag catacgcatc agttcaatca cgcctttacg
    37921 cggtacgatc accgaatggc ttggcaaaga ttgaccaatt ggcattgaac agaccgccag
    37981 acggtggccg tcggttgcca cggtgcgcag ttcttcacct tcggtttcaa acagcatacc
    38041 atttaagtaa tagcgaacgt cctgatgcgc catagaaaac tgggtcgctt caatcagacg
    38101 cttcatcgtt gcctgcggca gggtaaattc gacttcactc tgccagtcat cgaggttcgg
    38161 gaaatccgcc gctggcaggg tagacagcga aaaacggcta cgcccggagc gtaccagcat
    38221 ccgttcacct tccagctgca cggcaatttc cgcgccttca ggcagaccac ggcagatatc
    38281 aaagaatttg cgcgccggaa cggtcgtcgc tcctggctcg tgtggctgaa ccagcgcaac
    38341 acgtgccacc atttccatct cgagatcagt accggtcagc gacaacgtac cgtcagcaac
    38401 ctgtaacagc agattaccga gaatcggtag cgtaggacga ccacctaacg gaccgctcac
    38461 ctgttgtagc ggttttaata aatgctcacg ttctacggta aatttcatag gtttacgatg
    38521 acaatgttct gattaaattt gaaaaatctt ctttgatatc gtggctctct tcacgcaact
    38581 gctcgatctt acggcaggca tgaagcaccg tcgtgtggtc acgaccacca aacgcatcgc
    38641 caatctccgg cagactgtgg ttagtcagct ctttcgccag cgccatcgcc atctggcgcg
    38701 gacgcgccac cgagcgggat cgacgcttgg aaaggagatc cgcaactttg atcttgtagt
    38761 actccgccac cgtcttctga atattgtcga tggtgaccag tttttcctgc aatgccagca
    38821 agtcgcgcag cgcctcacgc acgaagtcga tggtgatcgc ccttccggta aagttggcgt
    38881 tggcaatgac gcggttcagc gccccttcca gctcacgtac gttagatcgt agacgcttgg
    38941 cgataaagaa cgccacttca cccggcaaac gaatgtcgtt ttcgtcggcc tttttcatca
    39001 ggatcgccac acgggtttcc agctctggcg gttcgatcgc cacagtcagt ccccaaccga
    39061 agcgggattt caaacgatcc tcaacgccgt tgatctcttt cggatagcga tccgaggtga
    39121 gaatgatctg ttgattacct tccagcaggg cgttgaaggt gtggaaaaac tcttcctgag
    39181 atcgttcttt attagcaaaa aactgaatat cgtcgatcag cagtgcatct acggaacggt
    39241 agtagcgttt aaactcttcg atcgcgttgt tttgcagggc tttaaccatg tcctgaacaa
    39301 agcgctcgga gtgcatataa accactttgg cattcggctt gcgcgccata atgccgttac
    39361 ccaccgcatg cagcaggtga gttttaccca gacccgtgcc gccataaagg aacaacgggt
    39421 tataggcacc gccagggtta tccgccacct ggcgagccgc cgcgcgcgcc agttggttag
    39481 atttaccttc aacgaagtta tcaaacgtgt gtttgacgtt tacgttagaa cgataggtcg
    39541 gttctgccgg ggccgggacg ttatcccaac ctgagcgcgt agaaggcgca gcacgttgcg
    39601 gctgcgtttg cgccacctgt gcaggggccg cgacgttgct cgtcactgcc gcttgtggcg
    39661 tttgcgtcac cggtttggtg ccgacttcaa aacgcagctg tggggcatcc gctccgcaga
    39721 aactggttag cagtccattg atattattaa ggtacttgtc ccgtacccaa tcgaggacaa
    39781 aacggtttgg cgcgtacagg gccagcgtgt tatcgctcag ttccgcctgc aatgggcgta
    39841 tccacatact gaattctgtg gctggtaact catcctgcaa tcgggcaaga cactgctgcc
    39901 aaagcgaaag tgacacggcg gactccactc gaacaaaagt cgataatgac taaggctgaa
    39961 acattcatga ttgttgacgt acgtcgaaaa gaccctgctt gagggtgacg cacgaaccgc
    40021 tgtctgcggt tatatgcccg atcaagatcc tgcaaaacga tcgggaccgc ggatcatagc
    40081 ctaaactgcg caagagatct tctgtttctc acagattttc ccgatttatc cacaggactt
    40141 tccagacctc gctaagtgta aacgatcctg ccgcgaggcg ggcacgattt acgccgcata
    40201 ttggaaaatt taatgaccat agacaaaaat tggcttaatc gatctaataa agatccagga
    40261 cgatccttgc gctttaccca tcagcccgta taatcctcca cccggcgcgc catgctggtt
    40321 ttcactggtg tgaggtcgta cattttccct gcgaaaaggt gcggaaaagc gcggtaaata
    40381 aggaaagaga attgactccg gagtgtacaa ttattacaat ccggcctctt taatcaccca
    40441 tggcttcggt gtccatcgtt tcatttttcg gcggatatcc aataaagcca ttgaatttat
    40501 tcaagtttag gtagaaatcg ccatgaaacg cacttttcaa ccgtctgtac tgaagcgcaa
    40561 ccgttctcac ggcttccgtg ctcgtatggc tactaaaaat ggtcgtcagg ttctggcacg
    40621 tcgtcgtgct aaaggccgcg ctcgtctgac cgtttctaag taataaagct aacccctgag
    40681 tggttaagct cgcatttccc agggagttac gcttgttaac tcccagtcaa ttcacattcg
    40741 tcttccagca gccacaacgg gctggcacgc cgcaaattac cattctcggc cgcctgaatt
    40801 cgctggggca tccccgtatc ggtcttacag tcgccaagaa aaacgttcga cgcgcccatg
    40861 aacgcaatcg gattaaacgt ctgacgcgtg aaagcttccg tctgcgccaa catgaactcc
    40921 cggctatgga tttcgtggtg gtggcgaaaa aaggggttgc cgacctcgat aaccgtgctc
    40981 tctcggaagc gttggaaaaa ttatggcgcc gccactgtcg cctggctcgc gggtcctgat
    41041 agccctcatt cgggtctatc aacgcctgat tagtccgcta ctcgggccgc attgtcgttt
    41101 cactccaacc tgttcaagct acggaattga ggcattgcgc aggtttggag tgataaaagg
    41161 cagttggttg acggtgaaac gcgtattaaa atgccaccct ttacaccctg gtggtgacga
    41221 tcccgtcccg cccggaccat ttgataccag agaacactaa cgatggattc gcaacgcaat
    41281 cttttagtca tcgctttgct gttcgtgtct ttcatgatct ggcaagcctg ggagcaggat
    41341 aaaaacccgc aacctcaggc ccaacagacc acgcagacaa cgaccaccgc agcgggtagc
    41401 gccgccgacc agggcgtacc ggccagtggc caggggaaac tgatctcggt taagaccgac
    41461 gtgcttgatc tgaccatcaa cacccgtggt ggtgatgttg agcaagctct gctgccggct
    41521 tacccgaaag agctgaactc tacccagccg ttccagctgc tggaaacttc accgcagttt
    41581 atttatcagg cacagagcgg tctgaccggt cgtgatggcc cggataaccc ggctaacggc
    41641 ccgcgtccgc tgtataacgt tgaaaaagac gcttatgtgc tggctgaagg tcaaaacgaa
    41701 ctgcaggtgc cgatgacgta taccgacgcg gcaggcaaca cgtttaccaa aacgtttgtc
    41761 ctgaaacgtg gtgattacgc tgtcaacgtc aactacaacg tgcagaacgc tggcgagaaa
    41821 ccactggaaa tctccacctt tggtcagttg aagcaatcca tcactctgcc accgcatctc
    41881 gataccggaa gcagcaactt cgcactgcac accttccgtg gcgcggcgta ctccacgcct
    41941 gacgagaagt atgagaaata caagttcgat accattgccg ataacgaaaa cctgaacatc
    42001 tcttcgaaag gtggttgggt ggcgatgctg caacagtatt tcgcgacggc gtggatcccg
    42061 cataacgacg gtaccaacaa cttctatacc gctaatctgg gtaacggcat cgccgctatc
    42121 ggctataaat ctcagccggt actggttcag cctggtcaga ctggcgcgat gaacagcacc
    42181 ctgtgggttg gcccggaaat ccaggacaaa atggcagctg ttgctccgca cctggatctg
    42241 accgttgatt acggttggtt gtggttcatc tctcagccgc tgttcaaact gctgaaatgg
    42301 atccatagct ttgtgggtaa ctggggcttc tccattatca tcatcacctt tatcgttcgt
    42361 ggcatcatgt acccgctgac caaagcgcag tacacctcca tggcgaagat gcgtatgctg
    42421 cagccgaaga ttcaggcaat gcgtgagcgt ctgggcgatg acaaacagcg tatcagccag
    42481 gaaatgatgg cgctgtacaa agctgagaag gttaacccgc tgggcggctg cttcccgctg
    42541 ctgatccaga tgccaatctt cctggcgttg tactacatgc tgatgggttc cgttgaactg
    42601 cgtcaggcac cgtttgcact gtggatccac gacctgtcgg cacaggaccc gtactacatc
    42661 ctgccgatcc tgatgggcgt aacgatgttc ttcattcaga agatgtcgcc gaccacagtg
    42721 accgacccga tgcagcagaa gatcatgacc tttatgccgg tcatcttcac cgtgttcttc
    42781 ctgtggttcc cgtcaggtct ggtgctgtac tatatcgtca gcaacctggt aaccattatt
    42841 cagcagcagc tgatttaccg tggtctggaa aaacgtggcc tgcatagccg cgagaagaaa
    42901 aaatcctgat tcggtgagtt ttcgctaaaa taagggcggt cagttgaccg ccttttttct
    42961 tttcgtaggg cggataagca ccgcgcatcc gccacacaca gcaacaggaa catcatgagc
    43021 gataatgaca ctatcgtagc ccaggccacg cctccgggac gtggcggtgt tggcatcctg
    43081 cgcatctccg gcctcaaagc ccgtgaagtt gccgaaaccg tgctgggtaa actgcctaag
    43141 ccgcgctacg ccgattatct tccgtttaaa gacgccgacg gcagcgtgct cgatcagggg
    43201 attgcgctat ggttccctgg cccgaactcg ttcaccggcg aagatgtgct ggaactgcaa
    43261 ggtcatggcg gtccggtgat cctcgacctg ctgttaaaac gcattctgac cattcccggc
    43321 ctgcggattg ctcgccctgg tgagttttcc gaacgcgcgt ttcttaacga taaacttgac
    43381 ttagcccagg ccgaggcgat tgccgatctt atcgacgcca gttcggaaca ggcggcccgt
    43441 tcggcactta actcgctgca aggcgcattc tccgcacggg ttaatcatct ggtagaagcc
    43501 ctcacccact tgcgcattta cgtcgaagcg gcaattgatt tccccgatga agagatcgat
    43561 ttcctctccg acggaaaaat tgaagcccag ctcaatgacg ttattgccga tcttgatgca
    43621 gtgcgtgctg aagcacgtca gggtagtttg ttgcgcgaag ggatgaaagt ggtgattgcc
    43681 ggacgtccta acgccggtaa atcgagcctg ttaaacgcgc tggcggggcg tgaagcggca
    43741 atcgtaaccg atatcgccgg aactacgcgt gacgtgctgc gtgagcatat ccacattgac
    43801 ggaatgccgc tgcatatcat cgataccgcc gggctacgtg aagccagtga cgaagtagaa
    43861 cgtattggta tcgagcgcgc gtggcaggaa attgaacagg ccgaccgcgt gctgtttatg
    43921 gtcgatggca ccacaacaga cgccgtggat ccggcagaga tctggccgga atttattgcc
    43981 cgtctgccag cgaaactgcc gatcaccgtg gtgcgcaata aagccgatat caccggcgaa
    44041 acgctgggaa tgagtgaagt gaacggtcac gcgttaattc gtctctcggc aaggactggt
    44101 gaaggcgtgg acgtgctgcg taaccatctc aaacagagca tgggctttga caccaacatg
    44161 gaaggcggct tcctggcgcg tcgtcgccac ctacaggcgc tggaacaggc ggcagagcat
    44221 ttgcagcaag gtaaagcgca actgttaggt gcctgggcgg gcgaactgct ggcggaagag
    44281 ttgcgcctgg cacagcagaa cttaagcgaa atcaccgggg aatttacttc tgatgacctg
    44341 ctggggcgga ttttctccag cttctgtatt ggtaagtaac cgcgcttacg aagccgtatt
    44401 ctgactgtca gatgcggctt cgcttcattg ttagcactcc tgttattcct caaccctttt
    44461 tttaaacatt aaaattctta cgtaatttat aatcttaaaa aaaagcattt aatattgctc
    44521 cccgaacgat tgtgattcga ttcacattta aacaatttta gaatagacaa aattcttgag
    44581 tattatactg tagcctcgtg tctttcgagg ataagtgcat tatgaatatc ttacatatat
    44641 gtgtgacctc aaaatggttc aatatcgaca ataagattgt cgatcaccgc ccttaattta
    44701 cccttctgta gccatcacca gagccaaacc gattagattc aatgtgaacg attagtttgc
    44761 tatatcttaa ttttgccttt tgcaaaggcc atctctcgtt tatttactta ttttagtaaa
    44821 tgatggtgct tgtatatata catggcgaat taatggatat tgcagatgta atattcacag
    44881 ggatcactgt aattaaaata aatgaaggat tatgtaatgg aaaactttaa acatctccct
    44941 gaaccgttcc gcattcgtgt tattgagcca gtaaaacgta ctactcgcgc ttatcgtgaa
    45001 gaagcaatta ttaaatccgg tatgaacccg ttcctgctgg atagcgaaga tgtgtttatc
    45061 gatttactga ccgacagcgg caccggggcg gtaacccaaa gtatgcaggc agcgatgatg
    45121 cgcggcgacg aagcctacag cggcagccgc agctactatg cgttagccga gtcagtgaaa
    45181 aatatctttg gttatcaata cactattccg actcaccagg gccgtggcgc agagcaaatc
    45241 tatattccgg tactgattaa aaaacgcgag caggaaaaag gcctggatcg cagcaaaatg
    45301 gtggcattct ctaactattt ctttgatacc acgcagggcc atagccagat taacggctgt
    45361 accgtgcgta acgtctatat caaagaagcc ttcgatacgg gcgtgcgtta cgactttaaa
    45421 ggcaactttg acctcgaagg attagaacgc ggtattgaag aagttggccc gaataacgtg
    45481 ccgtatatcg ttgcaaccat caccagtaac tctgcaggtg gtcagccggt ttcactggca
    45541 aacttaaaag cgatgtacag catcgcgaag aaatacgata ttccggtggt aatggactcc
    45601 gcacgctttg ctgaaaacgc ctatttcatc aagcagcgtg aagcagaata caaggactgg
    45661 accatcgagc agatcacccg cgaaacctac aaatatgccg atatgctggc gatgtccgcc
    45721 aagaaagatg cgatggtgcc gatgggcggc ttgctgtgca tgaaagacga cagcttcttt
    45781 gatgtgtaca ccgaatgcag aaccctttgc gtggtgcagg aaggcttccc gacatatggt
    45841 ggtctggaag gcggcgcgat ggagcgtctg gcggtaggtc tgtatgacgg catgaatctg
    45901 gactggctgg cttatcgtat tgcgcaggtg cagtatctgg tcgatggcct ggaagagatt
    45961 ggcgttgtct gccagcaggc gggcggtcac gcggcattcg ttgatgcagg taaactgttg
    46021 ccgcatatcc cggcagatca gttcccggca caggcgctgg cctgcgagct gtataaagtc
    46081 gccggtatcc gtgcggtaga aattggttct ttcctgttag gccgcgatcc gaaaaccggt
    46141 aaacaactgc catgcccggc tgaactgctg cgtttaacca ttccgcgcgc aacatatact
    46201 caaacacata tggacttcat tattgaagcc tttaaacatg tgaaagagaa cgcggcgaat
    46261 attaaaggat taacctttac ctacgaaccg aaagtattgc gtcacttcac cgcaaaactg
    46321 aaagaagttt aattaatact acagagtggc tataaggatg ttagccactc tcttacccta
    46381 catcctcaat aacaaaaata gccttcctct aaaggtggca tcatgactga tcaagctgaa
    46441 aaaaagcact ctgcattttg gggtgttatg gttatagcag gtacagtaat tggtggaggt
    46501 atgtttgctt tacctgttga tcttgccggt gcctggtttt tctggggtgc ctttatcctt
    46561 atcattgcct ggttttcaat gcttcattcc gggttattgt tattagaagc aaatttaaat
    46621 tatcccgtcg gctccagttt taacaccatc accaaagatt taatcggtaa cacctggaac
    46681 attatcagcg gtattaccgt tgccttcgtt ctctatatcc tcacttatgc ctatatctct
    46741 gctaatggtg cgatcattag tgaaacgata tcaatgaatt tgggttatca cgctaatccg
    46801 cgtattgtcg ggatctgcac agccattttc gttgccagcg tattgtggat aagttcgtta
    46861 gccgccagtc gtattacctc attattcctc gggctgaaga ttatctcctt tgtgatcgtg
    46921 tttggttctt tcttcttcca ggtcgattac cccattctgc gcgatgccac cagcaccact
    46981 gcgggaacgt cttacttcca gtatatcttt atggctttgc cggtgtgtct ggcgtcattt
    47041 ggtttccacg gcaatattcc cagcctgatt atttgctatg gcaaacgcaa agataagtta
    47101 atcaaaagcg tggtctttgg ttcgctgctg gcgctggtga tttatctctt ctggctctat
    47161 tgcacgatgg ggaatattcc gcgcgaaagc tttaaggcga taatctcctc aggcggcaac
    47221 gttgattcgc tggtgaaatc gttcctcggc accaaacagc atgggattat cgagttttgc
    47281 ctgctggtgt tctccaactt agctgttgcc agttcgttct ttggtgtcac gctgggattg
    47341 ttcgattatc tggcggatct gtttaagatt gataactccc acggcgggcg tttcaaaacc
    47401 gtgctgttaa ccttcctgcc acccgcgttg ttgtatctga tcttcccgaa cggctttatt
    47461 tacgggatcg gcggtgccgg actgtgcgcc accatctggg cggtcattat tcccgcagtg
    47521 ctggcaatca aagctcgcaa gaagtttccc aatcagatgt tcacggtctg gggcggtaat
    47581 cttattccgg cgattgtcat tctctttggt ataaccgtaa ttttatgctg gttcggtaac
    47641 gtctttaacg tgttacctaa atttggctaa atccttcagg aagccagcca tttcgctggc
    47701 ttcttgcctg tcaggaaatc acttatgtcc aaatggcaac tcgcctgatt ctccttcacc
    47761 acgtatgctt tgcgtcacct tactatcagg acgctttagc ccatgtcccg ctttttgatt
    47821 tgtagttttg ccctggtttt actttatccc gccgggattg atatgtacct cgttggttta
    47881 ccgcgcatcg ccgccgatct caatgccagc gaagcgcagt tgcatattgc gttctctgtt
    47941 tatctggctg ggatggcagc tgcgatgtta tttgccggta aagtggccga tcgttcaggg
    48001 agaaagccgg tcgccatccc cggcgcggcg ctatttatta tcgcctcggt gttctgttca
    48061 ctggctgaaa ccagcacgtt atttcttgca gggcgctttc tacaggggtt gggcgcgggc
    48121 tgttgttacg tggtggcgtt cgccattttg cgcgacacgc tggatgatcg acgtcgggca
    48181 aaagtgctgt cattactcaa cggtattacc tgcatcattc cggtgttagc gccagtgctc
    48241 ggacatctga ttatgcttaa attcccgtgg cagagtctgt tctgggcgat ggcaatgatg
    48301 ggcatcgcgg tactgatgtt gtctttgttt attttaaaag aaacgcgccc tgcggccccc
    48361 gcagcttcgg acaaaccacg agaaaatagc gagtcgctgc ttaaccgttt tttcctcagc
    48421 cgtgttgtta tcaccaccct cagcgtttcg gtgatcctca ctttcgtcaa cacgtcaccg
    48481 gtattgctga tggaaatcat ggggtttgag cgcggtgaat acgccaccat tatggcgctg
    48541 accgctggcg tcagcatgac cgtttcattc tccacgccat ttgcgctggg aatttttaag
    48601 ccacgtacgt tgatgatcac ctcgcaggtg ttattcctgg cggcggggat cacccttgcc
    48661 gtttcacctt cccatgcggt ttctctgttt ggtatcacac tgatttgcgc cggtttctcg
    48721 gtcggttttg gcgtagcgat gagtcaggcg ttagggccgt tttcattacg cgcgggcgta
    48781 gccagctcga ccttaggtat tgcgcaggtt tgcggttcgt cactgtggat ttggctggcg
    48841 gcggtggttg gtatcggcgc atggaatatg ctgatcggga ttctgattgc ctgtagcata
    48901 gtgagcctgt tgctgattat gttcgtcgcg cctggacgcc ccgttgccgc tcatgaagaa
    48961 atccatcacc acgcttgatc tcaatttgct gctctgtctg caactgctga tgcaggagcg
    49021 cagcgtgacc aaagcggcga agcggatgaa cgtaacccct tcggcggtga gtaagtcgct
    49081 ggcaaagtta agagcatggt ttgacgaccc gctctttgtg aactcaccgc tggggctgtc
    49141 gcccacaccg ctaatggtca gcatggagca aaatctggcg gagtggatgc aaatgagcaa
    49201 cctgctgctg gataaaccgc accaccaaac gccgcgcggc ctgaagtttg agctggcggc
    49261 ggaatcaccg ctgatgatga tcatgcttaa tgcgctgtcg aaacagatct accaacgtta
    49321 cccgcaggcg accatcaaat tacgtaactg ggattacgat tccttagatg ccattactcg
    49381 tggtgaagtg gatattggtt tttccggtcg cgaaagccat cctcgctcgc gggagctgtt
    49441 aagctcgcta ccgttagcca ttgattatga agtgctgttt agtgatgtgc cctgcgtctg
    49501 gttacgcaaa gatcatccgg cactgcatga aacgtggagt ctggacactt ttttgcgtta
    49561 tccgcatatc agcatttgct gggaacagag cgatacctgg gcgctggaca atgtgttaca
    49621 ggagctgggg cgcgaacgca cgattgctat gagcctgccg gaattcgagc agtcactgtt
    49681 tatggcagcg cagcccgaca atctgctact ggcgaccgcg ccgcgctact gtcagtacta
    49741 caatcaactc caccaactgc cgttggttgc gcttcctctc ccgtttgacg aaagccagca
    49801 aaaaaagctg gaagtccctt ttaccctgct ttggcataaa cggaacagcc ataatccgaa
    49861 gatcgtctgg ttacgggaaa ccattaaaaa tctttacgcg tcgatggcat aaccgaatcg
    49921 tatgaaacgc gaccctattt cacaataaaa tgtaaaaatt ttgtaataaa cttgtctgaa
    49981 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttgtattca
    50041 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
    50101 ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat
    50161 tccccttcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
    50221 catgctgtat ggcattggcg aattgccgga aattgtcacc ctgccgaaag gtaaaccggt
    50281 tttcagcgat aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt
    50341 ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg
    50401 gtttcatagc ccacacgcgc ccgataacca caccttttca agcaatgaat cgctatggat
    50461 cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc atcacgctgc gccgaagcgt
    50521 gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcagc tgctgcccat
    50581 tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggaaga
    50641 cgtgctggtg tggtccgtgg cggtcacgcc cacgattgaa aagctcagtg tctgggagtt
    50701 agatggcaaa cacggctgga aaagcctgcc ggatattcac agccgcgcca acaatcctac
    50761 cagccggatg atgcgttttg cccagctctc taccgtgaag gctttttcgc caaattgata
    50821 gacaaccaca ggagtcatca tgtctgaaaa attgaaggtg gttacgttac tggggagcct
    50881 gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag
    50941 catggaagtc aatgcgttac cttccattgc cgacattccc ttgtatgacg ctgacgtaca
    51001 gcaggaagaa ggttttccag caacggtaga agctctggcg gaacagatcc gtcaggctga
    51061 cggtgtggtg attgtcacgc cggaatataa ctactcggtt ccgggtgggc tgaaaaatgc
    51121 catcgactgg ctttcccgcc tgccggatca accgctggcc ggtaaaccgg tgttgattca
    51181 gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct
    51241 ggttttcctc gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa
    51301 caaagttgat ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg
    51361 gcaattgacc gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc
    51421 agtaatcatg catggcgggt ttttaatgcg ctatcgattt taatgagcgt cgataaatac
    51481 aatcttcagg ataaacatca gcgcaacgat gattacgcac gggctaaggt cacgcagacg
    51541 cccggtacca atcttcatca cgcagtagga gataaagccc agcgcaatac cttcggtaat
    51601 cgaaaagctg aacggcatca tcacggcggt aataaacgcc ggaacagatt cagtaagatc
    51661 ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc
    51721 agctgcagcg tagcctggca ccattcccgc cagcggcgac agaaagataa ccagcaggaa
    51781 cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga
    51841 ggactcaata taagccgtaa cggaagaagt accgataaac gaaccggtca cggaagagat
    51901 actgtcgaca tacagcgcct gcttcatgcg cgggaattta cccttctcat ccgccaggcc
    51961 tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaggttga ccagcatgaa
    52021 agagaaaatc acccctgcca gcccgaggtt aaacgacccg gctaaatcta catggcccac
    52081 gacagtcatt acgctcggcg gcgcagaaac gatgccattg tagtggacat cacccagcat
    52141 ccagcccagc agcgttgtca ccacgatgga aaccagcacc gccgcgtgaa tgttgcgcga
    52201 ggccagaata gcgatgatga agaagccgag gatccccagc agcacgctgt gagaagtcag
    52261 gttaccgatg ctgaccaacg tttccgggtt agcgacaatc acacctgcat tcttcagtcc
    52321 catcatacca atgaacagac cgataccgct ggtgatgccc acacgcagac ttaccggaat
    52381 gttagcaatc atccagtaac gaacgcggaa aatcgtcagt aacagcagac caatcgcgcc
    52441 ccagaagatt gcgcccatcc cgacttgcca cggcaagccc atagcctgta caacgacaaa
    52501 agcgaagaat gcattcaggc ccatagcggg tgccagtgca actggcaggt tagcaaacag
    52561 tcccatcata atactgccga atgcagcaat cagacaggta gtaacgaaga cggcgctggt
    52621 atccatgcca gcaacgccaa gaatttgcgg gttaacaaaa acgatgtaaa ccatcgtcag
    52681 gaaggtggta aaaccggcga tcacttcggt ccgtgccgtc gtgccatgtt cgcgcagttt
    52741 aaacacgcgt tccagcatcc cctggccaga agcctgggtt gtgtgttgtt gactcattat
    52801 ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatga gacaatatgg
    52861 acggctgaag ataacttttt ttcattgtat ttgcttatac ggcaacgatt gcgtcatgcg
    52921 aattggcatg acgaaaacgt taaactaacg ataaaggaaa gacatgtccc agatagaagc
    52981 ggtatttttc gactgcgacg gtacgctggt cgacagtgaa gtcatttgct cccgcgcata
    53041 tgtaacgatg tttcaggaat ttggtattac gctcgatcct gaagaggtat tcaaacgttt
    53101 caaaggtgta aaactgtacg aaattatcga tattgtttcc cttgaacatg gtgttacgtt
    53161 agcgaaaaca gaagctgaac acgtttaccg tgcagaagtc gctcggctgt tcgattcaga
    53221 actggaagcc atcgaagggg ctggagcgct cctgtcagcg atcactgcgc caatgtgtgt
    53281 ggtatctaac ggcccaaata acaaaatgca gcattctatg ggcaagctga atatgttgca
    53341 ctacttcccg gataaactgt tcagcggcta cgatattcag cgctggaagc cagacccggc
    53401 gttaatgttc catgcggcaa aagcgatgaa tgtaaatgta gaaaactgca ttctggttga
    53461 tgactcagtt gccggtgcgc aatctggtat cgacgcaggt atggaagtgt tctacttctg
    53521 cgccgacccg cacaataagc cgatcgttca cccgaaagtc accaccttta cccatctttc
    53581 gcagttaccg gaactgtgga aagcgcgtgg ttgggatatt acggcatagt tcttcacact
    53641 cccttcactt accccgctta aattggcgct caaaggtaag taaagggagt ttgatatgtc
    53701 tgtttcacgt cgggtaatac atcacggact ttattttgcg gttttaggac cgttaattgg
    53761 tgttctgttt cttgtcctct acatattctt cgcaaaagaa ccgctggttc ttttgcgaag
    53821 aatacaggta cttccattgt ttctcttatt gtcgattact acgggagtta ttcctgcatt
    53881 gttaaccggc gtgatggtcg cctgtctgcc ggaaaagatc gggtcacaga aaaattatcg
    53941 ttgtctggct ggtggcatag gtggcgtcgt tatcaccgag atctattgtg cagttattgt
    54001 acatattaag ggcatggctt cctcggagtt gtatgaaaac attctttctg gtgacagtct
    54061 cgttgtccgc atcattcctg cattgctggc aggtgtggtg atgagcagaa tcattacccg
    54121 tctgcccgga ttggatattt catgtcctga aacagactct ttaagttaag cgggatactt
    54181 tatctttggg ctactcaaaa gcagacagga tgttactatg actcaaaata tcaggccgtt
    54241 accccaattc aaatatcatc ccaagccact ggaaacaggc gcatttgaac aggataaaac
    54301 cgtagagtgc gattgctgtg aacaacagac gtcagtttat tactcgggtc ccttttattg
    54361 cgttgatgaa gttgaacatc tctgtccgtg gtgtattgcg gacggttctg ctgctgaaaa
    54421 gtttgcaggt agttttcagg atgatgccag catagaaggt gttgaatttg agtatgatga
    54481 agaggacgaa tttgccggta ttaagaacac atatcctgat gaaatgctga aagagttggt
    54541 tgaacgcacg ccaggttatc atggatggca acaggaattc tggcttgcgc attgtggcga
    54601 tttctgtgct tttatcggct atgtgggctg gaatgatata aaagatcgcc tcgatgaatt
    54661 tgccaacctt gaagaagatt gtgagaattt cggtattaga aattctgatc tagctaaatg
    54721 cctgcaaaag cgtggtgatt gtcagggtta tctcttccgc tgtctccact gcggcaagct
    54781 gagactgtgg ggtgattttt cgtagttatt taaataatga gaacaggccg gagcgtaatt
    54841 catacatccg gccttatttc ttcagcttaa ccgagcgcta attctgcggc agcggcttta
    54901 tcagcgatta ccatcagcga aggatgcagc tgcaacacgg aagctggaac atcttcggta
    54961 accggacctt gcagcacatt tttcagtgcc tgcgctttac ctgcgccact gacaattatc
    55021 agcaagtttt tcgccgccat gatgcttttc ggccccatcg tcacgtagct gtcaggcact
    55081 aacgaaaagt cgccgcccaa ctcaccatgc gccacaatat caaccatctc tccctggatc
    55141 gggaactcca cggtttgctc atggaaatgg gtcgtattcg gcaaattgcc acaaaaatga
    55201 ccatctgcac ctaatcccag caccaccaaa tccaggccgc cttcacgcgc cagtttctga
    55261 tcatgctcgc ggtagttatc aatagtgagc ttctggatat tctcttcttt gatccccgca
    55321 ggggtgaaaa acagattacg cagattggta atcgttacac cttctccctc tttgccgcga
    55381 aagggaattt catcgaaatt atagaaatag cagttatcat accagggctt acctttaacc
    55441 agggtggtga ggtattcata catgcctttg ggcgtgctac cggcggtaat tgccaggtta
    55501 acacgacgcg tcttcgacat ataaccaagc agatggtgtg ccgcgacacg gctcatttcc
    55561 tggtaatctt cggtaatgat taatttcatc ttagtgcctt attttattta aataatttct
    55621 gtacgaaggc tgcataggca ggccgccaga catccatttc atggttcagg ccgggatatt
    55681 cctggtaatc aaacttaatt tttttctgct caagctcagt tttcagcccg gcgatatcct
    55741 tgccggttac gacatcttta tcaccaacga ccacagtaaa attacgtagt tgctggttaa
    55801 tagctgccgg atcgttcagc cgggccgcga caccttcatc cggtacggtt gtcgtggtaa
    55861 caccactgaa tgtggccagc cagccaaagc tttccagatg attcattccg gaaaccagcg
    55921 cctggtaccc gccttgtgaa agccctgcca gcgcgcgacc atcggcatct ttacggacat
    55981 taaaacgctt gctaatcagc gggataatat cgttcatcag ttcgcgatct gccgctttag
    56041 cattcagcgg ataaaagact ttacgtcttt cctgaggcac gaaatcttcg ggaataatgc
    56101 ccttcgcatc ggtttcggta tccgggatca ccaccagcat cggtttaatt ttcccttcag
    56161 ccagcaggtt atccatgatt tgcgggatac gcccctgatc gatagcggaa cgtccggtat
    56221 caccaaaacc gtgatagaaa tagagcactg gcaaaggctc gcccatgccg gtgtatcctg
    56281 gcggggtcca gacatacatc tgacgttcag attgcaatgc gttggagtgg taagttatgg
    56341 cgatcaaatc accatgcgca acagaacgcg tatccagata actgcctggc accagaatca
    56401 tactggagtt aacctggcgc tgaggcttag tcattgcggt gcctgtatca atgctgcgta
    56461 caccatcaac attgaaaaaa tactcgtaca ggttgccttt caggataggt gtgcgccacg
    56521 accagactcc tgcttcgtct ttggtcatcg ggtgaatatt gtccggaacc ggaacaccca
    56581 ccactacaga gacatttttt gccccagggg caaagtagcg aaaggtcacg ctgttatcgg
    56641 cattgacctg agtcacatac tgtttcaccg ggatagtggg tgccgggctg gctggcatat
    56701 ccgctgctat ggcccactga gcagaaatac cgctggcaat agccagcgtt aaagcagcaa
    56761 tttttatatt catcgtaata ttcctttcat taagttccat taccaccaga tttcagcctg
    56821 ggcaccgaca gcgaactggt cgtctttatt gtcttcgaag gtgaagccat ccagttcgtt
    56881 ttccagggct ttgatatacg tggcgtagaa acgaatttcc ggacgcgagg tcaacatact
    56941 ggtattgact ttaaaggtat ggaagagcgt ggttttataa ccagactcat taaatttatt
    57001 actattcgca tctttgtttt gctgggtgaa atagcccagt tcaacacctg tctggttata
    57061 ttggtcccaa atataggccg ggcgaacaac cgcacgaata gattcgaaat cagagtgtgc
    57121 gcctgtttcg tagctataaa tatcgttacc gaaggagtaa acaatcgcgt tagccacaat
    57181 aaaatgatcg ccgatatagg cttcgccctg tgaagtcaga cgaactgctg ttccgccggt
    57241 gtgatcacca taataacgac cattaaaggt ggtaaatgga cttgcgccag cataacggcc
    57301 aaagttactg gcgatagagt tattcgcaac caggaaggag aattcgttga agccaccttt
    57361 atcaaatttc tgcgttaaag atgtgccgaa catccaggta tctttccagt cataataccc
    57421 gttattatcc tgattatctt tttccgatgc gctttcgttt gccgtgacat aacgaccact
    57481 caccattaag gtcgctttat cccataacgg gatatcttta tagcgtaaat caatagtatt
    57541 ggtattaatc tgctgtttgt tttgcaggct gcgatcgtaa tcatcgacat cttcgcgaac
    57601 cagcgcgata tcaattttac ccggaccgac tttccagttt tccagaccta cacccgctgc
    57661 ggcatcagta cgctgcgttt tccagtccag catctggatt tcaattttcg gcgcaccgtg
    57721 tttacccacc cagaaatcag cctctggcgc aaagggcagg aaacctttgg tggtaacgta
    57781 catatcggag aactgcataa agttctcgcc accggcgtta tcgccaaacc agccggtaga
    57841 gtactgctga ccaacgttac catccatcat cacaacggca tcaacccgtt tgccgttttc
    57901 gttgtagaca cgttgtttta actgcaaatc aaaccagccg gagtattcgt taccaaagcg
    57961 gcccagagaa ccaatcgccc atgatttagg tgaaccatga gaggcggtcc cccaaccaga
    58021 acggtaatag ccgttgtaac taaagccaat ttcatctttc acaaacttac tgaaatcttt
    58081 cagagtcatg gaagaataaa tcgagcctgt ctcactggca tttttctctt catttttgac
    58141 cagtacagca tcaggttttg cggcactact ggtcgggaac ggattggcgg catacccttg
    58201 atcattcgta cttacgctac gattcaccgt cgctggcgta tattttttct tctcttcatc
    58261 tttatacttt ttgagttcgc tttgcgtttc tcttaacgct ttttctaata actcaaggcg
    58321 ttgttccacc gttaatgatt cagccaatga ttgtgcagaa aaggctaacg gtgccattaa
    58381 taagacggca gaggtaataa gatttcgtct aaacataata tccctttatg gtgcaaagaa
    58441 agaattaacg tatcgcatcc agactattct gaatgcgacg ataattaagg tgctttattg
    58501 gttatttttt taatgacagc ccccgcgttt tgatcacttc tgcataccat ccgaagcttt
    58561 tcttacgcgt tcttgtgagg cttccttcgc cattatcatc acgatccaca taaataaagc
    58621 cgtagcgctt agacatttgt gaatgagatg cactgactaa atcaattggc ccccaactgg
    58681 tgtaccccat aatatccaca ccatcggcaa tcgcttcatt tacctgtacc aggtgatcgt
    58741 ttaaataggc aattcgataa tcgtcctgta tcgaaccatc cgcttcaacg ctgtcttttg
    58801 cgcctaatcc gttctcgaca ataaataacg gtttttgata acgatcccaa agcgtattta
    58861 acagaacccg taatccaacc ggatcaattt gccaccccca ctctgaactt ttcagatgcg
    58921 gattggggat catattcagt atgttgccct gcgcattttt attaatgctt tcgtcgtggg
    58981 aaacacaacc agtcatgtaa taactaaaag agatgaaatc gacggtatgt tttaaatctt
    59041 ctgcgtcact ttcagtcatc tcaatggtga tattgtggtc gcggaagaaa cgctgcatat
    59101 agccgggata ctggccacgc gcctgaacat caccaaagaa catccagcgc cggttctctt
    59161 ccatggcctg caacatatcc tgtggctggc aggtgagggg gtaaaccagc ccaccgagaa
    59221 gcatattgcc gattttcgct tcggggagca ggctatgaca ggctttaact gcccgcgcac
    59281 tggcaaccag ttgatggtgg atagcctgat aaacttccgc ctcgccactc tcttctgcca
    59341 gccccacgcc cgtgaatggc gcgtgtaacg acatgttgat ttcattaaac gtcagccata
    59401 acgccacttt atgttggtag cgagtaaaga ccgtgcgggc gtaatgctcg aagtgatcga
    59461 tgaccgctcg attagcccaa ccgccgtagt ttttcaccag cccatatggc atttcgtaat
    59521 gggataacgt taccagcggc ttgatccccg cctgcgccat ttcatcaaac agccgatcgt
    59581 aaaacgctaa ccccgcttca ttcggttcga cttcgtcgcc ctgagggaaa attcgcgccc
    59641 aggcaatgga aatacgcaga caggtgaagc ccatctcggc aaataacgcg atatcttccg
    59701 ggtaacggtg ataaaaatcg atggcgacat ctttgatatt ctctttcccc aggatgcgcg
    59761 gttccatttt tcccattacg ccatgaggct gtaaatctga ggtcgagatc cctttgccat
    59821 cttcctgcca ggcaccttcc acctgattgg cagctgttgc gccaccccaa agaaatgttt
    59881 ctggaaatgc tttcataatt aactcctttt atcgttagcg aatgatggat aacagcggtt
    59941 cacctgcgct tatctgcgcc gtgccgtggg gtaatacgtc cgtaaaatca tcgctattac
    60001 tgattaatac cggcgtcgtc agatcaaatc cggcctcgcg aatagcaggg atatcaaaag
    60061 aaatcagccg atcgcctgta ttgaccttgt cacccacgtt gacgtgagcg gaaaagaatt
    60121 tgccgtccag ttttacggtg tcgataccga catgaatcag gatctccaca ccatcatctg
    60181 actcaatgcc aatggcgtgt aatgtggcga acaacgaagc aattcgaccc gcaaccggag
    60241 aacgcacttc accaaccgag ggcagaatgg cgataccttt acccaacagg ccactggcaa
    60301 acgtggtatc agcgacgtga atgagcggca caatctctcc cgtcatcggt gaacagatac
    60361 cgccctgctc aggtggtgta ataacctctg gtgttttctc ttgcggggca ccctgcgctg
    60421 gctgacgttt agcggtgatg aaatgaagca tcaccgtacc gacaaatgcg caaccgatgg
    60481 caatgacacc gccaataacg ctggcccaga cggtgaaatc aattcccgtt gacgggatgg
    60541 tttgcatgaa ggtgaaaata cttggcaaac caaaggagta gactttcgtt tgcgcgtagc
    60601 caatgatggt ggcccccaaa gccccactga tacaggcgat aacaaagggg tacttacgcg
    60661 gcaggttgac gccatatacc gctggttcgg tgataccaaa cagactcgtc aacgccgctg
    60721 atcccgccac cacttttttc tgcgcatcgc gttcgcagag gaagacgccg agcgccgccc
    60781 cgacctgcgc cataatggcg ggcattaaca gcgggatcat ggtgtcgtag cccagcacgg
    60841 tgaagttatt gatacacagc ggcaccaggc cccagtgcag tccgaacatg acgaagattt
    60901 gccagaagcc gcccattacc gcgcccgcaa atgcaggaac cgcctgataa agccagagat
    60961 aaccggcggc aatcagttcg cttatccagg ttgatagcgg ccccaccagc agaaaggtga
    61021 cgggtgtgat aaccatcaga catagcaatg gtgtgaagaa atttttgatt gccgacggta
    61081 accacgcatt aagtcggcgt tccagaatgc tgcacaacca ggcagaaaaa ataatgggaa
    61141 taaccgatga cgagtaattc aacaatgtga ccggaatacc caggaaatcc agccccagcg
    61201 catccgcttt ttgcccgttc tcgaaagcag tcagaattaa tggatgcact aacgctccac
    61261 caatcaccat ggcagtaaat ggattaccgc cgaagcgttt ccccgcggtg tatcccagga
    61321 ttatcgggaa gaaccaaaac aaggcatcac tggcgctgaa taaaattaaa taagtaccac
    61381 tttgttcggt cgtccactga aaagtgagcg ccagagccag catacctttc aggatcccgg
    61441 ttgccgccat caaaccgatc agaggcgtaa aaatacctga aataacataa acaaagcggt
    61501 ttagcagatt acctttatca tcattttccg gtgcctgttg cgctttttcg tcaaggcctg
    61561 ccacactgtt aaccgccagg aagacatcgg ccacatggtt acctatgacc acctgaaact
    61621 ggccaccgct ttccaccacc ataataatac cgggggtctt tttcagtacc tctgcttgcg
    61681 ctttgctttc atcctttaac ttaaaacgta atcgcgttgc gcaatgcatc agactcacaa
    61741 tgttatctgc gcccccgact cctgcgacta tttttctggc taactccgtc ataacttgcc
    61801 ctctaccgct ttgcggcaaa actcaaaaaa aaacctgaaa aaaacggcct gacgtgaatc
    61861 aagcaatttt ttcaggtttt gcccgcttag tgcggtaaca atcctttact cagtaataat
    61921 atttcagtgt tctttgtgca cgcgctctat atttatggct aaaaacataa tctctgcggg
    61981 tgagatttta cgttgatact gcaaaccaat aaaaatggcg atccgctccg cacattgcca
    62041 tgcttgcggg taattttgtt ttactgcttg ttgtaatgac tcatcactat cgttaattga
    62101 agcatgtcca agaatacgcc aggataaaaa cttcagatgc gtaaccagtc gctgataact
    62161 caagctttct tcctggtaat taaggctgaa ctgaaatttt attaattgca gcatttcgcg
    62221 cattaattgc gtaacacctg caacatcctc catatttccg ctcatttggg cactgaccag
    62281 atgcatggca ataaagccca cttcatcttt cggtaactgc acgcccaacc gtttatcaat
    62341 aatggttaac gcttcttccc ctagctggaa ctctttcggg taaagccgct ggatatccca
    62401 cagcagcggg ttgggcagta acacgttttg ctgaaagcgt ttaatcgcaa actggcaatg
    62461 gtcagttagc gagatataaa tactgtcctg taattttccc aagcgctcct gcgctaaaga
    62521 gataatacga tcacaggttg ccatcacctc aagaggaata tgacttaaga gttcgcttaa
    62581 tcgcccgttc agttcatgac tgctcaaggc atactctttt tctattccac ttgagttaat
    62641 tctttcgcca gcgcgttttt gaaagccaat tcctcgcccc atgacgactt tttcccgctg
    62701 ttgatcatca ataaccacca caacattatt gttgagaatt ttggtgattt gcatgttcat
    62761 agcaaggacc ttttttataa acaaaaaaac ccgacttcac cagtattctc tggttatgtc
    62821 aggttttgcc tgcgaatgca gtaacaatcc agtcatttat taatggtttt tataacgaac
    62881 atccaggttc ggaaattaat ttaattgcgt gcagagaaaa tagcaatgcg ctattgataa
    62941 aaatatgacc atgctcgcag ttatt
//
LOCUS       DAFNBJ010000007        62074 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000007 DAFNBJ010000000
VERSION     DAFNBJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.007"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..579
                     /locus_tag="L2X49_000461"
     CDS             <1..579
                     /locus_tag="L2X49_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBN4795603.1"
                     /translation="GCGASLARLAQKGIYIAAVVMTTGNSGTDGIIDRHEESRNALKI
                     LGCHQTIHLNFADTRAHLQLNDMISALEDIIKNQIPSDVEIMRVYTMHDADRHQDHLA
                     VYQASMVACRTIPQILGYETPSTWLSFMPQVFESVKEEYFTVKLAALKKHKSQERRDY
                     MRHDRLRAVAQFRGQQVNSDLGEGFVIHKMIL"
     gene            589..1785
                     /locus_tag="L2X49_000462"
     CDS             589..1785
                     /locus_tag="L2X49_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBN4795604.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYSQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            1763..2344
                     /locus_tag="L2X49_000463"
     CDS             1763..2344
                     /locus_tag="L2X49_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBN4795605.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2346..3119
                     /locus_tag="L2X49_000464"
     CDS             2346..3119
                     /locus_tag="L2X49_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HBN4795606.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3304..3579
                     /gene="frmR"
                     /locus_tag="L2X49_000465"
     CDS             3304..3579
                     /gene="frmR"
                     /locus_tag="L2X49_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HBN4795607.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3614..4723
                     /gene="frmA"
                     /locus_tag="L2X49_000466"
     CDS             3614..4723
                     /gene="frmA"
                     /locus_tag="L2X49_000466"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HBN4795608.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            4816..5649
                     /gene="fghA"
                     /locus_tag="L2X49_000467"
     CDS             4816..5649
                     /gene="fghA"
                     /locus_tag="L2X49_000467"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBN4795609.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(5774..6313)
                     /locus_tag="L2X49_000468"
     CDS             complement(5774..6313)
                     /locus_tag="L2X49_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HBN4795610.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6415..7626)
                     /gene="mhpT"
                     /locus_tag="L2X49_000469"
     CDS             complement(6415..7626)
                     /gene="mhpT"
                     /locus_tag="L2X49_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HBN4795611.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(7802..8815)
                     /gene="dmpG"
                     /locus_tag="L2X49_000470"
     CDS             complement(7802..8815)
                     /gene="dmpG"
                     /locus_tag="L2X49_000470"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HBN4795612.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADMVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(8812..9762)
                     /gene="mhpF"
                     /locus_tag="L2X49_000471"
     CDS             complement(8812..9762)
                     /gene="mhpF"
                     /locus_tag="L2X49_000471"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HBN4795613.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPKSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(9759..10568)
                     /gene="mhpD"
                     /locus_tag="L2X49_000472"
     CDS             complement(9759..10568)
                     /gene="mhpD"
                     /locus_tag="L2X49_000472"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HBN4795614.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10578..11444)
                     /gene="mhpC"
                     /locus_tag="L2X49_000473"
     CDS             complement(10578..11444)
                     /gene="mhpC"
                     /locus_tag="L2X49_000473"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HBN4795615.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11462..12406)
                     /gene="mhpB"
                     /locus_tag="L2X49_000474"
     CDS             complement(11462..12406)
                     /gene="mhpB"
                     /locus_tag="L2X49_000474"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HBN4795616.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12408..14072)
                     /locus_tag="L2X49_000475"
     CDS             complement(12408..14072)
                     /locus_tag="L2X49_000475"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HBN4795617.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTTGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMLQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14149..15096
                     /gene="mhpR"
                     /locus_tag="L2X49_000476"
     CDS             14149..15096
                     /gene="mhpR"
                     /locus_tag="L2X49_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HBN4795618.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLAARPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            15173..16255
                     /gene="lacI"
                     /locus_tag="L2X49_000477"
     CDS             15173..16255
                     /gene="lacI"
                     /locus_tag="L2X49_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HBN4795619.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16378..19452
                     /gene="lacZ"
                     /locus_tag="L2X49_000478"
     CDS             16378..19452
                     /gene="lacZ"
                     /locus_tag="L2X49_000478"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBN4795620.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRVGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPTRIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19504..20757
                     /gene="lacY"
                     /locus_tag="L2X49_000479"
     CDS             19504..20757
                     /gene="lacY"
                     /locus_tag="L2X49_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HBN4795621.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTVNNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            20823..21434
                     /gene="lacA"
                     /locus_tag="L2X49_000480"
     CDS             20823..21434
                     /gene="lacA"
                     /locus_tag="L2X49_000480"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HBN4795622.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLLKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFKDYKVESSV"
     gene            complement(21537..22718)
                     /gene="cynX"
                     /locus_tag="L2X49_000481"
     CDS             complement(21537..22718)
                     /gene="cynX"
                     /locus_tag="L2X49_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HBN4795623.1"
                     /translation="MRNHQKTFTILLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(22724..23194)
                     /gene="cynS"
                     /locus_tag="L2X49_000482"
     CDS             complement(22724..23194)
                     /gene="cynS"
                     /locus_tag="L2X49_000482"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HBN4795624.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23225..23884)
                     /gene="cynT"
                     /locus_tag="L2X49_000483"
     CDS             complement(23225..23884)
                     /gene="cynT"
                     /locus_tag="L2X49_000483"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HBN4795625.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23994..24893
                     /gene="cynR"
                     /locus_tag="L2X49_000484"
     CDS             23994..24893
                     /gene="cynR"
                     /locus_tag="L2X49_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HBN4795626.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24933..26216)
                     /gene="codA"
                     /locus_tag="L2X49_000485"
     CDS             complement(24933..26216)
                     /gene="codA"
                     /locus_tag="L2X49_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HBN4795627.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26206..27465)
                     /gene="codB"
                     /locus_tag="L2X49_000486"
     CDS             complement(26206..27465)
                     /gene="codB"
                     /locus_tag="L2X49_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBN4795628.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27797..29683)
                     /gene="prpE"
                     /locus_tag="L2X49_000487"
     CDS             complement(27797..29683)
                     /gene="prpE"
                     /locus_tag="L2X49_000487"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HBN4795629.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29723..31174)
                     /gene="prpD"
                     /locus_tag="L2X49_000488"
     CDS             complement(29723..31174)
                     /gene="prpD"
                     /locus_tag="L2X49_000488"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HBN4795630.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31208..32377)
                     /gene="prpC"
                     /locus_tag="L2X49_000489"
     CDS             complement(31208..32377)
                     /gene="prpC"
                     /locus_tag="L2X49_000489"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HBN4795631.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32422..33312)
                     /gene="prpB"
                     /locus_tag="L2X49_000490"
     CDS             complement(32422..33312)
                     /gene="prpB"
                     /locus_tag="L2X49_000490"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HBN4795632.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALVVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            33550..35136
                     /gene="prpR"
                     /locus_tag="L2X49_000491"
     CDS             33550..35136
                     /gene="prpR"
                     /locus_tag="L2X49_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HBN4795633.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35234..35509)
                     /locus_tag="L2X49_000492"
     CDS             complement(35234..35509)
                     /locus_tag="L2X49_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBN4795634.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35656..36327
                     /locus_tag="L2X49_000493"
     CDS             35656..36327
                     /locus_tag="L2X49_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBN4795635.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGNAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36344..36589)
                     /locus_tag="L2X49_000494"
     CDS             complement(36344..36589)
                     /locus_tag="L2X49_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795636.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(37001..37816)
                     /locus_tag="L2X49_000495"
     CDS             complement(37001..37816)
                     /locus_tag="L2X49_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795637.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(38059..39108)
                     /gene="yahK"
                     /locus_tag="L2X49_000496"
     CDS             complement(38059..39108)
                     /gene="yahK"
                     /locus_tag="L2X49_000496"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HBN4795638.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(39124..39261)
                     /locus_tag="L2X49_000497"
     CDS             complement(39124..39261)
                     /locus_tag="L2X49_000497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795639.1"
                     /translation="MGNVAALFHSGSRHVGAGSGGLNIGYIQWTRFAAMTYQASPNTH
                     S"
     gene            complement(39485..40867)
                     /locus_tag="L2X49_000498"
     CDS             complement(39485..40867)
                     /locus_tag="L2X49_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBN4795640.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(40877..41827)
                     /locus_tag="L2X49_000499"
     CDS             complement(40877..41827)
                     /locus_tag="L2X49_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HBN4795641.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(41970..43388)
                     /locus_tag="L2X49_000500"
     CDS             complement(41970..43388)
                     /locus_tag="L2X49_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBN4795642.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(43388..44935)
                     /gene="fdrA"
                     /locus_tag="L2X49_000501"
     CDS             complement(43388..44935)
                     /gene="fdrA"
                     /locus_tag="L2X49_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBN4795643.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(44925..45788)
                     /locus_tag="L2X49_000502"
     CDS             complement(44925..45788)
                     /locus_tag="L2X49_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBN4795644.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDEQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALVLFIPGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHSFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(45828..46433)
                     /locus_tag="L2X49_000503"
     CDS             complement(45828..46433)
                     /locus_tag="L2X49_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HBN4795645.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDLTCLNPFLMSCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            46691..47188
                     /locus_tag="L2X49_000504"
     CDS             46691..47188
                     /locus_tag="L2X49_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBN4795646.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNSSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            47280..48212
                     /locus_tag="L2X49_000505"
     CDS             47280..48212
                     /locus_tag="L2X49_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4795647.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(48254..49342)
                     /gene="pdeL"
                     /locus_tag="L2X49_000506"
     CDS             complement(48254..49342)
                     /gene="pdeL"
                     /locus_tag="L2X49_000506"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HBN4795648.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFVSEWVMKAGG"
     gene            complement(49837..49911)
                     /locus_tag="L2X49_000507"
     CDS             complement(49837..49911)
                     /locus_tag="L2X49_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795649.1"
                     /translation="MCDILLNVLNIVFIGIAIILVIIC"
     gene            complement(50217..52250)
                     /gene="betT"
                     /locus_tag="L2X49_000508"
     CDS             complement(50217..52250)
                     /gene="betT"
                     /locus_tag="L2X49_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HBN4795650.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            52379..52966
                     /gene="betI"
                     /locus_tag="L2X49_000509"
     CDS             52379..52966
                     /gene="betI"
                     /locus_tag="L2X49_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HBN4795651.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            52980..54452
                     /gene="betB"
                     /locus_tag="L2X49_000510"
     CDS             52980..54452
                     /gene="betB"
                     /locus_tag="L2X49_000510"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HBN4795652.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSALQGQKIWASMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            54466..56136
                     /gene="betA"
                     /locus_tag="L2X49_000511"
     CDS             54466..56136
                     /gene="betA"
                     /locus_tag="L2X49_000511"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HBN4795653.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            56349..57017
                     /locus_tag="L2X49_000512"
     CDS             56349..57017
                     /locus_tag="L2X49_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795654.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
                     DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIR
                     LEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(57260..57955)
                     /locus_tag="L2X49_000513"
     CDS             complement(57260..57955)
                     /locus_tag="L2X49_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBN4795655.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVILFSAAERGRSLSLLP
                     EYSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(57948..59375)
                     /locus_tag="L2X49_000514"
     CDS             complement(57948..59375)
                     /locus_tag="L2X49_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBN4795656.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFARTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(59386..60105)
                     /locus_tag="L2X49_000515"
     CDS             complement(59386..60105)
                     /locus_tag="L2X49_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBN4795657.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELFTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(60633..61487)
                     /gene="rclR"
                     /locus_tag="L2X49_000516"
     CDS             complement(60633..61487)
                     /gene="rclR"
                     /locus_tag="L2X49_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HBN4795658.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRLAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            61713..>62074
                     /locus_tag="L2X49_000517"
     CDS             61713..>62074
                     /locus_tag="L2X49_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBN4795659.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHG"
BASE COUNT        14905 a        16659 c        15700 g        14810 t
ORIGIN      
        1 ggctgtggcg catcgcttgc tcgtcttgcg caaaaaggaa tttatatcgc agccgtggtg
       61 atgactaccg gcaactctgg cacagatgga ataatcgatc gccatgaaga atcgcgcaac
      121 gccctaaaga tattagggtg ccaccaaact attcatctta attttgctga cacccgcgct
      181 catttacagc tcaatgatat gatttccgcc ctggaagaca tcattaaaaa tcaaattcct
      241 tctgatgttg aaatcatgcg ggtatatacc atgcatgatg ccgaccgcca tcaggatcat
      301 ctcgctgttt atcaagcttc aatggttgcc tgccgcacta ttccacaaat tctcggctac
      361 gaaaccccaa gtacctggct ttcatttatg cctcaggttt ttgaatccgt taaagaagaa
      421 tatttcacag tcaaacttgc tgcattaaaa aaacataaaa gccaggaacg acgcgattat
      481 atgcgtcatg atcgcctgcg tgcagttgca caatttcgcg ggcaacaggt caatagcgat
      541 ctgggtgaag gctttgtaat tcataaaatg attctttgag caaattgcat gaaaacctgg
      601 atatttatct gtatgtccat agcaatgttg ctatggtttt taagtacgct aagacgtaaa
      661 cccagtcaaa agaaaggctg tattgacgcc attatacctg cgtataacga aggcccgtgt
      721 ctggcgcagt cactggataa tctactgcgt aacccttatt tttgccgggt aatttgcgtt
      781 aacgacggct ccacggacaa taccgaagcg gtcatggcgg aagtcaaacg caaatggggc
      841 gaccgctttg ttgccgtcac gcaaaaaaat accggtaaag gtggtgcgct gatgaatggc
      901 ctcaattatg ccacctgcga ccaggttttt ttaagtgatg ccgacaccta tgttccgccc
      961 gatcaagacg gaatgggcta tatgctggca gaaattgagc gcggtgctga tgccgtaggc
     1021 ggcattccct ctactgcgtt gaaaggcgcg gggctgttac cgcacatccg cgcgaccgta
     1081 aagttgccga tgattgttat gaagcgcacg ctacagcagc tcctgggcgg cgcaccgttt
     1141 attatcagcg gtgcctgcgg aatgttccgc actgatgtat tgcgtaagtt cggtttctca
     1201 gatcgtacta aagtcgaaga ccttgatctc acctggacat tagtggcaaa cggctaccgt
     1261 attcggcagg cgaatcgctg catcgtatac tcacaggaat gcaacagccc gcgtgaggag
     1321 tggcgtcgct ggcggcgttg gattgtggga tacgcggtct gtatgcgcct gcataaaaga
     1381 cttttattta gccgcttcgg tatcttcagt atatttccta tgctgttggt tgtgatttat
     1441 ggcgttggga tttatctcac tacctggttt aatgaattca tcaccaccgg gccgcatggt
     1501 gtggtgttgg caatgtttcc gcttatctgg atcggcgtag tttgtgttat tggtgctttt
     1561 agcgcctggt ttcatcgttg ctggttgttg gtgcctttag cgccgctttc cgttgtgtat
     1621 gtattattag cttatgccat ctggattatt tatggactta ttgccttttt tactggacgc
     1681 gaacctcagc gcgacaaacc cacccgctat tccgcactgg tggaagcgtc aaccgcttat
     1741 tcccaacctt ctgtcacagg aactgaaaaa ctatctgaag cttaatgtta aagagaaaaa
     1801 tattcagatt gcagaccagg taattattga tgaaactgca ggtgaagttg ttatcggcgc
     1861 gaatacccgt atttgtcatg gtgccgttat tcagggtccg gtagtgattg gcgcaaactg
     1921 cctgataggt aattatgcgt ttattcgtcc tggcacaata atcagcaatg gtgtaaaaat
     1981 tggttttgcc accgaaatta aaaatgcggt tattgaagcg gaagcaacga ttggtccgca
     2041 atgttttatt gccgactcgg tagttgcaaa ccaggcatat ttgggcgcac aagtacgtac
     2101 cagtaatcat cgtctggatg aacagcctgt gtctgttcga actccagagg gaattatcgc
     2161 taccggatgc gataaattag gttgttatat cgggcagcgt tcacgccttg gcgtacaagt
     2221 tataattttg cctgggcgaa ttatttcccc gaacacgcaa cttggcccgc gcgtgattgt
     2281 agaacgtaat ttacctgccg gaacttattc actccgacaa gaacttatcc gtacaggaga
     2341 ttaaaatgat aaaacggacg ttattagcgg cggccatttt tagcgcattg cccgcttatg
     2401 ccgggttaac ttccattacc gcgggctacg attttaccga ttattctggc gatcatggca
     2461 accgtaattt agcgtatgct gaactggtgg cgaaagttga aaacgcaacg ctgcttttta
     2521 atctttccca ggggcgtcgt gattatgaaa ctgaacattt caatgccact cgcggtcagg
     2581 gtgcggtctg gtataaatgg aataactggc tgacaacccg aacgggtatt gcctttgcgg
     2641 ataatacgcc ggtctttgcc cgccaggatt ttcgtcagga tattaacctg gccctgttgc
     2701 caaaaacgct tttcacgacc ggttatcgct acactaaata ttacgatgat gtcgaagtcg
     2761 atgcctggca aggcggcgta tcactctata ctggcccggt gatcaccagc taccgctata
     2821 cccattatga ctccagcgat gcaggtggta gttatagcaa tatgatttcc gtgcgtctga
     2881 atgacccgcg cggcactggt tatacgcaac tatggctaag ccgcggaaca ggcgcttaca
     2941 cctatgactg gacgccagaa acacgctacg gcagcatgaa gagcgtcagt ttgcaacgta
     3001 ttcaaccgct gactgagcaa cttaatctcg ggctgacggc aggtaaagtg tggtacgaca
     3061 ccccaaccga tgattataac ggtctgcaac ttgcagccca tctgacctgg aaattctgat
     3121 tccttctgcc gcccgctatc cggggcggcc ttccctgccg attagccccc cctttcctct
     3181 ttgttttccg accacattca ccggataaat tttattctcc agtgttatat actatagggg
     3241 ggtatgcatt gaaatatagc atacccccct atagtatatt gcgtgcagat gatgaggtgc
     3301 gaaatgccca gtactccgga agagaagaaa aaggtcctta ctcgagttcg tcgtattcga
     3361 gggcagattg atgctctgga acggtcgctg gagggtgatg ccgaatgccg tgccatactc
     3421 caacagatcg ctgccgttcg gggcgcggct aacgggctga tggcagaagt gcttgaaagc
     3481 catatccggg aaacgtttga ccgaaatgac tgctacagcc gcgaagtcag ccaatccgtt
     3541 gacgacacta ttgaactggt tcgagcctat cttaaatagc tgaatctatt accatattga
     3601 ggaagagcga gagatgaaat cacgtgctgc cgttgcattt gctcccggta aaccgctgga
     3661 aatcgttgaa attgacgttg caccaccgaa aaaaggtgaa gtgctgatta aagtcaccca
     3721 taccggcgtt tgccataccg acgcatttac cctctccggg gatgacccgg aaggtgtatt
     3781 cccggtggtt ctcggtcacg aaggggccgg cgttgtggtt gaagtcggtg aaggcgtaac
     3841 cagcgtcaaa cctggcgacc atgtgatccc gctttacacc gcggagtgcg gcgagtgtga
     3901 gttctgtcgt tctggcaaaa ctaacctctg tgttgcggtt cgcgaaaccc agggtaaagg
     3961 cttgatgcca gacggcacca cccgtttttc ttacaacggg cagccgcttt atcactacat
     4021 ggggtgctct acattcagtg aatacaccgt ggtcgcggaa gtgtctctgg ccaaaattaa
     4081 tccagaagca aaccatgaac acgtctgtct gctgggctgt ggcgtgacca ccggtattgg
     4141 cgcggtgcac aacacagcta aagtccagcc aggtgattct gttgccgtgt ttggtcttgg
     4201 cgcgattggt ctggcagtgg ttcagggcgc gcgtcaggcg aaagcgggac ggattatcgc
     4261 tatcgatacc aacccgaaga aattcgatct ggcgcgtcgc ttcggtgcta ccgactgcat
     4321 taacccgaat gactacgaca aaccgattaa agatgtcctg ctggatatca acaaatgggg
     4381 tatcgaccat acctttgaat gcatcggtaa cgtcaacgtg atgcgtgcgg cgctggaaag
     4441 tgcgcaccgc ggctggggtc agtcggtgat catcggggtg gcaggttctg gtcaggaaat
     4501 ctccacccgt ccattccagt tggtcactgg tcgcgtatgg aaaggttccg cgtttggcgg
     4561 cgtgaaaggt cgttcccagt taccgggtat ggttgaagat gcgatgaaag gtgatatcga
     4621 tctggaaccg tttgtcacgc ataccatgag ccttgatgaa attaatgacg ccttcgacct
     4681 gatgcatgaa ggcaaatcca ttcgaaccgt aattcgttac tgatttcccg caggtatacc
     4741 ccgtccactt cagacggggt tcttaatact ctccccgggc agccgtccgg gggattaacc
     4801 gtgagataat gactgatgga actcattgaa aaacatgcca gctttggcgg ctggcaaaat
     4861 gtgtatcggc attattccca atcactgaaa tgtgaaatga atgtcggcgt ctatctccca
     4921 ccaaaagccg cgaatgaaaa attgccggtg ctgtactggc tttcgggcct gacctgcaac
     4981 gagcagaatt tcattactaa atcggggatg cagcgttacg cggctgagca caacattatt
     5041 gttgttgcgc cggacaccag tccgcgaggc agtcatgtcg cagatgccga ccgttacgat
     5101 ctcgggcaag gtgccgggtt ttacctgaac gcaacgcaag cgccgtggaa tgaacattac
     5161 aaaatgtatg actatatccg caacgagctg ccggatttag tgatgcaaca ttttccggca
     5221 acgaccagaa agtctatctc tggtcattct atgggcgggc tgggcgcgct ggtgctggcg
     5281 ttacgtaacc cagatgaata tgtcagcgtc tcggcgtttt cgcccattgt ctccccatcg
     5341 caagtaccgt ggggacagca agcctttgcc gcatatcttg gtgaaaataa agatgcctgg
     5401 ttggattacg atccggtgag tcttatttca caaggtcaac gcgttgcgga aatcatggtt
     5461 gatcaggggt tgagtgatga tttttacgcg gaacagctgc ggactccaaa tcttgaaaag
     5521 atctgccagg agatgaatat caagacgtta atccgttatc acgagggtta cgaccacagc
     5581 tattattttg tctccagttt tattggcgag catattgcct accacgccaa taaactgaat
     5641 atgcgttgat aatagtgcac gactgccgga tgcggcgtga acgccttatc cgcctacatt
     5701 tcgcccgcag gccgtaggca tgataagacg cgcatagcgt cgcatcaggc atctgcgcac
     5761 gacttatcta tgtttaccac atcaaatcat caggcacttt gaagtcggca tacggatcgt
     5821 cttcatcctg ctcttcggca ctgagcgcgc tgtgcaacac tatactgctg gcatcacgct
     5881 gagctatttt atcggcaacg ctggcgggaa taatggcgta ttcaccttcg ctattgttat
     5941 cgaccaacaa gcgagcaatc gccagacgac cattaatcaa ctgcgcctgt gtgagcttat
     6001 cgacaaaaat cttcttaatc agattgccgt cagtgaagtt aaaaccaata tcgccattgg
     6061 caatggtgat tcggttcatt tcaatgagct gcttcacctg agctttatat tctttcgcca
     6121 acgcagcttg tttttgttgc tcgctaagct gtttatcacg ctcaagctgt gcctttttat
     6181 tttcttctac cgccgcccga gcttcacgcg cctgaacgcg cgattttttc gccgttctct
     6241 ccaccttcgc cgcttttttg ctggtgacta atcctgcttt gagcaactgc tcttgtaagg
     6301 taagttttgc catctttgtt tccaaacccg ctgaataatt tttagcgagt atacctgtaa
     6361 acgccgtggg tgtattgggg gcaagtccct ttcgcccctg ctaaataagg cgcgtcaggc
     6421 atcggcacac ggctgcattc ttgatctccg actcatcaaa ataaacaccg ccaacccagc
     6481 aacaagaata cccggtgcag aagcggccat tacgccgacc gtgccagtgc ctaatgccag
     6541 cattttcccg gccagtaacg gaccgctcat agcccccaga cgccctaccg ccacggctgt
     6601 tcccacacct gttgcgcgga tctgcgaact gtaaaacaac ggtgccaggg catacaaaac
     6661 gctttgccca cctgtcgcaa acaaccccgc gacaaatccc gccagcaaca taccgttaaa
     6721 tgacgacacc gttccaagcg ccagcagcga agctaacatg ccgctataaa tcagtagcga
     6781 catggttact ggacgcagct tatccatcaa tgcgcccaac attaacgtcc cgcttgccgc
     6841 ccccatttgc agggcaaaca tcacccctgc cgcctgcgat ggctggaatc cttgctccac
     6901 caaaagtagc ggtagccagt tgatcaacat ataaaccacc agcagagtga agaaataaca
     6961 caaccacagc agcagcgtcg cggttgccgt ttctggcgca aataaggcac gcagtggtgg
     7021 cgcagcctgt ttttcgccag cgaaaaccgc tgactccggc agccagcgca ttaatagcgg
     7081 caccagaatc aacggcacca caccacctac ccaaaacacc gtttgccatg ctaagtttgc
     7141 ccccgcgaaa cccagtgtcg ccgccagcgc cgcgccaatg ggaacaccgc aatacatcag
     7201 gctcactgcc gtcccacgaa aacgtggacc cgcggcttca gacgtcagag cgataagatt
     7261 cggcaacgcc gcccccagcc cgacaccggt catcagccgc gcaaagacca gtgaggggaa
     7321 atcccaggca atcgccgttg ccagtgagaa caaaccaaac agcgcaactg agccaatcaa
     7381 aatacgctta cgaccataac ggtctgccag catcccgcca accaacgcgc cgggtagcaa
     7441 accgagtatt ccggcgctaa atatccagcc catttgcatt ttatcgagtg cgaaagcctg
     7501 ggcgatgcca cccgccgcaa tgccagccgc ctgaagatcc agcccttcca tcagagcgac
     7561 caaaaaacaa agcccgatgg tcagcatcag gcgggatgaa gatgatgaag gggtacgagt
     7621 cgacataaga tgaacctcat tgcgagtgca ggtgaatcgc gactgataca ttgccagccg
     7681 cgtaatcaga actatttaat gactgcctac gggcgaaatg taggccggat aaggcgttca
     7741 cgccgcatcc ggctgtcgtg ctgggatggg catacaaaat gcacgttatc ggcagtcatc
     7801 attatttgtt gttgcgcaga tccagcgcca cgtcgacgat catatcctcc tggccgccaa
     7861 ccatccggcg tttgcccagc tcaacgagaa tatccaccgc acttaagcca taacgcgccg
     7921 ccgccgtttc acagtgacgc aggaagctcg agtaaacacc agcgtatccc agcgccagcg
     7981 tttcgcgatc gactcgtacc ggtcgatcct gcaacggacg caccaggtcg tcggcggcat
     8041 ccattaacgc atagagatcg gtcccatgct gccagcccag tttatccgcg gcggcaataa
     8101 acacttccag cggtgcgtta cctgcgcccg cgcccattcc cgcgaggctg gcgtcgattc
     8161 ggtcgcagcc ctcttccacc gccgcgatag agttcgccac gccaagactc aggttatggt
     8221 gagcgtgcat gccagtttgc gtttctggtt tcagctctgc tttcagggcg cggaaacggt
     8281 cacggatatc gctcatgttc atcgcaccgc cagaatccac cacataaata caggtcgcac
     8341 cgtagccttc catcagcttt gcctgcttgg cgagattctc cggcgtggtc atatggctca
     8401 tcatcagaaa accaacggtg tccattccga gctcgcgggc atactgaata tgctgggcgg
     8461 aaacatcagc ttcggtacag tgcgttgcca cacgaaccac ccgcgcgcca gcctgccagg
     8521 catttttcag atcgtgaata gtgccgattc ctggcagcaa caacgtcgcg attttggcgt
     8581 gcttcaccat atccgccgct gcttcaatcc attcaaggtc gctatgtgcg ccgaaaccat
     8641 agttaaagct ggaaccctgc aaaccgtcgc catgggccac ttcaatcgaa tccacgcggg
     8701 catcgtccag tgctttggca atctggcgaa cgttttccag cgaatactga tgacgaatgg
     8761 cgtgcatacc gtcacgcaac gtgacgtccg agatataaag ttttttaccg ttcatgccgc
     8821 ttctcctgcc ttgcgcgcca gtgactgggc cattttttcc gctgtcgcca gcgcactgga
     8881 agtcataatg tcgaggttgc ccgcataggc aggcagataa tgcgctgcgc cttcgacttc
     8941 cagccagacc gctgttttca gtccggagaa ttgccccacg cccggtaaat tgaccggttt
     9001 atcctgcggg ataacttcaa actgcacgcg ctgtttcagg cgataacccg gtacgtaagc
     9061 ctgcaccgcc tcagccattt cattgattga ggcttcgata tcatcttgtg aagcttcgtc
     9121 gctcaataca tacaccgtgt cacgcatcat caacggtggc tctgctgggt taagcacaat
     9181 aatcgccttc ccttttgccg cgccgcccac cacttcaatg gctcgggaag tggtttccgt
     9241 aaattcatcg atattggcac gcgtgccagg tccggcagat ttactggcga tagaagcgat
     9301 aatttcggcg taatgaacac gcgccacgcg tgaaactgcc gccaccattg gaatggtggc
     9361 ctggccgccg caggtgacca tgttgacgtt cagttgatcg acgttcgcct cgaggttaac
     9421 caccggcacg cagtaagggc cgatggcagc aggcgtcagg tcaattaagc gaatatccgg
     9481 tttcgcttcg cgtaaagcgg catcgttttt cacatgcgcg cccgcgctgg tcgcatcaaa
     9541 tacaatgtcg atatcagcaa attcaggcat gttcatcagc ccgatcaccc cttcatgggt
     9601 ggtggcgacg cccatacgtc tggcacgcgc cagaccgtcg gacttaggat caatgccgac
     9661 catcaccgcc atctccagat gctgaccgtg acgcaaaatt ttaatcatca gatcggtgcc
     9721 aatgttgcca gaaccgataa tggcgacttt acgcttactc atgacagact tccttttggg
     9781 gctgcgcttg aaaatgtcgc agcaactgaa cctatgcctt caatatgggc ttcaaaacga
     9841 tcgcccgcat tcaccgccac catcgggcct aatgccccgg taagaatgat atctccggcg
     9901 cgcagcggtt cacccagact ggccattttg cgtgccagcc agacggcggc attaagcgga
     9961 tgtcccaggc attcgctgcc gcgcccgctg gaaacctctt cgttattacg cgtcatcttc
    10021 atggcgcagt ttttcaggtc taaccccgcc ggacgttgcg ccggaccgcc gatgacatac
    10081 accccgcagg aagcgttatc tgccacggta tcgacaaact gaatcgacca gtcgcgaatg
    10141 cggctcccca ccacttccag cgccggaagt acccattcaa tggcgttata cagttcgtcg
    10201 aaggtgatat cagttgcggg taaatcgcgg ttcaacacca gtgcgatctc cgcttcaatg
    10261 cggggttgca gaacacggga aaaaggaatg atttcgttat cgccataaca catgtcggca
    10321 aataacgtcc caaaatccgg ttgatcaacg cccagttgtt gttgcacttt cggatgcgtc
    10381 aggcccactt tacgccctac cacgcgacgc ccctgcacaa cgtcatattg cacatttatg
    10441 tgctgaatgg cgtaggccgc ttcagcgtta tcgataccaa tcagatcgcg cagcggcgca
    10501 atcgcttcgc cctgctctgc ggcgcggcgt aaatccgccg ccagttgctc aagagtatgc
    10561 ttcgtcatga ccattcctta agggcgtgcg aggaaattca gcaccaattg attgaaagcg
    10621 tcggcatgtt cccactgcgc ccagtgacca cagtcgcgga agatatgcag ttcagaaccg
    10681 gcaatgccgg acagcagacg cagacccgca tccatcggca caaagcggtc gttgcgcccc
    10741 cagacaatca gggtttgcgc tttgatttcc gccagacgtg ggccaaaatc cgggaactgt
    10801 ttcggattag cttccaggct cttaacgaag ttttccaggt gatcgcggcg cgacagcata
    10861 ttattcaggc gcgcttcaaa cagggcgtcg gtcaaatcgc tggtatcaaa aacgaagata
    10921 tccatcatca gcttcaggtt ttcgatagtc ggctgacgat aaagctgatt cagtcgctta
    10981 ataccttcgg ttggcatcgg cgtaaacaaa ctcatgccgc ccgtaccgcc gcccatcagc
    11041 accagtttgc cgacgcgctc cggccattta agggtgaacg ccacagaact atggcccccc
    11101 atcgagttgc ccagcaggtg gattttggcg atatccagtt gatccaccac gcttttcagg
    11161 attcgtgcat taagatccga tcgtgaacca ctattaacga tcgaatcgct tttgccccaa
    11221 cccggacaat ccagcaggat tacccgatag cccgcctcta ccagcggatc gatattgcgg
    11281 ctgaagttcg cccagccagt agcacccggg ccggaaccat gcagcaggac aacggtttcg
    11341 tcgccttgtc cgcagtcatt aaaatggatg cgcagcgttt tacccgcttc ttctacattc
    11401 agaaaacggc tggtggcggc ttcggtttgt ggctgataac tcatcatctt ctcctgcata
    11461 ttcagttctc tgttctggcg cttaacgagc caaatccggc aatccactcc gggattgggc
    11521 ggtaataacg cccttcgcta cgccagttgc caaatgtaga aatagcggca aaagcggcga
    11581 cccaggtttt gatttcatgt gtcgactttc cggcaatggc ggaaagctct tcgttactga
    11641 cggcatccag ttcctgtatg cgtccctgct ccagcaaagt catgaactgg ttatcccaaa
    11701 tcgggttgag cggatgcaga gttctctgat cctcaacaaa cttctcagcg gcgctaatca
    11761 cccgttgctg acgcaattcg cgctcactgg cgggtaaatc tttcccgctc cccaacagac
    11821 ggtcgcgcat atgggcatcg gctttcgcca gttcgggcac cggcggctga tgggaaagcc
    11881 caccggaacc caggaacagc acgcgtttat tgagagtgct ggtgaaacgt ccaatggctt
    11941 cacccaacat gcgggtacgc tggaaaccgg gcagcggcgt ggcgacaccg ttgatgaaca
    12001 caggcagaac tggcacctta tccagcccac cgagcaggaa ctccagcggc tgggcgaacc
    12061 cgtggtccac ctgcatacag taagaaacgg caagatcgat cccgctcttc atgacagcat
    12121 gcgcacaggc ctccgccagc tccacaggca cgggcagctc tcctgccgca ctgccgaaat
    12181 caccaattgc cgtcgctcca acgcctaaac agaacggtgg catcacgtca tagaaaaagc
    12241 cgttgtagtg atctggcgca aacagcacca ccagttcagg ggagaaagcc gcaatacgct
    12301 cgcgggcgct ggcaatcacg ccattgacct catcgagtac ctcttgcgcc gggtcgacat
    12361 accccaccaa cggcgagtgg gaaagacagt gaagataagc gtgcatatca ggctaccttt
    12421 tcgacagaaa cgtcggcatc agggcgggtc agcgtcatca ccgacgccag tttattcagc
    12481 gtattgccca gggtttgcgg aatggcggtg gcggcaacaa agcgatccgg gcgcatcacc
    12541 accagcgaag cattgtgttg cgcgaaccag ctacgcagac gaccttgcgt atcgcccacg
    12601 cgtagcacgc cgtcgtggtt atcctgtgcg gtatgaattt gcacttccgg caccacctga
    12661 atgaagcgtg tgcccaacgc gcgccactgc tggatttgct cgtcgctcat cccccacagt
    12721 ggattgcatc cccagccaat taccgcgaag ttcgcgccaa tcgcgttatc gagcagcgtc
    12781 acgtcgccgt tttccagcgt gactttcggc tgaataaaca tcttgccgac cggagagtgc
    12841 ttcgcttcgc cctcacgtac cagcgcaccg ccgtaatatt gcagcatcgg cttgaagcgc
    12901 atttcgagga agtagcgttt tactggcggc agataattca gtagccagga aacgccgtca
    12961 cgtaacgtac cctgccagcg tttcggcgga gccagcacgt tgcccgtcgt cacggacagg
    13021 tcaatcatcg ctttggcgtg atcgcgacgt tcttgttgat aggtatcgag cagcgcatcg
    13081 cgggctttcc cctggataac caacgccagt ttccatgcga ggttaaaggc gtcgcgcata
    13141 ccactgttat agccctgccc ctgccatacc ggcatgatgt gcgcggcatc gcccgccagc
    13201 agtacgcgat caatacggaa acgttgcgcc agtcgcgcgt tgtgggtgta gacacgctgg
    13261 cgaatcaatt caacattgtc cggattaggc agcactttgc ttaacagctt gcgcatattt
    13321 tgcggctcac gcagctgttc ttcggtttct cccggcatca ccataaattc aaagcgacgt
    13381 accgcatgag gcagcgcggc agaaacatac gggcgcaccg gatcgcaaca taaatagata
    13441 tgcggcgtac ttaacggatc gttggcgata tccaccacaa tccactgatt tggcgcagtt
    13501 ttaccttcaa acggcacatt cagggtgcga cgaacaaaac ttgccccacc atcacaggcc
    13561 accagccact gggctttgac tatttcccgc tgcccttctg ccgttttcag gtgcaaggtc
    13621 acttcgtcat tttgctggct gaaggcctcc agctcgcggg aaaacaagca gcgcacatta
    13681 ggaaaacgcg acaccccttc cagcatcacc gcatcgacct gcggctgaat aaaggcgtta
    13741 cggcgcggcc agccaaattc atcggtcatt ggctgaatat cagcaaaaca gcggcctttc
    13801 ggggtgagaa aacgcatcgc gtgccacggc gtagtgtgcg gcagaacatt ctcgaccagg
    13861 ccaaccgact gcatggtgcg cagcgcctcg tcatcaatac caatcgcacg cgggtagtcg
    13921 atcaacttat cgagtttctc caccaccagc acgtcaatgc ccatctgacc gagatagttc
    13981 gccatcatca gcccaaccgg accggcacca gcgatcgcca cctgaacgct atggttaaca
    14041 gcaggctgga tgtcagggtg ttgtattgcc atttcagtac ctcacgactc ggacaaaatg
    14101 ccgttgcgcg cacagtacgg cgcaacttat tttgttaaaa acatgtaaat gattttttat
    14161 tgtgcgctca gtataggaag ggtgttttcg gctacaatca aaacatgccc gaatgtgcac
    14221 caggtgcaca acgttgtttt aactatagaa atgtcaatta atatgcagaa caatgagcag
    14281 acggaataca aaaccgtgcg cggcttaacc cgcggtctaa tgttattaaa tatgttaaat
    14341 aaacttgatg gcggtgccag cgtcgggctg ctggcggaac tcagcggcct gcatcgcacc
    14401 actgtgcggc gactgctgga gacgctgcag gaagagggat atgtccgccg tagcccctcc
    14461 gatgacagtt ttcgtctgac catcaaagtg cggcaattaa gcgaaggatt tcgtgacgaa
    14521 cagtggattt ctgcactggc ggccccgctg ctgggcgatc tgttgcgcga agtggtatgg
    14581 ccaacagatg tgtccaccct ggatgttgat gcaatggtgg tacgcgaaac cacccaccgt
    14641 ttcagccgct tatcctttca ccgggcaatg gtcgggcgac gtttaccgct cctgaaaacc
    14701 gcctcgggcc tgacctggct ggccttttgc ccggaacaag aacgcaagga attaatcgaa
    14761 atgttagccg cccgccccgg tgatgactat caactggcac gagaaccgtt aaagctgcaa
    14821 gccattctgg cgcgcgcgcg caaagagggt tacggacaga actaccgcgg ctgggatcag
    14881 gaggagaaga ttgcctctat cgccgtaccg ctgcgcagtg aacaacgggt gattggctgt
    14941 ctgaatctgg tgtatatggc gagcgcaatg accattgaac aggcagcgga aaagcatctt
    15001 ccggcgctac aacgggtagc aaaacagatc gaagaagggg ttgaatcgca ggctattctg
    15061 gtggccggac ggcgaagcgg cgtgcattta cgttgacacc atcgaatggc gcaaaacctt
    15121 tcgtggtatg gcatgatagc gcccggaaga gagtcaattc agggtagtga atgtgaaacc
    15181 agtaacgcta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttctcgcgt
    15241 ggtgaaccag gccagccacg tttctgcgaa aacgcgagaa aaagtggaag cggcgatggc
    15301 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
    15361 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
    15421 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
    15481 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
    15541 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
    15601 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
    15661 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
    15721 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
    15781 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
    15841 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
    15901 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
    15961 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
    16021 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
    16081 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
    16141 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    16201 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    16261 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    16321 cggctcgtat gttgtgtgaa attgtgagcg gataacaatt tcacacagga aacagctatg
    16381 accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    16441 gttacacaac ttaatcgcct tgcggcacat ccccctttcg ccagctggcg taatagcgaa
    16501 gaagcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
    16561 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
    16621 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
    16681 accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
    16741 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
    16801 attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacggtcg ctgggtcggt
    16861 tacggtcagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgtcgga
    16921 gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct ggaagatcag
    16981 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca
    17041 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg
    17101 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta
    17161 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
    17221 gagcgtggta gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg
    17281 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc
    17341 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
    17401 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat
    17461 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
    17521 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg
    17581 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg
    17641 gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta
    17701 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
    17761 gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct
    17821 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
    17881 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
    17941 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
    18001 gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc
    18061 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
    18121 aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag
    18181 ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca
    18241 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
    18301 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt
    18361 aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt
    18421 gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta
    18481 gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg
    18541 cgtctggcgg aaaacctcag tgtgacgctc ccctccgcgt cccatatcat cccgcaacta
    18601 accaccagcg aaacggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgt
    18661 cagtcaggcc ttctttcaca gatgtggatt ggcgatgaaa aacaactgct gacgccgctg
    18721 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
    18781 attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
    18841 gcgttgttgc agtgctcggc agatacactt gccgacgcgg tgctgattac gaccgctcac
    18901 gcgtggcagc atcagggaaa aaccttattt atcagccgga aaacctaccg gattgatggt
    18961 agtggtcaaa tggcgattac cgttgatgtt gaagtagcga gcgatacacc gcatccgaca
    19021 cggattggcc tgacctgcca gctggcgcag gtcgcagagc gggtaaactg gctcggatta
    19081 gggccgcaag aaaactatcc cgaccgcctt actgcggcct gttttgaccg ctgggatctg
    19141 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
    19201 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc
    19261 cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
    19321 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
    19381 ccgtcagtgt cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg
    19441 tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc
    19501 attatgtact atttaaaaaa cacaaacttt tggatgttcg gtttattctt tttcttttac
    19561 ttttttatca tgggagccta cttcccgttt ttcccgattt ggctacatga catcaaccat
    19621 atcagcaaaa gtgatacggg tattattttt gccgctattt ctctgttctc gctattattc
    19681 caaccgctgt ttggtctgct ttctgacaaa ctcgggctgc gcaaatacct gctgtggatt
    19741 attaccggca tgttagtgat gtttgcgccg ttctttattt ttatcttcgg gccactgtta
    19801 caatacaaca ttttagtagg atcgattgtt ggtggtattt atctaggctt ttgttttaac
    19861 gccggtgcgc cagcagtaga ggcatttatt gagaaagtca gccgtcgcag taatttcgaa
    19921 tttggtcgcg cgcggatgtt tggctgtgtt ggctgggcgc tgtgtgcctc gattgtcggc
    19981 atcatgttca ccgtcaataa tcagtttgtt ttctggctgg gctctggctg tgcactcatc
    20041 ctcgccgttt tactcttttt cgccaaaacg gatgcgccct cttctgccac ggttgccaat
    20101 gcggtaggtg ccaaccattc ggcatttagc cttaagctgg cgctggaact gttcagacag
    20161 ccaaaactgt ggtttttgtc actgtatgtt attggcgttt cctgcaccta cgatgttttt
    20221 gaccaacagt ttgctaattt ctttacttcg ttctttgcta ccggtgaaca gggtacgcgg
    20281 gtatttggct acgtaacgac aatgggcgaa ttacttaacg cctcgattat gttctttgcg
    20341 ccactgatca ttaatcgcat cggtgggaaa aatgccctgc tgctggctgg cactattatg
    20401 tctgtacgta ttattggctc atcgttcgcc acctcagcgc tggaagtagt tattctgaaa
    20461 acgctgcata tgtttgaagt accgttcctg ctggtgggct gctttaaata tattaccagc
    20521 cagtttgaag tgcgtttttc agcgacgatt tatctggtct gtttctgctt cttcaagcaa
    20581 ctggcgatga tttttatgtc tgtactggcg ggcaatatgt atgaaagcat cggtttccag
    20641 ggcgcttatc tggtgctggg tctggtggcg ctgggcttca ccttaatttc cgtgttcacg
    20701 cttagcggcc ccggcccgct ttctctactg cgtcgtcagg tgaatgaagt cgcttaagca
    20761 atcaatgtcg gatgcggcgc gagcgcctta tccgaccaac atatcaggac ggaatgatcg
    20821 caatgaacat gtcgatgaca gaaagaataa aagcaggcaa gctatttacc gatatgtgcg
    20881 aaggcttacc tgaaaaaaga cttcgtggga aaacgttaat gtatgagttt aatcactcgc
    20941 atccatcaga agttgaaaaa agggaaagcc tgcttaaaga aatgtttgcc acggtagggg
    21001 aaaacgcctg ggtagaacca cctgtctatt tctcttacgg ttccaacatc catataggcc
    21061 gcaattttta tgcaaatttc aatttaacca ttgtcgatga ctacacggta acaatcggtg
    21121 ataacgtact gattgcaccc aacgttactc tttccgttac gggacaccct gtacaccatg
    21181 aattgagaaa aaacggcgag atgtactctt ttccgataac gattggcaat aacgtctgga
    21241 tcggaagtca tgtggttatt aatccaggcg tcaccatcgg ggataattct gttattggcg
    21301 cgggtagtat cgtcacaaaa gacattccac caaacgtcgt ggcggctggc gttccttgtc
    21361 gggttattcg cgaaataaac gaccgggata agcaatatta tttcaaagat tataaagttg
    21421 aatcgtcagt ttaaattata aaaattgcct gatacgctgc gcttatcagg cctacaagtt
    21481 cagcgatcta cattagccgt atccggcatg aacaaagcgc aggaacaagc gtcgcatcat
    21541 gcctctttga cccacagctg cggaaaacgt gctggtgcaa aacgcagggt tataatcatc
    21601 agcccaacga cgcacagcgc atgaaatgcc cagtccatca ggtaattgcc gctgatacta
    21661 cgcagcacgc cagaaaacca cggggcaagc ccggcgatga taaaaccgat tccctgcata
    21721 aacgccacca gcttgccagc aatagccggt tgcacagagt gatcgagcgc cagcagcaaa
    21781 cagagcggaa acgcgccgcc cagacctaac ccacacacca tcgcccacaa gaccggcaat
    21841 tgcagcggca accagataaa gccgcagaac cccaccagtt gtaacaccag cgccagcatt
    21901 aacagtttgc gccgatcctg atggcgagcc atagcaggca tcagcaaagc tcctgcggct
    21961 tgcccaagcg tcatcaatgc cagcaaggaa ccgctgtact gcgcgctggc accaatctca
    22021 atatagaagg cgggtaacca ggcaatcagg ctggcgtaac caccgttaat cagaccaaag
    22081 taaacaccca gcgtccacgc gcgaggagtg aaaaccacgc gaaccggagt ggttgttgtc
    22141 ttgtgggaag aggcgacctc gcgggtgctt tgccaccacc aggcaaagag cgcaacaacg
    22201 gcaggcagcg cccaccaggc gagtgtttga taccaggttt cgctatgttg aactaaccag
    22261 ggtgtcaggg cggcaccaag cccaccgccg cccatcagag ccgcggacca tagccccatc
    22321 accagtggcg tgcgctgctg aaaccgccgt ttaatcaccg aaggcatcac cgcctggatg
    22381 atgccgatcc ccaccccacc gagcagtgcg ctgctaagca gcagcgcact ttgggggtaa
    22441 agctcacgca tcaatgcacc gacggcaatc agcaacaaac tgatggcgac gctgcgacgt
    22501 tcgctgacat gctgatgaag ccagcttccg gccagcgcca gcccgcccat ggtaaccacc
    22561 ggcagagcgg tcaacagggc agccacgcta aagctcattc cgctcgcctg gcgcaattgc
    22621 ggtagcagtg gcccgacgga ggtgagcagt ggtcgcatat taagaccaat cagcaccaga
    22681 accagcagta tcgtgaacgt cttttgatgg ttgcgcatgg ctgtcagaac ggtttggtcg
    22741 gcagatattt accatctaag gtgatgaccg cacgttcgcc accttccggg tccgccactt
    22801 tcttaacgtc gagtttgaag ttaatcgcgc tgataatgcc atcgccaaac ttctcatgaa
    22861 ccaacgcttt cagggttgtg ccgtacacct gcaacatttc atagaaacga tacatcgttg
    22921 ggtcggttgg aatacggtca tcaatgcagc cacgcagtgg aatcatctgc aacagtagaa
    22981 tggcgtcttc gtcgagatcc agcttcgctc cgaccaggcg ggcggcgtcg gcaggaagcg
    23041 cctgctgccc cagcaaagcc gcggttacga aggcttctgc cagaccggta ccgtcggcaa
    23101 tctcggcaaa tgagagatct tttttagctt tgctgagcaa aatggcatcg gcaagatcaa
    23161 gacgaatatt gcggttaatt tgcgactgaa tcatggtgca tctcctgatg gtttaaaaat
    23221 aaggttacgc tgcggtcggt tggcgtagcg gtatggcaca aacgcgcgga ttagctgcca
    23281 gtggcacaaa ctggcgggtt gcgccgtcaa acgctgcgat gctgccgctt tcaatgtcgt
    23341 agacccagcc gtgcagggcg atccgcccct cttcgagcgc caggcgcacc gatggatgag
    23401 tttgcaaatt agccaactga gcaatgacgt tttcacgtac catcgccgca gcttttgacg
    23461 gtaaatcgga atgcgggcgc gcctcattaa cgacgcgggc tgaatcggca taacgcagcc
    23521 agtgggagac ggcaggcata tggtccatgc actgacagct ggcaatggcg gtcatcgcgc
    23581 cacagttgga atgaccgcaa atcacaatgt cagatacccg aagcgcagcg acggcatact
    23641 ccaccgaagc agaaacgcca ccgggttccg gcccgtagga agggacgata ttgcccgcgt
    23701 tgcgaataac gaacagatcg ccaggctcac gttgcgtcac cagctcaggg accagacggc
    23761 tgtcggagca ggagataaaa agtgtgcgcg ggctttgctg tgtcgccagc tgtttaaaca
    23821 aggcttcccg cttcggaaat gcctcgcgct ggaatttaag gaatccatca ataatctctt
    23881 tcaccgttaa cctctgtctg tctctggaat gagaggcaga ctactcgccg attgtcataa
    23941 ggtaaaagtc tcatttatga tgagttctat tggatttact tataggttgg cgaatgctct
    24001 ctcgacatat caattatttt cttgccgtgg ctgaacatgg cagcttcacc cgtgccgcca
    24061 gtgcgttgca cgtctctcag cctgcgcttt cccagcagat tcgccagtta gaggagagtt
    24121 taggcgtgcc gctgtttgac cgtagcgggc gaacgattcg tctcaccgat gcaggagaag
    24181 tctggcgaca gtacgccagc cgggcgttac aggaactggg agcgggtaaa cgggcgattc
    24241 atgatgttgc cgatctgacg cgaggatcgc tgcgtatcgc cgtcaccccc acctttacaa
    24301 gctactttat cggcccttta atggcggatt tctatgcgcg ctaccccggc atcacgctcc
    24361 agctacagga aatgtcgcag gagaaaatcg aagatctgct ttgtcgcgac gagctggacg
    24421 ttgggattgc cttcgcgcct gtgcattcgc cggagctgga ggcaattcct ttactgacag
    24481 aaagtttagc gttagtcgtg gcgcaacatc atccgctggc tgcctgtgaa caggtggcgt
    24541 tgagtcgctt gcatgatgaa aaactggtcc tgctcagcgc ggaatttgcc accagagagc
    24601 aaattgacca ctactgcgag aaagcggggc tacatccaca ggtggtcatt gaggcgaact
    24661 caattagcgc ggttctggag ctgattcgcc gcacttccct ttccacattg ttaccagcag
    24721 cgattgccac acaacatgac gggcttaaag cgatttctct tgccccgcca ctactggaga
    24781 gaacggcggt tttgttgcgg cggaaaaata gctggcagac agccgccgcg aaggcatttt
    24841 tgcacatggc gttggaggaa tgtgcggatg ttggcgagaa tgaatcacgg tagccactga
    24901 cacaggtcag tggctcttca acacagagga aattaccgtt tgtaatcgat ggcttctggc
    24961 tgctccagat atacggtggt ttgtgccggt tgtgtactgg caatcacctt gccgccacgt
    25021 accgaataac gtaccggaac ctgacggcgc agcgcatcaa atccattttc agccggcagg
    25081 ataatcaggt ttgcgctgtt tccggcggca atgccgtaat cctgcaaatt caatgttctg
    25141 gcactgtggt gggtgattaa attcaggcca tcgttaatct gcccgtagcc catcaactgg
    25201 caaacatgca gccccatatg cagcacttgc agcatattcg ccgttcccag cggataccac
    25261 ggatcgaaga catcatcgtg accaaagcag acgttaatgc cggactccag catctcttta
    25321 acgcgcgtga tgccgcgacg ttttggatac gtatcgaaac gtccttgcag atgaatattg
    25381 accagcgggt tggcgacaaa gttaataccg gacattttca gcaagcggaa caggcgtgag
    25441 gtatacgccc cgttatagga gtgcattgcc gtggtgtggc tggcggtgac tcgcgcgccc
    25501 atgccttcac ggtgcgccag ggcagcaacg gtttcgacaa agcgcgactg ctcgtcatcg
    25561 atctcatcac agtgaacgtc gatgagacgg tcgtattttt gcgccagggc gaaggtttta
    25621 tgcagcgact ccacgccgta ttcacgggta aattcaaaat gcggaatcgc ccccactaca
    25681 tctgccccta agcgtaacgc ctcttccagc aacgcttcac cgttgggata cgacaaaatc
    25741 ccttcctgag ggaaggcgac aatttgcaga tcaatccacg gcgcgacttc ctgcttcact
    25801 tccagcattg ctttcagcgc agttagcgtt gcatccgaaa catcgacatg ggtacgcaca
    25861 tgctgaatgc cgttggcaat ctgccatttc agcgtttgcc aggcgcgttg tttcacatcg
    25921 tcatgggtta ataacgcttt gcgctcggcc cagcgttcaa tgccttcaaa cagcgtgccg
    25981 gactgattcc agttcggttg tccggcggtt tgcgtggtgt ccaggtgaat atgcggctcc
    26041 acaaacggcg gtataactaa accttgttcg gcatccaggc tgttttcagt tatgggcatc
    26101 acgccggatt gcgcatcaat ggcgctgatt tttccgtcct gcagatgaat ctgccacagc
    26161 ccctctttgc ctggtaaccg ggcgttaata attgtttgta aagcgttatt cgacactgtt
    26221 aacctccaca tgcgtcattg ctgctgtcgt tttacgattc aaaatcgggt taaggatcag
    26281 atagctcagc gcgccaccta ataccgcgtt gaccggaaca attcccggta accagtggcc
    26341 tgcggcaatc cccagcgcga ccgccagaat cgccacccaa ttgacactca tcatacgcgt
    26401 ggtcgcaaag tgctcatagc ggcgacggtt catcagatag tcggcgatga tcacgccccc
    26461 taccggagga atagctgccg aaaggaaggt caaccagccg acaaaattgt tatacagcca
    26521 taatgcgcag accgtaccga taataccgtt gattaccgaa agggttttgc tcgacatacc
    26581 ggtaatgttg gcgaaaccta aacccgacgc atagagtgcg ttatcgttgg tggtccagat
    26641 attcagcccc agcaccacaa tcgcgggcag cagcagtccc tgagcaatca tcacatcaga
    26701 gatatctgcc atccccagtg ccgcagcgcc cgctgcaccg aaaataaaca tcaacgagtt
    26761 gccgaggaaa aaggccacca tcgccaccag caccgccagt ttggcattgc gaccaaaccg
    26821 gacaaagtca gcggtgagcg tacccgcact gataaatgac cccacaacca gcgccagcgc
    26881 gacattgaaa tctaacggtt gtgcgggaac gaccgctttt aaggcgtcca ggccgcccat
    26941 gccgttaacg gccagccaca cggaataacc gccaaggcag gcaatagccg gaaccgcaat
    27001 caccgaaaga actgtcagcg ccgaaatgcc aaagaagacg gtgacggtca tcagtaaacc
    27061 ggaaacggca atcagcaaat taatatccag cccggttgcc ttgcccaccg gaatagcaaa
    27121 catcgccacg ccaacgccaa accagccaac ctgagttccg cccagtagca gtgaaggcag
    27181 ccatgagcct ttaacaccaa acgagaagcg agcaagaaga tgagtggtca ggccggtttt
    27241 tgcgccaatg taaccgagaa atgaagtgta aataccgagg agaagattac cgatgagaac
    27301 tgcgaggaag aaatcatgat agctaagacc ggttccgaga gtgccgccgg tccacatact
    27361 ggcggaaaag aaggttaatc ccagcatgac gaacgtcaat gccaataccc ctttccgcgc
    27421 cgactgcggg actggcccct ggctaaagtt gttatcttgc gacacgaaat tccccctttt
    27481 tgctgtttga aacccaaaaa atccgccgca ttctattcat ctgaagataa aaagcaatcg
    27541 ttttcgtggg gaaatatatt ttttatatga atgagtgcaa aaaggtgggg gaaaaattat
    27601 cggtgtaaaa acagtgggta ggcataataa gacgcggtaa gcgtcgcatc aggcatcggc
    27661 gcggggtgtc ggatgcgacg cgagcgcctt atccgaccta cggttcaggc ctgtaggcat
    27721 aataagacgc ggtaagcgtc gcatcaggca tcggcgcatg gtgtcggatg cgccacgcga
    27781 cgcatccgac aataccttac tcttccatcg cctggcggat ctgatccaat gacgcaggat
    27841 catcaatggt cgtcagatcc ccaggatcgc gtccttcgca aattgcctgg atcgtgcggc
    27901 gcagcatttt tccggatcgc gttttcggca attgcgagac aaaccagacg tgcgccgggc
    27961 gaccaaagtt gccaatctgg ctgtccacca gcgccataat cgccttctct tgcgaatgcg
    28021 ccacatcacg atcttccaga ctatcgctct ctttcggaat gacaaacgcc accgccacct
    28081 gccctttcag cgcatctttc accccaacca ccgccacttc ggcaacgccc ggatggctgg
    28141 agatactctc ttcaatctcg cgcgtgccca gacgatgccc ggcaacgtta attacatcgt
    28201 cagtgcgccc gagaataaag tgataaccgt cagcgtcacg gatgccccag tcaaaggtgg
    28261 cgtacaccgg gcgggaaaac agcgaccagt aagtcttcac aaagcggtcg tcgtcgcccc
    28321 agatggtctg aatacagccc ggcggcagcg gcccttccac caccagcatc cctttctcgt
    28381 tgacgccaca cggttcgccg gtgacttcat taagcaactg cacgttatag ccatacatcg
    28441 gcacgccggg gcttcccaga cgcgtcggcc tgtcgtccag accgcgagca atcgccataa
    28501 tcggccagcc ggattcggtc tgccagtagt tgtcgatgac cggcacatcc agcgtattgc
    28561 tcacccaact ggcggtcggc tcgtccagcg gttctccagc cagatagagc acttccagcg
    28621 acgagagatc gtgtttgcga atttcagcgg tagggaattt tttcagcacg cgaatggcgg
    28681 tcggcgctga gaacatccgg ctaacctgat atttctcgac gattttccac cacacgccgc
    28741 agtccggcca ggtcggcaat ccttcgtaaa cgatagtcgc catccccgcc agcagcggcg
    28801 cgtaaacgat atacgaatgc cccaccaccc agccgatatc cgatgcgcaa aagaacacgc
    28861 tgcccgcttt gccgccaaaa atggtgtcca tcgaggtcgc cagcgccacc gcatatccgc
    28921 cgacgtcacg ctgcacgcct ttaggtttgc cggtcgtgcc ggaagtgtag agaatgcagg
    28981 aggtttcgtt ggattccagc cacgccaccg gtacccgcgc gccgatgtgt tgatggcgca
    29041 acgacgcgaa atcgacatcc cgcccgctga cgcgcgccat tttcgccagc ccgcgatcca
    29101 ccagcaaaac atggcgtggc tggtgctgcg cctgacttat cgcatcgtcg agcaattttt
    29161 tatagggaat gattttgcca ccgcgcgctc cggcatcagc cgagacaatc agcaccggtt
    29221 tagcgtcatc aattcgcgcc gccacgctgt gcgaggcaaa accaccaaat accaccgaat
    29281 gaatagcacc aatgcgcgcg caggccagca gagtaatatg cgcttcggca atcatcggca
    29341 tatacaccag cacccgatcg ccgcgctgca cgcccagtga gcgcaacatt gaggccaccg
    29401 cgttcacttc gtcatgcagc tggcgaaagg taaaggtgcg ctcttcttct gtttccgaag
    29461 agacggcaat caatgccagt gcctctggct gtttctccag ccagcggtcg atagcgttgt
    29521 ggcacaaatt ggttcggcct tcacaaaacc aacgggcaaa cggcggattg ctgtggtcga
    29581 gcgtttgcgt aaagggcgtc tgccagtcaa tacgccgggc ctgctcggcc cagaactgct
    29641 ccggttcgtt aatcgaacgc tgataaaatt cgctaaaaga cataatgctc tcctgttgaa
    29701 cttacgcctt accgccgttt acttaaatga cgtacaggtc gagatactca ttgaccggca
    29761 tctgttccag gcgagttctg tcgagagaaa cctccagaat gcgctgctgt tggcgagtcg
    29821 ggaactggcg cgcgagattg attttgaatt tatcgaccag tttcggaata ccatcctgac
    29881 ggcggcgagc atgaccaatg gggtactcca ccaccacttc ttcaaatcgt gtgccgtcgg
    29941 tgaactcaag ggtaatggca ttggcgatgg cgcgtttttc cgggtcgtgg tagtcagcgg
    30001 taaatgccgg atcttcaaag caattgatct tctcgcgcag ggcgtcgatg cgtttatctt
    30061 gcgcaacgtt gtcctcgtaa tctgccgccg ttaagcgccc gaataacagc gggatcgcca
    30121 ccatgtactg aatgcagtgg tcgcggtcag ccgggttatt gagcggcccc tttttgtcga
    30181 tgatgcgaat acaggcttcg tgggtgcgaa tggtcacttt ttcgatatcc gccgccgtct
    30241 tgcctgctgc ctgcatctgt tcatagagcg tcatcgccgc ttcaactgcc gtctgggagt
    30301 ggaactccgc cgggaaggag attttgaaca gcacattctc catgacgtaa gaaccgtacg
    30361 gacgctggaa gcggaacgat tcacctttaa aggagacgtc gtagaagccc cataccggcg
    30421 cggtcagggc tgacgggtaa cccatttcgc ccgttttcgc catcagtgcc agacgtaccg
    30481 cgcgggaagt ggcatcgccc gccgcccagg atttacgcgt gccggtgttc ggcgcatggc
    30541 gataggtgcg cagcgactga ccgtccaccc acgccagcga aacggcgttg agaatttcct
    30601 cgcgggtcag gccgagcatt tcggcgacca cggcggtgga agccactttc actaacagaa
    30661 cgtggtcgag gccgacgcgg ttaaaggagt tttccagcgc gatgcagccc tgaatttcat
    30721 gggctttgat cattgcggtc agcacctgtt tcatggtcaa cggcgctttg ccgctggcga
    30781 ccgcgttgcg cgaaagccag tctgccgttg ccagaatgcc gccgaggttg tcggaaggat
    30841 gcccccactc cgccgccagc caggtatcgt tgaaatcgag ccagcggatc atcgcgccaa
    30901 tgttaaatgc cgcctggacg gggtcgagct gaaactgagt tccggggacg cgcacgccgt
    30961 tgggtacgac ggtgccagga acaattggcc ccagcagttt tttacaggcc gggtattcga
    31021 gagcttcaag accgcagccg agcgtgtcga gcaggcagta atgtgcggtg tcgtaggcca
    31081 ctttagagct gatttcgtag ttcatcacgt aatcgacgat atcaacgatt tcacgatcaa
    31141 attccgggcg gatgttgttg atttgagctg acattgggta cgtttcctta ttgttattcg
    31201 tagaggttta ctggcgctta tccagcgcga caaactggcg gtcttccggt ccaacataat
    31261 tggcggaagg acggataatt ttgttgtcct gacgttgttc aatgatgtgc gccgcccagc
    31321 cggtgacacg ggcgatgaca aacagtggtg tgaacatctc ggtgggaacg cccatcatgt
    31381 tgtaggaaac agcagagaac cagtcgagat tggggaacat ctttttgctc tcccacatca
    31441 ccgtttccag gcgatcggcg atgttgtaca tcttcaacga gccgccttcc tgcgagagct
    31501 gcttcgccac acgtttgatc acctggtggc gcgggtcggc gatggtataa accggatgac
    31561 caaaaccaat gaccacttct ttgttttcca cccgcttgcg gatatcggct tcggcttcgt
    31621 ccggcgtttc gtagcgttgc tggatctcca gcgacacttc attcgctccg ccgtgtttcg
    31681 gcccgcgcag tgcgccaatc gcgccaataa tcgcggaata catatcagag ccagtgcccg
    31741 caatcacccg actggtaaag gtggaggcgt taaactcgtg ttcggcgtac agcactagcg
    31801 aaatatgcat cgccttttcc cagctttgcg atggcttttc gccgtgcagc agatgcagga
    31861 agtgaccacc gatggagtcg tcatcggttt ccggctggat gcgttcgccg ttgtgactgt
    31921 agtgatacca ataaaggaga atcgagctaa gtgatgccag tagtttgtcg gcaatatccc
    31981 gcgcgccaga gacggtatgt ccctcttttt ctggcagcgt gcagccgagc gcggaaacgc
    32041 cggtgcgcat aacatccatc gggtgtgacg ccgccggtaa ggcttccagc acggtacgca
    32101 cgttagccgg taaaccgcgc agggctttca gtttcgtttt gtaagcggcg agttcgtcac
    32161 gggtcggcag tttgccgtgg atcagcagat gcgccacttc ttcaaattcg caatgttccg
    32221 ccagatcaag aatatcgtag ccgcggtaat gcaggtcatt gccgctttta cccacggtgc
    32281 agagcgccgt attgcccgcc ggaacgccag aaagtgccac agattttttc ggtttaatga
    32341 catgggtact gttttgcagg atcgttgtgt cgctcatgtt gtcctcgtca tttgtagggt
    32401 cgagatattt ttcgttgtgt tttatttcac ctggctacgg gcaaacaggt cgtcgagctt
    32461 cgcttcgtac tggtagtagt tgatgctttc gtacagctcg ttgcgggttt gcatggtatc
    32521 gatgacgctt ttctgcgtgc cttcctggcg caggacgttg tagacatgct cagcggcgcg
    32581 gttcatggcg cggaacgctg aaagtgggta cagcgccatt gcgacatggg cgctgcgtaa
    32641 ttcgtcggtg gtaaacagcg gtgttgcgcc aaattcggta atgttggcga ggatcggcac
    32701 ctgcaccgca tcggcaaact ggcggtacat ggcgagttcg gtaatcgcct ccgggaacaa
    32761 catctcggca cccgcttcaa cataggcctg tgcacgctcg atcgccgcat ccagcccctc
    32821 taccaccagc gcatcggtgc gcgccatgat cacaaaatca ggatcggttt tcgcatccac
    32881 cgccgcgcgg atccgatcca ccatctcctc tttcgagacg atcgctttgt tcgggcgatg
    32941 accgcagcgt ttcgcaccaa cctgatcttc aatatgcaat cctgccgcac cggctttaat
    33001 catcgatttc accgtacgcg ccacgttaaa ggccgaagaa ccaaaaccga tatccgcatc
    33061 caccagcagt ggcagcgaac aaacgtcggt gatacggcga atatctgtca gcacatcatc
    33121 aagggtagaa ataccgagat cgggcagccc cagcgaccct gccgccacgc caccgccgga
    33181 gagataaatt gcctgatatc cggcacgctg cgccaacagc gcatgattag cgttgatggt
    33241 gccaacaatc tgcaatggat tttctttagt cagtgcagcg cgaaacgctt tacctggaga
    33301 gtgtagagac atagcccatc ctttgttatc aacttgttat tttcgttaat aaaacaaagc
    33361 aaggggtgtg ccacaattgc cttaataaaa ttaaatgttt aatttcaatt tgttagagat
    33421 aacgtcatga actaatccgc aaatatgcgt ttcagttaac gtttcaggca atgtttcacc
    33481 gcgtttcatt gcaacaatta tgaaacaaga ctaaacccaa tattcggttt cttaactttg
    33541 cggtgcgcta tggcacatcc accacggctt aatgacgaca aaccggttat ctggacggta
    33601 tctgtaacgc gcctgttcga gctgtttcgc gatatcagcc tcgagtttga tcacctggcg
    33661 aacattaccc ctattcagct tggctttgaa aaagcagtga cctacatccg caagaaactg
    33721 gcaaacgaac gctgtgacgc catcatcgct gctggctcta acggcgcgta cctgaaaagc
    33781 cgcctgtcag tgccagttat tttgattaaa ccgagcggct acgatgtgtt acaggcactg
    33841 gcaaaagccg gaaaactcac ctcttctatc ggcgttgtca cctatcagga aaccattcca
    33901 gcgctggtgg cgtttcaaaa aacctttaat ctgcgcctcg atcaacgtag ctacattacc
    33961 gaagaagacg cccgcgggca gattaacgag ctgaaagcta acggcactga agcggtggtc
    34021 ggcgcggggc tgattaccga tctggcagaa gaagccggaa tgaccggaat ttttatctat
    34081 tccgccgcca ccgtgcgcca ggcgttcagc gatgcgctgg atatgacgcg catgtcgtta
    34141 cgccataaca ctcacgatgc cacccgcaac gccctgagaa ctcgttacgt gctgggcgat
    34201 atgctcggtc aatcaccaca gatggaacaa gtacggcaga ctattttgct gtatgcccgc
    34261 tccagtgcag cggtgttgat tgagggggaa acggggacgg gcaaagagct ggcggcccag
    34321 gcgattcatc gggaatattt tgcccgccac gatgcgcgac agggcaaaaa gtcgcatccg
    34381 tttgttgccg tcaactgcgg ggcgatagcc gaatcgctgc tggaagcgga gctgtttggc
    34441 tatgaggaag gggcgtttac cggctcgcga cgcggaggtc gcgccgggct gttcgaaatt
    34501 gcccacggcg gtacgctgtt tctggatgag attggcgaaa tgccgctacc tttgcagact
    34561 cgcctgttac gggtgctgga agaaaaagag gtcacccgcg tcggcgggca tcagcctgtt
    34621 ccggtagatg tacgggtcat tagcgccact cactgcaagc tggaagaaga tatgcagcaa
    34681 ggacgttttc gccgtgatct gttttatcgg ctgagtattt tgcgtctgca attgccacca
    34741 ctgcgcgagc gggtggcgga tattcttccg ctggcggaaa gctttttgaa agtgtctctg
    34801 gcggcgctct ccgccccgtt ttctgccgca ttacgccagg gattacaggc aagcgaaacc
    34861 gtgctggtgc actacgactg gccaggtaat attcgtgaac tgcgcaatat gatggagcga
    34921 ctggcgctat ttttaagtgt ggaaccgacg ccggatttaa cgccgcaatt tttgcagctg
    34981 ctactgccgg aactggcgcg cgagtcggcg aaaactcccg ctccacgctt actgacacca
    35041 caacaggcac tggagaaatt taatggcgat aaaacagcag cggcgaatta tttaggcatc
    35101 agccggacga cgttctggcg gcggctgaaa agctgaactg ccgaatgcag cgcgaacgct
    35161 ttatccggca taaatttcaa tagatgatca ccgataaact tacgcatcgg gcatgtttac
    35221 atagggttca gaattacttc ttcttataaa tatttgccgt gccgtggatc ttattgtcag
    35281 tttgaccgga ggtcagtacc aacacatcag cccctttttc atccgctttt ttgatcaaat
    35341 cttcttttgc atctgcagtc gacatttcat tgctggttga aatatcacct attttttcat
    35401 actgcgattc aactttttca aattccgctt tggtcatcaa ttcagcggca tagacattgg
    35461 taacggctaa cgctaacgca ccgactaaca ttttagagat tattttcata ttacggctcc
    35521 agagttgtta tcacactatc atccacccac attattgggt tgattaataa ttagttcagt
    35581 cagggcataa acgccagtaa ccggaaataa gacactttgt gatctctctc gcacccgatt
    35641 atttttggta gaaatatgat gaagttactt cacttattta tggatgaaat cactatggat
    35701 cctttgcacg ccgtttacct gaccgtagga ctgttcgtga ttactttttt taatccggga
    35761 gccaatctct ttgtggtagt acaaaccagc ctggcttccg gtcgacgcgc aggggtgctg
    35821 accgggctgg gcgtggcgct gggcaatgca ttttattccg ggttgggttt gtttggtctt
    35881 gcaacgctaa ttacgcagtg tgaggagatt ttttcgctta tcagaatcgt cggcggcgct
    35941 tatctcttat ggtttgcgtg gtgcagcatg cgccgccagt caacaccgca aatgagcaca
    36001 ctacaacaac cgattagcgc cccctggtat gtcttttttc gccgcggatt aattaccgat
    36061 ctctctaacc cgcaaaccgt tttatttttt atcagtattt tctcagtaac attaaatgcc
    36121 gaaacaccaa catgggcacg tttaatggcc tgggcgggga ttgtgctcgc atcaattatc
    36181 tggcgagttt ttcttagtca ggcgttttct ttgcccgctg tgcgtcgtgc ttatgggcgt
    36241 atgcaacgcg ttgccagtcg ggttattggt gcaattattg gtgtattcgc gctacgcctg
    36301 atttacgaag gggtgacgca gcggtgagca gataatttac tctctacgta atcaacctga
    36361 tttgccgaat cccgaccatc ggcgatgata gcccccgtaa tgcgtggtcc ttatttccga
    36421 taagtttact tgagtcttta gcttcccatt tcagcccact ctcccctgtg tgggcttttt
    36481 tcattggtct gacccgtcct gaatagccaa acaatggcaa tgttccactt tgcagggatg
    36541 ataataaggg gatctgattc agaagagttt tgaaaagttg gaccgccatc gaggcctcga
    36601 accccgtacc atacaacaat cctggttgtc gtctgctctt cctgatgagc taatggcggt
    36661 ttttgatatg agccttacag catattttct gtcaggcttg agtgcgatgc gtaaggtaca
    36721 gaaaagaccc catacaaaca tgatggaatt atgatttttt tcatatgaat gtcaacccac
    36781 acattcacaa aacatctgca tgagatcatt ttttatacac cacatagctg cgaaaccact
    36841 gctggtgctt ttttaagcga agcgtcgctg atcacaacaa aatatcggcg aaaaggcggt
    36901 ggaatgccat ttagcatacg gatatacaga tgaaatgatt acctgctttg cgattcactc
    36961 tctggtggaa taacaacttg caggtggaac cctttctgaa ctaaagagtg tagcggattt
    37021 taacgtgctt atttacagga taatatctgt cacctaattc ctgcactcta ttcgatatcc
    37081 attcatttaa tcgtgcgtca tctctgtatg tttttacgtc cagaacagag agcaataaca
    37141 tcaccagtga ttcaggaaaa acagggtaga tattttttaa gtgtaataat aatccatgcg
    37201 caagcgcctg cttaatgggc gcgtctgtct ccggaataga cgacatttcg cgaagccatt
    37261 tttccacatg catacactct ttccctttag ggaaaagtct cagcaaaggc atatggtgtt
    37321 caagaagaat acatttgcaa tctgttcgca ctgaatcgat aaactgaact aatttcttaa
    37381 gcccggtaaa gcgtccagcc tgcacatttg ctttaatatc aagaaacgca tcatcctggt
    37441 catataccca aactgtccct ttttgatttt caagtatgcg aaaaacaaac ttaaccgggt
    37501 gatcagggta atccatccag tcataaaatc cagggaaatc attaaactgc acatttatgg
    37561 tgagtgcgtt atcgtctgac cactcacagt ctcgtacggc agccatgaga tgaatgagtt
    37621 tgtcgtcatc gactaaatca tcacgacaat cataaggata atcataatat tgtttaatca
    37681 aaaccttgaa tatatcgaca caggaaacca gggagctttt gatatcctct ggtaactgat
    37741 tgtttttaac cgtattaagt atgctttgtt gcgtatatgg catcaaatta ttgtgcggag
    37801 cttttaatga tatcatcgcg ctctctctta aataaaaata aacgtcgcag ataataaagc
    37861 tctgattaaa ccaggcacta tcagaaatcg ctcataacta acgtgtgaag tattgtgtac
    37921 tggagggagt taatttaaca atttgaattt acgttatagg ttttactgct actttgatat
    37981 tatttcgctc tccttaacta aaaagttaag tggagagcga agttaatatg ttaaaccaca
    38041 gcgtatttat taattttttc agtctgttag tgtgcgatta tcgataacaa aacgatattt
    38101 cacatcacca cgcagcattc gctcataggc ttcattaatt tgatcggccc gaatcatctc
    38161 tatatcagcc acgatgccat gttcggcgca aaaatcgagc atctcctgag tttctggaat
    38221 gccgccaatc atagaaccgg ctatcgcacg gcgtttcatg atcaggttga aaacttccgg
    38281 cgatttatgc ggtgtcgcag gcgcaccaac cagcgtcatg gtgccatcac gcttcagcaa
    38341 ggtggtaaaa tcgtcgagat tatgtggcgc agctactgta ttcaaaatga aatcgaaact
    38401 cttcagatga gccgccatct catcggcatt gcgtgagtta acaacttcat cggcccccag
    38461 ggcttttgcc gcttcgcgtt ttgcctcaga agtggtaaat gccaccacat gcgcccccat
    38521 cgcgtgggcc agcttgatcc ccatatgtcc cagaccgccg atgccgacca cgcccacttt
    38581 tttacccggc ccggcctgcc agtgacgtag cggcgaatac gtggtgatcc ctgcacacaa
    38641 caaaggagcc accgccgcca gctgctcttg cgggtgacga atacgcagaa catatcgctc
    38701 atgaacgacg atctgttgtg agtagccgcc cagagtatgg cccggttcgt ccggcgtcgg
    38761 cgagttatag gtgccggtca tgtgatcaca gtagttttcc agcccgtctt cacactcttc
    38821 gcaatgttta caactgtcga caatgcagcc gacaccgacc agatcgcccg gcgcatgttt
    38881 ttctacctga tcaccaacgg ctaccacacg ccccacaatt tcatgacccg gtacgcaggg
    38941 gtaaaccgtc cccgcccact cggaacggac ctggtggaga tcggaatggc agacgccaca
    39001 gtaagcgatt tcgattttga catcattcgg tcccggttca cgccgggtga tatccatcgg
    39061 ttcaagtggt tgtttagcgg aatatgcacc aacagctttg atcttcattg tgtttactcc
    39121 tgattagcta tgtgtatttg gcgatgcctg atatgtcatc gcggcaaaac gcgtccattg
    39181 aatatagcca atatttaaac cacccgatcc agccccaaca tgccgcgagc ctgaatgaaa
    39241 caaagcagca acgttgccca taaggcgagc acctgtaagt gtgatatttg ctatccgtaa
    39301 aagactaaaa tcggcaaaac tgatgatctt ttcacgtaga caaatagtta tctacacaac
    39361 gtcaataagg gcatattcct caaagataat atccatttct gccgacacac tcatattcag
    39421 gttgccccac tccctggggc ttaaatctct tactattcac cgttgcaggc gtttcaaatg
    39481 cgcatcaacc tgccacactc ccccacacca gttgcccttt atggaacgtt gcggttctcg
    39541 gcgagatacg cgccaccgcc tcggcggaac aggaggcgtc caccagcaca aaactggcgt
    39601 catcctgcgc tttgggccac acgcgctcgc ctttttcgtt gagcggcaat acatcgccag
    39661 tggctaaaaa cagcgagcgg gagaggttct gttcgttagg acgaatatag agctgcgcgt
    39721 acagattggc tttttccagc atgtcgccca ggccgtaagg cgaccagtgg tcgataacgc
    39781 tgtcagtgcc agtcatcact tttacgcctt tgtcgtgcaa ctgtttgagc ggcatatgca
    39841 gcgtgccaat cggcaccgtc gaggcgatag aaatttgctg cgccgccatc cggtgcgcca
    39901 gttcatctac ctgttgttcg ttgagcgttg ccagcgcaaa ggcgtgactg atggtcagct
    39961 tgcccttcag ctgtggcgtt ttctctaccg tttcaaccat ataattgatg gctgccacgc
    40021 ctgccggagt ggtttcatgc aggtgaatat cgacgccttt gtcgtagtcc agcgcaatct
    40081 gaaacatggt gtcgagggat ttttccatcg cgccatcaac actggtcggg tccagcccgc
    40141 cgacgtaatg cgcacccgcc tgcatcgctt cgcgcattaa gggttccgat ttcgacagca
    40201 gcaaaccgtg ctgcgggaag gcgacgattt cacactcgaa gcccgcctga cgccgcgcca
    40261 gcaccgcctg caaattttgc agatttttta ggccggaaac cggttcgata ttgcagtggc
    40321 tgcgggcaat ggtggtgcct ttcgactgca atagatcgat cagtttttcc gcccgctcct
    40381 gcgtatacgg ttgtaactcc ggcagcattt tctgctcgag tttgatcatg tcctggatag
    40441 tggtgcctgc cggacgattg agcgagcgcc acggcccgcc gtagaaggtt ttgtcgagat
    40501 gaatatgcat gtcgcgagtg gtgggtagca tcaacttacc gccagcgtca tagtgcggca
    40561 gcgtggcgtc cggatgctgc ttgttctcgc gcagggcgac aattttgccg tcctgaatct
    40621 ccacggtctg gcgcgccgtg cgggtctgca ccgccacacc gttttcgtag tcaaaaccgg
    40681 tttccagcag cacgttatcg agatagtaat gcgggtcagt gattttcatg gtgttgttcg
    40741 cttcgcagtt tagggtgcca gctaccgccg catgggcgag cggcacagag gtaccaaaca
    40801 gcgcggcggc ggtgaccatc ttaccgctct ggctcagaaa ttcacggcgg ctattgcttt
    40861 ctttcatcct acgtccttac gttttaataa tatgggtgcc cgtttcaccg cgcagcgccg
    40921 caggcaggca ttccggcgtg gtgataatca cttctttgcc gccctgctcc aggaacgtca
    40981 ggctggcgat gatttttggc aacatgctgc cgggcggaaa atgcccttcc tgcatatagc
    41041 gagtcatggt ggcaatatcc acccgatcca gcgcctgctg ctgcggttta ccaaagtgga
    41101 tacagacttt ttcgacgccg gtggtgatca ccagaatgtc ggcgtgaatt tcacgggcca
    41161 gcagcgcggt agagagatct ttgtcaataa ccgcgtccac gctttggtaa tcccccgctt
    41221 cagtacgcac taccggaatt ccaccgccgc ccgcgccaat tacgacaaaa ccttgttgga
    41281 tcagcgcctt aatggcgggt gcttcgacaa tacgtttcgg ttccggcgag gcgaccacgc
    41341 ggcgatagcc ccgtccggca tcttcaacaa aacaccagtc agggtttgcc ttttgtagtt
    41401 tgtcacgctg gctttcactg aagaatgcac caatgggctt ggtgggatgg gcaaaacctg
    41461 gatcgttttt atccacttcc acctgagtta ccacggtgac ggcttttttc tcgccgtgac
    41521 gcgccagccg gttattcagc gcctgttgga tcagatagcc gatgccgccc tgcgtatctg
    41581 ccacacagtt cgccagcggc gttaagggca gcccttcgcg ctcgtgggca atctccgcac
    41641 ggcgtaaatc cagcccgacc tgcggcccgt taccgtgggt cagcacaatg tcgtaatcgg
    41701 aagccagcat ttccagcacc gtatcggcca cggctttcac cgcctccgcc tgatgctcaa
    41761 tcgactggct ggcgttatct ttgataatgc tgttgccacc aatggcaacg accacaagct
    41821 ctttcatgtt gttatgtccg ctacagaggg tgattaggtt atgaccaacg ccagactgcc
    41881 tgatgcgcta cgcttatcag gcctacaatt ctcgggccat ccgtaggccg gataaggcgt
    41941 tcacaccgca tccggcataa acatgtaagt cagacgccgt aacgttcgca ccagccgaga
    42001 atggctttct cgaagcaggc gaatggtgga tgcacaatgc ccgcgccaat catgccgacg
    42061 cccgcatctt tatgggcgat ggcggtgttg atcaccggca gaatgccgct gctacccacg
    42121 cgggtgatgt cgattgccga cggcacgccc ataaaaccga gcagcggaat ggtgacgttg
    42181 gggttttcgc caagggtgat ttcgcgcatc tgacgggaga aatcgatagc ctcttccacc
    42241 gtgccgccca ccagtgcgac gattgcaggc gcggtcgcca tggcaaaccc gccaataccg
    42301 taggtttctg tgatggcgct gtcgccgata tccagccccg aatcttccgg cttataacca
    42361 gcaaacatcg ggccgatgac ctgctgcgct gggccggtaa accactgccc cggcaggccg
    42421 ctgacccgca ggccgaactc gacgccgtta cgcgccatgg tggtgaccac ggtgctgtat
    42481 tcgatgccgt gcgccgcatc catcgccgct ttacacatcg ccatccacgt cgggccggag
    42541 aagtagtcgc tgctggcgac aaactcgaac acttcgcgct gttgctcgac ggaatagcct
    42601 gcctgaatga tccccggcgt cagtgcctga atcagcagcg tcgtcccggc gttattgcgg
    42661 ttatggcact catcgcccat atgcagcgcc tgcgccagca ttaagcgcag atcgatctca
    42721 ccgataattt tcatcgcgtc gcgcagcatt ggcccctgca catcgcgcat ccagttgagg
    42781 cggtcgatca cgctctgatc gttggcaccc atacgcaaaa tcttcgccat ctgctcgctc
    42841 atattggtat aagcgatgtt gccgtaggtt ttgtttttca cgatgtgcat aaacatcgag
    42901 gccgaggtaa cgcccgccat cgatcccacg cagtcgtgtt cgtggcacgg cgagaaggtg
    42961 atctccccgg aggccgccag ttcagtcgcc tcatcgagat ctttcgccag cccttcgaac
    43021 accaacgccc cggtgaccgc gcctttcatc gcgccgcaca ttttttccca ggtgatcggc
    43081 ggcccggcgt gaagaatggt tttcggcgtc atgcccggca ccacgttaat cgcctgatca
    43141 aaaccaatca gcaccggatg cgactggata atgcgctcca gcgcttgctg gttagcggcg
    43201 gcgattttgt ctgccagcgg cgaatcggca atgttatcca gcgcctgcac cacctgcata
    43261 ttgccctgcc ccggcggcgt ccagtcgagt tgagtcactt ctacatgctg ctttttcagg
    43321 tcatcgctaa acatggcgat accgacgtta ataacgttca acggttggct aaacagtgac
    43381 tggctcatca ggcttcctcc cctttgcaga taaattcacg cgccagcaat ccggtattgg
    43441 tgctgctgct cgccagaatc actccggcat caagcagcat ctggctttgt tgttctaatg
    43501 atggcgtatc gagatcggtg cccagcacat aggcgagaat gatcaactcg cgtccttcgg
    43561 cggcggcgat cgctttcgct tctttgatcg cctcgatggt ggagccgacc ggatcttcat
    43621 gcgatccaaa tccgagcaca aaatccatca cgatcaccgc cacttctgga tcgcgcgctt
    43681 cttcgatcaa gcgactgatg cggttggtgg ggtcaatcat tggatgcggc ttgccgttgg
    43741 tgaagtcgtc atcgccaaag tcgaggaagg tgtgtttgat gctgcggttg atatcttgca
    43801 ggcggaattc aggatcgggc tgaatgttgc tgtagacatc gccatgtttt tccatcaccg
    43861 cgaacatggt ttcgtcgcac agcgtgccgc cgcagaacag gccacgtatg tatttctgct
    43921 gcggttgcag acgcgcacgc acatcagcaa tcaacggctg gttaagcgta tgcaggtcga
    43981 gattttcctg tttcacgccg gagagcatca ccgcttttag cgccgcctct ttgctgccgc
    44041 gggcaaactg tagcccctgc tcatccaccg gcgtttcaac gcgaccgagg aagcagacca
    44101 ccaccggctt gcggcaggcg cgcgcgcgtt ccagcacttt acgggccacc gcaggcgcgg
    44161 gcggtttgga gataagcaca atgatttcag tttgcggatc gttttccagc atcccgatgg
    44221 cgtcgagcat catcaggccg ccgattttct cgctcaggtc gcgcccgccg gtgccaatca
    44281 gttgtgaaac gccgccacca aattcatgaa tgcggacgct cagctcctgg ctgccggtgc
    44341 cggatgcgcc aacaataccg atgttaccgc gacgcacggc gttaccaaag cagagcgccg
    44401 cgccgttgat aatcgccgtg ccgcagtccg gccccatcat cagcagccct ttttcatggg
    44461 ccaattgttt gagcgccagt tcatcttcaa ccgagacgtt atcggaaaac agcatcacgt
    44521 tgagatcgtt ttgcagcgcc tgacgcgctt cgcgagcggc aaacagaccg ttgaccgaaa
    44581 tcaccgccag gttactttcc gggatatgct ttttggcgct ggcaatagtg gcgtaacgcg
    44641 cctcgtgcga gccgctttgc gctttggtgt tgaacagttc ttcaatctcc accagcaact
    44701 gctcgttgtc cgcacccgat ttaccattga tgacaatcat caggtcgccg tttttcgcct
    44761 gttccagctc cggcgtcagc agtcctaaat ttttcagcac acctttgttc atttcggtcg
    44821 ccatcgccac aaatgcctgc tcgacgccgt cgagtttatt tgcacgcgtg gagatagaca
    44881 tcagcgagac agaatcaaaa taggtattcg gtttaatgac tattttaact gacataattc
    44941 cctccgtagg tttggctcag cgcacaacca tcggccatgc catacagcat gtcgcagccg
    45001 gaactggagc caatatgttt aattttttct attgcctgag ttgcgttccc agggatgtca
    45061 taaataatgt tgtgaataaa actatgaata ttttcccggc aacgttgttg taatgcggct
    45121 tccagcgtta tagcacttaa taatgtggta ttatttctgc cgcgttgcag accgagataa
    45181 aattcctctt tgtatttttc cgccggatgc ccgggaataa ataaaacaag cgccagacct
    45241 gttaaataat cgtcggctga tggcgtaagg ccaatcccta accccatcat actggcgacc
    45301 gctgaggcga tattttgttt atcgtttacg gcctgaataa ctgcccgtcg tttaatatgt
    45361 aattctttat ttaacgcctg ataaaacgga ttatcgccgc gataattaaa cagggtttca
    45421 ttttccccca ggtgctgatg aataatgtcg tgccactgtt gccaggcgat aaggtgaaaa
    45481 ttcacagcgg tcatttgcaa ggttggggac agccaacgcc gacaacgact catctctata
    45541 tgaagatgtt gaccaaccgt aatgccttgc tcgtcaaact gaaccttatc accatgccgg
    45601 aacagaccat taaagtgagt gagtgccaac cgacaactgt ttggcgcatt gtcgtactct
    45661 tcacaaagca gcgtcagcaa ctgctggcga gccgggagtg cgatatttac cgctctggca
    45721 aagacctgtt caacctgtcc ttgcccccgc tgcgccagaa aatccgcatc cgcagttagc
    45781 gcccacacac accgtctttc tggtgtgttg atagcaagcc tcccggttta tgcacctgcg
    45841 gcaatcagta actgcgcaat ctcttcaaag ccccgttccc gcgccagttc cagtggcgtt
    45901 ttgccatatt tatcggtcag atgcgggctg gcaccgtgtt ccagcaataa ctggacaatc
    45961 gcctgctgtt taatcccgcc atcattaagc acaatcgctt ccagcagcgg tgtccagcca
    46021 acatggttgg tctggttaac gttaatttcc gtatgcgcca aaagctcttt tacaatactt
    46081 aaatggcctt tttcacaggc aggcgtcagg ccgacaccgc caaaacgggt gacgcaatta
    46141 aggtctggtt tagccggtaa aataattcgt agcagcgtta aatcaccgtt cagacagctc
    46201 attaaaaaag gatttaaaca tgtcaggtct tgtttattaa tatccgctcc ggcatcaatt
    46261 aatgcctgaa cgcaagcata ttgctgatat aaacttgcca gcgtaattgc cgttttcccc
    46321 tggcgatcgc aggtattaat atcgacaccc agcgcaagac aggtttttac tttatcgata
    46381 tcgccctgtt gtgcagccaa taaatagtcg gcaggtagat tttttatcgt cattcacatt
    46441 cccctggttt ttgtggctgt tttcgatgaa ttgagaatag caacgataat ctgataaaag
    46501 aacccgctta aatttaattc gccgtatttc gcctttttgt tgaaagatgt tcaacaatct
    46561 tttcgcagtg tgcggtagtg caatatttgt ttctttgacc cgataccctt tcactcaatc
    46621 aggttttatt tctctatagt ctgcacatgt aagagtagct ttcgtccatg tctaaccaga
    46681 ggggaacaag atgaacggac tcacggcaac gggtgtcacc gtcggtattt gtgcagggct
    46741 ttggcagtta gtgtcttcac atgtcgggct ctctcaggga tgggaattat taggaaccat
    46801 tggcttcgtc gccttttgca gtttttatgc cgcaggcggc ggtaaatcgg ggtttatcag
    46861 aagtctggcg gttaattctt ccgggatggt gtgggcattt ttcgccgcgc tggctgccgg
    46921 ttggctggcc tcggtgagtg ggatttctgg gttctgggcg agcgtgatta ctaccgtgcc
    46981 tttctcagcg gtagtggtct ggcaagggcg tttctggtta ctgtcattta ttcccggtgg
    47041 ctttctcggc atgacgctgt tttttgccag cgggatgaac tggacggtga cgttactggg
    47101 ttttctggcg ggtaactgcg tggggataat ttcagaatat ggtggacaaa aattaagcga
    47161 agcgacgact aaacgcgacg ggtattaaaa gttattcaaa aatgattatc tatctttaac
    47221 cgatacctct acagtcatag ctggagaaat tcatagtaga taatggcgag aatggtttaa
    47281 tgaactcaat ttttaccgaa gaaaacctgt tggcatttac taccgctgcg cgttttggca
    47341 gcttcagtaa agccgcagaa gagttgggtt taaccacttc cgccattagc tacaccatta
    47401 agcgtatgga gacggggctg gatgtggtgc tgttcactcg cagtacccgc agcattgagt
    47461 taacggagtc cgggcgttat tttttccgca aagctaccga cctgctgaat gatttttatg
    47521 ccatcaaacg tagcatagat actatttcgc agggcataga agcgcgtgtg cgcatttgta
    47581 ttaatcagct gctttatacg cccaaacaca ccgcgcgctt attacaggtg ctgaaaaagc
    47641 agtttcctac ctgccagatt accgtgacga ccgaagtgta taacggcgtc tgggatgcga
    47701 ttattaataa tcaggccaat atcgccatcg gcgcacccga tacactactg gacggcggcg
    47761 gtattgatta cactgaaatt ggcgcgattc gctgggcttt tgctatcgcc ccggatcacc
    47821 cgttagcctt tgtgccggaa cccatcgccg aaagccaact gcgtctctac cctaatatta
    47881 tggtggagga caccgcacat acgattaata aaaaagtggg ctggttgctg catgggcagg
    47941 agtcaattct ggtgccagat ttcaacacca aatgtcagtg ccagatttta ggtgaaggaa
    48001 ttggtttttt gccggattac atggtccgtg aggcgatggc gcaatccttg ctggttaccc
    48061 gacaaatcca taacccgcgc caggattcac gcatgttact cgcaacccag cactctgcca
    48121 ccggtcaggt gacgcagtgg attaaaaagc aatttgcgcc aaatggcata ttaacgggga
    48181 tctatcagga tttactgcat cgggagaatt aatcgctgcc aacctcccga tgcgctgtga
    48241 atagcggttt acatcaacca ccagctttca ttacccattc agaaacaaat ttattacccg
    48301 gtactggcgg ggagtacaaa tagccctgca aaaagtgaac gcctttaccg atcattaaat
    48361 ccgcctgctc ctgggtttct accccttccg ctacgatact cagaccaggc ttacgcgcca
    48421 gttcgacaat attgtccaca atatgaccgg agatttcgtc gacactcgcc atttgcacaa
    48481 atgacttatc gatcttaata aaatcgaccg ggaacgcctg caagtaacga taggtcgcat
    48541 aacccgtacc aaagtcatcc agcgcaaagg taatgttgtg ctgatgaagg ctgtcaaata
    48601 tcgctctggc ttctggcgtt accggaatag ggttacgttc cgttagctcg agaaccagtt
    48661 tgattttatc gtttcctaat ttcttgacca ggttcaggca ttctttttca aatcccgcgg
    48721 ccaaaaaaca acccgccgag acgttgatac caatatggaa attatccggc agcaaatgtt
    48781 ttaccggcat cagaatatcc gcagtctgtt tcatcagttg gcgggtcatt atgacgataa
    48841 gaccagatga ctccgccaga ggaataaact gatccggtgg gataattccc gtttgtggat
    48901 gttcccagcg gacaagcacc tcacagcccg tcagtacccc agtctgcgcg cagaaaaccg
    48961 gttggatcca cggtttgaat tcgtggtttt ccaacgccag actgatggct tcaggcgtga
    49021 cgatatggtg atagtgatta tcattaatat atctgtgact attactcccc gtggcggata
    49081 tgatctccgg attgtactga aagaaaatat cgcgccagaa agtaatatcg ctctgaatcc
    49141 ccagtttctc aaagagctgt ttcttttgat gtgaaattgt ctttgcactg cgatttctgt
    49201 actgtgatat ttctgttaca gacattccct gcatcagcag cttgagtagc agtcgttctt
    49261 tctcggatac gctaccaggc aatgacaaat gaaccgttgt accgaactct tgcagaaaaa
    49321 cacgaaaatc acatgaattc atgaacacac ctttatcttt tatcgctgtt cagtatgagt
    49381 gattacctgt cggttcctga gcacacataa ggggtaacca taaacatcag cgatgccata
    49441 cgtataacat cccacatcac tatcaagaag attatcttta cctctgatgc tcgctcaaaa
    49501 acagttacgc taaatacctt aaaaaggtaa tcacaagtca aactcaggag ataatcacgc
    49561 aagaatgcaa tattaaaatt cagatactca tttttggtaa taaagaagta gttggactat
    49621 ttcaatattt ataatttccc actacatact ggcatccatt catgagcaaa ggcgcattat
    49681 atgaatttaa caatccagat caactgaaaa tacctctccc tcataaacac atagcgtcaa
    49741 cattcaatga cataatgagt aaagatgttg gttatacata cgtatcatta ctctatgcct
    49801 gtcccttaaa aacccactca ttaagactga atccattcag caaatgataa ccaatattat
    49861 agcaatgcca ataaaaacaa tatttaaaac attcaataaa atatcacaca cagataatta
    49921 aaccttaaat aacaaatggt tgcgttaatt tcagttgatt tacgatacac atttctcatg
    49981 aataatgcgt catttcaaat gatcagaaat atttacatta aataagacta aacttattga
    50041 acattcgcaa cagcccaaat gtaggttttg ggctgcaata gttcttttct tttttattat
    50101 ctgttgtctg gtaatcatca atagatacta agggcttaac cgtatagata taacgtatca
    50161 agaggtagga gaaacagcgc catatgcaga catggcgcgg ttttatgcaa taacaatcac
    50221 gcgtccggga acatcacgct atggcccggc gcttcacgat ggagatgaat aaagttaagg
    50281 tgccgctcgt actggtcaag aatatcggtg atcacctgct ctttgctgta gtccatcagg
    50341 tcgttgccct ggctgccttc taacaggaag gtttccagcc ggtagtaggt cgatttaccg
    50401 ctgcgtgcgc ggtaggtaaa gcccggcacc gaatattgct gcggccaaat ctgatagaca
    50461 aagttttgct cttcgcccat atgcaccaac aaatccagat gccccaactg ttgtccctct
    50521 tccggcggca ggctttttag ctccacgtac gcgccgcgca accgcagctc ctgcgccact
    50581 tcttccattg ccgggtaaca gaccgtctcc atcatctgtt tagtgtaacg cgtgcccgga
    50641 taattcatca ggcgcgagag acgttttttc cagctcaggc gatcctgaag ccccagcggt
    50701 cgcggcgcgg tatcgcggtt ggcactttca cggcggtaat cttctacctt cagagattta
    50761 tacaaccccg ccatcacgaa gaaaatcaca aagctgaacg gcagccccat gattaccgtg
    50821 gtgttttgca gcgcggatat cccgttggtc atcagcatgc cgagcgtcag cagaccaatc
    50881 gccaccgacc agaagacgcg cagccagccg ggggcgtcgc tgttgatatc tttaagctgc
    50941 gaggtgaaat tccccagcac cagcgcaccg gagtccgccg aggtcacata aaacagcagg
    51001 ccagtaatgg tggcgacgga ggcgctaaag gtaaacgccg gatactgcgc cagcaggctg
    51061 tagaagccgc gctccggatg gaccatcgct tcctcggcaa atgccgcgcc gccgtggata
    51121 atttcataca gcgcgctatt gccgaacacc gagagccata acagcgtgaa ggtaaacgga
    51181 ataatcaacg tgcccagcac gaactggcga atggtacgcc cacgcgagat acgcgcaagg
    51241 aacaagccga caaacggcga ccatgccacc caccatgccc agaagaagag cgtccagtta
    51301 ttcatccact caactggacg gtcgaaggca aaactgttga gcgtcatgcc cataaagcga
    51361 ttcacatagt cgccaacatt cagcaccagc gcattaagca ggaacgaagt gtcgcccata
    51421 aacaatacga acaggatcaa tcccagcgcc agcgcgacat taagctccga taacacgcga
    51481 atgcccttat cgacaccgga ggtgacagag atcgtggcga ttatcaccga caaggcgatc
    51541 agtgccgctt ttgccgccat cgaatcggga atatcaaaca gtacgctcaa gccatagtta
    51601 agctgcacca caccgatacc gagcgtagtg gcaataccga agatagtgcc gatcaccgct
    51661 gcaatatcca ctgagtgacc tataggtccg ttaatccgtt taccgaagat cgggtacagc
    51721 gccgagcgga tggtgagcgg caaattataa cgatagctaa agtatccgag cgccatgccc
    51781 atcagcgcat acatcgacca gccggttaag ccgtagtgaa acagcgtcca gaccatcgcc
    51841 tgacgcgcgg cctcaatcgt ctgtcccgcg ccttccggcg gctgcatata ctgcgttacc
    51901 ggttcggcta cggaaaagaa catcaggtcg ataccgatcc cggcagcaaa cagcatcgcc
    51961 gcccaactca gcaggctgaa ttccggtttg gattgttctg gcccgagctt caccgaacca
    52021 aaacgcgaac aagcgataca gaccacaaag acaatataga gcgttgccgc cagcagatag
    52081 taccaaccga aggttttaga cacccagtcc agcgtgcggc caatccacag ggccgagaag
    52141 tcgcgaaaca ggatcgttgt cagggaaaac aacaaaatca gtccggcgga ggtgtaaaac
    52201 accaccggat tgattttgtc cttttccctg ctgtgtgaaa ggtctgtcat ccagtatccc
    52261 cactgttatt gttaccattt aaaatcaaat tcgtaacaat taagacacat tttatattga
    52321 acgtccaatc aataaccgct ttaatagata aacaacgctg atgaatggag tggcgaaaat
    52381 gcccaaattg gggatgcagt cgatccggcg cagacaactg atcgacgcca cactggaagc
    52441 aataaatgaa gtgggcatgc acgatgcaac gatcgcgcag atcgcccgcc gtgcaggcgt
    52501 ttctacgggg atcatcagcc actatttcag ggacaaaaat ggtctgctgg aagcaaccat
    52561 gcgcgacatc accagtcagt tacgtgacgc ggttttgaat cgattacatg cacttccgca
    52621 gggcagtgca gagcagcgat tacaggcgat tgttgccgga aacttcgatg aaactcaggt
    52681 gagcagtgcg gcgatgaaag cctggctggc gttctgggcc agcagtatgc atcagccgat
    52741 gctctatcgt ttacagcagg tcagcagccg ccgcttgctg tcgaatctgg tgagcgagtt
    52801 tcgccgcgaa ttgccgcgcg aacaggcaca ggaagcgggc tacggccttg ccgcgctgat
    52861 tgacggatta tggctgcgcg cggcactgag cggcaaaccg ctggataaac ctctcgcaca
    52921 ctcgctgacc cgtcacttta ttactcagca tttacccacc gattaaccga ggagacgtga
    52981 tgtcccgaat ggcagaacag cagctttata tacatggtgg ttatacctcc gccaccagcg
    53041 gtcgcacctt cgagaccatt aacccggcca acggtaacgt gctggcgacc gtgcaggccg
    53101 ccgggcgcga ggatgtcgat cgcgccgtga aaagcgccct gcaggggcaa aaaatctggg
    53161 cgtcgatgac cgccatggag cgctcgcgta ttctgcgtcg ggccgttgat attctgcgtg
    53221 aacgcaatga cgaactcgca aaactggaaa ccctcgacac cggaaaagca tattcggaaa
    53281 cctcaaccgt cgatatcgtt accggtgcgg acgtgctgga gtactacgcc gggctgatcc
    53341 cggcgctgga aggcagccag atcccgttgc gtgaaacgtc ctttgtgtat acccgccgcg
    53401 aaccgctggg cgtagtggca gggattggcg catggaacta cccgatccag attgccctgt
    53461 ggaaatccgc cccggcgctg gcggcaggca acgcaatgat tttcaaaccg agcgaagtta
    53521 ccccgcttac cgcgttaaag ctggctgaaa tttacagcga agcgggcctg ccggacggcg
    53581 tatttaacgt gttgccgggc gtgggcgcgg agaccgggca atatctgacc gagcatccgg
    53641 gcattgccaa agtgtcattt accggcggtg tcgccagcgg caaaaaagtg atggctaact
    53701 cggcggcctc ttccctgaaa gaagtgacca tggaactggg cggtaaatca ccgctgatcg
    53761 ttttcgatga tgcggatctc gatctcgccg ccgatatcgc catgatggca aacttcttca
    53821 gctccggtca ggtgtgtacc aatggcaccc gcgtcttcgt tccggcgaaa tgcaaagccg
    53881 catttgagca gaaaattctg gcgcgcgttg agcgcattcg cgcgggcgac gttttcgatc
    53941 cgcaaactaa cttcggcccg ctggtcagct tcccgcatcg cgataacgtg ctgcgctata
    54001 tcgccaaagg caaagaggaa ggcgcgcgcg tactgtgcgg cggcgatgta ctgaaaggcg
    54061 atggcttcga taacggcgca tgggttgcac cgacagtgtt caccgattgc agcgacgata
    54121 tgaccatcgt gcgtgaagag atcttcgggc cagtgatgtc cattctgacc tacgagtcgg
    54181 aagacgaagt cattcgccgc gctaacgata ccgactacgg cctggcggcg ggcatcgtga
    54241 cagcggacct gaaccgcgcg catcgcgtca ttcatcagct ggaagcgggt atttgctgga
    54301 tcaacacctg gggcgaatcc ccggcagaga tgcccgttgg cggctacaaa cactccggca
    54361 ttggtcgcga gaacggcgtg atgacgctcc agagttacac ccaggtgaag tccatccagg
    54421 ttgagatggc taaattccag tccatattct aaccaggagg tttatttgca atttgactac
    54481 atcattattg gtgccggctc agccggcaac gttctcgcta cccgtctgac tgaagatccg
    54541 aatacctccg tgctgctgct tgaagcgggc ggcccggact atcgctttga cttccgcacc
    54601 cagatgcccg ctgccctggc attcccgcta cagggtaaac gctacaactg ggcctatgaa
    54661 acggaacctg aaccgtttat gaataaccgc cgcatggagt gcggacgcgg taaaggtctg
    54721 ggtggatcgt cgctgatcaa cggcatgtgc tacatccgtg gcaatgcgct ggatctcgat
    54781 aactgggcgc aagaacccgg tctggagaac tggagctacc tcgactgcct gccctactac
    54841 cgcaaggccg agactcgcga tatgggtgaa aacgactatc acggcggtga tggcccggtg
    54901 agcgtcacta cctccaaacc cggcgtcaat ccgctgtttg aagcgatgat tgaagcgggc
    54961 gtgcaggcgg gctacccgcg cacggacgat ctcaacggtt atcagcagga aggttttggt
    55021 ccgatggatc gcaccgtcac gccgcagggc cgtcgcgcca gcaccgcgcg tggctatctc
    55081 gatcaggcca aatcgcgtcc taacctgacc attcgtactc acgctatgac cgatcacatc
    55141 atttttgacg gcaaacgcgc ggtgggcgtc gaatggctgg aaggcgacag caccatccca
    55201 acccgcgcaa cggccaacaa agaagtgctg ttatgtgcag gcgcgattgc ctcaccgcag
    55261 atcctgcaac gctccggcgt cggcaacgct gaactgctgg cggagtttga tattccgctg
    55321 gtgcatgaat tacccggcgt cggcgaaaat cttcaggatc atctggagat gtatctgcaa
    55381 tatgagtgca aagaaccggt ttccctctac cctgccctgc agtggtggaa ccagccgaaa
    55441 atcggtgcgg agtggctgtt tggcggcact ggcgttggtg ccagcaacca ctttgaagca
    55501 ggtggattta ttcgcagccg tgaggaattt gcgtggccga atattcagta ccatttcctg
    55561 ccagtagcga ttaactataa cggctcgaat gcagtgaaag agcacggttt ccagtgccac
    55621 gtcggctcaa tgcgctcgcc aagccgtggg catgtgcgga ttaaatcccg cgacccgcac
    55681 cagcatccgg cgattctgtt taactacatg tcgcacgagc aggactggca ggagttccgc
    55741 gacgcaattc gcatcacccg cgagatcatg catcaacccg cgctggatca gtatcgtggc
    55801 cgcgaaatca gccccggtgt cgaatgccag acggatgaac agctcgatga gttcgtgcgt
    55861 aaccacgccg aaaccgcctt ccatccgtgc ggtacctgca aaatgggtta cgacgagatg
    55921 tccgtggttg acggcgaagg ccgcgtacac gggttagaag gcctgcgtgt ggtggatgcg
    55981 tcgattatgc cgcagattat caccgggaat ttgaacgcca cgacaattat gattggcgag
    56041 aaaatagcgg atatgattcg tggacaggaa gcgctgccga ggagcacggc gggatatttt
    56101 gtggcaaatg ggatgccggt gagagcgaaa aaatgagtcg tgatgtgaac taacgcagga
    56161 accgatcaga ttgagagtta ccgttccaga gagggggacc gaatccttat ataaacactg
    56221 aggtaactct catgcttcat atccagtatt catgatgcgg gctttttgct gatatttaac
    56281 ccgaacattc ctgatacaga ctattaatac aaccttatct gcgacagatt ttattttctg
    56341 gatatattat gcgtgaacaa atcaaacagg atatcgatct gattgagatt ttattttatc
    56401 tgaagaaaaa gattcgtgtt atccttttta ttatggctat atgtatggct atggtgctgt
    56461 tgtttctgta tatcaataaa gacaatataa aagtgattta cagcctaaaa ataaaccaga
    56521 caacgccagg tatacttgtt agctgtgata gcaataataa ttttgcctgt cagactacaa
    56581 tgactgaaga tgttattcag cgaattacta cattttttca caccagccca gatgtcaaga
    56641 acagagaaat aaggctggaa tggtcaggag ataagagagc tttaccaact gctgaagagg
    56701 aaatatctcg cgtgcaggcc tctattatca aatggtatgc gtcagaatat cataatggca
    56761 ggcaagttct cgatgagata caaacgcctt cagcaattaa cagtgagctt tatacaaaaa
    56821 tgatatacct gaccaggaac tggtcattgt atccgaacgg tgatggctgt gtaactataa
    56881 gctcaccaga aataaaaaat aaataccctg ctgccatttg cctggctctg ggattttttc
    56941 taagcattgt aatttctgta atgttttgcc ttgtcaaaaa aatggtagat gaataccaac
    57001 aaaactctgg gcagtaataa acaacattct gatagagagt gcctgtataa agctgaagtg
    57061 acaatgaccg ggaagggaac ccgttttcag gaatggatgc ctgtcttttt cactgtaaat
    57121 atcctggcat tggtaatacc aggatattta cagaagagca gcataaccat gacggaacgg
    57181 ggttaagtca ggttgtccta aacgctattt tcaacctcgt atgccttctt caggtttatg
    57241 tccagacttc atatctctct caacaatcct caataatcag atacaccgct ttcaccgggc
    57301 catgaactcc gacgactttg ataagctcaa tatccgccgt tgaactgggg ccgctaatga
    57361 tgttaatgca ggaaggcatt cgttcaccgg cctgcgcttt ctgatgcaat ttttctgcga
    57421 gttgcgctac acgcggcagg atagtgcttt tacgcaggat aaaaagagaa tattccggga
    57481 gcaggctcaa tgaacgcccg cgctcggcgg cggaaaaaag aatcacgcct cccgattcgg
    57541 ttaaaccata ttcagcatac acaacaccca ctttagcctg ctctgcctgc gagatattct
    57601 cggcaccttt cgccggatcc caaacaacgg cattgcattc ctgctgcaaa cgttcgctaa
    57661 tccccaattc ctccagcctc gtgtcaccgc taatcacgac cgactgatct cccagctctt
    57721 tacacagacg tattgcagct tctgccgcct tcgcctcgct ggtcagctca cagcgcgtca
    57781 acataacatc gctggcaaac tgaataaacg cgtcacagcg ctgctgttgg ttaagttggg
    57841 taagccgctc gttagcatag ttgttaagcg gcgcatcttc tgcttgcggt tcaagtcgca
    57901 gcgggcgacc cagtgcctga gcaacgttat tcaaaaattc gcctcgatta tccattcttt
    57961 ttctcctgcg cctgatgttt cttaaaccaa ctacggaaac tctctccgtc agcttcagga
    58021 agatcgcgtg cttccatcca gtcgctaatc gcgccaaatt tgagtggtgt tttgccgcca
    58081 ttgataaacc agcttgccgc atgagcaccg gccatcatcc cgactttcca caatcctgga
    58141 tgactattgg cataagcgaa catttttatc gcccgttgct ctgcttttgc ggtgatccct
    58201 ttttcagcca tcacccgacg atgacgcaaa atcagttttg acagcggaat acgcaccgga
    58261 cacacgttgt cacaagctgt gcataaagag caggcgtagg gtaaatcttt aaaatcttta
    58321 tagccgccaa gtagcggaga aatcaccgca ccaattggcc ctggataaat agagccatat
    58381 ccatgaccgc caatatggcg atatgccgga caagtattca tacaagcccc gcagcgaata
    58441 cagcgcagca catcccgaaa ttcagaggcc agcacctcag aacgcccgtt atcgacaata
    58501 accagatgaa actcttcagg accatcaacg tgcccagctt cgcgcggtcc tgtcagccag
    58561 gtgttgtatc ccgtcaaacg tgcaccaacg gcactgcgcg ccagcatggt gatcaataca
    58621 tctacctcgg caaacgtggg ggcaatacgc tccattccca tcactgcaat atgcgtttta
    58681 ggcagcgtgg tacacattcg cgcattacct tcattggtca ccaggcatac cgaaccggtc
    58741 tctgccaccg cgaaattaca gccggtaata cctatttcag cactgaggaa atcttcgcgg
    58801 attttttgcc ggatgaataa ggtcatcgct tcaggcgttt ccggcccctc atagcccaga
    58861 cgttcgtgta gcactcgacg gatctgatgg cgatctttat gaattgccgg gaccacaaca
    58921 tgagatggcg gatcttgatc cagctggaga atatattcac ccagatcggt ttcaatcacc
    58981 tgaatgccag catcctgcaa cacatgattg acaccaatct cttcggtcac catcgattta
    59041 gatttcacca ccttccgggc atttttgcgt tgggcaacct gtaaaatgta gcgggtagcg
    59101 tcttctttgg ttcttgcaaa atagacgtga ccgccgtttt gcgtcacttt ttctgagagc
    59161 tggtacagat aagcgtcgag attactcaga acatgatcac gtatctgggc ggcccgatcg
    59221 cgccactcct cccagtgccc caattcatcg accatttttt gccgatttgc cccaatacgc
    59281 tgctgcgcgt ttgccaccgc tttgcgcatg atcggatctt caatttgctg acggatgcgt
    59341 gtcttaaaat ctgtattact ggttttgatc gacatcttta tatcctcagc ggctcatcaa
    59401 cacttcagca atatgcatca ctttgacttt ctgcccttcc cgttgtaatc gcccactgat
    59461 gtttagcagg caactcacgt cagcaccaat taaatactca gggcggactt ccatcaggtg
    59521 cgcaaccttt tctttcacca tctcgccgga tatttcggcc attttgaccg agaacgtgcc
    59581 gccaaatccg cagcaggtat cctgttcagc aaaggtaaac agctccagtc cacgcacatt
    59641 tttcagcagc gtaagtggct cgtccttcac tcccagctta cgggccaggc tacaagatgg
    59701 gtgatacacc gctctccctt gcaaactggc acctacatcg actaccccta atttattaac
    59761 aataaaagag gtgagatcct gcatacgcgc ggcaaccttt gcggcacgtg atgcccattc
    59821 aggttcatcc gccagataca tcgggtaact ttttacggca taggtgcagg agccagccgg
    59881 tgaaataatg ggatcatcgt tatcctccag cgcggcgatc agatttttca tccctggaat
    59941 cgcttctttg atataaccgc tattgatcgc aggctgaccg cagcatccct gtttctccgg
    60001 gaaatttacg cgacagccga gtttttccag tagcagcacg gagtctcgtg ccattcttga
    60061 tttcagggcg tcaccaatac aggtgacaaa gaaattgaca ttcaaaacta atactccatt
    60121 acttcatgcc catttatgcc cgatgtttcc cccccaccgg gggatcaatc atcaataaat
    60181 atggcgttct tttttaattg gctagcaaca taccacccct catttgtatg acaaatgata
    60241 atctttcatc caaacgtcaa atgcaataac taattttatt tctttcggca tcgctcacat
    60301 tttattggga aatcgacgat catttttggc ataagaaatc cattttaaaa tggctacatc
    60361 ccttatacct attcagagct acttattcag gggttattga gatatacact gatggtgtac
    60421 aaaaaaatca ccgacaataa tcacgtatca agtaatagtc gggattttat ttaattgaag
    60481 caatttagtt tgcattttta tttaaaaact gagctagagt ccatcagaaa aaaatatata
    60541 aagcccaaac caccgttgga aatagaaata gttgaaaggc tcacaaatcc cggaatgcgg
    60601 attatctttg ccctgatttc tggcggtttt attcagggtg caagctgtct gaccctttcc
    60661 cgatattctc ccggagtaca accaaactcg cggacaaacg ccttgtgaaa agatgattca
    60721 ctggcatagc ctactgactc agcgatcacc acaacaggga gcatttcccg ggaaaacatc
    60781 tgggccgcta tttgtagacg caactttgtt aatacagcca gcggcgtggt tccggaaaca
    60841 tcacggaaaa gctgggcaaa acttgcccgg gacatgtggg cgatgctggc cagcgattcg
    60901 acggtccagg cgtgtcctgg catttccaac atttgctgta ttaccgcacc aagacgtgga
    60961 tgcagaagca aactgagaat gtttttctca gtattaacct gtgcaatcca ctcacgcacc
    61021 gcgagggtaa agaatgtagc gcagatctgg ctacacagtg catccactcc cggcattgcc
    61081 aatctggatt cctgttgtaa aaacgggatc gcctccctca gccagttata ttctacgctg
    61141 tggttaaccg gtgctaaaaa cagcgtttcc ggcaaagacg ttaaaaaata acgcgccgaa
    61201 tgttgcagcc gaagagtgcc gcagacaata caggttgatt cattatcgac atgactcagg
    61261 cgatgggctg aattttgtgg tagcaggacc acatttcccg ggcgtaatgt aaaaatctcc
    61321 cccgtcggca tttccagctt cgccgctcct tgcgttaacg catgccaacg aataaccgat
    61381 aattccccgg caccatgtgg aagctgccag tcacttccta acacgcaatt cttatcgatc
    61441 gttccttgtg gagcgttaag catcaacaaa cggctaaggg catccatatc agactcctga
    61501 gtggtttttg attacaaaca ccaacaatct tagtaaataa ctaatctaat accatcaaat
    61561 agttaatcat gatttttgtt ttgtgattct gattaagaaa aagaaaatac cagacgataa
    61621 gacaaaatat caagcgttgt gaagaaatgt tatttgctct tttgcgtcta tagtcatgat
    61681 gtcaaatgaa cgcgtttcga caggaaatca tcatgaataa atatcaggca gtgattattg
    61741 gttttggcaa ggctggaaaa acattagccg tcacgctggc aaaagcaggt tggcgagtgg
    61801 ctctcatcga acaatcaaat gcaatgtatg gcgggacctg tattaatatc ggctgcatcc
    61861 caaccaaaac attggttcat gacgcacagc agcacacaga ttttgtccgt gccatacagc
    61921 gtaaaaatga agtggttaat tttttacgta ataagaattt tcataatctt gcggatatgc
    61981 ccaatatcga cgtgatcgac ggccaggcgg agtttatcaa taatcatagc ctgcgtgttc
    62041 atcggcctga gggaaatctg gaaattcatg gcga
//
LOCUS       DAFNBJ010000008        57702 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000008 DAFNBJ010000000
VERSION     DAFNBJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.008"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..170)
                     /locus_tag="L2X49_000518"
     CDS             complement(<1..170)
                     /locus_tag="L2X49_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN4795660.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMAS"
     gene            178..684
                     /gene="tnpA"
                     /locus_tag="L2X49_000519"
                     /pseudo
     CDS             178..684
                     /gene="tnpA"
                     /locus_tag="L2X49_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(702..1370)
                     /locus_tag="L2X49_000520"
     CDS             complement(702..1370)
                     /locus_tag="L2X49_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBN4795661.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1673..2266)
                     /gene="tehB"
                     /locus_tag="L2X49_000521"
     CDS             complement(1673..2266)
                     /gene="tehB"
                     /locus_tag="L2X49_000521"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBN4795662.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIVNMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2263..3255)
                     /gene="tehA"
                     /locus_tag="L2X49_000522"
     CDS             complement(2263..3255)
                     /gene="tehA"
                     /locus_tag="L2X49_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBN4795663.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3379..4359
                     /locus_tag="L2X49_000523"
     CDS             3379..4359
                     /locus_tag="L2X49_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HBN4795664.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4354..4890)
                     /gene="rimL"
                     /locus_tag="L2X49_000524"
     CDS             complement(4354..4890)
                     /gene="rimL"
                     /locus_tag="L2X49_000524"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBN4795665.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4952..5176)
                     /locus_tag="L2X49_000525"
     CDS             complement(4952..5176)
                     /locus_tag="L2X49_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBN4795666.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5316..6971)
                     /gene="opgD"
                     /locus_tag="L2X49_000526"
                     /gene_synonym="mdoD"
     CDS             complement(5316..6971)
                     /gene="opgD"
                     /locus_tag="L2X49_000526"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBN4795667.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7196..8539)
                     /locus_tag="L2X49_000527"
     CDS             complement(7196..8539)
                     /locus_tag="L2X49_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBN4795668.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEESSKRRCGLL"
     gene            8756..9679
                     /locus_tag="L2X49_000528"
     CDS             8756..9679
                     /locus_tag="L2X49_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4795669.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     VEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9717..11357)
                     /gene="trg"
                     /locus_tag="L2X49_000529"
     CDS             complement(9717..11357)
                     /gene="trg"
                     /locus_tag="L2X49_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBN4795670.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            11756..11905
                     /gene="hokB"
                     /locus_tag="L2X49_000530"
     CDS             11756..11905
                     /gene="hokB"
                     /locus_tag="L2X49_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBN4795671.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            complement(11977..12150)
                     /locus_tag="L2X49_000531"
     CDS             complement(11977..12150)
                     /locus_tag="L2X49_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795672.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12395..12925)
                     /gene="cybB"
                     /locus_tag="L2X49_000532"
     CDS             complement(12395..12925)
                     /gene="cybB"
                     /locus_tag="L2X49_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBN4795673.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            13114..14115
                     /gene="gap"
                     /locus_tag="L2X49_000533"
     CDS             13114..14115
                     /gene="gap"
                     /locus_tag="L2X49_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBN4795674.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(14157..15596)
                     /gene="aldA"
                     /locus_tag="L2X49_000534"
     CDS             complement(14157..15596)
                     /gene="aldA"
                     /locus_tag="L2X49_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBN4795675.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPTNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERIYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15793..16593)
                     /locus_tag="L2X49_000535"
     CDS             complement(15793..16593)
                     /locus_tag="L2X49_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBN4795676.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(16865..20767)
                     /gene="hrpA"
                     /locus_tag="L2X49_000536"
     CDS             complement(16865..20767)
                     /gene="hrpA"
                     /locus_tag="L2X49_000536"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBN4795677.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            20968..21573
                     /gene="azoR"
                     /locus_tag="L2X49_000537"
     CDS             20968..21573
                     /gene="azoR"
                     /locus_tag="L2X49_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBN4795678.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LVFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(21624..22940)
                     /locus_tag="L2X49_000538"
     CDS             complement(21624..22940)
                     /locus_tag="L2X49_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HBN4795679.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(22930..24687)
                     /locus_tag="L2X49_000539"
     CDS             complement(22930..24687)
                     /locus_tag="L2X49_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBN4795680.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRDIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24703..25587)
                     /locus_tag="L2X49_000540"
     CDS             complement(24703..25587)
                     /locus_tag="L2X49_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBN4795681.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFQYCTLISVHFPRWLYWVIPLNYLFIGFNCFELY
                     LLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPTTNIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(25587..26192)
                     /locus_tag="L2X49_000541"
     CDS             complement(25587..26192)
                     /locus_tag="L2X49_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBN4795682.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(26363..28669)
                     /locus_tag="L2X49_000542"
     CDS             complement(26363..28669)
                     /locus_tag="L2X49_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HBN4795683.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(28733..29593)
                     /gene="pdxI"
                     /locus_tag="L2X49_000543"
     CDS             complement(28733..29593)
                     /gene="pdxI"
                     /locus_tag="L2X49_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HBN4795684.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(29825..30415)
                     /gene="paaY"
                     /locus_tag="L2X49_000544"
     CDS             complement(29825..30415)
                     /gene="paaY"
                     /locus_tag="L2X49_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HBN4795685.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKLIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENLRPKQ"
     gene            complement(30397..31347)
                     /gene="paaX"
                     /locus_tag="L2X49_000545"
     CDS             complement(30397..31347)
                     /gene="paaX"
                     /locus_tag="L2X49_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HBN4795686.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVQLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPSPGSLYFQRFGGLNIEQEALCQFIR"
     gene            complement(31448..32761)
                     /gene="paaF"
                     /locus_tag="L2X49_000546"
     CDS             complement(31448..32761)
                     /gene="paaF"
                     /locus_tag="L2X49_000546"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HBN4795687.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(32788..33993)
                     /gene="paaJ"
                     /locus_tag="L2X49_000547"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(32788..33993)
                     /gene="paaJ"
                     /locus_tag="L2X49_000547"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HBN4795688.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(33993..34415)
                     /gene="paaI"
                     /locus_tag="L2X49_000548"
     CDS             complement(33993..34415)
                     /gene="paaI"
                     /locus_tag="L2X49_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HBN4795689.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(34405..35832)
                     /gene="paaC"
                     /locus_tag="L2X49_000549"
     CDS             complement(34405..35832)
                     /gene="paaC"
                     /locus_tag="L2X49_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HBN4795690.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(35834..36622)
                     /gene="paaB"
                     /locus_tag="L2X49_000550"
     CDS             complement(35834..36622)
                     /gene="paaB"
                     /locus_tag="L2X49_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HBN4795691.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(36622..37389)
                     /locus_tag="L2X49_000551"
     CDS             complement(36622..37389)
                     /locus_tag="L2X49_000551"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HBN4795692.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(37386..38456)
                     /gene="paaK"
                     /locus_tag="L2X49_000552"
     CDS             complement(37386..38456)
                     /gene="paaK"
                     /locus_tag="L2X49_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HBN4795693.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYARDHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIAATLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(38464..38961)
                     /gene="paaD"
                     /locus_tag="L2X49_000553"
     CDS             complement(38464..38961)
                     /gene="paaD"
                     /locus_tag="L2X49_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HBN4795694.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMSTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVCCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(38976..39722)
                     /gene="paaC"
                     /locus_tag="L2X49_000554"
     CDS             complement(38976..39722)
                     /gene="paaC"
                     /locus_tag="L2X49_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HBN4795695.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(39731..40018)
                     /gene="paaB"
                     /locus_tag="L2X49_000555"
     CDS             complement(39731..40018)
                     /gene="paaB"
                     /locus_tag="L2X49_000555"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HBN4795696.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(40030..40959)
                     /gene="paaA"
                     /locus_tag="L2X49_000556"
     CDS             complement(40030..40959)
                     /gene="paaA"
                     /locus_tag="L2X49_000556"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HBN4795697.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            41244..43289
                     /gene="paaZ"
                     /locus_tag="L2X49_000557"
     CDS             41244..43289
                     /gene="paaZ"
                     /locus_tag="L2X49_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HBN4795698.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RLFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLATGCEIRLGGQADLSAAGAFFPPTLLYCPQPDEAPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            43537..45810
                     /gene="tynA"
                     /locus_tag="L2X49_000558"
     CDS             43537..45810
                     /gene="tynA"
                     /locus_tag="L2X49_000558"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HBN4795699.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDSRDRVAPAVK
                     PMQIIEAEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(45868..47367)
                     /gene="feaB"
                     /locus_tag="L2X49_000559"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(45868..47367)
                     /gene="feaB"
                     /locus_tag="L2X49_000559"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HBN4795700.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            47603..48508
                     /gene="feaR"
                     /locus_tag="L2X49_000560"
     CDS             47603..48508
                     /gene="feaR"
                     /locus_tag="L2X49_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HBN4795701.1"
                     /translation="MNPAMDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESIN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(48680..49009)
                     /locus_tag="L2X49_000561"
     CDS             complement(48680..49009)
                     /locus_tag="L2X49_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBN4795702.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(49014..49199)
                     /locus_tag="L2X49_000562"
     CDS             complement(49014..49199)
                     /locus_tag="L2X49_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBN4795703.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(49196..51835)
                     /locus_tag="L2X49_000563"
     CDS             complement(49196..51835)
                     /locus_tag="L2X49_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBN4795704.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQSPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            52043..53032
                     /gene="ldhA"
                     /locus_tag="L2X49_000564"
     CDS             52043..53032
                     /gene="ldhA"
                     /locus_tag="L2X49_000564"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBN4795705.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            53143..53565
                     /gene="hslJ"
                     /locus_tag="L2X49_000565"
     CDS             53143..53565
                     /gene="hslJ"
                     /locus_tag="L2X49_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBN4795706.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(53562..53828)
                     /locus_tag="L2X49_000566"
     CDS             complement(53562..53828)
                     /locus_tag="L2X49_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBN4795707.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            54102..57626
                     /gene="nifJ"
                     /locus_tag="L2X49_000567"
     CDS             54102..57626
                     /gene="nifJ"
                     /locus_tag="L2X49_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBN4795708.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLIRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        14566 a        15161 c        13743 g        14232 t
ORIGIN      
        1 gaagccattt tcccgtaagg gatgtattta ttaccggcct catgattctc attctgacgt
       61 gccagagcac gattgaaaac gaaacgacat gcgcctgcga agcgcctcat ttcacgctcc
      121 tgttgaccac cgggtcttaa ctggaattta aatgcctgta gtcgtttcac aggccaaatg
      181 atactttggt ctgtgaaaaa agaaacagat atctggcgtg gaaggcattg tgttttcctg
      241 atgcatgttc acctggtctt tgtcaccaga taccgacgtc agatttttga ctatgacgcg
      301 acagaaaaac tacgcactta cttttcaaat gtatgtgctg attttgaagc tgaactggtt
      361 taaatggatg gcgaaccaga tcacgtccat ttgttaatta actatcctcc caaactggcg
      421 atatccagtc tggtaaacag cctcaaaggc gtatcgagta ggttactgcg acgagatcga
      481 ccagatattg cagtcaggta ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc
      541 agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca acagcaaaca
      601 cctggtcagg tggaaaaccg cgccttatat ccccgccctg aaggatgggg ttttacggcg
      661 caccggataa aaagcgccat ctaacgatgg cgcgtctgga actacttttt gttaacgtca
      721 aacatggtgg catcggtcgc catgtcatca ataacttgtt taatattttc gaaagccatt
      781 ggcgtacttt cgttattcag gtctttacct tcgccctgac gaacgacttt gataaccggt
      841 ttattagtcg ctgcatcaat cagctcacct tcaaaataga ggcgagtatc catggtacgg
      901 tggcctgtag ccatttgtgt ccccgcaacc actaatgcaa caggaaccac ttcatagaat
      961 tgcagccctt ctttgctggt atctacaccg gtaatggccc cacggaaaat cagactacgc
     1021 ggcccagcgg tggtaaccag tggtttacgc tgcgctatcg cttctttcat ttcggtgttg
     1081 gtatagttca aaattttatc cagaactttc tgccctacct gggtagaagg ttttggtacc
     1141 ggataataag tgattgggtt ccagacgatg ctgtcatatt tgctttgatc aaaactcggg
     1201 tctacccaac gtaaaacagg tttacctgtt gctgaggttg tttcttttaa atcagagtaa
     1261 ttgtttaaaa aaccagaata tttatctggc tgggtgattt tagaggcaca accagacaga
     1321 gccaacaagc cgcataaagc tgcaactttc gcaaatgatg tggtacgcat gaatagtttc
     1381 ctgagatgat tatattactt tctaagttat agcaaaaagc gccgcttcgt gttgtggcaa
     1441 aaaaagtgtt ggtgaaaata aatttttcag ccccttttca gacgacatag tcaatgccga
     1501 aaaaggggct aagtcaatca tcagtagtta aatttcagta agttaagctg ctaaaaaact
     1561 tcgactactg tgattaattt cacatccctt cgataaaaaa tatgcttacc gaaaagaaga
     1621 acagtaaatc atctcagcgt ggggaatttt cacgcgcctt attcttagcg ggtcattttt
     1681 tacgtgccag catcgtggcg aaacgcagtt taatacgatt accgttggcg tcggtgcggt
     1741 gcagttcgcc gacgtcttca ttgtatttca ccctctccca gccttcgtaa tatcgacgta
     1801 attctccctc tttgaaggca aacgggaagc cgacggtaca tggataatca gcggtatcca
     1861 tcgccgccac aatcaggttg taaccaccag gtttagtgca acgttgcata ttgacaatca
     1921 gccctgggat ggttttagcc tcaaggaaca tcagtaccac agtcgaaaga ataaaatcgt
     1981 actgtctatc aaatgtgagg ttattcagat caacgactcg ggtgtgtaaa ttatccagat
     2041 tttcaatgga tttaatgcgc tcgacgttgg cgatactcat ggcattttta tcccatgcgt
     2101 caacatcata accattggct gccaggtaaa gactgttacg accattgcca cagcccagat
     2161 ccagcgtttt acccggttta accactttca ccgcttccag tacttcagag tgtgtgcggg
     2221 ttaattcata tttatcagta aaatagtttt cgtcacgaat gatcattctt tgtcctctgc
     2281 tttcattaaa acggcgcgct cggttctgac taacaatttt ccctgcatca gaagcgcaaa
     2341 agtacggatg agcagtattg caataataaa attggtaaag ataaacagcg gcaccgccag
     2401 cgtatggaaa aatccattat cgctgccact ccccagatgc aaaccggtgg ttgccagtgc
     2461 agatacgccg aacgagaaac tccagaatga agcattaaat ggctgggaga gataccatgg
     2521 catcagacgt agcataaaca gcagttgcag cagtccataa ccgaaaagca ttttcgccag
     2581 cgtgtcaccc tcgccgccgt tgacgctcag ccaggcacta caagccacca gcgcaggagc
     2641 gagctgaatg ccgagtgatg tccgcagtgc cgtgggtaat tctcccgaac tgcgcagacg
     2701 ctgcaagatc accggttcaa ggcttagcca tgagaaaacg cctgcgccta aaaacaccag
     2761 accggcgtcg gtgtagccca acgcaccaca ggccattgcg ctgataaagt tgttggcaac
     2821 tgtcggcaga tacagtccag gcgtggtagc ttcttcaggg tgagatccgc gccataatcc
     2881 cgcagtttgc caggcggcat aagccaactg aaccacgaca ccaaaactga acaggcacac
     2941 cgccagtggg cgaaaccacg gaacaaaacc aatcgccacc agcatcgtcg ttgccggaaa
     3001 caaactcaca aaactgctca gcactggatg gcgaacttcc gccagcacgc tatgcggaaa
     3061 gcgtatgagt cgggtaataa atgcgctagt taataatccc cagatgatca tcgccagaat
     3121 caccagccca tcccctaacc agtggctgac ctgccaaacc tggctggcat agcgccaggc
     3181 aaatcccatc cctatcgtcc ccaacacaat accaaagtag cctgccggca aattgagcac
     3241 tttatcgctc tgcatcttct tactcatttt gtttaattta taaagtatat ttgaaatgca
     3301 ttttatggaa ttttctcgag catagccaga gccgcagaat ttgctacggt tatagtaagt
     3361 cacctgtggg aaaacgctat gcgcaaatat cgtctaagtg aagaacagcg agcctttagt
     3421 tatcaggaag atggcactaa aaaaaatgtg ttattacggc agattatcgc cattagcgat
     3481 tttaacgatg tgatagcagg aaccgctggc ggctggatcg atcgcgaaac ggtgttagca
     3541 caggaaggaa attgctggat ttacgaccag aatgctattg cgttcggcgg aacggtgatt
     3601 tccggtaata cgcgcatcac cggcaccagc gtactgtggg gagaggttta cgcaacggat
     3661 aatgtctgga tcgataatag tgaaatcagc cagggtgcat acataagtga cagtgtcacc
     3721 attcatgact cattagttta cgggcaatgt agaatatttg gtcacgcctt aattgatcaa
     3781 cattctatga tcgtcgccgc acaaggctta acgcccgacc atcaactcct gctccaaatt
     3841 tatgatcgcg caagggttag cgcctcgcgt attgtccacc aggcacaaat ttatggcgat
     3901 gccgtcgtcc ggtatgcttt tattgaacat cgcgccgaag tttttgattt tgccagtgtt
     3961 gaaggtaatg aagagaacaa cgtctggcta tgcgattgcg ctaaagtcta cggtcacgcg
     4021 caggtgaaag ctggcataga agaagatgcc attccgacga ttcattacag ttcgcaggtg
     4081 gcggaatatg ccattgtgga aggtaattgt gtgttaaaac atcatgtctt gattggcgga
     4141 aatgccgtgg tacgtggtgg accgattctg ctcgacgagc acgttgtaat acaaggtgag
     4201 agccgtattt caggtgcggt gataattgaa aaccatgtgg agttaaccga ccacgccgtc
     4261 gttgaagcct ttgatggcga cacggttcat gtccgtggtc caaaagtcat taacggcgag
     4321 gagcgcatta cgcgaacgcc gctggcaggg ttattatgaa tcgataatac gcgcgtataa
     4381 gttcacatca tcataggcat cattcaggaa ctcagcctgt ttcaggcaac cttcaaggat
     4441 aaaaccattg cgcaaagcga cctggttgct ttgcggattg tccacccgac atttgatcac
     4501 gaagcgtcta agttcaccag actgagcgta atgatgaatc aatgcctgca gcgcctgaga
     4561 aatgatcccc tgcccctgat gagattcgtc cagccagtag ccgatttcag cagttttatt
     4621 cagtggttca atacgattaa acgagataac gccgataagt tcatcttctt tgaaaatcat
     4681 gaacattttg gcatagccgc gttgatgcaa catcacatta ccctgcaccg tttttcgcgt
     4741 gtcctcttca ctctgaacga actgcggcca gtttagcgac tgctgtaacc aggttttatt
     4801 tttacagatt aactgataaa gaggtgtgac gtgactttcc gcaacagcat gtaattcaag
     4861 tgattcgctt acttttatcg tttcagtcat cttatctcca gtcaatccat gctgttgtta
     4921 aaaaagccgc ctggctggcg gcttaggaaa gttacacctc tttgacgctc tcctgctgca
     4981 ggattttgag catctcatct tttaactgta gcttttgttt tttcatgcgg accacttccg
     5041 cattgtaccc tcgaccgtcg gaaccttcct ttctggcaat ttcatgatca agtttattgt
     5101 gtttatcgaa caaggacata aagcgaggat tttcgttttt cagacgggat attaaatctc
     5161 ggtattctgg aaacatacgc actccctgtt agtggctgac aaggtgtatc tgaaatacac
     5221 accatcagat tactcattta acttagggaa aaatgcgagc gggatcgtgt ttaaccagac
     5281 ggcacctccc gaagaaggtg ccgaagaaaa aacgattaac tcatcacgcg gtcgtcaaca
     5341 tactgacgtt tatccggcgc tggcgggaaa tactgataca gccatgtttc actgatagcg
     5401 tcgccctgac aacgcagata catccgcata tcgaccggat cagtggagtc cgaagtcgga
     5461 taccagtcaa actgaatacg ataaccatca atgggttcaa tatagagaat ttcgatttgc
     5521 ttcgcttccc cactggaaag cgtaatcacc ggctcaatgc cttttggtgc ggcagctttc
     5581 agatcaccac caacgaaatc gacggcaaaa cgacgcgccc atttttcggg atagtgttca
     5641 cctggtgccc aaccttccgg gaaaccgccc atgccggtac gcgtcgccat aacgcgcgct
     5701 aatgggcaat gaacaggcgg ttgcgcactc cagtacagac gatactggaa tgcaaactca
     5761 tcacctgctt ttacagcttt ttctggctgc cagaagcaga caatgttatc cagcgtttcg
     5821 cccgttgttg ggatttccat caggccgatg gtgcccttac cccacttgtt acgcggctcc
     5881 acccacagac ttgggcgttt gttataccag cccataatgt cctgataatg ggagaagtca
     5941 cgatccagtt gcaataaacc aaaccctttc gggttgttgt cggtgtaagc attgaactgc
     6001 aatttttgcg gattattcag cggacggcaa atccactcgc cgttgccccg ccacatggac
     6061 agacgatcag agtcatgaat ttgcggatga attgtatcgc acatccgacg ttcattagta
     6121 ccgcagctga acatactggt catcggcgca atgcccagct gtttaatgtc tttgcgcgca
     6181 tacaggtgat tttccacatc cataatcacc tgacttttct cacaatggat agtgaactta
     6241 taggcaccag taatgctggc gctatcgagc aacgcataaa cggtaaaggt agttgccccc
     6301 ggttttaccg tatcaaacca gaaggcggta aagtcgggga actcttcttt actgtcagtg
     6361 taagtgtcga tcgccaggcc gcgggccgac aaaccgtatt gatatgtatc atcaacggcg
     6421 cggaaataac tcgcgccgag aaatgatact acatcacggc gcgccagttc gggggcttta
     6481 aacacgcgaa aaccggcaaa gccgagatcg ctttgccctt ctaattgttt tgtatcaaca
     6541 cccgcatcgt tgtatttgaa caactccggg cgaaagtgaa tttcacgcgc cagatgtgtt
     6601 gctggatcta cagaaaacat acgaacgcgg cgacggaatc ccattcccat atggaagaac
     6661 tgagcgtcca gttgacggtt ctcaacgtta tgccagagcg atttttcggc gtcgtattga
     6721 atactgttat aagcctgcgg cgtcattgtc gccagcgtgt caggtaacgg acgaggcgca
     6781 ccacgccacg ctgtttgcgc taagtcgtgc gccattgact gtagaatgga gaagtcaaaa
     6841 cgctgggttt gcccgtcggc aatatcagaa tctgccgcga atgccgcctg agaaaaaaga
     6901 gaagcaatgc cgctggtacc gcacacggcg gccatagcca ttgaaccttt aataaatcgt
     6961 ctacgatcca tacctgaaag tgagtccttc tggtgttgtg aatggtcccg cgctggtcgt
     7021 tttcagcggc aagaaagtgc ttatttatga ccgctcactc tagacaatat ttattaataa
     7081 tccaattgtt gaactgagag aatatgcata aatcggcatt ttttttatgt cgatgggatg
     7141 agcctgattc gtttgtaaaa tcaggcaaat ttttgcaaag gcggatggtc agaatttaaa
     7201 gcaaaccaca gcgacgttta ctgctttctt ccgcttcctg gtaaagctgg aactcatcca
     7261 aaaccggaca acccaacgcc tgctcaaatg cttcgcgact ggcattaccg tgactgcgtg
     7321 tctgcgtttc attacccaga tttgactgga agatccccgc cgcgctaacg ggcaagaaat
     7381 cttcataggt aatgggttgc gccgcgaccc aaccacgttc aattaagggc tgtggatcgt
     7441 ctccgggatg aatcgcctga cgatgcgcct cacctgaagg cgtcagacgg taccggaacc
     7501 atgccagccc ttgctgacgc attaaaaact cactgtcagg aaaagtgcgg aaggtttcct
     7561 gtaaatgcat ttggtgagtg agattatcct gcccggttcc agcgttacgc agaagatcat
     7621 catacagttg tcgccctttc ggcgttaatg ccacgccacg ctgctcaatt tcaccaaagc
     7681 gcgcggtatg cgtgccctgt ttctgccccg caaacaacac cgtctcttcc agtgctttaa
     7741 agctggtctg gcgtagtaaa atcggtacct cgcggcgcgg cggcccctcg atgagaattt
     7801 tgggttcaat tccgcattca ggcatcatcg actgcacccg gtcaatatcc agcgtacgtg
     7861 gtgtcaggtg gttgatatgg catccaggaa aacagaccac atcagcaatc aaccgatgtt
     7921 cgttgtgcaa ggcgcgatag gtttcttcat ctaccgttgc tgactggtgc cagcgaaacg
     7981 tttccagggc ttcctgcaca aactcctgtg cctgtgtttc gttaaaaccg ccctgctgct
     8041 catattcctc taacagttgt cgacaacgtg gggtgaagat atcgcgctga cgcagaatct
     8101 ccgccgcttt ctggcgcaaa atttcgttct cgataagctc aaggcggagt aaggaggtaa
     8161 aaacgcggaa gggattacgc gccagagaag catcatcaat gggccgaaat gctgtcgaat
     8221 gtaccggcac ccctgcctgc gagagatcgt aatagctcac cgggtacatc cccataatgg
     8281 caaacatccg ccgaagagta gcaagctctt gtgcagtccc aacgcgaatc gccccatgac
     8341 gttcaacatt cagtcgcgcc agctcgtctg catttaccat tttttcgtgc agttgaggat
     8401 tgttttccag cacagccaga ttcacatcag ctaccagttc cagcagcgtg ccatattgcg
     8461 gaacttcttg ctggtacatg gctgacattg cctgcgaaaa ctgttcccga atctcatccg
     8521 ccgtgatgct gttcgccata gtgtctgcct ccagtggtca gtaatctgga gtgtaggtaa
     8581 ccgcattcac tcttgcggga agaatttaca aactgtgatc tcgccgcgaa aacatcaata
     8641 ttatccattt tgctgtaaca taattgcttt aattgttaat aatattttgc aatcaagtta
     8701 tcataatcaa acaacttcac ttgtcagcga caccgcttcg tttttaacat cgcttatgga
     8761 aaaaaatagt ctgtttagtc agcgcatccg tttgcgccac cttcatacat tcgtagctgt
     8821 cgcacaacaa ggaactttgg ggcgcgcggc tgaaaccctt aatttgagtc aacctgcgct
     8881 ctctaagaca ttgaatgaac tggagcagct gactggcgct cgcttgtttg agcgtggtcg
     8941 tcagggggcg caacttacct tacccggcga acaattttta acgcatgcag tcagagttct
     9001 tgacgccatc aacactgccg gacagtcgct tcatcgtaaa gaaggtctta ataatgatgt
     9061 cgtcagggtt ggtgcactac ctactgcggc actggggata ttaccttcgg ttataggtca
     9121 gtttcatcag caacaaaaag agacgacctt gcaagttgcg acaatgagta accctatgat
     9181 tctggcgggt ttaaaaaccg gggaaatcga tatcggcatt ggtcggatgt cagatcctga
     9241 actgatgacc gggcttaatt acgaactgct gtttcttgaa tcgttgaagc tggttgtccg
     9301 ccctaatcac ccgctacttc aggagaacgt aacgctaagc cgggtgctgg aatggccggt
     9361 cgttgtatca ccagaaggca ctgcgccacg ccagcattca gatgcattag tgcagagcca
     9421 gggctgtaaa attccttcgg gttgtatcga aacgctgtct gcttcgctat ctcgtcaact
     9481 tacggttgaa tacgattacg tgtggtttgt cccttctggc gctgtaaaag acgacctgcg
     9541 tcatgccacg ctggtggccc tgcctgttcc gggacatggt gcagtcgaac cgattggaat
     9601 actgacccgc gtagatgcga cgttctcttc tggttgccag ttgatgatta acgctattcg
     9661 aaaatcaatg ccgttctgaa aggtgaaggg atctgtcgat ccctccttga acattttcac
     9721 accgtagcga aactaactgg ttcacccgct ccgcgaggtt ctgccgacac agaatgtttg
     9781 tgcagacgga atacatccac cgcctcagtt aatcgtgccg cctgttcttc aagggacacc
     9841 gccgccgctg aggcctcttc taccagagaa gcattctgtt gcgtcacctt atccatttca
     9901 gaaatcgcct ggctaacctg cgttatgcct ctactttgtt catccgaggc ggcggcgatt
     9961 tcctgcatga tatgtgtgac actcgcgacg gcatcaacaa tagtgctcat ggttttcccg
    10021 gccgttgcca cctcatccga ccccaggtca attaacctga ctgattcact gatcaagcct
    10081 tcaatctctt tcgccgcctg agcgctgcga cttgcgagtg tccgtacttc gctggcgaca
    10141 acggcaaatc cacgcccttg ctcacccgct cgcgcggctt caacggcagc attcagtgcc
    10201 agaatattcg tctggaaagc aatactgttg atgacggcgg tgatctcaga aattttcttc
    10261 gaactcgtgg agatagcgcc catcgttttt actacaccgg aaaccgtctg cccgccatcg
    10321 ctggctttaa tagaagcctc ttgcgccagt ttgctggcat gatgcgcgtt atccgcattc
    10381 tgtttcaccg tcgcagtgag ttgctccatg ctggcggcag tttgttcgat agccgccgct
    10441 tgttcttcgg tgcgagatga caggtccgca ttgccagctg aaatttcgct ggtgccacga
    10501 taaatctctt ccgcgccctg tcgaacagtc cctactgtca tccccagtga atgctgcatt
    10561 tgctgtaaat gacgacttaa gcgaccgatt tcattacgac ccgccggttc atcattcatc
    10621 gtcagatcgc cactggcgat tttttcaatc cgttgtgcgg catgttgcag tggacgaatg
    10681 acgatccgac gtagcaccat aaatgttatc agcgtcatga ccagggcaag cacaaacgcg
    10741 ccaatcatga acatcccacc caggcgggtg cgctgatggg ccagttccgc cagttggttg
    10801 gctctggtgc tacgtatctt aacggctttg ttcaaaatat cggtataagc attatccagc
    10861 ggtcggatct gctcactttc atgattgata atcgcttcaa acatgccatt tttggcatat
    10921 ttcaacatag gttgcatacc cgtgatataa gcctgaaagc gttgatttaa ttcagtgtcg
    10981 agggcttcat cagcaggtgt tttcaccggt cgattctgat aagcacgata accttgctgc
    11041 gactgtttaa tctccgattc ggcttgcgca atatttcgct tcattgcttc catttccgca
    11101 atacgactcg cagccccggc ctgaatcata ttgatccggg ctgaacgcaa aaaatctgaa
    11161 ctgttcgcta acccggtccg aatctcaatc tccgcagtaa catcacgctg atcgcgatcg
    11221 gcctgccaga ggaaatagcc agccagagca gaacttaatg cgaatagaac taagataccg
    11281 ccaagaatgc aggcaaataa cggaaccagc ctgatgtgat gcaaaaaacc taatcgctgt
    11341 gagggagttg tattcattac cgttgtctct cgtccaggtt tactcctgat agaaagtcat
    11401 cggcacttga cggtaattac ttatgaccaa aaacctcttt gcgtcacatt tttacaacaa
    11461 ttattgcaga gaaaattaat gctattggaa tgaagagagt aacgaatggc taaactggct
    11521 aatccgaatg acgaaaaatt ctggaaaaca gcacgcaatt tcgtgaagtg attattttcg
    11581 tccctaagga aacctcatag gatgcctgcg ggtgcctgag actttctgtt ttcaggctaa
    11641 ccgggaatca gaaagacgaa ggccccgacc gatatttcta tcaaccgagg ccaacgaatg
    11701 aacctagcaa ttcagatctt agcctcttac ccgccatccg gcaaggagaa aggctatgaa
    11761 gcacaaccct ctggtggtgt gtctgctcat tatctgcatt acgattctga cattcacact
    11821 cctgacccga caaacgctct acgaactgcg gttccgggac agtgataagg aggttgctgc
    11881 gctcatggcc tgcacgtcca ggtaagggca agcgcgggga ttttccccgc gcatctgact
    11941 agcagattca ttttctcatg gcaccccttt tacacttcag ttagtgcatg tttttttcga
    12001 ggcactaata gaaccatcat tacagacaaa tttgccgcct gccgtacagt gtgagatccc
    12061 ccctttcgaa ccagaacagg gtttgcgccc ggcatcggca taaaaggaaa caagcgttcc
    12121 cataaacaaa ataagtgcca gttttttcat ataacctcca tggtttatcg aatcttatgt
    12181 tcgatttgaa cgttatacgc tggtttattt tgataccgtt gatgagatca aagatcgggt
    12241 gaattgatta aaaataagcc agatggacgt atgggcaagg attatggttt tatttgtcat
    12301 acaaataaat ataataggcg cttccgatgt agacccgtat tcttcgcctg taccacgggt
    12361 cggttttagt acaggcgttt tctttaaata tccttcagga acgtttacgc ggcatcatgc
    12421 gtagaagtgt gttgtccttc cagaaatagt ggtgtgccag tgcagccgca gcgtgcaacc
    12481 cgatgacaaa atatcccaga ttcgccagcg tctcatgcca cgactttaag ctatcaaccc
    12541 gttcgaaatt ggcctctgaa gcgtaaggca tcgtcaaacc aaacgcaaac cacgggttgc
    12601 cccggttata catcatcacc aaaccaatca ctggcagcgc aataaacagg agataaatca
    12661 ccaaatgtcc caaatgcgcc agtcccgtca tcatcggttt tggcttaggt ataatcggcg
    12721 gggttgggta tttcagtctt aacaaaagac gaaccaccat cagcacgaga attgagatac
    12781 cacaggaaac atgaaccatg ttgatgagtg gccgatcgct acgtgggaag aaaccacgaa
    12841 actccattgc gcaatacgct gcgataacca gcaaaaagac cagccagtga atgctgattt
    12901 gtaaccttga gtatttattt tccataacat ttcctgcttt aacataattt tccgttaaca
    12961 taacgggctt ttctcaaaat ttcattaaat attgttcacc cgttttcagt acgttacgtt
    13021 tcactgtaag aatggagatt gcccatccca caatcctggt ctaagcctgg aaaggatcaa
    13081 ttttcatccg aatgttcctg acaggagaaa accatgagta aagttggtat taacggtttt
    13141 ggtcgtatcg gtcgactggt gttgcgtcga ttacttgaag tcaaaagcaa catagacgtt
    13201 gtcgctatta atgatctcac ttccccaaaa attctcgcct acctgctgaa acatgattca
    13261 aactacggac cattcccctg gagcgttgat tttacggaag attcacttat cgttgatggg
    13321 aaaagtatcg cggtttacgc cgaaaaagag gctaaaaata ttccgtggaa agcgaaaggt
    13381 gcagaaatca ttgtcgaatg tactggcttt tatacctccg ccgagaaatc gcaggcgcat
    13441 cttgatgctg gtgcgaagaa ggtgttgatt tccgcccctg ccggtgaaat gaaaactatc
    13501 gtttataacg tcaatgacga cactctggat ggcaacgaca ccattgtttc cgtggcgtca
    13561 tgcaccacta actgtcttgc gccgatggcc aaagccttgc atgacagttt cgggatagaa
    13621 gtcggcacga tgacgaccat tcatgcctat actggcaccc agtcactggt ggatggccca
    13681 cgtggtaaag atttacgtgc ttcacgcgca gcggcagaaa atatcattcc ccacactacg
    13741 ggggcggcaa aagccattgg tctggtgatc ccggaactga gcggcaaact gaaaggtcat
    13801 gcgcaacgcg tgccggtgaa aacaggttcg gtcactgaac tggtatcgat tctcggaaaa
    13861 aaagtgactg ccgaagaggt gaataacgca cttaaacaag caaccaccaa taacgagtca
    13921 tttggttata ccgatgaaga aatagtctct tccgatatca ttggcagcca tttcggttcg
    13981 gtgtttgatg ccacgcaaac ggaaattacc gccgtgggcg atttacaact ggtgaaaacg
    14041 gtcgcctggt acgataacga atatggcttc gtcacgcagc ttattcgcac cctcgaaaaa
    14101 ttcgctaaac tctgacgcgc acaggcggag gaaaaaacct ccgcctcttt tactcattac
    14161 gactgtaaat aaaccacctg ggtctgcaga tattcatgca ggccatgttt accatctgcg
    14221 ccgccaatac cggatttacg ccatccggcg tggaagcctt gcatagcttc gaagttttca
    14281 cggttgatgt aagtttcacc aaacttcagc cctttaatgg ctttcatcgc gacgttcaga
    14341 ttttgggtat agattgatga ggtcaggccg taatcactgt cattagccat tgagatagcc
    14401 tcttccagcg tgtcaaatgc gaccaccggc agaaccgggc caaaggtttc ctcatgcata
    14461 atcgacattt cctggcgaac atccagcagc aatgtcggcg gataataata tcctttcccc
    14521 tctaccgctt tgccacccaa cgccactctc gccccttctt ctactgcgcg cgccactttt
    14581 tgctcaaccc tttccagcgc cgcggcgtta atcaacggcc ccatcgcaat gtcgttgcgt
    14641 tcagcggggt taccaaattg aaccgcctgc atcgcttcac ccagccgatt gacgaactga
    14701 tcataaatgc ctttctgtac ataaatacgt tctgcacagt tacacacttg cccactatta
    14761 atgacgcgtg aatcaacgat ggctttgact gccagttcaa gatcggcatc gtccattacg
    14821 atagctggtg ctttaccccc caattccaga cacactttgg tgatgttttt cgccgcagtc
    14881 gccatgatct tctcacctgc agagacgctg cctgtcatac tgaccattgc gacctttggg
    14941 ttacccgcca gttcttgccc aacggtttca ccacgcccca gtacaaggtt aaacacgccg
    15001 cgcggaaggc ctatttcatc gacgattttg gcgaatgcaa tcgcattggt tggcgtaaat
    15061 tcactaggtt taatgacgat ggtattaccg gtcaaaagag cgggagccat tttgcgggca
    15121 atgaggaaga acgggaagtt ccacggcaga atgccggtag tcacaccaag cgcacgttta
    15181 aacaaaagaa tattttctcc tggacgatcg ctttgaataa tctcgccctc gtaacgccgt
    15241 gcccactccg ccatgtaatc gatatagtcg gcagtaaaag ccacttcgac ttcagccagc
    15301 tgctggatct tgcccccttc ttcaacaatc agcgcactga tttcactggc gcgttcgcgg
    15361 atcccggcgg agattttgcg caaccaactg gcgcgttcaa tagcaggcaa cgcttcccat
    15421 tctggttgtg cacgttctgc tgcatcgatt gccttacggg catcctcggc ctgaccatcg
    15481 ggtatgcggg aaatgacagc ctctgtagca gggtttacca catcaatcca tgcgtctcca
    15541 cgccaggtaa caaactgtcc atcgatatac ataggatgtt gaacgggtac tgacatgggc
    15601 gactcctgtg atttatatgt ttgttttcgg tgaatgcatt gttaattaac atcaatcatc
    15661 agagagacgg catcgtgaaa ggacaattct gtgaagggct tcataaaaac aagtgtaaaa
    15721 ggaataaaag gcagaaataa aagtcaggag tcatgcccgt ttgcaaacaa gtggttcaca
    15781 aacgggcaat ttttaacgta acgagcgact ttccatcgcc tcgatgagca ccgcatcatg
    15841 tttcagcgtt tgccatgcat ggatgacttc tgccggaaaa tcaacgtgaa cgataaaatc
    15901 tcgcccgcgg ggaccgtagc catcgctatc atcgcgtaaa cgcggcagct cgccagtgag
    15961 tagtgagaga taacgctcaa ccggccataa tccttgtaac tgattaataa agattacact
    16021 gtctgcgtta tttcctaact gaggaacgaa tccgggataa cttaaccagc gtggtgcatc
    16081 gggattgtcc ccagtcatac ggcggaacgt cgccatcgtg cgccgctgca tggtggggtc
    16141 ctgaacaacg atagccgtat gaactcgttc tacggcctgt ttcaatagcg cgatgctaaa
    16201 gcgtgcgttt tcaccgcagt ttgttgactg gtcttcaatc cagatttttt catgcggaat
    16261 gtgccagaac tgatgagcga tatccgccag gatggtcgct tctgctctgc cagtggtgcg
    16321 gatagtgttg tagtgcggat gctgtgcgat ggcgctatac aaaaaagttg tcgagtgacc
    16381 gataccacca ctaatcagta aaggaatttg ctgatcgcga gcaatcttac atgccgcgtc
    16441 gatagtcggc ataaccgcat tgcctgcaag gatcacgcaa tcggcctgat acggcacctc
    16501 accggagaaa tcatcctgcg ccagccactg tccgataaca tttatggcat ctatagttgc
    16561 cggcgaaagc gtcggaaacg gggtaatgtt catggttcct ccttatcgtt cccttaagaa
    16621 tactgcgtga gataatagtg agaagcggag cactctgaaa ggaggaagtc gtatgtcctt
    16681 aaccgccact ccgcaagcct gacatatctt ttaaggaaat gctgtagata aacattattc
    16741 atcttgcagc agagaattgt atttttctga attcctgaat ttttaccctt accgttttag
    16801 aattaataaa aaacccggcg ctaaatgctt accgggtttt ttctttatca ggcaaatagc
    16861 agggttaacc gctaatctgc tccatcgcct gcaaaatacg cttatctgaa atcggataag
    16921 gcgtaccaag ttgttgagcg aagtaactaa cgcgcaactc ttctatcatc caacggatct
    16981 ctttcacgtc ttcatcctca cgacgtgcag gcggcagttt gttgatccat tgctgccacg
    17041 cctgctggac gttttcgact ttcagcatct gagcacggtc gcgatgtgga tcaaccgcca
    17101 gtttttccag ccgtttttca atcgcctgca aatatcgcag cgtgtcgccc agccgtttga
    17161 agccgttacc agtgacaaat ccgcgatata ccaacccgcc catctgcgct ttaatgtcag
    17221 aaagccccag tgccatggtc atatccaccc gccctttcag acgtttgttg atattgaaca
    17281 ctgccgtaag aatttgctcg acctgcttcg caatatctac caccgtgtcg ttcagttcgg
    17341 cacgcacttt ctcatgcagc gcagcaaagc cttcttccgt ccagaccggg ccaccattgg
    17401 cgtcgatcag ttgatccaca ccgcaggaga tacagtcgtc gatcagctcc agcactttgc
    17461 catacgggtt aaagtacagt cccagcttgg ctttgtttgg taacttctca tgtaaatact
    17521 tgatcggcga gggaatattt agcagcagta gacggcgaag accgttccac attgcctgtt
    17581 tttgctccag cgggttatca aacagtttga tcgccacact gtcacgttca tccaccagcg
    17641 ccggccacgc cttcacttta tagttgccgc gcttctgttc gtagctttcc ggcagttgcc
    17701 caaagctcca gatatgcagc ccgctctgct cgataccgtc atccgccacc gcggacagcg
    17761 tttcctgcac tttgcctttc agcgcatctt tcagatcctg taatgagcgc ccttctttta
    17821 gcttcttatt tttgtcgtcc accacgcgga aggtgatttt caggtgatcg ggcacctgat
    17881 cccagtgcca gtcttcgcgg tcaacggtaa cgccggtcat ccgccgtaac tcgcgctcaa
    17941 ggctgtcgag caacggtaac tccagcggtt tgacgcggcc taaaaacgct tcggcatagt
    18001 ttggcgcggg tacaaaatta cggcgtaccg gtttcggcaa cgatttaatc agagcaatca
    18061 ccagttcgcg gcgcagaccg gggatctgcc attcaaaccc gctttcctca acctggttaa
    18121 gtaacggcag cggaatatgt acggtcacac cgtcagcatc cgcgccgggc tcaaactgat
    18181 agctcaaacg cagcttgaga ttgccctgat gccagaagtt cgggtaatcc agcttgctga
    18241 ttttttctgc gccctctttg atcaacatgc ttttttcaaa gttgagcaaa tcaggcgttt
    18301 cgcggctgac ttttttccac cagctatcga agtggcgcgc agagatcaca tcgtggctga
    18361 tgcgctggtc gtagaactca aacaacgttt cgtcatcaac cagaatatcg cggcgacgtg
    18421 atttgtgttc cagctcttcc acttcggccc gcagtttcag gttttcacgg aagaatgcgt
    18481 gacgcgtctg ccagtcacct tccaccagcg catggcgaat aaagagttca cgacataacg
    18541 ccggatcgat ctggctgtag ttgaccttgc gcgcggcaac aatcggcaaa ccataaacag
    18601 tgactttttc cgttgccatc accgcgccct gcgcccgttc ccagtgcggt tcgctgtagg
    18661 tacgtttaat caaatgctga gcgacaggct ccacccattc cgggtcgata cgcgcagcaa
    18721 tgcgccccca caggcggctg gtttctacca gttccgccac cattacccat ttcggcggtt
    18781 ttttgaataa accagaaccg gggaagatgg agaaacgcgc gttacgtgcg ccggtatatt
    18841 cttgtttatc ggcatctttc atgccgatat gggaaagtaa acccgtcagc aacgcaatgt
    18901 gaatttcgcg atactccgcc ggttcgctgt taaccggaat gccaagttct ttcaccacct
    18961 ggcgcaactg ggtgtagata tcctgccatt cgcgcacgcg cagatagttg agataatcgg
    19021 tacgacacag gcgacggaag gcgttggaag aaagcgcctt ttgctgctcg ccaagataat
    19081 tccacagatt cacaaacgcg aggaagtcag actctttgtc gtggaagcga cgatgttttt
    19141 cgtccgatgc ctgctgttta tccatcggac gctcgcgcgg atcctgaatg gaaagcgcgg
    19201 acgtgataat catcgcctca cgcacgcagc catgtttttg cgcttccagc accatacgcg
    19261 ccagacgtgg atcgacaggc aactgcgaga gctggcgacc gagcggcgtc agtttatagg
    19321 cgctggcctg ttcatccgtg gtgatcgcgc ccagttcttc gagcagacgc acgccatcct
    19381 ggatattgcg tttatccggt gcttcgacaa acgggaacgc agcgatatcg cccagcccca
    19441 gcgcggtcat ctgcaaaata accgaggcca ggttggtacg cagaatctcc ggatcggtaa
    19501 actccgggcg cgaaaggaaa tcgtcttcgg aatagagacg aatacagatc ccttcggaca
    19561 cacgcccaca gcggccttta cgctgattgg cagacgcctg cgaaatgggt tcaatcggca
    19621 aacgctgcac tttggtgcga tagctgtagc ggctgatacg tgctgtaccg gggtcgataa
    19681 cgtatttaat ccccggtacg gtcagcgagg tttccgcgac gttggtcgcc agcacaatgc
    19741 gccgtccgct gtgcgactgg aacacgcggt tctgttcgct gttcgaaagc cgcgcataaa
    19801 gcggcaagat ttcggtatga cgcagattta gcttgttcag cgcatcggcg gtatcgcgaa
    19861 tttcccgctc accactcata aagatcagaa tgtcgccagg gctttcctga ctcagttcgt
    19921 ctacggcgtc aaaaatcgcc tgcaactgat cgcgctcggt gtcgtcggct tcttcaacaa
    19981 tcggtcgata gcgcacttcc accggatagg tccgaccgga gacttcgata atcggcgcat
    20041 tattaaagtg gcgcgaaaag cgttccgggt cgatagtcgc ggaagtgata atgattttta
    20101 ggtcaggacg ccgcggcagc aactctttca aatagccgag cagaaaatcg atattcaggc
    20161 tgcgttcgtg tgcttcgtca ataatgatgg tgtcgtactg catcagcagg cggtcttgct
    20221 ggatctccgc cagcaggata ccgtcggtca tcagcttgac catcgtgtta tcactaacgt
    20281 gatcgctgaa acgcactttg taaccgatgc aaccgcccgg ctccgttttc agctcttccg
    20341 caatacggtt cgccaccgtt cttgccgcca ggcgacgcgg ctgggtatgg ccgatcagcc
    20401 ctttaatccc gcgccccagc tccatacaga ttttcggtaa ctgagtcgtt ttaccagaac
    20461 ccgtttcccc ggcgacgatc accacctggt gatcacgaat cgcttcgaga atgtcctgtt
    20521 ttttctgact aaccggtaaa ttgtcaggat aagtaatttc cggtcgtgcc gcttcacgca
    20581 gcaggacttt ccctgccgcc tggtcaatct ctttcgccat ctcctggaaa atggcctgtt
    20641 gtgcatcagg atttttaacc ttcttaacac cgtgcagacg ccgagaaaaa cgcagtctgt
    20701 cgcgcagcat cagcgaatcc agccgctgct gcaaggccgt aaaggtcaat ttttgttgtt
    20761 ctgtcataac gttagtgggc agtagtctgc ctgattaatt ttcttttaaa tgatagatat
    20821 agagtaccac atcgcggctt tctatgcgtt gttcaataaa ttcgaacata ggcttcgatt
    20881 tattgcgcta tctctgtggc aggattgtga ataaagtgtc agcaagcaac ggggcatcgc
    20941 ccattcaaac atctataagg aaacaccatg agcaaggtat tagttcttaa atccagcatc
    21001 ctggcagggt actctcagtc taatcagttg tccgattatt ttgttgaaca atggcgcgaa
    21061 aagcactccg ctgatgaaat caccgttcgc gacctggctg caaatccgat tccggtactg
    21121 gatggcgaac tggttggtgc actgcgtccg agcgatgcgc cgctgactcc gcgtcagcag
    21181 gaagctctgg cgctttccga tgagctgatt gccgagctga aagcccacga cgttatcgtt
    21241 attgcggcac cgatgtataa cttcaacatc tcaactcagt tgaaaaacta ttttgacctg
    21301 gttgcacgcg caggcgttac tttccgctat accgagaacg gtccggaagg tctggtaacg
    21361 ggtaaaaaag ccatcgttat taccagccgc ggtgggatcc acaaagatgg accaacggac
    21421 ctggtgacgc cgtatctgtc cacgttcctc gtctttatcg gcattaccga tgtgaaattt
    21481 gtcttcgccg aagggatcgc atacggtccg gaaatggcag cgaaagcgca gtctgacgcg
    21541 aaagcggcca tcgacagcat tgttgctgca taaccggaca cgatcttgat gtggactgta
    21601 gttcagtcca catctcaatc cacttacctg ttttcccata atttcagcat cgctttgtga
    21661 tcttccttaa gcacaatatg cgaacgtctg gctcgaatat aactaatcgc ttcatcaacg
    21721 gttttacagt gtccgtaaca taacaaccat gccgccacta ccagcgcact gcgcgataat
    21781 cccaacgcgc aatggaccag aacgctgcct tgctcttcgc gtaatgtttc cagcatcgcc
    21841 acggcctgtc ggagctcccc ctcttccgga accaccagat ccagcatcgg tacacagaaa
    21901 tagagtcgat cttttgtcgc tcgtccccga gggaattcaa aggtgacgtc cagaaccgca
    21961 ttctgtgccg gaatatgtcg tggaaacgcc cctaaataaa caccagcagt aattttgctc
    22021 accggctcca ggcgacgaca aaaccaacgc atagacagcc acatcccgat gcgccagggc
    22081 aatgtcagcc agtaaacggc gggcgggagt ttcccctgac tatctttccc tgttgttatc
    22141 gcgccaagcc caccgtaccc acggccaata atgagtagcg ataatactgg ccaacataac
    22201 cagactgacc accataactg aatcatcacc attagctcca tcaacacaat gcacgagccc
    22261 gcgcctacga catatggcag tgctattttg attcgacgtt gatcaggttt ctgataattc
    22321 caacgacgat cgacgggcac catccagtca atcaacatac ctaccgccag ccctgtgatg
    22381 acatcaataa aatgatgctg ccaggtcgtc agagtagaaa tagcgatgag taaaaaccat
    22441 ccgccacaga ctttacgcca cctctcagcc agatgctgac gaaagtgacg ccaaagtagc
    22501 cagcagagaa taatatgcag cgaaggagac tggttataag gcagatcaaa cagttcaagt
    22561 tgcgaaaata gccatcccgt caccccactc acttcaggac ggataaaact aaacttcagc
    22621 ggatagagca aaaaaccgca gcaggccatt accgttgcca gaataagccg gtggacaagt
    22681 cggcgctgtt cgaatgtcgt gctacaaacg aacagcgaaa atccatataa aagatccaga
    22741 ctccagtaag gaacaatagt ccagggaaga aaagggatcg ccgtttccca accgaatacc
    22801 tgactgggga tatcatggct gttaaggtcc tgaaccgcgg tgaactgatt aagagatcca
    22861 taggtgaaga agaaaaacgg ggccagcaac aataaccagc cagcgccttg tagcaatacg
    22921 ttccgacgtt cagttatcac gacgcaccgc catcgaaacc gtaaaaatac cccactcatc
    22981 aatccgttgt gtgcatttat caaatccggc atcacgcacg agtgaatcca tctccccttg
    23041 cgaacgcacg cgcattaccc acggtttacc atctttatga ctggttaaca ccccggcaat
    23101 catctccagt tgagggtgcc acggctgccc ggtgtagatg agaatgccgc ccggttcgat
    23161 ggcattggca agacctgcga gtgagttttt tacctgctca ttttcgggaa aaagctcata
    23221 caggccagag acaatcgcca gcgtaggccg cggagttaac gcgctgagtt cctccgggtt
    23281 aaacgcatcg ccctgttcaa aacgcacccg cccagacatt ccccgttggg caatcatcgc
    23341 ttgcccctgt gcaacattta actcactgta atcacgtaac aaaatatcgc ttacggcagg
    23401 ctcgtttgcc agcgcatcca gtacatagcg cccatgccct gcggcaatgt caaccacgcg
    23461 gacggctaaa cctttggcgt ggagatcggc aacggcttgt ttaatcagta tttgtaaatg
    23521 ggttttgcgc tggcgaatat cgcgccagcc aacactgttc aggtagattt tgtcaattaa
    23581 gcgcccgaat gcgttactgc cttgcggttg attttgatag acataatcca gggaactgcc
    23641 tgaatcaaag ccggtgctca ttccgagatg caggcccgca gagtgcgccc cgaacaattt
    23701 catcgccttt cgcataaagc gatacgccaa atcaaccggc gataatggca cgggtccacc
    23761 tgaaaggagc cgccagcgat ccgctgatgg tccagtgcgg tcttcatgct gataatcaaa
    23821 tttttgtgac ttgttagcat ataaacgact aataaagctt tgcatttttt caaatgcctg
    23881 cgccctgttc tcttcaccca acgtgtcgtg ataaaagcct ggcagcagat gcagctcttt
    23941 cagagggcta cgtaatctct gataaaaatc aatctgcggt tggcgatgca ccacatagtc
    24001 atcgcctgat atcagaagtt gcgtggggag cgtaatcgcc gccgcatctc taataatacg
    24061 ttcagacgtt ttgtagagat cgagcaagat attaacggca atcgcccgtg tgatcagcgg
    24121 atcattattg aaactcgcca cccgttgccg atcgtgggtc aaatagcgtc ctttcacata
    24181 ggaattaata aaaaacagac cacgcagacg atgccataac gccagcgcag gacgtgccag
    24241 cggtacatac aatttaacct taaaggccgg agaagccagc accagcccgc gaattgcagg
    24301 cgcataatca tgaacccatg tggcaaccag cactgcgccg acgctttgcg cgatcactac
    24361 cacctcatcc agtccgacct ggctatcgct ggcagcaaaa cgaacaaatt catcgacatc
    24421 ctgcactgaa cgcgcaagag atgggctata accacgcggc cccgaagttt gtccatgccc
    24481 tcgggcatcc caggcataaa aaacagtatc tggcatcgcc agttcatcaa cgagatgttg
    24541 tagacgacca gaatgttcat gaccgcgatg aaataagacg atgacctttt tagccccggg
    24601 ctgtaaagcg ggccagtgcc gataaaacaa cgctgtatta tcactgctgg taaaaaaatg
    24661 ttccccatgg atgcgtgaat tttccatttt ctctattttc cttcagtaac agcagtagcg
    24721 aataaaataa aaaaataccg gagcggtgaa aattaatgag tcaatcctgt caaggagtcc
    24781 gccatgtcct ggtaatagtt tcccactatc tttaacacca atatctcgtt tgatggctga
    24841 catcacgaca tcgccgcaaa aaccactaat accaattaac aaacccgcta ataatgcctg
    24901 taatgtattt agcggtgtca gcaacggtcc aataatcagt gacgcaatca tggtggtgat
    24961 tacgcccccc accagacctt ccagcgtttt tcccgggctg actttgggga ccacttttct
    25021 tctgccgcag gatttccccc ataaatactg tgcaatatcg tttgactcgg ttaatgccaa
    25081 aagaaataac accagtaacg ccccgccctg gatatttgtg gttggcaaca ttaataacca
    25141 ggcggcatga ctcaaggcga aaacggtcat tatccagccc caaaaaatgg cgctcacggt
    25201 atgcagaaaa ccggaggggt cccccataaa aacttgcccg gtggctaata ttagaaaccc
    25261 agccaaaggt ataaataata gatacagctc aaagcaatta aaaccgataa acaaatagtt
    25321 aagaggaata acccaatata accaccgcgg aaaatgtaca gatataagcg tacaatattg
    25381 aaaacttatc agcgcaaaaa acgtcaacgt catccagcgt ggacctgaaa tcaccagtga
    25441 aaaacaaatg acgatgcccc accaggtacg aatacgtaat gtaacttcat cctcttttct
    25501 ctccggtaac cgccatagca gaaaatggtt tatcagcgtt gctaaaatta acaacgcgaa
    25561 aagtgttgcc agagattttt ccagcattat ctttcagcgc tcataagaag cacactccga
    25621 caacgattaa tcgcagtcca gagaagcaga attgaagcaa tgctccataa aagattattc
    25681 cactgcatcc actgtggata aatggcaacg gccagacccc acagaccaaa tattaacgcg
    25741 cgatcgcttt tgccaaatgg accggcataa ctgcgaacac cgttaatcgt ctgcgcgagt
    25801 aaaccgcaaa actcggtcaa tatggtgcaa aataacatga gtatcacgag tgatgcgtta
    25861 ctttccggta aaaataaaaa tggtaaatag agcgcaatat cggaaataac atcaccagtc
    25921 tcattcaaaa ttgccccaag ccgtgtttgc tgattgcact cacgcgccaa catgccatcc
    25981 agcgcattga gcgccatacg gataaaaagc acgatgggca atagcataaa gaggatgggt
    26041 tgtgccacca acatcaacag caatccggta agaagagaaa gcgccagtgc agcaagagtg
    26101 atgtgattcg ctgtaacgtg gtgcttgtaa agccaaaaca tcgtcggcct taacagcgac
    26161 tgaaagagcg gttttatctg gtagagcgtc attatcaatc cttgataatt cggtttgcgc
    26221 tatggtgcat caattcccct gacggagata tcgcaacacg ccgcttcatt aaaaattcag
    26281 aatatatcaa ggtattgggg actcactgtc aggaataatt atccccaatt aatcaaaggg
    26341 ttaagaaata ttacgttgtg gatcaataca actcatcaag ttttctcatc gcttgttccc
    26401 gtattgcgcg cgtcttaccg ttaagcgcca gctggcggac taaatccagt tcagggttaa
    26461 caagctctaa cgccagcaaa ggatcacgtt tcagcctcgc ctttaataca tcaactggaa
    26521 aacggggatt attcatcgcc acaatccacc actcgggatc aatttctgcc gccagtactg
    26581 caggcggtgt gtggcggttg ttaagtgcgc cgaacttgaa aaatgtgaga ttgcttttcg
    26641 ccagtgtact ccaccatata cgatccggtt gttttgccag tacatccagt tgtgctaacg
    26701 catcggggtc gagtgcataa tagacgcgta atgcttcatc aacctgcgca cgggagctgt
    26761 tttcctgcct cagaaaaaac ttcgcccaaa tatcattgcg tggcgtattt tgctgtaatt
    26821 ccttgaggac aacagcgggt aaattatccc aactaactaa tgcactctct aaaaagtccc
    26881 agtcttccaa acgtatcagg gcggtggcat ttaatagcat ctgatcatca acttcaccgt
    26941 ggctgataag ctcagacaaa atcatatcag cgctggtttc atacgcagct gaatctgctt
    27001 tcggattatt tttccgatgc gataaagcac gttgccaaat tttttgtttc atcgacgtgc
    27061 tcagatactg atttttcgcc agattactcc acccctcagg atcgttgaaa cgtgtcaata
    27121 accgttgtag agcagcgtca gagtgaatta cctgcatatc ccgacagcaa aagtaggcag
    27181 gggatttttt ctccatcctt ttaacaaaat taaactgtaa gttatctggc aatgctaaaa
    27241 ataacacacg tattagatca tcgtcattct cgaatttttg gtataaatag gctgcaactt
    27301 tgtcactctc ttgtggcgtc atccgcaata attgaccatg gtgtttcatc tctgccagct
    27361 tgaacgctaa gcttgtgaca acattgtttg atgaatcgtt cgccaactgc attgcctgat
    27421 tgtaggtgag ttgtcctcca gcaccgacct gacgaataga ggaatcatca ctgcggatat
    27481 agccatcaat aatggattca ggcaataacc acgcatcttt ataaagctcc tgaatgatca
    27541 tatcccaatc tcttttcgcc agagtcatta cgatatccag agggagattt tcacgtagcg
    27601 caagattcaa cggaccgaca ccatcaggta gacgactcat tcgcgtcatt atttcgacag
    27661 agagcggttc acggtcgagc aaccccagaa caacaggctg gtaacccaat tgctgatcgg
    27721 tcaactccag aatctcattg tcaggcatat atctcgccat actttcaatc gtttgttcat
    27781 cattgctatt aataagttgt ttgcgtaggt tagctgaaat tcctttaagc cttgggagag
    27841 ttcgcattat ggcaagatca aatgtcgcaa tatctcgtcc ttcaaatgca gcaacgttgc
    27901 cttcatccag catttgcaat tcctgcacta actcaggaga aggcttaggg cgaaacagcg
    27961 cttctttttt catctgatta cagacattcc ctgccatgcg aatattgctt cgcttaaaaa
    28021 tttctttcag agcatcttca tcatttatta gaccccaata atatgcatca ggatttatta
    28081 tcatcagcgc ttttttgaca ttttcagtgt catttttgtc actcgcacgg accatgaatt
    28141 cactatttgt cgaagacgca acaacaggtg caattatgtc attgataaat attccacgcg
    28201 cattaatact ccccttaatc gaagacggat tatcgtaata tgagtcgata aataccagtc
    28261 cacttgcatt tatcgaaccg gtaattaaca gtggtgaatc cgtaactata ttcgttgccg
    28321 tcacgttacc gagaataacc catggattag aaagagagaa agtggaaata ttacctttga
    28381 ctgtcaaatt accaagaaca atcaattttt gattactctc tatattacct tctaccacta
    28441 aatcgccacg atgcactttc cagtccgatg cagataaacc gaaggggaca agattcacgc
    28501 tactgttgat agcttgttca acttgttgtt tatcttcagc ggtagccgca gaaaaaaatg
    28561 gttcaatatc aactgtattt gcaacacatc gaagttccgc ctgtactgga agactcagca
    28621 taaacagggg aagtaaacct atccagccgc aaatcctgga cttaaacata tgcactccat
    28681 gccattaaaa aagaaacaaa agggaaatgc atctttccct tttgtatatt cgttattctc
    28741 gcgaaatacc atccaacgta gacaacactt cctcagaaag atgcaatttt tcagcagcca
    28801 tattctcccg taaatgcgca acagaagacg tccctgggat cagcaaaata ttcggtgaac
    28861 gttgtaacag ccacgccagc gccacctgca tgggtgttgc acccaggctc gcagcaacat
    28921 ccgacagcgt agaggattgc agcggtgtaa agcccccgag cgggaagaac ggcacgtagg
    28981 caatgccgtc acgagccaaa gcatcaatca ttgcgtcatc agcacgatga gcaatgttgt
    29041 attcgttctg cacacagacg atttcggcaa tcttgcgcgc ctctgcaacc tgcgtcggtg
    29101 tgacgttgct taggccaata tgttttacca ggccttgttg ttgcatctct gccagcacgg
    29161 tcaggctggc ttcaatcgat ccttccgctg ggccatgacc atcccccatc ataatgcgca
    29221 agttaaccac atccagtacg tccagcccga gattacgtag attatcgtgc accgcttttt
    29281 gcagctctgc cggagaaaat gcgggcaacc aggatgcgtc ctctccacgc cgcgcaccaa
    29341 ttttagtgac aattgtcagg tcgtcagagt aaggataaag cgcttcgcgg ataatctgat
    29401 tggtgacgtg cggaccataa aagtcgctgg tatcaatgtg attgacgccc aatgccagcg
    29461 cctcgcgcag cacggttata gcgacgtggc gatctcttgg gggaccaaaa accccaggac
    29521 ctgccagttg catcgcgcca taaccaagac ggttaacgga ttttgtaccg agagtaaatg
    29581 tattgctgct catcgcaccc tgccctgtaa taaaacggat tgagaaaaat cagtatagaa
    29641 acgaaaagag ataaataatg cgaaggcgta aacaagatta ctggatgagt tgctctattg
    29701 tcctgaaaga caccaaaaca aaacgcgtaa agcgccgcat tcggcaataa aaaaaccgca
    29761 acctgtatca gcgttgcggt ttaggtatca catgctaaaa attagaacgt tcagtatctg
    29821 gacgttactg tttcggtcgc agattctcat caaataccag gcgtttcctg ccaggttcaa
    29881 cttcccgcaa tggttcgact tgatgtaacg tctgcttaca gcgtgtcacc agcacctgat
    29941 actcatgcgt accctgcttt ttccatgcca gctcctgctc actaagctca cgaatcagtt
    30001 tcgccgggct gccgacaatc aggtaattag caggcatttc tgctttggct ttcacaaatg
    30061 cggatgcgcc aacaatgctg ttctcgccaa tcaccgcacc atccatcact accgcgttca
    30121 ttcccactaa tgcattgcgg cggataatgc agccgtgaag gatagcgcta tgaccaatat
    30181 gcccatcttc ttcaacgaca gtatcctgct cgggaaaacc gtgcataacg caattatcct
    30241 gaatgttcgc gccatctttc accacgatac gaccaaaatc gccacgcagg ctggcatttg
    30301 gcccaacgta aacacccttg ccgagaataa catcgccgat caataccgct gtcggatgga
    30361 caaaactctc ttctggcaca accggagtca gaccgtctat ctgataaatt ggcataacgc
    30421 ctcctgttca atattcaaac cgccaaaacg ttgaaaatac aggctacccg gcgaaggcag
    30481 ttcaccgacc gaggtttcac ctttttcact aacgaacgct aaagcagcag gcgctacgcg
    30541 ctgataaatg ttgatacaca gctgacgcgc cgtatgccct gcccagtgag ccggaagcaa
    30601 ctcctccggc aacaaagggt ctttaaggac gacacggcga taaaaatgga tcagtaaaag
    30661 ctgaatatga aatgcacgct ccggggttaa ctcgtctgct gcctctttta acagctgcac
    30721 caacgggcgg aatgactgaa taaaggtttc gtacatggca ttttgttcag ttaaatgcca
    30781 gcactcttct actctggcac gcagtgctgc acgagaaagc gccagtggta tttgcgcttc
    30841 aaaacaaatc acgttatccg ccacacccgc ttcatgcaaa agtgtctgta catcggccag
    30901 tttttgcgac ggcgatgcca tcaggctggg tgccagtgcg ccaaaacctt gccagatcaa
    30961 ctgctttttg acatcagcca gcgttgattt atctaaacct tccgaaagca acaggagcca
    31021 tttaccatcc catgcaggtt gctctgcgcg ataaatttta ctttctgccc gtcgcgtcag
    31081 gcgcaagcct ttatcactga ggctatagaa actgcgtcgc ccgatgcggg aaacatccag
    31141 ccagccttct ttattaagac gaaacaaagc ggtgcgcacg aatcgctcac caaatcccag
    31201 cccttccagt aaagcagcca gactacccag ccagatttca ccaccacgat gggaaagcga
    31261 atcaccatac agagaggaga tcaaagatgt gccactgaca ggaacagcgt ttacagcatg
    31321 ttggataaaa gtatcaagtt tactcattcg attcagtctg ttgttattgt tgtgatttat
    31381 gaatcatagc acaggtcgta tcgacttctg gccctggcgc tttaccccac ccgggccaga
    31441 cgacacgtca ggcaccaaca atattgcgca gatcaaacac ccgacacgcc ttgccttctg
    31501 aacgcgggat actgccacag ttaacgatca tcacatcggt agagatcccc accatcgact
    31561 taatccgatg gcgcagctgg tggcacacct ggcaacgctg ctcatgcgtc aatgtcaggc
    31621 tgctttcttt caactccact ttcacagaaa gtgaatcaag atgcccccgg cggttcacct
    31681 ccagttggta atggggcgat aaatgttcga atttgacaat ctcttcttcc agttgtgacg
    31741 ggaagacatt tacaccgcga atgatcaaca tgtcatcgct gcgtccgctg atgcgatcca
    31801 tgcggcgcat agtccgcgcc gttcctggta acagtcgggt taaatcacgc gtgcggtaac
    31861 gaatgaccgg caatgcttct ttggtcagcg tggtgaataa cagttcgcca tgttcaccat
    31921 cggcaagcgg tgtgccgtca tgaggattaa caatttcagg atagaaatga tcttcccaaa
    31981 tggttgggcc gtcggtagtt tccagacact ccatcgccac ccccggcccc atcacttctg
    32041 acaggccata aatatccagt gcggtgatcc ccagacgccg ttcaatctct ttacgcatgg
    32101 cctgtgtcca cggctcagca ccaaacactc caacccgcag cgagcaaccg ctggcatcac
    32161 cgcccaactg ccgctccagc tcttcaatca ggttaaggca ataagatggc gtaaccatga
    32221 tcatatctgg ttgaaaatcg cggatcagtt gcgcttgttt ttccgtctgc ccaccagaca
    32281 tcgggatcac cgtagcgccc agacgttcgg caccatagtg cgcgcccagc ccaccagtaa
    32341 acaggccgta accataggca acatgaattt tgtctttggg cgtgccacct gccgcacgca
    32401 aggaacgcgc tacaatattg gcccagttat caatatcgtt ttgcgtatag ccgacaactg
    32461 tcggtttacc tgtggttccc gaagaagcat gaatgcgcac cacttgttcc atcggcacgg
    32521 caaaggtgtc gaagggatag ttgtcgcgca gatcctgttt ggtggtacag ggaaatttac
    32581 gcagatctga aagttccctg aaatcatcag gatgtacgcc tgctgcgtca aatttgcgcc
    32641 gatacatcgg gacattttca tacgcgtgtt tgagcgtcca tttcagacgt tgtgtttgca
    32701 acgcctgtaa ctcatcaaca gacgcggttt caatcgggtc aagctttgta ttggttatca
    32761 ttgtagggta ctcgcaggtt gatatgctca aacacgctcc agaatcatgg cgatgccctg
    32821 accgacaccg atgcacatgg tgcacaatgc gtaacgaccg ttacgccgat gcagctcatg
    32881 gctggcagcc agtgccaggc gggcaccact cattcccaac ggatggccta aggcgatagc
    32941 gcctccgttg gggttaacat gtggggcatc atcaggcagc cccaattcgc gtagtacacc
    33001 caacgcctgg gccgcgaacg cttcgttcag ttcaatcacg tccatatcgt gaatactcag
    33061 ccccgcgcgt tccagcaccc ggcgagttgc aggcaccgga ccaagcccca tcaggcgcgg
    33121 ttccaccccg gcggttgcca tggctacgat tcgcgcccgc ggtgtcagtc cttgcgctgc
    33181 tgccatctgt tcactggcaa taatcaacgc agcggctccg tcattcaccc cggaggcatt
    33241 gcctgcggta atcaccccat tggcacgaaa tggtgctttt aacccacgta actgttccag
    33301 cgtcgtttcc gggcgcagat gctcatcatg ttgtatttct gttacaacgc ctttcttgtt
    33361 tttcaacaca accggaacaa tctcctcagc cagaatgcct gaggattgcg cttttgccgt
    33421 acgttgctga ctgcgtagcg caaaactatc ttgatcttct cgcgatattt ttaacagttc
    33481 agctacattc tctgccgttt ccggcatgct gtcagttcca aattgttgag ccatgagcgg
    33541 gttcacaaat cgccagccaa tagtggtatc aaacatctca gcctgacgag aaaatgcact
    33601 ggttgccttg cccataacaa acggtgcccg agacattgac tccacgccac cagcgatcag
    33661 caaatcgcca tcgcccgctt taatcgcccg tgcggcgaac cccagtgcgt ccagcccgga
    33721 accacacaag cggttaatgg ttgtgccgga aacactctgc ggcagccccg ccagtaacgt
    33781 cgccatccgg gctacgttac ggttgtcttc tcccgcctga ttagcacagc cgaggatcac
    33841 atcatcgata cactccgcat cgagacgcgg gtttcgcacc agcagctccc gcaaagggat
    33901 agcagccaga tcgtcagccc gaacacctga taatgcccca ccatagcggc caattggcgt
    33961 acgaattccg tcacagataa aggcttcacg catcaggctt ctcctgtaat ggtgccgccg
    34021 atgcggtgag atttaccgcg aaacagcgca accgtttttt gttgttggtt aacaatttcg
    34081 atgtcgtaaa caccggtttg cttgccctga tgacgtacct gcgcagtagc agttaaggtg
    34141 tctccggcaa agcctggacg taaaaaatca atcgtgcagg cagaagcgac ggctgccagc
    34201 ccctggctat tgcaggcgta ggcaaaggca gtatcagcca gtgaaaatag ctgcccgccg
    34261 tggcaacttt gatgaccgtt aagcatttgt gcagtgacgg tcatggtcac tacagcaaag
    34321 ccttcatcca ttgagataat gtcgatgcca agcgctttgg cgcaggcatc gttctcatac
    34381 attgcatggg cattttgcca ggccttatga ctcataaccg ctctccagaa gcgcccgttg
    34441 gcgcagcaat gaacatgggc gatagcgttc ttcgccatag tgatgctgta gattttcaag
    34501 gagccttaat attcgctgcc agccaagttg cgctccccag gcaagtgggc catatggata
    34561 attcacccca agacgcatgg cggtatcgat atcctgttca gaggccacgc ctttttgaag
    34621 cgcatcaagg gcttcattga tgatcattgc taccgttcgc caaatcagca ttcctgggta
    34681 atctgcaatt tgcagcactg ttttgccctg ctgttgcagg taataaatgg ccttgcgggt
    34741 cgctgagtcc gggttcaccg ctgccgcagc aatggtcacc accttgcccg ccattttgtc
    34801 gaccactacc accgggcgtg ccagtcgtat tgccagcgcc tgtgccgtct cgccttgtgt
    34861 ctcaatcaat aaaacatcgt caatttccgt gacaccgtca cttttctttt ctactttcat
    34921 tgggctaaaa ctgtcgctta ccgcttccag gccaacaact gcctcacgtt ccgcgcgcca
    34981 gtcgtacacg cccagcccac ttttcttgcc caaccgtcca ccaatcacca gttcctgttg
    35041 caccagcgaa ggtaaaaaac gacgctcctg ccagaaagcg ttaaacaccg aacaggtgac
    35101 agcaaaattg acgtcctgac caatcagatc ggttaattcc agcggcccca tcgggaaacc
    35161 agcgccatcg cgaagtgcag cgtcaatcac ttctggtgca gcaacctgct cttccagtgc
    35221 ccgccaggcc tcggaataat aaggacgcgc aacacggtta acgataaatc cgggggtcga
    35281 atgacagcgc actggctgct taccccaact caatgtcagt tcacacaact gctcgacaac
    35341 ttccgctgcc gttgccagcc cactgaccac ctccaccaac ttcatcaccg gtgccgggtt
    35401 aaaaaaatgc agccccgcaa cacgttcagg attttttacc tccgcagcaa tcgcggttat
    35461 agagattgac gaagtgttag tggtcaatag cgtttgtggc gggcaaactt ccgccagttg
    35521 tgcaaagagc gcttttttaa cttccagacg ttcagacgcc gcttcaataa ccagattcgc
    35581 tgctgccagc gcgtgaatat cggtcaccgg gatcaggcgt ttcaatgtgc gttcacaggt
    35641 ttcagcggtc agttttcccc gcgtcacgcg tgaatttagc cgcgcgtgta tcccgtcgat
    35701 tgcgcgggtc agcgcttcag cagaaatgtc atacagtaaa acctgatgtc catgactggc
    35761 ggcaacttca gcaatgcctg cccccatggt gccgctccca atcactgcca cagtttgcac
    35821 atttatcatc atgctatttc cccgtgaact gcggtgagcg tttagccagg aacgcactga
    35881 caccttcacg ataatctgcg ctccgtccgg caagtcgctg atagtcacgt tccagatcca
    35941 gttgcgtatc gagcgtattg gtttcagcgc tatttatcgc ttgcttgata agtccaagac
    36001 caaatgtcgg ttgtgtcgcc agatgccgtg ccagctgttg cgcggtatct gccagcgttt
    36061 catcatcaac aacctgccag atcatccccc attcgtgcgc ctgttcagca ctcagttgat
    36121 tccccagcag tgccagcccc atggcgcgcg ctcgtccggc aacgcgtggc agtaaccagg
    36181 ttccaccgca atcgggtatt aagcctaact tactaaacgc catgacgaat tttgctgaac
    36241 gggcagcaat aacgatgtcg caccccagtg ccagtgttgc gcctgcccct gccgccacgc
    36301 cattgactgc acagatcacc ggttttggca gttttgccag gcgacgtacc agtgggttat
    36361 agaaacgttc aactgacatc cctaaatccg gtgcggggcc tgtgggatca acgttacgat
    36421 cgttaagatc ctgaccagca caaaatccgc gcccggcacc tgtaagtaac aggcaacgga
    36481 tagtgtcgtc gcgctcgacc tgtttcaggc actctgccag ttgtgcgtgc atctcatcat
    36541 taaaactgtt caggcgttcc gggcggttga gcgttagtgt catcacgccc ttttctacat
    36601 gactgaggat gaattccatc attagcgtcc tttaaagtcg ggcgtgcgtt tttgtaagaa
    36661 agcggagatg ccttcatgac gatcttctgt tgccgccagc aaggtgaata actgtcgctc
    36721 ctgggcaagt ccggcttgca aagccacttc ctgcgactgg cgcagcgctt gctttgccgc
    36781 ttgtaaggcc agcggcgagt gacgtgccat tttcgatgcc agctgtaagg cgtattcgag
    36841 ggttaaatcg ctggggaaga cgtcgctaac cagcccggcc tgctgtgctt gctgggcggt
    36901 gatactttct ccgctcagca ccattttgct ggctaacgat ttaccgacac tacggattaa
    36961 acgttgcgtt ccgcctgcgc caggcatgat gccgagagtg atttccggca acccaaaacg
    37021 cgcgttctct ccggcaacca ccacatcgca caacaatgcc agttcgcaac ccgccccaag
    37081 cgcgtaacca ttgacggctg cgataagtgg tttattaaag gcctgcaatc gcgcccatag
    37141 ctgcggacgt gtatcgttta aggtggccgc gagatctttt tctgccattt cgttgagatc
    37201 ggccccagcg gcaaaaaagc gtgcattacc ggtaatcaca cagaccgaaa tgctggtatc
    37261 ggtagccgca gcttccagtt catttaccag ttgcatcagc agggcattat ttagcgcatt
    37321 acgtgcggcg ggacggttaa gggtcagcaa caatactcgt tgctgacggc tgacgatcag
    37381 ttcgctcatg ccatcccctt cgcgtcaaag tcaaccacca catcactggt cagcggcagt
    37441 gcctggcaac tcaacacata gcctgcagcc agttcatccg gttccagact gtaattggtt
    37501 tccatcgcca ctttgccacg cagcactttg catttgcagg tcgcacagac gccgcctttg
    37561 caggcatagg gcagatcagc cccctggcgc aatgccgcat cgagaatgct ttcatcgtcg
    37621 gcattaagca cgatttcccg atcccgccca tcctgacgta cagtcacttt ttgtccgtca
    37681 ctttgcacgt taacgctacg tttgacgcgc gtgccaggcg tattaaaccg ctccagatga
    37741 atggttttat ctggcattcc cagtgctttt aaggcggctt ccgtctcatc catcatcgcc
    37801 gccggaccac aaataaatgc ctcatcataa agacgaaaat taatgagcga ggccccaagt
    37861 gactgtaatt tttcaccgtc aatacgcccg tgaagcagat cgctgtcgag ggtttcctga
    37921 ctgaaaatgc acaacaactg taaacgctga ggatatttgt ctttcaggtc tgccagtgcc
    37981 tggcgaaaca tcatgctctg gctggtacgg ttaccgtaga tcagggtgaa ctgactttca
    38041 ggctcggttt gtaaagtggc ggcgataatc gccagcattg gcgtaatacc tgatcctgct
    38101 gcaatagcca gatagcgccc ctggcgttcg gcctgcggct gatagccgaa atggccctgc
    38161 ggcaccatga cttccagcgt catcccctga cggatgtgat cgcgggcata gcgggagaaa
    38221 cgtccgcctt caatggcttt taccgccaca ctaatttcgc caggcaggta gctgcggcaa
    38281 atggagtaac aacggcgtaa ttcttcacca tcaaggctgg cttttaaggt caagtgttga
    38341 ccggggcgaa agcgatacgc ctcctgcaaa ggctggggca ccgcaaaggt aatggtcacc
    38401 gcatcacggg tttccggctc cacttttgcc accgttaagg aatgaaacgt tgtcatggca
    38461 tcctcaaata catttgaaat aatcgaaagg ttcgcggcaa ctatcgcagc gatacaatgc
    38521 tttgcaggcc gtggaaccaa attcactgat aagtgtggta tggacgctgg cacagcgcgg
    38581 gcaacatact tctggcggca aatgggcatg gcaactgtgt ccggcgggcg ggctaatgcc
    38641 atactgtcgc agacgctcac gggcatcggg ggtcatccag tcggtggtcc atgccgggtc
    38701 aagttgcagc acaacctgaa cgggggtaaa cccgtgggtt gacattgcct cacgtatcgc
    38761 gccaatcaaa tgttccgttg ccggacaacc ggaatatgtc ggcgtaaagc cgatcaccca
    38821 tccttctccc atctgtgtca cattacgcac catgcctaaa tcagtaatgg tcagcaccgg
    38881 gatctccggg tccgggatct ggctgagtag cgcccatatc tcatgaacct gtggcggtgc
    38941 aatggtggcc agacgttgca tacccatctc ctctgttacc attgctgacc gggcaagaca
    39001 cgctggagat actgcatttc tgccagcatg ggtccaaggt gttcggtatg cagtcctttt
    39061 ttaccgccag tgcgatacgc ctgctcttgc ggtacgttca atgtggcttc gttgatccca
    39121 gcaaaaactt cggcttccca cgctgcgcgt aaagtgcgtg gatcaaccgc aataccctct
    39181 tcactcagtg caatatcaat ctcgtcggca tcgaacagtt cggcggtaaa acgccacaac
    39241 ttgttgatgg cctgctgcat cttttgccct gatacgtcag taccattgcc cagtcgctcc
    39301 agccagccac gactaaaacg caggtgataa cgcgcttctt taattgcttt ggcagaaatc
    39361 gccgccagtt gcggatcacg gctctccatc agacgggtaa agagcgccac atgccatgca
    39421 tcgatgaaat actggcgtgc aatggtgtcg gcaaaattgc cgtttggctg ttcaaccaac
    39481 aataagttgc tgaactggcg ctcgtctcgg gtgaaggcca gggtatcttc atcgccttct
    39541 cccgctaatt cagcggcata cgataagaag ttgcgtgcct ggcctaataa atcgagtcca
    39601 atatttgcca gtgcgagatc gatttccagt tccggtgcgt gaccgcacca ttcccccaac
    39661 cgctgggaga gcaccagaca gttatcgccc aggcgcaagg tgtaagccgt taactgattc
    39721 atttccgacc tcacatgtgc tcaatgccat cagggatggt gtaaaacgtt ggatggcgat
    39781 agaccttgct ttcagccgga tcaaaaaatt caccgcgttc ttccggttgc gaggcaacaa
    39841 tttcactcgc cttcaccacc caaattgaac atccttcgct acgacgggtg taagcatcac
    39901 gggcattttc cagtgccatc cgctcatcgg cagcatgtaa actgccaaca tggcggtgtg
    39961 ataagccctg tttgccacgc acgaaaactt cgtataacgg ccagtaaaca ttactcattt
    40021 tgaatctcct tatgcgacct tacgggcaag ttgtttttgt gcatgggcca gcgctgcttc
    40081 ccgtacccag gtaccttctt cccaggcttt acgtttggcg tcgagcctct cctgattaca
    40141 aattccgcga ccgttaatta cttcgttaaa ctcctgccag tcgatctcac caaagcggta
    40201 gtgaccgctt tcagtgtcaa aatgcagatc cgggtcagga acggtcatac cgagcatttc
    40261 aacctgtgga acggtgttgt ccacgaaacg ctggcggagt tcgtcgttgg tgaaacgttt
    40321 gattttccag gcgagacttc tggcgctgtt tggcgagtta tcatcgtttg gcccgaacat
    40381 cattaaggct ggccaccaga aacggttaat ggcgtcttgc aacatctgct tttgagcttc
    40441 gctgccctgc gccagtgcca tgcaggcttc aaacccctga cgctggtgaa aactctcttc
    40501 tttgcagatt ttcaccattg ctctggcata cgggccataa gaagtgcggc ataacgccac
    40561 ctgattgacg atcgctgcgc catccaccag ccagccgata acaccgatat cagcccaact
    40621 caatgtcgga taattaaaga tggaggaata tttcatccgt ccgtcgagca ttttttggta
    40681 gatgtcttct ctggcgcagc ccagtgtttc ggcggcgcta tagagataca gaccatgacc
    40741 ggcttcatcc tgcactttgg ccaacagaat ggctttacgc cgcaaggttg gtgcgcgagt
    40801 gatccagtta ccttcaggca acataccaac aatctcggag tgcgcatgct gcccaatctg
    40861 gcggatcagt gtctttcggt aagcatcggg catccagtcc tgcggctcga tagccgtttc
    40921 ctgggctatc cgttgctcaa agcgttcttc ttgggtcacg tcatcacctt tacgattcca
    40981 gttttcgtga attaattaaa aacacgaatc acgttgtttt atgaaagtta cacaatggtt
    41041 aactaaaatc acaaattttg tatgtttgaa ctgtgacgga tttcgcaccc tatttttata
    41101 actcattata tatcagtgca ttaatgtttt tagtggttga tatacagatc gcatagttaa
    41161 catttcgtta aaatacgcct tgctttttat gattcgtgat ttaactatta gcaacagaaa
    41221 tgtgaaacat ctggagagta gcgatgcagc agttagccag tttcttatcc ggtacctggc
    41281 agtctggccg gggccgtagc cgtttgattc atcacgctat tagcggcgag gcgttatggg
    41341 aagtgaccag tgaaggtctt gatatggcgg ctgcccgcct gtttgccatt gaaaaaggtg
    41401 ccccagccct tcgcgctatg acctttatcg aacgtgcggc gatgcttaaa gcggtcgcta
    41461 aacatctgct gagtgaaaaa gagcgcttct atgctctttc tgcgcaaaca ggcgcaacgc
    41521 gggcagacag ttgggttgat attgaaggtg gcattgggac gttatttact tacgccagcc
    41581 tcggtagccg ggagctgcct gacgatacgc tgtggccgga agatgaattg atccccttat
    41641 cgaaagaagg tggatttgcc gcgcgccatg tactgacctc aaagtcaggc gtggcagtgc
    41701 atattaacgc ctttaacttc ccctgctggg gaatgctgga aaagctggca ccaacgtggc
    41761 tgggcggaat gccagccatc atcaaaccag ctaccgcgac ggcccaactg actcaggcga
    41821 tggtgaaatc aattgtcgat agtggtcttg ttcccgaagg cgcaattagt ctgatctgcg
    41881 gtagtgctgg cgacttgttg gatcatctgg acagccagga tgtggtgact ttcacggggt
    41941 cagcggcgac cggacagatg ctgcgagttc agccaaatat cgtcgccaaa tctatcccct
    42001 tcactatgga agctgattcc ctgaactgct gcgtactggg cgaagatgtc accccggatc
    42061 aaccggagtt tgcgctgttt attcgtgaag ttgtgcgtga gatgaccaca aaagccgggc
    42121 aaaaatgtac ggcaatccgg cggattattg tgccgcaggc attggttaat gctgtcagtg
    42181 atgctctggt tgcgcgatta cagaaagtcg tggtcggtga tcctgctcag gaaggcgtga
    42241 aaatgggcgc actggtaaat gctgagcagc gtgccgatgt gcaggaaaaa gtgaacatat
    42301 tgctggctac aggatgcgag attcgcctcg gtggtcaggc ggatttatct gctgcgggtg
    42361 ccttcttccc gccaacctta ttgtactgtc cgcagccgga tgaagcaccg gcggtacatg
    42421 caacagaagc ctttggccct gtcgcaacgc tgatgccagc acaaaaccag cgacatgctc
    42481 tgcaactggc ttgtgcaggc ggcggtagcc ttgcgggaac gctggtgacg gctgatccgc
    42541 aaattgcgcg tcagtttatt gcagacgcgg cacgtacgca tgggcgaatt cagatcctca
    42601 atgaagagtc ggcaaaagaa tccaccgggc atggctcccc actgccacaa ctggtacatg
    42661 gtgggcctgg tcgcgcagga ggcggtgaag aattaggtgg tttacgagcg gtgaaacatt
    42721 acatgcagcg aaccgctgtt cagggtagtc cgacgatgct tgccgctatc agtaaacagt
    42781 gggtgcgcgg tgcgaaagtc gaagaagatc gtattcatcc gttccgcaaa tattttgagg
    42841 agctgcaacc aggcgacagc ctgttgactc cccgccgcac aatgacagag gccgatattg
    42901 ttaactttgc ttgcctcagc ggcgatcatt tctatgcaca tatggataaa attgctgctg
    42961 ccgaatctat ttttggtgag cgtgtggtac atggatattt tgtgctttct gcggctgcgg
    43021 gtctgtttgt cgatgccggt gtcggtccgg tcatagctaa ctacgggctg gaaaacttgc
    43081 gttttatcga acccgtaaaa ccaggcgata ccatccaggt gcgtctcacc tgtaagcgca
    43141 agacgctgaa aaaacagcgt agcgcagaag aaaaaccaac aggtgtggtg gaatgggcgg
    43201 tagaggtatt caatcagcat caaaccccgg tggcgctgta ttcaattctg acgctggtgg
    43261 ccaggcagca cggtgatttt gtcgattaat cggtgaatga agggcaacgg cgaatagttg
    43321 cccttttatt tcactaaatt ttgtgacgtt gtcacattat gcatgatgtg tacatctatt
    43381 ttcagggcat ccactgtatg aaaagctggc acacctgcca aacaacctgg caggtgcagg
    43441 caatcccctt tgcatcagta ctgataatgt gaacctgact aaaccgccca cagagcgcgg
    43501 ttgctaacaa gaacacaaca tctgacgagg ttaataatgg gaagcccctc tctgtattct
    43561 gcccgtaaaa caaccctggc gttggcagtc gccttaagtt tcgcctggca agcgccggta
    43621 tttgcccacg gtggtgaagc gcatatggtg ccaatggata aaacgcttaa agaatttggt
    43681 gccgatgtgc agtgggacga ctacgcccag ctctttaccc tgattaaaga tggcgcgtac
    43741 gtgaaagtga agcctggtgc gcaaacagca attgttaatg gtcagcctct ggcactgcaa
    43801 gtaccggtag tgatgaaaga caataaagcc tgggtttctg acacctttat taacgatgtt
    43861 ttccagtccg ggctggatca aacctttcag gtagaaaagc gccctcaccc tcttaatgcg
    43921 ctaactgcgg acgaaattaa acaggccgtt gaaattgtta aagcttccgc ggacttcaaa
    43981 cccaataccc gttttactga gatctccctg ctaccgccag ataaagaagc tgtctgggcg
    44041 tttgcgctgg aaaacaaacc ggttgaccag ccgcgcaaag ccgacgtcat tatgctcgac
    44101 ggcaaacata tcatcgaagc ggtggtggat ctgcaaaaca acaaactgct ctcctggcaa
    44161 cccattaaag acgcccacgg tatggtgttg ctggatgatt tcgccagtgt gcagaacatt
    44221 attaacaaca gtgaagaatt tgccgctgcc gtgaagaaac gcggtattac tgatgcgaaa
    44281 aaagtgatta ccacgccgct gaccgtaggt tatttcgatg gtaaagatgg cctgaaacaa
    44341 gatgcccggt tgctcaaagt catcagctat cttgatgtcg gtgatggcaa ctactgggca
    44401 catcccatcg aaaacctggt ggcggtcgtt gatttagaac agaaaaaaat cgttaagatt
    44461 gaagaaggtc cggtagttcc ggtgccaatg accgcacgcc catttgatag ccgtgaccgc
    44521 gttgctccgg cagttaagcc tatgcaaatc attgaggctg aaggtaaaaa ttacaccatt
    44581 actggcgata tgattcactg gcggaactgg gattttcacc tcagcatgaa ctctcgcgtc
    44641 gggccgatga tctccaccgt gacttataac gacaatggca ccaaacgcaa agtcatgtac
    44701 gaaggttctc tcggcggcat gattgtgcct tacggtgatc ctgatattgg ctggtacttt
    44761 aaagcgtatc tggactctgg tgactacggt atgggcacgc taacctcacc aattgctcgt
    44821 ggtaaagatg ccccgtctaa cgcagtgctc cttaatgaaa ccatcgccga ctacactggc
    44881 gtgccgatgg agatccctcg cgctatcgcg gtatttgaac gttatgccgg gccggagtat
    44941 aagcatcagg aaatgggcca gcccaacgtc agtaccgaac gccgggagtt agtggtgcgc
    45001 tggatcagta cagtgggtaa ctatgactac atttttgact ggatcttcca tgaaaacggc
    45061 actattggca ttgatgccgg tgctacgggc atcgaagcgg tgaaaggtgt taaagcgaaa
    45121 accatgcacg atgagacggc gaaagatgac acgcgctacg gcacgcttat cgatcacaat
    45181 atcgtgggta ctacacacca acatatttat aatttccgcc tcgatctgga tgtagatggc
    45241 gagaataaca gcctggtggc gatggaccca gtggtaaaac cgaatactgc cggtggccca
    45301 cgcaccagta ccatgcaagt taatcagtac aacatcggca atgaacagga tgccgcgcag
    45361 aagtttgatc cgggcacgat tcgtctgttg agtaacccga acaaagagaa ccgcatgggc
    45421 aatccagttt cctatcaaat tattccttat gcaggtggta ctcacccggt agcaaaaggt
    45481 gcccagttcg cgccggacga gtggatctat catcgtttaa gctttatgga caagcagctt
    45541 tgggtaacgc gttatcatcc tggcgagcgt ttcccggaag gcaaatatcc gaaccgttct
    45601 actcatgaca ccggtcttgg acaatacagt aaagataacg agtcgctgga caacaccgac
    45661 gccgttgtct ggatgaccac cggcaccaca catgtggccc gcgccgaaga gtggccgatt
    45721 atgccgaccg aatgggtaca tactctgctt aaaccatgga acttctttga cgaaacgcca
    45781 acgctagggg cgctgaagaa agataagtga ttgtttcaga caaaaaaacg gcaccaggtg
    45841 ccgtttttta cttatgagcg aaccagatta ataccgtaca cacaccgact tagtttcaca
    45901 ccaaccgtcc agccagtcgg ggccaaaatc acggcccgtt cctgactgct tcatcccacc
    45961 aaacggtaag ttagcgtcaa ttaaggtatg gctgtttacc cacaccgtcc ctgcctgtaa
    46021 gcgatcgcta tattccagag cctgggagag attttgcgtc cagacactgg cagttaagcc
    46081 atattccgtg tcgtttgcca gttgtaacgc ctcttctcca tccgctactc gcaccaggtt
    46141 taccaccgga ccaaacacct cttcacgagt taagcgcaat ttagcatcgg gatttaccac
    46201 cagcgttggc gcaacataat acccctctcc ggctggtcca ttcgacccgc gaatcagctc
    46261 tgcttgctgt gcctgcgcat cgtcgaggaa tgaacacact ttgtcgcagt gcgcacgaga
    46321 aaccaaaggg ttaatctgtg caacaggtga catccccggt cccacttgca acgattttac
    46381 cgcctgctca aatccactaa ccagcgtgtc aaacaacggc gcttcaatat aaattcgcga
    46441 actggcggcg catacttgcc cttgattcag gaagcttccg gtcatcaagc cttcaataac
    46501 ccattgcgga tcagcatctt ttaatacaat tgccgggttt ttaccgccca gttccagcgt
    46561 tacacgcgtt aagtgatcag cagcagttct ggcaatacct tttcccgtcg cggttgaacc
    46621 ggtaaaactg attttcgcaa catgaggatg tgacgtcagg gccgcgccgc atacagcacc
    46681 tgacccggtg acgacattaa aaacgccatc agggatacca gcctcgctgg ccagttccgc
    46741 cacgcgcaac atcgtcagtg gcgtggtttc cgaaggctta atcacgattg aacagcctgc
    46801 tgccagtgct ggcatcacct tccacatacc aatcatcaac ggaaagttcc atggcacaat
    46861 tcccgccact acgccaaccg gctctttacg cgtccaggcc tgataacgcg ccccctgggg
    46921 taagggaatc gacaagtcca gcgttttacc cgcgattttg gtcgttaacc cggcggtata
    46981 acgcatccag ttcagcgtac agcccacttc aaaagcacgg gaaatggcaa ttgacttgcc
    47041 ttgctccagg gtttccagtt gcgccagctc ctcactgtgc tgttccacca gatcggcaaa
    47101 acgtagcaga atacgttcac gctctgcggg taatcgcccg gcccagcgac gcgagacaaa
    47161 ggcccgccag gcagacatga ctgcgttatc tacatccgct tcgttggcat cagcagtaga
    47221 cgcaatttct tgcccggtgg ccggatcaaa gatcgccaac cgtttttcac tttgtgcggg
    47281 gccaggacga ccatcaatat aaagaccgtg ttgacgatcg agaaactgtt ggacctggct
    47341 taatactgct acatgcggct ctgtcatcac ttttccttat tatttaccca gtgtgatttc
    47401 agtgtacgca gcgacaagaa aagtcacttt tttcgctgtg acattcgctt tgtcagctgt
    47461 gacaagctcc gcaaatcgtg acaataaccg cttatgtctc tatgcctgac aagacttatt
    47521 tcctatagtc aggctcatta gcttcgttta ttgcaacaca aatgcaacaa taaaaataca
    47581 tttcacagag cgaaagcgtg ccatgaaccc cgcaatggat aatgagtttc agcaatggct
    47641 ttcccaaatc aatcaggtat gcggaaattt taccggacgc ctgctgactg agcgttacac
    47701 gggtgtactg gacacgcatt ttgccaaagg actaaagctg agtaccgtga caaccagcgg
    47761 ggtgaattta tcccgcacct ggcaggaggt aaaaggcagc gacgatgcct ggttttacac
    47821 cgtttttcag cttagtggtc aggcaataat ggagcaggat gagcgtcagg tgcagattgg
    47881 cgctggcgat attacgttac tcgatgcctc acgcccctgt tcgctttact ggcaggagtc
    47941 ttctaaacag atttcattac ttttgccacg cactctgctg gaacaatatt ttccccatca
    48001 aaaacctgtc tgcgcagaaa gactggacgc tgacttaccc atggtgcaac tcagtcatcg
    48061 cctgttacag gagagcataa ataatccggc actttctgaa acagaaagtg aagctgcgct
    48121 acaggcgatg gtgtgtctgc tgcgcccggt acttcatcag cgggaatctg ttcaacctcg
    48181 tcgtgaacgt cagtttcaaa aagtggttac gttgatagac gataatattc gcgaagagat
    48241 attacgcccg gagtggatag ccggagagac aggtatgtca gtacgtagtt tgtaccgaat
    48301 gtttgccgat aaaggtttgg tggtcgcgca atatattcgt aaccgtcgtc tcgatttttg
    48361 tgcagatgcg attcgccatg ccgcagatga tgaaaaactg gcaggcatcg gctttcattg
    48421 gggattttct gaccagagtc atttttcaac ggtatttaag caacgctttg ggatgacgcc
    48481 aggcgagtat cgacgtaaat tccgctaact aattggcgtt gtgtgtactg caacgccaat
    48541 tagttaaaac agatatttga ctatgcattt ttcagggcga aaggcaattt gacaggagtt
    48601 tttgaatatt caccttgttt caggaaaata gatcaatcgc tttctcctgt agcgttatcc
    48661 cgatattgcg gttttgctat taaaattttc gcacccattt accgtttatc ccttgtacat
    48721 attcccccgg tttggcccga gctacgagct tttgcccggc gagtttcgcg atatcatcta
    48781 cagacacatt attttgcttt gccagttgtt ggtaactcgc tttacgttcg gcattaatgt
    48841 cggttactaa tttctctgtt tcagcatccg ttttcagcgc aaccagataa ccataaaatg
    48901 tttcacctac ccgcccctga gttctggctt catccagagt caatgccatt acattgctgc
    48961 ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt cttcatcatc gcctcagaaa
    49021 agatcgctac gagtttcaag cagctcttcg acatctttgt ctgccttgat gatgatctca
    49081 tgctcaattt taacgttcat attgatagtg atcggttcct tgggcgcagc gacttcaata
    49141 cgcggcgtac aggcagccag cataaaagat gacgtcaacg cagccagtaa aattttcatt
    49201 gtttttcctc acactctttt ccttgcggac agtcatttcc cggcagacgt gcgttctgct
    49261 ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg ccacagcgta aaaatattct
    49321 cttcatgatg gtaattaagg tttaccgtac cgcttttacc atcaacgcga ctggtacctg
    49381 taatgttggc ctgcatggtt aataaaccga gattatctaa attaatattt gtcgatgaac
    49441 ggctaatttc catatagcgc aaccagttaa ttgctgaacc cgcagtcata ttgtctttca
    49501 ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat tggcccgcta tttgccagcc
    49561 aaccatcttt cactatccat ttttcgttgt ttagccataa cggcaatgca ccactaaatg
    49621 ccccggacat ggcgaattgt ttcggattga cggcgctaac cagttcgctg gatgagagat
    49681 tattcagccg caacagcgcc gggtcatgtt gcggcatacg taattgtttc atcagtacgt
    49741 taccgcctaa cacatcgacg ctaacatcag tcaacagcaa gggttcttct tcagtccacg
    49801 gataacgccc ttgcaaatca gccgtaatat ttttcgctgt caccagatta atcacttcgg
    49861 caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca ggctccatcg gcaaaacgga
    49921 aaggcaggac aaaatcgaca ccgttaacct gattatctgg catccaggca ctaccgcctt
    49981 tcaacacgcc gtgtcctccc gcgcggaatc cttgttcagg cgcagcagaa aatgcaacct
    50041 gagcatacag ttcaccatcg cgtaagttca tcttccagtc gggtggcacc agcggctgga
    50101 atacagtcag tgattgtttt ggccaccagg cgttaccgcg cagacgaata ccgtcccagc
    50161 gaccatttac ccgaaccggg ccaatctcac cagcatgtaa atcgccttta aagaggaaat
    50221 acgtcggatc gcgcccatcg acgctaaatt ttaaggttga tggcggtaac acactgccgc
    50281 cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc gctgaaggag ggatgctgtg
    50341 cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg cggcttttct accgtcatcg
    50401 taccgtattg taactggtcg aaaccggtgg agaggtcggt cagcgtaatc gtgctgtcat
    50461 gccattcacc ttttcctgcg acatcccagg tggcattcat cggtgtaaaa ctgccttttc
    50521 cccagtagcg ccactgccag cggcccgcgt cagggagaaa atcattcgcc agcccatcca
    50581 tatgcagcac gaaatcgccc agttcatttt catgcgcctg caagatggcc tgcaaacgtc
    50641 cgtcaacacc acgttgggtg acttttacac ccgctaaagg ccagcggatt tcatcgatgt
    50701 ccagcgaatc gatgactctt cccttcgaac gtaataacgc gcctggctca aaggccaacg
    50761 ttgggtcagt cagacttccg cttagctgcg caggtaaacg ggcatataaa atgaggtccg
    50821 cctgtttcgc ttcaccggtc agttgcagag gcagctgact gttatccatg cttaattttc
    50881 ctgggccaaa attaagcacc gcgttgcctt tacccgcttg cccctgggtc agcacactca
    50941 gtcgtccgct gaccagagcg ttctcgagcc ctgcctgcca gttgtcgact ttgacaccca
    51001 gtcggccact caaaggaaaa cctgcatacg gccagctcca gcgaccatcg cttacggtca
    51061 attgttgacg agtaatttgc cacggcaaat cgagcaacgg atcgccgtta tcccgtgcca
    51121 gcacgatcaa ttgcccgcta ttttcctgcc agtccagctc ggcatccacc agtgaaggct
    51181 cctgcggcaa gtttaacgtc gcagtagcat gaccacttac aggaagtcca tccggcacga
    51241 gcggcatagt aaattccccc accagtttaa ccggcgactg attttcaaac gcgacgacat
    51301 ccagttcgct gactgtaagt tgttgccctt tcagctggcc ttgaaattta actttttcgc
    51361 cctgataacg cagttgctgg atatcagagg ttaatgcgag agagagtttt ccctgccatt
    51421 cctgccaggg agaaaaaatc agtttatcga tattgatcca ggtgttaggc agcatggact
    51481 gccactgcgc gagggttttt ggagcagctg gcgattgctc cgtctgcggc aatttcgcca
    51541 gacaagcaga atcaagttct accgtgccga cgttcaataa ccagcggctg ggatgtgaaa
    51601 ggctggcgtt ggtgatatgc gcaagctgac aatctcccac cagataacgg agatcgggaa
    51661 tgattaaacc tttacgcgta atgcgtgggc tttcatctaa tgcaatacgt gtcccgagcg
    51721 gtagccagat gcccgccagc gtgggaaccc acagcccgag cgtcatcagc agcgtcaacg
    51781 gcacaagaat aatcagtaat aacagcgcga gaacggcttt atatttaccc agcatgggta
    51841 gttaatatcc tgatttagcg aaaaattaag cattcaatac gggtattgtg gcatgtttaa
    51901 ccgttcagtt gaaggttgcg cctacactaa gcatagttgt tgatgaattt ttcaatatcg
    51961 ccatagcttt caattaaatt tgaaattttg taaaatattt ttagtagctt aaatgtgatt
    52021 caacatcact ggagaaagtc ttatgaaact cgccgtttat agcacaaaac agtacgacaa
    52081 gaagtacctg caacaggtga acgagtcctt tggctttgag ctggaatttt ttgactttct
    52141 gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa gcggtatgta ttttcgtaaa
    52201 cgatgacggc agccgcccgg tgctggaaga gttgaaaaag cacggcgtta aatatatcgc
    52261 cctgcgctgt gccggtttca ataacgtcga ccttgacgcg gcaaaagaac tggggctgaa
    52321 agtagtccgt gttccagcct atgatccaga agccgttgct gaacacgcca tcggtatgat
    52381 gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt acccgtgacg ctaacttctc
    52441 tctggaaggt ctgaccggct ttactatgta tggcaaaacg gcaggcgtta tcggtaccgg
    52501 taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt ggtatgcgtc tgctggcgtt
    52561 cgatccgtat ccaagtgcag cggcgctgga actcggtgtg gagtatgttg atctgccaac
    52621 cctgttctct gaatcagacg ttatctctct gcactgcccg ctgacaccgg aaaactacca
    52681 tcttttgaac gaagccgcct tcgatcagat gaaaaatggc gtgatgatcg tcaataccag
    52741 tcgcggtgca ttgattgatt ctcaggcggc aattgaagcg ttgaaaaatc agaaaattgg
    52801 ttcgttgggt atggacgtgt atgagaacga acgcgatctg ttctttgaag ataaatccaa
    52861 cgacgtgatc caggatgacg tattccgtcg cctgtctgcc tgccacaacg tgctgtttac
    52921 cgggcaccag gcattcctga cagcagaagc tctgaccagt atttctcaga ctacgctgca
    52981 aaacttaagc aatctggaaa aaggcgaaac ctgcccgaac gaactggttt aatcttgccg
    53041 ctcccctgca ttccagggga gcttattcag ataatcccca aagacctttc atcttctatt
    53101 cttaaaatag ccctgagtca gaaactgtaa ttgagaacca caatgaagaa agtagccgcg
    53161 tttgttgcgc taagcctact gatggcggga tgtgtaagta atgacaaaat tgctgttacg
    53221 ccagaacagc tacagcatca tcgctttgtg ctggaaagcg taaacggtaa gcccgtgacc
    53281 agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa tgatgatttc cggcagcatg
    53341 tgtaaccgct ttagcggtga aggcaaactg tctaatggtg aactgacagc caaagggctg
    53401 gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg aactcgataa caccattagc
    53461 gaaatgctga aagaaggtgc acaagtggat ctgaccgcga accagttaac gctggcgacc
    53521 gcgaaacaga cattaactta taagctggcg gatttaatga attaatagct gccacagctc
    53581 ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg gtaatgcaca catcccaatc
    53641 gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc cgatcattgc acaatttgct
    53701 tctccactac tggacatcga cgcttttaaa cctggcgcta cgtgcgccgc agtcgcctgc
    53761 tgaacaggtt cactactaca cgccgacaac aataaagcgg cacaccctac ccaaaacgct
    53821 gctcgcatct cttcttcctc tgatcttcaa gccaaacgac accgccataa ataataggca
    53881 gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc cgcattttta cttttttatg
    53941 gctatttttt tgccctctgt ttgatcaaaa cattcattac gctgatgtgg gggacacaaa
    54001 agcgaaaatg cagaagaaag ccatttgcta aaattgaagg attactactg ggcgcgcagc
    54061 aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca tatgattact attgacggta
    54121 atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt tatcgccatc taccctatta
    54181 cccccagttc cacgatggca gaacaggctg atgcctgggc cggaaacggc ttgaagaacg
    54241 tttggggaga cacaccacgc gtggttgaaa tgcagtcgga agcgggtgct atcgctaccg
    54301 tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt tacgtcatcg cagggtttgc
    54361 tgctgatgat cccgacgctg tacaaactgg caggcgaact cacaccgttt gtcctgcatg
    54421 tagcggcacg taccgttgcc acacatgcac tctctatttt tggcgatcat tccgacgtca
    54481 tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc aaacgtccag gaagcgcaag
    54541 actttgctct catttcgcac atcgcgacgc tgaaaagccg cgtgccattt attcatttct
    54601 ttgatggttt ccgcacgtcc cacgaaatca acaaaattgt cccgctggcc gatgacacga
    54661 ttcttgatct catgccgcag gccgaaattg atgctcatcg cgcccgggca ctcaacccgg
    54721 aacatccggt gatccgcggt acgtccgcca atcctgacac ttatttccag tctcgcgaag
    54781 ccaccaaccc atggtacgac gcggtctatg accatgttga acaggcgatg aatgatttct
    54841 ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta cgggcatccg caagcggaac
    54901 gggtgattat cctgatgggc tctgccattg gcacctgtga agaagtggtt gatgaattgc
    54961 taatccgtgg cgaaaaagtc ggcgtgctga aagttcgcct gtaccgcccc ttctccgcca
    55021 aacacttgct gcaagctctg ccgggatccg tacgcagcgt ggcggtactg gacagaacca
    55081 aagaacccgg tgcccaggca gaaccgctct atctggatgt gatgaccgca ctggcagaag
    55141 cctttaataa tggtgagcgc gaaaccctgc cccgtgtcat tggtgggcgc tatggtcttt
    55201 catccaaaga atttggcccg gactgtgtac tggcggtatt tgccgagctc aacgcggcta
    55261 aaccgaaagc gcgctttacg gttggtattt acgatgatgt gactaatctg tcactgccgt
    55321 tgccggaaaa caccctgcca aactcggcga aactggaagc cttgttttat ggccttggta
    55381 gtgatggcag cgtttccgcg accaaaaaca atatcaagat tatcggtaat tccacgccgt
    55441 ggtacgcaca gggctatttt gtttacgact ccaaaaaagc gggcggcctg acggtttctc
    55501 accttcgcgt gagcgaacag ccgattcgtt ccgcttatct catttcccag gctgattttg
    55561 ttggctgcca ccagttgcag tttatcgata aatatcagat ggctgagcgt ttaaaacctg
    55621 gcggcatttt cctgctcaac acaccgtaca gcgcagatga agtgtggtcg cgcttgccgc
    55681 aagaagttca ggccgtgtta aaccagaaaa aagcgcgctt ctatgtgatt aacgcggcga
    55741 aaatcgcccg cgaatgtggc ctggcggccc gtattaatac cgtcatgcag atggcttttt
    55801 tccatctgac gcaaattctg cctggcgata gcgccctcgc agaattgcag ggtgcgattg
    55861 ccaaaagtta cagtagcaaa ggccaggatc tggtggaacg caattggcag gctctggcgc
    55921 tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt aaatccgcac agcgccaatc
    55981 gaccgccagt ggtttccgat gccgctcctg atttcgtgaa aaccgtaacc gctgcgatgc
    56041 tcgccgggct tggtgacgcc ctccccgttt cggcgctgcc gccagacggc tcctggccga
    56101 tgggcactac gcgctgggaa aaacgtaata ttgccgaaga gatccccatc tggaaagagg
    56161 aactctgtac ccaatgtaac cactgcgttg ccgcttgccc acactcagct attcgcgcaa
    56221 aagtggtgcc gcctgaagcg atggaaaacg cccctgccag cctgcattcg ctggatgtga
    56281 aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt ggctccggaa gattgcaccg
    56341 gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg tcagaatcca gagattaaag
    56401 ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga gaaaatcaat tacgatttct
    56461 tcctcaacct gccagaaatc gaccgtagca aactggaacg tattgatatt cgtacatcgc
    56521 agctgattac accgctgttt gaatattcag gtgcttgctc cggttgtggc gagacgccgt
    56581 atattaaatt actgactcag ctctatggcg accggatgtt gatcgctaac gccaccggct
    56641 gttcttcaat ttatggcggt aacctgccct ctacaccgta taccaccgat gccaacggtc
    56701 gtgggccggc atgggcgaac tctctatttg aagataatgc cgaatttggc cttggtttcc
    56761 gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct gctggatcaa tttgccgata
    56821 aaatcccggc ggaattactg acggcgttga aatcagacgc tacgccagag gttcgtcgag
    56881 aacaggtcgc agctttacgc cagcaactca acgatgttgc cgaagcacat gaactgctac
    56941 gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat tggtggtgat ggctgggctt
    57001 acgatatcgg ctttggcggt ctggatcatg tattgagttt gacggaaaac gtcaacattc
    57061 tggtgctgga tacgcaatgc tattccaaca ccggtggtca ggcatcgaaa gcgacaccgc
    57121 tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa agcgcgtaaa gatcttggcg
    57181 tcagtatgat gatgtacggt catgtttatg tggcgcagat ttctctcggc gcgcagctga
    57241 accagacggt gaaagcgatt caggaagcgg aagcgtatcc ggggccatcg ctgatcattg
    57301 cttatagccc gtgtgaagag catggttacg atctggcact cagccacgac cagatgcgcc
    57361 aactcacagc taccggcttc tggccgctat atcgcttcga tccgcgtcgt gccgatgaag
    57421 gcaaactgcc gctggcgctg gattcacgcc cgccgtcagt agcactggaa gaaacgttac
    57481 ttcacgagca acgtttccgt cggctgaatt cgcagcagcc agaagtggca gaacagttat
    57541 ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt cctggcacaa atggccggaa
    57601 aagcggaaaa aagcaacacc gattaaatgc tctggataag gattatccaa ttctaaaaaa
    57661 aaaagcccgg acgactgttc gggcttgtct ttttatatgt tg
//
LOCUS       DAFNBJ010000009        57331 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000009 DAFNBJ010000000
VERSION     DAFNBJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.009"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..138
                     /locus_tag="L2X49_000568"
     CDS             <1..138
                     /locus_tag="L2X49_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HBN4795709.1"
                     /translation="DMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEV
                     K"
     gene            complement(179..649)
                     /locus_tag="L2X49_000569"
     CDS             complement(179..649)
                     /locus_tag="L2X49_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBN4795710.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(696..1415)
                     /gene="yehT"
                     /locus_tag="L2X49_000570"
     CDS             complement(696..1415)
                     /gene="yehT"
                     /locus_tag="L2X49_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBN4795711.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1412..3097)
                     /gene="btsS"
                     /locus_tag="L2X49_000571"
     CDS             complement(1412..3097)
                     /gene="btsS"
                     /locus_tag="L2X49_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBN4795712.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3319..4050
                     /gene="mlrA"
                     /locus_tag="L2X49_000572"
     CDS             3319..4050
                     /gene="mlrA"
                     /locus_tag="L2X49_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HBN4795713.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            4110..4217
                     /locus_tag="L2X49_000573"
     CDS             4110..4217
                     /locus_tag="L2X49_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBN4795714.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(4198..4929)
                     /gene="yehW"
                     /locus_tag="L2X49_000574"
     CDS             complement(4198..4929)
                     /gene="yehW"
                     /locus_tag="L2X49_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBN4795715.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(4934..5860)
                     /gene="yehX"
                     /locus_tag="L2X49_000575"
     CDS             complement(4934..5860)
                     /gene="yehX"
                     /locus_tag="L2X49_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBN4795716.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(5853..7010)
                     /gene="yehY"
                     /locus_tag="L2X49_000576"
     CDS             complement(5853..7010)
                     /gene="yehY"
                     /locus_tag="L2X49_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBN4795717.1"
                     /translation="MTYLRINPVLALLLLLTVIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSIFALILVQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(7017..7934)
                     /gene="osmF"
                     /locus_tag="L2X49_000577"
     CDS             complement(7017..7934)
                     /gene="osmF"
                     /locus_tag="L2X49_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBN4795718.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8145..10442)
                     /gene="bglX"
                     /locus_tag="L2X49_000578"
     CDS             complement(8145..10442)
                     /gene="bglX"
                     /locus_tag="L2X49_000578"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBN4795719.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGVLYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            10638..12353
                     /gene="dld"
                     /locus_tag="L2X49_000579"
     CDS             10638..12353
                     /gene="dld"
                     /locus_tag="L2X49_000579"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBN4795720.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12391..13323)
                     /gene="pbpG"
                     /locus_tag="L2X49_000580"
     CDS             complement(12391..13323)
                     /gene="pbpG"
                     /locus_tag="L2X49_000580"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBN4795721.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13497..14084)
                     /locus_tag="L2X49_000581"
     CDS             complement(13497..14084)
                     /locus_tag="L2X49_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBN4795722.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14254..14832
                     /locus_tag="L2X49_000582"
     CDS             14254..14832
                     /locus_tag="L2X49_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBN4795723.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(14962..15723)
                     /locus_tag="L2X49_000583"
     CDS             complement(14962..15723)
                     /locus_tag="L2X49_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBN4795724.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(15776..17212)
                     /gene="mdtQ"
                     /locus_tag="L2X49_000584"
     CDS             complement(15776..17212)
                     /gene="mdtQ"
                     /locus_tag="L2X49_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBN4795725.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQACFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17436..17519
                     /gene="yohP"
                     /locus_tag="L2X49_000585"
     CDS             17436..17519
                     /gene="yohP"
                     /locus_tag="L2X49_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBN4795726.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(17890..18837)
                     /gene="dusC"
                     /locus_tag="L2X49_000586"
     CDS             complement(17890..18837)
                     /gene="dusC"
                     /locus_tag="L2X49_000586"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBN4795727.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            19076..19474
                     /locus_tag="L2X49_000587"
     CDS             19076..19474
                     /locus_tag="L2X49_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBN4795728.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19471..20166
                     /locus_tag="L2X49_000588"
     CDS             19471..20166
                     /locus_tag="L2X49_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBN4795729.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            20296..21180
                     /gene="cdd"
                     /locus_tag="L2X49_000589"
     CDS             20296..21180
                     /gene="cdd"
                     /locus_tag="L2X49_000589"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBN4795730.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVTLECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            21330..22049
                     /gene="sanA"
                     /locus_tag="L2X49_000590"
     CDS             21330..22049
                     /gene="sanA"
                     /locus_tag="L2X49_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBN4795731.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            22052..22291
                     /locus_tag="L2X49_000591"
     CDS             22052..22291
                     /locus_tag="L2X49_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBN4795732.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            22610..23848
                     /gene="preT"
                     /locus_tag="L2X49_000592"
     CDS             22610..23848
                     /gene="preT"
                     /locus_tag="L2X49_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HBN4795733.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKMLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSNVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            23842..25077
                     /gene="preA"
                     /locus_tag="L2X49_000593"
     CDS             23842..25077
                     /gene="preA"
                     /locus_tag="L2X49_000593"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBN4795734.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASSMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(25148..26158)
                     /gene="mglC"
                     /locus_tag="L2X49_000594"
     CDS             complement(25148..26158)
                     /gene="mglC"
                     /locus_tag="L2X49_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBN4795735.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(26174..27694)
                     /gene="mglA"
                     /locus_tag="L2X49_000595"
     CDS             complement(26174..27694)
                     /gene="mglA"
                     /locus_tag="L2X49_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBN4795736.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPCARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(27755..28753)
                     /gene="mglB"
                     /locus_tag="L2X49_000596"
     CDS             complement(27755..28753)
                     /gene="mglB"
                     /locus_tag="L2X49_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBN4795737.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(29033..30073)
                     /gene="galS"
                     /locus_tag="L2X49_000597"
     CDS             complement(29033..30073)
                     /gene="galS"
                     /locus_tag="L2X49_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBN4795738.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(30215..31372)
                     /locus_tag="L2X49_000598"
     CDS             complement(30215..31372)
                     /locus_tag="L2X49_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBN4795739.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLVGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(31389..32057)
                     /gene="folE"
                     /locus_tag="L2X49_000599"
     CDS             complement(31389..32057)
                     /gene="folE"
                     /locus_tag="L2X49_000599"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBN4795740.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            32315..33151
                     /gene="yeiG"
                     /locus_tag="L2X49_000600"
     CDS             32315..33151
                     /gene="yeiG"
                     /locus_tag="L2X49_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBN4795741.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(33183..35174)
                     /gene="cirA"
                     /locus_tag="L2X49_000601"
     CDS             complement(33183..35174)
                     /gene="cirA"
                     /locus_tag="L2X49_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBN4795742.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSFNYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(35468..36937)
                     /gene="lysP"
                     /locus_tag="L2X49_000602"
     CDS             complement(35468..36937)
                     /gene="lysP"
                     /locus_tag="L2X49_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBN4795743.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(37142..38023)
                     /locus_tag="L2X49_000603"
     CDS             complement(37142..38023)
                     /locus_tag="L2X49_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4795744.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            38122..39171
                     /locus_tag="L2X49_000604"
     CDS             38122..39171
                     /locus_tag="L2X49_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBN4795745.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            39245..40102
                     /gene="nfo"
                     /locus_tag="L2X49_000605"
     CDS             39245..40102
                     /gene="nfo"
                     /locus_tag="L2X49_000605"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBN4795746.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            40105..41193
                     /locus_tag="L2X49_000606"
     CDS             40105..41193
                     /locus_tag="L2X49_000606"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBN4795747.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(41249..42499)
                     /locus_tag="L2X49_000607"
     CDS             complement(41249..42499)
                     /locus_tag="L2X49_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBN4795748.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(42599..43540)
                     /gene="rihB"
                     /locus_tag="L2X49_000608"
     CDS             complement(42599..43540)
                     /gene="rihB"
                     /locus_tag="L2X49_000608"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBN4795749.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            43670..44368
                     /gene="yeiL"
                     /locus_tag="L2X49_000609"
     CDS             43670..44368
                     /gene="yeiL"
                     /locus_tag="L2X49_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBN4795750.1"
                     /translation="MKEIHNNDLKLQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(44565..45815)
                     /locus_tag="L2X49_000610"
     CDS             complement(44565..45815)
                     /locus_tag="L2X49_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBN4795751.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(45909..46847)
                     /gene="psuG"
                     /locus_tag="L2X49_000611"
     CDS             complement(45909..46847)
                     /gene="psuG"
                     /locus_tag="L2X49_000611"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBN4795752.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(46835..47776)
                     /locus_tag="L2X49_000612"
     CDS             complement(46835..47776)
                     /locus_tag="L2X49_000612"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBN4795753.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(48200..49891)
                     /gene="fruA"
                     /locus_tag="L2X49_000613"
     CDS             complement(48200..49891)
                     /gene="fruA"
                     /locus_tag="L2X49_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBN4795754.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(49908..50846)
                     /gene="fruK"
                     /locus_tag="L2X49_000614"
     CDS             complement(49908..50846)
                     /gene="fruK"
                     /locus_tag="L2X49_000614"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBN4795755.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(50846..51976)
                     /gene="fruB"
                     /locus_tag="L2X49_000615"
     CDS             complement(50846..51976)
                     /gene="fruB"
                     /locus_tag="L2X49_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBN4795756.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGGDAEQALKAIGDAIAAGLGEGA"
     gene            52344..53525
                     /gene="setB"
                     /locus_tag="L2X49_000616"
     CDS             52344..53525
                     /gene="setB"
                     /locus_tag="L2X49_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBN4795757.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFSMVMIIATLFCLLRIKD
                     V"
     gene            complement(53522..53776)
                     /locus_tag="L2X49_000617"
     CDS             complement(53522..53776)
                     /locus_tag="L2X49_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBN4795758.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            53931..54503
                     /gene="yeiP"
                     /locus_tag="L2X49_000618"
     CDS             53931..54503
                     /gene="yeiP"
                     /locus_tag="L2X49_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBN4795759.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFIDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            54726..56192
                     /locus_tag="L2X49_000619"
     CDS             54726..56192
                     /locus_tag="L2X49_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBN4795760.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            56310..57296
                     /gene="yeiR"
                     /locus_tag="L2X49_000620"
     CDS             56310..57296
                     /gene="yeiR"
                     /locus_tag="L2X49_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBN4795761.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
BASE COUNT        14031 a        15303 c        14185 g        13812 t
ORIGIN      
        1 gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa cgtttctgct
       61 gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa agccgctggt
      121 tttaaagaag tgaaataatc tgtcggcggc catgtttcgc atggccgcca tacccgtctt
      181 agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt aaaaattgat
      241 cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta atttccggca
      301 tcgaaacgcg gaactgctgt tcggtgagga tagccagaat gtcatcggtt ttcagcgaaa
      361 acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc ggctccagtg
      421 ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg aatgacgaca
      481 aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc cagacggcga
      541 tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg tcattattat
      601 tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg aggccttcag
      661 gtgttgatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc ctcttttaag
      721 cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat caactcggcc
      781 tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag atactgacga
      841 tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc ggtaaagccc
      901 tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac aaatgccaca
      961 tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa tttcagcgcc
     1021 tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gttcctgacg caaacgcgcc
     1081 agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa ggcatgttct
     1141 tcaaaggctt taatagcgta ttcgtcaaac gcggtgagaa aaacaatata cggacgatgt
     1201 tccgggtcga gcatccccac catttccaga ccactgatgc gcggcatctg gatatcgaga
     1261 aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc gtttgaacac
     1321 tctccaacga tttcaatatc gctctgctcc tgcaaaaata cgcgcaggtt ctcccgtgct
     1381 aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat ggtagtcgta
     1441 acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag tcatcgccaa
     1501 accgttcacg taaacgctta tccaccagat tcatccccag cccactggca ttggttaccg
     1561 gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc tcacgtcggg
     1621 cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta atggcgtttt
     1681 ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct tgcggaatag
     1741 caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga taagcgttca
     1801 catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag tttttgcgga
     1861 aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg cggatcaccg
     1921 ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg tgaagcagtt
     1981 tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca agaatctgcg
     2041 ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg aataaacggt
     2101 ttttggcttc atacaatttg atggtgccca tcacgcgctg attttcgcca cgcaacggaa
     2161 ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa ggtacttcgt
     2221 tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa gtcgaagaaa
     2281 tcggtttgcc gggtaaatgg tggtcgtcac caattccggt aaaggccagc aatttctctc
     2341 gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctga gccactttca
     2401 tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct gccactttca
     2461 gcgcagtggc agaaaaagca gaagtgtatt tttcaaacat cgcgcgttta tcgagcaata
     2521 tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggtgca gcaatattac
     2581 tcaccagacg caccgcatct tcataaggtc gggcgatcgc aaggatgatc agcatttgca
     2641 ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact ttatcagtgc
     2701 gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag caatccttca acgatggttg
     2761 agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag ccgccggtca
     2821 gaccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc gcgcctatcg
     2881 cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc atgatgcaga
     2941 agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg acctgcatta
     3001 acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa acgcacatct
     3061 gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac acttcccttt
     3121 aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca gaaaaatgag
     3181 aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc tatagatgca
     3241 caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa ccgggacctc
     3301 gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt gctttgtgat
     3361 attaaccctg tcacgttacg cgcgtggcag aggcgttacg ggttgctgaa accgcaacgg
     3421 acagacggcg gtcatcgact gttcaacgat gccgatattg accgtatccg cgagatcaaa
     3481 cgctggatcg acaacggcgt gcaggtcagc aaagttaaaa tgctgctcag taatgaaaat
     3541 gttgatgtgc agaacggctg gcgcgatcag caagaaacat tactgaccta cctgcaaagc
     3601 ggcaatctgc atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
     3661 acactcacca cacatctgtt tattcctctg cgccgacgac ttcaatgcca acaaccgact
     3721 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
     3781 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
     3841 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
     3901 ctcgcccact cgctcaatca actacgccct gaactattcg aaggccgtac attgctggtg
     3961 tggtgcggtg aaaatcgaac ctccgcccaa cagcagcaac tcaccagttg gcaagaacaa
     4021 ggccatgata ttttcccact cggcatttaa tgattcgtta acaaatgcgc tttactgtac
     4081 aatcctttcg ttaacataag gagtgcaata tgcgcatagc taaaattggg gtcatcgccc
     4141 tgttcctgtt tatggcgtta ggcggaattg gtggcgtcat gctcgcaggt tataccttta
     4201 ttttgcgtgc tggctaagcg cctgcaccag cctttcaaac aggcggtctg cgatgatcgc
     4261 cgccagtgcc accagtaacg ccccctggat cacatacgcg gtattaaatc cgctaagccc
     4321 gatgatgatg ggcgtaccca gcgtgctggc ccctaccgtt gaggcgatcg tcgccgtacc
     4381 aatgttgata atcaccgaag ttcgcacgcc cgccagaatc accggagccg ccagcggtag
     4441 ctcgacctta cgcagtcgct gaccacgact cattcccata cctttcgcaa cttctgtcac
     4501 gctggcatca atcgctccca gcccggcaag tgtcgcctgc aggacgggca gcacaccgta
     4561 aaggatcaag gcgataatcg ctggttgcag accaaagccg atcaccggaa cggcaatcgc
     4621 cagcactgcg acaggcggaa aagtctgtcc aacggcggca atagtttcca ccagtgggcg
     4681 aaattccgcg ccccacgggc gagtgacagc aattccggca ccagtgccaa tgatcaccgc
     4741 aaacaaactc gaaattccca ccagccagaa atgagccagt gccagagctg caaaactttc
     4801 ttgctgataa acgggtcgtg gcagttgtgg gaacaaggca gcaaacagcg gctggctgta
     4861 aggcagccag aaaatcagtg ccacaaacaa agcaatgagc cagaacagcg gatcgcgcaa
     4921 catcttcata cgcttacgcc tcctccagca gatcctgaaa atgcagcgtg ccgcaaggct
     4981 ggccctgcgt gttcaccacc ggcagcacct cgcatccccg cgcgacaaac agggagagcg
     5041 catcgcgtag cgtcatctct tctgccagtg cctcaccttc tgcccgttct tcgcgacgca
     5101 cgtaatccgc cacactacgt aacgaaagca ggcgcacacc cagttcacta cgtccaaaaa
     5161 actggcggac aaaatcattc gccggacgag tcagcatcgt cagcggattc ccctgctgca
     5221 ccacttcacc gtgatccatc aataccagat gttctgccag ccttagcgcc tcatcaatat
     5281 catgagtgac cagcacaata gtgcgcccca gcaaacggtg aatgcgcgtc atctcttgtt
     5341 gcaacgcgcc gcgcgttacc gggtccagtg cgccaaaagg ttcatccatc agtaagactt
     5401 gcggatcggc agccagtgcg cgcgccactc ccacacgttg ctgctgacca ccggaaagct
     5461 gatgcggata acgctcacgc aaatttgact ccagccccag tagcgccatt aattcgtcga
     5521 tacgatcgtc aatccgcgcc cgcgaccatt tttgcaattg cggcacggta gcgatgtttt
     5581 gcgccacgct ccagtggggg aacaggccaa tagattgaat ggcatagccc atccggcggc
     5641 gcaactccag tactggcagc gagcgaattt cttctccggc aaagcggatc acgccgctgt
     5701 catgttccac taggcggtta atcattttca gggtggtgga tttgccggag ccagatgtgc
     5761 caatcagcac cgaaaaactc ccttcctgaa aattgagatt gagatcgtta acggcttttt
     5821 gtgcgccaaa cagtttgctg acatggctaa attcaatcat tacgtttcac cttcagcagt
     5881 gcgataagca aatcgaacag cgcgtcggta agcaccgcca gaacaattac cgggatcacc
     5941 cccagcaaca ctaaatcaat ggcgctgctt agcagcccct ggaaaaccag cgcaccaaaa
     6001 ccgcctgcgc cgattaacgc cgcaatcacc gccataccta cagtttgcac catcaccacc
     6061 cgcaggctgc gcagaaatac cggtaacgcc agcggtaact gaacatgcag gaaacgctgc
     6121 gccccgctca tccccatcgc tctggcgctc tccagcacat cgcgcgggat ctggttcaaa
     6181 ccgactacca cgccgcgcac cagcggcagc aaggcataga gcaccagcgc aatcagtgcg
     6241 ggtgtcattc cggttcctgc tatgccgagc ttccccagcc acggaaaggc cgtaaccagc
     6301 gcggcaagcg gcgcaatcaa caggccaaag agcgccaccg aaggcacggt ctgaatgaca
     6361 ttgagcagag aaaaaattgc cccctgccgc gcagtggaaa agtagcacca gatgcccaac
     6421 ggcacaccaa tcactaacgc aggcagcacc gcaccaaaca gcaacgtcag atgttgtgcc
     6481 agcgcgtcgt caaacacatc ctgacggttg gcgtattctt tcattagtga gagatcgtta
     6541 agcgtgccgg agtacagcaa ccacagcgga ataatggcaa tctgcatatg caacaaccag
     6601 cgccacagcg gatgcgtgga gattcggcgg atggcatcgc tacaggccag caatgccagc
     6661 gccgcagcca gccagaaacc actgccgagg ctggtacgcg ccagcgcact gccattttgc
     6721 gccagttggg tcgccgcctt tccagctccc cacaccagca atacgaagac gaattgcacc
     6781 agaatgagtg caaaaatgct gcctttttta ccgggaataa aacaggctgt cagccaggcg
     6841 caaccaacgc ccaccagcat ccagatcgtt tgcggccaca gctgccagag atgtcgccct
     6901 tcacccgaga ctaaacgatt aggcgcgtaa ctgataaacg gcagcgccgc tgcgattacc
     6961 gtcagcaaca gcagcagcgc cagaacagga ttaatacgta gataagtcac gggaaattac
     7021 ttcgcccacc ctttttgttt caggtagtcg gcagccactt ttttggcatc cagtccttcc
     7081 actgcaatgc tggcattcag ttgctgcaat gtttttgcat cgaggctggc gaagactggc
     7141 tgtagccact gtgccatttg cggatactcc ttcaacaccg attcacgcac cactggtgca
     7201 ggcgcataga taggttgcac gccttgcgga tcgcttaagg tttgcagccc cagcgccgct
     7261 accgggccgt cagtgccgta agccattgca gcattaacgc cagaggtttg ctgggcagcg
     7321 gctttgatcg tcaccgccgt gtcgccacca gccagtgaca gcaactgatc ctgaccgagc
     7381 ttaaagccat aggctttttc aaacgcgggt aacgcatcgg cgcgttcgat aaactctgcc
     7441 gaggctgcca gtttgaaggt gccgccctct tgcagataac gactcaggtc ggcaagcgaa
     7501 gtgagtttat ttttctctgc cacatcctga cgcacggcga tagtccaggt gttatttgca
     7561 ggcgcgggcg tcagccagat taacttgttg tgctccgaat cgagcttttt gactttctcg
     7621 taaccttgct gcgcgttttt ccacgctgca tcgttttcat ctttaaagaa gaaagcgcca
     7681 ttgccggtat attccggata gatatccagt tcaccggaag taatcgcccc gcgcaccaca
     7741 ggagtcgttc cgagttgcac tttattcacc gtcggtacgc cgtggctttc cagcacctgc
     7801 aaaatgatat tgccgagtag cgcaccttcg gtatcgattt ttgaaccgac tttaacgggg
     7861 gaagccgcct gtagcggcag gctcacggct gccaacataa ccagtgaacc tgcccagagc
     7921 tttaagagtg gcatgatgct ttcctcattc ttttactgtt gttttcagcg aattaagaga
     7981 aaagcatagt tgataatggc ggggttagct tgagcggaag gattcagttg cagaatcaga
     8041 taaatactta aggggcatat tcggtccggc gtttcctgcc ggatgcggcg cgagcgcctt
     8101 gtccggccta caaagggcgc aaacgtcgcg cccttactaa agcattacag caactcaaac
     8161 tcgcctttct taacgcgtgc ggaatcagtg ccgataaata cattgaactt gccaggctcg
     8221 gcgtcatatt tcatctgttg attccagaac ttcagcgcct caatatcgat cgggaagctg
     8281 acagtctgag tttcgcccgg tttcagggtg attttctcaa agcctttcag ctgtttcaca
     8341 gggcgactca tggaagccgt cacatcctgc aagtacatct gcactaccgt ggccccctcg
     8401 cgcttaccgg tgttcgtcac ctgcacgctg gcagtcactt tgccgtcacg cttcatggtc
     8461 ggcgcagaaa gtttcacatc agagacggtg aaagtggtgt agctcagccc atagccgaac
     8521 ggatacaaca ccccgttagc ttcatcaaaa taacgcgaag tgtatttgtt cggcttgtcg
     8581 gcattatacg ggcgaccggt attcagatgg ctgtagtaca ccgggatctg cccgacagaa
     8641 cgcgggaagg acattggcag cttgccggac gggttgtaat cgccaaacaa tacatcggca
     8701 attgcattac cgccttcagt ccccgcaaac caggtttcca gaatcgcatc agcctgctga
     8761 tcttctttca ccagcgccag cggacgcccg ttcatcagca ccagcaccag cggtttaccg
     8821 gtggctttca gcgccgcaat caagtcacgt tggctttgcg gaatagtgat atcggtccgg
     8881 ctggaggctt cgtgcgccat cccctgtgct tcaccgacta cagccaccac cacatcagat
     8941 tgtttcgccg tctgcaccgc ttcatcaatc atctcttgcg gcgaacgcgg atcgactttg
     9001 accgcttctt catactgatt caggaaatcg ataatgcctt tgtcactggt aacgttcgcc
     9061 cctttggcat acagcacttt accgttttca ccgacggaat ttttaatccc ggtcagtacg
     9121 gtcacggatt gatcggcaac accggctgcg gaccagctgc ccatcacgtc acgtttactg
     9181 tccgccagtg gcccaaccac cgcaatggtg gccgattttt tcagcggtaa cgtttcgaga
     9241 cggtttttca gcaacaccag gctttcgcgc gccacttcac gcgcttcttt acggtgcagg
     9301 cggctttcgg cattggtatc caccgggtca gactctttcg gccccaaatg gctgtatggg
     9361 tcgttaaaca atcccatatc atatttaacg ttcagtacat ggcgggcagc atcgtccagc
     9421 tcttccatcg tcactttgcc ggatttgatc aacccaggca gatacttcga gtaatactcg
     9481 tcgctcatac tcatgttgat tccggatttc agcgccacgc gcaccgcatc ttccgggtct
     9541 gccgccgtgc catgtttaat cagctctttg attgcaccgt gatcggaaac ggtgatgcct
     9601 ttaaagcccc actggtcgcg cagaacatct ttcagcagcc aggaatcgga ggtggctggc
     9661 gtgccgttca gcgagttcag cgccaccatc accgcgccgc tgcctgcgtc cagccccgct
     9721 ttgtacggcg gcatataatc attaaacagg cgctgcggac tcatatcgac ggtgttgtac
     9781 tctttaccgc cttctaccgc gccgtatgcg gcaaagtgtt tgacgctggt catcaccgag
     9841 tagcgatctg ccgggctttt accctgcatc gcttccacca tggttttacc cattgttgag
     9901 gtgagatacg tatcttcgcc aaaaccttcg gaagcacgtc cccagcgcgg atcgcgcgag
     9961 acatcgacca tcggtgccca ggtcatattc aggccatcat ctgccgcttc ataagcagag
    10021 acacgtccga ccgttttcac tgcatcgagg ttaaaagacg aggccagacc gaggctaatc
    10081 gggaacaccg tgcgctgacc gtgcagcacg tcgtaagcaa agaaaagagg aattttcagg
    10141 cggcttaatt ccatcacctg atcctgcatg gcgcggatat cctgacgggt tacggtgttg
    10201 aaaatcgccc caacctgacc gtctttgatc atctcgcgga tcgcctcttt cgggttatcc
    10261 gggccgacgc tgattaagcg cagctgacca attttctcat caactgtcat tttcttaagc
    10321 agttcggtga cgaacgcatc ccgcgcttcg ggcgttaatg gatggttgcc gaataaatca
    10381 tccgccagtg ctggctgcag ggccagactc accgcgattc ctactgaaca tagccatttc
    10441 atatggattt ttcttcctct gttgcccgac gaggcagcga aaatggtgca aaaaccgtag
    10501 tttgccataa gcatgatgga gagagaaaaa gaatgctcag tttattgtct gaattttcaa
    10561 aatattcact cgctgaattg tcatacaagg cgctattcta gtttgtgata ttttttcgcc
    10621 accacaagga gtggaaaatg tcttccatga caacaactga taataaagcc tttttgaatg
    10681 aacttgcccg tctggtcggt cattcacacc tgctcaccga tcccgcaaaa acggcccgct
    10741 atcgcaaggg cttccgttct ggtcagggcg acgcgctggc tgtcgttttc cctggctcac
    10801 tactggaatt gtggcgggtg ctgaaagcct gtgtcaccgc cgacaaaatt attctgatgc
    10861 aggctgccaa tacaggcctg accgaaggat cgacgccaaa cggtaacgac tatgatcgcg
    10921 atatcgtgat catcagcacc ctgcgtctcg acaagctgca cgttcttggc aagggcgaac
    10981 aggtgctggc ctatccgggc accacgctct attcgctgga aaaagccctc aaaccgctgg
    11041 gacgcgaacc gcactcggtg attggatcgt cgtgtatagg cgcatcggtt atcggcggta
    11101 tttgtaacaa ctccggcggc tcgctggtgc aacgtggccc ggcgtatacc gaaatgtcgt
    11161 tattcgcgcg tataaatgaa gacggcaaac tgacgctggt gaaccatctg gggattgatc
    11221 tgggcgaaac gccggagcag atccttagca agctggatga tgatcgcatc aaagatgacg
    11281 atgtgcgtca cgatggtcgt cacgcccacg attatgacta tgtccaccgc gttcgtgata
    11341 ttgaagccga cacgcccgca cgttataacg ccgatccgga tcggttattt gaatcttctg
    11401 gttgcgccgg gaagctggcc gtctttgcgg tacgtctaga taccttcgaa gcggaaaaaa
    11461 atcagcaggt gttttatatc ggcaccaacc agccggaagt gctgaccgaa atccgccgtc
    11521 atattctggc taacttcgaa aatctgccgg ttgccgggga atatatgcac cgggatatct
    11581 acgatattgc ggaaaaatac ggcaaagaca ccttcctgat gattgataag ttaggcaccg
    11641 acaagatgcc gttcttcttt aatctcaagg gacgcaccga tgcgatgctg gagaaagtga
    11701 aattcttccg tccgcatttt actgaccgtg cgatgcaaaa attcggtcac ctgttcccca
    11761 gccatttacc gccgcgcatg aaaaactggc gcgataaata cgagcatcat ctgctgttaa
    11821 aaatggcggg cgatggcgtg ggcgaagcca aatcgtggtt ggtggattat ttcaaacagg
    11881 ccgaaggcga tttctttgtc tgtacgccgg aggaaggcag caaagcgttt ttacaccgtt
    11941 tcgccgctgc gggcgcagca attcgttatc aggcggtgca ttccgatgaa gtcgaagaca
    12001 ttctggcgtt ggatatcgct ctgcggcgta acgacaccga gtggtatgag catttaccgc
    12061 cggagatcga cagccagctg gtgcacaagc tctattacgg ccattttatg tgctatgtct
    12121 tccatcagga ttacatagtg aaaaaaggcg tggatgtgca tgcgttaaaa gaacagatgc
    12181 tggaactgct acagcagcgc ggcgcgcaat accctgccga gcataacgtc ggtcatttgt
    12241 ataaagcacc ggagacgttg cagaagttct atcgcgagaa cgatccgacc aacagcatga
    12301 atccggggat cggtaaaacc agtaaacgga aaaactggca ggaagtggag taaaaattac
    12361 ggatggcaga gtatcgccat ccgaattcac ttaatcgttc tgtgccgtct gccccgccgc
    12421 cgccatttgg gcggcttttt gttttttata gctcaacgct gctgccggca caggcatcac
    12481 tttaccggtt tcaatccagg tacgcaggcg gctggcatcg gcaaaatggg tatatttgcc
    12541 aaacgcgtcc atcactacca gcgccaccgg tttattattg ataaccgtac gcatcaccag
    12601 acaatggccc gccgcattgg taaagccggt tttggttaac tgaatattcc agttatcgcg
    12661 atacaccaga tgattagtat tgcggaacgg cagcgtatac gtcgggttag agaaggttgc
    12721 catatcttcc cgggtagtac ttaactgccc gatcaacgga tattgtttgc tggcaatgag
    12781 cagtttggtt aagtcacggg cagttgaaac gttatgcacc gacaatccgg taggttcaac
    12841 aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca ttcattgcct taataaaggc
    12901 tttgtaacca ccgggataat ggtgtgcaag gcttgccgcc gcgcggtttt ctgaagacat
    12961 cagcgccagc aacagcatat ctttacggct gatttcgcta ttcagtcgta cgcgcgaata
    13021 gacccctttc atctccggcg tctggctgat atccactttt agtttttcat ccagcggcag
    13081 tcgtgcatcc agcacaacca tcgcggtcat taatttgctg atagacgcaa tcggacgcac
    13141 cagatccggg tggttcgaat agatcacttt gttggtattc agatcaacaa tcatcgcgct
    13201 accggaggca atttccggtt gtgaagcggt ggtagcggct gccgttttcg caacggcctg
    13261 cggtgcaaaa ggcacagcca gcatcagggc caggctaaat aaagaaactc gaaatttcgg
    13321 catgatgagc attcagatag tggttcacgc gcacgggttg cgcaccgccg gagtaaggat
    13381 ttactgaggc tagcgacgcc atcataacga gcaaaaagtg cgatcgtcaa aggagaatcg
    13441 tgaggaaatg ctgcattgct gacatttacg ccagcaatgc aacgtcagag aactttctag
    13501 aacaaacgat aaccgtagcc ccatagtata acggttaggg cgagcagcac ttccagtacc
    13561 agcacgccaa tcgccagcgt cgaactagag aagctaaggc cttcctcttt gttgatattc
    13621 aggaagctcg gaataccaag gtaaagcagg tagccggtgt aaaacagcgc caccgtgccg
    13681 accagcgcac acaaccagac cagtggataa agcgccacca gaccgcttaa aaacagcgga
    13741 gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg acggacgctg cggataatta
    13801 cgcgccatcc accagatgac ccgccccatc accgcgaccc cagccagcat aacgccgtaa
    13861 aacaagacag ccagcgccag tccggtaaac caggataact tcaggatagt gccatcgcca
    13921 aaattccagc caatctgtgt agtgccaatg aaggcgcaaa tcaccggaat cgccgccatc
    13981 agcaaaacgt ggtgggtgta atggtgagaa atcgtttcgt tttcgcgatt aatcacctgc
    14041 atttcacgat cgggatggga aaacagtccc cagacatggc tcataccgcc cccttgttgt
    14101 gagttcatga acctgacagt tcaagtataa gtcagcttgt gattattttt tgttcgccca
    14161 tgtaattttc actgtctgat gaacgtcctt tttcagcgtg aatgattcac agggtgtatg
    14221 cttactggca accaaaggga gacagactgg cctatggatc tcaatacact tatctcacaa
    14281 tatggttatg ccgcgctggt gatcggtagc ctggcggaag gtgaaaccgt gactttgctg
    14341 ggaggcgttg cggcgcatca ggggctatta aagttcccgc tggtggtact ttctgtggcg
    14401 cttggcggca tgattggcga ccaggtgctc tatctgtgcg ggcggcggtt tggcggcaag
    14461 ctgttacgcc gtttctcgaa acatcaggat aaaattgagc gggcgcagaa acttatccaa
    14521 cgccatccgt atctgtttgt cattggtacg cgctttatgt atggctttcg ggtgattggc
    14581 ccgacgctga ttggtgccag ccagctgccg ccgaaaatct ttctgccgct gaatattctc
    14641 ggcgcatttg cctgggcgtt gatttttacc actattggtt acgctggtgg tcaggtgatt
    14701 gcgccgtggt tgcacaatct cgaccagcat ttgaagcact gggtctggtt gattctggtc
    14761 gtggttctgg tggtgggcgt gcgctggtgg ctgaaacggc gcgggaagaa aaagccggat
    14821 catcaggcgt aaaaccattg ccctgggtaa ggcgttcgcg ccgcatccga catccggtgc
    14881 accatgcctg atgcgacgct tgtgcgtctt atcatgccta ccaaaatttt acccattgaa
    14941 ataaccacgc acctcactgc gctattctgg gttgaactgc ggattcgcca acataaagcc
    15001 gccatccact atcaacgact gcccggtggt gtaatttgcg ccctctgagc acagccacgc
    15061 taccaggctg gcaatctcat gcgttgcgcc aaaacgccgc agaggaatcg aaggctccgc
    15121 gtcgggcttc acgtcgctgt catccatgcc attcattggc gtggcgatcg ccccaggcgc
    15181 gactgcgttc accaaaatct tatgcctgac cagctccagc gccatcgttt tggttaaccc
    15241 gccgagcgca tgtttagcgg ctgtgtaagc gctggcatcc ggcagcggcg tatgttcatg
    15301 taccgacgta atgttgatga tgcgaccgcc ctgcccttgt ttcaccatct ggcgggccgc
    15361 aatttgtgag cataaaaatg caccatcgac atcaacggta aaaatcttgc gccactcatc
    15421 gaaagccata tcaagaaacg gcgctttggt cattgcgccc gcattattca ccagcacatc
    15481 aatacgcccc agccgttgaa tgagtttctc cagcgcctgc gcaccttctg gcagtttacc
    15541 gagatccagc tgcacgatct ccgcacgtac gccgtggcta actacctcac gcgcggtatc
    15601 ttttgcccct tcttcatctg agtgccaggt aatgccaata tcaaacccct gctgcgccag
    15661 taataacgcg cactctttgc cgatccccga atcggaggcg gtaataatcg caacctgtgc
    15721 catcgagttc tccacttaac gctgaataaa cgttaagtat agaaggcgca tatcatcagc
    15781 gtttgtaacc cccgcccaac gcaccagtga gttgaatgga agcatccagc cactgccctt
    15841 gcaataacag gccattggcc cgctcacgca gcgcggggat tctggcttcg ctgacgcggg
    15901 aaccagcaat gatgcccgcg ttaaagcacg cctgcgcaag acctaccaca cgcaaggcat
    15961 cgcgctcaat ttgcgcctga tgctggtttt tctccgcaag tgtctgaacc tgactggctg
    16021 cccgcgccac gtcattcacc gcttcaacca ccgctttgtt gtagctggcg atagacaagt
    16081 tgctttcggc ttttgcgata tcgagattgg cgttaagacg accactatcg aaaatgggta
    16141 gcgtcaggcc tgccgtaacg cccatttgct gcgcggaatg acggaacaga tcgcttaagt
    16201 gcaacgcatc ctgttgcagg aaggccatca ggttgatgtc aggataaaat gccgcttttg
    16261 ccgcatcaat ggtgcttagc gatgactcaa cgtaccagtg cgccgcctgc aaatctgccc
    16321 gccgggccag taaggagtac cccagttcat caggaagctg gcttgccact ttcggcaacg
    16381 cgaccggttt aagcttcaat gactttgtct ggttatttgt aagtgcgctt aaccgtgcct
    16441 caataatttt cattttcccc gcgacatcgt tgagctgctg ccgggttttg ctggcattaa
    16501 tatcggtttc cacaccttca actgaagaag taatcccgtt ctgatatagc tggcgatcgg
    16561 tcgcgataat ggtgttctgc tctttttcta tttgctgcaa gaccgtgttt aacgccgcct
    16621 gggtttgcca ctcccagtac aggcgggcta cgctgccagc cagcaattgg cgggtttgct
    16681 cgcgttccgc cgcccgtgct ttaaccgtac ccaggcgggc agtaacctcc gcccgattct
    16741 ttccccagat atcgagatgc cagcccgccg ttaagccaaa agttccgttg gtgtaccacg
    16801 gaccggtcgt acctgcggcc ggatcgttca gagcaaacgg ccccattaag ccttctgccg
    16861 acattttttg ccgctccata tccgcagaaa agtcgatctg cggaccatcc tgagtagcaa
    16921 ttgccttcgc ctgggcttca gctagctgaa tgcgctgttc agccacctgc atatccggtg
    16981 cgttctgtag tgcattgtta attaaggaag tgagttgatt atcgtgatac tccagccacc
    17041 attggctgtc tggccaacca tttttcagcg ccgtgggtaa tgcggtgtca acttgtgcag
    17101 caggcgtttg ctggcttaac gcctggcggg tttcatgcat aggtgcacac ccggccagca
    17161 tcagtaacag cggaaaacag gcgatggctg gataaaagga atcacgattc atgggggaat
    17221 aatcaggtaa gaaaaggtgc gcggagatta ccgtgtgttg cgatatattt tttagtttcg
    17281 cgtggcaata catcagtagc aataaaacga catatccaga aaaatataga ctaagtaaat
    17341 gatatcttcc gatttatctt aatcgtttat ggataacggc aaagggcttc gttttttcct
    17401 atacttattc agcactcaca aataaaggaa cgccaatgaa aattatactc tgggctgtat
    17461 tgattatttt cctgattggg ctactggtgg tgactggcgt atttaagatg atattttaaa
    17521 attaattaat gtcatcaggt ccgaaaataa cgagaatatt tcagtctctc atcctgttgc
    17581 gctcctgtca tgtgcattgc ttcatataat cactggcgca aggagcgcgc agggcggcca
    17641 atcgccgccg ccccctgcac ccccgggctc tggcgaacaa aatcgccgct gcgcggtgcc
    17701 ctcggcttat cccttacggc taccgggtcg ggcgcgaggt aacatccctg taaaacgcgc
    17761 cctcagccca catccatgtg ggctgcccca gccttcaggg aacgcctcgg caattttgac
    17821 gccaccaaac aaccgtgcgg cctattgata aagagctaac acattgtcaa aaaacatcac
    17881 tatggttttt tagagtttct cgatatcaat tgcctgaata gcccttgcaa tatcagggga
    17941 attattcaac acccgaacat gctgaaataa ttccgttgct tcatcgtatt ctttacgcaa
    18001 ataactcaac cactgtttaa tccgcgcaac gtgatataac ccggtatcgc cctgcttttc
    18061 cagacgggta tatttttgca gcaaagcaac cacctccggc cacggcattc gcggttcgtt
    18121 atattttact acccggctca ggttgggaat attgagcgcc ccgcgaccaa tcatcactgc
    18181 gtcgcagccg ctgatcgcaa tgcattgttg cgcgctctgc cagtcccaga tttcgccgtt
    18241 ggcaatcacc ggaatattca gccgctggcg aatctcgcca atcgcctgcc agtcaatatg
    18301 ctccgcgcgg taaccctgct cttttgtccg tccatgcacc accagctccg tagcgccagc
    18361 ctgttgaacc gcatcggcga tttcaaattt cttctcaccg ctgtcccagc ccagacgcac
    18421 ttttacgctg acgggcaaat gcgctggtac agcttcacgc atcgcttttg caccctggta
    18481 gatgagttca ggatctttga gtaacgtcgc gccgccgccg ctaccgttaa ccgttttcga
    18541 cgggcagccg caattgagat ccacgcccca ggaacctaac tccaccgcac gggcggcgtt
    18601 ctctgccagc cattgtggga actgacctaa caactgcacg cgcaccagcg taccagatgg
    18661 tgtccggctg gcgttttgta gctcagggca aatgcgatga aagactttta ccggcagcag
    18721 ttgatccacc acgcggacaa actcggtgat gcacagatcg tagtcgttaa cttcggtcag
    18781 caattcacgc accagagagt caagcacacc ctccatcgga gccagtaaca cacgcatatc
    18841 ttcacccgca aaaaaatgag gcgccatgtt agcgcctatg gtcaacggat taaaggtctg
    18901 caaagaggga ttgctgcaaa ctgtaatccc tctctatgct tgatgtatgc ctgagttcgt
    18961 tgcgcattgc gtaatgccgg ggagcgaaaa aataagcgct gatttcttaa tgtgatcggt
    19021 agcacgtttt acgaattaat tgtatgatga atccatctca tctggggtgt tgattatgag
    19081 taagacactg aacattatct ggcaatattt acgcgctttc gtcctgattt atgcctgcct
    19141 gtatgcaggc attttcattg cttccctgct accggtaacc attccgggca gcatcatcgg
    19201 tatgctgatc ctgtttgtcc tgctggcctt gcaaattctt ccggcaaaat gggtcaatcc
    19261 ggggtgctac gtactgattc gctatatggc gctattgttt gtgccgattg gcgtaggcgt
    19321 catgcaatat tttgatttgc tccgcgcaca gtttggcccg gtagtggttt cctgtgcagt
    19381 cagtacgctg gtggtttttc tggtggtgag ctggagttca caactggtac acggtgaacg
    19441 taaagtcgta ggtcagaaag gatcagaaga atgatggcga atatctggtg gtcattaccg
    19501 ctgacgttga ttgtcttttt tgccgcccgc aaactggcgg cacggtataa gttccccctg
    19561 cttaacccgt tgctggtagc aatggtggtg atcattcctt ttttaatgct caccggcatc
    19621 tcttacgaca gctactttaa aggtagcgaa gtgctaaacg atttgctgca accggcggtt
    19681 gtcgcgttgg cttatccttt atatgagcag ctacaccaga tccgcgcgcg ctggaaatcg
    19741 atcatcacca tctgttttat cggcagcgtg gttgcgatgg tgacggggac ttccgtggca
    19801 ttgttgatgg gcgcttcacc ggaaatcgcc gcgtcaatcc tgccgaaatc agtcaccacg
    19861 cctattgcaa tggcggttgg cggcagtatt ggcggtattc cggcaatcag cgcagtttgc
    19921 gtgattttcg tcggcatcct cggcgcggta tttggccata cattgcttaa tgcgatgcgt
    19981 attcgtacca aagctgcgcg cggtctggca atggggactg cctcgcacgc cctcggtacg
    20041 gcgcgctgcg ccgagctgga ttatcaggaa ggtgcattta gttcgctagc gctggtgtta
    20101 tgcgggataa ttacttcgct gatcgcaccg ttccttttcc cgattattct ggcagtaatg
    20161 ggctaaaatt tgcgatgcgt cgcgcatttt tgatgtaagt ttcacgcgtt gcataattaa
    20221 tgagattcag atcacatata aagccataac gggttcgtaa actgttatcc cattacatga
    20281 ttatgaggca acgccatgca tccacgtttt caaaccgctt ttgcccaact tgcggataac
    20341 ttgcaatctg cactggaacc tattctggca gacaagtact tccccgcttt gttgaccggg
    20401 gagcaagtct catcgctgaa gagcgcaacg gggctggacg aagacgcgct ggcattcgca
    20461 ctacttccgc tggcggcggc ctgtgcgcgt acgccattgt cgaattttaa tgttggcgca
    20521 attgcgcgcg gtgtgagcgg aacctggtat ttcggtgcca atatggaatt tattggtgcg
    20581 acaatgcagc aaaccgttca tgccgaacaa agcgcgatca gccacgcctg gttgagtggt
    20641 gaaaaagcgc ttgcagccat caccgttaac tacacgcctt gtggtcactg tcgtcagttt
    20701 atgaatgaac tgaacagcgg tctggatctg cgtattcatc tgccgggccg cgaggcacac
    20761 gcgctgcgtg actatctgcc ggatgccttt gggccgaaag atctggagat taaaacgctg
    20821 ctgatggacg agcaggatca cggctatgcg ctgactggtg atgcgctttc tcaggcggcg
    20881 attgcagcgg caaaccgttc gcacatgcct tacagtaagt cgccaagcgg tgtcacgctg
    20941 gaatgtaaag acggtcgtat tttcagcggc agctacgctg aaaacgccgc attcaacccg
    21001 actctgccac cgttgcaggg agcgttaatt ctattgaatc tcaagggtta tgattacccg
    21061 gatatccagc gcgcggttct ggcagaaaaa gccgatgcgc cgttgattca gtgggatgcc
    21121 acctccgcaa cactgaaagc tctcggctgt cacagtatcg accgagtgct tctcgcttaa
    21181 gcctggtgcc ggatgcggcg tgaacgcctt gtccggcttg ccagccctct cctggtgtcg
    21241 aaattcccgg caaacagttt gccgtttctt gcgcaaaacc agcgggtaaa gtagcctgat
    21301 ggaaattttc cttagatcga gtctcctgca tgttaaagcg cgtgttcctc agcctgttag
    21361 tcctgatcgg cttgctgctg ttgactgtgc tcggcctcga tcgctggatg agctggaaaa
    21421 ccgcgcctta tatctacgac gaattgcagg atctccccta ccgccaggtc ggtgtggtgc
    21481 tcggaacagc aaaatattat cgtactggcg taattaatca gtattatcgc taccgcattc
    21541 aaggagcgat taatgcctat aacagcggta aggtaaatta tctattactg agcggcgata
    21601 acgcattgca aagttataat gagccgatga ccatgcgcaa agatttaatc gctgctggtg
    21661 tcgacccatc agatattgtt ctcgattacg caggctttcg tacgctggat tccatcgtgc
    21721 gtacacgcaa agttttcgat actaatgatt tcattattat cacccaacgt ttccactgtg
    21781 agcgggcatt atttattgcg ctgcatatgg ggattcaggc tcagtgttat gccgtaccgt
    21841 caccgaaaga tatgctgtca gtacgtattc gcgaatttgc cgcccgtttc ggtgcgctgg
    21901 ctgaccttta tatttttaaa cgtgaaccgc gttttttagg gccgctggtc cctattccgg
    21961 ctatgcacca ggtgccggaa gatgcgcagg ggtatcccgc cgtcacaccc gaacagttac
    22021 ttgaattaca aaagaaacaa ggaaagtagt tatggatgta cagcagtttt ttgtcgttgc
    22081 cgtttttttc cttattccga tattttgttt ccgcgaagca tggaaaggct ggcgcgcagg
    22141 cgcgattgat aaacgggtta aaaatgcacc ggaaccggtg tatgtctggc gagcaaaaaa
    22201 tcccggactc tttttcgctt atatggtggc atatatcggc ttcggaattt tatctatcgg
    22261 catgattgtt tatcttattt tctatcgtta atattttctc ttttctgtta attattgaga
    22321 ataattatta cttcactcaa taagctgcgg atataacatt ctgaaccgca gctatttgtg
    22381 aatcctttca cagtataaat ttccccacgc gcatagcctt aatatcagca cgcaatattt
    22441 atactctaaa taattcgagt tgcaggaagg cgacaagtga gtgagtcccc aggagcttac
    22501 ataagtaagt gactggggtg agcgaacgca gacgcagcac atgcaacttg aagtatgacg
    22561 agtatattta gcgttcgcct taattactca tagcattaag gaagatcaca tgccgcaaca
    22621 aaattatctg gatgaactca ctccggcttt tacgccttta ctggcgatta aagaagcctc
    22681 tcgctgttta ttatgtcatg acgctccctg tagtcaggct tgcccggcac agaccgatcc
    22741 ggggaaattt attcgctcaa tctactttcg taattttaaa ggcgctgccg agacaattcg
    22801 cgaaaataat gccctcggtg ccgtttgtgc cagagtgtgc ccgacggaga aattatgtca
    22861 aagcggttgt acccgtgccg gtgttgatac tcccattgat atcggtcgct tacagcgttt
    22921 tgttactgat tttgaacaac aaactgggat ggaaatttat cagcccggta ctaaaatgct
    22981 cggcaaagtc gcgattattg gcgcaggtcc tgccggattg caggccagtg taacactgac
    23041 aaaccagggt tatgacgtca cgatttatga gaaagaagcg caccccggtg gctggttgcg
    23101 taacggtatt ccgcaattcc ggttaccgca atcagtgctg gatgcagaga tcgcccgtat
    23161 tgaaaaaatg ggcgtgacca ttaagtgcaa caacgaagtc ggtaacacac tcacccttga
    23221 gcagctgaaa gcagaaaacc gcgcggtact ggtcaccgtg gggttatcga gcggttccgg
    23281 gttatcgctg tttgagcata gcaacgttga gattgccgtg gacttcctgc aacgtgcacg
    23341 acaggcgcaa ggcgatatca gcattccaca aagcgcatta attatcggcg gcggtgatgt
    23401 tgcgatggac gtagccagca cgctgaaagt tctcggctgt caggcggtaa cctgcgtagc
    23461 gcgtgaagag ttagatgagt ttccggcaag cgaaaaagag tttgccagcg cccgggagct
    23521 gggcgtttcg atcatcgatg gattcacgcc agtagccgtg gaaggcaata aagtcacgtt
    23581 taagcatgta cggttaccgg gcgaactgac gatggcggca gataaaatta ttctcgccgt
    23641 cggtcagcat gccagactgg atgcctttgc ggagttagag ccgcagcgta acaccatcaa
    23701 aacacaaaat tatcagaccc gcgatccgca agtctttgct gctggcgata ttgttgaggg
    23761 tgacaaaacc gtggtctatg ccgtgaaaac cgggaaagaa gccgccgagg cgattcatca
    23821 ctatttagag ggagcttgct catgttaacg aaagatcttt cgattacttt ttgcggcgtg
    23881 aagtttccca acccgttctg cctctcttct tcgcctgtag gcaactgcta tgagatgtgt
    23941 gccaaagcct acgacacagg ttggggcggc gtggtgttta aaacgatcgg cttttttatc
    24001 gccaacgaag tctcgccgcg ttttgatcat ctggtgaaag aagataccgg ttttatcggc
    24061 ttcaaaaata tggagcagat tgctgaacat ccgttggaag agaatctggc cgccctgcgt
    24121 cagctgaagg aagattaccc ggacaaagta ttgatcgctt cgagcatggg ggaaaatgag
    24181 cagcaatggg aggagctggc gcgcctggtg caagaagctg gcgcggatat gatcgagtgt
    24241 aacttctcct gtccgcaaat gacttctcat gcgatgggta gcgatgtcgg gcaaagcccg
    24301 gagctggtag aaaaatattg tcgggcagtg aaacggggtt ccacgctgcc aatgctggcg
    24361 aagatgacgc cgaatatcgg tgatatgtgc gaagtggcgc tggcggcgaa gcgcggcggc
    24421 gcagatggca ttgcggcgat taacaccgtt aaatccatca ccaatatcga tcttaatcag
    24481 aaaatcggta tgccgatcgt taacggaaaa tcgagtattt ccggatattc cggtaaagcg
    24541 gtaaaaccga tcgccctgcg cttcattcag caaatgcgca cccatccaga actgcgcgat
    24601 ttcccaatca gcggtatcgg cggcattgaa acctgggagg atgcggctga gtttttattg
    24661 ctcggcgcag caacgttaca ggtgaccacc ggcatcatgc agtacgggta tcggatagtg
    24721 gaagatatgg caagcgggtt gtcgcattat ctcgccgatc agggatttga ttcgctgcag
    24781 gagatggtag gtctggcgaa taacaatatt gtcccggcgg aagatttaga ccgcagttat
    24841 attgtctatc cccgtatcaa tcttgataaa tgtgttggct gtggacgctg ttatatttcc
    24901 tgttacgacg gcggtcacca ggcgatggaa tggagcgaga aaacccgcac accgcattgt
    24961 aataccgaga aatgtgtggg ttgtctgctt tgtggtcacg tctgcccggt gggttgtatt
    25021 gagctcgggg aagtgaagtt taagaaaggc gagaaagaac acccggtaac gttgtaaaac
    25081 cagagacgca tccggcattt ggcaccgatt gccggatgca gcgcatccgg caatatccac
    25141 aatcacatca cttcttacgc gcgtatttca gtgaatccag cgctacggcg aagataataa
    25201 tcgccccttt gatgatgtac tgccagtatg ggtttacgcc gatatacgtc aggccatagt
    25261 tgatgacggt aaaaataatt accccggtca ccacgccaat caccgtcccc acaccgccgc
    25321 tgaacgatac accgcctacc acgcacgccg cgatagcatc cagctcatac ataaagccga
    25381 ggttgttggt ggcagagccg atacgtccgg cttctaacat cccgccaaag gcatagaaca
    25441 cgccagacaa cgcgtagatc atcagcaggt tcaggccgac gttgacacca gatacttttg
    25501 ccgcttccgg gttaccgcca atggcaaaaa tgttcttacc gaagcgggtt ttgttccaca
    25561 acacccagac gaacgccacc gcaatcaacg cgtagaaggt gatgtaagag agacggaaac
    25621 tccccagcgc gacaaagccc tgagcaaagg tagagaagcc actgtcaaaa ccagaaattg
    25681 gcgacgcccc gacaaagtcg taatagagcg agttgatgcc atagacgatg atcatcgtgc
    25741 cgagcgtggt aatgaacggc gtcacgttga gataagcgat aatcagaccg ttgatcaaac
    25801 cgatcaccgc accaatggca cagacaatca gaataaccag cgcaatcggc atcgtcgcca
    25861 tttccgggaa cactttgttg gcgttatcca tggactgcaa taatgtcgca gccaccactg
    25921 ccgccagccc tacctgacga ccagcagaaa gatcggtccc ctgggtgaca attaaccctg
    25981 ccacaccgag cgcgataata atacgcaccg atgactgggt gagaatatta cttaagttca
    26041 acagacttaa aaatgttggg tcctggaaaa taataatcgc cagcaaaact aaaagaacga
    26101 cgtaaatacc gccctctttc aggtaagtaa gaaaactttt cttatttaac gcactcatgg
    26161 gaagccccta atcttaaagg tgcaaagacg caagacgcag aatttcgttt tgcgttgttg
    26221 tttttgtatc gacaattccg gaaacgagac cattgctcat gaccagaata cggtctgtta
    26281 tccctaacaa ctcaggcatt tcagaggaga taataataat ccccttgcct ttcttcgcca
    26341 gttcggcaat taactgataa atttcaaact tcgccccgac atcaataccg cgcgtcggtt
    26401 catcgagcat taatatttct ggttgcgtta gtagccagcg accaataatc accttttgct
    26461 gattaccacc ggagagcgaa ccaatttgcg tccgatgacc cggcgttttt acccgcatcg
    26521 aatcaatcac ccactgggta tcgcttttca tccgcgagtt atccagtaaa ccaactttat
    26581 ttttgtagtt gcgaatattg gaaattaacg agttaaaacc aatatccaga taggcataaa
    26641 ttcccgttga gcggcgctcc tcagttacca gtgcaaatcc gtggtttatg gcttcgttgg
    26701 cattatggtt attgatctgt ttgccgtgca acgtaatggt gccagccgat ttctcgcgaa
    26761 taccaaataa cgtctcaaca atatcggtac gtttcgcccc caccagcccg gcaataccga
    26821 ggatctcccc tttatgcaga tcaaacgaga catcgcgaat cgacggctgg cgcagtgacg
    26881 ttaggttacg cacctcgagg atgacttcgc ccggcttgtt ttctttgtca ggaaaacgct
    26941 gattaagaga acgcccaacc atcatggcga tgatcttgtc catcgtcagt cctgccagcg
    27001 gttcggtggc gatccactga ccgtcgcgca atacggtaac ttcatcacat aactggaaga
    27061 tttcttccat tttatgcgag atataaacaa taccgcagcc gcgctctttt aatttacgaa
    27121 taatagtgaa cagatgattg acctcttttt cggttaacga agaagtcggt tcatccataa
    27181 tcacaatttt cgcgttatag gaaaacgctt tggcgatttc gatcatctgc atttgcgaaa
    27241 cggataatgt gccgacgcgc gcacacggat cgatatcaat atccagttca tcaaaaatcg
    27301 ctttggtttc gcggtacatt ttgtcctgat cgacaaacat gcctttggtg ggatatcgcc
    27361 ccagccacat gttatccatc accgaacgtt gtaataccag gtttaactcc tggtgtacca
    27421 tcgaaatacc attttccagt gcctctttgg cggaatggaa atcgatctct ttaccctgga
    27481 ataaaatggt gccggagtct ttttgataaa taccaaacag gcattttaat aatgtcgatt
    27541 ttcctgcacc gttttccccc attaatgcat ggatagaatg tggccggact tttaaattaa
    27601 cgttatcaag tgccttaaca ccaggaaagg acttgttgat accgctcatt tccaacaagt
    27661 attccccgga agacggagtc gttgagctga ccatataatt ttaccttgtt ggccatacaa
    27721 taagggcgca gtaaacgact gcgcccaatc agtcttattt cttgctgaat tcagccaggt
    27781 tgtctttatc tacgccaaca taaggtacgc ggaccacttt gttgtcgatt ttccagttgg
    27841 tgccatcagc cgcacctttg ccatcggcca ggtttttcgc cagatcaaag gtcgctttcg
    27901 cctggttgtt agcatcgttc agtactgtgc ccgccagtgc accggatttc accagcgcca
    27961 gcgcttctgg cagcgcatcg acgccaaaca ccggaatgct ggacttgttg tgtgctttca
    28021 gtgcttctac cgcgcccatt gccatcgcat cgttgttggc gataaccact tcgattttgt
    28081 tggcgttcgg gccagacagc caggcgtcca tcttatcttt cgcctgagcg gtatcccaca
    28141 tagcggtatc taactgtaac tgttcagttt taatgccttt gtcgttcagc tctttaatca
    28201 cgtaagtggt acgtgcttct gcatccggat ggcccggttc acctttcagc aatacgaatt
    28261 gaatctgacc gtctttgttc agatcccaac cctgattcgc cgcccagtgt ttagcaatca
    28321 aatcgccctg aataatgccg gactctttgg aatcagtgcc aacgtagtag gctttgtcgt
    28381 agctatccag cgccttacga gacggttctt tgttgaagaa aaccaccggc acgttttgcc
    28441 cacgcgcttt ctcaatcacc gtacccgcag ctgccgggtc aaccaggttg attgccagtg
    28501 ccttcacccc tttcgccagc aatacgtcga tctgatcgtt ctgcttggac tggtcattct
    28561 gagaatcatt catcagcagc tgaacatctg gcgcggcttt cgcatcttgc tcaatagcct
    28621 tgcgcactac agacataaag ttatcgtcgt acttatagat tgttacacca atgcgagtat
    28681 cagcagcgtg tgcagcggca ccgaataaca tgctggccat cacagcagac agggttaaca
    28741 ccttcttatt catggtatct ccggtttttc ttatgcaggg tagtgcttga gataaatgct
    28801 cggcggggca gtagagttaa tgaagtgtta ctgaacgccg aagctcactt tttaaaattc
    28861 gttcttccat gcccggtaac gctccagaaa acggctttaa ttgttgttat gacgctatta
    28921 cctcggtgaa agtgattatt cactgttaca tacgggttac aacgttaaaa cggtgcaatc
    28981 atagctatca cattgttaag atactgtgaa atcactcaca gattgaaagc ggttacatcg
    29041 cctgatttgt tgagttagtg atcgccgccg cattctggcg cgttgcgaca gaatgacgac
    29101 gcactaacgt cggcataaaa cagtggctgg cacgaggatc aatattgcct gctgccccct
    29161 gcaaggccag ttcggtggct aatttagcca ttgaagcaat gggataacgc acggtcgtta
    29221 attgcgggtc ggtgtaacgg gcgatgggaa tatcatcgaa accgatgatt gagagatgta
    29281 acggaatcgc aatgccatta tcttttaatg ctgtcagtgc gccagcggcc atattgtcgt
    29341 tataagcaaa tacagcggta agttgtagat tgcgccccag cagttcaacc atcgccgcct
    29401 caccgcccgg catgtccggc gtaccagtgc caatccagct ttccggcgga ataatatcct
    29461 gctctttcaa cgcactcatc cagcctgctt tacgcatggc gtcatcttca ataccgtggc
    29521 tggaagaaag ataaccaata cgttgatgac cgttattcag caacatgcgc gtcgccattc
    29581 gggcaccgct gagattatcc aggcaaacgc aacgatgggc gtaccccggc acaacgcggt
    29641 tgattaacac cataccggga atgttatcca taaattgcgc cagttcatcg tcactcaatg
    29701 cttttgagtg aacaatcaac gcattacaac gctggcgaat taacacctca atggcgtgac
    29761 gctctttttc cgcttcatga tagctattgc cgattagcac gtatttctga tgctgctggg
    29821 cgaccagatc caccgctttt accagcgcgc cgaaaaaagc atcagaaacg tccatcacca
    29881 ccacgccaat ggtatcgcta acctgagttg ccagcgcctg ggcattggca tttggccgat
    29941 aatccagctc actcacggct ttcattactg cttcacgcgt gtcggcactg accagcgtgc
    30001 tgttattgag cacccgggaa accgttgcca cagagacgcc agcctgacgc gctacatcac
    30061 gaatggtgat catattcact acctgttatg agattgcagt aaatgactgc ttgctggcgg
    30121 ctattttgtc agcactgaat acaggacttc gtgaatcgag tcacagcaat ggaaacggtt
    30181 acagccgttt tgttaacgaa tgtgatccag atcgttatct tgatgtttta gatattgccg
    30241 ttccggcagc acgcaaagtt aactgacgcc agagccattc caccggcccc tggcggaagt
    30301 aacgcagcca gataacagag aagaggatat tcgccagcca taccggaata acaaacgcca
    30361 gcagctccag gcgatcaaaa tgcataaaca aaccgaggtg gtaaaaaagc atggtacaaa
    30421 tcagcgtttg caatagatag ttggtcagcg ccatccgtcc gacgcaggcg atcgcaagca
    30481 ccagcttaaa gcggctcaat tgcggccaga agccataaaa cagcgacgca tagccaatcg
    30541 cctgaaacgg cgcactcagt tcccgcggca tttgaagtaa gaaggcacac cagcgatacg
    30601 cccagtccag ctgccattgc agggcgatgg caggaaggtt aatggtcacc ccaatcgcca
    30661 ccagcacaaa accagtacga cgatagtgac gtaagctgaa ctgccctttc agccagccgc
    30721 tgcgcatcag cgccgcacca atgagcatca tccctgccag ttgccagcca tactgtgcgc
    30781 ccagtgccag taaactgttg ccaacaccat cggcacgatt actgatcgct tcaacgccgc
    30841 cgtgaagctt ccagtatttt tcatacaaaa tagccgatgc atccggcgtc caggcgcggc
    30901 tggtctggct gtcggaaatc agccccaata acagcaaaac gccaagcccc accagataaa
    30961 gcatgacgcc tgtattaaac aggcttttta ccgatggcgc atcgcgcacc agccgccagc
    31021 agattaagcc caccagcccg taagccagca gaatatcgcc gtcccagaac aataaaccgt
    31081 gaataaaacc cagcaagact aacagcgtta accgcgactg gatccagcgt ctgccacggg
    31141 gcagcaacat ttgcaggccc gcaccaaaca gcagcgcaaa aagcgtgagg aatttcacct
    31201 ggccgacgag atcaagaaat gcccaggtcc atgcatcctg cggcgtaata gcgccgtacc
    31261 aggcgggatt gagataagcc gcctttggta gcccaaaggc gctgatgttt aatagcagga
    31321 tccccagaat ggcgacgccg cgaacaaaat cgagcgtgac gttgcgctcc atggttcctg
    31381 ccttttaatc agttgtgatg acgcacagcg cgcagaaact cgtggcgcgt attctgactg
    31441 gatttgaaca atccaccaag agaggtcgtt gtcgtggcac tggttgcatc gcggatgcca
    31501 cgcgccttca cgcagtaatg caccgcgtcg atcgagacag ccacgttatt ggtgcccagc
    31561 agcgtttgta gcgcaataag aatttgctgc gtcagacgtt cctgcacctg cggacgctgg
    31621 gcaaagaact gcacgatacg gttaattttt gacagaccga tcaccgaatc tttcgggata
    31681 taagccaccg tcgctttgcc atcgatggta acaaaatggt gttcacaggt actggtcaga
    31741 gtgatatcgc gcacggtgac catttcatcg accttcattt tgttttcaat gagggtgatt
    31801 ttcgggaagt tggcgtaatc cagaccggag aaaatttcat cgacatacat tttagcgatg
    31861 cgatgcggcg tttccatcaa actgtcatca gccaggtcga gattcagcag ctgcatgatt
    31921 tcggtcatat gaccagcaat aaggcttttg cgcgtttcgt tatccatttc atgcacgggc
    31981 gggcgcagcg gtgtttccag tcctcgcgca actaacgctt catgaaccag ggccgcttct
    32041 ttactgagtg atggcattta tgatttctcc tgcaggtgtg acgcctccgc cctgcgtggg
    32101 ggcaaagtta ttaagctgat ttacagcctg attattgtgc gtgaggcggc gcacataatc
    32161 cagtattcac agcgataatt attgtaattg ccgctgcctt tcatcagcag atgttaaaac
    32221 atcgttatgc aaatacggaa gtgaaagtta ctcacagcac attgaataaa cggtatgatg
    32281 aagaaattgc aaacaacaca acaaggagcc acgcatggaa atgctcgaag agcaccgctg
    32341 ttttgaaggc tggcagcaac gctggcgaca cgactccagt accttaaact gcccgatgac
    32401 gttcagtatc tttctcccac cacctcgtga tcacacccca ccaccagtgc tgtactggct
    32461 ttccggatta acctgcaatg acgagaactt caccaccaag gcgggtgccc agcgggtagc
    32521 ggcggaactg gggattgtac tggtgatgcc agacaccagc ccgcgcggcg aacaggttgc
    32581 caacgacgat ggctacgatt taggccaggg cgcaggcttt tatcttaatg ccacgcaacc
    32641 gccgtgggcg acgcattacc ggatgtatga ttatctgcgc gatgaattac cggcgctggt
    32701 tcagtcgcaa tttaatgtca gcgaccgctg cgccattagc ggtcattcaa tgggtggtca
    32761 cggtgcgctg attatggcgc tgaaaaaccc cggcaaatac accagcgttt ccgcctttgc
    32821 gcccattgtg aatccgtgca gcgtcccgtg ggggatcaaa gcgtttagcc gctatttagg
    32881 tgaggacaaa aatgcatggc tggaatggga cagttgcgcg ctgatgtatg ccagtaacgc
    32941 gcaggatgcg atcccgacgc ttatcgatca aggtgataat gatcagtttc ttgccgatca
    33001 gttgcaaccc gcagttctgg cagaagccgc gcgccagaaa gcgtggccga tgacgctgcg
    33061 tattcagccg ggatatgatc acagttacta cttcatcgcc tcttttatag aggatcacct
    33121 gcgcttccat gcgcagtatt tactgaagtg aaagtccgcc cggttcgccg ggcatcttct
    33181 catcagaagc gataatccac tgccataaag taacgacgtc cgtcttcgtt atagctgtag
    33241 tcatcacggc tgagatcctt gtcgccaagg ttcagcacgc ctgcgcgcag tttgacgtct
    33301 ttagtcacct gccaggccgc gccggtattc cagatggtat aaccgccagg tgttttagcc
    33361 gtcgcgctgt cggctcgttt ctgcccggta tagtgaccag aaacatagaa tgaccagtct
    33421 tccagcgcca gcggtttcca gtccagcgta ccgttagcgg tatggaacgg cagatcggat
    33481 agcggtttgt tttcgccgtt gctgacatca cgaccatcgt tgtaggtgta gttgaatgac
    33541 agtttccatt catcgttgaa cggaattttc agttcggttt ccacgccctg aatacgagct
    33601 ttgttaacgt tgtagtagct aaataccggt atgcgccgtc cgttagcgcc cgtctcaaaa
    33661 ccgacaaagt tttggtagcc cggtgcagcg ttgacgtcag acgtacggct gatgctgata
    33721 cgatctttca catcgttacg gaaaacggta acgctggatt caaccccttc cagccagcct
    33781 tcttcaccca tgtagtaaag ccccagctcc caactttcgc tggtttctgg tttcagatcc
    33841 gggctaccca caatcttaca tgcgccacgg caggaattgc tcgtccagtc agggctaagt
    33901 tgcaacagag aaggtgcttt aaatgccgtc gcccagcccc ctttcaccgt tacggtgtcg
    33961 gtggcgttat aaaccaggta ggcacgcgga ctccagtgtt caccgtaggt ttcgtgatcg
    34021 tccatacgca cgccggtcgt cagcgccagc ggctcgaaga tccgccattc atcttccaca
    34081 aacagcgcgt actggctggc agacgttttg gagctggttc ccccggtcag gttcaccgca
    34141 tcgctaagtt tgtcgtgacg ccattcaccg ccaaccgtga gaaactgatt aatcgccgtc
    34201 agcggcaacg tgtatttgcc gtcgaccgta ttgctttcgg aagttatcgg gctgctgttg
    34261 ccagggtttt tgttctcgac tttctcaccg tagtatttca gttcgctggt gccgtaatcc
    34321 caacgcccat tatggctgac ggagtagttc tggcgttcca ggcggttttt gtccagcgag
    34381 tcggaatcac gatcctgacg gtcgaaaccg tatccggcag taaaatcgtg attttgattc
    34441 ggtgtccagg caaattcgac attaccgtcg cggctggaga atccttcaat acgcggcgtt
    34501 tctccggtat cggtggtcgt tgagttttgc gggtcatcct tttcacgttt tgccaggctg
    34561 ccgtaagctt tcattcccag cacgccgtca attaatggtc cgctggtaaa gaactgaccg
    34621 ttataggtat caccgcgatc gcgatgttcc tgaatggtgg tatcgacggt aacggtaccc
    34681 gaccatttct gaccgatttt tttggtgatg atattcacta caccgccgag cgcatcggag
    34741 ccgtacagcg acgacatcgg gccacgaacc acttcaatac gttcgatgga atcgaccggg
    34801 atccagttca gatcgaaatc attgtggcgg aagacggcat tgcgggagtt aacgcgttta
    34861 ccgtcgacaa gaatcagggt gtagctgctg tccagaccac gaatactaac gcccttacgg
    34921 ttatcccctt cgttcgtcag ttgtacgcca ggcacttctt tgaggacatc cttcagattc
    34981 tgtaccggtt ttcgctgcag gtcttcctgg gtaatgacgc tgatactggc tggtgcatct
    35041 ttaagatttt gttccacgga agatgcagtg acaaccatcg tttcgccatc atcatcgacc
    35101 gctaacacag gccatgcaca agaaatagcg gacaaacaca gcccgacccg tacgaaaggg
    35161 ttcaacctaa acattccata tctccatgag gtaactacga aaataaaatg ggttatcgct
    35221 cacatcttct tcacgtcccc ttgcgtacgg cagcatcgcg gtggacttat tttttatgca
    35281 ggtgatcatc ccagaaagcc ggaacagcct ctgatttgtc agcctttttt gattgtggcg
    35341 taacgataat gcaaacgata acaattatca atccaaatgt taaattttat atccgcatga
    35401 tttgtcaggg aaatggaatt gaaaaagccc tctcggttga gagggcttag caaggaaggg
    35461 aggaaactta tttcttatcg ttctgcggga acttcatttc gctgtagcgt acgaagtgag
    35521 ttcctttaat cagcttgtag ccgaaccaaa taatcaggaa cagcgggata ccaatatacg
    35581 ttgccgctac gccgccccag tcaatggtat ctttcaggaa cgcttcgtag ttctggccca
    35641 aagtgataat cagacacaga atgaatgcga agatcggccc cagtgggaag aaacctgaac
    35701 ggtacggcag atcgttaatg tcgtgtccct gcaatacgta accgcgacgg aagcgatagt
    35761 ggctaatggc aatccccagc caggcgataa aacccgtcat cccggaggtg ttcagcagcc
    35821 acaggtatac cgtctggttg ccaaacatgg aggtcaggaa gcacagaccg gcaatcaccg
    35881 tcgtcgcata cagcgcatta cgcggcacgc caccacgcga cagtttagcg aaaatgcgcg
    35941 gcgctttacc gtcacacgcc agggtgtaca gcatacgagt agacgcatac ataccggagt
    36001 taccggctga cagcaccgcc gtcagaataa ctgcgttcat caccgccgcc gcagagagca
    36061 gacccgcgtg ctggaacacc agggtgaacg gactaacgct gatgtcttta acatcgttac
    36121 gcagcaggct cggatcggtg tacggaataa tcaggctgat aatcaggatc gcgaacacat
    36181 agaacaacag gattcgccag aacacctgac gtaccgcgcg tggaatgttt ttcgccggat
    36241 cttcggactc gcctgcagca ataccgatca gctcggttcc ctggaaagag aagccgacaa
    36301 tcatagctac gccgatcatc gccgcaaaac caccagcaaa cggcgcttcg ccgattgtcc
    36361 agttgctcca gcccgcaggc tgcgcgcctt tgaagatacc gataatcatc agcacgccaa
    36421 cgatgataaa gacaataact gtcgtgactt tgatcagtga gaaccagtat tccgcttcac
    36481 caaagccacg aactgagatg tagttcagca ggaagataac gccgaggaac aacgcactcc
    36541 agatccagcc cggtgtatcc gggaaccacc agctcatgac cagctgagct gcaaccaggt
    36601 caacggcgat agtcaccgcc cagttgtacc agtagttcca gcccagcgcg aagccaaagc
    36661 cttcttcaac atagttctga ccgtaagtgg caaacgaacc ggaaaccggc atatatgcag
    36721 ccagttcacc gagactggtc atcaggaagt aaaccatcag gccaatcagc atatacgaga
    36781 gcaatgcccc gcccggacct gcctgagaaa tcgttgcgcc agaggcaaca aaaagacctg
    36841 taccgatgga accgccaatg gcaatcatcg tcaggtgacg cgcctttaat tcacggcgta
    36901 agcccggcgc ttctgtggtt ttagtttcgg aaaccataca aaaatgctat ccatcttaaa
    36961 aatgaggcgc gattgtagca gacgattggc aatccttccg gcagaaatac acggttataa
    37021 gacaccttca tgatcgccca gggattataa gtaaagcagc caatctcttt tctggcgaat
    37081 gcatgctgag ggcgctgcgt cacgaaatcg acacagcacc agcatgttct tgtacagcac
    37141 cttaacgcgg tacattcgcg ggatcgcaat agtccagaaa gcgccgtagc gcgttggaaa
    37201 ggtgtttttg ccgatgatgt atacgccaca acgtacgcat caggcgcggg agagggaccg
    37261 caacttcact taatgtgcct gcctgcaatt gatcttcaat cacacgtcgc gacaggcaac
    37321 taattcccaa cccatgacgc accgcatgtt tgattgcctc ggagttacct aattccatcg
    37381 ccatctcaaa cttcggtaaa tgtgacagca atagataatc gacaatctcc cgcgtgccgg
    37441 aaccgcgttc acgcaggatc cacggcgcag cggccagctg ctctaaggtg accggaccac
    37501 gggccaacgg cgaagtcggc gcggcgaaaa ccaccagctc gtcttccagc cacggttcag
    37561 aaatgatttc agtgctgtgg cacggtcctt caataaagcc aatatcaacg cggaaatcca
    37621 gcaccgcttg catcacatcc tggctattcc caacgctaag ttcaatcggc aactgcggat
    37681 aatcatggcg ataacgggcg ataactgcag gcagaatgta gttaccgatg gtactactgg
    37741 catagatacg aatcgcgccg ttgtcttcgc gaaacagttg ttcgatttca accgcctgtt
    37801 caagcagtgc caacgctcgc ggatagagca gccgcccgtg ttcattaaca accagtcttt
    37861 tccccacgcg atcaaacagt tgcacgccaa gctgcccttc caggtcggtc aaggctgcgc
    37921 tcactgctga ttgcgacaac gccagcatca ccgacgcctg ggtggttgat ccacttttca
    37981 atacttctgc aaaaacttcc aactgccgga gggtgatgtg catagtcgct taccacttat
    38041 aaagattaat tataaatata taatcaattt tatttttaaa ccagttagtc gtaaccttat
    38101 gcccggttaa aggagagggt tatgacgaat atcaccttac agaaacaaca tcgtacactg
    38161 tggcatttta ttccggggtt agccctgagt gcagttatca ccggggtcgc cctgtggggt
    38221 ggttccattc ccgcggttgc gggtgccggg tttagtgccc tcaccctcgc aatcttgttg
    38281 gggatggttt taggcaacac catttacccg cacatctgga aaagctgtga cggtggtgtg
    38341 ctgtttgcca agcaatattt attacgtctg ggtatcattc tttatggctt ccgtctgacg
    38401 ttctcgcaaa ttgccgatgt cggtatcagt gggatcatca ttgacgtgct gacgctgtcc
    38461 agtaccttcc tgctggcttg cttcctgggg cagaaagtgt ttggtctgga taagcacacc
    38521 agctggttga tcggtgccgg tagcagtatc tgtggtgctg ccgcggtact ggcgactgag
    38581 ccggtagtga aagcggaagc cagtaaagta accgtggctg ttgcaaccgt tgttatcttc
    38641 gggaccgtcg cgattttcct ctacccggcg atatatccgc tgatgtctca atggtttagt
    38701 ccggaaactt tcggtatcta cattggttct actgtgcacg aagtggcgca ggtggtggcg
    38761 gcaggtcatg ccatcagccc ggatgcggaa aacgcagcag ttatttccaa aatgctgcgc
    38821 gtgatgatgc tggctccgtt cctcatcctg ctggcggcgc gtgttaaaca gctgtctggg
    38881 gcgaacagcg gcgagaaaag caaaatcact attccgtggt ttgccatctt gttcatcgta
    38941 gttgccatct ttaactcgtt ccacctgtta ccgcagagcg tggtgaacat gctggtaacg
    39001 ctggatacct tcctgctggc aatggcgatg gcggcgctgg gtctgaccac tcacgtcagc
    39061 gcgctgaaaa aagccggggc gaaacctctg ctgatggcac tggtactgtt tgcctggctg
    39121 attgttggtg gtggtgcgat taactatgtg attcaaagcg tcatcgcata aaccactaca
    39181 tcttgctcct gttaacccgc tatcattacc gttttcctcc agcgggttta acaggagtcc
    39241 tcgcatgaaa tacattggag cgcacgttag tgctgctggc ggtctggcaa atgccgcaat
    39301 tcgcgccgcc gaaatcgacg caaccgcgtt tgccttgttc accaaaaacc aacgtcagtg
    39361 gcgtgccgca ccgctcacga cgcaaaccat cgatgaattc aaagccgcct gtgaaaaata
    39421 tcactacaca tcggcgcaaa ttcttcccca cgacagttat ctgattaacc tcggacatcc
    39481 ggtcactgaa gctctggaaa aatcgcgcga tgcctttata gatgaaatgc agcgttgcga
    39541 acaactaggg ctttctttgc tcaacttcca ccctggcagc catctgatgc agatttcaga
    39601 agaggattgc cttgcgcgta ttgccgaatc catcaacatt gcgctggata aaactcaagg
    39661 tgtgaccgcg gtgattgaaa acaccgccgg tcagggcagt aacttagggt ttaaattcga
    39721 acatctcgcg gcgattatcg acggcgtgga agataaatcc cgtgtcggcg tctgcattga
    39781 tacctgccat gctttcgctg ccgggtatga tttgcgtact ccagccgaat gcgagaaaac
    39841 gttcgctgat tttgcccgta ttgtcggctt taagtatctg cgcgggatgc accttaacga
    39901 tgcgaaaagc acctttggca gccgcgttga ccgccatcat agcctcggtg aaggcaatat
    39961 cggtcatgat gcgttccgct ggatcatgca ggacgaccgt ttcgacggca ttccgctgat
    40021 ccttgaaacc atcaacccgg atatctgggc agaagagatc gcctggctga aagcgcaaca
    40081 aactgaaaaa gcggtagcct gaagatgaat aaccgggaaa aggagatcct tgcaatttta
    40141 cggcgtaacc cgctgattca gcagaacgaa attgcggaca tgctgcaaat cagtcgttcg
    40201 cgcgttgcgg cgcatattat ggatttaatg cgcaaagggc ggattaaagg caaaggttac
    40261 attctcaccg agcaggaata ctgcgtagtg gtggggacaa tcaatatgga tattcgcggg
    40321 atggcggata tccattaccc gcaagcggct tctcatcccg gtacaattca ttgctcggca
    40381 ggcggcgttg gacgcaatat cgcccacaat ctggcgctgt taggccgcga cgtccatttg
    40441 ctttcagtga ttggcgatga cttttatggc gaaatgctct tggaagaaac gcgccgcgcc
    40501 ggcgtgaatg tctccggctg cgttcgttta catggtcaaa gcacatcgac gtatctggca
    40561 attgccaatc gagacgatga aaccgtgctg gcgattaacg atacccatct gctggaacag
    40621 ttatcgccgc aattattgaa cgggtcgcgc gatttacttc gtcatgcggg cgtggtactg
    40681 gctgattgca acctgacagc cgaggcgctg gaatgggtct ttaccctcgc tggtgagatc
    40741 ccggtgtttg tcgataccgt ttcagaattc aaagcgggca aaatcaaaca ctggctggcg
    40801 catattcaca ccctgaaacc cactttaccg gagctggaaa ttttatgggg acaggcgatc
    40861 accagcgatg ctgaccgtaa tgctgcagtg aatgcgttgc atcagcaagg tgttcagcaa
    40921 ctgtttgttt atttgcccga tgagtcagtt tattgcagcg aaaaggatgg agaacaattt
    40981 ttgctgaccg caccagcgca tacgacagta gacagttttg gtgctgacga tggttttatg
    41041 gcgggcctgg tatatagctt tctggaagga aacaatttcc gtgacagcgc ccgttttgcg
    41101 atggcctgcg cggcaatttc acgcgccagc ggcagcttaa acaaccctac cctgtctgcc
    41161 gataacgcgc tttcattagt gccaatggtg taacaatgtt gccggattcg acgcttgacc
    41221 gcatcttatc cggctcataa atcgcacact acgctaaacc aataaagaac ccggcaatgg
    41281 tggcactcat caggttagaa agtgtcgccg ccgccagcgc gcgtaaacca agctgggcga
    41341 tttccggcgc acggtgtggc gcaaccgcag aaaacgcccc caccaccacc ccgatagaac
    41401 caaagttagc gaaaccgcac aacgcgaagg aaataatcgc cacggtttta gcatccagag
    41461 tgccagccgt ttgcagatag ggtgagaaat tgagataagc gacaaattca tttattgcca
    41521 gtttctgtcc aatcaaactc ccggcaagat tcgcatcagt ccagtcaaca cccattaccc
    41581 acgccagtgg ggccagcagg taacctaaaa tggactccag cgaggcatgt tcaaaaccaa
    41641 accagccgcc aacgccgccg ataataccgt taattaacgc aatgatggca acaaatgcca
    41701 tcaccactgt tgccacaccc gcagcgattt tcagcccggt catcgcccct gtcgcggcgg
    41761 cttcaataat gctttttggc ggtgtttcgg tgaaagagag gttattaaaa gaaacctgcg
    41821 aagattccgt agccgggctt aacaggcggg caaacaagat cccgcctggg atcgccatta
    41881 acgatgccgc cagcaaatat tccacaggta cgcccagtgc ggcgtaacca atcattgtcg
    41941 aaccggcaat tgaggccatg ccactacaaa tcgctgtaaa taattcattg cgattcagcc
    42001 gatcgataaa gggtttgacg attgccggaa tttcgttttg cccgaggaaa atggtagtga
    42061 ccgcgacgaa tgactcgatc ttgctgatat ttaatgcttt ctggaagata ccgccgagaa
    42121 tgcgaattaa gatccccatc acaccgatgt aatagagaat actcaccagc gcggtgacga
    42181 agataattgc cggtaatact ctgaaaccaa agataaatcc tgcgccatca aacagcgtgt
    42241 ccattttcgg cccgaccagc gaaccgaaga taaatgcgct acccgcgtcg ctgtacgcca
    42301 tcactttatg cacgccaaaa gcgacttttt cagcgaccca acgccctggc ggtaaccaaa
    42361 gcataatgcc gccaatcaca acctgtaaca ctaacgccgc gccaacggta cgcaggctga
    42421 tcttcttctt gtttactgac agcaaaaatg caattgccag caataccacc attcccagaa
    42481 cacttctcat gacatccata gtgattatct cttcatgtca tattgggcga cggctttctg
    42541 cccctcagaa atagccaggc ctgcatcttg cgataccggc ccggcttgtt atcaacactt
    42601 aatgggtttt gatgtagccg cgcacgcact cttcgactaa gccccagaac cagtcggtat
    42661 caatagtgat gccgactttg gtattagcgg gcttgccaag aacgcccagc tcgtcgcaga
    42721 cggtacgccc ataacaaggg ccactgttga cgtccacttc gacgtacatc tcctgggttt
    42781 taatgccatc agggttaatc agataaccga tgcaggtggc gtcgtgcacc gggccgccag
    42841 ccaggccgta gttttcgaac tgcgttttga gagtgaagtt catgatgtcg ctgaacagct
    42901 ctccggcagg gccgcctgcc ctttccatcc gagcaattac gtccggggtg caaacggtct
    42961 ggttggtaag atcgaggccc atcatcacta atggaacgcc agaggtgaac accacgcgtg
    43021 cggcttccgg gtcagcaaag atgttgaact cggcagatgg cgtgaagttg cctgtaccgt
    43081 aagcgccgcc cattagcaca atttcacgga ttttgggcag gatcgcgggt tgcatacgca
    43141 ttgccaccgc gatatttgaa agcggaccaa ccggcaccag agtgatatcg ccatcgcttg
    43201 ccatcagggt atcaatgatg tatttcaccg cgtgggtact ttctgcctgg cgggtcagcg
    43261 gctcgaatac cgggccatcc agtccggttt caccgtgaat attatcggca acgatttgtt
    43321 gacgcataat gggctgcggc atccccgcat aaaccggaac attaatctcc agtttctggc
    43381 aaacattcag gccattaatt aatgttttat caagcgtctg attacccgct acaatagtga
    43441 tgcctaataa atctattgcc ggatgtttcg ctgccatcat cattgcaata gcatcatcat
    43501 gacccggatc acaatccaga ataatttttc tcttttccat tgtttatttc ctctgtttcc
    43561 agttgcgtta ttttttctac agcaaagaaa attcgaccgg gccgatgatt gaatcttaac
    43621 aacagcgtac gtatgctaaa tatgagaaat ctcatagcgg ataaacatcg tgaaagaaat
    43681 ccacaataat gatcttaagc tgcaattgat gagtgaatcc gcgtttaagg attgcttttt
    43741 aacggatgtt tcagccgata cgcggctgtt tcatttttta gcgcgtgact acattgtgca
    43801 ggaagggcaa cagccgtcct ggctgtttta cctgacgcga ggccgcgcca ggctttacgc
    43861 cacgctagct aatggtcgcg tgtcgctgat cgatttcttt gccgccccct gttttattgg
    43921 cgagattgag ttaatcgata aagaccatga accacgtgca gtacaggcga ttgaagagtg
    43981 ttggtgcctt gcgctcccta tgaaacatta ccgtccgctg ttattaaacg acacgctatt
    44041 tttacgaaaa ctttgcgtca ccttaagtca taaaaattat cgtaatattg tttctttaac
    44101 tcagaatcaa tcatttccgt tagtgaatcg cctggcagca tttatattac tctcgcaaga
    44161 aggtgatctt tatcacgaaa agcatacgca agcggcagag tatttaggcg tttcttatcg
    44221 acatctttta tatgttctcg cgcaattcat tcacgacggt ttattaatta aaagcaagaa
    44281 aggatatcta attaaaaaca gaaagcagtt gtcaggactg gcgctggaga tggacccgga
    44341 gaataaattc tccgggatga tgcagtaaaa aaataatctg gaatatactc taaataattc
    44401 gagttgcagg aaggcggcaa gcgagtgaat ccccaggagc ttacataagt aagtgactgg
    44461 ggtgagcgaa cgcagccgca gcacatgcaa cttgaagtat gacgagtata gtacgcgatt
    44521 gccggatgca acgcttaata agcgagtaca tccggcaatg aaactcaagc taaaccaata
    44581 aagaacccgg caatggtcgc gctcatcagg ttggaaagcg tcgccgccgc cagcgcccgt
    44641 aaaccaagct gggcgatttc cggcgcgcgt tttggcgaaa tagccgaaaa cgctccaacg
    44701 acaacaccga tagaaccaaa gtttgcaaac ccacagaggg cgaacgagat aatggcaatg
    44761 gtcttgacat ccagcgtgcc gctcgtttgc agatagggcg agaaattgag ataagcaaca
    44821 aactcattaa tcgccagttt ctgcccaatc aaacttccgg caagatttgc atcactccag
    44881 tccaccccca taatccatgc caacggggcc agcacatagc cgaaaatgct ttccagagag
    44941 acattggcaa aaccaaacca gccgccaact ccaccaataa tgccgttgat cagcgcaatg
    45001 atcgccacaa atgccattac caccgtcgcc acaccggcgg cgattttcag cccggtcatt
    45061 gcaccgttcg ccgcagcttc aataatgctt tttggcggtg tttcgctaaa cgacagattt
    45121 tcaaacgtaa cttgcgaagg ttccgtcgcc ggactaagaa tacgtgcaaa caggataccg
    45181 ccaggaatag ccatcagcga agccgctaac aggtagtcaa ttggtacgcc cattccggca
    45241 taaccaatca tcatcgaacc agcaatggac gccatcccgc tacaaattgc ggtaaacaac
    45301 tcgttgcgat tcatgcgatc gataaacggt ttaacgatcg ccgggatctc attttgcccg
    45361 aggaaaatag ttgtgactgc gacaaaagat tcgattttgc tgatgttaag agctttctgg
    45421 aaaatgctgc caaggatgcg aatcagcagc cccatcacgc caatgtagta cagcagactg
    45481 atgagcgcag taacgaaaat aatcgccgga agtacgcgaa aggcgaagat aaaacccgca
    45541 ccgtcaaaca ggacatccat tttcggccca accagcgaac caaaaatgaa ggcgctaccg
    45601 gcatcactgt aagacatcac tttatgaacg cctaatgccg cctgttctac tgcccacttt
    45661 cccggtggga agtagagcat gatgccacca atagcgattt gcagcagcag tgcggctcca
    45721 accgtgcgca aactgatgct ctttttattc actgacaaca gaaatgctat tgccagtaac
    45781 accaccatcc ccacaacact tctcattata tccataatga ttttcccttc atgccagtaa
    45841 acccggcgtc agcgccaggt tttggtatgc ttgatgagta cgggcgacgg ctttctgccc
    45901 gtcagaaatt aacccgcgag acgctggtat tctttggcaa tttcactcgc cagaatggcg
    45961 ttgttgaaca ccagctggat attggatttc aggctgtcac cgccggtcag ctcagcaacg
    46021 cgcgccagca ggaacggtgt actttcttta ccaataacgc cctgcgcttc agcttcagct
    46081 accgcctgat cgatcgccgc attgatagtg tgttccggca tcgcaaactg ttccgggatc
    46141 gggttcgcta ccacgaggcc accgttcagc ccgctttgcc acttcaccgc cattgcacgg
    46201 gcaatttcac tggcgctgtc gagacgaatg ctgacgtcaa acgggctggt acggcagaaa
    46261 aacgcaggca gcgcttttgt ctgatagcca attaacggca caccgaaggt ttctaaatat
    46321 tctgttgtta atcctaaatc gagaatagat ttcgccccgg cacaaacaac ggtgacatta
    46381 gtatttgcca gttcttgcaa atcggcagaa atatcgaagg tatgttccgc cccgcgatgc
    46441 acaccaccaa ttcccccggt ggcaaatact ttaattccgg caagcgccgc aataatcatc
    46501 gttgaagcca cagtggttgc gccatttttt ccggcggcaa caacaaaagg taaatcgcga
    46561 cgactaactt tggtcacgtt atgcccttca cgacccagta attcaatttc ttctttgctt
    46621 aaacccactt tcatcacgcc gccaataatg gcgatagtgg caggtactgc gccctgttta
    46681 cgaatagttt cttctacttc aattgcggtc tgggcatttt gtgggaacgg catcccgtga
    46741 gaaataatgg tcgattccag cgccacaacc ggttttttgt tttttaaagc gtcctgcact
    46801 tccggggaaa tttgtaataa ttcaggggaa atttttaatt cagacattct gcgttctcca
    46861 ctaacgatat aacgttggca atcgataaat cggggttatt ggtgtattca caggagagcg
    46921 ccattgacga acatccctgt gcgaaacgaa cagattcggc aaacggcatt ccgtctaccc
    46981 aacacgaagc aagtcccgcc atcatggcat cgcccgctcc ggtaacatta ataacattgg
    47041 ttttgatcgg cgcagaccag ccattttcac cgctgatatc gctgtaataa acgccgtcgc
    47101 cgcccatgct caataccagt cggttcaggc catgttgatg gaaccaggca gcaacttttg
    47161 ccacatcgtc acgccctgac agcgcaatcc cactcagggt ttccgcttca aggcggtttg
    47221 gcttgagagt gtggatctga tttagacggt cgcgcacttt gacacatttc cacgctgaaa
    47281 ccggatcgac aaatacgggt acgttggcgg cattatccag aatccatgcc agtgcctctt
    47341 cactgatatt acagtctgcg acaatgacct ttgccctctg aataaattca cggtgctgtg
    47401 cgagatattc agctgtaata gcgttgctaa tattcatgtc atttatagca accagcattt
    47461 caccggtatt atcgagtaat gataaatagc ttgacgtatt ttctcctggc acaatcaggc
    47521 atttatcgac ataaacgcca gattgattgg tttgcgttag cagcgattga ccataaaagt
    47581 cactgcctac ggcgctcagt agccaggctt tgttccccag caacgccagg ttttgtgcaa
    47641 tattgcgccc gactccacca ggcgtaaatt ttattttacc tggatttgaa tccgcataat
    47701 ttaatgattc atgtgaatat ccggcgacat caatattcgc cgagcctata attacgacat
    47761 aatccttttc gcgcatagca cgtcctctgg taaaaaatta ccaaccctgt caatccacaa
    47821 gaatatagat actgcaaaga cggtttaaac atgtgttcac atttaaacat gtgctcatag
    47881 tatgcatgtt ttacgtaaag taaattaccg ccagagaggc taaatgggca gatttgagca
    47941 ggctcacaaa atgcgattaa atgcctgaat tacaaaagcg aggaatttgt gacgggatgc
    48001 acagaacagg aacaataaaa aagggcagaa aataatctgc ccttgaggaa ataaccttag
    48061 tgtttgatga acgtttcaag tttctactga aagataaaac catttaaatt cataaaatta
    48121 cattcacaag ctggtcattc cctttgcgct catccaatga tacagggcag gaaatttctg
    48181 ccctgtaaca caccttttat tacgctgctt tcgctactgc gtccacttcc ggacgtttca
    48241 ggaaggcata ggccaaaccc gccaccagcg taccggcaat aattgctacc aggtaaccca
    48301 gtaccggcgt aatagcgcca gggatcagca gaacaaacag accaccgtgt ggtgccatca
    48361 gtttcgcacc aatcgccatt gagattgcgc cagtcagcgc accacccacg atacagcacg
    48421 gtagcacacg catcggatca cgagcagcaa acggaattgc accttccgaa atgaagcaca
    48481 gtcccaatac cagagcggct ttgccacctt cctgctgcgc tttgtcgaat ttgcgacgcg
    48541 ccaccattgt tgccagaccc attgccagcg gtggcaccat acctgccgcc ataatcgccg
    48601 ccatcgggcc ataggtttga gtactcagca gacccacacc gaatgcgtac gctgctttgt
    48661 ttaccggacc gcccatgtca gtacacatca tgccaccgag gatcgccccc agcagaaccg
    48721 cattcgcagt ccccatggtc tgcagccagt gagtcagccc ttcgagaatg ccagcaaccg
    48781 gtttaccgat caggtagatc atcgccagac cgaccaccag actggaaatt agcggaatga
    48841 tcaggattgg tttcagcgcc tccatactct gtggcagttt cagttgcgta ctgattaact
    48901 tcgcaatgta accagccagg aagcccgcaa taataccgcc aatgaagcca gaaccggtgc
    48961 tgacagccag cataccgcca atcagccccg gcgtgaggcc cggacgatcg gcaatggaga
    49021 aggcaatata acctgccagt accggcacca tcagcgcaaa ggctgaacca ccgccaatct
    49081 gcatcagcgc cgctgccaac gtacccggct ctttaaacgc ttcgatacca aaagcaaaag
    49141 aaagcgcgat acacagacca cctgcaacaa ccatcggcag catgtaagag acgcccgtca
    49201 gcaagtgacg gtaagcgcct gcactctctt tcttaccttc agtggtcgct gtttgagctt
    49261 tgcccgctgg ttcatacggc gttgcttcag caaccgcttt atccagttcc tgcgcggttt
    49321 tcttcagcgc cagaccggta gaggtacgat acatcggttt accagcaaat ttcgccagat
    49381 ccacttcgat atctgccgcc acaatcacca gatccgctgc ggctacttcc tcgggagtga
    49441 ttgcattacc cgcgccaaca gaaccacggg tttcaacttt cacccaccag ccacgttttt
    49501 tcgcttcggt ttcaatggct tcagccgcca taaaggtgtg tgctacgcca gtcgggcaag
    49561 cagtcaccgc aactacgcgt ttcggaccgc tggcggcaac cggtgctgtc gcagtgaccg
    49621 gcgcagtgta aggtttcgca tggcctttgg cttcactcag gaacagctca gggtgcgcaa
    49681 ctgcccggga aatatcaccc agccagacat ttttaccgtt cagcgcgctg tcgttcggga
    49741 tggaatcacc gagaacaatc gccatttcag cgtcgttcgg attgtcgatg atttccagtt
    49801 ttgcttttcg cgccgccgcg cccagcaggg tcttcgccat ataggcgcgt gcctgaccga
    49861 gattagcgtc aataatcagc agcgttttca ttatgcctct cctgctgtca gttaaaaggt
    49921 tgtaagtcga cgcgcgccat cattgcggcc aactgcggac gatcggtaat acccacattg
    49981 ctttgactta ccgccagggc tgcaacagct gtcgccagac gcagtgtgtg ttcactggat
    50041 tcacgcatca gcaagccata aatcaggcca ccaaccatag aatcccctgc gccaacggtg
    50101 cttacgacat cgactgacgg tggtttggcg atccattcgc cggaggcatt aacccaaagc
    50161 gcgccttcgg cacccagtga aataacaaca tgcgcgatgc cttgttcacg tagcgcatgt
    50221 gcagcttcaa tcacatcttt catttcaggc agtttacggc ctgcccagat ttccagctcg
    50281 cggcggttag gtttcaccag ccacggtgcc gctttcaaac ctgctactaa cgcttcacgg
    50341 ctactatcaa agataatgca aggacactga ctacgcaggc gagtcatcca gtcggtgaac
    50401 gcttccgggc tgacgcctga cggtaagctt ccgctgacac agaccatatc gaactgaccg
    50461 agccagctca gagaatcagt cacaaagcgt tcccagtcgg cgggggtgac ttcaaaaccc
    50521 gagaagttga agtcggtcac ttcaccgtct ttttccgtca gcttaacgtt aattcgggtg
    50581 cgcccctgta caacctggaa acggttggca atgcccagct cgctgaacag ttgctgaaaa
    50641 ccatcctgat tgtctttacc gaggaagccg ccaacggtga catcaattcc caggtctttt
    50701 aatactttgg ccacgttgat gcctttaccc gccgcatgca gaccggtggt tttcaccagg
    50761 ttcacttcgc cgcgttcaat ttccgggcag aaaccaacaa ggtcataagc cggattaagg
    50821 gtgatagtag caacacgtct gctcattatg cgccctcccc aagaccagca gcgatagcgt
    50881 cgccaatggc tttcagcgcc tgttcagcat ctccaccctg ggcggtaaag cgtaggcgat
    50941 gacctttctt aacgccaagt gccacaactt tcatcagact acgtccgttt gccggtttgc
    51001 cggtgccatc aaggtttgtc acggtaatat cactgttaaa ttgtttaatg gtattgacca
    51061 gcatggtacc tggacgagca tgcaggccgt gttcattgcg caccacaaac tccgcgctta
    51121 acacgtcgtc ggtcggcgca tcatcgctgg tcagcagtgc cagcaacgtt gccgcatccg
    51181 ctttcagcaa gcggtcagct ttattgtcga gcaacaaatc agcgagacgc ttaagaaccg
    51241 cgatgggctg atcgtcattc atcgctacac tcaccagcat ggctgccgtt tcgccgtcca
    51301 catcaaaagc atttgccgca cggcttaccg caatcgcgct acgcagattg ccttcggcgc
    51361 tatcgctcag ccagatgccc tgtccgaggt tcagcggttg ttcattgatg gctttggtga
    51421 cgaaagtggc gtcaactgcc cccgcctctt tcagacgcgc agcgttcagc gcctgaagag
    51481 tcagcagatc gctggcgacg atatccagtg tcagcatttc gttgtcgagc ttcagctgct
    51541 cactctgctt ttcgcccatc agcaatgcgc gaagttcttc tgctgttgtt gctgacttca
    51601 gttgttcagc aacggaatca tcactcagta cgtgggtcag ctggcgtagc aggcccagat
    51661 gttcatccga gctggcagca ataccgatcg ccacgtacgc tacctgaccg tcaccccagg
    51721 tgacgccttc cgggaactga aatacctgaa cgccggtttt cagcacctga tcgcgggtgt
    51781 cggtggtgcc gtgtggaata gcaataccat tgccgaggaa cgttgaggtt tgctgttcgc
    51841 gcgccagcat gccattgacg tagccttctg ctacgttacc ggcctgcacc agcgccgcag
    51901 caacctggcg aatcgcctct tctttgtctc cggccttttc gcccggatgg atgtcctgta
    51961 cggataactg gaacatagtt ctcctctctt gctgaattga aacgattcag cctctatgag
    52021 aaaaaaagcg ccaacctggc ttagggttaa agacaagatc gcgctgaaac gtttcaagaa
    52081 agcataatac ttctgattca gcacgcaagg aaagccgtaa agttatgagc aaaagtttga
    52141 tgtgctgcac aattttttgt catttttcct aattgctgat gggaaaatcc gttatcagca
    52201 atttcatttc agcagcgtca gcagccaatc agcaacagtg aaatggcatt ttgattattc
    52261 gtggcgcttt tgatttcgct gtctgtttat tttctgacaa gcagcgtaaa ctccgcgtct
    52321 tcctcttcca gtgatcgacc agcatgcata actcccccgc agtctccagc gcgaaatcgt
    52381 ttgacctgac ctcgacggcg tttttaatcg ttgcctttct caccggtatt gcgggcgctc
    52441 tgcaaacccc gacactcagt atttttctta ctgatgaagt acatgcccgt ccggcgatgg
    52501 tgggattctt ctttaccggc agcgctgtca ttgggattct ggtaagtcag tttctcgccg
    52561 ggcgctctga taagcgcggc gatcgcaaat cgctgattgt cttttgctgc ctgttaggcg
    52621 tgctggcctg cacccttttt gcctggaatc gcaactactt tgttttgcta ttcgttggcg
    52681 tctttcttag cagctttggc tcgaccgcta acccgcaaat gtttgccctt gcccgtgaac
    52741 atgccgacaa aaccggacgt gaagcggtga tgttcagctc ttttttacgc gctcaggttt
    52801 cactggcatg ggtcattggc ccaccgctgg cttatgcctt agcgatgggt ttcagcttta
    52861 cggtaatgta tctgagcgca gcggtagcat ttattgtttg cggcgtgatg gtgtggctgt
    52921 ttttaccgtc gatgcaaaaa gagcttccgc tggcgaccgg cacggttgaa gcgccgcgcc
    52981 gtaaccgtcg cgatacgctg ctgctgtttg tcatttgtac attgatgtgg ggctcgaaca
    53041 gcctgtacat catcaacatg ccgctattta ttatcaacga actgcatctt cccgagaaac
    53101 tggccggtgt gatgatgggg accgccgccg ggctggaaat cccgacgatg ttgattgccg
    53161 gatatttcgc caaacgtctg ggtaagcgtt tcttaatgcg cgttgctgcc gtgggtggcg
    53221 tctgttttta cgcaggaatg ctgatggcgc attcacctgt cattctgttg ggcttgcagc
    53281 tgctaaatgc tatttttatt ggcattctgg gcggcatcgg gatgctctat tttcaggatc
    53341 tgatgcccgg tcaggcgggt tcagccacca cgctctatac caacacgtcg cgcgtgggct
    53401 ggatcatcgc aggatcagtg gcgggcatcg tcgccgagat ctggaattat cacgctgtgt
    53461 tctggttttc gatggtgatg attatcgcca ctctgttttg cttactgcgg attaaagatg
    53521 tttaaggcgc ggtcagcatt tccagatcga taagccaggt catggcctgc tgacgcgagt
    53581 taccgcacat ttcagcgctg gcttgtaagc ctgcacagac tttcggtcgc agtggcgagg
    53641 tgaaaatttt acagcgttgc tgttcatcaa gctgaatgca gggcgtattg gcgggcttgc
    53701 catctggcat accgggaata gggctggaaa ttgaaggggc agtgcaacac gccccgcaac
    53761 ccggacggca ttccatacgt tgttctctta aggttaacca ccggattgat gagcgcgcac
    53821 agtaccacct tttttgcacc agcaaaagtg cgaataccac ttgcccgaaa ggcccgtcgc
    53881 gagtactttg tcgcgatatt tttgacattt tcgactacag gaatttttcg atgccaagag
    53941 cgaacgaaat taaaaaaggt atggtactga attacaacgg caaactgctg ctggttaagg
    54001 atattgatat tcagtcgccc actgcccgcg gtgccgctac gctgtacaaa atgcgttttt
    54061 ctgatgtccg taccgggctg aaagtagaag agcgtttcaa aggtgatgat atcgttgaca
    54121 ccgtgacgct gacccgccgt tacgttgact tctcctatgt cgatggcaac gaatatgtct
    54181 ttatagataa agaagactat accccgtata ccttcaccaa agatcagatt gaagaagagt
    54241 tgctgtttat gccagagggc ggcatgccgg acatgcaggt gctgacctgg gacggtcaac
    54301 tgctggcgct tgagctgccg cagaccgttg atctggaaat cgttgaaacc gcaccaggca
    54361 tcaaaggggc atctgccagc gcccgtaaca aaccggcgac attgagcact ggtctggtga
    54421 ttcaggtacc agaatactta agcccgggcg aaaaaattcg tatccatatc gaagaacgcc
    54481 gttatatggg gcgtgctgac taacttcagc cgcatgcaga aaagggatag ctcaggctgt
    54541 ccctttttta atttattata ccaatcttct attttgcgct tcctgaacac cttacgccct
    54601 caattttcac tcgttgattg atcgccctca cacttcatcg cattaacaat ccagaccaat
    54661 ttcaattgct gtcatataac tttacactgt cattgttaat ttatcgttac taagacgtga
    54721 ctcctatgaa gacaattgcc tccgttacgc tcccgcatca tgtacacgct ccacgctacg
    54781 atcgccagca gttgcaatca cgtatcgttc attttggctt tggcgccttt caccgcgctc
    54841 atcaggcgtt actgaccgat cgtgtgctga atgcccaggg cggcgactgg gggatctgtg
    54901 aaatcagctt gttcagcggt gatcaactga tgagccagct ccgcgcacag aaccatttat
    54961 ataccgtgct ggagaaaggt gcggacggca atcaggtgat aattgtcggt gccgttcacg
    55021 aatgccttaa tgcaaaactg gattccttag cggcaattat tgagaaattt tgcgagccac
    55081 aggtggcaat tgtttccctg acgattaccg aaaaaggcta ttgtattgac ccggccaccg
    55141 gtgcactcga caccagtaat ccgcggatta ttcacgatct acaaacccct gaagaacctc
    55201 actccgcacc gggtattctc gtcgaagcac tgaaacgccg ccgtgagcgc ggccttacac
    55261 cgtttaccgt gctctcctgc gacaatattc ccgacaatgg ccatgtggtg aaaaacgcgg
    55321 tgctgggaat ggcagaaaaa cgttcgccag aactcgccgg gtggataaaa gaacacgtca
    55381 gttttccggg aaccatggtc gaccgcattg ttccggctgc aaccgacgaa tcactggcag
    55441 aaatcagcca gcatctgggg gtaaatgatc cctgcgcgat tagctgcgaa ccgtttatcc
    55501 agtgggtggt ggaagataac ttcgtcgctg ggcgtcctgc ctgggaagtc gcaggtgtac
    55561 aaatggtgaa tgatgtcctg ccatgggaag agatgaaact gcgtatgctt aatggcagcc
    55621 actcttttct cgcttatctg ggttaccttt caggattcgc ccatatcagt gattgcatgc
    55681 aggatcgcgc atttcgccat gccgccagaa cattaatgct gaatgagcaa gcgccgacac
    55741 tacaaattaa agatgtcgat ttaacacaat atgcggacaa gttaattgca cgttttgcta
    55801 atccggcgct gaaacataag acctggcaaa tcgcaatgga tggtagccag aaattaccgc
    55861 aacgtatgct ggcaggtatt cgcatacatc tggggcgcga aacggactgg tcgttgctgg
    55921 cattaggcgt tgctggctgg atgcgttacg tcagcggcgt tgatgatgcc ggaaatgcca
    55981 ttgatgttcg cgatccgctt agcgataaaa ttcgcgaact tgttgcgggc agcagcagtg
    56041 aacaacgcgt aaccgccctg ctttccctgc gtgaagtttt cggtgatgat ctgccagata
    56101 acccgcattt tgtgcaggcc atcgaacaag cctggcaaca aatcgtacaa ttcggcgcac
    56161 atcaggcgct attaaacacc ctcaaaattt aacgatttct gcggttaaag cggatgaagc
    56221 tcaccttcgt ccgctctccc cttctctttt ctgccttttt tagccaggat taacgctcag
    56281 ttaacttacc agagtattgc ggagccgttg tgaccaggac caacctcatc accggttttc
    56341 tcggcagcgg gaaaaccacg tcgattcttc atctgttagc ccataaagat cccaacgaaa
    56401 aatgggcggt actggttaat gaatttgggg aagtcggaat tgatggtgct ttgctcgccg
    56461 atagcggcgc attgctgaaa gagatccccg gcggctgcat gtgctgcgtt aatggtttac
    56521 ccatgcaggt agggttgaat accttactgc gtcagggaaa accagaccgc ttgttgatag
    56581 agccgaccgg gctgggccat ccgaaacaga tcctcgatct gttaaccgca ccagtctatg
    56641 aaccgtggat agatctgcgc gccaccttgt gcattctcga tccacgcctg ctgctggacg
    56701 aaaaaagcgc cagcaatgaa aacttccgtg accagctggc tgccgcagac atcattgtcg
    56761 ccaataaatc cgaccgtgcg acgcccgaaa gtgagcaagc gctacagcgt tggtggcagc
    56821 aaaatggtgg cgatcgacaa ttaattcaca gtgagcatgg gaaagttgac ggtcatcttc
    56881 tggatttgcc gcgtcgcaat ttagccgagt tgcccgccag cgccgcgcat tctcatcagc
    56941 atgtcgtgaa aaaagggtta gcagcgttaa gcctgccaga gcatcaacgc tggcgtcgca
    57001 gtctgaacag cgggcaagga tatcaggcct gcggctggat attcgacgct gatacggtat
    57061 tcgacaccat tggcattctg gaatgggcgc gacttgcacc ggtggaacgc gtcaaaggcg
    57121 tgctgcgtat tcccgaaggg ctggtacgaa tcaaccgtca gggcgatgac ctgcacattg
    57181 aaacgcaaaa cgttgcgcca ccggacagcc gtattgagct gatttccagc agcgaagctg
    57241 actggaatgc tttgcagagc gcgctgttga agcttcgttt agcgactacc gcgtaaggtt
    57301 gcctgcgttt ttcagtaaga taattagaga a
//
LOCUS       DAFNBJ010000010        54339 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000010 DAFNBJ010000000
VERSION     DAFNBJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.010"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..2299)
                     /gene="pbpC"
                     /locus_tag="L2X49_000621"
     CDS             complement(<1..2299)
                     /gene="pbpC"
                     /locus_tag="L2X49_000621"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HBN4795762.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDHWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVK"
     gene            complement(2300..7261)
                     /gene="yfhM"
                     /locus_tag="L2X49_000622"
     CDS             complement(2300..7261)
                     /gene="yfhM"
                     /locus_tag="L2X49_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HBN4795763.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGLTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAGFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPDETVADQQKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            7468..8313
                     /gene="sseA"
                     /locus_tag="L2X49_000623"
     CDS             7468..8313
                     /gene="sseA"
                     /locus_tag="L2X49_000623"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBN4795764.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(8806..9582)
                     /gene="sseB"
                     /locus_tag="L2X49_000624"
     CDS             complement(8806..9582)
                     /gene="sseB"
                     /locus_tag="L2X49_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBN4795765.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPSDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(9724..11007)
                     /gene="pepB"
                     /locus_tag="L2X49_000625"
     CDS             complement(9724..11007)
                     /gene="pepB"
                     /locus_tag="L2X49_000625"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBN4795766.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(11185..11385)
                     /gene="iscX"
                     /locus_tag="L2X49_000626"
     CDS             complement(11185..11385)
                     /gene="iscX"
                     /locus_tag="L2X49_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBN4795767.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(11397..11732)
                     /gene="fdx"
                     /locus_tag="L2X49_000627"
     CDS             complement(11397..11732)
                     /gene="fdx"
                     /locus_tag="L2X49_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBN4795768.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(11734..13584)
                     /gene="hscA"
                     /locus_tag="L2X49_000628"
     CDS             complement(11734..13584)
                     /gene="hscA"
                     /locus_tag="L2X49_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBN4795769.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(13601..14116)
                     /gene="hscB"
                     /locus_tag="L2X49_000629"
     CDS             complement(13601..14116)
                     /gene="hscB"
                     /locus_tag="L2X49_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBN4795770.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(14212..14535)
                     /gene="iscA"
                     /locus_tag="L2X49_000630"
     CDS             complement(14212..14535)
                     /gene="iscA"
                     /locus_tag="L2X49_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBN4795771.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(14552..14938)
                     /gene="iscU"
                     /locus_tag="L2X49_000631"
     CDS             complement(14552..14938)
                     /gene="iscU"
                     /locus_tag="L2X49_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBN4795772.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(14966..16180)
                     /gene="iscS"
                     /locus_tag="L2X49_000632"
     CDS             complement(14966..16180)
                     /gene="iscS"
                     /locus_tag="L2X49_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBN4795773.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(16292..16780)
                     /gene="iscR"
                     /locus_tag="L2X49_000633"
     CDS             complement(16292..16780)
                     /gene="iscR"
                     /locus_tag="L2X49_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBN4795774.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(17081..17818)
                     /gene="trmJ"
                     /locus_tag="L2X49_000634"
     CDS             complement(17081..17818)
                     /gene="trmJ"
                     /locus_tag="L2X49_000634"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBN4795775.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            17937..18740
                     /gene="suhB"
                     /locus_tag="L2X49_000635"
     CDS             17937..18740
                     /gene="suhB"
                     /locus_tag="L2X49_000635"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBN4795776.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            18885..19739
                     /locus_tag="L2X49_000636"
     CDS             18885..19739
                     /locus_tag="L2X49_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN4795777.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            19930..21210
                     /gene="csiE"
                     /locus_tag="L2X49_000637"
     CDS             19930..21210
                     /gene="csiE"
                     /locus_tag="L2X49_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBN4795778.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(21202..22341)
                     /locus_tag="L2X49_000638"
     CDS             complement(21202..22341)
                     /locus_tag="L2X49_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBN4795779.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(22501..23391)
                     /gene="hcaR"
                     /locus_tag="L2X49_000639"
     CDS             complement(22501..23391)
                     /gene="hcaR"
                     /locus_tag="L2X49_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBN4795780.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            23527..24888
                     /locus_tag="L2X49_000640"
     CDS             23527..24888
                     /locus_tag="L2X49_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBN4795781.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            24885..25403
                     /locus_tag="L2X49_000641"
     CDS             24885..25403
                     /locus_tag="L2X49_000641"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBN4795782.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            25403..25723
                     /locus_tag="L2X49_000642"
     CDS             25403..25723
                     /locus_tag="L2X49_000642"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBN4795783.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            25720..26532
                     /gene="hcaB"
                     /locus_tag="L2X49_000643"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             25720..26532
                     /gene="hcaB"
                     /locus_tag="L2X49_000643"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBN4795784.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            26542..27744
                     /locus_tag="L2X49_000644"
     CDS             26542..27744
                     /locus_tag="L2X49_000644"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBN4795785.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            27841..28263
                     /locus_tag="L2X49_000645"
     CDS             27841..28263
                     /locus_tag="L2X49_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBN4795786.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(28311..29183)
                     /locus_tag="L2X49_000646"
     CDS             complement(28311..29183)
                     /locus_tag="L2X49_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBN4795787.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(29195..30289)
                     /locus_tag="L2X49_000647"
     CDS             complement(29195..30289)
                     /locus_tag="L2X49_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBN4795788.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(30322..31320)
                     /locus_tag="L2X49_000648"
     CDS             complement(30322..31320)
                     /locus_tag="L2X49_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4795789.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(31345..32856)
                     /locus_tag="L2X49_000649"
     CDS             complement(31345..32856)
                     /locus_tag="L2X49_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBN4795790.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRVAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(32879..33862)
                     /locus_tag="L2X49_000650"
     CDS             complement(32879..33862)
                     /locus_tag="L2X49_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBN4795791.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSHVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVLAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(33959..37240)
                     /locus_tag="L2X49_000651"
     CDS             complement(33959..37240)
                     /locus_tag="L2X49_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBN4795792.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPERAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            37358..38551
                     /locus_tag="L2X49_000652"
     CDS             37358..38551
                     /locus_tag="L2X49_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBN4795793.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAQEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(38615..39868)
                     /gene="glyA"
                     /locus_tag="L2X49_000653"
     CDS             complement(38615..39868)
                     /gene="glyA"
                     /locus_tag="L2X49_000653"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBN4795794.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            40197..41387
                     /gene="hmpA"
                     /locus_tag="L2X49_000654"
     CDS             40197..41387
                     /gene="hmpA"
                     /locus_tag="L2X49_000654"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBN4795795.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(41432..41770)
                     /gene="glnB"
                     /locus_tag="L2X49_000655"
     CDS             complement(41432..41770)
                     /gene="glnB"
                     /locus_tag="L2X49_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBN4795796.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(41831..43165)
                     /gene="glrR"
                     /locus_tag="L2X49_000656"
     CDS             complement(41831..43165)
                     /gene="glrR"
                     /locus_tag="L2X49_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBN4795797.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(43155..43868)
                     /gene="qseG"
                     /locus_tag="L2X49_000657"
     CDS             complement(43155..43868)
                     /gene="qseG"
                     /locus_tag="L2X49_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBN4795798.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(44033..45460)
                     /gene="qseE"
                     /locus_tag="L2X49_000658"
     CDS             complement(44033..45460)
                     /gene="qseE"
                     /locus_tag="L2X49_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBN4795799.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GILPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLKPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(46018..49905)
                     /gene="purL"
                     /locus_tag="L2X49_000659"
                     /gene_synonym="purI"
     CDS             complement(46018..49905)
                     /gene="purL"
                     /locus_tag="L2X49_000659"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBN4795800.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAIAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            50163..51719
                     /gene="mltF"
                     /locus_tag="L2X49_000660"
     CDS             50163..51719
                     /gene="mltF"
                     /locus_tag="L2X49_000660"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBN4795801.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(51716..52252)
                     /gene="tadA"
                     /locus_tag="L2X49_000661"
     CDS             complement(51716..52252)
                     /gene="tadA"
                     /locus_tag="L2X49_000661"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBN4795802.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(52277..52912)
                     /gene="yfhb"
                     /locus_tag="L2X49_000662"
     CDS             complement(52277..52912)
                     /gene="yfhb"
                     /locus_tag="L2X49_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBN4795803.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            53121..53969
                     /locus_tag="L2X49_000663"
     CDS             53121..53969
                     /locus_tag="L2X49_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBN4795804.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            54025..54285
                     /gene="yfhL"
                     /locus_tag="L2X49_000664"
     CDS             54025..54285
                     /gene="yfhL"
                     /locus_tag="L2X49_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBN4795805.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
BASE COUNT        12781 a        15014 c        13789 g        12755 t
ORIGIN      
        1 atttcactgt cgcgatttgc cccacatcat ccatcaccag caattgataa tcacctttat
       61 ccgtcaaatg cagggtcacg ttgcgcccgc gttcagttaa cggttcgcca ttcagaaacc
      121 accagcgttc acctgcccct ccacttgatt gcaacggcaa agtcgcttcc gctgcgcccg
      181 gtaaacgttt aataatcgcg ccatcgcgaa cgcctgtcag ctgcagaggg agcttggcat
      241 cgtgaccgta tggcgggcaa attgtcgagg ccggtggtaa gcgcacagcg cgacgttctg
      301 atgcgggcag ccagggttcc agcggtagcg gccagacatt tatcatttct tgtcgcgctt
      361 gcgggcaatc agcggcaaca cgtttgccat tttcatccag ccagatgggg aaacgaatgc
      421 cattaatgcc ttcctgctcc ggcagtaata gagttggcgg ctgactcccg tccagcagcc
      481 aggttgccag gcggcggcga cagttaccgt caccttccgg caaagactgt ccgcccggcc
      541 agcagataac gccacgagtg actgagttcg ggcgcgggtc ttccggcaga ttcgcactgc
      601 gcgacagtaa gatattattg acctgattca acaatggtac ggcactggca aagccaaact
      661 gaccaacaac gggcgtgccg tccggtctgc cagtccagat cccaatgaca tagcgagcgt
      721 taaccccaat cgcccaggcg tcacgatagc catagctggt gcccgttttc catgccagtg
      781 gggcgacgcg cggcaaggca ctatccggca agggtttcgc ttcatcagcc ataatccggc
      841 gaatgatcca cgccgccccc gacgacatta aaggccgttc aagcagcgga tcgtcaggct
      901 gtaagcgcaa tttgcctgcc ttgccgtggc gagcaaacgc ggtatacgct gccgccatat
      961 cttccagttt tgcaccagcg ccgccgagaa tgagtgaaag attcggcgca gcaccgttgg
     1021 gcaaatataa cggcaatcca acattgcgta actttgccgc aaaccgtttc ggtccatagg
     1081 cttccagcac ctgcacagca ggtaagttca gcgagcgcac cagcgcctcg ctcatgctga
     1141 tcgggccatg aaaaccgcta tcaaagttac ctggtcgata atcaccggtg cgccggggga
     1201 cgtcttgcag cagtgatgcc gggtggatca agccttcatc cagcgccaga ccataaacaa
     1261 acggtttgag caccgatcct ggcgatcgga tcgcattgac catatcaaca tgaccaaagc
     1321 gtgaatcatc gttgagatca accgatccca cccagccgcg aacacgcata tcggtatgat
     1381 caaccacgat catcgccagt gagctgcgcg gtggcaaccg ccctttccag ttttgcgcca
     1441 gttcttccag acgtcgttga agaccggcat ccagcgtagt aacgattttg tcgcttttgc
     1501 ttttaccgag catcatgcgc gaaaacagcg gtgccagttg cggcatttgt cggggtgcca
     1561 gccagatggg ttcttccctt gactctttta cctgctcacg ggaccacaca ccttgcacgg
     1621 ccatccgttc gagcacttta ttacgcgcgg cttcggcacg ctccggccaa cgatccgggc
     1681 gaagacggct gggcgcttgc ggcaaaaccg ccagcattgc cgcctcggaa tagcttaaat
     1741 tcgcaggcga ctttccgaga taagcccaac ttgccgcacc gatcccctgc aacgtaccgc
     1801 caaacggagc gcggttaaga tacaaggtca gaatttcacg cttagacaga tgccattcca
     1861 gttgcaacgc gcgccagagc tggcgaattt tgccgccaaa tgttttgggg tgaggatcaa
     1921 gcagacgagc aacctgcata gtgagcgtgc tgccaccgga aataacccgt cccgaagtga
     1981 gatcttgcca tgctgcgcgc gccaccgaga atggattcac ccccggatgc ttccagaacc
     2041 agtgatcttc ataattgatc agcgcttcaa ggtaacgtgg agaaacatct tcgattgtta
     2101 ccggataacg ccagatgccg tcagcatcgg cgaagcgcca tagcggcgta ccatcctgcg
     2161 ccacgaccac tcgtgcggga ttgacttcat gcagcggtag aggccagagt ttatctgctc
     2221 cccacgctgc aagaaaaagg agaaatggcg cagctgccag cgttatccag cagccgcgtt
     2281 tggttaacaa gcgaggcatt tacggtcgga caatcagcag atcttcagcc gcgccggtcg
     2341 cccgccattg gggaacatac attgattcca ccatcggttg cggtacctga tacgttcccg
     2401 gcgtcaccgc ccgcgccaga tacaccagcg ttaccggttg gtattcatca acggcaaccg
     2461 ccgccacaaa gcgatcgtca cggaactcaa tgtgcttaat actcgcctgc tgcatctggt
     2521 tcagtaagtt ctgcacttcg ccaccacttt gctccaggct ggcgctaccg ttcgccagat
     2581 tctgattttc cagttccaga cccgcaggca gcagatccac gactaacgca tccggcacgc
     2641 tgttactggc ttttacctgc aaccagacca gcaccagatc gccgctacgt aacgagtcca
     2701 gcgatttgct cttaccatca gtaccaagaa tatgacgctc gatttgcagc acattgttcg
     2761 ccggtaaagg tgcggattgc ggataaccgc tggcatccat acgcaaccat aacggctgat
     2821 cgccactgtt tctcacctgc aaggtggcaa gttgatcgct attcagattg ctgtttagcg
     2881 ttttctcgcc tgtcagcggc tcagctgaga aagaggtttg cgcctgccat ttacccggta
     2941 aatcctgaat cgtacgggca gctaggaaca acgcgttact ttcctgcgtc gatagccagc
     3001 gttcaccaaa cgcctgctgc gaaagtgtgt tcagcaaagt gtactgctca tcgggtagca
     3061 gtttattttc ttccagcaag gagagcatta acgcgttgtc gcgcagtgga ctaccgtaat
     3121 cacccagcca tatccgctca tcactatttc tcggcgtttt cagcgccagc acaatcgctt
     3181 cttcaccacg cgttgcatca cccatggttt tcagcgcaac gccaagttgc agcagcggta
     3241 aaccagaagc ggcatctgca cgatgctccc agatttcacg cagcgcaccc agcggagcct
     3301 tttgttgacg ggccaacacc agcgcagcgt aagactgtac ggcgaattta ctggctttga
     3361 gattatccgc gtacgggatc gacatcatgc ccggatcttg taaatagcgc agcagacgct
     3421 cattaccccg gttaatggcg tctgtcggca cgctgtaacc ctgttcgcct gcgcggacca
     3481 ggaaatccat cacgtaagcc gtcagccagt actcttcgtc accgttttta tcccacagcg
     3541 caaagccgcc gttatcacgt tgcatttgca gcaaacggga aatgccgata tcgaccgatg
     3601 cacggcgttt ctcatcactg tcgcctttga tgcccaacgc ctgcagttgg gctgcgttgg
     3661 tataaagtga cgggaacagg ccgctggcgg tttgctcaag acagccgtac ggatacgctt
     3721 ttaactcttt gatataacgt gcaatgttca gcggtggttt gccgctcaac aacaattgcc
     3781 cttccagcgt aacaggcgag aagttttgca atccatccgc cggaatcgcc catgtctcac
     3841 caggctgtaa cgccgtaccg taattaaccg tttgtgccgg gaacgccgga cggacgccga
     3901 ttttccactg cttctgctga tcggcaacgg tttcatccgg taacgctaac ccgctaatgg
     3961 tggcctgaat ttctccatcg ccataacctg gcaatgctcg caccgggata aacagcgtag
     4021 tacgcacacc tggcgctaat tcaacggctg cgggtgaatc gctgaccagt tcaagcaaac
     4081 cactggcggt cagggcaacg ttcagttttt gcggtttatc ggtaagatta gtgatatcca
     4141 gcgtcagacg cgaggtatcg ccactcgcca taaagcgcgg catgttcagt tcagcaatca
     4201 ccggtgcggc aactatcact ttgctttcgt tgctaccgaa gtcatctgcc gtccaggctt
     4261 gcgccatgac gcgtaattca ccgttaaaat cgccaatcgg cagtgtaacc gagccttcgc
     4321 cctgttcgtt gagcgttacc ggcagcgcct gctgcgcgac aatattgacg tgattgaccg
     4381 gcggtttacc accacgtttc agctcatcac catcgccacc gaaacgcaga gctgccagac
     4441 gcccctgacc ttcaataacc tgaccgtaaa tatcgtaaat gtctgcgcca tagcgtttct
     4501 gaccaaagaa cgcctgccac ggatctggcg tgacgtagtc agtaatattc agcacaccac
     4561 tatcaacggc agacaccagc acattcacct gtttaggctt ctcgccattt ttagtgctgg
     4621 ctttaatttt cacggttaat ggttgattcg gccgcatttt tgctggtgtt tccagcgcca
     4681 gatcgaggcg acggttttca tcgccaagcg gcagatgcaa caccccaact gcgcgttttg
     4741 gcgtcgcgga gcgagattta tcgccaggac gtactaccag cgtacttaaa tacagatcat
     4801 gacgattcca ggttttatcg accggaatcg tcagatccag cccttgagcc ggaacatcaa
     4861 tctcttgcca ccacaacggc ccttcactgg actcgaccat cgcataacct ttacccgccg
     4921 ttggcgcggc aatatgcaac ttaatggtat cgccagggcg ataactggct ttatccagtt
     4981 tcagcgtgac acggtcgggt cgcacggcac cgctcccgtc gctgttgtcc tgccagctat
     5041 agccagccca gaaacgcacg ctactgaccg cttcattcgg cgctttgact tccagacgat
     5101 aagcgcccca ctctaccggg aaactgacct taccggtttc atccgcctga agatccagag
     5161 tttgttcatt ttcgatcaga tctttttgat caaactgtga ctgccagcct tcatcttctg
     5221 accagttcca gtagtaatcg cggcgttcgc gaatcaggcg cacctgcaag cccgacacgg
     5281 cttttttcac gccttgcgca tcgctataaa cgatgtcaaa accggcgtta ctgccttcat
     5341 caacaatggg ctgtttcacc gtgctgtcag tgcgataatc gtaaaccgat ttcgaggcga
     5401 actgcggacg gatccccggc aatgcatcgg caggccagat agcctgctca gcgcggcgcg
     5461 tcaccgggcg accgcccgat tccagcaaac taccctggaa aataacctgt aatggggaat
     5521 gcgtttcctt ccactggctt tctgtagaaa cttcgccgcg ccctttatca tccagcgtca
     5581 actgaacttc atccagcgtg cgggaaagat tttcggcagc tatatcgccg aattcaaaac
     5641 caggtaaggc tgacacagct tcacgcagtg ggcgcaggaa aagttgccct tgcaaagtat
     5701 taccattagc aggtgcacca tacaggtagt accccaccac ggagaatttc acttcatctt
     5761 tcggcgttag cggggttttc tcaccggtca gattcagcgc catgcgctct ggcataaaat
     5821 cttcgacgtg gaaatcccac atccgatact ggttatcgcc cgtgttagcg cgaatatgcc
     5881 acataccggt tgccgcattg ctatcgagtg gccaggtaaa gtggtagagg ccattctccg
     5941 gctgactaac gacgctcctg agtacctgcc catcgggttt aatcacgtct aacttgatgg
     6001 gttgattggg caacgcttta ccgtctgcat cacgcagcaa accattgagg attaccgttt
     6061 cacctgggcg ataaagatcg cgtgggccaa acatgaaaaa ctgtttgcta tagcctggcg
     6121 cgccagcaat gttaaattct gctaagtcca gcgccggaag ttttaaatcg agtagcgttg
     6181 tctgaccgtc tttacgcgcc aacagtaatg ccgcgttttt atcattttcc agctgcacat
     6241 gcccctgagc gtcactggtt gcctgagtca gagtcagccc tttctcattt aataaagaga
     6301 cttcaattcc ttgctgggcc gcgccgtttt ccagactttg ggtaaagata tccagacgat
     6361 tgtgataacg gtgagctgaa acgccgatat cacttaacgt aaacagcgtc gcgggattac
     6421 tgtaatcgta acgtccagcc tgattcatca cagccagata cacgcccgcc tgctgaagcg
     6481 gtttgatatc gcccagcggc agcaataatt tttcacgggt gttacgcgca ggattgagat
     6541 caaaccgtcc ggtgtagacc agatccgcca tctgcagcag tttgtctgac tgccagttcg
     6601 ccagcgaatt gcggtattcc cattggctaa tgaatgctgg cagagattct ggcttaacgc
     6661 ggaagaagtt aacatcgaca ttattaacgt tgagcgccat taccggcagc ccttcaacga
     6721 ctttgccagg cagcagcgaa ccacggctgg caaaaccgac gctgggttgg atgtcgcggg
     6781 tagttatagt tttttcgtaa tctttactga aggttgcgtt gttgagtgct ttgacctcct
     6841 tgccaatagt aacgatcaaa tcacgtttcg gttcgaggtg gcgtaaacgt aactctttaa
     6901 gattatctga cagctcccag gcaccatcca ctttgccgct ttttttatcg acgacatgaa
     6961 taacgcgtga gaaatcctga tccgggtcga gagggatgga gaacgtcagc accagcgtgg
     7021 ctgcaccatc aagttggact tcagagagat ccagcaatgt cagcacctta ccggcactct
     7081 gttgggccag tttttgtctc tccggcacgg agagttttgc tgaactcgcg ttttctgaag
     7141 aggccgcttt agtaacttca gaaggcgcat cttttttcac cgctgttggc gcgttatcgt
     7201 tgttgtcgca ccctgccagc gccagcatta gcatgcaggc ggctacgcgt aactttttca
     7261 tttttcatcc ctgccacaat ggcccgttag caacgtcgaa taattattat gcgtgagaat
     7321 ttacgttatg taattcagta tcacgcccat acaatccgaa ttttagtgag aattgtggct
     7381 cagacagcca aatcagcctc ttttgcttgt cgcttttcac taaacatccg acaatttagc
     7441 ctaaccccgg caaaaatgga gatgcctatg tccacgacat ggtttgtagg agccgactgg
     7501 ctcgccgaac atattgatga cccggaaatt cagattatcg atgcccgcat ggcgtcgcct
     7561 ggacaggagg atcgtaacgt tgctcaggag tatctgaatg gacatattcc cggcgcagtg
     7621 ttttttgata tcgaagcgct ttctgatcac acttccccgc ttccgcacat gctgccgcgc
     7681 ccggaaacgt tcgccgtggc gatgcgtgaa ttaggcgtta accaggataa gcacctgatt
     7741 gtctatgacg aaggtaatct tttctcagcc ccacgagcat ggtggatgct gcgcaccttt
     7801 ggtgtagaga aagtgtcgat tctggggggt ggacttgcag gctggcagcg cgatgatctg
     7861 ctgttagaag aaggtgcagt agagctgccg gaaggagagt ttaacgccgc gtttaatcct
     7921 gaagccgtgg tgaaagtaac cgatgtatta ttggcaagcc atgaaaatac ggcgcaaatt
     7981 attgatgccc gcccggctgc acgttttaac gcagaagttg atgaacctcg cccaggttta
     8041 cgtcgcggac atattcccgg agcactgaat gttccgtgga cggaactggt gcgcgaaggc
     8101 gaactaaaaa cgaccgatga actggatgcg atattttttg gtcgcggcgt cagctacgac
     8161 aaaccaatta tcgtcagctg cggctctggt gtaacggcag ccgtggtttt gttagcactc
     8221 gcgacgctgg atgtgccaaa cgtgaaactg tacgacggcg catggagtga atggggcgcg
     8281 cgggcagatt taccggttga gccagtgaaa taagtatttt acaggcaata aaaaaccgcc
     8341 gaatttggcg gttttttatt gctggtctgg ttcgcggcct ttccagcagg ttgtattacc
     8401 gtagtaatgc aagcgcgtct cagcggagac aatactcgcc agtaactctc tttttgtcaa
     8461 gcaaaagaga gttattattg ttctgttagt gtattatcca ctgcggccct ttccgccgtc
     8521 tcgcaagcgg gcgctggctt aaggaaagga tgttccgcga ccgttagagc aagcgcgtct
     8581 cagcgcttgc tatcgcggca atatcgccag tggtgctgtc gtgatgcggt cttcgcatgg
     8641 accgcacaat gaagatacgg tgcttctgta tcgtacttat tgtttctggt gcgctgtttt
     8701 cccaagtaaa taataaccgg agtctctccg gcgacaattt actggtggtt aacaaccttc
     8761 agagcagcaa gtaagcccga atgccgccct ttagggcggc atattttaga ttatccggtt
     8821 ctgtttaaag tcacgcaaaa aaccacccca gcgacgttca tagaatggcg caatatgttc
     8881 ggtaataaag tggctaattc ctttttcccc ttttttcacc tgacaaatat cgattggttc
     8941 atcgctaggt aatgtatcgg tcgctacact tcccgtcgcc tgaataattt cttcgatatc
     9001 accatcggct tcaatgccaa taagtaaatt aggctgtgcc tcttcgttct ctttaattga
     9061 acaaataaaa gcacgcttca ccggcttaat ggttttaaat aaggtggtga gtgaatcaat
     9121 catttgtgct ggcggctctg cgacttccga taatatcagc gattcaccgc cttccaggat
     9181 ttcctggctg ctcagcggat ttccctctcc accaatcaac aaactgattt cacgcggcat
     9241 aaattcttta ccggtcggca gtttggcatt aaggaagagc gtttcgccaa gggtcatctc
     9301 aaacaatgtg cgaacgggca ttacgacaaa tgcctgttcg tcttcaaccg cctgttgaag
     9361 cgcttctaac gaggtgaaaa aaggaatgac gctggtgccg tcttcttttt cccagtgctg
     9421 taaatcaagc gcactatctt caaccacagc ctcgccctgc gccgccgtac caggcaccca
     9481 gacggtggat tccagtagag tacggaaaaa ggccgggcgg tgcgccggtt cggttgctgc
     9541 tttttccagc aggtcttcaa gttcgttttt tgtttcggac atatgaacca caattcattc
     9601 aacgttcggg gcgcaaatgc ctgatgcgct acgcttatca ggcctacaag ggattcgcaa
     9661 tttgttgaat ttgcagaatt tgtaggccgg ataaggcgtt tacaccgcat ccggcatatt
     9721 agtttacgcc gttaacagat tagctatcgt gcgcacacca agtcccgtag cgcccgcaga
     9781 ccactgttca accggcgctt tacggtaagt cgccgagcag tcgatatgca gccagccttg
     9841 ctgatagttc tcaacaaagt gcgacaggaa gcccgccgcc gtgctcgcgc ctgccggata
     9901 cgccgcgctt ccggtattgt tcagttcggc aaagttagac ggcagctggc tgcggtggaa
     9961 ttccgccagc ggcagacgcc agaatggttc gttctcttgt gaggcactcg ccagcaaacg
    10021 accggcaagc gcatcgtcaa aactgaacag cgcgtgataa tcattaccca gcgcagtttt
    10081 cgccgccccg gtgagggtcg ccgcatcaat gatcatttcc ggtttctgcg cactggcatc
    10141 aatcagacca tcggcaagca ccagacgccc ttcggcatca gtgttcatca cttcaacttt
    10201 tttaccgttg cgataggtga tgatatcgcc cagcttgaac gcattgccgc taatcaggtt
    10261 atccgcacag cagaggaaca gcttcacgcg cttgttcagt ccgcgcgtaa tggcaaatgc
    10321 cagcgccccg gtaaccgttg ccgcgccgcc catgtccgac ttcatcgagt ccataaacgc
    10381 agtctgtttg atgctgtagc cgccggagtc aaaagtgata cctttaccta ccaggcacgc
    10441 gtacaccggt gcttctttat cgccagttgg gttgtaatcc agcgccagca ataccggaga
    10501 acgttctgaa ccgcgcccga cggtgtgcag ccccatataa ccttgctcac gcagatcttc
    10561 gcctttggtg atccgataag tcacacgatc gcccgcgacg ttgctgatca gatcaacagc
    10621 acgctgtgcc agttgcgatg gtcccaattc ttctgccggt gcgttgatgg tgtcacgcac
    10681 ccagtcgatg atcatcaggc ggttatccag ttcctggcgc tgggcatcgt ccagatccgg
    10741 ccactccact ttacgcgtgc ctttcggggc tttgtaacct tgccagaatg cccagcagcg
    10801 atccgcatcc cagccttcac cgcttaactg aacatgcttg atgcccagac cgtcaatctt
    10861 gcgcgccgca cgctggatca gccccagatc gtctgccccg ttcaaatgca gggtaatgcc
    10921 gtcattatta atgctgtaag ttgctttttc tccccagcgc gcgtcggcag gttgggtaga
    10981 gagggtaatc ttcatcgctt ctgtcatttt agttatcctt ctttgtaaaa gggccgccag
    11041 ttggcagccc caggattttt ccgctaaatg attcgcgttg caggaaggcg gcaagtgagt
    11101 gaagccccag gagcatagat aactatgtga ctggggtgaa cgagcgcagc caacgcatct
    11161 gcggcgtgaa gcatgacgcg gaaattattc ggcctcgtcc agccagacta acaaaatcgc
    11221 ttcgaggatt ttctcgttgg atgcctgcgg gtcgtcgtcg aaatcttcca gatcgcaaat
    11281 ccactgatgc atatcggtga atcgaaccgt tttcggatca agatcgggat acgcatcgta
    11341 cagtgcttcg ccaatttcgc ggctatcggt ccatttaagt cccatactaa cctctgttaa
    11401 tgctcacgcg catggttgat agtgtaacgc gggatttcga ccactaaatc ttcgtcggtg
    11461 acgcgcgcct ggcagcttaa acggctttcc ggctccagtc cccaggcttt gtccagcatg
    11521 tcgtcttcct gctctgagct ttccggcagc gagtcaaaac cttcacgaac gatgcagtgg
    11581 caggtggtgc aagcacagga tttttcacag gcgtgttcaa tctcgatacc gttacgcaga
    11641 gctgcgtcga gaatggtttc accgctatta gcttccagaa cagcgccatc agggcagaga
    11701 tcctgatgag gcaaaataac aatctttggc atattaaacc tcgtccacgg aatggccttt
    11761 cagcgcacga cgaaccgact ggtccatgcg gcgagcggcg aaatcctggg tttgtttgtc
    11821 tacgttttta atcgcttgtt cgatggcgtc aacgtcatcg ccctgcgcca cttcactcag
    11881 gtgagcggca gcatcgtcaa tgacctgacg ttctgcggcg cttaacagcg cggcatcagc
    11941 agccagcgcg ccgtgcagac tttccagcac acgcgccgct tctacttttt gttctgccag
    12001 cattcgggct tttacgtcct gctcggcata gctcattgag tctttgatca tcgaagcgat
    12061 ttcgctatcg gtcagaccgt aagacggttt gacctgaata gacgcctcaa cgccggtgga
    12121 tttctccatc gccgtcacgc tcaaaagacc gtcggcatcg acctggaacg tcacgcgaat
    12181 atgcgcacca ccagccggta gcgccggaat accacgcagc gcaaaacgcg ccagtgagcg
    12241 gcagtcctgc accagttcgc gctcaccctg cattacatgg atagacatcg ccgtctgacc
    12301 atctttaaag gtggtgaaat cctgagcgcg ggccaccgga atagtggtat tacgcggaat
    12361 cactttctcc accaggccgc ccatcgtttc gaggcccagc gacagtggga tcacatcaag
    12421 cagcagcatt tcgctgtctg gcttgttacc caccagaata tccgcctgaa tcgccgcgcc
    12481 aatagcgacg actttatccg ggtcgatgga agtcagcggt ggacgaccga aaaattcgcc
    12541 tacccgttca cgcaccagcg gcacgcgagt agaaccgccc accatcacca cttccagcac
    12601 ttcatcagct tctacacccg cgtctttcag cgcgcgacga caagccagta aggttcgttt
    12661 taccagtggc gcgatcagtt cattgaattg ttcacggctg atttcgccct gccagcccgc
    12721 aacgttaacg gtcacggagt ccgcatcgct cagcgcgatt ttggctgcaa tggcggcatc
    12781 cagcagttca cgctgaacgc ggttatcgct acgatcagga atgcccgcct gctcgcgaat
    12841 gtaatccgcc agcagatggt cgaaatcatc gccgccgagc gcggaatcac cgccggttgc
    12901 cagcacttca aacacgccgc gacttaagcg cagaatggaa atatcaaacg tcccgccacc
    12961 gaggtcataa acagcgatca cgccttcctg accggaatcc agcccgtagg cgatagccgc
    13021 agcggtcggt tcgttaagta agcgcagaac atgcaacccc gccagacgtg ccgcgtcttt
    13081 ggtgccctga cgctgggcat cgtcaaagta cgccggaacg gtgataacta caccatccag
    13141 ctcgcctgcc agggcttcag ttgcccgcgc cgccagtgct ttgaggatgt ccgcagaaac
    13201 gcgcaccggg ttcagcagcc ccgccgccgt ttcaatcatc ggcaggccgt tttcgctggc
    13261 ctggaattga taaggcagat gcggatagcg ttgctggata tcagccagcg agcgccccat
    13321 caggcgttta acagaactaa tggtgttggc ggtgtcaagc gctgcgttag tacgcgcgtc
    13381 ataacccacc gaatgccctt gctgttgata gtgaacaaca gatggcagca ggtgacggcc
    13441 ttcatggtcg gctaacgttt cggcctgacc gctgcgcact gtcgccacca gcgagttggt
    13501 tgtgcccagg tcaataccag ccgccagacg acgctgatgc ggcgcagcac tcaaaccagg
    13561 ttcactaatt tgtaataagg ccatgtttag cttccagaaa ttaaaaatcg agcagttttt
    13621 cttcgagttg ttcggcactg cttcgcagtt tatcgagaaa acgcagctta cgcacggtat
    13681 ccgccgccgc gtcccacgtc tcgttgtcta actgttcaac catcaactga tggcgggtat
    13741 caaacatctt tttcacacgt ttgataaagc tttccagccg cgcttcatct ttcgcctgtt
    13801 cgatctcgtc cagttcttcg cgcagctcca gctgttccat caggaacgcg gtgtcgcgca
    13861 cagtatgctg ctcgctggcg agatcaaaac cgtgcaaaga aagcaaatat tccgcgcgca
    13921 ttaacggatg acgcagcgtt tgccaggcct ggttaatggt tgcagattgc tgtacggcgg
    13981 cgagttgttc cgcctggctt ccgctggcga atttatcagg atgatactga cgttgtagat
    14041 cctgaaaacg caggctcagt gcctgggtat cgagttgata acgggcaggc aagccaaaga
    14101 gggtgaagta atccataaca ttctcagggc tgcgatccgc cgcgataacg gcgaatcagg
    14161 taaaacaaac cccacgcgca ggcgaccacg gtggggttat cggtatgcgc atcaaacgtg
    14221 gaagctttcg ccgcaaccac actcatcttt gacgttcggg ttggtgaatt taaacccttc
    14281 gttcaggcct tcttttacga agtccagttg cgtaccgtcc agaaattgca ggcttttgcc
    14341 atcgaccacg actttcacgc ctttgtcttc aaacacgatg tcttccggcg tcggttcgtc
    14401 aacaaattcc agtacataag ccatacctga acatccggag gttctcacgc ccagacgcag
    14461 gccaaaccct ttgccgcggt tagccagaaa ggtatttact cgcgctgctg cactgtcgct
    14521 cagtgtaatc gacataacca aacctcaact cttattttgc ttcacgtttg cttttatagt
    14581 ccgcaatggc ggctttgatc gcgtcttctg ccagaataga acagtgaatt ttcaccggcg
    14641 gcagttcaag ttcttcagca atatcggtgt ttttgatcgc ctgcgcttcg tcgagagact
    14701 tccctttcac ccattcggtg accagggagc tggaagcgat agcagaaccg cagccgtaag
    14761 ttttgaaacg cgcgtcttca atgatacctt catcgttgac tttaatctgc aacttcatca
    14821 cgtcgccaca ggccggtgca cccaccatgc cgctgccgac gttctcgtcg ttgttgtcaa
    14881 aggaacccac gttacgcgga ttctcgtaat ggtcgataac tttttcgctg taagccatta
    14941 taaattctcc tgattccgat accgattaat gatgagccca ttcgatgcta ttcagatcca
    15001 cgccctgctt gtacatttcc cacagcggag aaaggtcacg cagacgaccg atggatttac
    15061 gaactaactc gatggtgtag tcgatctctt cttcagtagt aaaacgacct aaagagaaac
    15121 ggatagagct atgtgccagc tcgtcgttca gccccagcgc gcgcagcacg taggacggtt
    15181 cgaggcttgc tgacgtacag gcggagccgg aagaaaccgc gaggtctttc agcgccataa
    15241 tcagcgactc accttcaacg tagttgaagc tgacgttgag aatgttcggc gcaccgtgtt
    15301 ccaggtcacc gttcaggtaa acttcttcga tatctttgat gccgttccac agacggttac
    15361 gcaggccgcg cagacgttcc atctcggtcg ccatctcttc ttttgcgatg cgataggctt
    15421 cgcccattcc gacgatctgg tgaacaggca gagtgccgga acgcataccg cgctcgtgac
    15481 cgccgccgtg catttgcgct tcgatgcgta cacgcggttt acgacgcaca tacagcgcac
    15541 cgataccttt cgggccatag attttgtgac cggagaaaga catcaggtca actttcaact
    15601 ggctcaggtc gattggcagt ttgcccacgc tctgggttgc atcaacgtga tagataatgc
    15661 cacgagcacg gcacatttcg ccgatagccg cgatatcctg caccacgccg atttcgttat
    15721 ttacgtgcat gatggaaaca aggatggtgt cgtcacgcat cgctgcttca agttctttca
    15781 ggtcgataat gccgttacgc tgcggtgcca ggtaggtgac ttcaaaacct tcgcgctcca
    15841 gctgacggca ggtatccagt accgctttgt gttcggtttt gctggtgatg atgtgcttgc
    15901 cttttttctg ataaaagttg gctgcacctt tgatcgccag gttgtcagat tcggttgcac
    15961 cagaggtaaa gacgatttca cgcggatcag cgccgaccag atcggcaatc tgattacggg
    16021 cgatatctac cgcttcttca gcctgccagc cgaaacggtg agaacgagag gccgggttac
    16081 caaaggttcc gtccatcgtc atgaactgca tcattttctc ggcaacacgc gggtccaccg
    16141 gcgtggttgc ggagtagtcg agataaatcg gtaatttcat tgctctataa actccgtaca
    16201 tcactcaatg caaggaacca ggctaccggc tggatgtacg accgtgttta cggagtattt
    16261 agcactccgg cctgattctg aattcttttt attaagcgcg taacttaacg tcgatcgcgt
    16321 cttgtgtgcg ggtgcgtggc gcgtcgtgag tatgctgacg accagacaca tccagcactt
    16381 cctggttatt aaccagttcg cctaaagtaa tgttgttgag aaaaccggtg agacggtcgc
    16441 tcaaatcacg ccacagcgcg tgggtcaggc atttatcgcc gccctggcag ccgcctttac
    16501 cctgacaacg ggtggcatct acagattcgt caacggcgct aatcacttcg ccaacggcga
    16561 tgctgctggc atctttgcct aacagataac caccgcctgg tccacgtacg ctggaaacca
    16621 gaccattttt acgcagacgg gaaaacagtt gttccagata agaaagggaa attccctgac
    16681 gttcggaaat atcagccaac ggtaccgggc ccgcttcaga gttgagcgca acgtcaagca
    16741 ttgcggtcac ggcatagcgc cctttagatg tcagtctcat gtcttacttc acctcaaact
    16801 cgcccctgcc cggggttttt tattgtaaaa gtgggggtat tgcatagcag ggtcaagtct
    16861 gacattcccg agtaaattgg tcaactattt acttgactga tttagtcggg tatttaacct
    16921 tcagtgccat ttttttatcg tggcgtttgt acctgttgtc cttccagacc atgggcgggt
    16981 tgagggcaat tattcgcatt agcgctggcc tcttcccctc accctaaccc tctccccgaa
    17041 ggggcgaggg gaccgtccgt gcatttatct ggcttagccc tcaggcctta ttacctttat
    17101 tctgctgctc aatagaagcc agaatcccgc gcaggatatt caattcctgg ctttccgggc
    17161 gcgcacgggt aaacagacgg cgcaatttat tcatcacctg ccccggatgg ttttcacgga
    17221 taaaaccggt tgccagcagc gtttgttcca gatgaccata aaaacgctcc agatcatcga
    17281 ccagcggata cggcgtctct tcatgctcga cctgctcgcc gttttcctga gttgccagcc
    17341 aggccatgcg cacttcatag gcaataacct gaaccgccat cgccaggttc agcgagctgt
    17401 attccgggtt agccgcaatc gcgacatgat aatggcattt ctgcaactct tcattggtca
    17461 agccgacgcg ctcgcgacca aacaccagcg ccaccggggt atttgccgct tcagcgacgc
    17521 ttttcaggcc gcattcgcgc gggtcgagca tcggccatgg cagcgtgcgg gagcgtgcgc
    17581 tggtgcccac caccaggcta caaccggcta acgcttcgtc gagcgtatcg acgatgtgag
    17641 cattaccaat cacatcgctg gcccctgctg ccagggcaat cgcctgggag tcgggtttca
    17701 ccagtggatt aaccagccac agattggtta atcccattgt tttcatggca cgggcaacag
    17761 aacccatatt gccggtgtgt gacgtctcca ccagcacaat tcgaatattt tgcagcattg
    17821 tctttcttca tctaaagatt attcacgcat cttatcataa aacgaagaca gatgccgatc
    17881 tcgctgctat actctgcgcc gttttcccgt tctttaacat ccagtgagag agaccgatgc
    17941 atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc gggtaattta attgccaaaa
    18001 actatgaaac cccggacgct gtagaagcga gccagaaagg cagtaacgat ttcgtgacca
    18061 acgtagataa agctgccgaa gcggtgatta tcgacacgat tcgtaaatct tacccacagc
    18121 acaccatcat caccgaagaa agcggtgaac ttgaaggtac tgatcaggat gttcaatggg
    18181 ttatcgatcc actggatggt actaccaact ttatcaaacg tctgccgcac ttcgcggtat
    18241 ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt ggtatacgat cctatgcgta
    18301 acgaactgtt caccgccact cgcggtcagg gcgcacagct gaacggctac cgtctgcgcg
    18361 gtagcaccgc tcgcgatctc gacggtacca ttctggcgac cggcttcccg ttcaaagcaa
    18421 aacagtacgc cactacctac atcaacatcg tcggcaaact gttcaacgaa tgtgcagact
    18481 tccgtcgtac cggttctgcg gcgctggatc tggcttacgt cgctgcgggt cgtgttgacg
    18541 gtttctttga aatcggtctg cgtccgtggg atttcgcggc aggcgagctg ctggttcgtg
    18601 aagcgggcgg catcgtcagc gacttcaccg gtggtcataa ctatatgctg accggtaaca
    18661 tcgttgctgg taacccacgt gttgtgaaag ccatgctggc gaacatgcgt gacgagttaa
    18721 gcgacgctct gaagcgttaa tgactcaggc gggtgatgtc actcacccgc cctcgccttt
    18781 caggcgctat tctgaaatac ttcctcaccg ctttactttc tttcgtcact ctcccaccat
    18841 ctttccccga ttaatggatg ataaagaagt cgcaaggaaa ggttatggca ctgccagtga
    18901 acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt tgccttctgc gtttatttag
    18961 tgccgcgcgt tgccatcaac ttcttctatt atcctgacga taaaatttac ggtcccgacc
    19021 cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg tactcgtctg caaggctggt
    19081 ttattccttc ttcgacgggc cctgctgaca acgccatcgc aaccatcatt catgctcacg
    19141 gcaatgccgg aaatatgtcc gcccactggc cgctggtcag ttggttaccc gagcgtaatt
    19201 tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc aaaaggcacg ccgtcccagg
    19261 ccggattgct ggacgatacg caaagcgcca tcaatgtggt gcgccatcgc agtgatgtaa
    19321 acccacaacg gctggtgctg ttcgggcaga gtattggcgg ggcgaatatt cttgctgtta
    19381 ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct cgactccaca tttgcctctt
    19441 atgcaaccat cgccaaccag atgatccccg gcagtggcta cttacttgat gagagttaca
    19501 gcggcgaaaa ttatatcgcc agcgtcagcc cgatcccgct tttactcatt cacggtaaag
    19561 ctgatcacgt tatcccatgg cagcacagcg aaaagttgta tagcctggca aaagagccaa
    19621 aacggttgat cctaatcccg gatggcgaac acattgatgc tttttccgat cgccacggcg
    19681 atgtttatcg cgaacagatg gtggacttta tccttagcgc gttgaatccg cagaactaac
    19741 ccatgatcgc tagcacgata atcattcaca aaaccacctt aagacatgct aatccactgg
    19801 tcagaacagt ttaagatgag aaaaattctg tgacgcttgc caacatttct gatgattagc
    19861 attcccttcg ccatttccct gagcaaactt tagctattct tatcaattat gcttatggga
    19921 gatctacaga tgatgcctac gcttgctcca ccatctgtcc tttcggctcc ccagcgccgc
    19981 tgccagatct tgctgacgct ctttcagccg gggttgaccg ccaccatggc aaccttcagc
    20041 gagcttaatg gtgtggatga tgatattgcc agtcttgata tcagcgaaac aggacgggag
    20101 atcctgcgct atcatcaact cacactgacg actggttatg acggtagcta ccgggttgaa
    20161 ggtacagtgc ttaaccaacg tttgtgttta tttcactggc tacgacgtgg tttccgtctg
    20221 tgtccgtcat ttattaccag ccaattcacc cccgccctga agagtgaact gaagcggcgc
    20281 ggaattgcgc gtaactttta cgacgatacc aatctacaag cgttagtgaa tctctgctcc
    20341 cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt tcctgtgtct gtatctgcaa
    20401 tattgtttgc tgcagcacca cgctggaatt acgcctcagt ttaatccgct ccaacgtcgc
    20461 tgggcggaat cctgccttga atttcaggta gcgcaggaaa ttggacgcca ctggcagcgt
    20521 cgggcgctcc agcctgtacc gcctgatgag ccactgttta tggcactact tttttccatg
    20581 ttgcgggttc ccgatccact gcgggatgcg catcagcgag acagacaatt gcgtcagtct
    20641 atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg ttcgttttta tgatgaacag
    20701 gggttatgtg atcagcttta tacccacctc gcccaggcgt taaatcgcag tttgtttgcc
    20761 atcggtattg ataataccct gccggaagag ttcgccagac tgtacccacg cctggtgcgc
    20821 accacccgcg cggcgctggc cggatttgaa agtgaatacg gcgtccatct ttctgatgag
    20881 gaaagtggtc tggtcgcggt gattttcggt gcctggctaa tgcaggaaaa cgatctgcat
    20941 gaaaaacaga ttattctact gaccgggaat gatagcgagc gagaagcgca gattgagcag
    21001 cagttacgcg aactaacgtt actgccgctc aacattaagc atatgtcggt aaaggcattt
    21061 ttgcagacag gcgctccgcg tggcgcggca ctgattattg cgccttatac catgccatta
    21121 ccgctctttt caccaccgct gatctatacg gacctgacgc tgacaacaca tcaacaggag
    21181 cagatccgca aaatgctcga atcagcatga gggaacaact ttcgggcgca aaaacattgc
    21241 cggcagcgcc accagcgcca ttacccagaa cacgccgtgg cccagatatt gatacaggaa
    21301 accggcgaaa acggtcatga tagcgatact gccgcccatc gcgacggcag agtaaaccgc
    21361 ctgtaaacgg atgacttcgc taccctggcg agcagcaata taacgcatgg cggccaggtg
    21421 gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact atcaaccacg gcaacgccgt
    21481 agttgctccc ataatgcccc agcgcactac gccgcaaatc gccgagatca acagcatatc
    21541 gcgtgcacta caacggcgga aaagtttatt actcagcgca aagataatga cttccgccac
    21601 cacgcccagc gaccacaaat accccaccgc cgaggccgag tagccagctg cctgccagta
    21661 aatggcgcta aaaccgtaat aggccgcatg tgccccctgc aataaacaaa cgcaggccag
    21721 aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac caaccggtgc tctcctgctg
    21781 gcgacttgcc ccttgtggct gaatcgtcgg acggatgaga aagccgagca gcatggatgc
    21841 cacgcccaac gtcaacagcg cgaggatcac ccgataatca aacatagtga ccagtttgcc
    21901 cgtcagcgcc gagccaatga caaacgccac cgagccccac agtcgcactt tgccgtaatc
    21961 aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg gtcaacggta ccagtggtga
    22021 gaaaaagagg ttaaagccaa tcatcaccag catcagccac gctacgtgcg cccccgccca
    22081 gaaggcgaca gcaaagagaa gtgtcagcag tgccagcacg cgcaaggcgg aaatcaggcg
    22141 ggaaggatcg ctgacgcggg gcgcgatgag caaactcccg aggaaacggg caaccagacc
    22201 tgcccccaat aacaggccga tggtttctgg cgttaaacca atccctttaa gccagacgct
    22261 ccagaaaggt agaaaaatgc cgtaactaaa aaagtatgtg aaatagccga gcgccaacca
    22321 gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag gcgttagtct ggggttaatc
    22381 gaatgttggt gcaagttgaa aaataagaat attcgagcct gcttcccgat gtctgtaacg
    22441 ccagttcaca agcactaaaa aactgagatc cgcgacgcat gttgcaaaaa taatgcgtaa
    22501 ttatgccgtt acgcttgcca aacgttcctg cacaatggcg atgaaatcgc gtaatgccgg
    22561 cttcatctcc cctttcttcc acgccatcag taaagcaatg gaaggcacat ttccggcaat
    22621 gggacgaaaa acaacctgtc cggtattaaa attattcata taaccgggta tcaaagtgac
    22681 gcccagcccc atgcccacca gattcatggt caccagaata ttcgttgcca cctggacgat
    22741 atttggctgg ctgttttctt gcgcaaacca cgctttaacg atcggcgcaa gcgaaccgga
    22801 atacaccgga tcggtactga cgaaattcac gccatccagt tgcgcggcgg tgatctcttt
    22861 ttcatgtgct aaagggtgat caaccggtaa cacaaccact aatggttcgt caaaaagctc
    22921 cagataatca atctccgggc tataaacagg atggcgcatt aagccgacgt cgagttcacc
    22981 acggcgaatt ttttcctcct gttgcgtggt gattaaactc accagctcaa tcaaggtgtc
    23041 tggctgtctg agacgaaaca tcggtaatac ttttggcagt aaattcactt ccgccgatgg
    23101 cacaaagcca atggttaatt gtctgtcttc ctgaacaatt ttccgcgccc gtaatttggc
    23161 attttccgct tgttcgagga ttgccagcgc gtcctggaga aaacattctc ccgccgccgt
    23221 caacgcgact ttgcgcttat ccctcaccag taacggaaca ccgacacagt tttcaagatc
    23281 gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt ttttccgccg cacgggtaaa
    23341 gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc cgtagttcca tcaccttccc
    23401 cttgttatcg aaaaaacgtc tcagctggta gaaattaact atttcacata ttagcaacca
    23461 accagcaaca tccttatggc acaaaaatag aaggtcaata catcttatct ttcaggatta
    23521 aaaaatatga ccacaccctc agatttgaac atttaccaac tgattgacac ccaaaatggt
    23581 cgggtcactc cgcgtattta taccgacccg gatatttacc aactggagct tgagcgtatt
    23641 ttcggtcgtt gctggctatt tctcgcccac gaaagccaga tcccaaaacc cggtgatttc
    23701 tttaacacct acatgggaga agatgcggtt gtcgtagtgc gtcagaaaga cggcagcatc
    23761 aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg tgagttatgc agattgcggc
    23821 aacactcgcg cctttacctg cccgtatcac ggctggtctt atggcattaa cggcgagttg
    23881 atcgatgtac cgctggaacc tcgcgcctac ccacaagggt tgtgtaaatc ccactgggga
    23941 ctaaacgaag ttccttgtgt ggagagttat aaagggctga tttttggcaa ctgggatacc
    24001 agcgcaccgg gcctgcgtga ttacctgggt gacattgcct ggtatctgga tggcatgctg
    24061 gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac aaaagtgggt gatcaactgt
    24121 aactggaaat tcccggcaga gcagttcgcc agtgaccaat atcatgctct gttcagccat
    24181 gcttctgccg ttcaggtatt aggggcgaaa gatgatggca gcgataagcg cctcggtgat
    24241 ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg cgctgcaatt tggtcaggac
    24301 ggtcacggta gcggtttctt ctttactgaa aaaccggatg ctaatgtctg ggtcgatggc
    24361 gcagtttcaa gctattaccg cgaaacctat gccgaagcag aacaacgttt aggtgaagtt
    24421 cgcgccctgc gcctggcggg tcataacaat atattcccca cgctttcatg gctaaacggc
    24481 actgccacac tccgcgtctg gcatccgcgc ggccctgatc aagttgaagt gtgggcgttc
    24541 tgtattactg acaaagccgc ctccgatgaa gttaaagccg cttttgaaaa cagcgccact
    24601 cgtgcttttg gtcctgctgg ttttctcgag caggatgact cggagaactg gtgtgaaatc
    24661 cagaaattgc tgaaaggtca ccgcgcccgc aacagcaaac tgtgtctgga aatggggctt
    24721 ggtcaggaaa agcgtcgcga cgacggcatt ccaggcatta ctaactatat cttctcagaa
    24781 acggccgctc gtggaatgta ccaacgctgg gccgatcttc tgagtagcga aagctggcag
    24841 gaagtgctcg ataaaaccgc cgcttaccag caggaggtga tgaaatgagt gcgcaagttt
    24901 cactagagtt acatcaccgc attagccagt ttctctttca cgaagccagc ttactggacg
    24961 actggaaatt tcgtgactgg ctggcgcagc tcgacgaaga gattcgttac accatgcgca
    25021 ccacagttaa cgcgcaaaca cgcgaccgcc gcaaaggcgt ccagccaccg acaacctgga
    25081 tttttaatga caccaaagac cagctggagc ggcgaatcgc ccgtctggaa acgggcatgg
    25141 catgggcaga agagccgccg tcacgcaccc gccacttaat cagcaactgc cagataagcg
    25201 aaaccgacat cccaaacgta tttgctgttc gggtgaatta tctgctttat cgggcacaaa
    25261 aagagcgcga tgaaacgttc tatgtcggga ctcgttttga caaagttcgc cgtctggaag
    25321 atgacaactg gcgcttgctg gaacgggata tcgtcctgga tcaagcggta atcacttccc
    25381 ataacctgag tgtactgttc tgatgaatcg aatttatgcg tgtcccgttg cggatgtgcc
    25441 ggagggtgag gctctccgga tcgatacctc gcccgtcatc gccctgttca acgttggcgg
    25501 cgagttttat gccattaacg atcgttgcag ccatggtaat gcgtcaatgt cagaagggta
    25561 tctggaagat gacgccacgg tggagtgccc gctacacgcc gccagttttt gcctgaaaac
    25621 ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc acttatccag tacacgttga
    25681 aggtggtgac attttcatcg acttaccgga ggcgcagcca tgagcgatct gcataacgag
    25741 tccattttta ttaccggcgg cggatcggga ttagggctgg cgctggtcga gcgatttatc
    25801 gaagaaggcg cgcaggttgc cacgctggaa ctgtcggcgg caaaagtcgc cagtctgcgt
    25861 cagcgatttg gcgaacatat tctggcggtg gaaggtaacg tgacctgtta tgccgattat
    25921 caacgcgcgg tcgatcagat cctgactcgt tccggcaagc tggattgttt tatcggcaat
    25981 gcaggcatct gggatcacaa tgcctcactg gttaatactc ccgcagagac gctcgaaacc
    26041 ggcttccacg agctgtttaa cgtcaatgtt ctcggttacc tgctgggcgc aaaagcctgc
    26101 gctccggcgt taatcgccag tgaaggcagc atgattttca cactgtcaaa tgccgcctgg
    26161 tatcctggcg gcggtggccc gctgtacacc gccagtaaac atgccgcaac cggacttatt
    26221 cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga atggcgtcgg cccgtgtggt
    26281 atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa gtgaaacctc gataatgcag
    26341 tctctgacgc cggagaaaat tgccgccatt ttaccgctgc aatttttccc gcaaccggcg
    26401 gattttacgg gtccgtatgt gatgttggca tcgcggcgca ataatcgcgc attaagcggt
    26461 gtgatgatca acgctgatgc gggtttagcg attcgcggca ttcgccacgt agcggctggg
    26521 ctggatcttt aaggaagcac gatgaaagaa aaaaccatca ttattgtcgg tggcgggcaa
    26581 gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt tcaccggtga gctgcatctg
    26641 ttttccgatg agcgacatct tccttatgaa cgacctccac tctcgaaatc catgttgctg
    26701 gaagattccc cgcagttaca gcaggtgtta cccgctaact ggtggcagga aaacaatgtt
    26761 catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg acacacgaga gttagtgtta
    26821 accaacggcg aaagctggca ctgggatcag ctttttatag caaccggcgc ggcagctcga
    26881 ccgctgccgt tgcttgatgc actgggagaa cgctgcttta ccctacgcca tgccggtgat
    26941 gccgccagac tgcgagaagt tctgcagccc gaacggtcag tcgtgattgt cggtgccgga
    27001 actattggtc tggaactggc tgccagcgcc acgcagcgca gatgtaaggt gacagtgatt
    27061 gaactggcgg caaccgtcat gggccgtaat gcaccaccgc ccgtgcaacg ctatctttta
    27121 cagcgccacc agcaggctgg tgtgcgcatt ctgctcaata atgccattga acatgtggtc
    27181 gatggtgaaa aagtagaact gacgctgcaa agtggggaaa cgcttcaggc tgatgtggtg
    27241 atttacggta ttggtatcag cgccaacgag caactggctc gcgaggccaa ccttgatact
    27301 gccaatggca ttgtcattga tgaggcttgc cgcacctgcg atcccgcgat ctttgccggt
    27361 ggcgatgtgg caatcactcg tcttgataat ggtgcactac accgctgcga aagctgggaa
    27421 aacgccaata accaggcgca aattgccgct gccgcaatgt tagggctacc gctaccgcta
    27481 ctgccgccgc cgtggttctg gagcgatcag tacagtgata acttacagtt tattggcgat
    27541 atgcgtggcg atgactggct ttgtcgtggc aacccggaaa ctcagaaggc gatttggttt
    27601 aatctgcaaa acggcgtgct tatcggtgcg gtcacgctga atcaggggcg tgagattcgc
    27661 ccaattcgca aatggatcca gagcggcaaa acgtttgatg cgaaactgct gatagatgag
    27721 aacatcgcgc ttaaatcact gtaaccagga taattagcga atatctcaat gcctggggcg
    27781 tggcgaggtg caagagtgtg tattacgttt aaatcacatt atcttgcaaa gggattggtt
    27841 atgaacacac tacgttattt tgattttgga gctgcccgcc ccgttttgtt attaattgcc
    27901 cgtatcgccg tggtcttaat tttcattatt tttggttttc ccaaaatgat gggctttgac
    27961 ggtacggtcc aatatatggc ctcgttgggc gcgccgatgc cgatgctggc agcgattatt
    28021 gcggtagtta tggaagtgcc cgccgcgata ttaatcgtgc ttggcttttt cacccgtccg
    28081 ctggcggtgc tgtttatttt ctacacgctg ggtacggcgg tgattggtca ccattactgg
    28141 gatatgaccg gcgatgcggt tgggccaaat atgattaatt tctggaagaa tgtcagtatc
    28201 gctggcgcat tcctgctgct ggcaattacc gggccggggg caatttctct cgatcggcgt
    28261 taggaaagat gccggatgcg gcgtgaacgc cttatccggc atttaataaa ttacagccac
    28321 tctacccgca acgacatctc tgaagttgtt gattcccctg gtgctagcgc aatgagatca
    28381 cccccttccg ggcgatgatg atcatccggc gcatggctca tcggttccag acagaagaaa
    28441 tcaaacgcat atcctttatc aaacgcaggg tcggaaacaa agatgaaata acacggtgca
    28501 ggtggcgtcg tttccatgat gatggcataa ccttcctgcg gctgctcgat gcgggcctga
    28561 ccattccatc cggcaaaacc attgttcacc cactggcgcg gcaacggcgc gggctggtta
    28621 aaatccagtt cctgcggtag ctgctcgcaa aactcacccg ccagccactg ctcccgctcc
    28681 agccagtaac cgctcgcctg tgcctgaatc cgcgtttgcg gcgacaacgg aaaataagga
    28741 tgccagccgg taccaaatgg cagcgtctct gccccttgat tggtgacaga gagcgtcacc
    28801 gtcagcgtat ccgccgttaa atgaaacgcc tgacttaccc gatagtgata gacaccgctg
    28861 cgatgttcat acaccagaca caaactatca tcgctgtgcg agacacattg ccactcgccc
    28921 agccagccat cgccgtgtag atagtgcgca tcccactcaa cattcggttg cagttgatac
    28981 tcacgcccct gccagacaaa acgattgccg ctcacccggt ttgcaaatgg caccagcgga
    29041 aaacatgagg catcggttgc cacaccgctt tttttaccag gacgtaagag cggcgtcgta
    29101 tcgcgccaga agccttcgat tacgccgccc tggtcagaaa cgtccagctt tagcgacccg
    29161 tgggataagg tatagatggt catccgctaa ctccttaatc cgggaagtta atcacaactt
    29221 tcccgcattt gccgctcgcc atcagcgcat aggcatcacc tgcctgttcc agcgagaagc
    29281 gatgggtaat ggcgttacgc ggccacagct tccagtccgt cagatcatgg gcgcattttt
    29341 ccatatggaa cagactggtc acccaggagc cgataatccg ccgttgatgg tgcatcagat
    29401 cggcgctgac ctcgaattcc acttttccgg tttcaccaat gtaaaccacc cgtccccagt
    29461 cagcggtgga ttgcagtgcc agcaagcgac ctgcggcatt accggaacaa tcgagcgcaa
    29521 catccgcgcc accgtgggtg agttcggcga taatctgcgg cagaccttcg gtggttgcta
    29581 aatagccgtg atccatcacc cctaactgtt ttgccatcgc cagacgttcc ggcagcatat
    29641 caacgccgat gatccgtttt gcaccgcgac ctttcgccag catcatcgcc atcatgccga
    29701 ccggccccag accgaccacc agcacgttat cgctgccgga aacttcgccg cgcaaaatcc
    29761 cttcataggc cgtgccaacg ccgcaactga taaacgcacc atcttcgtag ctcagcgcat
    29821 ccggcaggag gatcagatct ttttcttccg ccagcagata ttcggcatga ccgccgtcac
    29881 gctgccagcc gtaagccgct tttcctttgc cagtacaaga gataggaaag ccgcggcggc
    29941 agttcgggca aaaaccacag ccagaaatgt gatacaccag cacgcggtcg ccctctttaa
    30001 aatggcggca gccttgcccc atcgccacaa tctgcccgca cggttcatga ccgttgataa
    30061 atccctggta taacggtttg tcgggtgccg ctgccgtagc gcggtgctgg tggtagatat
    30121 agtggacatc gcttccgcaa attccggaag atttcatttt gatcagtacc tggttaatcc
    30181 ctggtgtcgg caccgcaact tcccgtagat cgacggtcga atttcctggt aaataagctg
    30241 ccagcatcgt tttcataaat ccctcattaa caatacgatt aattttcatc cctgccgcac
    30301 ccgcgccagg ggcgttaagg tttagcgttt cgctttactg cttcgctggg tcagcaagat
    30361 attcgccagc accgccacca cgatgatgac gccgcgtacc acctgctgga aaaaggagtt
    30421 aataccgagc agcaccagac cgttaccgat tagcgtaatc accagcacac caagcaatgt
    30481 accgaacagg gaaccgcgac cgccggaaag tgccgtaccg ccgaccacga ccgcggcgat
    30541 gacgtcaaac tccagaccgt ttgcggcacc tgcgttacca gaaccgaggc gcgccgccaa
    30601 caaaatgccg gtcaccgccg ctaataatcc cgaaagggta aagataagaa tgcgcacccg
    30661 acgaacgttg atgccgcaca actgcgccgc cgtagcatta ccgccaacgg caaaaaccga
    30721 gcgcccgaag gcggttttgc ggctgatgaa cacaaacagc gcaaacaaca caatcatgat
    30781 cagcgcggat accggcacac cgagaaattg tccgcccagc cagtccagca cctcgttttc
    30841 gtcaatcggc actggcagtg cgttcgtcat aaacagcccc attccgcgca gggcgctcca
    30901 cagccccagt gtggcaacga aacttggtac gttaaacacg ccgcgcagca ccccggcgag
    30961 cgttcccatc agcgcgccta acagcaacac cagcagacaa gcaatcgcca acggaacttc
    31021 aaattgcagc aaaaatgcca ggcacaccga gacaaaagcc accatcggcc caacgctgac
    31081 atcaatctca ccggagataa taatcagcgt catcgcccag gcggcaatcc caatggtggc
    31141 ggcatcgcgc agcacgttca tctggttatt caatgagata aagccaggcg cgttcaggga
    31201 gaagaccaga taaagaatgg caatcaccac cagcaaaccg atctcattaa tatggcgact
    31261 gacaaattgt ttgagcgaga cgctcttgcc ctgcggcaat ggtaatgacg aagcagacat
    31321 cttcaggttc ctcggtaaaa ttcatcagtg cacagacaga atggcggaca tcagctcatc
    31381 cacattgacc ggcgagtgaa actcctgcga gaacgtaccg tgctgtaaca acaggatgcg
    31441 gtcgcacacc agcggcaact cctccacttc actggagata aacaccacgc tttttccttc
    31501 ggcagccagc tcacggacaa tacggtaaat ctgctgtttg gcttcgatat cgacgccgcg
    31561 cgttggctcg tcgagcaaca aaatctggct ggcggcatag acccaacgac cgatcaccac
    31621 tttttgctga ttgccgccag aaagcgtgcc gatgggcgtt tcgctactgg cggccttgac
    31681 cgtcatccgc tgcatcacct cttcggtcag gcggcggatg gtggaccatt gcagtacgcc
    31741 gttggcgctg attttttgcc gattggtcag cactgtattt tcgtcaacgc ccaaccaggg
    31801 aatgatcccc gcttctttgc ggttttctgg cgtatagcca atgccgcgtt tcagcatgtc
    31861 gccgtaatcg gggcgcgtga ttttctcgcc gttgataaca atttcgccct gttcatactc
    31921 ctccagccca acaatcgcct tcagcaattc actgcgccct gcccccagca ggccagcaat
    31981 gccaagcact tcgccgcgac gtaacgtaaa gctgatatcc tccagcttgg gcttatggcg
    32041 taatgcacgg acttccagca cggcctgatc gacaatttcc tgaggggcta ccggcgcaat
    32101 atcaacgtga tcgcgcccta gcattagcga cacaatatga tgcgtggagg tgttttcgag
    32161 catcacatcg cccgccacct gaccatcgcg cataacggtg gcacaggagg caatgcggcg
    32221 aatttcttcc atccggtggc tgacataaat caccgccacg cccagtgccg acatcttttt
    32281 caccgcgctg atcaccagtt caacttccgc actggcaagc gaactcgtag gttcatcaag
    32341 aatgaccacg cgcggctcgc ccttcatcac ccgtgcaatt tccaccagtt gcttttgcgc
    32401 cgggcttagc gttgaaacaa gttgttcagg actaacgtca acgcccagcg cctgtaagca
    32461 acgttgggca tcctgcgcca tttgcaggta atcaatcatg ccgttgcggc ggggccactg
    32521 accgaggcag aggttttccg ccaccgtcag cccttccacc agacttaact cctgataaac
    32581 cgcacgaacc cccagttcag cgacacggcg agtcaacgta gcttcgtcac cttccagtcg
    32641 cgtctcgcca atccagatat caccgctatc cgggcgttca ctgccggtaa gcattcgaat
    32701 gagagtcgat ttgcccgcgc cgtttttacc taacagcgca cgaacttcgc ctttattgag
    32761 cgtgaagtta acgttatcca gcgcaacgac gccggggtaa cgcttatttc ctgccaccac
    32821 ttttgctacc gggactgcct ctgttgccgt gaacataggg acctctgcga atcagcgatt
    32881 agggcagacc atcaacgtgc gttgccagcc actgtttacc atcttccgtt ttggtataga
    32941 gatcgattgg cacctgaatc actttttcac cgtcggcttg tttattgata accttcaatg
    33001 tttgcgcgag aacagcattg cccattttct taccggaaat atccactacc gctttcagca
    33061 cctgattgtt ttccagctcc tgagcaattt cagtggtcat atccgaaccg aaaacagcaa
    33121 tttttccggc ctgattttga ttacgtaccg cttttaccgc gccgagtgtc gctccgcccg
    33181 attcacccat aatggcgttg agatccggcg tggagataat tagtttttca ccaacggaaa
    33241 tcgctttatc taaaacagtc ccttcctgat tagcgacaat ttgcgcgccg ggaacgtggg
    33301 attttaatac ttcttcaaat cctttacgtc gctgcacaca aacttcaaag gcttcgcaat
    33361 tgatgacggc aattttcggc tggtcaattt tattggcaat aaaataatcg gcggcagcat
    33421 tacccagttt tttaccaaat tccagcggat cgccgaccag atacgccgag acatatttat
    33481 cgacaccctt ttgattaata caggtgttgt agcaaatcac cggaataccc gcttcactgg
    33541 cgcgacgaac ggtacggcta ctgccatttt cagacactgc cgataaaata atggcatcga
    33601 cattacgcgc cacgagggta tcaacaaagg tactttcttt cgaaatatca ccctgggcgt
    33661 tagtttcaat taactgtacc tgtactgaag aatcttttgc cgcatcctga acgccttgac
    33721 gcactccagc gtaatatccc tgagtatcaa ggtatattgc gccaatggtc atttcttttt
    33781 ccgcagccct ggcaaataaa gcgctaccta atagcgtagc cattaataat agattacggg
    33841 ttgttctcat ttttttaggc atagagcctc ctgtagggtt tttattaaca acggcttatt
    33901 ctaattattt tgtgatgagc ggcagcgcgt gccgctcaaa tattacagtt aatagggttt
    33961 agtgaacgta gttaaaaata aaaggcatca cagtggtgaa taatgccgct ttcggccagg
    34021 ccggattaat cgccgtcaat tcagcgcgag cagactcata ttgcgcaacc tccccgagtc
    34081 ccgcggacgc cagcatcgcc accatcaggc atttttcttt atgctgctgt tgtaaatcgc
    34141 catacagcga caacagatca ggttgtgaaa cagcaaagaa atccgcctca atactggttt
    34201 tcgccatctc ttgcgcccac tgtttcattt cactaaacag ttgctgtgcg gtgtgttgtt
    34261 cgcccagcag tcgcagcgcc attccttgcc agaagagata atcaaccggc tgatcgttgt
    34321 aataactgtg gatgttaatg gtgcgatcgc cggtcgccgc cagacgtaaa caacgcatcg
    34381 cttcagtttc atcgccctgc gcgttggcgc atatcgcctg ccagaaccag atgtcgttat
    34441 cagtttgccc cggtaaacgg ccttcgctta aattctccgg ataatgcagc gcggcatgaa
    34501 gcagttcgct ggcctgctgc ggctgtctgg catcaaggtg ctgccaggcg cgtaataact
    34561 ggttgaggat aaactgactg gtgaccttcc cttccccgcc ttcccacggg tggaatttgc
    34621 gcgtggcgag aatgtccgcc gctttgtctg cctgacccgt gagatgccac aaattgagca
    34681 actctgctgt catgtcgtcg cgtttcagcg caatttccag attattttcc agacgcgcca
    34741 gtcgtttctc cggtgtggct ccgcttaact tatcaagcaa atcccgttcg aaaagcagac
    34801 gtgcatcctg cggcgcaagc tgataagcat tatcaagata acgcgcggcc agctcataat
    34861 cgtgttgctt attccacgca tggatcgcta acccgcgcca gccgtcggca aactccggcg
    34921 acatctctac gcaacgttgc cataaggtaa tggctttgtt gtagctacgc ttgttgtagt
    34981 agaagcaggc cagtaaatga cgggcaaacc agcactcttc aatactctcc agcgccgcca
    35041 cttcttccag cgtattcggg aaacggacaa actgcgggaa gacatcaatg gcttttgcga
    35101 ccagttcgcc acgttcggct ttcggcagca agctggcttg caggtaaagc ggcagcgtgc
    35161 gctggcagtc cagtgcgttc agcatctctg ccgccagggt gggcattccc cagttaatca
    35221 gttgcccggc ggtcatcagg gcgttaacgt cgcgtccctg acacagaccg cgccactgcg
    35281 cgagagctga ttcgctacga ccatcgaacc agttcagcca ccacagagtg gcgttcagcg
    35341 gataatcgcg cagcagtttc tcgcgctgca cacgcgcgtt gtcctgacga ccacttaaca
    35401 ccagcagcag attatgcagg caaagcactt cctgattggt tgggcaggcg cgaagacttt
    35461 gttggcaaaa atccagacca gcgtcgaagt taccattacg cgccgccagt cgtgccaggc
    35521 cataatagcc accggctttg ctgttgccgc tccagaccgc acgccagaaa tcctcttcgg
    35581 cttgttgata ttgtccctga cgttcgtaag cactggcgcg aatcaaactc gcctgtccgc
    35641 actgcggatt tttgttcagc gcatgtgcgc gtttcagagc ctgagtggca tacgccaccg
    35701 cttgcgggaa atctgcgcgg ttatattcca gcatcgccag cgccaggtta cagcgataat
    35761 ccagcgggtc cagcgccacg ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat
    35821 gatattgctc cagatgctga ccgataaacc aggcttcatc tgtactggta atgtcttgtg
    35881 ctgccagtgg cgctttggcg acgtccggca acggcaactc ttgcggctga tgttcctgat
    35941 aactcaggac aatattgccg tcggcatcgg agagctcaat ggtcagcctt tcaggattga
    36001 taccgtgcaa cacgccctgg atggcggtcg caggcatcag tgccacggca tcatcaagta
    36061 acgcgttgca tttgccgatt tcgcggatcg ccaggcggta tccgttcaac ggagagatgg
    36121 catacagccc ccactcaatc ccccgctcac tacgctggag ttttatcacc gcatcgcggg
    36181 aggcattttg caccatgccc aaagaatgat aaggcaggaa atactgctca aaacgcttct
    36241 cttcgtaagc atcaagccag gtaaaatcag gctggttatc ggcaaaaata ccggtcatca
    36301 gttcgatata cgggccgtta ttgtcggtca gactcttgtc ccacgcctgg ccgaattcac
    36361 tgtgtcccca gctccactgt tttttacctg gcgcgatatg gtggttggca acgtgtagca
    36421 aaccgccatc ttcatcgtga caccacgcgc caacaaaatc gtactgtgat ttttcagcca
    36481 tatatgaggt tggaacgggc acatttttat agcgagaaat gtccactccg gcggagtagt
    36541 ccactttgta gtaagtgccg gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat
    36601 caaacaccgc cgttacatcc ggcgggaaga cgctctgatg cccttccccc cctttcactg
    36661 ccgggttggc ccaccacaag aaatgacgcg gcgtggcgtt gccgttatag acgcggctgg
    36721 cgatttccaa cgccgcccgt tcaggacgca gtgtgaaacc tgtcatcacc tgtaaaccgt
    36781 gcatcggctc cgtttcgccg acccacaccg tctgtgcgcc gtcttcatgg gcttcgaggg
    36841 tgaaatcaac gggcataaag gtggtcgggc gatggtgttg cggccagtta aactcaatcc
    36901 cgccagagat ccacggtccc agcagcccca ccagcgcagg tttaatgact tcattgtgat
    36961 agacaaaatc gcgttgtttc actttatccc atgcgcgatg cacacgaccg cccagttccg
    37021 gcaggatcat cactttgatg tagtcgtttt ccagccacac cgcctgccag gatttcaggg
    37081 ttttctgctc gctcagcgta tcggtcacgc cgtagggata aaccgcgccg gacgatccct
    37141 gataaacgcg attttcgagg aacatgggat gtatatcctg cggcccggtt tcataggtcg
    37201 ggatctcaac gcgctcttgc cacactttta ctggagtcat ggtgccctca atattaacaa
    37261 gacatactga attaaaagat ttgtggcagt atattgaaca atatggcaat gttttcgcgg
    37321 aataatcacg caattaacta aacaaggttt agtgaagatg agagcctgca ttaacaatca
    37381 acagattcgc caccataaca aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc
    37441 caataaatca acgctggccc ggctggcgca aatttcgatt ccggcagtca gtaatatttt
    37501 gcaggaactg gaaagcgaaa aacgggtggt gaatatcgac gatgaaagcc agacgcgcgg
    37561 gcatagtagc ggtacatggc tgattgcgca ggaaggtgac tggacgctgt gcctgaacgt
    37621 gacgcccacc agtattgagt gtcaggtggc taatgcttgt ttaagtccga aaggcgagtt
    37681 tgagtattta cagattgatg caccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg
    37741 ctggcatcgc caccgtaaat tgtggccgga ccgcaccatc aatctggcgc tggcaatcca
    37801 cggtcaggtt gatcctgtga ccggcgtgtc gcaaaccatg ccgcaagcgc cgtggacaac
    37861 gccggtggag gtgaaatatc tgctggaaga gaagctcggc attcgggtga tggtcgataa
    37921 tgactgcgtg atgctggcgc tggcggagaa atggcaaaat aattcgcagg aacgggattt
    37981 ctgcgtgatc aacgttgatt acggcattgg ctcgtcgttc gtgattaacg agcaaattta
    38041 tcgcggcagt ttgtatggta gcggacagat tggtcacacc atcgttaatc cggatggcgt
    38101 cgtctgcgac tgtggacgtt atggctgcct ggaaaccgtc gcctcgttaa gcgcattaaa
    38161 aaaacaggcg cgggtatggc taaaatcaca accggttagt actcaacttg atcctgaaaa
    38221 actgactaca gcgcagttaa tcgctgcctg gcaaagtgga gaaccgtgga tcaccagctg
    38281 ggttgaccgc agtgccaatg ccattggttt gagtctgtat aacttcctca acatcctcaa
    38341 tattaatcag atttggttgt acggtcgcag ttgtgccttt ggtgagaact ggcttaatac
    38401 tattattcgc cagacaggat ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc
    38461 gacgcaaatt ggcttcgggc aattaagccg cgcacaacag gtgctgggaa ttggctattt
    38521 gtatgttgag gcgcagttac ggcagatttg atggcgcaat aacgtagaaa ggcttcccga
    38581 aggaagcctt ttttcaacag attaaccgtt tcgcttatgc gtaaaccgga taacgtgcgc
    38641 agatgtcgag aactttacct ttgatgcgct cgataacagc ttcatcattg atgctgtcca
    38701 gcacgtcaca catccagcca gccagttctt tcgcttctgc ttctttgaag ccgcgacgcg
    38761 taattgccgg agtacccacg cgaataccgg aggtcacaaa cgggctcttc ggatcgttcg
    38821 gtacgctgtt tttgttgacg gtgatgttag cacggcccag agcggcgtct gcttctttac
    38881 cggtcaggtt tttatcaacc agatcaacca ggaacaggtg gttatcagtg ccgccggaaa
    38941 ccactttgta gccgcgttcc aggaacactt ctaccatcgc tttagcgttt ttagcgacct
    39001 gctgctggta agttttgaac tcaggctcca tcgcttcttt cagagcaacc gctttaccag
    39061 cgattacatg catcaacgga ccgccctgac caccagggaa aacggcagag ttcagttttt
    39121 tgtacagctc ttcgctacca cctttcgcca ggatcagacc gccgcgcgga cccgccaggg
    39181 ttttgtgagt ggtggtagta acaacgtgag catgaggaac cgggttcggg tagacgccag
    39241 cagcaaccag gcccgcaacg tgcgccatat caacgaacag gtaagcaccg atgctgtcag
    39301 cgatttcacg cattttcgcc cagtccacca cgccggaata tgcagagaaa ccaccgataa
    39361 tcattttcgg cttgtgttct ttggcttgtt tttccagatc ggcgtagtcg atatgaccgg
    39421 tagcatcgat accgtaagga acgatgttgt acagtttacc ggagaagtta accggagaac
    39481 cgtgagtcag gtgaccgcca tgcgccaggt tcatacccag aacggtatca cctggttcca
    39541 gcagcgcggt gtagaccgca aagttagcct gggagccgga gtgcggctgg acgttagcgt
    39601 agtcagcgcc gaacagttct ttcgcacgat cgatcgccag ttgttcaacg atatcaacat
    39661 actcgcaacc gccgtagtag cgtttgcccg gataaccttc agcatatttg ttggtcagct
    39721 gagaaccctg cgcctgcatt acgcgcgggc tggtgtagtt ttcggaggcg atcagttcga
    39781 tgtgctcttc ctgacgtact ttttcctgct ccatagcctg ccacagttcg gcatcataat
    39841 cggcaatgtt catttcacgc tttaacatcc gcatctcctg actcagctaa caataaaatt
    39901 tttggccttt ataggcggtc ctgttggaca acggcgaaca gtataaccga atcattgtgc
    39961 gataacaggt cttgacaaag gaatttacgc aaacgattac cttcaggcta cgcaaggctt
    40021 tggagaataa agagcttgca accggaaacg gatttctttt caggtttgtg atgcaaattt
    40081 ttcacttcat cacattcttt ctgaaaaaca ccaaagaacc atttacattg cagggctatt
    40141 ttttataaga tgcatttgag atacatcaat taagatgcaa aaaaaaggaa gaccatatgc
    40201 ttgacgctca aaccatcgct acagtaaaag ccaccatccc tttactggtg gaaacggggc
    40261 caaagttaac cgcccatttc tacgaccgta tgtttactca taacccagaa ctcaaagaaa
    40321 tttttaacat gagtaaccag cgtaatggcg atcaacgtga agccctgttt aacgctattg
    40381 ccgcctacgc cagtaatatt gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg
    40441 cgcagaagca caccagcttc cagatcaaac cggaacagta caacatcgtc ggtgaacacc
    40501 tgttggcaac gctggacgaa atgttcagcc cggggcagga agtgctggac gcgtggggta
    40561 aagcctatgg tgtactggct aatgtattta tcaatcgcga ggcggaaatc tataacgaaa
    40621 acgccagcaa agccggtggt tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac
    40681 cgcgcagcgc gcttatcacc agcttcgaac tggagccggt cgacggtggc gcagtggcag
    40741 aataccgtcc ggggcaatat ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg
    40801 aaattcgtca gtactctttg actcgcaaac cggatggcaa aggctatcgt attgcggtga
    40861 aacgcgaaga gggtgggcag gtatccaact ggttgcacaa tcacgccaat gttggcgatg
    40921 tcgtgaaact ggtcgctccg gcaggtgatt tctttatggc tgtcgcagat gacacaccag
    40981 tgacgttaat ctctgccggt gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg
    41041 caaaagcagg ccacacagca caagtgaact ggttccatgc ggcagaaaat ggcgatgttc
    41101 acgcctttgc tgatgaagtt aaggaactgg ggcagtcact gccgcgcttt accgcgcaca
    41161 cctggtatcg tcagccgagc gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc
    41221 tgatggattt gagcaaactg gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct
    41281 gcggcccggt tggcttcatg cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc
    41341 aggaaaacat tcattacgaa tgctttggcc cgcataaggt gctgtaattt gatgttgccg
    41401 gatggaaaca tccggcaacc cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg
    41461 tacggatgcg aatgacccgt gccacgtcaa agacgaagat tttaccgtca ccgattttgc
    41521 cggtttgcgc cgtgcgaata atggtatcga cacaggtatc gacaatgtcg tccggtacga
    41581 caatctcaat tttcactttc ggcagaaaat ccaccatata ctccgcgccg cggtacagct
    41641 cggtatggcc tttctggcga ccaaagcctt tcacttcggt caccgtcatg ccggtaatac
    41701 cgacttcggc cagtgcttcg cggacatcgt ccagcttgaa gggttttata atcgcatcaa
    41761 tctttttcat gctattcctt gaaaaggtcg cctgtctttt gatctgctaa acgtaacaca
    41821 taacgccaat tcattccttg aaatcgtttg catccagctc gtgtcgggaa agcagtttat
    41881 aaaattctgt ccggttgcgc cccgccattc tcgccgcgtg ggtgacgttg cctttggtga
    41941 tttgcagcag cttacgcaaa tagttgagtt caaactgatt acgtgcctca acaaaggttg
    42001 gcagcgccgt gttttcaccc tccagcgcct gctccaccag cgcatcacta atcaccggag
    42061 atgaggtcag cgccacgcac tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc
    42121 agctcgcggt catcaggcgt ttcatcgcat cggtagagaa cgcgcggaca aacggtttat
    42181 gtcgctctgc cgcctggcgc aacaggtgat ttgccagtag cggaatgtct tctgtgcgct
    42241 ccgccagtgc cggaattttc aggctgacaa cgttgaggcg gtaatagaga tcttcgcgga
    42301 attccccgcg cgccatcgct tttggcaggt cgcggtgagt ggcggaaata atccgcacgt
    42361 cgatatcaat atcgcggtta ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca
    42421 gcagtttgac ctgcaacggt gcgggcatat cgccaatctc atcgagaaat agcgtaccgc
    42481 cttccgccgc ctggaataaa ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg
    42541 catgaccaaa cagctccgac tccagcaatt gctcaggcaa tgcaccacag ttaatagcaa
    42601 taaatggttt gctattgcgc gggctggcgt tgtggatagc ctgggcgaaa atctctttcc
    42661 cagtgccgct ctggccatta atcaaaacgc tgacgtctga ttgcgccacc agccgcgcct
    42721 gttcaagcaa acgcagcatc agcgggctgc gggtgactat cgcctcgcgc cagcgttcgt
    42781 cggtggctgg cgcggattgt tccagcgcat catcaattgc ctgatatagc gcatctttat
    42841 cgacaggctt ggtgaggaaa ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggga
    42901 tagaaccatg cgcagtaaga ataattactg gcattccagg ctgcactttc tggatttcag
    42961 caaacagctg cataccgtcc atttcatcca tccgcaggtc gctgataact aaatctactt
    43021 tttcgcgatt cagtacccgt aatccttcag caccactctc cgccgtgacc acactgtagc
    43081 cttcgctggt caggcgcagg ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata
    43141 ataaatgcgc aggtttatgg ctcatcagga gtgacctcat gggtggatgg cgcgggcttt
    43201 tcactctcgt gcggcgtatc cggcgagaaa tttccagccg gtttgcgggt cgagagctgg
    43261 cgttcaatat cggtcaggtt ttccagcttg cgggtggtga gttccagttg ctgctgtaga
    43321 acgtgatgtt gctggcgcaa tgtatccagc tcgctgtcgc tggactgctg gagtttactg
    43381 taacgttggc gctcttccgc cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga
    43441 tacagtgggc gaacctgtgc tgggatctcg gtacttaacg cttcaatacg cgcgaccagc
    43501 tggcggcgct catacggcgt aattttggcg tcggcgagca aaatcccttg tttaaaggta
    43561 ttttgccagc tgccgtcgtc atattggcga gcttgctgac gcgactgcgc aggcattaaa
    43621 cgatcagcac aatccatcgc ccgcagccag taaagcggat tggtttcggt tgatttacct
    43681 tgcagcgccc agatgtcgct acattcagta gaaagatagt cagccagttg ataaaccgga
    43741 attttttctt ctgctggcgt atcaatggct ggcttattgt gattctgcac gcaacccagc
    43801 aatgccagac atggcagccc tgccagccac agccgtcggg gcaataatcg ttgaaaaatg
    43861 tgtcgcatat tcaccagact taaagcctat ctcagtgggc gtaattgttg cagacagtct
    43921 ggacatggac agcgcggaga aaccggagcg tacatatcgt acgtgaagat ttcgagcact
    43981 gcccggggcc gaactgacaa ataaaatagc ctgataggat aggctgttag cattatttcg
    44041 tgtttttcga cgacggtaat tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca
    44101 gatacagttc cccttgcata cggcgaatac aatccctggc aatgcttaat cccagaccgc
    44161 tgcccttcac cgcccctttt cgctggtggc ttccctgaaa aaagggttcg aagatcatgg
    44221 cgcgttcctc ttgcggaatg ggcgtgcctg tattgatgac atcaatataa acccgcgcac
    44281 catgtgaact gctgcgaagg caaatgttac cggattcagc cccgtagtgc accgcattgg
    44341 agtaaagatt atccagtacg ctcatcagca gcattggctc cgccaggcaa gctgttgctt
    44401 tgagatcgac gtcggtatgc atcattttag cccgtgcggg caggctatga gcagaaacca
    44461 ctgtctccac cagcggtgct aattcaacat tctccagttc caccgcactg tccgcctgtt
    44521 tacggttgta atcaagcagt tgttcgatca gtttttgcaa attgcggctg ctgctatcaa
    44581 gaatgctcac cacctctttt tgctctggct taagcggccc gacaacctgg tcagccagta
    44641 attcagtgcc ctcgcgcata ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta
    44701 aaaattgatg gcgttgggat tccagccatg acaggcgctc acttaaccaa agaatacgtt
    44761 gcccaaccga gcgtaactcg ctcggtccac tgaacgaaac gctattgccc agagaacgcc
    44821 cttcccccag acggttgatc atgcgctcga tatttttcac cggcccgata atcatccgcg
    44881 tgaaaagtag caccatcacc agactcacca gaaatagcac cagcgattgc caaccaaaat
    44941 attgcccacg ttcggcgatt tcacgctgaa gttgctgccc acgagagaac accactgtgc
    45001 gcgtggcctg caccatttcg gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg
    45061 cagcatcggg accactgttg ttacactgaa gttgggccag attgttcaaa tcctgacgca
    45121 atgcctggta aagtttatcg tctggcagta tgcctgcgtg ggcatcgagc atttcgctgt
    45181 aacgcttgcg ctggctttga taaaccttcg caagcgtagg gtcgtccagc acgcaatact
    45241 gacggtagct acgctccatc tccagcgccg cgttggtcat cgcttcacta cgccgggcat
    45301 caataagcgt agtgcggtta accagcgccg cctgatcgct aagcgcattc aggctttgcc
    45361 aggcttgcca tgccagcacc aacaggggta gtagaattag caaaaatgcc agcattacca
    45421 gttgtcgtaa tgagcgggga aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg
    45481 cgaatagcct gatgctaacc gaggggaagt tcagatacaa caaagccggg aattacccgg
    45541 ctttgttatg gaataaggcg gtgcctaact cgacgtttcg cccgatggtt gatatagcta
    45601 cgctgatatc agaagttgga cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag
    45661 cacgtcccga aggggctgac ataagtcggt gaatgagcca ctgcttacta ttatgcagta
    45721 actgtgccaa taaagaaaat agtttggtaa cgtactgatt atacgttgct ttgaggggtt
    45781 tatgtcttct ccgctgtttg aatgtaaatc agccactgtg tcgctaaaaa gagacaactt
    45841 aagataaact tattagataa tattaaaatc aatgagttaa gtgtcgccag aaagcgacac
    45901 ggcaaaccac ccattgtcgt gatttacaga cacaaaaaag ctcccggagt tgggagctta
    45961 tgatagtggt tggtgcttaa tgccgcatcc gggctgcaaa accaatgggc tgacgactta
    46021 ccccaactgc ttacgcgcat tgcggaaaat acgcatccat gggccatcct cgccccagtt
    46081 ttccggatgc caggagttgc tgacagtacg gaaaacacgt tccgggtgcg gcatcataat
    46141 ggtgactcga ccactttcag tcgtgactgc cgtaataccg ttcggtgaac cgttcgggtt
    46201 agccgggtag gtttcagtga ctttgccgaa gttatcgaca tagcgcagtg ccaccagccc
    46261 tttgctttcc agtgccgcca gatgcgccgc atcacgcact tccacgcgcc cttcaccatg
    46321 agagacagca atcggcattt gcgagcccac catcccctgc aacagcagcg acgggctttg
    46381 ggtcacttca accaggctga aacgcgcttc aaagcgatcg gaggtattgc gcacaaaacg
    46441 tggccacaac tcactacctg ggatcagttc gcgcaggtta gacatcatct ggcaaccgtt
    46501 acataccccc agcgccagcg tttgcggacg gtggaagaag gttgcaaact catcgcgtac
    46561 acggtcattg aacaggattg acttcgccca accttcaccg gcacccagca catcaccgta
    46621 ggagaaacca ccgcacgcga ccagggcgtg gaaatcttcc aggcccgtgc gtccggtcag
    46681 caggtcactc atatgcacgt cgatagcatc aaagcctgca cggtggaaag ctgccgccat
    46741 ttcaacatgc gagttcacgc cctgctcacg cagtacggca actttcggac gtgcgccagt
    46801 ggcaatatac ggtgctgcca catcttcgtt gatatcgaac gacagtttta cattcaggcc
    46861 aggatcggca tcgttagatt tcgcctggtg ctcctggtca gcacactccg ggttgtcacg
    46921 caggcgctgc atctgccagg tagtttctgc ccaccagaca cgcaacgtgg tgcggctttc
    46981 gctgaataca gtctgcccgt tggcggtaat cacaaaacgg tcaccggaaa ccgcctgccc
    47041 tacataatgg acacaatcag caagcccatg ctgtgccagt acggactcaa ccgcttcacg
    47101 gtcagcggca cgaacctgaa tcaccgcacc cagttcttcg ttaaacaacg ccgccaggcg
    47161 atcgtcgccc agagaggcaa tatccgcgtt aatgccgcaa tgaccagcaa aggccatttc
    47221 cgccagcgtt accagcaggc cgccatcaga gcggtcgtgg tatgcgagca gcttacgctg
    47281 tgcaaccagc gcctgaatcg cgtcatagaa gcctttcagt tgcgcgacgt cgcgtacatc
    47341 tgccggtttg tcgccaagct gacgataaac ctgcgccagc gccgttgcgc ccagcgcgtt
    47401 attgcctttg cccaaatcaa tcagcagcag tgcgttatct tcggtagaaa gctgtggcgt
    47461 gatggtgtga cgcacatctt ccacgcgggc aaatgcagaa atcaccagcg acagcggcga
    47521 cgtcatttcg cgctcttcgt taccttcctg ccagcgggtt ttcatcgaca tagagtcttt
    47581 acccaccggg atcgtcaggc ccagcgccgg acaaagctct tcgcccacgg ctttaacggc
    47641 ttcatacagg cccgcatctt caccagggtg gcctgccgcc gccatccagt tggcggaaag
    47701 tttaatgcgt ttgatatcac caatctgtgt tgcggcaata ttggttaacg cttcaccgac
    47761 cgccagacgg gcagaggcgg cgaaatccag cagcgcaacc ggcgcacgct cgccaatcgc
    47821 catcgcttca ccgtagtagc tgtcgaggct ggcggtagtg accgcgcagt tagcgaccgg
    47881 cacctgccac ggccccacca tctgatcgcg cgctaccatg ccggttacgc tgcggtcgcc
    47941 aatggtcacc aggaaggttt tttccgccac agtcggcaga tgcagcacac gtttcaccgc
    48001 atctgcaatg gtgatccctt cacgggccag cgcgtcgcct ttcgctttca gcgtttgtac
    48061 atcgcgggtc atcttcggcg ttttaccaag caggacgtcc agcggcagat cgatcggctg
    48121 attatcaaaa tgacgatcgt gcagagaaag atgcagttct tcggtcgctt caccaatcac
    48181 cgcgtagggt gcgcgttcac gcttacacag ttcgtcaaac agcggtaatt gatcggcagc
    48241 aatcgccagc acgtagcgtt cctgggattc gttacaccag atttccagcg ggctcatgcc
    48301 cggttcgtcg cttagaatat cgcgcagttc aaatttaccg ccgcgtccgc cgtcgctcac
    48361 cagctccggc atcgcgttag aaagaccgcc cgcaccgacg tcgtggataa acagaattgg
    48421 gttagcatca ccaagctgcc agcaacggtc gatcacttcc tggcaacgac gctccatctc
    48481 cgggttgtcg cgctgtacgg aagcaaagtc gaggtcggca tcagactgac ctgacgccat
    48541 ggaagacgcc gctccgccgc caagaccgat gttcattgcc gggccgccga gaacgaccag
    48601 cttcgcaccg acgttgatct cgcctttttg tacgtgatcg gcgcgaatgt tgccgatccc
    48661 gcccgccagc atgatcggtt tgtgataacc gcgcagctct tcgccgttgt ggctgttcac
    48721 tttttcttca taagtacgga agtagccatt cagtgccgga cgaccaaatt cgttgttaaa
    48781 cgccgcgccg cccagcgggc cttcggtcat gatgtccagc gcggtgacaa tgcgctcagg
    48841 cttaccgaaa tcttcttccc acggctgttc gaagccagga attcgcaggt tggaaacgga
    48901 gaaaccaacc aaacccgctt tcggcttcgc gccgcgcccg gtggcacctt catcgcggat
    48961 ttcaccgccg gaaccggtcg ccgcgcccgg ccacggagaa atcgccgtcg ggtggttgtg
    49021 agtttcgact ttcatcagaa tatgcgccgg ttcctgatgg aaatcgtagc ggcccgtttc
    49081 gtggtcagca aagtagcggc ccacttcaga accttccatt acggcggcgt tatctttata
    49141 agcagagaga acgtgatctg gcgtggtttc gaaagtattt ttgatcatcc tgaacagcga
    49201 tttcggctgt tgttcaccgt cgataatcca gtcggcgtta aaaattttgt ggcggcagtg
    49261 ctcagagttc gcctgagcga acatatacag ctcgatgtcg ttcgggttac gaccaagctt
    49321 tgtgaaagca tcctgcagat agtcaatttc atcttccgcc agagccaggc caagacgcag
    49381 gttagcgtcg atcagcgcct gacggccctg ccccagcaaa tcaacgcttg tgaccggagt
    49441 cggttgatgg tgggcaaaca actgctctgc atcatctaaa tcaaaaaaga ccgtttccat
    49501 catgcggtcg tgcagttcag cggtaacctg ctgccattgt tcattggtca gcgtaccggc
    49561 ttctatatag taagcaacgc cgcgctcaag gcggtttacc tgttgtagcc cgcagttatg
    49621 ggcaatatcg gtcgctttcg aagaccaggg agagatggtg ccaggacgcg gggtcaccag
    49681 caggagtttg ccttgcgggg cgtggctggc gagtgccgga ccatatttca gcaggcgttc
    49741 aagttgtgcg tgctcatcat cgtttaacgg cgcattgagg tcagcaaaat ggacatactc
    49801 ggcgtaaata ttgtgaaccg ggagcctggc agcctgaaaa cgtgccagca gtttgttgat
    49861 tcggaatgcc gacagtgcag gcgaaccacg cagaatttcc atcataagtc tctcgtcttc
    49921 taagctttcg gtgtacccaa gggggggaaa cgggcgtcat tataaagaat ctgatgcgct
    49981 gacgaaaccg tttgcgtgga aataaaatca ccatcgtgaa ttagcaacgc gtgccgccaa
    50041 tggatgtaat aagttgctat ctggcgcagg tttacgcaaa atgccgctca tttatgagta
    50101 aacctttcac tattattacg ttttttcaag ctgggacgcg cacgacacag agaattaact
    50161 aattgaaaaa attaaagatt aattatctgt tcatcggcat tctggcactg ctgctcgcgg
    50221 tcgctctctg gccatccatt ccctggtttg gtaaagccga caaccgtatc gccgccattc
    50281 aagcgcgggg agagttgcgt gtgagcacca ttcatactcc cctgacttat aacgaaatca
    50341 acgggaaacc ttttggcctg gattacgaac tggcgaaaca gtttgccgat tacctcggcg
    50401 taaaactgaa agtgaccgtg cggcagaata tcagccagct gtttgacgac cttgataatg
    50461 gtaacgccga cctgctggcg gcaggacttg tctataacag tgagcgggta aaaaattatc
    50521 agcctggccc tacctattat tccgtgtcac aacaactggt ttataaagtg ggtcagtatc
    50581 gcccacgtac gctgggcaac ctgacggcgg agcaactcac cgttgcaccg ggtcatgtgg
    50641 tggttaacga tctccagacc ctgaaagaaa caaaattccc ggaattaagc tggaaggtag
    50701 acgacaaaaa aggctctgcg gaattaatgg aagatgtcat cgaaggaaaa ctcgattaca
    50761 ccattgctga ttctgtcgcc atcagcctgt ttcagcgcgt tcacccggag ctcgccgtag
    50821 cgctcgatat caccgatgaa caaccggtga cttggtttag cccgttagat ggcgataata
    50881 ccctttccgc cgccctgctc gacttcttca acgaaatgaa tgaagacggt acgctggcac
    50941 gcattgaaga gaaatacctg gggcatggcg atgattttga ttacgtcgat acgcgcacat
    51001 ttttacgcgc cgtcgatgcg gtactgccgc agttaaagcc cctgtttgag aaatacgccg
    51061 aagaaattga ctggcgtttg ctggccgcta ttgcttatca ggaatcgcac tgggatgcac
    51121 aggccacttc accgacgggt gtgcgcggca tgatgatgtt aaccaaaaat accgcgcaaa
    51181 gcctcggcat tacggatcgt accgatgccg aacagagcat cagcggtggc gtgcgttatt
    51241 tgcaggatat gatgagtaaa gtgccggaaa gtgtgccgga gaacgagcgg atctggtttg
    51301 ccctcgctgc gtacaatatg ggctatgcgc atatgctgga tgcccgcgcc ctgacggcaa
    51361 aaaccaaagg gaatcctgac agttgggctg acgtaaaaca gcgtctgcct ttacttagcc
    51421 agaaacccta ttacagcaag ctgacttacg gctacgctcg tggacatgaa gcctacgctt
    51481 atgtcgaaaa tattcgtaag tatcagatta gcctggtggg ttatctgcaa gagaaagaga
    51541 agcaggctac agaagcggcg atgcaactgg cgcaggatta tccggcggta tcgcctacgg
    51601 agttgggcaa agagaaattt ccttttctct cgtttctttc ccagtcgtca tcaaactatt
    51661 tgacccattc tccctctctg ctgttttcca ggaaagggag tgaagagaaa caaaattaat
    51721 ccgtcgagga ttgcgctttt ttctgcgctt taatttcctg gcggcgcatg cgaaagaagt
    51781 cactgagcaa cgccgcgcac tcatccgcca gtattccttc cgtaatttcc actcggtgat
    51841 tcatacccgg atgatgcagc acatccatta aagatcccgc agcgccagtt ttcgcgtcac
    51901 gcgcaccaaa gaccacgcga ccaatgcgac tgtggatcat cgctccggca cacattacac
    51961 atggttcaag cgtgacatac aacgtggcgt cgatcagacg ataattttgc atcaccagac
    52021 caccctgccg cagggccatg atttctgcat gtgcggtggg atcatggcga ccaatcgggc
    52081 ggttccagcc ttcgccgatt acccgattgt tatgcactaa taccgcgccg accggcactt
    52141 cccgctcatc ccaggcacgt ttcgccagcg tcagcgcgtg acgcatccag tattcgtggc
    52201 taaattcgac ttcagacaaa aagaaaactc cggttataaa agcgcggcgc attatacacg
    52261 gacgctatgc tttactctat tccagttgct ggagttcacc gcgcggggtt actcgccaac
    52321 gatgctgaca gaaataaagc agcggattgt cctgattact gtcgctatag ccactgtaca
    52381 gccgcagcgg agtgccgatt ttgcgctcca gttgtgcgac cttttcatgt cccagacaac
    52441 gcatcgtcaa tacccaacca ccatagccac gctgaatttg gctggcgata agattaaccc
    52501 gcggcagcca gggcgtatcg aaataaaccg cttcaaccag cggctgcgga gagccggtaa
    52561 tcaaccagat atcagcatcg gaacttaaca ggtaggtggt taatcgctcc tgaaccagcg
    52621 gaaaggcggt aacattgtcg cgaaaccagc gcacgaaatc ggcctgcaac gtctgtaaac
    52681 gtgcttcgct gtgaccaaaa gtgcaccccc acagaagcag actcatcggc cagcgtgccg
    52741 cacgaccttt tatcaataac gcaatggcta taatcggtaa caacggcagg acaagtaacg
    52801 cattcagcgg ttggcgacgc agtaaatagc gcagaaaact gccgaacata tcctgctgat
    52861 gcaatgttcc atctaagtca aaaaacacca cacgacgctc gtgagttgcc aaaccatact
    52921 cctctggact gaaacatcct gattaatttt cgctactaca tagcctaaca gatagatcat
    52981 cacttttccg gcaacaatcg accgcatcat ggctaactgg aatttttaat tcatggcaat
    53041 tagcggcaat ggaatataaa attcactcgc gtgtctctca tattgcctgt tgtcgccact
    53101 atttcatcgc gaggaaagat atgaacggct tacttcgtat ccgtcagcgt taccaggggc
    53161 ttgcccagag tgataaaaaa ctggcggatt atctgctgct acaacctgat acggcgcgcc
    53221 atttaagctc tcagcaactg gccaacgaag ccggagtcag tcagtccagc gtcgtgaagt
    53281 tcgcgcaaaa actcggctat aaaggttttc cggcgcttaa gttggcgttg agtgaagcgc
    53341 tggcaagcca gccggaatcg ccctccgtgc ccattcataa ccaaatccgc ggtgatgatc
    53401 cgttacggct ggtcggcgaa aaactgatta aagaaaatac tgccgctatg tacgcaacgc
    53461 taaacgttaa tagtgaagag aaactgcatg aatgcgtaac aatgttgcgc tctgcgcggc
    53521 ggataattct gaccggtatt ggcgcttcgg gtctggtggc gcaaaacttt gcctggaagc
    53581 tgatgaagat tggcttcaat gctgccgcag tgcgcgatat gcatgcgcta ctcgcaacag
    53641 tacaggcgtc gtcccctgac gatctgttat tagccatttc ctacaccggt gtacgacgcg
    53701 agttaaacct ggcggcagat gagatgctgc gagtgggcgg aaaagtgctg gcgattaccg
    53761 gctttactcc gaatgccctg caacagcgtg cttctcattg cctgtatacc attgccgaag
    53821 aacaagcgac aaacagcgct tcaatctctg cttgtcacgc tcagggaatg ttaacggatt
    53881 tgctgttcat tgcgctgatt cagcaggatc tggaactggc accagaacgt attcgtcata
    53941 gtgaagcgct ggtgaaaaaa ctggtctgag taaagaatgc gcgtataatg cccgcccggt
    54001 ttgtgttgtt ttgagagttt ccttatggcg ttgttaatca ctaaaaaatg catcaattgt
    54061 gatatgtgtg aacccgaatg cccgaatgag gcgatttcaa tgggagatca tatctacgag
    54121 attaacagcg ataagtgtac cgaatgcgta gggcactacg agacaccaac ctgccagaag
    54181 gtgtgcccga tccccaatac tattgtgaaa gatccggcgc atgtcgagac agaagaacag
    54241 ttgtgggata aatttgtgct gatgcaccac gcggataaaa tttaatattc tactctggaa
    54301 gtagagtatt aattatatta ctgggaagcc ttaacgcca
//
LOCUS       DAFNBJ010000011        50377 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000011 DAFNBJ010000000
VERSION     DAFNBJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.011"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            209..1156
                     /gene="cyoA"
                     /locus_tag="L2X49_000665"
     CDS             209..1156
                     /gene="cyoA"
                     /locus_tag="L2X49_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HBN4795806.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1178..3169
                     /gene="cyoB"
                     /locus_tag="L2X49_000666"
     CDS             1178..3169
                     /gene="cyoB"
                     /locus_tag="L2X49_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HBN4795807.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3159..3773
                     /locus_tag="L2X49_000667"
     CDS             3159..3773
                     /locus_tag="L2X49_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBN4795808.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3773..4102
                     /locus_tag="L2X49_000668"
     CDS             3773..4102
                     /locus_tag="L2X49_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBN4795809.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4114..5004
                     /gene="cyoE"
                     /locus_tag="L2X49_000669"
     CDS             4114..5004
                     /gene="cyoE"
                     /locus_tag="L2X49_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBN4795810.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5153..6517
                     /locus_tag="L2X49_000670"
     CDS             5153..6517
                     /locus_tag="L2X49_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4795811.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(6645..7136)
                     /gene="yajQ"
                     /locus_tag="L2X49_000671"
     CDS             complement(6645..7136)
                     /gene="yajQ"
                     /locus_tag="L2X49_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBN4795812.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7304..8215
                     /gene="panE"
                     /locus_tag="L2X49_000672"
     CDS             7304..8215
                     /gene="panE"
                     /locus_tag="L2X49_000672"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBN4795813.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVATPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8178..8768
                     /gene="yajL"
                     /locus_tag="L2X49_000673"
     CDS             8178..8768
                     /gene="yajL"
                     /locus_tag="L2X49_000673"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBN4795814.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8822..10270)
                     /gene="thiI"
                     /locus_tag="L2X49_000674"
     CDS             complement(8822..10270)
                     /gene="thiI"
                     /locus_tag="L2X49_000674"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBN4795815.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10476..10718
                     /gene="xseB"
                     /locus_tag="L2X49_000675"
     CDS             10476..10718
                     /gene="xseB"
                     /locus_tag="L2X49_000675"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBN4795816.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10718..11617
                     /gene="ispA"
                     /locus_tag="L2X49_000676"
     CDS             10718..11617
                     /gene="ispA"
                     /locus_tag="L2X49_000676"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBN4795817.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            11642..13504
                     /gene="dxs"
                     /locus_tag="L2X49_000677"
     CDS             11642..13504
                     /gene="dxs"
                     /locus_tag="L2X49_000677"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBN4795818.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            13684..14658
                     /gene="yajO"
                     /locus_tag="L2X49_000678"
     CDS             13684..14658
                     /gene="yajO"
                     /locus_tag="L2X49_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBN4795819.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSFSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(14712..15230)
                     /gene="pgpA"
                     /locus_tag="L2X49_000679"
     CDS             complement(14712..15230)
                     /gene="pgpA"
                     /locus_tag="L2X49_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBN4795820.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15208..16185)
                     /gene="thiL"
                     /locus_tag="L2X49_000680"
     CDS             complement(15208..16185)
                     /gene="thiL"
                     /locus_tag="L2X49_000680"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBN4795821.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(16263..16682)
                     /gene="nusB"
                     /locus_tag="L2X49_000681"
     CDS             complement(16263..16682)
                     /gene="nusB"
                     /locus_tag="L2X49_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBN4795822.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(16702..17172)
                     /gene="ribE"
                     /locus_tag="L2X49_000682"
     CDS             complement(16702..17172)
                     /gene="ribE"
                     /locus_tag="L2X49_000682"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBN4795823.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17261..18364)
                     /gene="ribD"
                     /locus_tag="L2X49_000683"
     CDS             complement(17261..18364)
                     /gene="ribD"
                     /locus_tag="L2X49_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBN4795824.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(18368..18817)
                     /gene="nrdR"
                     /locus_tag="L2X49_000684"
     CDS             complement(18368..18817)
                     /gene="nrdR"
                     /locus_tag="L2X49_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBN4795825.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            18968..19507
                     /locus_tag="L2X49_000685"
     CDS             18968..19507
                     /locus_tag="L2X49_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBN4795826.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            19806..20690
                     /gene="tsx"
                     /locus_tag="L2X49_000686"
     CDS             19806..20690
                     /gene="tsx"
                     /locus_tag="L2X49_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBN4795827.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(20867..21214)
                     /locus_tag="L2X49_000687"
     CDS             complement(20867..21214)
                     /locus_tag="L2X49_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBN4795828.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21343..22314)
                     /gene="secF"
                     /locus_tag="L2X49_000688"
     CDS             complement(21343..22314)
                     /gene="secF"
                     /locus_tag="L2X49_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBN4795829.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22325..24172)
                     /gene="secD"
                     /locus_tag="L2X49_000689"
     CDS             complement(22325..24172)
                     /gene="secD"
                     /locus_tag="L2X49_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBN4795830.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(24200..24532)
                     /gene="yajC"
                     /locus_tag="L2X49_000690"
     CDS             complement(24200..24532)
                     /gene="yajC"
                     /locus_tag="L2X49_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBN4795831.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24555..25682)
                     /gene="tgt"
                     /locus_tag="L2X49_000691"
     CDS             complement(24555..25682)
                     /gene="tgt"
                     /locus_tag="L2X49_000691"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBN4795832.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(25738..26808)
                     /gene="queA"
                     /locus_tag="L2X49_000692"
     CDS             complement(25738..26808)
                     /gene="queA"
                     /locus_tag="L2X49_000692"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBN4795833.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            26901..27482
                     /gene="acpH"
                     /locus_tag="L2X49_000693"
     CDS             26901..27482
                     /gene="acpH"
                     /locus_tag="L2X49_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HBN4795834.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(27487..29304)
                     /gene="malZ"
                     /locus_tag="L2X49_000694"
     CDS             complement(27487..29304)
                     /gene="malZ"
                     /locus_tag="L2X49_000694"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBN4795835.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29460..30833)
                     /gene="proY"
                     /locus_tag="L2X49_000695"
     CDS             complement(29460..30833)
                     /gene="proY"
                     /locus_tag="L2X49_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBN4795836.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFEH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(30909..32228)
                     /gene="brnQ"
                     /locus_tag="L2X49_000696"
     CDS             complement(30909..32228)
                     /gene="brnQ"
                     /locus_tag="L2X49_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBN4795837.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(32635..33930)
                     /gene="phoR"
                     /locus_tag="L2X49_000697"
     CDS             complement(32635..33930)
                     /gene="phoR"
                     /locus_tag="L2X49_000697"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBN4795838.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(33988..34677)
                     /gene="phoB"
                     /locus_tag="L2X49_000698"
     CDS             complement(33988..34677)
                     /gene="phoB"
                     /locus_tag="L2X49_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBN4795839.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            34867..36069
                     /gene="sbcD"
                     /locus_tag="L2X49_000699"
     CDS             34867..36069
                     /gene="sbcD"
                     /locus_tag="L2X49_000699"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBN4795840.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            36066..39209
                     /gene="sbcC"
                     /locus_tag="L2X49_000700"
     CDS             36066..39209
                     /gene="sbcC"
                     /locus_tag="L2X49_000700"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBN4795841.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            39335..40519
                     /gene="araJ"
                     /locus_tag="L2X49_000701"
     CDS             39335..40519
                     /gene="araJ"
                     /locus_tag="L2X49_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBN4795842.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYMMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(40662..41570)
                     /gene="mak"
                     /locus_tag="L2X49_000702"
     CDS             complement(40662..41570)
                     /gene="mak"
                     /locus_tag="L2X49_000702"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBN4795843.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            41695..42606
                     /gene="rdgC"
                     /locus_tag="L2X49_000703"
     CDS             41695..42606
                     /gene="rdgC"
                     /locus_tag="L2X49_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBN4795844.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(42684..42767)
                     /locus_tag="L2X49_000704"
     CDS             complement(42684..42767)
                     /locus_tag="L2X49_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795845.1"
                     /translation="MTQRPWSKLQRKKHNIAALKIIARRSE"
     gene            complement(42764..43099)
                     /locus_tag="L2X49_000705"
                     /pseudo
     CDS             complement(42764..43099)
                     /locus_tag="L2X49_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(43254..43538)
                     /gene="ppnP"
                     /locus_tag="L2X49_000706"
     CDS             complement(43254..43538)
                     /gene="ppnP"
                     /locus_tag="L2X49_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBN4795846.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(43610..44287)
                     /locus_tag="L2X49_000707"
     CDS             complement(43610..44287)
                     /locus_tag="L2X49_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBN4795847.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNRGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(44545..44736)
                     /gene="yaiA"
                     /locus_tag="L2X49_000708"
     CDS             complement(44545..44736)
                     /gene="yaiA"
                     /locus_tag="L2X49_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBN4795848.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(44786..45310)
                     /gene="aroL"
                     /locus_tag="L2X49_000709"
     CDS             complement(44786..45310)
                     /gene="aroL"
                     /locus_tag="L2X49_000709"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBN4795849.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(45487..45951)
                     /locus_tag="L2X49_000710"
     CDS             complement(45487..45951)
                     /locus_tag="L2X49_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBN4795850.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAAINPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRGQM"
     gene            46071..46880
                     /gene="proC"
                     /locus_tag="L2X49_000711"
     CDS             46071..46880
                     /gene="proC"
                     /locus_tag="L2X49_000711"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBN4795851.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(46897..48012)
                     /gene="adrA"
                     /locus_tag="L2X49_000712"
                     /gene_synonym="dgcC"
     CDS             complement(46897..48012)
                     /gene="adrA"
                     /locus_tag="L2X49_000712"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBN4795852.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(48114..48434)
                     /gene="psiF"
                     /locus_tag="L2X49_000713"
     CDS             complement(48114..48434)
                     /gene="psiF"
                     /locus_tag="L2X49_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBN4795853.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(48553..49968)
                     /gene="phoA"
                     /locus_tag="L2X49_000714"
     CDS             complement(48553..49968)
                     /gene="phoA"
                     /locus_tag="L2X49_000714"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBN4795854.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(50069..50329)
                     /gene="iraP"
                     /locus_tag="L2X49_000715"
     CDS             complement(50069..50329)
                     /gene="iraP"
                     /locus_tag="L2X49_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBN4795855.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT        12215 a        13440 c        12776 g        11946 t
ORIGIN      
        1 cgcccagact ccttctgcac tccccttttg ttataacgcc cttttgcaac agcttcttaa
       61 aatcaacctg atatgttttg caacatatgt gacctgacag ccaaatccaa gtaacaggaa
      121 tttaatcatg tttacagtaa tgtaaccttc ccgtaaaatg cccacacact ttaaacgcca
      181 ccagatcccg tggaattgag gtcgttaaat gagactcagg aaatacaata aaagtttggg
      241 atggttgtca ttatttgcag gcactgtatt gctcagtggc tgtaattctg cgctgttaga
      301 tcccaaagga cagattggtc tggagcaacg ttcactgata ctgacggcat ttggcctgat
      361 gttgattgtc gttattcccg caatcttgat ggctgttggt ttcgcctgga agtaccgtgc
      421 gagcaataaa gatgctaagt acagcccgaa ctggtcacac tccaataaag tggaagctgt
      481 ggtctggacg gtacctatct taatcatcat cttccttgcg gtactgacct ggaaaaccac
      541 tcacgctctt gagcctagca agccgctggc acacgacgag aagcccatta ccatcgaagt
      601 ggtttccatg gactggaaat ggttcttcat ctacccggaa cagggcattg ctaccgtgaa
      661 tgaaatcgct ttcccggcga acactccggt gtacttcaaa gtgacctcca actccgtgat
      721 gaactccttc ttcattccgc gtctgggtag ccagatttat gccatggccg gtatgcagac
      781 tcgcctgcat ctgatcgcca acgaacccgg tacttatgac ggtatctccg ccagctacag
      841 cggcccgggc ttctcaggca tgaagttcaa agctattgca acaccggatc gcgccgcatt
      901 cgaccaatgg gtcgcaaaag cgaaacagtc gccgaactcc atgtctgaca tggcagcgtt
      961 cgaaaaactg gccgcgccta gcgaatacaa ccaggtggaa tatttctcca acgtgaaacc
     1021 agacttgttt gccgatgtga ttaacaagtt tatggctcac ggtaagagca tggacatgac
     1081 ccagccagaa ggtgagcaca gcgcacacga aggtatggaa ggcatggaca tgagccacgc
     1141 ggaatccgcc cattaaaggg gttgaggaag aataaagatg ttcggaaaat tatcacttga
     1201 tgcagtcccg ttccatgaac ctatcgtcat ggttacgatc gctggcatta ttttgggagg
     1261 tctggcgctc gttggcctga tcacttactt cggtaagtgg acctacctgt ggaaagagtg
     1321 gctgacctcc gtcgaccata aacgcctcgg tatcatgtat atcatcgtgg cgattgtgat
     1381 gttgctgcgt ggttttgctg acgccattat gatgcgtagc cagcaggctc ttgcctcggc
     1441 gggcgaagcg ggcttcctgc cacctcacca ctacgatcag atctttaccg cgcacggcgt
     1501 gattatgatc ttcttcgtgg cgatgccttt cgttatcggt ctgatgaacc tggtggttcc
     1561 gctgcagatt ggcgcgcgtg acgttgcgtt cccgttcctc aacaacttaa gcttctggtt
     1621 taccgttgtt ggtgtgattc tggttaacgt ttctctcggc gtgggcgaat ttgcgcagac
     1681 cggctggctg gcctatccac cgctatcggg aatagagtac agtccgggag tcggtgtcga
     1741 ttactggata tggagtctcc agctatccgg tataggtacg acgcttaccg gtatcaactt
     1801 cttcgttacc attctgaaga tgcgcgcacc gggcatgacc atgttcaaga tgccagtatt
     1861 tacctgggca tcactgtgcg cgaacgtact gattattgct tccttcccaa ttctgacggt
     1921 taccgtcgcg ttgttgaccc tggatcgcta tctgggcacc catttcttta ccaacgatat
     1981 gggtggcaac atgatgatgt atatcaacct gatttgggcc tggggccacc cggaagttta
     2041 catcctgatc ctgcctgttt tcggtgtgtt ctccgaaatt gcggcaacct tctcgcgtaa
     2101 acgtctgttt ggttatacct cgctggtatg ggcaaccgtc tgtatcaccg tgctgtcgtt
     2161 catcgtctgg ctgcaccact tctttacgat gggtgcgggc gcgaacgtaa acgccttctt
     2221 tggtatcacc accatgatta tcgccatccc gaccggggtg aagatcttca actggctgtt
     2281 caccatgtat cagggccgca tcgtgttcca ttctgcgatg ctgtggacta tcggttttat
     2341 cgtcaccttc tcggtgggcg gtatgactgg cgtgctgctg gcagtaccgg gcgcggactt
     2401 cgttctgcat aacagtctgt tcctgattgc gcacttccat aacgtgatca tcggcggcgt
     2461 ggtcttcggc tgcttcgcag ggatgaccta ctggtggcct aaagcgttcg gtttcaaact
     2521 gaacgaaacc tggggtaaac gcgcgttctg gttctggatc atcggcttct tcgttgcctt
     2581 tatgccactg tatgcgctgg gcttcatggg tatgacccgc cgtttgagcc agcagattga
     2641 cccgcagttc cacaccatgc tgatgattgc agccagcggt gcagtactga ttgcgctggg
     2701 tattctctgc ctcgttattc agatgtacgt ttctattcgt gaccgcgacc agaaccgtga
     2761 cctgactggc gacccgtggg gtggccgtac gctggagtgg gcaacctctt ccccgcctcc
     2821 gttctataac tttgccgttg tgccgcacgt tcacgagcgt gatgcattct gggaaatgaa
     2881 agagaaaggc gaagcgtaca aaaagcctga ccactatgaa gaaattcata tgccgaaaaa
     2941 cagcggtgca ggtatcgtca ttgcagcttt ctccaccatc ttcggtttcg ccatgatctg
     3001 gcatatctgg tggctggcga ttgttggctt cgcaggcatg atcatcacct ggatcgtgaa
     3061 aagcttcgac gaggacgtgg attactacgt gccggtggca gaaatcgaaa aactggaaaa
     3121 ccagcatttc gatgagatta ctaaggcagg gctgaaaaat ggcaactgat actttgacgc
     3181 acgcgactgc ccacgcgcac gaacacgggc accacgatgc aggcggaacc aaaatcttcg
     3241 gattttggat ctacctgatg agcgactgca ttctgttctc tatcttgttt gctacctatg
     3301 ccgttctggt gaacggcacc gcaggcggcc cgacaggtaa ggacattttc gaactgccgt
     3361 tcgttctggt tgaaactttc ttgctgttgt tcagctccat cacctacggc atggcggcta
     3421 tcgccatgta caaaaacaac aaaagccagg ttatctcctg gctggcgttg acctggttgt
     3481 ttggcgccgg atttatcggg atggaaatct acgaattcca tcacctgatt gttaacggca
     3541 tgggtccgga tcgcagcggc ttcctgtcag cgttctttgc gctggtcggc acgcacggtc
     3601 tgcacgtcac ttccggtctt atctggatgg cggtgctgat ggtacaaatc gctcgtcgcg
     3661 gcctgaccag cactaaccgt acccgcatca tgtgcctgag cctgttctgg cacttcctgg
     3721 atgtggtttg gatctgtgtg ttcactgttg tttatctgat gggggcgatg taatgagtca
     3781 ttctaccgat cacagcggcg cgtcccatgg cagcgtaaaa acctacatga caggctttat
     3841 cctgtcgatc attctgacgg tgattccgtt ctggatggtg atgacagggg ctgcctctcc
     3901 ggccgtaatt ctgggaacaa tcctggcaat ggcagtggta caggttctgg tgcatctggt
     3961 gtgcttcctg cacatgaata ccaaatcaga tgaaggctgg aacatgacgg cgtttgtctt
     4021 caccgtgcta atcatcgcta tcctggttgt aggctccatc tggattatgt ggaacctcaa
     4081 ctacaacatg atgatgcact aagagcggcg gttatgatgt ttaagcaata cctgcaagta
     4141 acgaaaccag gcatcatctt tggcaacctg atctcggtga ttgggggatt cctgctggcc
     4201 tcaaagggca gcattgatta tcccctgttt atctacacgc tggttggggt gtcactggtt
     4261 gtggcgtcgg gttgtgtgtt taacaactac atcgacaggg atatcgacag aaagatggaa
     4321 aggacgaaga atcgggtact ggttaaaggc ctgatctctc ctgctgtctc gctggtgtac
     4381 gccaccttgc taggctttgc tggctttatg ctgctgtggt ttggcgcgaa tccgctggcc
     4441 tgctggctgg gggtgatggg ctttgtcgtt tatgtcggcg tttatagcct gtacatgaaa
     4501 cgccactcag tctacggcac attgattggt tcgctctccg gcgctgcgcc gccggtgatt
     4561 ggctactgtg cagtaaccgg cgagttcgat agcggcgcag cgatcctgct ggctatcttc
     4621 agcctgtggc agatgcctca ctcctatgcc atcgccattt tccgctttaa ggattatcag
     4681 gcggcgaaca ttccggtatt gccagtggta aaaggcattt cggtggcgaa gaatcacatc
     4741 acgctgtata tcatcgcctt tgccgttgcc acgctgatgc tctctcttgg cggttacgct
     4801 ggatataaat atctggtggt cgccgcggcg gttagcgtct ggtggttagg tatggctctg
     4861 cgcggttata aagttgctga tgacagaatc tgggcgcgca agctgttcgg cttctctatc
     4921 atcgccatca ctgccctctc ggtgatgatg tccgttgatt ttatggtacc ggactcgcat
     4981 acgctgctgg ctgctgtgtg gtaacaaaac ctctcagtta aaaaggtgct acggcacctt
     5041 ttttcttagc attagaaaca tatccctctc gaaatattta ctaaaaaatc cgcatgttta
     5101 ccccattcgt ttgccgcttt acactagtcg cgaatttaaa acagaggtgg taatgaacga
     5161 ttataaaatg acgccaggtg agcggcgcgc gacctggggt ttaggaaccg ttttctcgtt
     5221 gcgcatgctg ggcatgttta tggttctgcc ggttctgacc acgtatggca tggctctgca
     5281 aggtgccagc gaagcattaa tcggtattgc cattggtatt tatggtctga ctcaggccgt
     5341 ttttcagatt ccgtttggcc tgctttccga ccgcattggt cgcaaaccat taattgtcgg
     5401 tgggctggcg gtgtttgccg ccggtagcgt tatcgctgcg ctttctgact ccatctgggg
     5461 aattattctg ggccgggcgc tacaaggctc cggtgcgatt gccgctgccg ttatggcgct
     5521 gctttccgat ctcacgcgcg aacaaaaccg taccaaagca atggcgttta tcggcgtgag
     5581 ctttggcatt acctttgcca ttgcgatggt gcttggcccg atcatcactc acaaacttgg
     5641 gctgcacgcg ctgttctgga tgatcgctat tctggcaaca accggcattg cgttgaccat
     5701 ttgggttgtg cccaacagta gcactcacgt acttaatcgt gagtccggaa tggtgaaagg
     5761 cagtttcagt aaagtgctgg cggaaccgcg gctgctaaaa ctcaactttg gcattatgtg
     5821 tctgcacatg cttctgatgt ctacgtttgt tgccctgccc ggacagctag ctgatgcggg
     5881 gttcccggcg gctgaacact ggaaggtcta tctggcgacg atgttaatcg cctttggctc
     5941 ggtcgtgcct ttcattatct acgctgaagt taagcgcaaa atgaagcaag tctttgtctt
     6001 ctgcgtcggg ttgatcgtgg ttgcggaaat tgtgttgtgg aacgcacaaa cgcagttctg
     6061 gcaactggtg gtcggcgtgc agcttttctt tgtggcgttt aatttgatgg aagcccttct
     6121 gccctcactt atcagtaaag agtcgccagc aggttacaaa ggtacggcga tgggtgtcta
     6181 ctccaccagc cagtttcttg gcgtggcgat tggcggttcg ctgggcggct ggattgacgg
     6241 catgtttgac ggtcaggggg tatttctcgc tggcgcaatg ctggccgcag tgtggctggc
     6301 agtcgccagt accatgaaag aaccgccgta tgtcagcagt ttgcgcattg aaatcccggc
     6361 ggacattgcc gcaaacgaag cgttaaaagt gcgtttgctg gaaactgaag gcgtcaaaga
     6421 agtgttgatt gcagaagaag agcattcagc ttatgtgaaa atcgacagca aagtgacaaa
     6481 tcgctttgag gttgaacagg caattcgtca ggcataaaaa acgcggcgaa ttgcctgctg
     6541 cgctacgctt accaggccta caaagttctt gagttacaaa ccgttgtagg ccgaatcagg
     6601 cgttcatgcc gcatctgaca tgaacaaaac gcacattgtc gcgattaatc gcggaagttt
     6661 ttgaactgga acggctggcc gagatcgcca ccacgtacca tcgccatgac agcctgcaaa
     6721 tcatcacgag atttgccagt cacacggatc tcatcgccct gaatttgcgc ctgcactttc
     6781 agtttgctgt ctttaatcat cttgacgatt ttcttctggg tcgcgctctc aatgccctgt
     6841 ttcagtttcg cttccacaaa ccaggtttta ccgctatgaa cgatattttc cggtacatcc
     6901 agtgaactgc cttcaatgcc gcgcttcagc agcttggcac gcagaatatc cagcaactga
     6961 ttgacctgga agtcggactc gctcaacact ttgatggttt tgctggcgtc gttcagctca
     7021 aatgaggctt caacgttacg gaagtcaaaa cgggactcca cttcgcgact cgcgttatcg
     7081 accgcgttac gtgcttcctg aagatcaact tcagagacaa tatcgaaaga tggcatcttt
     7141 tcctctccct tcatttttga tgcgaagcat aatacctgca aagttaaata accaacagtc
     7201 ctgcaccagg acgtatccct taattgtgca tagtcgcaac agcatgcaca attaagggat
     7261 agccttattg aacacccgga gtggttgcgg gtgaggagga acaatgaaaa ttaccgtatt
     7321 gggatgcggt gccttagggc aattatggct tacagcactt tgtaaacagg gtcacgaagt
     7381 tcagggctgg ctgcgcgtac cgcaacctta ttgtagtgtg aatctggttg agacagatgg
     7441 ttcgatattt aacgaatcgc tgaccgccaa cgatcccgat tttctcgcca ccagcgatct
     7501 gctcctggtg acgctgaaag catggcaggt ttccgatgcc gtcaaaagcc tcgcatccac
     7561 actgcctgta gctacgccaa tactgttaat tcacaacggc atgggcacca tcgaagaatt
     7621 gcaaaacatt cagcagccat tactgatggg caccaccacc catgccgccc gccgcgacgg
     7681 caatgtcatt attcatgtgg caaacggaat cacgcatatc ggtccggcac ggcaacagga
     7741 tggcgattac agttatctgg cggatatttt gcaaaccgtg ttgcctgacg ttgcctggca
     7801 taacaatatt cgcgccgagc tgtggcgcaa gctggccgtc aactgtgtga ttaatccact
     7861 gaccgccatc tggaattgcc cgaatggtga attacgtcat catccgcaag aaattatgca
     7921 gatatgcgaa gaagtcgcgg cagtgatcga gcgcgaaggg catcatactt cagcagaaga
     7981 tttgcgtgat tacgtgatgc aggtgattga tgccacagcg gaaaatatct cgtcgatgtt
     8041 gcaggatatc cgcgcgctgc gccacaccga aatcgactat atcaatggtt ttctcttacg
     8101 ccgcgcccgc gcgcatggga ttgccgtacc ggaaaacacc cgcctgtttg aaatggtaaa
     8161 aagaaaggag agtgaatatg agcgcatcgg cactggtttg cctcgcccct ggtagtgaag
     8221 agactgaagc cgtcaccact atcgatctgc tggttcgcgg cggtatcaaa gtcaccactg
     8281 ccagcgtcgc cagcgatggc aacctggcca ttacctgctc gcgcggcgtg aagctgctgg
     8341 cggatgcgcc gctggtcgag gtggctgatg gcgaatatga cgtgatcgta ctgcctggtg
     8401 gcattaaagg cgcagagtgt tttcgcgata gtaccctgct ggttgaaacc gttaaacagt
     8461 tccaccgttc cgggcgtatc gtcgcggcta tttgcgccgc gccagccacc gtgctggtgc
     8521 cgcacgatat cttcccgatt ggtaatatga ccggcttccc gacgctgaaa gacaaaattc
     8581 ccgctgaaca atggcaggac aagcgcgtcg tctgggatgc acgggtaaaa ttgctgacca
     8641 gccaggggcc ggggacagct atcgactttg gtctgaaaat tatcgacctg ctggttgggc
     8701 gcgagaaagc ccatgaagtg gcatcacaac tggtgatggc ggcagggatt tataattatt
     8761 acgagtagtg tcggatgcgg taagcgtcgc atctgaccag atgcgacgtt acaaccacaa
     8821 attacgggcg atataccttc acattgttaa agccctgctc gcgcagatag agcgcctgca
     8881 gacggctcat caccccgcgc tcacaccaca gtagccaggt tctgttctgg tcgagatcgc
     8941 caaacttggt gctcagttta tagaacggca gagaaactac atcaatccct tcgactttca
     9001 gtggcttatc ttcctgttca tcgatagaac ggatatcgag gatcacgtcg ttcgggccga
     9061 agccattgac ggtttccact tccaccactt cctgctcggt ctgctgggcg atttcgcgga
     9121 tatcaacgtt attcgcttct tcaaccactt tatcgagaat gctgaagtcg aatttctctt
     9181 cttccgcttc aatcttcgac ttaaccgctt tcaccgtcgg gcttttggag ataacaccgc
     9241 aatattccgg catcgtgcga gcaaagtctt cggtgccaat ctggcgagcc aggttgatga
     9301 tgtgctcttt gtcgtaagaa atcagcggac gcaggatcag cgtatcggag acgttatcaa
     9361 tcaggcgcag gttggtcagc gtctgactgg acacctggcc gagcgcttcg ccggtgacca
     9421 gcgcctgtac gccgtaacgt tcagccactt tagacgcggc acgcaccatc atacgtttga
     9481 ggataacgcc catctgaccg tcgtcgattt tctcgagaat ttccccgacg accggttcga
     9541 aattaatagc gacaaaacgc acgcggtggg agctgccaaa acggttccac agataatgcg
     9601 ccacctgacg cacgccaatt tcatgcgccg cgccgccaag gttaaagaaa cagtaatgca
     9661 cgcggcagcc gcgacgcatc aacatataac tggaaacacc ggagtcgaaa ccaccggaaa
     9721 tgagcgacag cacatcttcc tgggtgccga tcgggaaacc gccaatacct tcgtagcggc
     9781 ctttaatcag caggagacga tcgtcttcca cttccagatg gacagtcact tccggattgg
     9841 tcagcttcac gcgcgcggac tcaatatgct gatttaaacc gccgccgacg taacgttcca
     9901 catcaatcga gctaaaatca tgtttgccac ggcgcttcac gcgtacgcag aaggttttgc
     9961 cttccagctg atcgcgatac tgaaccaacg ctttctcaaa aatatcgtgc atgtcggtaa
    10021 acggcacgtc ttcgacttcg agaatatggt ggatacccgg aatacgggtc agggcgtcgc
    10081 gaatagccag acgctggttt tcatcttttg cgcgaacttc gatgttatcc cagtggcgga
    10141 cgacagcgag cgtctcatca tagtgcttta aaacgttacg aatgttcccg gtaaggattt
    10201 ttataaagcg caagcgcaca gattggcttt tgatggtgat ttccgggaac aatttaatga
    10261 taaacttcat ggcggcaatg gttcgttggc aagccttaag cgacttgtat agggaaaaat
    10321 acagcagccc acacctgcgg ctgcatccag gcgcggaagt ataccactaa catcgctttg
    10381 ctgcgcacat caccttacca ttgcgcgtta tttgctattt gccctgagtc cgttaccatg
    10441 acggggcgaa aaatattgag agtcagacat tcattatgcc gaagaaaaat gaggctcccg
    10501 ccagctttga aaaggcgctg agcgagctgg aacagattgt aacccgtctg gaaagtggcg
    10561 acctgccgct ggaagaggcg ctgaacgagt tcgaacgcgg cgtgcagctg gcacgtcagg
    10621 ggcaggccaa attacaacaa gccgaacagc gcgtacaaat tctgctgtct gacaatgaag
    10681 acgcctctct aacccctttt acaccggaca atgagtaatg gactttccgc agcaactcga
    10741 agcctgcgtt aagcaggcca accaggcgct gagccgtttt atcgccccac tgccctttca
    10801 gaacactccc gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta agcgtctgcg
    10861 acccttcctg gtttatgcca ccggtcatat gtttggcgtt agcacaaaca cgctggacgc
    10921 acccgctgcc gccgtagagt gtatccacgc ttactcatta attcatgatg atttaccggc
    10981 gatggatgat gacgatctgc gtcgcggttt gccgacctgc catgtgaagt ttggtgaagc
    11041 aaacgcgatt ctcgctggcg acgctttaca aacgctggcg ttctcgattc taagcgatgc
    11101 caatatgccg gaagtgtcgg atcgcgacag aatttcgatg atttctgaac tggcgagcgc
    11161 cagcggtatt gccggaatgt gcggtggtca ggcattagat ttagacgcgg aaggcaaaca
    11221 cgtacctctg gacgcgcttg agcgtattca tcgtcataaa accggcgcat tgattcgcgc
    11281 cgccgttcgc cttggtgcat taagcgccgg agataaaggg cgtcgtgctc tgccagtact
    11341 cgacaagtac gcagagagca tcggccttgc cttccaggtt caggatgaca tcctggatgt
    11401 ggtaggagat actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac ttggtaaaag
    11461 tacctaccct gcacttctgg gtcttgagca agcccggaag aaagcccggg atctgatcga
    11521 cgatgcccgc cagtcgctga aacaactggc tgaacaatca ctcgatacct cggcactgga
    11581 agcgctagcg gactacatca tccagcgtaa taaataaaca ataagtatta ataggcccct
    11641 gatgagtttt gatattgcca aatacccgac cctggcactg gtcgactcca cccaggagtt
    11701 acgactgttg ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc gctatttact
    11761 cgacagcgtg agccgttcca gcgggcactt cgcctccggg ctgggcacgg tcgaactgac
    11821 cgtggcgctg cactatgtct acaacacccc gtttgaccaa ttgatttggg atgtggggca
    11881 tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca ccatccgtca
    11941 gaaaggcggc ctgcacccgt tcccgtggcg cggcgaaagc gaatatgacg tattaagcgt
    12001 cgggcattca tcaacctcca tcagtgccgg aattggtatt gcggttgctg ccgagaaaga
    12061 aggcaaaaat cgccgcaccg tctgtgtcat tggcgatggc gcgattaccg ctggcatggc
    12121 gtttgaagcg atgaatcacg cgggcgatat ccgtcctgat atgctggtgg tcctcaacga
    12181 caatgaaatg tcgatttccg aaaatgtcgg cgcgctcaat aaccatctgg cacagctgct
    12241 ttccggtaag ctttactctt cgctgcgcga aggcgggaaa aaagttttct ctggcgttcc
    12301 gccaattaaa gagctgctca aacgtaccga agaacatatt aaaggcatgg tagtgcctgg
    12361 cacgttgttt gaagagctgg gctttaacta catcggcccg gttgacggtc acgatgtgct
    12421 ggggcttatc accacgctga agaacatgcg cgacctgaaa ggcccgcagt tcctgcatat
    12481 catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa gaccccatca ctttccacgc
    12541 cgtgcctaaa tttgatccct ccagcggttg tttgccgaaa agtagcggcg gtttgccgag
    12601 ctattcaaaa atctttggcg actggttgtg cgaaacggca gcgaaagaca acaagctgat
    12661 ggcgattact ccggcgatgc gtgaaggttc cggcatggtc gagttttcac gtaaattccc
    12721 ggatcgttac ttcgacgtgg caatcgccga gcaacacgcg gtgacctttg ccgccggtct
    12781 ggcgattggt gggtacaaac ccattgtcgc gatttactcc actttcctgc aacgcgccta
    12841 tgatcaggtg ctgcatgacg tggcgattca aaagctcccg gtcctgttcg ccatcgaccg
    12901 cgcgggcatt gttggtgctg acggtcaaac ccatcagggc gcttttgacc tctcttacct
    12961 gcgctgtata ccggaaatgg tcattatgac cccgagcgat gaaaacgaat gtcgccagat
    13021 gctctatacc ggctatcact ataacgacgg cccgtccgcg gtgcgctacc cgcgcggtaa
    13081 cgcggttggc gtggaactga cgccgctgga aaaactgcca attggcaaag gcattgtgaa
    13141 gcgtcgtggt gagaaactgg cgatccttaa ctttggtacg ctgatgccag aagcggcgaa
    13201 agtcgccgaa tcgctgaacg ccacgctggt cgatatgcgt tttgtgaaac cgcttgatga
    13261 agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag aagaaaacgc
    13321 cattatgggc ggcgcaggta gcggagtgaa cgaagtgctg atggcccatc gtaaaccagt
    13381 acccgtgctg aacattggtc tgccggactt ctttattccg caaggaactc aggaagaaat
    13441 gcgcgccgaa ctcggcctcg atgccgccgg tatggaagcc aaaatcaagg cctggctggc
    13501 ataatcccta ctccactcct gctatgctta acaaattatt catagactct aaataattcg
    13561 agttgcagga aggcggcaaa cgagtgaagc cccaggagct tacataagta agtgactggg
    13621 gtgaacgaat gcagccgcag cacatgcaac ttgaagtatg acgagtatgg caggagtggc
    13681 agcatgcaat acaacccctt aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc
    13741 tgtatgacct ttggcgagcc agatcgtggt aatcacgcat ggacactgcc ggaagagagt
    13801 agccgtccca tcattaaacg cgcgctggaa ggcggcataa atttctttga taccgccaat
    13861 agcttttccg atggcagcag cgaagagatc gttggtcgcg cactgcggga tttcgcccgt
    13921 cgtgaagacg tggtcgttgc gacgaaagtg ttccatcgcg ttggtgattt accggaagga
    13981 ttatcccgtg cacaaatttt gcgctctatc gacgacagcc tgcgccgtct cggcatggat
    14041 tatgtcgata tcctgcaaat tcatcgctgg gattacaaca cgccgatcga agagacgctg
    14101 gaagccctga acgacgtggt aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg
    14161 cacgcttcgc agtttgctca ggccctgaag ctacaaaaac agcacggctg ggcgcagttt
    14221 atcagtatgc aggatcacta caatctgatt tatcgcgaag aagagcgcga gatgctgcct
    14281 ctgtgttatc aggagggcgt ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg
    14341 acgcgtccgt ggggagaaac taccgcacga ctggtgtctg atgaggtggg gagaaatctc
    14401 tataaagaaa gcgatgaaaa tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa
    14461 gaactcggtg caacacgagc acaagttgcg ctggcctggt tgttgagtaa accgggcatt
    14521 gccgcaccga ttatcggtac atcgcgggaa gaacagcttg atgagctgct gaacgcggtg
    14581 gatatcactt tgaagccaga acagattgcc gaactggaaa cgccgtataa accgcatgcg
    14641 gtcgtaggat ttaaataaga gtccaggcct gatgagacgt gacaagcgtc acatcaggca
    14701 tcggtgcaca actacgacag aatacccagc ggccagtgat gaccgataaa gtacaggatg
    14761 cctgcggaaa tcaccccggc gacaatatcg tcgatcatga tccccatgcc gccatgtaca
    14821 ttgcgatcaa accagcggat cggccacggc ttccacatat ccagaatacg gaaaatcaca
    14881 aacccggcgg caacccactg ccagtcattg gtcggcagcg ccatgagcgt gatccacata
    14941 ccaataaatt cgtcccagac aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt
    15001 tgatgacaaa gatagacgcc gatacagatc cccaacatca ccaccagcga gtagagctgc
    15061 cagggtaaaa aggtcatcag ataccagaac ggaatcgctg ccagcgatcc catcgtgcca
    15121 ggaacgattg ggcttaatcc acttccgaat ccgacagcaa gcagatgcca cggattactc
    15181 atcttcaggc gacttttcgc gacatcttta tggcgtggca aaatggtcat atcctttcca
    15241 gtcaaacgtg acaggttcgc cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat
    15301 ttgcccgata caggtaaacg gtacgcccag gtgtccgaga gccacatcca gcgcgccacg
    15361 gttcagttcc ggcacagtga aacacaactc gtaatcttca ccgccagaga gcgcccagcg
    15421 cagcgcctgt tccggttcaa catggcgaga aagcgcatca gaaaacggca gcaatgccag
    15481 gtcaatacgt gcgccgcagt cgctggcttt cacgatatgc ccgagatcgg aaatcagacc
    15541 gtcagagaga tcgatagctg aatttgccag atcgcgcagt gcctgtccct gtaaaatacg
    15601 cggcgatgga cggagatgac gtttgatcaa gtagtccgca tctttagcat cggcaacctg
    15661 caaacggttt tgcaaaatcg ccagcccggc ggcgctatcg cccggtgtac cggtcacata
    15721 gatccagtca cccggtttcg ctccagagcg cgttaaggct cgtcccatcg gaacaaagcc
    15781 gtggataccc aacgtcattg ataatggccc acgcgtggta tcgccgccaa tgagttgcat
    15841 atcgtaataa ttgagaagat caaacaaact gtcgctgaag gactcaagcc acgcttcgtc
    15901 tacgtccggt aaggttaatg ccagcgtcag ccaggccgga tcggccccca tcgctgccag
    15961 atcgcttagg ttcaccgcca gtgctttata cgccagatca gcaggatcga tatcatggag
    16021 gaagtggttg cccgccacca gcgtatcagt gctgatcgcc agggtctgtt tctcggggat
    16081 attgagaagt gcgcaatcgt cgccgatgcc cagttcgaca tcaagacgag aacttcttac
    16141 acggtcaaaa taacgggcaa tcagggagaa ctcgccacat gccatacgtt atgcctcagc
    16201 aggtaaaaaa gaaaaggccg gcgatcatgg aacggtcttc cgtgaatcta ccggcctgga
    16261 tattactttt tgttagggcg aatcacaggt gctgctttat cgagtacgcc gttgacgaac
    16321 ttatggctgt cttctgcgcc gaacgatttc gccagttcga tcgcttcgtt aatggccact
    16381 ttgtatggca catcgctacg tttagacagc tcgtacagcg caatgcgcag tactgctttt
    16441 tctacctgtc ccagttcttc cagcaggcgg gacaggtatg gcttcatcag tccgtcgagg
    16501 tatgcggtgt tagtcgccac cccggccagc agctcacgga agtacaggac gtcaacatct
    16561 tttacatcct gttcagccag gaactggtat tcaacatcag cgatgtcgtt ctgggacaac
    16621 tgccaggagt agagcgcctg gacggcacac tcacgagcgc ggcgacgagc agcaggtttc
    16681 acggatttcc ccttactaat ttcaggcctt gatggctttc aatacattaa tcatttcaag
    16741 cgcggtcagt gcagcttctg cacctttgtt gccagctttg gtgccagcac gttcgatcgc
    16801 ttgttcaatg ctttcagtgg tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc
    16861 aacatgcgcc aggccgttgc ttgcaccacc agcgacatat tcaaaatggg cagtgccacc
    16921 acgaataacc gtacccagcg caatcaccgc gtcgtattta ccggttttag ccagtgcacc
    16981 cgccgccagc ggcagctcat aggcaccagg cacccaaaca acggtaatgt tttcatcttt
    17041 tacctgaccg atacgtttca gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa
    17101 gttgttgaaa cgcgcgatgg tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc
    17161 aataatgttc attttcttcc ttcgggttcg agtatggccc cgcagggggg cggattttag
    17221 cataatattt cgtgcgctgc ttccctttcg agccgggaga tcatgcaccc actaaatgca
    17281 ggcaaacatc cgggcctaca tgacgtatct ctttgaattt aaattggggg gcgtcggcta
    17341 atttctcaag ccctggcagc gtgcataatc cgcgggcgtc gctgcctaat agtttaggtg
    17401 cgatatagac aatcagctca tcgactaaac ccgcctgcag caatgcgcca gcgagcgttg
    17461 gccccgcttc cacccagatg ctgttaattt gctgtttacc cagttgcatc atcagtacaa
    17521 ccagatccag atgaccttta tgctctggaa tcagcaaggt acgcaccgtt tccggccact
    17581 cactagaatc ttcctgcgta cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat
    17641 gttccggcgt cacgcgattt tggctatcaa tcacaatacg tactggctgg cggagatttt
    17701 gttgcgggta gagcgcctga gtttgttcat ccagttcaga ccaacgcact gttaaggctg
    17761 gatcatccgc cagcaccgtg gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc
    17821 gttgtacgtc gcgccgcgcc tgaggcgaag tgatccactg gctttcgccg ctcgccatcg
    17881 ccgtgcggcc atcaagcgat gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca
    17941 tccgcttgag aaagccttta ttcaattgct cggcttcact catcatcagc ccgtggctga
    18001 cgtcaatgcc agcctgttgc agacggtaaa gtccacgccc agcgacctgc gggttaggat
    18061 cttgcatcga ggcaaccacg cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg
    18121 gcgtacgacc atgatggcta cagggttcga gtgtgacata ggcggtcgca cctttggctt
    18181 tttcacccgc catacgcaac gcgtgtactt cggcatgtgg ttcacccgca cgttggtggt
    18241 aaccttcacc gacaatttcg ccatctttga caatgacgca cccgacattc gggttgggat
    18301 gcgtggtaaa acgtcctcgt tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct
    18361 gcacggctta gtcctccagg cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac
    18421 tgcggtagac agaggcaaaa cggatatagg cgactttatc gagctttttc aattgctcca
    18481 tcaccagatt gccaatcatc ttgctcggca cttcgcgctc accggtggcg cgcagctgcg
    18541 atttaatatg attgattgcc atttcgacgt catcggaact caccggacgt ttttccagcg
    18601 cccgcagcat tccgctacgc aatttctctt cattaaacgg ttcacgcacg tcgttgcttt
    18661 ttacaacacg cggcataacc agctccgcca cttcaaaggt ggtgaaacgt tcattacaca
    18721 ccagacactg ccgacggcgg cgtacggatg aaccctcgcc cacgagacga gagtcaatta
    18781 ccttagtgtc cacggcgaaa cagaatgggc aatgcatacg gcgtcctgac caggtggtta
    18841 acagaaatct attttaccct gaactgacga tacaacaaag gcgcaacgct tttgagataa
    18901 aggatcgatg ctttcgctgc ttttaccctc taccattagg ctaattgcga cgagaaaggg
    18961 acgacctatg aatactaacg tttttcgact gctcctgctg ggaagcctgt tcagccttag
    19021 cgcctgtgtg cagcagagcg aagtgcgaca gatgaaacac agcgtcagca cgctgaacca
    19081 agagatgacg caactcaata aagaaacggt caaaatcact cagcaaaaca ggctgaatgc
    19141 aaaatccagc agtggggttt accttctgcc aggagcgaaa acaccggcaa gactggaaag
    19201 ccagatcggt actttacgta tgtcgctggt gaatattacg cctgatgcag atggcaccac
    19261 cctgacgcta cgtattcagg gcgagtcgaa tgatcccttg cccgcattca gcggaactgt
    19321 tgaatatggg caaattcagg gaacaataga caactttcag gaaatcaatg tgcagaatca
    19381 attgattaat gcccctgcca gcgtcctcgc ccccagcgat gttgatattc cgttacagtt
    19441 aaagggtatt tctgtagaac agttagactt cgtacgtatc cacgatattc aacctgttat
    19501 gcagtaaacg tatttcgggg cgattttgtg cgtctcgcaa catctttccc cgtcattttg
    19561 ttactccgct tacatcaccc tgattgatag taaaagtttg caacaagggt gaaagtcagt
    19621 acaatccctg cccgaatgtg tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact
    19681 gtgaaacgaa acatattttt gtgagcaatg atttttataa taggctcctc tgtatacgaa
    19741 atatttagaa acgcaatttg cacctttttc actcccgcaa gggattttca aacagtggca
    19801 tacatatgaa aaaaacatta ctggcagccg gtgcggtact ggcgctctct tcgtctttta
    19861 ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc cgactggtgg caccagagcg
    19921 ttaacgttgt cggaagctat cacacccgtt tcggaccgca gatccgcaac gatacctacc
    19981 ttgagtacga agcattcgct aaaaaagact ggttcgactt ctatggttat gcggatgcgc
    20041 cggtattctt cggcggtaac tccgatgcta aaggtatctg gaaccacggt tctccgctgt
    20101 ttatggaaat cgaaccacgt ttctccatcg acaagctgac caatactgac cttagcttcg
    20161 gtccgttcaa agagtggtac ttcgcgaaca actacattta cgacatgggt cgtaataaag
    20221 atggtcgcca gagcacctgg tacatgggtc tgggtaccga catcgatact ggtctgccga
    20281 tgagcctgtc catgaacgtc tatgcgaaat accagtggca gaactatggc gcagcgaacg
    20341 aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt
    20401 ggggcggtca gctgagctac atcggcttca ccaacttcga ctggggttcc gatttagggg
    20461 atgacagcgg taacgcaatc aacggtatta agacccgtac taataactct atcgcttcca
    20521 gccatattct ggctctgaac tacgatcact ggcactactc tgtcgtagct cgttactggc
    20581 acgacggtgg tcagtggaac gacgatgcag aactgaactt cggcaacggc aacttcaacg
    20641 ttcgctctac cggctggggt ggttacctgg ttgtaggtta caacttctga ttatgaaaat
    20701 gccgggattt attcccggca tttcagattg ttaacaacgt gcgctttgtt catgccggat
    20761 gcggcgtaaa cgctttgtcc gacccacaaa agcacgtaaa ttcaatatat tgctgaaata
    20821 acgtagtcct gataagcgta gcgcatcagg caattttacg ttttaatcac ttcttcttgt
    20881 tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg
    20941 catcttcccc tgcgataacg gtcgtaccat actgatccgc ttcggtatat ttcgaatgct
    21001 catgatcgtg gcaatagaga caaagcaatt cccagttact gccatcttcc gggttattgg
    21061 tatggtcgtg atcaatgtgg tgaacagtaa gttcacgcag gttggaataa acaaactcgc
    21121 gggaacagcg accgcagacc cacggatata tttttaatgc tttttcgcga tagccgcttt
    21181 ctaaccgcgc atagtttttt gggatgatag ccatgaagag ttacctggat gaaatgtagg
    21241 gttacgcgca gtttcccaag atgcggggat tttcgcaatc tccatacatc aaaaaaaatc
    21301 ccgatcttct gaccgggatt ttcaacatca acgggaactt gattacggca gaattgacgg
    21361 ctgatccgcc ccttcttttt ccactttctg ctgcaacatg tgttcgcgct tcatacccag
    21421 tttcagagcc aacgcagatg ccacatagat ggaagatgca gtaccgatgg aaacaccgat
    21481 aagcatggtc agcgagaagc cttccagtac cggaccaccg aagaggtaca gcatcaggat
    21541 aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac
    21601 gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg aagttttcac gaatacggtc
    21661 cgataccacg atactgtcgt taagcgagta accgataacc gacatcaacg atgccacaat
    21721 ggtcaggtca atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg
    21781 cgccagcgca ataacaaccc ctgccgccag tcgccactca aagcgaaaac ctacgtacac
    21841 aaggatagac agcagcgccg ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc
    21901 cacgctcgga ccgacgaact caatacgctt cactgctgca ttctgattgg tggattcgtt
    21961 aatcaccttc agaacctggc tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg
    22021 catacggacc atgatgtcgt ggctgctacc aaagttttgc agcattggct cttcaaaacc
    22081 ggctttttgc aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc
    22141 gataaccgta ccaccggtga aatccagccc ccagttaaag ccgcgcacgc ccataataac
    22201 gatagcggcg attaacagca gaccagagat gccgaaagcc cagtagtccc agcgcataaa
    22261 gtcatagact ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca
    22321 ctcctcagat tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac
    22381 gcgtaccgac aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga
    22441 accctttaat tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga
    22501 tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa
    22561 cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga
    22621 ccgccaccgc aagggttaag acgatacccg cgatacctgg catactcagc gttgcgcctg
    22681 gcaacagcga cataatgccg acgattaaga tcaagttggc aatcagagca ctggtcgcaa
    22741 tcagaccaaa cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa
    22801 ggcaagcctc cagcccctgt tcaatgttct gcatacccag ggttgggcca atggtacgtt
    22861 cttcaacaat ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gacagctgac
    22921 gggcttcgtt cgggttgttg atgccggtga tacggaagct gttacccaga cgagactgga
    22981 tgttggcgat gttaatcacc tcttcctgtt tcaccagaac cgcacgacca tttgcatctt
    23041 tcttaccgct gtctttgtac tccacaaaca gggttgccat cggtttgccg atgttgtcct
    23101 tagtgaagtt agacatgatg ttaccaccag cgctatcgag cgagatgtta acctgcggct
    23161 ggttgtattc gtcctggctg gaagtggagt cggtgatatg gtcaccggtc agaattacgc
    23221 gtttgtacag cacaactggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta
    23281 cgcgaccgga tgccgcagcg gcctggtcaa cgttggtgtt taccagacgg aattccagcg
    23341 ttgccgtcgc acccagaatc tctttcgcac gcgcagtgtc ctgaatacct ggcagttcaa
    23401 caacgatacg gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca agttggttta
    23461 cacggttacg caggatattg atgttctgct gcaccgcata ttcacgcgct tcactcagac
    23521 gagcatcgct cattaccgca cgcagctggt tgctgccctg gctgctaatt accaggtccg
    23581 gatggcgctt gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtaa
    23641 tgctcaggcc gtagttgttt tctttacgaa cggtggtata cgggatgccc ttttcacgca
    23701 gatcgctgcg caggctatcg atattttgtt cctggagttt gccaagcgcg gtgtccatat
    23761 ccacttccat caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag
    23821 cgtgaatagc tgccagccag cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt
    23881 tgtcacccat aacgcccatt aatgcttcac gagcgcgcag ctgggtgtca gtggagtcga
    23941 agcgcgcaag aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt
    24001 gtaacgtttt ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag
    24061 tgatctgaac agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga
    24121 caatcaccac gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc
    24181 ccttagggaa aaattttaat tacagcgcct tcatggtgcc tttcggcagg acggcagcta
    24241 cgaagtcacg tttaataact acttcagtgg tgtcattcag cgcgatagca atgtagccgt
    24301 tttccgctac tttggttacg cgaccaacca ggccaccgtt cgtcagaact tcatcacctt
    24361 tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga
    24421 tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgggctac
    24481 cttgcgccgg tgcacccgtt gccgctaccg catcagaaat aaaaaagctc attaaatttc
    24541 cctcattatt aatattaatc aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa
    24601 aaatcagtta cgaagctctc taatttaccc tcttcaatag ccttgcgtaa acccgccatc
    24661 aaacgctggt agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg
    24721 caacggtcaa gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta gcaatcacac
    24781 tcaggatcga gtgggccagt atcgctctta tacttcgcat tgcggatttt caccacgcca
    24841 tcggtcacga acaaatgacc atttcgggcg ttgcgggttg gcattacgca gtcaaacata
    24901 tcgataccac gacgtacgcc ttcaaccagg tcttctggtt taccaacgcc catcaggtaa
    24961 cgcggtttgt ctgccggaat ttgcgggcat acatgctcca gaatgcggtg catatctgct
    25021 ttcggctcac ccacagccag accgccgaca gcgtagccat caaaaccgat atctaccaga
    25081 cctttaacag aaatatcacg taaatcttcg taaacgctgc cctgaatgat accaaacagc
    25141 gcatttttgt ttccgagact gtcaaaacgc tcacggctac gcttcgccca acgcagagac
    25201 atctccatgg agcgttttgc gtaatcccag tcagcaggat acggcgtaca ctcatcaaag
    25261 atcatgacga tatccgaacc aagatcgtac tgaatctcca ttgatttttc aggatcgagg
    25321 aaaatcggat cgccgttgat cgggttacgg aagtgcacgc cctgttcggt gattttacga
    25381 atatcgccaa ggctgaagac ctggaagccg ccggagtcgg tgaggatcgg ccccttccac
    25441 tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg gcgcagccac
    25501 aggtggaagg tgttgccgag gataatttgc gcgccagtgg cttcaacttc ttccggcgtc
    25561 atccctttta cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc cactacgcca
    25621 cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc
    25681 attttttctc cactacgtca gaaaaacagt ccaacgtttt aaaccagcgc cgcggaatta
    25741 ctccccgacg cgctcattaa ttgcctgcgg attgtacgtg ataaacatcg catcaccgta
    25801 actaaaaaag cgatatttct cttctaccgc tgctttatag gcattcatgg tgtgttgata
    25861 accggcaaag gccgaaacca gcataatcag cgtcgattcc ggcaggtgga agtttgtcac
    25921 cagcgcatcg accactttgt actggaagcc cggatagata aagatttggg tatcgtcaaa
    25981 gaacggttcg ataagatcgt ttttcgccgc ctgagccgcg ctttccagcg aacgtactga
    26041 ggtggtgcca accgcaatca cccggttacc gcgcgctttc gccgccagta ccgcgtctac
    26101 cacatcctgc ggcacttcag cgtattccga gtgcatgatg tgatcttcaa tggtgtcgac
    26161 gcgcaccggc tggaaggtgc ccgcaccaac gtgcaacgtc acaaacgcca tctccacgcc
    26221 tttggcgcgt aatttttcca gcaaaggttc gtcaaaatgc agacctgcgg tcggggctgc
    26281 aaccgcgccc ggtttttcgc tataaacggt ttgataaagt tcgcggtcag cgtcttcgtc
    26341 cggacggtcg atatacggcg gcagcggcat atggccgatg ctgttgagaa tatccagcac
    26401 cgagcgttca tcattaaatt cgacttcaaa cagtgcgccg tggcgcgcgg tcattgttgc
    26461 gttaatactt tcgtcatcgc ccagcagcag ttctgcgcca ggttttggcg ctttcgaggc
    26521 gcgaatatgc gcaagaatgc gtttgtcgtc gagcatccgt tcaaccagca cttcaatctt
    26581 gccgccgctg gctttacgcc caaacaggcg cgccgggatc acgcgggtat tattaaaaac
    26641 cagaagatcg ccggggttga gcttatcaag taaatcggtg aaagtaccgt gcgtcagcgc
    26701 gcccgtcggc ccgtccagcg acagtaaacg acagctactg cgttcaggca tgggatagtg
    26761 ggcaatcagg gattcgggca attcaaagga gaaatcggta acgcgcatga cactgactca
    26821 gactaaaata agaggcgggt agtctagtgc cggggcgctc tccctgcaac ctttaccccc
    26881 ttttggatag aatataatcg atgaattttt tagctcacct gcatttagcc catctcgcgg
    26941 aaagctcgct ttccggcaat ttactggctg atttcgtacg cggaaatcct gaagaaagtt
    27001 ttccgcccga cgtcgtggct ggcattcata tgcatcgacg tatcgacgta ttaaccgaca
    27061 atctgccgga agtccgcgaa gcacgggaat ggtttcgtag tgaaactcgc cgcgttgcgc
    27121 ctattacgct ggatgtcatg tgggatcact ttctttcccg ccactggtcg cagctgtcgc
    27181 cggactttcc gctacaggaa tttgtctgtt atgcccgcga gcaagtgatg acgattttgc
    27241 cggactcacc gccacgtttt atcaatctga acaattactt gtggtcagag cagtggctgg
    27301 tgcgctatcg cgatatggat ttcatccaga acgtgttaaa cggcatggca agccgccgcc
    27361 cacgtctgga tgccctgcgt gactcctggt acgatttaga cgctcattat gacgccctcg
    27421 aaacccgctt ctggcagttt tatccgcgga tgatggcgca ggcgtcacac aaggcgttat
    27481 aaaacgttag ttcatccata ccgtagccga aatggcaggc aaagccagaa tcccgtcagt
    27541 cagttgccca tgcccttctt tgcattgcca ttgcacggca ttgagcaacg gtgacgcggg
    27601 tagcacgact tcacaagctt cgccacggtt gattgccacc agtacacgct gctgattcag
    27661 cacgcggaca aataccacca cgttatcttc cgcatacagc acctgacagc cgccatgacg
    27721 tagcgcctga cttttcttac gcagcgcaat cattcgctgg tacagcgcga ataacgccgt
    27781 atcctgcttt tccacctgcc aagggaacgg tttacggcaa aacggatcgt ttttgccatc
    27841 cagtcctact tcatcaccgt aataaatgca cggtacacca ggccaggtga acagccagac
    27901 caccgccagc ggcaggcgcg caatatcccg accgagcagc gttttaaatc gcgcagtatc
    27961 gtggctgtcg agctgattaa acatacgtaa ttgttgttga tgagaaagcc ctgcgcggta
    28021 attatccatc caggccatac aggtttgggc atcaatttgc tgcggatcgt aagagatatc
    28081 ggtattggca agaaatcccc acaacgggaa tgtgaagcca cgatagttca tggcggcatc
    28141 ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa tgttcgccga caatatacgc
    28201 ttccggctgg gtttctttcg ccgcttcggt gatcccagca acgtgctgca tattattgcg
    28261 cgccccaccc gcctccccca gcatatgcac cacatccagc cgccagccgt ccatactcca
    28321 cggcgctttc agccaatggc ggacaatact gtcttccccg cgataaattt cattcaccag
    28381 actttccgac tgataatcca gcttcggcaa gctggcatag ccaagccagt cgagcgccgt
    28441 gccatcatca ctaaacgagt accagtcgcg ccagggcgat tcagggttgt gacaagctcc
    28501 ccccgtgcca cgattgtgcc tgtcaaacca ggcatgggaa tcgccactgt ggttaaacac
    28561 gccgtccagc accagccgca ttcccagctg ctgcgtattg tgtcgcaaac gcagcaacgc
    28621 cccatcaccg ccaaactgcg gatcgacatg gcgataatcc tcggtatcgt atttatgtac
    28681 gctgggagct ttaaacaccg gattgagata cagcgccgtc acgccaagct ttttcagata
    28741 cggcagtttt tcgcttatcc cgtccagatc gccgccatag aacgttgatc cgcccgcctg
    28801 cgccgtgacc ggttcatccc agtcacgcaa gatgatctct tgtccggctg catgatggta
    28861 atagacatga tcctgttcag cttcacgagg aagactacgc gcaaaacgat cagggaagat
    28921 ctgataaaaa atctgatccg cagcccattg tgggccgata tccggtacat cgacggcaaa
    28981 ctgctccagt cgtgccggcg gcattcggct gaagccctgc ggtgtaaacc aacgctggcg
    29041 atcgtgccac agcagtttga aactgtaacg ccgccggggt tgtccgctgg agagatcaat
    29101 cgccgcacgc catgcggtga cgccaggctg ctgctgactg cgctgcttat gcatcgatac
    29161 tgacatttct tcgttatcgt gttctgtacg cagcataatg cgctgcggtg ggtcttcgcc
    29221 cgtcagccac agtgtaatga gcagttgatc tttgctttgt ttaacaaatg ggggcaccgg
    29281 caggtgccat gcatttaaca tcataattcc cctgtgatga aaatctgatc accttgccat
    29341 agcctaatgc aggatcactc atcatctgaa ggatatttgg gggaggagga cgggggcgga
    29401 taactttcgg aatagccgga tcaggatttc ctcctgaacc ggcttacaga acgcaagtat
    29461 tattggtttt cagccagctg acgatcgtgg cggcgtttaa acatccagcc aatcaacagc
    29521 acaacaatcc acgcgaaacc gacatacagc gagatacgcg tatccgggtg ataaccaatc
    29581 aacccgataa taaagagcag gaaaatcagc ccgccgatgg tcgttgctac cccacccggc
    29641 actttaaatt tcagcgcctt aacttcttct ggcggcaaac ggcgacggaa ggcaatttgc
    29701 gacagcagga tcataatcca cacccacacc gtggcgaagg ttgccagcga agcgatcacc
    29761 aggaagacgt tttccggcat gatgtagttc agatacaccg caaacagcag cgcggtagtc
    29821 atcaccagca ccgtaaccca cggaataccg cgacgcgacg ttttgctgaa aattttcggc
    29881 gcgctgccct gctctgccat accgtggagc atacggccta cgccaaatac atcactgtta
    29941 attgccgaca gcgaagcagt cagcacaaca aagttaagaa tgctggcggc aaaggtaatg
    30001 cccatatgct cgaacgtcag cacgaacggg ctaccggcag tgccaacctg attccacggg
    30061 tagatagaca taatgacgaa cagcgtaccg acgtagaaca ccagaatacg catcggcacg
    30121 gagttaatcg cacgcggtat cgatttctca ggatctttcg cttcaccggc ggtaatcccg
    30181 ataatttcga tcccaccgta agcaaacatc accatttgca acgacattac catgccaagc
    30241 cagccgttac tgaagaagcc gccgttgctc cacaggttat gaataccggt cggttgcccg
    30301 ccgttgccaa tcccccagat gatgatgccg aaaccggcga caatcatgat gatgatggtg
    30361 gcgactttaa agaacgagaa ccagaattcc agctcaccga agaccttcac gctcatcagg
    30421 tttacggcgc aaatgatcag caccacgctc agtacccaaa tccagtgcgg caccgtcggg
    30481 aaccagacac ccatatagat accaaaagcg gtcacatcgg cgatggcgac aataaggatt
    30541 tcaaagcagt aggtccatcc ggtaatgtaa cctgccagcg ggccgaggtt ttcctgcgca
    30601 taacgcgaga aagagctggc ggccgggtta tgtaccgaca tttcccccag cgcacgcata
    30661 atgatatacg ccgcgatacc accgataata taggccaaca acacgctcgg accggccatt
    30721 ttgatggcgt ctgccgaacc gtaaaacagc ccggtgccaa ttgctgaacc cagtgccata
    30781 aagcgtatgt ggcgggtact tagcccacgc tttagcttgt tcttactttc catcaattcc
    30841 taacccatca acacaataaa aaaccacggg gcatcgcagc cccgtggtta aaacaaatgt
    30901 tcagtgattt agtgagcgct ggaggtcacc tgacgacctg ccgcgcgatc ccagataatg
    30961 gccagaacca ccatcaccac tgttggcatt aaccacgcca gaccttgttc ggccagcggt
    31021 aaacgctgcg cccaggacgg taagatatcg ctgaatgcag atgctttgat cccgtcgaga
    31081 ataccaaaaa gcaggctgat aaacatcggc ggagcaatca cgcgggacga attatgccac
    31141 catgagcgtg taaaacttaa tacaaccagt gcgatacacg gcggataaat agcggtcagc
    31201 accggtacgg agatctggat cagctggctt aagccgaggt tagaaaccac catcgagaag
    31261 ccgccgagga taaacaccag cgtacgataa gagagcggta cgtactgggc aaagaattct
    31321 gcacaagcac aggtcaggcc aactgccgtt accaggcagg cgatgaagat taacgccgcc
    31381 agcaggaagc taccgccgcc gccaaaggtg tgctgaacgt aagcatgcag aatagcagcg
    31441 ccgtttgcag actgatcgac cagcgacgcg ctgtctgaac ccagacggaa cagcgccagg
    31501 tacagcagag tcagaccaac acccgccatc aggccagccc agacggtata acgggtcagc
    31561 agacgcgctt cggtaacgcc acgagaacgc gccgcgttaa caataacgat accaaacacc
    31621 attgcgccca gcgtatccat ggtcagatag ccgttaacga agccgttaga aaacgcagcg
    31681 ttttgataag cctcagtcgc cgtgctgata gaacccgccg gccagacaat cgcggcaaca
    31741 gacaggatga ccagcgcgat aattttcagc ggcgcaagga agttgcccac ggtatccagc
    31801 agcttgcccg gatagagcga aaccagaata acgatagcga aatagaccag gctgtagata
    31861 aacagcggca gcgcggaatc acccgtcagc ggcgcaatcc ccacttcaaa agaaacggta
    31921 gctgtacgcg gcgtagcgaa aagcggcccc accgccaggt aacaaaccgt tgccagcagt
    31981 acgccagcga ctttaccgat tggcgtgctg aggctgtcaa caccgccgcc aacttttgcc
    32041 agcgccacta ccgttaatac cggcaggcca acggcagtaa tgaggaagcc gaatgccgca
    32101 gtccagacgt gttcgcctgc ctgtaagccg accattggag ggaaaataat gttacctgcg
    32161 ccgacgaaca acgcaaatgt cataaagccc agagcgatga tatcgcgcga tcttaattga
    32221 tgggtcataa atcatactgc ctgtggatgt ggtgttgtaa cgttaaattt ttgtgccaat
    32281 ccctggggat gatacagcgg tatatttaaa caattttagc gggaaatact cccaaccagg
    32341 cgcggcgaag aatagttttt cgatttaccg cgttaatgca gtcagtatga cagcacctga
    32401 agcgcaattt aaacgcttat aacgttttaa agcaaggagg gatctaaaaa ccagaggtat
    32461 atagcgataa gaaatcacca accagtataa aacactattt ccgtgaaaaa gacatggcta
    32521 atcatgcgaa caaaaaatca ccaaccgttg taaaatctaa cgcccgtatt ttgactgcaa
    32581 agaaaataag ccagcatcgg ctggcttatg gcaataaaag atgacaaagg cggattaatc
    32641 gctgtttttg gcaattaaac gttccgggat aacaaaactg aaacgtgttc cttttcctac
    32701 tgtactctca atattcaggc gactttcgtg atgattcacc gcatgtttca cgatcgctaa
    32761 ccctaatccg ctaccgccgg tttgccggga acgcgcttta tcaacgcgat aaaaacgctc
    32821 ggtcaggcgc ggaatatgct ccggtgcaat gcccggtccg ttatcttcaa cgctaaattc
    32881 ggcaccgtgc ggcactcgct gccagcgtac ggtgatatgc gtgccttccg gcgtatgatt
    32941 cacggcgtta tagaccaggt tcgaaatcgc actgcgtagc tgatcttcgt tgccagacac
    33001 cttgaggccg ttatctatct caaaggtaaa tgtctgtttt ttctgactca gagtctgagc
    33061 ctcgcgctca acaacgcgca gcatcatcgg cacatcaacc ttttcattga gcaattgcgt
    33121 cggtgcggct tcaattttcg acagcgttag caactgcttc accagccctt ccatccgctg
    33181 ggtctgctcg cgcatggtgt gcaacgcttt ttcgcgtacc gcgccttcca gcggctgctc
    33241 atccatcatc tccaggtaac cctgtaacac ggtcaatggc gtacgtaact catggctcac
    33301 gttggcaaaa aagttacgcc gcgccccttc cagttgatgc atttgcgtga catcacgcgc
    33361 caccatcagc aactgtttgt gggtataagg catgacgcga atttccagat gccgcccggt
    33421 gttgagcacc agattgagcg ggcgagaaaa atcacgcgtt ttcaaatatt gcgtaaactc
    33481 cgggtaacgc agtaggttaa ggatgttctg cccgttatct tccggccagc gcaaaccaag
    33541 aatttgttgc gccagaccgt tacaccagaa aataccgccc tcttccgtgg tcagcaccac
    33601 cgcgtcgggc agcgactccg cgccgctacg aaagcgttta atcagattgc ccagttcacg
    33661 gcggcgtttt ttatttcgca gctgcatctg gtgtaagccg tatagtagcg gttcccagct
    33721 accacgcccc ggtggcgggg tcatactgcg atccacccac agccaccatg aaaggcgcaa
    33781 taaattccag aaatgccaga taagcagtcc tgttaccgat gccagcaaaa accagggcag
    33841 gtaaccaaaa aatgcaccca ggatgaaagc cgggaggcag caaagtagca gctccagcac
    33901 cagccttttc cacgacagcc gttccagcac gtaagatact ccagttaaga aatcataagc
    33961 cctgctctgc gtccgatgag caaggcgtta aaagcgggtt gaaaaacgat atcctgtacc
    34021 gcgcacggtc tgcaccatgc ggtcatgccc gccgggctcc agtgctttac gcaggcgacg
    34081 aatgtggaca tcgaccgtgc ggtcttccac atacacgtta gttccccaga cgtggtttaa
    34141 cagctgctcg cggctgtaca cgcgctcagg atgcgtcata aaaaagtgca gcagtttaaa
    34201 ttctgtcggc cccatctcca gcggctcttc gcccgccatc actcggtgag atgtcgggtc
    34261 gagacttaat ccctgcatct caatcacctc ttccaccgcc attggcgaaa tacggcgcat
    34321 taccgctttg attcgcgcca ccagctcctt cggcgaaaac ggcttggtga tatagtcatc
    34381 cgcgccggtt tcaaggccgc gcacgcgatc ttcttcttcc cctctggcgg tcaacatcac
    34441 cactggaata tcccgggtca tcgactcgcg cttgaggtgt ttgatgaact ggataccgga
    34501 gccgccaggt aacatccagt caaggagaat taaatccggc cagggttcat tcagttgatt
    34561 cacagcactg tcataatctt ccgcttcgac cggctgaaag ccattttgtt cgagcacgaa
    34621 gcagaccatt tcgcgaattg gagcttcatc ttctacgacc agaatacgtc tcgccatgat
    34681 ttgccctgtt gtaataaata ggttgcgatc attaatgcga cgtcattatg cgtcagattt
    34741 atgacagatt tatgaaaagc tcgtcgcaca tatcttcagg ttattgattt ccgtggcgca
    34801 gaaaaaagca aatggcacat ctgtttgggt ataatcgcgc ccatgctttt tcgccaggga
    34861 accgttatgc gcatccttca cacctcagac tggcatctcg gccagaactt ctacagtaaa
    34921 agccgcgaag ctgaacatca ggcttttctt gactggctgc tggagacagc acaaacccat
    34981 caggtggatg cgattattgt tgctggtgat gttttcgata ccggctcgcc gcccagttac
    35041 gcccgcacgt tatacaaccg ttttgttgtc aatttacagc aaactggctg tcatctggtg
    35101 gtactggcag gaaaccatga ctcggtcgcc acgctgaatg aatcgcgcga tatcatggcg
    35161 ttcctcaata ctaccgtggt cgccagcgcc ggacatgcgc cgcaaatctt gcctcgtcgc
    35221 gacgggacgc caggcgcagt gctgtgcccc attccgtttt tacgtccacg tgacattatt
    35281 accagccagg cggggcttaa cggtattgaa aaacagcagc atttactggc agcgattacc
    35341 gattattacc aacaacacta tgccgatgcc tgcaaactgc gcggcgatca gcctctgccc
    35401 atcatcgcca cgggacattt aacgaccgtg ggggccagta aaagtgacgc cgtgcgtgac
    35461 atttatattg gcacgctgga cgcgtttccg gcacaaaact ttccaccagc cgactacatc
    35521 gcgctcgggc atattcaccg cgcacagatt attggcggca tggaacatgt tcgctattgc
    35581 ggctccccca ttccactgag ttttgatgaa tgcggtaaga gtaaatatgt ccatctggtg
    35641 acattttcaa acggcaaatt agagagcgtg gaaaacctga acgtaccggt aacgcaaccc
    35701 atggcagtgc tgaaaggcga tctggcgtcg attaccgcac agctggaaca gtggcgcgat
    35761 gtatcacagg agccgcctgt ctggctggat atcgaaatca ctactgatga gtatctgcat
    35821 gatattcagc gcaaaatcca ggcattaacc gaatcattgc ctgttgaagt attgctggta
    35881 cgccggagtc gtgaacagcg cgagcgtgtg ttagccagcc aacagcgtga aaccctcagc
    35941 gaactcagcg tcgaagaggt gttcaatcgc cgtctggcac tggaagaact ggatgaatcg
    36001 cagcagcaac gtctgcagca tcttttcacc acgacgttgc ataccctcgc cggagaacac
    36061 gaagcatgaa aattctcagc ctgcgcctga aaaacctgaa ctcattaaaa ggcgaatgga
    36121 agattgattt cacccgcgag ccgttcgcca gcaacgggct gtttgccatt accggcccaa
    36181 ccggtgcggg gaaaaccacc ctgctggacg ccatttgtct ggcgctgtat catgaaactc
    36241 cgcgtctctc taacgtttca caatcgcaaa atgatctcat gacccgcgat accgctgaat
    36301 gtctggcgga ggtggagttt gaagtgaaag gtgaagcgta ccgtgcattc tggagccaga
    36361 atcgggcgcg taaccaaccc gacggtaatt tgcaggtgcc acgcgtagag ctggcgcgct
    36421 gcgccgacgg caaaattctc gccgacaaag tgaaagataa gctggaactg acagcgacgt
    36481 taaccgggct ggattacggg cgcttcaccc gttcgatgct gctttcgcag gggcaatttg
    36541 ctgccttcct gaatgccaaa cccaaagaac gcgcggaatt gctcgaggag ttaaccggca
    36601 ctgaaatcta cgggcaaatc tcggcgatgg tttttgagca gcacaaatcg gcccgcacag
    36661 agctggagaa gctgcaagcg caggccagcg gcgtcgcgtt gctcacgccg gaacaagtgc
    36721 aatcgctgac agcgagtttg caggtactta ctgacgaaga aaaacagtta cttaccgcgc
    36781 agcagcaaga acaacaatcg ctaaactggt taacgcgtca ggacgaattg cagcaagaag
    36841 ccagccgccg tcagcaggcc ttgcaacagg tgttagccga agaagaaaaa gcgcaacctc
    36901 aactggcggc gcttagtctg gcacaaccgg cacgaaatct tcgtccacac tgggaacgca
    36961 tcgcagaaca cagcgcggcg ctggcgcata ttcgccagca gattgaagaa gtaaatactc
    37021 gcttacagag cacaatggcg cttcgcgcga gcattcgcca ccacgcggcg aagcagtcag
    37081 cagaattaca gcagcagcaa caaagcctga atacctggtt acaggaacac gaccgcttcc
    37141 gtcagtggaa caacgaactg gcgggttggc gtgcgcagtt ctcccaacaa accagcgatc
    37201 gcgagcatct gcggcaatgg cagcaacagt taacccatgc tgagcaaaaa cttaatacgc
    37261 ttgcggcgat cacgttgacg ttaaccgccg atgaagttgc tagcgccctg gcgcaacatg
    37321 ccgagcaacg cccactgcgt cagcgcctgg tcgcgctgca tgggcagatt gttccccaac
    37381 aaaaacgtct ggcgcagtta caggtcgcta tccagaatgt cacgcaagaa cagacgcaac
    37441 gtaacgccgc acttaacgaa atgcgccagc gttataaaga aaagacgcag caacttgccg
    37501 atgtgaaaac catttgcgag caggaagcgc gcatcaaaac gctggaagct caacgtgcac
    37561 agttacaggc gggtcagcct tgtccacttt gtggttccac cagccacccg gcggtcgagg
    37621 cgtatcaggc gctggagcct ggtgttaatc agtctcgatt actggcgctg gaaaacgaag
    37681 ttaaaaagct cggcgaagaa ggtgcggcgc tgcgcgggca actggatgca ttaacgaagc
    37741 agcttcagcg cgatgaaaac gaagcgcaaa gcctccgaca agatgagcaa gcacttactc
    37801 aacaatggca agccgtcacg gccagcctca atatcacctt gcagccacag gacgatattc
    37861 aaccgtggct ggatgcacaa gatgagcacg aacgccagct gcggttactc agccaacgac
    37921 atgaattaca agggcagatt gccgcgcata atcagcaaat tatccagtat caacagcaaa
    37981 ttgaacaacg ccagcagcag ctcttaacgg cattggcggg ttatgcactg acattgccac
    38041 aggaagatga agaagagagc tggttggcga cacgtcagca agaagcgcag agctggcagc
    38101 aacgccagaa cgaattaacc gcgctgcaaa accgtattca gcagctgacg ccgattctgg
    38161 aaacgttgcc gcaaagtgat gatctcccgc acagcgaaga aactgtggcg ctggataact
    38221 ggcggcaggt tcatgaacaa tgtctcgcat tacacagcca gcagcagacg ttacagcaac
    38281 aggacgttct ggcggcgcaa agtctgcaaa aagcccaggc gcagtttgac accgcgctac
    38341 aggccagcgt ctttgacgat cagcaggcgt tccttgcggc gctaatggat gaacaaacac
    38401 taacgcagct ggaacagctc aagcagaatc tggaaaacca gcgccgtcag gcgcaaacgc
    38461 tggtcactca gacagcagaa acgctggcac agcatcaaca acaccgacct gacgggttgg
    38521 ctctcactgt gacggtggag cagattcagc aagagttagc gcaaactcac caaaagttgc
    38581 gtgaaaacac cacgagtcaa ggcgagattc gccagcagct gaagcaggat gcagataacc
    38641 gtcagcaaca acaaacctta atgcagcaaa ttgctcaaat gacgcagcag gttgaggact
    38701 ggggatatct gaattcgcta ataggttcca aagagggcga taaattccgc aagtttgccc
    38761 aggggctgac gctggataat ttagtccatc tcgctaatca gcaacttacc cggctgcacg
    38821 ggcgctatct gttacagcgc aaagccagcg aggcgctgga agtcgaggtt gttgatacct
    38881 ggcaggcaga tgcggtacgc gatacccgta ccctttccgg cggcgaaagt ttcctcgtta
    38941 gtctggcgct ggcgctggcg ctttcggatc tggtcagcca taaaacacgt attgactcgc
    39001 tgttccttga tgaaggtttt ggcacgctgg atagcgaaac gctggatacc gcccttgatg
    39061 cgctggatgc cctgaacgcc agtggcaaaa ccatcggtgt gattagccac gtagaagcga
    39121 tgaaagagcg tattccggtg cagatcaaag tgaaaaagat caacggcctg ggctacagca
    39181 aactggaaag tacgtttgca gtgaaataac tattcagcag gataatgaat acagaggggc
    39241 gaattatctc ttggccttgc tggtcgttat cctgcaagct atcactttat tggctacggt
    39301 gattggtagc cgttctggta gttgtgatgg tggtatgaaa aaagtcattt tatctttggc
    39361 tctgggcacg tttggtttgg ggatggccga atttggcatt atgggcgtgc tcacggagct
    39421 ggcgcataac gtaggaattt cgattcctgc tgccgggcat atgatctcgt attatgcgct
    39481 gggagtggtg gtcggtgcgc caatcatcgc actcttttcc agccgctact cactcaaaca
    39541 tatcttgttg tttctggtgg cgttgtgcgt cattggcaac gccatgttca cgctctcttc
    39601 gtcttacatg atgcttgcca ttggtcgcct ggtttccggc tttccgcatg gcgcattttt
    39661 tggcgtcggc gcgatcgtgt tatcaaaaat tatcaaaccc ggaaaagtca ccgccgccgt
    39721 ggcggggatg gtttccggga tgacagtcgc caatttgctg ggcattccgc tgggaacgta
    39781 tttaagtcag gaatttagct ggcgttacac ctttttattg atcgctgttt ttaatattgc
    39841 ggtgatggca tcggtctatt tttgggtgcc ggatattcgc gacgaggcga aaggaaagct
    39901 gcgcgaacaa tttcactttt tacgcagccc ggccccgtgg ttaattttcg ccgccacgat
    39961 gtttggcaac gcaggtgtgt ttgcctggtt cagctacgta aagccataca tgatgtttat
    40021 ttccggtttt tcggaaacgg cgatgacctt tattatgatg ttagttgggc tagggatggt
    40081 gttgggaaat atgctaagtg gcaggatttc aggacgttat tcaccactgc gcattgcagc
    40141 agtgactgac tttataattg tactggcact gctgatgctc tttttctgcg gcggcatgaa
    40201 aacaacgtcg cttatttttg cttttatttg ttgcgcgggg ttatttgccc tttcagcacc
    40261 tctgcaaata ttattactac aaaatgccaa aggcggagag ttattaggtg ccgcaggtgg
    40321 gcaaatagcg tttaaccttg gtagcgccgt cggcgcatat tgcggtggta tgatgctgac
    40381 gctggggctg gcatataatt acgtggcgct gcctgccgcc ctgctttcgt ttgctgcgat
    40441 gtcgtcattg ctgctgtatg gtcgctataa gcgccagcaa gcggcggata gtccggtgct
    40501 ggcgaaacca ctggggtaga ttgcagtcac gttgcgcgtt gcttattgcc ggatgcggcg
    40561 tgaacgcctt atccgcccta cgcggttccg gcacattttg taggcctgat aagacgcggc
    40621 aagcgtcgca tcaagcatga tgcgccaatt gcctacgttt tttactcttg tggccataac
    40681 cacgcagcgc cgcgtacgcc gctggaatca ccgtgcttcg ccttacgcac cggcgtttca
    40741 cattcgccgc cgaagacaaa ttgtttaatc aactgcccaa ccgtttgata taaacggtct
    40801 acattgctca tcccgccccc caggacaatc acatccggat cgagaatatt cacgacatgt
    40861 gccagcgatt ttgccagccg cagctcgtag cgacgcaatg ccagttccgc taccggatcg
    40921 ctttcttcaa ccaggcggat aatttcactg cctttcagcg catgtccgct caaacgacga
    40981 taatccgtcg cgaatcccgt gcccgaaata aaggtttcaa tacaaccttg tttaccgcaa
    41041 taacaaggga cttcctcgcg ataacgcagt tcgtcttcgt ccatccacgg tagcggattg
    41101 tgtccccact cacctgccgt gccattgccg ccgatatgcg cccgcccatt gaatgccacg
    41161 cccgcgccgc atcccgtgcc gataatcacg gcaaatactg tctgcgctcc cgctgccgcg
    41221 ccatctactg cttctgaaac cgccagacag ttggcgtcat ttgccagccg cacttcccgc
    41281 tgcaacctcg cgcttaagtc tttatcgaat ggctgaccgt tgagccaggt tgaattggca
    41341 ttcttaacca caccggtgta aggcgaaatt gagccaggaa tgcccatacc taccgttccg
    41401 cgctgccctg tcgcctgctc cgccatatca accaacgtgg cgatcgtttc aatagtctgc
    41461 cggtaatcat cacgcggcgt gggcagacga tggcggtaca actgctcccc tgcatcgccc
    41521 agtgcaatca cttcagtttt ggtgccgcct aaatcgatac ctatacgcac ggtactctcc
    41581 ttattttttt caatatcaat agcgtagaga cggacaaccg gattggcaat gcaaggccgc
    41641 cgacaattcg ttatcatgcc cgctaaattt aacgacaagg ccgtggaaat tatcatgctg
    41701 tggttcaaaa atttaatggt ttaccgtctt agccgcgaga tttcgctgcg tgcagaagag
    41761 atggaaaaac agctagcctc gatggcattt accccatgcg gcagccagga catggcgaag
    41821 atgggctggg ttcctccgat gggatcgcac agcgatgcgt taacgcacgt tgccaatggt
    41881 caaattgtta tctgcgcgcg caaagaagaa aaaatcctcc cgtccccggt gattaaacag
    41941 gcgctggaag cgaaaatcgc caaactggaa gcggaacagg cgcgtaagct gaagaaaacc
    42001 gaaaaagatt cgctgaaaga cgaagtgctg cactctctgt tgccgcgtgc tttcagccgt
    42061 tttagccaga caatgatgtg gatcgacacg gttaatggtt tgattatggt ggactgcgcc
    42121 agtgccaaaa aagcggaaga tacgctggca ttactgcgta aaagcctggg gtcgttaccg
    42181 gttgtaccat tgagcatgga aaacccgatt gaactgacgc tgaccgaatg ggttcgctcc
    42241 ggtagtgcgg cacagggctt ccagctgctt gatgaagccg agctgaaatc gttgctggaa
    42301 gatggcggcg tgatccgcgc gaagaaacaa gatctgacca gcgaagagat caccaatcac
    42361 attgaagccg gaaaagtggt gactaaactg gcgctcgact ggcagcagcg cattcagttt
    42421 gtgatgtgcg acgatggttc gctcaagcgt ctgaagttct gcgacgagct gcgcgatcaa
    42481 aacgaagata tcgaccgtga agatttcgcc cagcgttttg atgccgattt catcctgatg
    42541 actggtgaac tggcagcgtt aattcaaaac ctgattgaag gattaggtgg cgaagcacaa
    42601 cgttaattgc tgattttcct ttaatgccgg atgcgacgcc tgccgcgtct tatccggcgt
    42661 acgaagccac accaggcata taattattcg ctacggcgag caataatttt tagcgcagca
    42721 atattatgct ttttacgctg taacttgctc catggacgtt gtgtcattgt ttttcctcaa
    42781 gccggtgacg cgcttcactg cgaattttcc gcgttgcccc ggttttcact aaatcacgta
    42841 actgcgaaag atcgggttgt tcgacaaata agagtaatga tggatcctct tttaaccacg
    42901 ccgttttcac atcggcagac agctgtggat tattaatagc cagcgaccga tcctgaggct
    42961 cagtcaatgt cgttaacaac aacgccgggg tatgtgcgtt gcgcaatgcc gtttggcgca
    43021 aatgatcatt gcgactttgc agcaatgcac gccaccattc atcatctgat ttatctttta
    43081 tcgtagccag taccgcaaaa aatacatgga gatcttcact gatatttata tcaaccttga
    43141 tgaattatga atataccgga aatcgaaaaa atcgcagcaa aggaaatatt attatgggtt
    43201 tatgagagga tatttcagaa aaatgccctc ccgttcaagg ggaaggcgag gaattacaga
    43261 tagcgacaca gataagaggt gggttcggca acttgcagat gaaactcgct gtgaccggga
    43321 acattaaaca ccgaaccggc ttcatacact tgccagtcgg tcgcgtcagg cagtaacaca
    43381 ttcagcgcgc cactgattac cgtcatctct tccggctcag cggtgctgaa ggtgtattcg
    43441 ccttcaacca taacacccac actggcgcga ccagtgctgc tgctggaaaa gccgatcgat
    43501 ttcactttgc cggaaaagta ctcattactt tgaagcataa actggccctg tctggtagtt
    43561 gaatatctac ttcactatag gggccagagg cgctgactgt cacgcaaaat tacaccagta
    43621 attccgcagc cagccgtgca atcaatacgt tagacagcaa gacaggaaca tcgagctgtt
    43681 tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca atccagcatg atgacatcag
    43741 ccccttttgc cagtaattct ttcccggcat caatgatttt ttgttctgaa tcatgaatgg
    43801 ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg ccatttttgc gcctgaacgg
    43861 gcagcatctc ctcaaccgga acgataaccc ccacttgatg atcttcaaca atagaggaaa
    43921 ccagcggtgg caatatccgc gacggctcaa gaaagatcgt attacgcgca gtcatactac
    43981 taatgtttgc cgtactcatt aatataatga cgtcgtaacc ccgattatcg agtacttcaa
    44041 ccacaccttg caggtcacgc tccactttgc gacgcgaaac atgggccagc tggttgtcat
    44101 ttaataatgt gagaatggtg tcttcgcctg cttctggcgc gtactccgcc atcacttctt
    44161 cacgacttaa cttccccagc aggctatgat gggaaatatt atcttcgtca atgtattccg
    44221 tcaggagcgg caaaacttcc tgcataggta caatgccgat ggtcaggatc gccaacgacg
    44281 cactcattgt tcatccacct tacttttctt tcgtcgttaa ttaccgggca agtgtgaagc
    44341 accactgctg acacttactt gtgctacaaa tgacaaaaag cgtagcagca gacgtgcacg
    44401 gcataatgta aagattccaa atgattccag taatggattg ttattgttta atattctaat
    44461 tattagaaaa acatgaatta tgaaaaatgt gacgcagatc ccttgtagtg gcactcagaa
    44521 tcccttccga gcagtctgat gcgatcactc tttgtcaggg tcctcatagc gttttttcgc
    44581 atccatcgct tcctgagcgg tcggatgttc actgatcaac gagtctggtt taggatgatc
    44641 ggctctgagt tggtaccagg ttaccgtctg tcctggtttt cctttttcaa tcgtcactat
    44701 atatgcttca cgaggataag gcggtttcgt tggcatcgtt cttccttatt tcacgggatg
    44761 aacgttaagt ataggcgctc gaaaatcaac aattgatcgt ctgtgccagg gcgctacgaa
    44821 tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat gataatatgc gcaacttcgc
    44881 gatatagcgc atcgcgttct tccagcactt cctgaacttc ttcgctcagc ggttttcccg
    44941 ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag tcggttaacc aggactgata
    45001 ctggcgcaca caaataaacc acgatcccgt tattttgcat gaagtgacga ttaaattccg
    45061 tcagaataat gccgccgcct gtagcgataa cggtggatgg cgcagttacc gcttccagcg
    45121 ccgccgtttc tctggcgcga aatcccgccc actcttccct ttcgacgatc tccgcgaccg
    45181 tcatattgag ctgtgattgc aaccactgat cggtatcgac aaaccgacgg ttaagcgaat
    45241 cggcaagggc cattccgacc gttgttttac cacagccccg aggcccgatc agaaaaagag
    45301 gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg gcgaaagccg gtgtcatgag
    45361 aatagcgatc ataccatcaa actagtacaa tttcgattgt aaaggaaaaa ttccacttaa
    45421 agtgaaaatc tcaatacacc ccttactata ccaataaata atcaagaatg aagtgtaaat
    45481 aataaattac atttggccgc gactacgttg cacttccagc caccatttct ccagctctgc
    45541 agcaaaggcc tggcggtcac gttgtgaaag actatctggt ccgccggtct ggatcccact
    45601 ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc tcacgaatgg tcgctggcgt
    45661 gtaacgttcg ccgcgcggat taatcgccgc agcgcctttc tcgatggctt cagcagccaa
    45721 aggtatatct gcggtgatca ccaaatcgcc cgcttcacac tgccggacaa tttcgttatc
    45781 ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata aatcgcgatg gcggcacgcg
    45841 taaactctgg tttgctacca gtaccagcgg catctgcata cgttccgccg cgcgatacaa
    45901 aatctcttta attacattgg gacacgcgtc ggcatccacc caaattgtca taaagtcatc
    45961 ctttgttggg taatcctcta ttgtgtcgcg cttttgcctt ccggcatagt tctgtttatg
    46021 cttctgccag cgattatcaa aacaatgaat ttcacggcag gagtgaggca atggaaaaga
    46081 aaatcggttt tattggctgc ggcaatatgg gaaaagccat tctcggcggt ctgattgcca
    46141 gcggtcaggt gcttccaggg caaatctggg tatatacccc ctccccggat aaagtcgccg
    46201 ccctgcatga ccagttcggc atcaacgccg cagaatcggc gcaagaagtg gcgcaaatcg
    46261 ccgacatcat ttttgctgcc gttaaacctg gcataatgat taaagtgctt agcgaaatca
    46321 cctccagcct gaataaagac tctctggtcg tttctattgc tgcaggtgtc acgctcgacc
    46381 agcttgcccg cgcgctgggc catgaccgga aaattatccg cgccatgccg aacactcccg
    46441 cgctggttaa tgccgggatg acctccgtaa cgccaaacgc gctggtaacc cccgaagata
    46501 ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc ggaagtaatt gctgagccga
    46561 tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc ctacgtattt atgtttatcg
    46621 aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg cgcccaggcg tataaatttg
    46681 ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga aacgggagaa catccggggg
    46741 cactgaaaga tatggtctgc tcaccgggag gcaccaccat tgaagcggta cgcgtactgg
    46801 aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac gaagtgtatg gaaaaatcag
    46861 aaaaactcag caaatcctga tgatttttgc cggacgtcag gccgccactt cggtgcggtt
    46921 acgtccggct ttctttgctt tgtaaagcgc caaatctgcc gatttcaacc actcgcgata
    46981 gtgactcatt tgtgggttca gcggcgcaac ccccacacta atccgtaaag ttacctgtgg
    47041 cgtattcggc aagcgtaacg tgtttagccc ttcatgcacc cgtaacatgg cggtaatggc
    47101 gctctcagct ggcgtaccgg acatgattac cgcaaactca tcgccgccaa accgaccaat
    47161 cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc
    47221 gcccacatca tggccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag
    47281 taatgtcgca tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc
    47341 ccaatgacgt cggttataca cgcccgtcat accgtcgcgg gtactcatga cctgcaacct
    47401 gcgtttatgt tccgccagtt tagtcgccgt ctggtagctg acccagccaa acagcagagg
    47461 ataaatgacg ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac
    47521 ggtgatgccc gtcagttcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc
    47581 gacaaacaga cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatcgc
    47641 ggtggaaggc agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc
    47701 ggtttttaag ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca
    47761 ggctaaatgc ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg
    47821 cgggtgcgaa accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg
    47881 gggtagtctg acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt
    47941 taaaggaggt tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat
    48001 ttttgggaac attcagaaac aattttccca aattatagag acggggctaa aggcagaagt
    48061 atgaaatttc gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg
    48121 gctttcttga ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc
    48181 gttgcttgat tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca
    48241 ggcgcagact tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc
    48301 aacgcctgcg ccgtcgcctg ctgattacag gaggtcatac gctgttgttg cggggttaag
    48361 gttctctcgg cagcgccgac ggtggttaaa aaaaccagtc cgaaaagcaa ggtaaccagt
    48421 aatgttattt tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg
    48481 atctgccatt aagtctggtt gctaacagca aaaaaaccac ccgacagcga aaattcaccg
    48541 ccgggcgcgg ttttatttca gccccagggc ggctttcatg gtgtagaaga gatcggtctg
    48601 gtcggtcagt ccaacgacat tggcggcatg tgggccatac gccgcaatac gcagctgact
    48661 accggtatgt tcttgtgaat cctcttcgga gttcccgtaa ctcatcacca tcactgcgcc
    48721 atctttggta tttagcgcct gggtgaggcc cggcgctttg gtgtccggcg caacaatctg
    48781 gctggcgtgg gcgtgatcag cggtgactat gaccagcgtg ttgccatcct ttttagcgaa
    48841 ttccagcgcc cgttgtacgg cttcgtcgag atcgaccgtc tcgccaattt gcccacaagg
    48901 attcgcagcg tgatcctgtt tatcgattga tgcaccttca acttgcagga aaaagccttt
    48961 ctcattttta ctcaacaatt caatggcttt atcagtcatc tgcgccaggg tcggtacgct
    49021 gtcattacgt tgcggattag gcgtacaggt aactgcgggc ttgtcgatat tgccgtggta
    49081 cgttgctttc ggtccttgcc agcgcactgg catattgccg tcagcaaaca gtcctagcag
    49141 gggtttttgc tggttcgctt ccgtcaccgc attcagtgag gcagcatcgc tcaccaactg
    49201 ataaccacgc gcctgtgcct gttcacgcag cgtttttccc tgccattcac cggcggttgc
    49261 cgtttcagca aaggtttttg cgccgccgcc aagcgtaaca tcggcacgag cgttaagcag
    49321 ctgttcggta atcgatcctt ttccgccttt ttctagcgcg ttacccggac atttttcact
    49381 ggtcgcgctg ggaccgtagc atttgcgcga ggtcacatgc gccaccagcg cagcgggcgt
    49441 ggcatcctgc aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat
    49501 ttccagaatc gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt
    49561 tttgacaccg gttgaccagg cggttgctga cgcagccgaa tcggtgacgt agtccggttt
    49621 gccggttttt ttattcagcg catagtgagt gtattgcccg gtaagcggta atgcatcgat
    49681 acctttaaaa aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc
    49741 ccccatccca tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc
    49801 acgcagagcg gcggtctgat cacccgttaa gcggcgagca ccgccgggtg cagtaatatc
    49861 gccctgagca gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg
    49921 ggtaaacagt aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc
    49981 atgtacaaat acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt
    50041 tatgacagct gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta
    50101 cgtaatcgcg cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt
    50161 acagcgcccc ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa
    50221 gcattgcagt gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt
    50281 cttctttttg ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc
    50341 cttatccaaa gtatggagaa gttaacatta tgtttca
//
LOCUS       DAFNBJ010000012        50014 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000012 DAFNBJ010000000
VERSION     DAFNBJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.012"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            136..471
                     /gene="eutS"
                     /locus_tag="L2X49_000716"
     CDS             136..471
                     /gene="eutS"
                     /locus_tag="L2X49_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBN4795856.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            484..963
                     /gene="eutP"
                     /locus_tag="L2X49_000717"
     CDS             484..963
                     /gene="eutP"
                     /locus_tag="L2X49_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBN4795857.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            938..1639
                     /gene="eutQ"
                     /locus_tag="L2X49_000718"
     CDS             938..1639
                     /gene="eutQ"
                     /locus_tag="L2X49_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HBN4795858.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1636..2439
                     /gene="eutT"
                     /locus_tag="L2X49_000719"
     CDS             1636..2439
                     /gene="eutT"
                     /locus_tag="L2X49_000719"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HBN4795859.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2436..3452
                     /gene="pta"
                     /locus_tag="L2X49_000720"
                     /pseudo
     CDS             2436..3452
                     /gene="pta"
                     /locus_tag="L2X49_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
     gene            3491..3784
                     /gene="eutM"
                     /locus_tag="L2X49_000721"
     CDS             3491..3784
                     /gene="eutM"
                     /locus_tag="L2X49_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBN4795860.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3891..4178
                     /gene="eutN"
                     /locus_tag="L2X49_000722"
     CDS             3891..4178
                     /gene="eutN"
                     /locus_tag="L2X49_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HBN4795861.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4190..5593
                     /locus_tag="L2X49_000723"
     CDS             4190..5593
                     /locus_tag="L2X49_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HBN4795862.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5604..6440
                     /gene="eutJ"
                     /locus_tag="L2X49_000724"
     CDS             5604..6440
                     /gene="eutJ"
                     /locus_tag="L2X49_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HBN4795863.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6430..7617
                     /gene="eutG"
                     /locus_tag="L2X49_000725"
     CDS             6430..7617
                     /gene="eutG"
                     /locus_tag="L2X49_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HBN4795864.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSEISVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7734..8960
                     /gene="eutH"
                     /locus_tag="L2X49_000726"
     CDS             7734..8960
                     /gene="eutH"
                     /locus_tag="L2X49_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBN4795865.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            8957..10360
                     /gene="eutA"
                     /locus_tag="L2X49_000727"
     CDS             8957..10360
                     /gene="eutA"
                     /locus_tag="L2X49_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBN4795866.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10372..11733
                     /gene="eutB"
                     /locus_tag="L2X49_000728"
     CDS             10372..11733
                     /gene="eutB"
                     /locus_tag="L2X49_000728"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HBN4795867.1"
                     /translation="MKLKTTLFSNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11754..12641
                     /gene="eutC"
                     /locus_tag="L2X49_000729"
     CDS             11754..12641
                     /gene="eutC"
                     /locus_tag="L2X49_000729"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HBN4795868.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12651..13310
                     /gene="eutL"
                     /locus_tag="L2X49_000730"
     CDS             12651..13310
                     /gene="eutL"
                     /locus_tag="L2X49_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBN4795869.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13323..13823
                     /gene="eutK"
                     /locus_tag="L2X49_000731"
     CDS             13323..13823
                     /gene="eutK"
                     /locus_tag="L2X49_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBN4795870.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13869..14921
                     /gene="eutR"
                     /locus_tag="L2X49_000732"
     CDS             13869..14921
                     /gene="eutR"
                     /locus_tag="L2X49_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HBN4795871.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(14927..15826)
                     /gene="hemF"
                     /locus_tag="L2X49_000733"
     CDS             complement(14927..15826)
                     /gene="hemF"
                     /locus_tag="L2X49_000733"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBN4795872.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15830..16699)
                     /gene="amiA"
                     /locus_tag="L2X49_000734"
     CDS             complement(15830..16699)
                     /gene="amiA"
                     /locus_tag="L2X49_000734"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBN4795873.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            16913..17338
                     /locus_tag="L2X49_000735"
     CDS             16913..17338
                     /locus_tag="L2X49_000735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBN4795874.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17325..17774
                     /locus_tag="L2X49_000736"
     CDS             17325..17774
                     /locus_tag="L2X49_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBN4795875.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            17835..18410
                     /locus_tag="L2X49_000737"
     CDS             17835..18410
                     /locus_tag="L2X49_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBN4795876.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18506..19405
                     /gene="yfeX"
                     /locus_tag="L2X49_000738"
     CDS             18506..19405
                     /gene="yfeX"
                     /locus_tag="L2X49_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBN4795877.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19463..20767)
                     /gene="pbp4b"
                     /locus_tag="L2X49_000739"
     CDS             complement(19463..20767)
                     /gene="pbp4b"
                     /locus_tag="L2X49_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HBN4795878.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20772..22196)
                     /gene="murP"
                     /locus_tag="L2X49_000740"
     CDS             complement(20772..22196)
                     /gene="murP"
                     /locus_tag="L2X49_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBN4795879.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22200..23096)
                     /gene="murQ"
                     /locus_tag="L2X49_000741"
     CDS             complement(22200..23096)
                     /gene="murQ"
                     /locus_tag="L2X49_000741"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBN4795880.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23260..24117
                     /gene="murR"
                     /locus_tag="L2X49_000742"
     CDS             23260..24117
                     /gene="murR"
                     /locus_tag="L2X49_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBN4795881.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24246..25037
                     /gene="ucpA"
                     /locus_tag="L2X49_000743"
     CDS             24246..25037
                     /gene="ucpA"
                     /locus_tag="L2X49_000743"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBN4795882.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25208..26224
                     /gene="cysP"
                     /locus_tag="L2X49_000744"
     CDS             25208..26224
                     /gene="cysP"
                     /locus_tag="L2X49_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBN4795883.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26224..27057
                     /gene="cysT"
                     /locus_tag="L2X49_000745"
     CDS             26224..27057
                     /gene="cysT"
                     /locus_tag="L2X49_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBN4795884.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSINTLQSRFGRRVVG
                     H"
     gene            27057..27932
                     /gene="cysW"
                     /locus_tag="L2X49_000746"
     CDS             27057..27932
                     /gene="cysW"
                     /locus_tag="L2X49_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBN4795885.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            27922..29019
                     /gene="cysA"
                     /locus_tag="L2X49_000747"
     CDS             27922..29019
                     /gene="cysA"
                     /locus_tag="L2X49_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBN4795886.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVSLLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29153..30064
                     /gene="cysM"
                     /locus_tag="L2X49_000748"
     CDS             29153..30064
                     /gene="cysM"
                     /locus_tag="L2X49_000748"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBN4795887.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30067..30435)
                     /locus_tag="L2X49_000749"
     CDS             complement(30067..30435)
                     /locus_tag="L2X49_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBN4795888.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30540..31391
                     /gene="pdxK"
                     /locus_tag="L2X49_000750"
                     /gene_synonym="thiJ"
     CDS             30540..31391
                     /gene="pdxK"
                     /locus_tag="L2X49_000750"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBN4795889.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31433..31942)
                     /gene="crr"
                     /locus_tag="L2X49_000751"
     CDS             complement(31433..31942)
                     /gene="crr"
                     /locus_tag="L2X49_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBN4795890.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(31983..33710)
                     /gene="ptsI"
                     /locus_tag="L2X49_000752"
     CDS             complement(31983..33710)
                     /gene="ptsI"
                     /locus_tag="L2X49_000752"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBN4795891.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33755..34012)
                     /gene="ptsH"
                     /locus_tag="L2X49_000753"
     CDS             complement(33755..34012)
                     /gene="ptsH"
                     /locus_tag="L2X49_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBN4795892.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34396..35367)
                     /gene="cysK"
                     /locus_tag="L2X49_000754"
     CDS             complement(34396..35367)
                     /gene="cysK"
                     /locus_tag="L2X49_000754"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBN4795893.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35552..36313)
                     /gene="cysZ"
                     /locus_tag="L2X49_000755"
     CDS             complement(35552..36313)
                     /gene="cysZ"
                     /locus_tag="L2X49_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBN4795894.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36543..37529
                     /gene="zipA"
                     /locus_tag="L2X49_000756"
     CDS             36543..37529
                     /gene="zipA"
                     /locus_tag="L2X49_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBN4795895.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37600..39615
                     /gene="ligA"
                     /locus_tag="L2X49_000757"
     CDS             37600..39615
                     /gene="ligA"
                     /locus_tag="L2X49_000757"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBN4795896.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39617..39835
                     /locus_tag="L2X49_000758"
     CDS             39617..39835
                     /locus_tag="L2X49_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBN4795897.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(39832..40830)
                     /locus_tag="L2X49_000759"
     CDS             complement(39832..40830)
                     /locus_tag="L2X49_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBN4795898.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            40920..41846
                     /locus_tag="L2X49_000760"
     CDS             40920..41846
                     /locus_tag="L2X49_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4795899.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(41885..42649)
                     /locus_tag="L2X49_000761"
     CDS             complement(41885..42649)
                     /locus_tag="L2X49_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4795900.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            42898..43731
                     /gene="xapA"
                     /locus_tag="L2X49_000762"
     CDS             42898..43731
                     /gene="xapA"
                     /locus_tag="L2X49_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HBN4795901.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            43791..45047
                     /gene="xapB"
                     /locus_tag="L2X49_000763"
     CDS             43791..45047
                     /gene="xapB"
                     /locus_tag="L2X49_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HBN4795902.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKAVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            45100..45258
                     /locus_tag="L2X49_000764"
     CDS             45100..45258
                     /locus_tag="L2X49_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HBN4795903.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            45299..46183
                     /gene="xapR"
                     /locus_tag="L2X49_000765"
     CDS             45299..46183
                     /gene="xapR"
                     /locus_tag="L2X49_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HBN4795904.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(46292..46367)
                     /locus_tag="L2X49_000766"
     tRNA            complement(46292..46367)
                     /locus_tag="L2X49_000766"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46332..46334),aa:Lys,seq:ttt)
     gene            complement(46372..46447)
                     /locus_tag="L2X49_000767"
     tRNA            complement(46372..46447)
                     /locus_tag="L2X49_000767"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46412..46414),aa:Val,seq:tac)
     gene            complement(46494..46569)
                     /locus_tag="L2X49_000768"
     tRNA            complement(46494..46569)
                     /locus_tag="L2X49_000768"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46534..46536),aa:Val,seq:tac)
     gene            complement(46614..46689)
                     /locus_tag="L2X49_000769"
     tRNA            complement(46614..46689)
                     /locus_tag="L2X49_000769"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46654..46656),aa:Val,seq:tac)
     gene            46948..48363
                     /gene="gltX"
                     /locus_tag="L2X49_000770"
     CDS             46948..48363
                     /gene="gltX"
                     /locus_tag="L2X49_000770"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBN4795905.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(48415..48699)
                     /locus_tag="L2X49_000771"
                     /pseudo
     CDS             complement(48415..48699)
                     /locus_tag="L2X49_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
     gene            48766..49770
                     /locus_tag="L2X49_000772"
     CDS             48766..49770
                     /locus_tag="L2X49_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HBN4795906.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
BASE COUNT        11750 a        12600 c        13898 g        11766 t
ORIGIN      
        1 agtttatgca ccacagcgaa tatctctcca ttcttagtga tctacctcac cttttaaacg
       61 cgcttgccga attttgttat ttactctgac gaaaaattgt cacgatacac gaaagttttt
      121 cacaggcggc gactcatgga taaagaacgc atcattcagg aatttgtgcc cggcaaacag
      181 gtcacactgg cgcatctcat cgcgcatccc ggtgaggaac tggcgaagaa aatcggcgtt
      241 ccggatgcgg gcgcaattgg cattatgacg ctaactcccg gcgaaacggc gatgatcgcc
      301 ggtgatttag cgctgaaagc cgctgatgtg catatcggtt ttctcgaccg cttcagcggt
      361 gcattggtga tttatggctc ggtgggcgca gtagaagaag cgttatcaca aaccgtcagc
      421 ggtttaggtc gcttattaaa ttatacgctt tgtgaaatga ccaaaagtta atacgaggca
      481 ttcatgaaac gaattgcgtt tgtgggtagc gtcggggcag gaaaaacaac gttatttaat
      541 gcgttgcagg ggaattatac cctcgccaga aaaacacagg ccgtggaatt taatgataag
      601 ggcgatattg atacgccggg tgaatatttt agccatcccc gctggtatca cgccttaatt
      661 accacgctac aggatgtaga catgctgatc tatgtccatg gcgcaaatga cccggaaagt
      721 cggttgcctg ccgggttgct ggatattggc gtcagcaaac ggcaaatcgc cgtcatcagt
      781 aaaacggaca tgccagatgc tgacgtcgcc gcaacgcgaa agttattgct tgagacgggt
      841 ttcgaagagc cgattttcga gctaaacagc cacgacccgc aaagcgtgca gcaactggtg
      901 gattatctgg catctctcac caaacaggag gaagcaggtg aaaaaactca tcacagcgaa
      961 tgatattcgt gaagcacacg cgcgcggcga acaggcaatg tccgtggttc tgcgcgccag
     1021 cattattacc ccggaagccc gtgaagtggc ggatctactg ggttttacca tcaccgaatg
     1081 tgatgaatcg atcccggtaa cggcgtccgt gcccgccagc gtgccagccg acaaaaccga
     1141 aagccagcgc atccgcgaaa ccatcattgc ccagctgccg gaaggccagt ttaccgaaag
     1201 cctggtcgca cagctgatgg aaaaagtgat gaaggaaaaa cagtcgctgg agcagggcgc
     1261 aatgcagccg agcttcaaat cggtgaccgg caaaggcggc atcaaagtga ttgatggcag
     1321 cagcgtcaag tttggtcgtt tcgacggcgc agagccgcat tgcgtgggct tgactgatct
     1381 tgtgaccgga gacgacggca gcagcatggc cgccggtttt atgcagtggg agaacgcatt
     1441 cttcccgtgg accctgaact acgacgaaat cgacatggtg ctggaagggg agctacatgt
     1501 tcgccacgaa ggcgaaacca tgatcgccaa agcaggcgac gtgatgttta tcccgaaagg
     1561 ctccagcatc gaatttggta cgacatccag cgtgaaattc ctgtatgtcg cctggccggc
     1621 taactggcaa tccctatgaa agatttcatc accgaagcat ggctaagagc gaaccatacg
     1681 ctcagcgaag gcgcagagat ccatctccct gcggacagtc gcctgacgcc ctctgcccgg
     1741 gagttgctgg aaagccgcca tctgcgcatc aagtttattg acgagcaggg ccgcctgttt
     1801 gttgacgatg aacagcagca gccgcagccc gttcatgggc tgaccagtag cgatgaacat
     1861 ccgcaggcgt gctgcgaact gtgccgccag ccggtggcaa aaaagccgga tacgctgact
     1921 cacctgtcgg cggaaaaaat ggtcgccaaa agcgatccgc gtctgggttt tcgcgcggtt
     1981 ctcgacagca ccattgcgct ggcagtgtgg ctgcaaattg aactggcgga accgtggcag
     2041 ccgtggctgg cggatatccg ttcgcgtctt ggcaacatta tgcgcgccga tgcgctgggg
     2101 gaaccgctgg gctgccaggc gattgtcggg ctttctgacg aagatctgca tcggctttct
     2161 catcagccgc tgcgctatct cgaccacgat catctggtgc cggaagccag ccacgggcgc
     2221 gatgctgcgt tgctcaatct gctacgcacc aaagtgcggg aaaccgaaac cgtagcggcg
     2281 caggtcttta tcacccgcag ctttgaagta ttacgcccgg acattttgca ggcgctgaac
     2341 cgcctttcga gcacggtcta cgtgatgatg atcctgagcg taacgaagca gccgctaacg
     2401 gtgaagcaga tccaacaacg actgggggaa acgcaatgat tattgaacgt tgtcgtgaac
     2461 tggcgttgcg agcgcccgcc agagtggttt ttccggatgc gttagaccaa cgtgtgctga
     2521 aagccgcgca atatttacat caacaaggtc tggcaacgcc cattctggtc gccaatccgt
     2581 ttgaacttcg tcagtttgcg ctcagtcacg gcgtggcgat ggacgggcta taggtgattg
     2641 atccgcatgg caacctcgca atgcgggaag aatttgctca tcgctggctg gcccgcgcgg
     2701 gcgaaaaaac gccgccggat gcgctggaaa aacttaccga ccctctgatg ttcgccgcag
     2761 caatggtcag cgccggtaaa gcggatgtct gtatcgcggg caacctctct tccacggcaa
     2821 atgtgctgcg tgccggatta cgcattatcg gcttgcagcc aggctgtaaa acgctctctt
     2881 ccattttcct gatgttgcca cagtacagcg gcccggcgtt gggctttgcc gattgcagcg
     2941 tggtgccaca gccgacggcg gcgcagctgg cggatatcgc gcttgccagt gccgaaacct
     3001 ggcgcgccat caccggagaa gagccgcgcg tggcgatgct gtcgttttcc agtaacggta
     3061 gcgcccgtca cccctgcgtt gccaacgtcc agcaggcgac agaaatcgtc cgtgagcgcg
     3121 caccaaagct ggtggttgat ggcgagttac agtttgacgc cgccttcgtg ccggaagtgg
     3181 cggcgcaaaa agcgcctgcc agcccgctac agggcaaggc caatgtgatg gtttttccgt
     3241 cgctggaagc cggaaatatt ggctacaaaa tcgcacaacg actcggcgga tatcgtgccg
     3301 tcgggccatt gatacaagga cttgccgcgc cgatgcacga tctctctcgt ggttgtagcg
     3361 tgcaggaaat tatcgagctg gcgctggtgg cagctgtgcc gcgtcagaca aaagtgaacc
     3421 gcgaaagcag tttacaaaca ctggttgaat gaacggtccc gttctggacc ctgataaaga
     3481 ggaaagcaca atggaagcat taggaatgat cgaaacccgg ggcctggttg cactgattga
     3541 ggcttctgac gcgatggtga aagctgcgcg cgtcaagctg gttggtgtta agcagattgg
     3601 cggcggcctg tgtactgcca tggttcgcgg tgatgttgca gcctgtaaag cggcgaccga
     3661 tgccggtgcc gcagcggcac agcgtattgg cgagctggta tccgtacacg ttatcccgcg
     3721 tccgcacggt gacctggaag aagtcttccc gatcggcctg aaaggcgata gcagcaacct
     3781 gtaatcactc tgtgtgacgt cgattcgggc gtcacgctct ctcctttttc attttacctt
     3841 ctgcggaggg tagggggagc ttttacctga aatacgccac ggaggcgggt atgaaactgg
     3901 cagtcgtcac tggacaaatt gtttgtaccg tacgccatca cggactggcg catgacaaat
     3961 tgctgatggt ggaaatgatt gatccacaag gtaatcctga cgggcaatgc gccgtcgcca
     4021 tcgacaatat tggcgcggga accggggagt gggtgttact ggtgagtggc agttccgccc
     4081 gccaggcgca taaaagcgaa acgtcaccgg tcgatctgtg cgtgattggc attgtcgatg
     4141 aggtggtgtc tggcggtcag gtaattttcc acaaataagg cagaacatca tgaatcaaca
     4201 ggatattgaa caggtggtga aagcggtact gctgaaaatg caaagcagtg acacgccgtc
     4261 cgccgccgtt catgagatgg gcgttttcgc gtccctggat gacgccgttg cggcagccaa
     4321 agtcgcccag caagggttaa aaagcgtggc aatgcgccag ttagccattg ctgccattcg
     4381 tgaggcaggc gaaaaacacg ccagagattt agcggaactt gccgtcagtg aaaccggcat
     4441 gggccgcgtt gaagataaat ttgccaaaaa cgtcgctcag gcgcgcggca cgccaggcgt
     4501 tgagtgtctc tctccgcagg tgctgaccgg cgacaacggc ctgactttaa ttgaaaacgc
     4561 gccctggggc gtggtggctt cggtaactcc gtccactaac ccggcggcaa ccgtaattaa
     4621 taacgccatc agcctgattg ccgcgggcaa cagcgtcatt tttgccccgc atccggcggc
     4681 gaaaaaagtc tcccagcggg cgattacgct gctcaaccag gcgattgttg ccgcaggtgg
     4741 gccggaaaac ttactggtta ctgtggcaaa tccggatatc gaaaccgcgc aacgcttgtt
     4801 caagtttccg ggtatcggcc tgctggtggt aaccggcggc gaagcggtag tagaagcggc
     4861 gcgtaaacac accaataaac gtctgattgc cgcaggcgct ggcaacccgc cggtagtggt
     4921 ggatgaaacc gctgacctcg cccgtgctgc tcagtccatc gtcaaaggcg cgtctttcga
     4981 taacaacatc atttgtgccg acgaaaaggt actgattgtt gttgatagcg tagccgatga
     5041 actgatgcgt ctgatggaag gccagcacgc ggtgaaactg accgcagaac aggcgcagca
     5101 gctgcaaccg gtgttgctga aaaatatcga cgagcgcgga aaaggcaccg tcagccgtga
     5161 ctgggttggt cgcgatgcgg cgaaaatcgc ggcggcaatc ggcctgaacg tgccgcaaga
     5221 aacgcgtttg ctgtttgtgg aaaccactgc agaacatccg tttgccgtga ccgaactgat
     5281 gatgccggtg ctgcccgtcg tgcgcgtcgc caacgtggcg gatgccattg cgctggcggt
     5341 gaaactggaa ggcggttgcc accacacggc ggcaatgcac tcgcgcaaca tcgaaaacat
     5401 gaaccagatg gcgaatgcta ttgataccag cattttcgtt aagaacggac cgtgcattgc
     5461 cgggctgggg ctgggcgggg aaggctggac caccatgacc atcaccacgc caaccggtga
     5521 aggggtaacc agcgcgcgta cgtttgtccg tctgcgtcgc tgtgtattag tcgatgcgtt
     5581 tcgcattgtt taaggagtaa cagatggcgc acgacgaaca atggctcacc ccacgtctgc
     5641 aaacggcggc aacgctgtgt aaccagacgc ccgccgcgac ggaatctccg ctgtggctgg
     5701 gggtggatct gggcacctgc gatgtggtgt cgatggttgt tgaccgcgac ggtcagccgg
     5761 tggcggtatg cctcgactgg gccgatgtgg tccgcgacgg catcgtctgg gatttcttcg
     5821 gtgcggtcac cattgttcgt cgccatctcg acactcttga gcagcaattc ggtcttcgtt
     5881 ttagccatgc ggcgacttca tttcctccag gtacggaccc gcgtatttcc atcaacgtgc
     5941 tggaatctgc cgggctggaa gtgagccatg tgctggatga gccaaccgca gtcgcggatc
     6001 tgctgcaact ggacaacgcc ggtgtagtgg atatcggcgg cggcactacc ggcattgcca
     6061 tcgtgaaaaa gggcaaggtg acgtactcgg cggatgaagc caccggcgga catcacatct
     6121 ctctgactct cgccggaaat cgtcgtattt cgctggaaga ggcagagcag tacaagcgcg
     6181 gtcatggtga cgagatttgg cccgcggtga aaccggtgta cgaaaaaatg gcggacattg
     6241 tcgcccggca tattgaaggg cagggcatta ctgatttatg gctggcgggc ggctcttgta
     6301 tgcaaccggg cgtggcggaa ctgtttcgca aacaattccc ggcgttacag gtgcatttac
     6361 cgcagcacag tttgtttatg accccgctgg cgatcgccag tagtgggcga gagaaagcgg
     6421 aggggctata tgcaaaatga attgcagacc gcgctctttc aggcgttcga taccctgaat
     6481 ctgcaacggg taaaaacatt tagcgttcca ccggtgacgc tttgcggtcc gggctcggtg
     6541 agcagttgcg gacagcaagc gcaaacgcgt gggctgaaac atctgttcgt gatggcagac
     6601 agctttttgc atcaggcagg gatgaccgcc gggctgacgc gtagcctggc cgttaaaggt
     6661 atcgccatga cgctctggcc atgtccggtg ggcgaaccgt gcatcaccga cgtgtgtgca
     6721 gccgtggcgc agttgcgtga gtcaggctgt gatggggtga tcgcatttgg cggcggctcg
     6781 gtgctggatg cggcgaaagc cgtggcgttg ctggtgacga acccggatag cacgctggca
     6841 gagatgtcag aaatcagcgt tctgcaaccg cgcttgccgc tgattgccat tccaacgacc
     6901 gccggaaccg gctctgaaac caccaatgta acggtgatta tcgacgcggt gagcgggcgc
     6961 aagcaggtgt tagcccatgc ctcgctgatg ccggatgtgg cgatcctcga cgccgcattg
     7021 accgaaggtg tgccgtcgca tgtcacggcg atgaccggca ttgatgcgtt aacccatgcc
     7081 attgaagcat acagcgccct gaacgctaca ccgtttaccg acagtctggc gattggtgcc
     7141 attgcgatga ttggcaaatc gctgccgaaa gcggtgggct acggtcacga ccttgccgcg
     7201 cgcgagagca tgttgctggc ttcatgtatg gcgggaatgg cgttttccag tgcgggtctt
     7261 gggttgtgcc acgcgatggc gcatcagccg ggcgcggcgc tgcatattcc acacggtctg
     7321 gcgaacgcca tgttgctgcc aacggtgatg gaatttaacc ggatggtttg tcgcgaacgg
     7381 tttagtcaga ttggtcgggc actgcgaact aaaaaatccg acgatcgtga cgctattaac
     7441 gcggtaagtg agctgattgc ggaagttggg attggtaaac gactgggcga tgttggcgcg
     7501 acatctgcgc attacggtgc atgggcgcag gccgcgcagg aagatatttg tctgcgcagc
     7561 aacccgcgta ccgccagcct ggagcagatt gtcggcctgt acgcagcggc gcaataaatg
     7621 ccggatgcga cgcttgccgc gtctgatccg gccttcggga ttgcacatgt agggcggata
     7681 aggcgtttac gccgcatccg ccaataaata acaactaaca gggagtaaag gcgatgggaa
     7741 ttaacgaaat catcatgtac atcatgatgt tctttatgct gatagctgcc gtagacagga
     7801 tcctgtcgca gttcggcggt tctgctcgtt tcctcggtaa gttcggtaaa agtatcgaag
     7861 gatcaggcgg tcagttcgaa gaaggcttta tggcaatggg cgctctgggt ctggcgatgg
     7921 tcggtatgac cgcgctggca ccggtactgg ctcacgtact cgggccggta attatcccgg
     7981 tttacgaaat gctcggcgca aacccgtcaa tgttcgccgg aacgctgctg gcgtgcgata
     8041 tgggcggttt cttcctcgcc aaagagctgg cgggcggcga cgtagcagcg tggctatact
     8101 ctgggttaat tcttgggtcg atgatggggc caacgattgt gttttccatt ccggtggcgc
     8161 tcggcattat cgaaccttct gaccgtcgtt atctggcgct cggcgtgctg gcgggcattg
     8221 tgaccattcc gattggctgt attgctggtg gtctggttgc tatgtactcc ggtgtgcaga
     8281 tcaacggtca gccggtggaa ttcactttcg ccctgatcct gatgaacatg atcccggtga
     8341 tcattgtcgc gattctggtg gcgctggggc tgaaattcat cccggaaaaa atgatcaacg
     8401 gcttccagat cttcgccaaa ttcctcgttg cattgatcac cctcggtctt gccgctgcgg
     8461 tagtgaaatt cctccttggc tgggaactga tccccggact ggatcctatc tttatggccc
     8521 ctggcgataa acccggtgaa gtgatgcgcg ccattgaagt tatcggttct atctcctgcg
     8581 tgctgttagg ggcgtatcca atggtgctgc tgctgactcg ctggtttgaa aaaccgctga
     8641 tgagcgtcgg taaagtgctg aatatgaaca acatcgcggc agccggcatg gtggcaacgc
     8701 ttgccaacaa catcccgatg ttcggcatga tgaagcagat ggatacccgc ggcaaagtca
     8761 tcaactgcgc cttcgccgtt tccgctgctt tcgccctggg cgaccactta ggcttcgccg
     8821 ctgccaacat gaacgccatg atcttcccga tgattgtcgg caagttgatc ggcggcgtaa
     8881 cggcgattgg cgtggcgatg atgctggtgc caaaagaaga cgcgaccgcg actaaaaccg
     8941 aagcggaggc acaatcgtga acactcgcca gctattgagc gtcggtatcg atatcggcac
     9001 caccaccacc caggtgattt tctcccacct ggaactggtt aaccgtgcgg cggtgtcgca
     9061 ggtgccgcgc tacgaattca ttaaacgcga aattagctgg caaagcccgg tattctttac
     9121 ccctgtcgat aaacagggtg gtttaaaaga agcggaactg aaatcgctga ttttagagca
     9181 atatcaggct gcgggcattg cgccggaaag cgttgattct ggtgccatca tcatcaccgg
     9241 tgaaagcgcg aaaacccgca atgctcgccc ggcggtgatg gcgctctctc agtcgctggg
     9301 cgattttgtc gttgccagcg ccgggccgca cctcgaatcc gtgatcgccg gtcacggagc
     9361 cggggcacaa accctttctg aacaacggct gtgtcgggta ctgaatatcg acatcggcgg
     9421 tggcaccgcg aactacgccc tgttcgatgc cggaaaaatc agcggcactg cctgtctcaa
     9481 cgtcggtggt cgcctgctgg aaaccgacag ccaggggcgc gtggtttacg ctcataaacc
     9541 ggggcagatg attgtggatg agtgcttcgg tgcaggcact gacgcccgtt cgctgaccgg
     9601 cgcgcagctg gtgcaggtta cccggcggat ggcggcgctg attgtcgaag tgattgacgg
     9661 aacgctttcg ccgctcgcgc aggcattgat gcaaaccggt ttgctgcccg caggtgttac
     9721 gcccgaaatc attacgcttt ctggaggcgt gggcgaatgt tatcgccacc agcccgccga
     9781 cccgttctgt tttgccgata ttggcccact tctggcaacg gcgctgcatg accatccgcg
     9841 cctgcgtgag atgaatgtgc agtttccggc gcaaaccgta cgcgccacgg tgattggcgc
     9901 gggtgcacat accctttcgc tctctggcag cacaatctgg ctggagggcg tacaactgcc
     9961 gctgcgcaat ttgccggtgg cgatcccgat tgatgaaacg gatctggtga gtgcctggca
    10021 acaggcgctg cttcagctgg atcttgatcc caaaactgac gcgtacgtgc tggcgcttcc
    10081 cgcctcgctg cctgtgcgtt acgccgcggt actgacggtc atcaacgcgc tggtcgattt
    10141 cgtcgcgcgt tttccgaatc cgcatcccct gctggtggtg gccgggcagg actttggtaa
    10201 agctctgggc atgttgttgc gcccacagct acaacaactc ccgttggcag tcattgacga
    10261 agtgattgtc cgcgcggggg actatatcga cattggtacg cctctttttg gcggatcggt
    10321 tgtgccggtg acggtgaaat cactcgcatt tccttcctga gggaacgact tatgaaacta
    10381 aagaccacat tgttcagcaa tgtatatcag tttaaggatg taaaagaggt gctggctaaa
    10441 gccaacgaac tgcgttcggg ggatgtgctg gcaggcgttg cagcggcaag ttcacaggag
    10501 cgcgtggcgg caaagcaggt gttgtcggaa atgaccgtag cggacatccg caataatccg
    10561 gtgattgcct atgaagatga ctgcgtgacg cggctgattc aggacgatgt taacgaaacg
    10621 gcctacaacc agattaaaaa ctggagcatc agcgaactgc gtgagtatgt gctgagcgat
    10681 gaaaccagcg tggacgacat tgcctttacc cgcaaagggc tgacctcgga agtggtcgcg
    10741 gcggtagcga agatttgctc caacgcggac ctgatctacg gcgcgaagaa aatgccggta
    10801 atcaaaaagg ccaataccac cattggtatt ccgggcacct tcagcgcccg tttgcagcca
    10861 aatgacaccc gtgacgacgt gcaaagtatc gccgcgcaaa tctacgaagg gctttccttc
    10921 ggggtgggcg atgcggtgat cggcgttaac ccggtgactg acgacgtgga aaacttaagc
    10981 cgcgtgttgg acaccattta cggcgtgatc gacaaattca acatcccaac tcagggctgc
    11041 gtactggcgc acgtcaccac ccagatcgaa gcgattcgtc gcggcgcgcc tggcggactg
    11101 attttccaga gtatctgtgg cagcgaaaaa gggctgaaag agtttggcgt ggaactggcg
    11161 atgctcgacg aagcgcgcgc agtgggtgca gagttcaacc gtatcgccgg ggaaaactgc
    11221 ctctacttcg aaaccggaca aggctctgcg ctatccgctg gcgctaactt cggcgctgac
    11281 caggtgacga tggaagcacg taactatggg ctggcgcgtc attacgatcc gtttatcgtc
    11341 aacaccgtgg tcggctttat tgggccggag tatctctaca acgaccgcca gattatccgt
    11401 gctggcttag aagatcactt tatgggcaag ctgagcggca tctctatggg ctgtgactgc
    11461 tgttacacca accacgctga cgctgaccag aacctcaacg aaaacctgat gatcctgctc
    11521 gccaccgcag gctgcaacta catcatgggg atgccgctgg gtgatgacat catgctcaac
    11581 taccagacca ccgcattcca cgataccgcc actgtgcgtc agttactcaa cctgcgtccg
    11641 tcaccggagt ttgaacgctg gctggaaagc atgggcatta tggcaaacgg tcgcctgacc
    11701 aaacgggcgg gcgatccgtc actgttcttc tgatgacgcg gagataacac atcatggatc
    11761 aaaaacagat tgaagaaatt gtacgcagcg tgatggcgtc aatgggacaa acggcccccg
    11821 cgccgtcaga agcaaagtgc gccaccacca actgtgcggc accggtgacc tcggaaagct
    11881 gtgcgctgga tttaggttcc gctgaagcaa aagcgtggat tggtgttgaa aacccgcatc
    11941 gcgcagacgt attaacagaa ctgcgccgca gcaccgtggc ccgcgtttgt accggtcgtg
    12001 ccggtccgcg tccacgtacc caggcgctgc tgcgtttcct ggccgatcac tctcgttcga
    12061 aagataccgt actgaaagaa gtgccggaag agtgggtgaa agcgcagggc ttgctggaag
    12121 tacgctctga aattagcgac aaaaatctct acctgactcg cccggatatg ggccgccgcc
    12181 tgtgtgcaga agctgttgaa gcgctgaaag cacagtgtgt tgctaatccg gacgtacagg
    12241 tcgttatttc tgatggcctg tcaactgatg ccatcaccgt gaactacgaa gagatcctgc
    12301 cgccgctgat ggcgggcctg aaacaggccg ggctgaaagt cggcacgcca ttctttgttc
    12361 gttatggtcg cgtgaagatt gaagatcaga tcggtgagat cctcggcgcg aaagtggtga
    12421 tcctgctggt gggggaacgt ccggggttag ggcagtcaga aagcctctcc tgctacgccg
    12481 tttactcgcc gcgtatggcg accaccgttg aggccgatcg cacctgtatc tctaacattc
    12541 accagggcgg cacgccgccg gttgaagccg cagccgtcat tgtggatttg gcgaaacgta
    12601 tgctggagca gaaagcatcc ggcatcaaca tgacccgata aggaggcatc atgccagctt
    12661 tagatttgat tcgaccgtcg gtaaccgcca tgcgggtgat tgcctccgtt aacgctgaat
    12721 ttgcgcgtga actaaaattg ccgccgcata ttcgtagcct cgggctgatt tctgctgatt
    12781 ccgatgacgt cacctacatt gccgctgatg aagcgaccaa gcaggcgatg gtggaagtgg
    12841 tatatggtcg ctcgctgtat gcgggcgctg cacacggccc gtcaccgact gccggtgaag
    12901 tgctgattat gcttggtggg ccaaacccgg cggaagtgcg tgccggtctg gatgcgatgg
    12961 ttgcacatat tgaaaatggt gcagcattcc agtgggccaa tgacgcagaa aacaccgcct
    13021 tcctggcgca tgtggtttcg cgtaccggtt cttatctctc atcaaccgcc gggatcacgc
    13081 ttggcgatcc gatggcgtat ctggtggcac cgccgctgga agcgacctat ggcattgatg
    13141 cagcgttgaa atctgccgac gtacagctgg tgacctatgt cccgccaccg tctgaaacca
    13201 actactcggc agcattttta accggtagcc aggccgcgtg taaagcagcc tgtaacgcct
    13261 ttaccgatgc agtgctggaa atcgcgcgta atccaatcca gcgtgcgtaa cggaggttgc
    13321 cgatgatcaa tgcactggga ttgctggaag tggacggcat ggtcgccgcg atagatgcag
    13381 cggatgccat gctcaaagca gctaacgttc gtctgctcag tcacgaagtg cttgaccctg
    13441 gtcgcttaac gctggtggtg gaaggcgatc tggcggcgtg tcgtgcggcg ctggacgcag
    13501 gttgtgctgc cgcgatgcgt accgggcgtg tcatcagccg caaggagatc ggtcggccag
    13561 acgatgacac ccagtggctg gttactggct ttaaccgcca gccgaagcaa cccgtaaggg
    13621 aacccgacgc gccagttatc gtcgcggaat ctgctgacga gttgttggcg ctgttaacat
    13681 cagtacgtca gggaatgacg gcaggagaag tggctgccca ctttggctgg ccgctggaaa
    13741 aagccagaaa tgcgctcgaa cagctctttt ctgccgggac gttacgtaaa cgcagtagtc
    13801 gttatcgtct caagccccat taacctgtcg gaggtgccgg gtgtcctaca acacccggca
    13861 ttaacatcat gaaaaagacc cgtacagcca atttgcacca tctttatcat gaacccttac
    13921 ccgaaaacct gaagctcacg ccgaaggtcg aagtggataa tgttcatcaa cgacagacaa
    13981 cggatgtcta tgaacatgct ttaacgatta ccgcctggca gcagatttac gatcagctgc
    14041 atccgggcaa gtttcatggt gaatttacgg aaattctact cgatgatatt caggtttttc
    14101 gtgaatacac cggtctggcg ctgcgtcagt cgtgcctggt ctggccgaac tcgttctggt
    14161 ttggcattcc ggcgacgcgc ggtgagcagg gatttatcgg ttcgcaatgt ctgggaagcg
    14221 cggaaatcgc cacccgccct ggtggcactg aatttgaact gagcacgccg gatgattaca
    14281 cgatcctggg cgtggtgctt tctgaagatg tcatcacccg gcaggctaac tttttgcata
    14341 acccggatcg ggtattacat atgctgcgta gccagtcggc gctggaagtg aaagagcagc
    14401 ataaagccgc gctgtggggc tttgtccaac aggcgctggc gacgttttgc gagaacccgg
    14461 aaaatctcca tcagcctgca gtgcgaaaag tgctggggga taatttgcta atggcgatgg
    14521 gggcgatgct ggaagacgcg caaccaatgg tgacggcgga aagcatcagt catcagagtt
    14581 accgtagatt actttcccgc gcccgtgaat atgtgctgga aaatatgtct gagccggtga
    14641 cggtgctgga cttgtgtaat caactgcatg tcagtcgccg cacgctacaa aacgcgtttc
    14701 acgctatttt aggcattggc ccaaacgcgt ggctgaaacg cattcgcctg aacgccgtac
    14761 gccgcgaact gataagcccg tggtcgcaaa gcacaacggt aaaagacgcc gccatgcagt
    14821 ggggattctg gcatctgggg caatttgcca cggattacca gcagctgttt gccgagaagc
    14881 cgtcgttgac gttgcatcag cggatgcggg aatgggggtg agggagttac acccaatccc
    14941 tgaccttaat aaactcactt aacgccgctt ctgggctgcc atcttttggc tgataatcat
    15001 actcccagcg taccagtggc ggcattgaca tcaggatcga ctcggtgcgc ccgccagttt
    15061 gcaggccaaa cagagtgccg cgatcccaga ccagattgaa ctcgacataa cgaccgcgac
    15121 ggtagagctg aaaattgcgc tcgcgctcgc cgtaggccat cgctttacgt cgctctacaa
    15181 ttggtaaata agcgtcggtg tagcctttgc ctaccgcctg cataaaggca aaacagtggt
    15241 cgaaatctgg cgtgttcaga tcatcaaaga acagcccgcc aataccgcgc tgttcgttgc
    15301 gatgtttgag gtagaagtaa tcgtcgcacc actttttgta acggggataa acgtcttcac
    15361 caaatggcag gcacaggtca cgggcggtgc ggtgccagtg aatggcgtct tcttcaaaac
    15421 catagaaagg ggttaaatcg aagccgccgc caaaccacca gacgggctct gcacccggtt
    15481 tttcggcaat aaaaaaacgc acattcgcgt ggctggtggg aacatacggg ttatgcggat
    15541 gcactaccag tgaaacgccc atcgcctcga aactgcgccc ggcaagttcc gggcgatgag
    15601 cggtggcgga agctggcatc gcctcgccgt ggacgtgaga aaagttgacg cctgcctgtt
    15661 cgaaaacacc accattacgc aacacccgac tacgcccgcc gccgccagct tcgcgctgcc
    15721 aactatcttc gacaaattct gcgccatcga cggcggtcag ctgctgacaa atcgtatcct
    15781 gaaggttgag cagaaactgt ttaacctggt gtgcgtcggg tttcataact tatcgctttt
    15841 tcgaatgtgc tttctggttg tcgaaccagt ggaaataact gatcacgcct tcagcaatcg
    15901 ctgtggcgat tttctgacga aacgccgccg tgcctaacag ccgctcttct tccgggttgg
    15961 tgataaacga ggtttccacc agcaccgaag gaaccgacgg cgatttcaac accacaaacg
    16021 cggcttgttc ggtgttgcgg ctgtgcagtt tatgcaccgg cttaatcttt ttcagaatat
    16081 gcgagccgag cgtcaggctg tttttaatag tatcggtttg caccagatca aacagcactt
    16141 gctgcaatag gtgatccttg tcagtcgcct ttttaccggc aacttcatcg gcgcggtttt
    16201 cgcgttcaga caggtatttc gccattgcgc tacttgcccc acggttagag agggcaaata
    16261 ccgaagcacc ggcagctttc gggttggtaa agccatcggc atgaattgac ataaacagat
    16321 ctgcgccatg tttatgggcg atttcaacgc gatcgtaaag tgggataaac gtatcgccag
    16381 aacgcgttaa acgcgcatca atcccatgat tacgcaaaat ggaacggacg tttttagcaa
    16441 tcgccagcac cacatgtttt tctttcgaac cgttgcgtcc gatcgctccg gtatcaattc
    16501 cgccgtggcc tggatcgaga acaacgacac gtttgccgcc agattttttg gctttcggct
    16561 tgctgtgtcc gttgctggtt tttaaaggtt cgtctttggc gatggcttgc gacattcctg
    16621 acaacgtcag ggcagccaaa ccggctttca gcacctggcg gcgcgaagtg agtgttttta
    16681 gtggtttaaa agtgctcata cggcctgagt tgtaataata aagttccaga tgttatatcg
    16741 tatcgcgtaa tccgttacga cggtttgatg ttgagaattg ttttactttt catttcaata
    16801 gttgacaact ccccattatg ccactttttc aaccgatttt cgtcagatat tggctaattc
    16861 gaccgttcac gccataatca gccttttaac ccgcaaaaag gtaacgaata ccatggagat
    16921 acgcgtattt cgccaggaag atttcgaaga ggtcatcacc ctttgggagc gttgcgactt
    16981 gctgcgtccg tggaacgatc cggaaatgga catcgagcgt aagatgaacc atgacgtcag
    17041 tttgtttttg gtcgctgagg taaacggtga agtggtcgga acggtgatgg gcggttatga
    17101 cgggcatcgc gggtctgctt attatcttgg cgtgcatcca gagtttcgtg ggcgtgggat
    17161 tgccaatgcg ttgcttaatc ggctggagaa aaagctgatt gctcgtggct gcccgaaaat
    17221 tcagatcaat gtgccggaag ataacgacat ggtgctcgga atgtatgaac gcctgggata
    17281 tgaacacgcc gacgtgctga gtctgggtaa gcgtttgatt gaagatgaag agtactgagt
    17341 tccatcctgt ccattatgat gcgcacggac gcctgcgttt acccttgctc ttctggcttg
    17401 tgctattgct tcaggcgcga acctgggtgc tgtttgtcat tgccggtgcg tcgcgtgagc
    17461 agggtactgc gctgctaaat ctgttttatc ccgatcacga taatttctgg ctggggctga
    17521 ttcctggcat tccggcggtg ctggcgtttt tgttgagcgg tcggcgggct acgtttcctc
    17581 gcacctggcg tgtactctat tttctgttgc tgttggcgca ggtggtttta ctttgctggc
    17641 aaccgtggct gtggctgaac ggtgaatccg ttagcggtat cggtctggcg ctggtagtgg
    17701 cggatattgt ggcgttaatc tggctgctga ccaatcgacg tttacgcgct tgttttaatg
    17761 aagtaaaaga ataacggcac tttttggtga atttgcactc caagcaacgt tattgaataa
    17821 ccaaaggaag tgacatgaaa tcgctgcgtt taatgttatg cgctatgcca ttgatgctga
    17881 ccggctgttc cacgatgtcg tcagttaact ggtctgccgc taacccgtgg aactggtttg
    17941 ggtcgtccac caaagtgagc gagcagggcg ttggtgaatt aacggcgtcc acaccactgc
    18001 aagaacaagc cattgccgat gcgcttgatg gcgattatcg cctgcgcagc ggaatgaaaa
    18061 ccgcgaacgg caacgtggtg cgcttttttg aagtgatgaa gggcgacaac gtggcgatgg
    18121 tgattaacgg cgatcaaggc acgatcagtc gcattgacgt gctggatagc gatattcctg
    18181 ctgacaccgg tgttaaaatc ggtacaccgt ttagcgacct ttacagcaaa gcatttggca
    18241 attgccaaaa agctgatggt gatgataatc gtgccgtcga atgtaaagcc gaaggcagtc
    18301 aacatattag ctaccagttc agcggggaat ggagtggtcc tgaagggtta atgccttcgg
    18361 acgataccct gaaaaactgg aaagtcagta aaattatctg gcggcgttaa tttgcgtctg
    18421 aacaaaccgc cgccgcgaaa aaacacgtaa aatagcgctc aacaatgcca cggattgcgt
    18481 ggcattatta ttttcaggag gaacaatgtc tcaggttcag agtggcattt tgccagaaca
    18541 ttgccgcgcg gcgatttgga tcgaagccaa cgtgaaaggg gaagttgacg ccctgcgtgc
    18601 ggccagtaaa acatttgccg acaaactggc aacttttgaa gcgaaattcc cggacgcgca
    18661 tcttggtgcg gtggttgcct ttggtaacaa cacctggcgc gctctgagcg gcggcgttgg
    18721 ggcagaagag ctgaaagatt ttccgggcta cggtaaaggc cttgcgccga ccacccagtt
    18781 cgatgtgttg atccacattc tttctctgcg tcacgacgta aacttctctg tcgcccaggc
    18841 ggcgatggaa gcctttggtg actgcattga agtgaaagaa gagatccacg gcttccgttg
    18901 ggttgaagag cgtgacctga gcggctttgt tgacggtacg gaaaacccgg cgggtgaaga
    18961 gacgcgtcgc gaagtggcgg ttatcaaaga cggcgtggat gcgggcggca gctatgtgtt
    19021 tgtccagcgt tgggaacaca acctgaagca gctcaaccgg atgagcgttc acgatcagga
    19081 gatgatgatc gggcgcacca aagaggccaa cgaagagatc gacggcgacg aacgtccgga
    19141 aacctctcac ctcacccgcg ttgatctgaa agaagatggc aaagggctga agattgttcg
    19201 ccagagcctg ccgtacggca ctgccagtgg cactcacggt ctgtacttct gcgcctactg
    19261 cgcgcgtctg cataacattg agcagcaact gctgagcatg tttggcgata ccgatggtaa
    19321 gcgtgatgcg atgttgcgtt tcaccaaacc ggtaaccggc ggctattatt tcgcaccgtc
    19381 gctggacaag ttgatggcgc tgtaagcgtt tctgattcat tgattaaagg ccaggcagta
    19441 acattactga ctggcctttt tattactttt gctttaacgc cgcatacacc tgatcaacta
    19501 cccaaccata cgttgcaatc ggcagctgac cgctttcgaa catattggga ttcttttgcg
    19561 gatcggcaac cggcgaatgt ggcttgttgc ttaacatcac aatcgccata tgattcaccg
    19621 gatcgataac ggtcaccgtt ccggtccagc cagtgtgacc gtaggtttgc gggcttgcca
    19681 gcgtgccaaa cgtcggcgtc atggtggcat taccattcac gcgccagccg aggccaaaag
    19741 tggcatcttc cttagagctg gtggtgaaca tcttcaccgt ttccgcactg aacagctgca
    19801 catcgccata gcccccgccg ttcagcatcg tttgcattaa caccgcaata tcgccggtat
    19861 tggaaaacaa acctgcgtgc ccggaaacgc cgcccatcga ataaaaggct ttttcatcgt
    19921 gcacctgacc ccagagagtg gaggtgcgga tattcgggaa atggatcacg ccatcgcggg
    19981 tattgccgtt caattccgtg gcggcaattt gttgcggttt aaagcctttc agcagcgggt
    20041 taaataccgt atgtgtcagg ccgagcgggc gataaatcga ctcttcaaca tagcggtcaa
    20101 gcggctgacc ggtgaccgac tcgacgataa atccaagcag catataatcg acatcactgt
    20161 aaatatgctt gctgccgggc tgatactcta gcggcgtgcg cttgatcatc tccagcgtct
    20221 ggcctttatc ctgggaatat aacgcgcccg cgaccgcttt attcgggtat tgcggatctg
    20281 ccgggaaacc gccgctgtga tgcagcaggt cagaaatccg cagggtgttt ttgcctttaa
    20341 tggtgtcatt tggactatcg gcaaaccccg gaatatattt cgcaatccga tcgtcaggat
    20401 gcagtttgcc ttcgctcatc agcttttgca gggcgaagtt cgtggcgtac attttggtgt
    20461 ttgaggccag gtcatacagc gtcccggtgg tggctttgac aggctgttcc atcaacacgc
    20521 tgccatcgta ctttttcgcc gctccccagg ctttgcgata cacaatctga ttatctttaa
    20581 tgatcagcag gtttacgccg ggataaccgg cgtccacttg ctggctaatc cagcgatcca
    20641 tctggttaag ccgttcgacg ttgaaccctg ctttctctgg cgaactttct gtcagaacag
    20701 ggtattttgc ggcgctgacg ctacaactaa cagccagcag agaaagatag agcattgtcc
    20761 gtttcatgat atcagtccag attgacgtta cggcagccaa agagcgtggt gaaaataaac
    20821 ccgcaaaccc atgccaccag aatcccgcca gcataaaccg ccattgccgg aaggatgcct
    20881 tgtgcggaag tcatcagcgg cagcgccacc agaccagacg gcccaaaggc gctgtttaag
    20941 cccatcggca gaccccacca ggctatcagg ccgataaaca aaccgcccgc tgcgccgcct
    21001 aaacaggcgg taataaacgg tttcatgcgg ggcagggtga cgccgtaaat caacggttca
    21061 ccaacgccca gcagaccggg aataatcgcc ccgcgtacct gactgcgtaa tgcactgtgc
    21121 ggttgcgccc gccagtagag tgccagcgcc gcgcccacct ggcccgcgcc tgccattgaa
    21181 aggatgggaa ataagctgtt gaatccctgg ctgtccatta acgcgaggta aacaggaata
    21241 aagccctgat gcacgccaaa taccacggcg atcaggaaca gcccggctaa aaccgcacaa
    21301 ccgaacggat tactgttcag gtgcataaac agccatgaca taccttcgaa taaccagcca
    21361 cccagcggca taatgatcag gtacgcaagc gtagcggtga tcagcagggt gattaacgag
    21421 gtcagcagca tgtcgagatc gtccggcata aagcggcgca ccatgccttc aatgcgggcg
    21481 caggcccacg cggcaatcag cacgccgata atattgccgc gcggatcgat gggcagacca
    21541 aagaaatcgt gaaaaccggc gtagtaaccg gtggttgcag cggggttata accgagcaaa
    21601 aagagtgcgg caataattgc gccattgacg cccgtgccgc caaatgcctg ggcggcgtta
    21661 tagcccacca gaatcaccag gaaagtgaac aaacctttgc tgaacacctt cataaaattc
    21721 agcgcgtcgg gtagcgtgcc ctgagcatct gccggaacgt gcatcaccgt ggcaattaac
    21781 gtcgctatcc ccagcagcag accggcggca ataaaaccgg ggatcagcgg cgtgaagatg
    21841 gtggcgaatt tggcgagaaa ttgttgcacg ccggaggttt gtttggcttt taactgacgt
    21901 ttgttctggg cggcgatttc tgcggcatcc tgtaccggtg cctcgcccag cagctcgctc
    21961 atcgcttttg cggcacgatg cgctttacca ggcccaaaaa cgacctgcac ctggtcgctg
    22021 gtcaaaatga cgcccttcac gccttccagc gttttgatat tgggatcaac cagtgaactg
    22081 tcatgtacac ccagacgcag gcgcgtcata cagttaccac aactggcgat atttcccggt
    22141 ccgccgacac gggtaagaat ggtgttcaga agttcactgc tgatctcttt ggccatgggt
    22201 tattccttgt ctaaaacctg acgaataaag ccgccgtgtt gatccaggcg ttttttagct
    22261 tctgcggcat cgagattttt cagcaccatc acaatggccg ttttacagtt gcgctcgcag
    22321 gcaattaacg ccgcttccgc ttgttcggca ttacatccgg tggcgttttt aacgatattg
    22381 acctggcgca catgcagttt ttcgttggtg gcgaccacat cgaccatcag gttgccgaac
    22441 actttgccag atttaatcat cagcccggtg gaaagcatat tgagcaccag tttctgcgct
    22501 gtgcctgctt tcattcgcga agaaccagtg accacttcgg cacctacaat cggtgtaatg
    22561 gcaaactcgg cggtggtgga aacggcactc cccggattac aggaaattcc cactgtgcgg
    22621 cagccgagct ggcgtgcgta ttccagtccg gcaatcacat acggcgtgcg accgctggca
    22681 gcaatgccaa ccaccacatc ctgtgccgtt aaattaatat ttttcagatc gttaacacca
    22741 ccttcccggc tatcttcagc gccttccacc gcgtgctgaa tggcatattc gcctccagca
    22801 atcaaaccaa ccaccagacc cggtttcacg ccgtaggtgg gcggacattc gctggcatcc
    22861 agaatcccca gacgaccgga tgttcccgca ccgagataaa tcagacgacc gccgccgctg
    22921 acctgggcgt ggataacatc gatcgccgcg gcgatatccg gcaggacgcg ctcaacggca
    22981 agcggtacgg ttttatcttc atcgttgata atccggcaca tttccagcgt cgatacgcgg
    23041 tcaatttcag ccgaggcggt gttcgagcct tcagtaatca tcttttctag ttgcatcggt
    23101 ggtgacctta ctatttacga tcaaataatt gcagatgtat ggaatcatat atcccgaatt
    23161 gacagactta aaggaatcat tgagtctaca ctcgcagagt gtgaagagcg tcataagaac
    23221 ttaagcggaa tttatgctgc cagcccagga gagtggaaga tgttgtacct gacgaaaata
    23281 agcaatgcgg ggagtgaatt caccgaaaac gagcaaaaaa tcgctgactt tctgcaggcc
    23341 aacgtgagtg agctgcaatc cgtttcttca cggcagatgg cgaaacagct cggcattagc
    23401 cagtcgagca tagtgaaatt tgcccaaaaa ctcggcgcgc aaggatttac tgaattgcgt
    23461 atggcgttaa taggcgaata cagtgccagc cgggagaaaa cgaatgccac ggcgctgcac
    23521 ctgcacagtt cgattaccag tgatgattcg ctggaagtga ttgcccgcaa attgaatcgc
    23581 gaaaaagagc tggcgctgga gcaaacctgt gcgttgttcg actacgcgcg gctgcaaaaa
    23641 atcattgagg tcatcagtaa agcaccgttt atccagataa cgggcctggg cggatcggcg
    23701 ctggtggggc gcgatctgtc gttcaaactg atgaaaattg gctatcgcgt ggcctgcgaa
    23761 gccgacacgc acgtacaagc caccgtttcc caggcgttga aaaaaggcga tgtacaaatc
    23821 gctatctctt acagcggcag taaaaaagag attgtcttat gcgcagaagc ggcacgaaag
    23881 cagggggcaa cggtgattgc cattacttct ctggctgatt ctccgctgcg gcggctggcg
    23941 catttcacgc tcgataccgt ttccggtgaa accgaatggc gtagctcgtc gatgtctacc
    24001 cgcacggcgc aaaattctgt taccgattta ctgtttgttg gtcttgtgca actcaatgat
    24061 gtggagtcat tgaaaatgat tcagcgcagc agtgagctaa cacaacgcct gaaatgatgc
    24121 ataaagcagc gactggattg agattttcct gaattagtga gctgatccgc agcaatattt
    24181 tgtttatcct gtattttcag agggaatgga gtgtaacgct ctgtattaac aaggagagca
    24241 ttaaaatggg taaactcacg ggcaagacag cactgattac gggcgcattg cagggaattg
    24301 gcgaaggaat tgccagaact tttgcgcgtc atggcgcaaa cctaatcttg ctggatatct
    24361 cccctgagat cgaaaagctg gcggacgaac tgtgtggtcg tggtcatcgc tgtacggcgg
    24421 ttgtcgccga tgtgcgtgac ccggcgtcgg tagccgcagc tatcaaacgc gcgaaggaaa
    24481 aagaagggcg cattgatatc ctggtgaata acgcaggcgt ttgtcgtctg ggcagtttcc
    24541 tcgatatgag cgatgacgat cgcgatttcc atattgacat caatattaaa ggcgtatgga
    24601 acgtcacgaa ggcggtgctg ccggagatga ttgcccgcaa agatggtcgc attgtgatga
    24661 tgtcttcggt cactggcgat atggtggccg atcctggcga aacggcgtac gccttaacga
    24721 aagcggcgat tgttggcctg acaaaatcgc tggcggtgga gtacgcgcag tctggtattc
    24781 gcgttaacgc catttgcccg ggatacgtgc gcacaccaat ggcggaaagc attgcccgcc
    24841 agtcgaaccc ggaagatcca gagtcggtgc tgactgaaat ggcgaaagca atcccgatgc
    24901 gtcgcctcgc cgatccgctg gaagtcggcg aactggcggc cttcctcgca tcggatgaat
    24961 ccagctattt aaccggtaca cagaatgtga ttgatggcgg cagcacactg ccggagacgg
    25021 ttagcgtcgg tatctgattc acctctgttt cctccctgca tttgtgggga ggatttcgtt
    25081 ttaaacccgg ttcttattcc cttttcaact tccaaatcac caaacggtat ataaaaccgt
    25141 tactcctttc acgtccgtta taaatatgat ggctattaga aagtcattaa atttataagg
    25201 gtgcgcaatg gccgttaact tactgaaaaa gaactcactc gcgctggtcg cttctctgct
    25261 gctggcgggc catgtacagg caacggaact gctgaacagt tcttatgacg tctcccgcga
    25321 gctgtttgcc gccctgaatc cgccgtttga gcaacaatgg gcaaaagata acggcggcga
    25381 caaactgacg ataaaacaat ctcatgccgg gtcatcaaaa caggcgctgg cgattttaca
    25441 gggcttaaaa gccgacgttg tcacttataa ccaggtgacc gacgtacaaa tcctgcacga
    25501 taaaggcaag ctgatcccgg ccgactggca gtcgcgcctg ccgaataata gctcgccgtt
    25561 ctactccacc atgggcttcc tggtgcgtaa gggtaacccg aagaatatcc acgattggaa
    25621 cgacctggtg cgctccgacg tgaaactgat tttcccgaac ccgaaaacgt cgggtaacgc
    25681 gcgttatacc tatctggcgg catggggcgc agcggataaa gctgacggtg gcgataaagc
    25741 caaaaccgaa cagtttatga cccagttcct gaaaaacgtt gaagtgttcg ataccggcgg
    25801 tcgtggcgca accaccactt ttgccgagcg tggcctgggc gatgtgctga ttagcttcga
    25861 atcggaagtg aacaacatcc gtaaacagta tgaagcgcag ggctttgaag tggtgattcc
    25921 gaaaaccaac attctggcgg aattcccggt ggcgtgggtt gataaaaacg tgcaggccaa
    25981 cggtacggaa aaagccgcca aagcctatct gaactggctc tatagcccgc aggcgcaaac
    26041 catcatcacc gactattact accgcgtgaa taacccggag gtgatggaca aactgaaaga
    26101 caaattcccg cagaccgagc tgttccgcgt ggaagacaaa tttggctcct ggccggaagt
    26161 gatgaaaacc cacttcacca gcggcggcga gttagacaag ctgttagcgg cggggcgtaa
    26221 ctgatgtttg ctgtctcctc cagacgcgtg ctgccgggct ttaccttaag cctcggcacc
    26281 agtctgctgt ttgtgtgcct gattttgctg ctgccgctct ccgcgctggt gatgcaactg
    26341 gcccagatga gctgggcgca gtactgggag gtgatcacca acccgcaggt ggtcgcggcc
    26401 tacaaagtaa cgctgctgtc ggcgtttgtg gcatcgattt ttaacggcgt tttcggtctg
    26461 ctgatggcgt ggatcctaac ccgctatcgc ttcccaggcc gcacgctgct tgatgcgctg
    26521 atggatttac cctttgcgct gccaacggct gtcgccggtt taacgctggc ctcgctcttt
    26581 tccgtaaacg gtttttacgg tgaatggctg gcgaagtttg atatcaaagt cacctataca
    26641 tggctgggga ttgcggtggc gatggccttt accagcattc cgtttgtggt gcgtaccgtg
    26701 cagccggtgc tggaagagtt aggcccggaa tatgaagaag cggcggaaac gcttggtgca
    26761 acgcgctggc agagtttctg caaagtggtg ctgccggagc tttctccggc gctggtggcg
    26821 ggcgtggcgc tgtcgtttac ccgtagtctt ggtgaatttg gcgcggtgat ttttatcgcc
    26881 ggaaatatcg cgtggaagac ggaagtgacg tcgctgatga tttttgtgcg cttgcaggag
    26941 tttgattacc cggcggcgag cgcgattgct tcggtgatcc tcgcggcatc cctgatactg
    27001 ctgttctcaa ttaacactct gcaaagtcgc tttggtcggc gtgtggtagg tcattaatgg
    27061 cggaagtgac acaattgaag cgttatgacg cgcgcccgat taactggggc aaatggtttc
    27121 tgattggcat cgggatgctg gtatcggcgt tcatcctgct ggtgccgatg atttatatct
    27181 tcgtacaggc gttcagcaaa gggctgatgc ctgttttaca gaatctggcc gatccggata
    27241 tgctgcacgc catctggctg acggtgatga tcgcgctgat tgccgtaccg gtaaacctgg
    27301 tgttcggtat tctactggcg tggctggtga cgcgctttaa cttccccgga cgccagttgc
    27361 tgctgacgct gctggacatt ccgtttgccg tgtcgccggt ggttgccggt ctggtgtatt
    27421 tgctgttcta cggctctaac ggcccgctcg gcggttggct cgacgagcat aacctgcaaa
    27481 ttatgttctc ctggccggga atggtgctgg tcaccatctt cgtgacgtgt ccgtttgtgg
    27541 tgcgcgaact ggtgccggtg atgttaagcc agggcagcca ggaagacgaa gcggcgattt
    27601 tgcttggcgc gtccggctgg cagatgttcc gtcgcgtcac attaccgaac atccgctggg
    27661 cgctgcttta tggcgtggtg ttgaccaacg cccgcgcaat tggcgagttt ggcgcggtgt
    27721 cggtggtttc cggctcgatt cgcggcgaaa ccctgtcgct gccgttacag attgaattgc
    27781 tggagcagga ctacaacacc gtcggctcct ttaccgctgc ggcgctgtta acgctgatgg
    27841 cgattatcac cctgttttta aaaagtatgt tgcagtggcg cctggagaat caggaaaaac
    27901 gcgcacagca ggaggaacat catgagcatt gagattgcca atattaagaa gtcgtttggt
    27961 cgcacccagg tgctgaacga tatctcactg gatattcctt caggtcagat ggtctcgttg
    28021 ctggggccgt ccggttccgg gaaaaccacg ctgctgcgca ttatcgccgg gctggagcat
    28081 caaaccagcg ggcatattcg cttccacggc accgacgtca gccgcctgca cgcgcgcgat
    28141 cgcaaggtcg gtttcgtgtt ccagcattac gcgctgttcc gccatatgac ggtattcgac
    28201 aatatcgctt ttggcctgac ggtgctgccg cgtcgcgatc gcccgaatgc cgcagccatc
    28261 aaagcgaaag tgacaaaatt gctggagatg gtgcagcttg ctcatctggc agatcgttat
    28321 ccggcacagc tttccggcgg ccagaaacag cgcgtggcgc tggcgcgcgc gctggctgta
    28381 gaaccgcaaa ttctgctgct tgatgaaccg tttggcgcgc tggatgcgca ggtgcgtaaa
    28441 gagctgcgtc gctggctgcg tcaactgcat gaagagctga aattcaccag cgtgttcgtg
    28501 acccacgacc aggaagaggc gaccgaagta gccgatcgtg tagtggtgat gagtcagggg
    28561 aatattgaac aggctgatgc gccgaatcag gtatggcgcg aaccagcgac ccgttttgta
    28621 ctcgaattta tgggcgaagt gaaccgcctg cagggaacca ttcgcggcgg gcagttccat
    28681 gttggcgcgc atcgctggcc gctgggctac acacctgcgt atcagggtcc ggtggatctc
    28741 ttcctgcgcc cgtgggaagt ggatatcagc cgccgtacca gtctcgattc gccgctgccg
    28801 gtacaggtac tggaagccag cccgaaaggt cactacaccc aattagtggt gcagccgctg
    28861 gggtggtaca acgaaccgct gacggtcgtg atgcatggcg acgatgcccc gcagcgtggc
    28921 gagcgtttat tcgttggtct gcaacatgcg cggctgtata acggcgacga gcgtatcgaa
    28981 acccgagatg aggaacttgc tctcgcacaa agcgcctgat aggttgagtg aatgttaaac
    29041 gcccggaggc gcttcccgcg atccgggctt tttaatggca aggtttgtaa cctgtagacc
    29101 tgataagacg cgcaagcgtc gcatcaggca acaccacgta tggatagaga tcgtgagtac
    29161 attagaacaa acaataggca atacgcctct ggtgaagttg cagcgaatgg ggccggataa
    29221 cggcagtgaa gtgtggttaa aactggaagg caataacccg gcaggttcgg tgaaagatcg
    29281 tgcggcactt tcgatgatcg tcgaggcgga aaagcgcggg gaaattaaac cgggtgatgt
    29341 cttaatcgaa gccaccagtg gtaacaccgg cattgcgctg gcaatgattg ccgcgctgaa
    29401 aggctatcgc atgaaattgc tgatgcccga caacatgagc caggaacgcc gtgcggcgat
    29461 gcgtgcttat ggtgcggaac tgattcttgt caccaaagag cagggcatgg aaggtgcgcg
    29521 cgatctggcg ctggagatgg cgaatcgtgg cgaaggaaag ctgctcgatc agtttaataa
    29581 tcccgataac ccttacgccc attacaccac taccgggccg gaaatctggc agcaaaccgg
    29641 cgggcgcatt acccattttg tctccagcat gggaacaacc ggcactatca ccggcgtttc
    29701 acgctttatg cgcgaacaat ccaaaccagt aacgattgtt ggcctgcagc cggaagaagg
    29761 cagcagtatt cccggcattc gccgctggcc tgcggaatat ctgccgggga ttttcaacgc
    29821 ttctctggtg gatgaggtgc tggatattca tcagcgcgat gcagaaaaca ccatgcgcga
    29881 actggcagtg cgggaaggaa tattctgtgg cgtcagctcc ggcggcgcgg ttgccggagc
    29941 actgcgggtg gcaaaagcta accctggtgc ggtggtggta gcgatcatct gcgatcgtgg
    30001 cgatcgctac ctttccaccg gggtgtttgg ggaagagcat tttagccagg gggcggggat
    30061 ttaagattat tgcgcaggca ccgttttatc cgtctgcgca attagcgcat gtaaaaaagg
    30121 ctgtgcgttc tcatcgctct taccggggat cttcacaata tacggcttcc cggtaatcga
    30181 tgacgacgaa gcaaccttat caataaactg ctcggcagtg tcaatgcggt tacgggtatt
    30241 gcccagcttc agacgcagat gagaaaccgc ttcatcgcag gtatgttcat caccgttgcg
    30301 cacaaatatc aaatcctttt tctgtgctaa tccctccagc atggcgttga tgcgggcttc
    30361 ttcatgggca gttaacttcg cgtaagcggg gagtgtcatt agtagtgtaa tgaccagaca
    30421 aatgattttc ttcattgaat taccttatcc ttgttttgtg atagccgatt tgactgccag
    30481 aatcaggtaa agtttcggtt ggaatacgat gaggatcatc ggcaaggaga aaaaattaaa
    30541 tgagtagttt gttgttgttt aacgataaga gcagggcgct gcaggcggat atcgtcgccg
    30601 tgcagtcgca ggtggtttac ggcagcgtgg gcaacagcat tgccgtgcct gctatcaaac
    30661 agaacggtct gaatgttttt gccgtgccga cggtattgct gagcaatacg ccgcattatg
    30721 acactttcta cggtggtgcg attccggatg aatggtttag cggttatttg cgtgcgcttc
    30781 aggagcgtga tgcgctgcgc cagctgcgcg ctgtaaccac gggctatatg ggaacggcat
    30841 cgcaaatcaa aatccttgcc gagtggctga ctgcgctacg caaagaccat cctgacctat
    30901 tgatcatggt cgatccggtg attggcgata ttgatagcgg aatttatgtc aaacctgacc
    30961 ttcccgaggc gtatcgacaa tatttactgc cgctggcgca gggaattacc cccaatatct
    31021 ttgagctgga aatcctgacc ggtaaaaatt gccgcgatct cgacagtgcc attgctgccg
    31081 caaaaagtct gctttcagac acattaaaat gggtggtgat taccagcgcc tccggtaatg
    31141 aagaaaatca ggagatgcag gtggtggtgg tcagtgccga cagcgtgaat gtcatttccc
    31201 attcacgggt aaaaaccgac ctgaaaggga ctggcgacct gttttgtgct cagcttatca
    31261 gtggtttgct gaaagggaag gcgttaaacg atgcggtgca ccgtgcgggg ttgcgcgtac
    31321 tggaagtgat gcgctacacc cagcagcatg agagcgatga attgattttg ccgccgctgg
    31381 cggaagcata aaaaatggcg ccgatgggcg ccatttttca ctgcggcaag aattacttct
    31441 tgatgcggat aaccggggtt tcacccacgg ttacgctacc ggacagtttg atcagttctt
    31501 tgatttcgtc catgttggag ataacaaccg gagtcagggt agacttggct ttctcttcca
    31561 gtagcggcag atcaaattca atgacagtat cgccaacttt cacgcgctga ccttcttcag
    31621 caatacgctt gaagccttcg cctttcagtt caacggtgtc gataccgaag tggacgaaca
    31681 gttcaacgcc gctatcagat tcgatagaga acgcgtgatt ggtttcaaag attttaccaa
    31741 tggtaccgtc tacaggcgcg accattttgt tacccgttgg tttgatagca ataccatcac
    31801 caacgatttt ttccgcaaaa acgacatccg gcacgtcttc gatattgacg atctcgccag
    31861 agagcggagc aatgatctca atagttccgg tatccttctt gtcgtcggaa accagagatt
    31921 tcagtttatc gaacaaaccc atgatcttct cctaagcagt aaattgggcc gcatctcgtg
    31981 gattagcaga ttgttttttc ttcaatgaac ttgttaacca gcgtcattaa ctcgtccgtt
    32041 gtcggttgag caagagcctg ctctgctaac accttcgcat cttcgaagtt cgtgttacgg
    32101 ataatcttct taatgcgcgg gatagaaatg gcgctcatag agaattcgtc cagacccatc
    32161 cccagcaaca gaagtgtagc acgttcatcg ccagcaagct caccacacat gccagtccat
    32221 ttgccttcag catgagaagc atcaataact tgcttgatca agttcagcac ggacggtgac
    32281 attggttggt aaaggtgtga aatcatatca ttaccacggt caactgccag agtgtactgc
    32341 gttaaatcat tggtgccgat actaaagaaa tcaacttctt tggctaaatg acgtgcaatt
    32401 gttgcggcag ccggtgtttc caccattacg ccgatttcaa ttgactcgtc aaacgcttta
    32461 ccttcgtcgc gcagttcctg tttgtagatt tcgatctctt tgcgcagtgc acgcacttct
    32521 tcaacagaga tgatcatcgg gaacataatg cgcaatttac cgaaagccga ggcacgcagg
    32581 atagcgcgga gctgatcgcg caggatctct ttacgatcca tcgcgatacg gatagcgcgc
    32641 cagccgagga acgggttctc ttctttcggg aagttcatgt atggcagctc tttgtcgccg
    32701 ccgatgtcca tggtacgaac gataaccgct tgcgagccac acgcttcagc cactgctttg
    32761 taagcagcaa actgttcttc ttcagtgggc agcgcgtcgc ggtccatgaa caggaactca
    32821 gtacgataca gaccaacgcc ttcagcgccg ttacgctctg caccttcaac gtcacgaacc
    32881 gtaccaatgt tagcgcatac ttctacctgg tgaccgtcca gcgtaatagc tggcagatct
    32941 ttcagtttag caagctctgc tttttcagaa gccacttgct cctgaacagc gcgcatttta
    33001 tcaataactt cgttggttgg attgacgtaa acctgattat ttacggcatc cagaatcaga
    33061 tagtcgtcat ttttcacctg agaggtgacg ctaccggtac ccacgatagc aggcagttcc
    33121 agagaacgcg ccatgataga ggtgtgggaa gtacggccac ccgcgtcggt gatgaaaccc
    33181 agcaccttct tcaggttcag ctgtgcggtt tcggacggcg tcaggtcagc ggcaaccaga
    33241 atgacttcat cctgaatggc gctcaggtcg ataatcttca ggcccaggat gttgcgcagc
    33301 aggcgcttac cgatatcacg tacgtcagcc gcacgttctt tcaggtattc atcatccagc
    33361 tcttccaggg cagaagcctg accttcgata acttcatgag cagctgcgtc agctgtcatg
    33421 tgcttatctt taatcagggc tatgatttcc tgctccagct cctcatcttc gagcagcata
    33481 atatgccctt caaagatggc ttctttttct tcaccgaacg tttcaccagc tttcgttttg
    33541 atcgtttcca gctgggctga tgccttggca cgaccgctca gaaaacgttc aacttcctga
    33601 tcaacctggt cggcagaaat ttttttccgg tcaatgacaa tttcgtcttc tttcagaagc
    33661 agagctttac cgaaagcgat acccggggat gctaaaatgc ctgaaatcat aaccctacct
    33721 tacttgtgac tgatttttaa aagaacccgg gaaattactc gagttccgcc atcagtttaa
    33781 ccagatgttc aaccgctttc tgctcgtctt cgccttctgc ggagatagtc acaacggtac
    33841 cttgagtcag gcccagagtc tgcagtttaa acaggctttt cgcgctggcg cttttgccgt
    33901 tggaagtcac agtaatttca gaagtgaagc ctttagcttc ttttacaaac tgggcagcag
    33961 ggcgggtgtg cagaccgttc ggagcggtaa tggtaacttc ttgctggaac attgtatttc
    34021 cccaacttat aggtttagtg ttgtggaact aaagtctagc ctggcggttc gactttagcc
    34081 tgtattgtta gcgccagttt ttaacagacg cgacgcacga agagcgcaat acagtccatt
    34141 gctggcgggt gttgaccgct gacgtttcgt acgtcattaa acattatgcc gctaaaggaa
    34201 gaattgaacc aaatcataaa atcgattcag ctttggcttt tcctgtaacg attaatttcg
    34261 cgcatcaaaa taattagtct ggttaaatac cagttccgac aggctgaatc aatgccagga
    34321 ggggcgaaag tttgaagcag gccacaaaaa agcacctaaa aaggtgcttt tttacgcatt
    34381 tttaacaagc tggcattact gttgcagttc tttctcagtg aagagatcgg caaacaatgc
    34441 ggtgcttaaa taacgctcac ccgatgacgg tagaataacc acaatattct tgttggtaaa
    34501 gctttcatct tcttgtagct tcaacgccgc agcaacagct gctccagaag agatacctgc
    34561 aagaatacct tcttcttcca tcagacgacg tgcggtagaa atcgcttctt cattggtgat
    34621 gccaatgact ttatcgacca gcttgagatc gaggttagct gggataaaac cagcgccaat
    34681 accctgaatt ttatgcgggc caggtttaat ctcttcacca gccagtgcct gggcgataac
    34741 tggagaatcg gttggctcaa cggcgacaga gataagatcg gtcttgcctt tggtgccttt
    34801 aatgtagcgg ctgacgccag tcagcgtacc gccagtccca acgccagcaa taaatacatc
    34861 aacctgaccg tcggtatctt cccagatctc cggaccggtg gttttttcgt gaatttcagg
    34921 gtttgccgga ttgctgaatt gttgcagcag caggtatttc tctggattgc tggcgacaat
    34981 ttcttctgct ttttggattg cgcctttcat gcctttagca ccttccgtca gcaccaggtt
    35041 tgcacctaac gctttcagca gcttgcggcg ttcaatactc atggtttctg gcatggtcag
    35101 ggtgagtttg taaccgcgag cggcagctac ataggccagt gcaatcccgg tattaccgct
    35161 ggtcggttca accagttcaa cgcctggttt cagcacgccg cgcttttcgg catcccaaat
    35221 catgttggca ccgatacggc acttaacgct gaagctgggg ttacgagatt ccaccttcgc
    35281 cagaatgcgt ccgttaccga tgcgattcag gcgaaccagc ggcgtgtgac cgatagtcag
    35341 cgagttatct tcaaaaatct tactcatggc ctgtccttaa ctgtatgaaa ttgggataca
    35401 acaggtagca tacccgctca gagaatatgc ggaagtaagg atttagcata tctatataca
    35461 gaagggaaat aatgacatgc aagatggaat aaggggcggc ataagccacc cctgtttcac
    35521 acaaacggtt tacaaaataa ccggtagatt gttaccgcca catcgcgtgt ttatcgcgat
    35581 agcaatcgac ccacatcgcc gtcgcgccac aaacggcaac gggcatgatg aacagattaa
    35641 gcagcgggat catcgtaaac aggctggtta aagcaccaaa ctgcatattg gtgattttgc
    35701 gtgtgcgcag ggcggtgcgc atctctttaa acggcacttt gtggttatcg aaggggtaat
    35761 cgcaatactg gatggctaac atccaggcgc taaacaggaa ccacagtacc ggcgcgacgg
    35821 tttgcccaat accagggatt aagtaaagaa ttagcaatac aattgcgcgc ggcagatacc
    35881 aggcaaattt ttgccattcg cgtttcatga ttcgcggcac atctttcatg ataccgaaaa
    35941 tcccggtatc tggcggtgta gcgccagtca atcgtgcttc cagttgttca gccaataaac
    36001 cgttaaacgg agcggcaatc cagttagcaa tcgtggagaa gaaatagcca aacactaaca
    36061 gcacagagat gacagccaga ggccacaaca gataactcag ccattgtagc cagtccggaa
    36121 cgtaactcat gagagtcggg atccagacat cgagctgtgt aaagagccac cagaatgcgc
    36181 cccccatcaa caaaatattg accagcagcg gtaaaataac gaaacgccga atcccaggtt
    36241 gcgagacgag cttccagcct tgcgcaaaat agtaaaaacc gctgcgtggg gcagatgtga
    36301 atgatgaaac cataatcagg atgagctcct tttgaccaat cccaggaaaa ttctgcgtat
    36361 tttaccgggt aattgcgcaa tggacagtta ggatatgttc gaaaaaacag caaaaagcac
    36421 gatttcatct atctttgtgc tgtgaaagtt aatagtgcac ttgcacttga ggtaatcggc
    36481 aaatactctt agtgagtaaa tgtttgccgt ggtggcaagg tgttagaaca acagagaata
    36541 taatgatgca ggatttgcgt ctgatattaa tcattgttgg cgcgatcgcc ataatcgctt
    36601 tactggtaca tggtttctgg accagccgta aagaacgatc ttctatgttc cgcgatcggc
    36661 cattaaaacg aatgaagtca aaacgtgacg acgattctta tgacgaggat gtcgaagatg
    36721 atgagggcgt tggtgaggtt cgtgttcacc gcgtgaatca tgccccggct aacgctcagg
    36781 agcatgaggc tgctcgtccg tcgccgcaac accagtacca accgccttat gcgtctgcgc
    36841 agccgcgtca accggtccag cagccgcctg aagcgcaggt accgccgcaa catgctccgc
    36901 gtccagcgca gccggtgcag cagcctgcct atcagccgca gcctgaacag ccgttgcagc
    36961 agccagtttc gccacaggtc gcgccagcgc cgcagcctgt gcattcagca ccgcaaccgg
    37021 cacaacaggc tttccagcct gcagaacccg tagcggcacc acagcctgag cctgtagcgg
    37081 aaccggctcc agttatggat aaaccgaagc gcaaagaagc ggtgattatc atgaacgtcg
    37141 cggcgcatca cggtagcgag ctaaacggtg aactgcttct taacagcatt caacaagcgg
    37201 gcttcatttt tggcgatatg aatatttacc atcgtcatct tagcccggat ggcagcggcc
    37261 cggcgttatt cagcctggcg aatatggtga aaccgggaac ctttgatcct gaaatgaagg
    37321 atttcactac tccgggtgtc accatcttta tgcaggtacc gtcttacggt gacgagctgc
    37381 agaacttcaa gctgatgctg caatctgcgc agcatattgc cgatgaagtg ggcggtgtcg
    37441 tgcttgacga tcagcgccgt atgatgactc cgcagaaatt gcgcgagtac caggacatca
    37501 tccgcgaagt caaagacgcc aacgcctgat acacttaagg caaattaact cctcttcgaa
    37561 cccccgcttg tcgggggttt ttagcattga tggtgcgata tggaatcaat cgaacaacaa
    37621 ctgacagaac tgcgaacgac gcttcgccat catgaatatc tttatcatgt gatggatgcg
    37681 ccggaaattc ccgacgctga atacgacagg ctgatgcgcg aactgcgcga gctggaaacc
    37741 aaacatccag aactgattac gcctgattcg cctacccaac gtgtaggtgc tgcgccgctg
    37801 gcggctttca gccagatacg ccatgaagta ccaatgctgt cactggataa cgtttttgat
    37861 gaagaaagct ttcttgcttt caacaaacgt gtgcaggacc gtctgaaaaa caacgagaaa
    37921 gtcacctggt gctgtgagct gaagctggat ggccttgccg tcagtattct gtatgaaaat
    37981 ggcgttttag tcagtgccgc gacccgtggc gatggcacca ccggggaaga tatcacgtct
    38041 aatgtgcgta ctattcgcgc tattccgctg aagctgcacg gagagaatat cccggcgcgt
    38101 ctggaagtgc gtggtgaagt gttcctgccg caggcggggt tcgaaaagat taacgaagat
    38161 gcgcgacgca cgggcgggaa agtttttgct aacccacgta atgcggcagc tggttcactt
    38221 cgtcagcttg atccgcgtat tacagcgaag cgaccgctca cttttttctg ctatggcgtt
    38281 ggtgttctgg aaggtggcga gctgccggat actcatcttg gccgtttact gcaatttaaa
    38341 aagtgggggt tgccggtcag cgatcgggta acgctttgtg aatcggcgga agaagtgctg
    38401 gcgttctatc acaaagtgga agaagaccgc ccgacgctgg gctttgatat cgacggcgtg
    38461 gtgattaagg tcaactcact ggaacagcag gagcagcttg gctttgtcgc gcgtgccccg
    38521 cgctgggcgg tagcgtttaa attcccggcg caggagcaga tgacctttgt gcgtgacgtc
    38581 gagtttcagg ttgggcgtac tggcgcgatt acgcctgttg cgcgtctgga acctgtccat
    38641 gttgctggcg tgctggtgag taacgcaacc ttacacaatg cggatgaaat cgaacgtctt
    38701 ggtttacgca ttggcgataa agtggtgatt cgccgcgctg gcgacgtgat cccgcaggtg
    38761 gttaacgtcg tgctttctga acgcccggaa gatacccgtg aggttgtatt cccaacgcat
    38821 tgtccggtat gtggttctga cgttgagcgt gtggaaggtg aagcggttgc ccgctgtacc
    38881 ggtggcctga tttgcggtgc gcagcgtaaa gagtcgctga aacactttgt ttcccgccgt
    38941 gcgatggatg ttgacggaat gggcgacaaa atcatcgatc agctggttga aaaagaatat
    39001 gtccatactc cggcagatct gttcaaactc accgcaggca aactgaccgg actggagcgt
    39061 atggggccaa aatcggcaca aaacgtggtt aacgcgctag aaaaagcgaa agaaaccacc
    39121 tttgctcgct tcctctatgc acttggcatc cgtgaagtcg gcgaggccac cgcagcaggt
    39181 ctggcggcat atttcggcac gctggaagcg ctggaagccg cttcgattga agagctgcaa
    39241 aaggtgcctg atgttggcat tgtcgttgca tcccacgttc acaacttctt tgccgaagaa
    39301 agcaaccgca atgttatcag cgagctgttg gcggaaggtg tccactggcc tgcgccgatc
    39361 gttatcaacg cggaagagat tgacagcccg tttgctggta aaaccgtggt gcttacgggc
    39421 agcttaagtc agatgtcacg tgatgacgct aaagctcgac tggtcgaact gggcgcgaaa
    39481 gtcgcgggca gcgtgtcgaa gaaaaccgat ctggtgatag cgggtgaagc tgcaggatct
    39541 aaactggcga aggcgcagga actgggcatt gaagtcatcg acgaagcgga aatgctgcgt
    39601 ttgctgggta gctgagatgg aaaaagagca gctgattgaa atagccaata cgataatgcc
    39661 gtttggcaaa tacaaagggc gtcgcttaat cgacctgccg gaggagtatc tgctgtggtt
    39721 tgcccgcaaa gatgagttcc cggcagggaa gctcggtgag ctaatgcaaa tcacgctgct
    39781 gattaaaacc gaggggctga cgcaactggt ccagcccctg aagcgtccgc tttaagcttt
    39841 atcggtgctg ctttcctgct gcgcctgtaa ctgttcggtc tggcgtttgt atcgacgcgc
    39901 cagcaccgca cagaccatca attggatctg atggaaaatc atcaggggca gcaccatcat
    39961 accgatcacc gatgtgggga acagaatgtt tgccatcggg atgccatttg ccagactctt
    40021 tttcgaacca caaaagacga tagtaatttc atctgcctta ttgaagccca gtcggcgtgc
    40081 cataaagacg ttaactacaa tcacgatagc cagaagaacg cagctgacca ccacgataaa
    40141 cagcaatgat ccccagccaa ctttatgcca gataccatta acgacggctt cgctgaacgc
    40201 tgtataaacc accaacagaa tggacgtctg gtcagttttc gcaatccatt ttttattgcg
    40261 cgacacccag tcaccaatcc acggccggga aagatgcccc aacacaaacg gcagcagcag
    40321 ttgcagcata attttaccga cctgctcaag gctgccccct gcaccgtgaa cattcatcac
    40381 cagaccaacc agcaatggtg aaaggaaaat ccccagcagg ctggatgctg acgcagaaca
    40441 aaccgccgcc gcgacgttac cgcccgccat tgacgtgaag gcgattgcag actgcacggt
    40501 agccgggaga atgcacaagt agagaaaacc ggagtagagc atcgggtcga catttaccgg
    40561 tttccaccag gcaaacagta cacccagaat cggaaacagc acgaaggtgc tgcacattac
    40621 ccacaaatgc agtcgccagt gaccaccgcc agcaataatc gcctcacgcg acaacttcgc
    40681 gccgtgcata aagaacagca gggcaatcgc tgcggtggtc agattttcaa agaaggggac
    40741 gaaatcgcct ctggccggaa agaaagaggc cagcaacacc accgtgatca gggttaaggt
    40801 gaaaggatcg aggatacgaa aaagtttcat aaagactcca gatgatcgat ggttctattg
    40861 tgcttcttcc agtttgagaa ataaaattga tttattgcat ccattcatga agaaaacaga
    40921 tgaattattc tttaaaacaa ttaaaggttt tcgtcacagt agcgcaggag aaaagtttta
    40981 gtcgtgcagg agagcgtatc ggcctgagcc agtcggcagt gagtcacagt gtgaaggaac
    41041 tggaaaatca tactggtgtt cgcctgctgg acagaaccac gcgtgaagtg gtgcttacag
    41101 atgcagggca gcagctggct ttgcgtcttg agcgactgct ggatgaactg aacagcacgt
    41161 tgcgcgatac cgggcgtatg gggcaacaac tgagcggaaa agttcgggtc gctgccagcc
    41221 agaccatttc cgcgcatctt attccgcaat gcattgctga aagccatcgc cgctatccag
    41281 atattcagtt tgtcctgcac gatcgtccgc agcagtgggt gatggaaagt attcgccagg
    41341 gagatgtcga ctttggcatc gtcatcgatc caggcccagt gggcgatctg caatgtgaag
    41401 cgattctttc cgagcctttc tttctgcttt gccatcgcga tagcgctttg gccgtcgaag
    41461 attatgtacc ctggcaggca ttacagggag cgaaattggt gttgcaggat tacgcgtcag
    41521 gcagccgacc gctgattgac gcagcgctgg cgcgcaatgg tattcaggcg aatattgtgc
    41581 aggagattgg acatccggcg acgctgttcc cgatggtagc cgcaggcatc ggcatcagta
    41641 ttcttcccgc actggcgcta ccattgcctg aaggtagccc actggtggta aagcgcatta
    41701 cgccggttgt tgaacgccag ttgatgctgg tgcgcaggaa gaaccgttca ctttccaccg
    41761 ccgctgaagc gttatgggac gtggtacgag accagggcaa tgcgctaatg gctggtcggg
    41821 aaggggatcc gttgtatcag atatagagtg aattcatact gccgggagcc ccggcagtaa
    41881 tcaatcagaa gttaaatttg atggagtaga ttatcgcatc ctggtaaaaa tcttcgccca
    41941 gtttgtcatc cgcatatcga tactgaatcc ccgtagtaat gtgtgatgtt gcattccacc
    42001 acagtgcaac tgcgccattc agcccttctt taccgccatt acccgctgca taggtagcgt
    42061 cacggtcaaa ctcgtactcg ttccagttgg taagagtgaa tttctcgctg cccagcataa
    42121 agttgtaacc tgcaacccag ccggcgacat agccgttgtc gccagtgtaa taggtttgat
    42181 ctgtataacg cttagcaaag aacggtttga accaccaacc gctgccggtg aaattgtagc
    42241 cgataccgta caggaacata tcgtcgtgga aattcacgcg attcgcagaa ccgtaggtgc
    42301 catacgcatg caagtagaga ttaaatccgg tgtcacccag gtaaatacgg ttggtatttt
    42361 taaaggtata acgctgctcg ctacctggtt tattatggcg accgttatag aagttttccc
    42421 agtcgaagaa cccgtacatt tctccccaac taaagttagc gccaccttca aattcaagat
    42481 aaccaaaatc atctttgtgt gatttggtcg acgatttttc ggtagtccgg ctggtccagt
    42541 ccagataatg cacgccgata tctgcaaaac cgcctttaaa ttctgcatga gatacaacag
    42601 gtatcgctaa tatagaggaa agtgtcagag ttaaaagatg ttttttcata gcataattcc
    42661 ctatgccgat cgttattagt agattgctta ataaagtggt ttgctaaaaa cgacatattg
    42721 ttaatgcaaa ggaattaata tcgccaatac agtttttacc gtggattaat agagaaaaga
    42781 tataaatcca tcgatatcga atcgctattg aatgcgtgag aaaagtgtat tggtaagagc
    42841 cgggacaacc tcgctataaa aaggcgttag attccaccct acagaaaaag gatatgtatg
    42901 tctcaggttc aattttctca taacccgctg ttttgcatag atattatcaa gacttataaa
    42961 cctgatttca cgccacgagt ggcctttatt ttaggttccg ggctcggcgc gctggccgat
    43021 cagattgaga acgctgtcgc aatttcctac gaaaagctgc cgggcttccc ggtaagtacg
    43081 gtacatggtc atgcggggga gttagtgctg ggccatctcc agggcgtacc ggtggtatgt
    43141 atgaaaggtc gcggacattt ctacgaaggt cgtggaatga ccattatgac tgacgcaatt
    43201 cgtaccttta agctgctggg ctgcgagcta ctgttctgca ccaatgcggc aggctcgctg
    43261 cgtccggaag tgggggcagg cagcctggtc gcattgaaag atcatatcaa caccatgccc
    43321 ggtacgccga tggtgggtct taacgatgat cgttttggag agcgcttctt ctcactggca
    43381 aatgcctacg atgcggaata ccgcgcactg ttacaaaaag tggcgaaaga agagggtttc
    43441 cctctgacgg agggcgtgtt cgtctcgtat ccggggccga atttcgagac tgcggcggaa
    43501 attcgcatga tgcaaattat tggtggggat gttgttggta tgtctgtggt gcctgaggtt
    43561 atttcagctc gccattgcga ccttaaagtc gttgcggtct ctgcgattac caatatggcg
    43621 gaaggtttaa gcgatgtgaa attgtcacat gcgcaaacgc tggcggctgc ggagctctct
    43681 aagcagaact tcatcaacct tatttgtggc tttctgcgca aaattgcctg agaaaaacaa
    43741 taagaaacgg cctcgcgggt gaggccaaat gatgcgacaa ggaaacgatt atgagcatcg
    43801 cgatgcgctt aaaggtaatg tcctttttgc aatattttat ctgggggagc tggctggtta
    43861 ccctcggctc ttacatgatt aatacccttc atttcaccgg cgctaatgtt ggcatggttt
    43921 acagttccaa agggatcgcc gcgattatta tgcctggtat aatggggatc atcgcagaca
    43981 aatggctgcg cgcagaacgt gcatacatgc tgtgtcacct ggtgtgtgcg ggcgtacttt
    44041 tttatgcggc atccgtaact gatccggata tgatgttttg ggtgatgtta gtcaatgcga
    44101 tggcgtttat gccgactatt gcgttatcga acagcgtctc ttattcctgt cttgcccagg
    44161 cagggcttga cccggtgacc gctttcccgc ccattcgcgt ttttggtacg gtggggttca
    44221 ttgtcgcgat gtgggcagta agcctgctgc atctggaatt gagtagtctg cagctgtata
    44281 tcgcgtccgg tgcgtcattg ctgctgtcgg cttatgcgct gactttgccg aagattccgg
    44341 ttgcggagaa aaaagcgacc acatcgcttg ccagcaagct gggtctggat gccttcgtgc
    44401 tgtttaaaaa tccacgcatg gccatctttt tcctctttgc catgatgctg ggtgcggtac
    44461 tgcaaattac caacgttttt ggtaatccgt tcctacatga tttcgcccgt aacccggagt
    44521 ttgctgacag ttttgtggtg aaatatccct ccattttact gtcagtttca cagatggcag
    44581 aagtgggctt tatactgact atcccattct ttttaaagcg atttggcatt aaagccgtca
    44641 tgctgatgag tatggtggcc tggacgctgc gctttggctt cttcgcctat ggcgatccgt
    44701 caacaaccgg atttattttg ctgctgctgt cgatgattgt ttatggctgt gcattcgatt
    44761 tcttcaatat ttctggttcg gtatttgtcg aacaggaagt tgattccagc attcgtgcca
    44821 gcgcgcaggg gctctttatg accatggtaa atggtgtcgg cgcatgggtt ggctcgattc
    44881 tgagtggcat ggcagtagat tacttttcgg tggatggcgt aaaagactgg caaactatct
    44941 ggctggtgtt tgcaggatat gctctttttc tcgcagtgat atttttcttt gggtttaaat
    45001 ataatcatga ccctgaaaag ataaagcatc gagcggtggc tcattaaaag gattcgcagc
    45061 tctgctcttc agagctgctt ttatgataaa ggttaattag tgaaagatat tttattttct
    45121 tccggcgttg gttttggcat tggtgctttg tttacaatcg tgcgcttgcc tattcctgta
    45181 ccaaatgtat taccaggtat attatccatt gtgtttatgt atgtcggata tctggtggtg
    45241 aaatacttta tgccatgata atttaatacg atgtatttat tatatggagc acttaattat
    45301 ggaacgcgta tacagaacag atcttaagtt gctccgttat tttcttgccg tagcggaaga
    45361 gttgcatttt ggccgcgcag cagcgcgttt aaatatgtct cagcctccgc tcagtattca
    45421 tattaaagag ctggaaaatc aactcggcac gcagcttttt attcgccatt cgcgcagcgt
    45481 cgtactgaca cacgcgggca aaatcttgat ggaagaatcg cgtcgattgc tggtgaatgc
    45541 taataatgta ttggctcggg ttgaacaaat aggtcgggga gaagcagggc ggattgaact
    45601 cggcgtagtg ggaacggcta tgtggggacg gatgcgcccg gttatgcggc gattcctcag
    45661 ggaaaatcct aacgttgaag ttctttttcg cgaaaagatg cccgcgatgc aaatggccct
    45721 tctggaacga cgtgaactcg atgccgggat ctggcgaatg gcgacagaac caccgacagg
    45781 ttttaccagc ttacggttgc atgaatcggc gtttctggtg gcgatgcctg aagagcatca
    45841 tctctcatca ttttccaccg ttccgctgga agcgctacgt gacgagtatt ttgttacaat
    45901 gccgcccgtg tacactgact gggatttttt gcagcgagtt tgccagcagg tgggattttc
    45961 accggttgtt attcgcgaag ttaatgaacc gcaaacggta ctcgccatgg tcagtatggg
    46021 tattggtatc acattgatag cggacagcta cgcacaaatg aactggccag gtgtcatttt
    46081 ccgtccgctc aaacagcgca tccctgcaga tttatatatt gtttatgaaa cacagcaggt
    46141 gacgcccgcg atggttaagc tgttggctgc attgacacag taggtgagga gacaggaatt
    46201 gccggaaata gtcgtctgct aagtgaagcg catcaggcaa tttcgcaaat cgcagatagc
    46261 aaaaaagcgc ctttagggcg cttttttaca ttggtgggtc gtgcaggatt cgaacctgcg
    46321 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccgaa gtggtgggtg
    46381 atgacgggat cgaaccgccg accccctcct tgtaagggag gtgctctccc agctgagcta
    46441 atcacccact tcggtacttc acatttttac aagaaattta gctggcgaga aagtggtggg
    46501 tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg aggtgctctc ccagctgagc
    46561 taatcaccca cttctcaatt tcttgctaca cggcggagac tacataaagt agttggtggg
    46621 tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg aggtgctctc ccagctgagc
    46681 taatcacccc cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt
    46741 ttctaaagaa attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg
    46801 ttgcgcaatt tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg
    46861 aatcaggcgg gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg
    46921 gcatctttct aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca
    46981 acaggctatc tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt
    47041 aaccacggcg gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg
    47101 gaagctatcg aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt
    47161 ccgtactacc agaccaaacg ttttgatcgc tacaatgcgg tgatcgatca gatgctggaa
    47221 gagggcactg cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag
    47281 caaatggcga aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat
    47341 catgctgatg atgaaccgtg tgtcgtgcgt tttgctaatc cgcaggaagg ttctgttgtt
    47401 tttgacgatc agatccgtgg tccgatcgag ttcagcaacc aggagctgga tgatctgatt
    47461 atccgccgta ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat
    47521 atggaaatca cccacgttat ccgtggcgaa gaccatatca acaacacgcc gcgccagatc
    47581 aacattctta aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat
    47641 ggcgatgacg gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt
    47701 gatgacggtt atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccac
    47761 ggcgatcagg aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc
    47821 agcaaatctg ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt
    47881 aacgcgctgc cgccggagta tgttgctact cacttacagt ggcacattga gcaggaaaat
    47941 atcgataccc gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc
    48001 aagacgctga aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc
    48061 gatgccgatg cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt
    48121 cgtgacaaac tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag
    48181 gcgacggcgg atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc
    48241 gtaaccggtg cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc
    48301 cgcagtatcg agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag
    48361 taattagcgt gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc
    48421 tgattcgtca atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg
    48481 cgcgactttt tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata
    48541 cgccggggcg cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat
    48601 tggtgtgtta tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag
    48661 aataagtctg gcacgaccgc ctttcacgcc gggaaattta atgagatggt cactccctcc
    48721 ttcccagtac tatgctgagg acaggctttc attcggagaa ccatcatgga aaacattgcg
    48781 cttattggta tcgatctggg taagaactct ttccatattc attgtcagga tcatcgtggg
    48841 aaggccgttt accgtaaaaa attcacccga ccaaagctaa tcgaatttct ggcgacatgc
    48901 ccggcaacaa ccatcgcgat ggaagcctgt ggcggttctc actttatggc acgcaagctg
    48961 gaagagttag ggcattttcc aaagctgata tcaccgcaat ttgtccgccc attcgttaaa
    49021 agcaacaaaa atgacttcgt tgatgctgaa gctatctgtg aagcagcatc acgtccatct
    49081 atgcgtttcg tgcagcccag aaccgaatct cagcaggcaa tgcgagctct gcatcgtgtc
    49141 cgtgaatccc tggttcagga taaggtgaaa acaactaatc agatgcatgc ttttctgctg
    49201 gaatttggta tcagcgttcc gcgaggtgct gccgttatta gtcgactgag tacccttctt
    49261 gaggacagta gtttgcctct ttatctcagc cagttactgc tgaaattaca acagcattat
    49321 cactatcttg ttgagcagat taaagatctg gaatctcagt tgaaacgaaa gttggacgaa
    49381 gatgaggttg gacagcgctt gctgagtatt ccctgcgttg gaacgctgac tgccagtact
    49441 atttcaactg agattggcga cgggaagcag tacgccagca gccgtgactt tgcggcggca
    49501 acagggctgg taccccgaca gtacagcacg ggaggtcgga cgacattgtt agggattagc
    49561 aagcggggca acaaaaagat ccgaactttg ttggttcagt gtgccagggt attcatacaa
    49621 aaactggaac accagtctgg caagttggcc gactgggtca gggagttgtt gtgtcggaaa
    49681 agcaactttg tcgtcacctg tgctctggca aacaagctgg ccagaatagc ctgggcactg
    49741 acggcgcgac agcaaactta cgaagcataa aggcagaaat acaccagttt aaacaatcat
    49801 tcatctggtt ttgcgaatac tgatattgat gatactaacg gcccaccggc ctgttgagga
    49861 acctgtaaaa cggaaaggct cattgaagcc gtatattttc tggaggttca tcaggcgcgg
    49921 aactcatcga ggcgcgggaa taaaatccca ttcagacgcc ggatagattc aagcaagcca
    49981 acttgtcgtc aaaatcggtg ttgcaaaaac ggga
//
LOCUS       DAFNBJ010000013        48493 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000013 DAFNBJ010000000
VERSION     DAFNBJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.013"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            347..1564
                     /gene="alaA"
                     /locus_tag="L2X49_000773"
     CDS             347..1564
                     /gene="alaA"
                     /locus_tag="L2X49_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBN4795907.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1648..2247
                     /gene="yfbR"
                     /locus_tag="L2X49_000774"
     CDS             1648..2247
                     /gene="yfbR"
                     /locus_tag="L2X49_000774"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBN4795908.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     ATIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2306..4138)
                     /locus_tag="L2X49_000775"
     CDS             complement(2306..4138)
                     /locus_tag="L2X49_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBN4795909.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4225..4875)
                     /gene="hxpA"
                     /locus_tag="L2X49_000776"
     CDS             complement(4225..4875)
                     /gene="hxpA"
                     /locus_tag="L2X49_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBN4795910.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4886..5380)
                     /locus_tag="L2X49_000777"
     CDS             complement(4886..5380)
                     /locus_tag="L2X49_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBN4795911.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5463..5918)
                     /locus_tag="L2X49_000778"
     CDS             complement(5463..5918)
                     /locus_tag="L2X49_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBN4795912.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6256..7458
                     /gene="ackA"
                     /locus_tag="L2X49_000779"
     CDS             6256..7458
                     /gene="ackA"
                     /locus_tag="L2X49_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBN4795913.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7533..9677
                     /gene="pta"
                     /locus_tag="L2X49_000780"
     CDS             7533..9677
                     /gene="pta"
                     /locus_tag="L2X49_000780"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBN4795914.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9867..11387
                     /gene="yfcC"
                     /locus_tag="L2X49_000781"
     CDS             9867..11387
                     /gene="yfcC"
                     /locus_tag="L2X49_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBN4795915.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11420..11962)
                     /gene="yfcD"
                     /locus_tag="L2X49_000782"
     CDS             complement(11420..11962)
                     /gene="yfcD"
                     /locus_tag="L2X49_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBN4795916.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12020..12571)
                     /gene="yfcE"
                     /locus_tag="L2X49_000783"
     CDS             complement(12020..12571)
                     /gene="yfcE"
                     /locus_tag="L2X49_000783"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBN4795917.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12627..13271)
                     /gene="yfcF"
                     /locus_tag="L2X49_000784"
     CDS             complement(12627..13271)
                     /gene="yfcF"
                     /locus_tag="L2X49_000784"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBN4795918.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEVKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13407..14054
                     /gene="yfcG"
                     /locus_tag="L2X49_000785"
     CDS             13407..14054
                     /gene="yfcG"
                     /locus_tag="L2X49_000785"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBN4795919.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14111..14473
                     /gene="folX"
                     /locus_tag="L2X49_000786"
     CDS             14111..14473
                     /gene="folX"
                     /locus_tag="L2X49_000786"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBN4795920.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14494..15387
                     /locus_tag="L2X49_000787"
     CDS             14494..15387
                     /locus_tag="L2X49_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBN4795921.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEPKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15435..16325)
                     /gene="rpnB"
                     /locus_tag="L2X49_000788"
     CDS             complement(15435..16325)
                     /gene="rpnB"
                     /locus_tag="L2X49_000788"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBN4795922.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16522..17295)
                     /gene="hisP"
                     /locus_tag="L2X49_000789"
     CDS             complement(16522..17295)
                     /gene="hisP"
                     /locus_tag="L2X49_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBN4795923.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVSGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17303..18019)
                     /locus_tag="L2X49_000790"
     CDS             complement(17303..18019)
                     /locus_tag="L2X49_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4795924.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18016..18702)
                     /gene="hisQ"
                     /locus_tag="L2X49_000791"
     CDS             complement(18016..18702)
                     /gene="hisQ"
                     /locus_tag="L2X49_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBN4795925.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18792..19574)
                     /gene="hisJ"
                     /locus_tag="L2X49_000792"
     CDS             complement(18792..19574)
                     /gene="hisJ"
                     /locus_tag="L2X49_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBN4795926.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFIENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19795..20577)
                     /gene="argT"
                     /locus_tag="L2X49_000793"
     CDS             complement(19795..20577)
                     /gene="argT"
                     /locus_tag="L2X49_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBN4795927.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20843..21412)
                     /gene="ubiX"
                     /locus_tag="L2X49_000794"
     CDS             complement(20843..21412)
                     /gene="ubiX"
                     /locus_tag="L2X49_000794"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBN4795928.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21507..23024)
                     /gene="purF"
                     /locus_tag="L2X49_000795"
     CDS             complement(21507..23024)
                     /gene="purF"
                     /locus_tag="L2X49_000795"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBN4795929.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23061..23549)
                     /gene="cvpA"
                     /locus_tag="L2X49_000796"
     CDS             complement(23061..23549)
                     /gene="cvpA"
                     /locus_tag="L2X49_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBN4795930.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23808..24470)
                     /gene="dedD"
                     /locus_tag="L2X49_000797"
     CDS             complement(23808..24470)
                     /gene="dedD"
                     /locus_tag="L2X49_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBN4795931.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24460..25728)
                     /gene="folC"
                     /locus_tag="L2X49_000798"
     CDS             complement(24460..25728)
                     /gene="folC"
                     /locus_tag="L2X49_000798"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBN4795932.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25798..26712)
                     /gene="accD"
                     /locus_tag="L2X49_000799"
     CDS             complement(25798..26712)
                     /gene="accD"
                     /locus_tag="L2X49_000799"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBN4795933.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26868..27527)
                     /locus_tag="L2X49_000800"
     CDS             complement(26868..27527)
                     /locus_tag="L2X49_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBN4795934.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27610..28422)
                     /gene="truA"
                     /locus_tag="L2X49_000801"
     CDS             complement(27610..28422)
                     /gene="truA"
                     /locus_tag="L2X49_000801"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBN4795935.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28422..29435)
                     /locus_tag="L2X49_000802"
     CDS             complement(28422..29435)
                     /locus_tag="L2X49_000802"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBN4795936.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSCISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEACDVFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29501..30637)
                     /gene="pdxB"
                     /locus_tag="L2X49_000803"
     CDS             complement(29501..30637)
                     /gene="pdxB"
                     /locus_tag="L2X49_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBN4795937.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGNFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30736..31731
                     /gene="flk"
                     /locus_tag="L2X49_000804"
     CDS             30736..31731
                     /gene="flk"
                     /locus_tag="L2X49_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBN4795938.1"
                     /translation="MIQPISGPPPGQPPSQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31728..32906)
                     /locus_tag="L2X49_000805"
     CDS             complement(31728..32906)
                     /locus_tag="L2X49_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HBN4795939.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33190..34410)
                     /gene="fabB"
                     /locus_tag="L2X49_000806"
     CDS             complement(33190..34410)
                     /gene="fabB"
                     /locus_tag="L2X49_000806"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBN4795940.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34569..36575
                     /gene="mnmC"
                     /locus_tag="L2X49_000807"
     CDS             34569..36575
                     /gene="mnmC"
                     /locus_tag="L2X49_000807"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBN4795941.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTIPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36696..36974)
                     /locus_tag="L2X49_000808"
     CDS             complement(36696..36974)
                     /locus_tag="L2X49_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBN4795942.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37008..37556)
                     /gene="epmC"
                     /locus_tag="L2X49_000809"
     CDS             complement(37008..37556)
                     /gene="epmC"
                     /locus_tag="L2X49_000809"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBN4795943.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37556..38365)
                     /locus_tag="L2X49_000810"
     CDS             complement(37556..38365)
                     /locus_tag="L2X49_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBN4795944.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYTLAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38365..39189)
                     /gene="mepA"
                     /locus_tag="L2X49_000811"
     CDS             complement(38365..39189)
                     /gene="mepA"
                     /locus_tag="L2X49_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBN4795945.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39193..40278)
                     /gene="aroC"
                     /locus_tag="L2X49_000812"
     CDS             complement(39193..40278)
                     /gene="aroC"
                     /locus_tag="L2X49_000812"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBN4795946.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40313..41245)
                     /gene="prmB"
                     /locus_tag="L2X49_000813"
     CDS             complement(40313..41245)
                     /gene="prmB"
                     /locus_tag="L2X49_000813"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBN4795947.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41411..41962
                     /gene="smrB"
                     /locus_tag="L2X49_000814"
     CDS             41411..41962
                     /gene="smrB"
                     /locus_tag="L2X49_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBN4795948.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42084..42956)
                     /locus_tag="L2X49_000815"
     CDS             complement(42084..42956)
                     /locus_tag="L2X49_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBN4795949.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(42943..43467)
                     /locus_tag="L2X49_000816"
     CDS             complement(42943..43467)
                     /locus_tag="L2X49_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4795950.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43464..43934)
                     /locus_tag="L2X49_000817"
     CDS             complement(43464..43934)
                     /locus_tag="L2X49_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4795951.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(43931..44479)
                     /locus_tag="L2X49_000818"
     CDS             complement(43931..44479)
                     /locus_tag="L2X49_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4795952.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44454..45206)
                     /locus_tag="L2X49_000819"
     CDS             complement(44454..45206)
                     /locus_tag="L2X49_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBN4795953.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(45226..47871)
                     /gene="yfcU"
                     /locus_tag="L2X49_000820"
     CDS             complement(45226..47871)
                     /gene="yfcU"
                     /locus_tag="L2X49_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HBN4795954.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEEFSGDDTQKKWEWSRYYAWRALPSLKAKLALGEDYLNSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNMSVSLTGY
                     RYEYDNRADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDD
                     IKPVIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(47953..>48493)
                     /locus_tag="L2X49_000821"
     CDS             complement(47953..>48493)
                     /locus_tag="L2X49_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBN4795955.1"
                     /translation="IAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIVPDDHKL
                     EVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYYTIF
                     NTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAA
                     DAPDLGNFEANTTFQITYL"
BASE COUNT        12005 a        13054 c        11994 g        11440 t
ORIGIN      
        1 gacacctcgg aaatcatcac gctgttaaac tctccgtaat gcggaaatat catcatcgcg
       61 tttcattagt gaattcttaa atgaggcatt ttcacacaat tatcttacag ataaaaaaac
      121 cagacttaca attaagaatc agaacaattc actatataac attgcatgta aagcatatac
      181 acctcattat tttgtcatta ttaagttatt aacagcacaa tcgagccttc ccctctggca
      241 aaatcttatt ctgcagacct tcaaaacacc gtcctggggg agtacattgt tctaagctga
      301 cttccacggc agggagtggc gataacagca aaaaaggtca agattcatgt cccccattga
      361 aaaatccagc aaattagaga atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc
      421 aaaacgcctg gaagaagaag gtaacaaggt actgaaactg aacatcggca acccagcccc
      481 gttcggtttt gacgcgccag atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc
      541 tcaagggtat tgcgattcca aaggtcttta ctccgcgcgt aaagccatca tgcagcacta
      601 ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt tacatcggca atggtgtatc
      661 ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc
      721 tgcaccagat tacccactat ggaccgcggc ggtttcgctt tccagcggta aagcggtgca
      781 ttatctttgc gatgaatcct ctgactggtt cccggacctc gatgatattc gcgctaaaat
      841 tacgcctcgt acgcgtggga tcgttattat caacccaaat aacccaaccg gcgcggtata
      901 ttccaaagag cttttaatgg agattgtgga gattgcacgt cagcataatc tcattatctt
      961 cgccgatgaa atttatgaca aaattcttta cgacgacgct gagcatcact caattgcgcc
     1021 gctggcacct gacctgctga ccattacctt taacggactg tcgaaaacgt accgcgttgc
     1081 aggcttccgt caggggtgga tggtgttgaa cgggccgaaa aaacacgcca aaggctacat
     1141 cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc
     1201 cattcagacc gcgctgggtg gttatcagag catcagtgaa tttattaccc ctggcggtcg
     1261 tctttatgag cagcgtaacc gcgcgtggga actgatcaac gatattccgg gcgtttcctg
     1321 cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc gacgccaaac gctttaacat
     1381 tcacgacgat cagaaaatgg tgctggattt cctgttgcag gaaaaagttc tgttggtgca
     1441 agggacggca ttcaactggc cgtggccgga tcacttccgc attgtcacgc taccgcgtgt
     1501 cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct
     1561 gtaatcttaa tttcactgcc ggagattgca tccggcagcg ttatcccgcc acaatgacct
     1621 gatgatgtca tcatacgtaa ggtcactatg aaacagagcc atttttttgc ccatctctcc
     1681 cgcctgaaac tcattaaccg ctggccgctc atgcgcaacg tgcggacgga aaatgtgtcc
     1741 gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg caactatcaa aaatcggaaa
     1801 tttggcggta atgtcaacgc cgaacgtatc gctttactgg cgatgtacca cgatgcctca
     1861 gaagtgctca ccggcgatct ccctactccg gtgaaatact tcaattcgca aatcgctcag
     1921 gagtacaagg ctattgaaaa aatcgctcag caaaaactgg tcgatatggt tccggaagag
     1981 ctgcgggata tctttgcgcc gttaattgac gagcatgcat atagcgatga agaaaaatcg
     2041 ctggtgaaac aggcagatgc actgtgtgca tatctgaaat gtctggaaga actcgcggcc
     2101 ggaaataatg aattcttgct ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc
     2161 agccaggaga tggactactt catggaagta tttgttccca gcttccatct ttcgctcgat
     2221 gagattagcc aggattcacc gctgtaagca gccggagtct gcgtcgcatc aggcaataag
     2281 cgccggatgc gacatcaggc tcttgtcaaa acggaaacag catcgggatc atcaccacac
     2341 aaaccgccat cacgataatg gtgaacggta cccccaactt cacaaagtca ctgaagctgt
     2401 aatttcccgg acctaaaacc agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg
     2461 cggatgctgc catcgccacg accatcgcga atggataagg cgacaccccc atcgttttgg
     2521 cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc ggtattagag ataaatagcc
     2581 caataaccgc cgacaagaca aacaaacaac ccagcatcat atgtggcccg taaccaccgc
     2641 caatgtccat cagccctttc accgccagcg cgacacctcc cgttttctgt aatgccacag
     2701 caaacggcat catcccaacg atcaaaataa tgctcggcca gtgaatggat ttataggcgc
     2761 tttcagcatc tatacagcgg aatttcccca tcagcaggca ggcgatgata gcggcaacag
     2821 gattaggaat ttcatctgtc agcattaacg ccaccattag caccagacag aaaatggcat
     2881 ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc ttccggtaag ttcagcgcta
     2941 cgaagtcgcg gccctgtttg gccagcatac cgatcagttt ccagttaccc acaaccagga
     3001 tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc ttccagcgcc acgccattgc
     3061 gctttagccc caccacattc agtccgtagc gggtacgaaa accaatttcg cgcaccgatt
     3121 taccaatcag ttctgactca ggaattaatg aaatctctgc catgcccaca tcaagggcct
     3181 ggtcagaaaa atactcgccg cgcagtacca tcggctccag caattgctca ctacaaaatt
     3241 gccggagatc gacatcagcc gcagacatat caataagcaa aacgtcacgc gcgcgaaatt
     3301 cagaaacccc attaacgttc acgataacgc gacgaaaacg ccgccagcgt tcaacaccga
     3361 tgacgttagc gccataacgc tcacgtaatt tgagatcatc cagccgttga cctatcattg
     3421 gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt cagtcgatat tcacggataa
     3481 gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg ggtctgggta tcccctttca
     3541 gcatgaaacg catcactaac atatacaaaa tacccagcac cagcacgacc aggccaatag
     3601 gtgttacgct aaagaaactg aagccgtgat agccttcacg cagcaattca ctgttgacta
     3661 ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct tatcagcccg gcaaaactca
     3721 acggcatcat cagacgtgac ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg
     3781 gaataaagat agcgacaacg ccggttgaac tcataaacgc gccaagcccc gcgacggtca
     3841 gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc aactttgacc agccatgttc
     3901 ccattacggt ggcaacaccg gtacggacca aaccatcgcc aataataaac aaggcggcaa
     3961 tcaggacaac gttaggatca gaaaagccgg aaaatacttc ggggactgtc agcgttccgc
     4021 ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc catacgcact ctgcccgtcg
     4081 caaacaagac aatggcaacg gccagtaatg aaagaaccca aatcaattca ccgttcacaa
     4141 cttatccttg ttaattgagg gagatgtctt gattctgcca taaaaaagcc ccgacgagac
     4201 ggggctaaat catgatcagg tgtttcactg aataataaca tcgccatttg gctgtttggt
     4261 cacagtaatt tgctccagac tgtggaggac caaatcgacc tcattcaggc gcggggtatc
     4321 tgccggagcg ttaaccgcaa tgacatggca acccgccgcc aggccagaaa gcacgccagc
     4381 gggagcatct tccaccacca cacactcctg cggcgcaagc cccagcagct gcgcacctaa
     4441 cagatacgca tcaggttctg gttttccgcg cttcactcgc tcagcggtta caaacacctc
     4501 tggtgcggga agcccagcta ttttatggcg cgctcgcgct accggcatgg agccagaagt
     4561 cacaatggcc cacggaatac ctgctttatt caaatgactg agtaaggcga ttgcccccgg
     4621 aagcgcggta ataccttcgg tttccgtggc ctcgatttgc tccagacgcg taaactcggc
     4681 ggcaatatca gcctcggatt tgcccgccat aaaatggcgc agagaggtga tcgcctgttt
     4741 accgtgaatg aaagccagca cctcttccgg cgctaaccca tgacgtctgg cccagttgct
     4801 ccacgcccgt tctaccgcag gcagggaatc caccagcgtt ccatcaagat caaacagaaa
     4861 acctttgcac cgcacgcggg cctcctcagg cgttgataat ttgattaatt tcgttggcgc
     4921 tcaaatggta ctgacgcggg caggcatgcc acacattaag catgcgctga tatttttccc
     4981 acattggcgt ctgggcgtta aaaccgtgag ttccggcgtc aaaatgggta tagcgccctt
     5041 ccacattaac cataaagcgg acataaccga ggtaacgtgc ttcagtggcg gcgtcaaagc
     5101 cgaggaaggt gacacgacgt tcatcgatgg attgctggtc ctgcaaatta gaccaggaaa
     5161 catgcaacgc atgatacatc tccataatgt cgatgatagt gcggcaggtt tcttctttca
     5221 gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc gtaaccacgc tcaataattg
     5281 tttgcaggcg acggtaacgt tcagcatttg ccggatcgag catagtcatc atcttgtact
     5341 gattagacaa aataagacgt tgcgcgttgg tcatttccat tgttgactcc tgtatcacta
     5401 tactacggtg aaaaaaaaga aggctgagta tgccttcttt tatatgcgta atcaggggtc
     5461 aattacaaat catcaaggaa agttttatcc agttgtttga aggcgcgctt aagcgtgtca
     5521 gctaatgcct ggtaatcagg cttgccttca acgggtgcca acacctgtcc agactcctgc
     5581 aatttaccgc gaacttcata aaaccagtta aggattgcag ggggtaatgg cgttacagaa
     5641 cgcttgccca gccaccacaa tccctgcatg ggtaaactta aggcgaacag cgcagtggca
     5701 actgccggcc caagctgacc gcccagggca atctgccagc agagagtaaa tacggcgatc
     5761 ggcggcataa aacggatcgc ataacgcgtc atcttgataa cgcgattttc gacaaagacc
     5821 ggggcaaggc gtttttccag tggccacgtc tttgagtaat gctgtccgcg gcgaaacaag
     5881 ctaaaaaaat taacagaacg attatccggc gttgacatgc ttcacctcaa cttcacatat
     5941 aaagattcaa aaatttgtgc aaattcacaa ctcagcggga caacgttcaa aacattttgt
     6001 cttccatacc cactatcagg tatcctttag cagcctgaag gcctaagtag tacatattca
     6061 ttgagtcgtc aaattcatat acattatgcc attggctgaa aattacgcaa aatggcatag
     6121 actcaagata tttcttccat catgcaaaaa aaatttgcag tgcatgatgt taatcataaa
     6181 tgtcggtgtc atcatgcgct acgctctatg gctcactgac gtttttttag ccacgtatca
     6241 attataggta cttccatgtc gagtaagtta gtactggttc tgaactgcgg tagttcttca
     6301 ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt acctttctgg tttagccgaa
     6361 tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg acggtaataa acaggaagcg
     6421 gctttaggtg caggcgccgc tcacagcgaa gcgctcaact ttatcgttaa tactattctg
     6481 gcacaaaaac cagaactgtc tgcgcagctg actgctatcg gtcaccgtat cgtacacggc
     6541 ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg ttattcaggg tatcaaagat
     6601 gcagcttctt ttgcaccgct gcacaacccg gctcacctga tcggtatcga agaagctctg
     6661 aaatctttcc cacagctgaa agacaaaaac gttgctgtat ttgacaccgc gttccaccag
     6721 actatgccgg aagagtctta cctctacgcc ctgccttaca acctgtacaa agagcacggc
     6781 atccgtcgtt acggcgcgca cggcaccagc cacttctatg taacccagga agcggcaaaa
     6841 atgctgaaca aaccggtaga agaactgaac atcatcacct gccacctggg caacggtggt
     6901 tccgtttctg ctatccgcaa cggtaaatgc gttgacacct ctatgggcct gaccccgctg
     6961 gaaggtctgg tcatgggtac ccgttctggt gatatcgatc cggcgatcat cttccacctg
     7021 cacgacaccc tgggcatgag cgttgacgca atcaacaaac tgctgaccaa agagtctggc
     7081 ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg ttgaagacaa ctacgcgacg
     7141 aaagaagacg cgaagcgcgc aatggacgtt tactgccacc gcctggcgaa atacatcggt
     7201 gcctacactg cgctgatgga tggtcgtctg gacgctgttg tattcactgg tggtatcggt
     7261 gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac tgggcgtgct gggctttgaa
     7321 gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat ctggtttcat caacaaagaa
     7381 ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac tggttatcgc gcaagacgcg
     7441 agccgcctga ctgcctgatt tcacaccgcc agctcagctg gcggtgctgt tttgtaaccc
     7501 gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg tattattatg ctgatcccta
     7561 ccggaaccag cgtcggtctg accagcgtca gccttggcgt gatccgtgca atggaacgca
     7621 aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc gcgtaccggt ggcgatgcgc
     7681 ccgatcagac tacgactatc gtgcgtgcga actcttccac cacgacggcc gctgaaccgc
     7741 tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca gaaagatgtg ctgatggaag
     7801 agatcgtcgc aaactaccac gctaacacca aagacgctga agtcgttctg gttgaaggtc
     7861 tggtcccgac acgtaagcac cagtttgccc agtctctgaa ctacgaaatc gctaaaacgc
     7921 tgaatgcgga aatcgtcttc gttatgtctc agggcactga caccccggaa cagctgaaag
     7981 agcgtatcga actgacccgc aacagcttcg gcggtgccaa aaacaccaac atcaccggcg
     8041 ttatcgttaa caaactgaac gcaccggttg atgaacaggg tcgtactcgc ccggatctgt
     8101 ccgagatttt cgacgactct tccaaagcta aagtaaacaa tgttgatccg gcgaagctgc
     8161 aagaatccag cccgctgccg gttctcggcg ctgtgccgtg gagctttgac ctgatcgcga
     8221 ctcgtgcgat cgatatggct cgccacctga atgcgaccat catcaacgaa ggcgacatca
     8281 atactcgccg cgttaaatcc gtcactttct gcgcacgcag cattccgcac atgctggagc
     8341 acttccgtgc cggttctctg ctggtgactt ccgcagaccg tcctgacgtg ctggtggccg
     8401 cttgcctggc agccatgaac ggcgtagaaa tcggtgccct gctgctgact ggcggctacg
     8461 aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt cgctaccggc ctgccggtat
     8521 ttatggtgaa caccaacacc tggcagacct ctctgagcct gcagagcttc aacctggaag
     8581 ttccggttga cgatcacgaa cgtatcgaga aagttcagga atacgttgct aactacatca
     8641 acgctgactg gatcgaatct ctgactgcca cttctgagcg cagccgtcgt ctgtctccgc
     8701 ctgcgttccg ttatcagctg actgaacttg cgcgcaaagc gggcaaacgt atcgtactgc
     8761 cggaaggtga cgaaccgcgt accgttaaag cagccgctat ctgtgctgaa cgtggtatcg
     8821 caacttgcgt actgctgggt aatccggcag agatcaaccg tgttgcagcg tctcagggtg
     8881 tagaactggg tgcagggatt gaaatcgttg atccagaagt ggttcgcgaa agctatgttg
     8941 gtcgtctggt cgaactgcgt aagaacaaag gcatgaccga aaccgttgcc cgcgaacagc
     9001 tggaagacaa cgtggtgctc ggtacgctga tgctggaaca ggatgaagtt gatggtctgg
     9061 tttccggtgc tgttcacact accgcaaaca ccatccgtcc gccgctgcag ctgatcaaaa
     9121 ctgcaccggg cagctccctg gtatcttccg tgttcttcat gctgctgccg gaacaggttt
     9181 acgtttacgg tgactgtgcg atcaacccgg atccgaccgc tgaacagctg gcagaaatcg
     9241 cgattcagtc cgctgattcc gctgcggcct tcggtatcga accgcgcgtt gctatgctct
     9301 cctactccac cggtacttct ggtgcaggta gcgacgtaga aaaagttcgc gaagcaactc
     9361 gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg tccgctgcag tacgacgctg
     9421 cggtaatggc tgacgttgcg aaatccaaag cgccgaactc tccggttgca ggtcgcgcta
     9481 ccgtgttcat cttcccggat ctgaacaccg gtaacaccac ctacaaagcg gtacagcgtt
     9541 ctgccgacct gatctccatc gggccgatgc tgcagggtat gcgcaagccg gttaacgacc
     9601 tgtcccgtgg cgcactggtt gacgatatcg tctacaccat cgcgctgact gcgattcagt
     9661 ctgcacagca gcagtaatct cgtcatcatc cgcagctttg cgctgcggat atctgaaccg
     9721 gaaataatca ctatttccgg ttttttattc tcttaatctg cattaatcct ttctgattat
     9781 cttgcttaac tgcgctgcat caatgaattg cgccatttca ctttgcatac ttaccacttt
     9841 gttttgtgca agggaatatt tgcgctatgt ccgcaatcac tgaatccaaa ccaacaagaa
     9901 gatgggcaat gcccgatacg ttggtgatta tcttttttgt tgctatttta accagccttg
     9961 ccacctgggt agttccggtg gggatgtttg acagtcagga agtgcagtat caggttgatg
    10021 gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt tcgcattctg actaacgaag
    10081 caggcgaacc tgagtatcac cgcgtacagc tgttcacgac gggcgatgaa cgcccgggcc
    10141 tgatgaactt cccgtttgaa ggattaacct caggatcgaa atacgggaca gctgttggca
    10201 tcatcatgtt tatgctggtg attggcggcg cgtttggcat tgtgatgcgt acaggaacca
    10261 ttgataacgg tatcctggcg cttattcgcc atacccgcgg gaatgaaatt ctctttattc
    10321 ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt tggtatggga gaagaggccg
    10381 tcgcctttgc cattatcatc gcaccgctaa tggtccggct gggctatgac agtattacca
    10441 ccgtcctggt gacctatatt gccacgcaaa tcggttttgc cagttcgtgg atgaacccgt
    10501 tttgtgtggt cgttgctcag gggattgccg gcgttccggt gctttctggc tccgggttgc
    10561 gcatcgtggt gtgggttatc gccactctga ttggcctgat ctttaccatg gtgtacgcct
    10621 cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga gtccgaccgc ttctttcgtg
    10681 aaaagcaggc tgatgttgaa caacgtccgt ttacctttgg tgactggctg gtattgattg
    10741 tcctgaccgc cgtaatggtc tgggtgattt ggggcgtgat cgttaatgcc tggtttattc
    10801 cagaaattgc cagccagttc ttcaccatgg gtctggtgat tggcatcatc ggcgttgttt
    10861 tccgccttaa cggcatgacg gttaatacca tggcttcatc ctttaccgaa ggggcgcgaa
    10921 tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg gattttgctg ctggtcggta
    10981 atggtgaagc aggtgatgcc agcgtgttaa ataccatcct caacagcatt gccaatgcca
    11041 ttagcggtct ggacaacgct gtcgcggcct ggtttatgtt gctcttccag gcggtattta
    11101 atttctttgt gacatccggt tctggtcagg cggcgttaac catgccgtta ctggcaccgc
    11161 ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct ggcattccag tttggtgatg
    11221 gcttcagcca catcatttac ccaacctcag cttcgttaat ggcgacgctc ggtgtttgcc
    11281 gggtggactt ccgtaactgg ctgaaggtgg gcgcgacact gcttggactg ctgtttatta
    11341 tgtccagcgt cgtggtgatc ggcgctcagt tgatgggcta ccactaaaaa tgttaagagc
    11401 cgcaattgcg gctctttttt cattctgccg tttcagtctc tacggcttca tttttggcat
    11461 tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg agtgaactca tcgcagcgtg
    11521 cggtgatttc ttccggcgtc agccagcaaa cttcactgac ttcatcttcc tgtagggcga
    11581 agggaccgtg agagacgcag ctgaacaatg cgccccagac acggcaattt ttatcttcga
    11641 aatagaactg cccgtgctca gcaaagggga caccggcaat gcccaactct tcttccgctt
    11701 cgcgacgcgc ggattccagc agttgctcat cggcctggac cacaccgcct gcggtcgcat
    11761 ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg ctggaccaga attttgccca
    11821 tgccatcatg cacgacgatg taagttgcac gatgacgcag acactgtgcc cgcatttgtt
    11881 cccggctggc ttgtgcaatg acttcgttct cttcattgac aatatccacc cattcagtac
    11941 ttgccaaacg acgctgttcc attatcagga aaccttcttt ttctggcgct cttacggcgc
    12001 attttgagtt gtgggtaaat tacggattaa tcgcgacctg cgcaatgata ctttgatcat
    12061 tgagtgcgat tacactaagt acgtcattat ccagcatgcc ataactcgcc ggattaccgc
    12121 ctttcggaat actcaccgag ccggggttga agtggaaaat ctccccccgc tgttctgcca
    12181 ctggcagatg ggtatgaccg tacaccagca catcgttctg gtttaaagca ggtagatttt
    12241 ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg tttttccagt aatacctgtt
    12301 gccacggcgc ggttatcggg aaatgcagca gcatttgatc cacttcgctg tcgcagttgc
    12361 cgcgcacagc gataacctta tgtgccactt cattaagccg ttcagcgact ttggctggcg
    12421 cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa cacgtcgcca aggatcacca
    12481 gccactgggc accgctttgg gcaaacaact ccagaacacg ttccgtcgcc ggtaacgacc
    12541 catgaatgtc cgatgcaaac atcagtttca tcactcactc ctcgtcgaaa aaacgtaagc
    12601 tatgatactg gattctgtcg ttgctatcag ccagattgct tcgccgaaag tgcaataaaa
    12661 cgctggacag acgctcgctg ccactggaat gtcgcataat ccaccaggcg ttctggcacc
    12721 tcatcgccat gtagtaccag gcggttaatc attagcgcca gatcagtatc agcaatgcac
    12781 cattcaccaa ataaattcgg ctgacccagt gctaacaaat gttctgccat cgcgaacagt
    12841 ttctctgcac tggcttttac ctcggccgtt agtggcgctt ttttcgcccc cgcaaagaca
    12901 acatccgtcg gacgctcttc gcggatgggc atcagatcgc tgcgcagcca ggcctgaatc
    12961 tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa tacgttccca ggtcggtggc
    13021 gcaaatcgat cttccagata ctccgcaatg gcagaagatt cactcaactc aaaatcatcg
    13081 atttgtaata acggcacacg gcgtgtctga ccgtaacctt gccacgtcgg ctgcaaatgt
    13141 tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca ggcctttttc ctgcaacgcc
    13201 acccaggcgg ataacacata aggggagaaa aagtgggcat ctgaccaaag cgtgatagcg
    13261 ggtttactca ttatgtcctc ggcaataaag ggggctggct atcaaactat agagcctttt
    13321 tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg ctttctatac ttgtggtgtt
    13381 cagatgatta aacaaccgga gctgcaatga tcgatctcta tttcgccccg acacccaatg
    13441 gtcacaaaat tacgctgttt ctcgaagaag cagagcttga ttatcgcttg attaaggtag
    13501 acctggggaa aggcggtcag tttcgtccgg aatttttgcg catttcgccc aacaacaaaa
    13561 ttccggcaat tgttgatcat tctcctgccg atggcggcga accgttaagc ctctttgagt
    13621 ctggtgccat tttgttgtat ctggctgaga aaacaggact ctttttgagt catgaaacgc
    13681 gtgagcgcgc cgccacatta cagtggttat tctggcaggt aggcggactg gggccgatgc
    13741 ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac cattccttac gctattgaac
    13801 gttatcaggt tgaaactcag cgtctttacc atgtactgaa caagcggctg gaaaactcgc
    13861 cctggctggg aggcgagaac tacagcattg cggatattgc ctgctggccg tgggttaatg
    13921 cctggactcg ccagcgaatt gacctcgcaa tgtatccggc agtcaagaac tggcatgagc
    13981 ggatccgttc gcgccctgcc accgggcagg cactgctaaa agcacaactc ggtgatgagc
    14041 gttcggatag ttaacagaaa caggttctcg tgtattattt catcctaagt aaaacaacgg
    14101 agaacctgca atggcacaac ctgccgctat tattcgtata aagaaccttc gtttacgtac
    14161 gtttatcgga attaaggaag aagaaattaa caaccgtcag gatattgtta tcaatgtgac
    14221 gatccactac cccgccgata aagcgcgcac tagcgaagat atcaacgatg cgctgaatta
    14281 tcgcaccgta acgaaaaaca ttattcagca tgtagagaat aaccgtttct ctttgttgga
    14341 aaaattaact caggatgtgc tcgatatcgc acgtgaacat cactgggtga cgtatgctga
    14401 agtggagatc gataaactgc acgcgctgcg ctacgccgat tcggtatcca tgaccttaag
    14461 ctggcagcgt taaccgccat atcgggaggc tgcatgaaca tagtgatcac cggagggacg
    14521 ggattaattg gtcgccattt gattccgcgt ttgctggagc tgggccatca aatcacggta
    14581 gtgacgcgta acccacagaa agccagttcc gttctcggcc ctcgggtgac gctatggcaa
    14641 gggctagccg atcaaagcaa cctcaacggc gttgatgcgg taatcaacct ggccggagaa
    14701 ccgattgctg ataaacgctg gactcacgag ccaaaagagc gtctctgcca aagccgctgg
    14761 aatatcacgc aaaaactggt cgatttgatt aatgccagcg acacgccgcc gtcggtactc
    14821 atttccggct cggcaacggg ctattatggc gacttaggtg aagtggtggt taccgaagag
    14881 gaaccgccgc ataacgaatt tactcataaa ctctgcgccc gctgggaaga gattgcctgc
    14941 cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa ccggcgtggt actggcaccg
    15001 gatggcggta ttctcggtaa aatgctgccg ccgtttcgtc ttggcctggg cgggccgatt
    15061 ggctctggtc ggcagtatct ggcctggatt catattgatg atatggtcaa cggtattctc
    15121 tggctgctgg ataacgagct gcgcgggcca tttaatatgg tttcgcccta cccggtacgc
    15181 aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc gcccggccat tttgcgcgtc
    15241 cccgcgaccg ccattcgact gttaatgggc gaatcttcag tactggtatt aggtggacaa
    15301 cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt ttcgctggta cgatttagaa
    15361 gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc aaacatccgc cagttaactc
    15421 ccggtgttac aggattagtg gctttgcgct ataagatcgt ctggcgatag tcgggtcacc
    15481 atcataacta actctctgtc taaacctcta tccagcatct cctgagcaat acgcagggct
    15541 tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc tgtcagcaat ggtcatcagt
    15601 ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa taaatgcacg gaaacgctgg
    15661 gcatcccctg tttgtaatac gtaattaaac agggctttta gctgtctgtc attagtgttc
    15721 cctgtaacta gcagcgaaac aatttggtcg actaatccca acagatcgcg ctgacgaata
    15781 tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt gcataatctc gtcatccggc
    15841 accacggtaa tatccaccaa cggaaacgct gatgaatata ttttgcgggc tatagcaggc
    15901 tcggcaaatt catcaagcca gcagagtgaa taaggataag gacttctgca accatgataa
    15961 aacagcatcg ggatcaccaa tggaagctct ttatagcccg catcaagatg gttttgcatt
    16021 gccgcaatgg aataacgcat catgcgaaaa gccattaatt cttccggctt actttggtgc
    16081 tctatcacta cataaatata acccactccc tcctgcgttt tcacagacca caagaggtcg
    16141 gaataatatt gccgcaggtc gtcatcaata aaactgtttg gttccagttt aagcgtcgtt
    16201 aaatcacaca gtttgcgcag cggcgcggga agatgaatat caataaaatc ccgcgcggtg
    16261 tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg gcgtggaagt tgtcgatatt
    16321 gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg acaataacca cgattaacac
    16381 ccaatgcagc aggcagtttt tatctttgga cagcgaattc agaagtattt ttagcggact
    16441 gcaactttta catttcagcg gaagctgact cgcaaattcc ccatgaaaat actccggcag
    16501 cgttagccac cggagaggaa attatttcag cgatccctta aggaactgtt gcagacgagg
    16561 gctttgcggg ttgccaaata actgctccgg cgcgccctct tcttctattt tcccctgatg
    16621 gaggaaaatg acgtgagtag aaacatggcg agcaaagccc atttcgtgag tcactaccac
    16681 catggttttc ccctcttctg ccagttgctg cataatacgt aacacttcgc ctaccagttc
    16741 aggatcgagc gccgaggtag gttcatcaaa cagtaaaact tccggttcca tcgccagcgc
    16801 ccgcgcgata gaaacacgct gttgctgacc gccggaaaga tgcaccggat atttcccctg
    16861 cgcacgttcg tctatcccga ctttcgccag atacttcacc gcccgctcgc gcgcttcctg
    16921 cttgctcagg cccaacacct gaatcggcgc ttccatgacg ttttccagca ccgtcatatg
    16981 gctccagaga ttgaagtgct ggaataccat cgtcaggcgt gtgcgcagta agcgcagttg
    17041 atttttatcg gcgactttga gttgaccatc tttgtcgcgc accagattga tcgtctggcc
    17101 actgaccacg atcgatcctt cgctcggttt ttcgaggaag ttaatgcagc gcagaaaggt
    17161 acttttcccc gatcccgacg atccgatgat gcttattaca tctccggcat tcgcttgcag
    17221 tgacacccct ttcagcactt cgtgttcgcc atagcgtttg tgcaaatcga taacgtttaa
    17281 tttattctcg gacatcgtat tctcagtgcg ttgaagaagg tttcacatgc tgcaaccagc
    17341 gtttttccgc tctgcgaaag aggctgatca ggacataaga gatgattaaa tagagcaccg
    17401 cggcaatgcc gaaggcggta aaaggttgat acgtggcggc gttaatatcg cgggctattt
    17461 tcagcagatc cggcaccgtg gccgtaaacg ccagcgcagt agagtgcagc atcaggatca
    17521 cttcattgct gtatgccggt aacgcaatac gcagcgcaga aggcaaaata atgcagcgat
    17581 acattttaaa ggtcgagaag ccataggctc tggcggcttc aatttcccca tgcggaaccg
    17641 aacggattgc cccagcaaaa atctcggtag tgtaagcgca ggtgttaagc gtcagcgcca
    17701 gcacggtaca gttcaggcca ctgcggaaga aagcgttaag gaattcggtt cccttaacaa
    17761 tctcaagcgt gtacatgccg gaatagaaca ccagcaactg aacatacagc ggcgtaccgc
    17821 gaaaaatata ggtaaataac cagattggaa actggatgta tttattactg gagacacgac
    17881 caatcgccag aaacagcgcc aggactccgc ctatcactac cgacaaaata agcagccaca
    17941 gagtgatcgc cacaccagta aagcgataac cgtcggtcca cagcagcggt ttccagtatt
    18001 catgtaagat ttcgatcaca ggtcagccct cttcacaccc acggagtagc ggcgctcaag
    18061 gaacagcagc acaccattgg aaacagtggt gaaaaccaag taaatcacgc cacagacgat
    18121 ggcgaaatag aacggttccc aggtactttt gcctgccagt tgggtggctt tgaccacatc
    18181 ttccaggccg agtaacgaaa ccagtgcggt agatttgagg atcacctgcc agttgttgcc
    18241 aatgcctggc agggcgtaac gcatcatcgc cggaaacatg atccgccgaa acacttgccc
    18301 acgagtaaaa ccgaacgccg tcgccgcctc tatatgtcct ttcggcactg ccataaaagc
    18361 accacggaac gtttcggtaa aataagcacc gtagataaaa ccgagagtga taataccagc
    18421 gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc tccgtcaccg tgtttagcgc
    18481 aatctgcaaa ccgtagaaaa tcagcagcat caacactaag tccggcacgc cacgaatcag
    18541 cgtggtgtac ccttcgaaaa tcagccccga aagccgattt tgcgagagct taccgccagc
    18601 gccaattaaa ccgatgatta cagcgagcac tacagagctg atagccagct ccagcgtgac
    18661 gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac atgctgcctg tcctgtcgtg
    18721 tgtggtgaat taccgcctct tgtctccctc cggttacccg aagggagcgg gctttctttc
    18781 accgatggcg attagccacc ataaacatca aaatcgaagt actttttcgc taatttctcg
    18841 taagtaccgt cagcgcgcat ttcggcaaag gctttgttca gtgcttcgcg cagttcgtta
    18901 tcttctttac gcaggcccat gccggtccct acgccaaaca gtttttcatc tttaacagac
    18961 gggccaccga atttgtaatc tttaccgaca ggttgtttga ggaaaccttc gctggcagcg
    19021 acctcatcct ggaacgcggc atcaatacgt ccggcagtca ggtcagaata aatgttgtcc
    19081 tgcccctgat acgagacgat ttcaatgcct tttggtgccc aatgttcatt accgaacgtc
    19141 tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt tcagcgactc gactgtcggc
    19201 tgaatgtcag aatttttcgc caccaccaaa cgagaatcgg cagcgtacag tttgtcggtg
    19261 aaggctattt cttgctgacg tttttccgta atggaaagcg atgacatgat ggcgtcaatc
    19321 ttcttcgctt ttaaggacgg gattaacgca tccagcggat tttcgataaa cgtacattgc
    19381 gtattgatgc gtttgcataa ttcctttgcc agatcgatat cgaagccaac cagttcgcct
    19441 tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg taccgatgcg gatgttttgc
    19501 ggaatcgcag caaacgccgc agttgcgctg gagaaggcca gaaccagaga gagcgatagc
    19561 accagttttt tcataatgtc ctcaactgac agtcttttta tagggaatat ttacaggttt
    19621 gttacactta tcgtgccata aaattgacca tcaaggcaaa atatcccgcc ccgttagacg
    19681 tattattttt cattaaaaat gcttaagtat gcatttcaat gcaccacgat agtgcaacaa
    19741 acctggcgtg caccatgatg atcattccat caggtacagc ttcccagcga cgtatcagtc
    19801 accgtagaca ttaaagtcga aatacttttt cgccatcttg tcgtaggtgc cgtcctgacg
    19861 cagctcgcca agcgccttat tgaaggcagc cgtcagttca gcatcatctt tacgtagccc
    19921 tacaccggtg ccatcaccga agtatttttt gtcttttact gatgagccag caaaggcgaa
    19981 atctttacca gcaggttgct tgaggaatcc ttcgctggca gcaacttcat cttgtaacgc
    20041 agcatccaga cgtcctgcag ccagatcgga atagaccaaa tcctggttgg cataggccac
    20101 cacatccacg cctttgctac gccaggtctc gttagcgtaa gcttcctggg ttgatccctg
    20161 cagcacacca acatgtttac ctttcagtga atccagcgtt ggctgaatcg gtgaaccttt
    20221 ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg gagaaggcaa tctcctgctg
    20281 acgtttatcg gtaatggaaa gcgacgaaat aatggcgtcg atttttttcg ctttcagtga
    20341 ggggatcagc gcgtcaaagt cactggcaac ccaggtacat ttcacctgca tccgtttgca
    20401 catctcgtta ccgagatcga tatcaaagcc aacaaaatca cctttagcat ctttcgacga
    20461 gaacggcgca taggtggtat cggttccgat acgtaccgtc tccggtagcg ccgcatagct
    20521 ggaagccgct gcggagagac cgactaacaa agacagagcg agaatcgact tcttcataca
    20581 taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg tgttgtgtgt ttgcaggctc
    20641 tttcatgcag gtcttatgcc atcttgacga caacagtaac attcaacgtt aaatatgtta
    20701 ataagacgtt gcattattgt cctgaagttg aagatagcag gtatagcggt tgaatcgcag
    20761 cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg atcgcggttg caaaattgcc
    20821 ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg ggcaaagaga tcttcaggaa
    20881 gggttatcgc aaactggtca agaacacgat taaccgtctg atttatcaca tcatcaaggg
    20941 attgcgggcg atgataaaac gccggaacgg gaggcataat caccgcaccg atttctgccg
    21001 cctgagtcat taaacgcaga tggcctaagt gcaatggtgt ttcacgcacg cagagcacca
    21061 acgggcgacg ctctttcagc accacatctg ccgcacgggt cagtaagcca tcagtatagc
    21121 tatggacaat gccggaaagg gttttgattg aacagggtaa aatcaccatc cctagcgtct
    21181 ggaaagaacc ggaagagatg ctggcggcaa tatcgcgcgc atcgtgcgtg acatcggcta
    21241 atgcctgcac ttcgcgcaga gaaaaatccg tttcgaggga taaggtctgg cgcgctgcct
    21301 ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg cagaacctgt aataagcgca
    21361 cgccataaat cgcgccgctg gcaccgctga tgcctacaat gagtcgtttc ataaatagtt
    21421 gccctgtcag acttgcaggc agactttgca ggatttcggc gggagttgca agtcagggtg
    21481 ccagaccggc accctcagcg aaggcatcat ccttcgttat gcatttcgag attttccact
    21541 tcgttctgac gttgcactgc tttggcgtca tcattacgta acgtatcgag gaaatcgagg
    21601 tagccctgat caacatcttt ggtgacgtag acgccgttga acaccgagca ttcaaactgc
    21661 tggatatccg gattttcagc gcgaacggcg tcgatcagat cgttcagatc ctggaaaatc
    21721 aacccgtcag caccgatgat ctggcgaatt tcatcaactt cgcgaccgtg agcgatcagt
    21781 tccgtggcgc tcggcatatc aataccataa acgttcggga agcgaatttc cggtgccgca
    21841 gaagcgaggt acactttctt cgctccggct tcgcgtgcca tctcgataat ctgctcagaa
    21901 gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt ttttatcgcg gaactcggcg
    21961 cggttggcat tcagtttacg gcgcacggac ttacgacgca gctgctggcc cggcatgata
    22021 aaggtgcggc caacatagcg gtttttaacg aagccctggc ggtacggttt gcccagaata
    22081 cgagcaattt ccagcgcgat atcacacgag gtttctggga tcgggatcac cacgtcgata
    22141 tccagatctt cccattcgcg ggcaattttc tcgcccagtt tcgtgcccat attcacacgc
    22201 gcgctgtaaa cggaaatttt gtcgatgaac gagtccgggc gagcaaagta tacatactca
    22261 aacaggcacg gattgctgac cggattgtca gcacattgac gggtaaacaa ctgcccttct
    22321 tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca ggaaatcaaa gcccagcgta
    22381 tcgagcgcta cgctttcgga agcgaccata tattctgtac ggttctcgtc aatatcacgt
    22441 tttcccagta ccagcggacg aatcccgttt ggatcgcgga aagcaaccat accgtggccg
    22501 ataatcatcg ccacacaggc atatgcgccg cggattaagc ggtttgtagc agcaatggca
    22561 gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt tgtccagctc gctggcgaag
    22621 atattaagca gaatttccga gtcggaagtg gtgttgatgt ggcggcgttt ttcttcaaac
    22681 agttttttac gcaactcgtg agcgttggtc agattgccgt tgtgggcaag cgtaatgcca
    22741 tacggggagt taacgtaaaa cggctgcgct tcagaggcgc tggagctgcc agccgtaggg
    22801 taacgcacat gaccaatgcc catattgccc tgcaaacgct gcatatggcg agcttcaaat
    22861 acatcgctca ccagcccgtt cgctttacgc aaacggaagc agttattggc atctatggtg
    22921 atgatgccgg cggcatcctg accgcgatgc tgaagcaccg ttaaggcatc ataaatcgac
    22981 tggttaaccg gcataacacc ggcgataccg acaataccgc acatacgtct ttttcctcgt
    23041 taagccacat ctcagagcac ttacgctctg ggcaagaaac ttgacgagct ttgcagataa
    23101 tcaaaaaacc atctgatgat aaaactgaac tgcgggatca gctgtgattt gctccagtct
    23161 tcgcttttcg acaccccggt aaaggagtcg agaaagaata gaatggcggc aacaatcaac
    23221 acaccgcgca acgcaccgaa acagacgccc agcacccgat cggtgcctga caaccccgtt
    23281 ttctccacca gctggcctat cacgaagttc acgatagcac caacgatcag ggtagcgata
    23341 aatagtaccg cgatggcaat cccatttcga accagttcgt cttcaaagcc cgtaaaccag
    23401 actgacaggt aagtgtagta atgactggca acaaagaaag cacaacccca tgtcaccagc
    23461 gataacgctt cacgaacaaa gccgcggatc aggctaacca gagaggaaaa agcaatcacc
    23521 gcgattatgg cgtaatcaat ccagaccata tgtgtcccac gattttacgc cctgtcatcc
    23581 tgttcggggc gcattctaac agaaaaagaa aacgtttgcg tagggatttc cttcccgcgc
    23641 atcaataaaa atggcgctga aaaaatattc aacgccatcg actttttatg cctttgcggc
    23701 atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc cgacctacgg ttctaacact
    23761 gcccaggcct gataagacgc gccagcgtcg catcaggcaa gactgtatta attcggcgta
    23821 tagcccatta ccacgccact taagccagaa agttgcttca actcacccag cgaacctttc
    23881 agcttatctt tcgaggcatc cggcccaacc agaatacggg taattttacc ctgcactggc
    23941 gtggatggcg acgtataaac ccgataaccg gcaccgcgca gcttaccgac aatctcattc
    24001 actttatcgg catttttcag cgcacccagt tgcacaacat aggctttacc cgttggcgct
    24061 gctttttctt ccacgaccgg cttcggttct ggcttcggcg caggtggtgc ttcaaccttc
    24121 ggtttaggcg gctccaccgg tttcggcttc ggtggggcga ccggtgcagg ttccggttca
    24181 aactcggtgt tattggctgc aatagtggcc ggatcgagcg acggtgctgc ggcatcacct
    24241 gcccgcacct cttccgctgc gccttccggc ggctgcgtcg gcaacgcctg ggtggcggct
    24301 ggcatcatat caggctcatc acgatcgccc gctttcggca ccagcgggat agccgcgaac
    24361 tcatcctgat aatgtttttt ctgcccgtcc agcagccctg gaagtacaat cacccccagc
    24421 gccaccagca cgatcgtgcc cactaaccga ttctgaaact tacttgccac cgcttctcct
    24481 cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa gaaccacaca ccagcacggt
    24541 gtcttccgct ttagcgtccg ccattgcggc atcccatgcc tgcgcaacgc tatcaaatga
    24601 tttgccgtta cccaaatgct caagcagttg ttctgccgtg gcaccgcgcg gcccttccag
    24661 tggcgcacaa taccagtcat caaccacgct tttcaaccag gccagagttc cggcaatatc
    24721 tttatcatgt agcataccga taaccgccag cacgcgcccg tttttcggta gcgctttcat
    24781 acgcccggtg agatattccg ccgcatgtgg attatgcgcg acatcaaaaa taacgcgtgg
    24841 cgactcgctc acaatctgga aacgtcccgg caaaattgcg ctggcaatcc cgtcgcgaat
    24901 ggcattttca ctgacttcca gcccgctggc acgcagtgcc gccagcgctg ttgcggcatt
    24961 cggttgcggg acaagcggca acggcagatt ttccagcgtg ccgtgagcat cgctaaacgc
    25021 ccagtcatga tcggtgacgg aatagttcca ctcaacgccc cgacgttgta acagtgcacc
    25081 tttttcctgc gccacatcag caatggtaga aggcatttcc ggctcaccga caattgccgg
    25141 ttttgcgctg cgaaagatgc ctgctttctc gcgaccaata ctttcgcgat ctggtcccag
    25201 ccagtcggta tgatccagcg caatgctggt tactacagcc acatcggcat cgacaatatt
    25261 ggttgcgtcc agacgaccgc ccagccctac ttccagaatc actacgtcaa gttgtgcctg
    25321 tttgaacagc cacaacgccg acagcgtacc gtactcgaaa taggtcaggg aaatatcacc
    25381 gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc gattccggca attcctgccc
    25441 ctgcacacgt acgcgctcgg tataacgcac cagatgcggt gaactgtaga cgcccacttt
    25501 gtaccctgcc gccatcagaa tcgactccag cgtacggcag gtggtgcctt tgccattcgt
    25561 acccgcaacg gtaaacacaa atggcgctgg tttcaggacg ccaagacgcg ccgcgacctg
    25621 gctcacgcgc tcaaggccga gatcgatagt tttactgtgc aggttttcca gataagaaag
    25681 ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg ataatcatgg tatccgctga
    25741 ttcgttacgg tgagaatagc aaaagggcag agccagtggc cctgccctta tcagttatca
    25801 ggcctcaggt tcctgatccg gtaccggggg taccactacg ccttcacgcg gcgcttcagg
    25861 attcggcgct ggcagattca tcaacttcgc cagaatgctc gccagtttca ggcgcatttc
    25921 cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc aggaattcac tgcgctggaa
    25981 tcctggcggc agtttttcgc gaacggtctg ttcgataaca cgcggaccgg caaagccgat
    26041 taacgctttc ggttcagcga tgttgagatc gcccagcatg gcgaaacttg cagaaacacc
    26101 gcccatcgtc gggtcggtca gcacggagat gtacggcaag ccgcgctcct gcatttttgc
    26161 cagtgccgca gaggttttcg ccatctgcat cagcgacatc agtgcttcct gcatacgtgc
    26221 gccaccagag gcggagaagc agatcagcgg gcagttatct tccagcgcct gctcaacggc
    26281 acgcacgaaa cgtgcaccca caacagaccc cattgaaccg cccataaagg cgaactcgaa
    26341 tgccgcagcg acaaccggca ttccatacag agtgcctttc atcaccacca gcgcatcttt
    26401 ttcgccagtt tctttctgcg cagatgccag acggtcttta tacttcttgg agtcacgaaa
    26461 cttcagcaca tctttcggct caagctcgct acccagctcc acaaggcttc cttcatctaa
    26521 caggctatgc aggcgattac gcgctgtcat acgcatgtga tggtcacact tcggacagac
    26581 ctcaagatta cgttccagct cagcgcggta taaaacctga ccgcagctat cacacttagt
    26641 ccacacccct tcaggaatgc tcgccttgcg ggtgggagta atgttgcttt taattcgttc
    26701 aatccagctc attagggacc tttctgtctg aacctggttc gatgccagtt ttatctttgg
    26761 ggacgcataa tgccattttt gcccccaaca gaccatgaat gttgcacatt aaaacataac
    26821 agcccgaaac tttggataaa aaagtggtcg aaccgcggag ttacttttta ttttgcggcg
    26881 cgtgccgcag cgcgtttgtg acgaataaat tcgaaaaccc caggcaatat tgaaacaaaa
    26941 ataatgccaa caatcatcag cttaaggttg ctctgaatga aggggagcgt gccgaagaaa
    27001 taacccgcgt aggtaaaaat cagtacccac aacagtgcgc cgatcacgtt ataagcggcg
    27061 aaatgacggt acgacatgtg gcccattccc gcaacaaacg gcgcgaacgt tctgacgatc
    27121 ggtacaaaac gggcgagaat aatcgttttg ccgccatgtc tctcgtaaaa ctgatgagtc
    27181 ttatcaagat aactgcgacg aaagattttc gaatccgggt tactgaacag tttgtcacca
    27241 aataaccgtc caatggtata gttcactgca tcaccgacaa tagcggcaat caacataagc
    27301 accaccatca tatggacatt gagatcgtta gtttccagcg acgccaatgc accagcaaca
    27361 aacagcagcg aatcaccagg caggaatggc gttaccacca gccccgtttc gcagaacaga
    27421 atcaaaaaca gaatggcata aacccagacg ccgtactccg cgaccagctc tgccagatgc
    27481 acgtcaatgt gcaggataaa atcgatgagg aaataaatca ggtccatatt tgcctatgcc
    27541 ttgtactcgt catctttcag gctgtaacta cgttggctac agcctgaaat gttccgagca
    27601 ttattctcgt tagtccgcca gaaatagcgg gcccattggc ggttttggaa gatcataccg
    27661 gtcagggtaa tccaccgcga ccagatacag cccttccgct tttgccgttg ctgccgccag
    27721 cgttctgtcc tttgccgcca gcaactctgc tatccagctc tccggctggt tgtgggcacc
    27781 gacttccatc aggctgccga caatattcct gaccatatga tgtacaaagg cattcgcttt
    27841 gatatctacc accacataag gaccgtgacg cgtgacatta atatgcataa cgttgcgcca
    27901 cggggttcgg gactggcact gcaccgcacg gaacgaggtg aaatcattct cgcccagcaa
    27961 gcattgcgca gcccgatgca tccgttcagc gtccagcggt tcgtaaaaat gggttacccc
    28021 tttactcagt accgccgggc gcagccgatg attgtagatg atgtagcgat aacggcgagc
    28081 cgtggcgcta aatcgggcat gaaaatcatc aggtacagct ttaacccaac gcacggcgat
    28141 gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc gcgtctttgc gctgcgcggt
    28201 cgtttcgaaa tgcactacct gcccggtacc gtgtacacca gcgtcagtac gcccggcgca
    28261 gaagacggta atgggttcgt tcgccacctg ggagagcgcc ttttccagct tctcctgcac
    28321 actgcggact tcattctgcc gttgccagcc gtaatactta ctgccgtcgt actcaatgcc
    28381 cagcgcaatt ttataaactg gcggttgttg ctggtcggac attagtacag atactcctgc
    28441 accagtttct cggcgatttt tactgccatc agcgcgccgc caaagcgaac gttatcggcc
    28501 accgaccaga actggacttg ctccggcata ccgtagtcat tacgcacgca gccaacagaa
    28561 agatgcggcg tacccgaagc atcccctacc tgagtcggga attcgttctc ttcagagagc
    28621 acaatatctt cgccctgagc aaacacatca cacgcttctt ctgccgccag cggacgcagt
    28681 gcttcaaagt tgaccatctg ggcatgaccg tagaataccg gtgcctggac gacgcttgcc
    28741 gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt cgtcaacgat acgacgttct
    28801 tcacgcacgc taccttcgct atccggcaac aacggcagca tattgaacgc cagttgacga
    28861 ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca atttcgcact ctgccccgct
    28921 aacgcatcga ccgctttttt gccctgggcg gaggctgaaa tcaggctggt aacactgata
    28981 cacgacaaac cgccctgatc gattaacggt ttcagtgccg ccagcagctg gctggtcaga
    29041 ctgtctggta cggcgatgac attccggttg cggtaatcgg tcagtacaaa cgggtttact
    29101 tccggcacca ccagcggtac gtcgggttcg agagcaaaca gtccgctgct gtcgatcacc
    29161 aggcaacctg agttggtcgc ttcttcaacc caggcagcgg tagcttcttt gcctgcgaca
    29221 aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg catcctgcac ggtgattgtc
    29281 ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac gtgccagtgc ataaatttcc
    29341 ccaaccggga actgacgttc agccagcgtt tcaagcaggg cttcgcccac agcgccagtt
    29401 gcgcccagga cggcaatgtt ccagccttca gacatggtgg tttactccag aaatagcaaa
    29461 gctccctgcc aatgttacag cagagagcat gaagaagaga ttaacgtgcc ggatgatgaa
    29521 cggcgttaaa acccagttta cacagcaatg atgccgcact ggcgtcatca caaattacat
    29581 acagagatga ccattcacgg cgctcaagat agtttttgcg cagtttatcg aactcacccg
    29641 gtatcccggc gactttacgc agcggtgcgt catcgcggcg cacatcatac accaaatgca
    29701 ccagcctttt cagcgtcggt tgatcgagcg ggccatgcag cgtaatgcga ccaaactctg
    29761 gcgcgggcag taatgtatcc agcgcaacgt gctgttcatg cccaataaac ttgctatagg
    29821 cttcaaacac ttgcgtagta ccgcgtgctt taccttccag ggtataacct gcgatatgcg
    29881 gcgtgccgat atccactttt gtcagcaact ccacgttaag ttccggttcg ccttcccaga
    29941 catccagcac tacgcttaac ttctggcctt cattcaggca ggtcagcaac gcagtattat
    30001 cgacgactgc gccacggcag gcgttaatca gaatcgctcc cggcttcagg ctacggatca
    30061 gtttttcatc cgccagatgc agcgttttgt acggcccatc tttgaagagt ggcgtatgga
    30121 aagtcagaat atccgcatgc tgaactaatt catccagcga gcggaaatta ccctcatccc
    30181 cacggtcagc gcgaggcgga tcgcaaagca aggttttaat ccctaacgct tccagtcgcg
    30241 cttgcaatcg acgtccaacg ttaccaacgc ccacgatccc caccgtacgg tcgtgcagtg
    30301 aaaatccatc gcgttcggca agcatcagca gggaggagaa aacatattcc accaccgcaa
    30361 tcgcattaca gccaggtgcc gcggaaaaac caattcccgc ctgctttaac catgcttcat
    30421 cgacatggtc ggtccccgct gtggcagtac caacaaattt aatgggtttt cctgccagca
    30481 aagattcatt cactttcgtg accgaacgca ccatcagcgc atccgcgtct gccagttgag
    30541 cgacggggat tggacgcccg ggaaccgcgg tcacctcacc caaacggcta aataagtcgc
    30601 gggcataagg catattttca tcaacaagga ttttcacgtt tgtgttacct gtatgagacg
    30661 agagttaacc ggacaagtgt gccataatct cgcggccagg catacttgcg aagatttcag
    30721 gtataaggat acgtaatgat acaacctatt tccggccctc ctcctgggca gccaccaagt
    30781 cagggagata acctgccgtc tggcgcgggc aatcagcctt tatccagtca gcaacgtact
    30841 tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc aacagcaaag agcagaactg
    30901 tgggcgggta tcaggcacga tattggtctg tcgggagatt caccgctgct ttcgcgtcac
    30961 ttccctgccg ctgaacacaa cctggcgcaa cgtctgctgg ccgcgcaaaa aagccattct
    31021 gcccgccagc ttttagcgca attaggggag tatttacgtc tggggaataa tcgtcaggcg
    31081 gtcacggatt atatccgtca taactttggt cagacgccgc tgaatcagct ctcaccggag
    31141 caattaaaaa ccattctcac cctgttgcag gaagggaaga tggttattcc gcaaccgcag
    31201 cagcgcgagg cgaccgaccg tcctttatta ccggcggagc acaatgcgct caaacagctg
    31261 gtgaccaaac ttgcggcggc aacgggggaa cccagcaaac agatctggca atcgatgctg
    31321 gaactttccg gggtgaaaga tggcgagtta attccagcga aactgtttaa ccatctggtg
    31381 acctggctgc aggcgcgcca gacgttaagc cagcaaaata cgccgacgct ggaatcacta
    31441 cagatggcgc taaaacaacc tttagatgcc agtgaattgg cggcgttatc ggcatatatc
    31501 cagcaaaaat atggcctttc tgcgcaatca tcgctttctt ctgcccaggc cgaggatatt
    31561 cttaatcagc tttatcaacg gcgggttaaa gggattgatc cgcgtgatat gcaaccgctg
    31621 cttaatcctt ttccaccgat gatggacacg ttgcaaaata tggcaacgcg tcccgcgctg
    31681 tggatactgt tagtcgcgat tatcctgatg ctggtctggc tggttcgtta gccccgacga
    31741 aatgacagta tcgtgacaat aattcccagc accgcagaga tcgccccggc aagaaatacc
    31801 gaagagtaac caaacgtggt cgccagcatt cccgccagcg gcccggagac gccgagggcg
    31861 atatcctgaa acgcggcgta accgcccagt gcggtgccgc gaacttgtga ggggacgcgt
    31921 ttaaccacct ccacgcccag cgcaggaaag ataagcgaac atccggctcc ggttaacgcc
    31981 gcgcccgcta atgcgaccca cgcccctggg gcttgccaga gcagcaacaa gcccaccgtt
    32041 tctacaagca gagagacaat cgccactttc acgccgccaa aacggtccgg catccagcca
    32101 aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg taagagtaaa gcccgccatc
    32161 gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc cgataaccgc aaaaccaacg
    32221 ccttgtagtg ccagccctaa ccctggtttc cagataagcc cgacaacgct ccacagcgat
    32281 ggacgttctc ccgccaaggc cggtactttg cgcactgtgc cgttacaggc ccatgccagt
    32341 aagggtaata ccattgtggt gatcgccagt gcggcaaaac cgtaatggct atgaatcaac
    32401 aggccaagcg gagcaccaac agcgagggca ccgtaaatcg ccattccatt ccatgacatc
    32461 actttgccag agtgttttgg ccctacgatg cctaaccccc aggtcagagc gcctgtcagt
    32521 aactggcttt caccaaagcc aagaatcaaa cgcccgacga ccaacagggc aaatttgaac
    32581 ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc cgccagccag accacaagct
    32641 aacatcccct gaagcgccga acgttttgca ccatattgat cggccagtcg cccggcatag
    32701 ccacgcgtca gcaccgtagc cagaaactga atcccgacgg caatcccgac catggtattg
    32761 ccatagccca gatcatgatg aacaaacagc gggataaccg gcaacggcag ccctacggtc
    32821 atgtaggtga gaaaaaccgc aaaagcgatg cggaagagcg aaaaattggc agaagatcgt
    32881 gtttcggttt ggcttacagc agtcatgcat tactccagaa tgcagcgcaa ggcgaggagt
    32941 atccccgtct catctctctg gtttcagggt tacggtgcgt tggcaggatt taacgcgtac
    33001 gtcttttcag aaggaaatcg acaaagcggg aagtttgcct ggaacaggcg gcgattgtca
    33061 atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt agtgccggag gccgcattag
    33121 cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac ctactccgac tactccgacc
    33181 tactgcgaat taatctttca gcttgcgcat taccagcgtg gcgttggtgc cgccgaagcc
    33241 gaagctgtta gacataacgg tagtcagttc gcgatcggtc gtttcggtca cgatgttcag
    33301 acccgcagcc tgctcgtcca gctcttcaat gttgatgctc ggggcgataa agccgtgttc
    33361 cagcatcagc agagagtaga tagcttcctg tacgccagca gcgcccagag agtgaccggt
    33421 catggctttg gttgcagaaa tcgccgggct cttatcgccg aacacttcac ggatagctgc
    33481 cagttctttc acgtcgccaa ccggagtcga agtaccgtga gagttcaggt aatcgattgg
    33541 ggtatcaacg ccatgcatcg ccatctgcat acaacgtact gcgccttcgc cagacggagc
    33601 aaccatgtct gcaccatcag aggttgcgcc gtagccaacg atttcagcat agatgtgagc
    33661 accacgcgcc agcgcgtgtt ccagctcttc aaccactacc ataccgccgc cgccagcgat
    33721 aacgaaaccg tcacggtgag cgtcgtaagt acgggaggct ttttccgggg tgtcgttgta
    33781 tttagtagac agcgcaccca tcgcgtcgaa ttcgcaggcc atttcccagc acagctcttc
    33841 gccgccgcca gcaaacacga tgtcctgttt gcccagttgg atctgctcta ctgcgttacc
    33901 gatacagtgt gcggaagtcg cacacgcgga gctgatggag tagttaacgc catgaatttt
    33961 aaacggcgtg gcgaggcagg cagaaacgcc ggatgccatc gctttggtga ccacatacgg
    34021 gccaaccgct ttcaggccgc gcgggccgcg cattgcgtca gcgccgaata cctggaaacg
    34081 cggggaaccg ccgccagaac ctgcaatcag gccaacgcgc gggttattct ggtaagcttc
    34141 cggagaaagg cccgcatctg cgattgcctg ctccatagaa aggaatgcat aaatggatgc
    34201 gtcgctcata aagcgcacaa ctttgcggtc aatgaggcca gtggtatcca gttttacgtt
    34261 gccccagacg tggctacgca tgccggaatc cttcagctcc tgagagaaag tgatccctga
    34321 acgtccttca cgcagagatg ccaggacttc ctgctggtta ttaccgatgc tggaaacaat
    34381 gcccaggcca gtaatcactg cacgtttcat tcaatacctc tgtaagtcgc acatagagta
    34441 agtttcgaat gcacaatagc gtacacttgt acgccgaaca agtccgatca gccatttaat
    34501 agagaaattt gcgcagcctt acacacatcg ctaagatcga gccaccgcct gtaagacgag
    34561 taacttacgt gaaacactac tccatacaac ctgccaacct cgaatttaat gctgagggta
    34621 cacctgtttc ccgagatttt gacgatgtct atttttccaa cgataacggg ctggaagaga
    34681 cgcgttatgt ttttctggga ggcaaccaat tagaggtacg ctttcctgag catccacatc
    34741 ctctgtttgt ggtagcagag agcggcttcg gcaccggatt aaacttcctg acgctatggc
    34801 aggcatttga tcagtttcgc gaagcgcatc cgcaagcgca attacaacgc ttacatttca
    34861 ttagttttga gaaatttccc ctcacccgtg cggatttagc cttagcgcat caacactggc
    34921 cggaactggc tccgtgggca gaacaacttc aggcgcagtg gccaatgccc ttgcccggtt
    34981 gccatcgttt attgctcgat gaaggccgcg tgacgctgga tttatggttt ggcgatatta
    35041 acgaactgac cagccaactg gacgattcgc taaatcaaaa agtagatgcc tggtttctgg
    35101 acggctttgc gccagcgaaa aacccggata tgtggacgca aaatctgttt aacgccatgg
    35161 caaggttggc gcgtccgggc ggcacgctag cgacatttac gtctgccggt tttgtccgcc
    35221 gcggtttgca ggatgccgga ttcacgatgc aaaaacgtaa gggctttggg cgcaaacggg
    35281 aaatgctttg cggggtgatg gaacagacat taccgctccc ctgctccgcg ccgtggttta
    35341 accgcacggg cagcagcaaa cgggaagcgg cgattatcgg cggtggtatt gccagcgcgt
    35401 tgttgtcgct ggcgctatta cggcgcggct ggcaggtaac gctttattgc gcggatgagg
    35461 cccccgcact gggtgcttcc ggcaatcgcc agggggcgct gtatccgtta ttaagcaaac
    35521 acgatgaggc gctaaaccgc tttttctcta atgcgtttac ttttgctcgt cggttttacg
    35581 accaattacc cgttaaattt gatcatgact ggtgcggcgt cacgcagtta ggctgggatg
    35641 agaaaagcca gcataaaatc gcacagatgt tgtcaatgga tttacccgca gaactggctg
    35701 tagccgttga ggcaaatgcg gttgaacaaa ttacgggcgt tgcgacaaat tgcagcggca
    35761 ttacttatcc gcaaggtggt tggctgtgcc cagcagaact gacccgtaat gtgctggaac
    35821 tggcgcaaca gcagggtttg cagatttatt atcaatatca gttacagaat ttatcccgta
    35881 aggatgactg ttggttgttg aattttgcag gagatcagca agcaacacac agcgtagtgg
    35941 tactggcgaa cgggcatcaa atcagccgat tcagccaaac gtcgactatc ccggtgtatt
    36001 cggttgccgg gcaggtcagc catattccga caacgccgga attggcagag ctgaagcagg
    36061 tgctgtgcta tgacggttat ctcacgccac aaaatccggc gaatcaacat cattgtattg
    36121 gtgccagtta tcatcgcggc agcgaagata cggcgtacag tgaggacgat cagcagcaga
    36181 atcgccagcg gttgattgat tgtttcccgc aggcacagtg ggcaaaagag gttgatgtca
    36241 gtgataaaga ggcgcgctgc ggtgtgcgtt gtgccacccg cgatcatctg ccaatggtag
    36301 gcaatgttcc cgattatgag gcaacactcg tggaatatgc gtcgttggcg gagcagaaag
    36361 atgaggcggt aagcgcgccg gtttttgacg atctctttat gtttgcggct ttaggttctc
    36421 gcggtttgtg ttctgccccg ctgtgtgccg agattctggc ggcgcagatg agcgacgaac
    36481 cgattccgat ggatgccagt acgctggcgg cgttaaaccc gaatcggtta tgggtgcgga
    36541 aattgttgaa gggtaaagcg gttaaggcgg ggtaatctgc tctcgcagtg tttaccggat
    36601 gcggcgtgaa cgccttatcc gacctacgtg tgacgtgtag gcctgataag acgcgataag
    36661 cgtcgcatca ggcatggtgc tccagacgcc gcaacttact gctgtgacgc ctgctgaaac
    36721 aggttttccc acatatcggt caccagcgcc tggtcacgcg gcgacaattc accggcacca
    36781 atggcttttt ccagactctg gctaacggtc gtatgcaccg cctgagcgga gtggtcatca
    36841 ccactttcca gttctgcgat ggctaatgtc aggtggccac gcaaataccc actggcaaac
    36901 aactcatcat cactggcatg gtcaaccata ccgtcgatta atgccagaat gcgtgattca
    36961 aactccgcga tcatcttctt tcctcattgt aaaacgtggc gctggcttta gttgagcgct
    37021 tccggccacg ggaactgttc cgccgtaagt tccggcgtgt gataataatt ttgtagtgct
    37081 ttaatgaagc gtgccgggcg ttcaggaatg ccgtttgcta ggtaatccat cacctgcgca
    37141 tgtacccgcc gttgaaacac cacacgatcc ggttcgaagt cgccttccag attgtcgcaa
    37201 ctgacgttaa acggatatcc cgccgccaca cagaacaacc agtcgagtgc ctgcggcttc
    37261 acttcaacat cttcaaactg gctttgcgtt tgggcatcac gcccatccgg gcaataccag
    37321 tagccgaaat caaccagctc acggcgcgct tttccggcaa tacaccagtg cgaaatctca
    37381 tgaattgcgc tggcataaaa gccgtgggca aagacaattc ggttatacgg tacttccgca
    37441 tcagcaggaa gatagatcgg ttcgtcgtcg cctttaatca gacgggtatt aaattcatcg
    37501 gcaaagcagc tattaaaaat ttcaatcaac tgctcatagt ggtgtgtact gttcattagt
    37561 tcatccccaa ccagtggagg atctcctgtc cgtggctatc gtaaagtaat ttggcactca
    37621 tcaccgccga gacgataaca atcatcgggc ggatcagctt ttgtcctttg ctcaacacca
    37681 gccgtgaacc catgcgcgcg ccgaggaact gccccaccag catgacaaag cccgtcgccc
    37741 aaatcacttt gccgccgaga ataaacagca gcagaccgcc aatgtttgac gttgcgttga
    37801 gtaatttggc gtgagccgtg gctttggcga ggttgaatcc gcacagcgta acgaaggcca
    37861 gcgtgtaaaa cgatccggca gccgggccaa agaatccgtc ataaaaaccg acgcagccac
    37921 cggcaattaa cgcgaagggt aaaccgtaca tccggcgctg gcgatcttct tcacccagct
    37981 ttggcatcag caagaaatag aggccgatac aaatcaccag aatgggcaaa atctgccgta
    38041 agacatcagc ctgaacgtac tgtaccaaca gtgcgccgct cattgagccg acaaaggtca
    38101 tggcaatatt gagtttctga tcgcttaaac taaccacttt gcggcgaata aagtagatag
    38161 tggcggaaat agagccgccg caggcttgca gtttattggt tgccagcgca ttagcgggag
    38221 acatccccgc tgccatcaat gccggaatgg tgagtaaccc accgccaccg gcaatcgagt
    38281 cgataaatcc cgccagcatg gcgacaaaaa agagaactcc cagcaacagc ggggaaacca
    38341 taaacaggct attaaacgtt tccattagat cacgtgctca tccagtagcg cctggcagga
    38401 aggcggcaac ggaggcggtg tcttcttctc aggctttgtt gttcccggtt ttggaggtgc
    38461 aaaccagctt tgcagttctg ccccgcaacc atcgcctggt ggcggtaaag gttgatcttc
    38521 acactccaga ctatcggcag gacaacgtaa tcgtacatgc atatgcgcgc gatgctggaa
    38581 ccagggtcgc actttgcgca accagtcgcg atcggtgccc gcatcaaggc aaagttgttg
    38641 tttaatcgcc ggattaacaa aaatgcgtgt gacgtctttg tcctgggcgg cgagtttgat
    38701 caagctgaaa atttctggct tccacagcgt ggagacaacg tgtttaccgt cgcgggaaac
    38761 taagtccagt gcttgcgggc gcaagagctg cgcggaggtc cagcgagttt tcggcagttg
    38821 cagaaagata tcgacatcca gtccggtctg gtggctggca tgaccgccgt tgaaacgccc
    38881 accagcgggc atccccatat cgccaatcag caccgtaccc atgcccagat tgctcacctg
    38941 gctactcaga cgctggataa acatcaccag atccgggtga ccgaaatagc gacgctgatc
    39001 ggtacgcatg acctgataat gttcggactg tatcggcagc gtgtcagcac cgacgataca
    39061 gccattagaa aaactgccta tcgattgtgc gctacccggc acaggttggg ttattttttg
    39121 ccacggcgtc gctgccaggc tagcgctact ggcaagcaga gccagcagcg caatcgcggt
    39181 tttgttcatt ttttaccagc gtggaatatc agtcttcaca tcggcatttt gcgcccgctg
    39241 ccgtaacagg tgatccatta aaacgatcgc cagcatcgct tcagcgatcg gcactgcacg
    39301 gatcccgaca cagggatcgt gacggccttt ggtgatcatc tcaacttctt cgccaaagcg
    39361 gttaatagtg cgccccggca cggtaatgct ggaggtcggt ttcagcgcca tatgagcaat
    39421 gatttgctgc ccgctgctga taccgccgag aatgccgccc gcatggttgc tctggaaacc
    39481 gtctttggtg atttcgtcgc ggttctggct gccgcgcagc gccactacgt caaaaccgtc
    39541 gccaatttcc acgcctttca ccgcgttgat gctcatcagc gcatgggcga tgtcggcatc
    39601 caggcggtca aagaccggct cgccaagtcc ggcggggaca ccactggcaa caacggtgac
    39661 tttcgcgccg atggagtcgc cctctttttt cagcgcgcgc atcagttcat ctaacgcgtc
    39721 gattttgtcg gggtccgggc agaagaacgg attttgttca accagcgacc agtctttgat
    39781 ttccagcgga atgtcgccca tctgggtcaa gcagccgcgg atttcaatac caaatttctc
    39841 ggcgagatat tttttggcaa tcgcccctgc cgccacgcgc atggcggttt cgcgggcgga
    39901 agaacgtcca ccgccgcgat aatcgcgcag accgtatttt tgttcgtagg tgtaatcagc
    39961 atggcctggg cggaaaacgt ctttaattgc gctgtaatcc tgagagcgct ggtcagtgtt
    40021 ttcgatcaac aagccaatgc tggtgccagt agtaacgcct tcaaaaacac cggagagaat
    40081 tttgacctga tccggctcgc ggcgctgggt ggtatagcgc gatgtcccag ggcgacgacg
    40141 gtcgaggtca tgttgcaggt ccgcttccgt cagcggaatg cctggcggaa caccatcgac
    40201 gatgcagccg agcgccagcc catgcgattc gccgaaggtg gttacgcgaa agagttgtcc
    40261 aattgtgttt ccagccatca cggctccgtt attgttgtgt ttgcgtgttt acttaatctt
    40321 tataaatcgc gaaatattca cgggccgcca gcagctgctc tttggtgagc ataaacacac
    40381 catcgccgcc gttatcaaac tccagccagg tgaacggaac atccggatat tgttccataa
    40441 gatgtaccat gctgttgccg acttcacaaa tcaacacgcc atcatcagca aggtaatctg
    40501 ccgcgtttcc aagaatgcga cgtgtcagtt taaggccgtc ggtgccagat gccaggccca
    40561 gttccggctc gtgacggtat tcgtttggca ggtcggacat atcttccgcg tcgacatacg
    40621 gcgggttagt gacaatcaga tcgtattgca ctttcggcaa atcacggaac agatcggaac
    40681 gaatcggaat gacgttgtgg atcagaccgt gttcttcgat gttctgttcg gcaaccgcca
    40741 gcgcgtcggg agagatatct accgcgtcga cttccgcttc cgggaaggca taagcacagg
    40801 caatggcgat gcagccgcta ccggtacaca tatctaaaat atgctgcggt tgcttgctga
    40861 tgagtccggc aaatttattg ttgatcagtt caccaatcgg cgagcgcggc accagcacgc
    40921 gttcatcgac gtaaaattca tgaccgcaga accaggcttt gttggtcagg taagccaccg
    40981 gaatgcgctc gttgacgcgg cggatcacgc gttcaacaat acggtgtttt tcgctggagg
    41041 tcagacgcgc ggtgcgcata tcttccggga tatccagcgg caggtagagc gatggtaaca
    41101 ccagctgtac ggcttcatcc cacgggttat cggtgccgtg accgtaccag atatttgccg
    41161 cgctgaagcg gcttaccgac cagcgcaaca tgtcctgaat ggtttgcagc tcatttactg
    41221 cttcatcaac gaaaatttta tccacgtatt cctccagggc atgatcgcaa taatttcggc
    41281 ggctagtttg ccatgaagat gacgataaat cagcattcac gcgcggtgag tgaggaaaaa
    41341 tacgtttaaa acgatcgatt gtgctacgag tcgggtaaac tgtaggaaaa ttagaaatag
    41401 agacagataa atgaaaaaga aaacaacact tagcgaggag gaccaggctc tgtttcgcca
    41461 gttgatggcg gggactcgca agattaagca ggacacgatt gtccaccgac cgcagcgtaa
    41521 aaaaatcagc gaagtgccgg tgaaacgctt gatccaggag caggctgatg ccagccatta
    41581 tttctccgat gagtttcagc cgttattaaa taccgaaggt ccggtgaaat atgttcgccc
    41641 ggatgtcagc cattttgagg cgaagaaact gcgccgcggc gattattcac cggaattgtt
    41701 tttggattta cacggtctga cgcagctgca ggccaagcag gaactgggcg cgttgattgc
    41761 cgcctgtcgc cgcgaacatg tgttttgtgc ctgcgtgatg catgggcacg ggaagcatat
    41821 tttgaagcaa caaacaccgc tgtggctggc acaacatccg catgtgatgg catttcacca
    41881 ggcaccgaaa gagtacggcg gtgatgcggc tttgttggtg ttgattgaag tggaagagtg
    41941 gttgccgccg gagttgccct gaatgctggc agcaaatgcc ggagcagcgt aaacgcctta
    42001 tccggcctac aaatatgtga taattcaata aattgcaatt cacttcgtag gcctgataag
    42061 acgcatcaga gcaatactac gaactacagt tcattcaata catactgaaa acgacacgtc
    42121 gaatcaatcg gttgcccact actggtattc acattcacca caatatcgtg tagtacttta
    42181 tccgttaccg gatgacgagt tggcgtaacc gttaccgttg atttatcaca accgtaagag
    42241 gtaaaggtta acaattctga taccgcgcgg cagtatgctc ctctgccatc attatcagaa
    42301 aaattactgc catcacagag tctgccgcct tccatcaagt aggtcgaaag cgtggtggtc
    42361 actgttgacc ccgtggactg aattcgtaac gccttattat ggctgacata attttcactc
    42421 caggagatat cgtatgaagc gaccggtatc gttgggcata ttgtgttatg cactgtaggg
    42481 gccgtatagc ttccaaatcc gggtaaatct gtataggaat aaaaggatgc gatccaaggg
    42541 caaccataat catcagatgc aacggtaatt ttaaacgtca tatatgcaca acgaaaaatt
    42601 ttcgtatcta tgccagggca atttgagtta ttaattgtat caaagtcagt cagtaccaaa
    42661 gttggagctg aacccgaccc agaccaggaa ataacccgca acgtatcgcc tttatacgca
    42721 aaagaatcat atttcccata tgcaacctcc tttggcgtta tcaccgtaac attgaaaaca
    42781 ctgctcgcta cagcaccggt taccttgtcc atggcataat aaactctcac actgtcaaaa
    42841 ctcgcactca taagtttatc tccagcataa ctcttagatg cgccgacaac actcagaacg
    42901 agcagcatta ggctcgcgaa aattatggtc cattttttca tttcagaaat actcaaaggt
    42961 tgccgtcact gtggcactaa agtctccgga cgttacatct cgcccgctct ttgcctgtaa
    43021 ccaggtatta aaatttaggc tgttatttcc ctgattcagc gggagcgtga cgcctgttgt
    43081 attgttgatt ggcaccgatg tgcctgccgc agtttcaata ccaatcccag cgccggatgc
    43141 tgatgacgag gagtcaaagg ccagaaagcc gggtagagca ctatcttctg ttcctgtcaa
    43201 cgttactttg acattaaaca atgtagaact atgacaatct ttcaattgaa acaccaatgg
    43261 cactcgctct gactgcccaa ggttcattaa atcgctggcg gcaatggcag gaaaatgtac
    43321 ttctgccaac tggctaccat taatcactgg cgtacacgat ttactgatta aattgccggt
    43381 aaagtgcaaa ttatctcctg ctattgtggt tccactgccc agcaacatca ggctactaat
    43441 aaaaaacaac gtacgctttt tcttcattgg taatctacct gtaacgtggc ccaggcctca
    43501 aagtccgcct ctggcagaac gacaccactc ttttttactg gcacagccgt taacaccggt
    43561 aaatcggttt cattcaccac aagtggcgta ttgatcgtaa aactctgccc tgcctgtttg
    43621 agctgaatgc ctaaaccagt gatattgctg cttactgcag cattatcaaa atcgcttggt
    43681 gtgccactcc agctcaaggt catttccatt aatgggtcgc gaaccgccgt atcgcaggta
    43741 atggaatacg gcacggtttg ctcataattg ctgccattaa tgtcatcaat aagcacatcc
    43801 tgaaaagtta tttcaatggt ttgagcgttg ctaatcgtgc agttaggtgg ctgcactaaa
    43861 taaccgtgaa aagagacgtt tgagagtgcc atcgagcaaa agccccacag caaaataagc
    43921 gagatccgtt tcattggtaa tccaccacaa ttgtggcact ggcgttaaat tcagctgccg
    43981 ctaacgttgt accactctgt ttcaccggca cagcttccag aacgggaact cccgaggaaa
    44041 aattaaatgg caaccatgag ccagaggtta agtcgagtat tgtgtgatca ctacttttct
    44101 gaacccgaat accaaaccca ggaatccccg tgctgataac tgtttcaccg ttaatcacga
    44161 ccgttgtcgc ctgcatctgt agacgcaggt catccattgc cagattgtta caaacaagcg
    44221 agtagtcaat gggacgttta taatcaaccc cattgatctt attaatgaat acattaccaa
    44281 actcaactgc cgcaccattt accgtacaag gaggaggggc atcgacaagc acccgtaaat
    44341 tcatacttac tgacttactc gctgcaactg caaagccgct accaacacaa catatcagta
    44401 acccagttac cgcccagaca gaacaagatc ctttcatact ggctctcccg gcctcagcct
    44461 ttgcggctct gctcttcatc gacctggcaa ctgttaccgt tgcaagtgaa aaagagcggc
    44521 atccgagcgc catagtcgtt gacataggtc agtaccggca cgctacccat cttcacattc
    44581 agcggtgcag aggttttcgg cgacatcact attggcgaga aaccggctgc cggattgcca
    44641 tttttctgtg tgctggcatt actgaaaatg acgtaatacg gtgtcgggtt attgacggta
    44701 aattcctgac cgttgcgggt taaagtaacc ttatattgcc aggggttttt catgctgacg
    44761 ttctccagcg ccttcggtcg ccagaacaac ttaatacgcg tttgtagtgc tatttgcagc
    44821 gtgttggctt tgtttgattt tggcgggatc tcacgcacgt tgaagtagaa caggctttca
    44881 cgatcggcag gcaatttgtt gatatccggc attccctgca cttttacctg cccgttcatc
    44941 attgaatcga tgcgttgcac cggtggcaga acagttaacg gtgaactgat cttgttaccc
    45001 ttttcatctt cgatccagct ttgggccaga taaggcagtt tcggatcgtt attgcgcaac
    45061 gtcacgctga ttgatttgtc gctttcatta aatatcaaac gggtacgatc tggcgttaca
    45121 gaagcctggg cagacaaact ggcagcgcta agcagtaacg ctagcgtcgt agcacccaat
    45181 ttggcagagc aaagtaaatc agacatggat cattccttaa tcacgttatt ggttagctga
    45241 aacagaaaca gggggattgg tgggtgtcac ctgttgcgtc tgctcctgaa tgacaggctt
    45301 tatgtcatca ggaacaaccg gcgcgacgtt gcctttatgc tggcatggca gtaacaagcc
    45361 gttaaacaga tcggcaggca gcgggtccgg caggttgata tcgcaatgcg caacaccact
    45421 ccagaacaca ctcatgtgtt cgccaggttt caccccagcc agataaacat tgccgtcatc
    45481 atcgacaagg cccactgtct gctcgttatc attttttact tctgcgccaa acggaggatg
    45541 gctgccgtct tgtaaacgca gcaccgccat cgctttttga ccactgatga cggcaaattt
    45601 gcggtagcca atcgcccctt cagttagcgt ggcttgtacc accgactggg ttgcttcggc
    45661 gttttccggc agtttgttga ggtcgatata cgcctgattg cggtaatagt tattgacgtc
    45721 agaaacgacg gctttaccaa acatattggt ataaacagca gcaccgttac cttctaccgg
    45781 aacatcggca acgccatcgg catcaatcaa caagcgtgtc ccgcccatat tctgggtacg
    45841 gtgaagtgcg ccaccgtggg cagtaagcgt tgcgccgccc tgtaacgaca agcccgcaga
    45901 ggtgtattgc ccttcatggt agttcgcgct gaggtcaacc tgcgccagcg aaccatcatg
    45961 gctgtaatag ccgtcaacgc tggtgtgttt gtcactggtg ccgacgttca actgatagtg
    46021 agtcgcgtca tcgacacggc tgaaataacc gacctgactg ctgtccgtcc cactgccata
    46081 gttgccgtta tagctaacgg tactgttgtc gccccacggc atactgagcg aaatgtacat
    46141 gcccttatcc gcccggttgt catactcgta gcggtagcca gtcagggaaa cgctcatatt
    46201 acgaatgctg cccatattga aatagtggga gagcatgatg ttgtagtttg tctgctcctc
    46261 acgatcccag taagtatggc gggtatagtt gagataaacc gaaacacccg catcgcggaa
    46321 gttctggtta taagtggcgg tgtacatctc tttgtcgttg cccgtgcgta ccattccgct
    46381 gtcactggca tccagatact cgctcatggt cataaagttc tcttccgaga agcgatatcc
    46441 agcaaaagta acgcggctgt tgagctggtc aaagtctttg gaataactca cacggaagga
    46501 gttaccgtcc agcgaacctt tgccataagc ggtgtcttta tccagtttgg tgtgcgagtg
    46561 agtaacatca aacgcgaccg cgccgaacgt agacaaatcg cgaccaacgc caagtgcggc
    46621 agattggtag ttttcatccc ccagcgcgcc accatagagc gaccaaccat tagcgatccc
    46681 ccaggaagct tcagcaccag agaaaaattc gccttcaaca tgatgccccc actcttgcgg
    46741 acgacccatc atgatcttat agcgcacctg acctggacga gtgaggtatg gcatcgaggc
    46801 ggtgctgatg tcatattcct gcacctggcc gttctgttct tcaatgcgaa tatgcaacgt
    46861 accggagaca gaatcaccaa gatcctgaat acgaaacggt ccagccggaa cctgcgtttc
    46921 gtaaatcaca cgccccatct ggctgacggt cacttttgct gtggtgtgtg ccacgccgga
    46981 aatgtctggc gcgtagccgc gcaggttggg aggcaacatt tgatcgtcag tgctgacact
    47041 gccaccaaca taattaaaac cgtcgaaaat atcggaattg aggtaatctt cgcccagcgc
    47101 cagtttggct tttaatgacg gtaacgcccg ccaggcataa tagcgactcc actcccattt
    47161 tttttgtgtg tcatcgccgc taaattcctc gtcatcatca ttactgcggg tatgttgata
    47221 gttggtctgc cagtcagcgc gcatacgcca tggccccagg ttaaccccga ccgtcccgtt
    47281 gccgctgatc tcgttactat catcaccgcc attttcttcg tgccgggttt gtgcgttgat
    47341 gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg gatcccagtc
    47401 gggataagta tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg actggcttaa
    47461 atcagcctta atttccatgc cttccagttg accggatttc aggcatttag catcgtggct
    47521 ccattgcaga tttttcgcca cgtcttcttt taaaccaaac tgcgctacca gttccggtgt
    47581 cagacaagca tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa tatcgtactc
    47641 ttccgccaat ggctgtttat ttagttgaac ctgtaaattg tattttccgg gctcaacata
    47701 tccctggctg gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt ccagaaaacg
    47761 ggaatcaaac tgaatgtcgt cttcagccca gacactgcta taactccctg acattgccag
    47821 cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca tacacgtttc
    47881 tatccgtttt tattcttgtt cggtcaaaat ccatttaaat cccccatagt agtgtcgcta
    47941 tgggggtatg ccttacaggt aagtaatctg gaaggtggta tttgcttcga aattacccag
    48001 atctggcgca tcagctgcgc cgaccagcca ggctttaaag ttcagtgtct gcttcgcttt
    48061 gcctttattg gtagaagtgt ctttcactat tttctggtca atacccatgc cgcttttgta
    48121 agaggtaccc agagagtcac cgatcgccag gctaacattg ttaaatgccg caccggtatc
    48181 ggtattgaaa atggtgtaat aattgccgct atttgcagaa gaaacggtac cagtaaaggt
    48241 agtagtcatt gtttcctgag tatcaaatac gcaatcttgc agacgaatct ggaaactttt
    48301 cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg atatcaccca tatcaacttc
    48361 cagtttgtga tcgtccggaa cgatagaaca cactgagtct tcaatggtgc cgtaaaaacg
    48421 tgcagtaccg ttgttacccg cagcgattgt tgccgtagag gtgaacacgc ccatcaccat
    48481 tgccgcagca ata
//
LOCUS       DAFNBJ010000014        47377 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000014 DAFNBJ010000000
VERSION     DAFNBJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.014"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(859..1527)
                     /locus_tag="L2X49_000822"
                     /pseudo
     CDS             complement(859..1527)
                     /locus_tag="L2X49_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(1650..2102)
                     /gene="tabA"
                     /locus_tag="L2X49_000823"
     CDS             complement(1650..2102)
                     /gene="tabA"
                     /locus_tag="L2X49_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBN4795956.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(2247..2840)
                     /locus_tag="L2X49_000824"
     CDS             complement(2247..2840)
                     /locus_tag="L2X49_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN4795957.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTLLKE"
     gene            2911..3624
                     /gene="bdcA"
                     /locus_tag="L2X49_000825"
     CDS             2911..3624
                     /gene="bdcA"
                     /locus_tag="L2X49_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBN4795958.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3755..4150
                     /locus_tag="L2X49_000826"
     CDS             3755..4150
                     /locus_tag="L2X49_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBN4795959.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            4431..4565
                     /gene="pyrL"
                     /locus_tag="L2X49_000827"
     CDS             4431..4565
                     /gene="pyrL"
                     /locus_tag="L2X49_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBN4795960.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4569..5504
                     /gene="pyrB"
                     /locus_tag="L2X49_000828"
     CDS             4569..5504
                     /gene="pyrB"
                     /locus_tag="L2X49_000828"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBN4795961.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            5517..5978
                     /gene="pyrI"
                     /locus_tag="L2X49_000829"
     CDS             5517..5978
                     /gene="pyrI"
                     /locus_tag="L2X49_000829"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBN4795962.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            6051..6437
                     /gene="ridA"
                     /locus_tag="L2X49_000830"
     CDS             6051..6437
                     /gene="ridA"
                     /locus_tag="L2X49_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBN4795963.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(6643..9339)
                     /gene="mgtA"
                     /locus_tag="L2X49_000831"
     CDS             complement(6643..9339)
                     /gene="mgtA"
                     /locus_tag="L2X49_000831"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBN4795964.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(9480..9533)
                     /gene="mgtL"
                     /locus_tag="L2X49_000832"
     CDS             complement(9480..9533)
                     /gene="mgtL"
                     /locus_tag="L2X49_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBN4795965.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            9718..10665
                     /gene="treR"
                     /locus_tag="L2X49_000833"
     CDS             9718..10665
                     /gene="treR"
                     /locus_tag="L2X49_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBN4795966.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10784..12205
                     /gene="treB"
                     /locus_tag="L2X49_000834"
     CDS             10784..12205
                     /gene="treB"
                     /locus_tag="L2X49_000834"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBN4795967.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            12255..13910
                     /gene="treC"
                     /locus_tag="L2X49_000835"
     CDS             12255..13910
                     /gene="treC"
                     /locus_tag="L2X49_000835"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBN4795968.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLGYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGNAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            14304..16442
                     /gene="nrdD"
                     /locus_tag="L2X49_000836"
     CDS             14304..16442
                     /gene="nrdD"
                     /locus_tag="L2X49_000836"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBN4795969.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWSIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            16602..17066
                     /gene="nrdG"
                     /locus_tag="L2X49_000837"
     CDS             16602..17066
                     /gene="nrdG"
                     /locus_tag="L2X49_000837"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBN4795970.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPDKDIW
                     VWTGYKLDDLNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(17111..17497)
                     /gene="cybC"
                     /locus_tag="L2X49_000838"
     CDS             complement(17111..17497)
                     /gene="cybC"
                     /locus_tag="L2X49_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBN4795971.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDTLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(17552..18904)
                     /gene="pmbA"
                     /locus_tag="L2X49_000839"
     CDS             complement(17552..18904)
                     /gene="pmbA"
                     /locus_tag="L2X49_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBN4795972.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFEAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18998..19549
                     /locus_tag="L2X49_000840"
     CDS             18998..19549
                     /locus_tag="L2X49_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBN4795973.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19632..21005)
                     /gene="mpl"
                     /locus_tag="L2X49_000841"
     CDS             complement(19632..21005)
                     /gene="mpl"
                     /locus_tag="L2X49_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBN4795974.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESNFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            21181..22179
                     /gene="fbp"
                     /locus_tag="L2X49_000842"
     CDS             21181..22179
                     /gene="fbp"
                     /locus_tag="L2X49_000842"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBN4795975.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(22212..23207)
                     /gene="yjfF"
                     /locus_tag="L2X49_000843"
     CDS             complement(22212..23207)
                     /gene="yjfF"
                     /locus_tag="L2X49_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBN4795976.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(23194..24216)
                     /locus_tag="L2X49_000844"
     CDS             complement(23194..24216)
                     /locus_tag="L2X49_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4795977.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(24230..25732)
                     /locus_tag="L2X49_000845"
     CDS             complement(24230..25732)
                     /locus_tag="L2X49_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBN4795978.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADRGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25872..26828)
                     /gene="ytfQ"
                     /locus_tag="L2X49_000846"
     CDS             complement(25872..26828)
                     /gene="ytfQ"
                     /locus_tag="L2X49_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HBN4795979.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            27138..27668
                     /gene="ppa"
                     /locus_tag="L2X49_000847"
     CDS             27138..27668
                     /gene="ppa"
                     /locus_tag="L2X49_000847"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBN4795980.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(28048..28389)
                     /locus_tag="L2X49_000848"
     CDS             complement(28048..28389)
                     /locus_tag="L2X49_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBN4795981.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(28392..32171)
                     /gene="tamB"
                     /locus_tag="L2X49_000849"
     CDS             complement(28392..32171)
                     /gene="tamB"
                     /locus_tag="L2X49_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBN4795982.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDVGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(32168..33901)
                     /gene="tamA"
                     /locus_tag="L2X49_000850"
     CDS             complement(32168..33901)
                     /gene="tamA"
                     /locus_tag="L2X49_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBN4795983.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            34107..34745
                     /gene="msrA"
                     /locus_tag="L2X49_000851"
     CDS             34107..34745
                     /gene="msrA"
                     /locus_tag="L2X49_000851"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBN4795984.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            35068..36411
                     /locus_tag="L2X49_000852"
     CDS             35068..36411
                     /locus_tag="L2X49_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBN4795985.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36473..36679)
                     /locus_tag="L2X49_000853"
     CDS             complement(36473..36679)
                     /locus_tag="L2X49_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBN4795986.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            37004..37561
                     /locus_tag="L2X49_000854"
     CDS             37004..37561
                     /locus_tag="L2X49_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBN4795987.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(37551..38291)
                     /gene="cysQ"
                     /locus_tag="L2X49_000855"
     CDS             complement(37551..38291)
                     /gene="cysQ"
                     /locus_tag="L2X49_000855"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBN4795988.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNIMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            38481..40424
                     /gene="cpdB"
                     /locus_tag="L2X49_000856"
     CDS             38481..40424
                     /gene="cpdB"
                     /locus_tag="L2X49_000856"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBN4795989.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYISAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNNSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPDNTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMVNANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(40547..40927)
                     /locus_tag="L2X49_000857"
     CDS             complement(40547..40927)
                     /locus_tag="L2X49_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBN4795990.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            41016..41876
                     /gene="qorB"
                     /locus_tag="L2X49_000858"
     CDS             41016..41876
                     /gene="qorB"
                     /locus_tag="L2X49_000858"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBN4795991.1"
                     /translation="MIAITGATGQLGHYVIKSLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQAAYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSETDFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNK"
     gene            41985..42950
                     /locus_tag="L2X49_000859"
     CDS             41985..42950
                     /locus_tag="L2X49_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBN4795992.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            43058..43720
                     /gene="ytfE"
                     /locus_tag="L2X49_000860"
     CDS             43058..43720
                     /gene="ytfE"
                     /locus_tag="L2X49_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBN4795993.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43765..45177)
                     /gene="cycA"
                     /locus_tag="L2X49_000861"
                     /gene_synonym="dagA"
     CDS             complement(43765..45177)
                     /gene="cycA"
                     /locus_tag="L2X49_000861"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBN4795994.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(45486..46106)
                     /gene="fklB"
                     /locus_tag="L2X49_000862"
     CDS             complement(45486..46106)
                     /gene="fklB"
                     /locus_tag="L2X49_000862"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBN4795995.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLQGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            46325..46963
                     /gene="ytfB"
                     /locus_tag="L2X49_000863"
     CDS             46325..46963
                     /gene="ytfB"
                     /locus_tag="L2X49_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HBN4795996.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIILVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46947..>47377)
                     /locus_tag="L2X49_000864"
     CDS             complement(46947..>47377)
                     /locus_tag="L2X49_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN4795997.1"
                     /translation="KALDFSDLPPQKAIEKIIDFTWDYYIANPWFLKIVHSENQSKGV
                     HYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNINIAALGGYYLINQHT
                     LGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
BASE COUNT        11694 a        12534 c        11745 g        11404 t
ORIGIN      
        1 taatttttct aactgttttt cattaatata atcatatata ttgattttcc catcccgcag
       61 ggtttccatc tttcttgttc taatttttaa ttcattatcg atcttttctt caaaaatctt
      121 aacgacataa cccggaagtt tatttacaat ttcttttgtt ttttcttcat caccataaac
      181 atatcctaac tcattaagat acatctgttc tccctcataa ctcttaagta attgatagaa
      241 gtaccactta gcaaaatcag gttgcttcat gaaaaaatcc ttcaaaaaaa tatcattcac
      301 taaaatactg agaggcactt tatcaaaaac caatttaaca tcatcaaata gccttagtat
      361 taagcgcgat ttttcttgat tacttaaatt aaaatcattg acatatacaa ccaacttctc
      421 ccaaatcaat tcctgtaact cagggctgtt tttatagggt tctttattga ggttgaaaaa
      481 taatgtctca gctattttag actgatcgca gttaaaatca atatgatcga tcacggagcg
      541 ggcatgcttt atctttgtag tatcatcaga atattgcata ctttgattaa aacaccactc
      601 cagaattttt acatcattaa tatatggcaa ttttaaaaaa gtcttcaaca tcgaatcgat
      661 gaccttttcg agattaactt ttgcatcatc taaggaatca ataagaatat tagacaggtt
      721 aattttgatg ttattaattt catcattacc ttctgtacca ctagcaattc tggtatcatg
      781 taacgttttc agatcattta tttcttcaag tataccacct tcgcctagta agtatggaat
      841 cgtgtcatca catacatgag gcattaatag cagatgttct ttctttattt tttctaatgt
      901 actggctaat ggcttttcta ataactcgtt ataatcaatt gcgtccttcc agttcaactg
      961 atgattattt aatagaagcg ccatattctt gttagcttca cgttggtaac acgccagaac
     1021 cttgttagta atcaggtgta atgaactatc atcttttctt tcaaaagact taccttcata
     1081 acgaaaactc aaaaaatcag attgtggaaa tataatggct gtatgtcctt gacgagtccc
     1141 catagtagtg gtagtagaaa gtgtaatttc tatttccacc ttagataaat ccgctcgctc
     1201 cccttcagga ttattcataa caaacttcat tatctcacta ccatcattat ttttgatata
     1261 agatgaggac ttaatatgag ccatcatatt tctacccgct tcgttggagc gtttgctaaa
     1321 atccacataa cccagggtaa atatcttagc caaaaactcc aaaaatgatc tgggagaaag
     1381 aatattcctt ctgctatcct gccccccggc aatacgggca aaatcattca ttccaacacg
     1441 ccaggtattt aaaacttcac ttttttgttt aaaattgtcg gctaatgtaa tgtgttgaga
     1501 ataattcaaa tctgatattt tgctcatgac ggactccttg agttaataag aacgtctaag
     1561 cattacaaat tctactcaca aaaaaatatc aaaaaacgct cttttcataa gctcgccgta
     1621 aacctggagt tatttacggc gaaaagtcat cacgccatca gcatcttcac tactgctttg
     1681 cgaacccggg ctggcgcgcc cactgcgcac agcggtttat gcacttcccc cggataaaac
     1741 acaacaaaat caccttcatt taggataact gttttctcat caacgccttc cggcaaaaat
     1801 gcgatgtctt tatcagctaa ccagtcggta tccggcgtgc ctgcaggttg cgtgctgaag
     1861 gtcatgcctt cctgaccttt taacacaatc tgaatgtcga gatagcgggc atggtactcc
     1921 gcacggcgag cttcgtacgg ctcggtcata tcttccgaga taagataaaa cagacgatta
     1981 ccttcgatat cgtgcttgcc ctttggcgtt tctgccgtaa cgtgtgcttt gatatgctca
     2041 atcgcctggc gtaactcctg gggtagccac ggctgaagat tatgaatatt tccgatgatc
     2101 atcttttcca ctccttaatt aaaacaatgt ttcatttcca ccgttatacg aaaagatgat
     2161 caattccgcc agccttaacg cgtgaattat tgtcttatca caacttatcg gcaggcgcgt
     2221 tcccgccttt cggatcaagg ccagcatcac tccttgagaa gagtttttat cgcttccccc
     2281 gccagtgcag ctgttgcaca gagttgttcg agggagtgcc cctcccgtgc cttcgcagaa
     2341 agccctgaca tcacggtact cataaaatca gtcacacatt gtgcgctttg tggatgccgc
     2401 ctggcgatat agtcataaat ggtcgtttct gcggcgtgat aatattgaac ggcaatatca
     2461 cgcgctagtg gatcatgact atgaatacct tcaagaacca tacagccagc gcagccgccg
     2521 ttttggctat atctgcgcgc cgcttctttt aatacctcaa ccaggcactc gcctactgga
     2581 cgatcatcac gaagaatatc ggcaagcgga atagcttccg taccgacgta ttcattgagt
     2641 acacggctaa ataacccagc tttactgcca aaagccgcgt agaggctcgg ggggttaata
     2701 ccaagataat cagtaacttc agcaacactg acagcatcga aacctttttg gtgaaaaagc
     2761 acttttgccg cagagattgc ctgctcagga gcgaaacgtc tgggacgacc tggaacgcga
     2821 gattgttttt tagtgaccat tacaaaacct gttgacagaa agttaaaaca gttttgtaat
     2881 gcatgttaca taataaatca aggagtcctt atgggcgctt ttacaggtaa gacagttctc
     2941 atcctcggtg gcagtcgtgg tatcggtgcc gctatcgtac gtcgtttcgt caccgatggg
     3001 gccaatgtac gattcaccta tgcggggtcg aaagatgccg ctaaacgcct ggcacaagag
     3061 actggagcga cagcagtatt cacagatagt gctgacagag acgctgtcat tgatgtcgtt
     3121 cgtaagagcg gcgcattgga tatcctggtg gtaaatgcag gtattggcgt ctttggcgag
     3181 gccctggaat taaatgccga cgatattgat cgccttttca aaatcaatat tcatgctcct
     3241 tatcatgcct ctgttgaagc cgcccggcag atgcccgaag gcgggcgcat cttaatcatc
     3301 ggctccgtga atggcgatcg tatgcctgtt gcaggcatgg ctgcttatgc cgccagcaaa
     3361 tctgccctgc aaggcatggc gcgcgggctg gcccgtgatt ttggaccgcg tgggatcacc
     3421 attaacgtcg tccagccagg gccaattgat accgacgcta atcccgccaa cgggccaatg
     3481 cgcgatatgt tgcatagttt gatggctatc aaaagacatg ggcaaccgga agaggtcgct
     3541 ggtatggtcg catggttagc agggccagaa gccagttttg ttaccggcgc gatgcatacc
     3601 attgatggcg cgtttggcgc ataaccgact acgctcaatt aagcccagcc attatccatg
     3661 atgtctgggt tttgtttact cacgtcgtcc gctaaaagcg gctcccggta aagagaaatc
     3721 ttctgttcat gtcgtaaatc ttgaggataa ccaaatggta gaaagaaccg ctgttttccc
     3781 tgctggccga cattcactat atgctgagca tcgttattct gcggctattc gttccggcga
     3841 tttgctgttt gtttccgggc aagtaggaag tcgagaggac ggaacaccag aacccgattt
     3901 tcagcaacaa gtcagactgg catttgataa tttgcatgcg accctggcag ctgcgggatg
     3961 cacttttgac gatatcattg atgttacgag cttccatacc gatccagaaa accaatttga
     4021 agacatcatg acggtgaaaa atgaaatatt tagcgcccca ccttatccaa actggacggc
     4081 ggtgggtgtt acatggctgg caggctttga ttttgaaatt aaagtgatag cgcgcatccc
     4141 tgagcagtaa gcaatagtgt tagccgttcg ctttcacact ccgccctata agtcggatga
     4201 atggaataaa atgcatatct gattgcgtga aagtgaaaaa ggaaaaagca gggaatgtct
     4261 gcaattattg ataccgaagg acagttcccc tgcagaatca catcaaataa aaatgcatat
     4321 accttgactt ttaattcaaa taaaccgttt gcgctgacaa aatattgcat caaatgcttg
     4381 cgccgcttct gacgatgagt ataatgccgg acaatttgcc gggaggatgt atggttcagt
     4441 gtgttcgaca ttttgtctta ccgcgtctga aaaaagacgc tggcctgccg tttttcttcc
     4501 cgttgatcac ccattcccag cccctcaatc gaggggcttt tttttgccca ggcgtcagga
     4561 gataaaagat ggctaatccg ctatatcaga aacatatcat ttccataaac gaccttagtc
     4621 gcgatgacct taatctggtg ctggcgacag cggcgaaact gaaagcaaac ccgcaaccag
     4681 agctgttgaa gcacaaagtc attgccagct gtttcttcga agcctctacc cgtacccgcc
     4741 tctctttcga aacatctatg caccgcctgg gggccagcgt ggtgggcttc tccgacagcg
     4801 ccaatacatc actgggtaaa aagggcgaaa cgctggccga taccatttcg gttatcagca
     4861 cttacgtcga tgcgatagtg atgcgtcatc cgcaggaagg tgcggcgcgc ctggccaccg
     4921 agttttccgg caatgtaccg gtactgaatg ccggtgatgg ctccaaccaa catccgacgc
     4981 aaaccttgct ggacttattc actattcagg aaacccaggg gcgtctggac aatctccacg
     5041 tcgcaatggt tggtgacctg aaatatggcc gcaccgttca ctccctgact caggcgttag
     5101 cgaagttcga cggcaaccgt ttttacttca tcgcgccgga cgcgctggca atgccgcaat
     5161 acattctgga tatgctcgat gaaaaaggga tcgcatggag tctgcacagc tctattgaag
     5221 aagtgatggc ggaagtagac atcctgtaca tgacccgcgt gcaaaaagag cgtctggacc
     5281 cgtccgagta cgccaacgtg aaagcgcagt ttgttcttcg cgccagcgat ctccacaacg
     5341 ccaaagccaa tatgaaagtg ctgcatccgc tgccgcgtgt tgatgagatt gcgacggatg
     5401 ttgataaaac gccacacgcc tggtacttcc agcaggcagg caacgggatt ttcgctcgcc
     5461 aggcgttact ggcactggtt ctgaatcgcg atctggtact gtaaggggaa atagagatga
     5521 cacacgataa taaattgcag gttgaagcta ttaaacgcgg cacggtaatt gaccatatcc
     5581 ccgcccagat cggttttaag ctgttgagtc tgttcaagct gaccgaaacg gatcagcgga
     5641 tcaccattgg tctgaacctg ccttctggcg agatgggccg caaagatctg atcaaaatcg
     5701 aaaatacctt tttgagtgaa gatcaggtag atcaactggc attgtatgca ccgcaagcca
     5761 cggttaaccg tatcgacaac tatgaagtgg tgggtaaatc gcgccccagt ctgccggagc
     5821 gcatcgacaa cgtgctggtc tgcccgaaca gcaactgtat cagccatgcc gaaccggttt
     5881 cttccagctt tgccgtgcga aaacgcgcca atgacatcgc gctcaaatgc aaatactgtg
     5941 aaaaagagtt ttcccataat gtggtgctgg ccaattaatt gcggttggta ataaaagtct
     6001 ggctccctat aatgagccag actttttacc gctgtaataa aggagaaatc atgagcaaaa
     6061 ctatcgcgac ggaaaatgca ccggcagcta tcggtcctta cgtacagggc gttgatctgg
     6121 gcaatatgat catcacctcc ggtcagatcc cggtaaatcc gaaaacgggt gaagtaccgg
     6181 cagacgtcgc tgcacaggca cgtcagtcgc tggataacgt aaaagcgatt gtcgaagccg
     6241 ctggcctgaa agtgggcgat atcgttaaaa ccaccgtgtt tgtgaaagat ctgaacgact
     6301 tcgcaaccgt aaacgccact tacgaagcct tcttcaccga acacaacgcc accttcccgg
     6361 cacgttcttg cgttgaagtt gcccgtctgc cgaaagacgt aaaaattgag atcgaagcga
     6421 tcgctgttcg tcgctaatct tgatggaaat ccgggctatc atgcccggat taagtctgat
     6481 gacaaacgca aaatcgcctg atgcgctacg cttatcaggc ctacgtgatt cctgcaattt
     6541 attgaatttg ttggccggat aaggcattta cgccgcatcc ggcatgaaca aagcttactt
     6601 tgtctacaat ctgaatcggg gctatcgtgc ccagtttatt ctttattgcc agccgtaacg
     6661 acggctataa aatcctttca ccaactgggt taacgtcata taccctgcca gaatcgcaac
     6721 cagccacggg aaatagctta acggcagcgc ctgcagctgc agataactgg ccagcggtga
     6781 aaacggcaat gcgatcccga caatcatcac gatcacggtc atgatcatta acggccacga
     6841 tgcacagctc tgaataaacg gcacacggcg ggtacggatc atatgcacaa tcagcgtttg
     6901 cgacagtaag cccaccacaa accatcccga ctggaacagc gtttgcgttt ccggcgtgtt
     6961 ggcatggaat acccaccaca tcaggcaaaa cgtcagaata tcgaagatcg agctgatcgg
     7021 tccgaagaag atcataaagc gccccagatc cgccggattc caacgttgcg gcttttgaat
     7081 ttgctcgtcg tcgacgttat caaacgggat cgccacctgt gacacatcgt acagcaggtt
     7141 ctgaataagc aagtgtaacg gcaacatcgg caggaagggc aagaaagcac tcgctaccag
     7201 cacgctgaac acattaccga agttagagct cgccgtcatt ttgatgtatt tcagcatgtt
     7261 ggcgaaagtg cgacgtccct caataacccc ctcttccagc accatcaggc ttttttccag
     7321 caggatgata tcagccgctt cacgggctat atctaccgcg ccatccacag aaatgccgat
     7381 atccgcagcg cgtaaggcgg gcgcatcatt aataccatcg cccataaagc caaccacatg
     7441 cccttcgcgc ttcagtaagg tcacaatgcg ttctttatgc atcggcgtca gacgagcaaa
     7501 cagcgtggtg cgctgtgcga gatttgccag ttcgtcgtca gatagcgttt caatatcact
     7561 accaatgacc acctctcccg cgtccagccc cacttcatgg cacactttcg ctgcaactaa
     7621 ctcactgtcg ccagtaagga tttttacggt aattccactc gcttttaatg ccttcagtgc
     7681 cggagcagtt gtctcttttg gcggatcaag gaaagcaata tatccttcga ggatcaggtc
     7741 ggattcatcc gcccgctggt aatctccttc acgcgctggc aggtatttcg tcgccaccgc
     7801 aaccacgcgc agcccctgac gattcagcgt atcagtaacc cgcttaatct tacgcagcat
     7861 gatgtcatcg agcggcacaa tctcgccatt gtgacgcacc tgcgaacaca cattgaggat
     7921 ttcctgcaat gcacctttgc aaaccagctg atggtgctcg gtattttctg ccactaccac
     7981 cgacatccgg cgacgctcga aatcaaacgg aatctcatcg attttttgcc aacgactggc
     8041 cagcgagcgc gctgactctt catccgtacc ttcgagcacc gctgtatcga gcaggttttt
     8101 aagtccggtc tgataatgac tgttcaacca cgcgctatgc agcacgcgtt cgctggtttt
     8161 accggagata tcggtatgat tctccagcac aattttgtcc tgtgtcaggg tgccagtttt
     8221 atcagtgcac agaatatcca ttgcgccaaa gttctgaata gcatccagat gtttgacgat
     8281 cactttctgt ttcgacagct ttactgcgcc gcgcgccagc gtcgaggtaa caatcatcgg
     8341 caacatttcc ggcgttaagc ctaccgcgac cgaaagcgca aacagtgccg cttcccacca
     8401 gtcgcctttg gtgtaaccat tgattaacag caccaccgga gccatcacca gcataaagcg
     8461 aatcagcagc atactgacgc ggctgatccc ttgctgaaag gcattcggct cgctttcctg
     8521 ctcactaaca cgccccgcca gttgaccaaa ccaggtattg gcacctgtag caatcaccat
     8581 tgcttgtgcc gtgccgctca ccacggtggt gcccataaaa cacagggtgt cgcactccag
     8641 cggattgctg tgctccggct ggcgagtggt agctgctttt tctacgggca gagactcacc
     8701 ggttaacgac gcctgagcga cgaacagatc ccgcgcctgc aagatacgta aatctgccgg
     8761 gatcatatcc cccgccgcca gttttataat atcgccgggc accagctggt cgatcgggat
     8821 ctccagccag ccattttcgc ctttgtcgtt aattacgcgc agcaccgtcg cagtattgct
     8881 gaccatcgct ttcagggcat ctgccgcttt agtggaacgt gcttcctgaa taaagttcag
     8941 caacgtagaa atagcgacca ttagcgcgat aacgcccgcg gcaaataaat cttccgtggc
     9001 gtaagaaata gcgccgagaa tggtgagtaa aatattaaat gggttgcgat agcagaccca
     9061 taaatgtacc caccacggcg acggttgttg tgcgggtaat ttattttcac catgttgttc
     9121 gcgggcagat tccacttccg cctgatttaa cccttccgga tgggtgtcga acgttttcca
     9181 taattcttct tcgggcatca ccgccatttt caggcaatgc gcacttaagg acggcgggac
     9241 cgccgtattc actgtctgct gcgcgccagg caatggatcg cgatgaacca gacgggaagg
     9301 taaatggcga atgagccggg taaaaatttc tttaaacata aggagtccct ccgcactgtc
     9361 tgaaacagta cagaaatgtc ttcaggcgtg gcgaagcctt acctgaaggt aataaagctg
     9421 agcagggacg ttaaaacgtc acggccttac gcatccggta acgccgtgaa aagacgggct
     9481 taccggaaaa gttttaatct ccgtcgaggg agaggcgtgg gatcaggttc catataacct
     9541 ccggtaagtg aaaaaagcgc cgcaaataat aaggagaatc caataatatt cgtagcatct
     9601 atggcgatat aatacctgct ggcaactaaa ccaacgataa accagacttt acccattgct
     9661 gaatgcacgg gtaaagttag gctcaaataa ttaaacaaca cgttacagga caacaggatg
     9721 caaaatcggc tgaccatcaa agacatcgca cgcttaagcg gcgtggggaa atctacagtt
     9781 tcccgggtgc tgaataacga aagcggcgtg agccagcgca cccgcgagcg tgttgaagca
     9841 gtgatgaatc agcatggatt ttccccttcc cgctctgcgc gcgctatgcg tgggcaaagc
     9901 gataaagtgg tcgccatcat tgttacccgt ctggattcgt tgtcggaaaa tctcgccgtt
     9961 caaaccatgc tgccagcgtt ctatgaacaa ggttacgacc caatcatgat ggaaagtcag
    10021 ttttccccac aattagttgc cgaacatttg ggggtgctga aacggcgtaa tatcgacggc
    10081 gtagtgctgt tcggttttac tggcataaca gaagaaatgt tagcccactg gcagtcatcg
    10141 ctggttctgc tggcgcgtga cgcaaaaggc tttgcttcgg tctgttatga cgacgaaggg
    10201 gcaatcaaga tcctgatgca acggctgtat gaccaggggc atcgtaatat cagttatctc
    10261 ggcgtgccgc atagtgacgt gacaaccggt aagcgacgtc acgaagccta cctggcgttc
    10321 tgcaaagcgc ataaactgca tcccgttgcc gctctgccag ggcttgctat gaagcaaggc
    10381 tatgagaacg tggcaaaagt gattacgcct gaaactactg cgttactgtg cgcaaccgac
    10441 acgctggcac ttggcgcaag taaatacctg caagagcaac gcatcgacac cttgcaactg
    10501 gcgagcgtcg gtaatacacc gttaatgaaa ttcctccatc cggagatcgt caccgtagat
    10561 cccggttacg ccgaagctgg acgccaggcg gcctgccaat tgatcgcaca ggtaaccggg
    10621 cgcagcgaac cgcaacaaat catcatcccc gccaccctgt cctgatcgtt tcctgaacga
    10681 taaattgtga tcttcgctgc gtttcgggaa cgttcccgtt tttaaatttt tccgcgcaat
    10741 atattctgca gccaaccaaa aatgtcatct gccatggggc tttatgatga gcaaaataaa
    10801 ccaaacggat atcgatcggt tgattgaact ggtcggcggg cgcggcaata ttgcgacagt
    10861 gagccactgt attactcgcc tgcgctttgt cctcaaccaa ccggccaatg ccagaccgaa
    10921 agaaattgag caactcccca tggtgaaagg ctgtttcacc aatgccgggc aatttcaggt
    10981 ggtgattggc accaatgtgg gtgattacta tcaagcacta atagcgtcaa ccggacaggc
    11041 gcaggttgat aaagagcagg taaaaaaagc cgcccggcag aatatgaaat ggcatgagca
    11101 gttgatctct catttcgcgg agatcttctt cccgttgctg cccgcgttga ttagcggcgg
    11161 tttgatcctc ggttttcgca atgtgatcgg cgatttgccc atgagcaacg gtcaaacgct
    11221 ggcgcaaatg tacccttccc tgcaaacgat ctacgatttt ctgtggttga tcggtgaagc
    11281 gatcttcttc tacctgccgg tcgggatttg ctggtcagcg gtgaaaaaaa tgggcggcac
    11341 gccgatcctc ggtatcgtgc ttggcgtgac actggtttct ccacagctga tgaacgctta
    11401 tctgctcggg cagcagctgc cggaagtgtg ggactttggc atgttcagca tcgccaaagt
    11461 aggctatcag gcgcaggtga tcccggcact gttagccgga ctggcgctgg gcgttattga
    11521 aactcgcctt aaacgcatcg tgccggatta cctctatctg gtggtggtac ccgtctgttc
    11581 gctgatcctc gcggtgttcc tcgcccatgc gctgattggt ccgtttggtc gcatgattgg
    11641 cgatggcgtt gcctttgcgg tacgtcacct gatgaccggc agctttgctc cgattggtgc
    11701 ggcattgttt ggcttcctgt acgcgccgct ggtgatcacc ggcgtacacc agaccaccct
    11761 tgctattgat ttgcagatga ttcaaagcat gggtggcacg ccagtgtggc cgctgattgc
    11821 gctgtcgaat atcgctcagg gctccgccgt gataggcatt atcatttcca gccgcaagca
    11881 caatgaacgc gagatctccg tgcctgccgc tatctccgcc tggcttgggg tcactgagcc
    11941 tgcaatgtac ggcatcaacc tgaaatatcg cttcccgatg ctgtgcgcga tgattggttc
    12001 tggtctggca ggattgctat gcggcctgaa cggcgttatg gcgaatggca tcggcgtagg
    12061 cggcctgccg ggaattctct cgattcaacc gagctactgg caggtgtttg cgctggcaat
    12121 ggctatcgcc atcatcatcc cgattgtact cacctcgttt atctatcagc ggaaataccg
    12181 cctgggcacg ctggacattg tttaattttc ttcggggcgc aattgcgccc tctcgcattt
    12241 gcaggaataa cgtaatgact aatcttcccc actggtggca aaacggcgtt atctaccaga
    12301 tttatccaaa gagttttcag gacaccacgg gtagcggcac cggcgattta cgtggcgtta
    12361 tccaacgcct gggctatctg cataaactgg gcgttgatgc catctggcta accccctttt
    12421 atgtctctcc ccaggtcgat aacggttacg acgtagcgaa ctatacggcg attgatccca
    12481 cttacggcac gctggacgat tttgacgaac tggtgacgca ggcaaaatcg cgcgggattc
    12541 gtatcattct cgatatggtg tttaaccata cctctaccca acatgcctgg tttcgcgagg
    12601 cgctgaacaa agaaagccct taccgccagt tttatatctg gcgcgatggc gaaccagaaa
    12661 cgccaccgaa caactggcgt tcaaaatttg gcggtaatgc gtggcgctgg catgcggaaa
    12721 gcgaacagta ctatttgcat ctctttgcac cagagcaggc ggatctcaac tgggagaatc
    12781 cagcggtacg cgcagagctg aaaaaagtct gtgagttctg ggccgatcgt ggggtcgacg
    12841 ggttgcgcct ggatgtggtg aatctgatct ccaaagaccc acgtttccct gatgacctgg
    12901 atggcgacgg gcgtcgcttc tacaccgacg ggccacgagc acacgagttt ttgcacgaga
    12961 tgaaccgcga tgtgtttacg ccacgcgggt taatgaccgt aggtgaaatg tcctccacca
    13021 gccttgagca ttgccagcga tacgcggcac tgacaggcag tgaattgtcg atgaccttta
    13081 attttcatca cctgaaggtc gattatcccg gtggtgaaaa atggacgctg gctaaacctg
    13141 actttgtggc gttgaaaaca ttgttccgcc actggcaaca aggaatgcac aacgtggcat
    13201 ggaatgcctt gttctggtgt aaccacgatc agccgcgcat tatttctcgc tttggtgatg
    13261 aaggtgaata ccgcgtgcct gcggcaaaaa tgctggcgat ggtgctgcat ggcatgcagg
    13321 gaacgccgta tatctaccag ggcgaagaga ttggcatgac caacccgcat ttcacgcgca
    13381 ttactgacta tcgcgacgtg gagagcctca atatgtttgc cgagctgcgc aacgatggtc
    13441 gtgatgccga cgagttattg gcaatcctcg ccagtaaatc ccgtgacaac agccgcacgc
    13501 ccatgcaatg gagcaacggc gataatgccg ggtttacggc tggcgaaccg tggattggcc
    13561 tgggcgataa ctatcaacaa atcaacgtag aagccgcgct ggccgatgag tcctcggtgt
    13621 tttacaccta ccaaaagtta atcgcactgc gtaagcaaga agccatcctg acatggggca
    13681 attaccagga tctgctgcca aacagccctg tattgtggtg ctatcgccgt gaatggaagg
    13741 ggcaaacctt gctggtcatt gccaacctta gccgtgagat ccaaccctgg cagccagggc
    13801 aaatgcgcgg caactggcag cttgtgatgc ataactacga agaagcctca ccacaaccct
    13861 gtgccatgaa tttacggcct tttgaggctg tctggtggtt acagaagtaa atcttcccta
    13921 agccccggta atgccggtca ttccggggtt ttgtctgtca gcatgttgtt tttgttgatt
    13981 tttcaaccag caaattcatt aaaaaattta cataacgctg tagcgcccgt catccgtacg
    14041 ctctgctttt tactttgagc tacatcaaaa aaagttaaaa catccttgat gcaaagcact
    14101 atatatagac tttaaaatgc gtcccaaccc aatatgttgt attaatcgac tataattgct
    14161 actacagctc cccacgaaaa aggtgcggcg ttgtggataa gcggatggcg attgcggaaa
    14221 gcaccggaaa acgaaacgaa aaaaccggaa aacgcctttc ccaatttctg tggataacct
    14281 gttcttaaaa atatggagcg atcatgacac cgcatgtgat gaaacgagac ggctgcaaag
    14341 tgccgtttaa atcagagcgc atcaaagaag cgattctgcg tgcagctaaa gcagcggaag
    14401 tcgatgatgc cgattattgc gccactgttg ccgcggttgt cagcgagcag atgcagggcc
    14461 gcaaccaggt ggatatcaac gagatccaga ccgcagttga aaaccagctg atgtcgggtc
    14521 catacaaaca actggctcgc gcttacatcg agtaccgtca cgatcgcgac attgaacgtg
    14581 aaaaacgcgg tcgcctgaac caggagatcc gtggtctggt cgagcagacc aacgcctcgt
    14641 tactcaacga aaacgccaac aaagacagca aggtgattcc aacccagcgc gacctgctgg
    14701 ccgggatcgt ggctaaacac tatgcacgtc agcacctgct gccgcgtgac gtggtgcagg
    14761 cacatgagcg tggcgatatt cactatcacg atctcgatta ctcaccgttt ttcccgatgt
    14821 tcaactgcat gttgatcgac ctgaaaggca tgctgaccca gggctttaaa atggggaacg
    14881 ccgagattga accgccgaag tcgatctcaa cggcaaccgc ggtaactgcg cagattattg
    14941 ctcaggttgc cagccatatt tatggcggca ccaccattaa ccgtatcgat gaagtgctgg
    15001 caccgtttgt caccgccagc tacaacaaac atcgcaagac cgcagaagag tggagcatcc
    15061 cggacgcaga aggctacgct aactctcgta ccattaaaga gtgctacgat gccttccagt
    15121 cgctggagta cgaagtaaac accctgcata ccgccaacgg tcaaacgccg tttgtaactt
    15181 ttggttttgg tctgggcacc agctgggaat cgcgcctgat tcaggaatct atcctgcgta
    15241 accgtatcgc aggtctcggt aaaaaccgta aaactgcggt gttcccgaaa ctggtgtttg
    15301 cgattcgcga tggtctgaac cataaaaaag gcgatccgaa ctacgacatc aaacagctgg
    15361 cgctggagtg cgcaagcaag cgcatgtatc cggatatcct gaactacgat caggtagtga
    15421 aagtcaccgg ttcgtttaaa accccgatgg gctgccgcag cttcctcggc gtgtgggaaa
    15481 atgaaaacgg cgagcagatc cacgatggtc gtaacaacct cggcgtgatc agcctgaacc
    15541 tgccgcgtat tgctctggaa gcaaaaggcg atgaagccac cttctggaag ctgctggatg
    15601 aacgtctggt gctggcacgt aaggcgctga tgacccgtat cgctcgtctg gaaggcgtga
    15661 aagcgcgcgt ggccccgatc ctttatatgg aaggtgcttg tggcgtgcgt ctgaatgctg
    15721 acgatgatgt ttctgaaatc ttcaaaaacg gtcgtgcgtc tatttcgctg ggttacatcg
    15781 gcatccacga aaccattaac gcgctgttcg gcggcgagca tgtttacgac aacgagcagc
    15841 tccgcgcgaa aggtatcgcg attgttgaac gtctgcgtca ggcagtggat cagtggaaag
    15901 aagaaacggg ttatggtttc agtctctaca gcacgccgag tgaaaacctg tgcgaccgct
    15961 tctgccgtct cgatactgct gagtttggcg tggtgccggg cgtgaccgat aaaggttact
    16021 acaccaacag tttccacctt gatgtggaga agaaggtgaa cccgtacgac aagatcgact
    16081 ttgaagcgcc ttacccgccg ctggcgaacg gtggtttcat ttgctacggc gagtatccaa
    16141 acattcagca caacctgaaa gcgctggaag atgtctggga ttacagctat cagcatgtac
    16201 cgtattacgg caccaataca ccgattgatg agtgctacga gtgtggcttt accggtgagt
    16261 tcgagtgcac cagcaaaggc ttcacttgcc cgaaatgtgg taaccatgac gcctcccgtg
    16321 tgtcggtaac tcgccgcgtg tgcggatatt taggtagccc ggatgcacgt ccgtttaacg
    16381 ctggtaagca ggaagaagtt aagcgccgcg ttaaacattt ggggaatggg cagataggtt
    16441 aatcttcact ctgcgctaag caaatgccgg atgcggcgta aacgccttat ccggcctaca
    16501 tttggcaacg gctgtgtagg cctgataaga cgcgacagcg tcgcatcagg cattgattac
    16561 cggatgcagc ctacacgcgg actgataagc caggcgaaat aatgaattat catcagtact
    16621 atcctgtcga catcgtcaac ggccccggca ctcgctgcac cctgtttgtc tccgggtgtg
    16681 ttcatgaatg ccccggttgc tataacaaaa gcacatggcg ggtaaattct ggtcagccat
    16741 ttaccaaagc aatggaagac cagatcatta acgatctgaa tgacactcgt atcaaacgcc
    16801 aggggatttc cctctccggc ggcgatccgc tgcatccgca aaacgtgccg gatattctga
    16861 aactggtaaa acgcatccgc gccgagtgtc cggataaaga catctgggtg tggacaggct
    16921 ataaactcga cgatctcaac gctgcgcaaa tgcaggttgt tgatttgatt aacgtactgg
    16981 tcgacggcaa atttgtgcag gatttaaaag acccttccct gatctggcgc ggcagcagca
    17041 atcaggtggt gcatcatttg cgatgataag ttccctgtta cctgagtgca ggcaacaggg
    17101 aaatgaggaa ttaacgatac ttctggtgat aggcgttgcg ggtcgttttc agttgctctg
    17161 cggcagcctg cgcttctttt actttacctt catttgccag cttcagcgcg tcgtcaatct
    17221 gaccaaccag aatatcgaaa ccgtgacgga agtctttcat ttccgggctg tccggtgatt
    17281 tatcttcgag cttcggcggc gttgcttttt gcgcatccag cgctgcggcg cgcatcttcg
    17341 ttaacgcgtc tttgacttgc gccgcgttat cggctttttc gaccactttt aaagtgtcgt
    17401 tgagggtttc catattgtct tcaagatcag cggcaaacga cgccgaactg aataccaacg
    17461 aggagactgc aagaatagct aacaggcttt tacgcattgc tcacttcctt ttttattatg
    17521 aagatttggc gcggcaggtc gcgccagttt tttactgccc ggcgattttc atctccggca
    17581 acagcacaga accacactgt atattactgc gtgtttcaat atcgttaccg acggtgacaa
    17641 tattgcgcca catatctttt aaattacctg cgatggtgat ttcgctaacc ggatactgaa
    17701 tttcgccgtt ttctacccag aagcccgccg caccacggga ataatcaccg gtaattgcac
    17761 tcacgccctg gcccatcagc tccgtcacca ccagcccggt acccatctct ttgagcattt
    17821 gttcgaagct tagcccttgt ccggcaatcc gccagttgtg aataccgccc gcatgtccgg
    17881 tgcttttcag ccccagtttt cgcgccgaat agctggtcag cagccactga gtcaggatgc
    17941 catctttaat aatatcgcga cgctcggtgc gcacaccttc gctgtcaaat ggcgtcgatg
    18001 ccagcccttt cagcagatgc ggatgctctt caatggtcag ccagtccggc agaatttgtt
    18061 tacccagcga atccagcagg aaggtagatt tacggtaaac cgctccaccc gctatcgccc
    18121 ccaccagatg cccaaaaagc ccggttgcca cttcattggc aaaaatgacc ggcgctttca
    18181 tggtggagag tttacgcggt gacaggcgcg ataaagtacg gcgagcacag tcggccccaa
    18241 cccactctgg cgtttgcaga tcgctcatcg cacgaccaat ggtgtaggcg taatcacgct
    18301 ccatatcgcc attttcttcg gcaattacac agctggagag cgaatgacgc gtagagcagt
    18361 aaccctgcaa catgccgtgg ctgttgccaa aaactttgac accgtagtgg ctgttaaagc
    18421 tgccaccttc ggtattggtg atacgtttgt ccgcctgtaa tgctgcctgt tctgcgcggg
    18481 ccgccagttc aatggcttca tccggggaaa cttccgcagg gtggaacaaa tcgagatccg
    18541 gtgcctcaaa ggccagcagc tctttgtctg ccacgccggc acagggatct ggcgaggtat
    18601 aacgggcaat atccagcgcc gcctgtacgg tgcgggcaat ggcctgcggg cttaaatcgg
    18661 tggatgatgc gctacctttg cggttctggt gataaacagt gatccccagc gcgccatcgc
    18721 tattgaattc aacattctcc acttcaccat aacgcgtgct tacgctaatg ccggtggtct
    18781 tgctgacggc aacttccgca ccgtccgatt tgcctgaggc caactccagc gcagtggaaa
    18841 ctgcttcttc cagaatcttg cgctgcgctt caacttgaga gattactttc attgcaagtg
    18901 ccataatgta gagagtttct ctgaagtcta acagagaacc gtttttcagt gcgcatctta
    18961 actggtaaca ttagcctctt tttttaagga gcctgagatg actaagcagc ccgaagactg
    19021 gctcgacgac gttcccggtg atgacatcga agacgaagac gatgaaatta tctgggtcag
    19081 taaaagtgaa attaaacgtg atgccgagga gctaaaacgc cttggcgcgg aaattgttga
    19141 tctggggaaa aacgcgctgg ataagatccc gttagatgcg gatctgcgcg cggctattga
    19201 gctcgcccag cgtattaaga tggaaggtcg ccgccgccag ttgcagctca tcggtaagat
    19261 gctgcgccag cgcgatgtag aaccgatccg tcaggcgctg gataagctga aaaaccgtca
    19321 caaccagcag gtggtgctgt tccataaact ggaaaacctg cgcgatcgcc tgatcgatca
    19381 aggtgatgac gccatcgctg aggtattaaa tctgtggccc gatgccgatc gtcagcaact
    19441 gcgtactctg atccgtaacg cgaagaaaga gaaagaaggg aataagccgc cgaaatccgc
    19501 acgccagatt ttccagtatc tgcgcgaact ggcggagaat gaagggtaat tttcgttgtt
    19561 gatgtgagta tggtttgccg gatgcggcgt aaacgcctta tccggcaatg ctatctcagg
    19621 ctgaggccga attactgcgc agcttccgcc ttcttcgcca gaccatccag cagtttctga
    19681 tggatcccac caaaaccgcc gttgctcatc accagaatat ggtcgccagg ctgagcggtt
    19741 ttcaccacca tatctgccag cgtatccaca tcgccactcc agtgtgcagg ctgaacgcag
    19801 gcttctgcca cttctgccac ctgccacgga atatgcgccg gttgcagcag gaagacttca
    19861 tcggcacgac ctaatgaagg tgccagatcg tctttgcaga tccccatttt catggtattc
    19921 gagcgcggtt ccagcacagc aataatgcgc gccgtaccac caactttgcc acgcagcgcc
    19981 gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat cgtcatatac cgtgacgcca
    20041 ttcgcttcac cacgcaactc cagacggcga cgagcattaa taaacgaacc cagcgcgtta
    20101 gcggcatctg ccggcgctac accaacatgg cgagccgctg caatcgccat caggccattg
    20161 tgcatattat gttcgcctac cagcgaccat ttcacttcgc ccactttttc gccatccagc
    20221 aaaacttccc attcggaagc atcggtggtc agctttttcg cctgccagtg accctgctcg
    20281 cccaccagct cctgctcgct ccagcagccc atcgccatgg tctgtttcag gttgatgtcg
    20341 ttttctggcc agataatacg gccctgccct ggaacgatac gcaccagatg gtggaactgt
    20401 ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat cgaactcaag gttgttgagg
    20461 atcagcgtac gcgggcagta atgaacaaat ttagagcgtt tgtcgaagaa ggcgcagtca
    20521 tactcatccg cttcgataac aaagaagttg ctttcgccca gacgcgccga aacctcaaag
    20581 ttccccggca caccgccgat cacaaatccc ggtttgtaac cgcactgttc cagaatccag
    20641 gtcgccattc ccgcggtggt ggttttgcca tgtgtaccgg caacggccag cacccagcgg
    20701 tcgcgcagca caaaatcgtg cagccactgt ggacctgaca tataagggat gtttttttcc
    20761 agtaccgctt ccacacacgg atttccacgg gtcatggcgt tgccaataat caccagatcc
    20821 ggctgcggat cgagctggct ggcatcgtaa ccctgaatca gttcaatgcc ttgcttctca
    20881 agtaaggtgc tcatcggcgg atacacattg gcgtccgaac ccgttacttc atggcctaac
    20941 tggcgcgcca gcatcgccag accgcccata aacgtgccac aaattcctaa aatatgaatg
    21001 cgcatacgtc actatccttt tttaatctgc cgctcatttt acgcatatgt cgggcaagtg
    21061 agaaacgcat ttcaggataa tccgcaattt gctggcgcga ttcacctgag cgcaacattc
    21121 cgattatttg ttaagattgt tacggtcgct ttactccata aacattgcag ggaaagtttt
    21181 atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg agttttctca tgctaccggt
    21241 gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca agattatcca tcgcgatatc
    21301 aacaaagcag gactggttga tatcctgggt gccagcggtg ctgagaacgt gcagggcgag
    21361 gttcagcaaa aactcgactt gttcgctaat gaaaaactga aagccgcact gaaagcacgc
    21421 gatatcgttg cgggcattgc ctctgaagaa gaagatgaga ttgtcgtctt tgaaggctgt
    21481 gaacatgcaa aatacgtggt gctgatggac ccactggatg gctcgtccaa catcgatgtt
    21541 aacgtctctg tcggtaccat tttctccatc taccgccgcg ttacgcccgt cggcacgccg
    21601 gtaacggaag aagatttcct ccagcctggt aacaaacagg ttgcggcagg ttacgtggta
    21661 tacggctcct ctaccatgct ggtttacacc accggatgcg gtgttcacgc ctttacttac
    21721 gatccttcgc tcggcgtttt ctgcctgtgc caggaacgga tgcgcttccc ggagaaaggc
    21781 aaaacctact ccatcaacga aggaaactac attaagtttc cgaacggggt gaagaagtac
    21841 attaaattct gccaggaaga agataaatcc accaaccgcc cgtatacctc acgttatatc
    21901 ggttcactgg tcgcggattt ccaccgtaac ctgctgaaag gcggtattta tctctaccca
    21961 agcaccgcca gccacccgga cggcaaactg cgtttgcttt atgagtgcaa cccgatggca
    22021 ttcctggcgg aacaagcggg cggtaaagcg agcgatggca aagagcgtat tctggatatc
    22081 atcccggaaa ctctgcacca gcgccgttcg ttctttgtcg gcaacgacca tatggttgaa
    22141 gatgtcgaac gctttatccg tgagttcccg gacgcgtaat ctcctgcgcc tctccacacg
    22201 gagaggcgtt tttatcgctg cgcaatgttg actcttgtca ctggcgaact ctgacgattc
    22261 tcccacagca ccgttaatcc acgctgtaat gctataaaaa taaacaacaa aataccgatg
    22321 gcgattttcg tccaccatga gctcagcgtg ccatcaaagt ttatgtaagt ctgaatcagt
    22381 ccctgaatcg ccacgccaaa aagcgtccct aataccgttc caacgccacc gctcagaagc
    22441 gtaccgccaa ttaccactga ggcgatagca tccagttcca cacctacgcc cgccagcgca
    22501 tatccggcct gggtataaat cgagaagaca atccccgcca gcgttgccag tccggtggag
    22561 agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc ccatcaggtt cgccgacgtt
    22621 gcgttgccgc caatggcgta tacctgatta ccaaaacggg tacgatgcgc gaggaaaata
    22681 ccgataacca ccaccgccaa catcagcagt cccatcgcac ttaagcgacc gccgccaggg
    22741 attttccacg caaggcttga gagcgtgtca taaatgggat ggtttatcgg aatcgactct
    22801 tccgaaacga gatagctgac accgcgcaaa aagaacatcc ccgccagggt aatgataaat
    22861 gccgggatct tcagggcgtc gatcaaaagc cccataaatg cgccgaaggc acagcccatc
    22921 accagcacta gcggaaacgc cagtagcggc gagaggccga aatcgccaat cacttttgcc
    22981 agaaacacgc cagtaaaggc gatcaccgaa ccgacggaga gatcgatccc accggagagg
    23041 atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg cgttatcggt caggatattg
    23101 cagatcactc ttgtggaagc aaaaccggga aactgggtca ggcagtaaag ataacccaac
    23161 acaaagacgc cgatggtgat catcagtggc aaattacgtt ttatcacggc tacgtactcc
    23221 tttaatcaga ctgataaagc gttgcgactg gacaatcagc acgcaaagaa ccaccaccgc
    23281 ttttacaacc tggttcatct ctggcggaaa gcccgaaagc aaaattccgg tgttcatccc
    23341 ctgaataatc agcgccccca ccaccgaaag cagtaggtta aaacgtccgc ccatcagcga
    23401 tccgccgcca ataaccaccg cgagaatggc gtccagctcc agccataacc cggcgttatt
    23461 ggcatcggca ccgcgaatat ccgccgccac gataatgccc gcaatcgccg cacacagccc
    23521 gctcaacacg taggtaagca tgacgatgat ccgcgtgttt accccggcat tttttgccgc
    23581 ccgaatgttg ataccaacgg cttcgataaa catccccagc gccgttttgc gggtcaacag
    23641 ccagaacagg ataagcgtca gcaccgcgat aatgaccggc gttggcagga acaacagcga
    23701 tccactgcca aaccatgaga gatccggcga gttaaacgtg acgatctgtc cggaggtgat
    23761 cagttgcgcc acgccgcgcc cggcgaccat caggatcagg gtggcaacaa acggctgaat
    23821 tttgaggatc gctaccagta tgccgttcca caatcccgcc aggatgccag tgcccagggc
    23881 gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc atcgcagccg ttgtggctcc
    23941 ggcgatagcc atcaccgccc ccacggagag atcaatccca cctgttgcga tcaccagcgt
    24001 cattccgata gccaacagcg caacgggggc cgcacggtta agaatgtcta tggggctacc
    24061 gaataaacgc ccatcctgga gcaccacctg ccagaaatgc ggggccacca ggctatcgac
    24121 cagcagcacc agcaacagtg ccgccagctg cggcattccc gttggccagc gaaagcgcct
    24181 tttcggcggc gtagtgtccg ggagagattg aggcatcaca ctgttctcct tacgccgcaa
    24241 tggcgttcat gatcgccgga acggaaagct ctgccagcgg gatctccgcc acctgtttgc
    24301 gatcgcgcat gatgatcacc cggtcggcat agcccaccag ttcttccagt tcggaggaga
    24361 tcaccagcag cgccagacca tcggcgcata gcgtttcaat caggcggatg atctcggcgt
    24421 gggcaccaac atcaatgccg cgagttggct catcaaggat cagaaattgc ggtcgggtca
    24481 gtagccaacg tgaaagcaac actttttgct gattgccgcc agagagaaat tcaatcggtt
    24541 gttcagttga aggtgtgcga atgccaagct ggcggataaa gcgttcggca atctcttgct
    24601 gttctttgcg ggaaatggga cgtagccagc cgcgctgggc ctggagagcg aggatgatat
    24661 tttcccgcac cgaggcggca gcgatgatgc catcggtttt cctgtcttcc gggcagaagc
    24721 caatgcccag taccgaagcc tgatgtggcg atcgcaggtt ttgcggtttg cctttgatca
    24781 acgccgtgcc gcggtcagca ggtttgatac cgaagatcac ttcggcggtt tcggtacgtc
    24841 ctgatcccag caatccagcc agaccgacga tctcgccggg gcgtacttcg agatcaaacg
    24901 gtgcgatcgt tccttttttg ccgtaatttt tgaacgcggc aacgggtttg tcgctcaaca
    24961 atgttcgccc ggcacgctgt agcgcgtggg tatccagctc gcgccccagc atcattttta
    25021 ccagttcgat ctgcggtagc tcgcacgttt cccgacagcc tacgaaactg ccgttgcgta
    25081 agacggtgat ccgatcgctg acctgataga cctgatcgag aaagtgagtg acaaaaatca
    25141 ggctgacgcc gcgatcgcgc aactgacgca tcaggtcaaa cagtaactcc acttcctggg
    25201 tgtcgagact ggcggtgggt tcatcgagga tcagcacttt ggcagagaga tcgatagccc
    25261 ggcaaatagc gacgatttgc tgcatcgcga ctgaaaagcg gttgagcggt tcgcgcacgt
    25321 cgagggagaa accgtaagat gccatcagtt cggtggcgcg cttttccatc tctttgcggc
    25381 gtagaaggcc gaagcgtttg ggttcgcggc ctataaatag attatcagcg accgacatat
    25441 tgggtagcag gtttacttcc tgatagacgg tgccgatacc gagttgttgt gcatgggcgg
    25501 tatttttcgg tgagatagcc tggccttcca gccagatggt gccgcgatcg gcgtggtata
    25561 caccagttaa tgctttgatt agcgttgatt ttcctgcccc gttttcaccg agcagcgcca
    25621 tgatttcgcc gcggcgcagg ctgaaatcaa cgttgtctaa cgctttgacg ccggggaaaa
    25681 atttacttaa tccttcggtg cggaggatct cctggtgttg gttagtcgtc atggtttccc
    25741 ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg gtaagcgtcg catcaggctt
    25801 cagcgcacca ccgccggatg cggcgcgagc gccttatccg gcctacgaat cggcatcagg
    25861 catagcaacc ctcaataccc catatttttc ttcttctcta actcttcttt tgcggtatca
    25921 ggcagataaa gggtggattt ggtcagcgtc agcttttcag gcatggtgcc gtcttttttg
    25981 tatttctcca gcgcgtcgaa ggcgggacct gccatattcg gcgtcagttc aacactggcg
    26041 ttcgcttcgc catcaatcat cgctttgtag atatccggta cgccgtcgat agaacctgtc
    26101 agaatatctt tgcctggttt caggcccgct tctttaattg cctgaattgc accaatcacc
    26161 atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc cgttgttttc cgctttgata
    26221 aagctctcca tgacttcttt gcctttactg cgggtgaagt cacctgactg cgagcggata
    26281 attttgatat ttggcgcatt cttaatggct tcggcaaagc ctttcttacg gtcaatggcg
    26341 acgctggccc caacagtgcc ctgcagctcc accacgttgc atggtttgcc attcacttct
    26401 tttaccagcc agtcaccaat caacttgcct tcgagaatgt tgtcggcagt gacggtggtc
    26461 atatagagag atttgtcttt cacatcaata gaacgatcga gcaagaagac cgggatttcg
    26521 gcatctttcg cctcttttaa taccggttcc caaccagtcg caaccaccgg agcgataaag
    26581 atcgcatcca ccccttgcgc gacgaaggaa cgtaccgctt taatctggtt ttcctgcttt
    26641 tgctgaccat cggcaatttt cagcgtaatt ccgcgttttt cggcctcact tttcgccaca
    26701 ttggtttctg cggcgcgcca gccagattcc gatccgacct gcgaaaatcc tacggttaat
    26761 ggagcggcca acgccataga cgacatggct gccgagactg cagagactac aagtaagcgt
    26821 ttccacatag ggttgtcctc gtcggggtaa ttattattgg tgaaaagatg ttcgcgaaaa
    26881 aactatagac aattcgttat gtaacggatt gcgttacatc acacttcaaa gaagtaaata
    26941 aaacgttaat cacaagtttg taatcgcttt catctcacta tgaaaaatgc ggctacggtt
    27001 atggattttc ctgctctgta taccgtctta aaattggcga aaaaggaaaa tgaagacgaa
    27061 aacaagcgaa gacattcggc gcgagttggc tataatactc ggcacttgtt tgccacatat
    27121 ttttaaagga aacagacatg agcttactca acgtccctgc gggtaaagat ctgccggaag
    27181 acatctacgt tgttattgag atcccggcta acgcagatcc gatcaaatac gaaatcgaca
    27241 aagagagcgg cgcactgttc gttgaccgct tcatgtccac cgcgatgttc tatccgtgca
    27301 actacggtta catcaaccac accctgtctc tggacggtga cccggttgac gtactggtcc
    27361 cgactccgta cccgctgcag ccgggttctg tgatccgttg ccgtccggtt ggcgttctga
    27421 aaatgaccga cgaagccggt gaagatgcga aactgattgc tgttccgcac acaaagctga
    27481 gcaaagaata cgatcacatt aaagacgtta acgatctgcc agagctgctg aaagcgcaga
    27541 tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg caagtgggtg aaagttgaag
    27601 gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc ttccttcgag cgcgcaaaga
    27661 ataaataagt tcttctagcg caataaccct gaacgccggg cttcggttag taagggtttt
    27721 tttatgcccg cgataaataa actctctatt ccaccatcat tattctcagc ggttgcaagg
    27781 cttgaacggt aagaacaagc aaacccgacc accattttgc tgttcatagc cacttgctgg
    27841 aagttagccg acctcactca tactcaccgt tcaaagcgtg cccccgtttt acccctgcac
    27901 atttgtgaac gccagcagag ttttttatgc gattgcagat atggaaagcg gctcggagag
    27961 aaaagcaaaa gatggaaaag ttacaaaaaa aaatcgcgag atacatcgca taaaacaacg
    28021 ccacccgaag gtggcgtgct tttattatta cttatccctg tctaaccagt cgccgctttc
    28081 aattagcttt aatccatcga cgggtcgttg ataaacgtac atccatgcac tcccgtacgg
    28141 cgtctgaatc aactggcgcg cgtattcacc gcccctggtg cgcaaggcat caagttcggc
    28201 cagcgtggcg ttgtcaatac gataaacttc accgtgtact gttccgttcc ccggaactgc
    28261 gcctggatag tggcccaggc tatacaactg gtagttatcg atactgaaat cgcccagtaa
    28321 ctgggcattg gtcatccagt gactgttgcc ttgtttgtgg cgtaaactgc cgtagacaaa
    28381 tattcgcatt gctaaaactc gaactgatag agcaaatcca gtgcctggtc tacaccagac
    28441 acggcttcca gatatagctt aggcatcagg cgataacgta acgtgagtgt tgctatagag
    28501 tcaaatatac ccacgccgta tttcacttgc agacctggca atacatagcc gctgaccact
    28561 acctgggagg agtcgcctac tccctgggtg tcgagcgcta aattgcttac gccaaacgtc
    28621 tcgccgattt tacccacaat ctggccactt tgcgcaaccc ccagaccaat cagcatcgag
    28681 gtcattgccg cactgtcgct ctgatcgctc tccagccctt gtccacgtag caaataagac
    28741 aatgcagctt gttgcgacat cgccgggtca gagaagatct ccgctttcgg ttcgtccgcc
    28801 agaccagtga cgcgaactcc ggcgattacg tcgtcttctg tagcatccgg gttacgaata
    28861 gcttcaatat taagatacgg ttgatccggc ggaccagaga acagtaactc acctttacgc
    28921 acaatcagat cctgaccata ggcatggaag cgcccttcag ggatgttgat ctgcccgttc
    28981 aggcccagcc cttgtttgtc ctgaacgacg ttaagatcgc ccgtcagccg cgctttcagg
    29041 ccaaaggcgt caatgcgcac attgttgcca acgtggacaa tcaggttact gttaatcgga
    29101 atcgacgccg ttttcggctc ttccggttgc aggttatcgt taagcatcac cacatcgctg
    29161 gagacgccta ctgcgctttc cggcagatcg tgcaccacga tgcgcgccca cgggacatcc
    29221 acgcgaccat cgagggtaaa caggtttggt gtagcctcga agacaacatc tggcgataca
    29281 tccattcgta ccatcggcgg cacggtgatc cgcactttac tgcctttcgc cgttacccgc
    29341 gcccgccagt tttcaatttg gctccagtcg gcatcaccgt tcaggtagat ttcaccctgc
    29401 tgggtccgta ctgtaccggc aagcgtcgag cgcataccat taaagttgac cgcaagctgg
    29461 ctcggttgca tatcaaacgg cataaagttg ccgtcgatat ccacacccgt aacctgaagc
    29521 tgaccaaaca actgcgggct ttgcacatca ccacccagac gcaagttggc actcaccatc
    29581 cccgctgctt tttccccacg ggtaaagatg gggtttatca tcgcaaggtt gaagttacgg
    29641 atattgacgt tgccaccaag attacggcgg ccttgcggat cggtcacctg cacctgtcca
    29701 tcaaactggc cgttattggt caggcggatg gtccagccca attcggcacg gttgttacgc
    29761 aattccgccg tcagattcag tgtctgaaac gccacgggca gcgccgcatc gttgacggtt
    29821 tgcgttacct gcacgttacg ccccgaaagg gtgatactgc cctgcggcag cccctctttg
    29881 gtggtgtccc aggcaacgtc cgctttaccc gtgaagatac cgctggcctg agtggtttct
    29941 ggcataaatg gtttcagcat ggccaggtcg aagcggttga gattcaccac cgcacgccct
    30001 tcggcacccg catcgatagt ttgcggcacg cacagttccg cattcgggtt aagccagcag
    30061 tgtggcccga tgctgatttt ttgctcctta ttgcggtaat ccagcgcaat atcgcgggtc
    30121 agcgaccacg ggccgaccgg cgtctggaag cgggtattgc taagagttcc cttccagcgt
    30181 tcttctttgc gatcaaaact tcctgccaga ttaagctgcc cggagacagg ctcgccctga
    30241 atccgcaact gtaactcgtg ctgtttttcg ctgcctttgg cattcagggt gacgaggttg
    30301 atatttacat ccggctgcga aatttgctca acgcgtacgt cgagtttccc ggcgatctga
    30361 tcggtggatt taatgtcgcc ttccacgcga acctgcgcca cggaaagttc ctgccagcgc
    30421 aggccgcgcg cggtgatatc tgccagtagt tgtggcgctt ccaccgtgcc gcgtactttc
    30481 accagccctt tcgctgtacc gccaagcccc ggcagcgcgt tatccagccc cggcgcgttg
    30541 atggtggcat caagattgag atcttttacc cccagctcgc ctttcacttc ggcactgttt
    30601 ggccccagtt ccagatgaag ccctgggatc atccactgca tataactgtt gcctttcagc
    30661 gtgccgtcaa cgttcacttt gttctgtttg acgttaccgg tcagcttcag ttctggcacc
    30721 tccatctgcc aggtgccgcc gtacaggcta ccgcgggttt taatcaagcc attgagtttc
    30781 gacggccact ctgggatctc tttggcggtg ttaatgccgt taagcgttag ctcaccgcgc
    30841 caactaatgg cctgctgcca gtcgagcaac gccttgagtt cagttttccc ttccagcgcc
    30901 gcgacggtga gtttgtcgag attgacctgc tgttcattac ctttggcatc aagggtaatg
    30961 gtggctggcg ggatctccag tcccttcact gccgtacgca tagagagcgt gtaatcggtc
    31021 attttgccgg taagtttcag tttcagatca tccgcctgat actgcttctc accagtgaac
    31081 ggccagtaaa tctgtttgct gttcacttcc acgttgagcg gcaatccggc ttccgccagt
    31141 cgcgtctggg cgcgtaaatc catatccacc ggaccggaaa gattaacgcc aatctccagc
    31201 tgttcgcgca gcgcgccgcc cactttcagc ttcacctttt cacctttcaa cggctccacg
    31261 ttcagtgtac tattgagagt gatgtccacc ggccagttgt ctgacagctg cgccgtgccg
    31321 ctggcgttga cgatcccttg actggaatcg atatccaggg cgtccagttt agtattgccg
    31381 tcaatgctgc tcactttcag cagcatggtg ctcacggtga tgtccgtgtc gcccgtcacg
    31441 cgcaactgct cgcccttaaa ctcttcaatg ttcaggttaa gcggcaaatg cacgtcggtc
    31501 atttccggca ataccgggcg agaaaagaga tctttcagcg tttcgccgag cggcttttcc
    31561 tccggctgcg gattttcaat tttcggttcg accacttctt cctgcgccac ttccgccact
    31621 ttcggcagag caatcagcag gcctttcagc gacgtcggtt tcagggtcag ggttttctcc
    31681 tgccagttca ggccggaggt gaagtccatc accgataccg tggtgtcatc aatcttgatg
    31741 ttgacgttgt ccagtgccac ccgtgttagg gtgatgggat acggcgtgga gagatccagc
    31801 ggaccgctat cttcctcttc ttcaacctgt tcagaagggg gcattttttt gctgtcgata
    31861 ttgacctgga tgtctttcag cgccagatca ttaatacaaa cactgctgtt ccacaggcac
    31921 tcaagcccga ccgccagatg cagattgcct gcttttaccg ccacgcctgg ctgctcataa
    31981 cgaacgtcag acaaggtgag atcgcgccag ccgccggtga ccttgccaac atccagtcct
    32041 ggcacccagc gatccgccgc tttaaatacc agatgcaggc cgctggtggt gcccaccaga
    32101 aacgccaccg atcccagcaa cagtaagata acgataacca cgccgaggct gatttttttc
    32161 cataaactca taattctggc cccagaccga tgtaaaactg taacccgtgt tcgtctttat
    32221 ccgcgaccgg tacggcaaaa tcgagtttga ttggcccgac cggcgattcc cagcgtacgc
    32281 cgaccccggt accggtttta aagtcgctgc ggcgaatatc gcttaccgct tcgccactat
    32341 cgacaaacac cgcgccccac cattttccgg tcacgttgta ctggtactcc agcgatccgg
    32401 ttatcaactt cgaggcccct ttcaggtcac cgttggcgta tttcggagcg atagatttgt
    32461 atttgtagcc acgaatactg cggtcgcccc cggcgaaaaa acgcagatcc ggcggtactt
    32521 tgtcgaaatc accggtttca atccagccca gcgtgccgcg tgtcacaaaa cgatggcgat
    32581 cgtacagtgt gcggatccag acgttttgtg cctggaaaac ggagaaatcg acatctgagc
    32641 cccaggcagt gttggagtag tcgatagagt agcgttgcga gtcgccccag gttggcatca
    32701 ggccaccacg agaacgcgtg cggctaatca tcaccccagg ataaaacagc atcgtggtgt
    32761 tggtaatttc accctgggta aagtggtcga gactccagcg caggttaatg gcacgctgcc
    32821 agccgctgga gagatcccag tagcgagaag ccaccagcgt agtggagtca gattcggtat
    32881 cgttcaggtc agtgcgctta aaaccgccct gcaccaaata atattgttcc agtggattct
    32941 tcagcagcgg cattttatag ctgaagtcga ggatctgttc cggcgcggaa atactggtac
    33001 tggtggtcag actgtgaccg tatgagttca tccacggttt tttccacgtc gctttcacgc
    33061 gcggtcccac gtccgtagag taaccgaccc cggtttcgat ggtgttttct gttcgcggcg
    33121 aaaccacgcc cgtcaatggt aatactttcg tttcgcgcgc tttatcaaat tgtggagcca
    33181 ccaccaccga gttaaaccag ccggtagcag aaagtcggcg gttcagttcc gccagatctt
    33241 tcgattcgta ctcatcgccc tctttaaacg gcaccagatt ttgcaggtat tcatcgcgga
    33301 tttgtgatcc ttcaaaggtc acatgcccaa agcggtaacg ttcgccactg ttataatcaa
    33361 tatcccagaa ggctttatgc aggccgagcg caatgcccag ctgcgctttg gtaaattcgc
    33421 tatcgaaata acctttacgc aacgcaatgc tggttaagga ctttttgaaa ttttcataat
    33481 cgccctggtt cagcaccgtg ccaatagccg ggcgagtatc gagcaatttc aaatagtctt
    33541 tatcggtccg cgcgccgccg cgcaatacca catcggtgcc gccaattaac accggcacgc
    33601 ctggcgtgac tttggcgatc aatacctgcc gccctttctt tggcggtgga cggagatcaa
    33661 attcaatggt cggctggtaa taacccagcg ctttcagacc ttcgcggatg gcatcatcga
    33721 cgcgtgcgcg aaagcgacgg tctggcgtca cttcatcact ttcaatcgta gaaagctgcg
    33781 cacgaacgtt cttttccagc tgtcccgata acccctcgac ctgtagacgg acgttcgcgg
    33841 cgacggcaga tccgcttaag cagagtaagc ttacacagca taactgtcgg atatagcgca
    33901 cattttctcc tgaatatcct tttttcctgc ccctggaacg ccgttaaacg gcttaacaaa
    33961 aatccagtaa tatggattaa aaagcagact aaaccccaaa tatttcttat gttttacttt
    34021 agacctattc acggtggtta ttgtgtgcaa atacgcctct tgttacaacc ttaaccccaa
    34081 tgaccgattt tcgggagagc gacaccatga gtttatttga taaaaagcat ctggtttccc
    34141 ccgccgatgc cctgcctgga cgtaacaccc cgatgcccgt agccacgctg catgcggtca
    34201 acggtcactc aatgaccaat gtacctgacg gaatggagat tgccattttt gcgatgggtt
    34261 gtttctgggg tgtggagcgt ctgttctggc agttacccgg cgtttacagc accgccgcag
    34321 gctataccgg aggctatacg ccaaatccga cttatcggga agtgtgctcc ggtgatacgg
    34381 gtcatgccga agcggtacgc attgtttacg atccttccgt catcagctat gagcagttgc
    34441 tacaggtatt ttgggagaat cacgatcccg cccagggaat gcgtcagggc aatgaccacg
    34501 gcacgcagta tcgttcagcg atttatccgc tgaccccaga acaggatgcc gcagctcgcg
    34561 ccagtctgga acgttttcag gcggcgatgc ttgccgccga tgatgatcgt cacatcacca
    34621 cggaaatcgc taacgccaca ccgttttatt atgccgaaga tgaccaccag caatatctgc
    34681 ataaaaaccc gtatggttac tgtggaattg gcggaattgg cgtctgtctg ccaccggaag
    34741 catagcgtta cgggtacaaa tgtagattgt tgataaagtg cgctttattt atgccagatg
    34801 cggcgtaaac gccttaccag gcctacaaaa tcgtccaaat tcaatatatt gcaaggactg
    34861 cgtaggcctg attggcatag cgcatcaggc agtattgcat ttatcagcag tctgaatttt
    34921 aaccctctgg cgactttaca gtaccttacg ctatactagc cactgaaaat gccggttcac
    34981 tttcttcgaa tcggctttca atgtgtattt cacacaaatt aatcaacttc cccttccgag
    35041 gatctggcct gaaaggtcgg ataagatatg ttaaacagta ttttagtcat actctgcttg
    35101 atcgctgtaa gtgcgttctt ctcgatgtcc gagatctcac ttgccgcctc acgcaaaatc
    35161 aaacttaaac tgctggctga tgaaggcaat ataaatgccc aacgcgttct gaatatgcag
    35221 gaaaatcccg gcatgttctt taccgtggtc caaatcggtc tgaacgcagt ggcgattctc
    35281 ggcggtatcg tcggtgatgc ggcattttct ccagcttttc acagcctgtt ctcccgctat
    35341 atgtcggcag agctctctga gcaactgagc tttattctct ctttctcgtt agtgactggc
    35401 atgtttatcc tgtttgcgga tttaaccccg aaacgcatcg gtatgattgc gccagaagct
    35461 gtcgctttgc gtatcatcaa cccgatgcgc ttctgcctgt acgtttgcac cccgctggtg
    35521 tggttcttca acggactggc gaacataatc ttccgtattt tcaaactgcc aatggtacgt
    35581 aaagatgaca tcacttctga tgacatctac gcggtagtgg aagccggtgc gctggcgggc
    35641 gtgttacgta aacaggaaca cgagctgatt gaaaacgtct ttgagctgga atcccgtacc
    35701 gttccgtcgt caatgacacc gcgtgaaaac gtgatttggt ttgatctcca cgaagatgag
    35761 caaagcctga agaataaggt ggcggaacat ccgcactcta agttcctcgt ctgtaatgaa
    35821 gatattgacc acatcatcgg ctatgtcgat tctaaagacc tgctgaaccg tgtgctggct
    35881 aaccaaagcc tggcactgaa cagcggcgta caaattcgca acacgctgat tgtgccggat
    35941 acgttaaccc tttcagaggc gttggaaagt tttaaaaccg caggtgaaga cttcgcggtg
    36001 atcatgaacg agtacgcgct ggtggtgggg atcatcaccc tcaatgacgt gatgaccacg
    36061 ctgatgggcg atctagtcgg tcaggggctg gaagagcaga ttgtcgcccg tgatgagaac
    36121 tcatggctga ttgacggcgg caccccaatt gacgacgtca tgcgcgtgct ggatattgac
    36181 gagttcccgc agtcgggcaa ctacgaaacc atcggcggct ttatgatgtt tatgctgcgt
    36241 aagatcccga aacgcaccga ttcggtgaaa ttcgccggct acaaatttga agtggtggat
    36301 atcgataact accgcatcga ccagctgctg gtgacccgga tcgacagcaa ggccaccgcc
    36361 ctttcgccaa aactgcctga cgctaaagat aaagaagaaa gcgtcgcgta acccccagaa
    36421 acatcaacgg ctcctgaatc aggagccgtc ttattactgc atagcacttt ggttaagcca
    36481 tctctgtttg cagacgcata acctgacggt taacttcgga catcactgac aaatgcagct
    36541 tatccttcac ttttgggata agaatcttac ccttatcaaa ttcaaaagcg ccaacgtcct
    36601 tgatgtataa ccgtccacgg aacaggatct ttacgtactt cgccacctga agtgggttgt
    36661 accgttggaa aattttcatt gttatctcct gctgagtatt acgcctgtgc ggtgccacaa
    36721 tcggcccaac tattatgagg cgcaaatttt aatgcttagt gactatagac tatccgggca
    36781 atgtttccac cgcgtataac tttttttacc taaaggttac aattattcag aattatcttt
    36841 ttaccgaagc gcggtcttca gtataagcat tcatttttca tatgatttgt gcgcttgacc
    36901 gcaaactggc atcacacttg cgggaaattc gataaatagc acatatgatt aaaactcaga
    36961 cccaagtggt cggatcacct gcatatcata agaaggaaac accatgaccc tacgcaagat
    37021 tctggcactc acctgcctgc tgttgccgat gatggcttcc gcacatcagt tcgaaaccgg
    37081 tcagcgagtg ccgccgattg gcattaccga tcgaggcgag ttggtgcttg ataaagatca
    37141 gtttagctac aaaacctgga acagcgcaca gttagtggga aaagtgcgag tactgcaaca
    37201 tattgctggt cgcacctctg caaaagagaa aaacgcgacg ctgattgaag cgattaaatc
    37261 agcgaagtta ccgcacgatc gctaccagac caccaccatt gttaacaccg acgacgcgat
    37321 tccgggttca gggatgtttg tgcgcagcag tctggagagt aataaaaagc tttatccctg
    37381 gtcgcagttt attgtcgata gcaatggcgt cgcgcgcggt gcctggcagc tggatgaaga
    37441 aagttccgct gtagtggtgc tggataagga tggtcgcgtg caatgggcca aagacggggc
    37501 gctcacacag gaagaggtgc agcaagtgat ggacctgctg cataaattaa ttaataaata
    37561 gacactctga atcccggatt caggaacgac tcgcgcggag tgtaatccag cggtttaccc
    37621 tgccagtcat gaacgtgcgc tccggcagct gcagccacag catgaccagc ggcggtatcc
    37681 caaatattcg ttggtccgaa gcgcgggtac agctgcgcct gcccttccgc caccaggcag
    37741 aatttcagcg aagagccgat ggacgtggtc tgatgttcgc caagctgttg cagatactct
    37801 ttcagctccg catctgcatg ggaacggctg ataaccacca gcggcgggcg cgcatcgcgg
    37861 acctgaatct gcttgcgcac accgcactct tctttccagg ccttgccttc tgccgcgctg
    37921 tacattatgt tcattaccgg cgcatacacc acacctaaaa tcggtttgcc atggtcaatg
    37981 agcgcaatgt taacggtgaa ttcgccatta cgtttaataa actctttagt accatccagc
    38041 gggtctacca gccagtaacg ctgccagtgc tgacggactt cccaaccggg aggatcttct
    38101 tcagaaagga ccggaatatc cggtgtcagc gtacgtaaac cgtccatgat aacggtgtga
    38161 gcggcaatat ccgctgccgt taccggagaa ttgtccgctt tgctgacgac gtccatcggt
    38221 ttcgtcccgt cgtagacctg cataatggca tcgcctgcat tccgtgcaag ctggcatact
    38281 tgatctaaca tttctccacc tcgtctctgt gagcggtgtt aacttattgt tttacttata
    38341 ccctatcgtt aatgaatgcg ccaactgtga tagtgtcatc attttcaaag cgtaaaattg
    38401 tggcattctt cactgttcta taagtaaggc gtttattctc ccttttcttt cgtattcccg
    38461 atgataaaag gatgtccctg atgattaagt ttagcgcaac gctcctggcc acgctgattg
    38521 ccgccagtgt gaatgcagcg acggtcgatc tacgtatcat ggaaaccact gatctgcata
    38581 gcaacatgat ggatttcgat tattacaaag acaccgccac ggaaaaattc ggactggtac
    38641 gtacggcaag cctgattaac gatgcccgca atgaagtgaa aaacagcgta ctggtcgata
    38701 acggcgattt gattcagggg agtccgctgg ccgattacat atcggcgaaa ggattaaaag
    38761 caggtgatgt tcatccggtt tataaggcgc tgaatacgct ggattatacg gtcggtacac
    38821 tcggcaatca tgaatttaac tacggtctgg attacctgaa aaatgcgttg gcgggagcga
    38881 aattccctta tgtaaatgcc aacgtcattg acgccagaac caaacagcca atgtttacac
    38941 cgtatttaat taaagatacc gaagtggtcg ataaagacgg aaaaaaacag acgctgaaga
    39001 ttggctatat tggcgtcgtg ccgccgcaaa tcatgggctg ggataaagct aatttatccg
    39061 gaaaagtgac ggtgaatgat attaccgaaa ccgtgcgcaa atacgtgcct gaaatgcgcg
    39121 agaaaggtgc cgatgtcgtt gtcgttctgg cgcattccgg gctatctgcc gatccgtata
    39181 aagtgatggc ggaaaactca gtttattacc tcagtgaaat tcctggcgtt aacgccatta
    39241 tgtttggcca tgctcacgcc gttttcccag gtaaagattt tgctgatatc gaaggggctg
    39301 atatcgcgaa aggcacgctg aatggtgttc cggcggtaat gccgggcatg tggggcgatc
    39361 atcttggggt ggtcgactta caactcagta ataacagcgg taaatggcag gtgacgcagg
    39421 cgaaagcgga agcacggccg atttacgaca tcgccaataa aaaatccctc gcggcggaag
    39481 acagcaagct ggtagaaaca ctcaaagccg atcacgatgc cacacgccag ttcgtcagca
    39541 agccaatcgg taaatccgcc gacaatatgt atagctatct ggcgctggtg caggacgatc
    39601 cgaccgtgca ggtggtgaac aacgcgcaaa aagcgtatgt cgagcattac attcagggcg
    39661 atccggatct ggcaaaactg ccggtgcttt cagctgctgc accgtttaaa gttggtggtc
    39721 gcaaaaatga tccggcaagc tatgtggagg tggaaaaagg ccagttgacc ttccgtaatg
    39781 ccgccgatct ttatctctac cccaatacgc tgattgtggt gaaagccagc ggtaaagagg
    39841 tgaaagagtg gctggagtgc tccgccggac agtttaacca gattgatcct gacaacacga
    39901 aaccacagtc actcatcaac tgggatggtt tccgcaccta taactttgat gtgattgatg
    39961 gtgtgaatta tcagattgat gttacccagc ctgcccgtta tgacggcgag tgccagatgg
    40021 ttaatgccaa tgcggaaagg attaagaacc tgacctttaa tggcaagccg attgatccga
    40081 acgccatgtt cctggttgcc accaataact atcgcgctta cggcggcaaa tttgccggta
    40141 cgggcgacag ccatatcgct tttgcttcac cggatgagaa ccgctcggtg ctggcagcgt
    40201 ggattgctga tgagtcgaaa cgtgcggggg aaattcaccc ggcggcagat aacaactggc
    40261 gtttagcacc gatagctggc gataagaaac tggatatccg tttcgaaacc tctccatcag
    40321 ataaagccgc ggcgtttatt aaagagaaag ggcaatatcc gatgaataaa gtcgcgaccg
    40381 atgatatcgg gtttgcgatt tatcaggtgg atttgagtaa gtaatacact tctttttcgg
    40441 cctataaatc atcaaccgca tccggcactt ttgtcggatg cgatgctggc gcatcttatc
    40501 cgccctacaa gtcatgaacc gtaggccgga taagcgcagc cggcagttat gccgcacgtt
    40561 catcccgcac cgccaacacc tcgggcaaat tcaactcaat ccagtccgcc agtgcagcaa
    40621 ccttttcgct cacctgctcg cccagcggcg tgaggctata ttccacatgc ggcggcacca
    40681 ccggatacgc gatacggtta agaaaaccat cctgttctaa cgcctgtaac gactgcgcaa
    40741 gcatcttttc actcacccca ccgattttgc gccgcaggtc gctaaagcga tgagtacctt
    40801 cgcgtagcgc caccagaatc aacacccccc aacggctggt gacgtgtttc aacacctcgc
    40861 gcgacgggca ctgttccgca aagagattac cctctttcag ttgttgtgac aggctaacct
    40921 gactcatttc atacttacct ttttgtacgt acttactaaa agtaagttta ggtgttagcg
    40981 tatttaaaca caagacaaaa cgatggagac ttcccatgat cgctattact ggtgccactg
    41041 gccaacttgg tcactatgtt attaaatcct tgatgaaaac ggttcctgcc agccaaatag
    41101 tggctatcgt tcgtaatccg gcaaaagccc aggcactgac agcacaaggc attaccgtgc
    41161 gtcaggctgc ctacggcgat gaagccgcac tgacatctgc acttcaggga gtggaaaaac
    41221 tactgctgat ctcttccagc gaagtgggtc aacgtgcccc gcagcatcgt aatgttatta
    41281 atgccgcaaa gacggctggc gtgaaattta tcgcttatac cagtctgctt cacgcggata
    41341 aatctccgct cggcctcgcc gatgagcaca tcgagacgga gaaaatgttg gctgattctg
    41401 gcatcgttta caccctgctg cgcaacggct ggtacaccga aaactatctc gccagcgccc
    41461 cggcagcact ggagcacggc gtatttatcg gtgccgcggg cgatggcaaa attgcctcgg
    41521 caacgcgggc agattatgcg gcagctgcgg cacgcgtgat tagcgaagcc ggtcacgaag
    41581 gcaaggttta cgagctggcg ggcgatagtg cctggacgtt gacacagtta gcggcagagc
    41641 tgaccaaaca gagcggcaaa caggttacct atcaaaatct gagcgaaacc gatttcgctg
    41701 cggcgctgaa aagcgttggc ctgcctgacg gactggcgga tatgctggcg gattctgacg
    41761 ttggcgcatc gaaaggcggt ctgtttgatg acagcaaaac gctgagcaaa ttgattggcc
    41821 gcccaacgac aacgttagcc gaaagcgtaa gccatctttt taatgttaat aaatagttaa
    41881 ttaaagtggc atcctcccgc atcctctctg ataatgacgg gatgccggga gcaatcatgt
    41941 ctgcttcctg aacgtttctt ctgacagacc aatggatgcc agtaatgatt agcggcgtgc
    42001 tgtacgccct gttagcaggg ttgatgtggg ggcttatttt tgtcgggccg ttgatcgtgc
    42061 cggaataccc ggcgatgttg cagtcgatgg ggcgttatct ggcgttaggg ttaattgcgc
    42121 tacccattgc ctggctggga cgcgtgcgtc tgcgtcagtt ggcgcgtcgc gactggctta
    42181 ccgccttgat gctcactatg atgggcaacc tcatctatta cttctgcctt gccagtgcca
    42241 ttcaacgtac tggcgcgcct gtttctacga tgattattgg caccctgccg gtggtcattc
    42301 ctgtctttgc caatctgctt tatagccagc gcgacggcaa actcgcgtgg ggaaaactcg
    42361 ccccggcact gatttgtatt ggcatcggcc tggcgtgtgt gaatattgct gagttaaacc
    42421 acggactccc cgattttgac tgggcacgtt atacctctgg catcgtgcta gcgttagttt
    42481 ccgtggtctg ctgggcatgg tatgccctgc gcaacgcccg ctggctgcgg gaaaatcccg
    42541 acaaacatcc gatgatgtgg gcgacggcgc aggcgctggt cacgctgccg gtttctctca
    42601 tcggctatct cgtcgcctgt tactggctga atatacaaac gccggacttc tccttacctt
    42661 ttggcccccg tccgctggtg tttattagtc tgatggttgc gatagccgtg ctttgctcat
    42721 gggttggcgc actctgctgg aacgtcgcca gccagcgatt accgacagtg attctcgggc
    42781 cgctgatcgt tttcgaaaca ctggcaggtt tgctgtacac ctttttgata cgccagcaaa
    42841 tgccgccgct gatgacgctg agcggtatcg cgctgttagt ggttggcgtg gtcattgcag
    42901 tcagagcaaa accggaaaag cctttaactg aatctgtctc agaaagttga catactggca
    42961 gtgagttaaa taagcctctg ctacgtaagg gttatagcat ttgccttaaa gatgcattta
    43021 aaatacatct tatcttatta agaatgaggt atcagctatg gcttatcgcg accaaccttt
    43081 aggtgaactg gcgctctcta tccctcgcgc ttcggctctg tttcgtaaat atgatatgga
    43141 ttactgctgc ggcggtaagc aaacgctggc gcgtgcggcg gcacgtaaag aactggatgt
    43201 tgaggtcatt gaagctgagc tggcaaagct tgctgaacaa ccgattgaga aagactggcg
    43261 tagcgccccg ctggcagaaa tcatcgacca tatcatcgtg cgctaccacg atcgtcaccg
    43321 cgagcaactg ccggagctga tcctgcaagc gactaaagtc gagcgcgttc acgccgacaa
    43381 accgagcgtg ccaaaagggc tgacaaaata cctgaccatg ctgcatgaag agctttccag
    43441 ccacatgatg aaagaagagc agatcctctt cccgatgatc aaacaaggca tgggcagcca
    43501 ggcaatgggg ccaatcagcg taatggaaag cgagcacgat gaagcgggcg aactgctgga
    43561 agtgatcaaa cacactacca ataacgtcac accgccgcca gaagcctgca ccacctggaa
    43621 agcgatgtat aacggcatta atgaactgat tgatgacctg atggatcaca tcagtctgga
    43681 aaacaatgta ctgttcccac gcgcgctggc gggtgagtaa tctaaaagct ggatggcatt
    43741 gcgccatcca gcatgataat gcggttattt ccgcagttca gcagcccgct tcttaccaat
    43801 aaacaaccag cccagcccca gtgcgataaa ccacagcggg gtgaccaaca gcgcctggcg
    43861 agtgtcatct tccagtgtca gcaacacgac cacgaacaca aagaacgcca tacataccca
    43921 gcacatcagc ttgccgagcg gcatcttgta gatcgacttc tcatgcagat gaggacgctg
    43981 tttgcggtaa accaggtacg agcaaaggat aatcgtccag acgaacataa acagaatcgc
    44041 ggaaacggtt gtaatcatcg tgaacgcgcc aatcacgcta ggattcacat acagcatcac
    44101 cacgccaccg agcaggcaga tgcaggagaa ggtcagccct ttcgctggta ctgcgcgctt
    44161 agaaagttta gcgaacgctt tcggtgccac accttcctgc gccagaccaa acagcatacg
    44221 gctggtagag aagacgccgc tgttagcgga agacgccgca gaggtcagca ccacaaagtt
    44281 gatcacgctt gcggcagcag gcagccctac caacacgaac agttcaacaa acgggctttt
    44341 ctccgggact accgaactcc acggcgtcac ggacataatc acaatcagcg cgaagacgta
    44401 gaacataatg atacggatcg gaatggagtt aatcgcgcgt ggcagtgatt tctccggatc
    44461 tttggtttcc gcagctgttg tacctaccag ctcaatcccc acgaaagcga aaaccgctat
    44521 ctggaatccg gcaaagaagc cacttaaacc tttcgggaac cagccgccgt cattccacaa
    44581 atgcgcgaat gacgcttcca caccggtcgg tgactgaaag tgcatcgcca ccatgaccag
    44641 gccgacgaca atcagcgaca cgatggcgac gattttgatc atcgcaaacc agaactccat
    44701 ctcaccgaac attttcacgg tggcaaggtt gagcgtcaac agcagcacta tcaccgccag
    44761 cgaggcgacc cagtcggaga gatcggggaa ccagaactga gcataagcag tgatcgccac
    44821 cacgtctgcc ataccggtta caacccagca gaaccagtaa gtccagccgg tgaaatatcc
    44881 tgcccacggc ccgagtaaat cggaagcgaa gtcactaaaa gatttgtatt ccagattcga
    44941 aagcagcaat tcccccattg cccgcatcac gaaaaagagc ataaaaccga tgatcatata
    45001 aacgaaaatg atcgacggcc cggcaaggct aatcgttttg ccagacccca taaacaaccc
    45061 cgtaccaatg gcaccgccaa tggcaataag ctgaatatgt cggtttgtga gattgcgccg
    45121 tagcgactgt tcagccggag cctgatcatc ggcaacgact tttacctgat ctaccatgtt
    45181 tttttcttcc tgtacctgtc tgtgttgttc aggctctacg gcctttagtc agtctatgat
    45241 acgacgaatc tgtatcggga ctcatcgata ttaggtaaca atacgcggga tgaatactaa
    45301 gatttagatc taatgttaat tttatgttta aagtgagtgt catatcacgg tgataacgcg
    45361 aaacagctca caaaaataca cgcttacggg gtctttgcaa cacaaaatag cgccgtttgg
    45421 caaactgaag ggtttattgc tgaatgcctg ctcccctctg gttcgagggg aacagaatgc
    45481 gctgcttaga ggatttccag cagttcgact tcaaacacca gggtgctgaa cggagggatg
    45541 gatgcgcctg cgccgcgctc gccatatgcc agttcctgcg ggatagtcag ttcccattta
    45601 gaacctaccg gcatcagagt cagtgcttca atccagccag ggatcacgcc attaaccggg
    45661 aattcagcgg gttcaccacg agcaacggag ctgtcaaaca cggtgccgtc gatcagttta
    45721 ccggtgtaat gaacacgaac gcggtcagta cgtgccggaa ttgcgccttc accctggttg
    45781 atcacgcgga attgcaggcc agattcggtg ctgttcacac cttctttttt ggcattttct
    45841 tcaagatatt tcacaccttc ggcagccatc gcctggaaac gctgacgacg aacggcatcg
    45901 gcgcgttcgt ggatttcacg cagcgcgcga tgcaccacat caaccggaac agccggatgt
    45961 ttgccttcca gcgcatctgc aataccagcc accagcgcct ccggcagcag cccttgcagg
    46021 ccagattcac tcagttgttg gcctacctgt aaaccaatgc cgtagcttgc ttgcgcttcg
    46081 atggtgtcaa aagttggggt ggtcatcgta tttcctttct tagggtataa aagtagcggg
    46141 cagcatatca gccacacttc tttgggtaaa actttgtcgc aggaatgatg acaaatcccg
    46201 gggaaaaaga aacaatagca atatcccgta tgaatataat aaaggcgggt ttaccacgga
    46261 catcatagcg ttagcttatt cataggctat gattgaggaa caagacgcgg agcaggagga
    46321 aaccatgccc gggcgctttg aactaaaacc aaccctggag aaagtctggc acgcgccgga
    46381 caattttcgc ttcatggacc cgctgccgcc gatgcatcgt cgggggatca ttattgccgc
    46441 cattatattg gtggtcggat ttctgcttcc atctgatgat acgcccaacg cgccggtggt
    46501 gacgcgtgaa gcgcagttag atattcagtc gcaatcacaa ccgccgacag aagaacagct
    46561 gcgggcgcaa ttagtcacac cacaaaatga tcctgatcag gtggctccgg tcgctccaga
    46621 acctatacag gaaggccagc cagaagaaca gccgcaaact acccaaacac aaccattcca
    46681 gccagatagc ggaattgata atcaatggcg ctcttaccgt gtcgaacccg gtaaaacgat
    46741 ggcgcagctg ttccgcgacc acggactgcc cgctaccgat gtctatgcaa tggcgcaggt
    46801 agaaggcgcg ggtaagccgc tgagtaattt gcaaaacggg caaatggtga aaatccgcca
    46861 gaacgccagc ggcgtggtga cgggcttaac cattgatacg gggaataatc aacaggtcct
    46921 gttcacccgc cagccggatg gcagttttat tcgtgcgcgg tagatgaagg gtcaaccaaa
    46981 agccagctaa ggattgtctc tttgatgacc ttacgtctgg cttccagtgc ctggggggaa
    47041 accatactga tatgataaac caggccaagc gtatgctggt tgatcaaata atatccgcca
    47101 agcgcagcaa tattaatatt cacctgcaat gggtcgatat ctggcttaaa aatattgtgt
    47161 ttcttccctt catccaataa ggattccatt aactgcaagt gcgcatggtt aatttctaag
    47221 agacgctgtg attttgcata atgcacacct ttactttgat tttcactatg cacaattttt
    47281 aaaaaccagg gattagcaat ataataatcc catgtaaaat caataatttt ctcaatggct
    47341 ttttgtggcg gaagatctga gaaatcaagg gcctttt
//
LOCUS       DAFNBJ010000015        46812 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000015 DAFNBJ010000000
VERSION     DAFNBJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.015"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(345..887)
                     /locus_tag="L2X49_000865"
     CDS             complement(345..887)
                     /locus_tag="L2X49_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN4795998.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(910..1935)
                     /gene="nagZ"
                     /locus_tag="L2X49_000866"
     CDS             complement(910..1935)
                     /gene="nagZ"
                     /locus_tag="L2X49_000866"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBN4795999.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDSRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1946..2770)
                     /gene="thiK"
                     /locus_tag="L2X49_000867"
     CDS             complement(1946..2770)
                     /gene="thiK"
                     /locus_tag="L2X49_000867"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBN4796000.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2751..3392)
                     /gene="lpoB"
                     /locus_tag="L2X49_000868"
     CDS             complement(2751..3392)
                     /gene="lpoB"
                     /locus_tag="L2X49_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBN4796001.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3406..3783)
                     /locus_tag="L2X49_000869"
     CDS             complement(3406..3783)
                     /locus_tag="L2X49_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBN4796002.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPITVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3786..4145)
                     /gene="hinT"
                     /locus_tag="L2X49_000870"
     CDS             complement(3786..4145)
                     /gene="hinT"
                     /locus_tag="L2X49_000870"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBN4796003.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4479..6668
                     /gene="fhuE"
                     /locus_tag="L2X49_000871"
     CDS             4479..6668
                     /gene="fhuE"
                     /locus_tag="L2X49_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HBN4796004.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITSTYQF"
     gene            complement(6728..8161)
                     /gene="ptsG"
                     /locus_tag="L2X49_000872"
     CDS             complement(6728..8161)
                     /gene="ptsG"
                     /locus_tag="L2X49_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBN4796005.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8456..9253)
                     /locus_tag="L2X49_000873"
     CDS             complement(8456..9253)
                     /locus_tag="L2X49_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBN4796006.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKSVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9264..10268)
                     /gene="holB"
                     /locus_tag="L2X49_000874"
     CDS             complement(9264..10268)
                     /gene="holB"
                     /locus_tag="L2X49_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBN4796007.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10265..10906)
                     /locus_tag="L2X49_000875"
     CDS             complement(10265..10906)
                     /locus_tag="L2X49_000875"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBN4796008.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10896..11918)
                     /gene="yceG"
                     /locus_tag="L2X49_000876"
     CDS             complement(10896..11918)
                     /gene="yceG"
                     /locus_tag="L2X49_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBN4796009.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11921..12730)
                     /gene="pabC"
                     /locus_tag="L2X49_000877"
     CDS             complement(11921..12730)
                     /gene="pabC"
                     /locus_tag="L2X49_000877"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBN4796010.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12850..14091)
                     /gene="fabF"
                     /locus_tag="L2X49_000878"
     CDS             complement(12850..14091)
                     /gene="fabF"
                     /locus_tag="L2X49_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBN4796011.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14179..14415)
                     /gene="acpP"
                     /locus_tag="L2X49_000879"
     CDS             complement(14179..14415)
                     /gene="acpP"
                     /locus_tag="L2X49_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBN4796012.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14626..15360)
                     /gene="fabG"
                     /locus_tag="L2X49_000880"
     CDS             complement(14626..15360)
                     /gene="fabG"
                     /locus_tag="L2X49_000880"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBN4796013.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15373..16302)
                     /gene="fabD"
                     /locus_tag="L2X49_000881"
     CDS             complement(15373..16302)
                     /gene="fabD"
                     /locus_tag="L2X49_000881"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBN4796014.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16318..17271)
                     /gene="fabH"
                     /locus_tag="L2X49_000882"
     CDS             complement(16318..17271)
                     /gene="fabH"
                     /locus_tag="L2X49_000882"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBN4796015.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17339..18409)
                     /gene="plsX"
                     /locus_tag="L2X49_000883"
     CDS             complement(17339..18409)
                     /gene="plsX"
                     /locus_tag="L2X49_000883"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBN4796016.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18490..18663)
                     /gene="rpmF"
                     /locus_tag="L2X49_000884"
     CDS             complement(18490..18663)
                     /gene="rpmF"
                     /locus_tag="L2X49_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBN4796017.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18715..19236)
                     /gene="yceD"
                     /locus_tag="L2X49_000885"
     CDS             complement(18715..19236)
                     /gene="yceD"
                     /locus_tag="L2X49_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBN4796018.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19435..20019
                     /gene="yceF"
                     /locus_tag="L2X49_000886"
     CDS             19435..20019
                     /gene="yceF"
                     /locus_tag="L2X49_000886"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBN4796019.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTL
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20131..21090)
                     /gene="rluC"
                     /locus_tag="L2X49_000887"
     CDS             complement(20131..21090)
                     /gene="rluC"
                     /locus_tag="L2X49_000887"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBN4796020.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21663..24848
                     /gene="rne"
                     /locus_tag="L2X49_000888"
     CDS             21663..24848
                     /gene="rne"
                     /locus_tag="L2X49_000888"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBN4796021.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQKVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25045..25998)
                     /gene="flgL"
                     /locus_tag="L2X49_000889"
     CDS             complement(25045..25998)
                     /gene="flgL"
                     /locus_tag="L2X49_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBN4796022.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGFRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(26010..27653)
                     /gene="flgK"
                     /locus_tag="L2X49_000890"
     CDS             complement(26010..27653)
                     /gene="flgK"
                     /locus_tag="L2X49_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBN4796023.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27719..28659)
                     /gene="flgJ"
                     /locus_tag="L2X49_000891"
                     /pseudo
     CDS             complement(27719..28659)
                     /gene="flgJ"
                     /locus_tag="L2X49_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            complement(28659..29756)
                     /gene="flgI"
                     /locus_tag="L2X49_000892"
     CDS             complement(28659..29756)
                     /gene="flgI"
                     /locus_tag="L2X49_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBN4796024.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29768..30466)
                     /gene="flgH"
                     /locus_tag="L2X49_000893"
     CDS             complement(29768..30466)
                     /gene="flgH"
                     /locus_tag="L2X49_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBN4796025.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30519..31301)
                     /gene="flgG"
                     /locus_tag="L2X49_000894"
     CDS             complement(30519..31301)
                     /gene="flgG"
                     /locus_tag="L2X49_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBN4796026.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31473..32228)
                     /gene="flgF"
                     /locus_tag="L2X49_000895"
     CDS             complement(31473..32228)
                     /gene="flgF"
                     /locus_tag="L2X49_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HBN4796027.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32248..33453)
                     /gene="flgE"
                     /locus_tag="L2X49_000896"
     CDS             complement(32248..33453)
                     /gene="flgE"
                     /locus_tag="L2X49_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBN4796028.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33478..34173)
                     /gene="flgD"
                     /locus_tag="L2X49_000897"
     CDS             complement(33478..34173)
                     /gene="flgD"
                     /locus_tag="L2X49_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBN4796029.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34185..34589)
                     /gene="flgC"
                     /locus_tag="L2X49_000898"
     CDS             complement(34185..34589)
                     /gene="flgC"
                     /locus_tag="L2X49_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBN4796030.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIENQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34593..35009)
                     /gene="flgB"
                     /locus_tag="L2X49_000899"
     CDS             complement(34593..35009)
                     /gene="flgB"
                     /locus_tag="L2X49_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBN4796031.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35164..35823
                     /gene="flgA"
                     /locus_tag="L2X49_000900"
     CDS             35164..35823
                     /gene="flgA"
                     /locus_tag="L2X49_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBN4796032.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSAQLAGISDEVR
                     VSIRTAPSLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35899..36192
                     /gene="flgM"
                     /locus_tag="L2X49_000901"
     CDS             35899..36192
                     /gene="flgM"
                     /locus_tag="L2X49_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBN4796033.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36197..36613
                     /gene="flgN"
                     /locus_tag="L2X49_000902"
     CDS             36197..36613
                     /gene="flgN"
                     /locus_tag="L2X49_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBN4796034.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSADISQRWQKITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(36653..38188)
                     /gene="murJ"
                     /locus_tag="L2X49_000903"
     CDS             complement(36653..38188)
                     /gene="murJ"
                     /locus_tag="L2X49_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBN4796035.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38298..39221)
                     /locus_tag="L2X49_000904"
     CDS             complement(38298..39221)
                     /locus_tag="L2X49_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBN4796036.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39223..39870)
                     /locus_tag="L2X49_000905"
     CDS             complement(39223..39870)
                     /locus_tag="L2X49_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBN4796037.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPSVTDMSSAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39881..40465)
                     /gene="rimJ"
                     /locus_tag="L2X49_000906"
     CDS             complement(39881..40465)
                     /gene="rimJ"
                     /locus_tag="L2X49_000906"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBN4796038.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40701..41909
                     /gene="mdtH"
                     /locus_tag="L2X49_000907"
     CDS             40701..41909
                     /gene="mdtH"
                     /locus_tag="L2X49_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBN4796039.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKSFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41973..42620
                     /gene="grxB"
                     /locus_tag="L2X49_000908"
     CDS             41973..42620
                     /gene="grxB"
                     /locus_tag="L2X49_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBN4796040.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42754..43314
                     /locus_tag="L2X49_000909"
     CDS             42754..43314
                     /locus_tag="L2X49_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBN4796041.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43420..44466
                     /gene="pyrC"
                     /locus_tag="L2X49_000910"
     CDS             43420..44466
                     /gene="pyrC"
                     /locus_tag="L2X49_000910"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBN4796042.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44540..44785
                     /gene="dinI"
                     /locus_tag="L2X49_000911"
     CDS             44540..44785
                     /gene="dinI"
                     /locus_tag="L2X49_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBN4796043.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            45075..45329
                     /gene="bssS"
                     /locus_tag="L2X49_000912"
     CDS             45075..45329
                     /gene="bssS"
                     /locus_tag="L2X49_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBN4796044.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            45444..46562
                     /gene="solA"
                     /locus_tag="L2X49_000913"
     CDS             45444..46562
                     /gene="solA"
                     /locus_tag="L2X49_000913"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBN4796045.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIHHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            46610..46723
                     /locus_tag="L2X49_000914"
     CDS             46610..46723
                     /locus_tag="L2X49_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBN4796046.1"
                     /translation="MCRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        11366 a        12585 c        11622 g        11239 t
ORIGIN      
        1 acaacaggtt acagcaatga aaatgttctt cgctattgag aataacatga atggtgcatt
       61 ggtcatacca aaattgatgt taatcaagtt ttgttgctga aaagataggc gactttaccg
      121 ggtgagatca aaaaatagag tttattgttt attatttgag caaattcgga agagtaatat
      181 agggtgaagc gtatatcgag ggcgtttggt cgtcctgata agatgcgcag catcgcatca
      241 ggcattgatg ctcgactgtc ggatgcggcg tggacgcctt atccgactta cagaattcgg
      301 agcgcatcag gtaccggtaa ggttacctga tgcgacgagg gcatttaccc gagggttttg
      361 aacgctttaa tgcgctgtaa atgcggggag atattcttga atttgtgcgt ctgctcttcg
      421 tcccagacaa tctcgtaata atgatgcaac tcttcagatg tccgctggct gttaagtgct
      481 tcatcattac gcgacaaaat aaccagacaa cgatcgcgat tcttctcgcg gaagttggtc
      541 acacacttag tggcaatatc ggcatactct tccgggcgat caatcttgcc ttccatgttc
      601 tcataaggga acaaattagg gttgaagatc acctggcgga tatcgcaaag aaaaccaatc
      661 cgttccgccc agtatccgcc caggccaacg ccgcaaatta gcggacgctc gtcaacgttc
      721 agttgcaaca ttttgtccac ttctttaagc agatgctgca tatcatgttt cggatgccgc
      781 gtactgtagc ttatcaagcg tacatccggg tcaataaact gcaattgtaa gactttctcg
      841 tggttacccg gactgttaga gtcaaaaccg tgtaaataga taatcatcgc ttcctcacat
      901 aagccagggt tagtgacctg ctttctcttc ctgccagcgt tcgtgtaact gattcagacg
      961 ggtgctgatc gctttccagc gagccgagtc catcagttcc tgtcgcgaaa atgaaccttt
     1021 atgatacaaa cgtgtaacac gttctgcctt gatcggtgac agattatcta acacgctgac
     1081 ggccccttta cgattattgc agaccaggat catatcgcaa cccgcatcca gtgatgcctg
     1141 cccgcgttcg gcataactgc ccataatcgc ggcaccttcc atcgataaat cgtcagagaa
     1201 aatcacgccg tcaaaaccca gttcctgacg caaaacggtt ttcagccagt agggagaacc
     1261 gctcgccgga cgcgaatcaa catcactgta gatcacatgc gcaggcataa tggcgtcgag
     1321 tttattttcg cggattaagg aactgaagac cgacatatct ttagcgcgaa tctccgcttg
     1381 tggacgtgga tcgcacggtg tttctttgtg tgagtctgcc gttactgcac cgtgtcctgg
     1441 gaagtgtttc ccggtcgttt tcattccggc ttcatgcata ccatcaataa accggctggc
     1501 aattgccagg gctttttgtg gatcggcatg ataagaacgc tcgccaatcg ccgcgctgat
     1561 atgcccgaca tccagcactg gcgcaaagct gatatcaata tccatagcga tcatttcgct
     1621 ggccatcagc caacccgcct cttgcgccag tttgccgccc tcttccattc ctgacagcgc
     1681 agcgaatgat tgcgccgctg gcaagcgggt aaaaccttca cgaaaacgct gcacgcgtcc
     1741 accttcctga tctaccgcca ccaccagatg attgcgcgaa gctgcgcgga tctggcgcac
     1801 cagttcacgt aactgggcag gatcatgata gttacgcgta aagagaatca gccctcccac
     1861 cagcggatgc gcaagtattt cacgctcttc cgcgtccagt tcgtaacctt tgacatccaa
     1921 cattactgga cccacactgc tctccttatt gttttattaa tagctgccgc caggtgtcat
     1981 cggccagcct gataaattgt tgatcgccgg tttgtcgcca gcggtactca aaccaccctg
     2041 ctttgagcat cagcagccag ggaaaccatc gcctgacctg acgccataat tgcgccggat
     2101 aaatcttcgc gcgagtggca tagtcattga ccaattgccg gtgctgttca gtattttcca
     2161 cccacaccgc cgccagttcc agcgcgatat caccatctcc tgcatactcc cagtcgatga
     2221 gttttaaccc tgacgcgcta tgcactaaat ttccggcgtg gacatccata tgcaatggac
     2281 ttaagcgtaa aggccgtggt tcccgcgctt tgcgcagacg ttttaacatt cgcagccaac
     2341 ccactgtccg ccgcgccgga tcgctttgct gccagtacag ttccagtaac ggcaacagcg
     2401 ttattcgcca gccaaaacgt ggttgttgat gtagataata cagcaagcct gccagttcgt
     2461 tggtatccgg caaatacgtt tttaccgcgc cgggcagata gtcgactacc atccagtcac
     2521 ggagatataa atgcggcttc ggtgcaatgc atgcgggtag ttgtgataaa gcccgatact
     2581 ggcgcaagaa cgcggactgc ggcgcatcag gatcgtgcgg ctgacgcaca acaaaacgct
     2641 ggccctggtg ttcaatgaga aaactcccgc cactaagccc actattaaac gtcgtgacgg
     2701 gatgaaactg cgggaaaaag cgcgacagca attcgtcgcg cgtgatggga ttattgctgc
     2761 gaaacggcac ctttacctga ccagataatt tcgcccgtct gcaccagcat cagctgcatt
     2821 tgtagggtcg gagcgttaac gttgccagag gcgctggagt atagcacgta atgagcgccg
     2881 acattgcggg caatgcctat ggctttacta cgggtaccta aactgtcctg cggcgacaaa
     2941 cctaactgtt gcttagccat cgacagctgc tgggcggaaa ccagggtaaa tttaccgtta
     3001 ttagccagcg catttcgcag cgtttcggtc gcttctgcgg cattcagcga accgttagta
     3061 cggttgttaa cgctatcaac cagcaggacg ctacccgcag tcaccccgtc agccccaagc
     3121 atcttactga ccatcggctg cattgcgcca ttccagtcat aatggcgaat atgcggcgca
     3181 ggcggtgcag tttgatcttc gtgctcaatt gggcctggct gctgcgggat cgtcggcacc
     3241 gagggcactg tggggacagg ctgttgtggc tcggctggtt gttccggcgc tggtttcact
     3301 tcttctaccg gtgcaggttc acgttgcccc acacacccgg cgagaaacat cgccagcgcg
     3361 gtaatcaagg cgtagcgact catttttgtc atcaagattc accccttaca aatatagata
     3421 aagtctgact ttgtgcgccc ccagaaaatt ggcgctgccg tacagcgtta ccgccgaatg
     3481 tgcgggaatg gtaacgctgc gtggcctttc cagaggatgc atctccagcc ctctggcgtc
     3541 ataccagtaa aaacgataat gaacggtaat gggttcttgc ctttcgttat aaagcgttga
     3601 ggatgctgaa ggttgaatat cagacgtcga aaggacgggc ttttctgcac tgatgcccgc
     3661 agccaataaa gatgactcca tcaccagcga ttgctcatca ttcaccggaa tttccggatg
     3721 tgaacgacag cccaccagca gtaacaacgc cagagacacc agcccaaagc atccttttct
     3781 catcgttaca gacctttatg cgccagcatt ggtcccagcg gacggccacc caacaagtgc
     3841 atatggatgt ggtaaacctc ttgtccgcca tggcggttgg tattcatgat cagacgatag
     3901 ccatcttcgg caataccttc ttgctcagca atttttgccg ctacggtgat catgcgtccc
     3961 agcgcctgct catgctcagc tgacacgtcg ttcacagtcg gaatgaggat attcggaatg
     4021 atcagaatat gcgttggcgc ttgcggcgaa atatcgcgaa acgccgttac cagatcatcc
     4081 tggtagacga tatcggaggg gatctcacga cgaataattt tgctgaatat agtttcttct
     4141 gccacgacat tttccttttt caaatagcca tgcgttgcgc catgctacgc cgcgccacac
     4201 tgcgagtata gagtatggtc gagttaaacc tgctctttca accttaactc acgatttctt
     4261 aagcaaaaaa tcgttttgct gccgtatata tcgccattat tcccatttct gcctgcgaat
     4321 gcgctgaaaa gacattctct gtttggttta cacgacaaat atgaatacgt atatttctca
     4381 tttgcattta caagcaaaat tattcgcaca ctaaatataa ccgctcatca ccctcctccg
     4441 gcatgagcct gacgacaaca taaaccaaga gatttcaaat gctttcaaca caatttaaca
     4501 gggataatca atatcaagcc atcaccaaac cctcactact tgccggttgc atagcattgg
     4561 cactattacc ttctgccgct tttgctgcac cagccactga agaaacggtg attgttgagg
     4621 gttcagccac agctccagat gatggcgaaa atgattacag cgtaacgtct acctctgcgg
     4681 gtaccaaaat gcagatgact caacgtgata ttcctcagtc ggtcactatt gttagccagc
     4741 agcggatgga agatcagcag ttacaaacgc tgggcgaagt gatggaaaac acgctgggga
     4801 tcagcaaaag tcaggcggat tccgatcgtg ctctttatta ttcccgcgga ttccagatcg
     4861 ataactatat ggttgatggt atccccacct attttgaatc gcgctggaat ctgggcgacg
     4921 cactttctga tatggcactg tttgaacgcg tagaagtagt gcgtggcgcg acaggactca
     4981 tgaccgggac gggtaatcca tctgcggcaa ttaatatggt tcgaaaacac gcgaccagtc
     5041 gtgaatttaa aggcgatgtc tcggcggaat acggtagctg gaacaaagaa cggtatgtgg
     5101 cggatttaca aagcccactc accgaagacg gtaaaatccg cgcgcgaatt gtcggcggct
     5161 accagaataa cgactcatgg ctggaccgct acaacagtga aaagaccttc ttctcgggca
     5221 ttgtcgatgc tgatttaggc gatcttacga cgctttcagc cggttacgaa tatcagcgca
     5281 ttgatgttaa tagccctacc tggggcggtt taccgcgctg gaatactgat ggcagcagca
     5341 acagttacga tcgtgcacgc agtaccgcac ctgactgggc gtacaacgat aaagagatca
     5401 acaaggtctt tatgaccctg aagcagcggt ttgctgatac ctggcaagcg acactgaatg
     5461 ccacccactc tgaagtcgaa tttgacagca aaatgatgta tgtcgatgcc tatgtaaaca
     5521 aagcggatgg tatgctggtt gggccataca gtaattatgg acctggcttt gattatgtcg
     5581 gcggcaccgg ttggaacagt ggcaaacgta aagttgatgc gctggatttg ttcgctgacg
     5641 gtatttatga attgtttggt cgtcagcaca atctaatgtt tggtggcagt tacagcaaac
     5701 aaaacaatcg ttacttcagt tcatgggcca acatcttccc ggatgaaatt ggcagtttct
     5761 acaactttaa tggcaatttc ccacaaaccg actggtcacc acagagcctg gcacaggacg
     5821 ataccacaca tatgaaatcg ttatatgctg ccactcgagt cacccttgcc gatccgctgc
     5881 atctgatcct cggcgcacgt tataccaact ggcgggttga tacgctgact tacagcatgg
     5941 agaaaaacca caccacgcct tacgctggtc tggtgtttga catcaatgac aactggtcga
     6001 cctacgccag ctatacctct attttccagc cgcaaaatga tcgtgacagt tcaggcaaat
     6061 atctggctcc aatcaccggt aacaactacg agctgggtct gaaatcggac tggatgaata
     6121 gccgtctgac caccacgtta gccatcttcc gtattgagca ggataatgtc gctcagtcca
     6181 ccggtacacc tatccccggc agcaacggcg aaaccgccta taaagcggtg gatgggacag
     6241 tcagtaaagg ggtggaattt gaactcaacg gcgcaattac cgacaactgg cagctgacat
     6301 ttggcgcaac gcgctatatt gcagaggata acgaaggaaa cgccgttaat cctaatctgc
     6361 cacgcaccac ggttaaaatg ttcaccagct atcggttgcc tgtcatgcca gagttgacag
     6421 tcggcggtgg tgttaactgg caaaatcgcg tgtataccga caccgtgaca ccgtatggca
     6481 ccttccgcgc cgagcaaggt agctacgcgc tggtggatct cttcacccgc taccaggtga
     6541 cgaaaaactt ctcgttacag gggaacgtca ataacctgtt cgacaaaacc tacgatacca
     6601 acgtggaagg ttctatcgtc tacggcacac cgcgtaattt cagcattacc agcacgtatc
     6661 aattctgaat aacacctgta aaaaaggcag ccatctggct gccttagtct ccccaacgtc
     6721 ttacggatta gtggttacgg atgtactcat ccatctcggt tttcaggtta tcggatttag
     6781 taccgaaaat cgcctgaaca ccagaaccag caaccactac gcccgctgcg cccagtttct
     6841 tcaggccggc ctgatccact ttagacacat cagcaacgct gacgcgcaga cgggtaatac
     6901 atgcgtcgag gttagtaatg ttttctttac caccaaatgc agcaaccaga gccggtgcca
     6961 tttcgctggt acctgtcgct tttgcatctt cagtcgcgtc ttcacgaccc ggcgttttca
     7021 gatccagtgc tttaatcagc acgcggaaga tggtgtagta aacaatcgca taaccgatac
     7081 cgacgatcgg gaacagccac agtttgctgc tgttaccaga cagaacgatg aagtcgatca
     7141 gaccgtgcga gaacgacgta ccgtcacgca tccccagaag aatacagatt gggaatgcca
     7201 ggcctgccag aatcgcgtgg atgatgtaca ggatcggcgc aacgaacatg aaggagaact
     7261 cgatcggctc ggtgataccg gtcaggaacg aggtcagcgc cgcggaaatc ataataccgc
     7321 ccactttcgc gcggttttct ggtttagcag agtgccagat agcaattgcg gcagctggca
     7381 gaccgtacat tttgaacagg aagccaccag acagtttacc cgcagtcggg tcacccgcca
     7441 tataacgcgg aatgtcgccg tggaaaacct gacctgctgc gttggtgtat tcaccaatct
     7501 gcatctggaa aggtacgttc cagatgtggt gcagaccaaa cggtaccagg caacgttcga
     7561 tgaaaccgta aatgccaaac gcaactaccg ggttctggta agcagcccac tgagagaagg
     7621 tctggattgc agaaccaatc ggcggccaaa taaaggacag cacaacgcca gtaaagatgg
     7681 cagccaggcc agaaatgatc ggcacaaagc gtttaccggc aaagaagcca agatactcag
     7741 gcagcttaat acggtagaaa cggttaaaca tgtacgctgc gatcgcaccg gagataatcc
     7801 ctccgagtac gccagtatcc gccaggtgtt tagaggcgat ttcttcagca ggtaaatgca
     7861 gtaccagtgg cgcaaccacg gccatggttt taaccatgat gccataggca acaactgcgg
     7921 ccagcgcgga tacgccatcg ttattggtaa agccgagggc gacaccgatc gcaaaaatca
     7981 gtggcatgtt tgcaaagacg gaaccgcctg cttctgccat aacatgcgat acaacggcgg
     8041 gcagccagct gaaattcgcg gaaccgacgc ccagcagaat acctgcgata ggcagtacgg
     8101 ataccggcag catcagcgat ttaccgacct tttgcaggtt agcaaatgca ttcttaaaca
     8161 taattgagag tgctcctgag tatgggtgct ttttttacgt tctcacgcgt ggcaaggggg
     8221 gagagcctcg ccgtgtacag ggcatctaag cgccctttat ttattacaca gagtaaaata
     8281 attcagtgcc aatttgtttg acggctatca cgtttcaact tcagatcatt ggagaagttg
     8341 cacattttga caaaacatat gtcacaatat ttcaaacgcg gttattcacc gcccaatttt
     8401 cggcggtgaa ctttactcgt tttagccatt aattacgagc tttaaaaaaa ctcattcaac
     8461 ggatggattg aaggcgggaa gcgtcgatgt gaaacagacg ggcgaagtta tcggtggtta
     8521 cctgcgccag ttcttcaacg gcaacacctt tcaacacagc catgtattct gcaacgtcac
     8581 gaaccatcgc aggttgattc tcttttcctc gatgcggtac cggcgcaagg taaggtgagt
     8641 cagtttccac cagtaatcga tccaggggga cataacgcgc agcatcgcgc agttgctccg
     8701 cattacggaa ggtcacaatg ccggaaaagg agatgtaaaa tccgagatcc agtaatttac
     8761 ccgccgtttc tctgtcctct gtaaaacagt gtagtacgcc accgcaatcc gtcacttttt
     8821 cttcgcgcag aattgccagc gtatcggcgc gggcgtcacg ggtatggacg ataaccgact
     8881 tgttcagttc acgaccaatc tggatatgat ggatgaagga ctcttgctga cgtactttag
     8941 tttccggcgt gtaataataa tccagcccag tttcacccag cgcaaccaca ccctcttctg
     9001 ccgccagacg gcgtaaatct tctacatcgt agggatcatt ctggttgagt ggatgcacgc
     9061 cacaagaaaa tacgacgttg tcacgttcgc ctaccagatc ccgcatgtgt aaataacccg
     9121 gtaacgttgt ggcgactgcc agacaaaatt tcacatcgcg tgcggcggct ttcgccagaa
     9181 cgtcatccac gtccttatgc aaagattcat aatccagacc atcgagatgg cagtgtgagt
     9241 cgactaaaaa catgatgtct ctcttaaaga tgaggaaccg gtagcacaac gcccggttgc
     9301 aggtaatgct caatacgcag taaaagatcg gtgatgagaa gctcgcggtt gatgcctgta
     9361 acagacatta actgttcacg aatgtggcaa acatccccca gtatagcctg caggcgcgag
     9421 ggagaaagat ggtttgccag ttcggcgacc aggcccggca catcaacatt ggtcacctgc
     9481 gcagcaccat gatggcgttt tagcgcatcc atcagcaacg ttgccagcca gtgtaaacgc
     9541 gccggagctt gttcatgatt aagggccgct agcagcgaat accagtcgcc cgatggcacg
     9601 ctatatgcca acgcctgaca caatgtttca cgagcctgcc agttatctcc ctgaaacaac
     9661 gccagtgccg cgccaggcga accggcgctt aagcgcaatg cggcaagtaa tgcatcctgt
     9721 gacattgtca cttcgcgtga aagccaggtc acggcgtact gttccggcgg cggcgcaagg
     9781 taatgtaacc gacaacgact acgtaatgtt gccagtaaac gttcaggctc gcgggtagcc
     9841 aggaaaaacc aggtttctgc tggtggctct tcaagcgttt tcagcaatgc gttagccgcg
     9901 gcgtcggtta gtaaggcagc atcggttacc cagacaactt tcgcaccacc taggcgtgcg
     9961 tgctcattca gcttttcggt gacctcacgt accgcatcaa tgcccagcat atttttccct
    10021 ttttcgggag ccagggtgta gtaatcggga tgcgtgccag cctgcatcaa ctgacatcca
    10081 cgacagtgac cgcaactttt gtggccctgc ggttgttggc agagtaaata acggctcagg
    10141 gcgtagatta aagcatcatc gcccatgccc ggtaacgcct gaatgagtag cgcatggtga
    10201 cctcttccgg cctgatagct ggctaccagt ttttcgaaat caggtcgtaa ccatggatac
    10261 catctcatgc gtccaactcc ttaacccagt gggtcacggt agtgcggatt gcatccatca
    10321 cggcctccag cggctgggtg gcatcaatgg tatgaatact tttatcttgt gcagccagtt
    10381 ccagatagcg ggcgcgggtg cgattaaaga aatcgaaaga ctcttgctca atacgatcca
    10441 gctcgccgcg cgcacgcgcg cgttttaagc caacttccgg ggtaacatcg agatagagcg
    10501 ttaagtcggg gcgaaaatcc ccgagaacag catcacgcag tgttgccagc atatgttggt
    10561 caataccacg tcctccgccc tgatacgcct gagtggagag atcgtggcga tcgccaatca
    10621 cccaggtgcc gttagccaga gctggtttga tgaccgtttc taccagttga acgcgcgcgg
    10681 cataaaacat cagaacttcg gctttatcgg taatgacttc atcgcctacc gatttgatat
    10741 ccagcaccag gcttcttaac ttttcggcaa gttgcgtacc gccaggttcc cgagtgaaaa
    10801 ccatgtcgcg gatacccagt tgctcaagcg tctcaaccac cacattacgc gcggtagttt
    10861 tgcctgcgcc ttccagcccc tcaatgacga tatacttact gcgcattttt ttccttaagc
    10921 actttcagat aatcctgcac agacttgtta tggctggcaa gattggtatt aaacgtgtga
    10981 ccacctttac catcggccac aaaatagaga tacggcgttt ttgccggatg cgcagcagcc
    11041 ttcagcgaat ccgcccccgg cgtagctatc gcacccggcg gcaaaccggt aatggtatag
    11101 gtgttatacg ctgtcggcgt ttccaggtct gcacgagaaa gtttgccatt ataacgctct
    11161 cccatcccgt aaatcacggt tgggtcggtc tgcaggcgca taccaatgcg taaacggttg
    11221 ataaataccg atgcaacctg atcgcgttca ctggcaacgg cggtttcttt ttcgataatt
    11281 gatgccatcg tcaccagctg atttttatct ttataaggca gaccgtccgc acgcccttcc
    11341 caggcgctat cgaccgcttt caccattttc ttgtgcgctc gcttgagtaa cgcgacatcg
    11401 gtggtattgg cggtatacat ccaggtgtct ggccagaacc aaccttcaat ccactccggg
    11461 ttttccagtt caagtgcctg cgctacggtg gcgtacttat cgtcgctcag cgtatgcttg
    11521 atatacgggg cctcacgcaa ttgcttgagg taatcgctca gacgcatccc ttctaccagt
    11581 cgcagaggga actgtgcttc tttaccgctt tccagcaact tcagcatctc gcgcacggtc
    11641 atctgcggtg taaagcggta agtcccggct ttaaagtgag aaagatccgg ttcgatacgc
    11701 agcagccatt gaaaaacccg tggacgattg atgatcttat cggcataaag ctgttcaccg
    11761 agcgccagac gtccggtccc tggcttcagg gtaaatatcg tctcttcttt gataagcaat
    11821 ttgctgtcgg caagatggcg aaccttccag acgcccacac cagcggcgat acccagtacc
    11881 accagcaata acaagataat caataacact tttttcatga ctaattcggg cgctcacaaa
    11941 gtggggctaa atattcatat aacgttgctg acgaaaagga gacatcgcca caggcacgta
    12001 cgggcatcac tggcattaac gcattacaaa taaccatctc atctgcctgc aacgcctctt
    12061 ccagagaggc ttgcacttcg acaagctgat aagaggattg tgccagcaaa cggatacaga
    12121 attgtcgcat aatgccgtta acacctgcct gatccagtcg cggcgtataa actacattgc
    12181 ccttccgcca gaacaaatta gccgcacagc attccgtaac ccacccttcg ctgtcaagga
    12241 ccagcgcctc atcagcgttt gtctgctcaa gatgagagcg gatcaatact tgctcaagcc
    12301 gattaagatg tttaatacct gcaagatgag gattgcgccc cagccgcacc gggcttagcg
    12361 ccaacgtcat cccttcgtta cgcaaacggt cgtaatgtgc aggataaacc gtaacggaga
    12421 gaatccgcgt tgccggtcca ctgttcaatg tgctgtaccc tcgcccgcca ctaccgcgac
    12481 tgatcacgac tttcagcaca ccattttgct gttctgctgc cagcgttttc atctcttgtt
    12541 caagctgagg ccagaagtca cagaaaatca tcaaccgctg acaagcatcc tgtagtcgct
    12601 ggatatgcgc cgataacaaa ctgactttac cgtcgataac tctggcggtg gtaaaacaac
    12661 catcaccaaa ctgcgttgcc cgatcgctta ctgccagcga ttcctgctta taaccgttaa
    12721 ttaagaacat accggctcct tatcagagaa ttgttagtgt ggcagcatgt tcactacgga
    12781 acaagtcgga ataaaagcta agaaaaaagg cccgcaagcg gaccttttat atgggtggaa
    12841 aatgacaact tagatctttt taaagatcaa agaaccatta gtgccaccga agccgaagga
    12901 gttacacagc gtgtattcca ttccgctgac ctgacgtgct tcatgcggta cgaaatccag
    12961 atcgcaacct tcatccgggt tatccaggtt gatggttggc ggaacagcct gatcgcgcag
    13021 cgccaggatg gagtagatag attctactgc acccgccgca cctaacaggt gaccggtcat
    13081 agatttcgtg gagcttacca acacacggct tgcagcttca ccgaagatgg ttttcaccgc
    13141 ctgcgcttca gctttatcgc cagccggcgt agaagtaccg tgcgcgttaa cgtagccaat
    13201 ctgactcgct tcaatgcctg catcacgcag agcatttgcc attgccagag ctgcgcctgc
    13261 gccattttct ggcggtgacg tcatatgata agcatcgctg ctcataccaa agccgacgag
    13321 ttcagcgtaa attttcgcac cgcgtttttt cgcgtgttcg tactcttcta gtaccagcat
    13381 accggcacca tcgcccagta cgaagccatc acgctcttta tcccacgggc ggctcgccgc
    13441 ttgcgggtta tcattgcggg tagataatgc acgtgccgcg ccaaaaccac caacgcccag
    13501 cggcgtactg gctttctctg cgccacctgc aaccatcacg tcagcatcgc catacgcgat
    13561 aatacgcgca gcatgaccaa tgttgtgcac gccggaagta caggcggtcg cgatagagat
    13621 gcttgggcca cgcaggccat acatgatagt cagatgacct gccaccatgt tcacaatcgt
    13681 tgacggaacg aagaatgggc tgatcttacg tggaccaccg ttcatcagag atgtgtggtt
    13741 ttcttcgatc agtccgaggc cgccaatccc ggagccaatt gcggcaccaa tgcgggttgc
    13801 gttctcttcc gttatttcaa ggccagaatc ctgcatggcc tgaacgccag cgacaattcc
    13861 atattgaatg aaggcatcca tcttgcgctg ttctttgcgc gagataatgt cctcacagtt
    13921 aaaatccttt actaagccag caaatttcgt tgcataggcg ctagtatcga aatggtcgat
    13981 taggctgatg ccactctgac cggcaagcag agctttccag gtagactcta cggtattgcc
    14041 gacaggagac aacatgccca gtccggtcac aactacacga cgcttagaca cgtttgtcct
    14101 ccagggaggg aaaaaatgat tctagtggga caaaaagata aaactcaggc ggtcgaacga
    14161 ccgcctggag atgttcactt acgcctggtg gccgttgatg taatcaatgg cagcctgaac
    14221 ggtggtgatt ttctcagctt cttcgtccgg aatctcagta tcaaactctt cttccagagc
    14281 cattaccagc tcaacggtgt caagagaatc cgcgcccagg tcttcaacga aagaagcatt
    14341 gttggtaact tcttcctgct taacgcccag ctgttcgccg ataattttct taacgcgttc
    14401 ttcgatagtg ctcatactct taaatttcct atcaaaactc gctttcgcga tggttttcgt
    14461 agtgtataaa atgttgaaaa atttgcaact aaatcccggc aggtcttacc acgattttac
    14521 gttattttga ggccttaccc cccaataacg caaatcattt tgcactaatt attactcata
    14581 accacgcaga ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga ccatgtacat
    14641 cccgccgttc acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg atgccaggaa
    14701 tgcaaccgcg ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa cctgcgccag
    14761 gatacccgca cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa taaagcccgg
    14821 agcaacaacg tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg atttactgaa
    14881 gccgatcaag cccgctttcg ccgcagcgta gttggcctga ccgccatttc ccatggtacc
    14941 aaccacagaa ccgatagtga taatacgacc atgacgcttt ttcatcatag cgcgcattac
    15001 cgcttttgac agacggaaaa cagatgaaag gttggtttcg ataatatcgt tccactcttc
    15061 atctttcatt cgcattaaca ggttatcacg agtgataccg gcattattga ccaggatatc
    15121 cacttcacca aattctgcgc gaattttttc cagaacagat tcgatagatg ccgggtcggt
    15181 cacattcaac atcagacctt tgccgttagc acctaaataa tcactgatcg cctgagcgcc
    15241 attttcactg gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg tttcagcaat
    15301 tgcgcggcca attccgcggc ttgcaccggt taccagtgcg atttttcctt caaaattcat
    15361 gattttcctc ttttaaagct cgagcgccgc tgccatcgct gaaggttcgt tcagcgccga
    15421 ggcggtcagg gtgtcgacaa tgcgtttcgt caggccagtt agcactttgc ccgggccgac
    15481 ttcatagaga tgttctacgc cttgcgctgc catgtactca acagacttcg tccactgaac
    15541 cgggttatac aactgacgta ccagtgcgtc acgaatggca tcaccattgg tttcgcattt
    15601 cacatcaacg ttattcacaa caggaactgt tggtgcgtta aaggtgattt tcgctaattc
    15661 tactgccagt ttgtcggctg ctggtttcat cagcgcacag tgagacggta cgctcactgg
    15721 taacggcagc gcgcgttttg cgcccgcagc tttacaggca gcgccagcac gctcaaccgc
    15781 ttctttatga ccggcaataa ccacctgtcc cggagagtta aagtttaccg gagaaacgac
    15841 ctgaccttct gcagcttctt cacacgcttt cgcaatagac gcatcatcca gaccgatgat
    15901 tgccgccata gcgccagtgc cttccggtac ggcttcttgc atgaacttac cgcgcatctc
    15961 aaccagacgc accgcatcag cgaaatcaat cacgccagcg caaaccagcg cggagtattc
    16021 ccccaggctg tgaccggcca tcattgccgg tgctttaccg ccctgctgct gccatacgcg
    16081 atacagcgca acagatgcag tcaacagcgc tggctgggtt tgccaggttt tattcagttc
    16141 ttcagctggc ccctgctggg tcagcgccca caggtcgtag cccagcgccg cagaagcttc
    16201 agcaaacgtt tcttcgacaa ttggatagct cgccgccata tcagccagca ttccaacggt
    16261 ttgagaaccc tgtccaggga acacaaatgc aaattgcgtc atgttttaat ccttatccta
    16321 gaaacgaacc agcgcggagc cccaggtgaa tccaccgcca aaggcttcaa gcagaaccaa
    16381 ctgccccggc ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg ggacagaggc
    16441 cgcagaggta ttaccgtggc gatccagcgt caccacgaca ttgtccatag acataccgag
    16501 ttttttcgcc gttgcactga taatacgcag gttagcctga tgcggaacca gccagtccag
    16561 ttgagaacgg tcaagattat ttgccgccag cgtctcatca acgatgtgcg ccagttccgt
    16621 taccgcaacc ttgaagactt cgttgcccgc catcgtcaga tgaattgaat tctctggatt
    16681 cacacggtca gcattaggca gcgtcagcaa ctcaccatag ctaccgtcgg catgcagatg
    16741 ggtggagatg attcccggct cttcagaggc agccagcacc gcagcgcccg cgccatcgcc
    16801 aaaaataata atagtcccac gatcggttgg atcgcaggtg cgcgccagta catcggaacc
    16861 gacgaccaga gcatacttca ccgccccaga tttcacgtat tgatcggcta cgcttaatgc
    16921 ataggtgaaa cctgcgcagg ctgctgcaac gtcaaatgcc gggcaacctt taatgcccag
    16981 catgctttga atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag tcgttgccac
    17041 aacgatcagg ccaatctggt ctttctcaat gcccgccatc tcaattgcgc gtgtcgccgc
    17101 ttcaaagccc atggttgaaa cggtttcgtt tggcgcggca atgtggcgtt cgcggatacc
    17161 ggtacgagtg acaatccact cgtcagaggt gtccaccatt ttttccaaat cggcgtttgt
    17221 ccgcacttgt tcgggcagat agctgccagt accaataatc ttcgtataca tgtacgctca
    17281 gtcacttttc ggttatatac cgtcacttgc aaactgcgag ttcgctggca gcgtcctgct
    17341 accgcagagt tccgcttttg ccaccgtcca gcagctcaaa accagctggg tatacagatt
    17401 ccaggcgagc ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact gcctgttcaa
    17461 tcgcgaccgc aaaagctcgc tgattggctg caccatgact ttttatcacc gtgccgcgca
    17521 atcctaacag acaggcgccg ttatactggt cggggttgag gtgactgaat cgcctcgtca
    17581 ggctcttttg tagccaacgc tttaataaca gtagccacca cgaccgtttt ttcccttcac
    17641 cctgagattt cagcagagaa aggaacatcc tgacaacacc ctccatcgtc tttaatgtga
    17701 catttcctgt aaagccgtca caaaccagca catctgtctt gccagttaac aactcattgg
    17761 cttcaagata gccgatataa ttgatagaag ggattgtttt aagcaccgct gaggcatccc
    17821 gaatactgtc gagacccttt acttcttctt caccaatatt gagcaacgcc acgcgaggat
    17881 tgggaatttc caccacctct tcagccagaa ctgagcccat aatggcaaat tgcaccaaca
    17941 ttgtactgtc acaatcgacg ttggccccta agtcaaggac caccgttttg cccttttgct
    18001 gatgtggtaa taccgtcacc agcgccggac gctcaatccc ctccaggggc ttgagtaata
    18061 attttgccag ccccatcagt gccccggtat taccggcact gacacaggct tgcgctcgac
    18121 cttctttcac cagctccagg gccacgcgca ttgaactccc acgactggcg cggatagctt
    18181 gcgaaggccg ggcatcactg gcgataactg actgcgcagg aataatctgc agacgcgaac
    18241 gttgttcaaa gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg ccgactaaaa
    18301 gaagagtgag ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact gtcacggaag
    18361 ggccaaaatc ccctcccatg acatctaacg ccagggttag acgtgtcaag gtatcgtcgc
    18421 cgcccggttt ggtctttccc cagttgcctg gggaaaatcc tcactaagct tcatcacgca
    18481 aacgcgtgat tacttagcga tgaccttgcg gccgcggtag taaccgtcgg cagtgatgtg
    18541 gtgacgcagg tgtttttcac cagaagtttt gtctacagac aggctggtga ctgcggtcag
    18601 cgcgtcatgg gaacgacgca tgccacgttt ggaacgggtt ggtttattct gttgtacggc
    18661 catggacctt actcctcaat tacggtttat ccccgatcca acggggagca ccaattactt
    18721 acgctttaag ctggctaata cggcaaatgg gtttggcttt tgcgcttctt caggcagttc
    18781 accaaagacc atgtccgctt cggacacttc acagtgttca gaatcatgca ccggaactac
    18841 cggcaaggcg aggatgattt catcttcaac cattgcaagc agatcgattt caccgaattc
    18901 gttaacctca atcggttcat acgcttccgg cagtgcttca gcctgttcgt ctgaacgcac
    18961 aggactaaaa caatacgttg tgtagacctg atgagtaaac ggcttcccgc aacgctgaca
    19021 ctcgagcgtt accgtcacct tcgcatcgcc gtttaacact gcgagacgtt ggttatcgat
    19081 agcgaacgac atggagcatt ccacatcact gtccacactg actacggatt cggcgacgcg
    19141 ctcaacctga tcaggggtat agataccctg gtaatcaagg cgtttttgag ccgtacgaac
    19201 cggatcgaga gtcaggggta attttacctt ttgcataggg cgcgcatatt aactttgtaa
    19261 cgtcatagag tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa ttcgcacaca
    19321 ttgcgggtta ctgctttatt ttcgttcagt gctgataacg aacatcaacc tgcaaaacga
    19381 agcgcatagt ttaaaatggc tatcatcaat acgctatatc tgatggaaaa aaatatgcct
    19441 aaacttattt tagcctccac ctcgccatgg cgtcgcgctc ttctggaaaa actgcaaatc
    19501 tcttttgaat gtgcagcacc agaggtcgac gaaaccccac gcagcgacga atcaccgcga
    19561 cagttggtgc ttcgactggc acaagaaaaa gcgcaatctc tggcgtcacg ttatccggat
    19621 catttaatta ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac cggtaaaccg
    19681 ttaacggaag aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat cgtcaccctc
    19741 tatactggac tggcgttgtt taattcggcg aatgggcatc tgcaaacaga agtggagcct
    19801 tttgacgtcc atttccgtca cctgagcgag gcggagattg ataattacgt gcgtaaagag
    19861 catcccctgc actgcgcggg tagctttaag agtgaaggat ttggcattac gctgtttgag
    19921 cgtttagagg ggcgtgatcc taacacgctg gttggtttac cgctaatcgc gctgtgtcag
    19981 atgttacgtc gggaagggaa aaacccgctg atgggatagt tttttgcgcc ggatgtggtt
    20041 acgtcgcatc cggcaatcgg tgctggcggc ctgatgcggc gtaaacgcct tatcaggcca
    20101 accgcccatt atcaggttta tatgcttgta ttagcgcgcg ttacgcagct tttgcaaaca
    20161 acgcttcaaa ccttcatcca tcggagcttc gatacgcatc acctcaccgg tccccggatg
    20221 agtaaacttc aacgctgcag cgtgcaggaa caggcgattt aatcccgtgc ctgcttcagt
    20281 gagctgcctg tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg gatgacccgc
    20341 atattgtgta tgcacacgga tctggtgagt acgccctgtt accgggctac aacgcaccag
    20401 ggtggcaaat gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt tgccttcctg
    20461 actcacacgc acgatacgtt cgccgctttg cagaatattt ttcagtaacg gtgcttgaac
    20521 gctcttcaca tgcgactgcc actgaccgcg caccagcgcc aggtaatctt tttgcatccc
    20581 tttttcacgt aattgctcat gcagagaacg caacgccgag cgttttttcg ctaccagcaa
    20641 aacacctgag gtgtcccggt caagacgatg aaccagttca aggaaccgcg cttccgggcg
    20701 caacgcccgc aaaccttcaa taacgccgaa gcttaaacca ctgccgccat gtaccgccgt
    20761 accggaaggt ttattcagca ccaggatgtg atcatcttca tataagatga cgtccgccag
    20821 cgccgccacc ttttgcagat gtggcgaaac cgcctcttct tcccgctcag caacgcgaac
    20881 cggtggaata cgcacctcat cacccgcttc gagtttatat tcaggcttaa tacgtttttt
    20941 gttcacccgc acttcgcctt tacgcaaaat acggtaaatc atactttttg gtacgccttt
    21001 caattgggta cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg tgatagcaac
    21061 aatttttacg gatggagtct ctgttttcat ggttggcgat tctaattagc caacaggatt
    21121 cgcgccactc atttttctat gcttatattt acttttgcac cttattactt cactgcgtga
    21181 tcactttatt gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag
    21241 cgggtgataa atggtaaaag tcatcttgct ataacaaggc ttgcagtgga ataatgaggc
    21301 cgtttccgtg tccatccttg ttaaaacaag aaattttacg gaataaccca ttttgcccga
    21361 ccgatcatcc acgcagcaat ggcgtaagac gtattgatct ttcaggcagt tagcgggctg
    21421 cgggttgcag tccttaccgg tagatggaaa tatttctgga gagtaatacc cagtctgttt
    21481 ctttgataat tgcgctgttt ttccgcatga aaaacgggca accgacactc tgcgcctctt
    21541 tgagctgacg ataaccgtga ggttggcgac gcgactagac acgaggccat cggttcacac
    21601 ccggaaaggc gttactttgc ccgcagctta gtcgtcaatg taagaataat gagtaagtta
    21661 cgatgaaaag aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag
    21721 atgggcagcg tctgtatgac ctggatatcg aaagtccagg gcacgagcag aaaaaggcaa
    21781 acatctacaa aggtaaaatc acccgcattg aaccgagtct ggaagctgct tttgttgatt
    21841 acggcgctga acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa tatttccctg
    21901 ctaactacag tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggaag
    21961 tcattgttca gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta accaccttta
    22021 tcagtctggc gggtagctat ctggttctga tgccgaacaa cccgcgcgcg ggtggcattt
    22081 ctcgccgtat cgaaggcgac gaccgtaccg aattaaaaga agcactggcg agccttgaac
    22141 tgccggaagg catgggactt atcgtgcgca ccgctggcgt cggcaaatct gctgaggcgc
    22201 tgcaatggga tttaagcttc cgtctgaaac actgggaagc catcaaaaaa gccgctgaaa
    22261 gccgcccggc tccgttcctg attcatcagg agagcaacgt aatcgttcgc gcattccgcg
    22321 attacttacg tcaggacatc ggcgaaatcc ttatcgataa cccgaaagtg ctcgaactgg
    22381 cacgtcagca tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc aaactgtaca
    22441 ccggcgagat cccgctgttc agccactacc agatcgagtc gcagatcgaa tccgccttcc
    22501 agcgtgaagt ccgtctgccg tccggtggtt ccattgttat cgacagcacc gaagcgttaa
    22561 ccgccatcga catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa gaaactgcgt
    22621 ttaacaccaa cctcgaagct gccgatgaga ttgctcgtca gctgcgcctg cgtgacctcg
    22681 gcggcctgat tgttatcgac ttcatcgaca tgacgcctgt acgccaccag cgtgcggtag
    22741 aaaaccgtct gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc agccatattt
    22801 ctcgctttgg cctgctggaa atgtcccgtc agcgcctgag cccatcactg ggtgaatcca
    22861 gtcatcacgt ttgtccgcgt tgttctggta ctggcaccgt gcgtgacaac gaatcgctgt
    22921 cgctctctat tctgcgtctg atcgaagaag aagcgctgaa agagaacacc caggaagttc
    22981 acgccattgt tcctgtgcca atcgcttctt acctgctgaa tgaaaaacgt tctgcggtaa
    23041 atgccattga aactcgtcag gacggtgtgc gctgtgtaat tgtgccaaac gatcagatgg
    23101 aaaccccgca ctaccacgtg ctgcgcgtgc gtaaagggga agaaacccca accttaagct
    23161 acatgctgcc gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg
    23221 aacgtaagcg tccggaacaa cctgcgctgg caacctttgc catgccggat gtgccgccag
    23281 cgccaacgcc agctgaacct gccgcgcctg ttgtagcccc agcaccgaaa gctgcaccgg
    23341 caacaccagc aactcctgca caacctgggc tgttgagccg cttcttcggc gcactgaaag
    23401 cgctgttcag cggtagtgaa gaaaccaaac cgaccgagca accagcaccg aaagcagaag
    23461 cgaaaccgga acgtcaacag gatcgtcgca agcctcgtca gaacaaccgc cgtgaccgta
    23521 atgagcgccg cgacacccgt agtgaacgta ctgaaggcag cgataatcgc gaagaaaacc
    23581 gtcgtaatcg tcgccaggca cagcagcaga ctgccgagac gcgtgagagc cgtcagcaag
    23641 ctgaggtaac ggaaaaagca cgtaccaccg acgagcagca agcgccgcgt cgtgaacgta
    23701 gccgccgccg taacgatgat aaacgtcagg cgcaacaaga agcgaaggcg ctgaatgttg
    23761 aagagcaatc tgttcaggaa accgaacagg aagaacgtgt acgtccggtt cagccgcgtc
    23821 gtaaacagcg tcagctcaat cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg
    23881 tagtcgcacc ggtggttgaa gaaactgccg ctgccgaacc aattgttcag gaagctccag
    23941 ctccacgcac agaactggtg aaagtcccgc tgccagtcgt agcgcaaact gcaccagaac
    24001 agcaagaaga gaacaatgct gataaccgtg acaacggtgg catgccgcgt cgttctcgcc
    24061 gctcgcctcg tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt
    24121 atccaaccca gtcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa ctggcctctg
    24181 gcaaagtctg gatccgctat ccaattgtac gtccgcaaga tgtacaggtt gaagagcagc
    24241 gcgaacagga agaagtacaa gtgcagccga tggtgactga ggtccctgtc gccgccgctg
    24301 tcgaacctgt tgttagcgcg ccagttgttg aagaaatggc tgaagtcgta gaagcccccg
    24361 taccggttgc cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa gtgattgccg
    24421 ccgcggtaac tgaacagccg caggtgatta ccgagtctga tgttgccgta gcccagaaag
    24481 tcgcagaaca cgcagaaccg gtggttgaac cgcaggaaga gacggcagat attgaagaag
    24541 ttgccgaaac tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc
    24601 cagtcgtcgc tgaagtcgca gcagaagttg aaacggtagc cgcggttgaa ccagagatca
    24661 ccgttgagca taaccacgct accgcgccaa tgacgcgcgc tccggcaccg gaatatgttc
    24721 cggaggcacc gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg
    24781 ccgcaggtgg tcatacggca acgcatcatg cctctgccgc tcctgcgcgt ccgcaacctg
    24841 ttgagtaata attagctcaa agtaatcaag ccctggtaac tgccagggct tttttatttc
    24901 atgtttgaat caacatatta aaattcatca aatggcatcc ttgctaacca acaatgcaaa
    24961 ataggcaaaa aaaatgcccg ccccggcgat cccagagcgg gcagacaaaa catacccagt
    25021 ttcatgatat gttttaaagc gagcttattt gttgagctgg aagagcgaca atccctgcat
    25081 atcggtaaat gctttatacg atgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat
    25141 agttgcattc cagtccacat caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg
    25201 atcgctacct aaggaatcca gcgactccag ttcgttcagc tgcgtgccta attccgcgcg
    25261 aacggtaagg acattattca gcgagttttt cagtccacgg ttggttttat ccaatgccgc
    25321 agcggcgatt tctttatccg cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc
    25381 gctatccagc atggcaaaaa gattggtttc agaagcgcta ccgtctggtt ccgctaccgc
    25441 gttactggta atactgtcga aaattttgtc gcccgtatgt cctatcacca tcgaacgcga
    25501 agcatcgacc tgttgcttaa tactttctgt tccgccgaca taatcaccgt tagcctcact
    25561 aaacggcgca gtctctgttt tataaccggc aaaaatgtag cgcccattac cgtcagtggt
    25621 gttcgccaga ttcagcaact ggtcacgaaa cccctgaata tccgtagcca gcgaggcccg
    25681 gtcatcgtca ctcaaggtgc cattgctggc gtagacaatt ttttcctgag cattctggat
    25741 agcagtggtg acctggctaa gtacactctc ttccagtgac actttttgag tggcgaaagt
    25801 acgtgccagc gtgtactggc tgttttgcgc ctgtgcctgg gagagaacaa cagcttgtga
    25861 tgcagcaatg ggatcgtctg aagggttaac gactcgctta cccgtcgaca tctgctcgcc
    25921 gtacttcatc cattctgcct gagaattggt gatgccgcgc atgttttgct ggtacatcat
    25981 ctgagtactg aaacgcattt tatctcccct tagcgaatgt taatcagcgc atcaaaaatc
    26041 gcgtttgccg tctgcagaac ctgcgcattc gccaggtaat actgctgaaa acgttgcaga
    26101 tttccgtact cctcatcgag attgacaccg gaaatcgact gctgctgatt ggaaagctgc
    26161 gtcaccacat taccttgcgt ggcgctactg gttttcaacg tcgcggtttt attaccgata
    26221 tcactcacta acgaggcata agcgtcgtta aaggatttcg caccgcccac cgttttactg
    26281 ttgctttgca gatccagcag ggcctgaccg ttgcggttat cgctatcacc cgcatcttct
    26341 tcgctcgcca tcgctatttt cgcttcgtcg gtgattaata catccatgtt gacgatagcg
    26401 tcacttactg gtttcagcgt gaagctgtcg ttaacagcag gcgttcctgt aaacgtcaac
    26461 tccagaccat caaatgccac tttaccgttg gcatccggcg tcaccgtaaa agtggtatta
    26521 ctggcaaggc gggtgacctg ccactgatta ttatcgaacg agattttgta atcagtcgcc
    26581 agtaccgcgg aggcatcagt taccgtggca ccgatcgcaa cgtcacctgt gtttttcgtg
    26641 ttttgcagaa ccgcgggctt accgatagca aagaaatctt caccagcatc gccgttggca
    26701 tcaaatccgg ctttgtgttg gctgttgaaa gcctcggcaa atgccagcgc cagttgtcca
    26761 agcgtattac gcgtctggtc cagatcctga gaacggaatg tcagaatgcc gcccagcgac
    26821 ccggtattca gtaatttctc cgggatctca atattgcctg ccgtcccatc aacgtaagcg
    26881 acagtcgtac gagaagggtc ggcgctggaa ggaactgccg ccagttgccg cgccgtactt
    26941 ccctgaacca gtgagtaacc attggccatc gtgatgttat aagtaccgcc atcctgaacg
    27001 ctgacttcta cgccaacaat ctggtttaat tcgctcacca gttgatcgcg ttgatccagc
    27061 agattgttag gtgacgcccc tgcccccacg cctgtcaggc gcgagatttg atcgttcagg
    27121 ctggcaattt gtttagcgta attgttgatc tgatcaacgc tggcacctat cgcgatattg
    27181 acctgtttgt cctggtcgcg cagatattga tcggtggttt taaactgatt caccaaccct
    27241 tctgatttcc caatcagcgc ctggcgcgct gccgggtctt ccgcgttact caccagcgtt
    27301 tgcaggctgg tgaagaaatc ctgcatctgt gttgccagcg aagaggtact ggtggagagc
    27361 atattgtcga ttttcgacat ctgctcatag cgggcagtca ggccgctact ttgcgtctgc
    27421 gccgcacgta actggttggt aataaaagca tcatactcac gctgcacacc agaaacgtag
    27481 acaccattgc caacccagcc gccagcgccc aacgtgctat tggcctgcgc cataatagtg
    27541 gtttggcggg tatatccggc aacgttatag ctggagatat tattacttgc cgtatttaac
    27601 gccgcctggg ccgcgttcag tccgctcatg gcgttattga tcaagctgga catggaggtt
    27661 ccttataagc cttcaattac agagtattat cggcagcgac ccgccggact tgagttattc
    27721 agaacagatt atcaatgttc atgctgtagg ttttgctcac cttgtcgctt atcgatttca
    27781 tctgctggat catgttggtg agtttacggg catagtgagg atcggtagca taccccgcgt
    27841 cctgtagggc ctgcgccccc tgctccgcac tcgcggcggt cgtcacggcg gcgtagcgcg
    27901 ggttacgtgt taacaaccca acgtaatccg acaatgcttc cagatacgag ctgtagaccc
    27961 gaaatttcgc ttttactttc ttcgcttcgc cattttcata ttcagtcgtg gtgatttcag
    28021 tgactggccc tttccagttg ccagaggctt tgacaccaaa caggttatag ctcggctcgc
    28081 cgttttcgcg gcggatttgt cgttgccccc agccagattc cagcgccgcc tgagcgagga
    28141 tcaaatgatg tggcacaccg ctttgctggc ttgccagttg ggcgggcagc gagagttgcg
    28201 cgaggaatgc tttactgtca cccggcagcg aatcatcgta gttacgtggt acggcctttt
    28261 gcaccagctg agaaagcgcc tgattttgat aacgcaccac agtttcgagc gggaatttca
    28321 tcggtgctgc tggcgtgggc tcctctggca atggttgttc tggcgtcatc tgtttaacca
    28381 tcatctctgc aagccccaga cctttgcccg ccgtcatctg ttgggcaatc tgctggtcat
    28441 acatactggt atacaggcga ggtgctcgct gctgaacagg ccatcttttg gtaaagcgtc
    28501 gcgcatgctt ttcaacatca tctgcacgaa catcccttcc acctggcggg ccaccggacg
    28561 gatatttgcc gccggatctt cgcccgcttt cgcctttagt tcgttgagtg attgcgcatc
    28621 ccaggccgca cttgccagta gtttgctgtc gctgatcatc agatgatttc cagttttgcc
    28681 cgcagacatc ccgcactttg cattgattgc aaaatagaca tcagatccat cggcgtagcg
    28741 cccagcgcat tgagcgcgcg caccacgtta ttgaggctgg cgctggaacg tacgctttgc
    28801 agcgaaccgc cgctctggcg taagtcgatc tgcgtttgtg gcgttaccac ggtctgtccg
    28861 ccaccaaacg gtgtatctgg ctggctgaca ttggcctgac gattgactgt aactgagaga
    28921 ttcccctgcg ctaccgcgca gctgtcgagg gtcacttcgc gattcatcac caccgaaccg
    28981 gtgcgcgagt taatcactac tttagcgtcc tgcggggtga cattaacctg catattctgg
    29041 atatcggcaa ggaaacggac ctgggaactg ttgccacttg gtacgcgcac ctgaatagtc
    29101 cgcgcatcta acgcggtggc gctgccatat ccacgcacgc ggttgatggt gtcagcgatt
    29161 tgctgcgcca tgctgaaatc ttcgtcgtta agttgcaaat taagggtatt cccgacgcca
    29221 aactggctgg gcaattcacg ttcaataacc gcaccattgg tgatccgtcc accgttcagt
    29281 tggttcacct gaacactgct accgccagcg gaggctcctg cgccgccaac cagaatattg
    29341 ccctgcgcca gcgcatacac ctgactgtca acgcccttaa gcggtgtcat caacaacgta
    29401 cctccacgca agcttttggc attacccatg gaggaaacca ccacatcgat ggtttgcccc
    29461 tgacgtccaa acggaggaag tgacgctgtc accattaccg cagcgacgtt ttttagctgc
    29521 atattggtgc ccgtcggaac ggtaattccc agctgtgaga gcatgttatt aagcgtttgt
    29581 gtggtaaacg gcgtctgggt cgtctggtca ccggttccat ccagccccac caccaggcca
    29641 tagccaatca gtgagttttg ccttaccccc tgaacactgg tgagatcgcg aatacgctca
    29701 gcctgagccg ccgtcgtaac cagtagaaga attaatgcag agagaaattt aatcaccaca
    29761 gcctcactta cattggcgac aggttaagga agaaacgttg caaccagccc atattttgcg
    29821 cttcgttaat gtagccattg cctacgtatt caatgcgcgc atccgccacc tgagtagacg
    29881 gtacggtatt gctgccgctg atggtgcgtg gattaaccac gccagagaag cgaataaatt
    29941 cggtaccctg attaatggca atctgttttt cacccaccac atgcaggttg ccgttgacca
    30001 gtacctggtc aaccgtcacc gtcaacgtgc cgctaaaggt attgctggca ttggccccgc
    30061 cttttccgtt gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg ttaccaaaca
    30121 gcccctgcaa atagcgcggc acagtatcaa agccaaaatt agttttaccg tcacggctgg
    30181 cattcgcaga ggagcttttg ctggcgctga cgttctcctg caacacgatg gtcagcgtat
    30241 cgccaatatt gcgtggtcga cgatcttcaa acagcggttg atagccatag ttaatcggct
    30301 gagcagactg gaaaatagaa ccgttggcga cgggagtcgg accgggaacc ggttgtgcac
    30361 tggtcgctcc ctgtaccagc ggcgtggagg gtatccaggc gcagccggtt agtgaaagca
    30421 ccaacaagct ggaaatggca taagtatgcg cagcgttttt ttgcatggct atcatcttca
    30481 aaaatcaata aaccggtgaa cctgccaccg gttatgcctt agagttgcgt cagtttttgc
    30541 agcatctgat cggtggtgga caccgcttta ctgttgattt cgtaagcgcg ttgcacctga
    30601 atcatattga ccagttcttc cgccacgttg acgttagacg tttcaacata cccttgatac
    30661 agcagtcccg caccgttcag gcccggcgtg ctttcgttcg gtgcaccgga ggattgcgtt
    30721 tcggtgtaga ggttttcgcc aatgctctcc agtccggtat cattcataaa ggtggtgaga
    30781 ttgagctgcc caacctggac cggagctgcc tggccttgtt gggttacgct gaccacgcca
    30841 tcacgaccga tggtgatact taacgcattc gccggaatgg tgatcgctgg ctgcacctga
    30901 aaaccaccag ccgtcaccaa ctgcccgttc tgatccacct ggaaagagcc gtcacgggta
    30961 taggctgatg agccatctgg caacatcact tggaaaaagc cctgcccttt aatcgcgaca
    31021 tctttgctgt tgttggtctg cgacaggttg ccctggctgt gtaagcgttc agtggcgacc
    31081 gggcgtaccc ccgtgccgat ttgtaatccg gagggtaagg tggtttgttc ggaagactgt
    31141 gcccccggct gacgaatggt ttgataaagc agatcttcaa acaccgcgcg ctgacgctta
    31201 aaaccgttag tactgacgtt tgccaggttg ttggcaatga cgtccatatt ggtttgctgg
    31261 gcgtcaaggc ccgttttggc gatccataat gaactgatca taaaatgtcc tgtagtggat
    31321 tagtgacaaa gtgcatatgt cttcttgccg gatgcggcgt aaacgcctta tccggcctac
    31381 aagtgctcgc aaaatcgata aattgcgatt gcccccatag acccgataaa cttgcgcatc
    31441 gggcaactta tacttgtcat gtatcctttc aattaactca tcgacagcag ttggttggca
    31501 cggcctgcgt tatcatcgac gctgctgatc accttcatct gcatttcaaa acgccgcgcg
    31561 ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt tactgccttc cagaacaccc
    31621 gacatcacac gcaaggttgg atctgcctgc agtacaggcc cacgcgtggc ctgggtttct
    31681 gcgcttaaac gaaaaatgcc gtcgtcaccg cgctgcactt cgctgcccgt ggctttcacc
    31741 agcttcagac gccccactgg cgcaaccgta tttgccggat cgcccggatt cagcgccgag
    31801 attgtgccat cggcagctat cgtgatttcc gccccttccg gcacagcaat tggccccgcc
    31861 tcgcctatca ccggatgccc ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg
    31921 ccattacgcg tatacccttc gctgccgtca gcggtctgca cggccagcca accatcctgc
    31981 tgcaacgcaa cgtccagcgg gcgcgaggtg taatccatct tgccgggcgt catgtctgca
    32041 cccggtgttg acgccgttac cagcgtgcgc gtaggcagtg aaagcccttc caccggcact
    32101 gcgcgcaagg cgtttaactg cgcacgaaaa ccgggcgtag aggcattggc cagattactg
    32161 gcggttaccg cctgttgatt cagcgtctgg ctggctgctc ccatcgcggt ataaattgcg
    32221 tgatccattg agctatcccg tcagcgatta gcgtaagtta accagcgtgt tgaggatctg
    32281 gtcctgggtt ttaatggtct gggcgttaga ctgatagtta cgctgggcaa cgatcatatt
    32341 gaccagttct ttactgagat cgacgttgga cgcttccagc gcaccgttgg tcagggtacc
    32401 aaagttgccc gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca
    32461 gacgttgtcg ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg
    32521 ccccagcagt tgggtttgtt cgttggaata gttgccgaca accgtaccgt catcattgat
    32581 ttgataactc accagatcgc ccggtttgta gccgttctgg gtggttgcca caatgttgtt
    32641 agcgccggta ttttgctgca tggagttgag gaagctcagg ctaaacgtgg cgggatctgc
    32701 gccgttaatt gcgccagtgg taatattctc tgttggattt gaggtcagaa cgccattggc
    32761 attaaacacc agcttcattg caggctcggc tgtacctgtt ggatcactgc tatcctgggt
    32821 gtagacctgc cagttattat ccccggtctt cacaaagtag acgctcatgt catgagcatt
    32881 accctgactg tcgaaaacag tcaccgaacc ttttttgtta tagctatccg cattgctggc
    32941 gctgaatggc gtaacagtag gaagcggatc actggaattc aggttgatct gcatcgacgc
    33001 cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatgttag tcggattcgc
    33061 cccttgctga atagtcggcg gcgtaccggt tgccgggtaa ccggtcagct gtaaaccttg
    33121 catattcacc aggttacggt tttcatccag cttaaattgt ccgttacggc tgtagaacac
    33181 cgaaccgttg ctgtctacca gacggaaaaa accgttctgg ctgatagcaa cgtccagacc
    33241 acgcccggtg ttggtggtcg tgccatcggt aaagtcctga gtgataccgg caacttttac
    33301 ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa
    33361 gccgtatgtg gcggagttgg cgatattgtt gccaataaca tcgaggttgg tggcggcagc
    33421 gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg tgaaggctta
    33481 aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt
    33541 accgttgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct gggcaaccag
    33601 ttgtgtacca ccgttactgg cgctaatcgc tacgttgtaa gaaccgttcg gcgcagtgct
    33661 gccgtcagtc aacgtaccgt cccaggtgaa actgtggact ccggcggtca gttcaccaat
    33721 atcaatggtg cgcacaaccg cgccattttt atcggtgatg gtggccgtaa ctttgtctgc
    33781 cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc acagcccctt cttcactgcc
    33841 ggttcccgca agaacagtgg tgccggggat catcacgccg tgaccaatca ggttactggc
    33901 ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagcgtgg tattgagttt
    33961 ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt tgttttccat
    34021 tggattggtc gggtcctggt ttttcagctg cgccaccagc aaagtcagaa aactgctttg
    34081 taaatctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga cgccggtatt
    34141 tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg accgagcgta
    34201 agggttttca gcatcatgct tttcaccgtg ttgagcactt caacattggc ctgatagctg
    34261 cgtgacgccg acatggtgtt aaccatctcc ccgacgacat caacgtttgg cattttcacg
    34321 tagccctttg catccgccag cggattaccc ggttcataaa ccagtttgtc cggggcctga
    34381 ttttctataa catcggcaac ctttacgccg cctgtcgcag cacctggtgc agcgttaacc
    34441 tggaatacca cctgttttgc ccgatatggc tgtccatcag gaccggtcac gctatcagca
    34501 ttcgccagat tactggccgc cacgttcagg cgctgggact gggcagttaa cgccgacccg
    34561 gcgatatcaa aaatattcag cagtgccatc cgttaatttc cgctctgtaa aacgttcatc
    34621 atgcctttga tttgcccgct caacgcgcta aggctcatct ggtattgcag gctgttatcg
    34681 gcaaactggg tgcgttcgcg atccatatcg acggtattac cgtcaagcga aggctggtcc
    34741 ggaatacggt attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg aatgtgttgc
    34801 gttgaggtca tcgtcagtgc aaccacactg gttgcatccc gtccacgttg catgactttt
    34861 ttaagttcac tggcaaaatc gatatcgcgc gcctgataac caggggtatc agcattggcg
    34921 atgtttgctg ccagcacttc ctgacgctgt gcgcgcagat tgagcgcctc ttgttgaaaa
    34981 cgtaaggcgg cgtcgagctt atcgagcata tctcctccgc aggtatcaaa attctgccat
    35041 cagcttaaat gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt
    35101 tatgccgttg atgaccattg cggacaggta caattcacgt tgtagaaatg gctgggggcg
    35161 aaaatgctgg caataaaacg tagcgtggcg atcatcgcga tactgttcag tccgttaagt
    35221 gcggcgagta atctcacatc gcaattgcaa acctttttta gcgcccagct cgcagggata
    35281 agtgatgagg ttcgtgtttc tattcgtaca gcgcccagtc tactgccgcc gtgcgagcag
    35341 ccattgcttt cgatgagcaa taattcccgc ctgtggggca atgtgaatgt gttggctcgc
    35401 tgcggtaacg acaaacgata tttacaggtt aatgtacagg ccacaggaaa ttatgtggtt
    35461 gccgcgatgc ccattgtgcg gggaggaaag ctggaagctg gcaatgtcaa actgaaacgc
    35521 ggacggctgg ataccctgcc accgcgtacg gtgctggata tcaatcaact tgttgatgcc
    35581 attagcctgc gcgatctatc acccgatcaa cctatccagt taacccagtt tcgccaggca
    35641 tggcgggtaa aagcgggaca acgcgtcaat gtgatcgcca gcggtgatgg gtttagcgcc
    35701 aacgcagaag gtcaggcgct gaacaatgca gccgtcgcac agaatgcgcg ggtgcgcatg
    35761 gtatcgggac aggtagtcag cggcgttgtt gatgcagatg ggaatattct tataaacctg
    35821 taagctgtta aagattaccc gtcccttgcc gataaataag caacacatga taaaagcgcc
    35881 ctcaatgagg aataaaccat gagtattgat cgcacttcgc ctctgaagcc tgtaagcacc
    35941 gttcaaccgc gcgaaaccac tgacgcgccg gtaacgaaca gccgggcggc aaaaacaacc
    36001 gcctccacca gcaccagtgt gacgttaagc gacgcgcaag caaaactgat gcaacccggc
    36061 agcagtgata tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa
    36121 ctaaaaatgg acaccggcaa aattgccgat gcgctgatca acgaagcgca gcaagacttg
    36181 cagagtaact gatcgtatga cacgtcttgc agagatcctc gaccagatgt ccgctgtgct
    36241 taacgatctc aaaacggtaa tggatcaaga acagcaacat ctctctatgg ggcagatcaa
    36301 cggcagccag ttgcaatgga ttacagaaca aaaaagctca ctgctggcga cgctggatta
    36361 cctcgaacag ttacgcagga aagaacccaa tacagcaaat agcgctgata ttagtcaacg
    36421 ctggcagaaa attactgtga aaacgcagca actacgccaa atgaatcaac ataacggctg
    36481 gttactggaa ggacagattg agcgcaatca acaggcgctg gaaatgttga aaccgcatca
    36541 ggaaccgacg ctatatgggg cgaacggtca gacctcaaca acccatcgcg gcagtaaaaa
    36601 gatttcgatc tgaagattat ctccggcctg caatgcaggc cggaatgcat tgttacaccg
    36661 tccggcgggc aaattcttta actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg
    36721 caatccccgc cagcacgacc gccattaaac gcagcaagcg ccagggcatg gtacccaatg
    36781 accactccgg catgatatgt aacataccta aaagcacgcc agacatcacc agtaccgcca
    36841 ccaccagacg caacagaaac gccatccagc caggttgcgg ggtaaagatt ttctgcttac
    36901 gcaactgcca gtaaagcagc gaagcattca gacacgccgc cagaccaata gaaagtgaca
    36961 gcccggcatg tttcaacgga ccgataaacg ccaggttcat caattgcgtt aaaatcagcg
    37021 taacgatggc aattttcact ggcgttttaa tgtcctggcg ggaataaaag ccaggagcca
    37081 acactttcac tacaatcagg ccaatcaaac ccaccgagta ggcaattaac gcccgctggg
    37141 tcatcagcgc atcaaacgcg gtaaatttac cgtactggaa cagcgaaacg gtcaacggac
    37201 ctgaaagaat gcccaacgca accgcactcg gcagcgccaa caggaaacaa agacgcagcc
    37261 cccagtccat caaacggttg tattcatcat gattgccact ggcaaaactt ttcgacagcg
    37321 acggcagcaa aatggtgcca agcgccacgc ccagcacacc ggacggaaac tccattaagc
    37381 ggtcggcgta atacatccaa gacaccgaac cggaagcaag aaacgaggca aaaatggtgt
    37441 tgatgattaa ggagatctgg ctcacagaga cgccaaggat cgccggtccc atctgtttca
    37501 ccacgcgcat tgctccggca tcgtggaagt taatgcgcgg caggaccagc atgccgatct
    37561 tcttcaggtg cggtagctga tacaccagct gcaggacgcc gccgaccgta acagcccacg
    37621 ccagcgccag caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc
    37681 tgatgttaag cagtgttggc gcaaacgccg gaatcgagaa gcggttccac gtattcagaa
    37741 tcgctcccac cagcgacgcc agggagatca gcaagatata gggaaaggta atctttagta
    37801 gctggctggt cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga
    37861 tcacccacgg tgcggcgagc atgccagcga ccgtcacaac cgccagcgca agtgtcagca
    37921 ggccagaaac ataagagaca aagacccgcg tggcgtcttc accctgctta cttttatatt
    37981 ccgccagaat cggtacaaat gcctgggaaa atgccccttc ggcaaagata cggcgtaaca
    38041 agttaggaag tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg ccaaagattc
    38101 tggcgacaat tgcgtctcgt gcgaagccaa gcacacgcga aaacatggtc atcgagctga
    38161 cggcggccag cgattttaat aaattcatcg gtgttctaat ccagacccac gggcaaaacg
    38221 cctgcaaggc aggcgctgaa agaggcgaat agtctacctg gattatggtg aattgctacc
    38281 gccagatgtt acagggttta ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt
    38341 tgcgccagca ctgcctgttc gccggcggtt tgcggaactg tctgattttg cacacattca
    38401 atgaagtgcc gcgcacagcc gacaaatcca cgttgctcaa gcgtactctg ccaaccagga
    38461 atcggtttat gcactacgcc ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg
    38521 tcgatgagcg caccgtcagt cacggcctgc acggtttcac gctgacttcc ggcacggcga
    38581 tgcatacagg tggtgatctg caaaggaccg gccgaaaaat ggtgctcggc aaacagcatt
    38641 tcgccagcgt cgttagtcag tagcgtaccg ccatccagag aggctttgcc gcccgacaac
    38701 cacagcgcgg tatccaccac atgcagataa tcatccagca acgtgaaata aagatcgtgt
    38761 ggcccgacgc tattgctacg atgtttatcc attcttagcg aggctgcggt ggcgagttgc
    38821 gtttttaact caccgtagag tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt
    38881 tttttccgcg ccgccagttc caccagccgt tcagcatcgc gcagattttc tgccagcggt
    38941 ttatcgacac agacatgtac ccccgcattg agtaacgtac tgaccacgtc aaagtgactg
    39001 gcggtgctgg aatgtacaaa aaccgcatcg caactggcgg caaggctgga taacgaatcg
    39061 gcataaggaa tgcgccagct ttcacaaatt ggcagggctt tcgcgcgcgt aggcgaccag
    39121 gctccttgta acgtccagtc agacgctgcc gccagcaccg gtaaccacgc tttttgagca
    39181 atgccgccta atcccactac gccgatacgt aattttttca ctttaatctc ccaggtgggc
    39241 cagcaacgaa tcaagacgct gtttcagttc tgccacttcg atttccaggg cttcgacgcg
    39301 ggcctgtaaa tcaccgtcaa ccgcgctcga catatccgtt accgacggct gatcctctac
    39361 ctcaccgcta aaaagatgca tgtagcggct ttcacgttta cccggttcgc gggccagacg
    39421 caccacaaaa ggaccatctt cgcgattcgc cagttgttcc agcgtcgact ccacttccgc
    39481 catatcgctg aattcataca ttcgcgcggc gcggctgcgc agttcacccg gcgtctgggc
    39541 accacgcaat aacaacgtgg tgattaacgc cacttctgcg gcgctcagtt tcagatcgcc
    39601 aaattctgaa ttacaaaaac gttgctcata tttggtgacc cgattaccaa aaccgctcac
    39661 tgtgcgtaga taatgacgtt tgaccagatt atccagctgt tcctgcactt cagattcact
    39721 cagattcatg accggttcac ggttcgtttt ctgattacag gccgtgacta caccattgac
    39781 tgagagcggg tattgctccg gcgtggtcac ctgcttttcc agcaagcaac caatcacacg
    39841 agcttccagg gcagtaagtt gatatttcat gagattctcc ttagcggccg ggcgtccagt
    39901 ctggggtagt taatgccgtc agtacgtgat cgcgccattg tccatcaatc aacagatagt
    39961 ctttcgcata gccttctttt tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat
    40021 tgtgcggcat ataattagcc ataatgcgat gaatatgttg ggtgcgctgc atataacgaa
    40081 tggctgcggt cagggcttca aacatgagtc ctttgccctg ccatttttgc ccaatcgaat
    40141 aaccgagata gcaggcatga aaagagccgc gaacaacatt ggaaaaattg gcaacgccaa
    40201 taatctcttt ttcgtccggg tcgaataaac caaagtagaa agctgaacct tgtttatgaa
    40261 attcgttaat catccccagc ctggcctgcc agcctgatgg atagcagtgg ctttcgtcgc
    40321 gcactggctc ccagggcttg aggaaatggc gattctctgc gtaataatcc gccagacgcc
    40381 aggcatcacg atcatgcacc agacgcacga ccagtcggtc tgtggttaag cgcacttttg
    40441 gcacgttact gcgatagcca aacattctat acctactcct tcccgtaacg tctttaatac
    40501 gctgtaagta ttactatacc tgcgcaacca gaccctgtga aaagtccaaa atgccatttt
    40561 ctgcattatt cacatatttg ataaaaactc tctatcaaag cgacgttttg ataaaaaaat
    40621 attgtccccg aagtgatggg aaaagtctta gcggcaatgc agaatagaaa gtgctcatct
    40681 tttatttccc gggaggggaa atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt
    40741 tcctgctcat cgataatatg ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct
    40801 ctatccgctt cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt gctctcggtc
    40861 tacgccagtt tattcagcaa ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg
    40921 gtgccaaacc gatgattgtt accggtatgc tgatgcgcgc cgccggattc gccacaatgg
    40981 gtatcgccca cgaaccgtgg ctattgtggt tttcatgcct gctctcggga ctcggcggca
    41041 cgttgtttga tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca caacaacgtg
    41101 gtcgtttttt ctcgctgttg atgatgcagg acagtgccgg tgcggtcatt ggcgcattgt
    41161 tggggagctg gctgttgcaa tacgactttc gcctggtctg cgccacaggg gccgttttgt
    41221 ttgtgctgtg tgcggcgttc aatgcgtggt tgttaccggc atggaaactc tccaccgtac
    41281 gcacgcccgt tcgcgaaggc atgacccgcg tgatgcgtga caagagtttt gtcacctatg
    41341 tcctgacgct ggcgggttac tacatgctgg ctgtacaagt gatgctgatg ctgccaatta
    41401 tggtcaacga cgtggctggc gcaccctctg ccgttaaatg gatgtatgcc attgaagcgt
    41461 gtctgtcgtt aacgttgctc taccctatcg cccgctggag tgaaaagcat tttcgtctgg
    41521 aacaccggtt gatggctggg ctgttgataa tgtcattaag catgatgccg gtgggcatgg
    41581 tcagcggcct gcaacaactt ttcaccctga tttgtctgtt ttatatcggg tcgatcattg
    41641 ccgagcctgc gcgtgaaacc ttaagtgctt cgctggcgga cgcaagagct cgcggcagct
    41701 atatggggtt tagccgtctg ggtctggcga ttggcggcgc tattggttat atcggtggtg
    41761 gctggctgtt tgacctgggc aaatcggcgc accagccaga gcttccgtgg atgatgctgg
    41821 gaattattgg catcttcact ttccttgcgc tgggttggca gtttagtcag aaacgcgccg
    41881 cgcgtcgttt gctggaacgc gacgcctgat ttgatactcc cccctgactc ctccatactg
    41941 aaagtgaact gacagccaaa tggaggagtc acgtgaagct atacatttac gatcactgcc
    42001 cttactgcct caaagcccgc atgattttcg gcctgaaaaa tatccccgtc gaattacatg
    42061 ttctgctcaa cgacgacgca gaaacaccca cccggatggt cggtcaaaaa caggttccca
    42121 ttctgcaaaa agatgacagc cgctatatgc cagaaagcat ggatatcgtt cactatgtcg
    42181 ataaactcga cggcaaaccg ttactgaccg gcaaacgttc ccctgccatt gaagagtggc
    42241 tgcgcaaggt caatggctac gccaacaaac tgctgttgcc gcgttttgcc aaatcggcat
    42301 ttgatgagtt ttctactccc gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg
    42361 cgggtaattt tgccgacctg ctggcccact ctgacggtct gattaagaat atcagcgatg
    42421 atttgcgtgc gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg
    42481 aagatgatat tcagctattt ccgctactgc gtaatctgac gctggtagcc ggaatcaact
    42541 ggccaagccg cgttgctgat taccgcgata atatggcgaa acagacacaa atcaatttgt
    42601 tatcatcaat ggcgatttaa tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc
    42661 cgttttcagt tttttctgct ggcgagttta tcgctggtga cacatgctgc gttgtgtcag
    42721 gatattgacg cagatttcat gtcgaggact accatgaaca agtttttatt tgccgctgca
    42781 ttgatcgtca gtggcctgct cgttggctgt aatcaactca cccaatacac catcaccgaa
    42841 caagaaatta accagtcgct tgcgaaacat aataatttct caaaagatat cggtttaccc
    42901 ggcgtggctg acgcccatat tgttctgaca aacttgacca gccaaattgg tcgcgaagag
    42961 ccgaataagg ttaccctaac cggagacgcc aatctggaca tgaactccct gttcggtagt
    43021 cagaaagcga ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt
    43081 gcgatcttcc tgaaagagat ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa
    43141 acggtgatgc aaacgttgct tccctatttg aaccaggcat tacgcaatta ctttaaccag
    43201 caacctgctt acgtcctgcg cgaagatggc agtcagggcg aagcaatggc gaaaaaactg
    43261 gcgaaaggca ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga ttaatgacga
    43321 gggcgcattc gcgcccttta tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg
    43381 tccggcaaaa acatcccttc agccggagca tagagattaa tgactgcacc atcccaggta
    43441 ttaaagatcc gccgcccaga cgactggcac cttcacctcc gcgatggcga catgttaaaa
    43501 actgtcgtgc cgtataccag cgaaatttat ggacgggcta tcgtaatgcc caatctggct
    43561 ccgcccgtga ccacagttga ggctgccgtg gcgtatcgcc aacgtattct tgatgccgta
    43621 cctgccgggc acaatttcac cccattgatg acctgttatt taacagattc gctggatcct
    43681 aatgagctgg agcgcggatt taacgaaggc gtgttcaccg ctgcaaaact ttatccggca
    43741 aacgcaacca ctaactccag ccacggcgtg acgtcaattg acgcaatcat gccggtactt
    43801 gagcgcatgg aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac acatgcagat
    43861 atcgacattt ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc tctgcgccag
    43921 cgcctgactg cgctgaaagt cgtttttgag cacatcacca ccaaagatgc tgccgactat
    43981 gtccgtgacg gaaatgaacg gctggctgcc accatcactc cgcagcatct gatgtttaac
    44041 cgcaaccata tgctggttgg tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa
    44101 cgcaatattc accaacaggc attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc
    44161 ctcggtacgg attctgcgcc acatgcacgt catcgcaaag agagcagttg cggctgcgcg
    44221 ggctgcttca acgccccaac cgcgctgggc agttacgcta ccgtctttga agagatgaat
    44281 gctctgcagc actttgaagc attctgttct gtaaacggcc cgcagttcta tggcttgccg
    44341 gtcaacgaca cattcatcga actggtacgt gaagagcaac aggttgctga aagcatcgca
    44401 ctgactgatg acaccctggt gccattcctc gccggggaaa cggtacgctg gtccgttaaa
    44461 caataaaaat tgtagcttcc tgttgtcatt aggttatttt acctgtataa ataaccagta
    44521 tattcaacag ggggctatta tgcgaattga agtcaccata gcgaaaactt ctccattgcc
    44581 agctggggct attgacgccc tggctggcga actttcccgc cgtattcagt atgcgtttcc
    44641 tgataatgaa ggccacgtat cggtacgtta tgccgcagcg aataatttat cggttattgg
    44701 cgcaacaaaa gaagataaac agcgcattag cgaaattctc caggaaacgt gggaaagcgc
    44761 cgatgactgg tttgtcagcg aataatatgc agtgattttt gtttttgccg ggtcgccccg
    44821 gcttttttat tttacttaca aattttgagt acaaaatttc tacacttcct caaaataatt
    44881 gcacaatatg ctaaaaaaat atgaatgaca tgctgtttta ttgttcatat aaatcttaaa
    44941 agcagaaatt tatctgttcc cccctttaaa aattcggtga atacccttac ttattggtat
    45001 actgaagttg cttcagataa aaacgcattg aacctcgaat aacgttgtct agtaacacga
    45061 attagggggc catgatggaa aaaaataatg aagtcattca gactcatccg ctcgtagggt
    45121 gggacatcag caccgttgat agctatgatg cgctgatgtt gcgtttgcac taccagaccc
    45181 caaataagtc cgagcaggaa gggactgaag ttggtcagac gctctggtta accactgatg
    45241 ttgccagaca atttatttcg atattagaag caggaatcgc caaaattgaa tccggtgatt
    45301 tcccggtaaa cgagtatcgg cgtcattaac atgacatgac catccaccca aaaggcacct
    45361 caccggtgcc tttaccattt ctgggttgta tatctgcatt cagttaatta ctctgcttaa
    45421 accatgagag cagagagaaa aaaatgaaat acgatctcat cattattggc agcggttccg
    45481 taggcgctgc cgccgggtat tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg
    45541 cccatatgcc accgcatcaa cacggcagcc accacggcga tacgcgatta attcgccatg
    45601 cttatggtga aggcgaaaag tatgtcccgt tggtcctccg cgcgcaaacg ctgtgggatg
    45661 aactttcccg ccacaacgaa gatgatccca tttttgtacg ctctggtgtc attaacctcg
    45721 gcccggctga ctccgcattt ctcgccaacg tcgcccacag cgccgaacag tggcaactca
    45781 acgttgaaaa gctcgatgcg caagggatta tggcccgctg gccagaaata cgcgtcccgg
    45841 acaactacat cggcttattt gagactgatt ccggtttttt gcgcagcgaa ctggcgatta
    45901 aaacctggat ccaactggcg aaggaagcgg gctgtgcgca actgttcaac tgcccggtca
    45961 ccgcaattca tcatgacgat gatggcgtaa ctattgaaac ggttgacggt gagtatcagg
    46021 cgaaaaaagc gattgtctgc gcgggaacat gggtaaaaga cctgctcccg gagctgcctg
    46081 tccagcccgt acgtaaagta tttgcctggt atcaggccga tggccgctat agcgtgaaga
    46141 ataaattccc ggcgtttacc ggtgaactgc ccaatggcga tcaatattat ggttttccgg
    46201 cagaaaacga cgcgttgaag attggcaaac ataacggagg ccaggttatc cattcagcgg
    46261 atgaacgtgt tccgtttgcg gaagtggtca gcgatggttc ggaagccttc ccgttcttgc
    46321 gcaatgtatt gccgggtatc ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt
    46381 cgcctgacga agattttatt atcgataccc tacccggcca cgataataca ctgctcatta
    46441 ccggcctgag tgggcacggt tttaaatttg cgtcagtttt aggggaaata gctgccgatt
    46501 ttgcgcaaga caaaaaaagc gattttgatt tgacgccatt caggctttcc cgcttccaat
    46561 aatcatcatt acggcctcca gaatgaggcc gtttttacag gagtatttaa tgtgtcggct
    46621 gttgcactat ctcatcaata atattcgcga gcatctgatg ctttatcttt ttctctgggg
    46681 attgttggca attatggatc tgatctatgt gttttatttt tagttttccg cactgacata
    46741 aactgacaaa tatttaatct ccctttcatt tagcgtgaaa aattgaatga gattattcct
    46801 gcttctacag ca
//
LOCUS       DAFNBJ010000016        46009 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000016 DAFNBJ010000000
VERSION     DAFNBJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.016"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(84..983)
                     /gene="pgrR"
                     /locus_tag="L2X49_000915"
     CDS             complement(84..983)
                     /gene="pgrR"
                     /locus_tag="L2X49_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBN4796047.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1121..2041
                     /locus_tag="L2X49_000916"
     CDS             1121..2041
                     /locus_tag="L2X49_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN4796048.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            2112..2249
                     /locus_tag="L2X49_000917"
                     /pseudo
     CDS             2112..2249
                     /locus_tag="L2X49_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
     gene            2377..3105
                     /gene="mpaA"
                     /locus_tag="L2X49_000918"
     CDS             2377..3105
                     /gene="mpaA"
                     /locus_tag="L2X49_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBN4796049.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCANPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(3080..4045)
                     /gene="ycjG"
                     /locus_tag="L2X49_000919"
     CDS             complement(3080..4045)
                     /gene="ycjG"
                     /locus_tag="L2X49_000919"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBN4796050.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADIDGPTWLAVDVEPALQFTTGELHL"
     gene            4164..4670
                     /gene="tpx"
                     /locus_tag="L2X49_000920"
     CDS             4164..4670
                     /gene="tpx"
                     /locus_tag="L2X49_000920"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBN4796051.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(4714..6255)
                     /gene="tyrR"
                     /locus_tag="L2X49_000921"
     CDS             complement(4714..6255)
                     /gene="tyrR"
                     /locus_tag="L2X49_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBN4796052.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(6403..7464)
                     /locus_tag="L2X49_000922"
     CDS             complement(6403..7464)
                     /locus_tag="L2X49_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBN4796053.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESMLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(7461..8858)
                     /locus_tag="L2X49_000923"
     CDS             complement(7461..8858)
                     /locus_tag="L2X49_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBN4796054.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            9013..10011
                     /locus_tag="L2X49_000924"
     CDS             9013..10011
                     /locus_tag="L2X49_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBN4796055.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(10122..11027)
                     /gene="ompG"
                     /locus_tag="L2X49_000925"
     CDS             complement(10122..11027)
                     /gene="ompG"
                     /locus_tag="L2X49_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBN4796056.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11072..12154)
                     /locus_tag="L2X49_000926"
     CDS             complement(11072..12154)
                     /locus_tag="L2X49_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN4796057.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETGIALR"
     gene            complement(12168..12827)
                     /gene="pgmB"
                     /locus_tag="L2X49_000927"
     CDS             complement(12168..12827)
                     /gene="pgmB"
                     /locus_tag="L2X49_000927"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBN4796058.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            complement(12824..15091)
                     /gene="ycjT"
                     /locus_tag="L2X49_000928"
     CDS             complement(12824..15091)
                     /gene="ycjT"
                     /locus_tag="L2X49_000928"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBN4796059.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(15088..16143)
                     /locus_tag="L2X49_000929"
     CDS             complement(15088..16143)
                     /locus_tag="L2X49_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBN4796060.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLRDQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGDIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(16153..16941)
                     /locus_tag="L2X49_000930"
     CDS             complement(16153..16941)
                     /locus_tag="L2X49_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBN4796061.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(16959..18011)
                     /locus_tag="L2X49_000931"
     CDS             complement(16959..18011)
                     /locus_tag="L2X49_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBN4796062.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(18042..18884)
                     /locus_tag="L2X49_000932"
     CDS             complement(18042..18884)
                     /locus_tag="L2X49_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBN4796063.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(18871..19752)
                     /locus_tag="L2X49_000933"
     CDS             complement(18871..19752)
                     /locus_tag="L2X49_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBN4796064.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(19773..21065)
                     /locus_tag="L2X49_000934"
     CDS             complement(19773..21065)
                     /locus_tag="L2X49_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN4796065.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELVEQH"
     gene            complement(21079..22758)
                     /gene="ycjM"
                     /locus_tag="L2X49_000935"
     CDS             complement(21079..22758)
                     /gene="ycjM"
                     /locus_tag="L2X49_000935"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBN4796066.1"
                     /translation="MKQKITDHLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(22971..23285)
                     /gene="pspE"
                     /locus_tag="L2X49_000936"
     CDS             complement(22971..23285)
                     /gene="pspE"
                     /locus_tag="L2X49_000936"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBN4796067.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVCVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(23360..23581)
                     /gene="pspD"
                     /locus_tag="L2X49_000937"
     CDS             complement(23360..23581)
                     /gene="pspD"
                     /locus_tag="L2X49_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBN4796068.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(23590..23949)
                     /gene="pspC"
                     /locus_tag="L2X49_000938"
     CDS             complement(23590..23949)
                     /gene="pspC"
                     /locus_tag="L2X49_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBN4796069.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(23949..24173)
                     /gene="pspB"
                     /locus_tag="L2X49_000939"
     CDS             complement(23949..24173)
                     /gene="pspB"
                     /locus_tag="L2X49_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBN4796070.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(24227..24895)
                     /gene="pspA"
                     /locus_tag="L2X49_000940"
     CDS             complement(24227..24895)
                     /gene="pspA"
                     /locus_tag="L2X49_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBN4796071.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            25062..26039
                     /gene="pspF"
                     /locus_tag="L2X49_000941"
     CDS             25062..26039
                     /gene="pspF"
                     /locus_tag="L2X49_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBN4796072.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(26170..27435)
                     /gene="puuE"
                     /locus_tag="L2X49_000942"
     CDS             complement(26170..27435)
                     /gene="puuE"
                     /locus_tag="L2X49_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBN4796073.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(27473..28753)
                     /gene="puuB"
                     /locus_tag="L2X49_000943"
     CDS             complement(27473..28753)
                     /gene="puuB"
                     /locus_tag="L2X49_000943"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBN4796074.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKMLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLLHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(28755..30242)
                     /gene="puuC"
                     /locus_tag="L2X49_000944"
     CDS             complement(28755..30242)
                     /gene="puuC"
                     /locus_tag="L2X49_000944"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBN4796075.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(30392..30949)
                     /gene="puuR"
                     /locus_tag="L2X49_000945"
     CDS             complement(30392..30949)
                     /gene="puuR"
                     /locus_tag="L2X49_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBN4796076.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(30976..31740)
                     /gene="puuD"
                     /locus_tag="L2X49_000946"
     CDS             complement(30976..31740)
                     /gene="puuD"
                     /locus_tag="L2X49_000946"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBN4796077.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            31952..33370
                     /gene="puuA"
                     /locus_tag="L2X49_000947"
     CDS             31952..33370
                     /gene="puuA"
                     /locus_tag="L2X49_000947"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBN4796078.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            33519..33683
                     /locus_tag="L2X49_000948"
     CDS             33519..33683
                     /locus_tag="L2X49_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796079.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            33673..35058
                     /gene="puuP"
                     /locus_tag="L2X49_000949"
     CDS             33673..35058
                     /gene="puuP"
                     /locus_tag="L2X49_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBN4796080.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            35192..35437
                     /locus_tag="L2X49_000950"
     CDS             35192..35437
                     /locus_tag="L2X49_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBN4796081.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            35750..37393
                     /gene="sapA"
                     /locus_tag="L2X49_000951"
     CDS             35750..37393
                     /gene="sapA"
                     /locus_tag="L2X49_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBN4796082.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYDPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            37390..38355
                     /gene="sapB"
                     /locus_tag="L2X49_000952"
     CDS             37390..38355
                     /gene="sapB"
                     /locus_tag="L2X49_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBN4796083.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            38342..39232
                     /gene="sapC"
                     /locus_tag="L2X49_000953"
     CDS             38342..39232
                     /gene="sapC"
                     /locus_tag="L2X49_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBN4796084.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            39232..40224
                     /gene="sapD"
                     /locus_tag="L2X49_000954"
     CDS             39232..40224
                     /gene="sapD"
                     /locus_tag="L2X49_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBN4796085.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            40226..41032
                     /gene="sapF"
                     /locus_tag="L2X49_000955"
     CDS             40226..41032
                     /gene="sapF"
                     /locus_tag="L2X49_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBN4796086.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            41100..41453
                     /locus_tag="L2X49_000956"
     CDS             41100..41453
                     /locus_tag="L2X49_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBN4796087.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRHFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            41821..42609
                     /gene="fabI"
                     /locus_tag="L2X49_000957"
     CDS             41821..42609
                     /gene="fabI"
                     /locus_tag="L2X49_000957"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBN4796088.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            42754..43881
                     /locus_tag="L2X49_000958"
     CDS             42754..43881
                     /locus_tag="L2X49_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBN4796089.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            43949..45883
                     /gene="rnb"
                     /locus_tag="L2X49_000959"
     CDS             43949..45883
                     /gene="rnb"
                     /locus_tag="L2X49_000959"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBN4796090.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDTQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
BASE COUNT        10839 a        12333 c        11587 g        11250 t
ORIGIN      
        1 gaagaataaa aaataaccaa aacgttgaaa ggaactgaga atacgttaag agagaaatta
       61 aattaatgta tcagcaagta gtgctaaaca gcacctttat acttcagcct atctataaat
      121 aatgagaatg ccgagccagc atgtcgacgg tgtggatagt acaggtgata accgggtaaa
      181 tctggtgtga atttatccaa aacacgtatc agtttttttt ctttaatagc ccgctcaacc
      241 tgatcataag gtagatacgc caatccatgc ccatcaattg cagcatcaat gatcaggtct
      301 atcgtattca gtaaaagctg accttccatg cgaacacgaa cttcacgtcc accgcgtatt
      361 aatctccagc gatttgctgt acccgatgtg ggaagataca aattaattgc ctgatgatct
      421 attaattgtg acactgacgt tggaacactt cggcgagaaa aataatccgg tgagccaaca
      481 atagccattg gtatatctgg cccgattcga atggcgatca tatctttatc catctcccca
      541 cccagacgga cgcctgcatc aaaacgttca gaaacgacat cggtcaaacc ataatcaatg
      601 gtgagctgaa tatcaatttc aggatgcgat ttcaggaatg tgcgcattgc tggtaacaat
      661 atcgtttttg ctgcatgttc tacagtagta atacgtattg tcccggatgg gcggttctgc
      721 agctcgctca gggatgccag ggctgaatct atgtcatgca acatagggcc aagaacagac
      781 aaaagatgct cgcccgcttc agttggaaca acgctgcgcg tggttcgcgt cagaagccgc
      841 aatcccaatc gttcttctat acgacgcact atctggctta aagctgactg cgccatgctc
      901 aggcgggctg ctgcacgagt gaagctacgc tcctctgcaa cgacgacaaa cgccatcaga
      961 tcagcgattt cttcacgctt cattcagtat ctccatgcaa tctattaatc atttaaatga
     1021 taggtctaat catgattttg ggtctaatca acttaatgat caatatctat agtgatatca
     1081 ccaaccgtta aaacgaaccg acaactccat gaggaacgga atgatgaata ataaagtcag
     1141 cttcactaac agcaataatc caaccatctc tttgtctgca gtgatctatt tccctccaaa
     1201 atttgatgaa acccgtcagt atcaggcaat tgtggtatct cacccaggtg ggggcgttaa
     1261 agaacaaacg gccggaacct atgccaaaaa actggcggaa aagggatttg tcactattgc
     1321 ttatgacgca tcttatcaag gtgaaagtgg cggcgagccg cgtcagttag aaaacccata
     1381 cattcgtact gaagacatca gtgccgtgat tgattacctg acaacgcttt cttatgtcga
     1441 caatacccga attggtgcga tgggaatttg tgccggtgca ggatatacag ccaatgctgc
     1501 aattcaggat cgccgcatca aggccatcgg taccgttagt gcggtcaata ttggttcgat
     1561 gttccgtaat ggttgggaaa acaatgtgaa atctattgat gccctgccat acgttgaagc
     1621 gggttcaaac gcccgaacca gcgatattag cagcggagaa tatgccgtta tgccactggc
     1681 accaatgaag gagtccgatg cgccgaacga agaactgcgt caggcctggg agtattacca
     1741 cacccctcgc gcgcagtatc caacagcacc gggttacgct actttgcgca gccttaacca
     1801 gattattacc tatgatgctt accatatggc ggaagtgtac ctgactcagc cgatgcagat
     1861 tgtggcgggc agccaggcag ggagcaaatg gatgagtgac gacctgtacg atcgagcatc
     1921 aagccaggat aaacgctatc acattgttga aggcgcaaac catatggatc tttatgacgg
     1981 caaagcctat gtggctgaag caatttcagt attagctccg ttctttgagg aaactctgta
     2041 atgaaacata ttcaaatccg caatagtgat atggcctggc atattgctgc caataatctg
     2101 ggataatttt catgaaaaag gtactgattc ttggtgctgg cggtcagata gcccgccatg
     2161 tgattaatca actggcagat aagcaaacca ttaaacaaac tttatttgcc cgtcagccag
     2221 caaaaatcca caaaccctat ccaactaata aaatgcaaac aacttccggc aaataaagtg
     2281 atacaagatc gatgacatag cgttgaagaa aaggatttga taatccgcaa aatttgcaca
     2341 acactgcctg taatacttcc tgaaccagag acgatcatga ccgtaacccg cccacgcgcc
     2401 gaacgcggcg catttccgcc cggaacagaa cattacggac gttcattatt gggcgcgccg
     2461 ttaatctggt ttcctgcccc tgcagccagc cgcgaaagtg gtttgattct ggctggcact
     2521 cacggtgatg aaaactcttc ggtcgtcacg ctctcctgtg ctctgcggac gttgacacct
     2581 tctttgcgcc gccatcatgt ggtgctgtgt gcgaatcctg acggctgcca gctggggtta
     2641 cgggccaatg ctaatggtgt ggatttaaac agaaactttc cggcggcgaa ctggaaggaa
     2701 ggtgaaacgg tttatcgctg gaacagcgcc gctgaagaac gtgatgtcgt attactgaca
     2761 ggtgacaaac ctggctcaga acctgaaacc caggcgctgt gccagcttat acaccgcatt
     2821 caacctgcct gggtggtctc attccacgat ccactggcct gtattgaaga tcccagacac
     2881 agcgaattag gcgaatggct ggcccaggcg tttgaattgc ctctggtaac cagcgtcggt
     2941 tatgaaacgc ctggctcttt cggcagttgg tgtgcagatc tcaacttgca ttgcatcacc
     3001 gcagagtttc cgcccatctc ctccgacgaa gccagcgaaa aatatctctt tgcgatggca
     3061 aacttgctgc gctggcatcc taaagatgca attcgcccgt cgtgaactga agcgccggtt
     3121 ccacatctac cgccagccag gtcggtccgt caatatcggc gaaactgacc tgcggcacca
     3181 gcggtaaagc ggcgctaatg gcacgagagg tacacaacat gcagcccagc atcagactga
     3241 aaccttgtgc acgcgcttca gtcgccagcg ccagcgcttc cgtcagacca ccggttttat
     3301 cgagcttaat gttaaccatc tcatagcgcc ctttcagcgc cttcagattg ctacgtgtat
     3361 gacaactctc atcagcacaa atcggcaacg gatgaataaa attctccagc gccgcatcgt
     3421 cctgcgcagg aaggggttgt tcaagcatcg caacgcctaa atccgccaat agctggcaac
     3481 gcgccgccaa cccttctgca cgccaggatt catttgcatc aacgatcagc gtcgcatcgg
     3541 gcacagctgt gcgaattgcc accatccgct cactaataag atggttatcc agcttcactt
     3601 tcagtaattt cgcgcctgcc tgccagagtg ttgatgcact attggccatc tgatcaggcg
     3661 taccgatgac taccgtctgt gcagtgataa ctgtctcggg aagcgttatg ccgatcaaat
     3721 cagccagcga ttgctgctgt tttcgcgcgg ccagatccca caatgcacaa tccagcgcat
     3781 tacgtgctgc gccggcaggg agaatttttt gcaactcctc ccgtgtcagc cctttctcta
     3841 attgcgacac gacgctcata atttgcgcca ttaccgaggc atcactttct ccataacgcg
     3901 gatacggcgt gcattcgccg gtgcctttaa taccctcttc ttccagttca accaccacca
     3961 cgcgcgcttc actgcgactt ccccgggcaa tcacaaacgg ggtatgtaag ggccaggctt
     4021 cctcaaatac cttaacggtt ctcatcttta ctccttgttg tcgcggtggt ctgcagatta
     4081 agcgcaaata gcgtttgctg tgttattgac agttagcata aactaggtgt gacgttaact
     4141 atatgtaaac aggaaagata atcatgtcac aaaccgttca tttccagggc aacccggtta
     4201 cagtcgccaa ttccatcccg caggcgggta gcaaagcgca gacttttact ctcgtggcaa
     4261 aagatctgtc tgacgtcacc ctcggtcagt ttgcgggtaa acgcaaagtg ctgaacattt
     4321 tcccgagtat tgataccggt gtttgcgccg catcagtacg taagtttaac caactggcaa
     4381 ctgagatcga caacaccgtt gtgctgtgta tctctgccga tctgccgttc gcccagtctc
     4441 gtttctgcgg cgcagaaggt ctgaacaacg ttatcaccct ctccactttc cgtaacgctg
     4501 aatttctgca agcttacggt gtggcaattg ctgatggccc actgaaaggt ctggcagcgc
     4561 gtgccgttgt ggttattgac gaaaatgaca atgtgatttt cagccagctg gtggatgaaa
     4621 tcaccaccga gccggattac gaagcagctc tggctgtact gaaagcataa tttaatatgc
     4681 ctgatggtgt tgcaccatca ggcatattcg cgcttactct tcgttcttct tctgactcag
     4741 accatattcc cgcaacttat tggcaatcgc ggtatgtgaa acgccgagac gttttgccag
     4801 tttgcgcgtg ctgggataat tgcgataaag ctgggttaat accgagcgtt caaaacggct
     4861 ggtgatttcg tccagcgaac cttccatcgc atcttcgccc acggctaccg ttgcggcgtc
     4921 ataatccggc aacaaaatat cctgtggacg cagctcataa ccgtccagtt gtgtcagtgc
     4981 gcgatagata gcgttcttta actgccgcac atttcccggc cacgcataac gcgtaagtac
     5041 agtattcagg tcagcggcca gtttcggacg cggcacgccc tgctcgtcgg caaagcgggc
     5101 gacgaacagc tcagttaacg gcatgatgtc ctgcggacag tcacgtagcg gcggcagatt
     5161 gagcgtcaac acgttcagac gataatagag atcttcacgg aacatgcctt tttgcaccag
     5221 ttcgaccaga ttcttctgcg tagcgcaaat cacccgcaca tcgacatgca cctcatggtc
     5281 ttcgccaacc cgacggaaag tgccatcatt aaggaaacgc agtaatttcg cctgcatccg
     5341 tggtgacatt tcccctattt catccaacag caccgaacca ccgttcgcct gctcaaagaa
     5401 tcctttcttc ccttccggag catgaccaaa cagttcactc tcgaccgcat cttccggtat
     5461 agacgcacag ttcagcgcca ggtaaggttt gcccgctctg gggcttgcct gatggcaggc
     5521 gtaggcaaag agatctttac ctgtacctgt gtcacccgta atcagcagcg gcgcgcttag
     5581 catcgccagt ttctgcgcct gttcgacaac atgcttcatt ttcgggctga cagcgacaat
     5641 ttgactgaag gcgctgacgt cctgggcggc gacattttgc aactggcggc ccatacgaat
     5701 cgttgatcgc aacatcacca ccgcaccggt caggacgtgt tgatcatttt catcctgaag
     5761 ataaacaggc gtaatctcca tcaggaaatt ctgcccatta ataacgacat gctcgttatg
     5821 cgaatcttgc ggttcgcttt ccagccaacg taaaaaatta aagccgttaa tcaattgtgc
     5881 ggcggtatgg ttgcgcaggc gatccaattt ttgcccaaaa agctgacagc tcgccgggtt
     5941 cgccatatcc actttgcttt tcatatcgac agagagcaca ggttcaggca acgcctccag
     6001 taacgcgctc aacgccagat gctcacgttc ggaaggcatc cacgggacag tacgcacatc
     6061 ggtaacaccc gcaatacggc gtatttcggc catcagactg ctgaaactct caaactccag
     6121 ttcagcaaaa ttgaggtaga ttcgcccaat gggatcaatc tcaataccgc gtaaatcaat
     6181 gcctcttagc acgagtagat cgagtaattc gcgggtcaga ccgagtcggt cttcacaaaa
     6241 gacttccaga cgcatgggaa ccttcacctg aaaaaagaac aattaatatg atgatatgac
     6301 actatttgga tagcaggaag gtttctgtca acaatcattg acacaaagac ggaaaaacgg
     6361 gcacttcggc gtaaagatat ccggtcagca ctcgatattg cattattttt cgctgggcgt
     6421 tttgcctttt tgcagcgttt ctttcacctg accgataagc tgacgacgga aatccccgag
     6481 gcgaggtttg tcatcgtcaa tccacggcag cgggcggcaa agctccatag ctttaatccc
     6541 gagtcgtgcc gtcagaagtc ctgcaccaat cccctgagct gcgcgggtag acaaacgagc
     6601 agcgagatct tgcgacatcc agtccatccc cacttcgcgc accagttcgc tggctccggc
     6661 aaaagcgata ttcagcaata ccagcttaaa cagacgcaaa cggctgtaat accccagttc
     6721 aatgccatac agcgtggcga tgcgattaat taaacgcaga ttgcgccagg cgataaacgc
     6781 catatcgacc aacgccagcg ggctgaccgc aatcatcaac attgattccg ccgccgaacg
     6841 gctgatttcg cgccgcgcct gggcatctaa aactggctgg accaaatgcg catacaggct
     6901 gaccacttca cggtcgtttt gcgtttcatg gattgaggca taccagcgtt gcagcgccgg
     6961 atgcgactga tcaatacccg cctgctgcgc cagtttttcg caaaatgcgc ggcctttgcc
     7021 cgtgccgtgg ctatgcaata aatcacgcgc ttcgtcgcgt tcatgggcgc gctgtcgcaa
     7081 gcgccataag cgccgccact ctgttaccac agaacctacg ccagcgccga taatcaatgc
     7141 cccagcggca catccaccca gcgccaccca gtcctgggtt tgccaggcat tcattgtcca
     7201 ctgtacaccc tgcccgacaa cgcttgcgcc aaacagagcc agcccgccca tcaccatttt
     7261 gcgccacagg ctgcgtttcg gacgtaacgc tgcgtccatt accgcttcga cttgcccctc
     7321 ttcttcctgc gcttcgtcga gcgtggccgg ggcaaaattt tgcgcctgat tttcgtcaaa
     7381 ggtctgctgc gccctgaatt taggattctg atcgacctcc agaggaccgt cgaaatcaat
     7441 acgtggtttt aacggttcgg tcatcgcaat ttatctccta ttaaaaattc cagcgcagca
     7501 tcaagacgaa tatgcggtag tggtttgtcg acatccatca cctgcggacg aaatgcctca
     7561 aactggaagc cttgcttatc ccagaacgcc tgaccaggca aacgtgcggg aacttcgcca
     7621 ggataaacag tgagcggtgc gccatcgcta agtcgattac cacgcagcgc cgggattttc
     7681 tcaccgttaa catcaataat gccgctggtg gtcgcctgaa ctgacgccag ccccaggcag
     7741 tccatgctga tcccttcgaa cgccgcattt tgccaggcat cctgaatcag ttgttgcagt
     7801 aatgaaacca tattagcgtg ctgatcgatg gtcacatggt ccgctttagt ggcagcaaac
     7861 aatagcttat cgataaccgg cgaaaacaaa cgccggaaca gggtacgctg cccgtagtga
     7921 aaactttgca tcagctgcgt cagtgccaga cgcatatcat taaatgcctg tggcccactg
     7981 ttgagaggtt gcaggcaatc caccagcaca atctggcggt caaagcgcag aaaatgattc
     8041 ttatagaacc ccttcaccac cttctcgcag taataattaa agcgctcgcg cagcattccg
     8101 gcattggtat gcttatcggc ctgcgccagt ttggactcgc cccaggtatc gacatccggc
     8161 cacgggaaga attgcagcgc gggcgcacct gccatatctc ccggcaagac aaagcgccca
     8221 ggctgaataa agtgcagccc ctgctgttta cagtggtgga gataatcggt ccacgcggcg
     8281 gcaatgtccg ccagccggtt ttcgtcggca ggcgctagcg ggtcaagtcc ttcgcacatc
     8341 attcgccatt tcgccgacca ttcgccgcgc tgaccattga gtaagcccgt catctggcgc
     8401 gaccagctta agtaatcctg cgccagcatc ggcaggtcga gcaaccattc gccgggataa
     8461 tccacaatct ccagatacag cgtggaggtg tccttgaagt ggcgtagcag cgaatcgttc
     8521 gatttaaaac gtagcgccag gcggatttca ctgacgccgc gcgttggcgt cggccaggcg
     8581 ggaggatcgc catataactg cgccagtcct tcatcatatg tgaagcgcgg aatgccaaag
     8641 tcacgctgag gaatgcgttt tacgcccagc aggcgctctt cacgcaccgc gcttaacagc
     8701 ggcaagcgtg ctccggcgtg aatattgagc aactgattga ccatcgcagt gataaacgct
     8761 gttttgccgc tgcggcttag tccggttacg gcgaggcgca gatgtctgtc gacaccccga
     8821 ttcaccagcg cattaagttc atttttaagt cgcttcattg ctgtcctgtg ctgctctgaa
     8881 gaccagaaaa aaatcattcg taaggatatt aatgtggggg ccaggccatt gattatcaac
     8941 tgcccgtgtt gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg
     9001 agttcctgac ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa
     9061 tccaccgtat cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa
     9121 gtgttacggg ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc
     9181 tcttcgggtt ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt
     9241 aatccgtttt tctctgaagt tttgcatgcc atcaccgcca aagctgaaga ggaaggtttc
     9301 gacgtgatat tgcagacgtc gcacaatccg gcagaagact tacaaaaatg tgaaagcaaa
     9361 attaagcaga aaatgattaa aggcattatt atgctaagtt cgccagcgga tgagtcattt
     9421 tttgcccaac ttgataaata cgatattcct gtcgtggtga ttggcaaagt tgaaggtcaa
     9481 tatgcccatg tttattctgt cgataccgat aattttggcg acagcattgc gttgaccgat
     9541 gcattaattg aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat
     9601 gtttcagtgg atcgggtaaa tggttataag cagagcctgg ctgcgcataa tattgcagtg
     9661 cgtgatgaat ggattgttga cggcggttat acccatgaaa cagcactgca agccgcgcgg
     9721 gaattattaa gccagtcacc gttgcctgag gcagtgtttg ctactgacag cctgaaatta
     9781 atgagtattt atcgtgcggc agcagagaaa aatattgcta ttccgcagca gttagcggtg
     9841 gtgggttata gtaatgaaac gctgtcgttt attttaacgc ctgcgccagg cggtattgat
     9901 atcccaacac aggagttagg tcaacaaagc tgtgagttat tattccgctt aatttctgga
     9961 aaaccgtcac cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct
    10021 accttgcctg aatgccggat gcgatgctag cgcatcttat ccggcctaca tttatagttc
    10081 ggataaagta ctcgtgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg
    10141 ccgacacctg cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac
    10201 gcgtattcca gcgaaacgga aagcccgttc tggaaatcat aaccgtaaaa taaacctaca
    10261 cggttaaaat catggccttc acgttcaata tcatcctgcc agtcccagtt actccagcga
    10321 tccagcccaa tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag
    10381 gcgcgaattt cttgcgtaga aaactcaccg ttattgcggc tgtcgtccat attaaagccg
    10441 cgctcgagat aatagttcac tcgcaaggca accgtttcgt tgaaggtata ttgcaggcct
    10501 gtttccgttt cgactcgggt atcagcgtaa ccggtcgtgt tcagatcgtt ggcaaattta
    10561 tacatcgata accaaccgtt gaaacgtaaa tcgtccgtca gtttcacatc ccagtctggc
    10621 gcgattttcc agcgctgcat attcgccgtg tctttacccg gttcatcaac gtagtgataa
    10681 ccataattac ggaaaccacc ggtcaggccg aaactgaaat catcgttttc gaggaactga
    10741 taatgcacct ccagctccgg gcgatcaaac cacgttccac gtttacccgc gctataatct
    10801 accggccctt cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa
    10861 tagactgaag gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt
    10921 tcgtacatcg cgccgatatt aaagtgccag tcgttctttt cctcggcctg tgcgcaggcc
    10981 attcccgcac acatcaccag tgcggtacag ggtaataact ttttcattat tgttccttgt
    11041 attatcctgg gggaatgcct tccccctgta tttagcgaag tgctattccc gtttctgcat
    11101 caaagaaatg acatttcgtc atatcaaaat gaatagtgat attttctcct gcatgataat
    11161 catttaacgc accagcacgg accactaact cgtgcccccc aaccgtggtg tagagcataa
    11221 attccgcacc ggttaattct gccacgctaa ttttggcgga aatgttattt tcctcttgcg
    11281 cgtccggatg aatatcttcc ggacgtattc ccatcacgat gggcttatgc aaacttccct
    11341 gtgttttcag aaccgctaat ttctcttcgg gaatggttaa tttaagcgtt tccgtaacga
    11401 atttatcgcc atcgatcgtg ccacgaataa aattcatcgc cggtgatcca ataaatccgg
    11461 caacaaacat attcgctggt tggttataaa ccgttttcgg cgcaccaact tgctgaacaa
    11521 tcccatcctt cataatcaca atccgcgtcg ccatggtcat cgcttcggtc tgatcgtggg
    11581 tcacgtagat catggtggta ttcagcttct ggtgcagctt gctgatctct gcgcgcattt
    11641 gcacgcgcag cttggcatca aggttagaga gcggttcatc cattaaaaac acgcccgctt
    11701 cgcgcacaat cgcccgcccg agcgccacac gctggcgttg cccgccggaa agcgcgcccg
    11761 gcttgcgttt caggtactca cgcaggccga gaatttgcgc cgcccagttc acccgctcat
    11821 caatcacctc tttggcgatt ttttgcatct tcaggccaaa cgccatgttg tcgtaaaccg
    11881 tcatatgcgg atacaacgcg tagttctgga acaccatcgc tatattgcgt gctttggctg
    11941 gaacgtcatt cattcgtttg ccgtcgatca acagatcgcc gccgctgatc tcctcaagcc
    12001 cggcaatcat gcgcagggtg gtcgacttac cgcagcccga cgggccgaca aacacgatga
    12061 actctttatc ggcaatttcc aggttgaagt ccttcaccac atgcacctgg ttatcgtaga
    12121 ttttttgaat atgttgtaac gaaagctgag ccatgttgat tcctttgcta tacgttttgc
    12181 cagaaggccg ataaccgcgg ccaggtgagt gattccgttg aaggcaacag taattgcgcc
    12241 ccggttaagc ccgcgccgat ccccaccgag cgcataccgc tggcgttaat ggcgtcaatg
    12301 cccgcctgcg catcttcaat gccgatacat gcctgcggcg gcacgcccag cccggcacag
    12361 gcggcgagaa agatttccgg gtccggtttc gagtttttaa gttgggaagc atccgcgcag
    12421 aaggtgaaaa actcgcgcag ctccagcgcc gctaaaatcg tcggcgcatt cagggagaca
    12481 gaagccagcc caaccgggat ctgctgtgca cggagatctg ccagcaaaga gcgaatgccg
    12541 ggtagaacag cgctgaccgt caactcgcgc agtgagtgga catagagcag atttttgcga
    12601 tacgccagtt gcgccctctc ctgcgagtta aagtcgccct ctttgccccc gtgttgcaga
    12661 atgcgccgca gagactcatc gcggctgatc ccttttaggg attcgttaaa ctgcgcatca
    12721 atgctgatgc caatttcagc ggcaatctgc tgccacgcct ggaaatgcag atgcgcggta
    12781 tcggtgatta caccatccag atcgaaaatt accccttgca gtttcattca tcctcctgat
    12841 gtgtggtagc ggtcccattg aagggcaaaa taaaatcacc caaacagaaa acagattctt
    12901 cagccacgga tataagctga ccgttcaaac gcagtgaaac gggcgcagaa gttcgaatcg
    12961 caatacgctg cgcgtcaaga gtgacctgta attcgcagcc ctgccagaac agagggaaag
    13021 acaactgttg ccactgctca ggtaacgccg gattgagatg caattcaccg tcacgcacgc
    13081 tcaccccggc aaaaccctga atcgccccca gccagatagc gccagttgcg gcagcatgga
    13141 taccatcatc acaactatgc ggatcagcac caagatcgat ttcagtcccc tcgcgccaga
    13201 actgataact ttgggtcagc aggccacagc gtgcggcaac aatgccatgg attgctttac
    13261 ttaatgacga gtcgtgaata gtgcgcggtt cataaaattg cagattggca agacacgatg
    13321 ccgctgagaa ctgctccggc agcatgtaat tgagcatcac cacatcagct tgtttgagga
    13381 tttgcatctc gttcacttct gcgcgtgaat aatccagcag aatggtttgc ttccccgccg
    13441 ccgctttgta tttcgccaga ttaatcgccg gcttagccat aaacgaatca tcctgcggca
    13501 aaacgccgtc gggctgaatt tctggcagcc gcagttcttt aaggaacatt tcggcgcgat
    13561 ggataaacgc atcgtcgcta cagccaaact ggcgggcaat actcagcgcc tgctggacgt
    13621 tgtaatacgc catatagctg gtgaaggcgt tattattgac atgttcggta tattcgtcag
    13681 gcccaataac atcatgaatt tccagacggt cgttaaccct caccgcgcgg ctaatccaga
    13741 actttgcagt ttccagaagt agcgccatgc cttcatgcgc aatgaaactt tcatcccccg
    13801 tggtctgcca gtattgaata accgcccagg cgatatcggc caccagatga tgttccgcct
    13861 gcgccgaggc cactttttgc cgcagcccgg tgcgaatgtt aatggcggca aattccggcg
    13921 tctcttcttc gccgctgcgc gcgctttccc acggaaatag cgcgccctgc cagccgttgc
    13981 gtcgcgcttt ctcctgcgcg cctggcaagt tgtgccagcg ataacgcagt aaacttcggg
    14041 caaccgtcgg atcgctaaac agatgaaacg gtaacaaaaa tacttctgta tcccagaaaa
    14101 cgtggccttt gtagccttcg ccggttaagc cttttgccgc aatgctgctg cgctcgtcgt
    14161 gggcaggcgt catgatgcgc agatgataaa gcgcataatc taacgcttgc tgatcgtgcg
    14221 cttcgccgcc atttaccgtg atacgacgtt tctgccacca ttgacgccag ttttctgttg
    14281 atactgcaag aagttggtcg taactctgct gcgcgcacat ttcaagctgg cgaagcgacg
    14341 cgctgcccca ctcgtctaaa acggcttgcc tgtcatcccg ccagtcgatc cacaccagtt
    14401 tttgcaacgt cactgtctcg cctgcatgaa gctgcgcact ggtatgttgc tgcaaacggc
    14461 gctctttggc ggtatagcat tgctgcacat caccgctcac catacagcaa cagctgatgg
    14521 ccacatcact gcgtccatcc tgggtggtgt agatcccctg catcagatgc tgaccaaaca
    14581 cccgcacctg ggtttcgtcg agatgttggc gaccgtggtt ggtttgcgtg gcgtcgatac
    14641 ctgttgaaat cagcactgac gcgtcggcgt ccagtggcgt aatagtgatt tccagcacaa
    14701 tgagcggcag ttggtctgcc gaaacaaagc gacggctggc gatggtgtaa cctgtgccgt
    14761 tgctggtacg ccagacaacg ctacggcgta attctccact ggcaaagtca agttcacgct
    14821 gccaggcttc gcgggataac gagaaaacct caccatttat ggcaatctcc atccccaaga
    14881 tatcaggcag gttcaccagt tcgttgattt caccttttcc cgcccgatga tacagccccg
    14941 ccagatacat ccctcgcgtc tggcgcgtgt aatcttcttc atggctggcg cgaagcccaa
    15001 gatagccgtt cccctgcgcc atcagcgatg catacttgtt cagggtatgc tggctgaaat
    15061 ggggttctga taacgttact ggcctggtca taattccaca cgcgtccctg tttctgcgga
    15121 ttgatacaac gccgccacca gttgctggat gatgtccccc tgctcggcgt cggctatcat
    15181 cacgggcttg ccctgtacgt gattgataaa ggcttccatg ctgcgcaaat ggcggttgtc
    15241 gtctgccagt tcccgttgca tcagcgtcat taattcaccg ttgttatcgg tgtagatatg
    15301 tgctggaaac agcgtcgcac cggctttatc accacaaaag ctgacgttca taatcgactg
    15361 ttcgcggatg ttgagtgcaa atgacgtttc cagccacaga atgccgccgt tatgaaattc
    15421 aatggtgcca aacagcgaat cttcgacgct ataagttgcc ggatcccact cgccaaattg
    15481 accacagctc ttttgcgtgc cgatcttttg aaagctatgc gcattcacgc ttttcaccgc
    15541 cggaaagccc agcacataca tcgcagcatc cagcatatga atgccgatgt cgatcagcgg
    15601 gccaccaccc tgcagttctt tattggtaaa gacgccccag ccaggaacgc cgcagcgacg
    15661 cagggcgcgg gcggtggtaa cgtaaatctc tcccaaaacg ccgttggtca cctggtcacg
    15721 cagctgttgc gtatcgagcg caaaacgatg gtgaaagtcg taggccagca ccttgcccag
    15781 tttgcgcgcg gtatcgcaca tttcccgcgc ctgttctggc gtcatggcgg gcggtttttc
    15841 gcacatcaca tggcagcccg cttccagtgc catcagggta tgttcgtaat gaaaacggtt
    15901 aggtgagcag acgctaacca catcaggttt caccgccagc agcatggcct gcggatcgtc
    15961 ccacacggat gcattcccgt atttttctgc cagcgcctgc gcctgggaaa ggcggctgtc
    16021 acagacagcc accagttcca gatcgttgcg ggtgcagtag tacgaagcat gaaccttatc
    16081 cgccacctgg cctgcgccta ttatcgcgac ccgcagcgga gagcttgtca ttgcactttt
    16141 cacaaagacc tcttagcagg tacgcaacca ggccaacgaa tcacggtacg cctgggcagg
    16201 atcttccgcc cggatacgcc cttcgtacac tacatagccc tgatagttat ccgcgcgcag
    16261 ctgttcaaac agcgcgtgga aatccagggt gccgctgccc ggctggtagc gatggttatc
    16321 cgcaatatgc acatgaccga gcaggtcgcg gttgtcatgc agcgcctgcg ccaggttatc
    16381 ttcttcgata ttcatgtgat agaaatcgcc gataatctgt acatgtttaa gatcgttttc
    16441 gacgatgtaa cggcgggcat cggcgagggt gttgatcata tgatcctgat agcggtttaa
    16501 cggttcgaga tacaccacgg ttccggtacg cgcggcgacc tgttccagta cgcgcaggga
    16561 atcactcacc attttgcggt cgccgtccag gctacgcggc gaggtcatcg gcggtaagcg
    16621 gaaggtaaac atgccccacg ctgccggaac gacgatacct ttacccccca cttctgccag
    16681 cgcttcgaga atgcgttcga tctgctttaa gccattaaga cgacgctctt cgataaagtc
    16741 gccaatccaa ccgtcatagc caccgcaggc ggtggtcacc ggtaaaccgg tttctttgat
    16801 tgctgctttg acttcttcga gattgttgac cagcagtttg ccgtcaatct caaaaccatc
    16861 gaagcccatc tctttgatat aacgaaattt ctccagaatg ttttccggaa agaatgcctg
    16921 attctgtgtg cctattttca ttttttcatt cctttgaatt aaaacgtaac gcccattttg
    16981 atgctctgtt ccggatgctg atcgacatac tgcatgtagc tttccgggct attggcaaag
    17041 gtcactaccg ggtcgattaa atcttcgcaa ttgagataac cgttcatcag cagttcccag
    17101 caggtttctt caatacgctt acggctccag cgcggataat ccgggttcgg ttcgctgcac
    17161 gcgcgggaga aaacaatttt ggcgttattg aaatgcgctt cgcgtccgag gttaaaacct
    17221 tcagcaaatg gtttggcaaa cgcgacataa gagatggtgc cgccataagc aaggccacgc
    17281 agcgccgatt gcagcgcgtc ggcgtaaccg ctggtttcga taatcacatc ggcaccctgc
    17341 ttgccggtca gcgttttgat ctcttttcct acgtcagtgc caatggggtt aaggcagaaa
    17401 tcagcaccgt ggcgacgggc aatatcacag cgataggcga taggatcgac gccaatcacc
    17461 accgacgcgc cagcgcgttt cgccagttgg atggctattt gaccaatcgc gccaagccct
    17521 accaccacca caaaatcacc cacgcgcacg ttggcatcgc gcacgccgct catggcaaac
    17581 tgcgccgggt cgtaacaaac ggcgtttttc cacgagctgc cttgcggcat tttgcgcagc
    17641 ttgtagttat tcaccgcgtt aatgatgacc gtctcggaga gcgggccgta gccgcatacg
    17701 ctgtcgccca ccgcgtagtc ggtaacgtcg ctgccgcact cgataatgtc gccaaccacc
    17761 atgttgccaa gctggaattt gccaaactca atgccgcgcg gcgcatctgc cggacgcgga
    17821 gtgaacatct gccattcgcc gttaaagtct tcatcgataa acgggctggc ggcgcggaag
    17881 tcgaccactt ccgttccgtg tttcggtgcg ccgaaacgca cgcggatttt cacttcattg
    17941 gctaaaatcg cccgatcttc atactcaacc agcgcagcaa cacgcggtgc tgtggctact
    18001 aactttttca tttaaaactc cttgtaaaga aactggccgc tttagccctt cacgccaccg
    18061 gcggtcaaac cactcttaat aaaacgttcc gaaagcgcat acataatcac caccggcaat
    18121 gcggtcacca gtgaggccgc catcatccgt ccccagatgt aatctggcgt actgaacagc
    18181 gcgttcaggc ccaccggtaa ggtgaaattg ctggcgctgg agaggaagat cgacgcaaac
    18241 agatagtcgt tccacgccac cataaagcag tagacgaaca cggatatcag cccggacatc
    18301 gccaacggca cggtgatgcg gaagataatt tgcagacggt tgaggccatc catcatcgcc
    18361 gcttcttcga tctcatcggg gatggtgtcg aagtagcttt tcagcataaa cacggcggtg
    18421 ggcagagttt gcgtcaccat ggtgatgatc agcgccatct cagtgtcgta aatgcccagc
    18481 gcggtgatga ttttgaacag cggcaccacc agcaaaatgc cagagaacat atacaccgtg
    18541 taaaagctgg cgttgatggt catccgacct ttaaagcgca ggcgggaaag cgcgtaagcc
    18601 ccaagaatgc cgaggaatac cgccaccacg gatgaaacca cagacaccac cagactgtta
    18661 cggaagtagt cgacaaacgg gaaaatcatc gggttaaaaa tgtcgacgta atgctccagc
    18721 gtccactgct gcggcagcag cgtaggatgc agtgagatcg cctctttcgc gcccttgaac
    18781 gaggtcatca gcatcacaaa aaatggaaac agcgtgatga tgagaaacag cgccagcccg
    18841 cagtaaaaac cgatgcgact gagtgtgcgt ttatttgttg ccattgaggt tcacccgttt
    18901 cctggtcagc agaatgacag cgaaaataat gatgaagagc accaccgaaa tcgccgccgc
    18961 tttccctaag tcattaaagg caaaggcggt tttgtagaga tagacgccga gaatatcgac
    19021 cttggtggtc agcaaataaa catccgcgaa catgtagaac atccagatgg tgcgcagggt
    19081 caccaccgtc gccaggaccg gcataatcgc gggcagcgtg acgatacgaa accgttgcca
    19141 ggcattagcg ccatccatct ctgccgcttc gtacagcgat ttgtcgatgg tctgcaaaat
    19201 cgccagaaac gagataaagg catacgggaa gtagcgccag atggcgaaca gcaccaccag
    19261 cacaaagcta ctgcccggat tatcgaacca cagcggtgcc tgctcataga gatgcagaag
    19321 atcgacgccg aggtagttca caatgccgta gccgttgttg aacatgtatt tccaggcgaa
    19381 caccagcgaa atggacggcg ttacgtagga gaggatcacc agcgaacgcg cggttttgcg
    19441 caggcggaac tcgcgattga aaaacatcgc caccgccagc ccgagcacgg tgctgcccgc
    19501 caccaccagc gcggtgtacc agaccgtcat ccacagtgag tgccagaagc cgggatcgga
    19561 gaggatacgc acatagttgc tcaccccaac aaaggttgac tcgatgttgg gattgagcgg
    19621 cagacgtaaa aaactaattt cgatattcga caccatcggc cacgccacca gaccgcccag
    19681 cagtaataag ctgggcgcga gaagcaacag cgcaaagggc atatcggaac gacctgaaaa
    19741 aagcctgttc attgcggatt cctgtcctgg gtttagtgct gttcgaccag ttcatccagc
    19801 tttttctggc tcgcttgcag cgtgccaggg agatcggctt tacccacggt gacgttatgc
    19861 accatggagc tcaccacgcc agaccccgtc acatcaccca tgcgggtaaa gtttttatcc
    19921 cctactgcac caaagacctg aatatttggc agttcaccga ttagctgatt cggtagttca
    19981 cccagcgcct taataacgtc gttgtctttc caggtggcgg tagtgacaac cgctttattc
    20041 accggcagcg cagcacctgg cgacatcatc acccagtcgg caatgttgtc tgcctgctcc
    20101 ataaaggtga caaatttttc tgctgcttcc gtctcttcgg tcttttgccc ggcggtaatg
    20161 gtcagcgagg tcaacatgcc gtagaccgca gagtttttct cggttggcac cacgaaaccg
    20221 acgtttttcg ggtcgccttc tttaatcaca gccggaagga tataggtgga gtaaatcgcc
    20281 atcggcgcgg tgccgttcat aaaggcgtct ttcacttcca tgatgtcgtt agaacccggc
    20341 atagtgttgg cagcaaggtc gcggtaatag gtcagtgcct gcatcatctc tggtgtatca
    20401 agggtgattt tgccttcggc gttaaagacg ttagcctggt tggataacgc aaactgggag
    20461 aaggattgtt ccgtcaacac gctttctgct gtaggcagcg caatgccgta ttttttattc
    20521 gccgggtcat tcagtttctg tgcaacgtcc agcagctgtt gccagttttt cggctcctca
    20581 agccccgctt ttgccagcac atctttgcga taccagatac cgccaatcca ggcgctgaca
    20641 ggaacaccgg tccatgcgct accatcttcg gtacgcacaa tacgcagtac gccatcgtaa
    20701 aacgcgcctt caccaacgtt gctgatgact gtggcaaccg ctttgcgatc gataagctgc
    20761 tctttgtcca tcactttggc gtagtcatgg ctggtttcga tcacttccgg cagcgaaccg
    20821 ctacgtgaaa gagtaatgac tttagtgtta taggcatctt cttccacggg cacttgctta
    20881 acggtgatgc cagggttttc tttttcaaaa cgggcaatca atttactgat aacggcctgg
    20941 cgctcctgct ccaccgaaga atgcataaat tcgatggata cattcgtttt attttcttct
    21001 ttacagcctg aaatcagggc gcaggaaacc agtgctgata acagcacgat ttttgattta
    21061 atcatgagat gttccttttt atttaatcca cataacctgc caggggcgca gcgttatttc
    21121 attacccaat atatcaactt cactaatcag atcccgacca tgcagattag taatatttac
    21181 atgctgaata tttttactga cattaaacaa tccagtcaga caattaccat cagcgttact
    21241 tctttgaata cgcattacgg atgaattaat cgtatcaatg gtaaaattat tatccggatg
    21301 aaactcgtta tggctgcgac gaagtgtaat taaacgcgac aactcatgat ataccgcatg
    21361 ccttaatgta gcttcatcgt tcagttctcg ggttatctct ttactgtgat attttttacg
    21421 gttaatcgca cggttatatc cgagtttttc gacacctgcg taatcattac gcgagccaag
    21481 aatactttga atatatatcg ctggcacacc ggggaaactt aacaaaatcg catgggcgag
    21541 aataaacctg gcgcaacgtt cttcatcgct actctcacgg cggcttaacg catccatata
    21601 ggtcacattt atttcatacg gactgcgtgt accgtcggga ttatttttcc agtttactaa
    21661 tgcaccttcc tgctgtaacg cctcgaccag ctctaatatt tcgctttcag gcaacaagcc
    21721 ccgtagcggg tttagcccga tgccatcgtg agaggcgagg aagttaaacc aggtggtgtt
    21781 gctggaaggt agtgtcaggt tttgcgccca cgcacaaagc gcctcaacgt tctgtttttg
    21841 caccgcatgc agcaccagtg gcggcagcga gaactggtac accatatgtg cttcgtcatc
    21901 gcctgcgcca aagtaagcaa tgttgtcttt atgcggaaca ttggtctcgg taatgatcac
    21961 tgtacctggc gcaacgttat caataatcga ccgtaacagt ttgataatca gatgtgtttt
    22021 ttccagatgg atgcagcttg ttcccggctc tttccacata aagccaacgg catccaggcg
    22081 gacatactca gcacctttcg caaggtaaca gagtaaaaca tccaccatcg ccagcaacac
    22141 ttcagggcta cggtaattca ggtcaatttg atcgtcacta aaggtggtcc ataaatggcg
    22201 cgttgaatga tcgcgcatct ggaatggcgt taataacggt aacgcacgcg ggcgagtgac
    22261 ggcgctgaga tcggtttgcg ggtcaacggc aataaaaaaa tcttcaaaac ctggatgctg
    22321 ttgtaaatag tttttaaacc attcactttt tgccgacata tggttgcaga caaaatcaaa
    22381 cattaaatga ctgcattcac cgagttgctg aatatcctgc cactcccccg cttcactggc
    22441 gacctgttga taatcaatta ccgaaaagcc atcatcagat gaccatggat aaaacggcaa
    22501 caaatgaaca tgtgaaaaaa tgctttgcag ccattgatgg taaaactgat taaatgtggg
    22561 taatggtttt aaatcattgc tgtgaaattg atcggcatag gtaattaaca cgacatcact
    22621 ttcatcccag tgttttttac gtcgctgtgt aattaatcgt ttcgcactct caagacgcgt
    22681 tacaagtttc tgtaaatgag ttgcggtaaa tgttccaccg tagatttcgt ccaggtgatc
    22741 cgtaattttc tgtttcatgt tgcctttccg tggtagcggt cccatgaaac gagagactac
    22801 tccgcagaaa ataaactgtc aattgagcac aagggtttta gtcacgcagg aaatcatttt
    22861 ttaccaggga aaaagcgtat ttgtgagctg acgcaaataa tctatgggga tgtaaataag
    22921 gtgtgacaga aaaaaaaacg gcgcataagc gccgctcatg gtgaattctt ttaacctttg
    22981 accttcggca ttgcgatgtc tttcaggcca ccggcgttct caacgtgggt atatcccatc
    23041 tcgctaagga tctcttttgc ttgccctgac tggcgtccgg cattgcaata cactttcacg
    23101 gtgtcgtttt tatccggaac ggcggtggca atgcgctctt tcacttcctt caggggaata
    23161 ttgatggccc cctgaacgtg ctcttgctga tactgctctg gaacacaaac atcgatccag
    23221 tgttcagcgg cgaaaacggg cagtgaaaac accagtgcca gagcaagtaa gcctttttta
    23281 aacatgtaag cgtccttcta tggatgaaaa ttgccatcca ttataggcct gagatttaac
    23341 aatttttgcg aaactcccct caccttttat aacgctgtgc cagtttatta gcagcccgac
    23401 ttaacagcgg ttccagcgcc acagccagca acattttcag cggtcggcga gcaactgatt
    23461 ttatcgccca gcccgctaca cccgccgggc catagcgcag tgcggtaaga agtaccagct
    23521 tgcctgctaa tttgaaacca ggctttacct tttgcccggc ctgttgccag cgagtattca
    23581 taactttcct cacagttgac ggaaacggct acgtaacgtg aaagtatcgg aagtgacata
    23641 acgttccatc tcgcgtaaac gcgtttcact tgccgccagt tcacggtcga cttcatccag
    23701 caattcgctg ctggaaggta gctgctcacc aaaggccatg ttgtccggca ttggatcaag
    23761 cgcaaatgac aaaatgatgt aagcaaccag ggtaaacagc gccagaccga agaaaatcga
    23821 cagcaccacc aggatacgca ccagttttac cggtacatca aaatagttgg caatcccggc
    23881 gcagacgccg cggaccatgc cctgctgtgg aatacgccat aattttttat tgagattaat
    23941 gcccgccatt agcgatccct ccagttcgga tgttctgcat caagaatgga ttccagcgcc
    24001 tgaatacgtt cgcgcatccg ttttgcttca tcagccagtt gcgctaatcg ctgctgctca
    24061 ctttgcgaca attcactgcg accagaacga ttgctgtaat gtagccataa ccagatcggt
    24121 aaaacaaaca gcacaaaaat ggttaacgga atagccagaa atagcgcgct cataagtact
    24181 ccttagacga tgagcggcgg cgcgttcgga cgccgcctgg atgttattat tgattgtctt
    24241 gcttcatttt ggcttttaat tgtgccagtt gctcgcttat tgcatcatcg gctttcagtt
    24301 cggcaaactg atcgtccagc gatttttgtt taccgaagct gtggctttct gcttccgctt
    24361 ccatctggtc aatacgacgt tcgaaagatt cgaaacgagc cattgcttca tccagtttgc
    24421 cactgtccag ctgacgacgc acatcgcgcg acgagtttgc cgcctgatgg cgtaacatca
    24481 atgcctgctg gcgagcgcgt gtttcgctca atttgttttc cagctcgccg atctctttct
    24541 tcatgcgtgc cagcgtatcg tccaccagcg tcacttcatg ttccagtgac ttaatcagat
    24601 cggtcagttt ctgtttttca attaacgcag cacgcgccag gtcttctcta tctttcagca
    24661 gtgccagttc ggctttttcc tgccattcaa cttcacgagc cgacgcttgt tcaatgcggc
    24721 gagtaagctg tttcttttcc gccagcgcac gcgccgaagt agaacgcact tcaaccagcg
    24781 tgtcttccat ttcctggatc atcagacgca ccagtttctg cggatcttcc gctttctcta
    24841 acagagcgtt gatgttggca ttcacgatgt cggcaaagcg agaaaaaata cccataatgt
    24901 tgtcctcttg atttctgcat tcaggattgt cctgctgaac tgttaataca atttgcgtgc
    24961 caatttttta tctttttgat ttataaagat ctgattgaag aatcaacagc aacatgccag
    25021 gatgaattag cgaattacac taacaagtgg cgaatttcat catggcagaa tacaaagata
    25081 atttactcgg tgaggcgaac agctttctcg aagtgctgga acaggtttcg catctcgcac
    25141 cgctggacaa accggtgctc atcatcggcg aacgcggcac cggtaaagag ctgatcgcca
    25201 gccgcctgca ttatctctcc tcccgttggc aagggccgtt tatttccctt aactgcgcgg
    25261 cgttaaatga aaatctgctg gattccgaac tgtttggtca cgaagcgggg gcgtttaccg
    25321 gtgcgcaaaa acgtcatcca ggaagatttg aacgtgccga cggcggtacg ctatttcttg
    25381 atgaactcgc tacagcaccg atgatggtgc aggaaaaatt attgcgcgtg attgagtacg
    25441 gtgaactgga gcgcgttggc ggcagccagc cgttgcaggt gaatgtgcgg ttggtatgcg
    25501 cgacgaatgc cgatctcccg gcgatggtca atgaaggtac ttttcgcgct gacctgctcg
    25561 accgactggc ttttgatgtt gtacaactgc cgccactacg cgaacgtgaa agcgacatta
    25621 tgttaatggc ggaacacttt gccatccaga tgtgtcggga aatcaagctg cctctgttcc
    25681 cgggttttac cgagcacgcc agagaaacat tgctgaatta tcgctggccg ggaaatattc
    25741 gtgaattgaa aaacgtggtg gaacgttcag tgtatcgcca cggcaccagc gattatccgc
    25801 ttgatgacat cattattgat ccctttaaac ggcgtccacc tgaagaagcc atcgccgttt
    25861 cagaaaacac ttcgcttcca acactgccgc tggatttacg tgagtttcag atgcagcagg
    25921 aaaaagagtt gctgcaactc agtttgcaac aagggaaata taaccagaag cgcgcagctg
    25981 aattgctggg cttaacctat catcagttcc gcgcgttgtt gaaaaagcac cagatttagc
    26041 gaagttgggc aatacaactt gccggatgcg gcgtgaacgc cttatccggc atacataatc
    26101 aatacgtcgt ttgcaggtct gataagcgca gcatatcagg cattattgcg ttgcccctgc
    26161 ttctgcgtat taatctctca gcgcatcctg caaaattttc attgccgcat cgaattgcgc
    26221 atccgggatg gtcagcggat acaggaaacg aatcacgttg ccgtatgcgc cacaggtcag
    26281 tagaagcagt ccctgcgcca gcgcgcgttg ctggattttc tgtgcaatcg ccgctgacgg
    26341 ctcgcccgtt tgcggatcgt taaactctgc cgcaatcata gaccccagac cgcgtaccgc
    26401 cgcgatggcc ggaacgcttt ctttggcatc aatcagcgtg tttgtcagac gctggcccag
    26461 ttgattcgcg cgttcgcaga gtgattcttt gtcgataatg ttgagcaccg cgtgcgcggc
    26521 agccaccgcc agcgggttac cggcgtaggt gccgccaagc ccgcccggcg cgggtgcgtc
    26581 cataatattc gcgttaccga ccacgcccga aagcggcatc ccgcccgcga ggcttttcgc
    26641 catcgtcatt aaatccggct tatcggcgta atgatccatg gcaaacagct taccggtacg
    26701 cgcaaagccg ctttgcactt catcagcaat catcacaata ccgtgctcgt cgcacaggcg
    26761 gcgaatggcg gcaaccagct cttttggcgc aacgttgaaa ccgccctcgc cctgcaccgg
    26821 ttcgaaaata atggccgcga cctgcttcgc ttcgatgtct gatttaaaca agcgttcgat
    26881 ggcgtcgagg gagtcctgtg ttgaaatgcc gtgtaaatct gacggataag gtacgtgata
    26941 caccgaacca gggaacgggc cgaagccgat tttgtacggc gcgacctttc cagttaacgc
    27001 catagtcata tacgtacgac cgtggaagcc gccgctaaac gcaatcacgc cagggcgtcc
    27061 ggtatgggcg cgggcgattt tcaccgcgtt ttccaccgct tccgcaccgg tggtgaagaa
    27121 cgcggttttg gcctgcccgc tcaccggggc aagggcgttg attttctccg ccagggtgac
    27181 gtagctttca tatggcacaa tctgatacgc ggtgtgggta aactgttgca gctgctgctc
    27241 caccgccgcg accagatcag ggtggcgatg tccggtattc agcaccgcaa tgcctgcggc
    27301 gaaatcgatg tactcgttgc cctcaacatc cttcagcgtg gcgttttcag ccgactgggc
    27361 gaagaagtta cacatcacgc caaccccgcg cggggtggca gaaagacgac gctgatggaa
    27421 ttcattgttg ctcatgtttc acaccttttg actcttcact gttaatcgga aattaaacgc
    27481 ccagacgatc gcgcaggctg taatacgccg cgcccatcgc ggtaaacggt acgcgcagcg
    27541 tgcgcccgcc ggggaacggg taatgcagca gattggcgaa ggcatcgaaa cgttcggcgt
    27601 cgccgcgcag cagttcggca atcagacgcc cggcgaggtg ggtacaggtg acgccgtggc
    27661 cgctgtagcc ctgcatgtaa tagatgttgg tatcgaggcg accaaactgc ggcatacgcg
    27721 acagggtcag caggaagttg cccgtccagc ggtaatcaat tttcacgccc ttcagctgcg
    27781 ggaaggtttt cagcattttc ggcaccacaa ggcgctcaac atcgtccggg tcgcgagcgc
    27841 catacactac gccgccgccg tacagcaggc ggttatcggc ggtaagacgg tagtaatcaa
    27901 gcagatagtt acagtcttcg acgcagtagt ttttcgggat aagggaacgg gctaaatctt
    27961 ccgacagccg ctcggtggtg atcacctgcg tgccgcacgg catgctgcgt ttcgccagct
    28021 ccggctccac tttatcgccc aggtacgcat tcccggcgac aatcacatac ttcgccgtca
    28081 cctgacctgt ggctgtacgc actacggcgg gcgtggtgtg ctgaatctgc gtcactgcgg
    28141 aaagctcata cacgcgcccg ccgttcaggc ggatagcgtc cgcttcgcca atggcgaggt
    28201 tcaacgggtg aatatgtccg ccgctgtgat ccaacagcgc gccggtgtag cggtcgctgg
    28261 ccacttcacg gcgaatggcg ttggcgtcca gcagttccag ttgtttattg ccgtagcgtt
    28321 cccagttctc tttctgctct tcgagtgtgg cgagttgttt atcattcatc gccacaaaca
    28381 gaccgccggg gcggtagtcg cagtcaatct gataacgttt gatacgttcg cggatgatct
    28441 caccaccttc gaacatcatg ctgccgagca tacgggcggt gtccatgccg tagctttttt
    28501 cgatcacatc gatgtcgcgg ctgtaggagt tcacaagctg cccgccgttg cgtccgctgg
    28561 cgccaaagcc gatgcgtgag gcttcgagaa ccactacgtc aaagcccgct tctgccagat
    28621 gcagtgcgga ggaaagcccg gtatagccgc cgccgaccac gcaaacgtcg caggaaatcg
    28681 actcattcag cgtgtcgaat ggcgcgtatt tattcgcact ggcggcgtag taactgctgg
    28741 tatgttcggt catttcaggc ctccaggctt atccagatgg ttttcagttc ggtgaatttt
    28801 tcaagggcat gcagggattt gtcgcgaccg ttgccgctct gcttatagcc gccaaacggc
    28861 acggtcatat cgccgtcgtt gtagttattg acgaagacgg aaccggcttt caggcgtcgg
    28921 ctcatgcggt gcgcgcggga gaggtcgcgc gtccataccg ccgcgccaag gccgtactgg
    28981 ctgtcgttgg caagctgtag cgcctgttct tctgatgtga aacgcgtgac caccagcacc
    29041 ggaccgaaaa tctcttcgcg gcttaaggac gcattcggat ctacatccac aaagatggtc
    29101 gggccgatgg cggcagccag cccggcgtta cggccatcca acaacagttg ccctttgctt
    29161 tcgccttccc gaataaagct atggaccgag tcggcgtggg cgcagtcgat taaggtgccc
    29221 atggtggttg cgggatcaag tggatggccc ggctgccagt tttgcgcctg ctgttttaac
    29281 agggctaaga attcatcggc gatgctctct tccagcaaca ggcgcgttcc ggcgatgcac
    29341 acctgaccct ggttgtagaa gatgcctgct gcggtggcgc ttgccgcctg ttgcaaatcc
    29401 gggcagtcag cgaaaacgat gttggcgctt ttgccgcccg cttccagcca gacgcgtttc
    29461 atgttgctgt cgcccgcatc tttcagcagc tgtttcccgg tacgggttga accggtaaag
    29521 gcgatggcat cgatatcgtt atgacgcgaa agcgcctgcc ccgcttcatg accaaaaccc
    29581 gttactacgt tcagtacgcc atccggcaga cctgcttctt tcgccagccc ggcgagacga
    29641 atcgcactga gcggtgattt ttcagacggt tttagaatca cgctgtttcc cgccgccagc
    29701 gccgggccga gtttccagca agtcagcagc agcgggaagt tccacggcac gatggcggca
    29761 attacgccga ccggttcacg cacgatcatc gccagctcat ggctactggt ggtcgccact
    29821 tcgccataca ctttgtcgat cgcttcggcg taccagcgaa tggcgcgcgc cgcgccggga
    29881 atatcatcac gcagactgtg acgaatcggt ttgccggtgt cgagagtttc cagcagtgcc
    29941 agctcttcgg cgtgggcttc cattaaatcg gcgagtttat tcagtacagc tttacgttta
    30001 gccggagaag agagtgacca gtcgccgcgt tcaaatacgc cgcgtgctgc gctcaccgca
    30061 cggtcgatat cgacgctctt gccgcgggca attttggcca gcggtgcctg ggtgaccgga
    30121 tcaacggttt caaaggtttc attttccgcc gcagcagtat attcaccgtt aataaataag
    30181 cgggtttcaa tggcgagact taacgcttta tcctgccagt aagccagatg atgaaaattc
    30241 attatgactc ctgtttcacg tctatcagat atatgcagtt tgtgaacgtc ggggcgcaga
    30301 gatccccagg ccgtatgccc gtttatttct ggcgacattc tttatggatt gccgggcgca
    30361 ttgcgcttac ccggcttaca gaacaaaaag attaaaacgt ggtgggcgta tgggcgctga
    30421 taattcggca aatacctgcc gacgtattac tgaaactgtg cgggatgccg gtattaatgg
    30481 cataactttg ccccgcgacg aggtggtaat cctgaccatt aatcgtcaga acaatttcac
    30541 cttccagtac tgtgcctatt tcctcaccct gatgcttaat tctttcccca gtggttgtgc
    30601 ccggctggta cgtttcaaag atcatcgcca gcgtgcgatt cgggttaccg ttatgaacca
    30661 gcttcattga cacaccctga ctgcccatct caattaagtc gtcctgatta atgacgacct
    30721 gcggctcatc aggtttttcc ggctcggaaa agaattccga gagtgacaga ccatacacct
    30781 tcagcagctt ttgcagcgta ctgatggcag ggctgacttt atcttgttct atcgtactga
    30841 tagcactgtg agtcagcccg gagagttcgg cggcacgacg ttgtgaaagc ccctgctgct
    30901 ggcggatttc cgacaagcgt tttcctggcg ccagtccctc atcactcata tttgcatttc
    30961 cttaaactgt agtggtcaga gtcgctgttt ttcagcgata tggtgctgac aagcggtgat
    31021 aaagccctcg aacaatatac gcgaaagcgc gtactcgcta ctgttccatt ccgggtgcca
    31081 ctgtacgccc agcgcaaaag gatgattgat gacgctaacc gcctcaacta agccatccgg
    31141 cgagcgagct tcaacacgca accgtgggct aacgaccttc gccccttgcc catgcagaga
    31201 gtttacccaa aagttgctac attcaggtaa caacgcagac agtaatcccc cctcttcaac
    31261 ctgaacttca tgcgacggtg catattgctg ttccaccggc agttcgggat cttcccgatg
    31321 ttccagcaat tcaggctgtt cgcacagctt gcgatgcaac gaaccaccgg ttgccaccac
    31381 cagttcctgt aaaccccggc agatggcgaa aatggggatg cgcctttcga gtgcggcatt
    31441 aattaacgcc atgctcagaa gatcacgccc gggatcggcg tcaggctcat cgccgttttc
    31501 accatatagg tgcggctgca cattgctggg actaccagga agataaatgc catcgagttt
    31561 cggcaaaagt tgttcaagta atgacggttc cgccagcgca tgtggtagcg caattggcaa
    31621 gccgcctgcg tggatgatgg cattcaggta cttttcttgc agagtctggg tcgcatgacc
    31681 cttaagcctg ttcctgcaca ttacgacacc gataaccgga ttgttcatta tattttccat
    31741 gctcaatctc acaaagtgga ctaaattatc gccattactg cggtttaaat gacctttatg
    31801 ttcaatattt tttcaatcta gcagtgggtt ttcatttttg caaactcaat ttaacatttg
    31861 acaaacattt agtttgcata cagattcgaa tggtggtcat tatattttac gctttgataa
    31921 cgagcggaaa acaaaccaaa ggcgaagaat catggaaacc aatatcgttg aagtagagaa
    31981 ctttgttcag cagtcagaag agaggcgggg tagcgccttt acgcaggaag tgaaacgcta
    32041 cctggagcgc tacccgaata cgcaatatgt tgatgtttta cttactgatt tgaatggttg
    32101 cttccgtgga aaacgtatcc ctgtttcaag cctgaagaag ttggagaaag ggtgttattt
    32161 cccggcctcg gtgtttgcaa tggatattct gggcaacgta gtagaagaag cgggtctggg
    32221 tcaggaaatg ggcgagccgg atcgtacttg tgttcctgtt ctcggttcct taaccccttc
    32281 tgccgccgat ccagagttta tcggtcagat gctcctgacc atggtcgatg aagatggcgc
    32341 tccctttgac gttgagccgc ggaacgttct taaccgcctg tggcagcagc tacgccagcg
    32401 cggattgttc ccggttgtag cggtagagct ggagttctat ttactggatc gtcagcgcga
    32461 cgctgaaggg tatctgcaac cgccctgcgc gcccggcacc gatgaccgca atacacaaag
    32521 ccaggtttac tccgttgata acctcaacca cttcgccgat gtgctcaatg atattgatga
    32581 actggcgcag ttacagttaa ttcccgccga cggtgcggtc gccgaagcct cgccgggtca
    32641 gtttgaaatc aacctttacc ataccgataa cgtgctggaa gcctgcgatg atgcgctggc
    32701 gctaaaacgc ctggtgcgtc tgatggcaga aaagcataag atgcacgcca cttttatggc
    32761 gaagccgtat gaagagcacg cgggcagcgg aatgcatatc catatcagta tgcaaaataa
    32821 ccgtggcgag aacgtgcttt ctgacgcgga aggcgaagat tcgccgctgc tgaaaaagat
    32881 gctcgccggg atgattgacc tgatgccgtc gtcgatggcg ctgctggcac caaacgtgaa
    32941 ctcgtatcgc cgcttccagc cgggaatgta tgtgccgacg caggcgtcgt ggggccataa
    33001 caaccgcacc gtcgccctgc gtattccgtg cggcgaccgt cataatcacc gcgtggaata
    33061 tcgcgtggcg ggtgccgatg ccaacccata tctggtgatg gcagcgattt ttgccggtat
    33121 tttgcatggc cttgataacg agctgccgtt gcaggaagaa gtcgaaggca acgggctgga
    33181 acaggaaggc ttacccttcc cgattcgcca gagcgatgcc ctgggtgagt ttatcgagaa
    33241 tgatcacctg cgccgctatt taggcgaacg gttttgccat gtgtatcacg cctgtaaaaa
    33301 tgatgagctg ttgcagtttg agcgcctcat cacagaaaca gaaattgagt ggatgttgaa
    33361 aaacgcgtaa taccccttgc ggggttgttt catcggcctg agcggagcca ggcctgacat
    33421 ttgctggtaa tccagacatt tcccggatgt cgcgttatgg agcgcgggcg gcaacgggct
    33481 tccgttaacg attaagactg taacgaggaa atgagatgat gccgatgatt aaatccccgc
    33541 atggcgaggg aggctgcgta tgcgcccctc ctgcgactga ctggactccg ccccctttgc
    33601 ttcccttgct aaacaggttt gatttcaggt cgacacgacc gcaaacctta ttacgcaggg
    33661 gaggcagcaa ttatggctat taattcacca ctgaatattg ctgcgcaacc cggcaaaacc
    33721 cgtctgcgaa aatcactgaa attgtggcag gtggtaatga tgggtctggc ctatctcacg
    33781 ccgatgaccg tatttgatac ttttggtatt gtctccggca ttagcgacgg tcacgttcca
    33841 gcgtcctatt tgctggcgct ggcgggcgtg ctgtttaccg ctatcagcta cggcaaactg
    33901 gttcgccagt ttccggaggc cggttcggcc tatacctacg cgcaaaagtc gattaatccg
    33961 cacgtcggat ttatggtcgg ctggtcatca ctgctggatt atctcttttt gccaatgatc
    34021 aacgttttgt tggcgaaaat ctatctctcc gccctcttcc cggaagtgcc gccgtgggtg
    34081 tgggtggtaa ccttcgtcgc cattttaacc gccgcgaatc tgaagagcgt caacctggtc
    34141 gctaacttca ataccctgtt tgtactggtg caaatctcca tcatggtggt gtttatcttc
    34201 ctggtggttc agggactgca taaaggagaa ggcgttggca ccgtctggtc acttcagccg
    34261 tttatcagcg agaacgcgca cctgatcccg attattaccg gtgcgacgat tgtctgtttc
    34321 tcgttcctcg gtttcgatgc ggtgaccacg ctttcggaag agacgccgga cgccgcacgc
    34381 gtgatcccga aagccatctt cctgacggcg gtatacggcg gcgttatctt tatcgcggcg
    34441 tcgttcttta tgcagctgtt ctttcccgat atcagccgct ttaaagaccc ggacgccgca
    34501 ctgcctgaaa ttgcgctgta cgtcggcggc aagctctttc agtcgatttt cctctgcacc
    34561 acgtttgtga acacattagc gtctggcctg gcttcacatg ccagcgtgtc gcgtctgctg
    34621 tatgtgatgg ggcgtgacaa tgtgtttccg gagcgcgtgt ttggctatgt gcacccaaaa
    34681 tggcggactc cggcactcaa cgtcattatg gtcgggattg tcgcgctgtc ggcgctgttc
    34741 tttgatttag tcaccgcgac agcactgatt aacttcggtg ctctggtggc gtttaccttc
    34801 gtgaatctgt cggtgtttaa tcatttctgg cggcgtaaag gaatgaataa aagctggaag
    34861 gatcacttcc actatttgct gatgccgctg gttggcgcgc tgacggtggg tgtgctgtgg
    34921 gttaacctag agtcaacgtc actgacactc ggtctggtat gggcttcgtt aggcggcgca
    34981 tatttgtggt atctgatccg ccgctatcgc aaagtgccgc tgtacgaagg tgacagaacg
    35041 ccggtgagcg aaacgtaata gccggatgcg cggcgcatta tcctgcctga gggtaacgcg
    35101 ccgcgccatt aactataaaa agatgtatga cccggatcac gagtgctatc atccgccctt
    35161 atgcttagcg cattaacccc aggggcaaat aatgaaccac gatatcccgc taaaatattt
    35221 tgatattgcc gatgagtacg cgaccgaatg cgcagagcct gtcgcagagg ctgaacgcac
    35281 accgctggcc cactacttcc agttactgct cacccgtttg atgaacaatg aagagatcag
    35341 cgaagaagcc caacatgaaa tggctgctga agcggggatt aatcccgtac gcattgatga
    35401 gatcgcagag tttttgaatc aatggggtaa tgagtaatta tttaattgca gacgatcggt
    35461 cagtatcgtt gtccggataa agcgttcacg ccgcatccgg ctaacaatga cacgcactgc
    35521 agcgtgcttt aatgggtttc tcacgatcgt accgctctgc tcctcgctaa gttgttctgt
    35581 gagttatagt tatagagaaa tcacgggtac gaatgttgaa cagatggcga cgccacctgt
    35641 tcaacgacca accacacggc cctcattacg acccctgaac acaaacggta atggcaaatg
    35701 tcattaaata ttagtaagat agcctcttta cataaaatcc cttaatatta tgcgccaggt
    35761 attatcgtct cttttggtga ttgctggact tgtgagtggt caggcaatcg ccgcgcctga
    35821 atcccccccg catgctgata tccgcgacag cggttttgtc tattgcgtca gcgggcaagt
    35881 caacaccttt aacccatcca aagcgagcag tgggttaatt gtcgataccc ttgccgccca
    35941 gttttatgat cgactgctgg atgtcgatcc ctatacctat cgcctgatgc cggaacttgc
    36001 cgaaagctgg gaagtactcg acaacggcgc gacctatcgc ttccacctgc gtcgcgatgt
    36061 tccgtttcaa aaaaccgact ggtttactcc cactcgtaaa atgaatgccg acgatgtggt
    36121 gtttaccttc cagcgaattt ttgaccgcaa caacccgtgg cataacgtca acggcagcaa
    36181 cttcccctac ttcgacagcc tgcaatttgc cgataacgtg aaaagcgtcc gcaaactgga
    36241 taatcatacc gttgagttcc gactcgccca gccggatgct tcttttttgt ggcacctcgc
    36301 aacccattat gcttcggtca tgtcggcaga atatgcccgg aagttagaga aagaagatcg
    36361 ccaggagcaa ctcgaccgtc aaccggtcgg caccggtccg tatcaattgt cggaataccg
    36421 cgccgggcaa tttattcgcc tacaacgtca tgatgacttc tggcgcggta aaccgttaat
    36481 gccgcaggtg gtggtggatt taggctccgg cggcaccgga cgtctgtcga aactcctgac
    36541 tggtgaatgc gacgttctgg cctggcctgc tgccagccag ctatccattt tgcgtgacga
    36601 cccgcgcttg cgtttaacgc tgcgtcccgg gatgaacgtc gcctatctgg catttaacac
    36661 cgccaaaccg ccgctaaata atcccgctgt ccgccatgcg ctggcactgg cgattaataa
    36721 ccagcgcctg atgcaatcca tctattatgg tacggctgaa acggcggcct ctattttacc
    36781 gcgcgcctcg tgggcctatg acaacgaggc taaaattact gaatacgatc cggcgaaatc
    36841 gcgcgaacag ttgaagtcgt tggggctgga aaatttaacg ctgaaactgt gggtgcccac
    36901 acgttcgcag gcgtggaacc ccagtccact gaaaactgcc gaactgattc aggcggatat
    36961 ggcgcaggtt ggcgtaaaag tggtgattgt gccggtagaa ggtcgctttc aggaggcgcg
    37021 gttgatggat atgagccatg atctgacgtt atccggttgg gcgacggaca gtaacgaccc
    37081 ggacagtttc ttccgtcctt tactgagctg cgcggcaatt cattcacaga ccaacctcgc
    37141 ccactggtgc gatccgaaat tcgacagcgt gttgcgtaag gcgctctcct cgcagcagct
    37201 ggcggcgcgt attgaagcct atgacgaagc gcagagtatt ctggcgcagg aattgcccat
    37261 tttgccgctg gcgtcgtcat tgcgtttgca ggcctatcgg tacgatatca aaggtctggt
    37321 acttagcccg tttggtaacg cctcctttgc tggggtgtat cgcgagaaac aggatgaggt
    37381 gaaaaaacca tgattatctt caccttacgc cgcattttgt tattgattgt caccttgttc
    37441 ctgctgacct tcgttggctt cagcttgagc tatttcaccc ctcatgcgcc gttacaaggc
    37501 gcgtcactgt ggaatgcctg ggtgttctgg tttaacggcc tgatccactg ggattttggt
    37561 gtttccagca ttaacggtca accgattgcc gaacagttaa aagaggtctt cccggcgacg
    37621 atggaactgt gcatcctcgc cttcggtttt gcgctgattg ttggcatccc cgtggggatg
    37681 attgccggga ttacgcgcca taagtggcag gataacttga tcaacgccat cgccttactg
    37741 ggcttttcaa tcccggtgtt ctggctggcg cttctgttga cgctgttttg ttcactcacg
    37801 ctaggttggt tgcccgtttc agggcgtttc gatctgctct acgaagtgaa accgattacc
    37861 ggttttgcgt tgattgatgc ctggctttcg gactcaccat ggcgggatga aatgatcatg
    37921 agcgcaatcc gccatatgat attgcccgtg atcaccctgt cggttgcgcc aacaactgaa
    37981 gtgatccgcc tgatgcgtat cagcactatc gaagtgtatg accagaacta tgtaaaagcg
    38041 gcggcgaccc gcggtttgtc gcgctttact attttgcgtc gccacgttct gcataacgcg
    38101 ttaccgccgg ttattcctcg cctgggctta cagttttcca ccatgctgac gctggcgatg
    38161 atcaccgaaa tggtttttag ctggccgggt ttagggcgct ggttaattaa cgccatccgc
    38221 cagcaggatt acgcagccat ttccgccgga gtgatggtgt gtggctcact ggtcattatt
    38281 gttaacgtga tttctgatat tttgggtgcc atggctaacc ctctgaaaca taaggaatgg
    38341 tatgccttac gatagcgtat acagcgaaaa gcgcccgccg ggcacgctgc gtaccgcctg
    38401 gcgcaaattt tatagtgacg cctctgcgat ggtcggcctg tacggctgcg cggggctggc
    38461 tgtactgtgt atttttggcg gctggtttgc gccttacggc atcgaccagc aatttctcgg
    38521 ttatcaatta ttgccgccgt catggtcgcg ctatggcgaa gtttctttct tcctggggac
    38581 tgacgacctc ggacgcgatg ttttaagccg cttactgagc ggagctgcgc caaccgtggg
    38641 tggcgcattt gtggtgacgc ttgccgcgac gatctgtggc ctggtactgg gcaccttcgc
    38701 cggggcgaca cacggcctcc gctcagcggt gcttaaccat attctggata ccttactggc
    38761 gatcccttcg ctgctgttgg caattatcgt tgtagcgttt gccggaccga gtttgtccca
    38821 cgctatgttt gctgtctggc tggcattgct gccgcgtatg gtacgttcga tttacagcat
    38881 ggtgcatgac gaactggaaa aagagtacgt tatcgccgcc cgtctggatg gcgcatcaac
    38941 gctgaatatt ctctggtttg ctgtgatgcc aaacatcacc gccgggctgg tcaccgagat
    39001 cacccgcgca ctgtcgatgg caattctcga tatcgccgcg ctgggctttc tcgatctcgg
    39061 cgcacaactc ccctcgcctg aatggggagc gatgctcggt gatgcgctgg aactgattta
    39121 tgtcgccccg tggactgtca tgctgccagg tgcggcaatt atgattagcg tgttgttggt
    39181 taacctcctt ggcgatggcg ttcgtcgtgc gattattgcg ggggtggaat aatgccatta
    39241 ctcgatattc gtaacctgac tattgaattt aaaaccgggg atgagtgggt taaagccgtt
    39301 gaccgcgtaa gtatgacctt aaccgaaggt gaaatccgcg gtcttgttgg cgaatccggt
    39361 tccggcaaaa gtttgattgc gaaagcgatt tgtggggtga ataaagataa ctggcgtgtt
    39421 actgcagacc gtatgcgttt tgatgatatc gatttgctgc gtctctccgc acgcgaacgg
    39481 cgcaagctgg ttggtcacaa cgtgtcgatg attttccagg aaccgcagtc gtgtctagat
    39541 ccttcagaac gtgtgggtcg ccagttgatg caaaacatcc cagcctggac ctataaaggc
    39601 cgttggtggc agcgttttgg ctggcgcaaa cgccgtgcca ttgaactact gcaccgcgtg
    39661 gggattaaag atcacaaaga tgcgatgcgc agttttccct atgagttgac cgaaggtgaa
    39721 tgtcaaaaag tgatgatagc cattgcactg gcgaatcaac cgcgtctgct gattgctgac
    39781 gaaccgacaa actcaatgga gccaacaacc caggcgcaaa tctttcgcct gttgacgcgt
    39841 ctcaaccaaa acagcaatac cactattttg cttatcagcc atgacttaca aatgcttagc
    39901 caatgggcgg ataaaattaa cgtgctttac tgcggtcaaa cagtggaaac cgcgccgagt
    39961 aaggagttgg tgacgatgcc acatcatcct tatacccagg cgctgatccg cgcgatacca
    40021 gacttcggca gcgcgatgcc gcataaaagt cgcctcaata cgctgcccgg cgctatccca
    40081 ctgctggaac agttaccgat tggttgtcgt ctggggccgc gttgcccgta cgcacaacga
    40141 gaatgcattg tgacgccacg tttgacgggg gcgaaaaatc atctctatgc ctgtcatttc
    40201 ccgctgaaca tggagaaaga gtgagatgat cgaaacgctg cttgaagtgc gtaatttaag
    40261 taaaacgttc cgctaccgga ccggttggtt tcgtcgtcag accgtagaag cggtaaaacc
    40321 cttgagcttt acgctacgtg aaggccagac actggcgatt attggcgaga atggttcggg
    40381 taaatccacg ctggcaaaaa tgctggcggg aatgatagaa cctaccagcg gcgagttatt
    40441 gattgacgat catccactgc attttggcga ttattccttc cgcagtcagc gtattcgcat
    40501 gatttttcag gatccttcga cctcgttgaa tccccgtcaa cgtatctcgc aaattctgga
    40561 ttttccactg cgcctgaaca ccaacctgga accggaacag cgtcgcaaac agattattga
    40621 aacgatgcgt atggttgggc tgctaccgga tcacgtcagt tactatccgc atatgttggc
    40681 ccccgggcaa aaacagcgtc tgggtctggc gcgcgcgctg atattgcgcc caaaagtcat
    40741 tattgccgat gaagccctcg cttcactgga tatgtcgatg cgttcgcagt tgattaatct
    40801 gatgctggaa ttacaggaaa aacagggcat ttcgtatatt tatgttaccc agcatatcgg
    40861 aatgatgaag cacattagcg accaggtgct ggtgatgcat cagggcgagg ttgtcgaacg
    40921 aggcagcacc gcggatgtgc tggcatcgcc gctgcatgaa ctcaccaaac ggctgattgc
    40981 cgggcacttt ggcgaagcat tgacagcgga tgcgtggcgt aaagatcgct aattttctga
    41041 atcctccttc cagattatcc acctcattct gacaagagat taaatcttgt cagaatgagg
    41101 tgatggataa aataaaatca aatgcgcgtg atttaagacg caatctcact ttgcaggaac
    41161 gaaagctctg gcgatatctt cgcagccgac attttggtga tttcaaattt cgccgtcaac
    41221 atccggtggg gagctacatt ctcgattttg cttgctgctc ggcgcgcgta gtcgttgagc
    41281 tggatggtgg gcagcatgat ttggcagttg cctatgatac caggcgcact agctggcttg
    41341 agtcgcagag ctggaccgtg ctgcgtttct ggcataacga gattgattgt aatgaggagg
    41401 cggtgctgga gattattctg caggaactga accgccggtc accctcaccc tgaaagggcg
    41461 agggggccga ccgagccgaa tagctgttgt ggtgaaaaca tggagacagt gctggagaat
    41521 attcggcaag gtctgaacca tcccagccat cgccataaaa gggttagggg ctgtatgagc
    41581 ctgtttgttg ctggggtaac aatatttgca caatatggtc ccctcgcccc tctggggaga
    41641 gggttagggt aaggggaaaa gcgcccaccc tgccgcagcc tgctccggtc ggacctggca
    41701 actatagcta ctcacagcca ggttgattat aataaccgtt tatctgttcg tactgtttac
    41761 taaaacgacg aatcgcctga ttttcaggca caacaagcat caacaataag gattaaagct
    41821 atgggttttc tttccggtaa gcgcattctg gtaaccggtg ttgccagcaa actatccatc
    41881 gcctacggta tcgctcaggc gatgcaccgc gaaggagctg aactggcatt cacctaccag
    41941 aacgacaaac tgaaaggccg cgtagaagaa tttgccgctc aattgggttc tgacatcgtt
    42001 ctgcagtgcg atgttgcaga agatgccagc atcgacacca tgttcgctga actggggaaa
    42061 gtttggccga aatttgacgg tttcgtacac tctattggtt ttgcacctgg cgatcagctg
    42121 gatggtgact atgttaacgc cgttacccgt gaaggcttca aaattgccca cgacatcagc
    42181 tcctacagct tcgttgcaat ggcaaaagct tgccgctcca tgctgaatcc gggttctgcc
    42241 ctgctgaccc tttcctacct tggcgctgag cgcgctatcc cgaactacaa cgttatgggt
    42301 ctggcaaaag cgtctctgga agcgaacgtg cgctatatgg cgaacgcgat gggtccggaa
    42361 ggtgtgcgtg ttaacgccat ctctgctggt ccgatccgta ctctggcggc ctccggtatc
    42421 aaagacttcc gcaaaatgct ggctcattgc gaagccgtta ccccgattcg ccgtaccgtt
    42481 actattgaag atgtgggtaa ctccgcggca ttcctgtgct ccgatctctc tgccggtatc
    42541 tccggtgaag tggtccacgt tgacggcggt ttcagcatcg ctgcaatgaa cgaactcgaa
    42601 ttgaaataat cattctgttg gtaaatattg ggcggcgttc tgccgcccgt tatctctgtt
    42661 atacctttct gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag
    42721 gataacgatt tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa
    42781 agccactggt atcatgaaac gcaatccagt actgcggagt atgacgttct gcctctggtc
    42841 ccggaagccg caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagaaacg
    42901 ctggctccct tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga attgttccct
    42961 gaccagctta ccgtgacccg ttcacagacc ttcaccgctt atgaacgctt gtctacggcc
    43021 ctgacggttg ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt
    43081 acgccgcttc ccgggcctga ttcctccagg gaaagcaatc ataggttggc acaaatcacg
    43141 caatatgccc gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat
    43201 ctgaatgacg tcggtcttac tgcctgggac tgtgtgatca ttaaccaaat cattggtttt
    43261 attggctttc aggcgcggac cattgcgaca tttcaggctt atctcgggca cccggtacgc
    43321 tggttacccg gtctggaaat acaaaactac gccgacgcgt cactgtttgc tgatgaatca
    43381 atacgctggc gaagcagcta tgaggtggaa aaactacctg aagagtacac aaaaagttca
    43441 acagcagaac tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt
    43501 ctcgaaaggt tgttaaacag tacacgggtt aatacacagc cggataataa gcttgcggcg
    43561 ttgttatgcg cgcggataaa tggcagtcct gcttgttttg ccgcctgtat ggattcatca
    43621 aatgaatata aaaaaatcag cccccttctg cgcaagggcg aaaatgaaat taaccaatgg
    43681 gctgaccgtc attctgttga gcacgctacc gttcaggcga tacaatggct gacccgagca
    43741 cccgatcgct ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg
    43801 cagattatta atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc
    43861 gcgttgggtg agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc
    43921 gctctaatgg ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct
    43981 taaacagcaa ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa
    44041 aggctttggc ttcctggaag tcgacacgca aaaaagttat ttcattccgc cgccgcagat
    44101 gaaaaaagtc atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga
    44161 atccgcagag ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca
    44221 gggcaaaaat gaccgtctgg ccatcgttcc tgatcatcca ctcttaaaag acgccattcc
    44281 ttgccgcgca gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga
    44341 aatgcgccgt catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat
    44401 cacttttggt gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga
    44461 aaaagaagca ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga
    44521 tctgaccgcg ctggattttg tcaccatcga cagtgccagc acagaagata tggatgacgc
    44581 ccttttcgct aaggcgttgc cggatgacaa acttcagctg attgtggcga ttgccgatcc
    44641 aaccgcgtgg attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac
    44701 caactatctg cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg
    44761 ctcactgcgc gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga
    44821 tggcaccatt gaagataata tcgaattctt tgccgccacc atcgaatcca aagcaaagct
    44881 ggtgtatgac caggtttctg actggctgga aaataccggt gactggcagc ctgaaagtga
    44941 agcaattgcc gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg
    45001 tcataaccac gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa
    45061 aggtgaagtg ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga
    45121 agcgatgatt gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg
    45181 catctataac gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct
    45241 gaaaacgcac ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa
    45301 actgcgtcgt gaactggacg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt
    45361 ccagtcattt gctgaaatta gcactgaacc cggtcctcac tttggcctcg gtctggaagc
    45421 atacgccacc tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct
    45481 gaaagcggtt atcaaaggcg aaactgcgac gcgcccacag gatgaaatca ctgtccaaat
    45541 ggccgagcgt cgccgtctca accggatggc agaacgtgat gttggtgact ggttatacgc
    45601 acgcttcctg aaagacaaag ccgggaccga cacccgtttc gcagcggaaa ttgtcgatat
    45661 cagccgtggc ggcatgcgtg ttcgtttggt tgataacggc gctatcgcct ttattccggc
    45721 acctttctta cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca
    45781 aattaaaggt gaaacggttt acaaagtgac tgacgttatt gacgtcacta ttgccgaagt
    45841 ccgcatggaa acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca
    45901 ttcctcatgg atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt
    45961 ttaactgact ttcgtttgaa aactggcgtt tttccaaaac tggattaca
//
LOCUS       DAFNBJ010000017        44581 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000017 DAFNBJ010000000
VERSION     DAFNBJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.017"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            178..1272
                     /gene="gcvT"
                     /locus_tag="L2X49_000960"
     CDS             178..1272
                     /gene="gcvT"
                     /locus_tag="L2X49_000960"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBN4796091.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1296..1685
                     /gene="gcvH"
                     /locus_tag="L2X49_000961"
     CDS             1296..1685
                     /gene="gcvH"
                     /locus_tag="L2X49_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBN4796092.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1803..4676
                     /gene="gcvP"
                     /locus_tag="L2X49_000962"
     CDS             1803..4676
                     /gene="gcvP"
                     /locus_tag="L2X49_000962"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBN4796093.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            4942..5685
                     /locus_tag="L2X49_000963"
                     /pseudo
     CDS             4942..5685
                     /locus_tag="L2X49_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(5742..7175)
                     /gene="bglA"
                     /locus_tag="L2X49_000964"
     CDS             complement(5742..7175)
                     /gene="bglA"
                     /locus_tag="L2X49_000964"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBN4796094.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            7220..7531
                     /locus_tag="L2X49_000965"
     CDS             7220..7531
                     /locus_tag="L2X49_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBN4796095.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYLIEFKC
                     L"
     gene            7695..8354
                     /locus_tag="L2X49_000966"
     CDS             7695..8354
                     /locus_tag="L2X49_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBN4796096.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8431..9411)
                     /gene="ygfZ"
                     /locus_tag="L2X49_000967"
     CDS             complement(8431..9411)
                     /gene="ygfZ"
                     /locus_tag="L2X49_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBN4796097.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9654..9920
                     /gene="sdhE"
                     /locus_tag="L2X49_000968"
     CDS             9654..9920
                     /gene="sdhE"
                     /locus_tag="L2X49_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBN4796098.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9901..10308
                     /locus_tag="L2X49_000969"
     CDS             9901..10308
                     /locus_tag="L2X49_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HBN4796099.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10348..10869)
                     /gene="fldB"
                     /locus_tag="L2X49_000970"
     CDS             complement(10348..10869)
                     /gene="fldB"
                     /locus_tag="L2X49_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBN4796100.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10981..11877
                     /gene="xerD"
                     /locus_tag="L2X49_000971"
     CDS             10981..11877
                     /gene="xerD"
                     /locus_tag="L2X49_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBN4796101.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11902..12612
                     /gene="dsbC"
                     /locus_tag="L2X49_000972"
     CDS             11902..12612
                     /gene="dsbC"
                     /locus_tag="L2X49_000972"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBN4796102.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12618..14351
                     /gene="recJ"
                     /locus_tag="L2X49_000973"
     CDS             12618..14351
                     /gene="recJ"
                     /locus_tag="L2X49_000973"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBN4796103.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14442..15540
                     /gene="prfB"
                     /locus_tag="L2X49_000974"
     CDS             join(14442..14516,14518..15540)
                     /gene="prfB"
                     /locus_tag="L2X49_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBN4796104.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQIKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15550..17067
                     /gene="lysS"
                     /locus_tag="L2X49_000975"
     CDS             15550..17067
                     /gene="lysS"
                     /locus_tag="L2X49_000975"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBN4796105.1"
                     /translation="MSEQHAQGADAVIDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17110..17658)
                     /gene="idi"
                     /locus_tag="L2X49_000976"
     CDS             complement(17110..17658)
                     /gene="idi"
                     /locus_tag="L2X49_000976"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBN4796106.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            17713..17784
                     /locus_tag="L2X49_000977"
     CDS             17713..17784
                     /locus_tag="L2X49_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796107.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKSI"
     gene            complement(17781..17906)
                     /locus_tag="L2X49_000978"
     CDS             complement(17781..17906)
                     /locus_tag="L2X49_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796108.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17908..19356)
                     /gene="uacT"
                     /locus_tag="L2X49_000979"
     CDS             complement(17908..19356)
                     /gene="uacT"
                     /locus_tag="L2X49_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBN4796109.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            19792..21711
                     /gene="ygfT"
                     /locus_tag="L2X49_000980"
     CDS             19792..21711
                     /gene="ygfT"
                     /locus_tag="L2X49_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HBN4796110.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     VAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVTIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSNL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21711..22199
                     /locus_tag="L2X49_000981"
     CDS             21711..22199
                     /locus_tag="L2X49_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBN4796111.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22235..23602)
                     /gene="ghxQ"
                     /locus_tag="L2X49_000982"
                     /gene_synonym="ygfQ"
     CDS             complement(22235..23602)
                     /gene="ghxQ"
                     /locus_tag="L2X49_000982"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HBN4796112.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(23638..24954)
                     /gene="guaD"
                     /locus_tag="L2X49_000983"
     CDS             complement(23638..24954)
                     /gene="guaD"
                     /locus_tag="L2X49_000983"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HBN4796113.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24972..26372)
                     /gene="xanQ"
                     /locus_tag="L2X49_000984"
     CDS             complement(24972..26372)
                     /gene="xanQ"
                     /locus_tag="L2X49_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HBN4796114.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(26537..29407)
                     /locus_tag="L2X49_000985"
     CDS             complement(26537..29407)
                     /locus_tag="L2X49_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HBN4796115.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(29404..30183)
                     /locus_tag="L2X49_000986"
     CDS             complement(29404..30183)
                     /locus_tag="L2X49_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HBN4796116.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLASPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(30234..31562)
                     /gene="ssnA"
                     /locus_tag="L2X49_000987"
     CDS             complement(30234..31562)
                     /gene="ssnA"
                     /locus_tag="L2X49_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBN4796117.1"
                     /translation="MLILKNVTAVQLHPAKVQEAVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFAAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDTLA"
     gene            complement(31565..34663)
                     /gene="ygfK"
                     /locus_tag="L2X49_000988"
     CDS             complement(31565..34663)
                     /gene="ygfK"
                     /locus_tag="L2X49_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HBN4796118.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVKKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(34985..35563)
                     /gene="mocA"
                     /locus_tag="L2X49_000989"
     CDS             complement(34985..35563)
                     /gene="mocA"
                     /locus_tag="L2X49_000989"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBN4796119.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            35666..36436
                     /gene="yqeC"
                     /locus_tag="L2X49_000990"
     CDS             35666..36436
                     /gene="yqeC"
                     /locus_tag="L2X49_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBN4796120.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSKC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            36484..38109
                     /locus_tag="L2X49_000991"
     CDS             36484..38109
                     /locus_tag="L2X49_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBN4796121.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFI
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(38150..39082)
                     /gene="arcC"
                     /locus_tag="L2X49_000992"
     CDS             complement(38150..39082)
                     /gene="arcC"
                     /locus_tag="L2X49_000992"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBN4796122.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(39130..40515)
                     /gene="hyuA"
                     /locus_tag="L2X49_000993"
     CDS             complement(39130..40515)
                     /gene="hyuA"
                     /locus_tag="L2X49_000993"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HBN4796123.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNIREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(40568..41779)
                     /locus_tag="L2X49_000994"
     CDS             complement(40568..41779)
                     /locus_tag="L2X49_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBN4796124.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(41837..43033)
                     /gene="dpaL"
                     /locus_tag="L2X49_000995"
     CDS             complement(41837..43033)
                     /gene="dpaL"
                     /locus_tag="L2X49_000995"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBN4796125.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(43091..44278)
                     /gene="ygeW"
                     /locus_tag="L2X49_000996"
     CDS             complement(43091..44278)
                     /gene="ygeW"
                     /locus_tag="L2X49_000996"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HBN4796126.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT        10818 a        11205 c        11786 g        10772 t
ORIGIN      
        1 aaccataagc taatgtgatg atcaatttta ccttatggtt aacagtctgt ttcggtggta
       61 agttcaggca aaagagaacg attgcgttgg ggaccgggag tggctccgat gctgggtttc
      121 gtggtgataa tttcactatg aaaaagttgt cagccccgct tattcaatga ggacaagatg
      181 gcacaacaga ctcctttgta cgaacaacat acgctttgcg gcgctcgcat ggtggatttc
      241 cacggctgga tgatgccgct gcattacggt tcgcaaatcg acgaacatca tgcggtacgt
      301 accgatgccg gaatgtttga tgtgtcacat atgaccatcg tcgatcttcg cggcagccgc
      361 acccgggagt ttctgcgtta tctgctggcg aacgatgtgg cgaagctcac caaaagcggc
      421 aaagcccttt actcggggat gttgaatgcc tctggcggtg tgatagatga tctcatcgtc
      481 tactacttta ctgaagattt cttccgcctc gttgttaact ccgccacccg cgaaaaagac
      541 ctctcctgga ttacccaaca cgctgaacct ttcggcatcg aaattaccgt tcgtgatgac
      601 ctttccatga ttgccgtaca agggccgaat gcgcaggcaa aagctgccac actgtttaat
      661 gatgctcagc gtcaggcggt ggaagggatg aaaccgttct ttggcgtgca ggcgggcgat
      721 ctgtttattg ccaccactgg ttataccggt gaagcgggct atgaaattgc gctgcccaat
      781 gaaaaagcgg cggatttctg gcgcgcgctg gtggaagcgg gcgttaagcc atgcggcctg
      841 ggcgcgcgtg acacgctgcg tctggaagcg ggcatgaatc tttatggtca ggagatggac
      901 gaaactattt ctcctttagc cgccaacatg ggctggacta tcgcctggga accggcagat
      961 cgtgacttca tcggtcgtga agccctggaa gtgcagcgtg agcatggcac agaaaaactg
     1021 gttggtctgg tgatgaccga aaaaggcgtg ctgcgtaatg aactgccggt acgttttacc
     1081 gatgcgcagg gcaaccagca tgaaggcatt atcaccagcg ggactttctc cccgacgctg
     1141 ggttacagca ttgcgctggc acgcgtgccg gaaggtattg gcgaaacggc gattgtgcaa
     1201 attcgcaacc gtgaaatgcc ggttaaagtg acgaaacctg tttttgtgcg taacggtaaa
     1261 gccgtcgcgt gatttacttt tttggagatt gattgatgag caacgtacca gcagaactga
     1321 aatacagcaa agaacacgaa tggctgcgta aagaagccga cggcacttac accgttggta
     1381 taaccgaaca tgcccaggag ctgttaggcg atatggtgtt tgttgacctg ccggaagtgg
     1441 gcgcaacggt tagcgcgggc gatgactgcg cggttgccga atcagtaaaa gcggcgtcag
     1501 acatttatgc gccagtaagc ggtgaaatcg tggcggtaaa cgacgcactg agcgattccc
     1561 cggaactggt gaacagcgaa ccgtatgcag gtggctggat ctttaaaatc aaagccagcg
     1621 atgaaagcga actggaatca ctgctggatg cgaccgcata cgaagcattg ttagaagacg
     1681 agtaacggct ttattcctct tctgcgggag aggatcaggg tgaggatatt tacgcctcac
     1741 cctcactctc ttcgcaagga gagaggttca caattcactg cacgtttcag gaaccatcgc
     1801 tcatgacaca gacgttaagc cagcttgaaa acagcggcgc ttttattgaa cgccatatcg
     1861 gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt tggtgcacaa tcgttaaacg
     1921 cgctgaccgg ccagattgtg ccgaaagata ttcagcttgc gaccccgccg caggttggcg
     1981 caccggcgac cgaatacgcc gcactggcag aactcaaggc tattgccagt cgcaataaac
     2041 gcttcacgtc ttacatcggc atgggttaca ccgccgtgca gctaccgccg gttatcctgc
     2101 gtaacatgct ggaaaatccg ggctggtata ccgcgtacac tccgtatcaa cctgaagtct
     2161 cccagggccg ccttgaagca ctgctcaact tccagcaggt aacgctggat ttgactggac
     2221 tggatatggc ctctgcttct cttctggacg aggccaccgc tgccgccgaa gcaatggcga
     2281 tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt cttcgtggct tccgatgtgc
     2341 atccgcaaac gctggatgtg gtccgtactc gtgccgaaac ctttggtttt gaagtgattg
     2401 tcgatgacgc gcaaaaagtg ctcgaccatc aggacgtctt cggcgtgctg ttacagcagg
     2461 taggcactac cggtgaaatt cacgactaca ctgcgcttat tagcgaactg aaatcacgca
     2521 aaattgtggt cagcgttgcc gccgatatta tggcgctggt gctgttaact gcgccgggta
     2581 aacagggcgc ggatattgtt tttggttcgg cgcaacgctt cggcgtgccg atgggctacg
     2641 gtggcccaca cgcggcattc tttgcggcga aagatgaata caaacgctca atgccgggcc
     2701 gtattatcgg tgtatcgaaa gatgcagctg gcaataccgc gctgcgcatg gcgatgcaga
     2761 ctcgcgagca acatatccgc cgtgagaaag cgaactccaa catttgtact tcccaggtac
     2821 tgctggcaaa catcgccagc ctgtatgccg tttatcacgg cccggttggc ctgaaacgta
     2881 tcgctaaccg cattcaccgt ttgaccgata tcctggcggc aggcctgcaa caaaaaggtc
     2941 tgaagctgcg ccatgcgcac tatttcgaca ctctgtgtgt ggaagtggcc gacaaggcgg
     3001 gcgtactggc gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatc ctgaacgcgg
     3061 ttgggatcac ccttgatgaa accaccacgc gtgaaaacgt aatgcagctt ttcagcgtgc
     3121 tgctgggcga taaccacggg ctggagatcg acacgctgga caaagacgtg gctcacgaca
     3181 gccgctctat ccagcctgcg atgctgcgcg acgacgaaat cctcacccat ccggtgttta
     3241 atcgctacca cagcgaaacc gaaatgatgc gctatatgca ctcgctggag cgtaaagatc
     3301 tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac catgaaactg aacgccgccg
     3361 ccgagatgat cccaatcacc tggccggaat ttgccgaact gcacccgttt tgtccgccgg
     3421 aacaggctga aggttatcag cagatgattg cgcagctggc tgactggctg gtgaaactga
     3481 ccggttacga cgccgtttgt atgcagccga actccggcgc acagggcgaa tacgcgggcc
     3541 tgctggcgat tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat atctgcctga
     3601 tcccggcttc tgcgcacgga actaaccccg cttctgcaca tatggcagga atgcaggtgg
     3661 tggttgtggc gtgtgataaa aacggcaaca tcgatctgac tgatctgcgc gcgaaagcgg
     3721 aacaggcggg cgataacctc tcctgtatca tggtgactta tccttctacc cacggcgtgt
     3781 atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt caggtttacc
     3841 ttgatggcgc gaacatgaac gcccaggttg gcatcacctc gccgggcttt attggcgcgg
     3901 acgtttcgca cctcaacctg cataaaactt tctgcattcc gcacggcggt ggtggtccgg
     3961 gtatgggacc gatcggtgtg aaagcgcatc tggcaccgtt tgtaccgggt catagcgtgg
     4021 tgcaaatcga aggcatgtta acccgtcagg gcgcagtttc tgcggcaccg ttcggtagcg
     4081 cctctatcct gccaatcagc tggatgtaca tccgcatgat gggcgcagaa gggctgaaaa
     4141 aagcaagcca ggtggcaatc ctcaacgcca actatattgc cagccgcctg caggatgcct
     4201 tcccggtgct gtataccggt cgcgacggtc gcgtggcgca cgaatgtatt ctcgatattc
     4261 gcccgctgaa agaagaaacc ggcatcagcg agctggatat tgccaagcgc ctgatcgact
     4321 acggtttcca cgcgccgacg atgtcgttcc cggtggcggg tacgctgatg gttgaaccga
     4381 ctgaatctga aagcaaagtg gaactggatc gctttatcga cgcgatgctg gctatccgcg
     4441 cagaaatcga ccaggttaaa gccggtgtct ggccgctgga agataacccg ctggtgaacg
     4501 cgccgcacat tcagaacgaa ctggtcgccg agtgggcgca tccgtacagc cgtgaagttg
     4561 cagtattccc ggcaggtgtg gcagacaaat actggccgac ggtgaaacgt ctggatgatg
     4621 tttacggcga ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac cagtaattca
     4681 ctgattcgac tatcttctaa aggcgcttcg gcgccttttt agtcagatga caaagtacaa
     4741 aagcgctcag acagtcccct cacccctttg gggagagggt tagggtgagg ggaacaggcc
     4801 ggcactggcg cgaatattta ccctcacccc ggccctctcc ctgaaagggc gaggggaaaa
     4861 gcgtgccaac attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat
     4921 taatttttct cggaaaaaca aatggctata gcacttgtga ccggtggcag tcgcggcatc
     4981 gggcgggcaa ctgcattact gttggcgaaa gaagagtata cggtggcggt taattatcaa
     5041 caaaacctcc atgcggcgca gtaagtggtg aacttaatca cgcaagccgg tggcaaggca
     5101 ttcgtgctcc aggcggatat cagcgacgaa aatcaggtca tcgcaatgtt tacagcaatc
     5161 gatcagcacg atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag
     5221 tgcaccgttg aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg
     5281 ggatattttc tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt
     5341 ggcggcgcta tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat
     5401 gttgattacg cggcatcgaa aggggcgatt gatacgttaa ccaccggact atcgctggaa
     5461 gtcgccgcgc aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg
     5521 cacgccagcg gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag
     5581 cgtggtggac aggcagaaga ggtcgcgcag gccattgtct ggctactaag tgataaagcc
     5641 tcttacgtca cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc
     5701 tgaccggatg caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg
     5761 ctggcaatca cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca
     5821 gtaccgtcgt catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta
     5881 gtgaatgaca cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacaccatcg
     5941 taagtcactg cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtagtcg
     6001 tcgttgatgc tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata
     6061 aacagcggcc tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga
     6121 tcaatctgcc agccccagtc agacgcttta acatacgggt tcggtacgct gccttcaaaa
     6181 ccagagatcg catcgcctgt gccgccttcg gccttcactg cgttggtcat gtaatagctg
     6241 aaaccaagat aatcgcaggt gccttcacgc agtacatcga gatcgccgtc ttccattttg
     6301 atgttaaatc cgcgacgctc ccactcgttc aacacatagg acgggtaata gccgcgtagc
     6361 tgcacatcgg taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc
     6421 gggttacagc agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc
     6481 gggttaatgc gacgcgcagc tttcaccgcc agggcgctgg cgacaaactg gtgatgcagc
     6541 acctgataca tcgtctcttc cgggttctca tgctcggtat aaaccacgcc ggagcagcag
     6601 taaccgaaca gcggtgcacg ccagttacgc tggttgttaa tttcgttgaa ggtcatccag
     6661 tatttgactt tatgcttata gcgttcaaac accacttccg cgaaacggac aaagaaatca
     6721 accactttac ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag
     6781 tgggagaggg tgatcaccgg ttcgatgttg tatttcagca gttcatcgaa catatcatcg
     6841 tagaacttca gcccttcttc gtttggctga gcttcatcac cttttggaaa aatacgcgtc
     6901 caggcgatgg aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc
     6961 ttgtagtgac cataaaaatc aacggcttca tggtttggat agtatttgcc tggcaagact
     7021 tctttggtga tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc
     7081 ggccctttgc cgcctttgtt ccagccgcct tcgacctgat gagcggcaac tgcgccgccc
     7141 cataagaaat ctttcggtaa ggtgagtttt ttcactatca taggctccag atgagtcaat
     7201 tcttacagga aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga
     7261 tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa
     7321 aacgggtgat gtgcttcgtg tcgggcgttt tgaagatgac ggttattttt gcacgattga
     7381 agtcaccgca acctcaaccg taacgctgga tacgctgacg gaaaaacatg cagagcagga
     7441 aaatatgacc ctgactgaac tgataaaggt cattgccgac atctatcccg gtcagacaca
     7501 gttttatttg attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt
     7561 cgcatttaat ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac
     7621 aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt
     7681 ctccggagta agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg
     7741 aaattgccaa cagcgtcagt cacggcattg ggttggtgtt tggtatcgtt gggctggtgt
     7801 tgctactggt tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc
     7861 tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc
     7921 atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg
     7981 ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga
     8041 tgattgttat ctggagcctg gcattgctgg gtattctgtt taaactgacc atcgcgcacc
     8101 gattcaaaat tttatctctg gtgacctatc tggcgatggg ctggctgtcg ctggtggtaa
     8161 tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg
     8221 tggtttattc gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca
     8281 tctggcacgg cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata
     8341 ttgggcaggc gtaatgggta tgcctttgta ggcctgataa gacgcgccag cgtcgcatca
     8401 ggcgataaat gcctgatgcg ataaagagat ttactcttcg agcgaatacg gcagcggctc
     8461 gatatgcagt gtattcgcat cgtcgcgtac gcggaagatg ctatccggtt ccatatcgtt
     8521 attcatgacg acctgtacca cgacctgacc atcttccagt tttaccgcag ccagcaccgt
     8581 accggtacga cgccagttct cgcccatttt cagctctaag tcttcaccag cttccggcag
     8641 tcggctggcg ctacctgcca gcaaccagag cgcacgttta ttggcaccac ggaattttgc
     8701 tcgcgccacc atctcttgtc cggtatagca gcctttttta aagctgatac cgcccagcgc
     8761 ctggaggttg gtcgcctgtg ggataaactg cccgctgttg gcggcatcaa tcaccgggaa
     8821 acccgcttca atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg
     8881 cagtttatcg gtcagcatat tagcagtagc ttcatcggtt acgatcagga aacgttctgc
     8941 cgggtgttca aaccatagca aagtggtcgc gccttcttta actacctgtt tttctttcga
     9001 aggcagttca ctaaagagat ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag
     9061 cagcacacgc tcgtcgtctg gcgcgatggt cactttagag aataccgcat attttttcag
     9121 ttcagtcagc tgcggttcac gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc
     9181 gcggaacaga cgtaaattgc tccacatttt acctttggcg tcgcaatggg cggcgagcag
     9241 gtgctgatct tctgccatct ggctgacatc tgctgtcacc tgaccctgca tatatttttc
     9301 gctgtccgcg ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg
     9361 taaacgggca gaagccgtag gctgacgggg aggaaaaggt gtaaaagcca taatagagtc
     9421 ctgagttgct taacggcgta ttaatgacta atggtaaaag agctatgagg caatgcaagt
     9481 gctttcacta cgcgatctgt gccttctgcg aggcgacttc caaaaaaatg aagccattga
     9541 attgttgtga attaatttcg cgatcgcgat ggtgtttact gatattcccg taaaagaagg
     9601 cgaaaaacac gttacaatag cggggtactg gattttcgtg agacaggaag aagatggaca
     9661 ttaacaacaa agcccgcatt cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt
     9721 caatcatgcc gtttttcgaa catgagtacg acagcttaag cgatgacgaa aaacgcatct
     9781 ttattcgtct gctggaatgt gacgatccgg acctgtttaa ctggctgatg aatcacggta
     9841 aaccggccga tgcagaactg gaaatgatgg tccgactcat ccagacacgg aaccgggaac
     9901 gtggtcctgt ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg
     9961 attcatgggc tggttgccgc tgttatttta ctcatgccct ggccactcag ttacaccccg
    10021 ttatggatgg tgttactttc gctggtggtg tttgattgcg ttcgcagcca gcggcgtatt
    10081 aatgctcgcc agggggaaat tcgcttgttg atggacgggc gtttgcgttg gcaagggcag
    10141 gagtggagca tcgtcaaagc accgtggatg attaagagcg gcatgatgct gcgtttacgt
    10201 tctgatggcg gtaaacggca acatttatgg ctggcagccg acagcatgga cgaagccgaa
    10261 tggcgggatt tacggcggat tttgttgcaa caagagacgc aaagataaag atgacggcaa
    10321 agcatatgcg ctaagccgtc agtgagatca ggcgtaatgc tctgccattt cgttgaggat
    10381 ttgctcgcac cagctctgaa tacgctcgtc gctaaggtca tactggttag tttcatccag
    10441 cgccagaccc acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg taaattcata
    10501 tccttccgtt ggccagtagc cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag
    10561 cataccgagc gcatcgagga accactcgcc gtatcccagt tgatcgccaa gcccatacag
    10621 cgcaacaatt ttaccttcaa ggttcaggtc gtcgagctga tcccagacgg cttcccagtc
    10681 ttcttggatt tcaccaaaat cccaggtcgg gatacccaga atgagcacat cgtactgctc
    10741 cattaatttc ggggagtcgt ccttgaggtt atgtaaggtc accagttctg ggccgataat
    10801 atcgcggatt ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc
    10861 catattcata acgtaagctt ctcgattctg ctgtggcttt tgcggctagt gtatcagaat
    10921 cgctttagtc acggcacaat aaggcataat ggatcaaatg agtgaccata aggggcgcaa
    10981 gtgaaacagg atctggcacg catcgagcag tttcttgatg ctctgtggct ggaaaaaaat
    11041 ctggctgaaa atacgttgaa cgcttaccgt cgcgatctgt caatgatggt ggagtggttg
    11101 catcaccgcg ggttgacgct ggcgacggcg caaagtgacg atttgcaggc attactggca
    11161 gaacggctgg agggcgggta taaagccacc agctcagcgc gtttgctgag tgcagtgcgc
    11221 cgattgttcc agtatcttta tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc
    11281 gcttcaccga aattgcccca gcgtttgcca aaagatttaa gcgaagcgca ggttgaacgt
    11341 ttattacagg caccattaat tgatcagcca ctggagctac gcgataaagc catgcttgaa
    11401 gtgttgtatg ctaccgggct gcgtgtctct gaactggtcg gactgacaat gagtgatatc
    11461 agcctgcgtc agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg cctggtaccg
    11521 ttaggtgaag aggcggttta ctggctggaa acctatctgg aacatgggcg tccgtggctg
    11581 ctgaatggtg tgtcaattga cgtgttgttt cccagccagc gtgcgcagca gatgacgcga
    11641 cagaccttct ggcaccgcat taaacattat gctgtgctgg cgggtatcga cagcgaaaag
    11701 ctgtcaccgc atgtgttgcg tcacgctttt gccacccatt tattaaatca tggtgcggat
    11761 ttacgcgtgg tgcagatgct actgggccac agcgacctct ccaccacgca aatttatacg
    11821 catgtcgcta ccgagcgtct gcggcaactt catcaacagc atcacccgcg ggcgtgatgt
    11881 ctgaaaagaa cgggaagatt tatgaagaaa ggttttatgt tgtttacttt gttagcggcg
    11941 ttttcaggtt ttgctcaggc tgatgacgcg gcaattcaac aaacgttagc caaaatgggc
    12001 atcaaaagca gcgatattca gcccgcgccc gtagctggca tgaagacagt tctgactaac
    12061 agcggcgtgt tgtacatcac cgatgatggt aaacatatca ttcaggggcc aatgtatgac
    12121 gttagcggca cggctccggt caatgtcacc aataagatgc tgttaaagca gttgaatgcg
    12181 cttgaaaaag agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt
    12241 actgatatta cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg
    12301 ctggggatca ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag cgatgcagag
    12361 aaagaaatga aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg
    12421 gcaggtaaaa gcgtcgcacc agccagctgc gacgtggata ttgccgacca ttacgcactt
    12481 ggcgtccagc ttggcgttag cggtactccg gcagttgtgc tgagcaatgg cacacttgtt
    12541 ccgggttacc agccgccgaa agagatgaaa gaattcctcg acgaacacca aaaaatgacc
    12601 agcggtaaat aattcgcgtg aaacaacaga tacaacttcg tcgccgtgaa gtcgatgaaa
    12661 cggcagactt gcccgctgaa ttgcctccct tgctgcgccg tttatacgcc agccggggcg
    12721 tgcgcagtgc gcaagaactg gaacgcagtg ttaaaggtat gttgccctgg cagcaactga
    12781 gcggcgtcga aaaggccgtt gagatccttt acaacgcttt tcgcgaagga acgcggatta
    12841 ttgtggtcgg cgattttgac gccgacggcg cgaccagcac ggctctaagc gtgctggcga
    12901 tgcgctcgct tggttgcagc aatatcgact atctggtacc aaaccgtttc gaagacggtt
    12961 acggcttaag cccggaagta gtcgatcagg cccatgcccg tggcgcgcag ttaattgtca
    13021 cggtggataa cggtatttcc tcccatgcgg gcgttgaaca cgctcgctcg ttgggcattc
    13081 cggttattgt taccgatcac catttgccgg gcgaaacatt acccgcagcg gaagcgatca
    13141 ttaaccctaa cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg
    13201 cgttttatct gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc
    13261 gtggcatcgc aattcctaac ctggcagaac tgctggatct ggtagcgctg gggacagtag
    13321 cggacgtcgt gccgctggac gctaataatc gcattctgac ctggcagggg atgagtcgca
    13381 tccgtgccgg aaagtgccgt ccggggatta aagcgctgct ggaagtggca aatcgcgatg
    13441 cgcaaaaact cgccgccagc gatttaggtt ttgcgctggg gccacgtctc aatgctgccg
    13501 gacgactgga cgatatgtcc gtcggtgtgg cgctcttgct gtgcgacaac atcggcgaag
    13561 cgcgcgtgct ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag
    13621 gaatgcaagt tgaagccctg accctgtgcg agaaactgga gcgaagtcgc gacacgctac
    13681 ccggcgggct ggcaatgtat caccccgaat ggcatcaggg cgttgtcggt attctggctt
    13741 cgcgcatcaa agagcgtttt caccgtccgg ttatcgcctt tgcgccagca ggtgatggta
    13801 cgctgaaagg ttcaggtcgc tccattcagg ggctgcatat gcgtgatgca ctggagcgat
    13861 tagacacact ctaccctggc atgatactga agtttggcgg tcatgcgatg gcggcgggtt
    13921 tgtcgctgga agaggataaa ttcgaactct ttcaacaacg gtttggcgaa ctggttacag
    13981 agtggctgga cccttcgcta ttgcaaggcg aagtggtgtc agacggtccg ttaagcccgg
    14041 ccgaaatgac catggaagtg gcgcagttgc tgcgcgatgc tggcccgtgg gggcagatgt
    14101 tcccggagcc gctgtttgac ggtcatttcc gtctgctgca acagcggctg gtgggcgaac
    14161 gtcatttgaa ggtgatggtc gaaccggtcg gcggcggtcc actgctggat ggtattgctt
    14221 ttaatgtcga taccgccctc tggccggata acggcgtgcg tgaagtgcaa ctggcttata
    14281 agctcgatat caacgagttt cgcggcaacc gcagcctgca aattatcatc gacaatatct
    14341 ggccaattta gcgtcatctt ctctataaaa aagagcgtgg attgggtaca atcccgctct
    14401 tatcaccgca ttttgactag ctcaataaaa gaaatcagac catgtttgaa attaatccgg
    14461 taaataatcg cattcaggac ctcacggaac gctccgacgt tcttaggggg tatctttgac
    14521 tacgacgcca agaaagagcg tctggaagaa gtaaacgccg agctggaaca gccggatgtc
    14581 tggaacgaac ccgaacgcgc acaggcgctg ggtaaagagc gttcctccct cgaagccgtt
    14641 gtcgacaccc tcgaccaaat caaacagggg ctggaagatg tttctggtct gctggaactg
    14701 gctgtagaag ctgacgacga agaaaccttt aacgaagccg ttgctgaact cgacgccctg
    14761 gaagaaaaac tggcgcagct tgagttccgc cgtatgttct ctggcgaata tgacagcgcc
    14821 gactgctacc tcgatattca ggcggggtct ggcggtacgg aagcacagga ctgggcgagc
    14881 atgcttgagc gtatgtatct gcgctgggct gaatcgcgtg gtttcaaaac tgaaatcatc
    14941 gaagagtcgg aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat
    15001 tacgcttacg gctggctgcg tacagaaact ggcgttcacc gcctggtgcg taagagcccg
    15061 tttgactccg gcggtcgtcg ccacacatcg ttcagctctg cgtttgttta cccggaagtt
    15121 gatgatgata ttgatatcga aatcaacccg gcggatttgc gcattgacgt ttatcgcgcg
    15181 tccggcgcgg gcggtcagca cgttaaccgt accgaatctg cggtgcgtat tacccacatc
    15241 ccaaccggga tcgtgaccca atgccagaac gaccgttccc agcacaagaa caaagaccag
    15301 gccatgaagc agatgaaagc gaagctttat gaactggaga tgcagaagaa aaatgccgag
    15361 aaacaggcga tggaagataa caaatccgac atcggctggg gcagccagat tcgttcttat
    15421 gtccttgatg actcccgcat taaagatctg cgtaccgggg tagaaacccg caacacgcag
    15481 gccgtgctgg acggcagcct ggatcaattt atcgaagcaa gtttgaaagc agggttatga
    15541 ggaaccaaca tgtctgaaca acacgcacag ggcgctgacg cggtaatcga tcttaacaat
    15601 gaactgaaaa cgcgtcgtga gaagctggcg aacctgcgcg agcaggggat tgccttcccg
    15661 aacgatttcc gtcgcgatca tacctctgac caattgcacg cagaattcga cggtaaagag
    15721 aacgaagaac tggaagcgct gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc
    15781 cgtattatgg gtaaagcgtc tttcgttacc ctgcaggatg tgggtggccg cattcagctg
    15841 tacgttgccc gtgacgatct gccggaaggc gtttacaacg agcagttcaa aaaatgggac
    15901 ctcggtgaca tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct
    15961 atccactgta ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc ggataaattc
    16021 cacggcttgc aggatcagga agcgcgctat cgtcagcgtt atctggatct catctctaac
    16081 gatgaatccc gtaacacctt taaagtgcgc tcgcagatcc tctctggtat tcgccagttc
    16141 atggtgaacc gcggctttat ggaagttgaa acgccgatga tgcaggtgat ccctggcggt
    16201 gccgctgcgc gtccgtttat cacccaccat aacgcgctgg atctcgacat gtacctgcgt
    16261 atcgcgccgg aactgtacct caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa
    16321 atcaaccgta acttccgtaa cgaaggtatc tccgtacgtc ataacccaga gttcaccatg
    16381 atggaactct acatggctta cgcagattac aaagatctga tcgagctgac cgaatcgctg
    16441 ttccgtactc tggcacagga tattctcggt aagacggaag tgacctatgg cgacgtgacg
    16501 ctggacttcg gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc
    16561 ccggaaaccg acatggcgga tctggataac ttcgactctg cgaaagcgat tgctgaatct
    16621 atcggtatcc acgttgagaa gagctggggt ctgggccgta tcgttaccga gatcttcgaa
    16681 gaagtggcag aagcacatct gattcagccg accttcatta ctgaatatcc ggcagaagtt
    16741 tctccgctgg cgcgtcgtaa cgacattaac ccggaaatca cagaccgctt tgagttcttc
    16801 atcggtggtc gtgaaatcgg taacggcttt agcgagctga atgacgcgga agatcaggcg
    16861 caacgcttcc tggatcaggt tgccgcgaaa gacgcaggtg acgacgaagc gatgttctac
    16921 gatgaagatt acgttaccgc actggaacat ggcttaccgc cgacagcagg tctggggatt
    16981 ggcatcgacc gtatggtaat gctgttcacc aacagccata ccatccgcga cgttattctg
    17041 ttcccggcga tgcgtccggt gaaataagca ttacgttatg ctcacaaccc cggcaaatgt
    17101 cggggttttt tatttaagct gggtaaatgc agataatcgt attctggctt cgcgatttgt
    17161 cgcctgcatc accatccacg gactgaacgc ccacggcgtg gcatcaatac cgtgtaatac
    17221 atcagctaaa tcacaccatt gataatccat cacttcatca tcgttgatct gtaacgcact
    17281 ggtggtgcgt gcggcaaata ccggacacac ttcattttcc acaatgccac tcggatcggt
    17341 ggcgcggtag cgaaagtcag gatagataga ttcaggaggc gtaatttcca cgccaagctc
    17401 ataacggcaa cggcggatca ccgcgtcttc gctgctttct cccagttgtg ggtgcccaca
    17461 aaccgagtta gtccacacgc caggccacgc ttttttgctc agtgcgcggc gggtaactaa
    17521 taattgcccc ttggcattaa acagccaact ggagaacgcg agatgtaaga gggtgtctgc
    17581 cgtgtgtgcg gcatactttt ccagcgtacc agtgggaact ccctgtgcat tcaataaaat
    17641 gacgtgttcc gtttgcataa tttctcacat gtaattctga tcactcatca ttatagataa
    17701 ttgaagagtg aaatgattaa ccaagtgagc gtttatcgac aaccgcccgt tttgagcgga
    17761 tgccgacagg taaaatccat ttaaaaaagt agcatcatat atttgaagcg actttcaatg
    17821 gccattgcaa cacagtcaga aatgacagga atagagagag taaaaagcaa cagcagactg
    17881 aatatagcgc gcattaaaaa attcatatta ttctccatgc tcatttttct tcagcttgcg
    17941 catcagaagc cacatacgta ctgtcctgac ttttaaatct ttatcagaga cggattcctt
    18001 caccaggtca gcatgatgct ggtagccatt aaagaagaca ttcagcacaa cggcgctaag
    18061 ggttgcgagc ataatgccgc tatgcagcaa cggttgcagt acggccggta acttagaaaa
    18121 gaaatcgtga gagagcgtcg gagtcatgcc aacgccgaga ctgatcgcca caatatagag
    18181 gttgtaacgg ttggtggtgt agttacagcg cgacagaatt cgaatccctg tcgccagtac
    18241 catgccgaac atcaccagcc cagcaccgcc cagcacaaat tgcggaatgg aggcgaccag
    18301 caccgccatt tttggcacca tgccaaacag gattaatata attcccgaag aaatacacac
    18361 ccagcggcta tgaacgcgcg tcacgctaac caggccaacg ttttgtgaaa atgacgtgtg
    18421 ggggaagctg ttaaacgttc cgcctatcat tgtccctacg ccatcgacac gcagcccgcg
    18481 aataatatcg tgtgaagaga gtttacgacc gactatttca cccagtgcca ggaacatccc
    18541 catcgactcg ataaacacga tgattaacac ggcagtcatg gtcagaatgg aaacgggatc
    18601 gaaaatcggc ataccaaatg acatcggcgt gacaatcgca aaccatgaag catcatgtag
    18661 cccggataaa ttgacttcat tcatcatcca cgaaagtaaa aagccaaata caatccccag
    18721 taatacggcg acgttggaca taaatccttt cgcatagcga gtaatgagca agataaaaat
    18781 taagacagca aaggagatac ctaaataaac gggattacca tattgcggat tccctttacc
    18841 tccggcggcc cagtcaatac ccacctgaat gatgctaagc ccaatagaag taataaccac
    18901 accggtaacc agtggcggga ataaaggcat caagcgaccg ataagtggcg ctaataatgt
    18961 ggtgataaaa cccgcggcga tagtggcacc aaatatcccc agcaggccga tatccgggtt
    19021 catcccaatg gctatcattg gtgttacagc agcaaaggtc accgacataa tcaccggcag
    19081 gcggatcccc ataaagcggc cgataccgat acattgcaat aatgtgacga tcccgcagca
    19141 aaagagatcc gagctaatga gcatcgcaat agcatctttt gagaggccca gtcggtcacc
    19201 aatcataaga ggaacagcga ctgcacctgc gtacatgaca aggacgtgtt gcagaccgag
    19261 tatgatcagc tttcctggtg ataatatgcg gtcaacctca tcagttgggc ggtcttgccc
    19321 agaagatgag ggaagttggg aatctatggc gctcatcagt gtttctcctt tcgcccgcca
    19381 gagtaaaaag caaccgttta cgttaatgaa actgtctaaa cacatgctcg tggagtgcca
    19441 gtttttgcga ggaaggggag ttagagacag gaattgcgga ggcaatcaca taatcgtgct
    19501 taaaccacga aaatgacaag agaacctgac aatggaatta tctgtaattt tctcatattg
    19561 atatttcatt aatcattctg gcatgtcgca ctctcgcatt taatcgtttt tatctggata
    19621 gcgctctttt ggtcggcaat gttgaaataa tgaaagcggc atagattgag gggcaataat
    19681 ttgaactcag tcgcaaaatt ataattaaaa cggtcatgct acctaatata tgcccgttta
    19741 ttgcatcgcg aaatttgaga agacataact cattaaatga aagggatgca aatgaataag
    19801 tttatcgctg ctgaagctgc ggaatgtata ggctgtcatg cttgtgaaat tgcctgtgcg
    19861 gtggcacaca atcaagaaaa ctggccgctg agtcacagtg actttcgacc gcgtatccac
    19921 gttgtaggga aaggccaggc tgcgaatccg gtggcctgcc atcactgcaa caatgcccct
    19981 tgcgttacgg cttgtccggt taatgctctg actttccagt ccgatagcgt acaactggac
    20041 gagcaaaaat gtattggctg taaaagatgc gcaatcgctt gcccctttgg cgtcgttgag
    20101 atggtcgata cgattgcgca gaaatgcgac ctttgtaacc agcgcagttc tggcacgcaa
    20161 gcctgtattg aagtctgccc aacacaggcg ttacgactga tggacgataa agggttacag
    20221 cagataaagg tggcccgcca gcgcaaaacg gcagcaggaa aagcgtcatc agtcgctcag
    20281 ccatctcgca gtgcagcgtt gctccccgtt aactcgcgta aaggcgcaga taaaatttca
    20341 gcaagtgaac ggaaaaacca ctttggcgaa atctattgcg ggctggatcc actgcaagcg
    20401 acttatgaga gtgaccgctg tgtttattgt gccgaaaaag ctaactgcaa ctggcattgc
    20461 ccgctgcata acgctattcc ggattacatc cgtctggtac aggagggaaa gattattgaa
    20521 gcggcagaac tttgccacca gaccagttcc ttaccagaaa tctgcggcag ggtatgtcca
    20581 caggaccgtc tttgtgaagg cgcatgtact ttgaaagatc actctggcgc agtcactatc
    20641 ggtaatctgg aacgctacat caccgatacc gcgctggcga tgggctggcg tcccgatgtc
    20701 agcaaagttg ttccccgtag cgaaaaagtg gcggtgattg gcgctgggcc agcagggtta
    20761 gggtgtgctg atattctggc gcgcgcagga gttcaggttg atgtctttga tcgccatcca
    20821 gaaattggcg gtatgctgac ttttggcatt cctcctttca aactcgataa aacggtatta
    20881 agccagcggc gagagatatt caccgcaatg ggaatcgact ttcatctcaa ctgtgaaatt
    20941 ggccgcgata ttacctttag caatttaact tctgaatatg atgcagtttt catcggcgtg
    21001 gggacttacg ggatgatgcg agcagatctg ccgcatgaag atgcgcccgg tgtcattcag
    21061 gctctaccgt tcctgactgc ccatacccgc cagctcatgg gattgccgga gtctgaagag
    21121 tatccgctga cggacgtgga aggtaagcga gtcgtggtat tgggcggtgg cgatacgaca
    21181 atggattgtt tgcggacttc catccgcctc aatgccgcca gcgtgacctg cgcgtatcgt
    21241 cgtgatgaag tcagtatgcc gggctcgcgc aaagaggtgg ttaatgcgcg cgaggaaggt
    21301 gtcgagtttc agttcaatgt tcaaccgcaa tatatcgctt gtgacgaaga tgggcgttta
    21361 actgcggtgg gcctgattcg taccgccatg ggtgagccgg ggccggatgg tcgccgtcgt
    21421 cctcgtccgg tagcgggttc agagtttgaa ttgcccgccg atgttctgat tatggccttt
    21481 ggtttccagg cgcatgccat gccgtggttg cagggcagcg gaattaaact cgataaatgg
    21541 ggcctgattc aaaccggtga cgtcgggtat ttacctaccc agacgcatct gaaaaaagtc
    21601 tttgctggtg gtgatgcagt tcatggcgcg gatctggttg tcactgcaat ggccgcagga
    21661 aggcaggcgg cgcgcgatat gttaactctg tttgatacga aggcatcgtg atgaaatcgt
    21721 taattatcgt taatccggct gactgtattg gctgccgtac ttgtgaagta gcctgtgtgg
    21781 tcgctcaccc ttcagaacag gagttgaatg ccgatgtctt tttaccccgg ctgaaggtac
    21841 agcgactgga cagcatcagt gcgccggtga tgtgccatca gtgtgaaaac gccccttgtg
    21901 ttggcgcttg ccccgtgggg gcgctgacga tgggcgagca ggtggtgcag accaattctg
    21961 cccgttgtat tggctgtcag agttgcgtca gtgcgtgccc gtttggaatg atcaccattc
    22021 agtcgttgcc gggggatacc cgacaacaaa ttgtgaaatg cgatctctgt gaacagcggg
    22081 aagaggggcc agcctgtgtt gaatcttgcc cgacacaggc gctgcagttg cttactgaaa
    22141 gagaactcag gcgagtccgc cagcagcgta ttgttgccag cggtgagaat cctctgtgaa
    22201 agaaatctcc gcgctgaaag gcgcggagaa tctattagat tgcccaacca cccgcgtaaa
    22261 atgcgaccag tgcggcagta atgatcaccg taccaatatt cagcttttgc cattcgcgtg
    22321 caaagacgcg gcctacgacc agtgtcacaa agcccagcat aataccggta acgatattac
    22381 aagtcagaac gatgaacacg gcacacacca ggccagccat agcgtcaata aaatcattga
    22441 aatccagctt cgagacgtta cttaacatca gcaaacccac gtacatcaga gcgggtgcag
    22501 tggcgtaacc agggatcaaa aatgacagcg gtgataagaa cagaatcagc aggaataacg
    22561 cccccactac ggttgcggtc aaccctgttt tacccccggc ggcggttcct gccgctgatt
    22621 cgatataaac cgctgcgggc gctgcgccca ccagaccgga gaatattgaa cttactgagt
    22681 cactggtcag ggctttgccg ccgttgatga tctggttgtc tttatccaac aaattcgcct
    22741 gaccggcgac ggcacggatg gtgccagtgg cgtcgaacac tgcggtcatc accaatgcca
    22801 gtacactcgg aagtacagtt ggctggagtg cgcccataat atcgaggctg aaaatcagag
    22861 acttaccatc ttcgccagtc aggcttggca tcgccaccag accgtggtat ttcaccgctg
    22921 ggtcaaagat taagccgatg atcgaaattg caataatcac caacaagatc ccgccgggta
    22981 cgcgacactt ctccagaccg aagatgaccg ccagccccag taagctcatc atcaccggga
    23041 aggaggtaaa cgcaccgagc gccactggca agccttcaat cgggtttttg ataaccattc
    23101 ccacaccgtt agcagcaatc agcagcagaa acaggccgat accgatacct gtaccgtgag
    23161 cgatccccat cggtaaatta cgtaagatcc aggtacgcac accggttacg gaaatagcgg
    23221 tgaagatgac gcccatcaga aagaccgcgc ccagtgcgac aggaacgcta atttgttgcc
    23281 cgagtaccag actgaatgcg gtaaacgccg tcaaggaaat cgcgcaacca atcgccattg
    23341 gcaaattggc ccataatccc atcagcaacg agccgaagcc cgcgaccaga caggtggcaa
    23401 caaacacagc tgcgggagga aaacctgctt tgcccagcat tcccggaacg acgataacgg
    23461 aataaaccat ggccagaaag gtcgttaagc cagccagtac ttcctgacga acggtactgc
    23521 cacgagcggt aattttaaaa taattatcca gcgcgccctg tggctttggt gcgtccggtg
    23581 tttgtaggat gtctccagac ataatgatgt cctcacgagg atgttaaagt tttattatta
    23641 gttgcgttcg tacaccagac gaccatcaac gtaggtgcgg tagatcgaac ggtcatcgcc
    23701 caacgtcatc atcacgaaca atttgtcgac taaagaaaca gagttgtcat agcgcagctg
    23761 ctgtagcgga gtggcggtgg gttccatcac cacgaaatca gcctctttgc caggtaaaaa
    23821 gttgccaatc aaatcgtcaa ggcccagaga tttcgctccg ccgagcgtgg ccaggtaaaa
    23881 cgcttcatat gccgagaggc gatagccttg taattgcaac actttgtagg cttcgttcag
    23941 cgtttgcagc atgttgaaag tggttccggc accgatatcc gttcccatgc ccactttaac
    24001 tttcttctgc catgcttttt tcaagttgaa taagccgctg ccgaggtaaa ggttggaggt
    24061 cggacagaaa gcaatgctgg atttggtttc gctgagacga tcccactctt tttcttcgag
    24121 atggacgcag tgagcaaaga cacagttttt accggtcagg ccgtactgat ggtaaacatc
    24181 cagataacca tcatggtcag gataaagcga tttcacccag gcaatttcat ctttgttttc
    24241 acagagatgg gtatgtaccc acgtatccgg atattcttct ttcaggcgtt gcgccatcgc
    24301 catctgttca ggagatgagg tcggggcgaa gcgtggcgta atcgcatata gcagacgacc
    24361 atttttgtgc cagcgttcaa tcagttcttt gctttggtga tagctgcttt cggcagtgtc
    24421 gagcagataa tccggtgcgt tacggtccat catcacctta ccggcaatca tacgcatatt
    24481 gatatgactg gcggcttcaa acagcgcatc aacggattgc ggatgaacag tgccaaacac
    24541 cagcgccgtg gtggttccgt tacgtaaaag ctgcttgatg aagaacgccg acatttcgcg
    24601 ggcgtactct aaatcctcat aacgccgttc agtagggaag gtgtgtttat tcaaccactc
    24661 cagcaattgc tcaccatagg cccccaccat ttcactttgc ggataatgga tatgtgtatc
    24721 gacaaagccc ggtactatca gtttgccgcg atagtcgcgc acgcgaatgg tgtcaggaat
    24781 ttgatgcttt ccgttttccc attcgccaaa ccattccact tttccctgtt taatgagtaa
    24841 taaaccatcc tcaataaacc gcagcgcaga ggcaatctct tccgggttat cgaccgtacg
    24901 ggtgacatca ataaaactgc ctcgtaccgc ttttaacgtg tgttctcctg acatcattga
    24961 ctcctttact gttaatccat ctcttccgct gaggtaatac caggcagaac gttttcctgt
    25021 cggtagccac cagggaggat aatatttaat aaaatcgcag ttaacccgcc agcacaaata
    25081 gggttttcaa ctaatacata aatagaggct ggcaatattt taaaaatttc gggatcgtag
    25141 gagacgccaa gccctaaacc taaagaagtg gcgacaataa gtgtttcacg gcgctttaaa
    25201 ccgttggtga tgatgatgcg aatccctgcg atggcaatca tggaaaacat caacgtcatt
    25261 gcgcctccca gaactgccga gggaatggtc gtgaagaagc cgccaatcat cggaaataag
    25321 ccgaggataa ccagcattac cgcgatggtt cgcccgacat aacgtgaagc gacgccagtc
    25381 atctgaataa ccccattatt ttgcgcaaac gtggttaatg gtaatgaacc gacagcggag
    25441 gcgataacag aaaccagacc atctgccagc acgccgcctt tcagtcggga ctgatactct
    25501 tccccctgaa tggggcggcg ggaaaccatt gccgtggcgg tgatatcgcc gactgcttcc
    25561 agcacgctaa gcagataaat cgtgccaacc accaggaact gatggaagct aaaactaaag
    25621 ccgtatttga atggatgtgg gatggtgatt aacggcaaat tgcgcatact gctgaaatcc
    25681 accatgccca ggcataacga tgcgatatag ccgacacata gcccaatagc gatccctccc
    25741 atgcgtagca acggactgcg acagcagtta aagccgatca ccacaatcaa aaccaataaa
    25801 ccaacgccga gatgttcgta attgccgaag gtaccgctgc ttttggcagc aaatcctcca
    25861 ccaaaatcaa taatgccgac tttaatcagg cttaagccga tcatcagtac cacaataccg
    25921 ctgacagtag gcgtaataac ccgacgtaaa tagggcagga taaaagaaga gccgacaacc
    25981 agaaatgcgc caacgaagga gacgccgaga agcgacgaca tgattaactc ttcgtgaaaa
    26041 ccgtcgcttt tcatgctgct gcccagcgca atcatgaccg taacaaatga aaaattgact
    26101 gactgaattg agagtaggcc agaaccgacg atgccgtagc ggtttacttg taaccaggta
    26161 ccaataccag aggcgatcat cgccattgaa acaagatagg cagttgtttc agcggaaagc
    26221 tgtaaggccg cacccacgat taacgcgggg gttaccatcg gaacgaaaat tgccaacaga
    26281 tgggtaatgg caccaacgag agcctgatga aagggagggc gatcctccag ttcaaatata
    26341 aggtcagaac ctgcatggtt tatatcagac atcccttcac tccttttaaa gtaaatttga
    26401 tcatctgaat tagtaaaata aattcccacg aatttggggc gcaggacgcc ttgagaacgg
    26461 cttatctcat tttcgcagtc actatttagt catcgttcaa acagagaaac aggccgccga
    26521 agcggccatt tattacttat attttttcca gcgcggtgag tattttctcc ggtgtgaaat
    26581 gccattcgcg taaccagatg ccgcaggcat cgtgaattgc ggtagcaatc gccggagctg
    26641 cgccatttac accgatttcc gagatcgatt tcgccccgaa cgggccgact ttatcgtcgc
    26701 tcggcaccag cacagcgcgg aaatcacgcg gaatgtcacc aattttcggt gcgccgtaac
    26761 tgcgtaaatc acgcgttaac gggtgacctt cggcgtcata aatgatctct tcgctcatac
    26821 tgtggccgat agctcgcagg gtagcaccgt agatttgtcc caatgctaat tccgggttga
    26881 ccggtgtacc gcagtccagc aaggcgtaga atttatccag gcggatttca cccgtacgcg
    26941 tgttgacggc aacttcagcg aagtttgcgc catacgggaa ggcgaaatca ggcgtgatat
    27001 aactgccagt tcccaccagt gaaccaaagc cggtgccggt ttcgccttta tgagcaatat
    27061 ccccgaagct gacttcgcct ttcttgccgc gcacgacgcc cggcgttgcc agttgaacat
    27121 ctgccactgc ctcacccaac atttgcgcgc cgtggaacag aattttctcc cgcagatttt
    27181 ccgctgccaa acgcgccgcg ttacctgaga agcaagtacc ggacgaggca tatgcgcctt
    27241 tatcaaacaa cgcatgatcg gtatcaccgg agataacatg cacgtcctgc ggtgggcaat
    27301 gcagcacttc tgctgccagt ttcgtcacca cagtatccag accagtacca atatccgcac
    27361 cgccagaatg aacgataaag gtgccgtccg attccagttt gatcatgcag ttagcctgat
    27421 cgatatccgg gatacccgat ttctgcatga taatggcgac gccgcgaccg atgtgccagt
    27481 caccattttg cggtttcggt gacgaccatt ggatcatctc gcgaccctga cgcaggatct
    27541 cttctagtgc acagctggcg gcggaaggaa cggaggtcgg cgctttacct tcaccgattg
    27601 caccgagaat tttcagctct tgcccttcgt gtacccggtt acgttcgata atttccagtt
    27661 ggtcgatctg taactgttca gccagttccg ctaatgccat ggtgatagcg aagttacctt
    27721 tcggtgcgcc ataaccctga taagcaccat ttgggcaaat gttgctgtag taggtggtga
    27781 cctgaaaatc gacgttatcg cacggatata acggcagcga cagcgccggt ccgttacatg
    27841 gtacggtgag tgagtggttg ccgtaagggc cagtgttggc gcggaaatcc atcttcactg
    27901 ctgtcaggcg accatctctt ttcgctccca gtttgacggt gactttcgcg acgtgacgag
    27961 aggtgttagc aataaactct tcttcacggg tgtagcggaa cagtaccgga cgcccggtca
    28021 cgcaggttgc ccaggcgcac acttcttcca gcaggatgtc ctgtttggaa ccaaaaccgc
    28081 cgccgactcg ctctttaatg acatgaactt tatgctgttt catgcccacg aggcgcgcga
    28141 cctggcggcg taagtgccat ggtacctggg tggaggcgtg gataaccaga cgatcgccgt
    28201 ccatacgagt aaagcagata tgtgtttcag tcgggcactg ctgcgcttgc gttgagttat
    28261 aggttcgctc aatgatcaca tcggcatcgg caaagccttt gtccatatcg ccaatatgac
    28321 catgaatact ggcggcgata tttttgcggg ggcgagaacc gatcgggaag ttaatgatca
    28381 tatgctcgcc gcgctgggct gcatggctgt tatcgtcttc cagagtatct ggcgcaccag
    28441 caacatacac caccggttca tcgtgcacga caggcgcatc ttccgccatt gcttcgtcga
    28501 tcgacattac cggcttaagc acttcatatt caacgtcgat gagcttcagt gcttcgagcg
    28561 caatttcttc actttctgcg accaccgcag caacacgatc gccgacgtga cgcattttct
    28621 tgccgaacat acggcggtca agcggtgacg gttccggtgc gctctgaccc cccggggtat
    28681 agtagatatc cgggcaattc aggtgagtaa taacgtgaac gacgcccggt aaggcttcag
    28741 ctttgctgac atccagatga gtaatcagtg cgtgagcgtg tgggctacgt aacattttaa
    28801 tgacgcaggc gtcagccgtt acgcggtctt caacatagca gggtttcgcc tgcaccattt
    28861 tcgcggcatc agttttagga taatgcttgc caatgacttc aaggtcgtca cggaaagtcg
    28921 gagcgatatc aatggtggcc tgcggattat ttttacgtgc aaccgccagt tcaatgacct
    28981 gatagtattg ttgccagcct gcatcgcggc tgaacaaacc agaaagcgcg tcgtcgatct
    29041 cttcgcgggt aggtgcggcg atgcgatcga gaagatccgt gataatcaga gctgcagctg
    29101 gatcgttata accagactgc accacgccaa catcaaccat tgcctgttga accagactta
    29161 actcgttcca tttgcccaga gattctgcgg tacgaatatc tgccttctct aactgtgcgg
    29221 caataagcaa ggacgcgtta acgatattac cgttaaagat tattgcgtca gaaccggcaa
    29281 acccgaagcc atcatcactg ttgcgtacag agtgcattcc catgttaaac aacagctttt
    29341 gcacgttttc gcctggatta acggttagct cctgaggcgc gccatttaaa gtaaagtgga
    29401 tgatcatacg gcttcctccc cagcctgttg gcagtcggca tacaggtcgg ctaccagaac
    29461 tcccgtgata tagcgtttat aggccacgct gccccgcaaa tcttcctgcg ggaaaatggc
    29521 gttggcgaca gcctgttcaa gtgcattgcc ttccagattt tgtttttcga catcgtgcag
    29581 acgcagtgct ttactggcga cgccatccag cgcaattcgc ataccgtcgt ggtctgtcat
    29641 tgcaacggct gccgtcacga cggttaaacc tgcctgagaa cggctaattt tgcgggtcgc
    29701 acaggtgcga tacggatctt taatgataat ttcggttagc aggcgatcgc atgggctggc
    29761 caggtagtcc tcgattgaca gcgtttcgcc gttgccaaaa accagttcag catccagtgc
    29821 cagcaggaca ggaagcagca ccgactcttc ctgccgggcg gcgatttcac caccaatggt
    29881 cgactgatta cgaacatggc gtgagtaaac aaaaccgagg gcttcacgca gtgctgcagg
    29941 aataaatcgc gcatcacgca gtggctgcaa gcgagacatt gcaccaatcc gcagtgcacc
    30001 gttatcccag tcaacccagt ccagttccag atcctgtaag gaaatggcaa tctttttatc
    30061 ggtacgggtt ggtgtagcgt tgagtttgct gcccccggcg aaccagacgg cttcatcctg
    30121 gtagcggcgc ttcagttcca gcgcctgttc gacagagtcg ggcctgaaaa attgttcaat
    30181 catcttcttt ccttatttaa ccctcttccc tgcgggaaga gggcatctgt catttatgcc
    30241 agcgtatcca tccgacgcca catactggca gcggcttttc tggcttgtgc ataaatggaa
    30301 tcgcaatcaa agttaaactg acggtcttca tagaccatca cgccattgac catcacgctg
    30361 tgaacgctgc ctgagcccat accgaaagcg atatgcccgg cgatattgtc tgccagcagc
    30421 ggcgtcggcg agttgtaatc gcaaatggtt aaatcggctt tgtaaccggc ttccagaaga
    30481 ccaaatttcg cggcaaagtt gcggctcatc agttcgttac cgttagtcag ggctttggca
    30541 aaactgtcag gccacagcgg accacccgca tcgcgatgtt taaagaaggc aaatttcatc
    30601 tcttcaaaca tgtccgaacc aatgccgtcc gttcccaacg ccagattgcg gatgtcgcta
    30661 aggtgatggt tgtagccgac atggttgttc atgtttgaac gggcgttatg caccaggaac
    30721 gcatcgcgct gattgagtag ggcgatgtca tctttcgaca agtacagccc atgagcgacc
    30781 agcgttttgc tgtcgatgag atcgaattgc gccagtcgtg ccagcaggtc tttgccgtac
    30841 cagtggtgac tgtaggaaac gtcgtaaagg tcttccgcag cgtgaatatg caaaccacga
    30901 cctgtggctt tcacggcttc acgcagcatc tccagaccgg catctggcac ggtaaacggc
    30961 gcgtgagcac cgatatgtgc ttccaccaga tacggctcgg ttgctgcttt cttcgcctca
    31021 tcaatctgac gggcaaagcg gatgttttct tctacacctt cctgcaactc tttgatgccg
    31081 ttgttacggt cagtagtttc aaaacaggtc attgcgcgca ggccaacttt taaaaatgcg
    31141 tcgcgcaatg tggagagcga cccgccgata tacgctggag aggcatggtg atcgataacc
    31201 gatgtacatc cgctcttaat cgcttccagg gaacaaatca gcccgctgta atagagcgac
    31261 tcttcatcaa gggcgcgatc gagccgccac cagagatttt tcagcgttga gatgaaatcc
    31321 gggcaggggg cgatgtttgc cataattccg cgggaaagcc ccgagtaaaa atggttgtgc
    31381 gagcagacaa ttcccggcat cacaatccga ccatgcatct ctttgtagct ggcatcgggg
    31441 tagcgttgcg tcagggcatc gccgatagcg acaatcacat cattttcgat agcgatatca
    31501 acggcttcct gcactttcgc cgggtgtaac tgcactgcgg tgacattctt cagaatcaac
    31561 atgattacac ctccacgcgg cccagcagat aatgatgatg ctggtggaca tggctgatga
    31621 tgcggcacat atcgttcagc tccggtggta cgttgttgaa ctgaccttcg ctgtcgatgt
    31681 ttaacaccca gctttggtta ttcagacgta ctcgtacccg gcaatcttcc acaaggaagc
    31741 ctgggttgct gctgttatca aagtcttgcg acaggctgaa gacggtgatt ttgtctttgt
    31801 atggtttacc gttccacgga cagaactgag cgcagttgcc gcattcgtta cagtaagcgt
    31861 cgaggtgcag cgtctggaaa cggttctgga agcctgggac cgcaatggag acgttggcgc
    31921 ggttcgggca gacatccacg cacttgctgc aaacgtagtt acattcaagg cagcgagcag
    31981 cttcctgcgc gacaaacgca tcacgatcgt cactgttcac cagcgtgata gagatatcgc
    32041 ctttacgttg atagatttcc gccggattga cgttgttcca gtatttatcg ttctggtggg
    32101 aacggatatt ttcccgacta aggatggcat cggtcgcccg acgcgcggtt ccgacagcag
    32161 cgacaatgga ggatggtccg cgctgtacgt cgccgatcat aaagacgtca gtcagacgag
    32221 tttcgccatt atggtcgacg tctggccagc cgtttttgtc cagcggcacg cccatcgcat
    32281 tcagggcttc agtatcctgc tgttcaccaa tggcggtgat caggctgtct acatgcagtg
    32341 ttactgtttc gttggtttca accggacgac gacgaccttt ctcatccggt tcgccaagcg
    32401 acataacgcg caaggttaag gtgccatcag catcgaaacg ttccggatta ttcaggaaac
    32461 ggaactccac gccgtcgtgc aacgcttctt catactcttc gcgccatgcg ggcatctctt
    32521 gcagtgaacg acggtaaacg atcgttgctt tttttacgcc tggaacgcgt aacgccgcac
    32581 gagcgcagtc cattgcggtg ttacccgccc cgacaacgac cacatgtttg cccagcttga
    32641 gcgctgtacc cttgttgtat tcacgcagga aggggagtga tttccagaca ttttggttgt
    32701 cgcccgccag tttcacaccg ctatttttat cagtgccggt ggcaatcaga acatagtgga
    32761 agccctgatt ttttaactgt tcaacggtca aatcgggtga gcagccatac tcaaatttca
    32821 cgccgtgagc ggcaacaaaa tcgatatcgt gctgaattaa ctctgcagga atacggaact
    32881 gagggatgat atttttcacc acgccgcccg cattggcttc gcgttcaaac agcgtaaccg
    32941 gatggcccgc tctggcaagg aagtaacctg cagccagacc cgccggacct gcaccaatca
    33001 cggcaaccgg atggcgtgaa ccagaaccgg ctggtttgtg ccagcgttgc ttatattcat
    33061 cccaaccttt ttccagcgcg acttttttca gttcgcggat attcagtgca ctgtcgtaat
    33121 ccaggcgggt acagttgtat tggcactggt gatcgcaaat atgaccggta atggcgggca
    33181 gggcgttgcg ttggtagata agttccagcg cgtcggcata gcggtgttcg ccaagcagac
    33241 ggatgtattc cggaatatct tgcttgatcg cgcaggcagt aacacagggg gcaacgtagc
    33301 agtcggtcag cggcaggtct tctgccactt caatacgctc ttctggcttc cagtgtttct
    33361 gggtgtattc catggttagc gcgtctgctg ccagtctgtt cagtcgttcg acgtcaacat
    33421 ggtcaagtcc ccaggcgtcg gagccttcca gctcgcgcat gcaggcactt aaacgcagat
    33481 agccaccagg tttcagcagg tcggttgcca tagtaatagg gcgaatacct gtatcaaaaa
    33541 tatcgcggat agtcagctga ctggcaccac cggaataaga aatgggcagt ttgccgtcaa
    33601 aggcgcgaga gagaactgct gcaacattga tggagagcgg gaacagcgca cggcctgaca
    33661 tatacatctc ttcaccaggc agtgcgcctt tattgttgat ggtgccgaga gtgttagtca
    33721 gttttacgcc aaagccgagt gatttttctt ttgccagtgc catcaggcgt tccagcattt
    33781 ccagtgcttg cgtcagcttg aggtcgtgat caaatgactc ttcttttaag cctatgtaac
    33841 cgaaaccgca gacatcgagg atctcacgaa cacgcgcgta ccccagtaag gtcgggttaa
    33901 gtttcacaaa ggtgttgagc cctttttctt ccagcatgta gcggcaaatg gcttcgattt
    33961 catgcggagg acagccgtgc atggtggaga gggtgacgcc atgcaccata ctggtgggga
    34021 tgcgagcggg taaggcttgc aagctttcgc gtttttcctg caatccgtgg cgggctaaaa
    34081 atgcgtcatc ctggagtaat ttattcagcg tatcgcgata ttgcgcgaat ttcggatggt
    34141 cagatgcgtc catcatattg tcgatgaact gttgcattgg cggttgctta ataccttcga
    34201 ggttgtaacc gacgctcata ttaaagatga acgatttacc ggaatcagaa ggttggaaca
    34261 tcgcttcgag aaggtgcagg gcaaaccagg ctttgaggta ttcatcccag gctttaagca
    34321 gggtaaattc ggtagaccat tcggtgttaa agcactcgtc ttcggcatca atacagggct
    34381 tttccagctc caggcggtca agaatttgga cggtttttag ttcgatgaaa cgtccgccag
    34441 tcagccagga agtgacaata ttttgcgcga gctgcgtgtg cgggccagcg gcagggccga
    34501 cgggagtggc acaggtttca ccgaagacgc taacagtttt accttttaca ggtgagtaaa
    34561 actgttgctc gggaatacca aagattgagc gttgttgttg gtattcatca aatatgcgcg
    34621 tcaaaagttc ctcaaacgga atgggacgca taatatcccc cataaccctc tccttagctt
    34681 atacagatat cattgagtga ccggtgttga gaaatccggt ggtttagggg gatagagcaa
    34741 caatcgcacc actcttgcca ggtttggcgg gaaatataaa aaaatgtgag gcggctcgaa
    34801 acttaagggc tttttgtgaa tgaactcaca ggtgctaaat gagaattaaa atcactcctg
    34861 aaaattatta ttgccagccc atatatcact ttttctcttt tctcaaaatc agtgattcct
    34921 tatcattatg ataaagagtg ctggagggat taaatatttg aacgcaatcg tttaactacg
    34981 ttttttaaat tttagtatac cttttttgcg ctgtaataaa gtcgtccgga gtatcaatat
    35041 ctaaaattat ttctgcattt tctacttcga cggaataatg agctcccata aggagtgcct
    35101 gacgcatatt ggtgatattg gggcgcttga ttgcctgcat caggcatggt tttgacacta
    35161 aaatcggatg gccggggatg ccattatgga gtggcagtat tgcgccatca tttcgtaacg
    35221 accagatttt tctgaaaata tcgatggtga gggttggcat gtcaccgtgg gtgagaaaac
    35281 aatgttctgt ttgcactgcg ggtactgcgg cctttactga tgttagtaaa ccctgcgcat
    35341 aatctgggtt gtggataata gtgatattgc tctggttcgc ataacgttcg tgcagttcat
    35401 taccacgata gccggtgact aaaataattc ggctacaaaa ctgcaacgca tttttgatac
    35461 ttgtatcaag aattgtcccc tgttgccagg gtaacatcat tttccattgt cccattcttg
    35521 atgataatcc ggcagcggta attatacagt cgatggctga catagtggtt ccattattta
    35581 taggggctaa taacgcctga taattgcgtt acttcctgat ggattaagtt ggggataatt
    35641 tatcccagag aggtcataaa gactcgtgaa aagtatagtt gacccatcgg cattattcat
    35701 tgatttaggt gcgcagaaac gtcccactgt gatttctgtt gttggtgctg gggggaaaac
    35761 cagcttgctt ttttggctgg cagaactgct ccaggctagc ggcaggcgtg tgttaatcac
    35821 gacgacaaca catatgttta tgccaacgtc tcactggccc gtggttttct gtcgtgatcc
    35881 cgccatgctt cctcatgcgt ctcttacatc ccccatttca ttttgttttc acagctggaa
    35941 agcgaaccag ggaaaagtgc agggatttac gccagaagcg attgatgcac tggtgcaacg
    36001 accagagtgt gacgtaattc tcattgaagc agatggctcg cgtgcaatgc cgttaaaagc
    36061 gcctgatgag cacgaacctt gcatacccaa aagcagttgc tgcgtgattg ctgtgatggg
    36121 aggacatatt ttgggcgcga aagtgagcac agaaaatgtc catcgctggt cgcagtttgc
    36181 tgatattacc gggttaacac ctgatgcaac cttgcaactg agcgatctcg ttgcgctggt
    36241 tcgccaccct cagggggcgt ttaaaaacgt accgcaaggt tgtcggcgag tctggttcat
    36301 taaccgtttt tctaaatgtg agaatgcgat tgcgcaaagc gagctccttc aaccgctgca
    36361 acaacacgac gtagaggcaa tctggctggg cgatatacaa gaacatcctg caatcgcgcg
    36421 cagatttgtg aattagcggc ccgggtattc cgggttactg aaaggatatc cattgaggtt
    36481 ggtatgaata ttttcacaga ggctgcaaaa ctcgaagagc aaaattgtcc gtttgcgatg
    36541 gcgcaaattg ttgatagccg aggctcgact ccccgccatt ctgcacaaat gttagtgcgc
    36601 gccgatggtt ctatcgtcgg tacaattggt ggcggaatgg ttgagcggaa ggtgattgaa
    36661 gagtcacttc aggcattgca ggagcgtaag ccgcgattat tccatggacg tatggctcgt
    36721 aacggtgcgg atgctgtcgg gtcagattgt ggaggtgcaa tgtcagtatt tatcagcgtc
    36781 catggtatgc gtccacgtct ggtgttgatt ggcgcggggc atgtcaaccg ggcgatagcc
    36841 cagagtgcgg cgctattagg atttgatatt gccgttgccg atatttatcg cgaaagcctc
    36901 aatcctgaac tattcccacc atcaaccacg cttctccatg ctgagtcgtt tggtgcggca
    36961 gtggaagcac tggatattcg ccctgataat tttgtcctga ttgccacgaa taatcaggat
    37021 cgtgaagccc tcgacaaact cattgaacag cccattgcat ggttggggtt gctggcaagt
    37081 cgtcgcaagg ttcagctttt cctgcgtcaa ttgcgtgaga aaggcgtggc tgaagaacat
    37141 attgcccgtt tacatgcgcc cgttggttac aacataggtg cggaaacgcc gcaggagatc
    37201 gccatcagcg tgctggcaga aatattacag gtgaaaaata acgcgccggg tgggctgatg
    37261 atgaaacctt ctcatccttc cggacaccag ctggtggtga ttcgcggtgc gggagatatc
    37321 gccagtggtg tggcgctacg tctgtatcat gcgggtttta aagtgatcat gctggaagtg
    37381 gaaaaaccga cagtgattcg ttgtaccgtg gcgtttgccc aggccgtgtt cgatggcgaa
    37441 atgacggtcg aaggcgtcac tgctcgcctg gcaaccagct ctgcggaagc gatgaaactt
    37501 accgaacgcg gattcatccc tgtgatggta gatcccgcct gttcattgct tgatgaactg
    37561 aaaccgcttt gcgtggtgga cgctattctg gcgaaacaga atttggggac gcgggcagat
    37621 atggcaccag taacaatcgc gcttgggccg ggctttattg cagggaagga ttgtcatgcg
    37681 gtaattgaaa caaatcgcgg gcactggctc ggtcaggtga tttactctgg ttgtgcgcag
    37741 gagaataccg gtgttcctgg caatattatg gggcatacca cccgacgggt gatccgtgct
    37801 cctgctgcag gcattatgcg atccaacgtg aaattaggcg atctggtgaa agagggcgat
    37861 gtgattgcct ggattggtga gcatgaaatt aaagcaccgt tgacggggat ggtgcgtggc
    37921 ttgttgaacg acggcctggc agtggttggt ggttttaaaa ttggtgatat cgatcctcgt
    37981 ggtgaaaccg ctgatttcac cagcgtttct gataaagccc gggcgattgg cggcggcgta
    38041 cttgaggcgt taatgatgtt gatgcatcag ggcgtgaaag cgacaaaaga agtgctggaa
    38101 gtggcttaaa tgaaaatggc cggatggtat tacccacccg gccttaaaat tactgacaaa
    38161 tcaccgttcc ggttttgcct tcaatccctt ctttcgcttt gctcaatacg gtaatcagtg
    38221 cttcacgacc tgcgcgagag cgagcaaacg aggcggcagc ttccacttta ggcagcatag
    38281 aacctttcgc aaaatgacct tcttcgataa agcgttcagc atcacttaat gacaggcgat
    38341 cgagccactg ttcattctct ttaccgaagt taatggccac tttttctacc gcagtcagaa
    38401 tgatcagcat atcggcatca atcatttctg ctaaacgggc gctggcccag tctttatcga
    38461 taaccgcgct ggcaccacgc agatggttac cttcacgaat aacagggata ccaccaccgc
    38521 caacggtgat caccacctgg cctgcatcta ccagagcttt aaccgtttct ttttcaatga
    38581 tatcaaccgg ttttggcgag gcaacaacac ggcgatagcc gcgaccagca tcttctttca
    38641 gagtgtaacc ctgttttgtc agttgctctg cttcttgctc ggtaaagaac gaaccgatcg
    38701 gcttggtcgg gttgaggaat gccggatcgt tagcgtcgac ttcgacctga gtaaccagcg
    38761 ttgctacagg tttattaatg ccccgagaaa gcagttcttc tcttaacgcg ttttgtagat
    38821 cgtaaccaat ataaccctgg cttaaggcaa cacagacaga catcggcagc attggcgagt
    38881 gtgcttcagt ttttgcggca gcttcaaagg cctggttaat catgcccacc tgtggcccgt
    38941 taccgtgagt gacgatgacc tcatgtccct gggcaattaa atcaacaatt gcctgagaag
    39001 taatttttac cgctttcatt tgcccggcca gatcatcgcc cagcgcattc ccgcccaggg
    39061 cgagaacaat tttcttactc atttttcctc actcatcatt cttgattgtg tatagatccc
    39121 tggcagtggt tagagcacgg gagggacaaa cggtttgcgt cgcaggaaac gacctcgccc
    39181 ggctttgcct gtaaaggtgc cgtcacagaa aatcgtttca ccacgggata aggttctgac
    39241 aatcgcgccc tgacaggtaa aaccctccca tggcgagtag tcggcgttgt cgtggagatg
    39301 gcgatgctga atttgttggc tctgacgtgg gtcgataatc accacgtcgc catcggaacc
    39361 gggcgctaat aatccttttt gcggccacag gccaaacagc ctggcgggca ttgcgctggt
    39421 taattcaaca aagcgttccg gtgttatacg tcccgtcatc acgccactgg aaaacagtaa
    39481 ctgcatgcgg ttttccacgc cgggtaagcc atttgggcag cgactgaaat cgcctttaga
    39541 aatttgcagg cgttgagcca ttgagaacgt gcagtgatcg gtcgccacca cgtcaatcgc
    39601 accatcgctg atgccacacc acagtttgtc ctgctcgcgt atgttacgca gcggtgggct
    39661 aagaatgaac ttcatgccat cttctgtatc gtaactgcgt tcgtccaaca ggagatattg
    39721 tgggcaggtt tcaacccaga ctggctggtg attcgcacgg gcaagacgca gataatccag
    39781 acctaagccg ttagacaggt gcacgatata gagcggggcg ttaccggcaa tttgtgccag
    39841 gttaatcatg cgggcgatgg cttccgcttc gcattccaga gggcgactca aggcgtgata
    39901 gcgcggcgcg gttaaccctg cggcgataaa ctccgcccgc ttgctggcga tagccgcatc
    39961 attttccggg tgcacggtgg tcagcgcgcc ggattcatgc agacggcgta atgcctgcaa
    40021 aacctcgtca tcgttgagtt tgtattgata ggttaaatag agtttaaaac tgctcagccc
    40081 ttcctcgacc atcataggga tttcgtcgag gatggcgtga ttaatgtgct ggatcacacc
    40141 atgaaagctg tagtcgatga ccgccttatg ggcggcatag ccacgataaa cctccagttg
    40201 atggcgtaac cgacacccgt ttgggccaaa tcccatatgg tcaataatgg ttgttgtacc
    40261 gccacacgca gctgcgcggg taccggtaaa aaaatcatca caactgcgcg cgatgccgac
    40321 atcaatattg aaatgcgtat ggacatccac gccgccaggg aaaacgtaac agccagtggc
    40381 atcaatttct tcatacggga gctgcggcga aatattgttg cccaactggc gaacaatccc
    40441 gctttcaatc agcaaatcct gtttggcttg tccatctgcg ttaacgacag tgccgttttt
    40501 gatcaatacg cgcatagcaa actccaaaaa ccctccggcg aaccggaggg gaataagtga
    40561 agagtaatta ttcggttgcc agccagctta acgggattgc agcgtacatc gcagcacagg
    40621 tcaccaggtg agatttccag gttttttcgt taggtgcatg cgcttcaggt tctttacccg
    40681 ggccaaagcc gatcaccgga atgccgtgac ggcccatgat agatacgccg ttagttgaga
    40741 aggtccactt atcaacaacc ggcgctttgc caaacagccc ttcgtaggca ttcaccagtg
    40801 ctttaacggt gaagtgatct tcttccactt tccaggtcgg gaagtagcat tcggttgggt
    40861 aaaccaggcc agtccaggac ggacggtcgt agttgtacat agaaacaacc gcgttagctt
    40921 tctgtactgc aggcagggcg cggatttcgt ccagcgcgcc ttcccaggtt tcgccccagg
    40981 tcagacgacg gtcgattgaa actgcgcagc tgtctgctac agcgcaacgg cttggggatg
    41041 tgaagaagat ttcagaaacg gtgagggtgc ctttgccgag gaattcgtca taacccagac
    41101 gttgggagag ttcttgtaat tcgccaagaa tcggacccat tttgaaaatg gcgttatcac
    41161 cgcgttctgg tgcagaaccg tggcagctaa caccctgaac atcaatacga atttccatac
    41221 gaccgcgctg accacggtat acctggcagt cggttggttc ggtactgacc acaaattccg
    41281 ggcgaatgcc ggattgttca ataatgtact gccagcacag accgtcgcag tcttcttcct
    41341 gcacagtacc ggtaaccagc agggtatatt catcttccag accgaggtct ttaatgattt
    41401 taccggcata aaccatagat gccatgccgc cttcctggtc ggaagtaccg cgaccaccga
    41461 ttagctcatc agtttccatg ccttcgtacg gatcgaagtc ccagttcttg atgttgccaa
    41521 tgccgacggt atcgatatga gcgtccattg ccaccagacg cgggccgtgg ccgatataac
    41581 cgagaacgtt gcccatcggg tcgatttcaa ctttatcgaa gccgactttt tccatctctt
    41641 ctttaatacg atgtactacg cgtttttcgt cgcagctttc actgggaata gcaaccatgt
    41701 cgcgcaggaa gcgagtcatg tccgcctggt aatcttttgc tttttcaaga atcagtttga
    41761 atggaatatt cttagccatt atttttctcc agatttttta ctccccgttt tgcagggagt
    41821 agttccaata gggtgattaa ggtgctacag cgtgtttgcc ttcccagaca acttcgcggt
    41881 agtgcttcac gtcggtgtcg ccttcagtac tgataaccag cactacggca tctttgttca
    41941 gcgccagttt ttccatcagg ctttgacgtt gcgggtgata atgaaccgct gcgagaacgc
    42001 ccaaaccgac agcgccggat tcaccggaga tgatgcgcgg gtcgttgccg tacggattac
    42061 ccagcacgcg catacctaat gcggcaacgc tgtcctggca ggagatgaat tgggtggcgc
    42121 agttacgtag gatttcccag cccagcgggt taggttcgcc acaggccagg cccgccatga
    42181 tggtggccat atcaccgcca acgttgacga tgtcgccttt gacgccggag cgataaatac
    42241 agtcagcttt gtcaggttca acaataatgc tgtgcagatt ttgcgggctg tagacgtcga
    42301 ccagataacc cagcacacca ccggccattg caccgacacc ggcttgcagc agaacgtgcg
    42361 tcggggttac gcccatttca cgcatttgct cgacggcttc atctgccagg gttgcgtagc
    42421 cttgcatgat ccaggttggg attttggtgt aaccttccca tgccgtgtcc tgtaccactt
    42481 cccagccgtg ctgctgcgcg tgttgcatgg tcaggcgaac ggtatcgtca tagttcatat
    42541 ctgtgacgat gcactcggca ccgaggttca gaatggcgtc aacgcgttcc tgagcagaac
    42601 ctttcggcat gtaaatcacc gcattctgtc cgagttgctg tgctgcccac gccacaccgc
    42661 gcccgtggtt gccgtcggtg gtggtcgcga aagtcatttt ttcgccgatg gcatttttca
    42721 ggtgctcaaa tgacagcgtt tcgatatcaa gatgatattt ttcacacaat aattgagcga
    42781 tggcgtacgc accgccaagc attttgaagg cgttcagacc gaatcgtttt gattcgtctt
    42841 tgacgagaat tttcttcaca ccaaaaaggt ttgcgagatc gtccagcgca caaagcggtg
    42901 ttgggcgata accagctatt ttctggtgga actggcgcgc cagtttggcc tggctttgcg
    42961 aaaagagcgg tgatgtttcg ccgttgaaaa atttgttatc ggcgatatca atcttcaatg
    43021 agaaaacgga catatcctat ccttttggcc tctggaaata gagggccgac cagctgatcg
    43081 gcccgagggg ttatttcacg cgttcttgcg cttctttcag cagttgctcg agcagtgcac
    43141 ctggtttggc gtatttacgg gacaggatca tcgcggcgat gatataaggc ttccagctgg
    43201 cttctttgta ggtagcgata cggtattttt cgaatacgcc ttcagtcact tcaccttctt
    43261 tgcaggatac gccgctgata tcagctggca ggcagtgcat gtacagggct tcgccatcac
    43321 gggtcagttc catcatctct tcagtacaat gccagtcttt gtgttgcgcg ttctgtgcca
    43381 gacactgttt ttccagtgct tttaagcctt cgtggtcgtt cgcacgcagc aattcagtac
    43441 gctcttccat cactttgtaa ggtgcccatg acttcggata aacgatgtct gcgtctttga
    43501 aggcttcttc catgctggtg acctgacgga agctaccacc ggaggcttta gcattgtttt
    43561 tcgccacttc aaccacatcc gggatcaggt cgtagccttc cggatgggcc agggtgacat
    43621 ccataccgaa gcgagtcatc agaccgatga tgccttgtgg tacagagagc ggtttgccat
    43681 agcttggaga gtaggcccag gtcatggcga tttttttacc tttcaggttt tccagtgaac
    43741 caaagtgttc acgtaaccac gcgaggtcag ccattgactg agtcgggtgg tcaatatcgc
    43801 attgcaggtt cactaaagcc ggacgctgtg gcagtacacc ctgcttgtaa ccgtcatcaa
    43861 gtgcagcgcc aacttcacgc atataggcgt tgcctgcgcc cagatacata tcgtcgcgaa
    43921 taccaatagc gtcggcgcag aaggagatca tattggcggt ttcacgcacg gtttcgccgt
    43981 gagcgatttg tgatttgcct tcgtcgagat cttgttgtgc aaggccgagc aggttaagcg
    44041 cggaagcata agagaagcgg gtacgggtgg agttgtcgcg gaatacggaa atacctaatc
    44101 cactattaaa gactttggtt gagatgtttt cagcacgcag tgcttttaat gctgcggcaa
    44161 cgtccagtac ttgtttcagt tcatctggcg tctgttccca cgttaacaaa aaatctttct
    44221 cgtgaaggtg agaggtcagc gaattgatat ccttaatcag ctcattaaca gttttcatca
    44281 taaagtcccg gtagtggaat gacaggcaaa gtggtttatt tcactgtgac ttgccaaagt
    44341 gattggtgtt ttcacagcta tacctgggac taacaataag tgtgccagaa ttatccgcgc
    44401 ggctgcaaaa gactaatttg acggataatg tgtggggttg atcacaaatt gatgccagct
    44461 tctggttgtt tgtaaaatac gaaaacgttt ggcttttttt atcgcgttat ttatttgctg
    44521 ctaagtatca gaaataagat aaaggggcag gctatctctc ttcagtttga taaactcacc
    44581 g
//
LOCUS       DAFNBJ010000018        42135 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000018 DAFNBJ010000000
VERSION     DAFNBJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.018"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            329..1897
                     /gene="cydA"
                     /locus_tag="L2X49_000997"
     CDS             329..1897
                     /gene="cydA"
                     /locus_tag="L2X49_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBN4796127.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGVSAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1913..3052
                     /gene="cydB"
                     /locus_tag="L2X49_000998"
     CDS             1913..3052
                     /gene="cydB"
                     /locus_tag="L2X49_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBN4796128.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3067..3180
                     /gene="cydX"
                     /locus_tag="L2X49_000999"
     CDS             3067..3180
                     /gene="cydX"
                     /locus_tag="L2X49_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBN4796129.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3180..3473
                     /gene="ybgE"
                     /locus_tag="L2X49_001000"
     CDS             3180..3473
                     /gene="ybgE"
                     /locus_tag="L2X49_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBN4796130.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3623..4027
                     /gene="ybgC"
                     /locus_tag="L2X49_001001"
     CDS             3623..4027
                     /gene="ybgC"
                     /locus_tag="L2X49_001001"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBN4796131.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4024..4716
                     /gene="tolQ"
                     /locus_tag="L2X49_001002"
     CDS             4024..4716
                     /gene="tolQ"
                     /locus_tag="L2X49_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBN4796132.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4720..5148
                     /gene="tolR"
                     /locus_tag="L2X49_001003"
     CDS             4720..5148
                     /gene="tolR"
                     /locus_tag="L2X49_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBN4796133.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5213..6478
                     /gene="tolA"
                     /locus_tag="L2X49_001004"
     CDS             5213..6478
                     /gene="tolA"
                     /locus_tag="L2X49_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBN4796134.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6611..7903
                     /gene="tolB"
                     /locus_tag="L2X49_001005"
     CDS             6611..7903
                     /gene="tolB"
                     /locus_tag="L2X49_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBN4796135.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7938..8459
                     /gene="pal"
                     /locus_tag="L2X49_001006"
     CDS             7938..8459
                     /gene="pal"
                     /locus_tag="L2X49_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBN4796136.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8469..9260
                     /gene="cpoB"
                     /locus_tag="L2X49_001007"
     CDS             8469..9260
                     /gene="cpoB"
                     /locus_tag="L2X49_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBN4796137.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9425..9500
                     /locus_tag="L2X49_001008"
     tRNA            9425..9500
                     /locus_tag="L2X49_001008"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9458..9460,aa:Lys,seq:ttt)
     gene            9636..9711
                     /locus_tag="L2X49_001009"
     tRNA            9636..9711
                     /locus_tag="L2X49_001009"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9669..9671,aa:Val,seq:tac)
     gene            9714..9789
                     /locus_tag="L2X49_001010"
     tRNA            9714..9789
                     /locus_tag="L2X49_001010"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9747..9749,aa:Lys,seq:ttt)
     gene            9939..10014
                     /locus_tag="L2X49_001011"
     tRNA            9939..10014
                     /locus_tag="L2X49_001011"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9972..9974,aa:Val,seq:tac)
     gene            10018..10093
                     /locus_tag="L2X49_001012"
     tRNA            10018..10093
                     /locus_tag="L2X49_001012"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10051..10053,aa:Lys,seq:ttt)
     gene            10240..10315
                     /locus_tag="L2X49_001013"
     tRNA            10240..10315
                     /locus_tag="L2X49_001013"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10273..10275,aa:Lys,seq:ttt)
     gene            10349..10424
                     /locus_tag="L2X49_001014"
     tRNA            10349..10424
                     /locus_tag="L2X49_001014"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10382..10384,aa:Lys,seq:ttt)
     gene            10447..10572
                     /locus_tag="L2X49_001015"
     ncRNA           10447..10572
                     /ncRNA_class="other"
                     /locus_tag="L2X49_001015"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10821..11864
                     /gene="nadA"
                     /locus_tag="L2X49_001016"
     CDS             10821..11864
                     /gene="nadA"
                     /locus_tag="L2X49_001016"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBN4796138.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSISV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11902..12621
                     /gene="pnuC"
                     /locus_tag="L2X49_001017"
     CDS             11902..12621
                     /gene="pnuC"
                     /locus_tag="L2X49_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBN4796139.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12618..13559)
                     /gene="zitB"
                     /locus_tag="L2X49_001018"
     CDS             complement(12618..13559)
                     /gene="zitB"
                     /locus_tag="L2X49_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBN4796140.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIKMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13673..14053)
                     /locus_tag="L2X49_001019"
     CDS             complement(13673..14053)
                     /locus_tag="L2X49_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBN4796141.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14369..15421
                     /gene="aroG"
                     /locus_tag="L2X49_001020"
     CDS             14369..15421
                     /gene="aroG"
                     /locus_tag="L2X49_001020"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBN4796142.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15579..16331)
                     /gene="gpmA"
                     /locus_tag="L2X49_001021"
     CDS             complement(15579..16331)
                     /gene="gpmA"
                     /locus_tag="L2X49_001021"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBN4796143.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16536..17576)
                     /gene="galM"
                     /locus_tag="L2X49_001022"
     CDS             complement(16536..17576)
                     /gene="galM"
                     /locus_tag="L2X49_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBN4796144.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17570..18718)
                     /gene="galK"
                     /locus_tag="L2X49_001023"
     CDS             complement(17570..18718)
                     /gene="galK"
                     /locus_tag="L2X49_001023"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBN4796145.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDNFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18722..19768)
                     /gene="galT"
                     /locus_tag="L2X49_001024"
     CDS             complement(18722..19768)
                     /gene="galT"
                     /locus_tag="L2X49_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBN4796146.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19778..20794)
                     /gene="galE"
                     /locus_tag="L2X49_001025"
     CDS             complement(19778..20794)
                     /gene="galE"
                     /locus_tag="L2X49_001025"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBN4796147.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(21055..22527)
                     /gene="modF"
                     /locus_tag="L2X49_001026"
     CDS             complement(21055..22527)
                     /gene="modF"
                     /locus_tag="L2X49_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBN4796148.1"
                     /translation="MSSLQILQGAFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(22595..23383)
                     /gene="modE"
                     /locus_tag="L2X49_001027"
     CDS             complement(22595..23383)
                     /gene="modE"
                     /locus_tag="L2X49_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBN4796149.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VIMSLPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23512..23661
                     /gene="acrZ"
                     /locus_tag="L2X49_001028"
     CDS             23512..23661
                     /gene="acrZ"
                     /locus_tag="L2X49_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBN4796150.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23828..24601
                     /gene="modA"
                     /locus_tag="L2X49_001029"
     CDS             23828..24601
                     /gene="modA"
                     /locus_tag="L2X49_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN4796151.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVAIFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24601..25290
                     /gene="modB"
                     /locus_tag="L2X49_001030"
     CDS             24601..25290
                     /gene="modB"
                     /locus_tag="L2X49_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBN4796152.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25293..26351
                     /gene="modC"
                     /locus_tag="L2X49_001031"
     CDS             25293..26351
                     /gene="modC"
                     /locus_tag="L2X49_001031"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBN4796153.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26352..27170)
                     /gene="ybhA"
                     /locus_tag="L2X49_001032"
     CDS             complement(26352..27170)
                     /gene="ybhA"
                     /locus_tag="L2X49_001032"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HBN4796154.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27325..28320
                     /gene="pgl"
                     /locus_tag="L2X49_001033"
     CDS             27325..28320
                     /gene="pgl"
                     /locus_tag="L2X49_001033"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBN4796155.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28361..29314)
                     /locus_tag="L2X49_001034"
     CDS             complement(28361..29314)
                     /locus_tag="L2X49_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4796156.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29498..30550
                     /locus_tag="L2X49_001035"
     CDS             29498..30550
                     /locus_tag="L2X49_001035"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBN4796157.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDLRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30626..32059
                     /locus_tag="L2X49_001036"
     CDS             30626..32059
                     /locus_tag="L2X49_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBN4796158.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            32242..34503
                     /locus_tag="L2X49_001037"
     CDS             32242..34503
                     /locus_tag="L2X49_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBN4796159.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAQKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYKIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAQKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMGSLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34737..36020)
                     /locus_tag="L2X49_001038"
     CDS             complement(34737..36020)
                     /locus_tag="L2X49_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBN4796160.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(36155..37225)
                     /locus_tag="L2X49_001039"
     CDS             complement(36155..37225)
                     /locus_tag="L2X49_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN4796161.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTVLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(37203..37421)
                     /locus_tag="L2X49_001040"
     CDS             complement(37203..37421)
                     /locus_tag="L2X49_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBN4796162.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(37461..37628)
                     /locus_tag="L2X49_001041"
     CDS             complement(37461..37628)
                     /locus_tag="L2X49_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796163.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENISDCYDHWMLWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            37871..38473
                     /locus_tag="L2X49_001042"
     CDS             37871..38473
                     /locus_tag="L2X49_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796164.1"
                     /translation="MSYFLRKKWMVNLSGSGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINILGAVVTYAQTARDDTE"
     gene            complement(38684..38905)
                     /locus_tag="L2X49_001043"
     CDS             complement(38684..38905)
                     /locus_tag="L2X49_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBN4796165.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQQDLELISKQRGSK"
     gene            complement(39004..39219)
                     /locus_tag="L2X49_001044"
     CDS             complement(39004..39219)
                     /locus_tag="L2X49_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBN4796166.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(39296..39487)
                     /locus_tag="L2X49_001045"
     CDS             complement(39296..39487)
                     /locus_tag="L2X49_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HBN4796167.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAAFL
                     SQKLEMMVAKAEADERDQV"
     gene            complement(39460..39642)
                     /locus_tag="L2X49_001046"
     CDS             complement(39460..39642)
                     /locus_tag="L2X49_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HBN4796168.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            complement(39639..40319)
                     /locus_tag="L2X49_001047"
     CDS             complement(39639..40319)
                     /locus_tag="L2X49_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBN4796169.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSSVNITE
                     SPIIYRDENMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(40316..41101)
                     /gene="bet"
                     /locus_tag="L2X49_001048"
     CDS             complement(40316..41101)
                     /gene="bet"
                     /locus_tag="L2X49_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HBN4796170.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            complement(41107..41403)
                     /locus_tag="L2X49_001049"
     CDS             complement(41107..41403)
                     /locus_tag="L2X49_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBN4796171.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            complement(41479..41685)
                     /locus_tag="L2X49_001050"
     CDS             complement(41479..41685)
                     /locus_tag="L2X49_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HBN4796172.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
BASE COUNT        10078 a        10256 c        10930 g        10871 t
ORIGIN      
        1 agtcttggaa atgggcatca aaaagagata aattgttctc gatcaaattg gctgaaaggc
       61 ggtaatttag ctataaattg atcaccgtcg aaaaatgcaa atttgcttca acaaaaacct
      121 gtttattgta aggattttgc ggcgtaatat atacgtggga tcaatttgag tttttattaa
      181 catgtttgca acctttcttt acgccgtttt tgtgtgcatt cacatggtat gatgaaagtg
      241 ttcaaacaaa tttctattgg ggcatgcgtg tgaccctttc taacggggtt cactctcgga
      301 gtcttcatgc gatgagcaag gagtcatgat gttagatata gtcgaactgt cgcgcttaca
      361 gtttgccttg accgcgatgt accacttcct ttttgtgcca ctgacgctcg gtatggcgtt
      421 cctgctggcc attatggaaa cggtctacgt cctctccggc aaacagattt ataaagatat
      481 gaccaagttc tggggcaagt tgtttggtat caacttcgct ctgggggtgg ctaccggtct
      541 gaccatggag ttccagttcg ggactaactg gtcttactat tcccactatg taggggatat
      601 cttcggtgcg ccgctggcaa tcgaaggtct gatggccttc ttcctcgaat ccacctttgt
      661 aggtctgttc ttcttcggtt gggatcgtct gggtaaagtt cagcatatgt gtgtcacctg
      721 gctggtggcg ctcggttcaa acctgtccgc actgtggatt ctggttgcga acggctggat
      781 gcaaaaccca atcgcgtccg atttcaactt tgaaactatg cgtatggaga tggtgagctt
      841 ctccgagctg gtgcttaacc cggttgctca ggtgaaattc gttcacactg tagcgtctgg
      901 ttatgttact ggcgcgatgt tcatcctcgg tgtcagcgca tggtatatgc tgaaaggtcg
      961 tgacttcgcc ttcgctaaac gctcctttgc tatcgctgcc agcttcggta tggctgctgt
     1021 tctgtctgtt attgttttgg gtgatgaatc cggctacgaa atgggcgacg tgcagaaaac
     1081 caaactggct gctattgaag ccgagtggga aacgcaacct gcgcctgctg cctttactct
     1141 gttcggcatt cctgatcagg aagaggagac gaacaaattt gcgattcaga tcccttacgc
     1201 actgggcatc attgcaacgc gttccgtgga taccccggtt atcggcctga aagagctgat
     1261 ggtgcagcat gaagaacgca ttcgtaacgg gatgaaggcg tactctctgc tcgaacaact
     1321 gcgttctggt tctaccgacc aggcggttcg tgaccagttc aatagcatga agaaagacct
     1381 cggttacggt ctgctgctga aacgctatac gccaaacgtg gctgatgcga ctgaagcgca
     1441 gattcaacag gcaaccaaag actccatccc gcgtgtagcg ccgctgtact ttgcgttccg
     1501 tatcatggtg gcgtgtggct tcctgcttct ggcaatcatc gcgctctctt tctggagtgt
     1561 catccgcaac cgcattggcg agaaaaaatg gcttctgcgc gccgcgctgt acggtattcc
     1621 gctgccgtgg attgctgtag aagcgggctg gttcgtggct gaatatggcc gccaaccgtg
     1681 ggctatcggt gaagtgctgc cgacagctgt ggcgaactcg tcactgaccg caggcgatct
     1741 catcttctca atggtgctga tttgcggcct gtataccctg ttcctggtgg cagaattgtt
     1801 cttaatgttc aaatttgcac gcctcggccc aagcagcctg aaaaccggtc gctatcactt
     1861 tgagcagtct tccacgacta ctcagccggc acgctaagac aggagtcgtc aaatgatcga
     1921 ttatgaagta ttgcgtttta tctggtggct gctggttggc gttctgctga ttggttttgc
     1981 agtcactgac ggtttcgaca tgggggtggg catgctcacc cgtttcctcg gtcgtaacga
     2041 caccgagcgt cgaattatga ttaactccat tgcaccacac tgggacggta accaggtttg
     2101 gctgatcacc gcgggcggcg cactctttgc tgcctggccg atggtctatg ccgctgcgtt
     2161 ctccggcttc tatgtggcga tgatcctcgt gctggcgtct ttgttcttcc gtccggtcgg
     2221 ttttgactac cgctccaaga ttgaagaaac ccgctggcgt aacatgtggg actggggcat
     2281 cttcattggt agcttcgttc cgccgctggt aattggtgta gcgttcggta acctgttgca
     2341 gggcgtaccg ttcaacgttg atgaatatct gcgtctgtac tacaccggta acttcttcca
     2401 gttgcttaac ccgttcggcc tgctggcagg cgtggtgagc gtagggatga tcattactca
     2461 gggcgcaacc tatctgcaaa tgcgtaccgt gggcgaactg cacctgcgta cccgtgcaac
     2521 ggctcaggtg gctgcgctgg tgacactggt ctgtttcgca ctggctggcg tatgggtgat
     2581 gtacggtatc gatggttatg tcgtgaaatc gacaatggac cattacgcag cctctaaccc
     2641 actgaataaa gaagtggttc gtgaagctgg cgcatggctg gttaacttca acaacacgcc
     2701 aattctgtgg gctattccgg cactgggtgt ggttctgccg ctgctgacca tcctgactgc
     2761 acgtatggat aaagccgcgt gggcgtttgt gttctcctcc ctgacgctgg cctgcatcat
     2821 cctgacagcc ggtatcgcaa tgttcccgtt tgtgatgccg tccagcacca tgatgaacgc
     2881 aagtctgaca atgtgggatg caacttccag ccagctgacg cttaacgtca tgacctgggt
     2941 tgcggtggtt ctggtaccga tcattctgct ctacaccgcc tggtgttact ggaaaatgtt
     3001 cggtcgtatc accaaagaag atattgaacg taacacccac tctctgtact aagtaaggag
     3061 ctaaaaatgt ggtatttcgc atggattctg ggaacgcttc ttgcctgttc gtttggggta
     3121 atcaccgcgc tggcgcttga acacgtcgaa tcaggcaaag ccggtcaaga agacatctga
     3181 tgagtaagat tatcgcgact ttgtatgcgg taatggacaa gcgccccctg cgggcgcttt
     3241 ccttcgtgat ggcgcttctg ttagcaggat gtatgttttg ggacccatct cgttttgccg
     3301 cgaagaccag tgaactggaa atctggcatg gtttattgct gatgtgggcc gtctgtgctg
     3361 gtgtgattca cggcgtgggc tttcgtccgc agaaggttct ttggcaaggg attttttgcc
     3421 cattgcttgc cgatattgtt ctcattgtcg ggctgatttt cttcttcttt taaatcagaa
     3481 ttctctttaa aaaattatgg gccgctccag gcccataaat ttttacgctc ccttaacttg
     3541 ccctcattcc caaacctcaa tcgcgcgcgt atagtagcag cgtttaaaag ctctaacttt
     3601 tgttgcatta ccgggatgta aagtgaatac aacgctgttt cgatggccgg ttcgcgtcta
     3661 ctatgaagat accgatgccg gtggtgtggt gtaccacgcc agttacgtcg ctttttatga
     3721 aagagcacgc acagagatgc tgcgtcatca tcacttcagt cagcaggcgc tgatggctga
     3781 acgcgttgcc tttgtggtac gtaaaatgac ggtggaatat tacgcacctg cgcggctcga
     3841 cgatatgctc gaaatacaga ctgaaataac atcaatgcgt ggcacctctt tggttttcac
     3901 gcaacgtatt gtcaacgccg agaatacttt gctgaatgaa gcagaggttc tggttgtttg
     3961 cgttgaccca ctcaaaatga agcctcgtgc gcttcccaag tctattgtcg cggagtttaa
     4021 gcagtgactg acatgaatat ccttgatttg ttcctgaagg ctagccttct ggttaaactt
     4081 atcatgttga ttttgattgg tttttcaatc gcatcttggg ccattattat ccagcggacc
     4141 cgtattctta acgcagcggc gcgcgaagcc gaagcgtttg aagataaatt ctggtctgga
     4201 atcgaactct ctcgcctcta tcaagagagc caggggaaac gggataatct gactggttcg
     4261 gaacaaatct tttacagcgg gttcaaagag tttgtgcgcc tgcatcgtgc caatagccat
     4321 gcgccggaag ccgtagtgga aggggcgtcg cgtgctatgc gtatctccat gaaccgtgaa
     4381 cttgaaaatc tggaaacgca cattccgttc ctcggtacgg ttggctccat cagcccgtat
     4441 attggtctgt ttggtacggt ctgggggatc atgcacgcct ttatcgccct cggggcggta
     4501 aaacaagcaa cactgcaaat ggttgcgccc ggtatcgcag aagcgttgat tgcgactgca
     4561 attggtctgt tcgccgcaat tccggcggta atggcttata accgcctcaa ccagcgcgta
     4621 aacaaactgg aactgaatta cgacaacttt atggaagagt ttaccgcgat tctgcaccgc
     4681 caggcgttta ccgttagcga gagcaacaag gggtaagcca tggccagagc gcgtggacga
     4741 ggtcgtcgcg atctcaagtc cgaaatcaac attgtaccgt tgctggacgt actgctggtg
     4801 ctgttgctga tctttatggc gacagcgccc atcatcaccc agagcgtgga ggtcgatctg
     4861 ccagacgcta ctgaatcaca ggcggtgagc agtaacgata atccgccagt gattgttgaa
     4921 gtgtctggta ttggtcagta caccgtggtg gttgagaaag atcgcctgga gcgtttacca
     4981 ccagagcagg tggtggcgga agtgtccagc cgtttcaagg ccaacccgaa aacggtcttt
     5041 ctgatcggtg gcgcaaaaga tgtgccttac gatgaaataa ttaaagcact gaacttgtta
     5101 catagtgcgg gtgtgaaatc ggttggttta atgacgcagc ctatctaaac atctgcgttt
     5161 cccttgcttg aaagagagcg ggtaacaggc gaacagtttt tggaaaccga gagtgtcaaa
     5221 ggcaaccgaa caaaacgaca agctcaagcg ggcgataatt atttcagcag tgctgcatgt
     5281 catcttattt gcggcgctga tctggagttc gttcgatgag aatatagaag cttcagccgg
     5341 aggcggcggt ggttcgtcta tcgacgctgt catggttgat tcaggtgcgg tagttgagca
     5401 gtacaaacgc atgcaaagcc aggaatcaag cgcgaagcgt tctgatgaac agcgcaagat
     5461 gaaggaacag caggctgctg aagaactgcg tgagaaacaa gcggctgaac aggaacgcct
     5521 gaagcaactt gagaaagagc ggttagcggc tcaggagcag aaaaagcagg ctgaagaagc
     5581 cgcaaaacag gccgagttaa agcagaagca agctgaagag gcggcagcga aagcggcggc
     5641 agatgctaaa gcgaaggccg aagcagatgc taaagctgcg gaagaagcag cgaagaaagc
     5701 ggctgcagac gcaaagaaaa aagcagaagc agaagccgcc aaagccgcag ccgaagcgca
     5761 gaaaaaagcc gaggcagccg ctgcggcact gaagaagaaa gcggaagcgg cagaagcagc
     5821 tgcagctgaa gcaagaaaga aagcggcaac tgaagctgct gaaaaagcca aagcagaagc
     5881 tgagaagaaa gcggctgctg aaaaggctgc agctgataag aaagcggcag cagagaaagc
     5941 tgcagccgac aaaaaagcag cagaaaaagc ggctgctgaa aaggcagcag ctgataagaa
     6001 agcagcggca gaaaaagccg ccgcagacaa aaaagcggca gcggcaaaag ctgcagctga
     6061 aaaagccgct gcagcaaaag cggccgcaga ggcagatgat attttcggtg agctaagctc
     6121 tggtaagaat gcaccgaaaa cggggggagg ggcgaaaggg aacaatgctt cgcctgccgg
     6181 gagtggtaat actaaaaaca atggcgcatc aggggccgat atcaataact atgccgggca
     6241 gattaaatct gctatcgaaa gtaagttcta tgacgcatcg tcctatgcag gcaaaacctg
     6301 tacgctgcgc ataaaactgg cacccgatgg tatgttactg gatatcaaac ctgaaggtgg
     6361 cgatcccgca ctttgtcagg ctgcgttggc agcagctaaa cttgcgaaga tcccgaaacc
     6421 accaagccag gcagtatatg aagtgttcaa aaacgcgcca ttggacttca aaccgtaatc
     6481 gcgatgttga ctgttcggac ggtcaacatc aggcaccggt tgccacgggg ttctggtagt
     6541 tttgtgtatt ttagtttgtt aacattctgc taaattatcg tgggccatcg gtccagataa
     6601 gggagatatg atgaagcagg cattacgagt agcatttggt tttctcatac tgtgggcatc
     6661 agttctgcat gctgaagtcc gcattgtgat cgacagcggt gtagattccg gtcgtcctat
     6721 tggtgttgtt cctttccagt gggcggggcc tggtgcggca cctgaagata ttggcggcat
     6781 cgttgctgct gacttgcgta acagcggtaa atttaatccg ttagatcgcg ctcgtctgcc
     6841 acagcagccg ggtagtgcgc aggaagtaca accagctgca tggtccgcac tgggcattga
     6901 cgctgtagtt gtcggtcagg tcactccgaa tccggatggt tcttacaatg ttgcttatca
     6961 acttgttgac actggcggcg caccgggtac tgtacttgct cagaactcgt acaaagtgaa
     7021 caagcagtgg ctgcgttatg ctggtcatac cgccagtgat gaagtgtttg aaaaactgac
     7081 cggcattaaa ggtgcgttcc gtactcgtat tgcctacgtt gttcagacca acggcggtca
     7141 gttcccgtat gaactgcgcg tatctgacta tgacggttac aaccagtttg tcgttcaccg
     7201 ttcaccgcag ccgctgatgt ctccggcgtg gtcaccagac ggttctaaac tggcttatgt
     7261 gaccttcgaa agcggtcgtt ccgcgctggt tattcagacg ctggcaaatg gcgctgtacg
     7321 tcaggtggct tcattcccgc gtcacaacgg tgcacctgca ttctcgccag acggcagcaa
     7381 actggcattc gccttgtcga aaaccggtag tctgaacctg tacgtaatgg atttggcttc
     7441 tggtcagatc cgccaggtga ctgatggtcg cagtaacaat accgaaccga cctggttccc
     7501 ggatagccag aacctggcat ttacttctga ccaggccggt cgtccgcagg tttataaagt
     7561 gaatatcaac ggcggtgcgc cacaacgtat tacctgggaa ggttcgcaga accaggatgc
     7621 ggatgtcagc agcgacggta aatttatggt aatggtcagc tccaatggtg ggcagcagca
     7681 cattgccaaa caagatctgg caacgggagg cgtacaagtt ctgtcgtcca cgttcctgga
     7741 tgaaacgcca agtctggcac ctaacggcac tatggtaatc tacagctctt ctcaggggat
     7801 gggatccgtg ctgaatttgg tttctacaga tgggcgtttc aaagcgcgtc ttccggcaac
     7861 tgatggacag gtcaaattcc ctgcctggtc gccgtatctg tgataataat taattgaata
     7921 gtaaaggaat cattgaaatg caactgaaca aagtgctgaa agggctgatg attgctctgc
     7981 ctgttatggc aattgcggca tgttcttcca acaagaacgc cagcaatgac ggcagcgaag
     8041 gcatgctggg tgccggcact ggtatggatg cgaacggcgg caacggcaac atgtcttccg
     8101 aagagcaggc tcgtctgcaa atgcaacagc tgcagcagaa caacatcgtt tacttcgatc
     8161 tggacaagta cgatatccgt tctgacttcg ctcaaatgct ggatgcacat gcaaacttcc
     8221 tgcgtagcaa cccgtcttac aaagtcaccg tagaaggtca cgcggacgaa cgtggtactc
     8281 cggaatacaa catctcccta ggtgaacgtc gtgcgaacgc cgttaagatg tacctgcagg
     8341 gtaaaggcgt ttctgcagac cagatctcca tcgtttctta cggtaaagaa aaacctgcag
     8401 tactgggtca tgacgaagcg gcatactcca aaaaccgtcg tgcggtactg gtttactaag
     8461 agaattgcat gagcagtaac ttcagacatc aactattgag tctgtcgtta ctggttggta
     8521 tagcggcccc ctgggccgct tttgctcagg caccaatcag tagtgtcggc tcaggctcgg
     8581 tcgaagaccg cgtcactcaa cttgagcgta tttctaatgc tcacagccag cttttaaccc
     8641 aactccagca acaactttct gataatcaat ccgatattga ttccctgcgt ggtcagattc
     8701 aggaaaatca gtatcaactg aatcaggtcg tggagcggca aaagcagatc ctgttgcaga
     8761 tcgacagcct cagcagcggt ggtgcagcgg cgcaatcaac cagcagcgat caaagcggtg
     8821 cggcggcatc aacgacgccg acagctgatg ctggtactgc gaatgctggc gcgccggtga
     8881 aaagcggcga tgcaaacacg gattacaatg cagctattgc gctggtgcag gataaatccc
     8941 gccaggatga cgcaatggtg gcatttcaga atttcatcaa aaattaccct gattcaactt
     9001 acctgccaaa cgccaattat tggctgggtc agttaaacta caacaagggt aaaaaagatg
     9061 atgcggcgta ctattttgct tcggtagtga aaaactatcc gaagtcacca aaggctgctg
     9121 atgcgatgtt taaagtcggc gtcatcatgc aggacaaagg tgacaccgca aaagcgaaag
     9181 ccgtatacca gcaggttatc agtaaatacc ctggtactga tggcgctaaa caggcacaaa
     9241 aacgtctgaa cgcgatgtaa tgcataacac acgaccagaa gtcgcattat ttctggtcgt
     9301 gtcgtgcgaa tcataagcag ttgagtgatc tgcatcgaaa tttttgttgc gctcaagtct
     9361 gaaatcagta atatatgccg ccgttgccac gggatatcaa acaaaccgaa agcaacgaaa
     9421 aagtgggtcg ttagctcagt tggtagagca gttgactttt aatcaattgg tcgcaggttc
     9481 gaatcctgca cgacccacca atcgctaagg tggaagcggt agtaaaacgt gaaggataac
     9541 gttgcatcag caacggcccg aagggcgaga cgaagtcgag tcatcctgca cgacccacca
     9601 ctaacatagt tagttgtagt atccagcgta gtattgggtg attagctcag ctgggagagc
     9661 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc accgggtcgt
     9721 tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg aatcctgcac
     9781 gacccaccag ttttagcatc gaagacagat gttaagcgtg taggataacg ttgcgtcagc
     9841 aacggcccgt agggcgagcg aagcgagtca tcctgcacga cccaccacta attacggtgg
     9901 gttcggtgga agtagtttgt agtatccagc gcagtatcgg gtgattagct cagctgggag
     9961 agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accactcggg
    10021 tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct
    10081 gcacgaccca ccaattttaa catcaaactc agatgttaag cgtgaaggat aacgttgcgc
    10141 cagcaacggc ccgtagggca agcgaagcga gtcatcctgc acgacacacc aatcttaaag
    10201 attggccccg agtagaaatc tttcaggtaa cacccgtatg ggtcgttagc tcagttggta
    10261 gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc caccatcctg
    10321 aatgattaaa gcagcataat cccgcaaggg gtcgttagct cagttggtag agcagttgac
    10381 ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc
    10441 taaaggcgct tttttgctat ctgcgatact caaacctgca gcaggtttga gttgagcgca
    10501 gcgaaacaac ggagccgctc gcggcgacgg cccgaagggc gagcgaagcg agtcatcctg
    10561 cacgacccac caatgtaaaa aagcgcccta aaggcgcttt tttgctattc aggcatcctc
    10621 aatttcactt tgtaaacctg atgacatcgt cagagcttac tgtgcaagca actctacgtc
    10681 ggtggaatta ggcgtaattc gagtgacttt tccccaccat tcgactatcc tgtttagcat
    10741 ataaaacaaa ttacaccgat aacagcgaat attacgctaa tgtcggtttt aacgttaagc
    10801 ctgtaaaacg agatggtaag atgagcgtaa tgtttgatcc agacacggcg atttatcctt
    10861 tccccccgaa gccgacgccg ttaagcattg atgaaaaagc gtattaccgc gagaagataa
    10921 aacgtctgct aaaagaacgt aatgcggtga tggttgccca ctactatacc gatcccgaaa
    10981 ttcaacaact ggcagaagaa accggtggct gtatttctga ttctctggaa atggcgcgct
    11041 tcggtgcaaa gcatcccgct tctactttgt tagtcgctgg ggtgagattt atgggagaaa
    11101 ccgccaaaat tctcagtccg gaaaaaacaa ttctgatgcc gacacttcag gctgaatgtt
    11161 cactggatct cggctgccct gttgaagaat ttaacgcatt ttgcgatgcc catcccgatc
    11221 gtactgtcgt cgtctacgcc aacacttctg ctgcggtaaa agcgcgcgca gattgggtgg
    11281 taacttcaag catttccgtc gaacttattg atcatcttga tagtttgggt gaaaaaatca
    11341 tctgggcacc cgacaaacat ctggggcgtt acgtgcaaaa acagacgggt ggagacattc
    11401 tatgctggca gggtgcctgt attgtgcatg atgaatttaa gactcaggcg ttaacccgct
    11461 tgcaagaaga atacccggat gctgccatac tggtgcatcc agaatcacca caagctattg
    11521 tcgatatggc ggatgcggtc ggttccacca gtcaactgat cgctgctgcg aaagcattgc
    11581 cacatcagcg gcttatcgtg gcaaccgatc ggggcatttt ctacaaaatg cagcaggcag
    11641 tgccagataa agagttactg gaagcaccaa ccgcaggtga gggtgcaacc tgccgcagct
    11701 gcgcgcattg tccgtggatg gccatgaatg gccttcagtc catcgcagag gcattagaac
    11761 aggaaggaag caatcacgag gttcatgttg atgaaaggct gcgagagagg gcgctggtgc
    11821 cgctcaatcg tatgctggat tttgcggcta cactacgtgg ataacgaata ataaggcgta
    11881 acgttacgct ttgggggaaa gatggatttt tttagtgtgc agaatatcct ggtacatata
    11941 ccaatagggg caggcggtta tgatctctca tggatcgaag cggtaggcac gatcgccggg
    12001 ttgctgtgta ttggccttgc cagtctggag aagatcagca actacttctt tggcctgatc
    12061 aacgtcacct tgtttggcat tattttcttt cagattcagc tgtatgccag cctgctatta
    12121 caggtgtttt tctttgccgc gaatatttac ggttggtatg cgtggtcgcg acaaaccagt
    12181 cagaacgagg cggagttgaa aattcgctgg ttgccattgc cgaaggcact cagctggttg
    12241 gcggtttgcg ttgtttcgat tggtctgatg acggtattta tcaatccggt gtttgcattt
    12301 ttgacccgcg tggcagtcat gatcatgcaa gcattaggat tacaggttgt gatgcctgaa
    12361 ctgcaaccgg acgctttccc gttctgggat tcatgcatga tggtgttatc tatcgtggca
    12421 atgattctga tgacgcgtaa gtatgtggaa aactggctgt tgtgggtgat tattaacgtg
    12481 attagcgtcg ttatttttgc acttcagggc gtttacgcca tgtctctgga gtacatcatc
    12541 ctgaccttta ttgcgctcaa cggcagccgg atgtggatca acagcgcacg tgaaagaggc
    12601 tcacgcgcgc tgtcccatta atggtgatga tgtgaatggc cagacacccc ttcattcagg
    12661 tggcagtctg gcccatgaca aggttgatat tccatcttaa tggtggcgtg ctcaatctga
    12721 tagtgatcca tcaggtagtg ttggatctga tccaacaagg catcgtgatc gtgtggcggg
    12781 atcacctgca catgcaacgt catcaccggc ttctcgccta ccatccatac atgtacatgg
    12841 tgcacattgc ggacttccgg gatttcccgg cacatacgac gcttcagttc agcgatatcc
    12901 agcgataccg gtgcaccttc aagtaattca ttcacgctat ctttcaacag tcgccacgcg
    12961 ctacgcagaa ccagaagcga caccagtatc gagagaatgg ggtcagcagg tgtccagcca
    13021 gtccagataa taatcaacgc ggcaataata gccccaaccg aacccagcag gtccccgagc
    13081 acatgcagtg ccgctgctcg tacgttgagg tttttctctt cgctgccgtg atgaagtaac
    13141 cagaaagaaa gtatatttgc cagcaaccca gccacggcaa ttgccatcat catgccgccc
    13201 tcgaccggac gcggcgtgcg gaagcgttct atcgcctccc agacaatcaa aatggtaatt
    13261 accacgaggg ctatggcatt cacaaacgcc gcgagcgtgg tcagtcttag ccaaccgaaa
    13321 gtgtggcgaa tggtgggagg acgacgtgaa aattgcacgg cgagtagggc gaaaagcagg
    13381 gcggcagtat cggtcaacat atgacccgca tcggccagca atgccagaga accagaaaga
    13441 aaaccaccaa cgacttctac cagcataaac ccggcagtca cgccgaaagc atacaacaag
    13501 cgacgagcat tattatcttc tggcaggtgt gaggacgtgt gtgagtgtga gtgcgccatg
    13561 acgagtcatc ccttaattat tctcacttaa ctttatgaca tcatactgct tttagaagtg
    13621 aaaaattaaa agggagagac tccgctctcc cattattggc tattttgcag ggttactgcg
    13681 tggtaccgtc ggttttggta tcgacatcat tattgatgcc atcaccggtt tgtacctttt
    13741 tattgatatc cggacagcga ccatctttgc acatggtgtt cttgtgctct tcatctttgg
    13801 tcattccgtc attgttcatt gaagaaccat ccgaatgcag cattgtgcca ccagaaccgg
    13861 tatttacccc gttattgtcg acgttatttg gcgcgacatt ttcacgggcg tcaggggcta
    13921 cctggcccgc atcagctgcg gcgtttgcct ggccgttatt agtttgcgct ccgctatcgg
    13981 cagccagtgc ggcaccgctg gcaaggctta gagtggcagt cagaaataat gtggccagtt
    14041 ttgtcatttt cataggatgc tcctgttatg gtcgttatgt cggataacct cttccaacag
    14101 tgcatttgca ggtgaatata aggcattggt ttaagatttc agccaggtta tgaaacgcag
    14161 cagagaatct tgaaataatt aacaaacaaa ggagttacag ttagaaattg taggagagat
    14221 ctcgtttttc gcgacaatct ggcgtttttc ttgctaattc caggattaat ccgttcatag
    14281 tgtaaaaccc cgtttacaca ttctgacgga agatatagat tggaagtatt gcattcacta
    14341 agataagtat ggcaacactg gaacagacat gaattatcag aacgacgatt tacgtatcaa
    14401 agaaatcaaa gagttacttc ctcctgtcgc attgctggaa aaattccccg ctactgaaaa
    14461 tgccgcgaat acggttgccc atgcccgaaa agcgatccat aagatcctga aaggtaatga
    14521 tgatcgcctg ttggtggtga ttggcccatg ctcaattcat gatcctgtcg cagcaaaaga
    14581 gtatgccact cgcttgctgg cgctgcgtga agagctgaaa gatgagctgg aaatcgtaat
    14641 gcgcgtctat tttgaaaagc cgcgtaccac ggtgggctgg aaagggctga ttaacgatcc
    14701 gcatatggat aatagcttcc agatcaacga cggtctgcgt atagcccgta aattgctgct
    14761 tgatattaac gacagcggtc tgccagcggc aggtgagttt ctcgatatga tcaccccaca
    14821 atatctcgct gacctgatga gctggggcgc aattggtgca cgtaccaccg aatcgcaggt
    14881 gcaccgcgaa ctggcgtctg gtctttcttg tccggtaggt tttaaaaatg gcacagacgg
    14941 tacgattaaa gtggctatcg atgccattaa tgccgccggt gcgccgcact gcttcctgtc
    15001 cgtaactaaa tgggggcatt cggcgattgt gaataccagc ggtaacggtg attgccatat
    15061 cattctgcgc ggcggtaaag agcctaacta cagcgcgaag cacgttgctg aagtgaaaga
    15121 agggctgaac aaagcaggcc tgccagcaca ggtgatgatc gatttcagcc atgctaactc
    15181 gtccaaacaa ttcaaaaagc agatggatgt ttgtgctgac gtttgccagc agattgccgg
    15241 tggcgaaaag gccattattg gcgtgatggt ggaaagccat ctggtggaag gcaatcagag
    15301 cctcgatagc ggggagccgc tggcctacgg taagagcatc accgatgcct gcattggctg
    15361 ggaagatacc gatgctctgt tacgtcaact ggcgaatgca gtaaaagcgc gtcgcgggta
    15421 aggtttaatt gtcggatgcg ccgtcagagt ggcgtatccg atgaatcacc acaggcctga
    15481 taagtcgcgc agcgtcgcat caggcaatgt gctccattgt tagcaacaaa aaagccgact
    15541 cacttgcagt cggctttctc attttaaacg aatgacgttt acttcgcttt accctggttt
    15601 gcaaccgccg ctgctttcgc tgcgatctcg tcagcattac ccagataata gcgtttcagc
    15661 ggtttgaaat tctcgtcgaa ctcatacacc agcggcacgc cagtcgggat attaagctca
    15721 agaatctctt cttcgctcat gttatcaaga tatttcacca gcgcacgtaa agagttaccg
    15781 tgtgcagcga tgatcacgcg ctcaccgctc ttcatacgcg gcagaatagt ttcattccag
    15841 taagggatca cgcggtcaat ggtcagcgcc aggctttccg tcagcggcag ttctttttcg
    15901 ctcagtttcg cgtaacgcgg atcgtgaccc ggataacgct catcatcttt agtcagttcc
    15961 ggcggagtca ctgcaaaacc acgacgccac tgtttcacct gctcgtcgcc atacttttca
    16021 gcagtttccg ctttgttcag accctgcaac gcaccgtagt gacgttcgtt cagtttccag
    16081 gatttctcaa cgggcagcca tgcctgatcc agttcgtcca gcacattcca cagggtatgg
    16141 atagcgcgtt tcagcacaga ggtgtaagca aagtcaaagc tgtaaccttc ctctttcagc
    16201 agcttacctg ctgcttttgc ttcgcttacg cctttctcag acagatccac gtcgtaccaa
    16261 ccggtgaaac ggttttcttt gttccactga ctttcgccat gacgaaccag aaccagctta
    16321 gttacagcca tatacttact cctcaaatca ttttttaatg ataataattc tcattatatt
    16381 gccgtgacga agcaacagca atgcttacgc ataaccatag cgaaaatagt gtcgcagtgt
    16441 aaggttgttg tgaatattga gttgcaaata tgtcggtgtt tgctggtgat ttgagcaata
    16501 taaggataaa gccctcatta cgagggcttc aggtgctact cagcaataaa ctgatattcc
    16561 gtcaggctgg aatactcttc gccaggacgc aggaagcagt ccggttgcgg ccattcaggg
    16621 tggtttgggc tgtccggcag aaactcgctt tccagtgcca ggccttgcca gtcggcgtaa
    16681 ggttcagttc ctcttgatgg cgtgccgccg aggaagttgc cggagtagaa ttgcagagcc
    16741 ggagcggtgg tgtagacctt cagctgcaat ttttcatctg ctgaccagac atgcgccgcc
    16801 actttcttgc catcgccttt ggcctgtaac aagaatgcgt gatcgtaacc tttcactttg
    16861 cgctgatcgt cgtcggcaag aaactcactg gcgatgattt tggcgctacg gaaatcaaaa
    16921 gacgttccgg cgacagattt caggccgtcg tgcggaatgc cgccttcatc aaccggcaga
    16981 tattcgtccg ccagaatctg caacttgtga ttgcgcacgt cagactgctc gccgtcaaga
    17041 ttgaaataga cgtgattagt catattcacc gggcaaggtt tatcaactgt ggcgcgataa
    17101 gtaatagaga tacggttatc gtcggtcaga cgatattgca ccgtcgcgcc gagattaccc
    17161 gggaagccct gatcgccatc atctgaactc agggcaaaca gcacctgacg atcgttctgg
    17221 ttcacaatct gccagcgacg tttgtcgaac ccttccgggc cgccgtgcag ctggttaacg
    17281 ccctgacttg gcgaaagcgt cacggtttca ccgtcaaagg tataacggct attggcgatg
    17341 cggttggcat aacgaccaat agaggccccc agaaacgcag cctgatcctg atagcattcc
    17401 gggctggcac agccgagcag cgcctcgcgg acgctgccat cggaaagcgg aatacgagct
    17461 gaaagtaaag tcgcacccca gtccatcagc gtgactacca tccctgcgtt gttacgcaaa
    17521 gttaacagtc ggtacggctg accatcgggt gccagtgcgg gagtttcgtt cagcactgtc
    17581 ctgctccttg tgatggttta caaacgtaaa aagtctcttt aatacctgtt tttgcttcat
    17641 attgttcagc gacagcttgc tgtacggcag gcaccagctc ttccgggatc agcgcgacga
    17701 tacagccgcc aaatccgccg ccggtcatgc gtacgccacc tttgtcgcca atcacagctt
    17761 tgacgatttc taccagagtg tcaatttgcg gcacggtgat ttcgaaatta tcgcgcatag
    17821 aggcatgaga ttccgccatc aactcgccca tacgtttcag gtcgccttgc tccagcgcgc
    17881 tggcagcttc aacggtgcgg gcgttttcag tcaggatatg acgcacgcgt ttcgccacga
    17941 ttgggtccag ctcatgtgca acagcgttga actcttcaat ggtgacatcg cgcagagctg
    18001 gctgctggaa gaaacgcgca ccggtttcgc actgttcacg acgggtgttg tattcgctgc
    18061 caaccagggt acgtttgaag ttactgttga tgatgacgac agccacacct ttcggcatgg
    18121 aaactgcttt ggtccccagt gaacggcagt caatcagcaa ggcatgatct ttcttgccga
    18181 gcgcggaaat tagctgatcc atgatcccgc agttacagcc aacaaactgg ttttctgctt
    18241 cctgaccgtt aagcgcgatt tgtgcgccgt ccagcggcag atgataaagc tgctgcaata
    18301 cggttccgac cgccacttcg agtgaggcgg aagaacttaa cccggcaccc tgcggcacat
    18361 tgccgctgat caccatgtcc acgccgccga agctgttgtt acgcagttgc agatgtttca
    18421 ccacgccacg aacgtagtta gcccattgat agttttcatg tgcgacaatg ggcgcatcga
    18481 gggaaaactc gtcgagctga ttttcataat cggctgccat cacgcgaact ttacggtcat
    18541 cgcgtggtgc acaactgatc acggtttgat aatcaatcgc gcagggcaga acgaaaccgt
    18601 cgttgtagtc ggtgtgttca ccaatcaaat tcacgcggcc aggcgcctga atggtgtgag
    18661 tggcagggta gccaaatgcg ttggcaaaca gagattgtgt tttttctttc agactcattt
    18721 cttacactcc ggattcgcga aaatggatat cgctgactgc gcgcaaacgc tctgctgcct
    18781 gttctgcggt caggtctcgc tgggtctctg ccagcatttc ataaccaacc ataaatttac
    18841 gtacggtggc ggagcgcaac agaggcggat aaaagtgcgc gtgcagctgc cagtgttgat
    18901 tctcttcgcc attaaatggt gcgccgtgcc agcccataga gtaggggaag gagcactgga
    18961 agaggttgtc ataacgactg gtcagctttt tcaacgccag cgccaaatcg ctgcgctggg
    19021 cgtcggtcaa atcggtgatc cgcaaaacgt gggctttggg cagcagtagc gtttcgaacg
    19081 gccaggcagc ccagtaaggc acaacggcta accagtgttc ggtttcgaca acggtacggc
    19141 taccgtctgc cagctcgcgc tgaacataat ccaccagcat tggtgatttc tgttcggcaa
    19201 aatattcttt ttgcaggcgg tcttcgcgct cagcttcgtt aggcaggaag ctatttgccc
    19261 aaatctgtcc gtgcggatgc gggttagagc agcccatcgc cgcacctttg ttttcaaaga
    19321 cctgcaccca cgggtacgtt tttcccagct ctgcggtttg ctcctgccag gttttgacga
    19381 tttccgtcaa tgccgcaacg ctcagttctg gcaacgtttt actgtgatcc ggtgaaaagc
    19441 agatcacccg gctggtaccg cgcgcgctct ggcaacgcat caaaggatcg ttgctttctg
    19501 gcgcatctgg cgtgtcagac atcaaggccg caaagtcatt agtgaaaacg taagtcccgg
    19561 tgtaatcggg gtttttatcg cctgtcaccc gcacattacc tgcgcagagg aagcaatctg
    19621 gatcgtgcgc aggtaacacc tgtttggctg gcgtttcctg cgccccctgc caggggcgct
    19681 tagcgcggtg cggtgaaacc agaatccatt gcccggtgag cgggttgtag cggcgatgtg
    19741 gatgatcaac gggattaaat tgcgtcatgg tcgttcctta atcgggatat ccctgtggat
    19801 ggcgtgactg ccagtgccag gtgtcctgcg ccatttcatc gagtgtgcgc gttacgcgcc
    19861 agttcagttc acggtcggct ttgctggcgt ccgcccagta ggccggaagg tcgccctcgc
    19921 gacgcggtgc aaaatgataa ttaaccggtt tgccgcaggc tttgctgaag gcattaacca
    19981 cgtccagcac gctgttgcct acgccagcgc cgaggttgta gatgtgtacg cctggcttgt
    20041 tcgccagttt ttccatcgcc acgacgtgac cgtccgccag atccattacg tggatgtaat
    20101 cgcgtacgcc agtaccatct tcggtcggat aatcgttacc aaaaatcgcc agcgagtcgc
    20161 gacggcctac agcaacctgg gcgatgtatg gcatcaggtt attcggaatg ccttgcggat
    20221 cttcgcccat atcgcccgac ggatgcgcgc caaccgggtt gaagtagcgc agcagggcaa
    20281 tgctccagtc cggctgggct ttttgcagat cggtgaggat ctgttccacc atcagcttgc
    20341 ttttgccgta agggctttgt ggtgtgccgg tcgggaagct ttcaacgtat ggaattttgg
    20401 gctgatcgcc ataaacggtg gcggaggagc taaaaataaa gtttttgacg ttagcggcgc
    20461 gcatggcgct aatcaggcgc agagtgccgt tgacattgtt gtcgtaatat tccagcggtt
    20521 tttgtaccga ttcgccaacg gctttcagcc cggcgaagtg gatcacggtg tcgatagcgt
    20581 gatcgtgcag tatctcggtc atcaacgctt cgttacgaat atcgccttca acaaacgttg
    20641 gatgtttgcc gcctaaacgc tcgataacag gcagtacgct gcgcttactg ttacagaggt
    20701 tatcaagaat gatgacatca tgaccgtttt gcagtaattg cacacaggta tgacttccaa
    20761 tgtaaccgct accaccggta accagaactc tcataattcg ctccattagg cttatggtat
    20821 gaaataacca tagcataaca aagatgcgaa aagtgtgaca tggaataaat tagtggaatc
    20881 gtttacacaa gaatttagcc gttttttatg cgcgattaag tgattataaa acagagggtt
    20941 tatgaatgat tgcgcttttt atctgaaaaa agaagcggtt tcatgcctgc atgcgtcgaa
    21001 ccgttggccg gagagggcgc ttaggccgcc tccggcaagg tcagcactac cgactcaata
    21061 tatttttgtc agcacatagc gatagagtcc accgtccggc acgaactcaa ggcgatgggt
    21121 aatacaggca ggcgcatctt cagcgtggtg cgaaacaaac aacaattgcg tttcaccttc
    21181 gctaatcagc acatcaacaa aacggcggat aagctggcga ttgagcggat caagcccctg
    21241 tagtggttca tcgagaataa gcaacgtcgg atgtttcacc agtgcgcgga cgatcagcgc
    21301 cagacgctgc tgtccccagg aaagactatg gaacggagcg tcagccgtac gtttatcaat
    21361 gccgagaata tccagccact gctgcaccag tttttgctgg cgatccgaaa cggcctgata
    21421 aatgccaatc gaatcaaaat agccagaaag aatcacatta cgcacagtag tactgacccg
    21481 gtaatccaga tgcaaactac tgctgacgta accgatatgc tttttgatat cccagatggt
    21541 ttcgccgctg ccgcgacgtc gtccgaaaag cgtcaaatcg ttgctgtaac cttgcggatg
    21601 atcgccagta accaggctta ataacgtcga ttttcctgca ccatttggcc cgacaatttg
    21661 ccagtgttcg cctggattca cctgccagct aaggttatta agaatggggc gatcgttata
    21721 agaaaccacg ccattgttca gcacaatgcg cggttcgttg gcgggtaagg cgtgacgtgc
    21781 tgaaggttca tccggctccg gcagttgcac accttcaagc tgttcactat gcgccagttg
    21841 cgcgacgagt gcttgttgga gcagttcctc tttagcgcca gtttccgcta acgtgcaatc
    21901 cgccagcacg ccagcaaact ggacaaactc cgggatctca tcgaagcgat tgagcaccag
    21961 taccagagta ataccggact gatgtaacga ggcgagtcgc tcagccagct gctgacgtga
    22021 ggcaacatcc aggccatcga acggctcatc aagaatcaac aagtcaggct ccgacatcag
    22081 cgcctgacac agcagggttt ttcgcgtctc gccagtggaa aggtatttaa agcgtcggtc
    22141 gaggagggcg gtaataccga actgctgcgc cagttgcatg caacgcggtg catcctttac
    22201 ttcatcctga atgatctcag ccgtagtgcg tccggtgtca tcttcgccag ggccgagcat
    22261 atcggtgtta ttccgctgcc attcgtcgct gacgagtttt tgcaattgct cgaaggagag
    22321 acgagtgatg tgggaaaact ggctttgccg ttcacctttc aaaagcggaa gttcccccgc
    22381 cagcgcgcgg gccagcgccg atttcccgct tccattcgaa ccgacaaacg cccaactatc
    22441 acccgcgttt aacgttagct gaggcaattg cagcgttttt gtgtcgctaa gacgaaacgc
    22501 gccttgcaaa atttgcaacg atgacatttt atatcccact ttgtgcagcg attactgaca
    22561 gggatacgtg tttcataaca aattgtcaac acgcttagca cagcgtggcg ataatcacgc
    22621 tgtcggcatt aaagtaggcc gtgacattct gtccttgctg aagagaagtc gcttcattta
    22681 ccggcactgt ggcgcacagt gtttgcccgt ccggtagcga cattattact tcgcactgct
    22741 ctgcgccgcg ctcaatatgg ctaataatac ccggtaattg gttgtcagcg ttttgcgcga
    22801 ccgcttcgtc ctgagtaatg cctacccacg gcgcttttag caatatcaac acttctttgc
    22861 cttcatccag ccccagacgc gcgccgcttt gtgcggtaat tgcgactttc aggcgtgttt
    22921 ttccgtcagc cagtaagaca tcaacatgct gttgaacgtc atcatgatcg cgggcggtga
    22981 tggtaccgaa ccactggtta cgggcgctgg tttgcagtga aaaacgtgag atcgcggcca
    23041 acaggctgtt cagcggcagg gcgtcatcgt cacttaacac atcaaaggct ttttgctgga
    23101 tttgcgccag taagtcatag agctgaatca gtcgctgacc atagcgggtc agtactgcgc
    23161 cgccgccacc tttaccgcct gttgcgcgct cgaccagaat atgctcactt aactgattca
    23221 tctcgttaat ggcatcccag gcgcttttat agctgatacc ggcatctttc gctccctggc
    23281 taatggaacc ggaaagcgca atgtgtttta gtagcgaaat gcggcgcggg tcggcgaata
    23341 atttttgttg gagcttaagg gtgagaagga tttcggcctg cataacaatg tcctggcaaa
    23401 agtcttattg tgacggaaaa cgaacgccac gcaaagctga ccgcacaaaa ggggagtgct
    23461 tttctgtgct tagcggttag aatagtctca tgactatatc tggagttgac catgttagag
    23521 ttattaaaaa gtctggtatt cgccgtaatt atggtacctg tcgtgatggc catcatcctg
    23581 ggtctgattt acggtcttgg tgaagtattc aacatctttt ctggtgttgg taaaaaagac
    23641 cagcccggac aaaatcattg attccctgaa tgcccgctta gtcgggcatt ttctttttct
    23701 caacttcctg cttttcctgc cgatattttt tcttatctac ctcacaaagg ttagcaataa
    23761 ctgctgggaa aattccgagt tagtcgttat attgtcgcct acataacgtt acattaaggg
    23821 gttaccaatg gctcgtaaat ggttgaactt gtttgccggg gcggcactct ctttcgctgt
    23881 tgctggcaat gcactggcag atgaagggaa aatcacggtg ttcgccgccg catcactgac
    23941 taacgcaatg caggacattg ctacgcagta taaaaaagag aaaggcgtgg atgtggtttc
    24001 ttctttcgct tcgtcatcta ctctcgcccg tcagattgaa gcgggtgccc ctgcggatct
    24061 gtttatttct gccgatcaga aatggatgga ttatgcggtt gataaaaaag cgatcgatac
    24121 tgctacgcgt cagacactgc tcggcaatag cctggtcgtt gtagcaccga aagccagcga
    24181 gcagaaagat ttcaccatcg acagcaaaac taactggact tcgctgctga atggcggtcg
    24241 cctcgcggtt ggcgatccgg aacatgtccc tgctggcatt tatgcaaaag aagcactgca
    24301 aaaactgggc gcatgggata cgctctcccc gaaactggct ccggcggaag atgttcgtgg
    24361 cgcgctggcg ttggtcgaac gcaatgaagc gccgttgggc attgtttacg gttctgacgc
    24421 agttgccagc aaaggggtaa aagtggttgc catcttcccg gaagattcac ataaaaaagt
    24481 ggaatatccg gttgctgttg tggaagggca taacaatgcg acagtgaaag cattttatga
    24541 ttatctgaag ggaccgcagg ctgcggaaat ctttaaacgt tacggattta caaccaagta
    24601 atgatactga ccgatccaga atggcaggca gttttattaa gcctgaaagt ttcttccctg
    24661 gctgtgctgt ttagcctgcc gtttgggatc ttttttgcct ggttactggt gcgttgcacg
    24721 tttccgggca aagctctgct cgacagcgta ctgcatctac cgctggtgtt accgcccgtg
    24781 gtcgtcggtt acttattatt agtttcgatg ggacggcgcg gatttatcgg tgaacgtctg
    24841 tatgactggt ttggtattac cttcgccttt agctggcgcg gcgcggttct cgctgccgcc
    24901 gtcatgtcgt ttccgctgat ggtgcgggca attcgtctgg cgctggaagg ggttgatgtc
    24961 aaactggaac aggccgcaag aacactgggg gccgggcgct ggcgcgtttt ctttactatc
    25021 acgttaccgc tgaccttacc gggaattatt gttggtacgg tactggcttt tgctcgttct
    25081 ctcggtgagt ttggtgcaac catcaccttt gtgtcgaaca ttcctggtga aacgcgaacc
    25141 attccttctg ccatgtatac cctgatccag acccccggcg gggaaagtgg agcggcgaga
    25201 ctgtgcatta tttctattgc gctggcgatg atctccctgt tgatttcaga atggctggcc
    25261 agaatcagcc gtgaacgggc ggggcgctaa tcatgctgga actgaatttt tcccagacgt
    25321 tgggcaacca ttgcctgact attaatgaaa cgctgcccgc caatggcatc actgctatct
    25381 ttggcgtctc cggtgccgga aaaacttcgc tgattaacgc catcagtgga ctgacgcgcc
    25441 cgcaaaaagg gcggattgtc ctcaatgggc gggtactaaa tgatgccgaa aaaggtatct
    25501 gcctgacgcc ggaaaagcgt cgcgttggct atgtttttca ggatgcgcgg ctgttcccgc
    25561 attacaaagt gcgtggcaat ctgcgctacg gcatgtcgaa aagtatggtc gatcagttcg
    25621 ataagctggt ggcgctttta ggcattgaac cgttgcttga ccgtttacca ggcagcctgt
    25681 ccggaggcga aaaacagcgc gtggcgattg gtcgggcttt gctgacagca ccggaattgc
    25741 tgttgctgga tgaaccgctg gcgtcactgg atattccgcg taaacgcgaa ctgttgcctt
    25801 atctgcaacg gctgacacgg gaaatcaaca ttccgatgtt gtatgtcagc cattcgctgg
    25861 atgagatcct ccatctggca gacagagtga tggtactgga aaacggtcag gtgaaagcct
    25921 ttggcgcgct ggaggaagtg tggggcagta gcgtgatgaa tccgtggctg ccgaaagagc
    25981 aacaaagtag cattctgaaa gtgacggtgc ttgagcatca tccgcattac gcgatgaccg
    26041 cgctggcgct gggcgatcag catttgtggg tcaataagct ggacgaaccg ctgcaagctg
    26101 cgctacgtat ccgcattcag gcttccgatg tttctctggt tttacaaccg ccgcagcaaa
    26161 ccagcattcg taacgtattg cgggcaaaag ttgttaatag ttatgacgac aacggccagg
    26221 tggaagtgga actggaagtc ggcggtaaaa cgctgtgggc gcgtatcagc ccgtgggcca
    26281 gggatgaact ggcgatcaaa cctggcctgt ggctgtacgc gcaaattaaa agtgtgtcga
    26341 taaccgcctg attaaatcag gtggctataa atgaactggg caatgctgtc ggtggtgtta
    26401 tcaccaatca caatgttggc gcgcgctttt accgcgtcat cggcgttgcc catcgccacg
    26461 cctgtaccag cggcttccag catactgata tcattaaagt tatcgccgaa tgccacgacg
    26521 ttttccatcg accaaccttg cgcctcaacc catttcgtca aacgtttacc tttgctgttg
    26581 ccgccgcgtg caatatcaac ctgatcgtgc caggaccatt cacactccag tcccagttca
    26641 tgttcgacat gcttaccaaa atgctgcaat tgcggcaggt catcgtgcgt cagggcgaac
    26701 ttccatacgg cgttaacttg ttgcgccgtt tcagccagag aagcgacttg tgtgaaagtc
    26761 ggacgctgtt ccggcggcag ggtttgcgcc cagttagacg tgcgaatgac atgcccggtc
    26821 gggtgctcat agaccattgc atcatccaca tacatcagac cgtgaatgtg gtgttcattc
    26881 agcatctcaa tgagttgcag ggctttatta acgggcattg ggtccgcttc cagcacggtt
    26941 tttgcatgat aatcatacaa ataggtgcca ttacagcaaa tagcaggtgt atccagcgcc
    27001 agcgcctgat aaaaaggatg aatagcgacg tgatggcgac ctgtgacgat gattaattga
    27061 tagcctgctt cgcgagcgcg ggccagggct tctatcgatg aaggaagcag ggtctttttc
    27121 ggggtcaata aggtgccgtc taagtcgaga gcaatcacgc gtgtggtcat ggcgtattcc
    27181 agattaaggt taagaatttt cgtctgcgcg aatggtacac cgataccact atcggacaaa
    27241 attctgcgtt ttaattcagc attcaccgcc aaaagcgact aattttagct gttacagtca
    27301 gttgctaaat gcaaaggagc attcatgaag caaacagttt atatcgccag ccctgagagc
    27361 cagcaaattc acgtctggaa cctgaatcat gaaggcgcac tgacgctgac acaggttgtc
    27421 gatgtgccgg ggcaggtgca gccgatggtg gtcagcccgg acaaacgtta tctctatgtt
    27481 ggtgttcgcc ctgagtttcg cgtcctggcg tatagtatcg ccccggacga tggcgcactg
    27541 acctttgccg cagagtctgc gctgccgggt agtccgacgc atatttccac cgatcaccag
    27601 gggcagtttg tctttgtagg ttcttacaat gcgggtaacg tgagcgtaac gcgtctggaa
    27661 gatggcctgc cagtgggcgt cgtcgatgtg gtcgaggggc tggacggttg ccattccgcc
    27721 aatatctcac cggacaaccg tacgctgtgg gttccggcat taaagcagga tcgcatttgc
    27781 ctgtttacgg tcagcgatga tggtcatctc gtggcgcagg accctgcgga agtgaccacc
    27841 gttgaagggg ccggcccgcg tcatatggta ttccatccaa acgaacaata tgcgtattgc
    27901 gtcaatgagt taaacagctc agtggatgtc tgggaactga aagatccgca cggtaatatc
    27961 gaatgtgtcc agacgctgga tatgatgccg gaaaacttct ccgacacccg ttgggcggct
    28021 gatattcata tcaccccgga tggtcgccat ttatacgcct gcgaccgtac cgccagcctg
    28081 attaccgttt tcagcgtttc ggaagatggc agcgtgttga gtaaagaagg cttccagcca
    28141 acggaaaccc agccgcgcgg cttcaatgtt gatcacagcg gcaagtatct gattgccgcc
    28201 gggcaaaaat ctcaccacat ctcggtatac gaaattgttg gcgagcaggg actactgcat
    28261 gaaaaaggcc gttatgcggt cgggcaggga ccaatgtggg tggtggttaa cgcacactaa
    28321 ccgctgattt aaccggcgca gtctctcctg cgccggtgta ttaacctatc tcctgtaacg
    28381 catgtctctg gcgttcgacg atattggtcc acaaattgtc tttatcgtca gtccacaaat
    28441 taatcagcaa ggcaaaaaag cgttctgctg ccggagaaag cacggcatct ttgcggcgaa
    28501 taatccccaa tgtccgacgt ataacgggtt ccaccagtgg aataccaata atcgaagaat
    28561 agggcgcatg gggcattgcc agaccaggaa gcgccgaaat ccccagtccc gcctctacca
    28621 gtcctaatga cgtcgaaaga tgacgcactt cgtaaaacca gtccagcttc cagggcttgt
    28681 cggctagctg ttgttctatc aacaatcggt tgccgctgga ggaacgtacg ccaatcattt
    28741 tgtaacccac taactcctgc cattctacaa gctgcttttt ggccaatgga tggtcacgcc
    28801 gacaggccag cacgaatggc tcgttgacca gtggggtaaa atcaatggat gaatttgtga
    28861 cgttgttcat gtttatgcca aagtctgatt cgttacacag caccgattcc atgcaattat
    28921 tcgtgccttg ttccagaatc cgcactttaa tattgggata cagctcatta aatttaccga
    28981 ttgccagcgg taaaaaataa aatactgcgg tcggaataca cgctaacgtc accataccac
    29041 gatgataagc attcatatca cgaatattaa aaagcgtttc atcgaatttc tttattaatt
    29101 cccgcgcctc cgggagcaac ctttttcctg cttttgttaa cgtaaccttg cgagttgtac
    29161 gctcaaatag ttgaatatgt aaatcttcct ccatcttttt tattcttcgc gttaatgcag
    29221 gttgcgtaat attgagtaat ttagcagcat tgttaaatga actggactct gccagtatga
    29281 caaatgcctt catacttgat aattcatgct tcattattac tccggaaaat ggaagcgacg
    29341 attttgggtg gctggtcgtt aaaaatttta actgcattta gccaacttaa attaatgaaa
    29401 aaatgttatt aatcgttgag ctaaagtcat tagagatgct ttgtccttaa tgtaaccata
    29461 tcgcaataag ttatgttttt aaattgaggg cattattatg aaaaaaatac cctgcgtgat
    29521 gatgcgaggt ggaacctcga ggggcgcgtt cctgttagcg gaacatttac ccgaagatca
    29581 aacgcagcgc gataaaatat tgatggcaat tatgggttcc ggtaacgatc tggaaattga
    29641 cggtattggc ggcggtaatc cactgaccag taaagtcgcc attattagcc gttccagcga
    29701 tctgcgtgct gatgttgatt atctgtttgc tcaagttatc gtccatgagc aacgtgtcga
    29761 taccacgcct aactgcggca atatgctttc tggcgttggg gcattcgcca ttgaaaatgg
    29821 tttgattgca gcgacatcac cggtcacccg tgtgcgtatc cgcaacgtca atacgggtac
    29881 gttcatcgaa gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg gtagtgccag
    29941 aattgacggc gtaccgggta ctgccgcacc ggttgcgctc actttcctga atgccgctgg
    30001 aaccaaaacc ggaaaagttt tcccgactga taatcagatt gattattttg acgatgtccc
    30061 ggtgacctgt atcgatatgg cgatgccagt cgtcattatt ccggctgaat atctgggaaa
    30121 aacaggttat gaattaccgg cggaactgga tgccgacaaa gcattattag cccgcattga
    30181 atctatccgt ctacaagcgg gtaaagcaat gggcttaggt gatgtcagta atatggttat
    30241 ccctaaacct gtgcttattt ctccagcgca gaaaggcggg gcaattaatg tgcgttattt
    30301 tatgccgcat tcttgccatc gcgcgctggc gataaccggt gctattgcta tttccagtag
    30361 ttgtgcattg gaaggcaccg tcacccgaca aatcgtccct tctgtaggat acggcaatat
    30421 caatattgaa caccccagtg gtgcgctcga cgttcattta agtaatgaag gtcaggatgc
    30481 cacgacgtta cgcgcatctg ttattcggac gaccagaaaa atattttccg gtgaagttta
    30541 tcttccctga aaaaattcgt tgtcaagata aggataatca ataaaggact tctgtatgag
    30601 tcatacagaa agaacaggat tttaaatgaa taagaaatcg ttatggaagc taattctgat
    30661 attagcgatc ccatgtatta ttggttttat gccagctccg gcaggattaa gcgaactggc
    30721 gtgggtgctt tttggtattt acctggcggc cattgtgggg ctggttatca agcctttccc
    30781 ggaacctgtc gtactgttaa ttgccgttgc tgcctccatg gtggtggtcg gtaacttatc
    30841 cgacggtgcg tttaagacca ccgccgtatt aagcggttac tcttcaggta ctacctggct
    30901 ggtgttctcg gcgtttacct taagcgccgc atttgtgacc accggtttag gtaaacgtat
    30961 tgcctatctg ctgattggta aaatcggtaa caccacgctg ggtctgggtt acgttacggt
    31021 attcctcgat ctggtactgg ctccggcaac accgtctaac accgcgcgtg cgggcggtat
    31081 tgtgttaccg atcatcaaca gcgtggcggt ggctttgggg tccgaaccgg aaaaaagtcc
    31141 gcgtcgtgtc ggacattacc tgatgatgtc catttacatg gtcaccaaaa ccaccagcta
    31201 tatgttcttt accgcaatgg cggggaacat tctggcgctg aaaatgatca acgacattct
    31261 gcacctgcaa attagctggg gtggatgggc actggccgcc ggattgccgg gcatcattat
    31321 gctgctggtc accccactgg tgatttatac catgtatccg ccagaaatta agaaggtgga
    31381 taataaaacc atcgctaaag cgggccttgc cgaactggga ccgatgaaaa tccgcgaaaa
    31441 aatgctgctc ggtgtctttg tgctggcgct actgggctgg attttcagta agtctctggg
    31501 ggttgatgaa tccactgtgg caatcgttgt tatggcgacc atgctgctgc tgggtatcgt
    31561 tacctgggaa gacgtggtta aaaataaagg cggctggaat accttaatct ggtacggcgg
    31621 tattatcgga ttaagctcct tattatcgaa agttaaattc ttcgaatggt tagctgaagt
    31681 ctttaaaaat aacctggcat ttgatggtca cggtaacgtt gctttcttcg ttattatttt
    31741 cctcagcatt atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg ttgctatgtt
    31801 accggtattt gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa ccgcgctggc
    31861 actgttgttc tccaactcct atggcggcat ggttactcac tatggcggcg cggcaggtcc
    31921 ggtcatcttt ggcgtgggtt ataacgatat taaatcctgg tggttggtcg gtgcggtact
    31981 gacgatatta accttcctgg tgcatatcac cctcggcgtg tggtggtgga atatgctgat
    32041 cggctggaac atgctgtaaa tattctcgtc atacttcaag ttgcatgtgc tgcgtctgcg
    32101 ttcgctcacc ccagtcactt acttatgtaa gctcctgggg attcactcgc ttgtcgcctt
    32161 cctgcaactc gaattattta gagtatatcc atttattatc tttctgcgca cttcacggtg
    32221 cgcagatatc tggagcattt gatgatcaag ttatctgaaa aaggcgtgtt tctcgccagt
    32281 aataacgaaa taattgccga agaacatttc accggcgaaa ttaaaaaaga agaagcccaa
    32341 aaaggcacta ttgcctggtc tattctctct tcgcataata cgtccggaaa tatggataaa
    32401 cttaaaatta agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag
    32461 accgctaaag cgtccggtat ggaacgtttc ccgctgccgt atgtgctgac caactgccat
    32521 aactcactct gcgccgtcgg cggcactatt aacggtgatg accatgtttt tggtttatcg
    32581 gcggcccagc gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat
    32641 atgcgtgaga tgatggcagg cggcggcaaa atgatcctcg ggtcagacag ccacacccgt
    32701 tacggtgcat tagggacaat ggcagtcggt gagggcggcg gtgagttggt aaaacagctg
    32761 cttaatgaca cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa
    32821 cctgcgccgt atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa
    32881 aacggttacg tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct
    32941 accgatttcc gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc
    33001 tggcaaactg atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac
    33061 tgccagctta accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc
    33121 gccatcaaac caatgattgc gctgccgttc cacccgagca acgtgtataa aatcgacaca
    33181 ctgaaccaga acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg
    33241 cacggtaaag ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag
    33301 caggggatta tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat
    33361 gcactgcgcg gtcaatcctg tggcaatgac accttctcgc tggcagttta cccgtcatca
    33421 cagccggtgt ttatggatct cgcccaaaaa ggtgtggtag cagatttgat tggcgcaggc
    33481 gcaatcatca gaaccgcgtt ctgcggccca tgctttggcg cgggcgatac gccaatcaac
    33541 aacggtttga gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca
    33601 gctaatgggc agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg
    33661 gcaaacggtg gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag
    33721 tacgccttcg atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca
    33781 actcagcaac cgctgattta cggaccgaac attaaagact ggccggaatt gggtgcgctg
    33841 actgacaata tcgtcctgaa agtgtgctcg aagatcctcg acgaagtgac caccaccgac
    33901 gaactgattc cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcggag
    33961 tttaccctgt cacgccgcga tcccggttat gttggcagaa gtaaagcgac tgctgagctg
    34021 gaaaatcagc gtctggcggg gaatgtcagc gagctgacag aggtgtttgc gcgcattaag
    34081 cagattgctg gtcaggagca tattgatccg ctgcaaactg aaattggcag catggtatat
    34141 gcggtgaaac caggcgatgg ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt
    34201 ggcggtctgg cgaatattgc cgaagagtac gcgactaaac gctaccgttc taacgtcatc
    34261 aactggggga tgttaccgct gcagatggcg gaagtgccaa cctttgaagt gggggattac
    34321 atttacatcc ctggcattaa agcggcgctg gataatccgg gtacgacgtt taaaggttat
    34381 gtgatccatg aagatgcgcc ggtaacggaa attacgctct atatgggaag tctcactgct
    34441 gaagagcgcg agattatcaa ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg
    34501 taaaaagcgc catgtgaatg taggtcgcat tcggcactta ttgtcggatg cgatgcttgc
    34561 gcatcttatc cgacctacga atcgcatcga atctgtaggc cagataaggc attttcgcag
    34621 catccggcac ttattgtcgg atgcgatgct tgcgcatctt atccgaccta caaatcgcat
    34681 cgaaccgtag gccggataag gcgtttacgc cgcatccggc aaatagttaa ttgctcttac
    34741 ttcttcgcct ctgcaaccac tttactaccc acgccgcggt tattgtattc ccacatgcgg
    34801 ttgtagttag tgtcattcag attgcgctgt atttcgtcgt tatcatctac gctgccggta
    34861 ttacccgcaa acggacgatt agagatcacc gcatcggccc acggtttagc cgtgttaaaa
    34921 ccttcgttga tggcgctatc acggatcacc acctgaccgt tggtattggc atcaacatcc
    34981 agcgagcggc ccagttgcgc cacaccatca ccgaaagcat tgaaacggct gtttacggcg
    35041 aggaaaccgt agtaaatgtt ggacagcgta gccggtgcaa acacatacgc ttcttgctga
    35101 gtacgtgagt tcaccacgcg gaattcggtg ttatcgaaca ccactgcgcc gcgaccagaa
    35161 acgatatcca catccccttc aatgtagctg ttggtcacca gcgtacgcgg ctgacgattc
    35221 gtttccagac ggttctgcac accgctgttg gtgacaaaga aggtgttctg acgaccgaga
    35281 atgttaacgt tgttaatctg tacctggtca ccatcagtac gcagtgccac cgccggatgg
    35341 ttacctgcat ctacgctatc gcccagcgtg ttttcgatgg tcagattttg cagttgcagg
    35401 ccattgtttt gtgaccagaa gaccgcagag cagagaacac cgatactgtc gctgcgtttg
    35461 ctctggcagc tatcgtacat ataccacgct ggtttacctg gcatatattt gccgcgcggg
    35521 ttgacgtcgt gacgccagtc ggcagggctc atgccaccat caagggaaag cccaatcttc
    35581 acatcaatcg gtttttcacc tgtaccgtac agagtaattc cacccggagc ggcagggaca
    35641 tataccgttc cctgatactc accaggcatc acggcaatat actggcgctt gttggtacgc
    35701 ttgataattg ccgcatctac cgccgcctga atcgtggtat gcgttacacc ttgagtgccc
    35761 gccgggccga caacaaagtc aggttgcgca ggcagggtaa tcggggaagg attccacgct
    35821 gctgcacctg gtgtcaggga tgcaaaatag tgttgagcat cgaaattctg cgcttctttt
    35881 gccgacagaa tcgggcgaga agaggtacca ggcgcggttt gatcagaagg acgttgatcg
    35941 ggcggggttg agctacaggc ggtcagcgtc acgccaaaag ccaatgccag cgccagacgg
    36001 gaaactgaaa atgtgttcac aggttgctcc gggctatgaa atagaaaaat gaatccgttg
    36061 aagcctgctt ttttatacta agttggcatt ataaaaaagc attgcttatc aatttgttgc
    36121 aacgaacagg tcactatcag tcaaaataaa atcattattt gatttcaatt ttgtcccact
    36181 ccctgcctct gtcatcacga tactgtgatg ccatggtgtc cgacttatgc ccgagaagat
    36241 gttgagcaaa cttatcgctt atctgcttct catagagtct tgcagacaaa ctgcgcaact
    36301 cgtgaaaggt aggcggatcc ccttcgaagg aaagacctga tgcttttcgt gcgcgcataa
    36361 aataccttga tactgtgccg gatgaaagcg gttcgcgacg agtagatgca attatggttt
    36421 ctccgccaag aatctctttg catttatcaa gtgtttcctt cattgatatt ccgagagcat
    36481 caacatgcaa tactgttggg atggcaattt ttacgcctgt tttgctttgc tcgacataaa
    36541 gatatccatc tacgatatca gaccacttca tttcgcataa atcaccaact cgttgcccgg
    36601 taacaacagc cagttccatt gcaagtctga gccaacatgg tgatgattct gctgcttgat
    36661 aaattttcag gtattcgtca gccgtaagtc ttgatctcct tacctctgat tttgctgcgc
    36721 gagtggcagc gacagggttt gttgttatat ggccttcagc tattgcctct cggaatgcat
    36781 cgctcagtgt tgatctgatt aacttggctg acgccgcctt gccctcgtct atgtatccat
    36841 tgagcattgc cgcaatttct tttgtggtga tgtcttcaag tggagcatca ggcagacccc
    36901 tccttattgc tttaattttg ctcatgtaat ttatgagtgt cttctgcttg attcctctgc
    36961 tggccaggat tttttcgtag cgatcaagcc atgaatgtaa cgtaacggaa ttatcactgt
    37021 tgattctcgc tgtcagaggc ttgtgtttgt gtcctgaaaa taactcaatg ttggcctgta
    37081 tagcttcagt gattgcgatt cgcctgtctc ggcctaatcc aaactcttta cccgtccttg
    37141 ggtccctgta gcagtaatat ccattgtttc ttatataaag gttagggggt aaatcccggc
    37201 gctcatgact tcgccttctt cccatttctg atcctcttca aaaggctacc tgttactggt
    37261 cgatttaagt caacctttac cgctgattcg tggaacagat attctcttcc atccttaacc
    37321 ggaggaggga atatcctgca ttcgcgcacc catcgacgaa ctgtttcaag gcttcttggg
    37381 cgtcgctggc gagcgttcca ctcctgaagt gtcaagtaca tcgcaaagtc tccgcaatta
    37441 cacgcaagaa aaaaccgcca tcaggcggct tggtgttctt tcagttcttc aattcgaata
    37501 ttggttacgt ctgcatgtgc tatctgcgcc cacagcatcc agtggtcata gcagtcgctg
    37561 atgttctcgg cttcgataac tctgttgaat ggttctccat tccattcacc tgtgactcgg
    37621 aagtgcattt atcatctcca taaaacaaaa cccgccgtag cgagttcaga taaaagaaat
    37681 cctcgtcagt gcgaggatgc tgttcattgc tgctatacac ttttttgctc tcaacgtaag
    37741 cggtagccca ttctgttggg ttggtgcagt tgcttttagg aaatgctatt taccccttaa
    37801 atgtcggctg aaagagctaa aatccatgca aaaaatttac gcaattttgt gtattattgt
    37861 gcagtaagta atgagctatt ttctgcgcaa aaaatggatg gtaaatttgt ccgggtcagg
    37921 aaaaatttta tgggcgctaa acatgaaaaa agattcgtat ccttatttga tttgcatgac
    37981 agtttcaggg ctgatcttta ttttcctttt cttctggtgg cgggcagata tctacagggt
    38041 cacgtttctt aatcagagta tatcccacta ttacattctg tttagcatgg gaatagcttt
    38101 tctgttatct ctgttttggg ttaagaaggg gatagtaaaa caaagcggct ggaagagtct
    38161 gtcagcatac cttaaggttt atgcagggat gtgcatattt gctggatttt ttctgattat
    38221 accccttacg acactaactt attttttgcc tggagagaca tcgtcttatg ttgcaccgta
    38281 tcggtatact tccggtagtt caaaaagttg ttctggagct gaggtggatg accccgatct
    38341 acatgagaat attcgcattt gctatccgta tggcaattat gagtacgata atattatcta
    38401 tgttgaaaag aaaattaata tattaggtgc ggtagtgaca tatgcacaga ccgcgcgtga
    38461 tgatactgaa tgatatagta tatagcgggc aagttttagt taatttatcg aggtaatata
    38521 atttacctcg actcgtttgt tctggtatta atatttcgct ttacgaccga tttttatctg
    38581 atgatatcat gcggttttca tatactgact tactgtcttt tctccgttag cgattttctc
    38641 ctgctcagcg atgattttat ctttggcttc tagttaattt cgctcacttc gaacctctct
    38701 gtttactgat aagctccaga tcttgctggc aactggcaca agtccgacaa ccctgaacgg
    38761 ccagtcgtct tcgttcatct atcggatcgc cacactcaca acaatgagtg gcagatatag
    38821 cctggtggtt caggcggcgc atttttattg ctgtgttgcg ctgtaattct tcaatttctg
    38881 atgctgaatc aattatgtct gccatctttc attaatccct gaattgttgg ttaatacgct
    38941 tgagggtgaa tgcgaataat aaaaaaggag cctgtagctc cctgatgatt ttgcttttca
    39001 tgttcaccgt tccttaaaga cgccgttcaa catgccgatc gccaggctta aatgagtcgg
    39061 tgtgaatccc atcagcgtta ccgtttcgcg gtgcttcttt agtacgctac ggcaaatgtc
    39121 atcgacgttt ttatccggaa actgctgtct ggcttttttg atttcagaat tagcctgacg
    39181 ggcaatgctg cgaagggcgt tttcttgctg aggtgtcatt gaacaagccc catgtcggca
    39241 agcataagca cacagaatat gaagcccgct gccagaaaaa tgcattcagt ggttgtcata
    39301 cctggtctct ctcatctgct tctgctttcg ccaccatcat ttccagcttt tgtgaaagga
    39361 atgcggctaa cgtatgaaat tcttcgtctg tttctactgg tattggcaca aacctgactc
    39421 caatttgagc gaggctatgt gccatctcga tactcgttct taactcaacg ggagatgctt
    39481 tgtgcataca gctccccgtt tattatttat ctcctcagcc agccgctgtg ctttcagggg
    39541 atttctgata acagaaaggc cgggaaatac ccagcctcgc tttgtaacgg agtagacgaa
    39601 agtgatcgca cctacccgga tattatcgtg aggatgcgtc atcgccattg ctccccaaat
    39661 acaaaaccaa tttcagccag tgcctcgtcc attttttcga tgaactccgg caccatctcg
    39721 tcaaaactcg ccatgtactt ttcatcccgc tcaaccacga cataatgcag gccttcacgc
    39781 ttcatgcgcg ggtcatagtt ggcaaagtac caggcatctt ttcgcgtcac ccacatgctg
    39841 tactgcacct gggccatgta agccgatttt attgcctcga aaccaccgag ccggaatttc
    39901 atgaaatccc gggaggtaaa cgggcatttc agttcaaggc cgttgccgtc actgcataaa
    39961 ccatcgggag agcaggcggt gcgcatattt tcgtcgcgat agatgatcgg ggattcagta
    40021 atattcacgc tggaagtgaa ttcaaacagg gttctggcgt cgttctcgta ctgttttccc
    40081 caggccagcg ccttagcatt aacttccgga gccacaccgg tgcaaacctc agccagcagg
    40141 gtgtggaagt aggacatttt catgtcaggc cacttctttc ctgagcgggg ctttgctatc
    40201 acgttgtgaa cttctgaagc ggtgatgacg ccgagccgta atttgtgcca tgcatcatcc
    40261 ccctgttcga cagctctcac gtcgatcccg gtacgctgca ggataatgtc cggtgtcatg
    40321 ctgccacctt ctgctcagtg gctttctgtt taaggaatcc aagagctttc actgcttcgg
    40381 cctgtgtcag ttctgacgat gcgcgaatgt cgcggcgaaa tatctgggaa cagagcggca
    40441 ataagtcgtc atcccatgtt ttatccaggg cgatcagcag agtgttaatc tcctgcatgg
    40501 tttcatcgtt aaccggagtg atgtcgcgtt ccggctgacg ttctgcagtg tatgcagtat
    40561 tttcgacaat gcgctcggct tcatccttgt catagatacc agcaaatccg aaggccagac
    40621 gggcacactg aatcatggct ttatgccgta acatccgttt gggatgcgac tgccacggcc
    40681 ccgtgatttc tctgccttcg cgggttttga atggttcgcg gcggcattca tccatccatt
    40741 cggtaacgca gatcggatga ttacggtcct tgcggtaaat ccggcatgta caggattcat
    40801 tgtcctgctc aaagtccatg ccatcaaact gctggttttc attgatgatg cgggaccagc
    40861 catcaacgcc caccaccgga acaatgccgt tctgcttatc agggaaggcg taaatttctt
    40921 tcgtccacgg attaaggccg tactggttgg cgacgatcag caatgcgatg aactgcgcat
    40981 cgctggcatc acctttaaat gccgtctggc gaagagtggt gatcagttcc tgtgggtcga
    41041 cagaatccat gccgacacgt tcagccagct tcccagccag cgttgcgagt gctgtactca
    41101 tccgttttat acctctgaat caatatcaac ctggtggtga gcaatggttt caaccatgta
    41161 ccggatgtgt tctgccatgc gctcctgaaa ctcaacatcg tcatcaaacg cacgggtaat
    41221 ggcttttttg ctggccccgt ggcgttgcaa atgatcgatg catagcgatt caaacaggtg
    41281 ctggggcagg cctttttcca tgtcgtctgc cagttctgcc tctttctctt cacgggcgat
    41341 ctgctggtag tgacgcgccc agctctgagc ctcaagacga tcctgaatgt aataagcgtt
    41401 catggctgac ctcctgaaaa tggctgtgaa aatatcgccc gcgaaatgcc aggctgatta
    41461 ggaaaacagg aaaggggatt agtgattcag gccgttaccg cgtccgtcga gaaaaacttc
    41521 cacgagcaaa tcacgggtat aagtgcgctc gatgccgcga tgcagataaa gccgtccgcg
    41581 taaattagct gatgcagtcc aggtaccatc tttgtgtttg accagcattc ctggcatgac
    41641 cgcgccgcga ttaacggtct gcgttccgta atgttgatga accataaaaa ctcctgcccg
    41701 taagctgggc tgctgaacat atagagactt ctgcgcgtat tcaggcggtg gatggccgcc
    41761 ggttgtcata actaagccgc ctcgttgaag cgactaaggt atgaaatgtt gagttgattt
    41821 cagctggtca caccgacgtt cacgcgtccg tttcacccct cgcactcccc gaagcctgct
    41881 gaaattcaaa ctgcggatct aagcggtcat cgcaacggtg aatcaggtgg ttgccgtatc
    41941 gttgtgttgt tgcgatgaac ctatttaaaa ctatagttgt tttaccgtca acaacaaaag
    42001 ttgttttatt ggttgtttta gatataactg gttgtattta ggatggattt attttgtgac
    42061 ttgaatcgca tagcgataac tgaagcgagg ttatggtggt tttttgagcg gtttgtgtga
    42121 tgaggggagg caaaa
//
LOCUS       DAFNBJ010000019        41573 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000019 DAFNBJ010000000
VERSION     DAFNBJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.019"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     repeat_region   4..520
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4..31
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaacc"
     gene            complement(602..1639)
                     /gene="iap"
                     /locus_tag="L2X49_001051"
     CDS             complement(602..1639)
                     /gene="iap"
                     /locus_tag="L2X49_001051"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBN4796173.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            1891..2799
                     /gene="cysD"
                     /locus_tag="L2X49_001052"
     CDS             1891..2799
                     /gene="cysD"
                     /locus_tag="L2X49_001052"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBN4796174.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            2801..4228
                     /gene="cysN"
                     /locus_tag="L2X49_001053"
     CDS             2801..4228
                     /gene="cysN"
                     /locus_tag="L2X49_001053"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBN4796175.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEKTFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMAWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4228..4833
                     /gene="cysC"
                     /locus_tag="L2X49_001054"
     CDS             4228..4833
                     /gene="cysC"
                     /locus_tag="L2X49_001054"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBN4796176.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            4883..5206
                     /locus_tag="L2X49_001055"
     CDS             4883..5206
                     /locus_tag="L2X49_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBN4796177.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            5400..5711
                     /gene="ftsB"
                     /locus_tag="L2X49_001056"
     CDS             5400..5711
                     /gene="ftsB"
                     /locus_tag="L2X49_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBN4796178.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            5730..6440
                     /gene="ispD"
                     /locus_tag="L2X49_001057"
     CDS             5730..6440
                     /gene="ispD"
                     /locus_tag="L2X49_001057"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBN4796179.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            6440..6919
                     /gene="ispF"
                     /locus_tag="L2X49_001058"
     CDS             6440..6919
                     /gene="ispF"
                     /locus_tag="L2X49_001058"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBN4796180.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYERGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            6916..7965
                     /gene="truD"
                     /locus_tag="L2X49_001059"
     CDS             6916..7965
                     /gene="truD"
                     /locus_tag="L2X49_001059"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBN4796181.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            7946..8707
                     /gene="surE"
                     /locus_tag="L2X49_001060"
     CDS             7946..8707
                     /gene="surE"
                     /locus_tag="L2X49_001060"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBN4796182.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVICSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            8701..9327
                     /gene="pcm"
                     /locus_tag="L2X49_001061"
     CDS             8701..9327
                     /gene="pcm"
                     /locus_tag="L2X49_001061"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBN4796183.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            9467..10606
                     /gene="nlpD"
                     /locus_tag="L2X49_001062"
     CDS             9467..10606
                     /gene="nlpD"
                     /locus_tag="L2X49_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBN4796184.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            10669..11661
                     /gene="rpoS"
                     /locus_tag="L2X49_001063"
     CDS             10669..11661
                     /gene="rpoS"
                     /locus_tag="L2X49_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBN4796185.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(11755..13119)
                     /locus_tag="L2X49_001064"
     CDS             complement(11755..13119)
                     /locus_tag="L2X49_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HBN4796186.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(13208..13984)
                     /locus_tag="L2X49_001065"
     CDS             complement(13208..13984)
                     /locus_tag="L2X49_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBN4796187.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(13989..14627)
                     /locus_tag="L2X49_001066"
     CDS             complement(13989..14627)
                     /locus_tag="L2X49_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBN4796188.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(14624..15886)
                     /locus_tag="L2X49_001067"
     CDS             complement(14624..15886)
                     /locus_tag="L2X49_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HBN4796189.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(15883..16791)
                     /locus_tag="L2X49_001068"
     CDS             complement(15883..16791)
                     /locus_tag="L2X49_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBN4796190.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            16987..17754
                     /locus_tag="L2X49_001069"
     CDS             16987..17754
                     /locus_tag="L2X49_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBN4796191.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(17805..18461)
                     /gene="pphB"
                     /locus_tag="L2X49_001070"
     CDS             complement(17805..18461)
                     /gene="pphB"
                     /locus_tag="L2X49_001070"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBN4796192.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(18567..21128)
                     /gene="mutS"
                     /locus_tag="L2X49_001071"
     CDS             complement(18567..21128)
                     /gene="mutS"
                     /locus_tag="L2X49_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBN4796193.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            21204..21338
                     /locus_tag="L2X49_001072"
     CDS             21204..21338
                     /locus_tag="L2X49_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796194.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            21415..21768
                     /locus_tag="L2X49_001073"
     CDS             21415..21768
                     /locus_tag="L2X49_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBN4796195.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(21805..23883)
                     /gene="flhA"
                     /locus_tag="L2X49_001074"
     CDS             complement(21805..23883)
                     /gene="flhA"
                     /locus_tag="L2X49_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBN4796196.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(23957..24967)
                     /gene="hypE"
                     /locus_tag="L2X49_001075"
     CDS             complement(23957..24967)
                     /gene="hypE"
                     /locus_tag="L2X49_001075"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBN4796197.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(24964..26085)
                     /gene="hypD"
                     /locus_tag="L2X49_001076"
     CDS             complement(24964..26085)
                     /gene="hypD"
                     /locus_tag="L2X49_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBN4796198.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTNAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(26085..26357)
                     /gene="hypC"
                     /locus_tag="L2X49_001077"
     CDS             complement(26085..26357)
                     /gene="hypC"
                     /locus_tag="L2X49_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBN4796199.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(26348..27220)
                     /gene="hypB"
                     /locus_tag="L2X49_001078"
     CDS             complement(26348..27220)
                     /gene="hypB"
                     /locus_tag="L2X49_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBN4796200.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIAEAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(27224..27574)
                     /gene="hypA"
                     /locus_tag="L2X49_001079"
     CDS             complement(27224..27574)
                     /gene="hypA"
                     /locus_tag="L2X49_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBN4796201.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27786..28247
                     /gene="hycA"
                     /locus_tag="L2X49_001080"
     CDS             27786..28247
                     /gene="hycA"
                     /locus_tag="L2X49_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBN4796202.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            28372..28983
                     /gene="hycB"
                     /locus_tag="L2X49_001081"
     CDS             28372..28983
                     /gene="hycB"
                     /locus_tag="L2X49_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBN4796203.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            28980..30806
                     /gene="hycC"
                     /locus_tag="L2X49_001082"
     CDS             28980..30806
                     /gene="hycC"
                     /locus_tag="L2X49_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBN4796204.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQALAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            30809..31732
                     /gene="hycD"
                     /locus_tag="L2X49_001083"
     CDS             30809..31732
                     /gene="hycD"
                     /locus_tag="L2X49_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBN4796205.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            31750..33459
                     /gene="hycE"
                     /locus_tag="L2X49_001084"
     CDS             31750..33459
                     /gene="hycE"
                     /locus_tag="L2X49_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBN4796206.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            33469..34011
                     /gene="hycF"
                     /locus_tag="L2X49_001085"
     CDS             33469..34011
                     /gene="hycF"
                     /locus_tag="L2X49_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBN4796207.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            34011..34778
                     /gene="hycG"
                     /locus_tag="L2X49_001086"
     CDS             34011..34778
                     /gene="hycG"
                     /locus_tag="L2X49_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBN4796208.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            34775..35185
                     /gene="hycH"
                     /locus_tag="L2X49_001087"
     CDS             34775..35185
                     /gene="hycH"
                     /locus_tag="L2X49_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HBN4796209.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            35178..35648
                     /gene="hycI"
                     /locus_tag="L2X49_001088"
     CDS             35178..35648
                     /gene="hycI"
                     /locus_tag="L2X49_001088"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBN4796210.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            35673..36434
                     /locus_tag="L2X49_001089"
     CDS             35673..36434
                     /locus_tag="L2X49_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796211.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            36608..36919
                     /locus_tag="L2X49_001090"
     CDS             36608..36919
                     /locus_tag="L2X49_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HBN4796212.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            36916..37335
                     /locus_tag="L2X49_001091"
     CDS             36916..37335
                     /locus_tag="L2X49_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN4796213.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(37449..38873)
                     /gene="ascB"
                     /locus_tag="L2X49_001092"
     CDS             complement(37449..38873)
                     /gene="ascB"
                     /locus_tag="L2X49_001092"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HBN4796214.1"
                     /translation="MSVFPESFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(38882..40339)
                     /gene="ascF"
                     /locus_tag="L2X49_001093"
     CDS             complement(38882..40339)
                     /gene="ascF"
                     /locus_tag="L2X49_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBN4796215.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKYQSVQPTVAKEVSLN"
     gene            40599..>41573
                     /gene="ascG"
                     /locus_tag="L2X49_001094"
     CDS             40599..>41573
                     /gene="ascG"
                     /locus_tag="L2X49_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBN4796216.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKL"
BASE COUNT         9707 a        10831 c        11244 g         9791 t
ORIGIN      
        1 attgtgttcc ccgcgccagc ggggataaac cgacggacaa aatatatatt gatttgcgaa
       61 ttatgtgttc cccgcgccag cggggataaa ccggtaaaga aactgccgac aaatccctgt
      121 tcgttgtgtt ccccgcgcca gcggggataa accgcccgtc accgacgcgc agtggcgcta
      181 ccgtgagtgt tccccgcgcc agcggggata aaccgggatc taacgcgctg taaaaattcc
      241 gtgctttgtg ttccccgcgc cagcggggat aaaccatgcg gattaccggc aaaacatggg
      301 agcaaaccgt gttccccgcg ccagcgggga taaaccgccg aacggctggc gaagcaggtg
      361 gctggcgtag tgttccccgc gccagcgggg ataaaccggt ttaccgcccc gcagaggcgc
      421 tggcagatcc gtgttccccg cgccagcggg gataaaccgg gatgacctgt cgctaaaact
      481 cgccgcgtac agtgttcccc gcgccagcgg ggataaaccg ggcgcactgg atgcgatgat
      541 ggatatcact tagaattccc cgcccctgcg gtagaacacc cagctcccat tttcaaaccc
      601 atcaagacgc cttcgccaac tccttcacca gaggtagcat tatccgcata acgtcacggc
      661 agcgacgttc tattcttcca ggaagtgcct tatcaatatg ctgttgatta tccagtctta
      721 cgtcatgcca gctatttccc gcagggaatg cggctgtttt tgcgcgttgc tgataaccat
      781 ccttattccc aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt
      841 cgaatatttc tgcgtcatta caacacccag tgcctttcgg ataattttta ttcaaacccg
      901 gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta
      961 atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa
     1021 ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct
     1081 tgagtaaatt ctcagcgcct aatttccctt cctcttcgcc gctggtcgcc acaaatcgaa
     1141 taccatactc ggtaggcgta tttttcaggc gttctgccaa ttccagcatg acacctaaac
     1201 ctgcggcgtt atcatccatt ccttgtaacg tcagcccgcc gagattggca tcggcgtcag
     1261 catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt
     1321 tgccttcatg agcggcaatc accgtacttc ccgtcacgtt atgccagctc ttacgattat
     1381 cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt
     1441 gctgaaactg ttggcgaata taatcggcag ataacatttc tgcaggagtt ccggtcatgc
     1501 gtcccggaaa gaaagtagca atatgtcgtg cctgagtatt agcaaaatcg ccaggtttag
     1561 gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt
     1621 ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata
     1681 gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt
     1741 gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca
     1801 ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca
     1861 agaaaatagc ggtattgcaa aggaacggtt atggatcaaa tacgacttac tcacctgcgg
     1921 caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg
     1981 gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg
     2041 ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
     2101 gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat
     2161 aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat
     2221 accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc
     2281 gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct
     2341 ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccagagct gtggcacaac
     2401 tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc
     2461 gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc
     2521 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt
     2581 atcgacctgc aaccgggcga agtgattaaa aaacggatgg tgcgtttccg tacgctgggc
     2641 tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactaccgga gatcatcgaa
     2701 gagatgctgg tttctaccac cagtgaacgt cagggccgcg tgattgaccg cgaccaggcg
     2761 gggtctatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg cacttgcaca
     2821 acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct
     2881 gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgcct
     2941 gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa
     3001 acgtcacggc acccagggcg aaaagctgga tctggcactg ctggtggacg gcctgcaagc
     3061 tgagcgcgaa cagggcatca ccattgacgt ggcttaccgc tatttctcta ccgagaagcg
     3121 taaatttatt atcgccgaca ccccagggca cgagcagtac acccgcaata tggcgactgg
     3181 cgcatcgaca tgtgaactgg cgatcttact gatcgatgcc cgtaaaggcg tgctcgatca
     3241 aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat
     3301 caacaaaatg gatctggtgg attacagtga aaagacgttc acccgtattc gtgaagatta
     3361 tctgaccttt gccgggcagc tgccgggtaa tctggatatc cgctttgtgc cgctctctgc
     3421 actggaaggc gacaacgtgg cttcgcaaag tgaaagtatg gcgtggtaca gcggtccaac
     3481 actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgctc agccaatgcg
     3541 cttcccggtg cagtacgtta atcgcccgaa tctcgatttt cgtggttacg ccggaacgct
     3601 ggcatccggt cgcgtagaag tcgggcaacg agtcaaagtg ctgccctctg gtgtggaatc
     3661 aaacgtcgcc cggatcgtga cttttgatgg cgatcgcgaa gaagcctttg ccggagaagc
     3721 gatcaccctg gtgctgacgg atgagatcga catcagccgt ggcgatctgc tgctggcggc
     3781 agacgaagcg ttaccagctg tgcagagcgc gtcggtggat gtggtatgga tggcggaaca
     3841 gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg
     3901 tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa
     3961 cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga
     4021 tcgttatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc ttagcaatgt
     4081 gaccgtgggc gccggtatgg tgcacgagcc agttagccag gcaactgctg cgccatctga
     4141 atttagtgca ttcgaattgg aattgaatgc cctggttcgc cgccactttc cacactgggg
     4201 cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat
     4261 agccatccgg tcactgtgca acaacgcgag ctacaccacg gtcatcgtgg tgtagtgctg
     4321 tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg
     4381 ttacataaac tcggcgtcag tacgtatctg ctggatggcg ataatgttcg ccacggatta
     4441 tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa
     4501 gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac
     4561 cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt
     4621 gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg
     4681 cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg
     4741 gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat
     4801 ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt
     4861 cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga
     4921 cagccttact gaggatgaag aaaccacatg gtcactgcct ggtgccgtgg tcggttttat
     4981 ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt
     5041 ctttatctac acctggcctt tctttctggc gctgatgccc gtcgcggtag tggtggggat
     5101 tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac
     5161 agtaggcatt atgtttggcg cactgtttat gtggctactg ggctaatttg tactttcccg
     5221 tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg
     5281 gtatacgtaa caatggacaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca
     5341 gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg ccgtttttca gggggcagga
     5401 tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt
     5461 tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg gcacagcaag
     5521 ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca
     5581 atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg
     5641 gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa
     5701 acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg
     5761 tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct
     5821 caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg
     5881 tgaaacgagt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg
     5941 cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg
     6001 caggtctgaa agccgctggc gacgcgcagt gggtattggt gcatgacgcc gctcgtcctt
     6061 gtttgcatca ggatgacctc gcgcgattgt tggcgttgag cgaaaccagc cgcacggggg
     6121 ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca
     6181 ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc
     6241 gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgcgact attaccgacg
     6301 aagcctcggc gctggaatat tgcggattcc atcctcagtt ggtcgaaggc cgtgcggata
     6361 acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca
     6421 tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacatg cctttggcgg
     6481 tgaaggccca attatcattg gtggcgtacg cattccttac gaaagaggat tgctggcgca
     6541 ttctgatggc gacgtggcgc tccatgcgtt gaccgatgca ttgcttggcg cggcggcgct
     6601 gggggatatc ggcaagctgt tcccggatac cgatccggca tttaaaggtg ccgatagccg
     6661 cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt
     6721 cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt
     6781 gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac
     6841 ggaaaaactg ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact
     6901 cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa
     6961 ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt
     7021 gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc
     7081 cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc atgcccgtga agtcagcttc
     7141 gctgggcaaa aagacaaaca tgctgttacg gaacagtggt tatgcgctcg cgtgccgggc
     7201 aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg
     7261 cggcacaagc gcaagctgcg tttaggggcg ctgaaaggaa acgcctttac cctggttctg
     7321 cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt
     7381 gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggggcg
     7441 cagcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg
     7501 tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac
     7561 gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta
     7621 gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga gttgatgata
     7681 accgccgcat tgccaggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa
     7741 caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa
     7801 gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaattg gtgggatgac
     7861 gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg
     7921 gaacttatca acacaacagg tgattatgcg catattgctg agtaatgatg acggggtaca
     7981 tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt
     8041 cgcccccgat cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg
     8101 cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgcgt
     8161 ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa
     8221 cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga
     8281 aggccgtcat ttaggttttc cggcgcttgc cgtctcgctt gacgggcata aacattacga
     8341 cactgccgcg gcggtaatct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac
     8401 cgggcgtatt cttaatatta acgttccgga tttacccttg gatcaaatca aaggtattcg
     8461 cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg
     8521 cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgctg gtccggggac
     8581 cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac
     8641 tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca
     8701 atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat
     8761 gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt
     8821 gaacaaaaag cctgggacaa tatcgctttg ccgataggtc aggggcagac aatttcgcag
     8881 ccatatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg
     8941 gaaattggca ccggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt
     9001 tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt
     9061 gatttacata atgtttcaac ccgtcatggc gatggatggc aaggttggca ggcacgtgcg
     9121 ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg
     9181 cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa
     9241 cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc
     9301 cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta
     9361 ttttgccgca ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca
     9421 cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag
     9481 cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg
     9541 ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc
     9601 aaatactaat tctggtatgt tgattacgcc gccgccgaaa atggggacga cgtctacagc
     9661 gcagcaaccg caaattcagc cggtgcagca gccacaaatt caggctactc aacaaccgca
     9721 aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt
     9781 ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt
     9841 gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct
     9901 tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt
     9961 gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc
    10021 agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc
    10081 gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa
    10141 caagccaact gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga
    10201 gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga
    10261 gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc
    10321 aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa
    10381 cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc
    10441 ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagttaagg cggggcaaaa
    10501 aatagcgacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta
    10561 caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg acggaaccag
    10621 gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat
    10681 acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt
    10741 tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa
    10801 ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag
    10861 attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg
    10921 cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa
    10981 attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac
    11041 ctggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca
    11101 tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact
    11161 attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag
    11221 ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag
    11281 ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc
    11341 ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac
    11401 ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc
    11461 gagctgaacg ccaaacagcg tgaagtgctg gcacgtcgat tcggtttgct ggggtacgaa
    11521 gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag
    11581 attcaggttg aaggcctgcg ccgtttgcgt gaaatcctgc aaacgcaggg gctgaatatc
    11641 gaagcgctgt tccgcgagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg
    11701 gccttttctg tgcacaataa aaggtccgat gcccatcgga cctttttatt aaggtcaaat
    11761 taccgcccat acgcaccagg taattaagaa tccggtaaaa ccgagaatgg tcgttaacac
    11821 tgtccaggtt ttcagaccgt ctgctaccga caaccccaga tatttggtca caatccagaa
    11881 ccctgagtca ttaatatgtg acgcgccaag cccaccaaag caggctgcca gcgtcaccaa
    11941 tacgcactga atcggattca atcccatcac cgcttctgag agtaacccgc cggttgtcag
    12001 gattgctacg gttgctgacc cctgcgatgc acgcagagcc agtgaaataa taaatgcggc
    12061 tggtaacaga ggcaggtcaa tcatttgtag catattggca agggctttgc cgacgcccga
    12121 ttccaccagc actttgccaa atacccctcc agcaccagta accaaaatca ctaccgccgc
    12181 agtgggaagg gctgagccca taatgtcgct ggtgtgttgt aagctccagc cgcgacgtaa
    12241 agccaataac cagaatgcca gcaccagcgc aatcattaga gctaccattg gtgagccgat
    12301 cagctgtagc gtaccaagca ggggatgcga aggcggcatc agtgttgcgg aaaccgtacc
    12361 cgccatgata atcgcgatag gaataacaat tagcgaggtg accagcgcga cgcccggtgg
    12421 atttatttta tcgcttaatt ttgtcgcgcc ttcctcactg gccggagcca gttgcatctg
    12481 ttccagtact tccaccgaca tcgcatattg gtgcttattg attattttcg ctgcaaagta
    12541 gccaacaatc cctacgggaa tagaaatcgc aataccgatg atggttagcc agccgatgtc
    12601 tgcgtggagt aaccccgctg cggcgacagg gcctggatgc ggcggtaccg ccacatgtac
    12661 agttagcatg atcccagcga caggcaggcc aaatttgagt ggcgatattt tggcaacctt
    12721 ggcaaaaccg taaatgattg gcgcaagaat aataaagccg acatcaaaga agacgggaat
    12781 accgaggaag aacgctgcca gagtcagcgc agcgatagtt cgtttgtcac ctaacttgcg
    12841 actgaaataa ttagccagtg actctgcacc accagagtgt tcgatcatac gccccagcat
    12901 agcgcccaga ccaataataa tagtgacgga accaagcaca ccgcccatcc cggcgatcat
    12961 cactttaccc acttcgcccg ccggtatacc cgccgcaagt gcgactaaca ggctgacgag
    13021 gagcagagca acgaatggtt gtacctttgc cttgatgacc agcagcaaca gcatgattac
    13081 gccagttaac gcaatgcata acaatgtaat tgtggacatg ggaaaccctg tctgaaagtt
    13141 atagttaacc taccccatcc gtagatgggg ggatgtatgg gtacgttgta attagggatt
    13201 taacgaatta gcgccaggcg tcaaaccagc caagcccttc ttcggtgagg ccacgaggtt
    13261 tatattcaca accgatccag ccctgatatc ccacctcatc gaacaggcgg aacagccacg
    13321 gatagttgat ttctccatcg tccggttcat gtcgatcagg tagtccggca atttgtacgt
    13381 gcgcatattt cccggcgtag tcgcggatta aatgcgtcag gttgccatct actttttgcg
    13441 catgaaaagt atctagttga ataaacacgt tatctcgcgc aacctcttca acaatagcca
    13501 gtgcctgata ctggctggag aagagataat gaggcttaac gtcggggctg agtgcttcaa
    13561 ctaatattcg cttgccgtgt agcgcaaagc ggtcggcagc gtagcggaga ttatcgataa
    13621 atactgcccg gtaccgttca gcgtcttcgc cagcgggcac gacgcctgcc atcacatgga
    13681 cttgttcaca attgagcgcc aatgcatatt ccagtgccag gtcgatgtct gcgtgtgctt
    13741 cgtgctcacg tccgggaagg gcggataatc cccattcccc cgcattaata tctccgggag
    13801 cggtattgaa cagcgccagt gtcagatggt tttgctccag ttgcttttgg atttgcaggg
    13861 tggagtagtc atagggaaac agaaattcca cagcatcgaa cccggctttt cgcgctgcgg
    13921 cgaagcgttc aataaaaggc acttcggtga acatcatgga taaattagct gcaaaacgag
    13981 gcattgcatt aactccttaa ttccgcaatt tcacctgcgg tcagataacg gatcgggcgg
    14041 tcaccgagaa taaaaatcag ctttgccgtt tcctccagct cttccatatt gttggcggct
    14101 tcttgcaggc tttcaccgca aaccactggg ccatgatttg ccagtaaaaa agcctgattg
    14161 tctgctgcca gttccgccag atcctgtgcg atgcgtttat cgcccggtcg gtaataaggc
    14221 accagcggga catttcccat ccgcatcacc acgtatggtg tgaacggacg aataacgttg
    14281 ctgctgtcca gcccttgcag gcaggaaagc gccgtcgacc atgtgctgtg caaatgcacc
    14341 accgctttac agcgcggatt gttgcgatac agcgccagat gaaagagcac ctctttcgag
    14401 ggtttgtcac cacttaacca ttcgccatcc gcggcgactt tggaaagccg ctgcggatcg
    14461 agattgccca ggcatgaacc tgtcggtgtc gccagtaaat tcccgtcagg taaaagcagc
    14521 gacagattgc cagccgaacc ggttgcatag ccgcgctgaa agaatgaact ggcaatccgc
    14581 gtcatctcct ctcgcaaaga ctgctctact tttgcgaaat cgctcatgat aaaaactctc
    14641 tttgggctcg tgaaaaaaag gcttcatcac cgaagttgcc agatttaagg gcgagtgaga
    14701 caggcttatc cagtgcgttt acccacggca cgccggggga aatggttggg ccaatatgaa
    14761 acccttttat gcccaggctc tgtgtgacta cgccggaggt ctcaccgcct gcgacaataa
    14821 agcgtgtcac gccttccgct gctaaccgcg ccgctagttg agaaaacaga gtttctactg
    14881 cctgactggc tttttgtgca ccgtattgct gttgaattgc tgccaatgcg tcagtgctgg
    14941 cggtggcaaa aaccagtgga gcaagtacac tttcctggcc cagaacccac tctgccagtt
    15001 cgtgtgcata agcggccaga gtttcggttg agaggcagcg tgccacatca acttcacggg
    15061 ctggtgcaat ttgacggtaa tgtgccacct ggcggttggt catttgagag catgaaccgg
    15121 agagcactac gccgcgccca gcgagcggat gccctgcttc gcgagcctgg ttaccgtttt
    15181 cttgcgccca ctgccgggcc aggccaatcg ccagaccaga accgcccgtt accagtgggg
    15241 catcgcgcaa ggcttctccc tgaatttcca gatggtgttc ggtcagcgca tcaagcaccg
    15301 cgtagcggta gccctcttgc tgtaagcgag ccagctcttg acgaacggca tccacacctt
    15361 gttcgaaaac atgtgccgaa acgacgccgc agcgccctgt ggattgcgct tcaaccagac
    15421 ggggaagata gctgtcggtc atgggattta ccgggtgatg gcgcatcccg gattcggcca
    15481 gcagttgatt cattacgaac aaatacccct gataaaccgt acgtccgttg accggcaggg
    15541 ccggagagaa gaccgtaaac ggcgtgtcga gagcatccat taatgcatcg gtaaccgggc
    15601 cgatattacc tttcgccgta ctgtcgaaag tagagcagta tttgaaatag atctgtttgc
    15661 aaccttgctg ttgcaaccag ctcagagccg ccagcgattg ctgtgtggct tcaaccaccg
    15721 gacaggagcg cgttttcagg ctgatcacca gtgcgtcgat tgcttccggc attttacctg
    15781 ttggaacacc gttaatttgt accgttggta gaccgttttc caccagaaaa ctggcgatat
    15841 ccgtcgcgcc ggtaaaatca tcggcgataa cgccaatctt gatcatgatt tcgctcccgg
    15901 tagagtgatg ccagagaaaa tcttgataac tgcgctatcg tcttctttcc cgtaacctgc
    15961 gttactggcg ctggtgaaca tattcaatgc tgttgaggcc aatggcagcg ggaagtgcag
    16021 ggctttggct gtatcggcaa ccagaccaag atccttaaca aaaatatcga cggctgaatg
    16081 cggggtgtaa tcgccatcca ccacatgacg catccggttt tcgaacatcc aggaatttcc
    16141 ggcggcattg gtcacgacgt catacatcac atccagcggg atccccgcac gggctgcaag
    16201 tgccatcgct tcggctccgg cagcaatatg tacgcccgct aacaactggt gaataatttt
    16261 tacggtcgaa cctagtcccg gttctgcacc tatgcgataa acttttccgg caacggcttc
    16321 cagcacgggt gccagtcgtt caaaggcaat atcgctaccg gaggccatga cagtcatttc
    16381 accgttagcg gcttttactg caccaccaga aactggcgca tccagcattt ccagatcgaa
    16441 tccagccaga gcggtagcaa tttcttgcgc atcagcacta gcgatagtgg aagaaaccat
    16501 tactgccgta ccgggtttca gatgttgtgc aacgcctgtt tcaccaaaca gcacctgttt
    16561 aacctgggcc gcattgacca ccagcaccag cagagcgtcc agtttttcgg caaacgtcgc
    16621 ggcgttatca gaaaccccgc aagcacctgc ctctttcaac gtagcgcagg cattgctgtt
    16681 caggtctgcg ccccaggtag aaagacctgc gcggacacat gacagtgctg ctcccattcc
    16741 cattgaccct aagccaacga taccgacatg aaactcagat cccgttttca tctgctctcc
    16801 ttgttaattt aagtgatatt ttgtttgata ttgtgaatat aagcgctgga agataacgat
    16861 atggtgagct gattcacata aattaacatt gtgtgttatt ttatgtgaac taagcgttag
    16921 ttgcgccgcg cacgtttcgc aggcaaatag cgtagaatgt cagcaggaca aaggaaggag
    16981 caaaagttga tacccgtaga gcgtcgccaa atcatccttg agatggtagc tgaaaaaggc
    17041 attgtcagta ttgctgaact aacggacaga atgaatgtgt cacatatgac cattcgtcgg
    17101 gatttacaaa aactggagca gcagggagcc gttgtgctgg tgtccggagg cgtccagtct
    17161 ccgggacgcg tggcgcatga accttctcat caggtaaaaa ctgcgctggc aatgacgcaa
    17221 aaagcggcta ttggcaagct ggcggcaagt cttgttcagc cgggaagctg tatctatctg
    17281 gatgcgggaa cgaccacgtt agcgatagca cagcatctga ttcacatgga gtcactgact
    17341 gtggtcacaa acgatttcgt tattgcgaac tacttgctcg acaacagtaa ttgcacaatt
    17401 attcacactg gcggtgcagt gtgtcgggaa aaccgttcct gtgtcgggga agccgctgcg
    17461 accatgctgc gcagcctgat gattgatcag gcttttattt ctgcatcgtc atggagtgtg
    17521 cgggggattt ctacgccagc ggaagataaa gtcacggtga aacgggcgat tgccagtgcc
    17581 agccgccagc gagttttggt ctgtgatgcg acgaaatatg gtcaggtggc gacatggctg
    17641 gcgttaccat taagcgagtt tgatcagatt atcacagacg acggtttgcc ggagagtgcc
    17701 agtcgcgcgc tggcgaagca ggatctctct ttgctggtag cgaaaaatga ataatggcct
    17761 gcaataacat ttggttactc atgcttcaca gaagaagcat gagactactt tattttataa
    17821 aatgacagcc gcccgctttt cggcgtgccg gtatcaatat aaatctggtt agcgaacgtc
    17881 tgaatgttat caaacatcat atgtccaaat ataaaataat cagcgccgtt tatttgttgt
    17941 aactcgccat taagcgattt ctgcacacga tcaacaggcc agagtaattc gctctccgct
    18001 atttctttac caaagagata ttcactcccc ggataatctg catgtgcgat gacatatttt
    18061 atgttgtcgt tagtgatttc aataatatgt ggaaggtgat ggaatttcag caacagatct
    18121 attgcctctt gttgctctga atcatttaaa tcgaaaaacc agtcaccacc gctggcaagc
    18181 cacatattgc catcgccagt ttcgaatgca tccaacgcca tcgcttcgtg gttgccttta
    18241 accgacgtaa accagggttg gtttagcagg cgcagcacgt taagactctt cggcccacga
    18301 tcaatattat cgcctgtgga aataagtaag tcggtttcag ggtaaaaaga gagttgatgt
    18361 aaacgggatt gtaataactg atattcacca tgaatatcac caacgaccca tatatggcga
    18421 tagtgatggg cattgatttt ttgatagcgt gtagatggca tggttttacc ctgtaaaata
    18481 agctttccta ttatacaggg tatttttatt tgattcgtca gttgtcgtta atattcccga
    18541 tagcaaaaga ctatcgggaa ttgttattac accagactct tcaagcgata aatccactcc
    18601 agcgcctgac gcggagtcag tgaatccggg tcgagatttt ccagtgcttc gaccgcaggc
    18661 gaagtttctt ctggcactga cagcaaagac atctgcgtac catccacttg cgtcgcggcg
    18721 gcgttcggcg aaatgctttc cagctcacgc agtttttgcc gtgcgcgctt aatcacctct
    18781 tttggcacgc ccgccagagc tgcaaccgcc aggccgtagc ttttgctcgc cgcgccatcc
    18841 tgcacgctat gcataaaggc aatggtgtcg ccgtgctcca gcgcatcgag atgcacgttg
    18901 gcgacgcctt ccattttctc cggtaactgg gtcagctcga aatagtgggt agcaaataac
    18961 gtcaatgcct taatcttatt cgccagattt tccgcgcacg cccacgccag cgacagacca
    19021 tcgtaggtgg acgttccacg cccgatctca tccattaaca ccagactgta ttcggtggcg
    19081 ttatgtaaaa tattggcggt ttcagtcatc tccaccataa aggttgagcg cccggaagcc
    19141 aggtcatctg ccgcgcctac gcgggtaaag atgcgatcga taggtccaat ctcgactttt
    19201 tgtgccggta catagctgcc gatgtaggcc atcagcgcaa tcagtgcggt ctggcgcata
    19261 taggtacttt taccgcccat gttcgggccg gtaataatca acatacggcg ctgcggcgac
    19321 agattcagcg ggttagcgat aaatggctca ttcagtactt gttcaactac cggatggcga
    19381 ccttcggtaa tgcgaatgcc cggtttatca atgaaggtcg ggcaggtgta gttcagggta
    19441 taggcccgtt ccgccaggtt aaccagcacg tcgagttccg ccagcgcgct cgcgctctgt
    19501 tgcaacgctt ccagatgcgg caacagcagg tcgaacagct cttcataaag ctgtttttcc
    19561 agtgccagtg ctttgccttt tgaggtgaga actttatctt cgtactcttt tagctctgga
    19621 atgatgtagc gctcggcgtt tttcagcgtc tggcgacgca tgtagttgat gggtgccaga
    19681 tggctttgcc cacggctgat ttgaatgtag tagccgtgca ccgcattaaa gccaactttc
    19741 agcgtgtcca ggccggtacg ttcacgctcg cggacttcca gacgctccag ataatcggtc
    19801 gcgccgtcag ccagcgcgcg ccactcatcc agctcttcgt tatagcccga tgcgataaca
    19861 ccaccgtcgc gtaccagcac cggcggtgtg tcgatgattg ctcgctccag cagatcgcgc
    19921 agctcggcaa actcgcccat cttctcacgt agcgcctgta ccggtgcact atcgacagtt
    19981 tctaactgcg cacgcagctc cggcagttgc tggaaagcgt ggcgcatacg ggccagatcg
    20041 cgtgggcgag cagttcgtaa agccagacgt gccagaatac gttccaggtc gccgacctga
    20101 cgcagtaccg gctgcaactc ggcggtgaaa tcctgcaatg cgccaatagt ttgctggcgc
    20161 tcaagcaaca cgcgggtatc gcgcactggc atatgcagcc agcgtttcag catacgacta
    20221 cccatcggcg tgacggtgca gtcgagcaca gaagccagcg tattttccgc accgcccgcc
    20281 aggttctgag taatttccag gttacgacgt gtcgcggcat ccataatgat gctgtcctgc
    20341 tcacgttcca tggtgataga acgaatatgc ggcagggtcg tgcgttgggt atctttcgca
    20401 tactgcaaca gacaaccggc agcacaaagt ccgcgcggcg cgttctcgac gccaaaaccg
    20461 accagatcgc gggtgccaaa ttgcagattc aactgctggc gcgcggtgtc gatttcaaac
    20521 tcccacagcg ggcgacggcg caggccgcga cggccttcaa tcagcgacat ctcggcgaaa
    20581 tcttctgcat acagcagttc cgccggatta gtgcgttgca gctctgccgc catcgtttcg
    20641 cggtcggccg gttcgctcag acgaaaacgc ccggagctga tatccagcgt cgcgtagccg
    20701 aaacctttgc tgtcctgcca gatagccgcc agcaggttgt cctgacgctc ctgtaacagg
    20761 gcttcatcgc tgatggtgcc tggcgtaacg atacgcacaa ctttgcgctc aaccggacct
    20821 ttgctggtcg ccggatcgcc aatttgttcg cagatggcaa cggactctcc ctgattcacc
    20881 agtttggcga gatagttttc caccgcatgg tagggaatcc ccgccatcgg gatcggctct
    20941 cccgccgaag caccgcgttt ggtcagtgaa atatccagca gttgcgacgc gcgttttgcg
    21001 tcgtcataaa acagttcata aaaatcaccc atccggtaaa acagcaggat ctcgggatgc
    21061 tgggctttca gcctgagata ctgctgcatc atgggcgtat gggcgtcgaa attttctatt
    21121 gcactcatgg ggttatgtcc ggttccctga tattaaatgg ggtgtgatgg tttttatttt
    21181 cgttgtaatc acataaggcg cacatgatac cggagttaat catttcaggg gaagaagcga
    21241 acggcgagtt cttgatgctg gttccgggaa agatgctgtt ttgcagcttt tgcgtgatgc
    21301 ttatgaagca agtacgcaaa attgcgtcgg ttgaataaac ctcctccttc agagccatag
    21361 tcccctttca aattaagttg taatataaat acaccttaag agtatggagg cagcatgtcc
    21421 ggcaagcgta tctctcgtga aaaactgacg attaaaaaaa tgatcgatct ttatcaagcg
    21481 aaatgcccgc aggcgtcagc ggagccggag cattacgagg cgttgtttgt ttacgcgcaa
    21541 aagcggctgg ataaatgtgt gttcggcgaa gagaaaccag cctgtaagca gtgtccggtg
    21601 cactgttacc agccagcaaa gcgtgaagag atgaaacaga ttatgcgttg ggccgggccg
    21661 cgaatgctgt ggcgtcatcc gatcttaacc gtgcgtcatc tcatcgatga caagcgtccg
    21721 gtgccagaac tgccggaaaa atatcgtccg aaaaaaactc gtgaatagta tgccggacag
    21781 gcagatctgt ccggcaattt gcagttaaat caatgccgat ttatcaattc caagccgctt
    21841 catccgtgac agcaaggtcg tgcgtttcag ccccagacgt tgcgcagcgc ctttaggccc
    21901 ggcaaccacg ccgttggttt ctttcagcac gcgaacaatc aactgatatt catcttcgcc
    21961 ctcctgggcg acaaccgttg cggcaggcgg cgtttcaggt tccggtaaaa caatatctgg
    22021 caatgacagc tgcagcacgt taccgcgtgt tagcaatacc gcgcgctcaa tgacgttttc
    22081 cagttcgcgt acgttacccg gccactccat gttgctcaag gtgcgcagcg tctcggcagg
    22141 aatgctgtcg atattgcgcc ccagacgacg ggcaattttg aaggtaaagg ctttcgccag
    22201 cagcggaata tcttccggac gctcgcgtag tggcggcagg tgaatcggga atacgttcag
    22261 gcggtaatag agatcgctac ggaactcacg gtcggcgacc atttttttca gatcgcggtt
    22321 agtcgcggcg attagacgca cgtccgtctg aatgattttg ttgctgccga gacgttcaaa
    22381 ctcctgttcc tgcaatacac gcagcaactt cggctgtaac tccagtggca tatcgcccac
    22441 ttcgtcgagg aacagggagc ttttatccgc cagttcaaaa cgaccaatgc gctgggcgct
    22501 ggcaccggta aaagccccac gctcatgacc aaacagatcg ctttccagca atccggcagg
    22561 catcgccgcg cagttcattt tgaccatgcg gcgattatta cgcccactga gattatggat
    22621 cgcacgggca atcagctctt tacccgtgcc agtttcaccg aggatcagca cggtactgtc
    22681 actttgcgcc accatttcaa cttgtttaag cacgctgtac atggcttcgc tgcggccaat
    22741 aatctcgcca aattcactat caacattgtt gagctgctcg gtcagggcga ggttttcatc
    22801 aaccagccgt tctttcagac gatggatttc ctgataggcg agggcgttat cgacagcgat
    22861 tgccacacgt tcggcaatct ggcgcagtaa attcagattg gtagtggtaa acactttctc
    22921 ttcgcattgc gccagtttca gcacgcccag catggtgtcg ccagacatca gcggtaacag
    22981 gcacaaggtt tgaatctggt tgccccaggt gtcgaacaac atgcgttcat agggggctaa
    23041 atcgtcccgc tcgtggagat tgatcagcag catctcttta cttttgaaca cgcgttcggt
    23101 gagggttccg gcttcatcga cttcgctctg ttcgtgggcg ggatgttgtt tatcaagata
    23161 gtgagtggag tagatgttga gtttgttttt acggtggctg cgtaagacaa tgctgatatc
    23221 gtcaatgtcg aaatagtaat ggatttcttt ggcgacttcg ctgaccagtt cgtccatatc
    23281 caggcgggaa agcaccgcgt tggtaatggc aaccaggatg cggaagttat cgcgctcccg
    23341 gcataacaac tcatagtcga cattgttgac gacgcggctc tggatttgtt cggtgacgac
    23401 agaaacgatc tgcgtaaatg tttgcagacg attgaactct ttttcgctcc aggggcgatc
    23461 gtcataacga ataaattcac agccaccaaa aatatgccct tccgccgcca gtggcatcag
    23521 gcaatagtga ccaaatttgg ggtatagccc acctgcggcc agctgcggcc aggtttcaca
    23581 aaattcttcg taactgcaat gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc
    23641 gtgtgccaga acagtttcgt cttcatattt aatgggggtg tctttttcac gcgatgcgta
    23701 ataagacgca cgttgagtct gcgcttgcca caacacaata gccgcgttgt cggcgagcgc
    23761 agaacgcttt accagttgcg aaagagcctc acacagcgag gccagatcgg gctgctgcaa
    23821 tagtgtccga gtgatgtcga acaacccttg ctgtccgaga tcactcatcg gtgtatatga
    23881 cattttgttc ttccaggaaa gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag
    23941 gagatttaga attttattag catatacgcg gaagcggttc ggcgtgtggt aaatcgaggg
    24001 ttcgtttcac gccatacaga ccggcaagac gaacaccttt acgttccacc acttcaccaa
    24061 tcagcgccgc gtctttcccc agtggatggg aatgtaacgc tgccagcact tgctctgccg
    24121 cgttgcgttc aacagctatt actagtttgc cttcgttggc aaagttcagg gcgtccagtc
    24181 ccagcaattc gcaaacgcca cgcacggcag gtttaacagg cagtgccgct tctgaaagtt
    24241 caataccaca accgcaggct gccgcgaact catgaaccac cgcgtttaca ccaccacggg
    24301 tggcatcacg cagcgctttc acgccgggaa tgtcacgcag cgtctgaata agcggcgtca
    24361 gcaccgcgca gtcgctgacc agttcgccat ccagccccag ctgctcacgc aggttaagga
    24421 tagtcgcccc gtggtcgccg agtgtaccgc tcaccagcaa tacatcgcct gcggttagcg
    24481 tctgtgcgcc ccagtgaata ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca
    24541 gtttatctac cgcgccgcgc tgcaccactt tagtatcgcc agtaacgatg gcaatgcctg
    24601 ccgtgcgggc ggtttctgcc atgctggtca ctacggcttt cagtgtctcc atcggcaatc
    24661 cttcttcgag gataaagcca caggagagat agcgcggaat agcgccactg accgcaacgt
    24721 cattcgcggt gccgcaaatc gccagcttgc cgatattacc gccaggaaag aacagcgggt
    24781 caatgacgta actgtcggtg gagaacgcca gacgatcgcc ttctgctacc agctgcgcca
    24841 gatcaagacg tgcctggtct tcctgctctg ccagccacgg gttggcaaag gcttccataa
    24901 acaggctgtt gattaattgc tgcatcgcct ggccgccgct gccgtgggcg agttggatat
    24961 tattcacgct tcattctcct gctgacgata ctgataccac gcggcgcacg ctccttcgga
    25021 ggagaccatc agtgcaccaa atgcggtttg aggattacag gtgttaccaa acagcgggca
    25081 ttgatgcggc ttacatttgc ccgttaatac ttcaccacaa cgtgcgcgcg ggtcatcgca
    25141 gacctgctgc ggtgccgggc ggaaatgtgc ttcggcatcg aatcgttgat agtccggcgt
    25201 caggtgtaca ccagaagatt caatcacgcc taagccgcgc cattcgctgt cgccgttgac
    25261 gcagaagaca tccgcaatcg cctgttgcgc cagcaggtta ccggcatccg gcactacccg
    25321 ccgatactga ttttctacct tgctatgagc agctattttc tgctccacca gcatgaccac
    25381 gccttgtagt agatcaaggg gttcgaatcc agcaaccacc agcggacgat gaaaatcgct
    25441 ggcgataaaa ttataggcgt tggtgccgat aaccatactg acgtgacctg gcgcgaggaa
    25501 cgcatcgata ccgttatccg gctgttccag caaactgcgc aacgtcggga taagcgtaat
    25561 gtgctggcag aagaagtaaa aattctgcac atcacgcgct ttcgcctgtt gcagagtgat
    25621 ggcggtggtc ggcatggtgg tttcaaaacc taagccgaag aacaccactt tgcgggttgg
    25681 attctcctgc gccagtttca acgcatccat tggcgagtaa acgatgcgaa catcggcacc
    25741 gcgtgctttt gcctgcaaca gcgatccctg tttccccggc acgcgcatgg cgtcgccaaa
    25801 ggtacagaag atgacttccg gatggctggc aatctccacg caggtgtcga ttctacccat
    25861 cggcagtacg cacaccgggc aacccggacc gtggataaac tcaacgtttt caggcagtaa
    25921 ctggtcgagg ccgaatttaa aaatggcgtg ggtatgacca ccgcacactt ccataatccg
    25981 cagagggcgt tcggctgtgt aagagagatg tgaagcacgt tcgcgcagat gctcaattaa
    26041 ctgcatcacc tgttccggcg cgcgatattc atcaacaaaa cgcattattt ttcctcgcca
    26101 tacaacagcg cgccgacatc cggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg
    26161 tcgcgtgctt cggcttcatt aattacgctc atggcaaagc caacgtgtac cagtacccac
    26221 tggcccacgc gcggctgacc gttttcatcg cagctgccga ctaacgttaa atcgacatcg
    26281 cgctgaatgc cgcagacgtc gactttcgcc tggttgccgt caatggtgcg gatctggccg
    26341 ggaacgccta tgcacatcgc tgtgtctcca gccagttcag ccactggtcc atcccttcgc
    26401 cgctggtggc ggaaataagg atgatttcaa tttctggatt gacttcgcgg gcgcaggcga
    26461 tgcacttctc aacgtcaaag ttgagatacg gcagcaggtc aactttgttg agcagcatca
    26521 gcgaggcggc ggcaaacata tgcggatatt tcagcggttt gtcttcacct tcggtaacgg
    26581 aaagcaccgc cactttgtgt ttttcaccga gatcgaagct ggccgggcat acgaggttgc
    26641 caacgttttc gataaacaga ataccgttat cgtccagtgg caggcgcggt gcggcttcgg
    26701 caatcatctg tgcgtcaaga tggcagcctt taccggtatt cacctgaatc gctggtgtgc
    26761 cggtagcgcg aatgcgtgcg gcatcgttca cggtttgctg atcgccttca ataactgcgc
    26821 acggaacgct gtctttcagg cgcattaggg tttccgtcag cagggtggtt ttaccggaac
    26881 cagggctgga aaccaggttg agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc
    26941 gttcagccag acggttattt ttgtccagca cgtcaatttc gacttccagc atccgacgct
    27001 ggctcatgcc cggcgcgtga gtgcctgctt cgccatgacc gtaatgcagg tcgccttctt
    27061 cagtctggct gggggtaaat tcaggcgctt taatgccggt gattttcatc ttcgggcgtg
    27121 ccgccggggc aaatggcgcg ctacgaaacg cggaatgagg gttatgttca tcaccctcga
    27181 tatacaggtt gccttcaccg caaccgcatg ttgtacacat cgctcactcc tggtctattt
    27241 ctatccgccg aatctgtaaa ccgtcgtctg ccacaatctg tagcatgtca ccatgacact
    27301 gtggacagcg gcggacgcgc tgggtcagta gcgtcacata ctgttggcat gtttcacacc
    27361 agcattcggc ctcttgttct tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc
    27421 aaaccagatc aaaacaaaag gcaagagagc tggtttcgac acaagaaaat gcgccaattt
    27481 tgagccagac cccagttacg cgttttgcgc cgtgttttgc ggcctgctgt tcgatcaatt
    27541 ccagtgcccg ttggcagagg gttatttcgt gcatatcgcc tcccattaac tatggccagc
    27601 tacaagcaat aattgtgcca gtattgatta tccctgcggt gaataatgtc gatgatgtcg
    27661 aaatgacacg tcgacacggc gacgaaattc atctttagct taaaaatcgt tttaataaca
    27721 atgaattaaa agttggcaca gaaaatgctt aaagctggca tctctgttaa acgggtaacc
    27781 tgacaatgac tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc
    27841 gcctacagga ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga
    27901 aagcgcgcct gcatcacgcc atgagctgcg cgccggacaa agaactctgt ttcgtccttt
    27961 ttgaacattt tcgcatttac gtcaccctgg cggatggctt taacagccac accatcgagt
    28021 attacgtcga aacaaaagat ggcgaagaca aacagcggat tgcgcaggcg caactgagca
    28081 ttgacggcat gattgatggc aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact
    28141 atctcgaaaa aatcgctggc gtttacgaca gcttatacac cgctattgaa aacaatgtgc
    28201 cggtgaattt aagccaactg gtaaagggac aaagcccggc agcatgagct gaggctttgc
    28261 ccgttttgca ggcgttacgc ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac
    28321 atttcatcgg catgttttcg tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt
    28381 tttgtaattg ctgactccac gctctgtatc ggctgccaca cttgtgaggc cgcctgttca
    28441 gagacgcatc gccagcacgg cctgcaatca atgccgcgcc tgagagtgat gctgaatgaa
    28501 aaagaatctg cgccgcagct ctgtcaccac tgtgaagatg caccctgcgc ggtggtctgc
    28561 ccggttaacg ccatcacccg cgttgatggg gccgtgcagt tgaatgaaag cctgtgcgta
    28621 agctgcaagc tgtgcggcat cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt
    28681 ccgctggata ttccggcaaa cgccaatacc ccgaaagcgc caccggcacc gcctgctccg
    28741 gcgcgtgtca gcacattgct tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt
    28801 gacctttgta gctttgatga acaaggtccg gcctgcgtgc ggatgtgccc gactaaagcc
    28861 ctgcatctgg tggataacac tgatatcgcc cgcgtcagca aacgtaagcg tgagctgact
    28921 tttaacacgg actttggcga tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa
    28981 tgagcgcaat ttccctgatc aatagcggcg tggcgtggtt tgtcgccgcc gctgttctgg
    29041 catttctctt ttcttttcaa aaagcgttaa gtggctggat agctggaatt ggcggcgcgg
    29101 ttggtagtct gtatacggca gccgcgggct tcactgtact gactggcgcg gttggcgtga
    29161 gcggtgcgct gtcgctggta agctacgatg tgcaaatctc tccgcttaac gcgatttggc
    29221 tgattacgct cggtctgtgc ggtctgtttg tcagcctcta caacattgac tggcatcgcc
    29281 acgcgcaggt gaagtgcaac ggcttgcaga tcaatatgtt gatggctgcc gccgtctgcg
    29341 ccgtcattgc cagcaacctc ggcatgttcg tggtaatggc cgaaatcatg gccctgtgcg
    29401 cggtgttcct caccagcaac agcaaagagg gcaaactgtg gtttgcgctg gggcgtcttg
    29461 gcactctgct gctggcgatt gcttgctggc tgctgtggca gcgttatggc acgctggatc
    29521 tgcgcctgct ggatatgcgt atgcaacagc tgccgctcgg ttccgatatc tggctgctcg
    29581 gtgtgattgg ctttggcctg ctggccggga ttattccgct gcacggctgg gtgccgcagg
    29641 cacatgctaa cgcctctgcg ccagctgccg cgttgttttc tacggtagtc atgaaaattg
    29701 gcctgctggg cattttaacc ctgtcactgc tgggcggtaa tgcaccgctg tggtggggga
    29761 tcgcgctgct ggtgctcggc atgatcaccg cgttcgtcgg tggtctgtat gcgttgatgg
    29821 aacacaacat ccagcgcctg ctggcttacc acaccctgga aaatatcggc attatcctgc
    29881 tggggctggg cgctggcgta acgggtatcg cgctcgaaca accggcactg attgctcttg
    29941 gcctggtcgg tggtctgtac catctgctta accatagcct gttcaaaagc gtactgttcc
    30001 tcggagcggg gagcgtctgg ttccgtaccg gtcatcgcga tatcgaaaaa ctcggtggta
    30061 ttggcaagaa aatgccggtt atctccatcg ccatgttagt cgggctgatg gcaatggctg
    30121 cgctgccgcc gctgaatggt tttgccgggg aatgggttat ctatcaatcc ttcttcaaac
    30181 tgagcaatag tggcgcgttt gttgcccgtc tgctggggcc gctgctcgcc gtgggtctgg
    30241 caattaccgg tgcgctggcg gtgatgtgta tggcgaaagt ctatggcgtc acgttcctcg
    30301 gcgcgccgcg taccaaagaa gccgaaaacg ccacctgtgc accgctcctg atgagcgtaa
    30361 gcgtagtggc actggcgatt tgctgcgtaa ttggcggtgt tgctgcgccg tggctgctgc
    30421 cgatgctgtc tgctgctgta cctctgccgc tggagcctgc taacaccacc gtttctcaac
    30481 cgatgatcac gttgctgctg attgcctgcc cgctgctgcc attcatcatt atggcgattt
    30541 gcaaaggcga tcgtttgcca tcgcgttccc gcggcgcggc ctgggtgtgc ggctacgacc
    30601 acgaaaaatc aatggtgatt accgctcacg gttttgccat gccggtgaaa caggcgcttg
    30661 cgccagtgct gaaactgcgc aaatggctga atccggtgtc gctggtgccg ggctggcagt
    30721 gcgaaggtag tgcgttgctg ttccgccgga tggcgctggt tgaactggcg gtactggtgg
    30781 tgattattgt ttcacgagga gcctgagaat gagtgtttta tatccgttaa ttcaggcgct
    30841 ggtgttattt gccgttgcgc cgctgctctc cggtattacc cgcgtggcgc gcgcccgtct
    30901 gcataaccgt cgtgggccgg gtgtgttgca ggagtatcgt gacattttca aactgctggg
    30961 ccgccagagc gttggcccgg atgcctccgg ctgggtgttc cgcctgacgc cgtatgtgat
    31021 ggtgggcgtc atgctgacta tcgctactgc gctgccggtg gtgaccgtcg gttctccgct
    31081 gccgcaactg ggtgatttaa tcaccttact gtatctcttt gccatcgcgc gtttcttctt
    31141 tgccatttct ggtctggata ccggtagccc gtttaccgct atcggcgcga gccgtgaagc
    31201 gatgcttggc gtgctggtcg aaccgatgct gctgcttggt ctgtgggttg ccgcacaggt
    31261 tgccggttcc accaacatca gcaacatcac cgacaccgtt tatcactggc cgctgagcca
    31321 gagcatcccg ctggtgctgg cgctttgtgc ctgtgcgttc gccactttta tcgaaatggg
    31381 caaactgccg ttcgacctgg cggaagccga gcaggagttg caggaaggtc cgctctccga
    31441 atacagcggt agcggctttg gcgtcatgaa atggggtatc agcctgaaac agctggtggt
    31501 gttgcagatg ttcgtcgggg tgtttattcc gtggggacaa atggaaacct tcaccgccgg
    31561 tggactgctg ctggcgctgg tgattgccat cgtcaaactg gtggtcggcg tcctggttat
    31621 cgcgctgttc gaaaacagca tggcccgtct gcgtcttgat attactccgc gcattacctg
    31681 ggctgggttt ggctttgcat ttttagcgtt cgtctccttg ctggcggcgt gattaaagag
    31741 agtttgagca tgtctgaaga aaaattaggt caacattatc tcgccgcgct gaatgaggca
    31801 tttccgggcg tcgtgctgga ccacgcctgg cagaccaaag atcagctgac tgtcaccgta
    31861 aaggtgaact acctgccgga agtggtggag tttctttact acaaacaggg tggctggctg
    31921 tcggtgctgt ttggtaacga cgaacgcaaa ctgaatggtc attacgccgt ttattacgtg
    31981 ctgtcgatgg agaagggcac taagtgttgg gtaacggttc gcgtcgaagt tgacgccaac
    32041 aaaccggagt atccgtccgt gacgccgcgc gttccggcgg ctgtgtgggg cgagcgcgaa
    32101 gtgcgcgata tgtacggttt gattccggtt ggtctgccgg atgaacgtcg tctggtgctg
    32161 ccggatgact ggccggatga actttatccg ctgcgtaaag acagcatgga ttatcgtcag
    32221 cgtccggcgc cgaccaccga tgctgaaacc tacgagttca tcaatgaact gggtgacaag
    32281 aaaaacaacg tcgtgccgat tggtccgttg cacgtcactt ccgacgaacc gggtcacttc
    32341 cgtctgttcg tcgatggcga aaatattatc gacgccgact accgcctgtt ctacgtccat
    32401 cgcggcatgg aaaaactggc ggaaacccgt atggggtaca acgaagtgac cttcctctct
    32461 gaccgtgtgt gcggcatctg cggctttgct cacagcaccg cctacaccac gtcggtggaa
    32521 aacgcgatgg gtattcaggt gccagaacgt gcgcagatga tccgcgccat tctgctggag
    32581 gtggaacgcc tgcactcgca tctgctcaac ctcggcctgg cctgtcactt tactggcttc
    32641 gactccggct ttatgcagtt cttccgcgtg cgtgaaacct ccatgaaaat ggcagagatc
    32701 cttaccggtg cgcgtaaaac ctacggcctg aacctgatcg gcgggattcg tcgcgatctg
    32761 ctgaaagatg acatgatcca gacccgccag ctggcgcaac aaatgcgtcg tgaagtgcag
    32821 gagctggtgg atgtgctgct gagtacgccg aacatggaac agcgcactgt cggcattggt
    32881 cgtctggacc cggaaatcgc ccgcgatttc agtaacgttg gcccgatggt ccgcgccagc
    32941 ggacacgctc gcgatacccg cgccgatcac ccgtttgttg gttatggcct gctgccaatg
    33001 gaagtccaca gcgagcaggg ctgcgacgtt atttcccgtc tgaaagtgcg tatcaacgaa
    33061 gtctataccg cgctgaacat gatcgactac ggtctggata acctgccggg tggtccgctg
    33121 atggtggaag gctttaccta cattccgcac cgtttcgcgc tgggctttgc cgaagcgccg
    33181 cgcggcgacg atatccactg gagcatgacc ggcgacaacc agaagctgta ccgctggcgc
    33241 tgccgtgccg cgacctacgc gaactggccg accctgcgct acatgctgcg tggcaatacc
    33301 gtttccgatg cgccgctgat tatcggtagc ctggaccctt gctactcctg taccgaccgc
    33361 atgaccgtgg tcgatgtgcg taagaagaag agcaaagtgg tgccgtacaa agaactcgag
    33421 cgttacagca ttgagcgtaa aaactcgccg ctgaaataag gaatcgccat gtttaccttt
    33481 atcaaaaaag tcatcaaaac cggcacggcg acctcgtctt atccgctgga gccgattgcg
    33541 gttgataaaa acttccgtgg taagccagag cagaacccgc agcagtgcat cggctgcgcg
    33601 gcctgcgtca atgcctgccc gtcaaacgcc ttaacggttg aaactgacct cgccacagga
    33661 gagcttgcgt gggagtttaa tctcgggcgc tgcatcttct gtggacgctg cgaagaagtc
    33721 tgcccgacgg cagcgatcaa actgtcgcaa gagtacgaac tggcggtgtg gaagaaagaa
    33781 gatttcctgc aacagtcccg ttttgtgctg tgcagctgtc gcgtctgcaa tcgtcctttc
    33841 gccgtccaga aagagatcga ctacgccatt gcgctgctta agcacaatgg tgacagccgc
    33901 gcggaaaacc accgcgaaag ctttgagact tgcccggaat gtaagcgcca gaaatgcctg
    33961 gtgccgtccg accgtattga actgactcgc catatgaaag aggccatctg atgagcaatt
    34021 tattaggccc ccgtgacgcc aacggcattc cggtcccgat gacggtggat gaatccatcg
    34081 ccagcatgaa ggcgtcgtta ctgaaaaaaa tcaaacgttc tgcctacgtt taccgcgtgg
    34141 actgcggcgg ctgcaacggc tgcgaaatcg aaattttcgc cacgctttcg ccgctgtttg
    34201 atgcagaacg cttcggcatt aaagtcgttc cttcaccgcg tcatgcggat attttactgt
    34261 ttaccggcgc ggtcacccgt gcaatgcgat cccctgcgct gcgtgcgtgg cagtccgcgc
    34321 cggacccgaa aatctgtatc tcctacggtg cctgcggtaa cagtggcggg atcttccacg
    34381 atctctactg cgtgtggggc ggtacggata aaatcgtccc ggtagatgtc tacatccctg
    34441 gttgcccgcc aacgcctgcc gccacgctgt acggctttgc aatggcgctc ggcctgctgg
    34501 agcagaaaat tcacgcccgt ggcccgggtg agcaggatga acaaccggcg gaaatcctgc
    34561 atggcgatat ggtgcagccg ctgcgtgtga aagtggatcg cgaagcacgc cgcctggcag
    34621 gttatcgtta cggtcgtcag attgccgatg acttcctgac acagttaggg cagggcgaag
    34681 aacaggttgc acgctggctg gaagcggaaa acgatccgcg tctgaacgag attgtcagcc
    34741 atctgaatca tgttgtagaa gaggcgcgta tccgatgagt gaaaaggtgg tgttcagtca
    34801 actgagccgt aaatttattg atgagaacga tgccacgccc gccgaggcgc agcaggtggt
    34861 ctattacagc ctggcgattg gtcaccacct tggggttatc gattgcctgg aagcggcgct
    34921 cacctgcccg tgggatgaat atctggcatg gattgccact ctggaggcgg gcagcgaagc
    34981 ccgccgcaaa atggaaggcg tgccgaaata tggtgagatc gtcatcgaca ttaaccatgt
    35041 gccgatgctg gccaacgcat tcgataaagc ccgggcagcg caaacttcgc agcagcagga
    35101 atggagtaca atgctgctaa gtatgctgca tgatattcat caggaaaacg ccatctattt
    35161 gatggtgagg agactgcgtg actgacgttt tactctgtgt tggcaatagc atgatgggcg
    35221 atgatggcgc aggcccgctg ctggcggaaa agtgcgccgc cgcgccgaaa ggtaactggg
    35281 tggtgattga cggcggtagt gcaccggaaa acgacatcgt cgccatccgt gaactgcgcc
    35341 ctacgcgact gctgattgtc gacgccactg atatggggct aaaccccggc gagatccgca
    35401 tcatcgaccc ggatgatatc gccgagatgt ttatgatgac tacccataac atgccgttga
    35461 actaccttat cgaccagttg aaagaagata ttggcgaagt gattttcctc ggcatccagc
    35521 cggatatcgt cggcttttac tacccgatga cccagccgat taaagatgcg gttgaaaccg
    35581 tttatcaacg actggaaggc tgggaaggga atggcggctt cgcgcagtta gcggtggaag
    35641 aagaataatt ctccatcagg gaattcagcg aaatgaatat cagaacaggg atgtgcgctt
    35701 ttttattgag cttagtgctg cctgcacagg ccaccagctt taccgaatat ctgccaatga
    35761 gtgacagcga atacgcgcaa aagcgggcgc tgaaacctct cctgaccatg ccttatgatg
    35821 ccgagcaaac ctggcatttt agaaaagtgg gcgttgctgg cgtgacgctt gaaaagatgc
    35881 caaatgatga tagtgaatgg caattgaacg gtaaagatcg cgccggtaaa tcctggtctg
    35941 tgcctgtggg tatgctggca aacatagcgg gcaaagggca actctatcgc gccgatcttg
    36001 atcgtaacgg cattcaggat ctggttatct ggttaccaag taccggtaat ggtctggctc
    36061 cttatgcaca tttaattctt atgacgttca cgcgcgaagg ccgtccctgt gtctttgaac
    36121 ccaggggatt ctacaccgca agcaaaactg gcgtagatga tttgctggat ttgcaaggca
    36181 atggtcacac gcaattgctg gatatgcagt ttaattctgg ttactggata accagtctgt
    36241 atcaggtaaa agacgccaaa tggcagcgtg ttcacggctg gtttggcaag ttaagctatc
    36301 ccgcgctcac gcgttttact tataccccca accgtaaact cgtcctcaaa cccatcgccg
    36361 ggcgcgaccc gcaaacggaa gatctggcgc agacgcagcg ttgcttgata aagggggatg
    36421 tgcttgaggg gtgaaccaga actgactttt tgcagagtgg ctatgaggat tttgggggta
    36481 attatatcgc gcacaagtgt ttacatattg tgaacttgga tttgatgagc tatagtttcc
    36541 gaacacataa ggatagaaca tcgcagatgt ggctgtttac ttactatcag gaagttaaaa
    36601 cttaaaaatg cacatcattt ctaaagcgcc atttgaagag tctgcgcgta aatatcctaa
    36661 cgacgctctg gctcttcagg cattgtatcg ggttattaag gaaacggatt tttctacacc
    36721 agaagaaatg cgaacggcgt ttcccaacct cgacaatttt agatatcgca acaaatggta
    36781 cgttctggat gtcggcggga acaatcttag ggttattgcc tacataaact ttgttaataa
    36841 gcggttcttt gtgaagcaca taacgaatca cgcagagtat gacaaactga cacgttacta
    36901 cagagagaac aaagaatgac tgctaatgct gcacgcgctg ttaaagcaac aagagaactg
    36961 gtcaatgcag tcccctttct gggaggcagt gactcagaag atgattatcg tgaagcactg
    37021 gaactggtcg aatatttgat tgaagaagat gacaccaatc cacttatcga ctttctggcg
    37081 agccgcattg cagagtatga aaacaacaac gaaaagtttg cagagttcga caaagccgtt
    37141 gcggcaatgc cggttggcgt ggcgttactt cgcacgttga ttgatcaaca taatctgacc
    37201 tatgcggatt tgaagaacga aatcggctct aaatccctgg tcagtcagat tttgtctgga
    37261 caacgatccc tgacgatatc ccacattaag gcactttcag cccgatttgg cgttaagccg
    37321 gaatggtttc tttgattttg actgatgcca aaccccagat tgcccgatgc gctgcgctta
    37381 tcgggccttc atggttcgtg cgacatgtag gccggataag gcgttcacgc cgcatccggc
    37441 actgttacct actctaaatc ttccccatta ctggcaatca cttttttata ccaccagaat
    37501 gatttcttac gcgtgcgcgt cagcgtaccg ttgcctgcgt cgtcacggtc aacaaagaca
    37561 aagccgtagc gtttgctcat ttcacccgta gaggcggaaa ctaaatcaat acagccccat
    37621 gtggtgtagc ccatcagcgg aatgccgtct gcaatcgctt cgcccattgc gcggatatgt
    37681 tcgcgtaagt agctgatgcg atagtcgtcg ttaatctcgc cattggcagc aaattcatct
    37741 tttgcgccca ggccgttttc caccagaaac agcggcttct gataacgatc gtacatcata
    37801 ttcatggtga tacgcagacc gagtggatca attccccagc cccagtcgct cacctgtaga
    37861 tacggattac gcagcgattt caccacgttc gccgcactgc tgttgttggc gttcatctcc
    37921 gccgaggcgc agcgcgaggc gtaatagctg aaagagacaa aatcgacggt gtttttcagg
    37981 atttcatcat cgcccggtgc tttgttgatg gttacccctt tttcgcggaa tacgcgggca
    38041 gagtaagccg gatacgtgcc ccgcgcctgc acatcgataa aaaacaggtt ttcccggtct
    38101 ttctccagcg ccgcccagac atcttccggc ttgcaactgt aagggtagaa gttaccgccc
    38161 gccagcatac agcccacctg attttgcggg ttaacctcat gggcgatttt ggtggctagc
    38221 gcactggcaa ccagctggtg atgcgcggcc tgatatttca cctgatcctg attttcacct
    38281 tcttcaaaca ccagacccgc gccggagaac gggctatgca acataatgtt gatttcattg
    38341 aaggttagcc agtatttcac cagaccatca aatgcttcaa agcaggttct ggcgtagcgg
    38401 ctgaaaaact ccaccagctt gcggttacgc caggagccat attcggtgac cagatgcatc
    38461 ggcacatcga agtggcacaa cgtgaccagc ggttcgatac cgtacttttt acactcttca
    38521 aagacagaac gataaaaagc aatgccctgc tgattgggcg tgatttcatc gccctgcgga
    38581 aagagacggc tccaggcaat tgaggtacgg aaaaccttga atcccatctc tgccatcagg
    38641 gcgatatctt ctttataacg atgataaaaa tccgtcgcct catggctggg ataaaactcg
    38701 tcatctcgca actgaaaacg tttttccagc cccagtttaa ccgccattcg atgctcgccg
    38761 tgtgggatca tatcgacagt ggtcagacct ttgtcacctt cacggaacgc accttcagac
    38821 tggttggcgg caagcgcgcc gccccataaa aaactttctg gaaatactga cattttcatc
    38881 ctcaattaag acttacttct ttggcgacgg tcggttgtac gctctgatac tttcgcgcct
    38941 gggcagccgc ttcctcaaca ggaatatcct caaagccgag caacagtgtg aggataaacg
    39001 acagcaccac cgccagcgcc atgaccgcga acacccagac gatgctcatt ggattcgccg
    39061 gatcgaagaa ctgcacgctg gtaaatagcc ccggcgctgc cattgagtgg ctggcaagcc
    39121 ccgccatacc ggcaaccgcg ccgcaaataa aaccgctgat aagactggcg ataagcggac
    39181 gtttcaggcg gatcgccacg ccatataacg ccggttcgga aatccccgcc ataatggctg
    39241 atgccgccgc agccagcgcc gtctgacgca gttccgggtt tttcgttttc cacgccactg
    39301 ccagtgatga accgcccagc gacaggttag cgccgatctc tgacggcatg accatccctt
    39361 ctttgccggt ttcggcaatg gtctgaatga tggttggcgt aaagacgcgg tgcatcccgg
    39421 tcattaccag cagaggccac agcgcgccca taatggcgac tgaaagccag cccagataac
    39481 catgaatggt gtaaaccagc gccgaaatgg cgctaccgat ccagatacca atcgggccaa
    39541 tcagcaggat tgccagcggt gcggcaatca acacaatcag cattggtttg aggaagtttt
    39601 tggtcaccgc cggtgtaatg cgatccaccc agcgttcgat atatgacagg caccaggtca
    39661 tcaccagcgc cgggatcacc gtgtaggtat atttcaccgc ggtgaccgga atcagggcaa
    39721 attcgacatg ttcaccctgg gccgctttcg ccatcagttc aataaaactc ggatgtacca
    39781 gcacacccgc gatggcaatc gccagcgaca tattggtttt aaatttgatg gcggcagatg
    39841 ccgcgaccat cagcggcagg aagaagaaag caccgtcacc aatcacattc agaatggtta
    39901 aggtcggcga tccttttgtc agcacgccgc tcatctcgag gatcatcgcc agcagtttga
    39961 ccatcgatcc gccgataatc gccgggatca gcggtgacat ggtgccgatc agcgcatcga
    40021 ggatccctgc accaatgcga cgcagcgtga gtttgggttt acccacgggc tgtgcgggct
    40081 gcatatctcc cggcagcagg ctgacgactt cctgaaaggc ttgtgaaacg gtattgccga
    40141 taatcacctg acactggttg tcactacgta ccacgccgag cacgccgggg atggttttta
    40201 acgtcgggct gtcgataagc tggtcatctt tgataacaaa gcgcaaacgc gtcatacagt
    40261 gcgtgaccgc cgagatgtta tcaacgccgc ccagtgccgc tatcaccgag cgtgccagcg
    40321 ccgcataatt tttggccatc ggattttatc ctgttatcag tagggtagat gtagttgtta
    40381 tcagtcgacg taacggaaaa atgccgatgc tgaaacggaa ttaggaaacc ggtttcacaa
    40441 aatcatgaat agttgtcact aaataagcaa gagagttaat tgttcatttt ttatatttgt
    40501 gaaaccggtc acctgaatat ttttcccgcc gattgcgcaa ggacaatcgg caaacggtgc
    40561 agtacactgc gttgattgcg tgtaaaggga tgaaaatgat gacgacgatg ctggaagtgg
    40621 cgaagcgcgc cggggtttca aaagcgaccg tttcccgcgt gctttcaggt aatggctacg
    40681 tcagccagga gactaaagat cgcgtgtttc aggcggtaga agagagcggt taccgcccaa
    40741 atttgctggc ccgcaatctg tcggcgaaga gtactcagac gctggggctg gtagtgacca
    40801 acacgcttta tcacggcatt tattttagtg aattactgtt tcatgccgcg cgaatggcgg
    40861 aagagaaagg gcgacagttg ctattggcag atggtaaaca cagcgccgaa gaagagcgcc
    40921 aggcgattca gtatctgctg gatttgcgct gcgacgccat tatgatttac ccgcgttttt
    40981 taagcgtgga tgagattgat gacatcattg acgcgcacag tcagccgata atggtgctta
    41041 atcgccgcct gcgcaaaaat agcagccata gcgtctggtg cgatcataaa cagaccagct
    41101 ttaacgccgt ggcagagttg ataaacgccg ggcatcagga gattgctttc cttaccggct
    41161 cgatggattc ccccaccagc attgaacgtc ttgccgggta taaagacgcg ctggcgcagc
    41221 atggtattgc gctcaatgaa aaacttatcg ctaacggtaa atggacgcct gccagcgggg
    41281 ccgaaggggt agaaatgttg ctcgaacgtg gggctaaatt tagcgcgtta gttgccagta
    41341 acgacgatat ggcgataggt gcgatgaaag cgttacacga gcgcggcgta gcggtgccag
    41401 agcaggtgtc agttatcgga ttcgatgata tcgctattgc cccctacacc gttccggcgc
    41461 tctccagcgt aaaaattccg gtgactgaga tgattcagga aattattgga cggctgattt
    41521 ttatgctcga tggcggggat ttctcaccgc cgaaaacctt cagcggaaaa ctg
//
LOCUS       DAFNBJ010000020        41370 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000020 DAFNBJ010000000
VERSION     DAFNBJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.020"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            99..203
                     /locus_tag="L2X49_001095"
     CDS             99..203
                     /locus_tag="L2X49_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBN4796217.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            514..2712
                     /gene="speF"
                     /locus_tag="L2X49_001096"
     CDS             514..2712
                     /gene="speF"
                     /locus_tag="L2X49_001096"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HBN4796218.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKETRPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2709..4028
                     /gene="potE"
                     /locus_tag="L2X49_001097"
     CDS             2709..4028
                     /gene="potE"
                     /locus_tag="L2X49_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBN4796219.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4084..5724)
                     /gene="pgm"
                     /locus_tag="L2X49_001098"
     CDS             complement(4084..5724)
                     /gene="pgm"
                     /locus_tag="L2X49_001098"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBN4796220.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHTLSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     IDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5750..6295)
                     /gene="seqA"
                     /locus_tag="L2X49_001099"
     CDS             complement(5750..6295)
                     /gene="seqA"
                     /locus_tag="L2X49_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBN4796221.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6480..7244
                     /gene="ybfF"
                     /locus_tag="L2X49_001100"
     CDS             6480..7244
                     /gene="ybfF"
                     /locus_tag="L2X49_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBN4796222.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7384..7677
                     /gene="ybfE"
                     /locus_tag="L2X49_001101"
     CDS             7384..7677
                     /gene="ybfE"
                     /locus_tag="L2X49_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBN4796223.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7817..8347
                     /gene="fldA"
                     /locus_tag="L2X49_001102"
     CDS             7817..8347
                     /gene="fldA"
                     /locus_tag="L2X49_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBN4796224.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8557..8643
                     /gene="uof"
                     /locus_tag="L2X49_001103"
     CDS             8557..8643
                     /gene="uof"
                     /locus_tag="L2X49_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBN4796225.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8636..9082
                     /gene="fur"
                     /locus_tag="L2X49_001104"
     CDS             8636..9082
                     /gene="fur"
                     /locus_tag="L2X49_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBN4796226.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9167..9493)
                     /locus_tag="L2X49_001105"
     CDS             complement(9167..9493)
                     /locus_tag="L2X49_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796227.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9543..10949)
                     /gene="chiP"
                     /locus_tag="L2X49_001106"
     CDS             complement(9543..10949)
                     /gene="chiP"
                     /locus_tag="L2X49_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBN4796228.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERNRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11529..13193)
                     /gene="glnS"
                     /locus_tag="L2X49_001107"
     CDS             complement(11529..13193)
                     /gene="glnS"
                     /locus_tag="L2X49_001107"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBN4796229.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13461..15407)
                     /gene="nagE"
                     /locus_tag="L2X49_001108"
     CDS             complement(13461..15407)
                     /gene="nagE"
                     /locus_tag="L2X49_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBN4796230.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVRAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15740..16540
                     /gene="nagB"
                     /locus_tag="L2X49_001109"
     CDS             15740..16540
                     /gene="nagB"
                     /locus_tag="L2X49_001109"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBN4796231.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16600..17748
                     /gene="nagA"
                     /locus_tag="L2X49_001110"
     CDS             16600..17748
                     /gene="nagA"
                     /locus_tag="L2X49_001110"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBN4796232.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17757..18977
                     /gene="nagC"
                     /locus_tag="L2X49_001111"
                     /gene_synonym="nagR"
     CDS             17757..18977
                     /gene="nagC"
                     /locus_tag="L2X49_001111"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBN4796233.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            19025..19777
                     /gene="nagD"
                     /locus_tag="L2X49_001112"
     CDS             19025..19777
                     /gene="nagD"
                     /locus_tag="L2X49_001112"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBN4796234.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            20174..21838
                     /gene="asnB"
                     /locus_tag="L2X49_001113"
     CDS             20174..21838
                     /gene="asnB"
                     /locus_tag="L2X49_001113"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBN4796235.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVTDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            22217..22293
                     /locus_tag="L2X49_001114"
     tRNA            22217..22293
                     /locus_tag="L2X49_001114"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22251..22253,aa:Met,seq:cat)
     gene            22302..22386
                     /locus_tag="L2X49_001115"
     tRNA            22302..22386
                     /locus_tag="L2X49_001115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22336..22338,aa:Leu,seq:tag)
     gene            22410..22484
                     /locus_tag="L2X49_001116"
     tRNA            22410..22484
                     /locus_tag="L2X49_001116"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22442..22444,aa:Gln,seq:ttg)
     gene            22519..22593
                     /locus_tag="L2X49_001117"
     tRNA            22519..22593
                     /locus_tag="L2X49_001117"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22551..22553,aa:Gln,seq:ttg)
     gene            22609..22685
                     /locus_tag="L2X49_001118"
     tRNA            22609..22685
                     /locus_tag="L2X49_001118"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22643..22645,aa:Met,seq:cat)
     gene            22734..22808
                     /locus_tag="L2X49_001119"
     tRNA            22734..22808
                     /locus_tag="L2X49_001119"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22766..22768,aa:Gln,seq:ctg)
     gene            22846..22920
                     /locus_tag="L2X49_001120"
     tRNA            22846..22920
                     /locus_tag="L2X49_001120"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22878..22880,aa:Gln,seq:ctg)
     gene            complement(23074..24249)
                     /gene="ubiF"
                     /locus_tag="L2X49_001121"
     CDS             complement(23074..24249)
                     /gene="ubiF"
                     /locus_tag="L2X49_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBN4796236.1"
                     /translation="MTNQPTEIAIVGVGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            24395..25819
                     /gene="miaB"
                     /locus_tag="L2X49_001122"
     CDS             24395..25819
                     /gene="miaB"
                     /locus_tag="L2X49_001122"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBN4796237.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25972..27012
                     /locus_tag="L2X49_001123"
     CDS             25972..27012
                     /locus_tag="L2X49_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBN4796238.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            27009..27476
                     /gene="ybeY"
                     /locus_tag="L2X49_001124"
     CDS             27009..27476
                     /gene="ybeY"
                     /locus_tag="L2X49_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBN4796239.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQKESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            27566..28444
                     /gene="corC"
                     /locus_tag="L2X49_001125"
     CDS             27566..28444
                     /gene="corC"
                     /locus_tag="L2X49_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBN4796240.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            28469..30007
                     /gene="lnt"
                     /locus_tag="L2X49_001126"
     CDS             28469..30007
                     /gene="lnt"
                     /locus_tag="L2X49_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBN4796241.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(30085..31962)
                     /locus_tag="L2X49_001127"
     CDS             complement(30085..31962)
                     /locus_tag="L2X49_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBN4796242.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            32417..33325
                     /gene="gltI"
                     /locus_tag="L2X49_001128"
     CDS             32417..33325
                     /gene="gltI"
                     /locus_tag="L2X49_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBN4796243.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            33495..34235
                     /gene="gltJ"
                     /locus_tag="L2X49_001129"
     CDS             33495..34235
                     /gene="gltJ"
                     /locus_tag="L2X49_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBN4796244.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            34235..34909
                     /gene="gltK"
                     /locus_tag="L2X49_001130"
     CDS             34235..34909
                     /gene="gltK"
                     /locus_tag="L2X49_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBN4796245.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            34909..35634
                     /gene="gltL"
                     /locus_tag="L2X49_001131"
     CDS             34909..35634
                     /gene="gltL"
                     /locus_tag="L2X49_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBN4796246.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKCPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            35752..36687
                     /gene="rihA"
                     /locus_tag="L2X49_001132"
     CDS             35752..36687
                     /gene="rihA"
                     /locus_tag="L2X49_001132"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBN4796247.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            36771..38441
                     /gene="hscC"
                     /locus_tag="L2X49_001133"
                     /pseudo
     CDS             36771..38441
                     /gene="hscC"
                     /locus_tag="L2X49_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(38501..39952)
                     /gene="djlC"
                     /locus_tag="L2X49_001134"
     CDS             complement(38501..39952)
                     /gene="djlC"
                     /locus_tag="L2X49_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBN4796248.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(39949..40656)
                     /locus_tag="L2X49_001135"
     CDS             complement(39949..40656)
                     /locus_tag="L2X49_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBN4796249.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            40758..41312
                     /locus_tag="L2X49_001136"
     CDS             40758..41312
                     /locus_tag="L2X49_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBN4796250.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
BASE COUNT        10224 a        10535 c        10342 g        10269 t
ORIGIN      
        1 gggtatgatc gttttttgtt gatcttcttc acagattata gccatttcat ggatagaata
       61 actctacctt caactgacac agcaagaggt aaaggtaaat ggaaaataat aaccgcttaa
      121 tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat agttttgact
      181 ttcatttctt taatgcccgc tagtcttctg actaaagggc accccaacgt acaggtctcc
      241 ctgactttaa gcattacagg ttaatacctg tattcctcgg tgctcatata ctgctaaccc
      301 ttttaaactc taaataattc gagtcgcagc acttgcaact tgaggtatga cgagtatagc
      361 cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg atttatttat
      421 cagcggtgga gctttgcttt ttttccggcg tgatcgattt ctcctttgag aaattgagga
      481 cctgctatta cctgaaataa agagatgaac aaaatgtcag aattaaaaat tgccgttagt
      541 cgttcttgcc cggattgttt ttccactcat cgtgcatgcg tgaatataga cgaaagtaat
      601 tatattgacg ttgccgccat tattttatca gtcagtgatg ttgaacgtgg aaaactcgat
      661 gaaatagacg ctactggcta tgacattcct gtttttattg caacggaaaa tgaagaacgt
      721 gtacccgcag aatatctgcc acgtatttct ggtgtcttcg aacattgcga atcacgtaaa
      781 gagttttatg gacgtcagtt agaaaccgct gccagccatt atgaaacgca actgcgcccg
      841 cccttcttcc gcgcactggt cgattatgtt aatcagggca atagtgcttt tgactgccct
      901 ggtcatcagg ggggtgagtt tttccgtcgc catccggcag gcaatcagtt cgtggaatac
      961 tttggcgaaa tgctcttccg ctctgacttg tgtaatgctg acgtagcaat gggcgatctg
     1021 ctgattcatg aaggtgcgcc atgtattgca caacagcatg cggctaaagt ctttaatgcc
     1081 gataaaacct atttcgtttt aaacggtact tcatcttcta ataaagtggt cttaaacgca
     1141 ttgctgacgc cgggcgatct ggtgttgttt gaccgcaata accataaatc taaccaccac
     1201 ggtgcgttgc tacaggctgg cgcgacaccg gtttatctgg aaaccgcacg taacccgtat
     1261 ggctttatcg gtggcattga tgcgcactgt tttgaagaaa gttacctgcg tgagctgatt
     1321 acagaagttg caccgcagcg ggcaaaagag actcgtcctt tccgcctcgc cgttattcag
     1381 ctaggcactt acgacggtac gatttataac gcccgtcagg tggtggataa aattggtcat
     1441 ctgtgtgact acatcctctt tgactccgca tgggtcggct atgaacagtt tattccgatg
     1501 atggctgact gttcgccgct gttgctggat cttaatgaga acgatccggg tattctggtt
     1561 acgcaatccg tacataaaca gcaggctggt ttttctcaaa cttcacaaat ccataaaaaa
     1621 gacagccaca tcaaagggca acagcgttat gtaccgcaca aacgcatgaa caacgccttt
     1681 atgatgcacg cctccaccag cccgttctat ccgctgtttg ccgcactgga tatcaacgcc
     1741 aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt aaatggcatt
     1801 aatgcccgca aactgatcct cgataactgt cagcatattc gtccgttcgt acctgaactg
     1861 gtggatggta aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga tctgcgcttc
     1921 ttccagtttg taccagggga acactggcat tcttttgaag gctatgcaga gaatcaatac
     1981 tttgtcgatc cttgcaaact gttgctgaca accccaggta ttgatgcacg taacggcgaa
     2041 tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga aaatggcgta
     2101 gtgccggaaa aatgcgatct taactccatc ctcttcctgc tgactccggc agaagatatg
     2161 gccaagcttc agcaacttgt tgccctgctg gtacgcttcg aaaaactgct tgagtccgac
     2221 gcgccattag cagaagtgct gccttccatc tacaaacagc atgaagagcg ctacgccggt
     2281 tataccctgc gtcagttgtg tcaagaaatg catgatttgt atgcccgtca caacgtgaaa
     2341 caactgcaaa aagagatgtt ccgtaaggaa cacttcccac gcgtaagcat gaatccgcaa
     2401 gaagccaact acgcctattt acgcggtgaa gtggagctgg ttcgtctgcc ggatgcagaa
     2461 ggccgtatcg ctgccgaagg tgcgcttcct tatcctccgg gtgtgctgtg cgttgttccg
     2521 ggtgaaatct ggggtggtgc tgttctgcgt tacttcagcg ctctggaaga agggatcaac
     2581 ctgttgccag gttttgcacc ggagcttcag ggtgtctata tcgaagaaca tgatggtcgt
     2641 aagcaagttt ggtgctatgt catcaagcct cgtgatgcgc aaagcaccct gttgaaaggg
     2701 gaaaaattat gagtcaggct aaatcgaaca aaatgggcgt cgttcagtta accatactga
     2761 cgatggtcaa catgatgggc tccggtatca tcatgctgcc gacaaagctt gccgaagttg
     2821 ggacaatttc aattatctcc tggctggtga cagccgtagg ctcaatggca ctggcatggg
     2881 cattcgcaaa atgcggtatg ttcagccgta aatcaggtgg tatgggcggt tatgccgagt
     2941 atgctttcgg taagtccggc aattttatgg cgaactacac ctacggtgtc tcgctgctga
     3001 ttgctaacgt cgcgattgct atttcggcgg ttggttacgg caccgaactg ctcggcgcga
     3061 gtttgtcgcc agtgcagatt ggtcttgcga ccatcggagt gctgtggatt tgtaccgtgg
     3121 ctaactttgg tggtgcgcgc attaccgggc aaatcagtag cattactgtg tggggggtca
     3181 ttattccggt ggtaggcctg tgcattatcg gctggttctg gtttagcccg acgctttacg
     3241 ttgattcctg gaatccgcat catgcaccgt tcttcagtgc agtaggttct tccatcgcta
     3301 tgacgctgtg ggcttttctc ggtctggagt ctgcgtgtgc gaatactgat gtagtggaaa
     3361 acccggaacg taatgtgcca atcgcggtac tcggcggtac gttaggtgcg gcggtgattt
     3421 atatcgtctc caccaacgtg attgcaggga ttgtgcctaa tatggagctg gcaaattcaa
     3481 cggcaccatt tggcctggcc ttcgcacaga tgttcacacc agaagtgggt aaagtcatta
     3541 tggcgctgat ggtgatgtcc tgctgcggtt cgctgcttgg ctggcagttc accattgccc
     3601 aggtgtttaa atcttcatct gatgaaggct acttccctaa aattttctcc cgtgtaacca
     3661 aagtggatgc accggtgcag ggaatgttga ccattgtgat tatccagagc gggttggcgc
     3721 tgatgacgat tagcccgtcg ctaaacagtc agttcaacgt gctggttaac ctggccgtgg
     3781 tgaccaatat cattccgtat attctgtcaa tggcggcatt agtcattatt cagaaggtcg
     3841 ctaatgtgcc cccctcaaaa gcgaaagttg caaactttgt tgcttttgtt ggcgcgatgt
     3901 atagctttta tgcgctgtac tcatccgggg aagaagccat gctgtacggt tccatcgtaa
     3961 ccttccttgg ttggacactg tatggtctgg tatcaccacg ctttgaactg aaaaataaac
     4021 acggttaaaa aagcaaagta aaaaagggcg atcttgcgac cgcccttttt ttattaaatg
     4081 tgtttacgcg tttttcagaa cttcgctaac aatctcaacc gcttctttct caatctgctt
     4141 gcgatgttct tcaccgagga agctttcgca gtagatctta tatgcgtctt ccgtgcctga
     4201 cggacgcgcg gcgaaccagc cgttgtcagt catcactttc agaccgccga tagaagcacc
     4261 attgcccggt gccgccgtca ggcgcgcggt gatcgggtcg cctgccaggg tgctggcgct
     4321 caccatttcc ggagacagct tagacagcgc cgctttttgt gcggaagtcg cagctgcctg
     4381 caaacggttg tagctcggcg caccaaagcg ttttgccagt tcgttgtagt gttcctgcgg
     4441 gttcttaccg gtgaccgcgg tgatttccgc cgccagcaga cacatgatga tgccgtcttt
     4501 gtcggtggac cacggcgtgc cgtcgaaacg caggaaggaa gcccctgcgc tctcttcgcc
     4561 gccaaaaccg aagctgccgt cgaacagacc atcaacaaac catttgaaac ctaccggtac
     4621 ttctaccagc ttacggccca agtcattgac cacacggtcg atcatcgcag aagaaaccag
     4681 cgttttaccg acagcaacat ctttgcccca ctgcggacga tgctggaaca ggtagttgat
     4741 cgccaccgcc aggtagtggt tcggattcat caaacctgcc ggagtgacga taccgtgacg
     4801 gtcataatcc gggtcgttag caaacgccag atcgaactta tcacgcagtg ccagcaggcc
     4861 cgccatcgca cactcggagg agcagtccat acggatcgcg ccgtctttat caaggtgcat
     4921 aaagcggaag gtttgatcga cctgatcgtt aacgatggtc aggttgaggt tgtaatactc
     4981 gccaatacgc ttccagtatt cgataccgga accgcccagc ggatcaacgc ccagcgtcag
     5041 gcccgctttc tgaattgccg ccatatcaat gatatcggcc agaccttcca caaacggctg
     5101 caccagatcc tgctctttta catgaccgga tgccatcgct tcgtcgaggg agatacgctt
     5161 cacgcctttc aggccatcgg ccatcagtgc gttggccctg tcttccacca ctttggtgac
     5221 gttggtatca gccgggccac catttggcgg gttgtacttg ataccaccat cttccggcgg
     5281 gttatgggac ggtgtaatca cgataccgtc tgccagcggg ccaccttttt tattgtgaac
     5341 caggatggca ttggaaatgg caggtgtcgg agtgaagcca ttgttttcct gcacaatgac
     5401 atcaacgccg ttcgctgcca gcacttccag tacggaaatg aacgcaggct cggacagggt
     5461 gtgagtatct ttacccacat agcaagggcc agtgatgccg tttttcgcac gttcttcagc
     5521 aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag ctgtggcgcg ctgcactgcc
     5581 acggtgaccg gaagtaccga atttcaccgc gtgctccgca ttccctgctt ctggtttcag
     5641 tacataatat tgcgccgtca gttgggcgac gttaatcaaa tcactctgtt gtgcaggttg
     5701 gcctgcacga ttgtggattg ccattgcttt gtcctttgtc tgcaacgttt tagatagttc
     5761 cgcaaacctt ctcaatcaat tccgccggga attgcatcga ctgcatgatg tgttcgatca
     5821 tgctgcattt acggccggtg ttggtgttgg tgatcaccca atacggcgtg cctggcacat
     5881 gtttcggctt ggtctgatta ccatttttca gcagcgtttg ttcatctgcc gcaaagtaaa
     5941 cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa cgcctgggcg tcaagagaat
     6001 atagtgtaga caacagcagc ataaagcgat tgaccgctcg cttttgctct gcgtattcgt
     6061 ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt aatcgttttg accggcttcg
     6121 cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt caccggagca gcaggctgtg
     6181 atgcggcgga aaatttcaac atacgccgta aaatgtcgga tgcgctctcg ccgatatgct
     6241 tagtgtggct ggcaatatag ctgtagagtt catcatcaac ttcaatcgtt ttcatcttaa
     6301 tccagtgcag tgtcttagct gaatacaagt cgccaggagt atatgaataa atcccggcag
     6361 cggatagcgt caagcctggc ttgcagaaaa actcggaata aaccttattt gttgcagccc
     6421 tgtggcacaa tggtcaacat aataccctaa cccgacaacg cgaaaaaaag aactttgcca
     6481 tgaaattgaa tatccgcgcg caaactgcac aaaaccagca caataattct cccatcgttc
     6541 ttgtccatgg tctgtttggt agcctcgaca accttggcgt actggctcgc gatctggtaa
     6601 acgatcacaa tatcatccag gttgatatgc gtaaccacgg tctttcaccg agagagccgg
     6661 taatgaatta cccggcgatg gcgcaggatc ttgttgatac cctggatgca ctgcagatcg
     6721 acaaagcaac atttatcggt cactccatgg gcggtaaagc ggtaatggca cttactgcac
     6781 tggcccccga tcgcatcgat aaactggtgg cgatcgatat cgcaccggtc gactatcacg
     6841 tacgccgtca tgatgagatt ttcgcagcta tcaacgcggt cagtgaatcg gacgcacaaa
     6901 ctcgccagca agcagcagca ataatgcgcc agcatcttaa tgaagaaggg gtgattcagt
     6961 ttctgttgaa atcttttgtt gacggggagt ggcgctttaa cgtgccggta ttgtgggatc
     7021 agtatccgca tattgtgggt tgggagaaaa tcccggcatg ggatcaccct gccctgttta
     7081 tccctggcgg caattctccg tatgttagcg agcagtaccg tgatgattta ctggcacaat
     7141 ttccacaggc acgggcgcat gtgattgcag gcgcgggtca ctgggtccat gctgaaaaac
     7201 cggatgcggt attacgcgct atccgtcgct atctcaatga ttaacgcaac tgattaaaaa
     7261 cccagcgtcc gcgtcgcttt ccgcgcgtgg gcgttggcgt gactcatcgc gctgatgtat
     7321 tatggcgcgc tatccatccg ggcagaggcc tggctgattg tttcccccga agtcaccaag
     7381 atcatggcca aagaacaaac ggaccgtacg acattagatc tgttcgcgca cgagcgtcga
     7441 ccgggacgac cgaaaactaa tccgctttcg cgcgatgaac agctgcgtat taataaacgc
     7501 aaccagctaa aacgcgacaa agtacgtggc cttaagcgtg tcgaactgaa gctgaacgcg
     7561 gaagctgtcg aggcgctgaa cgagctggcg gagtcgcgca atatgagccg tagcgaactg
     7621 atcgaagaga tgttaatgca gcaactggcg gcgctgcgta gtcagggcat cgtttaaatt
     7681 tccactttca tgtagcacag tgtgcagtcc tgctcgtttg ctgattccgc aagactgcct
     7741 gttctgctat gattgccttt atccgtgggc aattttccac ccccatttca ataagtttca
     7801 agaggttatt tcactcatgg ctatcactgg catctttttc ggcagcgaca ccggtaatac
     7861 cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa gacgttgccg atgtccatga
     7921 cattgcaaaa agcagcaaag aagatctgga agcttatgac attctgctgc tgggcatccc
     7981 aacctggtat tatggcgaag cgcagtgtga ctgggatgac ttcttcccga ctctcgaaga
     8041 gattgatttc aatggcaaac tggttgcgct gtttggttgt ggtgaccagg aagattacgc
     8101 cgaatatttc tgcgatgcat tgggcaccat ccgcgacatc attgaaccgc gcggtgcaac
     8161 gatcgttggt cactggccaa ctgcgggcta tcatttcgaa gcatcaaaag gtctggctga
     8221 tgacgaccac tttgtcggtc tggctatcga cgaagaccgt cagccggaac tgaccgctga
     8281 acgtgtagaa aaatgggtta aacagatttc tgaagagttg catctcgacg aaattctcaa
     8341 tgcctgatgt gatgcggcgt agactcatgt ctacgccgta ttaatagata atgccaatca
     8401 aaataattgc tacaaatttg taacttttgc tgttgtacct gtacaatgtc ccggtgttca
     8461 agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt tattaacaat atttgccagg
     8521 gacttgtggt tttcatttag gcgtggcaat tctataatga tacgcattat ctcaagagca
     8581 aattctgtca cttcttctaa tgaagtgaac cgcttagtaa caggacagat tccgcatgac
     8641 tgataacaat accgccctaa agaaagctgg cctgaaagta acgcttcctc gtttaaaaat
     8701 cctggaagtt cttcaggagc cggacaacca tcacgtcagt gcggaagatt tatacaaacg
     8761 tctgatcgat atgggtgaag aaattggtct ggctacggta tatcgcgtac tgaaccagtt
     8821 tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc ggtaaatccg tatttgaact
     8881 gacacagcaa catcaccacg atcacctgat ctgcctcgac tgcggcaagg ttatcgaatt
     8941 tagtgatgat tccatcgaag cgcgtcagcg tgaaattgcc gcaaaacatg gcattcgcct
     9001 gactaaccac agtctctatc tttacggtca ctgtgccgaa ggcgattgcc gcgaagatga
     9061 acatgcgcac gaaggcaaat aagccagcct gaatgagaaa aagccaacct gcgggttggc
     9121 ttttttatgc aagggaaata agaaaagcga gagttacagc tctcacttat ttgttattac
     9181 tgcgaatttc ctgccaaact ttatcacaat cggctttcac cgcctgatca tttccggttt
     9241 gcgttgcacg cagacactgc tgataatcca gtacgcggac actttcctgg tcggcaaaat
     9301 gctgatgttg tttctctttc ttcagcacgt ttaacacgct ctggcaggct tcaatttttt
     9361 ctggcgaacc ttgtgcggtg ttgatacagg cgctgtatgc ctctttcaga cggctgtctt
     9421 cctgtggcgc ggttgattgc gcacaagcca ccagccccga tgccatcatg gcgatgagaa
     9481 tcagtttttt catagcaaat tcctcaaatg tgcggcaggc acaaaacctg ccgcgtcggg
     9541 catcagaaga tggtgaatgg tgcgattacc ataaatttca cgtcacgctc atcctggaag
     9601 atgttgccgt aaccaccgcc ccagcttggg atgtcggagt ggttgtcgta ttcggtgaaa
     9661 tgcagtttga acatcgtgcc tttggcgcga ccgtcctgaa tggtgtagac cgcatccagg
     9721 ctgtatgcag actcttcaat agtccggttt ttgtcgtagt acgcatccgg attgctctgc
     9781 caggtcgcag gtttagcatc ccatgcgtaa acgtaggaag cgccgatggc gaagcctgga
     9841 agattccagt ttttcaggtc atacatcgca ccgaagaaga ctgctttttc gccgttggcg
     9901 ttgaagtcag aacggttgtc ccaccagata tccaggcgac cgtttgagga agcgtaggtt
     9961 ggagtcatac gttgcaggaa gtatccctgc tgaccgtcag ccttaaccca ggtgccttcg
    10021 aggcgcaaat ccactacgtc agccgcccgg taaccaaagg tcaacgcctg cagccaggcg
    10081 gtgccgtcat aaaggtcgtt gacgctgcga tcgtcaactt tatcgcgggt accgtagaac
    10141 tggtagctgg tggttaacgg gctaccggcg atatcaaatt tgtagctggc tttggcaaaa
    10201 tattgatcga tatacccttc cgcctgacca aaagccgctt ccagtacgaa gttatttttg
    10261 aagtcgtatt tcgccccaag ggagtgcaga taatcaactt tggtggtttt atcgttctga
    10321 taaaactcat ccatttccag atgccacggc gctttgtatt cgttggtcca catgtaggag
    10381 aaactcaacg caccggcatc gccgtaatca aaattcgctc cggcttccgc accctgataa
    10441 gtacccggca taaagctcca gtgaggcgct aacagcgtct gaccggttgg ctgaatataa
    10501 cctgccctcg cccaaaccgg accgtattta aatttggccg ctgctttata caggcttata
    10561 ccgcttttgt cgccggacca gtcttcgtca taggctttat tactttttga aaacgcgatt
    10621 tcgttcgggt gagagctgtc gccgttttcc gccatttcaa tcgccgtaaa cgcggcaata
    10681 tcaaggccga acatatcagc agcataacca gactgaaaat cgaggttggc attccaggtg
    10741 gagtgagaaa ggttggtttt gtatttgtcg ccgtcggtaa catctttacg gttgcgttca
    10801 cgctgccagt aatagatacc gccggttaag gttgaatcgt cgatgaatcc ttcggcgcga
    10861 gcctccggca ttgccataaa gcccgacatt gctgtaacac cggcgatagc cagcgccagc
    10921 gtactacgtt tgccactaaa cgtacgcatg ggttaatcct ctttgacgta taaattgctg
    10981 caccaaaggt gaacagcgaa aaaaaataaa aatttaaaaa gcagcgttga gaaaagtctc
    11041 gctgactaca ggaacagact atttttcgcg acgcgaatta tcaatctctt tctgtaacca
    11101 gaatgtaaga acatgagcaa ccgcacatat tttgatgatt tttgttacaa ggcttaagtt
    11161 tgcggttgaa aggcttgcag aagaataaaa gcgtgaattt ccagcaagat gaaagcggtt
    11221 acattttatt taattatgtt ttgttatttt aaatttattt tatggataaa ttgcgaagca
    11281 ggattaattc gcgtagaaaa gtagaaaaaa acgccacaga gcgcggcgtt ttggattgtt
    11341 tgctgtttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag cacgcaaact
    11401 caatatattg cagagatcat gtaggcctga taagcgtagc gcatcaggca atttagcgtt
    11461 tgaaactgtt tgtctgttcc ttgccggatg cgacgtaaac gccccatccg gcatagcgaa
    11521 acttaaaatt actcgcctac tttcgcccag gtatcacgca gcccaacggt gcggttaaat
    11581 accggttttt ccgccgtaga atggcggcta tcgaggcaga agtaaccttc acgctcaaac
    11641 tggaatgctt tacccgcaac cgcatctttc agcgacggtt cagcaaagcc ctgtttgatc
    11701 accagcgatt ccgggttaat caccgacagg aaatcatccg cagcacctgg gttaggcacg
    11761 ctgaacagac ggtcatacaa acggatttca accggcagcg catgtgccgc gctcacccag
    11821 tgaataacac ctttgacttt acgaccatct gccggatctt tgcttaaggt atcggcgtca
    11881 taagtacaga agatggtggt gatattacct tcggcatctt tctcgacgcg ttctgcctta
    11941 atcacataag cattacgcag acgcacttct ttacccagca ccagacgttt gtactgcttg
    12001 ttagcttctt cgcggaaatc ggcgcgatca atccaaatct caccgctaaa cggcacctga
    12061 cggctgccca tttccggttt gttcggatgg ttcggcatgg taaccatttc gccttcgccc
    12121 tgatagtttt cgataaccag tttcaccgga tcgataaccg ccattgcgcg cggcgcattt
    12181 tcgttgagat cttcacggat gcaggattcc agcgacgcca tctcaatggt gttgtcctgc
    12241 ttggtcacgc cgatgcgttt gcagaactca cgaatagaag ccgcagtgta accacgacga
    12301 cgcagaccgg aaatggtcgg catacgcggg tcatcccagc cttcaacgtg cttgtcggtc
    12361 accagcaggt tcaacttacg cttggacatc acggtgtatt ccagattcag gcgcgagaac
    12421 tcatactggc gcgggtgaac aggaatcgtg atgttgtcca gtacccagtc gtacagacga
    12481 cggttgtcct ggaactcaag cgtacacaga gagtgcgtaa taccttccaa ggcatcgctg
    12541 atgcagtggg tgaagtcgta catcgggtag atgcaccact tgttgccagt ctggtggtgt
    12601 tcagcaaatt taatacggta cagcaccgga tcgcgcatca cgataaacgg tgaagccatg
    12661 tcgattttcg cacgcaggca ggctttacct tcttcaaaac caccggcacg cattttttcg
    12721 aacagcgcca ggttctcttc aacgctgcgg tcgcggtacg ggctgttttt acccggttgc
    12781 gtcagggtgc cgcggtattc gcggatctgt tccggcgtca gttcatcaac gtacgccagg
    12841 cctttattga tcagttcgat cgcataggcg tggagctgat caaaataatc ggaggagtaa
    12901 cggacgttac cagaccagtg aaaacctaac cactctacgt cgtttttgat cgactcaaca
    12961 tactcgatat cttcttttac cgggttagtg tcgtcgaaac gcaggttgca ctggccttta
    13021 tagtcctggg cgatcccgaa gttcaggcag atagatttcg catggccaat atgcagatag
    13081 ccattcggct ccggcgggaa acgggtgtgt actgtggtgt gcttaccact ggccagatct
    13141 tcatcgatga tctgacggat aaagttagtc gggcgggctt ctgcctcact catcgtggat
    13201 tcctcaaagc gtaaacaacg tataacggcg tatgatctta taagcgggac aggctgacaa
    13261 ctgttagttt tttaaaattg ataatctaac aaggattatg gggatgattg atgaaaaaaa
    13321 aagcggcaga gaagatctcc accgcttttg ccagtcctgc gtgattgtcg gatgcaacgc
    13381 taaagcgtct tatccaacct ggactgagct tttgtaggcc tgataagacg cgtcaagcgt
    13441 cgcatcaggc atgaagcaga ttactttttg atttcataca gcggtgtttg acccgccaca
    13501 acatggccct gagctttaat gatcaagcca ctgaaatcgt cgatattgct gcaaaccacc
    13561 ggactaatca tcgagcgggc gttagcgttc aggtaatcca gatccatttc cagaatcggt
    13621 tgccctgctc ttacctgcgc gccctcttcc accagacgtt taaagccttt accttccagc
    13681 gctacggtgt cgatacccat gtggacgacg atctccgcgc ctttttcggt ttccagacag
    13741 aacgcgtggt tggtgttgaa gattttcacg attgtccctg cggctggtga tacgacgatt
    13801 ttatctgtcg gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca
    13861 ggaacctgat ccagtgccac gacatcaccg gtaatcggcg ataccagctc cgcgatagat
    13921 accgcgtttg gtacagcctg cggttttgct acaggagcgg cagttgccgg agttgcttca
    13981 gctgacgcag ctgctaccgg gccacgggca acgactttct tcatcgcatc gccaatggat
    14041 tctgctttcg cgccaacaat cacctgaatg gtctgtttgt tcagttttac tacaccagaa
    14101 gcacccagac gtttacacat cgcatcgttg acgcgagctg agtcagccac ggtaaggcgc
    14161 agacgggtaa tacaggcgtc aatcgctttc aggttgtcag taccgccaac cgcagcaata
    14221 tagttggttg ccagttgagt cagaccttct tcagtattgc tgttagcctc ttcagttacg
    14281 atctcgtctt ctttatcttc acgacccggc gttttcagat tgaacatgcg gataaccaaa
    14341 ctgaacacca cgaagtagat agcgaagaag acaacaccca taaccagcag catccagacg
    14401 ttctggctgg cggccggcag gttatacatc aacgcgtagt cgatagcacc cgcagagaaa
    14461 gagaagccag catggatacc cagcagcgtt gccacaaaca ggctgatacc ggtcagcagt
    14521 gcgtgcagga ggtacagcag cggtgcaagg aacatgaaca ggaattccag cggctcagtc
    14581 acaccggtca ggaacgcagt aacagcaaca gaaagcagca tcccgccaac catcggacga
    14641 cgctctttcg gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc gaacatcatg
    14701 atcgggaaga agccggacat gaacatcccc gcggtgccgt caccagcgta gaaacggttg
    14761 atgtcgccgt ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg gaaccaggcg
    14821 atggtgttca gcacctgatg cagaccggtt gggatcagca gacggttgat gaaaccaaag
    14881 ataccggaac ccagcgcgcc cgcagaaacg atccactcgc cgcctgcatg gatagcgtgc
    14941 tgtaccggcg gccagacgta accaaaaatg gccgccagca ccagacagaa gaagccggtg
    15001 gcgatcggca caaagcgttt gccgccgaag aagctcagga agtccggcag tttaatatcg
    15061 gaccaacggt tataggctgc gccaccaacc agaccggtaa tgatacccgc cagtacaccc
    15121 atgttaattt ctgggttgat ggtcaccatc gctttggtta acacaaagta acctaccgca
    15181 cccgccagcg ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac accgatggcg
    15241 aagattaatg cgaggttatc aaaaatcgca ccgcccgcct gggcaataaa cgcaacgtta
    15301 agtaaatctg gctgaccgaa tcgcagcaac agtgccgcca ccggcagcac cgcgataggg
    15361 agctgtaacg ccctaccgag tcgctggaaa aaacctaaaa tattcatctt attcccccta
    15421 cgagaaccct atttggctcg tttaaagccg tatttttatt ttgctgcaaa ttgtactgcc
    15481 gatgttctgt aatcagattg ttagatcatc tgctacagag tgtgtgaaaa tttaattcgt
    15541 atcgcaaatt aaatgcgtgt cttttgtgag ttttgtcacc aaatatcgtt atcatcactc
    15601 ccttttactg gctaaaccag aaaacttatt ttatcattca aaaaatcagg tcggattgac
    15661 gcctgtctgc gcaaatccag gttacgctta aagatgccta atccgccaac ggcttacatt
    15721 ttacttattg aggtgaataa tgagactgat ccccctgact accgctgaac aggtcggcaa
    15781 atgggctgct cgccatatcg tcaatcgtat caatgcgttc aaaccgactg ccgatcgtcc
    15841 gtttgtactg ggcctgccga ctggcggcac gccgatgacc acctataaag cgttagtcga
    15901 aatgcataaa gcaggccagg tcagctttaa gcacgttgtc accttcaaca tggacgaata
    15961 tgtcggtctg ccgaaagagc atccggaaag ctactacagc tttatgcacc gtaatttctt
    16021 cgatcacgtt gatattccag cagaaaacat caaccttctc aacggcaatg ccccggatat
    16081 cgacgccgag tgccgccagt atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt
    16141 tatgggcggt gtaggtaacg acggtcatat tgcatttaac gaaccggcgt cttctctggc
    16201 ttctcgtact cgtatcaaaa ccctgactca tgacactcgc gtcgcgaact ctcgtttctt
    16261 tgataacgat gtcaatcagg tgccaaaata tgccctgact gtcggtgttg gtacattgct
    16321 ggatgccgaa gaagtgatga ttctggtgct gggtagccag aaagcactgg cactgcaggc
    16381 tgcagttgaa ggttgcgtga accacatgtg gaccatcagc tgtctgcaac tgcatccaaa
    16441 agcgatcatg gtgtgcgatg aaccttccac catggagctg aaagttaaga ctttaagata
    16501 tttcaatgaa ttagaagcag aaaatatcaa aggtctgtaa ttgttatccc tgccctggct
    16561 ccttgctcag ggcaatattt tttaaaatcg ggggtcagaa tgtatgcatt aacccagggc
    16621 cggatcttta ccggccacga atttcttgat gaccacgcgg ttgttatcgc tgatggcctg
    16681 attaaaagcg tctgtccggt agcggaactg ccgccagaga tcgaacaacg ttcactgaac
    16741 ggggccattc tctcccccgg ttttatcgat gtgcagttaa acggctgcgg cggcgtacag
    16801 tttaacgaca ccgctgaagc ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat
    16861 gagaaatcag gctgtactaa ctatctgccg acgcttatca ccaccagcga tgagctgatg
    16921 aaacagggcg tgcgcgttat gcgcgagtac ctggcaaaac atcccaatca ggcgttaggt
    16981 ctgcatctgg aaggtccgtg gctgaatctg gtaaaaaaag gcacccataa tccgaatttt
    17041 gtgcgtaagc ctgatgccgc gctggtcgat ttcctgtgtg aaaacgccga cgtcattacc
    17101 aaagtgaccc tggcaccgga aatggttcct gcggaagtca tcagcaaact ggcaaatgcc
    17161 gggattgtgg tttctgccgg tcactccaac gcgacgttga aagaagcgaa agccggtttc
    17221 cgcgcgggga ttacctttgc cacccatctg tacaatgcga tgccgtatat taccggtcgt
    17281 gaaccgggcc tggcgggcgc gatcctcgac gaagctgaca tttattgcgg tattatcgct
    17341 gatggcctgc atgttgatta cgccaacatt cgtaacgcta aacgccttaa aggcgacaaa
    17401 ctgtgtctgg ttaccgacgc caccgcgcca gcaggtgcca acattgaaca gttcattttt
    17461 gcgggtaaaa caatatacta ccgtaacgga ctttgtgtgg atgagaacgg tacgttaagc
    17521 ggttcatcct taaccatgat tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca
    17581 ctggatgaag tgctgcgtat ggcgacgctc tatccggcgc gtgcgattgg cgttgagaaa
    17641 cgtctcggca cactcgccgc aggtaaagta gccaacctga ccgcattcac acctgatttt
    17701 aaaatcacca agaccatcgt taacggtaac gaggtcgtaa ctcaataaga gaaagtatga
    17761 caccaggcgg acaagctcag ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg
    17821 cggtttatcg cctgattgac cagtacgggc caatctcgcg gattcagatt gccgagcaaa
    17881 gccagcttgc ccccgccagc gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga
    17941 tcaaagaagt tgatcagcag gcctccaccg ggggccgccg cgctatctcc atcgtcaccg
    18001 aaacccgcaa tttccacgca atcggcgtac ggcttggtcg tcatgacgcc accatcactc
    18061 tgtttgatct cagcagcaaa gtgctggcag aagaacatta cccgctgccg gaacgtaccc
    18121 agcagacgct ggaacatgcc ctgctgaatg ccattgctca gtttattgat agctaccaac
    18181 gcaagctgcg cgagctgatc gccatttcgg tgatcctgcc agggcttgtt gacccggaca
    18241 gcggcaaaat tcattacatg ccgcatattc aggtagaaaa ctgggggctg gtagaagcac
    18301 tggaagaacg ttttaaagtg acctgtttcg ttggtcacga tatccgtagt ctggcgctgg
    18361 cggagcacta cttcggtgca agtcaggatt gcgaagactc cattctggtg cgtgtccatc
    18421 gcggaaccgg ggccgggatt atctctaacg ggcgcatttt tattggccgc aacggcaacg
    18481 tcggtgaaat tggccatatt caggtcgaac cgctgggtga acgctgccac tgcggcaact
    18541 ttggctgcct ggaaactatc gccgccaacg ctgccattga acaacgggtg ttgaatctgt
    18601 taaagcaggg ctaccagagc cgtgtgccac tggacgactg caccatcaaa actatctgca
    18661 aagccgcgaa caaaggcgat agcctggcct cggaagtgat tgagtatgtc ggtcgtcatc
    18721 tgggcaaaac catcgccatt gctatcaact tatttaatcc gcaaaaaatt gttatcgctg
    18781 gtgaaatcac cgaagccgat aaagtgctgc tccctgctat tgaaagctgc attaataccc
    18841 aggcgctgaa ggcgtttcgc actaatctgc cggtggtacg ttctgagctg gatcaccgct
    18901 cggcaatcgg cgcttttgcg ctggtaaaac gcgccatgct caacggtatt ttgctccagc
    18961 atttgctgga aaattaatgt gcttttatag tggcgcttat tgttgtcaat attctgggta
    19021 gtccatgacc attaaaaatg taatttgcga tatcgacggc gtgctgatgc acgataacgt
    19081 cgccgtaccg ggtgcagcgg aatttttgca cgggattatg gataaaggcc tgccgctggt
    19141 gttgctgacc aactatcctt cgcagaccgg gcaggatctg gcgaaccgct ttgccactgc
    19201 cggtgtcgat gtacctgaca gcgtgtttta tacctctgcg atggcgaccg ccgatttcct
    19261 gcgtcgccag gaaggcaaga aagcgtatgt ggtgggcgaa ggcgcactga ttcatgaact
    19321 gtacaaagcc ggtttcacta ttaccgatgt gaaccctgat tttgtgattg ttggcgaaac
    19381 gcgttcctac aactgggaca tgatgcataa agcggcctat ttcgtcgcta acggtgcacg
    19441 ttttatcgcc accaacccgg acacccacgg gcgcggtttt tatcccgcct gtggcgcgtt
    19501 gtgtgcaggg atcgaaaaaa tctccgggcg taaaccgttc tatgttggta agccgagtcc
    19561 gtggatcatc cgcgcggcat taaacaaaat gcaggcgcat tcggaagaaa cggtgattgt
    19621 cggcgataac ctgcgtaccg atattctggc cggcttccag gcaggtctgg agacgattct
    19681 ggtgctttct ggtgtttcgt cgctcgacga tatcgacagt atgcctttcc gccccagctg
    19741 gatttacccg tcagtcgctg aaatcgacgt tatctgaata gcaaccacgt ctcaggacgt
    19801 ggttggctct gatgacaaac gccaaactgc ctgatgcgct acgcttatca ggcctacgca
    19861 gctcctgcaa tatattgaat tttcatgctt ttgtaggccg gataaggcgt tcacgccgca
    19921 tccggcatga acaaatcgca ctttgtcagc aatttggccc ccgtaaaata taaacattca
    19981 ataaaacccg ctaaaaatta tcgattcatc aataaatata tcctcacacc agaccttttt
    20041 tcaccaatcg acaatcacca ttacgttttt tattttttca ccgccaaatc gcttgcgcac
    20101 agtgctgctt tgcggcattt ttttaaacaa gcaaacacaa caagcaacaa atacaaggtt
    20161 aacggagaag gttatgtgtt caatttttgg cgtattcgat atcaaaacag acgcagttga
    20221 gctgcgtaag aaagccctcg agctgtcacg cctgatgcgt catcgtggcc cggactggtc
    20281 cggtatttat gccagcgata acgccattct cgcccacgaa cgtctgtcaa ttgttgacgt
    20341 taacgcgggg gcgcaacctc tctacaacca acaaaaaacc cacgtactgg cggtaaacgg
    20401 tgaaatctac aaccaccagg cattgcgcgc cgaatatggc gatcgttacc agttccagac
    20461 cgggtctgac tgtgaagtga tcctcgcgct gtatcaggaa aaagggccgg aatttctcga
    20521 cgacttgcag ggcatgtttg cctttgcact gtacgacagc gaaaaagatg cctacctgat
    20581 tggtcgcgac catctgggga tcatcccact gtatatgggg tatgacgaac acggtcagct
    20641 gtatgtggcc tcagaaatga aagcgctggt gccagtttgc cgcacgatta aagagttccc
    20701 ggcggggagc tatttgtgga gccaggacgg cgaaatccgt tcttactatc atcgcgactg
    20761 gttcgactac gatgcggtga aagataacgt gaccgacaaa aacgagctgc gtcaggcact
    20821 ggaagattca gttaaaagcc atctgatgtc tgatgtgcct tacggtgtgc tgctttctgg
    20881 tggtctggat tcctcaatta tttccgctat caccaagaaa tacgcagccc gtcgcgtgga
    20941 agatcaggaa cgctctgaag cctggtggcc gcagttacac tcctttgctg taggtctgcc
    21001 gggttcaccg gatctgaaag cagcccagga agtggcaaac catctgggca cggtgcatca
    21061 cgaaattcac ttcactgtac aggaaggtct ggatgccatc cgcgacgtga tttaccacat
    21121 cgaaacttat gatgtgacca ctattcgcgc ttcaacaccg atgtatttaa tgtcgcgtaa
    21181 gatcaaggcg atgggcatta aaatggtgct gtccggtgaa ggttctgatg aagtgtttgg
    21241 cggttatctt tacttccaca aagcgccgaa cgccaaagaa ctgcatgaag agacggtgcg
    21301 taaactgctg gccctgcata tgtatgactg cgcccgtgcc aacaaagcga tgtccgcctg
    21361 gggcgtggaa gcgcgcgttc cgttcctcga taagaaattc cttgatgtgg cgatgcgtat
    21421 taacccacag gataaaatgt gcggtaacgg caaaatggaa aaacacatcc tgcgtgaatg
    21481 ttttgaagcg tatctgcctg caagcgtggc ctggcggcag aaagagcagt tctccgatgg
    21541 agtcggttac agttggatcg acaccctgaa agaagtggcg gcgcagcagg ttaccgatca
    21601 gcaactggaa actgcccgct tccgcttccc atacaacacg ccaacctcta aagaagcgta
    21661 tctgtaccgg gagatctttg aagaactgtt cccgcttccg agcgccgctg agtgcgtgcc
    21721 tggcggtcct tccgtcgcgt gttcttccgc taaagcgatc gaatgggatg aagcgttcaa
    21781 gaaaatggac gatccatctg gtcgtgcggt tggtgttcac cagtcggcat ataagtaaga
    21841 aatgcgaaac agccccggtg aaatccgggg ctgtttcagt tattgaggtg cttgttgatt
    21901 ctgatatgca gtaaggaaat gttccgggtt tgccacatca ctgtttgaca actcatcact
    21961 gtaggccagt atttcataga ttaatttccc accactattg ttgaattccg aatttcatcc
    22021 tgtatgacaa aagaatggct gtagggtggg aaaaaacaca aagtaaacaa taattgacga
    22081 atatagcgcc acgctgttcg caacctaacc aaacagtcac tttcgagcaa ttttccttga
    22141 aaaagaggtt gacgctgcaa ggctctatac gcataatgcg ccccgcaacg ccgataaggt
    22201 atcgcgaaaa aaagatggct acgtagctca gttggttaga gcacatcact cataatgatg
    22261 gggtcacagg ttcgaatccc gtcgtagcca ccatcttttt tgcgggagtg gcgaaattgg
    22321 tagacgcacc agatttaggt tctggcgccg caaggtgtgc gagttcaagt ctcgcctccc
    22381 gcaccattca ccagaaagcg ttgtacggat ggggtatcgc caagcggtaa ggcaccggtt
    22441 tttgataccg gcattccctg gttcgaatcc aggtacccca gccatcttct tcgagtaagc
    22501 ggttcaccgc ccggttattg gggtatcgcc aagcggtaag gcaccggttt ttgataccgg
    22561 cattccctgg ttcgaatcca ggtaccccag ccatcgaaga aacaatctgg ctacgtagct
    22621 cagttggtta gagcacatca ctcataatga tggggtcaca ggttcgaatc ccgtcgtagc
    22681 caccaaattc tgaatgtgtc gaatacgttc ggtaaattca aaaaccaatt tgttggggta
    22741 tcgccaagcg gtaaggcacc ggattctgat tccggcattc cgaggttcga atcctcgtac
    22801 cccagccaat ttattcaaga cgcttacatc gtaagtgcac ccagttgggg tatcgccaag
    22861 cggtaaggca ccggattctg attccggcat tccgaggttc gaatcctcgt accccagcca
    22921 cattaaaaaa gctcgcttcg gcgagctttt tgcttttctg cgttcattca atgtcgaatg
    22981 cgatgttgac acgtcttatc cttcaatgtc ggatgcgacg ctgccgcgtc ttatccgacc
    23041 tacggttggc acgcatccgg caatgttgta aggctacaac cctaacgcat atttcagcgc
    23101 ctgacgtttc aacacgccag cacgctccgc cgccattaac ccgagattac gcataaaacg
    23161 cagtggtggc agattattgc tgaatccggc ataaaacaga tccataccgc tttgcataat
    23221 gaagttatcc gccatgcgcc gcatctggta acgcttgagg attggataac tggcccacgc
    23281 ttcgccgtag ctgcgggcgt tgaccagaac atcaatcagg gcatcgacat cacgataacc
    23341 aagattcacc ccctgccccg ccagcggatg gatggtatgc gcggcatcgc ccaccagcgc
    23401 aagccctggc tgcacatact gcaacgcatg gcgacgcgtc agcggaaacg caccagcggc
    23461 aagcggtgta acgtaaccca gacgcgacgg gaaatgcttc gcgatttccg cctgtaactg
    23521 cgccatgttc atattctgca actggcgaat acgcgccgga gagtcatacc acaccagcga
    23581 tgcccagtta tcaaacaacg gcagaaacgc acgcggtccg tccggagtaa attgctgcca
    23641 ggtgctgtcg ccgggatcgt tctcacactg tacgctaatc aacatacacg actgcgcata
    23701 ctgccaggca tgaacgccaa tacccgccat ctgccgcacc tgcgaatttg cgccgtcggc
    23761 accaatcacc agcttcgcgc ggatcgtttc tccacccttc agttccagct cctgaagatc
    23821 atcatggcga tgcagcgcaa tcagtgagcc tggcacacgt aacgttactt tcggatgcgc
    23881 ttccagcgcc tgccacagcg cctgttgcag gacggtgttt tccaccatat agccaagcaa
    23941 cggtagctta agttcagcgg cgtcaaacac cacatgcgcc gtttcccact cccacgtttc
    24001 cagtctgcgg taaggatggc aacgcatagc ctgtactgca tcccagaccc ctaacccttt
    24061 aagcaatgat accgaagccg cgctgatcgc cgagatccgc acatccggtt ggctatcagc
    24121 gacaaacggt gcaggttctg cgtgctcgat caccgttacc gtaaatccgt gctgtgccag
    24181 ccccagcgcc agtgcgccgc cgaccattcc tacgccgaca atggcaattt ccgttggttg
    24241 atttgtcatg gtcaatcatc ctgttagcaa tatgctttaa gtttacagga tttttacccc
    24301 caagggctga taacgctcat actggtcaca acggcagcaa agcattacac tatgcgccct
    24361 gcattcctgg ctactatttc gcaagagcaa gtcgatgacc aaaaaactcc atattaaaac
    24421 ctggggctgt cagatgaacg agtacgattc atcgaagatg gccgatctgc tggatgccac
    24481 ccacggctat caactgaccg acgtggcgga agaagcggat gtgctgctgc tgaacacctg
    24541 ctcaatccgc gagaaggctc aggaaaaagt cttccatcag ttgggtcgct ggaaactgtt
    24601 aaaagagaag aatccagacc tgattatcgg cgtcggtggc tgcgtggcat cgcaagaagg
    24661 cgagcacatt cgccagcgcg cccactatgt cgatattatt tttgggccgc aaacgctgca
    24721 tcgtctgccg gagatgatca actccgtgcg cggcgaccgc agcccggttg tagatatcag
    24781 cttcccggaa atcgagaagt ttgaccgtct gccggaaccg cgcgccgaag ggccgaccgc
    24841 gtttgtctcc atcatggaag gctgcaataa atattgcacc tactgcgtgg tgccttacac
    24901 ccgtggtgaa gaggtaagcc gtccgtccga cgatattctg tttgaaattg cccagcttgc
    24961 ggctcagggc gtgcgtgaag tcaacctgct cggtcagaac gtgaacgcct ggcgtggtga
    25021 aaactacgac ggcaccactg gatcgtttgc cgatctgctg cgtctggttg cggcaatcga
    25081 cgggatcgat cgtattcgct ttaccaccag ccatccgatc gaattcaccg acgatatcat
    25141 cgaagtgtat cgcgacacgc cggagctggt gagcttcctg catctgccgg tacagagcgg
    25201 ttccgatcgc attctgaacc tgatgggccg tacccatacg gcgctggagt acaaagcgat
    25261 catccgtaaa ctgcgtgcgg cgcgtccgga tattcagatc agttctgact tcatcgttgg
    25321 cttccctgga gaaaccaccg aagacttcga gaaaacgatg aagctgattg ccgacgtcaa
    25381 tttcgacatg agctacagct ttatcttctc tgcacgtcca ggaacgcctg ccgctgatat
    25441 ggttgatgat gttccggaag aagagaagaa gcagcgtctg tatattctgc aagaacgtat
    25501 caatcagcaa gcgatggcat ggagccgtcg gatgctcggc accacccagc gtattctggt
    25561 agaaggtaca tcgcgtaaga gcatcatgga gctttccggg cgtaccgaaa ataaccgtgt
    25621 ggtcaacttc gagggcacac cggatatgat cggtaaattc gtcgatgtag aaattaccga
    25681 cgtctacccg aactctctgc gcggtaaagt ggtgcgtact gaagatgaaa tgggtctgcg
    25741 cgtggcagaa acacccgaat cggtgattgc ccgtactcgc aaagaaaatg accttggcgt
    25801 gggttattat cagccgtaat tctcaggccc gccgttccgg tgggccttgt tttttctctc
    25861 tttatcccca tctttatcgc aatgcttgcg cctttttcct gtggcgtgaa taatttcctt
    25921 ataggccggt gaatgttcag cttccggcgg catacagata agaggaacgg tttgaacata
    25981 gacactcgcg aaatcaccct ggagccagca gacaatgcgc gtctgttgag cctgtgcggc
    26041 ccgtttgatg acaacatcaa gcagctcgaa cgtcgtctcg gcatcgagat caatcgccgc
    26101 gataaccact ttaaactgac cggccgtccg atttgcgtca ccgctgcggc agacattctg
    26161 cgtagcctgt atgtcgatac tgccccgatg cgcggtcaga ttcaggatat cgaaccggaa
    26221 cagatccacc ttgcgattaa agaagcgcgg gtactggagc aaagcgcgga gagcgtgccg
    26281 gagtacggca aagcggtcaa tatcaaaact aaacgcggcg taattaagcc gcgcacgcca
    26341 aaccaggcgc agtacatcgc caatattctc gaccatgaca ttaccttcgg cgttggcccg
    26401 gcgggtacgg gtaaaaccta cctggcagtg gctgcggcag ttgatgccct ggagcgtcag
    26461 gaaattcgcc gtattctgct gactcgtccg gcagtagaag ccggtgagaa actgggcttc
    26521 ctgcctggcg atttaagcca gaaagtagac ccgtatctgc gcccactgta cgacgcgctg
    26581 tttgaaatgc tgggctttga gaaagtcgag aaactgattg agcgcaacgt tattgaagtc
    26641 gcgcctctgg cctatatgcg tggtcgtacg ctgaatgacg cctttatcat tctcgatgag
    26701 agccagaaca ccaccatcga acagatgaag atgttcctga cccgtatcgg ttttaactca
    26761 aaagcggtta tcaccggcga cgtcacacag atcgacctgc cgcgtaatac taaatcaggt
    26821 ttgcgtcatg ccatcgaagt gctggccgat gtcgaagaga tcagctttaa cttcttccac
    26881 agcgaagacg tggttcgtca cccggtggtg gcgcgtatcg ttaacgctta tgaagcctgg
    26941 gaagaagcag aacaaaaacg aaaagcggcg ctggcggcag aacgcaagcg cgaagaacag
    27001 gaacaaaaat gagtcaggtg atcctcgatt tacaactggc atgtgaagat aattccgggt
    27061 taccggaaga gagccagttt cagacatggc tgaatgcggt gatcccgcag tttcagaaag
    27121 aatcggaagt gactattcgc gtggtcgata ccgccgaaag ccacagtctg aatctgacct
    27181 atcgcggtaa ggataagccg accaacgtgc tctccttccc gtttgaagtg ccgcctggca
    27241 tggagatgtc gctactgggc gatctggtta tctgccgtca ggtggttgag aaggaagctc
    27301 aggagcaagg caaaccactg gaggcgcact gggcgcatat ggtggtgcac ggcagtctgc
    27361 atttgttagg ttacgatcac atcgaagatg acgaagcaga agaaatggaa gccctcgaaa
    27421 cagagattat gcttgctctg ggctatgagg atccgtacat tgccgagaaa gaataatttg
    27481 ccaggcattt gactggcaga cgtccccgcc gttggtgatt aacgacggcg ttttgattaa
    27541 ctaacatgag aacccatacg acgccatgag cgacgacaat tcacacagta gtgacacgat
    27601 aagcaacaag aagggatttt tctccctgtt actcagccaa cttttccacg gtgaaccgaa
    27661 aaaccgtgac gaactgctgg cgctgatccg tgattccggg cagaacgacc ttatcgacga
    27721 agatacgcgc gatatgctcg aaggggtgat ggacatcgca gaccaacgcg ttcgcgacat
    27781 catgatcccc cgctcccaga tgattaccct gaaacgcaac cagacgctgg acgaatgcct
    27841 tgatgtcatc atcgagtccg cccactcacg tttcccggtg attagcgaag acaaagatca
    27901 cattgaaggg attctgatgg cgaaagactt gctgccgttt atgcgcagcg atgctgaagc
    27961 cttcagcatg gacaaagtgt tacgtcaggc ggttgtcgtt cctgaaagta agcgcgtaga
    28021 ccggatgctg aaagagtttc gctctcagcg ttaccacatg gcgatcgtca ttgacgaatt
    28081 cggtggggtt tccggtctgg tgaccattga agacatcctg gaactaattg ttggtgagat
    28141 tgaagacgag tatgacgaag aagatgatat cgacttccgt cagctgagtc gtcatacctg
    28201 gaccgtgcgc gcactggctt ccattgaaga cttcaacgaa gcgttcggca cccactttag
    28261 cgatgaagag gttgacacta tcggtggtct ggtgatgcag gcatttgggc atcttccggc
    28321 gcgtggcgaa actatcgaca tcgacggtta ccagttcaaa gtggcgatgg ccgacagtcg
    28381 gcgtattatt caggttcatg tcaaaatccc ggatgactca ccccagccaa agctggatga
    28441 ataaaaccga aactggatag ataactacat ggcttttgcc tcattaattg aacgccagcg
    28501 cattcgcctg ctgctggcgt tattattcgg tgcctgcgga acgctggcct tctctcctta
    28561 cgacgtctgg cctgcggcga ttatttcgct gatggggctt caggcgttga cctttaaccg
    28621 tcgcccactc cagtctgccg ctattggctt ttgctgggga tttggcctct ttggcagcgg
    28681 tattaactgg gtctatgtca gcatcgcgac ctttggcggg atgcctggcc cggttaacat
    28741 cttcctggtg gtgctgctgg cggcgtattt gtcgctgtat accggactgt ttgctggcgt
    28801 gctgtcgcgt ctgtggccga aaaccacctg gctgcgcgtg gcgattgccg ccccttctct
    28861 ctggcaagtg accgagtttc tgcgcggttg ggtactgaca ggcttcccgt ggttacagtt
    28921 cggctatagc cagattgatg gcccgttaaa agggctggca ccgataatgg gcgtggaagc
    28981 cattaacttc ctgctgatga tggttagtgg cctgctggca ctggcgttgg tcaaacgcaa
    29041 ctggcgtccg ctggtggtgg ccgtcgtgct gtttgcccta cccttcccgc tgcgttacat
    29101 ccaatggttt accccacaac cggagaaaac cattcaggtt tcgatggttc agggcgatat
    29161 tccgcaatca ctgaaatggg atggagatca gcttcttaat acgctgaaga tttactacaa
    29221 cgcaacggct ccgctgatgg gcaaatcatc gttgattatc tggccggagt cggcgataac
    29281 cgatctggaa attaatcagc aaccattcct caaagcactg gacggtgagt tgcgcgataa
    29341 aggtagctcg ctcgtaaccg ggattgtcga cgcgcgtctc aataagcaga accgctacga
    29401 tacctacaac accatcatca cactgggtaa aggtgcaccg tacagctacg aatcagccga
    29461 tcgctataac aaaaaccatc tggtgccgtt tggcgagttt gtcccgctgg agtcgattct
    29521 gcgtccgtta gcaccgttct ttgatctgcc gatgtcgtcg ttcagccgtg ggccatatat
    29581 ccagccgccg ctgtcggtaa atggtattga gcttactgcg gccatttgct acgagatcat
    29641 tcttggcgag caagtgcgcg ataacttccg cccggatacc gactatctgc tgactatctc
    29701 caacgatgcg tggtttggta aatctattgg tccatggcaa cacttccaga tggcgcgaat
    29761 gcgtgcgctg gagctggcgc gcccactgtt gcgcagcacc aacaacggca ttacggcggt
    29821 gattggcccg cagggtgaga ttcaggcgat gatcccgcag ttcacccgcg aggtgttgac
    29881 cactaacgtg acgccgacca ccggactcac accgtatgca cgaaccggca actggccgct
    29941 gtgggtgctg acggcactgt ttggttttgc cgctgtgctg atgagtctgc gagcacgtaa
    30001 acgttgatgt gcctgatgta tcctgcatga gcaaaacgta ctttctcatt gagctttggg
    30061 gcatggttat catgcccctt tttcttacgg ttcagggata caagccacat tgccatattg
    30121 gggatgacga ataattgcct gcggatcaag cgagcgcgtc ctgtccagtt ggatcaaccc
    30181 gttcttcgcc ataacaaata tttttctacc atccgctgaa aaacgaaggt gattaacgta
    30241 gctggcagga taaacgccaa taatctgccc ggcattaata ttgagcagga tgacataacc
    30301 ttgcaggcca tatgacacca acgctaaata ctgaccgtcc gggctaatac cctcaacgga
    30361 ataaacgctc ctgctacacc attgcgtcat aatgctggct tgttgatccc ctacgcttat
    30421 acgtgaaatc ccctggctgt cggcaaaaat tacatccccg ttttcaggtc gaaaacgcat
    30481 cgccgatatc gttcctcgcg catcactagc cggatagctt tctttctgat gcgtgatggt
    30541 attgatgcgt acaatgtcac ctctgaccat accgggtatc aacaatattt tttcatccgg
    30601 ggaaagagcc ataacccagg caccaggttt attactcgtc cccgtcccgt cgtatttctc
    30661 cagcgggata tccgccacat gctgataagt caccagatca atcgctgaca cactattact
    30721 ggtgcgattc atcacccagg cttttgtgcc atcacgcgag aggataaggc tgtcaggatg
    30781 tgcaccggtg gtaatcgact gaataatctc ccctgtcgcc acgttaatga ccgataacgc
    30841 attcgcttca aatgaagtcg cataaatcaa cttttcatcc gggcttaatg ccagcgatct
    30901 gacgccctga tctttacagc caccacaatg tttgactgac ttttctttcg ctatgggcgc
    30961 tgtaaaggta tgcaggattt tcttttgtcc gaggtcgaaa acaacaatgc tgttcgtgtt
    31021 ttcaattgcg gcatacaaca tattaccatc ccgactgatc gccatatcat ggatatcggc
    31081 aatctcagcg agcaaatcac cactggcctg ttcacgactt accggagcgg ataatgtttc
    31141 cctgagggcg ttgcgttcct ccgctaactg ctgctttttt tgccgtcgct cttgttcaat
    31201 ctcctgtgga taaaacgctt cacgcaggct ttgcctgact tgtaaaactg actcatccat
    31261 ctgctgctgc gcaagccaga tagccccggt cagaagaaga ctgcccgcaa caataatccc
    31321 gccttccgta acgagtcggt tttgcccaga taaacgcgcg ctcagccaac ccaacgcgcc
    31381 acccgcaatc aggccgccaa tgtgacaagc attatctatt cctgtttgcc gggcaccata
    31441 caacagtgtc agcgccacca tacccaacag agaaaatact cgcctctggt ttttatggta
    31501 tgtgcccgca cctgaagcaa actgtgttgc tatcgctgca ccagctattc ccagaatcgc
    31561 cccggatgcc ccaacgctga tgactatctg ctcactttcg cgtaacgcat aacaggcgct
    31621 gatgagccca ccaaagacgc cggagaataa ccaggtaatc aggagtcgcc atttccccag
    31681 aatctgttcc gccacgcctc ccacgaccag gaatgcaaac atattcatca gcagatgaga
    31741 aacactactg tgcagaaaga tactgctcaa taagcgccag ggttcacccg acaaagtaag
    31801 aggcgcaacg tttgcgccga gatggataag tgcggattga tcgacctctt cataactcat
    31861 ccattgtgcg agataacaaa atatcccaat acagagtgca aaaaggataa gggttattgc
    31921 gggtaattga acatttaacg gcttaaccga agatgcagac ataaggtgtt tcttccttga
    31981 aaagacaggt aaaaagcatt tttcccgtgt gcagttatca tcccggcaaa cgtcccatgc
    32041 cgaaaagata attctgtccg aaatatgact ttttcagcct aaaattacaa tcgtaattaa
    32101 ttcgccccta agtatttttt ggcatgtaag ttgctttatt tactgtgtaa gcgatgaaat
    32161 cagccgcgac gcaaccaggt tgcaccacca caaatcagcg gtagcaccaa aaaggtgcgg
    32221 tttaagaatt ttgcttcgga atggtgcggc gcatctcgca aaaacaaaca ataaaacagc
    32281 aatatcttta catttagcca aactaaatgt tacctaacag gcacaacact gcacaataaa
    32341 gttgcagacg ataacaacac aaacactcac aacgggtatc catgcgttct taacgcagaa
    32401 gataaaggag ttggatatgc aattacgtaa acctgccaca gcaatcctcg ccctggcgct
    32461 ttccgcagga ctggcacagg cagatgacgc cgccccggca gcaggcagta cgctggacaa
    32521 aatcgccaaa aacggtgtga ttgtcgtcgg tcaccgtgaa tcttcagtac ctttctctta
    32581 ttacgacaat cagcaaaaag tggtgggtta ctcgcaggat tactccaacg ccattgttga
    32641 agcagtgaaa aagaaactca acaaaccgga cttgcaggtg aaactgattc cgattacctc
    32701 acaaaaccgt attccactgc tgcaaaacgg cactttcgat tttgaatgtg gttctaccac
    32761 taacaacgtc gaacgccaaa aacaggcggc tttctctgac actattttcg tggtcggtac
    32821 gcgcctgctg accaaaaagg gtggcgatat caaagatttt gccgacctga aagacaaagc
    32881 cgtagtcgtc acttccggca ctacctctga agttttgctc aacaaactga atgaagagca
    32941 aaaaatgaat atgcgcatca tcagcgccaa agatcacggt gactctttcc gcaccctgga
    33001 aagcggtcgt gccgttgcct ttatgatgga tgacgctctg ctggccggtg aacgtgcgaa
    33061 agcgaagaaa ccagacaact gggatatcgt cggcaagccg cagtctcagg aagcctacgg
    33121 ttgcatgttg cgtaaagacg atccgcagtt caaaaagctg atggatgaca ctatcgctca
    33181 ggtgcagacc tccggtgaag cggaaaaatg gtttgataaa tggttcaaaa atccaattcc
    33241 gccgaaaaac ctgaacatga atttcgaact gtcagacgaa atgaaagcac tgttcaaaga
    33301 accgaatgac aaggcactga actaattaca agaaccaggg gcggaaattc cagccctctc
    33361 gattgttacg tagcacggac agactatacg cctgatggtc gttccccatc gggcctgaaa
    33421 accgcaatac gctgggtaac aatcttcgag ggtagcagtt aacgctgcta ccctcttttt
    33481 tctggagtag atttatgtct atagactgga actggggtat ttttttacaa caagccccgt
    33541 tcggcaacac cacctatctt ggttggatct ggagtggttt tcaggtgacg atcgctttgt
    33601 cgatctgcgc ctggattatc gctttcctcg tcggttcatt tttcggcatt ttacgtaccg
    33661 ttccaaaccg ttttctctcc ggtctgggta cgttgtatgt cgaactgttc cgcaacgtgc
    33721 cgttgattgt gcaattcttt acctggtatc tggtgatccc ggagctgctg ccggagaaaa
    33781 tcggcatgtg gtttaaggcc gagctggatc ccaatattca gtttttcctt tcatccatgc
    33841 tctgcctggg gctgtttacc gccgcccgtg tttgcgaaca ggtacgcgcc gctattcagt
    33901 cgctgccgcg tgggcaaaag aatgccgcgc tggcgatggg cctgacgcta ccgcaggctt
    33961 atcgatacgt actgctgcct aatgcttatc gcgttatcgt cccgccgatg acctcagaga
    34021 tgatgaacct ggtgaaaaac tccgccatcg cctcgactat tggtctggtg gatatggcgg
    34081 cgcaggcggg taaattgctt gattactcag cccacgcctg ggagtcgttt accgccatca
    34141 cgctggcgta tgtattgatt aacgctttta tcatgctggt gatgacgctg gttgaacgta
    34201 aagttcgcct gcctggcaac atggggggta aataatgtac gagtttgact ggagttccat
    34261 tgtcccttcc ctgccatatc tgctcgacgg gctggtgatc accctgaaaa tcaccgtcac
    34321 ggcagtcgtg attggtattt tgtggggcac catgctggcg gtgatgcgct tgtccagctt
    34381 tgcgcctgta gcgtggtttg ccaaagccta cgttaacgta ttccgctcga ttcctttagt
    34441 tatggttttg ctgtggtttt acctgattgt gccgggtttt ctgcaaaacg tgctgggatt
    34501 atcaccaaaa aatgatatcc gtttaatttc ggcgatggtg gcgttttcga tgtttgaagc
    34561 ggcctactat tcagagatta tccgtgctgg tattcaaagt atttctcgtg gtcagtcgag
    34621 cgccgcgctg gcgttgggaa tgactcactg gcagtcgatg aaactgatta ttctgccgca
    34681 ggcgttccgc gcgatggtgc cgctgctgct cactcagggc atcgtactgt tccaggatac
    34741 ctcgctggtg tatgtgttat ctctggccga tttcttccgt accgcctcaa ccattggtga
    34801 gcgtgatggt acgcaggttg agatgatcct gtttgccgga tttgtttatt tcgttattag
    34861 tcttagcgcg tcgttgttgg tcagctactt gaaaagaagg acagcataat gattaccctg
    34921 aaaaatgttt caaaatggta tggtcacttt caggtgctga ccgactgctc aaccgaagtg
    34981 aaaaaaggcg aagtggtggt ggtttgcggc ccgtctggtt ccggcaaatc aacgctgatt
    35041 aaaaccgtca acggcctcga accagtgcag caaggtgaaa tcaccgtcga tggtatcgtg
    35101 gttaatgaca agaaaaccga tctggcaaag ctgcgttccc gcgtcgggat ggtattccag
    35161 catttcgagc tgttccctca tctgtcgatt atcgaaaacc tgaccctggc gcaggtgaaa
    35221 gtgcttaaac gcgataaagc gccggctcgt gaaaaagcat tgaaattgct ggagcgcgtg
    35281 ggactttccg cacatgccaa taaatgtccg gcgcagcttt ccggcggtca gcagcagcgt
    35341 gtggcgatcg ctcgcgcatt gtgtatggat cctattgcga tgctgttcga cgaaccgacg
    35401 tcggcgctgg atccggagat gatcaacgaa gtgctggacg tgatggtgga actggcgaac
    35461 gaaggaatga ccatgatggt ggtgacccac gaaatgggct ttgcccgcaa agtggcgaat
    35521 cgggtgatct ttatggatga gggtaaaatt gtcgaagact cgccgaaaga cgctttcttc
    35581 gatgatccga aatcggaccg cgcaaaagac ttcctcgcga aaatcctgca ttaatcactc
    35641 tggcgcgcat acccaagtgc gcgccatctc acgctttatc gtttcgcccc tcgtatctgt
    35701 tacatcctgg cgttaccctt gtcgcaaaga agcacacaac aaggagcaac aatggcactg
    35761 ccaattctgt tagattgcga cccaggtcat gacgacgcta tcgcaatagt tctcgccctc
    35821 gcctcaccag agcttgatgt caaagcaatt acgtcttccg ccggaaacca gacaccagaa
    35881 aaaaccttac gcaatgttct tcgtatgctg accttgctca atcgcaccga tattccggta
    35941 gcaggcggcg cggtaaaacc gttaatgcgt gagttgatta tcgcggacaa tgtgcacggc
    36001 gaaagcggtc tcgacggccc ggcattaccg gaaccggcgt tcgcaccgca aaactgtacg
    36061 gcggtagagc taatggcgaa aacgctgcgt gaaagtgcgg aacctgtcac gattgtgtct
    36121 accggaccgc aaactaacgt tgccttgctg ctcaatagcc atccggaact gcatagcaaa
    36181 attgcccgta tcgtgattat gggcggcgca atggggcttg gtaactggac gcctgcggct
    36241 gaatttaata tttacgttga cccggaagcg gcagaaattg tcttccagtc agggatcccg
    36301 gtggtgatgg ctggtctgga tgttactcac aaagcacaaa tccacgttga agacactgag
    36361 cgtttccgcg cgattggcaa ccctgtttca accattgttg ccgaactgct ggatttcttc
    36421 ctcgaatatc ataaagacga aaaatggggc tttgtcggcg caccgctgca tgacccatgc
    36481 accatcgcct ggctgttgaa accggagttg tttaccactg ttgagcgctg ggttggcgtg
    36541 gaaacacagg ggaaatatac tcagggtatg acggttgttg attattatta tctgactggc
    36601 aataaaccga atgccaccgt aatggtcgat gttgatcgtc agggctttgt tgatttactg
    36661 gctgatcgtc tgaaatttta cgcttaacac acgtcattga ttaagatcac cacgcgttac
    36721 ctgggtggtc ttaatctgtt tatctctttt tctttcaaga tataaatgca atggataatg
    36781 cagaactcgc cattggtatc gatctcggta ctaccaatag tttaattgcc gtctggaaag
    36841 acggtgccgc gcaattaatt ccaaataagt tcggtgaata tttaacacca tccataatta
    36901 gcatggatga aaataatcat attttagtcg gaaaaccggc tgtatcacgg cgtacttcgc
    36961 atccggataa aacggcagcg ttatttaaac gtgcaatggg cagtaatacc aactggcggt
    37021 taggcagcga cacatttaac gcgccagaac tgtcctcttt ggtattacgc tcattaaaag
    37081 aagatgccga agaatttctg caacgtccga ttaaagatgt ggtgatctcc gttccggctt
    37141 atttcagcga tgaacaacgc aagcataccc gtttagcagc ggagttagcc gggttaaatg
    37201 cggtacgctt aattaatgaa cccacagcag ctgcgatggc gtatggcctg catacccaac
    37261 aaaatacccg ttcgctggtg tttgatctcg gtggcggcac gtttgacgtt acggtgcttg
    37321 agtacgccac gccggtgatt gaagttcacg cctccgctgg cgacaacttc ctcggtggcg
    37381 aagattttac ccatatgttg gtcgatgacg ttttaaagcg cgcggatgtc accaggacca
    37441 cgcttaacga gagtgaactg gcagccttgt atgcctgtgt ggaagcggca aaatgtagca
    37501 atcaatcgcc attacacatt cgctggcagt accaggatga agcacgggaa tgcgaatttt
    37561 atgagaatga actggaagat ttgtggttgc cgctgctcaa tcgcttgcga gtgccgattg
    37621 aacaggcgtt gcgcgatgcg cgtctgaagc caagtcaaat cgacagtctg gtactggttg
    37681 gcggcgcgtc acaaatgccg ctggtgcagc gaatcgccgt gcgtctgttt ggcaaattac
    37741 cgtatcaaag ttacgacccg agcaccattg tcgcgctggg cgcagcaatc caggccgcct
    37801 gccgcttacg cagtgaagat attgaagagg tgatcctcac tgatatttgc ccttactcgt
    37861 tgggcgttga agttaaccgc tagggcattt ccggcatttt ctcgccaatt attgaacgaa
    37921 acaccacagt accggtgtcg cgtgtagaaa cttactcgac catgcacccg gaacaggatt
    37981 ctattacggt taacgtctat cagggagaaa accacaaagt taaaaataac attctggttg
    38041 aatccttcga tgtgccgttg aagaaaaccg gggcttatca gtcgattgat attcgcttta
    38101 gttatgatat caacggcttg cttgaagttg acgtgcttct ggaagatggc agcgttaagt
    38161 ccagagtcat taatcatagc ccggtaacat tgagcgcgca gcagattgaa gagagtcgga
    38221 cgcggttatc cgcattgaaa atttatccgc gcgatatgct catcaaccgc acctttaagg
    38281 ccaaactgga agagttgtgg gcgcgggcgc tgggtgacga gcgagaagag attggccggg
    38341 tgatcaccga ttttgatgcg gcgttgcagt caaacgatat ggcccgcgtc gatgaggtca
    38401 ggcggcgggc gagcgtttat ttagccattg agacctcata atcacggctg ccggatgcgg
    38461 cgtaaatgct gcatccggca tcggcgcaaa aaacacatcg tcaactaaac aacatcgcga
    38521 ttttgccgag agcataacct ataaccagca ccgttaaact gattttgata atgctggtaa
    38581 tgctctgtgg tttttcaccc tcatcatttt tttcttcatg gttttcaggt gttggctcat
    38641 cgacatcttc atcctccggc tcgagtttta ctttcgtcaa cagcatccgg gtgataatgg
    38701 cctctaatgg atgagcatct ccgccttcca gccttgtatt aagacgggcc agcatctctt
    38761 gtttcggaaa cgcttcaagt actacctgtt ccgctacccg atacaaatcc agtaacggtg
    38821 aatcttcttc tgtctcgcta ccgactctcg atttccacca cgcccatgct ttgcgtttga
    38881 agaataacga ctcaataaac gattgaccta acggcgataa ttttccgcta tgtaaaattt
    38941 ccgcccataa cagccgcgtc cgggcgctct caccaggaac atatttcagc ggttgacctt
    39001 gcgcatctac catgctccgc gcttcgataa ccatcactaa taacgcgaaa taatccgggc
    39061 aatgacgctt gctccactgc aacaacaaat tatcagcttg tgtagatggg taactttccc
    39121 ggtacgcgca gagatcggca agcaggcttt ccccctcgcc gcagtacaac ctttgagcac
    39181 acaagtaata tcgggcatct tcgctttctg gttcttccat ttgccactgt ttggctacag
    39241 gaatgagttc cgccatacct gtttgcaccg agctgtacca gcgcaacagt ttgcgatgta
    39301 actttgcatc atcaggaatg aaccacggac cttccatcgc caaaaacgca gtgacgtagt
    39361 caggatggta acggcagcaa cgttccagcg cgaagtaata ctcaacagtc tggttctgca
    39421 acgcaacggg atattccaga aggctaagga aatcgaaaca atcaccagcc tgaatggcct
    39481 caagaaaagc ctccctttct tcctcatcca ttccttcgct gtcattaaac gaatgccagt
    39541 tcaaacgctc tgccagcaaa gaagcacaat ttaatgaaag atatcgcgct tctatagcga
    39601 ttgcacacag cggccagcgt aattgatcga catcctccat gttccaggta tttaattgct
    39661 gaataaattt ctgccaggcg gagggctgaa aacgatcact ttccgaatcc agtaatgtcc
    39721 tgaatgcacg cagtatttca tgttcagcgg cggcatcttt ttcttcatca tctgcttcct
    39781 cgacaggatt taccgccagt cgcagcgcct cttcataggc ctggcgtaat gctttaaacc
    39841 cctgcggatc ggtttcggga tgacataacg gtaagcgagc aagataagcc tggcggataa
    39901 tgtctatttg cgtcgtgctt tcaatttcga ggatttgcca acatgttttc ataggtcgct
    39961 cacatcctta agcgttaccg ggcattcagg ctggacagtt aatggctgcc agggaagcgt
    40021 ggcatagaaa cgatcgcttc ccgctgccag ctcttcgaac atccgactcc cggcgctatc
    40081 gtatttgtac agggttagcg cctcgcgtag cgaagtgttg tcttcgcact gagaagattg
    40141 ccagtagaga cgtcctaacg aataggcgga aaaatagtgc atccagctgt gatagtaatg
    40201 gtgagctcgt acatagacgc gcgattgcag ccataaactc tcttcttccg tcagccagtt
    40261 gttgagcaca ccgacccgtg acagaaaccc cattcgaaca taatcccagg ctttgattcc
    40321 cccttctcca cagcacatag cggtttgggc aacgaattgg gtataaatca accctctctc
    40381 cgagccacca gcaattaatg ctttccactc ttcaggggaa gagcgatgcc atgtcagata
    40441 taagccagct aaatcattgg catgaccatc atcagtcatc tgataaatag tctgaattaa
    40501 atcatcacga ttatttattc cccaggatga tgagagatcg aattctttta tcccggtata
    40561 catttttggc gagtcatgcg cgggtttgca accctgatta agtatttcca tgggcgcaga
    40621 aagcgcaaaa agaagatatt gttcttcctt attcatttgt taataatccc ttaagtgcat
    40681 taactgacta cttttttatt attgatgagt ataaacttga cgctttcaaa ataaaaagaa
    40741 aatcgaagca ttcacacatg aataaaaaat taatgtatat attcgcaatt tttatagttg
    40801 cagcaattac ctgtattagc caacccaaga aaacgacgtt gcgtgataaa gccatggtga
    40861 attatgcctt tgattattta agatcaccgg gcagtcttcc attcaccacg gcagccacgg
    40921 agctttccgc gattcatggt cactcaacgt cgcaatatcg ccttggagaa ttttatcttc
    40981 atggtagcga cggtaaacca ctggattata cacaggcgag atactggtat gagcaatcag
    41041 cggaacagga aaatccacgc gcgcaaagta aactggggtg gatctacctc aaaggtctgg
    41101 gggtcaaacc cgacacccgt aaagcaattc tctggtataa ggaagcagct gaacaagggt
    41161 atgctcatgc tcaatatact ttaggtttga tctacagaaa tggctcaggt attaatgtta
    41221 accattatga atctcaaaaa tggttaaaac tggccgccaa acaacattac aaaaatgcgg
    41281 aaagattact tgccgggctt cccgcacatt aacgtcgact gtcacccgaa cagatggagt
    41341 attttcccta tcaaaccagc aaagataaaa
//
LOCUS       DAFNBJ010000021        40493 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000021 DAFNBJ010000000
VERSION     DAFNBJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.021"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            290..376
                     /gene="leuL"
                     /locus_tag="L2X49_001137"
     CDS             290..376
                     /gene="leuL"
                     /locus_tag="L2X49_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBN4796251.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            469..2040
                     /gene="leuA"
                     /locus_tag="L2X49_001138"
     CDS             469..2040
                     /gene="leuA"
                     /locus_tag="L2X49_001138"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBN4796252.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2040..3131
                     /gene="leuB"
                     /locus_tag="L2X49_001139"
     CDS             2040..3131
                     /gene="leuB"
                     /locus_tag="L2X49_001139"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBN4796253.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3134..4534
                     /gene="leuC"
                     /locus_tag="L2X49_001140"
     CDS             3134..4534
                     /gene="leuC"
                     /locus_tag="L2X49_001140"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBN4796254.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4545..5150
                     /gene="leuD"
                     /locus_tag="L2X49_001141"
     CDS             4545..5150
                     /gene="leuD"
                     /locus_tag="L2X49_001141"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBN4796255.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5199..6377)
                     /gene="setA"
                     /locus_tag="L2X49_001142"
     CDS             complement(5199..6377)
                     /gene="setA"
                     /locus_tag="L2X49_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBN4796256.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6479..6610)
                     /gene="sgrT"
                     /locus_tag="L2X49_001143"
     CDS             complement(6479..6610)
                     /gene="sgrT"
                     /locus_tag="L2X49_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBN4796257.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6699..8354
                     /gene="sgrR"
                     /locus_tag="L2X49_001144"
     CDS             6699..8354
                     /gene="sgrR"
                     /locus_tag="L2X49_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBN4796258.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8388..8487
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8518..9501
                     /gene="thiB"
                     /locus_tag="L2X49_001145"
     CDS             8518..9501
                     /gene="thiB"
                     /locus_tag="L2X49_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBN4796259.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLIVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9477..11087
                     /gene="thiP"
                     /locus_tag="L2X49_001146"
     CDS             9477..11087
                     /gene="thiP"
                     /locus_tag="L2X49_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBN4796260.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11071..11769
                     /gene="thiQ"
                     /locus_tag="L2X49_001147"
     CDS             11071..11769
                     /gene="thiQ"
                     /locus_tag="L2X49_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBN4796261.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11883..12647)
                     /locus_tag="L2X49_001148"
     CDS             complement(11883..12647)
                     /locus_tag="L2X49_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBN4796262.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12733..13611)
                     /gene="araC"
                     /locus_tag="L2X49_001149"
     CDS             complement(12733..13611)
                     /gene="araC"
                     /locus_tag="L2X49_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBN4796263.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13950..15650
                     /gene="araB"
                     /locus_tag="L2X49_001150"
     CDS             13950..15650
                     /gene="araB"
                     /locus_tag="L2X49_001150"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBN4796264.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTGPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15661..17163
                     /gene="araA"
                     /locus_tag="L2X49_001151"
     CDS             15661..17163
                     /gene="araA"
                     /locus_tag="L2X49_001151"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBN4796265.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17363..18058
                     /gene="araD"
                     /locus_tag="L2X49_001152"
     CDS             17363..18058
                     /gene="araD"
                     /locus_tag="L2X49_001152"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBN4796266.1"
                     /translation="MLEDLKRLVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIATGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18133..20484
                     /gene="polB"
                     /locus_tag="L2X49_001153"
     CDS             18133..20484
                     /gene="polB"
                     /locus_tag="L2X49_001153"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBN4796267.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYIRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20649..23555
                     /gene="rapA"
                     /locus_tag="L2X49_001154"
     CDS             20649..23555
                     /gene="rapA"
                     /locus_tag="L2X49_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBN4796268.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23567..24226
                     /gene="rluA"
                     /locus_tag="L2X49_001155"
     CDS             23567..24226
                     /gene="rluA"
                     /locus_tag="L2X49_001155"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBN4796269.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24343..25158)
                     /gene="djlA"
                     /locus_tag="L2X49_001156"
     CDS             complement(24343..25158)
                     /gene="djlA"
                     /locus_tag="L2X49_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBN4796270.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25413..27767
                     /gene="lptD"
                     /locus_tag="L2X49_001157"
     CDS             25413..27767
                     /gene="lptD"
                     /locus_tag="L2X49_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBN4796271.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNSL"
     gene            27820..29106
                     /gene="surA"
                     /locus_tag="L2X49_001158"
     CDS             27820..29106
                     /gene="surA"
                     /locus_tag="L2X49_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBN4796272.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATADIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29106..30095
                     /gene="pdxA"
                     /locus_tag="L2X49_001159"
     CDS             29106..30095
                     /gene="pdxA"
                     /locus_tag="L2X49_001159"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBN4796273.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRTYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPLLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30092..30913
                     /gene="rsmA"
                     /locus_tag="L2X49_001160"
     CDS             30092..30913
                     /gene="rsmA"
                     /locus_tag="L2X49_001160"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBN4796274.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30916..31293
                     /gene="apaG"
                     /locus_tag="L2X49_001161"
     CDS             30916..31293
                     /gene="apaG"
                     /locus_tag="L2X49_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBN4796275.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31300..32142
                     /gene="apaH"
                     /locus_tag="L2X49_001162"
     CDS             31300..32142
                     /gene="apaH"
                     /locus_tag="L2X49_001162"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBN4796276.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32220..32699)
                     /gene="folA"
                     /locus_tag="L2X49_001163"
     CDS             complement(32220..32699)
                     /gene="folA"
                     /locus_tag="L2X49_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBN4796277.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32891..34753)
                     /gene="kefC"
                     /locus_tag="L2X49_001164"
     CDS             complement(32891..34753)
                     /gene="kefC"
                     /locus_tag="L2X49_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBN4796278.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34746..35276)
                     /gene="kefF"
                     /locus_tag="L2X49_001165"
     CDS             complement(34746..35276)
                     /gene="kefF"
                     /locus_tag="L2X49_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBN4796279.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35384..36715)
                     /locus_tag="L2X49_001166"
     CDS             complement(35384..36715)
                     /locus_tag="L2X49_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4796280.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36773..37060)
                     /gene="fixX"
                     /locus_tag="L2X49_001167"
     CDS             complement(36773..37060)
                     /gene="fixX"
                     /locus_tag="L2X49_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBN4796281.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37057..38343)
                     /locus_tag="L2X49_001168"
     CDS             complement(37057..38343)
                     /locus_tag="L2X49_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBN4796282.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38394..39335)
                     /locus_tag="L2X49_001169"
     CDS             complement(38394..39335)
                     /locus_tag="L2X49_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBN4796283.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39350..40120)
                     /locus_tag="L2X49_001170"
     CDS             complement(39350..40120)
                     /locus_tag="L2X49_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBN4796284.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9752 a        11133 c        10636 g         8972 t
ORIGIN      
        1 catataattc aatacaaatc atcaggatag gttttgcaac gcgtgcattt tgtccccttt
       61 ttcctcgttg attagatgca aaaatttatg ctgaaatatg tcaaccgatg aaaagcgtcg
      121 gtagttaagc agaaattaat atcgcttact ttaaccaccg cagcacaatt agctaatttt
      181 acgggtgcag aactcacgct ggcgggacgt ttttattgcg tcagggttga catccgtttt
      241 tgtatccagt aactctaaaa gcatatcgca ttcatctgga gctgatttaa tgactcacat
      301 cgttcgcttt atcggtctac tactactaaa cgcatcttct ttgcgcggta gacgagtgag
      361 cggcatccag cattaagcca gcacgcagtc aaacaaaaaa cccgcgccat tgcgcgggtt
      421 tttttatgcc cgaagcgagg cgctctaaaa gagacaagga cccaaaccat gagccagcaa
      481 gtcattattt tcgataccac attgcgcgac ggtgaacagg cgttacaggc aagcttgagt
      541 gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta tgggtgttga cgtgatggaa
      601 gtcggtttcc ccgtctcttc gccgggcgat tttgaatcgg tgcaaaccat cgcccgccag
      661 gttaaaaaca gccgcgtatg tgcgttagct cgctgcgtgg aaaaagatat cgacgtggcg
      721 gccgaatccc tgaaagtcgc cgaagccttc cgtattcata cctttattgc tacctcccca
      781 atgcacatcg ccaccaagct gcgcagcacc ctggacgagg tgatcgaacg cgctatctat
      841 atggtgaaac gcgcccgtaa ttacaccgat gatgttgaat tttcttgcga agatgccggg
      901 cgtacaccca ttgccgatct ggcgcgagtg gtcgaagcgg cgattaatgc cggtgccacc
      961 accatcaaca ttccggacac cgtgggctac accatgccgt ttgagttcgc cggaatcatc
     1021 agcggcctgt atgaacgcgt acctaacatc gacaaagcca ttatttccgt acatacccac
     1081 gacgatttag gcctggcggt cggcaactca ctggcggcgg tacatgccgg tgcacgccag
     1141 gtggaaggtg caatgaacgg gatcggcgag cgtgccggta actgttcgct ggaagaagtc
     1201 atcatggcga ttaaagttcg taaggatatt ctcaacgtcc acaccgccat taatcaccag
     1261 gagatatggc gcaccagcca gttagttagc cagatttgta atatgccgat cccggcaaac
     1321 aaagccattg ttggcagcgg cgcattcgct cactcctccg gtatccacca ggatggcgtg
     1381 ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat ctattggtct gaaccaaatc
     1441 cagctgaatc tgacctctcg ttcggggcgt gcggcggtga aacatcgcat ggatgagatg
     1501 gggtataaag aaagtgaata taatttagac aatttgtacg atgctttcct gaagctggcg
     1561 gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc tggccttcat cggtaagcag
     1621 caagaagagc cggagcattt ccgtctggat tacttcagcg tgcagtctgg ctctaacgat
     1681 atcgccaccg ccgccgtcaa actggcctgt ggcgaagaag tcaaagcaga agccgccaac
     1741 ggtaacggtc cggtcgatgc cgtctatcag gcaattaacc gcatcactga ctataacgtc
     1801 gaactggtga aatacagcct gaccgccaaa ggtcacggta aagatgcgct gggtcaggtg
     1861 gatattgtcg ccaactacaa cggtcgccgc ttccacggcg tcggcctggc caccgatatt
     1921 gtcgagtcct ccgccaaagc catggtgcac gtactgaaca atatctggcg tgccgcagaa
     1981 gtcgaaaaag agttgcaacg caaagctcaa cacaacgaaa acaacaagga aaccgtgtga
     2041 tgtcgaagaa ttaccatatt gccgtattgc cgggggacgg tattggtccg gaagtgatga
     2101 cccaggcgct gaaagtgctg gatgccgtgc gcaaccgctt tgcgatgcgc atcaccacca
     2161 gccattacga tgtaggcggc gcagccattg ataaccacgg gcaaccactg ccgcctgcga
     2221 cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg ctcggtaggc ggtccgaaat
     2281 gggaacattt accaccagac cagcaaccag aacgcggcgc gctgttgcct ttgcgtaagc
     2341 acttcaaatt attcagcaac ctgcgcccgg caaaactgta tcaggggctg gaagcattct
     2401 gtccgctgcg tgcagacatt gccgctaacg gcttcgacat cctgtgcgtg cgcgaactga
     2461 ccggcggcat ctatttcggt cagccaaaag gccgcgaagg tagcggacaa tatgaaaaag
     2521 cgtttgatac cgaggtgtat caccgttttg agatcgagcg tattgcccgc atcgcgtttg
     2581 aatctgctcg taaacgtcgc cacaaagtca cctcaatcga caaagctaac gtgctgcaat
     2641 cctctatttt atggcgtgaa atcgtcaacg agatcgccac ggaatacccg gatgtcgaac
     2701 tggcgcatat gtacatcgac aacgccacca tgcagctgat taaagatcca tcacagtttg
     2761 acgttctgct gtgctccaac ctgtttggcg acattctgtc tgacgagtgc gcaatgatca
     2821 ctggctcgat ggggatgttg ccttccgcca gcctgaacga gcaaggtttt ggtctgtatg
     2881 aaccggcagg cggctcagca ccagatatcg caggcaaaaa catcgccaac ccgattgcgc
     2941 aaattctatc gctggcactg ctgctgcgct acagcctgga tgccgatgat gcggcttgcg
     3001 ccattgaacg cgccattaac cgcgcattag aagaaggcat tcgcaccggg gatttagccc
     3061 gtggcgctgc cgccgttagt accgatgaaa tgggcgatat cattgcccgc tatgtggcag
     3121 aaggggtgta atcatggcta aaacgttata cgaaaaatta ttcgacgctc acgtagtgta
     3181 cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc cacctggtgc atgaagtgac
     3241 ctcaccgcag gcgttcgatg gtctgcgcgc ccacggtcgc ccggtacgtc agccgggcaa
     3301 aaccttcgcc actatggatc acaacgtctc tacccagacg aaagacatta atgcctgtgg
     3361 tgaaatggcg cgcatccaga tgcaggagct gattaaaaac tgcaaagaat ttggcgtcga
     3421 actgtatgac ctgaatcacc cgtatcaggg gatcgtccac gtaatggggc cggaacaggg
     3481 cgtcaccttg ccggggatga ccattgtctg cggcgactcg cataccgcca cccacggcgc
     3541 gtttggcgca ctggcctttg gtatcggcac ttccgaagtt gaacacgtac tggcaacgca
     3601 aaccctgaaa cagggccgcg caaaaaccat gaaaattgaa gtccagggca aagccgcgcc
     3661 gggcattacc gcaaaagata tcgtgctggc aattatcggt aaaaccggta gcgcaggcgg
     3721 caccgggcat gtggtggagt tttgcggcga agcaatccgt gatttaagca tggaaggtcg
     3781 tatgaccctg tgcaatatgg caatcgaaat gggcgcaaaa gccggtctgg ttgcaccgga
     3841 cgaaaccacc tttaactatg tcaaaggccg tttgcatgcg ccgaaaggca aagatttcga
     3901 cgacgccgtt gcctactgga aaaccctgca aaccgacgaa ggcgcaactt tcgataccgt
     3961 tgtcactctg caagcagaag aaatttcacc gcaggtcacc tggggcacca atccaggcca
     4021 ggtgatttcc gtgaacgaca atattcccga tccggcttcg tttgccgatc cggttgaacg
     4081 cgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa ccgggcattc cgctgaccga
     4141 agtggctatc gacaaagtgt ttatcggttc ctgtaccaac tcgcgcattg aagatttacg
     4201 cgcggcagcg gagatcgcca aagggcgaaa agtcgcgcca ggcgtgcagg cactggtggt
     4261 tcccggctct ggcccggtaa aagcccaggc ggaagcggaa ggtctggata aaatctttat
     4321 tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg tgtctggcga tgaacaacga
     4381 ccgtctgaat ccgggcgaac gttgtgcatc caccagcaac cgtaactttg aaggccgcca
     4441 ggggcgcggc gggcgcacgc atctggtcag cccggcaatg gctgccgctg ctgctgtgac
     4501 cggacatttc gccgacattc gcaacattaa ataaggagca caccatggca gagaaattta
     4561 tcaaacacac aggcctggtg gttccgctgg atgccgccaa tgtcgatacc gatgcaatca
     4621 tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt tggcgcgcat ctgtttaacg
     4681 actggcgttt tctcgatgaa aaaggccaac agccaaaccc ggacttcgtg ctgaacttcc
     4741 cgcagtatca gggcgcttcc attttgctgg cacgagaaaa cttcggctgt ggctcctcgc
     4801 gtgagcacgc accgtgggca ttgaccgact acggttttaa agtggtgatt gcgccgagtt
     4861 ttgctgacat cttctacggc aatagcttta acaaccagct gctgccggtg aaattaagcg
     4921 atgcagaagt ggacgaactg tttgcgctgg tgaaagccaa tccggggatc catttcgacg
     4981 tggatctgga agcgcaagag gtgaaagcgg gagagaaaac ctatcgcttt accatcgatg
     5041 ccttccgccg ccactgcatg atgaacggtc tggacagtat tgggcttacc ttgcagcacg
     5101 acgacgccat tgccgcttat gaagcaaaac aacctgcgtt tatgaattaa tccccttgcc
     5161 cggtcaaatg accgggcttt ccgctatcgt ccacgtcatc aaacgtcttt aacctttgcg
     5221 gttaaaaata atgcgacaac agaaataacc gcaattaccc agtagacagc aaagtgcccc
     5281 caactttgtg caattgctcc ctgaataacg ccagccagaa ttaccccggt agaaatactg
     5341 ttagtaaata aggtggtagc tgcccccgct cttccaggca ttaaatcctg aaaccatagc
     5401 atcccaatac ccgcaacaat gccgataaat acagcgttaa aaagttgcag cgtcatcaac
     5461 gccatacggc tatgaaagaa aatcaatccg gtgtaaaaca gtactcctgc cgccactgct
     5521 atgaccatca ttcgccgctt accataacgt ttgacatagt agccagccag aatcattgct
     5581 ggtatttcca gtccagctgc cgtccccatc aggaaacccg ccagtttgtc tggcaatcct
     5641 aactcgctac tgatccacaa cggcatatca ataatgtaca tggtgttgca ggtccacatt
     5701 aacgtcgagg cgacaaataa catccgtacg ttactatcct gccagccacc ttgcattgat
     5761 aaagcatttt ccgacggcag ttctacccgc gccacagacg gaagcataaa tgcaatcaat
     5821 accagactga gtgtgaatat cccggcggca atcgaaaaca tcaccgtaaa gccgtaattc
     5881 aacgccagca taaaggccaa cggtggaccg ataacccatg ccagagaaag ctgcgcacgc
     5941 atcaccgagc taaacatcac cacttctcgc gccgagttat ccgcatattc ccgcgccaga
     6001 gcaaataact gtggcattgc cgtattggcc agagatgcca gaagcacacc acaggtgata
     6061 agcgtcagat aatgacgatt aaatgcaaac aatagcgcat tgccgatagc catcaaacag
     6121 caaaatataa tcagttttcg ccgatcgccc tgactgtcag aacgttttgc caaccagagg
     6181 cttacgccga tcccagcaat agcattcacc gtataaaaga gtccgatcca gaaaggttgc
     6241 gcgccaacct cacgactcag aaataagctc aatgtaggag cctgtagcgc cccggccacc
     6301 cccatcataa aagcgaccag cataaatgcc gcgtaaacac cgttcatacg gcgagccatc
     6361 gtcattatcc agatcatacg ttcccttttt agcgcggcga gaataaaaaa aaccagcagg
     6421 tataatctgc tggcgggtga ttttacacca atactcagtc acacatgatg caggcaagtc
     6481 aactttcaga attgcggtca tcccactgca tcagtccttc aagcatggtt aatcgttgtg
     6541 ggacgcttaa ccaacgcaac cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa
     6601 actgacgcat ggggcacctc cttgcttcat cgttaggaat tattgactta atatagggaa
     6661 aataaaattg ctgtcttttg cgcaggagtt ccccttttat gccatctgct cgtctgcaac
     6721 aacagttcat ccgcctgtgg caatgctgcg agggtaaatc gcaggacaca acgctcaacg
     6781 aactggcagc gttattgagc tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc
     6841 aggatcgcgg ctggctgacg tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga
     6901 cattcctcta taccgggctg gcgcttcagc aacagcgggc ggaagacctg ctggagcagg
     6961 atcgtatcga ccaactagtg cagttggttg gcgacaaagc gactgtgcgg caaatgctgg
     7021 tttctcatct gggccgcagc ttccgccagg ggcggcacat cctgcgcgtg ctctactatc
     7081 gtccgttgcg taatctgcta cctggcagcg cattgcgccg ttccgaaacc catattgccc
     7141 ggcaaatctt cagttcgcta acgcgcataa atgaggaaaa tggggaactg gaagcagaca
     7201 tcgcccacca ctggcagcaa atatcaccgc ttcactggcg tttctttttg cgtccaggag
     7261 tccattttca tcatggtcgt gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa
     7321 tcaatacgct gccgctctat tcgcatattg cggacattgt gtcgccgacg ccctggacgc
     7381 tggatatcca tctcacgcaa ccggaccgct ggttaccgtt actgctggga caagttccgg
     7441 cgatgatcct gccgcgcgaa tgggaaaccc tcagtaactt tgccagccat cccatcggca
     7501 ccggtccgta tgcggtgatc cgcaacacca ccaatcaact gaaaattcag gcattcgatg
     7561 acttcttcgg ttaccgggca ttaatcgatg aagttaacgt ctgggttctg ccggaaattg
     7621 ccgacgagcc agctggaggt ctgatgctaa aaggtccaca gggcgaggaa aaagagattg
     7681 aaagccgcct ggaggaaggt tgctactatt tactgttcga cagccgcacc catcgcgggg
     7741 cgaatcagca agtcagggac tgggtaagct atgtgctttc tccaactaat ctggtctatt
     7801 tcgctgagga acagtaccag caactgtggt tcccggctta tggactgctc ccccgttggc
     7861 accatgcccg caccataaag agcgaaaaac cggctggcct ggaaagcctc accctgacct
     7921 tttatcagga tcacagtgag catcgggtga ttgccgggat catgcagcag attctggcaa
     7981 gtcaccaggt cacgctggaa atcaaagaga tcagctacga tcagtggcat gaaggagaga
     8041 tcgagagcga tatctggctt aatagcgcca actttacgct gccgctggat ttttcgctgt
     8101 tcgcgcactt gtgcgaggtg ccgctgctcc aacactgtat tccgatcgac tggcaagccg
     8161 acgccgcccg ctggcgcaat ggcgaaatga acctggcgaa ctggtgccag caactggtcg
     8221 ccagtaaagc aatggtgccg cttatccacc actggctgat cattcaggga caacgcagta
     8281 tgcgcggcct gcgtatgaat accctcggct ggttcgattt taaatcagcg tggtttgcgc
     8341 cgccggatcc atgattgctg gtagataaca aaatcactac actaacgccg ttctcaacgg
     8401 ggtgccacgc aaacgcgtgc gctgagaaaa tacccgtcga acctgatccg gataacgccg
     8461 gcgaagggat ttgaggctcc ttctcaagtc ctttgccact cttttttgag gtgcaaagtg
     8521 ttaaaaaaat gtcttcccct gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt
     8581 ctgattgttt atacctacga ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa
     8641 aaagcttttg aagccgactg taactgcgaa ctgaaactgg tggcgctgga agatggcgtt
     8701 tcgcttctca accgtctacg gatggaaggc aaaaatagta aagccgatgt ggtgctgggg
     8761 ctggataaca atctgttaga cgccgccagt aaaaccggac tatttgccaa aagcggtgtg
     8821 gcagcggatg ccgttaacgt tcccggcggc tggaataatg acactttcgt cccgtttgat
     8881 tacggctatt tcgccttcgt ctatgacaag aacaaactga aaaacccgcc gcaaagcctg
     8941 aaagaattgg ttgagagcga tcaaaactgg cgggtgattt atcaggatcc gcgcaccagt
     9001 acgccggggc tgggcctgct gttgtggatg caaaaagtct atggcgatga cgccccacaa
     9061 gcctggcaaa aactggcgaa gaaaaccgtc acggtcacca aaggctggag cgaagcctac
     9121 ggcctgtttt taaaaggtga aagcgatctg gtactgagtt acaccacctc tccggcttat
     9181 cacattctcg aagagaaaaa agataactac gccgccgcga actttagcga aggtcactat
     9241 ctgcaggtgg aagtcgccgc ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa
     9301 ttcctccagt ttatggtttc tccggctttt cagaatgcga tcccaaccgg taactggatg
     9361 tatccggtgg caaatgtcac gctgcctgcc gggtttgaac aattgaccaa accggccacc
     9421 acgctggagt tcacaccagc tgaagtggcg gcacaacgtc aggcatggat tagcgaatgg
     9481 caacgcgccg tcagccgtta attcccggct ggttaattcc aggtgtaagc gccgccacgc
     9541 tggtggtagc ggttgcgctc gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta
     9601 actgggtggc agtctggcag gacagctacc tgtggcatgt ggtgcgcttc tccttctggc
     9661 aggcgtttct ctcggcgcta ctctctgtcg tacccgcgat attcctcgcc cgcgccctct
     9721 atcgcaggcg ctttccgggt cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc
     9781 tcccggtgct agttgctgtt ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg
     9841 catcgctctg ccaatcgctc ggtctggagt ggaccttttc gccctacggc ctgcaaggta
     9901 ttttgctggc gcacgtattt tttaatctgc cgatggcgag ccgcttatta ctccaggcac
     9961 tggaaaacat tcccggcgaa caacgtcagc ttgccgccca gcttgggatg cgcggctggc
    10021 attttttccg cttcgtcgaa tggccgtggt tacggcgaca aatcccgccg gttgctgcac
    10081 ttatctttat gctctgtttc gccagcttcg ccaccgtgct atcactgggc ggcggtccgc
    10141 aggcgaccac tatcgagctg gctatttatc aggcgctgag ttacgactac gatcctgccc
    10201 gcgcggcgat gctggcgctg atccagatgg tgtgttgcct tgggctggta ctgttgagtc
    10261 agcgattgag taaggccatt gcgccaggca ccacgctgct gcaaggctgg cgcgacccgg
    10321 acgatcgtct gcatagccgc atttgcgaca cggtgttaat tgtgctggcg ctgctgctgt
    10381 tgctgccacc gttactggcg gtgatcgtcg atggcgtaaa tcgccagttg ccggaagtgc
    10441 tggcacaacc ggtgctgtgg caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag
    10501 gtgtattgtg cgtagtgctg accatgatgc tgctatggag cagtcgcgaa cttcgggcgc
    10561 ggcagaaaat gctggcgggt caggcgctgg agatgagcgg catgttgatc ctcgccatgc
    10621 cggggattgt gctggctacc ggcttctttt tactgctcaa caacaccatt ggcctgccgc
    10681 aatctgctga cggcattgtg attttcacca atgcgttaat ggcgatccct tatgcgctga
    10741 aagtactgga aaacccgatg cgcgatatca ccgcccgcta cagtatgttg tgtcagtcgc
    10801 tgggcattga aggctggtcg cgcttaaaag tggtcgagct gcgcgccctg aaacgtccac
    10861 tggcgcaggc gctggctttt gcctgcgtgc tgtcgattgg tgattttggc gtagtggcgt
    10921 tgttcggtaa cgatgatttc cgtaccctgc cgttttatct ctaccagcaa attggctcct
    10981 atcgcagcca ggacggtgcg gtcaccgcgt taattctgct actactctgt tttctgctgt
    11041 ttaccgtgat tgaaaaacta ccggggcgaa atgttaaaac tgactgatat cacctggctt
    11101 tatcaccatt tgccgatgcg ttttagctta acggtagaac gcggcgagca ggtggcaatc
    11161 ctcgggccaa gcggcgcggg taaaagtacc ctgctgaatt tgatcgccgg ttttctgacg
    11221 ccagccagcg gttcgctgac tatcgacggc gttgatcaca ccacgacgcc gccgtcacgc
    11281 cgtccggtat cgatgctgtt tcaggagaac aacctgttca gccacctgac ggtcgcgcag
    11341 aacatcgggc tggggcttaa tccgggattg aagctgaacg cggcgcagca ggagaaaatg
    11401 cacgctatcg cccgccagat ggggattgat aatttaatgg cgcggttacc gggcgagctt
    11461 tccggcggtc agcgacagcg cgtggcgtta gcgcgttgtc tggtgcgcga acagccgatt
    11521 ttattgctcg atgaaccgtt ctctgcgctc gatccggcgt tacgtcagga gatgctgacg
    11581 ctggtgagca cgagctgcca gcagcaaaaa atgacgctat tgatggtgtc gcacagtgtg
    11641 gaagatgcgg cgcggatcgc cacacgctcg gtggtggtcg ccgacgggcg catcgcctgg
    11701 cagggtaaga ccaatgagtt gttgagcggt aaggcgagtg cttcggcact attggggatt
    11761 acgggttagt atgcactgtc ggatgcggcg tgaacgcctt atccgaccta cgggtagtgc
    11821 gttttgtagg cctgataaga cgcggtcagc gtcgcatcag gcaacccgca caagactatc
    11881 tcctaacccc caaccacttt acgcaaaata tcgatataca ccggcatcaa cgggtggcga
    11941 attaacacca ccagcgccac cacgccgatg gcagaaatta acggcgtcag ccacaacaaa
    12001 cggccgcgcg acaaataatg actcaaacgg tcagtcgctt taccactgcg ccataaccgc
    12061 cagcacagcc agccaccaac ccacaaaaac accgctgttg ccagcagcaa ccatttaaac
    12121 tcaccgctct gcattccggc tggaatatcg atcgccgcgc ccgccagaat ccctggcagg
    12181 aagtaaaacg gtggccacag caggcagccg ataatattcg gcgtaatgaa ttttgccacc
    12241 ggcagatcca gcattcccgc caccattggc accagcggac gcgtcgggcc aacaaaacga
    12301 ccgaccagaa tggtgaacat gctgtgttga tgcaacgcat gttcagtttt atcgagtaat
    12361 gctttgtttt tcttcagaaa tgaccagcga tgcaacggct ttttaaaacg ccagcccagc
    12421 cagaaggaga tccagtcgcc cagcaggcaa ccgacgatcc ctgccagcca ggcgtgccag
    12481 aaacttaact cgccgctgcc aatcagcgct cccagccccg ccatcagcac cgtaccgggt
    12541 agaatcaaac cgaccagcgc cagcgactcc agaaaggcca ccaacaccac cgccatcaat
    12601 gaatacacgg tagattgggt aataaagtgt tccagcaatg cttgcataat gtgcctgtca
    12661 aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg
    12721 attcgttacc aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc
    12781 acggaactcg ctcgggctgg ccccggtgca ttttttaaat actcgcgaga aatagagttg
    12841 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag
    12901 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg
    12961 ctggcggaaa agatgtgaca gacgcgacgg tgacaagcaa acatgctgtg cgacgctggc
    13021 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg
    13081 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
    13141 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
    13201 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaacc
    13261 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
    13321 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc
    13381 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt
    13441 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
    13501 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg
    13561 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat
    13621 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg
    13681 cgtcttttac tggctcttct cgctaaccca accggtaacc ccgcttatta aaagcattct
    13741 gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg
    13801 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt
    13861 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc
    13921 atacccgttt ttttggatgg agtgaaacga tggcgattgc aattggcctc gattttggca
    13981 gtgattctgt gcgagctttg gcggtggact gcgccagcgg tgaagagatc gccaccagcg
    14041 tagagtggta tccccgttgg caaaaagggc aattttgtga tgccccgaat aaccagttcc
    14101 gtcatcatcc gcgtgactac attgagtcaa tggaagcggc actgaaaacc gtgcttgcag
    14161 agcttagcgt cgaacagcgc gcagctgtgg tcgggattgg cgttgacaca accggctcga
    14221 cgcccgcacc gattgatgcc gacggtaacg tgctggcgct gcgcccggag tttgccgaaa
    14281 acccgaacgc gatgttcgta ttgtggaaag accacactgc ggttgaagaa gcggaagaga
    14341 ttacccgttt gtgccacgcg ccgggcaacg ttgactactc ccgctatatt ggcggtattt
    14401 attccagcga atggttctgg gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg
    14461 cgcaatctgc cgcatcgtgg attgagctgt gcgactgggt gccagctctg ctttccggta
    14521 ccaccggccc gcaggatatt cgtcgcggac gttgcagcgc cgggcataaa tctctgtggc
    14581 acgaaagctg gggcggcctg ccgccagcca gtttctttga tgagctggac ccgatcctca
    14641 atcgccattt gccttccccg ctgttcactg acacctggac tgccgatatt ccggtgggca
    14701 ccttatgccc ggaatgggcg cagcgtctcg gcctgcctga aagcgtggtg atttccggcg
    14761 gcgcgtttga ctgccatatg ggcgcagttg gcgcaggcgc acagcctaac gcactggtaa
    14821 aagttatcgg tacttccacc tgcgacattc tgattgccga caaacagagc gttggcgagc
    14881 gggcagtgaa aggtatttgc ggtcaggttg atggcagcgt ggtgcctgga tttatcggtc
    14941 tggaagcagg ccaatcggcg tttggtgata tctacgcctg gtttggtcgc gtactcggct
    15001 ggccgctgga acagcttgcc gcccagcatc cggaactgaa agcgcaaatc aacgccagcc
    15061 agaaacaact gcttccggcg ctgaccgaag catgggccaa aaatccgtct ctggatcacc
    15121 tgccggtggt gctcgactgg tttaacggcc gccgcacacc gaacgctaac caacgcctga
    15181 aaggggtgat taccgatctt aacctcgcta ccgacgctcc gctgctgttc ggcggtttga
    15241 ttgctgccac cgcctttggc gcacgcgcaa tcatggagtg ctttaccgat caggggatcg
    15301 ccgtcaataa cgtgatggcg ctgggcggca tcgcgcggaa aaaccaggtc attatgcagg
    15361 cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc ctctgaccag tgctgtgcgc
    15421 tcggtgcggc gatttttgct gccgtcgccg cgaaagtgca cgcagacatc ccctcagccc
    15481 agcaaaaaat ggccagtgcg gtagagaaaa ccctgcaacc gtgcagcgaa caggcacaac
    15541 gctttgaaca gctttatcgc cgctatcagc aatgggcgat gagcgccgaa caacactatc
    15601 ttccaacttc cgccccggca caggctgccc aggccgttcc gactctataa ggacacgata
    15661 atgacgattt ttgataatta tgaagtgtgg tttgtcattg gcagccagca tctgtatggc
    15721 ccggaaaccc tgcgtcaggt cacccaacat gccgagcacg tcgttaatgc gctgaatacg
    15781 gaagcgaaac tgccctgcaa actggtgttg aaaccgctgg gcaccacgcc ggatgaaatc
    15841 accgctattt gccgcgacgc gaattacgac gatcgttgcg ctggtctggt ggtgtggctg
    15901 cacaccttct ccccggccaa aatgtggatc aacggcctga ccatgctcaa caaaccgttg
    15961 ctgcaattcc acacccagtt taacgcggcg ctgccgtggg acagtatcga tatggacttt
    16021 atgaacctga accagactgc tcatggcggt cgcgagttcg gcttcattgg cgcgcgtatg
    16081 cgtcagcaac atgccgtcgt taccggtcac tggcaggata aacaagccca tgagcgtatc
    16141 ggctcctgga tgcgtcaggc ggtctctaaa caggataccc gtcatctgaa agtctgccga
    16201 tttggcgata acatgcgtga agtggcggtc accgatggtg ataaagttgc cgcacagatc
    16261 aagttcggtt tctccgtcaa tacctgggcg gttggcgatc tggtgcaggt ggtgaactcc
    16321 atcagcgacg gcgatgttaa cgcgctggtc gatgagtacg aaagctgcta caccatgacg
    16381 cctgcaacac aaatccacgg cgaaaaacga cagaacgtgc tggaagcggc gcgtattgag
    16441 ctggggatga agcgtttcct ggaacaaggt ggcttccacg cgttcaccac cacctttgaa
    16501 gatttgcacg gtctgaaaca gcttccaggt ctggccgtac agcgtctgat gcagcagggt
    16561 tacggctttg cgggcgaagg cgactggaaa actgccgccc tgcttcgcat catgaaggtg
    16621 atgtcaaccg gtctgcaggg cggtacctcc tttatggagg actacaccta tcacttcgag
    16681 aaaggcaatg acctggtgct cggctcccat atgctggaag tctgcccgtc gattgcagta
    16741 gaagagaaac cgatcctcga cgttcagcat ctcggtattg gtggtaagga cgatcctgcc
    16801 cgcctgatct tcaataccca aaccggtcca gcgattgttg ccagcctgat tgatctcggc
    16861 gatcgttacc gtctgctggt taactgtatc gacacggtga aaaccccgca ctccctgccg
    16921 aaactgccgg tggcgaatgc gctgtggaaa gcgcaaccgg atctgccaac tgcttccgaa
    16981 gcgtggatcc tcgctggtgg cgcgcaccat accgtcttca gccatgcgct gaacctcaac
    17041 gatatgcgcc agttcgccga gatgcacgac attgaaatca cggtgattga taacgacacc
    17101 cgcctgccag cgtttaaaga cgcactgcgc tggaacgaag tgtattacgg atttcgtcgc
    17161 taagtagcca catcaggtat gtaacgcctg atgcgacgct gacgcgtctt atctggccta
    17221 cgcgctgtgt ttttgtaggc cggataagca aagcgcatcc ggcattcaac gcctgatgcg
    17281 acgctggcgc gtcttatcag gcctacgcgc tgcgattttg tagaccggat aagcaaagtg
    17341 catccggcac gaaggagtca acatgttaga agatctcaaa cgcctggtat tagaagccaa
    17401 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    17461 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    17521 tgacgatatg gtcgtggtta gcatcgcaac cggtgaagtg gttgaaggta cgaaaaagcc
    17581 ctcctctgac acgccaactc accggctgct ctatcaggca ttcccgtcca ttggcggcat
    17641 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt cgattccagc
    17701 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    17761 cgacgcagaa atcaacggtg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    17821 cgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtc ctggtccatt ctcacggccc
    17881 atttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    17941 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    18001 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    18061 actgtataaa accacagcca atcaaacgaa accaggctat aatcaagcct ggttttttat
    18121 tggattttca acgtggcgca ggcaggtttt atcttaaccc gacactggcg ggataccccg
    18181 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    18241 gcaccgcaag agtccgtggc gtttattcct gccgatcagg ttccccgcgc tcagcatatt
    18301 ttgcagggtg aacaaggctt tcgcctgacg ccgctggcgt taaaggattt tcaccgccag
    18361 ccggtgtatg gcctttactg tcgcgcccat cgccagttga tgaattacga aaagcgcctg
    18421 cgtgaaggtg gcgttaccgt ttacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    18481 gagcgtttta tcacctcgcc agtgtgggtc gagggtgata tacgcaatgg cgctatcgtt
    18541 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctattgat
    18601 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggttg cgggcagcgc
    18661 atcgtttata tgctggggcc agagaatggc gacgcctccg cgctcgattt tgaactggaa
    18721 tacgtcgcca gtcgcccgca gctactggaa aaactcaacg cctggtttgc caactacgat
    18781 cctgatgtga tcatcggttg gaacgtggtg cagtttgatc tgcgaatgct gcaaaaacat
    18841 gccgagcgtt atcgtattcc gctgcggctg gggcgcgata acagtgagct ggagtggcgc
    18901 gagcacggct ttaaaaacgg tgtgtttttt gctcaggcta aaggtcggct aattatcgac
    18961 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    19021 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    19081 attgaccgcc gtttcgccga agataaacct gcgctggcga cttataacct gaaagattgc
    19141 gagctggtga cgcagatttt ccataaaact gaaatcatgc cgtttttgct cgaacgagca
    19201 acggtgaacg gcctgccggt agaccgccac gggggatcgg tggcggcgtt tggtcatctc
    19261 tattttcccc gtatgcaccg cgctggttat gtcgcgccta atctcggcga agtgccgcca
    19321 cacgccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    19381 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    19441 gggctggtgg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    19501 gcctggttct cacgagaaaa acattgcctg ccggagattg tgactaacat ctggcacggg
    19561 cgcgatgaag ccaaacgcca gggtaacaaa ccgctgtcgc aggcgctgaa aatcatcatg
    19621 aatgcctttt atggcgtgct cggcaccacc gcctgccgct tcttcgatcc gcggctggca
    19681 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcgtt gattgaagca
    19741 cagggctacg acgttatcta cggcgatacc gactcaacgt ttgtctggct gaaaggcgca
    19801 cattcggaag aagaagcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    19861 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaacc
    19921 catttctgcc gttttctgat gccaaccatt cgcggagccg ataccggcag taaaaagcgt
    19981 tatgccggac tgattcagga gggcgacaag cagcggatgg tgtttaaagg gctggaaacc
    20041 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgtatc
    20101 ttccgtaacg agccatatca ggaatacatc cgcgaaacca tcgacaaact gatggcgggt
    20161 gaactggatg cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag cgaatatcag
    20221 cgtaatgttc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgc
    20281 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    20341 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    20401 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    20461 accgggcaac ttgggctatt ttgagcaaaa aaaagagttc gccagatacc attttgatgc
    20521 gtgacgaatg ctttgccatc cagtaccata gcgccctttc cattcctgga cctgaataac
    20581 gccactacct cataagcacg gtagcgggtg gttattgcct gcaattaaag atatagagcc
    20641 gaacacatat gccttttaca cttggtcaac gctggatcag cgatacagaa agcgaattgg
    20701 gacttggaac cgttgtcgcg gtggatgcgc gaactgtcac tttacttttc ccatctactg
    20761 gtgaaaaccg tctgtacgca cgcagtgatt cccccgtgac ccgcgtgatg ttcaaccctg
    20821 gtgataccat taccagccat gacggctggc agatgcaagt cgaagaagta aaagaagaaa
    20881 atggcttgct gacctatatc ggtactcgcc tggatactga agagtccggc gtagccctgc
    20941 gtgaagtttt ccttgatagc aaactggtgt tcagcaaacc gcaggaccgt ctgtttgccg
    21001 ggcagattga ccgtatggac cgctttgcgc tgcgttatcg cgcgcgtaag tattccagcg
    21061 aacagttccg tatgccgtac agcggcctgc gcggtcagcg taccagcctg attccgcatc
    21121 agctcaacat cgctcatgat gttggtcgcc gccacgcgcc gcgcgtcctg ctggctgacg
    21181 aagtgggttt agggaaaacc attgaagccg ggatgatcct gcatcagcaa ctgctctctg
    21241 gcgctgctga acgtgtgctg attatcgtcc cggaaacctt acagcatcag tggctggtag
    21301 aaatgctgcg ccgtttcaac ctgcgctttg cgctatttga tgatgagcgt tatgccgaag
    21361 ctcagcacga tgcttacaac ccgtttgaca ccgaacagct ggtgatttgc tcgctggatt
    21421 ttgcccgtcg tagcaaacag cgcctggaac atctctgtga agccgaatgg gacctgctgg
    21481 tggtcgatga agcgcatcac ctggtgtgga gcgaagatgc gccaagccgt gaatatcagg
    21541 ccattgaaca actggcagag cacgtgccgg gcgttctgct gctgaccgcg accccggaac
    21601 agctggggat ggaaagccac ttcgcccgtc tgcgtctgct ggacccgaac cgtttccacg
    21661 attttgcgca gttcgttgaa gagcagaaaa attatcgtcc ggttgcggac gccgttgcca
    21721 tgctgctggc aggtaacaaa ctgagcaatg acgaactgaa catgctcggc gagatgatcg
    21781 gcgagcagga tatcgagccg ctgttgcagg cagcaaacag cgacagcgaa gatgcccaga
    21841 gcgcccgtca ggagctggtt tcgatgctga tggatcgcca cggcaccagc cgcgtgctgt
    21901 tccgtaacac gcgtaacggt gtgaaaggat tcccgaaacg cgagctgcac accattaaac
    21961 tgccgctacc gacgcagtat cagacggcta ttaaagtctc cggcattatg ggcgcacgta
    22021 aaagtgcgga agatcgtgct cgcgatatgc tctacccgga gcgtatttat caggaatttg
    22081 aaggtgataa cgccacctgg tggaacttcg atccgcgcgt tgagtggctg atgggctacc
    22141 tgaccagcca tcgctctcag aaagtgctgg tgatctgcgc caaagctgcc actgcgctgc
    22201 aactggagca ggtactgcgc gaacgtgaag gtattcgcgc tgcggtgttc cacgaaggta
    22261 tgtcgattat cgaacgtgac cgcgctgccg cctggtttgc cgaagaagac accggcgcac
    22321 aggtactgct gtgctcagaa atcggttctg aaggacgtaa cttccagttc gccagccaca
    22381 tggtgatgtt tgacctgcca ttcaacccgg atctactgga gcagcgtatt ggtcgtctgg
    22441 atcgtatcgg ccaggcgcac gatattcaga tccatgtgcc ttatctggag aaaaccgctc
    22501 agtcggtgct ggtgcgctgg tatcacgaag gtctggatgc atttgagcac acctgcccga
    22561 ccggacgcac tatttacgat agcgtataca acgatctgat taactatctg gcttcaccgg
    22621 atcaaaccga aggctttgac gatctgatca aaaactgccg cgagcaacat gaagcgctga
    22681 aagcacagct ggaacagggt cgtgaccgcc tgctggaaat ccactccaac ggtggcgaaa
    22741 aagcccaggc actggcagaa agcattgaag agcaggatga cgataccaac ctgatcgcct
    22801 tcgccatgaa cctgttcgat attatcggta tcaatcagga cgatcgcggc gacaacatga
    22861 tcgtgctgac gccgtccgat catatgctgg tgccggactt ccctggcctg tcggaagatg
    22921 gcatcaccat cacctttgat cgtgaagtgg cgctggcgcg tgaagatgca cagtttatta
    22981 cctgggagca tccgctgatc cgcaacggtc tggatctgat tctttctggc gataccggta
    23041 gcagcacgat ttcactgtta aaaaacaaag cgttgccggt aggtacgctg ttggtggaac
    23101 tgatttatgt ggttgaagcc caggctccga agcagttgca gctcaaccgc ttcctgccac
    23161 cgacgccggt acgtatgctg ctggataaaa acggcaacaa cctggcggcg caggtagagt
    23221 ttgaaacctt taaccgccag cttaacgcgg ttaaccgtca caccggcagc aaactggtta
    23281 acgccgtgca gcaggatgtt cacgctatcc ttcaactggg tgaagcgcag atcgagaaat
    23341 ctgcccgtgc attgattgat gcagcgcgta acgaagccga cgaaaaactg tctgccgagc
    23401 tgtctcgtct ggaagctctg cgtgcagtga acccgaacat tcgtgacgac gaactgaccg
    23461 ccattgagag caaccgtcag caggtaatgg aaagcctgga tcaggcaggt tggcgtctgg
    23521 atgccctgcg tttgatcgtt gtaacgcatc agtaacggag ccgaaaatgg ggatggaaaa
    23581 ctacaatcca ccgcaggaac cctggttggt tatcctgtat caggatgacc atattatggt
    23641 ggtcaacaag ccgagcggtt tgttgtcagt gccgggtcgt ctggaagagc acaaagacag
    23701 cgtgatgacg cgcattcagc gtgattatcc gcaggcagaa tcggtgcatc gtctggatat
    23761 ggctaccagc ggcgtgattg tagtggcgct gaccaaagcc gcggagcggg agttaaaacg
    23821 ccagttccgc gagcgcgagc cgaaaaagca gtatgtggcc cgcgtctggg ggcatccatc
    23881 ccccgcagaa ggtctggtgg atctgccgct gatttgcgac tggccaaacc gcccgaaaca
    23941 gaaagtctgt tacgaaacgg gtaaacctgc gcagacggaa tatgaagtgg tggagtatgc
    24001 ggcggataac acggcaagag tggtgttaaa accgattacc gggcgttcgc atcaattgcg
    24061 tgtgcatatg ctggcgctgg ggcatccgat tctcggcgat cgtttttatg catcaccaga
    24121 agcgagagcg atggcaccgc gtttgttgct acatgcagag atgttgacga ttacccatcc
    24181 ggcgtatggc aatagtatga cgtttaaagc gccagcggat ttttaatcgt cacgctggcg
    24241 cagttcggta ggcggataag gcgttcacgc cgcatccggc aattgtgcgc cgattgcctg
    24301 atgcgacgcg ggccgcgtct tatcaggcct acagtttaaa acttatttaa accctttctg
    24361 ctgctttatc agctcatatg cctgctgaat ttcctgcgct ttctgcttcg ccatctccat
    24421 catctcaggc ggcaaacctt tcgccaccag cttatcggga tggtgttcac tcatcagctt
    24481 acggtaggca cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca gcacattaca
    24541 ggcatcttcc agcgttgggc cacgctgcgc ttgctgccag ttaccaccgc cagtttgctg
    24601 ctgataaccg ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg
    24661 agcgcgggag atccctaatt cttctgcaat gacatacagc accgcccgtt cattcgggtg
    24721 cagtgaacca tcagcaaacg ccgcctgaat ctggatctcc agaaacatac gaattaagtc
    24781 aaaacgacca aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattgtc
    24841 tgattttccc acccggaacg cattttgcgc cgcagtacgg gaagcgccat gaagattcat
    24901 tcggtccatc aactggctgg cgatatgaat atcagcctcc gtgacgcgac ctttggattt
    24961 ggttaaatgc cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg
    25021 gttggcgaac cacgccattt tacggctacg ggctttatca aacatatggc caattaacag
    25081 gcctaacact acgccccaaa agccgccgcc catcagtaag gccacggcca cgccaatgat
    25141 ttttccccaa tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca
    25201 caatataaag gtgcttttac cgttttccgg ctgcggtcaa ttgtgggaca tcgcctataa
    25261 tttgcattat catacctgtc attcaatgcc gtgcctaaca ccactgacgc tattcggaca
    25321 ggattaacac tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa
    25381 cgttaccgat gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg
    25441 ccaccatgat tgccaccgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac
    25501 agtgcatgtt gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact
    25561 tacccgtgac tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta
    25621 ctggcagcgt ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc
    25681 atcaaaaaga ggcaccagga caaccggagc cggtacgtac cgttgatgcg ctcggtaatg
    25741 tccattacga cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca
    25801 ccaaagatac caacgtctgg gaaggtgatt accagatggt gggtcgccag ggtcgcggta
    25861 aagcggacct gatgaaacaa cgtggcgaaa accgctatac cattctggat aacggtagct
    25921 ttacctcctg tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg
    25981 accgcgaaga acaagttgcg gagatctgga acgcccgctt taaggtgggt ccggtaccga
    26041 tcttttatag cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga
    26101 tcccgaacgc caagtacacc accaccaact actttgagtt ctacctgcca tattactgga
    26161 acatcgcgcc aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaaca
    26221 tcatgtggga gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg
    26281 actatctgcc ttcagataaa gtctatgaag atgaacaccc gaacgatgac agttcacgtc
    26341 gttggttatt ctactggaac cactccgggg tcatggatca ggtgtggcgt ttcaacgtcg
    26401 actacaccaa ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca
    26461 gtactgacgg ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg
    26521 ccaccgtttc aaccaagcag ttccaggttt tcagcgaaca gaacaccagt agctactcgg
    26581 cagagccgca gttagacgtt aattactacc agaatgatgt tggtccgttt gatacgcgta
    26641 tttacggcca ggcagtgcac tttgttaaca ccagagacga catgcctgaa gcaacccgtg
    26701 ttcacctgga accgaccatc aatttgccgc tctctaataa ctggggcagc atcaataccg
    26761 aagcgaagtt gctggcaacc cattatcagc aaactaatct tgactggtat aactccagaa
    26821 acacgaccaa gctggacgaa tccgttaacc gcgtaatgcc gcaattcaaa gttgacggca
    26881 aaatggtctt tgaacgcgat atggaaatgc tggctccggg ttatacccaa acgctggaac
    26941 cgcgcgcgca gtatttgtac gtgccgtatc gcgatcagag cgacatctat aactacgact
    27001 catctctgct gcaatctgac tactctggcc tgttccggga ccggacttac ggcggtcttg
    27061 accgtattgc ctccgctaac caggtgacga ccggtgtcac atctcgcata tatgatgatg
    27121 ctgccgttga acgttttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca
    27181 ctggcgatga caacataaca tgggagaatg acgacaaaac gggctcactg gtgtgggcag
    27241 gcgatactta ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt cagtacgata
    27301 cacgtctgga taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc
    27361 gtctggtaca gctgaattac cgttacgcca gcccggaata tattcaggct acgctgccta
    27421 agtactattc cactgctgag caatataaga atggtatttc gcaggtaggt gctgtcgcca
    27481 gctggccaat tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga
    27541 acaagcaagc tgactctatg ttaggtgtgc aatacagctc ctgctgctat gcaattcgcg
    27601 tcggttacga gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca
    27661 acgcaatcgg ctttaacatc gaacttcgcg gcctgagctc caactacggt ctgggtacgc
    27721 aagagatgct gcgttcgaac attctgccgt atcaaaactc tttgtgattt gttgatttac
    27781 cacgtaatcc gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg
    27841 cttctcggta tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa
    27901 gtcgcagccg tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag
    27961 tcggtaaaac tgaacgctgc tcaggcaagg cagcaacttc ctgatgacgc gacgctgcgc
    28021 caccaaataa tggaacgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg
    28081 ggagtgaaaa tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac
    28141 aacatgacgc tggatcagat gcgcagccgt ctggcttacg atggtctgaa ctacaacacc
    28201 tatcgtaacc agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt
    28261 cgtcgcatca ccatcctgcc gcaggaagtc gaatccctgg cgcagcaggt gggtaaccaa
    28321 aacgacgcca gcactgagct gaacctgagc cacatcctga tcccgctgcc ggaaaacccg
    28381 acctctgatc aggtgaacga agcggaaagc caggcgcgcg ccattgtcga tcaggcgcgt
    28441 aacggcgctg atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac
    28501 ggcggccaga tgggctgggg ccgtattcag gagttgcccg ggatcttcgc ccaggcatta
    28561 agcaccgcga agaaaggcga cattgtgggt ccgattcgtt ccggcgttgg cttccatatt
    28621 ctgaaagtta acgacctgcg cggcgaaagc aaaaatatct cggtgaccga agttcatgct
    28681 cgccatattc tgctgaaacc gtcgccgatc atgactgacg aacaggcccg cgtgaaactg
    28741 gaacagattg ctgctgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag
    28801 ttctcgcagg atccaggttc tgctaaccag ggcggcgatc tcggttgggc aacagcggac
    28861 atttttgatc cggctttccg tgacgccctg acccgcctga acaaaggtca aatgagtgca
    28921 ccggttcact cttcattcgg ctggcattta atcgaactgc tggatacccg taatgtcgat
    28981 aaaaccgacg ctgcgcagaa agatcgtgca taccgcatgc tgatgaaccg taagttctcg
    29041 gaagaagcag caagctggat gcaggaacaa cgtgccagcg cctacgttaa aatcctgagc
    29101 aactaatggt taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc
    29161 cggacttagt tgtccagctt gcacagcgtg agtggccggt cgaactggtt gtttgtgccg
    29221 atgccactct ccttaccgac cgggcagcga tgctcggttt gccgctcacc ctccgcactt
    29281 attcccccaa ctctcctgca caaccgcaaa ctgcgggcac attaacgcta cttcctgtcg
    29341 cgctacgtga atctgtcact gcggggcagt tagcggttga aaatgggcat tatgtggtgg
    29401 aaacgctggc gcgagcgtgc gatggctgtc tgaacggtga atttgctgcg ctgatcacag
    29461 gccccgtgca taaaggcgtc attaacgacg caggcattcc gtttaccggt cataccgagt
    29521 ttttcgaaga gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc
    29581 gcgtggcgct ggcaacgacg catttaccgc tgcgcgatat cgcagatgct atcacccctg
    29641 cgcttttgca cgaagtgatt gctattttgc atcacgattt gcggaccaaa tttggtattg
    29701 ccgaaccgcg cattctggtc tgcgggctga atccacacgc gggcgaaggc ggtcatatgg
    29761 gtacggaaga gatagacacc attattccgc tgctcgacga gttgcgggcg caggggatga
    29821 aacttaacgg gccgctgcct gccgataccc tgtttcagcc gaaatatctc gataacgccg
    29881 acgccgtgct ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg
    29941 ggcgcggtgt gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca
    30001 ccgcgcttga actggcggga cgtggcgaag ccgatgtcgg cagttttatt acggcgctta
    30061 atctcgccat caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt
    30121 agcccgtaaa cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt
    30181 ttctgccatt aacccgcaaa agggccaggc aatggtcgaa atcggccccg gtctggcggc
    30241 attgaccgag ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac ttgaccgcga
    30301 tctggcggca cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca
    30361 ggatgcgatg acctttaact ttggcgaact ggccgagaaa atgggtcagc cgctgcgtgt
    30421 tttcggcaac ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac
    30481 tgatgccatt gccgacatgc actttatgtt gcaaaaagag gtggtgaatc gtctggttgc
    30541 aggaccgaac agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact attgcaacgt
    30601 gatcccagtg ctggaagtac cgccgtcagc ctttacacca ccacccaaag tggattccgc
    30661 cgtcgtgcgc ctggttcctc atgcaacgat gcctcacccg gttaaagatg ttcgtgtgtt
    30721 gagccgcatc accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg
    30781 caacctgttt agcgtcgagg tgttaacggg aatggggatc gacccggcga tgcgagcgga
    30841 aaatatctct gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt
    30901 gcaggagagt taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac
    30961 attgaggcac aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata
    31021 cgcaatctgg ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc
    31081 aatggccgtg aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg
    31141 cctggcgaag agtaccagta caccagcggt gcaatcattg aaaccccgct gggcaccatg
    31201 cagggtcact acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta
    31261 ttccgacttg ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc
    31321 gacgttcatg gttgttacga tgaactgatc gcattgctgc ataaagtaga atttacccct
    31381 gggaaagata ccctctggct aacgggcgat ctggtcgcgc gcgggccggg ttcgctggat
    31441 gttctgcgct atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg caatcacgat
    31501 ctgcatctgc tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca
    31561 ccgctgctgg aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctttg
    31621 ctgcaaatcg acgaagagaa aaagttggtg atggcccacg cagggatcac gccgcagtgg
    31681 gatctacaga ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctatc gagtgactcc
    31741 tatcccttct ttctcgacgc tatgtacggc gatatgccaa ataactggtc accggaattg
    31801 cgggggctgg gaagactgcg ttttatcacc aacgctttta cccgtatgcg tttttgcttc
    31861 ccgaacggtc aactggatat gtacagcaaa gaatcgccgg aagaggcccc tgccccactg
    31921 aaaccgtggt ttgcgattcc tggccctgtc gctgaagaat acagcatcgc ctttggtcac
    31981 tgggcatcgc tggagggcaa aggtacgccg gaaggtatat acgcgctgga taccggctgc
    32041 tgctggggtg gtacattaac ctgcctgcgc tgggaagata aacagtattt tgtccagccg
    32101 tcgaaccggc ataaggattt gggcgaagcg gcggcgtctt aaacacagcc tgatatagga
    32161 aggccggata agacgcgacc ggcgtcgcat ccggcactag ccgtaaattc tatacaaaat
    32221 taccgccgct ccagaatctc aaagcaatag ctgtgagagt tctgcgcatc agcatcgtgg
    32281 aattcgctga ataccgattc ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct
    32341 tccacttctg cgtcgatatg cgtcagatac agtttttgcg cttttggcaa gaactgttca
    32401 taaacgcgac cgccgccaat caccatgatt tctggtacgt caccacacgc cgcgatggct
    32461 tcatccaccg acttcaccca cgttacgcga tcgtccgtac ccggttgact gctgaggata
    32521 atatttttgc gtcctggcaa cggacgaccg attgattccc aggtatggcg gcccataatc
    32581 acgggtttat ttaaggtgtt gcgtttaaac caggcgagat cggcaggcag gttccacggc
    32641 atggcgtttt ccatgccgat aacgcgatct accgctaacg ccgcaatcag actgatcatt
    32701 gagatttccc gataaaaaaa attgtcgcca ctatacgtaa agcgtaaacc gtcgtcgact
    32761 ggtgcgagga tgatgttgag gaaaatttta tattctgctg gcgagtccac gctctctccg
    32821 tggactcgcc gcattacaat gaaacaaaaa caaacagtta gctgtaaagt gtgatttacg
    32881 tcactcttta ttaggatgag ggtttcgttt ccggttcatc cgccatgttg ccggtatgtt
    32941 taccttcttc ggttccctgc cagccatgtc gttgaattaa tgacagatgt tcgcggtcct
    33001 cggtaatgat ctcacttaac atcgcgctgg tgcgtttata gaccgccgcg cgggctttgg
    33061 tgtcgttctc aaccattgcc atctcttcca ccatctgaat attaaagcgg cggaacacat
    33121 cggcacgttc tcgcgcttca tacggcccca gacctaaact ttccagtgcc agacgcccgg
    33181 ttttcagcgc accttcgaag gtttcacgct ccggcttttc aacgcctgcc tgacgcaaac
    33241 gaatgtagtg gtcgacatcg cgggcgcggg caataatctg caaatgcggg aaatgttctt
    33301 tcaccatctc tgtcagttgc aggttggttt gcggatcgtc gatggcgtta atcagcactt
    33361 ccgctttcgc cgctccggca gattccagta aatccatccg cgtggcatcg ccataaaaca
    33421 ctttcatacc aaatttacgc aaggtttcga tatggtccgg atcgtgatcg agtaccacca
    33481 ttttcacccc gctggagagc agtaaacgtc cggtaatctg cccaaaacga ccgaatccgg
    33541 cgataatcac gcgcggctgt tcttcgtcga tctcatcggc ttcacgcgct tcctcagtag
    33601 aagattgctc aaggcgattg aggatcacca gcagaatcgg cgttgctgcc atcgacagcg
    33661 ccaccgccag ggtcagcgat ttcgcccact ccggctccag cacattcgcc atctgcgccg
    33721 cgccaaatac cacaaaggca aactcactgc cctgccctaa caacaccgca aaccaacgac
    33781 gctgtttatt tggcacttgc aacggtcggg caatcagcca cagcatggcg attttgatga
    33841 tgaggaaacc gagcagcaaa atgacaatgc gcaatgggtt ttcaagcagc gtgccaaagt
    33901 ctatcgacat gccaacaccg atgaaaaaca gccccaacag caaaccttta aatggttcga
    33961 tatcgctctc cagcgcatga cggtattccg agcttgccag cagtacgccc gccagaaacg
    34021 cgcccatcgc catcgacaag ccgacctctt ccagcagcaa accaaagcca aacacgagga
    34081 ataacgccac ggcactaaac acttcccgca agccagagcg ggctacaaaa cgcagcgccg
    34141 gacgcgtgac atagcgcccc agcaatacca ccagcaccag cgcacccgcc acttttaacg
    34201 ccgagagagc aaatgcgccc atcgtcgtcg aggcactgct cgttgccagt agcggaatca
    34261 tcgccaccag cgggatcgcc gcgatatcct ggaacagcag caccgcaaag gcactgcgac
    34321 ccatttgcgt caccatcaga ttgcgttcat tcatcgcctg catggcaatc gccgtagagg
    34381 agagcgccag cgtcatgccg atcaattccg cgacctgcca gcgcagccca agtaacatgc
    34441 agaacagccc cagcaggcca ccgcaaatca ccatctgcaa tgcgccaccg ccgaacactg
    34501 ccgcacgcag cttccacagc ctttgtggat cgagttcgag gccgataata aacagcatca
    34561 gcaccacgcc aatctcggca aagtgcagaa tagattcggc atcggtcacc agtcgcagcc
    34621 cccacgggcc aataatgcag ccggcgatca ggtagccaag taccgatccc agaccaagac
    34681 gtaccgcaat gggtacaatc agcgctgccg aaccgagata aatcagcgcc tgaatcagcg
    34741 tatggctatc catgatgggc ctcctgccat tccagcagac gttgcttata gtgacgcgcc
    34801 tgcccttcga gggtttcgtc gtcacaaata aaggtgcagt gcatggcaaa cggtggcagc
    34861 cagttcagcc cgcagtagat tgccgtcgcc tgtagcggct gcgacagcac atcaaagccc
    34921 ggatgcgcac caatttcaaa atggctttcc ccgccgccgg tcgtcaccgc ccacagcaaa
    34981 tgtttgccat gcagcgccgt gccgccgtga ccgtaagccc agccgtgcga gaaaacttta
    35041 tcgatccaaa gtttgaggag cggtggaatg ctgtaccact gcatcgggtg ctgccagacg
    35101 atcagatcgg cgcgagacag cgcctcctgc tcggcggcaa tatcgatatt gaagtcagga
    35161 tagagttgat aaagagagcg aatttcgacg ccttccagcg tccttgcctg ttcaagcatc
    35221 cgtttattcg catgggaatg atgcggatac ggatgcgcat aaattataag aatcataggt
    35281 agcccgcgtg attacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    35341 tagtcaataa tattgattca gaaaagagag aaatctgaac aaattacccc attctctgcc
    35401 cgcggatcgt catattgctg gtctgcgcca gtgacatccc tcgagtctcc ggggcaaacg
    35461 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    35521 taccgtaatt attgataaag atcggcagtg cccaggtcga aacaatggtg ccaatacggc
    35581 ttaaggacat aatcacgccc acggcagagg cgcggatatc tgtcgggaag agttcattag
    35641 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    35701 tcactaccag ccagatcccc atatccggga tcagccccaa aaccgccagc gccagcgtca
    35761 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttaac cacagcatcg
    35821 gcggaataca gccgagcata aagaacaggc taatcaccac gttccccagt gccgcgtttt
    35881 tgccaacccc caatcccaac aaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    35941 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    36001 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    36061 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    36121 agcggggaga ttcaggcagt tcgaatcgac caatcaaaat caacaaacag gggatcgccg
    36181 cgctacccag catccagcgc cagccgcctt ccacatcata aagccagtag ccgaccagat
    36241 cggcgcaggt cgcgccgaca taccacatgg cggcgataaa gctgatggaa aacgcccgct
    36301 gacgggtact ggagaactcg gtgatcattg aggtggcgat gggataatct gcaccgatga
    36361 caatgccgat aagtacccgc atcaccaaca gttcgacagg ggatgagaca aacatcgtcg
    36421 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    36481 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcac
    36541 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    36601 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    36661 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    36721 attcctgttt tttgcaatga gagtcagtgc ggcgttacgt atcaaaccaa catcagccgt
    36781 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    36841 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    36901 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagcacct
    36961 gtttatcagc atcggccttc acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    37021 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    37081 gccatccttg attagattga tgaagcccac tttcttaccg tggcggagga ttttcttgcg
    37141 catcagttct ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    37201 ctccgggtag ccgctgaaca tgcgtgggtt atcaaggaac gccggtagtt tctggtacat
    37261 gcgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    37321 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcagctt ccccggcggc
    37381 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    37441 gccggcaatc aatacgccgt caccgaccag ctccggcagc atgttgatgc ctgcttccgg
    37501 cactacgtga gcggaatatt ccaccagctt gccacccgcg atcagcggtg caacggccgg
    37561 atgttgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    37621 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    37681 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    37741 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    37801 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    37861 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    37921 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    37981 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    38041 acgctgggat ggcgaggttt cgtcaccatt gcagtagtcc atagtcatcg ctgacttttc
    38101 cgtcataaac gcgagttttt catgggtgat caggcgttct acgggggcgg agtcggcgaa
    38161 accaggaata atgtgttcta ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    38221 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    38281 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    38341 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    38401 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    38461 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    38521 ttagcgccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    38581 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    38641 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    38701 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    38761 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    38821 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    38881 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    38941 gccagaccac cgtaaaccat gtgtttcact gtcgctttac cgtcctgtac gctgacggtg
    39001 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    39061 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    39121 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    39181 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    39241 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    39301 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    39361 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    39421 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    39481 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    39541 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    39601 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    39661 cagtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    39721 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    39781 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    39841 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agcacatctt
    39901 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    39961 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    40021 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    40081 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    40141 aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    40201 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    40261 gttaagttcc tcaccaatat tgaaaacatg gcgtagcaaa aagaaatttt caatattgct
    40321 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    40381 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aacaaaaatg
    40441 tgataacaat cacagaatgc agcttattga atacccatta tgagttagcc att
//
LOCUS       DAFNBJ010000022        39878 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000022 DAFNBJ010000000
VERSION     DAFNBJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.022"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..274)
                     /gene="chbB"
                     /locus_tag="L2X49_001171"
     CDS             complement(<1..274)
                     /gene="chbB"
                     /locus_tag="L2X49_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBN4796285.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLG"
     gene            complement(573..911)
                     /gene="osmE"
                     /locus_tag="L2X49_001172"
     CDS             complement(573..911)
                     /gene="osmE"
                     /locus_tag="L2X49_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBN4796286.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1113..1940
                     /gene="nadE"
                     /locus_tag="L2X49_001173"
     CDS             1113..1940
                     /gene="nadE"
                     /locus_tag="L2X49_001173"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBN4796287.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELRDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2170..3057
                     /gene="cho"
                     /locus_tag="L2X49_001174"
     CDS             2170..3057
                     /gene="cho"
                     /locus_tag="L2X49_001174"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBN4796288.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3017..3592)
                     /gene="ves"
                     /locus_tag="L2X49_001175"
     CDS             complement(3017..3592)
                     /gene="ves"
                     /locus_tag="L2X49_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBN4796289.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3795..4280)
                     /gene="spy"
                     /locus_tag="L2X49_001176"
     CDS             complement(3795..4280)
                     /gene="spy"
                     /locus_tag="L2X49_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBN4796290.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4610..5578)
                     /gene="astE"
                     /locus_tag="L2X49_001177"
     CDS             complement(4610..5578)
                     /gene="astE"
                     /locus_tag="L2X49_001177"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBN4796291.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5571..6914)
                     /gene="astB"
                     /locus_tag="L2X49_001178"
     CDS             complement(5571..6914)
                     /gene="astB"
                     /locus_tag="L2X49_001178"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBN4796292.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAMMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6911..8389)
                     /gene="astD"
                     /locus_tag="L2X49_001179"
     CDS             complement(6911..8389)
                     /gene="astD"
                     /locus_tag="L2X49_001179"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBN4796293.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8386..9420)
                     /gene="astA"
                     /locus_tag="L2X49_001180"
     CDS             complement(8386..9420)
                     /gene="astA"
                     /locus_tag="L2X49_001180"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBN4796294.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9417..10637)
                     /gene="astC"
                     /locus_tag="L2X49_001181"
     CDS             complement(9417..10637)
                     /gene="astC"
                     /locus_tag="L2X49_001181"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HBN4796295.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11083..11889
                     /gene="xthA"
                     /locus_tag="L2X49_001182"
     CDS             11083..11889
                     /gene="xthA"
                     /locus_tag="L2X49_001182"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBN4796296.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12056..12766
                     /locus_tag="L2X49_001183"
     CDS             12056..12766
                     /locus_tag="L2X49_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBN4796297.1"
                     /translation="MNAERKFLLACLIFALVIYAIHTFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LRRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASVLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12771..13448
                     /gene="ydjY"
                     /locus_tag="L2X49_001184"
     CDS             12771..13448
                     /gene="ydjY"
                     /locus_tag="L2X49_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBN4796298.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13463..14170
                     /locus_tag="L2X49_001185"
     CDS             13463..14170
                     /locus_tag="L2X49_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBN4796299.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14170..14718
                     /locus_tag="L2X49_001186"
     CDS             14170..14718
                     /locus_tag="L2X49_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBN4796300.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14728..15894
                     /locus_tag="L2X49_001187"
     CDS             14728..15894
                     /locus_tag="L2X49_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN4796301.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15867..17402
                     /locus_tag="L2X49_001188"
     CDS             15867..17402
                     /locus_tag="L2X49_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBN4796302.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLKDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17402..18055
                     /locus_tag="L2X49_001189"
     CDS             17402..18055
                     /locus_tag="L2X49_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN4796303.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18122..19429
                     /gene="ynjE"
                     /locus_tag="L2X49_001190"
     CDS             18122..19429
                     /gene="ynjE"
                     /locus_tag="L2X49_001190"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HBN4796304.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19438..20058)
                     /locus_tag="L2X49_001191"
     CDS             complement(19438..20058)
                     /locus_tag="L2X49_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBN4796305.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            20145..20552
                     /gene="nudG"
                     /locus_tag="L2X49_001192"
     CDS             20145..20552
                     /gene="nudG"
                     /locus_tag="L2X49_001192"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBN4796306.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLESFMALRAARPAD"
     gene            complement(20518..20790)
                     /locus_tag="L2X49_001193"
     CDS             complement(20518..20790)
                     /locus_tag="L2X49_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBN4796307.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            21026..22369
                     /gene="gdhA"
                     /locus_tag="L2X49_001194"
     CDS             21026..22369
                     /gene="gdhA"
                     /locus_tag="L2X49_001194"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBN4796308.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIASFVKVADAMLAQGVI"
     gene            complement(22486..23526)
                     /locus_tag="L2X49_001195"
     CDS             complement(22486..23526)
                     /locus_tag="L2X49_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBN4796309.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGG
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(23654..25615)
                     /gene="topB"
                     /locus_tag="L2X49_001196"
     CDS             complement(23654..25615)
                     /gene="topB"
                     /locus_tag="L2X49_001196"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBN4796310.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPEAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(25620..26663)
                     /gene="selD"
                     /locus_tag="L2X49_001197"
     CDS             complement(25620..26663)
                     /gene="selD"
                     /locus_tag="L2X49_001197"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBN4796311.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(26780..27331)
                     /locus_tag="L2X49_001198"
     CDS             complement(26780..27331)
                     /locus_tag="L2X49_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBN4796312.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIGKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            27492..29348
                     /gene="sppA"
                     /locus_tag="L2X49_001199"
     CDS             27492..29348
                     /gene="sppA"
                     /locus_tag="L2X49_001199"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBN4796313.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            29515..30531
                     /gene="ansA"
                     /locus_tag="L2X49_001200"
     CDS             29515..30531
                     /gene="ansA"
                     /locus_tag="L2X49_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBN4796314.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            30542..31183
                     /gene="pncA"
                     /locus_tag="L2X49_001201"
     CDS             30542..31183
                     /gene="pncA"
                     /locus_tag="L2X49_001201"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBN4796315.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(31344..31616)
                     /locus_tag="L2X49_001202"
     CDS             complement(31344..31616)
                     /locus_tag="L2X49_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBN4796316.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(31658..32071)
                     /gene="msrB"
                     /locus_tag="L2X49_001203"
     CDS             complement(31658..32071)
                     /gene="msrB"
                     /locus_tag="L2X49_001203"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBN4796317.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            32413..33408
                     /gene="gapA"
                     /locus_tag="L2X49_001204"
     CDS             32413..33408
                     /gene="gapA"
                     /locus_tag="L2X49_001204"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBN4796318.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            33492..34376
                     /locus_tag="L2X49_001205"
     CDS             33492..34376
                     /locus_tag="L2X49_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBN4796319.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(34427..35281)
                     /gene="yeaE"
                     /locus_tag="L2X49_001206"
     CDS             complement(34427..35281)
                     /gene="yeaE"
                     /locus_tag="L2X49_001206"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HBN4796320.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTSLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHHGVMAIPKAATIAHVQQNAAALEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(35371..36117)
                     /gene="mipA"
                     /locus_tag="L2X49_001207"
     CDS             complement(35371..36117)
                     /gene="mipA"
                     /locus_tag="L2X49_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBN4796321.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            36553..38487
                     /gene="yeaG"
                     /locus_tag="L2X49_001208"
     CDS             36553..38487
                     /gene="yeaG"
                     /locus_tag="L2X49_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBN4796322.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            38600..>39878
                     /locus_tag="L2X49_001209"
     CDS             38600..>39878
                     /locus_tag="L2X49_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HBN4796323.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAK"
BASE COUNT         9652 a        10464 c        10023 g         9739 t
ORIGIN      
        1 cgcctaaacc atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg
       61 gtaacaaacg ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat
      121 cggcattctg acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa
      181 cttcatattt ttctgcctgt gcgcgcattt ttgataccag taaagaggta gacatgcccg
      241 cagaacaaaa cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct
      301 gtcagccaga cactccgcaa gccttaacct gcttccatgc tctgggtaac ttgcgaaacc
      361 aacatgactt tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc
      421 tttgacctta taaattgttt ctccttgacc tgtggttatg accctcttca catttcgggc
      481 aaataattcg cgaagataat taagaactca ggccaaaaaa aaccggcgca atggccggtt
      541 tccgttgtta ctcaagcttt cagacgaatt gattacttcg cagcctgtgg atcagtgtcg
      601 tattcagcac aggtctgata accggagttg atgacatgtc cggtatcatc taacgcgaca
      661 aagtaggttt ctgctttacc atcacgttga cccaggatgt aggtctggca agtaccgcga
      721 gcatggatca tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac ctgcgcccgg
      781 ctcatgcctt ttttgacgtc tttcaccaca ggctgtacaa actggtcttt ggtacgatca
      841 taagccgtac aacccgccag catggttaat accgccgctg cactcagaat tcctgccata
      901 ttcttgttca tattccgtcc tcttgtttat cagcgtgtta gataagcctg gaatacattg
      961 ggcgcttttt caagcccgtg aacgaaacgg ctccgctttc agaggattcc tgtatgacgt
     1021 tttaaccacc attcagcccg ctgtcgcttg tcgtttcagt agcaacgggt tagctttaag
     1081 gaagttttgt cttttctgtc tggaggggtt caatgacatt gcaacaacaa ataataaagg
     1141 cgctgggcgc aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt gtcgattttc
     1201 tgaaaagcta cctgcaaact tatccgttca ttaaatcact ggtgctcggg atcagcggcg
     1261 gtcaggactc cacgcttgcc ggaaagctgt gccagatggc gattaatgag ctgcgccagg
     1321 aaactggcaa cgaatcactg caatttattg ccgtacgcct gccctatggt gttcaggctg
     1381 acgaacaaga ttgccaggat gccattgcct ttattcaacc ggatcgcgta ttaaccgtta
     1441 atatcaaggg cgcggtattg gctagcgagc aggcattgcg ggaagcaggc attgaactgc
     1501 gcgattttgt ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa tatagcattg
     1561 cgggtatgac cagcggtgtc gtggtgggca ccgatcatgc agcagaagcc attaccggat
     1621 tcttcactaa atatggtgac ggcggtacgg atattaatcc gctgtatcgt ctcaacaaac
     1681 gccagggtaa acagttgctg acggcattag gttgcccgga acacctttat aagaaagccc
     1741 caacggccga tctggaagat gatcgccctt ctctgccaga tgaagtggca ctcggcgtga
     1801 cctatgacaa tatcgacgac tatctggaag ggaaaaacgt acctgaacag gtcgccagaa
     1861 caatagagaa ctggtatctg aaaaccgaac ataaacgccg tccgccaatt accgttttcg
     1921 atgatttctg gaaaaagtaa taatttgcac ataatggttt gtgcgaaaaa gagtaatttg
     1981 ttcacgccgg atgcggcgtg aacgccttat tcgacctaca aaactatgca aattcaatat
     2041 attgcaggag tcgaataggc ctgacaggcg tagcacgtca gacggtgtaa cctttgtcat
     2101 cgacccgcct cttttttaat cgcttcccgc ctgttacact ggatagataa ccagcattcg
     2161 gagtcaacag tggtacggcg tttaacttct ccgcggctcg aatttgaagc tgcggcaatt
     2221 tatgaatatc ccgaacattt acgttcattc cttaatgact tacccacccg accaggggtg
     2281 tatctgtttc atggcgaaag tgacaccatg ccgctctata tcggcaaaag cgttaatatc
     2341 cgcagtcggg tgctttctca tttacgcacc ccggatgaag ccgccatgct acggcaatcc
     2401 cggcgaatta gctggatctg caccgcaggc gaaatcggcg ctctgctcct tgaagcgcga
     2461 ttaatcaaag aacaacagcc gctgtttaat aaacggttgc gccgcaatcg ccagctctgt
     2521 gccctacaat taaatgaaaa gcgcgtcgat gtggtgtatg ccaaagaggt ggatttttca
     2581 cgagccccca acctgtttgg cctgtttgcc aataggcgcg cagctttgca agcattgcag
     2641 agcatcgctg atgaacaaaa actttgttat ggcctgctgg gactggaacc gttaagtcgc
     2701 ggtcgtgcat gttttcgttc agcgctaaaa cgttgcgccg gagcatgctg cggtaaagag
     2761 agccatgagg aacatgcgct acgcttgcgc cagtctctgg agcgtttgcg ggtggtgtgt
     2821 tggccttggc aaggggcggt ggcgctgaaa gaacagcacc cggaaatgac tcaatatcat
     2881 attattcaaa actggctgtg gctgggggcg gttaattcgc tggaagacgc gacaacgtta
     2941 attcggacac ccgccgggtt tgatcacgac ggttataaaa ttctttgtaa gccgctgctt
     3001 tccggtaact atgaaattac tgaacttgat ccggcgaatg accagcgagc cagttgattt
     3061 cactgaacaa cagtttacct tgcggttgta aaagccgcag ggtgtgcctg ccatcgaacc
     3121 agcacgcgcc ctggtcggta gtgagcaatt tatcgcccag ttgccacgcc ccgttaatca
     3181 caaacaccac cccgccgcgt gagccaaagg tagtaaacgt acgttcggca attctgactt
     3241 tcgccttaca gacatccagg cgtgtcatga tgttgaaatc catcgacatc tgccctgccg
     3301 tcagtttcgc ttttaccacc tggtccgctg caaaggcaaa aggctgtaac ggttttaagg
     3361 tatggttaaa gcggtctgcg ctttcaagga gcatctcgcc gccttccagc aacgtcacta
     3421 tcctttccat gccgggaaac aaagaaaact ccccattcgc cgcgatggat gcaatgctgg
     3481 cacgccagta aaaatcacgt tttgccggtg ggaaagtgca aatttcgcgc gtttcaccgg
     3541 cagcatttcg ccacagattc accgacattt tacgcatatc aaagtattcc atactcgctc
     3601 ccggcccgtt cttttcattg ttatcgttca ggtaaccgtg gatggttatc ggcaactcgg
     3661 gtgatttacg ttagtggtga tcaggctttt tttacaactg tttgagaaga gaaaagaaaa
     3721 ccgccgatcc tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt
     3781 ttttggctga aagattattc agcagttgca ggcattttac cttttgccgc tggacgttct
     3841 gtcagacgct tctcaaaatt agcattaaat tgctttttct gttccggcgt caggatgttg
     3901 taaattttgt tctgggtttc catgtgcgcc agcatgttag ctttgcgctg ttcttccatt
     3961 tttgcgatct gcgcttcagc ttttgcttta tcgaaggtat cgctggcaat gatgtcatgc
     4021 attgcgcggc gttcttccag cggcggacgt ttcatctggt cacgctggcc tttcatgatt
     4081 tcgcggatct gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat catgtcctga
     4141 tgcggaccga acttgccttt gtggtgcatc atcggcttcg cgtcagccgg tgctgcggta
     4201 gtggtgtctg cggcatgggc caggttagcc gcgccaagag ccagggtaga ggcaacaaac
     4261 agtgcagtta atttacgcat attctatatc cttcctttca gttatttatt acggctttct
     4321 taagtagcgt gccgtgttga cgagattaac tttactgact ttagcgtcaa ttaatcagag
     4381 caacggtaaa acaatgaaag tgtaaaaaac acttttgcgc caattatgga gaaaaaaaga
     4441 aaatttgatg gagagtgatg agagaataat acaacacgat gattttgcag agattatgaa
     4501 gaactatacc ggatgactgg tgataaataa agcaaataac caggattaat ctgtattaat
     4561 ttataagaaa gcaacttaat acccgcagaa tgatttctgc gggtaagtat tagcttattt
     4621 tttcgagcat taatcccgcg cgtaatccca acgctaccaa cggattaggg aataacacat
     4681 actctacatc atgggttacg gtaaaacgtt cctctccgtc ctgcgccagc aatgttcctt
     4741 tctcaaacgg cataaaattc agcgtgtcac ttgccatatg catttcgaag gacggcgagt
     4801 ggcgagtaat ttgcgaaacc acccgataac ggagcggcgg tgttctcacg ataccgacac
     4861 tctcaccaga tagcagcgca gcaattgcgc tggcagttac tgcaaactgg cgaagatcgt
     4921 tttgcccaaa gggcaacgct ttgccaagtt ccagcgtaca ggccagcgcg ccaaaatgtc
     4981 tggcgctgaa atgggtaaac gtaccaccag gttcctgatg gaacaccagc gcctccagcc
     5041 ccgccgcacc cagccacgtc agaaatttct cgtcccaggg aatgtcgcgt tgcggtaata
     5101 caccgaactg cggatgcaag gagccacgaa ttgcggtatg tagatcaagg tgccagcgca
     5161 cagattcttt gccctggtca taaaaatctt ccaggcactg ttccagttcg cgcgcccgac
     5221 aggtttctcc gctttcagca aatagctgcc aacgaccgcc aaacattcga ttcatatcgc
     5281 tatggcaata acgtttccct tgcttcagcg caggaggatt cccgaggatc accagcaacc
     5341 gccaacgtaa cgggatctcg ccgtgagata tcgcgccaag caacgcgtcc agcatctcca
     5401 caggtgccgt ctcattaccg tgtattcccg ctgaaatcac cagtgcgcct tgcggtggcg
     5461 ttaatggcgt cagttccagc acaccatcgc ccagccagcg ccagcgaacg ccgttgattt
     5521 ctcgctcggt gataaccggt tttttacccg ttaaggtcag agcaagaaaa ttatccattg
     5581 ccccctccct cgcgctggaa cggataaacc gaaccgagat tcagtaattg gctcaataca
     5641 tccagtgctt cccgcccttc gcgcagcaat tgcgggtcgg ccagatcggc agcagtaagg
     5701 cgatcgcggt agtaacgatc cacccagtca ttgagcgcat taaacagcgt atcgttcatc
     5761 atcatcgccg gattcaccgc ccggcgttct tcttctgtca ataccacccg caaccgcagg
     5821 cacgccgggc cgccgccatt cgccatgctt tcacgtaaat caaagacttt tagttcgcta
     5881 atcgggttgt cagcggcaag gagttcattg agataacccc atactccggc gtgttcccga
     5941 cactcctgag gcagcaccaa catcatggaa ccatcatcgc ggctcaacag ttggctgtta
     6001 aacagatagg tagacaccgc atccgacacg gaaacctgag ttgccggaac ttctatcgcc
     6061 ataaaaccgt tcacccgcgc acgcaggttt gccagtaact gtgactggcg agcgaacgcc
     6121 tgttggtggc aaaacagcac ctggcggtta ctcacggcaa tcacgtcatt atgaaaaacg
     6181 ccctggtcga taacgtccgg gttttgctgg gcgaaaatca cctgttgggg attcacctga
     6241 ttcagccttg ccaccgcctc gctggcttcg cgagtctgtc gcgccggata acgggaaggc
     6301 cgggtatcat tgccttcttc tcgcccgtag acaaaaagtt gcatacccgg ctcaccgtaa
     6361 tgaccgccga gacgattgtg gtttgccgcc ccctcatcac cgagcaacgc tacctgcggc
     6421 aacgccgaat gaacgctaaa tttctcttcg tcgttaaaaa tcgcttttaa aagtgattcg
     6481 gtgacgtgcg cttccagcga gcggtgaaat ttattgttca ggttggcaac ggtgagatgc
     6541 actttgccat ccagcgtatc ggcagatggc gcgatcgttg ccgcattggc tacccacatt
     6601 ggcgaagcgg aactgacgct ggaaagccag tgcggtgcct ggcgtgcaac tttttccagt
     6661 acctgctcat cgctaccact gaatcccaac tgacgcagca ccggaataaa cggacgctcg
     6721 tgcggcggga tcacggcctg ggggaatccc gcatcggcaa gggctttcat tttcagtaag
     6781 ccctgttttg ccgcctgtcg cgggttagac acctgaaaac ggtgacgggt agaggcttca
     6841 ttaccaaacg acaggccagc gtaatgatgc gtcagcccta ccagcccgtc gaaattaact
     6901 tcccaggcgt tcatcgcacc acctcatcgg aaaaatccag cccggggtta agcgttgcgg
     6961 gcaaagttaa cgagtccgac tccaggctcg ccataggcca tgcgcagtaa tctgcggcat
     7021 accaggcgct ggggcgatgg ttaccggatg caccaatgcc gccgaatggc gcggtactgg
     7081 cagcaccagt aagcggtttg ttccagttaa caatccccgc ccgcgcctcc agcaacagtt
     7141 gatcgaattt ttcccgctcg ggggaaacca gaccgcaaga gagtccgaag cgagtgttat
     7201 tcgccattag aatcgcttcc tcgaaagaat cataacgcca gacgcgcaat aacggtccga
     7261 acacctcttc atctggtacg ccagcaacgc ctgtcatttc aatgatcccc ggcgtcagca
     7321 acgatgtctc tgattgtaat aagcgcggcg caagcagggt tcgtccgccc atcgcttcca
     7381 gttgctgcca ggcagtaacc acctgctgtg cggcctgttc agaaatcagc ccgccaataa
     7441 acggctgcgg ttcgtcatcc cagttgcccg gcgttaatcg ctggctgacg gcaaccagac
     7501 gagcaagaaa cgcatcgccc tgcgctccgc ttttgagcaa taaacggcgg gcgcaggtgc
     7561 agcgttgccc ggctgtgaca aacgccgact gaatggtcag atggacagcc gcgtcgatat
     7621 ccgccacctc atcgataatt agcggattat taccgcccat ctcaagggcg agaattttct
     7681 ccggctgacc ggagagctgg cgatgcagct ggtagccggt attggcgcta ccggtaaaca
     7741 gcaaaccgtc gagatcctcc agcgcactca gcgcctgacc cgtttcacgc ccgccctgca
     7801 ccaggttaag tacgcccggc ggcaagccag cctgctgcca taaacgcatt accgcttcgc
     7861 cactccacgg tgtcagttcg ctgggtttaa agataatggt gttacctgcc agcaatgccg
     7921 gaacgatatg tccgttcggc aaatgaccag ggaaattata cggcccaaac accgccagca
     7981 cgccgtgcgg gcgatgtcgc aggctcgccg caccgtccgg catttcacta cgctgctcgc
     8041 cggtacgaac gtgatacgcc ttaattgata tcgcgatttt attgatcatc gccgtcactt
     8101 cggttgccgc ttcccagcgc ggcttacccg tttctctggc aataatcgcg gttaattcgc
     8161 ctttattcct ttccagcaat ccggcaaagc gttcgacaac gacctgacgt tcagccaatg
     8221 agagccgcgc ccagcgcgga aacgccgcac gggctgcgcg acaagcctgc ccgacctgag
     8281 cggcatcggc atcattgcct tgccataaca cctcgcccga taccggatta cgcttcacac
     8341 gcgatgcgcc ctggcccgtt atccagtcac cgttaatcca taaagtcatg ctgttttctc
     8401 ctctgcgcac aggcgcacca gacgaacgcg atcccctgcg tggcatttga gggcatccag
     8461 ttgtgcggcg gttaaaatca aacgctcggt tgccggatcg gtacgcgcca gcaccacgcg
     8521 gaaatggtga taattttcat tggcgaccag gcaggccgga aaatcgccct gcgcaggctg
     8581 cccttctgcc acttccacca gccgactttt acgaatggcg cgcacgcggt cgatgtcaca
     8641 ctcaagcgtt ggcccgccgt cgaagatgtc gatatagttg cggtaacgaa aaccttcttt
     8701 ctccagcacc gcgcgggcag gcgcggtttg cggatgtacc tgaccgatga cgtcctgggc
     8761 ttcctgggat aaaaagtggg tatagatcgg atgtttcggc atcagttctg caataaatgc
     8821 cttttgcccg gtgccgcaga gaaaatcggc gcggctaaaa tccatcgaaa agaagcgttt
     8881 accgaggctt tgccagaacg gtgaatagcc gtgttcgtca atcaccccgc gcatttcagc
     8941 aaccactttg tcattaaact tgtcgcgaaa agccgccata aacataaagc gcgatttcga
     9001 cagcaaatag ccgttgccct ctttgcgcca gtccgggtcg agaaacagcg tgcacagctc
     9061 gctgctgccg gtgtgatcgt tactgagaaa cagcgtcggc aatgcgttat agacattcag
     9121 ctcttttgag gcgtgaacca acgtgccgac gcgatagttg taccagggat cgttcagccc
     9181 aaccgccacc tcaatggcac aaatccccgc cacggtgcct gtctcgctat cttccagcac
     9241 gaacacatag ccctgctcac ttttgggcag ttcgccttgc caggttttga ttgccctttc
     9301 gatacgcgcc gaaagcgtgg cttcattggc gggaagcgac gtcaggccgc cgcccgtttt
     9361 gctggcaagc tgcatcagcg ccgagacatc tgatcgctca acgggacgga tgaccatcat
     9421 gatgaacctc ggctaacaaa gtgttcgcaa gcagctgcaa agcgatccag tccggtcgtc
     9481 acctcttctt cgctgacatt gagcgcaggc gcaaaacgca ccacgttgcc acccgcaatc
     9541 agtaccatca cgcctgcttt cgccgcttcc tgagagatct gtttcgcttg cccggcgtaa
     9601 tcggcattca gtacacagcc aatcagcaaa cctaagccgc gaacttcact gaacaaacca
     9661 tagcggtgat taatagtatt aagacgctca acaaaccagt cgtgacgctg tttaacgcca
     9721 ttaagcatct ctggtgtgtt gatgagctcc agcactttgc ctgccaccgc cgaggccagc
     9781 ggattaccgc cataggtggt gccatgagtg ccaacggtca tcacgcgggc gcactcttcg
     9841 gttgccaaca acgcaccgac cgggaaaccg ccgcccagcg ctttggcggt agttaacaga
     9901 tcaggcgtca cgccgtagtg catataggca tacagttccc cggtgcgccc gacgccggtt
     9961 tgtacttcat caaaaatcaa cagcgcattg tggcggttac acaattcacg cagaccttgt
    10021 aaaaacgcgt tgctggctgg caccacaccg ccttccccct ggatgggttc gacaatcacc
    10081 gcacaggtag agtcgtcaat cagcgcgctg gcagagttaa tatcgttata tgcagcatga
    10141 cgaatatccg ccggcagtgg cgcaaaatcc tgtgaatagg ctggctgccc acccgcactg
    10201 acagtaaaca gcgtgcgacc atgaaacgca tttttgaacg ccacgatgcc gctcttatgg
    10261 ctgccgtagc ggtcgtgagc gaatttacgc gccagtttta gcgccgcttc gttggcttcc
    10321 gcaccggagt tacaaaagaa gacgcgatcg gcaaacgtgg cgtcgatcaa ttttttcgcc
    10381 agtcgcagta ccggctcgtt ggtgtaaccg ttgccggtat gccagaactt actcgcctgt
    10441 tcgttcagcg cttcacgcag ttccggatgc gcatggccca gcgcgttcac cgcaatgcca
    10501 cccgcgaagt cgatatactc tttcccctgc tgatcccaca agcgcgaacc ttcgccacgt
    10561 accggtataa agggtgccgg agcgtaaaca ggtatcatcc attcatcaaa gttttcacgc
    10621 gtaattggct gagacatagc gacctctaca gtaaataatt cgttatttat atgttaacaa
    10681 taagtaatat ttgcgctgta aatgtagatt gcagggttcg tgccagccag tgataaaagt
    10741 gcataaacgg cggaggctaa ctggaaatca aggagttata accaaaccat atgcatttaa
    10801 agtgcatata aagtgaatac gtttgcgatg tgggtgaata aaaagaataa aaaacgcaat
    10861 gttatgcaga agtaaaatat aattctggaa ttgtgatcat tgacgaaatt tactggaaat
    10921 tactgcgcca ttctgacgca gcgcgcacca aaagcgggca ttttttgcgc catcgttgac
    10981 atcattaaca accatcgatc aaatcactta acaacaggcg gtaagcaacg cgaaattctg
    11041 ctaccatcca cgcactcttt atctgaataa atggcagcga ctatgaaatt tgtctctttt
    11101 aatatcaacg gcctgcgcgc cagacctcac cagcttgaag ccatcgtcga aaagcaccaa
    11161 ccggatgtga ttggcctgca ggagacaaaa gttcatgatg atatgtttcc gctcgaagag
    11221 gtggcgaagc tcggctacaa cgtgttttat cacgggcaga aaggccatta tggcgtggcg
    11281 ctgctgacca aagagacgcc gattgccgtg cgtcgcggct ttcccggtga cgacgaagag
    11341 gcgcagcggc ggattattat ggcggaaatc ccctcactgc tgggtaatgt caccgtgatc
    11401 aacggttact tcccgcaggg tgaaagccgc gaccatccga taaaattccc ggcaaaagcg
    11461 cagttttatc agaatctgca aaactacctg gaaaccgaac tcaaacgtga taatccggta
    11521 ctgattatgg gcgatatgaa tatcagccct acagatctgg atatcggcat tggcgaagaa
    11581 aaccgtaagc gctggctgcg taccggtaaa tgctctttcc tgccggaaga gcgcgaatgg
    11641 atggacaggc tgatgagctg ggggttggtc gatactttcc gccatgctaa tccgcaaaca
    11701 gcagatcgtt tctcatggtt tgattaccgc tcaaaaggtt ttgacgataa ccgtggtctg
    11761 cgcatcgacc tgctgctcgc cagccaaccg ctggcagaat gttgtgtaga aaccggcatc
    11821 gactatgaaa ttcgcagcat ggaaaaaccg tccgatcacg cccccgtctg ggcgaccttc
    11881 cgccgctaat ttatcagctc tcctggctca aactgggtca ggagaattaa ccttgagaaa
    11941 aatcaacaaa ctgtcagtaa tgatttgttg cctgccgtcc tttgttatac cgtcactgcg
    12001 tttttagttg tctgaccact tctctattat caagtttgat ataggaaact ccacgatgaa
    12061 cgctgagcgt aaatttcttt tggcctgtct tatttttgcg ctggtcattt acgctatcca
    12121 cactttcggt ttattcgatc tgctcaccga tttaccccac ttacagacac tcatccgcca
    12181 gagcggattg ttcggttata gcctctatat tctgttattc attattgcca ctctctttct
    12241 gttaccagga agcatactgg tgatcgctgg aggaatagtt tttggcccgc tcttagggac
    12301 actactctca ttaattgccg ccacgctcgc ctcctcgtgc tcattcctgc tggcgcgctg
    12361 gctgagacgt gatttactgc tgaaatacgt tggtcatagc catacctttc aggccattga
    12421 aaaaggcatt gcgcgtaacg gtattgattt tcttattctg acccgcttaa tcccgttgtt
    12481 tccttacaat attcaaaatt acgcttacgg attaaccaca atcgccttct ggccttatac
    12541 ccttatttcg gcactcacga ccctgcccgg tattgttatt tataccgtga tggcaagcgt
    12601 tctcgccaat gaaggcatta cgctgcgctt tattttacaa ctctgtctgg cgggcctggc
    12661 gctgtttatt ctcgtccagc tcgcaaaact ctacgcccga cacaaacatg tggatctgtc
    12721 tgcttcgcgc cgcagcccac ttactcaccc aaaaaatgaa ggatagaacg atgtcgcaac
    12781 attattcagt gtcatggaaa aaaggactgg ctgcactctg cttactggct gttgccgggc
    12841 ttagcggctg cgatcaaaaa gagaatgcgg cggcaaaagt ggaatacgac ggactttcga
    12901 acagccaacc gctgcgcgtc gatgccaata accatacggt aaccatgctg gtgcaaatta
    12961 atggtcgttt cctcaccgac gacactcgtc acggtattgt gtttaaagat ggctccaacg
    13021 gacataaatc gctgtttatg gcttatgcga ccccgaaagc attttatgaa gccctgaaag
    13081 aggcaggtgg tacgccgggc gaaaacatga cgatggataa taaagaaacg actcatgtca
    13141 caggcagcaa actggatatt tcggtcaact ggcaaggggc ggcaaaagcg tattccttcg
    13201 atgaagtgat tgttgacagt aatggcaaga aactggacat gcgctttggc ggtaatttaa
    13261 cggcagcaga agagaagaaa acgggttgcc tggtgtgtct ggatagctgc ccggtcggca
    13321 tcgtcagcaa tgcaacatac acttatggtg cggtggaaaa acgcggcgaa gtgaaattca
    13381 aaggcaatgc ctcagttctc ccggcggata acacgctggc aacggttacc tttaaaatca
    13441 ccgaataaag ccaggataaa ggatgatgaa gatacaatcg cgtaaaatct ggtactaccg
    13501 tataaccctc atcatcctgt tgttcgctat gctgctcgca tgggcgctgc ttcccggcgt
    13561 ccatgagttt atcaatcgca gcgttgcggc gtttgccgcc gtggaccaac agggtataga
    13621 acgctttatt cagtcttacg gcgcactggc ggcagttgtc tcgttcttgt tgatgatttt
    13681 acaggccatt gctgcaccgc tacctgcgtt tttgatcacc tttgccaatg cgtcgctgtt
    13741 tggcgcgttc tggggcggct tgctgtcgtg gaccagttcg atggccggcg cggcgctgtg
    13801 cttttttatc gccagagtga tgggccgcga agtggtggaa aaattaaccg gcaaaaccgt
    13861 gcttgacagt atggacggct ttttcactcg ctacggcaaa cacaccatcc tggtctgtcg
    13921 gttattgcct tttgtccctt tcgatccaat cagctatgct gccggtctga cttcaatacg
    13981 ttttcgctcg ttttttatcg ccaccgggct tggtcaatta ccggcgacta ttgtttattc
    14041 ctgggcgggc agcatgttaa caggcggtac tttctggttt gtcaccggac tgtttattct
    14101 gtttgccctg accgtggtga tttttatggc gaagaaaata tggcttgagc gccagaagag
    14161 gaatgcctga tgggtttacc gccgcttagc aaaattcctt tcattttacg tccgcaggcg
    14221 tggctgcatc gtcgccatta cggcgaggtg ctaagcccga ttcgctggtg ggggcggatc
    14281 ccgtttatct tttatttggt gtcaatgttt gttgggtggc tggagcgcaa acgctcaccg
    14341 ctcgatccgg tagtgcgatc gcttgtcagc gcgcgcattg cgcaaatgtg cctgtgtgag
    14401 ttttgcgtgg atatcaccag tatgaaagtc gccgagcgta ccggcagcac cgataaactg
    14461 ctggcagtgg ctgactggcg gcaaagcccg ctcttcagcg atgaagaacg gctggcgctg
    14521 gagtacgccg aagccgcaag cgtaacgccg ccaacggtcg atgatgccct gcgcacccga
    14581 ctggctgcgc attttgacgc tcaggcgctc accgaactga cggcattgat cggcctgcaa
    14641 aacctgtcgg ctcgttttaa ttctgccatg gacattcccg ctcaggggct gtgccgtatt
    14701 ccggaaaaac gttcttaagg agagaagatg cgccattgtg ggtggttgct gggattgtta
    14761 tcgctgtttt ctctggcaac acatgccagt gactggcagg aaattaaaaa tgaggccaaa
    14821 gggcaaaccg tctggtttaa cgcctggggc ggcgataccg caattaaccg ctatctcgac
    14881 tgggttagcg gcgagatgaa aacccattac gctataaacc tgaagattgt tcgtctggcg
    14941 gatgccgcag acgcggtgaa gcgcattcag accgaagctg ctgccggacg taaaacgggc
    15001 ggctcggtgg atctgctctg ggtgaacggc gaaaacttcc gcaccttaaa agaggccaat
    15061 ttattacaaa cgggctgggc ggagactctg cccaactggc gctatgtcga cacacagctg
    15121 ccggtgcggg aagatttttc agtgcctaca gaaggggctg aatcgccctg gggcggcgca
    15181 caactgacgt ttatcgcccg ccgcgatgtt acgccacagc caccacaaac gccgcaagcc
    15241 ttactggagt ttgctaaagc caatcccggc acggttacct atccgcgccc accggacttt
    15301 accggcacgg cgtttcttga acagttgctg attatgctga cgcccgatcc cgccgcgtta
    15361 aaagttgccc cggataaagc cactttcgcc cgcgtcactg cgcccttgtg gcaatatctt
    15421 gacgcgctac atccctattt atggcgcaaa ggaaaggatt tcccgccttc gcccgcgcgg
    15481 atggatgctc tgctgaaagc cggcacattg cgcctgtcgc tgacctttaa ccctgctcat
    15541 gcccagcaaa aaatcgccag cggcgatttg cctgcaagca gttacagttt tggttttaac
    15601 caggggatga ttggcaacgt gcatttcgtc accattcctg ctaacgcaaa tgccagtgct
    15661 gcggcgaagg tggttgccaa cttcctgctc tcacccaatg cgcaactgcg taaagcggat
    15721 cccgctgtct ggggcgatcc ttctgttctc gatccgcaaa aactgcctga tgggcagcgc
    15781 gaaacattgc aatcaagaat gccgcaagat ctgccgccgg tactggctga accgcacgca
    15841 ggttgggtaa atgcgctgga acaagaatgg ctacaccgtt acggtacgca ttaatctttt
    15901 tgctgtgggc gatggtggcg gtgatttatg caccactgat cccggcagct ctcacgctga
    15961 tatcgcctgc tttgtcgttg acacactggc aggcgttatt tgccgatcca cagttaccgc
    16021 aggcattact ggcaacgctg gtgtcgacaa ccatcgcggc ggtcggggcg ttgttgattg
    16081 ccctgctggt gattgtggcg ctgtggcctt ggccgaaatg gcagcgtatg tgtgcccgtc
    16141 tgccgtggct gctcgccatt ccccatgtgg cttttgccac cagcgccctt ctgctctttg
    16201 ctgacggagg gctgctttat gactatttcc cgtatttcac tccgctaaag gacaaattgg
    16261 gtattgggct gggcctcacc cttgcagtga aagaaagcgc atttctgctg tggatcttag
    16321 cggcagtatt gagcgaaaaa cggctgttgc agcaggtcat tgtgctggat tcgctgggct
    16381 acagccgctg gcaatgcctg aactggctgc tcttgccctc cgtcgcacct gcactggcaa
    16441 tggcgatgtt ggcgattgtt gcctggtcgc tttcggtcgt ggatgtggcg attattcttg
    16501 ggccaggtaa tccaccgacg ctggcagtaa ttagctggca gtggttaact cagggcgacg
    16561 cggatcaaca aacgaaaggc gcactcgcca gtctgctgct gatgctgtta ctcgccgcct
    16621 acgttttgct gagctatctg ctatggcgca gctggcggcg cactattccc cgcgtagatg
    16681 gcattcgcaa gcctgccaca cctttattac caggcactac gctggcgagt tttttaccct
    16741 taaccggtgt gctgtgtgtg gttctgctgg cgatcctcgc ggatcagtcg acgatcaata
    16801 gtgaagcact catcaacagc ctgacaatgg ggctggtggc gacattcatc gctttgctcc
    16861 tgttactgct gtggctggaa tgggggccac agcgtcgcca gttgtggctc tggttgccca
    16921 ttctattgcc cgctctgcca ctggtggcgg gccagtacac gctggcgcta tggctgaacc
    16981 tggatggaag ctggacggcg gtggtctggg ggcatctgct gtgggtaatg ccgtggatgc
    17041 tgtttatcct gcaacccgcc tggcagcgca ttgattcacg gttaattttg attgcgcaaa
    17101 cactgagctg gtcgcgggcc aaaatcttct tttacgtgaa atgtccgctt atgctacgcc
    17161 ctacgctgat tgccttcgcg gtgggatttt cagtcagtat tgcgcagtat atgccgacgc
    17221 tgtggctggg cgcggggcgt tttccgacgc tcaccactga ggcggtggca ttaagcagcg
    17281 gcggcagcaa tggtattttc gccgcccagg ctttatggca actgctatta ccgcttatta
    17341 tttttgccct gaccgcgtta gtcgcaaaat gggtaggtta tgtcagacaa ggactccgct
    17401 aatgctctgc gtgaaaaatg tttcgctacg tttaccagaa agccgcttgc tgacaaacgt
    17461 taactttacg gtggataaag gtgacattgt cacgttaatg gggccgtctg gctgtggaaa
    17521 atccactctg ttttcatgga tgattggtgc actggctgga cagttttctt gtacaggtga
    17581 gctatggctc aatgagcaac ggattgacat gctacccacc gcacagcgtc agattggcat
    17641 tcttttccag gatgcactgt tatttgacca gttcagcgtc gggcaaaatt tactgctggc
    17701 gctaccgtcg acgctcaagg ggactgccag gcgcaatgcc gtgaaagacg cgcttgatcg
    17761 agcgggactg gctgaaactt atcaccagga ccctgcgaca ttatcaggcg gtcagcgagc
    17821 gcgggtggcc ctgctgcgcg cccttctcgc tcagccaaaa gcgttactcc tggatgagcc
    17881 attcagccgt cttgatgtgg ctctgcgcga taattttcgc cagtgggtgt tcagcgaagt
    17941 tcgcgaactg gcgatccccg tcgttcaggt aacgcacgat ctccaggatg ttcctgctga
    18001 tagttctgtt ctggatatgg cgcagtggtc agaaaattac aacaaactgc gataacgcaa
    18061 agtttttctc aatgcgtcag ttcagaatgg cgcactcaaa actacaatgt cgggattttc
    18121 gatgaaacgt gtttctcaaa tgaccgcgct ggcaatggct ttagggctgg cttgcgcttc
    18181 ttcgtgggcc gctgaactgg cgaagcctct tacacttgac cagcttcaac aacaaaatgg
    18241 caaagcgata gatactcgcc ccagcgcgtt ttataacggc tggccacaaa ccttaaatgg
    18301 cccttctggt catgaacctg ccgccttaaa cctctctgcc agctggctcg acaaaatgag
    18361 caccgaacag ctcaacgcgt ggatcaagca acataacctg aaagccgatg ccccggtggc
    18421 gctgtacggt aatgacaaag atgtcgacgc cgtcaaaacg cgactgcaaa aagcaggttt
    18481 tacgcatatc tccattctga gtgacgcgct aagcgaacct tcccgtctac aaaaactgcc
    18541 gcactttgag cagctggttt atccgcaatg gctgcacgac ctgcaacaag gtaaagaggt
    18601 tacggcgaaa cctgccggtg actggaaagt cattgaagcg gcctggggcg ctcctaagct
    18661 ttaccttatc agccatattc ccggtgctga ctacatcgat accaacgaag tggaaagcga
    18721 accgctgtgg aacaaagttt ctgatgaaca actaaaagcg atgctggcaa aacacggcat
    18781 tcgccatgac accacggtca ttctgtacgg gcgtgacgta tacgctggag cgcgtgtggc
    18841 gcaaattatg ctctacgcag gcgtgaaaga tgtgcgtctg ctggatggcg gctggcaaac
    18901 ctggtccgac gcgggactgc ctgttgagcg aggaacgcca ccgaaagtga aagcggaacc
    18961 ggatttcggc gtgaagatcc cggcacaacc gcagttgatg ctcgatatgg aacaggcccg
    19021 tggactgctg catcgccagg atgcatcgct ggtgagcatt cgttcgtggc cagaatttat
    19081 cggtacgacc agcggttaca gctatattaa accaaaaggt gaaatagccg gagcacgttg
    19141 gggacacgct ggtagcgact cgacgcatat ggaagatttt cataatccgg atggcaccat
    19201 gcgcagcgcc gatgatatta ccgctatgtg gaaagcgtgg aatatcaaac cagagcagca
    19261 agtttcattc tactgcggca ccggctggcg cgcgtccgaa acctttatgt acgcacgcgc
    19321 catgggttgg aagaatgttt ccgtgtatga cggcggctgg tacgaatgga gcagcgatcc
    19381 aaaaaatccg gtagcaaccg gtgaacgcgg cccggacagt agcaaataac attgcaatta
    19441 ctgacgctgg tgcgacttca gcgtcagata cccactccac acccgcgtaa aggtcgtcat
    19501 ccagcacaac gcaccgaaga tccacgcaaa ccacgcaaac catgcaggga ataaacatcc
    19561 cagcacaaac agtaagatcg tttctgtgcc ttcggttaac ccgcccagat aataaaacga
    19621 tttatgcgca taaccgggat tatcaatctg atgtttcgcc gccagcgcag caaaagcgag
    19681 aaaactgctg cctgtgccga taaacgcaaa cagcagccag ccgcctgcca gggcgttttg
    19741 ctgcggtgcg gcgaggataa acccaaaagg taccagcgcg taaaaaagaa aatcgagaga
    19801 aatatcaaga aagccgcccg cgtcggtaag ccctctgcgc cgcgccagcg caccgtccag
    19861 accatcaagc agcctgttca acaaaatgac gacaagcgcc gccagatacc agcccagcgc
    19921 cagaaaaggc agtgccagca cgccgatggc aaatcccact aatgttaaac catctggcgt
    19981 aatgcccggt ttatcaagaa cccgcacgca ctgatgcaac aacggtttaa tccggggatg
    20041 aagatggcgg tctagcacag gcactcctta aatataaagc ctttctgatt gagcaacagt
    20101 gcggatatta tggcattttt cgcttatctg cccgtgtgta atttatgaaa atgattgaag
    20161 ttgttgccgc catcattgaa cgtgatggca aaattttact cgcgcaacgc cccgcccaga
    20221 gcgatcaggc gggattatgg gagtttgccg gtggtaaagt cgagccggat gaaagccagc
    20281 ggcaggcgct ggtgcgtgag ttacgcgaag aactgggcat cgaagcaact gtgggtgagt
    20341 atgttaccag ccatcagcga gaagtttcgg gccggattat ccatcttcat gcctggcacg
    20401 tacccgactt ccacgggacg ttacaggcac atgaacatca ggcgctggtc tggtgctcac
    20461 ctgaagaggc gctgcaatat ccgctggccc ctgctgacat tccattatta gagtcgttta
    20521 tggctttacg cgccgccaga ccagcggatt agtgctaagg gttttgtcat cccgctggca
    20581 ttgcagcagt attccctcgg ctttaattac cgcgccttca gaataatttt tatcctgata
    20641 aacgcagcac tgagtacagg gctgcgctga ctgcccgcct gaactgaaca cttctggcgg
    20701 tacgtttacc tccacgtccg gacgataatg cgggttagcc agtgcaatta atggaaatgc
    20761 taatactacg gcgaacaatg ctcgactcac agggaactcc ttaacgttat tgtctctgct
    20821 actgataacg gtagccgggt ggcaaaactt tagcgtctga gttatcgcat ttggttatga
    20881 gattactctc gttattaatt tgctttcctg ggtcattttt ttcttgctta ccgtcacatt
    20941 cttgatggta tagtcgaaaa ctgcaaaagc acatcacata aacaacataa gcacaatcgt
    21001 attaatatat aagggtttta tatctatgga tcagacatat tctctggagt cattcctcaa
    21061 ccatgtccaa aagcgcgacc cgaatcaaac cgagttcgcg caagccgttc gtgaagtaat
    21121 gaccacactc tggccttttc ttgaacaaaa tccaaaatat cgccagatgt cattactgga
    21181 gcgtctggtt gaaccggagc gcgtgatcca gtttcgcgtg gtatgggttg atgatcgcaa
    21241 ccaggtacag gtcaaccgtg catggcgtgt gcagttcagc tcagccatcg gcccgtacaa
    21301 aggcggtatg cgcttccatc cgtcagttaa cctctccatt ctcaaattcc tcggcttcga
    21361 acaaaccttc aaaaatgccc tgactaccct gccgatgggc ggtggtaaag gcggtagcga
    21421 ttttgacccg aaaggtaaaa gcgaaggcga agtgatgcgt ttttgccagg cgctgatgac
    21481 cgagctttat cgtcatctgg gcgcggatac cgacgttccg gcaggtgata tcggcgttgg
    21541 tggtcgtgaa gtcggcttta tggcggggat gatgaaaaag ctctccaaca ataccgcctg
    21601 cgtcttcacc ggtaagggcc tttcatttgg cggtagtctt attcgcccgg aagctaccgg
    21661 ctacggtctg gtttatttca cagaagcaat gttaaaacgc cacggtatgg gttttgaagg
    21721 gatgcgcgtt tccgtttctg gctccggcaa cgtcgcccag tacgctatcg aaaaagcgat
    21781 ggaatttggt gctcgtgtga tcactgcgtc agactccagc ggcactgtag ttgatgaaag
    21841 cggattcacg aaagagaaac tggcacgtct tatcgaaatc aaagccagcc gcgatggtcg
    21901 agtggcagat tacgccaaag aatttggtct ggtctatctc gaaggccaac agccgtggtc
    21961 tgtaccggtt gatatcgccc tgccttgcgc cacccagaac gaactggatg ttgacgccgc
    22021 gcatcagctt atcgccaatg gcgttaaagc cgtcgccgaa ggggcaaata tgccgaccac
    22081 catcgaagcg actgaactgt tccagcaggc aggcgtactg tttgcaccgg gtaaagcggc
    22141 taatgctggc ggtgtagcaa cgtcaggcct tgaaatggcg caaaacgctg cgcgcctggg
    22201 ctggaaagcc gagaaagttg acgcacgttt gcatcacatc atgctggata tccaccatgc
    22261 ctgtgttgag catggtggtg aaggtgagca aaccaactac gtgcagggcg cgaacattgc
    22321 cagttttgtg aaggttgccg atgcgatgct ggcgcagggt gtgatttaag ttgtaaatgc
    22381 ctgatggcgc tacgcttatc aggcctacaa atgggcacaa ttcattgcag ttacgctcta
    22441 atgtaggccg ggcaagcgca gcgcccccgg caaaatttca ggcgtttatg agtatttaac
    22501 ggatgatgct ccccagggaa catttcttat gggccaacgg catttcttac tgtagtgctc
    22561 ccaaaactgc ttgtcgtaac gataacacgc ttcaagttca gcatccgtta actttctgcg
    22621 atagcagcag atatgccagt aaagaaatcc cattagacta tttttttgat aatcttcttc
    22681 gctttcgaac aactcgtgcg cctttcgaga agcaagcatt atataatgcc aggccagttc
    22741 ttcttcaatt gtcccgtttt gaaaagctgt gcttgatatc gagatcatcc atgataattc
    22801 cgccgcccat attagcttcg ccgaggattt accggagcga ttattagcgc aatcagagat
    22861 atagtctgag ggaaaaacag caaatttatt caacaaggcg ataacctgct ctggggcttc
    22921 ctccatgttt gctttaaagg tattggctcc atggtcgcca gaaagaaaat gctccattaa
    22981 ggcacaataa ctttcgctat cttcgatacc ccattgatcc tctaaagact cgcgtctttt
    23041 acttatgata tcgatcgagt caaagggaag cacatgatat tggaaggtat ctttgccagg
    23101 ttcaggcttt cgcggccaga actccagcgt ttcagaccat tgcttatgat agaaccgata
    23161 aggtgcgatc aattgtagcg cctgtaactt ctcgatactg agcggctcaa tacctttagc
    23221 ctgataataa tgcagttgtt ctttttttgc tttaaaaccg gcccgaacaa taagccccat
    23281 cataattaat agataaagaa aagagcaacc cgcagtaatc aggcctcttt cattcaaacc
    23341 gttggatgtt atcgctgcga atacaaacat tacagcgaca acacatgtta aatacccccc
    23401 ccacttacaa agcagcatgg ccttattttc tttaatcatc cgttcaaaat tactattaaa
    23461 tatttcccag ccattaaaag aatacttctc gctcccagga tggttttgta ataaaacttt
    23521 tttcatcaca ttccctgtta ttacatactt tagaggcgat aattttatcc atgcaaaaaa
    23581 aatatccaac gaattttctt gatccgctgg atatttcaga atatgactcg aatagcacga
    23641 aagattcgct cgcttacgct atcgccccgc ttccggcttc atctgctggc ggactttttt
    23701 tcgcactacg tttacgcggt gcagcctttt tcttatcagc actgccacca ctgcccggag
    23761 ccacaatgcc gcgaaactgc cgcaccggcg tacgtttggc ttgatcaata agttgatata
    23821 gcgtccccac cagcggctgc ataaagtcct gatagcgaca ctgcttttcg ctgatttgcg
    23881 tcagcaccga ttcccagtgc gcggtcatgt ccggtcgcgt cgccatttct ggcagcgaat
    23941 ggaataacgc ttttccggcg tcggtagagt ggatatagcg cccttttttg gtcagaaaac
    24001 cacgcttgaa cagcagttca ataatcccgg cacgggttgc ttctgtcccc agaccatcgg
    24061 tcgcacgaag gatctttttc agatctttgt cctgcacaaa gcgcgcaatc ccggtcatcg
    24121 ccgaaagcag tgttgcatcg gtaaaatggc gcggcggctg ggtttgccgc tctaccactt
    24181 cacctttttc acacagcaac tcatcgcctt tcgccaccac aggcaatggc gtgccgtcgt
    24241 tttcttcatc gcgctctttg ctgcctaaca gcgcgcgcca acctgcttca gcaagaaaac
    24301 gcgctttagc gacaaatttg cctttggcaa tgtccagttc gataacacac ttgcggaaca
    24361 ccgcatccgg gcagaactgc atcagatact gacgggcaat caggttatag accttcgctt
    24421 cgttctccgt caggttgatc gcagaactcc gtgcggtcgg aatgatggcg tggtgcgcat
    24481 cgaccttttt gtcatcccaa cagcggttgc gtatatctgg atctaccact ggctgcggca
    24541 acagatctgg tgcatgaacg ctgatggcat tcatcaccgc gtggcgtccg gcaaaatgtt
    24601 cttctggcaa atagcgacaa tcggaacgcg gataagtgat taacttgtgt gtttcgtaca
    24661 gcttctggca gatatcaagc acgttctgcg cgctcagacc aaagcgtttt gccgcttcaa
    24721 tctgcaacgc cgaaagcgaa aacggcagcg gcgcggattc tgattcccgt ttatcgttat
    24781 agctggtgac aatagccggt tgaccactaa tgcggttaac cacatgctcc gccagtggac
    24841 gatgtaacaa gcgcccttct tcatcctggt acggttcaca cgcttcgctc ggttgccaga
    24901 tagcggtaaa ccgctcatcg gcaggtgtca cgatatgcgc tttgacttca aagaagtctt
    24961 tcgccacgaa gttttcaatc tcttcatcgc ggcgcaccac cagcccgagc acgggtgtct
    25021 gcacgcgtcc cacggaaagt acgccctgat aaccggcatt gcgaccgaga atggtatacg
    25081 cacgggtcat attgatgccg tacagccagt cggcacgcgc tcgcgccagc gcagaaacgc
    25141 acaatggcac aaactcactg ttggaacgaa gacggtcgat cgcccgctca accgcctgcg
    25201 ggttcaggtc gtttatcaag caacgctgca cctgctggcg cttttccggt gccagttgca
    25261 gatagtccag cacttcatcc accagcaatt gcccttcacg atccgggtcc ccggcgtgaa
    25321 cgatttcgct ggcttcatgc aggaagcgtt tgatgacgtt aagttgtttg gtcacggagg
    25381 gtcggggctg taattgccac ttttccggga caatcggcaa atccgcaaga ttccagcgcg
    25441 catagcggct gtcgtaggcc tctggctgcg cctgctcaag caggtgaccg atacaccagg
    25501 tcaccacctg accattaccg cactcgataa agccatcgcc tttacggtgc ggtttgggca
    25561 ggacatcagc aatggcgcgc gccagactcg gtttttcggc aataaacaac cgcattgaat
    25621 taacggatct caaccatggc acgaccgccg cgcgctggca ccagttcgcc aatcgccgtc
    25681 agttcaatgc caaactcggc ggctgtagtt ttaacctcat tttctgcttc cggcatgacc
    25741 gccagcagca aaccgccaga agtttgcgga tcgcacagca gatcgcgcac ttcacgcggc
    25801 atttcaccca tcagatgacc gtagctggca aagttacgtt cggtgccgcc aggcactgcg
    25861 cccaacttaa tgtactcttc aacccccggc aattttggga tcgcgtcata gtcaacgcgc
    25921 gcctgcacac ctgcgccctg acacatttcg ctcaaatggc ccagcagacc aaaaccagta
    25981 acgtcggtca tcgcttttac gccttcgatg ttggcaaagg acgcacctgc gatgttcatc
    26041 cggcacatca cttccgtcgc cagtccctga tgttctggtt tcaacagtga ttttttctca
    26101 gccgtggtaa gaacgccgat ccccagcggt ttcgtcagga acagtttgca tccggcttgt
    26161 gcggtactgt ttttcttcac ccgctcggtc ggtacgatcc ccgttaccgc cagaccaaaa
    26221 atcggctccg gcgcatcgat ggagtgaccg ccagccagcg caatacccgc ctgacggcat
    26281 gcatagcgtc caccttcggt tacttcgcgg gcaatttctg gggaaagttt gttaatcggc
    26341 cagccgagga tcgcaatcgc cataatcggt ttgcccccca tcgcgaagat atcgctgatg
    26401 gcgttagtcg ccgcaatgcg gccaaaatcg aaaggattat caacgatcgg cataaagaag
    26461 tcggtggtac tgataacgct ggtgccattg cccagatcgt acaccgccgc atcgtcgcgg
    26521 gtctcattac ccacaagcaa attcggatca acaaacttcg cctgctcact gtgcaggatg
    26581 gtttccaaca cttttgggga aattttacag ccgcaaccag ctccgtggct gtattgggtc
    26641 aaacgaatcg agttctcgct catggacatc tcctgtcaat gcaatccggg tatggtaacc
    26701 ctcattccgt gaagtgataa gtgagagtgt ctgaattcct gcgcctttgc tcacaatcca
    26761 gacagtttcg cgacaattat cagaaataag ttacaaacgg cgtcgggtcc gggacgttaa
    26821 tcgacgtaga tgctttcaac tgcggcgtgc cgaggtagag aaaaccgaca attttatcct
    26881 gctcacggca accgaatgct tcacgcacta ccggactttc agttaatgcg ccactgcgcc
    26941 agatgccgcc aaatccttgg gcaacggctg ccatttgcat cgccatgacc gcgcatccgg
    27001 cagacatttc ctgttcccat cgcgggactt tatgattttc ttcgcatttc gccaccaccg
    27061 tgatgatgag cggtgcgcgg aacggcgcat tacgggcttt gccgatagct ttgtcatcac
    27121 taccggcagc aatcgccccc tgttccagta cggcgctgaa acgctcgcgc ccttcccctt
    27181 caatcacaaa aaaatgccac ggttgcatgg acttatggtc cggcgcacgc atacccgcac
    27241 gcaggatgtt ttgcagttgt tcacccgttg gcgcgggttc agccaggcgg gaggcgctac
    27301 ggcgattgat caatagttcg agtgcatcca tttgattaac tcctgtcgtg atatttattc
    27361 acaaaattaa cacgagagtg gattttgtta cagcacagtc cgcaattcct gctgacaagt
    27421 gccggttggg tcattacgat aaccacatct attgcgcctg tgacaggtgt gaccttaagt
    27481 tgggagaata catgcgaacc ctttggcgat ttattgccgg attttttaaa tggacgtggc
    27541 gtctgctgaa tttcgtccgt gaaatggtac ttaacctgtt ctttattttc ctcgtactgg
    27601 ttggtgtggg gatctggatg caggtcagtg gtggtgattc gaaagaaacg gccagtcgtg
    27661 gcgcactgct gctggacatt tctggtgtga tcgtcgataa acccgacagt tctcagcggt
    27721 ttagtaaatt aagccgccag ctgcttggtg ccagttccga tcgtctgcag gaaaactcac
    27781 tgtttgatat cgtcaacacc attcgccagg cgaaggacga ccgcaatatc accggtatcg
    27841 tgatggatct gaaaaacttc gcaggcggcg accagccgtc tatgcagtac atcggcaaag
    27901 ccctgaaaga gtttcgtgac agcgggaaac cggtttatgc cgttggcgag aactacagcc
    27961 aggggcaata ttatctcgcc agtttcgcca ataaaatttg gctgtcaccg caaggcgtgg
    28021 ttgatctgca cggttttgct accaacggtc tgtactacaa atcgttgctg gataagctga
    28081 aagtttccac ccatgtgttc cgcgtgggta cgtataaatc tgccgttgaa ccgtttattc
    28141 gtgatgatat gtcaccggca gcccgcgaag ctgacagccg ctggattggt gagctgtggc
    28201 aaaactatct gaatactgtt gccgctaacc ggcagatccc tgctcagcag gtattccctg
    28261 gcgcgcaagg gttgcttgag ggtttaacca aaaccggtgg cgataccgcg aaatatgcac
    28321 tggaaaacaa gctggtcgat gcactggcat cgagtgccga aatcgaaaaa gctctgacca
    28381 aagagttcgg ctggagtaag actgataaaa attatcgcgc catcagttat tacgattacg
    28441 cattgaaaac gccggcagat accggtgaca gcatcggtgt cgtcttcgct aatggcgcaa
    28501 ttatggatgg cgaggaaact caggggaatg ttggcggtga taccactgcg gcacaaatcc
    28561 gcgacgctcg ccttgacccg aaagtgaaag cgattgtcct gcgtgttaat agcccaggcg
    28621 gcagcgttac cgcgtctgaa gtgattcgcg ctgaactggc agcagcccgg gcagcgggta
    28681 agcctgtggt tgtatcgatg ggcggcatgg cggcatctgg tggttactgg atttccacgc
    28741 cagctaatta cattgtggct aaccccagca ccctgaccgg ttctatcggt atcttcggcg
    28801 tgatcaccac cgtagaaaat agtctggatt cgattggtgt tcatactgat ggtgtctcaa
    28861 cttcaccgct ggcggatgtt tctatcacca gggcactgcc gccggaagcg cagcagatga
    28921 tgcaattaag cattgagaat ggctataaac gctttatcac gctggttgct gatgcgcgtc
    28981 attcgacgcc ggagcaaatt gataaaatcg cccagggcca cgtctggacc ggtcaggatg
    29041 caaaagctaa cgggctggtc gatagtctcg gggatttcga tgatgcggtt gccaaagcag
    29101 cagagctggc aaaagtgaaa cagtggcatc tggaatacta cgttgatgaa ccgaccttct
    29161 tcgacaaagt gatggacaac atgtctggtt ctgtccgggc aatgttgcca gatgcgttcc
    29221 aggccatgtt acctgcaccg cttgcctcgg tagcctctac cgttaaaagt gaaagcgaca
    29281 agctggccgc gtttaacgac ccacaaaacc gttatgcgtt ttgcctgacc tgcgccaacg
    29341 tgcgttaagt cttgtactga gtggccgaca gatcgtcggc cacattattt tttacctcga
    29401 cgaatcctct tcccgctgtc tcgacccata tttccttatc cgcatagtat caggtgcgct
    29461 cctcctgcct cacgtatata cttttgctct ttcgatatca ttcatatcaa tatcatgcaa
    29521 aagaaatcaa tttacgtcgc ctacacgggc gggaccatcg ggatgcagcg ttccgagcag
    29581 ggttatattc cggtgtcagg tcatctacaa cgccaactgg cgctgatgcc ggaattccat
    29641 cgcccggaga tgccagattt caccattcat gaatatacgc cgttgatgga ttcttctgat
    29701 atgacgccgg aagactggca gcatattgct gaagatatta aagcgcacta tgacgactat
    29761 gatggttttg tcattctaca cggtaccgac acgatggcgt ataccgcctc tgcgctgtcg
    29821 ttcatgctcg agaatctcgg taaaccggtc attgtgacag ggtcacaaat cccgctggct
    29881 gagttacgct ctgacggaca aattaatctg ctgaatgcgt tgtacgttgc ggcgaattat
    29941 ccgatcaacg aagtaacgct ctttttcaat aaccgattgt atcgcggcaa ccgcactacc
    30001 aaagcccatg ccgatggttt tgatgcgttt gcctctccaa accttcctcc gttactggaa
    30061 gctggtatcc atattcgtcg tttgaatacg ccacccgccc cgcacggtga aggggaatta
    30121 atcgttcatc caatcacgcc acaaccaatt ggcgtagtga cgatttatcc ggggatttct
    30181 gctgatgtcg tgcgcaattt tctgcgccaa ccggtgaaag cattgattct gcgctcctat
    30241 ggcgtgggta atgcgccaca aaacaaagcc ttcctgcagg aattacaaga agccagcgat
    30301 cgcggtattg tggtggtcaa cctgacacaa tgtatgtccg gtaaagtgaa tatgggtggt
    30361 tatgccaccg gtaacgccct cgcccatgcc ggcgttattg gcggtgcaga tatgactgta
    30421 gaagccacgc taaccaaact gcattacctg ctgagccagg aactggatac tgaaaccatt
    30481 cgcaaggcca tgagccaaaa cctgcgcggc gaactgacgc cggatgatta aggagactgc
    30541 aatgccccat cgcgccctgt tactggtcga tttacaaaat gatttctgtg ctggtggcgc
    30601 gctcgccgta ccggaaggtg acagtacggt ggaggttgct aaccgcctta ttgactggtg
    30661 ccagtcgcgc ggagaagcgg ttatcgccag ccaggactgg catccggcga atcacggcag
    30721 ttttgccagt cagcacggtg tagagcctta tacgccaggc caactcgacg gtttgccaca
    30781 aaccttctgg ccagatcact gtgtgcagaa cagtgaaggc gcacaattac atccgttact
    30841 gaaccaaaaa gcgatcgcag cggtgttcca taaaggcgaa aatcctttag ttgacagtta
    30901 cagtgccttt tttgataacg gccgtcggca gaaaacctct ctcgatgact ggttacgcga
    30961 tcatgaaatc gatgaattga tcgttatggg cctggctact gactattgcg tgaagtttac
    31021 cgtgctggac gcgttacagt taggttataa ggtgaacgtg attaccgatg gttgtcgtgg
    31081 cgtgaatatc cagccccagg acagtgcgca cgcgtttatg gagatgtcag cagctggggc
    31141 aacgctatat acgctggcag actgggaaga gacgcagggg taatattagt tgaattcatc
    31201 tctggcctac atttcctcaa tgacattgta ggccagataa tacttgtcag tgttgcattt
    31261 aacctacttg cagctaaaac acgacgtcct aacaagtata tttgccctcc cttaataaca
    31321 ggcttaaata ctagataact cattcactta aacatagcaa taggctttgc tgagatacca
    31381 aaatcttctt ttaactgctg tttgctcttc atgaccatct gaccatttgt gtcaattgtc
    31441 atatgctgtg cttcggtatt atggcgggct tgccacagca tcaccagttg caggcaattc
    31501 tctttttgtt cctcggtcaa cgcaacgcca tcaggccatt tccccagttc aacggcggtc
    31561 gacaaacgct ggtatacctc aggcgtcatg ctgttgataa tgtcatcaag attcataatt
    31621 tttccgctcc tgtggaataa gttgctgaat cgttttttca accgttgatt tcttcgccgt
    31681 tttcgccatc agtgaagcgt aaagaagcag agttaacaca ataacgttcg cctgttggtt
    31741 gcggcccgtc gggaaagaca tgccccagat gggcatcaca gttaccgcaa cgaatttcta
    31801 tgcgctgcat tccatgtgac aagtctttga tataacgaat ggattcttca ctcaccggtt
    31861 cgtagaaact gggccagcca cagccggaat catacttggt ttcggaatga aacagcgggg
    31921 catcgcagat caaacagtga tatacgccgt cacgcttgtt atgcagtaaa cgacccgtaa
    31981 atggcggttc tgtcccatga ttctgcgtca cgtaaaactg catctcggac aaattttttt
    32041 tcagttcttc tgccgaaggt ttattagcca tttgctcaca tctcacttta atcgtgctca
    32101 cattacgtga ctgattctaa caaaacatta acaccaactg gcaaaatttt gtcctaaact
    32161 tgatctcgac gaaatggctg cacctaaatc gtgatgaaaa tcacattttt atcgtaattg
    32221 ccctttaaaa ttcggggcgc cgaccccatg tggtctcaag cccaaaggaa gagtgaggcg
    32281 agtcagtcgc gtaatgctta ggcacaggat tgatttgtcg caatgattga cacgattccg
    32341 cttgacgctg cgtaaggttt ttgtaatttt acaggcaacc ttttattcac taacaaatag
    32401 ctggtggaat atatgactat caaagtaggt atcaacggtt ttggccgtat cggtcgcatt
    32461 gttttccgtg ctgctcagaa acgttctgac atcgagatcg ttgcaatcaa cgacctgtta
    32521 gacgctgatt acatggcata catgctgaaa tatgactcca ctcacggccg tttcgacggt
    32581 accgttgaag tgaaagacgg tcatctgatc gttaacggta aaaaaatccg tgttaccgct
    32641 gaacgtgatc cggctaacct gaaatgggac gaagttggtg ttgacgttgt cgctgaagca
    32701 actggtctgt tcctgactga cgaaactgct cgtaaacaca tcaccgctgg tgcgaagaaa
    32761 gtggttatga ctggtccgtc taaagacaac actccgatgt tcgttaaagg cgctaacttc
    32821 gacaaatatg ctggccagga catcgtttcc aacgcttcct gcaccaccaa ctgcctggct
    32881 ccgctggcta aagttatcaa cgataacttc ggcatcatcg aaggtctgat gaccaccgtt
    32941 cacgctacta ccgctactca gaaaaccgtt gatggcccgt ctcacaaaga ctggcgcggc
    33001 ggccgcggcg cttcccagaa catcattccg tcctctaccg gtgctgctaa agctgtaggt
    33061 aaagtactgc cagaactgaa tggcaaactg actggtatgg cgttccgcgt tccgaccccg
    33121 aacgtatccg tagttgacct gaccgttcgt ctggaaaaag ctgcaactta cgagcagatc
    33181 aaagctgccg ttaaagctgc tgctgaaggc gaaatgaaag gcgttctggg ctacaccgaa
    33241 gatgacgtag tatctaccga tttcaacggc gaagtttgca cttccgtgtt cgatgctaaa
    33301 gctggtatcg ctctgaacga caacttcgtg aaactggtat cctggtacga caacgaaacc
    33361 ggttactcca acaaagttct ggacctgatc gctcacatct ccaaataagt tgagatgaca
    33421 ctgtgatcta aaaagagcga cttcggtcgc tctttttttt acctgataaa atgaagttaa
    33481 aggactgcgt catgattaag aaaatttttg cccttccggt catcgaacaa atctcccctg
    33541 tcctctcccg tcgtaaactg gatgaactgg acctcattgt ggtcgatcat ccccaggtaa
    33601 aagcctcttt tgcattacag ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag
    33661 aagttctgtg gttgagcaac aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg
    33721 taccggtttg ctggccgtgg tttggtccgg cggcacaaca aggcctgcct gcgcacggtt
    33781 ttgcccgcaa cctgccgtgg acgctgaaat cacatcgtga agatgctgat ggcgtagcgc
    33841 tgacttttga attgacacaa agcgaagaga cgaaaaaatt ctggccgcac gactttacgc
    33901 tgttagcgca tttccgcgtg ggtaaaacct gtgaaatcga tcttgaagca cacggcgaat
    33961 ttgaaaccac ctctgctctg catacctact ttaacgtggg tgatatcgct aaggtaagcg
    34021 tcagtgggct gggcgatcgc ttcattgata aagtgaatga cgcgaaagaa gatgtactga
    34081 ccgatggtat tcagaccttc cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt
    34141 gcagcgtgat taatgatgaa gcgctgaatc gtattatcgc cgtaggccac cagcatcatc
    34201 tgaacgttgt cggctggaac ccgggaccgg cgctttcagt tagcatgggc gacatgccgg
    34261 acgatggcta caagaccttt gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag
    34321 tcaccaaaga gaaaccagca catctggcgc aatccattcg cgttgccaaa cgttaatttg
    34381 cgttaatgtt gtgtgccggg tgcgatgcat ccggcacgta tcaacatcac accatatcca
    34441 gcgcagtttt tccttttggt gccggatatg ccttatccag catagctaat tccgctgacg
    34501 aaagttcgac ctcaagcgca gctgcatttt gttggacatg ggcaatcgtg gccgcttttg
    34561 gaatcgccat cacaccatga tgactgatca cccacgccaa caatacttgt gccgcgctga
    34621 tattgtgagc atgtgcaatt tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc
    34681 gcccggcctg ggctaatgga ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg
    34741 gaaggagatc gtactcaatc cctcgtgaac cgaggtggta aagcacctga ttagtggcac
    34801 actgatttcc ccccggcagc tgccagagtt cctgcatatc agcataatca aggttagaaa
    34861 cgccccagcg gcggattttt ccctgggcga tcaatttttc catcgctgca acagtctctt
    34921 caaaagcgaa actgccagac cagtgtaata agtagagatc gagataatca gtattgagac
    34981 ggcgtaaact ggcttcgcat gcatttatcg ctttttgccc gccagcattc cacggataga
    35041 ctttagagac gagaaagacc ttctctcgca gactggttaa tgcttcccca accacctttt
    35101 cggcaccgcc atcggcatac atttcggcgg tatcaatgag ggttaaaccg agctcaatgc
    35161 ccgcgcgtag tgcagcaact tctgttttgc gctgactggc atcttcgccc atataccatg
    35221 ttccctgccc tatggctggc agtgagacat cgccactaaa ttgaatcatt ttttgttgca
    35281 ttgtttcctc ccaggtattg caccaccgta atgcaaaaca gggcgtgacg ccctgttttt
    35341 atgcacaaaa tgccctggaa agatgcatta tcagaatttg taggtgatcc cggtagaaat
    35401 caggccagtc caggatttat ccaccatcgg gctgtcagta acttcatcag acagacgggt
    35461 atagcgcgcg gtaccgtaaa cactccagtc gccgaggaag ttgtagctgg cgctcagctc
    35521 caggtaaggg ctccagctgt cgttcggatt atagccacgc agaccgctgc gagcggactc
    35581 tttgcgcgat acgccataat agtattcgtt ctggttttcg ctgttccact gcacaccgat
    35641 acccggggtc acggtcaggc cgccgttggt gtaacgatac aaccaggcca tatcccagac
    35701 aatgccgttg ctgttatcca gggtatcgcc agccagggtg gtacgcaggt aaccgtactg
    35761 ggtaaagtga gcataagaca gaccagccat catggtgctc ttacggtcat ccaggtgacg
    35821 catttggtga tcgccactgt ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat
    35881 tgaaagtttg tccgttgcgt cattccacag gtagtaacca ccacctaagc cacggaacca
    35941 gaagttatcg ccttcatagt tgattaccgg tactgggtaa acatcggtat cgtaatcttt
    36001 atatgggtgt tcaacgacac ctacgcctgc gcccagggaa aatttacctt cagcgtgcgc
    36061 tacgcctgca gacgttgcga taagcactcc aagtgccaga agtttgagtt tggtcacaat
    36121 taatcattcc ttaaacaaat gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa
    36181 cccaaccttt ttacgttttc attttttaaa gtaactgttt aattttcctg acgcggatga
    36241 caccgcgctt acagccaaat gaatttagcg ttactggcga gcctggtctt tacattaatt
    36301 atgcaaaatt tatggatgag ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca
    36361 agttttccat ttgccatcta cgcttaattt tgaaggtgta tcaccgggca cgttgttctc
    36421 atcgtcgata aaatggcatg agagttgctg tgttttagca agagacgtcg ttcagtttac
    36481 ctcttccggg agcctctact attcatatga acggctctta acctgtgcta aaaaacgaaa
    36541 ggacggcata ccatgaatat attcgatcac tatcgccagc gatatgaagc tgccaaggac
    36601 gaagagttca cactgcagga gtttcttacc acttgtcggc aagatcgcag tgcttatgcc
    36661 aacgcggctg agcggctatt gatggctatc ggtgagcctg tcatggtcga tacagcccag
    36721 gaacccagac tttctcgact cttttctaac cgggtcattg cacgttatcc ggcgtttgaa
    36781 gagttttacg gcatggaaga cgcgattgaa cagattgtct cttatctgaa acacgcggct
    36841 caggggctgg aagagaagaa acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa
    36901 tcatcgcttg ctgagcgact gaaatcatta atgcagctcg taccgattta tgtattgagc
    36961 gccaacggtg agcgtagccc ggtcaacgat catccgttct gtcttttcaa tccgcaggaa
    37021 gatgctcaga ttctggaaaa agagtatggc attcctcgcc gttatctcgg caccatcatg
    37081 tcgccgtggg cggcaaaacg cctgcatgaa tttggtggcg atatcactaa gttccgggta
    37141 gtgaaggtct ggccgtcaat tctgcaacaa attgctatcg ccaaaacgga acccggtgat
    37201 gagaacaacc aggacatctc cgcgctggtt gggaaagtcg atattcgtaa actcgaacac
    37261 tacgcgcaga atgacccgga cgcctacggc tattccggtg cgctgtgccg cgccaaccaa
    37321 gggatcatgg aatttgtcga gatgtttaaa gcaccgataa aagtgctgca tcccttgtta
    37381 accgccaccc aggaaggtaa ctacaacggg acggaaggta tctccgccct gccgttcaac
    37441 gggattatcc tcgcccactc gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac
    37501 aacgaagcct tcctcgaccg tgtttacatc gtgaaggtac cgtattgctt gcgcatttcc
    37561 gaagagatca aaatctacga gaaattgctt aatcacagtg aattgactca cgccccatgc
    37621 gcccctggca cgctggaaac actgtcacgt ttttccattc tttcgcgcct gaaagagcca
    37681 gaaaactcca gcatttattc aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc
    37741 gatcccaaag ccaagtcgta tcaggaatat cgtgactacg ccggtgtcga tgaagggatg
    37801 aacggtctgt cgacgcgttt tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat
    37861 gtagaagtgg cggcaaaccc ggtccatctg ttctacgtcc tggaacagca gatcgagcgc
    37921 gagcagttcc cacaagagca ggcagaacgc tatctggagt tcctgaaagg ttatctgatc
    37981 ccgaaatatg ccgagtttat cggcaaagag atccagacgg cctaccttga atcctattcc
    38041 gaatatgggc aaaacatttt cgaccgttat gttacctacg cggatttctg gattcaggat
    38101 caggagtatc gcgatccgga taccgggcag ctgtttgacc gcgagtctct taacgccgag
    38161 ctggagaaaa tcgagaaacc ggcggggatc agtaatccaa aagatttccg caacgagatt
    38221 gttaacttcg tactgcgcgc cagagcgaat aacagcggac gcaatccgaa ctggaccagc
    38281 tatgaaaaac tgcgaacggt tatcgagaag aaaatgttct ccaataccga ggagctgttg
    38341 ccggttatct cgtttaacgc caaaacgtca accgacgagc agaagaaaca cgacgacttt
    38401 gtcgaccgta tgatggaaaa aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg
    38461 tatttgcgcg tacgtaaatc gtcttaacaa ccctggcccg gtacacatgc taccgggcct
    38521 acaacgacag cgaaccgtgg gccagagaaa tggcaacaca ggcgtagcat acagttggca
    38581 aatgtagtac ggggggcata tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa
    38641 aagcatggtg aatcgccagc gttttttacg ccgttataaa gcgcaaatta aacagtcgat
    38701 ctccgaggcc attaataagc gttcggtgac tgacgtcgac agcggcgagt ccgtatccat
    38761 tcccacggaa gatattagcg aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg
    38821 cgtgcatccg ggcaatgacc atttcgttca gaacgaccga attgaacgtc cccagggtgg
    38881 cggcggaggt tccggcagtg gtcagggcca ggccagccag gatggtgaag gtcaggatga
    38941 atttgtcttt cagatttcga aagatgagta tcttgatttg ctctttgaag atttggcctt
    39001 accgaatctg aaacaaaacc aacaacgcca gctgaccgaa tataaaacgc atcgggcggg
    39061 ttataccgct aacggcgttc cggcaaatat cagcgttgtg cgctccttgc agaactcact
    39121 ggcgcgacgc acagccatga cggcaggcaa gcggcgggaa cttcatgcac tggaagagaa
    39181 tttggccatc atcagcaaca gtgaacctgc gcagctgctg gaagaggaac gtctgcgcaa
    39241 agaaattgca gaattacgtg ccaaaattga acgcgtccct tttattgaca ccttcgattt
    39301 acgttacaag aactacgaga agcggcccga tccctccagc caggcagtga tgttttgcct
    39361 gatggacgtt tccggttcaa tggatcaatc cactaaagat atggctaagc gtttttatat
    39421 tctgctgtat ctgttcctca gcagaacgta taagaacgtg gaagtcgtat acatccgcca
    39481 tcatacccag gcgaaagaag tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg
    39541 caccattgtt tccagcgccc tgaaactgat ggatgaggta gtgaaagagc gttataaccc
    39601 ggcacagtgg aatatttacg ctgcacaagc atcggacggc gataactggg ccgatgactc
    39661 tccgctttgc catgaaatcc tggcgaaaaa attattacct gttgttcgtt attacagcta
    39721 tatcgaaatt acccgtcgtg cacatcagac attgtggcga gaatatgagc atctgcaatc
    39781 tactttcgac aactttgcga tgcagcacat ccgcgaccag gatgatattt atccggtgtt
    39841 ccgtgaactg tttcataaac aaaatgcaac agctaaag
//
LOCUS       DAFNBJ010000023        38413 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000023 DAFNBJ010000000
VERSION     DAFNBJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.023"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            124..2367
                     /gene="pdeF"
                     /locus_tag="L2X49_001210"
     CDS             124..2367
                     /gene="pdeF"
                     /locus_tag="L2X49_001210"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBN4796324.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIAFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTIAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2406..3947)
                     /gene="ppx"
                     /locus_tag="L2X49_001211"
     CDS             complement(2406..3947)
                     /gene="ppx"
                     /locus_tag="L2X49_001211"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBN4796325.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3952..6018)
                     /gene="ppk1"
                     /locus_tag="L2X49_001212"
     CDS             complement(3952..6018)
                     /gene="ppk1"
                     /locus_tag="L2X49_001212"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBN4796326.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6189..6827)
                     /gene="purN"
                     /locus_tag="L2X49_001213"
     CDS             complement(6189..6827)
                     /gene="purN"
                     /locus_tag="L2X49_001213"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBN4796327.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6827..7864)
                     /gene="purM"
                     /locus_tag="L2X49_001214"
     CDS             complement(6827..7864)
                     /gene="purM"
                     /locus_tag="L2X49_001214"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBN4796328.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8189..8815
                     /gene="upp"
                     /locus_tag="L2X49_001215"
     CDS             8189..8815
                     /gene="upp"
                     /locus_tag="L2X49_001215"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBN4796329.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8901..10190
                     /gene="uraA"
                     /locus_tag="L2X49_001216"
     CDS             8901..10190
                     /gene="uraA"
                     /locus_tag="L2X49_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBN4796330.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10240..10986
                     /gene="hda"
                     /locus_tag="L2X49_001217"
     CDS             10240..10986
                     /gene="hda"
                     /locus_tag="L2X49_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBN4796331.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11124..11483)
                     /gene="arsC"
                     /locus_tag="L2X49_001218"
     CDS             complement(11124..11483)
                     /gene="arsC"
                     /locus_tag="L2X49_001218"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBN4796332.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVESEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11504..12967)
                     /gene="bepA"
                     /locus_tag="L2X49_001219"
     CDS             complement(11504..12967)
                     /gene="bepA"
                     /locus_tag="L2X49_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBN4796333.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13180..14241
                     /locus_tag="L2X49_001220"
     CDS             13180..14241
                     /locus_tag="L2X49_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBN4796334.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14279..15127)
                     /locus_tag="L2X49_001221"
     CDS             complement(14279..15127)
                     /locus_tag="L2X49_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HBN4796335.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPTLTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15149..17161)
                     /gene="hyfR"
                     /locus_tag="L2X49_001222"
     CDS             complement(15149..17161)
                     /gene="hyfR"
                     /locus_tag="L2X49_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBN4796336.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17191..17604)
                     /gene="hyfJ"
                     /locus_tag="L2X49_001223"
     CDS             complement(17191..17604)
                     /gene="hyfJ"
                     /locus_tag="L2X49_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBN4796337.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17597..18355)
                     /gene="hyfI"
                     /locus_tag="L2X49_001224"
     CDS             complement(17597..18355)
                     /gene="hyfI"
                     /locus_tag="L2X49_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBN4796338.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLREADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(18352..18897)
                     /gene="hyfH"
                     /locus_tag="L2X49_001225"
     CDS             complement(18352..18897)
                     /gene="hyfH"
                     /locus_tag="L2X49_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBN4796339.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18907..20574)
                     /gene="hyfG"
                     /locus_tag="L2X49_001226"
     CDS             complement(18907..20574)
                     /gene="hyfG"
                     /locus_tag="L2X49_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HBN4796340.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAELDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(20564..22144)
                     /gene="hyfF"
                     /locus_tag="L2X49_001227"
     CDS             complement(20564..22144)
                     /gene="hyfF"
                     /locus_tag="L2X49_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBN4796341.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLEPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22149..22799)
                     /gene="hyfE"
                     /locus_tag="L2X49_001228"
     CDS             complement(22149..22799)
                     /gene="hyfE"
                     /locus_tag="L2X49_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBN4796342.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILQQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIAIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22811..24042)
                     /locus_tag="L2X49_001229"
                     /pseudo
     CDS             complement(22811..24042)
                     /locus_tag="L2X49_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(24059..25006)
                     /gene="hyfC"
                     /locus_tag="L2X49_001230"
     CDS             complement(24059..25006)
                     /gene="hyfC"
                     /locus_tag="L2X49_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBN4796343.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25017..27034)
                     /gene="hyfB"
                     /locus_tag="L2X49_001231"
                     /pseudo
     CDS             complement(25017..27034)
                     /gene="hyfB"
                     /locus_tag="L2X49_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27034..27651)
                     /gene="hyfA"
                     /locus_tag="L2X49_001232"
     CDS             complement(27034..27651)
                     /gene="hyfA"
                     /locus_tag="L2X49_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBN4796344.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDCLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(27904..28374)
                     /gene="bcp"
                     /locus_tag="L2X49_001233"
     CDS             complement(27904..28374)
                     /gene="bcp"
                     /locus_tag="L2X49_001233"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBN4796345.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28374..28946)
                     /locus_tag="L2X49_001234"
     CDS             complement(28374..28946)
                     /locus_tag="L2X49_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBN4796346.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29092..29970
                     /gene="dapA"
                     /locus_tag="L2X49_001235"
     CDS             29092..29970
                     /gene="dapA"
                     /locus_tag="L2X49_001235"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBN4796347.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            29987..31021
                     /gene="bamC"
                     /locus_tag="L2X49_001236"
     CDS             29987..31021
                     /gene="bamC"
                     /locus_tag="L2X49_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBN4796348.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31234..31947
                     /gene="purC"
                     /locus_tag="L2X49_001237"
     CDS             31234..31947
                     /gene="purC"
                     /locus_tag="L2X49_001237"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBN4796349.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32101..32328
                     /gene="vapB"
                     /locus_tag="L2X49_001238"
     CDS             32101..32328
                     /gene="vapB"
                     /locus_tag="L2X49_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBN4796350.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32328..32726
                     /gene="vapC"
                     /locus_tag="L2X49_001239"
     CDS             32328..32726
                     /gene="vapC"
                     /locus_tag="L2X49_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBN4796351.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            32873..33736
                     /locus_tag="L2X49_001240"
     CDS             32873..33736
                     /locus_tag="L2X49_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBN4796352.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33751..35766
                     /gene="tmcA"
                     /locus_tag="L2X49_001241"
     CDS             33751..35766
                     /gene="tmcA"
                     /locus_tag="L2X49_001241"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBN4796353.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHHFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLMRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35840..36538
                     /gene="ypfH"
                     /locus_tag="L2X49_001242"
     CDS             35840..36538
                     /gene="ypfH"
                     /locus_tag="L2X49_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBN4796354.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMVLESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36648..36848)
                     /locus_tag="L2X49_001243"
     CDS             complement(36648..36848)
                     /locus_tag="L2X49_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBN4796355.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36876..38003)
                     /gene="dapE"
                     /locus_tag="L2X49_001244"
     CDS             complement(36876..38003)
                     /gene="dapE"
                     /locus_tag="L2X49_001244"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBN4796356.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38007..38363)
                     /locus_tag="L2X49_001245"
     CDS             complement(38007..38363)
                     /locus_tag="L2X49_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBN4796357.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9333 a         9914 c        10105 g         9061 t
ORIGIN      
        1 aactttcata tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa
       61 gtcaggtggg atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca
      121 tctatgaaac tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg
      181 ttcctgcctt ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct
      241 tccggtgagg tttttctctt ttatctgcct ttggcactga tgatcagcat gatgatgttt
      301 ttcagctggg cggcattgcc agggatcacc ttagggattt ttgtccgcaa atatgcagag
      361 ctgggttttt acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt
      421 tggggcggtt acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt
      481 ttaatttccc agcgtatatt ctggcagatt gcgtttcctg caacgctgtt tctgatactt
      541 ttccagtttg ctgcatttgt aggattgctg gcgagcagag aaaatctggt cggtgtcatg
      601 ccttttaacc tcgggacttt aatcaattat caggccttac tggtgggtaa tctgatcggt
      661 gtcccgctgt gctacttcat cattcgggta gtgcgaaatc cattttattt acgtagctat
      721 tattcgcaat taaaacagca ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg
      781 ctactggcat taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcaca
      841 atttttagca ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcgatg
      901 cgctatggtt ataagctgat ttcgctgctc tgggcggtcg tgttgatgat cagcatccac
      961 agctatcaaa attacattcc catttatcct ggctatacca cgcagctgac cataacctcc
     1021 tccagttatc tggtattctc ttttattgtc aattatatgg ctgtactggc aactcgtcag
     1081 cgagcggtag tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca
     1141 aatgttcgcg ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta
     1201 cgcatccctg gcatggaaat gctggttaag aactatggca tcatgctgcg gattcaatac
     1261 aagcaaaaac tttctcactg gctgtcaccc ttgctggaac cgggtgaaga tgtttatcag
     1321 ctttcgggta acgatctcgc gctgcggctg aatacagaat cgcaccagga gcgcattacc
     1381 gcactggata gccatctcaa gcaatttcgt ttcttttggg atggcatgcc gatgcaaccg
     1441 cagattggcg tcagttactg ctatgtgcgc tcgccagtga atcatatcta cctgctgctg
     1501 ggagagctaa atacgatcgc cgaactttcc atcgtgacca acgccccgga aaatatgcag
     1561 cgtcgcgggg caatgtattt gcaacgcgaa ttgaaagata aagtcgcgat gatgaatcga
     1621 ctacagcagg cgctggaaca caaccatttt ttcctgatgg cccagccgat taccggtatg
     1681 cgtggtgatg tttaccatga aattcttctg cgcatgaaag gtgagaatga tgaactgatc
     1741 ggccccgata gcttcttgcc ggtcgcgcac gaatttggtt tatcgtcgag tatcgacatg
     1801 tgggtcattg agcatacgct gcaatttatg gctgaaaaca gagcgaagat gcccgctcac
     1861 cgttttgcta ttaatctgtc tccaacctcg gtatgtcagg ctcgttttcc tgttgaagtc
     1921 agtcagttgc tggataaata tcagattgaa gcgtggcaac ttatttttga agtcaccgaa
     1981 agtaatgctc tgaccaatgt taagcaggcg caaatcacct tgcagcatct tcaggaatta
     2041 ggctgccaga ttgcgattga tgatttcggc accggctacg ccagctatgc gcggcttaaa
     2101 aatgtgaatg ccgatctgct taaaattgac ggcagtttta tccgcaatat tgtgtcaaat
     2161 agtctggatt atcagatagt ggcatcgatt tgccacctgg cgcgaatgaa gaaaatgcgg
     2221 gtagtggcag agtacgttga aaacgaagag atccgcgagg cggtgctctc tttggggatc
     2281 gattatatgc agggttatct tattggtaag ccgcaaccgt taattgatac gctgaatgaa
     2341 atcgaaccca ttcgcgaaag tgcctgaata atgcgggccg acatttctcg tcggcccgca
     2401 aagcattaag cggcgatttc aggtgtactt tcttcttcaa ttttcaaccg ccagccagcc
     2461 acgccttccc agtattcttg ctccttttcc agatcaagca gtaccagcgc attctgacta
     2521 aaccagtcat gcgggaaacg cagtgtccag tgactgtcat cagtaatcag tgtcaatgtt
     2581 ggcggtgtgg tggttgcctg acgttgattg tttagtaata cgccaaggcg caatagctgt
     2641 atcagtggca ggaactgttt cttcttaaac aaggtaaagc gcggcagatc gtcgagctta
     2701 atcgctttac ggtgatagcg caccagtgtc gccatcatca gctgctgttc ctgattaaaa
     2761 cccggcaagt cactgttttg cagaatataa gcggagtggc gatgcaaacc gctgtggttg
     2821 atattcaacc cgacctcatg cagcatggcg gcccatcgca gtagcgcctc cagttgcgga
     2881 tgcgccagct tcggttgctg ttcccgccac tgttcgtaca tttgcatagt ggtatccagc
     2941 acccgtcggg cctgctcgct gtcgatgtga tactggttgg cgaggctgct ggcggtgcga
     3001 ctacgcacat cctgatgacg gaaacgtcct tccatttcat acagtacgcc ttcgcgtaaa
     3061 gccccgtcag aaaggcgcag ttcacggatg gctaaagcat caaacacacc gcataaaatc
     3121 gccagtccag gaacgaagac tgttttccgc tcttcggaaa gacccggtaa actcagcgat
     3181 gcgaaattac ggtgacgtaa aacttctttt accagttttt ccagacgttc cggggtaatt
     3241 atcccgtctt tctcgcccat ttccattaac acttcatggg cggcttttat ggtaccggaa
     3301 gcgcccattg ccacgttcca gccctgaata cggaattgcc aggttaaagt ttccagtttt
     3361 tgtgctgccg ccatacgagc gcgctgaaaa ttctctttat tgatgacccc gccagggaag
     3421 taaagctggg caaagctgac acaacccatc cggcggcttt caacgagaat cggttcgaaa
     3481 ttttcaccaa tcaccagttc cgtagatccg ccgccaatat caataaccag tttgcggcct
     3541 ttctccggtt gggtatgttc cacgcccata aaaatcagac gggcttcttc attaccggaa
     3601 ataatttcaa tcgggtaggg aatgaccttt tccgcgcgtt tcagaaagtc agtggcgttc
     3661 agcgcctgac gcagcgtatg ggtgccgact atacagacgc tggcaggaga aaacccttgt
     3721 agccgttcgg caaacagcga cagacagttc aaaccgcgcg tcattgcctc ttcactcaac
     3781 atattatctg gccccaggcc gtccgccaga tgcacccgct gtttcaggcg gccaataatc
     3841 tgcatggcac catctaccac acgggctatg accatgtgaa aactgtttga accaagatcg
     3901 accgcagcaa actcctgcgg acgaggggat ttatcgtgta ttggcatagg gttattcagg
     3961 ttgttcgagt gatttgatgt agtcataaat cgccaactgc gcccgtactt tgcggcgatt
     4021 gccgcgggga acgtagcgat tactgagttc tttatcgata taacgagctt tgaccgtatc
     4081 gctgaacaat atgtcgatga tgtccagcac ccgctgcttc aggcgcggat cgagcagcgg
     4141 tgtcgccact tcaatacgat aatcaatatt gcgcgtcatc cagtcggcgg aagaaaggta
     4201 gaccttttta tcgccgccat tttcaaaaat ataaacccgg tcatgttcta ggtaacggtc
     4261 aacaatactg atggcacgaa tgttgtcgct aatgccttcc agattgggga tcagcgaaca
     4321 cattccgcga accagcagat taaccggtac gccggagctg gaggccgcat acagacgatc
     4381 aaccaggcct ttatcgacaa ggttatttag cttcagggtg ataccactgg gcagcccttg
     4441 ctgcgcgttg gcgatttcgc ggtctaccat ttcatataac agacggcgag agttttgcgg
     4501 tgacaccatt aaataatcaa atgtcaccgg gcggtatggg ttttcaataa agttaaatac
     4561 ccgccgtact tcgttggtga tacgcgcatc ggcggtcagc aatgaatagt cagtataaag
     4621 acgcgcggtt ttttcgttaa agttcccggt cccgatgtgt gcgtaacgca ccacttcacc
     4681 gttttctttt cgtgaaatca ggaacagttt ggcgtgaatt ttcagccccg gcgcagagaa
     4741 gataacgtgc acgcctgctt cggtcaggcg cttcgcccag tgaatgttag cttcttcgtc
     4801 gaaacgcgcc tgtaactcaa ccaccacggt gactttctta ccgttatgtg cggcgtggat
     4861 catcgagtcg atgatgcgtg aatctttcgc cacgcggtaa atgttaattt taatcgccag
     4921 tacgctcggg tcgaacgaag cctgacgcag cagttccagc acatgctcaa aggtgtgata
     4981 aggataatag agcaacacat cgcgttcgcg aatggcatca aaaccattgc ggaactgggc
     5041 tttatcaaac caaatgtggc gtaaacgcgg cagtggtttg ttcaccagat tggctttgcc
     5101 gacattgggg aaattaataa agtctttaaa attatgataa cgaccgccgg ggacgatgga
     5161 gtcgtagcgg gaaatagtca gtttttcgcg taacacttca accagcgcat tgggcatatc
     5221 gcgctgataa acaaaacgca ccggctcagc agttaaacgc tgcttgagac tggaagacat
     5281 caactccatc aggctggctt ccatctcatg cactaaatcg tattcggcat cgcgggtcat
     5341 cttcattgaa taggcattca gcgcgtcata atcaaagaag cctttgaaaa tatcatcaag
     5401 gcagtaacgc agaatgttat ccagaagaat catcggcttg cgtcgacgcg gtgcttctgg
     5461 cggtaaattc acaaagcgcg gcactttatc tgatgggatc tccagcagcg cgtaacggat
     5521 ggtatcgcca cggataattt ccaccgccag ataggtgtaa tcatctttca ggaactgcac
     5581 taagtcagtg tcaggattga ttaaaatcgg cgtaatgtgc tgacgcagat actgcttaaa
     5641 ataatgacga agccagtttt gttgattgac ggagagctgg cgttcattaa tcaggaagat
     5701 ctggttacgc gccatctcca gcagcagctc gttgtagagg ccgtcgaatt cctgatcggc
     5761 tttcagcacc cgggactgaa ttttgcccag taaatggcgg gaatgagagt tggagccttg
     5821 ttcttcgcta ataatgatgc gtcgcttcag ttcagcgaag cggactttat agaactcatc
     5881 aaggttattg gaatagatcc ccaggaaacg catcctttca atcagcgggt tagatttgtc
     5941 cgccgcttcc tgaagcacgc gttcattgaa cgataaccaa ctgagctctt tttcgatgta
     6001 tagcttttcc tgacccatta ccacttttac tccgttttat tcacgggaca ctgcctggat
     6061 attatggcga gcaatattgg cagatgtcca actatgccag aaaaggggga aaaaaactgc
     6121 tggagcttgc gatttacagg tgaattgaaa aacgccagcg gcagagctgg cgctttaact
     6181 acggggcatt actcgtcggc agcgtagccc tgcggcggca gacgttgacc atccagccac
     6241 gcggcgtttt cgtgcatttt cagacgacca tcggcaaacc agctaatcac cagtggataa
     6301 atagcgtgtt cctgggtttg cacgcgggcg gtgatgtcat cttccgtatc accagcaaat
     6361 accgggactt tcgcctgtaa aataaccggg ccaccgtcca gttcatcggt gacgaaatgc
     6421 accgatgtac cgtgctcttc atcaccattt tccagcgcct gacgatgggt gtgtaatccg
     6481 ggatatttcg gcagcagaga agggtgaatg ttcagcaaac gcccggcata gtgggagaca
     6541 aacgccgggc tgagaatgcg cataaaacca gccagcacga ccacatcggg tgcgtacatg
     6601 tcgatttcat gaatcaactc ccggtcatag gcttcacgac tgtcaaacgc gctggcgatg
     6661 agcgtatgcg ttgcaatacc cgcctggcgg gcgcgttcaa ggccgaacgc gtcggcctta
     6721 ttgctgaaaa ctgcccgtac ggtgccttta attttgttag ttttacaggc gtcaataatt
     6781 gcctgtaaat tacttccgtt gccggaaata agcaccacaa tattcattat tcgataacca
     6841 cgcgttgttc ggaatcagag gctttgatga taccgatttt ccacgcgttt tcaccgttgg
     6901 cgtttagcag ggcgagggct ttgtccactt ccggagcagg cagggcgata atcatcccga
     6961 cgccgcagtt gaaggtgcga tacatttcat ggcgctcaac gttacctgcc gtttgcagcc
     7021 agttgaacac ttccggccac tgccaggaag attcatcaat cactgcctga gtattatctg
     7081 gcaatacgcg tggaatgttt tcccagaagc cgccgccggt caggtgcgca atggcatgca
     7141 catcgacctt ttcaatcaac tccagcactg acttcacgta aatgcgggtc ggtgccagca
     7201 gatgatcggc taatggctta ccatcaagtt cggtggtttg cggatcacaa ccgctgactt
     7261 caagaatttt gcgcaccagc gaatagccgt tcgagtgcgg accgctggaa ccgagtgcaa
     7321 tcagcacatc gccgtcgctg actttagagc cgtcgatgat ttctgatttt tctaccacgc
     7381 caacgcagaa acccgcgaca tcgtaatcct caccgtgata catccccggc atttctgccg
     7441 tttcgccacc caccagtgaa cagcccgatt gcagacatcc ttccgcgata ccgctgatca
     7501 ccgctgaagc ggtatcaaca tccagttttc cggttgcgta atagtcgagg aaaaacagcg
     7561 gctctgcacc ttgcaccacc aggtcattaa cgcacatggc gaccagatca ataccaatgg
     7621 tgtcgtgacg ttttaagtcc attgccagac gcagcttggt acctacgccg tcagtgccgg
     7681 aaaccagcac gggttcacga tatttttgcg gcaatgcaca cagcgcaccg aagccgccca
     7741 gaccgcccat cacttccgga cgacgcgttt tcttcactac gcctttgatt cttccaacca
     7801 gagcattacc cgcgtcaata tcaacaccgg catctttgta gctaagagag gttttatcgg
     7861 tcactgcttg ggtccccacg cgttacttgc ggtagaaaaa taaaattcgg cgcaattcta
     7921 acagggaaag caaacgtttg cgagactgct ttacacaacc tttttgcacg tcttttgccc
     7981 aggcacgcgg cgaaagaaga cttgtgccag ggtaaaggtt agttttcgga tggaataatc
     8041 ttctttcata accatttgaa tataaaataa ctttatctca aatcgttatc attttgacta
     8101 aagtcaacga aaagaatatt gccgccttga agaaaggagg tataatccgt cgattttttt
     8161 tgtggctgcc cctcaaagga gaaagagtat gaagatcgtg gaagtcaaac acccactcgt
     8221 caaacacaag ctgggactga tgcgtgagca agatatcagc accaagcgct ttcgcgaact
     8281 cgcttccgaa gtgggtagcc tgctgactta cgaagcgacc gccgacctcg aaacggaaaa
     8341 agtaactatc gaaggctgga acggcccggt agaaatcgac cagatcaaag gtaagaaaat
     8401 taccgttgtg ccaattctgc gtgcgggtct tggtatgatg gacggtgtgc tggaaaacgt
     8461 tccgagcgcg cgcatcagcg ttgtcggtat gtaccgtaat gaagaaacgc tggagccggt
     8521 accgtacttc cagaaactgg tttctaacat cgatgagcgt atggcgctga tcgttgaccc
     8581 aatgctggca accggtggtt ccgttatcgc gaccatcgac ctgctgaaaa aagcgggctg
     8641 cagcagcatc aaagttctgg tgctggtcgc tgcgccagaa ggtatcgctg cgctggaaaa
     8701 agcgcacccg gacgtcgaac tgtataccgc atcgattgat cagggactga acgagcacgg
     8761 atacattatt ccgggcctcg gcgatgccgg tgacaaaatc tttggtacga aataaagaat
     8821 aaaaataatt aaagccgact ttaagagtcg gctttttttt gagtaaagcg cctataacac
     8881 ataatacaga ggataatact atgacgcgcc gtgctatcgg ggtgagtgaa agaccgccac
     8941 ttttacagac aatcccgctt agtttgcaac atttgttcgc catgtttggt gcaaccgtcc
     9001 tggtgcccgt cttatttcat attaacccgg cgactgtact gttatttaac ggtattggaa
     9061 cgctgctgta tctcttcatc tgtaaaggga aaattccggc ttatcttggt tccagctttg
     9121 cctttatttc accggtattg ttactgttgc cgttagggta tgaagtcgcg ctgggcggct
     9181 ttattatgtg cggcgtgctg ttctgcctgg tttcttttat cgtgaagaaa gcggggaccg
     9241 gctggctgga cgtgctgttt ccacctgcgg caatgggcgc aatcgttgcc gtcatcggtc
     9301 tggagctggc gggcgtagct gccggtatgg cgggtttact cccggctgaa gggcaaacgc
     9361 cagactccaa aaccatcatc atctctatta ccaccctggc ggtcacggtt ttaggttccg
     9421 tgctgtttcg tggtttcctg gcaattatcc cgattttaat tggcgtgctg gtggggtacg
     9481 cgctctcttt cgcaatggga attgtcgata ccacgccgat tattaatgct cactggtttg
     9541 cgctgccaac cctctatacg ccgcgcttcg agtggtttgc cattctgact attctgccag
     9601 cggcgttagt ggttattgcc gaacacgtag ggcacctggt agtaacggct aatatcgtca
     9661 aaaaagatct gctgcgcgat ccaggtctgc accgttcgat gtttgctaat ggcttgtcga
     9721 ccgtgatttc cggcttcttt ggctctacgc caaataccac ttacggagaa aacattggcg
     9781 tgatggcgat cacccgtgtt tacagtacct gggttatcgg cggggcggcg attttcgcta
     9841 tcctgctttc ctgcgtcggt aaactggctg ccgctatcca gatgatccca ttgccggtga
     9901 tgggcggcgt ttcgctgctg ctttatggtg tcatcggtgc ttccggtatt cgtgttttga
     9961 tcgaatcgaa agtggattac aacaaagcac agaacctgat cctgacttcc gtgattttga
    10021 tcatcggcgt cagtggcgcg aaggtaaaca tcggcgcggc ggagttaaaa ggtatggcgc
    10081 tggcgaccat cgtcggtatc ggcttaagtt tgattttcaa actgatcagt gtgctgcgcc
    10141 ctgaagaagt ggtgctggac gcagaagacg cggatataac agacaaataa tgtaccgacc
    10201 gggcagtgtt gctgcccggt tcaaacatca tgggattctg tggtaaactt ctcgcgattt
    10261 tgtgaaatcc tggttgaggt atctctgaac acaccggcac agctctcttt gccactttat
    10321 cttcctgacg acgaaacctt tgcaagtttc tggccggggg ataactcctc tttactggct
    10381 gcgctgcaaa acgtgctgcg tcaggaacat agcggttaca tctatctctg ggcacgcgaa
    10441 ggcgcggggc gcagccatct gctgcacgcg gcttgcgcgg aattgtcgca gcgtggcgat
    10501 gcggtgggct atgtcccgct ggataaacgc acctggtttg ttccggaagt gctcgacggt
    10561 atggagcatt tgtcgctggt ctgtatcgac aacattgagt gtattgcagg cgatgagttg
    10621 tgggagatgg cgattttcga tctctacaat cgaattctgg aatcgggcaa aacacgattg
    10681 ttgatcaccg gcgatcgtcc accgcggcag ttgaatctgg gattaccgga tctcgcgtcg
    10741 cgactcgact gggggcagat ctacaaattg cagccacttt ctgatgaaga taagttgcag
    10801 gcgctacagt tacgcgcgcg tttgcgtggt tttgaactgc cggaagatgt ggggcgtttc
    10861 ttgctgaagc ggctcgacag agaaatgcgc acgctattta tgacgttgga tcagttggat
    10921 cgtgcgtcga ttaccgcgca acgtaagctg accattccgt ttgtgaaaga aattctgaag
    10981 ttgtagataa ggtgtttatt gtcggatgcg gcgcgaacgc cttatccgaa ctacgcgaag
    11041 ttaatccata gtattcgtag gccggataag acgtggcaag cgtcgcatcc ggcaatgtgc
    11101 ttcaactagc gatggagcac atcttaaccc acgatttcca gtacctgctc cggtggacga
    11161 ccaatccgcg ctttgccatt cgcgaccaca atcgggcgct ccattagctt cggattatcc
    11221 accattgcct gaatcagcgc ctcttcgcta agcgaactgt cagccaggtt cagttcttta
    11281 taaagatcct ctttctggcg catcaattca cgggcgctat tcatccccag cattttcagc
    11341 aaatcgcgca gcgttgccgc atcagcgggt gtctcaaggt agagcaccac ttctgactcc
    11401 acgccgtttt ctttcagcag gttcagcgtt tcccggctct ttgaacagcg tgggttatgg
    11461 tagattttta cctgtttggt cattgttctt cctttaatgc gaattacatc ttggtataag
    11521 gcttaaagcg ttcctgcagc tggcgcaact ggtcgatgcg cgcatcgtaa cgcgcttgtt
    11581 gcaggctgcc taatttcacc tgcgaactgg cgctactcaa cagcgaaatg gcctgatcga
    11641 gtcgtccggc gagcgcataa ccttctgctc gcgcagccag ctcctgatcg cggttattta
    11701 gcgcggcttc cgcctgtgcc agcaaatccc agccgttgct gtcatcttta ttattaaagg
    11761 tgtagcgatt gagaatattg gccgcttctt gtggttgacc gccttgcaga taagcgttcg
    11821 ccaggttgag ctgcaacacc ggattggtgc gtaaatcgcg ggcatttttc agacgattga
    11881 tcgcctcatt ggctttgttt tgcccaagat cgatatcagt agccagatcg agataccatg
    11941 cgttgccagg ttctgccgcc agtaacggtt gcagcgtttt acgcgcctcg tcgtatttat
    12001 tggcttccat cgcctgtaaa gcacgaccat attgtgctgc tcgttgctga cgaacgtttc
    12061 ctttcgccca ttcatccagc aaatcactgg tgagctggtt acgtccggaa ttatacatcc
    12121 ccagtgtgcg cgctttcgcc agatagaaat cttccgacga ctgcaccacc atcgggcgca
    12181 tctgattagc acggttgcgg gcatctgcca gacgactttc cggcaacggg tgagtcaata
    12241 aaatttccgg cggacgcgag gagtaacgcg cctgatcgag taatttttcg aggaaggttg
    12301 gcatcgcctg cggatcgaat cccgagcgtt gcagcacctg aataccaatg cggtccgctt
    12361 cctgttcatt ttgctgggtg aaactgatca tcccctgacg cgttcccgcc agtgtaccgg
    12421 tcagcgccgc catccccgcc tgcggactgg ccatcgccag taaaatagaa cctaacgcgc
    12481 cgacccaggt cagcggcgcg ctgcgctgct gatcttccat cgctcgcgcc aggtgacgtt
    12541 gggtgacgtg ggagatttcg tgcgccataa ctgaagccag ttgactttcg ttatcggaat
    12601 aacggaacag ggcagagtgc agcaccacgt tgccgccaaa gaaagcaaag gcgttaattt
    12661 cgtcgttgtt gatcagaaag aaatgaaacg gtgtcttaac cgaattggca tgcgaaacca
    12721 gacgcatccc cagcgaatta atatattgcg ttaacagcgg gtcattaatt aacggcgcgc
    12781 tgccgcgtag ctggcggaca taatagtcgc ccatctgcat ttcctgacca atggaaagcg
    12841 tgcttcctgc ggaggttccc atatccggca aggtgtctgc gctgtcggca aacgccgggg
    12901 ctacctgacc aatggtcata gcagcaatga gggttgcaac caggtttttt ttcaactgcc
    12961 tgaacataac ctctatcctg tatttctgaa gatagtcatt tgaccgatac tgtgaaatat
    13021 cgttcccacc gcgccgcctt tgagtgtaac ggcgaagatt agcccactca aatgactttt
    13081 cagtttcctg aatccgtcat aaaaagcgaa gtcttttttg tgacatttcg atacaattgc
    13141 ggatcgcaat cccgctcttg aggttcaggg aaggtcttta tgctcgaaat gttgatgcaa
    13201 tggtatcgcc gccgttttag cgacccggaa gcgattgcct tgctggttat tttagttgcc
    13261 ggatttggca ttatcttttt ctttagtggc ctgcttgctc cgttgctggt ggctattgtg
    13321 ctggcctatt tgctggaatg gccaactgtg cgcctgcaat ctattggctg ctcccgccgc
    13381 tgggcgacgt cgattgtatt ggtggttttt gtcggtatat tgctactgat ggcgttcgtg
    13441 gtactgccta tcgcctggca acagggcatc tacttaattc gcgatatgcc ggggatgctc
    13501 aataagcttt ctgactttgc cgccacgttg ccgcgccgct atccggcgtt aatggacgcg
    13561 ggcattattg atgcaatggc cgaaaatatg cgcagtcgga tgctgaccat gggcgattcg
    13621 gtggtgaaaa tttccctcgc ctcgctggtc ggtttgctga ccattgccgt ctatctggtg
    13681 ctggtgccat tgatggtctt cttcctgctg aaagacaaag agcagatgct gaacgccgtt
    13741 cgtcgggtgc tgccgcgcaa ccgtggactg gcaggacagg tgtggaagga gatgaatcaa
    13801 caaatcacca actatatccg cggcaaagtg ctggagatga tcgtggtggg gatcgccacc
    13861 tggctggggt tcttgctctt tgggctgaac tattcgctgc tgctggcggt gctggtcggc
    13921 ttctcggttc ttattccgta cattggcgca tttgtggtga ccattccggt ggttggcgtg
    13981 gcgctattcc agtttggcgc aggcacggaa ttctggagct gcttcgcggt gtatctgatt
    14041 attcaggcgc tggacggcaa cctgctagta ccggtgttgt tctccgaagc ggttaacctg
    14101 catccgctgg tgattatttt atcggtggtg atcttcggtg gtttgtgggg attctggggc
    14161 gtattcttcg ccattccgtt ggcgacgctg atcaaagccg tgatccacgc ctggcccgat
    14221 gggcaaatag cgcaagaata atcttcagcc cgacgaactg tttcgccggg ctgatttttc
    14281 aggattcctg acgtaaataa atagcccgat aacacatact caccagcact gcaccgccga
    14341 taatattacc cagcatcacc gggagcagat tggcggtaat aaaatggctt accgtcagtg
    14401 tcggaaaatt gtctgcgcta ctgtgcgcca gttgccagaa ggaggtaggg gcgaaatggc
    14461 gaatggcaat ggcgaacgga atcacaaaca aattggcgat acagtgctca aagccactgg
    14521 cgacaaacag ggtgatgggc aaaatcatgg cgacgatttt gtcgcataac gaacgcccgc
    14581 aataactcat ccacagcgcc aggcaaacca tcagattgca cataatgccg aggctgacag
    14641 attcagtaaa tgtatgatgc attttgccct cggcgcagtg taaaaccgcg actccccaca
    14701 tcgcgttttc actcatcacc agcccggaaa accagattaa caaactgaaa caggcaatac
    14761 ctgccagatt accgcaggcc accagaagtg cgttaatcag ccaagttcgc caactaataa
    14821 cgccccgact ttttgccatt accgtcatta ccgacgaggt gaacaggttg gtgccgcaaa
    14881 ccgccagcaa aataaagcca agtgtaaagc aaaggccgcc caccagatgg gttaatgcct
    14941 gtgaagacgg ggcatcggcg ataactgaaa ggtaaaaagt aaatccgatc gccataaaca
    15001 cgccagcgga catcgccatt aaaaagaccg acactttact gcgggcaatt ttatgggcgg
    15061 caatccgttc agcgattgcc gcttttctgg cgctcaactg caagtcgaaa gagagtttgt
    15121 ttctcatgca tactcctgca aaagcagatt acaacacctc gcgaaccgag attcccagac
    15181 gctgcattcg tgacagcagc gtggtgcgct tcatccctaa tcgtgtcgcc gcgccacggg
    15241 gcccggcaac aatgccattg gtttctcgca atacctgaac aatgcgctga cgttcttctt
    15301 cgtcattctc tggcgtcgtc gggtgcagca actgcgccat tgaactgcga agcgctgaat
    15361 cttcatttag cgtcggcagt aaacggcttt gtcggacatt aagatgtaaa ttcagactgt
    15421 taccgcgagt cagcagtacc gctcgctcaa tcacgttttc cagctcgcgc acgttgcccg
    15481 gccagtccca cgacatcaac tggcgtagcg cctcggtcgg gatggcgtca attgaacgat
    15541 tcatatggcg cgccattttt tgcgtgaaat gctttgctaa aagagggata tcttccggac
    15601 ggtcgcgcag cggcggcaat tccagtggga agacattcag gcgataaaag agatcgctgc
    15661 gaaactggcg atcttcaacc atttgccaca aatcacggtt ggtggcggca atgactctga
    15721 catttaccgg gatcgttcta ctcccgccga gacgctcaat ctcccgttcc tgcaatacgc
    15781 gcagcagttt aggctgaagt tctaacggca gatcgccaat ttcatcgaga aacaacgtgc
    15841 cgccatcggc aatttcaaaa cgaccacgat gggtattaat cgcaccagta aacgcccctt
    15901 tgtcatgacc gaataactca ctttccagaa gactggcggg gatggcagcg cagttgattt
    15961 tgaccagcgg cttgtcgcgt cgcgggctaa gttgatggat cgctctggcg atcacctctt
    16021 tgccggttcc ggtttcaccg caaatcaaca ccgtactgtc gctcttcgcc acaatatcta
    16081 cctgctggag taggtcttcc attgcctggc tttgatagat aatgtcaccg acgcccagat
    16141 tcgaaaggag ctgctcgcta agctggtggt tttcttgctg caaactttcc tgcaaatcgg
    16201 tcatgctacg ccaggcatcg gcattgccaa cggcaatagc gatgcgatcg gctatgtgtt
    16261 gtagtagctg gcagttttcc tcactaaaga gagtggaaga ggtatgcgcc agtaacaatg
    16321 cacccggtgt atggttgcca aaggtaagcg gtatcaggag cgcagattcg cagccgttag
    16381 atgcaagtaa gagtaataac gggtcgcgtt gccagagaaa cagatcgtct gcacggtgcg
    16441 tcaaggtcgg ttgtcgggtt tgtagcgttt gtgtcagcaa tacactgtcg ccaggcatat
    16501 tgcgtggcag acacgcacaa tgtgaggcag aaagatcgct gcaccacagg ctgaacttct
    16561 cgttctttcg atgatcgccc agtaccatac tgactgaagc cagaccgaaa aaatgatgga
    16621 tctcacgagc gacgtcagcg atcagatcgt cgagatcaag atgtgacagc actgcattag
    16681 tgatatccac cagtacccga tgatgatcgc gatctttctc cagcaaacgc agcgccacct
    16741 gttgttcttc atcattgagc catgattgca gaaccggggt gataatgcgt gccagccagt
    16801 taaacagtgt gcaatcctgg ggagtaaaac ggctgccgtg ggtacgaata aagcgtagct
    16861 ccgccattcc actactgccg agaaggacgg cgtgacgcga ataatcgacc atctcgccct
    16921 gatactctac tgttgccgtc acatcgctgc catcatcatg gtagcggtaa tagaagttgc
    16981 ttttgagaac atgcagttca ataagttgta ccgggctgaa cggcggcggc agcgggatga
    17041 aggcgcgtaa taaagacgcc ttgccgtgtt tctgtgctaa ccgctccgcg agcattccgt
    17101 ttaccgcttc aattgttatt ccttgtggcg gggcaaacat cgcctcgtct gacatagcca
    17161 tcaacagtcc ttattaatga gaaatccttc tcacgcaatt tttctggcca tcagatagat
    17221 ggccggttcc tgcacaattt catccaacag atgaatgaat tgaatgctac gcgcctgcca
    17281 cacagacgtc gcgtcatctg ccagtagcgc gaatgcccgg gtcaacaggg cggtgtggct
    17341 ggcgtcaata acaatctcac caaaggtcat cacccccagc atcttacgtc gggcttgctc
    17401 ctcttcgacc tgtgcaagcc aatcttcgta ttccgtcagt gggcagcgga aggcgacatt
    17461 cagacaatca atcacgccaa cgtgatgacc gatagccagc gagtaataca ttacctgaga
    17521 gctgtgttcc ggcatctcgt cgctactggc gacaaacttt tttcgcagcg tccagaaaac
    17581 aatttccccg cactcctcag ccatgtaacc cccggagaac gcgaaagagt tgctgaacga
    17641 tactattgag acgcggatcg tcggcttcgc gcaaccaggt gttaacccga tttcctgtag
    17701 gatcgtcgct taaatggcgc aggagccgat cgcaaatttc tcgcccctga cgatagcccg
    17761 ccagccgccg cgcttctcgc tcaatggcga tacgctgtga tggcgggatc tgtggccaca
    17821 acggttgcat agtcaccccg gtgggatcgc gataatccac agcgtgaatc ttctgttgca
    17881 gcaaaccgag cgccacggcg aaaccgtgaa tggtggcggc cggtgttggc gggcagccgg
    17941 ggatccaaac atcaatgggg acaatggtgt cgctgccgcc ccagacgctg tagagatcgt
    18001 ggaaaatacc gccgccgaca ccgcacgcgc cgtaggaaac acagatttta tgatcggggg
    18061 cagactcata cgcccgaagt gcaggcatac gcatcgcccg ggtgactgcg ccagtaaata
    18121 acaaaatatc ggcgtgacgc ggtgatgaaa caaccttaat gccaaaacgt tctgcgtcga
    18181 atactggtgt gatggcagca aaaatttcga tttcacaggc gttgcagccg ccgcaatcga
    18241 cgcgataaac gtaagccgag cgacggatat cctgtagcaa atgctgcttc atcttttgcg
    18301 tttgctcgtc cagcgtgatg ggctggctga catattgcgt aagcactgga ctcatagctg
    18361 ctccttagcc accagcggta catctgtatc gtcgttgatc agcgtcgcgc gttgtttgca
    18421 ctccgggcag acgctcgctt gcgcccgcaa catttcgcgg ttttgtggcg cattttgttg
    18481 ctgtgccaac aattcagcag ccagtgcgac ggttttttgc ggggcaaacg ggcgttcgca
    18541 acggctgcaa cgttgtagat ggaacgtcgc gcgggtatag agatcggctt tattggtgac
    18601 ggtcagttca aagttattgg taagctggat ggctctggtc ggacacactt cttcacaacg
    18661 tccgcagtaa atacaacgcc ccagatagag ctgccaggtg cgcgaatttt gctggtcgtc
    18721 ggtctggata gtcagcgcat ttgccggaca agcacaggcg caggcaccgc aggcaataca
    18781 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
    18841 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
    18901 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
    18961 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
    19021 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
    19081 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
    19141 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
    19201 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
    19261 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
    19321 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
    19381 gtagtcggcg taggggtgat caaagcgcaa atcacgggcg atttgtcggt cgagaatgcc
    19441 aatgccctga gtgcgttgtt ccatattcgg cgtagcaagc agcatctcta ccagctccga
    19501 cacgtcggcg cgcatctcgc gcaccagttt cagcgtttgc agacgttgct ctttgagaat
    19561 atcgcggcga acaccaccaa tcagattcag accgtaggtt ttacgcgacc cgatcagcaa
    19621 ttccgccatc gtcatcgact tttcccgcac gcggaaaaat tgcataaagc cggtatcaaa
    19681 accaacgaaa tggcaggaga ggccaaggtt aagcaaatga ctgtgtagcc gttcgacttc
    19741 cagcagaatc gagcgaatag tatgtgctcg ttgcggcacc tcaatcccca gtgcattttc
    19801 aaccgaattg gtataggcca cactgtgggc aaaaccgcaa atcccacaca cgcggtccga
    19861 taagaaggtc acttcgttgt agcccatccg cgtttctgcc agtttctcca tgccgcgatg
    19921 gacataaaac aggcggtaat cagcatcgac aatttgctcg ccatccacaa acaagcggaa
    19981 gtgacccggt tcatcggagg tgatatgcag cgggccgaca gggatcaccc gcgcatcgct
    20041 gttgccctca ttgataaacg catacgtttc ggaatctgtc gtcggttcag ggcgcagtcg
    20101 ataatccatc gcatctttgc gcagcggatg catatcttcc ggccagtcat cgggcaacac
    20161 caggcgacgc tgatccggca ggccaaccgg aatcagcccg tacatatcgc gaatttctcg
    20221 ctcgccccag accgcggcag ggacgcgcgg tgtgactgac ggaaactccc gactgtcggc
    20281 atcgaccagt gccttcacca cgatccagca tttttcggcc ccttccattg aaagggcata
    20341 ataaaccgcg taatgaccgt taagtgtccg ctcgtcgttg ccaaacagga ctggaagcca
    20401 gccatcatgt tgataataaa gataatgtac aacgtcaggc agcagattga ttttcaccgt
    20461 aatggtgacc tgttcaggcg tttgtcgctc ttcatccagc tccgcgccgg ggaactgcgt
    20521 tttcagggcg gcgagaatcg cttcgccacg atttgacgat gaattaacgt tcactgtgtt
    20581 tctccgaaac agatgcggtg cctgaaggca aaaaatcatg ccaggtgcta cgttgtgcag
    20641 gcaggtcgtg cgtccctgag aggactatag tggaagcgcc agccaggatc ctgatgacag
    20701 gttgtggaat atgcgttccc atcgccagca tcatgaccag cagaatcacc attggcgagg
    20761 tggtcaacca gccgagatca ccccggttaa cggcctgcgg cggtttcgcc attaacaccc
    20821 gcgcagccat ccgtaccagg cccgccagca ccagcgttaa cagcaataac agcaggacga
    20881 taatcagcag gtgattacgt gccagtcctg cggtaacggt cataaattcg ctaagaaaaa
    20941 tgttgaaggg cggcatccct gccagcgcca gcgcaccgcc gccaaacagc acggcggtaa
    21001 atggcatgat tttgagcatc ccacagacga cgttgagatc gcgcgtgccg tacttgagca
    21061 gtacattgcc ggaaccgcag aacagcagcg tttttgccag actgtggttt aaggtgtgca
    21121 gcagcgcggc aaaaattccc agcggcccgc caatgcctag cgccaccgcg accagcccca
    21181 tgttctccac gctggagtac gccagcagac gcttaatgtc ccgctgtacc agaatgaaaa
    21241 atgccgccac ggcaaccgac aacatgccga agatgagcaa caaccggttg gggaaatcgc
    21301 tgccgatggc ttggcaaata atgatgtaat agcgaatcag caccaacagc gcgcagttca
    21361 gcaatacggc ggagagcagg gcgctgaccg gactcggtgc ttcactgtga gcatccggca
    21421 gccaggcgtg catgggaaat agcccggttt tggtaccaaa gccaattagc acaaacacaa
    21481 aggccaacag cattaatgtt ggttcaagca aggacgattg cttaagaacc tcgctccaga
    21541 atatcgccat ttctgcctgc ggcataacgc tggcggcgtt ggcgtatacc agcacggtac
    21601 cgaacagacc aaaagcgaca ccaacagtac aaataatgat gtacttccat gctgattcca
    21661 gcgatgaacg ctgaccgtaa atgcctacca gaaacgccga gcttaaggtg gtggcttcga
    21721 ttgccgccca catcacaatc aggttattgc tggtaacaac cagcagcatg gtgaacaaaa
    21781 acagatggaa gaaaccgtag taatcgcaca gcgtaacggg tgaaagctcg ccgtgtgcca
    21841 cttcatgacg catgtagcca atcgagtaaa caccggtgag aaagccaatc acaccaagaa
    21901 tggcgaggaa caaaccgccc agaccatcaa tatgaagcca cagtcccgca gcgaatattt
    21961 ctcctgcatc agcggcagtt tggaccaccc agagtgccag aatcagcagc agtgtgatcc
    22021 ctaagctatg taatacagtc accgtacaag tcgcagagag cccccgtttc cggcaggcaa
    22081 aacagagcag cgaaaaaagc agcggcgtga gcaggagtaa agcgaacatc acagaataac
    22141 tcatctcatt atcccttcag cgcggtcaag ttgttcacgt ccagcgtgcc gtgggtacgc
    22201 catatttttc ttgccagtaa caccatcaca atgacggcga agatggcgtc ggtagcgata
    22261 gctatttcca ccagttccgg tgctcgccag gcaagaagcg ccagcaccag atgggagccg
    22321 ttttccatca ggcagtaacc aaaaatttgc tgcaggatat tgcgctggct gacaatgcac
    22381 agcaggccaa gcagaaaatg acctaacgct accgccagcg ccggtttcag cccggtagcc
    22441 atcggtagct tcacgggctg aacgacaaat gcgcaaagca ggacaattaa cgccgcgagc
    22501 agtgccatca ttgccggacc gaataacgct ttttccggga tgttctgggg aatatttcgt
    22561 gcagcgtaag tcatgattaa cggtaccagc agcactttgg tgataaaggc gctggcggac
    22621 cagatcagca gttgctctgc ggcgaacagg cacgagagag tggcgaaaat agacaccagc
    22681 accagcgact ggcaggcgta aaatccgcat gacaggcgat agcttttgac gctaatcaca
    22741 aacagcgatg tcagcatcat cagtcccgcc agattattta cgatcataga accggtcatt
    22801 catcatctcc ttactgcaac caggtcgcgg cgattacgct ggaaatcagc gacatcacaa
    22861 tcagtacaat caacaccagg cgcattgacc ctggcagcgg tgcggcatcg gcgacggcct
    22921 cgctcggttt gccaggcaca acgcgaccaa accagcgaat aaaccaggcg aaactggcga
    22981 ccgattcaat catcagcaga atcatggcgg gcagcaggat ccagtactcc actgacaacg
    23041 caaaaccggc agcaaacagc gggaatttac tgaagaagcc gttgaacggc ggcacaccgg
    23101 taatcgccag cgctgccacg cagaaaccca cgcctggcaa cggcagggtg tgcaatacgc
    23161 cacgcagacg cggcaacaag cgcgtgccgc agctgtaact cagcgcacct gctacaagga
    23221 aaaacaggct tttagcgaac gcgtggttga cgatgtaggc gatgctgccc tccagtgcca
    23281 gccgcccact ttcaccttcg atgcggcgtg gagataggca ctgatcggtg ttggcgcttc
    23341 cattgcatcc ggtagccacg cttgcatcgg tagctgggcc gatttccccc acgcggcaaa
    23401 caggatgccg ccataaacca gataacgtgc gtcgccgtgt aactcgctca tcgcgctaag
    23461 cgcaaacgtt ccggtctgca aaaacagcgt ggcggcggca agatacaacc ccaacgagcc
    23521 gatatgagtg ataagtaacg cttttagtgc tgaacgctgt gctttatcgc tctggtaata
    23581 actgatcaac gcccaggagc agccgcccgt aatttcaaaa aacaacaact gaccgagcag
    23641 cgtcgaggag agtaccagtc ccgccatcgc gccgataaac aacagcaaaa atgcgtaata
    23701 acgattcgtg ccgttatgcg ggtgttcgcg atttttatcc gtcagataac ccgtggagta
    23761 gatcgtgacc agcaaaccga gaaacaccac cacaaacaga atcagcgtac tcacgcgatc
    23821 aatgaccagg ccaaacaatg ccacatcccc cacgttgacc aatgtcaacg tgacggcaac
    23881 tttatcggcc tgataaaacg cggagatcag tgacaacatg cacagcgtgg tcagcgcggc
    23941 gaacaaaacc ccccattcgg cggcccgacg ttgtggcgaa aacgaaacga ccagtgcgcc
    24001 gataaaaggc agcaataacg tcgtcagagc aagattttcc atattccgtc agtgctcctt
    24061 acagaccggt taaccagaag acccatgcaa gcgcagcaag gctgaagcca agccaggtca
    24121 catggtgaat gagtaaaaaa cgcccgcgtg ccagcgtgtt ttctgcgatt gaggccagta
    24181 caaaaatcag caaaaccttg agcagcgtaa cgacaagtga agtcagcagg caggcgagag
    24241 aaagttcttg cgcgcgccca aagggcagaa acagggccac aaacagtgat gccatcacga
    24301 cctgtttcag ccccagcccc catttcgcca gcgccagccc ggcaccggaa tattcggtca
    24361 gcgggccttc ctgtaattcc tgttctgctt cagcaacatc aaagggaatt tttcccatct
    24421 caatgaagca ggcaaaacca caggccagta acgccagtac ggtggttagc ggcgagttcc
    24481 agcccatcgc cagcgtattg ctgatcatct cgatatgcgt ggaacctgct atcagcgcca
    24541 ataccagcag tgagagaata agcattggtt cgaccagaat gccgagcgtc aactcgcgac
    24601 tggcaccgac tcccgcaaac ggacttccgg tatccagccc ggaaagagca aagaaaaaac
    24661 gaaacagggc aagaagatag ataagggtga tcagatcgcc gccgcccgca aaaggggaaa
    24721 cggtaataaa cagtggtaag gccatcgcca gcaccagcat gctgctgatt aatacccacg
    24781 gcatcaggcg gaacatcaga cctgaagatg tcggcgcaac ttcctggcgt ttaaacagtt
    24841 tgtggatatc gcgataatcc tgccagatcc ccggcccgcg gcgggagtgc atacgcgcgc
    24901 gtatctgccg ggaaataccc gtaaaaagtg gggttagcat cagcagaatc acggcctgtg
    24961 ctaacgcaaa aataaccaga tatccgtcgc aaagagtttg tctcatggtg atttccttag
    25021 acggcaatag cgattagcag cacaaccagt gcggcgacca catacaggca atagagacga
    25081 aagtcgccgc tttgcagatg ctgtatttct ttggccagcc gttgggtggc gctcacgatg
    25141 gggcggatca cccgctcatc ccagaagggt tctgtgctct gagccctggc ggtgacgtgc
    25201 gcaaaacctt tatttagcct cagcgtaggg tcgagctgtt gtcgtagacg aaataacgca
    25261 gagaagacca cacgcagcgg ctgcatcacg ccattgcctg acggggccat cgcattttcc
    25321 cagccgtagc cgcatgccca ggcatctcct gtgcgacgaa acgctgcgcg gcgcgaacga
    25381 caccacagcc agtaaaggcc aggcagcaaa ggcatcgcca gtagtaacag caacagtaat
    25441 gagggggtga cctgtgtatg aaacgtgccg ggtacaagtg ctattccgct ggcgacagtg
    25501 acgggagggg tattggtaaa cgcatgggca atatgcatta tcttcggtgc cagccaactg
    25561 gcactaagcg caattaatac gcagagtgcc gcgagcagta gcatggcgac gatcatcgta
    25621 tttggcactt cctgtgcctc ttcagcgtgt gtactgcgcg gcgcaccaca gaaagtaata
    25681 ccgtacattt ttacgaagca cattactgcc agcccaccag tgactgccag cattaccata
    25741 gcaataggac ccgcaagttg tagcgctacg gcttccacac gacttagtga gaacagcgac
    25801 tgccaggtgt accattcgct gataaaacca ttcagcggag gaatggctga tatcgcgagg
    25861 caaccaatca ggcatgctgc ggctgtccac ggcatccgtt tcgccagtgc ccccattttt
    25921 tccatgtcgt gggtatgcaa acgcgaaata atcgcgcccg cgccgagaaa tagcagccct
    25981 ttgaacagcg catggttgag cagatgaaac agtgcgccga gcagtccaac cacggtgagc
    26041 agcgggtcgt gcagtgacag accgaccatc gccacaccga ctgccagcaa aataatgccg
    26101 acgttttcga cggtactcca ggccagcagc cgtttgatat cctgttccgc cagcgcatac
    26161 agcacgccca ggagcgcgga gattgcgccg atcgccatca ccagaatgcc ccaccacagc
    26221 ggcaaacccg tttgcgccag caggtccatc gctactttca ggatgccgaa aataccgatt
    26281 ttgaccatta cgccagacat caacgccgaa gcgtgcgatg gtgcggcagg gtgagcgcgc
    26341 ggcaaccagc tgtgcaacgg catcatcccg gctttcgcgc caaaaccgaa aaaggccagc
    26401 agaaacaccg ccgacgccag ccccggagaa agtgaaagcg tgcgaaactg gcaaaatcga
    26461 ggctgccgct ttcgcgcccc atcagcaaga aggcgatcat aatcagcacc gatccggcgt
    26521 gggcgataaa aaagtagagc atgccagcgt tgatcgacgt tttatcctgc ctggcaatga
    26581 ccagaaacca ggaagacagc gacatcattt caaacagcac gatgaaccaa aaagcgttgt
    26641 ccatcaccag cagggcaacc atcgatgcga tgaaaagatt cataaagaag cccatcgccg
    26701 ccgcgccttt gccctcgtat tcgcgcatat aagtcaatga atagagcgaa caaaccacca
    26761 ccagcaagga gataaccagc accataaatg ccgagaggct atccattcgc agggttaaat
    26821 cggcaaagtc aaaaggggtg gcaaaatggg cgactaaagt tacaccagca tgtaattgcg
    26881 taattccgct gatgatgcca atcacgccgc ccaccagcga tgtgatgccg gaaagcttaa
    26941 tagccagtct gtccagaccg agtaaaaaca gcgaagccag actggcaaac agatagagga
    27001 tcagcgacca ggttaataat tgcagggcat ccattagcgt tgctcctgag tgagggaaag
    27061 gggatcctcc ccgccattgg caaaccagct tgcggcaagg cgctgttttt ctttcatctg
    27121 tcgttgcagg ctatcgtcgg tgatcagccg taacgcctga ttcgggcaag cgcgaacgca
    27181 ggccggacct tctggcaagc aatcacacag gtcgcatttc accgcgacgg tctggacacc
    27241 cggttcccag cgcagcaatg catgttgggg cggcacgttt tcttcgccgt ctttgagtga
    27301 gccttcagcc tgaaaaacat attgcgcagg ggcattcacc ggacggcttc ctgaagcgct
    27361 gattgcgcca aatgggcaga ccacggcgca aagcttgcag ccaatacaga ggctttcgtt
    27421 gagttggatc gcatcatccc tctgggagat ggcattaacc gggcagacct gcaggcaagg
    27481 ggcttcctca cagtgatgac acacgacagg ggcagtgagt gttgacgtct tcgccagggc
    27541 caggcgcggg tgttgctgta aaccttgcgt tttatgcacg tccgaacagg cagcgagaca
    27601 ggtattacat cctgtacacc acagtggttc ggccaccaca aagcggttca tgtcggctcc
    27661 ttgaaaatga tatgaaacat tgcatttcat attgcataaa ggatgccatt ctgattaact
    27721 caatatattc aataagttat gtattgactg gattctgaac ggtacaaaaa tgatggtcat
    27781 taacgttcag caaaaaatgg attcgacacc cgtgtcgcag gggaaagcga gaatgtgatc
    27841 ttgatcacga gaagtgaggt atgctggccg caagcgcagc cagcacggaa tggagcaaag
    27901 taatcaggcg tgttctttca gccagttcag cacaacgtcg tggtgattgc tggttttgaa
    27961 atcgtcaaag acatgttcga ttttgccatc agcgtcaatc aggaagctga tgcgatgaat
    28021 gccatcgtag gttttgccca tgaaggactt ttcaccccag acgccgaatt gttcgcacac
    28081 ctggtggtcc tcatcagaca ggagcgtaaa gttaagcagc tctttttccg caaaacggga
    28141 gagtttttcg ggtttatcgg tgctgatacc cagcacatca acgcccgctt ttttcaactc
    28201 atccatgtta tcgcgtaagc cgcaggcctg tacggtacag ccgggggtca tggctttcgg
    28261 gtagaaataa accagaacac gctgtccctg gaagtcggtc aaattaactt gttctccgtc
    28321 ttgatccggc aagctaaatt tcggtgcgat atcaccggct ttcagtggat tcattactta
    28381 actccatcct gttcatcatg ttgggaataa ttgacgacgt taatactgcc ttgtgcattg
    28441 agttctgtac atagggcttt gaacgcttgc tcaatatttg ctgcgtccgc agatgcgggg
    28501 ctgtgggcgg ttatctgaat atgcaactgc gcagcccgtt cattttcagc aggttgcgtg
    28561 cgcgacacca gctccgcaat gttcatatga tgcgcgtcga aaagtgctgt gaagcgttca
    28621 attaaatgcg gggagtctgc cacatcgacc tgaacccaga cagatgctgg cattggcgga
    28681 cgcggacgcg ccgtcgtgcg cttcatcacg attaaaagat ccagttcggc acctttcaac
    28741 ggtaacgttg attcaatcag agtaatggca ttccatgaac cggaaagcag cataataaac
    28801 gtgaactctt ctcccagcat cgccaggcga ctgtcttcaa tattacagcc gcaactactg
    28861 acatgacggg tgatggtgtt cacaattcca gggcgatcgg cacccaacgc agtgatcacc
    28921 agataatgtt gcgatgacag tgtcaaactg gttattcctt taaggggtga gttgttctta
    28981 aggaaagcat aaaaaaaaca tgcatacaac aatcagaacg gttctgtctg cttgctttta
    29041 atgccatacc aaacgtacca ttgagacact tgtttgcaca gaggatggcc catgttcacg
    29101 ggaagtattg tcgcgattgt tactccgatg gatgaaaaag gtaatgtctg tcgggctagc
    29161 ttgaaaaaac tgattgatta tcatgtcgcc agcggtactt cggcgatcgt ttctgttggc
    29221 accactggcg agtccgctac cttaaatcat gacgaacatg ctgatgtggt gatgatgacg
    29281 ctggagctgg ctgatgggcg cattccggta attgccggga ccggcgctaa cgctactgcg
    29341 gaagccatta gcctgacgca gcgcttcaat gacagtggta tcgtcggctg cctgacggta
    29401 accccttact acaatcgtcc gtcgcaagaa ggtttgtatc agcatttcaa agccatcgct
    29461 gagcatactg acctgccgca aattctgtat aatgtgccgt cccgtactgg ctgcgatctg
    29521 ctcccggaaa cggtgggccg tctggcgaaa gtaaaaaata ttatcggaat caaagaggca
    29581 acagggaact taacgcgtgt aaaccagatc aaagagctgg tttcagatga ttttgttctg
    29641 ctgagcggcg atgatgcgag cgcgctggac ttcatgcaat tgggcggtca tggggttatt
    29701 tccgttacgg ctaacgtcgc agcgcgtgat atggcccaga tgtgcaaact ggcagcagaa
    29761 gggcattttg ccgaggcacg cgttattaat cagcgtctga tgccattaca caacaaacta
    29821 tttgtcgaac ccaatccaat cccggtgaaa tgggcatgta aggaactggg tcttgtggcg
    29881 accgatacgc tgcgcctgcc aatgacacca atcaccgaca gtggtcgtga gacggtcaga
    29941 gcggcgctta agcatgccgg tttgctgtaa agtttaggga gatttgatgg cttactctgt
    30001 tcaaaagtcg cgcctggcaa aggttgcggg tgtttcgctt gttttattac tcgctgcctg
    30061 tagttctgac tcacgctata agcgtcaggt cagtggtgat gaagcctacc tggaagcggc
    30121 accgcttgcg gagcttcatg ccccggctgg aatgattttg ccggtgacct ccggtgatta
    30181 tgcaatcccg gtgaccaacg gtagtggtgc tgtcggtaag gcgctggaca ttcgtccacc
    30241 agcccagccg ctggcactgg tttctggcgc gcgtacccag ttcacgggcg ataccgcttc
    30301 attgctggtg gaaaatggtc gtggcaatac tctgtggccg caggtggtta gcgtgctgca
    30361 ggcgaaaaac tacaccatca cccaacgtga tgatgctggt cagacactga ccaccgattg
    30421 ggtacaatgg aaccgtctgg acgaagacga gcagtatcgt ggtcgttatc aaatctctgt
    30481 taagccgcag ggttatcagc aggcggttac ggttaaactg ctgaacctgg aacaggcggg
    30541 caaaccggtt gcagacgcgg cttccatgca gcgttacagc acggagatga tgaacgttat
    30601 ttccgccggt ctggataaat ctgccactga cgccgcgaac gctgcgcaaa atcgtgcctc
    30661 caccactatg gacgtacaaa gtgcagctga tgacaccggt ttaccaatgc tggtcgtacg
    30721 cgggccgttc aatgtggttt ggcaacgtct gccagcggcg ctggaaaaag tgggcatgaa
    30781 agtgaccgac agcacccgtt cgcagggcaa catggccgta acttataagc cgctgtctga
    30841 cagcgactgg caggaactgg gcgcgagcga tccaggcctg gcatccggtg actataaact
    30901 gcaggttggc gatttagata accgcagcag cctacagttc atcgatccga aaggtcatac
    30961 tctgactcag agtcagaacg acgcgctggt agctgtcttc caggctgcgt ttagcaagta
    31021 aaaatacagg gctggaatca tccggccctt ttttctgata tgatacgcaa acgtgtgcgt
    31081 cggcaggaaa acgcgatttt agcggtaatt cgcacgaaat ttgtttgtcg gacgtagttc
    31141 ggataaggcg ttcacgccgc atccgacaaa acatccggca caccagacag caaaagattt
    31201 taaaacgtta attcacaccc aggagtgata aagatgcaaa agcaagctga gttgtatcgt
    31261 ggtaaagcga aaaccgtata cagcacggaa aacccggacc tgttggtgct cgaattccgc
    31321 aatgatacgt cagcagggga tggcgcgcgc attgagcagt ttgatcgcaa aggtatggtg
    31381 aacaacaagt tcaattactt cattatgagc aaactggctg aagcgggtat cccgactcaa
    31441 atggagcgtc tgctctccga taccgaatgt ctggtgaaaa agctggatat ggtgccggtt
    31501 gagtgtgtcg tgcgtaaccg tgctgctggc tctctggtga aacgtcttgg aatcgaagaa
    31561 ggtattgagc tgaacccgcc gctgttcgat ctgttcctga aaaacgacgc catgcacgat
    31621 ccgatggtca acgaatctta ctgcgaaacc tttggctggg tgagcaaaga gaacctggcg
    31681 cgtatgaaag agctgaccta caaagcgaac gacgtgctga aaaaactgtt cgatgatgct
    31741 ggtctgattc tggtcgactt caagctggag tttggcctgt acaaaggtga agtggtactg
    31801 ggcgatgagt tctccccgga cggcagccgc ctgtgggaca aagaaacact ggagaaaatg
    31861 gacaaagacc gtttccgcca gagcctcggt ggcctgatcg aagcctatga agccgtcgcc
    31921 cgccgcctgg gtgtacagct ggactgattt ttctggttgc ttgtttcttt agccggaggg
    31981 tactccggct aaatgtcttt gatacccccg ctacaatact tctcattatc tccctttgac
    32041 aggaaagtca taccaggcca atatgtatat acataacgta tatcccaaaa agggggggct
    32101 atggaaacta ctgtattttt aagtaatcgc agtcaggccg tcagattacc gaaagcggtc
    32161 gctctgccag aagacgttaa gaaagtagaa attattgcga ttggtcgtac aagaatcatc
    32221 actcccgccg gtgagtcctg ggacagttgg tttgatggcg aaaatgtgag tgcggatttt
    32281 atggatatcc gtgatcaacc cgcaatgcag gaaagggaat cattttgatg ctgaaattca
    32341 tgctggatac caatatctgt attttcacta ttaagaataa accagtacat gtcagagagc
    32401 ggtttaacct gaattctggc cggatgtgta taagttctgt aacattgatg gagcttatct
    32461 atggggcaga aaaaagtcag atgccagaac gcaatttggc ggttattgaa ggtttcgtat
    32521 ctcgtttggt agtgcttgat tatgatagtg ctgctgcgac gcataccggg caaatcaggg
    32581 ccgagcttgc ccggcaggga aggcctgttg gaccttttga ccagatgata gcgggacatg
    32641 ctcgcagtcg cggtttgatt gtggttacca acaatacccg agagtttgag cgagtggcgg
    32701 gaatcagact agaagactgg agctaacatt ctcttttact tctaacattc gtaaattata
    32761 taacgtggca tgaagcctta attcctccgc catttcatcc cgctttcctg tggtaatctt
    32821 cctctgaacc acctacgatc atttctataa tgaatatatt gttgaggtat ctatgcgttg
    32881 gcaagggcga cgtgaaagtg acaatgttga agacaggcgc aacagctccg gtggtccgtc
    32941 tatgggcggt cctggttttc gcctaccaag cggtaaaggc gggctgattt tactgttagt
    33001 cgtgctggtt gcaggctact atggtgttga tttaaccggg ttaatgactg gacagccggt
    33061 ttcccaacaa caatcaacgc ggtccattag ccctaatgaa gacgaagctg caaaattcac
    33121 ctcggtgatt ctggcaacca cggaagacac ctggggacaa cagttcgaga agatgggtaa
    33181 gacctatcag caaccgaagc tggtcatgta ccgtggaatg acgcgcaccg gctgcggggc
    33241 tggtcagtcc attatggggc cgttctattg cccggcggat ggcacagttt atatcgatct
    33301 ctccttctat gatgacatga aagacaaact tggcgcggat ggcgattttg cccaggggta
    33361 cgttatcgcc catgaagtcg gtcatcatgt gcagaaactg ttaggcatcg agccgaaagt
    33421 tcgtcaactg caacaaaacg cgacgcaggc ggaagtgaac cgcttatctg tgcgtatgga
    33481 actccaggcc gactgttttg ccggtgtctg ggggcataac atgcagcagc aaggcgttct
    33541 ggaaaccggc gatctggaag aggcgctgaa cgcggcgcag gccatcggcg atgaccgttt
    33601 acaacagcaa agtcaggggc gagtagtacc agatagtttc acacatggca cttctcagca
    33661 acgctacagc tggtttaaac gtggtttcga cagcggcgat ccggcacaat gcaatacttt
    33721 tggtaaaagc atttaacttc cggggcaggg atggctgaac tgactgcgct tcacacatta
    33781 acagcgcaaa tgaaacgtga agggatccgc cgcttgctgg tgttgagcgg ggaagagggt
    33841 tggtgttttg atcatgcgct taagttgcgt gatgccttac ctggcgactg gctgtggatt
    33901 tcgccgcagc cagatgctga aaaccactgt tcaccctcgg cgctacaaac tttacttggg
    33961 cgcgagttcc ggcatgcggt attcgacgcc cgccacggct ttgatgccgc tgcctttgca
    34021 gcacttagcg gaacgttgaa agcgggaagc tggctggttt tgttactccc tgtatgggaa
    34081 gagtgggaaa accaacctga tgccgactcg ctgcgctgga gtgattgccc tgaccctatt
    34141 gcgacgccgc attttgtcca gcatctcaaa cgcgtactta cggcggataa cgacgctatc
    34201 ctctggcggc aaaaccagcc gttctcgttg gcgcatttta ctccccgtac tgactggcac
    34261 cccgcgaccg gcgcaccaca accagaacaa cagcaactct tacagcagct actgaccatg
    34321 ccgccgggcg tggcagcggt aacggctgcg cgtgggcgcg gtaagtcggc gctggcaggg
    34381 caactcattt ctcgtattgc gggcagtgcg attgtcaccg cgcccgcaaa agcggcaacg
    34441 gatgtactgg cacaatttgc gggcgagaag tttcgcttta ttgcgccgga tgccttgtta
    34501 gccagcgatg agcaagccga ctggctggtg gtcgatgaag ccgcagccat acctgcgccg
    34561 ttgttgcatc aactggtatc gcgttttcct cgaacgttgt taaccactac ggtgcagggc
    34621 tacgaaggca ccggacgtgg ttttttgctg aaattttgcg ctcgctttcc gcatttacac
    34681 cattttgaac tgcaacagcc gatccgctgg gcgcagggat gcccgctgga aaaaatggtt
    34741 agtgaggcac tggtttttga cgatgaaaac ttcacccata caccacaagg caatattgtc
    34801 atttccgcat ttgaacagac gttatggcga agcgagccac aaacgccgtt aaaggtttat
    34861 cagctattgt ctggcgcgca ctaccggacc tcgccgctgg atttacgccg gatgatggat
    34921 gcaccagggc aacatttttt acaggcggct ggcgaaaacg agattgccgg agcgctgtgg
    34981 ctggtggatg aggggggatt atctcaacaa ctcagtcagg cggtatgggc aggttttcgt
    35041 cgcccgcggg gtaatctggt ggcccagtcg ctggcggcgc acggcagcaa tccactggcg
    35101 gcgacattgc gcggacggcg ggtcagccgg atcgcagttc atccggcgcg tcagcgcgaa
    35161 ggcgttgggc aacagctcat tgccagcgct ttgcaatata ggcctggcct cgactatctt
    35221 tcggtgagtt ttggttacac cggggagtta tggcgtttct ggcaacgctg cggttttgtg
    35281 ctggtgcgaa tgggtaatca tcgtgaagcc agcagcggtt gctatacggc gatggcgctg
    35341 ttaccgatga gtgatgcggg taaacagctg gctgaacgtg agcattaccg tttacgtcgc
    35401 gatgcgcaag ctctcgcgca gtggaatggc gaaacacttc ctgttgatcc actaaacgat
    35461 gccatccttt ctgacgacga ctggcttgaa ctggccggtt ttgcttttgc tcatcgtccg
    35521 ctattaactt cgttaggttg cttaatgcgt ctgttacaaa ccagcgaact ggcattaccg
    35581 gcgctgcgtg ggcgtttaca gaaaaacgtc agtgacgcgc agttatgtac cacacttaaa
    35641 ctttcaggcc gcaagatgtt actggtccgt cagcgggaag aggccgcgca ggcgctgttc
    35701 gcacttaatg atgttcgcac tgagcgtctg cgcgatcgca taacgcaatg gcaatttttt
    35761 cactgactcc ttcaattttc tcccatggtc attgtgagtc agcggcgtaa aattaatgac
    35821 atcagttaag gagttcgcca tgaaacatga ccattttgtt gttcaaagcc cggataaacc
    35881 tgcgcaacag ttgttactgc ttttccacgg tgtcggggat aacccggtgg caatggggga
    35941 aataggtagc tggtttgcac cgctgtttcc tgatgcgctg gtggtgagtg tcggtggcgc
    36001 ggagccgagc ggtaatccgg cggggcgtca gtggttttcg gtgcagggta ttacggaaga
    36061 taatcgccag gcgcgtgtgg atgcgatcat gccgacgttt attgaaacgg tgcgctactg
    36121 gcagaaacag agcggagtag gggctaacgc cacggcgctc atcggtttct ctcaaggcgc
    36181 gattatggtg ctggagagca ttaaagccga accgggcctt gcttcacgcg tcattgcttt
    36241 taacggacgc tatgcgagcc tgccggaaac tgcgtctaca gccacgacaa ttcacctgat
    36301 tcatggcggt gaagatccgg ttatcgatct ggcgcatgcg gttgcggcgc aggaagcgtt
    36361 aatcagtgcc ggtggtgacg tgacgctgga tatcgtggag gatttaggcc atgcgatcga
    36421 caatcgcagt atgcaatttg ccctggatca cttgcgttat accattccga aacattattt
    36481 cgacgaagcg ctaagtggcg gtaagcctgg cgatgatgat gtgattgaaa tgatgtaaaa
    36541 gacagattac ctggcctgat aagacgcgtg agcgtcgcat caggcatcgt tcaccattgc
    36601 cggatgcggc gttaacgcct tatccggcct acggttgagc actcagctta cttcttcggt
    36661 tgatcctttt tcggccagtc gtcgtcatcg tcccacttat cgttgaaatc acgatgcggg
    36721 ggaagctccg gcttgttggc gagaaatttc ttatggtcca cgcgcttgag atctttaatc
    36781 acgttcagca ggacgcctac caaaaagaca atcaccagaa tccaccaata tttagccagc
    36841 cagtccatgc ttatttcctc ttgcagaacc actcatcagg cgacgagctg ttccatgata
    36901 cgttgataca tacgggcaag tagctgcagg tcggcagcgt tcacacattc attaatttta
    36961 tgaatagtgg cattgaccgg cccgagttcc accacctgcg cccccatgcg agcaataaag
    37021 cgcccgtcgg acgttccgcc tgtggtcagt agctgcggtt taatttcatt atagtgctca
    37081 accgcgttaa cgaccgcatc caccagttta ccgcgcgcgg tcaaaaatgg ctgcccggaa
    37141 agccaccaat ccaccgtata gcgcagttga tgtttttcaa gcagggcaag cacctgcgct
    37201 ttgatcatct catcagtcag ttcggtgctg aagcggaagt taaactgcac aaacagttca
    37261 cccggaataa cgttgttact gcccgttccc gcctgaatat tagcaatctg catactggtc
    37321 gccgggaaaa attcattgcc ctgatcccac tcaatagcca ctaattcatt aaggaaaggt
    37381 gctgcgcgat gtactggatt gtcagccaga tgcgggtagg ccacatgccc ctgaacgcca
    37441 tgaatggtaa ggttgcaggt taacgatccg cgacgaccat ttttcaccac atcaccaacc
    37501 acttcgatac tcgacggttc gccgaccagg cagtaatcga gacgctcatt acgtgccatc
    37561 aacgcttcga cgacttttac cgtaccgttg tgggcactgg cttcttcatc agaggtgatc
    37621 agaaatgcca gtcgccccgt atggttggga tgttgtgcga caaagcgttc tgccgctacc
    37681 accatcgccg ccagcgagcc tttcatatct gccgcaccgc gtccgaataa catgccatca
    37741 cgaatggtgg gttcaaacgg cggattgatc caacgatcgg cgtcgccagg cggcaccacg
    37801 tcggtatgcc cggcaaaggc caacgtttca ccttgcccac gccatgccca aaaattctgc
    37861 gtatcggcaa aatccatgcg ttcaacggta aaaccgatcg cctgcaaacg ttcaatcaac
    37921 aaagcctggc atcctgcgtc atcaggactc agggaagggc ggcgaataag ctgttgtgtc
    37981 agctcaataa ccgggcacga catagactac acctcatgga aaaattgctg ataactggaa
    38041 tcactgaaac ccagcagcat aggcttacca ggcgcgcaga gcaatggacg tttgataatt
    38101 gcaggcattt cagtcattaa tgccgccgca gaggccgcat cggtgatttt attgcgggtg
    38161 gtttcgtcca gtttacgcca ggttgtacca cgggtgttca gtaacgcttc ccagcctaat
    38221 tcgttgataa aaccgttcaa taattcgctg tccagcccat cgacgcggta atcatgaaaa
    38281 cgatagtcga tgttattggc ttccagccaa cggcgagcct ttttaatggt gtcacaattt
    38341 ttgatgccgt aaagtgtaac cataatgaat cctttttcga tctatgagaa taaatactca
    38401 tttaagggtg aaa
//
LOCUS       DAFNBJ010000024        37940 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000024 DAFNBJ010000000
VERSION     DAFNBJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.024"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(112..399)
                     /locus_tag="L2X49_001246"
     CDS             complement(112..399)
                     /locus_tag="L2X49_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBN4796358.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(474..617)
                     /gene="mgrB"
                     /locus_tag="L2X49_001247"
     CDS             complement(474..617)
                     /gene="mgrB"
                     /locus_tag="L2X49_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBN4796359.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            776..1015
                     /locus_tag="L2X49_001248"
     CDS             776..1015
                     /locus_tag="L2X49_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796360.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1158..1949)
                     /gene="kdgR"
                     /locus_tag="L2X49_001249"
     CDS             complement(1158..1949)
                     /gene="kdgR"
                     /locus_tag="L2X49_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBN4796361.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMRELSR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTEQTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2126..3499
                     /locus_tag="L2X49_001250"
     CDS             2126..3499
                     /locus_tag="L2X49_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4796362.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3545..4426)
                     /gene="htpX"
                     /locus_tag="L2X49_001251"
     CDS             complement(3545..4426)
                     /gene="htpX"
                     /locus_tag="L2X49_001251"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBN4796363.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4618..6666)
                     /gene="prc"
                     /locus_tag="L2X49_001252"
     CDS             complement(4618..6666)
                     /gene="prc"
                     /locus_tag="L2X49_001252"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBN4796364.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6686..7384)
                     /gene="proQ"
                     /locus_tag="L2X49_001253"
     CDS             complement(6686..7384)
                     /gene="proQ"
                     /locus_tag="L2X49_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBN4796365.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7481..7978)
                     /gene="msrC"
                     /locus_tag="L2X49_001254"
     CDS             complement(7481..7978)
                     /gene="msrC"
                     /locus_tag="L2X49_001254"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBN4796366.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVNNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8108..9391
                     /gene="yebS"
                     /locus_tag="L2X49_001255"
     CDS             8108..9391
                     /gene="yebS"
                     /locus_tag="L2X49_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBN4796367.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9360..11993
                     /gene="yebT"
                     /locus_tag="L2X49_001256"
     CDS             9360..11993
                     /gene="yebT"
                     /locus_tag="L2X49_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HBN4796368.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGLVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12073..13512
                     /gene="rsmF"
                     /locus_tag="L2X49_001257"
     CDS             12073..13512
                     /gene="rsmF"
                     /locus_tag="L2X49_001257"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBN4796369.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAATGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13630..13866
                     /locus_tag="L2X49_001258"
     CDS             13630..13866
                     /locus_tag="L2X49_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBN4796370.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13971..14162
                     /locus_tag="L2X49_001259"
     CDS             13971..14162
                     /locus_tag="L2X49_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBN4796371.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14163..14819)
                     /gene="pphA"
                     /locus_tag="L2X49_001260"
     CDS             complement(14163..14819)
                     /gene="pphA"
                     /locus_tag="L2X49_001260"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBN4796372.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15215..15556)
                     /locus_tag="L2X49_001261"
     CDS             complement(15215..15556)
                     /locus_tag="L2X49_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBN4796373.1"
                     /translation="MMKKSILPFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15569..16441)
                     /gene="copD"
                     /locus_tag="L2X49_001262"
     CDS             complement(15569..16441)
                     /gene="copD"
                     /locus_tag="L2X49_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HBN4796374.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIDAV
                     VLVIISLLATLEPF"
     gene            complement(16445..16819)
                     /gene="yobA"
                     /locus_tag="L2X49_001263"
     CDS             complement(16445..16819)
                     /gene="yobA"
                     /locus_tag="L2X49_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBN4796375.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16958..17188
                     /gene="holE"
                     /locus_tag="L2X49_001264"
     CDS             16958..17188
                     /gene="holE"
                     /locus_tag="L2X49_001264"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBN4796376.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17290..17946
                     /locus_tag="L2X49_001265"
     CDS             17290..17946
                     /locus_tag="L2X49_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBN4796377.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17970..18632
                     /gene="exoX"
                     /locus_tag="L2X49_001266"
     CDS             17970..18632
                     /gene="exoX"
                     /locus_tag="L2X49_001266"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBN4796378.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18629..20689)
                     /gene="ptrB"
                     /locus_tag="L2X49_001267"
     CDS             complement(18629..20689)
                     /gene="ptrB"
                     /locus_tag="L2X49_001267"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBN4796379.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20898..21557)
                     /locus_tag="L2X49_001268"
     CDS             complement(20898..21557)
                     /locus_tag="L2X49_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBN4796380.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21884..22240)
                     /locus_tag="L2X49_001269"
     CDS             complement(21884..22240)
                     /locus_tag="L2X49_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBN4796381.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22307..22597)
                     /gene="yebG"
                     /locus_tag="L2X49_001270"
     CDS             complement(22307..22597)
                     /gene="yebG"
                     /locus_tag="L2X49_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HBN4796382.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22731..23909
                     /gene="purT"
                     /locus_tag="L2X49_001271"
     CDS             22731..23909
                     /gene="purT"
                     /locus_tag="L2X49_001271"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBN4796383.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(23965..24606)
                     /gene="kdgA"
                     /locus_tag="L2X49_001272"
                     /gene_synonym="eda"
     CDS             complement(23965..24606)
                     /gene="kdgA"
                     /locus_tag="L2X49_001272"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBN4796384.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24643..26454)
                     /gene="edd"
                     /locus_tag="L2X49_001273"
     CDS             complement(24643..26454)
                     /gene="edd"
                     /locus_tag="L2X49_001273"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBN4796385.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26689..28164)
                     /gene="zwf"
                     /locus_tag="L2X49_001274"
     CDS             complement(26689..28164)
                     /gene="zwf"
                     /locus_tag="L2X49_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBN4796386.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28502..29371
                     /gene="hexR"
                     /locus_tag="L2X49_001275"
                     /gene_synonym="yebK"
     CDS             28502..29371
                     /gene="hexR"
                     /locus_tag="L2X49_001275"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBN4796387.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29499..30941
                     /gene="pyk"
                     /locus_tag="L2X49_001276"
     CDS             29499..30941
                     /gene="pyk"
                     /locus_tag="L2X49_001276"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBN4796388.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAARLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31072..32043)
                     /gene="lpxM"
                     /locus_tag="L2X49_001277"
                     /gene_synonym="msbB"
     CDS             complement(31072..32043)
                     /gene="lpxM"
                     /locus_tag="L2X49_001277"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBN4796389.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32163..33485)
                     /gene="mepM"
                     /locus_tag="L2X49_001278"
     CDS             complement(32163..33485)
                     /gene="mepM"
                     /locus_tag="L2X49_001278"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBN4796390.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33501..34433)
                     /gene="znuA"
                     /locus_tag="L2X49_001279"
     CDS             complement(33501..34433)
                     /gene="znuA"
                     /locus_tag="L2X49_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBN4796391.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34512..35267
                     /gene="znuC"
                     /locus_tag="L2X49_001280"
     CDS             34512..35267
                     /gene="znuC"
                     /locus_tag="L2X49_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBN4796392.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35264..36049
                     /gene="znuB"
                     /locus_tag="L2X49_001281"
     CDS             35264..36049
                     /gene="znuB"
                     /locus_tag="L2X49_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBN4796393.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36196..37206)
                     /gene="ruvB"
                     /locus_tag="L2X49_001282"
     CDS             complement(36196..37206)
                     /gene="ruvB"
                     /locus_tag="L2X49_001282"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBN4796394.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37215..37826)
                     /gene="ruvA"
                     /locus_tag="L2X49_001283"
     CDS             complement(37215..37826)
                     /gene="ruvA"
                     /locus_tag="L2X49_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBN4796395.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9083 a         9775 c         9361 g         9721 t
ORIGIN      
        1 ttgaagtcag accagagttt taatttgaag cagttgcacc atggcagtgc gtataatcag
       61 gatgaattga gtaacccttc aacgacgggg ttactcaaga ttgaggaagg attatcgttc
      121 ggcgaccaga cgaataatat cttcgtcttc aaccgatttt tgactctcaa cggtcttttt
      181 atctgctttc tcaggttcgt ttgcttcgca caaacgacgt aacagtgcat tttgacgttt
      241 ttgctgatcc agcaacgcct caagcagttc aatctgttcg ttagtccgag aactggcacg
      301 attgataaaa aaccacaaga tgagcccgat aagaagaacc accaccgata caaccaaaga
      361 cgcaatgttc atcacgcctg aatttacaac ttcgttcatt tcaccacctc tgagtagagg
      421 cgctattcta ccactgctgg agaggaagaa aatctagtgc tgaaaaaatg atatcaccac
      481 gggataaact ggttaatggc acaaattccg ctgaaaaatt gtacatcctg atcgcacatc
      541 atgttgaata cctgcgccca aagcagcaag caagccaaca ccacgacaac cagaacgacc
      601 catcgaaact ttttcactcc aatctccgtt tcacctacct tatgtcatta aactagcatg
      661 cgttagttaa acagcgccta actatgtcga tattcgtgct ttttcggaat gagtcgcttg
      721 cttcagcgca tattgccgct acgattaagc gaacataaaa agagaagagg ttgtaatgcg
      781 attcatcatt cgcacagtta tgctgatagc actggtatgg ataggattat tactcagcgg
      841 atatggcgtt ttgattggaa gcaaagagaa tgccgcagga ttaggtttgc aatgtaccta
      901 tctgactgcc agaggcacca gcacggtaca atacttgcac actaaaagtg gtttcttggg
      961 gataacagat tgcccactgt tacgtaaaag caatatcgtg gtcgataatg gttaagggta
     1021 ataaataacc agattctcac tcatgagtaa aatattctta tagcaagcgc gtttaatata
     1081 acgcaccttc aactttattt ttctcatgct aaataaagaa cctgtctgtt tgatattccg
     1141 acaggttctt actctcatca gaacggatag tcgtgataac ccatttgggc agaaattttg
     1201 cgcgctgcgg tatgcaacat tgcgacatat tcctgtaaac gctcttcaga gaaacgcaac
     1261 gtcgggaagg agatgctcaa accggcaatg accacgccaa agcgatcaaa taccggtacc
     1321 gcaatgcatc gcagcccttc ttcctgctct tcgttatctt cgccataccc ctgctcgcgc
     1381 acctggtcca gaacgggtaa taacgcttct gtactggtga tggtctgctc ggtactgcgt
     1441 ttatactcca cgccctcaag aatttgcttc acttcatcga gatcgcgcca tgccagcagt
     1501 accttaccaa tcgcggtgct gtacagcgga ttacgacgcc caatccgtga atacatgcgc
     1561 aaattgtaca tagagtcaat tttgtgaatg taaacaatac tgtcttcgtc cagtgcgccg
     1621 aggtggatag tttctttggt caggcgggag agctcacgca tctggatatc tgcgctacga
     1681 attaaatcga cgttttgtaa cgcgcgagcg cccagttcaa acaatttcag ggtcagggaa
     1741 tatttctccg actccccttc ctgcgccaca taacctaagg ttttcatggt ctgtaaaaag
     1801 cgataaacgg tgctttttga catcatgacg cgctgcgaca gctcggttat ccctatttcg
     1861 cgctcttcac ccagcgcctg caaaatgcca aaaactttta gcacggaaga tacagaatca
     1921 ggctgtttat ccagatctgc gttagccatt tatcacctca ttgcgagtgt tttataaaaa
     1981 tcagaactgt tttttattat aatttcgcac cagggtggtc gcaatccatc ttttgccggt
     2041 tagttacaat tctgcgacat ccaccgtgaa tatcagtgct agaatcatac ccctgttgat
     2101 tattcaccaa agatataaaa ttcctatgcc aaaagttcag gccgacggcc tgccattgcc
     2161 ccagcgatac ggtgcgatat taaccattgt gattggcatt tcgatggctg tccttgacgg
     2221 cgcaatcgcc aacgtcgccc tgccaacaat cgccacggac cttcatgcca cgccagccag
     2281 ttccatctgg gtagtgaacg cctatcaaat cgccattgtc atctccctgc tctcgttttc
     2341 gtttctgggc gatatgtttg gctatcgacg tatttataaa tgcggtctgg tcgtttttct
     2401 gttgtcttca ctgttctgcg ccctttctga ttcgctgcaa atgctcaccc ttgcgcgtgt
     2461 catacaaggt ttcggcggtg cagcgttgat gagcgttaat accgcactta tccgcctgat
     2521 ctatccacaa cgttttctgg ggagagggat gggcataaac tcgtttattg ttgccgtctc
     2581 ttctgctgcc gggccgacaa ttgctgcagc aatcctctcc atcgcatcct ggaaatggtt
     2641 atttttaatc aacgtaccgt taggtattat cgccctgctt ctggcgatgc gttttctgcc
     2701 acccaatggt tctcgcgcca gtaaaccccg tttcgacctg cccagcgccg tgatgaacgc
     2761 gttaaccttc ggcctgctta tcactgcgtt gagtggtttc gctcaggggc aatcgctgac
     2821 gttaattgct gcggaactgg tggtaatggt tgttgttggt attttcttta ttcgccgcca
     2881 gctttctctt cccgtaccgc tgctaccggt ggatttactg cgtatcccgc tgttttcact
     2941 ttctatttgc acatctgttt gctctttctg cgcacaaatg ctggcaatgg tttccctgcc
     3001 cttttacctg caaaccgtgc tcgggcgtag tgaagtcgaa acaggtttac ttctgacacc
     3061 gtggccgtta gcaacgatgg tgatggctcc gctggcaggc tatttgattg aacgcgtaca
     3121 tgcaggattg ctgggtgctt tagggttgtt catcatggct gcggggcttt tttccctggt
     3181 tctgctgccc gcgtcacctg cggatatcaa tattatctgg ccgatgatct tatgtggcgc
     3241 tggatttggc ttattccagt cacccaataa ccacaccatt attacctccg ctccgcgcga
     3301 acgtagcggt ggggccagtg gcatgttagg aacggctcgt ctactgggtc agagtagtgg
     3361 cgcggcgctg gtggcgctga tgctaaatca gttcggtgat aatggtacgc acgtttcgct
     3421 gatggctgcg gctattctgg cagtgattgc tgcctgtgtg agtggtttac gtatcactca
     3481 gccacgatcc agggcataaa aaaagcgcgt cgatcaggac gcgcttttta gtatttactt
     3541 catattactt caggtattca cccgtacgca gagcttcaat tcgtttatcc agcggcgggt
     3601 gagtcatgaa caactcactg agcgatttcg acttaccgtt aatgcagaaa gccatcatgc
     3661 tggttgcttc ttgcggttca tagctggttt tcaggcgctg cagcgcggca atcattttct
     3721 cgcgaccaac cagttttgcc gaaccggcat cagcatggaa ttcacgatga cgcgagaacc
     3781 acatggtgat aatgctcgca agaataccaa acaccagttc cagaaccgtt gcaaccgcaa
     3841 agtagatcag cgggttgccg ttgctctctt caccttcatc acgatttccg cccataaaac
     3901 ccgcggcaag ctgcgccaga atacgggaaa taaagataac gaaggtgttc accacgccct
     3961 gaatcagcgt catggtgacc atatcaccat tggcgatgtg gctgatttcg tgagcaatta
     4021 ccgcctcggc ttcatccggg ctcatgttct gcagcaaacc ggtgctgaca gcaaccagag
     4081 aggcatcacg gcgcgcaccg gttgcaaaag cgttgatgtc cggcgcatgg tagatagcca
     4141 cttgcggcat agcgatccct gcctgacgag cctgggttgc tacagtatta accagccaac
     4201 gttccctttc gttacgcggt tgctcgatca cttccccgcc aacagatcgt aatgccatcc
     4261 atttggacat cagaagcgaa acgaaggaac caccaaaacc gaacagcaag gccatgatca
     4321 tcagcccctg aacgctgctc gactgtatcc ctgtcaggct cagtaccagc ccgaaaacga
     4381 ccattacggc caggttcgtt agcaggaaga gcgcgattcg catcataatt ttctttttac
     4441 ctcagtttaa caaaacgcaa tatgcgatac ccacatcgta tgggttacgc gactattttc
     4501 aagtctggat agtgcgtaag tcaccagaaa gacacaactt tacattttgt agcatctgat
     4561 ttacggcatc ttgccgctgt taaaaaatca ggcacaattt cttgtgcctg attgatatta
     4621 cttgacggga gcgggttgtt ccgcgggtct ggctttttca agcttcgcca gatcgagtgc
     4681 gatattcacc gtctcatcca gataaggatc cggctcctgg taatctttag gtagatcatc
     4741 cagcttcttc aactccggtt taccttcgcg tttaaagcgt tcgttcaaac gcgccagacg
     4801 cgtcgcatca tcttcattat tctctttctc acgcacagcg tagttcagag aaacgatatt
     4861 gcgcttgtcc ttcatagcgt tgaagcgcgc gatatccttc atgatgttct ggaactcagg
     4921 atctttcgcg atacgcgcat tatgttcctt cagcagctcc ggcccaaagg ccgttaaatc
     4981 gcctgatttc acataagtcg cggcatcaat gctatcccac ggcagcgcgt tatcttcgaa
     5041 tttctcaccc gtttccgttt cttcattacc cgtcggcatg atgatgtctg gcgttacgcc
     5101 tttacgttgc gtactgccgc cgttaacgcg atagaatttc tggatcgtgt actgcacaga
     5161 acccagcgct ggccattcag gacgtaacat ctgatcgtaa atacggttca atgaacggta
     5221 ttgctgaacg gtgcctttac caaacgtcgg ttcacccaca accagcgcac gaccgtaatc
     5281 ctgcattgcc gcggcaaaga tttctgaagc cgaagcactg aaacggtcaa ccagtaccac
     5341 cagcgggcct ttatagaaca cctgcccgtc ggtatcgctg tcttcacgaa ccttgccgtt
     5401 gttatcgcgg acctgaacaa tgggacccgc aggaataaac agacctgaga gcgataccgc
     5461 ttcggtcagc gccccaccgc cattgctacg caggtcgatg atcacactgc tgacattctg
     5521 tttttccagt ttctgcagtt gaactttgac atcgtctgtc aaacccacat agaagcccgg
     5581 aatatccagc acgccgactt tctctttacc gacggtcttc accgacattt taaccgcgcg
     5641 gtcttcgaga cgaatacgtt cacgggtcaa tgttacagtg cgggtcttgg tccctttacc
     5701 agcaggtaaa atttccagac gaactttact gcccttcggc cctttaatta aggcaaccac
     5761 atcatcaaga cgccagccaa tcacgtcaac catcggcttg cctgtttgac caacaccgac
     5821 aattttgtca ccaacgctga tagctttact cttcgctgcc ggaccacctg ccaccatcga
     5881 attgataacg gtgtagtcat catccatttg cagcactgcg ccaatacctt ccagcgacaa
     5941 actcatttca gtgttgaact gttcggtatt acgcggggaa agatagttgg tatgcgggtc
     6001 gatttcacgc gcaaacgccg tcattgccag cgagaaaaca tcttcgctgt tggtttgcgc
     6061 cagacgacga atggcaaatt tgtagcggcg tgtcagggtt tcacgaattt ctttatccgt
     6121 ttttcctgcc agcttcaggc ttaactcgtc gaatttgact ttactgtccc acagcgcgtt
     6181 caactcagcc tcgtttttcg gccagggcgc tttgctgcgg tcaaggttat aagtgtcgtt
     6241 gccggtgaaa tccatcggct tttccagtac cgacaaagcg tactggtaac gctcaaaacg
     6301 gcgcttttgc gccagattgt agagatcgta gaaaacgtcg agtttgcctg aacgcagttc
     6361 atcgcctaac tcggttttct ttttcgcgaa ctgttcaaca tcgcttgcca gcagcacgtt
     6421 gtggctgtaa tcgagcagat tcaggtagcg gtcaaagatt ttggccgaaa atgcctgatc
     6481 gaggtcgaac tggcgataat gagaacgggt gaagcgcgac gttacgcgct cacttaccgt
     6541 cgcatgctgc gtctcttcct ttaataccgg aatttgatca gcacgcgtga tatcttctac
     6601 agcgaaggtc tggcctgcta ttgcaagcag gccagctaac gcggtaagcc taaaaaacat
     6661 gttcatgcct ggcccggcct ccgtttcaga acaccaggtg ttctgcgcgc acaatcaaag
     6721 acatacccga attcagctgg acgcggacgc cgtctttggt gatttctaat acggtggcat
     6781 ccatcgcgtt ttgacccgct ttcaccttca gagcttgtcc gacagtcaga gctgaaatgt
     6841 cagaaaccgg ggtgtgctgt tcttcgcgag gtgcttttac tgtttttggc gctttctcta
     6901 ccggcttttg cgcacgaggt ttacgttcag cgccttcttt gcggcgtggc gtggtcggac
     6961 gtggcttgcg ttcgcggcgc ggtgcgtctt ctttctcacc agcagctgcg gcagcttcgc
     7021 gttttttcgc ttgctgttca gcacgctgtg cctgaacacg cgctttcgct tcttcaagct
     7081 gcttgcgagc atgctctaca tgttgctcgt ccagctcacc gcatgggttg ccgtcaagat
     7141 cgacacgcgt tgcgccgggt ttaacaccgt aaagataacg ccagctcgaa gtgtagagac
     7201 gtaaagcgga tcgtaattgc gttttgctca ggttcatttc cccggcaaca cgatcgacca
     7261 aatcctgaaa aataccgatt ttcagcggac gcgcttcacc ttccgcactg aaacagtggg
     7321 gaaaacgttc ggccagaaac gcgattactt ctttactgct attcaactta ggttgatttt
     7381 ccatgaaatt tcctgattac aacggacgta gccaacaagc gcaggcatga acaggcgtca
     7441 ttataatgac gctaccagta aatgctacgt tatccgttga ttatcctgcg acgctcgcaa
     7501 agaatttttt gtaatccgtc gttgcaagca ctttttcaag ctgtgccaca agctgacgta
     7561 agccttgctc gtcctcgtct gtaaagcgac cgaagacggt actatcgatg tcaagaacac
     7621 caataatctg attattcacc accagcggca gaacaatttc agaattactc gccgcatcac
     7681 aggcaatatg cccgtcaaac gcgtgaacat cctcgatacg ctgcacttga ttgcgggcaa
     7741 ccgcagtgcc gcacaccccg cgcccgacgg gtatccggac acaggcaatt ttgccctgaa
     7801 atggtccgag taccagtgta tcgtcctcaa gcaaataaaa acctgcccag tttacgtcag
     7861 tgagacgctc gtataacaac gcactggtgt tcgcaagcgt tgccagaaaa ctggtttctc
     7921 ccgccatcag cgcgttaaag tcgcgattta aatccgcgta aaattctgtt ttgttcatta
     7981 tataatcact tggttgtctt acctggaact gccagcctat taaaataagc attaaatgcg
     8041 ttaatgctca agatcattcc aatcatgggt taatattaat gttaattctt attacatttg
     8101 gcacgtcatg gctcttaaca caccacaaat tacgccgaca aaaaagataa cagtgagggc
     8161 aatcggcgag gaactgccgc gtggtgatta ccaacgttgc ccgcaatgtg acatgctgtt
     8221 tagcctgccc gagataaatt ctcatcagag tgcctattgt ccgcgctgtc aggcaaaaat
     8281 tcgcgacggg cgcgactggt cgctaacgcg cctggcggca atggctttca ccatgctgtt
     8341 gctgatgccg tttgcctggg gcgaaccgct gttgcatatc tggctgttag gcattcgtat
     8401 tgacgccaac gttatgcaag gcatctggca aatgaccaaa cagggcgatg cgataacggg
     8461 gtcgatggtc tttttctgcg ttatcggtgc ccccctcatt ctggtgacct ccatagctta
     8521 tttatggttt ggtaaccgac tgggaatgaa tctacgcccg gtactgctga tgcttgagcg
     8581 acttaaagag tgggtaatgc tggatatcta cctggtcggc attggcgttg cttctataaa
     8641 ggtacaggat tatgcccata tccaggcggg tgtcggcttg ttctcttttg tggcgttggt
     8701 gattttaacg acggtgacgt tgtcacatct taatgtcgag gagctgtggg agcgatttta
     8761 tccgcagcgc cccgctacgc gtagggacga gaaacttcgt gtctgtcttg ggtgccattt
     8821 taccggctat cctgatcagc gtggtcgctg cccgcgttgc catatcccgc tacgcctgcg
     8881 tcgccgtcat agtttgcaaa aatgctgggc ggcgctgtta gcgtcaatcg ttttgttgtt
     8941 acctgccaac ctgttgccta tttctatcat ttatctgaac ggaggacggc aggaagatac
     9001 aattctttcc ggaattatgt cgctggcaag tagcaacatt gcggttgcgg gaatcgtgtt
     9061 tatcgccagt attctggtac cgtttactaa agtgatcgtc atgttcactt tactgttgag
     9121 cattcatttt aaatgccagc aaggtttacg cacacgcatt ctgttactgc ggatggtgac
     9181 ctggattggt cgctggtcga tgctcgacct gtttgtcata tctttaacca tgtcgctgat
     9241 taatcgcgat cagatcctcg cttttactat gggaccggct gcgttttatt tcggcgcagc
     9301 ggtaattttg actattcttg ctgtggaatg gctggacagc cgcttacttt gggatgcaca
     9361 tgagtcagga aacgcccgct tcgacgactg aagcgcagat taaaaataaa cgccgtatct
     9421 cacctttctg gctgctgcct ttcatcgcgc taatgattgc cggttggctg atttgggaca
     9481 gttatcagga ccggggtaat accgtcacca tcgactttat gtcggcggat ggtattgttc
     9541 cgggccgtac gcctgttcgt tatcagggcg ttgaagtcgg aacagtgcag gatatcagcc
     9601 tcagcgacga tcttcgtaag attgaagtca aggtcagcat caagtccgat atgaaagatg
     9661 cgctgcgcga agagactcag ttctggctgg tgacgccaaa agcatcgttg gcaggtgtct
     9721 ccgggctgga cgccctcgtc ggtggtaact atatcggcat gatgccaggt aaaggtaaag
     9781 agcaggatca ctttgtcgca ctcgataccc aaccgaaata tcgactggac aatggcgatc
     9841 tgatgatcca cctgcaagcc cccgatctcg gttcgctgaa cagcggttca ttggtctatt
     9901 tccgcaagat ccctgtggga aaagtctacg actatgccat caatcccaac aagcaaggcg
     9961 tggtgattga tgtcctgatc gagcggcgtt ttaccgatct ggtgaaaaaa ggtagccgtt
    10021 tctggaacgt ttccggcgtt gatgccaatg tcagtatcag tggcgcgaag gtgaaactgg
    10081 aaagtctggc ggcactggtt aacggtgcga ttgccttcga ttcacctgaa gagtcgaaac
    10141 ctgccgaggc ggaagatacc tttggtctgt atgaagatct ggcccacagc cagcgtggcg
    10201 taataataaa actggaactg ccgagtgggg caggattaac cgccgactcg acgccattaa
    10261 tgtatcaggg actggaagtc ggacagctaa ctaaactgga tttaaatcct ggtggtaaag
    10321 tcactgggga aatgacagtt gatcccagcg tcgttaccct gcttcgtgaa aatacccgca
    10381 tcgaattacg taatccgaaa ttatccctta gcgatgccaa tctcagcgcc ctgctgactg
    10441 gcaaaacctt cgagttggta cccggcgatg gcgagccacg caaagagttc gttgttgtgc
    10501 caggcgaaaa agcactgctg catgaacctg atgttctgac gctgaccctg accgcgccgg
    10561 aaagttacgg tattgatgcg ggtcagccgc tcattcttca cggcgtgcag gtaggcctgg
    10621 ttatcgatcg taaactcacc agcaaaggcg tcacctttac cgtcgccatc gagcctcagc
    10681 atcgagaact ggtaaaaggc gatagcaaat ttgtcgtcaa cagccgtgtc gacgtgaagg
    10741 tggggctgga tggcgttgag tttctcggtg ccagcgcctc agaatggatt aacggcggga
    10801 tacgtattct gccgggcgat aaaggcgaga tgaaagccag ctatccactg tatgccaatc
    10861 tggaaaaagc gctggagaac agccttagcg atttacccac cacaaccgtg agtttgagtg
    10921 cagagacgct gccggatgtg caggcaggat cggtagtgct ctaccgtaaa tttgaagttg
    10981 gtgaagttat taccgtccgt ccgcgagcta acgcgtttga tatcgatctg catattaagc
    11041 cggagtatcg caaccttctg accagcaata gcgtgttctg ggcagaaggc ggggcgaaag
    11101 ttcagctgaa tggtagtggc ctgaccgtac aggcatcccc gctctccaga gcattaaagg
    11161 gagccattag cttcgataac ctcagcggtg ccagcgccag tcagcgtaaa ggcgacaaac
    11221 gaattctgta tgcttccgaa acagcggccc gtgcggttgg tgggcagatt acgcttcacg
    11281 ctttcgatgc cggaaaactg gcggtcggga tgccaattcg ctatctcggt attgatatcg
    11341 ggcaaatcca gacgctggat ctgattaccg cgcgcaatga agtacaggca aaggcggtgc
    11401 tctatccgga gtatgtccag accttcgccc gcggcggtac gcgcttctcg gtggtcacac
    11461 cgcaaatttc agccgcgggc gttgagcatc tagatactat cctccagccg tatatcaacg
    11521 tcgaaccagg ccggggcaat cctcgccgcg actttgaatt acaagaggcc accattactg
    11581 attcgcgtta cctggatggc ttaagcatta ttgttgaagc gccggaagcc ggttcgttag
    11641 gtattggtac gcctgtgctg ttccgtggtc tggaagtcgg tacggttaca ggaatgacgc
    11701 tggggacatt gtcagatcgc gtgatgattg cgatgcgcat cagtaaacgc tatcaacacc
    11761 tggtgcgtaa caattccgtc ttctggttgg catcgggtta cagtctggac tttggtctga
    11821 cgggcggcgt agtgaaaacc ggcaccttta accagtttat ccgtggcggc atcgccttcg
    11881 ccacgcctcc gggtacgcca ctggcaccga aagcccagga aggcaaacac ttcctgttgc
    11941 aggaaagtga accgaaagag tggcgtgaat ggggaactgc gcttcccaaa taatgcccac
    12001 tgctccggcg tgcctgcgcc ggagcgttta tgctaaactg cgcgcctgtt tttttgccag
    12061 tggtacatgc tcgtggccca acacaccgtt tatttcccgg acgcctttct gacacaaatg
    12121 cgcgaagcga tgccttcgac gctctcattt gatgattttc ttgccgcctg tcagcgcccg
    12181 ttgcgccgca gcattcgcgt taatacgctg aaaatctccg ttgctgattt cctgcaatta
    12241 accgctcctt atggctggac gcttacgcca attccgtggt gtgaagaagg tttctggatt
    12301 gaacgcgaca atgaagatgc attgccattg ggtagtaccg ccgagcattt aagcggcctg
    12361 ttttatattc aggaagccag ttcaatgttg cccgttgccg ccttgtttgc tgacgataat
    12421 gcaccacagc gggtgatgga tgtcgcagct gcgccaggct ccaaaacgac gcaaattgcc
    12481 gcgcggatga ataacgaagg ggcaatcctt gccaatgagt tttccgccag tcgggtaaaa
    12541 gtgttacatg ccaatatcag ccgctgtggc atcagtaatg ttgcgctcac acattttgat
    12601 ggccgcgtgt ttggtgcggc agtgccagaa atgttcgatg ccattttgct ggacgctccc
    12661 tgctctggcg aaggcgtggt gcgtaaagat cccgatgcgc taaaaaactg gtcaccagaa
    12721 agcaatcagg aaatcgcagc tacacaacgg gagcttatcg acagcgcctt tcatgcatta
    12781 cgtcctggtg gtacgctggt ttactcgacc tgtaccttaa accaggaaga aaacgaagcc
    12841 gtttgcctgt ggctgaaaga gacttacccc gacgcagtag agtttttacc acttggcgat
    12901 ctcttccctg gtgcaaacaa agcgctgacc gaagaaggct ttttgcatgt tttcccacaa
    12961 atttacgact gcgaaggctt cttcgttgct cgtctgcgta aaactcaggc gattcccgcc
    13021 ttacccgccc ccaaatacaa agtcggtaat tttccgttca gcccggtgaa agatcgcgaa
    13081 gctggacaaa ttcgtcaggc ggctacaggt gttggcttaa actgggatga aaacctgcgc
    13141 ctctggcagc gtgacaaaga actgtggttg ttcccggtgg gcattgaagc cctgatcggt
    13201 aaagtccgat tttctcggtt ggggattaaa cttgccgaga cgcacaacaa aggttatcgc
    13261 tggcagcatg aagcggttat tgctcttgcc tcacccgata atatgaatgc attcgagctg
    13321 acaccgcagg aagcggagga gtggtatcgc gggcgcgatg tttacccgca agccgcgcca
    13381 gtggcggatg atgtattggt tactttccag catcaaccga ttggtttagc caaacggatt
    13441 ggttcgcgat tgaaaaacag ctatccgcgt gaactggtgc gcgatgggaa actttttacc
    13501 ggtaacgcct gacagcgcac aaaaaaagcg cactttttga ctggcacatt cggctgcctc
    13561 aactaggctg aaaaatggtg cgatcggact ggtcgtacca caatcggcag ctaaatggag
    13621 agcacgaaga tgaaaaccag tgtgcgcata ggcgcttttg aaatcgacga cggcgaatta
    13681 cacggtgaat cgccaggtga tcgaacgtta accattcctt gtaaatctga ccccgattta
    13741 tgtatgcaac tggatgcctg ggatgctgaa accagtatcc ctgccctgct aaatggcgaa
    13801 cactctgtcc tttaccgtac ccgttacgat caacaatctg atgcctggat tatgcgtctt
    13861 gcctgatcca aaaagaaccc gtcggcatgg cgggttattt gtcctggtta ttcccccgtt
    13921 gtaaaatctc tcctaaactt aacggtacgg caccacactt cggggatgaa atgttcgcgc
    13981 tggtactttt tgtttgctac ctggatggcg gttgtgaaga tattgttgtg gatgtctaca
    14041 acacggaaca gcagtgtctt tattctatga gcgatcaacg gatccgccat ggcggttgtt
    14101 ttccgattga ggattttata gatggtttct ggcgaccagc acaggagtat ggtgattttt
    14161 aattattgca attgcacaag agtcagttcg cccccaaaga cagcaccggt atcaatataa
    14221 tgcaggttgc caatatccac gcgatgtcgc aacggtgtat gaccaaacca gaaatgatca
    14281 gcacctgtaa ttccctgccc tttttggcgt tcacctaatc gcgagcggct ccacaagacc
    14341 tgatgcaaat caacgtcctt ttgccattca taaacatcat ctggataatc ggcatgagca
    14401 ataacatgtt tgccggtacg actgtgtact tcaagaataa agggcaaatg ctgacatttt
    14461 tccagcgccg ttttcgcttg tttctgttga ttatctgcca gcgcaataaa ccagtcgccg
    14521 ccattcatca accacaaaga catctgctgg gatgccagcg catccatcgc catctgttca
    14581 tgattgcctc ttaccgcaca aacccaatgt tgttccagta actgcagaca acgtaaactt
    14641 tgcggcccac gatcgataac gtctcctact gagataagta aatctcgcca cggatcaaaa
    14701 cgacaatgcc ataatttgcg gcgcaactgc tcaagacaac cgtgtatatc gccagaaagc
    14761 cagatatgtc gccattgatg acccgcaatt ctctgataaa cgggcgcagg ctgtttcatc
    14821 aatattttcc tcccgcgcta aagatcacat aatcttaaca agaatgttaa aaaacgctgg
    14881 actcagacag tagagtgtgt gttatggttg actataaagt cagcgaagga aatgcttctg
    14941 gcttttaaca gataaaaaga gaccgaacac gattcctgta ttcggtccag ggaaatggct
    15001 cttgggagag agccgtgcgc taaaagttgg cattaatgca ggcttagttg ccttgccctt
    15061 taagaataga tgacgacgcc aggttttcca gtttgcgtgc aaaatggtca ataaaaagcg
    15121 tggtggtcat cagctgaaat gttaaaaacc gcccgttctg gtgaaagaac tgaggcggtt
    15181 tttttattgg aaatcaaaag gctattttag gtaattaaca gagtttttca gctcgttcta
    15241 taaacggtgc cagactcatt ttttcgccgg gattgttagg atcatcaatc tgaatcaccg
    15301 aaatgggttg ggctttagtc ttcccactgg caacttcctt ttgtgcgata tcgtttaaag
    15361 gatactgcac gagggtactt gggttaatga catacaaagc attacccggt ctgcaagtca
    15421 gcatcacctc ttcgcgatta aacgcccatt tgtctttacc cacttcaaaa cgactgacgg
    15481 taatcacctg cggtgcagcc agcgccgctg cagaactggt gagtaacaga aacggcagaa
    15541 tacttttttt catcattttc gtaatccatc aaaaaggttc gagagtcgca agcaggctga
    15601 tgatcaccag cactaccgca tctatcgccc attcgagttt agtcatccac tcaaaccagg
    15661 gtgccgcacg atcttcatct tgcctcatgc gcggtacaag aacataacga ttagccagcg
    15721 caatgaccac cataatcatc accaggatgg ctttgagcaa taagagttgg ccccagtaag
    15781 tcgtaagcgt ggggggaaat ccggtaatca acaatgcatt aagcacgccg cttgccagta
    15841 cgccgatcac cgcaaaatgc ccgcaccagg aaaaacgcat cagcgcctga atggcctggt
    15901 gtcgccaacg gcctttgatg agttgcatac accagagcac cggcagtaat cccccaaacc
    15961 acgccgccgc acaaatcagg tgaatcgcgt gattagtctg atggattttc gccgttaccc
    16021 cttcattcag tgtcgcatgc ccgattcccg ccagcagaat aaattgcgcg gtagtgagca
    16081 taaacagcaa acgtggcata ttccgcggtt gcataagggc gacgatcaac gttacgaggg
    16141 cgagaacaat ttgccataac cagacgccac caaactgcgt ttgcaaaaca gcctgccaaa
    16201 tgtttggcga aaatacatct gcccatcccg ttcccatcaa cccgccctga acggcgagca
    16261 ttgcagtggc actgatcaaa ctccagacgg cagcgtgctg ttgtaaacgc agaaaacgct
    16321 tcgccagcaa gcgacgaatc gttaaggggg ccagccaggc accgtacatt gcgaagccaa
    16381 agaccagcat cagcgaggtg aaatggataa atcgcagcgc gatccaggta aacgccagca
    16441 tgatttattt cacgctaaag gtgtagtgtc ctttcgtttt atgcccgtcc accgaaacaa
    16501 catgccagtc aacgatgtag gttccgggtt tcagcgaatc ggccagtgga acaatcagtt
    16561 gcttttggtc ctgctcattt cgcttcgccg gtaatgtttt aatattttcg ttttttggcc
    16621 ccgtgatttt tgcaccactg aatcctgttt caacaccttc cgagaagttt aaagtgattg
    16681 cctgcggtgc agctgtcact tgcgcgtttg ccgcaggata ctgatgcgtt aaatgcgcat
    16741 gtgcccagac ggaaggtgtc accaacgaag tggtcaggat tgccagcgcg tagcgaaggg
    16801 agcgtgcagt tgaagtcata ttatctattc ctttttgtaa taactttttt acagagcata
    16861 accttgtcta atgtctgagt cgaggatcat caattccggc ttgccatcct ggctcactct
    16921 tagtaacttt tgcccgcgaa tgatgaggag attaagaatg ctgaagaatc tggctaaact
    16981 ggatcaaaca gaaatggata aagtgaatgt cgatttggcg gcggccgggg tggcatttaa
    17041 agaacgctac aatatgccgg tgatcgctga agcggttgaa cgtgaacagc ctgaacattt
    17101 gcgcagctgg tttcgcgagc ggcttattgc ccaccgtttg gcttcggtca atctgtcacg
    17161 tttaccttac gagcccaaac ttaaataaaa cttatacaga gttacacttt cttacataac
    17221 gcctgctaaa ttatgagtat tttctaaacc gcacccataa tttgcagtca ttttgaaaag
    17281 gaagtcatta tgtcgttctg gaaagttgca gcagcgcaat atgagcctcg taaaacctca
    17341 ctcaccgaac aggtagctca tcatctggag tttgtgaggg ccgccgccag gcaacaatgt
    17401 cagctgttag tttttccatc gctttcttta ctggggtgtg attattcgcg acgtgccctt
    17461 cctgccccac ccgatctctc actgttagac ccgctttgtt atgccgcaac gacctggcga
    17521 atgaccatca ttgccggcct tcccgttgaa tataacgatc gctttattcg tggaattgcg
    17581 gtgttcgcac cgtggcgaaa aacgcctggg atctaccatc agagtcatgg tgcatgtctg
    17641 ggtcgtcgtt ccagaaccat cacggtggtc gatgaacaac cgcaaggtat ggatatggac
    17701 ccgacctgtt cactgtttac tacagggcaa tgtctgggag aacccgacct gctggcgtcg
    17761 gcccgtcgct tacaattttt ttcacatcag tactcgattg ccgtgctgat ggcaaatgcc
    17821 cgtggtaaca gtgcactatg ggatgaatat ggtcgtctca tcgttcgcgc cgatcgtggt
    17881 tcattattgt tagtcggtca gcgttcatca cagggttggc aaggcgatat cattccatta
    17941 cgctaggctt tttcggcctg gagcatgcca tgttgcgcat tatcgataca gaaacctgcg
    18001 gtttgcaggg agggatcgtt gagattgcct ctgttgatgt cattgacgga aaaatcgtca
    18061 accccatgag ccacctggtg cgccccgatc gtcctattag tccacaagcg atggcgattc
    18121 atcgcatcac cgaagccatg gtcgccgata aaccgtggat tgaagatgtg atcccacact
    18181 attacggtag tgaatggtat gtcgcgcata acgccagctt tgaccgccgc gtactgcctg
    18241 agatgcccgg tgagtggatt tgcactatga aactggcccg tcgtttgtgg cctgggatca
    18301 agtacagcaa tatggcgtta tataaaacac gcaagctcaa tgtacagacg ccgccgggcc
    18361 tgcatcatca ccgcgcgttg tatgactgtt atatcaccgc cgcgttgctt atcgatatta
    18421 tgaacacctc cggctggacg gcagaacaga tggccgatat caccggacgt ccgtcgttga
    18481 tgacgacctt cacctttggc aaataccgtg gcaaagcggt ttccgacgtt gccgaacgcg
    18541 atccgggcta tctgcgctgg ttatttaata acctggacag catgagcccg gagctgcgtt
    18601 taacactgaa acattatctg gaaaatactt agtccgcagg cgtagcgggt aatgttccct
    18661 gcgccagcgc gaccagaaaa gcatattcca tcgctacgcc ttcgtacgat ttaaagcgac
    18721 cagatttacc gccatggcct gagtccatgt cggtacagag caataaaaga tggtcatcgg
    18781 ttttcagctc gcgcaattta gcgacccatt ttgccggttc ccaatattgc acctgagaat
    18841 cgtgcaaacc ggtcgttacc agtaaatgcg gataagcctg tgcggtgacg ttgtcatatg
    18901 ggctgtagct tttcatgtac tcgtaatatt gcggatcctg cggattcccc cactcttcaa
    18961 actcaccagt ggtaagagga attgattcat caagcatcgt tgttacaaca tcaacaaacg
    19021 gtacctgggc gataacgccg tggaataatt ccgggcgttg attaattgca acgcccatca
    19081 acatgccccc cgcactcccg cccatcgcat aacaaagcga aggagagcca tagcccagtt
    19141 ttaacaatgc atcgcaggca tcaagataat cattaaacgt atttttcttc ttcagaaatt
    19201 ttccgtcttc gtaccattgt tgccccagct caccaccgcc gcgaacatgg acaatggcgt
    19261 agacaaagcc acgatctaac aaactcaagc ggctaaaact gaaatcggca tcaatacttg
    19321 cgccgtaaga accatagcca tacaccagca acgggttgtg tcctttgcga aaatgtttgc
    19381 gatggtagac caacgaaacc ggaacttcga cgccatcacg ggcgactatc cacaggtgtt
    19441 cactgcggta attcgccgca tcaaaaccgg gcacttctgt ttgttttaat acacgacgct
    19501 caccggtatc catatccagt tcaaacaaag tgtctggtgt ggtcatggaa gaataaccat
    19561 aacgcaatcg cgcggtttca ggttctggat tgtaggcaat ccaggtcaca taggctggat
    19621 catcaaaggc aataccaatg acttcccggg tcttgcggtt aatttggcgc aaactggtta
    19681 acccgcgctg acgctcttca accaccagcc agtcggtaaa cagcgtaaac ccttccagca
    19741 tgatgttttc gcgtggcgga attaactctt cccactgttg ctcatcacgc atacgggtac
    19801 ggtataagcc aaagtttttg ccgtggcggt tggaacgcag ataaaaacga tgctggtagt
    19861 gatcaaggct gtattcgtga tctttgcggc gcggcagaaa aacaaacggc tcggcatcgg
    19921 ccatttccgc gtccagcagg cgaacttcac tggtggtggc gctggccaaa tgaatgacta
    19981 catagtgctt cgaggtcgtt ttatgcaggc tgacgtaata ggtatcgtct ttttcttcgt
    20041 agatcagttt atcttgcgat gctggcgtac cgatggcgtg acgccagacc tgataaggca
    20101 gcagcgtcac cggatgcttg cgaacatagt agaaagtcca ggagtcattt gcccagacaa
    20161 agctgggttc aacgttatcc agcagttccg ggtaccagtt accagtttcc agattacgaa
    20221 aacgaatgcc gtactggcgt cgggaaagaa aatcttctgc cagcgccata atggtgttat
    20281 cgggcgtaat cgccattccg cccatcgaat aaaactcact atgagctgcg cgcttattgg
    20341 catcgagcaa tgtttcccac tcatcccact cttcactgaa tgccgattga cgctggtaga
    20401 tagcatattc acagcctggt tcataaatat gccgatagcg gtagccattt ttgatgtagg
    20461 gcgcagaaac ttctcgttgc ggaatgcggt cgatgatttc ctttaagatg cgatcctgca
    20521 aggcttgttg tgaggccatc acccgatgac cgtaactatt ttcttgttgc aggtagtcca
    20581 ggacttctgg ctgagaacgc gtatcgtccc gcagccagta gtaattatcg atgcgcgtat
    20641 cgccatgaag cgtcatggcg tggggaatgc gggcggcttt tggtagcatg ttattgttct
    20701 ttctggttga aacatcttat aagggtggca aaactcaccg gggatgcaag cgaaacaggg
    20761 gagtcattgc ttagatgatg acaggtaatg gcgcggatat cgaatgttat gcagacagag
    20821 aaaatcagcc tgttcaaatg gctgtgcgat tctggatagc ccgaaatagt caacttcagg
    20881 ctatccagag agcggaatta ttccgccaaa gtgcgttttt gctgttcgag atcgcgttca
    20941 atgccgtcac gaacatcctg ggggattttc agcgcgtcac ccagtgcatt caggtaactg
    21001 cgttccataa aatggtcaat atcaatagcc gcgcaactca ggaaatagat ttccagcgcc
    21061 tcttcttcat tgcggacccc ggtagccagg cgttgtggat ccagcggttg ttcgattgcc
    21121 tgctcaatga gtacacgccc ctgctcttcc acgccggctt cacgcaattg ctggtcgatt
    21181 gccgcacgtt ctttggcatc aatatgacca tcacttttag cggcaaagac cagcgcaagg
    21241 atcaaacgtt ctgtacgctc atccagcggc gtactttgcg cgccaaactg cggttcgtcc
    21301 tgatgcgccg cgcgaatttt atctttgtat ttattccaca gcaccgtacc cgctaccgct
    21361 ccgccgccaa ccagtaacgc gttggtgcca tatttcgtaa gaagtttacg tgctgattta
    21421 tttgcgacca gcagcccagc cagtccgcct aatgcgcctg gcactaacag tttgcccaat
    21481 ccctgatccg cagacgagga ggtagaagaa ctgctttgcc caagcaggga ttgcagttga
    21541 tttaaccagt tagccatatt tgctcctcaa taaccatttt tagtgcctgt agcatagcgg
    21601 atgaggatgt cagaaaatgt atatcgagac aaaagatgcg caaataagcg gggcggtagg
    21661 ccgttattca aagaaattcg cgccaggtaa ggtatttact gtatatcagg tgataagggt
    21721 attttaggtg aaagtagttt gtcgttttga gtggcattgt ttgatgctga ttgcccgatg
    21781 cgacgctgtt gcgtcttctc aggcctacga ttgtggtgcg ttctggggcg gacaaggcac
    21841 tcgcgccgca tccgccaacg gtcaatcgaa ttatcagaag ccattaacgc cgctgatatt
    21901 ccgccattcc cgctttgcag tccacgctga cctggtaatg aatatcggca cttttaccac
    21961 gcacggttag cggtactgac catttatcat ctttaccctg aatgtcctgc aaactgaccc
    22021 aagccacggg atcggcctga ccgacaattt tttgatcatc tgcccagcgc gccacgcgat
    22081 tttgttgata atcacgtttt acgctcgcgg caattccggc agcatccaga ccttcacact
    22141 ttgggaaagt gaccgacttg ctggtttcat tattggcagc gaaaactgat gcgcaggcag
    22201 aaaccaacaa cagccctaaa aacgcccctc tttttttcat gtttttctcc atagcacaat
    22261 gattcaggag aaagcatggt acaaattgtc aggagcgcaa gttgcttcag gccgcgtgtg
    22321 aggcatcttc ctcttcggat tcagcaccga cgacctgtgg ggacggtaat ttgccggttt
    22381 tcagaatggt gctcagcaca tctttttgtt ctgccagcca tagcgaaagg gcttcacgtt
    22441 gctcgtcttc catttgcact ggagaatttg tcagccaggt gtcaagcaga tccgccgtat
    22501 caagcatttt atcataagca tcggcttcct ttttgctggt aaacgacatt ttctcttcgc
    22561 cctcacgaat gactacgtat ttaacttcaa ccgccatttg cagcctctca taataactgt
    22621 gattttatac agtatatttc ttttcggttg agaaatcaac atcagcaata aagacacacg
    22681 caaacgtttt cgtttatact gcgcgcggaa ttaatcaggg gatattcgtt atgacgttat
    22741 taggcactgc gctgcgtccg gcagcaactc gcgtgatgtt attaggctcc ggtgaactgg
    22801 gtaaagaagt ggcaatcgag tgtcagcgtc tcggcgtaga ggtgattgcc gtcgatcgct
    22861 atgccgacgc accagccatg catgtcgcgc atcgctccca tgtcattaat atgcttgatg
    22921 gtgatgcatt acgccgtgtg gttgaactgg aaaaaccaca ttatatcgtg ccggagatcg
    22981 aagctattgc caccgatatg ctgatccaac ttgaagagga aggactgaat gttgtcccct
    23041 gcgctcgcgc aacgaaatta acgatgaatc gcgagggtat ccgtcgcctg gcggcagaag
    23101 agctgcagct gcccacttcc acttatcgtt ttgccgatag cgaaagcctt ttccgcgagg
    23161 cggttgccgc cattggctat ccctgcattg taaaaccggt gatgagctct tccggcaagg
    23221 ggcagacgtt tattcgttct gcagagcaac ttgctcaggc atgggaatac gctcagcaag
    23281 gcggtcgcgc cggagcgggc cgcgtaattg ttgaaggcgt cgttaagttt gacttcgaaa
    23341 ttaccctgct aaccgtcagc gcggtggatg gcgtccattt ctgtgcacca gtaggtcatc
    23401 gccaggaaga tggcgactac cgtgaatcct ggcaaccaca gcaaatgagc ccgcttgccc
    23461 ttgaacgtgc gcaggagatt gcccgtaaag tggtgctggc actgggcggt tatggtctgt
    23521 ttggtgtcga gctgtttgtc tgtggtgatg aggtgatttt cagtgaggtc tcccctcgtc
    23581 cacatgatac cgggatggtg acgttaattt ctcaagatct ctcagagttt gccctgcatg
    23641 tacgtgcctt cctcggactt cctgttggcg ggatccgtca gtatggtcct gcagcttctg
    23701 ccgttattct gccacaactg accagtcaga atgtcacgtt tgataatgtg cagaatgccg
    23761 taggcgcaga tttgcagatt cgtttatttg gtaagccgga aattgatggc agccgtcgtc
    23821 tgggtgttgc gctggcgacc gctgagagtg ttgttgacgc cattaaacgc gcgaagcacg
    23881 ccgccggaca ggtaaaagta cagggttaaa cccggcaaaa aaacgctaca aaaatgcccg
    23941 atccgcgatc gggcattttg acttttacag cttagcgcct tctacagctt cacgcgccag
    24001 cttagtaata cggtcgtaat cgcccgcttc cagcgcatct gccggaacca gccaggaacc
    24061 accgatgcac agcacgcttt tcagcgccag gtagtcacgg tagttagccg gagaaatacc
    24121 acctgtcggg cagaaacgga cctgggagaa cggacccgcg atcgcctgca gggctttcac
    24181 gccgccgtta gcttcagctg ggaagaattt gaactctttc aaaccgtagt ccatacccag
    24241 catcagttcg gaaacagtgc tgatccccgg aatcagagga atagtccctt cggtagcagc
    24301 tttcagcagc ggctcggtca gacccgggct aattgcgaac tgtgcacccg cttcggtgac
    24361 ttctgccagc tgctgtggat tcagcaccgt accggcaccc acaatcgctt caggcacttc
    24421 tttggcgata gcacggatag cgtcaactgc acactcggta cgcagagtca cttccagaac
    24481 gcgcacccca ccagcaacca acgcttttgc catcggcact gcgtgttcca gtttttttac
    24541 cacgataacc ggtacaaccg ggccggtggt caggattgat tctgcacttg ttttccagtt
    24601 tttcatcaga gttttctctc gcctgattac aaatttgtcg tcttaaaaag tgatacaggt
    24661 tgcgccctgt tcggcaccgg acagtttttc acgcaaggcg ctgaataatt cacgtcctgt
    24721 ccccacgcgt gacgcgctca ggtcaggaat gtgcggttcg cgagcagcca gttccgcttc
    24781 gtctaccagc agcgtcagtt cgcctgtctg tccattcaca cgaatgatgt ccccgtcgcg
    24841 cacttttgcc agcagcccgc catcgtaggc ttctggtgtt acgtggatag ctgacggcac
    24901 tttacctgaa gcgccggaga gtcgtccatc ggtaactaac gcaattttga aacaccggtc
    24961 caataataca ccaagtggcg gcatgagttt atgtaattct ggcattccgt tcgcttttgg
    25021 cccctgatga cggacaacaa cgacacaatc gcggtccagc aaacccgctt caaaggccgg
    25081 cataacgtca tgctggcttt caaaaacaac cgctggcgct tcaatcacct ggttctcaac
    25141 cggcacggca gaggttttca taaccgcacg gcccaggtta ccgcttaaca cttttgtccc
    25201 accatgatga gagaaaggtt gttcgaagga agcgatcaca ttgctgtcga gtgatttttc
    25261 cgccccttcc cgccagtcca gttcaccatt attcagccat ggttcaaggg tataacgaga
    25321 cagaccaaag cctgccaccg tattgacatc ttcatgcagc aggcctgctt tgagcagttc
    25381 acgcaccaga accggtacgc cacctgccgc ctggaagtgg ttaatatcgg ccggaccgtt
    25441 cgggtagaga cgtgccatca gcggtacaac atcagaaagg tcagagaagt catcccagtt
    25501 aatctgaata ccggccgcgc gcgccatcgc caccaggtgc atggtgtggt tagtggaacc
    25561 accggtcgcc agcagtgcaa cgataccgtt caccaccact ttctcatcga tcatcttacc
    25621 gatcggcatc cattcattac cattaccggt catgcgtgta acctgacgcg cagcggcagc
    25681 ggtcaaagca tcgcgcagcg gagaatccgg atgaacaaaa gaagagcctg gcaactgcat
    25741 ccccataaat tccaccacca tctggttggt gttggcagta ccgtagaaag tacatgttcc
    25801 cggcgcatgg taagacgcgg cttctgactc cagtaaggcc atgcggtcca ctttaccttc
    25861 ggcataaagc tggcgaatac gcactttttc tttatttggc aaaccgcttg ccatcggtcc
    25921 agacggcaca aacaccgcag gcaaatgacc aaacgacagg gctgccatcg tcagacccgg
    25981 gacaatcttg tcgcacacac cgaggaacag agcaccatca aacatgttat gggacagccc
    26041 gaccgccgca gacatcgcta tcacttcgcg gcttagcagc gacaattcca ttccatcctg
    26101 cccctgggtg acaccatcac acatcgccgg aacaccgccc gcaacctgac caaccgcatt
    26161 cgcttcatgc agggctttac gaatgatttc tggatagtgt tcataaggct ggtgcgcgga
    26221 gagcatgtcg ttataggagg tgatgatggc gatattgtta cgcaacatgc ttttcaaaga
    26281 ggctttgtct tctggctggc aggcagcgaa accgtgtgcc aggttaccgc atgccaactg
    26341 cgaacgatga acggtcgaag ttttcgcttg ttctatccgg gcgagataag cagagcgagt
    26401 ctcgcgcgaa cgttcaatga ttcgatttgt tacgcgtaac aattgtggat tcataaaggc
    26461 tcctgaaatt gagttgtcag agcaggatga ttcacaacgc gtttcattca gaggatttat
    26521 gactgaaacg cctgtaaccg gagctcatag ggcaaaaacg tttcagtcag tgtaataaaa
    26581 aaagcctcgt gggtgaatcc gcacgaggcc tgaaagtgta aaaattgttc tacaatctgt
    26641 gcaagatcat gttaccggta aaataaccat aaaggataag cgcagatatt actcaaactc
    26701 attccaggaa cgaccatcac gggtaatcat cgccaccgag gcaacgggtc cccaggttcc
    26761 ggcctgatac ggtttcggcg catcattgtc catcgcccac gcctcagtaa tggagtctac
    26821 ccatttccag gcttcttcca cttcgtcgcg acgaacaaac agtgcctgaa taccacgcat
    26881 ggtttccagc agcaaacgtt cataggcatc cgccagatgc gtctgattaa aggtttctga
    26941 atagctcaga tccagcttgg tgatttgcag gttatgtttg tggtcaaggc caggaacttt
    27001 attcagtacc tggatatcca cgccttcatc aggttgcaga cggatagtca gtttattctg
    27061 cggcagatcc tgccacgatt ctttaaacag attcagttca ggtgttttga aatagaccac
    27121 gacttcagaa catttggtcg gcagacgttt accagtacgc aggtagaatg gcacaccggc
    27181 ccagcgccag ttatcaatgt cgacgcggat cgctacgaag gtttctgtat tgctgctctt
    27241 gttcgcgccc tcttcttcca gatatcccgg cacttttttg ccctgggcga agcccgcagt
    27301 atattgcccg cgtacggttt tttcgcgtac gttggagcgg tcgatgcggc gcagagactt
    27361 cagtactttc actttttcat cgcggatgct gtctgcgctc aggtcagacg gcggagacat
    27421 cgcaatcatg cagagaattt gcagcaggtg gttttggatc atatcgcgca tctgaccggc
    27481 tttatcaaaa tagccccagc gcccttcgat ccccacttct tctgccacgg taatctcaac
    27541 atgatcaatg gtgcgattgt cccagttatt cacaaacagg gagttagcaa aacgcagcgc
    27601 caacaggttc agcaccgttt ctttaccaag atagtggtcg atacggtaaa cctggcactc
    27661 ctcgaagtat tcgccaacct gatcattgat ttcctgcgag gtcgccagcg acgtccccag
    27721 cggtttctcc atgactacgc gtgccggttt agcattcagt ttagcctcgc caagcccttt
    27781 gcaaattgcg ccaaaagtgc tgggcggcat ggcaaagtag ttaatggtga tacgattttt
    27841 ttgatccagc atcgcgccga gacggctgaa tgcagcagtg tcattgacat cgagattaca
    27901 aaaatccaga cgtgcactca gggtgtccca taaaccttca tcaatggttt ctttcatgaa
    27961 agtttcgagc gcctcgcgga caactttggt atatgccgct ttatcccagt cagcacgccc
    28021 tacgccgata atccgggtgt ccgggttgag ctgaccggct ttttccagtt gatacaggga
    28081 aggcagcaat ttacgacgcg caaggtcgcc tttcgcgccg aaaatgacca ggtcacaggc
    28141 ctgggctgtt tgcgttaccg ccatgtcatt ctccttaagt taactaaccc ggtacttaag
    28201 ccagggtata cttgtaattt tcttacggtg cactgtactg cttttacgag cttgcgaaaa
    28261 ctgtaaacgc ttatccaccc gtgcgattac ggtaaaagcg cgcaaagtgc ggcaaaactg
    28321 ataaaaaaat catcgttttc cattgggtaa aaatctgaca ctgatcatgt tatgaaaaaa
    28381 aataacaact tttttatctg cttttgtcat taacacagca cacaggcgta atatctgaca
    28441 aaactacatt tcgatttctt tcagtgcgga aatcgtcatt acccgtgagt ttctttacat
    28501 catgaatatg ctggaaaaaa tccagtctca gctggaacat ttgagcaaat cagagcgcaa
    28561 agttgccgag gtcattctgg cttcgcccga taacgcgatc cattcgagta ttgctgctct
    28621 ggcgctggaa gccaatgtta gcgaaccgac ggtgaatcgt ttctgtcgca gcatggacac
    28681 gcgcggtttt cctgatttta aacttcatct ggcacaaagt ctggcgaatg gcactcccta
    28741 tgttaatcgc aatgtcaatg aagatgacag cgttgaatca tacacaggga aaatatttga
    28801 gtccgcaatg gcaacgcttg atcatgtccg tcattcactg gataaatctg ccatcaaccg
    28861 cgccgtcgac ttgctcactc aggcaaaaaa aatcgccttt ttcggattag gctcttcagc
    28921 cgccgttgcc cacgatgcga tgaataagtt ctttcgtttt aatgttccgg tggtgtactc
    28981 cgatgatatc gtgctgcaac gcatgagttg tatgaattgt agcgacggag acgtggtggt
    29041 gctgatttct cacactggaa gaacaaaaaa tctggtcgag ctggcgcagc tggctcgcga
    29101 aaacgacgca atggtgattg ccctcacctc tgcgggtacc ccgctcgccc gggaagcaac
    29161 gctggcaatt accctcgacg taccggaaga tactgacatt tatatgccca tggtttctcg
    29221 acttgcacag ctgaccgtga tagatgtgct ggcgacagga tttactttgc gacgcggtgc
    29281 aaaattcaga gataacttga agcgggtcaa ggaagcgctg aaggaatcgc gttttgataa
    29341 gcagttactt aatttaagtg acgatcgcta aaaacgactg tcactgtcct aatcttatac
    29401 gacatccgaa tgagattaat ttatcgccat cgcggcgtta tttcattcgg atttcatgtt
    29461 caagcaacac ctggttgttt cagtcaacgg agtattacat gtccagaagg cttcgcagaa
    29521 caaaaatcgt taccacgtta ggcccagcaa cagatcgcga taataatctt gaaaaagtta
    29581 tcgcggcggg tgccaacgtt gtacgtatga acttttctca cggctcgcct gaagatcaca
    29641 aaatgcgcgc ggataaagtt cgtgagattg ccgcaagact ggggcgtcat gtggctattc
    29701 tgggtgacct ccaggggccc aaaatccgtg tatccacctt taaagaaggc aaagttttcc
    29761 tcaatattgg ggataaattc ctgctcgacg ccaacctggg taaaggtgaa ggcgacaaag
    29821 aaaaagtcgg tatcgactac aaaggcctgc ctgctgacgt tgtgcctggc gacatcctgc
    29881 tgctggacga tggtcgcgtt cagttaaaag tactggaagt tcagggcatg aaagtgttca
    29941 ccgaagtgac cgtcggtggt cccctctcca acaataaagg tatcaacaaa cttggcggcg
    30001 gtttgtcggc tgaagcgctg accgaaaaag acaaagcaga cattaagact gcggcgttga
    30061 ttggcgtaga ttacctggct gtctccttcc cacgctgcgg cgaagatctg aactatgccc
    30121 gtcgcctggc acgcgatgca ggatgtgatg cgaaaattgt tgccaaggtt gaacgtgcgg
    30181 aagccgtttg cagccaggat gcaatggatg acattattct cgcctctgac gtggtaatgg
    30241 ttgcacgcgg cgacctcggt gtggaaattg gcgacccgga actggtcggc attcagaaag
    30301 cgttgatccg tcgtgcgcgt cagctaaacc gagcggtaat cacggcgacc cagatgatgg
    30361 agtcaatgat tactaacccg atgccgacgc gtgcagaagt catggacgta gcaaacgccg
    30421 ttctggatgg tactgacgct gtgatgctgt ctgcagaaac tgccgcaggg cagtatccgt
    30481 cagaaactgt tgcagccatg gcgcgcgttt gcctgggtgc ggaaaaaatc ccgagcatca
    30541 acgtttctaa acaccgtctg gacgttcagt tcgacaatgt ggaagaagct attgccatgt
    30601 cagcaatgta cgcagctaac cacctgaaag gcgttacggc gattattacc atgaccgaat
    30661 cgggtcgtac cgcgctgatg acctcccgta tcagctctgg tctaccaatt ttcgccatgt
    30721 cgcgccatga acgtacgctg aacctgactg ctctctatcg tggcgttacg ccggtgcact
    30781 ttgatagcgc taatgacggc gtagcagctg ccagcgaagc ggttaatctg ctgcgcgata
    30841 aaggttactt gatgtctggt gacctggtga ttgtcaccca gggcgacgtg atgagtaccg
    30901 tgggttctac taataccacg cgtattttaa cggtagagta agtacgttgc cggatgcggc
    30961 gaaaacgcca catccggcct acagttcaat gatagttcaa cagatttcga atattctgaa
    31021 gcaaacttga acttatcatc aggcgaaggc ctctcctcgc gagaggcttt tttatttgat
    31081 gggataaaga tctttgcgct tatacggctg gatttcgccc ggtttgcgag ttttcagcaa
    31141 ttttagtatc caggtgtatt gttctggtcg cggaccaaca aaaatctcga cttcttcatt
    31201 catccgccgc gcaatcgtat gatcatccgc ctctaacaga tcatccatcg gtgggcgcac
    31261 ctgaatcgtc agacgatgcg tcttgccatc ataaatcgga aacagcggta caacgcgcgc
    31321 acggcacact ttcatcaaac gaccaatcgc gggcaacgtc gctttatagg tggcaaagaa
    31381 atccacaaat tcgctgtgct ctgggccatg atcctgatcg ggtaaataat atccccagta
    31441 cccctgacgt accgactgga tgaatggttt aataccgtca tttctcgcat gcagacgacc
    31501 gccaaagcga cgacgcaccg tgttccagac ataatcaaaa accgggttgc cctgattatg
    31561 gaacatcgct gccattttct gcccttgcga ggccatcagc atggcaggaa tatcgacggc
    31621 ccaaccgtgc ggcaccagaa agataacttt ctcgttatta cgcctcatct cttcgatgat
    31681 ctccagccct tgccagtcaa cgcgcggctg aattttctcc ggtccgcgta ttgccaactc
    31741 agccatcatt gccatcgctt gcggcgcggt ggcaaacatc tcatcaacaa tcgcttcgcg
    31801 ttcagcttca ctacgttctg gaaagcagag cgacagattg attaacgcac gacggcgtga
    31861 gctttttccc agtcgtccgg caaaacgtcc cagccgtgcc agaatgggat cacggaactt
    31921 tggcggcgtt aaagcgatac ccgccatcgc tgctacgccc agccatgctc cccagtagcg
    31981 cgggtggcga aaggatttat caaactcagg aatgtattcg ctattatttt ttttcgtttc
    32041 catgcttttc cagtttcgga taaggcaaaa atcaatctgg tgatagtgta gcggcgcaac
    32101 ttgccccgca ccaaataaaa aaagccggta ctgactgcgt accggctgcg aatggatgtt
    32161 aattaatcaa accgtagctg cggcacaatc tctttggcct gcgccaggaa ttcgcgacga
    32221 tcggagccgg tcagcccttc ggtacgcggc agttttgccg tcagcgggtt tacggcctgc
    32281 tggtttatcc atacttcata gtgcagatgc ggcccggttg aacgtccggt attaccggaa
    32341 agcgcgatac ggtcgccacg tttcaccttc tgtcccggtt tcaccagaat cttgcgcaag
    32401 tgcatataac gcgtggtgta gctgcgacca tgacgaatag ccacataata acctgctgcg
    32461 ccactgcgtt tggcaaccac cacttcaccg tcacccactg aaagcactgg cgtaccttgt
    32521 ggcatggcga aatcaacacc tctgtgtggt gcaacgcgac ctgtcaccgg attagtacga
    32581 cgcgggttaa agttagacga gatacggaac tgtttcgccg tcgggaatcg caagaatcct
    32641 ttcgccagac cagtaccgtt acgatcgtag aatttgccat cctcagcgcg gatagcgtaa
    32701 taatctttac cttctgaacg caaacgtacg cccagcagct ggctttgctc acgtttacca
    32761 tcaagcattt ctcgagacat taacaccgca aattcatcgc cttttttcag tttgcggaaa
    32821 tccatttgcc actgcatggc tttaatcact gcgctcactt cggcgctggt taaaccggcg
    32881 tttctggcgc tggcaacaaa gctccccccg acggtacctt tcagcagatt gttaacccac
    32941 tctccttgct gcatttcgct ggtcatttta aaaccgttag cagcagtacg gtcataggtt
    33001 cgggtttcac gacgagacac ttcccaggtg aggcgctgca gttcgccgtc cgcggttaat
    33061 gtccaggaga gttgttgacc gattttcaga ttacgcaatt ctttgtcggc agcagccagt
    33121 tgggtgatat cacccatatc aataccatac tgattgagaa tgctgcttag cgtatcgcca
    33181 gtggaaacaa catattcatg cacgcccgct tcaccggcga ttttgtcatc cagttcgtcc
    33241 tggggaatgg cttcatcttc ttgtgcagct tgatcaatcg gctcactggc ttcaggtaag
    33301 agcgaacgaa tttcgttctg ttccagctca atggttttga caattggcgt ggcatcacgg
    33361 tgataaacat aaggccgcca gacagcgacg gccagagtaa gaacggtgag cgaccccaac
    33421 ataacgcggt gtggtcgcgg taaattatta aacgccaggg cgacagagcg ggctatctgt
    33481 tgcacgtaat cacttcctca ttaatctcct ttcaggcagc tcgcatactg gttggctaat
    33541 tgactcagga attctgaata gcttgtttta cccagtttga tattcgtccc caggggatcc
    33601 aacgttccca tacgaacgga tgtccctcgt gcgacgctct caacgaccgc tggcctgaac
    33661 tgtggctcag caaaaacgca ggttgctttt tgctcaacca actgtgttct tatttcatgt
    33721 aaacgctgcg cgccaggttg aatctcaggg ttaacggtaa aatgaccaag cggtgtcagt
    33781 ccaaactgtt tttcgaaata gccgtaagca tcgtgaaaaa cgaaataacc tttccccttg
    33841 agcggcgcga gctcgttacc aacctgcgtt tcggttgagg ctaattgtgc ctcaaaatcc
    33901 ttcaggttgg cgtcaagttt ggctcgactt tgcggcataa gttccactaa ttttccatgg
    33961 attgcaaccg ctgtagcccg cgctatctct ggggaaagcc aaagatgcat gttgaaatcg
    34021 ccgtgatggt gatcttcgtc acttttttcc gcgtggtcgt gatcatcatc atcgccgtga
    34081 atacttttca tcagcagcgg tttcacatct tctagctgtg caatcgttac ctgtttcgct
    34141 tctggtaatt tacttaccgg tttttgcata aacgcttcca tctccgggcc aacccaaacg
    34201 actaagtccg cgttctgtaa gcgttttaca tccgatggac gcagtgaata atcatgttct
    34261 gaagcgccgt caggcagtaa aacctccgtt tctgttacac catcagcaat ggcagaagcg
    34321 atgaacccaa cgggtttaag cgaagcgaca acggcggcat ctgcggcctg tgttgcacct
    34381 ccccagagag cggcggataa tgctgcgaaa agaagcgttt ttttatgtaa cataatgcga
    34441 ccaatcatcg taatgaatat gagaagtgtg atattataac atttcatgac tactgcaaga
    34501 ctaaaattaa catgacaagt ctggtttccc tggaaaatgt ctcggtttct tttggccaac
    34561 gccgcgtcct ctctgatgtg tcgctggaac ttaaacctgg aaaaattttg actttacttg
    34621 ggccaaatgg cgcaggtaag tcgacactgg tacgggtagt gctcgggcta gtaacacccg
    34681 atgaaggggt tatcaagcgt aatggaaaac tgcgcatcgg ctatgtaccg cagaagctgt
    34741 atctcgacac cacgttgcca ctgaccgtaa accgtttttt acgcttacgc cctggtacac
    34801 ataaagaaga tattttgcct gcactgaaac gtgtccaggc cgggcacctg attaacgcac
    34861 cgatgcaaaa gctctctggt ggcgaaacgc agcgtgtact gttagcgcga gcattgttaa
    34921 atcgcccgca attattagtg ctggatgaac ccactcaagg cgtggatgta aatggccagg
    34981 tggcgttata tgaccttatt gaccagttgc gtcgcgaact ggattgtggc gttttaatgg
    35041 tctctcacga tctgcatctg gtaatggcaa aaaccgatga agtgctgtgt ctgaatcacc
    35101 acatttgttg ttccggcaca ccggaagttg tttccctgca tccggagttt atttcaatgt
    35161 ttggtcctcg tggtgctgaa caactgggta tctatcgcca tcatcataat catcgtcacg
    35221 atttacaggg acgaattgtt ttgcgtcggg gaaatgatcg ctcatgattg aattattatt
    35281 tcccggttgg ttagccggga tcatgctcgc ctgtgccgcg ggtccgctgg gttcgtttgt
    35341 agtctggcgt cgtatgtctt atttcggtga tacgctggct catgcctcat tacttggtgt
    35401 cgcgtttggt ttgttgctgg acgtgaatcc attctatgcg gtgattgccg ttacgctgct
    35461 gctggcgggc ggtctggtat ggctggagaa gcgtccacag ctggcgatcg acacgttatt
    35521 agggattatg gcgcacagtg ccctgtcgct gggcctggtg gtcgttagtc tgatgtctaa
    35581 tattcgtgtt gatttgatgg cttacctgtt cggtgatttg ctggcagtga cgccagaaga
    35641 tctcatctct attgcgattg gcgtggtcat cgtggtggct attttgttct ggcaatggcg
    35701 caatttgctg tcgatgacga ttagcccgga tctggcgttt gttgatggtg tgaaattaca
    35761 gcgcgtgaaa ttgttgttga tgctggtgac ggcattgacg attggtgtag cgatgaaatt
    35821 tgtcggtgcg ttgattatta cttcgttgct gattattcct gcagctactg cgcgtcgctt
    35881 tgcccgtacg ccggaacaga tggctggtgt cgctgttttg gtggggatgg tggcagtaac
    35941 tggcggttta accttttccg cgttttacga tacgccggcg ggtccgtcgg tggtgctatg
    36001 tgcggcactg ttatttattc tcagtatgat gaaaaagcag gccagctaat ctatcgctga
    36061 acacatttgt cggatgcggc gcgagcgcct tatccgacct acggttcgct atctctggta
    36121 ggcctggtaa gacgcgaata gcgtcgcatc aggcacactg ccaatgtcgg atgcggtgcg
    36181 agcgaccaat ccgacttacg gcatttctgg cggcgttatg ccaaagtgat tccacgcccg
    36241 cgtcgtcgcc atacgcccac gcggtgtacg ctgcaaaaag ccttgctgaa tcaaataagg
    36301 ttccagcaca tcctcaatgg tttcacgttc ttcgccaatg gctgccgcaa ggttatccag
    36361 acctaccggc ccaccaaaga acttatcgat taccgccagc aataacttgc ggtccatata
    36421 atcgaaacct tcagcatcga cattcaacat atccaacgcc tgagcagcga tatctgccga
    36481 gatggtgcca tcgtgcttca cttcggcgaa atcacgcact cgacgcagca gacggttggc
    36541 aatgcgcggc gtaccgcgag ctcgacgagc aacttccagc gcgccgtcat cactcatctc
    36601 aagccccata aagcgtgcgc tgcgactgac gatatattgc agatccggca cctgataaaa
    36661 ctccagacgc tgcacaatac caaaacgatc gcgcaacggt gatgtcagcg aacctgcgcg
    36721 cgtggttgca ccaatcaggg taaacggcgg caaatcaatt ttaatggagc gtgccgccgg
    36781 accttcacca atcatgatat ccagttggta gtcttccatt gccggataca acacctcttc
    36841 caccactggc gaaagacggt ggatctcatc aataaacaac acgtcatgcg gttcaaggtt
    36901 agtgagcatc gctgccagat cgcccgcctt ttccagcacc ggaccagaag tcgtgcgtaa
    36961 attaacgccc atttcattgg cgacgatgtt ggcaagcgta gttttaccca accccggagg
    37021 accgaaaatc aacagatgat cgagggcatc gccgcgcagt ttcgctgctt tgatgaaaat
    37081 ctccatctgt gaacgaacct gcggctgacc aacatactct tccagtaatt tagggcgaat
    37141 ggcgcgatct gccacatctt ccggcaaagt ggtaccggca gaaatcagac ggtctgcttc
    37201 aatcatcctt tacctcataa cgcggcgcgt agggcttcgc gaattaatgt ttcactgctg
    37261 gcgtcagggc gagcgatttt gctcaccatg cggcttgcct cttgtggttt atagcccagc
    37321 gccaccagcg cggcaactgc ttcttgttca gcatcgtcgg tcgccgggct ggcaggtgat
    37381 gtgagtacca ggtcggcggc tggcgtaaag agatcgccat gcaaaccttt aaatcggtct
    37441 ttcatttcaa caatcaagcg ttcggcggtt tttttgccaa tacccggcag tttcaccagc
    37501 gcccccactt cttcacgctc aacggcatta acgaactgct gcgctgacat tccggagagg
    37561 atcgccagcg ccaacttcgg gccgacgccg ttggttttga tcaactcttt gaacaatgtg
    37621 cgctcttgtt tattgttaaa accgtacagc agttgcgcgt cttcacgcac cacaaagtgg
    37681 gtgaaaacga tcgcttcctg acccgcttca gggagttcat aaaaacaggt catcggcata
    37741 tgcacttcat agcctacgcc gcccacttca attaacacca gcgggggttg tttttcaatg
    37801 atgatgcctc tgagtctgcc tatcacatga cgctcctgcg taatgaatca aagataatgc
    37861 tgtatgataa aaaaatgctg gatagatatc cagcgaagga tgaagaaaac ttgcgaggtg
    37921 tcccgatgat ctgaaaaatg
//
LOCUS       DAFNBJ010000025        37876 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000025 DAFNBJ010000000
VERSION     DAFNBJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.025"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(290..1231)
                     /gene="fabY"
                     /locus_tag="L2X49_001284"
                     /gene_synonym="yiiD"
     CDS             complement(290..1231)
                     /gene="fabY"
                     /locus_tag="L2X49_001284"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBN4796396.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1276..1713)
                     /gene="dtd"
                     /locus_tag="L2X49_001285"
     CDS             complement(1276..1713)
                     /gene="dtd"
                     /locus_tag="L2X49_001285"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBN4796397.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1710..2582)
                     /locus_tag="L2X49_001286"
     CDS             complement(1710..2582)
                     /locus_tag="L2X49_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBN4796398.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2576..3175)
                     /gene="yihX"
                     /locus_tag="L2X49_001287"
     CDS             complement(2576..3175)
                     /gene="yihX"
                     /locus_tag="L2X49_001287"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBN4796399.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3274..4059)
                     /locus_tag="L2X49_001288"
     CDS             complement(3274..4059)
                     /locus_tag="L2X49_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBN4796400.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4093..4989)
                     /gene="yihV"
                     /locus_tag="L2X49_001289"
     CDS             complement(4093..4989)
                     /gene="yihV"
                     /locus_tag="L2X49_001289"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBN4796401.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5157..6053
                     /gene="yihU"
                     /locus_tag="L2X49_001290"
     CDS             5157..6053
                     /gene="yihU"
                     /locus_tag="L2X49_001290"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBN4796402.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6077..6955
                     /gene="yihT"
                     /locus_tag="L2X49_001291"
     CDS             6077..6955
                     /gene="yihT"
                     /locus_tag="L2X49_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBN4796403.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6972..8213
                     /gene="yihS"
                     /locus_tag="L2X49_001292"
     CDS             6972..8213
                     /gene="yihS"
                     /locus_tag="L2X49_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBN4796404.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8351..9253
                     /locus_tag="L2X49_001293"
     CDS             8351..9253
                     /locus_tag="L2X49_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HBN4796405.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9452..11488
                     /gene="yihQ"
                     /locus_tag="L2X49_001294"
     CDS             9452..11488
                     /gene="yihQ"
                     /locus_tag="L2X49_001294"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBN4796406.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPAGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            11534..12919
                     /locus_tag="L2X49_001295"
     CDS             11534..12919
                     /locus_tag="L2X49_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4796407.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQHVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            12962..14365
                     /locus_tag="L2X49_001296"
     CDS             12962..14365
                     /locus_tag="L2X49_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4796408.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            14433..15125
                     /gene="ompL"
                     /locus_tag="L2X49_001297"
     CDS             14433..15125
                     /gene="ompL"
                     /locus_tag="L2X49_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBN4796409.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(15216..16481)
                     /locus_tag="L2X49_001298"
     CDS             complement(15216..16481)
                     /locus_tag="L2X49_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4796410.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(16583..17563)
                     /locus_tag="L2X49_001299"
     CDS             complement(16583..17563)
                     /locus_tag="L2X49_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBN4796411.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(17571..18281)
                     /locus_tag="L2X49_001300"
     CDS             complement(17571..18281)
                     /locus_tag="L2X49_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBN4796412.1"
                     /translation="MAENQSTVENAKEKLDWWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(18498..20321)
                     /gene="typA"
                     /locus_tag="L2X49_001301"
     CDS             complement(18498..20321)
                     /gene="typA"
                     /locus_tag="L2X49_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBN4796413.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            20694..22103
                     /gene="glnA"
                     /locus_tag="L2X49_001302"
     CDS             20694..22103
                     /gene="glnA"
                     /locus_tag="L2X49_001302"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBN4796414.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22422..23471
                     /gene="glnL"
                     /locus_tag="L2X49_001303"
     CDS             22422..23471
                     /gene="glnL"
                     /locus_tag="L2X49_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBN4796415.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23483..24892
                     /gene="glnG"
                     /locus_tag="L2X49_001304"
     CDS             23483..24892
                     /gene="glnG"
                     /locus_tag="L2X49_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBN4796416.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            25004..25114
                     /locus_tag="L2X49_001305"
     CDS             25004..25114
                     /locus_tag="L2X49_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBN4796417.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25304..26677)
                     /gene="hemN"
                     /locus_tag="L2X49_001306"
     CDS             complement(25304..26677)
                     /gene="hemN"
                     /locus_tag="L2X49_001306"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBN4796418.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(26866..27375)
                     /gene="yihI"
                     /locus_tag="L2X49_001307"
     CDS             complement(26866..27375)
                     /gene="yihI"
                     /locus_tag="L2X49_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBN4796419.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVIEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            27957..28589
                     /locus_tag="L2X49_001308"
     CDS             27957..28589
                     /locus_tag="L2X49_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBN4796420.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(28971..31757)
                     /gene="polA"
                     /locus_tag="L2X49_001309"
     CDS             complement(28971..31757)
                     /gene="polA"
                     /locus_tag="L2X49_001309"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBN4796421.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            32121..33053
                     /locus_tag="L2X49_001310"
     CDS             32121..33053
                     /locus_tag="L2X49_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBN4796422.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFIGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(33094..34524)
                     /locus_tag="L2X49_001311"
     CDS             complement(33094..34524)
                     /locus_tag="L2X49_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBN4796423.1"
                     /translation="MIHKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(34679..35305)
                     /gene="dsbA"
                     /locus_tag="L2X49_001312"
     CDS             complement(34679..35305)
                     /gene="dsbA"
                     /locus_tag="L2X49_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBN4796424.1"
                     /translation="MKKIWLPLAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(35322..36308)
                     /gene="srkA"
                     /locus_tag="L2X49_001313"
     CDS             complement(35322..36308)
                     /gene="srkA"
                     /locus_tag="L2X49_001313"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBN4796425.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(36385..36654)
                     /locus_tag="L2X49_001314"
     CDS             complement(36385..36654)
                     /locus_tag="L2X49_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBN4796426.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            36724..37308
                     /gene="mobA"
                     /locus_tag="L2X49_001315"
     CDS             36724..37308
                     /gene="mobA"
                     /locus_tag="L2X49_001315"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBN4796427.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMVF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            37290..37817
                     /gene="mobB"
                     /locus_tag="L2X49_001316"
     CDS             37290..37817
                     /gene="mobB"
                     /locus_tag="L2X49_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBN4796428.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         9334 a         9620 c         9233 g         9689 t
ORIGIN      
        1 tagattttaa aaatatttat gagaaaagta tggcaccaga gggggttttg actatcaaaa
       61 atcgctcaaa cctgaactaa gatgatgagt taagcattat ctgcgataat tgccactcag
      121 ctctacaaat aatatttaaa tgctcctgaa cagtgatgta gcaagacacg tatggagcca
      181 acgaagaata aaaatttata gagttcttat tcaatataac cggaatagtc aggggctgat
      241 gtaaatgacc ggaatgagta agagaggcag caccgcccct ctccatcagc tactcttctt
      301 cgttcccgcc ctcttcatac gggccaaatg gcttcgcggg cagaacgata tacgtgcctt
      361 caaacactgc acccggcgtc tcgtcgccaa agatttcaac ttgcatctgc acccgtgctt
      421 ttcgtccgcg cgccagacgg tcgagatcgc cgcttaaggc accaaggtcg gctaccgcat
      481 gaggtttacc gctaatcggc ttgctgtagc ggatatgcgc atccgccaga ataatcgttc
      541 cgccgagatg gcgttcgcgc agcatcagcc agataagccc ccaaccggtg agcgttgcca
      601 gtgagaataa actcccggca aacagcgtat ggtgcggatt ctgattaccg gtttccggca
      661 tggtggtgat aaatttttgc ccggtatatt gttgaatacg cacgcccatt ttttcactaa
      721 gcgggatatg ttcgtaccac gcctgttgca gctgcgcgca ccagtcgccg cgatgcaaaa
      781 tatcatccag agtggcaacg ggtttaatca tcaaaaaatg gcgaatcggc gtggtagttg
      841 gggtggtgat ctccccctga ttaatgaatc ccagcttggc gaaaaactcc accgcgtctt
      901 cacgggcgct acaggtcacg cgctttacgc cttcctgacg cgccaccgac tccagggtca
      961 tcgccatcag cgtgcctaac cctttgtcct gcacgtccgg gtgaacggcc ataaagcgaa
     1021 tggacgcttc attgtcggca ttaatataca atcggcctac cgccaccaga ttaccctgct
     1081 cgtcgacgac catctggtga tgcgccatcg catcccacgc gtcgcgttcc gaaccttttg
     1141 gttgatgcag gggcttacgc aacatttccc agcgaaactg atagtaacgc tctaattctt
     1201 cttctgtttg tggaacccga aggtgataca tagcgatact ctctcttgtt tcccgtgaca
     1261 accctggaag ctggctcata cctgcaacca gaatgtcacg gggccatcat tgaccagcga
     1321 tacctgcata tccgcagcga agcgtcctgt ttgcgtgttc atctcttgct gacggcagcg
     1381 ttcgacgaaa tagtcatata acgcctctgc gcgatccggt gatgcacctt tggagaaact
     1441 tgggcgcatc ccccgttcgg tatctgcggc gagggtaaac tgggaaacca ccagcacact
     1501 gccgcccgcc tgttgcacgt tgaggttcat cttgccttcg gcatcgctaa agatgcggta
     1561 gccgagcaca cgctcgcaca gacggtttgc tttctgttcg tcgtcatcct tttcgacacc
     1621 caataacacc aaaagtcccg cgccaatttc gcccgtcact tctccctcca cggtgacgct
     1681 ggcacgggtt acgcgttgaa ttaatgcaat catggttcgt cgtcttcttc ttgttcagct
     1741 gcttgtttta gtttgcggta ttccccgaga gtgacagtaa tttccgcgcc aagcaagacg
     1801 atacaccatg tccagtagac ccaaacaaag agaatgggga tcaccgccaa cacgccgtaa
     1861 atgagctgat atgacggaaa catggtgata taaagcgcga aacctttctt tcctgcttcg
     1921 aacaggagcg cggcgacaaa cgcgccgaca atcgcgtcgc ggttaggtac gcggatggta
     1981 ggaacaatgc tgtacagcaa ccagaaggag atccacgaca acagcagcgg aaaaatacgc
     2041 agcacgttat cgatgacagt attgagatcg ctcgcccagc gcagggagag caaataggaa
     2101 ctgatcgcca gactggcccc tgccagcagc ggccccagcg ttaaaatcat ccagtacacg
     2161 gcgaacgagt aaatttttgg tcgcgctcgt ttactgcgcc agatggtatt caacgcgcta
     2221 tcgatggagt acatcagcaa taacgcggtg acgatcagcc cgcacgcccc aacggcggtc
     2281 atcttgttgg aattggcaac aaattgttcg atataccgct gaataacatc gccagtagca
     2341 ggcagaaagt tggcaaaaat aaagtgacgt aactgaatgc tgacgtcgga aaacatggga
     2401 aaagcggcga aaagcgcaaa aacaacggca accagcggca ctaatgagag caacgacaca
     2461 taggcaaggt tacctgccag ggttgtcatg ttgtcctcat caatgcgttg ccagagtagt
     2521 tttagccagg cccatagtgg acgggtacgg tgcctggctt tgtcctgaat ggtttttagc
     2581 ataacacctt cgcgaaatag tccgggatgg tggttttatc tttcaccaga atactggtaa
     2641 tgcccagctg attggctcct tctatattat cggcgttatc gtcgaaaaag accgtatcgc
     2701 tgggtgaaaa accttccgcc tgcaaaacat gctggtaaat tcgtgcttca ggtttgcgca
     2761 tccccagatc ttgcgacaga tagatatggt cagcagcatc acgaatttcc gggtattctt
     2821 ccggccagaa ggtggtatgc aggcggttgg tattggaaag caccaccacg cgatgcccct
     2881 gctcacgcag tttatgcatg atggcgatca cttccgggcg cagcgccaca aacaccgcct
     2941 gccagccgtg agagaactgc tcgtagctta gcggtagagc catctcatga cacagcgcct
     3001 ctgcgaacgc ttcgtcgcta atttccccac gctcatgctg atgaaacgcc tcccccatat
     3061 gaaaactctt cttaagcgat gccagcggaa tacgcgttaa atcgctccag gctcccagca
     3121 cacggttaaa gtcgatatcg acaatcacat tacctaaatc aaagatatag agcatttttg
     3181 cctcctttcg cgccacaaga aattaactgt agcgggaaag gatgggtttg gctatgtggc
     3241 aaggtatgaa gccaggttaa cggtcacaaa aatctacgcg tcttcctggg gaagaatgat
     3301 ttcaatttgg ctgtcttgta agcgtgattt tagcgcagcg ggcggcagct cgtcggtaaa
     3361 gagcgcagtg acctgtgcca cgttaccaat ttcaaccgcc gccgaagcat gatacttagt
     3421 gtgatcggcg accagcagaa tatttctcgc gtgcgccatc atcgttttca ccacgttagc
     3481 ttcgtttaca tcaaactcca tcaacgcgcc atcgctctca atcgccccaa cgcttgttac
     3541 cagataatca gcacgaaaat cagccacaaa ggacgccgct gaagggccaa tgatcccgct
     3601 attatgagag cgcaacgtac cgccgggcac catcacttca aagcgcgggt tgtggtaaag
     3661 aatatgcgcc acacgcaggc tgttggtgat tatccgcaaa tgattatggt taagtaacgc
     3721 ccgggcaaca tgctcaacag tcgtaccaat ggtgataaat attgttgaac catcaggaat
     3781 atagtctgcc accgcttcgg caatcgcttt tttttcctcg gtttgcgaaa cctcacgctg
     3841 ctcgaacgcc gtattaacga cgctggaagc ccgacccgcg ccaccgtgat ggcgcgtaat
     3901 caggccttgc tcgcttaatt tacgaatatc ccggcggacc gtctgcgtgg agacatccag
     3961 cagatttgcc agctcatcaa tattcatata cccacgctcg gcgatcagca tgaggagttg
     4021 gtcgtgccgc gggttaccgg ttagttcggt aaggctcatg aaaattcctc gaaaaaccat
     4081 caccctggca ttttatacaa aaagtgacaa aaaagatcgg gtttgatcac agtcagggat
     4141 cccggctcgt ccaccgggac gtgtgcattt taacgccgct acaccgctgg cgaagcggac
     4201 tgactccgct aaatccccac ttgttgccag cgccaccgcc aaagcgccgt gaaaaacatc
     4261 acccgcacct gtggtatcta ccacatcaac tttgaaggcc ggttgatgct gacgcccacc
     4321 attttccagc cagtcgcagc ctgcgctacc ctgggtcaca tagacatgac catttgtgag
     4381 cgtttgtgcc tgttttagcg cactggccat ctctttcacg cccgttaagc gcgccagacc
     4441 cggttctgaa aaggccgcgt gatcgcttaa tgccaccagc tcactgatat cctgcggcgt
     4501 aatgtccccg tccagaacgg tcatcacacc cgcctgacgg gccagggtga aggctttttt
     4561 agcgccgtcg tgccagcgta catctgccag cacaacatcc cactgagaga aatcaatttc
     4621 ctccaaccac tctgcgtcag gcagcagatc cgggctgggg tagttaatga ttatccgctc
     4681 gcctttggta tccaccatga tggcggattg cgaagatttc gcctggttat accgtttggt
     4741 gtaacgggtg ttaacgcccc aggattccag ctctgccagc aggctgttgc ccgtgtcgtc
     4801 atcacctacg cgaccaataa aatcgacctg cgcccccagc cttgccgccg caaccgctgc
     4861 cgtcgccgct ggcccgccac caacttccgt ataatttctc gccacgtatt taccgctctc
     4921 cgtcggtaac ccttccacgt aatagatgcg atccatcacg gttataccta cacaagcaac
     4981 acgaatcatg gtcattcctt aaacattttg ggatgcgttc attttaattt caccaattgt
     5041 ttaaaaagtg acggctgtca attttttgac gataaattac aaatacgatc aaaaacagac
     5101 aagcaaagca tgtcataaat gacaaaaagt gacatcactg tattcaggag agggttatgg
     5161 cagcaatcgc gtttatcggt ttaggacaaa tgggttcgcc aatggcgagc aatttattgc
     5221 agcaagggca tcaacttcgc gtctttgatg tgaacgccga agctgtgcgg catctggtag
     5281 acaaaggtgc gactcccgcc gccaacccgg cgcaggccgc taaagatgcc gaatttatca
     5341 ttaccatgtt gccgaatggc gatctggtgc gcaacgtgtt gttcggtgaa aacggcgttt
     5401 gcgaaggctt atctaccgat gcgctggtca ttgatatgtc caccatccat ccgctgcaaa
     5461 ccgataaatt gattgccgat atgcaagcca aaggcttcag catgatggat gttccggtag
     5521 gccgtacttc tgcaaatgcc attaccggta ctctgttact gctggctggc ggcaccgctg
     5581 aacaagttga acgtgccaca ccgatcctga tggcgatggg cagtgagttg atcaactctg
     5641 gcggtccggg catggggatc cgcgttaagc tcatcaacaa ctacatgagc atcgcgctca
     5701 atgcgctttc ggcagaagcc gccgttttgt gcgaagccct gaatcttccc ttcgatgttg
     5761 ccgtcaaagt gatgagcggt accgccgccg gtaaaggtca cttcaccact tcctggccaa
     5821 acaaagtcct cagcggagat ctttctcccg ccttcatgat cgatcttgcc cataaggatc
     5881 ttggcatcgc ccttgatgtc gccaaccagc tgcatgtgcc aatgccgctg ggggccgcct
     5941 cacgggaggt ttatagccag gcgcgcgcag cgggtcgcgg tcgccaggac tggtccgcca
     6001 ttctggaaca ggtccgtgtc agtgccggga tgactgccaa agtaaaaatg taacgactgg
     6061 ataaaggaat aaatgaatga ataagtacac catcaacgac attacgcgcg catcgggcgg
     6121 ttttgccatg ctggcggtcg atcagcgcga agccatgcgc atgatgtttg ccgcggctgg
     6181 ggcacccgct ccggtagccg atagcgtttt aactgatttc aaagttaacg ctgcaaaggc
     6241 cctctcgcct tatgcctcgg cgattctggt agatcaacaa ttctgctacc gccaggtggt
     6301 tgagcaaaac gcgattgcca aaagttgcgc catgattgtc gccgccgatg agttcattcc
     6361 tggcaacggt attccggtcg atagcgtggt tattgaccgc aaaatcaatc cgctacagat
     6421 caaacaagac ggtggcaaag ccttaaaact gctggtgctg tggcgtagtg atgaagatgc
     6481 gcagcaacgt ctggatatgg tgaaagagtt caacgaactg tgccactcac acggtctggt
     6541 aagcatcatt gagccagtcg tccgcccacc gcgtcgtggc gataaattcg atcgcgaaca
     6601 agcgatcatc gatgccgcca aagagctggg cgacagtggc gctgacctct acaaagttga
     6661 aatgccgctt tatggcaaag gtccgcaaca agagcttctc tgtgcttcac aacgtctgaa
     6721 tgaccatatc aatatgccat gggtgatcct ctcttccggt gtcgacgaaa aactgttccc
     6781 gcgtgccgta cgcgtggcaa tgacggcggg cgcatcggga ttcctggcag gccgtgcagt
     6841 ctgggcatcg gtcgtcggtt taccagacaa cgagctgatg ctgcgtgacg tttgcgcacc
     6901 gaaattacaa caacttggcg atatcgtcga cgaaatgatg gctaaacgcc gctaagatga
     6961 ggataaaagg aatgaaatgg tttaacaccc taagccacaa ccgttggctg gaacaggaaa
     7021 ccgaccgcat ctttgatttt ggtaaaaatt ccgtagtgcc gactggtttt ggctggttag
     7081 gcaataaagg gcaaatcaaa gaagagatgg gcacccatct gtggatcacc gctcgtatgt
     7141 tgcacgttta ttccgttgct gcggcgatgg gtcgacctgg cgcttactcg ttggttgatc
     7201 acggtatcaa agccatgaac ggcgcactgc gtgataaaaa atatggcggc tggtatgcct
     7261 gcgtgaatga cgagggcgtg gtggatgcct ccaaacaggg ctatcaacac ttctttgctc
     7321 tgctgggtgc cgccagcgcc gtcacaacgg gtcacccgga agcgcgcaag ctgctcgatt
     7381 acaccattga aattatcgag aaatatttct ggagcgaaga agagcagatg tgcctggaat
     7441 cctgggacga agccttcagc aaaaccgaag agtaccgcgg cggcaatgcc aatatgcacg
     7501 cggtggaagc tttcttgatt gtttatgacg tcactcacga caaaaaatgg ctggatcgcg
     7561 cgattcgcgt ggcttccgtg attatccacg acgtcgccag aaataatcat tatcgcgtta
     7621 acgaacactt cgatacccag tggaatccgc tgccggatta caacaaagat aacccggcgc
     7681 atcgcttccg cgcgtttgga ggtacaccag gccactggat cgaatggggc cgtttaatgc
     7741 tgcacatcca tgctgccctg gaagcccgtt gcgaacaacc accagcatgg ctgctagaag
     7801 atgccaaagg tctgtttaac gccaccgtgc gcgatgcctg ggcacccgat ggcgcggacg
     7861 ggattgttta taccgttgac tgggaaggaa aaccggtggt ccgcgaacgt gtacgttggc
     7921 ctatcgtcga agcaatgggt acggcctacg cgctctacac cgtcactggc gatcgccagt
     7981 atgaaacctg gtatcaaaca tggtgggagt actgcattaa gtacctgatg gactatgaaa
     8041 atggttcctg gtggcaggag ctggatgcgg acaataaggt caccaccaaa gtctgggacg
     8101 gcaaacagga tatttatcac ctgctgcatt gcctggtgat cccgcgtatc ccgttagcgc
     8161 ccggcatggc tccagcggtt gcagcgggtc tgctggatat taatgcgaaa taactgatct
     8221 gaaaataggg tgcgggtttt cccctcaccc taaccctctc cccaaagggg cgaggggacc
     8281 gtccggtgca gtttttgggt ttgacatcag cctcacggga ggctgtatgt cgttaattaa
     8341 ggtgccttgt atgcaaatca ccaatatgca ttgcagtgga cagaccgttt cacttgccgc
     8401 gggggattat cacgcgacca ttgtgaccgt cggtgctggt ctggctgaat taacctttca
     8461 gggttgtcat ttagtcatcc cgcacaaacc cgaagagatg ccgctggcgc atctgggtaa
     8521 agtgcttatt ccgtggccga accggatcgc caacggctgt taccgctatc aggggcagga
     8581 atatcagtta cccattaatg aacatagttc aaaagccgct attcacgggc tgcttgcctg
     8641 gcgagactgg caaattagtg aattaaccgc caccagcgtt accctgacag cttttctgcc
     8701 gcccagttac ggctatccgt ttatgctggc ttcacaagtt gtttattcgc tcaacgcaca
     8761 cacgggtctt tccgttgaga tcgcaagcca gaatatcggc actgtcgcag cgccttatgg
     8821 cgtgggtatt catacttatt taacctgcaa cttaacatct gttgatgaat atctgttcca
     8881 gcttccggca aaccaggttt atgccgttga tgaacacgta aatccgacaa cgcttcacca
     8941 cgttgatgag ctggatttaa atttcacgca ggcgaaaaca atagccgcga caaaaatcga
     9001 ccacaccttt aaaaccgcta atgatctgtg ggaaataacc attacccatc cacaacaggc
     9061 gctttcggtt agcctttgtt gcgatcaacc ctgggtacaa atttatagcg gtgaaaaatt
     9121 acagcgtcag ggactggcag ttgaacctat gagttgcccg cctaacgcct ttaattcagg
     9181 tatcgactta ttattactgg aaccaggaaa accgcaccgc ctgtttttta acatttacgg
     9241 acagagaaaa taacttgtat tgattagcag tcaaaatcat tgaagaacat ttcactcgct
     9301 aaaggcaaca ttatgtatct tcaatttatt aagttaaaaa cagctcagct tctgtaaagt
     9361 ttttatatat cagttttatg agaatcattt tacataagcc tgtaataaca ggaaggcaac
     9421 aatctcgttc tcaactctat ggagattagt tatggatacg ccacgtccac agttattaga
     9481 ttttcaattt catcagaata acgacagttt taccctacat tttcaacaac gtcttatttt
     9541 aacccatagc aaagataatc cttgtttatg gattggctca ggtatagcgg atatcgatat
     9601 gttccgcggt aatttcagca ttaaagataa actacaggag aaaattgcgc ttaccgacgc
     9661 catcgtcagc cagtcaccgg ctggttggtt aattcatttc agccgtggtt ctgacattag
     9721 cgccacgctg aatatctctg ccgacgatca gggccgttta ttgctggaac tacaaaacga
     9781 caaccttaac cacaaccgta tctggctgcg ccttgccgct caaccagagg accatatcta
     9841 cggctgcggc gaacagtttt cctacttcga tctgcgtggc aaaccgttcc cgctatggac
     9901 cagtgaacaa ggcgttggtc gcaacaaaca aacctatgtc acctggcagg ccgactgcaa
     9961 agaaaatgcg ggcggcgact attactggac tttcttccca cagcctacgt ttgtcagcac
    10021 gcagaagtat tactgccatg ttgataacag ttgctatatg aacttcgact ttagtgcccc
    10081 ggaataccat gaactggcgc tgtgggaaga caaagcaacg ctgcgttttg aatgtgctga
    10141 cacatacatt tccctgctgg aaaaattaac cgccctgctg ggacgccagc cagaactgcc
    10201 cgactggatt tatgacggag taacgctcgg cattcagggc gggacggaag tgtgccagaa
    10261 gaaactggac accatgcgta acgcgggcgt gaaggtcaac ggcatctggg cgcaggactg
    10321 gtccggtatt cgtatgacct cttttggcaa acgcgtgatg tggaactgga agtggaacag
    10381 cgaaaactac ccgcaactgg attcacgcat taagcagtgg aatcaggagg gcgtgcagtt
    10441 cctggcctat atcaacccgt atgttgccag cgataaagat ctctgcgaag aagcggcaca
    10501 acacggctat ctggcaaaag atgcctctgg cggtgactat ctggtggagt ttggcgagtt
    10561 ttacggcggc gttgtcgatc tcactaatcc agaagcctac gcctggttca aggaagtgat
    10621 caaaaagaac atgattgaac tcggctgcgg cggctggatg gctgacttcg gcgagtatct
    10681 gcccaccgac acttacctgc ataacggcat cagcgccgaa attatgcata acgcctggcc
    10741 cgcgctgtgg gcgaaatgta actacgaagc ccttgaagaa acgggcaagc tcggcgagat
    10801 ccttttcttt atgcgcgccg gttctaccgg tagccagaaa tactccacca tgatgtgggc
    10861 gggcgaccag aacgtcgact ggagtctcga cgatggcctg gcgtcggttg tcccggcggc
    10921 gctgtcgctg gcaatgaccg gacatggcct gcaccacagc gacattggcg gttacaccac
    10981 cctgtttgag atgaagcgca gcaaagagct gctgctgcgc tggtgcgatt tcagcgcctt
    11041 cacgccgatg atgcgcaccc acgaaggtaa ccgtcctggc gacaactggc agtttgacgg
    11101 cgacgcagaa actatcgccc attttgcccg tatgacctcc gtcttcacca ccctgaaacc
    11161 ttacctgaaa gaggctgtcg cgctgaatgc gaagtccggc ctgccggtta tgcgcccgct
    11221 gttcctgcat tacgaagacg atgcgcacac ttacaccctg aaatatcagt acctgttagg
    11281 tcgcgacatt ctggtcgctc cggtgcatga agaaggccgt agcgactgga cgctctatct
    11341 gccggaggat aactgggtcc acgcctggac gggtgaagcg ttccggggcg gggaagttac
    11401 cgttaatgcg cccatcggca agccgccggt cttttatcgc gccgatagcg aatgggcggc
    11461 actgttcgcg tcgttaaaaa gcatctaatc acatccgccc gtggggaaac tcatgggcaa
    11521 agggagaaca tcgatgagtc acatcacaac ggaagatcca gcaactttac gcctgccctt
    11581 taaagagaaa ctctcttacg gtattggcga cctggcctct aacatcctgc tggatatcgg
    11641 tacactttat cttttgaagt tttataccga cgttctgggg ctgccaggca cctatggcgg
    11701 cattatcttt ttgatttcaa aattctttac tgcgtttacc gatatgggta ccggcattat
    11761 gttggattcc cgacgcaaga tcggtccaaa aggtaagttc cgtcctttta ttctgtatgc
    11821 gtcattcccg gtcaccttac tggcgatcgc caactttgtc ggcacaccgt ttgatgtcac
    11881 cggtaaaacg gtgatggcca ctattctgtt tatgctttac ggactgtttt tcagcatgat
    11941 gaactgctcc tacggcgcaa tggttcccgc tatcaccaaa aaccccaacg aacgcgcctc
    12001 actggcggca tggcgtcagg gcggcgctac gctgggcctg ctgctgtgca cggtgggatt
    12061 cgtgccagtg atgaatctta tcgagggtaa tcagcaactt ggctatatct tcgccgccac
    12121 gctgttttca ctgttcggcc tgctgtttat gtggatctgc tactcaggcg tgaaagagcg
    12181 ttatgtcgaa acccaaccaa ccaatccggc gcaaaagcct ggcctgttgc aatctttccg
    12241 cgcaattgcc ggtaaccgcc cactgttcat tctgtgtatt gccaacctct gcaccttagg
    12301 ggcgtttaac gtcaagctcg ccattcaggt ctattacacc cagcacgtac ttaacgatcc
    12361 catcctgttg tcgtatatgg gatttttcag catgggctgt attttcatcg gcgtgttcct
    12421 gatgcctggc gcagtcagac gttttggtaa gaagaaggtc tatatcggcg gcctgctgat
    12481 ttgggtgctg ggcgatctgc tcaactattt cttcggcggc ggctcggtca gctttgtggc
    12541 gttctcctgc ctggcgttct tcggctcagc gtttgttaac agcctgaact gggcgctggt
    12601 ttccgacacc gtcgagtacg gcgagtggcg caccggcgtg cgttcggaag gcacggtcta
    12661 caccggcttc accttctttc gcaaagtgtc tcaggcgctg gctggtttct tccccggctg
    12721 gatgctgacg caaattggct atgtgccgaa cgtcgcacag gctgaccaca ccattgaagg
    12781 gttacgccag ttgatcttca tctacccaag cgcactggcg gtagtcacca ttgtggcgat
    12841 gggttgcttc tacagcctga acgagaagat gtatgtccgc attgtggaag agatagaagc
    12901 acgtaaacgc acggcgtaat aataataaac gacgccctgc ggggcgttat aaggagtgat
    12961 tatgtctgac cataatccac tgacattaaa actgaatctg cgggaaaaaa tcgcctatgg
    13021 tatgggcgac gtcggttcga atttaatgct ctgcatcggt actctgtatc tcctcaaatt
    13081 ttataccgat gaattaggga tgcctgctta ctatggcggg attatctttc tggtggcgaa
    13141 gttttttacc gcgtttaccg atatgctcac cggattttta ctcgactcgc ggaaaaatat
    13201 tgggcctaaa ggcaaatttc gtccctttat tttatatgca gcagttccgg cggcgttaat
    13261 tgcaacgctc cagtttatcg ccaccacctt ttgtttgccg gttaaaacga cgattgccac
    13321 cgcgctgttt atgatgtttg gactttcata cagtctgatg aactgctcgt atggtgcgat
    13381 gatcccggca attaccaaaa acccgaatga gcgcgcgcag ctcgcggctt accgccaggg
    13441 tggtgcaacc atagggctat tgatttgtac cgtggcgttt attccgttgc agtcgctttt
    13501 ttctgactca accgtcggtt atgcctgtgc ggcacttatg ttctccattg gcggctttat
    13561 ttttatgatg ctgtgctaca gaggcgtcaa agagcattat gtggacacag cgccaaccgg
    13621 acataaagcc agtattctca aatctttttg cgcgatattt cgtaatccac cattgctggt
    13681 tttatgcatt gctaacctgt gtactctggc ggcatttaat atcaaactgg cgattcaggt
    13741 ctattacacc cagtatgtgc tgaatgatat taatttattg tcgtggatgg gattcttcag
    13801 tatgggatgc atcctcgtcg gcgtattact ggtgccagta actgtaaaat gttttggtaa
    13861 aaaacaggtt tatttagctg gcatggtgct gtgggcggtg ggtgatatac tgaattattt
    13921 ctggggaagt aactctttca ctttcgtcat gttctcttgt gtcgcctttt ttggcacggc
    13981 ctttgtaaac agcctgaact gggcactggt accagatacc gtagattacg gtgaatggaa
    14041 aaccggtatt cgtgccgaag gttctgttta taccggttat accttctttc gtaaaatttc
    14101 tgccgcactt gctggcttct tgccaggcat tatgctgacg caaattggtt atgttcccaa
    14161 catcgcgcaa agcgatgcga cattacaggg tttgcgtcag ctcattttta tctggccttg
    14221 tgcactggca attattgctg cattgacgat gggattcttt tacacactca atgaaaaacg
    14281 gtttgcattg attattgagg aaatcaacca acgcaaaaat aaagagatgg cgacagaaga
    14341 aaaaacggct tccgtaacgt tataaatgtc aatagccgct atttccatcc tggtgggtgg
    14401 cggcctccct acgtttaaaa aatggactta ttatgaaaaa gattaatgca ataattttat
    14461 tatcttctct gacctctgcc tcggtatttg ctggcgcata cgtcgaaaac agggaggcat
    14521 ataatcttgc ctccgatcag ggcgaggtta tgctacgtgt gggttataac ttcgatatgg
    14581 gcgcgggtat tatgttaacc aatacctaca cctttcagcg agaagatgaa ctaaaacatg
    14641 gctataatga aattgaaggt tggtatcctt tattcaaacc aaccgataaa ttaaccatcc
    14701 agcccggtgg cttaattaat gataagagta tcggttcggg tggtgcagtg tatctggacg
    14761 tcaactataa atttgtacca tggtttaatc tgacagtacg aaatcgctat aaccataata
    14821 actatagttc aacagatttg agcggggaac tggataataa tgacacctat gaaattggca
    14881 cctactggaa ttttaaaatc accgataaat tttcctatac atttgagcca cattatttca
    14941 tacgcgtaaa tgactttaat agcagcaatg gaaaagatca tcactgggaa atcactaata
    15001 ccttccgtta tcgcattaat gaacactggc tgccttattt cgaattacgt tggttagatc
    15061 gtaatgtcga accgtaccac cgcgagcaaa accagattcg tatcgggacg aagtatttct
    15121 tctgatgtga aactgccgga tgcggcgtaa acgccttatc cggcctacgg ttgatacggt
    15181 tttcgttggc cggattaagc acatccggcc attaatcaag cttcagccgc cagcatctct
    15241 ttcttacgtt ggctaattcg cttaaccagc attacggaaa ccaccgcacc acagaatgcg
    15301 aagcaggcca taatgccaaa cacgattttg tagccaataa tccccggatt taaatccaga
    15361 atgtagccat acagactgaa gcagaacatc gccggagcgt aaccaataaa gctacccaac
    15421 gccatcgccg cgcctgtttt attttcagca atttttgctt cgccgatagg tgcaaaaaat
    15481 acggctcgct gtgtaaagac tatcgcgcca aagcccagcg tacatgccat ccctaaatag
    15541 accggcatac tttcgtgcgg cagcataatc aacagtacga gcgcagcggt actgatgata
    15601 aaggtgtagc atagatattt actcggcgat ttcaggatct tatctgaaat catgccacca
    15661 atcggtccac caatcatttt cagacagtat tggttaatga tgccgtaagc ccccaccagc
    15721 gcaacgggca atagatagat gtttttcagg aatggaatga agaatgtcag gccacagtaa
    15781 accgcgtaaa caaagaagac gttaaaagcg ataagccaga tggttttgtc tctcagcacc
    15841 gaggtcatac tggtgttttg cgatgctccg tcttcttttt tcacctcaac ggacggtgcc
    15901 tcttctttgt cattcaggac aaagaaaata ataatgccta cggcaatcac aataagggaa
    15961 tagaaccaga tgcctgcttt aaaacctaat aagccactgc caaaccaggt aaatactgcc
    16021 aacgcagaaa atgccaccac ggtatcgaca atgccacgcc ctgtttcgaa gaagccaaac
    16081 aaccgacctt gttgttcgct attgcccaat cgacttaccg acttgagcaa gaccggccag
    16141 ttcatcatgt cgcaagtaac gccaaatagc gcccagacaa agaggatgcc ccaatacccc
    16201 ggcattgtcg tcaaataaac acccagtaat cctgtcgcaa tgagtgagaa tgacatggtg
    16261 tatctgcgcg gtagtttatc ggcaaaataa atagacagaa aaaagcccac tgtggtgaca
    16321 aatgagttta ccgacatagc attaccaatt tgaccattgg tcaaatggaa atattcctgc
    16381 atcgggatat aaaacgcatc tttcagcgac ggtaatttat aaattgtacc gccacacagt
    16441 gttaatagac taaataacgc ccatttcttt ttcgtgagca taacatcccc taagcgattt
    16501 ttaaaatcac tgttttaaag gcaataagat accgaccatt catatagaat ggttttatta
    16561 actgcgtatg agttaaaact tattagtggt tcagaagatg gtttgcctct tgtttgattt
    16621 gttgtagtac gttacgcaca tgatttatct gattaattgc atcttctttt tctttacgta
    16681 accgcgcatc cagtaaatga ttttctggta ggggtgtttc actcatctgg cgataaggga
    16741 tccacggatt atcatcttcg tcttcaaagc ggaacgccgg cgcatagtaa tcaacctctt
    16801 cttccaggcc atatgccgtc acctgcggct catcatcacc aaggcagata aggtgcgtta
    16861 ataacgcttt gaacggcata tcaccctgac ctgaaataca ggctttatga cccaggccac
    16921 cctgttcttt aacgatcaag gcatctttaa tatggacctg ggtaatatgc ggtgccatcg
    16981 tttttaaggc gtcgatggga tgctcatttg cattgatcat gttcgcaaaa tcaaacagta
    17041 aggaaagaga ctccatctca ctttctttga ccagcgacac caactcatga ctttttaaat
    17101 cttcatgctg ctcgatcgta aaagtcaggc cgctgtcctg atacgtttcc cgtacatagg
    17161 caatgtcgtt agcgataatc gataatacgt cgcgcaaatt accttcataa cgtggataaa
    17221 aacgtacgga cgatgcccca gttttcaacg cgatggcgac ggcttcgtca atagatgcct
    17281 tatctgaggc gctggtttca atatgaatat caaggttcag acggcgggct ttatccccaa
    17341 aggcagagag ttctttatcg tccatattac caagagaaaa acgctcgcca tccagaacat
    17401 ggattttgac gccacgtaga ttattttcac ttgcgatatc aagtaaatca gcaggcaaca
    17461 ctctttccag ccgcatattt aaatgaaagg cataagcatg taaataaaga ggaagagtgt
    17521 cgacacgttg tagaatcttt cttgcgttat ttattgtcac cataaaacac ctacagatta
    17581 atgatgaggt ctacggcctc aaatcgccaa tattcaatat ctatctggac aatattgcct
    17641 tgctggtcgg cgcgatgctt ttcaattaag atcgctggca tacccgcaga accgccaaga
    17701 tacttgctga tatcgcccgg cattctgacg ggtttgaaaa tcaatttttt tactaccgtc
    17761 tctttttggt agcacttttc ccagactgca gaaaatgagt ggttttcaag ttgttcaata
    17821 aaacccggag caacttcagg attaatatat gtttcgtgat agaaaacttt atgtccttcc
    17881 agcgcccccc agccggtgat gcgatagagt tcagttgatg gtgtcactgc aatcagtggc
    17941 gcgagcgtct tgggaatatc gctggtacgg tttttctcgg taaaccccca ggaaggctct
    18001 cgtccttgct caattgcggc ccgctgaaag ctcgccgaca actccggact gtaattaaat
    18061 cgcggctggg tcacgaacca ccccttacga tccttacgga agattttgga ttctgcctcg
    18121 aggttcaaca acgcctggcg cagcgtcata cgtttaatgc ccagcagttc tcccagctct
    18181 ctttctgaag ggagttttcc acccggcgtg gtgatgccat ctttcaacca ccaatccagt
    18241 ttctcttttg cattttctac ggtggattga ttctccgcca ttgtttaatc cttttggttt
    18301 aaaccagatg aagcatctta cgcttacgcc aaaaaagaaa gcaattaacg gcgtatgact
    18361 ttgcagataa ttgtgacaaa gctctctatt gacgtaatcc cagacttata agggagatac
    18421 tcgcattggt gtgcgctcac gacaggtaaa aaaaaacctg ccagcgatgg caggtttttt
    18481 ataactaaaa aacgaaatta atcgtctttc ggtgcgcggt tggcgcgacg acgatcgttt
    18541 tcagtcaggt gacgtttacg aatacggata gaggtcggag tcacttctac cagttcgtca
    18601 tcatcgatga actccagagc ttgttccaga gtcatgcgga taggcggaac cagaacaacg
    18661 gcttcgtcag taccggaagc acgcatgttg gtcagtttct taccagtcag gcagtttacc
    18721 gtcaggtcgt tagagcggct gtgaataccg ataatctgac cttcgtaaac ttctgcaccg
    18781 tgaccgagga acagcttacc gcgatcctgc agaccgaaca gcgcgaacgc gaccgcttta
    18841 ccctgaccgt tagagatcag tacgccgttc tgacgctgac ccacttcacc cggacgtacg
    18901 tcgtcgtagt ggctgaaggt ggagtacagc agaccagtac cggaagtcat ggtcatgaac
    18961 tcagaacgga agccaatcag accacggctt gggatcacgt agtcgagacg tacgcggcct
    19021 ttaccgtctg gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc ctgcattaca
    19081 gaaccctgat gctgttcttc aacgtccaga gtcacgtttt catacggctc ttgtttacga
    19141 ccgtcgattt cacggaagat aactttcgga cgggataccg ccagttcgaa accttcacga
    19201 cgcatgtttt cgatcagaac agacaggtgc agttcgccac gaccagaaac gcggaacgca
    19261 tcggcgtctt cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc tttgttcaga
    19321 cgatccagga tctgacgaga cgttacgaac ttaccttctt taccgcagaa cggcgaggtg
    19381 ttaacgcaga agaacataga aacggtcggc tcatcaacgg agagtgccgg cagcgcttca
    19441 acgttttgcg tgtcgcaaac ggtgtcagaa atgttcagtt cgccaaggcc cgtgatcgca
    19501 acgatatcgc cagcttccgc cagatcggtt tcgatacgtt ccagaccgag gtggcccagc
    19561 actttaccga ctttcgcgtt gcgggttttg ccttcgctat cgatgatagt gacctgctgg
    19621 ttcggcttca ctttaccgcg cttgatgcgg ccaatgccga taacgccaac atagctgttg
    19681 taatcgagct gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc cggcgcagga
    19741 acgtgatcaa caatcgcctg atacagcggg gtcatgtctt ccgccatatc ttcgtggtcc
    19801 agacccgcga taccgttcag cgcagaagcg taaacgatcg ggaagtccag ctgctcgtcg
    19861 gtcgcgtcga ggttaacgaa cagatcgaat acctgatcca caacccaatc aggacgcgcg
    19921 ccagggcggt caactttgtt gataacaaca atcggcttca ggccgtaagc aaacgctttt
    19981 ttggttacga agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac cagcagcact
    20041 gagtctacca tggacattac acgttcaact tcaccaccga agtcggcgtg ccccggggta
    20101 tcaacgatgt tgatacggta atcattccat ttgatagcgg tgtttttcgc gaggatggta
    20161 atcccacgct ctttctccaa atcgttggag tccatcacgc gctcttgggt ttcggcacga
    20221 gagtcgaacg taccggattg ttggagcagc ttgtctacca gggtggtttt accatggtct
    20281 acgtgcgcga tgatggcgat attacgcaat ttttcgatca caactttgcc tcaggcatta
    20341 gaaatagcgc gttattgtac acggattaat cgcactacaa aacaggatca caaacatcct
    20401 ccgcaaacaa gtattgcaga gtccctttgt gatcgctttc acggagcata aaaagggtta
    20461 tccaaaggtc attgcaccaa catggtgctt aatgtttcca ttgaagcact atattggtgc
    20521 aacattcaca tcgtggtgca gcccttttgc acgatggtgc gcatgataac gccttttagg
    20581 ggcaatttaa aagttggcac agatttcgct ttatcttttt tacggcgaca cggccaaaat
    20641 aattgcagat ttcgttacca cgacgaccat gaccaatcca ggagagttaa agtatgtccg
    20701 ctgaacacgt actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca
    20761 ccgatactaa aggtaaagaa cagcacgtca ctatccctgc tcatcaggtg aatgctgaat
    20821 tcttcgaaga aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg
    20881 agtccgacat ggtgctgatg ccagacgcat ccaccgcagt gattgacccg ttcttcgccg
    20941 actccaccct gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc
    21001 gtgacccgcg ctccattgcg aagcgcgccg aagattacct gcgttccact ggcattgccg
    21061 acaccgtact gttcgggcca gaacctgaat tcttcctgtt cgatgacatc cgtttcggat
    21121 catctatctc cggttctcac gttgctatcg acgatatcga aggcgcatgg aactcctcca
    21181 cccaatatga aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg
    21241 ttccgccggt agactctgct caggatattc gttctgaaat gtgtctggtg atggaacaga
    21301 tgggcctggt ggttgaagcc catcaccacg aagtagcgac tgctggtcag aacgaagtgg
    21361 ctacccgctt caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgttg
    21421 tgcacaacgt agcgcaccgc ttcggtaaaa ccgcgacctt tatgccaaaa ccgatgttcg
    21481 gtgataacgg ctccggtatg cactgccaca tgtctctgtc taaaaacggc gttaacctgt
    21541 tcgcaggcga caaatacgca ggtctgtctg agcaggcgct gtactacatt ggcggcgtaa
    21601 tcaaacacgc taaagcgatt aacgccctgg caaacccgac caccaactct tataagcgtc
    21661 tggtcccggg ctatgaagca ccggtaatgc tggcttactc tgcgcgtaac cgttctgcgt
    21721 ctatccgtat tccggtggtt tcttctccga aagcacgtcg tatcgaagta cgtttcccgg
    21781 atccggcagc taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgatggta
    21841 tcaagaacaa gatccatccg ggcgaagcca tggacaaaaa cctgtatgac ctgccgccag
    21901 aagaagcgaa agagatccca caggttgcag gctctctgga agaagcactg aacgaactgg
    21961 atctggaccg cgagttcctg aaagccggtg gcgtgttcac tgacgaagca attgatgcgt
    22021 acatcgctct gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat ccggtagagt
    22081 ttgagctgta ctacagcgtc taatagttga agttgtacta cccggcgcaa caacgccgga
    22141 ggttcaaacc aggcccatct ggcgtaattg ttgcagccag tttgaacacg gacagcgcgc
    22201 agaacccgga gcgtacactg gtacgtgagg agttcgagca ctgcccaggt tcaaaatggc
    22261 aaataaaata gcctgatggg actggttttt agttgccgtg gaaatttcag cccatctctg
    22321 catgggcttt tttctccgtc aattctctga tgcttcgcgc tttttatccg taaaaagcta
    22381 taatgcacta aaatggtgca acctgttcag gagactgctt tatggcaaca ggcacgcagc
    22441 ccgatgctgg gcagatcctc aactcgctga ttaacagtat tttgttaatc gatgacaacc
    22501 tggcgatcca ttacgccaac cctgccgcgc aacaactgct cgcccaaagc tcccgcaaat
    22561 tgtttggtac accgttaccg gaactgttga gctacttctc attaaatatc gagctgatgc
    22621 aagaaagtct ggaggcgggg caaggtttta ccgataacga agtgacgctg gtcatcgacg
    22681 ggcgctcgca tatcctttct gtgacggccc agcgtatgcc ggacggcatg atcctgctgg
    22741 agatggctcc gatggataac cagcgccgct taagtcagga acagctacag cacgcccagc
    22801 aggttgctgc ccgtgattta gtgcgcggcc tggcacatga gattaaaaat ccgcttggcg
    22861 gtttacgtgg cgcggcgcag ctactcagca aagcgttacc tgacccatca ctactcgaat
    22921 ataccaaagt gattatcgaa caggcggacc ggctgcgaaa tctggtcgac cgtctgttgg
    22981 ggccgcagct gcccggtacg cgcgttaccg aaagtattca caaagtagct gaacgcgtgg
    23041 taacgctggt gtcgatggaa ctgccggaca acgtgcggtt gattcgtgat tacgatccca
    23101 gcctgccgga actggcgcac gacccggatc aaattgaaca ggtcttgctg aatattgtgc
    23161 gcaatgcgct acaggcgctg gggccggagg gtggtgaaat cattctgcgt acccgcaccg
    23221 cgtttcaact gaccttacac ggcgagcgct accggctggc ggcgcggatt gatgtggaag
    23281 ataacgggcc gggcattccg cctcatttgc aggatacgct gttttacccg atggtcagcg
    23341 gccgcgaagg tggcaccggg cttggcttat ccatcgcccg taatttgatt gatcagcatt
    23401 caggcaaaat tgaatttacc agttggccag gtcataccga gttctcggtt tacctgccta
    23461 tcaggaaata aaggtgacgt ttatgcaacg agggatagtc tgggtagtcg atgacgatag
    23521 ttccatccgt tgggtgcttg aacgtgcgct cgctggagcg ggtttaacct gtacgacatt
    23581 tgagaacggc gcggaagtac tggaggcgct ggcgagcaaa acgccggatg tgctgctttc
    23641 agatatccgt atgccgggaa tggacgggct ggcgctgctc aagcagatta aacagcgcca
    23701 tccaatgctt ccggtcatca ttatgaccgc acattccgat ctggatgctg ccgtcagcgc
    23761 ctatcaacaa ggggcgtttg attatctgcc caaaccgttt gatatcgacg aagccgtcgc
    23821 gctggttgag cgcgccatca gtcattacca ggaacagcag cagccgcgta atattcagct
    23881 taacggccca acgaccgata tcatcggcga agcgccagcc atgcaggacg tgttccgtat
    23941 tatcggtcgg ctttcgcgtt cttctattag cgtgctgatt aacggcgaat ccggcaccgg
    24001 taaagaactg gtcgctcatg ccctgcatcg ccacagtccg cgcgccaaag cgccgtttat
    24061 cgcgctgaat atggcagcta tcccaaaaga tttgatcgaa tcagaactgt ttggccacga
    24121 gaaaggcgcg tttactggcg cgaataccat tcgtcagggg cgttttgaac aggccgatgg
    24181 cggtacatta ttcctcgacg aaattggtga tatgccgctg gatgtgcaga cgcgtttgct
    24241 gcgcgtgctg gcagacggtc agttttaccg cgttggcggc tatgcgccgg tgaaagtgga
    24301 tgtgcggatt atcgctgcca ctcaccagaa tctcgaacag cgagtgcagg aaggtaagtt
    24361 ccgtgaggat ctgttccacc gcctgaacgt tatccgcgtt catctgccgc cgctgcgcga
    24421 acgtcgggaa gatattcccc gtctggcgcg ccatttttta caggttgccg cgcgcgaact
    24481 gggcgtagaa gcgaagttac tgcatccgga aaccgaagct gctctgacgc gtctggcgtg
    24541 gccaggcaac gtgcgccagc tggaaaacac ctgccgctgg ctaacggtga tggccgccgg
    24601 gcaggaagtg ttgattcagg atttgcccgg cgaactgttt gaatcaacgg ttgcggagag
    24661 tacttcgcaa atgcaaccgg acagctgggc gacgcttctt gcgcagtggg cagacagagc
    24721 gctgcgttcc ggtcatcaaa atctgctttc cgaagcgcag ccagagctgg agcggacgtt
    24781 actgacgacc gcgttgcgac atacgcaggg gcataaacag gaagcggcgc ggctactcgg
    24841 ctggggccgc aacaccctga cgcgtaagtt aaaagagctg gggatggagt gattcacggc
    24901 gtgtgtgtaa agattgatta ttgagcgcaa attgctggta ttttacgctt tactgttccg
    24961 ataagttcag tatgatcttg cccggaaaac ggggagagtc attatgctgg aatcaataat
    25021 taatctggta tcgagtggcg cagttgacag ccacacaccg caaactgccg ttgctgccgt
    25081 gctgtgtgcc gcaatgattg ggctgtttag ttgagtttca gattgctgat gaagtgctct
    25141 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaagcaa tattttcaac
    25201 aggttttgta ggcctgataa gcgtagcgca tcaggcaatc ttgttctcag cacattgatg
    25261 gcgctttcgt tttctacttt gtaaacgaag cgccattcac tatttaaatc acccgtgaga
    25321 actgctgcat ccgcgctttc tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga
    25381 tcagcaagcg acctttcgcc gtcacctgta ttcccttctc atccacatcc accagcccat
    25441 cttttgctaa cggggcgagc agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat
    25501 cccactgttt ctcaataggg gcgtaatcca gacggaagtt gcagatgagc gacttaatca
    25561 catcgcggcg aatacagtca tcacgcgtta gcgcaatacc acgccacagc gcattgcctt
    25621 gttcatccac ttgctgatag tactgcttca actctttctg gttctgcgcg tagcagtcgc
    25681 caatcatgct gatggcggaa acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt
    25741 agccctggaa gttacgatgc agcacgcctt cacgctgggc caccgccagc tcgtcatccg
    25801 gacgggcaaa gtgatccata ccgataaact gatagcccga ttgcgtcagg aaggcgatgg
    25861 tttcctgcag gatatcgagt ttttgctgcg gactcggcag gtcagcatct ttgattttgc
    25921 gctgagcagc aaaaatggtc ggcagatgcg cgtagttaaa gacactcaga cgatcggggt
    25981 tcagctccgc cacacgtttc agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc
    26041 cgtaaatcag gtcgatgttg gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg
    26101 caaagatgaa ctcttcatcc tgctcgcggt taaccagacg ttgcacttct ttgttgaagt
    26161 cctgcacgcc catgctcagg cgattaaagc cttcggcgcg taaatgatcg agtacatcca
    26221 gttcgatttc ccgcggatcg acttcgatcg aaatctccgc atcggcattg aactggaagt
    26281 tttcgcgcag cagcttcatc aggcggctga tttgcgcttt attcagatac gtcggcgttc
    26341 cgccgcccca gtgcaattgg ctgacgtgac gcccggcgaa cagcggtgca cgatggacga
    26401 tttcttgctc cagcgcgtcc agatactgat cggccttgtg ctgctggcga gtaacaatct
    26461 tattgcaacc gcagaagtaa caaagcttat ggcagaacgg gatatgtacg tagagagata
    26521 atggacgctc aggatagcgc gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg
    26581 aaaactccag cgcggtcggg tacgaggtgt atcgtggccc ggaatagtta tatttctgga
    26641 tcagggccag atcccagtcg atttgctgta cagacatgct cactccttcc gatggcgtct
    26701 ctggcggcta cggcgtaccg gcggatgccc gtttcgggtc atcagccggt tgcgcagcca
    26761 cttttgtcgc cgcgacaacc gctgtagttt aacgaataac cacaccagat aacatataac
    26821 cggaagggtt atcagcagaa cgggaaggcc cacggcggca atccgttagt tgccccgcag
    26881 cagacgcatc atgtcttctt gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga
    26941 gaggccgagt ttctgcatca actcatcaat acgatccagt ttagcatcca cccaggattg
    27001 ctcttcggcg ctcagggttt cgcctgcttc cagacgttcc agcagcgcat ccagacgctc
    27061 atccgtttcc agtaattcca actccgcctg cggtgaaagc ataggtttct cactcttcgg
    27121 tttgtgctgt ttggtgactt tttcaatcac gcccaatgga ataggggttt tactgccaat
    27181 acgtggatct tttggtgcgt tctggccttt gctgcctgac gtggtgtttc cgcccgctgc
    27241 gcggctgccc ggcgcatgac cacgacgttt tttctgacgc ttgcgatcac gagcttcctg
    27301 atccagctcc tcgcgtgttt ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga
    27361 agatgatggt ttcataatgg ttcgtctttt agccgtttta ttcagtatag atttgcggcg
    27421 gaatctaaca gaaagcaagc aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc
    27481 tgactcataa cccttaacgg gtcttacaat ccttgcaggc cgccaacaca ccctgttttt
    27541 cctttagccg gaacaatccg tgttgattcc atttccgttt aattacgtct ccggacgttt
    27601 gtcttcctga caatcctctg tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat
    27661 tgttcctgtt agcgtcctcg ctctatgggt gctactttac gcctttgctt taaacaaaca
    27721 atcaaccgcc atcagtattt gcacattttc agagttttat ataaaagcaa ttcactgatt
    27781 tatatagctt atttgacatg catcgcagaa ttttctctgc tatttcgcat aaagactatc
    27841 ggcaatgtct cacaaagcgt atggctttta cttacaacgc tcacgccgaa aaatccgcct
    27901 tttattatgg ttcaggtata atcgccacaa agctttgact gacggagacg accgctttga
    27961 ctaatttgaa ttatcaacag acgcattttg tgatgagtgc gcctgatatt cgccacctac
    28021 cttccgatac cggaattgaa gtggcttttg caggccgttc caacgcaggt aaatccagcg
    28081 cgctgaacac gctgactaac cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca
    28141 cccagcttat caacctgttt gaagtggctg acggcaagcg tctggttgac ttgcctgggt
    28201 acggttatgc ggaagtcccg gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat
    28261 acctcgaaaa acgtcagagc ctgcaaggtc tggtggtgct aatggatatt cgccatccgc
    28321 tgaaagattt ggatcagcag atgattgagt gggcggtaga cagcaatatc gccgttctgg
    28381 tgctgctgac caaagcggac aaactggcaa gcggcgcacg taaagcgcaa ttgaatatgg
    28441 tgcgtgaagc tgtactggcg tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga
    28501 agaaacaagg cgtggacaag ctgcggcaga aactggatac ctggtttagc gagatgcagc
    28561 ctgtagaaga aacgcaggac ggcgaataat tttcttgcct taatgcttgt gccggatgtg
    28621 gcgtatccgg cccgtaaatt caatagatta caattctacc gttggcctga taaacatagc
    28681 gcatcaggca ttacggatct tttctttcgc ccaataaaaa acgccccagt cattactgac
    28741 tggggcggct aaaatattca gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag
    28801 aacatcttac ctctgtaccc tacgcgacta actttactct tttttgttca gttcagaaag
    28861 cactttttgt aatttttttt cagttgttgc ataggaattt ctaatagcca tcacaaaacg
    28921 tgacagctta tgttgcttac ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc
    28981 tgatcccagt tttcgccact ccccacttcc accagcaacg gcacatccag acgggtacag
    29041 ttttccatca gttgatgaat ctgcttcgcg acggcatcaa catcatcttt atgaacttca
    29101 aataccagtt catcgtgtac ctgcatgatc atacgtacac gcggttgctc agcctgtaac
    29161 cacgcatcaa cggcaatcat cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc
    29221 gcgttaatgg ctgcacgttc agccgctgca cgacgagcac cattgctgga tttgatatcc
    29281 ggcagataca gacggcgtcc gtccagcgtt tcaacgtagc cctgctcttt cgcctgagca
    29341 cgggtgcgtt ccatatactc cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac
    29401 ttctgcgctt ctttacgtgg aatgttcaat tgccgcgcca gaccgaaagc actcatgcca
    29461 taaatcagac caaagttgat cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc
    29521 agtggcaaac caaacacttc tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat
    29581 gcggtcagca agcctttgtc acgcgagaga tgcgccataa tgcgcagttc aatctgcgag
    29641 tagtccgctg agacaatcac ataatcctct ggcgcaataa acgcctggcg gatacgacga
    29701 ccttcttcgt tacgcaccgg aatgttttgc aggttaggat cggttgacga taaacgtccc
    29761 gttgcggtta ctgcctggtg ataagaggta tgcacacgcc cggttttcgg gttgatcatc
    29821 agcggcagct tgtcggtgta agtcgacttc agcttcgcca gaccacgata ctccagaatc
    29881 acttttggca acggatagtc cagcgccagt tcttccagta cctcttccga cgttgacggc
    29941 gcgccacccg gcgttttctt cagcggttta ataccctgtt tttcaaacag aatggtttgc
    30001 aactgcttgg tggaagaaag gttaaattct tcacctgcaa tttcatgcgc tttcttttcc
    30061 agctcagcca gacgcaaggt gagctcttca gaatgattgt gcagcacttt cggatcgatc
    30121 ttcacaccgt tacgttcaat gcgtgaaagc actggcacca gcggcatttc gatattctcg
    30181 aagacgttca acggcccttt gtgtttttgc agatccggcc acattttcag atgcaactgc
    30241 aaggtgacat cagcatcttc ggcggcgtaa cgtccggcct cttcgagggc aatctggtta
    30301 aaggtcagtt gatttttgcc tttacccgca atctcttcaa aagtgatggt tttgtgcttc
    30361 aaccaacgtt ccgcgaggct gtccatatcg tgacgcccgg caacgctatt gagaatgtag
    30421 gactccagca tggtatcaaa cgcaatccca cgcagctcaa tgccgtaatt cgccagaata
    30481 ccgcgatcgt atttcaggtt ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt
    30541 tttagcaact cgagtgcacg ctcgcgagag atttgatcgg gcgcgtcaag ataatcatga
    30601 gcaaccggaa tatatgccgc tacgcctggc tcgatagcaa aagaaagccc gaccaggtta
    30661 gcagagatgt tatcaaggct gtcggtttcg gtatcaaatg caaataccgg cgctttttcc
    30721 agcttcgcaa tccacgcttt cagtgtttct tcatcaagga tggtgacgta gttgtcataa
    30781 gaaatcaccg ttgccgtcac ttctggcgct tcgtctgcaa cactggtttc ctgcggcttc
    30841 gcggctggtt ttgccccttt ggcctgtaac catttgcccg cttcgacatc agcagtccag
    30901 cgtttgaact catacttttt gaacagcccc aacaactctt ccgctgccgg ttgctgcact
    30961 tccagttgtt cacaggtcag ctccagttca acgtcggttt taatcgtcgc cagctggtat
    31021 gagagataag caacttcttt gttttgctcg agcttcgctg ccattgtttt cgcgccacgg
    31081 aagctcaacc cagcaatttt ttctggctcg gcatacagcg tatccagccc gccaagacct
    31141 tgcagcaatg cctgcgcggt tttttcaccg acgcccggta cgccaggaat gttatcggag
    31201 gagtcaccca tcagcgccag gaaatcgatg atcagttctg gcggcacgcc gtacttattc
    31261 accacctctt ccggtccgag gatggtattc gtcatggtat tgataagcgt aatatttggc
    31321 gtcaccagct gcgccatatc tttatcgcca gtgctgatca gcaccggacg cccggctttt
    31381 tcggcttcgc gcgccagagt accgataacg tcgtccgctt ctacgccaga aaccgccagc
    31441 agcggcagtc ccatcgcttt aaccatcgcg tgcaggggtt cgatttgtgc tcgcagatcg
    31501 ttcggcattg gcgggcgatg tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt
    31561 cccttggcgt caaagaccac cgctgcatgc gtcggtttat attgcatgat cagactgcgc
    31621 agcatgttga ggacaccata catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg
    31681 ggaaacgcgt gatatgcgcg ataaagataa gatgaaccat ctacaaggat aagtggattt
    31741 tgggggatct gaaccataat gtccgtgcct gtttatcaga ttatgggtaa aggatgccac
    31801 agacaggatg aaaacatgag tttttcaccg cttttcgtga aaatcttttg tagatcttct
    31861 ggatcgctcg caaatcgtca tgtggataac tttgtgcata aattatttac atcgctttta
    31921 atttattatc atgcttttta cagcatgatg tttgtctttg tttttcatgc agttaaaaat
    31981 aaagccatcc ctaataattg agataatggg attttttcta atgtggatag atatgaatta
    32041 tttttctcct taaggatcat ccgttatttg ggtcgttttc tgctaaaatc tgcgccttgc
    32101 gcttctttag cgcactgtct atggctaacc ttttgaataa attcattatg acgagaatac
    32161 tcgctgcgat aacccttttg ctgagtatcg tattgaccat tttggtcact attttttgtt
    32221 ccgtcccgat catcattgcc gggattgtaa aactattgct acctgtgcca gtcatttggc
    32281 gaaaggtttc gcgtttttgt gattttatga tgtattgctg gtgtgaaggt ctggcggtat
    32341 tactgcatct gaacccacac ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga
    32401 agaactggta tctgcttatt tgtaaccacc gtagttgggc agatattgtc gtactgtgcg
    32461 tgctgtttcg taagcacatt ccgatgaata aatatttcct caagcaacag ctggcctggg
    32521 tgccatttat tggcctggcg tgctgggcgc tggatatgcc gtttatgaag cgttattccc
    32581 gcgcttattt attacgtcat ccggagcgac gtggtaaaga tgttgaaacc actcggcgtt
    32641 cttgtgaaaa gtttcgcctg catcccacca ccattgtcaa tttcgttgaa ggctcccgtt
    32701 tcacgcaaga aaaacatcag caaacccact ccacttttca aaacttgttg ccaccaaagg
    32761 cggcaggcat tgcgatggcg ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg
    32821 ttacgctgtg ttatccggac aataaccgtc agccgttctt cgatatgtta agcggtaaac
    32881 tgacgcgaat tgtcgttcat gtggatttac agcctattgc cgatgagtta catggcgact
    32941 acatcaatga caaaagcttt aagcgccatt ttcagcaatg gctgaactca ctgtggcagg
    33001 aaaaagaccg acttctgacg tcgttgatgt catcacagcg gcaggacaag taaattttgc
    33061 tcgcctctga atgagcagag gcgagtgagt attttaatga acaaaacgtc cggcacgaga
    33121 cataaattct tcttcgctca tctcctgtcc gttaaaaaca actttacccg gtgtataacg
    33181 cagttgcagc gaggcggtat tgttgtcaat cgtgattaac tgaaacatct gacctaaggt
    33241 catcatcccg ctgatttgtt tatcagcccg cttttgcgct ttttccgcat ccattccttc
    33301 agataaatta agctgttttg cggtttctgt ggcgacatta agcggtaact tcacatcaaa
    33361 attgagcgat ttaatatctt tgtttgtgga tgatgaagat ttggctgggt cggcaatact
    33421 gatatccaga ttggcattta gttcgccgag tgcgttcttc cattttaccg gttgtttaat
    33481 ggtcggctca cttttttgta ataacggtaa atactctttg aacaatgcag cattcacttc
    33541 ttgcagagca acttcatcgt ttgctaactc agggtgtgca gcaagctgct tctgcattgc
    33601 aatgttatac tggatgataa attggcgcac cgctgaggga tcaatagatt cagcaatcaa
    33661 tgaccactca ccactaccga gttgctgatt cccttttgtt aacttatcaa gttcgtaggt
    33721 cagattgaca ttgatataat ctttgtcttt aacgcgatcc agcatggttt ttgcaacgaa
    33781 agcatcgatt tgggcaacgt cttccccgtg attgataatg ttaatctgat taaatttcga
    33841 ctcgctttca ccaaccggaa atttcttttc ttctaatcgc gccagagaat caatggtaaa
    33901 actttttgcc gttactttgt tcattggtga taattgcaga acaatatctt cgactttgcc
    33961 ttcaatatcc aggttagaca atgaactacc atcaccatta aaagtaaact ggccctcacc
    34021 aaaagaaaac tttccatact ctgcaggggc aacatctaat gtcgtagaga atgagccacc
    34081 aaacgcgaat cgcaatgtcg cttccacggg cgatttattt ttcgcggcga taaatagcgg
    34141 ttgtgtcagt tcattattca ctaagttcac ttttgccgct gccagtgctg ggatgacatt
    34201 accatgcatt aacattgtga tgggcaacgg accgtgctca acatccacat caaatacaac
    34261 tttctggcct ggcttgatat tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg
    34321 aaaacgagaa ttaaaaaaac ctttgtcaaa atttttataa cttaacgtca tatcgtaggc
    34381 atgttcacct ttctttttag catcgttaaa atctttaata aacttttcga caccaggctg
    34441 aatttgcgta cctgtgtacc atgtgccacc accccagatt acggctaacg caacaataac
    34501 acctgtagct gacttatgta tcatataaat attccctgat aaaacagcaa aactttaaat
    34561 atccgcttat gctgaaataa tgtccagcgg caggatgcat tatcagaatg tatttatatt
    34621 tattaaataa taaaaaaagc ccgtgaatat tcacgggttt tatgtaattt acatttaatt
    34681 attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa acatccatat
    34741 tgctggtatc catgccctgc ggattcagct gatatttacc gttaacaaac atcgccggaa
    34801 caccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga gatttcacca
    34861 cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg ataaatacat
    34921 cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca aacagcggaa
    34981 cagtcacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga gtcagatctt
    35041 tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg ccttccggca
    35101 gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag cagtgcgggc
    35161 agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt tccagggtag
    35221 tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa actaaaccag
    35281 ccagcggcag ccaaatcttt ttcatgatct actctctccg attaatacat aggtgttaat
    35341 tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt ttgtcgcagc
    35401 cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc agcccaacgc
    35461 cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg
    35521 atttcagcgg tgtcgaactc gctaaattct tcataagctt caataatagt ttccagttgc
    35581 atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat agctggacca
    35641 ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat attcccggcg
    35701 tggcagtctc catgtagccg cagaacggtg aagtcttccc gccagtgtgc tgtaacggcg
    35761 gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg catcagtgta
    35821 gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat ggtcgggcga
    35881 tggataaaaa gctgtttgcg ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct
    35941 tccatctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg
    36001 aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg tgctgcgacc
    36061 ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat ttgatcggct
    36121 gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgatcttc gtcctgaaat
    36181 tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac ccggatccca
    36241 tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg
    36301 ttattcatcc catcatccgg aaaacattac gaatgacgaa ggatatcatt tcacgatgat
    36361 tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc gtaacagcgc
    36421 cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat ctttcgcgga
    36481 atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca gttcgccgtc
    36541 aaaacctgac tctttcgcca gtttctgcaa aaattgcagc aggttaaggt ctggctcttt
    36601 ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt tcatagtgat
    36661 gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa agaggcgata
    36721 tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa agccagacga
    36781 atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg gcaacatgtc
    36841 gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgctaatcg tcatcaggaa
    36901 atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta cccaggccct
    36961 ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt gttttgcccg
    37021 tgcgatacgc cttacattcc ccctgattta gcagccaggc ttaatcatca gcgcaaagat
    37081 gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc tctggtaaac
    37141 cgcgctattg agcctttatt actggaatat ctgcaagcag gagaacgccg ggtaatggta
    37201 tttatgcgtc tggctggcgg tcatgcggtt gatttcagcg atcataaaga tgcatttgtt
    37261 aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata ccgttactcg
    37321 cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg atcccggcat
    37381 tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat atggatgttg
    37441 ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa accatcgttg
    37501 ccagccagca acgatgggcc ttgatgacgg aaacaccaga cgaagaagag ctggatctac
    37561 agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc gaagggttta
    37621 agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat cgacctgaag
    37681 aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt aatcttgatg
    37741 tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg gagtggatgc
    37801 aaaagcagaa cggataagca agagtaggct ggataagacg cgccagcgtc gcatccggca
    37861 tttttttctg cgccaa
//
LOCUS       DAFNBJ010000026        36446 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000026 DAFNBJ010000000
VERSION     DAFNBJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.026"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            317..1690
                     /gene="aroP"
                     /locus_tag="L2X49_001317"
     CDS             317..1690
                     /gene="aroP"
                     /locus_tag="L2X49_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBN4796429.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1733..2587)
                     /gene="ampE"
                     /locus_tag="L2X49_001318"
     CDS             complement(1733..2587)
                     /gene="ampE"
                     /locus_tag="L2X49_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBN4796430.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2584..3135)
                     /gene="ampD"
                     /locus_tag="L2X49_001319"
     CDS             complement(2584..3135)
                     /gene="ampD"
                     /locus_tag="L2X49_001319"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBN4796431.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3223..4116
                     /gene="nadC"
                     /locus_tag="L2X49_001320"
     CDS             3223..4116
                     /gene="nadC"
                     /locus_tag="L2X49_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBN4796432.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4319..4759
                     /gene="ppdD"
                     /locus_tag="L2X49_001321"
     CDS             4319..4759
                     /gene="ppdD"
                     /locus_tag="L2X49_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBN4796433.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4769..6154
                     /gene="gspE"
                     /locus_tag="L2X49_001322"
     CDS             4769..6154
                     /gene="gspE"
                     /locus_tag="L2X49_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HBN4796434.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLARTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6144..7346
                     /gene="hofC"
                     /locus_tag="L2X49_001323"
     CDS             6144..7346
                     /gene="hofC"
                     /locus_tag="L2X49_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBN4796435.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAILHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRENTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7381..8424)
                     /gene="guaC"
                     /locus_tag="L2X49_001324"
     CDS             complement(7381..8424)
                     /gene="guaC"
                     /locus_tag="L2X49_001324"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBN4796436.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGYEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8580..8624)
                     /locus_tag="L2X49_001325"
     CDS             complement(8580..8624)
                     /locus_tag="L2X49_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796437.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8649..9269
                     /gene="coaE"
                     /locus_tag="L2X49_001326"
     CDS             8649..9269
                     /gene="coaE"
                     /locus_tag="L2X49_001326"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBN4796438.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9269..10012
                     /gene="zapD"
                     /locus_tag="L2X49_001327"
     CDS             9269..10012
                     /gene="zapD"
                     /locus_tag="L2X49_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBN4796439.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            10022..10219
                     /gene="yacG"
                     /locus_tag="L2X49_001328"
     CDS             10022..10219
                     /gene="yacG"
                     /locus_tag="L2X49_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBN4796440.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10435..10824)
                     /gene="mutT"
                     /locus_tag="L2X49_001329"
     CDS             complement(10435..10824)
                     /gene="mutT"
                     /locus_tag="L2X49_001329"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBN4796441.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGKPWGKEGQPG
                     EWMALVDLNADDFPPANEPVIAKLKRL"
     gene            complement(10884..13589)
                     /gene="secA"
                     /locus_tag="L2X49_001330"
     CDS             complement(10884..13589)
                     /gene="secA"
                     /locus_tag="L2X49_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBN4796442.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13651..14163)
                     /gene="secM"
                     /locus_tag="L2X49_001331"
     CDS             complement(13651..14163)
                     /gene="secM"
                     /locus_tag="L2X49_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBN4796443.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14394..15311)
                     /gene="lpxC"
                     /locus_tag="L2X49_001332"
     CDS             complement(14394..15311)
                     /gene="lpxC"
                     /locus_tag="L2X49_001332"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBN4796444.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15412..16563)
                     /gene="ftsZ"
                     /locus_tag="L2X49_001333"
     CDS             complement(15412..16563)
                     /gene="ftsZ"
                     /locus_tag="L2X49_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBN4796445.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16624..17886)
                     /gene="ftsA"
                     /locus_tag="L2X49_001334"
     CDS             complement(16624..17886)
                     /gene="ftsA"
                     /locus_tag="L2X49_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBN4796446.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17883..18713)
                     /gene="ftsQ"
                     /locus_tag="L2X49_001335"
     CDS             complement(17883..18713)
                     /gene="ftsQ"
                     /locus_tag="L2X49_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBN4796447.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPERTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18715..19635)
                     /gene="ddlB"
                     /locus_tag="L2X49_001336"
     CDS             complement(18715..19635)
                     /gene="ddlB"
                     /locus_tag="L2X49_001336"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBN4796448.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19628..21103)
                     /gene="murC"
                     /locus_tag="L2X49_001337"
     CDS             complement(19628..21103)
                     /gene="murC"
                     /locus_tag="L2X49_001337"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBN4796449.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21157..22224)
                     /gene="murG"
                     /locus_tag="L2X49_001338"
     CDS             complement(21157..22224)
                     /gene="murG"
                     /locus_tag="L2X49_001338"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBN4796450.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22221..23465)
                     /gene="ftsW"
                     /locus_tag="L2X49_001339"
     CDS             complement(22221..23465)
                     /gene="ftsW"
                     /locus_tag="L2X49_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBN4796451.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23465..24781)
                     /gene="murD"
                     /locus_tag="L2X49_001340"
     CDS             complement(23465..24781)
                     /gene="murD"
                     /locus_tag="L2X49_001340"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBN4796452.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFAGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24784..25866)
                     /gene="mraY"
                     /locus_tag="L2X49_001341"
     CDS             complement(24784..25866)
                     /gene="mraY"
                     /locus_tag="L2X49_001341"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBN4796453.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25860..27218)
                     /gene="murF"
                     /locus_tag="L2X49_001342"
     CDS             complement(25860..27218)
                     /gene="murF"
                     /locus_tag="L2X49_001342"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBN4796454.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27215..28702)
                     /gene="murE"
                     /locus_tag="L2X49_001343"
     CDS             complement(27215..28702)
                     /gene="murE"
                     /locus_tag="L2X49_001343"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBN4796455.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTALGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28689..30467)
                     /gene="ftsI"
                     /locus_tag="L2X49_001344"
     CDS             complement(28689..30467)
                     /gene="ftsI"
                     /locus_tag="L2X49_001344"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBN4796456.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINYRINGHEIKDVARYSKLTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKA
                     TNLGLVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSI
                     TKVDPPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPD
                     GRYINKYIAYTAGVAPASQPRFALVVVLNDPQAGKYYGGAVSAPVFGAIMGGVLRTMN
                     IEPDALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30483..30848)
                     /gene="ftsL"
                     /locus_tag="L2X49_001345"
     CDS             complement(30483..30848)
                     /gene="ftsL"
                     /locus_tag="L2X49_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBN4796457.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30845..31786)
                     /gene="rsmH"
                     /locus_tag="L2X49_001346"
     CDS             complement(30845..31786)
                     /gene="rsmH"
                     /locus_tag="L2X49_001346"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBN4796458.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31788..32246)
                     /gene="mraZ"
                     /locus_tag="L2X49_001347"
     CDS             complement(31788..32246)
                     /gene="mraZ"
                     /locus_tag="L2X49_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBN4796459.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32848..33852)
                     /gene="cra"
                     /locus_tag="L2X49_001348"
     CDS             complement(32848..33852)
                     /gene="cra"
                     /locus_tag="L2X49_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBN4796460.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33934..34020)
                     /locus_tag="L2X49_001349"
     CDS             complement(33934..34020)
                     /locus_tag="L2X49_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBN4796461.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(34032..34523)
                     /gene="ilvN"
                     /locus_tag="L2X49_001350"
     CDS             complement(34032..34523)
                     /gene="ilvN"
                     /locus_tag="L2X49_001350"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBN4796462.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34526..36250)
                     /gene="ilvI"
                     /locus_tag="L2X49_001351"
     CDS             complement(34526..36250)
                     /gene="ilvI"
                     /locus_tag="L2X49_001351"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBN4796463.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36369..36413
                     /locus_tag="L2X49_001352"
     CDS             36369..36413
                     /locus_tag="L2X49_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796464.1"
                     /translation="MKRNDKILHYEGLN"
BASE COUNT         8714 a        10209 c         8949 g         8574 t
ORIGIN      
        1 taaaaatccg aattgaaccg attcacttac caattttgtg atttttaatt caattaaaac
       61 gaatttaaat tcattctaca tattgagagg ggttgaggct gagctttaca aacggtttct
      121 ttttaagcaa ctcatcttca accatgcata aagcgggtgc attcgctgcc gcataccatt
      181 attcttgatc tgacggaagt ctttttgtaa caattcaaac ttctttgatg taaacaaatt
      241 aatacaacaa acggaattgc aaacttacac acgcatcact gcgtagatca aaaaaacaac
      301 caccgcacga ggtttcatga tggaaggtca acagcacggc gagcagctaa agcgcggcct
      361 taaaaaccgc catattcagc ttatcgcgct gggtggcgcg atagggaccg ggttattcct
      421 gggtagcgcc tccgtaatac agtccgcagg gccagggatt atcctgggtt acgccattgc
      481 tggttttatc gcctttctga tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt
      541 cgcaggctcc tttagccact ttgcttataa atactggggc agttttgccg gtttcgcctc
      601 tggctggaac tactgggtac tgtacgtttt agttgccatg gctgagctga ctgccgtggg
      661 taaatacatt cagttctggt atccggaaat ccccacctgg gtttctgccg ccgtattctt
      721 tgtggtgatt aacgccatca acctgaccaa cgttaaagtg tttggcgaga tggagttctg
      781 gtttgccatt atcaaagtta tcgcggtggt agcgatgatc atcttcggcg gctggctgct
      841 attcagtggc aacggcggcc cgcaggcgac cgttagcaac ctgtgggatc agggtggttt
      901 cctgccgcac ggcttcaccg ggctggtgat gatgatggcg attatcatgt tctcgttcgg
      961 tggtctggaa ctggtgggga tcaccgcagc agaagctgat aacccggagc aaagtatacc
     1021 gaaagcaact aaccaggtta tctaccgcat cctgattttc tatattggtt cgttagccgt
     1081 tctgctctca ctgatgccgt ggacccgcgt taccgccgat accagtccgt ttgtgctgat
     1141 cttccacgag ttaggcgata cctttgtggc gaatgcgctg aacatcgtgg tactgactgc
     1201 ggcgctctcc gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc
     1261 acaacagggt aatgcgccaa aagcgctggc gtctgtcgat aaacgtggtg taccagtaaa
     1321 taccattctg gtgtctgcac tggtaacggc gttgtgcgta ctgattaact accttgcccc
     1381 agagtccgct ttcggactgt taatggcgct ggtggtatct gcactggtaa tcaactgggc
     1441 gatgattagc ctggcgcata tgaaattccg tcgcgccaag caggaacaag gcgtggtaac
     1501 tcgcttccct gctctgcttt atccgctggg taactggatc tgcctgctgt ttatggcggc
     1561 ggtactggtg attatgctga tgaccccagg aatggcgatt tcggtatacc tgatcccggt
     1621 atggctgatc gtgttaggta tcggctatct gtttaaagag aaaaccgcca aagccgtaaa
     1681 agcgcattaa tctctctacg ccctcacccg tacagggtga gggcaataat ctttacacca
     1741 acgccccgta aatcgtcagt agtgcaatca ccaccacgac cacgaacgag gttttcttcg
     1801 ccattgaaac cgctgccttc ggcgtctcca ccttatcgac atgcggttca cgcgccagag
     1861 agaactgcgc cagacgcgtt aacacctgat actgcgaagt atggaaatca cccagcgaag
     1921 caaaccaggc cggtaacgct ttctcaccat gaccgatcaa ggcatatacc acacccgcaa
     1981 gacgaaccgg cacccaatcc agtacatgaa gcacggcatc aatgccggac tgtaaacgat
     2041 gatgcggcgt ctgatatcgc gccagccagt attgccatgc acgcaaaaac gcatacccca
     2101 tcagcgtaac gggtccccag gttcccccca caatcagcca gaacagcggt gcaagataaa
     2161 aacgaaagtt aatccacagc aatgcatttt gcagctcacg caaatactca cgttcgtcgc
     2221 agcctgccgg gacgccgtga atcatggtga gttcgccagc catcgtggca cgggcatggc
     2281 tatcattacg tgaagcagct gtcagataag catgataatg aagacgaact ttacctgcgc
     2341 caatacacag caaaccaatc agcagccaca ccagtagcgt gggaacgttg aacaatactc
     2401 cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc aataatggtc atgcctaacg
     2461 tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct
     2521 gccagtgctc gcccaactta aacaggcgct cgaaaattaa caccagtaag gttgtaaata
     2581 gcgtcatgtt gtctccttgc tgaccagcac acgaaaccgt gcccaatcaa atgcaggacc
     2641 gggatcggtt ttccgatccg gcgcaatatc acaatggccc gtcatgtttt tagcgatatc
     2701 cggatagcaa tcaatcagtg cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt
     2761 atacgccagc gtatcggtgc cttcaagctc aatcccaata gaaaaatcat tgcagcgttc
     2821 gcgcccctga tactgagaga ctcccgcatg ccatgcacgt ttatcgaaag gaacatactg
     2881 gactatttca ccatcacggc gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc
     2941 agcaaagaaa ggatgtgcct gcggatcaat agttccagtg aataatgcgt cgatccacgg
     3001 accgccaaac tcgcctggcg gcaggctaat attgtgcacc accagcaggg tgggtgtttc
     3061 gtcatccggg cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc
     3121 ctgttctaac aacatgcagg atctccttat atgtggtgct aatacccggt tcagagtagc
     3181 atgtttctac cttatgattc gttagctatc tggagtttta acatgccgcc tcgccgctat
     3241 aaccctgaca cccgacgtga cgagctgctg gaacgcatta atctcgatat ccccggcgcg
     3301 gtggcccagg cgctgcggga agatttaggc ggaacagtcg atgccaacaa tgatattacg
     3361 gcaaaacttt taccggaaaa ttctcgctct catgccacgg tgatcacccg cgagaatggc
     3421 gtcttttgcg gcaaacgctg ggttgaagag gtgtttattc aactggcagg cgacgatgtc
     3481 accataatct ggcatgtgga tgacggcgat gtcatcaatg ccaatcaatc cttgttcgaa
     3541 cttgaaggcc catcccgcgt gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa
     3601 accctttcag gagttgccag taaggtacgc cactatgtcg aattgctgga aggcaccaac
     3661 acgcagttgt tggatacgcg caaaacctta cccggcctgc gttcagctct gaaatacgcg
     3721 gtactttgcg gcggcggagc gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa
     3781 gaaaaccata ttattgcctc cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg
     3841 cacccggatg cgccagtaga agtcgaagta gagaatctgg aagaacttga tgaagccctg
     3901 aaagcaggag ccgatatcat catgctggat aacttcgaaa cagaacagat gcgcgaagcc
     3961 gtcaaacgca ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca
     4021 ctgcgtgaat ttgccgaaac gggcgtggac tttatctccg tcggtgcgct aactaaacac
     4081 gtacaagcac tcgacctttc aatgcgtttt cgctaatagt tgacagattt atccgctcca
     4141 tcgcgggcgg ataatctccc cttccccaac tttcttcgta acgcctcgca aatttcttgt
     4201 ctttcgctgc aacgaaatag aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc
     4261 tcgcctgtgc tgatggcgtt tgccaaagta gcgccaacca aatcaaggag cgaaacagat
     4321 ggacaagcaa cgcggtttta cacttatcga actgatggtg gttattggca tcattgccat
     4381 tttaagcgcc attggtattc ccgcttatca aaactacctg cgcaaagccg cactcaccga
     4441 catgctacaa acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg
     4501 tggattagat acctgcgacg gtggcagcaa tggcattccc tcgcctacca ccacccacta
     4561 tgtttcagcc atgagtgtgg caaaaggcgt ggtgtcgctg accgggcaag aaagtctcaa
     4621 tggactaagc gtcgtcatga caccgggttg ggataacgca aacggcgtca ccggctggac
     4681 gcgcaactgc aatattcaaa gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg
     4741 ctttgatgac gccaactaag gagcggcaat gaatattcca cagctcacgg ccctgtgtct
     4801 gcgttatcag ggagtcttgc tggatgccag cgaagaagtg gttcatgttg cggtggtcga
     4861 tgccccctca catgagttgc tggacgcatt gcatttcgct accaccaaac gtattgagat
     4921 cacctgctgg acgcgccaac aaatggaagg tcacgccagt cgcacacaac agacattgcc
     4981 cgtagctgtt caggagaagc atcagcccaa agcagagttg ctagctcgaa cgttacaatc
     5041 tgcgctggaa caacgcgcgt ctgatattca tatcgaacca gcggacaatg cctaccgcat
     5101 ccgcttgcgt atcgacggcg tattgcatcc tttaccggat gtttcaccgg atgccggagt
     5161 cgcattaacc gccagattaa aagtgctggg aaacctggat attgcggaac atcgcctgcc
     5221 gcaggacggg caattcactg tcgaactggc aggaaacgcc gtctcatttc gtattgcgac
     5281 cttaccatgt cggggtggtg aaaaggtggt attaaggttg ttacagcagg tgggtcaggc
     5341 actggatgtc aacacgcttg gaatgcagcc gttacaactg gcggactttg ctcatgcctt
     5401 gcaacaacca cagggactgg tgctggtaac tggccctacc ggcagcggca aaacggtcac
     5461 gctttatagt gccctgcaaa cgctgaatac cgctgacatt aatatttgta gcgtcgaaga
     5521 tccggttgag atccccatag ccggactaaa ccagacgcaa atccatccgc gtgccgggct
     5581 cacctttcag ggcgttttgc gtgcgttatt gcgccaggat cctgacgtca tcatgatcgg
     5641 agagatccgc gatggcgaaa cagcagagat cgctattaaa gcggcgcaaa ctggtcacct
     5701 ggtgttgtct accctacaca ctaattccac ctgcgaaacg ctggtacgtt tacagcaaat
     5761 gggagtcgcc cgctggatgc tctcatcagc gcttacgctg gtaatagccc agcgtctggt
     5821 acgtaaactt tgcccacatt gtcgccagca gcaaggggag cccatccata ttccagtcaa
     5881 tgtatggccg tcgccgctgc cccactggca ggcacccggt tgtgtacatt gctaccacgg
     5941 tttttatggt cgtacggcct tatttgaagt tctgcccata acgccggtca ttcgtcagct
     6001 tatttccgct aataccgacg ttgaatcgct ggaaacgcac gcacgacagg cgggtatgtg
     6061 tacgcttttt gaaaacggct gcctggccgt ggagcaaggc ttaaccacct ttgaagagtt
     6121 aatccgcgta ctggggatgc cgcatggcga gtaagcaact ctggcgctgg catggcataa
     6181 ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga gagccgtgct ttgctgctca
     6241 tggcactaca gcaacagatg gttaccccac ttagcctgaa gcgaatcgcc atcaattctg
     6301 cgcagtggcg aggagataaa agcgcggaag tcattcatca actggcgacg ctactcaaag
     6361 ccgggttaac gctttctgaa gggctggcac tgctggcgga acagcatccc agtaagcaat
     6421 ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca gggcattgct ttttccaatg
     6481 ccttattacc ctggtcagag gtatttccgc cactctatca ggcgatgatc cgcacgggtg
     6541 aactgaccgg taagctggat gaatgctgct ttgaactggc gcgtcagcaa aaagcccagc
     6601 gtcagttgac cgacaaagtg aaatcagcgt tacgttatcc catcatcatt ttagcgatgg
     6661 caatcatggt ggttgtggca attctgcatt ttgttctgcc ggagtttgcc gctatctata
     6721 agaccttcaa caccccacta ccggcactaa cgcaggggat catgacgctg gcagacttta
     6781 gtggcgaatg gggctggctg ctggtgttgt tcggctttct gctggcgata gccaataagt
     6841 tgctgatgcg ccgccctacc tggcttatcg cgcggcagaa attgttgtta cgaatcccga
     6901 ttatgggttc actgatgagg ggacaaaaac tcacgcagat ctttacgatt ctggcgctga
     6961 cacaaagtgc aggcattact tttttacagg gcgtagagag cgtcagagaa acaatgcgct
     7021 gcccgtactg ggtgcaactt ctgacacaaa tccagcacga tatcagtaac ggtcatccca
     7081 tctggctggc gctaaaaaat gccggtgagt ttagcccgct ctgtttgcaa ttagtgagaa
     7141 caggagaggc atccggctct ctggatctca tgttagacaa cctcgcccat catcatcggg
     7201 aaaacacaat ggcgctggcg gataacctcg cagccttact ggaaccggcg ttgctgatca
     7261 taacgggagg aattattggt acgctggtgg tggcaatgta tctgccaatt ttccatttag
     7321 gcgatgcgat gagtgggatg ggataacgct ggcgtgttgc tccacgccag cgttgggaga
     7381 ttacaggttg ttgaagatgc ggttttcttg ttcctgcaca cgaataaacg tggtgcgctt
     7441 ggtcagctct ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca ggccgcccaa
     7501 aatatctcgc gcggtatttt caaccgggcc tcgcagcggc agcttaacgg ttttaccttc
     7561 tgctgcgcga tattccgcaa cgccgccaac gtgacgtttc atcgcagact cggagctcat
     7621 gccgtagaac agcataaatt tctcgccgtt ctcctcaacg atgcgaccgc cgctctcttc
     7681 gtagcccgcc agcatgccgc caagcatgac gaaatcggca ccgccgccaa aggctttcgc
     7741 cacatcgccc ggcgtggtgc agccaccatc gctgacgatc attccgccca gaccgtgcgc
     7801 agcatcggca cattcgatta ccgcagaaag ttgcggataa ccgacgcctg ttttgacgcg
     7861 agttgtacaa acagaacctg ggccaatgcc aactttaacg atatcggcac ctgagaggat
     7921 aagctcctca cacatttcac cagtcactac gttaccagca caaatggttt tggtcggcca
     7981 cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg gaataaccat tcgccacgtc
     8041 aatacaaacg aagtttaatg ccgggttcag gtcgagaatc tgtttagttt tttcgaaatc
     8101 cgcatcagac gtaccggtag aaaccatcac atgtttcagc acatcagcgg aagaattgtt
     8161 gataaacgct tgccactctt cgacagaata gtgtttatgc acagcagtca aaatatcaaa
     8221 agaagccagc gcagaggcca tagaaaatgt gcctacggtg tccatatttg cggcgataat
     8281 cggcacgccg gaccagctct gacctgaatg tttgaaggtg aattgacgtt ccagttcaac
     8341 atcggaacgg cttttaagag tggagcgttt agggcggatg agaacgtctt taaaacctaa
     8401 cttcagatct tcttcaatac gcatgtgcgg attcctgggg ttaatggcta taagggtaag
     8461 agcgcaactc cagtgacgct atcatacgca ctaatcaatg cggcgcaaga ctgcgaaatt
     8521 gtcttttttt acgctacaat cccataaatt tacctggtaa atagtgagcg aacgtttcat
     8581 caatttcgct ttcgtgcact catttttaac gttttgataa tcatgatgaa ttcccgggaa
     8641 tataaattat gaggtatata gttgccttaa cgggaggcat tggcagtggc aagagtaccg
     8701 ttgccaatgc gtttgctgat ctcggaatta acgtcattga tgccgatatt attgcgcgtc
     8761 aggtggttga accaggtgca cctgcgctac atgccattgc tgatcacttt ggcgctaaca
     8821 tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg cgagcggatc ttcgccaacc
     8881 cggaagagaa aaactggctt aacgccctgc tgcatccgct gattcagcaa gagacgcaac
     8941 accagatcca gcaagctact tccccctatg tactgtgggt tgtgccattg ctggtagaaa
     9001 actcactgta taaaaaagcg aatcgagtgc tggtggtgga tgtcagccca gaaacgcaac
     9061 ttaagcgcac catgcagcgc gatgatgtaa ctcgcgagca tgtcgaacaa atccttgctg
     9121 ctcaggcaac gcgcgaagcc cgccttgccg tggcagatga cgtcattgat aataacggcg
     9181 caccggatgc tatcgcatcg gatgttgccc gcctgcacgc acactatttg cagcttgcgt
     9241 cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag gtcctttttg aacatccact
     9301 aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg attcagcaac tcaccgttaa
     9361 tttacccatc gttgaccacg ctggcgcgct gcatttcttc cgtaatgtca gtgaattact
     9421 ggatgttttc gagcgcggcg aagtccgcac tgagctgttg aaagaacttg accggcagca
     9481 acgtaaactc cagacctgga ttggcgtgcc tggcgtggac cagagccgta ttgaagcatt
     9541 aattcagcag ttaaaagcgg cggggagcgt attaatttcc gcgccgcgta tcgggcaatt
     9601 tctgcgtgaa gatcgtttga ttgctctggt gcgtcagcga ctgagcatcc caggcggctg
     9661 ttgcagcttt gatttaccta cattgcacat ttggctgcat ctaccccagg cgcagcgcga
     9721 cagccaggta gaaacctgga ttgccagcct gaacccgctc acccaggcac ttaccatggt
     9781 gctggattta attcgccagt cggccccctt ccgtaaacaa accagcctga atggttttta
     9841 tcaggataac ggtggcgatg ccgacttgct gcgcctgaat ctgtcgctcg attcacagct
     9901 ttatccgcaa atttccggac ataagagccg ttttgccatt cgttttatgc cgctggacac
     9961 tgaaaacgga caggtaccgg aacgtctgga tttcgaactg gcctgttgct aaggagtaaa
    10021 aatgtcagaa actattacgg tgaattgccc aacctgcggg aaaacggtgg tgtggggtga
    10081 aatcagcccg tttcggccat tttgctccaa acgttgtcag ctgatcgacc tcggagaatg
    10141 ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt tccgaaagcg atgactggag
    10201 cgaagagcca aagcagtgac atttgtctga tgccgcacgt aggcctgata agacgcggac
    10261 agcgtcgcat caggcatctt gtgcaaatgt cggatgcggc gtgaacgcct tatccgacct
    10321 acaaaaacaa atttatcaat gagttagaaa tccctgtagg cctgataaga cgcgccagcg
    10381 tcgcatcagg catcttgtgc gaatgtcgga tgcggcgaaa acgccttatc tgacctacag
    10441 acgtttaagc ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg cattaagatc
    10501 gaccagcgcc atccactcac cgggttgccc ttctttaccc cacggcttcc cttcccagcg
    10561 ttcgaccagc cagaaccaca gtgttatatg cctgtccggg aattcatatt ccagtttttc
    10621 aaatagcgaa aaatgttgtg gggtaatccc gacttcttcc tgaagttcac gcaccaccgc
    10681 ctgttccggc gtttcaccca tttcaatttt accgccggga aactccagtt tattcgccat
    10741 gtgcgcatct gctgcgcgac gcgttataaa gatttcattg ttctcgttgc gaataatacc
    10801 taccgcaatt tgcagctttt tcattgtctt aaacctataa aaaaggcgca gaatcctgcg
    10861 ccttttactt caactgttag cttttattgc aggcgaccat ggcactgctt gtatttttta
    10921 ccagaaccgc acgggcaagg atcgttacgt cctactttgc gttcaccggt ttgcgccgcc
    10981 agtgcagctg cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat ttgcgctaaa
    11041 cgctcggctt ccatacgacg ctgttgttcc agctcctcaa cttcttcagg catacgtacc
    11101 tgaactttgc tcagcgtact gataacttca tatttcaacg actccagcat cgctgcaaac
    11161 atggagaacg attcacgttt gtattcctgc ttcggatctt tctgtgcata gcctcgcagg
    11221 tggataccct gacgcagata gtccatcgct gccaggtgct ctttccacag ggagtcaagc
    11281 gtttgcagca tgacgccttt ctcgaagtga cgcatcatct ctgcaccaac cacttcttct
    11341 ttacgctgat acacttcgat ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc
    11401 agttcgggtt ctttatccag ccactcagca attggtaaat cgaggtcgaa atcgttcttc
    11461 agacgttcct gcagccccgg aatatcccac atttcttcca gcgactgcgg cggaatgtag
    11521 gcatcaatgg tggctttgaa cacatcttca cgaatgctgt tgatggtttc gctcacatcg
    11581 ctaacatcca gcagttcgtt acgctgggag taaatggcgc gacgctgatc gttagccacg
    11641 tcatcatatt ccagcagttg cttacgaatg tcgaagttac gactttcaac tttacgctgg
    11701 gcgttggcaa tcgctttggt cacccacggg tgttcaatgg cttcgcctgg cttcataccc
    11761 agtttacgca tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc
    11821 atcgacaggt agaaacggga agaaccagca tccccctgac gaccagaacg accgcgcaac
    11881 tggttatcga tacgacggga ttcgtgacgc tcggtaccga tgatatgcag gccacctgct
    11941 gccagtaccg catcgtgacg tacctgccag tcggctttaa ttttttcaat ttgctctgcg
    12001 gtcggatttt ccagcgcggc aacttctgcc tgccagctac caccgagcac aatatctgta
    12061 ccacgacccg ccatgttggt cgcgatagtc accgcagccg gataacctgc ctgagcaaca
    12121 atcgccgctt cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat accggctttg
    12181 gtcagttcgt ttgacaccag ctccgatttt tcgatggaga tagtacccac cagcaccggc
    12241 tggcctttcg cagtacgttc tttgatatct tcaatgatcg cctgaatttt ttccgcttca
    12301 gtcatgtaga ccaggtccgg caaatcttta cgaatcattg gacggttggt cggaacaacg
    12361 acggtatcca gcttgtagat ggagctaaat tcgaaagctt cggtatcagc agtaccggtc
    12421 atccccgcca gtttttcata cagacggaag tagttctgga aggtgatcga agccagcgtt
    12481 tggttttcgt tctggatctg cacaccttct ttcgcttcca cagcctggtg cagaccatcg
    12541 gaccagcgac ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat aacttcacca
    12601 tctttaacga tgtagtcgac gtcgcgggta aacagcgcat gagcgcgcag cgccgccgtt
    12661 acgtggtgca tcagcatgat gttggccgga gagtacagag actccccttc atccatgatg
    12721 ccctctttca ccagcagttc ttcaatcagc accagaccac gttcggtcag gttcacctgg
    12781 cgagattttt cgtccaccga gaagtggcct tcgccctgga aggtttcgga gtcttctttt
    12841 tcctgacgga tcaggtgcgg aataatttta ttcacgcgtt tatacatttc cgagctgtct
    12901 tctgccgggc cggaaatgat cagcggtgta cgcgcttcat cgatcaggat ggagtccact
    12961 tcgtccacca gcgcatagtg cagtttacgc tgtacacgtt cttcagggct gaacgccata
    13021 ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg
    13081 taagcttcgc gctttgccgg tgctggcatg cccggcaggt tgataccgac agtcaggcca
    13141 aggaattcaa acagcggacg gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg
    13201 gtaactacgt gcacgccttt accggttagt gcgttcaggt aagcaggcag cgttgcggtc
    13261 agggttttac cttcaccggt acgcatttcg gcgatgcagc gttcgttaag aaccataccg
    13321 ccgagtaact gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc ctcacgtacc
    13381 acggcgaaag cttccgggat cagattttcc agcacttcgc ctttttccag acgtgcacga
    13441 aactctgcgg ttttcccttt cagttcttcg tcggagagtt tttccatctc cggttccatg
    13501 gcattgatga tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg
    13561 aaaactttag ttaacaattt gattagcata ataaaatctc aaacgccccg cgttgcggag
    13621 ttaataaaat gaagtaaagg tttattgttg ttaggtgagg cgttgagggc cagcacggat
    13681 gccttgcgcc tggcttatcc agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc
    13741 ataatcaatg cgataaccct tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag
    13801 cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa gattcttcag caacgggcag
    13861 tgtttgcggt gccattgcga aagaaagatg acggattacc gtgcgaatgg catgttgatg
    13921 ccagtaatca acggaatagt tcgaattcgg gcggcgtgtg ttcgcttcca gcaaggccaa
    13981 ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc
    14041 gtttggttcg gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcaa ccatccctaa
    14101 taagagatgc ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca gtattccact
    14161 cacgttattg ccatcccgat cgtattcaaa cgtatttagc gcacaaatct tatcattaaa
    14221 gcctgcagtc cacagcacga atgaagataa gttgtgcgga aaagtgctta agaaaacagc
    14281 gttcgcaccg ttttttatag ctcttataaa attcaaaaaa aaacgactgg ttcacctggc
    14341 cggagagagt gccagatttg ccagtcgaat tttatacgac agtataaatg tcgttatgcc
    14401 agtacagctg aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca
    14461 tattcccagg cttcctgttt cgccaggaca gcctgcagca gtttgttatt cagtgcatga
    14521 ccggatttat aagcggtaaa tgcaccaata atattgtgac cacacatgaa caagtcaccg
    14581 atcgcatcga gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc
    14641 agtacgcgat aatcgtcaac aacgatggca caatcgaagc tgccgcccag gcacaaacca
    14701 cgggactgca gatattcgat atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg
    14761 cgcataaacg catcagcgga gaagttcatc gcatagcgct ggttgctgga atcaatagcc
    14821 ggatggttaa aatcgatggt gaaatccagc gaaaaaccat tgtacggctt aaattcagcc
    14881 cacttatcgc catcttcgac acgaacagtc tctttgatgc gaacaaattt tttggcgcag
    14941 ttcaactcgt cgataccggc gtcaagcagc aggtatacaa acggagcggc gctgccgtcc
    15001 atgatcggga tttccggcgc gttaacttcg ataacaatgt tatcgatgcc caggcccgcg
    15061 agagcagcat tgaggtgctc tacggttgaa atccgtacat catgctcgtt gaccagacac
    15121 gtacagagca tggtatcacg cacagatttg gcatcggccg ggaaatctac cggtggattc
    15181 aagtcggtgc gacgatagat gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg
    15241 actttcttgc cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt
    15301 tgtttgatca tcgtattatc tcgccaaatt acctatccaa ccgaagtgta ctatacattc
    15361 ggcgggccag tttagcacaa agagcctcga aacccaaatt ccagtcaatt cttaatcagc
    15421 ttgcttacgc aggaatgctg ggatatccag ataatccggc tctttcgcag tttgcggcgc
    15481 attgtcattc acgactttag caactggctt ctgctcctgg gtcagcggag ccatcccatg
    15541 ctgctggtag cgatccatca ctggctgctg aacctgctta ttggtcacca gagtgatttc
    15601 aggacgtttg tccatgccga tacctgtcgc aacaacagtt acgcgcagct cgtcattcat
    15661 atccgggtca agagaagtac cgataaccac agtcgcgttg tcggaagcaa atgcacggat
    15721 ggtgttacct accgtttcga actcatccag acgcaggtcg aagcccgctg tgatgttaac
    15781 cagcacgccg cgtgcgccag ataggtcgat atcttccagc agcggagaag aaatagccat
    15841 ttcagcagct tcttccgcac ggtcttcacc gctcgccacg ccagaaccca tcattgcgta
    15901 gcccatttca gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg
    15961 agtaatcagt tcggcgatgc cctgaacagc gcctttcagt acatcgttcg ctgcgccaaa
    16021 cgcatccagc agggagatac cgcggcccag aactttcagc agtttgtcgt tcgggatagt
    16081 gatcagagag tccacatgct tggacagttc agtgatcccc tgctccgcga atgccatacg
    16141 cttcttgcct tcaaagttga aaggcttagt gacgacagca acggtcagga tacccaaatc
    16201 ttttgccact tcagcgacga ctggtgctgc acctgtaccg gtaccaccac ccatacccgc
    16261 agcaataaag accatgtctg caccttccag cgccgcacgc agtgcatcgc gatcctcatc
    16321 agccgcattg cggccaactt ctggattagc gccagcgccc agtcctttgg tgataccgct
    16381 accgatttga atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat cggtatttac
    16441 cgcgaagaat tcaacacctt caatacgctc gcgcaccatg tgttcaacag cattaccgcc
    16501 gccgccgccg acgccgatga ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa
    16561 catagtttct ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa
    16621 aaattaaaac tcttttcgca gccaactatt gagtcgcttg atccacgagc caactgatgc
    16681 tgtaacacgt ttttctactt cagcttcacc gttaagatgt gactctttcc catagtgaag
    16741 caatcccacc gccgtcgaat aatacggctc ctgagcataa tccgttaaac cggtaatgtt
    16801 cagcggcgcg ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc
    16861 ttcgatctgc gctgcgccac cggttaatac aatgcctgcc gccaggtgat gtttaacccc
    16921 ttgttggcga agcttttcct gcaactgcaa tatctcttcg ttgaccaggt tgagcagctc
    16981 ggtatagcgc ggctcgatca cctctgccag ggtctgacgt tgcagactcc gcggcggacg
    17041 accacctacg ctcggcactt ccacgctctc atcttttcca acgatggaac ccagcgcaca
    17101 accgtggcga actttaatcg cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat
    17161 atcactggtc acgacattgc cagcataagg aattacctta gtgtggcgca atgccccacc
    17221 ggtataaacg gcgatatcca ttgtaccacc accgatatcg acgacgcaga cacccagttc
    17281 acgttcatct tccgtcaata ccgaataact tgatgccagt ccggcaaata tcagttggtc
    17341 aactttcagc ccacaacgtt caaccgcttt gacgatgttt ttcgccatat cgttgtgaca
    17401 tgtgatcagg tgcacttttg cctgcatccg cacgcccgaa agtcctaccg gattcttgat
    17461 cccttcctga tagtcaatcg catactcttg cgggatcaca tgcagcacac gatgctcatc
    17521 gcgcacacgc accgatttcg cggtatggac gacgttttcc acatcttctt gcgtcacttc
    17581 ttcttcagaa ataggcacca taccaatttc attctggcag ctgatgtgct taccagaaag
    17641 cgccagatat accgaagaga tctgacaatc tgccatcaat tctgcctggt caatggcgcg
    17701 ttgtacgcac ttgaccacgg attcgaggtc gttcaccccg cctttatcca taccacgcga
    17761 cgggcagctg cccacgccaa tgatattgac cataccgtcg ggcagaactt cccctactaa
    17821 agcggcaacc ttcgcggtac caatctccag tcctactacc agttttctgt ccgtcgcctt
    17881 gatcattgtt gttctgcctg tgcctgattt tgttgctgag tagattcctc tggcggcaag
    17941 ggcgcccagc ctactgccgc tccagagtca taacgcaaat caacgtagct aatccgtttg
    18001 ccatcggttt gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg agccaaacgt
    18061 ttcatcgtat cgccccggcc aagattgagc ttaatatcgt tattcagcgt caactgccag
    18121 gaacgccgcg cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc cagcatctgc
    18181 cccatttcgc gatagccctg caacacttca ttggcgctgc cttccgggcc atacagcatt
    18241 ggaagcacct gcttgctggt gcgttctggc ggcacgctga aggtatttcc ttccgcgtct
    18301 accatatgtt gatcattcca ccgcgcaatc ggcacatatt caaccagatg aatcttcaat
    18361 tcatcaggcc actgctttct gacgctcacc tgcttaatcc acggcaggcg ttgttctatt
    18421 tgcgtctgga tgatgttgac atcctgggtc ataaaggtac ccggctcacc caatgccagg
    18481 atcgactgcc ggatatcgtc attacgtgtg taatggcgtt caccggtcaa caccagcttt
    18541 gagagcggca ggcgttgcgc atcttccatc cagcccaaca cgacccagcc gctcaccaac
    18601 actgtcgtta aaacggtcag caggaaaagg atccccgcca gacgcgttcc attattgcgg
    18661 cgagaagaaa cctcttcttc gctgtttcgc gtgttcagag cagcctgcga catattagtc
    18721 cgccagttcc agaattcgta ctaccaactg cgagaagctc atacctgcct gacgtgccgc
    18781 catcggcacc aggctgtggc tggtcatacc cggtgaggta ttggcttcca gcagataaaa
    18841 ctgtccatcg ctgtccagca taacgtcaat acgtccccat cctttgcaac ctaacgtcgt
    18901 ccatgctttc agcactaatg cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg
    18961 gcagaaatac tgtgtctcat cagagagata cttcgcctca taatcataga aggttccgga
    19021 cggttgaata cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg
    19081 cccacttagc catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc
    19141 atcttgtaga gcattttctg ctactacttt tgacattccc acactggaac cttcgcggct
    19201 cggcttaacg ataaccggca aacccagagc agaaatttct gctaactgct tatcgctcag
    19261 gcctttttca aactctgcgc gggttaacgc tacccacggc gcgaccggta aaccggcacc
    19321 ttgccataga agtttgctgc gtagtttatc cattgaaagc gcagatgcca tcactccgct
    19381 tccggtataa ggcaagccca tcagctcgag catcccctgc agcgtaccat cttcaccgcc
    19441 gcgaccgtgt agcgcgataa acactttctg aaagcccatc gacttcagtt gcgtcacgtc
    19501 gacttctttc gggtcgacag gatacgcgtc aataccgcct tcacgcagtc cggctaacac
    19561 cgctgcgcca gaattcagag aaacttcccg ctcagcggag gtcccaccca acaggaccgc
    19621 gattttatca gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg atttcagcta
    19681 aagaacgagc aatttttcca atattaccag ccccctgaac gagaatcagg tcgttaccgg
    19741 ttaataccgg tgccagcatc tcggctaccc gcgccggatc cggcaccaga atgggatcaa
    19801 ttttcccacg tccacgaatt gtgcgacaca gcgaacggct gtccgctccc ggaattggcg
    19861 cttcgccagc cggatacact tccagcatca acagggtatc aacctgcgtc agcacattgg
    19921 cgaaatcatc atacaggtcg cgcgtacggg taaaacggtg cggctgaaac agcattacca
    19981 ggtttttatc cggccagcct gcgcgcgccg ctttaatggt ggcgtccact tcagtcgggt
    20041 ggtggccgta gtcatcgacc agcattgccg taccgctttt accattcact ggctccagcg
    20101 ggaattcacc gaggaaatca aaacggcgac cagtcccctg gaagctttca agcgcccgca
    20161 aaatagcctc gtcgtcaatg ccctcttccg tagcaaccgc aaccgcagct gcggcgttca
    20221 gcgcgttatg acgacctggc gcattcaggg tgacgcgcat cggctctttg tcctggcgca
    20281 gcagcgtaaa gtgcccctgc gggccaatct gctgataatc ttctacacgc acgtcggcat
    20341 cttcgctgaa gccgtaagtc gtggtctgac gccccactcg cggtaacaat tcgcggatca
    20401 ccggatcatc aacacacatc accgcacgac cgtaaaacgg caggttgtgc agaaaattaa
    20461 taaaagtctg ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga
    20521 tattggtgac aatcgccacc atcggttgca gatgcaggaa cgatgcatca ctctcatctg
    20581 cttcggcaat caggtaccga ccatgcccca aacgcgcatg aacccccgcc gcttttacca
    20641 gcccgccgtt aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg
    20701 cggtagttgt cgttttgccg tgcgttcctg caatggcgat gccatgacga aaacgcatta
    20761 actcagccag catttcggca cgacggatca ccggaatacg cgcttcatga gctgcgacaa
    20821 tttccgggtt gtcggcagaa atcgcgctgg aaacaacgac cacgctggca tcacgtacgt
    20881 tttccgggcg atggttgaaa taaatcgtcg ctcccagatt cattaactgc tgcgtgaccg
    20941 gatttggcgc taaatcggaa ccactgatct gataaccttc attggccaga acttcggcaa
    21001 taccgcccat accggcacca ccgatgccga caaaatgtat gtgccgaacg cgacgcattt
    21061 cgggcacgat ggaacgcagt tttgccaatt gttgtgtatt cattctttac gccattaact
    21121 tcttaaattc atacgatgca aaaggcatcg ctacaattac gcccgggcag cccggctcac
    21181 ttcatttgcc actcgctcgg tggcatccgg aatggatgca gcgcgggcgc gttctgccat
    21241 ggttaataag gtttctcgcg accacccggc cagggtgttg gcgacagcat ccacgctaag
    21301 ctgtggctgt tcgataattt tggctgcgcc cgctttttcc agcggtagcg cattccagta
    21361 ttgctggcgg tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat
    21421 ttcactcacc gttaaagcac cggagcggca aacgacgaca tccgcccacg catacgccgc
    21481 cgccatatca tcaataaatt ccgtcacttt atgctgcggt tgccccgctt cggcatacgc
    21541 ctgttcaacg gattgttgcg aacctttacc gctctgatgc cagatagtga ctgaatcacc
    21601 cagtttcgca gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct gggaaccacc
    21661 cactaccagc acacgaaccg gaccttcacg tccagccaaa cgttgctgcg gcaacggcag
    21721 cgccagtaca tcggtacgca ccgggttacc caccacttcc gcattaggga aagcgcctgg
    21781 aaacgcctgc atcactttgg tggcaatctt cgccagccat ttattggtta agcccgcaat
    21841 accgttttgt tcatgaagta caaccggaat gcctaacgac cacgcggcca gaccacctgg
    21901 acctgataca tagccgccca taccgagtac cacgtcaggt ttgtacgctt tcataatcgc
    21961 ccgcgcctga cgccaggcgt tgaagatacg cagcggcgca gctatcagtg cttttatacc
    22021 ttttccacgc agaccagaga tacgaatgaa atcaatttcg atgccatgtt ttggcactaa
    22081 gtccgcttcc atacggtcgg cagtccccag ccagcgaact tgccaaccct gagccattag
    22141 atggtgcgca accgccagtc ccgggaatac atgtccaccg gttccgcctg ccatcaccat
    22201 taatcgcttt ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga
    22261 cgcgtttcat aatcaatacg caacagcatc atgatggctg tcgacataat cagtaagctc
    22321 gaaccaccgt aactgatcag cggcaatgtc agacctttgg tcggtaacat ccccgccgcc
    22381 gcgcctacgt taaccagcgc ctggaagcta aaccagatgc caatagaaca ggcgagaaaa
    22441 ccggaaaaac ggtggtcaat ttctaacgct ttgcggccaa tcgacatcgc gcgaaaagcg
    22501 acgaagaata ccattaaaag tgccagcacc acaccgacat accccagttc ttcgccgata
    22561 atggcgaaaa taaagtcagt gtgcgcttcc ggcagatact ccagtttttg taccgagtta
    22621 cctaaacctt gtccccaaag ttcgccgcga ccaaacgcca tcagcgattg cgttaactga
    22681 tagccgctgc caaagggatc ttcccacggg ttccagaatg cggtaacacg gcggatacgg
    22741 tacggttcgg cgagtatcag caacacaacc gctgaaatgc ccataccgat aatggcaatg
    22801 aactgccaca atttcgctcc cgccaggaac aacatcgcca gcgtagtcac aaacaacacc
    22861 accaccgtac caaggtctgg ctgtgccagc agtaacactg ccaacaccag aatcacgccc
    22921 atcggtttca ggaagccgcg caggttatta cgtacttcgt cgcctttacg caccagatag
    22981 ttggcgatat aacaaaacag cgacagtttt gtcagctccg caggctggat acgcagcaaa
    23041 ccgagatcga tccaacgcga tgccccttta accgagctac ccactaccag cacgatcatc
    23101 agcaggatga tagatccgag cagcatcgtg gcactgtagc gttgccagaa ctccatcggc
    23161 agacgcagcg taatgatcgc cagaatgaat gccaggatca gatagacgcc atcacgcttc
    23221 gcgaagaaga acggatcgtt ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc
    23281 atgataaagc caatcgccgc aaggccgaag gtcagccaca gtaaggtgcg atcgtacatg
    23341 atcaggctgt cggtatcttt ttcccgcgag cccatcaccc agccttttag cgccgtggag
    23401 atccagacca ggatactgaa tcctggcagg cgcggcattt tcaggcgagg gagagataaa
    23461 cgcatcaacc taactccttc gccagacggg caaactcatt gcctcgttgt tcaaagttct
    23521 tgaactgatc aaggctggca caggccgggg agagcagaac catatcgccc ggctgaacac
    23581 gcggagccag caagcgcatc gcctgttcca tagtttcggt ttgttctgcc acttccgggc
    23641 gtagcgccgc cagctgcgca ccgtcacgac caaaacagta caaacgcacg ttgtcgccat
    23701 tcagatagcg cgccagtgga ctgaagtccg ccgatttacc atcgccgccc agcaacaaat
    23761 gcagcgtgcc gtctacctgc aagccattca acgccgcttc cgtactgccg acgttagtgg
    23821 ctttcgaatc gttaatccag cgtacgccat tgtgctccag caccacttca aagcgatgcg
    23881 gcagaccggc gaaggtggtt aacgctttca ggctgctggc gcgcggcaaa ccagctgcgt
    23941 ctgccagtgc cagtgctgcc agcgcattgg tgtagttatg ctgcccggaa agtttcatct
    24001 ctttcacgtt cagcactttc tcgcctttca ctcgcagcca ggtttctccc tgctgatgat
    24061 tcaggtgata gtcacccatg ttgacgccaa agctgacgca gcgttcatca gcaccgcgaa
    24121 tcggcattgt taaggcatca tcagcattga cgacacaaac tttcgcattt tcgtaaatac
    24181 gcaattttgc cgcacggtat tgctgcaaac caaacggata gcggtccata tgatcttcag
    24241 tcacgttcag aatggtcgcc gccaccgcct gtaagctgga agtggtttcc agctggaagc
    24301 tcgacagttc cagcacgtac agttcgcact cagcatcaag cagcatcaac gctggcaggc
    24361 caatattgcc acccacacca acgttaaccc ccgccgcttt cgccatttca cccactagcg
    24421 tggtgaccgt gcttttgccg ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc
    24481 gacagaacag ctcgatatcg ccaacgattt cgattccggc atcagcggca gcgcttaagg
    24541 atggatgcgc cagtgcaata ccgggactgg cgacaatcag atctgccgcc atcagccatt
    24601 catcattcag actgcccgtg tggcgttcta cggcttcggg taatttatcc aggccaggcg
    24661 gtgtcatacg cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc
    24721 aggaaagccc ggtgaggccc agaccgataa tgacgacatt tttaccctga taatcagcca
    24781 tgattaacgt accttcagcg ttgccagacc aatcagaacc agcatcagcg aaataatcca
    24841 gaaacgcaca atgacgcgcg gttccggcca gcctttcagt tcatagtggt gatgaatcgg
    24901 tgccatgcgg aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca ggatgacaga
    24961 aagcgtttct accacgaaca cgccccccat aatcaccagc aggaattcct gacgtagcag
    25021 tacggcgata atgcctaacg caccacctaa cgccagcgaa cctacatcgc ccataaagac
    25081 ctgcgccgga taggtgttaa accacaggaa gcccagtcct gccccgacta tcgccgtgca
    25141 gacaatgacc agttccccgg cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt
    25201 catgttgccg gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat
    25261 aattgccagg ccgtcgagac catcggtcag gtttaccgcg ttgccagtac ccacaatgac
    25321 gaagtaagcc agcagaatgt agaacagccc cagctgcggc atcacatctt taaagaatgg
    25381 gaccaccagc tgcgttgcgg gcgtgtcttt gccggcaagg tacagggcga aggcgacacc
    25441 cagcgcaatg accgacatcc agaaatactt ccaacgagcg atcaaccctt tggtgtcttt
    25501 acgcaccact ttgcgataat catcaacaaa gccaataaca ccgtaaccta ccagcaccac
    25561 caacacgcac cagacgtacg gattggacgg gtaagcccac agcagtacgg agatcactat
    25621 cgccgtcagg atcataatcc cgcccatggt cggcgtaccg cgcttgctga agtgtgattc
    25681 aggaccgtcg ttacgcacca cctgaccaaa ggaaagtttt tgcaaatgag caatcatacg
    25741 cgggcccatc cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt
    25801 cagataggaa aagacgttaa agccggaata atatttgacc aaatgttcgg ccagccaaac
    25861 taacatgtcc cattctcctg taaagcgcgt actacctctt ccatggcggc actacgtgaa
    25921 cccttaacta aaatcgtaat tacctgttgc tcagcaatca gtgatttaag acgcgtaatt
    25981 aacgcagttt tatcagcaaa atgttcgcca acgccgctgg cggtgctgat agcatggctt
    26041 tgtttaccca cgcttaacac gcggtcaata ccagcagctt ttgccgcctc gcccacctgt
    26101 acatggcagg cttcgctttc agcgcccagt tccgccatat cgcccaccac cagcacgcgg
    26161 tagcccggca tttcagccag tacctgaaca gcggcggtca ttgaaccgac attggcgttg
    26221 taggagtcgt cgagcagcaa ctggttttct gccagttgga tggggaacag acggcctgga
    26281 acagctttca gatttgccag ccccgctttg atagcatcaa gcgttgcgcc cacggacatg
    26341 gagagcgcag cggctgccag cgcattcgca atattgtgac gccccggcaa cggcagcaga
    26401 acatcgacgc taccggttgg ggtttgtagg gtaaattccg taccgtgcga ggtcacatgg
    26461 atattggtgg cggtgaaatc gctgttggcg gcattgggtg agaaacgcca cactttgcgt
    26521 gagccaatta cgctctgcca gttcagccag tcgttgttgt cggcgttcat aatggcgata
    26581 ccgttttccg gcaggccgct aaagatttca cctttcgctt tcgcgacacc cgcaagcgag
    26641 ccaaaacctt ccagatgcgc cgctgccagg ttgttgacca gcgcagcttc cgggcgagtc
    26701 agactcacag tccaggctat ttcgccctga tggttcgcgc caagttcaat aactgcgtaa
    26761 tcgtattccg gcgttaagcg caacagcgtc atcggtacac cgatgtcgtt gttgagattg
    26821 cctgccgtat aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg
    26881 gaggttttgc cggaggaccc cgtcagagca accacgcgcg ccggaacttg ctggcgaacc
    26941 catgcagcca gttcaccaaa cgccagacgc gtatccttga cgattaactg cggcaggtcg
    27001 atgtccagcg gacggctaac cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca
    27061 aaatcgtggg catcaaaacg ttcgcctttc agggcaacaa acaggcagcc cggcgtcagt
    27121 tttcgggtat cagtggttac agcatcaagg gtgatatctg caccttgcag ttcaccgttg
    27181 agaatgtcgg taagttggct aagggttacg ctaatcatgc aatcaccccc agcagacgcg
    27241 ccaccgtgac gcgatcggag tagtccagac gctgattgcc aacaatctgg taatcttcat
    27301 ggcctttgcc cgcgaccagt accacatcat tctctttagc ctgcataacg gcgcaagtca
    27361 ccgcttcagc acggccttcc atcactttgg catgtccggc atctaacatt cccgccagaa
    27421 tatcgttgat gatggcacgc ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca
    27481 cgtcagcaaa ctcttcggca attgcgccca tcagtggacg cttaccttta tcgcgatcgc
    27541 caccacagcc aaagacgcac cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg
    27601 ctttttccag tgcatccggc gtatgcgcgt aatccaccac caccgtcggt ttgcctagcg
    27661 cagtgaacac ttccatacgt ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat
    27721 cagccagtgg atagccgagt gccagcagtg tcgccagcgc gagcagcagg ttgctgacgt
    27781 taaaagcgcc catcagatgg ctttcaattt cgccatcgcc ccaacttgag ctaaagcgaa
    27841 tcgtcgcacc gctgtcgtga tagttcacgt cgatcgcttt taaccagcgt ccgtgacagt
    27901 tcggattgat atgatcttcc attgataccg caaccgcgtc cggcagtttt gccagccagc
    27961 ggcggcccac ttcatcgtcg gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa
    28021 gcagccattt cgcagcttcg tagtgttcca tatcaccatg ataatcaagg tgatcgcggc
    28081 ttaagttggt aaagaccgac gccgcaaatt tcaatgccgc cacacggtgc tgtaccagcc
    28141 cgtgggagga aacttccatt gcgcaaaacg tcgcgccctg ctccaccagc cccgccagct
    28201 catgctgaac atcgactgcc gaaccggtag tattttctgt cgggatcact ttcccaagca
    28261 gaccgttacc aacggtgccc attaccgcgc tggtttcgcc aagcagttgg ctccactgcg
    28321 ccagcagctg ggtagtcgtg gttttgccgt tggtgcccgt tacgcctacg agacgtaaat
    28381 tgtcagaggg ttcatggtaa aagcggcccg ccagtgcaga taaacgctcg ttgagctggc
    28441 tgagatagat gaccggtacg ccgtgcattt cacggatttc accatcggtc gcctcatctt
    28501 tcgcctctgc aataatggca gccacacctt gcgctatcgc ctgcgggata tatcgacgcc
    28561 cgtccgcctg atgacctact acagctacaa agagatcgcc cgccgcagcc acacggctgt
    28621 cgagtgtcat ctctcgcagt gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt
    28681 cgcgcaaatt acgatctgcc acctgtcccc tcgccttgat taatcacaaa ttcattttta
    28741 tcgcccgttg tcagcgcatc cggctcgatg ttcatggtac gcaatacgcc gcccatgatg
    28801 gcaccaaaga ccggcgcgga aacggcgccg ccgtagtatt tacccgcctg cggatcgttg
    28861 agaacaacaa ccagcgcgaa gcgcggctga ctcgcaggcg caacgcctgc ggtataagca
    28921 atatatttat tgatgtagcg accgtccggc ccgacctttt tcgcggtacc ggttttaatg
    28981 gcgatacgat agcctttaat cgccgctttc acgccgccgc cgcctggtag cgccacgctt
    29041 tccatcatat gcaccacggt gcgaacaatg gattccggga agacacgttc accgggaacc
    29101 ggggggtcaa ctttggtaat cgacagtggg cgataaatgc cgtagctgcc gatagttgcg
    29161 tagactcgcg ctaactgtaa cggtgttacc attagcccgt agccgaaaga gaaggtggcc
    29221 ctctctatgt cagaccaccg ttgtttttga ggatataagc cactgcgttc tccgaccaac
    29281 cccaaattgg tcgcttttcc cagtccaaaa cgtgagtaag tatctactaa cgctgaggac
    29341 ggcatcgcta acgccagctt ggaaacaccg acgttactcg acttctgtaa taccccggtc
    29401 agggttaatt tgctgtagcg tgccacgtct ttgatttcgt ggccgttaat tcgatagtta
    29461 attcgataag gaatggtatt gagtaccgag ttttcccgca ccacgccacg ttgcaacgcg
    29521 gtcattacca ccatcggttt aaccgttgag cccggttcaa acacgtcggt gatggtacgg
    29581 ttacgcatcg cctctttcgg cgtgccgctc agattgttag ggttgtatga cgggctgtta
    29641 gccatcgcca gcacttcacc ggtgttgaca tccaccagca cggcgctacc ggattcagcc
    29701 ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca gcgcctgcaa gcgttcatca
    29761 atactcaacg ccaggttgtg cgccgcctgg ctgtcagtag aagaaatatc ttcaattacg
    29821 cgaccatagc ggtctttacg cacaatgcgc tcacccggct gcccggtaag ccatttatcg
    29881 aaactcttct caacgccctc aatcccctga ctatcgacgt tagtaaagcc gatgaggtga
    29941 gcagtcactt cgccggatgg ataataacgg cgagattctt cacgcagatg aatccccggc
    30001 agtttcaatt ttttgatgta gtccgccatg tcagggttta cctgacgcgc cagataaata
    30061 aagcgccctt tcgggttggc gttaatgcgg gcagaaagct gatccagcgg aatattgagc
    30121 gcgttagcca gcgccttcca gcggtcaccg acgctgatac cgccagcgtc atgcacttct
    30181 ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg ctaacgggcg accagaacgg
    30241 tcagtaatca tgccgcggga ggtggaaact tgctgaacgc gaagagaacg catgtcgccc
    30301 tctttcacca gcatatccgg ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa
    30361 gccagcgcca ggagaataca gccgcataac aacgcaaaac gccaactaat aaagttggca
    30421 tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat
    30481 ccttattttt gcactacgat attttcttgt gacggatcaa catgctgcat ttgcagcttt
    30541 tccgtggcga tcctttccac ccggctatgg tcgccgagcg cattctcttc aaggatcagg
    30601 ttgcgccatt caatgtctaa agcatctcgc tccagcacca gttgttcgcg ctgagcggtc
    30661 agtaaacggg tatggtgcgc cgtggttacc acggtcaccg ccgtcaaaat aatgcaaatg
    30721 aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt caccgataac accaggcaat
    30781 gcatggcgct cgtggcttcc catcgatcct ttaactttgc ttagagcttc tgtcactctg
    30841 ctgatcatgc gttcgtcctc tctgcgatac gcagaactga actacgggca cgaggattct
    30901 cagccacctc ttcttcgccc ggcattaact tgcctaatgc tcgcagctga cggccaccca
    30961 gttttttgag ctgctcttca gtcatcggta accctgccgg aacttgcgga ccgcggctgt
    31021 tttcacgcat aaaacgtttc acaatacggt cttccagcga gtggaagctg atgatcgaaa
    31081 gccgcccacc cggggccagc acgttgagcg agctttttag cgcctgctct atctcctcca
    31141 gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg agtcgcggga tgtttaaact
    31201 tatctttcac cggcgttgca gcagccacga cttccgccag ttctttggtg cgggtcatcg
    31261 gctgttcgcg attacgctcg acaatggcgc gggcaatgcg tttggcaaaa cgctcttcac
    31321 cataggtttt caatacccag gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg
    31381 ctgactgccc acgggttggg tccatacgca tgtccagcgg accatcgcgc ataaaggaaa
    31441 agccgcgctc agcatcatca agttgcggtg aagagacgcc aagatcgagg agaatgccgt
    31501 cgatcttgcc gataagatcg cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt
    31561 ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc
    31621 gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga gaggatcaga cgtgagtgac
    31681 caccgcgacc aaaagtccca tcaatgtaga tgccatcagg acggatattg aggccattaa
    31741 cggcttcatc cagcagcacc gtagtatgtt tatagttttc catcatttta tagagacaag
    31801 tcctgcagtc gctccgataa gtctccggta gccaactgct ctgcgtcgat atcttccttg
    31861 acctgttgat gccaggttgt ttcatcccac agctcaaact tgttgaactg tccaaccagc
    31921 atcacttctt ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa caatcgacct
    31981 gcgccatcca tctgacattc gctggcatga cctaacagta ggcgctgcac acggcgctca
    32041 accgggttca tgctcgacag acgcgataat ttttgctcga taatttccca ttcaggcagg
    32101 gggtaaagca gcaggcacgg gtgatgaatg tcaatggtgc aaaccatttg accggcagcg
    32161 ttctcaagca gctgttcccg gtaacgggta ggcactgata agcgcccttt gctgtcgaga
    32221 ttgactaacg ttgctccccg gaacatgcca gcctcacccc ttattcccac tttgccccac
    32281 aatttcccac tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg
    32341 cataccgagg taaaaaaccc gatgtttacg gctaatatca gcgttgttta ttttacgtcc
    32401 aacgtgcgca gcaaattaac gttatgaata gctgtaagaa aaaatccctc atcgtcatca
    32461 ccgttttaaa atgactcact attttccaca gcaaatataa agtgtcagtt tgcgacgcga
    32521 gcggcatttt aggacatatc ttccccggtt aacagtccct gttgcgtctg tgtggcgctt
    32581 gatgccagcg ggtttgcgca aaagcaaaga aaagtgcttg ttaaatcggc aattcatctc
    32641 tgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc acttaattat tgtgaatttc
    32701 agctggtgag ttaattaacg ggcgggttta aaagaactta tcaataatag tcaggaatag
    32761 tcttatttac tttaaggata ttgatgtcca ttcccgtact ctacgcgcca gagggaaatt
    32821 cacctggcgc gtatttttgt tcgcggctta gctacggctg agcacaccgc gacgatagag
    32881 attacgttta atgcgcgtta aaccaggttt tggcttacgc ggttcgtcca ggcttgccag
    32941 gacaatctcc agcacacgct ctgcgacatc gcggtgacgt tgagccactg ccagcaccgg
    33001 gcactgtaag aagtcgagca gttcgttatc gccaaaggtg gcaattgcca ggtcagaagg
    33061 cagtttacca tcgcgacgta gcgttacatc catcacccct tgcaacaacg caaacgacgt
    33121 tgtgaacagc gcctgcggca tcggatgcgt ttccagccat ttttcgaata actgggcagc
    33181 cgcctcccgc tcatagctgt tggcatacag gaaatgcact tcgcgcggat catctttcca
    33241 ggcagtacgg aaaccttgtt cacgcaggaa gctgacagaa agctccggca gcgcgccaag
    33301 ataaagcacc gtctcggcgg gaaacttacg taactcttcc gccagcattt cggcatcatc
    33361 ctgatcggca ccaaccacgc tggtgaagtg ttcacgatcg agggcgcggt ccagcgcgac
    33421 aatcgggaac gggtcgttag cccagcgttg ataaaaagga tgctcaggag gcaacgacgt
    33481 cgaaacaata atggcatcga cctgacgctg taaaaggtgc tcaatacagc gcatttcgtt
    33541 gtctggctga tcttctgagc aggcaattag cagttgataa ccccgttgcc gcgcctggcg
    33601 ttcaagatag ttagcgatgc gggtatagct ggtgttctcc agatcgggga tcacaagacc
    33661 aatagaacgt gtgcgtccag cacgaagccc agctgccacg gcgttcgggt ggtaattgtg
    33721 ctcacgcacc acagccatga ctttttcaac ggttttgtcg ctcacacggt attgcttcgc
    33781 tttgccgtta ataacatagc ttgcagtggt ccgcgacact cccgccagcc gagcgatttc
    33841 atccagtttc acaattgccc cttgcgtaaa atgtaaaaac catagccatt gtactggtat
    33901 gggttaaatt cattaacatc taagcgcaga aaattaactg cggcaactgc ttttattcca
    33961 ggttcccact gatttcgcaa aaaaagccca acgtgacatg ttgggctgca aattgcgcac
    34021 tgagatcatt ctcaacgcat tattttatcg ccgcgcgaaa gtccgaccac accagagcga
    34081 gcaacttcca caattttcgc cacatcgcga atcgatgcta aaaatgcatc aagcttatcg
    34141 ctggtgcctg ctaattgaac ggtataaagc gagggggtga catcgataat ttgcccacgg
    34201 aatatttccg tattacgttt cacttcgtca cgcccgtaac cgctggcctg aattttcacc
    34261 agcatgattt cccgctcaac atgcgcgccc tgccccaact cactcacgcg caagacatcg
    34321 accagcttgt gtaattgctt ttcgatctgc tcaagcactt tttcatcgcc cacggtctgg
    34381 atggtcatac gcgataatgt cggatcgtcg gttggcgcaa cggtcaggct ttcaatgttg
    34441 tagccacgct gggaaaaaag gccaatcacg cgggataacg cgcctgattc attttcgagt
    34501 aagactgata atatccggcg cataatcagg ttctctccgt tttgcttaac cacatttcat
    34561 ccattccgcc cccgcgaatc tgcatcgggt agacgtgctc actgccatcg acggtaacat
    34621 caacaaacac caggcgatta ttgcgcacct gttccagcgc ctcgctaagt ttgctttcca
    34681 gctcatgcgg atgagagatc tggatcccga cgtgaccata ggcttccgcc agacggacga
    34741 aatcgggtag tgattgcata taagattgtg aatgacggcc ggaatagatc atgtcctgcc
    34801 actgtttcac catccccaga tagcggttat tgagattcac caccagtacc ggcaactcgt
    34861 attgcaacgc ggtagacagt tcctggatgt tcatctgaat actgccgtcg ccagtgacgc
    34921 aaaccacggt ttcttctggc aacgccattt tgacgcccag tgccgcaggt aaaccaaaac
    34981 ccatcgtgcc gaggccaccg gaattgatcc agcgacgcgg tttgtcgaat ggataataaa
    35041 gtgcagcaaa catctggtgc tgcccgacat cggacgtcac gtaagcgtct cccttcgtca
    35101 accgccaaag agtctcgatc accgcctgcg gtttaatctt ttcactgtga gtgtcatatt
    35161 tcaggcactg acgagcgcgc cactgttcaa tttgctgcca ccagtcgcgg atctcatcca
    35221 gtggttgatg ggcggattct tgcgacaaga gttcaagcat ttgttcgagg acctggcgag
    35281 catcccccac aatcgggata tccgcagtca cggttttaga aatggaagta ggatcaatat
    35341 cgatatgcag aacagtggca tttgggcagt actttgccag attgttcgtc gttcggtcat
    35401 caaatcgtac cccgacggcg aaaatcacat ccgcgttatg catcgtcata ttggcttcgt
    35461 aggtaccgtg cattcccagc atgcccagtg cctgacgatg cgttgccgga aacgccccca
    35521 gccccatcaa tgaggaaaca acgggcagat tcaacgcctc caccgtttct ttcaactgct
    35581 gatggcagcc cgccatgatt gccccaccgc ctacgtagac aaccggtttt tttgccgcta
    35641 ccagcgtttg cagagcgcgc ttaatttgcc ctttatgtcc ggtagtagtg ggattgtaag
    35701 aacgcatact gaccgactca ggccagacat agggtaattt gttcgccgga ttaagaatat
    35761 ctttcggtaa atcaacgacc actggtcctg ggcgaccact tgccgccagc cagaaagcct
    35821 ttttcagcac ctgcggaatg tcttccgttt gcttaaccag aaaactgtgt ttaaccaccg
    35881 gtcgcgaaat ccccaccatg tcgcactcct gaaaggcatc gtaacctatc aacgaggtcg
    35941 ctacctgccc ggaaaggaca actaatggaa tggaatccat ataagcggtg gcgatgccgg
    36001 taatcgcatt ggtcgcccct ggacccgacg ttaccagcac gacgccgact tccccggtcg
    36061 cgcgcgccag accatcggcc atatgcactg ccgcctgctc atgacgaact aaaacatgat
    36121 cgataccacc cacggtatgc aatgcatcgt aaatatcaag gactgcgcct ccgggataac
    36181 cgaatacttg tttaacgccc tgatcgataa gcgatcggac gaccatctcg gctccagaca
    36241 acatctccat ggcctgcctc actgtttgac ggaaaaaatg tgtaaaacac attaaccgtc
    36301 cgatcttgct taagcaattg gcagccagag acataaacgc agggtaataa gaataaacag
    36361 gaggaaaggt gaaaagaaac gataaaatcc tccattacga aggattaaat tgataataaa
    36421 tcggctgaat cccacaactt acagca
//
LOCUS       DAFNBJ010000027        36440 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000027 DAFNBJ010000000
VERSION     DAFNBJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.027"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            53..514
                     /locus_tag="L2X49_001353"
     CDS             53..514
                     /locus_tag="L2X49_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegJ family protein"
                     /protein_id="HBN4796465.1"
                     /translation="MKKILLMLSLLFFTTAGFSEVSDTLVTGGYDKQAMSDAIKHARK
                     ETDKFIEVMNKKDADTFAVKAPITDHGRTEHFWLTDVTYSNGMFIGVISNDPSIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
     gene            complement(579..1340)
                     /gene="pphC"
                     /locus_tag="L2X49_001354"
     CDS             complement(579..1340)
                     /gene="pphC"
                     /locus_tag="L2X49_001354"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HBN4796466.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWAE"
     gene            complement(1337..1996)
                     /locus_tag="L2X49_001355"
     CDS             complement(1337..1996)
                     /locus_tag="L2X49_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBN4796467.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIA
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(2217..2276)
                     /gene="ibsA"
                     /locus_tag="L2X49_001356"
     CDS             complement(2217..2276)
                     /gene="ibsA"
                     /locus_tag="L2X49_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HBN4796468.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(2549..2605)
                     /locus_tag="L2X49_001357"
     CDS             complement(2549..2605)
                     /locus_tag="L2X49_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBN4796469.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(2877..2933)
                     /locus_tag="L2X49_001358"
     CDS             complement(2877..2933)
                     /locus_tag="L2X49_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBN4796470.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            3212..4459
                     /gene="mdtA"
                     /locus_tag="L2X49_001359"
     CDS             3212..4459
                     /gene="mdtA"
                     /locus_tag="L2X49_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBN4796471.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            4459..7581
                     /gene="mdtB"
                     /locus_tag="L2X49_001360"
     CDS             4459..7581
                     /gene="mdtB"
                     /locus_tag="L2X49_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBN4796472.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            7582..10659
                     /gene="mdtC"
                     /locus_tag="L2X49_001361"
     CDS             7582..10659
                     /gene="mdtC"
                     /locus_tag="L2X49_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBN4796473.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRSSLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSMPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLNWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            10660..12075
                     /locus_tag="L2X49_001362"
     CDS             10660..12075
                     /locus_tag="L2X49_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4796474.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            12072..13475
                     /gene="baeS"
                     /locus_tag="L2X49_001363"
     CDS             12072..13475
                     /gene="baeS"
                     /locus_tag="L2X49_001363"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBN4796475.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            13472..14194
                     /gene="baeR"
                     /locus_tag="L2X49_001364"
     CDS             13472..14194
                     /gene="baeR"
                     /locus_tag="L2X49_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBN4796476.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            14385..14717
                     /locus_tag="L2X49_001365"
     CDS             14385..14717
                     /locus_tag="L2X49_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBN4796477.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            14926..15222
                     /locus_tag="L2X49_001366"
     CDS             14926..15222
                     /locus_tag="L2X49_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HBN4796478.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            15224..15520
                     /locus_tag="L2X49_001367"
     CDS             15224..15520
                     /locus_tag="L2X49_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBN4796479.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            15623..16984
                     /gene="yegQ"
                     /locus_tag="L2X49_001368"
     CDS             15623..16984
                     /gene="yegQ"
                     /locus_tag="L2X49_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBN4796480.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(17314..17631)
                     /locus_tag="L2X49_001369"
     CDS             complement(17314..17631)
                     /locus_tag="L2X49_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796481.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            18046..18945
                     /gene="yegS"
                     /locus_tag="L2X49_001370"
     CDS             18046..18945
                     /gene="yegS"
                     /locus_tag="L2X49_001370"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBN4796482.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(19027..19800)
                     /locus_tag="L2X49_001371"
     CDS             complement(19027..19800)
                     /locus_tag="L2X49_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBN4796483.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19906..20946)
                     /gene="gatD"
                     /locus_tag="L2X49_001372"
     CDS             complement(19906..20946)
                     /gene="gatD"
                     /locus_tag="L2X49_001372"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBN4796484.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(20995..22350)
                     /locus_tag="L2X49_001373"
     CDS             complement(20995..22350)
                     /locus_tag="L2X49_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBN4796485.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(22354..22638)
                     /gene="gatB"
                     /locus_tag="L2X49_001374"
     CDS             complement(22354..22638)
                     /gene="gatB"
                     /locus_tag="L2X49_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBN4796486.1"
                     /translation="MKRKIIVACGGAVATSTMAVEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(22669..23121)
                     /gene="gatA"
                     /locus_tag="L2X49_001375"
     CDS             complement(22669..23121)
                     /gene="gatA"
                     /locus_tag="L2X49_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBN4796487.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(23131..24393)
                     /gene="gatZ"
                     /locus_tag="L2X49_001376"
     CDS             complement(23131..24393)
                     /gene="gatZ"
                     /locus_tag="L2X49_001376"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBN4796488.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDTANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(24422..25276)
                     /gene="gatY"
                     /locus_tag="L2X49_001377"
     CDS             complement(24422..25276)
                     /gene="gatY"
                     /locus_tag="L2X49_001377"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBN4796489.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(25586..26638)
                     /gene="fbaB"
                     /locus_tag="L2X49_001378"
     CDS             complement(25586..26638)
                     /gene="fbaB"
                     /locus_tag="L2X49_001378"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBN4796490.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            26895..28172
                     /locus_tag="L2X49_001379"
     CDS             26895..28172
                     /locus_tag="L2X49_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HBN4796491.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            28169..29173
                     /locus_tag="L2X49_001380"
     CDS             28169..29173
                     /locus_tag="L2X49_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBN4796492.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREVV
                     "
     gene            29170..30135
                     /locus_tag="L2X49_001381"
     CDS             29170..30135
                     /locus_tag="L2X49_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBN4796493.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAILLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(30109..30855)
                     /locus_tag="L2X49_001382"
     CDS             complement(30109..30855)
                     /locus_tag="L2X49_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN4796494.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(30907..31725)
                     /locus_tag="L2X49_001383"
     CDS             complement(30907..31725)
                     /locus_tag="L2X49_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBN4796495.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYSYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(31790..32590)
                     /gene="thiD"
                     /locus_tag="L2X49_001384"
     CDS             complement(31790..32590)
                     /gene="thiD"
                     /locus_tag="L2X49_001384"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBN4796496.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(32587..33375)
                     /gene="thiM"
                     /locus_tag="L2X49_001385"
     CDS             complement(32587..33375)
                     /gene="thiM"
                     /locus_tag="L2X49_001385"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBN4796497.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVATCCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(33410..33506)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(33598..33870)
                     /gene="rcnR"
                     /locus_tag="L2X49_001386"
     CDS             complement(33598..33870)
                     /gene="rcnR"
                     /locus_tag="L2X49_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBN4796498.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLIREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            33991..34320
                     /gene="rcnA"
                     /locus_tag="L2X49_001387"
     CDS             33991..34320
                     /gene="rcnA"
                     /locus_tag="L2X49_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux transporter RcnA"
                     /protein_id="HBN4796499.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRT"
     gene            34348..34815
                     /gene="rcnA"
                     /locus_tag="L2X49_001388"
     CDS             34348..34815
                     /gene="rcnA"
                     /locus_tag="L2X49_001388"
                     /inference="COORDINATES: protein motif:HMM:NF007454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux transporter RcnA"
                     /protein_id="HBN4796500.1"
                     /translation="MHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHANDIKR
                     RFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLALTL
                     VTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            35034..35372
                     /gene="rcnB"
                     /locus_tag="L2X49_001389"
     CDS             35034..35372
                     /gene="rcnB"
                     /locus_tag="L2X49_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBN4796501.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(35454..>36440)
                     /locus_tag="L2X49_001390"
     CDS             complement(35454..>36440)
                     /locus_tag="L2X49_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HBN4796502.1"
                     /translation="VSYAACYSELSVQHNLVVQGDFALTQTQMATYEHNFNDSSCVST
                     NTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTSFTSGYSVTVTPAASNA
                     KVNVSAGGGGSVMINGVATLSSASSSTRRSAAVQFLLCLLGGKSWDACVNSYRNALAQ
                     NAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATINSKQGDIILRCK
                     NLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFILESNGSSVTLLNITNSS
                     KGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALINVKYD"
BASE COUNT         8761 a         9449 c         9162 g         9068 t
ORIGIN      
        1 tcacttccac aggttttgct aacttttact acacagagat tctggagaaa aaatgaaaaa
       61 gatattactc atgttgagcc tgctattttt tactaccgcg ggctttagtg aagtttcgga
      121 tacgttagtg acaggaggtt atgataaaca agcgatgtct gacgcgatta aacatgcgcg
      181 gaaagaaact gataaattca tcgaggttat gaataaaaaa gatgccgata ctttcgcagt
      241 caaagccccg ataaccgatc acggaagaac cgagcatttc tggctcacgg atgtcactta
      301 ttctaacggc atgtttattg gcgtaatcag caatgaccct agtattgtta caaacgtaga
      361 atatggccag gagtggaaaa tcaagaaaga agatatttca gactggatgt atacgcgcgg
      421 ggataaaatt tacggcggtt ataccattga tcctttattg gtcacctatc caaaagaaga
      481 agccgacgag cttagagcaa aattagttcg ctaatcagcc gttatattta ttgtttaaac
      541 tggcggttga attgttgtct tcaaccgcca tgagaaactc actctgccca cagcgccagt
      601 gctaacgttt tatcatcatc ggtacgctcg tttaccgctg ggctggacaa aaactgcttt
      661 aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc attgaaaaac
      721 ggggtaaaga aaggcacatg cggagaatta tccaacatat ttaacgccag tcgctggata
      781 ccatccgtaa atgctgcaac tttatgcaca cgctcagtgc tggtgaaagt ttccagtctg
      841 gaaacggcat cttcatcggt aatgaagtgc gtcatattgg catattcacc caccatcggc
      901 gtaagcggca attgcagacc gtgaccaaaa tccaccacca cgccgccatc gccaatctgc
      961 ataatcaagg tgccattagc cgatgaaatc aatcccagaa acgtgcaggc aaaatcgcga
     1021 accgctaatt ctttggcctc tgcctcggca aaaagtcgct gacgaatagt cagcacaata
     1081 tccgtggcga ggatatcatt aagccccaat tccccaccct gcactttttg cgacatataa
     1141 gccatcgctt cattgacggc gagcatcgcg ccttcgccac cctgcgagac gctgcctgca
     1201 ccatcagcaa gaaacatcac cagtaatggt tgctgatcgt ttaaccaggc aacctgcatc
     1261 tggcaggcat cctgacaagg taaatcagcg ctaatgtgcg acgttccaac agcggatgca
     1321 taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc acaacttccg
     1381 ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta aacagttcac
     1441 ggaattgtaa tccttgcaga ggcaaaggct gacgaacgct gatttgtgcc agcgtcttca
     1501 tatccgcccc ctgaacagcg atggaaaaga aggcaaactt cttatcttct tccccctgaa
     1561 acactttgtt ggcggcagcc tgccactcat cggttggagc gccatcggtg atcaggaaaa
     1621 tccacgggcg ataatacgaa ataccatttg cgcggtactc acgtttcctc tcttcaacca
     1681 tatccagggc tttggtaatg gcggccccca ttggcgtatc cccctgggca aacaggatgg
     1741 gcgggaaaaa gttagcggcg ctggtaaagg gttgttccac atgcaccggg ccgaacgtca
     1801 caataccgag ttccactctt tttaaggcca gcgaatcggc aagcagctca tcgcgaaagg
     1861 taaccaatcc ggcgttaagt tcattaatgg gtctgccgct catcgagcca gaaacatcca
     1921 gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg gtggcaaacg
     1981 tgatttgttc gctcataaga aatcctttct tagagaatta ttgaggtccg gtatcatacc
     2041 gactgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc cggcggcgca
     2101 gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact aagggcggag
     2161 tgacataatt tcaggagtga gggttaggga gaggtttccc cctccccctg gtgttcttag
     2221 taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt catcataacc
     2281 ctttccttat taaaagccct cttctccggg agaggctttc ccgtttcagc gtcccgctga
     2341 aatcatcggc ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg tgaataaaat
     2401 agtttcctct gtgattattg cgtaacgact cgcaaagaat atttgctggc aggatcgcag
     2461 actacaaagc ctgcggattg acaatcttat cgtgaaggca tactttcagg agtgagggta
     2521 gagcggggtt tcccccgccc tggtagtctt agtaagcggg gaagcttatg actaagagca
     2581 ccacgatgat gagtagcttc atcatgaccc tttccttatt taaggcccct tcctcgggag
     2641 gggctttccc gtttcagcgt cccgctgaaa tcgtcggctt acctcctttc gccatgcact
     2701 tagtctatcg ctgacttgca gataaaatag tttccagtgt gattattgcg taacgtctcg
     2761 caaagaattt ttgctggcag gatcgcagac ggcaaagcct gcggattgac aatcttatcg
     2821 tgaaggcata ctttcaggag tgagggtaga gcggggtttc ccccgccctg gtagtcttag
     2881 tatgctgggt agctaatcac taagagcacc acgatgatga gtagcttcat catgaccctt
     2941 tccttattta aggccccttc ctcgggaggg gctttcctgt ttcagcatcc ggctgaaatc
     3001 tgcggcttac ctcctttcgc cctgtatgca gtctatcgct gacgcgtaga taaaatagtt
     3061 tcctgtgtta ttactggatg cgtgctcgca aatgtgcccg ccattcagac gattcccgat
     3121 agtgtttcat aattcctcca tttttctccc ttattggctg gctacactag tatcattccg
     3181 cgaaacgttt caggaagaga aactcttaac gatgaaaggc agttataaat cccgttgggt
     3241 aatcgtaatc gtggtggtta tcgccgccat cgccgcattc tggttctggc aaggccgcaa
     3301 tgactcccag agtgcagccc caggggcgac gaaacaagcg cagcaatcgc cagcgggtgg
     3361 tcgccgtggt atgcgttccg gcccattagc cccggttcag gcggcgaccg ccgtagaaca
     3421 ggcagttccg cgttacctca ccgggcttgg caccattacc gccgctaata ctgttacggt
     3481 gcgcagccgc gtggacggtc aactgatggc gttacatttc caggaaggcc agcaggtcaa
     3541 agcaggcgat ttactggcag aaattgaccc cagccagttc aaagttgcat tagcacaagc
     3601 ccagggccaa ctggcaaaag ataaagccac gcttgctaac gcccgccgtg atctggcgcg
     3661 ttatcagcaa ctggcaaaaa ccaatctcgt ctcccgtcag gagctggatg cccaacaggc
     3721 gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa gcaagcgtcg ccagcgcgca
     3781 gctgcaactc gactggagcc gcatcaccgc accagtcgat ggtcgcgttg gtctcaagca
     3841 ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc ggaattgtgg tgatcaccca
     3901 gacgcatcct atcgatttag tctttaccct gccggaaagc gatatcgcta ctgtagtgca
     3961 ggcgcaaaaa gccggaaaac cgctggtggt agaagcctgg gatcgcacca attcgaagaa
     4021 attaagtgaa ggcacgctgt taagcctcga taaccaaatc gatgccacaa ccggtacgat
     4081 taaagtgaaa gcacgcttta ataatcagga tgatgcgctg tttcccaatc agtttgttaa
     4141 cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg atcccaacag ccgccctgca
     4201 aatgggcaat gaaggccatt ttgtctgggt gctgaatagc gaaaacaagg tcagcaaaca
     4261 tctggtgacg ccgggcattc aggacagtca gaaagtggtg atccgtgcag gtatttctgc
     4321 gggcgatcgc gtggtgacag acggcattga tcgcctgacc gaaggggcga aagtggaagt
     4381 ggtggaagcc cagagcgcca ccactccgga agagaaagcc accagccgcg aatacgcgaa
     4441 aaaaggagca cgctcctgat gcaggtgtta cccccgagca gcacaggcgg cccgtcgcgc
     4501 ctttttatta tgcgtcctgt cgccaccacg ctgctgatgg tggcgatctt actcgccggg
     4561 attatcggtt atcgcgccct gcccgtttcg gcgctgccgg aagtggacta tccgaccatt
     4621 caggtggtca cgctctaccc aggtgccagc ccggatgtca tgacctctgc cgttaccgcg
     4681 ccgctagaac gccagttcgg gcagatgtct ggcctgaaac agatgtcgtc gcaaagttcc
     4741 ggcggtgcgt cagttatcac tttgcagttc cagctaacat taccgctcga tgtcgccgag
     4801 caggaagtgc aggccgcgat taacgctgcg accaacttgt tgccgagcga tctgcctaac
     4861 ccgccggttt acagcaaagt gaacccggca gatccgccga tcatgacgct cgccgtcacc
     4921 tcaaccgcca tgccgatgac gcaagtggaa gatatggtgg aaacccgcgt cgcgcagaaa
     4981 atctcgcaga tttccggtgt cggactggtg acgctttccg gcggtcagcg tccggctgtt
     5041 cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc tgaccagcga aaccgtgcgc
     5101 accgccatta ccggcgctaa cgttaactcg gcaaaaggta gcctcgacgg cccttcccgt
     5161 gcggttacgc tttccgctaa cgaccagatg caatccgccg aagagtatcg ccagctaatc
     5221 atcgcctacc agaacggcgc gccaattcgt ctgggcgatg tcgcaactgt agagcaaggt
     5281 gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac aggccattgt gatgaatgtt
     5341 cagcgccagc ccggtgctaa cattatctcc accgccgaca gcattcggca gatgctgcca
     5401 cagctcactg agagtctgcc gaaatcggtg aaggtgacag tgctttccga tcgcaccacc
     5461 aatatccgcg catccgtcga tgatactcag tttgaattga tgatggctat cgcgctggta
     5521 gtcatgatta tctacctgtt tttgcgcaat attccggcga ccatcattcc cggtgttgct
     5581 gtaccgctgt cgttaatcgg cactttcgcg gttatggtgt ttctcgattt ttcaatcaat
     5641 aacctgacac tgatggcgtt aactatcgcc accggattcg tggtcgatga cgccatcgtg
     5701 gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa aaccgttggc ggcggcgctc
     5761 aagggcgcag gtgaaatcgg ctttaccatt atctcgctga ccttctcact gattgcggtg
     5821 ttgatcccac tgctgtttat gggcgatatc gtcgggcgac tgttccgcga atttgctatt
     5881 accctggcgg tagcgatttt gatctcagcg gtggtgtcgc tgaccctgac accgatgatg
     5941 tgcgcgcgga tgctcagcca ggagtcgttg cgtaaacaga accgcttctc ccgtgcctcg
     6001 gaaaaaatgt tcgacaggat aatcgccgcc tatggtcgtg gactggcgaa agtgctgaat
     6061 catccgtggc tgaccttaag cgtggcactc agcacgctgc tgcttagcgt gctgctgtgg
     6121 gtgttcattc cgaaaggttt cttcccggta caggacaatg gcattattca gggcactttg
     6181 caggcaccgc aatccagctc ctttgccaat atggcccagc gacaacgcca ggtcgcggac
     6241 gtgattttgc aggatccggc agtgcaaagc ctgacctcat ttgttggcgt tgatggcact
     6301 aacccgtcgc tgaacagtgc acgtttacaa atcaacctca aaccgttgga tgaacgtgat
     6361 gatcgggtgc aaaaagtcat cgcccgtctg caaacggcgg tagataaagt gccgggcgtc
     6421 gatctcttcc tgcaaccaac gcaggatctg actattgata ctcaggtcag ccgcacccag
     6481 taccagttta ccttgcaggc cacgtcactg gatgcgctca gtacctgggt gccacagttg
     6541 atggaaaaac tccagcaact gccacagctt tctgatgtct ccagcgactg gcaggacaaa
     6601 gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg gatcagcatg
     6661 gcggatgtcg ataacgccct gtacaacgcg tttggtcagc ggctgatttc cactatttat
     6721 actcaggcca accagtatcg cgtggtgctg gagcacaaca ccgaaaatac cccaggcctc
     6781 gcggcgctgg ataccattcg cctgaccagc agcgacggcg gcgtggtgcc gctaagctca
     6841 attgccaaaa ttgagcagcg ttttgcgccg ctctccatca accatctgga tcagttcccg
     6901 gtaacgacca tctcctttaa cgtgccggat aactattcgc tgggcgatgc ggtgcaggcg
     6961 attatggaca ccgaaaagac gctgaatctg ccggtggata tcaccacgca gttccagggc
     7021 agcaccctcg ccttccagtc ggcgctgggc agcactgtct ggctgattgt cgcggcggtg
     7081 gtggcgatgt atatcgtgct cggcattctg tacgagagct ttattcaccc gatcaccatt
     7141 ctctcgacgc tacccaccgc aggggttggc gcactgctgg cgttgctgat tgctggtagc
     7201 gaactggatg tgattgcgat tatcggcatt attttgctga tcggtatcgt gaagaagaac
     7261 gccatcatga tgatcgactt cgcgctggct gccgagcgcg agcaaggcat gtcgccgcgc
     7321 gaggcaatct accaggcttg tctgttgcgt tttcgtccga tcctgatgac cactctggcg
     7381 gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga actgcgtcgt
     7441 ccgttaggta tcggcatggt cggcggtctg attgtcagcc aggtgctgac gctgtttacc
     7501 acgccggtga tttatttgct gttcgaccgc ctggcattgt ggaccaaaag ccgctttgcc
     7561 cgtcatgaag aggaggcgta agtgaagttt tttgccctct tcatttaccg cccggtggcg
     7621 acgattttac tgtcggttgc cattaccctg tgcggcatac tgggcttccg tatgctgccg
     7681 gtcgccccgc tgccgcaggt cgattttccg gtgattatgg tcagcgcctc gctgcccggt
     7741 gcgtcaccag aaacaatggc gtcttccgtt gccacgccgc tggagcgctc acttgggcgc
     7801 attgccggag tcagtgaaat gacctccagc agttcgctcg gcagcacgcg tattattttg
     7861 cagtttgatt ttgaccggga tatcaacggc gcggcgcgcg atgtgcaggc ggcgatcaac
     7921 gctgcacaaa gtttgctgcc cagcgggatg cccagccgtc cgacctatcg caaagctaac
     7981 ccgtcggatg cgccaattat gatcctcacg ctgacatccg atacttattc gcagggtgaa
     8041 ctgtacgatt ttgcctcgac gcagctggct ccgacgattt cgcaaatcga cggtgttggt
     8101 gatgtcgatg tcggcggcag ctcactgccc gccgtacgcg tcgggctgaa tccgcaggcg
     8161 ctgtttaatc agggcgtctc gctggacgac gtacgcactg ccatcagcaa tgccaacgtg
     8221 cgtaaaccgc agggggcgct ggaagatggc actcaccgct ggcagatcca gaccaatgat
     8281 gagctaaaaa ccgccgccga atatcagccg ctgattattc attacaacaa cggcggcgcg
     8341 gttcgtctgg gcgatgtggc gacagtgacc gactctgtac aggatgtacg taacgccggg
     8401 atgaccaatg ccaaaccggc tattttgctg atgatccgca agctgccgga agccaatatt
     8461 atccagacgg ttgacagcat ccgggcaaaa ttaccggagt tgcaggaaac cattccggcg
     8521 gcgattgatc tgcaaattgc ccaggatcgc tcccccacca ttcgctcctc gctggaagaa
     8581 gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc tggtggtgtt tttattcctg
     8641 cgctcgggtc gcgccactat tattcccgcc gtttcgatgc cggtttcgct gattggtacg
     8701 tttgcggcga tgtacctgtg cggattcagt ctcaataacc tttcgttaat ggcgctcacc
     8761 atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc tggaaaacat tgcccgtcat
     8821 ctggaagcgg gaatgaaacc gttgcaagcc gcactgcaag gtactcgcga agtcggtttt
     8881 acggtgctgt cgatgagtct gtcactggtg gcggtgttcc tgccgctgct gttgatgggc
     8941 ggattgccgg gccgactgtt acgcgagttt gccgtgacgc tttctgtcgc cattggtatt
     9001 tcgttgctgg tttctctgac attaacgcca atgatgtgtg gctggatgct gaaagccagc
     9061 aagccgcgcg agcaaaagcg actgcgtggt tttggtcgca tgttggtagc cctgcaacaa
     9121 ggctacggca agtcactgaa ctgggtgctc aatcataccc gtctggtggg cgtggtgctg
     9181 cttggcacca ttgcgctgaa tatctggctg tatatctcga tcccgaaaac cttcttcccg
     9241 gagcaggaca ctggcgtgct gatgggcggg attcaggcgg atcagagtat ttcgtttcag
     9301 gcgatgcgcg gtaagttgca ggatttcatg aaaattatcc gtgacgatcc ggcagtggat
     9361 aatgtcaccg gctttacagg cggttcgcga gtgaacagcg ggatgatgtt tatcaccctc
     9421 aagccacgcg acgaacgcag cgaaacggcg cagcaaatta tcgaccgtct gcgcgtaaaa
     9481 ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg tacaggatat tcgcgttggt
     9541 gggcgtcagt cgaacgccag ctaccagtac acgttgttat ccgacgacct ggcggcactg
     9601 cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt tgccggaact ggcggacgtg
     9661 aactccgatc agcaggataa cggcgcggag atgaatctgg tttacgaccg cgacaccatg
     9721 gcacggctgg gaatcgacgt acaagccgcc aacagtctgt taaataacgc cttcggtcag
     9781 cggcaaatct cgaccattta ccagccgatg aaccagtata aagtggtgat ggaagtggat
     9841 ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt tcgttatcaa taacgaaggc
     9901 aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg cgaatgcccc actatcggtg
     9961 aatcatcagg gattatcggc ggcctcgacc atttcgttca acctgccgac cggaaaatcg
    10021 ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc agcttggtgt gccttcgacg
    10081 gtgcgcggca gttttgccgg aacagcgcag gtgttccagg agacgatgaa ctcacaggtg
    10141 atcctgatta tcgccgccat cgccacggtg tatatcgtgc tgggtatcct ttacgagagt
    10201 tatgtgcacc cgctgacgat tctctccacc ctgccctcgg cgggtgttgg agcgctgctg
    10261 gcgctggagc tgttcaatgc cccgttcagc ctaatcgccc tgatagggat catgctatta
    10321 atcggcatcg tgaagaaaaa cgccattatg atggtcgatt ttgcgcttga agcccaacgg
    10381 cacggtaacc tgacgccgca ggaagctatt ttccaggcct gtctgctgcg ttttcgcccg
    10441 attatgatga ctaccctggc ggcgctgttt ggcgcgctgc cgctggtgtt gtcgggcggc
    10501 gacggctcgg agctgcggca acccctgggg atcaccattg tcggcggact ggtaatgagc
    10561 cagctcctta cgctgtatac cacgccggtg gtgtatctct ttttcgaccg tctgcggctg
    10621 cgtttttcgc gtaaacctaa acaaacggta accgagtaaa tgacagatct tcccgacagc
    10681 acccgttggc aattgtggat tgtggctttc ggcttcttta tgcagtcgct ggacaccacc
    10741 atcgtaaaca ccgcccttcc ctcaatggcg caaagcctcg gggaaagtcc gttgcatatg
    10801 cacatggtca ttgtctctta tgtgctgacc gtggcggtga tgctgcccgc cagcggctgg
    10861 ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg ccatcgtgct gtttactctc
    10921 ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac tgttgctggc acgcgcgtta
    10981 cagggcgttg gcggcgcgat gatggtgccg gtcggcagat tgacggtgat gaaaatcgta
    11041 ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt tacccggtca ggtcggtccg
    11101 ctgctcggtc cggcgctcgg cggtctgctg gtggagtacg cgtcgtggca ctggatcttt
    11161 ttgatcaaca ttccggtggg gattatcggt gcgatcgcca cattgatgtt aatgccgaac
    11221 tacaccatgc agacgcggcg ctttgatctt tccggctttt tattgctggc ggttggcatg
    11281 gcggtattaa ccctggcgct ggacggcagt aaaggtacag gtttatcgcc gctggcgatt
    11341 actggcctgg tcgcagttgg cgtggtggca ctggtgcttt atctgctgca cgccagaaat
    11401 aacaaccgcg ccctgttcag tctgaaactg ttccgcactc gtaccttttc gctgggcctg
    11461 gcggggagct ttgccggacg tattggcagt ggcatgttgc cctttatgac accggttttc
    11521 ctgcaaattg gcctcggttt ctcgccgttt catgccggac tgatgatgat cccgatggtg
    11581 cttggcagca tgggaatgaa gcgaattgtg gttcaggtgg tgaatcgctt tggttatcgt
    11641 cgggtactgg tagcgaccac gctgggtctg tcgctggtca ccctgttgtt tatgactacc
    11701 gctctgttgg gctggtacta cgttttgccg ttcgtcctgt ttttacaagg aatggtcaac
    11761 tcaacgcgtt tctcctccat gaacaccctg acgctgaaag atctcccgga caatctggcg
    11821 agcagcggaa acagcctgct gtcgatgatt atgcaattgt cgatgagtat cggcgtcact
    11881 atcgccgggc tgttgctggg actttttggt tcacagcatg tcagcgtcga cagcggcacc
    11941 acacaaaccg tctttatgta cacctggctt agcatggcgt tgatcatcgc ccttccggcg
    12001 ttcatctttg ccagagtgcc gaacgatacg catcaaaatg tagctatttc gcggcgaaaa
    12061 aggagcgcgc aatgaagttc tggcgacccg gtattaccgg caaactgttt ctggcgattt
    12121 tcgccacctg cattgtcttg ctgatcagta tgcactgggc ggtgcgtatc agttttgagc
    12181 gtggctttat tgattacatc aagcatggta atgaacaacg attacaactg ttaagtgatg
    12241 cgcttggcga gcagtatgcg cagcatggca actggcgctt cctgcgcaac aatgatcgct
    12301 ttgtctttca gatcctgcgt tcatttgaac acgataattc ggaagataaa cccggcccgg
    12361 gtatgccacc gcacggctgg cgtacccagt tctgggtggt tgatcaaaac aacaaagtgc
    12421 tggttggtcc gcgagcgccg attccacctg acggtacacg gcgacccatt ctggtcaacg
    12481 gtgcggaagt tggcgcggtg atcgcctccc ccgttgagcg gttaacgcgc aatactgata
    12541 tcaatttcga taaacaacag cggcaaacca gctggctgat cgtcgccctg gcaacgttac
    12601 tcgcggcgct cgccaccttt ctgctggcgc gcggtttgct ggcaccggta aaacgacttg
    12661 tcgatggcac gcacaaactg gcggcgggcg atttcactac ccgcgtaacg cccaccagtg
    12721 aagatgaact gggcaaactg gcgcaagact tcaaccagct tgccagcacg ctggagaaaa
    12781 accagcaaat gcggcgcgat tttatggccg atatttcaca cgaactgcgt acgccattag
    12841 cggtgctgcg cggtgaactg gaagccattc aagatggcgt gcgcaaattc acgccggaga
    12901 cggtggcgtc tttacaggcg gaagtcggta cactgactaa actggttgac gatcttcatc
    12961 agttgtcgat gtctgatgaa ggcgctctcg cctatcaaaa agcaccggta gatttgatcc
    13021 cactgctgga agtggcgggc ggcgcatttc gcgaacgatt tgccagccgt ggcctgaaac
    13081 tgcagttttc cctgccagac agtattaccg tatttggcga tcgcgaccgt ttaatgcagt
    13141 tattcaataa cttactcgaa aacagcctgc gctacactga tagcggcggc agcctgaaaa
    13201 tctctgccga gcagcacgac aaaacggtgc gcctgacctt tgccgacagt gcgccaggtg
    13261 tcagtgacga gcagctacaa aaattatttg aacgttttta tcgcaccgaa ggttcccgca
    13321 accgcgccag cggtggttcc gggctggggc tggcgatttg cctgaacatt gttgaagcac
    13381 ataatggccg cattattgct gcccattcgc cttttggcgg ggtaagcatt acagtagagt
    13441 taccgctgga acgggattta cagagagaag tatgaccgag ttaccaatcg acgaaaacac
    13501 accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg cagttgctca ttgattatct
    13561 gcgtgctgcg agctatgcgc cgacgcttat cagccacggc gatcaggtac tgccgtatgt
    13621 gcgccagaca ccaccggatc tgatcctgtt agatctgatg ctccctggca ccgatggcct
    13681 gacgctgtgc cgggaaattc gtcgtttttc tgacattccg atcgtgatgg tgacggcaaa
    13741 aatcgaagag atcgatcgcc tgctggggct ggagattggc gcagatgatt atatctgtaa
    13801 gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc attttgcgcc gttgcaaacc
    13861 gcagcgcgag ttgcagcaac aggatgctga aagcccgttg attatcgacg aaggtcgttt
    13921 tcaggcttca tggcgcggta aaatgcttga cctgacgcct gcggaatttc gtctgctgaa
    13981 aacgctctct catgaaccag gaaaagtgtt ctcccgcgag catttgctca atcatcttta
    14041 tgacgactac cgcgtagtaa ccgaccgcac catcgacagc cacattaaaa acctgcgccg
    14101 caagctggaa tctctcgacg ccgaacagtc atttatccgc gccgtttacg gcgttggtta
    14161 ccgctgggaa gccgacgcct gccgcatcgt ttagttttag cgacatgact ttgttagccg
    14221 gagatggcgt tcagaccaga tccggcaaca ttatcccacg catggtcagc aaactgacat
    14281 tatctctccg ccctggcagg catcagaaaa tcgctcatac tttaatcggt aaacagcacc
    14341 tttagatgct gttttcgata cacaatttca atcaaggagt cattatggct ggttggtttg
    14401 aactcagtaa gagcagtgat aatcagttcc ggtttgtgct aaaagcgggc aatggtgaga
    14461 ctatcctcac cagcgagctg tatacctcaa aagcctctgc ggaaaagggc atcgcgtcgg
    14521 tgcgtagcaa cagcccgcaa gaagaacgct atgagaaaaa aacggcaagt aacggcaaat
    14581 tctatttcaa tctgaaagcc gctaatcatc aaattatcgg ctccagccag atgtacgcca
    14641 ccgcgcaatc tcgtgaaacc ggaattgcct ccgttaaagc caatggcaca agccagacgg
    14701 tgaaagacaa tacgtaatta cgatgccggg cgtgggttgt tggcgtccgg ctttatatcc
    14761 aaataacgct tagttattaa taattaagag taaccgccgc cattaaaata gtgggcactg
    14821 acgagcactc gttgtgcttt ctttgtttac catcccatac ttatcctcct gaacatgttc
    14881 atgtgccaaa atatcttcta atactttcgt gacgctatcc ggtttatgac atacactgat
    14941 gtaagatttt tacttacagg cgggcgtaaa atggccagaa cgatgacggt tgatctcggc
    15001 gatgagttgc gtgaatttat cgagtcctta attgaatccg gcgactatcg cacccaaagc
    15061 gaagtgatcc gtgagtcact gcgattactt cgagaaaagc aagcagagtc tcgcctgcaa
    15121 gcgttacgtg acatgctcgc agaagggttg agtagtggtg aagctcagcc gtgggaaaag
    15181 gacgcgttct tacggaaggt gaaagcaggg ataaggaaat aatgtgcgta ttattaaact
    15241 catgccaaaa gccaatgagg atttagaagg catctggtat tacagctatc accatttcgg
    15301 cgaaccacaa gccgacagat atgtggaaca tctttcagac gttctccaga ttctgagtaa
    15361 caataatata ggtacgccca gacctgaatt aggcgaaggt atttttgttc ttccttttga
    15421 acgccacgtc atttatttcc tgcaatcacc aggggaaatt atcgtcattc gtatccttaa
    15481 ccaaaaccag gacgccacca gacatttaca ctggagctaa acccgccgat tgcgctacaa
    15541 tgcccgccct taaagtgggg gcactcccct aaccgcttca tcaggtgaag cggatctgac
    15601 ctgtcatcag aacgagagaa ttatgtttaa accggaactc ctttccccgg cgggaacgct
    15661 gaaaaatatg cgttacgctt tcgcttatgg cgcagatgct gtttatgcgg gccagccgcg
    15721 ttattccctg cgtgtgcgca acaacgaatt caaccacgaa aatcttcagc tcggcatcaa
    15781 tgaagcccac gcgctgggga aaaagtttta tgtcgtggtc aacattgcac cgcacaacgc
    15841 caagctgaaa acctttatcc gtgacctgaa accggtggtg gaaatggggc cggatgcgct
    15901 gattatgtcc gatccagggc tgattatgct ggtgcgtgag cacttccctg aaatgccgat
    15961 ccacctttcg gtgcaggcta acgccgtgaa ctgggcgacg gtgaaattct ggcagcaaat
    16021 gggcctgacc cgcgtgatcc tctctcgcga gctgtcgctg gaagagattg aagagatccg
    16081 caatcaggtg ccggatatgg agatcgagat cttcgttcac ggcgcgctgt gcatggccta
    16141 ctccggtcgc tgcctgctct ctggctatat caacaagcgc gacccgaacc agggcacctg
    16201 caccaacgcc tgccgctggg agtacaacgt ccaggaaggg aaagaagatg atgttggcaa
    16261 catcgtacac aagtacgagc cgattccggt gcaaaatgtt gagccgacgc tgggtatcgg
    16321 cgcaccaacc gacaaagtgt ttatgatcga agaggcccag cgtccgggcg agtatatgac
    16381 cgcgtttgaa gatgagcacg gcacttacat catgaactcg aaagatctgc gcgccatcgc
    16441 ccatgtagaa cgcctgacca aaatgggcgt gcattcgctg aaaatcgaag gtcgtaccaa
    16501 atctttctac tattgtgcac gcaccgcaca ggtttaccgc aaagctatcg atgacgccgc
    16561 tgcgggaaaa ccgttcgata ccagcctgct ggaaactctg gaaggtctgg cgcatcgtgg
    16621 ctataccgaa ggtttcctgc gtcgtcatac tcacgacgat tatcagaact acgaatacgg
    16681 ttattcagtt tctgaccgcc agcagtttgt tggtgagttt accggtgagc gcaaggggga
    16741 cctcgcggcg gtagcggtga aaaataaatt ctccgttggc gacagccttg agctgatgac
    16801 gccgcaaggc aacattaatt ttacccttga gcacatggaa aacgccaaag gcgaagctat
    16861 gccgatagca ccaggcgatg gttatactgt gtggctcccg gtcccgcagg atcttgagct
    16921 caattacgcg ctgctgatgc gtaatttctc cggggaaacc acgcgtaatc cccacggtaa
    16981 gtgattaatt tcgattattt ttcccggatg gaaaattctt agaaaccgat cacatacagc
    17041 tgcatttatt aaggttatca tccgtttcgc tgaaaaacat aacccataaa atgctagctg
    17101 taccaggaac cacctcctta gcctgtgtaa tctcccttac acgggcttat tttttatgca
    17161 taagccctat ccctggtcac cgtcttccat tgaccacatc gatagaatct cccttcatag
    17221 cacgatgcct ttcacgtaac ggcatcgtgc tcgcacaggt tccggctaag cacaaccaga
    17281 acgcgcatgt ttgacgctta ccaaaaaata ttctcactct ccacatttga atgtcagacg
    17341 agcgacgcca tgtaatcctg caccttctgt cttcaggtca actatctgca tttttttgcc
    17401 ctgagtaaca cagaaatggg ctgcatcatt ttttactata ttttctgcac cagatattct
    17461 gcccctggct aaagaagctt cggcttcggt gtagtattgg ttatcgagtt tacgctgaat
    17521 attactttta tatgcaagac caaatttacc gatacttgtc tcatcattat gcacagcaca
    17581 accagacata ataaaaatac taattaatga tatagcagct atctttttca tctcaccttc
    17641 ccccattaaa taccaacgac actctctagt gtttaaatat aataatgaca tgattattat
    17701 aattgaatag gattataata aatgttctgt acaacatttc ctacataagt aggaattacg
    17761 gacattgagg cccttcaggg taactccatg ggggctttaa tatatttgga atagtatatt
    17821 taagatgcca ctgtttggtt gaatattagg catatgctct tttttgaaat ttatcggtgg
    17881 cagccgttag tattcgctgt ccccattgca agctcctggt ggtaaccact gaatcctcca
    17941 tacttgaact gactttttat cctccgactt tcatcctgtt ctgactccac cttttgtttt
    18001 ctgctctaca ctatctacag accaatcata aaggcacata cgatcatggc agaatttccc
    18061 gccagcttac tgattcttaa tggcaaaagt actgacaatc tacccttgcg cgaagcaatt
    18121 atgctgttgc gtgaggaagg aatgacgatc catgtgcggg tcacctggga gaaaggcgat
    18181 gccgcacgat atgtagagga ggcccggaag ttgggcgtcg caacggtgat tgccggtggt
    18241 ggcgatggca ccattaatga agtttctacg gcgttgattc agtgtgaggg ggatgacata
    18301 cccgcgctgg gaattttgcc attaggaacc gccaatgatt ttgccaccag tgtagggatt
    18361 cctgaggcac tggataaggc gctgaaactg gcaattgccg gtaacgccat tgcgatagat
    18421 atggcgcagg tcaacaaaca aacctgtttt attaatatgg cgacaggcgg atttgggacg
    18481 cgtattacca cagaaacgcc ggaaaaatta aaagccgcgc tgggtggcgt ctcttacatc
    18541 attcatggct taatgcgcat ggatactctg caaccggacc gttgtgaaat ccgcggtgaa
    18601 aactttcact ggcaaggtga cgccctggtc attggtattg gtaacgggcg tcaggccggt
    18661 ggcggtcagc aattgtgtcc gaacgcgtta attaacgatg gcttgctgca actgcgcatt
    18721 tttaccggcg atgaaattct tccggctctc gtatcaacct taaaatctga cgaagataac
    18781 ccgaatatta tcgaaggcgc ttcgtcgtgg tttgatatac aagccccaca cgaaatcact
    18841 tttaatcttg atggcgaacc gttgagcgga caaaactttc atattgaaat acttccggcg
    18901 gcgttgcgtt gtcgattacc accagattgt ccattattgc gttaatcaaa ctattctctc
    18961 ttgaaccagg gagaatccgt aatgccggat gcggtgtaaa taccttatcc ggcatacaga
    19021 acaatactac accgtaatta atttcacccg tgtcttctgc aatgccagtt tatccacctc
    19081 tgatacagca tcatcggtaa taataatgtc aattttttcg attggcagta cctgattaaa
    19141 accgcgacgg ttaaatttcg atgaatcgag aacggcaatc actttattag cggctgtgac
    19201 catcgcaccg ctaacggaat aaccctcatt aaaggtcgta ataccattaa ccgcatcaat
    19261 accatcagca ccgacaaaca ttaaatcggc attaatatcc tgcaatgaac gctcggcaat
    19321 ggaaccatgc atcgagcgcg ttttatggcg gaccgtgcca ccacagacga ccagagtaat
    19381 gtctttattt tcggaaaggg caaacgctgc cgggagactg ttggtaatca ccgtgatatt
    19441 tttagcggtc attaatcctt cggcgataag cattgtggta cttccgctgt cgagaataac
    19501 ggtcatccct tcatggatca ttttgactgc cgcctgcgct attctgtttt ttggcgcgct
    19561 ggcgagctga aatcgctctt taaacccgac ttcctgggtc tcggtttcac tattaccgga
    19621 cattattttc gccgcaccgc catgaaagcg cgtaaccacg cctttttgtt cgagaaagcg
    19681 caaatcggca cggattgtcg cttccgaggc agcaaatact cccgccagat cctgaacaag
    19741 cacggttccc tgttcattca ctaattggat gatcttattc cttcgctcga atgaattcat
    19801 cgtcatttac ccatattcaa ttgtagctag tgtaaacgaa gggggaccat tggtgaatga
    19861 tctgttttgc acaataccgg gccagcatca cgctggcccg cggtttcagg gaatgagcaa
    19921 cactttgccc ggcatggcat tacgagcgat gtcacgcacc gcctgggcga agctttcaaa
    19981 gcttccacgg tgagcgatta atggctccag gcttaactta cgttctgtca gcaaccggct
    20041 cgccgtttcc cactcctgcc ccggccaagg gctggagtag ttcatccaac tgccgataac
    20101 cgtcagctct ttacgcaata ttttgccaaa cgttgccgat gttaaatgca gatcctgatg
    20161 caacgtgccc accagcgcca gttgggcatg aggaccggca atctctaccg ccagttcgac
    20221 agtttgcggt acgccagccg tctcgaggat aagctgatta aagcgcagtt cgcgtaaaac
    20281 gctctgcatt tgcggcgcgc tcatttcgct actgttaaat gtttgcatcg caccgaaaga
    20341 ttttgccagt gccagttttt ctgagctaat gtcgatagcc gtcacactct ttgctcccag
    20401 cgcgacagcg cactgaatag ccagcaggcc aatggttccg gcaccaataa taataacgtt
    20461 tttattctca caaccttgcg ctaaatgaaa agcatgcagt ccaacggtaa tcggctcaat
    20521 aaaagcccca tcctcaatag gcatatccgt gggtagagca aagacatttt ttcgcttaac
    20581 gacaatatat tcagcaaatc caccatcacg ccgcgagcca ataaaatcat attttgcgca
    20641 ctgggaataa aaccctttca aacactctgg acaagtaaaa cagggtaata acggcacaca
    20701 ggcaaccgca tcgccagggt gtaaatcatc aacaccggat cccaccgcat caatatagcc
    20761 gctaaattca tggcctaacg ttattggata ataatgtgca ccatttttaa atatcctggg
    20821 taaatcggaa ccacataagc ccgagctggc aatttttacc cgtacctcat cctgatgttt
    20881 aatttcagga atgacgcttt ctgcaacgcg cacgatacca tcagtatcat tcaccactga
    20941 tttcataaaa actcctgatt gttaaggggg gataaactcc ccctcaggta aaaattattc
    21001 tgcgagaacg actttctctt gtttaataaa gccacgcgct ctacgccagg tcatgaaaat
    21061 accggtcaga taaattgcac cgataataat gaaaccggga atattttgcg gggagaaaac
    21121 ctgaatcagt aaccaggtaa tgggagaacc gccctgatcc attgaagcca ccataccccc
    21181 ggcttttaac gccccagcat tagccgccag ttgggtgtgc aaaccaatag tttgcgtcgc
    21241 gatccacagg gtgatgctca taatgatgac acccgagatt aaggtgcgga acagatttcc
    21301 acgatgcacg gcgaccgcca tcgccacgaa gaagccgatg gtggcaagat cgccaaacgg
    21361 cagcacctga ttacccggca cacaaacagc aattaaaatg gtgagtggga taaaaatcag
    21421 gcttgccgat accaccgccg tatgtcccag cagcaacgcc ggatcaaggc caatcaggaa
    21481 ctcctgaccg ccgaacttcg cctgtaaacg actacgagcc tgcttagcga tgggcgttaa
    21541 accatccatg atgggtttaa tcacccgtgg cattagcagc atcactgccg ccgtttttac
    21601 cgccagctgc aatacacctt tgacatcgta acccgcgagg atgccgataa tcagccccat
    21661 cacaaaaccc acggtgacag gctcgccaaa tggaccaaat ttgcgctgaa tatcgtcggc
    21721 gctaaattta attcggttaa cgcctgggat tttctcgatg atagcatcga ccagcaccgc
    21781 aatcggcccc atatacgccg acgtaccgtg cggaatagca ataccttcca gctcaaagaa
    21841 atttcgggta tcgcgggcaa accagtcgcc gagcttataa acaaacgccg cgtgaattac
    21901 cacacctgcc atccctatca tccatgaacc ggttgccagg tgcagcaacg cgccggtgaa
    21961 ggtcatatgc cagatattcc agatatcaac atttaccacc cgcgtcatac gggtcagtaa
    22021 catcgccacg ttaaccaata tcgcaatcgg aatcgccacc agcgcaattt gcgacgccca
    22081 ggtcattggt gaagagcccg gccagccgac atcgaccaca tgcagattca ggtcgaaatt
    22141 ttccgccatc gctttcgccg ccggaccaat ggaatccagc attaagccaa tcacaaggcc
    22201 aatgccaaca aagccaatcc cgatatgcag acccgcttta aagcaatcgc ctgccttcat
    22261 gcctaatatt ttagaaaaaa taatgatgac aaccggcagc atcactgtag ggccgaggtc
    22321 gagaatataa cgcatgactt ctgaaaacat aggtcacccc tgtaagatag tcagaatttt
    22381 attttgtaat gcttcgatac cgacaccaga aataaaaggc atgccgtgaa ctaacggaat
    22441 atcgccaaaa ctacgatcca ctttggcagt ggtgcatatc aaatgcacac catccatata
    22501 ggtttctatt tcattaaccc gacactggat taattcaaca ggaatattat gattctgaca
    22561 caactcttta atttcttcca ccgccatcgt agaggtcgca accgcgcctc cgcaagcgac
    22621 aataatcttg cgtttcataa atagtccttt ttattgttac agagggattt attcgtctga
    22681 atctaaaaca tactttgtga agtattcctt taactgggtt tcaggaagag tgattagtgt
    22741 ctcgacgata tcgggctgtt gtaacttgcc aaataaacag cgtaaaagtt tcaattgctg
    22801 ctgcggattt tccacaatca acgcaataac caacgatacc gccacgtcgt tatcatcatc
    22861 cgcttgctga aaatgaactt tatttgttgg ccttaacaga taaatggctg acgacttagc
    22921 atgaatcgcc tcacaatgcg gtattgcaat ggcgtgctgc tcaagcatta tcccggtagg
    22981 gaattctgct tctctggcaa ttaatgcctg tggccaggta tcatgaacca cacctttggc
    23041 gagcatctca ttaccgatat gggttaaaac ttcgctacga tcgacaaaag aaattccgct
    23101 acgaacaaac aggttagtca tataccgtcc ttattccgca cagccgtagc gataggcgcg
    23161 caaaacatca taaattttat ccataatcag ctgatgcggt attgctgata attccccgga
    23221 ctgaatgcgt tcaaattgtt tgggaagata ctgactaatc atgcccagtg ggatgtccac
    23281 gccttcaaga ttcaccatca tcgtttcgac gctattttta atccgactat gcggccagta
    23341 ataacgaata cgatccgaca ggctgtaacg aatatccagt aatgaatcgt taaaacccgt
    23401 acgataatat tttttccagt attgcggttc gtcgagcatc acttcttcaa ttaccgccag
    23461 gcaaccgctg cgattttcag gggcgataag ttcctgctca atttgtgcca gtgcaaatat
    23521 cgcctcgcgt aaagcaaagg ttaatgcggg accgactttc aatattgcaa agtgatcgcg
    23581 gactaattcc caataagccg tccgggtctg gtaatcggta gaatgtgctt cataaaccat
    23641 tcgggtgttt tctatccatt gcgccagcgg ctgcgcttcc tgcggctgat aatggataat
    23701 attgctgtga tcaaattcca cgcccggctg caccacgatg gcaatcacgc gtgttaacgc
    23761 ctctgtcagc ccacgggcaa taaaggcctt ttgatgcgta cgtaaagtat tggcggtatc
    23821 ttcaacatgg gtgatgtgta ctgactgaat ggcgctggcc tcaccgcccg gaaccggaac
    23881 ttcggtgcca atgacatagc tcagttgctc acgctggcaa tctgtcgcca cactttccgc
    23941 agcaaagcaa agcacagcag ctcgttccgc aaccgtttct ggtgctaacg gtatgggatc
    24001 ccccgcgcag gacattgacg catcaagatg aattttactg aagccggcac gaacatattc
    24061 ctttaccagc tcgacggatt tttccatcgc cgcatccgca ttttcttgct gccagcagtt
    24121 tggccccaga tgatcgccgc cgagaataat gcgttcgcgt gcaaacccaa ctttatcggc
    24181 aatcgtaaaa acaaattcgc gaaagtctgc cggtgtcatt ccggtataac cgccaaattg
    24241 attgacctgg tttgacgttg cttcaatcag cactttgcgc gtgctgttgc gatcaaatgc
    24301 cagcgccgct tcgataacca acggatgggc agaacagact gaacatatgc cgatatgttc
    24361 accagcttta tgccgggcaa ttaacgtttt catgtttttt ccttattaaa tggcaggtgc
    24421 gttatgccct gccctcgcag ccacaatcgg caatcacttt gctcaccaca tcgcgcattg
    24481 cggatttagc cgactgcaaa taatcccggg gatcggtcgc ttcagggtgc tcggtcaggt
    24541 aattttttaa cgcctgcgag aaggcatttt tcagctccgt tgcaacgttg attttgcata
    24601 tccccagttt gatggtttgc tgaatatcct tagtcgataa ccctgacgcg ccatgcagca
    24661 ccagcggtaa gttcacccac tggcgaatgt tctccagtcg agaaaaatca agcgccggtg
    24721 cgctggcata catcccatga gccgtgccga tggcgaccgc cagggaatca attccggttg
    24781 cctcggcaaa ttcacgcgcc tgagcggggt tggtataaaa agcatcggct tcattgactt
    24841 gcacatcatc ttcctggccg ccaagttgcc ccagctccgc ttcgacgctg acatcaaagc
    24901 gatggcaaaa atccaccacc tctttgaccc gtgaaatatt ttgcgcaaaa ggcaaatgcg
    24961 aggcgtcaat catgactgag cgcacgccag aacgaacctt ctgagcgata tcgtcaaatt
    25021 tcgtgtgatg gtcgagatga attgccagtg gatggtgata ttgcttcgcc atcgcgctga
    25081 ccagcgccaa cagattttct gtaccagcat gagtaaatgt gccaggcgtt ccggcgatga
    25141 tgaccggcgc atgcaggttg gcagcggttt ccaccaccac ttgcatcgtt tcgagattgt
    25201 gaatattgaa tgccggaacc gcataaccgc cgcgctgtgc gttgttcagc atctgctttg
    25261 tcgataccac gtacattttc atatcctgtc gtttgttttc gatttcaaaa tataatgaaa
    25321 tgatttgttt ttaaatgtcg agataacgat cacaaaaacg acaatgtgaa aattgttcga
    25381 ggagtaaaag gcaaaaaaaa acggcctccc gatagggaag ccgtagcaaa gtgcgcgtgt
    25441 ttttatgccg gatgcggtgt aaacatacgt ggcagtgcta tgtaggcatg ataagacgct
    25501 tccgcgtcgc atcaggcata ggttgccgga tgcagtgtaa acgccttatc cgtcctacgg
    25561 ggtggtgctg ttcgtatgag gcgtatcagg caatagtaat tttgctatcg agataaacgt
    25621 cctgcacggc attaatcagt ttcacgccgt cagccatcga tttcttgaac gccttacgtc
    25681 caagaatcag ccccattccg ccagcgcgtt tgttgataac cgcagtacgc actgcatcac
    25741 tgaggtcagt ttcaccgcct gcagcaccgc cggagttaat cagccctgcg cgccccatat
    25801 agcagttagc taactgataa cgcaccagat caatcgggtt ttcgctggtt aacttgctgt
    25861 acacgcgatc gtcggtataa ccgtaattaa ttgctttata gccgccgtta ttttccgcca
    25921 ttttttgttt gacgatatct gcaccgatgg ttgccgccag atggtttgcc tgaccggtca
    25981 ggtcggcgga aacatggtaa tcaacgccat ctttcttaaa ggcggagtta cgcaaatagg
    26041 cccacagcac tgtcaccata cccagctcgt gcgcacgttc aaaagccgca gaaatttctt
    26101 caatctggcg acgtgactct tccgagccaa aatagatagt cgcaccaacc gcaaccgcgc
    26161 ccatgttgaa cgcctgctcc acgctggcat acagcgtttg atcgtaggta ttcgggtaac
    26221 ttagcgtctc gttgtgatta agtttgacga ggaatggaat gcgatgcgca taacgccgcg
    26281 ataccgacgc cagcacgccg taagttgacg ccacacagtt acagcccgct tcgatcgcca
    26341 gttcaacaat gtttttcggg tcaaagtaga gcgggttagc agcaaatgaa gctccggcag
    26401 agtgctcaac gccctggtca accggcagaa tagaaagata tcctgtgcca gccagacgcc
    26461 cggtgttgta caacgtctgc atattacgta acaccgctgg cgggcgatta ttgtcaatca
    26521 ttacgcggtc tacgtagtca tgtccgggga gataaagctg gtcagaagga attgtcatac
    26581 aacggtgctg taaaaggttg tcggcgtctt tgccaagcaa ctgcgcaata tctgtcatta
    26641 ctatgctccc gtaaattccg attggatatc ggctatggat tgtcctggcc cgccttttgc
    26701 gggcaatcat aatcctggtc gttacgggcg agtttttcca tcttttggat gttttttcag
    26761 cgttttctgt tggctcgatt catcagaaaa aatgttagcg cggtcaagtt ttcaccgcaa
    26821 aggtatttaa aaggtattat taagtggtat tgtcatcgcg taccttacat tacctgtcat
    26881 gaaggaatta aaagatgaaa acaacagcaa agctgtcgtt catgatgttt gttgaatggt
    26941 ttatctgggg cgcgtggttt gtgccattgt ggttgtggtt aagtaaaagc ggttttagtg
    27001 ccggagaaat tggctggtcg tatgcctgta ccgccattgc ggcgatcctg tcgccaattc
    27061 tggttggctc catcactgac cgctttttct cggcgcaaaa agtgctggcg gtattgatgt
    27121 tcgcaggcgc gctgctgatg tatttcgctg cgcaacagac cacttttgcc gggttctttc
    27181 cgttactgct ggcttactct ctaacctata tgccgaccat tgcgctgact aacagcatcg
    27241 cttttgccaa cgtgccggat gtggagcgtg atttcccgcg cattcgtgtg atgggcacta
    27301 tcggctggat tgcctccggt ctggcatgtg gtttcttgcc gcaaatactg gggtatgccg
    27361 atatctcacc gactaacatc ccgctgctga ttaccgccgg aagttctgct ctgctcggtg
    27421 tgtttgcgtt tttcctgccc gacacgccac caaaaagcac cggcaaaatg gatattaaag
    27481 tcatgctcgg cctggatgcg ctgatcctgc tgcgcgataa aaacttcctc gtctttttct
    27541 tctgttcatt cctgtttgcg atgccactgg cgttctatta catctttgcc aacggttatc
    27601 tgaccgaagt cggcatgaaa aatgccaccg gctggatgac gctcggccag ttctctgaaa
    27661 tattctttat gctggcatta ccgtttttca ctaaacgctt tggtatcaaa aaggtattat
    27721 tgcttggtct ggtcaccgct gcgatccgct atggcttctt tatttacggt agtgcggatg
    27781 aatatttcac ctacgcgtta ctgttcctcg gtattttgct tcacggcgta agttacgatt
    27841 tttactacgt taccgcttac atctatgtcg ataaaaaagc ccccgtgcat atgcgtaccg
    27901 ccgcgcaggg gctgatcacg ctctgctgcc agggcttcgg cagtttgctc ggctatcgtc
    27961 ttggcggtgt gatgatggaa aagatgttcg cttatcagga accggtaaac ggactgactt
    28021 tcaactggtc cgggatgtgg acttttggcg cggtgatgat tgccattatc gccgtgctgt
    28081 tcatgatttt tttccgcgaa tccgacaacg aaattacggc tatcaaggtc gatgatcgcg
    28141 atattgcgtt gacacaaggg gaagttaaat gaaaacagaa cgtattctcg gtgctcttta
    28201 tgggcaggcg ttaggggatg cgatggggat gccctccgag ctttggccac gcagccgcgt
    28261 taaagcacac tttggctgga ttgaccgttt tcttcctgga ccaaaggaga ataacgcggc
    28321 ctgttatttt aaccgcgccg aattcaccga cgatacctcg atggcgctgt gtctggcgga
    28381 tgcgttactg gaacgtgaag gcaagatcga tccggatctg attgggcgta atattctcga
    28441 ctgggcgctg cgtttcgacg cctttaacaa aaacgtacta ggtccgacct cgaagattgc
    28501 gcttaacgcc attcgcgacg gtaaacccgt tgctgaactg gaaaacaacg gcgtgaccaa
    28561 cggcgcagcg atgcgcgtct cgccattagg ttgtttgctt ccggcgcgtg atgttgattc
    28621 ctttattgat gatgtagcgc tggcgtccag cccgacccat aaatccgatc tggcggttgc
    28681 aggcgcggta gtcatcgcat gggcgatttc tcgtgccatt gacggagaaa gctggtcagc
    28741 gattgttgat tcactgcctt caattgcgcg acatgcccag caaaaacgca ccaccacgtt
    28801 cagcgcctca ctggcggcgc ggctggagat tgcgctgaaa attgtgcgca atgccgacgg
    28861 caccgaatcc gccagcgaac agctttacca ggtcgttggc gcaggtacca gcactattga
    28921 gtccgttccg tgcgccattg cgctggttga actggcacaa accgacccga atcgctgcgc
    28981 cgtcctgtgc gctaaccttg gcggcgacac agacaccatc ggtgctatgg cgacggcaat
    29041 ttgcggcgcg ttgcatggcg ttaacgctat cgatcctgcg ttaaaggcgg aactggatgc
    29101 ggtaaatcag cttgatttca accgctatgc cacagcgttg gcgaaatatc gtcaacaacg
    29161 ggaggtggta tgagcggcgc tcgattacac acgctgctgc ctgaattaac cacgcgtcag
    29221 ccggtgatgg tcgtcggcgc ggcggtcatt gacgtgatcg ccgacgctta tgccctcccc
    29281 tggcgtgggt gcgatatcga actgaaacag cagagcgtta acgttggcgg ctgcgcactg
    29341 aatattgccg tggcgttaaa gcgcctcggc atcgaagcgg gtaatgcctt gccgctcggt
    29401 cagggcgtgt gggcggagat tattcgcaac cggatggcaa aagagggctt aatcagcctg
    29461 atcgataacg ccgaaggtga taacggctgg tgtctggcgc tggttgagcc ggatggcgaa
    29521 cgcactttta tgtcattcag cggtgtggaa aatcagtgga atcgccagtg gctggcgcga
    29581 ttaaccgttg cgcctggcag cctgctctat ttttccggtt atcaactggc ctcgccctgc
    29641 ggcgaattgt tagtggaatg gctggaagag ctgcaagacg tgacgccgtt tatcgatttt
    29701 ggcccacgta ttggcgatat cccggatgca ttactggcgc ggatcatggc ctgtcgacct
    29761 ttagtgtcgc tcaatcgtca agaggctgag attgccgccg aacgttttgc tttatccgca
    29821 gagataacaa cacttggcga gcaatggcag gagaaatttg ccgcgccgtt gatcgttcgt
    29881 ctcgataaag aaggcgcatg gtatttcagc aacgacgctt ctggctgcat tccggcattt
    29941 ccaacgcaag ttgtagacac cattggggcg ggcgacagtc atgcaggtgg cgtacttgcc
    30001 gggctggcct ctggtctgcc actggcggat gccatattac tgggcaatgc agtggcgtcg
    30061 tgggttgtcg ggcatcgggg cggtgattgt gcgccaacgc gcgaggaact actcctcgca
    30121 cacaaaaacg tatagatcgc tgcgacagtg gctaatgctg tactcaatag gccgttgctg
    30181 ttggtcaagc gccacttgct tgatcaccag caccggtatt ttgctatcta actgaatatg
    30241 tgactgaaac tcggcatccg gcatccgggc gctaacgcgg gaacgcgtac gttgtgggta
    30301 aatgtgctgg ctgcggaaat agtcataaag tgaaatcccg atggcatcaa catcatgaat
    30361 taaatgcgcc ggaacccacg attcctcaat cgataccgct tcttcatcga catagcgaat
    30421 acgcttaagc aaaaagacat cacttcctgc ttcgaccgcc agctgctgcg cgacttcttc
    30481 gggacatttc acgacacgct tgttaaccca tagcgtatcg ggctttttcc cgcgcaatac
    30541 cacctgctga gaaaaacctc gcgcttcttt caacgagtat tcgaagatgt tgttgatctg
    30601 cgtgccgtaa ccgcgcgaac gcgtcaccac accctcttct tccagcgcct gcatcgcctt
    30661 gcgcaccgta atgcgcgaaa cgccggttaa ctgacttaaa tcacgctcac cgggcaaaat
    30721 attgccatgc tccagcaccc cgctgcgcac ggcatttttt accgtttcgg caaacttaat
    30781 ataaagcggc gtgttatccg ccgctaaaat acgctcgttc agttgggcaa tcagctgggt
    30841 atgcgcttgt tccatttgtc tttttcctgc cgaggagtct ggtgggagta tatcactgct
    30901 tcttatttaa ggcgtttctt taatcccatc aacgaatgcc tgcaactcct ccaccgtgcc
    30961 atgaaataca ttaaaatcca ccggaccatt gatgccatct acctgtccac ggtcggaatg
    31021 ctgccagaag cgccaggcca tgccgtcatt gtccggacga cgttgataat agtgagccac
    31081 ccaccacgga tattcattga aatagcccgc cagattggtg tgataaaaaa cggctcctga
    31141 gtaaataatc ggcttttttc ccgtactttt ttcgaccatt tttagccact gacttactcg
    31201 tttgcgtaat tcttttgccg ataattttcc ccgttcttct acgtccagta ccgcagggaa
    31261 atcgccttgt gagaaatcca ccgtttgcag aaataatcgt gcctgaaccg gcgctgctac
    31321 cgacggggag aaataatgat acgccccgcg cagcaggcca ttttcgcggc ttagttgcca
    31381 gttacgcgaa aaatacgggt ccaccagctt ttcgccttcc gtcgccttaa taaaagcaaa
    31441 ctgtaagcgg ataccgttgt cgcgcatttt tgccacccgc tgccagtcga tccgctcctg
    31501 ccagcgcgaa acatctattc cgtgaatggt ataactggca ggaatgcgaa ttgcgaaaga
    31561 tttaaccggg cgataactgt agaagtggat gtagtctgta atctgaactg ctgtcgaata
    31621 gaaaaaattc accgtctgac gcggataaat tgcaacgatg gaaattagcc caagagcgca
    31681 taatagagag gtgagttttt ttcgactggt aattcttagc tgcattggtt tcatccctga
    31741 atgtcagtgc caaaggctga caataaccaa agcagctata gtacggtgct caccaccagg
    31801 cgtggaagtg gtgaaccgga ccaataccgt gaccaacttc cagcgtgtcg gcctgggcta
    31861 atgctgatga aagccagctt tttgcctcct gtacggtgtc agcccagttt gtatggcgcg
    31921 ggcgtagtgc agccaacgcc gcagagagcg tacaaccagt gccgtgggtg tttttggtca
    31981 taatgcgcgg tgcggtaaac cgttgttcac cctcgcgggt aaacagccag tccgggcttt
    32041 gctcatcatc cagatgacca cctttcatta gcactgcgcc acagcccatc gccaacagcg
    32101 atcgcccttg ttccagcatt tcctgttcgg tgcgcgcgtg tggcgcgtcg agcaaggcgg
    32161 cagcttcggg caagtttggc gttattaatg aaacctgtgg caataatcga ctgcgcagcg
    32221 tagcaaccgc cgaaggtgaa agcagcgggt cgccgctttt tgccagcata acggtgtcga
    32281 gtaccacgtt ttggatctga taacgttgca accgttctgc caccgcttca acaatatcgg
    32341 tttccgccaa cataccgatt ttagtggtgt cgattcgcac atcgctgaac accgaatcga
    32401 gctgggcggc gacaaaatca ggctcaatgc gatacaccga ctgtacgcca cgggtatttt
    32461 gcgccaccag tgcagtaata actgagcaac cataagcgcc aagtgccgag aaggttttaa
    32521 gatcggcctg aatccccgca ccaccactcg gatcagtacc ggcaatcgtc agagcgttaa
    32581 ttcgtttcat gcctgcacct cctgcgtcaa ttgccagagc gcatcaagga aatgtggaac
    32641 aaaactgcct ggcccctcgc ttctggcgac tgcgcgctct ccggcttgtt tcatccagtg
    32701 acaggcagat gcgacatttt ccagcgtatc gcctggtaac gcacagcagg tagcgacaac
    32761 cgccgataat gcacagccag ttcctaccac tttggtcatt aacggatcgc caccgtgaat
    32821 accaatgata cgatgtccat cggtaacata atccatctcg ccagtgacca cgacgattgc
    32881 gccagtttcc cgtgccagtg tttgtgcagc gggtatcgcg ttagctgcgg cgtcagtggt
    32941 atccactccc cgtccaccat tagcaatgcc agctaatgcc atgatttccg aagcattacc
    33001 acgtatggcg gttggcttat gagacagaag ttccagacaa aaacggcggc gataatcgag
    33061 tgcaccaacc gctactggat caagcgtcca gggtgtttga gagctttttg cttgctcaac
    33121 ggcggcacac atcgactgtg cgcgtagctg cgtcagcgtg ccaacgttaa tcagcaacgc
    33181 actggcaata gccgcaaact gactggcctc ttcggtttcg ataaccatcg ctggcgatgc
    33241 accgagcgcc agcaaggtat tggcagtaaa ggtttgcacc acatcgttgg tcatgcagtg
    33301 cacaagaggg gaatgttggt gaaaaaggtg taacgcgtgc gcagattgcg ctgaactcag
    33361 caggtcgact tgcatagttt gctcctgcca taacgtgaag aagcaatgac ctggtggtcc
    33421 gtgacttccc tacgctggca ttatccagat caggtgatac gggtatttct cagccttcac
    33481 gcagaagggc accccgagtc gtttggttgc gatgataaat cgcagaggag gatggtaatg
    33541 tccagcgcac gcgttgtaaa cgaggaaaaa cggcagtttt acaatcgcgt taataaatta
    33601 tttgatatat gaatccagca ccttcagaac gacatccaga tcttcttcac gttttagctc
    33661 atccccctgg tgaacgatgt gttccgtcag atgaccttta atcacttccc gtatcagacc
    33721 gtttaccgcg ccacggatag cagcaatctg ttgtaaaact gcagcgcatt cgtgcggctc
    33781 gtcgagcatt ttcttgagcg ccacgacctg gccctgaatc ttactggcac gcgctttcag
    33841 tttctgttta tcacggattg tatgagacat ggcaacacct ggttaacaag aatatgaaaa
    33901 atcatagcac tattaatcta ctggggggta gtatcaggta ctggggggga gtagaatcag
    33961 attgccgaat taatactaag aattattatc atgaccgaat ttacaactct tcttcagcaa
    34021 ggaaacgcct ggttcttcat ccccagcgcc atcttacttg gtgcgcttca tggcctggaa
    34081 ccagggcact caaaaacgat gatggcggcg tttatcatcg ccatcaaagg caccattaaa
    34141 caggcggtga tgctcggact ggcagcaact atttcgcata ccgcagtggt ctggttaatt
    34201 gcctttggcg ggatggtgat cagcaagcgc tttactgctc aatcagcaga accgtggctc
    34261 cagctgattt ccgcagtgat cattattggc accgcgttct ggatgttctg gcgtacctag
    34321 cgcggcgaac gcaactggct ggagaatatg cacgagtatg atcatgaaca tcatcaccac
    34381 gatcacgaag atcaccacga ccatggacat catcaccatc acgaacatgg cgagtatcag
    34441 gatgcccatg cacgagccca tgccaatgat attaaacgac gctttgatgg tagagaggtc
    34501 accaactggc aaattttgtt atttggctta acaggtggtc ttatcccctg tccggcagca
    34561 attaccgtgc tgttgatttg cattcagttg aaagccctga cactgggcgc aacactggtc
    34621 gtcagtttca gcattggcct ggcgttaacg cttgtcaccg taggcgttgg cgcagcaatc
    34681 agcgttcagc aggtcgcaaa acgctggagc ggatttaaca ctctcgctaa acgcgccccc
    34741 tatttttcca gtctgttgat tggcttagtc ggtgtgtata tgggcgtaca tggcttcatg
    34801 ggcataatgc gataactcaa tcctttaggc ttgcatgtta tcttcagccc aggatgattc
    34861 ttaaatcagc tatttccgct gactctctgc tcgctaagga tgcttttagg gcatcctttc
    34921 atttacactt tttacgaaat catgggatca ctaacaaaat atcgcttgtc agttatattg
    34981 tatggcagga aagatatgcg actgatatta cagatcccca aagtggagag tttatgacca
    35041 ttaaaaataa gatgttgctg ggtgcgcttt tgctggttac cagtgccgcc tgggccgcac
    35101 cagccaccgc gggttcgacc aatacctcgg gaatttctaa gtatgagtta agcagtttca
    35161 ttgctgactt taagcatttc aaaccaggcg ataccgtccc agagatgtac cgcaccgatg
    35221 agtacaacat taagcagtgg cagttacgta acctgcccgc gcctgatgcc gggacgcact
    35281 ggacctatat gggtggcgcg tacgtgttga tcagcgacac cgacggtaaa atcattaaag
    35341 cctacgacgg tgagattttt tatcatcgct aaaaaaagcc ccctcatcat gagggggaaa
    35401 tgcagacacc ttgttatttt ttattattag ccacttgctc gtcttgcttg ttattagtcg
    35461 tatttcacgt tgattaatgc ggttgcctcc agtgcgccag atttaacttt gtttgtatcg
    35521 tagacgtagt aactggcagt tatcggaatt gaaaccgttg tatctgtaag ttttgacttt
    35581 gccgcaattt cttttaaatt tgtgtatcct ttactgctgt tagtgatatt taaaagtgtg
    35641 actgacgaac cattactttc aagaataaat cctacgccat tatcttccgc acctttcagt
    35701 attgtgttgc tgttggttaa caagtcagaa cttgataaat acacctgcat tttccgtgat
    35761 gtttgatttt gttgacctaa taaattttta caacgcagaa taatatcccc ttgtttacta
    35821 tttattgttg ctttgttacc tgtggctggt aattgtgaaa cgggaatact gtctaaagta
    35881 attaataaat cgtccggttt gcaggttgtg gttatcgggt tgtatgacaa tgtcagatta
    35941 aaggaataga cacctgcatt ttgtgccaat gcatttctgt agctatttac acatgcatcc
    36001 catgacttgc cacctaataa acacagtaga aattgtactg cggcactcct gcgtgtcgat
    36061 gatgaagcac tggataatgt cgcaactcca ttaatcatca ctgaaccgcc gccccccgca
    36121 gaaacattca cttttgcatt actggccgca ggtgtcaccg ttaccgaata accactggtg
    36181 aaactggtct gattatttga taacgtgatg ttgtttttat tggtccattc aaaatgtaaa
    36241 tttaatttaa tggtatcgtt ataaagtcca acaataatat ccgacgggct cataggggtg
    36301 atagtatttg tactcacgca tgacgaatca ttaaaattat gctcatatgt cgccatttgt
    36361 gtttgagtaa gtgcaaaatc cccctgaaca accaaattgt gctgaacaga taactcacta
    36421 taacatgccg catagctcac
//
LOCUS       DAFNBJ010000028        36389 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000028 DAFNBJ010000000
VERSION     DAFNBJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.028"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..1356
                     /locus_tag="L2X49_001391"
     CDS             <1..1356
                     /locus_tag="L2X49_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4796503.1"
                     /translation="KENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAMLL
                     LFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLDRK
                     GPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFNIK
                     ERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYRNR
                     EHVELTAVEQAYLNAGSVNAHRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLYLA
                     WLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKICII
                     AGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASVGS
                     IQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDPRK
                     D"
     gene            complement(1405..3060)
                     /gene="tsr"
                     /locus_tag="L2X49_001392"
     CDS             complement(1405..3060)
                     /gene="tsr"
                     /locus_tag="L2X49_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBN4796504.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(3179..3625)
                     /gene="hpaR"
                     /locus_tag="L2X49_001393"
     CDS             complement(3179..3625)
                     /gene="hpaR"
                     /locus_tag="L2X49_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBN4796505.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3897..5186
                     /locus_tag="L2X49_001394"
     CDS             3897..5186
                     /locus_tag="L2X49_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBN4796506.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            5183..6649
                     /gene="hpaE"
                     /locus_tag="L2X49_001395"
     CDS             5183..6649
                     /gene="hpaE"
                     /locus_tag="L2X49_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBN4796507.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6651..7502
                     /gene="hpaD"
                     /locus_tag="L2X49_001396"
     CDS             6651..7502
                     /gene="hpaD"
                     /locus_tag="L2X49_001396"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBN4796508.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7512..7892
                     /locus_tag="L2X49_001397"
     CDS             7512..7892
                     /locus_tag="L2X49_001397"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBN4796509.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            8003..8806
                     /gene="hpaH"
                     /locus_tag="L2X49_001398"
     CDS             8003..8806
                     /gene="hpaH"
                     /locus_tag="L2X49_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBN4796510.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGTLLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8817..9605
                     /gene="hpaI"
                     /locus_tag="L2X49_001399"
     CDS             8817..9605
                     /gene="hpaI"
                     /locus_tag="L2X49_001399"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBN4796511.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLLQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9780..11156
                     /gene="hpaX"
                     /locus_tag="L2X49_001400"
     CDS             9780..11156
                     /gene="hpaX"
                     /locus_tag="L2X49_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBN4796512.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            11166..12056
                     /gene="hpaA"
                     /locus_tag="L2X49_001401"
     CDS             11166..12056
                     /gene="hpaA"
                     /locus_tag="L2X49_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBN4796513.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            12307..13869
                     /gene="hpaB"
                     /locus_tag="L2X49_001402"
     CDS             12307..13869
                     /gene="hpaB"
                     /locus_tag="L2X49_001402"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HBN4796514.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13887..14399
                     /locus_tag="L2X49_001403"
     CDS             13887..14399
                     /locus_tag="L2X49_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBN4796515.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14792..16942
                     /gene="btsT"
                     /locus_tag="L2X49_001404"
     CDS             14792..16942
                     /gene="btsT"
                     /locus_tag="L2X49_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBN4796516.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFISALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16992..17195
                     /locus_tag="L2X49_001405"
     CDS             16992..17195
                     /locus_tag="L2X49_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBN4796517.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            17206..18162
                     /gene="yjiA"
                     /locus_tag="L2X49_001406"
     CDS             17206..18162
                     /gene="yjiA"
                     /locus_tag="L2X49_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBN4796518.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRSAFAGLKK"
     gene            complement(18240..20781)
                     /locus_tag="L2X49_001407"
                     /pseudo
     CDS             complement(18240..20781)
                     /locus_tag="L2X49_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
     gene            complement(21133..21297)
                     /locus_tag="L2X49_001408"
     CDS             complement(21133..21297)
                     /locus_tag="L2X49_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HBN4796519.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            21474..22886
                     /locus_tag="L2X49_001409"
     CDS             21474..22886
                     /locus_tag="L2X49_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBN4796520.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYCMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGDAVYRAME"
     gene            complement(23129..24085)
                     /locus_tag="L2X49_001410"
     CDS             complement(23129..24085)
                     /locus_tag="L2X49_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBN4796521.1"
                     /translation="MTNFSTSTPHDALFKSFLTHPGTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKREPWIEY"
     gene            24552..25784
                     /gene="mdtM"
                     /locus_tag="L2X49_001411"
     CDS             24552..25784
                     /gene="mdtM"
                     /locus_tag="L2X49_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HBN4796522.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            25825..27105
                     /locus_tag="L2X49_001412"
     CDS             25825..27105
                     /locus_tag="L2X49_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HBN4796523.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            27221..28372
                     /locus_tag="L2X49_001413"
     CDS             27221..28372
                     /locus_tag="L2X49_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HBN4796524.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPPLSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            28382..29149
                     /locus_tag="L2X49_001414"
     CDS             28382..29149
                     /locus_tag="L2X49_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796525.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            29146..29403
                     /locus_tag="L2X49_001415"
     CDS             29146..29403
                     /locus_tag="L2X49_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBN4796526.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            29468..30328
                     /locus_tag="L2X49_001416"
     CDS             29468..30328
                     /locus_tag="L2X49_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HBN4796527.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            30396..31574
                     /locus_tag="L2X49_001417"
     CDS             30396..31574
                     /locus_tag="L2X49_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4796528.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(31587..32141)
                     /gene="kptA"
                     /locus_tag="L2X49_001418"
     CDS             complement(31587..32141)
                     /gene="kptA"
                     /locus_tag="L2X49_001418"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBN4796529.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFVEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            32391..33074
                     /locus_tag="L2X49_001419"
     CDS             32391..33074
                     /locus_tag="L2X49_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796530.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            33071..33532
                     /locus_tag="L2X49_001420"
     CDS             33071..33532
                     /locus_tag="L2X49_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBN4796531.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            33545..34717
                     /gene="iadA"
                     /locus_tag="L2X49_001421"
     CDS             33545..34717
                     /gene="iadA"
                     /locus_tag="L2X49_001421"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBN4796532.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            34782..35693
                     /gene="hypT"
                     /locus_tag="L2X49_001422"
                     /gene_synonym="qseD"
     CDS             34782..35693
                     /gene="hypT"
                     /locus_tag="L2X49_001422"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBN4796533.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(35686..36078)
                     /gene="iraD"
                     /locus_tag="L2X49_001423"
     CDS             complement(35686..36078)
                     /gene="iraD"
                     /locus_tag="L2X49_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBN4796534.1"
                     /translation="MMRQSLQAVLPKISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(36075..36158)
                     /gene="idlP"
                     /locus_tag="L2X49_001424"
     CDS             complement(36075..36158)
                     /gene="idlP"
                     /locus_tag="L2X49_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBN4796535.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         8320 a         9654 c         9450 g         8965 t
ORIGIN      
        1 aaagaaaata tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt
       61 cccgtgccac cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc
      121 gccatgttgt tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg
      181 gtagcaaatt taacgattcg tgaagaattg ggattaagtg ccaccgaaat cggcgctttg
      241 ctctccgtgt tttcactcgc ttacgggatt gcgcaacttc cttgcggccc actattggat
      301 cgtaaaggcc cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggca
      361 atgtctggca tggtgcacaa ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt
      421 ggtgaagcgc cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa
      481 gagcgcggac gcccgatggg cttcttcaac gcagcttcta ccattggcgt tgccgtaagc
      541 ccaccgattc tggcggcgat gatgctggtg atgggctggc gcgggatgtt tattaccatt
      601 ggtgtactgg ggatttttct cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac
      661 gtagaactga ctgccgttga gcaagcttat ctcaatgcag gtagcgtcaa tgcccaccga
      721 gatccgctca gttttgccga atggcgcagc ctgttccgta accgtacaat gtggggaatg
      781 atgctcggat tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt
      841 tacctgcaaa cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct
      901 ttcctgtttg gggctgccgg gatgctggtc aacggttacg ttactgactg gctggtcaaa
      961 gggggaatgg ctccgattaa aagccgtaag atctgcatta ttgccgggat gttctgttct
     1021 gccgccttta cgctgatagt accacaagcg acaacatcca tgacggcggt tctgctgatt
     1081 ggcatggcac tgttctgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc
     1141 gcagttgctt ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc
     1201 tgcgcctctt ttgcgccgat cattactggt tttattgttg ataccaccca ctcattccgt
     1261 ctggcactaa tcatctgcgg ttgcgtcacc gcagcggggg cactggcgta catcttcctg
     1321 gttcgtcagc cgatcaacga cccacgtaaa gattaatctc cttatgcccg ataacatttt
     1381 gcttatcggg cattttcatg gcgattaaaa tgtttcccag ttctcctcgc tatctgccac
     1441 ggccatttta cgcggcgcag ctggcgtcac ggtttttacc acagccgatg tttcacgctg
     1501 ctgttgctga atccggaaca ctgccactgc ttcggtcagg cgactggcct gctcttccag
     1561 cgcggcggcg gcagcggcag actcttccac cagcgcggcg ttctgttgag ttacccggtc
     1621 catctcagca accgctaagc caacctgatc gataccacgg ctctgctcat cagaagcaga
     1681 agcaatttcg cccataatgt ccgtcacgcg ggtcacggcg ctgacaatct ccgccattgt
     1741 ttccccggcg ctttcgacca gcgtagagcc aacatccact ttccccaccg agtcttcaat
     1801 caggctttta atttcacgag ccgcctgggc gctgcgctgg gccagattac gcacttctcc
     1861 cgcgaccacc gcaaaaccgc gcccttgctc acccgcacgc gcagcctcaa ccgccgcgtt
     1921 caaagccaga atattggtct ggaaggcaat gccgtcaatt acgctgataa tatcggcgat
     1981 tttctgcgaa ctggtggaga tatcgcgcat agtctgcacc acgttatcta ccactttgcc
     2041 gccgcgttgc gccgtttcag aagcacttaa cgccagatgg ctggcctggc gcgcattctc
     2101 ggcgttctgt ttcaccgttg cggtcagttg ctccatgctg gctgccgtct cttccagcga
     2161 agcggcctgt tgctcggtgc gcgaagagag atcgttattg ccggtagcga tttcgctggc
     2221 accgctatag atggcattgg ccccgttgcg cacatcaccg acggtacgca tcagctctcc
     2281 ctgcatatgg cgcaaactct ctgccagttg ccccatctca ttagagccat ccacctcaat
     2341 cggtttcacc agatcgccgc ctgcaatatg acggatgctg tcaatcaggc gattcattgg
     2401 cgctaccagc gaggctttaa taccgaacca gacggcgaag atgaccgcca gtacgacgat
     2461 catcacgccc accagaatcc acatcgcctg gctgtaggag gcattgttat cgctgacggc
     2521 gatatcatag agccgatcgt tttgctccat gtaagccaca tactgcttct cgaaaccgtc
     2581 ttgatatccc tgggtcggct gatcaaagaa ctcgttgatt ttgcctgcac ctaacagttg
     2641 gatcagctcc gccagcgcat tgtgataaat atcgtaatta cgtttgatct ctgccgctgc
     2701 ggcggtgctc tgacgcgggt cacgcggcaa cgcttcgtaa tccgcccagt ttttttccgc
     2761 ctgtttcagc gaaatactgg cactctgcat cagctcagca acggttgaac cgctaccaat
     2821 attattctga tccatcatgt agcggatacc cgcgcggttg agggtgttac gcgtctgcaa
     2881 caacgcgacc cagctgccat tcagcgtgga ttgctgctgg cgaatggttt gtaaaacagt
     2941 gaaattttct ttgtcattct ttaaggcatt aaagaacaga ccgcctgatg tcagttgtaa
     3001 aaggccaaaa acggccaaaa ccagcagtaa gctggtcacg atcttgatac gttttaacat
     3061 ggtttctctt tcctgtggac cgatgcaaga ttttcggcct ggaaagcaaa aactttatga
     3121 aaaggtgtaa cccgccatcc atggaaaaac caggcgctgg ttatcagata ctaaaaagtt
     3181 attcattatc gcccgggatg gcttcttgtc gggaattgcc cagagcaata aattcctcta
     3241 acagatgggt taactgttgc attttctcgg cagtaaactg agcttcaatc tgccgatagg
     3301 cttcttcaat ctgtgtctga gcgcggttat acagcgcctg cccctctttg gttagcgaga
     3361 tatagagctt ccgctgatca ttgatcggct ttaatcgcaa cactaaaccg tcgcgttcca
     3421 tccgcgtgag gatcccggtc aggcttgggc gcaaaatgca ggcgcgatac gccagatcgt
     3481 gaaaatccat tgatgggctt tccgccagaa tacgcacgat gcgccactgt tgctcagtca
     3541 aattatgccg tttaacgatt gggcgaaaat aactcatcgc cgcttctcgc gcctggagca
     3601 acgcaatggt tagagagtcg tgcattatct ttcccctgaa aaattcaaaa tcattaatat
     3661 agaaacagtt aatcatcacg ttaccgggac tgcgctgccc ctgcaattct tcattttatc
     3721 aaaattgagc atgttttgtt aacttgctga tatttaatat tttattatct ttaaatgtaa
     3781 atagtttgtt aattagatca catttacatc acttactttt gccaaactac gcaatcggga
     3841 ataaaagtaa tcattaacat attaatgatt aagatgatcg aatccgagga gtggtaatga
     3901 aaggcactat cttcgccgta gcgttgaacc atcgcagcca acttgatgca tggcaggaag
     3961 cgttccagca atccccctac aaagccccgc ctaaaactgc ggtctggttt attaaaccgc
     4021 gcaatacggt gattggttgc ggtgaaccga ttccctttcc acagggtgaa aaggtactga
     4081 gcggtgcgac tgttgcgctg attgtgggaa aaacggcgac gaaagtacgt gaagaagatg
     4141 cggcagagta catcgccgga tatgcgctgg ctaacgacgt cagcctgccg gaagagagct
     4201 tttaccgccc ggcaatcaaa gcaaaatgtc gtgatggatt ctgccccatt ggcgaaaccg
     4261 tggctctcag caatgtcgat aatctgacca tctataccga gatcaacggg cgtcctgccg
     4321 atcactggaa taccgccgat ttacaacgta acgccgcgca gttgctgagc gccctgagcg
     4381 aatttgccac gctgaatcca ggcgacgcca ttctgctcgg cacgccacag gcgcgcgtgg
     4441 aaatacagcc aggcgatcgc gttcgtgttc tcgcagaagg tttcccgccg ctggaaaatc
     4501 cggtagtgga cgaacgtgaa gtgaccacgc gcaagagctt cccaacgcag ccacacccgc
     4561 acggcacgct gtttgccctc ggcctgaact acgccgacca cgccagcgaa ctggaattta
     4621 agccaccgga agaaccgctg gtgttcctga aagcgccaaa taccctcact ggcgataacc
     4681 agacctccgt gcgtccaaac aatattgaat acatgcacta tgaagcggag ctggtggtag
     4741 ttattggcaa acaggcgcgt aacgtcagcg aagccgatgc catggattat gtcgcgggct
     4801 acaccgtgtg taacgactac gccattcgcg actatctgga aaactactac cgccctaacc
     4861 tgcgggtcaa aagccgcgac ggactgacgc cgatgctttc aaccatcgtg ccgaaagagg
     4921 cgatcccgga cccgcataat ctgacccttc gcaccttcgt caacggcgag ttacgccagc
     4981 aaggcaccac cgccgatctg atcttcagcg tgcccttcct gatcgcctac ttaagcgaat
     5041 ttatgaccct gaatccgggc gacatgatcg ccaccggcac accaaaaggc ttatctgacg
     5101 tggtgcctgg cgatgaagtg gtggtggaag tagaaggcgt gggccgcctg gtgaaccgaa
     5161 ttgtgagtga ggaaacagcg aaatgaaaaa agtaaatcat tggatcaacg gtaaaaatgt
     5221 tgcaggtaac gactacttcc agactaccaa tccggcaacg ggtgaagtgc tggcggatgt
     5281 ggcctccggc ggtgaagcgg agatcaatca ggcggtagcg gcagcgaaag aggcgttccc
     5341 gaaatgggcc aatctgccga tgaaagagcg cgcgcgcctg atgcgccgtc tgggcgatct
     5401 gatcgaccag aacgtgccag agatcgccgc gatggaaacc gcggacaccg gcctgccgat
     5461 ccatcagacc aaaaatgtgt tgatcccacg cgcttcccac aactttgaat ttttcgcgga
     5521 agtctgccag cagatgaacg gcaagaccta tccggttgac gacaagatgc tcaactacac
     5581 gctggtgcag ccggtgggcg tttgtgcgct ggtatcgccg tggaacgtac cgtttatgac
     5641 cgccacatgg aaggtcgcgc cgtgtctggc gctgggcaat accgcggtac tgaaaatgtc
     5701 ggaactctcc ccgctgaccg ctgaccgcct gggtgagctg gcgctggaag ccggtattcc
     5761 ggcaggcgtg ctgaacgtgg tacagggcta cggcgcaacc gcaggggatg cgctggttcg
     5821 tcatcatgac gtacgtgccg tgtcgttcac cggcggtacg gccaccgggc gcaacatcat
     5881 gaaaaacgcc gggctgaaaa aatactccat ggaactgggc ggtaaatcgc cggtgctgat
     5941 ttttgaagat gccgatattg aacgcgcgct ggacgccgcc ctgttcacca tcttctcgat
     6001 caacggcgaa cgctgcaccg ccggttcgcg catctttatt cagcaaagca tctacccgga
     6061 attcgttaaa cgctttgccg aacgcgccaa ccgtctgcgc gtgggcgatc cgaacgatcc
     6121 gaatacccag gttggcgcgc ttattagcca gcagcactgg gaaaaagtct ccggctatat
     6181 ccgtctcggc attgaagaag gcgcaaccct gctggcgggc ggcccggata aaccgtccga
     6241 cctgcctgcg cacctgaaag gcggcaactt cctgcgccca accgtgctgg cagacgttga
     6301 taaccgtatg cgagtcgccc aggaagagat tttcgggccg gtcgcctgcc tgctgccgtt
     6361 taaagacgaa gccgaaggct tacgtctggc aaacgacgtg gagtacggcc tcgcgtcgta
     6421 catctggaca caggatgtca gcaaagtgtt acgcctggcg cgtggcattg aagctggcat
     6481 ggtgttcgtc aacacccaga acgtgcgtga cctgcgccag ccatttggcg gcgtaaaagc
     6541 ctccggcacc gggcgtgaag gcggtgagta cagcttcgaa gtgttcgcgg aaatgaagaa
     6601 cgtctgcatt tccatgggcg accatccaat tccgaaatgg ggagtctgat atgggtaagt
     6661 tagcgttagc cgccaagatc acgcacgtac cgtcgatgta tctctctgaa ctgccaggga
     6721 aaaaccacgg ttgccgccag ggcgcgatcg acgggcataa agagatcagc aagcgttgcc
     6781 gggaaatggg cgtcgatacc attatcgttt tcgataccca ctggctggtc aacagtgctt
     6841 atcacatcaa ctgtgcagac cattttgaag gcgtctacac cagcaacgag ctaccgcatt
     6901 ttatccgcga tatgacctac aactacgagg gcaacccgga gttggggcag cttattgccg
     6961 atgaagcctt aaagctcggc gtgcgggcaa aagcgcacaa cattcccagc ctgaaactgg
     7021 aatacggcac gctggtgcca atgcgctaca tgaatgaaga caagcacttc aaagtggttt
     7081 ccatttcagc tttctgcact gttcacgatt ttgccgacag ccgcaagctg ggcgaggcga
     7141 ttctgaaagc gatcgaacag tacgacggca ccgtggcggt ccttgccagc ggttcgttat
     7201 cgcaccgctt tattgacgat cagcgagcgg aagaagggat gaacagctac acccgcgagt
     7261 tcgaccgcca gatggacgag cgcgtggtga agctgtggcg cgaaggccag ttcaaagagt
     7321 tctgcaatat gctgccggag tacgccgact actgctacgg cgaaggcaat atgcacgaca
     7381 cggtgatgct gctggggatg ctcggctggg ataaatacga cggcaaggtg gagtttatca
     7441 ccgagctatt cccaagctct ggcaccggtc aggttaacgc tgttttcccg cttcccgcgt
     7501 aaggacgttt tatgccgcac tttatcgttg aatgcagtga caacatccgc gaagaagccg
     7561 acctgccggg gttgttcgcc aaagtgaatc cgacgctggc agccacgggt atttttccgc
     7621 tggcgggtat tcgcagccgt gtgcattggg tcgatacctg gcagatggcc gacgggcagc
     7681 gtgattatgc cttcgtgcat atgacgttga aaatcggcgc aggtcgcagc ctggaaagcc
     7741 gtcagcaggc gggtgaaatg ctgtttgaac tgattaaaac gcacttcgcc gccctgatgg
     7801 agagccgcct gctggcgttg tcgtttgaga ttgaagagct gcatccgacg ctgaatttta
     7861 aacaaaacaa cgtgcacgca ttgtttaagt agcgcgcaga ttgcccggtg gcgctgcgct
     7921 taccgggcct acaaaacccc aaaccgtaca ccgtaggccg gataaggcgc agccgcatcc
     7981 ggcaatgcca caggatatcg ctatgttcga taaacatacc cacaccctga tcgcccagcg
     8041 tctggatcag gcagaaaaac agcgcgaaca gatccgcgcg atctcactgg attacccgga
     8101 gatcaccatc gaagacgctt acgcggtgca gcgcgaatgg gttcgcctga aaatcgccga
     8161 aggtcgcacg ctgaaaggcc acaaaatcgg cctgacctcg aaagcgatgc aggcaagctc
     8221 gcagatcagc gaaccggatt acggcacgct gctggatgac atgttcttcc acgatggcag
     8281 cgatatcccg accgatcgct ttatcgtgcc gcgcattgaa gtggagctgg ctttcgtgct
     8341 ggcaaaaccg ctgcgtgggc caaactgcac gctgttcgac gtttacaacg ccacggacta
     8401 tgtgatcccg gcgctggagc tgatcgacgc tcgctgccac aacatcgatc cggaaaccca
     8461 gcgcccgcgc aaagtgttcg acaccatttc tgataacgcc gccaacgccg gagtgatcct
     8521 cggcggtcgt ccgatcaaac cagacgaact ggatctgcgt tggatctccg ccctgatgta
     8581 tcgcaatggc gtgattgaag aaaccggcgt cgccgctggt gtgctgaatc atccggcaaa
     8641 cggcgtggcc tggctggcga acaaactcgc accctatgac gtacaactgg aagccgggca
     8701 aatcattctc ggtggttcgt tcacccgccc ggttccggcg cgtaagggcg acaccttcca
     8761 cgtcgattac ggcaacatgg gttccattag ctgccgcttt gtttaaggag agaacgatgg
     8821 aaaacagttt taaagcggcg ctgaaagcag gccgcccgca gatcggatta tggctggggc
     8881 tgagcagcag ctacagcgcg gagttactgg ccggagcagg attcgactgg ttattgatcg
     8941 acggtgagca cgcgccgaat aacgtgcaaa ccgtgctcac ccagctacag gcgattgcgc
     9001 cctaccccag ccagccggta gtacgtccgt cgtggaacga tccggtgcaa atcaaacaac
     9061 tgctggacgt cggcacacaa accttgctgg tgccgatggt acaaaacgct gacgaagccc
     9121 gtgaagcggt acgcgccacc cgttatcccc ccgccggtat tcgcggtgtg ggcagtgcgc
     9181 tggcccgagc ctcgcgctgg aatcgcattc ctgattacct gcaaaaagcc aacgatcaaa
     9241 tgtgcgtgct ggtgcagatc gaaacgcgtg aggcaatgaa gaacttactg cagattctgg
     9301 acgtggaagg ggtcgacggc gtgtttatcg gcccggcgga tctgagcgcc gatatgggtt
     9361 atgccggtaa tccgcagcac ccggaagtac aggccgccat tgagcaggcg atcgtgcaga
     9421 tccgtgaatc gggcaaagcg ccggggatcc tgatcgccaa tgagcaactg gcaaaacgct
     9481 atctggaact gggcgcgctg tttgtcgccg tcggcgttga caccaccctg ctcgcccgcg
     9541 ccgctgaagc gctggcagca cggtttggtg cgcaggccac cgccgtgaag cccggcgtgt
     9601 attaatgccg gatgcgctgc gcttatccgg cctacactcg caccaaaccg taggccggat
     9661 aaggcgttta cgccgcatcc ggcaaaaagc cgtaccaaat cgcggaccgg ataaggcgtt
     9721 tgtgcgcatc cgccataaac cttgtcgtac cctacaaaaa tcccattaga ggaagaaaaa
     9781 tgagcgacac ctcacctgcc ataccggaga gtatcgatcc ggcgaatcag cataaagcgc
     9841 tgactgccgg acaacaggcg gttattaaga agctatttcg ccgcctgatc gtctttctgt
     9901 tcgtgctgtt tatcttctcg ttccttgatc gcatcaacat cggctttgcc ggactcacga
     9961 tgggacgcga cctcggtctg agcgccacca tgtttggcct cgctaccacc ctgttctatg
    10021 ccgcttatgt catcttcggc attcccagca acattatgct gagtattgtc ggtgcgcggc
    10081 gctggatcgc caccatcatg gtgctctggg gcatcgcctc tactgccacc atgtttgcca
    10141 ctggccccac cagcttgtac gtactgcgta tactggttgg cattaccgaa gccggctttc
    10201 tgcctggtat tctgctgtat ttaaccttct ggtttccggc ctatttccgc gcccgtgcca
    10261 acgccttgtt tatggtagca atgccggtaa cgacagcgtt gggctcgatc gtttccggct
    10321 acattttgtc gctggatggc gtcatggcat taaaaggctg gcagtggctg tttttgctgg
    10381 aaggcttccc gtcggtatta ctcggcgtca tggtgtggtt ctggcttgat gactcaccgg
    10441 acaaagctaa gtggctgacg aaagaagaca aaaaatgcct gcaagagatg atggataacg
    10501 atcgtctgac gctggttcag ccagagggag ccatcagcca ccacgccatg caacaacgca
    10561 gtatgtggcg ggagatcttc actccggtgg tgatgatgta taccctggcg tatttctgcc
    10621 tgaccaacac acttagtgcg atcagcatct ggacaccgca gatcctgcaa agttttaatc
    10681 agggcagcag taatatcacc atcggcctgc tggccgccgt accgcagatt tgtaccattc
    10741 tcgggatggt ctactggagc cgtcactcag atcgccgcca ggaacgaagg catcacaccg
    10801 cccttcctta tttgttcgct gccgcaggtt ggttactggc ttcggcaact gatcacaaca
    10861 tgatccagat gctggggatc attatggctt cgaccggatc attcagcgca atggcgattt
    10921 tctggacaac accggatcag tccatcagcc tgcgggcacg agcgatcggt attgcggtga
    10981 tcaacgccac tggcaacatt ggttcagcgt taagtccgtt tatgatcggc tggttgaaag
    11041 atttgaccgg cagctttaac agtggattgt ggtttgttgc cgcgctgctg gtgattggtg
    11101 cggggattat ctgggcgatt ccaatgcagt cctcccgtcc gcgagcgacc ccgtaaggaa
    11161 cgacgatgtg tgaccgtcag attgccaata ttgatatcag caaagagtac gatgaaagcc
    11221 tgggcacgga cgatgtgcat tatcagtcgt tcgcccgcat ggcggcattt tttggccgcc
    11281 atatgctgcc acatcgccac gaacagtact ttcagatgca tttcctcaat agcggacaga
    11341 ttgagctaca acttgacgat catcgctact cggtggaagc gcccctgttt gtcctgacgc
    11401 cgccgtcagt acctcatgcg tttattacgg agtctgacgc cgacggtcat gtgttgacgg
    11461 tacgggaaga tctgatctgg cccctgctgg aagttcttta tccgggcact cgggaaacct
    11521 tcggcctgcc ggggatttgc ctgtcactgg cagataaacc cgacgaactg gcggcgctgg
    11581 aacactattg gcaactgata gagcgggaat cggtagaaca actgcctgga cgggaacaca
    11641 ccctgacgtt actggcacag gcagtattca ccctactgct gcgtaatgca aaactcgacg
    11701 accatgccgc cagcggaatg cgcggagaat taaaactgtt ccagcgtttt aatatgctga
    11761 ttgaaagcca ttttcatcag cactggacag taccggatta cgctaacgaa ctgcatatca
    11821 ccgaatcacg cctcacggac atctgccgcc gctttgccaa ccgtccacca aaacggttga
    11881 ttttcgacag gcagctgcga gaagccaaac ggctgctgct gttttctgat aacgcagtga
    11941 acaatattgc ctggcaactc ggttttaagg atccagctta ttttgcgcgc ttttttaatc
    12001 gcttagtcgg ttgctcgccc agtgcttatc gtgccaaaaa agtacctgtg acgtgaaaat
    12061 cccttagtct taacgggaaa ccaggcacca cctgctattc ccctttctcc gcctggagca
    12121 aggggattta gtaacgtcaa aaatcgctaa aagcgacctc gatcacaaaa ccgtacccga
    12181 cctgaaaagt accagccatt gtcccaaaag tgtcttcccg taatcgcccc tgcaagcttc
    12241 aattgaataa caaaaacaac acacaaaatt aacaatcgta ttccgattaa tactgtagag
    12301 gtcgacatga aaccagaaga tttccgcgcc agtacccaac gtccgttcac cggggaagag
    12361 tatctgaaaa gcctgcagga tggtcgcgag atctatatct atggcgagcg agtgaaagac
    12421 gtcaccactc atccggcatt tcgtaatgcg gcagcgtctg ttgcccagct gtacgacgca
    12481 ctgcacaaac cggagatgca ggactctctg tgctggaaca ccgacaccgg cagcggcggc
    12541 tatacccata aattcttccg cgtggcgaaa agtgccgacg acctgcgcca gcaacgcgac
    12601 gccatcgctg agtggtcacg cctgagctat ggctggatgg gccgtacccc agactacaaa
    12661 gctgctttcg gttgcgcact gggcgcgaat ccgggctttt acggtcagtt cgagcagaac
    12721 gcccgtaact ggtacacccg tattcaggaa actggcctct actttaacca cgcgattgtt
    12781 aacccaccga tcgatcgtca tttgccgacc gataaagtaa aagacgttta catcaagctg
    12841 gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga aagtggttgc caccaactcg
    12901 gcgctgactc actacaacat gattggcttc ggctcggcac aagtaatggg cgaaaacccg
    12961 gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg gcgtcaaatt aatctcccgc
    13021 gcctcttatg agatggtcgc gggtgctacc ggctcaccgt atgactaccc gctctccagc
    13081 cgcttcgatg agaacgatgc gattctggtg atggataacg tgctgatccc atgggaaaac
    13141 gtgctgatct accgcgattt tgatcgctgc cgtcgctgga cgatggaagg cggtttcgcc
    13201 cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga aactcgactt cattacggca
    13261 ctgctgaaaa aatcactcga atgtaccggc accctggagt tccgtggtgt gcaggccgat
    13321 ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat tgagtgactc gatgtgttct
    13381 gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc atgccgcact gcaaacctat
    13441 cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca ttatcgaacg caacgttacc
    13501 agtggcctga tctatctccc ttccagtgcc cgtgacctga acaatccgca gatcgaccag
    13561 tatctggcga agtatgtgcg cggttcgaac ggtatggatc acgtccagcg catcaagatc
    13621 ctcaaactga tgtgggatgc cattggcagc gagtttggtg gtcgtcacga actgtatgaa
    13681 atcaactact ccggtagcca ggatgagatt cgcctgcagt gtctgcgcca ggcacaaagc
    13741 tccggcaata tggacaagat gatggcgatg gttgatcgct gcctgtcgga atacgaccag
    13801 aacggctgga ctgtgccgca cctgcacaac aacgacgata tcaacatgct ggataagctg
    13861 ctgaaataac gcagcaggag gttaagatgc aattagatga acaacgcctg cgctttcgtg
    13921 acgcaatggc cagcctgtcg gcagcggtaa atattatcac caccgagggc gacgccggac
    13981 aatgcggaat tacggcaacg gccgtctgct cggtcacgga tacaccaccg tcgctgatgg
    14041 tgtgcattaa cgccaacagt gcgatgaacc cggtttttca gggcaacggt aagttgtgcg
    14101 tcaacgtcct caaccatgag caggaactga tggcacgcca cttcgcgggc atgacaggca
    14161 tggcgatgga agagcgtttt agcctctcat gctggcaaaa aggtccgctg gcgcagccgg
    14221 tgctgaaagg ttcgctggcc agtcttgaag gagagatccg cgatgtgcag gcgattggca
    14281 cacatctggt gtatctggtg gagattaaaa acatcatcct cagtgcagaa ggtcacggac
    14341 ttatttactt taaacgccgt ttccatccgg tgatgctgga aatggaagct gcgatttaag
    14401 ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat tgtgatctca atcacatatc
    14461 cacccgatga ataattcttg cccaaccgct caccgccatt cctgaccgct tacatcccta
    14521 aaataaccac tcagttattt accttacttt acgcgcgcgt atctctggca acatcactgc
    14581 aggatagcgg tcaatttacc tcctcaaacg caacgcaaac ctagaacggc ttcggcctat
    14641 gattaacaaa ttactctatg gattttggtt tgcaggaagg cagcaagtga gtgaatcccc
    14701 gggagcttgc aacagtaagt gacagaggtg aacgaacgca gctgccgcac ctgtaagcca
    14761 aaagacgacg agtaaaatgc caggttttac tatggatacg aaaaaactat tcaagcacat
    14821 accctgggtg attctcggaa tcatcggtgc attctgcctc gcggtagttg cattacgtcg
    14881 gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct gtgtcggtgt atctggtggc
    14941 ttatcgctac tacagtctgt acatcgccca gaaggtgatg aaactcgacc ccacgcgcgc
    15001 gacgcctgcg gttattaaca acgacggtct gaactatgtt ccgaccaacc gttacgtgtt
    15061 gtttggtcac cacttcgccg ctatcgccgg tgctggtccg ctggttggtc cggttctcgc
    15121 cgcgcagatg ggctacctgc ccggcacgct gtggctgctg gcgggggtag tgctggccgg
    15181 tgcggtacag gactttatgg tgctgtttat ctcctctcgc cgtaacggcg catctctcgg
    15241 tgagatgatc aaagaagaga tggggccagt accgggaact atcgcgctgt ttggctgttt
    15301 cttaatcatg atcatcatcc tcgccgtcct ggcgctgatt gtggttaaag ccctggccga
    15361 aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg attgcgctgt ttatgggtat
    15421 ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc tctgttattg gtatcgtgct
    15481 gctggttgcc tctatctact tcggtggcgt gattgctcac gacccgtact ggggtccggc
    15541 actgaccttt aaagacacca ccattacctt cgcgctgatt ggctatgcgt ttatttccgc
    15601 gctgctgccg gtgtggctga tcctcgcacc acgcgactat ctggcaacct tcctgaaaat
    15661 cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg aacccggaac tgaaaatgcc
    15721 tgcgatgacc cagtacattg acggtactgg cccgctgtgg aaaggcgctc tgttcccgtt
    15781 cctgttcatc accatcgcct gtggtgcggt atctggcttc cacgcgctga tctcttccgg
    15841 tacgacgccg aaactgctgg ctaacgaaac cgacgcgcgt ttcattggct acggcgcaat
    15901 gctgatggag tccttcgtgg cgattatggc gctggttgct gcgtccatca tcgaaccggg
    15961 tctttacttc gcgatgaata ccccgcctgc tggccttggc atcaccatgc ctaacctgca
    16021 tgaaatgggt ggcgagaacg cgccgatcat catggcgcag ttgaaagacg ttaccgcaca
    16081 cgcggcagcg accgtcagct cctggggctt cgtgatttcg ccagagcaga tcctgcaaac
    16141 cgcgaaagac attggtgagc cttctgtcct gaaccgtgca ggtggtgcgc cgacactggc
    16201 ggtaggtatc gcgcacgtgt tccacaaagt gctgccgatg gctgacatgg gcttctggta
    16261 tcacttcggt attctgttcg aagccctgtt catcctgacc gcgctggatg cgggtacccg
    16321 ttctggccgc tttatgctgc aggacctgct gggtaacttc atcccgttcc tgaaaaaaac
    16381 cgactctctg gttgctggta tcatcggtac tgcgggctgt gtgggtctgt ggggctacct
    16441 gctgtatcag ggcgtggtcg atccgctggg cggcgttaag agcctgtggc cgctgttcgg
    16501 tatttccaac cagatgctgg cagccgtagc gctggtactg ggcaccgttg tgctgattaa
    16561 gatgaagcgc acccaataca tctgggtaac tgttgttccg gctgtatggc tgcttatctg
    16621 caccacctgg gcgctgggcc tgaaactgtt cagcaccaac ccgcagatgg aaggcttctt
    16681 ctacatggca agccagtaca aagagaagat tgctaacggt actgacctga cggcgcagca
    16741 gattgccaac atgaaccaca tcgttgtgaa caactacacc aacgcaggcc tgagtattct
    16801 gttcctgatt gtggtgtaca gcatcatctt ctacggtttc aaaacctggc ttgcggtgcg
    16861 taacagcgac aaacgtactg acaaagaaac accgtacgtt ccaatcccgg aaggcggcgt
    16921 gaagatctct tcgcaccact aaccgtgttt agccccgctt cggcggggct ttgttcttat
    16981 cagagtgaac tatgtttggt aacttaggac aggcaaaaaa atatctcggc caggcggcga
    17041 agatgttgat tggcattcca gactacgaca actacgtcga gcatatgaag accaaccatc
    17101 ccgacaagcc gtacatgagc tatgaagaat tcttccgcga acgccagaac gcgcgctacg
    17161 gcggcgatgg taaaggcggt atgcgctgtt gttaatggag agagcatgaa cccgattgca
    17221 gttaccctac tcaccggttt tttaggcgcg ggcaaaacca ccctgctgcg ccatattctt
    17281 aacgaacaac acggctacaa gattgccgtg attgaaaacg aattcggcga agtctccgtt
    17341 gatgatcaac tgattggcga ccgcgccacg cagatcaaaa cgctgaccaa cggctgcatc
    17401 tgctgttcgc gctccaacga gctggaagac gcactactcg acctgctgga taatctcgac
    17461 aagggcaata ttcagttcga tcgtctggtc attgaatgca ccggcatggc cgatcccggc
    17521 ccgattattc agaccttttt ctcccatgag attttatgcc agcgttacct gctggacggc
    17581 gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga tgaaccagtt caccatcgcc
    17641 caatcccagg ttggctatgc cgaccgtatt ctgctgacta aaaccgacgt cgcaggcgaa
    17701 gcagaaaaac tgcgtgaacg tctggcgcgc atcaacgccc gcgcaccggt ctacaccgtc
    17761 acccacggcg acatcgacct gggtctgctg ttcaacacca acggttttat gctggaagaa
    17821 aacgtcgtca gcaccaaacc gcgtttccat tttatcgcgg acaaacaaaa cgatatttcg
    17881 tcgattgtgg tggaactgga ttacccggta gatatcagcg aagtttcccg ggtgatggaa
    17941 aacctgctgc tggaatcggc ggataaactg ctgcgttaca aagggatgct gtggattgac
    18001 ggcgaaccta accgcctgct gttccagggc gtccagcgcc tctacagcgc cgactgggac
    18061 cgcccgtggg gcgatgaaaa accgcatagc acgatggtgt ttatcgggat tcagttgccg
    18121 gaggatgaga ttcggtcagc gtttgcggga ctcaaaaagt aaccagccct ccaacatcac
    18181 aatacctgag actttatcac cctcatattc ctccagaata tgagggcatt cgggcttaat
    18241 tatctctgaa cgtgcaacac ttcctgcgac tccttatgcg cctccacatc ctgcttcgtt
    18301 aaccagagcg acttacgacc aaaattttcg tacattttca gctgatcttc atagtgctta
    18361 tcaactgttc catcgggagc aataaaccca ctctgaccgg gtgcgaccac atcccaggca
    18421 agcaccggac gatcgcttgt cgttggtaag aaaacaatca tatcgttttc tgttccacgg
    18481 ttttgatact ccgcctgatg acgcgtttct tccactgcgg cctgcggtac accaaagaaa
    18541 ttatttgccc ggaacgttaa ggccattgca ggtgttttcc agttactcac attattgcca
    18601 tagcgtttgg aaagagtctc ccaggtatct tccagcgcag ccaacacaac ctcctgctgt
    18661 ggtttcccag caaacagatc aaccgcctgt gggattggtg atttgtctcc ctgcaccgcc
    18721 tcatacaaaa tttttgctcc aacacttata ttcagcgaac cagttgggcc gtcctgggtt
    18781 gtttcgtagc cactggcgct gtaccactta tcaaatggca taggtacggc agccactacg
    18841 gtacgcttca acatactggt cagccaaacg ttcaggatgg cagagcctgg ctgctgccag
    18901 gttttaccat cataattaag caaattgatg ccatcccaac gtgttaatgt atccaccaac
    18961 tgacgacgcg gatcgctctg tgtcaaacca gatgtcgctg cttgcagagt aggtaaaaaa
    19021 agcctcaggt taagatcctt acgactggtt tggcgaataa catcccatgc ctgatcagca
    19081 gttaagcgtg gcttttgctc aagcagtcgg tcgatctccg taacgcgatc tgcccccccc
    19141 ccacaaaaag gcaaacagat ccgaagcggg ataatctttt tggggagaat tgttccagtt
    19201 agcaatatat cccgacaggg ggttatacac cttagggttc atttcaaaag gcaatagccc
    19261 tttccagtcc cattttcccg taccaggaac gggtaattgc ggatcatggc ctgattgacg
    19321 atctggataa gcaccagtat gaacataacc aatattgccg tttacatcag catagtacca
    19381 gttgatggtc agtgcttgtt tcgctgcctg ctgtgtccac tcctgccaat ttttggcctt
    19441 catctgatga gtccaggcca gcaaagacgc cacctcttta ccatcccatg cgcgggattt
    19501 agcgtaagcc gtttgtgtcg tctggtcagt ttggagaatg ttgccatgca ccgtacgcca
    19561 gacagtaaag gtctctgcct gaccattttt caccgtaatg gtttcctcac ggcttaacat
    19621 tttcacccac ttaccattat gcaagtagta gcctggtttc tctgccgaca gccgttcagc
    19681 aaaaatatcg acatcatcgc cgaaacctgc cgttgatccc caggaaatca caccattatg
    19741 accaaaaacc agcccaggat aggcaaatgg tgtattgcca gtgacatcat aaccagcacc
    19801 gtgcagacca ataccataag tatacgcagg cgcataccag ccaaactgcg gaccatttac
    19861 catgattgct ttcgcatcct gggctttgct tttgccgatc acccacatat tgctggtcgt
    19921 tggatacccc gccagaccat tggcaccacc ctgtgcaaat tgtgcagcaa tagtttcccg
    19981 gttcttccct gctgttaacg ccagcagtgc gccatccgcc ccttttgctg gtcggtcaag
    20041 cattggtgca ggtaaatcgt agcgtggcaa cagagctgct gtttgcgagt tttgctgatt
    20101 aaatttaagt gggtagttac tctcttgtac ggcaatagtg gttggcgctg atgggtttac
    20161 cagccatttc aactgattaa ataccgccat gccttgtgat acaccatatt tatcttttaa
    20221 agccgttagc agtgccagat tatcaatttc gctagtgcta tcagagaagc ggtttgccat
    20281 ggtgcccaca aatatcatcg cgacatcaaa cggttcccag cgcttaggag taaagccaaa
    20341 tgtattaaac tgttttggta agagcgtctc tggattggta tttaccttat caatccaggc
    20401 attcattcca tcagcgtagc cttgcagaat ggacatatcc tctggggaaa gggcagcaat
    20461 ttgcgcccgg atagcatccg gccagtagtt acgacggata tctttatcaa atttcacaaa
    20521 atctttgcca agcacttccg cgacagtccc ttgagtactg cgacgtgcca tttccatctg
    20581 aaaaaggcga tcttgtgcta ctacatagcc atagccataa aataggtgcc atgtatcatt
    20641 ggcataaata tgcggcatgc cgtattcatc gcgaacaatc tttatctcac ttgacgactg
    20701 ctcagccagt gcaggtaagc tccaataata catcagggaa gcagtaacac agttcacgat
    20761 catacgattt ctatttttca ttgtatcctc tggcaggtgt ataattagcg aattgatact
    20821 agcaacgaag cttcggttat ctatttgcag agaattgttt cattatgaac tgtgagcgcg
    20881 ataactaatt tttgatacaa aaatttcatt aatgtgatct aaaacgaatc gctttattca
    20941 ccgctgatga ttttgttcta ttcctttttg tgacagatcc cccggacgca tagatgatga
    21001 cccccagaga gacagctcag gtcattatct gtatgtaaat cagaaaaata tgttgtaaac
    21061 gacttttatg gttggatttt gtagcaattt tgaatgcaga caggcagcat ttgccaacac
    21121 gccgcccata gttcactcca catcctccct gcgtaaccca atatccctca accgttcatc
    21181 actcatctgc tgtaacactc gtcgggtctg catttgccgc cgccaacgcc tcaccgcatg
    21241 ccagagttgc acaagtctga taaacggcgt tttggctttg ttttcgtgaa attccatctg
    21301 ctgcctcctc atctttcaga ggcttcatca tccgccttct ggcagttcac aatacagatg
    21361 caaatattag ttttatttaa catacagaca ggttaaaaca gcatctgcat ccggtttttt
    21421 agcctcatct gtaccggttt ttctttctgt atgggcccaa taaggaatac agcatgacgc
    21481 gttatcaaca tctggcgact ctacttgccg agcggattga gcaagggctg tatcgtcacg
    21541 gggagaaatt gccgtcggtg cgcagcttaa gtcaggagca cggcgtcagc atcagcaccg
    21601 tgcaacaggc gtatcagacg ctggaaacga tgaggctcat cactccgcag ccgcgttcgg
    21661 gttattttgt cgcacaacgt aaagcccagc cgcctgtacc gccgatgacg cgtccggtgc
    21721 agcgcccggt ggaaattacc cagtgggatc aggtgctgga tatgctggag gcgcatagcg
    21781 acaattccat tgttccgtta agcaaaagca cgccggatgt cgaaacgccc agcctgaaac
    21841 cgctgtggcg tgagctaagc cgggtggtgc agcataatct gcaaaccgtt ctcggttatg
    21901 acttgttagc cggtcagcga gtgttgcggg agcagattgc ccgcctgatg ctcgacagcg
    21961 gctcggtggt caccgccgat gacatcatca tcaccagcgg ctgccataac tcgatgtcgc
    22021 tggcgttaat ggcggtgtgt aaaccgggcg atattgtcgc ggtcgaatct ccctgttatt
    22081 acggttcgat gcagatgctg cgaggcatgg gcgtgaaagt gattgaaatc ccaaccgatc
    22141 cagaaactgg catcagcgtt gaagcactgg aactggcgct ggaacagtgg ccgattaaag
    22201 gcatcattct ggtgccaaac tgtaataatc cgctgggatt tattatgccg gacgcacgca
    22261 aacgggccgt tctctctctc gctcagcgtc atgatattgt gatttttgaa gatgatgtct
    22321 acggcgaact ggcaacggag tatccgcgcc cgcggaccat ccattcatgg gatatcgacg
    22381 ggcgagtgct gttgtgcagc tcgttcagta aaagtattgc tccaggcctg cgcgtgggtt
    22441 gggtcgcacc ggggcgttat cacgataaac tgatgcatat gaaatacgcc atcagcagct
    22501 ttaatgtgcc gtccacgcaa atggcggcgg caacgtttgt gttggaaggc cactatcatc
    22561 gccatatccg gcggatgcgg cagacttatc agcgcaatct ggcgctttat acctgctgga
    22621 tacgggaata ttttccctgc gaaatctgta ttacgcgccc gaaaggcgga tttttactgt
    22681 ggatagaatt gcctgaacag gtcgatatgg tctgcgttgc gcggcagcta tactgcatga
    22741 aaatccaggt ggcggcaggc tcgattttct cggcttccgg caaataccgt aattgtctgc
    22801 gcatcaactg cgctttgccg ctcagcgaaa cctatcgcga agcactaaag caaattggcg
    22861 atgccgtgta tcgggcaatg gaataaattc gaattagtgc cgaggcccct gatttactct
    22921 cacgaataac ctgtgactcg ttttatcttt atagcgacat cgccacgttc cggcatggct
    22981 taggcaaagt gtgcttcgtt catgccagat gccatgtgaa cgccttacca ggtctacaaa
    23041 atattaaaag ttcaatatat ttaatggatt atgtagacct gataagcgta gcgcatcagg
    23101 ctgctttgcg tttgtcatca gcctctgatt agtactcgat ccatggttcg cgcttgcttt
    23161 caggcaaggg ctgatctaat gcctgcattt gctctgccga aagtccggta tatcgttgta
    23221 tccattcagg atcggcccca ttctgcaaca acaatcggag gaggctccgt tccccttcta
    23281 ctttcccttc tttcctcccc ctctcccttc ccaacagcca tccatcatta tgaattcgct
    23341 ctgcaattgt cattattcgc tccctgtggt gtggcattcg cctggtaagt tcgctgataa
    23401 acgccttaaa acgcgcttca tcgccagtca gtaaaatgta atttaacagc gcagttatct
    23461 ggctgtcatt agcacactct gtaaccagta atactaccag ttggtcaata agccccatca
    23521 gatcgcgctg gcgaatatgc ttttggatca actccaacag ggcgactctg cgatgctgca
    23581 caatctcgtc gtctggcacg acagtgacat ccaccagcgg gaacgctgcg ctataaagtt
    23641 tccgtgcggt agtcggggcg gcaaattcgt ccagccagca cagggaccag gggtaaggac
    23701 tacggctacc gtgataaaac agcatcggga taaccagcgg tagcggccgg cgtttatcat
    23761 gctcgatatg gcgctgcatc accgccatgg aatatcgcat caggcgaaac gccatatgaa
    23821 tatcctcgcg gctctgatgt tcaatcacta cgtaaatata accatcacct tcacgggtct
    23881 ttaccgacca cagaatatcg gagtgtagcg cccgcaattt ttcatcgaca aagctggcgg
    23941 attccagttt taagctgtcg agatcgcaca gttcacgtaa atctttaggt aagtggatct
    24001 ccataaaatc ccgcgcggtg ccagggtgcg tgagaaagga cttaaataat gcgtcatgcg
    24061 gcgtgctggt cgagaagttt gtcatggcga tccgtcacct gtagataaga tggcccgact
    24121 ttagcggcta acaaaaccat tctcacccgg caatttcccg ttccccgagg cgctttccag
    24181 gctattttta ttcttctgca aacgatacat tttttcggaa acaggctcgc gttttacatt
    24241 tcctgtaccc ggcagatatt cccctttcca ccgcgctctt ttgattaacc gcaaatttta
    24301 attacactta aggtgtatat tttctatgca acccatcaat taaagaggtg taatgtgctg
    24361 atgactattt gtaatcgtta tacattctga cctggagtca gaaggtattt ctctgtctgt
    24421 gtgttcacag gcagtgtggt tgattacatg aaattcagta catttgtcct tctcgcctgc
    24481 ctttcgtcat taccgacggt attgaatttc gttttccccg ttggggttct ccggacaagg
    24541 agttgtttgt tatgccacgt ttttttgccc gccatgccgc cacgctgttt ttcccgatgg
    24601 cgttgatttt gtatgacttt gccgcgtacc tgtcgacgga tctgatccag ccggggatca
    24661 ttaatgtggt ccgtgatttt aatgccgatg tcagtctggc cccggcagcc gtcagtctct
    24721 atctcgcggg cggtatggcg ttacagtggc tgctggggcc gctttccgac aggattggcc
    24781 gcaggccggt gctgattacc ggggcgctaa tatttaccct tgcctgcgct gcgacaatgt
    24841 tcacaacgtc tatgacacag tttcttatcg cgcgtgcaat tcagggcacc agtatctgtt
    24901 ttattgccac cgttggttat gtcacggtgc aggaggcgtt cggccagaca aaagggatca
    24961 agttgatggc gattatcacc tccatcgtac tgattgcgcc gattattggc ccgctttccg
    25021 gcgcagctct gatgcacttt gtgcactgga aagtcctttt tgccatcatt gcagttatgg
    25081 gttttatctc atttgttggc ttactgctgg cgatgccaga gacggtgaag cgcggcgcgg
    25141 ttccatttag cgccaaaagc gtctttcgcg attttcgtaa tgtcttttgc aatcggctgt
    25201 tcctctttgg cgcagcaacc atctctttaa gctatatccc aatgatgagt tgggtggctg
    25261 tctcgccggt gatcctgatc gacgcgggcg gcttaacaac ttcgcagttc gcctggacac
    25321 aggttccggt gttcggcgcg gtgattgtcg cgaatgccat cgtggcgcgt ttcgttaaag
    25381 atccgaccga accgcgcttt atctggcgtg ctgtacccat tcaactggtc ggcctcgcgc
    25441 tgttgattat cggcaatctg ctgtcaccgc acgtctggct gtggtcggtg ctgggcacca
    25501 gtttgtatgc tttcgggatt ggtttgatct tcccgacttt attccgcttt acgctttttt
    25561 ccaataactt accgaaaggg accgtctccg catcgctgaa tatggtgatc ctgatggtga
    25621 tgtctgtctc ggtcgaaatc ggccgctggc tgtggtttaa cggcggtcgc ttgccgtttc
    25681 atctgttagc cgtcgtggcg ggcgttatcg tcgttttcac cctggcggga ttgctcaatc
    25741 gcgtgcgcca acatcaggct gccgagctgg cggaggagcg gtgatttttg cgcgatcctg
    25801 ccgtcaggct ctattcttaa cgttatgaat aaactcattg aactcagacg cgccaaaatg
    25861 ttggcgctct ctttactgct tatcgccgct gctacctttg tcgttacgct gtttttgccg
    25921 cccaattttt gggtgagcgg cgtgaaggcg attgctgaag cggcgatggt cggcgcgctg
    25981 gcggactggt ttgcggtggt ggcgctgttt cgccgcgtgc cgattccgat catttctcgt
    26041 catacggcga ttatcccgcg taataaagac cggattggcg aaaatctcgg ccagttcgtg
    26101 caggaaaaat ttctcgatac ccagtcgctg gtggcattga ttcgacgtca cgaaccggcg
    26161 ttgttgattg gcaactggtt tagtcagccg gagaacgccc gccgcgttgg tcagcatctg
    26221 ttgcagatca tgagcggttt tcttgaactg accgatgatg cacgtattca gcgcctgctt
    26281 aagcgcgcgg tccatcgggc gattgataag gtcgatcttt ccggaaccag tgcgttgatg
    26341 ctggagagta tgaccaaaaa cgatcgtcat caggtgctgc tcgatacgct gatcgcacag
    26401 ttgatcgccc ttctccagcg cgataaatcg cgcaagttta ttgcccagca gattgttcgc
    26461 tggctggaga gtgagcatcc actgaaagcc aaaattctcc ccaccgaatg gttgggcgaa
    26521 catagcgcgg agttggtttc tgacgcggtg aattctttgc ttgatgatat cagccgtgat
    26581 cgtgcgcatc agatccgtca tgcgtttgat cgcgccactt ttgccctgat cgacaagttg
    26641 aaaaacgatc cggaaatggc agcgcgagcc gatgccgtaa aaagttatct gaaagaagat
    26701 gaagctttta accgctatct cagtgaattg tggggggatt tacgggaatg gctgaaagcg
    26761 gatatcaaca gtgaagattc tcgtgtgaaa gaacgtatcg cgcgggcggg tcaatggttt
    26821 ggcgaaacgt taattgccga tgatgccttg cgggcatcgt taaatggtca cctggaacaa
    26881 gccgcgcacc gcgtcgcgcc tgagttttcc gcattcctga cgcgccacat cagcgataca
    26941 gtaaaaagct gggatgcgcg ggatatgtcg cggcaaatcg agttaaatat cggcaaagat
    27001 ctgcagttta tccgtgtcaa cggtacgctg gttggcggtt gtattgggct aattttatat
    27061 ttgttgtcgc agctcccggc cttgttcccc ctcagcaatt tttagaaatc catgaaaaac
    27121 taagtggcta accgcgagag agatcaaata aaccacacta aaagaatggt aataatgtgg
    27181 cctcttttca ttatctcccg tggtacgggg aaggaaaatc atgtcactta tcaccgatct
    27241 acccgccatt ttcgatcagt tctctgaagc tcgccagaaa ggctttctca ccgtcatgga
    27301 tctcaaggag cgcggcattc cgctggttgg cacttactgc acctttatgc cgcaagagat
    27361 cccgatggca gccggtgcgg ttgtggtttc gctctgttct acctctgatg aaaccattga
    27421 agaagcggag aaagatctgc cgcgcaacct ctgcccgctg attaaaagca gctacggctt
    27481 cggcaaaacc gataaatgcc cctacttcta cttttcggat ctggtggtcg gtgaaaccac
    27541 ctgcgacggc aaaaagaaaa tgtatgaata catggcggag tttaagcccg ttcatgtgat
    27601 gcagttgccg aacagcgtta aggacgatgc ctcgcgtgcg ttatggaaag ccgagatgct
    27661 gcgcttgcaa aaaacggtag aagaacgttt tgggcacgag attagcgaag atgctctgcg
    27721 cgatgccatt gcgctgaaaa accgcgaacg tcgcgcactg gccaattttt atcatcttgg
    27781 gcagttaaat cctccgccgc ttagcggcag cgacattctg aaagtggtct acggcgcaac
    27841 cttccggttc gataaagagg cgttgatcaa tgaactggat gcaatgaccg cccgcgttcg
    27901 tcagcagtgg gaagaaggcc agcgactgga cccgcgtcca cgcattttaa tcaccggctg
    27961 cccgattggc ggcgcagcag aaaaagtggt gcgggcaatt gaagagaatg gcggctgggt
    28021 tgtcggttat gaaaactgca ctggggcgaa agcgaccgag caatgcgtgg cggaaacggg
    28081 cgatgtctac gacgcgctgg cggataaata tctggcgatt ggctgctcct gtgtttcgcc
    28141 gaacgatcag cgcctgcaaa tgctcagcca gatggtggaa gaatatcagg tcgatggcgt
    28201 agttgatgtg attttgcagg cgtgccatac ctacgcggtg gaatcgctgg cgattaaacg
    28261 tcatgtgcgt cagcagcaca acattcctta tatcgctatt gaaacagact attccacctc
    28321 agatgtcggg cagctcagta cccgtgtcgc ggcctttatt gagatgctgt aaggagtggc
    28381 agtgacatat tcgattggca ttgattctgg ttctaccgcc accaaaggga tcttactggc
    28441 agacggcgtg attacgcgcc gtttcctcgt tccaacaccc tttcgtccgg caacagcaat
    28501 tactgaagcc tgggaaactc tgcgcgaagg gttagagaca acgccgtttc tgacgctcac
    28561 cggctatgga cggcaactgg tggattttgc cgataaacaa gtgacggaaa tctcctgtca
    28621 tgggctgggc gcacggtttc ttgcgccagc aacgcgcgcg gtaatcgaca tcggtggtca
    28681 ggacagcaaa gtgattcagc ttgatgatga cggtaacctg tgcgatttcc tgatgaatga
    28741 caaatgcgcg gcgggcaccg ggcgtttcct ggaggtgatc tcgcgcacgc ttggcaccag
    28801 cgtcgagcaa ctcgacagca ttaccgaaaa tgtcacgccg cacgccatca cgagtatgtg
    28861 cacagtgttt gccgaatcag aagtgatcag cctgcgctca gcgggcgtcg cgccagaagc
    28921 gattctcgcc ggagtgatta acgcgatggc gcggaggagt gccaatttca ttgctcgtct
    28981 ctcctgtgaa gcgccgattc tgtttactgg tggcgttagt cattgccaga cgtttgcccg
    29041 gatgctggaa tctcatctgg gaatgccggt aaatacccat cctgatgcgc aatttgctgg
    29101 cgcaattggc gcggcggtaa ttggtcaacg agtgaggaca cgccgatgaa agagttttta
    29161 tttttatttc actccacagt cggcgtcata caaacccgca aagcgttgca ggcagcgggc
    29221 atgacttttc gcgtcagcga tattccccgt gatttacgcg gcggctgcgg gttatgcatt
    29281 tggctgacct gtccccccgg cgaggaaata caatgggtga tccctgggct gactgagtca
    29341 atttattgtc agcaggatgg tgtctggcgc tgcattgcac attaccgggt ttctccgcgt
    29401 taaggattca ttaaggtcgc tacgtatact tctcgatatc ttattgattt gccttaaagg
    29461 gattaccgtg acaaaaagta tttcgttaag taaacgcatc tccgttattg tgattctttt
    29521 tgccattgtg gcagtgtgta ctttcttcgt gcaatcttgc gcccgtaaaa gcaatcatgc
    29581 agcatcattt cagaattacc acgccaccat tgacggcaaa gaaattgcag gcataacgaa
    29641 taatatctct tcgctgacct ggtcggcaca aagcaatact ctttttagca ctatcaacaa
    29701 acctgccgcc attgttgaaa tgaccaccaa tggtgatttt atccgcacaa ttcctctgga
    29761 ttttgttaag gatctcgaga cgatcgaata cattggcgat aatcagtttg tcattagcga
    29821 tgagcgcgat tacgccattt atgtgatttc actgacccca aactcggaag taaaaatcct
    29881 caaaaaaata aaaatcccgc tgcaagactc cccaaccaat tgcggttttg aagggttggc
    29941 ttattctcgc caggatcaca ctttctggtt ttttaaagag aaaaatccga ttgaggtcta
    30001 caaagttaac gggctgttaa gtagcaatga attacacatc agcaaagacg aggcactaca
    30061 acggcaattt actttagatg atgtgtccgg cgcagaattt aaccagcaaa aaaatacgct
    30121 tctggtgctg tctcatgaat ctcgcgcact ccaggaagta accctggttg gcgaggttat
    30181 cggtgaaata tcactaacca aaggtagtcg aggtttgtcg cataacatca aacaggccga
    30241 agggattgcg atggatgcct ctggtaatat ttatatcgtt agcgaaccaa atcgttttta
    30301 tcgctttacc cctaaatcat cgcattaaca ggctggcata tttcaccttt ttgttattaa
    30361 cttgttaaca acaaaaatga catacaaggt taaacatgcc ttcgtcctcg catcccgtag
    30421 aacgcttttc tttcagcacc gcgcttttcg ggatgctggt tctgacctta ggtatgggat
    30481 taggtcgctt cctttatacg cctatgttgc ccgtcatgat ggcggaaggg gcgttttcat
    30541 ttagccagct ctcgtggatt gccagcggca actatgcggg gtatctggct ggcagtctgc
    30601 tattttcgtt tggcgcattt caccagccgt cgcgcctgcg cccatttctg ttggtttctg
    30661 ccctggcgag cgggttgttg atcctcgcaa tggcatggtt gccgccattt attctggtgt
    30721 tattgattcg cttcctggcg ggtgtcgcca gcgccggtat gctgattttt ggttcgacgc
    30781 tgattatgca gcacacgcgc catccttttg tgctggcagc tttgttttct ggcgttggca
    30841 ttggcattgc actgggcaac gaatatgttc tggcaggcct gcattttgcc ttatcctcgc
    30901 aaacgttatg gcaaggcgcc ggggcgcttt ctggcatgat gctgattgca cttacgcttt
    30961 taatgccctc gaaaaaacac gccatcgcac caatgccatt ggcaaaaacg gagcaacaga
    31021 taatgagctg gtggttactg gctattctgt acggcctggc gggatttggt tatatcatcg
    31081 tcgcgactta cctgccgctc atggcgaaag atgcaggctc accattgtta accgcccatc
    31141 tctggacgtt agtcggctta tcaattgtgc caggctgctt tggctggcta tgggcagcaa
    31201 aacgttgggg ggctttgcct tgcctgacgg cgaatttgct ggtgcaggct atcagtgtgc
    31261 tgcttactct cgccagcgac tctcctctct tgcttatcat tagtagtatc ggttttggcg
    31321 gtacctttat gggcacgaca tctctggtga tgactatcgc ccgccaactg agcgttccgg
    31381 gaaatctcaa tcttttaggc tttgtgacgc tcatttacgg cattggacaa atcctcggcc
    31441 cggcgctgac cagtatgctc ggcaatggaa catcggcgct tgccggcgcc actctctgcg
    31501 gcgcggcggc gttgtttatt gctgcgctta tcagcacagt acaattattt aagctgcaag
    31561 tggtcacttc atgaggagtg tttcccttac cactcaagaa attcgaccgc aaccgttgat
    31621 gtcagccaga ctccgttttc cgcttgccag aaagggatac ctcgtttcgc catttcctgt
    31681 gctttgacgg ttaagataac cggcgatccg tgtcttgctc ccactttgcg tgccgttgct
    31741 tcatcagcag agagatgcac gtaatgacga tcccctgcaa tcagcccctg tttttttatc
    31801 tcatcaagga aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt tttttcgaca
    31861 aaggaaatag cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca cctaccatca
    31921 ctggaataac taaaacgttt tttatcactg gtagcgacca cggtatccag caatgcacgg
    31981 gttaaccgct tgcccgcttt ttgggcacag agtataagct tatcgatatc cgcccatcct
    32041 tcacggtcaa gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa acttaaaaac
    32101 ttgctcgtat ccgccagctc tttttcgttg tactttgcca tctcgttttc gtccctggat
    32161 tttctgtttt tatttactga tgtcattgtc actgggttta tgaattttct gcaactcaat
    32221 cctgcgcaga gccttttcca cacagcgtaa tttttccgct atgcaaaaaa cgcatagcca
    32281 gaaacatact tgcattttcc ccatacccac atggctgata aggtttttcc gctccaggaa
    32341 ttttcctggc tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt atgacacaac
    32401 aaggggatgc tgtagccggg gagctggcga cagaaaaagt gggcattaaa gggtatctgg
    32461 ctttttttct caccataatc tttttttccg gtgttttttc cgggacagac agttggtggc
    32521 gcgtttttga tttttccgtt ctgaacggat cgtttgggca actgcctggt gcgaatggag
    32581 ccaccacctc atttcgcggg gcgggtggcg cgggggctaa agatggcttt ctctttgcgc
    32641 tggagctggc accgtcagtc attctctctc tggggattat ttcaattacc gatggtcttg
    32701 gtggattacg cgccgcgcag cagttaatga cacctgtgct aaaaccgttg ttgggcattc
    32761 cagggatctg ctcgctggcg ctgatcgcta acctgcaaaa cacggatgcc gccgccggta
    32821 tgaccaaaga gctggcgcag gaaggtgaaa ttaccgaacg cgataaagtg attttcgccg
    32881 catatcaaac cagcggtagc gccatcatca ccaactactt ctcttccggc gtcgcggtgt
    32941 ttgcctttct gggcacgtcc gtaatcgtac ctttagccgt catcctggtg tttaaatttg
    33001 tcggagccaa cattttgcgc gtctggctta acttcgaaga acgtcgcaac ccaacgcaag
    33061 gagcgcaagc atgacaactc aggtccgcaa aaatgtcatg gatatgttta tcgatggtgc
    33121 gcgccgtggc tttactatcg ccacgaccaa cctgctgcca aacgtggtga tggcgtttgt
    33181 catcattcag gcgctgaaaa ttaccggcct gctcgactgg gtggggcata tctgtgaacc
    33241 cgtaatggcg ctgtggggat tacccggtga agcggcaacc gtccttttag ccgcattaat
    33301 gagtatgggc ggcgcagtcg gtgttgccgc cagtctggcg accgccggcg cattaaccgg
    33361 tcacgatgtc accgttttac tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt
    33421 cggtcgctgc ctggggacag ccgaagtgaa tgccaaatat tacccgcata tcattacggt
    33481 ctgcgtgatt aacgcattgc tgtcgatctg ggttatgcag cttattgttt aacaaggagt
    33541 taccatgatt gattataccg cagccggttt taccctgctg cagggagcgc atttgtatgc
    33601 gccggaagat cggggaattt gcgatgtcct cgtcgctaac ggcaaaatta tcgccgttgc
    33661 cagcaatatc ccttctgaca ttgtaccgaa ctgcacggtt gtcgatctca gtgggcagat
    33721 cctctgccca ggttttattg atcaacacgt ccatttgatt ggcggtggcg gcgaagcagg
    33781 tcccacgacg cgcacgccgg aagtggcgct aagtcgcctg acggaagcgg gcgtcacgtc
    33841 agtggttggt ctgctgggca ccgactctat ctctcgccac ccggaatccc tgctcgccaa
    33901 gacccgtgcg ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg cttatcatgt
    33961 cccttcccgc accattacgg gttccgtgga aaaagacgtg gcgattatcg atcgtgtgat
    34021 tggcgtgaaa tgcgccatct ctgatcaccg ttctgccgca ccggacgttt atcacctggc
    34081 caatatggcg gcagaatccc gcgttggcgg tttgctcggc ggtaaacctg gcgtcaccgt
    34141 gttccacatg ggcgacagta aaaaggcgtt acagcctatt tatgacctgc tggaaaactg
    34201 cgatgtgccg atcagcaagc tgctgccgac ccacgttaac cgcaacgtac cgttgtttga
    34261 gcaggcgctg gagttcgcgc gcaaaggcgg caccatcgat atcaccagca gcattgacga
    34321 accggtcgcc cctgccgaag gtattgcccg cgccgttcag gcgggtattc cgctggcacg
    34381 cgtcaccctc agctccgacg gcaacggtag ccagccgttc ttcgatgacg aagggaattt
    34441 aacccatatc ggtgttgcag gttttgaaac gttgctggaa accgtgcagg tgctggtcaa
    34501 agactatgat ttcagtatca gcgatgccct gcgcccgctc accagtagcg tagccggttt
    34561 ccttaacctg accgggaaag gcgaaattct gccaggcaat gatgctgact tgctggtcat
    34621 gacgccagaa ctgcgcattg agcaggtata cgctcgcggc aaactgatgg tcaaagacgg
    34681 caaagcctgc gtgaaaggaa cgtttgaaac ggcttaaacg gttgtcagcc gcccgaatgg
    34741 acgtagaatg cccgcaaggg cggctgacag agtaaaacgt aatggatgac tgtggtgcga
    34801 ttttgcataa tattgaaacc aaatggcttt atgattttct gaccctggaa aaatgccgca
    34861 atttttccca ggcggcagtc agtcgcaacg tctcgcaacc ggcattcagc cgccgcatcc
    34921 gtgcgctgga acaggcgatt ggtgttgaat tgtttaaccg ccaggtaacg ccgctgcaac
    34981 tctcagaaca agggaaaatc ttccattcgc agatccgcca tctgttgcaa cagttagaga
    35041 gcaatctggc agagctgcgt ggcggcagcg attacgcgca acgtaaaatc aagatagccg
    35101 ctgcacactc tctttccctc ggtctgttac cgtccattat cagccagatg ccgccgctct
    35161 ttacctgggc aattgaagcc attgatgtcg atgaagcggt cgataaactg cgtgaagggc
    35221 aaagtgactg tattttttcc tttcacgacg aagatttgct ggaagcgccg tttgaccaca
    35281 ttcgcttatt tgaatctcaa ttgttccctg tctgcgccag tgacgaacac ggagaagcac
    35341 tttttaacct cgcgcagcca cactttccgt tactgaatta cagccgcaac tcctacatgg
    35401 ggcgattgat taatcgcacc ctgacgcgcc acagtgagtt aagtttcagc accttttttg
    35461 tctcttcgat gagcgagctt ttaaagcagg ttgccctcga cggctgtggg attgcctggc
    35521 tgccggagta cgccatccaa caagaaattc gcagcgggaa gctcgttgta ctgaatcggg
    35581 acgaactggt gatcccgatt caggcctatg cataccggat gaatacccgc atgaatcccg
    35641 ttgccgaacg cttctggcgt gaactgcgcg agctggagat tgtgcttagc tgacattctc
    35701 cagcgtcgca ctgcgccaac cgccattatc ccagaccaga tcaaacgtta gcggttcatc
    35761 gtcgtggtac agcattatct caaggctgat cgccagagtg tggcagtacg cttctttcag
    35821 attaacctgc aacgccgcaa tccgtggctc atgaaataac agtgcagaac gcagctgacg
    35881 gcaataccag ctctggagat gtttgtcgct cgggttatgc acttgccact cgggcatgcc
    35941 tgaaacaagt aatgacggta atgccgaatg aggagaatta aaaagagtga ggagatcgct
    36001 gcataccgat ttacgcagca atgacgtttt attgcctgaa attttaggta aaacagcctg
    36061 aagtgattgg cgcatcattt tgcgcactcc tgacgtttag caacatatgc ggcttgccct
    36121 gaacaccggg caccactgta ttgatgctca ttttccatag ccattccctt ttgttcatta
    36181 catccatgtc agtctgagac aggcctgaat cctgtcagca aagtataaaa ccttacttgc
    36241 ctatgaatat ctactgactt ttcaggaaaa aagtcatgat ttgataaaaa tcatcaaact
    36301 caaaaaaaaa ccgctagtat attctgaata ttccgaatag ttacgtagat gaaaatactt
    36361 gtcaccgctg ctacttaatt ccggtggga
//
LOCUS       DAFNBJ010000029        36162 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000029 DAFNBJ010000000
VERSION     DAFNBJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.029"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(65..205)
                     /locus_tag="L2X49_001425"
     CDS             complement(65..205)
                     /locus_tag="L2X49_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796536.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            301..1017
                     /gene="arcA"
                     /locus_tag="L2X49_001426"
     CDS             301..1017
                     /gene="arcA"
                     /locus_tag="L2X49_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBN4796537.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1076..2428)
                     /gene="creD"
                     /locus_tag="L2X49_001427"
     CDS             complement(1076..2428)
                     /gene="creD"
                     /locus_tag="L2X49_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBN4796538.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2486..3909)
                     /gene="creC"
                     /locus_tag="L2X49_001428"
                     /pseudo
     CDS             complement(2486..3909)
                     /gene="creC"
                     /locus_tag="L2X49_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
     gene            complement(3909..4598)
                     /gene="creB"
                     /locus_tag="L2X49_001429"
     CDS             complement(3909..4598)
                     /gene="creB"
                     /locus_tag="L2X49_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBN4796539.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4611..5084)
                     /gene="creA"
                     /locus_tag="L2X49_001430"
     CDS             complement(4611..5084)
                     /gene="creA"
                     /locus_tag="L2X49_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBN4796540.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5295..6164
                     /gene="robA"
                     /locus_tag="L2X49_001431"
     CDS             5295..6164
                     /gene="robA"
                     /locus_tag="L2X49_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBN4796541.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTRHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6161..6808)
                     /gene="gpmB"
                     /locus_tag="L2X49_001432"
     CDS             complement(6161..6808)
                     /gene="gpmB"
                     /locus_tag="L2X49_001432"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBN4796542.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6860..7372
                     /gene="yjjX"
                     /locus_tag="L2X49_001433"
     CDS             6860..7372
                     /gene="yjjX"
                     /locus_tag="L2X49_001433"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HBN4796543.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(7415..7741)
                     /gene="trpR"
                     /locus_tag="L2X49_001434"
     CDS             complement(7415..7741)
                     /gene="trpR"
                     /locus_tag="L2X49_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBN4796544.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7831..9768)
                     /gene="sltY"
                     /locus_tag="L2X49_001435"
     CDS             complement(7831..9768)
                     /gene="sltY"
                     /locus_tag="L2X49_001435"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBN4796545.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            9979..11646
                     /gene="ettA"
                     /locus_tag="L2X49_001436"
     CDS             9979..11646
                     /gene="ettA"
                     /locus_tag="L2X49_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBN4796546.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11953..13185)
                     /gene="nadR"
                     /locus_tag="L2X49_001437"
     CDS             complement(11953..13185)
                     /gene="nadR"
                     /locus_tag="L2X49_001437"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBN4796547.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13206..14588)
                     /gene="radA"
                     /locus_tag="L2X49_001438"
     CDS             complement(13206..14588)
                     /gene="radA"
                     /locus_tag="L2X49_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBN4796548.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14637..15605)
                     /gene="serB"
                     /locus_tag="L2X49_001439"
     CDS             complement(14637..15605)
                     /gene="serB"
                     /locus_tag="L2X49_001439"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBN4796549.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            15711..16355
                     /locus_tag="L2X49_001440"
     CDS             15711..16355
                     /locus_tag="L2X49_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBN4796550.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16383..17399
                     /gene="lplA"
                     /locus_tag="L2X49_001441"
     CDS             16383..17399
                     /gene="lplA"
                     /locus_tag="L2X49_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBN4796551.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17431..17580
                     /locus_tag="L2X49_001442"
     CDS             17431..17580
                     /locus_tag="L2X49_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796552.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(17855..18574)
                     /gene="deoD"
                     /locus_tag="L2X49_001443"
     CDS             complement(17855..18574)
                     /gene="deoD"
                     /locus_tag="L2X49_001443"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBN4796553.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18654..19877)
                     /gene="deoB"
                     /locus_tag="L2X49_001444"
     CDS             complement(18654..19877)
                     /gene="deoB"
                     /locus_tag="L2X49_001444"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBN4796554.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(19929..21251)
                     /gene="deoA"
                     /locus_tag="L2X49_001445"
     CDS             complement(19929..21251)
                     /gene="deoA"
                     /locus_tag="L2X49_001445"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBN4796555.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21378..22157)
                     /gene="deoC"
                     /locus_tag="L2X49_001446"
     CDS             complement(21378..22157)
                     /gene="deoC"
                     /locus_tag="L2X49_001446"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBN4796556.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22415..23965
                     /locus_tag="L2X49_001447"
     CDS             22415..23965
                     /locus_tag="L2X49_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBN4796557.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            23937..24800
                     /locus_tag="L2X49_001448"
     CDS             23937..24800
                     /locus_tag="L2X49_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBN4796558.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(25115..25897)
                     /locus_tag="L2X49_001449"
     CDS             complement(25115..25897)
                     /locus_tag="L2X49_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBN4796559.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(25894..26967)
                     /locus_tag="L2X49_001450"
     CDS             complement(25894..26967)
                     /locus_tag="L2X49_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBN4796560.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27089..27250)
                     /locus_tag="L2X49_001451"
     CDS             complement(27089..27250)
                     /locus_tag="L2X49_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBN4796561.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27377..27982)
                     /gene="osmY"
                     /locus_tag="L2X49_001452"
     CDS             complement(27377..27982)
                     /gene="osmY"
                     /locus_tag="L2X49_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBN4796562.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28375..29964)
                     /gene="prfC"
                     /locus_tag="L2X49_001453"
     CDS             complement(28375..29964)
                     /gene="prfC"
                     /locus_tag="L2X49_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBN4796563.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVQASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30055..30732)
                     /gene="yjjG"
                     /locus_tag="L2X49_001454"
     CDS             complement(30055..30732)
                     /gene="yjjG"
                     /locus_tag="L2X49_001454"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBN4796564.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30747..31193)
                     /gene="rimI"
                     /locus_tag="L2X49_001455"
     CDS             complement(30747..31193)
                     /gene="rimI"
                     /locus_tag="L2X49_001455"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBN4796565.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31162..31575)
                     /gene="holD"
                     /locus_tag="L2X49_001456"
     CDS             complement(31162..31575)
                     /gene="holD"
                     /locus_tag="L2X49_001456"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBN4796566.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31678..32709
                     /gene="rsmC"
                     /locus_tag="L2X49_001457"
     CDS             31678..32709
                     /gene="rsmC"
                     /locus_tag="L2X49_001457"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBN4796567.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            32977..33063
                     /locus_tag="L2X49_001458"
     tRNA            32977..33063
                     /locus_tag="L2X49_001458"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33011..33013,aa:Leu,seq:cag)
     gene            33092..33178
                     /locus_tag="L2X49_001459"
     tRNA            33092..33178
                     /locus_tag="L2X49_001459"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33126..33128,aa:Leu,seq:cag)
     gene            33213..33299
                     /locus_tag="L2X49_001460"
     tRNA            33213..33299
                     /locus_tag="L2X49_001460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33247..33249,aa:Leu,seq:cag)
     gene            complement(33338..33574)
                     /locus_tag="L2X49_001461"
     CDS             complement(33338..33574)
                     /locus_tag="L2X49_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBN4796568.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            33715..34503
                     /gene="fhuF"
                     /locus_tag="L2X49_001462"
     CDS             33715..34503
                     /gene="fhuF"
                     /locus_tag="L2X49_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBN4796569.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(34541..35218)
                     /gene="bglJ"
                     /locus_tag="L2X49_001463"
     CDS             complement(34541..35218)
                     /gene="bglJ"
                     /locus_tag="L2X49_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBN4796570.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(35176..35901)
                     /locus_tag="L2X49_001464"
     CDS             complement(35176..35901)
                     /locus_tag="L2X49_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN4796571.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT         8611 a         9869 c         9131 g         8551 t
ORIGIN      
        1 caattgaact tgatatatgt caacgaagcg tagttttatt gggtgtccgg cccctcttag
       61 cctgttatgt tgctgttaaa atggttagga tgacagccgt ttttgacact gtcgggtcct
      121 gagggaaagt acccacgacc aagctaatga tgttgttgac gttgatggaa agtgcatcaa
      181 gaacgcaatt acgtacttta gtcatgttac gccgatcatg ttaatttgca gcatgcatca
      241 ggcaggtcag ggacttttgt acttcctgtt tcgatttagt tggcaattta ggtagcaaac
      301 atgcagaccc cgcacattct tatcgttgaa gacgagttgg taacacgcaa cacgttgaaa
      361 agtattttcg aagcggaagg ctatgatgtt ttcgaagcga cagatggcgc ggaaatgcat
      421 cagatcctct ctgaatatga catcaacctg gtgatcatgg atatcaatct gccgggtaag
      481 aacggtcttc tgttagcgcg tgaactgcgc gagcaggcga atgttgcgtt gatgttcctg
      541 actggccgtg acaacgaagt cgataaaatt ctcggcctcg aaatcggtgc agatgactac
      601 atcaccaaac cgttcaaccc gcgtgaactg actattcgtg cacgcaacct gctgtcccgt
      661 accatgaatc tgggtactgt cagcgaagaa cgtcgtagcg ttgaaagcta caagttcaat
      721 ggttgggaac tggacatcaa cagccgttcg ttgatcggcc ctgatggcga gcagtacaag
      781 ctgccgcgca gcgagttccg cgccatgctt cacttctgtg aaaacccagg caaaattcag
      841 tcccgtgctg aactgctgaa gaaaatgacc ggccgtgagc tgaaaccgca cgaccgtact
      901 gtagacgtga cgatccgccg tattcgtaaa catttcgaat ctacgccgga tacgccggaa
      961 atcatcgcca ccattcacgg tgaaggttat cgcttctgcg gtgatctgga agattaatcg
     1021 gctttaccac cgtcaaaaaa acggcgcttt ttagcgccgt ttttattttt caaccttatt
     1081 tccagatacg taattcatcg tccgttgtaa cttctttact ggctttcatt ttcggcagtg
     1141 aaaacgcata ccagtcgata ttacgggtca caaacatcat gccggccagc gccaccacca
     1201 gcacactggt tcccaacaac agcgcgctat cggcagagtt gagcagtccc cacatcacac
     1261 catccagcaa caacagcgcg agggtaaaca acatgctgtt gcgccagcct ttcaataccg
     1321 cctgcaaata aataccgttc attaacgccc caatcagact ggcgattatc catgccacgg
     1381 taaaaccggt atgttcagaa agcgccagca agagcaaata aaacatcacc aatgaaagcc
     1441 ccaccagcaa atattgcatt gggtgtaagc gttgcgcggt gagcgtttca aaaacaaaga
     1501 acgccataaa agtcagcgca atcagcagaa tggcatactt agtcgcccgg tcagttaatt
     1561 ggtattgatc ggctggcgtc gtcactgcga cgctaaacgc ggggaagttt tcccagccgg
     1621 tatcattgcc tgaagcaaaa cgctcaccga gattatttgc aaaccagctg ctttgccatt
     1681 gcgcctgaaa acctgactcg ctaacttccc gtttggctgg caaaaaatca cctaaaaaac
     1741 tgggatgcgg ccagttgctg gttaaggtca tttcgctatt acgcccggca ggcaccacag
     1801 aaagatcgcc ggtaccgctt aaattcaggg ccatattcag cttcaggttc tgcttgcgcc
     1861 agtccccttc gggtaaaggg atatgcacgc cctgcccgcc ttgctctaac ccggtaccgg
     1921 gttcgatggt cagcgctgtt ccgttaactt caggcgcttt caccacacca ataccacgcg
     1981 catccccgac gctaatcaca ataaatggct tgcctaaggt gatatttggt gcgtcgagtt
     2041 cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa atcactgtgc cagacctgac
     2101 cggtataaat ccctatcttg cgttcttcaa cgttctgatt gccatcaacc atcaatgact
     2161 caggtaacca aaaatggata aaacttcgtt tccgctccac ggttttatcg tcttcctgca
     2221 ccgtataaag ctcggtcaca ggaatagcga tgagcggccc aacgagtttt tgcggcccgc
     2281 tggtactttg gcgaatcgca tcttccacat cgctacggta atcggcacgt tcgacaatca
     2341 cctgccgaat cagcattatc ggaatcaaca acagcaatac tgcaccaaac aaggtggtca
     2401 ttttccagaa caggggggat ttcaacatag tcttctcctt tgcaatggca gcaggatacg
     2461 aaggctatgt gggaagaatt tgaagctatg tgaagtgacg gtgaagtcgt agcgaggcca
     2521 gcacgccacc ttcctgcacg ttgcgcagcg tgacttcgcc gttaaacaaa cgggcgacct
     2581 cactgacgaa cgccaacccc agaccgctgc ttttttgccc atttgcacgc ggcaaagagt
     2641 aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat accactaccg gtatccagca
     2701 ccttaagggc gacgtgttcc tgatccactt cggcgcttag cgttatgcga ccgctctcgg
     2761 gggtaaaatc gatggcgtta tccagtaaat tccccagcgc ctgctccagt aacgccggtt
     2821 cagcagcaac attaacctca gtgggcataa catgtagagt gatgtttttt tctgccaact
     2881 gcacggtgcg cgcttcgctg acgcggcgaa ataatgccgc cacatcaaca acagtcagaa
     2941 cgacttcctg acgattctcc agtcttgcct ggcgtagtaa cgtttctacc aatgcctgca
     3001 tacgcgcatt ttgcgtcaga atgttgtcgg taaaacgagc accacttccg gtggcggacc
     3061 ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta gctcatgggt
     3121 taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc gcatactttc
     3181 cagcgcctgc gcaagcttac gcaactcgct actgccgagt tcaggaagag gaacgggctt
     3241 attgtcagtg acggaatcgg catagcgagt gagccgggca atggagcggt tgatccacca
     3301 aaccatgcct gcgccaatca ccagtgcaat ccccagcaaa atggcgctgg cccataaaat
     3361 tcgccgctcg ctacgcttaa tgaccggagc catcgccgcg ttcggtttgc ctacgctcaa
     3421 aacgccaata agccgcgagc cgtccataat cggcgcggca acatacatca ccgaactttc
     3481 gggatcggca ggattttgca acgtgctgcg cgcaccatac tgaccacgca acgttagcca
     3541 gacgtcattc cagcgcgaat aatcctgccc aacggcttta tttgccgaat cgaacaatac
     3601 tttaccctgc gaatcggtca tatagacatg atattcattg cgcactttgt taatgccacc
     3661 gatattggcg cgaaacgggc gatgttgtag ctgattaaac gcctgcgcca gttgcccatg
     3721 cgttgggtcc ccagagagca aatcgggacg cgccagctcc gccagcaacg ttgcggtatc
     3781 gattaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga caaaaatagc
     3841 cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca accgcatgcc
     3901 gatacgcatt acaggcccct caggctatat cccatgccgc gatgagtatt aatcggtgaa
     3961 agatcggggt tgatggcgcg cagcttggca cgcaacgttt taatgtgggt atcgacggtg
     4021 cgatcgtagg tgtcctgcgc atcttcccac acgctatcca ttagttgctg gcgggaccag
     4081 acgcggcctg gcgacttgag taacgtcttc agcaataaaa attcataccg cgtcagcgtt
     4141 aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa atgaccaata
     4201 cggatgacgg gagacggcga cgagaacttc ttcacccgac gcagtaaggt gcgcaccctg
     4261 gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcagcgcc aatttccagc
     4321 ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg taacgccgga
     4381 tggagcgcca gtaactggcg gcacaattca aagccgctga tatcaggcaa accaacatcg
     4441 agaatcatga cgtcgggaac ctgctggcga gctttatcca gcaccggcaa gcctcgctca
     4501 aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc ggctatccct
     4561 tgctcatctt ccactaacca gaccgtttcc cgttgcataa atcgcctctg ttattgccgc
     4621 cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac aactttgtcg
     4681 gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac gacctgtaac
     4741 gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc tttgccgttt
     4801 ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatagc cgcatcggag
     4861 gtatcttccg ccagacccaa tcccccttta ataccacctg ttttcgcccg gctcacataa
     4921 caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt gtgatccggg
     4981 ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc caatggcccc
     5041 agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt accattttta
     5101 tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag aacaaaatct
     5161 caatactttt atttccgtca agccctaaaa catactctac taagaaaaaa acactgaatg
     5221 ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg ttgttgaaag
     5281 gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc tggctggaag
     5341 gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt tattccaagt
     5401 ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg tatattcgtg
     5461 ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg attctggaca
     5521 tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc aagaagcagt
     5581 ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt ggtattcgcc
     5641 cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc ctggaagata
     5701 cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc tctgatttcc
     5761 gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg accattccgc
     5821 cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac gaacaagagg
     5881 tattctatac caccgcgtta gcccaggatc aggcagatgg ctatgtactg acgaggcatc
     5941 cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg ggaaccggcg
     6001 tgcaggagtt tatcctgaca gtatacggaa cgtgcatgcc aatgctcaac ctgacgcgcc
     6061 gtaaaggtca ggatattgag cgatactacc cggcagaaga cgccaaagcg ggagatcgcc
     6121 caattaatct gcgctgtgaa ctgctgattc ctatccgccg ttaacgctgc agctcatcta
     6181 atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacgacccag ccagacgcca
     6241 gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga cgtaagcggc
     6301 gctctgccca tgctggtaat ccgagaatcg tactcaccag gcatcccagt gcaataccat
     6361 gactcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc agtgccgcat
     6421 tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg tcaacggtgc
     6481 cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg atatgtcttt
     6541 tttccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg tcacagccgc
     6601 aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta ctgataatat
     6661 gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc tcacctttgg
     6721 cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac tgcgtttcac
     6781 cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg taacgttaat
     6841 tttctgagta atgctgatta tgcaccaagt tgtctgtgcg accaccaatc ccgctaaaat
     6901 tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc atattgcatc
     6961 cgttgccgtc gagagcggtg taccggaaca gccctttggc agtgaggaaa cgcgcgctgg
     7021 cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt tttgggtggc
     7081 gattgaagct ggcatcgatg gtgacagcac tttcagttgg gtggtgattg aaaacaccag
     7141 ccagcgcggc gaggcgcgtt cggctacttt gccgttacct gcggtgattc tgcagaaagt
     7201 gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat accggtattg atgagattgg
     7261 tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg ccagcgtcta
     7321 tcaccaggcg gtgatcctgg cgctgagtcc gtttcataat gccgtgtatt aagcgcctga
     7381 tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc gcttttcagc aacacctctt
     7441 ccagccactg gcgcagctcc acgggcgcgg ctttcaggct gttagatcca cgcgtaatcg
     7501 tcgcgatgcc cgcgccgagt tcatttttta actcacgctg actcatttcg ccacgcaaca
     7561 gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc gtcagcatca
     7621 ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg tcgacaaaac
     7681 gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat tgttgggcca
     7741 taatatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt actcgctaaa
     7801 gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt ccgtggcgct
     7861 catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcgtcataag ccagcacgtt
     7921 cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca ctgcgtcgat
     7981 acgcccggcg ctgttgccaa gccaggttcg cacccgccct ggtccggcgt tataagctgc
     8041 tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcagat aactggtgcc
     8101 aatgttgata ttcgtttccg gatccagcaa ttgcccaggg ctactataac cgggaataga
     8161 gaacatcttc accgtatggg tcgctgtacc aggcataatc tgcatcaggc cgctggcccc
     8221 taccggtgat ttcacttttg gattccaggc gctctcctga cgggcaatcg ccatcgcata
     8281 gctttgcggg atctccttac cgctggtgta gcgtttgaaa agatcgttgt aagccagcgg
     8341 gaatcgctct tccagatgat cccatagctt cccggcgatc gttgcctgaa cgctaagatc
     8401 ccaccattgg ttgttgaaag cataccgcgc cagttgagcc tgctctgttt ttgacttgct
     8461 cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt acatcaactc
     8521 gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct gcggcgcttt
     8581 atcaatcttc agctcatact cttctccgat gcgttgtgct gcaaccatcg ggtagaaacc
     8641 acgctgttgc atgagttgat gcaaaatctc tttcgcttca gcttcacgtc cgcgttccag
     8701 caataaatcc gcctgccagt aacgccattc atctttctct tttgcttcca tcggcagacg
     8761 cgccagccag gtattcaggc cgcgacgatc gccggtgcca agtgccattc gcacacggcg
     8821 ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca tcgcgccatt tcgcctgctc
     8881 gtcggtgaca tcgttgccca tcaaacgcca ggcgacgata tcgcgcagct cctgaatctg
     8941 atcttcatta agctgctgcg cctgggcaag cgacgggatc atcagccgtg cattttcagc
     9001 atcctgccgc gccacactgg caaacgccac cgctgccatt tgacgggtaa aatcggtcgc
     9061 tccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg aaatgattgc
     9121 cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta ccaggccagt
     9181 gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat cttgtttacc
     9241 tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct tgccggttag
     9301 ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc atttcgcata
     9361 gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg ctaacaagcc
     9421 acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg tgcgagcggg
     9481 aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt gattcatcag
     9541 atcatcggtg atctggcggt attccaggta gggataaagc ggataatcct tcagtccagg
     9601 catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttga tttgcgcgta
     9661 acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg tcagcagaca
     9721 gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa gcgcatcctc
     9781 taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct aaccagtcat
     9841 tgccgtttac gccacgttac ggacactttt ttacttttac tgcgaaggtg atcgaccaac
     9901 tgggattagg cggggaaaaa ggctacactt cgcctttgaa aactcatcat cacgataaac
     9961 atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt cggcaaagtt
    10021 gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc tggggcaaaa
    10081 attggtgtcc tgggtctgaa cggcgcgggt aagtctaccc tgctgcgcat tatggcgggc
    10141 attgataaag acatcgaagg tgaagcacgt ccgcagccag acatcaagat tggttatctg
    10201 ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg agtccattga agaagcggtt
    10261 tctgaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta cgccgatccg
    10321 gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat cattcaggct
    10381 cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg cggatgcgct acgtctgccg
    10441 gactgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt agcgttgtgc
    10501 cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa ccacctggat
    10561 gccgaatccg tggcctggct ggaacgcttc ctgcacgact tcgagggcac cgtggtggcg
    10621 attacccacg accgttactt cctcgataac gttgcaggct ggatcctcga acttgaccgc
    10681 ggtgaaggta ttccgtggga aggcaactac tcctcctggc tggagcagaa agatcagcgc
    10741 ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa agagctggag
    10801 tgggtacgtc agggaactaa aggccgccag tcgaaaggta aagcacgtct ggcacgcttt
    10861 gaagagctga acagcaccga atatcagaaa cgtaacgaaa ccaacgaact gtttattcca
    10921 cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa atcctacggt
    10981 gatcgcctgc tgattgatga cctgagcttc tcgatcccga aaggggcaat cgtcgggatc
    11041 atcggtccga acggcgcggg taaatcgacc ctgttccgta tgatctctgg tcaggaacag
    11101 ccggacagcg gcaccatcac tttaggtgaa acggtgaaac tggcatcggt tgatcagttc
    11161 cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg gctggatatt
    11221 atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt taactttaaa
    11281 ggggttgatc agggtaaacg cgttggtgaa ctttccggtg gtgagcgcgg tcgtctgcat
    11341 ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc aaccaacgac
    11401 ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc gggctgcgcg
    11461 atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacgcacat tctggattac
    11521 caggatgaag gtaaagttga gttcttcgaa ggtaacttta ccgagtacga agagtacaag
    11581 aaacgcacgc tgggcgcaga cgcactggag ccgaagcgta tcaagtacaa gcgtattgcg
    11641 aagtaatgcg taaaatgccg gatgcggcgt gaacgcctta tccggcctac aaaccaagaa
    11701 atttcaatac gttgtgaatc tccaggcctg ataagacgcg gtaagcgtcg catcaggcat
    11761 tgagcaccga atgccggata cggcgtgaac gccttttccg gcctacaaca gccttagtat
    11821 ccgcaaattc gtagcgggtc acccgtgctg attcttttaa accttgttta atctcgacag
    11881 cctcttccag agaggttttc aactcatcaa aaaacttatt ttatacctcg cctttgagcc
    11941 gtttcatcgc ggttatctct gctcccccat catctcccgc accagctcca cgcagcgcag
    12001 gaaacggctg tcataatcgt cctcttcaac ccgcacgaat tcgatattgt tctcttccag
    12061 catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga ggctgcgtaa
    12121 accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga aacggtattc
    12181 atcaatcaac gcctgtacga acggatgctc acgcccttcg tactttttgc agaacgcctg
    12241 ggtagtgaca aaatcagtgt caataaacgc cactttattg gcatatttca ctgcaaaatc
    12301 aatgtattgt gcgtggccca gcgcgatttt atcgtaatcg gaatactgca atgcgatctc
    12361 atcaccgccg aggtgtgaaa agacataatc gcgaccatat tcccacgcac tggtggtatt
    12421 gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc caccaaggat
    12481 cgccacggta cgtacaaaga acggcttcac ttccgtagga atatattccc agtagcggaa
    12541 cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg gatcgaccag
    12601 caccgtatcg atccccagat gttccatata ctgtggcgca tcggcttctt ccgaggtgta
    12661 gatcaggtcc ggctgaatcc ccttttcagc cataaacttt ttgatgccgt tgctccacac
    12721 atcccagccg tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa tgcgaatatt
    12781 tttctgatat ttaaaagttt gtaataacca gcgcagacga tccggcaccg tgggctgctg
    12841 cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac ccataatgat
    12901 atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc cggtatgcag
    12961 cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgtcggggaa attcaagccc
    13021 caaaaaacgg tgcaacgcct ccagcttttg cgcgctgggg cttttaattt tggcattcag
    13081 taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct gtagcgtgca
    13141 gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc cctccgtatc
    13201 tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac gccaaaaatc
    13261 tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa
    13321 ccgtgtttcg ccgcttctga aattcgttcc tgaccgctgg gcaccgggcg gatctcccct
    13381 gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc acgcaggctg
    13441 gaaaccatcg ccagcagtaa cgctaaatcg gcactggttt cggttacctt cacgccgccg
    13501 accacgttca caaatacatc ctgatcggcc atttgcagac caccgtgacg gtgcaagaca
    13561 gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg cggattcgcc
    13621 atcatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt tccttcccac
    13681 accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa aattgccgaa
    13741 gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag ctcattcacc
    13801 gcgccgaagc ggtttttatg gctgcgcaag gtgcgaaaac gggagtcggc atcaccatcc
    13861 agaagcaccg aacagtcgat acagtgttcc agcactttag ggccagccag cgagccatct
    13921 ttggttacgt gcccaaccat gacaatcgcc acaccgcgcg ttttggcgaa gcgcgtcaga
    13981 taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac atccgccata
    14041 tgcatcacct ggatcgagtc aattaccatc agtttcggtt gctcttcttc ggcaatcagg
    14101 cagatctgtt cgatgctggt ttccgacaac atattgagat tgtcagtcgg caggccaagg
    14161 cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cagtgacata cagcgttttc
    14221 atctgctggg ccagtttgca cagcgtttgc agtagcagcg tggatttccc cgcacctggg
    14281 ttaccgccaa tcagaatggc gcttcctggc accacgccgc cgcctagtac gcggtcgaac
    14341 tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga gagtttctgg
    14401 acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg cgccaccgtt
    14461 ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc actgcactgc
    14521 ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg ttttggagct
    14581 tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatgacgggc gagcaattac
    14641 ttctgattca ggctgcctga gaggatgcag aataccccca tcaggtcagc gtgacggatg
    14701 gtgacttccg tcttttcatt cacttttggc ttggcatggt aggcaatccc cagccctgct
    14761 gctttgatca tcggcaggtc attggctcca tcgccaatcg ccacggtctg cgccagcgga
    14821 atttcatact cttgcgcgag gcgggtcaga gttttcgctt tgtactgcgc gtcaacgatg
    14881 tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt ggctaccacg
    14941 gcggtcaggc gcagcttgtc gcgcaggtat tcagcaaaga aagtaaagcc gccggaggca
    15001 atcgccactt tccagcccag cgtttccagc ttgagcacca gttgcgttaa gcctggcatc
    15061 agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc
    15121 acacggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg ttcggttact
    15181 tccgccacca tctcgcccgt tccggccagt ttggcaattt catcaataca ttcaatctgg
    15241 atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg cgggattttc
    15301 cccagcgggg cgacatccag ttgcgcttca tgggccaggc gcgtagcccg tgcggtgagt
    15361 gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac aatcaccatc
    15421 gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc acgaccatac
    15481 agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc accacttaat
    15541 gaaagaggca gacccggcca taaagagaca tcttcaggca ggtcgcacca ggtaatgtta
    15601 ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga ggctaccttg
    15661 tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag atggctcgca
    15721 caaaactgaa attccggctg catcgggcag tgattgtcct gttctgtctt gccttgttag
    15781 tggcactgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag cgtaatccac
    15841 agctggaaga actggcccgc accctggcgc gtcaggtgac gctgaacgtt gcaccgctga
    15901 tgcgtaccga ctcaccagat gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg
    15961 aaagccgcat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc gcccgttcag
    16021 gcgaaagcgt cgaagtgcgc gaccggctgg cgctcgacgg taaaaaagca ggcggctatt
    16081 ttaaccagca gattgtcgag ccaattgcgg gtaaaaacgg accgctcggc tatctgcgcc
    16141 tgacgctcga cacccatacg ctcgccaccg aagcccaaca ggtggataac accactaaca
    16201 ttttacgcct gatgttgctg ctctcactgg caatcggcgt agtgctgacc cgcacgctgc
    16261 tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg
    16321 tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga aaggaaatcg
    16381 ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt aacctggcgg
    16441 tggaagagtg tatttttcgc caaatgcccg ccacgcagcg cgttctgttt ctctggcgca
    16501 atgccgacac ggtagtgatt ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc
    16561 ggatggaaga agataacgtc cgcctggcgc ggcgcagtag cggtggcggc gcggtgttcc
    16621 acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac gataaaacca
    16681 tctctacgtc gattgtgctc aatgcgctga acgcgctcgg cgtcagcgcc gaagcgtccg
    16741 gacgtaacga tctggtggtg aaaacagtcg aaggcgaccg caaagtctca ggctcggcct
    16801 atcgcgaaac caaagatcgt ggcttccacc acggcacttt gctgctcaat gccgacctta
    16861 gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa ggcattacct
    16921 cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggatcacc catgagcagg
    16981 tttgcgaggc cataaccaag gcctttttcg cccattatgg cgagcgtgta gaagcggaaa
    17041 tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt gcccgtcaga
    17101 gtagctggga atggaacttc ggtcaggctc cggcattctc gcatctgctg gatgaacgct
    17161 ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc acccgcgccc
    17221 aggtgtttac cgacagcctc aaccccgcgc cgctggaagc cctcgccggg cgactgcaag
    17281 gctgcctgta ccgcgcggat atgctgcaac aagagtgcga agcgctgttg gttgacttcc
    17341 cggaccagga aaaagagcta cgggagttgt cgacgtggat agcgggggcg gtaaggtaac
    17401 tcgctgatta aaataccaaa ggaagggaat atgatcgatc tggaaaacca ggagcgggag
    17461 attatcaatt taatgttaag ccagcgtatt tcctggctag ccgccgtgcg tatcaggcat
    17521 aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc tctaaaatag
    17581 atcgaaaaga cggaacggtt tagctcaaac atcaaaagca aaatggcaga aatttatggt
    17641 tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa atgaatagat
    17701 tcgtgaatag atacttgaat agattttatt ttctatccat atgaaaaata atagtaaatc
    17761 tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtct cccactccgg
    17821 cttcacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc agcagaacgg
    17881 attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg gcagtggtct
    17941 gctcgtgagt gcggatgtgg tcagacacgg tacagatggt cagggctttc gcgccaaatt
    18001 ctgcagcgac gccgtagata ccagccgctt ccatttccac gccgaggatg ccgtattttt
    18061 ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag aacaggttac
    18121 caacgcgagc atcaatgccc agtgctttag ccgcgtctac cgcgttacgc accatgtcaa
    18181 agtcagcgat agcggcaaag tcatgatctt taaaacggat gcggttaact ttggaatcgg
    18241 tgcaggcacc cataccgata acgacgtcgc gcagttttac gtgcggcaga actgcgccac
    18301 aggaacccac gcggataatt ttcttcacgc cgaaatcggt gatcagttct ttggtgtaga
    18361 tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg cctttgtaag
    18421 taccggtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct tcaaggaaag
    18481 tttcagcaat atacttcgca cgcagcgggt cgcctggcat caaaactacg tcagcgaaat
    18541 cgcccatttc tgcattaatg tgtggggtag ccattgtttt atccttatca acatgttttc
    18601 accctctccc tgagggagag ggccggggtg agggcatcaa gccgcaccca ttatcagaac
    18661 atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt ctggccgata
    18721 tccgcgaagg tttcacggtg acccagtgag cccggtttta ctttcgggcc gtaaaccagt
    18781 accggaatgt gttcacgcgt gtggtcagta ccggtccagg tcggatcaca accgtggtca
    18841 gcagtgagga tcaggatgtc gtcatcgcgc agcagagaca tcagctccgg cagacggcgg
    18901 tcgaacagct ccagacccgc agcataaccg gcgacgtcgc gacggtggcc ccaggaagag
    18961 tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt catctctttg
    19021 atggtggcgt caaacagcgc gtccaggcca gttgctttca ctttcttggt gataccgcag
    19081 ttggcgtaga tgtctgcaat tttaccgaca gaaaccacct gaccgtgttt ttcatcaacc
    19141 agtttctgca gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg gttaccggta
    19201 cgctggaagt taccggcttt gtcaccgata aacggacgag cgataacacg accgatgttg
    19261 tagccgccgt tggtcagctc ttcacgggcg atttcgcaca gttcgtagag tttatccagg
    19321 ccaaaggttt cttcgtggca ggcaatctgg aacacagagt cagcggaggt atagaaaatc
    19381 ggcttgccgg ttttcatgtg ctcttcgccc agctgatcca gaatgaccgt accggaagag
    19441 tggcagttac cgaggtaacc cggcagatta gcgcgttcaa ccagtttatc cagcagctct
    19501 tgcgggaagc tgttttcgtg atcggagaaa tatccccact caaatagaac cgggacaccg
    19561 gcgatttccc agtgaccaga cggggtatct ttaccggatg acatttcgtg cgcccatgcg
    19621 tacgcgccga taacttcagc gttgccgtcc attcccgccg gaatgaaacc ggtagaacct
    19681 tcgtgtgctt tcgccagccc cagacgggtc agatttggca gattgagcgg acctttacga
    19741 ccgttatcag cttcgccttt ggcacaagct tctgcgatat gacccagggt gtcagccccg
    19801 acgtcaccaa agcgttctgc atcttctgta gcgccgatgc cgaatgagtc cagcaccata
    19861 ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat gatcagatca
    19921 gtataccgtt attcgctgat acggcgatag acagttggtg tgctttccgg tgctttatcg
    19981 gcaagtttaa ttgccgcttt caccgctttc gccgcttcct gccagctgtt ttcgtctttc
    20041 gcgtggataa ccgccagcgg acgctgaccg tctacctggt cgcccaaacg cgccatatca
    20101 gtaaagccga cgctgtaatc aatggtgtca gatgcctgac gacgaccgcc gcccattgca
    20161 accactgcca tccccagcgc gcgggtatcc atttcactga caaaaccttc ggtatcagca
    20221 tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc aacgaagtcg
    20281 gttgggcctt tttgtgccgc taccatacga ccaaagactt ctgccgcttt accgttgtcc
    20341 agcactgcct gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt gccggagata
    20401 agcatctcca cgcacagcgc catcgtgaca tcaaacagac gcgggttacg atactcaccc
    20461 gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga ggccagtacc
    20521 tgattcatgt cggtgagcag cgcggtggtg cgcacgccag cgccgttagc cacgccaaca
    20581 atcgcttcgg caagggcttc agagagttcg taggtcggca taaacgcgcc gctacccact
    20641 ttcacgtcca tcaccagcgc gtccagacct tccgccagtt tcttcgccag aatagaggcg
    20701 gtgatcagcg ggatggagtc cacggttgcg gtaatatcac gggtcgcgta gaaacgttta
    20761 tccgccggag ccagtgagct ggtctgaccg ataatcgcca cgccgacgtc tttaataatt
    20821 tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc cagtttgtcg
    20881 agcgtaccgc cagtatgacc gaggccgcga ccagagatca tcggaatata gccgccgcag
    20941 gctgcgacca tcggccccaa catcagcgaa gtcacatcgc cgacgccacc agtggaatgt
    21001 ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt tcctgaatct
    21061 cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa gaaaatggtc
    21121 atcgcgaggg cggcaatctg cccttcggag atagtgttgt cgcgaatgcc gttgataaag
    21181 aaacgaattt cttcatcgct cagtgcatga ccatcacgtt ttttacgaat aatttcttgt
    21241 gcgagaaaca aggtaacctc ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc
    21301 tacgccttat ccggcctacg ggtagatacg tctcgtaggc ctgataagcg cagcgcatca
    21361 ggcgtaaagc atcttactta gtagctgctg gcgctcttac cgtcaccgtg gcccagcgct
    21421 ttcaacaggc ttgccagcag gctggaagca ccaaagcggt agtgacgcgc atctgcccag
    21481 tcagcaccga acagttcatc tgcgatggcg agatatttct gcgcatcttc cgcagtacgc
    21541 acgccgcccg ctggtttgaa accaacggtt ttttctacgc ccatatcacg gatcacttcc
    21601 atcatgatgc gcgcgctttc cggcgtcgcg ttcacagcca ctttaccggt agaggttttg
    21661 atgaagtccg cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct
    21721 ttcagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct
    21781 ttacaggctt tcaccaggtc aaaaccaacc tgctcgttac ccgccatcag cgcgcggtac
    21841 gggaacacca cgtcaacttc atcggcaccg taggcgattg ccgcacgggt ttctgccagc
    21901 gcgatttcga tgtcatcgtt accgtgtggg aagttggtta ccgtagcaat acggatttcc
    21961 ggggtgccct gctctttcag tgttttgcga gcaatcggga taaagcgagg atagatacag
    22021 atagcggcgg tattgccgac cggggttttg gcctgatgac acagagcaat tactttctcg
    22081 tcggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt
    22141 gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt
    22201 ttgttagtat tctaacatct actccgcaac acacttcgat atacatcaca attaaggaaa
    22261 tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg
    22321 aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa
    22381 tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact
    22441 gcaacaacgt tgccagcaaa ttgtcaccag cccagtgctt agcccggagc agaagcgcca
    22501 ttttctggca ctggaagcag aaaacaatct gccttaccca cagctgcctg ccgaagcccg
    22561 ccgcgcgctg gatgaaggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc
    22621 gcgctatgtc ttacccgatt acgcccgttt tctggcgaac ggttccgaat ggctggagct
    22681 ggaaggcgcg aaagatcttg atgacgcact ctctctgctg accattcttt accaccacgt
    22741 accgtcggtc acatcgatgc cggtctacct ggggcaactg gatgcgttgt tgcaaccgta
    22801 tgttagaatt ctaacacaag acgagatcga tgttcgaata aaacgtttct ggcgttacct
    22861 cgacagaacc ctgccagacg cctttatgca cgccaatatc ggcccgtctg attcgcccat
    22921 tacccgtgca atcttacgtg cagacgcaga gttgaagcag gtttcaccga acctgacctt
    22981 tatctacgat cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg
    23041 tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg
    23101 gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct
    23161 ggtacgcctt aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac
    23221 gcgcactcta ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt
    23281 cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa
    23341 ccctgaacgt tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct
    23401 gtgtgagaaa gaagggattg ccgcacgtta cggtaaagaa gccgccgcaa atgaagtggg
    23461 ttatcgcatc agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggatg
    23521 gcaaaaacgc gccatgttac acgcacagtc ggggatcagt tccgatattg gcaccacgcc
    23581 gggcgcgcgt ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc
    23641 cccgcatcat gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat
    23701 caaacgtaac ccgcaggcac tggtacagct ttgcctcggt gcctttaaag ccggaatgcg
    23761 tgaatttacc gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtacg
    23821 tttgtcggac ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg
    23881 cgaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga
    23941 acagcagatg cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca
    24001 gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt
    24061 ggacgatggg acgctgcaat gattgcggag agtgcgtgcc acagtgtccg catcaggcgt
    24121 tgcagattgt tgacggcaaa gtgctgtgga acgctgtggt ctgcgagcag tgtgatacct
    24181 gcctgaagat gtgtccgcaa catgccacgc caatggcgca atccatgagc gtggacgaag
    24241 tgcttagcca tgtccgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg
    24301 aagccacgac ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc
    24361 aactgcgcca cctcacctgc ctggtggaca gtaacggcat gttgagcgaa accggctggg
    24421 aaaaattact cccggtgtgt gacggcgcaa tgctcgatct caaagcgtgg gggagcgaat
    24481 gtcatcaaca cttaaccgga cgcgataatc agcagattaa gcgcagcatc tgtttgcttg
    24541 cagagcgcgg caagctggcg gaactgcgtt tgctggtaat tccagaccag gtggattatt
    24601 tgcatcacat cgatgagctg gcgacgttta tcaagagact tggcgatgtt ccggttcgcc
    24661 tgaatgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc
    24721 cggaagacgt tgagccgttg gctgatgcgt taaaggtgcg cggggtgagc cggttgatat
    24781 ttccggcgct ctatttgtga catgccggat acggcctacg gttcggcaca gacttgtagg
    24841 catgataaga cgcgacaagc gtcgcatcag gcattatgcg ccgactgccg gatgcggcgt
    24901 gaacgcctta tccggcctac ggttcggcac aaacctgtag gcatgataag acgcgtcaag
    24961 cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta
    25021 cggttcggca caaacctgta ggcatgataa gacgcgttaa gcgtcgcatc aggcattatg
    25081 cgccgactgc cggatgctgc ctacgaaggg taaactaccc actaacgcta aacaacgcat
    25141 acgtattatt aagcaacact tcggcaatct catcttctgg ttccgggcgc aactcgcaaa
    25201 gcacgtcaaa cacccgcaca gcctgttccg gtcggttagg ctgcccctga aagccgttga
    25261 gcggcatatc cggcgcgtcg gtttccagca acaacgatgc cagcggtaat tttgcgatga
    25321 catcgcgggt tttactggcg cgtggatagg tgatagtacc gcctacgccg attttgtagc
    25381 ccagctgtac aaaccgctcg gcctgttgca ggctgccgga aaaaccgtga accacgccag
    25441 tgcacggtaa atcgtgacgt ttaagatgca tcgccagttt gtcgtgtgta cgccgggaat
    25501 gaaggatcac cggcagatcg tagcgtttcg ccagtttcag ctgttcgtcg agtaaccact
    25561 gctgcctctc aaattgcgga tcgtcgccga agagatccag accgatctcc cccaccgcca
    25621 ccaccttcgc cggacgcctt tccagcgcct gctgtagctg atcaagagac acatcgctat
    25681 gtttttccaa catccccgga tgcaacccca atgcggcata cagcggttga tatttttccg
    25741 ctaacgcctg cacgcgggca aaattttccg cctctgtcgc cggaacaatg atcttgccta
    25801 cgcccgcttg tgccgcgcgt tgcaggctgg cctcttcatc gccactaaac ggcgggaaat
    25861 caaaatggca gtgggtatcg ataaaacggc aaatcaagcc agatcctcat tgttaaatgt
    25921 cgggtcgttc gcctgcggag catcgctcac ttcggcaacc agcgtatcgt tggcgactgg
    25981 cgcaggcgga ataacgatcg attccggcgt caccactggc accagatggc gggtaagcgg
    26041 cgctttttca gttaatagct tgccaaccgt ggcgaggaaa taacgaccgc ataaacgccc
    26101 aagtttatag tcttcacgca gcgccggaat ccgactgcca agcgcgatac tatgtaatgg
    26161 tttcggtgga taaatttcga atatccgcag cttgcccggt ggtttctcaa taaattgctg
    26221 aatgtcacga tagctggttt catgatgctg caccagattg accagcggct gcaggctact
    26281 gtcacccagc cagcgttcca tgcgtttgaa ccactgcggc gtgtagtaca tttgtgacgg
    26341 cacagtgcgg atgacgacca acgttttagc gccctgcctt gccgcctctt taaccggaat
    26401 cgcatcactg atcccgccat ccaagtagtt aatgccttcc agcgacactc cgctacgata
    26461 aaagccaggt atcgcactgg aggcgcgaat cacatccagc cagttttgtt tggttggtaa
    26521 aaagtaatta ggcgcgtagt catcctgacg acaggcgcac atataaaacg atttgccgct
    26581 gtcaaacaac cgcgcggcgg tatccatttg caacggcatc tggcttgcgg tggcctccac
    26641 cagccagtcg agatcgataa gatttcctcc acggacaaag cgcaatggat cgaaaaattc
    26701 gcgttttgtg gtatagcgca tgatgacttt gcgcgcgtaa ccgggctgat tgcagataaa
    26761 cgccgagagg ttctgcgccc cggcagatgt gccgagataa agatcgaaag gattaaactg
    26821 cgcgcgcata aactcatcca gcacgccagc cgtgaaaatt ccacgctgtc cgccgccttc
    26881 gcacaccaga gctattcgtc caggctggaa cggccttagc gacaacggcg caatattacc
    26941 aagcgttaca ggtattcgct gccccacctc tgccttcctg tttttgttgt gaaagaatga
    27001 caaagtaacg caatttatta ttcgctaaaa ccgcaaaagc cagtccgctg gactggcttt
    27061 ggataaaatt ttcttaatgg tctgaaatct agggtcgttt tcggcccatg aacaaactca
    27121 ccaggaacag aataatcccg acgacaaaga caattttagc tgcgcctgca gcggtaccgg
    27181 ccagaccacc aaacccaagt gcggcggcga ttaacgcgat aaccagaaat atgatgcccc
    27241 aacgaaacat aagcgtctcc tttaccatag ttaatgtcac cgcttgatat gagcgtgaaa
    27301 atcactcaat gcgatatttt tagtgtggtg cacatcgcgc ctcccgacaa agttcgggag
    27361 gacgaattac gacgaattac ttagttttca gatcattttt aacgcttttc acaccatcta
    27421 ccgctttggc gatactttca gcacggtcgc tctgtgcctg ggaatcgacg gtaccggaga
    27481 gctgaaccac gccgtcggtg gtttcaactt tcacatgacg ggaagggacg atatcgtccg
    27541 ccagcagttt ggctttgatt tcactggtgg tggcggtgtc acccgcgtag cccttcacag
    27601 agccttcttt agcgtcgcga acgtgcagtt tgtcgctgac agaggtcacc ccttcaacgc
    27661 ctttcgccac tttcactgcc tcttcggcct gggcctggct ttcaacgaaa ccgctcaggg
    27721 tcacgacttt ttgatcggtt tttacagaga tatcggtgct cttgatgttg tcatgatcca
    27781 ccagggccgc cttcactttc gcggtgatgg cgctgtcatc catgaaatta ccgactttat
    27841 tcatagagct atcgactttt tgccctgcgc tttcattggt agtctgtgcg ttgttttccg
    27901 cgtaggcaga gccggtcgcg acggcagagg tcaacattac agccagcaga gttttcgaaa
    27961 tcttcagtct tgtcatagtc atcgatttat tcctgtatgt ttgctcgtaa tttgagcctg
    28021 gcaacacgag gttgcattgc tgaataggga gagacttcac cctctacaga agtcaatggt
    28081 cgccatcaca aaagcgatgg gtgatgaata acgaccatta cagcctctga atcagttatt
    28141 aatatcggca gaatgacaat cgacggcttt agatactgat atctacgcat tgaacggtat
    28201 ttaacgccgt cagaaatgtc attactttgt taaatataga tcacaatttt gaaaccgctc
    28261 gggatatcag cgaaaacata agcaaaagtg aatgttttaa gaacattccg taagcggcta
    28321 ataaggaagg gaaattgaca gggcgaggcg gttgccgcgc cctggaggca agaattaatg
    28381 ctcgcgggtc tggtggaact gaacgtccgg ataacgttcc tgtgccaggc gcaggttaac
    28441 catgctggta gcgatgtaag cgagattatc gccgccatca agcgccagtt ggctttcgtt
    28501 cttacgcttg aactcttcaa atttcttcgc gtccgcacat tctacccacc gggcggtggc
    28561 aacgttgact gattcatata ctgcttcaac gttgtattcg ctcttcaggc gcgataccac
    28621 tacatcaaac tgcagcacac caaccgcacc aacgatcagg tcgttgttgg agatcggacg
    28681 gaacacctgc accgcgccct cttcggaaag ctgtaccagc cctttgagca gctgtttttg
    28741 cttcagcgga tctttcaggc ggatacgacg gaacagttct ggtgcgaagt tcggaatacc
    28801 ggtgaacttc atcatctcac cctgggtaaa ggtgtcgccg atctgaatgg tgccgtggtt
    28861 gtgcaggccg aggatatcgc caggatacgc ttcttcaacg tgcgaacggt cacccgccat
    28921 aaaggtcagc gcgtcagaga tcaccacgtc tttcgcggtg cgcacctggc gcagcttcat
    28981 gcctttttca tatttaccgg ataccacgcg cataaacgcc acgcggtcgc ggtgtttcgg
    29041 gtccatgttg gcctgaattt taaatacgaa gccggtaaat ttgtcttcgc tcgcttgtac
    29101 ggtacgggta tcagtctgac gcggcatcgg cgcaggtgcc cactccacca ggccatccaa
    29161 catatgatcg acgccgaagt tacccagcgc agtaccgaag aataccggag tgatttcgcc
    29221 cgcaaggaac agctctttgt cgaactcgtt agacgcgcct ttaaccagtt ccagttcgtc
    29281 acgcagctgc tgtgccagat cttcaccaac cgcagcatcg agatccgggt tattcagccc
    29341 tttaacaatg cggacttcct gaatggtgtg ccctttaccg ctctgataga gataggtttc
    29401 atctttataa aggtggtaaa cgcctttaaa cagcttgccg cagccaattg gccaggtgat
    29461 cggtgcgcag ccaattttca gctcgttctc aacttcatcg agcaattcca tcgggtcgcg
    29521 gatatcacgg tcaagtttgt tcataaaggt gaggatcggc gtgtcgcgca gacgggtaac
    29581 ttccatcagc ttacgggtac gatcttcaac accttttgcg gcgtcgataa ccatcaggca
    29641 gcagtccacc gccgtcaggg tacgataggt atcttccgag aagtcttcat gccccggggt
    29701 gtcgagcagg ttaaccaggc aatcgtgata cggaaactgc atcacagacg tagtaatgga
    29761 gatcccacgc tgcttttcca tctccatcca gtccgactta gcgtgctggt tggaaccacg
    29821 gccttttact gtaccggcgg tctgaatggc ctgtccgaac agcagcacct tctcggtgat
    29881 ggtagtctta ccggcgtccg ggtgagaaat aatggcaaaa gtgcggcgct tcgccacctc
    29941 ttgcaaataa ggagacaacg tcataattct ttcttcttga gtaagcggca tcgcgccgcg
    30001 cttgttgaaa acgaaaaatt gcggctattt tacccatcaa cgggggggag gcaatcagtg
    30061 tttacacagg agctgctcca gttcatgcaa cgaagaaacg gtccaggtgg gcgcgatgcc
    30121 ttctggttgc tcgcgatggt gtgcattcag ccagcaggtc gcaagcccgg cgttgatgcc
    30181 accgagaata tcggactcgg cagtgtcgcc aaccatcagc acgcgtgaac ggtcaggatt
    30241 gcccgcctgt tccagcgcat aatcgaaaat tttcttattc ggtttagcaa cgccaacttc
    30301 ttcggaaatc accagcaaat cgaagtaatc acgcaggccc gtccgttcca gacgcacttg
    30361 ctgcaaggcg ctaaaaccgt tggtgatgat gccgatttta gcgttgccac gaatggcgtt
    30421 aagcagagaa accgcgcccg gcaacggcgt acagatttcc gccatcgcat taataaaggc
    30481 atcgttaagc aagcctgctt caactttcag ccgttcggcc cagctctcaa accgcccgtg
    30541 ctgaagctgt aatgaagtga tcgcgccgtt ttgataatcc acccacagtg gcttgttaac
    30601 ggcctgatag tcctgaaaat cttcagcggt aaaggtgacg ctgtaatcaa gaaacatccg
    30661 ctgcaggccg gtgaatgagt caaaggtaaa cagcgtttca tcggcatcaa agaaaatcca
    30721 gtcccacttc attattccac cttgtcttac atactgattg gcaacgccat gatgatggcg
    30781 tcttcgcgac cgtccgtggt ggggtagtaa ttgcggcgaa tcgtcgcctc gttaaagcct
    30841 aaactttcgt acagggcaat ggcggcagcg tttgaagcac ggacttccag ccatagtgtc
    30901 gccacgccgc gtttttccag ttcgtcgatc agatgttcca gcagcaccct tcccaatccc
    30961 tgacgctgat agtcaggatc gaccgcaata ttgaacaatg tagcttcatc cagcaccact
    31021 tgcgtaatcg caaacgccgc cattttgccg ttttgcgtta actgaaagtt gagataacgc
    31081 tcgccctggt tgctggcaaa ggttttttca ctccacggaa aggcgtgggc gcgttgttca
    31141 atgtggtaag ccgctggtaa atcagtcgtt tcgagggaag aaatcgtgtt catatgtgca
    31201 aatctgttgc cataacgcgg cgcgtgccgt tgggtttgcc cgtaattcgg tgagcgccgg
    31261 tgatgccacc tgagcgcctt ccagtgatag cggttcgtca gtacccaacc gccagctgtt
    31321 gcagcgactg ccttgcggca gcatcgcgat tttttctggc gtcagttgca gcacttgatc
    31381 ggggctgacg gttaatgcgc gcagaacatc gctcactaaa ggatcggtca gggcgggaag
    31441 atcgtttgcc accatcacca gacggacgtg tgccgggatc gcaatggcaa tctccccctg
    31501 caacgcgcca gggcgacgca gcgaccactg ggtaatgccc agttgctgta actgccagtc
    31561 tcgtcgggat gtcatagcga atcgctcctg ttgtcagggg cgcaaatata gcaaattcgt
    31621 cgataccgcg ccaacatatg gctataatcg ccgccagtat caattgagga gcattccatg
    31681 tctgcattta ccccggcaag tgaagtcttg ctgcgtcaca gtgatgattt cgaacaaagc
    31741 cgtattctgt ttgccggaga cttacaggat gacctgcccg cgcgtttaga taccgcggcc
    31801 agccgtgctc atacccagca attccaccac tggcaggtat taagccgcca gatgggggat
    31861 aacgcccgtt tcagtctggt cgccacggtg gatgacgtcg cagattgcga tacgctgatt
    31921 tactactggc cgaagaacaa accggaagcc cagttccagt tgatgaattt actttctctg
    31981 ctgccagtgg ggacagatat ttttgtcgtt ggcgagaacc gcagcggcgt gcgcagcgcc
    32041 gagcagatgc tggcagatta tgcgccgttg aataaagtcg acagcgctcg tcgctgtggc
    32101 ctctattttg gtcgtctgga aaaacagccg gtatttgatg ccgataaatt ctggggcgaa
    32161 tacagcgtcg atggcctgac ggtcaaaacg ctgcctggcg tgtttagccg cgacggtctg
    32221 gatgtcggta gccagttgct gctctcgacg ttaaccccgc acacgaaagg taaagtgctg
    32281 gatgtcggct gtggcgcggg tgtgctttca gttgcctttg cgcgccattc gccgaaaatt
    32341 cgtctcacct tgtgcgatgt ctctgcgcca gcggtagaag ccagccgcgc aacacttgcg
    32401 gccaacggtg ttgaaggtga agtctttgcc agcaacgtct tttccgaggt gaaaggtcgt
    32461 tttgatatga tcatctccaa cccgccgttc cacgatggga tgcaaaccag cctggatgcg
    32521 gcgcaaacgc tgattcgcgg cgcggtgcgt catcttaata gcggcggcga gctgcgaatt
    32581 gtagcgaacg ccttcctgcc ttacccggac gtgctggatg agacatttgg cttccatgaa
    32641 gtgattgcgc aaaccgggcg tttcaaggtg tatcgcgcca ttatgacccg ccaggcgaag
    32701 aaaggttaat tatctcgccg aataccgtgt cggatgcggc gtgaacgcct tatccgacct
    32761 acaaaacctg cacgttagcc cttcgtaggc cagataagac gcgccagcgt cgcatctggc
    32821 atctgccccc gcatgcctac caaacctgca cgctagtttc ctgatggaca tttttccagc
    32881 aattacacct ccggcgataa ttaactattg acgaaaagct gaaaaccact agaatgcgcc
    32941 tccgtggtag caattctttt taagaattga tggtatgcga aggtggcgga attggtagac
    33001 gcgctagctt caggtgttag tgtccttacg gacgtggggg ttcaagtccc ccccctcgca
    33061 ccaaaaacca cgttgatatt gctcgcactg ggcgaaggtg gcggaattgg tagacgcgct
    33121 agcttcaggt gttagtgttc ttacggacgt gggggttcaa gtcccccccc tcgcaccaaa
    33181 cgaggcgata tcaaaaaaag taagatgact gtgcgaaggt ggcggaattg gtagacgcgc
    33241 tagcttcagg tgttagtgtc cttacggacg tgggggttca agtccccccc ctcgcaccaa
    33301 ttatctttac ttcctttctt gtttcttcct tgatttttca tccctgattc aaattcatta
    33361 tcatcagcat gatgccagca ataagcgcca ggcacgatgg tagcaaaacg taatgccgta
    33421 actgtttgtg atacagcatc accggcgtca atactaatcc catcagaatg acaatccgcc
    33481 actgatccgg cgataaatcc gcatacatca gccctgcaat cagcaatcct ggcagcaaca
    33541 ctccccagcc actgcccagc atacgttgca acatggtttc atctctttca ttgataatga
    33601 taaccaatat catatgataa tttttatcat ttgcaagcca gagaaatccc ttgctatcgg
    33661 gtaaacctat cgctaggatt agcaatcatt atcatttaga ttactatccc gattatggcc
    33721 tatcgttccg caccgctcta tgaagatatc atctggcgaa cgcatctcca gccgcaggat
    33781 gctgggcttg cacaagccgt acgtgcgacg atcgccaaac atcgtgaaca tttgctcgag
    33841 tttatccgcc tggatgaacc cgctccgctt aacgccatga cgctggcgca atggtcatca
    33901 ccgaatgcgc taagttctct actcgcggtc tattccgatc atatctatcg caaccaacca
    33961 acgatgatcc gcgagaacaa accgctgatc tcactatggg cacaatggta tatcggcctg
    34021 atggtgccac cattaatgct ggcgctactg acgcaggaaa aagcgttaga tgtgtcgccg
    34081 gaacatttcc atgctgagtt tcacgaaacc ggacgcgtcg cctgtttctg ggtcgatgtg
    34141 tgtgaagata aaaacgcaac accacattcg ccgcagcagc gaatggaaac gttaatcagc
    34201 caggcgctgg ttccggttgt gcaagcacta gaagcgaccg gagagatcaa cggcaaactt
    34261 atctggagta ataccggtta tttgatcaac tggtatctca ctgagatgaa gcaactgctc
    34321 ggcgaggcga ctgttgaatc gctgcgccat gccctctttt ttgagaaaac gctcacgaac
    34381 ggtgaagata atccactctg gcgtaccgtg gtactgcgcg acggcctgct ggtgcgccgc
    34441 acttgctgcc agcgttatcg cttaccggat gtgcagcaat gtggcgattg tacgctgaaa
    34501 tgaaagcact gccggggaag taaacccggc atcatgcgga ttaataggga tgcaacacat
    34561 tacttgtttc gcaatgccgc atacataaca gaatatctgc ggcctccaac aaccctgcgt
    34621 cagaactgac gcccagtttc gacatcacat taaatttatg tgcacggatc gttttgatat
    34681 tgcgtttaag ctgctcggca atttgtgtca ttgagtagcc acgcgacata aagcgcaata
    34741 tttctctctc cgtcgggctt aacgtccggc tttggtttat gtaccactga ttgttcagtc
    34801 ggtcggtcgc ctgacgtaca ccatttaatg acaaaaagag ttcctgatga aaaatctcca
    34861 gcgttgacgc tttacttaat acaccgtcca gcggtgatgg cgacaatgaa ccaatcagcc
    34921 gagcttcaat atcatcatcc gcaataacta aacgccgggt acgcggaaac ttaatcgcca
    34981 gttcagtcag gcaagataat ccctctctgc gctcacttct catggcagaa aaagaaaaaa
    35041 tgaccgccgc aaacgagatc cgcgacatgg catcctgaaa tgactcctga ctggtatagg
    35101 tatggagctt ataagggtta cccgcaaact ttctgaataa actctcaata ccgatactac
    35161 tcatgacgca tttttctatg agtgcgacat ttctcttctt aattcggctg tgttccattc
    35221 tgcggcatct ccataaacag aaggcaagtt aagttctctt gcaccctgcg cgctatttat
    35281 ccatgcatac atttcagcgt tgctgtgaat tgccagtcga cgcatcgcac tgtttttttg
    35341 ggcgctgata gttttattac ttttcttaag caatgaagct atctggttga tgccccatcc
    35401 tttacctaat agccgcagta ccttgcgctc tgaaagagtg agaatgacgg agcgatagct
    35461 aaaatcatga gtttcagtca ttgccgggga ggtcagcatg gcgctgatac gctctgcgtg
    35521 cgtattgccg gaacgtacgg tcttgaccag attctcaatg ggttcaacat cagacaataa
    35581 cgtcgccgta gggcacatga gcagttctac tgcctgggaa taccaggagc gtgacaccat
    35641 gaaaacccag tgaatttccc gatattgtga gattaaagaa taataatgtt cacaaacaga
    35701 acgtggatct tcactttcac cggctaaatc agcaatgact aatccggaac gacgtaattg
    35761 taataaggta aggtcctccg cagaggcgct ggatattatt tcatattcag ggaagtgagt
    35821 cctcatgacc tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt
    35881 tttgcagcat cctggcaaca tatttcctcc acatccactg tctaaaaatt atgatggttt
    35941 attcaggata ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta
    36001 tccttagcat tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg
    36061 tcagaaggtt agcatcaaat gataacctgg tgaaatagcc tggagacatt taaaatatct
    36121 acattaagtt gttaggaata cttaatgaaa aacagaaatc ac
//
LOCUS       DAFNBJ010000030        35753 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000030 DAFNBJ010000000
VERSION     DAFNBJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.030"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(1..840)
                     /locus_tag="L2X49_001465"
                     /pseudo
     CDS             complement(1..840)
                     /locus_tag="L2X49_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1465..1890)
                     /gene="arsC"
                     /locus_tag="L2X49_001466"
     CDS             complement(1465..1890)
                     /gene="arsC"
                     /locus_tag="L2X49_001466"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBN4796572.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTDERLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1903..3192)
                     /gene="arsB"
                     /locus_tag="L2X49_001467"
     CDS             complement(1903..3192)
                     /gene="arsB"
                     /locus_tag="L2X49_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBN4796573.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLVALALRLSFTL"
     gene            complement(3246..3599)
                     /gene="arsR"
                     /locus_tag="L2X49_001468"
     CDS             complement(3246..3599)
                     /gene="arsR"
                     /locus_tag="L2X49_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBN4796574.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4339..4422
                     /locus_tag="L2X49_001469"
     CDS             4339..4422
                     /locus_tag="L2X49_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBN4796575.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4476..5828)
                     /gene="gorA"
                     /locus_tag="L2X49_001470"
     CDS             complement(4476..5828)
                     /gene="gorA"
                     /locus_tag="L2X49_001470"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBN4796576.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5900..6742)
                     /gene="rlmJ"
                     /locus_tag="L2X49_001471"
     CDS             complement(5900..6742)
                     /gene="rlmJ"
                     /locus_tag="L2X49_001471"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBN4796577.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6945..8987
                     /gene="prlC"
                     /locus_tag="L2X49_001472"
     CDS             6945..8987
                     /gene="prlC"
                     /locus_tag="L2X49_001472"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBN4796578.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8995..9747
                     /gene="rsmJ"
                     /locus_tag="L2X49_001473"
     CDS             8995..9747
                     /gene="rsmJ"
                     /locus_tag="L2X49_001473"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBN4796579.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9796..11265)
                     /gene="dtpB"
                     /locus_tag="L2X49_001474"
     CDS             complement(9796..11265)
                     /gene="dtpB"
                     /locus_tag="L2X49_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HBN4796580.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(11583..12017)
                     /gene="uspA"
                     /locus_tag="L2X49_001475"
     CDS             complement(11583..12017)
                     /gene="uspA"
                     /locus_tag="L2X49_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBN4796581.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12408..12743
                     /gene="uspB"
                     /locus_tag="L2X49_001476"
     CDS             12408..12743
                     /gene="uspB"
                     /locus_tag="L2X49_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBN4796582.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12814..14313)
                     /gene="pitA"
                     /locus_tag="L2X49_001477"
     CDS             complement(12814..14313)
                     /gene="pitA"
                     /locus_tag="L2X49_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBN4796583.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14545..15747
                     /locus_tag="L2X49_001478"
     CDS             14545..15747
                     /locus_tag="L2X49_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBN4796584.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAVQRQ"
     gene            15854..16675
                     /locus_tag="L2X49_001479"
     CDS             15854..16675
                     /locus_tag="L2X49_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7 transposase TnsA N-terminal
                     domain-containing protein"
                     /protein_id="HBN4796585.1"
                     /translation="MGRRRKLEALADFQRALKNKYGLGEGDSYKPWLRVQDVRSRGNS
                     GKIQGIKVNREHHMLSEHESCFFYIAEFCDSVIDIREQFPLLPLDLSVKIAKTFGVDH
                     PIVPGSKSPNVMTTDFVLTRSDGVNTWYEAVSVKPAEKLTDKRIAEKLDIERNWWQLQ
                     GVPFHLFVMTKQNQIQSKNIQWVTAPVRQGRKFPTDLVESALALIFPGTMLTEEICDE
                     FVREFKIEHDDALVLLKTLIATKMVDVDLDKPIADTGILNIVKVTDFKVVRHHAS"
     gene            16665..18818
                     /locus_tag="L2X49_001480"
     CDS             16665..18818
                     /locus_tag="L2X49_001480"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBN4796586.1"
                     /translation="MQVERNSVWQVTRIDGIKDGMYRVLEHYANDDLLILFRLHDGKA
                     LEKPVPVELPLFLESAKCGNSKISSYPIPFYQLISEEEISPAHRDKRNQRFELIHDLI
                     SNPEFLLDVALNQRSKTVASHAKERKVYVQKIYRSLNQYWRYGQEINALLPAYKHCGG
                     SGKPRLSGKAKRGAPIQLSTPSMVAPSGVNTTEEDKAKFLLGMKKYGLKGKKVSLSRV
                     YEQMLKEYYADELRSAEKENREPEIPSLRSFRYWIKKLIPEAELIRKQTTAGDFERNR
                     RGLRGAATDHTEVPGSCFELDATVLDVHIVSEFNRNHVLGRPTVYCVVDKESRMMVGL
                     HVSMEYASWRAGRQALVNSFTSKKAYCARFGIEIEESEWPCHHIPQRLLCDRGEFICN
                     KPEELAVPLIGHLSIAPPYRAELKGIVEHRFQILNEKLVHELMGTTKGRHYIRGDRDP
                     RLDATLTLSEVTKLLIDQVLEHNSSIFDGLAGQTPLLVESGLPPTPLNYWNTHLKRHR
                     HALNKADEADIRARLLPVEQVSMTSKGIRLNDDMYYECDRAEFEDWKVIARSNGRWKL
                     EARIDQDNASFIYVRLRPSEGFTRCTLMTRSSSFEERHRADVLYFEDWKKVSKKRSKP
                     TSKSIERHNRRKTITANAREELKKEPALKTKAEKTLGIKERRREAIMEARISSDDSNY
                     TEPSENQSSVDKLAADRASKVVSLLKRERGSNSET"
     gene            18808..20256
                     /locus_tag="L2X49_001481"
     CDS             18808..20256
                     /locus_tag="L2X49_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN4796587.1"
                     /translation="MKLEKAKYRPAILPEHRGNPLIEALPAKHTDEELVDKLSFYPPH
                     SFEETQLAPFERVEYLTRLKDLRQPLPIYLDCFRAVEMAIKEGYSVKNPLSPTTMNYL
                     HYPTDRRPNIEPRTGYFKPKGCGITVIGESGVGKTCMLEQILGCFPDTIEHSQYKNHS
                     IPLRQVVWIKVDCPDDSSVRALCHKILSELDSKLGFEPTKRAGTIPLLLDQIEARIKS
                     SFLGILVIDEMQNLNLAKTGGADRLLGFIHNLVNNLGIPILFCANPPFNQLLSRTLKA
                     ARRAESNGYFDVVLMENDGVWELFIEELWELQWTNVRTPLTVELNDKLYELSVGNMDL
                     AVRIFREAQRYIIGCGDERITGELLEHAASIAIKASKAVTDEIRRERSITALKRKRNI
                     TSLPASADMARTQKTELVAIEGVPQSIPGDLSRPHHPEFADALTILQTADDLFERIGN
                     PDLMQHAAAEEKPLELLRQEGVFCEHPLKVFA"
     gene            20277..21695
                     /locus_tag="L2X49_001482"
     CDS             20277..21695
                     /locus_tag="L2X49_001482"
                     /inference="COORDINATES: protein
                     motif:HMM:NF018259.1,HMM:NF027308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HBN4796588.1"
                     /translation="MKLPVSLPDELLLSRLIRYVTVSGDKGDNFASKVFGSRKVSIHP
                     FLTARLEQLAGWGLESAEVMLFQQTLAPLFLFFLPTYADRLKRSMLAGDGAEAHRASQ
                     LSLFGCGNSLCLKWCPVCAQQDLRLYGVAYWHRTHQIPGVTACAFHPVLLEKLELVRR
                     QRIIAELLPTLIDQPRVAFDAEVQVARFGFKLLQLVTGGIPRVDTVLTYRSKLSELGF
                     ITAHHRVRRKSLMEDFCADIKHYRPGPDTPFPRHPHDYRYLSQLLDPHTSHHPFRHLL
                     FAYWLFREPEKMLSTKHLCAASLQPISLKSLEGEISTEQQCLQLLRKQYSLSEIYRLT
                     GKSRCYLRRLALLNDIPLKLKPKKLTGECQKRIIGLAYAGMHRKAISERCGIGIGSVE
                     QVISSQPGLVEHRKRCHWESKRRRCRLEIAKYLKSHPGALRRDCKAQCNAAFFWLYLN
                     DPIWLDSALPEPIKPAGRYPNC"
     gene            21888..22097
                     /locus_tag="L2X49_001483"
     CDS             21888..22097
                     /locus_tag="L2X49_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN4796589.1"
                     /translation="MSTEQWVTAEHVAEHLGIAKDTVYRWREHKGLPAHRVGRLWKFQ
                     LSEVDEWVRAGGAGEDSGEQGGHRA"
     gene            22165..25134
                     /locus_tag="L2X49_001484"
     CDS             22165..25134
                     /locus_tag="L2X49_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBN4796590.1"
                     /translation="MTTTEQQIELDLIAKLGDLKYTYRSDIRDRTTLEANFRAKFEAL
                     NRVHLTDSEFQRLLDGIITPDVYGAAQRLRNINSFERDDGTPLNYTLVNIRDWCKNDF
                     EVVNQLRMNTENSHHRYDVMLLINGVPVVQIELKTLAVSPRRAMQQIVDYKADPGNGY
                     SKTLLCFLQLFIVSNRTDTWYFANNNARHFSFNADERFLPVYQFASEDNKKITLLDSF
                     AEKFLAKCTLGQMISRYMVLVASEQRLLMMRPYQIYAVKAIVDCIHQNCGNGYIWHTT
                     GSGKTLTSFKASTLLKDNPDIDKCLFVVDRKDLDRQTREEFNRFQEGCVEENTNTETL
                     VRRLLSDDYADKVIVTTIQKLGLALDGANKRNYKERLEPLRNQRMVFIFDECHRSQFG
                     DNHKAIKEFFPNAQLFGFTGTPIFEKNASYQQIEGQQASYRTTDDLFQRCLHQYTITH
                     AIEDRNVLRFHVDYFKPEGKNPPKPGEGVAKPKVIETILAKHDAATNGRKFNTVLATA
                     SINDAIEYFELFAEIQKQKTEQDSEFRPLNVACVFSPPAEGNKDVQQIQEDLPQEKED
                     NQQDPDGKKAALTRIVTDYNTRFGTNHRISEFDLYYQDVQKRIKDQQYPNSDLPAAQK
                     VDITIVVDMLLTGFDSKYLNTLYVDKNLKHHGLIQAFSRTNRVLNDSKPYGNILDFRQ
                     QQKPVEEAIALFSGEKIDNPREIWLVDPAPKVIDNLQTATQKLADFMQSQGVGNAPEE
                     VANLKGDAARAQFVNLFKEVQRLNTQLDQYTDLSDEQKAQITQIAPPDKMQGFKGMYL
                     EAAKRLKEQQDRDQTPPEVQQLDFEFVLFASSVIDYDYIMGLIAKLTQQKPGKLTMNR
                     EQLIGLIQSDAKFIDEREDIAEYIRGLPVNVALDEKQIRSGFERFKAEKKARELATMA
                     EKHGLDVVALQAFVDDILRRHIFDGERLSELMAPLELGWKARTQKELALMEDLTPLLH
                     KLQGREIAGLAAYEEER"
     gene            25131..27773
                     /locus_tag="L2X49_001485"
     CDS             25131..27773
                     /locus_tag="L2X49_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004722060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HBN4796591.1"
                     /translation="MMGTVFAQGAQWVRADFHLHTRADREFKYTGDDNFYNSNYVDAL
                     EKAGIHLAVITNHNKFDFEEFKALRKTAQKKDIALLPGVELSVNDGANGIHTLVVFSD
                     DWLADGHDHINPFLGVAFEGKVPAQYEQENGRSSLSLLETIKKLESYHRDFFLVFAHV
                     EAPSGLWAELDGGRLAELGQNEFFRRRTLGFQKVRTYNKQQEKSKPCRTKTQQHLGDW
                     YPAELEGCDAKCIEDIGQGKACYLKLGELSFEAVKFALSDPASRVAMEPPKHQGSFIR
                     GIHFDGGILDGKTLHFSPELNTLIGIRGSGKSSILEAVRYALDIPRGEKAQDTKYKDE
                     LIRHTLGSGGKVTLTACDVYGQEFTISRIFREAPNVYLDGKLQPGVSIRETVLRRPIY
                     FGQKDLSSTGEGFETDLVEKLVGEKLRMLRDEIETQRQHVRDAASRWLKLSNTAELRR
                     DHESQLTDANFRLKKFEEYGVADKLQKRLGYQQDATALTRMKDRADSFVIALGQLISE
                     HEDDLRNATSYVSKQNPGFFTAYYAEFANLVAKVDQLKKIEQEARAITARLQAKQGEF
                     DDARQSLQEEFAQVERQLAQELKQTGMTAIQPDDFLTQQQRKTKAEQMLEALAKQETQ
                     QSSIRDVLFAEIDKLNGLWLREFSAIKAELDRVNAGHTALQIEADFKGDKEAAIGFMQ
                     QLFKGSNIRETTLRAVMEDYADFGGLLRDLPNALKKAGSTPEVFEKTFMQNLVEFVTC
                     QVPNRFVIRYRGKELKHHSLGQRASALLLYVLSQRQNDVIIIDQPEDDLDNQTIYDDV
                     IKLLREMKPHAQFIFATHNANFPVLGDAEQVHACRYQDEQVAVQSGSIDARPVQDAII
                     NIMEGGQEAFNRRKEVYNLWKPQS"
     gene            27755..28918
                     /locus_tag="L2X49_001486"
     CDS             27755..28918
                     /locus_tag="L2X49_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HBN4796592.1"
                     /translation="METTELIEALSRGEDSRHQFKESFTNADALAAEIVAFSNTAGGQ
                     IFIGAQNDGTVLGLSHADIDRLNQLISNSASQNVRPAVNPLTENVPHPNGTVMVVTVA
                     EGVSKPYMDKNGVIWVKNGSDKRRATSREEIQRLFQQAGLVHADETPVAGLGAGDVDL
                     PYFETFFEQQFGEALAQHKQPLPQLLTNMNLMNQGLLNVAGSLLFAKAPQYALPAFIV
                     KAVAFVGIDIEDQRYIDSRDITGKLADVFQQTLGFIVANTRATQGEQGFNSQGQAEIP
                     RIVWEELVANALIHRDYFISSPVRVMVFADRVEIISPGHLPNNLTIENIKAGNSNMRN
                     PILASFAAKLLPYRGLGSGLLRALRAWPQIELIDDRAGNLFKAIVTRPQVQAA"
     gene            28915..30192
                     /locus_tag="L2X49_001487"
     CDS             28915..30192
                     /locus_tag="L2X49_001487"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBN4796593.1"
                     /translation="MSGNEKQQLVPRLRFPEFKNTAEWQSPTLGEVSEPVEERVGERK
                     LTPVSISAGIGFVPQAEKFGRDISGSQYKLYTVVRDGDFVYNKGNSLKFPQGCVYDLQ
                     GWGEVAAPNVFICFRLKNGYENGFYRQCFERNTHGIQLRKHITSGARSNGLLNISKEA
                     FYSVAIPVPSHAEQQKIADCLSSLDALIAAEIQKLGTLKTHKKGLMQQLFPREGETVP
                     RLRFPEFRDDDGWESDLLGNIFDTTSGGTPSRSTKEYWDGYIPWITTSLVNFGIITHA
                     DEFISEAGLKNSAAKLFPRGTVLIAMYGQGKTRGQVALLGIEAATNQACAAILPRKEI
                     DPNFVFLNLASRYDEIRQLSNSGGQENLSQGLIRELSFSYPLNPAEQQKITACLTSVD
                     NVINAQVQKIETLISNKNGLTQQLFPFLGEVSS"
     gene            30189..31304
                     /locus_tag="L2X49_001488"
     CDS             30189..31304
                     /locus_tag="L2X49_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN4796594.1"
                     /translation="MKPGQTFADLLRLAAHLRGDLENKKAILLYAYNGTGKTRLSMTF
                     KDMGKQAEARDTLYFNAFTEDLFHWNNDLVGDEDRRLLLNRDSRFFQGLFDLEMDNRI
                     RPLLRRYADFDFRIDTEGPEWAVRFSRLVDGQPVDNIKVSRGEENIFIWCFFLAVVEL
                     AMDADIEAYQWVKYLYIDDPISSLDEHNAIAVANHLAQLLKRQDNRLKTVISTHHTLF
                     FNVLCNELGKARRYVVNKHSANGGYLLRPEEGDTPFFHHVAALAELYQAAQEDRLFTH
                     HFNMLRTILEKTASFHGHKNFSVCIKQDDDDPDGILHARLINILSHGNYSLYEPQQML
                     DENKAYFKKILNEFLNRYPFNPDLFPQVPEAATAGTP"
     gene            31301..32935
                     /locus_tag="L2X49_001489"
     CDS             31301..32935
                     /locus_tag="L2X49_001489"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBN4796595.1"
                     /translation="MTEFEKQKLGKILWAIADKLRGAMNADDFRDYMLAFLFLRYLSD
                     NYEAAAQKELGSDYPRQIDGSVSTPLQLWYESNYEDVPEFEKQMRRKVHYVIEPQYLW
                     GNIAEMARKQDDELLRTLQKGFKYIEEESFASTFRGLFSEINLASDKLGKTYIERNAR
                     LCKIIAEIAKGLVQFSTDSDTLGDAYEYLIGQFAAGSGKKAGEFYTPQRISDILSAIV
                     TLDSQEPATGKRSHLDSVFDFACGSGSLLLNVRRLMGPHGIGKIHGQEKNITTYNLAR
                     MNMLLHGVKDSEFDIFHGDTLFNEWDMLRETNPAKMPRFDAVVANPPFSYRWEPSEAL
                     GEDVRFKNYGLAPKSAADFAFLLHGFHFLKQDGVMAIILPHGVLFRGGTEARIRTKLL
                     KDGHIDTVIGLPANLFFSTGIPVCILVLKKCKKPDDVLFINAAEHYEKGKRQSQLLRT
                     DEMTDGGIGHIEKIIDTYRYRKEEPRYSRRVSMDEIEKNDFNLNISRYVSTVQAEEEI
                     DLAAVHTDLLVVEKQIGAAKERYNQYLAELGLPLLP"
     gene            33115..34317
                     /locus_tag="L2X49_001490"
     CDS             33115..34317
                     /locus_tag="L2X49_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796596.1"
                     /translation="MNQASGICPQSDESLQSFIHRMMLRVGWSDFTTLITHGGWGSNP
                     SVPYEVKQEFDAFDASILLDLFENQYYANKNYSLFDGRFSHISLFQETFFPHKKKTFC
                     GSRVPLRFCRACIEKQLHERGFSYFKLDWLYEAFCEIHATPLLKVKESLSLSKIKNTL
                     KFVFLADWHEILDVVSEEVLLEDEHHKIEESDLTRYNRMLSFSPCAKRGLVEYFMSSS
                     DYYPTGCSSIADYGFLSKIQRDLLFKKRKRDSLQSHLEEAYEYELSNNYHSLIEHCIK
                     DLEIMAIHHFGKGHWVIKSTSRKCVDCLRHNVDDFRSCPAAGIISCTGGYLSSAENQC
                     DKSLDSLESDVELHQRRLKVTDGERFVRDSIEKSRAITAAGGPQAYRREREARLKAAA
                     KALDPLIS"
     gene            34384..34551
                     /locus_tag="L2X49_001491"
     CDS             34384..34551
                     /locus_tag="L2X49_001491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796597.1"
                     /translation="MARTLEELLQQEKPEVVEAAKDKAEKILRELEGVDQDTPQDELD
                     EPADNHSSGSS"
     gene            34775..35617
                     /locus_tag="L2X49_001492"
     CDS             34775..35617
                     /locus_tag="L2X49_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7 transposase TnsA N-terminal
                     domain-containing protein"
                     /protein_id="HBN4796598.1"
                     /translation="MARGRRLKSYLDYENALGDGIGVGYGQSYQPWLRAQDVKSRGNR
                     SIVFGLKTFRNHHLLSSVESNFFYLAEFNDSVIDIREQFPLFPLRLTQQIANHLHFQH
                     PMVRGVRGVPVEVLNVMTTDFLLTLRTPEGGLRYKAIAVKHNESIPEREAQKLEIERM
                     FWQLIDVEFQIYVGSELNNVVGKNICWATSVLRDGSEFYDKYPLDKILWKLKPDVYPI
                     VGLRAMISSIFGVDAQEAMMLLQAMIGLKMINVDLSYPILETGLIKIISNDHYIGLNA
                     NGYY"
BASE COUNT         9280 a         8882 c         9226 g         8365 t
ORIGIN      
        1 atgttgaaaa taaaccttcg atttgtttgt caagatattg tccatcttta tataatttgg
       61 gggtagggtg ttctttatgt aaaaaatagt gtcacctaaa tttgcgtcag tgaaatttat
      121 attaagtaga tcttcgaaat aaaaccttga caaggttaga acgaacccgg acaggttctg
      181 tcctgtcata ttgaaatcaa taaagttggt tccggcaaag actttcatcc tgtttttatt
      241 ttcactgtgc tcaatatata acttcgtcat tttttgatac aggctttgaa tatcttttat
      301 attagtcggt aagtcagctc gttgttcagc aataaaatta gtatctgaaa acatggtttg
      361 gacactgaat cttactggta ttccctcttc tacgctaatt ttagttatga ttgccttgtc
      421 gggtttagga ttagcgacaa caatgattcg acattcagca cttttgttct tttcgtatct
      481 aatgttatta atttgattaa gtatttcctg tgcctgccgc gtaagtggtg tcgaacttat
      541 cgattcaatg ttagtaataa gacttacaag ttgagccaca acttttgagt agtctgcatc
      601 tgtatatttg gtgttcgtgc ggcacgcata aatttgtggg aggtgttgcc gcgctaaatc
      661 atattgttca gggttgtatc cttctccaga gaatgtggaa ttaatgggtt gttgttcttc
      721 ccgattatca caaggccaca tcggtgtgtc tggtgtttta acattgtcgg tatctgttgt
      781 gcggttataa atatcgccgt gatatgtatt tatcatacct ggggtaaagt caattgacat
      841 aacattcctt attcattatc tgttttatgc taagattttt aaattggata attagagcag
      901 gaatggattt tcttctacgc acaataaata tcatgcgtca tatcttcctg atataattac
      961 ctttgacttc ccttttatct ccacccggta atatttgtgt ataaaaaagg gcattgatag
     1021 tgtcgtaaaa gaaaacgtta acaactatca aaaagcgctc attttaaaaa gttatgatgt
     1081 tctccgatag tgtgtgaaat gatatttttg tgatgtttta gatgtgtaat aataaaatgg
     1141 gttaaggtat cttataatta tttaataatt tgttgtgtaa agcactgata aatcataatc
     1201 cagtacgtct ttaaactgct gcactgtatc gctgatcact aagcagatca ctgatacacc
     1261 atgctggacg cgataattgg aatctcacgc agggagtaat gtgtaaatag caaaatcgcc
     1321 tgatgcgcta cgcctgtcag gtctacaaat aacatacaat ttattgaatt ttcatgactt
     1381 tgtagatcgg ataagacgcg ttagcgtcgc atccgacata aacaaagcgc actttaccaa
     1441 ctatcggcag tggatatcgc cgctttattt caaccgctta cccgcttcat caaccacttt
     1501 ctcgccatct tccttggaga acgcgccttt ttgcgcatct ggcagaattt ccagcaccac
     1561 ttccgagggg cggcataggc gagttcccag cggcgtcacc acaatcggac ggttaatcag
     1621 aatcgggtgc tgaagcataa agtcgattaa ccgctcgtca gtaaatttat cttccgcaag
     1681 gcccagttcc tcatacggct cgacgttttt acgcagcagc gcgcgtacgg taatccccat
     1741 atcggcaatg agttttacca gttcatcgcg cgttggtggt gtttccagat aatggataat
     1801 ggtcggttct gtaccgctgt tgcggatcat ctccagcgta ttacgcgatg tgccgcaggc
     1861 cgggttgtga taaatggtaa tgttgctcat atcagtatct cattacaaag tgaaagagag
     1921 acgaagcgcc agcgcgacga gtgtcacaaa cagcacaggc agggtcatga taatccctgt
     1981 acggaagtaa tatccccagc tgatagtcat attcttctgc gaaagtacgt gcagccagag
     2041 cagcgttgcc aggctaccaa tcggggtaat tttcggtccc aaatcgcagc caatcacatt
     2101 ggcataaacc atcgcttctt tgataacgcc agatgccgtg ctgccatcaa tggacaacgc
     2161 gccaaccagt accgtcggca tattgttcat aatagaagag aggaaggcgg tgaggaatcc
     2221 ggtgccgagc gtcgcggccc acaggccgtt atccgccagc acgttgagta cgccagaaag
     2281 atattccgtt aatccggcat tgcgcaggcc ataaaccacc agatacatgc cgagcgagaa
     2341 gatgacaatc tgccaggggg caccgcgcag gactttaccc gtattaatcg catgaccgcg
     2401 tttagcgacg acaaataata tcagcgcgcc cacagctgca atggcgctca ccggaatgcc
     2461 gagcggttcc aggacgaaaa atcccaccag cagaagcagt aaaacaaccc agccagtttt
     2521 gaacgtagca ggatctttga tcgcttctgc gggagatttc agcagcgcca tatcgtagtt
     2581 ctgcggaata tctttgcgaa aatagagatg taacatcacc agcgtggcaa caatcgcggc
     2641 gatatccacc ggcaccatca ccgaggcgta ttcgcgaaag ccgaggccaa agaaatcagc
     2701 ggaaacgata ttcaccaggt tggagacaat aagcggcagg ctggcggtat cggcaatgaa
     2761 tccggccgcc atcacgaacg ccagcgtagt gcctttactg aaccctaaag ccagcagcat
     2821 ggcgatgaca atcggtgtca aaataagcgc cgcgccatca ttggcaaaca gggcggcaac
     2881 ggcagcaccg agcaggacaa tccaggtaaa cagcaagcga ccacgaccat taccccagcg
     2941 tgagacgtgc agcgccgccc attcaaaaaa gccggactca tccagcagca ggctgatgat
     3001 aatgacggcg ataaacgcag ccgtcgcgtt ccagacgata ttccacacca ccggaatatc
     3061 acccggatgg accacgcccg taactaacgc cagtactgcg ccgagcgttg cactccagcc
     3121 gatgcctaaa cctttcggct gccagataac caatacgatg gtcaggacaa agatagcgcc
     3181 tgccagtaac ataatgcctc ccggataaaa cacatctgaa aattcatatg tgtttagcta
     3241 aatttttaac tgcaaatgtt cttactgtcc ccggaacagt tttgtcgagc caggttgcgg
     3301 acaatcgcct gaaccttttc ctgttcacat cgccaggcct catcaataat tttcgccgcc
     3361 catgctggaa tatgcggtga taagcggtaa tgaacccact taccttgctt gcggtccagc
     3421 aatagcccgc tttcacgcag caatgccagg tggcgggaga tcttgggctg cgactggtcg
     3481 agagcagtgc agagatcgca gacgcataac tctcccagtt cgctgagcag taaaacgatg
     3541 cccagacggg tttcatcagc aagaattttg aacaattgga tgggtaacag aaatgacata
     3601 ttgcgctcct gattgttgca ggtagtgtct ctcttcgaag cggataagtc aaaaacatat
     3661 atgacttaac gaatgtgtaa gtgcagagga aggtaatagg tgtgaatttt gagttggcta
     3721 ttcatttgaa aggaggtctg aatcaggacg taaaaaaacc gactttgcgt cggtttttta
     3781 ctttccagcc ctgagttggt ggctctggct ggagtgagag agttcttatc taacagctca
     3841 atactattag ccagtggcta acattgcaag aattagcgat ttcttcagct ggcggagggg
     3901 gcacttcggt atatatcgca atgacgattt atatactttc actgggtcat cgtcatatta
     3961 agccttgtcc gaatccggat gtaaaaaaac cgactttgcg tcggtttttt actttccagc
     4021 cctgagttgg tggcactggc tggagtgaga atgccattat ctaacgcatc aatgctaata
     4081 ccatactgaa actattgcaa ggacatgctg gttttataac ctgaatgtac tgtatgatta
     4141 tccagttagc tctgaggcat tttcactctg gtaatgcgca taaacgcttt caaagtcctg
     4201 gtcagaagta cgggtggtgc cgttaactga tgctctggcc ggagtgagaa tgccattatc
     4261 taacaatagg gcatgcgccg tgacaggcag tggatgagta agcggatgca ttctcactcc
     4321 atcgcatgga gaaaacgggt gattgataaa gcaatcatcg ttcttggggc gttaattgcg
     4381 ctgctggaac tgatccgctt tctgcttcag cttctgaact gatagcggaa acgtaattaa
     4441 gggctaagag cacactactc ttagcccttt aacatttaac gcattgtcac gaactcttct
     4501 gccgccgttg ggtgaatggc gacggtattg tcgaagtctt ttttggttgc acccatcttc
     4561 agcgccaccg cgaagccctg caacatttcg tccataccaa agccaatgcc gtgaataccg
     4621 acaatcttct cttccgatcc aacgcacacc agcttcatgc ggcacggctg gcggtgagtg
     4681 gtgacggcgg tatacatcgc ggtgaaagag gatttataca ctttcacctg atcgtcgcca
     4741 tactgctcgc gcgcctgcgg ttccgttaaa ccaacagtac caatcggcgg atggctgaag
     4801 accacggtcg gaatgttgct gtaatccaga tgctcatccg gcttgttatt aaacaggcgt
     4861 tcagagagac ggcgacccgc tgcaactgcc accggtgtca gctccactgc acccgtgtta
     4921 tcgcccaccg cgtaaatacc ttcaatattg gtgttttgat atttatcgac gacgatatag
     4981 cctttttcgt tagttttaac gccagcggct tccaggttga tgttgtcatt ggcaggctcg
     5041 cgaccaatcg cccaaatcag gcaatccacc gtttcactgc gaccatcttc cagctccagc
     5101 gtcaggctac catcggcatt tttcactacc gctttcggga tggcgttggt gtgcagctgc
     5161 gggccttcgg cgttcatcac ttcgaccagc gtttcggaaa tcatcgggtc gaagctgcgc
     5221 agcggcgcat gtttacgcac aaacagatgc gttttcgcgc cgaggccgtt aatcacgccc
     5281 gccagctcaa cggcgatgta acccgcgcca acaaccgcca cgcgctctgg caaagcagga
     5341 agggcgaaga agccatcaga atcaataccg tattccacgc ccggaatatc cgggtggctc
     5401 ggacgaccgc ctgtggcgat cagaatatga tcggccgtga tggtttcgcc gtttacctcc
     5461 agcgttttgg catcaacgaa gcgggcaaag cctttgatta catcaacgtt atttttaccg
     5521 agcacgtttt cataggaagt atgaatacgg tcgatatagg cggtacggct ggcgatcaac
     5581 gtttcccagt tgaatttatt gatagtggta tcaaaaccat aatccgggcc gtacatatgg
     5641 atcgcttcac ggatttgcgc cgcgtgccac atcacttttt tcggcacaca gccaacattt
     5701 acgcaggtgc cgcccagctc tttggcttca atcagcgcac atttctggcc gtacatagcc
     5761 gcgcggttga tggaggcgat accgccgctg ccgccgccga tggcgatgta atcatagtgt
     5821 ttagtcatga caaagtgtcc ttatcgttga ttaccgcgat tgtagcgcga gacgtaatag
     5881 gtgccagcaa tggctgcaat tactccggca cgatccagct tacggtggcg tgcccggtgc
     5941 ctgccggaac cagtttgctg tgcagccacg gcagcacgtt attcatctgc tgttccagtt
     6001 tccacggcgg gttaatcaca atcatgccgg aagcggtcat gccacggcga tcgctgtctg
     6061 gcagtaccgc cagttcaatt tgcagaattt tgcgaatacc ggtcgcttcc agatcgtgga
     6121 tcatgcgctt aatttgctga cgcagcacca ccggatacca cagtgcgtaa gtaccagtgg
     6181 cgaaacgttt gtaaccttct gctatcccgc tgaccaccgc ctgatagtcg gttttcattt
     6241 catacggcgg gtcgataagg attaaaccac ggcgggaaac cggcggcagt ttggccttaa
     6301 gctgctggaa accatcggct ttttcgacgc gcgcacggct gtctttctga aattcagaac
     6361 gcagcaacgg gtaatcgctc gggtgcagtt cggtcagttg caggctgtct tgttcgcgca
     6421 gtagctggcg agcaatcaat ggcgaaccgg ggtagtaacg caactgaccg ctacggttga
     6481 agtgttttac cacattgatg tacgcctcca gttctgcggg caaatcgtcc tgctgccaga
     6541 tacgggcgat gccttcgaga tattcgccgg tacgctcggc atgttcgctg cctaactgat
     6601 aacgcccggc ccctgcgtgg gtgtcgagat agagaaacgg tttatctttc tctttcagcg
     6661 actcgatgat caggctctga acggtatgtt taaggacgtc ggcgtggttg ccagcgtgaa
     6721 agctgtggcg ataactgagc atgggtaaag gtgttccggt aagtaaaaat cggcccgtat
     6781 tcggggcgca caaaagctta tcaggacagt ataccgaaaa gaggccgccg ccgcgaaagc
     6841 gtaacgtttc tcattgaaat tcactacact taaccccatg ctacacacat tatgtaaagc
     6901 gcctgttgag cgcttcctta acctctttaa ccaggactgc gcgaatgacg aatccgttac
     6961 tgactccctt tgaattgcct ccgttttcta aaattctccc ggaacatgtc gttccagccg
     7021 tgactaaggc gctgaacgac tgccgcgaaa atgtggagcg cgtagtagcg caaggagcac
     7081 cgtacacctg ggaaaatctc tgccagccgt tggcggaagt ggacgatgtg ctggggcgta
     7141 tcttctcccc ggtcagccac ctgaactcgg tgaaaaatag cccggaactg cgtgaagcgt
     7201 acgaacaaac cctgccgctg ctgtcggaat acagcacctg ggtagggcaa catgaagggc
     7261 tgtataaagc gtatcgcgac ctgcgcgatg gcgatcatta cgccacgctg aacacggctc
     7321 agaaaaaagc ggttgataac gcactgcgcg acttcgaact ctctggcatc ggtctgccaa
     7381 tagagaaaca acaacgctac ggtgagattg ccactcgtct ttccgagctg ggcaaccagt
     7441 acagcaacaa cgtcctcgat gcgacgatgg gctggaccaa actcgttacc gacgaagcgg
     7501 agctggcggg gatgcctgaa agcgcgctgg ctgcggcaaa agcccaggcc gaagcgaaag
     7561 agctggaagg ctacctgctg acgctggata tcccaagcta cctgccggta atgacctact
     7621 gcgacaacca ggccttgcgt gaagagatgt atcgcgctta cagcacccgc gcttccgatc
     7681 aaggcccgaa cgccggtaag tgggataaca gcaaggtgat ggaagagatc ctcgctctgc
     7741 gtcacgaact ggcgcaactg ctgggctttg aaaactacgc ctttaaatcc cttgctacta
     7801 aaatggcaga aaacccgcag caggtgctgg atttcttaac cgatctggca aaacgcgcgc
     7861 gtccgcaagg cgaaaaagag ctggcacaac tgcgcgcctt cgccaaagcg gaatttggcg
     7921 tcgatgagtt gcagccgtgg gatatcgcgt actacagcga aaaacaaaaa cagcacctct
     7981 acagcatcag tgacgaacag ctgcgtccgt acttcccgga aaacaaagcg gttaacggcc
     8041 tgtttgaagt ggtgaaacgt atttacggca tcaccgctaa agagcgtaaa gatgttgatg
     8101 tctggcatcc ggatgtacgt ttcttcgaac tgtatgacga aaataacgaa ctgcgcggca
     8161 gcttctacct cgatctgtat gcccgtgaaa acaagcgcgg cggggcgtgg atggatgact
     8221 gcgtaggcca gatgcgtaaa gctgatggtt cgctgcaaaa accggtcgcg tatttgactt
     8281 gtaacttcaa ccgcccggta aatggtaaac cggcgctgtt cactcacgac gaagtgatca
     8341 ccctgttcca cgagttcggt cacggcctgc accatatgct gacccgcatc gaaaccgctg
     8401 gtgtttccgg tatcagcggt gtgccgtggg atgcggtcga actgccgagt cagtttatgg
     8461 aaaactggtg ctgggagccg gaggcgctgg cgtttatctc cggtcactat gaaaccggcg
     8521 aaccgctgcc gaaagagttg ctggataaaa tgctggcggc gaagaactac caggcggcgc
     8581 tgtttattct gcgtcagctg gagttcggcc tgtttgattt ccgccttcat gctgagttcc
     8641 gcccggatca gggggcaaaa atcctcgaaa ctctggcaga aatcaagaaa ctggttgccg
     8701 tggtgccatc tccgtcctgg ggccgtttcc cgcacgcttt cagccatatt ttcgccggtg
     8761 gttatgcggc aggttactac agctacctgt gggctgacgt actggcggca gatgctttct
     8821 cgcgctttga ggaagagggc attttcaacc gtgaaaccgg gcagtcgttc ctcgacaaca
     8881 ttctgagccg tggcggttca gaagagccga tggatctgtt caaacgcttc cgtggtcgtg
     8941 aaccgcagct ggatgcgatg ctggagcatt acggcattaa gggctgatca ttcagtgaaa
     9001 atctgcttaa ttgatgaaac aggcgccgga gacggcgcct tatctgttct ggcggcccgc
     9061 tgggggctgg agcacgatga agacaacctg atggcgctgg tgttaacgcc ggaacatctg
     9121 gaattgcgca agcgtgatga gccaaaactt ggcggcatat ttgttgattt tgttggcgga
     9181 gcgatggcgc accgacgcaa attcggcggt ggtcgcggtg aggcggtggc gaaagcggtg
     9241 ggcattaaag gcgattattt gccggatgtg gtggacgcca ccgcagggct ggggcgcgat
     9301 gcctttgtgc tggcttcagt cggttgccgc gtgcggatgc tggagcgtaa tccagtcgtt
     9361 gccgcattgc tcgacgacgg cctggcgcgt ggttatgcgg atgcggaaat cggcggctgg
     9421 ttgcaggagc gtttgcagtt aattcacgcc tccagcctga cggcgctaac cgatattacc
     9481 ccacgcccgc aggtggtcta tctcgacccg atgttcccgc ataagcagaa aagcgcgctg
     9541 gtgaaaaaag agatgcgtgt ctttcagtcg ctggtggggc cggatcttga tgccgatgga
     9601 ttgctggagc ctgcacgctt gctggcgacc aaacgcgtgg tagtaaagcg cccggactac
     9661 gcgccaccgc tagcgaatgt cgccacgcca aacgcggttg tgactaaagg gcatcgcttt
     9721 gatatctatg caggtacgcc cgtgtaaaag taaagcaggg tagcgataac gctaccctgc
     9781 tttctgcaag aataattaat ggctttccgg cgtcgcaatc atgcgtttca gccacggcac
     9841 catcaacagc atcactactg caacgcccag tgtgaccaga ccaatcttac caaacacgtt
     9901 ggtatagacg ggcaacgtct caagcggatc ggtaatgttg tccggcaccg cggtaaatgt
     9961 tgccacatag ccgcccagca agaacgcggc agcctgcgtc aggaaccaca tcccgagaat
    10021 aaagcccatc aaatgctgcg gcaccagggc agcaatcatc gccaggccaa gggcgctaat
    10081 aaacagttca cctaagctct ggaataagta caccagcacg ataaaccatg gcgatgtcag
    10141 cccctgggca tccgcaaacc acattcccgc agctgcagcc gtcaaaaagc ccagagagca
    10201 cataaacatg ccgagagtaa atttcatcgg catcgagagg tctttgcctt tgttacccag
    10261 atgcgtgtaa atgcctgcca gtattgggct ggcgagtacc acccagaacg ggttaagcgc
    10321 ctggaagctg accgggttga tggaaaaacc gagaatttca tgatgcacgt tgttgatggc
    10381 aaagaagttc agcgatgttg gcatctgggc gtagagaatg taaaacacca ccgcttcgag
    10441 catcaggaca aaggcgacaa acattttatt gcgcccggtt ttatccagct tgaatgcctg
    10501 acgaaagaag atgatggtga cgacgatgga gagaacaatc agcaccagat tggcgacttc
    10561 tacgttgtgc atcagccatg cacatacgaa gatcatcacc acgctgccaa gtaatacgta
    10621 caacagtttg ctgaagctca tcggcttgaa gtcgggttca gaaccaatgt ctttcaccat
    10681 tccacgacag gcgatgtaaa ccagtaatgc gataattaac cccgccccgc acaggttgta
    10741 ggtgactgaa taaccgaatc tatcagcgat cacaggggcc agcgataacg ctatcaacga
    10801 gccgatattg atcgacatat agaacagggt gaatgcgcca tcaagccgcg gatctttcgg
    10861 cggatagcac ttcgaaagca agctggctgg gttagcttta aacaggccgt taccgacagc
    10921 gatagtcccc agggcgatga aaatcaggtc aggcttaagt agcgacatgc cggtcatgaa
    10981 gtagccaatc gccagcacaa gtgctccgag aacaatggtg cgtttggtcc ccagcaggtg
    11041 gtcgccgaca tagccgccaa tggaaatgag gccatagacc agcgcagcaa aagcaccaaa
    11101 agtgacaaaa gcctgctctt gcgagaatcc aagctgttta acgaagaaaa ccgccagtac
    11161 gccctgcacg ccgtagtagc cgaatcgctc ccataactcg acaaaaaaga tcatgaaaaa
    11221 tgggcgaggt tgctgcagca tccccatggg tgttgttgta ttcatattta ttaaccttcc
    11281 aataccatcc tgaaaagtat gaacgcgtaa gggataacgc gcgaaaaagt ttacatctgt
    11341 cagagattct taatattgat gcggtgaata gattaataca ctattacaac agaaaataac
    11401 cagctgatca cactaatgat ggaaaatatg atggtgttaa atgttgtatt ggcaggagat
    11461 tttgattgcg tacgcagatc gtaggccaga taaggggttt acgctgcatc aggtaacaag
    11521 gcgcgccaaa aagcaaaacg cccgcatatg cgggcgttca ggaacacgtc gtggggggat
    11581 gattattctt cttcgtcgcg cagcggaaca atcagcatat caacgtgaac ggtgttgatc
    11641 agctgacgtg cggaagacat tagtttgctc cagaagtcct ggtggtgacc acaaaccacc
    11701 agatccatat cgtatttctt gattgcatcg accagaacct ggcccaggtc gccgctgccg
    11761 ctcagggttt cagtgattgg gtagcctgca ttagtggaaa gctcggtcag cgcatgatgt
    11821 gtctcttcag agatgcgttt ctgcatatca cccagattca catcaataag cccggtgtat
    11881 aggtcagagt agtttacatc tacgtggatc agagaaactt tcgcattgta ggggcgagcc
    11941 atagagactg ctttctctac cagaactttg ctttccgggg agaggtcgac cgcgatgaga
    12001 atgtgtttat aagccatagt gttactcctt ccataaagtt gtcgatgact ggccagctag
    12061 cgtttcttgt gccgttacgt cacccgcgtc ctgcgaacta cgtgcgcggg gctaacccag
    12121 ccgtaaagat tcagtgttaa gaccatcctt accttatagc gacccggatg atccgtcaat
    12181 ccgccttgct tactcgttag ataaacaaat tttcgacatg aatatattga tagtggttaa
    12241 ccttctggaa aaaaaacaac ctgatctcct acactatcta tagagccgct cggatgttgg
    12301 tcaccacaaa agcggtttct ccaggacctg gtgttaactg attggcagcg tatcgggagc
    12361 ggtagccccg gggtggaact ccgcgggcag gtcgccgggg aggaggtatg ataagcaccg
    12421 tcgcattatt ttgggcttta tgtgtcgttt gcattgttaa catggcgcgc tatttctcat
    12481 cactacgcgc gttgttagtg gtactgcgta actgcgatcc attgctctat caatatgttg
    12541 atggaggggg cttttttacc tcacatggcc aacccaacaa acaggtgcgt ctcgtttggt
    12601 atatctatgc ccaacgttat cgcgatcatc acgatgatga gtttattcgc cgctgtgagc
    12661 gggtgcgtcg gcagtttatt ctgactagcg cattgtgtgg tctggtggtg gtcagcctga
    12721 ttgcattgat gatttggcat taagcacttc agcaataata acgcataaaa aaagcgggcc
    12781 agtaaactga cccgctcgtt ttggtgcgta cgattacagg aactgcaagg agagccagta
    12841 cagcccgccg gaaagcagta ccgcagccgg aagggtaaac acccaggcca tcagaatact
    12901 ggttacggtt ttacgctgta agccgccgcc atctaccacc atcgtccccg cgacagaaga
    12961 ggagagtacg tgagtagtgg aaaccggcat cccggtataa ctcgccaggc cgatagacac
    13021 tgccgccgtc atctgggcag acatcccctg agcgtaggtc atgcctttct taccgatttt
    13081 ctcaccgata gtcgttgcca cacggcgcca gccaatcatc gtaccgatac ctaacgccag
    13141 cgcgaccgcc atgatgatcc acaccggtgc atactcgatg gtgctaagca tgtcggactt
    13201 cagtttcttc aacaggcgct gatcgtcagc actcacgcca ggcatcttca ccactttgtc
    13261 gatagtgtca gaaacgcaca gcataatgcg gcgcatctgg ctacgttgat caagcgacag
    13321 cttgtcgtag ctttccacat cggtggtcaa catacctttc aggcggttga gcgcgttaat
    13381 ggtattcgac ggatggcagt ggaactccgc aggttgcgtt gcgccagctt ccggagccgg
    13441 tactaactga tcagcaccgg tagcctgttt gagcagcgca ggatgctgct caaagtaagc
    13501 ttcgacgttg ttgatggcat cacgggtacg ggtgatttcg tagccagtgg cattcatgtt
    13561 caccacgaag cctgctggcg cgacgccaat caataccaac ataaccagac caatgccttt
    13621 ctgaccatcg ttcgcgccgt gcgaaaacgc cacgccgata gcggaaagga tcagcgcgat
    13681 acgcgtccag aacggcggct ttttcttgcc gtctttcttt tcacgctccg ctggggtcag
    13741 gtggatacgg gcgcgtttct tggtgccgct ccagtagcga cgcagcaaga aaatcagacc
    13801 gccagcaaac accaggccga caatagggga aacgatcaga gaaccgaaaa tacttaatac
    13861 tttcgggata ttgagtgcat ccaccactga cgtcccggtc atcaacgcat tggttaaacc
    13921 aatcccgatg atcgcgccaa tcagcgtatg agagctggat gcaggtaaac caaagtacca
    13981 ggtacccagg ttccagataa tcgccgccag caacatagag aacaccatgg caaggccatg
    14041 agacgatccc atattaagca gcagatccgt cggcagcata tgcacaatgg cataggcaac
    14101 actcagacca cccagcaaaa cacccaaaaa gttgaacacc gccgccataa ccacggcgag
    14161 ctgagaacgc atcgcgcggg tatagataac ggttgccacg gcgttggctg tgtcatggaa
    14221 accattgatg gcttcgtaga acagcacaaa agccagtgca agcaataata acagcccggt
    14281 atgcaaatcc aggccagcaa acaaatgtag cataggacgt tacgccattt tgaggacatg
    14341 aacgcggcgc attatcagtg actttcgcgg cgcgggcaaa gtgaaatata gacttttttt
    14401 gatttgcctc ctgtatggat ttcactcaaa aaataattat cttatataat tcaggcaaat
    14461 acttcctttt agtaatattg atgctggtgc gaccactgag gaatctttac aattcacgcc
    14521 cgttttttct aagaggagcg caacgtggaa aggtttgatg ccattattat aggcgctggt
    14581 gcggcgggta tgttctgttc tgcgctggca ggtcaggcag gacgccgggt tctgctgatc
    14641 gataatggta aaaaaccagg gcgcaaaatc cttatgtctg gcggtgggcg ctgcaacttt
    14701 accaaccttt atgtcgaacc aggcgcttat ctgagccaga atccgcattt ttgtaagtct
    14761 gcactcgcgc gttttaccca gtgggatttc attgatctgg tcaataaaca cggcatcgcc
    14821 tggcacgaga aaacgttagg acaactcttc tgcgatgact ccgcgcagca gattgtcgac
    14881 atgctggtgg atgagtgcga gaagggcaat gtaaccttca gattgcgtag cgaagtgctg
    14941 agtgtggcga aggatgaaac aggcttcacg cttgaactga acggcatgac tgtcggttgc
    15001 gaaaagctgg tcatcgcgac cggtgggctg tcaatgccgg ggctgggcgc gtcgccgttt
    15061 ggttataaga ttgccgaaca atttggcctc aacgtgctgc cgacccgcgc gggtctggtg
    15121 ccattcactc tgcataaacc gttgctcgaa gagttacagg tgctggcggg cgtggcggtg
    15181 ccttccgtga ttaccgctga aaacggcatc gttttccgtg agaacttact cttcacccat
    15241 cgcggcttgt ctggaccggc ggtgttgcag atttccagct actggcaacc gggtgaattt
    15301 gtcagtatca atctgctacc ggatgtggat ctcgaaacct tcctgaatga gcagcgtaac
    15361 gcacatccga atcagagcct gaaaaacaca ctggcggttc atctaccgaa gcggttggtt
    15421 gaacgcttac agcaactcgg gcaaatcccg gatgtttcgc taaaacagct caacgtgcgt
    15481 gaccaacagg cactgattag cacattgacc gactggcgcg tacaacccaa cggcactgaa
    15541 ggctatcgca ctgccgaagt gacgctcggc ggcgtggaca ccaacgaact ctcttcacgg
    15601 acgatggaag cgcgcaaagt gcctgggctg tacttcatcg gcgaagtgat ggacgttacc
    15661 ggctggctgg ggggctataa cttccagtgg gcgtggtcga gtgcgtgggc ttgtgcgcag
    15721 gatttgattg ctgtgcagcg acaataaagt ttgtacattg ctcataaagt ttgtacataa
    15781 ggcgattctg taacaataaa gtttgtacac tgatctatac tgcattctgt tttctgtaat
    15841 ggagtgaagt tccttgggcc gacgtagaaa gttagaggca ctggctgatt ttcagcgagc
    15901 attaaagaat aaatacggtc ttggcgaagg cgacagctac aagccatggc ttcgggtaca
    15961 agatgttcgc tctcggggta atagcgggaa gattcagggc atcaaggtca accgagagca
    16021 tcacatgctt tctgaacacg aaagttgctt tttctacatt gccgagtttt gcgattccgt
    16081 catagatatc agggagcagt tccctctctt acctctagac ctatcggtaa aaattgccaa
    16141 aacctttggg gtggatcatc ccattgtacc tggatccaag tcgcctaatg tcatgaccac
    16201 ggactttgtc ctgactcgct ctgacggggt gaacacctgg tatgaagccg tgagtgtcaa
    16261 acctgcagag aagctgaccg ataaaaggat tgcggagaaa ctggatatcg agcgtaactg
    16321 gtggcagttg cagggagttc cgttccatct gttcgtgatg accaaacaaa accaaatcca
    16381 gtcaaaaaac attcaatggg taacggctcc tgtccggcaa ggccgaaaat ttccaactga
    16441 cttagtcgaa agcgctttag cactgatttt tcccggaacc atgttgacag aggagatatg
    16501 cgatgagttt gttcgagaat ttaaaattga gcatgatgat gctttggtcc tgttaaaaac
    16561 cttgattgcg accaagatgg ttgatgttga tctcgataag cccatagctg atacaggtat
    16621 tttgaatatc gtcaaagtta cagactttaa agtggtacgg catcatgcaa gttgagcgta
    16681 acagtgtttg gcaggtcact agaattgacg gcattaagga cggtatgtac agagtccttg
    16741 agcactatgc aaatgatgac ctcttgattc tttttcggct gcatgatgga aaagctctgg
    16801 aaaagccagt tccagtagag cttcccctat ttttagagtc tgccaagtgt ggaaatagca
    16861 aaatatccag ttatccgata cctttttacc aactaatatc tgaagaagag atctcccctg
    16921 ctcacaggga taagcggaat cagcgcttcg aattgataca tgatttgatc agtaatccgg
    16981 aatttttgct tgatgtagcg ttaaatcagc gtagcaaaac cgtagctagt catgccaagg
    17041 aacgaaaggt ctatgtccaa aaaatctatc gctcgctaaa tcaatactgg cgatacggtc
    17101 aagaaatcaa tgctctactg ccagcataca aacattgtgg tggctcaggt aaaccccggt
    17161 tgtccggtaa ggcgaaaaga ggggcaccga tccaactgtc gacacccagt atggtggccc
    17221 cttcgggagt gaacacaacg gaagaagata aggccaaatt tcttctaggc atgaaaaagt
    17281 atggactaaa gggaaagaag gtttctctaa gtcgtgtata tgaacaaatg cttaaagagt
    17341 actatgctga tgaactccga tcagccgaaa aagaaaatcg agagcctgag atccctagcc
    17401 ttaggtcatt tcgttactgg attaaaaagc tgattcctga agccgagttg atccgtaagc
    17461 aaacgacggc tggggacttc gaacgaaaca ggagaggtct gcgaggagcg gccacggatc
    17521 atacggaagt gcctggtagc tgttttgagc tggacgcgac agttctggat gtccatatag
    17581 tttcagagtt caaccgaaac catgtgcttg ggcgtcctac agtttactgt gtggtggata
    17641 aagagagccg aatgatggtc ggccttcatg tatccatgga gtatgcttcc tggcgagcag
    17701 gaaggcaggc actggtgaat agctttacct cgaagaaagc ttattgtgcc cgttttggca
    17761 tcgaaattga agaatcggaa tggccctgtc atcacattcc ccagcgcctc ctgtgtgacc
    17821 gaggggaatt catctgtaat aagccggaag agcttgcggt cccattgata gggcatctga
    17881 gcattgctcc tccctaccgt gcagagttga aaggtatcgt cgagcaccga ttccaaattc
    17941 tgaacgaaaa gcttgtccat gagctgatgg ggacgacgaa ggggcgccat tatatccgtg
    18001 gagaccgcga tcctaggttg gacgccactt tgactctttc tgaggtaacc aagcttctca
    18061 ttgatcaggt tttagagcac aacagctcta tttttgatgg actggctggt caaacacctc
    18121 tgctagtgga gtcagggctt ccccctacgc cactaaacta ctggaataca catctgaaaa
    18181 gacaccgcca tgcattaaat aaagcggatg aagcagatat cagagcccgg ctgcttcccg
    18241 ttgagcaggt ttctatgacc agcaaaggaa tccgcctcaa tgatgatatg tattacgaat
    18301 gtgaccgagc agagtttgaa gactggaagg tgatagctcg ctcgaatgga cgttggaagc
    18361 tggaagccag gattgaccaa gataacgcct cctttatcta tgtgcgcctt cgtccaagcg
    18421 aaggttttac tcggtgtacg ctgatgacgc gctcgtctag ctttgaggag cgccatagag
    18481 cagatgtact gtactttgag gattggaaaa aggtttcgaa aaaacgttct aaaccgacca
    18541 gtaaatccat tgaaaggcac aatcgaagaa aaaccatcac ggcgaatgct agggaagaac
    18601 ttaaaaaaga acctgctctc aaaacaaagg cagaaaaaac cctcggcata aaagagcggc
    18661 gacgagaagc cataatggaa gccaggattt ccagtgatga tagtaactat acggagcctt
    18721 cagaaaatca gtcatcagtt gataaattag ctgctgatag agccagcaag gtcgtgtcgt
    18781 tgctgaagag ggagcggggt tctaatagtg aaacttgaga aagcaaaata ccggcctgcg
    18841 attctccccg agcatcgagg taacccatta attgaggcgc taccagcgaa gcatactgat
    18901 gaggagcttg tagacaagct cagtttttac ccaccgcact catttgagga aactcaactc
    18961 gcccccttcg aacgagttga gtatctgacc agactgaaag atctacggca accattaccc
    19021 atctatctgg actgttttag agcggtggaa atggcaataa aggaggggta ctcggtcaag
    19081 aatccgcttt caccgacgac gatgaattac ctacactatc caacagatcg tcgtccaaat
    19141 attgaaccaa gaactggata tttcaagcca aaaggatgcg gaatcacggt aataggtgaa
    19201 agcggtgttg gaaaaacttg catgctcgaa cagatattag gatgtttccc cgacacaatt
    19261 gagcactcgc aatataaaaa tcattcgata ccattacgcc aagtagtatg gatcaaagta
    19321 gattgcccgg atgactccag cgttagagct ctgtgccaca agattctgtc cgaattggat
    19381 agcaagcttg gctttgagcc gactaagcga gccggaacta tccctcttct gctagaccaa
    19441 atcgaagctc gtatcaaatc cagcttttta gggatcttgg tcatcgatga gatgcagaat
    19501 ctgaatctgg caaaaaccgg tggtgctgac cgcctgctgg gtttcatcca caatctggtt
    19561 aacaatctcg gtattcctat cctgttctgt gccaacccgc cgttcaatca gttgctcagt
    19621 agaacgctta aagcggcacg acgggcagaa agcaatggtt acttcgatgt tgtgctgatg
    19681 gaaaacgacg gtgtatggga actgtttata gaggagttgt gggagcttca atggaccaac
    19741 gtaaggactc cattgactgt agagttgaac gacaagcttt atgagctatc tgttggcaac
    19801 atggatctgg ccgttcgaat cttccgtgaa gcacagcgtt atatcatagg ttgtggcgat
    19861 gaacgcatca caggagagtt gcttgaacat gccgcaagta ttgctatcaa ggcatccaag
    19921 gcggtcaccg atgaaatcag gcgagaacgc tcaattactg cgttgaaaag aaaacggaac
    19981 attacttcac ttccggccag cgcagatatg gctcgtacac agaagacgga attggtggca
    20041 attgagggag tgcctcaatc aatacccggt gacctgagtc gacctcacca tcctgagttt
    20101 gccgatgcgc ttaccatatt gcaaactgcg gatgaccttt ttgagcgaat cggcaatccc
    20161 gatctaatgc agcatgcggc ggctgaggag aagccactgg agctgttgcg gcaagaaggc
    20221 gttttctgtg aacatccgct gaaggtcttc gcgtaaaggt cttaagggcg taccgcatga
    20281 aattgcccgt aagcctaccc gatgaattac tactaagtcg cctgatccgc tatgttacgg
    20341 tttcaggcga taaaggggat aactttgcta gcaaggtttt tgggtcaaga aaggtatcca
    20401 tccatccctt tctgacagcg aggcttgagc agctggccgg atggggcctt gagagtgctg
    20461 aagtaatgtt gtttcagcaa acgctggcac cgttgtttct tttttttctc ccgacttatg
    20521 ctgataggct taagcgatca atgctggcag gtgatggcgc agaggcacat cgagcaagcc
    20581 agctgtcttt atttggttgc ggtaattcgc tttgcttaaa atggtgcccg gtgtgtgctc
    20641 aacaagattt gcgcctgtac ggtgttgcct attggcaccg gacacaccag atacctggcg
    20701 taacggcttg tgcatttcat cccgtactcc tggagaagct ggaactggtt aggcgacagc
    20761 ggatcattgc ggaattactc cccactctca tcgaccagcc acgagttgct tttgatgctg
    20821 aggttcaggt agccaggttt ggcttcaagc ttcttcaatt ggtcacgggc ggtatccccc
    20881 gtgttgacac tgtattaacc taccgctcga agctgtctga gttggggttt attacagctc
    20941 atcatcgagt aaggcgtaaa agtttaatgg aggacttctg tgctgatata aagcactacc
    21001 gtccaggccc tgatacccca tttcctcgcc atccccatga ctatcgatac ctatcccagc
    21061 ttttggatcc tcacaccagt caccacccat ttcggcacct cttgtttgcc tattggctat
    21121 ttagagagcc ggaaaaaatg ctttctacta agcatctttg tgcagcatcg ctacaaccaa
    21181 tatctttgaa aagtctcgaa ggggaaatta gtacagagca gcaatgttta cagctacttc
    21241 gaaagcaata ctcattgagt gagatttatc gccttaccgg taaaagccgc tgctacctca
    21301 ggcgacttgc cctactaaat gatattccac tcaagcttaa acccaagaaa ctgaccgggg
    21361 aatgccaaaa aaggatcatt gggttggcct acgccgggat gcacagaaag gcgatatcgg
    21421 agcggtgcgg tatcggtatt ggctcagtgg agcaggttat ttccagccag ccgggacttg
    21481 tcgaacaccg taagcggtgt cattgggagt ccaagcgtcg gcgttgtcgg cttgagattg
    21541 ccaaatatct aaaatcgcat cctggagcgc ttcgcaggga ttgcaaagct cagtgtaatg
    21601 cggctttctt ttggttatat ctcaacgatc ctatctggct ggattccgct ttaccggagc
    21661 cgatcaaacc agctgggcga tatccaaatt gttgacagcg tgaatacaaa gtcattgata
    21721 ccactcgtta tcacttcgtc gggcagcaat gacatctctg gtctgttccg acgattttgg
    21781 gatgatattt ccggcagaag aagtaacaaa tgcacttcaa caaaatagat gataaaatca
    21841 aaacgatttt agacgacttt cgcatatcaa aaaagagtct taccgatatg agcacagaac
    21901 agtgggttac tgccgagcac gttgccgagc atttgggaat cgctaaggac actgtgtatc
    21961 gatggagaga acataaagga ctgcctgcac atcgtgtggg gcggttgtgg aagtttcagt
    22021 tgtccgaagt agacgagtgg gtgcgtgcag gcggcgctgg tgaagactct ggtgaacaag
    22081 gagggcaccg agcatgattt gtacggcttt gtccgcatat gtagcggccg ttgaatagcc
    22141 agtagggaat ttgaggcaag atttatgacg accacagaac aacaaatcga actcgatctg
    22201 atcgccaagc ttggtgacct caagtacacc tatcgctccg atattcgcga tcgaaccacg
    22261 ctcgaagcca acttccgggc caaattcgag gcgcttaacc gcgtgcacct gacagacagc
    22321 gagtttcaac gcttgctcga cggcatcatc acgcccgatg tctacggcgc tgctcagcgg
    22381 cttcgcaata tcaacagctt cgagcgcgac gatggcacgc cgctgaacta caccctggtc
    22441 aacatccgag actggtgcaa aaacgacttc gaggtcgtca accagttgcg tatgaacact
    22501 gaaaatagcc atcaccgtta cgacgtgatg ctgctcatca acggtgtgcc tgtggtgcaa
    22561 atcgagctga agaccttggc agtcagccca cgccgggcca tgcagcagat cgttgattac
    22621 aaggccgatc ccggcaatgg ctacagcaaa acgctgctgt gcttcctgca gctcttcatc
    22681 gtcagcaacc gcaccgatac ctggtacttc gccaacaaca acgcacggca cttcagcttt
    22741 aacgcggatg agcgcttctt gccggtttac cagttcgcca gcgaagacaa caagaaaatc
    22801 actctgctcg atagttttgc cgagaagttc ctggccaagt gtaccttggg gcaaatgatc
    22861 agccgctaca tggtgttggt ggccagcgag cagaggctct tgatgatgcg gccctaccaa
    22921 atctatgccg tcaaagctat cgtagattgc atccatcaga attgcggaaa cggctacatc
    22981 tggcacacca cgggttcagg caagacgctg acctccttca aggcatccac cctgcttaag
    23041 gacaacccgg acatcgacaa gtgtctgttt gtggtggacc gcaaagacct ggaccgccag
    23101 acccgcgagg aattcaaccg ttttcaggaa ggctgcgtcg aagagaacac caacaccgag
    23161 accctggtgc gccgcctact gtcggatgac tacgccgata aagtcatcgt caccaccatt
    23221 cagaagctgg gcctggctct ggatggggcc aacaagcgca actacaagga acggctggag
    23281 ccattacgca accagcgcat ggtgtttatc tttgatgaat gccaccgttc acaattcggt
    23341 gacaaccaca aagctatcaa ggaattcttc cccaacgccc agctttttgg ctttaccggt
    23401 acacccatct tcgagaaaaa cgccagctac cagcaaatcg aaggtcagca ggccagctac
    23461 cgaacaaccg acgacctctt ccagcgttgc ctgcatcaat acaccattac tcacgccatc
    23521 gaggatcgca acgtcctgcg ctttcacgtg gattacttca agcccgaagg caagaatcct
    23581 cccaaacctg gtgaaggcgt ggccaagccc aaggtcattg aaaccattct cgccaaacac
    23641 gatgcggcga ccaatggccg caagttcaac acggtgttgg caacggccag catcaacgat
    23701 gccatcgagt acttcgaact gttcgcggag attcaaaaac aaaagaccga gcaagactcg
    23761 gagttccgcc cactgaacgt tgcctgcgta ttctctccac ctgccgaggg caacaaggat
    23821 gtacaacaga ttcaggaaga cttgccgcaa gagaaagaag ataaccagca agaccccgat
    23881 ggcaagaagg cagcgctcac gcgcatcgtg accgattaca acacgcgctt tggcaccaac
    23941 catcgcatta gcgagttcga cctgtactac caggatgtgc aaaagcgcat caaggatcag
    24001 cagtatccca acagcgatct ccccgccgcg caaaaggtcg acatcaccat cgtggtggac
    24061 atgctgctaa ccggtttcga ctccaagtac cttaatacct tgtacgtgga taagaacctc
    24121 aagcatcacg gcttgatcca ggcgttttcg cgcaccaatc gcgtgctgaa cgacagcaag
    24181 ccctatggca acatcctcga cttccgtcag cagcaaaagc cggtcgaaga ggcgattgca
    24241 ctgttctccg gggagaaaat cgacaaccct cgtgaaatct ggctggtaga ccccgcgccc
    24301 aaggtcatcg acaacctgca aaccgccacc cagaagctgg ctgacttcat gcagtcgcaa
    24361 ggcgtgggca atgcgccgga agaagtagcc aacctgaaag gtgatgctgc acgggcgcag
    24421 ttcgtcaatc tgttcaagga agtgcagcgt ctcaataccc agctcgatca atacaccgat
    24481 ctttcggatg agcagaaggc gcagatcacc cagattgcgc cacccgataa gatgcaaggc
    24541 ttcaagggta tgtacctgga agcggccaag cgtctgaagg aacagcagga tcgcgaccag
    24601 accccgcctg aagtgcagca gcttgatttc gaattcgtac tcttcgcctc aagtgtgatc
    24661 gactacgact acatcatggg cctcattgcc aagctgactc agcagaagcc cggcaagctc
    24721 accatgaacc gtgagcagct catcggcctg atccagtccg acgccaagtt tatcgacgag
    24781 cgtgaggaca ttgccgagta catccgaggg ctgccggtca acgtagcact ggacgaaaag
    24841 caaatccgca gcggattcga gcgtttcaaa gcagagaaaa aagcgcggga actggccact
    24901 atggctgaaa agcacgggct ggatgtggta gcgctgcaag catttgtcga tgatattctg
    24961 cgccgccaca ttttcgatgg cgaacgcctc tcggagctga tggccccgct ggaacttggc
    25021 tggaaagccc gcacacagaa ggagctggcc ctgatggaag atctgacgcc actgctgcac
    25081 aagctccaag gtcgagagat cgccggtctg gctgcctatg aggaggagag ataatgggca
    25141 ccgtctttgc acaaggggcg cagtgggttc gtgcggactt ccatctgcac acccgcgcgg
    25201 atcgagagtt caagtacaca ggcgatgaca acttctacaa cagcaattat gtggacgcgc
    25261 tggagaaagc aggtatccat ctggctgtca tcaccaatca caacaagttc gatttcgagg
    25321 agttcaaagc gctgcgcaag acagcgcaga agaaggatat tgcgctgttg cccggcgtcg
    25381 agctgtcggt caatgacggg gccaatggca tccacaccct ggttgttttt tccgacgact
    25441 ggttggccga tggccatgac catattaacc ccttcctggg cgttgcgttt gaaggaaaag
    25501 ttcctgccca gtacgagcag gaaaacgggc gttcctctct gtcgctattg gaaaccatca
    25561 agaagctgga aagctatcat cgcgactttt tccttgtctt tgcccatgtc gaagcaccga
    25621 gcgggctgtg ggccgaactg gacgggggac gtcttgccga gctgggccag aacgaattct
    25681 tccgccgccg cacgctgggc ttccagaagg tacgcacgta caacaagcag caagagaaga
    25741 gcaaaccctg ccggaccaaa acgcagcagc atctagggga ctggtacccc gccgaactgg
    25801 aaggctgcga cgccaaatgc atagaggaca tcggtcaggg caaagcctgt tacctcaaat
    25861 tgggcgagct gtcctttgaa gctgtcaagt ttgccttgag cgatcctgct tcacgcgtcg
    25921 ccatggagcc ccccaagcat caggggtcgt ttatccgcgg tattcacttc gacggtggca
    25981 tcctcgacgg aaaaacgctg catttctcgc ccgaactgaa cacgctgatc ggcatccggg
    26041 gtagcggcaa gtcatccatt ctggaagcag tgcgctacgc cctcgatatt cctcgcgggg
    26101 agaaggcgca ggataccaag tacaaggatg aactgatccg ccataccctg ggcagcggcg
    26161 gcaaggtgac gctgacggcc tgcgacgtat acggacagga attcaccatc tcccgcatct
    26221 tccgcgaagc gccgaacgtc tacctagacg gcaagttgca gcccggtgtg tcgatccggg
    26281 agaccgtgct gcgccgcccg atctacttcg ggcagaaaga cctttccagc acaggtgaag
    26341 gtttcgagac cgatctggtg gaaaagctgg tgggtgaaaa gctgcgcatg ctgcgagacg
    26401 aaatcgaaac ccaacgccaa catgtgcgtg atgcagcaag ccgctggctc aagctcagca
    26461 ataccgccga actcaggcgt gatcacgagt cccagctcac ggatgccaac ttccggctga
    26521 agaagtttga ggagtatggg gttgccgaca aactacaaaa gcgccttggc tatcagcaag
    26581 atgccactgc gctgacccgc atgaaggatc gggcagacag cttcgtaatc gccctcgggc
    26641 aattgatttc cgagcatgag gacgatctgc gtaacgccac gagctacgtc tccaagcaga
    26701 atcccggctt cttcacagct tattacgccg aatttgccaa cctcgttgcc aaggtcgatc
    26761 agctcaagaa aattgagcag gaggctcgcg ccattaccgc acggcttcaa gccaaacaag
    26821 gcgagttcga cgacgcgcgc cagagtcttc aggaagagtt tgcccaagtc gaacgtcaac
    26881 tggctcagga actcaagcag accggcatga cggcaatcca gccggatgac ttcctgactc
    26941 aacagcagcg caaaaccaag gcggaacaaa tgctggaggc gctggccaag caagaaaccc
    27001 agcaaagcag catccgcgat gtgctgtttg ccgagatcga caagctcaac gggctgtggc
    27061 tgcgcgagtt cagcgccatc aaggccgaac tggatcgcgt gaacgccggc cacaccgccc
    27121 tgcagatcga agccgacttc aagggcgaca aggaagccgc catcggcttc atgcagcagc
    27181 tcttcaaggg cagcaacatc cgcgaaacca ccctgcgcgc cgtgatggag gattacgccg
    27241 atttcggtgg cctgctgcgt gatttgccaa acgccttgaa aaaagcgggc agcacgccgg
    27301 aggtcttcga aaagaccttc atgcagaacc tagtggagtt cgtcacctgt caggtgccca
    27361 accgcttcgt catccgctat cgcggcaagg agctcaagca ccactcgctc gggcagcgtg
    27421 cttccgcgct gctgctgtac gtactcagcc agcgccagaa cgacgtgatc atcatcgacc
    27481 agccggaaga cgatctggac aaccagacca tctacgacga tgtgatcaaa ctgctgcgcg
    27541 agatgaaacc gcacgcccaa ttcatcttcg ctacccacaa cgccaacttt ccggtgctgg
    27601 gtgatgcgga gcaagtgcat gcctgccgct atcaggacga gcaagtcgcc gtacaaagcg
    27661 gcagtatcga cgctcgcccg gtgcaggacg ctatcatcaa catcatggaa ggaggccagg
    27721 aagccttcaa ccgacgcaaa gaggtttata acttatggaa accacagagc tgatcgaggc
    27781 gttaagccgt ggcgaagaca gccgccatca gttcaaagag agcttcacta atgcagacgc
    27841 actggcggcg gagatcgtcg ccttcagcaa taccgctggc gggcagattt tcattggcgc
    27901 gcaaaacgac ggcacggtgt tgggcttgtc tcacgcggac atcgaccgcc tgaatcagct
    27961 catctcgaac agcgcttcac aaaacgtgcg gcctgcggtg aacccgctca ccgaaaacgt
    28021 gccgcacccc aatggcaccg tgatggtggt cactgttgcg gaaggcgtca gcaaacccta
    28081 catggacaag aacggtgtca tctgggtaaa gaacggctcg gacaagcgcc gcgccacctc
    28141 gcgtgaagaa atccagcgcc tgttccagca ggcgggtctc gtgcatgcgg atgagacgcc
    28201 cgtggcgggc ttgggcgcgg gcgatgtgga cctgccctat tttgaaacct ttttcgaaca
    28261 gcaattcggt gaggcgctgg cccagcacaa gcaaccgctg ccgcagctcc tgaccaacat
    28321 gaacctgatg aaccaggggc tgctcaacgt ggcgggcagt ctcttgtttg ccaaagcacc
    28381 gcagtatgcg ctacctgcct tcatcgtcaa ggcggtggcc tttgtcggca tcgacatcga
    28441 ggatcagcgc tatattgaca gccgcgatat caccggcaag ctggctgatg tgttccagca
    28501 gacactgggc tttatcgttg ccaacacccg cgccactcag ggtgaacagg gctttaactc
    28561 acaggggcag gccgagattc cgcgcattgt ctgggaggag ttggtcgcca acgctctcat
    28621 ccaccgcgac tacttcatca gctcgccggt gcgcgtgatg gtgtttgccg atcgcgtcga
    28681 gatcatcagt cctggccatc tgcctaacaa cctgaccatc gaaaacatca aggcgggcaa
    28741 ctccaatatg cgcaacccga ttctggcgtc ctttgccgcc aagctgctgc cgtatcgcgg
    28801 actaggcagc ggattgctac gcgctcttcg ggcttggccg cagattgaac tcatcgacga
    28861 ccgggctggg aacttgttta aggccatcgt gacacgacca caggtgcaag cggcatgagt
    28921 ggtaatgaaa aacaacagct ggtgccacgg ttgcggtttc cggagttcaa gaatacagcc
    28981 gagtggcaaa gtcctacatt gggcgaagta tctgaaccgg ttgaagaacg ggtgggcgag
    29041 cgaaaactca cgccggtgag tatctctgcc ggaattggtt ttgtaccaca ggcagagaaa
    29101 tttggacggg atatttccgg gagccaatac aagctatata ccgtagtgcg tgatggtgac
    29161 tttgtttata acaaggggaa ctctctcaag ttcccacaag gttgcgtata cgatcttcaa
    29221 ggttggggtg aagttgcagc acctaatgtt tttatctgct ttcgcttgaa aaatggatac
    29281 gagaatggct tttatcgcca atgctttgag cggaatacgc acggcatcca gctgagaaag
    29341 cacatcacaa gcggagcaag gagcaatgga ctgctcaata tcagtaagga agctttttat
    29401 agcgtggcga ttccagtacc aagccatgca gaacaacaaa aaatcgccga ctgcttgtct
    29461 tcgcttgatg ctctgatcgc agcagaaatc caaaagctcg gcacccttaa gacccacaaa
    29521 aagggcttga tgcagcagtt gttcccccgc gaaggcgaaa ctgttccacg actacgtttt
    29581 ccagagtttc gagatgatga tgggtgggaa tctgacttac ttggaaatat tttcgataca
    29641 acctcaggcg gcacaccaag tcgttcaaca aaggaatact gggatggtta tattccttgg
    29701 atcacaactt cgttggttaa ttttggcatc attacccatg cggatgagtt tatcagtgag
    29761 gcgggattaa agaattccgc agcaaaactt ttccctcgag gcacggttct cattgcgatg
    29821 tatgggcaag gaaagacccg aggtcaagtg gcattactcg gtattgaagc agcaaccaat
    29881 caggcatgtg ctgctatact gcctcgaaaa gaaatcgatc ctaactttgt gtttttaaat
    29941 ttggcaagtc gatatgatga gattcggcaa ctgagtaata gcggtgggca ggaaaatctg
    30001 agccaaggtc tgattcgtga gctttctttc tcctatccgt taaaccctgc agagcagcag
    30061 aagattaccg cctgcctaac ctccgtcgac aatgtcataa atgcacaagt ccagaaaata
    30121 gagacattaa tatccaataa aaatggccta acgcagcaac ttttcccctt ccttggtgag
    30181 gtttcatcat gaagcctggt cagaccttcg ctgaccttct aaggctggct gctcatctgc
    30241 gtggtgatct ggagaacaag aaagccatcc tactctatgc ctacaacggt acgggcaaga
    30301 cgcggctatc catgaccttt aaggacatgg gcaagcaggc cgaggcccgc gataccctgt
    30361 atttcaacgc cttcaccgaa gacctattcc actggaacaa tgatctggta ggcgatgagg
    30421 accggcgttt gctgctcaat agggactcgc gtttttttca ggggctgttc gatctggaga
    30481 tggacaaccg tatccgccca ttgttgcggc gttacgcgga ttttgacttc cgtatcgata
    30541 cagagggccc agagtgggcg gtacgctttt cgcgcttagt ggatggtcag cccgtcgaca
    30601 acatcaaggt gtcacgcggc gaggaaaaca tcttcatctg gtgtttcttt ttggccgtgg
    30661 tagaactagc gatggacgcc gatatcgagg cttaccagtg ggtgaagtac ttgtatatcg
    30721 acgatccgat ttcgtcgctg gatgaacaca acgccattgc tgtcgccaat cacttggcgc
    30781 agttgctcaa gcggcaggac aaccggctca agacagtgat ttccacgcac cacaccttgt
    30841 ttttcaatgt gctctgcaat gaactgggta aggcgcgccg ctatgtggtc aacaagcact
    30901 cggccaacgg cggctatctg ctgcgacccg aagagggcga tacgcccttc tttcaccatg
    30961 ttgccgcttt ggctgaactg tatcaggccg cgcaggaaga tcgtttgttc acgcaccatt
    31021 tcaatatgct gcgcacgatt cttgagaaga cggcgagttt ccacggccac aaaaactttt
    31081 cggtttgcat caagcaagac gatgacgacc cggacggcat tttgcatgcg cgcctgatca
    31141 acatcctaag tcacggcaac tattcgttgt atgagccgca gcagatgctc gatgagaaca
    31201 aggcttactt caagaagatc ctgaacgagt tcctgaatcg ctatcccttc aatcccgatt
    31261 tgttccctca agttcctgag gccgcaacag caggtacacc atgactgaat tcgaaaaaca
    31321 aaaactgggc aaaatcctct gggccatcgc cgataaactg cgtggcgcta tgaacgcgga
    31381 cgatttccgc gattacatgc tggccttcct cttccttcgc tatctgtcgg acaactacga
    31441 ggcggcggct cagaaggagt tggggtcaga ctacccgcga cagattgacg gttcggtctc
    31501 cacgccactg cagctttggt acgaaagcaa ctacgaagat gtgccagaat tcgagaagca
    31561 gatgcgccgc aaggtgcatt acgtgatcga accccagtat ctgtggggta atatcgccga
    31621 aatggcgcgc aaacaggatg acgagctgct gcgtacctta caaaaggggt tcaagtacat
    31681 tgaggaagag tccttcgcca gcaccttccg gggcctgttc tcggagatca atctggcgtc
    31741 tgacaagctg ggcaagacct acatcgagcg caacgcccgc ctgtgcaaga tcatcgcaga
    31801 gatcgccaag gggctggtgc agttctctac cgatagcgac actcttggcg atgcctatga
    31861 gtacctgatc ggccagtttg cagcaggttc gggcaaaaag gccggcgagt tctacacgcc
    31921 gcagcgtatt tcagacatct tgtcggccat cgtcacgctg gacagtcagg agcctgccac
    31981 gggcaagcgc tcgcatctgg atagtgtttt cgactttgcc tgcggctcag gctccttgct
    32041 gctaaacgtg cgccgcctga tggggccgca cggcatcggc aagattcacg ggcaagagaa
    32101 gaacatcacc acctacaacc tggcgcgcat gaacatgctg ctgcacggtg tgaaggactc
    32161 ggagttcgat atcttccacg gcgacactct attcaacgag tgggacatgt tgcgcgagac
    32221 caatccggct aagatgccga ggttcgacgc tgtggtggct aacccgccgt ttagctatcg
    32281 ctgggagccg agtgaggcgc ttggcgaaga cgtgcgcttc aagaattacg gcctggcacc
    32341 aaagtccgcc gctgactttg ccttcctgct acatggcttc catttcctga agcaagacgg
    32401 tgtgatggcg atcatcctgc cccacggtgt gctgttccga ggaggtaccg aagcgcgtat
    32461 ccgtaccaag ttgctgaaag acggacacat cgatacggtg attggcctgc ccgcgaacct
    32521 gttcttctcc acaggcatcc cggtgtgcat cttggtgctt aagaagtgca agaagccaga
    32581 cgatgtgttg ttcatcaacg ccgccgagca ttatgagaag ggtaaacgcc agagccagtt
    32641 gctcaggacg gacgagatga cggatggtgg aatcgggcat attgaaaaga tcatcgatac
    32701 ctatcggtat cgaaaggaag agcctcgcta ctcgcgccgg gttagcatgg atgagatcga
    32761 aaagaacgat ttcaacctga acatctcgcg ctatgtgagc acggtgcaag ccgaggaaga
    32821 gatcgacctg gcagctgtac acactgactt gctcgttgtg gagaagcaaa ttggtgcggc
    32881 aaaagaacgg tacaaccaat acttagcaga attgggtctt cctttactac cttaactttt
    32941 ttgaaagggt ttgcatcgtg agaaacttgc gttttatgca tcatgcaaac ccaattatca
    33001 ggcttattcc gaccctacgc atttagatgc tattggtagt agataagcta acagtagctt
    33061 gggttccttt cgtttttaga aacatctcaa acgagtcaat aaattgatta attaatgaat
    33121 caggctagtg gtatatgtcc ccaatcggat gagtcgcttc agagctttat tcataggatg
    33181 atgcttcgtg ttggctggag cgattttaca acccttataa ctcatggagg atgggggagt
    33241 aatccgtctg ttccatacga ggtgaaacag gaattcgatg catttgatgc ttccatactc
    33301 ttggatctct ttgaaaacca gtattatgca aataaaaatt acagcttatt tgatggaaga
    33361 ttttcacata taagcctatt ccaggaaacc tttttccctc ataagaagaa aaccttttgc
    33421 ggaagcagag taccattacg tttctgtaga gcctgcatag aaaaacaact tcacgaaagg
    33481 gggttctcgt attttaagtt ggattggttg tacgaagcct tctgtgaaat tcatgcgaca
    33541 cctctactca aagttaagga aagcttatct ctttcaaaaa ttaaaaacac attgaagttt
    33601 gtttttctgg ctgattggca tgaaatttta gatgtggttt cagaagaggt gttgctggag
    33661 gatgaacatc ataaaatcga ggagtcagat ttaacaagat ataaccgaat gctaagcttc
    33721 tctccttgcg ccaaaagggg gctggtcgaa tactttatga gtagttctga ttattaccca
    33781 acaggttgct cttcaattgc tgattatggt tttctctcca aaatccagag agacctcctg
    33841 tttaaaaaaa ggaaaaggga ttcgttacaa agtcaccttg aggaagctta tgaatatgaa
    33901 ttgagtaata attatcactc attaattgag cattgcatta aagatcttga gatcatggct
    33961 attcaccatt ttggtaaagg acactgggta attaaatcta ctagtagaaa atgtgtagac
    34021 tgcttaagac acaacgtcga cgatttcagg tcttgcccag cggctggtat tatcagttgc
    34081 acaggtggat atttatcatc ggcagagaat caatgcgata aatcactgga tagtctggaa
    34141 tcagatgttg aattacatca aaggcgattg aaagtaaccg atggtgagcg gtttgtgcga
    34201 gacagtattg agaaaagtag ggctattact gccgcaggag gaccgcaagc ttatcgcaga
    34261 gaacgtgaag cgcgcctaaa ggcggctgcg aaagcgttgg accccctgat ttcgtaggaa
    34321 tcttcgcact gctacaatag cggaaacgcc catctgaaca aaaaacgacc aaaaggtgat
    34381 tctatggcca gaacattgga agaacttctc cagcaggaga aacctgaggt ggttgaagca
    34441 gcaaaagata aagctgaaaa aattctcagg gagcttgagg gtgtagatca ggacactccc
    34501 caagacgagc ttgatgaacc ggctgataac cactcctccg gatcaagtta gtgtacaaac
    34561 tttattgttc caagcaagga tctagtgtac aaactttatt gtcgctgcac aattgctgtc
    34621 cgaggacaat aaagttgtac acaaagcact aaagttgtaa ccattctcaa aaaacgacgc
    34681 taatgttgta tacttactga atgtgtggtt atagggttgg agactataaa atacacatat
    34741 aggtacgtat caatgtcgtt tttagggtac gatcatggct aggggtaggc gtctcaaatc
    34801 ctatttggat tatgaaaatg cgctaggtga cggcatagga gtgggctatg gccaaagtta
    34861 tcagccctgg cttagagctc aggacgttaa atcccgtgga aaccgttcga tagtctttgg
    34921 ccttaagacg tttcgaaacc atcatctcct ttcttctgtc gaaagtaact ttttctatct
    34981 ggctgagttt aatgactcgg tgattgatat ccgggaacaa ttcccactct ttcctctccg
    35041 gcttacccaa caaatagcaa atcatctaca ttttcaacat cctatggtga ggggagtaag
    35101 aggagtacct gtcgaagttc tgaatgttat gacaaccgat tttttactga ccttgagaac
    35161 tcctgaaggc ggacttcgat acaaagctat agcagtaaaa cataacgaga gcatacctga
    35221 acgcgaagcc caaaaacttg aaatagagag gatgttttgg cagttgattg atgttgagtt
    35281 tcaaatttat gttggctcgg aactcaataa cgtcgtcggt aaaaacattt gctgggctac
    35341 ttctgtatta agagatggtt ctgaatttta tgataaatat cctcttgata aaatcctatg
    35401 gaagcttaaa ccagatgttt atcccatagt aggactacgt gcaatgattt catcaatctt
    35461 tggggtagat gcacaagaag cgatgatgtt attgcaggca atgattggat taaaaatgat
    35521 aaatgttgat ttatcatatc caatactcga aaccggtctg ataaagataa tttccaatga
    35581 ccactatata ggactgaacg caaatggata ttattagaaa ttcagtctgg ctctctcaag
    35641 gcactgattt gcttgcagag ggactttacc gtgttttgga ttttgacaga aaggtcgatt
    35701 tgttaatttt gtttaaaata aaatcagaaa gaacgggtaa gccaatccct ttc
//
LOCUS       DAFNBJ010000031        35617 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000031 DAFNBJ010000000
VERSION     DAFNBJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.031"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(51..1910)
                     /gene="gss"
                     /locus_tag="L2X49_001493"
     CDS             complement(51..1910)
                     /gene="gss"
                     /locus_tag="L2X49_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBN4796599.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDLLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2115..2981
                     /gene="yghU"
                     /locus_tag="L2X49_001494"
     CDS             2115..2981
                     /gene="yghU"
                     /locus_tag="L2X49_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBN4796600.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3104..3352)
                     /gene="hybG"
                     /locus_tag="L2X49_001495"
     CDS             complement(3104..3352)
                     /gene="hybG"
                     /locus_tag="L2X49_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBN4796601.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3365..3706)
                     /gene="hypA"
                     /locus_tag="L2X49_001496"
     CDS             complement(3365..3706)
                     /gene="hypA"
                     /locus_tag="L2X49_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBN4796602.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3699..4187)
                     /gene="hybE"
                     /locus_tag="L2X49_001497"
     CDS             complement(3699..4187)
                     /gene="hybE"
                     /locus_tag="L2X49_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBN4796603.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4180..4674)
                     /locus_tag="L2X49_001498"
     CDS             complement(4180..4674)
                     /locus_tag="L2X49_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBN4796604.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4674..6377)
                     /gene="hybC"
                     /locus_tag="L2X49_001499"
     CDS             complement(4674..6377)
                     /gene="hybC"
                     /locus_tag="L2X49_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBN4796605.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6374..7552)
                     /gene="hybB"
                     /locus_tag="L2X49_001500"
     CDS             complement(6374..7552)
                     /gene="hybB"
                     /locus_tag="L2X49_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBN4796606.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7542..8528)
                     /gene="hybA"
                     /locus_tag="L2X49_001501"
     CDS             complement(7542..8528)
                     /gene="hybA"
                     /locus_tag="L2X49_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBN4796607.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8531..9649)
                     /gene="hybO"
                     /locus_tag="L2X49_001502"
     CDS             complement(8531..9649)
                     /gene="hybO"
                     /locus_tag="L2X49_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBN4796608.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9838..10125)
                     /locus_tag="L2X49_001503"
     CDS             complement(9838..10125)
                     /locus_tag="L2X49_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBN4796609.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10244..11131)
                     /locus_tag="L2X49_001504"
     CDS             complement(10244..11131)
                     /locus_tag="L2X49_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBN4796610.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11288..12328
                     /gene="gpr"
                     /locus_tag="L2X49_001505"
     CDS             11288..12328
                     /gene="gpr"
                     /locus_tag="L2X49_001505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBN4796611.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12368..12862)
                     /locus_tag="L2X49_001506"
     CDS             complement(12368..12862)
                     /locus_tag="L2X49_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBN4796612.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13053..13937
                     /gene="yghA"
                     /locus_tag="L2X49_001507"
     CDS             13053..13937
                     /gene="yghA"
                     /locus_tag="L2X49_001507"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBN4796613.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14209..14634)
                     /gene="exbD"
                     /locus_tag="L2X49_001508"
     CDS             complement(14209..14634)
                     /gene="exbD"
                     /locus_tag="L2X49_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBN4796614.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14641..15375)
                     /gene="exbB"
                     /locus_tag="L2X49_001509"
     CDS             complement(14641..15375)
                     /gene="exbB"
                     /locus_tag="L2X49_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBN4796615.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15627..16814
                     /gene="metC"
                     /locus_tag="L2X49_001510"
     CDS             15627..16814
                     /gene="metC"
                     /locus_tag="L2X49_001510"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBN4796616.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16954..17613
                     /locus_tag="L2X49_001511"
     CDS             16954..17613
                     /locus_tag="L2X49_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBN4796617.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17653..18552)
                     /gene="yqhC"
                     /locus_tag="L2X49_001512"
     CDS             complement(17653..18552)
                     /gene="yqhC"
                     /locus_tag="L2X49_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBN4796618.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18746..19909
                     /gene="yqhD"
                     /locus_tag="L2X49_001513"
     CDS             18746..19909
                     /gene="yqhD"
                     /locus_tag="L2X49_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBN4796619.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWGATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20014..20841
                     /gene="dkgA"
                     /locus_tag="L2X49_001514"
     CDS             20014..20841
                     /gene="dkgA"
                     /locus_tag="L2X49_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBN4796620.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYIN
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21041..21967
                     /locus_tag="L2X49_001515"
     CDS             21041..21967
                     /locus_tag="L2X49_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBN4796621.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22018..22275
                     /gene="yqhH"
                     /locus_tag="L2X49_001516"
     CDS             22018..22275
                     /gene="yqhH"
                     /locus_tag="L2X49_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBN4796622.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22318..24537)
                     /locus_tag="L2X49_001517"
     CDS             complement(22318..24537)
                     /locus_tag="L2X49_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBN4796623.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24648..26060)
                     /gene="ftsP"
                     /locus_tag="L2X49_001518"
     CDS             complement(24648..26060)
                     /gene="ftsP"
                     /locus_tag="L2X49_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBN4796624.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26135..26872)
                     /gene="plsC"
                     /locus_tag="L2X49_001519"
                     /gene_synonym="parF"
     CDS             complement(26135..26872)
                     /gene="plsC"
                     /locus_tag="L2X49_001519"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBN4796625.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27106..29364)
                     /gene="parC"
                     /locus_tag="L2X49_001520"
     CDS             complement(27106..29364)
                     /gene="parC"
                     /locus_tag="L2X49_001520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBN4796626.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29502..31109)
                     /locus_tag="L2X49_001521"
     CDS             complement(29502..31109)
                     /locus_tag="L2X49_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN4796627.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVVGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31242..31637)
                     /gene="mqsA"
                     /locus_tag="L2X49_001522"
     CDS             complement(31242..31637)
                     /gene="mqsA"
                     /locus_tag="L2X49_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HBN4796628.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            complement(31639..31935)
                     /gene="mqsR"
                     /locus_tag="L2X49_001523"
     CDS             complement(31639..31935)
                     /gene="mqsR"
                     /locus_tag="L2X49_001523"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HBN4796629.1"
                     /translation="MEKRTPHTRLSQVKKLVNTGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            complement(32140..32622)
                     /locus_tag="L2X49_001524"
     CDS             complement(32140..32622)
                     /locus_tag="L2X49_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN4796630.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32675..33067)
                     /gene="ygiW"
                     /locus_tag="L2X49_001525"
     CDS             complement(32675..33067)
                     /gene="ygiW"
                     /locus_tag="L2X49_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBN4796631.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33219..33878
                     /gene="qseB"
                     /locus_tag="L2X49_001526"
     CDS             33219..33878
                     /gene="qseB"
                     /locus_tag="L2X49_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBN4796632.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33875..35224
                     /gene="qseC"
                     /locus_tag="L2X49_001527"
     CDS             33875..35224
                     /gene="qseC"
                     /locus_tag="L2X49_001527"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBN4796633.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPPEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(35270..35602)
                     /locus_tag="L2X49_001528"
     CDS             complement(35270..35602)
                     /locus_tag="L2X49_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBN4796634.1"
                     /translation="MLKKQLLSIFEALLYIMFTYWLIDAFFAFNKYDWMLDSGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
BASE COUNT         8775 a         9469 c         8944 g         8429 t
ORIGIN      
        1 ccccgtactg attattcttc ataatcagta cggggttgct acaacatgta ttactttttc
       61 accacaatta acggttcaat atcactctct tttttaataa ccagtgattc atcaccgcgc
      121 aaacacgtcc caccgtagtt gccgccaacg gtgaaggtac atacctgaat gtatttaccg
      181 tccactttcg gcaaacacca cagttgctga tagatgtttt tctgctcggc aaatttaccg
      241 ctggttttgt ccagcacctc ttcatgatgg ctgacgaggt cgatattgct gccacagcga
      301 ccggcgatcg gtttcactgc gtaacctgtt ttcaccagtt catcattaac agtgaaatcg
      361 gtatccagca ggtaacgatg gtgcgggaac agcgaccaga ggatcggcag aatcgctttg
      421 ttgccgggga tcaccgtcca cagcggctca aagaccagca cttccgggcg cagcaatacg
      481 tcgataagac gcacttcgtt ttgcggatga ccggtacgga ttggcaccgc agcaaactca
      541 cggtcgctaa cttcacgaat ctgatcaaac gcggtttccc acgcccaggt tttccacacg
      601 cagttaacca gccgtccttc cccatcaatc agttgcccgg cagcatccca gcccagttca
      661 tccagcccgc gcaagatacg cgtttcaaag cccgcctggt gcagcgcctg ctccataaac
      721 tgcgcgtgat agttttcctc gatatctttg tcctgcatga tatggacaaa cggacgtgca
      781 cgactgtgtt tccaggcacc agccagttcg ttaatcagcc cttccgccgg attgaagccg
      841 ttgcctttat agccctgctc cgcccaacgt tcgaggatca agcccgcttc ggtatgacat
      901 gaggcagagt cggcgttgta ttcgtaaacc ttcaggccac gctcatccat gcagaaatcc
      961 atacgaccag tgatcatatg gtgacggcga cgctgccagg agagacgcaa acgtggccag
     1021 aggattttcg ggatgtcgaa cagcgccagc aggttgtcat ctttcagcac cttgtcggtt
     1081 gcgtgaagat acatcaggtg cagctcgttg gtggctttaa ttagctcctg ctcggcactc
     1141 tcggtaatgg tgtagtaatg ataaggatcc tgattgatca cctgaccgtt ggcctgcaca
     1201 taggcgtttt gcagcagatc tttttcatcc agccatttac cgtcaaactg gcctttgttt
     1261 tccaggcgcg ctccgctgat tttcagcagc tcgcctgcaa tttccggctg cggtaagctg
     1321 tattcagtat cttccgtctg gatcatccag cccagaatgg tggtgtcatc aaaagtgtct
     1381 ttcagggtat agcagccgtt ttcgaccacc atctccagct cacgcgtcca ctgttgccct
     1441 tgcggcaacg gggaatgaat cacgttctgt tccgcaatac ggactttgtt gccatgcaat
     1501 tgggtaatga tggcgacatg gccagtgtct ttaaattcac cgcctttatc ccagataaga
     1561 agcgcacccg cgaccggcgc acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg
     1621 ttgtcattaa ccacttctcg caggaagcgc agcgagaaaa tctcccacgc catacccacg
     1681 tcagtaaaga ccacaccgta attcagaaag agaaaacggc gagcaaattc aacgcattgc
     1741 cacttgtggc ccatatattc gtcgtcgata tagctacgga atacggcgtc atcttcgtat
     1801 tcctgcggat cgagagaact gtaatctgaa gagtagattg ctaccccacc tggggcgtag
     1861 cccaataatg tcccgaacgg ggcatcctgg ctggtcgttc ctttgctcat cactttacct
     1921 ttaacaatac aacctaagca gttggcgtaa tagttgtgct ctgattacct gctcagcgac
     1981 attaaccgga cagaggtcag actaatcata cacctcatcg cagcggctgg cgcaaagggt
     2041 taaaaaattc acattctgta cagcaagtga cctgctacac tgcttcaaca ctaccactca
     2101 gaaggcaact cactatgaca gacaatactt atcagcccgc gaaagtctgg acgtgggata
     2161 aatccgctgg cggcgcgttc gccaatatca atcgcccggt ttctggtccg acgcatgaaa
     2221 aaacgctgcc cgttggcaaa cacccattgc aactttattc gctgggaacg ccgaacggtc
     2281 agaaagtaac gattatgctt gaggagctgc tggcgctggg cgttactggt gcagagtacg
     2341 acgcctggct gattcgtatt ggcgatggcg atcaattctc cagcggcttt gtcgaagtga
     2401 acccaaactc gaagatcccg gcgctgcgcg atcatacgca taatccgccg atccgcgtgt
     2461 ttgaatctgg ttcgatcctg ctttatctgg cggagaaatt tggctacttc ctgccgcagg
     2521 atttggcaaa gcgtactgaa acgatgaact ggctgttctg gttacagggc gcggcaccgt
     2581 tcctcggcgg tggttttggt cacttttacc attacgcacc ggtaaagatt gagtacgcca
     2641 tcaaccgctt taccatggaa gccaaacgtc tgctcgacgt gctggataag caactggcgc
     2701 agcataagtt tgttgcgggc gatgagtaca ccattgcgga tatggcgatt tggccgtggt
     2761 ttggcaacgt ggtgttaggt ggtgtgtatg atgccgctga gtttcttgat gcgggcagtt
     2821 ataagcatgt acaacgctgg gcgaaagaag taggcgaacg tccggcggtg aaacgtgggc
     2881 gtattgttaa ccgcaccaac ggaccgctga atgagcagtt gcatgagcgc catgacgcca
     2941 gtgatttcga gacgaatacg gaagataagc gtcaggggta agggttggtg ttcgtcgcag
     3001 caagccatcc aggccggata aggcgttcac gccgcatccg gcaatcgtgc acaatgcctg
     3061 atgcgacgct gccgcgtctt atcaggccta cgaaagctaa tcatcacgcg ctggtaatgt
     3121 cgtaatccat ttggcgcagt gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc
     3181 tcatggcaaa tccgacgtgt accagcaccc actggcccag tagatcggca gggttacctt
     3241 cacaaatcag ggcgatattc acgtcgcgct tgataccaca tacttcaacc tgcgcaagct
     3301 ggtgaatatc ttcaccgaca gccagcacct ggcctggaac gccaatacac attattaact
     3361 ccggttattc aacttcaata cttttgacga tcagcgaatc gccggtatcg acacgcaacc
     3421 gctcgccgtg acagagcgga cactgcgcat cgtgctgatg aatctccacc acctggctgc
     3481 aatcccagca ccaggcctgg gcgggtttat agacgatatg taaatcgcac ccttgcgcca
     3541 ccgttccgtg gcagacaatt tcaaaactaa aacggacggc gctctcctca acgcaggaga
     3601 gcgcgccaat ttccagccac acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc
     3661 gttggataat ttcaacggcg ctctggcaaa gagacaactc atgcattttc gccactcctg
     3721 cgaccaaaca gcagggcgcg acgccctgcg tgtggtacat ccggattcgt gactggcagc
     3781 gaaaggatca ttcgtgcgca gtcatccgtc aggcgttggc cctcttcaat cgacatgctg
     3841 tgcgaaagcg gcgacatcag cgaacaggag agatattgcg aaacaccgtc cagttcgcca
     3901 acggtaaagg tcatagtgcc atacggcagt tgcagaccaa ttttttcact cactttgcgc
     3961 agcggccaga gttgatccgg gccggggaag ataactgcac tcagcatcca cggggtgatc
     4021 acacaccccg tccactgacc ttcgaacagc gtaaaatcag aaacatacac cggcattgaa
     4081 ggatgcagaa aagagagatc gtgcatcgaa cgccgggcaa tttcttcaaa cgctgcctgt
     4141 acttgcgcct tcggggaggt ctggaaacct gctatctctt cagtcatgaa tcgcctcccg
     4201 tgggatggct tccacgccag attcacgcag cgcagccaga acctgctcaa gcgcaggttc
     4261 aatcattgct tcaaccgtcg gcgttaagcc gatgtgtggc tccagcgatt ccgggatcac
     4321 gccgaccagg gtcagctttt tcggaaactc accggtgaag cgcagggccg acaagacgtc
     4381 ggccaggcca agctgatgcg gagagatttt gttggtaaac aacgccggaa cttcttcatc
     4441 gcgcaggatc atcatcgttc ccggcgcgct cttcttcgac acaatggcat ccgcaataat
     4501 cagatgatcg cgatttgcca tgtcgccaag cagctccatt cccgccgtgc cgccatcgag
     4561 gatctcaaca taatccggca gaatgtatcg ttgctctaac gcttcgacaa tccgcacacc
     4621 gatggcttca tcggtcagca aaatattgcc gacccctaag actaaaatac gcattacaga
     4681 accttcactg aaaccacttc gttgccgtca gcatccacta cgtgtaccgc acaggccatg
     4741 cacgggtcga aggagtgaat ggtacgcacc acttccagcg gtttattcgg atcggcaacc
     4801 ggtgtaccca ccagcgactg ctcgtaagga ccgacgtcat cattgaagtt acgcggacca
     4861 gagttccagg ttgatggaac aaccgcctgg tagttactga tgataccgtc tttgatcacc
     4921 atccagtgag agagcatacc gcgcggcgct tcgaggaagc caacaccttt gaactcaccc
     4981 gttgccggaa tgttcggctt cacaaaggtg gtatgatcgc ctttaccgat attggtgatc
     5041 agtgcactgt attgattttg caggatatcc tgcaattcgc agcagtgaac ggtacgacca
     5101 ataatacggc ccagcgtaga gtgcagttgc gccacttcaa gcgtgttacc agtcagtttc
     5161 tgataaatcg caacgatttc attcagtttg ttctgggtag actcgcgtcc tgccgccagt
     5221 ttcaccagca tgttagccag cggacccact tctaccgttt tgccgtagaa agtcggtgat
     5281 ttcacccagg aatatttccc gtcgtcagac caaccatcat aagccggaat ggtggtacct
     5341 tcccacggtg cctgcggcgc ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg
     5401 cctttgatca ggtattcatc ggaatgagaa gtgatcggac gatacgagga cagatccgca
     5461 ttctcaatgt agccgcccgg gaacaggaag ctgccgtttt tactgtcggt cgggaattcc
     5521 ggcacgctca ggtagttcac cgcaccttta ccgcgcgtca gccattccgg gtagaacgcg
     5581 gcaataactg cggtatcaac cttataaacc tgttcaacaa agtcgctcag cttgtcgatg
     5641 aaagacttga tgtacatcag gcgctcaagg ttcagcacgc ccaaaccgtc gaggttgatc
     5701 gggttcgcga caccacctac cgccaggttc tgaatgtgcg gcgttttacc gcccagcagc
     5761 gccacgacgc ggttagcgtc acgctggcac tccagcgcct gcaggtagtg cgctaccgca
     5821 atcaggttca cttccggcgg cagtttcatc gccgggtgac cccagtagcc attggcgaaa
     5881 atacccaact gaccgctggc aaccagatct ttgatcttgt tctgaacttt ggtgaactct
     5941 tccggactgt tcaggtgcca ggtcgaaacg cctttcagca tttcggaggc tttggttggg
     6001 tcagcttgca gtgcagaagt gatgtccacc cagtccagcg ccgaaagctg atagaaatga
     6061 acaatatggt catgcgtggt gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta
     6121 accggaacgt cgatattcag cgcactttct gccgcacgaa cggaagacag cgcgtgagtg
     6181 gtagtacata cgccacagat acgttgcaca atcatccatg catcgcgcgg atcgcggttt
     6241 ttcacgatct cttccatgcc gcgccacatg gtaccggaag cccatgcttt cgaaacgacg
     6301 ccattttcga tttcgcaatc gatgcgtaaa tgcccctcaa tacgggttac cggatcaata
     6361 gtaattctct ggctcatgct ttgctcgcct catgacgatt atgatcgttt tgttttaaag
     6421 gaggaagtat cggcagtaga cgaatgagta cgatgtaagc gcaaatctca atagccacaa
     6481 aaccaataga aatcaacagt tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt
     6541 tgaatgccac cagcgaatag gtcagacgcc aggttgcaca acctaacagt gcgctcagtg
     6601 ctgacaggaa cagcatgcgg gaatcattac gcagcttcgc cacacgcaga acgaccagcg
     6661 ggaagagcat cagcaggact tcaatccaga acatcacgga gtagaagtca ccggcaaacg
     6721 ctaacgacag cttgtcgcga tagataagct cgccaaagcg cagcacgatg aaaatcgcca
     6781 gcaacacact gatggtgttg gtcagcttaa caaacagact cttttcatcc ggaccgttgc
     6841 cacgcagacc cgcctgcacc agcgaacctt caaagatgac aatcgagaag cccatgatga
     6901 acgccgtcag cagcgagaac agcggcaaca tttcatagct ctgccacagc ggatgcacct
     6961 tgtagcccgc cgagatcatc agcgacccca ttgaagactg gtgcatggtc ggcagcagcg
     7021 caccgagcgc gatgatgaag aacatcacct tgtttagtcg ctgtagcgac accttccacc
     7081 ccagacgttc aaacagtgcc ggagcaaact ccagtgccat cacgccgata tagatggtca
     7141 tacagaccgc cgtctcgaac agtaccgagt tcacgttgaa gtggcccgga atgtagaagt
     7201 acggcaggtt ccagtagcga cccacgtcga tagtgatcga caagccaccc agtgagtaac
     7261 caaacagact cgccaacagc gccggacgca ccagcggatg gtattgccca cggttaaaga
     7321 cgtataccgc ccacgccagc gcccagccgc cacaggcaaa gccggtgcca atcagcaggt
     7381 caaacgcgat ccacacgccc cacgggaagc cgccgttcag gtcagagaca gagcccagac
     7441 cgaacaccag acgcttcaca atcaggagca tacagatgac gattaacggt ccaaaaatca
     7501 tgaccggttt actgatgatt ttgccgccca gcggttgtgg atcatgactc atgatcgtct
     7561 cctccgtcgt gatggtcgtt tttggtgttg cgacgaacca gcacggttaa gcccgccagc
     7621 acagccagtg gtagcatcat gcctttatac agggtgtgtt gaatattttc ggaacgcgca
     7681 ccggtagaaa gatcgtccag tttcggcagg tcgagatttt cataaggcac acccgtcagt
     7741 accagaacct gagtaccgcc gccctctttc tcgccgtaca gatgcggata atatttcggc
     7801 actgtatgca ggtaagtgtc gccagatttc agcgtctgac gcggatagtg gtattcgctg
     7861 ccaggcttca gcgccagacg ttttttcgcc tccgccatca gctcttcacg cgtaccgaaa
     7921 atcaccgcgc ccgccgggca cacttctacg cagccaggca gaccgccttt atcgagacgt
     7981 tccacacctt tctggttgca cagctcgcac ttatgcagcg caccaaacgg gttgttgtag
     8041 tcgtacttcg gcacgttgta cggacaggcg accatgcagt aacggcagcc ggtgcacacg
     8101 tctttgtcgt aatggacaat gccggttttc ggatcttttt tcagtgcaga gaccgggcac
     8161 acagagacac agttcggatc gacgcagtgc atacactgtt tcttaatgta cgcgtagccg
     8221 ttctcctcct ggtctttgtt gacccctgtg ccgctggtcc acacctgaat gatgttattg
     8281 gtatacggcg acagtttgtc gttgttcgac caggtctgtt ccccttgcgg gttacgttca
     8341 gggaagttga tatcctgaca cttggtgacg caagcctggc agcctacgca caaggtcgag
     8401 tcgtacaaca tccccagcga tcccggaatt ggcgggcggt tttcagcagc cgcatgactg
     8461 acagacggca gcgcgcccgt cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt
     8521 ctgttcacgg ttattctccc cgtgagtcag cgttatcttt cttttgctga cgacccagtt
     8581 cacgcaccgc catcacgctg acaccggcaa cgagcccaac cacaccgccg agcaaaccaa
     8641 tagcgcctgc agagacgttg ccgccctctt tagcgttaac atccggtttc tgtgaacgcg
     8701 gagtctgatt ttcgacgttg gcaagctgat ggatgccttt atggaagccg ataccttctt
     8761 cgttacagcc atagcatggg tgaccaatcg ctaccggcca cacgccgcca acatcgcaga
     8821 attgcagcgt tgagcagttg ccgtaagttt ctggcccttt acagccgagg tggtacaggc
     8881 accagccttc acggtggcct tcatcaccga actctttggc aaaacgacca gcatcgaagt
     8941 gcgggcgacg ttcgcagtgt tcgtgaatca gacggccata ggcgaaggtc ggacgatttt
     9001 tgtcatccag tttcggcggt ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt
     9061 tgtgcgggtt cggcgggcag cccggaatat tgataacggt tttgcctggc agaacttctt
     9121 gcaggctgac tgcgccagtt gggttaactc cagctgcggc aacaccgccc cacgcagagc
     9181 aggaaccgat agcgataatc gctgctgcgc cttctgccgc tttgcggatg tgatccacaa
     9241 tcggctcacc agcaaccatg caataaatac cgttatcttt taatgggatg gaaccatcca
     9301 caaccaacac atactgccct ttgtacttct caagtgcgtt atgtttgttc tcttcgacct
     9361 gatgaccgaa ggcggcggaa agcacttcgt gatactccag agagatagtc tccagcacga
     9421 ggttttctac cgttggatgc gttgcacgaa gcagagattc cgtacaaccg gtgcactcct
     9481 gcgcgccaat ccagataact ggcggacgct gcgggttagt aaccgattcg gccatctctg
     9541 cagcggcttt gctacttaac cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat
     9601 cacgacggtt aatgccgtga gaatggatga gggtgttatc tccagtcatt tatagttatt
     9661 ccgttgcgaa gacctggcat atattttgcc tcaatcgcaa aatcaataat gcgatcgatg
     9721 cgccatttac cacacattta ttatggttat cgatatcatg atactgcggc ggggcgctaa
     9781 aacgaaggga agaagcatta gtgtaattaa ttaaagcaag ataatacgta tgtttgatca
     9841 attttcgagg cgataaccgg ccataaaaaa gcgcaacgta gaacaggaat tattctcacg
     9901 aaagaaatcc attaccatct ctttatccag tccatagcgg cgcgtcagaa tacccgcttc
     9961 ccaggcgcta agctggcgat ctccggtctt ttcgtacatc cggtgtgagt agcctaatac
    10021 aaaaccgcgt ttataatcgg cacagaattt tgtcacatta accgcactgt cggcatgcgt
    10081 tgcttttaat cccgccatta agcctttacc aaaatggtta ttcatcgttc acctcattcg
    10141 ctatatatcg ccatatatag cgatatttta ggcaaagaac gacttttgcg cacgctattt
    10201 tttcatgctt gtttaagacg taatccagta agagataaac ctatcaggag agatatttat
    10261 cgaaccattt cagtgtcctt tgccaggcaa gatcggcggc agatttgtca taacggggcg
    10321 tggaatcatt atggaatccg tgattaaccc ccggatagat atacgcctca taaaccttat
    10381 tattggcttt caacgccgcc tcgtaagcag gccagccctc gttgattcgg gtgtccagtt
    10441 ccgcgaagtg gagtagtaaa ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac
    10501 gaccataaaa cggcaccgcg caggccagtt ccggatacgc gacagccgcc gcgttcgata
    10561 cgccaccgcc atagcaaaat ccggtaatac ccactttgcc tgccgcttgc ggatagcgtt
    10621 gcataaactc aattgcggca aagaaatcat tcatcagttt ggttggatca acctgctgtt
    10681 gcagctcacg acctttatca tcatttcccg gataacctcc aacggaactt aagccgtcag
    10741 gtgccagggc gatatacccc gccttcgcca ctcgccgtgc cacatcttcg atatacggat
    10801 tcagtccacg attctcatgc accactacca cggctggcgt tttgccgctc atttttgcgg
    10861 gtttcaccag ataaccccgt acctcgccgt gaccatttgg cgaaggatac gtgatgtact
    10921 cagcaacaat ctccgggtcg gtaaactcta cctgagtcgc cagcgcataa tttggcttga
    10981 gcaaatcaaa caacgctaat gccgtcatcc cgcccaccgc atacttcgcc gcaaggttga
    11041 ggaactcacg tttcgagatt ttcccgtgag cgtaatagtc gtagtaatcc aacaactctt
    11101 gtgggaaatc tttggccgtt aaacgcggca tcgttgcact cctcaactgg tgttttttta
    11161 agcaaagcat aagcacgtgt ttttgcgcag tttttcgtca ctctgtgagc cagactacgg
    11221 gatacgcgct ggcgaatcgc taaactagaa acattgtttc gaaattgaac ggtggaaagg
    11281 agaggtcatg gtctggttag cgaatcccga acgttacggg cagatgcaat accgctattg
    11341 cggaaaaagc ggtttacgcc tgcccgcgtt atcgctcggt ttatggcaca atttcggtca
    11401 cgttaacgcg ctggaatcac agcgtgcgat cctgcgtaaa gcgtttgatt tgggcattac
    11461 gcactttgat ttagccaaca attacgggcc gcctccagga agcgcagaag agaactttgg
    11521 tcgcctgctg cgggaggatt ttgccgctta tcgcgatgaa ctgattatct ctaccaaggc
    11581 tggctacgat atgtggcccg gcccttacgg ctctggcggt tcacgtaaat acctgctcgc
    11641 cagcctcgac caaagcctga agcgtatggg gcttgagtat gtcgatatct tttactctca
    11701 tcgcgtcgat gaaaatacgc cgatggaaga aaccgcctct gcgctggctc atgcggtaca
    11761 aagcggtaag gcgctgtatg tcgggatctc ctcttactcg ccagagcgga cgcaaaaaat
    11821 ggtcgagttg ctgcacgagt ggaaaattcc gcttttaatt catcaacctt cgtacaattt
    11881 actgaaccgc tgggtggata aaagcggcct gctggatacc ctgcaaaata acggcgtggg
    11941 ctgtattgcc tttactcctc tggctcaggg attgctgacc ggaaaatatc tcaacggcat
    12001 tccgcaagat tcacggatgt atcgtgaagg gaataaagtt cgtggtctga caccgaaaat
    12061 gcttaccgaa gccaacctca acagcctgcg cttattgaat gaaatggcac agcagcgtgg
    12121 acaatcaatg gcgcaaatgg cgttaagctg gttgttgaaa gatgatcgcg tgacgtcggt
    12181 attgattggt gccagccgcg cggagcaact agaggagaac gtgcaggcgc tgaataatct
    12241 gacatttagc accgaggagc tggcgcagat tgatcagcat atcgccgatg gcgagctgaa
    12301 tctgtggcag gcgtcgtccg ataaatgacc tgttaataac gggcgcgcga accgcgcccg
    12361 aatcagatca gtgattatga cgagtcagtc ggtcaagata gcccatcaca aatgcagaaa
    12421 gcacaaacgt cagatggata atgacgtacc acatcagttt gttatccggg acatttttcg
    12481 catccataaa gacgcgcagt aggtgaatgg aagaaattgc cacaatcgac gctgctactt
    12541 tgtttttcag cgacgttgcg tccattttcc ccagccagtt cagcttctct ttgttctcgg
    12601 agatatccag ctgtgagacg aaattctcat aaccggaaaa catcaccatc accagcaaac
    12661 cgccaaccag cgtcatatcc accagcgaca gcaacacgag gatcaaatct gattccgcca
    12721 tcgagaagat attcggcagt acgtgaataa tctcctggaa gaacttcagc gccagggcaa
    12781 ctaacgccag cgaaaggcca aagtacacgg gggcaagcag ccagcgagaa gcatacattg
    12841 cattttcaag aaaacgttcc atacattcct gtcgtgtagt taaaacgggc aacagtatat
    12901 cgcaattgag taaacagcac gcaacagcaa atcaacatat acgttaaacc tttgtcgcat
    12961 aattctgagg tttagggatc ttcctggttc ccttatcaac gcatcgagag cgtctaccct
    13021 taaaggacgt tgaacctgaa gggagaaaaa cgatgtctca tctaaaagac ccgaccacgc
    13081 agtattacac tggtgaatat cccaaacaga aacaaccgac gccaggcatc caggcgaaga
    13141 tgacaccggt accggattgc ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc
    13201 gtaaagcact ggtgacaggg ggcgattccg gaataggtcg cgctgccgcc atcgcttacg
    13261 cgcgtgaagg ggctgacgtg gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg
    13321 atgtgaaaaa gatcattgaa gaatgcggtc gcaaagccgt tctgctgcca ggcgatttaa
    13381 gcgatgagaa attcgcccgt tcgctggttc acgaagcgca caaggcgtta ggcgggctgg
    13441 atattatggc gctggtcgcc gggaaacagg ttgccattcc tgatattgca gacctcacca
    13501 gcgaacagtt tcaaaagacc tttgccatta acgttttcgc gctgttctgg ctaacccagg
    13561 aagcgatccc cctgctaccg aaaggtgcaa gtatcatcac cacttcgtca atccaggcat
    13621 accagccaag tccgcattta ctggactatg cggctacgaa ggcggcgatt ctgaactaca
    13681 gccgtggcct ggcaaaacag gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag
    13741 gccctatctg gacggcactg caaatttctg gcggacaaac gcaggataag atcccgcagt
    13801 ttggtcagca aacgccgatg aaacgtgcgg ggcaaccggc ggaactggcc cctgtatatg
    13861 tttatctggc aagtcaggag tcgagctacg tcaccgcaga agtgcacggt gtgtgcggcg
    13921 gcgagcattt aggttaaaaa aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat
    13981 tcactcttac ctgatgtggc gaaaacgcct tatccgccta caaacgcatg aagattcaac
    14041 atgttgcaat agattttgta ggcctgataa gcgtagcgca tcaggcattg agcacctaat
    14101 gccggatgcg gcgtgaacgc cttatccggc ctacaaacgc acgaagattc aacatgttgc
    14161 aatagatttt gtaggcctga tgagcgtagc gcatcaggca attctacttt acttcgcttt
    14221 ggcggtttct tcgccgacca gacctatctt caggtaaccc gcctgatgca gcgtatccat
    14281 taccttcatc aacgtctcgt aatcgacggt tttatccgct cggaagaaga tggtggtgtc
    14341 tttcttgcct tcggttaacg cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc
    14401 gttaccgata aacatcgagt tgtctgcctt caccgacaga taaaccggtt tttccgggcg
    14461 cggctgcggc gtgctggtag aagcaggcaa gttcaccttc acatctaccg tcgctaacgg
    14521 tgccgccacc ataaagataa tcagcagaac caacatcacg tcgataaacg gcgtcacgtt
    14581 gatatcatgc atttcgccgt tatcgtcgag gttttcgtta agatgcattg ccatcggata
    14641 ttatcctgcg cgtaattttt gtgcgacacg aaccggatgc gcagcggcgc tggcttccag
    14701 atccaggtca cggctttgca gcaacaatac ctgcgctgca acatcaccca gcatcgcttt
    14761 aaagccgcca atctggcgtg caaatacgtt atagataacg accgcaggaa tcgctgccac
    14821 gaggccgatt gccgttgcta acagagcttc tgcgataccc ggcgcaacga ctgccaggtt
    14881 agtggtctgc gtttgcgcga taccaataaa gctgttcatg atgccccaga ccgtaccaaa
    14941 cagaccaacg aacggagaaa tcgcgccgat ggttgccaga tagccgttac cgcggcccat
    15001 ttgacgaccc actgcggcga cccgacgctc cagacggaag ctggtacgtt ctttaatacc
    15061 ttcgttatcg tcgctgcctt ctgacagttc cagctcattc tgcgcttcat tgagcaaatg
    15121 caggcttaag cttttgctac caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg
    15181 cgcttcagcc agcagttgct gctcgcgctt aaggcgacgc ttctgattga agaactctac
    15241 gctcttactg aagaagattg cccaggtgac tacggaggcc aaaataagcc caatcatcac
    15301 gcacttaacg acaatatcgg cgtgctgata cataccccag acggaaaggt ccgtctgcat
    15361 taaattatta cccactgtgt atctccagga cgcaagtcac aaaatctgcg cataataata
    15421 tcaaaacgac gtcgaattga tagtcgttct cattactatt tgcatactgc cgtacctttg
    15481 ctttcttttc cttgcgttta cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt
    15541 tctgctttat gccaattctt caggatgcgc ccgcgaatat tcatgctagt ttagacatcc
    15601 agacgtataa aaataggaat cccgacatgg cggacaaaaa gcttgatact caactggtga
    15661 atgcaggacg cagcaaaaaa tacactcttg gcgcggtaaa tagcgtgatt cagcgcgctt
    15721 cttcgctggt atttgagagt atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg
    15781 gcgagttgtt ctatggacgg cgcggaacgt taacccattt ttccttacaa caagcgatgt
    15841 gtgaactgga aggtggcgca ggctgcgcgc tatttccctg cggggcggca gcggttgcta
    15901 attcgattct tgcttttgtc gaacagggcg atcatgtgtt gatgaccaac accgcctatg
    15961 aaccgagtca ggatttctgt agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt
    16021 ttgatccgct gattggtgcc gatatcgtta agcatctgca gccaaacact aaaatcgtgt
    16081 ttctggaatc gccaggctcc atcaccatgg aagtccacga cgttccggcg attgttgccg
    16141 ccgtacgcag tgtggtgccg gatgccatca ttatgatcga caacacctgg gcagccggtg
    16201 tgctgtttaa ggcgctggat tttggcatcg atgtttctat tcaagccgcc accaaatatc
    16261 tggttgggca ttcagatgcg atgattggca ctgccgtgtg caatgcccgt tgctgggagc
    16321 agctacggga aaacgcctat ctgatgggcc agatggtcga tgccgatacc gcctatataa
    16381 ccagccgtgg cctgcgcaca ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga
    16441 aagtggctga atggctggca gaacatccgc aagtagcgcg agttaaccac cctgctctgc
    16501 ctggcagtaa aggacacgaa ttctggaaac gagactttac aggtagcagc gggctatttt
    16561 cctttgtgct taagaaaaaa ctcagtaatg aagagctggc gaactatctg gataacttca
    16621 gtttattcag catggcctac tcgtggggcg ggtatgaatc gttgatcctg gcgaatcaac
    16681 cagaacatat cgccgccatt cgcccacaag gcgagatcga ttttagcggg accttgattc
    16741 gcctgcatat tggtctggaa gatgtcgacg atctgattgc cgatctggac gccggttttg
    16801 cgcgaattgt ataacattgc cacttttgga caattttgca gacatttaat tgtgaaaagt
    16861 cttaaattgt tgcgtccggg atcaaggcgt cccggacgaa tcaggagtac aataggtaga
    16921 taaaggctta aacgctgttc cacaggaaag tccatggctg ttattcaaga tatcatcgct
    16981 gcgctctggc aacacgactt tgccgcgctg gcggatcctc atattgttag cgttgtttac
    17041 tttgtcatgt ttgccacgct gtttttagaa aacggcctgc tgcccgcctc atttttgcca
    17101 ggcgacagct tgttgatact ggcaggcgca ttgattgccc aaggggttat ggattttctg
    17161 cctacgattg cgattctgac cgccgcagca agtctgggct gctggctaag ttatattcag
    17221 gggcgctggt tagggaatac caaaacggtg aaaggctggc tggcacagct tcctgctaaa
    17281 tatcaccagc gcgccacctg catgtttgac cgccacggtc tgctggcgct gctggctgga
    17341 cgttttcttg catttgtccg tacgctgctg ccaaccatgg cgggaatttc cggtctgcca
    17401 aaccgccgct tccagttttt caactggtta agtggattgc tgtgggtcag cgtggtaacc
    17461 agttttggct atgccttaag tatgattccg ttcgttaaac gccatgaaga tcaggtaatg
    17521 acgttcctga tgatcctgcc aattgccttg ttaaccgctg gcttgttagg cacgctgttt
    17581 gtggtgatta aaaaaaaata ctgtaacgcc tgacgatttt ccccgttccc ggctgctgta
    17641 ccgggaacgt atttaattcc cctgcatcgc ccgcattctt gccgcatctt cccccggcgt
    17701 cacaccgaag taacgtttaa actcacggct aaattgcgat gcgctttcat agccgacgcg
    17761 catcgctgct gcgctggcct tcatgccgtc atggatgatc atcatccgcg ccttatgcag
    17821 acggtaattc ttcaaatact gcaacggcga ggtgctggtg acagacttaa aattatggtg
    17881 gaacgccgat acgctcatgt tggcttctgc cgccagttgc tcgacgctca ggttttcggt
    17941 gtatttattc tcaatccgtt tcagcacgcg gctaatcaga ctgaagtgag tctggcgact
    18001 gaccagcgcc agtaacgcgc cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg
    18061 gatgatctgt ttgccgagaa tacgcgcatc cagtggtcgc tccatcacgt cgagtaaccg
    18121 ctccgccgcg cataaaatct cttctgataa cgtggcggag ttaatcccgc tggctgccat
    18181 cgacggctgg aaatgctcat cttcgccaat gtccatcaac agttcctgta actgcaaaat
    18241 atcgacattg agacgcaacc ctgccagcgg cacctctgac gtggcatagg tttcgcactc
    18301 aaacggcaac ggcaccgtca gcagcaggta ttcattggca tcataacgaa acacgcgttc
    18361 attgatataa ccgattttat gcccggaaaa gagaattatg atgccaggct cgtacatcac
    18421 cggtgtacgt gcgaaaggcg tctcgccata caacaaacgc acatcgggca acagttctga
    18481 caaactattt tctttaattt tcagtttatt aactttatcc gccagcaagc ggcaaatctc
    18541 ttcacgtttc atatcgcgta atttcttagg aataatgcgg caatttgatt gtgcgcaatt
    18601 ttgtagcatt tctccagcac tctggaggaa taggcaagac attggcagaa atgagcattg
    18661 agagccaggg cgctggcgat cacaatgaaa aacatcaggc agatcgttct ctgccctcat
    18721 attggcccag caaagggagc aagtaatgaa caactttaat ctgcacaccc caacccgcat
    18781 tctgtttggt aaaggcgcaa tcgctggttt acgcgaacaa attcctcacg atgctcgcgt
    18841 attgattacc tacggtggcg gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga
    18901 tgccctgaaa ggcatggacg tgctggaatt tggcggtatt gagccaaacc cggcttatga
    18961 aacgctgatg aacgccgtga aactggttcg cgaacagaaa gtgactttcc tgctggcggt
    19021 tggcggcggt tctgtactgg acggcaccaa atttatcgcc gcagcggcta actatccgga
    19081 aaatatcgat ccgtggcaca ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc
    19141 gatgggctgt gtgctgacgc tgccagcaac cggttcagaa tccaacgcag gcgcggtgat
    19201 ctcccgtaaa accacaggcg acaagcaggc gttccattct gcccatgttc agccggtatt
    19261 tgccgtgctc gatccggttt atacctacac cctgccgccg cgtcaggtgg ctaacggcgt
    19321 agtggacgcc tttgtacaca ccgtggaaca gtatgttacc aaaccggttg atgccaaaat
    19381 tcaggaccgt ttcgcagaag gcattttgct gacgctgatc gaagatggtc cgaaagccct
    19441 gaaagagcca gaaaactacg atgtgcgcgc caacgtcatg tggggggcga cgcaggcgct
    19501 gaacggtttg attggcgctg gcgtaccgca ggactgggca acgcatatgc tgggccacga
    19561 actgactgcg atgcacggtc tggatcacgc gcaaacactg gctatcgtcc tgcctgcact
    19621 gtggaatgaa aaacgcgaga ccaagcgcgc taagctgctg caatatgctg aacgcgtctg
    19681 gaacatcact gaaggttccg atgatgagcg tattgacgcc gcgattgccg caacccgcaa
    19741 tttctttgag caattaggcg tgccgaccca cctctccgac tacggtctgg acggcagctc
    19801 catcccggct ttgctgaaaa aactggaaga gcacggcatg acccaactgg gcgaaaatca
    19861 tgacattacg ttggatgtca gccgccgtat atacgaagcc gcccgctaag ctttttacgc
    19921 ctcaaacttt cgttttcggg catttcgtcc agacttaagt tcacaacacc tcaccggagc
    19981 ctgctccggt gagttcatat aaaggaggaa cgtatggcta atccaaccgt tattaagcta
    20041 caggatggca atgtcatgcc ccagctggga ctgggcgtct ggcaagcaag taatgaggaa
    20101 gtaatcaccg ccattcaaaa agcgttagaa gtgggttatc gctcgattga taccgccgcg
    20161 gcctacaaga acgaagaagg tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa
    20221 gaactgttca tcaccactaa gctgtggaac gacgaccaca agcgcccccg cgaagccctg
    20281 ctcgacagcc tgaaaaaact ccagcttgat tatatcaacc tctacttaat gcactggccc
    20341 gttcccgcta tcgaccatta tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag
    20401 ggattaatca aaagcatcgg cgtgtgcaac ttccagatcc atcacctgca acgcctgatt
    20461 gatgaaactg gcgtgacgcc tgtgataaac cagatcgaac ttcatccgct gatgcaacaa
    20521 cgccagctac acgcctggaa cgcgacacac aaaatccaga ccgaatcctg gagcccatta
    20581 gcgcaaggag ggaaaggcgt tttcgatcag aaagtcattc gcgatctggc agataaatac
    20641 ggcaaaaccc cggcgcagat tgttatccgc tggcatctgg atagcggcct ggtggtgatc
    20701 ccgaaatcgg tcacaccttc acgtattgcc gaaaactttg atgtctggga tttccgtctc
    20761 gacaaagacg aactcggcga aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat
    20821 cctgaccagt tcggcggcta acatgcaaat tctcccggtg gcggtaatgt tccgctaccg
    20881 gacttttcag aaatcattta ttcccctcgc gtcccgcccg ttgttactct tccttgttca
    20941 ggaatgccaa atataaggac atcatcatgc agagccggaa gctcttaaaa gaacaactca
    21001 tctatatccg ggataaacgc aacggagagg tgaaaaacag atgaaaataa tacttctgtt
    21061 tttagcagcc ctggcaagtt ttaccgtaca cgcacagccc ccctcactga ccgtagaaca
    21121 aacagtccgg catatttatc agaactataa atcagatgcc actgcccctt attttggtga
    21181 aaccggagag cgggcgataa cttctgcgcg tattcaacag gcgcttaccc tgaacgacaa
    21241 tcttacgctg ccgggcaata ttggctggct ggattatgat ccggtttgtg attgtcagga
    21301 ttttggcgat ctggtgctag aaagcgttgc gataacccaa actgacgccg atcatgccga
    21361 tgccgttgtg cgctttcgta tctttaaaga tgataaagaa aagaccacgc agacactgaa
    21421 aatggtggcg gaaaatggtc gttgggtcat tgacgatatt gtcagcaatc atggcagcgt
    21481 cttacaagca gttaatagcg agaatgaaaa aacgctggcc gctttagctt cgttgcaaaa
    21541 agaacagccg gaagcctttg ttgccgaact ctttgaacat attgctgatt atagctggcc
    21601 gtggacgtgg gtggtttccg actcttaccg ccaggcggtt aatgccttct ataaaaccac
    21661 cttcaagacg gccaataatc ccgatgaaga tatgcaaata gaacggcaat ttatttacga
    21721 caatccgatc tgttttggcg aagagtcgct attttcacgc gttgatgaaa ttcgagtcct
    21781 ggagaaaacc gccgattccg cccgcattca tgttcgtttt acgctgacca atggcaacaa
    21841 cgaagagcaa gaactggttt tacagcggcg cgaaggcaag tgggaaatcg ctgattttat
    21901 ccgcccgaac agcggcagcc tacttaagca gattgaggca aaaaccgccg ccagattaaa
    21961 gcaatgagct gaattaaata acaattagcc ggaacaataa ataaaaggga acactatatg
    22021 aaaacgattt tcaccgtggg agctgttgtt ctggcaacct gcttgctcag tggctgcgtc
    22081 aatgagcaaa aggtcaatca gctggcgagc aatgtgcaaa cattaaatgc caaaatcgcc
    22141 cggcttgagc aggatatgaa agcactacgc ccacaaatct atgctgccaa atccgaagct
    22201 aacagagcca atacgcgtct tgatgctcag gactattttg attgcctgcg ctgcttgcgt
    22261 atgtacgcag aatgataaaa aaatccccgg cagcatgtca gttgccgggg atttttttta
    22321 acgtccaacc gccgctttag ggcgtttctt cgcaccagca ttcaccggac gagattgcgt
    22381 agacgacgct ttttttgccg tagcaggcgt ctgacgctgg gtcgccatcg gcgtatgttt
    22441 cgtcaacgcc ggacgggtat tgcggttctg gcgacgagct tcacgcatct cttcaatggt
    22501 tggcgcaggc actaagcaat cgcgacggct gccaatcaga tgctttttgc ccatcgcttc
    22561 cagcgcctgg cggattaacg gccagtttgc cggatcgtgg taacgcaaca acgctttatg
    22621 caaacgacgc tgcttgtcgc ccttcggtac gaagacgtct tcactcttat aaccaatctt
    22681 cgccagcggg tttttcccgg tgtaatacat ggtggttgag ttcgccagcg gcgatgggta
    22741 gaagttctgt acctggtcga gacggaaacg atgctttttc agccacagcg ccagattcac
    22801 catatcttca tcacgcgtac cggggtgcgc ggagatgaaa taggggatca gatactgctc
    22861 tttacctgcc tgttttgagt aagtatcgaa cagctcttta aagcggtcat agctgcccat
    22921 gcccggcttc atcatcttcg ataacggccc ttcttcggta tgttccgggg caatcttcag
    22981 ataaccgccg acgtgatggg tcgccaactc tttgatatag cgcggatctt ccacggcgat
    23041 gtcataacgt acgcctgagg cgatgaggat ttttttaatg cctttcagat cacgcgcacg
    23101 gcgatagagg ttgatcgtcg gttcgtggtt agtgtccatg tgcggacaaa tatccggata
    23161 aacgcacgac aaacggcgac aagtttgttc agcgcgtggc gatttgcagc gcaacatata
    23221 catgttggca gttggcccac cgagatcgga aatcacgccc gtaaaacctg gaacggtgtc
    23281 gcggatcgct tcgatctcat taatgatcga atcttccgaa cggctctgaa taatgcgccc
    23341 ttcgtgctcg gtaatagaac agaaagaaca gccgccaaag cagccgcgca taatgttgac
    23401 cgaaaaacgg atcatttcgt aagccggaat acgggcattg ccataggccg gatgtggcac
    23461 gcgcttgtac ggcagcgcaa aaacgctgtc catctcttcg gtagaaagcg ggatagcagg
    23521 cgggttgatc cacacatagc ggtcaccgtg tttttgcatc aatgcgcggg cgcagcctgg
    23581 gttggtttcg tggtgcagaa tgcgcgaagc atgggcgtac agcactttat cgcccttcac
    23641 tttctcgaaa gaaggcagca acacgtaggt tttttcccac ggtttcgggc gcggtggctg
    23701 cacggttacg gctttggctt cctgcttttt cggtgccacc ggtttgttat ccgcgcacgg
    23761 caaatcttca ccatacggat gcgggattgg gtcgattttt ccaggggtat caagacgggt
    23821 ggaatccacg ccgctccagc caggcagcgc ctctttcacg ataatcgcgg tattacgcac
    23881 atcgcggatt tcactaatcg gctcgcccat cgccagacgg tgcgccacct ccaccagcgg
    23941 acgctcaccg ttaccaaaca tcagcatgtc ggctttcgaa tccaccagca cggaacggcg
    24001 cacggtatcg gaccagtaat cataatgtgc ggtacggcgc agactagcct caataccgcc
    24061 gaggatcacc ggtacatctt tccacgcctc tttacaacgc tgggtataaa ccagtgtggc
    24121 gcgatccggg cgcttacccg cgacgttatc cggcgtgtag gcatcgtcat gacgtaaacg
    24181 gcgatcggcg gtataacggt tgatcatcga atccatgttg ccagcagtaa caccgaaaaa
    24241 cagattcggt ttacccagac gcatgaaatc gtctttgctg ctccagtctg gctgggcgat
    24301 gatcccgacg cgaaagccct gtgcttccag catacgaccg caaatcgcca tcccgaagct
    24361 tgggtgatcg acatacgcgt cgccagtaac caaaatgatg tcgcagctat cccagccaag
    24421 ttgatccatc tcttcacgag acatcggcaa aaacggtgcc ggtccaaaac aggcggccca
    24481 gtactgcggc caggagaaca ggtcgcgatc cggttggatc agggagatag agctcatttt
    24541 gcttccagaa atgataaaaa aataatcaaa ggccggggat tataagccgg aacgaaagag
    24601 aaatcgaaag gtattccata ctcgccctcc tcgggcgagt atgaagatta cggtaccgga
    24661 ttgaccaaaa gttgccctat cgagccacgg tccgccattt ccagcgtctg actgttgaag
    24721 tagaacggga agtgcgccca ggaaggctga ccgaaataaa caagcagctc cacttgtccg
    24781 tcaacccaaa cggtatcttt ccagcctctg tcttccggga acggcatcgc gccattcacg
    24841 ttacggatct ggaacattac gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc
    24901 gtccagcgtt cccacgttcc ttgctgcgcg gtgacatcaa tacggttgac gtcccacagt
    24961 tgtccattaa tacccgggtc atcacccaga ctgatatcgc gactgcgaat cggcgaaccg
    25021 gccatgattt cagttggcag caagcgcatc ggaagactgt ctgtgaccag cggcagaagg
    25081 ccggttgggc gcagcgttag caccagggta gaaaccagaa tgctggatgg ctcaaagaag
    25141 ccacgaatac gatcaacaat gctcgccgct tcgccacagg tgatcgacac ttcatcgccg
    25201 ttgctcatat ccaccagaat ctcgcggcgc tcgcccggtg ccagcgaaag ttgcttcacc
    25261 gatacaggag caggcaggaa tccctgatcg ccagaaatca catgtaacgg gcgaccatcg
    25321 ctcatctgta gttgataacg acgagagttc gacgcgttca gcaggcgcaa gcgcacccag
    25381 ccacgcgaga cttcaacgta cgggctttgt acaccgttaa ccagcagcgt atcaccaaca
    25441 aagccgccgc ttcccggttc gttgtattct ggcgtaccaa agttatccag ccgtttatcc
    25501 tgaataatga ccggaaaatc atccacacca taatggttgg ggataggcag cgacttgctg
    25561 acttcatctt ccaccagcca cattccggca aggccgttat agacctgctg agccgtgcgg
    25621 ttgggagtat tggcgtgata ccacagagta gctgcgttct ggcgaatagg cagtacgggt
    25681 gcccagtcag cgtttggcga catcatccgt gccggaccgc ccatcagcgg gcctggtacc
    25741 tgtagcccgg ctaccgtcat tgagacattt tctgtcaggc ggttgctgta aataagctta
    25801 acatcgtcgc ccttccagac gcggatagtc ggccccaggt aacgaccatt gattccccag
    25861 accgacgcgc gtgtccctgg cgtaaatgac cagtgcgcac gttgtacagt cataaacagc
    25921 ggttgcccac ggcgagattc aagcagcggc ggaacgggta gcggttgctg ttgcccggct
    25981 gcgctggcct tcaggggaac agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac
    26041 tgacgccgac tgagtgacat atttgctcca tgtaaaactg gctaaagtaa taccgtgcag
    26101 gaaagtgttc cccgcaaaaa tacagattgc cgacttaaac ttttccggcg gcttcgcgtt
    26161 ctgcgacttc tttatcgagc tcggcgattt tttgttccat tatcgaacga caatgggcag
    26221 ccagctcacg aacctgatct ttgccatact gactgacgtc aattggcggc agcatttcga
    26281 caatcaccag accgttgtgc agtcgattaa gattaatctt attcgaagtt gtagagacgc
    26341 acacgggaat aatcgggacg cccgccgcaa ttgccgcgtg aaatgctcca gtcttgaacg
    26401 gtagcaggcc gcgaccacgg ctgcgggttc cttccgggaa catccagatg gaaatacggc
    26461 gttttttgaa atgattcacg acttccgcaa tggtgccgtg agctttagtg cgattgtttc
    26521 tgtcgatcaa taagttgccg gttaaccagt acaactgccc gaagaagggg atccacagca
    26581 agctcttttt acctaccgtc accgtcggcg gttgcacaat gttcgatgct gtcaccatgt
    26641 catagttgtt ctggtggtta gcgatataga tagcattgcc gtagctttca gcatctgcag
    26701 gtttgcggca ctcaactttc aggccgaaca gcggcgcaag acgaccaaac atatgcccaa
    26761 aggtggccac atgtttcggg ttacgcgggc tgaaaaggca ataaatggag ccgaatacac
    26821 agactaagat gctgtaaatc acggtaataa taagacgaaa gatatatagc ataacaccct
    26881 ctgaaggtct gagagactgg cattgtacgt cacctgaaat caggttaggg cattgttaaa
    26941 agccgctctc tggaaaagcg tattgttaat cgcaacgcat gaataaacaa cggtttcaag
    27001 ggaattttta tcgtttccgg gggttgaata cgctgccgga tgacgactta acgtttcatc
    27061 cggcgttcct tgcaagcggg aggaaacagc gccctccccg gcatattact cttcgctatc
    27121 accgctgctg gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg
    27181 catcaacgta ccgcggcgtc cacgttcgcc agtgactttc tgtaactctt ccgggcgcag
    27241 tttaattttg cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacaa
    27301 ttgcgccaga ccatcttctc cacgcgcggc ttctgccgat ggaatgttga taatcttgtt
    27361 gcctttgccc ttcgacagct gcggcagatc acttaccggg aacatcaaca tacggcctgc
    27421 ctgagtgatt gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg
    27481 ggcattttcc ggtaaagtga tcaaagcctt acctgcacgg ttacgcgcca ccagatcgtt
    27541 aaaggtgcag acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct
    27601 ttccatcagc atatggtcaa cggtcgcccc aggcggcaac gttaatttgc cggtgagcgg
    27661 ctcgccctga ccacgcgccg acggcagcgt aatcgggtca atggcatagc tacgaccggt
    27721 ggaatcaaca aacactaccg gttggttgct cttacctttc accgccgctt tgaagctatc
    27781 acccgcttta taattcaggc ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca
    27841 gcccatctgc gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat
    27901 cgctttcgct tcttcgcgtt cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc
    27961 gtctgcctgc agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc
    28021 ctgcaactgg tcgcgctctt tttccagttc actctgctca ccgcgaatct tcatctcttc
    28081 cagtttggca agatgacgca gtttcagttc gaggatcgct tccgcctggg tttccgtaag
    28141 gccaaaccgc gacatcagcg ccggtttcgg ttcatcttca ttacgaatga tctcaatcac
    28201 ttcgtcgata ttgagaaacg ccaccagcaa accttcgagg atatgcaggc gcttgaggac
    28261 tttctccaga cgatagttca gtcggcggcg cacggtatcg cggcggaaca ccagccattc
    28321 ggagaggatt tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattaag
    28381 gttaatacga tagctctttt ccagatcggt ggtagcgaag aggtggttca tcacctgatc
    28441 catatccacg cggttggaac gcggcacaat caccaggcgg gtcgggttct cgtggtcaga
    28501 ttcatcgcgc agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg
    28561 ctccagtacg cgcgcacctg aaacctgatg cggcaatgcg ctgataacca ccgcgccatc
    28621 ttctttcttc cacaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg
    28681 gatctcggcg cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat
    28741 atccagcagc tgatcgagcg tggttttcgg ctggtcgatt aatgcgattg ccgcctgagc
    28801 cacttcacgc aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt
    28861 gccgttaagc aaaatgtttg gcagacgggc aggtagcatt ttcggctcct gcaaagtgcc
    28921 gtcgaagttt ggcacccagt cagccgtccc ctgccctagc tcgctcaata gcagctcgga
    28981 atatttcgac aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg
    29041 cgcgccccag ttcccctgac catcaaccag cggataacgg taagagaacg gttgcgccat
    29101 caggaccatc gcttcataac aggcgatatc gccgtgcgga tggtatttac ccagtacgtc
    29161 accgacggta cgggccgatt ttttaaattt ggcgctggca ttcaggccca gttcagacat
    29221 cgcatacaca atgcggcgct gaacaggttt cagaccatca ccaataaacg gcaacgcacg
    29281 gtccatgatc acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc
    29341 aaggcgctct gccatatcgc tcattaattc tgattcctca acttattcgc cagccttcaa
    29401 aggggcaata ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg
    29461 tgaatagcgc cgccggacgt gctccgacgg cgataaaggc atcaatgtgc cttgatatac
    29521 aactctttgc tgtagacata atcctgggga ttatgcagcg gaaaaccgcc aacgtagggt
    29581 ttaagcagtt tgattaacgg ctgatagtag ataggaatca gcggtgcctg ttggttgatg
    29641 atcacttctg cctgctgata caacgcatta cgctttgtcg catcagtgat ctgcgtggcc
    29701 tggtttagta aggcgtcata ctgcgcattt ttccagtgac cgacgttttc ttcactatcg
    29761 cttttgagcg tgttcaggaa gctggaagca tcattgtacg tcgcatccca cgactgccga
    29821 gacagcatga aatcaccggc tcgtcgggca tcaagatagg ttttccactc cattgtgcgc
    29881 agcgtcacct gtgcacccag ccattttttc cattcggaag acaacgctat cgcggtcttt
    29941 tcatgcagat cgtacttgtt gtagaacagc tcaaagcgta gcggatgaga ggcgtcgtat
    30001 cccgcctgtt tcagcaaggc ttttgccatc gcgacgcgct cactcattgg cttttgcagt
    30061 tcatcgaacg tcgtcgcgct aaagcctttt acctctggcg gcgtcagcgt ggttgcgggc
    30121 gttctcaacc ccagtacctt ttgcgcaata agctgtcgat caaccgtaag atatagcgcc
    30181 cgacgcactc gcacatcgtt aaatggcggt ttctcaaggt tgaagttgta atattcgctg
    30241 ttcagacgcg gaataattcg tagctcgcca ggcagtgatt tttcaatggc gggaatttgc
    30301 tgcgccggaa cccaggtgag atcgacctct cccgcgcgat agcggttata gccggtgacc
    30361 gaattatcta gcgccagata ctcaacctgt tgcaatactg tatgttgcgc atcgcggtac
    30421 tttggatttt tgcgtgcagt aatcttttcg ttaactaccc actgatcaag cacaaaggca
    30481 ccgttgtaaa ccatgttctc tggcttactc cagctatcgc catgtttagc gatgacatga
    30541 tgaggaaccg ggaacagcgt cggccaggcg agcatcgtcg tgaaccaagg aaccggctgc
    30601 tcaagcgtaa cttcaagagt acgatcatcc gtcgctttga cacccagcga tgtaacatct
    30661 gctttacccg caacaatagc tgcggcattg ttaatgtgcg cctgtgccag atagccagca
    30721 aaagggcttg ccgttttcgg gtcaaccgcg cgctgccagc cgacgacaaa atcctctgcc
    30781 gtcagaggct gaccgtcgga ccactgcaaa ccgctacgca gatggaaaat atagcgcttg
    30841 ccgccgtcca gtatctccca gcgttcagcc tgagcgggct gcacctggcc ttcaccgtcc
    30901 atccatacca gaccttcaaa cagatccagc acaatctgcg cggcagtatt ctcctccacc
    30961 ttttgcgggt cgagcgtacc tgggtcgcta tgattgttgt aacgaaagac ttgttgcggg
    31021 gcgagcggtg tgttggcggg aacgtcagca gcgtagagag gagccgcact taccaggctg
    31081 accagccata ataaatttcg cgtatacatg gtattcctta ccggagaaat tataagccca
    31141 acaacaatac gcctgtggca ttgttcgctc aaacttatcg cgagtgattt ggctcacact
    31201 ccggtaaaga aaaaagcgag ttgcccccgc ttttccatta attaacggat ttcattcaat
    31261 agttctggat gcttatccag aacacgtaaa agtttgattg tggaaggatg aggttgggca
    31321 ttgccttttt cgtaacgcga aaacgcattt acacctcccc caaaaatttc gcttgcctct
    31381 ttttgggtaa gagagagctt ttttcgaacc ttcactataa attcaggtgc cactgtttcg
    31441 gcattcaccg aagcccgaaa tgcctttact tgcgccatga aagcatctga ctcttcttta
    31501 ttcatgatgc tctcttcgca atggacacaa tataaaccgt ggataccttt caatactgtt
    31561 tttcgtccac ggaaggtgta tggaatatct ttaatgccag aaaccatttc tccctggtgg
    31621 caaaccggac atttcatatt acttctcctt aaacgagacg atcagtacgt catgaattac
    31681 cgtaatttta agataaacct ggcctgtaac aagcctgggt ctgtaaacat cctgccagat
    31741 agtatgatca gagtaggtgg tcatgctttt ataaaagtcg ctctctgata atccaatgat
    31801 aacattacac ataccatcaa aatccaaacc taactcatct gcatttaaca gtgcactacg
    31861 tgttgtacga acttgcccgg tattgacaag ttttttaacc tgactcaaac gtgtatgtgg
    31921 tgtgcgtttt tccataaccc ccgcctccct gttactttag ttataaccta aaaggttaat
    31981 tacagcaatg aaaaagcacc taaaaggtta gttagatgta cggagatagt gaccacacaa
    32041 aacgtattct ttaaggaaag tgattgacca tataagaaag tggcgcatta gtagcgccag
    32101 tttgaagcag gaatttataa gggaagctgg agtcaggcat cacgccaacg gcacataaac
    32161 atccgtttcc agtcgctgct ctgtcacccc ttcggcaaga ttggtgtagt gaaacagaat
    32221 cggtgcttta cgcattttct cgccgctagc aggcagccag tggcgaatga tgccccatac
    32281 cgtatgtgaa atatcgtcca gctcgccaac gtggcgggcc acggcataac gtccaccggt
    32341 aagttcacca ttactcacgc cataacgatt atcgggaatc ggttcgctaa cgctgccgca
    32401 gatatcaaag cgaaacgctt ccggtgcggt ggtggcaggg tcgtcccagg cgacgccaaa
    32461 agtctggctt tggttaacag gagaaagccc cgtctcttta cgccacatga taaatttcgc
    32521 cacgctatag ttgagcaact cagggctaca gcggtgcggc aacatcgcca cagggattga
    32581 tgggaaatcg ataatgttta catccagtgt caggtttgtc attttgcctc cctgcgaaat
    32641 tattcccggg aacgcgtccc gggagcggta acaattacgg atttactttg cggatctgtt
    32701 tgacgtcaat ttcaacagaa ttccagtctt tatcgacttc accctgaatc tcaaccgtat
    32761 ctttcggcgt caccgtcacg ccgttccagc gtttgtggtc gatatcaaca ttgatagtac
    32821 cgctggcatc tttgaacacg tagagatcgt cagagatgcg ttcaacgata ttgccgcgca
    32881 gggttaccca ggtgtcgtca cgcagggatt ttgcgctttc tacagtcgtt acgctgccgt
    32941 tcggcccctg gaatcctccg gcctgacttt gcgttgccga tgggccagaa aaaccgccct
    33001 gctctgctgc catcaccggt gcgctgcaca gggccattac tgcgattact gctgcgaatt
    33061 ttttcatgtt tattactccc tttaatgtct gtttccgagc atttaacaag atagtcctta
    33121 acaacttctt aagggaaaaa aataaaattt agtgctgtac agagcgcgtt acaacacggt
    33181 ttactggcag caaatacggt tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag
    33241 atgacatgct gattggcgac ggcatcaaaa cgggccttag taaaatgggt tttagcgtcg
    33301 actggtttac acaaggtcgt cagggaaaag aggcgcttta tagcgcacct tatgatgcgg
    33361 tgatcctgga tttaacctta ccaggcatgg atggtcgcga tattttgcgc gaatggcgag
    33421 aaaaaggtca gcgtgagccg gtactgatcc tgaccgcgcg cgatgcgctg gaggaacgtg
    33481 tagaagggct gcgtctggga gctgacgatt atctgtgtaa accttttgcg ttgatagaag
    33541 tcgccgccag gctggaagct ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc
    33601 gccacggtaa cgtcatgctc gaccccggca aacgtatcgc cacgctggct ggcgaaccct
    33661 taacactgaa accaaaagaa tttgccctgc tggaattact gatgcgtaac gctggtcggg
    33721 tactgccgcg caaactgatt gaagagaaac tgtatacctg ggacgaagag gtcaccagta
    33781 atgccgttga agtgcatgtg catcatctgc gacgcaaact cggtagtgat tttattcgta
    33841 ccgtgcatgg tattggctac acattaggtg agaaatgaaa tttacccaac gtcttagtct
    33901 gcgcgtcagg ctgacgctaa tctttttaat tctggcctcg gtgacctggc tgctttccag
    33961 ctttgtcgcc tggaaacaaa caacggataa cgtcgatgaa ttgttcgaca cccaactgat
    34021 gctgtttgcc aagcggttaa gtacgctcga tctcaacgaa atcaacgcgg cggatcgcat
    34081 ggcacagacg ccaaataaat taaaacacgg tcatgttgat gacgatgcgc tgacctttgc
    34141 catctttacc cacgacggca gaatggtcct taacgatggc gataacggag aagatattcc
    34201 ctatagctat caacgggaag gttttgctga cgggcaactg gtgggcgata aagatcaatg
    34261 gcgttttgtc tggatgacct cacctgatgg caaatatcgc atcgttgttg gtcaggaatg
    34321 ggaataccgt gaagacatgg cgctggcgat tgttgccggg caattgatcc cgtggctggt
    34381 cgcactgccg attatgttaa tcatcatgat ggtactattg ggtcgtgaac tcgcgccgct
    34441 gaacaaactg gcgctggcgc tgcgtatgcg tgaccctgac tcggaaaaac cactaaacgc
    34501 gactggcgta cccagcgaag tgcgtccgtt ggttgagtcg ctaaatcaac tgttcgcccg
    34561 cacacatgcg atgatggttc gtgaacgacg ctttacctcc gacgcagctc acgaacttcg
    34621 tagcccgtta acggcgctga aagtgcaaac cgaagttgcg cagctctctg acgatgatcc
    34681 gcaggcgcgg aaaaaagcgc tgcttcaatt acattacggg atcgatcgcg ctactcgtct
    34741 ggttgatcaa ctgctcactc tatcgcggct ggactcactg gataaccttc aggacgtcgc
    34801 ggagatcccg cctgaagatc tcctgcaatc gtcggtgatg gatatttacc acacggcgca
    34861 gcaggcgaaa attgacgtgc gactgacact caatgcccac ggcatcaaac gcaccggaca
    34921 accgctattg ctaagtttgt tggtgcgaaa tttgctggat aacgccgtgc gctacagtcc
    34981 acagggcagc gtggtagacg tcacgctgaa tgctgataat ttcatcgtga gggataacgg
    35041 ccccggtgtg acaccagagg cactggcgcg aattggcgaa cgcttctatc gcccacccgg
    35101 acaaaccgct accggcagcg ggcttgggct atcgattgtc cagcgaatcg ccaaattgca
    35161 tggcatgaat gttgaatttg ggaatgcgga acaaggtgga tttgaggcga aggtaagctg
    35221 gtaacttctc tgtctatgag agccgttaaa acgactctca tagattttac taatagcaaa
    35281 atataaaccg tccccaaaaa agccaccaac cacaaaccag acaaataccg agcgagaata
    35341 catataccca aaactcgaac atttcccgca taaagagttt tcttaagata agaataataa
    35401 gtggcgtaag aagaaaaaat gctgcaatca ttgcttgcaa tatacgatct tcgcctgaaa
    35461 cggaaggtat actgcaaata ttgcctcccg agtcaagcat ccagtcatac ttattgaaag
    35521 caaaaaaagc atctattaac caataagtaa acattatgta taataatgct tcaaatattg
    35581 atagtagttg tttttttaac atgtctgatt actcatt
//
LOCUS       DAFNBJ010000032        34897 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000032 DAFNBJ010000000
VERSION     DAFNBJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.032"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            134..601
                     /gene="slyB"
                     /locus_tag="L2X49_001529"
     CDS             134..601
                     /gene="slyB"
                     /locus_tag="L2X49_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBN4796635.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(648..1082)
                     /gene="slyA"
                     /locus_tag="L2X49_001530"
     CDS             complement(648..1082)
                     /gene="slyA"
                     /locus_tag="L2X49_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBN4796636.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            1283..1519
                     /locus_tag="L2X49_001531"
     CDS             1283..1519
                     /locus_tag="L2X49_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBN4796637.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            1522..2379
                     /locus_tag="L2X49_001532"
     CDS             1522..2379
                     /locus_tag="L2X49_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBN4796638.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            2379..4391
                     /locus_tag="L2X49_001533"
     CDS             2379..4391
                     /locus_tag="L2X49_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBN4796639.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(4392..4913)
                     /gene="sodC"
                     /locus_tag="L2X49_001534"
     CDS             complement(4392..4913)
                     /gene="sodC"
                     /locus_tag="L2X49_001534"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBN4796640.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(4994..5890)
                     /locus_tag="L2X49_001535"
     CDS             complement(4994..5890)
                     /locus_tag="L2X49_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBN4796641.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSTEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(5939..6178)
                     /locus_tag="L2X49_001536"
     CDS             complement(5939..6178)
                     /locus_tag="L2X49_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBN4796642.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            6281..6880
                     /gene="nemR"
                     /locus_tag="L2X49_001537"
     CDS             6281..6880
                     /gene="nemR"
                     /locus_tag="L2X49_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBN4796643.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            6917..7979
                     /gene="nemA"
                     /locus_tag="L2X49_001538"
                     /pseudo
     CDS             6917..7979
                     /gene="nemA"
                     /locus_tag="L2X49_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            8060..8467
                     /gene="gloA"
                     /locus_tag="L2X49_001539"
     CDS             8060..8467
                     /gene="gloA"
                     /locus_tag="L2X49_001539"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBN4796644.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            8570..9217
                     /gene="rnt"
                     /locus_tag="L2X49_001540"
     CDS             8570..9217
                     /gene="rnt"
                     /locus_tag="L2X49_001540"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBN4796645.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPTAEEV"
     gene            9310..13926
                     /locus_tag="L2X49_001541"
     CDS             9310..13926
                     /locus_tag="L2X49_001541"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBN4796646.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIEEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVICQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(13977..14324)
                     /gene="grxD"
                     /locus_tag="L2X49_001542"
     CDS             complement(13977..14324)
                     /gene="grxD"
                     /locus_tag="L2X49_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBN4796647.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            14658..15473
                     /gene="mepH"
                     /locus_tag="L2X49_001543"
     CDS             14658..15473
                     /gene="mepH"
                     /locus_tag="L2X49_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBN4796648.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            15601..16182
                     /gene="sodB"
                     /locus_tag="L2X49_001544"
     CDS             15601..16182
                     /gene="sodB"
                     /locus_tag="L2X49_001544"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBN4796649.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(16328..17497)
                     /locus_tag="L2X49_001545"
     CDS             complement(16328..17497)
                     /locus_tag="L2X49_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4796650.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(17663..17752)
                     /gene="ynhF"
                     /locus_tag="L2X49_001546"
     CDS             complement(17663..17752)
                     /gene="ynhF"
                     /locus_tag="L2X49_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBN4796651.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            18051..19076
                     /gene="purR"
                     /locus_tag="L2X49_001547"
     CDS             18051..19076
                     /gene="purR"
                     /locus_tag="L2X49_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBN4796652.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(19073..20005)
                     /locus_tag="L2X49_001548"
     CDS             complement(19073..20005)
                     /locus_tag="L2X49_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4796653.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            20118..21329
                     /locus_tag="L2X49_001549"
     CDS             20118..21329
                     /locus_tag="L2X49_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBN4796654.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            21620..22768
                     /gene="cfa"
                     /locus_tag="L2X49_001550"
     CDS             21620..22768
                     /gene="cfa"
                     /locus_tag="L2X49_001550"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBN4796655.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(22808..23449)
                     /locus_tag="L2X49_001551"
     CDS             complement(22808..23449)
                     /locus_tag="L2X49_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBN4796656.1"
                     /translation="MFTGIVQGTVKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            23664..25037
                     /gene="mdtK"
                     /locus_tag="L2X49_001552"
     CDS             23664..25037
                     /gene="mdtK"
                     /locus_tag="L2X49_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBN4796657.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(25078..26334)
                     /locus_tag="L2X49_001553"
     CDS             complement(25078..26334)
                     /locus_tag="L2X49_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBN4796658.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            26642..26718
                     /locus_tag="L2X49_001554"
     tRNA            26642..26718
                     /locus_tag="L2X49_001554"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26676..26678,aa:Val,seq:gac)
     gene            26723..26799
                     /locus_tag="L2X49_001555"
     tRNA            26723..26799
                     /locus_tag="L2X49_001555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26757..26759,aa:Val,seq:gac)
     gene            26907..27212
                     /locus_tag="L2X49_001556"
     CDS             26907..27212
                     /locus_tag="L2X49_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBN4796659.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            27338..28942
                     /locus_tag="L2X49_001557"
     CDS             27338..28942
                     /locus_tag="L2X49_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBN4796660.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLVVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(28954..29766)
                     /locus_tag="L2X49_001558"
     CDS             complement(28954..29766)
                     /locus_tag="L2X49_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796661.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDLGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLAQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(29770..30555)
                     /locus_tag="L2X49_001559"
     CDS             complement(29770..30555)
                     /locus_tag="L2X49_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBN4796662.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(30552..31220)
                     /locus_tag="L2X49_001560"
     CDS             complement(30552..31220)
                     /locus_tag="L2X49_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBN4796663.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(31284..31922)
                     /locus_tag="L2X49_001561"
     CDS             complement(31284..31922)
                     /locus_tag="L2X49_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBN4796664.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(31935..34037)
                     /locus_tag="L2X49_001562"
     CDS             complement(31935..34037)
                     /locus_tag="L2X49_001562"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBN4796665.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKELFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(34058..34684)
                     /locus_tag="L2X49_001563"
     CDS             complement(34058..34684)
                     /locus_tag="L2X49_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBN4796666.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8361 a         9136 c         8604 g         8796 t
ORIGIN      
        1 aattggttga tcctactttc attatgattt gctcatattt ggtagaacat gtaaccatgg
       61 attcacatat gccatatact ttgaccatga gggatgcttg cgtggcgttt catggtgaac
      121 aggagatttt tcaatgatta aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt
      181 cggttgtgtt aataacgaca ccctgtcagg ggatgtttat accgcttctg aagcgaaaca
      241 agtacagaat gtcagctatg gcaccatcgt taatgtacgt ccggtacaga ttcagggcgg
      301 tgatgattcc aacgttatcg gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa
      361 tactgttggt ggcggaaccg ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg
      421 cgtagctggt cagggcgtac agagtgcaat gaacaaaacg cagggtgtcg agctggaaat
      481 tcgtaaagac gatggtaata ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc
      541 tccgggccaa cgtgtcgtac tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta
      601 aataagtttg cgtgtggtca ggttactgac cacacgcccc cttcatttca ccctttggcc
      661 tgtaactcaa tgatattatg ctcaagtttt gcgatgagcg taatcagttg ttccagttcc
      721 tctgcggaga tgccatgtaa tatttccgcg cgggttttgt taataacagc ttccatttcg
      781 ctgatcagtg gctctgcctt ttccgtcagt ttaatacgtt tagcccgacg atcgctggca
      841 caagtttgac gcgaaattaa ccctttttct tccagttggt ccagagtacg gaccagtgat
      901 ggctgctcga tgccaatcgc ttttgccagt tgaatttgcg actggtctgg aggtaactga
      961 tggatattgt gtaacgtaac ccaatgggtt tgtgttaact ccagcggttt caggcgatgg
     1021 tctatcagag cacgccatat gcgcaccaac cgtgccagat cagaacctag tggcgattcc
     1081 aatttcatct ccttataatt agcttgctaa gatattatgc ggcttttaga atagtgtgca
     1141 gcaattgtat tgctaaaaca aatgtattgc tgcatttggt taccgtcaga catatttttc
     1201 agaaattgcg cgtaaatttt tcgcacttaa agaatattta ttaatctaac gcaatatatt
     1261 cggtcgtaaa ggaatctact ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca
     1321 agatctggtg ttcggtgcgt ccgtctactt tcctccgttt ttcaaagcct tcgcgtttgg
     1381 attcgtcatc tggcttgtcg tacaccgcct gcttcgtggc tggatctacg ccggtgacat
     1441 ctggcatccc ttgttaatgg atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc
     1501 aatactgatt gcgtggtaac tatgtcaatt aaaacaatta agtatttctc aacaatcatt
     1561 gtagcggtag ttgcggttct tgccggatgg tggctgtgga attattacat gcaatctccg
     1621 tggacacgcg acgggaaaat acgcgcagaa caggtttcta tcaccccaca ggtgtcggga
     1681 cgtattgttg agctgaatat aaaagacaac cagctggtta acgcagggga tcttctgctc
     1741 acgatcgata aaacgccctt tcagatcgcc gagctgaacg cacaggctca gctggcgaaa
     1801 gcgcaatctg acctcgcaaa agccaataac gaagcgaatc gtcgtcgtca tctctcacaa
     1861 aattttattt ctgccgaaga actcgatacc gctaacctca atgttaaagc gatgcaggcc
     1921 agtgttaatg ccgcacaggc cacgctgaaa cagacacaat ggcaactggc gcaaacggaa
     1981 attcgcgccc cggtgagtgg ttgggtgact aacctcacta cccgcatcgg cgattacgct
     2041 gacaccggga aaccgctgtt tgcccttgtc gacagccact cgttttacgt cattggttat
     2101 tttgaagaaa ccaagttgcg ccatatccgc gaaggtgcac cagcacaaat taccctctat
     2161 agtgataaca aaacgttaca gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag
     2221 agcgttgaaa gtgactcaag ccttattccg gatgttaaac ctaacgtccc ctgggttcga
     2281 ctcgcccaac gcgttcccgt ccgctttgcg ctggataaag tcccaggcga tgtcacgttg
     2341 gtgtcgggca ccacctgtag cattgccgta ggtcaataat gaacgcatcg tcatggtcct
     2401 tgcgcaattt gccctggttc agggccacgc tggcgcaatg gcgttatgcg ttacgcaata
     2461 ccattgccat gtgtctggcg ctgacggttg cctattattt aaatctggat gaaccctatt
     2521 gggcgatgac ctcggctgca gtggttagct ttcccaccgt tggcggtgtt atcagcaaaa
     2581 gcctcggacg catcgctggc agtttgctcg gagccattgc ggcactgctt cttgccgggc
     2641 atacgctcaa tgagccgtgg ttttttctat tgagcatgtc ggcgtggctt ggcttttgta
     2701 cctgggcctg tgcgcacttc acgaataacg tcgcgtatgc atttcaactg gcgggctaca
     2761 cggctgccat catcgccttt ccgatggtta atattactga ggccagccag ctgtgggata
     2821 tcgctcaggc gcgcgtttgc gaggtgattg tcggcatttt gtgcggcggc atgatgatga
     2881 tgatcctgcc tagcagttcc gatgctacag cccttttaac cgcattgaaa aacatgcacg
     2941 cccgactact tgaacatgcc agtttactct ggcagcctga aacaaccgat gccattcgtg
     3001 cagcacatga aggggtgatt gggcagatac tgaccatgaa tttgctgcgt atccaggctt
     3061 tctggagcca ctatcgtttt cgccagcaaa acgcgcgcct taatgcgctg ctccaccagc
     3121 aattacgtat gaccagtgtc atctctagcc tgcgacgtat gttgctcaac tggccctcac
     3181 cgccaggtgc cacacgagaa attctcgaac agttgctgac ggcgctcgcc agttcgcaaa
     3241 cagatgttta caccgtcgca cgtattatcg ccccgctacg cccgaccaac gtcgccgact
     3301 atcggcacgt cgccttctgg cagcgactac gttatttttg ccgcctttat ctgcaaagta
     3361 gtcaggaatt acatcgtctg caaagcggtg tagatgatca taccagactc ccacggacat
     3421 ccggcctggc tcgtcatacc gataacgccg aagctatgtg gagcgggctg cgtacatttt
     3481 gtacgttgat gatgattggc gcatggagta ttgcttcgca atgggatgcc ggtgccaatg
     3541 cattaacgct ggcagcaatt agctgcgtac tctactccgc cgtcgcagca ccgtttaagt
     3601 cgttgtcact tctgatgcgc acgctggtgt tactttcgct attcagcttt gtggtcaaat
     3661 ttggtctgat ggtccagatt agcgatctgt ggcaattttt actgtttctc tttccactgc
     3721 tggcgacaat gcagcttctt aaattgcaga tgccaaaatt tgccgcattg tgggggcaac
     3781 tgattgtttt tatgggttct tttatcgctg tcactaatcc cccggtgtat gattttgctg
     3841 attttcttaa cgataatctg gcaaaaatcg ttggcgtcgc gttggcgtgg ttagcgttcg
     3901 ccattctgcg tccaggatcg gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc
     3961 gggattttgt cgatcagcta agccgccatc caacactgag tgaaagcgaa tttgaatcgc
     4021 tcacttatca tcacgtcagt cagttgagta acagccagga tgcgctggct cgccgttggt
     4081 tattacgctg gggtgtagtg ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact
     4141 gggaatcgcg ttccgatccg ttatcgcgag tacgggataa ctgtatttca ctgttgcggg
     4201 gagtgatgag tgagcgtggc gttcagcaaa aatcactggc ggccacactt gaagaattac
     4261 agcggatttg cgacagcctt gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa
     4321 ttgtctggcg gctgtactgc tcgctttcgc aacttgagca agcaccaccg caaggtacgc
     4381 tggcctctta attacttaat tacaccacag gcatagcgtt caccgccacc gcccagcggt
     4441 ttaggttgat cggacatatt atcgccgcca acgtggacca tcagcgcttt gtctttgatt
     4501 tcatccagtg atttcagacg aggcgcgatg acggcatcgg tagctttgcc gtcattattg
     4561 acgaccagtg caggcagatc gcctaaatgc ccggcacctt ctggcccttc atgtttaccg
     4621 gtattttgtg gatcaagatg cccgcctgcg gattccgcgg cgctggcttt gccatctttg
     4681 gtggctggct ggcagcttcc tttggcatga atatggaagc catgttcacc ggggggtaat
     4741 gctttcagat cgggcgaaaa ctccagacct ttatcggttt cagtaatggt gacgctacca
     4801 attgactgcc ctaccccttg cgacgtgacg aggttcatct cgactttttc actggcagct
     4861 tgtgcgccgg ttgcaacaac cagcgccaga atagccagac taaaacgttt cataggacct
     4921 ccgttcattg tgcattcctg aataagtgta aaccagtgac acatttttga acggcggcta
     4981 ttcctaaaag tgcttacggt acgtcgtacc ccagtgccgc tttacggata cgaaaccatt
     5041 gttgacgggt cattttcagt gtttctgctt cgacagctgc ccgtacgcgc tcaattttac
     5101 ctgaaccgat aattggcagc ggctgcgatg gtaaacgtaa tacccaggcg taaaccacct
     5161 gttcaatcga gcccgcgttt aactcctctg ccaccacagc cagttcatca cgcagcggct
     5221 ggaaataatc atcattaaac agacgaccac caccaaggca ggaccacgcc atcggacgaa
     5281 cacgcagttg ttgtagttgg tcgagcgtgc catccagcag taacggctga tgcaccgggg
     5341 atatttccac ctgattagtg gcaagggtaa acggcagacg tgattgcaac agggcaaatt
     5401 gcgcaggcgt aaagttcgat acgccaaaat gacgcacttt gccgctctga tgcagatgtt
     5461 tgaacgcgtc cgccacttca tcggcatcca ttaacgggtc tggtcggtgg attaacagca
     5521 gatccagatg atcggtcgcg agattaatta gcgactgttc ggtgctctta atgatgtgat
     5581 cgcggtcagt gatgtaatga ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc
     5641 atttactgac gatttccatc cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa
     5701 acgccgcttc gcactgatag ccaccataaa tatcagcatg gtccacggtg gtcacgccga
     5761 gatccagatg ctcttcaata aaactgacca gctggcgggc ggacatattc cagtccatca
     5821 atcgccagta gcccatcaca aaacgggaaa actccgggcc ttgcggcgca atagtaatac
     5881 gctgaaccat aatcgcttcc tcttatcaga tatgagagga gtatacgcaa gattaggttc
     5941 aaaagagtga tggttgctcc ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc
     6001 gcattaatcg ctgccgacaa aggcgcagca cctcttgttt ttcgccatcg ctcattttat
     6061 tccagttaaa acgctcatca cgactacgaa aacagccgcg acaaaacccg cgttcgtcag
     6121 actggcaaat tccccggcac gggctctgga cgggaaagaa ctctaattgc tccgccactt
     6181 cgccctcctc agataagatt attaccatta ttgaagctgt taatgtccaa agtagcaact
     6241 ttgcttgcac tagaccgact ggtctactac actccaacgc atgaacaaac acaccgaaca
     6301 tgatactcgc gaacatctcc tggcgacggg cgagcaactt tgcctgcaac gtggattcac
     6361 cgggatgggg ctaagcgaat tactaaaaac cgctgaagtg ccgaaagggt ccttctatca
     6421 ctactttcgc tctaaagaag cgtttggcgt tgccatgctt gagcgtcatt acgccgcata
     6481 tcaccagcga ctgactgagt tgctgcaatc cggcgaaggt aactaccgcg accgcatact
     6541 ggcttattac cagcaaacac tgaaccagtt ttgccaacat ggaaccatca gtggttgcct
     6601 gacagtaaaa ctctctgccg aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga
     6661 taaaggtgct cgcggcgtga tcgccctgct ctctcaggcg ctggaaaatg gccgtgagaa
     6721 ccattgttta accttttgtg gcgaaccgct gcaacaggca caagtgcttt acgcactgtg
     6781 gcttggcgcg aatctgcagg ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct
     6841 ggcccatgta aaaaacatta ttgcgacgcc tgccgtttag caggcatttt ttatcaccag
     6901 acgaccggga gcctttatgt catctgaaaa actgtattcc ccactgaaag tgggcgcgat
     6961 cacggcggca aaccgtataa ccgggtgaca ttcctacccc gttgatggcg gaatactatc
     7021 gccaacgtgc cagtgccggt ttgattatta gtgaagccac gcaaatttct gcccaggcaa
     7081 aaggatatgc aggtgcgcct ggcatccata gtccggagca aattgccgca tggaaaaaaa
     7141 tcaccgctgg cgttcatgct gaaaatggtc atatggccgt gcagctgtgg cacaccggac
     7201 gcatttctca cgccagcctg caacctggcg gtcaggcacc ggtagcgcct tcagcactta
     7261 gcgcgggaac acgtacttct ctgcgcgatg aaaatggtca ggcgatccgt gttgaaacat
     7321 ccatgccgcg tgcgcttgaa ctggaagaga ttccaggtat cgtcaatgat ttccgtcagg
     7381 ccattgctaa cgcgcgtgaa gccggttttg atctggtaga gctccactct gctcacggtt
     7441 atttgctgca tcagttcctt tctccttctt caaaccatcg taccgatcag tacggcggca
     7501 gcgtggaaaa tcgcgcacgt ctggtactgg aagtggtcga tgccgggatt gaagaatggg
     7561 gtgccgatcg cattggcatt cgcatttcgc caatcggtac tttccagaac acggataacg
     7621 gcccgaatga agaagccgat gcactgtatc tgattgaaca actgggtaaa cgcggcattg
     7681 cttatctgca tatgtcagaa ccagattggg cggggggtga accgtatact gatgcgttcc
     7741 gcgaaaaagt acgcgcccgt ttccacggtc cgattatcgg cgcaggtgca tacacagtag
     7801 aaaaagctga aacgctgatc ggcaaagggt taattgatgc ggtggcattt ggtcgtgact
     7861 ggattgcgaa cccggatctg gtcgcccgct tgcagcgcaa agctgagctt aacccacagc
     7921 gtgccgaaag tttctacggt ggcggcgcgg aaggctatac cgattacccg acgttgtaat
     7981 ccaacattgc gagcggcgta aagccgccgc tatactaaaa caacattttg aatctgttag
     8041 ccattttgag gataaaaaga tgcgtcttct tcataccatg ctgcgcgttg gcgatttgca
     8101 acgctccatc gatttttata ccaaagtgct gggcatgaaa ctgctgcgta ccagcgaaaa
     8161 cccggaatac aaatactcac tggcgtttgt tggctacggc ccggaaaccg aagaagcggt
     8221 gattgaactg acctacaact ggggcgtgga taaatacgaa ctcggcactg cttatggtca
     8281 catcgcgctt agcgtagata acgccgctga agcgtgcgaa aaaatccgtc aaaacggggg
     8341 taacgtgacc cgtgaagcgg gtccggtaaa aggcggtact acggttatcg cgtttgtgga
     8401 agatccggac ggttacaaaa ttgagttaat cgaagaaaaa gacgccggtc gcggtctggg
     8461 caactaatct cctgccgggc gtgaactcat cgcgcccgca tctttactgc atcgacaagt
     8521 aatatttgtc ataatgcgcg ctgcaattta tccgtattaa gagaatcaga tgtccgataa
     8581 cgctcaactt accggtctgt gcgaccgttt tcgtggtttt tatcctgttg tgatcgatgt
     8641 tgaaacagcc ggatttaacg ccaaaaccga tgcgctgctt gagattgccg ccatcaccct
     8701 gaaaatggat gaacaaggct ggctgatgcc ggacaccaca ttacatttcc acgtcgaacc
     8761 atttgtcggc gcaaatttgc aaccagaagc cctcgccttc aacggcattg acccgaacga
     8821 tcccgatcgc ggcgcggtca gcgaatacga ggcgctgcac gaaattttta aagttgtacg
     8881 taaaggtatt aaagcgagcg gctgtaaccg cgccattatg gtggcgcaca acgccaattt
     8941 tgatcacagc tttatgatgg ccgccgcaga acgcgcctcg ctgaaacgta acccgttcca
     9001 ccctttcgcc acttttgaca ctgctgcact ggccgggctg gcactcggac aaaccgtatt
     9061 gtcaaaggct tgccagaccg ctggcatgga cttcgacagc acccaggcgc actccgcgct
     9121 gtacgacacc gaacgcactg ctgtgctgtt ttgtgaaatc gtcaaccgct ggaaacgtct
     9181 gggaggctgg ccgctaccca ccgccgaaga ggtgtaatcg agtcgatgcc tgatgacata
     9241 taatgattca ggcatctata gtgaggctat tccacgcatc ctgcatgata ttcacgggga
     9301 atagcgttaa tggcagataa tccagaccct tcatcgctcc tgccggacgt gttttcaccg
     9361 gcgacccgcg actggtttct tcgcgccttt aaacagccga ccgctgtcca gtcgcaaacc
     9421 tggcatgtgg cggcgcgagg cgaacatgcg ctggtgattg caccgactgg ctccgggaaa
     9481 acgctggcag catttctcta cgccctcgat cggctcttcc gcgaaggcgg cgaagatacc
     9541 cgcgaggcgc ataagcgtaa aacctcacgc atcctctata tttcaccgat aaaagccctg
     9601 ggcaccgacg ttcagcgcaa cttgcagatc ccgttgaagg gtattgccga tgaacggcgg
     9661 cggcgcggcg aaacggaagt caatcttcgc gtagggatcc gtactggcga tacgcctgca
     9721 caggaacgca gcaaactcac ccgtaatccg ccggatattc tgatcaccac acccgaatca
     9781 ctctatctga tgctgacctc ccgcgcgcgc gaaacgctac gcggcgtcga aacggtaatt
     9841 attgatgaag tccacgcggt agcgggcagt aaacgtggtg cgcatctggc gttaagtctg
     9901 gagcggctcg atgcgctgct ccacacctca gcacagcgaa ttggcctttc tgccactgtg
     9961 cgctcagcca gcgatgtggc agcatttctt ggtggcgatc gcccggttac ggtagtcaac
    10021 ccgcccgcaa tgcgtcatcc gcagatacgg attgtcgtac cggtcgccaa tatggatgat
    10081 gtctcatcgg tcgccagcgg cactggcgaa gacagccatg ccggccggga aggctccatc
    10141 tggccatata ttgaaacggg catccttgat gaagtgttgc gccatcgctc gaccattgtc
    10201 tttactaatt cgcgggggct ggcggaaaaa ctgacggcac gattaaatga gctttacgcc
    10261 gcacgcttac agcgttcccc gtctatcgcc gttgatgcgg cccatttcga gtcgacctcc
    10321 ggcgcaacct ctaaccgtgt acaaagtagc gacgttttta ttgcccgctc acaccacggc
    10381 tccgtctcta aagaacaacg agcaatcacc gaacaggcgc tgaaatcggg tgaattacgc
    10441 tgcgtggtcg caacctccag tcttgaactg gggattgata tgggcgcggt ggatctggtg
    10501 attcaggtgg caacgccgct ttctgttgcc agtgggttac aacgcattgg tcgcgccgga
    10561 catcaggttg gcggtgtatc taaagggctg tttttccccc gtacccggcg tgatttagtc
    10621 gattccgcag tcattgtaga gtgtatgttc gcaggcaggc tggaaaacct gacaccaccg
    10681 cataatcctc tcgacgtcct tgcgcagcaa accgttgccg ccgcggcgat ggatgcatta
    10741 caggtagacg aatggtactc ccgcgtacgc cgtgccgcac cgtggaaaga tctgccaaga
    10801 cgtgtttttg acgccacgct ggatatgctt tccgggcgct atccctctgg cgatttttct
    10861 gcttttcgcc ccaaactggt ctggaacagg gagaccggga tattgaccgc ccgacctggc
    10921 gctcaattgt tggcggttac cagcggcggc accattccgg atcgtggcat gtatagcgtg
    10981 ttattacccg aaggtgaaga aaaggccggt tcgcggcggg tgggtgaact ggatgaggag
    11041 atggtatatg agtcgcgggt gaacgacatt atcactctcg gcgctacctc atggcggatc
    11101 cagcaaatca cccgcgatca ggtgattgtg actcctgctc cgggtcgttc tgcccggctc
    11161 cccttctggc gtggtgaagg taacggacgt ccggctgaat taggcgagat gatcggcgat
    11221 tttcttcatt tgctggcgga tggcgcgttc ttttccggga ctattccccc gtggctggca
    11281 gaagaaaata cgatcgccaa tattcagggg ttgattgagg agcagcgcaa cgcgacgggc
    11341 atcgttccgg ggagtcgcca tctggtcctc gaacggtgcc gtgatgaaat tggtgactgg
    11401 cgtattattt tgcactctcc ctatggaaga cgggtgcatg aaccctgggc gctggcgatt
    11461 gccgggcgaa tacatgccct atggggcgct gacgcgtcgg tggtcgccag tgatgacggc
    11521 attgttgcac gtattcctga caccgatggt aaattgcccg atgccgcgat ttttttgttt
    11581 gaaccagaaa agttgctgca aattgtccgc gaggcggtag gcagttcggc acttttcgcc
    11641 gcccgttttc gcgaatgcgc cgcgcgggca ttattaatgc cgggccgcac gccgggccat
    11701 cgcaccccgc tttggcaaca acggctgcgc gccagtcagt tgctggaaat cgctcaggga
    11761 tatccggatt ttccggtcat tctcgaaacc ctacgcgaat gtctgcaaga tgtttatgat
    11821 cttcccgcac tggaacgttt gatgcgtcgc ctgaacggtg gcgaaattca aatatccgat
    11881 gtaacgacta ctacgccctc gcctttcgcc accagtttat tgttcggcta tgtcgcggaa
    11941 tttatgtacc agagcgacgc cccgctggca gagcgccggg catccgtact gtcgctggac
    12001 agcgagttac tgcgcaatct actcggacag gtcgatccgg gggaattact cgacccgcag
    12061 gtcatttgcc aggtggaaga agagttgcaa cgactggctc ctggcagaag agcgaaaggt
    12121 gaagaaggat tgttcgacct gctgcgcgaa ctggggccaa tgaccgttga agacctggcg
    12181 caacggcata caggcagcag tgaagaggtt gcgtcgtatc tggaaaatct tcttgcagta
    12241 aaacgaatct tcccagcgat gattagcgga caggagcgtc ttgcctgtat ggatgatgcc
    12301 gccaggctgc gtgatgccct cggcgtacga ctaccagagt cattgccaga gatttattta
    12361 catagagtca gttacccgct tcgcgacctc tttctgcgct atctccgggc tcatgctctg
    12421 gtcacggctg aacaactggc tcatgagttt agtctcggta ttgccattgt cgaagagcag
    12481 cttcagcaac tgcgtgaaca gggtctggtg atgaatctgc aacaagacat ctgggtgagc
    12541 gatgaagtat ttcgtcgtct gcgtttgcgc tcgctgcaag ccgccagaga agcgacgcgt
    12601 cccgttgcag ccacgaccta tgcgcgattg ctgctggaac gtcagggcgt attacccgcc
    12661 accgatggta gcccggcgct ctttgcctca acatcgccag gcgtttatga gggcgtagat
    12721 ggcgtgatgc gggtgatcga acagcttgcc ggagtcggtt tacccgcctc actctgggaa
    12781 agccagatcc tgcctgcccg cgtacgcgac tattcgtcag aaatgctcga tgaattactg
    12841 gcaaccggtg cggttatctg gtcggggcaa aaaaaactgg gtgaagatga cggcctggtg
    12901 gcactgcatc tacaggaata tgctgcagaa tcgttcactc ccgccgaagc ggatcaggcg
    12961 aatcgttcgg cgctgcagca agcgatagtc gctgttctgg ctgacggagg agcctggttt
    13021 gcacaacaaa tcagccaacg gatacgcgac aaaatcggcg aatcggttga tctctctgcc
    13081 ctgcaagagg cgttatgggc gctggtctgg caaggcgtca tcaccagcga catttgggca
    13141 ccgttacgcg ccctcacccg cagcagttcc aacgcacgca cctcaactcg ccgcagtcac
    13201 cgggctcgtc gtggacgtcc tgtctatgcg caacccgtct cgccgcgggt atcttacaac
    13261 acaccaaatc tggctggacg ctggtcgtta ttgcaggtgg agccactaaa cgataccgaa
    13321 aggatgcttg cgctggcgga aaatatgctc gaccgctacg gcatcatcag tcgtcaggca
    13381 gtgatagccg aaaatatccc tggcgggttt ccatcgatgc aaacgctttg tcgaagtatg
    13441 gaagactccg ggcgaattat gcgaggtcgt tttgtagaag gtctgggtgg cgcgcaattc
    13501 gctgaacgtc tgactattga ccgattgcgc gatctggcga cacaagccac gcaaacgcgc
    13561 cactatacac cagtggcgct ctctgccaac gatccggcta atgtgtgggg aaatcttctg
    13621 ccctggcctg cacatccggc aacgctggtt ccaacgcgtc gggcgggtgc gctggtggtc
    13681 gtttctggcg gcaaattgtt actctatctg gcgcaaggtg gcaaaaaaat gctggtctgg
    13741 caggaaaaag aggaattact cgccccagag gttttccacg cgctgactac cgcactgcgt
    13801 cgcgaaccac ggctgcgctt tacgctaaca gaagtgaatg atctaccggt ccggcaaacg
    13861 ccgatgttta cgctgctgcg tgaggcggga ttttcaagtt cgccacaagg gctggattgg
    13921 ggatagagaa aggactgacg gatgcccgtt cgcatccgtc agtattgcag gacggattat
    13981 tccgcgtccg gctcttcaga cttgtattta gcggcagttt ctttgatcag ctgctgcagt
    14041 tcgccacgct gatacatttc gatcacgata tcacaaccgc cgaccagctc gccgtcaacc
    14101 cacagttgcg ggaaggtcgg ccagttagca tatttcggca gttccgcacg aatgtccgga
    14161 ttctgcagaa tatcaacata ggcaaaacgt tcgccacatg cggcaagcgc ctggactgcc
    14221 tgggcagaga aaccgcagct cggcagtttc ggtgaacctt tcatgtacag caggatcggg
    14281 ttttcagcaa tctggcgttg gattttttcg atagtggtgc tcattgtctt gcttcctcaa
    14341 acttctttac ggcagtaatc tgacattgta gcgggtcagt gcggcatcgg aaaataacat
    14401 tttcatcacg cttttgctat tttatccctt tgctctattt gttgcatttc aaatattcgt
    14461 ttcttctctg atgccggcaa actggcgtat ttataaccat ttttagcttt cactgctgca
    14521 tttttttgcg ctcgccaacg aaacgtattt tttaacaata aaagctatta actttctctt
    14581 cttctatgca ttagaatcat caagttttgt aaatcagacg caggcatgat agacctgcct
    14641 taacagaggg acgctcagtg gcgcggataa accgtatttc gatcacgctc tgtgctttac
    14701 tttttaccac cctgccttta acgcctatgg cccatgcttc aaagcaagcc agggagagtt
    14761 ctgctaccac tcatatcacc aaaaaagcag ataaaaagaa aagcacggca accaccaaaa
    14821 agacccagaa aacagcgaaa aaagccgcca gtaaaagtac gaccaaaagc aaaaccgctt
    14881 cttccgttaa aaaatcttcc attaccgctt ctaaaaacgc caaaactcgc agcaaacacg
    14941 ccgtcaataa aacggcctca gccagcttta ccgaaaagtg taccaaacgt aagggctata
    15001 aatcgcattg tgtgaaagtc aaaaatgccg cgtcaggaac tcttgccgac gcgcacaaag
    15061 cgaaggtgca aaaagctacg aaagtggcaa tgaataaact gatgcagcaa attggtaagc
    15121 catatcgttg gggtggcagc tcaccgcgta ccggttttga ttgcagcggc ctggtttatt
    15181 acgcttataa agatttggtg aaaattcgta ttccgcgtac ggcgaatgaa atgtatcacc
    15241 tgcgtgatgc agcgccaatc gaacgtagtg aactgaaaaa cggcgacctg gtctttttcc
    15301 gtactcaggg acgcggcaca gccgatcatg tcggcgtgta tgtcggcaac ggcaaattta
    15361 ttcagtcacc gcgtacaggt caggaaattc aaatcacttc tctcagtgaa gactactggc
    15421 agcgccacta tgttggcgct cgtcgggtaa tgaccccaaa aacacttcgc taaaacttta
    15481 ccctgttgtt acggcaacag ggtaagttca tcttttgtct caccttttaa tttgctaccc
    15541 tatccatacg cacaataagg ctattgtacg tatgcaaatt aataataaag gagagtagca
    15601 atgtcattcg aattacctgc actaccatat gctaaagatg ctctggcacc gcacatttct
    15661 gcggaaacta tcgagtatca ctacggcaag catcatcaga cttatgtcac taacctgaac
    15721 aacctgatta aaggtaccgc gtttgaaggt aaatcactgg aagagattat tcgcagctct
    15781 gaaggtggcg tattcaacaa cgcagctcag gtctggaacc atactttcta ctggaactgc
    15841 ctggcaccga acgccggtgg cgaaccgact ggaaaagtcg ctgaagctat cgccgcatct
    15901 tttggcagct ttgccgattt caaagcgcag tttactgatg cagcgatcaa aaactttggt
    15961 tctggctgga cctggctggt gaaaaacagc gatggcaaac tggctatcgt ttcaacctct
    16021 aacgcgggta ctccgctgac caccgatgcg actccgctgc tgaccgttga tgtctgggaa
    16081 cacgcttatt acatcgacta tcgcaatgca cgtcctggct atctggagca cttctgggcg
    16141 ctggtgaact gggaattcgt agcgaaaaat ctcgctgcat aataactgat ggcaaatgca
    16201 gcattgcctg atgcgcttcg cttaccatgc caacgcggat gaggcgttaa cagcgcattc
    16261 ggcaagataa atcgcacgtt gtcagcaact gtaacgcaga aggttatcct tctgcgtttt
    16321 tgtttaatta gctgttagca acgcaaactg tttcaggttg ttttctggct gacataaaca
    16381 ccagcaataa tgccagtccc gcgacaatcg ctcccatcac cggcacaaag ctgtatccca
    16441 gcccagcgga aattaccgca ccaccagcag ctgctcccag cgcatttcca agattaaagg
    16501 caccaatatt gactgatgaa gacagacctg gcgcttcact ggcgacacgc atcacgcgca
    16561 tctgtaacgg cggtacgacc gcaaaggttg ctgcgcccca caccaccatg ctaatagctg
    16621 cgccgaactc attgcgggcc aggaacggga ttgccagcat aatcaccatc aacaacaaca
    16681 aaaagccttt caacgtgccg ttaactgaac gatctgccag tttgccgccg agatagttac
    16741 cgatagagaa tccgacacca atcagcacca gcattgccgt gacgaacacc ggtgttgcgt
    16801 gggtaatact ttgcagtacc ggagagatat aggtgtagag agtaaacatt gcaccagctc
    16861 ccagtaccgt cgtcagcaat gcagacagca cctgcggacg cattaatacc gccagctctt
    16921 ttttcacttc aggtcgtgcc cctgcaccac ctttaggtaa tgagaagaac agacttacca
    16981 ttgaaatcac tcccagcccc gccgttgcca gaaatgacat ccgccagccg atggtttcac
    17041 ccaaccaggt cgccgccggc acgccaccga tatttgccag ggttaacccc ataaacatag
    17101 tggcaactgc gctggcctgt ttatgttttg gcaccacgct tgcggccacg actgaaccca
    17161 aaccaaaaaa tgctccgtga ttcaggctgg tcaaaatgcg tgaaagcatc agggtcatat
    17221 aatccggcgc gatggcggaa agtacgttgc cgagcgtgaa aattgccatc aggaaaatca
    17281 acgcactgcg gcgggcacga tgagaaagta gaagcgtcat cagcggcgcg ccaaccatta
    17341 cgccaactgc ataggcactg attaacattc cggcagcggg aatcgagaca tccacaccgc
    17401 gcgcaatgac gggcaacaaa cccattggcg agaactccgt tgtcccgata ccaaacgcgc
    17461 caatcgccag cgccagcaac ggatagttaa ttttcatgcc ttatctccac ctcttcgcgt
    17521 cattacgcga tattcattaa agtggcgaaa gcatgacagc aatcacaaaa aaatgaaaat
    17581 aacaaaaaga gaaaacactt ttgccatttt gctaacaaac aggaaggaga tgcgagggag
    17641 aacgcgctcc ctcgagagga aatcagtgca gcgcggcagt caaacccacg gccacgatca
    17701 aaccgaggac gataatcgtt gttaccagtg aaaatttaag gtcggtgctc atcaagtttt
    17761 ctcctttttt attaccacac aaatagtgat attacgcatt tttacacact gtgatgaaaa
    17821 aatctcccgt catttataat gataagtgtt tttaccactt ccccttttcg tcaagatcgg
    17881 ccaaaattcc acgcttacac tatttgcgta ctggccattg accccttcct gacgctccgt
    17941 gtcgtttttc cggcgtaccg caacactttt gttgtgcgta aggtgtgtaa aggcaaacgt
    18001 ttaccttgcg attttgcagg agctgaagtt agggtctgga gtgaaatgga atggcaacaa
    18061 taaaagatgt agcgaaacga gcaaacgttt ccactacaac tgtgtcacac gtaatcaaca
    18121 aaacacgttt cgtcgctgaa gaaacgcgca acgccgtgtg ggcagcgatt aaagaattac
    18181 actactcccc tagcgcggtg gcgcgtagcc tgaaggttaa ccacaccaag tctatcggtt
    18241 tgctggcgac cagcagcgaa gcggcctatt ttgccgagat cattgaagcg gttgaaaaaa
    18301 attgcttcca gaaaggttac accctgattc tgggtaatgc gtggaacaat cttgagaaac
    18361 agcgggctta tctgtcgatg atggcgcaaa aacgcgtcga tggtctgctg gtgatgtgtt
    18421 ctgagtaccc agagccgttg ctggcgatgc tggaagagta tcgccatatc ccaatggtgg
    18481 tgatggactg gggtgaagca aaagctgact tcaccgatgc ggtcattgat aacgcgttcg
    18541 aaggcggcta catggccggg cgttatctga ttgaacgcgg tcaccgcgaa atcggcgtca
    18601 tccccggccc gctggaacgt aacaccggcg caggccgcct tgccggtttt atgaaggcga
    18661 tggaagaagc gatgatcaag gtgccggaaa gctggattgt tcagggtgac tttgaacccg
    18721 aatctggtta tcgcgccatg cagcaaatac tgtcgcagcc gcatcgccct actgccgtct
    18781 tctgtggtgg cgatatcatg gcaatgggcg cactttgtgc tgctgatgaa atgggtctgc
    18841 gcgtcccgca ggatgtttcg ctgatcggtt atgataacgt gcgcaacgcc cgctatttta
    18901 cgccggcgct gaccacgatc caccagccaa aagattcgct gggtgaaaca gcgttcaaca
    18961 tgctgttgga tcgtatcgtc aacaaacgtg aagaaccgca gtccattgaa gtgcatccgc
    19021 gcttgattga acgccgctcc gtggctgacg gcccattccg cgactatcgt cgttaatcac
    19081 ccgttgcggg agtctcttcc ggctcccgca gccactcctt attcagcgtc tcactatcgc
    19141 cgagatactc aagcagccag gttaacgcag gcgacatatc attttgctgc catgtcagac
    19201 aacatgccga atccgggaag gggttttcca attcaagtgc tacccacttc ccctcattaa
    19261 gccacggttt ggcgaaatgt gttggcacca tccctatgca taatcctgcc gagatacagg
    19321 ttgccgatga ttcccagtca ggcacgacaa ctcttttttg gttatccagc aaccaggtaa
    19381 tacgtttagg tagcgttcgc gaggtgtctt cgcgcaccaa cgacggccag ttgcgcaacg
    19441 tatcatcgct gaatgggcca tccatcaacg ccagcgggtg gtggctggca acaacgcaac
    19501 tccagcttag catccccata tcccggaagg cataacgacc gcctaccgga atcgcgcgtg
    19561 ttgcgccaat cgccagttcc acgcgcccgt cggaaagcgc atcccagaca ccgttgaaca
    19621 cttcctgaaa gacaagaagt tcgacatcat caaaatggcg ataaaaatca acgatcatct
    19681 gccgtgtacg ttctggcctg acaatattat ccactgcgat agctaactga ccgcgccagc
    19741 cgttcgctat ctgctgacat tgctggcggg tgatctgcat ttttttgaca acagagcgcc
    19801 cttctttgag aaaccacgct ccagcagcgg tcagctccac atcacggtgc cgtcgttcaa
    19861 agagcggcac cgccagccac tcttccagct gacgcacggt atagctgacc gcagaaggaa
    19921 cgcgatgcag ctcctgtgcc gcagcgctaa aactaccatt acgcgctacc gcatcaacaa
    19981 cttcgagtga atattctgac cacatagtct gcctgcaaaa tttttgaaac cagtcatcaa
    20041 atattaccgt ttcacaacac taatttcact ccctacactt tgcggcggtg tttaattgag
    20101 agatttagag aatatacatg caacctggga aaagattttt agtctggctg gcgggtttga
    20161 gcgtactcgg ttttctggca accgatatgt atctgcctgc tttcgccgcc atacaggccg
    20221 acctgcaaac gcctgcgtct gctgtcagtg ccagccttag tctgttcctt gccggttttg
    20281 ccgcagccca gcttctgtgg gggccactct ccgaccgtta tggtcgtaaa ccggtgttat
    20341 taatcggcct gacaattttt gcgttaggta gtctggggat gctgtgggta gaaaacgccg
    20401 ctacgctgct ggtattgcgt tttgtacagg ctgtgggtgt ctgcgccgcg gcggttatct
    20461 ggcaagcgtt agtgacggat tattatcctt cacagaaagt taaccgaatt tttgcgacca
    20521 tcatgccgct ggtgggtcta tctccggccc tggctcctct gttaggaagc tggctgctgg
    20581 tccatttttc ctggcaggcg attttcgcca ccctgtttgc cattaccgtg gtgctgattc
    20641 tgcctatttt ctggctcaaa cccacgacga aggcccgtaa caatagtcag gatggtctga
    20701 cctttaccga cctgctacgt tctaaaacct atcgcggcaa cgtgctgata tacgcggcct
    20761 gttcagccag tttttttgca tggctgaccg gttcaccgtt catccttagt gaaatgggct
    20821 acagcccggc agttattggt ttaagttatg tcccgcaaac tatcgcgttt ctgattggtg
    20881 gttatggctg tcgcgccgcg ctgcagaaat ggcaaggcaa gcagttatta ccgtggttgc
    20941 tggtgctgtt tgcagtcagc gtcattgcga cctgggctgc aggcttcatt agccatgtgt
    21001 cgctggtcga aatcctgatc ccattctgtg tgatggcgat tgccaatggc gcgatctacc
    21061 cgattgttgt cgcccaggcg ctgcgtccct tcccacacgc aactggtcgc gccgcagcgt
    21121 tgcagaacac tctacaactg ggtctgtgct tcctcgcaag tctggtagtt tcctggctga
    21181 tcagtatcag cacgccattg ctcaccacca ccagcgtgat gttatcaaca gtagtgctgg
    21241 tcgctctggg ttacatgatg caacgttgtg aagaagttgg ctgccagaat catggcaatg
    21301 ccgaagtcgc tcatagcgaa tcacactgat ctatatcgat atacttatac ttaggctgct
    21361 aacaaaattt tgttgtatga ttgaaattag cggcctatac taatttcgag ttgttaaagc
    21421 tacgataaat attatgtttt tacggggaca ggatcgttcc cgactcacta tggatggtca
    21481 tttcggcaaa ggttcctcct ttccctctgt tctacgtcgg attatagact cgcggttttt
    21541 tctgcgagat ttctcacaaa gcccaaaaag cgtctacgct gttttaaggt tctgatcacc
    21601 gaccagtgat ggagaaacta tgagttcatc gtgtatagaa gaagtcagtg taccggatga
    21661 caactggtac cgtatcgcca acgaattact tagccgtgcc ggtatagcca ttaacggttc
    21721 tgccccggcg gatattcgtg tgaaaaaccc cgattttttt aaacgcgttc tgcaagaagg
    21781 ctctttgggg ttaggcgaaa gttatatgga tggctggtgg gaatgtgacc gactggatat
    21841 gttttttagc aaagtcttac gcgcaggtct cgagaaccaa ctcccccatc atttcaaaga
    21901 cacgctgcgt attgccagcg ctcgtctctt caatctgcag agtaaaaaac gtgcctggat
    21961 agtcggcaag gagcattacg atttgggtaa tgacttgttc agccgcatgc ttgatccctt
    22021 catgcaatat tcctgcgctt actggaaaga tgccgataat ctggaatctg cccagcaggc
    22081 gaagctcaaa atgatttgtg aaaaattgca gttaaaacca gggatgcgcg tactggatat
    22141 tggctgcggc tggggcggac tggcgcacta catggcatct aactatgacg taagcgtggt
    22201 cggcgtcacc atttctgccg aacagcaaaa aatggctcag gaacgctgtg aaggcctgga
    22261 tgtcaccatt ttgctgcaag attatcgtga cctgaacgac cagtttgatc gtattgtttc
    22321 tgtggggatg ttcgagcacg tcggaccgaa aaattacgat acctattttg cggtggtgga
    22381 tcgtaatttg aaaccggaag gtatattcct gctccatact atcggttcga aaaaaaccga
    22441 tctgaatgtt gatccctgga ttaataaata tatttttccg aacggttgcc tgccctctgt
    22501 acgccagatt gctcaatcca gcgaacccca cttcgtgatg gaagactggc ataacttcgg
    22561 tgctgattac gatactacgt tgatggcgtg gtatgaacga ttcctcgccg catggccaga
    22621 aattgcggat aactacagtg aacgctttaa acgaatgttt acctattatc tgaatgcctg
    22681 tgcaggtgct ttccgcgccc gtgatattca gctctggcag gtcgtgttct cacgcggtgt
    22741 tgaaaacggc cttcgagtcg ctcgctaaag gctattctat cgccccctct ccgggggcga
    22801 tttcagatca ggcttctgta cctggttgat tcatggcatt ttctcgtgcc gccagcacac
    22861 gttctaccgt atctaccact gcctgagttt gtggatcaat ttcaatgttg acgcgtgcgc
    22921 cgagtttttt cttcccaaga gtcgtgcgtt ccagagtttc cggaattaaa tggacgcaaa
    22981 aacgcgttgg cgtgacttcg ccgacggtca ggctaatacc gtcgatgcca ataaatcctt
    23041 tgtacaggat atatttcatc aactgactat cctggacttt aaaccagatc tggcgattat
    23101 tttccgaggt taatattttc gccacttcag cggtggtcat aatatgacct gacattaagt
    23161 gtccgccaat ttcatcactg aatttcgccg cacgctcaac gtttacccaa tcccccactt
    23221 ttaaatcgcc aagattggta atgcgtaacg tttctttcat caggtcaaaa ctgacatggt
    23281 tgccgttaat ttccgtcacg gtcaggcagc aaccgttatg cgccacggaa gcacctgttt
    23341 ccaggccgtc cagcatgtgg tcgggtaact ccaccacatg cgtacgaaaa tttggtttct
    23401 cgtcaatcga caccagtttt acggtgccct gtacaatccc cgtaaacata cttacaactc
    23461 ctgaaatcag ttaagacatt ctgttcagca caatagcagg tggcaaacgc ccttaccagt
    23521 gaaggggtaa gaatgactat tttttcactg gagaattaat aaatcctcgc tacaatagac
    23581 tgaatttccc ctgcttcttc tttttgctgc ccattcaggc ggctttttag tctctcatat
    23641 aactacaaat aaaaggtgtt cacgtgcaga agtatatcag tgaagcgcgt ctgttattag
    23701 cattagcaat cccggtgatt ctcgcgcaaa tcgcccaaac tgcgatgggt tttgtcgata
    23761 ccgtgatggc gggcggctat agtgccaccg acatggcggc ggtcgctatc ggtacttcta
    23821 tctggcttcc ggcgatcctc tttggtcacg gactgctgct ggcattaacg ccggttatcg
    23881 cgcaattaaa tggttccggt cgacgtgagc gcattgcgca tcaggtgcga caaggtttct
    23941 ggctggcagg ttttgtttcc gttctcatta tgcttgtgct gtggaatgca ggttacatta
    24001 tccgctccat ggaaaacatc gatccggctc tggcggacaa agccgtgggt tatctgcgtg
    24061 cgttgttgtg gggcgcgccg ggctatctgt tcttccaggt tgcccgtaac caatgtgaag
    24121 gtctggcaaa aaccaagccg ggtatggtaa tgggctttat cggcctgctg gtgaacattc
    24181 cggtgaacta tatctttatt tatggtcatt tcggtatgcc tgagctcggt ggcgttggtt
    24241 gtggcgtggc tactgcggcg gtgtattggg tcatgttcct tgccatggtt tcttacatta
    24301 aacgcgcccg ctccatgcgc gatattcgta acgaaaaagg caccgcgaaa cccgatcctg
    24361 cggttatgaa acgactgatt caactcggtt tgccgattgc gctggcacta ttctttgaag
    24421 tgacactgtt tgccgtcgtg gctctgttag tgtctccgct cggtattgtt gatgtcgcag
    24481 gacaccagat tgccctgaac tttagttcac tgatgtttgt gcttccaatg tcgctggcgg
    24541 cagcggtaac tatccgcgta ggttatcgtc tgggtcaggg ctcaacgctg gatgcgcaaa
    24601 ccgctgcgcg gaccgggctt atggtgggtg tctgtatggc aaccctgacg gccatattca
    24661 cggtttcact gcgggagcaa atcgccctgt tgtacaacga caatcccgag gttgtaacgc
    24721 tggctgcgca tttgatgttg ctggcggcgg tatatcagat ttctgactca atccaggtga
    24781 ttggcagtgg gattttgcgt ggttataaag atacgcgttc cattttctat atcaccttta
    24841 cggcttactg ggtgctgggc ttgccaagcg gctatattct ggcactgacc gatctggtcg
    24901 ttgaacctat ggggccagca ggcttctgga taggctttat tattggcctg acgtcggcag
    24961 ccattatgat gatgctgcgt atgcggttcc tgcaacgtct gccgtcagtt atcattctgc
    25021 aacgagcatc ccgctaataa agacaaggcg caaccttcac gggttgcgcc tgtgttttta
    25081 cgcaggctgg agcgttgcgc caatcccgtc ttcgtctggc tgtaatttca gagcgttgca
    25141 cagagtttgc cgactggcat ctgttatctc aacgtgtata tccccggtaa cttccccttt
    25201 cacattgatg aagtcaggtg agatttcttt cgcactggtt tgcaggcaaa tcgctccctc
    25261 tccgcgcagg cgtttcactt tcagttgcac atgcgttgcc gcaccagtga tcagccacag
    25321 ttttccgcca ttcagattga tgttaccaac aataaaagtg acaccgtcac cttttaccgt
    25381 actgccgtcg agaatcaaca caccgccctg ctgtacagta atcgttgatt gttgcagatc
    25441 tgcgccatag ccaataatca ttgaagcgcc gtccagtaat gcaacactcc cggtcagttc
    25501 aaccggtgcg cgatcttcct ctacgacaaa attttcgtcc gcgctggtgg gtgacaacag
    25561 gatcactgtt ccaccttgta ttaccgcatt ttccagcgta ccggcatgca cttcagcccg
    25621 accacccaca ttcacggaca ggttttgcgt cttaccggaa ctgtagagct gaaggccatc
    25681 cgtccccaga cgataaatgg atccattctc aacaatagtg tcagagtctt caccaccgag
    25741 attattcatg gtgccaccag caagcaacgt atcactggct ttcccggcca gcataaaaac
    25801 gccgccctga ttaatctcac aattgctggc ttccccacct ggatacacca acaattcacc
    25861 accttcatct accacgaccg ctgaggttgt cccattaacc aacaggccgc cctcttgatc
    25921 gagaatgatt ttttccgcgc gatgtccttc cagtacacgc aggttaccgc cattttccag
    25981 caacaaaccg caggcataac cctgatcaac gctgaattca ccttcgggat ggcggccatt
    26041 aaccgtagcg agcgtagaga gactaattgc gcctccctcg gactgaacaa tatctgtggc
    26101 aatggcaccg tcgctgaccg atagcgtgcc attgttaccg tactgtgtgc atttggcgat
    26161 cccaccggtt gccactttca accagccagc ccccgtgacc acggtatttt cggctcttcc
    26221 gccagcttca acaatcaact cgccttcagt aagttgcgtc gcgccaatga cctcgccggt
    26281 cttaacaatt tgcacattcc cgtcgctcat caagtttttc gcatcagatc ccatgattta
    26341 ttcctttgct gcatctgtgt gcctttattg ctacctaagt gtaaaggcta cggaggattt
    26401 atccacgaca gatttgagat ggtggcaaac aactctgttt aaactctgat acacgaatta
    26461 ttgggttgta tcagatgtaa atgcgatcct gaataaaaat cgcccttgca aatcaacaaa
    26521 atatagccaa tttgcttaaa agctctgcaa acggtcgaaa tagcgcagaa aattacgttt
    26581 tgcctcttgc caccttccca ctctgccgct aatattcgtc cccgttgtca cctacaacgt
    26641 tgcgttcata gctcagttgg ttagagcacc accttgacat ggtgggggtc gttggttcga
    26701 gtccaattga acgcaccatc ctgcgtccgt agctcagttg gttagagcac caccttgaca
    26761 tggtgggggt cggtggttcg agtccactcg gacgcaccag attttcttaa tctggtcttc
    26821 tcctttttcc ctctgtttct tctctgtatc caatacgtta aaagatttac actgtcttcg
    26881 tatgcgttat cagaaggaga atcgctatgg caactttgtt acaacttcat tttgctttta
    26941 atggcccatt tggcgacgca atggctgagc agcttaaacc gcttgctgag tcgattaatc
    27001 aggaacctgg ttttctgtgg aaggtatgga cagaaagtga aaagaaccac gaagccggtg
    27061 ggatctatct ttttactgat gaaaaaagcg cccttgccta tctggaaaaa catactgccc
    27121 gacttaaaaa tctcggcgtt gaggaagttg tcgccaaagt tttcgatgtc aatgagccac
    27181 tgagtcaaat caatcaggca aaactcgcct gacagaattt aatcaagggc ggttagcgcc
    27241 cttttcatcc ctgtctgaaa tttctcaaat tctaaaaatc tcaaccaaac ttatctgata
    27301 acactaaatt cgaaagaatg cgtacaggta agtaacaatg aaaaaaattg ctattgtggg
    27361 tgccgggcct acggggatct acaccttatt ctcgcttcta cagcaacaaa ctccactttc
    27421 tatttctatc ttcgagcagg ctgacgaggc cggtgtcggg atgccataca gtgatgagga
    27481 aaactcaaaa atgatgctgg caaatattgc cagtattgaa ataccgccga ttaattgtac
    27541 gtatctcgaa tggctacaaa agcaagaagc cagccatctc cagcgttatg gcgttaaaaa
    27601 agaaaccttg cacgatcgtc agtttttacc gcgaattctg ctgggcgaat atttccgcga
    27661 tcaattttta cgattagtag accaggcacg acagcaaaaa tttgcagtgg ctgtttatga
    27721 atcatgccag gttaccgatc tgcaaattac aaatgctggc gtcatgctcg ctacaaatca
    27781 ggatttaccc agcgagacgt ttgatttagt ggtgattgcc acgggtcacg tctggcctga
    27841 tgaagaagaa gcaaccagaa cgtattttcc cagtccgtgg tcaggcctga tggaagcaaa
    27901 ggtcgatgcg tgtaacgtgg gtattatggg aacatccttg agcggactgg atgcggcaat
    27961 ggcagtggct attcagcatg gttcgttcat tgaagatgat aaacaacacg tcgtttttaa
    28021 ccgcgataac gcaagtgaaa agctaaatat tacgttgatg tcgcgcacgg gtattttacc
    28081 cgaagccgat ttctattgcc ctattcccta cgagccctta cacatcgtca ccgatcaggc
    28141 attaaatgct gagattcaaa aaggtgaaga gggccttttg gatcgggtat ttagattgat
    28201 agtagaggaa atcaagtttg ctgatccaga ctggagccaa cgcatagcct tagagagcct
    28261 gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc aaacaacgcg acccatttga
    28321 ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa cgagaaaaac atactgttcc
    28381 ctggcgttat gtcattctgc gcctgcatga agccgtacag gaaattgttc cacatctgaa
    28441 tgaacacgac cataaacggt tcagtaaagg ccttgcccgg gtttttatcg ataattatgc
    28501 ggcaatccct tcagagtcta ttcgtcggct gctggcctta cgtgaagcgg ggatcattca
    28561 tattctcgcc ctcggtgaag actacgaaat ggaaattaat gagtcgcgca ccgtcctgaa
    28621 aacggaagac aacagctact cgtttgacgt ttttattgat gcccgcggac agcgtccgct
    28681 taaagtgaaa gatattcctt tccctgggct acgcgagcaa ttacagaaaa caggggatga
    28741 aatccctgat gttggcgaag attatacgtt acagcaaccc gaagatattc gtggacgcgt
    28801 agcgttcggc gcgttgccct ggttgatgca cgatcagcct ttcgttcagg gacttacggc
    28861 atgtgcagaa attggtgagg cgatggctcg agcggtcgta aaacctgcat cccgtgcacg
    28921 tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac tcaacaaact gatgctgttc
    28981 attaagacga tagcggactc cggctcgaat attgttttca ccttcaatgg cgacggcgaa
    29041 gcgaactctt tcgccgtcat gatatgccag tgcggcgctt ccccctggcc cgagaataat
    29101 tgagtcgacc acgcccgtgc tcactaccgt tgaattttca ccgctggcaa tgatgtgtac
    29161 attgtcgccg ctgttggcaa ttctggcgtt tgcgccaaag ctggcgattt gcgccaggtc
    29221 accattactg gcgacatgac accgttcacc caatgtacaa acgcgtactc gcattccggt
    29281 gttcgcaatc cgactactat ctccagcgct gcttatccgc gaagaattac cggttacagc
    29341 aacccgtgcg cgttcaccgg agctgccaat gtgactgtta aagccgacac ttccaatccg
    29401 cacgctataa cccatactgg caatttgcgc ggcataccca gcggttgcga ttcgggcgtt
    29461 atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg gcaaactgac tgcaaaatgg
    29521 tatctctccg aggtctgtgg ccagtttttg catagaggaa acttcctgat gcgcgaagtt
    29581 ttcatccgcc agccattttt tccacgcata ctcaaccaga ctctccgccc agtccgtata
    29641 tccttcctca atcagtgcat gatgaatatc ggcataacta ccgccgcgtg ggaaatggcg
    29701 caggaaccag cgatacatca ccgctccgat acgccattct cgcaggtctg ctcgtgtaat
    29761 gatcattcgt tagtgccggt gatagccatc gaccatgctt ttaaaggttt cgtgtggcgt
    29821 acgccccgtg gtgcaaagat aaagatgacc gaagataaaa aagaggctta taaatgccag
    29881 agcaaaatgc gcctgcaata accagtatct tacgccagga aacacatctc ccacggcttg
    29941 cggatagaga cacagcagcc ccgtcaatag taacaacggc agcaatccat acataacacc
    30001 aacataggcg acctgctgta agggattaaa tttagactgg gttgttgccg ggaaaggatg
    30061 ttcttccccc tgcataatgc caaacaaata aaatcgcgtt tgttttgccg ctcgctccag
    30121 ccacccctga cgacgaatgc gatagtggtg accattaccc ccaacggcat tgatcagcac
    30181 aaagccgagc cagcatgcga gtaacaaaaa tccgcaaact tcatgcaccg caaccagact
    30241 tttaaccgca gttgcgccca ccagcgcaaa gtgatttatc agcccacttg ccagcaacaa
    30301 tacaaagagt aacgcattcg accagtgcca taatctgacc gctttgcagt aaagataaat
    30361 cttctcacca taatcggtga ctgatttctt cacgccacga gcacgaagca aggcatgcag
    30421 ccccagcacc aaccacatcc caaccagcaa tactccggca atgatcaacc acaccggcca
    30481 aaattcggga gtgaactgtg gcacatagtt cgctaactgg ctctgaaact gttcagcatg
    30541 ttgcgacggg ttcatacatt ttccttttta atcaaatgtt gaccgaaccg gcgatacaga
    30601 tgcggtttcc cggcaccagg tagctgatat tgatagtatt tattttgctg caaccacgcc
    30661 tgaatttctg ggctatcttc gcgaccaaag atcaatgcgt gttccggaca ggcgctgacg
    30721 caaatgggcg gaaagccttt tgccagtcgg gactcagcgc agaaatcaca tttatctgcc
    30781 actttagtga cagggttaag gtagcgaacc tggtacggac aagcgccaat gcaataactg
    30841 cagccgatgc actgcgattt ttctacccgc acgatcccct gctcatcacg ccacgacgcc
    30901 cctgtcgggc atacgtcgat gcacggagca tcttcgcagt gctgacatga ctggcgaaaa
    30961 aagtgatact gcgtttcgtt gtcgttatcg gtgactggaa tgtgtgctat cgataaacgg
    31021 cttccctgag cggggacgtg gttagtttta cggcatgcgc gggcgcaaat attacagcca
    31081 ttgcaccgtg actcgtcatg aatcatggca taccgaactt ccttttcttg cctcgtgttc
    31141 gctaacagag aagacgcaga accagtaaaa aagattactg ttcccatccc cagaacaaat
    31201 ttgcgtcgag tgaaggacat ctgattatcc cctaatgcag tggctgcaac tgtccaccct
    31261 atccatgaca tctccctgaa atttcagggg ttctgaaact gctctacctc gaaccattcg
    31321 cataagccat aagccttgcc ataggtcata aatcgctcgc taaacaagta caacaccgcc
    31381 gtcgatgact ccacctgccg tatatcggca tattcaggtg caacatccat cgcggcgatc
    31441 gcggcttcaa tctgcgcttc ctggaagtaa tatggcgctt gcatcagcat ggcgactttg
    31501 taaggacgag gatacgtctg gcactcaaaa cgaacggcgt gtgcaatggc gcgacaaatg
    31561 tcctgctcca caacttgcaa agacatcgcc gcgtagtttt cactcatggc ctgcgttgag
    31621 taatagtaat catcctggct tcccgtcata caagcgatat ccgcgtagtc tccgttctgc
    31681 ttaatgcttt ccagcaaaac tgacagctcc tcttctgcaa ccgaataggg tggcgagaga
    31741 aacacggcgc gcgccaccag ctgcccagcc gcagaatgct ggcgaatgaa ctctgccagg
    31801 atttcagccg gttctggttc ggttaccgta tcaacggggt gacgcatccc ctgtaaccat
    31861 tgccgcttcg cctcgtcaac ttcgctgact ttcgctaacg gcaattcatc ccgatggttc
    31921 atctcaccca ttccttacac aggcagtaga ttgtgtgctg ccagatcggc ggcaatatct
    31981 tccagcccca gacgctgcaa tgtttcgcgg gtcggacaac caagctgtgg gtcccagccc
    32041 atttctttgt agaacatcgt gagcgaagca tgcatatcgt cacgatccat tttgtcagta
    32101 ccttcagtaa agaccgggat ctgcggatcc ttgtcgaata cccaggaaca gataagatcg
    32161 tgttcattac gcatatcttt ggtttgcatc agttttaccg tgtaggcacg atgcaacgta
    32221 aagatacgct ctgcagctaa atctaatttt tcctgagtca tctcttcgcc ggtgatcgct
    32281 ttgaagaatt tggcttcaag cgccagatcg ccccggtaat tacggctttt cagcggcgaa
    32341 acggtcattg gccagaccca gttgcacagg gtgacggcgt tatgcaaaca gacccgcaac
    32401 agcgaccact ttgcatattt aatttttgcg tcattgattg gcgtgtagtt tttggtttca
    32461 tcgtaggcat cttcagaacc aaaaagctct ttcgccactt cacgctgcag tttcaatggc
    32521 aaacccgaac caataaaatt gatatgggta tgcgtcatgc agtcacggtt gaacatacag
    32581 ttaacaatgg aaccgacctg cgctgacgct tcattggcat ggtgaaccgg atagccaaac
    32641 ggcgaccaga gtttattttt tgcgtagccc cagtactctt cacccaaatt ccagcgttct
    32701 gcgatggcat atgaaccatc agccaggtga ctcagctcac ccacacgatg cgccagacgg
    32761 tagtaaaaat ctttaatgaa attaacgtca cccgcttcca gttgatccca gtgaatttcc
    32821 gcatactctt cagctggcag aacacgcttg aacacccctt tgctgtaaca ataagtaaag
    32881 tcgcgatgca gctgcccgta gttacaccat aggccgtagt catcgaacaa attcagacca
    32941 accaggttac caatcacacg accatcgtct ttatcttcaa aatctttcgg cccgttcggg
    33001 aagatggtgg tatggacaaa gttggctaca caagtgttac cacctgtgct gggcacgcca
    33061 aactctttca cccgaggaat attcatttgg gtcatacaac ggatcgggca agagtggcag
    33121 ccactcattt ttactgtgta tttctctgcc gccggaccta agtcaaaaac ggatttatag
    33181 gtacgaaagc cgaccgtgtt ctgattgcct ggcggaattt cccccgtttc aatcggacca
    33241 ccttcagccg cgccccaaaa cagctcttta cgtgctgtcc aacgtgactt gggatctgaa
    33301 tactctgccc acgattgcgg cgtacttggc acgacatggt tgttattcgc accaataagt
    33361 tcagtcatca tgtaatcgtt gagacgcttc atctcctgac gatcggcaat gttgacccct
    33421 ttcgtccctt caaccgcaat cgctttcagg tttttcgaac ccataattgc gccagttccc
    33481 gcaccgccgc tgtggttacg gctattcaac atgccagaga gaggaacaag gttttcccca
    33541 gcctgaccaa tagccgccac acaggtttcc ggactggtca atcgacaaat ttcttccgtc
    33601 gttgcgcgcg tcccttttcc ccataagaaa tcggcttttt ccaggctaac tttgtcatct
    33661 ttaatcttca gccataccgg tgatttcgcc ttcccttcga taataatgac gtcgtatcca
    33721 gcgaatttca tttgcgctgc aaaaaagcca cccatatggg catcgacaac taaatttcct
    33781 ttggtaaaag tagaaagtga ggtgatattt acgcgagaac tacagggggc accagatcca
    33841 gttaatgggc cggtagcaaa gactaattta ttcgcttcat cgaaaggttt cgtgcctggc
    33901 ggtacttcgt catacataat tttgtagccg aagcccatgc caccgacaaa acttttaaac
    33961 ttactggaat cttcgagggt aatatttcct gtcgtgagat tgactcttaa tatattacct
    34021 gtccaaccgt tagccatgat tttttccttt gcaagattca cacagtaata tctttccact
    34081 cgataatttt taacgcccct gttgggcagg cgtttgcgca ttcaccgcat aacacacatt
    34141 tcgaggattt tttactttcg gtatttacgg tggccatcat ccacgggcat gccgtagtac
    34201 aggcgctaca gccaatacag cgtttatgat cgacggtaat acagccttct ttctgctgcc
    34261 aggtaatcgc accaatcggg cagacgttca tgcattgcgg ctctttgcat tgacggcagg
    34321 tgtccgcggt atagttgaga tcgccataca ggccgccgcc agagccaacc ccgttgtcgc
    34381 caaagaaata attgcgatgg attttgatac gggagaagaa tgtccctact gagccatcgt
    34441 tgaagttggt acaagagatt tcacagcggt ggcaacccgt gcatcgcgct cgctgggtca
    34501 ccagcacccc tttgggtgta tttatcaacc ctactgtgcc gctatcaata tcttcctgtt
    34561 tgcaacccaa aagtgacagt aacgcagggg caattgttag accagcaagg ccttttcctg
    34621 atatacgcaa gaattctaat cgcgttaagc caatatctaa tagtggacga tcaaccgggt
    34681 tcatttattt cgcatcctct tcgcaaacac agtgctgaca attcagcagc ctgatattaa
    34741 gcattcaact aatacgctgc tgactctgtc cccaacacca gatattttcc ctaacccgat
    34801 agtgtatgag aaaagattta acgccgataa actaatcctt tatggttagg agaaaaaata
    34861 atgtgattat tctgagccct taacattgat cgttatc
//
LOCUS       DAFNBJ010000033        34631 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000033 DAFNBJ010000000
VERSION     DAFNBJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.033"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(73..1326)
                     /gene="amiC"
                     /locus_tag="L2X49_001564"
     CDS             complement(73..1326)
                     /gene="amiC"
                     /locus_tag="L2X49_001564"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBN4796667.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1558..2889
                     /gene="argA"
                     /locus_tag="L2X49_001565"
     CDS             1558..2889
                     /gene="argA"
                     /locus_tag="L2X49_001565"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBN4796668.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2951..4777)
                     /gene="recD"
                     /locus_tag="L2X49_001566"
     CDS             complement(2951..4777)
                     /gene="recD"
                     /locus_tag="L2X49_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBN4796669.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4777..8319)
                     /gene="recB"
                     /locus_tag="L2X49_001567"
     CDS             complement(4777..8319)
                     /gene="recB"
                     /locus_tag="L2X49_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBN4796670.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8312..11200)
                     /gene="ptrA"
                     /locus_tag="L2X49_001568"
     CDS             complement(8312..11200)
                     /gene="ptrA"
                     /locus_tag="L2X49_001568"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBN4796671.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11376..14744)
                     /gene="recC"
                     /locus_tag="L2X49_001569"
     CDS             complement(11376..14744)
                     /gene="recC"
                     /locus_tag="L2X49_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBN4796672.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKSHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14757..15080)
                     /locus_tag="L2X49_001570"
     CDS             complement(14757..15080)
                     /locus_tag="L2X49_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBN4796673.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15065..15472)
                     /locus_tag="L2X49_001571"
     CDS             complement(15065..15472)
                     /locus_tag="L2X49_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBN4796674.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15469..16032)
                     /locus_tag="L2X49_001572"
     CDS             complement(15469..16032)
                     /locus_tag="L2X49_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBN4796675.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16023..16493)
                     /locus_tag="L2X49_001573"
     CDS             complement(16023..16493)
                     /locus_tag="L2X49_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBN4796676.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16678..17472)
                     /gene="thyA"
                     /locus_tag="L2X49_001574"
     CDS             complement(16678..17472)
                     /gene="thyA"
                     /locus_tag="L2X49_001574"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBN4796677.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17479..18354)
                     /gene="lgt"
                     /locus_tag="L2X49_001575"
     CDS             complement(17479..18354)
                     /gene="lgt"
                     /locus_tag="L2X49_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBN4796678.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18505..20751)
                     /gene="ptsP"
                     /locus_tag="L2X49_001576"
     CDS             complement(18505..20751)
                     /gene="ptsP"
                     /locus_tag="L2X49_001576"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBN4796679.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYADAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20764..21294)
                     /gene="rppH"
                     /locus_tag="L2X49_001577"
     CDS             complement(20764..21294)
                     /gene="rppH"
                     /locus_tag="L2X49_001577"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBN4796680.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            21979..22668
                     /gene="mutH"
                     /locus_tag="L2X49_001578"
     CDS             21979..22668
                     /gene="mutH"
                     /locus_tag="L2X49_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBN4796681.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22737..23450
                     /locus_tag="L2X49_001579"
     CDS             22737..23450
                     /locus_tag="L2X49_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBN4796682.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23588..23806
                     /gene="ygdR"
                     /locus_tag="L2X49_001580"
     CDS             23588..23806
                     /gene="ygdR"
                     /locus_tag="L2X49_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBN4796683.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23914..24954
                     /locus_tag="L2X49_001581"
     CDS             23914..24954
                     /locus_tag="L2X49_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBN4796684.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24986..26179)
                     /gene="lplT"
                     /locus_tag="L2X49_001582"
     CDS             complement(24986..26179)
                     /gene="lplT"
                     /locus_tag="L2X49_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBN4796685.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26172..28331)
                     /gene="aas"
                     /locus_tag="L2X49_001583"
     CDS             complement(26172..28331)
                     /gene="aas"
                     /locus_tag="L2X49_001583"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBN4796686.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28917..29948
                     /gene="galR"
                     /locus_tag="L2X49_001584"
     CDS             28917..29948
                     /gene="galR"
                     /locus_tag="L2X49_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBN4796687.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29955..31217)
                     /gene="lysA"
                     /locus_tag="L2X49_001585"
     CDS             complement(29955..31217)
                     /gene="lysA"
                     /locus_tag="L2X49_001585"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBN4796688.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDVGFNDLMRPAMYGSYHHISALAADGRSQEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31339..32274
                     /gene="lysR"
                     /locus_tag="L2X49_001586"
     CDS             31339..32274
                     /gene="lysR"
                     /locus_tag="L2X49_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBN4796689.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32261..32962)
                     /gene="ygeA"
                     /locus_tag="L2X49_001587"
     CDS             complement(32261..32962)
                     /gene="ygeA"
                     /locus_tag="L2X49_001587"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBN4796690.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGAQGVIFGCTEIGLLVPEERSVLPVFD
                     TAAIHAEDAVAFMLS"
     gene            complement(33091..34509)
                     /gene="araE"
                     /locus_tag="L2X49_001588"
     CDS             complement(33091..34509)
                     /gene="araE"
                     /locus_tag="L2X49_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBN4796691.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVRLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8456 a         9512 c         8588 g         8075 t
ORIGIN      
        1 tattgaacac cctaacgggt gtttttttgt ttctgctctc ccataaaaaa agcgccattc
       61 agcgcctttt tatcatcccc ttctcgccag cgtcgcccca tcggcaaaat acgctttaat
      121 tcccgcaaga atagactccg caacttcctg ctggaaagtc gccgttttca gtttacgctc
      181 ttcctcaacg ttactgataa acgccgtttc gaccagaatg gagggaatat caggcgcttt
      241 cagcacggca aacccggcct gttcaacctg atttttatgc agcttgttga ttttaccgag
      301 cttattcagc accgctttac caaacttaag gctgtcggca atggtcagcg actgcaccat
      361 atcgaacatg gtgtggtcga catagcggtc accgcttttg ctcacgccac caatcaagtc
      421 cgaggcgttc tgggtctgtg ccagatattt tgccgcagta ctggttgcgc ctttggttga
      481 gagcgcaaac actgaggaac cgctcggctg gcgactggta aaggcgtcgg catggataga
      541 gacaaacagg tcggcacgct gtttctgggc ttttgctacg cgcacttgca acggaatgaa
      601 gatgtcttca ttgcgcgtca tgtacacctt catattgccc tctttctcga tcagagagcg
      661 cagacggcga gctatttgca ataccacgtc tttttcgcgc gttttgtatt tccccaccgc
      721 accggagtct tcgccgccgt ggccagggtc aagcataata acaataggac gatcgcgccc
      781 tgctttaccc ggttgtggac cactttgtgc tggcggcacc tgcttttcga ggtcgccttt
      841 gttgtaatcc tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat tggccggata
      901 gaggtccatc accagacgct ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg
      961 ttttacgttt tgctttaatt caaaaaccat ccgtacggtc tgcgggtcaa attgcccgac
     1021 gcgcgccgac ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac
     1081 cgagttcagg tttacatctt cgatatccac caccacgcgt tccgggttac tcaacgcgaa
     1141 ctgcttatat ttcagctgac gatttgattc taccgtcacg cgggtgtagc tggacgcagg
     1201 ccagacgcgc accgccacga cctggctaac cgcagccagg ctgacctgac ttacgctcaa
     1261 tagccacata gcgcccgcgc cttgcagtaa acgacgacgg ctgattgcag tgttggatcc
     1321 tgacatgcct ctcccgagca aaagaaatct aagctgtgta acaagtaaac gactaatttg
     1381 accggaaact ttagcgaaag acgcataatc tgtcatctaa taaacggtaa acattctttt
     1441 tatattcacg gcattactga taaaaaagtc gctctcgcat aaaatttaca cttgcaccct
     1501 gcgaaaaaac agaataaaaa tacaataatt tcgaataata atgcaaaggg gtgtgccgtg
     1561 gtaaaggaac gtaaaaccga gttggtcgag ggattccgcc attcggttcc ctatatcaat
     1621 acccaccggg gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga gcatgagaat
     1681 ttctccagta tcgttaatga tatcgggttg ttgcacagcc tcggcatccg tctggtggtg
     1741 gtctatggcg cacgtccgca gatcgacgca aatctggctg cgcatcacca cgaaccgctg
     1801 tatcacaaga atatacgtgt gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg
     1861 ggaacattgc aactggatat tactgctcgc ctgtcgatga gtctcaataa cacgccgcta
     1921 cagggcgcgc atatcaacgt cgttagtggc aattttatta ttgcccagcc gctgggcgtc
     1981 gatgacggcg tggattactg ccatagcggg cgtatccggc ggattgatga agacgcgatc
     2041 catcgtcaac tggacagcgg tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact
     2101 ggcgagagct ttaatctgac ctcggaagag attgccactc aactagccat caaactgaaa
     2161 gctgaaaaga tgattggttt ttgctcttcc cagggcgtta ctaatgacga cggtgatatt
     2221 gtctcagaac ttttccctaa cgaagcgcaa gcgcgggtag aagcccagga agagaaaggc
     2281 gattacaact ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc
     2341 gtgcgtcgct gtcatttaat cagttatcag gaagatggcg cgctgttgca agagttgttc
     2401 tcacgcgacg gtatcggtac gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca
     2461 acaatcaacg atattggcgg tattctggag ttgattcgcc cgctggagca gcaaggtatt
     2521 ctggtgcgcc gttctcgcga gcagctggag atggaaatcg acaaattcac cattattcag
     2581 cgcgataaca cgactattgc ctgcgccgcg ctctatccgt tcccggaaga gaagattggg
     2641 gaaatggcct gtgtggcagt tcacccggat taccgcagtt catcacgggg cgaggttctg
     2701 ctggaacgca ttaccgctca ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc
     2761 acgcgcagta ttcactggtt ccaggaacgt ggatttaccc cggtggatat tgatttactg
     2821 cccgagagca aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt gatggcggat
     2881 ttagggtaag cgatgaaaat tgtcggatgc gacatgcgta acactcgtgc gtcgcatccg
     2941 gcaattacgt ttattcccgc gaactaaaca acgccgccag accactgcgc cgctcagtac
     3001 gagtggcgat tgccgcactt aatatacgct catcggcata cagcgacaga cggcgacgcg
     3061 cgcgggtcac cgcggtgtat accagctctc gcgttactac cggcgtgcgt tggctcggta
     3121 aaatcaacgc cgcatggtcg aactccgatc cctgcgattt atgtaccgtc atcgcccacg
     3181 tagtttcgtg ctctggcaag cgactcggtt gcacagactt aatattgccg tccggcatcg
     3241 caaaccagac gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg ccattaaaca
     3301 acccaagcgc gctgtcatta cgggcaatca ttaccggtcg accttcgtac caacgagagt
     3361 gcggatgacg atgaattttg cgcttctgtt gcataaactg ctcaattcgc tcattcagtc
     3421 cagccacgcc aaacggccct tcccgcaggg cgcacaaaag ttggtactca ttgaacgcct
     3481 gaatgattaa atccggctcg gcacgcgctt gcagcagatc cagataacgt ccataacccg
     3541 caagagcttc ctcaagcatc gcaatataat cttctccgct ctgtaaaagc cgtttttcga
     3601 tatcagtaaa atcctgctga aaaacggttt tcactgccgt tttatcacca cggttgatcg
     3661 ccgcagctaa ctgaccaatg ccagaatcac tgccgaaacg atagcttttt tgcagcaggc
     3721 agagactgtc gcgcaaagat gccgcttctg tgccagttcc tgccggaacg tgagttcccg
     3781 tcaggcggct tagctgcccg gcacgctcgg cggtaaagcc ggcattggca taagcgcaga
     3841 tatcgcccag cacagcccca gcctcaaccg aggccagttg atcacgatcg ccgagaaaga
     3901 tcactcgcgc atgatcgggc aaggcgtcga tcagtctcga catcataggc agatcgatca
     3961 ttgacgcttc atctaccacc agcacatcaa gatgcagcgg gttaccggca tgatgacgta
     4021 aacgctggct acccggctgc gcacccagca atcggtgcaa agtactggca tcttccggaa
     4081 tgcgtttctt ttgttcatcg gtcagcggta actgtcgcaa agccttgccg agagattcgg
     4141 ttaagcgcgc ggcagcttta cccgttggtg cagccagacg gatacggcag cgttcgccgt
     4201 cggccatttg aattaacgct gccagtaact tcgccacggt ggtcgtttta ccggtgcctg
     4261 ggccgccgga aatcaccgag atccgccgcg tcagcgccac tgccgctgca actttttgcc
     4321 agttaatttc atcgcttact ggaaaaagtt tgtccagggt ttgcgccagt agagcttcat
     4381 caacctcaat ggcatgattc acctcattga agaagcgcgc taccgtccgc tcgttacacc
     4441 acatgcgatt caaataaaga cgatcgccac agaggatcat cggcgtgggt tcatctcccc
     4501 tgctgaccgc ttgagaagcc agcaagcatt cttcccaatt ttgtagctca ccgatttcac
     4561 tgacacaggt cgccaacagc ggatgcgacg cctcgttatt ttccagtcgt gaaagcggca
     4621 aacagacgtg tccctctccg gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag
     4681 gatgttcatc tcccgccacg gtcagggcaa attgcacatc cagcgggcgt agctgtttgt
     4741 gctccacagc ttccagtaat tgcttttgca atttcattac gcctcctcca gggtcatacc
     4801 ggcaaacatc tcatccatca gggcaatcaa cccggcgttg ggtcgggttg tgtaaatccc
     4861 ttgttgcgga tgttctttat caacgccacg caggaacaga tagataacgc cgccaaagtg
     4921 acgatcatag tcgtaatcag caatgcgatg gcgcagataa cgatgcagcg ccagggtata
     4981 aagctgatat tgcagatcat agcggtgtgc ctgcatcgct gctgccatag cctgttgggt
     5041 gtaagccgaa ctgtcttcac ccagccagtt ggatttatag tcgagcaggt aataacgccc
     5101 ttcgtggcgg aataccaggt cgataaagcc ttttaacatg ccgcgtacct gcatgaactc
     5161 cagcggcggg cagccagcgg atagcgggtc aaactggcga attagtgcat caagctggct
     5221 ggcgatgagc ggttcgctaa tcggcaggta aaactccatc tccacctgtt tatcgcggtc
     5281 ggaaagctga ctcaggctaa cacccgtttc attgagaggt gcctggagga cagccgtgat
     5341 ccactcggtc aacaccggtt cccactgcga ttcaaagccg ccgagttcca gtttttcctg
     5401 tacccagttc gggtcaaccg gctgggtaaa atcgaggtct tcaaacaaac tgtgcaagaa
     5461 tgtccccggt gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac
     5521 gctgaccacg cctgcggcat caacatccag ccgtggcatc agatcctgag cgataccgtg
     5581 accacgctgc tgcaagccgg agtagctggt gacacgccag ttatcgccgg gcaatcgttg
     5641 tagtgtcctc gcattcagtt ctgcagttaa agcatcatta acctgccagg gctggttatc
     5701 accagtttgt gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg
     5761 aagccctgcc gcatcttgcg gttccccttt ttgcagcaaa cgcccgagcg cactttggtg
     5821 gacgtcggtg tcaccttttt tatcgccacg acggcgcacc agcggtgcaa cgccgagact
     5881 gcaatgccaa accgaacgcg tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg
     5941 ttcgacctcc gcgaggtcga cgctttctgg cgcagcgtta agatccaaaa ctgcctcaaa
     6001 cgagtggcga tcgtgataaa acgcctgatc ctggacgcgg aaattggtga taaacggcag
     6061 ccagaccaat ggatattcca gccctttcga tttgtggatc gtgacaatct gcaccagatg
     6121 tttatcactt tcgagacgca tttgttggct ggaggcatta ctgtctggct cgaggatatg
     6181 ttgcgataac cagcgtacca gcgcatgttc actttccagc tgcgttccgg cttcttgtag
     6241 cagttcgctg atatgcaaga tatcggtaag acgccgctca ccgcctgccg ttgccagcaa
     6301 gttttcagca atgttacgcg ccgacatcag cgcccgcagc atcggcataa cgccacgttt
     6361 gcgccagatt tgccgataac catcgaactc ttcgactacc acatcccacg catgttcgtc
     6421 attgttcagc gtttcgatat ccagcgcgtt cagccccatc attgacgttg ccagcgcact
     6481 acgcagggtg ttctcacgtt cgggcgtcat caccgcctgc aacaaccaaa gcatttcctg
     6541 cgcttccaga gtttcaaaaa cactgtcgcg gttcgaaagg taaacggaag ggatttccag
     6601 caacgttaag gcgtcgcgca cctgggcggc ctcctggcgg ctgcgcacca gcacactgat
     6661 gtccgaagca cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg
     6721 tccggcttgt agccagtcgc ggatttgcgc agcacatacc tgcgccatgg tactttgata
     6781 gtcgccaacg ccgcagcttt cgccttccat cagccacatt ttcatcgcag gctgcgtttc
     6841 acctttaaac acaaaacgta acgcctgatt tttcccggct gatttcactg gaataaacgg
     6901 tatttcgcga aacatgaacg cgtcatcagt ctggctgaaa agcttgttca cactgttcac
     6961 cattcctggt gcggaacgcc agttggtgtc taaagtgtag tgggcgtgaa cttcgctacg
     7021 cgccttcata taagtgaaga tatccgcacc ccggaatgca tatatggcct gtttcgggtc
     7081 gccaatcagc aacaatgcgg tttccggctg atggtgccag atacggcgaa aaattcggta
     7141 ctgctggggg tcggtatcct gaaattcatc aatcattgcc accgggaatc gcgtacggat
     7201 cgccgctgcc aacacctcgc cgctttcgct acgcagcgcg gaatcgagcc gacttaacat
     7261 gtcatcaaaa cccaattcgc cacggcggcg tttttcacgc gctactgttt cgcggatctc
     7321 agccaatgcg cgggttatca ccagatcacg gatcgacaat ggttctgcaa gcagttgttc
     7381 gatcgcctca aacagtggat gtcgcggggt ttcccccccg gctttcgtgc gatcttctaa
     7441 gaaacgctga gagaattttt ccagcgactc cggtaactga taactgttgg tctcttcttc
     7501 tgcccaggcg ctgatcttct cgatccattt agcctgattg ctacggttaa acttgcgtcg
     7561 atcaatacca gaagattcga tcagcgcatc cagttcaccc actgcgtcgc gccactgctg
     7621 ttttaccgta tcaatacgcg ccacaatttg cgcgtggcgg gaagccagcg tttcatcatc
     7681 gggcggcggt gctttgataa ccggtgcttc gccttgcaga taacgattaa tatcgcgcag
     7741 caacgcctgc ggccctttcc aggtttcaaa gaccaccagg gcaatttcac gcggcagcgg
     7801 gtagcagtgg cgacgccaga aatcggcgca ggcctggtag cgtagcagag actcatcttc
     7861 aatcagctgc tgctcaaaca gcataccgga ttcaaaggca ttcaggttga gcatgcgctg
     7921 gcaaaaaccg tgaatagtaa agactgccgc ttcatccatc tgccgttcgg ctaacaacaa
     7981 ccactgcgcg gcttgcgctt tatcgtcgat ctcttccagc aggcgttcgt acagtggatt
     8041 gtcggtggtt tcacgcagac aggcgatgcg caactcgtgg atattgctac ggatacgacc
     8101 gcgcaattct gccgtggcag cctcggtaaa ggtgaccacc agcagttctt caacggtcag
     8161 cgggcgggga aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat agagcgccgc
     8221 aatcgtaaag gttttgcctg tgccggcaga ggcttcaatc aggcgctcgc cctgtaaggg
     8281 caagcgcaaa ggatctagtg tctcggcgac atcactcatt cttttcactc atcaggggca
     8341 ttgtttgctg caacgcgctg acgttctccc acactttcca gccttcagga tgcacatatt
     8401 cggctttccc gttctggctg ccggaaatct gcgacaggat agccatgcct tgcggctcga
     8461 ccaccgcctg atggaagaaa tcagcaagtt tttgcggcgt cagcagtttt atctgggcca
     8521 cgattttatc acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta cttaacttcg
     8581 atgcttcttc gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc gcctgctgga
     8641 tttgcgcaaa ctcatctggc ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg
     8701 ccttgtaacg ctcccacaag aatgaaggct gtttatcatt gctttgcaaa aggaagccca
     8761 tgccccactg acgccccacg ctcattggaa acgcaaacac ggcatagccc aattgttctt
     8821 cggtacgcaa ctgattgtag aaccacggct gtacgatctg ccccaacaga gagctatagg
     8881 ctgagctggt gtattcatcg tagccagtcg gtacaaatac cgctgccagt gcggagtcgg
     8941 tgctgttacc ggctttttca aagatgacgg attgtttttt atcgaccact acatctttgt
     9001 ttcgacacca ctctgaacca tcagcgccca actgtttttg cacatcgcgt gccagcgttg
     9061 ttgcctgggc ctcggtcatg ttgccgataa ccataaactc tggtcgagcc cctgatttta
     9121 aggcgtcgcg ataggccagc acctctttca acgtaatgga gggcaaaatt ttacgccgtt
     9181 catctcgcga gaagtacggc acttgcgaga gcatctgcgc gggcataatc gcctgctcaa
     9241 acgctttacc cttttctgcg gaatccatca tctggttata ccaggacttc gcctgctcaa
     9301 gctgatcttc cgtagcggta tagctaaagt acccctcgag caatgcctgg aacagctgcg
     9361 gcagacgctg ggtgtaacca ttagcattaa ccataaggcc gttgttagcg ttggtggaaa
     9421 aacttatgcc accaaccgac gcctggttgc ttaactgatc aagcgccagc cctgcgagat
     9481 aatcattgag cgcaaacatc acctgattgc gggcgctgtc catggctttc ggattacgca
     9541 aaatcaggct gacatcagct ttgggttcgc tggaaaaata acggcttggc gcatacacca
     9601 cgcgcagatt cgactcatca acaatcagct ctggatggtc atatttcttc tctgacttaa
     9661 tcagcgagaa atcgtcagga atataggggt taagctccgg taaggagagc gcaatattgg
     9721 cagctttttg ctgccagtca gcgaaagttt gttcgctgat tttatcgacc tgatacggcg
     9781 catcgacaaa ataagccgtt ttattgtgcg gctctttcgg gctgatatac cagatacgcg
     9841 cattctgcgg cgtcatcatc gccagacgtt cttttactgc tttagcatcg taccgatcgg
     9901 caatattgac tgcatccagc gtatgctcaa ccggaacgcg aatcatggta tctgccagcc
     9961 attcgacgta atccatatca cgggtgatcg acggataacg gaagtcgata tccagcacat
    10021 tcgccagttc atcgaagtat tgtttatcaa tgcctttttc acgtaacaga ttgagatagc
    10081 taaaaattgc cgccacaacc tgatcgcgat tagccaggcc tttatcggtt aaagacgcag
    10141 agatcgctaa tacgccgctg ttgccgttga cgataggatc ggagttggcg ctaatgccct
    10201 caactaatcc ctgcttttgc agccagtcgg aaagtgtacc tgggctgcga ttaccaatca
    10261 gataggtaat caattcatcc gttttactac ggaacttcgc tgagttgtta tcgatgcgaa
    10321 actcaacgcg cagaacttta cgcggcagtg ccgggacgta atgaatgata atgccctttt
    10381 gcgcgtcggt gactaccggc acggtgattt ccggtttttt gctctctttg ttcggcacgc
    10441 gaccaaaggt gtccgccgcc atttttgcca actccggcag cggtttattg ctgtaaataa
    10501 ccgccttcat cagattggcg gaatagtact tctcgtggaa atctttcagc gcctgctgca
    10561 ccggattacc aggtttatcg cttaaagttt cgaggttacc accagaaaac tttgaaccgg
    10621 ggtgtgccgg gttaatggtt tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac
    10681 gcgccatggt taattcagcg ttcaccgcat tacgctcacg ttcggcatat ttcttgtcga
    10741 gcaaaggttc agcaatagca tcggccaggc ggtctaccgc accaggcaag gcgtcgttct
    10801 caacttccag atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt
    10861 gcattttgag atattcggcc agactgtcag cctgcgggta ctttttcgac cccatcagac
    10921 tcatatgttc aaggtaatgt gccagcccct ggtacgcctc gggatcttcc agcgacccaa
    10981 cgggcaccac cagcgccgag agcgatttaa ctgcctgcgg atcagaaacc agcaagacca
    11041 ccataccgtt atccagacgt atagcctgat actggcggtt atctttatca cttttacgga
    11101 tggtttcctg aatcggctgc catcccgttt ctgcctgact taagggtgcc caaagggcaa
    11161 caaacaacaa taatgctttg aaccaggtgc tgcggggcat tcacggacct cataatcaac
    11221 tttattttct ggccagattc aacaggttaa cgcaaatcat ctgccagaat ttaatcttgt
    11281 gctgcacgag tcagcctatg tctatatatc catcagtccg tgactggtgc gcatcataat
    11341 gtaagcgaat gaattgcgca atttttatac agcactcatg actgattaaa gcgaaacagc
    11401 ggtaacagga aacgttgcga ctgttcaacg atggcctcca ttgtctctgg tgttaattgc
    11461 cgccagagcc gttgatacca gatatcatca ccttcgccac gcaccatcat gttgccttcg
    11521 taagcctgaa ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc cagcatggca
    11581 tcgttttgcg cgtcataaca ggtttttagc cacgcgccgc cactttcagg taacaccagc
    11641 aatggcgcgg acattccttc acgatacccc tcaatcagtt gtgagaggta atgcaaagcc
    11701 tgttcggctg caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt
    11761 tcaccattac caccgctggc acagtagaca aggtgttcca gccaaagttg cattccctgg
    11821 gccacactta ataaagaggg acgccagcgc aacaggccat ccggctgcac ctgcggcaac
    11881 cagccagtta tctgcacacc gttgcaggcg agatcaattt ccatactctg ccccggctgg
    11941 cgacaggcaa tgactctgtc ggcaagctgc tgcatctcct ggcactgtgt ttcccagaaa
    12001 atttcaccaa aagcgccata cggtaaatcc cctgccgccc ggaagcggcg gaacaagcgt
    12061 tcggcatcat cctgctcaac cagtgcattc aataactgct gattgatttg ataacggcta
    12121 agcccttcca gaataaatgg ctcggtgtcg gggatttcgc tgtcttcggt acggaagttc
    12181 acctgcaaac gcatctggaa aaatgcccgc accggatgtg cccagaatcg ttgtagcgtt
    12241 tccagcggca cggtttccgg taaggtaaac ggcagcggct gaacaaattc agaatgtgct
    12301 ttaccagcct ggctggccgc aggtagccat tcacgagcat aactttgtcg ttcgcctggc
    12361 tggtagtttt gcggatcaaa cggcattcgg gtatgaaggc aagtaagatg cgattttacc
    12421 cttgcctcgc tttcatcaca gttgagcgcc tcatcgcccg gtagataatg actttgtccg
    12481 atgtagtcga tcagttcctg caccagtacc gacgggaaac gttcactgtt atcctgaatg
    12541 gaacgcccga tatagctgat atagagtttt tgctgcgcgg aaattaacgc ttccaggaac
    12601 agatagcggt cgtcatcgcg acggctacgg tcgccacgct tcggtttctg gctcatcagg
    12661 tcaaagccca atggcgcaag ctggcgtgga taaacgccgt cgttcattcc cagcaggcaa
    12721 accactttga atgggatgga acgcattggc atcagagtac aaatgttaac cggtccggcg
    12781 agaaaacgct gactgatacg ttcctgatcc aggcgctgtg ccagttcatc acgcaatagt
    12841 gacagcggca ccgcgtcgcc atactgcgca cctaaacctt cggcgataat cgcctgccat
    12901 tgttgttcga tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa gaaggcgttg
    12961 agcatatcgc gacaaaccgg caaccactct tccagcggac gctcctgcgc cagcccgcga
    13021 cgccagatgt ttagctgcat tagcagtgaa gccagatgcc ccaccagttc tgcaattaag
    13081 ccgctcgatt catcataagg tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg
    13141 tagcccagca acatacgcgt caggccaaac cgccaggtgt gttgaccggt ggcggggagt
    13201 tccagctcgc gaacgttatc gtcatctatc ccccagcgaa tgccggattc gttgacccac
    13261 tggcgtaaat aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag caccggcaca
    13321 tccagcagcg ccagcacatc ctctgacaca aagcgactgt caggcagtga taacaggctg
    13381 ataaacgctt ccagtaccgg atgtgactgc cgcgcccgac ggtcggaaat ggcgtaaggc
    13441 aggtaacgat ccgcaggtgc gctaccaaac acagcctgaa taaacggact gtagctgtcg
    13501 atgtcagcca ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc ctccagcatc
    13561 gccagcaagc gatcgtgtaa aacttcaact tcacgctgcg ggctatggca aacgtggaag
    13621 gtgatactgc tatccagtgg atcaagcggg cgtttgttat cgctacggga aaactcttcg
    13681 atgttcacac ccgcaacggc gcggttttcc agttccagaa tgtcagactg aatgttatgc
    13741 agcaggttgt ctggcgtcac atcgacaaaa gcgtccagct cctggctgct ctccaggtca
    13801 gaaaggagat aaatgtagtc gcgcccaagc ttcccccatg aggccagcag cgggttgccg
    13861 acatcctgtt caccatcgct gttaaagagc tgtccggcat tttcgctatc gcgaaatagc
    13921 ggcaattcgc gatcttcaaa actgtgtcgg cgctggcgag tcagtagttt cgccagataa
    13981 gcaggatctt taatgtcgcc ccagtaataa cggcaggggt tggtaaacag gagatggatt
    14041 tcaatatgtt tacccagcgc ctgtagcgcc tggagataaa caggcggtaa cgcggaaata
    14101 ccgcatataa agacgcgcga aggtagcccc ggcgggcagg tcgtcgcgga ctccagcgtt
    14161 tcgataaagc gctgatagag attggcgcgg tgccagcgcg gttgcccgag ttgatgggta
    14221 tattccacca acgccttcca caacggcgct tgccaggcct gtgcttctcc cagcccttca
    14281 accaaatgtc ctgtttccca ctgtgccagc cagtccggac gatagaccag atactggtca
    14341 aacaggtccg ccgcttttga agaaagctgg aacagttttc gcttgtcgct atcgtcagtc
    14401 agataatgcc gcaacagggt aaagtcttcg cgctccaaca attgcggcag cagagtcatc
    14461 agtttccagc tcatgctctg tttgttaaag gcgctctctt tggggatttc cggtaacacc
    14521 cggacgaaca tatcccagat aaagctcgct ggcagcggaa aatcaatgtt tgccgcaata
    14581 ccaaactttt gcgacagggt catttgcagc cactgcgcca taccggtact ttgcaccaga
    14641 atcatctctg gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac
    14701 gcttccagca cgtccagacg attggaatgg tagaccctta acatagcggc tcctgactac
    14761 tgacgattcg ggcaatgcag gcgggtcatc tcgccttctc tgcccccggg tgaaactagc
    14821 gtaacgctga tgctgacaca tcccgcctgc gatgtctgca ttcggttgac ctgccagttg
    14881 gcaggtggcg aaatcgcgcg cagttgcgtt tgctgccaac catgccgcca gagctgttgg
    14941 tactggtttc tgctggcgag actgttcatt aatgttcgct ggatacccga taacgcagtg
    15001 acaatcatca ccatcagcac catcgcaacc attacctccg gcaggctaaa gccttgttga
    15061 ttcttcaggg aagctgacat aacgccctct ctttcagcgg acaaaaatcg ctccagccgc
    15121 gtggcgaaaa aacaatgttt ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt
    15181 cataaccggc aatcaataag gcttcattat ctgccagtaa acgcaaacaa acctgggcat
    15241 cggttccagc gtactgcgag cactgaactg ctggctgcgt ctgccaggag tgcatttttc
    15301 cccacgccag cgccgactga acgatggcct ggcggcgcaa tgactgactt tccatgctca
    15361 cgcgagaggc aaaactgcga tcctgctgac tcattccttg caatagcaag ctacccaaaa
    15421 ccagcagcat caggaccaga gccagtgacg aaacaccttt ttcgcggttc acaggttgaa
    15481 tcctgtcacg ctatagctgg catccaccac ggtttgcggt tcagacttac tggcagcacg
    15541 catattaacc gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt
    15601 gtcgataatg atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt
    15661 cgcaccgcgt agcgtttcca gcacatgctc cttcagacga aatccaatct ggtcagactc
    15721 ttttaccggt tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca
    15781 gtcaccctgt ccgacaattt ccagcccttc gccggtacag ctgccatgac aataacccgc
    15841 cctctggaga tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagcgccag
    15901 cttacgggtg ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg cagcccccag
    15961 caacaatacg ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc
    16021 ttttacaggc atccttctgt ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa
    16081 accaccagcc accactcgcc cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta
    16141 ttgcgcaggc caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag
    16201 cgtggcacaa agaccaatgg tgaactgcca tgacaggtat tggccccagc agcggaactc
    16261 acaaggcacc ataacgtccc ctccctgata acgctgatac tgtggtcgcg gttatgccag
    16321 ttggcatctt cacgtaaata gagcaaatag tcccgcgcct ggctggcggt ttgccatagc
    16381 cgttgcgact gctgccagta ttgccagcca tagagtccac ttgcgcttag catgaccaaa
    16441 atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca
    16501 gtatggagcg aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg
    16561 gactatttac gccgttgcag gacgttgcaa aatttcggga aggcgtctcg aagaatttaa
    16621 cggagggtaa aaaaaaccga cgcacactgg cgtcggctct ggcaggatgt ttcgtaatta
    16681 gatagccacc ggcgctttaa tgcccggatg tggatcgtag ccttcaatct caaagtcttc
    16741 gaaacggtag tcgaagatgg attcgggttt acgtttgata atcaacttcg gcagcggacg
    16801 cggttcgcgg cttaattgca gatgagtttg atccatatga ttgctgtaca ggtgcgtgtc
    16861 gccccccgtc cagacaaaat cgcccacttc cagatcacac tgctgcgcca tcatatgcac
    16921 cagtaacgcg tagctggcaa tgttaaacgg caggccgagg aaaacgtcac aggagcgctg
    16981 ataaagctgg caagagagtt tgccgtctgc cacatagaac tggaagaacg catggcacgg
    17041 tgccagcgcc attttatcca gttcgcctac gttccacgct gaaacaataa tgcggcgcga
    17101 atccgggtcg tttttcagct ggttcagtac cgtagtgatc tggtcaatat gacgaccatc
    17161 cggcgttggc caggcgcgcc actgtttacc atacactggc ccgaggtcgc cgttttcatc
    17221 ggcccattcg tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc
    17281 ctgcagaaac cacagcagtt catggatgat ggaacgcagg tggcaacgtt tagttgtcac
    17341 cagcgggaat ccatcctgca ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc
    17401 ggttccggta cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa
    17461 atactgtttc atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac
    17521 catcatgatc acacccgcga caatcatcgg gatggaaaga atttgtccca tgctgatgta
    17581 ctgcacccag gcaccagtaa actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg
    17641 aaacgcgccg taaccaatca ggaacaaacc tgagacagct cccattgggc gcggtttacg
    17701 aatatacagg ttgaggataa taaacagcac tacaccttcc agcaacagct cgtaaagctg
    17761 tgatgggtgg cgcggcagca caccgtaagt atcgaaaatg gattgccact gcgggttggt
    17821 ttgcagcagc aaaatatctt ctgtacggga gccagggaac agcatggcaa acgggaagtt
    17881 cgggtcaacg cggccccaca attcaccgtt aataaagttg cccagacgcc cggcaccaag
    17941 accaaacgga atgagtggtg cgataaaatc agagacctgg aagaaggaac gtttagtacg
    18001 gcgggcgaag ataatcatca ccacgataac gccaatcagg ccgccgtgga aagacatgcc
    18061 gccgtcccag acacggaaca gatacagcgg atcggccata aactgcggga aattgtagaa
    18121 cagaacataa ccaatacgtc caccgaggaa gacgccgagg aagcccgcat agagtaagtt
    18181 ttcaacttca tttttggtcc agccactgcc cggacgattc gcccgtcgtg ttgccagcca
    18241 cattgcaaaa atgaaaccca ccagatacat caggccgtac cagtgaagcg ccacgggtcc
    18301 tattgagaaa atgaccggat caaactccgg aaaatgcaga tagctactgg tcatctgtca
    18361 ccacaagttc ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt
    18421 caggcgctcc aaaggtgcga ataatagcac aaggggacct ggctggttgc cggataccgt
    18481 taaaagatat gtatatgatc cgcgctataa ccctccgcga atcagcccgc ccatgccgcg
    18541 acgctccata aaggctgcaa cctgatggcg aacttcggtc gccagttgcg cttccagact
    18601 acgctgcgca agattttctg cgtcggcata atcaatgcgc cgcagcaggt attttacccg
    18661 cgctacagaa cgtccgttca tagacagatg gcgataccca agcccaatga ggattgccac
    18721 gcacatggga tcgcccgcca tttcaccgca caaacggaga tcgattccat gtatttccgc
    18781 ttcccgggcg atcatcgcca gagctcgtaa cattgcagga tgaagactgt cataaatgtt
    18841 cgccacccgg gtattgttgc gatcaacggc cagaatgtat tgagtcagat cgttggtgcc
    18901 aacagagatg aaatcgaccc gctttgccag atgcggcagc ataaatacca ttgacggcac
    18961 ttccagcatg atgccgatac gtggtttggg aatttcgtaa ccgatcatct cctcgacttc
    19021 acgtccggca cgttcaatca ggcggcgtgc ttcatcaact tcatcgaggc ttgtgaccat
    19081 cggcaacaga atattcaggt tgcccgtagc ggcattagca cgcagcatcg cccgcacctg
    19141 gatcaagaag atctccggct gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg
    19201 attctcttcg ctgatcggca tgtaaggcag ctgcttatct gctccgacat ccagcgtacg
    19261 caaggtgacg ggtttatcat tgaacatttg cagcatcccc tgatactgcg ccacctgttc
    19321 ttcttccgac gggaaaccac tttgcagcat gaatgggatt tcagtgcgat aaagaccgat
    19381 gccatcaata cggctgccca gtttttcttc atgttccggg cttaaaccag cattgagcat
    19441 gacttttata cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat cttccgccag
    19501 acggctaagc tcaatctctt cactaattag ccgctgatat tcttgcagca gtaccggctc
    19561 cggatcgacc agcaattcac cgcgataacc atcgacgatc agcgtccgac gatgcagcac
    19621 cgaaggctga atatccgcgc ccatcacggt agggatcccc agcgcacgta ccatgattgc
    19681 agcatgggag ttggctgcgc catcgcgcac gacaacaccc actaagcgat cctggggcag
    19741 ctcagcaaga gttgtcgctg acagttcatc tgccaccaga atgaaacgtt ccggccaggc
    19801 gttcggccct tgattagcgt catcaagatg aaacagcaat cgctgaccca gcgcacgtaa
    19861 atcgccagcc cgctctttga gatagttatc gcttagcgcg gcaaactgtt cggcaaattt
    19921 ttcaatgacc gtttttaccg cccactctgc caccgagcct ttatcaacct cggcaaacaa
    19981 ttcgcgacgc agccgggtgt ccgaaagcag gtgcgagtaa agatcgaaaa tagccgccgt
    20041 ttctttttgt gctccggcgg caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc
    20101 ttccagcgcc ccggtcagtc gttcgcgttc cagagccgga tccagcgttg atgcctgata
    20161 cacctgttcc attaaaggta acgtggcatc ctgccagcct tcggcaatcg ccacaccagg
    20221 tgctgccggt aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaggg cagtcaactg
    20281 cgactgagaa agaatagctg ccatctgggt ggcaagcgtc accaggaagg attcttcact
    20341 ttcgtcatac tggcgcaact ctcgttgctg taccaccagt acaccaagca actggcgacg
    20401 ttgaataatt ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg aggggatgta
    20461 tttgaagctg gggtgctttt gcgcatctgc aaggtttatc ggttccgcca gcctgccaac
    20521 caggccgacg atcccttcat caaacgcgag cgttacagtg cgaccgcgtg gttttttcag
    20581 cccccgggtc gccatcaggt agtaacaacg tcgatcatga tcggccaggt agaccgaaca
    20641 gacctcggta tccatcgcaa gacagatgtc ggtaaccaga atatttaacg cctcattcag
    20701 gcgtggtgcg ctggctacct tttcgactat ttcgcgcagg cgagtgagca taattggcgt
    20761 gacttaacct cttttacgtc gataagcaga tgcgttttgt ggttttggcg tattttcctg
    20821 cagtgacatc accacactcg cgaactcttt cattacccta cggtagacat cacgtttaaa
    20881 tgacaccacc tgtctgaccg gataccagta acttacccat cgccagccgt caaactctgg
    20941 tgtactgctg gtttgcatat tgatttctgc atcgccgctc accagctgca agagaaacca
    21001 tttttgtttt tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa
    21061 tttgtagcgc aaccagttac gcgttgaagc aaggattcga acgtctttgc ggcttaatcc
    21121 tacttcttca aacaattcac ggtacatcgc ctgctctgcg gattctccgg ggttgattcc
    21181 gccttgcgga aattgccagg agtgctgacc aaatcgccgg gcccacatta cctgcccctg
    21241 gcgattacaa atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact
    21301 acctcaaaat aaagctttat atacgaatga ttgtttcata ctccaggaag acggtaaacc
    21361 actctctgca gggcattaca cactaataac aattgaataa ctcacagtta tgtgcagagt
    21421 tataaacaga ggaaggggtg gatagccgtt tttattcacc ttttctgtgg atagagttgt
    21481 gaagaactac ggaattacta cgggaaaacc cggagcattc tgaataagcc cgatatagac
    21541 attttaaaat attcattatt agtcaatgca ttacatgtaa tttgagtaat gaaaaagagt
    21601 aattcgtgac ccaggtcaca cctctcattt acgggttggc gaaagatcaa ccaatgccgt
    21661 atttatccac agaatgtgcc actaagttaa gcactgaacc actaaaaact ggagtttcgt
    21721 cgcacgtcaa ggctgtaaat ggaaacagta gtggaggttt ttcacagtta tcccagcttt
    21781 ctgtggataa catggtgtaa gatcctgttt attttcagtg accagatttg gaaaacccgt
    21841 tgcagtgttg cgcaactcga ttaccggcag cctaaaaagc aatataaatc agggaattga
    21901 acaacacatg tggaaaaagt tacactgcga atattcggca cataattgct gtttgttttt
    21961 taatcaaggt atcatgacat gtcccaacct cgcccactgc tctctcctcc cgaaactgaa
    22021 gaacagttgt tagcgcaagc acagcaactt tctggttata cattgggaga actggcggca
    22081 cttgccgggc ttgttacgcc agagaattta aaacgcgata agggctggat tggcgtgtta
    22141 ctggagatct ggctaggtgc cagcgcaggg agtaaacctg agcaagattt tgccgctctg
    22201 ggcgtggaac ttaaaactat ccctgtggat agtcttggtc gtccgctgga aacaacattc
    22261 gtttgtgttg ccccgttaac gggtaatagc ggggtgacct gggaaaccag ccatgtgcgc
    22321 cacaagctca aacgcgtact gtggataccg gttgaaggcg agcgcagcat cccgctggcg
    22381 cagcgtcgcg taggatcacc gttgctgtgg agcccgaatg aagaggaaga ccgacagcta
    22441 cgcgaagact gggaagaatt aatggatatg attgttctcg gtcaggttga gcggatcacc
    22501 gctcgtcacg gggaatattt acagataaga ccgaaagcag cgaatgcgaa agcgcttacc
    22561 gaagccattg gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt ttatttgaag
    22621 aagaatttca ccagtgcact actggcccgt cattttctga tccagtagcc atcgctttga
    22681 cctgccgctt tccgggcata taattaccgc ttcatttttt tggcagggct ttttagatgt
    22741 tatttgcatg gataaccgat cctaacgcct ggcttgcgct cggtacgctg acgctgctgg
    22801 agatcgttct tgggatcgac aatattattt tcctttctct ggtggtggca aagcttccca
    22861 cagcacaacg tgctcatgcg cgccgtctgg ggttggcggg agccatggtt atgcgtctgg
    22921 cgctgctggc atcaatcgcc tgggttacgc gcctgacaaa tccgcttttt acaatattca
    22981 gtcaggaaat ttctgcccgt gatttgattc tgcttctggg tggcttgttc cttatctgga
    23041 aagccagcaa ggaaatccac gaatccattg aaggtgaaga agaagggctg aaaacacgcg
    23101 tttcatcatt cctcggcgct atcgtgcaga ttatgctgct ggatattatc tttagcctcg
    23161 actcggtgat tacggctgtg ggtctgtcag atcacctgtt tattatgatg gcagccgtgg
    23221 tgattgccgt aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt gtcgaacgcc
    23281 atccttcggt aaaaatgctg gcgctctctt tcctgattct ggtgggcttt accctgattc
    23341 tggaaagttt cgacatccac gtaccgaaag gttacatcta cttcgcgatg ttcttctcta
    23401 ttgcggttga aagcctcaac ttgattcgca acaaaaagaa tccgctctga tactccgtac
    23461 gctctcctgc gggagggcgt actcactcct tttaccctca cagtttaaga ttttcctgct
    23521 ttcaagatat ataacgtcgg tttataaaca gactattatc ataggtaggc caaatgagga
    23581 taaccggatg aaaaaatggg cagtaataat ttctgcagtc ggactggcgt ttgctgtttc
    23641 cgggtgttcc agtgattacg tcatggcgac caaagatggc cgtatgattt tgaccgatgg
    23701 aaaacctgaa attgatgatg ataccgggct ggtgagttat cacgatcagc aaggtaacgc
    23761 gatgcaaatt aaccgtgatg atgtttcgca aattattgaa cgttaacaaa taaggtcagc
    23821 atccggctgg ccttaagatt tttctctttc ccttttccct tccctctgcc atttttatat
    23881 tccttatgtc gtgattataa aaaggaaacg gctatgcaat atcaccgtat accccacagt
    23941 tcgctggaag tcagcacgct ggggcttggc acgatgacgt ttggtgaaca gaacagcgaa
    24001 gccgacgccc acgcacaact cgactatgcc gtcgctcagg gcattaacct tatcgacgtt
    24061 gccgaaatgt acccagtacc tccgcgcccc gaaacgcaag ggttaaccga aacctacgtc
    24121 ggcaactggc tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc caaagtgagc
    24181 ggaccgtcgc gcaataatga caagggcatc cgcccggatc aggcgctgga tcggaagaat
    24241 atccgcgaag cgctgcatga cagcctcaag cgcctacaga ctgattacct cgatctttat
    24301 caggtgcact ggccgcagcg cccgaccaac tgcttcggca aactcggtta tagctggacg
    24361 gattctgcgc ctgcggtttc gctgctggat acgctggacg cactggcaga gtaccaacgc
    24421 gcgggaaaaa ttcgttatat cggcgtgtcg aacgaaactg catttggcgt aatgcgctac
    24481 ctgcatctgg cggacaaaca cgatctgccg cgtattgtca ccattcagaa cccttacagt
    24541 ctgttaaacc gcagttttga agtaggtctg gcagaagtca gccagtatga aggggtcgaa
    24601 ctgctggcct attcgtgcct gggtttcggc acgctgaccg ggaaatatct caatggtgca
    24661 aaacccgctg gcgcacgtaa tacgctcttt agtcggttca cccgctatag cggtgagcaa
    24721 acgcaaaaag ccgtcgcggc gtatgttgat atcgccagac gtcatggcct ggaccctgct
    24781 cagatggcgc tcgcgtttgt acgccgtcaa ccgtttgttg ccagcactct gctgggcgca
    24841 accacgatgg atcagctgaa aactaacatc gaaagtttgc atctggagtt aagcgaagac
    24901 gtattagctg aaattgaagc ggtgcatcag gtttatactt atccggcacc ataagattaa
    24961 cgccggttaa aaccggcgtt aaatattaat gacggcgctg ccagatccac agcgccgtta
    25021 ttgccagcgc aaacagcgca ccgaagccaa tgccaatggg cacgaccggg atgcctatca
    25081 ttaccgccag cgagtaaatg cccagcatca acaacatggc gctgttttcg ccaaggtttt
    25141 gtactgcaat cgcattcccc gccccgacgc tttttttacc ccgctcctgt agcaacgcat
    25201 tgagcggaac gacaaaaaag ccccccatca cgccaatcag catcaacaag gcataggctg
    25261 gcagcagctc gtgttgcagg gaaaaaatca gtaccaccac gccaatcaaa atcccggctg
    25321 gcatacagcg tgacacggtt tccagcgtaa ctaacttcgc tgccgcacct gcgccaacca
    25381 cgataccaat cgctaccatc gcgttgagat aggtgggcgt agcgttatcg gtaatgccca
    25441 gcgccaccgg tacccacagc accaacagga aacgcagcgt gacacccgct ccccagaata
    25501 aactggtgcc caccagcgaa aaacgcgttt caccattgcg ccatagcgag gtgcaggcat
    25561 tcaggaaact gcgggtcatg ttgatgagat tccaggactg ccccggacgc gccgccgcca
    25621 gtttgggaat gtagatattg gcaacgaccg caccaccgta ggccagtgcg catgcggcca
    25681 gggcgacgag gacatgccag tcagccagca caccaccggc tacggaaccg agcaaaatcg
    25741 ccgctatggt agaagcttcc attaaaccgt tagctttcac taacttacta cccgtggtta
    25801 attcgccgag aataccgtat ttcgccggtg aataggctgc agcaccaaca cccaccagcg
    25861 tatagccgag aaacggattg ataccaaagc agatactggc tgcgcccagc agcttcaggc
    25921 cgttggcaaa catcatcacc cggcctttgg cgaagctatc cgccacctgc ccgacaaacg
    25981 gcgcaaaaag aatgtaagca cctacaaaca ccatttgcag gatgggctgg ctccactccg
    26041 gatagaactg cgctttcagt aacgccagag tggcaaacag tagggcatta tcgccaaacg
    26101 cagagagaaa ctgcgccacg ataaccgctt tcatcccctt cgaccacaac gaagtgttag
    26161 tgtgcactga ctcactcatc gtgttgttcc gcttcgtcta cccagctttt caacgtgaca
    26221 aagtcaggtt tgccgctgcc aagtaatggc atctgtttca gatagcgaat atcgcgcggt
    26281 acagcaagct ccggcacgcc gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt
    26341 tcgttatctg tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct cttaatcgca
    26401 gtggcatgga ctttatctgg cgaaacacca agtgccagtt gttccaccat ttccagcgac
    26461 accatttcgc ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc
    26521 tgctcgtcaa aacgcacaat atcgccagtg tcataccagc cgcgctccat ttcgccgcga
    26581 acattctcgg cggtgggcac ttccagtaca cctggcttct ccacccgcag atagccgttc
    26641 attatgttcg gccctttcag ttgcaggcgt ccgccctctt cgataccagg aaccgacaac
    26701 aggcgcgcat ccattcctgg tagaatacgc cctaccgtac cgggtttcgc cgccatcggt
    26761 acgttgatag aaacgacagg cgcgcactcg gttacaccat agccttcaag gatgcgcagg
    26821 ccaaatttat cctgccaaag ctgtttggta ctttcttgta gtttttctgc gcctgccacc
    26881 acatagcgta gacgatagaa gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa
    26941 gtcgaggtac cgaacaacac ggtgcaactg cggtcataca ccaactccgg cacaatgcgg
    27001 taatgcagcg ggcttggata aaggaacact tctgcacctg taagcagtgg cgtaaacagg
    27061 cctaccgtca gcccaaagga gtgaaacagc ggtaacgccg acataaagcg atcgttggtg
    27121 gtgaagtcgg caatcgtttt aatctgctcg acattcgcca gaatgctttt atggctatgg
    27181 acgacgcctt tcggatggcc ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc
    27241 ggctgctgtt taacctgtgc cagacgcggc atcagcaaat gagcgaagat ccatactttg
    27301 tcggcagtgg tgacatctgc ttttaaatct tccagataga cccagcgcac ctgagtaagt
    27361 tgctccggca gatgccagag tttgccttta tcgagaaact ggcgggaagt gaagatggtt
    27421 ttgatttcag ccgccgtaat agcactggtc agccctttta ccccggcagt gtagttcatc
    27481 attgcgggca tgcggcgacg ggcgatggcc ccaaaaatca ctgccgcact gatgcctgca
    27541 ttgggcagca ttaagccgat gcgttcgcct tcaacactgt atttttcaag gatgcgtcca
    27601 acaaacagcg ttttcgtaag caatttgcga taggagtctg gggtaaagtt gacgtcttcg
    27661 acacatttct tcccggctcc gaagcggtac attgcactca gtaaagattc gtacagcgtt
    27721 tcacgcgggc gcaccgccat tcgcgcttcc atcattattt gatgcagcat ttcgccagcg
    27781 attttgcgac ggtcacgggc acgcggcgca tccggcatcg ccacctgcgt tggtggcaaa
    27841 atatgcagag taatttgcgg gaacaagcga cgtttaacca gacctttcag gcggctgaag
    27901 tgcgtaagtt ccgccccttc aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg
    27961 acaaaacccg cgccatcgta gattttcatc agcgagcctg tagtggtgat gcgtccttca
    28021 gggaaaatca ccactggtcg gccctgttcc accagacgta ccagatgttt aatagccata
    28081 ggttgcgtcg ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg catataccac
    28141 tgttggctta ttgaggtgta aacggcaaac actggacgca caggtaaaaa cagtccaagc
    28201 aaaatgccat caataaaaga gacgtgatta ggcgtaatta gaacgcgctc gcccttcagt
    28261 gcctgggtgt cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag
    28321 ctaaaaagca ttccaactcc ctttgctctg attcagtaaa agcgaatgga gggagattac
    28381 acgagataaa gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg
    28441 caggttggtg caagagacag ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc
    28501 tctgggatct tgattgtggt ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa
    28561 tgaagggaaa tgcaacgagg tgtgtaaatt gtcggttact gttacagatt gatgaccggc
    28621 aaaaaaaaac ctgcgcatct gcgcaggctg gtgtaattca tgtgctcaac ccgaagttga
    28681 cttcacctat caatacctct gggatcacca ctttagcaac ctgaagccaa acgccaccag
    28741 cggtcaatcg caacagcgtt tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt
    28801 cttacttatt tgcgtttggc tcacattccc acgatgaaaa cacgccaccc cttgaaccaa
    28861 cgggcgtttt ccgtaacact gaaagaatgt aagcgtttac ccactaaggt attttcatgg
    28921 cgaccataaa ggatgtagcc cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca
    28981 ttaataattc acctaaagcc agtgaagctt cccggctggc tgtgcatagt gcaatggagt
    29041 ctcttagcta tcacccgaac gccaacgccc gtgcgctggc gcagcagacc actgaaacgg
    29101 tcggtctgat cgttggtgat gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg
    29161 aacaggtggc ttatcacacc ggtaatttct tattgattgg caacggttac cacaacgaac
    29221 aaaaagagcg tcaggctatt gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc
    29281 atgccaaaat gatcccggat gccgatttag cctcattaat gaaacaaatg cccggtatgg
    29341 tgctgatcaa ccgtatcctg cctggctttg aaaaccgttg tattgctctt gacgatcgtt
    29401 acggtgcctg gctggcaacg cgtcatttaa ttcagcaagg tcatacccgc attggttatc
    29461 tgtgctctaa ccactctatt tctgacgccg aagatcgtct gcaagggtat tacgatgccc
    29521 ttgctgaaag tggtattgcg gccaatgacc ggctggtgac atttggcgaa ccagacgaaa
    29581 gcggcggcga acaggcaatg accgagcttt tgggacgagg aagaaatttc actgcggtag
    29641 cctgttataa cgattcaatg gcggcgggtg cgatgggcgt tctcaatgat aatggtattg
    29701 atgtaccggg tgagatttcg ttaattggct ttgatgatgt gctggtgtca cgctatgtgc
    29761 gtccgcgcct gaccaccgtg cgttacccaa tcgtgacgat ggcgacccag gctgccgaac
    29821 tggctttggc gctggcggat aatcgccctc tcccggaaat cactaatgtc tttagtccga
    29881 cgctggtacg tcgtcattca gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca
    29941 gcgactaacc gcagttaaag caattccagc gccagtaatt cttcgatggt ctggcgacgg
    30001 cgaatcaacc gcgcctgacc attatcaaac agaacttctg gtaacagcgg acggctattg
    30061 tagttggatg acattgatgc gccatatgcc cctgtatcat gcagtaccag ataatcaccc
    30121 gccttcactt ccggcaaggc gcgggtttca acatttcccc cttcctgctg ggtaaagaca
    30181 tcgcccgatt cacataatgg cccggcgacg acggtttcca ccgttggcgc gtgttcctga
    30241 gaacgaccat cagctgccag ggcactgata tggtggtaac taccgtacat tgccgggcgc
    30301 atcagatcgt tgaacccgac atcaaccagc acaaagtggc ggctacccat ttgtttgacg
    30361 ctccgcacct gagtaattaa cacgccagac tgcgctacca gaaagcgacc cggttcaatt
    30421 tccagtttca cagggtggcc caaatggcga gcgatttgct cacgcgcggc attccacaga
    30481 ccataataat gttcggtatc aaccgcctct tcaccctgtt gataaggaat agaaagccca
    30541 ccgcccgcag aaatagcctg taaatcctga ccgaattcga tgacctgacg taccatagca
    30601 ccacacacct gttccagatg ggcataatca acgccagaac caatgtgcat gtgaatgccg
    30661 accagctgca aatgatgacg ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag
    30721 ataccgtgct tgctgttttc gccaccggta ttggtttttt ggctatgtcc gtgaccaaac
    30781 cccgggttaa cgcgcagcca tacccgatgc cctggcgaaa cctggcccag ttggtcgagc
    30841 atatcaacag aacccgcatt caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc
    30901 tgatcgataa catctgccgt aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc
    30961 gccgccaacg cacgctctat ttcgcctaac gagacggaat ccactttcac gccctgctca
    31021 cgcattaagc gcaaaatatg aatattggaa caggctttct gtgcaaagcg caccacatca
    31081 aactgtttca gcgctgcaat ctgccgacga ataatttgcg catcgtagac ccacaccggg
    31141 cagccaaatt cagcgggcaa acgcagcaga ttttcggcgg tgagatcggt atcggtgctg
    31201 aacagtgaat gtggcataac aaactccaga taagtgcttt tttatgatta cgccacatca
    31261 taaaaagaat aaaaaatatc gatttatgtc gagtctatgc aaaaatgata tggattaccg
    31321 gattgcgaga gagcgctaat ggccgccgtt aacttacgtc atattgaaat ttttcatgcg
    31381 gtaatgaccg ccggaagcct gactgaggca gcacacctgc tacacacctc acagccaacc
    31441 gtcagccgcg aactggcgcg ctttgagaag gtgatcgggc taaaattgtt tgagcgcgta
    31501 cgtggacgat tacatcctac cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc
    31561 tggtacggac tggatcgcat tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga
    31621 gaactgtcta ttgcctgcct gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa
    31681 ccctttctgg cacgttatcc cgatgtcagc ttaaatatcg tgccccaaga atcaccgcta
    31741 cttgaagagt ggctctcggc ccagcgtcat gatttaggac tcactgagac actccatacg
    31801 cctgcgggaa cagaacgtac cgaattactc tctttagatg aagtgtgtgt gttacctccg
    31861 ggtcatccgc tggcggtaaa aaaggtatta acgccggatg attttcaggg tgagaactac
    31921 atcagccttt cccgtactga cagctatcgc cagttgctgg atcagctatt tactgaacat
    31981 caggttaaac gacgcatgat cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta
    32041 cgggcggggg taggtgtttc ggtggttaac ccgctcaccg cactggatta tgcggcaagc
    32101 ggtttagtgg tgcggcggtt cagtattgcg gttccgttca ccgtcagcct gatccgcccc
    32161 ctgcaccgcc cgtcatcagc gctggtgcag gcgtttagtg agcatttaca agcggggtta
    32221 ccgaaactgg tcacttctct tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga
    32281 cagcatcctc ggcatggatc gccgcagtat caaacacagg caaaacactg cgctcttctg
    32341 gcaccagtaa accaatttct gtgcagccaa aaatgacgcc ctgtgcgccc tgtgcgccct
    32401 gttctgcaag gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc
    32461 ccagacacag ttcttcaaaa ataatctgat taattttcgc ccgttcatcc gcttcaggaa
    32521 taagacaatt gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg
    32581 tgtaacgcgt acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg
    32641 tggcatccgc aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca tccgccactt
    32701 tatgcatcgt attggtacat agcacaatac cttctgcgcc cgcccgctgt aagccaagcg
    32761 ccgcctcagc cagaatgtcc ccggttttat cccattcccc gcgacgctgg cactcttcta
    32821 tttcatgaaa atcgacgcta tgtagcagca cttgcgcaga gtgaagccca ccaagccgct
    32881 gtttaatgcc ttcatttatc agacgatagt aaggaatggt ggattcccag ctcattcctc
    32941 ccagcaaacc aattgttttc attcctctct cctgatgtgt gttaccgcaa caaggctgaa
    33001 taaggaacga gacaaacgcc tcaacggcca ggtgcccgat ctctattaac gaaaaaaggg
    33061 ccggatgtac agcacatccg gcccatgaaa tcagacgccg atatttctca acttctcgcc
    33121 tgccatcagt ttgcgttcga tatgttccag cgtgacattt ttggtttccg gaatgagcca
    33181 gaaagtgatg cccacaaacg caatgttcag cgcagtgtag agccagaacg taccggcagc
    33241 gccaatgcta tcaagcagtg tcaggaaggt cgcgccgata atcatattcg acacccagtt
    33301 tgtcgtcgtc gaacaggtaa taccgaaatc gcggcatttc agcggctgaa tttcagagca
    33361 caggatccac accactggcg cggcgctcat cgcataaccg gcaatacaca tcatcgtcat
    33421 gccaacagag agccaggaca agccactgga agccgtaccg ttatcaaact gcatcaggca
    33481 atagcccagc accagagtgc ctaacgccat cacgctgaaa ccaattttca gagccggttt
    33541 acgacctgct ttatctaccg taaagaccgc aatgaaggtc gcgaacataa aggtcagtct
    33601 cacgaccaga gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca ttttgaagat
    33661 acgcggcgca tagtacatga tgatgttcat accggtaaac tgctgcatcg cctgcaacaa
    33721 cataccgaga aacacagcac gacggacgtt acggttgatc ttaaacagtg cccaaccgcc
    33781 ttgttttaac ttcaggcttt cacgaatttc gttgagttct tctcgcgctt tttccgacgt
    33841 atcgcgcagc atacgcaaca cttcttccgc ctcaatatga cgcccctttt ccgccagcca
    33901 gcgcgggcta tttggcagga agaccaccag aataatcagc aggactgctg gtaaagcaag
    33961 aacccccaac attgcgcgcc agttaccgct ataactgaac gctgtatcgg ataaaaacgc
    34021 cagcacgatg ccgagtgtga ccatcaactg gtacatactg atcatcttac cgcgaacgtt
    34081 ttcacttgcc atttcagaaa gatacagagg agcggtgtaa gacgcgatcc cgacagcaat
    34141 gcccagcacc acacgagcgg cgattaacat ctctacgctg gtcgcaaaag cggaccctat
    34201 agaaccgagt acaaacagga tggcccccgc catcaggctg tatttacgcc ccaggcggaa
    34261 cgacagccaa ccattaaaca gcgcaccgat tgctgcgccg agcatcatgc tactaaccac
    34321 ccattcctgc aaacgactgg tcagcacaaa gtgatcggta atgaacggca acgctccggc
    34381 gattacgccg atatcaagac caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa
    34441 catattcata cgccgcgtat cacgcaaaga acgtggcgtt aaagcagatt ccgtattgat
    34501 agtaaccatt ttttcctgcc agcagagagt aagacatagt gaaaaaatac gtgaacaatt
    34561 cacacaggtg tcaggtcgga aacagcataa atatggatta aattgctgcg acatgccgtt
    34621 atgtgatgga t
//
LOCUS       DAFNBJ010000034        34353 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000034 DAFNBJ010000000
VERSION     DAFNBJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.034"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            158..319
                     /locus_tag="L2X49_001589"
     CDS             158..319
                     /locus_tag="L2X49_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796692.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(403..1638)
                     /locus_tag="L2X49_001590"
     CDS             complement(403..1638)
                     /locus_tag="L2X49_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBN4796693.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDKFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(1785..1861)
                     /locus_tag="L2X49_001591"
     tRNA            complement(1785..1861)
                     /locus_tag="L2X49_001591"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1825..1827),aa:Pro,seq:tgg)
     gene            complement(1904..1990)
                     /locus_tag="L2X49_001592"
     tRNA            complement(1904..1990)
                     /locus_tag="L2X49_001592"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1954..1956),aa:Leu,seq:cag)
     gene            complement(2011..2086)
                     /locus_tag="L2X49_001593"
     tRNA            complement(2011..2086)
                     /locus_tag="L2X49_001593"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2051..2053),aa:His,seq:gtg)
     gene            complement(2145..2221)
                     /locus_tag="L2X49_001594"
     tRNA            complement(2145..2221)
                     /locus_tag="L2X49_001594"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2185..2187),aa:Arg,seq:ccg)
     gene            complement(2324..3709)
                     /locus_tag="L2X49_001595"
     CDS             complement(2324..3709)
                     /locus_tag="L2X49_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBN4796694.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3900..4640)
                     /gene="rffM"
                     /locus_tag="L2X49_001596"
     CDS             complement(3900..4640)
                     /gene="rffM"
                     /locus_tag="L2X49_001596"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBN4796695.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4643..5995)
                     /gene="wzyE"
                     /locus_tag="L2X49_001597"
     CDS             complement(4643..5995)
                     /gene="wzyE"
                     /locus_tag="L2X49_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBN4796696.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5992..7071)
                     /gene="rffT"
                     /locus_tag="L2X49_001598"
                     /gene_synonym="wecF"
     CDS             complement(5992..7071)
                     /gene="rffT"
                     /locus_tag="L2X49_001598"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBN4796697.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7068..8318)
                     /gene="wzxE"
                     /locus_tag="L2X49_001599"
     CDS             complement(7068..8318)
                     /gene="wzxE"
                     /locus_tag="L2X49_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBN4796698.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8320..9450)
                     /gene="rffA"
                     /locus_tag="L2X49_001600"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8320..9450)
                     /gene="rffA"
                     /locus_tag="L2X49_001600"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBN4796699.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVIST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9455..10129)
                     /gene="rffC"
                     /locus_tag="L2X49_001601"
                     /gene_synonym="wecD"
     CDS             complement(9455..10129)
                     /gene="rffC"
                     /locus_tag="L2X49_001601"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBN4796700.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10107..10988)
                     /gene="rfbA"
                     /locus_tag="L2X49_001602"
     CDS             complement(10107..10988)
                     /gene="rfbA"
                     /locus_tag="L2X49_001602"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBN4796701.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11007..12074)
                     /gene="rffG"
                     /locus_tag="L2X49_001603"
     CDS             complement(11007..12074)
                     /gene="rffG"
                     /locus_tag="L2X49_001603"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBN4796702.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12074..13336)
                     /gene="wecC"
                     /locus_tag="L2X49_001604"
     CDS             complement(12074..13336)
                     /gene="wecC"
                     /locus_tag="L2X49_001604"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBN4796703.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13333..14463)
                     /gene="wecB"
                     /locus_tag="L2X49_001605"
     CDS             complement(13333..14463)
                     /gene="wecB"
                     /locus_tag="L2X49_001605"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBN4796704.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14519..15565)
                     /gene="wzzE"
                     /locus_tag="L2X49_001606"
     CDS             complement(14519..15565)
                     /gene="wzzE"
                     /locus_tag="L2X49_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBN4796705.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15577..16680)
                     /gene="wecA"
                     /locus_tag="L2X49_001607"
     CDS             complement(15577..16680)
                     /gene="wecA"
                     /locus_tag="L2X49_001607"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBN4796706.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16920..18179)
                     /gene="rho"
                     /locus_tag="L2X49_001608"
     CDS             complement(16920..18179)
                     /gene="rho"
                     /locus_tag="L2X49_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBN4796707.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18264..18365)
                     /gene="rhoL"
                     /locus_tag="L2X49_001609"
     CDS             complement(18264..18365)
                     /gene="rhoL"
                     /locus_tag="L2X49_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBN4796708.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18506..18835)
                     /gene="trxA"
                     /locus_tag="L2X49_001610"
     CDS             complement(18506..18835)
                     /gene="trxA"
                     /locus_tag="L2X49_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBN4796709.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18966..20231
                     /gene="rhlB"
                     /locus_tag="L2X49_001611"
     CDS             18966..20231
                     /gene="rhlB"
                     /locus_tag="L2X49_001611"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBN4796710.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VEVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20365..21849
                     /gene="gppA"
                     /locus_tag="L2X49_001612"
     CDS             20365..21849
                     /gene="gppA"
                     /locus_tag="L2X49_001612"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBN4796711.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGAFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21896..23917)
                     /gene="rep"
                     /locus_tag="L2X49_001613"
     CDS             complement(21896..23917)
                     /gene="rep"
                     /locus_tag="L2X49_001613"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBN4796712.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24134..24583
                     /locus_tag="L2X49_001614"
     CDS             24134..24583
                     /locus_tag="L2X49_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBN4796713.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24782..25063
                     /gene="ppiC"
                     /locus_tag="L2X49_001615"
     CDS             24782..25063
                     /gene="ppiC"
                     /locus_tag="L2X49_001615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBN4796714.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25150..26625)
                     /gene="ilvC"
                     /locus_tag="L2X49_001616"
     CDS             complement(25150..26625)
                     /gene="ilvC"
                     /locus_tag="L2X49_001616"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBN4796715.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     SAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26775..27665
                     /gene="ilvY"
                     /locus_tag="L2X49_001617"
     CDS             26775..27665
                     /gene="ilvY"
                     /locus_tag="L2X49_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBN4796716.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(27662..29206)
                     /gene="ilvA"
                     /locus_tag="L2X49_001618"
     CDS             complement(27662..29206)
                     /gene="ilvA"
                     /locus_tag="L2X49_001618"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBN4796717.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29209..31059)
                     /gene="ilvD"
                     /locus_tag="L2X49_001619"
     CDS             complement(29209..31059)
                     /gene="ilvD"
                     /locus_tag="L2X49_001619"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBN4796718.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31124..32053)
                     /gene="ilvE"
                     /locus_tag="L2X49_001620"
     CDS             complement(31124..32053)
                     /gene="ilvE"
                     /locus_tag="L2X49_001620"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBN4796719.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32073..32336)
                     /gene="ilvM"
                     /locus_tag="L2X49_001621"
     CDS             complement(32073..32336)
                     /gene="ilvM"
                     /locus_tag="L2X49_001621"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBN4796720.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32333..33979)
                     /locus_tag="L2X49_001622"
     CDS             complement(32333..33979)
                     /locus_tag="L2X49_001622"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBN4796721.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(33982..34032)
                     /locus_tag="L2X49_001623"
     CDS             complement(33982..34032)
                     /locus_tag="L2X49_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796722.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34119..34217)
                     /gene="ilvL"
                     /locus_tag="L2X49_001624"
     CDS             complement(34119..34217)
                     /gene="ilvL"
                     /locus_tag="L2X49_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBN4796723.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8333 a         9453 c         8652 g         7915 t
ORIGIN      
        1 aaaacgctct cctgattttc caattcattt tctggataaa taaataattt atttttgtca
       61 ctattattta tgtaatcatc ctgtcaggga gagggatctc aattatcaat gcttaattac
      121 gtcatcattt tgatgacatg aaaccggagc attttgcatg gaattttcgt tttcacccaa
      181 acttcttgtt gttgctgtcg ccgccgctct tcctctcatg gccagcgcag cagatacccc
      241 gtcaactgcc accgcacgca aaggctttgc cggatacgat cacccaaacc agtatccaca
      301 gaacgctgca atttattgaa tttgcacgat catgtaggcc ggataaggcg tttacgccgc
      361 atccggcaat caaccgcagg cggccacaaa atggccgctt ttttacttac tcaccaccag
      421 caaatgtgcc tgcataatgt cactggccgg gcgaccgtgc gccagcaaat cagccattgc
      481 tttaagatat ggcggtagat ggcggaaata acgctgatac ccggcacaca aataattcag
      541 ccccggtttg ccgctggcat cgagcatgaa gcggtgtttc gggcagcctc cccagcacgc
      601 ttttaacacg ttacaactgc gacactgcgc cggtaattgc ttaaatttat cttcaccaaa
      661 cacctgctgt tgcggggaat cgatcatttc tgcaattgtt tgctgatgca tattccccag
      721 ccgatattgc ggataaacat agtgatcgca ggcgtaaacg tcgccgttgt gctcaacaat
      781 caccgagcgc ccacaggttg gctgatgatg gcaaaccgca cccggcgcac cgacaaaatt
      841 ggcaaacgcc cattcgatat tcatcacgaa aatcttgccg acgtcgcgtt tgatccagtg
      901 gtcgaatatc gccaccagaa attcaccaaa tttatcgggg cgcaccgacc attccgtcag
      961 ttccccctga atatcaccag gcgcatgcaa tttcagtcca tcgcgggcag ctgtttcatc
     1021 agccagacgc tcgaccaccg gaataaactg gatgaattcg actcccgcat cgcacaaaaa
     1081 atcataaacc tgcaacggtt gctgcgcgct ggtgcggttg acgcagacca gcacgttata
     1141 gtcgacatga tgtttttgca gcagcgtcag ggcacgcatc accagcttat gcgttggtct
     1201 gccacctttg gtcacgcgat attgattgtg gatctcagcc ggaccgtcca gcgataaccc
     1261 aacaagaaaa tgattttctg ccagaaatgc acaccattta tcatcgagca gcacgccgtt
     1321 agtctggaag ctgttactta tcttcctgcc agcaccgtat ttcgcctgta actttacggc
     1381 acggcggtaa aaatccagcc cgagtaacgt tggttcgccg ccctgccagg taaaagccac
     1441 ttcgttttgc ggttcgctgg cagcgatata gtgacggaca tacgcttcca gcgtgtcatc
     1501 gtccatatgc gtgactggct tttcgcggta aagggattgt ttttcgagat aaaaacagta
     1561 gtcacagttc agattacaat cggaaccact cggtttcgcc atcacatgaa aagcacgcgt
     1621 tggaacctgt tgcagcatgg tcactccttg cctgaagtca tccttcaata cgttaagggc
     1681 gaaatcatac cgtggggcaa tgagttatta cgggcgttta ttgcgaaggg agggtgacga
     1741 aatgcacaga aaacaaaaaa ccccgccgaa gcggggttca aaattggtcg gcgagagagg
     1801 attcgaacct ccgacccact ggtcccaaac cagttgcgct accaagctgc gctactcgcc
     1861 gaaatactgc tttttgaatt tttagttcaa ttctttaaag tcgtggtgcg agggggggga
     1921 cttgaacccc cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg
     1981 ccaccttcgc attgtcacaa cttctaataa tggggtggct aatgggattc gaacccacga
     2041 caactggaat cacaatccag ggctctacca actgagctat agccaccact acaaatcttg
     2101 ttacgcggta ttactaccac cgcagctcaa gcgccgggac taaatggcgc gcccgacagg
     2161 attcgaacct gagacctctg cctccggagg gcagcgctct atccagctga gctacgggcg
     2221 cttagcgccg ttgcgggggt ggataatacg gacttcccac ccctctgtct agtccctttt
     2281 taaataaatt gcgcgtttgg ttacggtttg tgcgttttgc tgcttattcc tccagtttat
     2341 gcgctttccc gtggcgatta aggccaaaaa ctttataaat cgccgtcacc gccagcataa
     2401 agatgatgcc aacaaacagc gacatacgcg tatcttcatt aaagtacatg ccgatcaaaa
     2461 cgcaaatcag gaatgccatt gttacgtagt tggcccaagg gaacaggatt gagcggaacg
     2521 gatggctggc aatcgccgct ttatgtgcac gccgaaaacg cagctggctt atcaatatca
     2581 caaaccatgg caccatcccc ggaagcacgc tggcactgta gacgtagaca aacacacgct
     2641 gcggattggg aatgatgtag ttcaggcatg agccaattag cagaatagca atagatactg
     2701 ccacacccgc aactggtacg ccgtgacggg aaactttcgc catcgccgcc ggtaactgac
     2761 ggtttttcgc cagtgcgtag agcatacgtc cgcaactgta catgccgctg ttacagccag
     2821 agagcgcagc cgtcagcacc acaaagttga taatgcccgc cgctgcggta ataccgattt
     2881 tggcaaaagt cagtacgaac gggctgccgt tgctgccaat ttcattccac gggaagatgg
     2941 tgacgataac gaaaatcgcg cctacgtaga aaatcaggat ccgccacagc actttgccta
     3001 cggcactgcg cagcgtcacc tgcggattct tcgcttcacc ggcagtaatg ccaatcagct
     3061 ccacgccctg gtaggacgcc accacaatac acagcgcggt caggaaccct ttccagccac
     3121 ccgcaaagaa accgccatgc tctgtgagat tgctaaaacc aatcgactgc ccgccattgc
     3181 caaagccaaa gaaaatcacg cccaggccaa tgacaatcat cacgataatc gtggtgactt
     3241 tgatcatcgc gaaccagaac tcgatttcgc cgtacaaccg caccgccgcc agattcgcca
     3301 acgccaccag cgccactgcg atcaatgcgg gtatccactg cgccatctcc gggaaccaga
     3361 actggacata aacgccaatg gcggtgattt ctgagatccc caccgccatc cacataaacc
     3421 aataagacca ggcggtgaga taaccaaaga acgggctcat ataacgatgc gcataaacgg
     3481 cgaacgaacc ggtaaccggt tcgaggaaca acatttcgcc cattgaacgc atgatgaaaa
     3541 agacgaatag cccggcgatg atataggcca acaatacgga tggcccggcc catttcaagg
     3601 tactggcggc ccccataaac aggccgacgc caatggtgcc ccccagggcg atgagttcga
     3661 tatgtcgagc ttccagccca cgctgtagct ctggtttgtt atctgccata aatcctcgtg
     3721 ttgtgtttgc atgctttccg gtgttaccgg ttatcgttat gggtacatcg agtgttgcga
     3781 atgttttcgt aattcaggag aaatggcaaa taaagcatta aaaatttgaa tgctttgtgt
     3841 aataaaaaag cagacaggcg acgaagtgac cactccgtcg ctttacaaag agaggaaaat
     3901 cagaggttgc cggtgtaatg ccagcgtaaa taacgcagca aacgaagctg acgcttaatg
     3961 cggctcggct gcgaaagcag gcggtagagc cactccagtc ccagcgtttg ccagattttc
     4021 ggtgcgcgtt ttacgtgacc ggtgaaaaca tcgtaagtcc cgccaacgcc catatacagc
     4081 gcatctggat gcaccagacg gcagtcgcgc atgaagatct cctgctttgg cgatcccatc
     4141 gcaacggtga cgatttgcgc tccgctggca tgaatgcgtt caaacagcgc ctgacgctgc
     4201 tcgggtttaa aataaccatc ctgactgcca acgatattca cattccattg gttgcgcagt
     4261 ttagcttcag tttgcgccag cacttcaggt ttaccgccca caagaaatac cggcgtccct
     4321 tctttgcctg cgcgcgccat cagctcttcc cagagatcgg caccggcaac gcgggaaacc
     4381 tgcgcctgcg ggtacttttt acgtactgaa cgtacaacgc tgatgccatc cgcatattta
     4441 aattcggcag cgttaattaa ctccctgacc tcggcgttat cttcaatggt cagcattttt
     4501 tcggcattaa tggcaaccag cgttccctgc ttaagctgcc cgtcagcaaa cagataatcg
     4561 agggcgtgct gcatatcacg ccaaccaatc aactgtaagc cacgcagcgt ataggtcggt
     4621 gccgtggtgt tgttattcat tgttatcctt caacctgcgt ccggagcgat gattttgtac
     4681 gtttatgaat gagtccggcg ctttcaaaaa gccagtacaa cagttttgcg atcatcagac
     4741 atgcgccgaa gaccacgata aaaaagacta cgcgtgagac aaacgaatcc agcccttcac
     4801 gtgccagcac gatcatattg aagatcgccc caaagcagaa actgtgcaat atcgcagcct
     4861 tatagcgatt agtctcgcgg ttgcccagct catacagcca gtcgaaccat ttgatgatca
     4921 gtccaaccac aatcgccccg agtgggatga acaacgcgcc gcccatcacc accagtgagc
     4981 ctataagcgt aggcgagatc gccagtccgg agtggttatt cagcacttcc caggtaaagt
     5041 agttggctga gttcagcacc atactcgggc gacccggcca cagccaggaa gggataaaga
     5101 catagaaatc gcggacaatt ggagccaggc cctggaagtc gatgttgtcg tagttctgca
     5161 acagcaacgc cagattctcc catggcgaga aggtgtcgcg agtgagatag agaaacgtat
     5221 agaacgcttc atcgccgctc acattcattc catagcgttt tagcgccagc cagaacatgc
     5281 ccacaatccc cagcacgccc gccgccgcca gcatccacaa cgaaatccag ccgcgaataa
     5341 tgccaataaa caggaagata gcgaatgcga tgatgatatt ggcgcgagtg ccgccgacaa
     5401 tcatataagt cagcaagcca aaggcgaccg tgctgacgag gaaaaacagc cacgctttgc
     5461 tatcctggcg cagaaagtag accaccagca tcgccgggat gaaaaagtaa aagaagcgtt
     5521 ttaacgccac gccggagact tcactggaga agatctgact gtaggagtta agccggaaca
     5581 gcaaaaagcc gttgtgcatg aagaagatgc cgacgcttac cagcgcgatg cccatcagga
     5641 tcacccacgt aagattggtc tccacgcggt tcatggtaaa cagcggacgg cgcggtacat
     5701 cagcaacgcg tttgcgtagg cgggttttgt aggtgacata gtaaaccgcg tagaagcagc
     5761 ccgcagaaag caacgcctgc aacaagattt ctggcggcgc gacaccaaca tcaaagcgaa
     5821 ataccagcac gctggtcagc gggaagccga agaaaaaggt gagcaaaaac agcaatgaaa
     5881 agaagacatt gaagttaaag cgcacacggc gaaactcaaa ccaggtcagc gtggcaataa
     5941 acagcgtgca gagcagccag acaacaaaca ggccactgaa ttgcagcaga ctcatgcgac
     6001 ctccccggcg gcaatcgcca acgcccgctg ccagccttgt agatagttag ggctaaagaa
     6061 ggcaatggtg tttttatcca ccgacgccaa ctggcgctgc gcttcacgca caatatcctc
     6121 gttgagatcg tcggtagtaa acagcaccgg caaatgctgt tccgtcatat cctgccagaa
     6181 cggattttcc cggttaagca cacaaggaat gcccgcctga atcagtaagc aaagcgtacc
     6241 aatgccctgc tggcgggcaa aaataaagta accaagatcg cactgacgaa gtagcgccag
     6301 ataggcgtca aattccagtt tttcgctcaa aatttgcaga ttttcttcgc tgaataactc
     6361 cagccccgcc tgacgaactt cctcaatgta cgcttcgtta ttaggcggat atcccatcgg
     6421 caccaccact tttaccgtat cgccaaattg ctgatgaacg gcgcacaagg cagcaatatg
     6481 gtcattgctg cggtcaccgg agttccccac cagaatggtc attttccctt cacgttgccg
     6541 atcgttcgcc atcgtattga gagaagggtc catccgcgtc gggaagtaca gcagttcgcc
     6601 ccgcaccttt ggatgcgttt tggcaaaaaa gctcaaatcg ccgcgggtgg caaatacaca
     6661 gccgactcgc ttttgcgcca ggcgacgtag tgggtaaaaa agcttatatc tcaagccact
     6721 ggaaagctca tacaggtctg ccccccagat atgccagaaa aactggctgg gcttaatgcc
     6781 accactcagc agagccagcc acagtgtggg attgaactga ccgtggaaga aaaaacgctg
     6841 ctgacggtta gcttttgctt tcgcgatgac cgcttccgcc agcgattttt tcccagggaa
     6901 aaattgcaca gaaagcgccg gacagctatc acttaagccg tcgtccttgc caacaaccat
     6961 aaactcgcgc gcgtgctcgc tcgtcgcggc cagcgcgtca ttgaaaaacc gcaaaacggt
     7021 tcggttatgg tgagggatat ccgatcccag tacgtgaatc agtacagtca tgcccgccta
     7081 cgccagagta aaaacacgcc acaacaaaga gaaaaataga cgatataagt tgccatatat
     7141 gcctgcgccg cgcccagtgc accatgcgca gggattagcc agtgggcaaa taccatcaat
     7201 aaagtgaact ggctgacttc cgccagaata taaaaccgca gtgacgcttt ggcgatcacc
     7261 agataaccaa agacataagc gcccactttt aacacatcac ccactaactg ccaggcaaag
     7321 agatcgcgca tagcggtaaa tttattcgac aacagcaacc agatagcaaa atcacgcagc
     7381 aaccagacgg taaaactcgc cgccgccact gccggtaaga cgaatttcag cgatttaacc
     7441 acttcccggg tgatatcgcg cttttccgtt agccgcgaca acgtgggcag caaatatacg
     7501 ctgaacgatg ccgtaataaa ttgcaggtag gcatcggaaa tactgctcac cccttgccag
     7561 atccccacct catcccagct atactgcgcc gccagcagtt tacgcatcat gatgtaagca
     7621 acaggcaagg tcaccgacgt aatcaacgcc atgagcgtaa atttgctcaa ctgccctgcc
     7681 agaccgttat cccagctggg tttcagatag cttaacggga tgacaccacg tttgatcaac
     7741 atgatggcgg caggaatcac caccagcgcg ggaatcagcg ccagacccag caacgcccct
     7801 tcataaccgc ccaaacggta actgacgtag tacgcgagaa cgccaatcaa gctgccgaca
     7861 atcagcgata acgcattacc tgcggcatcg cgaaagcctt tcatcagcgc cagtaacagg
     7921 ttgccccagg cgatccccat ttgcaccagc gccactaaac gcaccagccc ctgatagtcg
     7981 gtattaccaa acaatccctg gctgattggc gcagctgcca gcacaaaaac cagcgccatc
     8041 agcgtagaga aaccaagtac catcgctgat gaagtgccga ccacgcggcg cagctgttgc
     8101 ggattatcat ggtactgggc aacgtatttg gttacaccgt taaagatgcc agctccggca
     8161 agcacgccga gcacggtaat caactggcgg aaatttgccg ccagcccaag ccccgccgga
     8221 ccaaatgaca cagccagcaa cttaccgacc agtaacccgg caccaatctt gaccagtgta
     8281 ctggccgccg tccacaagga cgcttttgcc aacgacatat caggagaagt agttcaacaa
     8341 agtcgcaatt accgtacgct gattgacggg cgacaggttg tagaacagcg gcaggcgcag
     8401 caggcgctcg ctctctttgg tggtgtagcg atcttcaccg tggaactcac caaagcgttc
     8461 ccccgcaggg caaccgtgca gcggaatgta atggaacacc gccatgattt ccgcttcttt
     8521 cagaaagtta atcaacgcgc tccggtcatc aatatcccgc agtttaatgt agaacatatg
     8581 cgcgttctgc acgcagccat cgggaatcga cggcagctcg atacgcccgg ctttcgccag
     8641 aggcgctaac gcatcgtagt agttttgcca cagcgccaga cgttgctggt tgatacgatc
     8701 cgctgcttcc agttgcgccc acaggtacgc agcttgcaga tcggacatca aatagctgga
     8761 gccaatatcg cgccaggtat atttatcgac ctgaccacgg aagaactggc tgcggttagt
     8821 gcccttttca cggatgatct cggctcgttc gattaacgct ttatcgttaa tcagcgtcgc
     8881 gccgccttca ccacccgccg tatagttttt ggtttcatgg aagctaaagc agccaatatg
     8941 accaatggtt cccagtgcac gccctttgta agtggatatc acgccctgag cggcatcttc
     9001 taccacaaac agattatgct ttttcgccaa cgccataatg gtgtccattt cgcaggccac
     9061 acccgcgtaa tggaccggca cgataacgcg cgttttgtcg gtgatcgccg cttcaatcag
     9121 cgtttcgtcg atgttcatgg tgtccgggcg aacatccaca aaaacgattt ttgcgccacg
     9181 cagcacaaag gcattggcgg tggagacaaa ggtgtagctc ggcatgatca cttcatcgcc
     9241 aggctggata tcgagcagca gcgccgccat ctccagcgaa gcggtgcagg acggcgtcag
     9301 taacactttg gcgctgccaa aacgttgctc cagccactgc tggcagcgac gggtaaaacc
     9361 gccatcgcca cacagtttgc cgctacccat tgccgactgc atatagtcga gttcggttcc
     9421 caccaccggc ggtgcgttaa atggaatcat gtgatcacct gtataaccag tacgcggtgc
     9481 tttctacatt cgcaccactt tgtatgtatc gtttaagcgc ggcggtgttg cccatttggg
     9541 tcgccacccg caaagttgtt ttaccgcgag catacgccca gtttagcgcc gtttgcatca
     9601 gctcagcacc tacaccgcgt ccagccagca ggccaattcg cgcatctgtc gcattgagtt
     9661 cccgtaaaga gacataaccg cgaataccgc cggacgccgc acgtaaaatc agacattgat
     9721 gatcaaaggt gccgcgcacg gcattttcaa tccactgtgc ataaaagcgt ctgctggcgt
     9781 caggcgcata ccacggcgca cgaaaacggc tttgcgcaaa tgcggcgctg gctaactgac
     9841 gtaatgcggg aatatcggtc tcttgtgcca ctacagcacc actatcactg acattgttca
     9901 cgggtagtgc caaatcaact tcaccttcta ccagggagaa tcccagctgt tgcagggcat
     9961 ccagttcacc cgtatttgat gccgcaattt tggcctgcac ccgtgaccac ggcgctaaca
    10021 cgtctggcgt caggagcggt gcttcagacg taatgcgcac gatggcgctg ttaacaccaa
    10081 agaaggcgtt ttcccaggtt agtggctcaa tactggcgcg gacgggcacg aagtaactcc
    10141 agcagatatt ggccgtagcc agttttcgct aatgaactgg cagcacgctt cacaccctcg
    10201 tcatcgagcc agccgttacg ccaggcgatc tcttccaggc aggcaatctt aaagccctgg
    10261 cgtttttcta ctgtctgcac aaaggtactg gcttcaatca ggctgtcgtg agtgccagtg
    10321 tccagccagg caaatccgcg cccgagcagt tcaacggtca ggttgcccgc ctcgaggtac
    10381 atctggttga tggaggtaat ctccagttca ccacgctccg acggcttcac ctgctttgcg
    10441 tactccacga ctttactgtc gtagaaataa agcccggtca ccgcccagtt tgacttcggc
    10501 tgttttggct tttcttccag cgagatagcg cggaaattgt cgtcaaactc caccacgcca
    10561 aagcgttccg ggtccatcac ctgatagcca aataccgtcg ccccttccgt gcgcgccgca
    10621 acatgacgca gcttcggact gaagccctga ccgaagaaga tgttatcgcc cagcaccaga
    10681 caagaaggtt caccgttgag gaaggtttca ccgatgataa aggcctgcgc cagaccgtcc
    10741 gggctgggct gttcggcgta ttccagctgg ataccgaact cactaccatc gcccagcagg
    10801 cgctggaaat aacctttatc ttccggcgta gtgatgatga gaatttcgcg gataccggcc
    10861 aacatcagca ccgacagcgg atagtaaatc attggcttat cgtaaatcgg caacagttgc
    10921 ttcgatacgc cgcgcgtaat cggatgcaat cgggtgccgg aaccgcccgc caggataata
    10981 cctttcatgt ttgcctccgc tgaaaattag cctttcagac ctaaacgctc gccctgatag
    11041 ctgccgtcct gcacctgctt ccaccagctt tcattagcca gataccactg caccgtttta
    11101 cgcattccac tttcaaaggt ttcctgtggc agccaaccaa gttcacgggc aattttcgaa
    11161 gcatcaatgg cataacgcag atcatgcccc ggacggtcag cgacaaaggt gatcaagtca
    11221 cgatagtgcg ccacgccgtg cggcttgttc ggagccagtt cttccagcag ctcgcaaatg
    11281 gtttccacaa catcgagatt cttacgctcg ttgtgaccac caatattata ggtttcaccg
    11341 actttcccgg tggtcgccac gcaatacagc gcgcgggcgt gatcttccac atacagccag
    11401 tcacggattt gctgcccgtt gccatatacc ggcagcggtt tacccgccag cgcgttgagg
    11461 atcatcagcg ggatcagttt ttccggaaag tggtaaggac cgtagttatt cgagcagttg
    11521 gtgatcagcg tcggcagacc gtaggtccgc agccaggcgc gcaccagatg gtcgctgctg
    11581 gcttttgacg cggaataagg gctgctcggc gcatacggcg tggtttcggt gaagaaatca
    11641 tccgtcgagt gcaggtcacc atatacttcg tcggtggaga tatgatgaaa acggaacgct
    11701 gattttttat cttccgtcag cgtattccag taagcccgcg ccgcttcaag caatgtataa
    11761 gtcccgacaa tgttggtttc aataaacgct gccgggccgt caatagaacg gtcaacatgg
    11821 ctttcggctg ccagatgcat gacacagtct ggctgatgct cagtgaatac gcgtgccagt
    11881 tctgcccgat cgcagatatc aactttctca aaggcaaagc gctcgctttg cgcgaccggt
    11941 gccagcgaca tcaggtttcc ggcgtaggtc agcttatcga ccactaccac cgcgtcgctc
    12001 gtttcgttga tgatataacg caccagcgcc gagccaataa acccggcacc acctgttatc
    12061 agaatttttc tcatcagcgc cagactcctt tggcatcgac gacatactgc tgatggacat
    12121 tgtcgccatt gataactttg aactgactat gatcgaccag catcaccagc acgtctgccg
    12181 ttgccagcgc ctcgtcaagc tgcgccagag tacaaagccc ggtcagtttt ttcggcaact
    12241 ggtggatgtt aggctcaaca accagagttt cgccgctatg ccactgggcg atcagttcag
    12301 cgatttccat cgccgggctt tcgcgcaggt catcaatatt cggtttaaac gccagaccaa
    12361 agcaggcgat tttcagttca ctggcgcgtt tatcggtagc agccaggcaa tcagccaccg
    12421 ccgctttcac ctgatcgata acccagaacg gtttgtgatc gttcacttcg cgcgcggtac
    12481 ggataagccg cgcctgctgg gggttctgtg ccacgataaa ccacggatca acagcaatgc
    12541 agtgaccgcc cacgccaggg ccaggctgaa gaatattaac gcgagggtga cgattcgcca
    12601 ggcgaatcag ttcccagacg ttaatcccct gatcggcaca aatcagcgac aattcattag
    12661 caaaagcgat attcacatcg cggaagctgt tttcggtgag cttacacatt tccgccgtcc
    12721 gcgagttagt gacgacacac tcaccttcga ggaaaatttt gtacagttcg ctggcgcggg
    12781 ccgaacaaac cggcgtcata ccaccaatca cgcgatcgtt tttaatcagc tcgaccataa
    12841 cctggcccgg taacacgcgt tccgggcagt aagcaatgtt gacgtccgcc tgctcgccca
    12901 cctgctgtgg gaaagtgaga tccggacgca tctctgctaa ccattctgcc atcttctcgg
    12961 ttgaccccac cggcgaggtg gattcaagga tcaccagcgc gccttttttc agcactggcg
    13021 caatggagcg agcagccgat tcaacgtagg tcatatctgg ctcatgatcg cctttaaacg
    13081 gcgtaggtac agcaatcagc caggcatccg cttcaactgg cgtcgtgctc gctcgtaaaa
    13141 aaccgccttc tacggcagtt tttactacgc tcgccaaatc aggttcgacg atatggattt
    13201 cgccacgatt gatggtatca accgcgtgtt ggttgatatc gacaccaatc acctgttttt
    13261 gccgtgaggc aaacgcagct gctgtaggca gcccgatgta acccagtccg ataacagaaa
    13321 tggtcgcaaa actcatagtg atatccgatt attttttaac gcttccagaa tgcgagagca
    13381 tgcctgacca tcaccatacg ggttatgggc gcggctcata gcttgatatt cgttttcgtc
    13441 ttttaaaaga cgcgtcactt cctcgacaat tcgctgctta tccgtgccta ccagacgcac
    13501 cgtacccgcc gtcaccgctt ccggacgctc agtggtatcg cgcatcacca gcaccggttt
    13561 ccccagcgaa ggcgcttctt cctgaatgcc gcctgagtcg gtcaaaatca gccaggcgtg
    13621 gttcatcagc cagacaaacg gtaaatactc ctggggatcg atcagaatga catttttcac
    13681 atgccccaga atgcgattga ccggctctct gacgttcggg ttgagatgca ccggatagac
    13741 aatctggatg tcctggtgcg tggtggcgat gtctgccagc gcgtggcaga tttcttcaaa
    13801 gccacgaccg aaactttcac gcctgtgacc ggtcaccaga atcatctttt tatcggggtc
    13861 gataaacggg taatttgccg ccagttctga acgcagcgtg tcgctgctca tcacctgatc
    13921 acgcacccat aacagtgcat caatgactgt attaccagta atgaagattc ggctatccgc
    13981 aacgttttca cgcagcaagt tttgccggga agtttcggtt ggagagaagt gatacatcgc
    14041 cagatgcccg gtcaatgtac ggttagcctc ttccggccac ggcgaataga gatcgcccgt
    14101 gcgtaaacca gcctcaacgt gaccaacagg aatacgctga taaaacgccg ccaggctggt
    14161 tgccagcgtc gttgttgtgt cgccgtgaac cagcacgacg tctggtttga actcggcaag
    14221 aataggtttt agcccttcca gaatccgaca ggttatctct gtcaggccct gtcctggctg
    14281 cattatgttg agatcgtagt caggtacaat ggaaaagagt ttcagcacct gatcgagcat
    14341 ctcccgatgc tgcgcagtga cgcaaacttt agcctcaaaa aaaggatctt ttgccaacgc
    14401 atgcaccaac ggagccatct tgatggcttc cgggcgcgta ccaaatacag tcagtacttt
    14461 cacatcgatt ctcttcgaat aagcggcgag cgcctttgcg ctcgcagcag cagtgttgct
    14521 atttcgagca acggcgggtt aatgcgacac cagccccgat cagccccccg acaatgcccc
    14581 acataatcat caggaaggca cgacgtgggc tatcgcgttt taccggttct tccggcgtac
    14641 gcaaatagcg ataggtctga aaacgcggat ccagggttgg accaacattc agggtgttta
    14701 acatggcccg attctgatca tagtcgagat caaaggccgg accgacggcc tgtaaatttt
    14761 ccagtcgagc ctggagcatt ggacgcccaa gcaggaacat ttctgaatca ggtaattcct
    14821 cggcaggcac atctgtcgca ctgcgcgaaa tattatgctg ctcagcaatt ttcagcgcct
    14881 gctcaatgct gttcatccgg cggtcgtaga tggctttcgc cacctcttcc tgacgcttca
    14941 cctgagcttt catctggatg gtgcgtgccg cccatgcgcc tttcagctca tcattcagat
    15001 ggctggctgc acgctggctg gcaaaagcaa catactgacg taacaggtta ttagcgtcag
    15061 gcgcggtttc ggcaataagc ttcacgctgt cattgaccgc gcgggtaaag tctccgggga
    15121 taaactggat gttgttaatc atttcatcca gcaacgccgc atcggctttg ctgttgccca
    15181 ccatccgctg tttgtaatag tcggtttgca gccagaactc tctgcgggta tcccacgagg
    15241 ccagttgcat aacaaactct ttataggctt cgtccatgac cgatggttga tcggcagaag
    15301 ccatgtttga acggacatcc aggttacgca aaaattgctg ctgcgagtaa tatcccccca
    15361 gcatattcac cgttggacga tcggtaatcg ccgtcgagct ccactcctga cgagcaaaaa
    15421 aagtatacgc cagcgcgatt aacgcaaacg ccagccccat gccaataatc catagcttcc
    15481 cagcccacaa ggtacgaaac aacccacgaa tatccagttc attttcagcg tcttcggccg
    15541 gtttcccagg cattggttgt gtcatcacat cctcatttat ttggttaaat tggggctgcc
    15601 accacgattt ctacgcagtc tgcgttttac gcgcttaata aagcgagcaa ctttccaggc
    15661 acgcttaatg caatatccat agaggaagaa tgctagcaaa aagagcacca gcatgaccca
    15721 ctccgggaca aaatgagaat attctgccag cacgccaatg gaagcgagca gtgctgcggc
    15781 aagggtaatc agcacaaacg cctgacggga agtaaacccg gcacgcatga tcaaatggtg
    15841 aatatgctga cggtcaggag agaatgggct catgccttta cgcaggcgac ggtacataat
    15901 cgccaccata tccattagcg gaatggcgat tatccacaaa gcggtaaccg ggctgatggg
    15961 atgggttttg ccctgggtcg tttcgagcag gatccagata acggtaaaac caatcagcgt
    16021 actgcccgca tcacccataa agactttgta gcggcgaccc aggataccaa ggttaagcat
    16081 gatgtatggc aggatggcgg cgatcatcgc aaagcaccag attgcgaggc tggtttgccc
    16141 gtcgaaccac aaaatcatac cgattgctgc aaacgagacg caggacaacc cgcccagcaa
    16201 gccatcaatg ccatcaacca tgttgaacgc attaatggcc gcccagacgg caaatagcgt
    16261 caggaagtaa ccaaacggtc cgagcaccat ctcccaggag ccaaagatat aacccaggct
    16321 actgagataa agtttgccga acaccatcat aacaatgcca acagcggcct gtatggtggc
    16381 acggattttt acgctgatat caaaacggtc atccagcgcg ccaatgaaaa caagcacacc
    16441 ggcacaagcg agatagagag atgcatgcgg aatatagtaa tcgacaattc cgaacgtgaa
    16501 gcaaatccct gcgtaaaccg aaatcccccc aacgagaggt atcaatccct ggtgacgttt
    16561 gcggaagttt ggtttatcca ctaaaccgac tttttttgcc accttacggg caaaaaacag
    16621 aaacagtgtc gtgaataaaa aaatactgat gagatcagta ctcactgtca gtaaattcac
    16681 aatgcatgct ctcagagaaa attattagca gaagtataac cacgaagacc tttattcaga
    16741 agggaaagtc cgttccgaac ccaccagtcc tgtgcaaaaa ttaaccattt gatatgcaaa
    16801 ttgcataaca tgccagcaaa ttccaggaat tttcctaaca tcgccagcgc ggcattaaga
    16861 ttacagatat aaaagcaaaa cgccacgtaa acacgtggcg tttttggcat aagacaaatt
    16921 tatgagcgtt tcatcatttc gaagaaatcg tcattggtct tggtcatggc cagtttatta
    16981 atgaggaatt ccattgcatc gatttcgccc atcgggtgaa tgattttgcg caggatccac
    17041 attttctgca gttcttcctg agtcgtgagc agctcttctt tacgggtacc ggaacggttg
    17101 tagtcgatag ccgggaagac gcgtttttca gcgatcttac gagagaggtg cagttccatg
    17161 ttgcctgtac ctttaaactc ttcgtaaata acttcgtcca ttttagaacc ggtatcgata
    17221 agcgccgtcg cgataatggt caggctgccg ccctcttcca cgttacgcgc cgcaccgaag
    17281 aagcgtttcg gacgatgaag ggcattggcg tccacaccac cggtcaacac tttacctgac
    17341 gccggaacaa cagtgttgta agcgcgcgcc agacgagtga tggagtcgag cagaatgata
    17401 acgtctttct tgtgctcaac caggcgtttg gccttctcga tcaccatttc cgcaacctga
    17461 acgtggcgag atgcgggttc gtcaaaggta gaagcaacaa cttcaccttt taccagacgc
    17521 tgcatctcgg ttacttcttc cggacgttcg tcgatcagca gaaccatcag cacacaatcc
    17581 gggtggttgt aagcaatgct ctgagcaatg ttctgcagca gcatagtttt accggctttc
    17641 ggcggtgcca caatcagacc acgctgacca cgaccgatag gtgatgccag atccagtacg
    17701 cgagcggtta aatcttccgt agaaccgtta ccacgttcca tacgcagacg agagtttgcg
    17761 tgcagcgggg ttaagttctc aaagaggatt ttgttgcggg cgttttcagg tttgtcgaag
    17821 ttaacttcgt taactttcag cagcgcaaaa tagcgttcac cttctttcgg cgggcgaatc
    17881 ttaccagaga tggtatcacc agtgcggagg ttgaaacggc ggatttggct aggggaaacg
    17941 tagatgtcat caggaccggc gaggtaggag ctgtctgcgg aacggaggaa accaaatcca
    18001 tcctgcaata tctccagtac gccatcacca aagatatctt cgccactctt tgcgtgctgc
    18061 ttcaggatgg caaaaataat gtcctgctta cgcatacgag ccaggttttc cagccccata
    18121 ttttcgccga gagtgatcag ctcagaaacc ggcgtattct ttaattcggt aagattcata
    18181 gtggtgtgag ttcttaaact tggggtaaat ctcgaactta atgttgtgaa tggtatggca
    18241 ggatcatcca tgcctgttta cggtcatcga ctcatgtctt ttcgctgcct ggtcacagga
    18301 gagtacgcag aactgaaacg acaagacgga ttgagtgacg agccagaaat ctgttcactt
    18361 cgcatttaag ataaaacggg aagcgttgga aattacaaga ttcaaactta ataaggtatg
    18421 tttaatacga agtcaacact aagttagcat gactcacgcc gggcgtccag tttttagcga
    18481 cggggcaccc gaacatgaaa ttcccttacg ccaggttagc gtcgaggaac tctttcaact
    18541 gacctttaga cagtgcaccc actttggttg ccgccacttc accgtttttg aacagcagca
    18601 gagtcgggat accacggatg ccatatttcg gcgcagtgcc agggttttga tcgatgttca
    18661 gttttgcaac ggtcagtttg ccctgatatt cgtcagcgat ttcatccaga atcggggcga
    18721 tcattttgca cggaccgcac cactctgccc agaaatcgac gaggatcgcc ccgtccgctt
    18781 tgagtacatc cgtgtcaaaa ctgtcgtcag tcaggtgaat aattttatcg ctcatatata
    18841 actccacagg aataagcctg gcgtgttggt ttcgttgttg gtgtaacatt aaccaactaa
    18901 aggttgactt tatttcaccg gatacgcttt cgtaaagcaa tagtaagctg atattctacc
    18961 acactatgag caaaacacat ttaacagaac agaagttttc cgacttcgcc ctgcatccga
    19021 aggttgtaga agcccttgaa aaaaaagggt ttcataactg tacgcccatt caggcactgg
    19081 cccttccgct gacgctggcg ggtcgtgacg tagccgggca ggcgcaaacc ggtaccggga
    19141 aaacgatggc gtttcttacg tcaacgtttc attatcttct ctctcatcct gcgattgccg
    19201 atcgcaaggt gaatcaacca cgtgccttaa ttatggcacc gacgcgtgaa cttgccgtgc
    19261 agatccatgc cgacgcagaa ccgctggcgg aagctaccgg cctgaagctg ggtctggctt
    19321 acggtggtga tggctacgac aaacagctga aagtgctgga aagcggcgtt gacattctga
    19381 ttggcactac ggggcgttta attgactacg ccaagcagaa ccacattaac ctcggtgcca
    19441 ttcaggtgga ggtactggac gaagccgatc gcatgtacga tctgggcttt attaaagata
    19501 tccgctggct gttccgccgt atgccgcctg caaaccagcg cctcaacatg ctgttctccg
    19561 ccacgctttc gtaccgggta cgtgaactgg cgttcgagca gatgaacaat gccgaatata
    19621 ttgaagtgga accggaacag aaaacgggcc accgtattaa agaagagctt ttctaccctt
    19681 ctaacgaaga aaaaatgcgt ttgctgcaaa cgctgatcga agaagagtgg ccagacagag
    19741 cgattatttt cgccaatacc aaacaccgtt gtgaagagat ctggggccac ctggcggcag
    19801 atggtcatcg tgtcggtttg ttgacaggcg atgtcgcaca gaaaaaacgt ctgcgtattc
    19861 ttgatgaatt tacccgtggc gatctggata ttctggttgc caccgacgtt gccgcgcgtg
    19921 gtttgcatat tccggcagtg acgcacgtct ttaactacga tttacccgat gactgtgaag
    19981 attacgttca ccgtattggt cgtacaggtc gcgcaggcgc aagcggtcac tctatcagcc
    20041 tggcgtgtga agagtatgca ttgaatttgc ctgctattga gacctatatt ggtcactcaa
    20101 ttccggtaag caaatacaat ccggacgcat tgatgaccga tctgccaaaa ccgctgcgcc
    20161 tcacgcgccc acgtacaggc aatggtccgc gtcgtactgg cgctccgcgt aatcgtcgtc
    20221 gttcaggtta aataaaatgt catactcaaa atcattcaaa ctgtatcaag atggcaagag
    20281 aatgaatccc gatgagcata ctgtagtaag tgattcaggt gaaagaatgc agccaacaca
    20341 gagacagatt gaaggatgaa gagtatgggt tccacctcgt cgctgtatgc agccattgat
    20401 ctcggttcga atagttttca tatgctggtt gtgcgcgagg tggctggaag catccagacg
    20461 ctgacgcgaa ttaaacgcaa agtgcgtctg gctgctggcc tgaatagcga aaatgccctc
    20521 tctaatgaag caatggagcg cggttggcaa tgtctgcgcc tgtttgctga acgtctgcaa
    20581 gatatccctc cctcgcaaat tcgcgttgtc gctacggcga cattacgtct tgccgtcaat
    20641 gcgggtgctt ttattgccaa agcgcaggaa atcctcggtt gtccggtaca ggtgatcagc
    20701 ggtgaagagg aagcacgtct gatttatcag ggcgttgctc acacaactgg cggtgccgat
    20761 cagcgcctgg tggtggatat aggcggtgcc agtactgaac tggtaaccgg cacgggtgca
    20821 caaaccacct cgttgttcag cctgtcgatg ggctgcgtca cctggctgga acgctatttt
    20881 gccgatcgta atctggggca ggaaaatttt gatgctgcag aaaaagcggc acgcgaagtg
    20941 ttacgtccgg ttgccgatga attacggtat cacggctgga aagtgtgcgt tggtgcttcc
    21001 ggcaccgtgc aggcgttaca ggaaatcatg atggcacagg ggatggatga acgcattacc
    21061 ctggaaaagt tgcagcaatt gaaacagcga gccattcatt gcggtcggct ggaggaactg
    21121 gagattgacg ggctgacgct ggaacgtgcg ttagtgttcc cgagtggtct ggcgatcctg
    21181 atcgccattt ttaccgaact gaatattcag tgtatgaccc tggcgggcgg tgcgctgcgt
    21241 gaaggcctgg tctacggcat gttgcatctg gccgtcgagc aggatattcg cagccgtacg
    21301 ctgcgtaata ttcagcgccg ctttatgatc gatattgatc aggcgcagcg cgtagccaaa
    21361 gttgcggcta acttcttcga tcaggtggaa aaggaatggc atcttgaagc aataagccgc
    21421 gatttgctca tcagcgcctg tcagcttcat gaaatcggcc tgagcgttga ctttaaacaa
    21481 gcgccgcaac acgctgctta tctggtgcgt aatctggatc ttcccggttt tacccccgca
    21541 cagaaaaaat tgctggcgac gctactgctc aaccagacta atccggtcga tctctcatcg
    21601 ctgcatcagc aaaatgccgt accgccgcgc gtcgcagaac aactctgccg tttactccgc
    21661 ctggccatca tttttgccag ccgtcgccgt gacgatctcg tgccagagat gacattacag
    21721 gctaaccatg aactgttgac cttgacgctt ccgcaaggtt ggctaaccca acatccgctg
    21781 ggtaaagaga ttattgctca ggaaagccag tggcagagct atgtccactg gccgctggaa
    21841 gtgcattaat gagtaagtgc cggatgcgat gctgacgcat cttttccggc cttgattatt
    21901 tccctcgttt tgccgccatc atcgctttca gattcgccag atggctttgc cctttctgca
    21961 tccgttcttc ggcgctgacc actttgcgct cctgttccca aatcagatca tcctgcggca
    22021 gctccagcaa aaagcggctc ggctccgggc gcaccagctc gccgtactga cggcgttctt
    22081 tacacagcgt aaaggtcaat tccttctggg cgcgggtaat gccgacatag gccagccgcc
    22141 gctcctcatc gatattatct tcatcgatgc tgctctggtg cggcaaaaac ccttcttcca
    22201 taccgaccat gtagacataa ggaaactcca gcccttttga cgcgtgcaga gtcatcagtt
    22261 gcacctgatc cagctcttct tcactctcac cacgctccat catgtcgcgc aaagtaaagc
    22321 gcgtcaccac ctgggtgagc gtcatcggct catccagttc actgccttcc agcatctccg
    22381 tcatccagct aaacagttgg ttgacgttct tcatgcgcat ttcggcggct ttcgggctgg
    22441 gcgatgtttc gtacagccag gattcataat ccatgccatg gatcagatca cgcaccgcag
    22501 caatcggctc ccgctccgcc aggcgctgga tttctgccaa ccagtgagtg aagcgggtca
    22561 atgcttcata accacgtccg ctaagcgtct gactcaggcc catatcaaag ctggcggtaa
    22621 acatgctttt attgcgcgtc atcgcccact cacccagctt tttcagcgtc gccgggccaa
    22681 tctctcgctt cggcgtgtta acgatacgca gaaatgcgct gtcatcgtcc gggttagtca
    22741 gcacgcgcag ataagccagc aagtccttga tttcaggacg agagaaaaac gacgtaccac
    22801 cagaaatttt gtacgggatg cggttttgca tcaggaattt ttcaaacacc cgcgactgat
    22861 ggttaccgcg ataaagaatg gcgtaatctt tgtactgcgt tttattgacg aagtgatggg
    22921 cgatcagctc gccagtaacg cgctcggctt catgttcctc gttattcgcg cttaaaactt
    22981 ttagctctgt gccgtaaccc agttcggaga acagacgctt ttcaaagacg tgcggattat
    23041 tggcgatcag gatgttcgcc gctttcagaa tacgcccgga agagcgatag ttctgctcaa
    23101 gcttaatcac cttcagcgcc ggaaaatcct gactcagcag caccaggttt tgcggacgtg
    23161 caccgcgcca ggagtagatc gactggtcat cgtcacccac cacggtaaag cgcgcgcggc
    23221 tgcccaccag cagtttcacc agttcatact ggctggtgtt ggtgtcctga tactcatcca
    23281 ccagcaggta gcgaattttg ttctgccagc gttcgcgtac ttcttcattg cgttgcagca
    23341 gcaacgtcgg caataaaatc aggtcatcga agtcgagaac gttacaggct ttcaggtgtg
    23401 catcatacag cccataacaa tgggcaaaaa tacgatcccg ctcgcccttt gcctctgccg
    23461 ccgcctgcgc cggagttttg agatcgttct tccagttaga gatggtcgaa atcagttgtt
    23521 gcaggagaac tttgtcatct tcaatcagcc cctcggtcaa ctctttaagc aaagcaagct
    23581 gatcggtatc gtcaaacaac gagaagttcg ctttcatccc aagcgccgca tactcgcgtt
    23641 tgatgatatc cagccccaac gtatggaaag tggagatcat cagcccacgc gcctctttgc
    23701 gccccagcgt ctgccctaca cgctctttca tctcgcgcgc cgccttattg gtaaaggtta
    23761 ccgccgcgat atgtcgcgcc tgataaccgc aaccgcggat cagatgggcg attttattgg
    23821 tgataacacg agttttaccg gaacccgcgc ccgccagcac caggcagggg ccggtaacga
    23881 attcgacagc ttgttgttgg ccggggttta gacgcatagg tgtattgctc aatcttcgaa
    23941 cggggggagg attgtagcag aatcgcattt gaatgtgacc gcgtgtaagc cgctaaccgg
    24001 ttggctgcgg atgtttaaat cagagagaga agaccgaaat cggagttcat cgtgaatcca
    24061 ttagacttag aaaatatcgg gtcgtaacgt tgtgttatta ctttttcaga gtttactaag
    24121 gagaggttta ctcgtggcga ttaatttcag ccctaaggtt ggtgaaatac tggaatgcaa
    24181 ctttggtaac tatcctgtta gccaaaatgg accgttcagc acgacgtatt acgatggccg
    24241 cattcctcct gaaatgataa aaaacaggtt agttgtcgtg ctcaacggaa aaattaatgg
    24301 caacgcatgt atcgtcgttc cattatcaac cactcgcgat catgacaagt taaatcgtgg
    24361 aatgcatgtt gaaatagcca gtaatgttat caatggcctg caattctttg atcagcagat
    24421 tcgttgggcc aaagccgacc tggtacaaca agtcagtcga aaccgattaa atagagcaag
    24481 gacttacaga gggtatctta atcaatgttt gccacatgag ttggttgcag acatacagag
    24541 ggcggtcatc aagtcaatca atgccatatc gctcataaat tgacaataaa ataaccatcc
    24601 ccgataatga ctttgtagcc catagaggcc ctaagttttc tccccttgag ggacctgcga
    24661 agcccggatt ttccgggctt ttgccatata tgtaccagcc cctccctgac tgacaattac
    24721 gttagcatct gcggatcatg ctaaaatcgc cgcctgacaa ttattcactc aaggcacgat
    24781 catggcaaaa acagcagcag cactgcatat ccttgtaaaa gaagagaaac tggctctgga
    24841 tcttctcgag cagattaaga acggtgccga tttcggcaag ctggcgaaga aacactccat
    24901 ttgcccatca ggcaaacgcg gcggtgattt aggtgaattc cgccagggtc agatggttcc
    24961 ggcgttcgat aaagttgtgt tctcttgtcc ggtactggag ccgaccggcc cgctgcacac
    25021 ccagttcggt taccacatca ttaaggtgct gtaccgcaac taatagcaag gccttctcca
    25081 ggagaaggcc ttgagtgttt tctccctctc cctgtgggag agggtcgggg tgagggcatc
    25141 agcgcgcact taacccgcaa cagcaatacg tttcatatct gtcatatagc cgcgcagttt
    25201 cttacctacc tgctcaatcg catggctgcg aatcgcttcg ttcacatcac gcagttgcgc
    25261 gttatctacc gcgctttccg gaatagcttt acccaagtcg cccggttgca gctctgccat
    25321 aaacggtttc agcaacggca cacaagcgta agagaacaga tagttaccgt actcagcggt
    25381 atcagagata accacgttca tttcgtacag acgcttacgg gcgatggtgt tggcaatcag
    25441 cggcagctcg tgcagtgatt cataatatgc agactcttca atgatgccgg aatcgaccat
    25501 ggtttcgaac gccagttcaa cgcccgcttt caccatcgcg atcatcagta cgcctttatc
    25561 gaagtactcc tgctcgccga ttttgccttc atactgcggc gcggtttcaa acgcggtttt
    25621 gccggtctct tcacgccagg tcagcagttt cttatcatcg ttggcccagt ccgccatcat
    25681 accggaagag aattcgccgg agatgatgtc gtccatatgt ttctggaaca gcggtgccat
    25741 gatctctttc agctgttcag aaagcgcgta agcacgcagt ttcgccgggt tagagagacg
    25801 gtccatcatc agggtgatgc cgccctgctt cagcgcttcg gtgatggttt cccaaccgaa
    25861 ctgaattagt ttttctgcgt atgccggatc ggtgccctct tccaccagct tgtcgaagca
    25921 cagcagcgag ccagcctgca acataccgca caggatggtt tgctcaccca tcaggtcaga
    25981 tttcacttcc gcaacgaagg acgattccag cacgcccgca cggtgaccac cggttgctgc
    26041 cgcccatgct ttagcgatcg ccataccttc gcctttcgga tcgttttccg ggtgaacggc
    26101 aatcagcgtc ggtacgccga atccacgttt gtactcttca cgcacttcgg tgccagggca
    26161 tttcggcgca accatgacta cggtgatatc tttacggatc tgctcgccca cttcgacgat
    26221 gttgaaaccg tgcgagtaac ccagcgccgc gccgtctttc atcagtggct gtacggtgcg
    26281 cactacatca gagtgctgct tgtccggcgt caggttaacc accagatccg cctgcgggat
    26341 cagttcttcg taagtaccaa ctttaaaacc gttttcggtc gctttacgcc aggatgcgcg
    26401 cttctcggca atcgcttctt tacgcagagc gtaggagata tcgagaccag aatcacgcat
    26461 gttcaggccc tggttgagac cctgtgcgcc acagccgacg atgaccactt ttttaccctg
    26521 aaggtagctc gcgccatcgg cgaattcatc gcgccccata aagcgacatt tgcccagctg
    26581 tgccagctgc tggcgcaggt tcagtgtatt gaagtagtta gccatggtga ttcctcgtga
    26641 tgttgtgctt cttattgttc ggttcgcgtt tgcgagattg aattcactat atgacaggaa
    26701 atttattgcg gaaattgata tattcacaac gtcacattgc aatttctgca acgtcaacat
    26761 tgagggctgc gccattggat ttacgcgatc tgaaaacctt cctgcatctg gcggaaagcc
    26821 gccattttgg ccgcagcgcg cgggcgatgc acgttagccc gtccacgctc tcacggcaga
    26881 ttcagcgcct ggaagaagat ctcggtcagc cgctgtttgt gcgcgataac cgcacggtga
    26941 cgctgactga agcgggcgaa gagttgcgcg ttttcgccca gcaaacgctg ttgcagtatc
    27001 agcagttgcg ccacaccatc gatcagcaag ggccatcgct ctctggcgaa ttacatatct
    27061 tctgctcggt gaccgctgcc tacagccatc tgccgccgat tctggatcgc ttccgcgcgg
    27121 aacacccgtc ggtggagatt aaactcacta ctggtgatgc ggcagatgcg atggaaaagg
    27181 tagtcactgg cgaagcggat ctggcgattg cgggtaaacc ggaaaccttg cccggcgcag
    27241 tggcgttttc gatgctggag aatctggcag tagtgctgat tgcccccgcg ctgccctgcc
    27301 cggtgcgtaa tcaggtgtcg gtagagaagc cggactggtc aacggtgccg tttatcatgg
    27361 ccgatcaggg gccggtacgc cgccgcattg aactgtggtt tcggcgtaac aaaatcagta
    27421 acccgatgat ttacgccacg gttggcgggc atgaagcgat ggtatcgatg gtggcgctcg
    27481 gctgtggcgt ggcgctgttg ccggaagtgg tgctggaaaa cagcccggaa ccggtgcgta
    27541 accgcgtgat gatactagag cgaagcgatg agaaaacgcc gtttgagctg ggcgtgtgtg
    27601 cgcagaaaaa gcggctgcat gagccgctga ttgaagcgtt ctggaagatt ttaccgaacc
    27661 actaacccgc caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg caatcgtagc
    27721 ccagttcatt cagccgggtt tcgaaatccg gttcatggtc gccaagctcg aacgccgcca
    27781 gtacgcgccc gtagtcggta ccgtgactgc gataatggaa cagagaaata ttccagtgcg
    27841 tacccagcgt gttgaggaag cgcagcagcg cacccggcga ttccggaaat tcgaagctgt
    27901 agagacgttc ctgcaacgga tgcgatggac gcccgccaac catatagcgc acatgtagct
    27961 tcgccatttc atcgtcggag agatcaacca cgctgtagcc gccgtcgttg agcatctgca
    28021 aaatttcttt gcgctcttcg aggccgcggc ttaagcgcac gccgacaaag atgcaggcgt
    28081 ttttggcatc ggcaaagcgg tagttgaatt cggtgaccga acgtccgcca agcaactggc
    28141 agaatttgag gaagctacct ttctcttccg gaatggttac cgccagcaac gcttcacgct
    28201 gttcacccag ctcacaacgt tccgagacgt agcgcaggcc gtgaaagttc acgttagcac
    28261 cggaaagaat atgcgccagc cgttcgccgc gaatgttgtg ctgggcgata tattttttca
    28321 ttcccgccag cgccagcgcg ccagagggtt ccgccaccgc acgcacatct tcgaataaat
    28381 ccttcatcgc cgcacagatc gcatcgctat cgacggtgat gatgtcgtcg agatactcct
    28441 ggcataaacg gaaggtttcg tcaccgatgc gttttaccgc gacaccttcg gcgaacagtc
    28501 ccacgcgtgg cagatcaacc ggatgacccg catccagcgc tgctttcagg caggcggagt
    28561 cttccgcttc tacggcgatc actttgattt gcggcatcag ttgtttgatc agcaccgcta
    28621 cgcccgccgc cagaccgccg ccgccgactg gcacaaatac gcggtcgaga tgggcgtcct
    28681 gctggagcag ttccagtgcc agcgtacctt gcccggctat caccatcgga tggtcgaacg
    28741 gcggcaccca ggtgaagccc tgctgctgcg acagttcgat tgctttggct ttcgcttcgt
    28801 caaagttcgc gccatgaagc agcacttcgc cgccgaatcc acgtaccgca tcgactttga
    28861 tatcggcggt ggcggttggc atgacgatca gcgccttcac gcctaaccgt gcggaagaaa
    28921 acgccacacc ctgcgcgtgg ttacccgcag aagcggtgat cacgccgtgc gctttttgtt
    28981 cttccgtcag gcccgccatc atggcgtacg cgccgcgcag cttaaagcta tgaactggct
    29041 ggcgatcttc gcgcttcacc agaatgacgt tatcaagacg cgacgacagt ttttccattt
    29101 tctgtagcgg cgtgacctgc gccgcttcgt agaccggcgc gcgcagcacc gctcttaaat
    29161 attcggcacc ttcaggggca ccggacaggg gttgtgagtc tgccatcatt aaccccccag
    29221 tttcgattta tcgcgcaccg cgcctttgtc ggcgctggtt gccaggctgg cgtaggcacg
    29281 cagcgcaaag gagacctgac gttcacgatt tttcggcgtc caggctttgt caccccgagc
    29341 ttcctgcgct tcacgacgcg ccgccagttc ggcatcgctt acctgtaact gaatgccacg
    29401 gttcgggatg tcgatagcga tcaggtcacc atcttcaatc agaccaatgc tgccgccgct
    29461 tgccgcttcc ggtgagacgt ggccgatgga aagaccagag gtgccgccag agaaacgacc
    29521 gtcggtgatc agcgcacagg ctttaccgag acccattgat ttcaggaagc tggttgggta
    29581 gagcatttcc tgcatgccag ggccgccttt cgggccttca tagcggatca ccaccacatc
    29641 gcccgcgaca actttgccgc cgagaatcgc ttctaccgcg tcgtcctggc tttcgtacac
    29701 tttcgccggg ccggtgaatt tgaggatgct gtcatcgacg cccgcggttt taacgatgca
    29761 gccgttttct gcgaaattac cgtagagcac cgccaggccg ccgtctttgc tgtaggcgtg
    29821 ttccagcgag cggatacagc cattggcgcg atcgtcatcg agagaatccc aacggcaatc
    29881 ctgcgagaat gcctgtgtgg tacgaatgcc tgcaggacct gcgcggaaca tattttttac
    29941 cgcgtcatcc tgggtcagca taacgtcgta ttgttccagc gtttgcggca acgtcaggcc
    30001 aagtacgttt ttcacatcac ggttcagtaa ccccgcgcga tccagttcgc cgagaatacc
    30061 gataacacca ccagcacggt gaacatcttc catatggtat ttctgggtgc tcggcgcaac
    30121 tttacacagc tgtggaacct tgcgggaaag cttatcgata tcactcatgg tgaagtcgat
    30181 ttccgcttcc tgcgccgccg ccagcaggtg aagtactgtg ttagtcgatc cacccatcgc
    30241 gatatccagc gtcatggcgt tttcaaacgc cgccttactg gcgatattac gcggcagtgc
    30301 actttcgtcg ttttgctcgt aataacgttt ggtcaattca acaatgcgtt taccagcatt
    30361 aaggaacagc tgcttacggt cggcgtgggt tgccagcagc gagccgttgc ccggctgcga
    30421 caggcccagc gcttcggtca ggcagttcat tgagttagcg gtaaacatcc cggagcagga
    30481 accgcaggtc ggacacgcgg aacgttcaac ctgatcgctc tgggagtcag atactttcgg
    30541 gtctgcgccc tggatcatcg catcaaccag atcgagcttg atgatctgat cggaaagttt
    30601 ggttttcccg gcctccatcg ggccgccgga aacaaagatc accggaatat tcaggcgcag
    30661 ggaagccatc agcatccccg gggtgatttt gtcgcagtta gagatgcaga ccatggcgtc
    30721 ggcgcagtgg gcgttgacca tatactcaac ggaatcagcg atcagttcgc gagatggcag
    30781 tgaataaagc atccccccgt ggcccatggc aatcccatca tccaccgcaa tggtgttgaa
    30841 ctctttggca acgccgccag ccgcttcaat ttgttcggcg accagtttac cgagatcgcg
    30901 cagatggacg tgacccggta caaattgggt gaacgagttc acaaccgcga taatcggctt
    30961 gccaaaatcg gcgtcggtca ttccggtggc gcgccacagc gcacgagcac ccgccatatt
    31021 acgaccatga gtggtggtgg cggaacggta cttaggcata ctttatttac tcccagtgtc
    31081 tgtctcgtaa atgggacggt gcgtgccgtc ccattttttg tatttattga ttaacttgat
    31141 ctaaccagcc ccatttatct tcggtttcgc cagtgaagag gccgaagaag gcttgctgaa
    31201 tgcgtttggt aaccgggcca caacggcctt cgccaacctg aataccgtct acgctgcgca
    31261 ctggcgtgat ttctgccgca gtaccggaca taaacacttc atccgccagg tacagggatt
    31321 cgcgcgacag aacctgctca cgcacttcaa ttcccagctc tttcgccagt ttgatgatgg
    31381 catcacgggt aatacccggc agcgcggagg aggtgaacgg tggggtgaac agcacaccat
    31441 ctttcacttc aaacaggttt tcgcctgcgc cttcagagat ataaccgttc acatccagcg
    31501 cgataccttc ctgataaccg tggcggcgcg cttcgctacc caccagcagg gaagagagat
    31561 agttaccacc ggcttttgcc gcggttggga tggtgtttgg tgctgcgcgg ttccaggagg
    31621 aaaccatcgc atcgatcccc tgctccagcg cttctgcgcc cagatacgct ccccacggga
    31681 aagcggcgat aatcacatcg gttgagtatc ccgctggcgg gtttacaccc atgccaacat
    31741 caccaacgaa gatcagcgga cggatatagg cgctggtgag attgtttttg cggatcacgt
    31801 cacgacaagc ttccatcagc tcatcaatgc tctgcgaaac cgggaagcga tagattttgg
    31861 cggagtcatg cagacgctgc atatgctcac gatggcggaa tacaaccggt cctttgtgcg
    31921 agtcgtagca acggatgcct tcaaaaaccg aagtgccata gtgcagcgcg tgcgacatca
    31981 catgcacctt cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg taatcagctt
    32041 tcttcgtggt catttttata ttccttttgc gctcaggcgc ggatttgttg tgatgtggtt
    32101 gtgctctgac agatggcaac gtgtgcgacg tccaccagtt tatttaactg actaaacagt
    32161 aagtcgaccg accgtgggct ggcaacggtc aattcgatat ttatattttg tgcatcgctg
    32221 gcggcggcca tattcattga gcagacgtgg aaaccacgat gacgcaccac gcgtaaaaca
    32281 cgttctaagg tttctggatt gaagcgagcc gatacattga cctgatgttg catcatgata
    32341 atttctccaa catttctgaa ttactggcgc caggcggcac cagcggccag acgttctcaa
    32401 gttcgtcgat tgagacatga agcaggtatg gcccatcact gttcagcatg gtgtcgagtg
    32461 ccgcttcaac ctggtcttta cgggtgatgt gttggccagg gatgccgaag gcgctggcta
    32521 acatgaggaa atcggggtta tcagtaaggg tggtttcgct gtatcgttcc tgaaaaaaca
    32581 gttgctgcca ttgtcgaacc atccctaacc gttggttatc gagtaagacg attttcaacg
    32641 gtaactgctt gcgttttacg gtgcccagct cttgcacatt catcatgaaa gagccgtcac
    32701 cggagataca gacaacggta tcgttcggtc gcgcgacttg tgcgccaacc gccgccggta
    32761 aaccaaaacc catggtacct aagccgctgg aggtgatgaa attttccggg cgagtgtggg
    32821 cgatgtgctg cgcagcccac atctggtgct gccccacatc tgtggtcacg acgcaatccg
    32881 caggtttacg atccgacagt tgttttaaca acaatggcgc gtagatagcg tcaccgggat
    32941 ggtcgtaacg ccaggcatgt tcatcacgca gctgcgcgca gtgtagctgc cagtcattga
    33001 tatttaacgg ctgctgtaat gctggtaaca gagcatttaa atcaccttgt aatgccacat
    33061 gtgcctgacg cagcttgttc atttctgccg ggtcgatatc catatggata acactggcgt
    33121 gtggtgcgaa ggtgttcagt ttgccggtca cccggtcatc aaaacgtgca cccacggcga
    33181 tcagcaagtc gcactcctgc accgcgaagt tcgccgcttt ggtgccatgc attcccagca
    33241 tgcccagata gtacggataa tctgcttcaa ctgcgcccag ccctttcagc gtgcaggtgg
    33301 caggcatttt tgtggtagcg agaaattctc gtaaagcagg aactgcctgc gccataccca
    33361 cgccaccacc aacgtacagc atcggttttt gcgcttttgc cagcatctgg cgcgcttgct
    33421 cgacttcggc atgtgggaaa gtcacttcgt tttcaacggt ggtgaaccac ggttccaggt
    33481 caccgctggc tagctggata tcttttggga tatcgaccag aaccggacca ggacgacctg
    33541 agctggcaac gtcgaatgct tcagccatga tgcgcggcaa ttcttccagc gactgcacca
    33601 gaaagctgtg cttggtacag gctaacgaca atcccaggac atccacttcc tgaaatgcgt
    33661 cagtgccgat aaacggtgcg gacacttgac cggtgatggc aacaacaggg atagaatcta
    33721 acagtgcgtc cgcaagcccg gttatcaggt tggttgcgcc cggaccagac gtggcgatac
    33781 atacgccagt tttgccggta gcacgggcat aaccgatagc cgccattgcc gcaccctgct
    33841 catgtcggca cagcaagtgc tccacgccgc cgtcatacaa tgcatcgtaa accggcataa
    33901 ttgcgccacc cggataaccg aaaacggtgt tcacaccctg tgcccgcaac gcatgtacca
    33961 cccactgtgc gccattcata gttagttccc cgtcctgaat cttgagaaac agaattttgt
    34021 gctgttattc attgtctgct cctcggttat gtttttaagg tcaaaaaaaa cccccggacc
    34081 tttcggtgcg ggggtcttag ttcgttaagg cttgatctct aagcctttcc tcgtccaagt
    34141 gcagccccgc acggtgggat aataatcacc accacgctaa tcacgaccag gctaatcact
    34201 cgtagaaggg ctgtcatttt gtcttttctt gcatcttgtt cgaaggaatg cctaaagagt
    34261 taccataggt tttgtaaata gtacaagata ttttttggcc gatttttatg atgcgtttaa
    34321 taatttcaat ataaattgtt gttttttaaa aat
//
LOCUS       DAFNBJ010000035        34096 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000035 DAFNBJ010000000
VERSION     DAFNBJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.035"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            18..734
                     /locus_tag="L2X49_001625"
     CDS             18..734
                     /locus_tag="L2X49_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBN4796724.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            764..1264
                     /locus_tag="L2X49_001626"
     CDS             764..1264
                     /locus_tag="L2X49_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4796725.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1356..4040
                     /locus_tag="L2X49_001627"
     CDS             1356..4040
                     /locus_tag="L2X49_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBN4796726.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4037..5125
                     /locus_tag="L2X49_001628"
     CDS             4037..5125
                     /locus_tag="L2X49_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBN4796727.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5213..6511)
                     /gene="exuT"
                     /locus_tag="L2X49_001629"
     CDS             complement(5213..6511)
                     /gene="exuT"
                     /locus_tag="L2X49_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBN4796728.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6994..8406
                     /gene="uxaC"
                     /locus_tag="L2X49_001630"
     CDS             6994..8406
                     /gene="uxaC"
                     /locus_tag="L2X49_001630"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBN4796729.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8421..9908
                     /gene="uxaA"
                     /locus_tag="L2X49_001631"
     CDS             8421..9908
                     /gene="uxaA"
                     /locus_tag="L2X49_001631"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBN4796730.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9990..10541
                     /locus_tag="L2X49_001632"
     CDS             9990..10541
                     /locus_tag="L2X49_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBN4796731.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAVRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10546..11790)
                     /gene="sstT"
                     /locus_tag="L2X49_001633"
     CDS             complement(10546..11790)
                     /gene="sstT"
                     /locus_tag="L2X49_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBN4796732.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12189..13154)
                     /gene="alx"
                     /locus_tag="L2X49_001634"
     CDS             complement(12189..13154)
                     /gene="alx"
                     /locus_tag="L2X49_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBN4796733.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13437..14423)
                     /locus_tag="L2X49_001635"
     CDS             complement(13437..14423)
                     /locus_tag="L2X49_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBN4796734.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAQGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14502..15185)
                     /locus_tag="L2X49_001636"
     CDS             complement(14502..15185)
                     /locus_tag="L2X49_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBN4796735.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAQK"
     gene            complement(15262..15765)
                     /locus_tag="L2X49_001637"
     CDS             complement(15262..15765)
                     /locus_tag="L2X49_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBN4796736.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15850..16986
                     /gene="rlmG"
                     /locus_tag="L2X49_001638"
     CDS             15850..16986
                     /gene="rlmG"
                     /locus_tag="L2X49_001638"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBN4796737.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEDTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            complement(17104..19122)
                     /gene="fadH"
                     /locus_tag="L2X49_001639"
     CDS             complement(17104..19122)
                     /gene="fadH"
                     /locus_tag="L2X49_001639"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBN4796738.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSAFQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVAEDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHLKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAKGTRLALEI"
     gene            complement(19548..21899)
                     /gene="ygjK"
                     /locus_tag="L2X49_001640"
     CDS             complement(19548..21899)
                     /gene="ygjK"
                     /locus_tag="L2X49_001640"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBN4796739.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPVYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLFMLYNDFFRKQ"
     gene            complement(21916..22986)
                     /gene="ygjJ"
                     /locus_tag="L2X49_001641"
     CDS             complement(21916..22986)
                     /gene="ygjJ"
                     /locus_tag="L2X49_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HBN4796740.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEVRPAEHDDTKTPAITSMS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23120..24553)
                     /locus_tag="L2X49_001642"
     CDS             complement(23120..24553)
                     /locus_tag="L2X49_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBN4796741.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYINGLTAEERHIEATPASNVD"
     gene            complement(24616..25065)
                     /locus_tag="L2X49_001643"
     CDS             complement(24616..25065)
                     /locus_tag="L2X49_001643"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBN4796742.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25062..28154)
                     /gene="ebgA"
                     /locus_tag="L2X49_001644"
     CDS             complement(25062..28154)
                     /gene="ebgA"
                     /locus_tag="L2X49_001644"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBN4796743.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYPQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28338..29321)
                     /gene="ebgR"
                     /locus_tag="L2X49_001645"
     CDS             complement(28338..29321)
                     /gene="ebgR"
                     /locus_tag="L2X49_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBN4796744.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFVEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLSREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29540..29872
                     /locus_tag="L2X49_001646"
     CDS             29540..29872
                     /locus_tag="L2X49_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBN4796745.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            complement(29914..31293)
                     /gene="ygjG"
                     /locus_tag="L2X49_001647"
     CDS             complement(29914..31293)
                     /gene="ygjG"
                     /locus_tag="L2X49_001647"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBN4796746.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            31711..33231
                     /gene="aer"
                     /locus_tag="L2X49_001648"
     CDS             31711..33231
                     /gene="aer"
                     /locus_tag="L2X49_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HBN4796747.1"
                     /translation="MSSHPYVTQQNTPLANDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLTAMLWFVAAPVVTYFLCTLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33385..34008)
                     /gene="nfeR"
                     /locus_tag="L2X49_001649"
                     /gene_synonym="yqjI"
     CDS             complement(33385..34008)
                     /gene="nfeR"
                     /locus_tag="L2X49_001649"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBN4796748.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
BASE COUNT         8456 a         8667 c         8904 g         8069 t
ORIGIN      
        1 tgtaagatca aatttatatg gtggcgaaaa ttaatctctt atcacggctt acgccgcttt
       61 tattcgtgtt cgctcctttt ttggcacagt caaatatgac tgtttatcct atggctgttt
      121 ccattaatag tcagggtgaa ggcaacgttc gcgtaatatc taaaagtaat gaagttcagt
      181 acatcaaggc gacagtattc cgtatcgata atccatcgac gcctcaggaa aatgaagttg
      241 agattaaatc cggtgatgca aatcatctgg ttgttatgcc acctaaattc gctttaccag
      301 ccggtagtag caaaaccgta cgttttgttg cgatggaacc agagcaaaaa gagaaaaatt
      361 atcgcgttaa atttgaagcg gttcccagta ttgatgacgt tgccacagat aaaaaagatc
      421 tctctatgca gttaacagtt aacttaattt gggggattgt tgttagtgtt ccacctcagc
      481 aacctattgc taagttagaa gtaaatgctg cccaaaaatt agttaatgca gggaatcaac
      541 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
      601 agaccgtaaa taaaaacatc ttccctggtc aggaaagaaa tcttgaaagc atatctggct
      661 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
      721 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
      781 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
      841 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
      901 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
      961 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtgtc
     1021 cccagcgcaa ctggttcaaa gcctggatgc tagcaagatt gttccgctga cagtaacctg
     1081 gggcggtgaa gaaattaaag ctgatgcggc aaccactttt accgcaacca aaatctttgc
     1141 ttccgatgcg ttaactaacg gttctctggc taaaaacctg atgtttgctc aaaccaccaa
     1201 aggtgttctg gagacaggca tctaccgtgg tgtagtaagc atttatctgt ctcaggatat
     1261 ctaatccaga taacagaatt aacacgtaac aaagggaggg gtagccctcc cttattttaa
     1321 ctgttttagt atttgttatg gatttcagga ttgccatgga taaaaaatta ctggctcttt
     1381 tgatcctggc gagtctcagc ccggcagagg cgacattaac caaaattccc gcagggtttg
     1441 aggttattgc tcagggacag caggagtata tcgaggttta tttttcaggg aaaagtctcg
     1501 gtaaatatta tgcaatggtt aatcttgata ccgtaacatt tcttgatcca gcaagtctat
     1561 ataacaagct ggagctggat gtagacgatc agaaaatcgc gcatatagtg aaagaaaaat
     1621 tatcgcagcc gctagctcgc cacggtgaat tggcttgcgg ttatgtacgt actgactcag
     1681 ggtgtggttt tctgaatacc gatacgctgg aaataatcta taatgatgaa gaaagttcgg
     1741 caacgttgtt tattaatccg caatggaatt cagctttcga tgcgaagtca ttatatttaa
     1801 atccagacaa aaatactgtt aatgctttta tacatcagca agacatcaat gttctggcac
     1861 aggatgatta ccaatcgttg tctattcagg gaaacggtgc gctgggaata acagaaaata
     1921 gctatattgg tgcacactgg aatttcaacg gttatgatgc agatgatgtc agtgacagta
     1981 atgctgacgt cagcgatctc tattatcgtt atgatttttt acgtcgttat tatgtgcagg
     2041 cgggtcgcat ggacaaccgc acactattta atgcacaagg cgggaacttt acctttaact
     2101 ttctgccact cggtgcaatt gatgggatgc gtatcgggtc gacactgagc tatttaaacc
     2161 aggcgcaaag ccagcaagga accccagtaa tggttctgct ttcgcgcaat tctcgtgttg
     2221 acgcttatcg taatgagcaa cttttgggat cgttttatct caatagtggt tcgcaattta
     2281 ttgataccag ttcctttccg cctggcagct acagcgtggc attaaaagtc tacgaaaata
     2341 accaactcac ccgcaccgag cttgtgccgt ttaccaaaac cggcggtctg actgacggaa
     2401 atgcgcaatg gttcttacag gcaggtaaaa ctacatcaca ggtttctgat gatgaaagtt
     2461 cagcttatca gctaggagta cgcctgccat tacatccgca atatgagctc tacgcagggc
     2521 tggcgaatgc cgatgatgtg agtgctttcg agttaggtaa taactggacg gcagatttag
     2581 gcggggtggg gaatcttgca atcagcgcca gcgtgttccg taacgatgac ggcggcaaag
     2641 gtgatatgca acaggccaac tggagtaatc cgggatggcc gacgttgggc ttttatcgga
     2701 ccaactctga cggcgatgct tgtacaaccg acagcagaga gagctataac gccttaagct
     2761 gttatgaaag tatttccgcg acggtttcac agaattttgt cggctggaat atgatgctgg
     2821 gttatacccg cacacaaaat aacactgatg atagtttgcg ttgggataaa cagcagagct
     2881 ttgaaaataa ctatcttcgc cagacaacag cgcaaagtat ctctgaaact gtacaactta
     2941 gcgcttcccg cgcttttgtg atgcgtgact ggattttgag tacgtcagtc ggtgttttcc
     3001 atcgtaatga caacggtggc gataacgacg acaacggctt gtacttatcg ttttcgttat
     3061 ctgacacgcc aacgatggat agcaataaca acagccattc aacaaatgtg tctacggatt
     3121 atcgttatag cgaacaggat ggcgatcaaa cgtcatggca gttatcgcat accttttata
     3181 acgattcatt cagccataaa gaacttggcg taaccgtcgg aggcctgaac accgatacca
     3241 taaacagcgc ggttaacggg cgttgggatg ggcaatacgg aaatgtctac gctaccgtat
     3301 ctgatagtta tgaccgtaag aatcatgatc atctttcggc ctttacgggc acttatagct
     3361 ctacgctggc tgtcagtcgc tatggcgtta atttgggagc cagtggtaca gacgatttgc
     3421 tgggtgcggt actggtggat gtgaaaggct tctctgaaca ggatgaagag agtcaggatc
     3481 tgcaactcga agcgcgggtg gcaggcagcc gaactttgca gcttggtcaa agtgacagtg
     3541 tgttgttccc ttatcctgga tttcagtctg gttttgttga ggttaacgac agtagccagg
     3601 gcaatcagca ggggacaaca aacatcatta acggtgcagg gaatcgtgaa ttaatgttgt
     3661 tgcctggcaa gctgcgctat cgcgaagtgt ctgccagctt taactacaac tatatcggtc
     3721 gtttattact gccggcagcg gtgaaaaaat tcccgattgt ggggctgaac agcgccatgt
     3781 tactggtagc tgaagatggc ggatttacac ttgaaattaa cggcagcgaa aaagagctct
     3841 atctgctttc cgggcagcaa ttccttaagt gtccactaag tgttgtaaag aaacgcgcca
     3901 gcattcgtta cagcggagat gttacttgta gtgtggtgac ttattcacaa ttaccggagt
     3961 cgattcaggt tcaggcacag ttgaaacagc ctaaattacg tggaaacgtt cagacggcgc
     4021 aaagggaggt tgcaccatga gaaatcgatt gattgcggcg atattgggct tgtttggcac
     4081 gctcactggc gttcaggcag ctcctgacgt gaccagtgaa attacgtatg atttggcatc
     4141 tggtagagcg gattattact tctggaagga tgaagcgtcg gcaggaaata atggatatat
     4201 gtggtatgaa tgttcgtatc ctgacctcca acaaacctgt acagctaatg gaaatatatc
     4261 gacagtacaa atctatttaa ctgaacaacg cagtgggatg cgttggccgg taaaactcaa
     4321 aggatttaaa acagccattg taagtagcga tgaagcgccg ccaggatgca aggggggcaa
     4381 agggcttcag acgaatctta aggattctaa tagatcttca tgtacagaag atggtcaaca
     4441 ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag agatgaagaa
     4501 tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca gcccggccca
     4561 ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg acgtgttctt
     4621 cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa caggaagcgt
     4681 taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt acgatggttt
     4741 taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac cagctgcagg
     4801 gcgcagagac ggttacttct ctatctatcg tcagggaggg accaccaccg acgagggaga
     4861 acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg
     4921 caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc cggtggtact
     4981 gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg cagtcgataa
     5041 attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag taatctttac
     5101 gccttccttg ccaaccatca attaaccctg caaagcggac aaacgctttt ttctatataa
     5161 acgacgaagc cgtctgataa agcggcttcg tcattacaac ttaagttaaa cgttaatgtt
     5221 gcggtgcggg atcgttatgt gtttcctgcg ccacctcaat cgccggtttg ttctgcaaca
     5281 cggtccagat aaccagcgca cctaacaggt caaatactgc cagaactgcg aacagcgggc
     5341 tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta cttgccagcc
     5401 atgcggacat cccggttaaa ccgttcgccg tcgccacttc gttacgaccg aacacgtcag
     5461 aagagagcgt aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg atacacagca
     5521 gcataattgc gacatacggg ttggtgaaca gaccgatcat acccgggcca atcatcagca
     5581 cagcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca aaccaacgct
     5641 ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac agcatcggca
     5701 tccaggcgaa catcgcgatc tctttcaggt taaagccgta aactttaaac atgaacagcg
     5761 ggatccacgc gttaaaagta ccccaggccg gttctgccag gaaacgcggc agcgcgatac
     5821 cccagaactg acggttacgc aggatctgac caacggacat tttcttcgcc gtgctcacct
     5881 ggtgctgggc ttcctgacca ttaataatat agtcgcgttc ttcatcggtc agatgcttct
     5941 ggtcgcgcgg atgtttatag aaaatcagcc atgccatcgc ccagataaag ctcaatgcac
     6001 cggagatgat aaatgccatc tgccagctgt gcattacgat tgcccatacc accagcggcg
     6061 gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta acctaccgcg atggaacgct
     6121 ctttcgccgg aaaccattcg gagctggctt tcagacccgc cgggatcatc gctgcttccg
     6181 cggcaccgac tgcaccacga gcaatcgcca ggccacccca gctaccagcc agcgcggttg
     6241 caccacagaa tacggcccac agcacagcaa acattgcata accgattttc gtacccagca
     6301 catccagtac atagcccgct accggttgca tgaccgtata agcagcagaa taggctgcga
     6361 tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt ggcgcagctg
     6421 ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc aagcgtcacc agtgcgatca
     6481 tataccaacg taacccttta attttacgca tgtaaaacct catcgttttg ttatatccgc
     6541 aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc gggtaaccga
     6601 aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc ggcttccgta
     6661 ccggttaacc cgattcatct aacaaatgaa tgaatcggca atttgcattc cgtcgactta
     6721 tgatttgcga cggcagaaag ataacttgtc atacaacttt aaaaggtgag agccatcaca
     6781 aatgtgcgaa tatttgtagg gacattacct gacgacagcg aggccagtac tggcgcggcc
     6841 tgcagcgaga tttaccactt tgagagtgat ttttttaact acgtttattg atctaactca
     6901 cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc gcaccataag
     6961 caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac tgaagatttt
     7021 ctgttagata ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga ccagccgatt
     7081 ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg ttttaaaaac
     7141 ctgtatgaca tctggctgaa aggcgatcac tacaaatggc gcgctatgcg taccaacggt
     7201 gtggctgagc gtctgtgtac tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg
     7261 gcgactgttc cgcacactat cggcaacccg ttataccact ggacgcacct cgaactgcgt
     7321 cgtccgttcg gtatcactgg caaattgctt tctccgtcaa ctgccgatga aatctggaac
     7381 gaatgtaacg aactgctggc gcaggataac ttctccgcgc gcggcatcat gcagcagatg
     7441 aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca tcacgcagag
     7501 atcgccaaag acggctcttt caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc
     7561 tttaacattg aacaggcgac ctttaacgac tacatggcga agctgggcga agtttccgat
     7621 accgacattc gccgctttgc tgacctgcaa actgccttga ccaaacgtct ggatcacttc
     7681 gccgctcacg gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg
     7741 aacgaagcgg aactggacag cattctggcg cgccgtctgg ctggcgaacc cctgagcgag
     7801 cacgaagtgg cacagttcaa aactgcggta ctggtgttcc ttggtgccga atatgcacgt
     7861 cgcggctggg tacagcagta ccacattggc gcgctgcgta ataacaacct gcgtcagttc
     7921 aaactgctgg ggccggatgt aggctttgac tccatcaacg accgtccgat ggcggaagag
     7981 ttgtctaagt tgctgagcaa gcagaatgaa gaaaacctgc tgccgaaaac cattctctac
     8041 tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa
     8101 ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgatca gaaagacggt
     8161 atggaacgtc agatgaccca actggcgcag ctcggtttgc tgagccgctt tgtcggtatg
     8221 ctaactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg ccgcattctg
     8281 tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat caacctgctg
     8341 ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc cattgaactg
     8401 aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat aacgtcgcgg
     8461 tcgctttagc ggatttggct gaaggcacag aagtcagtgt cgataaccaa actgttaggc
     8521 tgcgccagga tgttgctcgt ggacataaat ttgcgttaac gaatatcgca aaaggggcca
     8581 acgtcatcaa atatggcctg ccgattggtt atgcattggc ggatattgcg gcgggggaac
     8641 acgttcacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat cgctatcaac
     8701 ctgattttca ggatctgcct gcgcaagcgg cagatcgtga agtgcagatc tatcgtcgcg
     8761 ctaacggcga tgtcggagtg cgtaatgagc tgtggatcct gccaaccgtg ggctgtgtca
     8821 acggcatcgc gcggcagatc caaaatcgtt tcctgaaaga gaccaacaac gccgaaggta
     8881 ctgacggcgt gttcctcttc agccacacct acggctgctc acagctgggc gacgatcaca
     8941 ttaatacccg caccatgctg caaaacatgg tgcgccaccc gaacgcaggc gcagtgctgg
     9001 tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg ctgggcgata
     9061 tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgag atcgaagccg
     9121 gaatcgagca tttgcatcag ctgtataacg tgatgcgcaa cgataaacgc gagccaggca
     9181 aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt tctggtatta
     9241 ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc ggtactaccg
     9301 tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac cattgccgcg
     9361 acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga cttcaaacag tactttattg
     9421 cccatgatca gccgatctat gaaaacccat cgccggggaa caaagcgggc ggtatcacca
     9481 cgctggaaga caaatcactt ggctgtaccc agaaagcggg ttccagcgtc gtggttgacg
     9541 tgctgcgtta cggcgagcgt ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta
     9601 acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg ctgttcagta
     9661 ctggtcgtgg cacgccgtat ggtggatttg tgccgacggt gaaaatcgcc accaacagtg
     9721 aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagttg atccacggta
     9781 aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgagttt gccaacggta
     9841 agcaaacctg taacgagcgt aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa
     9901 cgctataaag tcgaaatgag ttgttaataa aaacggcgtt tcataatgtg aagcgccgtt
     9961 tgcttttcca tacctgctaa ggatttatca tgaccgcgta ttggctggcc cagggcgtgg
    10021 gtgtcatcgc ctttctgatt ggtatcacaa catttttcaa tcgtgacgaa cgtcgcttca
    10081 aaaagcagct ttcggtctat agcgccgtta ttggcgtaca tttttttctt ctgggtacct
    10141 atcccgccgg tgccagtgcc atccttaatg ccgttcgtac attgattacc ttacgcacgc
    10201 gcagcttatg ggtaatggcg atttttattg tgctgactgg cggaattggc ctcgcgaagt
    10261 tccatcatcc tgtcgaacta ttgccggtta tcggtacgat tgtcagtacc tgggcgctat
    10321 tccgctgtaa agggctgacc atgcgctgcg taatgtggtt ttcaacgtgt tgctgggtga
    10381 ttcacaactt ctgggcgggg tcgataggcg gcacaatgat tgagggtagt tttctgctta
    10441 tgaatggcct gaatatcatt cgtttctggc ggatgcagaa aaggggaatt gatccgttta
    10501 aagtagagaa aacccctccc gccgtagacg aaaggggtta aacaattaat tacgcagggc
    10561 gctatttgcc agacgatcgt cttccgcctg gcaagctgcc gcagtgaaca gcacgtcagt
    10621 tgaagagttc agcgcggttt cgcaagagtc ctgcaatacg ccgataataa agccgacggc
    10681 aaccacctgc atggcgatat cgttcgaaat accgaacata ttacaggcca gtgggatcag
    10741 cagcagagaa ccccccgcca cgccggatgc gccacaggca cacagagaag ccactacgct
    10801 caacagcagc gctgtgggca gatcgaccgg aatacccagc gtattaaccg cagccagcgt
    10861 caacacggta atggtgattg ctgcgcccgc catattgatg gtggctccca gcggaataga
    10921 aacggaatag gtatcgcgat ccagattcag cttttcacac agcgccatat tcaccggaat
    10981 gttcgctgca gagctgcggg tgaagaaggc atacacaccg ctttcgcgca ggcacagcag
    11041 caccagcggg aacgggttac gacgaatttt ccaccacacc agcaatgggt taaccaccag
    11101 cgccaccagt aacatacagc caaccagcac gaccagcagt tgcgcgtagc cccacagtgt
    11161 ggagaaaccg gtggttgcca gggtagaaga aaccagccca aaaataccaa tcggtgcgaa
    11221 gcgaatgacc agtttcacca taaaggtaac ggcattcgac atatcgttaa ccaggttttt
    11281 ggtggtctcg ttaccgtgac gcagtgcgaa gccgaggccg atcgcccaca ccagaatccc
    11341 gatgtagtta cctttcagca gcgcgtcgat ggggttggaa accatgctca ttaccagccc
    11401 gcgcatcact tcgacaatgc ctgacggcgg cgaaatatca cccgcgctac tggataaatg
    11461 cagggtagaa gggaaggcaa agctgaagac tactgcggcc agagcagcag agaaggtgcc
    11521 cagtagatag aggaacaaaa tagggcggat attggttttc tgcccgtgct ggtggttagc
    11581 aatagatgcc attaccagca tcaacaccag gatgggggca acggctttca gtgcgccgac
    11641 gaacaaagta cctaacagac caacagcttc cgccgcgggt tttgagatcc atgccagaag
    11701 aatccccaga acaaggccga ccaggatttg ttttaccagg ctgccatgag ccagacgccg
    11761 gaatagcccc ggtgaacgtt gcgtagtcat ttttcgatcc tttcattgtg ttgtcgcaca
    11821 tccctggtgc gttctgtcga cgcatctttc aggatgtaac aaagtgtttg caaggtcgag
    11881 tataaggaaa gttacagctt cgggaagcga aaaatgctgg attttaaaga gaggtcatat
    11941 tttgcaactt aatggttaca tttatttgac ataaaaataa agccaacgtg cggcttccag
    12001 actgatgaca acatcaaaaa aggactcggt cagtcccctc gccccgtttg gggagagggt
    12061 tagggtgagg ggaacaggcc agcatcggtg cgaaaaatta ccctcacccc gaccctctcc
    12121 ctggaaggga gagggagaaa acggtgctca tgttaatgac agggtacacg gcttaggcag
    12181 attaaaaatt atccaccccg ctgcttatca tgccgataat tcacccaggc gttgataata
    12241 aacgtcatca ccagaatgcc aaacaccacg cccagcgaga cggcgattgg aatatggtag
    12301 aagtcgacaa tcagcatctt gataccgata aacaccagga tcaccgccag gccatatttg
    12361 agcatcgaga aacgctctgc tacgcccgcc agcaggaaat acatcgcacg caggccgagg
    12421 atcgcaaaca ggtttgaggt cagcacaatg aacgggtcag tggtcacggc gaagatagcc
    12481 ggaatgctat ccacggcgaa aatcacgtcg ctcaattcca ccagaatcag taccagcatc
    12541 agcggtgtgg cgtacagcaa tccattctta cgcacaaaga aatgctcgtt gtcgatggtg
    12601 tcggtcatgc gcaaatgacc gcgtagccag cgcaccagcg gcttgtcgcc aatgcctgat
    12661 tcatcttcat gggcgagcgc catcttcacg ccggtaaaca gcaggaaggc accgaagata
    12721 tacaggatcc agtcgaactg cgaaattagc cagctgccag tgaagatcat gatggtacgc
    12781 agaacaatcg ccccgagcac gccatacacc agcacgcggc gttgtaatgc cgccggaaca
    12841 gagaaatagc tgaacaacat cagccagaca aagacgttat cgaccgccag cgatttctca
    12901 atcagataac ctgtgagaaa ggccagtgcc tgtggatcgg caacggcgcg accttcggtt
    12961 tgcaccagat accaccagaa agcggcgtta aacagtaacg acagcgtcac ccagaccagc
    13021 gaccaggccg cagcctgttt catggtcatg gcatgtgccc cacgacgccc ctgcaacaac
    13081 aggtcgatag ccagcataat ggcgacaaca acagcgaatc cgccccatag caacggcgtg
    13141 ccgacagtat tcatagaagt tccttacaca taaaaaaacg gccaacgtcg gaagacgtca
    13201 gccgctgctt tttatgcata gacctcgcct tccggcaagg tctcacttac aacaaaaaag
    13261 gaatacgtct gcgtattcct ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc
    13321 gtaatgacga tcgaccggca atgaagttac tcccctttgc aggtaacaaa gtatgacaga
    13381 ccattcagcc agtcaatggt ctgttacata ccttttacac tgttttactt tggcaattat
    13441 agttttacgc tatctgccgg aaaaatcacc ccagtctggc ggcggatctc ggtcagcagt
    13501 ttggcggtga tgcgactgac cgccagcccc ggatgatcca ccagatgctc atccaccagg
    13561 gtagcgaaca gctctgcttc gtagagcatg gtattaatat gctgcggctg ggtgagatcc
    13621 tgcatttggc tgccacgcgg cacgaagcac actttctggc attcagacag tttttcaatc
    13681 accagcgatc ctgcttcgcc ctgaatctcg ctcgccagga cagaatcact gacttttgag
    13741 tgctgcaagg tgacgctgaa atcaccgtaa tccatcacca ccacgccctg ggcgtcaacg
    13801 ccgcttgcca gcaaactggc ggttgcctgc acgcttttcg gctcaccaaa taacgccacc
    13861 gccgacgcca gacagtaaaa accgatatcc ataattgaac cgttagagaa tgccggatta
    13921 aaggtgttgg gattctcacc atccaggtaa cgttgatacc gcgaggaata ctggcaatag
    13981 ttgaaaaaga ctttccgcag tttgccgact ttcggcagcg cctggcgcaa caaatgaaag
    14041 ttcggcaggc aggcggtttt aaatgcctca aacagcacca cctgattttc ccgcgcacag
    14101 gcaatggcgg catccacttc cgccagattc gacgccagcg gtttctcgca aatcacatga
    14161 attttatggc taaggaaaag ttgtgtctgg gaaaaatgca gggaattcgg gctggcaata
    14221 tacaccgcgt caatggcatc gctttccgcc atcgcttcca gcgaggtaaa cagatgctcg
    14281 acagaaaaat cattggcgaa gtgctgggcc tgttcaaggc tgcgggaata tacggcggtt
    14341 aacttgtatt taccgctctc atgggcggcc tcgacgaact ggcgagtgat ccagttcgta
    14401 ccaatcacag cgaaacgtat cataacgcgt ggatactcca taaagggatt ctgtcgtcaa
    14461 tgtagcacgc ctttttcatc ttcgacgtgc gcagatacgc attatttctg cgccggacag
    14521 ccatgctcac taaacggccc aatcatgacc gaaggcccga gatatttcgg ctggcgatgg
    14581 aggattttgg cgtcaatcac ggcgctgaca cgagcgagtt tctcccgcag ttgagtataa
    14641 aaaccgtgct tcatattgag gtctggcgcg ttaaaaccga tagcgtcgat gccgtactgt
    14701 tttgccagcc agatggcgcg ttgattatga aattcttgcg agataatggt gatatggttt
    14761 tcaccaaaaa cctttttggc acgcactacc gaatccagcg ttgagaatcc ggcatagtcg
    14821 cagaagatca ctttcgccgg tacgccttta gcgatcaaag cctgctgcat acctgaggct
    14881 tcatcgtaat ttttacgtcc gttatcgcca cttaccagta gccatttcac ttttccggcg
    14941 tggtacaacg ctgctgcggt atcaatacgt cgggtaaagt agcgattacc cggcctcgcc
    15001 cccagcaata aacccacgtt acgcgccgga actgcgttga catcgctcca ggtcagctgc
    15061 ttgctagcgt tgaccataac gcgatcggca ataaaaatgg tcgctcctgc tataagcaac
    15121 aataaacagc caatcttaag agtccggcga gaaaaacaaa tacggagaag aaggcggggc
    15181 tgcatggtcg ttatccttaa cgaaaggggg gaggtcgatt ttaggcgcta aagaaagaaa
    15241 agataacgcc tgacatcatt ttcagatttt gtcctgccca agcgacaact gggttagcca
    15301 cagtcgggtg tcgaactcaa gctggtggta ctgcggctcc atatggcagc acaattgata
    15361 gaacgctttg ttatgctctt tctccttcag gtgcgccagt tcgtgcacca cgatcatacg
    15421 taaaaacggt tccggcgcgt tgcgaaacac agtagcgacg cggatctctg cttttgcttt
    15481 cagcttgccg ccctgcacac gagaaacagc ggtatgtaac ccgagcgcgt tttttagcac
    15541 atggatctta ttgtcataca tcactttatt gatcggcggg gcattgcgca gaaactgatt
    15601 tttcagatcc tgagtatatt gccagagggc tttatcggtg gcgtagtcgt gtgttcccgg
    15661 atagcgtttt gccagcacat cgcccagacg ctgttcgtta atcagtgttc gcacctggga
    15721 aagtagctgc tcggggtaac cctgaagata agtaagattg ctcataaccg cccacgaaaa
    15781 agagaaaaag ggtatactca cgcacccttt tcaggggcaa cgtcgaaatt ttatcattca
    15841 ggagggccga tgagccactt agacaacggt ttccgttcac tgacactaca acgttttccg
    15901 gcgacggatg acgttaaccc gctacaggcg tgggaagcgg cggatgaata tttgctgcaa
    15961 cagttggacg acacagaaat ccgcggcccg gtgttgatcc tgaatgatgc ctttggtgcg
    16021 ttaagctgcg cgctggcgga acataagccg tacagcattg gcgactcata catcagtgaa
    16081 ctggcgacgc gcgagaattt acgcctcaac gggattgatg agtcgagcgt gaagtttctc
    16141 gacagcaccg ccgactaccc gcaacagccg ggtgtggtgc tgatcaaagt gccgaaaaca
    16201 ctggcattgc tggaacagca actgcgtgcg ctgcgcaaag tggtcacgtc ggatacacgt
    16261 attattgccg gtgccaaagc ccgtgacatt cacacttcca cgctggagct gttcgagaaa
    16321 gtgctcggcc cgaccaccac tacgctggca tggaaaaaag cgcgcctgat taattgcact
    16381 ttcaatgaac cgccgctggc cgatgcgccg cagaccgtta gctggaagct ggaagatact
    16441 gactggacta tccacaacca tgcgaatgtc ttctcccgca ccgggctgga tattggcgca
    16501 cgcttcttta tgcaacatct gccagagaat ctcgaaggtg agattgtcga tctcggttgc
    16561 ggtaatggcg ttattggtct gacgctgctt gataaaaacc ctcaggcgaa agtggtgttt
    16621 gtcgatgaat cgccgatggc ggttgcttcc agccgtttga acgttgaaac caacatgcca
    16681 gaggcgttag atcgcagcga gtttatgatc aacaacgcgc tctccggcgt ggagcctttc
    16741 cgctttaatg ctgtgctctg caacccgccg tttcaccaac aacatgcgct gaccgataac
    16801 gtcgcctggg agatgttcca ccatgcccgc cgctgcctga aaatcaacgg cgagctgtat
    16861 attgttgcca accgtcacct ggattacttc cataaactga agaagatttt cggcaactgc
    16921 accaccatcg ccacgaataa taaatttgtg gtgctgaaaa cagtgaagct ggggcgtcgt
    16981 cggtaagatt gggagttacc tgccggatgc ggcgttaacg ccttatccgg cctacgttca
    17041 gcacgttacc cgtaggccgg ataagacgcg gcaagcgtcg catccggcac ttcaacgcac
    17101 tttttaaatc tccagcgcca gccgtgtccc cttcgcgatc gcccgtcgtg cgtccagctc
    17161 catagccaca tcgcagccgc caattaaatg cacagttttc ccgctatcaa tcagcggttg
    17221 cgccagcgcg cggtttggct cttgccctgc gcagatcacc acattgtcca ctgctaatac
    17281 ctgcgtttcg ccgttgatca ccacatgcag cccgtcatcg tcaatcttct gataacttac
    17341 gcctgggatc attttcacac cccgcgagag cagggtggtg cgatggatcc agccggtggt
    17401 tttgcctaac ccctgtcccg gtttgctggc tttgcgctgg agcatcacaa tttgccgtgg
    17461 gctacgcggg atctgcattc cctgcgggct taagccacca gcctgttgta ggctgctgtc
    17521 gatcccccac tcattacaga acccggcgat attctggctg gtggattcgc ccggctgact
    17581 taaatacatc gccgtgtcaa agccaatccc accacaaccg atgatggcaa ctttgttgcc
    17641 caccggcgct ttgtcgcgca gtacatcgag ataactcaat accttcagat gatcgatccc
    17701 gtcgataggc ggaacgcgtg gcacgatccc actggcgagg atcgtttcat cgaacgcctg
    17761 taactgatcc gccgtcacgg tgtgattgag tttcagcgtc acgcccgtca cttcgatcat
    17821 ccggtggtaa tagcgcagcg tttcgtaaaa ctcctctttg ccggggatct gtttggcgat
    17881 attaaactgc ccgccgatct cgctgtgggc atcaaacaat gttacctgat gcccacgcgc
    17941 cgccgcgtta atggcaaacg ccagcccagc aggtcccgca ccgaccaccg ccagattttt
    18001 tttctgcacg gcaggaagga ttggcatttt ggtttcgtgg caggcgcgag gattcaccag
    18061 gcacgaggtg actttgccaa cgaagatctg atcgaggcag gcctgattgc agccaataca
    18121 agtgttgatc tcatcggctc gccctgattg cgcttttgac agcagttccg catcagcaag
    18181 aaatggtcgc gccatcgata ccatatcggc atcgccgcgc gagagaatat cctcggcaac
    18241 ctgcggatcg ttaatccggt tggtggtcac cagcggcagc gagacgtgac ctttcagttt
    18301 gcgcgtgacc cagctaaatg caccgcgcgg cacgggcgtg gcgatggtcg gaatacgcgc
    18361 ttcatgccag ccaatgccag tattaataat ggttgcgcca gccgcttcga tggcctgcgc
    18421 cagttctacc gtttcggcaa aagtgccgcc gccttcgacc aggtcgagca tcgacaaacg
    18481 gtagataata atgaagtcat tgccgacgcg ttcgcgcacc gcactcacca cttcaacggc
    18541 aaaccgcatc cggttgtggt aatcgccgcc ccactggtca ctacgctgat tggtgcgcag
    18601 cgtcagaaat tcgttgatca aatacccttc ggaacccatc acctctacac cgtcgtatcc
    18661 tgcctcccgc gccagttgcg cgcagcgggc gaaatcgtcg atcagttgca ggatctcttc
    18721 atgggtgagt tcgtgaggaa cgaaacggtt gatgggggcc tgaaatgcgg agggagcgac
    18781 cagatgcggt tggtagctgt agcgcccggt atgcaaaatt tgtaaggcaa ttttgccgcc
    18841 ttcctgatgt acggcttcgg taatggtgcg atggtgtggg atctggctgg cgtcattgag
    18901 cattgcgcca ccttccatgc cgacgcctgt taaatctggt gcaatgccgc cgctaacaat
    18961 cagcgccacg ccgtgacggg cgcgttcggc ataaaacgca gccagccgtt cagcaccgtc
    19021 cgggtattcc tccagcccgg tgtgcattga acccatcaac acgcggtttt ttaacgtggt
    19081 aaaacctaaa tccagcgggg cgaacagcga cgggtagctc ataaaattgt ccagtatgta
    19141 aaataattgt tatgtggtcg gatgagttct aatttagccg tcgccggaga aaagggaaaa
    19201 ggggagtgtg gggtttgtga tgggattcaa aaataatacg cccattggaa atgccggatg
    19261 cgacgcttgt cgcgttttat ccaggttacg gaattgcaca tgtagggcgg ataaggcgtc
    19321 cacgccgcat ccgccagtgg cgcggtgcag atgccggatg cgacgcttgt cgcgtcttat
    19381 ccgacctacg ggaacgcaca tgtagggcgg ataaggcgtt cacgccgcat ccgccagtgg
    19441 cgcggtgcag ttgccggatg caacgcttgc cgcgttttat ccggccagga ggattgcaca
    19501 tgtagggcgg ataaggcgtt tacgccgcat ccgccagcag ttgcaaatta ttgcttacgg
    19561 aaaaaatcgt tatacaacat aaacaaatgc gcggcactcc aggagaaatt cggtgcccct
    19621 tgctgcgcgc cagtgagcgg gttgtaattc tcctgaattg ggccatcggc ggttaatcct
    19681 ttggcgtgcc ggaagaacgt atccgccagt ttcagggcat catcgcgata accgtaacgc
    19741 tccatccctt tcagaccaaa ccagaactga tccacccata cgcgcccgcg ccagtagata
    19801 tccgcgccaa aagccgggtt ggttaacgcc gccgttccca gcgggacaaa ggtgttgaac
    19861 tctttaggat cgagcatcac cttcaccacc gcgtcggcat tggcctgcgt tgccgcaccg
    19921 ttaaacagcg gcgaccagcc ttccggccct ttaccgcgct caacaatcgg tttgcccgcg
    19981 cagccgttcg ccagcggttt atcttcaata cgcacgtcat agtagaactg ggtagtcggg
    20041 tcgaacatac aggtgttgat gtagtccgcg agctgctgtg ccaactggcg atagcgtttg
    20101 gcctcttctg gcttaccgag gatggttgcc atctccgcca gataatggtt atcgctgtac
    20161 atatagctgg cctgatccac cgactcctgc aatagcgagt agcccagcaa cgttccgtcc
    20221 tgactgcggt tttcggcgaa tttcaccgtc cagtcgctac gtttgccgcc attagcgaca
    20281 tatttatcca actgttcttt gtcgataaac ccaaagacgg cggcatcatc acgccccgat
    20341 tcccacgatg cagcaacctg tgccggaatt tccagactgt cgtactggcc tttctccacc
    20401 acgcgggcgt agttgttcag cccagactgc gtctcttctt tatcgccttt tttcaccgta
    20461 aacagcatct cgccgctctc agtgttgtgg gctttgtcgc gggtcgcgcc atattccggc
    20521 acgccgttgc cgttgtgatc gcggttacgt aaccaccagt cgtgataggc caccagtttc
    20581 gggtacatct ctgccagcca ggttttatcc tgggtgacgt tgtacacttc catcaccgac
    20641 caggcggcaa ggctgggttt ggtattacgt tcgttccagt tgccgccatc gccaccacgc
    20701 tcggggctaa ggttccacgc tatcaggtcg gggacaaagc ccacatcctg cggacgcacg
    20761 ctatcgccag gctggatctg ccaggagaag accgcgcgga tattctcttt ggcgatgtcc
    20821 ggattgaaat gcgccatcgc aaacgcctgc ttccaggtat cccacggcca ggtctgattg
    20881 ccggagaacc agcgcccggt caccgacggt gtaacggtgt taaatttcac cgcaccgccc
    20941 ggtgagcgcc agttaccgtt gagcgtttcg atcgctttca ctgcgacgcg cgtctgttcc
    21001 ggcgtcgcat ccggattggt taaccctttc ttcaggtatt cttcccagcg ttgctgcgag
    21061 gcggtgagat aaaacgccgg acgcgccaga atatcgcgga tctgcatttg ctctttgcta
    21121 acttcctgag cggtcagcag atgggaatag gtggtgtaga gcgtggtcga accgttgata
    21181 tgcgccttac tggtaaagcg attaccgttg atttcagtct gcaccggcag ggatttatgc
    21241 acctgatatt ccgattcgcc ggaggtcagc agatcccagg tggcgcgcac tttgccaaag
    21301 gtgactttca ggccatcacg ggtggcgctg attttgcgct gatagacagg gtattcgcca
    21361 gcaatggttt tatcggaaag cggtttccct tctttcgctt ccagtttttc cagcagttcg
    21421 ccgtcccaca ccagatccag cggtttattg ctggtgattt tggtttccag tagtgacgtg
    21481 cgcggcgtgg cgaagcgcag agtcatttcg acttgcacat cttttgctgt cagtttttgc
    21541 accagcgcac cgggaatact gtatgcctcc agcgtaaagt cgactttctt gccgtcctgc
    21601 cagacggtca ggcggtcgaa attgctagcc ataaagttga tgtactcttc cgtcagcagc
    21661 gcaacgcccg gaaagccgcc catggtgtta gggccgtctg gcaacagatg accatgccag
    21721 gctccgagat cgaaaaacgg attaaaacgc tggtgatcgt cgtaatcgta atccttcatg
    21781 tactgcggcg cgccagtacg gttaatcacg tttttataat tgtcggcgtt agcggcatgg
    21841 gcggaaaaac ttatcagcag agcgcaggtt actggcgtta aaatagtttt tattttcatg
    21901 aaattacagt ccttattacc agtagaaata ttgcatcagg aagatttggt cgctgggatc
    21961 gtgagaggca tttttcatgt agaagcggct atcaaaagcc gtggcgaaac gtccgccgcc
    22021 gtattcgtac cagataccaa actgcatgaa agaggtggtt tcatcgtcag cgtggtcagg
    22081 gcgatgtttg gcgtaggtgt aagcggtgga gagataaacg ccctttaacg cttcatacat
    22141 ggcgctcacc atcatcccac tttcacttcc cggtacgtcc agcccgtcgg cgcgtccggt
    22201 atggtgccag gcgcgagcgg caaagttagg agttagcagg cccgtgagat agagctgtcc
    22261 tgtgccgtcg gtgatttcca gaccgttcat ccaggtgatg ccttcggcaa ggtcatactg
    22321 gatgtaaccg ttcatcattg ccggataggt gtatttgtcg tcatagcgat cgtacttgcc
    22381 aaaatgtagc caggctttgc tttcgtcatg atgtccggca ggtgtggcgg tgacgctata
    22441 acgaaagttg ccgcttaagt tctgaacctt cagcgcggtc ataatgtcgc gggtattggg
    22501 aatgacgtag cccagatcgg gtgtgaagtc gccccaccat tgcaggtcat ccagtgagga
    22561 atcctgacgc agactgagca tgatttcggt gccatcattg gtacgataac cgccatagaa
    22621 acggttaatc ccaccttcga aaccacccca gctatggtca ggcacccagg cattaccgtc
    22681 gtggtcggcc tgcacggtcc agccttcgcc gtaaatcagg ccgaaccagg agccgtactt
    22741 catctccagg ccaccttcaa tataggtgcc gtcgctgtat ttatgtttat tctcaccctc
    22801 taaatccata tatccaccaa cccctaattc gccgtaaaaa cgaaatgaag gagaagacat
    22861 cgaggtaatg gcgggcgttt ttgtgtcgtc atgttctgcc ggacgtactt cttctcctgc
    22921 cgcagaaaag gcgtagcaaa acggcagagc aagtaatgct ctgcatggtg cagtgattaa
    22981 tttcattgtg ttaatccatg agctaaagaa aaaatacagc cttttatcgt tgtaattata
    23041 aaaatataaa ggcagaaact attttccccc tccccatcca ggaagaggga aaatgcctgc
    23101 tcatttttta ttagtctgct caatcaacat tgctggctgg cgtcgcttca atatggcgct
    23161 cttcagccgt taatccattt atatatttac tgtatttcca ccacgcaaag ccaaggaaga
    23221 taacaatacc gccgacgtta taaaaaatga tggtcagaat attcgcgcca gtcgggaatg
    23281 tcgaagcgac aaaccctacg gcaaatatcg caatcagcat tgaaacaacg ataataccgg
    23341 tacggcggga acccatacgg aaatcacgtg gcaggtgatc taatttggcg cgtaaattca
    23401 ggtaagccag catgatgaat aacggcggaa gcatggacgc tgcggcggtc atattaataa
    23461 tggtattcat cagatcctgc acggtattgg aaccgagcat cgggataatc atcagcggga
    23521 tgacaatcag gaactggatc cacgctgcgc gcgccggaac gccgttttcg ttaagttcga
    23581 cggttttctt accaaagatg ccttccggga tttcggagaa gaaaattttc accggcgttg
    23641 cggtccacat cagcagggaa ccgaacattg cggtaaagga caccagaccg acaaagcgat
    23701 tcatcaacgc ttccggcaga ccaaaatacg ccgccatgcc gtggaatacc tgcaccgatc
    23761 cgccggtaaa tttcaactct ttgctgctga cgaagacgtt aatcagcacc gaggagacgg
    23821 aatacagtac gccgataaaa atcccggcga ggatgatcac tttaacgaac gatttcgaac
    23881 cgcctttgac gtcgttaacg tacaccgcga cggactccgc gccacctgcg gccataaaga
    23941 tccaggtggt aacgccgagg aacgcccagt tgaagttcgg gatcatcgcg tcaacggtga
    24001 tggggtcagc aggctgtacg ccgccaacca gtgccgtacc tgccagtaaa atgtaggaga
    24061 gcgttaacag cagcatcagc gttgaagtga cggaggtgat tggccccagc attttcgccc
    24121 cgttggtgga aacccaggtg gagaaggcga acagcaccat actgataatg gtggtggcaa
    24181 ccggcgtcat aatatattcg tatccgagga aggcatacga agcataagca ataacgcgcg
    24241 gcaacagcga ggtgaaaaag aacaggttta cgaaccagta ggtataggca gtaataaatg
    24301 cccaacgtcc gcccagcgaa ctttttaccc acgcgtagac accggcttct gagtttttat
    24361 ttaacgaaac aaattctgcg atgatcagac agaagggaat aaaataaaaa atcgtcgcga
    24421 ggaaaaacat cggtgccgag gccagtccaa gctcaatatt attattgata acgttgttaa
    24481 agctgtaaac ggcggcaaaa gtcagcgaga gcaagccgaa tttgccgatt gtattacgtt
    24541 tggtatcaga cataacacat ctccgaaaat gagtagggta gcggtggcgg caaactcctt
    24601 ttgccgccgt agttgttatt tgttatgaaa ataaccatct tcgatggtga ctttgagaac
    24661 cacttttttg accgcgttat tgcagataaa ccgatatgct tcatggatat cgcaaataac
    24721 gatttgccct tcgtggacct ctacggtttc tccgcagcct tttaaatatt cgcggtcagt
    24781 ttcgtcacga taatattcca ctacctgtaa tgtctctttc ggcgcatatt caattttttg
    24841 ctgcccttgc aggtagtaat gcacttcaaa atagcgtcga tgcccggtaa atagcgcatc
    24901 ggtcgcggag tctgtctcca cgcggtaagt caatgagtca ccgatggagt gggcgacgcc
    24961 aggctgaatg ttgtcgatat tttcaatcgc ttcaacgcag cgttgccact tcttaccaga
    25021 ggcgtaaatc tggcggaact gttctaagtt atcgatgatc ctcattgctt attctcgctg
    25081 tgcaaatttg tggaaaagaa ccctgcgccg aactcatacg acgccaggct ttgcgcggta
    25141 gcttctccgc cagaaaccgg cagcaacgta aagccgtagc tgaagtcacg gaaccagacg
    25201 cgccaggagt ccagcacctc gctgccccag gagttggagc cgaggccaag cagctggtgg
    25261 tcgagattca gggtgatgtc atcactgcgc tgcagctcgt tacagtgctg ggcagcgtgg
    25321 atgttttcct ggggatagtg ccaggcgctg aagttaattg ggcgctgcgg aaccaccagc
    25381 agaccgttgc cgtggcggtt agtcagtgcc gtccagcgga catgctgacg gttgccgttg
    25441 ttctgcggga agggatagtt ctcgaacatg gcatcgacgg tgctgcgcca gatatcgatg
    25501 atgttagcct gctggctgtc ggcgtagttt tcgcccggtc cacgaccgta atacgccacc
    25561 tgatcgtatt cgccgttaat ccccatggtg aaaccgatgc acggaatgat gtgcggatag
    25621 tcgccgtaac gctcgccgga aagcgccacg ttaacctggc catcggcagc gatgcgccag
    25681 atgtaggtgc agcgcatccc gaagtcaaac accggcggtg ctataaccgt gcggctgatg
    25741 atcaacactt caccatcgct ctgttctacg gcaaagtcgc gcagatgttc ctgcatgatc
    25801 tgcaaatgat tcggctgcca cagcccttcg tactcctgct tgtggttgtc gatcatcggc
    25861 ttgaagaagt tgatctttgg ctcgcgagtc agcagcgact cgccatttac ctgccaggat
    25921 gtcggtttgc cactcatttt tgagaaggtg atcgcgaagt tgtagccgcg aacggtgcag
    25981 ctcaaacgat cgtcttccag cgtcagcgga cgcgcattat ttggtgcgaa aggcactggc
    26041 tgcgcggtgt tttccttcag cgggaactga taagtggcga tagaatgccc ggcttcgctg
    26101 tagcgggtgc gggaatcttt ggtcaccgta atgttgagga acgcttcgcg ggcgtccagc
    26161 tgcggcagcg tgatctgcaa gggggcttcg ctgttcggcg caacgtcgcg cagtttaatc
    26221 tgctgcgttg cgagcgtttc accttcggcg cgcacctctg cgtgcagggt gtagtcatca
    26281 agcgtggtaa accacagttt attttcgact ttcagctcgc cgcgagtcag atccagcgcg
    26341 tggattttta ccggcgcgat aacctgtttg tactctttca ggcctggtcc cggcgtctga
    26401 tcggaataga tcaaaccatc aagacagaag ttatagttgt tgggatagtc gccgtagtcg
    26461 ccgccgaatt tataccagac attgccgttg tcatcctgcg cctggatccc gtggtcgcac
    26521 cattcccaga cataatgtcc ctgaatacaa tcgtgcttat agaagacgtt ctggtactcc
    26581 gtcagcccgc ccggtccgtt ccccatcgca tgagcatatt cacagatgat gcgcggcttc
    26641 ggatgcgggt attcaccaaa ctcattcatc agcggcacgc gggtgtacat ggtggaaata
    26701 atatcgacca cttcagcatc gcgatcttct tcgtaatgca ccagtcgcgt gtcatccagc
    26761 gctttagccg catggtacat cgcgcggatg ttacagccat agccggattc attgcccagc
    26821 gaccagatga tgatcgacgg atggtttttc tgcgcgtgaa tatggcgaac aatgcgctcg
    26881 acgtagactt tttcccactg cggatcgtcg gtaatacggc tgatatcgcc gacattagca
    26941 aagccgtgcg attcgacgtc ggtttccgcc atcacaaaca agccgtagat atcacacagt
    27001 tcgtaaaaac gcggatcgtt cgggtagtga gcggtacgca cggagttgat attgtgctgc
    27061 ttcatcaact ggagatcttt ctcgacgcga tccattccaa cggcgcggcc tttgcgatga
    27121 tcgttgtcgt gacggttgac gccgtgcagc atcacataac ggttattgat ccagaacaga
    27181 ccgtcgcgca ctttgatatc acggaagcca acgcgttgtg gtaccacttc cagaacgttg
    27241 ccgtcggcgt ctttcagcgt catgaccaga tggtaaagat aaggggattc tgctgaccat
    27301 tgctggggct gttcgacagt aaaagcaaag ctggcgctgg tcagtttttc aattgccaaa
    27361 tgatcaatgg tgctgctgtg caccacgcgt tcgccatcaa acagggtata ttccagcgtc
    27421 gttacgacag gggaggctgc gagattttcc agcaccactt cgcaggaaag cgtggcatcg
    27481 caataggctt cgtcaaagtc ggtacgcaca gtgaaatcgt taatatgcgt taggtgtttt
    27541 ccgaccagat aaacatcgcg gaagatcccc gccgaccacc acatatcctg gtcttccacg
    27601 taggtagagt ccgcccactg catcacgcgc acacacaaca ggttgtcgcc ggttttaacc
    27661 atcgcgctga tgtcaaactc tgcggtcagg cgactgccct tgctgaaacc cacatactga
    27721 ccgttaacat agacttcaaa ataggtttcg acgccgtcaa atttaatcag cgtctgttta
    27781 ccctgccagc cgtcgctgag ggtgaaaata cgttgatagg caccggttgg gttatcgctg
    27841 gggacaaacg gcacatcgat ggggaacgga aaaccttcgt cggtatattg cagtttgccg
    27901 tgaccttcca tttgccacat ggcggggacg gtaatatgcc cccagtcagc cattaactca
    27961 gaggtgaagg cttcaggcac ttgcagcggg tggtcaaaaa aatggaaatt ccactgaccg
    28021 cttaagggca gaaacaggct gctggtttcg cgggcaaagg tacgcgcctg tgcaacagaa
    28081 tcatatgaaa aaaagtacgc acgcggcgca agtcggtttt cgtgggtgag ctgaatgttt
    28141 tcccagcgat tcatagggcc tcccggtaga gagaaatcgg aaccattcat ggtgatggta
    28201 gtttgtgtaa ttgctgggga gtttagtaaa atttttacta aaaagtagca gttaaatgca
    28261 tttactttaa ctggatcacg aaaataaacg gcagcactgt ccggcaaaaa cgactgccgg
    28321 acagtgtgta aggggattta acgggtcgtg ccgcgcagtt ttaatttgct gggaacgaag
    28381 actaacagcg gcagcgcgcg accatcgcgg gctttttcat acaccaggtt aacgccctga
    28441 cttcccatca tctcggaatg gatgcgcacg gtggagagcg gcggaaaggt aaatcgcgcg
    28501 gtggggatat cgttaacgct gataagcgaa atatcctgtg ggatgttcag gccacgttca
    28561 tgaattgccc gcagtacgcc gatagcaatg gaatcggaag caacaaacag tgccttcgga
    28621 tagtcttccc gcgacagcat ctgttttgcc agttcatagc ccgacgaact ggaaaaaccg
    28681 ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc ggccatattc cacaaaggcg
    28741 acctcacgaa tatccgcctt gccaggctca tcttcaccgc caataaaacc aatgcgatta
    28801 acgccctggt tgatatagaa gtcgatgatt tctttactga tgcgtgccag atcgatatcc
    28861 accgcatcgt aaccgctgcc gggttcgtga aagtcgataa aacagatatt gtcggtcaac
    28921 gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga caattaaaat accggtgacg
    28981 tttttaatgt ctggtaatcc gctgtgttca taacagttgg tgagctcgat gcccagcttt
    29041 tcgcactggg tttcaatgcc gtggcggatc gccagatagt aaggatcgtt gatctccagc
    29101 tcctgctggt agctgtagat agccagaata tggtgttggt tgactgcgcc tgtctgtagt
    29161 ttacgggcgc tactggtctt gtactccagc ttttcggcga tctcgagaat gcgatgtttc
    29221 gtctcttctt tcacattcaa tgtcggatcg tcatttaaga ccctggatac tgtcgccagg
    29281 gatacgccag cttcgattgc gatgtctttt agtgttgcca tgtttacctt ttttactttc
    29341 cctgatgtgc gcttccattg tagaggaagt gcttattggg tcacctgttt tcagtaaata
    29401 aagcgcgttt ctttgacgct gtaacggtaa cacagcggct cacgaatgtg atcacgcctg
    29461 cattctccat ttttagccta caggattgag aaaaactttt gctatagttt agtaaaattt
    29521 tactcgaagg gaggaagcaa tggaaaccgt ggcttacgct gattttgcac gtctggaaat
    29581 gcgcgtcgga aagattgtgg aagtgaaacg ccatgaaaac gccgacaagc tgtacatcgt
    29641 acaggttgat gtgggggaaa aaacactgca aaccgtgacc agcctggtgc cgtactacag
    29701 cgaagaagag ttgatgggaa aaacggtggt ggtcttatgc aatctgcaaa aagcgaaaat
    29761 gcgtggtgaa acgtcggaat gcatgctgtt gtgtgcggaa accgatgacg gcagtgaaag
    29821 cgtgctgtta acgccggagc ggatgatgcc tgcgggcgta cgtatcgtat aaaaagatcg
    29881 gatggcgacg tcgtatcgcc atccgatttg atactacgct tcttcgacac ttactcgcat
    29941 ggccgccagc gccttacgcg ccgctttgat caccagttca cactgttcaa tggtcagtgt
    30001 cagtggcggt tcaatgcgga tcgttttggc gttattgagc gttccggcca ccagtacgcg
    30061 ctggcggaac atctcgctgg caaagttata gccgatttcg ttatcaacaa actcaatcgc
    30121 catcaacatc cctttaccac gtgcttcctg caccagatcg ggatattccc gcgccagttg
    30181 acggaaaccg tccagcaaca tatcgccttt ttgttcagcc tgagcaggta agttctgctc
    30241 cagcaacaca ttgatggtcg ccagcgccgc cgcacaggcc agcgggttgc cgccaaaggt
    30301 ggtggtatgc aggaatgggt tgtcgaacag aactgaaaac acctcttcag tggcgatggt
    30361 cgcgccaatc ggcatcacgc cgccgccgag cgctttggca aggcagagga tatccggctg
    30421 tacgttctca tgctcgcagg cgaacatctt gcccgtgcgc cccatgcccg tttgtacttc
    30481 atcgaggatc atcagtgcgc cgaactcatc gcatagctta cgtacggcgg tgagatagcc
    30541 cggcggcggc agaattacgc caccttcacc ctgaatcggt tcgaggatca ccgcagccac
    30601 atcatcaccg gtttttttgc actcgttaag agccgtgcgc atggcttcga tattgccaaa
    30661 cggcacatga cggaaccctg gcagcagtgg cataaacggt ttgcggaagg tcgatttcgc
    30721 cgtggccgac agcgcgccaa gtgatttacc gtggaacgcg ccgctggtgg caataaaagt
    30781 aaatttgccg cgcggcgact gataagcctt cgccagcttc agcgccgctt caacggactc
    30841 ggtgccgcta ttacagaaga agctgtattt cagtttacct ggcgttagcg cagcaagggt
    30901 tttcgccagc atagcccgta acggatcgag tagctcctgg ctgtgcagtg gttgtttcgc
    30961 aagttgattc tgtacggcgg aaaccacaac tggattacgg tgccccacgt tgaaaattcc
    31021 aaaacctccc aggcagtcga taaactcctg tccctgggtg tcgacaagcg tatttaaacc
    31081 tcccgcttgc cactctacgg ctccgtaatc cccgccggcg gtaacagatt tgcgatactc
    31141 taaaaacccc ggattgacat gctctttgaa gtattcaatc acctctcggt taagtgcttt
    31201 catctcctca tgatccagcg ttcgcttttc aatgagattc agggcgtggg cgctgcacgc
    31261 taaagccgat gcgctcgaag gtaacctgtt caaaatatgc tccggaggct cgcgtatcac
    31321 atgataccga tttaagtatt gcagggattg cgccactccg gctgcgatcg cgaaaatcgg
    31381 gataaacaca aatgtggtta tcatttgcgc aatatttaat gctatttgtt aacatccgtt
    31441 gttattttgc acggtttttg tcgggtatgg atttgataat tgctgcgcaa atgcacgttt
    31501 tcagggcaat aattgcctga tttttgggca agataattaa cgttttcgta tagtaacaga
    31561 gtgaaagtat tttaaatcaa ggaattagat ttatgcttta atcgcaaatt gcgatctaaa
    31621 tcaaattaat cggttaaaga taaccgcagc ggggccgaca taaactctga caagaagtta
    31681 acaaccatat aacctgcaca ggacgcgaac atgtcttctc atccgtatgt cacccagcaa
    31741 aataccccgc tggcgaacga caccactctg atgtccacta ccgatctgca aagctatatc
    31801 actcatgcta atgacacttt tgtgcaggtg agcggctata ccttgcaaga gttacagggg
    31861 cagccgcaca acatggtgcg tcacccggat atgccaaaag cggcgtttgc ggatatgtgg
    31921 ttcaccctga aaaaagggga gccctggagc ggcatcgtga aaaatcgccg caaaaatggt
    31981 gaccattatt gggtgcgggc caatgcggta ccgatggtgc gcgagggaaa aatcagtggc
    32041 tatatgtcga ttcgcacccg ggcgacggat gaagagatcg cggcggtaga gccgctgtac
    32101 aaagcgttga acgccggacg taccagtaag cgtattcata aaggcctggt ggtgcgtaaa
    32161 ggctggctgg gtaaactgcc ttcattaccg cttcgctggc gggcgcgtgg cgtgatgacc
    32221 ctgatgttta tcttgctgac ggccatgcta tggtttgttg ctgccccggt ggtgacgtat
    32281 ttcctctgta cgttagtggt attgttggca agcgcttgtt ttgaatggca gattgtgcgc
    32341 ccgatagaaa atgtcgcccg tcaggcgctg aaggtggcga ccggagagcg taatagtgtt
    32401 gagcatctga atcgcagcga tgagctgggg ctgacattac gcgcggtagg gcagcttggc
    32461 ctgatgtgcc gttggctaat taacgatgtc tcaagccagg tgtccagtgt cagaaatggc
    32521 agtgagacgc tggcgaaagg caccgatgaa ctgaacgaac atacccagca gacagttgat
    32581 aacgttcagc aaacggtggc gaccatgaac caaatggcgg cgtcggtgaa acagaactct
    32641 gccacggcgt cggctgccga taaactttct atcaccgcca gtaatgcggc agtgcagggt
    32701 ggggaggcga tgaccacggt gatcaagaca atggacgata tcgccgacag tacccagcgc
    32761 attggcacca ttacttcgct gattaacgat attgcgtttc agaccaatat tctggccctg
    32821 aatgcggcgg tggaagcggc gcgtgccggc gaacagggca aaggttttgc agtggtggca
    32881 ggggaagtgc gtcatttagc cagccgcagc gctaatgctg ccaacgatat tcgcaagctg
    32941 attgatgcca gtgctgataa ggtgcaatcc ggttcgcagc aggtacacgc cgccggacgg
    33001 acgatggaag atattgtggc acaggtgaaa aacgtcaccc agttgatcgc ccagattagc
    33061 cattcaacgc tggaacaggc cgatgggctt tccagcctga cccgtgcagt ggatgagctt
    33121 aacctgatca cccagaaaaa tgccgagctg gtggaagaga gtgcgcaggt gtcggcgatg
    33181 gtgaaacacc gcgccagccg actggaagac gcggtgacgg tgctgcatta atcggtatgc
    33241 cggatccggt ggttggagca caatgcctga tgcgatgctg gcgcatctta tcaggcctgc
    33301 ggggtgtgta gcgggtgtag gccggataag gcgcgtcaag cgtcgcatcc ggcaattgca
    33361 ccgcgccact ggcggatgcg gcgattaatc cagttgcgta atatcaaaag cggcgcggtc
    33421 gatcaccgca atgatctttt ttatttgtgc atcactgata tcgctctggt tgacgcgtaa
    33481 atccagcacc gctttaaaat tatccagcgc ccgcttcatt tgcgggttct ggcgcagcgc
    33541 cgcgccaacg caacgcgctt tgatgcgttc ttcaatcatc tccacctgtt cgcggttttc
    33601 ttccagccac tgcgcgcctt gttcggtcag cgcaatctgc ttcttaccac cttcctcttc
    33661 gcggatggta atcagcgact gctcctgcag aaaatccagc gttgggtaga tgacgcccgg
    33721 gcttggggtg taattcccct gggttaggtt ctcaatcgct ttaatcaatt cgtaaccgtg
    33781 gctgtcatcg cgcgagagaa tatccagaat caccagacgc aattcaccgt gaccaaagaa
    33841 acgttgccga cgaccgccgc cgcgaccgtg gcgaccaccg cagcattgac cgtgttcatg
    33901 ctggtgaccg tgtccgcagt gctcgtgttc tgacttctca cctttgcagc agccctcatg
    33961 gcgtggctgg ccttcatgtt tacaacaccc ttcgtgatga tggctcatat cagcctcctt
    34021 ttgcttaatt agatatatct aatttatatc tgatataagt attactgcaa gtacaaaaat
    34081 gatttttaaa cgcagg
//
LOCUS       DAFNBJ010000036        33369 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000036 DAFNBJ010000000
VERSION     DAFNBJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.036"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            176..382
                     /locus_tag="L2X49_001650"
     CDS             176..382
                     /locus_tag="L2X49_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBN4796749.1"
                     /translation="MYIPQNFLPRSRQKLLRSAPGQSFPSVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            1060..2961
                     /gene="ltrA"
                     /locus_tag="L2X49_001651"
     CDS             1060..2961
                     /gene="ltrA"
                     /locus_tag="L2X49_001651"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBN4796750.1"
                     /translation="MLPDELEKRLDGIEDASKKGYPVRNLYKIACEPGLWLQAYVNIR
                     ANAGALTQGVSTDTIDGFSIERAEKLAKTLRSREYVAKPVRRVQIPKKDGKTRPLGIP
                     GGDDKLAQEVARIILERVYEPVFSEHSHGFRPKRSCETALRSIRPVWNGVKWIVDVDI
                     KGFFDNIPHKLLLNTLAEKIKDKNFLKLIEQWLKAGYVDNWKYHRSYSGTPQGGIISP
                     LLANIYLDKLDRFVEQNLIPAYTSGTKRRANPDMNRLAHKIHKLRKKVDGMATDSESE
                     KEAIKKEIERLLEEKRSIPSQVMNDPNFRRMYYVRYADDFVIGVIGSKKDAEHISRQV
                     RNFITTSLGLEVNEAKTRIRHISEGVNFLGYEIRQADAKKLLKQKMQGRHALRRSTTG
                     IVQLFVPDNIAAKFCHQKKYGCYENVKAVHRSSLQNLSEAEIVLTFNAEMRGLANYYS
                     LALDMKYKLSKLYFIWQISLFKTLANKRRSSVNKVAKSLRQNNGDLAITVQAKNGSRK
                     IEVFKLKHVNRNRTTIVDTEPRTAHITTRTTEIMRRLGARICEYCAKTGRCEVHHVRK
                     LKDLKKGRKAGYKPSLWQLMMIARRRKTMILCASCHDKLHSGKLPDLRQEKGRILLQP
                     PVSSGDSAK"
     gene            3111..3608
                     /locus_tag="L2X49_001652"
     CDS             3111..3608
                     /locus_tag="L2X49_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBN4796751.1"
                     /translation="MGKDPEVRYIPNGGAVANLQVATSETWRDKQTGEMREQTEWHRV
                     VLFGKLAEVAGEYLRKGVQVYIEGQLRTRSWEDNGITRYVTEILVKTTGTMQMLGRAA
                     GAQTQPEEGQQFSAQPQPEPQSEAGTKKGGAKTKGRGRKAAQPEPQLQPPEGDDYGFS
                     DDIPF"
     gene            3671..3904
                     /locus_tag="L2X49_001653"
     CDS             3671..3904
                     /locus_tag="L2X49_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBN4796752.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            3970..5928
                     /locus_tag="L2X49_001654"
     CDS             3970..5928
                     /locus_tag="L2X49_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBN4796753.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIQVDWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEYLREAEGWGWCAGRMEAVGECREDARAYRNLPQPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQMQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWTRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            5983..6417
                     /gene="psiB"
                     /locus_tag="L2X49_001655"
     CDS             5983..6417
                     /gene="psiB"
                     /locus_tag="L2X49_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBN4796754.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            6414..7133
                     /locus_tag="L2X49_001656"
     CDS             6414..7133
                     /locus_tag="L2X49_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBN4796755.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYACAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            complement(7145..7333)
                     /locus_tag="L2X49_001657"
     CDS             complement(7145..7333)
                     /locus_tag="L2X49_001657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HBN4796756.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            7413..7571
                     /locus_tag="L2X49_001658"
     CDS             7413..7571
                     /locus_tag="L2X49_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBN4796757.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            8493..8780
                     /locus_tag="L2X49_001659"
     CDS             8493..8780
                     /locus_tag="L2X49_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796758.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYVLSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            8898..9719
                     /locus_tag="L2X49_001660"
     CDS             8898..9719
                     /locus_tag="L2X49_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBN4796759.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(10016..10663)
                     /locus_tag="L2X49_001661"
     CDS             complement(10016..10663)
                     /locus_tag="L2X49_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBN4796760.1"
                     /translation="MIQKANQQKLVFAWGVVLLVVRTTNLLSLFVEWVKLFTDKVTRG
                     GKMKKWMLAICLMFINEICQATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINP
                     VTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAV
                     GAYNAGFRKSERQNQRRLAYASEVYRIYTGIKSSKGIRVPATKKSLPEINSVQNN"
     gene            10940..11323
                     /locus_tag="L2X49_001662"
     CDS             10940..11323
                     /locus_tag="L2X49_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HBN4796761.1"
                     /translation="MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLE
                     LGLRVYDAQMERKESAFNQTEFNKLLLECVVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ASMVDDIREKVSVEMDRFFPKNDDE"
     gene            11514..12200
                     /locus_tag="L2X49_001663"
     CDS             11514..12200
                     /locus_tag="L2X49_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBN4796762.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVVLWQINPFPDYPFFALNQSGSNTNTSDKLTIWNDLSPGTLLVF
                     SFYMLGVGHATIARELGITDRASEDRIKPVKRKIKEFFEHFDLFRVSCIYKGEIDSLL
                     NIIREFYSVK"
     gene            12294..12521
                     /gene="traY"
                     /locus_tag="L2X49_001664"
     CDS             12294..12521
                     /gene="traY"
                     /locus_tag="L2X49_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HBN4796763.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVTEESESTFKEL"
     gene            12555..12914
                     /gene="traA"
                     /locus_tag="L2X49_001665"
     CDS             12555..12914
                     /gene="traA"
                     /locus_tag="L2X49_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HBN4796764.1"
                     /translation="MDAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGL"
     gene            12929..13240
                     /gene="traL"
                     /locus_tag="L2X49_001666"
     CDS             12929..13240
                     /gene="traL"
                     /locus_tag="L2X49_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HBN4796765.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            13262..13828
                     /gene="traE"
                     /locus_tag="L2X49_001667"
     CDS             13262..13828
                     /gene="traE"
                     /locus_tag="L2X49_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HBN4796766.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            13839..14543
                     /gene="traK"
                     /locus_tag="L2X49_001668"
     CDS             13839..14543
                     /gene="traK"
                     /locus_tag="L2X49_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HBN4796767.1"
                     /translation="MLGGLFFSCSVMAGVGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            14543..15970
                     /locus_tag="L2X49_001669"
     CDS             14543..15970
                     /locus_tag="L2X49_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HBN4796768.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            15960..16550
                     /locus_tag="L2X49_001670"
     CDS             15960..16550
                     /locus_tag="L2X49_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HBN4796769.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            16504..16857
                     /locus_tag="L2X49_001671"
     CDS             16504..16857
                     /locus_tag="L2X49_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HBN4796770.1"
                     /translation="MTEQTETTEIKMNMRNINVITALSVPGKTVSDDFIHAVLSNCAT
                     RIVLPAPEKFGSESLPDNFNMAAVGVMKKGMSMSLFSSPGHIVVAGQSGGGKSVPVKE
                     LIDRIIQKYKEPFDE"
     gene            16850..17101
                     /gene="trbG"
                     /locus_tag="L2X49_001672"
     CDS             16850..17101
                     /gene="trbG"
                     /locus_tag="L2X49_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HBN4796771.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            17098..17613
                     /gene="traV"
                     /locus_tag="L2X49_001673"
     CDS             17098..17613
                     /gene="traV"
                     /locus_tag="L2X49_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HBN4796772.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGGRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            17748..17969
                     /gene="traR"
                     /locus_tag="L2X49_001674"
     CDS             17748..17969
                     /gene="traR"
                     /locus_tag="L2X49_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HBN4796773.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            17962..18438
                     /locus_tag="L2X49_001675"
     CDS             17962..18438
                     /locus_tag="L2X49_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796774.1"
                     /translation="MHKSENEKIDFILDEHERIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQEQLNRLLSGKKLTGRDEKDIRHLWQEKVDAMQESATRQREQDTIRKFTGGNKSE"
     gene            18518..18732
                     /locus_tag="L2X49_001676"
                     /pseudo
     CDS             18518..18732
                     /locus_tag="L2X49_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18760..19122
                     /locus_tag="L2X49_001677"
     CDS             18760..19122
                     /locus_tag="L2X49_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796775.1"
                     /translation="MKSIYLESVLAFIFVGVMAMLICGLFYNDYLEQQPATPEQLTEI
                     TQDIPCAAEAFKEAIKSDTSDYQPEPLSLGKAKELASACRERNEMAEVKRVRENERNK
                     IREKQIQALNDAHSVKER"
     gene            19248..21878
                     /gene="traC"
                     /locus_tag="L2X49_001678"
     CDS             19248..21878
                     /gene="traC"
                     /locus_tag="L2X49_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HBN4796776.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYR
                     VFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLREKGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            21875..22261
                     /gene="trbI"
                     /locus_tag="L2X49_001679"
     CDS             21875..22261
                     /gene="trbI"
                     /locus_tag="L2X49_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBN4796777.1"
                     /translation="MTTTQKTTDVTARRRSHWWWAVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQVWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            22258..22890
                     /gene="traW"
                     /locus_tag="L2X49_001680"
     CDS             22258..22890
                     /gene="traW"
                     /locus_tag="L2X49_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HBN4796778.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            22887..23879
                     /locus_tag="L2X49_001681"
     CDS             22887..23879
                     /locus_tag="L2X49_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HBN4796779.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            23888..24526
                     /gene="trbC"
                     /locus_tag="L2X49_001682"
     CDS             23888..24526
                     /gene="trbC"
                     /locus_tag="L2X49_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HBN4796780.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            24523..26331
                     /gene="traN"
                     /locus_tag="L2X49_001683"
     CDS             24523..26331
                     /gene="traN"
                     /locus_tag="L2X49_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HBN4796781.1"
                     /translation="MKRILPLILALVAGMALADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFRRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFDRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            26358..26615
                     /gene="trbE"
                     /locus_tag="L2X49_001684"
     CDS             26358..26615
                     /gene="trbE"
                     /locus_tag="L2X49_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HBN4796782.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKTDERNNDERNEDE"
     gene            26608..27351
                     /gene="traF"
                     /locus_tag="L2X49_001685"
     CDS             26608..27351
                     /gene="traF"
                     /locus_tag="L2X49_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HBN4796783.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKITPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            27367..27714
                     /gene="trbA"
                     /locus_tag="L2X49_001686"
     CDS             27367..27714
                     /gene="trbA"
                     /locus_tag="L2X49_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HBN4796784.1"
                     /translation="MSEDYLKIFTGVVLLIFVIISGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMNQGAAMVVPMVIGSLPNVTSFDGFRRIFICIMLSSVWS
                     GVMWFFIRGLMTG"
     gene            27833..28117
                     /gene="traQ"
                     /locus_tag="L2X49_001687"
     CDS             27833..28117
                     /gene="traQ"
                     /locus_tag="L2X49_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HBN4796785.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGGHVSH"
     gene            28104..28649
                     /gene="trbB"
                     /locus_tag="L2X49_001688"
     CDS             28104..28649
                     /gene="trbB"
                     /locus_tag="L2X49_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HBN4796786.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAAQPAQPAA
                     GTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            28579..28941
                     /gene="trbJ"
                     /locus_tag="L2X49_001689"
     CDS             28579..28941
                     /gene="trbJ"
                     /locus_tag="L2X49_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HBN4796787.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGRKYRD
                     IPAEMSYAPGEQIKGGQE"
     gene            28941..30314
                     /locus_tag="L2X49_001690"
     CDS             28941..30314
                     /locus_tag="L2X49_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HBN4796788.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            30311..33136
                     /gene="traG"
                     /locus_tag="L2X49_001691"
     CDS             30311..33136
                     /gene="traG"
                     /locus_tag="L2X49_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HBN4796789.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQVIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEITNLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTSTRLSGGMY
                     ADAHAEWGVKPKILGVGGGVGIRGGFKGSLDATDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFALYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIHGEENIVDRQYSELKNNHKQEEIQQNNRYNEENKR
                     QKLMPTPSEDALKQMVDDKKERLKGPL"
     gene            33133..>33369
                     /locus_tag="L2X49_001692"
     CDS             33133..>33369
                     /locus_tag="L2X49_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraS"
                     /protein_id="HBN4796790.1"
                     /translation="MITHYDIKMEMQKLKEVLSVEGVNIPSLLQVIKPGTYVFLWILL
                     WPTFLRLVSVKSDVRDVGFDICASVMMGFLLFVAI"
BASE COUNT         8551 a         7810 c         9163 g         7845 t
ORIGIN      
        1 ggcatcccgg caaaaggccg ccgggatgta aggggcaacg ccgtttcgca aaccgcgctt
       61 cacgtcgttg cggcgggtcc ctgcccgttg ttctgtccgg tgccattcac ctgtttaagc
      121 ccctgtcgcc gggggcaaag acagccacaa ccgtctccgc cagtccaggg gtgaaatgta
      181 catcccgcaa aactttttgc ccagaagcag gcaaaaactt ttgcggtctg cccccggaca
      241 gtcgtttccg tccgtggcag tgatgcccct tgcggcgaag gttctcaaaa aggtgatggt
      301 gaataccgga gggaacaaaa ggcaggtccc cgcaaaacgt caaaaccgcg gctcccgaaa
      361 ctcaaaagat gatgggggct gaaaacatca acagaaggag gcacatcatg gcagttcgtg
      421 gcattaacaa ggtcattctc gtaggagtgc gccgttgaag cgcggtgttt tatggagtgt
      481 tgcatgaaaa gcaacatggc ttagtggtat agccattgca tactgtggtt agcctcgctt
      541 ggtaacaggc tcggagatcc tgaaaaggag aggtgaacat agcatccaca gaaaagggtg
      601 gtgaatacgg aaatcgttat aagtatcacc actcaatgca agatgtatat ggataaactg
      661 ggtttgttga agtcgagatc tatgtgggaa acaggaaacc aataacgcag aacgattgaa
      721 gcgttattcc cgtaatagca caagaaatcc gccctacgtt gattgtgacc actctaacgg
      781 gccagctatt gatgaaatag ttaaacgatc gaaagacatc ctaagtacat cgcatctcca
      841 caccgtgaaa ctacgggact acctaaactg gatttatacc cagcatggta gcggagtctc
      901 catagtactc aaggtcggcc tgtaatgggc tacggcagtc cgcattatac ggaagcagca
      961 aaggtataaa tcaaccggaa gggagatgaa agcctcacag tgctgcgggg aagggagaca
     1021 gttgttagat acatcaatct ctaagaggag ggttagccaa tgctgcctga tgaacttgag
     1081 aaaagattag atggaattga ggatgcttca aaaaaagggt atccagtacg taatctatac
     1141 aaaattgcgt gtgagcccgg actgtggctg caagcctacg tgaatatacg ggccaatgca
     1201 ggtgcactga ctcaaggtgt atcaacagat accattgatg gcttctctat cgaaagagca
     1261 gagaagctgg caaaaacatt gcggagtaga gaatacgtag cgaaacctgt acgcagagtt
     1321 cagatcccaa aaaaggacgg aaaaaccaga ccgctgggga ttccaggtgg agacgataaa
     1381 ctggcgcaag aagtagcaag aattattctg gaaagggttt atgaaccagt attctctgaa
     1441 cactctcatg gattcagacc aaaacgttcg tgtgaaactg cgctcagatc tattcgtcca
     1501 gtctggaacg gagttaaatg gatcgtggat gttgatatca aaggtttctt tgacaatatc
     1561 ccccacaaac tattgctgaa tacactggca gagaaaatta aggataagaa tttcctgaaa
     1621 ttaatcgaac agtggctgaa ggcaggatat gtggacaatt ggaaatacca tcgctcatat
     1681 tctggcacgc cacaaggtgg gataatttct cccttactgg caaatatata cctcgacaaa
     1741 ctggaccgtt ttgtagaaca gaacttaatt cctgcatata caagcggtac aaaacgaaga
     1801 gcgaatccag atatgaacag gctggcccat aagattcata aactacgaaa gaaagtagac
     1861 ggtatggcaa ctgattctga atcagaaaaa gaagccataa agaaagaaat tgaacgactg
     1921 cttgaagaaa aacgttctat accatctcag gtcatgaacg atccaaactt caggcgtatg
     1981 tactatgttc gatatgctga tgacttcgta attggtgtca tcggctcgaa gaaagatgct
     2041 gaacatatat caagacaggt cagaaacttt attactactt cactgggatt ggaagttaat
     2101 gaagcaaaga ccaggatccg ccatatctca gaaggagtta attttttagg ctatgaaatc
     2161 agacaggcag atgcaaagaa attgctgaag cagaaaatgc aagggcggca cgctctccgg
     2221 agatccacca caggaattgt gcagctcttc gttcctgaca atatagccgc taaattttgt
     2281 caccaaaaga aatatggctg ttatgagaat gtgaaggctg tacatcgttc aagtctacaa
     2341 aatctcagtg aagctgaaat tgttctgact ttcaacgcgg aaatgcgcgg attggcgaac
     2401 tactacagtc tggcgctgga catgaaatat aaattatcaa aattatattt catatggcaa
     2461 ataagccttt ttaagaccct agcgaataaa cgacgtagtt cagtgaataa agtcgccaaa
     2521 tcgctacggc aaaataatgg agatctggct attaccgttc aggccaaaaa tggatcccga
     2581 aagattgagg ttttcaaact caaacatgtc aatcgcaacc gaacaacgat tgtcgatacg
     2641 gaaccgagaa ccgcacatat cacaacccgc acaacagaga tcatgcgtcg attaggggca
     2701 aggatctgcg agtattgtgc gaagactggg cgctgcgaag tacatcatgt cagaaagcta
     2761 aaagacctga agaaaggtcg taaggccgga tataaaccca gcctgtggca actaatgatg
     2821 atcgcacgcc gccggaaaac gatgattttg tgcgcaagtt gccacgacaa gttacatagc
     2881 ggaaaactac ccgatctgag acaggaaaag ggaagaatac tcctgcaacc acctgtctca
     2941 agtggggatt ctgctaaatg agtttatccc caataagcta gcaatggaga gccgtatgca
     3001 ttgaaaggtg cacgtacggt tcggaggggg ggtaacggtg atctaactca agataagcag
     3061 gacatattcg ccatttatct tgaaagacca cacgtaccct accctaccgc ctgggaaaag
     3121 atccggaagt ccgttacatc cccaacgggg gcgcagtggc aaacctgcag gtcgccacgt
     3181 cggaaacctg gcgtgacaaa cagacagggg agatgcggga gcagacagaa tggcatcgcg
     3241 tggtgctgtt cggcaagctc gcggaagtgg ctggcgaata tctgcgcaag ggcgtgcagg
     3301 tctacatcga aggtcagctt cgcacccgta gctgggaaga taacggtatc acccgttacg
     3361 tcaccgaaat tcttgttaag accacgggca ccatgcagat gctgggacgt gccgcaggtg
     3421 ctcagactca gccggaagag gggcaacagt tcagcgctca gcctcagccg gaaccacagt
     3481 cggaggccgg tacgaaaaaa ggtggcgcaa aaacgaaagg ccgtggacgt aaggccgcgc
     3541 agccggagcc tcagctgcaa ccgccggagg gtgacgatta cgggttttca gacgatatcc
     3601 cgttctgatc gggctgactg tgataaccgc cccgccccgt tctgtgcggg gcatcaccgg
     3661 agagatgaga atgagcgaat atttcagaat acttcagggg ctgcctgacg gtccttttac
     3721 ccgcaaacat gccgaagctg ttgccgcaca gtaccggaac gtctttatcg aggatgacca
     3781 cggcgaacag tttcgcctgg ttgtccgtaa taacggtgca atggtctggc gtacctggaa
     3841 ttttgaggac ggtgccgggt actggatgaa ccatgtcatc agggatttcg ggattcttaa
     3901 gtaagagagg tgccggacgg ggacacgtcc ggcagacaat cattattacc gttttgggag
     3961 gtactaacta tgtcagttac agagtctaag gcaaaaacgg agcgtaaatc cagccgaaaa
     4021 cctgcaaaaa cgcaggaaac agtcctgtcg gccctgctgg cgcagacgga ggaagtgagt
     4081 gtgccgctgg cctcgctgat taagtctccg ctgaatgtgc gcacggtgcc gtattctgcg
     4141 gagtccgtca gcgaactggc ggactccatt aaaggtgtcg gcctgttaca gaatctggtt
     4201 gttcatgccc tgccaggtga ccgttacggt gtcgccgcag gtggtcgccg actggcagca
     4261 ctcaacatgc tggcagagcg tgacatcatt caggttgact ggcctgtccg tgtgaaggtc
     4321 attccgcagg agctggcgac tgccgcatcg atgaccgaga acggtcatcg tcgggatatg
     4381 caccctgccg aacagattgc cggattccgt gcaatggcgc aggaaggcaa aacacctgca
     4441 caaatcggtg atttgctggg ttattcgccc cgccacgttc agcgaatgct gaaactggct
     4501 gaccttgcgc ctgtcatcct cgatgcgctg gcagaagacc gcatcaccac agagcactgt
     4561 caggcgctgg cgctggagaa cgacaccgcg cgtcaggtgc aggtgtttga agctgcctgt
     4621 cagtcgggat ggggcggtaa accggaagta cagaccattc gtcgtctggt gaccgaaagt
     4681 gaagtggcgg tggcggggaa cactaaattc cgcttcgtgg gggctgatgc cttctcgcca
     4741 gatgaactgc gcactgattt gttcagcgac gatgagggtg gctatgtgga ctgtgttgcg
     4801 ctcgatgccg ccctgctgga aaaactccag gctgttgcag agtaccttcg ggaagccgag
     4861 ggctggggat ggtgtgccgg acgcatggag gccgttggtg agtgccgtga agatgcccgg
     4921 gcataccgca acctgccgca gccggaagca gtgctgacgg aggcggaaga agaacgcctg
     4981 aacgaactga tggcacgtta cgacgcactg gaaaaccagt gtgaggaatc cgacctgctg
     5041 gaagcagaaa tgaagctgat gcgctgcatg gcgaaggtca gagcgtggac gccggagatg
     5101 cgtgccggaa gcggtgtggt ggtgtcctgg cgttatggca acgtgtgtgt ccagcgtggt
     5161 gtgcagttgc gcagtgagga tgacgtggct gacgacgctg accgcacgga acagatgcag
     5221 gagaaagcgt cagtggagga aatcagtctg ccactgctga cgaaaatgtc ttccgaacgc
     5281 acgctggcag tccaggcggc actcatgcag caaccggaca aatctctggc actgctggca
     5341 tggacgctct gcctgaatgt gtttggcagc ggagcgtaca gtaaaccagc acaaatcagc
     5401 ctggaatgta aacattattc gctgaccagc gatgcgccgt cagggaagga aggtgccgca
     5461 ttcatggcgc tgatggcaga aaaagcccgg cttgcagccc tgctgccgga gggatggaca
     5521 cgggacatga cgacgttcct gtcactcagc caggaggtgc tgttatccct gctcagtttc
     5581 tgtaccgcgt gcagtcttaa cggtgtccag acccgcgagt gtggtcacac gtcacgcagt
     5641 ccgcttgact cgctggaaac cgctatcgga ttccacatgc gcgactggtg gcagccgaca
     5701 aaagcaaact tcttcggaca cctgaaaaag ccgcagatta tcgcagccct gaatgaggca
     5761 ggactgtccg gtgctgcacg ggatgcagag aagatgaaga aaggtgatgc ggctgaacat
     5821 gcagagcacc atatgaaaga caaccgctgg gtgcctggct ggatgtgtgc accacatcca
     5881 cagacggata ccactgaacg caccgataac ctggctgatg ccgcctgata aacaaccaca
     5941 ccgccccgcc ggagacgggg cggcagcaag ggagataccg tgatgaaaac tgaactgacc
     6001 ctgaatgtat tacagaccat gagcgcacag gaatatgaag atatccgggc agccggaagt
     6061 gatgaacgtc gtgagctgac acacgccgtg atgcgggaac tggatgcgcc ggacaactgg
     6121 acgatgaacg gcgagtacgg cagcgagttt ggcggatttt tccccgtcca ggtccgtttc
     6181 acgcctgccc acgaacgttt ccacctggcg ttatgttcac cgggcgatgt ctctcaggtc
     6241 tgggttctgg ttctggtgaa tgccggtggt gagccgtttg cggtggttca ggtacagcgg
     6301 cggtttgcac cggaagccgt cagccattca ctggcactgg cggcgtcact ggacgcgcag
     6361 gggtacagcg tcagtgacat catccatatc ctgatggcgg aaggaggtca ggcatgagcg
     6421 cacgttcaca ggcactggtt cctctcagca cagagcaaca ggccgcatgg cgggcggtgg
     6481 cggagaccga aaaacgccgc catcagggta acacgctggc tgaatatccg tatgcgtgtg
     6541 cgtttttccg ctgtctgaac ggaagccgca ggatatcgct gtctgacctg cgatttttca
     6601 tgccgtccct gaccgcagaa gaactgcacg gcaaccgtct tcagtggctg tatgccatcg
     6661 atgtgctgat tgaaacacag ggcgaggtct gcctccttcc ccttcccggt gatgcagcag
     6721 agcgcttgtt cccgtcggtg cggtttcgtg tcagggaacg cagtcgccat aaatccgcgc
     6781 tggtcatgca gaagtacagc cgccagcagg cgagggaggc agagcagaaa gcccgtgcgt
     6841 accaggcgct ggtcgctcag gctgaaatcg aactggcatt ccattcgccg gaaacagtcg
     6901 gaagctggca tgcacgctgg agtgaccggg tggcagagca tgacctggaa accctgttct
     6961 ggcagtgggg cgagcgtttt ccgtcactgg ccggaatgga acgctggcag tggcaggata
     7021 tgccattctg gcaggtcatc gcagaggctg gtatggcggc aagggtggtc ggtcatgctg
     7081 ttcgtgagat ggagcggtgg atggtgccga acaaactccg ggaggcagcg tgatgcggca
     7141 acaatcacac ggatttcccg tgaacggtct gaatgagcgg attattttca gggaaagtga
     7201 gtgtggtcag cgtgcaggta tatgggctat gatgtgcccg gcgcttgagg ctttctgcct
     7261 catgacgtga aggtggtttg ttgccgtgtt gtgtggcaga aagaagatag ccccgtagta
     7321 agttaatttt cattaaccac cacgaggcat ccctatgtct agtccacatc aggatagcct
     7381 cttaccgcgc tttgcgcaag gagaagaagg ccatgaaact accacgaagt tcccttgtct
     7441 ggtgtgtgtt gatcgtgtgt ctcacactgt tgatattcac ttatctgaca cgaaaatcgc
     7501 tgtgcgagat tcgttacaga gacggacaca gggaggtggc ggctttcatg gcttacgaat
     7561 ccggtaagta gcaacctgga ggcgggcgca ggcccgcctt ttcaggactg atgctggtct
     7621 gactactgaa gcgcctttat aaaggggctg ctggttcgcc ggtagcccct ttctccttgc
     7681 tgatgttgta cgggcatgaa caacctgact tcaggaaggt cgtttccctt caggaacggg
     7741 gatgaacgcg cgcctgcggc gcccgttctt ttcccccgcc ttctctggtt atggcctgtc
     7801 agaaatcaca tcaccttccc tgtgattatt ctctttttct tcctgctctg attctgacta
     7861 ctgcagtgtt gtcctgtctg ccgggcgcac cttcggctcc cggctgcctg gcggcatccg
     7921 gtcgtcaggc ccgactccga caggcagctg ctggcgcacc tgccagtctc cgccgaaccc
     7981 gctggcgcgg tttcgggctg tcgctacggt ccgatgcgtg aaaaccccgg gcttgcagcg
     8041 accgttccgg tcgcgccgcc tctgtcgcgc cgtggcctgc gctccttaca tcccggcaaa
     8101 aggccgccgg gatgtaaggc gcaacgccgt ttcgcaaacc gcgcttcacg tcgttgcggc
     8161 gggtccctgc ccgttgttgt gtccggtgcc attcacctgt ttaagcccct gtcgccgggg
     8221 gcatagacag ccacaaccgt ctccgccagt ccaggggtga aatgcacatc ccgcaaaact
     8281 ttttgcccag aagcaggcaa aaacttttgc ggtctgcccc cggacagtcg tttccggccg
     8341 tggcagtgat gccccatgcg gcgaaggttc tcaaacaggt gatggtgaat accggaggga
     8401 acaaaaggca ggtccccgca aaacgtcaaa accgcggctc ccgaaactca acagatgatg
     8461 ggggctgaaa accagagaga ggagaaagca ggatgagtac ccgaaatatc cacgttaaca
     8521 ccgcgtcgta cacgcttctt gttgccggga agaaaaaaaa caccggggaa gagtgggacg
     8581 tcctggaatt cagcagcctg actgagctga aaaaatatcg caaaagccac ccggaaaaga
     8641 tggccttcag ttatagctac gtgctcagtc agggtgtgga taagcagttc cgccatatca
     8701 acattgcgga agccgatcac ttcaaacagt tcctgcgtca gattaagcgt gccggcctcg
     8761 atatccgggc gatctgctga ccggatgtga tgaatatccc tcctgattgt tgcaatcttg
     8821 ttaatggaga tcggggggat gatattaagc ggaaaaattt tttattaaat ttattctttc
     8881 ataaaggagt agctgttatg cgattagctt cccgttttgg tcggtataat tccatccgcc
     8941 gtgaacgtcc tttaacggat gatgaattaa tgcagttcgt gccttcggta ttttccggtg
     9001 ataaacatga gtcccggagt gaacgttata cgtatattcc aacaatcaat atcatcaata
     9061 agttacgtga tgaaggtttc cagccattct ttgcctgtca gagtcgggtt cgtgatttgg
     9121 gacgtcgcga atacagtaaa catatgttac gtcttcgcag ggaagggcat attaacggac
     9181 aggaagttcc tgaaattatc ctgcttaatt cacatgatgg ttcatccagt tatcagatga
     9241 tccccggaat ttttcgtttt gtctgcacaa atggcctggt gtgcggaaat aattttggcg
     9301 aaatccgcgt tccacataaa ggtgatattg tcgggcaggt tatcgaggga gcgtatgaag
     9361 tgctcggtgt ctttgataag gtcactgata atatggaggc gatgaaagaa attcatctta
     9421 acagtgacga gcaacattta tttggcagag ctgcactgat ggtcaggtat gaagatgaaa
     9481 ataaaacgcc agtgacacct gaacagataa ttactccccg tcgtcgggaa gacaaacaga
     9541 acgatctctg gacaacctgg cagcgggttc aggagaatat gataaaaggt ggattatcgg
     9601 ggcgaagtgc ctccgggaaa aataccagga caagagccat tacaggtatt gatggtgata
     9661 taagaatcaa caaggcgtta tgggtgattg ccgaacagtt cagaaagtgg aagtcatgat
     9721 aatataagta aagccccgaa aaaattttcg gggctttact tatattatca tttttcggca
     9781 ttgctgtgtc gtctgatata cctcaatttc atcgaccggc tttccggttg tctgtaaata
     9841 ttgtgcccgc gaaaaatggt ctggcaggag gctgcgcagt taccgggtat atataagatg
     9901 ggttcccgct tcgcggggct tcggccagtc agtattgtca ctcatatgat atttttgtgt
     9961 gtggccttcc atgccgcttt tgcggcataa ccggtatctg acaatgtctg taagattaat
    10021 tgttctgcac gctgttaatt tccggaagtg atttcttcgt ggccggaacc cggatacctt
    10081 tactgctttt tatcccggta taaatccggt aaacctctga tgcgtaggca agacgtctct
    10141 ggttctggcg ttcggatttc ctgaatcctg cattgtatgc accaacggcc tcccaggtga
    10201 cgccccattt tttaaatgct attgccagat aataagcacc ggtataaatg ttcatgcagg
    10261 gatctgttgt cagatgttct ggcttaattc cgtagcgggc cagttcgtta aaatgctggg
    10321 aatctacctg catcagtccg ctgccatatc ccgttaccgg attaataccg atggcattaa
    10381 cccggtaacg ggattctttc catgatattg ctctcagtaa atcaggatct attttgtaat
    10441 cccggcctgc aagatcaaag caatcagtgg cctggcagat ctcatttata aacatcaggc
    10501 agatggctaa catccacttt ttcatttttc cacctctggt gactttatcc gtaaataatt
    10561 taacccactc cacaaaaagg ctcaacaggt tggtggttct caccaccaaa agcaccacac
    10621 cccacgcaaa aacaagtttt tgctgatttg ctttttgaat cattagctta tgttttaaat
    10681 aatgtatttt aatttatttt acattattaa atagggtcgt tgacgaccct atggcgaccc
    10741 tatggcgacc cttgttaggg tcagtactga caaattttat gaaaaacatt tggatatatt
    10801 agggccgccc ctagcagcgc cgtgcgtttt tttatagtat gaccctaggg gcgctgctag
    10861 gggtgctctt cttttttcag accaaactat gagtaaatga aataaaactc ttatttcaga
    10921 atataaaagg tactgtctta tggccagagt aaatctgtat atcagtaatg aggttcatga
    10981 aaaaattaac atgattgttg aaaagcgtcg tcaggaggga gcaagagata aagatataag
    11041 cctttcagga actgcttcaa tgcttcttga attggggctt cgcgtatatg atgcacagat
    11101 ggagcgtaaa gagtctgcgt ttaaccagac agagtttaat aaacttcttc ttgaatgtgt
    11161 tgtaaaaaca cagtcaacgg tggcaaagat tttaggtatt gagtctctca gtcctcatgt
    11221 ctccggaaac ccgaagtttg aatatgccag tatggttgac gatatcagag agaaagtgtc
    11281 tgttgagatg gaccggtttt ttccaaaaaa tgatgatgaa taaatgaaaa tgaagtttga
    11341 gttggttcaa atataagaat ttttatgatt caaaaagatg acaatacgtc agataattag
    11401 tatattaatt acgtggttaa tgccacgtta aaaattgaaa ctgaaaatcg ccgatgcagg
    11461 gagttcagac ctccctgcat cgactgtcca tagaatcctt aacggaggtt cctatgtgtg
    11521 cgctggatcg tagagaaagg ccacttaaca gtcaatctgt aaataaatac atccttaacg
    11581 ttcagaatat ctacagaaat tctcccgttc cggtttgtgt ccgtaacaaa aaccggaaaa
    11641 tcctttatgc caatggggct tttattgaac tcttttccag agaagataaa cccttatccg
    11701 gagagagtta tatacgtctg caggttgaaa tttttctttc atcacttgaa ctggaatgcc
    11761 aggctcttgg acatggctct gcattttgtc gtcgttttaa ttttcatggc gaaatctatc
    11821 agataaggat ggagaatgtt tctttttata atgacgaatc tgttgtttta tggcaaatta
    11881 atccgtttcc tgattatcca ttttttgcgt taaatcagag tggaagtaat acaaatactt
    11941 ctgacaaatt aacgatatgg aatgatcttt cccccgggac attgcttgtt ttctcttttt
    12001 atatgctggg tgttggtcat gcaacaattg ccagagaatt gggtattaca gacagagcat
    12061 ctgaggatcg aattaaacca gtcaaacgga aaataaaaga attttttgaa cactttgatt
    12121 tattcagagt gtcatgtatc tataaagggg aaatagattc gctattaaat ataattcgtg
    12181 aattttatag cgttaagtaa atgttaaata agacacgcca attttggcgt gttttttttc
    12241 gtcggtttct gtgcaatcat aattttatta gtcgggaaac agaggtgaat tgagtgagga
    12301 ggcgtaacgc gagaggcggt ataagcagaa cggtctcggt gtatctcgat gaagatacaa
    12361 ataaccgact tattaaggcc aaagatcgta gtggaagaag taaaacgata gaagtccaga
    12421 tccgtctcag agatcatctg aaacgatttc ctgatttcta taacgaagaa atattcaggg
    12481 aggtgaccga agagagcgag tcaacattta aagaacttta gtttttatct atcaatatta
    12541 aacagaggaa tcatatggac gccgttttaa gtgttcaggg tgcttctgcg cccgtcaaaa
    12601 agaagtcgtt tttttctaaa ttcactcgtc tgaatatgct tcgcctggct cgcgctgtga
    12661 tcccggctgc tgttctgatg atgttcttcc cgcaactggc aatggcagca cagggccagg
    12721 acctgatggc aagcggtaac accacggtta aggcgacctt cggtaaggac tccagtgttg
    12781 ttaaatgggt cgttctggct gaagttctgg tcggtgctgt catgtacatg atgaccaaaa
    12841 acgtcaagtt cctggccggt tttgccatca tctctgtatt tattgctgtg ggtatggccg
    12901 tcgttggcct ctgacaggaa ataaaacgat gtcgggagac gagaataaac ttaagaaata
    12961 tcgtttcccg gaaacactga ctaaccagag ccgctggttt ggcctgccac tggatgaact
    13021 gatccccgca gcaatctgta ttggctgggg tatcacaaca tcgaaatatc tgttcggtat
    13081 tggtgcagcg gttctggttt atttcgggat taaaaaactg aaaaaagggc ggggcagttc
    13141 ctggttacgt gacctgattt actggtatat gccaacagcc ctgctgcgcg gtatttttca
    13201 taatgttccc gattcgtgtt tccggcagtg gattaaatag aactgatacc aggattgtta
    13261 tatggaacac ggtgcccgtt taagtaccag tcgtgtaatg gccatcgcct ttatatttat
    13321 gtcagtgctt attgttctca gcctctctgt taacgtcatt cagggggtga ataactaccg
    13381 tcttcagaat gagcaacgca ctgccgtgac gccaatggca tttaatgccc cctttgccgt
    13441 gtcacagaac agtgccgacg cctcttattt acagcagatg gcgctgtcat ttattgccct
    13501 ccgtctgaat gtttcatcag aaaccgtcga tgcctcacat caggcgcttc tgcaatatat
    13561 ccgcccgggc gcacagaacc agatgaaagt tattctggct gaagaagcga agcgtattaa
    13621 aaacgataac gtgaactcag cctttttcca gaccagtgtt cgtgtctggc ctcagtatgg
    13681 ccgtgtggaa attcgtggtg tgcttaaaac ctggattggt gattcaaaac ctttcactga
    13741 tattaaacat tacatcctta ttctgaagcg ggaaaacggg gtgacctggc tggataattt
    13801 cggggaaaca gacgatgaga aaaaataata cagcaataat attaggcggc ctgttttttt
    13861 cctgcagcgt gatggccgga gtcggtacgc tggcccccac cgtggtgcca atggtgaacg
    13921 gtggtcaggc cagtattgcc atcagcaata ccagcccgaa tctgtttacc gttcccggtg
    13981 accggattat cgccgtgaac agtctggatg gtgccctgac caataatgag cagaccgcct
    14041 ccggcggtgt ggtggtggcc accgtcaaca aaaagccctt tacgttcatt ctggaaacag
    14101 aacgtggtct gaatctttcc attcaggccg ttccccgtga aggcgcgggg cgtaccattc
    14161 agctggtcag tgacctgcgc ggaaccggag aagaagccgg tgcgtgggaa acgtccacgc
    14221 cttacgaatc cctgcttgtg accatcagcc aggccgtccg tggcggaaaa ttacccgcag
    14281 gctggtatca ggtcccggtg acaaaggaaa ccctgcaggc cccggcgggg ctgtcttcag
    14341 tggcagatgc cgtatggacg gggaatcacc tgaagatggt ccgctttgcc gtggaaaata
    14401 aaacgctgtc tgccctgaat atccgggaaa gtgacttctg gcagccggga acccgtgccg
    14461 tgatgttcag ccagcctgcc agccagttac tggcaggtgc gcgcatggat gtgtatgtca
    14521 tccgtgacgg ggagggcaac tgatggccag tatcaatacc attgtgaaac gcaagcagta
    14581 cctgtggctg gggattgtgg ttgtcggtac agcctccgcg attggtgggg cactgtatct
    14641 gtctgatgtg gacatgtccg gtaacggtga aaccgtggct gaacaggagc ctgtgccgga
    14701 tatgaccggt gtggtggata cgacctttga tgacaaggtg cgtcagcatg ccaccaccga
    14761 gatgcaggtg acggcagcgc agatgcagaa gcagtatgag gaaatccgtc gtgagctgga
    14821 tgttctgaac aaacagcgcg gtgatgacca gcgtcgtatt gaaaagttgg gacaggacaa
    14881 tgccgccctg gcagagcagg taaaagccct gggtgctaat cccgtcacgg cgaccggtga
    14941 gcctgtaccg cagatgcctg cctcaccgcc cggcccggaa ggcgaaccac agccaggaaa
    15001 cacccccgta tccttcccgc cgcagggcag cgttgctgtt ccaccgccga cagcgtttta
    15061 tcccgggaat ggtgtcacgc caccaccaca ggtgacgtac cagtctgtgc cggtgcctaa
    15121 ccggatacag cgtaaggtgt ttacccgtaa tgagggaaaa cagggaccat cactgccgta
    15181 cattccgtca ggaagttttg cgaaagccat gctgattgaa ggggcggatg ccaatgcctc
    15241 tgtcaccggt aatgaatcca cggtgccgat gcagctgcgt atcaccggcc tggtggaaat
    15301 gccgaacagc aagacgtatg acgcaacggg atgttttgtg ggtctggaag cctgggggga
    15361 tgtgtccagt gagcgtgcca ttgtacgcac ccgcaatatc agttgcctga aggacggcaa
    15421 aactattgat atgccgatta aggggcatgt cagcttccgg ggtaaaaacg gtatcaaggg
    15481 cgaagtggtg atgcgtaacg gcaaaatcct cggctgggca tggggcgcgg gatttgttga
    15541 cggtatcggt cagggaatgg agcgcgcctc ccagccggct gtcgggctgg gtgccacagc
    15601 cgcttacggg gctggtgatg tcctgaaaat gggtatcggt ggcggcgcat cgaaagccgc
    15661 acagacgctc agtgactact acatcaaacg tgccgaacag tatcacccgg tgataccgat
    15721 tggtgcgggc aacgaagtga ccgtggtgtt ccaggacggc ttccagctga aaaccgtgga
    15781 agagatggcg ctggaacgca cgcagagcag agcggaagaa gacaatccgg aaagtccggt
    15841 tcctgttccg ccatcggctg aaagtcatct taacggcttt aatactgacc agatgctgaa
    15901 gcagctgggc aacctgaatc cgcagcagtt tatgtccggc agccagggag ggggcaacga
    15961 tggcaaataa tatgtcgtcc cgtcaggcgt gccatgccgc ccggtatgtt gtcgcccggg
    16021 ttctgcgagg actgttctgg tgtctgaagt acaccgtcat tctgccactg gccacaatgg
    16081 ccctgatggc tctgtttgtt ttatggaagg acaacaccac gccggggaaa ttactggtta
    16141 aggagattaa cttcgtaaga caaacagcac ctgccggaca gttcccggtc agtgaatgct
    16201 ggttttcttc ctctgattct tcaggcagat cagaaataca ggatatctgc cattaccgcg
    16261 cggctgatgc tgcagattat gtcagggaga ctgaccggtc actgatgcaa ctggtcaccg
    16321 cattatgggc aacactggca ctgatgtatg tctcgctggc agcgatcacc gggaaatatc
    16381 cggtgcgacc aggaaaaatg aaatgtatcc gggtggtaac cgcagatgaa catctgaagg
    16441 aagtttatac ggaagacgct tccctgccgg gaaaaatcag gaaatgccct gtttatcttc
    16501 ctgatgacag aacaaacaga aacaacggag ataaaaatga acatgcgtaa tattaatgtt
    16561 attacagccc tctctgtgcc gggtaaaacc gtgtcagatg attttattca tgctgttctc
    16621 agtaactgcg ccacaagaat cgtacttcct gcaccggaga aattcggttc tgagtcattg
    16681 ccggacaatt ttaatatggc tgctgtcggt gtgatgaaga agggtatgtc gatgagttta
    16741 ttttcttctc cggggcatat tgtggttgcc ggacaatccg gtggcggaaa atctgttccg
    16801 gtgaaagaac taatcgacag aattattcag aaatataagg agcccttcga tgaataagct
    16861 ggtttctgat ggcagcgtca aaaaaataaa ttatccggtg ctgtatgagt ccggaataac
    16921 acctccgtta tgtgaagtca gtgcaccaga acctgatgcg ggtgggaaac gcattgtggc
    16981 atatgtttat aaatcatcac ggagtacggt ttttgaaaat cctgacattg taaaaacctg
    17041 tacggtcagg gatttgaaga aggattttgt gaactgcgat gagaaaggtg agggacaatg
    17101 aaacagattt ctttctttat tcctctgctg gggaccttat tactttccgg ctgtgccgga
    17161 accagtacgg aatttgagtg taacgccacc acatctgata cctgtatgac gatggagcag
    17221 gccaatgaga aggccaaaaa actggagcag tcctcagaag caaagccggt tgcggcatca
    17281 ctgccgcgcc tggctgaagg gaacttccgg acaatgccgg tgcagaccgt caccgcgacc
    17341 actccgtccg gcggcagacc tgcggtgaca gcacatccgg agcagaaact gctggcacca
    17401 cgcccgctgt ttaccgctgc ccgggaagtg aaaacggttg ttccggtcag ttcagttacg
    17461 ccggtaacac ctcctcgtcc gctaagaacg ggtgagcaga cggcagcatt atggatagcg
    17521 ccttatattg ataaccagga tgtttatcat cagccatcca gcgtgttttt tgttattaaa
    17581 ccgtctgcgt ggggaaaacc acgtattaat taactggccc tgaagttgta tatcagccgg
    17641 caatgccggc gggctttctg tattcaaaat ctgcctgtaa aacgagtgcc ctgtgcgtga
    17701 aaagggatgg gcggtagcgt gtcagaacat gtcgggagga atattccgtg agtgatgaag
    17761 ccgatgaagc atattcagtg acagaacaac tgaccatgac aggaataaac cggatacgcc
    17821 agaaaataaa tgttcatggt attcctgttt atctttgtga agcatgcgga aatcctattc
    17881 cggaagcccg gcggaaaata tttcccggtg tgacgttgtg cgttgaatgt caggcgtatc
    17941 aggaaaggca gaggaaacac tatgcataaa tcagaaaatg aaaaaataga ttttattctg
    18001 gatgagcatg agcggatatt cagaaaacag ggttctttgt gtatggcact gggtaagggc
    18061 tttttagtta tagctattct ttgtggtgtt gccacattct gtgtttctgg cctgtatctg
    18121 aaatccctgt gcctgtctgt tgcctgctgt tgtggggtat tcagtcgtat ttttaattat
    18181 tacagcgtac cggaggaatc acgcatggta ctcagtcgtc aggaattact ccggcttatg
    18241 cgtctgactg aagacgtacc agatgtgaga caggaacagc ttaaccgttt gttgtccggt
    18301 aaaaaactga ctggtcggga tgaaaaggat atccggcatc tctggcagga gaaagtggat
    18361 gcaatgcagg agtctgccac ccgtcagcgt gaacaggata ctattcggaa gtttactgga
    18421 gggaataaat ccgaatgaga atctgcagtg ttttatgacc tcataagtta actgaaggga
    18481 tattcagtga ttaatataca agcggaggta ataaagaatg cataccttta tttttcttgt
    18541 gctgataaaa atattaacgg tggcggaagc tggaatatta cacccatgcc aaatacggac
    18601 gtatgcgtgc aggttcttcg gacaatcgaa tcacacggag gcaggggatt tggaaataca
    18661 ggggaatgta ttgaagttaa aacaaataaa cctttgaaca acgtgatgga tacactgaat
    18721 ggggggcaat gagtgcttac tgcttcaaag gaaaatgaaa tgaagtcgat atatttagaa
    18781 tctgttctgg cttttatatt tgttggcgtt atggctatgt tgatatgtgg tcttttttac
    18841 aatgactacc ttgagcaaca acctgcaaca ccagaacaac tgacagagat tacacaggat
    18901 atcccctgtg cagcagaagc atttaaggaa gctattaagt ctgacacatc tgattatcag
    18961 cctgaacctt taagtcttgg taaggcaaaa gagcttgcat cagcgtgtag agaaagaaac
    19021 gaaatggcag aggtgaaacg ggtaagagaa aatgagcgca ataagattcg cgaaaaacaa
    19081 attcaggcat taaatgatgc tcattcagta aaggaacgtt aatcctggtt tcctgttaaa
    19141 agcctccatc tcttatttgt gctgtggttt caggaatggc actgcgtgat atatccacgg
    19201 ccacagactg atattttcct tttttaaacg cactaaggaa tactcatatg agtaataacc
    19261 cacttgaggc cgtcactcag gcggttaact ccctcgtcac agcactgaaa ctgcctgacg
    19321 aatccgcaaa ggccaatgaa gttctgggcg aaatgagctt cccgcagttc agtcgtctgc
    19381 tgccgtaccg tgattacaac caggaatccg gtctgttcat gaatgacacc acgatgggct
    19441 ttatgctgga agccattccc atcaatgggg cgaatgagtc cattgtggag gccctcgatc
    19501 acatgctgcg caccaaactg ccgcgcgggg tgccgttctg catccatctg atgtccagtc
    19561 agctggttgg tgacaggatt gaatacgggc tgcgtgagtt ctcctggtcg ggtgaacagg
    19621 ctgaacggtt taacgccatt acccgggctt attatatgaa tgcggcagcg acacagtttc
    19681 cgctgccgga ggggatgaat ctgcccctga ccctgcgcca ttaccgggta ttcttctcgt
    19741 actgttctcc ctcgaagaaa aaaagccggg ctgatattct ggaaatggaa aacctggtga
    19801 aaatcatccg ggcgtcgtta cagggggcca gtatcaccac acaggcggtg gatgcacagg
    19861 cctttatcga tattgtcggg gagatgatta accataaccc ggactccctg tatccgaaaa
    19921 gacgtcagct ggacccgtat tctgatctga attatcagtg tgtggaggac agttttgatc
    19981 tgaaagtccg ggcggattac ctgacgctgg gcctgcgtga gaagggcagg aacagcacgg
    20041 cccgcatcct gaatttccat ctggcccgta acccggaaat cgccttcctg tggaacatgg
    20101 ccgacaacta cagcaacctg ctgaacccgg aactgtccat ctcctgtccg ttcatcctga
    20161 cgctgactct ggtggtggaa gaccaggtga aaacccacag cgaagccaac ctgaagtaca
    20221 tggacctgga gaaaaagtcg aagacctcct atgccaaatg gtttccgtcc gtggagaaag
    20281 aggcgaagga gtggggggaa ctgcgtcagc ggctgggctc cggtcagtcc tctgtcgtgt
    20341 cctacttcct caacatcaca gccttctgca aggacaataa tgaaacggca ctggaagtgg
    20401 agcaggacat cctgaacagc ttccgtaaaa acggttttga gctgatttca ccgcgcttta
    20461 accacatgcg caatttcctg acctgtctgc cctttatggc cgggaaaggg ttgtttaaac
    20521 agctgaaaga ggccggagtg gtacagcgcg cagagagctt taatgtggcc aacctgatgc
    20581 cgttagtggc ggataacccc ctgacaccgg caggtctgct ggcacccacc taccgtaacc
    20641 agctggcgtt tatcgatatt ttcttccggg ggatgaataa caccaactat aacatggcgg
    20701 tctgtggcac ctccggggcc ggtaaaaccg ggctgataca gccacttatc cgcagcgtgc
    20761 tggactccgg gggctttgcc gtggtgttcg acatggggga tggctacaag tccctgtgtg
    20821 agaacatggg gggcgtatat ctggacggtg aaaccctgcg ctttaacccg tttgcgaaca
    20881 tcaccgatat tgaccagtca gcggagcgtg tccgcgacca gctgtcggtg atggccagcc
    20941 ccaacggtaa cctggatgaa gtgcatgaag gtctgctgct gcaggcagtc agggcatcat
    21001 ggctggccaa agagaacagg gcacgtattg atgacgtggt ggatttcctg aaaaacgcca
    21061 gtgacagcga gcagtatgcc gagtcaccga ccatccgcag ccgtctggac gaaatgattg
    21121 tgctgcttga ccagtacact gctaacggca cttatggcca gtatttcaac tccgatgagc
    21181 cgtccctgcg ggatgatgcg aaaatggtgg tgctggagct gggcggactg gaagaccgtc
    21241 cgtccctgct ggttgcggtg atgttcagcc ttatcattta cattgagaac cggatgtacc
    21301 gcacgccgcg taacctcaag aaactgaacg tcattgatga aggctggcgt ctgctggact
    21361 tcaaaaatca caaggtcggt gaatttattg agaaaggcta ccgtaccgcc cgccgtcata
    21421 ccggtgccta tatcaccatc acacagaaca tcgtcgactt tgactctgac aaggcctcca
    21481 gtgcggcccg tgcagcatgg ggtaactcct cctacaaaat tatcctcaaa cagagtgcga
    21541 aggagttcgc gaaatacaac cagctgtatc cggaccagtt cctgccactg cagcgtgaca
    21601 tgattggtaa gttcggtgcg gccaaagatc agtggttcag ttccttcctg ctgcaggtgg
    21661 aaaaccattc ctcctggcac cgcctgtttg ttgacccgtt aagccgcgcc atgtacagct
    21721 ctgacggccc ggattttgag tttgtgcagc agaaacgtaa agaggggctg agtattcatg
    21781 aggcggtgtg gcagctggcg tggaagaagt cagggccgga gatggcttcg ctggaagcct
    21841 ggctggaaga acatgagaaa tacaggagtg tggcatgacc acaacgcaaa aaacgactga
    21901 cgttacagcc cgtcgacgca gccactggtg gtgggcggtg ccgggatgcc tggcaatggt
    21961 gttactgaac gcagccatca gttacggcat tgtcagactg aacgcaccgg tgactgccgc
    22021 cttcaacatg aaacagaccg tggatgcgtt ttttgacagc gccagtcaga aacaactgtc
    22081 agaagcacag tcaaaagccc tttcagcgcg ttttaacacg gcactggaag ccagcctgca
    22141 ggtatggcag cagaaacacc acgcggtcat tctggtgtcg cctgccgtgg tacagggggc
    22201 accggatatc acccgggaaa tccagcagga tatcgcccgg cgaatgaggg cggagccatg
    22261 agatgccggg ggctgattgc cctgctgata tgggggcaga gtgtggccgc cgccgatctt
    22321 ggtacctggg gcgatctgtg gccggtaaag gagccggaca tgctgacggt gatcatgcag
    22381 cgcctgacgg cactggagca gtccggtgag atgggccgga aaatggatgc ctttaaggag
    22441 cgggtgatcc gcaacagtct gcggccccct gccgtgcccg gtatcggacg cacggagaaa
    22501 tacggcagcc ggttgtttga cccgtccgtc agactggctg cggatatccg ggataacgaa
    22561 gggcgggtgt ttgcccgtca gggtgaagtg atgaatccgc tgcagtatgt tccttttaac
    22621 cagacgctgt atttcatcaa tggcgatgat ccggcgcagg tggcctggat gaaacgccag
    22681 acgccgccca cactggagag caaaattatc ctcgtgcagg gcagtatccc ggagatgcag
    22741 aagtctctgg acagccgtgt ttactttgac cagaacggtg tgctctgcca gcgcctcggc
    22801 attgatcagg tcccggcacg ggtgagcgcc gttcccggcg atcgcttcct gaaggtggaa
    22861 tttattccgg cagaggaggg cagaaaatga agcgaagcct gtggctgctg atgttattcc
    22921 ttctcgccgg tcatgtccct gcagcgtctg cggattctgc ctgtgagggg cgttttgtaa
    22981 acccgatcac ggatatctgc tggagctgta ttttcccgct ctcgctgggc agtatcaaag
    23041 tcagtcaggg taaggtcccc gacacggcga acccgtcgat gcccattcag atttgtccgg
    23101 caccgccgcc gctgttcagg cgtatcgggc tggccattgg ttactgggag ccgatggcgc
    23161 tgacggacgt cacccgatca ccgggatgca tggttaacct gggcttcagc ctgccggctt
    23221 ttggtaaaac ggcacaggga acggcgaaaa aggatgagaa gcaggtgaac ggggcgttct
    23281 atcacgttca ctggtacaaa tacccgctga cgtactggct taacatcatc acatcgctgg
    23341 gctgtctgga aggtggtgac ctggatatcg cttatctttc tgaaatcgac cccacctgga
    23401 cggacagcag cctgaccacc attctcaatc cggaagctgt catctttgcc aatccgatag
    23461 cacagggagc ctgcgcagca gatgccattg ccagcgcctt taatatgcct ctcgatgttc
    23521 tgttctggtg tgccggttcg cagggcagta tgtacccgtt caatggctgg gtgagtaatg
    23581 agtccagtcc gttgcagtcc tccctgctgg tcagtgaacg catggcgttc aagctgcacc
    23641 gtcagggcat gattatggaa accatcggga aaaataacgc cgtctgtaat gaatatccgt
    23701 ccccaatcct gcccaaagaa cgctggcgtt accagatggt gaatatgtac ccggacagcg
    23761 gacagtgcca cccgttcggg cgcagtgtga cccgctggga aaccgggaaa aatccgccca
    23821 atacaaagaa aaacttcggc tacctgatgt ggcgtaaacg taactgtgtc ttcctgtgag
    23881 gtgaatgatg aagctgagta tgaaatctct ggcagcactg ctgatgatgc tgaacggggc
    23941 ggttatggcg tcagaaaacg tgaacactcc tgaaaaccgc cagttcctga agcagcagga
    24001 aaatttaagc agacaactgc gtgaaaaacc tgaccatcag ctgaaagcct gggcggagaa
    24061 gcaggtgctg gaaaaccccc ttcagcgttc agataaccat ttcctggatg agctggttcg
    24121 taaacagcag gcttcgcagg acgggaaacc ccggcagggt gctctgtatt ttgtgtcgtt
    24181 ttccattccc gaagaggggc tgaaacgaat gctgggcgaa acccggcact acggtatacc
    24241 cgccacactg cggggcatgg tgaacaatga cctgaagacc accgctgaag ccgtgctgtc
    24301 cctggtgaaa gacggcgcga ctgatggtgt tcagatcgac ccgacgctgt tttcacagta
    24361 cggcattcgc agtgtaccgg cactggtggt gttctgcagt cagggatacg acatcatccg
    24421 gggaaacctg cgggtcggcc aggcgctgga gaaagtggcc gccacgggtg actgccggca
    24481 ggtggctcat gatttactgg cagggaaagg agattccggg aaatgaaacg tattttacct
    24541 ctgatactgg ctctggttgc cggcatggca ctggctgaca gcaacagtga ttaccgggcc
    24601 ggctctgatt ttgctcacca gatcaaagga cagggaagca gcagtattca gggctttaag
    24661 ccacaggaga gtatccccgg ctataacgcg aatccggacg agacaaaata ctacggtggc
    24721 gtgactgccg gcggggatgg tggcctgaaa aatgacggca ccacggaatg ggcgaccggt
    24781 gaaaccggaa aaaccatcac agagtcattc atgaacaagc cgaaagacat tctttcaccg
    24841 gatgcaccgt ttatccagac cggcagggat gtggtgaacc gggctgacag cattgtggga
    24901 aacaccgggc agcagtgcag tgcgcaggag attagccgga gtgaatacac gaattacacc
    24961 tgtgagcggg atttacaggt ggagcagtac tgcacacgaa ctgcccgtat ggagctgcag
    25021 gggagtacca cgtgggaaac ccggacgctg gagtatgaaa tgagtcagct acctgcacgt
    25081 gaagtgaatg gtcagtatgt tgtctccatt acctctcccg ttaccggtga aattgtcgat
    25141 gcgcattaca gctggagtcg tacttacctg cagaagagtg tacctatgac aattacagta
    25201 ctgggaaccc cactgagctg gaatgccaaa tactcagcag atgcctcatt tacaccagta
    25261 cagaaaacac tgactgccgg tgtggctttt acctcatctc accccgtccg ggtcgggaat
    25321 acaaaattca gacgtcatac ggcaatgaag ctgcgtctgg ttgtcagggt aaaaaaagcc
    25381 tcgtacaccc cgtatgttgt ctggtctgaa agctgcccgt tcagcaagga gctggggaaa
    25441 ctgacaaaaa cagaatgtac ggaggctggc gggaacagga cgctggtgaa agacggtcag
    25501 tcatacagca tgtaccagag ctgctgggca taccgggaca cgtatgtcac gcagtcagca
    25561 gacaagggaa cctgtcagac ctacaccgac aatcctgcct gtaccctggt gtcacaccag
    25621 tgtgcctttt actccgaaga aggtgcctgt ctgcatgaat atgccacgta ctcctgtgag
    25681 tcaaaaacat ccgggaaagt gatggtctgt gggggagacg tattctgtct cgatggcgaa
    25741 tgcgataagg ctcagagcgg gaagagtaat gattttgccg aagccgtttc tcagctggcg
    25801 gcactggccg ctgcaggtaa ggatgttgcg gcgctgaacg gggtggatgt ccgtgctttc
    25861 accggtcagg cgaaattttg taagaaggct gcagccggat acagcaactg ctgtaaggac
    25921 agcggctggg gacaggatat cgggctggcc aaatgcagca gtgatgaaaa agccctggct
    25981 aaagcaaaat caaacaaact gaccgtcagt gtcggagagt tctgttcgaa gaaagtgctg
    26041 ggtgtctgtc tggagaaaaa acgcagctac tgtcagtttg attcgaagct ggcgcagata
    26101 gtccagcagc agggacgcaa cggtcagctg cgtatcagtt ttggcagtgc gaagcatcct
    26161 gactgtcggg ggattacggt tgatgagctg cagaaaattc agttcgacag actggatttc
    26221 actaacttct acgaagacct gatgaataac cagaaaatcc cggacagtgg cgttctgacg
    26281 cagaaagtga aagagcagat tgctgaccag ctgaagcagg caggacagta aggtatggaa
    26341 aataaccggg ggaaatgatg aaagttatat tcacttctaa tcgtcttgtt gattttctta
    26401 tccggctttc gattacagcg attgtcattt ccccggtaat tatctggtcg tgggatacgg
    26461 taaaagaaac cacggcagac gggatgcttg ccgctgcttt cgttatcctt tactccggtg
    26521 tgctcctgtt tattctgtat ttttgttttt ctgccctgac tgacctgcag aaaaccgatg
    26581 aaagaaataa tgatgaaaga aatgaagatg aataaagcat tactgccact gttactctgc
    26641 tgctttattt ttccggcgtc agggaaagat gcaggctggc agtggtataa cgagaaaata
    26701 actccgaagg aaaaagaaaa taaacctgta cctgcagccc cccgtcagga gccggatatt
    26761 atgcagaaac tggccgcgct gcagacggca acaaagcggg cgctgtacga agccattctg
    26821 tatcccggcg tggataattt tgtgaaatat ttccggctgc agaattactg gactcagcag
    26881 gccgggcttt tcaccatgag tgctaaaaag gccatgctgg cacatcctga actggactat
    26941 aacctgcagt acagccatta caacggcacg gtacggaacc agctggcggc agaccaggcg
    27001 cagcagcgac aggccattgc gaaactggct gaacactacg gcatcatgtt tttttaccgg
    27061 gggcaggacc ccatcgacgg gcagctggcg caggtcatta atggcttccg ggatacgtat
    27121 ggtctgagtg ttattcccgt ttccgtggat ggcgtgatta atccgttgtt gccggattcc
    27181 cggactgacc aggggcaggc gcagcgcctc ggcgtgaaat atttcccggc catgatgctg
    27241 gttgacccga aacagggcag tgttcgtccg ttatcatacg gttttatttc gcaggacgac
    27301 ctggcaaaac aattcctgaa cgtttctgaa gattttaaac ccaattttta atcgcggatt
    27361 tgatttatga gtgaagatta tttgaaaata tttacaggtg ttgttctgtt aatatttgtc
    27421 attatttccg gttatttctt ttctgagcgt aatgacagga aaatgtttct cctgagctca
    27481 ctggttttcc ttgttattaa tatcgcgtgt ttatatgtgc tgactgccag tctctggttt
    27541 ctgtgtggtg caattatgaa tcagggcgca gcaatggttg ttccaatggt tattggttca
    27601 ttaccgaacg ttacgagctt tgacgggttc agaagaatat ttatctgtat tatgttgtca
    27661 tcggtatggt ccggagtgat gtggtttttt ataagggggc ttatgacagg ctaagtcata
    27721 taaaacggta ataaaaacca tacaaaataa tgagattgaa ctgattaata tcacacaaac
    27781 gcgaatatta cagacataaa tactctcatg taaataataa ccggagttta acatgataag
    27841 taaacgcaga ttctctttac cccggcttga tattacggga atgtgggtat tttccctggg
    27901 tgtctggttt catatcgtcg cccgtcttgt ttacagcaaa ccctggatgg ctttttttct
    27961 ggcggagctg attgcggcca tccttgtgct gttcggcgca taccaggtgc tggatgcatg
    28021 gattgcccgt gtcagccggg aagagcgtga ggcactggaa gccaggcaac aggccatgat
    28081 ggaagggcag caggagggcg gacatgtctc tcactaaatt actgccggtt attttgttga
    28141 tgatggccac aggtgtgcag gcctccaccc gggatgaaat tgaacgactc tggaatccgc
    28201 agggtatggc cgcgcagcct gcacaaccgg cagcaggcac gtcagccagg acagaaaagc
    28261 cggctccccg ctggttccgc ctcagcaacg gcaggcaggt taacctggcc gactggaagg
    28321 tggtgctgtt tatgcagggg cattgccctt actgtcacca gtttgacccg gtactgaaac
    28381 agctggcaca gcagtacggt ttttccgttt ttccctacac cctggatggt cagggggata
    28441 ccgcttttcc ggaagcatta ccggtgccac cggacgtgat gcagaccttc ttcccgaata
    28501 tcccggtggc cacaccgacc accttcctgg tcaacgtcaa cacgcttgag gcattaccgc
    28561 ttttacaggg ggcaacggat gccgccggtt ttatggcgcg gatggatacc gttctgcaga
    28621 tgtacggagg aaaaaaaggt gcgaaataag caggtggtgt tactcattgc cggaatatcc
    28681 ggcatcgcga cgggaataat cgtcagcctg aatatcccct ttatccatca ggggcttttt
    28741 tatcccgcca gccctgtaga aattgtcgtt tcgctgtgcc ttaccttttc tgtttctgtt
    28801 gtgttttttg tgggggcaat tgtgggatgg atttcggtgt ctgaaatata ttacagccgc
    28861 aggactggac ggaaatatcg ggatattcct gctgaaatgt cgtatgcgcc gggagaacaa
    28921 attaagggag ggcaagaatg atgccacgca ttaaacctct tctggttctt tgtgctgctt
    28981 tactgacggt cacacccgca gcgtcagcgg atgtgaacag cgacatgaat cagttcttta
    29041 acaagctggg cttcgcctcc aataccacac agccgggcgt ctggcagggg caggccgccg
    29101 gttatgctta cggtggctcc ctgtatgccc gtactcaggt caaaaatgtt cagctgattt
    29161 ccatgacgct gccggatatc aatgccggat gtggcggcat cgacgcctat ctcggctcgt
    29221 tcagttttat taatggcgag cagctgcagc gttttgttaa gcagattatg agcaatgctg
    29281 ccggttactt ttttgacctt gccctgcaga caacggtgcc ggaaatcaaa accgcaaaag
    29341 acttcctgca gaaaatggca agcgacatta acagtatgaa cctcagttcc tgccaggctg
    29401 cacaggggat tatcggcggg ctcttccccc ggacgcaggt gtcccagcag aaagtctgtc
    29461 aggacattgc cggtgagagc aatatttttg ctgactgggc ggcttcccgg cagggatgta
    29521 ccgttggcgg gaaatctgac agtgtcaggg ataaagccag cgacaaggat aaggagcggg
    29581 tgaccaaaaa catcaacatc atgtggaatg cgctttccaa aaacagaatg tttgacggca
    29641 acaaagagct gaaagagttt gtgatgacgc tgaccggctc actggtgttt gggcctaacg
    29701 gcgaaatcac acccttgtca gccagaacca ctgaccgctc aattatccgg gccatgatgg
    29761 aaggcggcac agcaaaaatt tatcactgca acgattctga taaatgcctg aaagtggtgg
    29821 cagacacacc ggtgaccatc agccgggata atgcactgaa gtctcagatt actaaacttc
    29881 tggccagcat tcagaacaag gcggtcagtg acacgcctct ggatgacaag gaaaaaggct
    29941 ttatttccag taccaccatc cccgtcttca aatacctggt tgacccgcag atgctcggtg
    30001 tttccaacag tatgatttac cagctgacgg actatatcgg ttacgacatc ctgctgcagt
    30061 acattcagga gctgatacag caggccaggg cgatggtggc cacaggaaat tatgacgaag
    30121 cagttatcga acatattaac gacaacatga atgatgccac ccggcagatt gcggcgtttc
    30181 agtcacaggt gcaggtacag caggatgcgc tgctggttgt cgatcgtcag atgagctaca
    30241 tgcgtcagca gctttccgcc cgcatgctca gtcgttacca gaacaactat cacttcggag
    30301 ggagcacgct gtgaatgaag tttatgtgat tgccggtggt gagtggttgc ggaataacct
    30361 gaacgccatt gccgccttta tgggaaccag gacgtgggat tccattgaaa aaattgcgct
    30421 cacattgtct gttctcgcgg tggccgtgat gtgggtacag cggcacaacg tgatggattt
    30481 gctgggctgg gtggccgtgt ttgtgcttat cagcctgctg gttaatgtcc gcacatcggt
    30541 gcaggttatt gataacagtg acctggtcaa agttcaccgg gtggataatg tgccggtcgg
    30601 actggcgatg ccactttcac tgacgacccg tatcgggcat gcaatggtgg ccagttacga
    30661 gatgatcttc acgcaaccgg acagtgtcac ctacagcaaa acggggatgc tgtttggcgc
    30721 agaactggta tcaaaaagca ccgacttttt gtcccggaat ccggaaatca ccaatctttt
    30781 ccaggattat gtgcagaact gcgtgatggg ggatatttac ctgaaccaca aatatacgct
    30841 ggaggagctg atggcctccg ctgatcccta cacgctgatt ttttcccgcc ccagcccgct
    30901 gcggggcgtt tatgacagca ataataattt cgtaacctgt aaggacgcgt cggtttcgct
    30961 gaaagacaag ctgaatctgg acacacagag tggcggaaaa acctggcatt attatgccca
    31021 gcagctattt ggcggacgcc ctgatcctaa cctgctgttc agtacgctga ttggcgacag
    31081 ttacagttac ttctatggct caagtaaatc agccagccag atcatccgcc agaatgtcac
    31141 catcaatgcg ctgaaggagg ggatcaccag ttatgccgca cgtaatggtg attcggccag
    31201 cctggtgaat ctggccacca cgtcatcgat ggagaaacaa cgtctggcac atgtatcaat
    31261 cggtcatgtg gcgatgcgga cactgcccat gacgcagacc atcctgacgg ggatcgccat
    31321 cggtattttc ccgctcctgg tactggcggc tgtcttcaac aaactgacgc tgtccgtcct
    31381 gaaaggctat gtgtttgccc tgatgtggtt gcagtcatgg ccaatgttgt acgccattct
    31441 gaacagcgcc atgacatttt atgcaaagca gaatggtgca ccggtggtgc tctcggaaat
    31501 ttcacagata cagctgaaat actccgatct ggcttccact gccgggtatc tttccatgat
    31561 gatcccgcca ttatcctgga tgatggtcag ggggctcggg gcaggatttt ccagtgtgta
    31621 cagccatttc gcttcctctg ctatcagccc gaccgccagt gcagcaggca gtgtggttga
    31681 cggtaattac tcctacggca acatgcagac ggagaacgtt aacggcttca gctggagcac
    31741 caacagcacc acgtcgtttg gtcagatgat gtaccagacc ggcagtggcg caaccgccac
    31801 acagacccgt gacggtaata tggtgatgga tgcaagcgga gcgatgtccc ggttaccggt
    31861 cggcatcaat gcaacgcgtc agattgcggc ggcacaacag gaaatggccc gggaagcatc
    31921 gaacagagca gaaagtgccc tgcatgggtt cagcagcagt attgccagtg cctggaacac
    31981 gctcagtcag tttggttcta accgggggag cagtgattct gtcaccggtg gtgctgacag
    32041 cacgatgagt gcacaggact ccatgatggc cagccgtatg cgcagtgcag tggaaagcta
    32101 tgcgaaggcg cataatatca gtaatgagca ggcgactcag gaactggcat caacaagtac
    32161 caggctatct ggtggaatgt atgctgatgc tcatgctgaa tggggggtta aacctaaaat
    32221 tcttggtgtt ggtggtgggg taggcatcag aggtggattt aaggggagcc tggatgcaac
    32281 ggatgatgac tcacattcag caagtagtgg cagcagggct acccatgatg ctcgccatga
    32341 tatcgatgct aaagccagta aggactttaa agaggccagc gattacttta ccagtcgcaa
    32401 agtcagtgaa tccggcagcc atactgacaa taatgccgat tcccgcgtgg accagttgtc
    32461 tgcagccctg aactcagcga aacagagtta cgaccagtac acgacgaata tgacccgcag
    32521 ccatgaatat gctgaaatgg catcccgtac tgaaagcatg agtgggcaga tgagcgaaga
    32581 tttgtcgcaa cagtttgcac tgtatgtgat gaaacacgca ccgcaggatg ccgaagctat
    32641 tctgacaaac accagttcac cggagattgc agaacgccgt cgggcgatgg catggtcttt
    32701 tgttcaggaa caggtgcagc ctggtgttga taatgcctgg cgtgaatccc gtggggatat
    32761 tggtaaagga atggagagcg taccttcggg tggtggcagc caggatatta ttgctgacca
    32821 tcaggggcat caggccatta ttgatcaaag aacgcaggac agtaatatcc gtaatgatgt
    32881 aaaacatcag gttgataata tggtcacaga atataaaggg aatatcggag atactcagaa
    32941 tagtattcat ggcgaagaaa atattgtaga cagacaatat tcagagttaa aaaataatca
    33001 caaacaggaa gaaattcaac aaaacaacag atataatgaa gaaaataaac gacagaaatt
    33061 aatgccaaca ccgtctgaag atgcattaaa acagatggtg gatgataaga aagaacgatt
    33121 gaaggggcca ttatgatcac tcactatgat ataaaaatgg agatgcaaaa actaaaggag
    33181 gtactctctg tagagggtgt taatatccca tcattattac aagtcataaa gccgggaact
    33241 tatgtgtttt tatggatttt attatggcca acttttctgc gtttagtatc ggttaaaagt
    33301 gatgtcaggg atgttggctt tgatatatgt gctagtgtaa tgatggggtt cctgcttttt
    33361 gtggctatt
//
LOCUS       DAFNBJ010000037        33138 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000037 DAFNBJ010000000
VERSION     DAFNBJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.037"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..618
                     /gene="lpxT"
                     /locus_tag="L2X49_001693"
     CDS             <1..618
                     /gene="lpxT"
                     /locus_tag="L2X49_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBN4796791.1"
                     /translation="PIDADIFYFFNQKLVESKAFLWLVALTNNRAFDGCSLLAMGMLM
                     LSFWLKENAPGRRRIVIMGLVMLLTAVVLNQLGQALIPVKRASPTLTFTDINRVSELL
                     SVPTKDASRDSFPGDHGMMLLIFSAFMWRYFGKVAGLIALIIFVVFAFPRVMIGAHWF
                     TDIIVGSMTVILIGLPWVLLTPLSDRLITFFDKSLPGKNKHFQNK"
     gene            1030..1596
                     /gene="mepS"
                     /locus_tag="L2X49_001694"
     CDS             1030..1596
                     /gene="mepS"
                     /locus_tag="L2X49_001694"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBN4796792.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1777..3333
                     /locus_tag="L2X49_001695"
     CDS             1777..3333
                     /locus_tag="L2X49_001695"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBN4796793.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHRHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            3415..5229
                     /locus_tag="L2X49_001696"
     CDS             3415..5229
                     /locus_tag="L2X49_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN4796794.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            5230..6324
                     /locus_tag="L2X49_001697"
     CDS             5230..6324
                     /locus_tag="L2X49_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBN4796795.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            6324..7349
                     /locus_tag="L2X49_001698"
     CDS             6324..7349
                     /locus_tag="L2X49_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4796796.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            7351..8940
                     /gene="yejF"
                     /locus_tag="L2X49_001699"
     CDS             7351..8940
                     /gene="yejF"
                     /locus_tag="L2X49_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBN4796797.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(8944..9288)
                     /locus_tag="L2X49_001700"
     CDS             complement(8944..9288)
                     /locus_tag="L2X49_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796798.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(9621..10811)
                     /gene="bcr"
                     /locus_tag="L2X49_001701"
     CDS             complement(9621..10811)
                     /gene="bcr"
                     /locus_tag="L2X49_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBN4796799.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(10839..11534)
                     /gene="rsuA"
                     /locus_tag="L2X49_001702"
     CDS             complement(10839..11534)
                     /gene="rsuA"
                     /locus_tag="L2X49_001702"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBN4796800.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            11684..13444
                     /locus_tag="L2X49_001703"
     CDS             11684..13444
                     /locus_tag="L2X49_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBN4796801.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            13569..13853
                     /gene="rplY"
                     /locus_tag="L2X49_001704"
     CDS             13569..13853
                     /gene="rplY"
                     /locus_tag="L2X49_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBN4796802.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(13992..14999)
                     /gene="yejK"
                     /locus_tag="L2X49_001705"
     CDS             complement(13992..14999)
                     /gene="yejK"
                     /locus_tag="L2X49_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBN4796803.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAQAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            15181..15408
                     /locus_tag="L2X49_001706"
     CDS             15181..15408
                     /locus_tag="L2X49_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBN4796804.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            15428..17188
                     /gene="pbgA"
                     /locus_tag="L2X49_001707"
     CDS             15428..17188
                     /gene="pbgA"
                     /locus_tag="L2X49_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBN4796805.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            17263..17339
                     /locus_tag="L2X49_001708"
     tRNA            17263..17339
                     /locus_tag="L2X49_001708"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17297..17299,aa:Pro,seq:ggg)
     gene            complement(17441..19810)
                     /locus_tag="L2X49_001709"
     CDS             complement(17441..19810)
                     /locus_tag="L2X49_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBN4796806.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAI
                     DTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANV
                     WAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSL
                     ENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNT
                     LSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDA
                     AFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAE
                     LDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREE
                     SSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTI
                     HGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGM
                     RADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNQVKVNDDGKFNN
                     DVAGARGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
     gene            20130..20777
                     /gene="narP"
                     /locus_tag="L2X49_001710"
     CDS             20130..20777
                     /gene="narP"
                     /locus_tag="L2X49_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBN4796807.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(20812..21864)
                     /gene="ccmH"
                     /locus_tag="L2X49_001711"
     CDS             complement(20812..21864)
                     /gene="ccmH"
                     /locus_tag="L2X49_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBN4796808.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21861..22418)
                     /gene="dsbE"
                     /locus_tag="L2X49_001712"
     CDS             complement(21861..22418)
                     /gene="dsbE"
                     /locus_tag="L2X49_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBN4796809.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(22415..24358)
                     /gene="ccmF"
                     /locus_tag="L2X49_001713"
     CDS             complement(22415..24358)
                     /gene="ccmF"
                     /locus_tag="L2X49_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBN4796810.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(24355..24834)
                     /gene="ccmE"
                     /locus_tag="L2X49_001714"
     CDS             complement(24355..24834)
                     /gene="ccmE"
                     /locus_tag="L2X49_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBN4796811.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(24831..25040)
                     /gene="ccmD"
                     /locus_tag="L2X49_001715"
     CDS             complement(24831..25040)
                     /gene="ccmD"
                     /locus_tag="L2X49_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBN4796812.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(25037..25774)
                     /gene="ccmC"
                     /locus_tag="L2X49_001716"
     CDS             complement(25037..25774)
                     /gene="ccmC"
                     /locus_tag="L2X49_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBN4796813.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(25816..26478)
                     /gene="ccmB"
                     /locus_tag="L2X49_001717"
     CDS             complement(25816..26478)
                     /gene="ccmB"
                     /locus_tag="L2X49_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBN4796814.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(26475..27092)
                     /gene="ccmA"
                     /locus_tag="L2X49_001718"
     CDS             complement(26475..27092)
                     /gene="ccmA"
                     /locus_tag="L2X49_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBN4796815.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(27111..27713)
                     /gene="napC"
                     /locus_tag="L2X49_001719"
     CDS             complement(27111..27713)
                     /gene="napC"
                     /locus_tag="L2X49_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBN4796816.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRAICPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(27723..28172)
                     /gene="napB"
                     /locus_tag="L2X49_001720"
     CDS             complement(27723..28172)
                     /gene="napB"
                     /locus_tag="L2X49_001720"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBN4796817.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(28169..29032)
                     /gene="napH"
                     /locus_tag="L2X49_001721"
     CDS             complement(28169..29032)
                     /gene="napH"
                     /locus_tag="L2X49_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBN4796818.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(29019..29714)
                     /gene="napG"
                     /locus_tag="L2X49_001722"
     CDS             complement(29019..29714)
                     /gene="napG"
                     /locus_tag="L2X49_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBN4796819.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(29721..32207)
                     /gene="napA"
                     /locus_tag="L2X49_001723"
     CDS             complement(29721..32207)
                     /gene="napA"
                     /locus_tag="L2X49_001723"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBN4796820.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(32204..32467)
                     /gene="napD"
                     /locus_tag="L2X49_001724"
     CDS             complement(32204..32467)
                     /gene="napD"
                     /locus_tag="L2X49_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBN4796821.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(32457..32951)
                     /gene="napF"
                     /locus_tag="L2X49_001725"
     CDS             complement(32457..32951)
                     /gene="napF"
                     /locus_tag="L2X49_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBN4796822.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
BASE COUNT         8028 a         9070 c         8216 g         7824 t
ORIGIN      
        1 ccaattgatg cggatatttt ttatttcttt aatcagaaac tggtcgaaag taaggccttt
       61 ttgtggctgg ttgcattgac caacaatcgc gctttcgacg gttgttctct gctggcgatg
      121 ggaatgttga tgctgagttt ctggctgaaa gaaaacgccc ctggcagacg acgtatcgtg
      181 attatgggtc tggtcatgct attaactgca gtggtattaa accagctggg tcaggcatta
      241 attcctgtaa aacgggccag cccaacattg acttttaccg atattaaccg cgtcagcgaa
      301 ctgctctctg ttcccacgaa agatgcctca cgagatagct ttcccggcga tcacggcatg
      361 atgctgctta ttttttcggc attcatgtgg cgttatttcg gcaaagttgc aggccttatc
      421 gcccttatta tttttgtggt ttttgcattt cccagagtaa tgattggcgc acactggttt
      481 actgacatca ttgtcggttc gatgaccgtg atattgatcg gtttgccctg ggtgttgctg
      541 acgccattaa gtgatcgatt aatcaccttt tttgacaaat cactaccggg aaaaaacaaa
      601 catttccaaa acaaataact cacagtaatt aacatcatca ggcttatttt tatagtgagg
      661 ataatcctga tgatgcgcac cgtgctttca tccatcgcgc gcaaaaatca ttctctaagt
      721 aaatgaatgg attgcatgcg tttcactcaa tcgtacttta attgaccaat cccgcttatt
      781 aactttctgt atcacttttt cttataaaaa atcatgtaaa accgctcgcc aagaccgcac
      841 caatcgggta atctcgaact cgttttgcct cggcggtaga ttatcctcac agcatataat
      901 tttgtgcgtt agtccacaga tttggcctta aggaattgtt tcaacatgcc caggtaatta
      961 gtctcgtgtc gcttggcatt tttttataac gatatttgtc gttaaggact tcaagggaaa
     1021 acaaacaaca tggtcaaatc tcaaccgatt ttgagatata tcttgcgcgg gattcccgcg
     1081 attgcagtag cggttctgct ttctgcatgt agtgcaaata acaccgcaaa gaatatgcat
     1141 cctgagacac gtgcagtggg tagtgaaaca tcatcactgc aagcttctca ggatgaattt
     1201 gaaaacctgg ttcgtaatgt cgacgtaaaa tcgcgaatta tggatcagta tgctgactgg
     1261 aaaggcgtac gttatcgtct gggcggcagc actaaaaaag gtatcgattg ttctggtttc
     1321 gtacagcgta cattccgtga gcaatttggc ttagaacttc cgcgttcgac ttacgaacag
     1381 caggaaatgg gtaaatctgt ttcccgcagt aatttgcgta cgggtgattt agttctgttc
     1441 cgtgccggtt caacgggacg ccatgtcggt atttatatcg gcaacaatca gtttgtccat
     1501 gcttccacca gcagtggtgt tattatttcc agcatgaatg aaccgtactg gaagaagcgt
     1561 tacaacgaag cacgccgggt tctcagccgc agctaataaa ccgtttggat gcaatccctt
     1621 ggctatcctg acgagttaac tgaaagcact gcttaggcag tgcttttttg ttttcattca
     1681 tcagagaaaa tgatgtttcc gcgtcttgat ccaggctata gtccggtcat tgttatcttt
     1741 taaatgttgt cgtaatttca ggaaattaac ggaatcatgt tcatacgcgc tcccaattct
     1801 ggacgtaagc tcctgcttac ctgcattgtt gcaggcgtga tgattgcgat actggtgagt
     1861 tgccttcaat ttttagtggc ctggcatagg cacgaagtca aatacgacac actgattacc
     1921 gacgtacaaa agtatctcga tacctatttt gccgatctga aatccactac tgaccggctc
     1981 cagccgctga ccttagatac ctgccagcaa gctaaccccg aactgaccgc ccgcgcggcg
     2041 tttagcatga atgtccgtac gtttgtgctg gtgaaagata aaaaaacatt ctgttcatct
     2101 gcgaccggtg agatggacat tccactcaat gaattgattc cggcgctcga cattaataaa
     2161 aacgtcgata tggcgatctt acccggcacg ccgatggtgc cgaacaaacc cgcaatcgtc
     2221 atctggtatc gcaacccttt gctgaaaaat agcggcgtct ttgccgctct gaatctcaac
     2281 ctgacgcctt ctctctttta tagttcacgg caggaagatt acgatggcgt cgccctcatt
     2341 attggtaata ctgcgctatc taccttttct tcacgtttga tgaacgttaa cgaattaacc
     2401 gacatgccag tccgtgaaac taaaattgcg ggcattcctc tgaccgttcg gctttatgca
     2461 gatgactgga catggaacga tgtgtggtac gcatttttac tgggcggcat gagtggaact
     2521 gtcgttggcc tgctctgcta ttacctgatg agcgtacgta tgcgccccgg cagagaaatc
     2581 atgaccgcca tcaagcgcga acaattttac gttgcgtatc aaccggtggt ggatacacaa
     2641 gctttgcgag taacgggcct ggaagtactg ctacgctggc ggcatcctgt agcgggagaa
     2701 attcccccgg atgccttcat taactttgcc gaatcgcaaa agatgattgt gccgctgact
     2761 cagcacctgt ttgagttgat tgcccgcgat gccgcagaat tagaaaaagt actgccggta
     2821 ggcgtcaaat ttggcattaa cattgcgccg gaccatctgc atagcgaaag ctttaaagct
     2881 gatatccaga aactgctcac gtccttgccc gcacaccatt tccagattgt gctggaaatt
     2941 accgagcgcg atatgctgaa agagcaagaa gccacacaac tcttcgcctg gctgcactcg
     3001 gtcggcgtag aaattgctat tgatgacttc ggcaccgggc acagcgcgct tatctatctt
     3061 gagcgtttta cgctcgatta tctgaaaatt gaccgtggat ttatcaacgc catcggtacg
     3121 gaaacgatca cttcccccgt acttgacgcg gtgctgacgc tggcgaaacg cctcaatatg
     3181 ctgacggttg ctgaaggggt cgaaacgccg gaacaggcgc gatggctaag cgaacgcggc
     3241 gttaatttca tgcaaggcta ctggattagt cgcccgttac cgctggacga ttttgttcgc
     3301 tggctaaaga aaccgtatac gccgcagtgg taaggtgtgc ttacgtccct tattattcat
     3361 agtgaaagca tgccggattg cggctaatga tgagtaaaag gaaatccgtt gcagatgatt
     3421 gtgcgcatac tgctgctgtt tatcgctctg ttcacctttg gtgtgcaggc gcaggctatc
     3481 aaggaaagct atgcctttgc cgtgctgggc gaaccccggt acgcgtttaa tttcaaccat
     3541 tttgattatg tgaaccccgc cgcgccaaaa ggtgggcaga taacgttgtc agccctcggc
     3601 accttcgata atttcaaccg ctatgcactg cgcggcaacc cgggcgcacg caccgagcag
     3661 ctgtacgaca cgctatttac gacttccgat gacgaaccag gcagttatta cccgctgatt
     3721 gctgaaagcg cacgctatgc tgacgattat tcctgggtgg aggtcgctat taatccgcgc
     3781 gcccgttttc atgatggttc gcccattact gcccgcgatg tagagtttac ttttcaaaaa
     3841 tttatgaccg aaggcgtgcc gcaatttcgt ctggtctaca aaggcaccac cgtcaaagcc
     3901 attgcgccgt taaccgtgcg cattgagtta gctaaacccg gcaaagaaga tatgttgagt
     3961 ctgttttcgc tgccggtatt tccagaaaag tactggaagg atcacaaact tagcgacccg
     4021 ctcgccacgc ctccgcttgc cagtggtccg taccgcatta cgtcctggaa aatggggcaa
     4081 aatattgtct attcccgcgt gaaagattac tgggcagcaa acttaccggt aaaccgcgga
     4141 cgctggaatt tcgacaccat tcgctacgat tattacctcg atgataatgt cgcctttgaa
     4201 gcgtttaaag caggtgcctt tgatttgcgt atggaaaacg acgccaaaaa ctgggccacg
     4261 cgttataccg gtaaaaattt cgataaaaaa tacatcatca aagatgagca aaagaacgaa
     4321 tcagcccagg atacgcgctg gctggcgttt aatatccaac gtccagtatt cagcgatcgc
     4381 cgggtgcggg aagcaatcac tctcgccttt gactttgaat ggatgaacaa ggcgttgttt
     4441 tacaatgcct ggagtcgcac gaacagttat tttcagaata ccgaatacgc ggccagaaat
     4501 taccccgacg ccgcggagct ggtgcttctg gcaccaatga aaaaagatct accgccagaa
     4561 gtcttcacac aaatctacca gccgccggta tccaaaggcg atggctacga tcgtgacaac
     4621 ctgttaaaag ccgacaaact tctcaacgaa gcgggctggg tgctgaaggg tcagcaacgc
     4681 gttaatgcca caacgggtca gccactcagc tttgaattat tgcttcccgc aagcagcaat
     4741 agtcagtggg tattgccgtt ccagcacagc ctgcaacggc tgggtatcaa catggacatt
     4801 cgcaaggtgg ataactcgca aatcactaac cgcatgcgca gtcgcgacta tgacatgatg
     4861 ccgcgcgtat ggcgggcgat gccgtggccc agttccgatt tacagatttc ctggtcatcg
     4921 gaatatatca attccactta taatgccccc ggcgtgcaaa gcccggttat cgactcgctg
     4981 atcaaccaaa ttattgccgc gcagggaaat aaagaaaaat tactgccgtt ggggcgagca
     5041 ctggatcgcg tattaacgtg gaattattac atgctgccaa tgtggtacat ggcggaagac
     5101 cgtctcgcct ggtgggataa attctcccag ccggccgtgc gccccatcta tagcctcggt
     5161 atcgatacct ggtggtatga cgtcaataaa gcggccaaac tgccgtccgc cagcaaacag
     5221 ggagagtaga tgggcgctta cctgattcgc cgtctgttgc tggtgatccc aacattatgg
     5281 gcgattatca ccatcaactt tttcatcgtg caaattgcgc ctggcggtcc ggtcgaccag
     5341 gccatcgccg ccattgagtt tggtaatgcc ggagtattac ccggcgcagg cggtgaaggt
     5401 gttcgtgcca gccatgcgca aacgggtgtc ggcaatatca gcgacagtaa ttaccgtggc
     5461 ggacgcggat tagatccaga agtgatcgct gagatcactc atcgctacgg ttttgataag
     5521 ccgatccacg aacgttactt caaaatgctc tgggactaca tccgctttga ttttggcgat
     5581 agcctgtttc gcagcgcctc ggtgctgacg ctgattaaag acagtctgcc ggtttccatc
     5641 accctcggat tgtggagcac gctgattatt tatctggtgt cgattccgtt aggcattcgc
     5701 aaagctgttt ataatgggag ccgctttgac gtctggagta gcgcatttat catcatcggc
     5761 tacgccattc cggccttttt gtttgccatc ctgctgattg tcttcttcgc gggcggcagc
     5821 tatttcgacc tgttccccct acgcggcctg gtttccgcta actttgattc gctgccgtgg
     5881 tatcagaaaa tcaccgatta tctgtggcat atcacgctgc cggtgctggc gacagtgatt
     5941 ggtggctttg cggcgctgac catgctgaca aaaaactcat tccttgatga agtgcgcaag
     6001 caatacgtgg tgaccgcgcg tgcgaaaggg gtaagtgaaa aaaatattct ctggaaacat
     6061 gtgttccgca acgccatgct gctggtgatt gccggttttc cggcgacgtt tatcagcatg
     6121 ttttttaccg gctcgctgct gattgaggtg atgttttcac tcaatggtct gggcttactg
     6181 ggctacgaag cgaccgtctc gcgcgattat cctgtaatgt ttggtacctt gtatattttc
     6241 accctgattg gcctgctgct gaatattgtc agtgatatca gctatacgct ggttgatccg
     6301 cgtattgatt ttgagggacg ttaatgtcgc gactcagccc cgtcaatcag gcccgatggg
     6361 cgcgttttcg tcataaccgt cgcggctact ggtcgttatg gattttcctc gtcttgtttg
     6421 gtttgagttt gtgttctgaa cttatcgcca acgataaacc gttgctggtg cgttatgacg
     6481 gcagttggta tttcccgtta ttgaaaaact acagcgaaag cgattttggc ggcccgctgg
     6541 caagtcaggc tgattatcag gacccgtggc tgaaacaacg gctggaaaat aacggctggg
     6601 tactgtgggc accgattcgc tttggtgcta ccagtatcaa ctttgctacc gataagccct
     6661 tcccttctcc accctcccgg caaaactggc tgggaacgga tgccaacggc ggcgatgtgc
     6721 tggcacgcat tctctatggc acgcggatct cggttctgtt tggcctgatg ctgactctct
     6781 gttccagcgt gatgggcgtg ctggcggggg cgctacaagg ctattacggc ggtaaagtcg
     6841 atctctgggg acaacgcttt attgaagtat ggtcggggat gccgacgctg tttttgatta
     6901 ttttactttc cagcgtcgta cagcctaact tctggtggct gctggcaatt actgtcttgt
     6961 ttggctggat gagtctggtc ggcgtggtgc gggcggagtt tttacgtact cgtaatttcg
     7021 actacattcg tgcggcacag gcgcttggcg tcagcgatcg cagtatcatc ctgcgtcata
     7081 tgttgcctaa tgccatggtc gcgaccctca cctttttacc gtttatttta tgtagttcga
     7141 taaccaccct gacctcgctc gatttcctcg gcttcggtct accgctcggt tcaccgtcac
     7201 tcggtgaact gctgttacaa gggaaaaata accttcaggc cccgtggctt gggatcaccg
     7261 ccttcttgtc ggtggcgata ttattgtctt tgctgatctt tattggtgaa gccgtccgcg
     7321 acgcatttga tcctaataag gcggtgtagc atgacgcaaa ctctgttagc gattgaaaat
     7381 ttgtcggtgg gttttcgcca tcagcaaacc gtacgtacag tagtcaatga tgtttcacta
     7441 cagattgagg ctggcgaaac gctggcgctg gtgggtgagt caggttcagg caaaagcgtt
     7501 accgcgctgt caattttacg cctgctccct tccccgccgg ttgaatatct ctccggcgat
     7561 attcgttttc atggcgaatc gctgcttcat gccagcgatc aaacgttgcg cggtgtacgc
     7621 ggtaataaga tcgccatgat ttttcaggaa ccgatggtgt cgttaaatcc attgcatacc
     7681 ctggaaaaac agctttatga agtgctttca ctccaccgcg ggatgcgtcg ggaagcggct
     7741 cgtggcgaaa ttcttaactg ccttgatcgc gttggtatcc gccaggcggc aaaacggctg
     7801 acagattatc cgcatcagct ctccggcggc gaacgccagc gggtgatgat tgcgatggcg
     7861 ctgttaacgc gaccggaatt attaattgcc gatgaaccga ccaccgcact ggacgtctct
     7921 gtccaggcgc agattttaca gctgttgcgc gaactgcaag gcgagctgaa tatgggcatg
     7981 ctgtttatta ctcataacct cagcattgtc agaaaactgg cccaccgcgt ggcggtaatg
     8041 caaaacggtc gctgtgtcga gcaaaataac gccgctacgc tatttgcctc acccactcat
     8101 ccttacacac aaaagctact caacagtgaa ccgtcaggcg acccggtgcc gttgccagaa
     8161 cctgcctcaa cgttgctgga tgttgaacag cttcaggttg ccttccccat tcgcaaaggg
     8221 attttgaagc gcattgtgga tcataatgtg gtggtgaaaa acatcagttt tacgctacga
     8281 gcgggtgaaa cactgggttt agtgggcgag tccggttccg ggaaaagtac gacgggactg
     8341 gcgctgctgc gactgattaa ttctcagggc agcatcatct ttgacggtca gccattgcaa
     8401 aatttaaatc gccgccagct gttacctatt cgtcatcgca ttcaggtggt atttcaggat
     8461 ccaaactcct cgctcaaccc acgactcaac gttttgcaga ttattgagga aggcttacgg
     8521 gttcaccagc cgacgctttc tgccgcacaa cgcgaacaac aagtgatagc cgtgatgcat
     8581 gaagtgggat tagatcctga aacacgccac cgttatccgg cggagttctc tggtggtcag
     8641 cgacaacgta ttgcgattgc cagggcatta attcttaagc cctcgctgat catacttgat
     8701 gaaccgacat catcactcga caaaacggta caggcgcaaa tattgacgct attgaaatca
     8761 ttgcaacaaa agcatcaact ggcctatttg tttatcagcc acgatttgca cgttgtccgc
     8821 gcgttatgtc atcaggttat cgtactgcga caaggggaag tagtggaaca aggaccgtgc
     8881 gcgcgcgtgt ttgccgcacc gcagcaggag tatacgcgtc agctactggc gttgagctga
     8941 cgcttaaaaa ggattgtagt ttgaaaaggg ttcggcaatt gccacaccaa aatttttcaa
     9001 tcggcatgtt gcagcaaact cgtcctggcg atttacaaac aggcacggct caccttcaca
     9061 ctccagcacc gaacatggaa cttcgatgtc gcttaacgcc tggcttagtt tgtacatcac
     9121 cgaccatgca ttatcaccat ccgggctaag caatttaagc gccaccaggc tgttgtcacc
     9181 gcacggtcca ttttgcggaa tcggttcaac cttcgaacct gcgaaaccag cagaccagcg
     9241 aaagttctgc ggtagacgat gaacgattga gagttgtaat gaagtcactt attttccccg
     9301 gaagtacatt tacttcacaa tttgtttaca tcaattttaa cacatcatca acaatccgtc
     9361 tttatacaga tcgtaaagat tgatgcctcg cctgcgtggc ctctatggct ctgatttaag
     9421 taatatctgc gggcgtggtc acgtttgcgg ggctatatgt acccgtttct gcgatctaac
     9481 tcaacctttt taactacaat gatgtgactt tttacataaa ttgattttac ataaaataaa
     9541 catatatcgg gggaatgata gatttgtggt tgacatgcat caacaaagga agccttttag
     9601 cttcctcgtt gtgcaataga tcaccgtttt ttcggtcgac tggcgtacag acagaagaga
     9661 atggagctgg ttgcgcagaa tgcaattgac caaatcatcg gccatgcaga gttaaaggtc
     9721 gcaagagaaa gcaatgcgcc aacaattgcc cctatgccaa aacggaaggt tcctgccagc
     9781 gaagatgccg ttcccgccat atggggaaac tcatcaagaa tgaccgccat cgcattggat
     9841 gacaccatcg acacgcagcc cacaaacgcc gcaacgccaa ccaccagcga ccagaatccc
     9901 agccccagca gcgcactgat gaccatccac gccgccataa taaactgtat ccacaacccc
     9961 gagcggaaca tatttaacgc gccaatacgg cggacgaagc ggctgttaaa aatggtcatc
    10021 acgaacagga aaacaatgtt tagtgcaaag taataaccaa agttttccgg cgcgacgtgg
    10081 ttaatttcaa tgtagacaaa cggtccggcg ctcaggaacg agaacattcc ggcaaagctg
    10141 aaaccgctgg ccagcatata actcaggaca cgcttatgac ggaacagcgc cgcaaagtta
    10201 ccaatagtgg tacgaatgtg aaaaggctgg cgacgctccg gtggtaaggt ttctttgatc
    10261 agaaagaaga tcattgccga agccagaatc gccgccacgg cgaggatcca gaagatgtaa
    10321 tgccagctca gccacaccag cacccagccg ccaactatcg gtgccatcag cggtgcaatg
    10381 gttgtcacca gcatgacaaa cgacatcatc cgcgagaact cttctttcgg gtagatatca
    10441 cgcatcaaag cgttgataac cacgctggcc gcagccgccg ccagcccgtg gaagaaacgc
    10501 atcacaatca gctgatcgat ggtttgtgcc aacgcacacg ccaccgcggc ggcggcaaac
    10561 accagcgtac cgccaagaac caccggctta cgcccgaagc tgtctgccat cggcccgtag
    10621 attaactgcc ccaacgcaaa gcccagaata taagtactga gggtcatctg cgtactgccc
    10681 gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg gcagatacat atcaatcgac
    10741 agcggcatca acatggccag caggccaagg ataaaaacaa tagcaaacga cgaatgctgt
    10801 cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt agacgacgct ggcaatttct
    10861 tcttcagtta acggacgata ttcaccgggg gctaaatcag catccagcgt aatgccgcca
    10921 atacgttcac gatgcagctc aaccacatgg ttacccacgg cggcgaacat gcgtttcacc
    10981 tgatgataac gcccttcgct gatggtcaga cgaacctgcg ttggggtaat cacttccagc
    11041 accgccggct tagtgagatc tttttcgtta tgcagctgca cgcctttagc aaattgctct
    11101 gccgtatcgt cagctacggg tgattccagc gtcaccagat aggtcttctc gcaatgatgg
    11161 cgcggagaag taatgcggtg cgaccactga ccatcatctg tcatcagcac cagaccggtg
    11221 gtatcgatat ccagacgccc tgcggcgtgc agtttccagg ctaccggttc atcaagaaaa
    11281 tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa cgtagccctg aggcttattg
    11341 agcatgaagt aacgtggacc gtgttgctgc gccagcgggt tgccatcgta agcgacatca
    11401 tgttcaggaa gcagtttgaa cgctgcatta cggacgattt cgccatcgac ggtgacacga
    11461 ttgccgcgga tttcacgccc ggcaatagca cggctaacgc cgagttgctg tgcgataaat
    11521 ttatcaagtc gcatgtgtgt gattttgcct gtaaaaaacg gaggtcgggt aatgacccga
    11581 aaatctgaac tgttgtctgc ccagtatagc ggtctaataa catccctcaa gggaaaaaga
    11641 tccatggcat actattagca gcataatcta cctacgcgag accatgattt ttacacttcg
    11701 cccatatcag caagaagccg tggatgccac gctcaaccat tttcgtcgtc ataaaacccc
    11761 tgccgttatc gtgctgccta ctggtgcagg taaaagcctg gtgatagcgg aactggcgcg
    11821 gctggcgcgt ggtcgcgtgc tggtgctggc acacgttaaa gaactggtgg cgcaaaacca
    11881 tgcaaaatat caggcactgg gactggaagc cgatattttt gccgccgggc taaagcgcaa
    11941 agagagccac ggtaaagtgg tatttggcag cgtgcagtcg gtcgcccgta atcttgatgc
    12001 ctttcagggt gaattttcgc tgttgattgt cgatgaatgt caccgtattg gtgatgatga
    12061 agagagccag tatcagcaaa tcctcactca cctgacaaaa gtgaatcccc acttacgcct
    12121 gctggggctg actgccacgc cttttcgatt gggcaaaggc tggatctacc agttccatta
    12181 tcacggcatg gtacgcggcg atgagaaagc ccttttccgt gactgcattt atgagctgcc
    12241 gctgcgttat atgattaaac acggctatct gacgccgcca gaacgactgg atatgccagt
    12301 agtacaatac gatttcagcc gcttgcaggc acagagtaac gggctgttca gcgaagctga
    12361 tctcaaccgt gagctgaaaa aacagcaacg cattacgcca catatcatca gccagatcat
    12421 ggagttcgcc gaaaagcgca aaggggtaat gattttcgcc gcgacggtcg aacacgcaaa
    12481 agagattgtg ggattactgc ccgccgaaga tgctgcactg attactggcg acacccccgg
    12541 cgctgagcgc gatgtgttaa ttgaagattt taaagcccag cgttttcgct atctggtcaa
    12601 cgtcgcagta ctgaccaccg gatttgacgc cccgcacgtc gatcttatcg ccattctgcg
    12661 ccctaccgaa tcagtgagtc tttaccaaca aattgtcggg cgcggtctgc gtctcgctcc
    12721 tggcaagact gattgcttaa ttcttgatta tgcgggtaat cctcacgatc tctacgcgcc
    12781 ggaagttggt acaccgaaag gcaaaagtga caacgtcccg gtacaggttt tctgccctgc
    12841 ctgcggtttt gccaacacct tttgggggaa aacgaccgcc gacgggacat tgattgaaca
    12901 ctttggtcgt cgctgtcagg gatggtttga agatgacgac ggtcatcgcg aacagtgtga
    12961 cttccgtttc cgttttaaaa attgcccgca atgtaacgca gaaaatgata ttgccgcccg
    13021 ccgctgccgc gaatgtgaca ccgtactggt tgatccggac gatatgttaa aagcggcgct
    13081 acgactgaaa gacgcgctgg tattacgctg tagcggcatg tctttgcaac atgggcacga
    13141 cgagaaaggc gaatggttga aaatcaccta ttacgatgaa gacggcgcgg atgtgagtga
    13201 gcgtttccgt ctgcaaacgc ccgcccagcg aactgccttc gagcagcttt ttatccgccc
    13261 gcatacgcgc acaccgggca tccctctgcg ctggatcacc gccgccgata tcctcgccca
    13321 gcaagcgtta ctgcgacacc cggattttgt cgtcgcccgc atgaaaggtc agtactggca
    13381 agtgcgtgaa aaagtgttcg attacgaagg tcgttttcgt cgggcgcacg aattacgcgg
    13441 ttaatggtag ttttgattga tgtataaggc gattgagtat agaatctcgc ccgcttttgc
    13501 atacgcaaag cagatcactt acctgttgct gggtcgcctg tagcaggatt aatttaagag
    13561 agaaagaaat gtttactatc aacgcagaag tacgtaaaga gcagggtaag ggtgcgagcc
    13621 gccgcctgcg tgccgctaac aagttcccgg caatcatcta cggtggcaaa gaagcgccgc
    13681 tggctatcga gctggatcac gacaaagtca tgaacatgca agctaaagct gaattctaca
    13741 gcgaagttct gaccatcgtt gttgacggta aagaaatcaa agttaaagct caggacgtac
    13801 agcgtcaccc gtacaaaccg aagctgcagc acatcgactt cgttcgcgct taattgctga
    13861 ataagttgta aaaaaccccg ctccggcggg gttttttgta tctgcagatt atgcctgatt
    13921 acggtattgc tattttttgc aggccagata aggcgttcac gccgcatctg gcattcagaa
    13981 cacaaaaccg attaattgcc gccagacgtc cggcgctgta attggtcgcg caaattcggc
    14041 ggcgtgcctt taatggtcaa ggtatcggtt gctggatccc agaagatgcg ttcacctaac
    14101 agcatcgcat caaagttaat agtcaagcca ccgccgctac cggcaaactt cgtcaactga
    14161 cgcagtgtgc tacggtctgc cgggaagctc tcttccagtt cgtagccttt ttcagccgca
    14221 aactccgtga aactcacttc gctgacgcct gccagctctt tcgacagcga ctccagttca
    14281 atctcttcgc ctgcttgcag ctgctcgtta cagtagctat aaacctgctg gcgcacattc
    14341 tgccgctcgg ctttatccag ttgcgcttgt gcagtgaaat catccaccgc ttgcagcaag
    14401 ccacggtttt gcgcctttgc attcagccct tcgctggcac caaggaaatc cataaagaaa
    14461 tcggcgacct tgcgccctac ccgccctttc aggaaagtta aatagcgggt cgattccggg
    14521 ttggtttccc attcggttaa atcgatgcgc gcgacgatat ccgcatggtt gatatcgaga
    14581 tagtgcgttg ggttgatgtc gagattttcg ttaacgcgca tactgctcag gttgctcagg
    14641 actgccacca gcagatactc caccgccaga taacggtagt ggcaaaacag cacgaatcca
    14701 ccgtcagcaa aaggatattt cgctaattcg tcacgcaggc gaccagttgc cgcgcggcta
    14761 aatgccagaa agtcctcttc gccctgacgc tgtaagcgca gcgtctgcgc cagttcactc
    14821 tcttcgctaa acaagccgta agctttattt ttggcgctat agacccgatg cagttctgcc
    14881 accatctcaa cgacggtttc tgtcggttcc agcaatgaat cgcgcaacac cagctcaagg
    14941 ttctgctcat cacgcttgat aagctggtgc agagcaatct ggttgatatc cagactcatg
    15001 ataaactctc ctttaagacc gggcggtatt caaccaccgc cggttaagcg acgcaataaa
    15061 agatggcggt cattttacag aaggcagcgt aacgagaatg atttaacgca taaaaaagca
    15121 gaaaaaaaac cgttgctacg gtaatatgtt gccctttcat caactaactg atttcgattt
    15181 atgccacaaa tttcccgcta cagtgatgaa caggttgaac aattgctcgc agagctgctc
    15241 aacgtactgg aaaaacataa ggctccgacc gacctttccc tgatggtgtt aggcaacatg
    15301 gtcactaacc ttatcaacac cagcattgcc ccggcccaac gccaggcaat tgccaactct
    15361 tttgcccgcg ccttacagtc ctctatcaac gaagacaaag cgcactaagg gaaacagata
    15421 acaggttatg gtaactcatc gtcagcgcta ccgtgaaaaa gtctcccaga tggtcagttg
    15481 ggggcactgg tttgcactgt tcaatattct gctttcgctc gtcattggca gccgttacct
    15541 gtttatcgcc gactggccga ccacgcttgc tggtcgcatt tattcctacg taagcattat
    15601 cggccatttc agcttcctgg tgttcgccac ctacttgctg atcctcttcc cgctgacctt
    15661 tatcgtcggc tcccagaggc tgatgaggtt tttgtccgtc attctggcaa cggcgggaat
    15721 gacgctatta ctgatcgata gcgaagtctt tactcgtttc catctccatc ttaatcccat
    15781 cgtctggcag ttggttatca acccagacga aaatgagatg gcgcgcgact ggcagctgat
    15841 gttcatcagc gtgccggtta ttttattgct tgaactggtg tttgcgacgt ggagctggca
    15901 aaagctgcgc agcctgacgc gtcgtcgacg cttcgcgcgc ccgctggccg cattcttatt
    15961 tatcgccttt atcgcctcgc atgtggtgta tatctgggcc gatgccaact tctatcgccc
    16021 gatcaccatg cagcgcgcta acctgccgct ttcgtacccg atgacggcgc gacgttttct
    16081 tgagaagcat ggcctgcttg atgcgcagga gtatcaacgc cgtcttattg agcaaggtaa
    16141 tccagacgcc gtttccgttc agtatccgtt aagcgaactg cgctatcgcg atatgggcac
    16201 cgggcagaat gtcttgttga ttactgtcga tggcctgaac tactcacgct tcgagaagca
    16261 gatgcctgcg ctggcaggtt ttgctgagca aaatatttcg ttcacgcgcc atatgagctc
    16321 cggcaacact acagacaacg gcatctttgg cctgttctat ggcatctcgc cgagctatat
    16381 ggacggcatt ctgtcgaccc gtacgcctgc ggcattaatt actgcgctta atcagcaagg
    16441 ctatcagctg gggttattct catcagatgg ctttaccagc ccgctgtatc gccaggcatt
    16501 gttgtcagat ttctcgatgc cgagcgtacg cacccaatcc gacgagcaga ccgccacgca
    16561 gtggatcaac tggctgggac gctacgcaca agaagataac cgctggttct cgtgggtttc
    16621 tttcaatggt actaacattg acgacagcaa tcagcaggca tttgcacgga aatatagccg
    16681 ggcggcaggc aatgtcgatg accagatcaa ccgcgtgctc aatgcactgc gtgattctgg
    16741 caaactggac aatacggtgg tgattatcac tgccggtcgg ggtattccac tgagcgaaga
    16801 ggaagaaacc tttgactggt cccacggtca tctgcaggtg ccattagtga ttcactggcc
    16861 aggcacgccg gcgcagcgta ttaatgctct gactgatcat accgatctga tgacgacgct
    16921 gatgcaacgc ctgctacatg tcagcacacc tgccagcgaa tattcgcaag gtcaggattt
    16981 gttcaaccct caacgccgtc attactgggt taccgcagcg gataacgata cgctggcaat
    17041 taccaccccg aaaaagacgc tggtgctgaa caataacggt aaataccgca cttacaactt
    17101 acgtggtgaa agagtgaaag atgaaaaacc acagttaagt ttgttattgc aagtgctgac
    17161 agacgagaag cgttttatcg ctaactgatt aattataaat cagttagcga aatatcttac
    17221 ttgcaatcgg tgtggaaaac ggtagtatta gcagccacga gtcggcacgt agcgcagcct
    17281 ggtagcgcac cgtcatgggg tgtcgggggt cggaggttca aatcctctcg tgccgaccaa
    17341 aaatcccaaa aaaaaccaac cattacggtt ggttttttta tatctgcaat taattcgata
    17401 aacagaccgt gacacatcac agcctgttta ttttctgtta tcagaacgtc cagaccacac
    17461 ccgcctgagt attccacggc gattctaccc ctgcgccatt gccatagctg actgacaaat
    17521 gaccgcttaa cgtcggggta aacgatgacc ttatcccagc ctgataaacg ccacgggctc
    17581 cagccacatc attattaaat ttgccatcgt cattaacttt cacctggtta gaatcggcgt
    17641 attcctgacg tacggcggct ttcagccagg gttccagcgt cgtaccattt tgcaggtcca
    17701 tgtgatagct taccgccgtt cccgcttcag cgcgtaatat ccgggtattt cccacatcag
    17761 cgcgcatgcc gtttgataac gtgtagtcct gaccatctgt ggtaaagccg gtaaaggcca
    17821 gatagggtct aacactccac aatccgtcaa cccaacggaa cccgctctca acatgagcac
    17881 ccgcgccgtt actattgtaa tcgccaaacg ctgttgcccc attactcatc ttgccatgga
    17941 tggtgttggc aaaacggtca actttcacca ccccatcaac ataggcaccg ttctgatgct
    18001 cccaaccggc ataagccccc agggtatagc tatcgatatt accttttccg ccgcgatcaa
    18061 aaccaatatc agaatgagag taaccaaaga acaagccgcg aattgtactg ctttcttcac
    18121 gggagaaacg gctatcgata ccgagcgtca ggcccgtcaa tgtttgctca aaaccagctc
    18181 ccgcatcagt ggtcacgttg ttgcgggtgt taattgccga actccacatc gccgtatcgt
    18241 aactaacgcc ttttacgcta ccaagacgct cacgcacggt gtccagttct gcatcaaata
    18301 ccagcggttg tgcggccgcc atattcagca catcagtggt tgaaggggta atttgcgcgc
    18361 gattctctgc caaactccag ctatggttgc cattatccag caaggtatat tcatacgtac
    18421 cgatatcaac aacgcctcca gcattgccca acgtaaatgc agcatcaccg ccgcccgttg
    18481 ttaccagtgt aaggctatct cctgctgccg ggctggcacc ggtgtccgtc acgaatattt
    18541 tgaaatcacc tgttgcctga ccggtgacgt tgagctgatc gctctgatga ttagccatat
    18601 cggtacgcat ataaaaattg ccgtttcccg acagggtatt ggtggtcaac gtaatgaaat
    18661 tttccgcact tgccgtcact gatgaacgag tcactggctc atccatcata atcacatttg
    18721 cgtcagacat cgcaaggttg gctaagttga ggagggtgtt ttggttcgtc gtagtgatat
    18781 cggcaatgat ggtccatacg ctattttcga gcgaaacgtc cgctttgctg gtatccacgg
    18841 caccatgcgt tctaaaagaa gcgccctcag aaaccactac cttacccacc aacattgcgg
    18901 gtgcataatt tatttgcggc ttcatgacct caagaatgcc atcattccct ctgactgaaa
    18961 cgttaaccat tgtgccagcc cagacgttag cgcggccatt gttgataacc atattttcag
    19021 agatggcttt tgaggaatac gtgatacttc cattctgatc tgatcgaccg agatcataca
    19081 ccgcatttgc ctgcactctc gtcccagtat cctttcccag cgattgcata gtggcatgct
    19141 catcaaggat agtatcaatg gcctgcgtgt cctccataac aatgagcccg gaaccatcat
    19201 ccagttcata attttttgac acaccatctt ttatactaaa ttgaccttta ctgtttgtac
    19261 cactcacttc cagcgcattc gttgagacaa ttaattttcc gcctgctttc tgatctaccg
    19321 caatcgctaa tcccccctct ttaacctcca gtaatcctcc cttttcgata gcggcaagga
    19381 cggtaatccc atctttttca acgattaagc gcccgccttt aatcgtggga tcattcgctg
    19441 cgccataaac ctgtagcaca ccattattga gggtagtctg gtttgccgcg ccacccattt
    19501 gaacaatttg ataaccgtca tttaatactg agtattctgc ggtgccattc atttcaatgt
    19561 actgataccc gccgttaatt tcagtattac tacttatacc aaattcttta atatgctgtt
    19621 ctccgccagg attgattttc gtattattgg cggtcccata aaccagctgg atatcttttt
    19681 ccaggacaac accatcaact gtctcaccat actcaactgt tgatgccaat gctatgggtg
    19741 aatataacgc tgtagccacc agaacagata ttgcggaacg acaaagagaa acagaaccag
    19801 attgatgcat tgagctttca tcctatgaaa ttaattgcag ttaaaagcat tgggtacaga
    19861 aaatacccat agctccatac ccggagtcag tttttaaaaa ctgtttaaag aaatgcacaa
    19921 gtattgtgat tgatttttta gttgtttttc ttgatgagaa gctgatgcaa aattccgtct
    19981 ttataatgaa aatgatgcca aagcgaacga caaggttgta gttttcacta catgtccata
    20041 cataaaatgg ggtaacattc acgcgcctgg tagcgttacc aacgctacgc tcaaacataa
    20101 tgattctaat aaaacctcag gagactacta tgcctgaagc aacgcctttt caggtgatga
    20161 ttgtggatga tcatccactt atgcgacgcg gtgttcgtca gttactggag cttgatcctg
    20221 gctttgaagt ggtcgccgaa gcgggcgacg gcgcgagcgc gatcgatctg gcgaatagac
    20281 tggatatcga cgtgatcttg ctggatctca atatgaaagg tatgagtggc ctggataccc
    20341 tcaatgcctt gcgcagggat ggcgttaccg cgcaaattat tatcctgacc gtatccgatg
    20401 cctccagcga tgtctttgcg ctgatagacg caggcgcaga cggttatctg ttgaaagaca
    20461 gcgacccgga agtattgctg gaagcgattc gtgccggagc gaaaggcagc aaagtcttta
    20521 gcgaacgcgt caatcagtac ttacgtgaac gtgaaatgtt tggcgcggaa gaagatccct
    20581 tcagcgtgct gacggagcgc gagctggatg ttctgcacga gctggcacag gggctgtcaa
    20641 ataaacagat tgcctcggtg ttgaatattt ccgagcagac agtaaaagta catattcgca
    20701 atctgctgcg taaactcaat gtccgctcac gcgtggcggc caccattctg ttcctgcaac
    20761 aacgcggggc acaataaaaa tagcccgatg gcatcaccat cgggcctctt tttatttact
    20821 ctcctgcggc gacaaatgtt gcatcgcctg cgcgatacta cgttcaatca ccgcacggcg
    20881 agtatcgttg gcaggtaaga gtttcaacat catctcccac gcggcaacgg cttcgccaaa
    20941 tcgctgctgc tcaaaggcgt taaacgcata catgcttaac acacgaatat tgctatggtc
    21001 cgttctcacc aactggcgca gcagttcgcc gccgagacgg ttgtcgttgg gatcagatga
    21061 acgtgtcaac gcttcagcgt atcccagtgc agcatcgctg ttcttcggat cgaggcgata
    21121 cgcagtggcg tatgcatcgg tggcgatact ggcgttaccc agcgccatgc caacgcggcc
    21181 caacataatc cagccttcta tatcacccgg atttttttgc agttgagtac gcatccccag
    21241 cgcaaggcgc gacatctctt cttcgttgag cggatcggct ttcggatcca gcgccctgtc
    21301 cagcaacgcc ggagcctgtg ccgtggcctg ctgccagatt ttcacctgct gataattgcc
    21361 agtctggtag tagctgacgc cagccacaat tagcgccacc acaatacccg gcagataaac
    21421 aacatatccg gcacgcttac cttccggcac gctttgttca ggaaacgctt ccggcaccac
    21481 gcgtacccga cgccgcgaac gggcgtaaat gacccaaccg ccaatgccaa tagccactac
    21541 cggaagcacc cacagcagca cggtcagcgg cgttaacggc ggatcgtaag tgacgaagtt
    21601 gccgtaacgc gccaccatat aatcgacaat ctctttttta cttttacctt cctgcatcag
    21661 ttcatacact ttctggcgca ggtcggtggc aatcatcgag ttggaatcgg caatgctgtt
    21721 gttctggcat ttcgggcagc gcagttcttc agtgagctga cggaactgtt gttcctgtgc
    21781 ttcatcttta aactgcaaca cgtcgatggt cgccagcgct gagccggaga tcatcagcat
    21841 cagcacgccc aataaaaacc tcattgtgcg gcctccttac tgtatttctc ccacagcggc
    21901 ttgatctctt cttcccagac gcgaggattc agatcgcccg catggcgata gcgaatgatg
    21961 ccgttgccgt caataaggaa cgtttctggc gcgccataga caccgagatc cagccctaac
    22021 atgccgtcgc catcaaacag gcttagcgcg taaggattgc ccagctcttt cagccagctg
    22081 attgccttct ggcgatcgtc tttatagttc atgccgacca cgcgaatgcc ctgcgctgaa
    22141 agctgattca gatattgatg ttccgcacgg caggtcggac accaggtcgc ccagacgtta
    22201 agcagtactg gtttgccctg agtcagcaca tccgcctgat aaaactgccc cggattgtcc
    22261 agtgattcga gacgaaactt cggcacaggc ttgccaatga gcgccgattc cagattggtc
    22321 ggatcatccc cttcggcatt acgcgccagc tgccacagca gcgccgcggc aatcgccagg
    22381 aagataatca acggaattaa caatactttg cgcttcatac ggcctccggc gcagtttttt
    22441 gcggactcac gcgcttacga tagcgaggat caaacagaca cagtagtccg cccaacgcca
    22501 tcatcagccc gcccgcccaa atccagcgaa caaatggttt gtagtaaaga cgcacggccc
    22561 acgcgccgtt ttccagctct tcaccgagcg cggcgtacag gtcacgcgtg atgccgccgt
    22621 caattgccgc ttcggtcatc atcgacccgg cagtgttgta ataacgtttt tccgcataca
    22681 gcaccgtttc cggtttaccc tctcgcgtta cgccaatagt cgccacaccg ccgcgccagt
    22741 tcgggccagt cacctctttg acatcacgga aggtgaagcg atattcatga atatcgacgc
    22801 tatcgccgga cttcatgcgc acatcacgct caacgctata attctggcta aaggcaatgc
    22861 caacaattgt cactgccagc cccaggtgag ccgccaccat cccccaataa ctgaaggtgg
    22921 ttttcgtgcc gcgtgaaata cgtagcgcag cttccgcaat tgccagcacc gcaatccagc
    22981 aggccattgc caggccgagc accgtcatcg ccaccacttt gctttcgaac agccacggca
    23041 acagcagaga cagcaccagc gtagagatga aggcgataat caataaattg cggattttac
    23101 gtgggcgatc gcgcccccag cgcaccagag gaccgacacc cagcagtagc gcaaacggca
    23161 ccatcagcca ggtaaacatg gtgttgaaga acggttcgcc aatcgaaata ctgcccagtc
    23221 ccagttgctt gtgcaccagc ggcagcagcg tccccagcaa caccaccagc atcgcggcga
    23281 ccagcaaaac gttgttcgct aacagcagag attcccgcga ccacagcgca ttgtttacgc
    23341 gtgagcgaac tttgtgtcca cgcgcggcaa acagcagcag cgaaccgcca atcaccagca
    23401 ccataaaggc gaggataaac ataccgcgcg ccggatcaga cgcaaacgcg tgtaccgata
    23461 ccagcacgcc ggaacgcacg aggaaagtcc ccaacagaca caacgagaag gcactgattg
    23521 ccagcaataa tgtccacgct ttgaagctgg cgcgttgttc agtgaccgcc agtgagtgca
    23581 tcagcgcagt ccccaccagc cacggcataa acgaggcgtt ttctaccgga tcccagaacc
    23641 accagccacc ccagccgagt tcgtaatagg cccatgcgga accgagcaca atgccgagcg
    23701 tcaggaaaat ccacgccgcc agcgtccacg ggcgagtaaa acgcgcataa gtgctgtcca
    23761 gacgcccgct cagcaaagaa gcaatggcaa aagcaaacgc caccgagaaa cccacgtacc
    23821 ccatatagag cagaggcgga tggaagatca gccccggatc ctgtaacagc gggttaagat
    23881 cgcgcccttc aatcgggaag ttcggcagcg tgcgtgagaa tgggttagag gtaaagagaa
    23941 tgaacagcaa aaagccgacg ctgaccatcc ccattatcgc cagtacgcgg gccacaatat
    24001 ccagcggaat acgctgacta aaaatcgcca ccgcaaaggt ccagccgctc atcagcagca
    24061 cccacagcag cagtgagcct tcatgtgcgc cccaggtcgc cgccacgcga taccacaccg
    24121 gaagctgggt attggagttg ctggcaacat aggtgacggt aaagtcgttg accacgaaag
    24181 cattgaccag caccagaaat gcgccagcca cagacataaa cagcagccag gcaaacaagc
    24241 gggaagacgc catcatgcgc gcatctccgc gcgccacgcc ccatagcgga tacacggaca
    24301 gcagcagcgc aatccctaac gccaggcaca gcagcccgtt tccaatttcc ggcatcatga
    24361 tgctgggtcc ttataaacac tcgccgggcg acggtggtta gcttccatcg ctttctcaac
    24421 ttctggcggc gtgtagtttt cgtcgtgttt cgccagcact tcttttgcga ggatatgatt
    24481 gcctttttcc agctcgccct gcaccacaac gccctgccct tcacggaaca gatccggcaa
    24541 aatgccttcg taagagacat ccactgagcc ttcagcgtcg taaatggtga aggtcacttt
    24601 cagcgaattg ggatcgcgct gcacactacc cggcatcacc atcccgccaa cgcgcagacg
    24661 ctgaccgact tccggcattt gctgagtttc acgcttgccg tagagaattt cccccggcgt
    24721 ataaaagaga tcgatattcg agcgcagcgc atatagcacc agaccgatag tcagcgccag
    24781 ccctgccaac acggcacagg caatccacaa gcggttttta cggcgaatat tcatgcagcc
    24841 tcctgctgtt gcgcagcacg taaacgcgcc tcacgcgccc gctgttgcgc cacgccacgc
    24901 agaattgcgc gatgttgcat caccgagtgc acgaccaaaa ccaccagcgg aataacggtc
    24961 atcaccaccg ccagccagac aaaaaaggcg taaccgccca ttgcgaaaaa ttcattccag
    25021 gaagcaaatg caggggtcat ttacggcctc ttttcagtat cagttcactc acccacggac
    25081 ggcgtttttc catcagcaaa atcaaattac gcatccgcat cagcgtcagc gtggcagaca
    25141 gtagcaggaa gccaaaaatc gaccagcgca gcggcgaacg catcgccgga tcgatacttt
    25201 gctgcatccg cgttgatccc tgatgcaggg tgttccacca ctccacggag taatgaataa
    25261 tcggcagatt caccacgcca atcagcacca gaatacctgc cgcacggccc gccagacggc
    25321 ggtcgtcgaa ggcgtgccac agggcaatca cacccacata caaaaacagc agcaccagtt
    25381 cagaagtcag acgtgcatcc catacccacc aggtgcccca catcggtttt ccccatgcag
    25441 agccggtaac cagggcaata aaggtaaaca cggcaccaat gggggccatc gccgccaccg
    25501 ccaggttggc cattttcatc tgccagacaa ggccaataaa cgccgccacc gccattgatg
    25561 cataaatgcc catcgaccag atcgccgcag gcacatgcag gtagataatg cggtagctat
    25621 ttccctgctg ataatcagcc ggagcaaagc cgaatcccca gatccagccg acggtaagca
    25681 cgaccacact ggcaattgcc agccacggta taaaccagcc acagatttga tacagccgtg
    25741 gtgggatcgc cagttgatgc agtgttttcc acatagtttc gataccagac tcgaacaaaa
    25801 atcagtaatc cagcgttatt gaatgctgat tcgtaacgct gccgccgtcg caaaaggact
    25861 taatgtcgcg gtgcctgcca gcaacgcgcc taaaattgcc agatacccgt caacgggcaa
    25921 atgcatggaa gccgcgtcca tcgcggcggt ggcaaagatg agtaatggga tagtcagcgg
    25981 taacaccagt atgctgagca gcacaccacc gcgcttaagt cccactgtca acgccacacc
    26041 cggtgcaccg agaaagccaa gcgtaggcgt tcccagcagc agcgtcagcg ccatcacttg
    26101 ccagccataa acatccattc ccagtagcat tgctaccagt ggcgaaagga tgagtaacgg
    26161 cagaccggtt accatccagt gcgccatcac cttcgccagc acaacggcgg gcaagggtaa
    26221 cggcaacaac atcaattgtt caagactgcc gtcctgcaaa tcgtcacgga acagtcgttc
    26281 cagcgccagc aaggatgaaa gcagcgcagc aacccagata atgcccggtg caatacgcgc
    26341 cagcagttgc ggctccggac cgatactgag cggaaaaagg gtaattacaa tcaggaagaa
    26401 ccacagcggg ttggcgattt cggcgctatg gcgaaacgct acacgcagct caagacggaa
    26461 aatgcgccag aacatcatgc ggccctcgtt tgcgtcagtg aaatgcggcg aattttactt
    26521 tcagcaacgt tgagcggctg gtgggtagtc agaatcacaa tccccccctg ctccgtatgc
    26581 tgcgccatac gctgggtcag acgatcgacg ccgttaacgt caatcgcggt aaaaggctcg
    26641 tcgaggatcc ataacgtggc acgggtcagc cacagacgcg ctaaagcgac gcggcgttgt
    26701 tgcccggccg agagctgatt tacaggaata tcttcgaatc cggcaagccc ggcctgcgcc
    26761 agggcttcca gacattgtgc ggtatcgcca tcgcgatgat aaaagtgcag attttctaac
    26821 gccgtcagcc gggttttgat ccccggctga tggcctatcc ataacaggtt ttgatggtag
    26881 ctgtcgcgta cctgatgcaa gggctgccct tgccagagaa cctcgcctgc gtcagggcga
    26941 gacaaccccg tcagcaaacg gagaagcgtt gtcttccccg cgccgttgct accggtgatt
    27001 tgtacccact ctcctgcgtt cagcgtaaat gacaagccac taaataaggt tcgttcatcc
    27061 cgctcacaaa gtaactctct ggcttcaagc atacccacgc aataaccctg ttaaaaacct
    27121 ggctcgactt cacgcatatc gggcagcttg tgcgctatcc ctttatggca atcaatacag
    27181 gtttgcccat ctttcaccgc ctggtcatgc atcttcgcgg caaccgattt ctgggcggtt
    27241 gtatccatat actcgaagtt gtgacagtta cggcactcct gcgagttatt gtccttcatg
    27301 cgccgccact cattctgtgc catcgtcaga cgatgagctt caaatttctg cggcgtgtca
    27361 ataacgccaa aaattttacc atacagctct ttacttgctt tgagcttgcg tatcatcttc
    27421 ggcacaaact cgtgcggaac gtgacaatcc ggacagatcg cacggacgcc gctacggttg
    27481 ttgtagtgca cggaatccat gtattcctga tacaccgtgt tgcgcatttc gtggcagcta
    27541 atgcagaact cttcggtatt ggctttttcc atcccggtgt taaagccacc ccagaagacg
    27601 atgccgccaa caaaaccgat caacagcagc gtccccagcg ccagacggct gggggtacgc
    27661 caccatttcc acaggcgctt aatcagacca ggcttacggt cagaatttcc cataataacc
    27721 tcttatttcc cgtaaccttt tgatggggta aaggtattcc ccacgattgg cgcggtatcg
    27781 gcctgcggta cgtgacattg cagacagaaa taacgacgcg gagccacttc cgcgcccact
    27841 ttgccgtcgc tgtccataaa gtgagtagga ctgatacgcg gcgcgccagt ggtgcgatag
    27901 ctttcgacac cgtggcactg caagcagcgg ttggtattgg tcgttacctg ataaccttca
    27961 acgctatgcg ggatcattgg cggctgattc acatagttca gcggcatccg ttcctgctct
    28021 tttggcatcc gaatggcccc ttcctgtgtc ccggatactt ccggcgactg gctaaaatcg
    28081 acgccattag ccgcccaaac cgcgccgctt accaccaagg ccagcatcgc cgtccattga
    28141 cacagcgctt tcttcaggtc atggcttttc atgatttcgc tcccgaactc catcgtgtag
    28201 ttattgtaaa tacatcctca gaacagacat ccacgcagcg accgcacgtc atgcaatcgc
    28261 ggctggtgac ctgcaccggg ctttgttcat ccagcaccgg ggcacgtagc acatgcggtt
    28321 ccgggcaaac atgaaaacaa tccatacagc ggttacattt ctgccgatcg gtggccgcaa
    28381 cggtaatcac acctttgcta cccagcacgc catacagcgc gccaaccgga caaatgtgcc
    28441 cgcaccagcc gtgttcaacg accagtagat caaataaaaa cagcgcgaga ataagcagcg
    28501 cgccgctgcc gaagcccatt accaggctac gccccatcag agaaacgggg ttgatccatt
    28561 cccaaatgag cgtgccagtt aaggctgatc ccaccagaat gaccaccaac agcacgtagc
    28621 gaatgtggcg agggatcgtc gcagactgat tgaggtcaaa gcgcctgcgt aaccagttcg
    28681 ctaagtcggt aatcgggttc agcgggcaga cccagctgca aaataatcgc tttcccgcca
    28741 gggcgtagag cacggtgata atcaccgcgc cggtcaacgc caccgtggca ggcagatgac
    28801 cactggcaag actttgcagc gtcatcagcg gatcggtcag cggaacggtg tcgaataaca
    28861 ggctactgct gtagttgccg tgcaagatcc acacaccaaa ccatggaccg ctcaaaaaca
    28921 tccccagcac gaagaactga caaaggcgac gtaacaccag ccaacggtga ctgcgccacc
    28981 agcctttttt ctccagcgcc tcgcgcccgg cgtcacgttt acgatttgcc attgttcccc
    29041 tccagccagc cgaagcggta atggtgacct aactcccctt tcgccagtga cagcggtaac
    29101 accttgattg ccggttgttc cagcacgcat actttttcgc acttaccgca accggtacag
    29161 gcgtcgctgt gaaccgtcgg cagaaagcgg gcgtgcttac cggtacgcgt gttgcgctcc
    29221 agctccaggg tgatggcctc atcaattttc gggcattcgc gataacaaac atcgcagcgc
    29281 agcccctgaa agttgagaca gttttcctgg tccaccagta ccgccagccc catccgcgcg
    29341 tcgtcgatcg attcaatctc acgatccagc gcaccgcttg ggcacacttt ggcgcacgga
    29401 atgtcctcac acatttcgca aggaatatcc cgtgcgacaa aatatggcgt gcccgccgac
    29461 agaccagagg ccagcgtcgc cagttttaag gtgtcgtaag ggcaagcctg aacacactga
    29521 ccgcaacgca cacaggcact ggcaaaggcg ttctcgttta tggctccggg cgggcgcaac
    29581 cgcacgccag atgcgcgtgc ggtttgctgt tgtaacccca gcgccacacc cacggcagcc
    29641 agcccgcctg ctgtgcgaac aacatcgcgc agaaagcggc ggcgaccatt ttgaggtttc
    29701 gctgaccggg acataatggc ttacaccttc tccagtttga ccgcgcactt cttgaagtcc
    29761 gtctctttcg agagcggatc ggtcgcatcc agcgtcagtt tgttaaccag ctgtgcggcg
    29821 tcgaagaacg gcatgtacac caggccctgt ggcggacggt tacgaccgcg cgtttcaaca
    29881 atcgagatca cttcgccacg gcgagaaacc actttcactt tgtcgccacg gcgcagatcg
    29941 cgcgctttcg catccagcgg gtgaataaac aagaccgctt ccgggaaggc gcggtgcagt
    30001 tccggcacac ggcgagtcat actgccggtg tgccagtgct ccaggacgcg tccggtagag
    30061 agccacaggt cgtactcttc atccggtgct tccgccgccg gttcgaacgg cagcgcgaag
    30121 atcaccgctt tgccatccgg tttaccgtag aacttgtagc cttcgcccgc tttcacgtac
    30181 gggtcgttac cttcgctgta acgccactgc gtttctttac cgttcaccac cggccagcgc
    30241 agaccgcgcg ctttgtggta gtcatcgaac ggtgccagat cgtgaccgtg accgcgacca
    30301 aaccatgcgt actcttcgaa cagccctttt tgcagataga agcccagctc gcgggattca
    30361 tcgttcagct gatcttccgc cagttcggat accgggaatt tgctcacttc gggggtggca
    30421 tacagaactt cgtacagcgt tttgccacgc agttccggtt tcttcgccag cagctcttcc
    30481 ggccatactt cttcagtttt gaagcggcgg gagaactgga ctaactgcca gagatccgat
    30541 ttcgcttcgc ccggtgcctg tacctgctga cgccagaact gagtacggcg ttcggcgtta
    30601 ccgtaagcgc cctctttctc tacccacatt gcggtcggca ggatcaagtc ggcggccagc
    30661 gcactgactg tcggatacgg atcggagacg atgatgaagt tgcgcggatc gcgccagccc
    30721 ggcatacgct cttcattgat gttcggcccg gcctgcatgt tgttggtaca catggtccag
    30781 taaacattca gcttgccgtc tttcagcgca cggtcttgtg ctaccgcatg cagaccgatt
    30841 ttcgccggaa tggtgccgct cgggatattc cacttcttct cgcagatatc acgatgtttc
    30901 tcgttagtca ccaccatgtc cgcaggcaga cggtgagcaa aggtgcccac ttcacgcgca
    30961 gtaccacacg cggaaggctg cccggtcagg gagaacggac cgcaacccgg ctgggaaatt
    31021 ttgccggtca gcaggtgcag gttgtagacc aggttgttgg cccacacgcc acgagtatgc
    31081 tggttgaagc ccatcgtcca gtaggagatg actttcttgt tcggatcggc atacagctgc
    31141 gccagttgtt ctaactggtc tttcggcacg ccggtcattt cggcagtttt ttccagcgta
    31201 tactcggcaa cgaaggcttt gtaatcttca aagctcatcg gttcggaggc gtcagaaccc
    31261 ggattcttcg ctgctttttc cagcggatgg gtcggacgta aaccgtagcc gatgtccgtc
    31321 gcccctttgc gcaggttaac gtgcttgctg aagaagtcct gatttatcgc attgttttga
    31381 atgatatagt tggcgatgta gttcaggatc accaggtcag attgcggcgt aaagatgatg
    31441 ccgttatccg ccagctcgaa gctacgatgc tggtaggtag aaagcaccgc cacggtgacg
    31501 ttctggttag agagacgacg gttagtgatg cgtgaccaga ggatcgggtg catctccgcc
    31561 atgttagcgc cccacagcac aaacgcgtca gcctgctcga tgtcgtcata gcagcccatc
    31621 ggctcatcca taccaaaggt acgcataaag ccaactactg ccgacgccat acagtgacgt
    31681 gcgttcgggt cgatgttgtt cgaacggaag cccgctttga acagcttgga cgcggcataa
    31741 ccttcccaga tagtccactg accagaaccg aacataccga tcgattccgg tcctttttct
    31801 ttcagggcgg ttttgaattt ctcttccatc acatcgaagg cctgatccca ggtgattggg
    31861 gtaaattcgc cttctttgtc atatttaccg tttttcatac gcagcagcgg ctgcgtcaaa
    31921 cggtctttac cgtacatgat cttgggcagg aaatagccct taatgcagtt caggccacgg
    31981 ttaaccggtg cgtccgggtc gccctgacag gccaccacac gcccctgctg cgttccgacc
    32041 agaacgccgc aaccagtacc gcagaaacgg cacggcgctt tatcccattt aatggcttcc
    32101 tgctgaccaa caacggcgcg ggcaacgccc ggcacgctga gaccggcagc cgccgcagcg
    32161 gccgcaacgg cgttagcttt cataaagcta cgacgactga gtttcatggt gtttcctcac
    32221 cttgctcttc ctgctggtga taaaccagcg acaccgccag cacgccctct acgttgcgta
    32281 ctgactcaat ggtttggatc agcgtttcgc tgtcttctgc ttccaccacc acaatcaact
    32341 gaccgctcgg cgcgtcgctg acagcaactt cacagccggg aaaggcgttc agttgggtgc
    32401 tgatgtctga aattcgttcg cttttggcct gcacgaccag gctgcaaact tgccagttag
    32461 tgtgcatgga gatactccgc agttatggct gataccggac agctggctgc acatgctcca
    32521 catccgttgc aaagttggct attaagttgc ggctggtaga ttccggagag cgtcgggcga
    32581 aagataattg ccattggctc acagctatcc tgacagcggc ggcattcaac tgactgatac
    32641 gccaggcacg cgtccccaat ggtgaactgc aaatcccagg ccctggtgtg gcgcggagaa
    32701 aatagcgatt cggggcaagc ctgcgcgcag gcgtaacaga agctgcactc gttatttttg
    32761 aaattaacgc tcggatagcc gcccgcgccg cgttgcagaa tgttgttttc acaagcatta
    32821 atacaggcgt cgcaacgggt gcaatgggtc agaaaatgag attcatcacc ggaccagggc
    32881 ggacggatac cgttactggc tttgcgccag cgaccagtga gtatgccccg acgggatgca
    32941 tcaatcttca cattgacctt ccatcattaa cgcgctctga aaattgagag cgaccaaata
    33001 aaccgcataa ttaataagcc atttttatag ccgctaagat attaaaggat gtgtcaaaga
    33061 tgcatacccc gatcggggta aaacctgtag caggatcaaa agagtggcgg gaagcgtggc
    33121 aaaaacgggc ttttgctc
//
LOCUS       DAFNBJ010000038        32693 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000038 DAFNBJ010000000
VERSION     DAFNBJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.038"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(259..334)
                     /locus_tag="L2X49_001726"
     tRNA            complement(259..334)
                     /locus_tag="L2X49_001726"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Asn,seq:gtt)
     gene            complement(435..2078)
                     /gene="yeeO"
                     /locus_tag="L2X49_001727"
     CDS             complement(435..2078)
                     /gene="yeeO"
                     /locus_tag="L2X49_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HBN4796823.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            2083..2158
                     /locus_tag="L2X49_001728"
     tRNA            2083..2158
                     /locus_tag="L2X49_001728"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2116..2118,aa:Asn,seq:gtt)
     gene            2320..3582
                     /locus_tag="L2X49_001729"
     CDS             2320..3582
                     /locus_tag="L2X49_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN4796824.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     NGKESRIALGAYPAVSLSDARQQREGVRKMLALNINPVQQRAAERGSRTPDKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKRIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQGLIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRLAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMSHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(3776..5080)
                     /gene="ybtS"
                     /locus_tag="L2X49_001730"
     CDS             complement(3776..5080)
                     /gene="ybtS"
                     /locus_tag="L2X49_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HBN4796825.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCLAQLHINADGTQATFIDDAGEQQWAVDSITDCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGGRRLCEWVKEASTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCLPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDMRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(5108..6388)
                     /gene="ybtX"
                     /locus_tag="L2X49_001731"
     CDS             complement(5108..6388)
                     /gene="ybtX"
                     /locus_tag="L2X49_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HBN4796826.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LMIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRAGYGWG
                     NSEQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAMLIMALSFPLWRITEPTRTAPI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTFMMAPGHLLLPILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGVFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(6381..8183)
                     /gene="ybtQ"
                     /locus_tag="L2X49_001732"
     CDS             complement(6381..8183)
                     /gene="ybtQ"
                     /locus_tag="L2X49_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HBN4796827.1"
                     /translation="MKDNNPADNLAWRVNWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIERFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            complement(8170..9882)
                     /gene="ybtP"
                     /locus_tag="L2X49_001733"
     CDS             complement(8170..9882)
                     /gene="ybtP"
                     /locus_tag="L2X49_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HBN4796828.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VSQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            10139..11098
                     /gene="ybtA"
                     /locus_tag="L2X49_001734"
     CDS             10139..11098
                     /gene="ybtA"
                     /locus_tag="L2X49_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HBN4796829.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPATLALWVPDISADIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRVM"
     gene            11289..17396
                     /gene="irp2"
                     /locus_tag="L2X49_001735"
     CDS             11289..17396
                     /gene="irp2"
                     /locus_tag="L2X49_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HBN4796830.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQARDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCITDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPIRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLATHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     AFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQQQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWFDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEQSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPATLADYLAGV
                     TTVEKTKRPRPVRRRQRRI"
     gene            17484..26975
                     /gene="irp1"
                     /locus_tag="L2X49_001736"
     CDS             17484..26975
                     /gene="irp1"
                     /locus_tag="L2X49_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HBN4796831.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDQPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLAPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDDSWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHFLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFNLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGRCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEIG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDTPTEPAKPTPVAGGNPA
                     LERQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLK
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            26972..28072
                     /gene="ybtU"
                     /locus_tag="L2X49_001737"
     CDS             26972..28072
                     /gene="ybtU"
                     /locus_tag="L2X49_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HBN4796832.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLNSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLL
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            28069..28872
                     /gene="ybtT"
                     /locus_tag="L2X49_001738"
     CDS             28069..28872
                     /gene="ybtT"
                     /locus_tag="L2X49_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HBN4796833.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELQENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGNHFYPIQQARSFFTQIVRHFPHAFSAMTALQKQPSTSER"
     gene            28876..30453
                     /gene="ybtE"
                     /locus_tag="L2X49_001739"
     CDS             28876..30453
                     /gene="ybtE"
                     /locus_tag="L2X49_001739"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HBN4796834.1"
                     /translation="MNSSFKSLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFSLLRLGVIPVLAMPSQRPLD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDHYRHSAQ"
     gene            30584..32605
                     /gene="fyuA"
                     /locus_tag="L2X49_001740"
     CDS             30584..32605
                     /gene="fyuA"
                     /locus_tag="L2X49_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HBN4796835.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         7148 a         9877 c         8980 g         6688 t
ORIGIN      
        1 taaagacgcg acagctacat cccgcataag gcatttttct ctttatcttt gtacggtact
       61 tcatggaaca gagtttttga ccttgcgaat cgtgatgtct gttgggggca acttgctcac
      121 tgaagcgtga gactcgatta agcgcacgaa acacagaaat caaaaaaccc ggtcactttt
      181 ttacaaggta accgggtaaa aataattttt attttttaac tgttttgaga ctcatagaga
      241 tgtctcaaaa ctaaaatttg gctcctctga ctggactcga accagtgaca tacggattaa
      301 cagtccgccg ttctaccgac tgaactacag aggaatcgtg agaacgaggc gaatattagc
      361 gatgcccacc cataatgtca aagcctgttt tttaaatttg aaatcgtttg ctgaaataat
      421 ctgcattttg tcgtttattc cgacacagct ggcttttttt cacacttttg cggctcggat
      481 cgagggtatt tccatagcca acgtccagta accattcgcc agtaaaacag cacggcccgc
      541 acagcccagt cggcaaacat tcccatccag acaccaacca caccccagcc aagcatgatg
      601 cccagcacat aaccgacgac aacccggcaa ccccacatgc tcaacatcga aacccacatg
      661 gcgtaacggg catcacgagc acctttaaat ccagcgggta gcacccatga ggcggaccaa
      721 ataggcataa ataaagcatt tagccaaatc agaatcacaa cgacatgttt aacctgtgga
      781 tcctgggtgt aaaacgatgc cataagcccg gcaaagggag ccgttagcca ggcgatggcc
      841 gttaatccaa gagtggacag ccagaacaca tgccgcaact gaatctctgc ttgcgctatc
      901 tgccctaccc ccaaccttcg gcctgtaatg atcgtagaag cagagccgag cgcacttccg
      961 ggtaagttga taagagccgc aattgaaaac gcgataaaat ttccggcaat aacactggtc
     1021 cccatcccgg caacgaacat ttgggttaat aaccgaccac tggtaaataa cactgattcg
     1081 acactcgctg gaataccaat ccccatgact tcccagataa tgctaaaatt cagcggttta
     1141 aaatagctct ttaacgaaat ccttagcgca ggattaaaac caatcgccag cacccacaaa
     1201 attgcaactg cgccaatata acgagaaatg gttaaaccca acccagcccc gacaaatccc
     1261 agtcccggcc aggagaaaag gccgtaaatc aatatgccgc taataataat attaagaata
     1321 ttcaggctac cgttaatcag tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg
     1381 ctaccaatca aagtgatggc agctgctgga taactgagta ccgtcagctc cagataagtc
     1441 aacgccagtg ctttaacttc tgtcgtggca tcacccgcga cgaaatcaat aatttgttcg
     1501 ccaaaatgat gaataagcgt tgccaacagt acggcaaaca acgtcatgat caccaatgac
     1561 tgccgcgtcg ccaccctcgc tcgtcgtcga tcccgcttac cgagactaaa tgccacaacg
     1621 acagtagtac caagatcgat agctgcaaaa aaagccataa tgaccatatt gaagctgtcc
     1681 gccaatccca cgccggccat cgcatctttt cccagccagc tgaccagaaa agtgctcagg
     1741 acacccatca acaggacaca ggcattctcc atgaagatag gaacagcaag cggggttatc
     1801 tcacgccaga acaacacttt gtagctcttg cgtttagcgt gccagcgagt gccgtgaaca
     1861 acctggcgta aagcagagga gatattcaaa gccgacctta attgcagaaa gtgaaaccac
     1921 atttcaaata atgagggaga atcagcaaag ctgcaaagat tttcgccaac aaattgtctg
     1981 caaatgcaac aaactgttga tagaaacggc aaacagttgg ggaatttaaa aatcgggttt
     2041 gacaaaagat ttttcgccgt taagatgtgc ctcaacagcg attcctctgt agttcagtcg
     2101 gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaat
     2161 ttgctgtttt catgcatcct tgcgaatcat tatatgatgt tggttcaaca ggttagcgtg
     2221 aaaactcttc ccggtgcatt ttgattttac cctccgcatc gggaaaaatt ggtggtcaaa
     2281 tctggggtca ggttagttcg ataatggagc gacccccata tgtcccttac cgacgcgaaa
     2341 atccgcactc tcaaaccttc tgataaaccc tttaaagtct ccgattccca cggtctgtat
     2401 ctgctggtca agccgggtgg ctcacgtcac tggtatctca agtaccgtat taacggtaaa
     2461 gaatcgcgca ttgcgctggg tgcctatcct gccgtctccc tctctgatgc gcgacagcag
     2521 cgtgaaggtg tccgtaaaat gctggcgctg aatatcaacc cggtacagca gcgggctgct
     2581 gaacgtggct cacgaacacc ggataaagtt tttaaaaacg tggcgctggc gtggcataaa
     2641 agtaaccgga aatggtcgca gaacaccgct gaccgtctgc ttgccagcct gaacaatcac
     2701 atctttccgg tcattggcaa cctgcctgta tcagaactta aaccccgtca tttcattgac
     2761 ctgctgaaaa ggatcgagga aaaaggtctg ctggaagttg cgtcccgcac gcggcagcac
     2821 ctgagtaaca taatgcgcca tgcggttcat caggggttaa tcgatacgaa cccggcagca
     2881 aatcttggcg gcgtgaccac acctcctgtc agacggcact atcctgccct gccactggag
     2941 cggctgcctg aactgcttga acgtattggg gcatatcatc agggtcgtga actgacgcgg
     3001 ttggccgtcc tgctgatgct gcatgtgttc attcgctcca gtgaactgcg tttcgcccgc
     3061 tggtcagaga ttgatttcac aaaccgagtc tggacgatac ccgcgacgcg agaacccatt
     3121 attggagtgc gttattccgg ccgcggggcc aaaatgcgaa tgtcgcatat cgtccccctc
     3181 tcagaacagt ccatcgccat tctgaaacag attaaggata tcaccggtaa taatgaactg
     3241 atcttccccg gcgaccataa cccgtataag ccaatgtgtg aaaacacggt caataaggca
     3301 ctgcgggtga tgggctacga cacgaaaaag gatatctgcg gtcacggctt ccgggcaatg
     3361 gcatgcagtg cgctgatgga atcgggttta tgggcaaagg acgcagtaga acgccagatg
     3421 agtcatcagg agcgcaatac cgtgcgcatg gcttatattc ataaggcaga gcacctagaa
     3481 gcccgcaaag cgatgatgca gtggtggtcg gattatctgg aagcatgccg agaatcttat
     3541 gcaccgcctt atacaattgg taaaaataag tttatcccat agtatactaa cgaatcccac
     3601 actctggcaa attaccacca cctctacagg aggtggttct atatagactt ttatcaactt
     3661 catgacgaaa aacacctatt ttatccaaaa agaagacaat agagtcacag aggaatcaaa
     3721 gagcgactca attgtctttg gacagtctgg ttgtgaggtt agaaaacagt tactcctaca
     3781 ccattaaata gggcgcaatg ctcgctaatt tctcccgggt ttccgtcagt tcgcgttccg
     3841 gtgtagattg cgcgatgatt cccgcccccg cttgtatcca gcagcgctgg ctgtcctgaa
     3901 atacggaacg cagaactagc gccgcatcga agcgcatatc gtccagcagc agaattgcgc
     3961 cggaatagag ctctcgcggc gtcttctcaa tttgcataat cgcgttcagg gcagcgtttt
     4021 tagggatacc tgaggcggta atcgacggaa acagcacggt aaacgcatcc caggcatcct
     4081 tgttttccgc aagctgaccg ctcacgccgg accccagatg ctgaacgctg ccgcgctggc
     4141 gaaccgacat taaatcttca accaccacgc tgcccggcag gcaaacggcc tccagttcag
     4201 caatcgcttc tttgacagaa aggatatgct caagcacctc tttactgtcg tgcagcagtt
     4261 ctgcctcttt cgccttatta tgctccgggt ttcccatgcg atcgcgggtg cccgcaagcg
     4321 gttcagtgac cactttattg cccgtcactg acatcaccag ttccgggcta aagcccagcg
     4381 cttcgcgtcc ttcctgacgg aacataaacg agcgcacagg tgtatttgcc tgccgcccgt
     4441 ataacagcgt ggcgggcata tcaatccgcg atggcagggg aatagcgcgc gagacaatca
     4501 ctttgacata ctcgccacgg cggatctccg caacggcgcg tgcaacctgt tgtttatacg
     4561 cctcgccatt gagggcggta tccaccgcca gtggtgcgtt ctgcgtcgtt gtactggcct
     4621 cttttaccca ctcacacaaa cgtcggcccc cgtcggcgga gtccgcataa acggtgacat
     4681 ttcccttttc aaaaataagt tcttcacggg gaacggttaa cgtcagcagc ggccactccc
     4741 cggcgttaaa ggcaatcccc cgcgcatgcg ccgcaaagtt gaacccaacc tgtccataaa
     4801 cccgacgtcc tttcacctga ggatgtgcca taaaacgacg cgcgcagtcg gtaatggaat
     4861 ccaccgccca ttgttgctcc ccggcatcat caataaatgt cgcctgcgtt ccgtcggcat
     4921 taatgtgcaa ctgtgccagg caccctttgc ctaaatacca acagggttga cgctcataga
     4981 cataacactc ttcttctgcg aactggcgta aaacgccaga aatcgtcggc agccattgtt
     5041 cctctggtaa cgccaggtgt aaaaattcac tgattttcat cccttacctc tctgtgttat
     5101 tcccggatca atcaattgcg cttgtcatca gttctcttgc cgaatgcagc cggatataag
     5161 caacccacgc cgccagcaac gtgaataccc cggcaaacag gaagcaggcc gcatagccaa
     5221 aatgttgagc aacaacgccg ccgataacac cagccaggat ggcgattgcc gcgtcagtac
     5281 attgaaagag ggtgaagtcg acaccggcct gcaaaggcga tgaaagcgac atcagcgtgg
     5341 cgtaaagaca gaccagcgcg caggccagcg aaatggactg gacaatgacc agacactgga
     5401 gaatcggcag caacagatga cccggcgcca tcatgaacgt catcaccacc gccagcagcg
     5461 cgatcccctg gacgccataa gccgtcaaca gcgctctgcc gggtgaggtg tatttcatca
     5521 gtaatccgcc ggccagcgtt cctgctatgc ccgctgcaat attgccgccg ctgaacagcg
     5581 cgcccaacgc agacatgctc aacccatgat ccaacaacag cggcgccaga agaggcagca
     5641 caaaccgcat acctgaattc agcattaata ccagcagtaa gcccaggcgc gcctgcttcc
     5701 tccttagcgc ataacctaac gccggaacat gcgggatagg cgctgttcgc gtcggctccg
     5761 taatgcgcca cagcgggaat gacagcgcca taatcagcat cgccatcatc agcatggcga
     5821 caggccagcc ggatgctgcc gacaacatta ggaacacccc gccgccgcac atcattccca
     5881 gatagctgcc gccgacctgc tcgctgtttc cccagccgta acccgcgcga gtcagttgat
     5941 cgacgcaaaa gccgtcgctg gcgatatcaa tggtggaagc gaccgtgcct gccaccataa
     6001 acagaacaaa taacgcgacg atttgcgtat cgggaaatcc tccttctcgc ccaaaccagc
     6061 caatcgctgc ggcaatcatc aggatcgccg ctagcgcgac ctgaccacgc aggattaaca
     6121 tccgcgaacg gcgttcctgg ctaccgggcg gaagccgcca gcgttcgatc cacggcgccc
     6181 aaataaactt cagcgcccag ggcagcatga acagggttgt cgcaccggca agcgccagcg
     6241 atccgccagc agcgcgcacc agcgcgggta aggactgcat agacagcgcc gataccagac
     6301 tctgcgtcac atagacgccg gcaatagcaa aaatcacccg cccggtcgtc aacgtcagcg
     6361 gtttcacatt cgactgaaca tcactcatgc acccactctc cagacgcgga aaccccgtcg
     6421 tcgcgccaca cgcgcgcggc catttgcgcc tgccacagcg cctgataacg tccatgatgt
     6481 gagagcaatt gtgcatgagt accctgctca accacctgtc cctcttccat caccagaatg
     6541 tttccggccc cggcgatggt ggataaacgg tgagcgatga taatcaccgt ccggttgtga
     6601 accaggttat cgatcgcttt ttgcaccgcc agctcgcttt caatatccag cgcggcagtc
     6661 ggttcatcga gaatgacgac cggcgcgttt ttcaataacg ctctggcaat ggaaatccgc
     6721 tggcgctcgc cgcccgatag ctggccgccc atctctccca ttggcgtcag ccagccttgc
     6781 ggaagacggg aaataaactc aaggcactgc gccgcgcggg cggcctcttc tacctcctgc
     6841 cgcgtcgcct gtgggcgcgc gatacggata ttcgccagca gcgtgtcatc aaacagccag
     6901 acatcctgga aaacaacaga aatcaggcta ttgagctgtt ccggcgtcag gcggcgaata
     6961 tcgacgccgc caatagaaat ctgcccttgc tgcggatcgg catagcgcat taacagtttg
     7021 gtgaccgtag ttttgcctgc gccggaggca cccaccagcg cgctcatact ggctgccggg
     7081 aacgtcaaag aaacatgatt aagcgcgtgg ccgtcgcctt cttcgtagcg atagctgacg
     7141 ttgtcaaagc ggatatcgta acgttccggc atctcgcttt gctctgcgac gggaagcggt
     7201 gctatcgcca taaaccgctc aatccgttgc agggcgctgg cgatcagttc cacaaccgag
     7261 gtgtagctga taaacatcgc catcggttct gcgaagcgca taatcatcgc gacggcggca
     7321 atcaaaaagg cgaggttcag agtgcccgtc actacccaga cgatcccgga taacaccacc
     7381 acctgtaggc ccaactccac gacgctggcg atcagcatcg tcgcgccagc gccctgacgg
     7441 tgagtgcggg tctgtaagtt ttccagcgca ttgaaatgcg ccagcagcgc ccggctttta
     7501 tcggcatcgc tgccgcaggt gcgcaatacc atcatcccct gagcaaattc aacgatatcg
     7561 ccgctcagac gctggtgcgc ttcccccagc gtctgcattt gtcgccgcat cgccgggcgg
     7621 cgccagtaat aaaacggcac cagcagaggg aagatcagca acatcaccag ccccagccgc
     7681 cagtctatcc acaatgtcgc cagcgacgcc gtcagcggcg tgacaatggt gagcagcaaa
     7741 atattggcta tcgcaataac ataattgagg ttttcatcca cgctgcccag cagcaaggcg
     7801 ttcatttcac ccgccctgcc gcgctggagc ttctccagcg gcacgcggcg taactgctcg
     7861 ccaagtcgca ggcgcaactc atgggtggcc tgcgccagat gaccacggta ttcaaagccc
     7921 aggccatacc agcgtagcac cagcgtcaca attgcggcga cgctgaaggc catagcccag
     7981 ttaagaagtt gcggcgcgtc tccccgtaac agcgcatcaa tgatcggata aagacaggca
     8041 aaggcgatcc cctgcatgaa tgccgccagc aacagcgcca gcatactgcg tcgcagcatc
     8101 ctggcctgac tgccaacgct ggagataagc tggcgccagt tgacgcgcca ggccaggtta
     8161 tccgcaggat tattgtcttt cattttccac cacctcttcc tgtgttccac ccggcaccca
     8221 atgctgcgcc tgctgatagt gttgccagag ccgttgatac aggccgccct gcgccaacag
     8281 ttgcgtatgg ttccccattt ccctgagctg tccgtcggaa aacagcaata tcacatcggc
     8341 ctgagtcacc attgagagac gatgcgcgac catgatgacc gtccggccac gcatggccgc
     8401 cgcgagcgcc ttgataagcg ccgcttcgtt ttccgggtca gcaaacgccg tcgcctcatc
     8461 gagcaccagg atggggcgat cctgcaaaag cgcccgggcg atagtaatgc gctgccgctg
     8521 gccgccggag agaaataccc cacgttcccc gactcgtgtg ttgtaacctt ctggcagagc
     8581 gctaataaaa tcatgggcct gcgccactct ggccgccgct attaccgcct ccagcggcgt
     8641 atccggcgcg cccagacgaa tgttattggc tatcgtgtcg gcaaaaagga agttgtcctg
     8701 agacacaaac gagagttgct tcatcagggt gtccgtctgc atatcacgca gatccacgcc
     8761 tccaatacgg atatggcctt tgtccgggtc ggcgtaacgc agcagcaagc gcgccacggt
     8821 gctttttccg gcgccgcttg gcccgaccag cgccacaatt tgcccggcag gcacatgaaa
     8881 gctcacctcc tgcaacgcgg cgccagtacg cgcttgcgga taatgaaagc tcacctgctc
     8941 aaaggtaatg ctcgcctctt gcggctgctg gtcagactgc ggcagcgata actccggcat
     9001 cgcgagaacc tgataaatac gctgaatgct taaacgcgtt tgcgcgacca ggttattgag
     9061 catcatcatt ggcattacgg cttcggccat tccgctgccc agcagtaaaa cggccaccca
     9121 cgcgataaaa tcgaaactgc catagtgcag caggccgtat cccgaccaga tcaggacaaa
     9181 cagggtcggg agaggattca gaatcgagaa ggaaaaacgc gctgaaaaac cggctttgcg
     9241 ataccaggtt ttgagcacat cgacccactc ttcgagggct cgttgatagc gcaaaaaact
     9301 ggtgctgccg ctatcaaacg ttctcaccac gggcatggcc tgcacgaact caatcaccgc
     9361 cgcgctaacc tgctcgcgcg ccttatgata acgctgagcc atatcttccg aacgctggcg
     9421 ggcgagcacg agaacaaccg atccaaacgc cagtaccccc agcgtcgcga ttgccagccg
     9481 ccagtccagc cagaacaaga taacgattgt cgccagcggc atgatgatcg cgcgggcata
     9541 gagcggcgtg ctgtcggcca caaaaatatg taacgacttc acgtcatcca gcatcacttt
     9601 cgccactgac ccgctgccca tttgctgtaa cacgccaggc ggaagctgca atgctttacg
     9661 ggctaacctg ctgcgcagaa tgttctccag atgaaaagcc gcgtaatgag aggtgttgaa
     9721 cgcctgtaag cgcagaacaa aagttaagac cacgcagccg atcacaccgc ccagcggcca
     9781 ggccggaata gcgtcaggcg tcgcgcgaat gtcacgcagg ctccacgcta gaaaaaggag
     9841 cgcccccagg ctggttaatg ccgccagccc cgccagcgcc atagctaaaa taaccttcct
     9901 gcgaaccggt gtaatcacct gccagagagg aaatcgggac agcgattcag tattggatga
     9961 ttgagatgac atgggagtaa ctgaatttcc tgatgaattt ttacggacgc caaaaacgac
    10021 aattcagggt ggcgttctga gaattaatgt gaataataac cattatcaat aactcatcta
    10081 ccccattcgg ttctatttat acccgcattg gtctaagcca cagggagata accaggtcat
    10141 gacggagtca ccgcaaacgc aatctgaaat ctctattcac cagttggtgg tcgggaaacc
    10201 tgcaaacgat ggcaatattc ctgctcagtg tgaactgctg cgctgttcgt tgcaggaagg
    10261 tatggatatt ttgctctgga ggggccattt tgcgcggcct gagacgttgc agctacacga
    10321 cgatctgggc aggattaact tctcgtgcat cctggaaggc acatcgcgct ttgcgattca
    10381 gggactccgg cgccacaccg actgggagct tgcgcgcaat cgccattaca ttacccatac
    10441 gccagactgt cgcggcagcg cctcctattg tggccgattc gagagcatta ccctctcctt
    10501 cagccctgca accctcgcgc tgtgggtgcc ggatattagc gcggacatta agaataagat
    10561 cgactcccac tgctgttgcc agcagcagcg ttgtaatgct gaaacccact taacggctca
    10621 ggccttgcgt cacgcgctga tgcgcatgca cggcggcttc agtcatgaac aaaagccgtc
    10681 gacgctatgg ttgcaggggc aaagtctggt catgctcagt ctggtgctgg atgaacatcg
    10741 cgaagacgcc agttgcctct cctgccactt caacccgatg gaacgccaga aactgctgcg
    10801 ggcgaaggat ctgctgctgg ctgatctgac gcaagcgccg ggcgtcgcgg agctggccag
    10861 agagtccggt ctgagcgttc tcaaaattaa gcgaggcttc cgcgttctgt tcaacaacag
    10921 cgtctatggc ctgtttcagg ctgaacgaat gcaggaagcc agaagacgcc tggccaacgg
    10981 caatacctcc gtcatgacgg tggcggccga tctgggctat gccaacgcca gccatttctc
    11041 tgccgccttc cagaaacagt ttggcgtaac cccttcgacc tttaaacgcg tgatgtaacc
    11101 ggggtcgcgc ccccctaaac tcctccctga cagaggcccg gataccccat ccgggcctct
    11161 gttctttccg cctgttacca acccgaaacg ggcataaata aacccgttta gggtagcatc
    11221 caattgttaa taattattat tctcatatga gcaatgcttt tcggtaagac gtgccatcag
    11281 gaggaagaat gatttctggc gcaccatctc aggattcgct gttaccagac aaccgccacg
    11341 cggctgatta ccaacaatta cgcgagcggc tcatacagga actgaattta acgccgcagc
    11401 agttacatga agagagcaac ctgatccagg ccggcctgga ttccataaga ttgatgagat
    11461 ggttacactg gtttcgtaaa aatggctacc gccttaccct tcgcgagctg tatgccgccc
    11521 ccacgctggc ggcatggaac cagttaatgc tcagccggtc gcctgagaac gcggaagaag
    11581 aaacgccgcc cgacgaatca tcctggccga acatgaccga aagtaccccc ttcccattga
    11641 cgccagtaca gcacgcctac ctgacgggcc gcatgccggg gcagacgctt ggcggcgtgg
    11701 gttgccacct gtatcaggag tttgaaggcc attgtctgac ggcgtcgcag ctggagcagg
    11761 ccatcacgac cttgctgcaa cgccacccaa tgctgcatat cgcctttcgc cccgacgggc
    11821 agcaggtctg gctaccgcaa ccttactgga acggcgtcac cgttcatgat ttacgccata
    11881 acgacgctga aagccgccag gcctatctgg acgcactgcg ccagcgcctg agccaccgtc
    11941 ttttacgcgt ggaaatcggc gaaacgtttg attttcagct gacgctcttg ccggacaatc
    12001 gccaccgcct ccatgtcaat attgacctgc tgattatgga tgcctccagc tttacgcttt
    12061 tcttcgatga gcttaacgcc ctgctggccg gagaatcgct gccggctatc gacacccgct
    12121 atgatttccg ctcgtatttg ctgcaccagc agaagatcaa tcaaccactg agagacgacg
    12181 cgcgcgctta ctggctggcg aaagcatcga cgcttccccc cgcgcccgtc ttgccgctgg
    12241 cctgcgaacc cgccacgcta cgtgaagtcc gtaatacccg acgccgcatg attgtcccgg
    12301 caacacgctg gcacgccttt agcaaccggg ccggcgagta tggcgtgacg ccgacaatgg
    12361 cgctggcgac ctgtttttct gccgtgctgg ctcgctgggg cggcctgacg cgactgctgc
    12421 ttaacatcac cttattcgac cgccagccgc tgcacccggc ggttggcgcg atgcttgccg
    12481 acttcaccaa tattcttctg ctggataccg cctgcgatgg cgataccgtc agcaacctgg
    12541 cgcgtaaaaa ccagctcacg tttacggagg actgggagca tcgccactgg tccggcgtcg
    12601 aattactccg tgaactcaaa cgccagcagc gctaccccca cggcgccccg gtggtattta
    12661 ccagcaatct ggggcgttcc ctctacagca gccgcgcaga atcgccgttg ggcgagccgg
    12721 aatggggcat ctcgcaaacg ccgcaggtct ggatagatca tctggcgttc gagcatcacg
    12781 gcgaggtctg gctacaatgg gacagcaacg acgcgctgtt ccctccggcg ttagtcgaaa
    12841 cattgttcga cgcctactgc cagttgatta accaactctg cgatgacgaa agcgcctggc
    12901 aaaagccgtt cgcagatatg atgcccgcca gccagcgcgc gatacgcgaa cgggtcaacg
    12961 ccaccggcgc ccccattccc gaaggcttgc tgcatgaagg cattttccgt atcgctctgc
    13021 aacagccgca ggcgctggcg gtaacggaca tgcgttatca gtggaattat catgagctga
    13081 cagactatgc ccgccgttgc gcgggcaggt taatcgagtg cggggttcag cccggcgata
    13141 atgtggctat cacgatgtcg aaaggcgcag gacaacttgt tgcggttctg gccgtcctgc
    13201 tggccggggc ggtttacgtt ccggtttcgc tggatcagcc tgccgcacgg cgcgagaaaa
    13261 tctacgctga cgccagcgtc cggctggtgc tcatttgtca gcacgacgcc agcgccgggt
    13321 cagacgatat tcccgtcctt gcctggcagc aggccattga ggcggagccg atcgccaacc
    13381 cggtagtacg cgcccccacg caaccggcct acattatcta cacctccggc tctaccggta
    13441 cgccgaaagg ggtagtcatt tctcaccggg gagcgcttaa cacctgttgc gatatcaata
    13501 cccgctatca ggttggcccg catgacaggg tgctggccct ctccgcccta cattttgatt
    13561 tatcggttta cgacattttt ggcgtactgc gcgcgggcgg cgcgctggtg atggtgatgg
    13621 aaaatcaacg gcgcgatcct cacgcatggt gtgagctgat ccagcgccat caggtcacgc
    13681 tctggaacag cgtcccggcg ctgttcgata tgctgctgac ctggtgtgaa ggtttcgccg
    13741 acgccacgcc ggaaaacctg cgcgcagtga tgctttccgg cgactggatc gggcttgacc
    13801 tccccgcccg ttatcgggcc ttccggccac aaggacaatt tatcgcgatg ggcggcgcca
    13861 ccgaggcgtc tatctggtct aacgcctgcg aaattcacga cgtccccgcc cactggcgct
    13921 ccatccctta cggttttccg ctaaccaacc aacgctaccg ggtggtggat gaacaggccc
    13981 gggactgccc tgactgggtg ccgggtgaat tatggattgg cggcattggg gtcgcggaag
    14041 gctatttcaa cgatcccctg cgtagcgagc agcaattttt gacgctcccg gacgagcgct
    14101 ggtatcgcac cggcgatctc ggctgctact ggccagatgg cacaatcgag ttcctcggtc
    14161 gtcgcgacaa gcaggtcaaa gtcggaggat atcgcatcga gctgggcgaa atcgaaagcg
    14221 cgctcagcca gctggcgggg gtgaaacaag caaccgttct ggcgatcggc gaaaaagaaa
    14281 aaacgctggc ggcatacgtt gttcctcaag gcgaggcttt ttgcattacc gatcatcgga
    14341 acccggcact gccgcaggcg tggcacacgc ttgcgggaac gttgccctgt tgcgccatct
    14401 cgccagagat ctccgcagaa caggtagccg atttccttca gcatcgcctg ctaaaactga
    14461 agccgggtca caccgctggc gccgatcctc tccccctgat gaactcactc gctatccagc
    14521 cgcgctggca ggccgtggtg gaacgctggt tagcatttct ggtgacacaa cggcgactga
    14581 agcccgctgc tgaaggttat caggtctgcg ctggtgaaga acgcgaggat gagcacccgc
    14641 acttcagcgg acatgattta acgttatcgc aaattcttcg cggtgcccgt aacgaactgt
    14701 cgttactgaa cgacgcgcag tggtcgccgg aaagcctggc ctttaaccat ccggccagcg
    14761 ccccgtatat tcaggaactg gcgacaattt gccaacagct tgcacagcgc ttacagcgcc
    14821 cgatacgcct gcttgaggtg ggaacccgca ctggccgcgc cgcagaatcg ctgttagcac
    14881 agctcaacgc cggacagatt gagtatgtcg ggcttgagca gagccaggag atgctgctga
    14941 gcgcccggca gaggctcgcc ccctggcctg gcgcccgtct gtccctctgg aatgcagaca
    15001 cgcttgcgac gcacgctcac tcggcggaca ttatctggct taataacgcc ctgcatcgtc
    15061 tgctgccgga agatcccggg ctccttgcga cattacaaca gcttgccgtt cccggcgcgc
    15121 tgctctacgt gatggagttt cgccagttaa cgccgtccgc cctgctcagc acgctcctgt
    15181 taaccaatgg gcagccggag gccttgctgc ataacagcgc cgactgggcg gcattattta
    15241 gcgcggccgc cttcaactgt cagcatggcg atgaggtcgc ggggttacaa cgcttcctcg
    15301 tacaatgtcc tgacaggcag gtgcgccgcg atccccgtca acttcaggcc gccctcgctg
    15361 gacgtctgcc ggggtggatg gtgccgcaac ggatcgtctt cctcgacgcc ttaccgctga
    15421 cggctaacgg gaaaattgac taccaggcgc tgaagcgtcg tcatacccct gaagcggaaa
    15481 acccggccga agcggattta ccccagggcg acattgaaaa acaggttgcc gccctctggc
    15541 agcaactctt atcaactggc aatgtcacca gagaaaccga cttcttccag caaggcggcg
    15601 atagcctgct ggcgacccgt ctgaccgggc aacttcatca ggcaggttat gaagcgcaat
    15661 taagcgacct gtttaatcat ccccggctgg cggattttgc cgccacactg cggaaaaccg
    15721 acgtcccggt cgaacaacca ttcgtccact cccctgaaga tcgctaccag ccctttgcgc
    15781 ttaccgacgt gcagcaggct tacctggtgg ggcgtcagcc gggctttgcc ctgggcggcg
    15841 tcggctcaca tttctttgtt gaatttgaaa ttgccgatct ggacctcacc cggctggaga
    15901 cggtctggaa ccgattaatc gcccgccacg atatgctgcg cgccatcgtg cgtgatggac
    15961 agcaacaggt gctcgaacag acgcccccct gggtgatacc cgcacacacc ctccatacgc
    16021 ctgaagaggc gttgcgggtg cgcgaaaaac tggcgcatca ggtactcaac cccgaagtgt
    16081 ggccggtatt cgatctccag gtcggatacg tggacgggat gcctgcccgc ctgtggctgt
    16141 gtctggataa cctgttgctt gacggtctca gcatgcagat cctgctggcg gagctggagc
    16201 acggctaccg ctacccgcaa cagctgcttc cgccgctgcc cgtcaccttc agggattatc
    16261 tgcaacaacc ctcgctacag tcgcccaatc cagattctct ggcatggtgg caggcgcagc
    16321 ttgatgatat tcctccggcg cctgcgttgc cgctgcgctg cttgcctcag gaggttgaaa
    16381 caccgcgctt cgcccgcctg aacggcgcgc tggacagcac gcgctggcat cggctgaaaa
    16441 aacgggcggc tgacgcccat ctcaccccgt cggccgtact gttgtcggtg tggtcaacgg
    16501 ttctctctgc atggagtgca cagcctgagt tcacgcttaa ccttacgctt ttcgacaggc
    16561 gaccgctgca cccgcaaatc aaccagattc tgggcgattt cacctcgctg atgctgctga
    16621 gctggcatcc cggcgaaagc tggctgcaca gcgcgcagtc actacagcag cggctgagcc
    16681 agaacctcaa ccaccgcgat gtgtcagcca tccgcgtgat gcgtcaactg gcgcaacagc
    16741 aaaacgtgcc tgccgttccg atgcccgtcg tctttaccag cgcgctgggc tttgagcagg
    16801 ataacttcct cgcccggcgt aatctgctca aaccggtctg gggcatctcc cagacgccgc
    16861 aggtctggtt cgatcaccag atttatgaat ccgaaggcga actgcgcttt aactgggatt
    16921 ttgtcgccgc gctgtttcct gccgggcagg tggagcgcca gtttgaacag tattgcgcat
    16981 tgctaaaccg aatggccgag gatgaaagcg gctggcaact gccgctcgcc gcgctggtgc
    17041 ctcccgttaa acacgcaggg caatgcgcag agcgctcacc gcgcgtatgc cctgagcaat
    17101 ctcagccaca cattgcggcg gacgagagca ccgtcagcct gatttgcgac gccttccgcg
    17161 aggtggttgg cgagtctgtc acgcccgcag aaaacttctt tgaggcgggc gcaacgtcgc
    17221 tgaatctggt gcaactgcac gttttgttac aacgtcacga attttccacc ctgacgttgc
    17281 ttgacctctt cacccaccct tctcctgcta ccctggccga ttatctggcc ggcgtcacca
    17341 cggtggagaa aacaaaacga cctcgccctg ttcgccgtcg tcagcggcgg atatagcgcg
    17401 aagcaaactg attttccccg gaacgccatc gcgaacgcat ggcgttccat tgactttgtg
    17461 aatcttagga aacgggaccg attatggata acttgcgctt ctcttctgcg ccgacagcag
    17521 attccattga tgcatcgatc gctcaacact acccggactg cgaacctgtc gcggttatcg
    17581 gctacgcctg ccattttcct gaatcgccgg atggcgaaac gttctggcaa aatctgctgg
    17641 aaggtcgtga atgcagccga cgctttacgc gcgaagagct tctggccgtc ggtctggatg
    17701 ccgccatcat tgacgatcct cattatgtca atatcggtac ggtgttagac aacgccgact
    17761 gcttcgacgc caccctgttt ggctattcgc gacaggaagc ggagtcgatg gacccgcagc
    17821 agcgcctatt tttgcaggcg gtctggcatg cgctggaaca tgccggttat gcccccggcg
    17881 ccgtccccca taagaccggc gttttcgcct cttcccggat gagtacctac cccggtcgcg
    17941 aagcattgaa cgtgacagaa gtcgcgcagg taaaaggtct gcaatctctg atgggcaatg
    18001 ataaagacta tattgccacc cgcgccgcgt acaaactcaa cctgcacggc ccggcgttat
    18061 cggtacagac cgcctgctcc agctcgctgg ttgccgtgca tctggcctgt gaaagcctgc
    18121 gcgcaggcga atccgatatg gcggttgccg gcggcgtggc gctctctttc ccccagcagg
    18181 caggctaccg ctaccagccc ggaatgattt tctctcctga tggtcactgt cgtccctttg
    18241 acgcctcggc tgagggcacc tgggccggta acggtctcgg ctgcgtggtg ctgcgtcgcc
    18301 tgagagacgc gctgctgtca ggcgatccga ttatctcggt gatcctctcc agcgcggtca
    18361 acaacgacgg caacagaaag gtcggctata ccgccccttc cgtcgcaggg caacaggcag
    18421 tcatcgaaga ggcgttaatg ctggcggcca tcgacgacag gcaggtaggt tacattgaaa
    18481 cccacggcac cggcacaccg ctgggcgacg cgattgaaat tgaagcgtta cgcaacgtct
    18541 atgcgcctcg cccgcaggat cagcgctgtg cgctcggttc cgtgaaaagt aacatgggcc
    18601 atctggatac cgcggcgggc attgccggac tgctgaaaac cgttctggca gtcagtcgcg
    18661 ggcaaattcc tcccttactg aattttcaca cccccaaccc ggcgctgaaa cttgaagaga
    18721 gcccctttac cataccggtg tcggcacagg catggcagga cgaaatgcgc tatgcgggcg
    18781 tctcctcctt tggtattggc ggcaccaact gccatatgat cgtcgcctcg ctgcccgacg
    18841 cgctcaacgc gcgcctcccc aatacggata gcggcagaaa aagtaccgcg ctgctgctca
    18901 gcgcagccag cgacagcgcg ttgcggcggc tggcgacgga ttatgccggg gcgctgagag
    18961 agaatgcgga tgccagctct ctggccttca cagccctgca cgcgcgccgt ctcgatctcc
    19021 ccttccgcct ggcggcgcca ttaaaccgtg aaaccgccga ggcgctcagc gcctgggccg
    19081 gtgagaaatc gggggcgctg gtttacagcg gccacggcgc cagcggcaag caggtgtggc
    19141 tgtttaccgg ccagggctcg cactggcgca ctatgggtca aacgatgtac cagcactcaa
    19201 cggcgtttgc cgacacgctg gatcgctgtt tttccgcctg tagcgaaatg ctcacgccgt
    19261 cactgcgcga agcgatgttt aaccccgatt cggcgcaact ggacaatatg gcctgggcgc
    19321 agccggcgat tgtcgcgttt gaaatcgcga tggcggcgca ctggcgtgct gaaggactga
    19381 agccagactt cgccattggg cattccgtcg gtgaatttgc cgctgctgtt gtctgcggac
    19441 actatacgat tgaacaggtc atgccactgg tttgtcggcg cggcgcgcta atgcagcagt
    19501 gcgcaagcgg cgcgatggtg gcggtatttg cagacgaaga cacgctgatg ccgctggctc
    19561 gccagtttga gctggatctc gccgccaata acggtacgca acatacggta ttttccggac
    19621 cggaagcccg tctcgcagta ttttgcgcca cgctctcgca gcatgacatt aactatcgtc
    19681 gcctgagcgt aaccggtgcg gcgcactccg ctttactgga gccgatactc gatcggttcc
    19741 aggacgcctg cgcgggactg cacgcggagc cggggcaaat accgattatt tccacgctca
    19801 ccgccgacgt cattgatgag tcaacgctca accaggcgga ttactggcgc cgacacatgc
    19861 gccagccggt gcgttttatc cagagtattc aggtggcgca tcagctcggc gcccgcgttt
    19921 ttctggagat ggggcccgat gcccagttgg ttgcttgcgg gcagcgcgaa taccgcgata
    19981 acgcatactg gatagccagc gcccggcgta acaaagaggc gagcgatgtc ctcaatcagg
    20041 ccctgctcca gctttacgct gccggcgtcg ccctaccgtg ggccgacctg ctggcgggcg
    20101 atggacaacg tatcgctgcg ccatgttatc cgtttgatac tgagcgttac tggaaagagc
    20161 gcgtctcccc ggcctgcgag cctgccgacg cagcgctgtc tgccgggctg gaggtggcga
    20221 gtcgcgccgc gacagcgctc gatctccctc gcctggaagc gcttaaacag tgcgccacgc
    20281 gactgcacgc catctacgtc gatcaactgg tacaacgctg taccggcgat gccattgaga
    20341 acggcgtgga cgccatgacc atcatgcgcc gtggacgtct gctgccccgc taccagcagc
    20401 tactccagcg cctgctgaat aactgcgtgg tcgacggcga ttaccgctgc accgacgggc
    20461 gatacgtccg cgcccgcccc attgaacatc aacagcggga atcactgctg acggaacttg
    20521 ccggttattg tgaaggtttt caggctattc ccgacaccat cgcccgtgcc ggcgatcggt
    20581 tatatgaaat gatgagcggc gcggaagaac cggtggcgat tatcttcccg caaagcgcct
    20641 ccgacggcgt ggaagtgctg tatcaggaat tcagctttgg ccgctatttc aaccaaatcg
    20701 ccgccggggt attacgcggc attgtccaga cgcgtcagcc ccgccagccg ttgcgtattc
    20761 ttgaagttgg cggcggaacc ggcggcacca ccgcgtggct gctgccggaa ctcaacggcg
    20821 ttccggcact ggagtaccat ttcaccgata tctcggcgct gttcacccgt cgcgcccagc
    20881 agaaattcgc cgactatgat tttgtgaagt atagcgagct ggatctcgaa aaagaggcgc
    20941 agtctcaggg tttccaggca cagtcttacg atcttatcgt ggcagcgaac gtgattcacg
    21001 ccacccgcca tattggccgc acgctcgata atctgcgccc cctgctcaag ccgggcgggc
    21061 gcctgctgat gcgcgaaatc acccagccaa tgcgtctgtt tgacttcgtt ttcggcccgc
    21121 tggttcttcc gctacaggat ctcgacgccc gcgaaggtga gttattcctc accaccgctc
    21181 agtggcaaca acagtgccgc cacgccggat tcagcaaagt ggcgtggcta ccgcaggatg
    21241 gcagcccgac cgccgggatg agcgaacata tcattctcgc cacgctgccc ggtcaggcgg
    21301 ttagcgccgt aacattcacc gcgccatcag aacccgtgtt ggggcaggcg ctgacggata
    21361 acggtgatta tctcgccgac tggtctgatt gcgcaggtca gcccgaacgg tttaacgccc
    21421 gctggcagga ggcctggcgt ctgctttcac agcgtcatgg cgacgctctg cctgtggaac
    21481 cgccccccgt cgccgccccg gagtggctgg ggaaggttcg cttaagctgg caaaacgaag
    21541 ccttttcccg cggtcagatg cgcgttgaag cccgtcatcc tgctggcgag tggctgccgc
    21601 tatcgcccgc cgcgcctctt cctgcgccgc agacgcatta tcaatggcgc tggacgcccc
    21661 tcaacgtcgc cagcattgac caaccgctta cctttagctt cagcgccggt acgcttgcgc
    21721 gcagcgacga gctggcgcaa tacggcatca ttcacgatcc gcacgcctct tcgcgactga
    21781 tgattgttga ggagagcgag gatacgctgg ccttagcgga gaaagtgata gcagcgctca
    21841 ccgccagcgc agccggattg attgtggtta ctcgccgcgc gtggcgagtc gaggaaaatg
    21901 aagcactctc tgcatcccat cacgcgctat gggccttgct tcgagtcgcg gccaacgaac
    21961 agccggaacg gttgcttgcc gccatcgatc tcgccgaaaa caccccgtgg gaaacgctgc
    22021 atcaagggtt gagcgcagtc tcactatcac agcgctggct cgccgcacgg ggtgacaccc
    22081 tttggctccc ttcactggcg cccaatacgg gatgcgccgc tgaattaccg gcaaacgtgt
    22141 ttaccggcga tagccgctgg catctggtga ccggagcgtt tggcggatta ggccgccttg
    22201 ccgtgaactg gctcagagaa aaaggggcgc gacgcatcgc cctgctggcg ccgcgcgtgg
    22261 atgattcatg gctacgcgac gtggagggcg ggcagacgcg cgtctgccgt tgtgatgtgg
    22321 gcgatgccgg gcaactggcc acggttcttg acgatctggc ggccaacggc ggcattgccg
    22381 gagcgattca tgccgctggc gtattggctg acgcgccctt gcaggagctt gatgaccacc
    22441 agctggctgc cgttttcgcg gtaaaagcgc aggcggcaag ccagctgttg caaaccctgc
    22501 gcaaccacga cggacgctat cttattctct actcttccgc tgccgccacc ctcggcgcgc
    22561 cgggtcagag cgcccatgcg ctggcctgcg gctacctgga cgggctggcc cagcagtttt
    22621 ccacccttga tgcgccgaaa acgctctctg tcgcctgggg cgcatgggga gaaagcggtc
    22681 gggcggccac gccggaaatg ctggcgacgc tcgccagccg aggtatgggc gcgttaagcg
    22741 atgccgaagg ctgctggcac ctggaacagg cggtgatgcg cggcgccccg tggcgactgg
    22801 cgatgcgcgt ttttaccgac aaaatgcccc cgttacaaca ggctctgttt aacatcagcg
    22861 ccacagaaaa agccgcaacg ccggtcattc ctcctgctga tgacaacgcc tttaacggca
    22921 gcctgagcga tgaaacagcg gtgatggcat ggctgaaaaa gcggattgcg gttcagctaa
    22981 ggctgagcga tccggcgtca ctgcatccaa accaggatct gttgcaactc ggcatggact
    23041 cgctgctctt ccttgaactc agtagcgata ttcagcactt cctgggcgta cgcatcaatg
    23101 cggaacgggc gtggcaggat ctgtctcctc atggactcac gcagcttatc tgttctaagc
    23161 cagaggcgac gcctgccgct tcgcagccgg aagtgttgcg gcacgacgcc gacgagcgtt
    23221 atgcgccctt ccctttgacg cccattcagc acgcctactg gctggggcga acccacctca
    23281 ttggctatgg cggcgtcgcc tgtcacgtcc tgtttgagtg ggataaacgc cacgatgagt
    23341 tcaatctcgc catactggag aaagcatgga accagctcat cgcacgccac gatatgttgc
    23401 gtatggtggt tgatgccgac gggcagcagc gaatcctggc gacaacgccg gagtatcaca
    23461 tcccgcgtga cgatctgcgc gcgctttccc cggaagaaca gcgcatcgcg ctggaaaaac
    23521 ggcggcatga actgagctat cgcgttttgc ctgccgacca gtggcctctt tttgagctgg
    23581 tggtcagcga aatcgacgat tgccattacc gtctgcatat gaacctcgac cttttgcagt
    23641 ttgatgtgca gagttttaaa gtcatgatgg acgacctggc gcaggtctgg cgcggtgaaa
    23701 cgctggcgcc gctcgctatt accttccgtg attatgtgat ggctgaacag gcgcgccgac
    23761 agacatcggc atggcacgat gcctgggatt actggcagga aaaactgccg caactgccct
    23821 tagcgccaga gctgccggtg gttgagacgc ccccggaaac gccacacttc accaccttca
    23881 aatcgacgat cggcaagaca gaatggcagg ccgtgaaaca gcgctggcag cagcaaggcg
    23941 tcacaccgtc tgccgcgctg ctcacgctgt ttgccgccac ccttgagcgc tggagccgta
    24001 ccacaacatt tacgctgaac ctgacgttct tcaatcgcca gccgatccat ccgcaaatca
    24061 accagttgat tggtgatttt acctccgtca cgctggttga ttttaacttc tcagcgccgg
    24121 tgacgttgca agagcagatg caacagaccc aacagcgcct ctggcaaaac atggcgcaca
    24181 gtgaaatgaa cggtgttgag gtgatccgtg agctgggccg cctgcgcgga tcacaacgtc
    24241 aaccgctgat gccggtagtg tttaccagta tgctggggat gacgctggaa ggcatgacta
    24301 tcgatcaggc gatgagccat ctgttcggcg aaccctgcta tgtattcacg caaacgccgc
    24361 aggtctggct ggatcatcag gtcatggaga gcgacggcga gttgatgttt agctggtact
    24421 gcatggacaa cgtgctggaa cccggcgctg ccgaggcgat gtttaatgac tattgcgcca
    24481 tcctgcaagc cgtcatcgcc gcccctgaaa gcctgaagac tctcgccagc ggcatcgccg
    24541 ggcacattcc ccgccgacgc tggccgctga acgcgcaggc ggactacgac ctgcgggata
    24601 ttgagcaggc gacgctcgaa taccccggca tccggcaggc cagagcggaa ataaccgaac
    24661 agggcgcgtt gacgctggat atcgtgatgg ccgacgatcc gtcgccatca gcggcgatgc
    24721 ctgatgagca cgaacttacc caactggcgc tgccgttgcc tgagcaggcg cagcttgatg
    24781 agctggaggc gacctggcgc tggctggagg cgcgtgcgct acaggggatc gcggctacgc
    24841 taaatcgtca cggcctgttt accacgccgg agatcgccca tcgctttagc gcaatagtac
    24901 aggcgctgtc cgcgcaagcg tctcaccagc gtctgctgcg ccagtggcta cagtgtctga
    24961 cggaaagaga gtggttaatc cgcgaaggtg aaagctggcg ctgccgcatt ccgctcagcg
    25021 agattcctga gcctcaggaa gcgtgcccgc aaagccaatg gagccaggcg ctggcgcagt
    25081 atctggaaac ctgcatcgcc cggcacgacg ccctcttctc cgggcggtgt tctccgctgg
    25141 aattgctgtt caacgagcag catcgcgtta ccgacgcgct gtatcgcgac aaccccgcca
    25201 gcgcctgtct gaatcgctat accgcgcaga ttgccgcctt gtgcagcgca gaacggattc
    25261 tggagattgg cgccggaacc gcagccacta ccgcgccggt gctgaaggcc acgcggaaca
    25321 cgcggcagtc gtaccacttc acggacgtct ccgcgcagtt cctcaatgac gccagagccc
    25381 gtttccatga tgaatcgcag gtgtcttatg ccttgttcga catcaaccag ccgctggatt
    25441 tcaccgccca cccggaggcg ggttacgacc tgatcgttgc cgtcaatgtg ctccacgacg
    25501 ccagccatgt cgtccagacg ttgcgcagat taaaactgtt gctgaaagcc ggcggacgtt
    25561 tgctgatcgt tgaagcgacg gagcgaaaca gcgtattcca gctggcgagc gtgggcttta
    25621 ttgagggatt aagcggatac cgcgatttcc gccgccggga tgagaaaccg atgctcaccc
    25681 gctccgcatg gcaggaggtt ctcgttcagg ccgggtttgc aaacgagctg gcgtggcccg
    25741 cgcaggaatc gtcgccgctg cgccagcatc tgctggtggc gcgttcgcct ggcgtaaatc
    25801 gcccggataa aaaagccgtg agccgctatt tacagcagcg ctttggcacc ggtctgccca
    25861 ttttacagat ccggcaaaga gaagcgttat ttacgccgct gcatgccccg tctgatacgc
    25921 cgactgagcc agccaaaccc acgccagttg ccggggggaa tccggcgctg gaaagacagg
    25981 tggctgaact ctggcaatcg ctgctgtctc gccccgtggc aaggcatcac gactttttcg
    26041 aactgggtgg cgacagcctg atggcgacaa ggatggtcgc gcagctaaac cggagaggga
    26101 ttgctagggc taaccttcag gatctgttca gccattcgac gctgagcgac ttctgcgccc
    26161 atctacaggc ggctacgtca ggagaggaca acccgatacc cctttgccag ggcgacggtg
    26221 aggaaaccct gtttgtcttc cacgcttcag acggcgatat cagcgcctgg ctgccgctcg
    26281 ctagcgcgtt gaacaggcgc gttttcggcc tgaaagcaaa atcgccgcag cgctttgcca
    26341 cgctcgacca gatgatcgat gagtatgtcg ggtgcatccg tcgtcagcag cctcacggcc
    26401 cttatgtgct ggcgggttgg tcgtatggcg cgtttcttgc ggcgggcgcc gcacagcgcc
    26461 tgtacgccaa aggcgagcag gttcggatgg tgttaatcga tcccgtgtgc cgacaggatt
    26521 tctgttgcga aaaccgggcg gccctgctgc gcctgttagc cgaaggacaa acgcctctgg
    26581 cactgcccga acatttcgac cagcagacgc ccgacagcca gcttgccgac tttatcagcc
    26641 tcgctaaaac ggccggtatg gtgtcgcaaa acctgacgct gcaagcggca gaaacgtggc
    26701 tcgacaacat cgcgcatctg ctgcgtttac tgactgagca tacgccgggc gaaagcgttc
    26761 cggtcccctg tctcatggtg tatgccgccg ggagacccgc gcgctggacg ccagcagaaa
    26821 ccgagtggca gggctggata aacaacgccg acgacgctgt gattgaagcc agccactggc
    26881 aaatcatgat ggaagccccc cacgttcagg cttgtgcgca acacattacg cgctggcttt
    26941 gcgcaacctc aacgcaaccg gagaacacgt tatgatgccg tccgcctccc caaaacaacg
    27001 cgtactgatt gtgggcgcca aatttggcga aatgtacctg aatgccttta tgcagccccc
    27061 ggaggggctg gaactggtcg gcctgctggc gcagggaagc gcccgttcaa gagagctggc
    27121 tcatgcgttt ggcattccgc tgtatacctc gccggaacag ataaccagga tgccggatat
    27181 cgcctgtatt gtcgtgcgtt cgaccgtcgc cggaggaacc ggtacgcagc ttgccagaca
    27241 ctttctgacg cgcggcgtac acgtcattca ggagcatccc ctccacccgg atgacatcag
    27301 ttccttacaa acgctggcgc aggaacaggg ctgctgctat tgggtaaaca cgttttatcc
    27361 gcatacgcgc gcgggacgca catggcttcg cgatgctcag cagctgcgtc gctgcctggc
    27421 taagacgccg ccggtagtcc acgccaccac cagccgacag ttgctcaact ccactctgga
    27481 tttgttgttg ctggctctgg gcgttgatgc cgccgccgtg gagtgcgacg tggttggcag
    27541 ctttagcgat tttcactgtc tgagactctt ctggccggag ggagaagcct gcctgctgct
    27601 tcagcgctat ctcgatcctg acgatccaga tatgcatagc cttatcatgc accgcctgct
    27661 gctgggctgg ccggaaggtc atctttcgct ggaggcgagc tacggccccg tcatctggtc
    27721 atccagcctg tttgtcgcag accatcagga gaacgcccac agcctctacc gcagaccgga
    27781 gatcctgcgc gatctgctgg gtctgacgcg cagcgcggcg cccctgagct ggcgcgactg
    27841 ctgcgaaacc gtcgggccgg agggcgtcag ctggttgcta caccagttgc gcagccatct
    27901 ggcgggcgaa catccaccag cggcctgcca aagcgtacat cagatcgcgt tatcacgttt
    27961 atggcagcag attttacgta agaccggcaa tgcggagatc agacgcctga caccgccgca
    28021 ccacgaccgg ttagccggtt tctacaacga tgatgataag gaggcgctgt gacgcaatct
    28081 gcaatgtgca tcccgctgtg gcccgcccgg aacggcaata ctgcgcatct ggtcatgtgc
    28141 cctttcgctg gcggcagcag tagcgcgttt cgccactggc aagctgagca actggctgat
    28201 tgcgcgcttt ctctggtgac ctggccgggg cgcgatcgcc ttcgccatct ggaaccgctc
    28261 agaagcatta cacaactggc ggcactgctg gcgaacgagc tggaagcatc cgtatcgcct
    28321 gacacgccgc ttttactcgc cgggcacagc atgggggcgc aggtggcgtt tgaaacctgc
    28381 cgacttctgg agcaacgggg gcttgcgcca caaggactga ttatttccgg gtgccatgcc
    28441 ccgcatctgc attctgaacg ccagctcagc catcgcgatg atgccgactt tatcgctgag
    28501 ctgatagaca ttggcggatg ttctcctgaa ctgcaggaaa accaggaatt aatgtcgctg
    28561 tttcttcctc ttctgcgcgc tgatttttac gccaccgaga gctatcacta cgactcgccc
    28621 gacgtctgtc cgccgctgcg cacgcctgcg ctgttattgt gcggcagcca cgatcgcgaa
    28681 gcctcctggc agcaggtcga tgcctggcgt cagtggctga gccacgttac aggcccggtg
    28741 gtgattgacg gcaatcattt ctatcccatt caacaagccc ggtccttttt tacgcagatt
    28801 gtccgccatt ttccccacgc attttctgca atgaccgcgt tgcaaaaaca gcccagtact
    28861 tcagaaaggt gacgcatgaa ttcttccttt aaatctctga ttgaacagta tcccttaccc
    28921 attgccgaac agttgcgcca ctgggcggcc cgttatgcct cgcgaattgc cgtcgttgat
    28981 gcaaaggggt cgttaaccta cagcgcgctt gatgcacaag ttgacgaact tgccgcaggt
    29041 ctgtcatcac tgggtttgcg ttcgggggag catgtaattg tgcagcttcc caacgacaac
    29101 gcgtttgtta ccctgctgtt ctccttgtta agactgggcg ttatccccgt gctggcgatg
    29161 ccctcgcaac ggccgctgga tatcgacgcg ctgattgagc tggcgcaacc cgtcgcttac
    29221 gttattcacg gggaaaacca cgcagagctg gcccgacaga tggcgcacaa acacgcctgc
    29281 ttgcgtcatg ttctggtcgc tggagagacc gtgagcgacg attttacgcc gctcttctcc
    29341 cttcacggtg agcgacaggc atggccgcag cctgatgttt ccgccaccgc gttgttgttg
    29401 ctctcaggcg gcacaaccgg cacgcccaaa ctcatcccgc gccgacatgc cgactatagc
    29461 tataacttca gcgcttctgc tgaactgtgc ggcatcagcc aacagagcgt gtatctcgcc
    29521 gtcctcccgg tggcgcataa ctttccgctg gcctgccccg gtattctggg aacgcttgcc
    29581 tgcggcggaa aagtggtgct gaccgacagc gccagctgtg atgaggtgat gcctttaatc
    29641 gcgcaggaaa gagtgactca cgtcgccctg gttccggcgc tggcgcaatt atgggtgcag
    29701 gccagggagt gggaagacag cgacctttcg tcgctgcgcg tcattcaggc aggcggcgcc
    29761 cggctcgacc cgacgcttgc tgagcaggtt atcgccacct ttgactgtac cctgcaacag
    29821 gttttcggta tggcggaagg cctgctctgt tttacccggc tggacgatcc gcatgccacc
    29881 attctccaca gccaggggcg cccgttgtcc cctctggatg aaatccgcat cgttgatcaa
    29941 gacgagaacg acgtcgcgcc gggcgaaacc gggcaattgt taacgcgcgg cccttatacc
    30001 atttcgggct attaccgcgc ccctgcccac aacgcgcagg cctttaccgc gcaagggttt
    30061 taccgcacag gcgacaatgt caggctggat gaggtaggga acctgcacgt tgagggacgc
    30121 ataaaagagc agatcaaccg cgccggagaa aaaatagccg cggctgaagt ggaatcggca
    30181 ctgctgcgtt tagcggaagt gcaagattgc gcggtggtcg ccgcgccgga cacgctgctt
    30241 ggcgagcgga tttgcgcgtt tatcatcgcg cagcaggtgc caactgacta tcagcagttg
    30301 cgtcaacaac tgacccgtat ggggctcagc gcgtggaaaa ttcctgacca aatcgagttt
    30361 ctggaccact ggccgctcac cgccgtcggc aagatagaca aaaaacgcct gacggctctc
    30421 gccgtcgacc attatcgcca ttctgcccaa taagcgcaaa ccgacccgaa acaggttgaa
    30481 ataaacccgt ttcgggtagc accactatta gaaatagtta tcattttcaa ttcaccattg
    30541 tcggtatttt tggcgtttcg ccgtcttaca gggactcaca acaatgaaaa tgacacggct
    30601 ttatcctctg gccttggggg gattattgct ccccgccatt gctaatgccc agacttcaca
    30661 gcaagacgaa agcacgctgg tggttaccgc cagtaaacaa tcttcccgct cggcatcagc
    30721 caacaacgtc tcgtctactg ttgtcagcgc gccggaatta agcgacgccg gcgtcaccgc
    30781 cagcgacaaa ctccccagag tcttgcccgg gctcaatatt gaaaatagcg gcaacatgct
    30841 tttttcgacg atctcgctac gcggcgtctc ttcagcgcag gacttctata accccgccgt
    30901 caccctgtat gtcgatggcg tccctcagct ttccaccaac accatccagg cgcttaccga
    30961 tgtgcaaagc gtggagttgc tgcgaggccc acagggaacg ttatatggca aaagcgctca
    31021 gggcgggatc atcaacatcg tcacccagca gccggacagc acgccgcgcg gctatattga
    31081 aggcggcgtc agtagccgcg acagttatcg aagtaagttc aacctgagcg gccccattca
    31141 ggatggcctg ctgtacggca gcgtcaccct gttacgccag gttgatgacg gcgacatgat
    31201 taaccccgcg acgggaagcg atgacttagg cggcacccgc gccagcatag ggaatgtgaa
    31261 actgcgtctg gcgccggacg atcagccctg ggaaatgggc tttgccgcct cacgcgaatg
    31321 tacccgcgcc acccaggacg cctatgtggg atggaatgat attaagggcc gtaagctgtc
    31381 gatcagcgat ggttcaccag acccgtacat gcggcgctgc actgacagcc agaccctgag
    31441 tgggaaatac accaccgatg actgggtttt caacctgatc agcgcctggc agcagcagca
    31501 ttattcgcgc accttccctt ccggttcgtt aatcgtcaat atgcctcagc gctggaatca
    31561 ggatgtgcag gagctgcgcg ccgcaaccct gggcgatgcg cgtaccgttg atatggtgtt
    31621 tgggctgtac cggcagaaca cccgcgagaa gttaaattca gcctacgaca tgccgacaat
    31681 gccttattta agcagtaccg gctataccac cgctgaaacg ctggccgcat acagtgacct
    31741 gacctggcat ttaaccgatc gttttgatat cggcggcggc gtgcgcttct cgcatgataa
    31801 atccagtaca caatatcacg gcagcatgct cggcaacccg tttggcgacc agggtaagag
    31861 caatgacgat caggtgctcg ggcagctatc cgcaggctat atgctgaccg atgactggag
    31921 agtgtatacc cgtgtagccc agggatataa accttccggg tacaacatcg tgcctactgc
    31981 gggtcttgat gccaaaccgt tcgtcgccga gaaatccatc aactatgaac ttggcacccg
    32041 ctacgaaacc gctgacgtca cgctgcaagc cgcgacgttt tatacccaca ccaaagacat
    32101 gcagctttac tctggcccgg tcgggatgca gacattaagc aatgcgggta aagccgacgc
    32161 caccggcgtt gagcttgaag cgaagtggcg gtttgcgcca ggctggtcat gggatatcaa
    32221 tggcaacgtg atccgttccg aattcaccaa tgacagtgag ttgtatcacg gtaaccgggt
    32281 gccgttcgta ccacgttatg gcgcgggaag cagcgtgaac ggcgtgattg atacgcgcta
    32341 tggcgcactg atgccccgac tggcggttaa tctggtcggg ccgcattatt tcgatggcga
    32401 caaccagttg cggcaaggca cctatgccac cctggacagc agcctgggct ggcaggcgac
    32461 tgaacggatg aacatttccg tctatgtcga taacctgttc gaccgtcgtt accgtaccta
    32521 tggctacatg aacggcagca gcgccgtcgc gcaggtcaat atgggtcgca ccgtcggtat
    32581 caatacgcga attgatttct tctgattatt gtaaaaggga taccgaaaag gtatcccttt
    32641 tacaccacta gttaaaacca gtaactcagc agagtcgcaa aaaatattaa tcc
//
LOCUS       DAFNBJ010000039        32303 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000039 DAFNBJ010000000
VERSION     DAFNBJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.039"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(63..1823)
                     /locus_tag="L2X49_001741"
     CDS             complement(63..1823)
                     /locus_tag="L2X49_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HBN4796836.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1953..2645
                     /locus_tag="L2X49_001742"
     CDS             1953..2645
                     /locus_tag="L2X49_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBN4796837.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2844..3932
                     /gene="serC"
                     /locus_tag="L2X49_001743"
     CDS             2844..3932
                     /gene="serC"
                     /locus_tag="L2X49_001743"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBN4796838.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4003..5286
                     /gene="aroA"
                     /locus_tag="L2X49_001744"
     CDS             4003..5286
                     /gene="aroA"
                     /locus_tag="L2X49_001744"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBN4796839.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5455..6219
                     /gene="ycaL"
                     /locus_tag="L2X49_001745"
     CDS             5455..6219
                     /gene="ycaL"
                     /locus_tag="L2X49_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBN4796840.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6392..7075
                     /gene="cmk"
                     /locus_tag="L2X49_001746"
     CDS             6392..7075
                     /gene="cmk"
                     /locus_tag="L2X49_001746"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBN4796841.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7186..8859
                     /gene="rpsA"
                     /locus_tag="L2X49_001747"
     CDS             7186..8859
                     /gene="rpsA"
                     /locus_tag="L2X49_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBN4796842.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9019..9303
                     /gene="ihfB"
                     /locus_tag="L2X49_001748"
     CDS             9019..9303
                     /gene="ihfB"
                     /locus_tag="L2X49_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBN4796843.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9510..11774
                     /locus_tag="L2X49_001749"
     CDS             9510..11774
                     /locus_tag="L2X49_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HBN4796844.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPSLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHCISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11811..13559
                     /gene="msbA"
                     /locus_tag="L2X49_001750"
     CDS             11811..13559
                     /gene="msbA"
                     /locus_tag="L2X49_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBN4796845.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13556..14542
                     /gene="lpxK"
                     /locus_tag="L2X49_001751"
     CDS             13556..14542
                     /gene="lpxK"
                     /locus_tag="L2X49_001751"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBN4796846.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14579..15811
                     /locus_tag="L2X49_001752"
     CDS             14579..15811
                     /locus_tag="L2X49_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBN4796847.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15863..16045
                     /gene="ycaR"
                     /locus_tag="L2X49_001753"
     CDS             15863..16045
                     /gene="ycaR"
                     /locus_tag="L2X49_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBN4796848.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16042..16788
                     /gene="kdsB"
                     /locus_tag="L2X49_001754"
     CDS             16042..16788
                     /gene="kdsB"
                     /locus_tag="L2X49_001754"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBN4796849.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIRNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16942..17835
                     /locus_tag="L2X49_001755"
     CDS             16942..17835
                     /locus_tag="L2X49_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBN4796850.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17812..18591)
                     /gene="elyC"
                     /locus_tag="L2X49_001756"
     CDS             complement(17812..18591)
                     /gene="elyC"
                     /locus_tag="L2X49_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBN4796851.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18727..19512
                     /gene="cmoM"
                     /locus_tag="L2X49_001757"
     CDS             18727..19512
                     /gene="cmoM"
                     /locus_tag="L2X49_001757"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBN4796852.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19509..20831
                     /gene="mukF"
                     /locus_tag="L2X49_001758"
     CDS             19509..20831
                     /gene="mukF"
                     /locus_tag="L2X49_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBN4796853.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20812..21516
                     /gene="mukE"
                     /locus_tag="L2X49_001759"
     CDS             20812..21516
                     /gene="mukE"
                     /locus_tag="L2X49_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBN4796854.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21516..25976
                     /gene="mukB"
                     /locus_tag="L2X49_001760"
     CDS             21516..25976
                     /gene="mukB"
                     /locus_tag="L2X49_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBN4796855.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26237..28084
                     /gene="ldtD"
                     /locus_tag="L2X49_001761"
     CDS             26237..28084
                     /gene="ldtD"
                     /locus_tag="L2X49_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBN4796856.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAILAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMVKQMTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28265..28813
                     /locus_tag="L2X49_001762"
     CDS             28265..28813
                     /locus_tag="L2X49_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBN4796857.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28840..29487
                     /gene="gloC"
                     /locus_tag="L2X49_001763"
     CDS             28840..29487
                     /gene="gloC"
                     /locus_tag="L2X49_001763"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBN4796858.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29709..30899)
                     /gene="aspC"
                     /locus_tag="L2X49_001764"
     CDS             complement(29709..30899)
                     /gene="aspC"
                     /locus_tag="L2X49_001764"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBN4796859.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31084..32172)
                     /gene="ompF"
                     /locus_tag="L2X49_001765"
     CDS             complement(31084..32172)
                     /gene="ompF"
                     /locus_tag="L2X49_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBN4796860.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7845 a         7856 c         8704 g         7898 t
ORIGIN      
        1 aaaacgcccc gtaatataac tcagactaat cattaagcct acattgcgta ggctatttga
       61 ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc cttcaacaac
      121 gactgatgtg cagcaaacgc gtgcagatcg ctatctaccg gttgcaggcc gtaaaacgcc
      181 gcttcgccgc tcattgccgc actggcggct tctacggcat ctgcgccgta catgcgaaca
      241 aacgcattca gatattgcag cggctggcga tcttcttcct gtgccagtaa taacaacgtt
      301 tgcaggcagc gataatagtt ggcgcgttcc ggactaaata ctgatgagtt aaactccatc
      361 gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc cagcatggct
      421 tttaattcac cgatacgcag ggtgtaccaa ccgttatccg acccggtcgc cagacccaac
      481 agctcacgca cgcgggtaaa gtcatcaaaa ccttcttcat ccagttgctc gatgaggttc
      541 aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc acgtaaatgg
      601 ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc ggacatgcca
      661 ggcacgataa tgcggcaagc gtagacaccc agatgctcgt aatcggcaat ataaacttct
      721 ttatcttcct ttttgaagat agccatcagc gtggcgaact cttcttccgt agtgccggag
      781 aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca ggagattaaa
      841 ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc ttcatcatcg
      901 aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag cagctcggtc
      961 acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc aaagcaggta
     1021 ccgttagcag gattgaacag taccacgcaa atcaccggat actggccgcc aagcgaaccg
     1081 tcataagcga agattgggaa accctccgct tccagcgttt cgatggcttc aactactgct
     1141 gggtaacgcg ccagcacgtc tgccgggatc tctggcaggc tgatgctttc agcaataatg
     1201 cggtttttca cgtagcgttc gaaaacttca gacaatccct gaacgcgtgc ttcgttgcgg
     1261 gtattacctg cggacatacc gttggagacg tacaggttac caatgatatt catcggaata
     1321 taaacggtct gattgtcgga ctgacgcgta aacggcaggc cgcaaatacc acgatcttcg
     1381 ttaccggatt gtaggtcaat cagcatgctg ccggtcagtt cattctccgg atcataaaac
     1441 gcgcgcagac ggtcatcgag tagtccttct ggcacatcgt cattttcggt cagtgggaac
     1501 catttttcgt tgggataatg cacgaacgga ccgttggcga tggtttcgcc cagccagaaa
     1561 tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccaagtgc agaagccagc
     1621 gccgcttttt tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc tttgtcgcga
     1681 atatgtacag accagacgtt aggcacagga ttcagccagg aggcctcttc aatctgaaag
     1741 ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagagc ggcatctttg
     1801 ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc gcgcaataat
     1861 acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt tgactatgct
     1921 tttagcggat aaaccacgat aagagcataa aaatgaaggc gttcgatctc caccgtatgg
     1981 catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt ctttatacct
     2041 ttgtactggt ttttttgttc ctcaaaatga ccggaagacg cggtgtgcga cagatgtcgc
     2101 tgtttgaagt gttaatcatt ctcacgctgg gttcagcggc gggagatgtg gcgttttatg
     2161 atgatgtgcc gatggtgccg gtacttatcg tctttatcac tctggcgttg ttataccgcc
     2221 tggtaatgtg gttgatggcg cacagtgaaa aactggaaga tttactggaa ggtaagccgg
     2281 ttgttattat tgaagatggc gagctggcct ggtcgaaact caataactcc aacatgacgg
     2341 aatttgagtt ctttatggag ctacgattgc gtggcgtgga gcagctgggg caggtacgtc
     2401 tggcgattct cgaaaccaac gggcaaatca gtgtctattt ctttgaagat gacaaggtga
     2461 aaccgggttt acttatttta cccagtgatt gtacgcagcg ttacaaagtg gtgccggagt
     2521 cggcggacta tgcctgtatt cgttgtagtg aaatcattca tatgaaagcg ggggaaaaac
     2581 aattatgtcc gcgctgtgca aatccagaat ggacgaaggc aagtcgggca aaacgggtga
     2641 cctgacagta aaaacatcgg ctttttgcta ataatccgag agattctttt gtgtgatgca
     2701 agccacattt ttgccctcaa cggttttact cattgcgatg tgtgtcactg aatgataaaa
     2761 ccgatagcca caggaataat gtattacctg tggtcgcaat cgattgaccg cgggttaata
     2821 gcaacgcaac gtggtgaggg gaaatggctc aaatcttcaa ttttagttct ggtccggcaa
     2881 tgctaccggc agaggtgctt aaacaggctc aacaggaact gcgcgactgg aacggtcttg
     2941 gtacgtcggt gatggaagtg agtcaccgtg gcaaagagtt cattcaggtt gcagaggaag
     3001 ccgagaagga ttttcgcgat cttcttaatg tcccctccaa ctacaaggta ttattctgcc
     3061 atggcggtgg tcgcggtcag tttgctgcgg taccgctgaa tattctcggt gataaaacca
     3121 ccgcagatta tgttgatgcc ggttactggg cggcaagtgc cattaaagaa gcgaaaaaat
     3181 actgcacgcc taatgtcttt gacgccaaag tgactgttga tggtctgcgc gcggttaagc
     3241 caatgcgtga atggcaactc tctgataatg ctgcttatat gcattactgc ccgaatgaaa
     3301 ccatcgacgg tatcgccatc gacgaaacgc cagacttcgg caaagatgtg gtggtcgccg
     3361 ccgacttctc ttcaaccatt ctttcccgtc cgattgacgt cagccgttat ggcgtgattt
     3421 acgctggcgc gcagaaaaat atcggcccgg ctggcctgac aatcgtcatc gttcgtgaag
     3481 atttgctggg caaagcgaat atcgcgtgtc cgtcgattct ggattattcc atcctcaacg
     3541 ataacgactc catgtttaac acgccgccga catttgcctg gtatctatct ggtctggtct
     3601 ttaaatggct gaaagcgaac ggcggtgtag ctgaaatgga taaaatcaat cagcaaaaag
     3661 cagaactgct atatggggtg attgataaca gcgatttcta ccgcaatgac gtggcgaaag
     3721 ctaaccgttc gcggatgaac gtgccgttcc agttggcgga cagtgcgctt gacaaattgt
     3781 tccttgaaga gtcttttgct gctggccttc atgcactgaa aggtcaccgt gtggtcggcg
     3841 gaatgcgcgc ttctatttat aacgccatgc cgctggaagg cgttaaagcg ctgacagact
     3901 tcatggttga gttcgaacgc cgtcacggtt aatgccgaaa ttttgcttaa tccccacagc
     3961 cagcctgtgg ggtttttatt tctgttgtag agagttgagt tcatggaatc cctgacgtta
     4021 caacccatcg ctcgtgtcga tggcactatt aatctgcccg gttccaagag cgtttctaac
     4081 cgcgctttat tgctggcggc attagcacac ggcaaaacag tattaaccaa tctgctggat
     4141 agcgatgacg tgcgccatat gctgaatgca ttaacagcgt taggggtaag ctatacgctt
     4201 tcagccgatc gtacgcgttg cgaaattatc ggtaacggcg gtccattaca cgcagaaggt
     4261 gccctggagt tgttcctcgg taacgccgga acggcaatgc gtccgctggc ggcagctctt
     4321 tgtctgggta gcaatgatat tgtgctgacc ggtgagccgc gtatgaaaga acgcccgatt
     4381 ggtcatctgg tggatgcgct gcgcctgggc ggggcgaaga tcacttacct ggaacaagaa
     4441 aattatccgc cgttgcgttt acagggcggc tttactggcg gcaacgttga cgttgatggc
     4501 tccgtttcca gccaattcct caccgcactg ttaatgactg cgcctcttgc gccggaagat
     4561 acggtgattc gtattaaagg cgatctggtt tctaaacctt atatcgacat cacactcaat
     4621 ctgatgaaga cgtttggtgt tgaaattgaa aatcagcact atcaacaatt tgtcgtaaaa
     4681 ggcgggcagt cttatcagtc tccgggtact tatttggtcg aaggcgatgc atcttcggct
     4741 tcttactttc tggcagcagc agcaatcaaa ggcggcactg taaaagtgac cggtattgga
     4801 cgtaacagta tgcagggtga tattcgcttt gctgatgtgc tggaaaaaat gggcgcgacc
     4861 atttgctggg gcgatgatta tatttcctgc acgcgtggtg aactgaacgc tattgatatg
     4921 gatatgaacc atattcccga tgcggcgatg accattgcca cggcggcgtt atttgcaaaa
     4981 ggcaccacca cgctgcgcaa tatctataac tggcgtgtta aagaaaccga tcgcctgttt
     5041 gcgatggcaa cagaactgcg taaagtcggt gcggaagtag aagaggggca cgattacatt
     5101 cgtatcactc caccggaaaa actgaacttt gccgagatcg cgacatacaa tgatcaccgg
     5161 atggcgatgt gtttctcgct ggtggcgttg tcagatacac cagtgacgat tcttgatccc
     5221 aaatgtacgg ccaaaacatt tccggattat ttcgagcagt tggcgcggat tagccaggca
     5281 gcctgaatga acaacgggca ataaatagcc aaatctttct ttatcaaaac gtcggcacat
     5341 tgtcggcgtt ttttttcgga ccttgtgagt cattttgatt aatggtagcg tcgcttgtca
     5401 atgtaagttg ttgatacata atatttatat atgattaatc aacggatgat tcacatgaag
     5461 aatactaaat tactgctggc gattgcgacc tctgcagcat tactgacagg gtgtcaaaat
     5521 acccacggta ttgataccaa tatggctatc agctccggtt taaatgccta taaagcagca
     5581 acattaagcg atgccgatgc aaaagcgatt gccaatcagg gctgtgccga aatggacagc
     5641 ggcaatcaag tcgcaagtaa atccagcaag tacggtaaac gtctggcaaa aatcgccaaa
     5701 gcattgggta acaatattaa tggcacgccg gtcaactata aggtttatat gaccagcgac
     5761 gtcaacgcat gggcgatggc gaacggctgt gtccgtgtct acagtggcct gatggacatg
     5821 atgaatgaca acgaaattga aggtgttctg ggccatgaac tgggccacgt cgcgttgggt
     5881 cactcgctgg ctgaaatgaa agcttcttat gcgatcgttg ccgcacgcga tgccatttca
     5941 gctaccagcg gagtggcttc ccagctttcc cgctcacaat tgggtgatat cgcagaaggc
     6001 gctattaatg ctaaatactc ccgtgataaa gagtccgaag cagatgattt ctcctttgat
     6061 ctgttgaaga aacgtggcat cagcacccag gggctggttg gcagctttga aaaactggct
     6121 agtctggatg gcggtcgcac ccagtccatg tttgactctc acccaccatc aacagagcgt
     6181 gcgcaacaca tccgtgatcg tatcgcctct ggtaagtaaa tcattgtcat ctttcgggct
     6241 ggtcttctgc cagcccgcta taattgcgca ataaatcccc atctgaatac agacaaaact
     6301 ggtttttgca cacaacgtta acgatttgtg gcgtcggcgc gtataatgcg cgcggttatg
     6361 ttaacggtac gcctgtttta aggagataaa gatgacggca attgccccgg ttattaccat
     6421 tgatggccca agcggtgcag ggaaaggcac cttgtgtaag gctatggcgg aagcattgca
     6481 atggcatctg ctggattcgg gtgcaattta tcgcgtactg gcattggcgg cattacatca
     6541 ccatgttgat gttgcgtcag aagatgcgct ggtaccgctg gcatcccatc tggatgtacg
     6601 ttttgtgtca accaatggca atctggaagt gatcctcgaa ggggaagatg tcagcggcga
     6661 aattcgtact caggaagtgg cgaatgcagc ttcacaagtc gcggcattcc cacgcgttcg
     6721 tgaagcatta ttgcgtcgcc aacgcgcgtt tcgcgaatta ccaggcctga ttgccgatgg
     6781 ccgcgacatg ggaacggtgg tattccctga tgcgccagtg aaaattttcc ttgacgcctc
     6841 ctcggaagaa cgtgcgcatc gccgcatgct acagttgcag gagaagggct ttagtgttaa
     6901 ctttgagcgc cttttggccg agatcaaaga acgcgacgac cgcgatcgta accgagcggt
     6961 agcgccactg gttccggcag ccgatgcttt agtgttggat tccaccacct taagcattga
     7021 gcaagtgatt gaaaaagcgc tacaatacgc gcgccagaaa ttggctctcg cataagcgac
     7081 cgaatttgca gtacccccgt tgcaatggaa tgacagcggg tatgttaaac aaccccatcc
     7141 ggcatggagc caggtggacg ttaaatataa acctgaagat taaacatgac tgaatctttt
     7201 gctcaactct ttgaagagtc cttaaaagaa atcgaaaccc gcccgggttc tatcgttcgt
     7261 ggcgttgttg ttgctatcga caaagacgta gtactggttg acgctggtct gaaatctgag
     7321 tccgccatcc cggctgagca gttcaaaaac gcccagggcg agctggaaat ccaggtaggt
     7381 gacgaagttg acgttgctct ggacgcagta gaagacggct tcggtgaaac tctgctgtcc
     7441 cgtgagaaag ctaaacgtca cgaagcctgg atcacgctgg aaaaagctta cgaagatgct
     7501 gaaactgtta ccggtgttat caacggcaaa gttaagggcg gcttcactgt tgagctgaac
     7561 ggtattcgtg cgttcctgcc aggttctctg gtagacgttc gtccggtgcg tgacactctg
     7621 cacctggaag gcaaagagct tgaatttaaa gtaatcaagc tggatcagaa gcgcaacaac
     7681 gttgttgttt ctcgtcgtgc cgttatcgaa tccgaaaaca gcgcagagcg cgatcagctg
     7741 ctggaaaacc tgcaggaagg catggaagtt aaaggtatcg ttaagaacct cactgactac
     7801 ggtgcattcg ttgatctggg cggcgttgac ggcctgctgc acatcactga catggcctgg
     7861 aaacgcgtta agcatccgag cgaaatcgtc aacgtgggcg acgaaatcac tgttaaagtg
     7921 ctgaagttcg accgcgaacg tacccgtgta tccctgggcc tgaaacagct gggcgaagat
     7981 ccgtgggtag ctatcgctaa acgttatccg gaaggtacca aactgactgg tcgcgtgacc
     8041 aacctgaccg actacggctg cttcgttgaa atcgaagaag gcgttgaagg cctggtacac
     8101 gtttccgaaa tggattggac caacaaaaac atccacccgt ccaaagttgt taacgttggc
     8161 gatgtagtgg aagttatggt tctggatatc gacgaagaac gtcgtcgtat ctccctgggt
     8221 ctgaaacagt gcaaagctaa cccgtggcag cagttcgcgg aaacccacaa caagggcgac
     8281 cgtgttgaag gtaaaatcaa gtctatcact gacttcggta tcttcatcgg cctggacggc
     8341 ggcatcgacg gcctggttca cctgtctgac atctcctgga acgttgcagg cgaagaagca
     8401 gttcgtgaat acaaaaaagg cgacgaaatc gctgcagttg ttctgcaggt tgacgcagaa
     8461 cgtgaacgta tctccctggg cgttaaacag ctcgcagaag atccgttcaa caactgggtt
     8521 gctctgaaca agaaaggcgc tatcgtaacc ggtaaagtaa ctgcagttga cgctaaaggc
     8581 gcaaccgtag aactggctga cggcgttgaa ggttacctgc gtgcttctga agcatcccgt
     8641 gaccgcgttg aagacgctac cctggttctg agcgttggcg acgaagttga agctaaattc
     8701 accggcgttg atcgtaaaaa ccgcgcaatc agcctgtctg ttcgtgcgaa agacgaagct
     8761 gacgagaaag atgcaatcgc aactgttaac aaacaggaag atgcaaactt ctccaacaac
     8821 gcaatggctg aagctttcaa agcagctaaa ggcgagtaat tctctgactc ttcgggattt
     8881 ttattccgaa gtttgttgag tttacttgac agattgcagg tttcgtcctg taatcaagca
     8941 ctaagggcgg ctacggccgc ccttaatcaa tgcagcaaca gcagccgctt aatttgcctt
     9001 taaggaaccg gaggaatcat gaccaagtca gaattgatag aaagacttgc cacccagcaa
     9061 tcgcacattc ccgccaagac ggttgaagat gcagtaaaag agatgctgga gcatatggcc
     9121 tcgactcttg cgcagggcga gcgtattgaa atccgcggtt tcggcagttt ctctttgcac
     9181 taccgcgcac cacgtaccgg acgtaatccg aagactggcg ataaagtaga actggaagga
     9241 aaatacgttc ctcactttaa accaggtaaa gaactgcgcg atcgcgccaa tatttacggt
     9301 taagttttta ctcaaacttg aacgcgagaa aagcacctgt cgggtgcttt tttcatttct
     9361 ctaatcagga actggaagct gcctcgcaga gttttgagca gttttcaccc tctcgttaaa
     9421 ttcttctgaa cgtgcctcgg ggaacgcaaa attcccacca gacaaccgct caacaaagtt
     9481 gcacactttc cataaacagg gaggggtgca tgaaaataac gacagtcggc gtatgcataa
     9541 tttgcggaat ttttccgttg ctgattttgc cccaattgcc tgggacatta acccttgcgt
     9601 ttctgactct cttcgcctgc gtactggcat ttatccctgt taaaaccgtc cgttatatcg
     9661 cgctgacgtt gttgtttttc gtttggggca tattagcagc aaagcaaatt ttgtgggcag
     9721 gagaaacctt aactggcgcg acgcaggatg caattgttga gatcactgct actgacggta
     9781 tgaccactca ttacggtcaa attactcatc tacaaggtcg acgtatattc cctgcgccaa
     9841 gccttgtgct gtatggcgaa tatcttccgc aagcggtttg tgccggacaa ctgtggtcaa
     9901 tgaaactcaa agttcgtgcc gttcatggtc aacttaatga tggcggcttt gatagccagc
     9961 gttatgccat tgcccagcat cagccgctca ccggccgctt tctgcaggca agtgttattg
    10021 aaccgaattg tagcctgcgt gcacagtatc tggcgtcact acaaacaacg ctgcaaccct
    10081 atccgtggaa tgcggttatt cttggtttag gtatggggga acggttatct gtccctaaag
    10141 aaatcaaaaa tatcatgcgt gatactggaa cggcgcattt aatggcgata tcgggattgc
    10201 acatcgcttt tgcggcgttg ctggctgccg gactcattcg cagtggacaa atttttctgc
    10261 ctgggcgctg gatccactgg caaatgccat taattggcgg aatctgctgt gctgcttttt
    10321 atgcctggct gacgggaatg caacctcctg cattgcgtac cgttgtggcg cttgctacgt
    10381 ggggaatgct taagttaagt gggcgacagt ggagtggctg ggatgtatgg atatgttgtc
    10441 tggcggcaat tttgctgatg gatcctgttg ccattctctc gcaaagttta tggctctctg
    10501 ccgctgcggt cgcggcattg atattttggt atcagtggtt tccctgtcct gagtggcaac
    10561 tgccgccggt attgcgtgca gttgtttccc tcatccatct gcaactggga atcacacttc
    10621 tgcttatgcc cgtgcaaatc gtcatatttc attgcattag tctgacctcg tttattgcaa
    10681 atctattagc aattcccctg gtgacattta tcacggttcc gttgatcctc gccgcgatgg
    10741 ttgtgcattt aagcgggccg ttaatcctgg agcaagggtt atggtttctt gccgaccggt
    10801 ctttggcttt acttttctgg gggttaaaga gtttgccgga agggtggatc aacattgctg
    10861 aatgttggca atggctatca ttttccccat ggttcttact ggtggtatgg cgattaaacg
    10921 cctggcgaac gttgccagca atgtgtgtgg ctggaggctt gctgatgtgc tggccgctgt
    10981 ggcaaaaacc tcgacctgac gagtggcagc tgtacatgct tgatgtcggg caagggctgg
    11041 caatggtgat agccagaaac ggcaaagcga ttctctatga cacaggactg gcctggcccg
    11101 aaggggatag tgggcaacaa ctgattatcc cctggctcca ctggcataat cttgaaccgg
    11161 aaggcgttat tctgagtcat gaacatctgg atcaccgggg agggctggac tcaatattgc
    11221 acacatggcc gatgttatgg atcagaagtc cgttaaactg ggaacatcat cagccctgtg
    11281 tgcgtggcga agcgtggcaa tggcaaggat tgcgtttcag cgtgcactgg cctttacaag
    11341 ctagcaacga taaaggaaat aaccattcct gtgtggttaa ggttgatgac gggacgaata
    11401 gcattcttct aaccggtgat attgaagtcc ccgctgaaca aaagatgcta agccgttact
    11461 ggcagcaagt gcagacaaca ttgcttcagg tacctcacca tggcagtaat acctcatcat
    11521 cgttgccatt aattcagcga gtgaatggaa aagtggcact cgcatcggca tcgcgctata
    11581 acgcatggcg attgccctct aataaagtta agcatcgcta tcaacagcaa ggatatcaat
    11641 ggcttgatac tccacatcag ggtcaagtga cggtcaattt ttcagcgcaa ggctggcgga
    11701 ttagcagcct cagagagcaa attttacctc gttggtatca tcagtggttt ggcgtgccag
    11761 tggataacgg gtagaatatg cggctatttc aacaaatgct ggttttttga atgcataacg
    11821 acaaagatct ctctacgtgg cagacgttcc gccgactgtg gccaaccatt gcgcctttta
    11881 aagcgggtct gatcgtggcg ggcgtagcgt taatcctcaa cgcagccagc gataccttca
    11941 tgttatcgct ccttaagcca cttcttgatg atggctttgg taaaacagat cgctccgtgc
    12001 tggtgtggat gccgctggtg gtgatcgggc tgatgatttt acgtggtatc accagctatg
    12061 tctccagcta ctgtatctcc tgggtatcag gaaaggtggt aatgaccatg cgtcgccgcc
    12121 tgtttggtca catgatggga atgccggttt cattttttga caaacagtca acggggacgc
    12181 tgttgtcacg tatcacctac gattccgaac aggttgcttc ttcttcttcc ggcgcactga
    12241 taacggttgt gcgtgaaggt gcgtcaatca tcggcctgtt catcatgatg ttctattaca
    12301 gttggcaact gtcgatcatt ttgattgtgc tggcaccgat tgtttcgatt gcgattcgcg
    12361 tagtatcgaa gcgttttcgc aacatcagta aaaacatgca gaacaccatg gggcaggtga
    12421 ccaccagcgc cgaacaaatg ctgaagggcc acaaagaagt attgattttc ggtggtcagg
    12481 aagtggaaac gaaacgcttt gataaagtca gcaaccgaat gcgtcttcag gggatgaaaa
    12541 tggtttcagc ctcttccatc tctgatccga tcattcagct gatcgcctct ttggcgctgg
    12601 cgtttgttct gtatgcggcg agcttcccaa gtgtcatgga tagcctgact gccggtacga
    12661 ttaccgttgt tttctcttca atgattgcac tgatgcgtcc gctgaaatcg ctgaccaacg
    12721 ttaacgccca gttccagcgc ggtatggcgg cttgtcagac gctgtttacc attctggaca
    12781 gtgagcagga gaaagatgaa ggtaagcgcg tgatcgagcg tgcgactggc gacgtggaat
    12841 tccgcaatgt cacctttact tatccgggac gtgacgtacc tgcattgcgt aacatcaacc
    12901 tgaaaattcc ggcagggaag acggttgctc tggttggacg ctctggttcg ggtaaatcaa
    12961 ccatcgccag cctgatcacg cgtttttacg atattgatga aggcgaaatc ctgatggatg
    13021 gtcacgatct gcgcgagtat accctggcgt cgttacgtaa ccaggttgct ctggtgtcgc
    13081 agaatgtcca tctgtttaac gatacggttg ctaacaacat tgcttacgca cggactgaac
    13141 agtacagccg tgagcaaatt gaagaagcgg cgcgtatggc ctacgccatg gacttcatca
    13201 ataagatgga taacggtctc gatacagtga ttggtgaaaa cggcgtgctg ctctctggcg
    13261 gtcagcgtca gcgtattgct atcgctcgag ccttgttgcg tgatagcccg attctgattc
    13321 tggacgaagc tacctcggct ctggataccg aatccgaacg tgcgattcag gcggcactgg
    13381 atgagttgca gaaaaaccgt acctctctgg tgattgccca ccgcttgtct accattgaaa
    13441 aggcagacga aatcgtggtc gtcgaggatg gtgtcattgt ggaacgcggt acgcataacg
    13501 atttgcttga gcaccgcggc gtttacgcgc aacttcacaa aatgcagttt ggccaatgat
    13561 cgaaaaaatc tggtctggtg aatccccttt gtggcggcta ttgctgccac tctcctggtt
    13621 gtatggcctg gtgagtggcg cgatccgtct ttgctataaa ctaaaactga agcgcgcctg
    13681 gcgtgccccc gtaccggttg tcgtggttgg taatctcacc gcaggcggca acggaaaaac
    13741 cccggtcgtt gtctggctgg tggaacagtt gcaacagcgc ggtattcgcg tgggggtcgt
    13801 atcgcgggga tatggtggta aggctgaatc ttatccgctg ttattgtcgg cagataccac
    13861 aacagcacag gcgggtgatg aacctgtgtt gatttatcaa cgcactgatg cgcctgttgc
    13921 ggtttctccc gttcgttctg atgcggtaaa agccattctg gcgcaacacc ctgatgtgca
    13981 gatcatcgta accgacgacg gtttacagca ttaccgtctg gcgcgtgatg tggaaattgt
    14041 cgttattgat ggtgtgcgtc gctttggcaa tggctggtgg ttgccggcgg ggccaatgcg
    14101 tgagcgagcg gggcgcttaa agtcggttga tgcggtaatc gtcaacggcg gtgtccctcg
    14161 cagcggtgaa atccccatgc atctgctgcc gggtcaggcg gtgaatttac gtaccggtac
    14221 gcgttgtgac gttgctcagc ttgaacatgt agtggcgatg gcggggattg ggcatccgcc
    14281 gcgctttttt gccacgctga agatgtgtgg cgtacaaccg gaaaaatgtg taccgctggc
    14341 cgatcatcag tctttgaacc atgcggatgt cagtgcgttg gtaagcgccg ggcaaacgct
    14401 ggtaatgact gaaaaagatg cggtgaaatg ccgggccttt gcagaagaaa attggtggta
    14461 tttgcctgta gacgcacagc tttcaggtga tgaaccagcg aaactgctta cgcaactaac
    14521 ctcgctggct tctggcaact agttacgccg cggcagcgtt cgattgatgg agtcatgaat
    14581 gtcgctgccg cacctctccc ttgctgatgc gcgtaatctt caccttgccg cacaaggcct
    14641 gttaaacaaa ccccgccgtc gagcgtcgtt ggaggatatt ccggcaacga tctcccgcat
    14701 gtccttgctg caaatcgata ccatcaatat tgttgcccgt agtccatatc tggtgctttt
    14761 cagtcgtctg ggaaattatc ctgcccagtg gctggatgag tctctggcgc gtggcgaatt
    14821 aatggaatac tgggcgcatg aagcctgctt tatgccgcgt agcgactttc gtcttattcg
    14881 ccaccgcatg ctggcacctg aaaaaatggg ctggaaatac aaagacgcct ggatgcagga
    14941 acatgaggcg gaaattgcac agttaattca gcatattcat gataaggggc cggtacgttc
    15001 agccgatttt gagcatcctc gtaaaggtgc aagcggctgg tgggaatgga agccgcataa
    15061 acggcatctg gaaggtttat ttactgccgg aaaggtgatg gtgattgaac ggcgcaactt
    15121 ccagcgcgtt tatgatttaa cccaccgtgt catgcctgac tgggatgatg agcgcgatct
    15181 cgtttcgcaa acagaagcag aaatcatcat gctggataac agtgcgcgta gcctgggaat
    15241 attccgcgaa cagtggctgg cagattacta tcggctgaaa cgtccggcac tggcggcgtg
    15301 gcgcgaagcg agggctgaac agcagcaaat cattgctgtg catgttgaaa aattgggcaa
    15361 tctttggctg catgctgatt tgctgccgct actcgagcgt gcgctggccg gaaagctcac
    15421 tgcaacgcac agcgcggtac tttcgccttt tgatcctgtt gtctgggatc gcaaacgcgc
    15481 agagcagctt tttgatttta gctaccggct ggagtgctat accccagcgc cgaaacgcca
    15541 gtatggctat tttgttctgc cattattaca tcgtgggcaa ttagttgggc gaatggatgc
    15601 caaaatgcat cgccagacag gcatccttga agttatctct ctgtggttac aggaaggcat
    15661 taaaccaacg acaatgctgc aaaaagggtt acgtcaggcg attactgatt tcgcgagctg
    15721 gcagcaggca acgcgggtga cattaggacg ctgcccgcaa ggtctcttta ctgattgccg
    15781 cactggctgg gaaatagacc ccgtcgcata aatgattatg ataagattta tgcattcata
    15841 acccgatcca tctggaggaa ctatggatca tcgtttgctt gaaatcattg cctgcccggt
    15901 ttgcaacgga aaactttggt ataaccagga aaaacaagag cttatttgca aactggataa
    15961 cctggctttc ccgctccgcg atggcattcc agtgttgctg gaaacggaag cccgcgtgct
    16021 gactgctgat gagagtaaat catgagtttt gtggtcatta ttcccgcgcg ctacgcgtcc
    16081 acgcgtctgc ccggtaaacc attggttgat attaacggca aacccatgat tgttcatgtt
    16141 cttgaacgcg cgcgtgaatc aggtgccgag cgcatcatcg tggcaaccga tcatgaggat
    16201 gttgcccgcg ccgttgaagc cgctggcggt gaagtatgta tgacgcgcgc cgatcatcag
    16261 tcaggaacag aacgtctggc ggaagttgtc gaaaaatgcg cattcagcga cgacacggtg
    16321 atcgttaatg tgcagggtga tgaaccgatg atccctgcga caatcattcg tcaggttgct
    16381 gataacctcg ctcagcgtca ggtgggtatg gcgactctgg cggtgccaat ccgcaatgcg
    16441 gaagaagcgt ttaacccgaa tgcggtgaaa gtggttctcg acgctgaagg gtatgcactg
    16501 tacttctcac gcgccaccat tccttgggat cgtgatcgtt ttgcagaagg ccttgaaacc
    16561 gttggcgata acttcctgcg tcatcttggt atttatggct accgtgcagg ctttatccgt
    16621 cgttacgtca actggcaggc aagtccgtta gaacacatcg aaatgttaga gcagcttcgt
    16681 gttctgtggt acggcgaaaa aatccatgtt gctgttgcgc atgaagttcc tggcacaggt
    16741 gtggataccc ctgaagatct cgagcgcgtt cgcgctgaaa tgcgctaatt tcacttcacg
    16801 acacttcagc caattttggg aggagtgtcg taccgttacg attttcctca atttttcttt
    16861 tcaacaattg atctcattca ggtgacatct tttatattgg cgctcattat gaaagcagta
    16921 gcttttatga gggtaatctg aatggaacag ctgcgtgccg aattaagcca tttactgggc
    16981 gaaaaactca gtcgtattga gtgcgtcaat gaaaaagcgg atacggcgtt gtgggctttg
    17041 tatgacagcc agggaaaccc aatgccgtta atggcaagaa gctttagtac gcccggaaaa
    17101 gcccgacaac tggcatggaa aaccaccatg ctggcaagaa gtgggactgt ccgtatgccg
    17161 actatttatg gtgtgatgac gcatgaagaa caccccggcc ccgatgtcct gctactggag
    17221 cggatgcgtg gtgtttcggt ggaggcacca gcccgaacac cagaacgctg ggaacaactc
    17281 aaagaccaaa tcgttgaagc cttactggcc tggcaccgtc aggacagtcg cggttgcgtc
    17341 ggcgcggtcg acaatactca ggaaaatttc tggccctcat ggtaccggca acatgttgaa
    17401 gtgctatgga ccacgctcaa tcagttcaat aacaccggtc tgacgatgca ggataagcgg
    17461 atcctgtttc gcactcgcga atgtctcccg gcattatttg aaggctttaa cgacaattgt
    17521 gtgttgattc acggtaactt ctgtttacgc agcatgctga aagattcgcg cagcgatcag
    17581 ttactggcga tggtcgggcc gggactaatg ctttgggcac cgcgagaata cgaactgttc
    17641 cgactaatgg ataattctct ggcggaagat ttgctctgga gttacctgca acgcgcgcca
    17701 gtggcggagt cgttcatctg gcggcgttgg ttgtatgtgt tatgggatga agttgcgcaa
    17761 ctggttaata ccggacgatt tagtcggcgc aacttcgatc tggcatcaaa atcactcttg
    17821 ccgtggctcg cctgacggac ctttcagcca ctgccagatg cggccaagcg tttcatagcc
    17881 aacacgatcg ctatgcatca gccagaccgg agaagggatt gcccgttccc acggattaag
    17941 cggcgagtcg atggccaact gattcgccgg ggctggtagc ggatttaacc cttcctgctg
    18001 aaaaaagatc atcgcccgcg gcagatgtga tgcggaggtc accagcagga aaggggcgtc
    18061 accaatcgcc tgtttcactg ccgcagcttc ttcttcggta tcttttggca aatccagggt
    18121 gataatttgc tcgcgcggca cgcccagcga ttgcgcaact ctggcaccta cttccgctgt
    18181 actcaccgta ttggtttttg ctacgcctcc cgtgaagatc agttttgatc ccggattttc
    18241 gcgccataag cgaatacctt cattcaggcg tggcagactg ttattgatta aattagagct
    18301 cggtgcccac tgtgggttcc aggtgtaacc acctccgagc accacgatat agtccacttt
    18361 ttgcgaatta ttccatgtcg gataggtgct ttcgatggga cgcagcaggc ggtcggctac
    18421 cggttgcaaa ctcaataaca agagtgccag ccacccaatg ctaataaaaa tcttcccggt
    18481 tttttgaaaa cggctaaacc acagcagcgc caggccagcg ccaataatta acaacatcag
    18541 cggaagtggc agcaacatat tgccaatcac ttttttcagt gtaaaaagca tcctttctgg
    18601 ttcctttttt aaccatatag caagggatcg ccagcgatat tacaccagac gggttcattc
    18661 tcgccgtggc tgtgacaaaa tagcgtcttt atcagaaaac gtcttagcca accggtggag
    18721 aaagcaatgc aggatcgcaa tttcgatgat attgcggaaa agttttcccg taacatttac
    18781 ggcaccacca aagggcagct tcgacaggct attctgtggc aggatctcga tcacgtgctg
    18841 gcggaaatgg gcccgcaaaa actgcgtgtg ctggatgctg gcggtggaga agggcagacc
    18901 gcaatcaaaa tggccgagcg tgggcatcag gtcattttat gcgatctttc tgcgcagatg
    18961 atcgaccgcg caaaacaggc ggcagaagca aaaggtgtga gcgacaacat gcaatttata
    19021 cattgcgccg ctcaggatgt tgcttcgcat ttggaaacgc ccgttgatct gatattgttc
    19081 catgcggtgc tcgagtgggt ggccgatccc cgcagtgtat tgcagaccct gtggtcagtg
    19141 ttgcgtccag gcggagtgtt gtcgttaatg ttctacaatg cgcatggttt gttgatgcat
    19201 aacatggtcg ccgggaattt tgattacgtg caggcgggaa tgccgaaaaa gaaaaaacgg
    19261 acgctttcgc cagattatcc acgcgacccg gcgcaggttt atctgtggct ggaagaagct
    19321 ggttggcaaa ttatgggtaa gacaggcgtt cgcgtgtttc atgattatct gcgcgagaag
    19381 caccagcagc gcgactgcta tgaagcatta cttgaattag aaacgcgtta ttgccgtcag
    19441 gaaccgtata ttaccctggg gcgttatatt catgtcaccg cgcgcaaacc gcagagcaag
    19501 gataaagtat gagtgaattt tcccagacag tccccgaact ggttgcctgg gccagaaaaa
    19561 atgacttctc catctcgctg ccggtagacc gactctcttt tctgctggcg gttgccacgc
    19621 tgaacggcga gcgtctggat ggtgagatga gtgaaggcga gctggtggat gcattccgcc
    19681 atgtgagtga tgcgtttgag caaaccagcg aaaccatcgg cgtgcgcgcc aacaacgcga
    19741 tcaacgacat ggtgcgtcaa cgtctgctga accgctttac cagcgagcag gcggaaggga
    19801 acgcaattta ccgtctgacg ccgctcggca tcggcatcac tgactattac attcgtcagc
    19861 gcgagttttc tacgctgcgc ctttctatgc agttgtcgat tgtggcgggt gagctcaaac
    19921 gcgcagcaga tgccgccgaa gagggcggtg atgaatttca ctggcaccgt aatgtttatg
    19981 ctccactgaa atattcggta gcagaaattt tcgacagtat cgacctgacg caacgtctga
    20041 tggatgaaca gcagcagcag gtgaaggatg atatcgccca gttgctgaac aaagactggc
    20101 gggcggcgat ttccagctgt gaattgttgc tttcggaaac ttccggaacg ctgcgtgaat
    20161 tgcaggatac gctggaagcg gcaggcgaca aattgcaggc taatctgttg cgcattcagg
    20221 atgcgacgat gacccatgac gatctgcatt tcgtcgatcg tctggtgttc gatctgcaga
    20281 gcaaactcga tcgtattatc agttggggcc agcaatccat cgacttgtgg attggctacg
    20341 accgccacgt acacaaattt attcgtaccg cgatcgatat ggataaaaac cgcgtctttg
    20401 ctcagcggtt acgtcagtcg gtacaaacct attttgatga gccgtgggcg ctaacttatg
    20461 ccaatgccga tcgtctgctg gatatgcgtg acgaagagat ggcactgcgc gatgaagaag
    20521 tgactgggga acttcctgag gatctggaat acgaagagtt taacgagatc cgcgaacagc
    20581 tggcggcgat catcgaagaa caacttgccg tgtacaaaac cagacaagtg ccgctggatc
    20641 ttggtctggt ggtacgcgaa tatctgtcac agtatccgcg tgcacgtcac tttgacgttg
    20701 cgcgtattgt tattgatcag gcggtacgtc ttggcgtagc gcaagcagat ttcaccggac
    20761 tgccagcgaa atggcagccg attaatgatt acggagccaa ggtacaggcg catgtcatcg
    20821 acaaatattg aacaagtgat gccggttaag ctggcgcagg cgctggcgaa tccgttattt
    20881 ccggcgctgg acagcgcctt acgttcaggc cgccatattg gcctcgacga actggataat
    20941 catgcattcc tgatggattt tcaggaatat ctggaagagt tttacgcgcg ttataacgtt
    21001 gagcttattc gcgcaccaga agggttcttc tatttacgcc cacgttccac cacgctgatc
    21061 cctcgttccg tcttgtcgga actggatatg atggtcggga aaatcctctg ttatctctat
    21121 ctcagcccgg aacggctggc gaatgagggg attttcaccc agcaggaact gtacgacgaa
    21181 ctgctcaccc tggccgatga agcaaaactg ctgaaactgg tgaacaaccg ttcaaccggt
    21241 tcagacgttg accgtcagaa gttgcaggag aaagtacgtt cttcgctcaa ccgtctgcgt
    21301 cgtttaggca tggtgtggtt tatgggccac gacagcagca agtttcgcat taccgaatcg
    21361 gtgttccgct tcggagccga tgtgcgtgct ggcgacgatc cccgtgaagc acagcgtcgc
    21421 ctgattcgtg atggcgaagc aatgccgatt gaaaatcatc tgcaactcaa cgatgaaacc
    21481 gaagagaatc agccagatag cggagaggaa gaataatgat tgaacgcggt aaatttcgct
    21541 cactgacgct gattaactgg aacggctttt ttgcccgaac ttttgacctt gacgagctgg
    21601 tcacgacgct ttctggcggt aacggggcgg gtaaatccac caccatggcg gcgttcgtta
    21661 cggcgctgat ccccgacctg accctgctgc atttccgtaa cactacggaa gccggggcca
    21721 ccagcggttc gcgcgataaa ggtctgcacg gtaagctgaa agcgggtgtc tgttattcga
    21781 tgctcgacac cattaactcg cgccaccagc gcgtggtggt cggtgtgcgt ctgcaacagg
    21841 ttgccggacg cgatcgtaaa gtggatatca aaccatttgc cattcaggga ctgccgatgt
    21901 cggtgcagcc gacacagctg gtgaccgaaa ccctgaacga acgccaggcg cgcgtgctgc
    21961 cgcttaacga gctgaaagac aagctcgagg cgatggaagg cgtgcagttt aaacagttca
    22021 actccattac cgattaccac tcgctgatgt tcgatcttgg catcatcgcg cgtcgtctgc
    22081 gctccgcatc tgaccgtagc aaattctatc gtctgatcga agcttcgctg tatggcggga
    22141 tctccagtgc cattacccgt tctctgcgcg actacctgtt gccggaaaac agcggcgtgc
    22201 gtaaagcgtt ccaggacatg gaagcggcgc tgcgtgaaaa ccgcatgacg ctggaagcga
    22261 ttcgtgtcac ccagtcggac cgtgacctgt ttaagcatct gatcagcgaa gctaccaact
    22321 acgtggcggc ggactacatg cgtcacgcca acgagcgccg tgtccatctc gacaaagccc
    22381 tggagtttcg tcgcgagtta catacttcgc gtcagcaact ggcggctgag cagtacaaac
    22441 acgtcgatat ggcgcgtgag ctggcagagc acaacggtgc cgaaggcgat ctggaagcgg
    22501 attatcaggc ggccagcgat cacctgaacc tggtgcaaac cgcactgcgt cagcaggaga
    22561 agatcgaacg ctacgaagcg gatctcgatg agctgcagat ccgtctggaa gagcaaaacg
    22621 aagtggtggc agaagccatc gaacgccagg aagagaacga ggctcgcgcg gaagctgccg
    22681 aactggaagt ggacgagctg aaaagccagc ttgctgacta ccagcaggcg ctggacgtcc
    22741 agcaaacgcg cgcgatccag tataaccagg cgattgctgc gcttaatcgc gccaaagaac
    22801 tgtgccatct gccggactta accgccgaca gcgccgccga atggctggaa accttccagg
    22861 cgaaagagct ggaagcgact gaaaaaatgc tctctcttga gcagaaaatg agcatggcgc
    22921 aaaccgcgca cagccagttt gagcaggctt atcagctggt ggtggcaatc aacggcccgc
    22981 tggcgcgtaa cgaggcgtgg gatgtcgctc gcgaactatt gcgcgaaggg gtcgatcagc
    23041 gtcacctggc agagcaggtt cagccgttgc ggatgcgctt aagcgaactg gaacagcgtc
    23101 tgcgcgaaca gcaagaagct gagcgtctgc tggcagattt ctgcaaacgt cagggcaaga
    23161 attttgacat tgacgagctg gaagctctgc atcaggagct ggaagcgcgc attgcctctc
    23221 tttccgatag cgtgtctaac gcccgtgaag agcgcatggc actgcgccag gaacaggaac
    23281 agctgcagtc tcgcattcag agtttgatgc agcgtgcgcc ggtttggctg gcagcgcaaa
    23341 acagtctcaa ccagttgagc gaacagtgcg gcgaagagtt tacctccagc caggacgtca
    23401 ccgaatatct gcaacagttg ctggagcgtg agcgagaggc gattgttgaa cgcgatgaag
    23461 tgggcgcgcg caaaaacgcc gtcgatgaag agatcgaacg tttaagccag cctggcggct
    23521 ctgaagatca gcgtctgaac gcgctggcgg agcgttttgg tggtgtgctg ctgtcagaaa
    23581 tttatgacga cgttagcctg gaagatgcgc cgtacttctc agcgctgtat ggcccgtcac
    23641 gccacgccat cgtggtgcca gatctgtcac aggtaactga acacctggaa ggcttgaccg
    23701 attgcccgga agatctctat ctgatcgaag gagatccgca gtcattcgat gacagcgtgt
    23761 tcagcgttga tgagctggaa aaagcggtag tggtgaaaat cgccgatcgt cagtggcgtt
    23821 attcacgttt cccggaagtg ccgctgtttg gtcgtgctgc gcgtgaaagc cgtattgaaa
    23881 gcctccatgc cgagcgtgaa gtgctttccg aacgcttcgc cacgctctcc tttgatgtac
    23941 agaaaactca gcgtctgcat caggcgttca gccgctttat cggcagtcat ctggcggttg
    24001 cgtttgagtc tgacccggaa gcagaaatcc gtcaactgaa cagccgtcgc gtcgaactgg
    24061 agcgggcgtt gagtaatcat gaaaatgata accagcagca gcgtattcag tttgagcagg
    24121 cgaaagaagg cgttacggcg ctgaaccgca ttctgccgcg tctcaacctg ttggctgatg
    24181 acagcctggc ggatcgcgtc gatgaaatcc gcgaacgtct ggatgaagcc caggaagccg
    24241 cgcgttttgt tcagcagttt ggcaatcaac tggcgaaact ggaaccgatc gtttcggtat
    24301 tgcagagcga cccggaacag ttcgaacagc taaaagaaga ttacgcgtac tctcagcaga
    24361 tgcagcgcga tgcccgtcag caggcgtttg ccctgacgga agtggtgcag cgtcgtgcgc
    24421 actttagcta ttctgactcg gcagaaatgc tgagcggtaa tagcgatctc aacgaaaagc
    24481 tgcgtgaacg tctggaacag gcggaagcgg agcgtactcg cgctcgtgaa gcgttgcgtg
    24541 gtcacgcagc gcagttgagt cagtacaacc aggtgctggc ttcgctgaaa agttcttacg
    24601 acaccaaaaa agagctactc aacgatctgc aacgtgaatt gcaggatatc ggcgtgcgtg
    24661 ctgatagcgg ggcagaagag cgggcgcgta ttcgccgtga cgagctgcat gcgcaactga
    24721 gcaataaccg ttcacgccgc aatcaactgg aaaaagcgct taccttctgc gaagcggaga
    24781 tggacaacct gacccgcaaa ctgcgcaagc tggagcggga ttactttgag atgcgcgagc
    24841 aggtagtgac cgccaaagcg ggctggtgtg cagtgatgcg catggtgaaa gataacggcg
    24901 ttgaacggcg cttacaccgt cgcgagctgg cttatctctc cgctgatgat ttgcgctcta
    24961 tgtcggataa ggcattgggt gcgctgcgtc tggcggtggc ggataacgaa catctgcgcg
    25021 acgtgctgcg catgtcggaa gatccaaaac gtccggaacg taaaattcag ttcttcgtgg
    25081 cggtttatca gcatctgcgt gaacgtattc gtcaggatat tattcgtacc gatgatccgg
    25141 tggaagccat cgagcagatg gaaatcgaac ttagccgact gaccgaggag ttaacctccc
    25201 gcgaacagaa actggcgatc agttcccgca gcgtggcgaa catcattcgc aaaaccattc
    25261 agcgcgagca gaaccgtatc cgtatgctca accaggggtt gcagaacgta tcgtttggtc
    25321 aggtgaacag cgtacgtctg aacgtgaacg tgcgtgaaac gcacgccatg ctgctggatg
    25381 tgctctctga acagcacgag cagcatcagg atctgtttaa cagcaaccgt ctgaccttct
    25441 cggaagcgct ggcgaaattg tatcagcgtc ttaatccgca gattgatatg gggcagcgca
    25501 cgccgcagac cattggcgaa gaactgctgg attaccgcaa ctatctggaa atggaagttg
    25561 aggttaatcg tggttccgat ggctggttgc gcgcagagtc tggtgcattg tcgaccggtg
    25621 aggcgattgg taccggtatg tcgattctgg tgatggtggt acaaagctgg gaagatgaat
    25681 ctcgccgcct gcgcggtaaa gatatctctc cttgccgcct gctgttcctc gatgaagcag
    25741 cgcgactgga tgctcgttct atcgccacgc tgtttgaatt gtgtgaacgt ttgcaaatgc
    25801 agctcatcat cgcagcgccg gaaaatatca gcccggagaa aggcaccacc tataaactgg
    25861 tgcgtaaagt cttccagaat accgaacacg ttcacgtcgt cggcctgcga ggatttgcgc
    25921 cgcaactccc tgaaacgctt ccaggaagtg acgaagcgcc ttctcaggcg agttaaaatt
    25981 aaggcggcag caaagccgcc ttttcttttt ccgaaaactc cgtttctgca ctaaaaaagt
    26041 ggcacattac ggcgcgcttt tctttaaact tctttacatt aggttatgta aaaacgtatc
    26101 ggcgtttata tactgaagat aagcctgatg agtaacaggc ttgctcgtca tactttcgtg
    26161 agtattggcg ttgtacaggc aagtcgtaaa ataacagcct ggctattcag agtatgataa
    26221 aaacaggggg caagggatgt tgcttaatat gatgtgtggt cgtcggctgt cggcaatcag
    26281 tttgtgcctg gccgtaacat tcgctccact gttcaatgcg caggccgatg agcctgaagt
    26341 aatccctggc gacagcccgg tggctgtcag tgaacagggc gaggcactgc cgcaggcgca
    26401 agccacggca atattggcgg ggatccagcc attgcctgaa ggtgcggcag aaaaagcccg
    26461 cactcaaatc gaatctcaat tacccgcagg ttacaagccg gtttatctta accagcttca
    26521 actgttgtat gccgcacgcg atatgcaacc catgtgggaa aaccgtgatg ctgttaaagc
    26581 cttccagcaa cagctggcag aggtggcgat tgccggtttc cagccgcagt ttaataaatg
    26641 ggtagagtta ctgaccgatc ctggtgttaa cgggatggca cgcgacgtag tgctctctga
    26701 tgcgatgatg ggctatctcc atttcattgc aaatattccg gtcaaaggca ctcgctggct
    26761 atatagcagt aaaccttatg cacttgcaac gccgccgctc tcggtgatta accaatggca
    26821 gctggcgctg gataaaggtc aattgcctac gtttgttgca ggactggcac cgcagcatcc
    26881 gcaatatgcg gcgatgcatg aatcgttact ggccttactc agtgacacca aaccgtggcc
    26941 ccaactgacc ggcaaagcaa cgttgcgccc agggcagtgg agtaacgacg taccggcgtt
    27001 gcgcgaaata ttgcaacgca caggcatgtt ggacgggggg ccgaaaatta ctctacctgg
    27061 cgatgacacg ccaactgacg cggtagtcag cccatccgct gttactgttg aaacagcaga
    27121 aactaagccg atggtcaagc aaatgacgtc tcgtagtaaa cctgcgcctg ccgttcgtgc
    27181 cgcctacgat aatgaactgg tggaagccgt taaacgtttt caggcatggc aaggattggg
    27241 ggcagatggt gctattggcc cggcaacacg tgactggtta aacgtaacgc ccgcccagcg
    27301 tgctggcgtg ttggctctca acatccagcg attgcgcttg ctgccaacag agctttctac
    27361 cgggatcatg gttaacattc cggcctattc gctggtctac tatcagaacg gcaatcaggt
    27421 gctggattcg cgagtcattg tcggtcgccc cgatcgcaaa acgccgatga tgagcagtgc
    27481 ccttaacaac gtagtggtaa acccgccgtg gaacgtacct ccaacactgg cacgcaaaga
    27541 tattctgcca aaagtgcgca acgatccggg atatctcgaa agccatggct atacggtgat
    27601 gcgcggctgg aacagcagag aagcgattga cccatggcag gttgactggt ctacaatcac
    27661 ggcctcgaat ttaccgttcc gcttccagca ggctccaggc ccacggaact cgctggggcg
    27721 ctataaattc aatatgccga gttcagaggc catttatttg catgacacgc cgaaccacaa
    27781 tctgttcaag cgtgatacac gcgcattgag ctcaggctgt gtacgagtga ataaagcttc
    27841 cgatctggcg aatatgctgt tgcaggatgc aggctggaat gacaaacgta tttctgatgc
    27901 gctgaagcag ggtgatacac gttacgtcaa tattcggcag tcgattccgg tgaatctcta
    27961 ctacctgacg gcctttgttg gtgcagatgg tcgtacccag tatcgtacag atatttacaa
    28021 ttatgatctg cctgcgcgat ccagctcgca aatcgtatcg aaagcggaac aattaatcag
    28081 gtaaatgaag tagttcgggg aaatcagttg tcgtaataat tagcatgatt gggggcgatt
    28141 ctctgcagcc cccgtcactg ctggggttga gtcatcttga cgtctgcttt acgggcggtt
    28201 aaggtgcctc ttgtgcgcca gaagtgcata taaacgataa cattgacctg tagacttgat
    28261 tatcatggac aaattcgacg ctaatcgccg caaattgctg gcgcttggtg gcgttgcact
    28321 cggtgccgcc atcctgccga cccctgcgtt tgcaacactc tctaccccac gcccgcgcat
    28381 tttgacactc aataatcttc ataccggaga gtcaatcaaa gcggagtttt tcgatggcag
    28441 aggctatatt caggaagaat tggcaaaact taaccatttt ttccgcgatt accgcgcgaa
    28501 caaaataaag tccatcgacc caggattatt cgaccagttg tatcgcctgc aagggttgtt
    28561 aggcacgcgc aaaccggtgc aactcatttc cggttatcgt tctattgata ccaacaatga
    28621 actacgcgcc cgcagccgtg gagtagcgaa gaaaagctat cacactaaag gccaggcgat
    28681 ggatttccat attgaaggta tcgcgttaag caatattcgc aaagccgcgt tatctatgcg
    28741 cgcaggtggt gtaggatatt atccacgtag taactttgtg catattgata ccgggccagc
    28801 acggcactgg tagtaattgc ttaacgaaac aggggcagta tgaactatcg tattattccg
    28861 gtcaccgcat tctcccagaa ctgttcatta atctggtgtg aacaaacccg tctggccgca
    28921 ctggtcgatc ctggcggcga tgcggaaaaa atcaaacagg aagttgatga cagtggcctg
    28981 acactgatgc agatcctgct gacgcatggt catctggacc acgttggcgc agcggcggaa
    29041 ctggcgcaac attacggcgt gccggttttt ggcccggaaa aagaagatga gttctggctg
    29101 caaggcttgc ctgcgcaaag tcgtatgttt ggtctggaag agtgccagcc gctgacgcca
    29161 gatcgttggc tgaacgaagg cgataccatc agcataggga atgtgacttt acaggtgtta
    29221 cattgccctg ggcatacgcc gggtcatgtc gtgttttttg atgatcgggc aaagctgctg
    29281 atttctggcg atgttatttt caaaggcgga gtagggcgca gtgacttccc gcgtggcgat
    29341 cataatcaac tgatttcttc aatcaaagat aaattgctgc cactggggga tgacgtgaca
    29401 tttattccgg gtcacggacc attatccaca cttggttatg aacgcctgca taatcccttc
    29461 ctgcaagacg aaatgcccgt ctggtaaggc acataaaaaa gcccgctttt aatgcgggcc
    29521 tggatttctg gcaaagtgcg ctttgtttat gccggatgcg gcacgagcgc cttatccggc
    29581 ctacaaaatc gtgcaaattc aaaatattgc aggggacgtg taggcctgat aagcgtagcg
    29641 catcaggcaa tgttgcgttt gtcatcagtc tcagcccgct tttcagcggg cttcattgtt
    29701 tttaatgctt acagcactgc cacaatcgct tcgcacagcg gagccatgtt atctggtgtc
    29761 atcccggcca catttacgcg accagaagca accgcatata cgccaaactc ttcgcgcaga
    29821 cgcagcactt gttcttttgt cagaccactg aaggagaaca tgccgttctg tttgatgata
    29881 aagctgaagt cgcggtttgc gcctttttcc tgcagcgtat tgacgaacaa ctgacgcata
    29941 cgctgaatac gctggcgcat atcagtcagc tcttgttccc aaatcgcacg taacgcatcg
    30001 ttgctcagga tggtggcaac aacagaagcg ccgtgtgctg gtgggttaga gtagttagcg
    30061 cgaatcgccg ctttcatttg gctgaatgcg cgatcaacgg tttcactgtc ggcagcaacc
    30121 agagtacaag cgccaacacg ctcgttgtac aggccaaagt ttttagagta ggaactggca
    30181 acaatcagct ctttatgcat agccgcgaaa gcgcgcagtc cttcagcatc ttcttccaga
    30241 ccacgggcaa aaccctggta agcgaagtca aacagcggta accagccttt ctcaactgag
    30301 agttgtgcca gtgtctgcca ttgttccagc gtagggtcga taccggttgg gttatggcag
    30361 cagccatgga acagcactac gtcgccagcc tgagcttcgt tcaggctgtt aatcagtgca
    30421 tcgaagtcaa gagtgtgatt ttccgcatca taataagcgt attcacgaac ttccagacct
    30481 gcagagttaa agacgctctt atggttcggc cagcttgggt tgctcaccca cactcgctta
    30541 acgctggtat tttttgccag gaaatcggca gctacgcgta gtgcgccagt gccccccgga
    30601 gtctgtgccg tgcgagcacg tttgtcattg atcagggcgc tacctttacc aaacagcagt
    30661 tcctgagtgc agcgaccaaa ttcagggatg ccgtcaatgc cgaggtaatt tttggtggtt
    30721 tcattttcga gcagatactg ttcagccttt ttcacgctgg tcagtaccgg ggttttgccc
    30781 gtctcatctt tatagacacc aatcccgagg ttaattttgc cgggacgttc atcggcacga
    30841 aacagatcgg ccaggcccag aatcgggtcg gcaggagcgg cggtaatgtt ctcaaacatg
    30901 acgaggttcc attatggtta cagaagggaa gtccgctatc agggtaacgg gagatttaca
    30961 aaattccaac tattactgat gaaaacgcag gctgtttttg caagacgtga gattgctctg
    31021 gaaggtataa aaaaaacagg accaaagtcc tgttttttcg gcatttaaca aagaggtgtg
    31081 ctattagaac tggtaaacga tacccacagc aacggtgtcg tctgaaccta cgcccagttt
    31141 gttgtcagaa tcgatctggt tgatgatgta gtcaacatag gtggacatgt ttttgttgaa
    31201 gtagtaggtt gcgcccactt caaagtagtt caccagatca acatcaccga taccttctac
    31261 gtctttcgct ttagatttgg tgtaagcgat ggacggacgc agaccgaaat cgaactggta
    31321 ttgcgcaact aacagaacgt cttgcgtttt gttggcgaag ccgctggtgt ttgtaaattt
    31381 attagtgatc ggcgtagcgt tacgggtttc accgtagttc gctgccaggt agatgttgtt
    31441 cgcgtcgtac ttcagaccag tagcccactg ttcagctttt ttaccgttgc caagaggttg
    31501 agcttcttgc aggttggtac ggtcagctgc accataagca ccaacgatac caaagccttc
    31561 gtattcgtag ctgatagaac cgccaacacc gtcgccgtta gaacggcgtg cagtgtcacg
    31621 ctcgttttta cccaggtact gaacagcgaa gttcaggcca tcaaccagac caaagaagtt
    31681 ggagttacga taggtagcaa cgccgccaac acgaccaacg aagaagtcat cgctgtatgc
    31741 agtatcacca ccaaattctg gcagcatatc ggtgtaaccc agtgcatcat aaaccacacc
    31801 gtagttacgg ccgtaatcga aagaaccaac gtcagcgtat ttaagacccg cgaatgccag
    31861 acgcgttttg ttaccagttt gagcgtcagc gccttcagag ttgttaccct ggaagttata
    31921 ttcccactga ccataaccgg tcagatcgga attgatttga gtttcccctt taaaaccaag
    31981 acgggcatag gtcatgtcgc cattgccacc gtaactgttt tcaccgttac ccttggaaaa
    32041 ataatgcaga ccaacagctt taccgtacag atctactttg ttgccatctt tgttatagat
    32101 ttctgcagcg tttgcagtac ctgctactaa cagagcaggg acgatcactg ccagaatatt
    32161 gcgcttcatc attatttatt accctcatgg ttttttttat gacacctgcc actgccgtca
    32221 ataagttctg tcaataaaaa tttacggaac tattgatgag agtttggtgt ctttatgtgt
    32281 ctgcaggcat ctttccattc aaa
//
LOCUS       DAFNBJ010000040        32302 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000040 DAFNBJ010000000
VERSION     DAFNBJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.040"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(49..1446)
                     /gene="ccp"
                     /locus_tag="L2X49_001766"
     CDS             complement(49..1446)
                     /gene="ccp"
                     /locus_tag="L2X49_001766"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBN4796861.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1851..3500
                     /locus_tag="L2X49_001767"
     CDS             1851..3500
                     /locus_tag="L2X49_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBN4796862.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3551..4153)
                     /locus_tag="L2X49_001768"
     CDS             complement(3551..4153)
                     /locus_tag="L2X49_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN4796863.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4673..5572
                     /locus_tag="L2X49_001769"
     CDS             4673..5572
                     /locus_tag="L2X49_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4796864.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5621..6634
                     /gene="yhjD"
                     /locus_tag="L2X49_001770"
     CDS             5621..6634
                     /gene="yhjD"
                     /locus_tag="L2X49_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBN4796865.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7045..8367
                     /locus_tag="L2X49_001771"
     CDS             7045..8367
                     /locus_tag="L2X49_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBN4796866.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8601..10661)
                     /locus_tag="L2X49_001772"
     CDS             complement(8601..10661)
                     /locus_tag="L2X49_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBN4796867.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10731..11498)
                     /gene="pdeH"
                     /locus_tag="L2X49_001773"
     CDS             complement(10731..11498)
                     /gene="pdeH"
                     /locus_tag="L2X49_001773"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBN4796868.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVAHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11730..12659
                     /gene="kdgK"
                     /locus_tag="L2X49_001774"
     CDS             11730..12659
                     /gene="kdgK"
                     /locus_tag="L2X49_001774"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBN4796869.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12755..14251)
                     /locus_tag="L2X49_001775"
     CDS             complement(12755..14251)
                     /locus_tag="L2X49_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBN4796870.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14472..15758)
                     /gene="dctA"
                     /locus_tag="L2X49_001776"
     CDS             complement(14472..15758)
                     /gene="dctA"
                     /locus_tag="L2X49_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBN4796871.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15941..17929)
                     /gene="hmsP"
                     /locus_tag="L2X49_001777"
     CDS             complement(15941..17929)
                     /gene="hmsP"
                     /locus_tag="L2X49_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBN4796872.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18011..21484)
                     /gene="bcsC"
                     /locus_tag="L2X49_001778"
     CDS             complement(18011..21484)
                     /gene="bcsC"
                     /locus_tag="L2X49_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HBN4796873.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLHDC
                     SGNRSQSDSGASVAVGWRNDTWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21466..22572)
                     /gene="bcsZ"
                     /locus_tag="L2X49_001779"
     CDS             complement(21466..22572)
                     /gene="bcsZ"
                     /locus_tag="L2X49_001779"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBN4796874.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22579..24918)
                     /gene="bcsB"
                     /locus_tag="L2X49_001780"
     CDS             complement(22579..24918)
                     /gene="bcsB"
                     /locus_tag="L2X49_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBN4796875.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24929..27547)
                     /gene="bcsA"
                     /locus_tag="L2X49_001781"
     CDS             complement(24929..27547)
                     /gene="bcsA"
                     /locus_tag="L2X49_001781"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HBN4796876.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27544..28296)
                     /gene="bcsQ"
                     /locus_tag="L2X49_001782"
     CDS             complement(27544..28296)
                     /gene="bcsQ"
                     /locus_tag="L2X49_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBN4796877.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPGNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLHYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28308..28496)
                     /locus_tag="L2X49_001783"
     CDS             complement(28308..28496)
                     /locus_tag="L2X49_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBN4796878.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28769..30340
                     /gene="bcsE"
                     /locus_tag="L2X49_001784"
     CDS             28769..30340
                     /gene="bcsE"
                     /locus_tag="L2X49_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBN4796879.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLAHSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30337..30528
                     /gene="bcsF"
                     /locus_tag="L2X49_001785"
     CDS             30337..30528
                     /gene="bcsF"
                     /locus_tag="L2X49_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBN4796880.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30525..32204
                     /gene="bcsG"
                     /locus_tag="L2X49_001786"
     CDS             30525..32204
                     /gene="bcsG"
                     /locus_tag="L2X49_001786"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBN4796881.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
BASE COUNT         7595 a         8875 c         8145 g         7687 t
ORIGIN      
        1 atcgtaataa aaatcgccca aacattcctg ttcgggcgat caaattaatt attgtttatc
       61 ctgcatatac ggcgtgtaca ccccgttcag actgtgcagg aaagctacga tatcatccac
      121 atcctcctgc ggcagctctt tgcctacctg atagcgcagc atcagtttca ccgccccgtc
      181 cagcgtcggc acgtcaccgc gatggaagta cggtgccgtt aaagcaacgt tacgtaaacc
      241 gggtactttc tgacgcaatt tatcacgctc ttctttagtc acattcatac gaccaatatc
      301 cgccgccgta atttccccaa agttaaagtc ttttttcagc cccaacggtt caaaggaacg
      361 tccgccgaga ataataccac catgacaagt tgcacattta ttatctttaa ataattgata
      421 gccttttttc tgttgcgccg tcagcgcatt ttcatctcca cgcaaccatt tatcaaatgg
      481 ggaatccggc gtaattaatg ttttctcaaa ttcagcaatg gcatcagtaa tattttcgcc
      541 actgaaacct tgcggataaa cttcgaggaa ctgcgcttta agctgcggat ctttttccag
      601 cttagcaata atttcgtccc aggatttcga cgccatttca atcgggttca acggcggtcc
      661 acctgcctga tcctgcaatg ttgccgcacg accatcccag aactgctcaa cgttaaatac
      721 tgagttaaat accgtcggcg cgttaatcgg cccaactgcg ccaccaacac caatcgatgt
      781 ttttctgcca tcgacgcccc ccgcattcaa cgcatggcaa tgagcgcatg aaatggtgct
      841 atcagccgat aaacggggat cgtgatacag cgcaaagccc aacgccactt tttgcgcatc
      901 ggtaggcagt ttttgcggga tgggctgcac cggttcattg cgatgctccg gagcagtatc
      961 attgctggcg taatattccg cgcgctgttt tgcaatccag gccagtattt ccgcccgctc
     1021 ttcatcactc accttacccg cccagtgtag cgcggtataa cgcgttggtg gcatagtttc
     1081 atactgcatc acccattcaa tcttattcag atcgctttgc gaaacgggtt tatcagccag
     1141 cagtgccgca cgcacggcct cgaggttaaa agatttatat ccaagcttaa tgtcgtaatc
     1201 catcaactgt ttcgcgccag gaatataata ataggcgggt aattctgccg aaggtgtgtg
     1261 gcaatagtcg catccttttt cgcggagaaa gcctaaaacc ttattatttt cactgacagc
     1321 agatgcctga acatcggcct gtttactgcg tttattatcg tggtaccaca cataaccaga
     1381 taaccctaaa tagcaaatcg cgacgccagc caggccgatc gcggtaatac gtgagaccat
     1441 tttcattatt cttccctcac ggtggtgagt tattgtgacc cagaaaattt tccagaatgc
     1501 tgcaaacgtc tctgcgagac aaagccatca taaaaaacag ggagatacgc tttttgatag
     1561 gaatcaatca attacatgaa tataaactat caataagata gcctgtgcct attacccaga
     1621 tcaggaatgg catatcatga ttgcggcgag agcaggattg aatgttctga aaatgaaaaa
     1681 caacacgaaa tccgtatttt tataaattcc atggatatta gtgcgttata ttttttgaca
     1741 ggttaataaa cgtgatccac cgcacgcttt gtcgcccacc aggcggagcg aatgactacc
     1801 cttaaagaaa agcccgataa ttagcgacga atttcggagg ttggatcctt atgctcaatc
     1861 agaaaattca aaaccctaat ccagacgaac tgatgatcga agtcgatctc tgctatgagc
     1921 tggacccgta tgaattaaaa ctggatgaga tgatcgaggc agaaccggaa cccgagatga
     1981 ttgaagggct gcccgcctct gatgcgctga cgcctgccga tcgctatctc gaactgttcg
     2041 agcatgttca gtcggcgaaa attttccccg acagtaaaac ctttcccgac tgcgcaccca
     2101 aaatggaccc gctggatatt ttaatccgct accgtaaagt gcgccgtcat cgtgattttg
     2161 acttgcgcaa gtttgttgaa aatcacttct ggctgccgga ggtctactcc agcgagtatg
     2221 tatcggaccc gcaaaattcc ctgaaagagc atatcgacca gctgtggccg gtgctaaccc
     2281 gcgaaccaca ggatcacatt ccgtggtctt ctctgctggc gctaccgcag tcatatattg
     2341 tcccgggcgg ccgttttagc gaaacctact attgggattc ctatttcacc atgctggggc
     2401 tggcggaaag tggtcgggaa gatttgctga aatgcatggc cgataacttc gcctggatga
     2461 tcgaaaacta tggtcacatc cccaacggca accgcaccta ttatttgagc cgatcgcaac
     2521 caccggtttt tgcgctgatg gtggagttgt ttgaagaaga tggtgtacgc ggtgcgcgcc
     2581 gctatctcga ccaccttaaa atggaatatg ccttctggat ggacggtgca gaatcgttga
     2641 tccctaatca ggcctatcgc catgttgtgc ggatgccgga cggatcgctg ctcaaccgtt
     2701 attgggacga tcgcgacacg ccgcgtgacg aatcctggct tgaggacgtt gaaaccgcga
     2761 aacattctgg tcgcccgccc aacgaggtgt accgcgattt acgcgcggga gcggcctcag
     2821 gttgggatta ctcttcccgt tggctgcgtg atactggtcg tctggcgagc attcgtacca
     2881 cccagttcat ccccatcgat ctgaatgcct tcctgtttaa actggagagc gccatcgcca
     2941 acatctcggc gctgaaaggc gagaaagaga cagaagcgct gttccgccag aaggccagtg
     3001 cccgtcgcga tgcggtaaac cgttacctct gggatgatga aaacggcatc taccgcgatt
     3061 acgactggcg acgcgaacaa ctggcgctgt tttccgctgc cgccattgtg ccgctctatg
     3121 tcggcatggc gaaccatgaa caggccgatc gtctggcaaa cgccgtacgc agccggttac
     3181 tgacacctgg cgggattctg gcaagcgagt acgaaaccgg tgaacagtgg gataaaccca
     3241 atggctgggc accgttacaa tggatggcaa ttcagggatt taaaatgtat ggcgatgacc
     3301 ttctgggtga tgaaatcgcg cgcagctggc tgaaaacggt gaatcagttc tatctggaac
     3361 agcacaaaat gatcgagaaa taccatattg ccgatggtgt tccccgcgaa ggcggcggtg
     3421 gcgagtatcc gttgcaggat gggtttggct ggactaacgg tgtggtacgc cgtttaattg
     3481 gtttgtacgg cgaaccataa tatttttaca gccagccgct aacttcctgc tggctgtaaa
     3541 attatcctct tcaggaggag atatttaaca tcattgccgc ctgggtgcga tttttcactt
     3601 ccagacggcg atacagggat tccagatgcg cttttaccgt tccggtactg atattcagcg
     3661 ctctgccgat ctccttattt gattcgcccg ccgctaacat ggttaaaatc tcccgctggc
     3721 gggcgcttaa cgatttgaga tctttaatgt ccttttccgg cgtcgtccgc cagtctccag
     3781 gcagaaacat catccccatc gccgcactat ttaccgccaa cgcaaatgtc tcgacggttg
     3841 aatcacgagg cacaatggcc agcacattaa aatgaataac ttcctgtaac caccgtttat
     3901 tgcaatccgt cgccgtgatt aacaccttaa cctcaggaaa ctgcaccacg gttttttgca
     3961 gcaaccagta gcaaaactca ccatcctgat cgccatcgag cataactaag gcttcagggt
     4021 aactttccag cttttgccat aactcgtctg cctgactggc cccctgaata ctcactcctg
     4081 gaatacgctg ctgtaaactg attttcattc catgaataaa tattgactgc ctgtcaaaca
     4141 tgactatttg cataactgaa tctccacctg aatacgttaa aaagacttaa gtagtggaag
     4201 ggtattaccc gcgagaaaaa ataagaattc gccatttggc ggtggccatt ctacagagat
     4261 gacgcgtaga aaatagttac cgatataaat agttacagct aaacgcctga aattacatgt
     4321 cgagggcact atttaaaaca attttgagga tttccttata ttggtggtta gtacgcatgc
     4381 aattaaaaat gaaactccgc gaccacaagc aaaaataaca aacggcaatg agacaaaaat
     4441 aagcacaaat agccaacacg tcctctattc actttaaagg gaatcgctga aaaatacgct
     4501 ctgtttaggg ggattcacct ttctcagaaa gctattccgc ccttttcctg ctgagaaatc
     4561 gccacattcg tcatgacaac attgtgaaac ccggcattag atgttagaaa aaacaataaa
     4621 caatgcgata tgcgcgttac tgtttcgctg tgaacaacaa tcggtcaaag aaatggataa
     4681 aattcacgca atgcagttgt tcatcaaagt cgcggagctg gaaagttttt cccgcgcggc
     4741 ggatttcttt gctttgccaa aaggaagtgt ttcgcgccag atacaggcac tggaacatca
     4801 acttggcacc cagcttctcc agcgcaccac gcgacgggtc aaactcacgc cagaaggcat
     4861 gacctattat caacgagcaa aagatgtgtt gagtaatctc agcgaactgg acggtctgtt
     4921 tcaacaggat gccaccagta tcagcggtaa attacgcatc gacatcccgc caggaatcgc
     4981 gaaaagcctg ttactgccgc gcctgtcgga atttctctat ctgcatccgg gaattgagct
     5041 ggaactgagt agccatgacc gtccggtaga tattcttcat gatggttttg attgcgtgat
     5101 acgcactggc gcgttaccgg aagatggcgt tatcgcccgt cccctcggca aactgaccat
     5161 ggtcaactgt gccagtccgc actatctgac gcgctttggt tatccgcaaa gccccgatga
     5221 tctgacttca cacgcaatag tgcgttatac accgcacctg ggtgtacatc cgttaggttt
     5281 tgaggttgcc agcgttaatg gcgtccagtg gtttaagtct ggcggcatgt tgacggtaaa
     5341 cagtagcgaa aactatctcg ccgccggtct tgccggtctg gggattattc agatcccgcg
     5401 cattgccgtg cgcgaagccc tgcgtgccgg gcggcttatt gaagtgttgc caggctaccg
     5461 tgccgagccg ctctcccttt cgctggttta cccgcagcgt cgggagcttt cccggcgtgt
     5521 aaacctgttt atgcagtggc tggctggcgt aatgaaagag cacctggact gaccgactat
     5581 actttttaag aatgaccacg acagaaggac aaaagagcgg atgacgcagg aaaacgagat
     5641 caaacgtccc acccaggatc tggagcacga gccgattaag cagttggata atagcgagaa
     5701 aggcggcaaa gttagccagg cgctggaaac cgtcaccacc actgccgaaa aagtccaacg
     5761 ccaaccggtt attgcgcacc tgattcgcgc gacagaacgc tttaacgatc ggctgggtaa
     5821 ccagtttggt gcggctatca cctatttctc atttttatcg atgatcccga ttttgatggt
     5881 gtcgtttgcc gcagggggct ttgtgctggc ctcccatccg atgttgctac aggatatctt
     5941 cgacaaaatt ctgcaaaaca tcagcgatcc gacgctagcc gccacgttga aaaacaccat
     6001 caacaccgcc gttcagcagc gtacgactgt agggcttgtc ggcctggcgg tggcgcttta
     6061 ttctggcatc aactggatgg gtaacctgcg tgaagcgatt cgtgcccagt cgcgcgatgt
     6121 ctgggaacgc tcgccgcaag atcaggagaa gttctgggta aaatatctgc gtgattttat
     6181 ttcactgatt ggtttgttaa ttgcgctgat tgtgacgctg tcgatcacct cggttgccgg
     6241 ttcggcgcag caaatgatta ttagcgccct gcacctgaac agcattgagt ggctgaaacc
     6301 gacgtggcgg ttgattggcc tggcgatttc catcttcgcc aactatctgc ttttcttctg
     6361 gatcttctgg cggttgccgc gccaccgtcc gcgcaaaaaa gccctgattc gcggaacatt
     6421 tctggctgct attggttttg aagtgattaa aatcgtgatg acctacacct tgccatcgct
     6481 gatgaaatcc ccctctggcg cagcatttgg ttccgtgctg gggctgatgg catttttcta
     6541 cttcttcgcc cgtttgacgc tgttttgcgc ggcgtggatt gccaccgccg aatataaaga
     6601 cgacccgcga atgccaggga aaacgcagcc ttaaaatagg ccggatgcgg cgtaaacgcc
     6661 ttatccggca tacgatttta ttcggcctgg ctccccgtag gccggataag atgcgccagc
     6721 atcgcatccg gctataatgc gcacataacc tcttgaaact cttccccaga gcctcttcag
     6781 ccatctattt gggagcaaac aatttcattc caactcataa ccccagcata taaatccagt
     6841 tggtaacttt tatttaacct gaaaccagtt ttatccacta tttataaaat tatgtgaagc
     6901 atttcataga agaaaaatca ctggcctaaa cattatcccc tttttgcctg gtttttgacc
     6961 atttccgcga tttgttacac attgaaatat cacttttgct gtgcgtaata tggctattcg
     7021 ttagccaaaa aataagaaaa gattatgcaa gcaacagcca caacactcga ccacgagcaa
     7081 gaatacacgc caatcaactc gcgtaataaa gtccttgtcg cctctctcat tggcacggcc
     7141 attgagttct tcgactttta catttacgcc actgcggccg ttattgtgtt tccgcatatc
     7201 ttcttcccgc agggcgatcc aacggcagca acgctacagt cgctcgccac cttcgccatc
     7261 gccttcgtcg cgcgccccat tggctctgcc gtttttggtc attttggcga tcgcgttggg
     7321 cgtaaagcga cgctggtcgc ctcgttgcta acgatgggga tttcgaccgt ggtgattggt
     7381 ctgctgccgg gctatgccac gattggtatt ttcgccccgc tgctgctggc gctggctcga
     7441 tttggtcagg gtctgggctt aggcggtgaa tggggcggcg cggcgctgct ggcgactgaa
     7501 aacgccccac cgcgcaaacg tgcactgtat ggctccttcc cacagctggg cgcaccgatt
     7561 ggcttcttct ttgccaacgg cactttcttg ctgctttcct ggctactgac cgacgagcag
     7621 tttatgagct ggggctggcg tgtgccattt atcttctcgg cggtgctggt cattatcggc
     7681 ctgtatgttc gcgtgtcgct gcatgagtcg ccggtgtttg agaaagtcgc taaagcgaaa
     7741 aaacaggtga agatcccgct gggtacgctg ctgaccaaac atgttcgcgt aaccgtactg
     7801 ggtacgttca ttatgctggc aacctatacg ctgttttaca tcatgacggt ctactctatg
     7861 accttcagta ccgccgccgc gccagttggg cttggcctgc cgcgtaacga agtgttgtgg
     7921 atgctgatga tggcagttat tggttttggc gtgatggtgc cagtcgccgg attactggct
     7981 gatgcctttg gtcgccgtaa aagcatggta atcatcacca cgctgatcat cctgttcgcg
     8041 ctgttcgcct ttaacccgct gctcggttct ggcaacccga ttctggtttt tgccttcctg
     8101 ctgctggggt taagtctgat gggactgacc ttcgggccga tgggcgcgct gttaccagag
     8161 ctgttcccga cagaagtgcg ttacaccgga gcatcgttct cttacaacgt agcgtcgatt
     8221 ctcggggctt ccgttgcacc atatatcgca gcctggttgc aggctaacta cgggttaggt
     8281 gcggtggggt tatatctggc ggcgatggct ggcttgacgt taatcgccct gctgctgacc
     8341 catgagacgc gacatcagtc gttgtaagta tcgtgtgggc agccaccctc accccagccc
     8401 tctccctgaa agggagaggg agaaaagcgg agcgagatgt tatatcgcga acataacagc
     8461 cctcccccag aaagtgaacg gaaaagcggt actcgttgtt tcaacacgca caaaacagtc
     8521 ccctcgcccc tttggggaga gggttagggt gaggggaaca cgctgctccc gaactcacca
     8581 gcaaacacaa tccaacctca tcacttcttc atctgcgaca atatcgtccg acactgatta
     8641 gcctcccctt cggaaggtga gatcagtgcc agtaacgccg ccgccggtgt taccagcgtt
     8701 gccagtgccg cagcaacagc accacgggca atcaacggcc cggctttcac cccagcctgc
     8761 ggatttttaa acgtcccacg cacatacagc ggcgaacgca gtgtgataat ccgaatccct
     8821 ttactctccg gatcaatagt caaatccagc tgttccgaag caaaacttgc cgtgccggta
     8881 acattaatca acgcgttctc agtatcgaaa gcaaaaatct gcgggcgcgc cacgccgttg
     8941 gcaatattca gattcgccgc cgcgcagttc acccgcacct catcgtcacc aaatatcgca
     9001 ccgacaatgt agttgccgac attcagccca acaatctcca tcaggttgcg gctcaccagc
     9061 ccgtcattca tcaacagttt caggttgccg ttactgttgc ctaaaagtgc tgccaccgag
     9121 ttaccgctac cgcgtagttc cgcgtcaccg ttcatttccc ccagcgtctt ctgcatcagt
     9181 tccacatcgg gcatcagttc tttcagtttc agtcgacgag cctgaatatc tgcccgcccc
     9241 tgcatcggct ttttatcgcc ttccagatga atattcgccg caatgctgcc gcccgccatg
     9301 ccaaatttca gcggttgcag gcgcaggtca gcatttttga ggatgatatg agtagaaaga
     9361 tcgctaatcg gcaggctact gccatgctca atgcgccgcc ctttgaagcg aacatcggca
     9421 tccataacgt cccatttatc ggtttcgaag cggtcataag gcagcacttt gcccgcaggc
     9481 tgaacgcttt tttcgccctt cttctgttca gaccgtttcg acttttctgc ccctttcccg
     9541 gagtcaacgc caattaacgg tcccaggtcc gccagccgca attgccgcga ctcgacatca
     9601 ccttccagtt ttggtcgtgg ctttccggtg gtgtagacca gagaaccgtg gatatcgcta
     9661 tcgccaattc gcccattaaa accgcgataa tcaaagaccg acgatttttc agtgtcgatt
     9721 ttcgctacca gccgaccatc cgtttcaaac ggcggggtat cgggcagcag aacgcccgtc
     9781 agttcataga gatcacccag tgaatcgcca gaaaatttaa gccgtaaatc gacaccgccc
     9841 atcttcattg ggtcattcac gacgccatca aaagcaacac gggtgttacc agagcggaaa
     9901 tcagcctgta ccggaaacgg cgtcccttcg ccacgcagcg ccagcatacc gcctattttt
     9961 cccgtacccg tgagcggttc accgttataa cgtccctgcg ccttcaggcc aaaaacgtaa
    10021 tcgcccacct tttctttatc cgctttacct ttcgatccag taacttcgct gaacggcagc
    10081 ggcttgccta acggatcaac aaaaatctcc agatccgctt tgcttacttt gtcatcaatg
    10141 gcgatccgcc cttgatcgaa aagaatatta tccagccgaa acgaccatgc cgacggcttt
    10201 gcattcgcgt ctttgttatc atcgttggcg agattaaacg tccagttatt gttcttttcg
    10261 gagaggcgaa tcaggcgcgc gtcgggcttt tcgagcttga tccacggcag ccagaccgtt
    10321 ttggtcagca gcgccagcgg ggccagcgtt gcctctacgc gtggcaaatg caccatcgtg
    10381 acttcgggaa tatccggtgg attgccaaga atgatgtctt ccgcgtgtac atggggccac
    10441 ggcacccagc tgcgccagcc tgtttcttgc ttttgccgct cccacaccac gcccagatcg
    10501 ccacggatag cgaacggacg attcaactcc gcagagactt tctggttgat ggtcggtttg
    10561 agtcgattcc agtcaaaagt tgcaatcaaa atgatcgcca cgacaatcaa caacaagaaa
    10621 gcccctgaaa tcgcagcggt tattttgcct gccttgctca tcgttcgctt cctcatattc
    10681 ttcctgtgcc agtcctaaag atagtccagc caggcggaaa atgaggcagc ttatagcgcc
    10741 agaaccgccg tattcagcgt ttctatcggt gccgggcgtg aaagaaacca gccttgtgcg
    10801 gcgaatgcgg gcgagttctg aacatcacgc cactcttccg gcgtttctac gccctcaaca
    10861 atcaccccgc gacaatagcg attcattaga tgtaaaagct gagaaaagag tgtgcgtcct
    10921 tccggcgact gacgcagcat cacaaacagt tctcgcgcga ttttgatgta gtcataacgc
    10981 acttcactta gtgcagagaa atttgccatc ccggtaccaa aatcatccag ccacagcggg
    11041 ccaaattcac acatcgaggc aaaggttgaa tctttcggca gacggatatg ctccaccagt
    11101 tcgaaacgca gccagggaag acgctcaatc tggcgcagga tttttggttg ctgacgcagg
    11161 gcgatgagcg tagggccatc aatattgacc gatgccagca ggccgtgctc tataaagaag
    11221 tcggcttttt gcgccagcaa atcaatctgc tctttcacaa cctccatccg atggctgacg
    11281 gtgatttcag taaaatagcg atccggcggc aggcgttgcg aagggttcaa gggatgcgcg
    11341 accaccgtta atagctccac ggccattaac cgcccgcatg tttgatagat cggctgccag
    11401 gtgtaagcac gctcacactg caaccaaaaa cgccgttcct gcaagctctc gatgcttgct
    11461 tcagggttgc ttattcgctg gataacctgc cttatcatca aagatgtcct gtgtttaaaa
    11521 gtgatgctgc ccggactcga caaagattat cggcgcgtaa gggcagaact ttaccgcctg
    11581 gccggggtaa atttcctcac tccacaacgg aatctttcag gaatacgtcc tggctcaaaa
    11641 aaataatgga acactgtttt aatatggttg accagcaaac caccacagcg caaactaacg
    11701 ctaatttttt acagatcagg ttcacgacta tgtccaaaaa gattgccgtg attggcgaat
    11761 gcatgattga gctttccgag aaaggcgcgg acgttaagcg cggtttcggc ggcgataccc
    11821 tgaacacttc cgtctatatc gcccgtcagg tcgatcctgc ggcattaacc gtccattacg
    11881 taacggcgct gggaacggac agttttagcc agcaaatgct ggacgcctgg cacggcgaga
    11941 acgttgatac ttccctgacc caacggatgg aaaaccgtct gccgggcctt tactacattg
    12001 aaaccgacag caccggtgaa cgtacgttct actactggcg taacgaagcc gctgccaaat
    12061 tctggctgga gagcgagcag tctgcggcga tttgcgaaga gctggcgaat ttcgattatc
    12121 tctacctgag cgggattagc ctggcgatct taagcccgac cagccgcgaa aagctgcttt
    12181 ccctgctgcg cgaatgccgc gccaacggcg gaaaagtgat tttcgacaat aactatcgtc
    12241 cgcgcctgtg ggccagcaaa gaagagacac agcaggtgta ccaacaaatg ctggaatgca
    12301 cggatatcgc cttcctgacg ctggacgacg aagacgcgct gtggggtcaa cagccggtgg
    12361 aagacgtcat tgcgcgcacc cataacgcgg gcgtgaaaga agtggtggtg aaacgcgggg
    12421 cggattcttg cctggtgtcc attgctggcg aagggttagt ggatgttccg gcggtgaaac
    12481 tgccgaaaga aaaagtgatc gataccaccg cagctggcga ctctttcagt gccggttatc
    12541 tggcggtacg tttaacgggc ggcagtgcgg aaaacgcggc gaaacgcggg cacctgaccg
    12601 caagtaccgt tattcagtat cgcggcgcga ttatcccgcg tgaggcgatg ccagcgtaaa
    12661 agcagcatat gcaaaaggct ggataaagtg ttacccctct atccagctca tccataaata
    12721 cctgatgatt gtgctaactc atcaccatca ggcattactg tgcaggtgga atatccgtca
    12781 cttcaggatg tacgtcatcc gtggcgacag aggtggtcgc agcggcggta gatggggcca
    12841 tgatttgatc ccaggccgcc tgcaacgctt tcatgttaaa ttccggctcg cctttcggct
    12901 gcagcagtat taacgccata tcattcgaca actgctgacg cagatcctga tttaacatct
    12961 ccaccgtcag gctgttaagg aaatcctgcc gcagtttctg atactgctcc ggcgcgatat
    13021 cgacgacctg attttgcaac gaacgcatcc gctgacccat cagaatatcg gtatcagccc
    13081 gcgcataggc ggcaaacagt ttctgcagct ccagtttctt ttgcgccact aacgcattga
    13141 actcttcttc cggcagacct ttatcgcgaa ccttcgccag ttcacgcgcc accagattaa
    13201 ggttgctgtt cagcttgtcg tttggtgatt cgatgttgat ggcacactgc gcacgcagat
    13261 acagcacacg gcagtcaaat ccaagaccga tgtctttgct gttactggcg cttaacgctt
    13321 gctgaacatg ccagaacagc gcctcgcggg ccaggtccgc acgccagtag cgcagcagtg
    13381 cggctgattc acgaatcggc tgccacggcg tatcccacat gatagataac cggtcctgac
    13441 gcaccgcgtc agtcataata ctcaccgctt ccgcacgcag cggagaaagc gtcggcaccg
    13501 gagctggcgt ttcacgtttg cctttcagtt cgccaaacgt tttgttgatt tggtcgacaa
    13561 ccgagcgcgc atccacgttt cccaccacca gtagcgtcat tgcatccggg gtgtaccatt
    13621 tctgatagaa atctttaatt ttttccgctt caacgggttg tttcagcgga tcggcaggat
    13681 cgtgacctaa caaggttgat cctttcagac gataacgcca ccagccctct ttagtatcgg
    13741 caggccaggt tgccaccatg tcctgacttt gcagcgcgtg gttgatggtt tctggcgtga
    13801 tagtcagttt gccagtggca tttgccaggt aagagagcgc ctctttcagc aaatcgttac
    13861 ggttattggg caaactcaga ttaaacagcg tagtgtcata cgagacaatt accggcggca
    13921 tcgggcgttt agggtcgatc ccctgctgcc acaatgaacg cgcctgtgct gcgtcaaggc
    13981 caccgctttg cgttagcgca atacgaggga tggcgtgact gtaaccgctc tgttgtgtac
    14041 tttcggcgag cgaaccggta ttaaccagca ggcgaatttc aacacgatcg ctgggacgct
    14101 ggggggtggt cagcacttgc cactgtaaac cgttggaaag cgtcccctgt tgccatgctg
    14161 gatcaggctg gagcgcatct gcctgcacat agccagcagt ggccatcatc agcaaaccgc
    14221 ccgctaaaag tcgaattttt gtgccctgca tgtgaacccc tgatcaacta tcctggtaat
    14281 aaaaaaactg cccgttgtcg gtcagtctaa atgacgttaa aaacacttcg tgttagaccg
    14341 caagaacatg aaaatgtcac ggaagaagtg aaataaacct gaactcaccc aggtggtaag
    14401 ttcaggcaca ggggtcaatt atgcgcaaac acccgcactc ggggaaggga gtgcgggcat
    14461 aagtgatgag attaagagga taattcgtgc gttttgccat ccggcgcacg attattcagc
    14521 acatcgtcca gttttttgtg gtccagttct ttcacccact tagcaacgac aatggtcgct
    14581 acgccgttac cgaccaggtt agtcagcgca cgagcttctg acataaagcg gtcgataccg
    14641 aggatcagcg ccagacccgc taccggcaaa tggcccaccg cagagagcgt cgccgccagc
    14701 acgataaagc cactacccgt tacccctgcc gcccctttag aagaaagcag caacacgatt
    14761 aacagcgtga tttggtggac gatatccatc tgactgttag tggcctgggc gataaacacc
    14821 gccgccattg tcaggtatat cgatgtgcca tcaaggttaa acgagtagcc tgtcgggatg
    14881 accagcccca ccaccgattt acggcagccg agtttctcca tcttgtcgag catacgcggc
    14941 agcgccgact cggaagatga agtccccagt acaatcagca gttcttcacg gatgtagcgg
    15001 ataaatttga agatactgaa accagtcgct ttagcgattg aacccaatac cagcaccaca
    15061 aacaggatac aggtaatgta gaaacagata atcagctgcc ccagttgcac cagtgtgccg
    15121 acgccgtatt taccgatggt aaacgccatt gccccgaacg caccaatagg cgccagacgc
    15181 atgatcatat tgatgatgcc gaagatgacc tgcgagaaac tttcgatgac gttaaaaatc
    15241 agttggcctt tgctgcccag acggtggagc gcaaaaccaa acagtacggc aaacagcagc
    15301 acctgcagaa tgttaccgct ggcaaatgcg ccaatgacgc tcgccgggat gacatccata
    15361 atgaaggcga caatgccctg gtctttcgcc tgatcggcgt aaaccgctac cgctttcgca
    15421 tcaagcgttg ccggatcgac gttcattccg gcaccaggct gcacgacgtt aacgatgata
    15481 agaccaataa tcagcgcgat ggtactgaca atttcaaagt aaagcagtgc gactgcgccg
    15541 gtacgaccga ccgccttcat gctttccatg cccgcaatgc ccgttacgac ggtacaaaag
    15601 atgacaggag cgatgatcat cttaatgagc ttaacgaagc cgtcgccaag cggtttcatt
    15661 tgctcgccta tttcaggata gaaatggcca aggagaatac caatggctat cgctgtcagg
    15721 acctgaaagt aaaggctttt aaacagagag gttttcatag ggtgtccttt agtaaaacca
    15781 caggtcttgt aaggttatgg ggtacctgcg gccttaaaat aacacccaga caacatcaca
    15841 gaaatgtacc tggatcataa ttgaaacaaa aaggttaaaa agtttgagct ggctcgcaca
    15901 aaccagcact ttttaaagtt ttgtaatcag tttggggtag ctacttttct tccaggtaac
    15961 tctcttcgaa gatttcaata gggagtgggc gagcaaaaag gaagccctgg gcaataccaa
    16021 cgcccgcttt tgccagccag tcgcgttgtg cttcagtctc cacgccttcg gcaatcattt
    16081 gtaagttcag gctctgcgcc agcatgataa ttgcagcaat catgctgcta tcttccggca
    16141 agccttcaac aaacattttg tcgattttca gtacgtcgat tggcaacgat ttcatatgct
    16201 gcagctgacg cagccctgcg tagcccatgc cgaaatcatc cagcgccacc cgaactccgg
    16261 cattgcgcag cggacggagg attgccaccg cagcatgagg gtcgtcaata cgtcggcttt
    16321 ctgtcacttc cagaatcagt gttcccggct gaatgcgata gcgggttaac agttccagca
    16381 tatccgccac catatttggg tgcatcagtt gcagcgcaga gaggtttacc gacaagggca
    16441 gcataatgcc gcgctcttgc caggctgcaa gcaatcgaca ggactcttcc agcacccagt
    16501 gaccgacggt aaccatcagc ccacagcact caatgcgatc gattaagcca tccggcaggt
    16561 cccaactgcc atccggttgc tggatacgca gtaacacttc cgcactgacc agttttccgc
    16621 tggtcatctc gacctgtggc tgtaaccaaa tagcaaactg atgattttcc agtgcattaa
    16681 ggatatcgct ctcttccgtc aaccgcttct gggcggcttc catctgctgc ggatcaaaga
    16741 actgaatctg attcttgcct ttatggcgag cggtaaatgc cgcagaaata gcgcgactgt
    16801 aaagctgttc ggcggtgaga tcgccgtaga acatcgccac gccaatgcta cagtgcggac
    16861 ggagttgaat acgttcaatc ggcaggcgct cgctcatgat agtgagcact tgctgaccta
    16921 aggtgattgc gtgccacggt tcctgtacac cgttggcaat gacagcaaag tcataaccgc
    16981 taatctgcgc gaggatcata cgtggcgaca gtaccgattt gagtttttcc accagcgtca
    17041 gcagcagaat ttctcgttgc gcctctttca gcacgcccgc agtatcacgc agggtttcac
    17101 aggtgatgat catcagcgcg gtggtttgtt tacgcgcgac aacctgctcc agcatctcca
    17161 tcagcaaggc tttgttcggc aaatccgaca ccgggaagcg catcgcattt tcgttctgtt
    17221 cttcataatg gcgctgcagc aattgctggt tgaggttgta actgcgcacc aacataccga
    17281 tttcatcgtc ctgatgcaga cgcggtaatg ccagttggtg accaacaagc tcctgggctg
    17341 ggatggcgtt aagttcgcga gcaatattgc gtaacggatg caaaatcagg cggttaatgc
    17401 accagctaat cgccaccgtc aatattaacg acaaaagtaa gtaaatggtc actaacgttg
    17461 agagggtgct catcacgaac ttatacatac ggaaggaatc cgcctgtagc accagatagg
    17521 caattggctg cggatttgcc ggacgttcga gcgagtagac gcccagcgag atttgaaccg
    17581 gtagctcaaa caggcgagta accattaccg gcaccggacg ctctggaata aaacttttac
    17641 gcagcgcctg gaactggtta ggcagcacta catcggcacg gctgaccacg cctgccggtt
    17701 taatgctggc aagaatagct tccgcttcgg gaatatcgcc tttcaaaata gctgaagata
    17761 agggttcgcg gacagagcga gcaatgcttt ccagttgcgt agccgtgtta tagcgattct
    17821 gctggaccag atggaacagc aaaacggtgc aaaaaataaa aacgaacacc aggacaacgg
    17881 ctgccaccat tgccatctgc ttgattgtta acgagcgact tacgcgcaaa ttgtctcccc
    17941 ccggaaatcc cagattgccg cccgagtata cccgatcgca gcggcattaa gagaggcgct
    18001 atctgaaaac ttaccagtcg gcgtaaggta tcagcggctg cggcggtaaa tccatgtcac
    18061 cctgccatcc ggcggcggaa taacgtacgt agagcaggaa atggctgggt gcgtaatctt
    18121 tcgcctgctg gatatcaatt gccgtgccaa caaaccagtt ggaagtaaca cgtcgttcaa
    18181 gtaatgcccg cgccgtgtag ccgaagccct gactgctgcc gccatcgttg gattgccgcg
    18241 cagcttcttc ctgccagtcg gtcgggatca gattcatcag cggataacgc ggcatggttt
    18301 tggtgcgtga atgcgaccac gagccagacg cccccagctc ccacgaccag ttttccgtgc
    18361 gctcccgcca catcaccggt atggcaaacg acaggtattc ctgcggactg tagtaaccgc
    18421 cctgaccgag tgagtagcca ctcaggtctt tgtcgtaatg ccagatcatg ttgttcaggc
    18481 cgattgtgac gcggcgattg ttctggttaa tgaccttata gtaatagccc gtcatccagc
    18541 gcacgcgcca gttatcttcg acatttttac cggttaactg gtcgccacta agcgatgccc
    18601 agacgccgtt tgcttcacct ttatcgtagc tcagacttag ccccacaccg tcggcacgta
    18661 cgccacccca ttttttcccg gtattgctcg gggagtcttt ttgcccgcca aaggcaagca
    18721 aagaactgga gatgggccga cggtgggcgt taacggtgta acccagcggc ccgatatcat
    18781 cgctgtaact gatgccgccg accacatcca ccacgttgaa gcccatcggc gtggtgccga
    18841 tatcccagct ccaggtgtcg ttgcgccagc caaccgccac gctggcaccg gaatccgact
    18901 ggctgcggtt gccactacag tcgtgcaacg tacaggtacc ccagttgtca tcccatttgc
    18961 catcggcatt agtggagaaa ctgccgacgt tcatattgac gaaatcactg cgaaagaaca
    19021 tccgcccgtc agaatacggc gcatccacct gcaacatggt agtgtgcgct ttcagatcgg
    19081 agtaaccacc ggtgccgctc gacccccagt aatcatgctc gagggtgacg ttaagatcct
    19141 gctggcgata gaggtccgcc gcatcgctgc gcacgccgcg tttcagccag tcatctttct
    19201 cgtcgttacg ggtcagtcgg gtaaaggtgt cgttatcctg cggacgcgtc gtagtcacac
    19261 cggatgcgac catggcgtct ttgtaggttt ccagcgcctg ctttggatcg cccgcctgcg
    19321 cttcaaactt cgcaccatca cgcagcacca tcgcgctttc catcgacggt ggctgagatt
    19381 ttgcctgcgg gatcaactta ttaaacgtcc gctgcgctgc tgcggtatcg ccaagctgcg
    19441 cctgtgccag cgccacgcgc cgctgtgtgt tcagcgaggc gttatcggta gcgggcagtt
    19501 tcgccagctg gctacgtgcc gccgctttgt caccggcagc aatatccact tccgtcagac
    19561 caagaatggc gtcggcgtta gctggctccc gcgtcaggac attctgatat gcagcgcggg
    19621 cggcggtgta atcacgtcgt tgttgcgccc agtcagccag cgtgaggtca atacgcgtgg
    19681 aaggtggttg ctggcgcagc atcgcttccg cttcagcttc tttgccgctt tctcgcaggc
    19741 gattagcggt ttccagcacc tgatcgcttt gcagtcgatt aaccagctcc tgaatattgc
    19801 tgttccactg cgcgcgcggc aggctgttga tatgcgccag cgccgctctg tcctggtcat
    19861 gaccagagag gtacagcccg taagcgtaaa cctgctccgg gtcgttcggc ttctgctgcg
    19921 ccagattgcg cattaacgta tcggcctggc tgcgttgtcc ggcctgccag agatcctgcg
    19981 aaagtcggta agtaatccat acgctgccgg ggtccagcgc cagtcgttgc cgctgaagtg
    20041 ctgccgcctg cgcccatttg ccctggtttt ccagtgcctc tgcctgctgt gccagacggt
    20101 cgttttgcag gctgcgttcg atatcatcaa tgctacgccg ctgactggca gagagcgagg
    20161 cgataaacgc ttcagctttt tctggcgatt gctggcggta aatatttgcc agcccgcgca
    20221 cggcgttagt gttgccgctg tccatacgca aggtctgctg ataataacgt tcggcggcgg
    20281 gataatcttt tcgcgccatc gccacatcgc ccagccccag cactgcataa ctgtcggtgt
    20341 tatcgacatt acgcgcctgc tggaacaggc gttctgcccg gtcaggatta ttggctttca
    20401 gcgcagcatc gccctgctgg atcgccagcc agtagcggtt tactttcagc agactgttcc
    20461 atttgtcgtt gttgctgctg tgcgggtcca gtgcgagggc tttttccaga ttcgccactg
    20521 cattggcgcg atcgcctttc tgagaatacg cctggcccag cgcccccaga gcttcactgt
    20581 ctttcgggtt cgcccgcacc gcctgttgta gttcgggaat ggctttaccc gccataccag
    20641 agtccaccgc cgctaaacct tgcgcacgag cgcggaaagc aggatcggcc agctgttttt
    20701 gctgttctgc cagttgcgat tgcgcagccg ccacgctatc gccatcacta aagatcgaga
    20761 gatatttttt cagcgccgac acactggcat cactgacggg catgtcttta atctgcccgt
    20821 accagatttt agaggcccct tcgcgcccgg cgttcgattt tgccatctgt tccaggacgg
    20881 caaaaccttc gtcacgccga tcgctactaa acagcaatag cgccagattg ttttgcaggc
    20941 ccgtattgcc cggcgtatcc gcattaatgc gttttagctg attaatcgct tcgccacggc
    21001 gagccggaat tttcgccacc gtactccagt actcgacggc aatgtcacct tccggcggcg
    21061 caccgttgaa cagtttgttg taactcgcca cagcttcttc tgcatgaccg gtcgtcgcct
    21121 gcaatcgtgc ctgttgcagt gcctgacgac catccggcgt ggaaagtagc atcgtagtcc
    21181 gcgacgattt atacgcattt gaactcggcg ctaactgcga cagccgatcg agctgttttt
    21241 gcgcgccatc aatatcgccc tgacgtaaca aagaacggaa acgggcggca acgacgtccg
    21301 ggttattcgg atcaataagt tccagccgat ataacgactg ttgcaccaga tcttcacgat
    21361 gggtcgcttc gcctaaccga acttgctcca gcaactgttg ctgagcggtt ggtgctgcct
    21421 cgaccatcgg catgacggcc agaccgaggg aaagcgtgaa tatgtttagt gtgaatttgc
    21481 gcattcctgg ccccagtcag gtaataactc accttttgtc gagaagcgga aacggtgttg
    21541 atcccagcct tgtccaaaca gggtcagcac atagttgtaa taggcatcgc tgccgggaaa
    21601 gttatcggcc acgcgctggc gctgaacggc ctgcgcatcg cggttttgta aaaagggcag
    21661 catggcggca gaaaaaccga ccggtccttt accctgcgct ttccccgtag ccacatccac
    21721 tttttccggc ggataaccgt ttttctcagt gaatgtcgcc atcggtttaa accggttgag
    21781 catccgcgct ttttgcggat cgctgtcagg catcatgcct acccacatgt aaacgcggat
    21841 agcgtcgtag ctgctgatca atgttttttc ggcttttagc tgccagcctt tgtctttctc
    21901 atagcgcacc cagtctggcg aaaaaccttt cggggcggtt tccagcaata aacgttgatt
    21961 ggtttcgcgc agcgtggtcc acggcgcgcc aaagcgggtg aaatactgcg ccagcgtcgg
    22021 cggcaggtag ctggggttaa aacgccagct gttatcctca gcaaaaccca ctttgcccgg
    22081 taacaacatg gaacccagcc caggcaccgt caccacttcc tcccgcgcga tacgttttag
    22141 caacgcgctg ccgatgtcgg tataacgctg ctctttccac aaacgccccg cctccagcaa
    22201 cgaccaggcc atccagacat caccatcgga ggccgaattg ctgtccagca cttcccactt
    22261 actgttctct ttcttgcccc acagccaggc gggcaaacgt tcttttaaag agccctgagc
    22321 gagattgttc tgcgtccagt cgagaatatt atcgaaagct acacggtcgt tagccgccag
    22381 ggcaaagaac ataccgtaac tttgcccttc ggaggtggtg attttgcgcg cgtcgctggg
    22441 gtcgatgacg cgcccttcct gactgatgta atccttttta aactgctccc aggcaggcca
    22501 ggtacaggct gcctgaacac taaaggcagc cagcagcagc atcgtcacga ttccactacg
    22561 caacacattc atcttcaatt actcgttatc cgggttaaga cgacgacgac taataattcg
    22621 cagcagacgc cacagtaccc atgccagcaa tatcacactg atagccgcca gcaccgccag
    22681 cagaatcgga tggtttgcca gcgcatacca cacgcgctcg aaccacggca gatgacctac
    22741 gtaataaacg tcgccaacac gtaggctgtt gataccggac tcgcggatca ccgcgaccga
    22801 accgaacatg gtggcgcgtt tgccgctatc gttcaccgca tcgttaagca tttcataacc
    22861 gcgtgggcta tctgccaaca gcgcaatcac gctgcgctgg tcgttatacg gcgactggaa
    22921 gccaatcacc gccgccatcg caccggaaga ggtcagcgtt gaccgggttt ctgccgcgcg
    22981 atcgctctcg tccggcacaa tgccggggaa cggggtctgg cgcatcggtg ttttcaccca
    23041 gctttcggtc gcctgcacca ataggtcgat ctgcttatcg tctttcagtt tgtccgggat
    23101 accaccgatg atcatgatgt cggcatcttt gccctgaatg gtgctgccat catcggtcac
    23161 cgtcagatta atcgccggga agcccgtctg tgcgccgata aaaccaacag tattcagcaa
    23221 cgtttccatc tgtgcttcgt taggcgcttt cggcatcacg gtgatggttt gcgacagatc
    23281 cgccatccgg ctgaatggga agcccgcgtt agcaaaggcg cgtagatccg gcatcgggat
    23341 gaagtggtaa tacttcgaga agtcgatggt ggagtcgtca ccaatcacca catgattctg
    23401 caccggctgg aaggtaatac agttatccac cgaaccgccc ggcatcgggt tcatatactc
    23461 aaagtcgaag cgcagctggt tggtcgcgcc cagtttcagc gccggaatag agacatctgt
    23521 tttgccatcc agcaaacctt gtaataccgg aatccgcagc agcaggcggt tcgcctcctg
    23581 tttgctgctc aggttgaagg attgcaggaa ctggttattc aggctgatat ccatccgcga
    23641 actgtctttc accggcggca tggtgtagcg gtaattaata tccatatcaa tgccggtact
    23701 gcgcatcagg tagagatccg gcggcaggtt tagcgaaacg ttaatcgctg ctggctcaag
    23761 accgctggat tgtaactgtt cttcataggt tttcagttcg ccaaatgtga ccggacgatc
    23821 ggtacgtacc cagttcggcg catcgtacgg cttacgcggt agcagcggtt tcacttcatt
    23881 cactaccacg ctttcaccac ggaacagaat gttaccctga gcgatacctt tcgctgcctg
    23941 caacaggtct ttgtcgtcac gaccaaacac caccagcagt ttgacgtaag gattctgcgg
    24001 atggttaatc atctcaatca caggggcttt taccgccgga tgatcgcgca ggaagtccgg
    24061 ccgtttgtcg ttggttgcaa agacaatggc attacgatcc ggcagttggt tatagagcac
    24121 cgggaagttc tgcccacgcc agccagaacg cgaaccaaac cacgaggcga caatggcaga
    24181 ggcttgttgc agcccaacat ccggcgcacc cgcaaagacc atcggcaagg tgttagtacg
    24241 gttatcgcgc gggtcaaaga atggcaccgg gaagtgtgac aggtcattct tcacattcag
    24301 ggtctgatag gtcagatcca gtccactgct ccgcccaaca tccagccaaa gcgtggtgct
    24361 ggccgggttt tcgcacacgt cctgataatg gccgacaaac tccagccgca cacggttgaa
    24421 gtcggtaata aacagtgggt taatgggcat ttgcgccagc gtttttttac ccaactgttc
    24481 tttggtcact ggcagcacgc ccatcagctc atcattgaga taaaccttta actgcgactg
    24541 gacaggcagt aacgatggcg atggcgtgta ttcgaggttg agcatcgcct tcgtcaccac
    24601 ttcatcgctg cgcataccaa actcaatgct gccgttcggg ttaatgccac gtagcaccat
    24661 gctgcccggc ggcggcgcaa tttgcgcaaa ggtcagcttc acatcacgcg aaggaccgtt
    24721 ctgcgccacg actggcgcat cagcgccctg cacgcccggc atcacttgcc ccacctgcgg
    24781 attttgttcc gtctgggcgg ctacagctgg ctcagcattg atcagtggtt gcgttgctgg
    24841 cgtcgcctgc gtcatgaaag aggggaacgc actcatcccc atagccactg cacaaatcca
    24901 gaatagtttt cttttcatcg cgttatcatc attgttgagc caaagcctga tccgatggtt
    24961 gtgccgtttc gctccgctcc gggcggcgcg gaataaacga tacaacccag gaaaccagag
    25021 aagtcagcac acggaatatg cccttcaccg aagaaggcgc aaactccgcc agatggcggt
    25081 agccacggaa gccgagcttc agaatatcca gcagactttc cagcggctta tcttccgggt
    25141 agctgtcctg ccagagcgcc catgtatccg cacgggcaaa cgtacactgc acaaaatcga
    25201 tatgttgctg ggtggtgagc ggcattaatt tcagcccaac ttcattaccc atcacgcgcg
    25261 ccacctgggt cgggaagacg tattcctgct gaccgcgttt aagcaacaga ttcactttct
    25321 gcccttccag aatctgcgcc tgaccgttga tcttgatccc caaaccaccg tcggagaaat
    25381 cctgaacggt acacgagaag aggtgaccat cttcgcgggc aattgccgcg ggcatcgtca
    25441 tctccacgcg gtgcgatcgg cgtacctgtt tgctttctac cgataccgca actgcgccgc
    25501 caagaacaat caggttgtag aacacccaca ccatactgac gaccacggtg agcatctcgg
    25561 ttggcgggcc atagaagtag cgccagatgc ctaccgcaac gcccaccagg ttgagtagaa
    25621 caaggaagat gtagggccgc gagatcaccc agtcgacgta ctcttcttcc accagtccac
    25681 ctttggcggt gacgttaaat ttgcctttgt gcgggttaat cagcgccacc agcgtcggtg
    25741 gtgcgatata ccacgccagc accgtttcgt agatttcact ccagaaagag tggcgatatt
    25801 tgccctggat cttcgagttg gtcaggctgg catggatcat atgcggcagc acgaacaggg
    25861 cgatcatcaa cgctggcgca tagatgatgt aggcatgaag cagcaggaac gccagcggcg
    25921 cagtcaggaa gatcagtcgt ggaatgcccg acaagaagtg gaacatggcg ttgacgtagc
    25981 acagccgctg ggcgaatttc agccctttac cggtaagcgg gttatcgaga cggaagattt
    26041 gcaccatccc gcgtgcccag cgaatacgct gaccgatatg cgccgacaga ctttcggtcg
    26101 ccagccccgc cgcctgcgga atacgcatat atgcagaggt gtagccacga cggtgcaggc
    26161 gcagagacgt gtgcgcatct tcggttaccg tttcgacagc aataccgcca atttcatcca
    26221 atggtttgcg acgaattact gcacaggaac cgcagaagaa agtggcgtcc cacatatcgt
    26281 tgccatcctg caccagacca tagaacagcg tgccttcgtt cggtgtttta cggaaacgcc
    26341 ccaggttgcg ttcaaacggg tccggcgaga agaagtggtg tggagtctgc atcatcgcca
    26401 gctgtttctc tttcaggaac cagcccatgg tcatttgcag gaacgatcgc gttggtacgt
    26461 ggtcgcagtc gaaaatcgac acgaactcgc ctttggcata tttcagcgca ttgttgatgt
    26521 tgcccgcttt cgcatgttca tgagtggtac gggcgatata tttcaccccc acgttttgcg
    26581 caaactggcg aaactcttcc ctaccgccgt catcgaggat ccagatgttc agcttgtctt
    26641 tcggccagtc gatacccagc gaggcgtaaa tagtattttt caccacgttg agatcttcgt
    26701 tgtaagtcgg gacaaagata tccaccgacg gccacagcga catatctttc ggcaatggca
    26761 ccggctgacg attcagcggc catactacct ggaagtagcc gagcaccagc acaatccacg
    26821 cgtacgtttc agcgaagagc agaataagcc cgcacaccag gctgaccgga tcgtcccagt
    26881 tcagcgtaga ggtgtaacgc caccagatat aacggcaaga aacggtcagc gacagcacaa
    26941 tcaacattag cgccgagaag cgccccggca tccgccgtac gatcagcgct acaccccaca
    27001 gcagcatcag gaagataaac tgcgccagcg ggttaaacgg ttgagtaacg cagattaacg
    27061 ccagaatcag cgagaaggtg acgatgatac cgaggatcaa ccgccgcgcc ccggcactca
    27121 aatgaccgag ctcttttttc tcatcaagat gctgtgtttt atggctaacg cgctcaggca
    27181 gctcgttcat ccattgatgg taacgtccac gaatattctg cagacctgaa aatgcccgcc
    27241 tgcgcggttt cggcgtttct ttgcgcgatg taccgatcag taaccagcat gtttgaatga
    27301 gataacggac cgggtccagc ggacgcggac gcgaggcgtt gatatgcgga tacaggtttt
    27361 tatgttctgc gcgaatacgc tgccagcgcg ggtgctccag cggaataaaa atccaggcca
    27421 ggatcatcca gaaacagccg agcgtcgcgc tgaaagccga cgcaccgtga cgacgataat
    27481 cgcgataacg cccgataagc cgcgcgttga ccggcgggat aagcaaccac cgggtcagga
    27541 tactcatgat gcactcccga ctggcgtttt cagcccggag tagttcaaca ggcaccagtt
    27601 cgccagcgtc agtatctctt cagccgccag cgcatcactg cgatattcac ctactggttg
    27661 cttagccgcc aggcattcag ccatcgcttc atcacgatga atgagcatcg gcagtaatcg
    27721 gcgctggctt tgcaaccaaa gctggtaaat atcgtcctga acctgactgc caatacggaa
    27781 gtcattaatc aaaatatgtg cgccatccgg cagcgcttgc tgatgcagtc ggatatggca
    27841 gttggcatcc acattgacga ttgccagcga gtgatcgcac aaactcagca gctggtgggt
    27901 tatctgcgag gcatcacgcg gtaagtcgat taaaatccac tggtaacgcc cgctggcttt
    27961 tagttgctgt aagccggagc aaatatcgct cagccgggtt tgccagtgct gtggattttc
    28021 ttgttcttca atggataact gaccaaaagg cagcaaatcg agctgcgagg tgtagtgcaa
    28081 cccagcgtca cgccagtcct ggccatccag catcgctctg gcccagccct gacggtgggt
    28141 aaaatcaaca ttaaatgaca ggcgcaacaa gttgcccggg caggcatcga ccaccaggac
    28201 attttctccc aacatttgta atgaccaggc taatgcggcg gtgatggttg ttgtccccac
    28261 gcctccccgc accccctgca atcccagtac ggccattcac caatccccta cttttgttgc
    28321 gcaaactctg ccagcaacgg ccagcgtttt aatgccgcgg ccaactgttc gcgttgggaa
    28381 atatcggcat aatcaatatc aggcagtgaa aatgcctgct ttaacgccac aatatcattc
    28441 tggaagatat agcctatcgc gggatcaggc agagtatctg gttcattgtt attcattttg
    28501 cttgatccct atgaaataac cgtaccgttc atgagacaat tctaaccgtg agcatttcgc
    28561 cggtttattg tttacggcga gacgggaatt tgctcacttt ttgactttcc gttacgcaac
    28621 agaaaaagtt aaaaacgaaa agactaaaat cacacgtctt gtgaccttct aaattgattc
    28681 acatgctaaa tctgataagt tttaatttca atggtaggtt tatttcttag ctttcgctag
    28741 taaactgata atcagacaaa atggtgacat gagggacatt gtggaccctg tattctctat
    28801 cggtatctca tcattatggg atgagctgcg acatatgcca gcaggcggcg tctggtggtt
    28861 taacgtcgat cgccatgaag atgctatcag tctggcgaat caaacaattg catcccaggc
    28921 tgaaaccgca cacgtcgcgg tcattagcat ggacagcgat ccggcgaaaa tctttcaatt
    28981 agatgattct caagggccgg aaaaaataaa attattttca atgctaaatc atgaaaaagg
    29041 tctatactat ttgacccgtg atttgcagtg ttctattgat ccccataatt acctttttat
    29101 tcttgtttgc gcaaataacg catggcaaaa cattcctgcc gagcggcttc gctcatggtt
    29161 ggataaaatg aataaatgga gcaggttaaa ccattgttcg cttttggtaa ttaatcccgg
    29221 aaataataac gataaacaat tttcattgtt gcttgaggaa taccgttcac tttttggtct
    29281 tgccagtttg cgttttcagg gtgaccaaca tttgctggat attgccttct ggtgcaacga
    29341 aaaaggggtc agcgcccgtc agcagcttag cgttcagcaa caaaatggta tctggacatt
    29401 agttcaaagc gaagaggcgg agatccaacc acgcagcgac gaaaaacgca ttctgagtaa
    29461 tgttgctgta ctggaaggtg cgccgccgct atcggaacac tggcaactgt tcaacaataa
    29521 cgaagtcctg ttcaatgaag cccgtaccgc tcaggcggcg acggtggtct tttctttaca
    29581 gcaaaatgcg caaatcgagc cactggccca cagcattcat accctgcgtc gccagcgcgg
    29641 tagtgcgatg aaaatcctcg tgcgggaaaa taccgctagc ctgcgcgcca ccgatgaacg
    29701 tttgttattg gcctgcggtg caaatatggt tattccgtgg aatgcgccac tctcccgttg
    29761 tctgacgatg atcgaaagcg tgcaagggca gaagtttagt cgctatgtgc cggaagatat
    29821 cactaccttg ctgtcaatga cccagccgct caaactgcgt ggtttccaga agtgggatgt
    29881 gttctgtaat gccgtcaaca acatgatgaa taaccctctc ttgcctgccc acggtaaagg
    29941 cgttctggtt gccctacgtc cggtaccggg tatccgcgtt gaacaagccc tgacgctgtg
    30001 tcgccctaac cgtaccggcg atatcatgac cattggcggt aatcggctgg tgctgtttct
    30061 ctcattctgt cggattaacg atctggatac cgcgttgaat catattttcc cattgcctac
    30121 tggcgacatt ttctcaaacc gtatggtctg gtttgaagat gatcaaatca gtgccgagct
    30181 ggtgcagatg cgcttgcttg ccccagaaca atggggcatg ccgctgcctt taacgcaaag
    30241 ttctaaaccg gtcatcaatg ccgagcacga tggtcgccac tggcgacgaa taccagaacc
    30301 catgcgactg ttagatgatg ctgtggagcg ctcatcatga tgaccatcag cgatatcatt
    30361 gaaattattg tcgtttgcgc actgatattt ttcccgctgg gctatctggc gcggcactct
    30421 ttgcgacgca ttcgcgacac cttacgtttg ttctttgcta aacctcgtta tgttaaaccg
    30481 gccgggacgt tacgccgcac ggaaaaagcc agggcaacca aaaaatgact caatttacgc
    30541 aaaataccgc catgccttct tccctctggc aatactggcg cggcctttcc ggctggaact
    30601 tctattttct ggttaagttc ggcctgttgt gggcgggata tcttaacttc catccgctcc
    30661 tcaatttggt gtttgccgcg tttctgctga tgcccattcc gcgctacagc ctgcatcgct
    30721 tgcgccactg gattgccctg ccgatcggct ttgctttgtt ctggcatgac acctggttgc
    30781 ctggcccgga aagcataatg agccagggtt cgcaggtggc ggggttcagt accgattatt
    30841 taatcgacct tgtcacacgc tttattaact ggcagatgat tggggccatt tttgttttat
    30901 tagtggcctg gttattcctg tcacaatgga ttcgcattac cgtttttgtg gttgccatac
    30961 tgctatggct gaacgtactt accctggcgg gaccaagttt ctccttgtgg ccagccggac
    31021 aaccgacgac cactgtaaca acgacgggtg gtaacgcagc ggcaaccgtt gcggcgacgg
    31081 gtggcgcacc ggtagtgggt gatatgcccg cacaaactgc accgccaaca acggcgaacc
    31141 ttaacgcctg gctgaataat ttctataacg cggaggcgaa acgtaaatcg accttcccgt
    31201 cttcgctgcc cgctgatgct cagccatttg aactactggt gattaacatc tgttcgcttt
    31261 cctggtcgga tatagaagcc gccgggttga tgtcgcatcc actgtggtcg catttcgata
    31321 ttgagttcaa gaactttaac tccgccacct cctacagtgg cccggcggcg atccgtttac
    31381 tgcgcgccag ctgcgggcag acttcgcaca ctaatctgta tcaaccggca aataacgact
    31441 gctatctgtt tgataacctt tcgaaactgg gctttaccca gcacctgatg atggggcata
    31501 acggccagtt cggcggtttt ttgaaagaag ttcgcgaaaa tggcggcatg cagactgaat
    31561 tgatggatca aacaaatctg ccggttattt tgctgggctt tgatggttcg ccggtttatg
    31621 acgataccgc cgtgcttaac cgctggctgg acgttaccga aaaagataaa aatagccgta
    31681 gtgccacgtt ctacaacacg cttccactgc atgacggcaa ccattatccg ggggtcagca
    31741 aaacagcgga ttacaaagcg cgggcgcaga aattctttga tgaactggac gccttcttta
    31801 ctgaactgga gaaatcgggt cgtaaagtga tggtggtcgt ggtgccggaa cacggcggcg
    31861 cgctgaaggg cgacagaatg caggtatctg gcctacgtga tatccctagc ccgtctatca
    31921 cagacgtccc cgttggggtg aaattcttcg gcatgaaggc accacatcag ggggcaccga
    31981 ttgtcattga ccaaccgagc agcttcctgg ctatctccga tctggtggtt cgcgttcttg
    32041 atggcaagat tttcaccgaa gacaatgttg actggaaaaa actcaccagt gggttgccac
    32101 aaacagcacc ggtctccgag aactcaaatg cagtagttat tcaataccag gataaaccgt
    32161 acgttcgcct gaacggcggc gactgggtgc cttacccgca gtaagcacga aaaaggccgc
    32221 agagtttaac ctctgcggcc tgatccgggc gcaaattgcc attacggcag cctgacgccc
    32281 gcatgacacg ttacttccgc tt
//
LOCUS       DAFNBJ010000041        32135 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000041 DAFNBJ010000000
VERSION     DAFNBJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.041"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(113..2182)
                     /gene="glyS"
                     /locus_tag="L2X49_001787"
     CDS             complement(113..2182)
                     /gene="glyS"
                     /locus_tag="L2X49_001787"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBN4796882.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2192..3103)
                     /gene="glyQ"
                     /locus_tag="L2X49_001788"
     CDS             complement(2192..3103)
                     /gene="glyQ"
                     /locus_tag="L2X49_001788"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBN4796883.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3198..3497)
                     /locus_tag="L2X49_001789"
     CDS             complement(3198..3497)
                     /locus_tag="L2X49_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBN4796884.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3672..4667
                     /gene="wecH"
                     /locus_tag="L2X49_001790"
     CDS             3672..4667
                     /gene="wecH"
                     /locus_tag="L2X49_001790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBN4796885.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4709..5146)
                     /locus_tag="L2X49_001791"
     CDS             complement(4709..5146)
                     /locus_tag="L2X49_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBN4796886.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5192..5533)
                     /locus_tag="L2X49_001792"
     CDS             complement(5192..5533)
                     /locus_tag="L2X49_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796887.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5702..7156)
                     /gene="xylB"
                     /locus_tag="L2X49_001793"
     CDS             complement(5702..7156)
                     /gene="xylB"
                     /locus_tag="L2X49_001793"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBN4796888.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEERVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCYALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7228..8550)
                     /gene="xylA"
                     /locus_tag="L2X49_001794"
     CDS             complement(7228..8550)
                     /gene="xylA"
                     /locus_tag="L2X49_001794"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBN4796889.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8916..9908
                     /gene="xylF"
                     /locus_tag="L2X49_001795"
     CDS             8916..9908
                     /gene="xylF"
                     /locus_tag="L2X49_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN4796890.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9986..11527
                     /gene="xylG"
                     /locus_tag="L2X49_001796"
     CDS             9986..11527
                     /gene="xylG"
                     /locus_tag="L2X49_001796"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBN4796891.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11505..12686
                     /gene="xylH"
                     /locus_tag="L2X49_001797"
     CDS             11505..12686
                     /gene="xylH"
                     /locus_tag="L2X49_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBN4796892.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12764..13942
                     /gene="xylR"
                     /locus_tag="L2X49_001798"
     CDS             12764..13942
                     /gene="xylR"
                     /locus_tag="L2X49_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HBN4796893.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14050..14874)
                     /locus_tag="L2X49_001799"
     CDS             complement(14050..14874)
                     /locus_tag="L2X49_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBN4796894.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15194..17224
                     /gene="malS"
                     /locus_tag="L2X49_001800"
     CDS             15194..17224
                     /gene="malS"
                     /locus_tag="L2X49_001800"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBN4796895.1"
                     /translation="MKLAACFLTLLPGFAVATSWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMT
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17402..18655
                     /gene="avtA"
                     /locus_tag="L2X49_001801"
     CDS             17402..18655
                     /gene="avtA"
                     /locus_tag="L2X49_001801"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBN4796896.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18806..19279)
                     /locus_tag="L2X49_001802"
     CDS             complement(18806..19279)
                     /locus_tag="L2X49_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBN4796897.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVNAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19381..20229)
                     /gene="yiaJ"
                     /locus_tag="L2X49_001803"
     CDS             complement(19381..20229)
                     /gene="yiaJ"
                     /locus_tag="L2X49_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBN4796898.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20430..21428
                     /gene="yiaK"
                     /locus_tag="L2X49_001804"
     CDS             20430..21428
                     /gene="yiaK"
                     /locus_tag="L2X49_001804"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBN4796899.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21440..21907
                     /locus_tag="L2X49_001805"
     CDS             21440..21907
                     /locus_tag="L2X49_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBN4796900.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            22025..22498
                     /gene="yiaM"
                     /locus_tag="L2X49_001806"
     CDS             22025..22498
                     /gene="yiaM"
                     /locus_tag="L2X49_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HBN4796901.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22501..23778
                     /gene="yiaN"
                     /locus_tag="L2X49_001807"
     CDS             22501..23778
                     /gene="yiaN"
                     /locus_tag="L2X49_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HBN4796902.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAK
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            23791..24777
                     /gene="yiaO"
                     /locus_tag="L2X49_001808"
     CDS             23791..24777
                     /gene="yiaO"
                     /locus_tag="L2X49_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HBN4796903.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            24781..26277
                     /gene="lyxK"
                     /locus_tag="L2X49_001809"
     CDS             24781..26277
                     /gene="lyxK"
                     /locus_tag="L2X49_001809"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HBN4796904.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            26274..26936
                     /gene="ulaD"
                     /locus_tag="L2X49_001810"
     CDS             26274..26936
                     /gene="ulaD"
                     /locus_tag="L2X49_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBN4796905.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            26929..27789
                     /locus_tag="L2X49_001811"
     CDS             26929..27789
                     /locus_tag="L2X49_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBN4796906.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27783..28478
                     /gene="araD"
                     /locus_tag="L2X49_001812"
     CDS             27783..28478
                     /gene="araD"
                     /locus_tag="L2X49_001812"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBN4796907.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28510..28611)
                     /locus_tag="L2X49_001813"
                     /pseudo
     CDS             complement(28510..28611)
                     /locus_tag="L2X49_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            28615..28803
                     /locus_tag="L2X49_001814"
                     /pseudo
     CDS             28615..28803
                     /locus_tag="L2X49_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28825..29565)
                     /locus_tag="L2X49_001815"
     CDS             complement(28825..29565)
                     /locus_tag="L2X49_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HBN4796908.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNKNT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            29689..30663
                     /locus_tag="L2X49_001816"
     CDS             29689..30663
                     /locus_tag="L2X49_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4796909.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(30660..31796)
                     /locus_tag="L2X49_001817"
     CDS             complement(30660..31796)
                     /locus_tag="L2X49_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBN4796910.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(31802..32125)
                     /locus_tag="L2X49_001818"
     CDS             complement(31802..32125)
                     /locus_tag="L2X49_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBN4796911.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
BASE COUNT         7987 a         8238 c         8016 g         7894 t
ORIGIN      
        1 aattaatata tgagataatc tttattcagc agaagattat taaaggttgc tgtattattt
       61 agcgataata aaaaaagcct gccagatggc aggcttttta ataacggcgt tattactgca
      121 acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag tttctccagc atggtcagac
      181 ggttgagacg caattctttg tcatcaacca tgaccatcac tttatcgaag aaagcatcaa
      241 ccggctcacg cagctctgcc agttcgacca gcgcatcctg gtaacgacct tccgcaaagt
      301 acggctccag cttgtcacgt agcaccacaa cctgcatcgc cagtttaatt tcttccggct
      361 ctttcagggt agaggcattc acgcggtcgc tcagcacttc gtcagatttc gccaaaatgt
      421 tggagacacg cttgttcgcc gccgccagtg cagcagctgc ttcaagggta cggaagtgcg
      481 ataccgcttt catgcgggca tcgaaatcag ccggacgagt cggacgacgc gccagtaccg
      541 cctggatggt gtcaacggtg taaccttcgt cctgatacca ggcgcggaag cgaccgagca
      601 taaagtcgat aacatcatca accacgttgg cgttagtcag cttatcgcca tacagacgca
      661 ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt cttctcaacg ataattcgca
      721 gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt gtcgcctttc ggatgctggc
      781 cgataccgaa gatacccgcc agagtatcca tcttgtcagc aatcgccagc gcacaggcta
      841 ccgggttaga cggcaggtca tcaccggcaa agcgcggctg atactgctca ttcagcgcca
      901 ccgcgacatc ttccgcttcg ccatcgtgac gcgcatagtg catccccata acgccctggg
      961 tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca cttagacagc aggcccgcac
     1021 gggttgcgtg gttaacgtca gcgccaatct gttcagcaat ccagccagcc agcgcctgga
     1081 tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg gaacagcacg gtttgcaggc
     1141 gcggcaggtt atcttccaga cgttttttac ggtcggtgtt gaagaagaac tcggcatccg
     1201 ccagacgcgg acgaacgact ttctcgttac cggagataat ctgctgcgga tctttcgatt
     1261 cgatgttggc aacgaagata aagttcggca gcagtttgcc gtcgttcgca tacaccggga
     1321 agtatttctg gtcacctttc atggtgtaaa ccagcgcttc agccggcacc gcgaggaatt
     1381 tctcttcgaa ttttgcggtc agaacgaccg gccactccac cagcgaagcc acttcttcca
     1441 gcaggctttc gcttaagtca gcgttaccgc caatcttacg cgctgcttct tcggcatcgg
     1501 ctttaatctt cgctttacgt tcttcgtaat cggcgatgac tttcccgcgc tcacgcagaa
     1561 tttccggata ctgatcggcg ttgtcgatgg tgaattccgg ttcgcccata aagcggtggc
     1621 cgcgaatcac gcgatcggac tgaatgccca gaatggttgc cggaatgact ttgtcgccca
     1681 gcagcagggt cacggtgtgc accggacgca cgaagtgcac gtcgcttgcg ccccaacgca
     1741 tcagtttcgg gatcggcagt ttcgccagag aagtcgcaac catattcggc agcagtgctt
     1801 cggtgctttc gcccttcaca tgggcgcgat acagcagcca ttcgccttta tcggtagtca
     1861 gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc ccaaccttct gccgctttgc
     1921 tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg gccgcgtttt tcgatttcac
     1981 gatccggttg cgcttccgcc aggttagcta ctttcagcgc cagacgacgc ggagcagcaa
     2041 accattgaac ggtgccgtgt gcgaggccag cgttatccag ctccgcagta aaattcgcag
     2101 caaaggactc agccaggctg cgcagtgctt ttggtggcag ctcttcagtg ccgatttcca
     2161 ccagaaaagt tttctcagac atagccgcct cttacttatc tttgttgcac atcgggaagc
     2221 cgagggcttc acgggaagcg tagtatgctt ctgccactgc tttggtcagg gtgcgaatgc
     2281 gcagaatgta gcgctgacgc tcggtgacgg agatggcttt acgcgcatcc agcaggttga
     2341 agctgtgggc ggctttcaga atacgctcgt aggctggcag cggcagcgga ttttccagcg
     2401 ccagcagctg ctgcgcttct ttctcgtact gctcaaagca ggtgaacagg aagtccacat
     2461 ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt ctgatggaac acgtcgccgt
     2521 aggtggtttt acccagcggg ccgtcgctcc agaccaggtc gtaaacgctg tctacgccct
     2581 gaatgtacat ggccagacgt tccagaccgt aggtgatctc gccggtaacc ggtttacact
     2641 ccagaccacc aacctgctgg aagtaagtga actgcgtcac ttccatgccg tttagccaca
     2701 cttcccagcc cagtccccag gcacccagag tcgggttttc ccagttatct tccacgaaac
     2761 ggatgtcgtg aatagtcggg tccatgccca gctctttcag agaaccgagg tacagctcct
     2821 gaatattgtc cggcgatggc ttaatgacca cctggaactg atagtagtgc tgtaaacggt
     2881 tggggttttc gccgtagcga ccatcggtcg ggcgacgaga aggctgaaca taagcagccg
     2941 ccatcggttc tggccccagc gcgcgcagac aggtcattgg gtgagaggtt cccgcgccga
     3001 cttccatgtc caatggttga acaatggtgc agccctggcg agcccagtaa tcctgtaagg
     3061 tcaggatcaa gccctggaag gtcctggtat caaacttttg catattattt cgtgctggat
     3121 acgtgtggat ttaaaggaag ggatcagtat acccgctgga tggaagatat acagtacgaa
     3181 acgggaaaaa gcagggctta acgcatggaa agatgcaaaa aatggccatc tgcgtcaaaa
     3241 gaacagacaa actgctcttt acggaaggta taaccgcgca tttcatagct tccctggaac
     3301 tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt tataacgttt tgccgcctga
     3361 tccttgcata acgcttccat attcaacgaa cgcagaggat caacctttac ccgttgtgct
     3421 ttctgcacgg gtgacggtgt agaacaaccc actagcacca taagcgccat tgccggaaag
     3481 aaagcgttca tcatcattaa attaccgtct ggtttgcagg tgagtcttat tatttatatc
     3541 ggtatagtgg ctgatatctt acgaatttcc ttaagcctct ataagctgta gtgataaaac
     3601 tcagattatt ccagtgcgcc aactgaatga tttatcggtc agactttttt taaccagata
     3661 cagagacaca gatgcagccc aaaatttact ggattgataa cctgcgaggg atagcgtgtt
     3721 taatggtggt gatgattcac accactacct ggtatgtgac caatgctcat agtgttagcc
     3781 ccgtcacctg ggatatcgcc aatgttctga attctgcctc tcgtgtcagc gtgccgctat
     3841 ttttcatgat atccggctat ctcttttttg gcgaacgcag cgcccagccg cgccatttct
     3901 tgcgtatcgg cttatgcctg attttttata gcgcaatcgc actgctctac attgcgctgt
     3961 ttacctccat caatatggag ttagcgctga aaaacctgct gcaaaagcca gtgttttacc
     4021 acttgtggtt tttcttcgcg attgcggtga tttatctggt ttcaccgctg attcaggtga
     4081 agaacgtcgg cggaaaaatg ttgctggtgc taatggtggt gattggcatt atcgctaacc
     4141 caaacacagt gccgcagaaa attgacggtt ttgaatggct gccaattaac ttatatatca
     4201 atggcgatac tttttactac attctgtatg gcatgttggg ccgcgctata gggatgatgg
     4261 acacacagca taaagcactg tcgtgggtga gcgccgcgct gtttgcgacg ggggttttta
     4321 ttatctctcg cgggacatta tatgaattgc agtggcgcgg aaattttgcc gatacctggt
     4381 atctttactg tgggccgatg gtttttatct gcgcaatcgc gctattgact ctggttaaaa
     4441 acacgctgga tacgcgtacc attcgcggac ttggcttaat ctcccgccat tcattgggta
     4501 tatacggatt ccacgccttg attatccatg cgctgcgcac ccggggaatt gagcttaaaa
     4561 attggccaat actggatatt atttggatct tttgcgcgac gttggcagcg agtttgttac
     4621 tttctatgct ggtacaacga atcgacagaa acagattagt gagttaagta aaagcccgtt
     4681 cacattggac tgaccgggct tacgtgaatt attcgctgta ttcttcctgg gtaacctgtg
     4741 tttcttttgg cgggtttatt ccggcatcac gaatattctt ctgcaccgct actgcaccaa
     4801 acaagcttaa gaagaaagcc agtccataaa aacctttttc actgagtaat aatgtcgcat
     4861 tccacagacc taccatcagt aatgcaacag agataataaa gacagtcagg caggtcatat
     4921 aataaatgga agtggtcggt atgccttcat acttgtcccg aacggtcttt tgataagacg
     4981 cagcagaaaa cagtcctaac accagtacgg caaaataata ccctttttca tttaactgca
     5041 tctctgcatt ccatagccct aacagatagg taacgatacc accaacgaga gctatccatg
     5101 acacaatact aaaggccggt gaataggttg atattttgtt gtccatcaca gtattccttt
     5161 cattcctgaa tgtgtaagag ctttatgttg ctcataccga gcgaaacaaa cgcatttgac
     5221 caaacaagca gacaaaaaag gcgatggggt agatcaccat ttcataagca ttgataggtg
     5281 catttaaaac accgataaac aagagaccga tagtgattaa cgcaactaac tggcagatat
     5341 gggtaaaaaa attatccagt tgcccgagtt tttctgcgcg aagatatgca tagttaccaa
     5401 aagttgctgt cattaacacg ccaagaaaat agccttttcc acttagcaat gggcatgaga
     5461 tccatagccc aaccagataa accagcgccc cgacaacaaa taatagtttt gccacagttt
     5521 ttgaggtttt cataacgatc tccatatcta ccagcgatac attacgagta accaacgaaa
     5581 gacaaaactg aaaaatgcca ttaacaaatg attttcagaa taaattcata ctaaatatta
     5641 attaattagt gagatatata gatgtgaatt atcccccacc cggtcaggca ggggataacg
     5701 tttacgccat taatggcaga agttgctgat agaggcgacg gaacgtttca cgtcgtggct
     5761 gataagcggc ataacgctgc gcatctggta gatgcgactg ttctaacggt agctgcggca
     5821 acaactcaat gagcgatttc tctggattcg ccgcaatctg cgccagcctt gctgcgccca
     5881 gtgctggccc cacatccccc cccgtacggt aatcgagctg ctgaccgctg atatccgcca
     5941 gcatctgacg ccagtactca ctacgcgccc cgccaccaat caacgtaaca ctttgcggtt
     6001 taatgccgca ggcatgcacg acatccatgc catctgccag cgcataaccc acgccttcca
     6061 gcactgctcg cgccagttca ttggggccat gttgatgagt caaaccaaag aaaaccccct
     6121 tcgcctgggg attattgtgt ggtgtacgct cgccggaaag ataaggcaga aaccaaactg
     6181 gctcggcact ttcatcagcc tgttgagctg cagcgattaa agctgggaca ttgcacaggc
     6241 cggttaattt cgcgacccaa tccagacacg atgctgcact cagcattaca gacattaaat
     6301 gccaacgctg cggtagcgca tagcaaaagc tgtgtacagc gctttctggc ttgcttaaga
     6361 acccttcgct gacagcaaaa tagacccccg acgtccccag cgataacatt gcctgattag
     6421 catcaaccat tcccacacca actgcaccag ctgcattgtc gccaccgcct gcgacaactg
     6481 gcaccgtcgc cataccccac gctttcgcaa cttcaggtaa caaagcacca gtaatttcgc
     6541 tgccttcgta taatgcgggc atctggtcac gagataagtc gcaagcctgc agcatgacgt
     6601 cactccagtc acgctttgcg acatccagcc acatggtgcc agctgcgtca gacatatcgc
     6661 tggcaaactc ccccgtcata cgcagacgca agtaatcttt cggtaataat actttgtcga
     6721 tttgacggaa tatctccggc tcatgccgct gaacccatag caatttaggc gcagtaaatc
     6781 cgggcatcat caggttgccg gtaatcaccc gcgattgcgg aactcgctct tccagcaaag
     6841 cgcactcttg cgcacagcgc ccgtcgttcc acaaaatggc agggcgcaat acccgttgtt
     6901 gagcatccag taaggttgca ccgtgcatct ggccggcaat acccaatgct ttaacgtctt
     6961 gcaaagaatg ctgaacgccc agagctttca ttgcgcgatc agttgcctgc caccactgtt
     7021 ccgggtcttg ttccgaccag agtggatgcg ggcgcgaaac ggtcagcttt tccgtttgcg
     7081 aagcaaccac ctcaccctgc tcgttgagca aaataacttt tacgcccgag gtgccaagat
     7141 ctatcccgat atacatatcg atcgttcctt aaaaaaatgc ccggtatcgc taccgataac
     7201 cgggccaacg gactgcacag ttagccgtta tttgtcgaac agataatgat ttaccagatt
     7261 ttccagttgc tcctggcgac cactctgatg caccggagac aaattatgtt cctgagcata
     7321 tttggctaaa tctgccagtg acatttggcc tttcaggatt tgctggccca attcgctatt
     7381 ccagccggaa taacgctgcg cgatgcgttt atccagctcg ccatcttcaa tcatgcgcgc
     7441 tgcaattttc agcgccagtg ccatcgtatc catcgcgccg atatgaccgt aaaacagatc
     7501 atatttatca gtactttgac gacgtacttt ggcatcgaag ttcaaaccac cggtggtgaa
     7561 accgcctgct ttgagaattt catacatcac cagcgcattc tcttccacac tgttcgggaa
     7621 ctggtcggtg tcccagccca gttgcgcatc gccacggttg gcgtcgacag aaccgaacag
     7681 gccaagcgca atggcggtgg ctatttcatg atggaaagag tgacctgcca gcgtcgcgtg
     7741 gttagcttca atgttcagtt taatctcttt ttccagacca aactgtttca ggaagccata
     7801 gaccgtcgcg gcatcgtaat catattgatg tttagtcggt tcttgcggtt tcggttcgat
     7861 aagcaacgtg ccctggaagc cgattttatg tttatgctca accaccatct gcataaagcg
     7921 gcccagttgt tcacgctcct gacgcaagtc ggtatttaac agcgtttcgt aaccttcacg
     7981 accaccccac aggacatagt tttcaccgcc caatttatgg gttgcttcca tcgctgtaac
     8041 aacttgcgtt gccgcccagc tgaagacttc aggatctggg ttcgtcgccg cacccgcgcc
     8101 atagcgaggg tttgtaaagc agttagcggt tccccacagc agcttcacgc cgctctcttc
     8161 ttgcttggct gccaggacat caaccatttg cgcaaaatta ttgatgtact cttttaacga
     8221 cgcgccctca ggggaaacat ccacatcgtg gaagcaataa aatggcacat gtaacttgtg
     8281 gaaaaactca aatgcgacat ctgctttacg cttcgccaac gccagtgcct caccaggctg
     8341 ctgccacgga cgattaaacg cccccacacc aaacatgtcc gccccgttcc agcagaaggt
     8401 gtgccagtag caggcggcaa aacgcaagtg ctcttccata cgcttaccca acaccagttc
     8461 gtcgggattg tagtgacgga atgctaacgg gtttgaggat tttgagcctt cataacgaac
     8521 gcgatcgagc tggtcaaaat aggcttgcat attgaactcc ataatcaggt aatgccgcgg
     8581 gtgatggatg atgtcgtaat attgggcact ccctttcagt tgctcaatta tgttatttca
     8641 cactgctatt gagataattc acaagtgtgc gctcgctcgc aaaataaaat gggatgatga
     8701 aactgggtaa ttcctcgaag ataaaaatgc aataagtaca attgcgcaac aaaagtaaga
     8761 tctcggtcat aaatcaagaa ataaaccaaa aatcgtaatc gaaagataaa aatctgtaat
     8821 tgttttcccc tgtttagttg ctaaaaattg gttacgttta tcgcggtgat tgttacttat
     8881 taaagctgtc ctctaactac agaaggccct acaccatgaa aataaagaac attctactca
     8941 ccctttgcac ctcacttctg cttaccaacg ttgcggcaca cgccaaagaa gtcaaaatag
     9001 gtatggcgat tgatgatctc cgtcttgaac gctggcaaaa agatcgagat atttttgtga
     9061 aaaaggcaga atctctcggc gcgaaagtat ttgtacagtc tgcaaatggc aatgaagaaa
     9121 cacaaatgtc gcagattgaa aacatgatta accggggtgt cgatgttctt gtcattattc
     9181 cgtataacgg tcaggtatta agtaacgttg taaaagaagc caaacaagaa ggcattaaag
     9241 tactagctta cgaccgtatg attaacgatg cggatatcga tttttatatt tctttcgata
     9301 acgaaaaagt cggtgaactg caggcaaaag ccctggtcga tattgttccg caaggtaatt
     9361 acttcctgat gggcggctcg ccggtagata acaacgccaa gctgttccgc gccggacaaa
     9421 tgaaagtgtt aaaaccttac gttgattccg gaaaaattaa agtcgttggt gaccaatggg
     9481 ttgatggctg gttaccggaa aacgcattga aaattatgga aaacgcgcta accgccaata
     9541 ataacaaaat tgatgctgta gttgcctcaa acgatgccac cgcaggtggg gcaattcagg
     9601 cattaagcgc gcaaggttta tcagggaaag tagcaatttc cggccaggat gcggatctcg
     9661 caggtattaa acgtattgct gccggtacgc aaactatgac ggtgtataaa cctattacat
     9721 tgctggcaaa tactgccgca gaaattgccg ttgagttggg caatggtcag gaaccaaaag
     9781 cagataccac actgaataat ggcctgaaag atgtgccctc ccgcctcctg acaccgatcg
     9841 atgtgaataa aaacaacatc aaagacacgg tagttaaaga cggattccac aaagagagcg
     9901 agctgtaagc gtcacgcccc ggcgcggagc gggggcgtga tttctctcca tgccgcgtga
     9961 atgaattggc ttaggtggag tcgttatgcc ttatctactt gaaatgaaga acattaccaa
    10021 aaccttcggc agtgtgaagg cgattgataa cgtctgcttg cggttgaatg ctggcgaaat
    10081 cgtctcactt tgtggggaaa atgggtctgg taaatcaacg ctgatgaaag tgctgtgtgg
    10141 tatttatccc catggctcct acgaaggcga aattattttt gcgggagaag agattcaggc
    10201 gagtcacatc cgcgataccg aacgcaaagg tatcgccatc attcatcagg aattggccct
    10261 ggtgaaagaa ttgaccgtgc tggaaaatat cttcctgggt aacgaaataa cccacaatgg
    10321 cattatggat tatgacctga tgacgctacg ctgtcagaag ctgctcgcac aggtcagttt
    10381 atccatttca cctgataccc gcgttggcga tttagggctt gggcaacaac aactggttga
    10441 aattgccaag gcacttaata aacaggtgcg cttgttaatt ctcgatgaac cgacagcctc
    10501 attaactgag caggaaacgt cggttttact ggatattatt cgcgatctac aacagcacgg
    10561 tatcgcctgt atttatattt cgcacaaact caacgaagtc aaagcgattt ccgatacgat
    10621 ttgcgttatt cgcgacggac agcacattgg tacgcgtgat gctgccggaa tgagtgaaga
    10681 cgatattatc accatgatgg tcgggcgaga gttaaccgcg ctttacccta atgaaccaca
    10741 taccaccgga gatgaaatat tacgtattga acatctgacg gcatggcatc cggttaatcg
    10801 tcatattaaa agagttaatg atgtctcgtt ttccctgaaa cgtggcgaaa tactgggtat
    10861 tgccggactc gttggtgccg gacgtaccga gaccattcag tgcctgtttg gcgtgtggcc
    10921 cggacaatgg gaaggaaaaa tttatattga tggcaaacag gtagatattc gtaactgtca
    10981 gcaagccatc gcccagggga ttgcgatggt acccgaagac agaaagcgcg acggcatcgt
    11041 tccggtaatg gcggttggta aaaatattac cctcgccgca ctcaataaat ttaccggtgg
    11101 cattagccag cttgatgacg cggcagagca aaaatgtatt ctggaatcaa ttcagcaact
    11161 caaagttaaa acgtcgtccc ccgaccttgc tattggacgt ttgagcggcg gcaatcagca
    11221 aaaagcgatc ctcgctcgct gtctgttact taacccgcgc atcctcattc ttgatgaacc
    11281 tactaggggt atcgatattg gcgcgaaata cgagatctac aaattaatta accaactcgt
    11341 ccaacagggt attgccgtta ttgtcatctc ttccgaatta cctgaagtgc tcggccttag
    11401 cgatcgggta ctggtgatgc atgaagggaa actaaaagcc aacctgataa atcataacct
    11461 gactcaggag caggtgatgg aagccgcatt gaggagcgaa catcatgtcg aaaagcaatc
    11521 cgtctgaagt gaaattggcc gtaccgacat ccggtggttt ttctgggctg aagtcactga
    11581 atttgcaggt cttcgtgatg attgcagcta tcatcgcaat catgctgttc tttacctgga
    11641 ccaccgatgg tgcctactta agcgcccgta acgtctccaa cctgttacgc cagaccgcga
    11701 ttaccggcat cctcgcggta ggaatggtgt tcgtcataat ttctgctgaa atcgaccttt
    11761 ccgtcggctc aatgatgggg ctattaggtg gcgtcgcggc gatttgtgac gtctggttag
    11821 gctggccttt gccacttacc atcattgtga cgttggttct gggactgctt ctcggtgcct
    11881 ggaacggatg gtgggtcgcg taccgcaaag tcccttcatt tattgtcacc ctcgcgggca
    11941 tgttggcatt tcgcggcata ctcattggca tcaccaacgg cacgactgtt tcccccacca
    12001 gcgccgcgat gtcacaaatt gggcaaagct atctacctgc cagcactggc ttcatcattg
    12061 gcgcgcttgg cttaatggct tttgttggtt ggcaatggcg cggaagaatg cgccgtcagg
    12121 ctttgggttt gcagtctccg gcctctaccg cagtagtcgg tcgccaggct ttaaccgcta
    12181 tcatcgtatt aggcgcaatc tggctgttga atgattaccg tggcgttccc actcctgttc
    12241 tgctgctgac gttgctgtta ctcggcggaa tgtttatggc aacgcggacg gcatttggac
    12301 gacgcattta tgccatcggc ggcaatctgg aagcagcacg tctctccggg attaacgttg
    12361 aacgcaccaa acttgccgtg ttcgcgatta acggattaat ggtagccatc gccggattaa
    12421 tccttagttc tcgacttggc gctggttcac cttctgcggg aaatatcgcc gaactggacg
    12481 caattgcagc atgcgtgatt ggcggcacca gcctggctgg cggagtggga agcgttgccg
    12541 gagcagtaat gggggcattt atcatggctt cactggataa cggcatgagt atgatggatg
    12601 taccgacctt ctggcagtat atcgttaaag gtgcgattct gttgctggca gtatggatgg
    12661 actccgcaac caaacgccgt tcttgatttt gataaaaatt ttctcaaagc cggttacgta
    12721 ttaccggttt tgagtttttg catgattcag caggaaaaga accatgttta ctaaacgtca
    12781 ccgcatcaca ttactgttca atgccaataa agcctatgac cggcaggtag tagaaggcgt
    12841 aggggaatat ttacaggcat cacaatcaga atgggatatt ttcattgaag aagatttccg
    12901 cgcccgcatt gataaaatca aggactggtt aggagatggc gtcattgccg acttcgacga
    12961 caaacagatc gagcaagcgc tggctgatgt cgacgtcccc attgttgggg ttggcggctc
    13021 gtatcacctt gcagaaagtt acccacccgt tcattacatt gccaccgata actatgcgct
    13081 ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg ctttttatgg
    13141 tcttccggaa tcaagcggca aacgttgggc cactgaacgc gaatatgcat ttcgtcagct
    13201 tgtcgccgaa gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg cgccagagaa
    13261 ctggcaacat gcgcaaaatc ggctggcaga ctggctacaa acgctgccac cgcaaaccgg
    13321 gattattgcc gttactgacg cccgggcgcg gcatattctg caagtatgtg aacatctaca
    13381 tattcccgta ccggaaaaat tatgcgtgat tggcatcgat aacgaagaac tgacccgcta
    13441 tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg cggcaaatgg gctatcaggc
    13501 ggcaaaactg ttgcatcgat tattagataa agaagaaatg ccgctacagc gaattttggt
    13561 cccaccagtt cgcgtcattg aacggcgctc aacagattat cgctcgctga ccgatcccgc
    13621 cgttattcag gccatgcatt acattcgtaa tcacgcctgt aaagggatta aagtggatca
    13681 ggtactcgat gcggtcggga tctcgcgctc caatcttgag aagcgtttta aagaagaggt
    13741 gggtgaaacc atccatgcca tgattcatgc cgagaagctg gagaaagcgc gcagtctgct
    13801 gatttcaacc accttgtcga tcaatgagat atcgcaaatg tgcggttatc catcgctgca
    13861 atatttctac tctgttttta aaaaagcata tgacacgacg ccaaaagagt atcgcgatgt
    13921 aaatagcgag gtcatgttgt agcgatgatg agaattgtcg gcgtcacatc agggaactca
    13981 gcacagccac ctgatgcgag caaaagcctt atccgaccga caaatcagat aaggctcaag
    14041 gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta tcctggtaca ttgcgaacag
    14101 gtagttgtta taactcttcc ctttggtcga gtagcccttc agcttgtgaa tcatcgctgt
    14161 ggcagtcact tcctgatccg ctttacgcag ctgcgcacgc gatttacgga acgaagagta
    14221 agccggatgc gtattgaggt tagtgacata ggcgctcacc gattctttga cagaactaaa
    14281 ctgtgagtac cctttcactt tacccggcgc attggtacaa cgtcctttca tgcatttcat
    14341 gccgaaaaga ttgttgttgt tgcgcgccag cttcgacgtt ccccaaccgc tttctgctgc
    14401 agccatcgtc gccaccatac tggtagggat aatgtctacg cgttcaagca aggtattcca
    14461 cgggattttt cgcgtattac cagaccactt caccttgtag cgtttagcga tgtctttcag
    14521 acgcgcacgc tcagcaggtg accattggcc ctgatactgt tttgaaatca gccagttacg
    14581 ttctgcagta atggctgcat tttggctggt aatgtaaggc attacggtcc ggagaaacgc
    14641 ctttttcctt ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac tactctttgc
    14701 actattgcga gaatactctt gtttactgct tacctgttta ttactagctt tagttaagtg
    14761 ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg aaaacaccag
    14821 agtgagtaac ataagaatca tcgccccata tcgtcgtatg ggagtcaaaa tcatcaggtc
    14881 tcctggtcgg atttaatcat tccaacacct tatatttttc acaaatttga gagttgaatc
    14941 tcaaatcata tcaaaaatag ctgtcaagag caccccaagg aatagtccaa atctgaaact
    15001 atgtcacgag ttaacgattc agattggcgc taaatcgcag aaaatgtggg ggttatcgca
    15061 aaattcagcc gttttttgcg cgcgatcgct cacccttgct tctcatcctg tggacttacc
    15121 gctcagggat gagttttgtc tggcttatcg ctggcaaact gtctgaaatc gcagcaataa
    15181 ggactcatcc gccatgaaac tcgccgcctg ttttctgaca ctccttcctg gcttcgccgt
    15241 tgccaccagc tggacttctc cggggtttcc cgcctttagc gaacagggga caggaacatt
    15301 tgtcagccac gcgcagttgc ccaaaggtac gcgtccacta acgctaaatt ttgaccaaca
    15361 gtgctggcag cctgcggatg cgataaaact caatcagatg ctttccctgc aaccttgtag
    15421 caacacgccg cctcaatggc gattgttcag ggacggcgaa tatacgctgc aaatagacac
    15481 ccgctccggt acgccaacat tgatgatttc cattcagaac gccgccgaac cggtagcaag
    15541 cctggtccgt gaatgcccga aatgggatgg attaccgctc acagtggatg tcagcgccac
    15601 tttcccggaa ggagccgccg tacgggatta ttacagccag caaattgcga tagtgaagaa
    15661 cggtcaaata acgttacaac ccgctgctac cagcaacggt ttactcctgc tggaacgggc
    15721 agaaactgac acatccgccc ctttcgactg gcataacgcc acggtttact ttgtgctgac
    15781 agatcgtttc gaaaacggcg atcccagtaa tgaccagagt tacggacgtc ataaagacgg
    15841 tatgacggaa attggcactt ttcacggcgg cgatttacgc ggcctgacca acaaactgga
    15901 ttacctccag cagttgggcg ttaatgcttt atggataagc gccccatttg agcaaattca
    15961 cggctgggtc ggcggcggta caaaaggcga tttcccgcat tatgcctacc acggttatta
    16021 cacacaggac tggacgaatc ttgatgccaa tatgggcaac gaagccgatc tacggacgct
    16081 ggttgatagc gcacatcagc gcggtattcg tattctcttt gatgtcgtga tgaaccacac
    16141 cggctatgcc acgctggcgg atatgcagga gtatcagttt ggcgcgttat atctttctgg
    16201 tgacgaagtg aaaaaaacgc tgggtgaacg ctggagcgac tggaaacctg ccgccgggca
    16261 aacctggcat agctttaacg attacattaa tttcagcgac aaaacaggct gggataaatg
    16321 gtggggaaaa aactggatca gaacggatat cggcgattac gacaatcctg gatttgacga
    16381 tctcactatg tcgctggcct ttttgccgga tatcaaaacc gaatcaacta ccgcttctgg
    16441 tctgccggtg ttctataaaa acaaaacgga tactcacgct aaagtcatcg aaggctttac
    16501 acctcgcgat tacttaaccc actggttaag tcagtgggtc cgcgactatg ggattgatgg
    16561 ttttcgggtc gataccgcca aacatgttga gttgcccgcc tggcagcaac tgaaaaccga
    16621 agccagcgcc gcgcttcgcg aatggaaaaa agctaacccc gacaaagcat tagatgacaa
    16681 acctttctgg atgaccggtg aagcctgggg ccacggcgtg atgcaaagtg actactatcg
    16741 ccacggcttc gatgcgatga tcaatttcga ttatcaggag caggcggcga aagcagtcga
    16801 ttgtctggcg cagatggata cgacctggca gcaaatggcg gagaaattgc agggtttcaa
    16861 cgtgttgagc tacctctcgt cgcatgatac ccgtctgttc cgtgaagggg gcgacaaagc
    16921 agcagagtta ttactattag cgccaggcgc ggtacaaatc ttttatggcg atgaatcctc
    16981 gcgtccgttc ggtcctacag gttctgatcc gctgcaaggt acacgttcgg atatgaactg
    17041 gcaggatgtt agcggtaaat ctgccgccaa cgtcgcgcac tggcagaaaa tcagccagtt
    17101 ccgcgcccgc catcccgcaa ttggcgcggg caaacaaacg acactttcgc tgaagcaggg
    17161 ctacggcttt gttcgtgagc atggagacga taaagtgctg gtcatctggg ctgggcaaca
    17221 gtgacttttc cggcttcccg ttcgtcagta cctcgggaag ccgccaacca ggataaaatg
    17281 tcagccctaa tcagcgttgc aggataaagc accgctcact ctccaacaga ccgatttgca
    17341 ccccagcaaa tgtagcgtta ttgttacctt ccttgctaca gagttcgaca gatatcccgc
    17401 tatgacattc tccctttttg gtgacaaatt tacccgccac tccggcatta cgctgctgat
    17461 ggaagatctg aacgacggtt tacgcacgcc tggcgcgatt atgctcggcg gcggtaatcc
    17521 ggcgcagatc ccggaaatgc aggactactt ccagacgcta ctgaccgaca tgctggaaag
    17581 tggcaaagcg actgatgcac tgtgcaatta cgacggtcca caggggaaaa cggagctact
    17641 cacactgctt gccggaatgc tgcgcgagaa gttgggttgg gatatcgaag cacagaatat
    17701 tgcactaaca aacggcagcc agagcgcgtt tttctactta tttaatctgt ttgccggacg
    17761 ccgtgccgat ggtcgggtca aaaaagtgct gttcccgctg gcaccggaat acattggcta
    17821 cgctgacgcc ggactggaag aagatctgtt tgtctctgcg cgtccgaata ttgaactgct
    17881 gccggaaggc cagtttaaat accacgtcga ttttgagcat ctgcatattg gcgaagaaac
    17941 cgggatgatt tgcgtctccc ggccgacgaa tccaacaggc aatgtgatta ctgacgaaga
    18001 gttgctgaag cttgacgcgc tggcgaatca acacggcatt ccgctggtga ttgataacgc
    18061 ttatggcgtc ccgttcccgg gtatcatctt cagtgaagcg cgcccgctat ggaatccgaa
    18121 tatcgtgctg tgcatgagtc tttccaagct ggggctgcct ggctcccgct gcggcattat
    18181 catcgccaat gaaaaaatca tcactgccat caccaatatg aacggcatta tcagcctggc
    18241 acctggcggt attggtccgg cgatgatgtg tgaaatgatt aagcgtaacg atctgctgcg
    18301 cctgtctgaa acagtcatca aaccgtttta ctaccagcgt gttcaggaaa ctatcgccat
    18361 cattcgccgc tatttaccgg aagatcgctg cctaattcat aaaccagaag gagccatttt
    18421 cctctggcta tggtttaagg atttgcccat tacgaccgag caactctacc agcgcctgaa
    18481 agcacgcggc gtgctgatgg tgccggggca caacttcttc ccgggactgg ataaaccgtg
    18541 gccgcatacg catcaatgta tgcgcatgaa ctacgtacca gagccggaga aaattgaggc
    18601 gggggtgaag attctggcgg aagagataga aagagcctgg gctgaaagtc actaaaaatt
    18661 gcctgatgcg ctacgcttat caggcctaca taatttctgc aatatattga atttggaaga
    18721 atttgtaggc cggataaggc gtttacgccg catctggcat aaacaaagcg cactattccg
    18781 gcgttgagaa acgccggaaa acgtttcaaa caggctgctg ccgtagccga tgccgctgca
    18841 ctttctcgac atccatgcac tgcaacgcct ttgtcgggca ggcttcaaca caggccggcc
    18901 ccgtctcccg atgccagcac agatcgcact taatcgccct cgcctttttg cgcgaagaaa
    18961 cgacctccat cgcaccaaac gggcaagcca gcatacagct tttacagcca atgcaacgtg
    19021 tttgttcaac gaaaatatgc ccatgttcgc ggcttatcgc gttaacaggg cagacattcg
    19081 cgcacggtgc atcttcacac tgatgacagg ctactgccgt ggtccagctg tggtctttaa
    19141 tgacacgaat acgggaaata aactcgtctg gtgacaacgc agcgcaatcc tgattctcct
    19201 gatgcgacac tgcgcaagcc acttcacagg tacggcaacc gatacatttc gtcgcatccg
    19261 caataataaa ccggttcatg atcactcctt tgcactaaaa cagcagagtg ccagagagcg
    19321 cgtccctact gctttgatcc aacgaaggaa atgttaaagt tttggcactt gtccaaagcg
    19381 ttatgtgatc gcgccctggt cgtccctgac agtaaatccc agttcattag aaatagcctg
    19441 cgcggtttca cgcagtggtt tcaggagatt tttctccccc acctgtttca gacgtgatgt
    19501 cgaaagcgaa atcgacacgg cgtacggcac ccgcccatga atattaaaca ccggaacagc
    19561 aatacaggag acgccgagtt cgttttcttc tctgtccatc gccgctccgc tttcacgaat
    19621 gtgcgccagt tcgtcgaaca tcgcgggcag ctcggtaatg gtattgcggg ttaacggctg
    19681 gatctcatgc tgatggtttt cccagtatga cttcacgtag tccgggtgac caaacgccat
    19741 gtagatcttg cccattgcgg aacagtagag cggcatatgc tggccaatat aggcacgggt
    19801 tcgcagcatc ccggttgtgg gttccagctt ataaatcaaa atagcgtgat cgtcttcgcg
    19861 gctggagaag ttaatggttt caccagtggc gatgttcagt gcctcaagat gcggagcggc
    19921 gatatgaatg atattcagcg aagacagcgc cttctgcccg acggcaataa atttggtagt
    19981 caggcggtaa ctccctgcgg cgggcgcggt ggtcacatag ccgcaggact gtaatccctg
    20041 caataagcga tggacggtac tcttatttaa accagccagc tccgaaagat gcgccaacgg
    20101 acaaccgttt ggatagttgc tcaaaatctc aatcagcatc aacccgcgaa acagactctg
    20161 gcttccggct ggacgctctt tttcctgcgc catctcgttc tctttttttc ccatcacttc
    20221 tttccccatt ttgtcgcgtc ctgatggtag cgcaaagtgt gccgtagttc acgatctcga
    20281 cagataattt ataaccactt gatttttatg tcttttgaaa ttcatcaatc agattgcttt
    20341 gttaaaaagc gatcgatata tttgaaatca agtttcgcat attgaaattt taagccaaaa
    20401 aagcgatcaa aaaaacaagg aagcctggga tgaaagtgac atttgagcag ttaaaagcag
    20461 cctttaatcg ggtcttaatt tcacgcggcg ttgacagcga aacggctgac gcctgtgcag
    20521 agatgttcgc ccgcaccacc gaatccggcg tttattctca cggcgttaat cgtttccctc
    20581 gtttcattca acaactggaa aacggcgata tcattcctga tgcccaaccc aaacgtataa
    20641 ccagcctcgg cgcaattgaa cagtgggatg cccagcgttc gatcggcaac ctgacggcga
    20701 aaaagatgat ggatcgcgcc attgaactgg ctgccgatca cggtattggt ctggtggcac
    20761 ttcgtaatgc caaccactgg atgcgcggcg gcagctacgg ctggcaggcg gcggaaaaag
    20821 gctatattgg catttgctgg accaactcca tcgccgtaat gccgccgtgg ggcgcaaaag
    20881 agtgtcgcat tggcactaac ccgctgatcg tcgccattcc ttccacgccg atcaccatgg
    20941 tcgatatgtc gatgtcgatg ttctcttacg gcatgttaga agttaaccgc ctggcgggcc
    21001 gtcagctccc ggtcgatggt ggctttgatg atgagggcaa tttgaccaaa gaacctggcg
    21061 ttatcgagaa gaatcgccgc attttgccga tgggctactg gaaaggttct ggcatgtcga
    21121 ttgtgctgga tatgatcgct actctccttt ccgacggcgc atcggttgcc gaagtcaccc
    21181 aggacaacag cgacgaatac ggcatttcac aaatttttat tgccattgaa gtggacaagc
    21241 ttatcgacgg tcccacccgc gatgccaagc tgcaacgtat catggattac gttactaccg
    21301 ctgaacgcgc tgacgaaaac caggccatcc gcttacccgg ccacgaattt actaccctgc
    21361 tggccgaaaa ccgccgtaac ggtattaccg ttgatgacag cgtgtgggcc aaaatccagg
    21421 cgttataagg agataagtca tgatttttgg acatatcgcg cagcccaatc cgtgccgttt
    21481 gcccgccgcc attgaaaagg cgctcgattt tctgcgcgct accgatttca acgccctgga
    21541 gccgggcgtt gtcgaaatcg acggcaaaaa tatttatgcg caaattatcg atttaaccac
    21601 acgcgaagcg gtggaaaacc gtccggaagt gcatcgtcgg tatatcgata ttcagtttct
    21661 ggcgtggggt gaagagaaaa tcggcattgc tattgatacg ggaaataata aagtcagcga
    21721 atcactatta gagcagcgcg atattatttt ttatcatgac agtgaacatg aatcatttat
    21781 agaaatgata ccgggcagct acgcaatttt ctttccgcag gatgttcacc gaccaggttg
    21841 tattttgcaa acagcctctg agatacgaaa aattgtggtt aaagtcgcat taacggcgct
    21901 gaattaatcg aataatcgtc tacgccagaa cgactgggta atgttattgc tctaactata
    21961 ttaataattt aggttgctgc acatgcaact tgaattatga cagacatagc ctcaaggaat
    22021 agctatgaaa aaaatacttg aagcaatact ggcgattaat ctcgccgtac tttcctgtat
    22081 tgtgtttata aacattattt taagatatgg ttttcagaca agcattttat ctgtagatga
    22141 attgtcacgt tatttatttg tctggttaac atttattggc gcgattgtgg cttttatgga
    22201 taacgcccac gttcaggtca cctttctggt ggagaaactc tcccccgcat ggcaacgacg
    22261 agtcgctctg gttacccatt ccttaatctt atttatttgt ggcgcactgg cctggggcgc
    22321 aacgctaaaa acaattcagg actggagcga ttattcaccg atcctcggtt tacccatcgg
    22381 cctgatgtat gccgcttgtc tgcccaccag cctcgttatc gcattctttg aactgcgtca
    22441 tttatatcaa ctcatcacgc gtagcaattc tctaacgtcg ccaccgcaag gagcttaatc
    22501 atggctgtgc tgatttttct gggctgtctg ttgggtggta ttgctatcgg tttgcctatt
    22561 gcctgggcac tgttgttgtg cggggcggcg ttaatgttct ggctggacat gtttgatgtc
    22621 cagatcatgg cacaaacgct ggtgaacgga gccgatagct tctccctgct ggcgattccg
    22681 ttctttgtgc tggcgggtga aatcatgaat gcgggcggct tgtcaaaacg cattgttgac
    22741 ctgccgatga aactggtggg gcataaacca ggcgggctgg gctacgtcgg cgtgctggca
    22801 gctaagatta tggcgagcct ttctggttct gccgttgcgg ataccgccgc cgttgccgct
    22861 ctactggtgc cgatgatgcg cagcgccaac tacccggtca accgggcggc ggggctgatt
    22921 gcctctggcg gcattatcgc gccaattatt cccccttcca ttccgtttat tatcttcggc
    22981 gtttccagcg gattatccat cagtaagctg tttatggcgg gcattgcccc tggcatgatg
    23041 atgggcgcaa cgctgatgct tacctggtgg tggcaggcca gtcgccttaa tttaccccgc
    23101 cagcaaaaag caacgatgca ggaaatctgg cactcttttg tctccggtat ctgggcgctg
    23161 tttcttccag tcatcattat tggcgggttt cgctccggtc tgtttacccc taccgaagcc
    23221 ggggccgttg ctgctttcta tgcgctgttt gtcgccacag ttatttaccg tgaaatgacc
    23281 ttcgccacac tttggcatgt actcattggc gcggcgaaaa ccacctcagt ggttatgttt
    23341 ctggtggcct cggcacaagt ttccgcctgg ctgattacca ttgctgaact gccgatgatg
    23401 gtttctgatt tactgcaacc gttggtcgat tcaccgcgtc tgctgtttat cgtcattatg
    23461 gtggctatcc tgattgtcgg catggtcatg gatttaacgc cgacggtatt aattcttacc
    23521 ccggtattaa tgcctttagt taaagaggca ggaatcgatc cgatttattt cggtgtcatg
    23581 tttatcatta actgctcaat cggcttaatt acaccgccta tcggtaatgt acttaacgtt
    23641 atttcagggg tggcaaaact caaattcgat gatgcagtca gaggcgtttt cccttacgtc
    23701 ctggttttat attcattatt agtcgtattt gtttttattc ccgatctcat catcctccct
    23761 ttgaaatgga ttaattaaaa ggaaaatatt atgaaattac gctctgtaac ctacgcatta
    23821 ttcattgctg gcctggctgc attcagcaca tcttctctgg cggcgcaatc tttacgtttc
    23881 ggttatgaaa cctcacaaac cgactcgcaa catattgcgg cgaaaaaatt caatgattta
    23941 ttgcaggaga gaaccaaagg cgagctgaaa ttaaaattgt ttccggatag caccctcggt
    24001 aacgcgcagg cgatgatcag cggcgtacgt ggcggcacca tcgatatgga aatgtccggc
    24061 tcgaataact ttgccgggtt atcaccagtg atgaacttgc ttgatgtccc tttcctgttc
    24121 cgcgataccg ctcacgcgca taaaacgctc gacggcaaag tcggtgatga tctgaaagcc
    24181 tcacttgaag gtaaaggact aaaagtactg gcctactggg aaaacggctg gcgcgatgtc
    24241 accaactcgc gcgcaccggt taaaaccccc gccgacctga aagggctgaa aattcgcacc
    24301 aacaatagcc cgatgaatat cgccgcattc aaagtctttg gcgctaaccc gatcccgatg
    24361 ccgtttgccg aagtctatac cgggctggaa acccgcacta tcgacgctca ggaacacccg
    24421 atcaacgtcg tctggtcagc aaaatttttc gaagtgcaga agtacctttc tctgacgcac
    24481 cacgcctatt ccccgcttct ggtggtgatc aacaaagcga agtttgatgg cttaaccccg
    24541 gagttccagc aagcgctaat ttcatctgcg caggaagcgg gtaactacca gcgcaaactg
    24601 gttgccgaag atcagcaaaa aatcatcgac ggcatgaaag aagcgggcgt ggaagtcatc
    24661 accgatctcg accgcaaagc ctttagcgac gcactgggta ctcaggtccg cgacatgttt
    24721 gtaaaagatg ttccgcaggg tgctgatcta ctgaaagccg tggatgaggt gcaataaacc
    24781 atgacgcaat actggctggg gttagattgt ggcggtagct ggctgaaagc cgggctgtat
    24841 gaccgcgaag gccgggaggc aggcgtgcag cgcctgccgc tgtgcgcatt aagcccgcag
    24901 ccaggttggg cagagcgcga tatggcagaa ctgtggcaat gctgcatggc tgtcattcgc
    24961 gaactgctta cccattccgg cgttagcggg gaacaaattg tcggtatcgg tatctccgca
    25021 caaggaaaag gcttgttttt gctggataaa aacaacaagc cgctcggtaa cgccattttg
    25081 tcctcggatc gccgggcgat ggaaatcgtt cgtcgctggc aggaagatgg catcccggaa
    25141 aaactctacc cgttgacccg acaaaccttg tggaccgggc atccggtgtc gctgttacgc
    25201 tggctgaaag agcacgaacc ggaacgctac gcgcaaattg gctgcgtgat gatgacgcac
    25261 gactacctgc gctggtgttt aaccggcgtc aaaggctgtg aagagagcaa tatttccgag
    25321 tccaacctct acaacatgag tcttggggaa tatgacccgt gcctcaccga ctggctgggg
    25381 attgctgaaa tcaatcacgc cctgccgcct gttgtcggat ctgccgaaat ctgcggggag
    25441 atcaccgctc agacagccgt actgaccggt ctgaaagcgg gtacgcccgt tgtcggcggc
    25501 ctgtttgatg tggtttccac cgcactctgc gccgggatcg aagacgaatt tacccttaat
    25561 gcggtgatgg gcacctgggc ggtgaccagc ggcataaccc gcggtttacg tgacggtgaa
    25621 gcgcatccgt atgtctatgg tcgctacgtt aacgatggtg aatttatcgt tcacgaagcc
    25681 agccccacct cttccggcaa cctcgaatgg tttaccgcac agtggggaga aatctcgttt
    25741 gatgagatca accaggccgt tgccagcttg ccgaaggccg gtggcgatct ctttttcctg
    25801 ccgttcctgt acggcagcaa cgccggactc gagatgacca gcggtttcta cgggatgcag
    25861 gccattcaca cccgcgcaca cctgttgcag gccatttatg aaggggtggt gttcagccat
    25921 atgacccatc tcaaccggat gcgcgaacgt tttactgatg tgcacaccct gcgcgtcact
    25981 ggcggcccgg cgcattccga tgtctggatg caaatgctgg cggacgtcag cggtctgcgt
    26041 attgagctgc cgcaggtgga agaaaccggc tgctttggtg cagcacttgc cgcccgcgtc
    26101 ggcaccgggg tttatcgcga tttcagcgaa gcccagcgtg atttacagca cccggtgcgc
    26161 accctgctgc cggatatgac tgtccatcag ctttaccaac aaaaatacca acgctatcag
    26221 catctcattg ccgcacttca gggctttcac gcccgcatca aggagcacat attatgagcc
    26281 gaccacttct gcaactggca ctcgaccact catcacttga agccgcacag cgcgacgtga
    26341 cgcagttaaa agacagcgtc gatattgtcg aagcgggcac cattctctgt ttaaacgaag
    26401 ggcttggcgc ggtgaaagcc ttgcgcgaac agtgcccgga caaaatcatc gttgctgact
    26461 ggaaggtcgc cgacgctggt gaaacgctcg cgcaacaggc gtttggcgca ggcgctaact
    26521 ggatgaccat catctgcgcc gcgccgctcg cgacggtaga aaaaggccac gcaatggcac
    26581 aacgctgcgg gggtgaaatt cagatagagc tgttcggtaa ctggacgctg gacgacgccc
    26641 gcgactggca tcgtattggc gtgcggcagg ccatttatca tcgcggtcgt gatgcacaag
    26701 ccagcgggca acagtggggc gaagccgatc tggcacgcat gaaggcgctt tcagatatcg
    26761 gccttgagct ttccattact ggcgggatta cccctgctga cctgccgctg tttaaagata
    26821 tccgcgtgaa agcgtttatt gccgggcggg cactggcagg cgcggcaaat ccggcgcaag
    26881 tggctggcga tttccatgcg caaatcgacg ctatctgggg aggtgcgcgt gcgtaatcat
    26941 ccgttaggga tttatgaaaa agcgctggcg aaagatctct cctggccgga gcggctggtg
    27001 ctggcaaaaa gctgcggttt tgattttgtc gaaatgtcgg tggatgaaac cgacgaacgg
    27061 ctctcacgtc ttgactggag cgccgcacaa aggacttcgc tggttgccgc gatgatcgaa
    27121 acaggtgttg ggatcccgtc gatgtgcctg tccgcacatc gtcgctttcc ctttggtagc
    27181 cgtgacgagg cagtgcgcga acgggcgcgg gaaatcatga gtaaagcgat tcgcctggcg
    27241 cgcgatctcg gcattcgcac catccagctg gcaggttatg acgtctatta cgaagaccac
    27301 gacgaaggca cccggcaacg ttttgctgaa gggctggcgt gggcagtcga acaggcggcg
    27361 gcatcacaag taatgctggc ggtggagatt atggataccg cgtttatgaa ctccatcagc
    27421 aaatggaaaa aatgggacga gatgctcgcc tcgccgtggt tcaccgtcta cccggacgtc
    27481 ggcaacctca gcgcctgggg caatgatgtt cccgccgaac tgaaactggg cattgaccgt
    27541 atcgccgcga tccacctgaa agacacccag ccagtcaccg ggcaaagccc cggacagttc
    27601 cgtgatgtgc cgtttggcga aggctgcgtc gatttcgttg gcatcttcaa aacgctgcat
    27661 gaactgaact atcgcggttc tttcctgatt gagatgtgga ccgaaaaagc caaagagccg
    27721 gtgctggaga tcattcaggc gcggcgctgg attgaagcgc gtatgcagga ggctggattt
    27781 atatgttaga gcaactgaaa gccgacgtgc tggcggcgaa tctggcgctt cccgctcacc
    27841 atctggtgac gttcacctgg ggcaatgtca gcgcggtaga cgacacgcgg caatggatgg
    27901 taatcaaacc ttccggcgtc gagtacgacg tgatgaccgc cgacgatatg gtggtggttg
    27961 agatagccag cggcaaggtg gtggaaggta gcaaaaagcc ctcgtccgat acgccaacgc
    28021 atctggcact ttaccgtcgc tatgccgaaa ttggtggcat tgtgcatacc cactcgcgcc
    28081 acgccaccat ctggtcacag gccgggctgg atctccccgc ctggggcacc acccacgccg
    28141 attattttta cggtgctatc ccctgcacgc gacagatgac cgcagaggag attaacggcg
    28201 aatatgaata tcagaccggc gaagtgatca ttgaaacctt cgaagaacgt ggcaggagtc
    28261 cggcacaaat cccggcggtg ctggtgcatt ctcacggccc gttcgcatgg ggtaaaaacg
    28321 ccgccgatgc cgtgcataac gccgtagtac tcgaagaatg cgcctatatg ggtctattct
    28381 cgcgccagct tgcgccgcag ctccctgcga tgcaaaacga actgctggat aagcactacc
    28441 tgcgtaagca tggggccaat gcctattacg ggcagtaatc cctcacgccg gggcttcatc
    28501 gccccggcac tacgaattga tatgttcctt gctgtaacgc cgcttccacg ctgctggcgt
    28561 taaaccagta tgtttctgaa aaatctgccg gaaatagcca acgtcattaa accgtattgc
    28621 atggagcagg ccgacaacgt cgagtcactg cataacctgt tgctgcccgc cgatgcgcca
    28681 tttacgacat ttgaaggcaa gggattgttc agccataaga tcctgattca gacgccaggt
    28741 tcccggtatg aacagagcaa tccagagcaa caacctttgt ggcattacga cggagcacca
    28801 gccgcaccga catccaccgg tgaattaaaa acgccagctc accccggtga caaaggtaaa
    28861 actctcatta cgatcaatca tcgggctgtc ggtaatctct tccggcagct cgctgtatcc
    28921 tgcgctcgcc atcaatacga cgtgctctcc tatcgggtat tttgccgtca ggctgacata
    28981 gggcacccag gcatcctgcg cggaataact tgccagaccg ctacgacggg actcactctc
    29041 tgaaatgcca tagtaatagt cactgaaatt ctcgtcataa tagagaacgc ccagcgcagg
    29101 tgtcagcgac aggcgaccta tctgcatttt gtggaatacc gatagctccc ccacccagcc
    29161 gttgctgtta tccagaacgt ccgcagccgc agaggctttc acggttcccc aacgctcgtg
    29221 gtgataccag gcaacccccg ccatagccgt actatcacgc ttatcaagct gttgcatggc
    29281 ataatcgtca ttatcggcag ggtcaaattc cagcggcatc caggatgcgg tcaggctaag
    29341 ttcatttttt tcactttgcg acagaataaa acctaacgtg gtctgacgaa cataaaaaga
    29401 atcaccttca taactaatca gcggaattgc ctttgtattt ttattataac cgcgataagg
    29461 cgattcatta taagccgcac cagcaccaat ggataattca gaagcagttg cgcttgccat
    29521 aaaaggcaac gcaaataacg ccacaatatt gcggttaatt aacatgtccg gtattccatt
    29581 ttaaaataag ataaaaaaga gtcggcatag taatgcttac aatttcttcg gtgcaagatt
    29641 catatttata tagtaatata taaaattata tataattggg ctgttgcgat gacaaaatta
    29701 caacttaagt accgggagtt aaaaattatc tcggtaatcg ctgccagtga aaatatcagc
    29761 catgccgcga ctgtacttgg catcgcacag gccaacgtca gcaaatacct tgctgatttt
    29821 gaatcaaaag tgggtttaaa agtctttgac cgaaccaccc ggcagctgat gctcacacct
    29881 tttggtaccg cgctgttacc gtacatcaac gacatgctgg acagaaacga gcagcttaat
    29941 aattttattg cagattataa gcatgagaaa cgtggtcggg tcaccatcta cgccccaacc
    30001 ggcataatta cctatttatc caaacatgta atcgataaaa ttaaagatat cggtgatatc
    30061 accttatcgc taaaaacctg caatcttgag cgtaatgcct tttatgaagg cgtcgaattt
    30121 cctgatgatt gtgatgtttt aatcagctac gctccaccaa aagatgaatc gctggttgcc
    30181 agttttatta cccaatatgc cgtaaccgct tatgccagcc agcgctatct tgagaaacat
    30241 cctattagcc gtcctgacga actcgaacat cactcctgta ttttgatcga ctcgatgatg
    30301 attgatgacg cgaatatctg gcgcttcaac gtcgccggga gtaaagaggt gcgggattat
    30361 cgcgtgaagg gaaattacgt ttgcgataac acgcaatcgg cgctggagct ggcacgaaat
    30421 cacctgggga ttgtgtttgc gccagacaaa agtgtgcaga gcgatctgca agacggcacg
    30481 ctggtgccct gctttcagca cccgtatgaa tggtggctgg atctggtggc tatcttccgt
    30541 aaacgggaat accagccctg gcgggtccag tatgtgctgg atgaaatgct gcgtgaaatt
    30601 cgccaccagc ttgctcagtc gcagcaactg cggcctgaac aagcggcgga aagcgaagat
    30661 taatgatcga gataaaggta atgcacccaa ctggtcatgc gtaacagcac tttgcgcatc
    30721 accgagacat ggctgaaatg atcagagtag accgccacct gggcgtaaat accgtctggt
    30781 aaagcgctca aatcagtgtg ctcatccagt tcaatggtcg cgataacgcc atctgaaccc
    30841 ggcgctgtgt ttaacgtctg taaggtgccg gtcgactgat aagctccgcc gggaacagct
    30901 ggactaatgg ctgccagctt accgctgaac actttgcctg gcagagcgtt aaataccact
    30961 tccgcatcat cgccgggagc caggcgcagc aaggagttct gacggaactg cgccacgatt
    31021 tgtcgtttct gatcgggtat aaacaccatc accggacgta gcggcagcga cgcggcatag
    31081 gtacccggac gaatcagcac ctgggtcaca taaccatcgc tcggcgcacg cactatcgtc
    31141 tgctcaaggt tatattttgc ttccgcgagc tgcgctttca ggctggcgat ttgagaatgt
    31201 tcacccaaca ccaggctatc cagctggctc tggatctgtt tttgttccgc cgccgatgac
    31261 tttacggagg cttcctgcgc cagataattt tgccgcgcca catcaatatc gcgttctgaa
    31321 aacgggttta ctttcgcctg gctgccgcgt gcgtaacgct gatactcttt agcgaattta
    31381 tcccgcgtgg cctttgcctg ctgagtattc gccgccatct catctaactc tgcgcccaac
    31441 gcccgctgtt tatgttctgc ggtaacgata tccgccatca gccgatccac ccgcgcctga
    31501 taacgcgtcg ggtccagtcg aaatagcacc tcaccttttt taatcagcgt atttttctta
    31561 tccgtcactt cgatcaccac gcctgtcacc tgtgggacaa caggaataga aataaccgct
    31621 ttttgcgctt tgaaggtata cggatggtta tagttcatta acagaattaa accactgaca
    31681 ataaatatgc cacccagggc cgctgtggga atggtccatt tatttacagg aattttgaag
    31741 atcttaaaca ttgcccatgc aaaagccaca taggtcaaaa taatcagtaa atccataagt
    31801 attactcggc agaagaggat ttgatgtcgg cgagctgttt ctccagcccg gcaatacgct
    31861 gttgtagttg cacaacggag gagtcatggt tttgcattcc ccatccccgc tccgggcggt
    31921 aaagcgtggc ccaaatccac aaaaatggcc agataacatg cagcgtaaaa agactcaccc
    31981 aaccagcaac atgaatggcg tcggcatgag gatgattacg ttttttagca atcaggtagg
    32041 gaatatcatg taaaataata atcccataga atatcaccag aaatacaaaa ataagcactc
    32101 ccagtgcaaa ataatccaga aacatatttc cctca
//
LOCUS       DAFNBJ010000042        31966 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000042 DAFNBJ010000000
VERSION     DAFNBJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.042"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            33..476
                     /gene="cmtB"
                     /locus_tag="L2X49_001819"
     CDS             33..476
                     /gene="cmtB"
                     /locus_tag="L2X49_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HBN4796912.1"
                     /translation="MRLSDYFPESSISVIYSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            504..1902
                     /gene="cmtA"
                     /locus_tag="L2X49_001820"
                     /pseudo
     CDS             504..1902
                     /gene="cmtA"
                     /locus_tag="L2X49_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
     gene            1917..3194
                     /locus_tag="L2X49_001821"
     CDS             1917..3194
                     /locus_tag="L2X49_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBN4796913.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3191..4156
                     /gene="yggF"
                     /locus_tag="L2X49_001822"
     CDS             3191..4156
                     /gene="yggF"
                     /locus_tag="L2X49_001822"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBN4796914.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4178..4687
                     /gene="fumE"
                     /locus_tag="L2X49_001823"
     CDS             4178..4687
                     /gene="fumE"
                     /locus_tag="L2X49_001823"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBN4796915.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4684..5397
                     /locus_tag="L2X49_001824"
     CDS             4684..5397
                     /locus_tag="L2X49_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBN4796916.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5369..6046)
                     /locus_tag="L2X49_001825"
     CDS             complement(5369..6046)
                     /locus_tag="L2X49_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBN4796917.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6040..6717)
                     /locus_tag="L2X49_001826"
     CDS             complement(6040..6717)
                     /locus_tag="L2X49_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBN4796918.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6705..7412)
                     /locus_tag="L2X49_001827"
     CDS             complement(6705..7412)
                     /locus_tag="L2X49_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBN4796919.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7413..7991)
                     /locus_tag="L2X49_001828"
     CDS             complement(7413..7991)
                     /locus_tag="L2X49_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBN4796920.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8014..8445)
                     /locus_tag="L2X49_001829"
     CDS             complement(8014..8445)
                     /locus_tag="L2X49_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBN4796921.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8817..9836
                     /gene="epd"
                     /locus_tag="L2X49_001830"
     CDS             8817..9836
                     /gene="epd"
                     /locus_tag="L2X49_001830"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBN4796922.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWREFGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9886..11049
                     /gene="pgk"
                     /locus_tag="L2X49_001831"
     CDS             9886..11049
                     /gene="pgk"
                     /locus_tag="L2X49_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBN4796923.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11264..12343
                     /gene="fbaA"
                     /locus_tag="L2X49_001832"
     CDS             11264..12343
                     /gene="fbaA"
                     /locus_tag="L2X49_001832"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBN4796924.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12701..13561
                     /gene="mscS"
                     /locus_tag="L2X49_001833"
     CDS             12701..13561
                     /gene="mscS"
                     /locus_tag="L2X49_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBN4796925.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13700..14335
                     /gene="argO"
                     /locus_tag="L2X49_001834"
     CDS             13700..14335
                     /gene="argO"
                     /locus_tag="L2X49_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBN4796926.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14428..15168
                     /locus_tag="L2X49_001835"
     CDS             14428..15168
                     /locus_tag="L2X49_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBN4796927.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15335..16231
                     /locus_tag="L2X49_001836"
     CDS             15335..16231
                     /locus_tag="L2X49_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4796928.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEGRQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHKPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(16228..17706)
                     /gene="scpC"
                     /locus_tag="L2X49_001837"
     CDS             complement(16228..17706)
                     /gene="scpC"
                     /locus_tag="L2X49_001837"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBN4796929.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPADSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYQDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17730..18515)
                     /gene="scpB"
                     /locus_tag="L2X49_001838"
     CDS             complement(17730..18515)
                     /gene="scpB"
                     /locus_tag="L2X49_001838"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBN4796930.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18526..19521)
                     /gene="meaB"
                     /locus_tag="L2X49_001839"
     CDS             complement(18526..19521)
                     /gene="meaB"
                     /locus_tag="L2X49_001839"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBN4796931.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DTIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIINFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19514..21658)
                     /gene="scpA"
                     /locus_tag="L2X49_001840"
     CDS             complement(19514..21658)
                     /gene="scpA"
                     /locus_tag="L2X49_001840"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBN4796932.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEEVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21862..22755)
                     /gene="argP"
                     /locus_tag="L2X49_001841"
     CDS             complement(21862..22755)
                     /gene="argP"
                     /locus_tag="L2X49_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBN4796933.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22897..23127
                     /locus_tag="L2X49_001842"
     CDS             22897..23127
                     /locus_tag="L2X49_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796934.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RLLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            23183..23842
                     /gene="rpiA"
                     /locus_tag="L2X49_001843"
     CDS             23183..23842
                     /gene="rpiA"
                     /locus_tag="L2X49_001843"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBN4796935.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24098..25330
                     /gene="serA"
                     /locus_tag="L2X49_001844"
     CDS             24098..25330
                     /gene="serA"
                     /locus_tag="L2X49_001844"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBN4796936.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25559..25618
                     /gene="ibsC"
                     /locus_tag="L2X49_001845"
     CDS             25559..25618
                     /gene="ibsC"
                     /locus_tag="L2X49_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBN4796937.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25951..26010
                     /locus_tag="L2X49_001846"
     CDS             25951..26010
                     /locus_tag="L2X49_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBN4796938.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26111..26713)
                     /locus_tag="L2X49_001847"
     CDS             complement(26111..26713)
                     /locus_tag="L2X49_001847"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBN4796939.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26734..26917)
                     /gene="ssrS"
                     /locus_tag="L2X49_001848"
     ncRNA           complement(26734..26917)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="L2X49_001848"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26959..27288)
                     /gene="zapA"
                     /locus_tag="L2X49_001849"
     CDS             complement(26959..27288)
                     /gene="zapA"
                     /locus_tag="L2X49_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBN4796940.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27456..28034
                     /gene="ygfB"
                     /locus_tag="L2X49_001850"
     CDS             27456..28034
                     /gene="ygfB"
                     /locus_tag="L2X49_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBN4796941.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28060..29385
                     /gene="pepP"
                     /locus_tag="L2X49_001851"
     CDS             28060..29385
                     /gene="pepP"
                     /locus_tag="L2X49_001851"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBN4796942.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29382..30560
                     /gene="ubiH"
                     /locus_tag="L2X49_001852"
                     /gene_synonym="visB"
     CDS             29382..30560
                     /gene="ubiH"
                     /locus_tag="L2X49_001852"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBN4796943.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAVL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30583..31785
                     /gene="ubiI"
                     /locus_tag="L2X49_001853"
                     /gene_synonym="visC"
     CDS             30583..31785
                     /gene="ubiI"
                     /locus_tag="L2X49_001853"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBN4796944.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDTTLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELVAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAAMMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31859..31942)
                     /locus_tag="L2X49_001854"
     CDS             complement(31859..31942)
                     /locus_tag="L2X49_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796945.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
BASE COUNT         7639 a         7910 c         8625 g         7792 t
ORIGIN      
        1 acggtaccct aaaaataaaa taaggataat ttatgcggct tagtgattat tttccagaat
       61 catcaatctc agttatatat tcagcaaaag attggcagga agctatcgat ttctcgatgg
      121 tatcattgct ggataaaaac tatatcagcg agaattacat tcaggcaatt aaagattcca
      181 ccattaacaa tggcccttat tatattctcg caccaggcgt ggcaatgcct catgcgcgac
      241 cggaatgtgg ggcgcttaaa accgggatgt ctttgacatt acttgaacaa ggtgtttatt
      301 ttccggggaa tgacgaacca attaaattac tcatcggact ctctgctgcc gatgccgatt
      361 cgcacattgg cgctattcag gcgttaagtg aattactgtg cgaagaagaa atactcgaac
      421 aactcttaac agcatcatca gaaaaacaat tagcggacat tatcagccgc ggataattac
      481 tctctcctct acaggataat attatggaaa acaagtctgc tcgtgcaaag gtccaggctt
      541 ttgggggctt ttgggggctt tttgactgca atggtcatcc ccaatattgg tgcttttatt
      601 gcctggggct tcataactgc gttatttatt cccaccggtt ggctgccgaa tgaacatttc
      661 gccaaaattg tcggcccgat gattacctat ttattgcccg tgatgattgg ttctactggt
      721 ggtcatctgg tcggcggtaa acgcggggcg gtcatgggcg gaataggtac tattggtgtg
      781 atcgttggcg cagagatccc gatgttcctt ggctcaatga tcatggggcc gctcggtggg
      841 ttggtcataa aatatgtcga taaggcactg gaaaaacgca tacctgccgg ttttgagatg
      901 gttatcaata acttctcatt aggtatcgcg gggatgctcc tttgtctgct gggttttgaa
      961 gtgatcggcc cggcggtgtt aattgccaat actttcgtca aagagtgtat cgaggcgctg
     1021 gtacatgcgg gttatctgcc tctgctgtca gtcatcaatg aaccggcgaa agtgcttttc
     1081 cttaataatg cgatcgatca gggcgtctat tatccgctgg gaatgcaaca ggcttcggtt
     1141 aacggtaaat ccatcttctt tatggtggcc tctaacccag gtccgggcct ggggctgctg
     1201 ctggcgttta ccttgtttgg taaagggatg agtaaacgtt ctgcgcccgg ggcgatgatt
     1261 attcacttcc tcggtgggat ccacgaactg tatttcccat atgtgctgat gaagccgctg
     1321 actattattg ccatgattgc gggcggtatg tctggtacct ggatgtttaa cttactggac
     1381 ggtggtctgg tggctggccc aagtccgggg tctatctttg cttacctggc actgacgccg
     1441 aaaggctcgt tcctggcgac aattgccggt gttacggtag gtaccctggt gtcctttgct
     1501 attacttcgc tgatactgaa gatggaaaaa acggtggaaa cggagagcga agatgagttt
     1561 gctcagtcag ccaatgcggt taaggcgatg aaacaagagg gtgcattctc gttaagcagg
     1621 gttaagcgta tcgcctttgt ttgcgatgcg gggatgggct ccagtgcgat gggcgcgacc
     1681 accttccgta aacgcctgga aaaagcgggg ctggcaatag aagtaaaaca ttacgcgata
     1741 gaaaacgtgc ctgcggatgc cgatatcgtc gttactcatg ccagtctgga agggcgcgtg
     1801 aaacgtgtta cggataaacc gctgatattg attaataact atattggcga tccaaaactc
     1861 gacactttat ttaatcaatt aaccgccgaa cataaacact gattacagag gtaaaaatga
     1921 aaaccaaagt tgctgctatt tatggcaagc gggatgtccg tctgcgcgaa tttgaactgc
     1981 cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc tgtttatcga
     2041 cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat ttagaaaatc
     2101 atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt aaaaatctca
     2161 ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg ttaccaagcg
     2221 gatattcagc aggctacagc tacgaatatt ttggcggtaa cgccacctat atgattattc
     2281 ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat tttgctgcgg
     2341 cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat tatcacacca
     2401 cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt gcactgctgg
     2461 cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt ggcatacaac
     2521 cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg cagaagctgc
     2581 tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat accaaaggga
     2641 tgagcgatcc ggtccagacg ctgcgggcgc tgacaggaga tgtcgggttc gatgacattt
     2701 ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg gcggaagatg
     2761 gctgtctgaa cttctttgcc gggccgacgg ataaaaactt caaagtgccg tttaatttct
     2821 acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca acggacgaca
     2881 tgaaagaggc gattgcgtta agcgccacgg ggcagttgca gccgtccttt atggtcacgc
     2941 atatcggtgg gctggatgcg gtgccagata ccgtgctcaa tctgccggat atccctggcg
     3001 gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc gattttgccg
     3061 aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag gaaacgcacg
     3121 gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt gatatcgggg
     3181 aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac aggcagcgtt
     3241 ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc tggcggtcac
     3301 cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta tcggcgaagg
     3361 agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag gcgatgggcc
     3421 agaagtcgat atcgcggttg acccgattga aggcacgcgg atggtagcga tggggcagag
     3481 caatgcgctg gcggtcatgg ctttcgcccc tcgagatagc ctgttacatg cgcccgatat
     3541 gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc tgtctttgcc
     3601 tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg acaagctgcg
     3661 catggttacg ctggataaac cgcgcctgag tgccgcgatt gaagaagcaa cacagctggg
     3721 cgtgaaggtt ttcgctctgc cggatggcga tgtcgccgcc agcgtgctga cctgctggca
     3781 ggataatcct tacgacgtga tgtacaccat cggcggtgcg ccagaaggcg tgatttctgc
     3841 ctgtgccgtt aaagcattag gtggggatat gcaggccgag ctgattgact tttgtcaggc
     3901 taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc gttgtaaggc
     3961 aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg ggaacgatat
     4021 cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc aacagacggc
     4081 gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt gtaatataat
     4141 agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga cagaagatga
     4201 tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa ctgcgcattc
     4261 tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata acgatgaaga
     4321 gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg acgatattga
     4381 tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt atgccgatat
     4441 cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc ccggatttgc
     4501 cgatgacatg acctgggatt ttatcagcaa cgtcaatagc ataacctgca atgcgacgct
     4561 ctatgacgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag aggcgcgttt
     4621 tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa aggaattaac
     4681 gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta ccatgatgaa
     4741 gagatagaac gcgtgcataa accgttgctg cgtatgctgg cggccttaca gacggtaagt
     4801 ccgcagcgac gtacggtggt ttttctttgc gctccgccgg ggacgggtaa atctacctta
     4861 actaccttct gggaatatct cgcgcaacaa gaccctgaat tacccgctat ccagacgctc
     4921 ccgatggatg gttttcacca ttacaatagc tggctggatg cgcatcaatt gcgtcccttc
     4981 aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgtg ccaggtagtg
     5041 gaaggggatt gtacgtggcc gcagtatgat cgacaaaagc atgatcctgt tgaagatgcg
     5101 ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct ggacgacgag
     5161 aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc gccagctact
     5221 gcgttgcggg aacgactggt agggcgcaag ctggcgggag gggtatcgct ggcagacgca
     5281 gaagcttttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga agagagtctg
     5341 cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt ggattaacgt
     5401 tcctgattag accatcttcc agtcgcacca cacgagagaa atgacgccgg gtaaatgcgg
     5461 cgtcatgact gattgctacg acgctcgtgc ctcgctggcc gcacttctcc agccagcttt
     5521 caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc agcaatagtg
     5581 gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca ctatgcagat
     5641 ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca gcatgggtac
     5701 gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg atctcgtcag
     5761 cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca acctttgccg
     5821 cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc gtaccggctg
     5881 tgggcgtaag aagacctgcc attactcgta acagcgtcga ttttcccgcg ccattgtcgc
     5941 cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa agacaatccg
     6001 ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg gctggagcgc
     6061 tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc gatgacgttc
     6121 aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct gcaacagcat
     6181 ttcgctggcc tgcggagtca ggcggctaaa ggcttcatca agaattaaga gctttggctg
     6241 catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga gtgttgccgg
     6301 gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa tccgcgccat
     6361 aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt cttccacgct
     6421 aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga tggtggcgga
     6481 ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg tgcctgttat
     6541 ctctcctggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg tacttttgcc
     6601 actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa agtcataaca
     6661 gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg taaccatatc
     6721 cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac gcgttgtagg
     6781 gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa tgcccgcata
     6841 tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag cggaataatt
     6901 aacgcgggca tcgcacgtaa acgctgatac cagccctcat ccagcggcac gccgcgggca
     6961 cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac caataacggc
     7021 ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc gcgaataaaa
     7081 cattgcacgg ggacgtactg catccacaac tgtgaggttg aaacaatcgc cagaatccgc
     7141 aaccagagcg taatagcatg cgaccaccgt tctggcgaac gcggcgtgcc gcttagccat
     7201 tctgttagcc agccaccgtg taccagccac agcccggctc ccagcgagaa catcagccag
     7261 acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcaaaa aaaggtcgcc
     7321 gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca ggccgccaat
     7381 gcccatagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat tgccggaaaa
     7441 tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac cgctgtaaga
     7501 attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac caggttttgc
     7561 cccatagagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac accgccgaac
     7621 agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat cacgccgctg
     7681 acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc ggcgaccagg
     7741 ccgatgacca tcgcgacggg ggcaaatgcg gcggcgatcg ggtcggtcag caatccccag
     7801 agtaaattgg tcagtaagcc ggttagcatc ccgatcaccg gaccaagcag taccgcgcta
     7861 ataagggtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc cagttgcccg
     7921 ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact ggaaaagtgg
     7981 ctacgcgcca taagaaaacc tctctgaaag tagttattta acgggggaaa tatgcaactc
     8041 gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa ggctaccgat
     8101 cactagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc caagcatcgt
     8161 gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca gcgtgccgtt
     8221 tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc cggcatagcg
     8281 caaggcgacg tcagtcaggc taccggtaca gcctgcaatc caggcggcat gaagttgctg
     8341 ctgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagca ggcgcagtgc
     8401 gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag tactccgaaa
     8461 ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata taagtcaggg
     8521 tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc aaagtggaat
     8581 aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gatatttgtt ttctttgact
     8641 ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt tcagtcgatt
     8701 aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt ccccggacga
     8761 tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg agatctatga
     8821 ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt cgtgctttgt
     8881 atgaatcagg acgccgtgcg gaaattaccg tggtggcaat caacgaactg gcggatgctg
     8941 cgggcatggc gcatttgttg aaatatgaca ccagtcatgg ccgttttgca tgggaagtac
     9001 gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg catgaacgtt
     9061 cactgcaatc gctcccctgg cgtgaatttg gcgttgatgt agtcctcgac tgcaccggcg
     9121 tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa aaagtgctct
     9181 tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc aatcaggatc
     9241 aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat tgcataattc
     9301 ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg accacaattc
     9361 actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg cgtcgcaccc
     9421 gggcagccag ccagtcgatc attccggtcg atactaaact ggccgccggt atcacacgat
     9481 tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca accataaatg
     9541 tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat gaagtcaacc
     9601 tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat acggaattgc
     9661 cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat ggcacccaaa
     9721 cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac gaatggggct
     9781 ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc aggtaagacg
     9841 caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc tgtaattaag
     9901 atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct gaacgtacca
     9961 gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc gaccatcgaa
    10021 ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg tcctaccgaa
    10081 ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa agacaaactg
    10141 tctaacccgg ttcgcctggt taaagattac ctcgacggcg ttgacgttgc tgaaggtgaa
    10201 ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga cgaaaccctg
    10261 tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcgttcgg tactgctcac
    10321 cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg cgcaggcccg
    10381 ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc tcgcccgatg
    10441 gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga ctccttgtct
    10501 aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat cgcggcacaa
    10561 ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc taaacgtctg
    10621 ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga gttctctgaa
    10681 actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca gatcctggat
    10741 atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa aaccattctg
    10801 tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac tgaaatcgtg
    10861 gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga cactctggca
    10921 gcaattgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg cggcggcgca
    10981 ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga agagcgcgct
    11041 aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc ttttagagca
    11101 cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag agtgaatccc
    11161 caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca catgcaactt
    11221 gaagtatgac gagtataagg cccgacgata caggacaaga gacatgtcta agatttttga
    11281 tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc aggtagcaaa
    11341 agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca tcaacgccgt
    11401 actggaaacc gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca acggtggtgc
    11461 ttcttttatc gctggtaaag gcgtgaaatc tgacgttccg cagggcgctg ctatcctggg
    11521 cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg ttccggttat
    11581 cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc tgctggacgc
    11641 gggtgaaaaa cacttcgctg ctaccggtaa gccgctgttc tcttctcaca tgatcgacct
    11701 gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg agcgtatgtc
    11761 caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag aagacggcgt
    11821 ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg ttgattacgc
    11881 atacaccgag ctgagcaaaa tcagcccgcg cttcaccatc gcagcgtcct tcggtaacgt
    11941 acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc gtgattctca
    12001 ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg tattccacgg
    12061 tggctccggt tctactgctc aggaaatcaa agattccgta agctacggcg tagtgaaaat
    12121 gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact actacaaagc
    12181 gaacgaagct tatctgcagg ggcagctggg taacccgaaa ggcgaagatc agccgaacaa
    12241 gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga ttgctcgtct
    12301 ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagatattc ctttctgctt
    12361 atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaaattgcc tgatgcgcta
    12421 cgcttatcag gcctacacgt atcctgcaat atattgaatt tgcaggtttt tgtaggccgg
    12481 ataaggcgtt cacgccgcat ccggcatgac aaagcgcaca ttgatatctg tctgagcccc
    12541 gctctgcggg gattttttcg ccaaaagcaa ggtgattcag atgagaaatc tgtgatctat
    12601 ttggcaaaat tatgctttat tgtttaccct tgtcagactg cccgtcataa ggcggcggag
    12661 tgtatttctc cattttgagt cagttgaaaa ggaatattga atggaagatt tgaatgttgt
    12721 cgatagcata aacggcgcgg gaagctggct ggtagctaac caggcgctgc tgctaagtta
    12781 tgcagtaaac atcgtggcgg cactcgcgat catcatcgtt ggtttgatta tcgcgcggat
    12841 gatttccaac gcggtgaatc gcctgatgat ctcccgtaaa atcgatgcca ctgttgctga
    12901 ttttctttct gcattagtcc gttacggtat tatcgccttt acgctaatcg ctgccctggg
    12961 acgcgtgggt gtacaaacag cgtcagtcat tgctgtactc ggtgccgcag gcttagctgt
    13021 tggtctggct ttgcaggggt cactttctaa cctggccgct ggcgtgttac ttgtcatgtt
    13081 ccgcccgttc cgtgccggag aatatgttga cctcggcggc gtagccggta ctgtgctgag
    13141 tgtgcagatt ttctccacca ccatgcgtac tgcagacggt aaaattatcg ttattccgaa
    13201 cggtaaaatt attgccggaa atattattaa cttctcccgc gagccagttc gccgtaacga
    13261 atttattatt ggcgtggcgt atgattccga tatcgatcag gttaagcaga tcctgaccaa
    13321 tattatccag tctgaagatc gcattttgaa agatcgcgaa atgactgtgc gcctgaacga
    13381 acttggtgca tcgtcgatta atttcgtggt ccgcgtctgg agcaacagcg gcgatctgca
    13441 aaacgtgtac tgggatgtgc tggagcgtat taaacgtgaa tttgatgccg ccggtatcag
    13501 cttcccgtac ccgcaaatgg atgtgaactt taagcgggtg aaagaagaca aagctgcgta
    13561 atcaacgctg aggccagata atactctctg gcctctctct tattagttat tctgattgcc
    13621 aattaatatt atcaatttcc gctaataaca atcccgcgat atagtctctg catcagatac
    13681 ttaattcgga atatccaacg tgttttctta ttactttcaa ggtcttgctc ttggggcagc
    13741 tatgattctg ccgctcggcc cacaaaatgc ttttgtgatg aatcagggca ttcgtcgtca
    13801 gtaccacatt atgattgcct tactttgcgc tatcagcgat ttggtcctga tttgcgcggg
    13861 gatttttggt ggtagcgcgt tattgatgca gtcgccgtgg ttgctggcgc tggtcacctg
    13921 gggcggcgta gtcttcttgc tgtggtatgg ttttggcgct tttaaaacag cgatgagtag
    13981 taatattgag ttagctagtg ccgaagtcct gaagcaaggc agatggaaaa ttatcgccac
    14041 catgttggca gtgacctggc tgaatccgca tgtttaccta gatacttttg ttgtactggg
    14101 cagccttggc gggcagcttg acgtcgaacc aaaacgttgg tttgcgctcg ggacaattag
    14161 cgcttctttc ctgtggttct ttggtctggc tattctcgca gcctggctgg caccacgcct
    14221 gcgcacggca aaatcacagc gcattatcaa tctggtagtg ggatgtgtca tgtggtttat
    14281 agccttgcag ctggcgagag acggtattgc tcatgcacaa gccttgttca gttaggcgct
    14341 atctgatgga aaaataaaac agaggcgcta agcttgcctc cagaggtcct gaattttggg
    14401 caagtcaatt aacacggaga gactaacgtg aagttcaaag ttatcgccct ggcggcatta
    14461 atgggtatta gcgggatggc agcgcaggct aacgaattgc cggatggacc gcatattgtc
    14521 acctccggta cggcaagtgt ggatgcggtg ccagacattg ccactcttgc gattgaagtt
    14581 aacgtggccg cgaaggatgc cgctacagcc aagaaacagg cagatgagcg cgtcgcacaa
    14641 tacatttcct tccttgaact caatcagatc gcgaaaaaag atatcagctc agcgaattta
    14701 cgcacccagc cagattatga ttatcaggat ggtaaaagta tcctgaaagg ctaccgcgct
    14761 gtgagaacgg tggaagtcac gctccgtcag ttagacaaac tgaattcctt gctggatggc
    14821 gcgctgaagg cgggtcttaa cgaaattcgt tcagtgtcgc tgggcgtggc gcagccggat
    14881 gcctataaag acaaagcgcg taaggcagcg attgataacg cgattcatca ggcgcaggaa
    14941 ctggcgaacg gctttcatcg taaactgggg ccggtatata gcgtgcgcta ccatgtttcc
    15001 aactatcagc ccagcccaat ggtgcggatg atgaaagccg atgccgcgcc ggtgtctgcc
    15061 caggaaactt acgagcaggc cgctattcag tttgatgatc aggtcgatgt ggtcttccag
    15121 ttagaacctg tggatcaaca acccgctaaa acacctgcag cacaataata cttgtagccc
    15181 gacgtattcg tcgggctaat gcctttaccc gatattgcct cctttttctg taattttctg
    15241 taaaaccatg acgtaatcac ctatctttgc tcgcggtttc atcaaggata ggctgactga
    15301 aacggttaag cggacaagga tgcggggagg ggggatggac atttttattt cgaaaaagat
    15361 gcgcaatttc attttattag cgcaaaccaa taatattgcc cgggcggcag aaaaaatcca
    15421 tatgacggct tcgccatttg gtaaaagcat tgccgcgctg gaagaacaaa ttggctatac
    15481 gctatttacc cgcaaagata ataatatcag cctcaataaa gccggtcagg aactgtatca
    15541 aaaactgttc ccggtttatc aacgactttc tgcgattgat aatgaaatcc ataactcagg
    15601 gcgtcgttca cgagaaattg tgattggcat agataatact tatccgacaa ttattttcga
    15661 tcagttgatt agtctcggag ataagtatga aggcgtgacc gcccagccag ttgagttcag
    15721 cgaaaacggc gttatcgata atctttttga ccgtcagtta gattttatta tctccccaca
    15781 gcatgtatct gcacgagttc aggaactgga aaacctgact atcagtgaat taccaccgct
    15841 gcgattgggc tttctcgttt ctcgccgtta tgaagggcgt caagagcaag agctgcttca
    15901 ggagctgccc tggctacaaa tgcgctttca aaaccgggct aacttcgaag caatgattga
    15961 tgcgaatatg cgaccttgtg ggatcaatcc caccattatt tatcgtccct acagttttat
    16021 ggccaaaatc agcgccgtgg agcgcggaca ttttctgacg gtgatcccgc attttgcctg
    16081 gcgactggtg aatccggcaa cgctgaaata ttttgatgct ccgcacaagc cgatgtatat
    16141 gcaggaatat ctttattcaa tcagaaatca tcgttatacc gccacgatgc ttcagcatat
    16201 tgctgaagat cgtgacggga cgagtcatta acccagcatc gagccggttg caattaaatt
    16261 acggtgtaag tcgaagacgt ggctaagatc gtggtgaata tgtccgccag gcgcattttc
    16321 cagatagcga tgcagataat cctgatacat aggatgtgca caattatcaa tgatagtgcg
    16381 ggcgcgttga agcggggaaa gaccgcgcag atcggcgatc ccttgttcgg tgatgatcac
    16441 tttgacgctg tgttcgctgt gatcaacatg gctgcacatt ggcacgacgg ttgagatctt
    16501 gccttcttta gcaatcgacg gggccataaa gatcgacaga tacgcgttgc gttcaaaatc
    16561 accgctgcca ccgatgccgt tcatcagatc gaccccggct acgtgcgttg agttggcatg
    16621 cccgtaaata tcaaactcca ggccgacgtt cagagcgatg acgcccagac gacggatgat
    16681 ttccgggtta ttggaaatct cctgcggacg caacacaatg cggctggcaa agtaatccat
    16741 attgtcgtaa atcttgcgca gggaatcggc cgagattgtc aggctggagg cgctggcccc
    16801 gctgattttg ccggtttcca gtaaatgcac caccgattcc tgtagcactt ccgaatacat
    16861 cataaacgga ggaatttccg ggttttcccc cagacgcgcc attaccgcat tattgatatt
    16921 gcccacgcca ctttgcagcg gcagaaattc cggcggaata cgcccatgcg ccatttcctg
    16981 caacaagaac gtgaccacgt tatcggcaat ctgctggcac atgggatttt gcttatccag
    17041 catattaccg gcgtcgggca agttggtttc cacgacggcg acaatctttt tcggatcgat
    17101 ttgcacatag cgggtaccga cgcgatccat tgcatggaag atcgacacgc tattgcgccg
    17161 cggtggcgcg ccaggaatca caatatccgc cagttctgca acgcgcggat cgtgatagtg
    17221 attgagttca atgatcactt tcttcgcccg cagcagccag gtcggcgcat taccgatccc
    17281 gctggttaac cagactcgac catccggtgc cagtgccgat gcttcaatga cggcaacatc
    17341 aatgtcgccg aagaaaccgt aattgaccat ttgcgccact tcgctcaaat gcaggtcaac
    17401 gaaactcacc gcgccctgat tgatcttttt acgtaaaccg gacgatgttt gatatggtgc
    17461 acgccaggaa acagcatcgg cgtcagaaag tacatcgtca gcggcggcgc tgattgacgc
    17521 acccgtcaga aggcgaattt gatacggctt tttggcctca tgctgttcgt tagctctgcg
    17581 ggcaatcgcg gtgggtaggg ctttcggcga atccgccggg gtaaagccgc taaatgccac
    17641 catgtcgtta tgctggataa tttctgccgc ttcattggcg gtcatccttg tccactgagt
    17701 ttccattttt actccttcgt tcgcagggat taatgaccaa cgaaattagg tttacgtttt
    17761 tcgaggaaag cgttcatccc ttcctggtaa tcttcgctgt catacaccgc gcggcgcatc
    17821 ccctgaatac gttcaaattc atcggagttc atggtgtgtg cttcgcccag tacacgcagc
    17881 tcttctttga taacggcaat ggctaacggc gctttctcag agatgtggtg cgccatttgt
    17941 aaggtgaaat cttccagttc ttccacttcc acaacatggt tgaggatgcc gacagccagc
    18001 gcgcgctggg cggtgattgg cgaagcggta aaaatcagct ctttgacaat gtggaagccc
    18061 gcgtcgcggg tcaggttgtg aatgccgacc aggttatacg ggacgccgag gtttacaggc
    18121 gtcattgaga aggttgaggt actggcggcg atgatcagat cggaactcat gatcatttca
    18181 aatgcgccac cccaaacact accttccacc atcgaaatga tcggtttcgg gaatttttgg
    18241 atcatgcggg tgatttgacg caatggatca tcataggaga gcggatcgcg accgccagac
    18301 ggcagttcgt gaatatcgtg gcctgcggag aagactttgg atccactcgg cgcgcgcaaa
    18361 atgatacagc gaatttccgg ccggttgaga tcgcttaatg cctgcataag atcatcaata
    18421 aagactttac ttaaggcatt aagtttgcgg ccatagttaa actcaatgac cgccactttg
    18481 ttgatagtga caacgttaac atactgataa gacataaaaa ttcctttaat caaaatattg
    18541 cgtctggata aattcactga gctgccgcag gccggtgcgc ggtgagagcg tattgttttt
    18601 gaccgctaaa agcgtctggc ggtaatagcg atcgaaatct tcattcgcga acaggtgatt
    18661 cagtacttct tcttcggtct gcttacgcag ccattccacc gattgttgtt gccgcacttg
    18721 ttgtaaacga ccactggcag ttagcgcggt tttgaagttg atgatggcgt gccagatctc
    18781 atcgattcca cgtttttcca gtgcgctaca agtcagaacc cgtggctgcc attcgtcgta
    18841 tttacgtcgc agaatatgca gggcactctc gtacatatgc cgggcaatgg cgacattggt
    18901 atggttatcg ccatcgtctt tgttgataac gagcagatca gccacttcca tcagcccttt
    18961 tttaatgccc tgcagatcat cgccgccacc ggcaatttgc aacgagataa aacagtccac
    19021 catgcgggcg acttccgttt ccgactgccc gacgccaacc gtttcgacaa tcactacgtc
    19081 ataacccgcc gcttcgcata acagcattaa ttcccgcgct cgctgactgg caccgcccag
    19141 atgaccggag gatggtaccg ggcgaataaa cgccgcttcg gcacgcgcca ggtcattcat
    19201 gcgggtttta tccccgagaa tgctaccgcc agtgaccggg ctgctgggat cgaccgcaat
    19261 aaccgcgacc tttaatccct ctcgaatcaa caacatgcca aaggcctcaa gaaaggtact
    19321 tttccccgcg ccgggggtgc cggtaacgcc cagtcgcagg gtgttaccgc agtacggcat
    19381 aatggtatca agcagctgcg tacttagtgc ctgatgacgc gggtgacggc tttccaccag
    19441 cgtcatggcc tgggcgagtg tggcacgctc accctgacgt aagcggcgaa tactttctgc
    19501 cagcgtggct tcattaatca tgatgctggc ttatcagatt cagtacgtcg cgcacactgt
    19561 cgagcatagg tgtacctgga ccataaatcg ccgccacgcc gcgctcttgc aggaaggcgt
    19621 aatcctgcgg cggaatgacg ccacccgcga ccacgcagat atcttcgcgt ccccattttt
    19681 tcagcgcttc gaccagttcc gggatcagcg ttttatgacc ggcagccagt gaggatgcgc
    19741 ccactacgtg aacgtcgttt tctacggcca ggcgggcgat ctcttcaggt gtagagaaca
    19801 tcgggcttaa atctacgtcg aaaccgagat cggaataggc gctggcgatc actttcgcgc
    19861 cgcgatcgtg tccatcctgg cccatcttag cgatcagaat gcgcgggcga cgaccattgt
    19921 cggcaaggaa ctgctccgtt tgcgcaacaa tggcatcgaa ctcggaggcc gatttctccg
    19981 actgatgata gctttgcgca attacgccgg taacacactg gcttggcacc agataacggt
    20041 cgaaagcggc ttccagcgca tcggaaattt cacccagggt ggcgcgaacg cgagcggcat
    20101 taacagcggc agccagcagg ttttcgttat gctgtgcggc gtgagtcagg gcgttcaacg
    20161 cggcggttac ggcggcatca tcacgggtgg cgcgaatgcg ttccagcgaa gcaatttgct
    20221 cgttacgcac catcacgttg tcgatctcaa gtacatcggt ttcgtcttcg tgatccagtt
    20281 tgtacttgtt gacaccaacg atgacacgct tgccctggtc gatcagcgac tgttcgcgcg
    20341 ctgaggcctc ttcgatcatt cgttttggca gacctgcttc gatcgctttc gccatgccac
    20401 cggcttcgtc gatctgttgg ataatagctc tggcttgttt gacgatttga tcggtcagcg
    20461 actcaatgta ataggatccg gccagtggat cgacggtgcg gcagagttct gattcttcct
    20521 ggatgatgat ctgggtgttg cgggcaatgc gtgctgagaa atcggtaggc aaaccaagcg
    20581 cttcgtcaaa ggcgttggta tgcagtgact gagtaccgcc cagcgtcgca gccagcgctt
    20641 caatggtggt gcggataacg ttgttatacg gatcctgttc agtcaggctc cagcctgagg
    20701 tctggcagtg ggtacgcagc gccagtgatt tcgggtcctg tgcgccaaat ccactgacct
    20761 cttcgctcca taaataacgt gccgcacgca acatggcgac gttcataaac agatccatgc
    20821 cgatgccgaa gaagaacgac aggcgaggag cgaagtcatc aattttcagt ccggcagaga
    20881 ttgctgcttt gatgtactca atcccatcag cgagcgtaaa tgctacctgc tgcacgcagt
    20941 tggcacccgc ttcacccatg tggtaaccgc tgatactgat ggtattaaat cgcggcatgt
    21001 tgccggaaca ccaggcgatg atgtcggcga taatgcgcat tgacggtttt ggcgggtaaa
    21061 tataggtgtt gcggcagagg tactctttga gaatatcgtt ttgaatggtg ccggtcagtt
    21121 tatcaggtgt aacaccttgc tcttctgcgg cgacgatata aaacgccagt actggtagca
    21181 ctgcgccatt catggtcatc gaaaccgaca ttttatccag cgggatctgg tcgaacagga
    21241 ctttcatatc ttccacggtg tcgatagcga cgcccgcttt gccgacgtca cccgccacgc
    21301 gtgggttatc ggagtcgtag ccacggtggg tggcaaggtc aaacgcaacg gaaagacctt
    21361 tttgcccggc ggccaggtta cggcgataaa aagcgttgga ctcttttgct gttgaaaaac
    21421 cagcatactg acggatggtc cacggttggg cggtatacat agtggcacgc gggccacgaa
    21481 cgtagggcgg caaaccagga agggtacctg tcacctccag attatcgaga tcggcttcgg
    21541 tatacagcgg cttgatggcg atcccttccg cggtttgctg aaccagcgag tcgacagttt
    21601 tctcccgacg gctcaattcc ttgttggcaa gctgttgcca ctcctgctcg ttagacatta
    21661 tttgctccgt aaaacgcaat caaaatgagg gtatttgcgc ttacggtgag ctttttacgt
    21721 gggtttgtcg ccagcaaaaa aaatgcattc agacgtcgtt ttttgccgcc tgagatttgt
    21781 ctattttgtg atgtgattca aggaatacaa aaagaagtga ggacaacgcc tgatatgtgc
    21841 tatcaggcat tatttgatgg attaatcctg acgaaggact ttgtgaccat aatcgagtaa
    21901 cgcatcagtg actttacgca tcatgcggct ttcaggagca aagcggtgcc agtagagcat
    21961 ccgtcgttga aatagcccag gcgttaagtc aatcagttca ccgctggcca gctctttctc
    22021 gatttgcagg tgcgggatca tacagcaggt ggtgccctgg cgagcaagtt gtacgaacgc
    22081 ttctgaagaa ttaacgatat ggcagggcac gctgcctgga ggcagatcga agttttgctg
    22141 caaaaaggcc tggtgcatat cgtcaagatg gtcaaacgcg accactggcg ctttcagtaa
    22201 tgccgaacgc gttacgccgt tagggaaata tttttcggca aagggttttg agctgacgaa
    22261 cagatagtcg agcgcaccaa gtttatcgac aagacaactc ggcagcgcct gatgttgaat
    22321 actcaccgcg ccgaccactt cgccgcggcg cagacgttcc tgagtgcggg tttcatcttc
    22381 tacctgcaag ttgaggcgga taggcgaatc agccaacaca ggagccagtg caggaagcaa
    22441 ccacgtcgcc agactgtcgg cgttgaccgc cagtgaaagc agcagcggag tcgaaccggt
    22501 ttgttcatcg cccagccact cttcttccag caactccacc tggcgcagca gtgccagcag
    22561 tttttgccct tgttccgtcg ggcgcggcgg tacggtacgc accaacagcg gctgcccgaa
    22621 catattttcc agttgcttaa tgcgctgtga gacggctgat tgtgtaatgc acagcttttg
    22681 tgccgcgcgc tcaaatcctc gttcacgtat caccgcatcc agtgcctgta atgttctgta
    22741 gtccgggcgt ttcattgctg ttgtctgctc ctggtaaatg ctttttcctg cactatgaca
    22801 caattttgtg tcaggttgca tataccgaag ccgtcggcgg gtaacatttg tattcgtggt
    22861 taaacgcgca gcttaaaaga tcagggattt gcgctaatgc atttcgctca gcgagttcgc
    22921 gccctggttg tgttgaatgg cgtggcgtta ttgccccaat ttgcctgtaa acaggggctt
    22981 gcgaacggtg aactggtgcg cctgtttgca ccgtggagcg gcatacccag attgttatat
    23041 gccttatttg cggggcgaaa ggggatgcct gccattgcgc gatattttat ggatgagtta
    23101 accacgcggc ttgccaacgg ggtctgaatc gctttttttg tatataatgc gtgtgaaatt
    23161 tcataccaca ggcgaaacga tcatgacgca ggatgaattg aaaaaagcag taggatgggc
    23221 ggcacttcag tatgttcagc ccggcaccat tgttggtgta ggtacaggtt ccaccgccgc
    23281 acactttatt gacgcgctcg gtacaatgaa aggccagatt gaaggggccg tttccagttc
    23341 agatgcttcc actgaaaaac tgaaaagcct cggcattcac gtttttgatc tcaacgaagt
    23401 cgacagcctt ggcatctacg ttgatggcgc agatgaaatc aacggccaca tgcaaatgat
    23461 caaaggcggc ggcgcggcgc tgacccgtga aaaaatcatt gcttcggttg cagaaaaatt
    23521 tatctgtatt gcagacgctt ccaagcaggt tgatattctg ggtaaattcc cgctgccagt
    23581 agaagttatc ccgatggcac gtagtgcagt ggcgcgccag ctggtgaaac tgggcggtcg
    23641 tccggaatac cgtcagggcg tggtgaccga taatggcaac gtgatcctcg acgtccacgg
    23701 catggaaatt cttgacccga tagcgatgga aaacgccata aatgcgattc ctggcgtggt
    23761 gactgttggc ttgtttgcta accgtggcgc ggacgttgcg ctgattggca cacctgacgg
    23821 tgtcaaaacc attgtgaaat gatctgacgg gggaacctcc cccgttaaaa aaattctctt
    23881 cattaaattt ggtgacatgt gtcacgcttt taccaggcaa ttgtcgattg ctctaaataa
    23941 atcctctaaa ccagcatatt catccaagaa ttacctttgc gtgatatttc ctcaacatcg
    24001 cgacgcaaac gttcatattg ccgcaatatt attttttgat atgttgaaag gcggatgcaa
    24061 atccgcacac aacatttcaa aagacaggat tgggtaaatg gcaaaggtat cgctggagaa
    24121 agacaagatt aagtttctgc tggtagaagg cgtgcaccaa aaggcgctgg aaagccttcg
    24181 tgcagctggt tacaccaaca tcgaatttca caaaggcgcg ctggatgatg aacaattaaa
    24241 agaatccatc cgcgatgccc acttcatcgg cctgcgatcc cgtacccatc tgactgaaga
    24301 cgtgatcaac gccgcagaaa aactggtcgc tattggctgt ttctgtatcg gaacaaacca
    24361 ggttgatctg gatgcggcgg caaagcgcgg gatcccggta tttaacgcac cgttctcaaa
    24421 tacgcgctct gttgcggagc tggtgattgg cgaactgctg ctgctattgc gcggcgtgcc
    24481 ggaagccaat gctaaagcgc accgtggcgt gtggaacaaa ctggcggcgg gttcttttga
    24541 agcgcgcggc aaaaagctgg gtatcatcgg ctacggtcat attggtacgc aattgggcat
    24601 tctggctgaa tcgctgggaa tgtatgttta cttttatgat attgaaaaca aactgccgct
    24661 gggcaacgcc actcaggtac agcatctttc tgacctgctg aatatgagcg atgtggtgag
    24721 tctgcatgta ccagagaatc cgtccaccaa aaatatgatg ggcgcgaaag agatttcgct
    24781 aatgaagccc ggctcgctgc tgattaatgc ctcgcgcggt actgtggtgg atattccggc
    24841 gctgtgtgat gcgctggcga gcaaacatct ggcgggggcg gcaatcgacg tattcccgac
    24901 ggaaccggcg accaatagcg atccatttac ctctccactg tgtgaattcg acaacgtcct
    24961 tctgacgcca cacattggcg gttcgactca ggaagcgcag gagaatatcg gcctggaagt
    25021 tgcgggtaaa ttgatcaagt attctgacaa tggctcaacg ctctctgcgg tgaacttccc
    25081 ggaagtctcg ctgccactgc atggtggtcg tcgtctgatg cacatccacg aaaaccgtcc
    25141 gggcgtgcta actgcgctga acaaaatctt cgccgagcag ggcgtcaaca tcgccgcgca
    25201 atatctacaa acttccgccc agatgggtta tgtagttatt gatattgaag ctgacgaaga
    25261 cgttgccgaa aaagcgctgc aggcaatgaa agctattccg ggtaccattc gcgcccgtct
    25321 gctgtactaa ttccccttct ctgaaaatta acgggcaggt cactgacttg cccgtttttt
    25381 tatcccttct ccacaccgtt gccgcgttgc gcattctact ttgcgagtct gctccagaaa
    25441 ataaatcaaa gtcagatcaa tgcgttgtgt ttatttaatg gggatttacg atggcgggac
    25501 agcatggggc tgtaacggta aagccctcac cgaagcgagg gcttaaagga gaagggttat
    25561 gatgcgactt gtcatcatac tgattgtact gttactgata agtttcggag cttattaaca
    25621 gtcagtctca ggggaggagt catcctccct tacccttact cactaaatta ggtcaaagaa
    25681 tcaacgatgt caatcagggc gatgcggggt gcatcgccct gaaccgaact ttttgatagc
    25741 cacttcatcg cccacgttta ctgccgtaat agatcctttt tcccaaaaat tatctctgtt
    25801 ttgcatgggg tttttcgagc acgctcgaag aaaagattta aaggtagatc aatgcgttgt
    25861 ggttatttaa agagagctta cgatagctgg gcagcatggg gctgtaacgg taaagccctc
    25921 accgaagcga gggcttgaag gagaagggtt atgatgcgac ttgccatcat actgattgta
    25981 ctgttactca taagtttccc agcttattaa cagtcagtca caggggagga gcaatcctcc
    26041 cttaccctta ctcactaaat taggttaaag aatcaacgat gtcaatcagg gcgatgcagg
    26101 tgtatcgccc ttaccactcc cagactttcg acggtgtaac caccgcagga agagggatat
    26161 cccactcttc aacggggagt ttttccacca actgacaatc atgcgcataa cccaccggtt
    26221 gcgttttata gtgctgccag ttttgtaagg tccgatcata aaaaccaccg cccattccca
    26281 ggcgctgacc gtactcatca aaggcgacca gcggtgtgat cagcacgtct aatcgggaaa
    26341 ggggtagcac gtcacgcaca tccagttttg gctcatggat tttcaacctg ttcatcacca
    26401 gttcgctttg cggatggtaa ttcaggaaca gcaaattacc ggcactaaag ggatgcaaaa
    26461 ctggaagata aacgcgctta ccggcgcgcc agagttgttc tatgagtggc tgggtgtcga
    26521 gttcgccatc aaaagagagg aatacagcga ccgtatgtgc catcaccacc gggggataag
    26581 tcatcatccg ggtagcggct tgttgaccca tttcttgctg ttgttccggc gttaacgcac
    26641 gacgacgttg ccgaatcatt ttgcggattt cttgtcggga taatgtcagt gggagttctg
    26701 gtagttgcgt catggctggt gccagataag aagggaatct ccgagatgcc gccgcaggct
    26761 gtaacccttg aacccttggt tcaaggtgaa tgtgtcgtcg cagttttaag gcttctcgga
    26821 cggaccgagc atgctcacca accgcggagc gccacattct tgtggtatga aatatcggct
    26881 caggggactg gcccgcttgc gaacatctca gagaaatttt gtcttcacgg ttactctacc
    26941 acagtaaacc gaaaagtgtc attcaaagtt ttggttagtt ttttcggtga tgcgaccttg
    27001 ttcaagtaac gcttgttcta tggtctgctg cagcatccga atacgctgtt ccatacttgc
    27061 cgcgtagtca cgagtctttg ctttttcttg cgctaactca tagctgatat tcaatgcggc
    27121 aatgaagacc aactgttcag tatttgtgac tctagtgcgt tctttcagat cttgcaaccg
    27181 ttggttcaga tcgtccgctg cctgattcaa cgcatccctt tggtcaggcg ggcagttcac
    27241 acgcagtgaa cggccaaaaa tttggatatc gacgggttgt gcagacatgc caccttcctg
    27301 ctgattgact gcgctgcctt cgtctttaga cccatggtct gcgaaggggc gacactatag
    27361 ctaccctgat gagaagagac aagccctttt ctggtccacc agggcccaaa gtggtagcat
    27421 atcatgaata ttcctccctt tgacgacgaa tgcttatgtc tatacagaac gaaatgcctg
    27481 gttacaacga aatgaaccag tatctgaacc aacaagggac gggtctgacc cctgctgaga
    27541 tgcatggttt aatcagtggg atgatatgtg gcggtaacga tgacagctca tggctaccgc
    27601 tacttcacga cctgacgaac gaaggcatgg ctttcggtca tgagctggca caggcactgc
    27661 gtaaaatgca ctctgccacc agcgatgccc tgcaggatga cggcttcctt tttcagcttt
    27721 atctgcctga tggcgatgat gtcagcgttt tcgatcgggc tgatgcattg gcaggttggg
    27781 tcaatcactt cctgcttggt cttggcgtta cgcaaccgaa gctggataaa gtgaccggcg
    27841 aaaccggtga agctatcgac gatctgcgta acattgcgca actgggttac gacgaagacg
    27901 aagatcagga agagcttgaa atgtcgcttg aagagatcat cgaatacgtt cgtgttgccg
    27961 cgctgttatg ccacgacacc tttactcatc cgcaaccgac cgcgccagaa gtacaaaaac
    28021 cgactctaca ctaaaaacaa aaaacgtaag gagagtgtta tgagtgagat atcccggcaa
    28081 gagtttcagc gtcgccgtca ggccctggtg gagcaaatgc aacccggcag cgccgcgctg
    28141 atttttgctg caccagaagt aacacgtagc gccgacagcg aataccccta tcgtcagaac
    28201 agtgacttct ggtacttcac cggctttaac gaaccggaag cggtgctggt gctgattaaa
    28261 agcgatgaca ctcataacca cagcgttctg tttaaccgcg ttcgcgacct gacggcggag
    28321 atctggtttg gccgtcgctt aggccaggat gccgcgccag agaaactggg cgttgaccgc
    28381 gcactggcat tcagcgaaat caatcagcaa ctttatcaac tacttaacgg cctggatgtg
    28441 gtttaccatg cccagggcga atatgcatat gctgatgaaa tcgtgaacag tgcgctggaa
    28501 aaactgcgta aaggttcgcg gcaaaatctc accgcaccgg caacgatgat cgactggcgt
    28561 cctgttgttc atgaaatgcg cctgttcaaa tcgccagaag agattgccgt actccgccgc
    28621 gcgggagaaa tcaccgccat ggcacataca cgggcgatgg aaaaatgccg tccgggaatg
    28681 ttcgagtacc atctggaagg cgaaattcac cacgaattta accgccacgg tgcgcgctat
    28741 ccgtcctata acaccattgt cggcagcggt gaaaacggct gcattctgca ctacaccgaa
    28801 aacgagagtg aactgcgcga cggtgacctg gtgttgattg acgcgggttg tgaatacaaa
    28861 ggttacgcgg gcgatattac ccgcaccttc ccggtcaacg gcaaattcac ccaggcccag
    28921 cgtgaaatct acgacattgt gctggagtct ctcgaaacca gcctgcgcct gtatcgtccg
    28981 ggaacttcca ttctggaagt cactggtgaa gtggtgcgca tcatggttag cggcctggta
    29041 aaactcggca tcctgaaagg tgatgttgat gaactgatcg ctcagaacgc ccatcgtcct
    29101 ttctttatgc atggccttag ccactggtta ggactggatg tccatgacgt tggcgtttat
    29161 ggtcaggatc gctcgcgcat tctggaaccg gggatggtac tgaccgtaga gcctggtctg
    29221 tatatcgcgc cggatgcgga tgtgccagaa caatatcgcg gtatcggcat tcgtattgaa
    29281 gacgacattg tgattaccga aaccggcaac gaaaacctca ccgccagcgt ggtgaaaaag
    29341 ccggaagaaa tcgaagcgtt gatggcagct gcgagaaagc aatgagcgta atcatcgtcg
    29401 gtggcggcat ggcgggcgcg acgctggcgc tggctatttc ccggttaagt cacggggcgc
    29461 tgccggtaca tttgattgaa gcgaccgcgc cagagtcaca tgctcatccg ggctttgatg
    29521 gacgagcgat agcgctggcg gcgggtacct gtcagcaact ggcgcgcatc ggcgtctggc
    29581 aatctctggc ggattacgca accgccatca ctaccgtgca tgtcagcgat cgtggtcacg
    29641 ctggatttgt caccctcgcc gcagaagatt accaactggc ggtgctggga caggttgtcg
    29701 aattgcacaa tgtcgggcaa cggctgtttg cattgctgcg taaagcacct ggcgtaacgc
    29761 tgcattgccc tgatcgcgtg gctaacgttg cccgtactca gagtcacgtt gaagtgacgc
    29821 tggagagtgg cgagacgctg acgggccgcg tgctggtagc agctgatggc acccattcag
    29881 cgttagccac cgcgtgcggc gttgactggc agcaggagcc ttacgaacaa ctggccgtga
    29941 ttgccaacgt tgcgacttcc gttgcgcacg aaggccgcgc ttttgaacgc ttcacgcaac
    30001 atggcccgct ggcgatgttg ccgatgtctg acggacgctg ttcgctggtc tggtgtcatc
    30061 cactggaacg gcgcgaagag gtgctgtcgt ggagcgacga gaagttttgc cgtgaactcc
    30121 agtcggcctt tggctggcga cttggaaaaa ttacccacgc cggtaaacgc agtgcttatc
    30181 cgctggcgtt aacccgcgcc gccagagcta ttacccatcg taccgtgctg gtgggcaatg
    30241 cggcgcaaac tctgcacccg attgccgggc aagggtttaa cctcggtatg cgagatgtca
    30301 tgagccttgc ggaaaccctg actcaggcgc atgagcgcgg agaagacatc ggtgattacg
    30361 gcatattgtg ccgctatcag cagcgtcgac agagcgatcg cgaagcaacc atcggcgtca
    30421 cggacagcct ggtacatctt tttgccaacc gttgggcacc gctggttgtc gggcgcaaca
    30481 tcggactgat gacgatggaa ttattcaccc cggcacgcga tgtgctggcg cagcgcaccc
    30541 tcggttgggt ggcgcgttga aggcggtttt aaggaataaa caatgcaaag tgttgatgta
    30601 gccattgttg gtggcggcat ggtggggctg gcggttgcct gcggcttaca ggggagcggc
    30661 ttacgcgttg ccgtactgga gcagcgcgta ccggaacctc tggcggcgga tgcaccacca
    30721 caactgcgcg tttcggctat caatgccgcc agcgaaaaat tactcacccg tcttggcgtc
    30781 tggcaggaca ttctctctcg tagggccagt tgttatcacg gtatggaagt gtgggacaaa
    30841 gacagttttg gtcacatttc gtttgacgat caaagcatgg gctatagcca tcttgggcat
    30901 atcgttgaaa actcagtgat tcactacgcg ctgtggaaca aagcgcagca gtcgtcagat
    30961 accactctgt tggcccccgc agaattacag caggtcgcct ggggagaaaa cgaaaccttc
    31021 ctgacgctga aagatggcag tatgttaacg gcgcgtctgg tgattggcgc ggacggcgct
    31081 aattcctggt tgcgcaacaa agccgatatt ccgctgactt tctgggatta tcagcatcac
    31141 gcgctggtag cgaccattcg cacggaagaa ccgcatgatg cggtggcgcg gcaggttttc
    31201 catggcgaag gcattctggc ctttttaccg cttagcgatc cgcatctttg ctcgattgtc
    31261 tggtcactgt cgccagagga agcgcagcgt atgcagcagg caggggaaga cgaatttaat
    31321 cgcgcgttaa atatcgcttt tgataatcgc ctgggcttat gcaaggttga gagcgcgcgt
    31381 caggtgttcc cactgacggg gcgttatgcg cgccagtttg ccgcgcaccg tctggcgctg
    31441 gtgggcgacg ccgcgcatac cattcacccg ctggcggggc agggggtaaa tctcggcttt
    31501 atggatgctg cagagctggt tgctgaactg aaacggttgc atcgtcaggg taaagacatc
    31561 gggcagtaca tttatctgcg tcgctatgag cgtagccgca agcacagtgc ggcgatgatg
    31621 ctggctggta tgcagggatt ccgcgatctg ttttccggta ccaatccggc gaaaaaactg
    31681 ctgcgtgata ttggtctgaa actggccgac acgcttcctg gcgttaaacc acaacttatc
    31741 cgccaggcaa tgggattaaa cgatttgcct gaatggctgc gttaaaaatt tctcttctgc
    31801 tgtttatttg atacccatca cactttcatc tcccggtttt ttcgccggga gattttcctc
    31861 atttgaaata aactaatttc acctccgttt tcgcattata ttttctaatg tcacgaattt
    31921 ttgatttagt gttttttgac attttttcag ctcttaatat tgtctt
//
LOCUS       DAFNBJ010000043        31728 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000043 DAFNBJ010000000
VERSION     DAFNBJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.043"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..213
                     /locus_tag="L2X49_001855"
     CDS             <1..213
                     /locus_tag="L2X49_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4796946.1"
                     /translation="RDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQIFRRDIRAS
                     SELTQAEAVKALGFLKQKASEQKVAA"
     gene            210..890
                     /locus_tag="L2X49_001856"
     CDS             210..890
                     /locus_tag="L2X49_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBN4796947.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWNKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEICTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            887..1045
                     /locus_tag="L2X49_001857"
     CDS             887..1045
                     /locus_tag="L2X49_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HBN4796948.1"
                     /translation="MTHPHDNIRVGAITFVYSITKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKQEDI"
     gene            1042..2106
                     /locus_tag="L2X49_001858"
     CDS             1042..2106
                     /locus_tag="L2X49_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HBN4796949.1"
                     /translation="MSQVGNHSFEFPASQGVQGGTVTLFLTIPGRSLARFLASDNYGH
                     TLERSQREINPNRVRKFLNYLTNADSRNESFIIPPLVGNCDSNIEFVPFGNTNVGIAR
                     IPLDAEIKLFDGQHRAAGIEIFCRSSPSTLMVPMMLTMNLPLKTRQQFFSDINNNVSK
                     PSATINMAYNGRDDIAQGMISFLTQHTVFADITDFEHNVVPLKSNMWVSFKALTDATS
                     KFARNGNQQLEMGYIESVWEAWITLTQIDSIRHGVHHATYKRDYIQFHGVMINAFGFA
                     VQQMMVNHSIAEITSMIEKLCATTSSAEREDFFLMDNWAGICTKASQEKLSVIANVAA
                     QKAAANRLIQAFTKGSLETT"
     gene            2260..2478
                     /locus_tag="L2X49_001859"
     CDS             2260..2478
                     /locus_tag="L2X49_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBN4796950.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPLDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            2526..2765
                     /locus_tag="L2X49_001860"
     CDS             2526..2765
                     /locus_tag="L2X49_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBN4796951.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRAIRRK"
     gene            2905..3141
                     /locus_tag="L2X49_001861"
     CDS             2905..3141
                     /locus_tag="L2X49_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBN4796952.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            3131..4273
                     /locus_tag="L2X49_001862"
     CDS             3131..4273
                     /locus_tag="L2X49_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HBN4796953.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFESVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWVIVDIKTA"
     gene            complement(4387..5637)
                     /gene="icd"
                     /locus_tag="L2X49_001863"
     CDS             complement(4387..5637)
                     /gene="icd"
                     /locus_tag="L2X49_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBN4796954.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            5809..6462
                     /gene="rluE"
                     /locus_tag="L2X49_001864"
     CDS             5809..6462
                     /gene="rluE"
                     /locus_tag="L2X49_001864"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBN4796955.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            6472..6933
                     /gene="nudJ"
                     /locus_tag="L2X49_001865"
     CDS             6472..6933
                     /gene="nudJ"
                     /locus_tag="L2X49_001865"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBN4796956.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6987..8093
                     /gene="mnmA"
                     /locus_tag="L2X49_001866"
     CDS             6987..8093
                     /gene="mnmA"
                     /locus_tag="L2X49_001866"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBN4796957.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            8129..8770
                     /gene="hflD"
                     /locus_tag="L2X49_001867"
     CDS             8129..8770
                     /gene="hflD"
                     /locus_tag="L2X49_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBN4796958.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8774..10144
                     /gene="purB"
                     /locus_tag="L2X49_001868"
     CDS             8774..10144
                     /gene="purB"
                     /locus_tag="L2X49_001868"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBN4796959.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            10313..10984
                     /gene="phoP"
                     /locus_tag="L2X49_001869"
     CDS             10313..10984
                     /gene="phoP"
                     /locus_tag="L2X49_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBN4796960.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHI
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10984..12444
                     /gene="phoQ"
                     /locus_tag="L2X49_001870"
     CDS             10984..12444
                     /gene="phoQ"
                     /locus_tag="L2X49_001870"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBN4796961.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            12520..13641
                     /gene="roxA"
                     /locus_tag="L2X49_001871"
                     /gene_synonym="ycfD"
     CDS             12520..13641
                     /gene="roxA"
                     /locus_tag="L2X49_001871"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBN4796962.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPSLD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(13690..14916)
                     /gene="pepT"
                     /locus_tag="L2X49_001872"
     CDS             complement(13690..14916)
                     /gene="pepT"
                     /locus_tag="L2X49_001872"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBN4796963.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVESYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            15166..16302
                     /gene="potA"
                     /locus_tag="L2X49_001873"
     CDS             15166..16302
                     /gene="potA"
                     /locus_tag="L2X49_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBN4796964.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            16286..17143
                     /gene="potB"
                     /locus_tag="L2X49_001874"
     CDS             16286..17143
                     /gene="potB"
                     /locus_tag="L2X49_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBN4796965.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            17140..17934
                     /gene="potC"
                     /locus_tag="L2X49_001875"
     CDS             17140..17934
                     /gene="potC"
                     /locus_tag="L2X49_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBN4796966.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLDIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17931..18977
                     /gene="potD"
                     /locus_tag="L2X49_001876"
     CDS             17931..18977
                     /gene="potD"
                     /locus_tag="L2X49_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBN4796967.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            19035..19496
                     /locus_tag="L2X49_001877"
     CDS             19035..19496
                     /locus_tag="L2X49_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HBN4796968.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            19493..20281
                     /locus_tag="L2X49_001878"
     CDS             19493..20281
                     /locus_tag="L2X49_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBN4796969.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(20420..21241)
                     /gene="cobB"
                     /locus_tag="L2X49_001879"
     CDS             complement(20420..21241)
                     /gene="cobB"
                     /locus_tag="L2X49_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBN4796970.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHECAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(21257..22168)
                     /gene="nagK"
                     /locus_tag="L2X49_001880"
     CDS             complement(21257..22168)
                     /gene="nagK"
                     /locus_tag="L2X49_001880"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBN4796971.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(22197..23441)
                     /gene="lolE"
                     /locus_tag="L2X49_001881"
     CDS             complement(22197..23441)
                     /gene="lolE"
                     /locus_tag="L2X49_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBN4796972.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23441..24142)
                     /gene="lolD"
                     /locus_tag="L2X49_001882"
     CDS             complement(23441..24142)
                     /gene="lolD"
                     /locus_tag="L2X49_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBN4796973.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(24135..25334)
                     /gene="lolC"
                     /locus_tag="L2X49_001883"
     CDS             complement(24135..25334)
                     /gene="lolC"
                     /locus_tag="L2X49_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBN4796974.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25596..26669
                     /locus_tag="L2X49_001884"
     CDS             25596..26669
                     /locus_tag="L2X49_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBN4796975.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            26797..30243
                     /gene="mfd"
                     /locus_tag="L2X49_001885"
     CDS             26797..30243
                     /gene="mfd"
                     /locus_tag="L2X49_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBN4796976.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVHEYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARILLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            30387..31349
                     /gene="ldtC"
                     /locus_tag="L2X49_001886"
     CDS             30387..31349
                     /gene="ldtC"
                     /locus_tag="L2X49_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBN4796977.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(31432..31689)
                     /gene="bhsA"
                     /locus_tag="L2X49_001887"
     CDS             complement(31432..31689)
                     /gene="bhsA"
                     /locus_tag="L2X49_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBN4796978.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         8008 a         8073 c         7922 g         7725 t
ORIGIN      
        1 cgcgacatca ctccggttaa cgatgaaacc atgcaggaga ttaacactct gctgattgcc
       61 ctggataaaa catgggatga cgacttattg ccgctctgtt cccagatatt tcgccgcgac
      121 attcgcgcat cgtcagaact gacacaggcc gaagcagtga aagctcttgg attcctgaaa
      181 cagaaagcct ctgagcagaa ggtggctgca tgacaccgga cattatcctg cagcgtaccg
      241 ggatcgacgt gagagctgtc gaacagggag atgatgcgtg gaacaaatta cgactcggcg
      301 tcatcacggc ttcagaagtt cacaatgtga tagcaaaacc ccgctccggt aaaaagtggc
      361 ctgacatgaa aatgtcctac tttcacaccc tgctggctga gatttgcacc ggtgtggctc
      421 cggaagttaa cgctaaggcg ctggcctggg gaaaacagta cgagaatgac gccagagccc
      481 tgtttgagtt tacttccggc gtgaatgtta ctgaatcccc gatcatctat cgcgacgaaa
      541 gtatgcgcac cgcctgctct cccgatggtt tatgcagtga cggcaacggc cttgagctga
      601 aatgcccgtt tacctcccgg gatttcatga agttccggct cggtggtttc gaggccataa
      661 agtcggctta catggcccag gtgcagtaca gcatgtgggt gacacgaaaa gatgcctggt
      721 actttgccaa ctatgacccg cgtatgaagc gtgaaggact gcattatgtc gtggttgagc
      781 gggatgaaaa gtacatggcg agttttgacg agatggtgcc ggagttcatc gaaaaaatgg
      841 acgaggcact ggctgaaatt ggttttgtat ttggggagca atggcgatga cgcatcctca
      901 cgataatatc cgggtaggcg cgatcacttt cgtctactcc attacaaagc gaggctgggt
      961 atttcccggc ctttctgtta tcagaaatcc actgaaagca cagcggctgg ctgagaagat
     1021 aaataataaa caggaggata tatgagtcag gttggtaatc attcattcga atttccggca
     1081 tcgcaaggtg tacagggtgg tactgttaca ctcttcctta ccataccagg aagatcgctg
     1141 gctcgtttcc tcgcttcaga taattacggc catacactgg aacgctctca gcgagaaatt
     1201 aatccaaatc gagtacgaaa atttttaaat tatctcacta acgcagactc aagaaatgag
     1261 tcttttatca ttccccctct cgtaggtaac tgtgattcga atatagaatt tgtaccgttt
     1321 ggcaacacaa atgttggtat agccagaatt cccctcgacg ccgaaataaa actttttgat
     1381 ggccaacatc gtgcagctgg cattgagata ttttgccgaa gttccccatc aacgctcatg
     1441 gttcccatga tgcttacaat gaatctgccg ctaaaaaccc ggcagcagtt cttttcggac
     1501 ataaataaca acgtttctaa gccatcagcg accatcaata tggcgtataa cggccgggat
     1561 gatattgctc agggaatgat atccttcctg acccaacata ctgtatttgc cgatataacc
     1621 gattttgaac acaacgtagt gccattaaaa agtaatatgt gggtgagttt caaggcactc
     1681 actgatgcaa cgtcaaagtt tgctaggaac ggcaatcaac aacttgaaat gggatatata
     1741 gaatctgtct gggaggcatg gattacacta actcagattg actcaatccg acatggtgta
     1801 caccacgcta cgtacaagcg cgattatatt cagttccatg gagtaatgat taacgctttc
     1861 ggttttgcgg ttcaacagat gatggttaat cattccatcg cagaaataac ttctatgatc
     1921 gaaaaactct gtgcaactac cagctctgca gaaagagagg atttttttct gatggataac
     1981 tgggcgggga tctgcacgaa agccagccag gaaaaactat cggttattgc caatgtggca
     2041 gcgcagaaag cagcagcaaa cagactgata caagctttta ccaaaggaag tctggaaaca
     2101 acttaatgaa tcaacattgt ctcatatcag catgctgtac ggcgtcttta aggaacggtg
     2161 agcatgaaaa acaaaatcat catggagcta caggctcctt ttttattatt cgcattcacc
     2221 ctcaagcgta ttaaccaaca attcagggat taatgaaaga tggcagacat cattgattca
     2281 gcatcagaaa ttgaagaatt acagcgcaac acagcaataa aaatgcgccg cctgaaccac
     2341 caggctatat ctgccactca ttgttgtgag tgtggcgatc cgctagatga acgaagacgc
     2401 ctggccgttc agggttgtcg gacttgtgca agttgccagg aggagatcga acttaagaac
     2461 aaacaatggg gactgtgatg gcctcaaagc agcaaatttc aacatcgtcc aactgaggtg
     2521 taaaaatgtt cagaatcatt tttcctaaca cctggtacgt cgaccaccac ggcactccct
     2581 gcaaaatcct gcgttctacc cacaacaaag ttcactacat ccgaaaaggc agaacatgta
     2641 tcgccagcat gttccgcttt aatcatgact ttgaacctgt gaataaagct gatgcagatc
     2701 ggatagcaga agagatcgaa acggcagaac acattaagaa gttacgtgcc atacgcagga
     2761 aatagaaaaa ttgataaatt caatactgca tttctcagca ttaaatttat ctctatgacc
     2821 agtcaagaga tgtacctgcc atgagcttaa tatcatgtca gatatatcgg tcacaaactc
     2881 cctcagcagc taagaggagg acaaatgtct cgactaatca ctttacagga ctgggctaaa
     2941 gaagaatttg gggacttagc accaagtgag cgagttctga aaaaatacgc gcaagggaaa
     3001 atgatggccc cacccgctat aaaagttggt cgctactgga tgattgaccg aaattcccgt
     3061 tttgtaggaa cgctggcaga accgcaactc ccaataaacg caaacccaaa actccaacgg
     3121 ataatcgctg atggctgcta gaccccgatc tcacaaaatc tctataccca atttatattg
     3181 caaattagat aagcgaaccg gaaaggtata ttggcaatac aaacatccac tatccggtcg
     3241 ttttcatagc ttaggaactg atgagaatga agcaaaacaa gttgctactg aagcaaatac
     3301 cattattgct gaacaacgta ccagacaaat attaagcgtc aatgagcgtc tggaaagaat
     3361 gaaaggcagg cgctcagaca ttacggtgac agaatggctt gataaatata tttctatcca
     3421 ggaggacagg ctgcaacata atgaactaag acccaactcc tatcggcaaa aaggcaaacc
     3481 cattcgtctt ttccgtgagc attgtggaat gcaacacctc aaggatatta ccgcacttga
     3541 tattgccgaa ataattgatg ctgtaaaggc tgaaggtcat aacaggatgg cgcaagtcgt
     3601 gagaatggtg ttgatcgacg tcttcaaaga agcacaacac gcaggacatg ttccgccagg
     3661 atttaaccca gcgcaggcaa caaaacaacc gcgaaatcga gtaaaccgcc aaagattgtc
     3721 actgcccgaa tggcaggcaa tatttgaaag cgtaagcaga cggcagccct atttaaaatg
     3781 cggcatgcta cttgctcttg ttactggaca acgtttaggc gatatctgca atttgaaatt
     3841 ctctgatata tgggacgaca tgttgcacat tactcaggaa aaaaccggtt caaaacttgc
     3901 tattccgctt aacctgaaat gcgatgctct gaatattacc cttcgtgaag ttatatctca
     3961 gtgcagggat gctgttgtta gtaaatatct ggtccattac cgtcacacta cctctcaagc
     4021 aaacagagga gaccaggttt ctgcaaatac tctgacaacg gcttttaaaa aggccaggga
     4081 aaaatgtggc ataaaatggg agcaaggaac tgcgcccaca tttcatgagc agcgatctct
     4141 gtcagaacgg ttatatcggg aacagggtct ggatacgcaa aagttgttag gccataaatc
     4201 cagaaaaatg accgaccgat acaatgatga tcgtggtaaa gactgggtta tcgtagatat
     4261 caaaacagca tagaaaatag ccagttttgg ggaagggttt tggggaaagt tttggggaag
     4321 attttacatc atcataaaac aacgggcgta taacacgccc gtttcaatat ttaacacatg
     4381 tagagattac atgttcttga tgatcgcatc accaaactct gaacatttca acagtttagc
     4441 gccttccatc agacgttcga agtcataggt tacggtcttc gcattgattg cgccttccat
     4501 acctttaaca atcaggtctg cggcttcggt ccaacccatg tggcgtaaca tcatctcagc
     4561 agagagaata atagagcctg ggtttacttt gtcctgaccg gcatatttcg gcgcagtacc
     4621 gtgggtggct tcaaacaggg cgcattcgtc accgatgttt gcacctgggg cgataccgat
     4681 accgccaacc tgcgctgcca gggcgtcaga aatgtagtca ccgttcaggt tcatacaggc
     4741 gataacatca tattcagccg gacgcagcag gatctgttgc aggaatgcat cagcaatcac
     4801 gtctttaatg acgatctctt tgccggtgtt cgggttttta actttcagcc acgggccgcc
     4861 gtcgatcagt tcaccgccaa actcttcacg cgccagctgg tagccccagt ctttaaacgc
     4921 tccttcggtg aacttcatga tgttgccttt gtgcaccaga gtcacagagt cacgatcgtt
     4981 agcaattgcg tattcgatcg ctgcacgaac cagacgtttg gtgccttctt ccgaacacgg
     5041 cttaataccg ataccacaat gttccgggaa gcgaattttc ttcaccccca tctcttcacg
     5101 caggaattta atcactttct cggcgtcggc agagtctgct ttccattcga tacccgcata
     5161 aatgtcttcc gagttttcac ggaagataac catatcggtc agttcagggt gtttaaccgg
     5221 gcttggagtg ccctgatagt aacgtaccgg acgcaggcag atgtagagat ccagttcctg
     5281 gcgcagggca acgttcagag agcgaatacc gccaccaaca ggagtggtca gcggaccttt
     5341 aatggcaacg cgatattcac gaatcagatc aagggtttca gcaggcagcc agacatcctg
     5401 accataaacc tgtgtggatt tttcaccggt gtaaatttcc atccaggaga ttttacgctc
     5461 gcctttatag gctttctcga ctgcagcgtc gaccactttc agcatggctg gggttacatc
     5521 tacaccgatt ccatcacctt caatgtaagg gataatcgga ttttcaggaa cgttgagttt
     5581 gccgttttgc agggtgatct tcttgccttg tgccggaaca actactttac tttccattca
     5641 cctctccttc gagcgctact ggtttgctcg tctgccacca cgttgcatat gcgtttgcgt
     5701 cctgcgatac ggatgcttta gagcaatttt ttgttaatga tttgtaattg gcttgtcaat
     5761 actaccctat tgtttgccgt catgaaagat gcgcgttatt aggctataat gcggcaattc
     5821 ataatctctg aaaataccat gcaaaaaact tcttttagaa atcaccaggt taagcgattc
     5881 agctcgcaac gttctaccag gcgtaaacct gaaaaccagc ccacgcgtgt gatcctgttc
     5941 aataaaccct acgatgttct tccgcagttc accgatgaag ccggacgcaa aacattaaaa
     6001 gaattcatcc cggttcaggg cgtttatgca gcaggtcgcc ttgaccgcga tagcgaaggg
     6061 ttgctggtgc tgaccaataa cggcgcgttg caggcgcgtt taacccagcc gggtaaacgc
     6121 accggaaaaa tctattatgt gcaggtggaa ggtattccca cacaagacgc acttgaagcc
     6181 ttgcgcaatg gcgtaacctt aaatgatggc cctaccctgc ccgcaggcgc ggagctggtt
     6241 gacgaacccg cgtggttatg gccgcggaat ccaccaattc gtgaacgcaa aagtattccc
     6301 accagctggc tgaagatcac cttatatgaa ggacgtaatc gccaggtgcg ccgcatgacc
     6361 gcccatgttg gtttccccac gctgcgactg attcgctatg cgatgggaga ttactctttg
     6421 gataatcttg ccaatggcga atggcgagaa gtgacagatt aaggatgcaa aatgtttaaa
     6481 ccgcacgtta ccgttgcttg cgtggtgcac gcagaaggca aatttttagt cgttgaagag
     6541 acgattaatg gtaaagcgtt atggaaccaa cctgccgggc atctggaagc cgatgaaacc
     6601 ttagtggaag ccgccgcccg tgaactgtgg gaagaaaccg gcatcagcgc gcagccgcaa
     6661 cactttattc gtatgcatca gtggattgcg ccagataaaa cgccgttttt gcgtttcctc
     6721 tttgccattg agcttgagca aatatgcccg acccagcctc atgacagcga tatcgactgc
     6781 tgccgttggg tcagcgccga agaaatttta caggcgtcaa atcttcgttc gccgctggtg
     6841 gcggaaagta ttcgttgtta tcaaagcggg caacgttatc cgctggagat gattggcgat
     6901 tttaactggc cttttacaaa gggtgtcatc taaaaggctg cgtgatagaa tacgccgcct
     6961 tgaagttcaa tgtcgtgagt gatccaatgt ctgaaaccgc aaaaaaagta atcgtcggca
     7021 tgtccggcgg tgtcgattcc tccgtttctg cctggctgtt gcaacaacag ggatatcagg
     7081 tcgaaggcct gtttatgaag aactgggaag aagacgacgg tgaggaatat tgcacagcgg
     7141 cagcggatct ggctgatgcc caggctgtct gcgacaagct cggcattgaa ctgcacaccg
     7201 ttaactttgc tgccgagtac tgggacaacg tcttcgaact gttccttgcc gaatataaag
     7261 ccggtcgcac gccgaatccg gatattctgt gcaacaaaga gatcaaattt aaagccttcc
     7321 tcgaatttgc cgccgaagat ttaggtgccg attatatcgc taccggtcat tacgtacgtc
     7381 gggccgatgt cgatggcaag agccgcctgc tgcgtggtct ggacagcaat aaagaccaga
     7441 gctacttcct ttatacgctc agccatgagc agattgcgca aagcctgttc ccggtcggcg
     7501 aactggaaaa accgcaggtg cgtaagattg ctgaagatct tggtctggtc accgcgaaga
     7561 aaaaagactc taccggcatt tgcttcattg gcgaacgtaa attccgcgag ttcctgggcc
     7621 gttatctccc ggcgcaaccg ggcaaaatca ttaccgtcga tggcgatgaa attggtgagc
     7681 accaggggct gatgtatcac actctcggtc agcgtaaagg tctgggtatc ggtggcacca
     7741 aagaaggtac cgaagaaccg tggtatgtgg tggacaaaga cgtcgaaaac aacattctgg
     7801 ttgtcgctca gggccatgaa cacccgcggc tgatgtctgt cgggttgatt gcccagcagt
     7861 tgcactgggt cgatcgcgaa ccattcaccg gcactatgcg ttgcacggta aaaacccgct
     7921 atcgccagac cgatatccct tgcaccgtca aggcgctgga cgatgatcgc attgaagtga
     7981 ttttcgatga accggttgcc gccgtgacgc cgggccagtc tgccgtcttc tataacggtg
     8041 aagtgtgcct cggtggcggt attattgagc agcgtctgcc gctgccggtc tgattattat
     8101 ctttacttaa aacaaggaag cagtgaacgt ggcaaagaat tactatgaca tcaccctcgc
     8161 cctggccggt atttgtcagt cggcacgcct ggtgcaacaa ctcgctcacc aggggcattg
     8221 tgatgccgat gcgctacacg tctcactcaa cagtattatt gatatgaacc ccagctcgac
     8281 gctggcggtt tttggcggta gcgaagccaa cctgcgcgtc gggctggaaa ccctgctcgg
     8341 cgtgctcaat gccagcagtc gccagggctt aaacgccgaa ttaacccgct acacactcag
     8401 cttgatggtg cttgagcgca aactctcctc agcgaaaggc gcgctcgaca ctctgggcaa
     8461 ccggatcaac ggcctgcaac gccagctcga acacttcgat ttacagtccg aaacgctgat
     8521 gagcgcgatg gctgctatct atgttgatgt gattagcccg cttggcccgc gcattcaggt
     8581 caccggttcc cctgctgtac tgcaaagccc acaagtgcag gcgaaagttc gcgcaaccct
     8641 gctggcaggc attcgcgccg ccgtgctctg gcaccaggtc ggcggcggac gtctgcaact
     8701 gatgttttct cgtaatcgcc tgaccactca ggcaaaacaa attcttgctc atttaacccc
     8761 ggagttgtga tctatggaat tatcctcact gaccgccgtt tcccctgtcg atggacgcta
     8821 cggcgataaa gtcagcgcgc tgcgcgggat tttcagcgaa tatggtttgc tgaaattccg
     8881 tgtacaagtt gaagtacgtt ggctgcaaaa actggccgcg cacgcagcga tcaaggaagt
     8941 tcctgctttt gctgccgacg caatcggtta ccttgatgca atcgtcgcca gtttcagcga
     9001 agaagatgcg gcgcgcatca aaactatcga gcgtaccact aaccacgacg ttaaagcggt
     9061 tgagtatttc ctgaaagaaa aagtggcgga gatcccggaa ctgcacgcgg tttctgaatt
     9121 catccacttt gcctgtactt cggaagatat caataacctc tcccacgcat taatgctgaa
     9181 aaccgcgcgt gatgaagtga tcctgccata ctggcgtcaa ctgattgatg gcattaaaga
     9241 tctcgccgtt cagtatcgcg atatcccgct gctgtctcgt acccacggtc agccagccac
     9301 gccgtcaacc atcggtaaag agatggcaaa cgtcgcctac cgtatggagc gccagtaccg
     9361 ccagcttaac caggtggaga tcctcggcaa aatcaacggc gcggtcggta actataacgc
     9421 ccacatcgcc gcttacccgg aagttgactg gcatcagttc agcgaagagt tcgtcacctc
     9481 gctgggtatt caatggaacc cgtacactac ccagattgaa ccgcacgact acattgccga
     9541 actgtttgat tgcgttgcgc gcttcaacac cattctgatc gactttgacc gtgacgtctg
     9601 gggttatatc gcccttaacc acttcaaaca gaaaaccatt gctggtgaga ttggttcttc
     9661 caccatgccg cataaagtta acccgatcga cttcgaaaac tccgaaggga atctgggcct
     9721 ttccaacgcg gtattgcagc atctggcaag caaactgccg gtttcccgct ggcagcgtga
     9781 cctgaccgac tccaccgtgc tgcgtaacct cggcgtgggt atcggttatg cgctgattgc
     9841 gtatcaatcc accctgaaag gcgtgagcaa actggaagtg aaccgtgacc atctgctgga
     9901 tgaactggat cacaactggg aagtgctggc tgagccaatc cagacagtta tgcgtcgcta
     9961 tggcatcgaa aaaccgtacg agaagctgaa agagctgact cgcggtaagc gcgttgacgc
    10021 cgaaggcatg aagcagttta tcgacggtct ggcgttgcca gaagaagaga aagcccgcct
    10081 gaaagcgatg acgccggcta actatattgg tcgagctatc acgatggttg atgagctgaa
    10141 ataaacctcg tatcagtgcc ggatggcgat gctgtccggc ctgcttatta agactatccg
    10201 ctttttattt tttcacttta cctcccctcc ccgctggttt atttaatgtt tacccccata
    10261 accacataat cgcgttacac tattttaata attaagacag ggagaaataa aaatgcgcgt
    10321 actggttgtt gaagacaatg cgttgttacg tcaccacctt aaagttcaga ttcaggatgc
    10381 tggtcatcag gtcgatgacg cagaagatgc caaagaagcc gattattatc tcaatgaaca
    10441 tataccggat attgcgattg tcgatctcgg attgccagac gaggacggtc tgtcactgat
    10501 tcgccgctgg cgtagcaacg atgtttcact gccgattctg gtattaaccg cccgtgaaag
    10561 ctggcaggac aaagtcgaag tattaagtgc cggtgctgat gattatgtga ctaaaccgtt
    10621 tcatattgaa gaggtgatgg cgcgaatgca ggcattaatg cggcgtaata gcggtctggc
    10681 ttcacaggtc atttcgctcc ccccgtttca ggttgatctc tctcgccgtg aattatctat
    10741 taatgacgaa gtgatcaaac tgaccgcgtt cgaatacacc attatggaaa cgttgatacg
    10801 caataatggc aaagtggtga gcaaagattc gttaatgcta caactctatc cggatgcgga
    10861 gctgcgggaa agccatacca ttgatgtact gatgggacgt ctgcgcaaaa aaattcaggc
    10921 acaatatccg caagaagtga ttaccaccgt tcgcggccag ggctatctgt tcgaattgcg
    10981 ctgatgaaaa aattactgcg tctttttttc ccgctctcgc tgcgggtacg ttttctgttg
    11041 gcaacggcag cggtagtact ggtgctttcg cttgcctacg gaatggtcgc gctgatcggt
    11101 tatagcgtca gtttcgataa aactacgttt cggctgttac gtggcgaaag caatctgttc
    11161 tatacccttg cgaagtggga aaacaataag ttgcatgtcg agttaccaga aaatatcgac
    11221 aagcaaagcc ccaccatgac gctaatttat gatgagaacg ggcagctttt atgggcgcaa
    11281 cgtgacgtgc cctggctgat gaagatgatc cagcctgact ggctgaaatc gaatggtttt
    11341 catgaaattg aagcggatgt taacgatacc agcctcttgc tgagtggaga tcattcgata
    11401 cagcaacagt tgcaggaagt gcgggaagat gacgacgacg cggagatgac ccactcggtg
    11461 gcggtaaacg tctacccggc aacatcgcga atgccaaagt taaccattgt ggtggtggat
    11521 accattccgg tggagctaaa aagttcctat atggtctgga gctggtttat ctatgtgctc
    11581 tcagccaatc tgctgttagt gatcccgctg ctgtgggtcg ccgcctggtg gagtttacgc
    11641 cccatcgaag ccctggcaaa agaagtccgc gaactggaag aacataaccg cgaattgctc
    11701 aatccagcca caacgcgaga actgaccagt ctggtacgaa acctgaaccg attgttaaaa
    11761 agtgaacgcg aacgttacga caaataccgt acgacgctca ccgacctgac ccatagtctg
    11821 aaaacgccac tggcggtgct gcaaagtacg ctgcgttctc tgcgtagtga aaagatgagc
    11881 gtcagtgatg ctgaaccggt aatgctggag caaatcagcc gcatttcaca gcaaattggc
    11941 tactacctgc atcgtgccag tatgcgcggc gggacattgc ttagccgcga gctgcatccg
    12001 gtcgccccac tgctggacaa tctcacctcg gcgctgaaca aagtgtatca acgcaaaggg
    12061 gtcaatatct ctctcgatat ttcgccagag atcagctttg tcggtgagca gaacgatttt
    12121 gtcgaggtga tgggcaatgt gctggataat gcctgtaaat attgcctcga gtttgtcgaa
    12181 atttctgcaa ggcaaaccga cgagcatctc tatattgtgg tcgaggatga tggacccggt
    12241 attccattaa gcaagcgaga ggtcattttc gaccgtggtc aacgggttga tactttacgc
    12301 cctgggcaag gtgtagggct ggcggtagcc cgcgaaatca ccgagcaata tgagggtaaa
    12361 atcgtcgccg gagagagcat gctgggcggt gcgcggatgg aggtgatttt tggtcgccag
    12421 cattctgcgc cgaaagatga ataaatatgt ccatacttca cgcattacgt taagcatccg
    12481 ttataatcgg ttgcagatac cagcctgtgg atgcttaaca tggaatacca actcactctt
    12541 aactggcccg attttcttga acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc
    12601 tttaataatt ttattgaccc gatctctcca gacgagttgg cgggtctggc gatggaaagc
    12661 gaagtcgaca gtcgactggt cagtcaccag gatggcaaat ggcaggtcag ccacggtccg
    12721 ttcgaaagct acgatcatct cggtgaaact aactggtcat tgttagtgca agcagtaaat
    12781 cactggcatg agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt
    12841 attgatgatc tgatgatctc tttttctgta cctggcggcg gcgtcggccc gcatctcgat
    12901 cagtacgacg tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag
    12961 ctgcaaatga aacagcactg cccacacccg gatctgttac aggtcgatcc gttcgaagcc
    13021 atcatcgatg aagagctgga gcctggcgat attctttata ttccgccagg attcccgcat
    13081 gaaggctacg cgctggaaaa tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg
    13141 cgggaattaa ttagcggatt tgccgattat gtgctgcaac gtgaactggg cggcaactac
    13201 tacagcgatc cggatgttcc acctcgcgct catcctgcgg acgttctgcc gcaagaaatg
    13261 gataaactgc gtgagatgat gctcgaattg atcaaccagc cggaacactt taagcaatgg
    13321 tttggcgagt ttatatccca gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct
    13381 tatcagccag atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt
    13441 ggtctgcgcg tattgcgcat tggcgacgac gtgtatgcca atggtgagaa gatcgattcc
    13501 ccgcaccgtc cgtcactgga tgcactcgcc agcaacattg cgctgaccgc ggagaatttt
    13561 ggcgatgcgc tggaagatcc gtcattcctc gcgatgctcg cggcgctggt caatagcgga
    13621 tactggttct tcgaagggta agtttggatt taagccggat gcggcatcac atgccgcatc
    13681 ccttttcgct tacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc
    13741 tttttccata ccttccagag tcacaaactc atgcttacca tgatagttgt aaccgccagt
    13801 gaacaggttc gggcacggta atcccataaa cgacaactgc gcgccgtcgg taccgccgcg
    13861 gatcggtttc agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag
    13921 aatatgcgga tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccag
    13981 ttcaatgtag caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt
    14041 acgtttacgc gcttcaaact gtttacggtc gaaatcacgg atgatgtagt gcatatcggc
    14101 tcggtcaacg gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat
    14161 ttccgggctt tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt
    14221 taccatcact cctttcgccg tgcccggatg aacattgtta ccgacaattt tgatattgac
    14281 cgatgcggcg ttgaagtttt caaactccag ttcgcctacg ccaccaccgt caacagtgta
    14341 agcccagcgg gcatcgaagg catcaacatc aaaatgtttc gcccctttgc ccacttcttc
    14401 atccggggta aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc
    14461 cagcgcggtc atgatttctg caataccggc tttgtcatcg gcacctaaca aggttttacc
    14521 atcggtggta atcaacgtct gacccagtag ctgatgcagc accgggaaca taaccggtga
    14581 taaaacttca tcgccgatac ccagcgcaat atcgccaccg cgatagcttt caacaatttg
    14641 cggattaaca tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat
    14701 cgccgggata tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact
    14761 taaggtcaca ttgataagcc ccatttcttc gagctgctct ttcagcagat gcaataactt
    14821 ccattggcct tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga
    14881 cacgtagttc aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac
    14941 aacattatta agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag
    15001 cgggaatatt tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa
    15061 agattggcga ttcaagcggt ttgccgtaga attaccgccc tcattaagag tcaccaaggt
    15121 ggttaaccac aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa
    15181 aaattgaata aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa
    15241 tgctttgatg gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc
    15301 ctcacgctgc ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt
    15361 ctggaaactg ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg
    15421 gcggaaaacc gctatgtgaa cactgttttc caaagctacg cacttttccc ccacatgacc
    15481 gtgttcgaaa atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg
    15541 ccccgcgtga tggaagccct gcggatggtg caactggaaa ccttcgctca acgtaaaccg
    15601 catcagctct ctggtggtca acagcaacgc gtcgccattg ctcgcgcggt ggttaacaag
    15661 cctcgtctgt tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa
    15721 atgcagaacg agctgaaagc gttacagcgt aagcttggta ttaccttcgt ctttgtcact
    15781 cacgatcagg aagaagcgct caccatgtca gacaggattg tggtgatgcg cgagggtcgt
    15841 attgagcaag atggtacacc gcgcgaaatt tacgaagagc cgaaaaacct gtttgttgcc
    15901 ggcttcattg gcgaaatcaa tatgtttaac gccactgtca tcgaacgtct agacgagcag
    15961 cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa
    16021 ccggggcaaa aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac
    16081 gacgacaacc acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg
    16141 acgctggagt cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc
    16201 aatgaagacg accctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta
    16261 gaaagctggg aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat
    16321 tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg
    16381 caccagcttt ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cgctggataa
    16441 ctacacgcgt ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc
    16501 gctgatcgcc accctcgcct gcctggtgct gggctacccg tttgcctggt ttctggcgaa
    16561 gctaccacac aaggtgcgtc cgctgctgct gtttctgctg attgttccct tctggaccaa
    16621 ctcactgatt cgcatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga
    16681 gtttttgctc tggctgggcg ttatcgacac accaattcgt atcatgttca cgcccagtgc
    16741 ggtgattatc ggtctggttt acattctgct accgtttatg gtgatgccgc tgtactccag
    16801 tatcgaaaaa ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt
    16861 acagactttt atccgtatca ttattccact gacgatgccg ggaattattg ccggatgtct
    16921 gctggtgatg ctgccagcga tgggcctgtt ctatgtatcc gacctgatgg gcggtgcgaa
    16981 aaacctgctg atcggtaacg tcatcaaggt ccagttcctc aatattcgtg actggccgtt
    17041 tggtgcagcc accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg
    17101 gcgcgcttct cgtctgctga ataaaaaggt ggaactcgaa tgatcggtcg actgcttcgc
    17161 ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg
    17221 aactccttta acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat
    17281 agcctgctga tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg
    17341 gtgttttcgg cgacatttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt
    17401 tatcgctttc gtggtaagcc gttcgttagc ggaatgctgt ttgtggtgat gatgtcacca
    17461 gatatcgtga tggcgatttc tctgctggtg ctgtttatgc tgctggatat tcagcttggc
    17521 ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc cgtttgtggt ggtgacggtg
    17581 tattcgcgcc tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc
    17641 agtgaattta ccattctgcg aaaaatcatt ctgccactgg caatgcctgc ggtggcggcg
    17701 ggctgggtgt taagctttac cctgtcgatg gacgatgtgg tagtttcttc gttcgtcacc
    17761 ggaccaagtt atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg
    17821 ccggaagtta acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc
    17881 agccagctta ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat
    17941 ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg
    18001 atgacaacaa cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg
    18061 aacagttcac caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa
    18121 ccatgtatgc caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa
    18181 cctattacgt cgataaaatg cgtaaagaag ggatgatcca gaagatcgac aagtcgaagt
    18241 taacgaactt cagcaatctc gatccagaca tgctcaataa gccgtttgac ccgaataacg
    18301 attactccat tccgtatatc tggggtgcga cggcgattgg tgttaacggt gatgcggtgg
    18361 atccgaaatc tgtcaccagc tgggccgatc tgtggaagcc tgagtacaaa ggcagcctgc
    18421 tgttgaccga cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctactccg
    18481 gtaacaccac cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc
    18541 caaacgtggc agcctttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta
    18601 acctcggcat gatctggaac ggttctgctt tcgttgcacg ccaggcgggt acgccaattg
    18661 acgtggtatg gccgaaagaa ggcggcattt tctggatgga cagcctggcg atcccggcaa
    18721 atgccaaaaa caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg
    18781 caaaacaggt tgccgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt
    18841 taagcccgga agtggcgaac gataaaacgc tctacccgga tgctgaaacc attaaaaatg
    18901 gtgaatggca gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc
    18961 tgaaagcagg acgttaattc agcaagcggg cggtggcatt accgcccgtt ttttatttag
    19021 caaggatgtg gctaatgagt caggacagta aagtgttatc ccgcgctttt ctcggtatcg
    19081 gtctgatatt actcgttatt tctgcaatca tcttttatta ccattttact tttaccaaaa
    19141 gcgcggttca tacagaagga atcattgttg atgccgtttg gtacaacaat cactcaaacg
    19201 acgtcgatga taatggttcc tggtatccag tggttgcctt tcgccctacg ccagattaca
    19261 cgctaatttt caattcgaat atcggtagtg atttttatga agatagcgaa ggtgacagag
    19321 tcaatgtcta ttatccgcca gggcatccag aacaagccga aataaacaat ccctgggtta
    19381 atttttttaa atggggcttt gttggcatag caggcattat tttcggtagt gtcggtctga
    19441 taatcacgct cccctccacc aaaaaatctc gcaggaaacg gaagtcccgc ccatgaaaaa
    19501 attcattata ctgctcagtt tactgatact cctgcctctg gtagcaacca gtaaaccatt
    19561 gattccaata atgaagacac tttttacgga tgttaccggt actgtaccgg atgctgaaga
    19621 gatcgcacgt aaagccgagc tttttcgcca acaaacggga attgcaccgt ttattgtgat
    19681 tttacccgac attaataatg aagccagtct cagacaaaat ggtaaagcga tgctggcgca
    19741 tgcgtcatct tcattgagta atgtaaaagg aagtgttctg ttactattta ctacccgcga
    19801 accacggtta attatgatca ccaacggcca ggttgaaagt ggtctggacg ataaacatct
    19861 cggccttctg atagaaaatc acacgctggc ttatttaaat gcagatctct ggtatcaggg
    19921 aatcaataat gcattggctg ttctacaagc acagatatta aaacaaccga cgccgccact
    19981 gacgtattat ccgcatccag gacaacagca tgaaaatgct ccccccggca gcaccaacac
    20041 attaggtttt atcgcctggg ctgcgacgtt tatcttattt tcctggatct tttgctacac
    20101 gactcgtttt atttatgcgt taaaatttgc agtggcgatg actattgcca atatgggtta
    20161 tcaggcttta tgtttgtata tcgataatag ctttgcgatt accagaatat cgccactctg
    20221 ggcagggcta attggtgtct gtacgtttat tgccgcattg ttattgacga gtaaacgata
    20281 aaagaggccg gaaaagtgcg cgtgtattat gccggatggg gcgcgaacgc cttatccggc
    20341 ccacaaaacc cgcaaattca attaattgcg tccccttgta ggcctgataa gcgtagcgca
    20401 tcaggcaatg cttcctgctt tataatccct tcagcaactt ttcaacaaac tcaggcacca
    20461 cctggcttgc cgggccgtaa tatttctcgg caaattcatt accgacctga cttggttcga
    20521 gattcagttc cacggtgtgc gcgccatgca gtttcgcttc gtgaacaaac ccagccgccg
    20581 gataaacatg cccggaagta ccaatggcaa tgaaaatatc agccatcgac aacgccatat
    20641 aaatttcatc catgccgagt ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg
    20701 ccgggaactg gcaacaatgg catttatctt ctggcgtaac gtctccggtc cagtcgagaa
    20761 cctgaccact ttgcgaacaa cgaactttca gcaattcccc atgcatatga atgacattgg
    20821 tattacctgc gcattcatgc aggttgtcta tattctgcgt taccagcaaa aagcgatcgc
    20881 cgagggcgtc ttgcagttta gccagcgcaa gatgcgcggc gttaggctga atttctggct
    20941 gctgcagctg tcgacgacgg gcgttataaa acgcttgcac cagttcagga tcgcgatcga
    21001 aaccttccgg cgttgccaca tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc
    21061 gaaaggtacg aatacctgat tccgcagaaa tccctgcccc tgtcagtacg agtactcttg
    21121 gtttttccat tgcttccggc accactttat ctctgaaaaa aatacgctga cgcaaacgct
    21181 cgcgcaggcg gcgtttattt ttacgaaaac gacttaaccg atgaccccga cgcgacagca
    21241 tagcaacctc tgttgtttaa tcggttagat gtaggaaggc cgcaccacgc attcctcccg
    21301 catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc tacaggtaag agatgacgag
    21361 gcagcctgtc cgccagttgc gttgtgattg ccgggaaatt cgataagcca ccaccaatga
    21421 cgaccaggtc agggtcaaca atggtcagga tatttcccag acaaaccgct aataaatcca
    21481 gataacgctc aacgtgcgcc cttgcctgct catcgccttg atcataaagc gcaataattt
    21541 cgggagcctg caacggttga tgataatagt gttgatacag ccacgcaaaa ccgcgaccag
    21601 acagataatt ttcaatgcag ccatgctgac cacagccgca gcggcgtaac gggaaatcca
    21661 accccatcat ggttaacgca tcaaccggca gacgcatatg gccaaactcg ccggtaatgt
    21721 agcttttacc ggtaatcggt ttgccgttga aaatcagccc gccgccaacg ccggtgccga
    21781 gaatcaaccc catcaccagt ggatattgag taaattcatc atcccaggct tctgaaaggg
    21841 caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc aagacgtgcg ctcaggtcgg
    21901 cacgcagcgg tttaccgctg gcagcgggga cattggcggc atacagcgta ccatcttctg
    21961 tttccggcat tcccggaata ccgatgccga cagaaccttt acagccaaaa cgttggtcag
    22021 cttcagctac cagctcacac actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg
    22081 gcacccgctt ttcccactgc aactgccgac cgctatcaaa cacgccaagc gcaatttttg
    22141 ttccaccaat atcaaacccg taatacattg ccgctccttg ttttaatgta ctgcctttac
    22201 tggccgctaa ggactcgcgc agggtcaata ttactggcgc gccgcgccgg ataccaactt
    22261 gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga agacgtccag ccagtgcaat
    22321 tccgatggca ggaaatcaat aaaatagata tcgctggaga ggaactgatg accgatcaac
    22381 ttttcaatcc actcaataat cggggtaagt tgcagtgaaa ccaccacacc gataatcacc
    22441 ccacacaggc tgccgaacag ccctgccagc aatccatacc agacaaagat ggcgcgaatt
    22501 aaaccatctt tcgcccccag cgttcttaat actgcgatat cgccactctt gtctttcacc
    22561 gccatcacta aggtggagac gatgttgaaa caggccacgc caatcaccag taccatcgcc
    22621 agatacataa tggcgcggat catctggata tcgcgataca tatagccgta agtaccaatc
    22681 caacttttaa tataaacata gctgttggtc acttcacccg catcgcgtac cagcttattg
    22741 gcgttgaaaa catccgtcat tttaagggca atacctgaca cgctggaacc catatcaaga
    22801 tattgttggg catccgccag cgggatcatg gcaaaactgt gatcgagttg accactcaac
    22861 tgcaaaatac cggcaatgtg caaacgcaca cgttttggct gcatcagttt atgctcggga
    22921 ttcgagttgg ggatcatgat cgacacccaa tcgccctgct tcactttcag cgcatccgcc
    22981 acgcctttgc cgatgataat ttgctgttcg cccgctttaa aattgcgcca ggcatccccc
    23041 tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt gcgggttaac gcccttcacc
    23101 tgaattgcgc gcagattggc accactttct accagtccgg tgaaattgat atacggcgca
    23161 gcagcggcaa tacccggcac tttttgcacg ttatccagtg cttcctgcca gttagtccat
    23221 ggctgattta ccgcctcaat ttcaccatgc ggcaccaccg ccagaatacg gttattcagt
    23281 tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca ataccgccac gccgagggca
    23341 atgccaatgg tagaaataac ggagatcagc gacaccatgc cgccgcgccg ccgtccgcga
    23401 ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca ttactccgcc cccatcaggc
    23461 tcagttccgc cgtcagacgt ccatcacgca tttccaattg gcggctcata cgtttcgcca
    23521 gttgcaggtc gtgagtaacc accaggaagg cggtgccctg caagcgattc aattccccaa
    23581 gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt accggtaggc tcatccgcaa
    23641 gtaccagacg cggattattg accagcgcac gggcaatcgc cacacgctgg cgttcgccgc
    23701 cagaaagttc agatgggcgg tgattcgcac gatgctccag ccccaccgct tttaacatct
    23761 ccagcgcacg gctgttgatt tcagcgggct ttttcttgcc aatcagcagt ggcatagcca
    23821 cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa ctgataaata aagcccagct
    23881 tctggttgcg cagttctgct ttcgccgccg aagacagttt gctcatcggt tgaccgttaa
    23941 agatcacatc gccggaggtt ggtgtatcca gcccgcccag caggtgcagc aaggtacttt
    24001 taccggaacc agagctaccg acgatcgcca tcatttcacc ttcgcccacg ctgaaactga
    24061 cattgtgtag tacatcggtt tgcacactgc cttcctgata gcgtttgcac aggttgtcgc
    24121 attgcaacag gatcttattc ataacgtaaa gcctcagcgg gttgtgtggc ggcagcgcgc
    24181 catgaagggt aaagcgtaga cagcagcgcg atagccatcg ccaccagcgc aataacaatg
    24241 acctgtaaag gttcgatagc caccggcagc gccgcgccat caagcaggac gccgattatc
    24301 ggcatcagat tatttaactg gctggcaagc agtgcgccaa gcgccgcacc gaggatcgca
    24361 ccgataatcc cggcgctggc cccttgcacc ataaagacca tcatgatttg tcgcggagtt
    24421 aagccttgcg tttgcaggat cgcgacttcg ccctgcttct ccattaccat cagccctagt
    24481 gaggtaataa tgttaaacgc cgcaacggcg acaatcaggc tcagcagcaa gcccatcata
    24541 tttttttcca tgcgtacggc ctggaacagc tcgcctttac ggtcacgcca gtcctgccat
    24601 ttgctgcctt caggcagttt ttgctgactt aaagagtcaa ctttcagcgg ctcatccagc
    24661 cacaaacgcc agccggtaat attgcctgcc ggataacgca tcagacgcga ggcatcctca
    24721 atattcacca gcatttcata gccatcgact tcactgttgg cggcgaaagt gccaatcaca
    24781 ttgaacaggc gctggcttgg aatacgcccc atcggcgtga actggctggc agatggtacc
    24841 atcacgcgga tttgatcacc gcgattaacg cctagctgtg aggcaagttg ttcgccgagg
    24901 atgacattat atttccccgg ctcgaggtca gtttgtttca cattgaccag atacggtgta
    24961 agtggatctt tttgcgccgg gtcgataccg agcatcaccc cgaccgccac gctgcgcgcg
    25021 ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg
    25081 actgccgttt ccgggagttg ctgcgggtta agagagccat gctcagaaga gagaattgcc
    25141 tgtggcatca ggccaaggat gttgttttgc agctcgcgct caaagccgtt catcactgac
    25201 aatactgtga ccagcgccat caccccgagg gtaatgccga tggtagaaag ccaggagacg
    25261 aaacgaccga agcgatccgc tgcacgccca cgcatgtaac gcaggccaat aaatagagcg
    25321 acaggttggt acatgaaatc cgtctggttg ctgtagcaaa gacacgagta tataagcgaa
    25381 agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag ggctaatctg aaaagccccc
    25441 gtaaatcaat cggctaaatt gccatgtttt atcatcaatc ttgccgcctg atcttaatct
    25501 gaaaaatcat ccaatacaga cagataccca ttacacctcg ggtcataacc cgcaggatca
    25561 accgcatgta aattaaaaaa gagcgtgttg tcctgatgaa acaaaaagag ctatggatta
    25621 accagatcaa agggttatgt atttgtctgg tggtgattta tcactcggtc attacctttt
    25681 atccgcatct gaccactttt cagcatccgt tatcggaagt cctgagcaaa tgctggatct
    25741 atttcaatct ttaccttgcc ccctttcgta tgccggtttt tttctttata tccggctatt
    25801 tgattcgccg ctatatcgac agcgtaccgt ggggaaattg tctcgataaa cggatctgga
    25861 acatcttctg ggtgctggcg ctttggggcg tggtgcagtg gctggcgtta agtgcgctga
    25921 accagtggct ggcacctgag cgcgatttaa gcaatgcctc caatgccgct tatgccgatt
    25981 ccaccggtga gttcctgcac gggatgatca ccgccagcac cagcttgtgg tatctgtatg
    26041 cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg tctggcgctg ccactattcg
    26101 ccttgtttgt actgctgagt gtggcggtta atttcgttcc cacgccgtgg tggggaatga
    26161 acagtgtgat ccgcaatttg ctttattaca gccttggcgc atggtttggc gcaacaataa
    26221 tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct gatggcttct ttgcttaccg
    26281 ttctggcggt cggtgcctgg ttgtttacta tctcgctgct gttgtcgctg gtatcgattg
    26341 tggtgatcat gaagctgttt tatcaatacg agcaacgatt cggtatgcgc tccaccagcc
    26401 tgctgaatgt gattggctcc aacaccattg ctatctacac cacccatcgc attctggttg
    26461 aaatattcag cttaactctg cttgcgcaaa tgaacgcagc acgctggtcg ccgcaagtcg
    26521 aactgacact cctgctggtt tatccctttg tcagtttgtt catctgtact gttgcgggct
    26581 tgctggtaag aaaactttca cagcgtgcat tcagcgatct gttgttctcc ccgccttcac
    26641 tgcccgctgc cgtcagttac tctcgttaaa ccatcgcccc agccggggcg attcaatttg
    26701 ctaaaccatg tcgattacgg ataatgaaca gcattgctta tcagagatat gcccccatat
    26761 gttgaggcat atcctaacga gaatctgaca accgttatgc ctgaacaata tcgttatacg
    26821 ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt taaccggcgc agcctgtgca
    26881 acgctggtag cggaaattgc cgaacgtcac gccggtccgg tggtactcat tgcaccagat
    26941 atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt tcaccgatca gatggtgatg
    27001 aatctggcgg actgggaaac tcttccctac gacagttttt cgcctcatca ggacattatc
    27061 tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc agcgtggcgt actgattgtt
    27121 ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt ttctccacgg tcatgcgctg
    27181 gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac gaacccaact ggacagcgcc
    27241 ggttatcgcc atgttgacca ggtgatggag cacggcgaat acgccacgcg cggcgcgttg
    27301 ctggatctct tcccgatggg gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa
    27361 atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca cgctggagga agtagaagcg
    27421 atcaatctgc tgcccgcgca cgaatttccg accgataaag cggcaattga actgttccgc
    27481 agccagtggc gcgatacctt cgaagtgaag cgcgatccgg aacatattta ccagcaagtg
    27541 agtaaaggca cattacctgc cgggatcgag tactggcagc cattgttctt cagcgaacca
    27601 ctgccgccgc tgttcagtta tttccctgcc aataccttgc tggtgaatac tggcgatctg
    27661 gaaaccagtg ccgaacgttt ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc
    27721 gatccgatgc gcccgctgtt gccaccacaa tcgctctggc tgcgggtgga cgagctcttc
    27781 tcagagctga aaaactggcc ccgggtgcag ctaaaaactg aacatttacc gacaaaagcc
    27841 gcgaatgcca atttaggttt ccagaaactg ccagacctgg ccgttcaggc acaacaaaaa
    27901 gcgccgctgg atgcgctgcg taagttcctc gagactttcg acggtccggt ggtgttctcg
    27961 gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct
    28021 ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg ggcgttatct gatgattggc
    28081 gctgccgaac atggttttgt cgataaggtg cgtaatctgg cgctgatctg cgaaagcgat
    28141 ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc gccgcaccat caaccccgat
    28201 acactgatcc gtaaccttgc ggagctgcat attggtcagc cggtggtcca tctggagcac
    28261 ggtgtcgggc gctatgccgg aatgaccacg ctcgaagcgg gcggcattac tggcgagtat
    28321 ttgatgctca cctatgccaa cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg
    28381 attagccgtt acgcaggtgg cgcggaagaa aacgccccgc tgcataaact tggcggcgat
    28441 gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg
    28501 ctggatatct acgcgcaacg cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag
    28561 cagtatcagt tgttctgcga cagcttcccg tttgaaacca cgccggatca ggcgcaggcc
    28621 attaatgcgg tacttagcga catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc
    28681 gatgttggct ttggtaaaac agaagtggcg atgcgcgccg ctttcctggc agtagataac
    28741 cacaagcagg tggcggtgtt ggtgcctacc acccttctcg cgcagcagca ttacgacaac
    28801 ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa tgatctcccg tttccgcagc
    28861 gccaaagagc agacgcaaat ccttgcggaa gtggcggaag ggaaaatcga tattctgatc
    28921 ggtacgcaca aactgctgca aagtgacgtc aagtttaaag atttaggcct gctgattgtc
    28981 gatgaagaac accgcttcgg ggtgcgtcat aaagagcgta ttaaagcgat gcgcgcgaac
    29041 gtggatattc tgacgcttac tgcaacgccg atcccacgta cgctgaatat ggcaatgagc
    29101 ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc
    29161 tttgtccatg agtatgacag cctggtggtc cgggaggcga tcctgcgtga aattttgcgc
    29221 ggaggacagg tttattatct ctacaatgat gtggaaaaca ttcagaaagc cgccgaacgg
    29281 ctggcagaac tggtgccaga agcgcggatc gccatcggtc acgggcagat gcgcgagcgc
    29341 gaactggaac gggtgatgaa tgatttccat catcaacgtt tcaacgtgct ggtttgtacc
    29401 accattatcg aaaccgggat cgacatcccg acagccaaca ccattatcat tgaacgtgcg
    29461 gatcacttcg gtctggcgca gctgcaccag ttacgcggtc gcgtcggacg ttcgcatcat
    29521 caggcatatg catggttgct gacgccgcat ccaaaagcga tgactaccga tgcacaaaaa
    29581 cgtcttgaag cgattgcctc gctggaagat ctcggtgcag gctttgcgct ggcaacgcac
    29641 gatctggaga ttcgcggcgc gggtgaactg cttggcgaag aacaaagcgg ctcaatggaa
    29701 accatcggtt tctcgctgta tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc
    29761 ggacgcgagc cgtcgctgga agatctcacc agccagcaaa cagaagtcga gctgcggatg
    29821 ccgtcgctat tgccagatga tttcattcct gacgtgaata cgcgtttgtc gttctataaa
    29881 cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat
    29941 cgcttcggcc tgctgccgga tccggcgcgt atcctgctgg atgttgcccg tctgcgccag
    30001 caagcgcaga aactggggat caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa
    30061 tttgccgaga agaatcacgt taatccggcc tggttgattg gtttgctgca aaaacagccg
    30121 cagcattacc gccttgatgg tccgacgcgc ctgaaattta ttcaggattt gagtgagcgg
    30181 aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac tggaagagaa cgcgatcgct
    30241 taatggtaaa tcgggggcgt ttctgcgccc ccatctgcaa catttacaaa ttctttgcac
    30301 ttccctgcac tatccgacac tgtcaccatc cataattcag gcttatctgt ttattacaat
    30361 aaccttatat ttattatgga tttttggtga tgatcaaaac gcgtttttct cgctggctaa
    30421 cgttttttac gttcgccgct gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc
    30481 cgctgccgcc agcgggcagt cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg
    30541 acggtggttc tctggaagcc atcgccaaaa aatataacgt cggctttctc gctctgttac
    30601 aggctaaccc cggcgttgat ccttacgtac cgcgcgcggg cagcgtgtta acgatcccgt
    30661 tgcaaaccct acttccagac gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc
    30721 gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc
    30781 agttaggtgg tgacacgctg acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa
    30841 acccaacctg gacgccaacg gcaaacatcc gcgcccgtta taaagcacag ggaattgagt
    30901 tgcctgcggt agtgccggct ggaccggata acccaatggg ccatcatgcg attcgtctgg
    30961 cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc cgatttcggc attggcatgc
    31021 gggtaagttc tggctgtatt cgtctgcggg atgacgatat caaaacactc tttagccagg
    31081 tcaccccagg caccaaagtg aatatcatca acactccgat aaaagtctct gccgaaccaa
    31141 acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga gaagattgat gacgatccgc
    31201 agctgctgcc aattacgctg aatagcgcaa tgcaatcatt taaagatgca gcacaaactg
    31261 acgcagaagt gatgcaacat gtgatggatg tccgttccgg gatgccggtg gatgtccgcc
    31321 gtcatcaagt gagcccacaa actctgtaaa tcctggataa aaaaaatccc cgcgacacgg
    31381 catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg agggttaatg cttatttata
    31441 aattactgct gttccatgga gggtattcgg accggttaca gaagtaatac ggaaagattt
    31501 tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc agcgatccca gatttgtccc
    31561 cgcgttagca ctgattgtac cgactttttg ttggccttct ggcgttgact gaacttcgac
    31621 agccgcaaag ctggcaaatg acatggagct taaaatcgcc gcagcgatga gggtttttac
    31681 gtttttcata atagtggcct tatgcagatg aatgacgtcg caagcaat
//
LOCUS       DAFNBJ010000044        31724 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000044 DAFNBJ010000000
VERSION     DAFNBJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.044"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(33..923)
                     /gene="rarD"
                     /locus_tag="L2X49_001888"
     CDS             complement(33..923)
                     /gene="rarD"
                     /locus_tag="L2X49_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBN4796979.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(975..1442)
                     /locus_tag="L2X49_001889"
     CDS             complement(975..1442)
                     /locus_tag="L2X49_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBN4796980.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1607..2476
                     /gene="pldA"
                     /locus_tag="L2X49_001890"
     CDS             1607..2476
                     /gene="pldA"
                     /locus_tag="L2X49_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBN4796981.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIITNMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2609..4438
                     /gene="recQ"
                     /locus_tag="L2X49_001891"
     CDS             2609..4438
                     /gene="recQ"
                     /locus_tag="L2X49_001891"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBN4796982.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4502..5122
                     /gene="rhtC"
                     /locus_tag="L2X49_001892"
     CDS             4502..5122
                     /gene="rhtC"
                     /locus_tag="L2X49_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBN4796983.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5184..5804)
                     /gene="rhtB"
                     /locus_tag="L2X49_001893"
     CDS             complement(5184..5804)
                     /gene="rhtB"
                     /locus_tag="L2X49_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBN4796984.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5915..6937
                     /gene="pldB"
                     /locus_tag="L2X49_001894"
     CDS             5915..6937
                     /gene="pldB"
                     /locus_tag="L2X49_001894"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBN4796985.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6945..7745
                     /gene="yigL"
                     /locus_tag="L2X49_001895"
     CDS             6945..7745
                     /gene="yigL"
                     /locus_tag="L2X49_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBN4796986.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTAHGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7821..8720
                     /gene="bioP"
                     /locus_tag="L2X49_001896"
     CDS             7821..8720
                     /gene="bioP"
                     /locus_tag="L2X49_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBN4796987.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8608..9561)
                     /gene="metR"
                     /locus_tag="L2X49_001897"
     CDS             complement(8608..9561)
                     /gene="metR"
                     /locus_tag="L2X49_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBN4796988.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9679..11940
                     /gene="metE"
                     /locus_tag="L2X49_001898"
     CDS             9679..11940
                     /gene="metE"
                     /locus_tag="L2X49_001898"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBN4796989.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(11979..12794)
                     /locus_tag="L2X49_001899"
     CDS             complement(11979..12794)
                     /locus_tag="L2X49_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBN4796990.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13056..13817
                     /gene="udp"
                     /locus_tag="L2X49_001900"
     CDS             13056..13817
                     /gene="udp"
                     /locus_tag="L2X49_001900"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBN4796991.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            13958..15385
                     /gene="rmuC"
                     /locus_tag="L2X49_001901"
     CDS             13958..15385
                     /gene="rmuC"
                     /locus_tag="L2X49_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBN4796992.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQYAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15480..16235
                     /gene="ubiE"
                     /locus_tag="L2X49_001902"
     CDS             15480..16235
                     /gene="ubiE"
                     /locus_tag="L2X49_001902"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBN4796993.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16249..16854
                     /gene="ubiJ"
                     /locus_tag="L2X49_001903"
     CDS             16249..16854
                     /gene="ubiJ"
                     /locus_tag="L2X49_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBN4796994.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16851..18491
                     /gene="ubiB"
                     /locus_tag="L2X49_001904"
     CDS             16851..18491
                     /gene="ubiB"
                     /locus_tag="L2X49_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBN4796995.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18570..18839
                     /gene="tatA"
                     /locus_tag="L2X49_001905"
     CDS             18570..18839
                     /gene="tatA"
                     /locus_tag="L2X49_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBN4796996.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18843..19358
                     /gene="tatB"
                     /locus_tag="L2X49_001906"
     CDS             18843..19358
                     /gene="tatB"
                     /locus_tag="L2X49_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBN4796997.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19361..20137
                     /gene="tatC"
                     /locus_tag="L2X49_001907"
     CDS             19361..20137
                     /gene="tatC"
                     /locus_tag="L2X49_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBN4796998.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20179..20961
                     /gene="tatD"
                     /locus_tag="L2X49_001908"
                     /pseudo
     CDS             20179..20961
                     /gene="tatD"
                     /locus_tag="L2X49_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
     gene            complement(20958..21446)
                     /gene="rfaH"
                     /locus_tag="L2X49_001909"
     CDS             complement(20958..21446)
                     /gene="rfaH"
                     /locus_tag="L2X49_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBN4796999.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21613..23106
                     /gene="ubiD"
                     /locus_tag="L2X49_001910"
     CDS             21613..23106
                     /gene="ubiD"
                     /locus_tag="L2X49_001910"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBN4797000.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23152..23853
                     /gene="fre"
                     /locus_tag="L2X49_001911"
     CDS             23152..23853
                     /gene="fre"
                     /locus_tag="L2X49_001911"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBN4797001.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24037..25200)
                     /gene="fadA"
                     /locus_tag="L2X49_001912"
     CDS             complement(24037..25200)
                     /gene="fadA"
                     /locus_tag="L2X49_001912"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBN4797002.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25210..27399)
                     /gene="fadB"
                     /locus_tag="L2X49_001913"
     CDS             complement(25210..27399)
                     /gene="fadB"
                     /locus_tag="L2X49_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBN4797003.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLQFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27589..28920
                     /gene="pepQ"
                     /locus_tag="L2X49_001914"
     CDS             27589..28920
                     /gene="pepQ"
                     /locus_tag="L2X49_001914"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBN4797004.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            28920..29534
                     /locus_tag="L2X49_001915"
     CDS             28920..29534
                     /locus_tag="L2X49_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBN4797005.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29573..31024
                     /gene="trkH"
                     /locus_tag="L2X49_001916"
     CDS             29573..31024
                     /gene="trkH"
                     /locus_tag="L2X49_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBN4797006.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31036..31581
                     /gene="hemG"
                     /locus_tag="L2X49_001917"
     CDS             31036..31581
                     /gene="hemG"
                     /locus_tag="L2X49_001917"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBN4797007.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7343 a         8103 c         8695 g         7583 t
ORIGIN      
        1 gtcggcatat tttttcagaa tatatttatt ttttatttgg acgttctacg ctgcgtataa
       61 atcgcatcca tcacaaaaat tgccagcgcc acccaaataa aggcgaaagt caccatctta
      121 tcggcacccg gtttttcacc ataaaacgtc acagccagca ggaacatcag cgtcgggcca
      181 atgtactgga aaaagcctaa cgttgagaga cgcaggcgcg tggcagcggc agtaaaacac
      241 aacagcggta cggtagtgac aataccggcg gcgatcagca gtaaattcag cgacatcggg
      301 ttttgcccca tatggctggt tgagctgtcg gcaatagcaa acaggtaaat cgccgccacg
      361 ggcagcagcc acatggtttc aattaacatg ccggtttgtg cttctaccgc aatcttcttg
      421 cgcaccaggc cgtagaaggc aaaactaaat gccagcccca gcgcgataat aggtagcgaa
      481 ccaaaagtcc acagctggac taacacgcca catatcgcca gaatcaccgc cagccattgc
      541 atccggcgga atcgctcgcc gaggaaaatc atccccagca caatgttcac cagcgggtta
      601 ataaagtaac caaggctcgc ttccagcata tggtgattgt tcaccgccca gataaacagt
      661 agccagttgc caccaatcag cacggcagag actgccagca taaaaatttt ctgtggcgtc
      721 tgaatcagcg tttttaaata ggaccactgg cggcaaatgc tcatcagcac caccataaag
      781 aaaaacgacc agatcacgcg atgcgtcagg atttcatcgg cgggcacgta gtaaatcaac
      841 ttgaagtacg ctggcgctat accccaaata aaataagcgg caagagcgag taatacgccc
      901 tgccgcgttt gttttgcatc catcgggaat actcattttt aattggtaac agcagtttac
      961 ctgcttttat gtcttcaacc taccatatag gtggcggtgg cactggcaat ataaagctgt
     1021 tcttcattgt gtaattcaac gcgggcgacg gcgactttat tgcctgcacg caacaggcta
     1081 ctggtagcag taaaacgctc gcccctgcct gggcgcagat aatcaacgcg aagatcaatg
     1141 gtccccatcc gcgatagccg ctggcgtagt tcatcttcac tgatggtttc gtggcgggtt
     1201 aaggtacttc ccacgcacac cagaccggcg gcgacatcca gcgccgacgc aatgactccg
     1261 ccgtgcaaaa tgctttgcgc ccagttaccc accatcatcg gctgattttt aaaggccagc
     1321 tgtgcgaact ctttttcgta acgctccagc tccatcccta acgcgcggtt aaatggcatg
     1381 tgataaacaa acatctcacc cactaatttc agggcttgtt cagcggtcag tacggcagac
     1441 atatcatcct tatacttcat tggttaatga aatgttgatt ttatgcttct ttgttgttgg
     1501 tttctacttt aggaagggat aactaacggc aatggagtta agtatgtaaa atagcccgca
     1561 gaaaaacatt caccttatca ataattcgtt acggagaaca cgaccgatgc ggactctgca
     1621 gggctggttg ttgccggtgt ttatgttgcc tatggcagta tatgcacaag aggcaacggt
     1681 gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt atcaccaata tgctgcagga
     1741 gcatgacaat ccgttcacgc tctatcctta tgacaccaac tacctcatct acacccaaac
     1801 cagcgatctg aacaaagaag cgattgccag ttacgactgg gcggaaaatg cgcgtaagga
     1861 tgaagtaaag tttcagttga gcctggcatt tccactgtgg cgtgggattt taggcccgaa
     1921 ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca atagcgaaga
     1981 gtcttcaccg tttcgtgaaa ccaactacga accgcaattg ttcctcggtt ttgccaccga
     2041 ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg gggtataacc acgactctaa
     2101 cgggcgttca gacccgacct cccgcagctg gaaccgcctt tatactcgcc tgatggcaga
     2161 aaacggtaac tggctggtag aagtgaagcc gtggtatgtg gtgggtaata ctgacgataa
     2221 cccggatatc accaaatata tgggttacta ccagcttaaa atcggctatc acctcggtga
     2281 tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc ggctacggcg gcgcggagtt
     2341 aggcttaagt tacccgatca ccaaacatgt gcgcctttat actcaggttt acagcggcta
     2401 tggcgaatcg ctcatcgact ataacttcaa ccagacccgt gtcggtgtgg gggttatgct
     2461 aaacgatttg ttttgatgaa cggttgagtg gttggcaaat ctggaatcca gcatccagga
     2521 ttaccctctc agagactaaa agcattgcag tttctcgcgc aggcgctgaa aatagcgcct
     2581 gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga atctggagtc
     2641 cggagctaaa caggttttac aagaaacctt tggctaccaa cagtttcgcc ccggccagga
     2701 agaaattatc gacactgtgc tttccggtcg cgattgcctg gtcgtcatgc ccaccggtgg
     2761 cggaaaatcc ctttgctatc aaattcctgc cttattgcta aacggcctta ccgtggttgt
     2821 ttcaccgctg atttcgttga tgaaagatca ggtggatcaa ctgcaagcca acggcgtggc
     2881 ggcggcgtgc cttaactcga cgcaaacccg cgagcagcaa cttgaagtga tgacaggctg
     2941 tcgcaccggg caaatccgcc tgctttatat cgcgccggaa cgcctgatgc tggataactt
     3001 tcttgagcat ctggcgcact ggaatccggt gttattagcc gttgatgaag cgcactgtat
     3061 ctcccaatgg ggccacgatt tccgcccgga atatgccgcg ctcggtcagt tgcgccagcg
     3121 gttcccgacg ctgccgttta tggcgctgac cgccacagcc gacgacacca cgcgccagga
     3181 tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa atcagcagtt ttgaccgtcc
     3241 gaatattcgc tacatgctga tggagaaatt caaaccgctc gatcagttga tgcgctacgt
     3301 gcaggaacag cgcggtaagt ccggcattat ctactgcaac agccgggcga aagtagaaga
     3361 caccgctgcg cgcctgcaaa gcaagggtat tagcgcggcg gcctatcatg ccgggctgga
     3421 aaataatgtc cgcgccgacg tgcaggagaa attccagcgc gatgacctgc aaattgtggt
     3481 ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg tggtccactt
     3541 tgatattccg cgcaatatcg aatcctatta tcaggaaacc ggtcgcgccg ggcgtgatgg
     3601 cctgcctgcg gaagcgatgc tgttttacga tccggctgat atggcgtggc tgcgccgttg
     3661 tctggaagag aagccgcagg ggcagttgca ggatatcgag cgccacaaac tcaatgcgat
     3721 gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta ttgctgaact attttggcga
     3781 agggcgtcag gagccgtgcg ggaactgcga tatctgcctc gatccgccga aacagtacga
     3841 cggttcaacc gatgctcaga ttgccctttc caccattggt cgtgtgaatc agcggtttgg
     3901 gatgggttat gtggtggaag tgattcgtgg tgctaataac cagcgtatcc gcgactatgg
     3961 tcatgacaaa ctgaaagtct atggcatggg ccgtgataaa agccatgaac attgggtgag
     4021 cgtgatccgc cagctgattc acctcggcct ggtgacgcaa aatattgccc agcattctgc
     4081 cctacaactg acagaggccg cgcgcccggt gctgcgcggc gaatcctctt tgcaacttgc
     4141 cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag aaatcgttcg gcggcaacta
     4201 tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg atgaaagcaa
     4261 tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt gagatggctg aacagatgcc
     4321 gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc tggaacgctt
     4381 tggtaaaccg tttatggcgc ttatccgcgc gcatgttgac ggcgacgacg aagagtagtc
     4441 agcggcataa aaaagtgcca ggatgaagac tccgtaaacg tttcccccgc gagtcaaatg
     4501 tatgttgatg ttatttctca ccgtcgccat ggtgcatatt gtggcgctta tgagccccgg
     4561 tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag aagcgatgat
     4621 gggcgtgctg ggcattacct gcggtgtaat ggtttgggct gggattgcgt tgcttggcct
     4681 gcatttgatt atcgaaaaaa tggcctggct gcatacgctg attatggttg gcggtggcct
     4741 gtatctctgc tggatgggtt accagatgct acgcggtgca ctgaaaaaag aggctgtatc
     4801 tgcgcctgca ccacagattg agctggcgaa aagcgggcgc agtttcctga aaggtttact
     4861 gaccaatctc gctaatccga aagcgattat ctactttggc tcggtgttct cattgtttgt
     4921 cggtgataac gttggcacta ccgcgcgctg gggcattttt gcgctgatca ttgtcgaaac
     4981 actggcgtgg tttaccgtcg ttgccagcct gtttgccctg ccgcaaatgc gccgtggtta
     5041 tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg ttatttgccg gatttggcat
     5101 tcatttgatt atttcgcggt gatgccagac gcgtcttcag agtaagtcgg ataaggcgtt
     5161 tacgccgcat ccgacattat ttttcacgca tgcctcgccg atgctaacag cgctcccacc
     5221 agcataaaca acgagccgaa aatcttattc agcgccttca tctgctttgg tcctttaatc
     5281 catagagcaa tccgttgagc aagggtggcg taaccgatca tcacaataat atcgactaca
     5341 atagtggtga cgccgagcac gatatactgc atcagttgcg gctgctgtgg catgatgaat
     5401 tgcggaaata gcgccgccag aaacacaata cttttgggat tggtgagatt cacaaaaact
     5461 gcgcgctgga acaaatgtcg acgcgattga gtagaggcca gcgatttaag gtcaattgca
     5521 ccagcggcgc gccactgctg gattcccagc caaatcaagt aagccgcgcc tgcccacttc
     5581 aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac gccaaccagc
     5641 acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc gccgcgataa
     5701 ccgtggttga gcgaggtggt catagtgttg attgcaccag agcctggcga caggcttaaa
     5761 atgatcgatg tcagcaggta ggcaaaccac cattctaagg tcatgatgaa ctcccggtgt
     5821 gtctattttt gtgccacaat acgctactgt cgcagcgttg tgtcaggcac gctaaaaaaa
     5881 acgattttac gtggtttaag aggcagatta cccgatgttt cagcagcaaa aagactggga
     5941 aacaagagaa aacgcgtttg ctgcttttac catgggaccg ctgactgatt tctggcgtca
     6001 gcgtgatgaa gcagagttta ctggtgtgga tgacattccg gtgcgctttg tccgttttcg
     6061 cgcacagcac catgaccggg tggtagtcat ctgcccgggg cgtattgaga gctacgtaaa
     6121 atatgcggaa ctggcctacg acctgttcca tttggggttt gatgtcttaa tcatcgacca
     6181 tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg catctcgggc atgttaatcg
     6241 ctttaatgat tatgttgatg atctggcggc attctggcag caggaggttc agcccggtcc
     6301 gtggcgtaaa cgctatatac tggcacattc gatgggcggt gcgatctcca cattatttct
     6361 gcaacgccat ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt ttgggatcgt
     6421 gattcgtatg ccgtcattta tggcacggca gatcctcaac tgggccgaag cgcatccacg
     6481 tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt ttgctatcaa
     6541 cgtactgacc cacagcagac agcgatatcg acgtaactta cgcttctatg ctgatgaccc
     6601 aacgattcgc gtcggtgggc cgacctacca ttgggtacgc gaaagtattc tggctggcga
     6661 acaggtgtta gccggtgcgg gtgatgacgc cacgccaacg cttctcttgc aggctgaaga
     6721 ggaacgcgtg gtggataacc gcatgcatga ccgtttttgt gaactccgca ccgccgcggg
     6781 ccatcctgtc gaaggaggac gaccgttggt aattaaaggt gcttaccatg agatcctttt
     6841 tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatt gttgattttt tcaacaggca
     6901 taactcaccc agcggaaacc gctctacaga ggtttaaatt tcttatgtac caggttgttg
     6961 cgtctgattt agatggcacg ttactttctc ccgaccatac gttatcccct tacgccaaag
     7021 aaaccctgaa gctgctcacc gcgcacggca tcaactttgt gtttgcgacc ggtcgtcacc
     7081 acgttgatgt ggggcaaatt cgcgataatc tggagattaa gtcttacatg attacctcca
     7141 acggcgcgcg cgttcacgat ctggatggca atctgatttt tgctcataac ctggatcgcg
     7201 acattgccag cgatctgttt ggcgtagtca acgacaatcc ggacatcatt actaacgttt
     7261 atcgcgacga cgaatggttt atgaatcgcc atcgcccgga agagatgcgc ttttttaaag
     7321 aagcggtgtt caaatatgcg ctgtatgagc ctggattact ggagccggaa ggcgtcagca
     7381 aagtgttttt cacctgcgat tcccatgaac aactgctgcc gctggagcag gcgattaacg
     7441 ctcgttgggg cgatcgcgtc aacgttagtt tctctacctt aacctgtctg gaagtgatgg
     7501 cgggcggcgt ttcaaaaggc catgcgctgg aagcggtggc gaagaaactg ggctacagcc
     7561 tgaaggattg tattgcgttt ggtgacggga tgaacgatgc cgaaatgctg tcgatggcgg
     7621 ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa agaccttcat cccgagctgg
     7681 aagtgattgg tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa ctctatttat
     7741 cgtaatcgtt ctttatttgg tcagttgtca acctgatact tcgctacaat ggatactcgt
     7801 taatcaaaga gttttccatt gtggcgctac ttatcatcac cacgattctg tgggccttct
     7861 cctttagctt ttatggcgag taccttgcgg ggcacgtcga tagctatttt gcggtgctgg
     7921 tgcgcgttgg cctggcggca ctcgtttttc tgccgtttct gcgtacccgt ggcaatagcc
     7981 tgaaaacagt cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg atgtatatgc
     8041 tgagtttccg cgcttatctc tacctgacgg tttccgaact gctgctgttc accgtactga
     8101 cgccgctcta catcacgctg atttatgaca tcatgagtaa gcgccgtctg cgctggggat
     8161 atgcttttag tgccttgttg gcggtgattg gtgccgggat tattcgctat gatcaggtca
     8221 ccgaccattt ctggactggc ttgttgctgg tgcaactctc caatatcact tttgccatcg
     8281 gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat gccgcagcat aacgcctttg
     8341 cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc atggttcttg ctgggaaatg
     8401 cgcagaaaat gccgcaaacc acgttgcaat ggggcattct ggtgtttctt ggcgtggtgg
     8461 cttccgggat tggctacttt atgtggaact acggcgcgac gcaggtggac gccggaacgc
     8521 tgggcattat gaataatatg cacgttccgg cagggctgct ggtaaacctg gctatctggc
     8581 accaacagcc gcactggcca acgttcatta caggcgcgct ggtgatcctg gcctcactgt
     8641 gggtgcatcg taagtgggtc gctccgcgct cttcacaaac ggcagatgat cgcaggcgtg
     8701 attgcgcgct gagcgaataa atgcttctgt aatcggctga cgctgctcgc catcgcgcac
     8761 ggcggcgtac agtcggctcc acaagccttc gcccagggtt ttggtcacca ccagaccctg
     8821 gcgctcaaaa ctctctacta cccaatgcgg tagcgcggca atacccatcc gcgcggcaac
     8881 catctgaatc aacaataagg tgttatcgac gcttttcagt gacgggctga cgcctgccgg
     8941 ctgaagaaaa tgccgccaga catccagtcg actacgctgc accggataaa tcaacagcgt
     9001 ctcgctggcg agatcttccg gtgtaattcg cgttttcgcc gccagtggat ggtcaggtgc
     9061 taacaccaga cgcacttcat agtcgaacat cggcgaataa tgcaggccac tgcgcggcag
     9121 gatatcggag gtcatcacca gatccagctc gccctgttgc aacgcgggct gcgggtcaaa
     9181 cgtcacgccc gatttaaaat ccatctctac ctgcggccag ttcttatgga aattttctaa
     9241 cgcgggcgtc agccactgaa tacagctatg gcactcaatg gcaatgcgca gacgcgtctg
     9301 ctgcggttca ttgcaggctt gcagtgcctg gctaatttgc ggcagcacct ggtttgccag
     9361 ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc tggctcttac gcacaaatag
     9421 ccggaagcca aggcgttgtt ccagatcgct aaactggtga gacagggcgg attgcgtctg
     9481 atgcaacgtc gccgcagcgg ctgcgagaga gccgcagttc cgcaacgctt gtagcgtttt
     9541 caggtgtttt acttcgatca tgaaagtcct tcacttcggc atgaataatt tgcgcttgag
     9601 gaatatacag taaccgccaa ttatggatgt gtaaacatct ggacggctaa atactaaaaa
     9661 ttccataagg ggcatataat gacaattctt aatcacaccc tcggtttccc tcgcgttggc
     9721 ctgcgtcgcg agctgaaaaa agcgcaagaa agttattggg cggggaactc cacgcgtgaa
     9781 gaactgctga cagtagggcg tgaactgcgt gctcgtcact gggatcaaca aaagcaagcg
     9841 ggtatcgacc tgctgccggt gggcgatttt gcctggtacg atcatgttct gaccaccagt
     9901 ctgctgctgg gtaatgttcc gccacgtcat cagaacaaag acggttcggt agatatcgac
     9961 accctgttcc gtattggtcg tggacgtgcg ccgactggcg aacctgcggc ggcagcggaa
    10021 atgaccaaat ggtttaacac caactatcac tacatggtgc cggaattcgt taaaggccaa
    10081 cagttcaaac tgacctggac gcagctgctg gaggaagtgg acgaggcgct ggcgctgggc
    10141 cacaacgtta agcctgtgct gctggggccg gttacttacc tgtggctggg gaaagtgaaa
    10201 ggtgaacagt ttgaccgcct tagcctgctg aacgacattc tgccggttta tcagcaagtg
    10261 ctggcagaac tggcgaaacg cggcatcgag tgggtacaga ttgatgaacc cgcgctggta
    10321 ctggaactac cacaggcgtg gctggacgca tacaaacccg cttacgacgc gctccaggga
    10381 caggtgaaac tgctgctgac cacctatttt gaaggcgtaa cgccaaatct cgacacgatt
    10441 actgcgctgc ctgttcaggg tctgcatgtt gaccttgtac atggtaaaga tgacgttgtt
    10501 gaactgcaca agcgcctgcc ttctgactgg ctgctgtctg cgggactgat caatggtcgt
    10561 aacgtctggc gcgccgatct tactgagaaa tatgcgcaaa ttaaggacat tgtcggcaaa
    10621 cgtgatttgt gggtggcatc ttcctgctcg ttgctgcaca gccccatcga cctgagcgtg
    10681 gaaacgcgtc ttgatgcaga agtgaaaagc tggtttgcct tcgctctaca aaaatgccat
    10741 gaactggcac tgctgcgcga tgcgctgaac agtggtgaca cggcagctct ggcagagtgg
    10801 agcgccccaa ttcaggcgcg tcgtcactct actcgtgtac ataatccggc ggtagaaaag
    10861 cgtctggcgg cgatcaccgc ccaggacagc cagcgtgcga atgtctatga agtgcgtgct
    10921 gaagcccagc gtgcgcgttt taaactgcca gcgtggccga ccaccacgat tggttccttc
    10981 ccgcaaacca cggaaattcg taccctgcgt ctggatttca aaaagggcaa tctcgacgcc
    11041 aacaactacc gcacgggcat tgcggaacat atcaagcagg ccattgttga gcaggaacgt
    11101 ttgggactgg atgtgctggt acatggcgag gccgagcgta atgacatggt ggaatacttt
    11161 ggcgagcacc tcgacggatt tgtctttacg caaaacggtt gggtacagag ctacggttcc
    11221 cgctgcgtga agccaccgat tgtcattggt gacattagcc gcccggcacc gattaccgtg
    11281 gagtgggcga agtatgcgca atcgctgacc gacaaaccgg tgaaagggat gctgacgggg
    11341 ccggtgacca tactctgctg gtcgttcccg cgtgaagatg tcagtcgtga aaccatcgcc
    11401 aaacagattg cgctggcgct gcgtgatgag gtggccgatc tggaagcggc agggattggc
    11461 atcatccaga ttgatgaacc ggcgctgcgc gaaggtttac cgctgcgtcg tagcgactgg
    11521 gacgcctatc tccagtgggg cgtagaggcc ttccgtatca acgccgccgt ggcgaaagat
    11581 gacacacaaa tccacactca catgtgttat tgcgagttca acgacatcat ggattcgatt
    11641 gcggcgctgg acgcagacgt catcaccatc gaaacctcgc gttccgacat ggagttgctg
    11701 gagtcgtttg aagagtttga ttatccaaat gaaatcggtc ctggcgtcta tgacattcac
    11761 tcgccaaacg taccgagcgt ggaatggatt gaagccttgc tgaagaaagc ggcaaaacgc
    11821 attccggcag agcgcctgtg ggtcaacccg gactgtggcc tgaaaacgcg cggctggcca
    11881 gaaacccgcg cggcactggc gaacatggtg caggcggcgc agaatttgcg tcgggggtaa
    11941 atccaaaccg ggtggtaata ccacccggtc ttttctcatt acagcgactt cttcccacca
    12001 tactgcttaa accactccag catacgctgc cagccatctt tcgcagattc ggcatgatag
    12061 ctcgggcgat aatcggcgtt gaatgcatgc ccggcatccg ggtagacgat aatctctgct
    12121 ttcgcattag cagcccgcag cgcctggcgc atggtttcaa cgctctcctg cggaatgctg
    12181 ttatcctgac caccatataa gccgagaacc ggcgcgttaa gatcggttgc gatatcaaca
    12241 ggttgtttcg gtgaattcag cgacttgtcg cccgtcagtt tgccgtacca cgccactgca
    12301 gcttttagct gtggattatg cgcggcatac agccaggtga tacgtccacc ccagcagaat
    12361 ccggtgatca ttaaacgatg aacatcgccg ccgttgcggg aagcccaact ggcgacatga
    12421 tcgagatcgg ccagcacctg cgagtcaggc acttttgcta ccagaccgct aagcaacgtg
    12481 gggatatcgg caaaatcatt cggatcgcct tcgcggaagt aaagttcagg tgcgatagcc
    12541 agatacccct ccagcgccag acggcgacaa atgtcgcgga tatgttcatg cacgccaaaa
    12601 atttcctgca ctacaatgac cactggcagt gggccatcgc tttgctttgg tctggcatgg
    12661 taagcaggca tgttatcccc ttgtgaaggg atagaggtga agcccgccac aatcgcgtcg
    12721 tccggggtct gaacgatggt cgaagcgaga ggcgatgcag caggtgcaaa tccagattgt
    12781 tgtgttgttg ccatggtatt ctccgtaccc ttataaaaat gttgcgcaat gttaactata
    12841 gtcagcatgc aacaaatcac attgcctgaa tcggctcatc ttttatgcag tcctgcagaa
    12901 tgaagagtga tttatgtgat ttgaatcact tttggtgggt aaatttatgc aacgcatttg
    12961 cgtcatggtg atgagtatca cgaaaaaatg ttaaaccctt cggtaaagtg tctttttgct
    13021 tcttctgact aaaccgattc acagaggagt tgtatatgtc caagtctgat gtttttcatc
    13081 tcggcctcac taaaaacgat ttacaagggg ctacgcttgc catcgtccct ggcgacccgg
    13141 atcgtgtgga aaagatcgcc gcgctgatgg ataagccggt taagctggca tctcaccgcg
    13201 aattcactac ctggcgtgca gagctggatg gtaaacctgt tatcgtctgc tctaccggta
    13261 tcggcggccc gtctacctct attgctgttg aagagctggc acagctgggc attcgcacct
    13321 tcctgcgtat cggtacaacg ggcgctattc agccgcatat taatgtgggt gatgtcctgg
    13381 ttaccacggc gtctgtccgt ctggatggcg cgagcctgca cttcgcaccg ctggaattcc
    13441 cggctgtcgc tgatttcgaa tgtacgactg cgctggttga agctgcgaaa tccattggcg
    13501 cgacaactca cgttggcgtg acagcttctt ctgatacctt ctacccaggt caggaacgtt
    13561 acgatactta ctctggtcgc gtagttcgtc actttaaagg ttctatggaa gagtggcagg
    13621 cgatgggcgt aatgaactat gaaatggaat ctgcaaccct gctgaccatg tgtgcaagtc
    13681 agggcctgcg tgccggtatg gtagcgggtg ttatcgttaa ccgcacccag caagagatcc
    13741 cgaatgctga gacgatgaaa caaactgaaa gccaagcggt gaaaatcgtg gtggaagcgg
    13801 cgcgtcgtct gctgtaattc tcttctcctg tctgaaggcc gacgcgttcg gccttttgta
    13861 tttttgcgta gcgcctcgca ggaaatgcct ttccaactgg acgtttgtac agcacaattc
    13921 tattttgtgc gggtaagttg ttgcgtcagg aggcgttgtg gatttctcaa tcatggttta
    13981 cgcagttatt gcgttggtgg gtgtggcaat tggctggctg tttgccagtt atcaatatgc
    14041 gcagcaaaaa gccgagcaat tagctgaacg tgaagagatg gtcgcggagt taagcgcggc
    14101 aaaacaacaa attacccaaa gcgagcactg gcgtgcagag tgcgagttac tcaataacga
    14161 agtgcgcagc ctgcaaagta ttaacacctc tctggaggcc gatctgcgtg aagtaaccac
    14221 gcggatggaa gccgcacagc aacatgctga cgataaaatt cgccagatga ttaacagcga
    14281 gcagcgcctc agtgagcagt ttgaaaacct cgccaaccgt atttttgagc acagcaatcg
    14341 ccgggttgat gagcaaaacc gtcagagtct gaacagcctg ttgtcgccgc tacgtgaaca
    14401 actggacggt ttccgccgtc aggttcagga cagcttcggt aaagaagcac aagaacgcca
    14461 taccctgacc cacgaaattc gcaatctcca gcaactcaac gcgcaaatgg cccaggaagc
    14521 gatcaacctg acgcgcgcgc tgaaaggcga caataaaacc cagggcaact ggggcgaggt
    14581 agtattgacg cgggtgctgg aggcttccgg tctgcgtgaa gggtatgaat atgaaaccca
    14641 ggtcagcatc gaaaatgacg cccgctcgcg gatgcagccg gatgtcatcg tgcggctgcc
    14701 gcagggaaaa gatgtggtga tcgacgccaa aatgacgctg gtcgcctatg aacgctattt
    14761 taacgccgaa gacgactaca cccgcgaaag cgcgctacag gaacatatcg cgtcggtgcg
    14821 taaccatatc cgtttgctgg gacgcaaaga ttatcaacag ctgccggggc tgcgaactct
    14881 ggattacgtg ctgatgttta ttcccgttga acccgctttt ttactggcgc ttgaccgcca
    14941 gccggagctg atcaccgaag cgttgaaaaa caatatcatg ctggttagcc cgactacgct
    15001 gctggtggcg ttgcgtacta ttgccaacct gtggcgttat gagcatcaaa gccgcaacgc
    15061 ccagcaaatc gccgatcgtg ccagcaagct gtacgacaag atgcgtttgt tcatcgatga
    15121 catgtccgcg attggtcaaa gtctcgacaa agcgcaggat aactaccgcc aggcaatgaa
    15181 aaaactctct tcaggtcgcg gaaatgtgct ggcgcaggca gaagcgtttc gcggtttagg
    15241 agtagaaatt aaacgcgaga ttaatccgga tttggctgaa caggcggtga gccaggatga
    15301 agagtatcga cttcggtcgg ttccggagca gccgaatgat gaagcttatc aacgcgatga
    15361 tgaatataat cagcagtcgc gctagcccat taggtgtagt taagccgggt agaaatctag
    15421 ggcatcgacg cccaatctgt tacacttctg gaacaatttt ttgatgagca ggcattgaga
    15481 tggtggataa gtcacaagaa acgacgcact ttggttttca gaccgtcgcg aaggaacaaa
    15541 aagcggatat ggtcgcccac gttttccatt ccgtggcatc aaaatacgat gtcatgaatg
    15601 atttgatgtc atttggtatt catcgtttgt ggaagcgatt cacgattgat tgcagcggcg
    15661 tacgccgtgg gcagaccgtg ctggatctgg ctggtggcac cggcgacctg acagcgaaat
    15721 tctcccgcct ggtcggagaa actggcaaag tggtccttgc tgatatcaat gaatccatgc
    15781 tcaaaatggg ccgcgagaag ctgcgtaata tcggtgtgat tggcaacgtt gagtatgttc
    15841 aggcgaacgc tgaggcgctg ccgttcccgg ataacacctt tgattgcatc accatttcgt
    15901 ttggtctgcg taacgtcacc gacaaagata aagcactgcg ttcaatgtat cgcgtgctga
    15961 aacccggcgg ccgcctgctg gtgcttgagt tctcgaagcc aattatcgag ccgctgagca
    16021 aagcctatga tgcatactcc ttccatgtgc tgccgcgtat tggctcactg gtcgcgaacg
    16081 acgccgacag ctaccgttat ctggcagaat ccatccgtat gcatcccgat caggataccc
    16141 tgaaagccat gatgcaggat gccggattcg aaagtgtcga ctactacaat ctgacggcag
    16201 gggttgtggc gctgcatcgt ggttataagt tctgacagga gaccggaaat gccttttaaa
    16261 cctttagtga cggcaggaat tgaaagtctg ctcaacacct tcctgtatcg ctcacccgcg
    16321 ctgaaaacgg cccgctcgcg tctgctgggt aaagtattgc gcgtggaggt aaaaggcttt
    16381 tcgacgtcat tgattctggt gttcagcgaa cgccaggttg atgtactggg cgaatgggca
    16441 ggcgatgctg actgcaccgt tatcgcctac gccagtgtgt tgccgaaact tcgcgatcgc
    16501 cagcagctta ccgcactgat tcgcagtggt gagctggaag tgcagggcga tattcaggtg
    16561 gtgcaaaact tcgttgcgct ggcagatctg gcagagttcg accctgcgga actgctggcc
    16621 ccttataccg gtgatatcgc cgctgaagga atcagcaaag ccatgcgcgg aggcgcaaag
    16681 ttcctgcatc acggcattaa gcgccagcaa cgttatgtgg cggaagccat tactgaagag
    16741 tggcgtatgg cacccggtcc gcttgaagtg gcctggtttg cggaagagac ggctgccgtc
    16801 gagcgtgctg ttgatgccct gaccaaacgg ctggaaaaac tggaggctaa atgacgccag
    16861 gtgaagtacg gcgcctatat ttcatcattc gcactttttt aagctacgga cttgatgaac
    16921 tgatccccaa aatgcgtatc accctgccgc tacggctatg gcgatactca ttattctgga
    16981 tgccaaatcg gcataaagac aaacttttag gtgagcgact acgactggcc ctgcaagaac
    17041 tggggccggt ttggatcaag ttcgggcaaa tgttatcaac ccgccgcgat ctttttccac
    17101 cgcatattgc cgatcagctg gcgttattgc aggacaaagt tgctccgttt gatggcaagc
    17161 tggcgaagca gcagattgaa gctgcaatgg gcggcttgcc ggtagaagcg tggtttgacg
    17221 attttgaaat caagccgctg gcttctgctt ctatcgccca ggttcatacc gcgcgattga
    17281 aatcgaatgg taaagaggtg gtgattaaag tcatccgccc ggatattttg ccggttatta
    17341 aagcggatct gaaacttatc taccgtctgg ctcgctgggt gccgcgtttg ctgccggatg
    17401 gtcgccgtct gcgcccaacc gaagtggtgc gcgagtacga aaagacattg attgatgaac
    17461 tgaatttgct gcgggaatct gccaacgcca ttcagcttcg gcgcaatttt gaagacagcc
    17521 cgatgctcta catcccggaa gtttaccctg actattgtag tgaagggatg atggtgatgg
    17581 agcgcattta cggcattccg gtgtctgacg ttgcggcgct ggagaaaaac ggcactaaca
    17641 tgaaattgct ggcggaacgc ggcgtgcagg tgttcttcac tcaggtcttt cgcgacagct
    17701 ttttccatgc cgatatgcac cctggcaaca tcttcgtaag ctatgaacac ccggaaaacc
    17761 cgaaatatat cggcattgat tgcgggattg ttggctcgct aaacaaagaa gataaacgct
    17821 atctggcaga aaactttatc gccttcttta atcgcgacta tcgcaaagtg gcagagctac
    17881 acgtcgattc aggctgggtg ccaccagata ccaacgttga agagttcgaa tttgccattc
    17941 gtacggtctg tgaacctatc tttgagaaac cgctggccga aatttcgttt ggacatgtac
    18001 tgttaaatct gtttaatacg gcgcgtcgct tcaatatgga agtgcagccg caactggtgt
    18061 tactccagaa aaccctgctc tacgtcgaag gggtaggacg ccagctttat ccgcaactcg
    18121 atttatggaa aacggcgaag cctttcctgg agtcgtggat taaagatcag gtcggtattc
    18181 ctgcgctggt gagagcattt aaagaaaaag cgccgttctg ggtcgaaaaa atgccagaac
    18241 tgcctgaact ggtttacgac agtttgcgcc agggcaagta tttacagcac agtgttgata
    18301 agattgcccg cgagcttcag tcaaatcatg tacgtcaggg acaatcgcgt tattttctcg
    18361 gaattggcgc tacgttagta ttaagtggca cattcttgtt ggtcagccga cctgaatggg
    18421 ggctgatgcc cggctggtta atggcaggtg gtctgatcgc ctggtttgtc ggttggcgca
    18481 aaacacgctg attttttcat cgctcaaggc gggccgtgta acgtataatg cggccttgtt
    18541 taatcatcat ctaccacaga ggaacatgta tgggtggtat cagtatttgg cagttattga
    18601 ttattgccgt catcgttgta ctgctttttg gcaccaaaaa gctcggctcc atcggttccg
    18661 atcttggtgc gtcgatcaaa ggctttaaaa aagcaatgag cgatgatgaa ccaaagcagg
    18721 ataaaaccag tcaggatgct gattttactg cgaaaactat cgccgataag caggcggata
    18781 cgaatcagga acaggctaaa acagaagacg cgaagcgcca cgataaagag caggtgtaat
    18841 ccgtgtttga tatcggtttt agcgaactgc tattggtgtt catcatcggc ctcgtcgttc
    18901 tggggccgca acgactgcct gtggcggtaa aaacggtagc gggctggatt cgcgcgttgc
    18961 gttcactggc gacaacggtg cagaacgaac tgacccagga gttaaaactc caggagtttc
    19021 aggacagtct gaaaaaggtt gaaaaggcga gcctcactaa cctgacaccc gaactgaaag
    19081 cgtcgatgga tgaactacgc caggccgcgg agtcgatgaa gcgttcctac gttgcaaacg
    19141 atcctgaaaa ggcgagcgat gaagcgcaca ccatccataa cccggtggtg aaagataatg
    19201 aagctgcgca tgagggcgta acgcctgccg ctgcacaaac gcaggccagt tcgccggaac
    19261 agaagccaga aaccacgcca gagccggtgg taaaacctgc tgcggacgct gaaccgaaaa
    19321 ccgctgcacc ttccccttcg tcgagtgata aaccgtaaac atgtctgtag aagatactca
    19381 accgcttatc acgcatctga ttgagctgcg taagcgtctg ctgaactgca ttatcgcggt
    19441 gatcgtgata ttcctgtgtc tggtctattt cgccaatgac atctatcacc tggtatccgc
    19501 gccattgatc aagcagttgc cgcaaggttc aacgatgatc gccaccgacg tggcctcgcc
    19561 gttctttacg ccgatcaagc tgacctttat ggtgtcgctg attctgtcag cgccggtgat
    19621 tctctatcag gtgtgggcat ttatcgcccc agcgctgtat aagcatgaac gtcgcctggt
    19681 ggtgccgctg ctggtttcca gctctctgct gttttatatc ggcatggcat tcgcctactt
    19741 tgtggtcttt ccgctggcat ttggcttcct tgccaatacc gcgccggaag gggtgcaggt
    19801 atccaccgac atcgccagct atttaagctt cgttatggcg ctgtttatgg cgtttggtgt
    19861 ctcctttgaa gtgccggtag caattgtgct gctgtgctgg atggggatta cctcgccaga
    19921 agacttacgc aaaaaacgcc cgtatgtgct ggttggtgca ttcgttgtcg ggatgttgct
    19981 gacgccgccg gatgtcttct cgcaaacgct gttggcgatc ccgatgtact gtctgtttga
    20041 aatcggtgtc ttcttctctc gcttttacgt tggtaaaggg cgaaatcggg aagaggaaaa
    20101 cgacgctgaa gcagaaagcg aaaaaactga agaataaatt caaccgcccg tcagggcggt
    20161 tgtcatatgg agtacaggat gtttgatatc ggcgttaatt tgaccagttc gcaatttgcg
    20221 aaagaccgtg atgatgttgt agcgcgcgct tttgacgcgg gagttaatgg gctactcatc
    20281 accggcacta acctgcgtga aagccagcag gcgcaaaagc tggcgcgtca gtattcgtcc
    20341 tgttggtcaa cggcgggcgt acatcctcac gacagcagcc agtggcaagc tgcgactgaa
    20401 gaagcgatta ttgagctggc cgcgcagcca gaagtggtgg cgattggtga atgtggtctc
    20461 gactttaacc gcaacttttc gacgccggaa gagcaggaac gcgcttttgt tgcccagcta
    20521 cgcattgccg cagatttaaa catgccggta tttatgcact gtcgcgatgc ccacgagcgg
    20581 tttatgacat tgctggagcc gtggctggat aaactgcctg gtgcggttct tcattgcttt
    20641 accggcacac gcgaagagat gcaggcgtga gtggcgcatg gaatttatat cggcattacc
    20701 ggttgggttt gcgatgaacg acgcggactg gagctgcggg aacttttgcc gttgattccg
    20761 gcggaaaaat tactgatcga aactgatgcg ccgtatctgc tccctcgcga tctcacgcca
    20821 aagccatcat cccggcgcaa cgagccagcc catctgcccc atattttgca acgtattgcg
    20881 cactggcgtg gagaagatgc cgcatggctg gctgccacca cggatgctaa tgtcaaaaca
    20941 ctgtttggga ttgcgtttta gagtttgcgg aactcggtgt tcttcacact gtgcttaatc
    21001 tctttattaa taagattaag caatagcatg gagcgagcct caccatcggg ttcggtgaaa
    21061 atggcctgaa agccttcgaa cgcgccttcg gtaataatca ccttatcacc cggataaggg
    21121 gttgccggat cgacaatgtc tttcggttta tataccgata gctgatgaat caccgccgat
    21181 gggactatcg ctggcgacgc gccaaagcgc acgaagtggc tgacaccgcg ggtcgcgttg
    21241 atagtcgtgg tatgaatcac ttctggatca aattcgacaa acaggtagtt gggaaacaat
    21301 ggctcactga ctgcagtacg ttttccacgc acgatttttt ccagggtgat catcggtgcc
    21361 aggcaattca cagcctgtct ttcgaggtgt tcctgggcac gttgaagttg cccgcgcttg
    21421 cagtacagta aataccagga ttgcataatg actcttatcc gtttaatcgg ggcgcaagga
    21481 tagcaaaagc tttacgctaa gttaattata ttccccggtt tgcgttatac cgtcagagtt
    21541 cacgctaatt taacaaattt acagcatcgc aaagatgaac gccgtataat gggcgtagat
    21601 taagaggcta caatggacgc catgaaatat aacgatttac gcgacttcct gacgctgctt
    21661 gaacagcagg gtgagctaaa acgtatcacg ctcccggtgg atccgcatct ggaaatcact
    21721 gaaattgctg accgcacttt gcgtgccggt gggcctgcgc tgttgttcga aaaccctaaa
    21781 ggctactcaa tgccggtgct gtgcaacctg ttcggtacgc caaagcgcgt ggcgatgggc
    21841 atggggcagg aagatgtttc ggcgctgcgt gaagttggta aattattggc gtttctgaaa
    21901 gagccggagc cgccaaaagg tttccgcgac ctgtttgata aactgccgca gtttaagcaa
    21961 gtattgaaca tgccgacaaa gcgactgcgt ggtgcgccct gccaacaaaa aatcgtctct
    22021 ggcgatgacg tcgatctcaa tcgcattccc attatgacct gctggccgga agatgccgcg
    22081 ccgctgatta cctgggggct gaccgtaacg cgcggcccgc ataaagagcg gcagaatctg
    22141 ggcatttatc gtcagcagct gattggtaaa aacaaactga ttatgcgctg gctgtcgcat
    22201 cgcggcggcg cgctggatta tcaggagtgg tgtgcggcgc atccgggcga acgtttcccg
    22261 gtttctgtgg cgctgggtgc cgatcccgcc acgattctcg gtgcagtcac ccccgttccg
    22321 gatacgcttt cagagtatgc gtttgccgga ttgctacgtg gcaccaagac cgaagtggtg
    22381 aagtgtatct ccaatgacct tgaagtgccc gccagtgcgg agattgtgct ggaagggtat
    22441 atagaacaag gcgaaactgc tccggaaggg ccgtatggcg accacaccgg ttactataac
    22501 gaagtcgata gtttcccggt atttaccgtg acgcatatta cccagcgtga agatgcgatt
    22561 taccactcca cctatactgg tcgtccgccg gatgaacccg cggtgctggg tgtcgcactg
    22621 aacgaagtgt ttgtgccgat tctgcaaaaa cagttcccgg aaattgtcga tttttacctg
    22681 ccgccggaag gctgttctta tcgtctggcg gtagtgacga taaaaaaaca gtacgccgga
    22741 cacgcgaagc gcgtcatgat gggcgtctgg tcgttcttac gccagtttat gtacactaag
    22801 tttgtgatcg tttgcgatga tgacgtcaac gcacgcgact ggaacgatgt gatttgggcg
    22861 attaccaccc gtatggatcc ggcgcgggat actgttctgg tagaaaatac gcctattgat
    22921 tatctggatt ttgcctcgcc tgtttccggg ctgggttcaa aaatggggct ggatgccacg
    22981 aataaatggc cgggggaaac ccagcgtgaa tggggacgtc ccatcaaaaa agatccagat
    23041 gttgtcgcgc atattgacgc catctgggat gaactggcta tttttaacaa cggtaaaagc
    23101 gcctgatgtg cgtttgtttt gccctattta tcgatccgac agagaaagcg catgacaacc
    23161 ttaagctgta aagtgacctc ggtagaagct atcacggata ccgtatatcg tgtccgcatc
    23221 gtgccagacg cggccttttc ttttcgtgct ggtcagtatt tgatggtagt gatggatgag
    23281 cgcgacaaac gtccgttctc aatggcctcg acgccggatg aaaaagggtt tatcgagctg
    23341 catattggcg cttctgaaat caacctttac gcgaaagcag tcatggaccg catcctcaaa
    23401 gatcatcaaa tcgtggtcga tattccccac ggagaagcgt ggctgcgcga tgatgaagag
    23461 cgtccgatga ttttgattgc gggcggcacc gggttctctt atgcccgctc gattttgctg
    23521 acagcgttgg cgcgtaaccc aaaccgtgat atcaccattt actggggcgg gcgtgaagag
    23581 cagcatctgt atgatctctg cgagcttgaa gcgctttcgt taaagcaccc tggtctgcaa
    23641 gtggtgccgg tggttgaaca accggaagcg ggctggcgtg ggcgtactgg caccgtgtta
    23701 acggcggtat tgcaggatca cggtacgctg gcagagcatg atatctatat tgccggacgt
    23761 tttgagatgg cgaaaatagc ccgtgacctg ttttgtagtg agcgtaatgc gcgggaagat
    23821 cgcctgtttg gcgatgcgtt tgcatttatc tgagatataa aaaaacccgc ccctgacagg
    23881 cgggaagaac ggcaactaaa actttattca gtggcattta gatctatgac gtatctggca
    23941 aaccatgccc gatgcgacgc tgtcgcgtct tatcgtgcct acaaatagtc cgaaccgtag
    24001 gccggataag gcgtttacgc cgcatccggc aagtggttaa acccgctcaa acaccgtcgc
    24061 aataccctga cccagaccga tacacatcgt cgccagacca aactgaacgt ctttgcgttc
    24121 catcagattc agcagcgtgg tgctgatacg cgcaccggaa cagcccagcg gatgacccag
    24181 cgcgatcgca ccaccattga ggttgatctt ctcgtcaatc tgctccatta gcccgagatc
    24241 tttaatgcac ggcaggatct gcgcggcaaa ggcttcgttc atctcaaaca cgccgatatc
    24301 gctggcagaa agccccgctt ttttcagcgc cagcttcgag gccggaaccg ggccgtaacc
    24361 cataatcgat gggtcacaac caacgaccgc catcgaacgc acacgagcgc gcggcttaag
    24421 acctaattca tgggcgcggc tttcactcat caccagcatg gcagctgcgc catcggaaag
    24481 tgcagaagat gtgcccgccg ttaccgtacc gtttactgga tcaaacgccg gacgcagcgt
    24541 ggcgagggct tccacggtgg tttccgggcg aatcacttcg tcgtaattaa actgcttcag
    24601 gacgccgtcg gcatcgtgac caccagttgg gatgatttca tttttaaatg cggccgactg
    24661 cgtggcggcc caggcgcggg cgtgtgaccg cgcggcaaag gcatcctgca tttcacggct
    24721 gataccgtgc atacgcgcca gcatttctgc tgttaagccc atcatgcccg ccgctttggc
    24781 gacattgcgg ctcaggccgg ggtgaaaatc gacgccgtga ctcatcggca catggcccat
    24841 atgctccacg ccgccaacca gacatgcctg cgcatcgccg gtcatgatca ttcgtgctgc
    24901 gtcatgcagt gcctgcatgg atgaaccaca caagcgattg acggtaaccg ccgggacaga
    24961 gtgcggcact tctgccagca gcgccgcgtt acgggcgatg ttaaaaccct gctccagcgt
    25021 ctgctgcaca caaccccagt aaatatcgtc gagggccgcc gcttccagcg ccgggttacg
    25081 cgccagcagg ctacgcatta aatgagcgga gagatcttcc gcacgcacgt tacgaaaagc
    25141 accgcccttc gaacggccca tcggggtgcg aattgcatcg acaatgacaa cctgttccat
    25201 tgtgactcct taagccgttt tcaggtcgcc aaccggacgg gctggctcaa ccgggggata
    25261 gtacggttcg ttatgacgtg ctttattacg cagaccttcc ggcacttcgt acagcgggcc
    25321 gaggtgctga tattgctgtg ccatatcgag gtattttgcg ctaccgaggg tgtccagcca
    25381 gcggaacgcg ccgccgtgga acggagggaa gcccaggccg tagaccagcg ccatatccgc
    25441 ttccgccgga gtggcgataa tgccttcctc cagacagcgc accacttcgt tgaccatcgg
    25501 gatcatcatg cgggcgataa tctcttcttc gctgaaatcg cgcttcggct ggctcacttc
    25561 tgccagcagg tcttcaacgg cggcgtcttc ttctttcttc ggcttacctt tgctgtcttc
    25621 tttataacgc cagaaaccga ggccgttctt ctgaccaaag cggttggcat caaacagcgc
    25681 gtcgatggca tcgcggtaat ctttctgcat ccgctgcggg aagcctgctg ccatgacagc
    25741 ctgagcgtga tgcgcggtat caatgcccac gacatccagc agatatgccg gacccatagg
    25801 ccagccaaac tgtttttcca tcactttgtc gatcttgcgg aaatccgcgc cgtcgcgcag
    25861 cagctggctg aaaccggcga aatacgggaa cagcacgcgg ttaacaaaga agccggggca
    25921 gtcgttaacc acaatcggcg tcttgcccat cttgctcgcc caggcgacaa ctttcgcgat
    25981 ggtttcgtcg gagcttttct cgccgcgaat aatttctacc aacggcatcc ggtgtaccgg
    26041 gttaaaaaag tgcattccgc agaagttttc cgggcgttcc agcgcgttgg ccagttcgct
    26101 gataggaatg gttgaagtgt tagacgccag cacggtatcc gggcgtactt tttgttcggt
    26161 ttctgccagt acggcttttt tcactttcgg gttttcgaca accgcttcta ccacaacatc
    26221 cacgcggtca aacccggcgt agtcgagcgt tgggtggatt gtggagatca cgccagccag
    26281 tttcagacca tcgatcttgc cgcgctcaag ctgcttgttc agcagtttcg cggcttcggt
    26341 catgccgagg gttaacgact tgtcgttgat atctttcatg acaaccggca cgcctttcca
    26401 cgcagactgg taagcgatgc cgccgcccat aatgcctgca cccagcaccg cggcctgttt
    26461 cggggtttca acgtctttgg tgagtttctt cgctttgcct tttacatatt gatcgttaag
    26521 gaaaatgccg accagtgcgc gggcttcgtt ggtatgcgcc agcgggacaa aacttttgtt
    26581 ttccaggttt aaggcttctt cacgaccaaa acgggccgca gcttcaatgg tttttactgc
    26641 ggtgatgggg gccggataat gtttccccgc tgtttgtgcg accatccctt tagcgatggt
    26701 gaagctcatg gtggcttcaa tcttgctcag ttttagtggt tccagcttcg gctgacgttt
    26761 tgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa accgcctttg cgccttcaac
    26821 cagtttttct gctttgacta cgccatccac cagaccgatt ttcagcgcct gatccgcgcc
    26881 gacatcttta ccggcggcaa tgatttccag cgcactgtca gcgcccagca tacgtggcat
    26941 acgtacagaa ccgccaaagc ccggcatgat acccagtttg gtttccggca gaccgatgcg
    27001 cagatccggc gtcgccagac gataatcggt cgccagcacg cattcgcagc caccgcccag
    27061 cgcatagccg ttgacggcag caatggtcgg caccggcaga tcttccaggc gattaaacac
    27121 gctattggca aattgcagcc actgacttaa ctgttcttca ggaacgagga acagggacaa
    27181 aaattcggtg atatcagcac cgacgataaa ggctgctttg ttcgaacgca gcagcagccc
    27241 ttttagatct gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg cgacggtcgc
    27301 agtgtcgagt ttattaactg aacctggggc atcaaatacc agttcggcaa tgccatcttc
    27361 cagccagtca aggtacaggg tgtcgccttt gtaaagcatg tcagtctcct gaatccgcaa
    27421 ggtgatctgg tcgtaccaga tgagtcgaag tgtgtatttt gtgttaaaaa tatgcaaata
    27481 aaagattaaa gaaatgccga tctgatcacg ctcggcagaa atcacgctct ggatgaacga
    27541 tgtgctaaga tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat ggaatcactg
    27601 gcctcgctct ataaaaatca tatagctacc ttacaagaac ggactcgcga tgcgctggcg
    27661 cgcttcaagc tggatgcgtt acttattcac tccggcgagc tgttcaatgt ttttctcgac
    27721 gatcatccct atccgtttaa agtgaacccg caattcaaag cgtgggtgcc ggtaactcag
    27781 gtgccaaact gctggttgct ggtggatggc gtgaacaagc cgaaactgtg gttttatctg
    27841 ccggttgatt actggcacaa cgtcgaaccg ctgccgacct ccttctggac tgaagatgtg
    27901 gaagtgatcg cgctgccgaa agccgatggc attggtagcc tgctgcctgc tgcgcgcggc
    27961 aatatcggtt atatcggtcc ggtgccggag cgtgcgctgc aactgggtat tgaggccagc
    28021 aacatcaacc cgaaaggggt tatcgactac ctgcattact atcgctcctt caaaaccgag
    28081 tacgaactgg cctgtatgcg tgaagcgcag aaaatggcgg tcaacggtca tcgtgcggca
    28141 gaagaagcgt tccgttctgg catgagcgag ttcgatatca acattgccta tctgaccgcg
    28201 accggtcatc gtgataccga cgtaccttac agcaacattg tggcgcttaa cgaacacgct
    28261 gcggtgctgc attacaccaa actggatcat caggcgccgg aagagatgcg cagcttcctg
    28321 ctggatgccg gggccgaata taacggctat gcggctgacc tgactcgtac ctggtcggca
    28381 aaaagcgaca acgactacgc acagctggtg aaagacgtaa atgatgaaca actggcgctg
    28441 atcgccacca tgaaagcagg cgttagctat gtggattacc acatccagtt ccatcagcgc
    28501 atcgccaaac tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga agcgatggtc
    28561 gaaaacgatc tcaccggacc gtttatgccg cacggtatcg gtcatccgct gggcctgcag
    28621 gtgcatgacg tagccggttt tatgcaggat gatagcggta cacacctcgc ggcaccggca
    28681 aaatatccgt acctgcgctg cacccgtatt ctccagccgg gcatggtgtt aaccatcgaa
    28741 ccgggtatct acttcattga atcgctactg gcaccgtggc gtgaagggca gttcagcaag
    28801 cacttcaact ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg tatcgaagac
    28861 aacgtggtga tccacgaaaa caacgtggaa aacatgaccc gggatctgaa actggcgtga
    28921 tggaaagctg gttaattcct gcggcaccgg tcacggtcgt tgaagagatc aaaaagagcc
    28981 gcttcataac actgttggcg cataccgatg gcgttgaggc ggcgaaagcg tttgttgaat
    29041 cggtgcgggc agaacacccc gatgcccgcc accattgcgt ggcgtgggtg gcgggtgcgc
    29101 cggatgattc tcaacagctg ggtttctctg acgacgggga gccggcagga acggcaggta
    29161 aaccgatgct cgcccagcta atgggcagcg gcgtcgggga aattaccgct gtggtagtgc
    29221 gctactacgg tggcatattg cttggcaccg gtgggttagt gaaagcgtat ggcggcggcg
    29281 tgaatcaggc gctgcgccag ctaacgaccc aacgcaagac gccattaacc gaatatactt
    29341 tgcaatgtga atatagtcag ttaaccggca ttgaagcgtt gctggggcag tgtgacggca
    29401 aaattatcaa cagtgattat caggcattcg ttctgctgcg ggtggcgctt ccggcggcga
    29461 aagtggctga attttccgca aagctggcgg attttagccg tggttcattg caattgttag
    29521 cgattgaaga ataatcccca cttcgttttg cagaactaag gaagcggcag agatgcattt
    29581 tcgcgccatt acccgaatcg ttggactact ggtcatctta ttttcaggga ccatgattat
    29641 ccctgggctg gtagcactca tctaccggga tggagcgggc cgcgctttta cccagacctt
    29701 ttttgtcgcc ctcgccattg gttctatgct gtggtggccg aaccgtaaag agaagggcga
    29761 actgaaatcc cgtgaggggt ttctgatagt ggtgctgttc tggaccgtgc tgggtagtgt
    29821 cggtgcgctc ccttttatct tctcggaaag cccgaacctc acgattaccg atgcgttttt
    29881 tgaatctttc tctggcctga ccaccaccgg ggccactacg ctggtggggc tggattcgct
    29941 ccctcacgcc atcctctttt atcgccagat gctgcaatgg tttggcggga tggggatcat
    30001 cgtgttagcg gttgcgatac tgcctatcct cggcgtgggt gggatgcagc tctatcgcgc
    30061 agaaatgccc ggcccgctga aagataacaa aatgcgcccg cgaattgcgg aaacggcgaa
    30121 aaccctgtgg ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt tgtggtttgc
    30181 cggaatggat gcctttgatg ccatcggcca tagctttgcg actatcgcta ttggcggctt
    30241 ctcgacacat gatgccagta tcggttattt cgacagcccg actattaaca ctatcattgc
    30301 tatcttcctg ctgatctccg gctgtaacta cggtctgcac ttttcactgt taagtgggcg
    30361 tagtctgaag gtttattggc gcgatccgga atttcgcatg tttatcggcg tacagtttac
    30421 gctggtggtt atttgtaccc tcgtactgtg gtttcacaat gtctacagtt cggcgctgat
    30481 gacaattaac caggcgtttt tccaggtggt gtcgatggcg acaaccgccg ggtttacgac
    30541 tgacagcatt gcccgctggc cgctcttttt gccggtactg cttttatgtt cagcttttat
    30601 cggcggttgt gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc tgctgctgtt
    30661 taagcagggg aaccgtgagc tgaaacgact ggtgcatccg aacgccgtct atagcattaa
    30721 gctggggaat cgcgcactgc cggaacgtat cctcgaagcc gtttggggat ttttctccgc
    30781 ctatgcattg gtgtttattg tcagtatgct ggcgattatc gccacgggcg tggatgactt
    30841 ttctgctttt gcctcggttg ttgcgacatt gaataacctg gggccggggc ttggcgtggt
    30901 tgctgataac tttaccagta tgaacccggt ggctaaatgg atcctgattg ccaacatgct
    30961 gtttggtcgt ctcgaggtct ttacattgct ggtgctcttt accccgactt tctggcgtga
    31021 atgatggagt aatacgtgaa aacattaatt cttttctcaa caagggacgg acaaacgcgc
    31081 gagattgcct cctacttggc ttcggaactg aaagaactgg ggatccaggc ggatgtcgcc
    31141 aatgtgcacc gcattgaaga accacagtgg gaaaactacg accgtgtggt cattggtgct
    31201 tctattcgct atggtcacta ccactctgcg ttccaggaat ttgtcaaaaa gcatgcgacg
    31261 cggctgaatt cgatgccgag cgccttttac tccgtaaacc tggtggcgcg taaaccggag
    31321 aagcgcactc cacagaccaa cagctacgcg cggaagtttc tgatgaactc gcaatggcgt
    31381 cccgatcgct gcgcggtcat tgccggggcg ctgcgttacc cacgttatcg ctggtacgac
    31441 cgttttatga tcaagctaat tatgaagatg tcaggcggtg aaacggatac gcgcaaagaa
    31501 gttgtctata ccgattggga gcaggtggcg aatttcgccc gagaaatcgc ccatttaacc
    31561 gacaaaccga cgctgaaata agcataaaga ataaaaaatg cgcggtcgga aaattatttt
    31621 aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca
    31681 acggcaaaca cgccgccggg tcagcggggt tctcctgaga attc
//
LOCUS       DAFNBJ010000045        31504 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000045 DAFNBJ010000000
VERSION     DAFNBJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.045"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(118..1491)
                     /gene="argH"
                     /locus_tag="L2X49_001918"
     CDS             complement(118..1491)
                     /gene="argH"
                     /locus_tag="L2X49_001918"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBN4797008.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1552..2328)
                     /gene="argB"
                     /locus_tag="L2X49_001919"
     CDS             complement(1552..2328)
                     /gene="argB"
                     /locus_tag="L2X49_001919"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBN4797009.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(2336..3340)
                     /gene="argC"
                     /locus_tag="L2X49_001920"
     CDS             complement(2336..3340)
                     /gene="argC"
                     /locus_tag="L2X49_001920"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBN4797010.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3494..4645
                     /gene="argE"
                     /locus_tag="L2X49_001921"
     CDS             3494..4645
                     /gene="argE"
                     /locus_tag="L2X49_001921"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBN4797011.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            4997..7648
                     /gene="ppc"
                     /locus_tag="L2X49_001922"
     CDS             4997..7648
                     /gene="ppc"
                     /locus_tag="L2X49_001922"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBN4797012.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7831..9564
                     /locus_tag="L2X49_001923"
     CDS             7831..9564
                     /locus_tag="L2X49_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBN4797013.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9713..10564
                     /locus_tag="L2X49_001924"
     CDS             9713..10564
                     /locus_tag="L2X49_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN4797014.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10551..10892)
                     /locus_tag="L2X49_001925"
     CDS             complement(10551..10892)
                     /locus_tag="L2X49_001925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBN4797015.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10894..11772)
                     /locus_tag="L2X49_001926"
     CDS             complement(10894..11772)
                     /locus_tag="L2X49_001926"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBN4797016.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11738..14035)
                     /locus_tag="L2X49_001927"
     CDS             complement(11738..14035)
                     /locus_tag="L2X49_001927"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBN4797017.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGTRYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14086..14406)
                     /locus_tag="L2X49_001928"
     CDS             complement(14086..14406)
                     /locus_tag="L2X49_001928"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBN4797018.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14421..15500)
                     /locus_tag="L2X49_001929"
     CDS             complement(14421..15500)
                     /locus_tag="L2X49_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBN4797019.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLISRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15809..18310
                     /gene="ptsP"
                     /locus_tag="L2X49_001930"
     CDS             15809..18310
                     /gene="ptsP"
                     /locus_tag="L2X49_001930"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBN4797020.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18322..18984
                     /gene="fsa"
                     /locus_tag="L2X49_001931"
     CDS             18322..18984
                     /gene="fsa"
                     /locus_tag="L2X49_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBN4797021.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            18995..20098
                     /gene="gldA"
                     /locus_tag="L2X49_001932"
     CDS             18995..20098
                     /gene="gldA"
                     /locus_tag="L2X49_001932"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HBN4797022.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            20373..20990
                     /locus_tag="L2X49_001933"
     CDS             20373..20990
                     /locus_tag="L2X49_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBN4797023.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21017..21922)
                     /gene="yijE"
                     /locus_tag="L2X49_001934"
     CDS             complement(21017..21922)
                     /gene="yijE"
                     /locus_tag="L2X49_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBN4797024.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRAMRPTPFKYTLAIALLQTCGMIGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALVYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGVVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22016..24196)
                     /gene="katG"
                     /locus_tag="L2X49_001935"
     CDS             complement(22016..24196)
                     /gene="katG"
                     /locus_tag="L2X49_001935"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBN4797025.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24525..25415)
                     /gene="metF"
                     /locus_tag="L2X49_001936"
     CDS             complement(24525..25415)
                     /gene="metF"
                     /locus_tag="L2X49_001936"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBN4797026.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25764..28196)
                     /gene="metL"
                     /locus_tag="L2X49_001937"
     CDS             complement(25764..28196)
                     /gene="metL"
                     /locus_tag="L2X49_001937"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBN4797027.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28199..29359)
                     /gene="metB"
                     /locus_tag="L2X49_001938"
     CDS             complement(28199..29359)
                     /gene="metB"
                     /locus_tag="L2X49_001938"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBN4797028.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29636..29953
                     /gene="metJ"
                     /locus_tag="L2X49_001939"
     CDS             29636..29953
                     /gene="metJ"
                     /locus_tag="L2X49_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBN4797029.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30137..30745
                     /locus_tag="L2X49_001940"
     CDS             30137..30745
                     /locus_tag="L2X49_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBN4797030.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            30890..31504
                     /locus_tag="L2X49_001941"
                     /pseudo
     CDS             30890..31504
                     /locus_tag="L2X49_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         7595 a         8793 c         8003 g         7113 t
ORIGIN      
        1 caccagaatg ctgacaaatt tctgcctgat gtggcgtaaa cgccttatcc gcctacgaag
       61 tgtcgcactt tgtaggcgtg gtaagctccc cattttcagg ggagcaatat aaacaaatta
      121 ctctaaccgc gcctgcgcaa aagcaattgc ctgcgccacc tgctgcggtg agacgccgcc
      181 ttttgccgca cgcttgtcga ggcacgattg cagcgacaga atcggataga catcttcgtc
      241 aatcaccggg ctgaatttct gcaactcgtc gagcggcaga tcttccagcg gtttgccctg
      301 acgaatggct tccaccaccg cttcaccaac aatatggtgc gcctcgcgga acggtacgcc
      361 tttcgccacc agataatccg ccagttcggt ggcgttggcg taaccctgct gagccgcttc
      421 ctggcaacgt ggacgtttca cctgaatgcc gtccagcacc agcgccgcca tatgcaggca
      481 gtccagccag gtatcgagcg cgtcgaacag accttctttg tcttcctgca tatctttgtt
      541 gtaagccagc ggcaaacctt tcagcgtcat catcatgccg gttaacgccc cctgcacccg
      601 accgcattta ccgcgaatca gctccagcgc atccgggttt ttcttctgcg gcattaatga
      661 tgaaccggaa gtcacgcggt cagaaagctc cacaaacccc gcttcgccgg tgttaaagaa
      721 aatcagatct tcagcaaaac gcgacagatg caccatgccg atagcggcag cagaaagcag
      781 ttccaacaca tggtcacggt cagaaacgct gtcgagactg ttacgggtcg ccgaagcaaa
      841 gcccagccag cctgctaact gttcacggtc gatttcatag gccgttcccg ccagcgcgcc
      901 acagcctagc gggctgacat ccagacgctt aagcgcatcc tgcaaacggc tttcatcacg
      961 cgccagcatc tcaacatagg ccaggcacca gtgcgcgaac gtcaccggct gggcgcgttg
     1021 caggtgagtg taacctggca ttaccgcgtc ctgattgttt tgtgcggttt ccaccagcgc
     1081 cgattgcagc tgccggttag ccgtcagtaa ctcgctaacg gtatctttgc accacagttt
     1141 caggtcagtc gctacctgat cattacggct acgcccggta tgcagctttt tgcctaactg
     1201 gcccactttg tcgatcagtt tgccttccac ccagctatgg atatcttcgg cgtcgctttc
     1261 aaggatttgt tgtggcctgg cgcgaacatc ttccagcaac acgttcagcg cctcttccag
     1321 ttgcgcctgc tcttctgcgg ttaacacgcc taccgtgacc agggctttgg accaggccac
     1381 agagccaaca atatcctgct ccgccagacg gtaatcaaag cgcagtgagt cgttgaattg
     1441 tttgaaccgt tgatctgctg cctgggtaaa acgcccgccc caaagtgcca taactctgtt
     1501 tccttatttt gaaattcaat gccggaaagc tgcgcctccg gccaacaaaa cttaagctaa
     1561 aatccgcgta cccatcggca taccgttaaa cagtgccgga agctgctccg catgacgcca
     1621 ggaggcgata tctaccggac ggcccagcgt gcgggccgca tccagcgccg cgttcacttt
     1681 cactatcatg ccgtcagtaa taatgccctg ctcaatcagt tgttctgctt tcgcggcggt
     1741 catttcggca atgcgttgcc ctttgccgtc gagaatgccg ctgacgtcgg agagcaaaat
     1801 cagatccgcg cccagcgttg ccgccagcgc cgttgccgcc tggtcggcat tgacgttcat
     1861 cagttgccct tcgtctgtta cgccaatgga gctgaccacc ggcagataac cgttctccag
     1921 caaggagttg ataagcttag gcgaacctgg ctgcgccagt ccaacatgac ctaactcttc
     1981 atcaagctgg gtcactttga cgctgtcgcc gtcaccgaga aacaaaccta cggccgcaat
     2041 ctgatgtttc ttcgcccatg ccaacagggt tttatttgcc gttcccgcca gtgctccggt
     2101 gataatgtct atctgatcag caggcgtcac ccgcaggccg tttttctttt tcaccggcag
     2161 attcagccct ttcatcagct catccaccac gcaaccgccg ccgtgcacaa tcaccagcgg
     2221 acgctgatgt gactcacgat aattcaccag tgcgctaaac agacgttcca gcgcctcttc
     2281 actatccagc agtacgccgc ccagtttgat aattaatgga ttcatcattg cacccttaaa
     2341 taagagactg cgtttccgca tagccgaaac gaatattggc gcactgtacc gcttgtgccg
     2401 ccgcgccttt cagtaagttg tcttcggtcg ccacaataat cagatgctcg ccctgaacgg
     2461 caaacccgat atcgcaaaat ggcagcccaa cgacattttt cagcgccgga acgcctttgt
     2521 catacagccg caccagcggt ttatgggcat acgcctgctg taacacttgc gcgacttgcg
     2581 cctgggtcac acccgatttc aggcggcagg taatggtttc gagaatgccg cgcgggaaat
     2641 tgcccagatg tggggtgaag ataacgtcag caccgaggtg tgtggcgatc tctggttgat
     2701 ggcgatgagt aaagacgcca tacggttgca ggctaacttc acaaaagctg tttgaaatgg
     2761 ccgctttacg ccctgcaccg ctcacgccgc tggtggcgtt gatcaccggc cactgattga
     2821 ggtcaagaag atcggcatca atcaacggtt tcagcgccag ctgtgccgcc gtcggataac
     2881 agcccggcac cgcaatcaaa ttcgcttctt ttaatttatt accgcaccac tccgccagac
     2941 cgtaggctgc ctgttccaac agttccgggt attgatgggt aaagccgtaa tatttttcat
     3001 agaaggtggc gtcgttaaca cgaaacgcgc cggaaaggtc gaacaccacg cagcccgctt
     3061 caagaaactg cggcgctaaa tcgtggctaa cttcatgggc ggtggcgaga aacactacgt
     3121 ccacccctgg gctaaactcg ctgatatccg acatcggctg caacggcaga tcaacgatgc
     3181 cttttagctg cggatgcaaa tcggagatta actttcccgc atcattgctt tgcgctgaaa
     3241 cagtcaaagc ggttatgttc atatgcggat ggcgatttac ataggtcact agctctgcgc
     3301 cagcgtagcc gctggcaccc acaatcagcg tattcaacat cggggctatt caccttctta
     3361 tgtctggttg ccaggttaaa cgtaaaacat tcaccttacg gctggtgggt tttattacgc
     3421 tcaacgttag tgtattttta ttcataaata ctgcatgaat attgatacta tcatgaccag
     3481 aggtgtgtca acaatgaaaa acaaattacc gccatttatc gagatttacc gcgctctgat
     3541 tgccacacct tcaataagcg ccacggaaga ggcactcgat caaagcaatg cagatttaat
     3601 cactctgctg gcggactggt ttaaagattt gggcttcaat gtggaagtgc agcctgttcc
     3661 aggaactcgc aacaaattta atatgctggc cagttgcgga cagggggctg gcggcttgtt
     3721 gctggcgggg cataccgata cggtgccatt tgatgacggt cgctggacgc gcgatccgtt
     3781 tacactgacg gagcatgacg gcaagcttta cggcttaggc accgccgaca tgaaaggctt
     3841 ttttgcgttt atccttgatg cgctacgcga tgtcgacgtc acgaaactga aaaaaccgct
     3901 ctacattctg gcgactgctg atgaagaaac cagtatggcc ggagcgcgtt attttgccga
     3961 aactaccgcc ctgcgcccgg attgcgccat cattggcgaa ccgacgtcac tacaaccggt
     4021 acgcgcacat aaaggtcata tctctaacgc catccgtatt cagggccagt cggggcactc
     4081 cagcgatcca gcacgcggag ttaacgctat cgaactaatg cacgacgcca tcgggcatat
     4141 tttgcaattg cgcgataacc tgaaagaacg ttatcactac gaagcgttta ccgtgccata
     4201 ccctacgctc aacctcgggc atattcacgg tggcgacgct tctaaccgta tttgcgcttg
     4261 ctgtgagttg catatggata ttcgtccgct gcctggcatg acactcaatg aacttaatgg
     4321 tttgctcaac gatgcattgg ctccggtgag cgaacgctgg ccgggtcgtc tgacggtcga
     4381 cgagctgcat ccgccgatcc ctggctatga atgcccaccg aatcatcaac tggttgaagt
     4441 agttgagaaa ttgctcggag caaagaccga agtggtgaac tactgtaccg aagcgccgtt
     4501 tattcaaacg ttatgcccga cgctggtgtt ggggcctggc tcaattaatc aggctcatca
     4561 acctgatgaa tatctggaaa cacggtttat caagcccacc cgcgaactga taacccaggt
     4621 aattcaccat ttttgctggc attaaaacgt aggccggata aggcgctcgc gccgcatccg
     4681 gcactgttgt caaactccag tgccgcaata atgtcggatg cgatacttgc gcatcttatc
     4741 cgacctacac ctttggtgtt acttggggcg attttttaac atttccataa gttacgctta
     4801 tttaaagcgt cgtgaattta atgacgtaaa ttcctgctat ttattcgttt gctgaagcga
     4861 tttcgcagca tttgacgtca ccgcttttac gtggctttat aaaagacgac gaaaagcaaa
     4921 gcccgagcat attcgcgcca atgcgacgtg aaggatacag ggctatcaaa cgataagatg
     4981 gggtgtctgg ggtaatatga acgaacaata ttccgcattg cgtagtaatg tcagtatgct
     5041 cggcaaagtg ctgggagaaa ccatcaagga tgcgttggga gaacacattc ttgaacgcgt
     5101 agaaactatc cgtaagttgt ccaaatcttc acgcgctggc aatgatgcta accgccagga
     5161 gttactcacc accttacaaa atttgtcgaa cgacgagctg ctgcccgttg cgcgtgcgtt
     5221 tagtcagttc ctgaacctgg ccaacaccgc cgagcaatac cacagcattt cgccgaaagg
     5281 cgaagctgcc agcaacccgg aagtgatcgc ccgcaccctg cgtaaactga aaaaccagcc
     5341 ggaactgagc gaagacacca tcaaaaaagc agtggaatcg ctgtcgctgg aactggtcct
     5401 cacggctcac cctaccgaaa ttacccgtcg tacactgatc cacaaaatgg tggaagtgaa
     5461 cgcctgctta aaacagctcg ataacaaaga tatcgctgac tacgaacaca accagctgat
     5521 gcgtcgcctg cgccagttaa tcgcccagtc atggcatacc gatgaaatcc gtaagctgcg
     5581 tccaagcccg gtagatgaag ccaaatgggg ctttgccgta gtggaaaaca gcctgtggca
     5641 aggcgtacca aattacctgc gcgaactgaa cgaacaactg gaagagaacc tcggctacaa
     5701 actgcccgtc gaatttgttc cggtccgttt tacttcgtgg atgggcggcg accgcgacgg
     5761 caacccgaac gtcactgccg atatcacccg ccacgtcctg ctactcagcc gctggaaagc
     5821 caccgatttg ttcctgaaag atattcaggt gctggtttct gaactgtcga tggttgaagc
     5881 gacccctgaa ctgctggcgc tggttggcga agaaggtgcc gcagaaccgt atcgctatct
     5941 gatgaaaaac ctgcgttctc gcctgatggc gacacaggca tggctggaag cgcgcctgaa
     6001 aggcgaagaa ctgccaaaac cagaagggct gctgacacaa aacgaagaac tgtgggaacc
     6061 gctctacgct tgctaccagt cacttcaggc gtgtggcatg ggtattatcg ccaacggcga
     6121 tctgctcgac accctgcgcc gcgtgaaatg tttcggcgta ccgctggtcc gtattgatat
     6181 ccgtcaggag agcacccgcc ataccgaagc gctgggcgag ctgacccgct acctcggtat
     6241 cggcgactac gaaagctggt cagaggccga caaacaggcg ttcctgatcc gcgaactgaa
     6301 ctccaaacgt ccgcttctgc cgcgcaactg gcaaccaagc gccgaaacgc gcgaagtgct
     6361 cgatacctgc caggtgattg ccgaagcgcc gcaaggctcc attgccgcct acgtgatctc
     6421 gatggcgaaa acgccgtccg acgtactggc tgtccacctg ctgctgaaag aagcgggtat
     6481 cgggtttgcg atgccggttg ctccgctgtt tgaaaccctc gatgatctga acaacgccaa
     6541 cgatgtcatg acccagctgc tcaatattga ctggtatcgt ggcctgattc agggcaaaca
     6601 gatggtgatg attggctatt ccgactcagc aaaagatgcg ggagtgatgg cagcttcctg
     6661 ggcgcaatat caggcacagg atgcattaat caaaacctgc gaaaaagcgg gtattgagct
     6721 gacgttgttc cacggtcgcg gcggttccat tggtcgcggc ggcgcacctg ctcatgcggc
     6781 gctgctgtca caaccgccag gaagcctgaa aggtggcctg cgcgtgactg aacagggcga
     6841 gatgatccgc tttaaatatg gtctgccaga aatcaccgtc agcagcctgt cgctgtacac
     6901 cggggcgatt ctggaagcca acctgctgcc accacctgag ccgaaagaga gctggcgtcg
     6961 cattatggat gaactgtcag tcatctcctg cgatctctac cgtggctacg tacgtgaaaa
     7021 caaagatttt gtgccttact tccgctccgc tacgccggaa caagaattgg gcaaactgcc
     7081 gttgggttca cgtccggcga aacgtcgccc aaccggcggc gtcgagtcgc tgcgcgccat
     7141 accgtggatt tttgcctgga cgcaaaaccg cctgatgctc cccgcctggc tgggtgcagg
     7201 tacggcgctg caaaaagtgg tcgaagatgg caaacagagc gagctggaag ccatgtgccg
     7261 cgattggcca ttcttctcga cgcgtctcgg catgctggag atggtcttcg ccaaagcaga
     7321 cctgtggctg gcggaatact atgaccaacg cctggtagac aaagcactgt ggccgttagg
     7381 taaagagtta cgcaacctgc aagaagaaga catcaaagtg gtgctggcga ttgccaacga
     7441 ttcccatcta atggccgatc tgccgtggat tgcagagtct attcagctac ggaatattta
     7501 caccgacccg ctgaacgtat tgcaggccga gttactgcac cgctcccgcc aggcagaaaa
     7561 agaaggccag gaaccggatc ctcgcgtcga acaagcgtta atggtcacta ttgccgggat
     7621 tgcggcaggt atgcgtaata ccggctaatc ttcctcttct gcaaaccctc gtgcttttgc
     7681 gcgagggttt tctgaaatac ttctgttcta acaccctcgt tttcaatata tttctgtctg
     7741 cattttattc aaattctgaa tataccttca gatatcctta aggaattgtc gttacattcg
     7801 gcgatatttt tttcaagaca ggttcttact atgcattcca cagaagtcca ggctaaaccc
     7861 ctttttagct ggaaagccct gggttgggca ctgctctact tttggttttt ctctactctg
     7921 ctacaggcca ttatttacat cagtggttat agtggcacca acggcattcg cgactcgctg
     7981 ttattcagct cgctgtggtt gatcccggta ttcctctttc cgaagcggat taaaattatt
     8041 gccgcagtaa tcggcgtggt gctatgggcg gcctctctgg cggcgctatg ctactacgtc
     8101 atctacgggc aggagttctc gcagagcgtt ctgtttgtga tgttcgaaac caacaccaac
     8161 gaagccagcg agtatttaag ccagtatttc agcctgaaaa ttgtgcttat cgcgctggcc
     8221 tatacggcgg tggcagttct gctgtggaca cgcctgcgcc cggtctatat tccaaagccg
     8281 tggcgttatg ttgtctcttt tgccctgctt tatggcttga ttctgcatcc gatcgccatg
     8341 aatacgttta tcaaaaacaa gccgtttgag aaaacgttgg ataacctggc ctcgcgtatg
     8401 gagcctgccg caccgtggca attcctgacc ggctattatc agtatcgtca gcaactaaac
     8461 tcgctaacaa agttactgaa tgaaaataat gccttgccgc cactggctaa tttcaaagat
     8521 gaatcgggta acgaaccgcg cactttagtg ctggtgattg gcgagtcgac ccagcgcgga
     8581 cgcatgagtc tgtacggtta tccgcgtgaa accacgccgg agctggatgc gctgcataaa
     8641 actgatccga atctgaccgt gtttaataac gtagttacgt ctcgtccgta caccattgaa
     8701 atcctgcaac aggcgctgac ctttgccaat gaaaagaacc cggatctgta tctgacgcag
     8761 ccgtcgctga tgaacatgat gaaacaggca ggttataaaa ccttctggat caccaaccag
     8821 cagacgatga ccgcccgcaa taccatgctg acggtatttt cgaagcagac cgacaaacag
     8881 ttctatatga accagcaacg tacgcagagt gcgcgtgaat acgacaccaa cgtgctgaaa
     8941 ccattccagg aagtgctgaa ggacccggcg ccgaagaaac tgatcatcgt tcatctgctg
     9001 ggtacgcata tcaaatacaa ataccgctac ccggaaaatc agggcaagtt tgatggcaat
     9061 accgatcatg ttccgcctgg attaaacgcg gaagagctgg agtcatataa cgattatgac
     9121 aacgctaacc tgtataacga tcatgtggtt gccagcctga ttaaagactt taaagcagca
     9181 aacccgaacg gtttcctggt ttatttctct gaccacggtg aagaggttta cgacacgccg
     9241 ccgcacaaaa ctcaggggcg taacgaagac aacccgacgc gccacatgta caccattccg
     9301 ttcctgctgt ggacgtcgga aaaatggcaa gcgactcatc cgcgtgattt ctcgcaggat
     9361 gttgatcgta aatacagcct ggcggaactg atccacacct ggtcagattt agcaggctta
     9421 tcttacgacg gttacgatcc aacccgttca gtggtgaatc cgcagttcaa agaaactacc
     9481 cgctggattg gtaacccgta caagaaaaac gcgctgatcg attacgacac gctgccgtat
     9541 ggcgatcagg tgggtaatca gtaaatgacc tttgtatcgc gtctggcgct atttgccgga
     9601 tgcgatactg ccgcaatgtt tacatcgaac tcccctcagg ccacactttt caggtggcct
     9661 tttttaataa tgccagttac tgttttcccg tctacatacc taagggaata acatgtatca
     9721 cgacgtcagc tatctgctct cccgcctgat caacggcccg ctgtccctgc gccagattta
     9781 ctttgccagc agtaacggcc cggtgcccga tctcgcgtat caggtcgatt ttccacggct
     9841 ggaaattgtg ctggaaggtg agtttgttga taccggcgct ggagcaacgt tagttcccgg
     9901 cgatgtgctg tacgttcctg ctggtggctg gaattttcca caatggcaag cccccgctac
     9961 tacctttagc gtgctgtttg gcaaacagca actcggcttc agcgtcgtgc aatgggatgg
    10021 caaacaatat caaaatctgg cgaagcaaca cgtcgcccgg cgcggcccac gcataggttc
    10081 ttttctgcta caaacgctca atgaaatgca aatgcagccg caggagcagc aaacggcaag
    10141 gcttatcgtc gccagcctgc ttagccactg ccgcgatttg ctcggcagcc aaatccagac
    10201 cgcctcacgc agccaggcac tatttgaagc tattcgcgat tatatcgacg aacgctatgc
    10261 ctccgcgctt acccgcgaat ctgtcgcaca ggcattctat atttcaccaa actatctctc
    10321 gcacctgttt caaaaaacgg gggccattgg ttttaacgaa tacctgaatc acacgcgact
    10381 ggagcacgct aagacgttat tgaaaggcta tgacctgaag gtaaaagaag tggcgcacgc
    10441 ctgcggcttt gtcgacagca actacttctg ccgtctgttt cgtaaaaaca ccgaacgctc
    10501 gccgtcggaa tatcgtcggc agtaccacag ccaactgaca gaaaaaccga ctactccaga
    10561 ataagatggg tttgctgcgg cgcagaaagc actttgcgca ccgcgctcat tacccgctgc
    10621 ggctcacgca ggaatgcgta gatgctgcat tgcacatagc ggcaatgttc aaatcgctcg
    10681 gcaccggcaa gctcgatatc cgtaatcaac agagcaacat ccgcccgacg gatatcctcg
    10741 tccgctaaac gattctccgt tcccagcgcg ccctgagttt caatgctgac gccccacttc
    10801 tctaactggc acaacttttc cagccgttcc gccgccatat aagtatgcgc cacgccgctt
    10861 acgcaggcgg ttactgccac caggtatgcc attttaacct cccacggtaa cctgaaatcc
    10921 ggcccgttct gccatttcgc gcatcgttgt cacatcggct gacgacggcg caggcacctc
    10981 tttcatcgac catgttttcc ccagcaggcg gtatttcggt tcgccgtact gatgaaacgg
    11041 taacagatgg atctgcctga ttttcagcgg gatcagtaca tccagcgcct gctgcatatt
    11101 ctcccggctg agcgtgaaac cagggatcaa cggtaaacgc gggatcacgt tgacaccctc
    11161 actcaccagc aaacgcagat tctccagcac gcgtggcagg ttcatcttca ccacatcccg
    11221 cgcctgagtc gcgtccataa tttttaaatc gaacagcact tcatcgcaca atttcgccag
    11281 tggtaacagc ttgctggctg gcgcgtctcc ggcagtttca atggcgcatg acacgcccca
    11341 caaccgcagc cgctgtaaaa aacgggtagc aaactccgcc tgcattaaca cttcgccgcc
    11401 agaaagcgtg acgccgccgc cggacgtgcg aaagaaaatg tcatctttca tcacttcccg
    11461 ttccagcgcg tcaaggctga tatcgcgacc aatccgttca aacgccccgg aggggcattc
    11521 atccgcatca cgcaaacatt tcgcacagtg cagacatttc gcctctctgc gtaccgtctg
    11581 gattttgccg gagatcgact ccggattagc acaccatggg caaagatgcg gacagccttt
    11641 aaaaaagacc accgtacgaa tgccctcgcc gtcattcagt gaataacgct gaatgttgaa
    11701 aatgcgcgcc acatcattgc ggcgcgtttc cacaacgtta cagctgatgc gctgtccggc
    11761 ggatgatgtc atcctggatc tccttcgaca gttcgacaaa gaaggcgctg tatccggcaa
    11821 cgcgcaccac cagcccggca taatcttgtg ggcgctgttg cgcttcccgc aacgtgtcgg
    11881 cgttcaccac gttaaactga atatgctgca acttaagctg ggtaaacgcc cgtaagaagt
    11941 cggccagttt gcgtaatccc gcttcacctt ccagggtcgc cggagtgaat ttcacgttca
    12001 gcaacgtacc gttagacagc agcgtattat cgagcttact gactgacttc agtaccgctg
    12061 ttggcccttg tgcgtcctgg cccagcatag gtgacaagcc gccgtctgcc agttgttccc
    12121 cggcaaaacg accgtctggc gtcgcgccaa ccaccgatcc caacgggacg tgagcagaaa
    12181 cggtatacga tcccggcgtg aagtaaccac cgcgcgggtt ctggtatttt tccacttctt
    12241 tgcagtagtg gcgtaacagt tcggcgctga tgttatccac ctcgtcgata tcgttaccgt
    12301 atttctcgaa gcggttaatt aagcgagcac ggactttttc gccttctggc gttgcaaagt
    12361 tggcttttaa taccgacagc aattcgtcaa aacttaaacg ctgttgctca aaaaccatcc
    12421 ctttcaacgc atggagagaa tcgctcaggt tggcgatacc gatcccctgt acgccggaga
    12481 agttataacg cgtgccgcca tcggtaatat cgcgaccttt ttccagacaa tcgctgataa
    12541 aagacgagag cagcggtaca ggtgcccagt cgcgatggcc gatatcacaa atattgctgc
    12601 cctcaaccat cagggtgatg tagtggctga tcttggcgcg gatctgctcc agtaaacctt
    12661 cataagtcag cgcagcattg ccttcatttt catgcaggca gatttccatc actttcagca
    12721 gattaaacat cgcgatgtca tgcaagccgt aggttctgcc gggaatagat aattccacac
    12781 agcccactac ggaatagtcg cgcgcatctt ccagcgaaac gccacggttg aggaacgctg
    12841 gtaccaccac ttcatcgtta aagatttgcg gaataccggt gccgaggcga atggtttcgg
    12901 cggttttcat caggaacggc gtgtcgatca aggcgttagt gcgcacgccg aggttcggtt
    12961 gcggtaattg cacgctttga taagcgtcaa ggcaaaggaa cgaaagcacg ttcaccgcgc
    13021 tacgtccgtt ctcggttaac ccgccgagca gtgcggtata gccggtcggg aaacctgcga
    13081 aataacgcgc gctgctggtg gagcgcaaca gcacgatgtc gttgcatttc acccataaag
    13141 attcgagtag ttctttcagg aacgccggat cttcgccctg ggttaaagat gcctgataga
    13201 acggcaacat atactggtcg aagcgcccca acgatagcga actggcgttg gattcgtatt
    13261 gcagaatgat gttcatgtac cagaataact ggcacgcctg ccagaacgtc tgcggcttat
    13321 gttcggcgtt atggcgggag atctccgcaa tagtcagcag ttcttcgcga cgctgggcat
    13381 ctgtgcagtt tgccgccatt gtttccgcca gttcagcgta acgcagaatg tgtttctgcg
    13441 aggcttccag cagtaacagc gcggcctgat aaaagtgatt ctccggctgt tgctgacaat
    13501 gttgctgcat ctgcgccacc agctccccca gcccgtgatt cagcaggcgt gggtaatcaa
    13561 taataatgtg cccctggcct ttatctgtct ggttgatgct gaaaatctgc gtgctggtcg
    13621 cggcttttac ttcatctgtc atctgcccgt tgatgaaatc tttcatcgaa cgtttttccc
    13681 agtacgggaa caactcttca cggtagatac gtttatcttc ttcactgata gcaaagcggt
    13741 cctgcggacg cgtcgggaat tgatccagct ctttcagcag ccagtaaggg tccatttccg
    13801 gcgacataat cccagcgcgc ggttttacgg tgcggttacc ggcaatcagt tcttcatcac
    13861 gaatcgaaat ttcaacatgt tcaaggatat acgctgttgc tttcgcccgg cgcaatatca
    13921 ccggttcgcc ttcggtttgc cgatggctgg cggtataaag cagcgcccgc tccagcgaga
    13981 tttcacgggt attggcaaac agtgcagttt tgaggcgaga gatacgattc gtcatgagga
    14041 actcctgtgg gaaagcgaac ccgcccttgc gagcgggaga agaacttaag cggtttgcgc
    14101 caggtgagct tcaattttgc tcataatggc gtcagcacgt tttactgcat cgctgatgtt
    14161 gacgcgcacg atggtcttgc cagcaaaacg ctcttcaaac ttgatgccga tatctttggt
    14221 cagaatcacc atatcagcgc tcgccacatc ttccgcagtc agttcatttt ccagaccaat
    14281 tgacccctgg gtttctactt tcacttccca gcctttcgct ttcgcggcac tttccagcgc
    14341 ctctgctgcc atataggtat gggcaacacc tgaaggacat gcggttactg caataatttt
    14401 cgtcatggct cgttcctcaa ttaattaatt tcaaaatcca aatccaggtc gtcttctttt
    14461 tcatcagtcg aagacccatt tttacgcgcc agacttttca gcacgttcac acaaacagcc
    14521 gtcaccactg ctccgacagc cactgctgcg atataaccca gcttgccttc aaccaccggc
    14581 agcacaatca gaccacccca gcctgcatag cactgcgcac cgaccagcgc cgcagttact
    14641 gcaccacaaa ctgaaccgac catgatggaa ggaatgacac gcagcggatc ggcagcggcg
    14701 aaaggaatcg ccccttcagt aacgccaaca cagcccatca ccagtgccgc tttgccggtt
    14761 tcgcgctctt ctgcggagaa atttttacga ctaatcagcg tcgccagccc catccccagc
    14821 ggcgggatac aaatacccac ggcagcgatg gccaccacgg tataaacacc ctgagcaacg
    14881 caaatcagca tgaaggcata ggccactttg ttaaccggac cgcccatatc gaacgccagc
    14941 atcagaccca tgatcaccgc cagcataaca atgctgccct gctgcatccc ctgaagccac
    15001 tgagtcaggc tgttggtcaa cgcccctaca ggctcgccca agccccacat catgatgcct
    15061 gcggtaatca gtgtgccgac gataggaata atgaagatag gcatcaccga gcgcagaact
    15121 ttatgcaccg gaattttctt caggtaatgc accacgatgc cgccgataat cccggcgatc
    15181 agtgcaccaa agaagcccgc accaaagctg ttaccaaccc aggcaccgat agcgcacgga
    15241 gccagcgcag aacgctctgc aatggaataa ccgatgtacg ctgcgaggaa aggcaccatc
    15301 agcgtcaagc ccgcaacgcc gatatcaaac agttttttca gatttggatc ggctacggca
    15361 tccggcactg cgcctttgcc atacaacatg acggaaaccg ccagcaaaat accgcccgat
    15421 accacgaagg gaatcatgtg tgaaacgccc gtcattaaat gctgacgggt atttttcagg
    15481 atctgcacca actcattcat aagctgctcc cgggcttgcg aaagcccatg tccatatttt
    15541 tgatggagga aacaataggc aatcgcaggc ggagcaaaca gtggataaaa aggccattta
    15601 ctggactttt gtaatatcag tacaaaaatg cgatccgcct cataacttgc gataaaccgg
    15661 aagcatgccg caaaacagcg tcttgtgaga cgcgtcgcaa attgacggtt acattacatt
    15721 tgtccagctt ttggcagatt tgtcacatgg caggccgttg ccgtgcgctc ttatcctgta
    15781 aacagaaaac cgtaccggga gagaggcaat ggccctgatt gtggaattta tttgtgagct
    15841 acctaacggc gtacatgcac gtccggcaag ccacgttgaa acgctgtgta atactttttc
    15901 atcacaaatt gagtggcata acctgcgcac tgaccgcaag ggcaacgcca aaagcgccct
    15961 tgcgctgatt ggcaccgata cgctggcggg cgataactgc cagttactga tttccggggc
    16021 cgacgaacag gaagcgcacc agcgtttaag ccaatggctg cgcgatgaat tcccccactg
    16081 cgatgcgccg ctggcggaag ttaaatctga cgaactggaa ccactgccgg tttcactgac
    16141 caatctgaat ccgcaaatta tccgcgcccg caccgtgtgc agcggtagtg caggcggcat
    16201 tctgacgccg atctcttctt tagatctcaa tgcgctgggt aatcttcccg cagccaaagg
    16261 cgttgacgcc gagcaatccg cactggaaaa cggcctgacg ctggtactga aaaacattga
    16321 gtttcgtctg ctggatagcg acggtgccac cagcgcgatt ctggaagcgc accgatccct
    16381 ggctggcgat acttccctgc gcgaacattt actggcaggc gtcagcgccg gattaagctg
    16441 cgccgaagcg attgtcacca gtgcgaatca cttttgcgaa gagttcgccc gttccagcag
    16501 cagctacctg caagaacgtg ccctggacgt acgcgacgtc tgcttccagt tactccagca
    16561 aatctacggt gagcaacgct tcccggcacc gggcaaactg acgcagcccg ccatttgtat
    16621 ggctgatgaa ctgactccca gccagttcct cgaactggat aaaaatcacc tcaaaggtct
    16681 gttactgaaa agtggcggca ccacctcaca taccgtaatt cttgcccgtt cgttcaacat
    16741 tccgacactg gttggtgtag atattgatgc ccttactccg tggcagcatc aaacgattta
    16801 tatcgacggc aacgctgggg cgattgtggt tgagccagga gaagccgtag ctcgttatta
    16861 tcagcaagaa gcccgcgtac aggacgccct gcgtgagcaa cagcgtgtct ggctgaccca
    16921 acaagcccgt accgctgacg gtatccgcat tgaaattgcc gctaacatcg ctcactccgt
    16981 ggaagcgcag gccgcattcg gcaatggtgc ggaaggcgtt ggtttgttcc gcactgaaat
    17041 gctctatatg gatcgcacca gtgcaccggg cgaaagcgag ctgtacaaca ttttttgtca
    17101 ggcgctggag tctgccaacg gacgcagcat tattgtgcgc actatggaca ttggcggcga
    17161 caaacccgtt gattatctga acattccggc agaagctaac ccgttcctcg gttatcgcgc
    17221 tgtgcgtatt tatgaagaat acgcctcgct gttcaccaca caactacggt cgatcctgcg
    17281 cgcctccgct cacggcagcc tgaaaatcat gatcccgatg atctcctcaa tggaagagat
    17341 cttatgggtg aaagaaaaac tggcggaagc caaacagcaa ctgcgtaacg aacacattcc
    17401 gtttgatgag aagatccagc ttggcattat gctggaagta ccgtcggtga tgttcatcat
    17461 cgatcaatgc tgcgaagaga ttgatttctt tagtattggt agtaatgacc tgacgcaata
    17521 tctgctggca gtggatcgcg ataacgctaa ggttactcgt cactacaaca gcctgaatcc
    17581 ggcattcttg cgggcgctcg attacgccgt gcaggcggtg catcgccagg gcaaatggat
    17641 tggtctgtgc ggtgagctgg gagcgaaagg ttccgtgctg ccgttgctgg tcggcttagg
    17701 gctggatgaa ctcagcatga gcgcaccatc aattccggcg gcgaaagcgc ggatggcgca
    17761 acttgatagc cgtgagtgcc gcaagttgct caaccaggca atggcctgcc gtacttcgct
    17821 ggaagtggaa cacctgctgg cgcaattccg catgacccaa caggacgcac cgctggtcac
    17881 cgccgagtgc atcacgctgg aaagtgactg gcgcagcaaa gaagaagtgc tcaaaggcat
    17941 gaccgataac ctgctgctgg cgggccgctg ccgctatccg cgtaaactgg aagccgactt
    18001 atgggcgcgc gaggccgttt tctctaccgg tctgggcttt agttttgcca ttccacacag
    18061 caaatcagaa cacattgagc aatccaccat cagcgtggcg cgtctgcaag cgccggtgcg
    18121 ctggggcgat gatgaagcgc aattcatcat tatgttaacc ctgaacaaac acgctgcggg
    18181 cgatcagcac atgcgcattt tctcgcgcct cgctcgccgc atcatgcacg aagaattccg
    18241 taacgcgctg gttaacgccg cctctgccga cgctatcgcc agcctgctgc aacatgaact
    18301 ggaactgtaa aaggaaacat catggaactg tatctggaca ccgctaacgt cgcagaagtc
    18361 gaacgtctgg cacgcatatt ccccattgcc ggggtgacaa ctaacccgag cattatcgct
    18421 gccagcaagg agtccatatg ggaagttctg ccgcgtctgc aaaaagcgat tggtgatgag
    18481 ggcattctgt ttgctcagac tatgagtcgt gacgcgcagg gtatggtgga agaagcgaag
    18541 cgcctgcgcg acgccattcc gggcattgtg gtgaaaatcc cggtgacttc cgaaggtctg
    18601 gccgccatta agatgctgaa aaaagagggt attactacgc ttggcaccgc agtgtacagt
    18661 gctgcacaag ggttactggc cgcgctggca ggggcaaaat acgttgctcc gtatgttaac
    18721 cgcgtagatg cccagggcgg ggacggcatt cgtacggttc aggagctgca agcgctgtta
    18781 gaaatgcacg caccagaaag catggtgctg gcggccagct ttaaaacgcc gcgtcaggcg
    18841 ctggactgtt tactggcagg atgtgaatcc atcaccctgc ccttagatgt agcgcaacaa
    18901 atgctcaaca cccctgcggt agagtcagct atagagaagt tcgaacacga ctggaatgcc
    18961 gcatttggca ctactcatct ctaaaggagc aattatggac cgcattattc aatcaccggg
    19021 taaatacatc cagggcgctg atgtgattaa tcgtctgggc gaatacctga agccgctggc
    19081 agaacgctgg ttagtggtgg gtgacaaatt tgttttaggt tttgctcaat ccactgtcga
    19141 gaaaagcttt aaagatgctg gactggtagt agaaattgcg ccgtttggcg gtgaatgttc
    19201 gcaaaatgag atcgaccgtc tgcgtggcat cgcggagact gcgcagtgtg gcgcaattct
    19261 cggtatcggt ggcggaaaaa ccctcgatac tgccaaagca ctggcacatt tcatgggtgt
    19321 tccggtagcg atcgcaccga ctatcgcctc taccgatgca ccgtgcagcg cattgtctgt
    19381 tatctacacc gatgagggtg agtttgaccg ctatctgctg ttgccaaata acccgaatat
    19441 ggtcattgtc gacaccaaaa tcgtcgcggg cgcacctgca cgtctgttag cagcgggtat
    19501 cggcgatgcg ctggcaacct ggtttgaagc gcgtgcttgc tctcgtagcg gcgcgaccac
    19561 catggcgggc ggcaagtgca cccaggcggc gctggcactg gctgaactgt gctacaacac
    19621 cctgctggaa gaaggcgaaa aagcgatgct tgccgccgaa cagcatgtag taaccccggc
    19681 gctggagcgc gtgattgaag cgaacaccta tttgagcggc gttggttttg aaagcggcgg
    19741 gctggcggcg gctcacgcgg tgcataacgg cctgaccgct atcccggacg cgcatcacta
    19801 ttatcacggt gaaaaagtgg catttggtac gctgacgcag ctggttctgg aaaacgcacc
    19861 ggttgaggaa atcgaaaccg tagctgcact tagccatgcg gtaggtttgc caataactct
    19921 cgctcaactg gatattaaag aagatgtccc ggcgaaaatg cgaattgtgg cagaagcggc
    19981 atgtgcagaa ggtgaaacca ttcacaacat gcctggcggc gcgacgccag atcaggttta
    20041 cgccgctctg ctggtagccg accagtacgg tcagcgtttc ctgcaagagt gggaataacc
    20101 tactccaaac tcccggcttg tccgggagtt tgaacgcaaa attgcctgat gcgctacgtt
    20161 tatcaagcct acgaaatctc tgcaatatat tgaatatgcg tgcttttgta ggccggataa
    20221 ggcgttcact ccgcatccgg cactctgtgc cagtaatctg aaactcccac gttgttcagg
    20281 agttttttat ccgcttctgg acattttctc cacagaacaa tcgttagccc ggaaggtcga
    20341 atctgtgact cttattttca cggacgaacc agatgaaagc ttcactggcg ctgctcagcc
    20401 tgctaacggc atttaccagc cactccttaa aatctcccgc cgttccacca accgtggtac
    20461 aaatccaggc caataccaac cttgccatcg cagacggtgc cagacagcaa atcggcagca
    20521 cgctatttta cgatccggcg tatatgcagc ttacctatcc tggcggtgat gtcccgcaag
    20581 aacgcggggt atgttccgat gtggtgatcc gcgccctgcg cagccagaag gtcgatttgc
    20641 agaaactggt tcacgaagat atggcgaaga attttgccga gtacccgcaa aaatggaagt
    20701 taaagcgccc ggacagcaac atcgatcacc gtcgggtgcc taatctggaa acctggttta
    20761 gccgccacaa caaaacacgc cccatcagta aaaatgccgg tgactatcaa gcgggcgata
    20821 ttgtctcctg gcgattagat aacgggctgg cgcatattgg ggtggtgtca gatggcttcg
    20881 cccgcgacgg tacgccgctg gtgatacata acattggcgc aggcgcgcag gaagaagatg
    20941 tactgtttag ctggcggatg gtggggcatt atcggtattt tgttaaataa gcgatcgccc
    21001 cacatgaaga aaaaattcag atgcttttta cactgacagc ttcttttttc ttacggctca
    21061 ccagcgccag tgccagcaca atcagcacaa taccgctacc ttcaaccacg ccaggattct
    21121 cgccgagcag ccaccaggaa aagagtacgc cgcaaacggg aacggccagt gtgcttaagc
    21181 tggcaatact ggcaggcaag tttttcaata caaacaacca taagctccac gccagcgccg
    21241 tcgccagaat cgcactgtag accagcgccc agaacacagt gggctgccag tcaatttcac
    21301 gttgtggcac cagtaaagcg accacactca tcaccagcgc cgcatacagc atctgccagg
    21361 atgttaacga caataaatcc acgcgcggat gacgggcata cagacgttta gcaacaatcg
    21421 cgctcgcccc ccaactaaca ccggagagaa ttgccagcat ggcacttttc atcgaagaga
    21481 aatcgagttg ccacggctgc aacaccaaaa ataagccgaa agcggcaatc agtatcgcga
    21541 aatattgccc acgtcgcagg cgttcaccga gaaacaacgc ggcgaaaatc accacccaga
    21601 acggcatggt atagctcagg atcgccacct tccccgcacc tccgctgacc aacgcccatt
    21661 gtgccaggcc aatcattccg caggtttgta acagggcaat agctaaggtg tatttaaacg
    21721 gcgtcgggcg cattgcgcga ccacgtaata aaaggacgat gaataaaacg agagcgccga
    21781 aaatgcagcg taaggcggtg aagtcgaagg caccgatgta actggtgact tgcttcatga
    21841 aaatccagct ataactccag ataagtgtga gcacaaccag gccactgatt gccagtgggt
    21901 tgctctttcc tgcggcagac atgagagatc cagtgtgtag ttttcctgtg gacactatac
    21961 gccggtaaag aaacttcagc gactgcctga aagacagtcg ctgaagaagg tcagattaca
    22021 gcaggtcgaa acggtcgagg ttcatcactt tcacccatgc cgccacgaag tctttaacaa
    22081 acttctcgtg ggcatcgctg ctggcgtaaa cttccgctac cgcacgcagg acagagttag
    22141 aaccaaatac cagatccgca cggctggctg tgtatttcac ttcgccggtt tcacggtcac
    22201 gaccttcgaa cagctctttc gattcgtcag tcgctttcca ctcgtaacgc atatccagca
    22261 agttcacgaa gaagtcattg ctcaatacgc caacgcggtc agtgaagacg ccgtttttgc
    22321 tgccatcgaa gttggcaccc agtacacgca tcccgcccac cagcgcagtc atttccggcg
    22381 cggtcagcgt cagttgctgt gctttgtcga tcagcagtga ctcggtggtg gaaacgtcca
    22441 gacgagcgcg atagttacgg aaaccgtcag caattggctc cagcagctca aacatctcaa
    22501 tgtcagtctg atcctgacgc gcatcaacgc gacccggcgc aaacggtaca tgaatgctca
    22561 aacctgcggc gcttgcggct ttctcaacac caaccacacc agccagcacg atgatatccg
    22621 ccagcgaggc tttaccagac tctttctgga ttttctccag aacaggcaga gcacgaacgg
    22681 ctgcggcgtt cacatcccag tcgcgctgcg gcattaatgc cagacgcgca ccgttggcac
    22741 caccgcgttt gtcgccacca cggaaggtag aagcagatgc ccaggccacc gataccagct
    22801 cactaacaga cagaccagaa tccgcaatcg cgaatttcag atcgataatg tcctgctcgg
    22861 tcgggttgta gatcggctgc ggcagcggat cttgccagat cagatcttct ttcggtactt
    22921 ccggtccgat gtaacgagat ttcggcccca tatccctgtg cgtcagtttg aaccaggcac
    22981 gggcaaaggc ttcgttgaac gcctgcggat cgttgaggaa acgacgagag atcttctcga
    23041 actcaggatc aaaacgcagc gtcaggtcgg tcaccagcat cgtcggttta cgtttcttcg
    23101 acggatcgaa cggatccggg ataatttccg gtgcgtctac cgcttcgaac tggattgcgc
    23161 cagccggact gcgggtctgt acccactcat atttgaacag gttctcgaag aaatagttgc
    23221 tccactgggt cggcgtctgg gtccagacaa cttccagacc agaggtaatg gcatctgcgc
    23281 caacgccgct gccgtaagtg ctcgcccagc ctaaaccttg ttcttcaatc ggtgcagctt
    23341 ctggatcagg acctacattt gatgtcggac cggcaccgtg ggttttaccc agcgtatgac
    23401 cacccgcaat cagcgccacg gtttcttcgt cgttcatgcc catgttgccg aaggtcgcgc
    23461 ggatagctgc tgccgcagaa agcggttcgc cgctgtggtc cgggccttcc gggttaacgt
    23521 aaatcaggcc catctcggtt gcacccagcg gtgctttcgc cagcgcttcc ggatgacggt
    23581 gagtcagcca ggctttttca tcaccccagt taacatccag atccggttcc cagacgtctt
    23641 cacgaccggc accaaaaccg aaggtacgga agccggagtt ttccagcgcc acgttacctg
    23701 cgaggataaa caggtcggcc caggagattt tctgaccata tttctgtttg attggccaca
    23761 acaggcgacg cgctttatcg aggcttacgt tatccggcca ggagttcagc ggtgcaaaac
    23821 gttgctgacc acgacccgcg ccgccgcgtc catcgattga acggtaagta cccgcgccgt
    23881 gccaggccat acgaataaac agaccggcgt aactgcccca gtcggcaggc caccacggct
    23941 gagattctgt cagcagggct ttcagatctt ttttcaggcc gtagtaatct aatttgctga
    24001 attctttgcg gtagtcaaag tcctcaccca gtgggttaga acgattagaa tgttggttta
    24061 acaggtcaac acggagttga tttggccacc agtcgcgagt ggttgtgccc gcccccgcgc
    24121 tctggtcgtg accgccctga tggaacgggc atttgccagt ggctgtggtg ttatgaatat
    24181 cgtctgacgt gctcatcaat gtgctcccct ctacagtgtt accgttacga tacacagcgt
    24241 tagagagaag ttataattga attaatccac ggatgcgata gttgagatct tacatattgt
    24301 tggttaaaga gatgtagatc aaattgatcg taattaagct tttttgatta tgtccgaatt
    24361 cggacaaatg ctttataaaa aagggtttta ggttcatatc cttcaagtaa tgagggagaa
    24421 aacaaggctg ataaccttat tttcccgccc tcatttcgag gcagcatttt gtgctctgtt
    24481 taaaatttgt gatcactgtg tgattttcac aaaagccaca ctatttataa accaggtcga
    24541 acacccagcg tatggcaaat cgcgtaactc atttcagcgc ggttaagcgt atagaagtgg
    24601 aaatctttca ctccttcacg gcttaaaatc ttcaccatat ccatggcaat attcgctccc
    24661 accagtttgc gggtttcagc atcgtcatcc agcccgtcaa acatttgtgc catccacgcc
    24721 ggaatacgca cgttggtcat atcggcaaat ttcttcgcct gtttaaagtt agataccggc
    24781 aaaatccccg gaataatttc cacatcaatg cccgccgata cgcagcggtc acgaaaacgc
    24841 aggtagcttt cgacatcaaa gaagaactga gtaatcgcgc ggttggctcc ggcatcgact
    24901 ttgcgtttca ggttgagcaa atccgcctga gcgctttttg cttccgggtg aacttccgga
    24961 tacgccgcca cggagatatc gaaatctgcc acttctttta acagcgtcac caggtcagaa
    25021 gcatacattt ccggcttacc acttcccggc ggcagatcgc cacgcagcgc cacgatatga
    25081 cgaataccgt tattccagta gtcgcgtgca atggtgcgca gctcgtcggg cgtcgcatca
    25141 atgcaagtaa gatgcggtgc cgcttccaga ccagtgcgat ctttaatgcc tttaataatg
    25201 ctgtgcgtac ggtcgcgctc gccggagttc gcgccatagg tcaccgatac aaacttcggt
    25261 ttcaggctgc taaggcgatc gatggagttc cacagggtct gctccatttc actggtacgc
    25321 ggcgggaaaa actcgaacga aacgttaatc tgcccctgga cttctgccag gctctgattc
    25381 agggcatccc gctggctggc gtgaaaaaag ctcatacctt acctcatcaa tcgcttgtag
    25441 ttatatgttg tgttgtgcac atctatccat ttagacgtcc agatgtaaag atgaaggaaa
    25501 agccgaaggg cgtcaaccga aaaatgacca atgatggtga ggaaaaatca gggaagatga
    25561 aaaaacttca ggatggtaag aaaaagaaaa tccggtatgc aaagcaaacc ggactgcaaa
    25621 tataaactaa ccatgtactt attgcttctt gccggattcg tcgtaaacgc ttcatccggc
    25681 ctaatcctat gataattcaa tatatttcat tagaaaccat agacccgaaa acatgagtac
    25741 cgggcattat taaatttctg aaattacaac aactgtgcca gccggttgat atccgactga
    25801 atcgccccgg cggtgacgtc gcgcccagcg ccaggcccac ggatcaccag cggattatcg
    25861 cggtaccagc ggctttcgat ggcaaagacg ttatcgcatg gcagcagtga tgccagcgga
    25921 tggtcttcac gcaccgcttc cacgcccaca cgcgctttgc cattggcatc gaaacgcgcc
    25981 acgtagcgca gcaccagccc catttcgcgg gccgcttcca gccgttgcac catctgctcg
    26041 ttaagttcat cgccattttc aaagaaatgg tcgatgctgc cgccttcgca atgagcaggc
    26101 accagcgatt ccacacgtac ctggtccggt tcaatgttgt aacccgcttc acgcgccaga
    26161 attaccagct tgcgcattac gtctttgccg gagaggtcgt cacgcggatc aggctcggtt
    26221 aagccctgct gccacgcctg atccaccagc tcggtaaacg gcacgctacc gtcgaattgc
    26281 aggaacagcc aggagagcgt gccggagaag atcccgctga tcgacaaaat agtatcgccg
    26341 ctgtcgatca gatcgcgcac ggtgtggttg atcggcaagc ccgcaccgac ggtggcattg
    26401 tacagccagt gacgcccggt tttttcgaag gcgtcgtgga tctggcgata tttattgctg
    26461 tcgctggctc ccgccagttt gttggcgctg ataacgtgga aaccgtggct ggcgaaatca
    26521 agatactgat cagcaagctg ctggctggcg gtaacgtcca gcaccactaa atcatcatac
    26581 ggatgggcgc gcatccacag gaacaacgac tcttcatcct gctcaaccgc ttcatcgttg
    26641 aagaaggcta acgcgcggct ggcgtccagc ccgtcatagc tcaacaggct gcggcggctg
    26701 tccaccacac ctgccagcac aaactcaaag ccggtacgtg ccgaaagcgt gctctgctca
    26761 cgggcgaaca gttccagcca acgggaaccg atattgccct taccgaacaa taccaggccg
    26821 atgcgttttt ctgcgcggaa gacggactga tgcagcccct gaatcaggct ttcggtcggg
    26881 ccggtgcgca gtactgccac caggctgatg ccgtcatcgg actgccaggt aaattcgacc
    26941 ggctggcctt tcagttgctg ccagaagcgg tggcaatgca gcgggttacg ggtgacgcct
    27001 gcaccgacca tcgccaccag cgccagcccc tgacgcaggc gcagttcgcc aggtaatccc
    27061 gcttcgtcga ggattttcag cgcactgtcg gccacttctg aggtgtagca aaattgcagc
    27121 aactggcgat cgttatgtac gccaaccgcc agcgggcgta cctgcgcgcg tttcaggatt
    27181 tggtcgatct ctttatgcgc cagtttgaaa tcctgactgg cgggcacctg aaactcaatc
    27241 aaacagacat catcgtggct ggtgacaata cgcgcaccag taccggaggc cagcacgcgt
    27301 tcaatgcgcg tggaaccttg atccggcgtg tagctacagc gcagttgcag gtcgatttcg
    27361 ctgccagaaa ccggctgtaa agtacgggcg tgaagaacgg gagccgccag gcgcgccagt
    27421 tcgctggcct catccagacg cagcaacggc agcaggcagg catctttcac tttacgcggg
    27481 tcggcactgt ataccccggc gacgtcgctc cagatggtta cgcgagaaac acccgccagc
    27541 gcaccgattt gtgtcgcgga atagtcggaa ccgttacgcc ccagcagcac cgtttcaccg
    27601 gcgttgttgc ggctgataaa tccggtcacc accagacgtt tgcccggatg ttgcaccagc
    27661 agctgttgca gcaacgggta agaaagccct tcatcaacct gcggttgtgc ggcgcgttca
    27721 gcgcgtaaaa actcgcgggc atcaagccag gccgctggca gcccttgttg attaagtacc
    27781 gcagacatca ggcgtgccga ccacacttca ccgtgcccca ccacttccgc atacactgcg
    27841 tcgttaatac cgctgtcgag cagcgccgcc aggcgctcaa ggtcgctgac aaaagcgcta
    27901 atgaggctat cggcttcttc agcgggtagc agaccgctaa tcagatcgca ctgatagcga
    27961 cgcagcgttt gttgaacctg atgcgcagag agacgatcgg tctggcttag tttcaaccag
    28021 ttaatcaact ggttagtggt gctaccggcg gcggaaacca ccatcatatc gtcaggctga
    28081 gagtactccg ccataatgcc cgcgacacgc aaataacact tcacatcagc cagactactg
    28141 ccaccaaatt tatgcagctg acgacctttc gcccctgcct gcgcaatcac actcattttt
    28201 accccttgtt tgcagcccgg aagccatttt ccaggtcggc aattaaatct tcgccatctt
    28261 caataccggt ggagatacgc agcagcgtct cggagatccc ggcggcagca cgcgcttctg
    28321 gtgccatgcc tgcatgtgtc atggttgcgg cgtgagagat taaactttcc actcccccta
    28381 atgattccgc cagcgtaaac aacgacagcc cgctcaggaa acgacgcagc gtctgctcat
    28441 cgccatccag ttcaaaactc aacattgcgc caaagccttt ttgctgacgc gcggcaattt
    28501 catgcccctg attttccggc agcgacgggt gatacagttt tttcaccaac ggctgggttt
    28561 gcagatattt cacaatcgcc tgcgcgttac gctgggccaa ctccatgcgc ggcaccagcg
    28621 ttcgcaaccc gcgtagtagc agatagctgt caaacgcgcc gcccgtcacg ccaatattgt
    28681 ttgcccacca ggcaagttca gtgacaacgt ccgggtcttt agcaatcacc acgccggcca
    28741 ccacgtctga gtgaccgttc agatatttcg tgcatgaatg caacaccaga tcggcaccta
    28801 atgccagcgg attttgtaat gccgggctta agaaggtgtt atccaccacg ctcaccgccc
    28861 cgacttccct tgccagatgg cagattttcg caatatccac gacgcgtaac aatggattac
    28921 ttgggctttc taccagtacc agtttgggtt tttctgccag cgctgcccgt aatgcctgtt
    28981 catcgccttg atcaacaaac aacacgcgat agcaaccacg tttcgccagg ctgtcgaaca
    29041 ggcgatagct accgccgtag cagtcgtgcg gcgctaccag cagatcgcca ggtttcaaaa
    29101 agacggtcgt taccaggtga atcgcggaca tgccggtatt agtaagtact gcaccagcac
    29161 cgccttccag ttctgccagt gctcgctgaa ccacatcgcg cgttgggttg ccacgacgcg
    29221 agtaatcatg cgcgcgcggt tcattaaatc cggtaaagtt ataggtactg gaaagatgga
    29281 tcggtgggac aacgcaacca tactgttcgt cgtcatttaa cccgctacgc actgcgatgg
    29341 tggcctgttt acgcgtcatg tgatgaagtt ccctgggctt ggtcggtgaa atgtcaggca
    29401 ccagagtaaa cattgtgtta atggacgtca atacatctag acatctaaac ttctttgcgt
    29461 atagattgag caaatcccaa atagccgtta aaattatatg cattatcacg ccgacaggtg
    29521 cattacacga tgtcacggta acgcctgtac ggtaaactat gcgggtttac ggtcagtacc
    29581 cacataaatt gtgtggtctg gtctcaattt attgacgaag aggattaagt atctcatggc
    29641 tgaatggagc ggcgaatata tcagcccata cgctgagcac ggcaagaaga gtgaacaagt
    29701 caaaaagatt acggtttcca ttcctcttaa ggtgttaaaa atcctcaccg atgaacgcac
    29761 gcgtcgtcag gtgaacaacc tgcgtcacgc taccaacagc gagctgctgt gcgaagcgtt
    29821 tctgcatgcc tttaccgggc aacctctgcc ggatgatgcc gatctgcgta aagagcgcag
    29881 cgacgaaatc ccggaagcgg caaaagagat catgcgtgag atggggatta acccggagac
    29941 gtgggaatac taaagcaaaa aagagcggcg cggagtggaa tcgcctgatg cgctacgctt
    30001 atcaggccta cgtcatattg caatttattg aatttgcacg aacttgtagg ccggataagg
    30061 cgttcacgcc gcatccggca taaacaacga gcacgttgtc tgcgacccac cgctttttat
    30121 acatggacgt ttaactatga aaaacaggct gctgatcctc agcctgctgg tttctgtacc
    30181 tgcctttgcc tggcagccgc aaaccggcga catcatcttt cagatctctc gctcatcgca
    30241 aagtaaagcg atccaactgg cgacccattc cgattacagc cacaccggta tgctggtgat
    30301 acgcaacaaa aagccctacg tttttgaagc ggtcggcccg gtaaaataca ccccgctcaa
    30361 gcagtggatc gcccatggtg aaaagggtaa gtacgttgtt cgccgcgttg aaggcggact
    30421 gagtgttgaa caacagcaaa aactggcgca aacggcaaaa cgttatctcg gtaaaccgta
    30481 tgatttcagc ttctcatgga gcgacgatcg ccagtactgt tcggaagtgg tgtggaaggt
    30541 ttaccagaac gcgctgggaa tgcgcgtggg cgagcagcag aagttgaaag agttcgatct
    30601 cagcaacccg ctggtccagg cgaagctcaa agaacgctac ggcaaaaata ttccgcttga
    30661 ggaaacggta gtttcaccac aagccgtttt cgacgcgcca caactcacca cggttgccaa
    30721 agagtggccg ctgttttcgt ggtaatggat gatgaaaaaa ataattcccg ccgtagtctg
    30781 tttgttgctt tgtagttgca cgacctttcc gcaagctgtc gctccggtaa acggtgatgc
    30841 ctccaattag ttgaattaag gatataatac tcgcttttaa ggttgtttca tggtcacagt
    30901 taaccttcat tgcccccgtt gccagtccgt tcaggtttac cgtcacgggc agaacccaaa
    30961 aagacatgat cgattccgcg gccgtgactg tcaccgggta tttcagctca gttactgtta
    31021 cgagccccgt aaaccgggag ttaaagacca aatcgctgaa atgactttca acggtgccgg
    31081 ggttcgcgat actgcccgta cgctgaaaat cggcattaat acagtcatcc gaactttaaa
    31141 aaactctcgc cacgaagaat aacgtcatcc ccggtcgctc atgctgatgt ggcactcatc
    31201 tgcaaactta gtcagcaatg gagctttgtc ggcagtaaag cgcgacagca ctggctctgg
    31261 tacgtgtaca acacgaaaac tggtggtgtt ctggcctaca cttttggccc ccgggctgac
    31321 gaaacctgtc gtgaattgcg ggcgctgctt acccttttac catcggcatg ttgaccactg
    31381 acgaatgggg tagttacgct agagagttac cgaaggagaa gcatctgact ggtaagattt
    31441 ttactcagca cattgagcgt aacaatctga ccctgagaac ccgaatcaaa cgtctggccc
    31501 gcag
//
LOCUS       DAFNBJ010000046        31248 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000046 DAFNBJ010000000
VERSION     DAFNBJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.046"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            132..3884
                     /gene="yfaL"
                     /locus_tag="L2X49_001942"
     CDS             132..3884
                     /gene="yfaL"
                     /locus_tag="L2X49_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBN4797031.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVTDSCQGYDVKASCQ
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIVNNTAFTNNTAEGYGGAIYTNSVTAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITSDVDTRSDAAGHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKMVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYA
                     WFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4012..4734)
                     /gene="ubiG"
                     /locus_tag="L2X49_001943"
     CDS             complement(4012..4734)
                     /gene="ubiG"
                     /locus_tag="L2X49_001943"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HBN4797032.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEKHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4881..7508
                     /gene="gyrA"
                     /locus_tag="L2X49_001944"
     CDS             4881..7508
                     /gene="gyrA"
                     /locus_tag="L2X49_001944"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBN4797033.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7657..9345
                     /locus_tag="L2X49_001945"
     CDS             7657..9345
                     /locus_tag="L2X49_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HBN4797034.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARWLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGIAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9342..9965
                     /locus_tag="L2X49_001946"
     CDS             9342..9965
                     /locus_tag="L2X49_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBN4797035.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9989..14503
                     /locus_tag="L2X49_001947"
     CDS             9989..14503
                     /locus_tag="L2X49_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBN4797036.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPFEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALAKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQVNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQAIGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLITGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEIARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYMRSYAPAQQSVAA
                     GSEWTRMQVK"
     gene            14504..16153
                     /locus_tag="L2X49_001948"
     CDS             14504..16153
                     /locus_tag="L2X49_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBN4797037.1"
                     /translation="MNWRRIVWLLALVTLPTLAEEPPLQLALRGAQHDQLYKLSSSGV
                     TNVSTLPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMAAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQAETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16158..16934
                     /locus_tag="L2X49_001949"
     CDS             16158..16934
                     /locus_tag="L2X49_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBN4797038.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPVEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(17008..18192)
                     /gene="atoB"
                     /locus_tag="L2X49_001950"
     CDS             complement(17008..18192)
                     /gene="atoB"
                     /locus_tag="L2X49_001950"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HBN4797039.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SQHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18223..19545)
                     /locus_tag="L2X49_001951"
     CDS             complement(18223..19545)
                     /locus_tag="L2X49_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HBN4797040.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19542..20192)
                     /gene="atoA"
                     /locus_tag="L2X49_001952"
     CDS             complement(19542..20192)
                     /gene="atoA"
                     /locus_tag="L2X49_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HBN4797041.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     ADEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20192..20854)
                     /gene="atoD"
                     /locus_tag="L2X49_001953"
     CDS             complement(20192..20854)
                     /gene="atoD"
                     /locus_tag="L2X49_001953"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HBN4797042.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21050..22435)
                     /gene="atoC"
                     /locus_tag="L2X49_001954"
     CDS             complement(21050..22435)
                     /gene="atoC"
                     /locus_tag="L2X49_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HBN4797043.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETNCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEISHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIGATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22432..24258)
                     /gene="atoS"
                     /locus_tag="L2X49_001955"
     CDS             complement(22432..24258)
                     /gene="atoS"
                     /locus_tag="L2X49_001955"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HBN4797044.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24425..27274
                     /gene="rcsC"
                     /locus_tag="L2X49_001956"
     CDS             24425..27274
                     /gene="rcsC"
                     /locus_tag="L2X49_001956"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBN4797045.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIHQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLSVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27474..28124)
                     /gene="rcsB"
                     /locus_tag="L2X49_001957"
     CDS             complement(27474..28124)
                     /gene="rcsB"
                     /locus_tag="L2X49_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBN4797046.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28141..30813)
                     /gene="rcsD"
                     /locus_tag="L2X49_001958"
     CDS             complement(28141..30813)
                     /gene="rcsD"
                     /locus_tag="L2X49_001958"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBN4797047.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEQDATATGNNDNEKEGTDSVSIHFNS
                     TKIEIASALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         7660 a         7675 c         8381 g         7532 t
ORIGIN      
        1 actgatatta ttcatcaaat aataacaaaa tagccattgc accgggttga acaatttaca
       61 aaagaaagat attccatatc gtataatgcg attaaatacg ccgtcttata gaaaatgatg
      121 gttagattaa tatgcggatt atctttctac gcaaggagta tttatcttta ctcccgtcaa
      181 tgattgcatc tcttttctct gctaacggtg tcgcggcggt cactgattca tgccagggat
      241 atgatgtcaa agcgagttgt caggccagca ggcaaagcct ttcaggcatt acgcaggact
      301 ggagtatcgc tgatgggcaa tggctggttt tttcggatat gaccaataac gccagcggtg
      361 gggccgtatt tttgcaacaa ggagcggaat tttcactatt accagaaaat gaaactggaa
      421 tgactctgtt tgccaataac accgttacag gagaatataa taacggcggg gccatatttg
      481 ctaaagaaaa ctcaacgctg aatcttactg atgttatttt ttccggtaac gtcgcaggcg
      541 gctatggtgg cgcaatctat tcttctggta ctaacgatac tggtgccgtc gatttacgtg
      601 tcactaacgc catgtttcgc aataacatcg ctaatgatgg caaaggtggc gcaatttata
      661 ccattaataa tgacgtttat ttaagtgatg ttatttttga taacaaccag gcatatacat
      721 caacaagtta cagtgatggc gatggcgggg caatcgatgt taccgataat aatagcgaca
      781 gcaagcatcc ttcaggttat acgatagtaa ataacactgc ctttacaaat aacactgccg
      841 aaggttatgg cggggcgata tataccaata gcgtgacggc tccctatctt attgatattt
      901 ctgttgatga cagctacagc cagaacggag gcgtgttagt cgatgagaac aatagcgcag
      961 caggctatgg agatggtcct tcctctgcgg cgggtggctt tatgtatctc ggcttaagtg
     1021 aagttacctt tgatattgcc gacggaaaaa cgctggttat tggcaataca gagaatgacg
     1081 gagctgttga ctctattgct ggtaccgggt taatcaccaa aacaggttcc ggcgatctgg
     1141 tacttaatgc agataacaat gactttactg gtgagatgca gattgaaaac ggtgaagtta
     1201 ccctgggccg cagcaactcc ctgatgaatg tcggcgatac gcattgccag gacgatccgc
     1261 aagactgcta cggtctgacg atagggagta ttgatcagta tcagaatcag gctgagctaa
     1321 acgttggctc gacccaacaa acttttgtgc acgcattgac gggctttcag aatggcactt
     1381 taaatatcga tgctggtggc aacgttactg ttaatcaggg cagttttgct ggcatcatcg
     1441 aaggtgctgg tcagctcacc attgcgcaaa acggcagcta cgtgctggca ggggcgcagt
     1501 cgatggcgct aaccggcgat atagtcgttg atgatggtgc ggtgctttcg ctggaaggcg
     1561 acgcggcaga tcttaccgct ctccaggacg atccgcagtc gatcgtgtta aacggcggtg
     1621 tgctcgatct ctctgatttc tccacctggc agagcggcac atcatacaac gatggccttg
     1681 aagtcagtgg cagcagcgga acggttatcg gcagtcagga tgtggtagat cttgcaggtg
     1741 gcgacaattt gcatatcggc ggcgacggga aagatggcgt ctacgtggtg gtcgatgcga
     1801 gcgacgggca ggtaagtctg gcaaacaata atagttattt gggcacaaca caaatcgcct
     1861 ccggtacgct gatggtgagc gacaactcgc agcttggaga tacccactat aaccgccagg
     1921 ttatctttac cgataagcaa caagaaagcg tgatggagat tacctccgac gttgacacgc
     1981 gttcagatgc ggcaggccac ggacgtgata ttgaaatgcg cgccgacggt gaagtggcag
     2041 ttgatgcggg ggtagacacg cagtggggcg cactgatggc tgacagcagc gggcagcatc
     2101 aggatgaggg tagcacattg actaaaacgg gggctggtac actggagctg accgccagcg
     2161 gtacaacgca gtcggcggta cgtgtcgaag aaggcaccct gaaaggtgat gttgctgata
     2221 tccttcctta tgcttcgtca ctgtgggttg gtgatggggc gacgttcgtt actggcgcgg
     2281 atcaggatat tcagtcaatt gatgctattt ccagcggcac tatcgacatc agcgatggta
     2341 cggttttgcg cctgaccggg caggatactt ccgtcgccct taatgcctca ctatttaacg
     2401 gcgatgggac gctggtgaat gccaccgatg gtgtgacgtt gacaggtgag cttaatacca
     2461 accttgaaac tgacagcctg acttatcttt ccaacgtgac ggttaatggc aatctgacca
     2521 atacgtccgg tgcggttagc ctgcaaaatg gcgtcgctgg cgatacgctg acggtaaacg
     2581 gtgattatac cggcggcggt acgctactgc tcgatagcga attaaacggc gatgactcgg
     2641 taagcgatca attggtgatg aacggtaata ctgctggcaa cacaactgtg gtggttaact
     2701 ccattacagg gattggtgag ccgacatcga caggcattaa aatggttgat ttcgcagctg
     2761 atcccacgca gtttcaaaac aatgcgcagt tcagtctggc aggcagcggc tacgtcaata
     2821 tgggagcgta tgactacacg ctggtggaag ataacaacga ctggtatctg cgatcgcaag
     2881 aagtaacgcc gccatcgcca cctgatccag acccgactcc cgatcctgat cccacgccgg
     2941 atcctgatcc aacacccgac ccggaaccta cgcctgctta ccagccggtg ttgaatgcca
     3001 aagttggcgg ttatctcaat aacctgcggg cggcaaatca ggcgtttatg atggagcgac
     3061 gcgatcacgc aggtggcgat ggtcagacgc tgaatttacg tgttatcggc ggagattatc
     3121 attacacagc agcggggcaa ctggctcaac atgaagacac ttctacggtg caacttagcg
     3181 gcgatctgtt tagcgggcgc tggggcacgg atggcgagtg gatgcttggg attgttggtg
     3241 gctacagcga taaccagggc gacagccgct cgaatatgac cggaactcgc gccgataacc
     3301 agaaccacgg ttatgccgtt gggctgacat caagctggtt tcagcacggt aatcagaagc
     3361 aaggggcctg gctggatagc tggctgcaat acgcgtggtt tagcaatgat gtttccgaac
     3421 aagaagatgg cacagatcat taccactcgt cggggattat cgcctcgctg gaggcggggt
     3481 atcagtggtt accggggcgt ggtgtggtga ttgaaccgca ggcgcaggtg atttatcagg
     3541 gcgtgcagca ggatgatttt accgccgcta accgtgcgcg cgtgtcacaa tcgcagggtg
     3601 atgatattca gacgcggctg ggtttacaca gcgaatggcg taccgctgtt catgtcatac
     3661 caacattaga tctgaattat tatcacgatc cccattcgac ggaaattgaa gaggatggca
     3721 gcactatcag tgacgatgcg gtgaagcaac ggggtgaaat aaaagtggga gtcacgggca
     3781 atatcagtca gcgagtgtcc ctgcgcggca gcgtggcgtg gcagaaaggg agtgatgatt
     3841 ttgcccagac ggcagggttt ttgtcgatga cggtgaaatg gtaatcactg tctagtcggc
     3901 acggttgccg gatgcggcgt aaacgcctta tccggcctac ggggagcatt tgtaggcctg
     3961 ataagacgcg gcaagcgtcg catcaggcat tggcgcggcc aaacatcaac ctcacttatt
     4021 ctgcgtgtgc agcatatagt tcacatccac gccggggccg agcttaaagg aattagtgat
     4081 cgggttgtaa tgcagcccag tgatatgccg ctcttttaaa ctggtctgat ccacccagcc
     4141 cagcaattct gccggtttaa taaacttctt cacatcatgc gtgcctttgg gcaccatgcg
     4201 caaaatatat tccgcaccaa ccaccgccat cagccatgac ttgccgttgc ggttaagtgt
     4261 cgagaaaaag acatcgccgc ctggtttcac cagttgcgca caggctctga ccactgactg
     4321 cggatcgggg acgtgctcca gcatctccat gcaggtcacc acatcatact gcccggcatg
     4381 ttttgccgcg tgcttttcca cggtttcctg cacataatcc acctgaatgc cgctttccag
     4441 cgcgtgcagt tttgccacct gcaatggctc aaagcccata tccagaccgg tcaccgtcgc
     4501 gccttcgcgc gccatactct cggccagaat gccgccgcca caaccgacat cgagcacctt
     4561 tttgccaaat aaaccgccag cacgctcggc aatatagccc agacgcagcg ggttaatgcg
     4621 gtgcaacggt ttgaactcac cttcaagatc ccaccagcgg gaggcgacgg cttcaaattt
     4681 agcgatctct tcgtggtcta cgttatggtt ttccggcgat ttttcggcat tcattggcac
     4741 ttctactccg taattggcaa gacaaacgag tatatcaggc attggatgtg aataaagcgt
     4801 ataggtttac ctcaaactgc gcggctgtgt tataatttgc gacctttgaa tccgggatac
     4861 agtagaggga tagcggttag atgagcgacc ttgcgagaga aattacaccg gtcaacattg
     4921 aggaagagct gaagagctcc tatctggatt atgcgatgtc ggtcattgtt ggccgtgcgc
     4981 tgccagatgt ccgagatggc ctgaagccgg tacaccgtcg cgtactttac gccatgaacg
     5041 tactaggcaa tgactggaac aaagcctata aaaaatctgc ccgtgtcgtt ggtgacgtaa
     5101 tcggtaaata ccatccccat ggtgacttgg cggtttataa cacgatcgtc cgtatggcgc
     5161 agccattctc gctgcgttac atgctggtag acggtcaggg taacttcggt tccatcgacg
     5221 gcgactctgc ggcggcaatg cgttatacgg aaatccgtct ggcgaaaatt gcccatgaac
     5281 tgatggccga tctcgaaaaa gagacggtcg atttcgttga taactatgac ggcacggaaa
     5341 aaattcccga cgtcatgcca accaaaattc ctaacctgct ggtgaacggt tcttccggta
     5401 tcgccgtagg tatggcaacc aacatcccgc cgcacaacct gacggaagtc atcaacggtt
     5461 gtctggcgta tatcgatgat gaagacatca gcattgaagg gctgatggaa cacatcccgg
     5521 ggccggactt cccgacggcg gcaatcatta acggtcgtcg cggtattgaa gaagcttacc
     5581 gtaccggtcg cggcaaggtg tatatccgcg ctcgcgcaga agtggaagtt gacgccaaaa
     5641 ccggtcgtga aaccattatc gtccacgaaa ttccgtatca ggtaaacaaa gcgcgcctga
     5701 tcgagaagat tgcggaactg gtaaaagaaa aacgcgtgga aggcatcagc gcgctgcgtg
     5761 acgagtctga caaagacggt atgcgcatcg tgattgaagt gaaacgcgat gcggtcggtg
     5821 aagttgtgct caacaacctc tactcccaga cccagttgca ggtttctttc ggtatcaaca
     5881 tggtggcatt gcaccatggt cagccgaaga tcatgaacct gaaagacatc atcgcggcgt
     5941 ttgttcgtca ccgccgtgaa gtggtgaccc gtcgtactat tttcgaactg cgtaaagctc
     6001 gcgatcgtgc tcatatcctt gaagcattag ccgtggcgct ggcgaacatc gacccgatca
     6061 tcgaactgat ccgtcatgcg ccgacgcctg cagaagcgaa aactgcgctg gttgctaatc
     6121 cgtggcagct gggcaacgtt gccgcgatgc tcgaacgtgc tggcgacgat gctgcgcgtc
     6181 cggaatggct ggagccagag ttcggcgtgc gtgatggtct gtactacctg accgaacagc
     6241 aagctcaggc gattctggat ctgcgtttgc agaaactgac cggccttgag cacgaaaaac
     6301 tgctcgacga atacaaagag ctgctggatc agatcgcgga actgttgcgt attcttggta
     6361 gcgccgatcg tttgatggaa gtgatccgtg aagagctgga gctggttcgt gaacagttcg
     6421 gtgacaaacg tcgtactgaa atcaccgcca acagcgcaga catcaacctg gaagatctga
     6481 tcactcagga agatgtggta gtgacgctct ctcaccaggg ctacgttaag tatcagccgc
     6541 tttctgaata cgaagcgcag cgtcgtggcg ggaaaggtaa atctgccgca cgtattaaag
     6601 aagaagactt tatcgaccga ctgctggtgg cgaacactca cgaccatatt ctgtgcttct
     6661 ccagccgtgg tcgcgtctat tcgatgaaag tttatcagtt gccggaagcc actcgtggcg
     6721 cgcgcggtcg tccgatcgtc aacctgctgc cgctggagca ggacgaacgt atcactgcga
     6781 tcctgccagt gaccgagttt gaagaaggcg tgaaagtctt catggcgacc gctaacggta
     6841 ctgtgaagaa aactgtcctc accgagttca accgtctgcg taccgccggt aaagtggcga
     6901 tcaaactggt tgacggcgat gagctgatcg gcgttgacct gaccagcggc gaagacgaag
     6961 taatgctgtt ctccgctgaa ggtaaagtgg tgcgctttaa agagtcttct gtccgtgcga
     7021 tgggctgcaa caccaccggt gttcgcggta ttcgcttagg tgaaggcgat aaagtcgtct
     7081 ctctgatcgt gcctcgtggc gatggcgcaa tcctcaccgc aacgcaaaac ggttacggta
     7141 aacgtaccgc agtggcggaa tacccaacca agtcgcgtgc gacgaaaggg gttatctcca
     7201 tcaaggttac cgaacgtaac ggtttagttg ttggcgcggt acaggtagat gactgcgacc
     7261 agatcatgat gatcaccgat gccggtacgc tggtacgtac tcgcgtttcg gaaatcagca
     7321 tcgtgggccg taacacccag ggcgtgatcc tcatccgtac tgcggaagat gaaaacgtag
     7381 tgggtctgca acgtgttgct gaaccggttg acgaggaaga tctggatacc atcgacggca
     7441 gtgccgcgga aggggacgat gaaatcgctc cggaagtgga cgttgacgac gagccagaag
     7501 aagaataatt ttacttcttc atgccaaaag ggagctatct cccttgtttg aattgaaaag
     7561 tccaggctgc aaagtctggg cttttgtcgt attagggcac ggtaaagttt ggctgtgccc
     7621 gtaaaaaatg gctggctata cacaaggaat gtggcaatga gtggtgaaaa aaaggcgaaa
     7681 ggctggcggt tctatggtct tgtaggtttt ggcgcaatag cactgctttc cgctggcgtc
     7741 tgggcgttgc aatatgctgg cagtgggcca gaaaaaacgt tgtcgccgct ggtggtgcac
     7801 aacaatctgc aaatcgatct caatgagccg gacctctttc tcgacagcga ctctctgagc
     7861 cagcttccca aagatctcct caccattccg tttctccacg atgttctgag cgaagatttc
     7921 gttttctatt atcagaatca tgccgatcgt ctgggcattg aaggcagcat tcgtcgtatt
     7981 gtctatgaac acgatctcac gctgaaagat aagctctttt cgtcactctt agatcagccc
     8041 gcgcaggcag cgctgtggca cgataaacaa ggccatcttt cacattatat ggtgctgatc
     8101 cagcgcagtg gtttaagcaa actgctggag ccattgttgt ttgccgctac cagcgacagc
     8161 cagttaagca aaacggaaat cagtagcatc aagataaata gtgaaactgt tcctgtttat
     8221 cagttgcgct ataacggcaa taacgccctg atgttcgcga cttatcagga caagatgctg
     8281 gtgttttcca gcacggatat gttgtttaaa gatgatcagc aggataccga agccacggcg
     8341 atcgcaggtg atttgttgag cggcaaaaaa cgctggcaag caagctttgg cctggaagag
     8401 cgtactgccg aaaaaacgcc agtacgccag cgcatcgtag tcagcgccag gtggctgggg
     8461 tttggctacc agcggttaat gccttctttt gctggcgtac gcttcgaaat gggtaacgac
     8521 ggctggcaca gttttgtggc gttaaatgat gaatccgcca gcgtagatgc cagtttcgat
     8581 tttacgccgg tatggaacag tatgcctgcc ggagccagct tctgtgtggc ggtgccgtat
     8641 tcacacggta ttgccgaaga gatgctttcg cacatcagcc aggaaaacga caagctgaat
     8701 ggggcgttag acggtgccgc ggggctgtgc tggtatgaag actcaaaatt gcaaaccccg
     8761 ctgtttgtcg gtcagtttga tggcactgcc gaacaggcgc aattgccagg gaaactgttt
     8821 acgcaaaata ttggtgcgca cgaaagcaaa gcgccagaag gtgttttgcc ggtaagccag
     8881 actcagcagg gcgaagcgca aatctggcgt cgcgaagtga gttcccgata cggccagtat
     8941 ccgaaagcgc aggcggcgca acccgatcaa ttaatgtcgg attatttttt ccgtgtgtcg
     9001 ctggcgatgc aaaacaaaac gctgcttttc tccctcgatg acacgctggt taataacgcg
     9061 ctgcaaacac tgaataaaac ccgcccggca atggtggatg taatacccac tgatggcatc
     9121 gttccgctct atatcaatcc acaaggaata gcgaaactgc tgcgtaacga aacgttgacc
     9181 agtctgccga agaatctcga accggttttt tataacgccg cacaaacttt attaatgccg
     9241 aagctggacg ctttatctca acaaccgcgt tatgtcatga agctggccca gatggaaccc
     9301 ggtgccgcct ggcagtggct acccataacc tggcagccgc tatgaggcac gggctgctgg
     9361 cgctgatttg ctggctgtgt tgtgttgttg cccatagcga aatgctgaat gtcgaacaat
     9421 ccgggctgtt tcgcgcctgg tttgtacgca ttgcacaaga acagctccgc caggggccaa
     9481 gtccacgctg gtatcagcag gattgtgcgg gcctggtgcg atttgcggcg aacgagacgc
     9541 tgaaagttca cgacagtaaa tggctaaaaa gtaacggttt atctagccag tatttgccgc
     9601 cagagatgac gctaacacct gaacagcgtc aactggcgca aaactggaat caggggaacg
     9661 ggaaaaccgg cccctatgtg accgcgatta atttgattca gtacaacagc cagtttattg
     9721 gccaggacat aaaccaggcg ctgcctggcg atatgatttt tttcgatcag ggcgatgccc
     9781 agcacttaat ggtctggatg gggcgttacg tcatctacca caccggaagc gccacgaaaa
     9841 ctgacaacgg aatgcgcgca gtcagtctgc aacaacttat gacatggaag gacacccgat
     9901 ggatacccaa cgattccaat cccaatttca ttggcattta tcgtttaaat tttctggcgc
     9961 gatagcagcg tgcttgtccc tgtctcttgt gggaacaggg cttgctaatg ctgatgattc
    10021 gcttccttcc agcaactatg cgccgcctgc cgggggaaca ttctttttgc ttgctgacag
    10081 cagttttagc agcagtgaag aggcgaaagt gcgactggaa gcgccggggc gtgattatcg
    10141 gcgctatcag atggaagagt acggcggcgt ggacgttcgc ctgtatcgta ttcctgaccc
    10201 gatggcattt ttgcgccagc agaaaaacct gcatcgcatt gtggtgcaac cgcaatatct
    10261 gggcgacggg ctgaacaata cgctaacttg gctgtgggat aactggtacg gcaaatctcg
    10321 ccgcgtgatg cagcgtactt tctcttctca gtcacggcag aatgtgactc aggcgttacc
    10381 cgaattacag ctcggcaatg ccattattaa accttcccgt tatgtacaga acaaccagtt
    10441 ttccccgctg aaaaaatatc ccctggtgga acagttccgt tatccactat ggcaggctaa
    10501 accgttcgag ccgcagcaag gggtaaaact ggaaggcgca tccagcaatt tcatctcgcc
    10561 gcagccgggt aacatttata ttcctctcgg ccaacaagag ccgggactgt acctcgtcga
    10621 ggcgatggtt ggtgggtatc gggcgacgac ggtggtgttt gtttccgata ccgtggcgct
    10681 tagcaaagtg tcaggtaacg agcttctggt atggaccgcg ggtaaaaaac agggtgaagc
    10741 gaagcccggc tcagagatct tgtggactga cggtcttggc gtgatgaccc gcggtgtgac
    10801 cgatgacagc ggtaccttgc agttacaaca tatatcgcca gaacgttcat acattctggg
    10861 taaggatgct gaaggcggcg tttttgtctc cgagaacttc ttctacgaaa gcgaaatcta
    10921 caacacccgc ttgtatattt ttaccgatcg cccgctatat cgcgcaggcg atcgtgtcga
    10981 tgttaaagtg atgggccgcg agttccacga tccgttgcat tcatccccca tcgtcagcgc
    11041 cccggcgaag ctttcggtgc tggacgctaa cggcagtctg ttgcaaaccg tcgatgtcac
    11101 gctggatgcg cgcaatggcg ggcagggaag tttccgcctg ccagaaaatg ccgtagccgg
    11161 aggttatgag ttacgtcttg cttaccgcaa tcaggtctat agcagcagtt ttcgcgtggc
    11221 aaactacatc aagccacatt tcgagattgg tttagctctc gccaaaaaag agttcaaaac
    11281 tggcgaagcg gtcagcggca aactgcaact tctctacccg gatggcgagc cggtaaaaaa
    11341 tgcccgcgtg cagttaagtt tgcgcgctca gcaattatca atggtcggta acgatttgcg
    11401 ttatgccgga cgtttccccg tgtcgctgga aggcagcgaa acggtgtccg acgccagcgg
    11461 tcatgtggcg ttaaatctcc ccgccgccga taaaccgagc cgctatttgt taaccgtctc
    11521 cgccagtgac ggcgcggcgt atcgcgtcac caccaccaaa gagatcctca ttgaacgcgg
    11581 tctggcgcat tactcattaa gtactgccgc acaatacagt aatagcggcg agtcggttgt
    11641 gttccgttat gccgcgctgg aatcttcaaa acaggttcct gttacgtatg aatggttgcg
    11701 tctcgaagac cgcacgagcc atagcggaga gctaccgtca ggcggcaaat cctttaccgt
    11761 caatttcgct aaacctggca actacaatct gacattacgc gataaagacg gcttaattct
    11821 cgctgggtta agtcatgccg tcagcggtaa gggcagcacg gcgcatactg gtacggtaga
    11881 tatcgtggcg gataaaacgc tgtaccagcc aggcgaaacc gcgaagatgc tgattacctt
    11941 tccggagcca attgatgaag cattattgac gctggaacgc gatcgcgtgg aacagcagtc
    12001 gctgctttcg catccggcaa actggctaac gctacaacgt ttaaacgata cccagtatga
    12061 agcccgggtt ccagtgagca attcctttgc gcctaacatc actttttcgg tgctgtatac
    12121 ccgtaacggt cagtacagtt ttcagaacgc cgggatcaaa gttgccgttc cccagctgga
    12181 tatccgggtg aaaacggaca aaacccatta ccagcctggt gaactggtca atgtcgaatt
    12241 aacctcgtcg ctgaaaggta aacctgtttc tgcgcagcta acggtaggcg tggtcgatga
    12301 aatgatctac gcgctgcaac cagaaatcgc tccgaatatc ggcaaatttt tctatccgct
    12361 ggggcgtaac aatgtgcgta ccagctccag tctgtcgttt atcagctacg accaggcgct
    12421 ctccagcgag ccggttgcgc ctggcgcaac taaccgcagc gagcggcgag taaaaatgct
    12481 tgaacgtcca cggcgtgaag aggtggatac cgcggcatgg atgccgtcac tcacaaccga
    12541 taaacaaggc aaagcgtatt tcacgttcct gatgcctgat tcgttaaccc gctggcgtat
    12601 caccgcgcgt gggatgaacg gcgacgggct ggtcgggcag gggcgtgctt atctgcgttc
    12661 ggaaaaaaat ctctacatga agtggagtat gccaacggtg tatcgcgtgg gcgacaaacc
    12721 ggcggcagga ctgtttatct tcagtcagca ggataacgaa ccggtggcgc tggtgactaa
    12781 atttgcaggc gctgagatgc gccagacgct gacgctgcac aaaggggcga attatatttc
    12841 gctgacgcag aatattcagc aatctggctt gttaagtgca gaactgcaac aaaatgggca
    12901 agtgcaggac agcattagca caaaactgtc ttttgtggat aacagctggc ccgttgaaca
    12961 gcagaaaaat gtcatgctcg gtggtggcga taacgcgctg atgttgcccg agcaggcgag
    13021 caatatccgg ctacaaagta gtgaaacgcc gcaggagatt ttccgcaaca atcttgatgc
    13081 gttagtcgat gaaccgtggg gtggcgtaat caacaccggt agccgtctga tcccgctcag
    13141 tctcgcctgg cgttcgcttg ccgatcatca aagtgccgcc gctaacgaca ttcgtcagat
    13201 gattcaggtt aaccgtctgc ggctgatgca actggcgggg cccggagcgc gctttacttg
    13261 gtggggtgaa gatggcaatg gtgacgcctt ccttacggca tgggcatggt acgccgactg
    13321 gcaggccagc caggcgatcg gcgtaacgca acaaccggaa tactggcagc atatgctcga
    13381 cagctacgcg gagcaggcag ataacatgcc gttattgcat cgggcgctgg tgctggcatg
    13441 ggcgcaggag atgaatttgc cgtgcaaaac gttgttgaaa gggttggatg aagctatcgc
    13501 ccggcgcgga actaaaactg aagatttctc tgaggaagac acccgcgata tcaatgatag
    13561 cctgatcctc gatacaccgg agtctccact ggcagatgcg gtggcaaacg tcttaaccat
    13621 gacgttgctg aaaaaagcgc agttgaagtc cacggtgatg ccacaggttc agcaatatgc
    13681 gtgggataaa gcggcaaaca gcaatcagcc gctggcgcac acggttgtgc tgcttaatag
    13741 cggtggcgac gctacccagg cggccgctat tttaagtggt ttgaccgctg agcaatccac
    13801 tattgaacgc gcgctggcca tgaactggct ggcgaaatat atggcgacaa tgcctccggt
    13861 tgtgttgcct gcgcctgcgg gcgcatgggc caaacataag ttaactggag ggggcgaata
    13921 ctggcgttgg gttggtcagg gtgtgccgga cattctctct tttggtgacg aattatcgcc
    13981 gcaaaatgtg caggtccgct ggcgtgaacc ggcaaaaacg gctcaacaaa gtaacattcc
    14041 ggtaaccgtt gaacgccagt tgtatcggct tatcactggt gaagaagaga tgagctttac
    14101 tctgcaaccg gtgaccagca atgagattga cagcgatgcg ctgtatctcg atgaaatcac
    14161 gcttaccagc gagcaggatg cagttctgcg ctacggtcag gtggaagtac cgctcccgcc
    14221 gggagccgac gttgagcgca caacatgggg catttcggtc aataaaccca acgccgcgaa
    14281 acagcagggg caattgctgg aaatagcgcg aaatgaaatg ggcgaactgg cctatatggt
    14341 gccggtgaaa gaactgacgg gaacggtcac tttccgccat ttgctgcgct tctcgcaaaa
    14401 agggcaattc gttctgcctc ctgctcgtta tatgcgttcc tatgcacctg cacagcaaag
    14461 tgttgcggca gggagcgaat ggaccaggat gcaggtgaaa taagtgaact ggcgcagaat
    14521 cgtctggcta ttggcgttgg ttactctgcc aacgcttgcg gaggagccgc cgttacaact
    14581 ggcgctgcgg ggcgcgcagc atgaccaact gtataagtta tcgtcatcag gcgtgacaaa
    14641 tgtctcgacg ttacctgaca cgctcaccac accgttaggg agcttatgga aactgtatgt
    14701 ttacgcctgg ctggaggata cccatcaacc agaacaacca taccagtgtc gcggcaactc
    14761 cccggaagag gtttattgtt gtcaggctgg ggagagcata acgcgcgata ccgcgctggt
    14821 acgttcctgt gggctctatt ttgcgccgca gcggttgcat attggcgcag atgtgtgggg
    14881 gcaatactgg caacagcgtc aggcacctgc gtggttagcg tctcttacta cgctgaagcc
    14941 agaaacctcg gtaacggtta aatcgctgct tgattcatta gcgacgttac ctgcgcaaaa
    15001 caaagctcag gaagtcttac tggatgtggt gctggacgag gcgaaaatcg gtgtcgcatc
    15061 catgctcggt agtcgggttc gggtgaaaac ctggagctgg tttgccgatg ataaacagga
    15121 aatccgtcag ggtggttttg ctggctggtt aaccgatggt acaccgctgt gggttactgg
    15181 cagtggcacc agcaaaacgg tactaacccg ttatgcgacg gtgcttaatc gggttttgcc
    15241 agtgccgacg caggttgcca gtgggcaatg tgtagaggtg gagctgtttg cccgctatcc
    15301 gctgaagaaa atcacagcgg aaaagagtac gacggcagta aaacccggcg tacttaatgg
    15361 gcgctatcgt gtgacgttta ctaatggtaa ccacattacg tttgtcagcc acggtgagac
    15421 gaccttactg agcgaaaagg gcaaactgaa attgcagtca catctcgatc gtgaagagta
    15481 cgttgccaga gttctggatc gcgaggcgaa aagtacgccc cctgaggcag cgaaagccat
    15541 gaccgtggcg attcgcactt ttttacagca aaacgccaat cgggaaggcg attgcctgac
    15601 catccctgac agcagcgcca cgcagcgtgt ttcggcttcg ccagctacga cgggggcgcg
    15661 aacgatggcc gcctggacgc aggatctcat ctatgcgggc gatccggttc attatcatgg
    15721 cagtcgcgct accgaaggga cgctttcctg gcggcaagcc acggcccagg cggggcaggg
    15781 agagcgttac gaccagatac tcgcgtttgc ttatcccgac aacagcctta gccgctgggg
    15841 agcgccacgc tcaacctgcc agttattacc caaagcgaaa gcctggctgg cgaaaaaaat
    15901 gccgcagtgg cggcgtatat tacaagctga gacggggtac aacgaaccag acgtgtttgc
    15961 ggtttgccgt ttagtctcag gtttccccta taccgatcgg cagcagaaac ggctgtttat
    16021 tcgtaacttc tttacgcttc aggatcggct ggatttaacc catgagtatc tgcacctggc
    16081 ctttgatggt tatcccaccg ggcttgatga gaactatatc gaaacgctga cccgacaatt
    16141 attaatggac taatgctatg cgaaagattt ttcttccgtt gttactggtg gcactttcgc
    16201 ctgtcgctca cagtgaaggt gtgcaggaag tcgagattga cgcaccgctc tctggctggc
    16261 atccggtaga gggcgaagat gccagttttt cacaatcgat caattacccg gcgtcgtccg
    16321 tcaacatggc agatgatcaa aatatctcag cgcagatccg cggcaaaata aagaattatg
    16381 ctgcggcggg taaagttcag cagggccggc tggtggtcaa cggtgccagt atgccgcaga
    16441 gaattgaatc cgatggttca tttgcacgcc cttatatttt cactgaaggc agcaacagcg
    16501 tgcaggtcat cagcccggat gggcaaagcc gacaaaaaat gcagttttac tcaacgccgg
    16561 gtacaggaac gattcgtgca cgtttacggc tggttctctc gtgggatacg gacaataccg
    16621 atctcgatct ccatgttgtc acgcccgatg gcgaacacgc ctggtacggt aacacggtgc
    16681 tgaaaaacag tggtgcactg gatatggatg tcacgacggg gtacggaccc gagattttcg
    16741 ccatgccagc gccagttcac ggacgctatc aggtgtatat caactactat ggcggacgca
    16801 gcgaaacgga attaaccacc gcccaactga cgctgatcac cgatgaagga tcggtcaatg
    16861 agaaacagga aacatttatt gtaccgatgc gtaatgctgg cgagctgacg ctggtgaaaa
    16921 gttttgactg gtgattaatg gggttacctt cgctgtcgat gttcaagaaa acgcccgata
    16981 actttcgcta tcgggcgttt ttgttcgtta attcaaccgt tcaatcacca tcgcaattcc
    17041 ctggccgcca ccaatgcaca gtgttgccag ccccagcgtt ttatcgcgtg cctgcatggc
    17101 atgtaatagt gtgaccagaa tacgagcacc actggcaccg ataggatgcc cgagcgcgat
    17161 ggccccgccg ttgacattca ctttctcagg atcaaagccc aaggttttcc caacggcaag
    17221 gaactgtgca gcaaatgctt cattagcttc gatgagatca atgtctgcca gttgcagccc
    17281 cgccagttgt aacgcttttt gcgtggcagg tactggcccc atacccatca atgcgggggg
    17341 cacgccaccg ctggcataac ttttaatgcg agccaggggg gtaaggcctg ctgccagcgc
    17401 cgcagattct tccataatca ccagagcggc agcaccgtcg ttaataccag acgcgttccc
    17461 agcggtgact gttcctgctt tatcgaaggc cgggcgcaat gcacctaacg cttcagccgt
    17521 tgaattcgct ttcgggaatt cgtcttgact gaagacgaag gttttctttc gagtgacaac
    17581 atttaccggg acgatttcgg ctgtaaaagc accggactca attgcggctg ccgctttacg
    17641 ctgtgaatgt agcgccagtt catcctgcat ttcacgggta attccgtact ctttagccac
    17701 gttttcggcg gtaatcccca tatgataacc atgggtggcg cacatcaggc catcgcgcag
    17761 gattacgtca taaacctgtc cgtctccaag acgataacca gagcgtgctt ttgcatcgag
    17821 taagtagggg gctaaactca tattttccat accccccgcc acaatgctct gcgcctgacc
    17881 tgcctgaatg gcctgggcgg caagcgccac acttttaaga cccgaaccac atactttatt
    17941 gaccgtgaat ccgcacaccg tttctgccag cccgcttttt aacagtgcct gacgcgccgg
    18001 attttgcccc agcccggctt gtaacacgtt acccataatc acttcatcaa cgtgttgtga
    18061 atcgattttt gcacgttcaa tggcggcttt aattactgtc gcccccaggt cgatggcgct
    18121 ggtggaagcg agtgaaccgt taaaactacc gatagcagta cgtaccgcac tgacgatgac
    18181 acaatttttc attttatatt ccttctgttt gtaggggtgc cgtcagaaca gcgttaaacc
    18241 aatgacgaaa atgacaccgg agaagagcag ggcagtgatg cagtagccca tgatgtcgcg
    18301 gacaccgagt ccggcgattg ccagtgctgg cagcgcccag aatggttgtg ccatgttcat
    18361 ccattgctcg ccgtaggcga tcgccattac cgatttaccg agatcagcgc ccagcgcctg
    18421 ggctgcgggt atcacgaaag gtccctgaat aacccagtga ccgccgccag acggaacggc
    18481 gaagttaatc agtgcagaac taaaaaaggt cattaccggg aaggtgtctt tgttcgcaac
    18541 attgatgaag aattcggtaa tgagtccgcc cagaccggaa tgctccatca tcagttggat
    18601 cccagcgtag aaggggaatt gcaccagaat accggcagta ctgcgtgctg ccgcgctgat
    18661 agcacgcata taagccattg gcgttttatg tagcagcaga cccgcaatca taaacatcag
    18721 gttgacggta ttgatggtga tgttaaagcc atgttcgctg aagtacatcg caaggtaagc
    18781 gataccgagt gcgccgatga tcaacgtcag aatgcggctt tcttccagtc gctcggatgg
    18841 tggggcatct ttcggtagct gcttttgaaa atcagcctct tccatgagta gtttcggatc
    18901 gatactcacc acgtcagacg gttttggcat catcatgcgg gtgataaatg gcatcaccac
    18961 aatcaacgcc acagtgatga aaatgttaaa accactgaac agagtatcgc ccaccgggat
    19021 cagcccggcg atatgctcaa ccgggttgcc cggtgttgca gccaacagag gcattgagcc
    19081 agagaagccg ccaccccagg tgagaaaacc aatgtaggcg caggcaatga gcaacggata
    19141 atcagaaccg ggtactcggc gggcgacttc acgggcaaac attgcgccga caaccaaacc
    19201 aaatccccag ttgatgacac aagcgactga accgaagaaa gtgaccagca tgacgccctg
    19261 tacgggcgtc tttgcggcgg aggcggcagt acgcagcaaa cttttcaccg gagcagagct
    19321 ggcaagggca tgaccggtaa cgatgataag cgccatctgc ataccaaacg ccagcaagtt
    19381 ccagaaaccg tcaccccaca ttttcaccat gctgatcggc gtttgtggtg ttaaccaaag
    19441 cgcgatcacg aatgttagca atgtcagcaa catggcaaag atcagtggat cgggaagcca
    19501 ccggctgaca aaacgcgtca taaaacgcga tatgcgacca atcataaatc accccgttgc
    19561 gtattcagat cggcggcgac ttcaaaccga gcttctgttt tggcacgcac ggtggctaaa
    19621 tcacacccgt cggcaatttc ggtgagccac attttgccgt caataaaacg aaagacagcc
    19681 agttcagtaa ccagcatatg caccgcatgt tgcgcagtga gtggcatggt gcagcggcgc
    19741 aaaatttttg ctgaaccatc tttggcgcaa tgttccatgg cgatgatcac tttgcgcgac
    19801 ccggtcacca gatccatcgc gccacccata ccgggcacca ttttcccagg cactacccag
    19861 ttcgcgaggt ttgcttcttc gtctgcttgc aaaccgccga gcacgcaggc atcaatatga
    19921 ccgccacgga ttagcgcaaa tgacatggcg ctatcaaaca tggctgcacc gggtaaaaca
    19981 ccgcacggtt gcccgccagc gttcaccaga tctggatgcg ctgtcgtgac cgggcctaaa
    20041 ccgaggaagc cgttttccga ttgcagagtg atatgaatac cctccggtaa ataattggcg
    20101 accattgtgg gtaaaccgat ccctaagtta acgatgtcac catcacgaag ctcttgcgcc
    20161 acacggcgcg caatacgttg tttcgcatcc attatttgct ctcctgtgaa acgatgatgt
    20221 ggtcgataac ggcaccaggg gtgacaatat ggtcaggttg cagctcgccg gtttcgacca
    20281 gttcatctgg ctctaccagc gtgatatcag ccgcaagggc tatcaggggg ttaaagttgc
    20341 gggcgctaag ttgataggtc aggttgccaa gtgtgtcgca acgatgagcg cgaattagcg
    20401 ccaggtcggc gcgcagtggg cgttcgagca gccaggtttt accgtcgagt gtcagtgtct
    20461 gtttgccttc ctctacgacg gtgccgacac ccgttggggt gagaaaacca ccaagtccag
    20521 ctccaccaca gcgaatttgc tcgattagcg taccttgcgg caccagaacg acgtccatct
    20581 caccagatat catgcgccga cctgtttccg ggttggtgcc gatatgtgaa gcaatcactt
    20641 tgcggactcg accattgacg atgagcggac cgatgccggt atcaacaaac gcggtatcat
    20701 tggctatcaa tgtcaggtcg cgaacaccag attccagtaa tgcttcaacc aggcgggatg
    20761 gagtgccaat ccccataaat ccgcccacca tgatggtcat gccgtcacga aagaagccgg
    20821 tggcgtcttg taatgtcatc aattttgttt tcatttttta tccctcttgc atacaccgtt
    20881 gtgggtacag tcaggcaata gcaaggggag tgccagaatg agattattgg atttagttat
    20941 ttaactaatt gaatatgaag ggataaattg gatggggatt tataaggaga atgagcggct
    21001 atgcagaaaa ttgcgcactg tacaaatttc tgcatagcaa gttttggtgt tatacatccg
    21061 ccggatcgat accgtattcc tggagtttat acatcaatgc acggcgactg atgcccagca
    21121 ttaaagcagt gcgggttcgg tttccttctt gttgttccag cacttccata atgatgcgtt
    21181 tttcgacgcg tttaatttcc tcttttaaat tacgctcacc gacaggggct gtttttacct
    21241 cgccagcatt acagactggc tgacgaatct gtggcggaag atcctcagaa aaaatgatcg
    21301 ggcctgaatt catcacgacg gcgcgttcaa taacgttgga aagctctcga atatttcccg
    21361 gccatgacca ggcggtaagc agtgacattg ccatcggatc gatgtcgata atatcgcgct
    21421 gattctcact actgaatttt tgcaaaaagt gattagctaa cagggaaata tcttcccggc
    21481 gatcgcgcag aggcggcagt attaaatgaa taacgttaag gcgataaaag agatcttcac
    21541 ggaaggtgcc ttcttttacc attgcctgca agtcacggtt ggtggcacca atgatgcgga
    21601 tatcaacttt tatggtctga tggccgccaa tccgttcaaa ttcccgttcc tgtagaatgc
    21661 gtagtaattt ggcttgtagt accagtggca tttcgccaat ttcgtcgagg agcagagtac
    21721 cttcgttggc tcgttcaaat aatccctgac gcaaggtttg tgcaccagta aatgcacctt
    21781 tttcatgacc aaacagttca ctttcgagca acgattccgg cagcgccgcg cagttgactt
    21841 taatgaacgg cccctttgcc cgccgcgaat tgtagtgaat cgctctggca atcaactctt
    21901 tcccggtgcc gctttcaccg ctaatcaaga cgctggcctg agaaagggca attttggcgg
    21961 tgtctttgca gatgtccatc atcgccgggc tgttggtgag aatgtgcccc cattgccagc
    22021 tggtgctcag tgcctggtgc agatgactga tctctttttt cattgactgg agttgtaaag
    22081 cgcgctgaac gattaaattc aactcatcga gatcaaacgg tttaataaca tagtcgaagg
    22141 ctccgcagcg tagcgcttcg acggcggttt ccacttccgc ataggccgtc atcagaataa
    22201 cgggtgtccg ggtctcatgg ctgcgcatct cctttagtgc cttgatgccg tccatctctg
    22261 gcatgcggat atccatcaac accacatcag ggtgaatatc ggcaaacagg tgtaatgctg
    22321 tgcgtccgtt gttcgcacaa tttgtttcga atccttgtag tgcaaaagcg gtgctcagca
    22381 tacggcgaac attatcttca tcatccacaa taaggatgcg attaatagca gtcatacagt
    22441 ctgatttccc tgcgggttga tcggtaaaat aagcgtgaag gttgcgccgt agcccggcaa
    22501 actggcgacg cgaatatcac cctgatgggc attaatgatg cgttgactta acgccagacc
    22561 aagcccggtt cctgaggctt tggtggtgaa aaagggatcg aagatctttt tttgcagcga
    22621 gagatcaatg ccacagccgt tgtcctctat cgaaatggcc tgttgtgagt cgctgtattg
    22681 ccaggtttga atgcgaattt tccctcgtgc gctgatagcc tggacggcat tgatcaggat
    22741 attcagtagt acctgtttga gcagttcacg atcggcgtta atcgggctta attcattatc
    22801 cagttcgctt atgaagtcga cccgcgcttg tacgccggcg gtttgtacca gtaccagagt
    22861 ttcttcaacc aatgcattga ggctgacttg ttgccattga ctgtggcgtg gacgtgaaaa
    22921 ttcgagcaat tgctgaataa ctttgttaat tgaatcgatt tctttgagta ctacggacag
    22981 atattcctga tgtattgggt cactggtttg ttggcgcaag atctgtacat aaccacgaat
    23041 agccgttaac ggattacgta cttcatgcgc gacgccagcc atcagctcac ccagtgtggc
    23101 gaggcgttct gcttgcgcca tgcggcgctg ggtttctttg cgggcagtta aatcagagaa
    23161 aatcaccaaa gcacctatca tttcaccgtg cgtgttatga atacgactgg tagtgacact
    23221 gagttcaatg gtgcggtcac gacctggaaa actgatctcc agcgccacat gttcggtgcc
    23281 atgttccagc gtatccagta ctggactgta gaactgagta ttgtcgaaca acatggagta
    23341 aggctgccct accagttcat ggcgttgata gccagtgata acttctgctg ctgggttcat
    23401 ggtggttaca tcaccctggc ggtcaatggc aatgacgcca tcggcagcgt tttcaataat
    23461 cagatcgtta agtgtccgcg tttcacgcag tgcctgggcg aggttattaa cactctgact
    23521 gatttgcccc atttcaccgg gcaattgtgg taatcgagtg ggaatatttt gtgccagagt
    23581 cgagaggcca tcggtgatga tatcaatatt ggcgctcagg cgacgggaga aaaggacaat
    23641 caatagcagg cttatcagca aaccggcggt gagcacaatg ataatcctca catccatttt
    23701 ccaggcctgg cggcgaatat cttcggttaa ttcattggcc cagatatagc cgaggatttc
    23761 accattacgc tcaatgggga gcattgaatt caaaatatcg ccgcgcacct gcctgcctga
    23821 ataaaccaaa ggggtatttg tacgcatgac ttcgcgacca gggtgatctg cggcaatggt
    23881 aacgccgaca ttattctgat atagcgctga aggcgcgtag gtgattatcg catccagcat
    23941 tttgttgtaa taaccagcac cgatgccagg gaaggcgtga gtgatatttt cggtaatggg
    24001 ggcaagttct gcatttaatg cgcggatacg ctcctcacgt ggtaagtcga tgtagagatc
    24061 atagcgatcg cctagtgcct gattaagcag gttgaccacg gcagaaagtt ttttctcttt
    24121 ttcagataag actgctgaac gtccttccgt ttctacgata taaccaatag taagcgttgg
    24181 gacaatgacc atcaggattg ccatcaggat catttgattg cgtaagcggc gtggataaat
    24241 ccacttcata taatgcatgc tgaaatccac tagtcttgtc cggtatatga cgattatcag
    24301 aggttaaggt gatgatttct cggcggtgta tcatattcca gagaagagag aacattgcgg
    24361 taacacgctt ttaccgctac cttaaccaca ctccatcggt cacctgaggc ggagcttcgc
    24421 ccctttgaaa taccttgctt cttttcgtac aaccctgaaa gcctcgcgct acatgttcag
    24481 agcattggcg ttagtgctct ggctgttgat tgctttttca tccgtttttt acatcgttaa
    24541 tgcgttacat cagcgagaat cggaaattca tcaggaattt aatctgagtt ccgatcaggc
    24601 tcagcgcttt attcaacgca cctctgatgt gatgaaagag ctgaagtaca tcgccgaaaa
    24661 tcgcttatcg gcagaaaacg gtgtgctttc cccgcgtgga cgagaaacgc aggcggatgt
    24721 gcctgcgttt gaaccgctgt ttgccgactc cgattgttcc gcaatgagta acacctggcg
    24781 aggttctctg gagtcattgg cgtggtttat gcgctactgg cgcgataatt tttctgcggc
    24841 ttacgatcta aaccgggtat ttttaatcgg cagcgataac ctctgcatgg ccaatttcgg
    24901 tctgcgtgat atgccagtgg aacgcgatac cgcgttgaaa gctttgcatg aacgcatcaa
    24961 taaatatcga aatgcaccac aagatgatag cggcagtaac ctctactgga tcagcgaagg
    25021 tccgcgccct ggcgtcgggt atttttacgc gttgacgcca gtttatctgg cgaaccggtt
    25081 gcaggcgctt ttgggtgtcg agcagaccat ccggatggag aactttttct taccgggtac
    25141 gttgccgatg ggggttacca ttcttgatga aaatggtcat accctgattt cgcttaccgg
    25201 accagaaagt aaaattaagg gcgatcctcg ctggatgcag gaacgctcct ggtttggcta
    25261 tacggaaggg ttccgggagc tggtgctgaa gaaaaatctg ccaccctcat cgctaagcat
    25321 cgtgtattcg gtgccggttg ataaggtgct ggaacgcatt cgcatgttga tccttaacgc
    25381 aattttgctg aatgtgcttg ccggagctgc attgtttact ctcgcacgga tgtacgagcg
    25441 acgtattttc attccggcgg aaagcgacgc cctgcgactg gaagaacatg agcagttcaa
    25501 tcgcaagatt gtcgcctccg cgccagtggg tatctgcatt ttgcgtaccg ctgatggcgt
    25561 caatatttta agtaacgaac tggcgcatac ctatctcaat atgcttacgc atgaggaccg
    25621 ccaacgactg acgcaaatta tctgtgggca gcaggtcaat tttgttgatg tcctgaccag
    25681 caacaatacc aatctgcaaa tcagcttcgt ccattcgcgc tatcgtaatg aaaacgtggc
    25741 catttgtgtg ctggtggatg tttcttcgcg cgtgaagatg gaagagtcgt tgcaggagat
    25801 ggcacaagca gcggaacagg cgagccagtc aaaatcgatg ttccttgcca ccgtcagtca
    25861 tgagctgcga acgccgctgt atggcattat cggtaacctg gatctgttgc aaaccaaaga
    25921 gttaccgaaa ggcgtcgatc ggctggtgac ggcaatgaac aactcttcca gcctgttgtt
    25981 gaaaattatc agcgatattc tcgatttctc gaagattgaa tcggaacagt tgaagatcga
    26041 accgcgtgag ttttcaccgc gtgaagtgat gaaccacatc accgccaact atttaccgct
    26101 ggtggtacgc aagcagttag gcttgtactg ctttattgaa ccggatgtgc cagtggcctt
    26161 aaatggcgac ccgatgcgtt tacagcaggt catctccaac ctgttgagta acgccataaa
    26221 attcaccgat accggctgta tagttttgca tgtccgcgct gatggcgatt atctctctat
    26281 ccgtgttcgc gataccggcg tggggattcc ggcgaaagaa gtggtgcgct tgtttgatcc
    26341 cttcttccag gtcggaacgg gcgtacagcg aaacttccag gggaccggtc tgggtctggc
    26401 gatttgtgaa aaactgatca gcatgatgga cggcgatatc tcggtagatt cagaaccggg
    26461 aatgggcagc cagtttaccg tgcgtattcc gttgtacggc gctcagtacc cgcagaaaaa
    26521 aggcgtggaa gggttgagtg gtaaacgctg ctggctgtcg gtccgcaatg cgtcgctctg
    26581 tcagttcctg gaaaccagtt tgcagcgcag cggcatcgtc gttacaacat acgaagggca
    26641 ggaaccgact cccgaagatg tgttaattac tgacgaggta gtgagtaaaa aatggcaggg
    26701 tagagcggta gtgaccttct gtcgtcgcca tattggtatt ccgctggaga aagcgccagg
    26761 ggagtgggta cacagtgtgg ctgccccgca tgagctaccg gcattgttgg cgcgtattta
    26821 tttgatcgag atggagagcg acgatcctgc taacgctctg ccgtcgacgg acaaagcggt
    26881 cagcgataat gacgatatga tgattctggt cgtggatgat catccgatta accgacgtct
    26941 gctggcagat cagttgggat cgttgggcta tcaatgtaaa accgcgaatg atggtgtcga
    27001 tgcgcttaat gtacttagca agaatcatat tgatatcgtg cttagcgacg tcaacatgcc
    27061 aaatatggat ggttaccgct tgacgcaacg cattcgtcag ttgggactga cgttgccggt
    27121 aatcggagta actgctaatg cgttggctga agagaagcag cggtgtctgg agtccggtat
    27181 ggacagctgc ctgtcgaagc cggtaacgct ggatgtgata aaacagacgc tgacggtata
    27241 tgccgagagg gtcaggaaat cgcgggaatc gtaggacgga taaggtgttt atgccgcatc
    27301 tggcattcag tgcaaatgcc agatgcgacg ctgacgcgtc ttatctggcc tactttaatg
    27361 taatatgttg aaataatggg aatcgtaggc cgcataaggc gtttacgctg catccggcaa
    27421 tcggtgcaaa tgccagataa gacactaacg cgtcttatct ggcctacaga tgattagtct
    27481 ttatctgccg gacttaaggt cacagaagag agataattca gcagggcgat atcgttctcg
    27541 acacccagct tcatcatcgc agatttcttc tggctactga tggttttaat actgcggttc
    27601 agctttttag cgatctcggt caccaggaag ccttccgcaa acaggcgcag aacttcactc
    27661 tcttttggcg agagacgctt gtcaccgtaa ccaccagcac tgattttttc caacaggcga
    27721 gaaacgcttt ccggggtaaa tttcttccct ttctgcagcg cggcgagagc tttcggcaga
    27781 tcggtcggtg caccttgttt cagcacgatc ccttcgatat ccagatccaa taccgcacta
    27841 agaatcgccg ggttgttgtt catagtcaga acaatgatcg acaggcttgg gaaatggcgc
    27901 ttgatgtact tgattaaggt aatgccatcg ccgtacttat cgccaggcat ggagagatcg
    27961 gtaatcaaca catgcgcatc cagtttcggc aggttgttga tcagtgctgt agagtcttca
    28021 aattcgccga caacattcac ccactcaatt tgctcaagtg atttgcgaat accgaacaag
    28081 actatcggat ggtcatcggc aataattacg ttcatattgt tcatgtaata ggctaccttg
    28141 ctacagcaag ctcttgacat aactgtcaat gtcgctgatg tatttttcta ttcctggaac
    28201 atccttctca cgaatcagat gttccagcgt ttcacataac tgcttgccgg gtaccagatt
    28261 tagcatggca aatacgcctt taagacgatg agccgtttgg gctaacgcag caaagtcact
    28321 ggttgctgct tcagtataca gcctcttaac atcatccggt actgtgtcta caaagagcgc
    28381 ataatagccg ctggcatgga gttgcgcatt ttcatctccg cccagaggcg attctgtcac
    28441 ctcttcctgc gccagttgca cttcaattaa ttgtaagacc gcttcctgca tagcgttgct
    28501 catattgaag ttgacgcaca attgaccagg cccaatttcc cgtacgccag actcatcatc
    28561 gcttaaaagc aagccagagg cagtaagatt agacggatta tccgttaaaa agatatcata
    28621 atcttgacta attaatcttt catcgggtgt gatacaggtt gcaccccaat tttctaactg
    28681 gcgagtgaca atattccgaa tttctgccga agtaacatcc accattacgc agacatcatc
    28741 cagtaaacgc tcttcttcct cttcaacttc cgggtcagcg gcgagcattt tgatatgcac
    28801 agagtagcgt gtaccaagcc catcccgcgt tttgatgttt aaatgaccgc ccagtttacg
    28861 tgccagttga tcgctcagcc agaatgccag cgggtccgcc tttccataac gatcgttttg
    28921 ggtctggttg ataaacggga agtgcaaatt atccatttca tgaatactta cgccttctcc
    28981 cgtgtccaga atgcgaaacg tcaggcggtc ttcggaggac tcatcctgat caacctcaag
    29041 ggtgattttt cccaattgcg ttgaggtcac ggcatattgc atcagtagca gcaaaatacg
    29101 tcgtaaggca tcgcgatcgc cacggcgcat atcgtgtgct ttcagatgat tgttaatcag
    29161 cagttgcaga cctttacgct tgatggcagg caacactgaa ggcacaactt catcaattaa
    29221 atcctgcacg gagaacagca ccgtctcact tttccagcta tcgtccgcaa gcatgttcgc
    29281 taactgtatt tcatcgacca gccgcaccag cacatctgcc tgattcgcca gttgtttgct
    29341 ttccggggcg ttgagtttag ccgcgctctc cgccagggac tgtgcaggtt ctttcagcgc
    29401 atcgccaatg tttttcataa aggtcatccg gccctgctgg tttttctcat acagacgctg
    29461 cgcctgcttg agtttcttgt ttaccagcac ttcgcgatcc tgatcgcgaa tgatgaaaat
    29521 ttgtgtgcgc ggcgcgacct ggctgcggaa catgcggatc tcatacagct cgttattgat
    29581 cgtcgcctga ataatcccct gatgctgttc cgccatggtg gtgatgtttt gcagattcaa
    29641 atgcggcagc aaatgatcgg caattttgtt acttatgaca gtgcggttcg attcctgatc
    29701 gtgaaccagc aggccgagcg gcagcagtga gactatctct tcattgattg cccgtaaaat
    29761 gcgcaattcg ttattgaccg ccgtgttggg tagactttct gtactacgac tggagaaatg
    29821 gcggaatgtg gtatagccaa ataacgccag cgccagcaaa ccgatgttca gcagcagtgg
    29881 cagcagaatg ttttgcaacg tatccagcaa taaggtgcca taaggaacct gccagaccag
    29941 gcgcatatcg gtagagttga gtgccgaggc gatttcaatc ttcgtactgt taaagtggat
    30001 actgacgcta tccgtccctt ctttctcatt atcattgttt cccgttgccg tcgcgtcttg
    30061 ctcaaggcgg aaactgtcca gcggcatacc cggtgggatc aaatcattaa tcggcagatc
    30121 aaaagccacg accgttgcca gatgtcccgg ctggttgaac gtggtacgca aggtaaagta
    30181 atgaccgttc tgccaggcca ggcggcgcat gttagaaaaa ctttcgcgtt catcgagggc
    30241 gttggcctgt tgcaacattt ctgcacgacg tgaatcaaca atgtcactga cggtcgattc
    30301 tttaaatccg gaggtgagat ctttgagggg cagggttgag atcagcacca gactgttatc
    30361 ctgaccattc aggtaataca ttgaccacgg tacattttct gcgccccaca atgtgtccag
    30421 ataggtggac atccgttgag tcatctcaag cgttgagttg tcgtgagagc caaagattaa
    30481 cgcttccgtt ttgcggcgcg gtttttccag atagtagaca tcctgtttca ggcgcgtctc
    30541 ttgtaaacct tcgccggagg agggggaggt cgtcgcggca atattgtcgt agatctgcca
    30601 ggtcacgtaa cgccaggtat cgacgcgctt ttggatagcg tgggtaatgt cgacaatctg
    30661 gtaactttta tctttcagcc aggcgttaac ggcgctttgt accattacac ccatcgtcac
    30721 cagtaacaca atgatcaaca gtaaaaagaa gcgggtaatg ctccccggta ggagtgaaaa
    30781 gcgggtcgtg gccgttgtct ctttctgacg cattcgtgtt tatgacctgt taaaacttcg
    30841 cgaagtgata gggcagtata aagggtacag tgtgaaattc cagactctta cctgattgat
    30901 gattctctat tctcccgtga tatttatcat cctgcattgg gggaaataga tgtacaagcg
    30961 ccattttgct gtacaaataa cctacaaaaa gcccaacaaa aataattgca aataaacaaa
    31021 gattgctgga aattatgctg atgttaatta tttgtgaaat agttaacaag cgttatagtt
    31081 tttctgtggt agcacagaat aatgaaaagt gtgtaaagaa gggtaaaaaa aaccggatgc
    31141 gaggcatccg gttgaaatag gggtaaacag acattcagaa atgaatgacg gtaataaata
    31201 aagttaatga tgatagcggg agttattcta gttgcgagtg aaggtttt
//
LOCUS       DAFNBJ010000047        30997 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000047 DAFNBJ010000000
VERSION     DAFNBJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.047"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="L2X49_001959"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="L2X49_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBN4797048.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="L2X49_001960"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="L2X49_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBN4797049.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="L2X49_001961"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="L2X49_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBN4797050.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="L2X49_001962"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="L2X49_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBN4797051.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="L2X49_001963"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="L2X49_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBN4797052.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="L2X49_001964"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="L2X49_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBN4797053.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="L2X49_001965"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="L2X49_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBN4797054.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="L2X49_001966"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="L2X49_001966"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBN4797055.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="L2X49_001967"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="L2X49_001967"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBN4797056.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /locus_tag="L2X49_001968"
     CDS             12889..13428
                     /locus_tag="L2X49_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HBN4797057.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="L2X49_001969"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="L2X49_001969"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBN4797058.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="L2X49_001970"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="L2X49_001970"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBN4797059.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETCAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="L2X49_001971"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="L2X49_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBN4797060.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="L2X49_001972"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="L2X49_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBN4797061.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="L2X49_001973"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="L2X49_001973"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBN4797062.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="L2X49_001974"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="L2X49_001974"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBN4797063.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="L2X49_001975"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="L2X49_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBN4797064.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="L2X49_001976"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="L2X49_001976"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBN4797065.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="L2X49_001977"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="L2X49_001977"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBN4797066.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="L2X49_001978"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="L2X49_001978"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBN4797067.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /locus_tag="L2X49_001979"
     CDS             22607..23197
                     /locus_tag="L2X49_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBN4797068.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="L2X49_001980"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="L2X49_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBN4797069.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /locus_tag="L2X49_001981"
     CDS             23669..24364
                     /locus_tag="L2X49_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBN4797070.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="L2X49_001982"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="L2X49_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBN4797071.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="L2X49_001983"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="L2X49_001983"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBN4797072.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="L2X49_001984"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="L2X49_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBN4797073.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGHFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLTKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="L2X49_001985"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="L2X49_001985"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBN4797074.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="L2X49_001986"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="L2X49_001986"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBN4797075.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7309 a         8042 c         8477 g         7169 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gtatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcgggttaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggggcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gtactgggtc tgtactacat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaagggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt actcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtggggg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acagaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt gcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gaattgcgag gcatttagcg
    14881 cccgctctac gtcaacagcc gttttgccgt tgatacgcag gcggatgtca tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg ctacggcaat
    15121 cgccgtattc actaacaccg cgtcggcccc catttccagg gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg aactgtggcc tgctggataa taatctccag
    15241 catggcacag gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcatttc ggcggctttc agagtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc caattttagc cagttagtgc ctaacgcttc
    15481 acgtgccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cagcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggcg ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgccgagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacctcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggcgtga tgatgccctg gcgggcgtag tgcagttggg tgacacggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgccatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagccg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggcagtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta gtgcgtgaaa acgacggtcg
    21541 ccgcgtaccg gtcacgctgt ttaccaaagg cggcggacag tggctggaag ccatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggac atcgccgatg cgcgccgccg
    21661 tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc
    21721 tgcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agttgcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc ctcgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acgtctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccgctgat ggataaaggc gagcagttgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgtattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgagagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    23761 tagccacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctga tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatctcg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    24901 cctgctgcgg gtagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 cgcaggaaat gcagcagtta catcctggag atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattatcc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtgttgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctg ttgcaggatg aaatgaagcg caaagagaag ctggtagctc
    25741 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa tccactttcc tcaattaaag
    25801 ggctggcgaa atactttgcc gaacgcgcgc cagcaggggg agaagcgcat caattggcgc
    25861 aggtgatggc gaaagaagcc gaccgtttaa accgcgtggt aagcgagttg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aagccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg aacaggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 ggagatggac ggcatcgcca cgctgaaaga gatcaaagcg ttaaacccgg caattccggt
    26641 gctgattatg actgcgtact ccagcgtcga gacggcggta gaggcactga aaactggggc
    26701 gctggattat ctcatcaagc cgctggattt cgacaccctg caggcgacgc tggaaaaggc
    26761 gctcgcgcat acgcacagta ttgatgctga aacacctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagctcgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acggtactga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgcggc
    27001 actcaacgaa tccttgctgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcat tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aagatatccc
    27361 tctgctggct ggccattttc ttcagcgctt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ctttacgcca caggcgatgg atttgctgat ccactacgac tggccgggaa atattcgtga
    27481 actggaaaac gcggtggaac gggcggtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccggtacgcc gatcccgctg gggcaaagtc aggatattca
    27601 gccgctggta gaagtcgaaa aagaagtgat tctggcggca ctggagaaaa cgggcggcaa
    27661 caaaaccgag gccgcccgtc agttagggat cacgcgcaaa acgctattga caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccaata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgttatccgc
    27901 cagtttcgtg cccgcgtgga acactttgcc gtcagccact tcttccagcg gcaggccgtg
    27961 gatcacatcg ccggtgcggt aatcgcccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgt tcatcccact cggatgtttt ctcgtccagc ttcccttcac aggctgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ccggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28321 gcccatcccg cccgtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtagccatc ggcaacacat gctcgccgtc caccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacaat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggtcacgcca atagcaacgt tttgcagcgc gggttccagc gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtctcaa ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaatca ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatgg aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcag cagctttaat accggcgatt ttcgcggagt acacgcggct catctggccc
    29281 gcgccaatac cgatggtcat attgtttttg gcatagacga tagcattgga tttcacgaac
    29341 ttcgccactt tccagcagaa cagcgcatcg cgcagttcct gttcggtcgg ctgacgttgg
    29401 gtgacgacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgctt ggcggcggtg attttcaggg cttcttcgct ggcggaaggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatccagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gctacaccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tcctgcttct taatgaagtt caggttcagc gtgcgtggga agcgaccagc ggcttctttg
    30001 ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt agcggcggag cgcaccatgg tcgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttatttttcg cccgcattgt
    30721 aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt caatggtgat cacaattttg
    30781 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    30841 gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc agtcagtcat ttttctgcaa
    30901 tttttctatt gcggcctgcg gagaactccc tataatgcgc ctccatcgac acggcggatg
    30961 tgaatcactt cacacaaaca gccggttcgg ttgaaga
//
LOCUS       DAFNBJ010000048        30460 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000048 DAFNBJ010000000
VERSION     DAFNBJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.048"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..418)
                     /locus_tag="L2X49_001987"
     CDS             complement(<1..418)
                     /locus_tag="L2X49_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBN4797076.1"
                     /translation="MSNLATVTHLRPSQRPVERRVAEVEDGYTRLANALYEELIGADL
                     TKNQSKVAHAICRKTYGYGKKMDRISDSQLAQITRLPRQKVNKAKNELIAMKVILREG
                     QQIGPNKNIEEWQIEGCHYSGDNVTALVTKSVTKTVT"
     gene            complement(411..557)
                     /locus_tag="L2X49_001988"
     CDS             complement(411..557)
                     /locus_tag="L2X49_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HBN4797077.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            complement(592..873)
                     /locus_tag="L2X49_001989"
     CDS             complement(592..873)
                     /locus_tag="L2X49_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797078.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLANYLTKEKAPKNGEFFEA"
     gene            complement(990..1205)
                     /locus_tag="L2X49_001990"
     CDS             complement(990..1205)
                     /locus_tag="L2X49_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4797079.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVECLNKLGAKVSLDDVFPPEHKAA"
     gene            1281..1976
                     /locus_tag="L2X49_001991"
     CDS             1281..1976
                     /locus_tag="L2X49_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBN4797080.1"
                     /translation="MDGFSTERNNKGMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLDEIGVVFKYLGIDNVSFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERWVSTTKKASDCAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLHPLNPQYPMIPCNDSCSVVG
                     KVIASQWPEETFS"
     gene            2311..2643
                     /locus_tag="L2X49_001992"
     CDS             2311..2643
                     /locus_tag="L2X49_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HBN4797081.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEIGVTCVGRQKMKL
                     GSKPLYEG"
     gene            2647..2898
                     /locus_tag="L2X49_001993"
     CDS             2647..2898
                     /locus_tag="L2X49_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797082.1"
                     /translation="MEFHESAIYDFRANANSVKPQPIAVLFKTMGAWAVLCFASDDTD
                     ARMAIGQEMEVDPTNDKFIIYGAPSNYLLDTCNIYNKAA"
     gene            2926..3168
                     /locus_tag="L2X49_001994"
     CDS             2926..3168
                     /locus_tag="L2X49_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797083.1"
                     /translation="MKIKTMAASPLSGRIFQGTLNTEKGMWVGKKEDVTEQAIKAVTE
                     HLMIKDRKYAYETNDGKWVIIMHQLVDELPEEFIAD"
     gene            3334..3519
                     /locus_tag="L2X49_001995"
     CDS             3334..3519
                     /locus_tag="L2X49_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797084.1"
                     /translation="MCEFYEADIKHPEMASDATLRDYFAAKFMQGVCANPDRLYDNEA
                     LAKEAYAMADAMLKARE"
     gene            3654..4622
                     /locus_tag="L2X49_001996"
     CDS             3654..4622
                     /locus_tag="L2X49_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HBN4797085.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSSDQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVRDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRKREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAERERIEAEQRAQ
                     REAKEAAERAEREKRAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            4647..4793
                     /locus_tag="L2X49_001997"
     CDS             4647..4793
                     /locus_tag="L2X49_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HBN4797086.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRLIDVLNQPGV
                     PKNG"
     gene            4786..4899
                     /gene="kil"
                     /locus_tag="L2X49_001998"
     CDS             4786..4899
                     /gene="kil"
                     /locus_tag="L2X49_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HBN4797087.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVEAYRKWRSK"
     gene            4896..5084
                     /locus_tag="L2X49_001999"
     CDS             4896..5084
                     /locus_tag="L2X49_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797088.1"
                     /translation="MIPVELAKTPELSRLKREYHIAEARYWRKAGDKSKKQLCLWQAQ
                     RERMNEREFLSSQSELPF"
     gene            5093..5800
                     /locus_tag="L2X49_002000"
     CDS             5093..5800
                     /locus_tag="L2X49_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HBN4797089.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIKIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKQGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            5801..6274
                     /gene="ssb"
                     /locus_tag="L2X49_002001"
     CDS             5801..6274
                     /gene="ssb"
                     /locus_tag="L2X49_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBN4797090.1"
                     /translation="MASRGVNKVIIIGRLGHDPEIRYSPSGTAFANLTVATSEQWRDK
                     QTGEQKEQTEWHRVVMSGKLAEIASEYLRKGSEVYLEGKLRTRKWQDQSGQDRFTTEV
                     IVGVGGTMQMLGGKQGGNEQSSHQRNNGQQQRQQSQQQGNHSEPPMNFDDSDIPF"
     gene            6772..7320
                     /locus_tag="L2X49_002002"
     CDS             6772..7320
                     /locus_tag="L2X49_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBN4797091.1"
                     /translation="MNHLMVDLETMGNGPYAPVISIGAVFFDPNTGETGEEFSVNISL
                     ESSMRYRARPDASTILWWMEQSEEARKSLTSNTQELSTALSWLSEFIIKNANHKFVQV
                     WGNGASFDCVILRNSYSLTGQPVPWQWWNDRDVRTIVELGKVIGFDPKRDMPFKGTRH
                     NALDDAIHQAKYVSAIWKKLAK"
     gene            7337..7633
                     /locus_tag="L2X49_002003"
     CDS             7337..7633
                     /locus_tag="L2X49_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HBN4797092.1"
                     /translation="MPAPLYGADDPRRCSGNSISEVLENIKNNLDAFLALPPETKEER
                     KYRRDIQLAEKQEKDRINETSIRPFRKSIYTHFPEYIDPRLRNYRSRYGAISND"
     gene            7644..7808
                     /locus_tag="L2X49_002004"
     CDS             7644..7808
                     /locus_tag="L2X49_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HBN4797093.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMLRSGKK"
     gene            7805..8224
                     /locus_tag="L2X49_002005"
     CDS             7805..8224
                     /locus_tag="L2X49_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797094.1"
                     /translation="MKQMSLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKCA
                     ALAAENAALKQSEKEFNNFCRQEYYGWEDNFTETPATDAFLAEVRAQGVEMYADNLDN
                     GADDAERGGFDYAVKFLRSEASGVRLFADQLRKGGNQ"
     gene            8221..8766
                     /locus_tag="L2X49_002006"
     CDS             8221..8766
                     /locus_tag="L2X49_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HBN4797095.1"
                     /translation="MSKINYQALREKAEKATCGVWSLEYGEERFDAGDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDERERNQQYIKLRDQE
                     NEEIALTVGKLRVELEAAEKRIAELQLREVVLPQCYSMLHRVDFDEPYHTEMVYRQHQ
                     VLEALHNAGINVTEAGKGEAS"
     gene            8763..9317
                     /locus_tag="L2X49_002007"
     CDS             8763..9317
                     /locus_tag="L2X49_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797096.1"
                     /translation="MSTIPKERLEQLASGNAWYCVQDDEAAELARIALASLEAKPVAY
                     IFKHPAGKLFWALTDESNKDQSDVIPVYAASPAPIASEAIENAIEYIRSIAFHIDEDD
                     YHGKHIAYFMRQALAWLEGHSCSDDSQGKSDNSPLPRYQVIELTMLVKQLVSQLKKAK
                     PDCKLPDRAMDYLLRNELVSAEGV"
     gene            9320..9487
                     /locus_tag="L2X49_002008"
                     /pseudo
     CDS             9320..9487
                     /locus_tag="L2X49_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            9656..9898
                     /locus_tag="L2X49_002009"
     CDS             9656..9898
                     /locus_tag="L2X49_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001455577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBN4797097.1"
                     /translation="MRNYPVNPDGWISCSERMPDTKTAVLVAKEFDRKGDWRMKWATY
                     IPGHPDANDGWIIPGASWKPSHWMPLPEPPQEVNRG"
     gene            9998..10297
                     /locus_tag="L2X49_002010"
     CDS             9998..10297
                     /locus_tag="L2X49_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797098.1"
                     /translation="MACSTFNHLTLQKYQTDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAVRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            10406..10606
                     /gene="torI"
                     /locus_tag="L2X49_002011"
     CDS             10406..10606
                     /gene="torI"
                     /locus_tag="L2X49_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HBN4797099.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(11136..12383)
                     /locus_tag="L2X49_002012"
                     /pseudo
     CDS             complement(11136..12383)
                     /locus_tag="L2X49_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
     gene            complement(12455..13369)
                     /locus_tag="L2X49_002013"
     CDS             complement(12455..13369)
                     /locus_tag="L2X49_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HBN4797100.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRMLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            13585..15018
                     /gene="cscA"
                     /locus_tag="L2X49_002014"
     CDS             13585..15018
                     /gene="cscA"
                     /locus_tag="L2X49_002014"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBN4797101.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(15026..16084)
                     /locus_tag="L2X49_002015"
     CDS             complement(15026..16084)
                     /locus_tag="L2X49_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBN4797102.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPHDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            16264..16482
                     /locus_tag="L2X49_002016"
                     /pseudo
     CDS             16264..16482
                     /locus_tag="L2X49_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            16500..17828
                     /gene="dsdA"
                     /locus_tag="L2X49_002017"
     CDS             16500..17828
                     /gene="dsdA"
                     /locus_tag="L2X49_002017"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBN4797103.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(17936..19474)
                     /gene="emrY"
                     /locus_tag="L2X49_002018"
     CDS             complement(17936..19474)
                     /gene="emrY"
                     /locus_tag="L2X49_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBN4797104.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGIG"
     gene            complement(19474..20637)
                     /gene="emrK"
                     /locus_tag="L2X49_002019"
     CDS             complement(19474..20637)
                     /gene="emrK"
                     /locus_tag="L2X49_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBN4797105.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQF"
     gene            21053..21667
                     /gene="evgA"
                     /locus_tag="L2X49_002020"
     CDS             21053..21667
                     /gene="evgA"
                     /locus_tag="L2X49_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBN4797106.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            21672..25265
                     /gene="evgS"
                     /locus_tag="L2X49_002021"
     CDS             21672..25265
                     /gene="evgS"
                     /locus_tag="L2X49_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBN4797107.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(25321..26466)
                     /gene="yfdE"
                     /locus_tag="L2X49_002022"
     CDS             complement(25321..26466)
                     /gene="yfdE"
                     /locus_tag="L2X49_002022"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBN4797108.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAAMLDQHGEQIRQEFSS"
     gene            complement(26540..27484)
                     /gene="yfdV"
                     /locus_tag="L2X49_002023"
     CDS             complement(26540..27484)
                     /gene="yfdV"
                     /locus_tag="L2X49_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBN4797109.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(27554..29248)
                     /gene="oxc"
                     /locus_tag="L2X49_002024"
     CDS             complement(27554..29248)
                     /gene="oxc"
                     /locus_tag="L2X49_002024"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBN4797110.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(29302..>30460)
                     /gene="frc"
                     /locus_tag="L2X49_002025"
     CDS             complement(29302..>30460)
                     /gene="frc"
                     /locus_tag="L2X49_002025"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBN4797111.1"
                     /translation="DVIKIERPGVGDVTRHQLRDIPDIDALYFTMLNSNKRSIELNTK
                     TAEGKEVMEKLIREADILVENFHPGAIDHMGFTWEHIQEINPRLIFGSIKGFDECSPY
                     VNVKAYENVAQAAGGAASTTGFWDGPPLVSAAALGDSNTGMHLLIGLLAALLHREKTG
                     RGQRVTMSMQDAVLNLCRVKLRDQQRLDKLGYLEEYPQYPNGTFGDAVPRGGNAGGGG
                     QPGWILKCKGWETDPNAYIYFTIQEQNWENTCKAIGKPEWITDPAYSTAHARQPHIFD
                     IFAEIEKYTVTIDKHEAVAYLTQFDIPCAPVLSMKEISLDPSLRQSGSVVEVEQPLRG
                     KYLTVGCPMKFSAFTPDIKAAPLLGEHTAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         9014 a         6936 c         6858 g         7652 t
ORIGIN      
        1 ctgtcaccgt tttggtgaca ctttttgtca ccaatgcagt gacattatca ccagagtagt
       61 gacacccttc gatttgccat tcttcgatgt tcttgttagg cccgatttgc tggccttcgc
      121 gaaggataac cttcatcgcg ataagctcat tcttggcctt gtttaccttc tgtcttggca
      181 gcctggtaat ttgagctaac tgactatcag agatgcgatc catcttttta ccgtagccgt
      241 atgttttacg gcatatggcg tgggcaacct tgctctgatt tttcgttaaa tctgcgccga
      301 taagctcttc atacagggca tttgcaagac gggtataacc atcttcaact tctgccacac
      361 gacgctccac aggccgttgt gaaggcctta aatgtgttac ggttgcaaga ttactcatga
      421 cctttctcct tctgcatcag cttcactttt tccaactcag cccggaatcg accaggctgc
      481 ttgaagctgg acaggaagcg atcacgtagt atgtgtttgt gaattttgtc ctggtaagga
      541 ctgagttgtt ttgtcataat tactcctgtg gattgatcca gtctttctac atcaggcctc
      601 gaagaattcg ccgttcttcg gggctttttc ttttgtcagg taattggcaa gccgcttagt
      661 caattcagcc atttcatcgt cttcgattcc gtattccaga acagccagca tcatgcttac
      721 ctgcgagaag aaaccattct tccatcggct tacctgatat tcaggaaccc ccattgctcg
      781 agcgaatgtc ttctgcccca tcagtgccag tttgttcagc aaggctgact cgatgcgagc
      841 cgctttcttg cttttagttg caatagtacc catagataat ttccttaatg attagataga
      901 gttggcttcg caaagaaacg caaaaccata gagatttgtt tctggtaatg ccctttttca
      961 gggctgtgat gtgtaagagc tagaatgtct taagcggctt tgtgttccgg cgggaacacg
     1021 tcatcaagac tgacttttgc gcctaacttg tttaggcact caacaagagc acggcatgtt
     1081 ttaaggtctg ggaagcgacg accagattcc caatgtccga tagctccctg tgtgcatcca
     1141 actgccttag caagtgttgt ttgagagata ttcagtgact ctcgatattt tcgtaggttg
     1201 ctcatatgcc ctccatagta accatgaaat aataatacga tatgtacttt tagaatgcaa
     1261 acaaaaaata catcttgtgc atggatggtt ttagtacaga gcgtaataat aagggtatga
     1321 aaatgaaatg gtatgaactg gctagatcca gaatgaaaga gctcggcata actcaagaga
     1381 agttagctga agagcttggt atgacgcagg gtggaattgg tcactggttg cgcggatctc
     1441 gtcatccatc tcttgacgag attggtgtgg tgtttaaata ccttggtatt gataacgtct
     1501 cattcaacca cgacggtaca ttttcacctg ttggcgaata ctcatctgcc cccgttaaaa
     1561 aacaatatga gtaccctgtt ttttctcatg ttcaggccgg gatgttctcg cctgaactta
     1621 gaacctttac caaaggtgat gcggagagat gggtcagcac aaccaaaaaa gccagtgatt
     1681 gtgcgttctg gcttgaagtt gaaggtaatt ccatgaccgc gccaacagga tccaagccaa
     1741 gctttcctga cgggatgtta attctcgttg accctgagca ggctgttgag ccaggtgatt
     1801 tctgcatagc cagacttggt ggtgacgagt ttaccttcaa gaaactgatc agggatagcg
     1861 gtcaggtgtt cctacatcca ctaaacccgc aatatccaat gatcccatgc aatgatagct
     1921 gttccgttgt agggaaagtt atcgccagcc agtggcctga agagacattt agttaacagc
     1981 ctcaccactc taaagcacac aacaataacc cgaccttagt gtcgggtttt ctttttccaa
     2041 aatataaacc cattaaatac aaagcgttat aaaaaaccaa ttatatttag aacattttgt
     2101 attgactcga taaagtacaa atcgtactat ttagccatca gcaggacgca ctgaccacca
     2161 tgaaggtgag gctcttaaaa atttagccct gaagaagggc agcattcaaa gcagaaagct
     2221 ttgagtagcg cgaaatgcag ctgcaagaca gcaaccgtgg agataagcat cacggcgcgt
     2281 tactcaaagc taactgacag gagaatccgg atggatgcac aaacacgccg ccgcgaacgt
     2341 cgcgcagaga aacaggctca atggaaagca gcaaatcccc tgttagttgg ggtaagcgca
     2401 aaaccagtta accgccctat tctctcgctg aatcgcaaac cgaaatcacg agtagaaagc
     2461 gcactgaatc cgatagacct tacggtgctg gctgaatacc acgaacagat tgaaagcaac
     2521 ctgcagcgta ttgagcgcaa gaatcagcgc acatggtaca gcaagccacg cagtgaaatt
     2581 ggtgtgactt gtgttggtcg ccagaaaatg aaattaggca gcaaaccact gtatgagggg
     2641 tgatatatgg aatttcatga aagtgcgatt tatgattttc gcgctaacgc aaattcagta
     2701 aaaccacagc caattgcagt tctttttaaa acaatgggtg cgtgggctgt tttatgcttc
     2761 gcctctgatg acactgacgc aagaatggca ataggccaag agatggaggt ggacccgaca
     2821 aacgataaat tcataattta tggcgctcca tctaattact tacttgatac ctgcaacatt
     2881 tacaacaagg ctgcctgatg gcggcattta tttttaagga tctatatgaa aattaaaact
     2941 atggcagcaa gcccattaag cggtcgtatt tttcaaggaa cattaaacac tgaaaaaggg
     3001 atgtgggtag gaaagaaaga agatgtcacc gaacaagcga ttaaggcagt aactgaacac
     3061 ctgatgataa aagaccggaa atatgcatac gaaacgaatg atggcaaatg ggtgataata
     3121 atgcatcaac tggttgatga attaccagaa gagtttattg ctgattaaaa ttatttttgc
     3181 ataaacaaca gaggtgaata tgaacgcagt tggatttaca aaatggatgg cagagcaaga
     3241 tatcacagga gccgacgaaa aggctgtgta ctacatggct atgctatgga ttcacaaagc
     3301 aaaagagatt gcaaatgctc ttggaggtga gtgatgtgcg agttttatga agcagatatc
     3361 aaacacccag aaatggcaag tgatgcgaca ttacgtgatt acttcgcagc aaagttcatg
     3421 cagggagttt gtgcaaatcc agacaggctt tatgacaacg aagcacttgc taaagaagct
     3481 tatgcgatgg cagacgcaat gctcaaagct cgcgaataag cactgtgtat tcattccaac
     3541 gagtgaatac acggagcaat gtcgctcgta actaaacagg agccgacttg ttctgattat
     3601 tggaaatctt ctttgccctt cagtgtgagg gcaatttttt tgacggagga tatatgagtg
     3661 aagtaacaga tttagttgtt attgaaaaag caaatgcaat gactgtattt cagtcttccg
     3721 accagattga agaaatcctt caaaaggttg aacgtgaagt tatgtccttt gtgcctgata
     3781 tcacaacggc aaagggcaga aaggagatcg cttctctggc gtataaagtt gcgcagacga
     3841 aaacatatct cgatggtctt ggcaaagacc ttgttgctga actgaaggaa attccaaagc
     3901 taattgatgc caaccgcaag acagtgcgtg atcgccttga tgagctgaaa gccaaggcgc
     3961 gccagcctct tactgattat gaggaggaac aggcacggat taaagccgaa gaagaagcta
     4021 aggcagcagc tgaagctctc gcaaagcaaa ttgagtctga ccatgaaata gcgattttga
     4081 tggatcgcga atttgaccgc aaaagagaag aggcaagact caaagcggag caggaaaagc
     4141 gagagcatga agaacgctta aaaagagaag ctgaagagaa agccagagca gaagccgaag
     4201 caaaggcaaa agccgaaatt gaagcagcag caaggcgaga agcagaagct aaggcagcag
     4261 cggaacgtgc agagcgtgaa cgcattgaag ccgagcaacg agcacagcgc gaagcaaaag
     4321 aggcagcaga acgagctgaa agagaaaagc gggcggcaat tgaagcagaa cgccgaaaag
     4381 cacaggagga ggctgaacga atccggcgcg aagctgaagc aaaagagcaa gccagaatag
     4441 cagaagaaaa aagaatcaag gacgaagaag agcgtagagc aaaggataaa gctcaccgga
     4501 aagaagtaaa taacaaaata cttgctgacc ttatcaaggt tggcgcatca gaagatgttg
     4561 ctaaaaatat cataacagcc atcgtaaaag gcgaagtatt cgcaacaaaa ataacctact
     4621 aataaaacca acataaggaa ccacccatga tttacgcaat cgcgggaggc gctcgcatgg
     4681 gtgccttcca attaaatgaa tctttacttg aacgaatcac ccgtaaatta cgtgacggat
     4741 ggaaacgact catcgacgta cttaatcagc caggagttcc aaaaaatgga taaaacactt
     4801 atggctattc agactaaatt cactatcgcc acttttattg gcgatgaaaa gatgtttcgt
     4861 gaggccgtcg aagcctacag aaaatggagg tctaaatgat tccggtagag ctggcgaaaa
     4921 ctccagagtt aagtcgatta aaaagagaat atcacattgc tgaggctcgt tactggcgta
     4981 aagcgggaga taaatcaaag aaacaacttt gtttatggca agcacaaaga gagcgcatga
     5041 atgagcgcga atttctttcc tcccaatccg aattaccatt ctgaggtgaa ttatggattt
     5101 gaataaattc gatgagccat tcagccccga agatatcgaa tggcgaatac agcaaagcgg
     5161 taaaacacgc gatggcaagg tgtgggctat ggtgctggct tatgtcacga accgggcaat
     5221 catgaaacgc ctggacgatg tttgcggcaa agcaggatgg cgcaatgaat accgcgatat
     5281 tcccaacaac ggcggcgttg aatgcggcat atcaataaag attgattccg aatgggtaac
     5341 caaatgggat gctgctgaaa acacgcaggt agaagccgtc aaaggtggtc gttccggtgc
     5401 aatgaagcgc gctgccgttc aatggggaat cggtcggtat ctgtataacc ttgaggaagg
     5461 tttcgcacaa acatctctcg ataaaaagca gggatggcac agggcaaaac tcaaggatgg
     5521 aacaggattt tactggctcc ctccatcgct gccgggatgg gcaatcccag catcagataa
     5581 caaaccatca ccagaaaata ccaaccagaa atctccatcg gttgactgcg aacaaatcct
     5641 gaaagacttc agcgattatg cgtcaacaga aactgacaag aaaaaactca tcgagcgtta
     5701 tcagcgtgac tggcaattaa tggctggcaa cgaggaggcg caggctaaat gcgttcaggt
     5761 aatgaacatc agagttaacg aactaaaaca ggcggcataa atggcaagca gaggcgtaaa
     5821 taaggtgatt attattggtc gccttgggca tgatccagaa atcagatatt caccatcagg
     5881 aacggcattt gcaaacctta cagttgctac gtcagaacaa tggcgtgata agcaaactgg
     5941 agagcaaaag gagcagacgg agtggcaccg cgtggtaatg agcgggaaac tggcagaaat
     6001 tgccagcgaa tatctgcgaa aaggctctga ggtttatctt gaaggcaaat tgcggacaag
     6061 aaaatggcag gatcaaagtg gacaggatcg gttcactacc gaagtcatcg tgggcgttgg
     6121 tggaaccatg caaatgcttg gtggcaaaca aggaggcaat gaacagtctt cacatcagcg
     6181 aaataacggt cagcaacaaa gacagcaatc tcagcagcag gggaatcaca gcgaaccacc
     6241 tatgaacttc gacgattcgg atattccgtt ctaggagctg aatatgaaag tctgctcaag
     6301 atgccatcaa cagaaggaag aaagggactt tcaaatcaga agagcatcca gagatggatt
     6361 aactgccgct tgccgggctt gcctggctga atacgacaaa gaacgcgctg gattgccaca
     6421 tcgagtatca gcaaggagag aatatcaatc atcggaacgc ggaagagaac ggtgtaacgc
     6481 agccaaaaag cggttcattc agagcaaccc atggaaaaga aaagcccaca tcattgtggg
     6541 taattttttg cgcgacggta agctaatccg accaccacaa tgtgagtgct gcggatcaga
     6601 atgtaaacca caggcgcacc actgcgacta cagcaaacca accgatgtga tgtggctctg
     6661 caagtcatgt catgtcgagt ggcacaaaca taacaaacct atctacccag acgaggaacc
     6721 agtaactctc cccttccctc gtcacgctat tcacgcaatt taaggactta catgaatcac
     6781 ttaatggttg accttgaaac aatgggcaac gggccatatg cgccagttat ttctattggt
     6841 gcggtattct ttgacccgaa taccggagaa acaggagaag agttctcggt aaatatctcg
     6901 cttgagtcat caatgcgata tcgggcgcgt cctgacgctt caacgatttt atggtggatg
     6961 gaacagagtg aagaagccag aaaatcgcta accagcaaca ctcaagagct ttcaacggct
     7021 ctttcatggt tatctgaatt catcataaag aacgctaacc acaaattcgt tcaggtttgg
     7081 gggaatggag catcatttga ctgcgttatt ctccgaaaca gttattcact gacagggcag
     7141 ccagttccgt ggcagtggtg gaatgaccgc gacgtaagaa caatcgtcga acttgggaag
     7201 gtaataggat tcgaccctaa gcgagatatg ccattcaaag gaactcgcca caacgcgctt
     7261 gatgatgcca ttcaccaagc caaatacgtt tcagcgatct ggaaaaagtt agctaaataa
     7321 tcaacaggag aaaaccatgc cagcgcctct atatggtgcg gacgacccgc gccgctgttc
     7381 cggcaattcc atatcggagg tgctggaaaa tatcaagaat aatctcgacg cgtttcttgc
     7441 tctgccaccg gaaacaaaag aagaacggaa gtaccgacgc gatatacaac tcgcagaaaa
     7501 acaggaaaaa gaccgaataa acgaaacatc aatccgacca ttccgcaaat ccatatatac
     7561 ccacttccct gaatatatcg acccgcgcct gcgtaattac cgctcacgct atggcgctat
     7621 cagtaatgac tgaggaattt accatgagag gacttgcata caatcccggc attcttccgg
     7681 cagaaatgat tattcgccaa cgcgtaaagc caatgccatc gagagaggaa ttgcttaaga
     7741 gaaattcttt tccatcagtg aatcaaaaca aatatctgaa tgcgatgttg cggagtggga
     7801 agaaatgaaa caaatgtcac taattgagat ggatggtttt ctgaaaggta aatgcatccc
     7861 atgtgattta aaggttaacg aaacaaacgc tgaatatctg gtgcgtaaat ttgctgaagc
     7921 ggaggccaag tgcgcggcgc tagcagcgga gaatgcggcg cttaaacagt cggagaagga
     7981 atttaataac ttctgtcgtc aggagtacta cggttgggag gacaacttca cggaaacccc
     8041 agccaccgat gctttcctgg ctgaagtacg ggcgcagggg gtagagatgt acgcagataa
     8101 cctcgacaac ggagcagacg acgcagaacg aggtggtttt gattatgccg ttaagtttct
     8161 acgcagtgaa gcgtctggtg tacgtttgtt cgccgaccag cttcgcaaag gaggcaacca
     8221 gtgagcaaaa ttaattacca ggcactgcgt gaaaaggcag agaaagcaac gtgtggtgtg
     8281 tggtcgctcg aatatggaga ggagagattt gatgctggtg atgcgctaat tcatcgtgaa
     8341 gttgttggat atcttcccat ttgcagaatt gaaggagcgc atccagaaag cggtttcgat
     8401 gaagatttcc aaatggaaca gcaggccaat gctgaattca tcgccgcagc caatccggct
     8461 accgtcttgg cgctgctgga tgaacgggaa agaaaccagc aatacatcaa actccgcgat
     8521 caggagaacg aggaaattgc gctaacggta gggaaactgc gtgttgagct ggaagccgca
     8581 gagaagcgca ttgctgagct gcagttgcgg gaggttgtgc ttccgcaatg ctatagcatg
     8641 ttgcatcgcg tcgattttga cgagccttac cacactgaaa tggtttacag gcagcatcag
     8701 gttcttgagg cactgcacaa cgctggaata aacgtcaccg aagcaggtaa aggagaggca
     8761 tcatgagcac tattcccaaa gaacgtctgg aacaattagc atctggaaac gcatggtatt
     8821 gcgttcaaga tgacgaagcg gctgagctgg cgcgtatcgc gctggcatcg ctggaagcaa
     8881 aaccagttgc ttatattttc aaacatccgg ccgggaaatt attctgggct ttgacggatg
     8941 aaagcaataa agatcaatcg gacgttattc ctgtttatgc tgcctcacct gcaccaatag
     9001 cgtcggaggc cattgaaaac gctattgaat acatccgcag tatcgctttt cacatcgatg
     9061 aagacgatta ccacggcaaa catattgcgt atttcatgcg acaagcattg gcctggctgg
     9121 aagggcattc atgcagtgat gacagtcagg gcaaatccga taattcacca ttgccgcgct
     9181 accaggtgat cgaattaaca atgctggtta aacaattggt cagccaactg aaaaaagcaa
     9241 aacctgattg caaattacct gatagggcga tggattatct tttgcgaaac gaactggtaa
     9301 gtgcggaggg ggtttaacga tgaccatttc tacaaaaaag cctctttatg ctgaggttca
     9361 caatgttcct gatgattatg agttcactga cgaggaacta aacagaatta ttgcaggtga
     9421 tatgttcact cctcgtcagg acgcaataat ggcacgggag atacagaaac tccgcgccgc
     9481 catgcttcat ggtgcagaac ctgtaaaaca gacttacaag ttgcctcccc tgccatccag
     9541 cgaagtaaac gaagcggcat ggagattaca caacatgctt actgaatgca tccctctaaa
     9601 tggtcatcag ttcaacaatc tgaagggttg gttctatgag gcgttaaagg tcgcaatgcg
     9661 caactatccg gtaaatccgg atggttggat aagctgtagt gagcgaatgc cggataccaa
     9721 aacagccgtt cttgttgcca aggagtttga taggaaaggt gactggcgaa tgaaatgggc
     9781 gacttacatc ccggggcatc ctgacgctaa tgatgggtgg atcattcctg gggcgtcgtg
     9841 gaaaccgtca cactggatgc cgctaccaga accgccgcag gaggttaacc gtggctaacc
     9901 tgcaacttgc cgttaaaggt gaataacaat cctcgcactc gcggggattt cttttatctg
     9961 aactcgctac ggcgggtttt gttttatgga gatgattatg gcctgttcaa cattcaacca
    10021 tctaacgtta cagaaatacc agacagaccc tgaagattta tgctcactgt gtggcggaaa
    10081 tcatggtaaa gccgccatga tcgaatgtaa ggacaaaatc cacatttgcc ttaattgcgt
    10141 tgatgtcctc gttgatatca aaaatgagag agaagataaa aagcgtagcg aggctgttcg
    10201 cgccttagat tcatggatga gggatggata tagtgccgcg caaatttatg acttagcaat
    10261 atcaaaaggc gaaataccag gagtgcgtat cgaataagac gtaaccaaca ttcgaattga
    10321 agaactgaaa gaacaccaag ccgcctgatg gcggtttttt atttcctgat ttgcaggttc
    10381 gattccctat tcggagatag cactcatgca acacgaacta caacctgatt cactggttga
    10441 tttgaaattc atcatggctg atactggctt cggtaaaacc ttcatctacg accggattaa
    10501 gtccggcgac ctgcctaaag ccaaagttat ccacgggcga gcaagatggt tatatcgtga
    10561 ccattgtgaa ttcaaaaata agctcttaag ccgcgccaat gggtaaaata gcgggtaaaa
    10621 tatttctcac atctaaaaaa caccattcca atcaatcccc tgcctcgtca agtagatgtc
    10681 tgcaggggac accagatacc cttcaaacga aatctacctt caccccgtaa aagatgggtt
    10741 tggcagcaca cttgccttat atctactcat ttttactgca acaggttgaa atctcagcac
    10801 tgtcagaaag cgctgatgac taaacagccc tgggccgggc gatgtaacca tcacacagaa
    10861 tcctgatagc gaaatatggc gtgactcgat acttcactcc gcaatgcatt ccttgatgaa
    10921 ttcgcaggac cgtgatacac gggacaggtc actgaatgac gacaatgtcc tggaaatcag
    10981 cgaaccgcgc atctgaagta catttgagcg actgtaccag aacatgaatg aggcgtttgg
    11041 attaggcgat tattagcagg gctaagcatt ttactattat tattttccgg ttgagggata
    11101 tagagctatc gacaacaacc ggaaaaagtt tacgtctata ttgctgaagg tacaggcgtt
    11161 tccataacta tttgctcgcg ttttttactc aagaagaaaa tgccaaatag caacatcagg
    11221 cagacaatac ccgaaattgc gaagaaaact gtctggtagc ctgcgtggtc aaagagtatc
    11281 ccagtcggcg ttgaaagcag cacaatccca agcgaactgg caatttgaaa accaatcaga
    11341 aagatcgtcg acgacaggcg cttatcaaaa tttgccacgc tgtatttgaa gacggatatg
    11401 acacaaagtg gaacctcaat agcatgtaac agcttcacta atgaaataat ccaggggtta
    11461 acgaacagcg cgcaggaaag gatacgcaac gccataatca caacaccgat aagtaatgca
    11521 ttttttggcc ctacccgatt cacaaagaaa ggaataatcg ccatgcatag cgcttcgagt
    11581 accacctgga atgagttgag ataaccatac aggcgcgttc ctacatcgtg tgattcgaat
    11641 aaacctgcat aaaagacagg aaaaagttgt tgatcaaaaa tgttatagaa agaccacgtc
    11701 cccacaataa atatgacgaa aacccagaag tttcgatcct tgaaaactgc gataaaatcc
    11761 tcttttttta cccctcccgc atccgccgct acgcactggt gatccttatc tttaaaacac
    11821 atgttgatca tcataaatac agcgccaaat agcgagacca accagaagtt gatatgggga
    11881 ctgatactaa aaaatatgcc ggcaaagaac gcgccaatag catagccaaa agatccccag
    11941 gcgcgcgctg ttccatattc gaaatgaaaa tttcgcgcca ttttttcggt gaagctgtca
    12001 agcaaaccgc atcccgccag ataccccagg ccaaaaaaga gcgcccccag aattagacct
    12061 acagaaaaat tgctttgcag taacggttaa taaacgtaaa tcataaacgg tccggtcaag
    12121 accaggatga aactcataca ccagatgagc ggtttcttca gaccgagttt atcctgaacg
    12181 atgccgtaga acatcataaa tagaatgctg gtaaactggt tgaccgaata aagtgtacct
    12241 aattccgtcc ctgtcaaccc tagatgtcct ttcagccaaa tagcgtataa cgaccaccac
    12301 agcgaccagg aaataaaaaa gagaaatgag taactggatg caaaacgata gtacgcattt
    12361 ctgaatggaa tattcagtgc cataattacc tacctgtcgt taaaaaattc acgtcctatt
    12421 tagagataag agcgacttcg ccgtttactt ctcactattc cagttcttgt cgacatggca
    12481 gcgctgtcat tgcccctttc gccgttactg caagcgctcc gcaacgttga gcgagatcga
    12541 taattcgtcg catttctctc tcatctgtag ataatcccgt agaggacaga cctgtgagta
    12601 acccggcaac gaacgcatct cccgcccccg tgctatcgac acaattcaca gacattccag
    12661 caaaatggtg aacttgtcct cgataacaga ccaccacccc ttctgcacct ttagtcacca
    12721 acagcatggc gatctcatac tcttttgcca gggcgcatat atcctgatcg ttctgtgttt
    12781 ttccactgat aagtcgccat tcttcttccg agagcttgac gacatccgcc agttgtagcg
    12841 cctgccgcaa acacaagcgg agcaaatgct cgtcttgcca tagatcttca cgaatattag
    12901 gatcgaagct gacaaaacct ccggcatgcc ggatcgccgt catcgcagta aatgcgctgg
    12961 tacgcgaagg ctcggcagac aacgcaattg aacagagatg taaccattcg ccatgtcgcc
    13021 agcagggcaa gtctgtcgtc tctaaaaaaa gatcggcact ggggcggacc ataaacgtaa
    13081 atgaacgttc cccttgatcg ttcagatcga caagcaccgt ggatgtccgg tgccattcat
    13141 cttgcttcag atacgtgata tcgactccct cagttagcag cattctttgc attaacgccc
    13201 caaaaggatc atcaccgacc cgacctataa atccacttat tccgcctaat ctggcgattc
    13261 ccaccgcaac gttagctggc gcgccgccag gacaaggcag taggcgcccg tctgattctg
    13321 gcaagagatc tacgaccgca tcccctaaaa cccatacttt ggctgacatt tttttccctt
    13381 aaattcatct gagttacgca tagtgataaa cctctttctc gcaaaatcgt catggattta
    13441 ctaaaacatg catattcgat cacaaaacgt catagttaac gttaacattt gtgatattca
    13501 tcgcatttat gaaagtaagg gactatattt ttataaaagt taacgttaac aattcaccaa
    13561 atttgcttaa ccaggatgat taaaatgacg caatctcgat tgcatgcggc gcaaaacgca
    13621 ctagcaaaac ttcatgagca ccggggtaac actttctatc cccattttca cctcgcgcca
    13681 cctgccggat ggatgaacga tccaaacggc ctgatctggt ttaacgatcg ttatcacgcg
    13741 ttttatcaac atcacccgat gagcgaacac tggggaccaa tgcactgggg acatgccacc
    13801 agcgacgata tgatccactg gcagcatgag cctattgcgc tagcgccagg agacgataat
    13861 gacaaagacg ggtgtttttc aggtagtgct gtcgatgaca atggtgtcct ctcacttatc
    13921 tacaccggac acgtctggct cgatggtgca ggtaatgacg atgcaattcg cgaagtacaa
    13981 tgtctggcta ccagtcggga tggtattcat ttcgagaaac agggtgtgat cctcactcca
    14041 ccagaaggaa tcatgcactt ccgcgatcct aaagtgtggc gtgaagccga cacatggtgg
    14101 atggtagtcg gggcgaaaga tccaggcaac acggggcaga tcctgcttta tcgcggcagt
    14161 tcgttgcgtg aatggacctt cgatcgcgta ctggcccacg ctgatgcggg tgaaagctat
    14221 atgtgggaat gtccggactt tttcagcctt ggcgatcagc attatctgat gttttccccg
    14281 cagggaatga atgccgaggg atacagttac cgaaatcgct ttcaaagtgg cgtaataccc
    14341 ggaatgtggt cgccaggacg actttttgca caatccgggc attttactga acttgataac
    14401 gggcatgact tttatgcacc acaaagcttt ttagcgaagg atggtcggcg tattgttatc
    14461 ggctggatgg atatgtggga atcgccaatg ccctcaaaac gtgaaggatg ggcaggctgc
    14521 atgacgctgg cgcgcgagct atcagagagc aatggcaaac ttctacaacg cccggtacac
    14581 gaagctgagt cgttacgcca gcagcatcaa tctgtctctc cccgcacaat cagcaataaa
    14641 tatgttttgc aggaaaacgc gcaagcagtt gagattcagt tgcagtgggc gctgaagaac
    14701 agtgatgccg aacattacgg attacagctc ggcactggaa tgcggctgta tattgataac
    14761 caatctgagc gacttgtttt gtggcggtat tacccacacg agaatttaga cggctaccgt
    14821 agtattcccc tcccgcagcg tgacacgctc gccctaagga tatttatcga tacatcatcc
    14881 gtggaagtat ttattaacga cggggaagcg gtgatgagta gtcgaatcta tccgcagcca
    14941 gaagaacggg aactgtcgct ttatgcctcc cacggagtgg ctgtgctgca acatggagca
    15001 ctctggctac tgggttaaca taatatcagg tggaacaacg gatcaacagc gggcaaggga
    15061 tccgcgtcac tcttccccct tcacgacctt caataatatg caatgcagct tcccgcccga
    15121 taatgtcatg tggaagctga attgtggtca gcggcggtaa aaacagatgc ccgacgccaa
    15181 ccagattatc aaagcccatt acggcgacat catgcgggat tcgtaccccc ttcgccagaa
    15241 gaacctgata agccacaaag gctgcgcgat cgttaccaca tatcagaaca tcaaaatctg
    15301 gtttgcccga tttgaagtgg tcattgagta aacttgcgag atcggtgtag tgatcatcac
    15361 ctgttgccat gtgaaattgt ttcacctcag ccagatctcg tccagcatca cgccaggcct
    15421 gctcaaatcc ctgccgacga taccctgttg ccaacgcact ttccggtagc cagaagcata
    15481 acggttgacg atagcccgcc gcgagcaaat gctgtgttga ttcatattgt gcagtgtaat
    15541 catcagggat ataactgggt aacgctgggt catccgccac acagttcgcc aatacaatat
    15601 tttcaccata cagagactca ggcagcgtga tatgtcgcag ccccattgta gtatagataa
    15661 tgccatccgg acggtgggca agcagctgac gtgccgcgcg ggcagcgtca tcttcagaaa
    15721 aaatattgat taaaaaacta ttccagccga actcgctggc ggtttgctca atggcaagca
    15781 gaatatcaac agagaaagga gtggtagccg tgtcctgcgc cagcacggcg agagtcgacg
    15841 gcttacgtcc ttgagcgcgc atcttacggg cggaaagatc aggaacataa ttcagggtct
    15901 ggattgcctg caatacgcgg tcacgcgttg caggacgcac agattctgca ttatgcatca
    15961 cccgggagac tgtcatcatc gacactcccg ccaggcgtgc tacatccttt aatgaagcca
    16021 tacccaagcc gtttgccgta aaacgggcac tgtagcagaa acagacgtca ctggcgagat
    16081 ccaacgatat tctcaacgcc ctatcacccg acacggcaat acaataaaaa ataacaataa
    16141 ttgccggata attgtactca attccgcctc tgttctcgcc aacgagtaac catataggca
    16201 agtaagtaaa acatgccaac tggcatcgcc aacctggaga agaaaatata aatatggatc
    16261 attgtgctgc cgctgtatcc cgacatcagc ccggaaatta ttgcgattgc tatcggttca
    16321 ggtgcaattg gctgcacgat cgttacggac tcgcttttct ggctagtgaa gcaatattgc
    16381 ggcgctacgc tcaatgaaac atttaaatac tatacgacag cgacatttat cgcttcagtc
    16441 gtcgctctgg cgggcacatt cctgctgtca tttatcatct aagcgcaaag agacgtacta
    16501 tggaaaacgc taaaatgaac tcgctcatcg cccagtatcc gttggtaaag gatctggttg
    16561 ctcttaaaga aaccacctgg tttaatcctg gcacgacctc attggctgaa ggtttacctt
    16621 atgttggcct gaccgaacag gatgttcagg acgcccatgc gcgcttatcc cgttttgcac
    16681 cctatctggc aaaagcattt cctgaaactg ctgccactgg ggggattatt gaatcagaac
    16741 tggttgccat tccagctatg caaaaacggc tggaaaaaga atatcagcaa ccgatcagcg
    16801 ggcaactgtt actgaaaaaa gatagccatt tgcccatttc cggctccata aaagcacgcg
    16861 gcgggattta tgaagtcctg gcacacgcag aaaaactggc tctggaagcg gggttgctga
    16921 cgcttgatga tgactacagc aaactgcttt ctccggagtt taaacagttc tttagccaat
    16981 acagcattgc tgtgggctca accggaaatc tggggttatc aatcggcatt atgagcgccc
    17041 gcattggctt taaggtgaca gttcatatgt ctgctgatgc ccgggcatgg aaaaaagcga
    17101 aactgcgcag ccatggcgtt acggtcgtgg aatatgagca agattatggt gttgccgtcg
    17161 aggaaggacg taaagcagcg cagtctgacc cgaactgttt ctttattgat gacgaaaatt
    17221 cccgcacgtt gttccttggg tattccgtcg ctggccagcg tcttaaagcg caatttgccc
    17281 agcaaggccg tatcgtcgat gctgataacc ctctgtttgt ctatctgccg tgtggtgttg
    17341 gcggtggtcc tggtggcgtc gcattcgggc ttaaactggc gtttggcgat catgttcact
    17401 gcttttttgc cgaaccaacg cactcccctt gtatgttgtt aggcgtccat acaggattac
    17461 acgatcagat ttctgttcag gatattggta tcgacaacct taccgcagcg gatggccttg
    17521 cagttggtcg cgcatcaggc tttgtcgggc gggcaatgga gcgtctgctg gatggcttct
    17581 atacccttag cgatcaaacc atgtatgaca tgcttggctg gctggcgcag gaagaaggta
    17641 ttcgtcttga accttcggca ctggcgggta tggccggacc tcagcgcgtg tgtgcatcag
    17701 taagttacca acagatgcac ggtttcagcg cagaacaact gcgtaatacc actcatctgg
    17761 tgtgggcgac gggaggtgga atggtgccgg aagaagagat gaatcaatat ctggcaaaag
    17821 gccgttaata acgtttcaac gcagcatcgc aatcctttcc ctgggtgagc gatgctgccg
    17881 atggcgcaga cttaagatcc ccggtcttac ccgctataac cccctccttt tatcatcacc
    17941 caatgccttt cgctgtaaac ggcggtttcg caaaccaaac caaaaccgta agtaagataa
    18001 aagcaatagc cgccatacgg aaaatctcat ttgcagaaat agaaagtgac tgctgggtta
    18061 tttcattatt aatttcatta agaactcctg aaagcgaacc atagtattta tccataattt
    18121 gtgacgatga attaaacacg gggttaaatt gatcgatggt tgctgtcaac tgactatggt
    18181 gtaacgattc gcgtcgtccc cacagcgtca ttgtcaacga cgtaccaact gatcctgaca
    18241 aggtacgaaa aaaattactc atactcgagg cattggcaaa tttattatct ggcaagcctg
    18301 aaaacgaaat cgttgttaag ggtaaaaaga aacaggcaac ggcgaatccc tgaaaaaact
    18361 gcggcaaaat gatgcctgta aaatcaatcg ttggcataaa tgtcacagaa cgccagtaat
    18421 agcaaaccgc atacatcaaa aaactaaatg tcactaacaa ccgcatgtct attttgttgc
    18481 cataacgtcc tatcaaaggt gaaattaata gtggcatgat gccgatgggc gcataagcaa
    18541 gtccggccca tatcgcatta taccccatcg tttcctggag taactgcggc ataaggacga
    18601 tcgctccaga gtaaaataaa tacgcgcatg tgatactcac aataccaatg gtgaagttac
    18661 gggacttaaa caaactgaga tcaagaatcg ggttctctga ggtcgactcc caaatgacta
    18721 aagagatcag agaaataact gatactactg ttaatattat tattgtactc gagttgaacc
    18781 aatccagatc gcgcccttta tcaagcataa tttgcaagcc accaacaccg agcactaaca
    18841 gggtcagtcc tggtagattc attttgaccg gtgaagtctc agtttctctt cctttaagta
    18901 aggttaagca taatgtcagg acgataatcc ccatagggac attgattaaa aatatccaac
    18961 cccagctaaa gttatcacaa atataaccgc ccaatatcgg cccacatatc ggagcgataa
    19021 tcacggtcat tgaccataat gccagagcaa atgttctttt ttctggcgga taattcctta
    19081 ataataaact ctgtgacagt ggaattaacg gccccgccat taacccctga acgactctaa
    19141 aaaatatcag cacatcaaga ttggtcgata ggctacacat taatgaagac agcgaaaaaa
    19201 aggttactga aagtaaaaat aatcttaatt cgcctattct ttgtgccaac ctgccagtaa
    19261 cagggatcgc aatggcattt gctacaccaa acgaggtgat aacccaggtg ccttcgtctg
    19321 ttgatgctcc cagaaagcca gatattgtcg gtattgcgac gttagaaata gtggaatcca
    19381 acatttgcat aaatgtcgct aatgacaatg caatagtgac gcaccataac gtcccaccgg
    19441 ttaatggtgc cggagttgat ttagtgattg ccattaaaat tgtccattat gcgaaataat
    19501 gttgctaatt tctttttcta tcggactggt atcgataact aaagccttac tggtataagc
    19561 cggcatggag gtcacggttg aagccagctc aggcatctcg gcaatgtctt cgttcttcgt
    19621 atcaatagtt gctgtcatcg ataaaccaat acgcaaggga tgttccatga gttcttttgg
    19681 atcaagagaa acttcaaccg gtacacgctg aacgattttg atccagttcc ctgtcgcatt
    19741 ttgtgcaggt aataaggaga acgcattgcc ggttcccata ttgatccctg tcacccgacc
    19801 atgaaacaca acattttcac cataaagatc gctgataata ttgaccgatt gaccaatccg
    19861 tacatccgtg agttgtgttt ctttaaagtt ggcattaacc cacatttgac gtgccggtac
    19921 gacagccatt aacgattgtc cggggctcac tgtttcgccg acctgaacac ttcgctgggc
    19981 aatatagccg gtaaccggac tcttaatatc cgtacgttta agcgccaacc aggcttcttt
    20041 agttgcatcc gccgcttcaa cgacttgtgg ctgacggttt aatggtgtgt tcattactaa
    20101 agctttattc gctttataag cctggatagc ggcattcaat gccgctttgc tacttattaa
    20161 cgtatctttg gtatgctcca gcgtttcttt tgaaataacc ccctgcttcg ctaacggcac
    20221 tcgacggtta taatcttcta aagattgttg atactgaata cgtgctgaag cgacttcggc
    20281 actgtattgt ttatcctgta agtatagttt attcgtttgc cgaacaatat ttgccagatt
    20341 atttttagct ttattgagtg cgatagtggc atcagtttta tccagtgaaa ctaaaatgtc
    20401 accttgtcga acgtagttcg tatctttatg attaacgaca gtgacactac ctgagacttg
    20461 tgcagaaatt ggatctgcat tccccgtgac ataggcgtca tctgtactaa tcatgtcttc
    20521 taattccatt gaccaatagg cataggcacc tgaaaacgca ataaataaaa ctaccgccaa
    20581 taaagaaaag tattttcttc tgttagaatg ttttttattt gaattaatct gttccactat
    20641 tatctctcat ttctcataga tgaaatttat gaattgaatg aaaagagcat tcattcttca
    20701 tatcaaacac agtgcatgtg ttgcacatta tcccaggaac gttggaggcc taatttaaat
    20761 gaggtgcaaa aaaaacaaca gaggactaaa ccgtggcttt tgcaatacaa ttcttacgcc
    20821 tgtaggatta gtaagaagac ttatagtgct aacttgaaac tataaatcac cggtacaatc
    20881 cctgatttta ttgttgacat ttcatttatg ccgactattt atatggtata cttgtcgaat
    20941 tatcttaaag gaagctcaga ttttcttatt tttattgaga aaatgagatg acgccttatg
    21001 tctgtattac tacagggaga agggagatgc ttcattgcaa agggaataat ctatgaacgc
    21061 aataattatt gatgaccatc ctcttgctat cgcagcaatt cgtaatttat tgatcaaaaa
    21121 cgatattgaa atcttagcgg agttgactga agggggaagc gccgttcagc gggtggaaac
    21181 acttaagcct gatatcgtca tcattgatgt cgatatcccc ggagttaacg gtatccaggt
    21241 gttagaaacg ctgaggaagc gccaatatag cggaattatt attattgtct ccgctaaaaa
    21301 tgaccatttt tacgggaaac attgtgctga tgctggcgct aatggattcg tgagtaaaaa
    21361 agaaggcatg aacaatatca ttgcggctat tgaagctgca aaaaatggct actgctattt
    21421 ccccttctct ctcaaccggt ttgttggaag tttaacgtcc gaccagcaaa aactcgactc
    21481 cttatcgaaa caagaaatta gtgtcatgcg gtatattctt gatggcaagg ataataatga
    21541 cattgctgaa aaaatgttca tcagcaacaa aactgtcagc acttataaaa gtcgcctgat
    21601 ggaaaaatta gaatgtaaat cactgatgga tctttacaca ttcgcgcaac gtaacaaaat
    21661 cggctaatca catgaagttt ttaccctatt tttttcttct ctgttgtggt ctttggtcga
    21721 ccataagttt cgcagacgaa gattacatcg aatatcgtgg catcagtagt aacaaccgtg
    21781 tcacacttga tccagtacgt ctgagcaata aggaattacg ttggttagcg agcaaaaaaa
    21841 atcttgtgat tgcagtacac aagtcccaaa cggctacctt gttgcatacc gattcgcagc
    21901 aacaggttcg tggtattaat gctgattatt taaatctttt aaaaagagcg ttaaatatca
    21961 aattaacact ccgggaatac gcagatcatc aaaaagcaat ggacgcgctt gcagaaggtg
    22021 aagtcgatat agtgttatca catttagtta cttcgccgcc tcttaataat gacattgctg
    22081 caaccaaacc attgataatt acctttccgg cgctggtaac cacccttcac gactcaatgc
    22141 gaccgcttac ctcaccaaaa ccagtaaata ttgctcgggt agcaaattac cccccagacg
    22201 aggtaattca tcaatcattt ccaaaagcaa caattatctc ttttacaaat ttatatcagg
    22261 cattagcatc cgtctcagct gggcacaatg attactttat tggtagtaac atcattacca
    22321 gcagtatgat ttcccgctat ttcactcact ccttaaatgt agtgaaatat tataactcgc
    22381 cgcgtcaata taattttttc ttgaccagaa aagaatctgt cattcttaat gaagtactca
    22441 atagatttgt tgatgcttta acaaatgaag ttcgctatga agtatcacaa aattggcttg
    22501 atacaggaaa cctggccttt ctgaacaaac cattagaact cactgaacat gaaaaacagt
    22561 ggattaagca gcatcccaat ttaaaggtgc tggaaaatcc ttactcgccc ccctattcta
    22621 tgacggatga aaatggctcg gttcggggcg ttatggggga cattcttaat attattacct
    22681 tgcaaacagg tttaaatttt tctccgatca ccgtttcaca caatatccat gctggaacac
    22741 agcttagccc cggaggatgg gatataatac ctggcgctat ttatagtgaa gatcgagaaa
    22801 ataatgtttt atttgctgaa gccttcataa caacgcctta cgtttttgtc atgcaaaaag
    22861 cgcctgacag tgaacaaaca ttaaaaaaag gaatgaaagt tgccattcca tattattatg
    22921 agctgcattc gcaattaaaa gagatgtatc cggaggttga atggatacag gtcgataatg
    22981 ccagcgctgc atttcacaag gttaaggaag gtgaacttga tgctctggtc gcgacacagt
    23041 taaattcgcg ttacatgatc gatcattact atcctaatga actttatcat tttcttattc
    23101 ctggcgttcc gaatgcatcg ctttcgttcg cttttcctcg cggagaaccg gaacttaagg
    23161 atattattaa taaagcactg aatgcaattc ccccaagcga agttctgcgc ctgacggaaa
    23221 aatggattaa aatgcccaat gtgaccattg acacatggga cctatatagc gagcaatttt
    23281 atattgttac gacattatcc gttttattag ttggcagtag ccttttatgg ggattctacc
    23341 tgttacgctc agttcgtcgt cgtaaagtca ttcagggtga tttagaaaac caaatatcat
    23401 tccgaaaagc actctcggat tccttaccga atccaactta tgttgtaaac tggcaaggta
    23461 atgtcattag tcataatagt gcttttgaac attatttcac tgcggattac tacaaaaatg
    23521 caatgttacc attagaaaac agtgactcac cctttaaaga tgttttttct aatgcgcatg
    23581 aagtcacagc agaaacgaaa gaaaatcgaa caatatacac acaggtattt gaaattgata
    23641 atggcatcga gaaaagatgc attaatcact ggcatacatt atgcaatctt cctgcaagtg
    23701 acaatgcagt atatatttgt ggttggcaag atattactga aacgcgtgat ctaattaatg
    23761 cactcgaggt agaaaaaaat aaagcgataa aggctaccgt agcaaaaagt cagtttctgg
    23821 caacgatgag tcacgaaata agaacaccaa taagctctat tatgggcttc ctggaacttc
    23881 tgtcgggttc tggtcttagc aaggagcaac gggtggaggc gatttcactt gcctacgcca
    23941 ccggacaatc actcctcggc ttaattggtg aaatccttga tgtcgacaaa attgaatcgg
    24001 gtaactatca acttcaacca caatgggtcg atatccctac tttagtccag aacacttgtc
    24061 actctttcgg tgcgattgct gcaagcaaat ccatcgcatt aagttgcagc agtacatttc
    24121 ctgatcatta cctggttaag atcgatcctc aggcgtttaa gcaggtctta tcaaatttac
    24181 tgagcaatgc tctcaaattt accaccgagg gggcagtaaa aattacgacc tccctagttc
    24241 acattgatga caaccacgct gttatcaaaa tgacgattat ggattctgga agtggattat
    24301 cgcaggaaga acaacaacaa ctctttaaac gctacagcca aacaagtgca ggtcgccagc
    24361 aaacaggatc tggcttaggc ttaatgatct gcaaagaatt aattaaaaat atgcagggcg
    24421 atttgtcatt agaaagtcat ccaggcatag gaacaacatt tacgatcaca atcccggtag
    24481 aaattagcca gcaagtggcg actgtcgagg caaaagcaga acaccccatc acactacctg
    24541 aaaagttgag catattaatc gcggatgatc atccgaccaa caggctatta ctcaaacgcc
    24601 agctaaatct attaggatat gatgttgatg aagccactga tggtgtgcaa gcgctacaca
    24661 aagtcagtat gcaacattat gatctgctta ttactgacgt taatatgccg aatatggatg
    24721 gttttgagtt gactcgcaaa ctccgtgagc aaaattcttc cttacccatc tgggggctta
    24781 cagccaacgc acaggctaac gaacgtgaaa aagggttaag ttgcggcatg aacttatgtt
    24841 tgttcaaacc gttgaccctg gatgtactga aaacacattt aagtcagtta caccaggttg
    24901 cgcatattgc acctcagtat cgccaccttg atatcgaagc cctgaaaaat aatacggcga
    24961 acgatctaca actgatgcag gagattctca tgactttcca gcatgaaacg cataaagatc
    25021 tacccgctgc gtttcaagca ctagaagctg gcgataacag aactttccat cagtgtattc
    25081 atcgcatcca cggtgcggct aacatcctga atttgcaaaa gttgattaat attagccatc
    25141 agttagaaat aacacctgtt tcagatgaca gtaagcctga aattcttcag ttgctgaact
    25201 ctgtaaaaga acacattgca gagctggacc aggagattgc tgttttctgt cagaaaaatg
    25261 actaaatagc ggctcccaca atgttcaaat gtgggagcta tttaccagca catcttacat
    25321 ttatgatgag aactcctggc gaatttgttc cccatgctgg tcgagcattg ccgctcccgg
    25381 cataacatgc gggtccgcgc agccgctgat ttttatcgga ttacccggca tcattattcc
    25441 cccggcttca atcaacatat ttcttgcctg agtttgtggc aatttaatgg cctcagccac
    25501 acttaataac ggcgcgacgg gtacaccaac ttcatgtatt ctggctaacc aaacttcagc
    25561 tgcctgcgtt tttaacgtcc gctcaatata ttgtttaaga atagcctggt tttgtacgcg
    25621 taaaatattg ctgctaaatc ggggatcatt aaccagttcc gtaagctcca gtgcctggca
    25681 taacgcagaa aaaagcttgt cattaccaca acaaatcgta atcggcttat cctgagtatt
    25741 gaaaacatca aaaggtgcca tgtagggatg gcgatttccc agacgttgtg gtgacttccc
    25801 tgtcgcgata tatgccatca gaccatgctc cagaaaactc agcgtggcat caaacatcgc
    25861 tatatcgaca tgcgcccctc tctggctctt ttcgcggcca taaagtgcac tcactattcc
    25921 gctgaataaa tagacaccgc cgcatagatc cgcaagagat gtaccaacgc gcactggcgg
    25981 agcatcagga tatcctgttt ccatcattat cccgctcatt gcctgaatga tggtatcgta
    26041 ggcaggagca tcttttagcg gaccggtatg tccgaaacct gacgatgaag catatatgag
    26101 gcgcgggttg atttcttgaa gcgtttccca tgaaaacccc agtttttcca ttgtacctgg
    26161 gcgaaaattc tcagctaata catcagcttg tttgagcata tttataaata tacttttatc
    26221 gtgatcattc tttaaatcaa gaaccacact ctctttgcca tgattaataa aactgtaata
    26281 gagtgactgt ccatccacat agggaccaaa tgtgcgggta tcatcaccat gacccggcgg
    26341 ctcaacttta attacccttg cgcccatatt acaaagaagt tgagttccga aaggtccatt
    26401 aaggacatgt gtcatatcga taactaataa gccttcaaac ggccctttgc tttcattatt
    26461 tgtcatttgc ttatgccttg aaaaaagccg aaggtgaaaa gacacttcgg catttatgta
    26521 aaatcaataa catgattaat tatgaaacca gtcgactgac ataaatccac aagggagccg
    26581 tgacgacaaa gcccagtacg ctcacagcca atgacgctgt accagtacgg gtgtagacat
    26641 taaaccggct ggcaataatg attccggaga atgccggcgg taatgcgcct gccagtacca
    26701 tcatctgcag atgttcgctg ttcaaatgac atgccatacc aacgagaagc agtgccagtg
    26761 gcatcagaat cagcttcagg aaggtgttat aagcaatttc ggcactgaac tcgaatttat
    26821 gtgcagccag agtcaacccc gcagcgaata ccgctacccc tgagttagcc ttcgcaatca
    26881 gattaaaggt tgggtcccat gctgccggaa tttttacccc aaccaacacc aggatcgttg
    26941 ccagaacagg tgcccatact actggctcct ttgccgcaga aattaatgcg ctcagattac
    27001 tattcttctt accatccgct cctgaggaag gattcagcaa atacagacca ataggaatag
    27061 taattgcgtt aacaataata gaaataattg ctaccactaa acctgttgat acggaatcac
    27121 cataaatagg atcgagaact gcaaacccca ggaatccaat ggtaggtgaa cccgcaatta
    27181 atgcacatac agctgcttct gcatgggtac gtttaaaaaa tttgtagcaa ccaaaccagg
    27241 agaaaaagaa acatccgaca ataaccacaa gtgatacaag ggtcagacga gtgtccgcaa
    27301 aaatcatttc cctgtttgcc cgggtaatag aaacaaatag agccgcagga agagcgtagt
    27361 ttaataccag tttattaaat gcgcgggctt gatcttctga aaaagtttct cgtctgccgc
    27421 taaaataacc caacagcatg attacgataa taggcaataa atcgccaata aaaaatgtta
    27481 gcataatagt atttccgata agggtcccct caccatcgca taatgagttg gcaagaggag
    27541 acgatgtcag tatttaatta ccagcgactt gttttgggtt aagtttggta atatggccac
    27601 tttcagttcc tgctgcaggg tcgatgacca cattaataat ggtcggtttg cgcgactgaa
    27661 taccggtggt taaagcatga cgaagttcat ctgtcgtggt gacgttatag ccaacgccac
    27721 gaaacgcatc cattaatttg tcataccttg catggtgcaa cagatccgtt ggtgatggtg
    27781 caccagcgcc actgagatca acaccgtctc ctctgtagat gccgccatta ttaaaaataa
    27841 cgatcgtcac cggcaggtta tatcgacaaa tcgtttcaat ttccatccca ctgaaaccaa
    27901 aagcactatc accttcaatg gcgacaaccg gagaaccaga agtcacgcta gcaccgatgg
    27961 catagcccat accgatgccc atgacacccc aggtaccaca atccagacga cgacgtggtt
    28021 tatacatatc aataatattt cgtgcattat ccagggtatt tgcaccttca ttaactaaat
    28081 aaatatcctg gttctcgcgc aatacgtcgc gcacagcact taatgcatta aagtaattta
    28141 atggttgagt atctgtactt aatttttcat gcattttttg tgcattttgc tgcttgtgga
    28201 tatttaaaat atcgcgccat accagtggag tcgtaaatgt gttttgtttc agttctgcca
    28261 gcataccttg catactggat gcaatatcac caacgactgg cacagcaatg gggcggttgc
    28321 tgtcaatttc ctgcggttca atatccagtt gaataaactg tgtatctgcc gcccatcctt
    28381 ttttaccgtg tgccaataac caattcagtc gtgcaccaac aagcatgaca acgtcagcat
    28441 ttgccagggc aaacgaacgc gcagctgccg cagaaagtgg atgcgtatct tcaaggatcc
    28501 ctttcgccat agacattggc aggaatggaa tctgagtact ttcaataaat tcacgaagct
    28561 gttcatcagc ttgtgaatac gccgcgcctt tgccaaggat aattaatggc cgttcagctt
    28621 ttgctaaaag cgaaattgcg ctagtgactg acttcgggca tggcaataat gctggcgacg
    28681 gattttcaac tttaacaatc gtggttaacg cttcgtcttt ttccatcgtc gcggccagga
    28741 catttgctgg caaatcaaga taaactccgc cagggcgacc cgatacagag acccggatag
    28801 cgcgtgccaa tgcaatgcca agatcctgcg gctgattaac gcgaaatgct gctttggcat
    28861 acggttttgc cgcattcatt tggtccagct cttcataatc accttgctgt aggtcgacga
    28921 tcgcgcggtc gctggagccg ctaatcatga tcatcggaaa accatttacc gttgcgttgg
    28981 ccaatgcggt caaaccattg aggaatcctg gcgcagaaac tgtcaggcag atccccggtt
    29041 tttgggtaag aaaaccgctt gccgcagcgg catagcctgc cgactgctca tgacgaaaac
    29101 caatataacg aatgccttcc gcctgggcat ggcgtgccat atccgtcaca ggaataccta
    29161 caacaccata aatagtgtca atattattct gttttaatgc ttcaacgatg atatgcatac
    29221 catctgtcat ttgaagttga tctgacatga ttaataacct cgaaattttt ataaattggg
    29281 agccagaagc ccccgttaat atcagatggc gtggttttgc ttcattgcag caatttcatc
    29341 gtcactataa cccagctcct gcaatacagc agcggtatgt tcacctaata gcggcgcagc
    29401 tttaatatcc ggcgtaaagg cagagaattt cattggacaa ccaacggtca gatattttcc
    29461 acgcaacggt tgttccactt caacaacact gccactttgg cgcaaagagg gatcaagtga
    29521 aatttctttc atacttaaaa ccggtgcaca aggaatatca aactgagtca aataggccac
    29581 cgcttcatgt ttatcaatag tgacagtgta tttttcgatt tcagcaaaaa tatcgaaaat
    29641 atgtggctgt cgtgcatggg ctgtactgta tgccggatcg gtaatccatt ctggtttgcc
    29701 gatggcttta caggtgtttt cccagttttg ctcctgaata gtgaaataaa tataggcgtt
    29761 aggatcggtt tcccagcctt tacatttcag gatccagcca ggctgaccgc caccacctgc
    29821 attaccaccg cggggaactg catcaccaaa tgtaccattc ggatactgcg ggtattcttc
    29881 cagataaccc aatttatcga gacgctgctg gtcacgtaat ttcacgcggc aaaggttcaa
    29941 tacggcatcc tgcattgaca tggtgactcg ttgcccacgc cccgtttttt cgcgatgcag
    30001 caaagcagca agtaaaccga tcagcaaatg cattccggtg ttgctgtcac ccaacgctgc
    30061 agcgcttacc agcggcggac catcccaaaa acccgtagtg gatgccgcgc cacccgctgc
    30121 ctgagcaacg ttttcatagg cttttacatt cacataaggc gaacactcat caaacccttt
    30181 gatcgaacca aaaatcagac gtggattgat ttcttgaata tgctcccagg tgaagcccat
    30241 gtgatcaatg gcccctggat gaaagttctc gactaagata tcagcttcgc ggatcagctt
    30301 ttccattacc tctttgcctt ccgctgtttt ggtatttaac tcaatagaac gtttgttact
    30361 gttaagcatg gtgaagtaaa gcgcatcgat atcaggaata tctcgcagct ggtgacgcgt
    30421 tacgtcacca acgccgggac gttcaatttt aataacgtca
//
LOCUS       DAFNBJ010000049        29750 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000049 DAFNBJ010000000
VERSION     DAFNBJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.049"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="L2X49_002026"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="L2X49_002026"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBN4797112.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTAPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="L2X49_002027"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="L2X49_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBN4797113.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="L2X49_002028"
     CDS             complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="L2X49_002028"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBN4797114.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="L2X49_002029"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="L2X49_002029"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBN4797115.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="L2X49_002030"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="L2X49_002030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBN4797116.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="L2X49_002031"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="L2X49_002031"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBN4797117.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="L2X49_002032"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="L2X49_002032"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBN4797118.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="L2X49_002033"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="L2X49_002033"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBN4797119.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="L2X49_002034"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="L2X49_002034"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBN4797120.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="L2X49_002035"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="L2X49_002035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBN4797121.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFMMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="L2X49_002036"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="L2X49_002036"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBN4797122.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLQAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="L2X49_002037"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="L2X49_002037"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBN4797123.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="L2X49_002038"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="L2X49_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBN4797124.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="L2X49_002039"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="L2X49_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBN4797125.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKDSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="L2X49_002040"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="L2X49_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBN4797126.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="L2X49_002041"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="L2X49_002041"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBN4797127.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKVLPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="L2X49_002042"
     CDS             complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="L2X49_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBN4797128.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="L2X49_002043"
     CDS             complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="L2X49_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBN4797129.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFCGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19506..21374
                     /gene="kup"
                     /locus_tag="L2X49_002044"
     CDS             19506..21374
                     /gene="kup"
                     /locus_tag="L2X49_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBN4797130.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21541..21960
                     /gene="rbsD"
                     /locus_tag="L2X49_002045"
     CDS             21541..21960
                     /gene="rbsD"
                     /locus_tag="L2X49_002045"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBN4797131.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPISKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            21968..23473
                     /gene="rbsA"
                     /locus_tag="L2X49_002046"
     CDS             21968..23473
                     /gene="rbsA"
                     /locus_tag="L2X49_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBN4797132.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLGKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23478..24443
                     /gene="rbsC"
                     /locus_tag="L2X49_002047"
     CDS             23478..24443
                     /gene="rbsC"
                     /locus_tag="L2X49_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBN4797133.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24468..25358
                     /gene="rbsB"
                     /locus_tag="L2X49_002048"
     CDS             24468..25358
                     /gene="rbsB"
                     /locus_tag="L2X49_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HBN4797134.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25484..26413
                     /gene="rbsK"
                     /locus_tag="L2X49_002049"
     CDS             25484..26413
                     /gene="rbsK"
                     /locus_tag="L2X49_002049"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBN4797135.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26417..27409
                     /gene="rbsR"
                     /locus_tag="L2X49_002050"
     CDS             26417..27409
                     /gene="rbsR"
                     /locus_tag="L2X49_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBN4797136.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLSIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27375..28802)
                     /gene="mdtD"
                     /locus_tag="L2X49_002051"
     CDS             complement(27375..28802)
                     /gene="mdtD"
                     /locus_tag="L2X49_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBN4797137.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28825..29517)
                     /locus_tag="L2X49_002052"
     CDS             complement(28825..29517)
                     /locus_tag="L2X49_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBN4797138.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7153 a         7635 c         7605 g         7357 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggagcggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca actgaccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc aacagttcgt tgttcggtgc
      301 aacgacgata accggcatat ccgcgtcaat cagcgccagc gggccgtgtt tcagctcgcc
      361 tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc agcttcaatg cgccttccag
      421 cgcgattggg tactgatcgc cacggcccag gaacagcgcg tgatgtttgt cagagaaatc
      481 ttctgccagc gcttcaatgc gtttgtcctg agacagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggatgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttgc gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtaacggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttga tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtaattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccac gcagttgcgg gatagcacgc agaacagcct cacgcagagt
     1441 accgccctgt ttcagctccc agttcaccag atgggcaatt acttcggtgt ctgtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcgcgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcattca cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtatgagc aatacccgta ccgccatgca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgacca gctttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa caaagttacc gacgtgtgca ccttccagca actcagcacc aggacgcaga
     2401 cgggcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaggtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gcgcagcacg ctccagcaga cgagagtgga ggtagaatac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggagccaga tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttaccgg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc aattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc aagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccaat
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 atgaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgcctgt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 gcgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttctgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactggtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat gcaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cctggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggttca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 ccaggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc
    12001 gtgacgccag aaatacgcga agcctggccg atagaggctg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaggtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatac
    12601 gcctgagaac gcgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccggccagta aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atagagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcgt tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtcg
    12961 gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcat acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
    13201 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagaaagcgg aatggacggc ggatcaccag cacggccacc gctgtaatta
    13321 tccagaccga tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcttgg
    13921 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactgccc tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aaccgttgtt ccttggataa ctaccggttg atccaagctt
    14161 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatctttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgatcaggg cttgatagta cgcatgatcg
    14761 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat tacttttata aagatgctgt
    14881 agtggcctgt tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctacccg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    15241 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtgtgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagttctg cctgatgccc
    15541 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc
    15841 acttcgtaca cagccaggag ttactgtctc gttatccgga tcttgatgcc aaagggcgtg
    15901 agcgggcgat agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga
    15961 gcgatggtca tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggtca tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgttgaag catcagttgg
    16141 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgactatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gagtatggcc agctgctgtt cgcgagagcg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gccacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt tttctgcgag
    16741 agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca ggagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt
    17221 aagctgaccg gcgctcatat cccacagacg accagctgca gtattgttat ctgcgagaat
    17281 cggttcaagt tgtccgctca gcgtcatgcg ttcctgaact tcactcaaca gttgttcgcg
    17341 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    17401 acgctggaga aaaagcgtgt gtaacgctga agtgatcgtg ctgttagcat caaccaactg
    17461 tcgggcttgt tctgcccatg gagaattcag acgatgcagt aagtccagga tctgtggtag
    17521 ctgcacaata aactgtggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    17581 ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgctt cacgccagcg
    17641 gggaacatca tcagtgattg ccgccttcag gcgtgggaat ttttcaaaga agaccgccag
    17701 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    17761 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcaccg
    17821 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    17881 tgctgttcgc tgaataaagc gtgttgcttg cgccagtcgc tttcaagctc ttccagttgt
    17941 tgttttattt caccaggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    18001 tgtaaactta catcgcgaac gacaagatgt tgggcgctat caacttccag attcagtttc
    18061 tgggcaaagc cgataccgtt cagtttaccg cgaatttcac cacctttgct cagccactgt
    18121 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    18181 ttttgcagca gtagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga
    18241 cgacggctga aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc
    18301 tgctggagtt gcaggtgacg ttgcacaatc gcgccaaggc gagtcaacat cccttgctgt
    18361 tgccaggcgt gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    18421 gcgtcatacc acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga
    18481 ccgcaaaaaa aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag
    18541 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
    18601 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    18661 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    18721 gagcggaaat tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca
    18781 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga
    18841 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
    18901 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct
    18961 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
    19021 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac
    19081 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
    19141 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
    19201 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
    19261 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
    19321 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg gggtattttt
    19381 ctttgagcca ggttaatgtg gccgcattta ggagtacgat tttgccgtta atcgtgcata
    19441 ctgtgcgctt ttttgtgggc caagggacta agcacacatt tcatatttca acgaaagact
    19501 agtctatgag cactgataat aagcaatcat tgcccgcgat taccctcgcg gcgattggag
    19561 ttgtctacgg cgatattggt accagcccgt tatatacact tcgtgaatgt ttgtccggcc
    19621 agtttggttt tggcgttgaa cgcgatgccg tgtttggctt tttatcgctg atcttctggc
    19681 tgctaatctt tgtggtttcc attaaatatc tcaccttcgt gatgcgggca gataacgccg
    19741 gtgagggggg gatcctgacg ttgatgtcgc ttgccgggcg taatacgtcg gcgcgaacca
    19801 catcaatgct ggtgattatg gggctaatcg gcggcagctt tttctatggt gaagtcgtca
    19861 taacacccgc tatttcggtg atgtcagcca ttgaaggtct ggaaatcgtc gccccgcagc
    19921 tggatacctg gatagttccc ctctcaatta tcgttctcac tttattattt atgattcaaa
    19981 aacatggcac ggctatggtc ggtaagctgt ttgcgccgat catgctgacc tggtttttga
    20041 ttctggcagg actggggtta cgtagcatta ttgctaaccc ggaagtgctg catgcgctga
    20101 atccaatgtg ggcggtgcat ttcttccttg aatacaaaac ggtttctttt attgcattag
    20161 gggcagtggt gttgtcgatt acgggggtcg aggcgctgta tgctgatatg gggcactttg
    20221 gtaagttccc tattcgcctg gcgtggttca ccgtcgtatt accttccttg acccttaatt
    20281 acttcggtca gggagcgctg ttgttaaaga acccggaagc gattaagaac ccgttcttcc
    20341 tgttggcacc ggactgggcg ctgatcccgc tgctgatcat cgccgcactg gcgacggtaa
    20401 ttgcctcgca ggcggttatc tctggcgtct tctcattgac gcgtcaggcg gtacgtctgg
    20461 gatatttatc gccgatgcgc attattcaca cctccgaaat ggagtcaggg caaatctata
    20521 ttccgtttgt gaactggatg ctctatgtcg cggtcgtgat tgtgattgtc agctttgagc
    20581 actccagcaa cctggcggcg gcgtacggga ttgcggtgac cggaaccatg gtgctgacgt
    20641 ctattctctc gactaccgtg gcacgtcaga actggcactg gaataagtat tttgttgcgc
    20701 tgatcctgat tgctttcctt tgtgtcgata ttccattgtt caccgctaac ctcgataaac
    20761 tgctctccgg cggctggttg ccattgagcc tcggtacggt gatgtttatc gtgatgacca
    20821 cctggaagag cgagcgtttc cgcttgctgc ggcggatgca tgaacatggt aactctctgg
    20881 aagcgatgat tgcttcgctg gagaaatcac cgcctgttcg cgtgcccggg accgcggtgt
    20941 atatgtcgcg tgcaatcaac gtcattccct ttgcgctgat gcataacctt aaacataaca
    21001 aggtattgca tgagcgggtg attctgttaa ctctgcgtac cgaagacgcg ccatatgtcc
    21061 ataatgtccg tcgggtacag attgaacaac tgtcgcccac tttctggcgc gtggtggcaa
    21121 gttatggttg gcgagaaacg ccaaacgtgg aagaagtttt ccaccgctgc ggtctggaag
    21181 gattaagttg ccggatgatg gaaacctcct tctttatgtc gcatgagtcg ttgatcctcg
    21241 gcaaacgccc gtggtatttg cgtctgcgcg gcaagctgta cttgctgctg caacgtaatg
    21301 cgctgcgtgc accagatcaa tttgaaatcc cgccaaacag ggttatcgaa ctgggtactc
    21361 aggtcgaaat ctaacgccag acgcctcctt tctccataag ggggcgtttt tgttttcatg
    21421 gttaatcacc atgtaaaacg tttcgaggtt gatcacattt ccgtaacgtc acgatggttt
    21481 tcccaactca gtgaggatta aactgtgggt cagcgaaacg tttcgctgat ggagaaaaaa
    21541 atgaaaaaag gcaccgttct taattctgat atttcatcgg tgatctcccg tctgggacat
    21601 accgatacgc tggtggtgtg tgatgctggt ttacccatct ccaaaagtac aacgcgtatc
    21661 gatatggcat taacccaggg tgtaccttct tttatgcagg tgctgggcgt cgttacaaat
    21721 gaaatgcagg tcgaggctgc cattatcgcg gaagagatca aacaacataa tccgcaactc
    21781 cacgaaacgt tgctcactca ccttgagcag ctgcaaaaac accagggaaa taccattgaa
    21841 attcgttaca ccacgcatga acaattcaaa caacaaaccg cagaaagtca ggcggtaatt
    21901 cgcagcggag aatgttctcc gtatgcgaat atcattctct gtgctggcgt gacgttctga
    21961 ggccgtcatg gaagcattac ttcagcttaa aggcatcgat aaagccttcc cgggcgtaaa
    22021 agccctctcg ggcgcagcgt taaatgtcta tccgggccgc gtgatggcgc tggtgggcga
    22081 aaacggcgcg ggtaaatcca ccatgatgaa agtgcttact ggtatctatg ctcgcgatgc
    22141 cggcacgctt ttatggctgg ggaaagaaac gacatttacc gggccgaaat cttcccagga
    22201 agccgggatt gggattatcc atcaggaact gaacctgatc ccgcagttga ccattgccga
    22261 aaacattttc ctcggtcgtg agtttgttaa tcgctttggc aaaattgact ggaaaaccat
    22321 gtatgccgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagct
    22381 ggtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
    22441 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
    22501 atccctgttc cgcgtcatcc gcgaactaaa atcgcaaggc cgcggtattg tctatatctc
    22561 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgacgggca
    22621 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
    22681 gggtcgcaag ctggaagatc aatatccgca cctgggcaaa gcgccgggag atatccgcct
    22741 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
    22801 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
    22861 ctacggcgca ctaccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
    22921 ccgttcaccg caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
    22981 tgacggttta gtgttgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
    23041 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
    23101 cattcgtctg tttaatgtga aaacaccgtc gatggaacag gcaattggtc tgctttccgg
    23161 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtgttgat
    23221 ccttgatgaa cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
    23281 taaccagttc aaagccgatg gcttgagcat cattctggtg tcatcggaga tgccagaagt
    23341 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac
    23401 tcgtgagcag gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt
    23461 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
    23521 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
    23581 cgttaagccc gaactttttc accatcaata acttattcaa tattcttcag caaacctcgg
    23641 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggt atcgacttgt
    23701 cggtaggttc tctgttggcg ctgaccggcg cagttgctgc atctatcgtc ggcattgaag
    23761 tcaatgcgct ggtggctgtc gctgccgctc tcgcgttagg tgccgcaatt ggtgcggtaa
    23821 ccggggtgat tgtagcgaaa ggtcgcgttc aggcgtttat cgctacgctg gttatgatgc
    23881 ttttactgcg cggtgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
    23941 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
    24001 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
    24061 tggggcgtta catctacgcg ctgggcggca acgaagcagc aacgcgtctt tctggtatca
    24121 acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg
    24181 ggatcattga agtggcgcgt ctctcctccg cacaacccac ggcggggact ggctatgagc
    24241 tggatgctat tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca
    24301 ttgttgggac gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt
    24361 taggtgtttc ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc
    24421 tggtagacaa caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa
    24481 aactggctac cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg
    24541 caaaagacac catcgcactg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga
    24601 aagatggcgc gcagaaagag gcggataagc ttggctataa cctggtggtg ctggactccc
    24661 agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa
    24721 ttctgctgat taacccgacc gactccgacg cagtaggtaa tgctgtgaag atggctaacc
    24781 aggcgaacat cccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc
    24841 acattgcttc tgataacgta ctgggtggca aaatcgccgg tgattacatc gcgaagaaag
    24901 cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc tggtacatcc gcagcccgtg
    24961 aacgtggcga aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc
    25021 agccagcaga ttttgatcgc actaaaggtt tgaacgtaat gcagaacctg ttgaccgctc
    25081 atccggatgt tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcgcg
    25141 cactgcaaac tgccggtaaa tcggatgtga tggtcgtcgg atttgacggt acaccggatg
    25201 gcgaaaaagc ggtgaatgat ggcaaactag cagcgactat cgctcagcta cccgatcaga
    25261 ttggcgcgaa aggcgtcgaa accgcagata aagtgctgaa aggcgagaaa gttcaggcta
    25321 agtatccggt tgatctgaaa ctggttgtta agcagtagtt ttaatcaggt tgtatgacct
    25381 gatggtgaca taaatacgtc atcgacagat gaacgtgtaa tataaagaaa agcagggcac
    25441 gcgccaccct aacacggtgg cgcattttat ggacatcccg aatatgcaaa acgcaggcag
    25501 cctcgttgtt cttggcagca ttaatgctga ccacattctt aatcttcaat cttttcctac
    25561 tccaggcgaa accgtaaccg gtaaccacta tcaggttgca tttggcggca aaggcgcgaa
    25621 tcaggctgtg gctgctgggc gtagcggtgc gaatatcgcg tttattgcct gtacgggtga
    25681 tgacagcatt ggtgagagcg ttcgccagca gctcgccact gataacattg atattactcc
    25741 ggtcagcgtg atcaaaggcg aatcaacagg tgtggcgctg atttttgtta atggcgaagg
    25801 tgagaatgtc atcggtattc atgccggcgc taatgctgcc ctttccccgg cgctggtgga
    25861 agcgcaacgt gagcgtattg ccaacgcgtc agcattatta atgcagctgg aatcaccact
    25921 cgaaagtgtg atggcagcgg cgaaaatcgc ccatcaaaat aagactatcg ttgcgcttaa
    25981 cccggctccg gctcgcgaac ttcctgacga actgctggcg ctggtggaca ttattacgcc
    26041 aaacgaaacg gaagcagaaa agctcaccgg tattcgtgtt gaaaatgatg aagatgcagc
    26101 gaaggcggcg caggtactgc atgaaaaagg tatccgtact gtactgatta ctttaggaag
    26161 tcgtggtgta tgggctagcg tgaatggtga aggtcagcgc gttcctggat tccgggtgca
    26221 ggctgtcgat accattgctg ccggagatac ctttaacggc gcgttaatca cggcgttgct
    26281 ggaagaaaaa ccattgccag aggcgattcg gtttgcccat gctgccgctg cgattgccgt
    26341 aacacgtaaa ggcgcacaac cttccgtacc gtggcgtgaa gagatcgacg catttttaga
    26401 caggcagagg tgacgcttgg ctacaatgaa agatgttgcc cgcctggcgg gcgtttctac
    26461 ctcaacagtt tctcacgtta tcaataaaga tcgcttcgtc agtgaagcga ttaccgccaa
    26521 agttgaagcg gcgattaaag aactcaatta cgcgccatca gccctggcgc gtagcctcaa
    26581 actcaatcaa acacatacca ttggcatgtt gatcactgcc agtaccaatc ctttctattc
    26641 agaactggtg cgtggcgttg aacgcagctg cttcgaacgc ggttatagtc tcgtcctttg
    26701 caataccgaa ggcgatgaac agcggatgaa tcgcaatctg gaaacgctga tgcaaaaacg
    26761 cgttgatggc ttgctgttac tgtgcaccga aacgcatcaa ccttcgcgtg aaatcatgca
    26821 acgttatccg acagtgccta ctgtgatgat ggactgggct ccgttcgatg gcgacagcga
    26881 tcttattcag gataactcgt tgctgggcgg agacttagca acgcaatatc tgatcgataa
    26941 aggtcatacc cgtatcgcct gtattaccgg tccgctggat aaaactccgg cgcgcctgcg
    27001 gttggaaggt tatcgggcgg cgatgaaacg tgcgggtctc agcattcctg atggctatga
    27061 agtcactggt gattttgaat ttaacggcgg gtttgacgcc atgcgccaac tgttatcaca
    27121 tccgctgcgt cctcaggccg tctttaccgg aaatgacgct atggctgttg gcgtttacca
    27181 ggcgttatat caggcagagt tacaggttcc gcaggatatc gcggtgattg gctatgacga
    27241 tatcgaactg gcaagcttta tgacgccacc attaaccact atccaccaac cgaaagatga
    27301 actgggggag ctggcgattg atgtactcat ccatcggata acccagccga cccttcagca
    27361 acaacgatta caacttactc cgattctgat ggaacgcggt tcggcttaga tttacgctgt
    27421 cttttgatca aattattacc atcggttgtt ttcagaagca tgaacattgc tgctgaagca
    27481 acagtaataa tgcccatcgt gataaacgta tagtggaatt gttcgacagt cgttgtgcct
    27541 tccatccctt cataaacgcg aaggacggcc gcacttacag caacgcctaa actaatcgac
    27601 agttgctgcg tgaccgccag aacactgtta ccgctgctgg cgttgtcatc ggtcagatcg
    27661 gcaagtgtga tggtattcat cgcggtaaat tgcgtcgaca tagccatccc taatataaac
    27721 aacggcaaga tcagcatcca tatcgccatt gccggtgatt gcaaagagaa ctgagcgatc
    27781 attagcccaa taatcaccgt gatccccact aacgtatggc gatagcccag acgacgtaag
    27841 acttgggtaa ccatcgattt tgcaataatg gaacctaacg ctgtcggtgc catcatacag
    27901 ccggcaataa acgcctgata accaaatcct acctgtaaca tcaatggcat aaggaacggt
    27961 acacaaccgg tccccagacg ggttgcaata ttgcctacga taccgatcga gaaagtgcgg
    28021 gttttaaata aatctaatga aattaatggg tttggcgtgt gtcgcgcatg gagaatatag
    28081 agaagcagta acccgatgct ggtgacaatt accgtcaagg caatccagct ggcgacaatc
    28141 ttttccccga atagctctat tccgcttgag aagagaacaa ggctgaggcc aaacagcaaa
    28201 aagccagtga tatcgaatct gcgtcgtgcg gtggtgaaat tgggcatatg tttgcgcgcg
    28261 taaagaaggc ccgcaatacc tatggggata ttgattaaaa atatccagtg ccaggttgcc
    28321 caggtgacca gcacgccgcc aagaacgggg cctaaaattg gccccaccag acccggcatg
    28381 gcgacaaaat tcaatactgg aagaagttca ttacgaggat aagcgcgcag taaggccagc
    28441 cgagcaacag gcatcatcat tgcgccgcct atcccctgaa taacccggaa gacaaccagc
    28501 tgtggtagcg aattagaaag tgcgcaggcc agagaaccca atgtgaacag actcacggca
    28561 agggtaaaaa tgcgacgcgt accgaagcga tcggctagcc atccgcttac cggaataagc
    28621 atcgccaccg tcagcgtata actgatgatg gctgattgca tcgcgagagg agaacgatta
    28681 aggctatgag cgattgcggg taaggcggta ttcagaatag tggcatcaag tgcctgcatg
    28741 aagaaggcca tcgccgcgat ccacggcaaa cccgccatac tgcgattctt tttatcgctc
    28801 attcaatgtc ctgttatcgg gttgtcactt atcaggtgag cgtagcagcg cctgacaagc
    28861 tttaaatgcc gcgtcgccat cgctttggac aatcgcatcg acaatcgcct ggtgcagatc
    28921 cagctttatc actgtgtcgc tggtaattga cgtgaagtaa gtgtgataaa ccgaatggaa
    28981 tagcgaggcg aatgaggtca aaaacggatt ggcgctcatt tcatagatat gctcatgcca
    29041 ggccatatcg acttcgatcc agcgttcacg gcgaaagttc tcttttaatg ccgccatttc
    29101 ggccattaac gtattgagat gcgccttctg ttccgcggtg ccaaccgttg cagccagtag
    29161 gcaagcttgc ggctccagac agatacgcat aaccagaaag tgatcgatga cctgatgaaa
    29221 gttctcttct gtcatccacc aggtaagcaa ttcctgatca agaaaattcc agtttgattg
    29281 tggcatgacc cgggtaccaa ttcgcggtcg cggtaaaacc atcccttttg ccgttaacgt
    29341 tttgaccgct tcgcgtaccg ctgtacgact cactccaaat tgctcgccca gctcaatctc
    29401 accaggcaaa atggtgccgg gttcatattc accttttaag atgcgttgcg ccagcttctc
    29461 agccagaaca tacgaaaggt ttttctgtgc agctaactgt tgtgcgctta aaggcattac
    29521 taatcttcct ttttcttttt attcctcctt agtatgccac caggaagtgt gattacggtt
    29581 gcaaaaacgg caaattgctt gttttatggc acattaacgg ggcttttgct gaaaaaatgc
    29641 gcggtcagaa aattatttta aatttcctct tgtcaggccg gaataactcc ctataatgcg
    29701 ccaccactga cacggaacaa cggcaaacac gccgccgggt cagcggggtt
//
LOCUS       DAFNBJ010000050        29681 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000050 DAFNBJ010000000
VERSION     DAFNBJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.050"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..477
                     /locus_tag="L2X49_002053"
     CDS             <1..477
                     /locus_tag="L2X49_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HBN4797139.1"
                     /translation="GHGIVKFKGEVISAPCSIKPGDEDLTVNLGEVADTVLKSDQKSL
                     AEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQSDLLKNTKETEIGGATGVGVRI
                     LDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSKDATPGNVYAQADYKIAYE"
     gene            537..3035
                     /locus_tag="L2X49_002054"
                     /pseudo
     CDS             537..3035
                     /locus_tag="L2X49_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            3057..3806
                     /locus_tag="L2X49_002055"
     CDS             3057..3806
                     /locus_tag="L2X49_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBN4797140.1"
                     /translation="MRYLNTKNIIAAGVLLSCMNSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKKLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPNEQIQIRMITQEKIAQL
                     PKDRETLFYFNVREIPPKTDKKNVIQVTMQHALKLFWRPKAIELEDDGVMTYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGVTMLPQTQSLMVLPFSHADTQFKNVPSTFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3808..4872
                     /locus_tag="L2X49_002056"
     CDS             3808..4872
                     /locus_tag="L2X49_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4797141.1"
                     /translation="MRYLLTVIAFIMGFSALPVWAMNCYAEHEGGNTVVIGYVPRIAI
                     PSDGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWVNIMMLLASGQDFYNQNSY
                     TLGVTYNGVDYDSTSTRAIAAPECIDVKGAGIYSNTYKNPAVCSGGPEPQLSVTFPVR
                     VQLYIKLAKNANKVNKKLVLPDEYIALEFKGMSGTGTIDTDKNLTFRIRGLNNIHVLD
                     CFVNVDLEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDIGNGLLLKIFHNDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4915..5115)
                     /gene="glgS"
                     /locus_tag="L2X49_002057"
     CDS             complement(4915..5115)
                     /gene="glgS"
                     /locus_tag="L2X49_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBN4797142.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            5384..6013
                     /locus_tag="L2X49_002058"
     CDS             5384..6013
                     /locus_tag="L2X49_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HBN4797143.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            6040..7700
                     /locus_tag="L2X49_002059"
                     /pseudo
     CDS             6040..7700
                     /locus_tag="L2X49_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
     gene            7940..7999
                     /locus_tag="L2X49_002060"
     CDS             7940..7999
                     /locus_tag="L2X49_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBN4797144.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            8315..8374
                     /gene="ibsE"
                     /locus_tag="L2X49_002061"
     CDS             8315..8374
                     /gene="ibsE"
                     /locus_tag="L2X49_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBN4797145.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(8494..9927)
                     /gene="hldE"
                     /locus_tag="L2X49_002062"
     CDS             complement(8494..9927)
                     /gene="hldE"
                     /locus_tag="L2X49_002062"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBN4797146.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(9975..12815)
                     /gene="glnE"
                     /locus_tag="L2X49_002063"
     CDS             complement(9975..12815)
                     /gene="glnE"
                     /locus_tag="L2X49_002063"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBN4797147.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLSEESLSTQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQAEEWQHYSAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(12838..14139)
                     /gene="ygiF"
                     /locus_tag="L2X49_002064"
     CDS             complement(12838..14139)
                     /gene="ygiF"
                     /locus_tag="L2X49_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBN4797148.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDVAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKL
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYAPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            14381..15001
                     /locus_tag="L2X49_002065"
     CDS             14381..15001
                     /locus_tag="L2X49_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBN4797149.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            15065..16303
                     /gene="cca"
                     /locus_tag="L2X49_002066"
     CDS             15065..16303
                     /gene="cca"
                     /locus_tag="L2X49_002066"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBN4797150.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(16484..17305)
                     /gene="bacA"
                     /locus_tag="L2X49_002067"
     CDS             complement(16484..17305)
                     /gene="bacA"
                     /locus_tag="L2X49_002067"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBN4797151.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(17396..17764)
                     /gene="folB"
                     /locus_tag="L2X49_002068"
     CDS             complement(17396..17764)
                     /gene="folB"
                     /locus_tag="L2X49_002068"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBN4797152.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            17869..18486
                     /gene="plsY"
                     /locus_tag="L2X49_002069"
     CDS             17869..18486
                     /gene="plsY"
                     /locus_tag="L2X49_002069"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBN4797153.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(18499..19431)
                     /gene="ttdR"
                     /locus_tag="L2X49_002070"
                     /gene_synonym="dan"
     CDS             complement(18499..19431)
                     /gene="ttdR"
                     /locus_tag="L2X49_002070"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HBN4797154.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            19638..20549
                     /gene="ttdA"
                     /locus_tag="L2X49_002071"
     CDS             19638..20549
                     /gene="ttdA"
                     /locus_tag="L2X49_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HBN4797155.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            20546..21151
                     /gene="ttdB"
                     /locus_tag="L2X49_002072"
     CDS             20546..21151
                     /gene="ttdB"
                     /locus_tag="L2X49_002072"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBN4797156.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            21199..22662
                     /gene="ttdT"
                     /locus_tag="L2X49_002073"
     CDS             21199..22662
                     /gene="ttdT"
                     /locus_tag="L2X49_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBN4797157.1"
                     /translation="MKPSPEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLFPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(22705..23718)
                     /gene="tsaD"
                     /locus_tag="L2X49_002074"
     CDS             complement(22705..23718)
                     /gene="tsaD"
                     /locus_tag="L2X49_002074"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBN4797158.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            23956..24171
                     /gene="rpsU"
                     /locus_tag="L2X49_002075"
     CDS             23956..24171
                     /gene="rpsU"
                     /locus_tag="L2X49_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBN4797159.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            24282..26027
                     /gene="dnaG"
                     /locus_tag="L2X49_002076"
     CDS             24282..26027
                     /gene="dnaG"
                     /locus_tag="L2X49_002076"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBN4797160.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            26222..28063
                     /gene="rpoD"
                     /locus_tag="L2X49_002077"
     CDS             26222..28063
                     /gene="rpoD"
                     /locus_tag="L2X49_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBN4797161.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(28142..28648)
                     /gene="mug"
                     /locus_tag="L2X49_002078"
     CDS             complement(28142..28648)
                     /gene="mug"
                     /locus_tag="L2X49_002078"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBN4797162.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            28773..28848
                     /locus_tag="L2X49_002079"
     tRNA            28773..28848
                     /locus_tag="L2X49_002079"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28806..28808,aa:Ile,seq:cat)
     gene            complement(28901..29665)
                     /gene="nfeF"
                     /locus_tag="L2X49_002080"
     CDS             complement(28901..29665)
                     /gene="nfeF"
                     /locus_tag="L2X49_002080"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HBN4797163.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEDGVASCWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSVLVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFETEQFDPQRVRAAAYWHAK"
BASE COUNT         7510 a         7805 c         7311 g         7055 t
ORIGIN      
        1 ggtcacggta ttgttaaatt taaaggtgaa gttatctctg ctccatgttc tattaaacca
       61 ggtgacgaag atttaactgt aaatctgggt gaagtagcag ataccgtact gaaaagcgat
      121 cagaaatctt tggcagaacc tttcactatt cacctgcagg actgcatgct gagtcagggt
      181 ggaactactt attcaaaagc gaaggttact ttcactacag caaataccat gacgggtcag
      241 tctgatcttc tgaaaaatac caaagaaact gaaatcggcg gcgccactgg cgttggcgta
      301 cgtattctgg acagccagag cggcgaagta actttaggta ctccggtagt aattacgttc
      361 aacaatacca acagctatca ggaactgaat ttcaaagctc gtatggaatc cccaagcaaa
      421 gatgccaccc cgggcaacgt ttacgctcag gctgactaca agattgctta cgagtaatcg
      481 catttggccg ggttcacccg gccacatcat taacggatta atgataagtg gatcagatgt
      541 ataaaaaatt aaaattaacc acaataagcg aattgattaa aaatatttat tgttcattat
      601 ccgttatcat cattggttgt gcgtcagctt atgccgttga attcaacaaa gatttaatcg
      661 aagccgaaga tcgtgaaaac gttaaccttt cccaatttga aactgatggc caattacccg
      721 tcggcaaata ttcactaagc actctgatta ataataagag gacgccaatc caccttgacc
      781 tccaatgggt attaattgat aacctaactg cagtttgcgt gacaccagag caattaacat
      841 tattaggatt tactgatgaa ttcattgaaa aaactcagca aaccctgatc gatggttgtt
      901 accctatcga aaaagaaaaa taaattacaa cttatctcga taaagggaaa atgcaattat
      961 ccatatctgc acctcaggca tggttaaaat acaaagatgc aaactggacg cctcctgaac
     1021 tttgggatca tggtattgct ggggcatttc ttgactacaa tttatatgcc tctcattatg
     1081 caccatatca gggcgataat tcgcaaaata taagttccta tgggcaggct ggggttaatc
     1141 ttggggcctg gcgcctgcgt actgattacc agtacgatca gtcatttaac aatggcaaaa
     1201 gccaggcgaa caacctggat tttccgcgta tttatttgtt tcgcccaatc ccagcaatta
     1261 atgcaaaact aactataggt caatacgata ctgaatcctc tattttcgac tctttccatt
     1321 tttctggcgt ttcgttgaaa agcgatgaga atatgttgcc gcctgatcta cgtggttacg
     1381 caccgcaaat cacgggtgtt gcacaaacga atgcaaaggt cactgtctca cagaacaacc
     1441 gtattattta tcaagaaaat gttcctcctg gcccatttgc tattactaat ttattcaata
     1501 cattacaggg gcaactggac gtcaaggttg aagaagagga cgggcaagtc acgcaatggc
     1561 aagttgcatc taatagcatt ccttatctga cgcgtaaagg gcagattcgc tacactactg
     1621 caatggggaa accgaccagc gttggcggcg attccttaca acagcccttc ttctggaccg
     1681 gtgaattctc atggggttgg ctgaacaatg tatccctgta tggtggttca gtattaacaa
     1741 accgtgatta tcaatccctg gctaccggag ttggttttaa ccttaactcg ttgggctcat
     1801 tatcctttga tgtcacgcga tctgatgctc agttacataa tcagaataaa gaaacgggtt
     1861 atagctaccg tgctaactat tcaaaacgtt ttgaatctac cggtagccag ctcactttcg
     1921 ctggctaccg tttctctgat aaaaactttg tgtcgatgaa tgaatatatc aatgacacta
     1981 accattacac gaattatcag aatgaaaaag agagttatat tgtcacgttt aaccagtatc
     2041 ttgaatcatt gagattaaat acatacgtaa gtctggctcg taatacttac tgggacgcca
     2101 gcagtaatgt gaattattca ttatcactta gccgcgattt tgatatcggc ccattaaaaa
     2161 acgtatccac ctcactaaca tttagccgaa taaactggga agatgacaac caggatcaac
     2221 tgtacctaaa tatttcaatt ccctggggaa ccagtagaac attgagctat ggtatgcaac
     2281 gaaatcagga taacaacatt tcgcatactg cttcgtggta tgactcttcc gatcgaaata
     2341 attcctggag cgtttctgct tcaggcgaca atgacgaatt taaagatatg gaggcgtcac
     2401 tgcgcgccag ttatcagcat aataccgaga acggtcgtct ctatctctcc ggtacatcac
     2461 agcgggacag ctactattct ctgaatgcca gttggaacgg ttcattcact gcaactcgcc
     2521 acggcgccgc tttccatgac tatagtggta gtgctgactc gcgttttatg atcgacgcag
     2581 acggcgctga agatattccg ttgaataata aacgcgcggt aactaatcgt tatggcatcg
     2641 gagttattcc atcagtcagc agttacataa caacatcatt aagcgttgat acccgaaatc
     2701 tgccagaaaa tgtggatatc gaaaactcgg ttatcaccac caccttaacc gagggtgcta
     2761 ttggctacgc caaacttgat acccgcaagg gctaccaaat cataggggtt attcgcctgc
     2821 cagatggtag tcacccacca ctggggatta gcgtaaaaga tgaaaccagc cacaaagaat
     2881 taggactggt tgctgatggc ggttttgtat acctcaacgg cattcaggat gacagcaaac
     2941 ttactttacg ctggggtgac aaatcttgct ttattcaacc acccaagagc agcaacttaa
     3001 ccaccggaac ggttatttta ccgtgtatta gctaaaatta attcaggatg aaactaatgc
     3061 gctacttaaa tacaaagaat atcattgcag caggagtatt actctcctgc atgaacagca
     3121 ttgcctgggg tgccatcatc ccggatcgta cccgtataat aatgaatgag tcagataaag
     3181 gcgaagcatt aaaacttact aatcaaagta aaaaactgcc ctatctggcc caaacatgga
     3241 tagaagatac gaaaggcaat aaatctcgtg actttattgt tacggttcct cctatggtgc
     3301 gtttaaatcc caatgagcaa attcagatta gaatgataac tcaggaaaaa attgctcaac
     3361 ttcctaagga cagagaaact ctgttctatt ttaacgtgcg agaaataccg ccaaaaacag
     3421 ataaaaaaaa tgtgattcaa gtgactatgc agcacgcttt gaaattattc tggcggccaa
     3481 aagccattga gttagaagac gatggggtaa tgacttacga aaaagttgaa atcatccgta
     3541 gaaatgatgg ttcgatccgc tttaacaata aaatgcctta tcacgttacg ctgggttata
     3601 tcggaacaaa tggggtcacg atgttaccgc aaacgcaaag cttaatggtc ctccctttta
     3661 gccatgcaga tacgcagttt aaaaatgttc catcaacttt tcaggttggt tatatcaatg
     3721 attttggtgg cttaagtttt tatgaaataa actgcccaac agtgaataat tcttgtaatg
     3781 tttccgtagc caaacgagat aaataaaatg cgctacttat taactgtaat tgcatttatt
     3841 atgggtttta gtgcgttacc tgtatgggcg atgaactgct atgctgaaca tgaagggggg
     3901 aataccgtag tcataggcta cgtaccaaga attgctatcc ccagcgatgg taaaaaaggt
     3961 gataaaatct ggcaaagcag tgaatatttt atgaatgtct tctgtaataa tgcactaccc
     4021 gccccatcac caggagaaga atacccatct gcatgggtaa atataatgat gttgttagca
     4081 tcaggtcagg acttttataa tcaaaactca tatactcttg gtgtaaccta taatggagtc
     4141 gattatgatt caacctccac acgagctatc gcagcaccag agtgcattga tgttaaaggc
     4201 gcagggatat acagtaatac ctataaaaac cccgctgtct gtagcggtgg tcctgaacct
     4261 caattgtcag taacttttcc agtacgtgta cagctgtata ttaagctggc taaaaatgct
     4321 aataaagtaa ataaaaaact tgtattacct gacgaatata tcgccctgga gtttaaaggt
     4381 atgagtggca caggaactat agacacagac aaaaatttga ccttcagaat tcgtgggtta
     4441 aataatattc atgtccttga ctgctttgtt aatgttgatc tggaaccagc tgatggcgtt
     4501 gtcgactttg gtaaaataaa ttcccgaaca attaaaaata ccagcgtgag tgagacgttt
     4561 agcgtagtca tgaccaaaga tccgggtgcg gcctgtactg agcagtttaa tattttaggg
     4621 agttttttca ctacggatat tttgagtgat tatagccatc tggatatagg taatggtctg
     4681 ctattgaaga tatttcataa cgatggaaca gcaacggaat ttaaccgctt ctcacaattt
     4741 gcttcttttt catcgtctag tgcgccttcg gtcaccgcac cattcagggc agaactgagt
     4801 gcgaacccgg cagaaacggt tgttgaggga ccgtttagta aagacgtaat cctgaaaatc
     4861 acctataact agtatctaat acaaacacta aaacgggcca tcaggcccgt tatatcagtg
     4921 ctctaactcc agctctcttg cctgcatcat ctgttgacaa taccaggcat aacgtgcgca
     4981 aaaccaggtt ctatgttctt catccatgtt accagtaacg gccagaatat cgaccgggcg
     5041 accttctgcg tgcattctgg caaaactacg cgccaggaaa tcgaaattat ttaaagaatt
     5101 aagactatga tccataagca catcctccgg ttatcgcata ccttttaagg ccatatgctt
     5161 tttctgattt cagtattccc caatcagtgt agtgccttta aacataacgt gcgtaaatat
     5221 attgtccccg atcacacttt ttagtgaaaa ataaaaaacc atattaaaag actttctctt
     5281 ccagaacttc tctaacctgt gttattatta acgcagataa atgtaagcaa ggaaactatt
     5341 acaaagtaaa tattgcgtca cgaaatggac attggagtga tatatgattt tattcgccga
     5401 ctataacacc ccttatcttt tcgctatttc ctttgtactg ctgatcggcc tgttagaaat
     5461 ttttgcgctt atctgcggtc acatgctctc cggtgctctt gatgcacatc ttgaccatta
     5521 tgattccata accacaggtc atattagcca ggcacttcat tatctcaata ttggaagatt
     5581 gccagctctc gtcgttctct gcttactggc tggttttttt ggtcttattg gcatcctgtt
     5641 gcagcatgcc tgcatcatgg tctggcaatc accactgtcg aacctgttcg tggttcctgt
     5701 cagtttactg tttacgatta ttgcggtgca ttataccggt aaaatagttg cgccctggat
     5761 tccacgcgac catagttcag ctattacaga agaagaatat attggtagca tggcgttaat
     5821 taccggtcat caagccactt caggtaatcc ctgcgaagga aaactcaccg atcaatttgg
     5881 ccaaatacat tatttactac tggagcctga agaaggaaaa attttcacta aaggagacaa
     5941 agtgttaatt atttgccgac tttctgcaac gcgatatttg gcggaaaaca atccctggcc
     6001 ccaaatattg taatttatta atcaaaagga aatttaataa tggatgatat tgttaattct
     6061 gtgccctcct ggatgtttac cgcgattatt gccgtatgca ttctgtttat tattggaatt
     6121 attttcgcca ggctctatcg tcgcgcttcg gcagagcaag cttttgttcg tactggttta
     6181 ggtgggcaaa aagtggtaat gagcggtggc gcaatcgtga tgccgatctt tcatgaaata
     6241 atccccatca atatgaatac tctgaagctg gaagtcagcc gctcaaccat tgatagcctg
     6301 attacgaaag atcgtatgcg cgtcgatgta gtagtcgctt tctttgtgcg ggtaaaacct
     6361 tcagtagaag ggattgccac cgctgcccag acgctggggc aacgcaccct gtcgcctgaa
     6421 gacttacgta tgttggttga agataaattt gtcgatgccc tccgtgcaac agctgcgcaa
     6481 atgaccatgc atgagttaca ggatacccgc gagaacttcg tgcagggggt gcaaaataca
     6541 gtggcagaag acctgtcgaa aaacggtctg gaactggaga gcgtttcact taccaacttt
     6601 aaccagacct cgaaagaaca tttcaatccg aacaatgcct ttgacgccga aggtttaacc
     6661 aaactgactc aggaaacaga gcgccgtcgc cgcgaacgta acgaagttga acaggatgta
     6721 gaagttgcgg tgcgtgaaaa aaatcgcgat gcgctatcgc caagctggag attgaacagc
     6781 aagaagcgtt tatgacgctt gagcaggagc agcaggttaa aacccgtact gccgaacaga
     6841 atgcacgtat tgcggctttt gaagctgaac gtcgtcgtga agcagagcag acacgaattc
     6901 tggctgaacg acagattcag gaaacagaaa tcgaccgcga acaggccgtc cgctcaagaa
     6961 aggttgaagc tgaacgtgaa gttcgcatta aagagatcga acagcagcag gtcaccgaaa
     7021 tcgctaacca gacgaaatcg atcgctattg ccgccaaatc ggaacaacag tcccaggcag
     7081 aagcgcgtgc taatctcgca cttgcagaag cggtaagcgc ccaacaaaac gtagaaacca
     7141 ctcgccagac tgccgaagcc gatcgtgcta aacaagttgc cctaatcgct gccgcgcagg
     7201 atgcagaaac caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa gaagccgcag
     7261 agatgcaggc ggcggctatc gttgagttag ccgaagctac acgtaaaaag ggtctggcgg
     7321 aagcagaagc acaacgtgcg ctgaacgatg ctatcaacgt actttctgat gaacaaacca
     7381 gccttaaatt caaactggcc ttgttgcagg cgctgcctgc ggtaatagag aaatccgttg
     7441 agccgatgaa gtcaatcgac ggtatcaaga ttattcaggt cgatggtctg aatcgtggcg
     7501 gcgctgcggg tgatgcgagt acaggtagcg ttagtggagg aaaccttgca gagcaggcat
     7561 tgtctgccgc cctttcttac cgcacacagg caccgctgat tgactccttg ctcaatgaaa
     7621 ttggcgtttc aggcggctca ctggcggcat tgacttcacc cttaacctca acaactcccg
     7681 tcgaagaaaa agcagagtaa accctcttcc aggaataatc cctggcccaa aaagccgggg
     7741 attttttata tctgcgttcc gctaaaaagt gcaaatgatc aggttgttgc agcattttgc
     7801 gtgacggctc ggggaaggca aaactgcatc agagaaagca ttgcgtgggg tctggcgctc
     7861 atcaacaatc ggggagtcag cagggggctg aaacgggaaa gcccctcccg aagaaggggc
     7921 cttacagaag gaaagggtta tgatgaagct cgtcatcata ctgattgtgt tgttactcgt
     7981 aagtttcgca gcttattaac agccaatcag aggggggaga aatcctccct cacccttgtt
     8041 cctttacttt aggttgaaaa aaaatcagcg tcaagaaggt ttcattaaca catatccctg
     8101 gcccaaaaag ccggggattt tttatatctg cgttccgcta aaaggtgcaa atgctcagga
     8161 cgttgcagcg ttttgcgtga ccgctcgggg aaggcaaaat tgcctctggg aaagcattgc
     8221 gcggggtccg gcgctcatca acaatcgggg ggcagcaagg ggctgaaacg ggaaagcccc
     8281 tcccgaagaa ggggccttgt ataaggaaag ggttatgatg aagttcgtca tcatactggt
     8341 tgtgttgtta ctgttaagtt tcccgactta ctaacaactc atcagagggg ggagaaatcc
     8401 tcccttaccc ttgttccttt actctaggtt gaaaaaacaa cagcgtcaat aggcctgcca
     8461 tgtacgaagc gagatctgtg aaccgctttc cggttagcct tttttatcct gttggatctt
     8521 cttgatgatg ttggtcgtcg agcaaccgtc ttcaaagttg agcaccaaca cttcgccacc
     8581 gttggcccag acttctttac tcccggcaat ctcttctggt ttatagtcgc cgcctttcac
     8641 cagcagatct ggcaagatcc cggcgatcaa gcgctgcggc gtgtcctctt caaacgacac
     8701 cacccagtcg accgcttcca gtgcgcccag cacaatcata cgctgttcga gcgggttaac
     8761 ggggcgggaa tcccctttca gccgtttggt ggaggcatcg ctgttcacag cgacaatcaa
     8821 acggtcaccc agtttacggg catttgccag ataagagacg tgcccggcgt gcaggatgtc
     8881 aaagacaccg ttggtcatca ccactttttc accacgttta cgcgctgccg ctacggccag
     8941 cttcagttcc tcttcggtca tcacgccaaa gcctgtatct gcacgtccac gtacagcatt
     9001 ttccagctcg atcggcgaaa ccgtggaggt tcccagtttg ccgaccacca cgccagccgc
     9061 cgcattggca aagaagcagg cttcttccag cgaattaccc gctgccagcg ttgccgccag
     9121 gacgccaatc accgtgtcgc ccgcaccggt aacgtcatac acttcctgcg cctgggttgg
     9181 catatgcagc ggcgctttac ccggttgcag cagcgacata ccctgttcgg aacgggtcac
     9241 taacagagcc gagagttcgt aatcggcaat cagtttcatg ccgcgctcaa caatctcttc
     9301 ttcggtctta catttaccga caacagcttc aaattccgag agattcggcg ttaacagcgt
     9361 agcgccgcgg tagcgctcaa aatcggtacc ttttggatca atcagcaccg gaacacccgc
     9421 tttacgcgcc agttggatca tctgctgtac gcttgccagc gcacctttgg cgtagtcaga
     9481 aagcaccagc gcgccaatcg aactcagcgc ctgattaatc cgctcgtgca gcggctgcgg
     9541 atcaacacct tcgaaacctt cttcaaaatc cagacggatc agctgttggt tgcgggaaag
     9601 tacccgtaat ttggtaatgg tcggatgcgt cggtacagaa acgaagtcgc atttgacgtt
     9661 gacgtcggcc agagatttac tcagcgcgcg cgctgcatcg tcaatgcccg tcaacccgac
     9721 caggcgtgca ttagcaccga gagaagcgat attcatcgcc acgttagccg cgccgcccgg
     9781 acgttcttcg atggtattca ctttaaccac gggcaccggc gcttccggcg agatacgact
     9841 ggtggggccg taccagtaac gatccagcat cacatcacca accaccatca ctcctgcacg
     9901 ttcaaactct ggcagcgtta ctttcattcc tgtctcctga gagattcaaa atttgcgcgc
     9961 gataatacca tacttcattc ttccaccagc cacttctgcc agcttgcccg caccagttca
    10021 cgctctgcgg tgaagcaatc ctccgacaca tggcccggca attcctgtaa tgccagatga
    10081 tgaagttcat cgcgaagcgt agtgtaagca cgggtcagcg ccatcgcttc ctgctcttcc
    10141 ataatgtcgt tttgcgccag tagttccaga atacgcacgt tgtctgacca gcgcgttaac
    10201 ttcggttttt catgagcgta gcgcaacacc agatattggg taataaattc gatatcggta
    10261 attccccctt catcagcttt gatatcaaag cgatcgcgat gtttattgcc gagatgagcg
    10321 cgcattttct cgcgcatttc ccgcacttcc gtttgcagag ttttaccttc acgcggcagc
    10381 gtcataatct cgcgacgcac tgcgtcaaag tgcgcggtga gctgcggatc gccgtacact
    10441 acacgcgcac gcaccagcgc ctgatgttcc cacgtccagg cctcgttttt ctgataatcg
    10501 gcaaatgctt ctgcggatgt caccagcatt cccgccgccc cggacggacg cagtcgagca
    10561 tccacttcat acaaaatgcc ggaagaggta cgcgtactga acagatgcat aatgcgttgc
    10621 gccagacgca gataaaactg ccgcccgtcg atttcccgct caccgtcagt catcgcatcc
    10681 attgggcaat catggaggaa gataaggtca agatcggaac tgtagcctaa ctcccagccg
    10741 cccagcttgc cgtagccgac caccgcaaaa ccacgccctt cgcgttcgtt caggtgattc
    10801 ggcttaccgt agcgggcaac catttgaacc cacgcctgct gaacgacggc atctatcatg
    10861 gcttccgcca gccaggttaa gtgatcgctc actttcatca ccggtagcgt accggcgata
    10921 tccgctgcgg cgatgcgtaa cagctgcgcc tgtttgaact gacgcagcgc ctcaagctgt
    10981 tgctcttcgt catcttccgg cacgcgcagc aaatactggc gcaactcatc gcggtaggca
    11041 tcggtcgccg tcggctggta aagggtgttt ggatcgagca attcatccag caataatgga
    11101 taacgcgcca gctggctggc aatcatcggc gacgcggcac acagagaaat caaatgttta
    11161 agcgccgcgg ggaattcact gagcaattct aaataggtgg tgcgggtaac aatccccacc
    11221 agcaaggcgg taatgcgcga cagcgtaacg gcagcgtctt cacgcgcaca gacatcactt
    11281 agcagatgcg gcatcagatg gtcgagcacc tgacgtcctc gcggcccgat ggtgcgctta
    11341 tccagctctt tgcggaaatc ggcaatcagc gttagcacct gtttgcgatc atcctcgcta
    11401 agatgcgcca gcactggcgt agtgtcatct tcctgcaacg catcctgcca cagctcacgc
    11461 cactgttccg acagcgactc ttcctgagtt tcactttcgt cgtcgccaat caattcatta
    11521 aacacccggc gcacattggt catatgtgcg gtcagcgccc cggtcaattg cggccagtca
    11581 gcaaagtcca tcgcccacgc cagccgcgca cgattaagct catcagaagg aagcgtctgg
    11641 gtttgttcgt cgttaatgct ttgcagcagg ttttccagac gccgcaggaa cagatacgcc
    11701 actcgcaatt gttcagcatc gttttcagaa agcagatgca gctcggcaat ggcgctgagc
    11761 gttggcagta aagagcgcga ttgcagcgac ggttcgcgtc cgccgcgaat gagctggaac
    11821 acctgaacga taaattcaat ttcgcgaatg ccgcctgcgc cgagtttgat attgtcggtc
    11881 aaaccacgtc gacgcacttc acgggcaatc atccctttca tgttgcgcag cgactgaatc
    11941 acgctgaaat cgatgtaacg acggaaaaca aacgggcgca gcatcgcacg caactcgtta
    12001 gcatagacgc cttcgctatc gcccataatc cgcgccttga ccatcgcgta acgctcccag
    12061 tcgcgcccct gctcctggta ataatcttcc aacgcggcaa agctcagcac cagcgggcca
    12121 ctttcgccaa acggacgcag ccgcatatcc acgcgataga cgaagccatc ctgcgttggt
    12181 tgatccagca ctttaatcag ccgctgcccc atgcgggtaa aaaactgcgc gttatccagt
    12241 tcccggcgtc caccctgcgt acaaccatgt tccggccagg caaaaatcag atcgatatca
    12301 gaggagaaat tcagttcccc gccgcccagc ttacccatgc ctaaaatcag cagcggttgc
    12361 gcttcgccct gcgcattgca cggcgttccc cactcgcggc agcaggcgtc atacagccag
    12421 tcgcgcgccg caacaatcag cgtctccgcc agatgactga gttgctgcaa tatgctctct
    12481 tcagtaacca aagccagcgt ttgcgcccag gcgatgcgca ccataatgcg ccgccggaat
    12541 agccgcagct cgcgcattaa cccggcttcg tcactcacat tactgagcgc ctcctgcaac
    12601 catgcagagt aatgctgcca ttcttcggcc tgcggcggtt ggctttccag ttccgtcagc
    12661 cactctggat gcgcaatcac gctgtcctgc acaaaatcac tgaaagtaag taccgacttc
    12721 gcctgtgtac taagtgattc ctcggataat ggctctggca gccgctcaac aacggtctgc
    12781 cagtactgct gtaacggtga agagagcggc ttcataagcg attttatcct tgcctgatta
    12841 acgttttccg ctgtgcaacc agaacggttc ctgattgttt gcctcattac ggaaatgttc
    12901 aatttcaatg cgttgcccgg tcgcaatagc gtgacgcagc ccctgccagt tctccagcca
    12961 ggcttgcgcg acgacaggag catagtaacc cgccagcaac agtattgagt caatatcacg
    13021 cgtcagacgt ggcaactggt cacggtagcg atcgcccaac ggctggcaga aaacgctttt
    13081 cagttcagcg gcatggcggg aaagatggat atcggcaaag cgtttgaagg aatcgctcaa
    13141 tttactctgc gctttggcat ctaaaaacgg ctgccaggct ttgcttacca gccattcggt
    13201 caacgccagc ttcgccattg ccgttttggt agagtagacc gccgtcacgg cagaaaccgc
    13261 agaagcgatg gtcgcttcgc attgagtcag caaatcacgt aagtgagtgc tcgctttacg
    13321 cggcacaatg ccaccgaaca gcatcagtgt atgacggacc aggccaatgg ctgccagcac
    13381 ctgctctttc gccacatcgt tgccgcgtac ccacagttct tcatgatact gccattgcgc
    13441 taacgccagc tcgagcgccg cttccagccc ctgttccaca tcggcttttg ccgcaacatg
    13501 caaaatggtg gttggtttga tttcacgcgc cggattgccc tgcgccagat gatagccacg
    13561 cgccgctttg ctcaggctgc cctggcgtaa tccggtttgc gataccagtt ggttcgccag
    13621 tttcagcacc gcgcgtgtgt cgccgctaag cagttccagt tccagctcac agataggttc
    13681 agcaaattca cccgctttca cttccccctg gtcgagggcg atttcaattt ggctaccatc
    13741 gaccgccacc agccattttt cgcgataaaa atcggtgctg aacagcggct gcacgcggga
    13801 ggcgagatcg gcgggcaatt cgccgttcgg ccagacttcc gtcggtaact gcgccaggtc
    13861 gagcgtcggt tcgctcaacg ccacgttata ttccgggcgc tgatgtaagc cgcctgtcac
    13921 tcttcctgca actttcatgg tcatctcata gcgaccgttt tcgccacgaa tacgtaagcc
    13981 catatcgtgc ccacgcagcc agttatccgg cgtttcgtag taaatattca gcaactgcac
    14041 ggggtcatgg tgctcgccgc ccagcgtatt gagatggtca cgcaacgcct caacggcact
    14101 gtgattaaca ataaacttta attcgatttc ctgagccatg gcctttactt atcggttatg
    14161 tcacataagt gacgatgaac ggcgaactta atgcgatctt ttttgtcagt agatagtatt
    14221 ttgcgccaaa ttgccatgca acgggcaatt tgacgggcgt aaaagtttga agcagtggca
    14281 agacctgaca cagaggattc cgattgatgt cgaatgcttt gcgtagcgac actgatgcca
    14341 ctactatcgt tccactttcc atgacaataa cgacagcctg atgccaaaat tacgcctgat
    14401 cggattaact ttactcgcac ttagcgcgac tgccgtctca cacgctgaag aaacgcgcta
    14461 tgtttctgac gaactgaata cctgggtccg tagcggtccg ggagatcatt atcgcctcgt
    14521 gggcacggtt aacgccggcg aggaagtgac cttattacaa actgacgcca acaccaatta
    14581 tgcccaggtg aaagacagct ctggccgtac cgcctggatc ccgttgaaac aacttagcac
    14641 tgagccaagc ctgcgctccc gtgtgccaga tctggaaaat caggtcaaaa ccctgaccga
    14701 taaactcacc aatatcgata acacctggaa tcagcgcacc gcagaaatgc agcaaaaagt
    14761 ggcgcagagc gacagcgtga tcaacgggtt aaaagaagaa aatcagaaac tgaaaaacga
    14821 gctgattgtc gcgcagaaaa aggtcgatgc cgccagcgta cagctggatg acaaacagcg
    14881 caccatcatc atgcaatggt ttatgtatgg cggtggcgtg ctggggcttg gcttgctgct
    14941 cggtctggta ctgccgcacc tgatcccaag ccgcaaacgc aaagatcgct ggatgaacta
    15001 aatcgccttc ttcgttgcac tgtcttatta ttaggaattg tgtatttgac gaagggatga
    15061 tggcgtgaag atttatctgg tcggtggtgc tgttcgggat gcattgttag ggctaccggt
    15121 caaagacaga gattgggtgg tggtcggcag tacgccacag gagatgctcg acgcgggcta
    15181 ccagcaggta ggccgcgatt ttcctgtgtt tctgcatccg caaacgcatg aagagtatgc
    15241 gctggcacgt accgaacgga aatccggttc cggttacacc ggttttactt gctatgccgc
    15301 accggatgtc acgctggaag atgatcttaa gcgtcgcgat ctgaccatta atgcgctggc
    15361 ccaggacgat aacggcgaga ttatcgaccc gtacaacggt ctgggcgatc tgcaaaatcg
    15421 tctgttgcgc catgtttccc ccgcttttgg cgaagatccg ttacgcgtat tgcgcgtggc
    15481 gcgttttgct gcgcgttatg cccacctcgg ttttcgtatt gccgatgaaa ctctggcgtt
    15541 gatgcgcgag atgacccatg cgggtgaact ggaacacctg acgcctgaac gggtatggaa
    15601 agagacggaa agcgccctca ccacccgcaa tccacaggtg ttcttccagg tactgcgcga
    15661 ttgcggcgca ctgcgcgttt tattcccgga aattgacgca ctgtttggcg ttccggcccc
    15721 tgccaagtgg catccggaaa tcgatacggg tattcatacc ttaatgacgc tctcaatggc
    15781 ggcgatgctg agtccgcagg tcgatgtccg tttcgcaact ttatgccacg atctcggtaa
    15841 agggctgacg ccgccagaac tctggccgcg tcatcatggt catggcccgg cgggtgttaa
    15901 gttagtggaa caactatgcc agcgtctgcg cgtgccaaat gaaattcgcg atttagccag
    15961 actggtggct gagtttcacg atctcatcca caccttccca atgctgaacc caaaaaccat
    16021 cgtcaaatta tttgattcca tcgatgcctg gcgtaaaccg cagcgtgtcg agcaactggc
    16081 gctgaccagc gaggctgacg tgcgcggcag aaccggtttt gaatcagcgg actacccgca
    16141 aggccgctgg ttgcgcgaag cctgggaagt ggcgcagtca gtgccgacaa aagccgtcgt
    16201 tgaagcggga tttaaaggtg tggagattcg cgaggagctg acccgacggc ggattgcggc
    16261 ggtagccagc tggaaggaac aacgttgccc aaagcctgaa tgagtattgg gcattttgcc
    16321 ggatgcggcg taaacgcctt atccggctac atgtcaacga cagttgtagg cctgataaga
    16381 cgcgcaagcg tcgcatcagg cgttgattgc cggatgcggc gtgaacgcct tattcggcct
    16441 acgcaatcgt tgacaacgcc aagcatccga cactattcct caattaaaag aacacgacat
    16501 acaccgcagc cgccacaata aagcgataaa tggcgaacgg gataaacgaa atgcgcttaa
    16561 tcaattgcag gaaggtttta atcgctatca gcgccaccac aaaagcggtg ataaacccaa
    16621 cggcaaacat cgggatatcg ccggttgtca ggaagcccca gcttttgtag agatcgagcg
    16681 ccgttgcgcc catcatcatc ggtaccgcca gcaggaacga gaactcggaa gcagcgtaac
    16741 ggctcacgcc catcagcatc ccacctgaaa tggtggcccc ggaacgggag aaacccggcc
    16801 acagcgccag acactggaaa cagccaatca taaacgcctg acgataggtc atatcatcaa
    16861 gacccggcgc acgcggctct ttcggcttca ggcattcggc ggcaatcagc aacaaaccgc
    16921 caacgaccag tgcatacatc acatttattg ggttaaacaa tgacttaatc gtgtcgtgga
    16981 acaacagccc cagtaccacc gccggaatca tccccagcaa aatgtggatc agcgttaaac
    17041 gacctttgct ttctccttcg tgctgcaacg ggcggccaaa gtggatgcca atcaggccaa
    17101 acagacgccg ccagaacatc actactaccg ccagaattga tcctaactgg atcacaactt
    17161 caaaggtttt cgccgtgtcg ccctcgaacc ccaacaagtg accgacaata atcatatggc
    17221 ccgtgctgga taccggcaga aattctgtca atccttcgac cacacccaat attgccgcta
    17281 tcagcagcga gtgcatatcg ctcatcaata aacccctaaa ttattaaaat gtaccgcttg
    17341 tccggactac tgcagtaaga ccaggttata accgtttggt ttaacagctg taaaattaat
    17401 tattttcttt cagattattg ccacgctcaa tgattacacc aacattcgcc gcccgcgcca
    17461 ctgcgcctgg cttgctgagt ttgatgcgca cccacggcga gttgaagcgt gctaacagca
    17521 gctccgccac ctcttcagcc acgcgttcca ccagcgcaaa acgcgccccc tcgacgtggc
    17581 tgaccaccgt ttctgcaatg tcagcgtaac tgaggcaatc cgccacatca tcacttttcg
    17641 ccgctttacg gttatcccat gccatttcga tatcgaacac taacttctgt tcgatggtct
    17701 gttcccagtc gtaaacacca atagtggtga ttaccgaaag ttgctctata aatacaatat
    17761 ccatcacgtc ctgcctgctt tttggctaac cggataccac ttccggcgaa atgtgcgtat
    17821 tatccacaga ttcatcgttg aacacgaatt ttcaaaacgg aacagcttat gagtgcaatc
    17881 gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca tttccagtgc cattctggtt
    17941 tgccgcttgt gtgggctgcc cgatccgcga accagcggct ccggcaatcc aggcgcaacc
    18001 aatgtgttac gtatcggtgg caagggagca gccgtagcag tactgatttt cgacgttctg
    18061 aaaggaatgt tgcccgtctg gggcgcgtat gaattaggtg tcagcccctt ctggctaggc
    18121 ttaattgcca tcgccgcctg tcttggacac atctggcccg ttttcttcgg atttaaagga
    18181 ggaaaaggcg ttgctaccgc ttttggtgcc atcgcaccca ttggctggga tctcaccgga
    18241 gtaatggcgg gaacctggtt actgaccgtg ctattgagcg gatactcgtc gctgggagcg
    18301 attgtcagtg cactgattgc tccgttttat gtctggtggt ttaagccaca attcaccttc
    18361 ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc atgacaacat ccagcgtctg
    18421 tggcgtcgtc aggagacaaa aatttggacg aaattcaaaa gaaagcgcga aaaggatccc
    18481 gagtgatttc tggtggatct acatgacctg atagccttca tcgggcttgc ccagccgttg
    18541 ctggcaccat gccgccagaa attccacgca gacacgtaat ttcatgctgc gatagagcgg
    18601 ctcccggtaa acagcccaga tattggcgct ttgtgcatac tccggcaata cttgcaccag
    18661 tttgccactc tccagaaacg gcaacacatc ccactcggaa cgcagcataa tccctttgcc
    18721 ctccagcgcc cattgcagca ctatctcgcc gctattggag gaaagatgcc cgctaacctt
    18781 caccgatttt ttctcctgac cgttccccaa ctcccatatt ccgtgggtca tatcgcgctc
    18841 tttggtcacc agacagtcat gacgacttaa ttcctgtaat gattgtggct gcggatattt
    18901 ttgcagatac tcaggtgctg cgcataatat tcttttattt ttcgttaaca gatgcgcaat
    18961 ataataatca ggaatttcgt cattaatacg aatatccaga tcaatattat cctgcactaa
    19021 atcaatttgc cgatcgaaca attcaaaatg cacctgtaac tcaggataat tgcgcatcag
    19081 ttcggtaatg gctggcgcaa tatggctgcg cccaaaacca aaactacagc caatacgaat
    19141 catcccctcc gggcgcgttt tgatttgcgt gacgtcatcg accagtcgct gatattgggt
    19201 gaggatttcc agcgcatgtt cgtaacaacg ttggccactt tcagtcagcg ccacgccgcg
    19261 agcggagcgg ttcagcagcg ttgttgccag cgtattttca aggatttgga tccgcttggt
    19321 gacaaatgca ggcgtttgcc caagcgtcgc cgctgcggcg ctaaaactcc cgctgtggac
    19381 gatttctacc agtacctgca aatctttggc taaaggccag ctattcagca tcgttaatta
    19441 tccgcagttg tgataagcgc agtgtatttc gcaaaacatt gccattccct gctctggcgc
    19501 aatattcatt ctttttttcg cgttgattca cgaactgtta attacatatt cacggtggca
    19561 aaaaatataa aaccacattt ttagtggtag tttgtggcgg tgaatttttc cagacaaata
    19621 cataaactgg agttgccatg atgagcgaaa gtaataagca acaggcagtg aataagttga
    19681 cggagattgt cgctaacttt accgccatga tttctacccg aatgcctgat gacgtggtgg
    19741 ataaactaaa acagctaaag gatgccgaaa cgtcgtcgat ggggaaaatt atctaccata
    19801 cgatgttcga caacatgcaa aaagcgattg acctgaatcg tcctgcctgt caggacaccg
    19861 gggagattat gttcttcgtt aaagtcggtt cccgcttccc actgcttggc gagctgcaaa
    19921 gcatactcaa acaagccgtg gaagaggcaa ccgtcaaagc gccactacgt cacaatgcgg
    19981 tagaaatttt tgacgaagtg aacacaggca aaaataccgg cagcggcgta ccgtgggtca
    20041 cctgggatat cgtccccgac ggtgacgatg cggaaatcga agtttacatg gcaggcggcg
    20101 gctgcacgct accaggccgc tcgaaagtgt taatgccgtc agaaggctac gaaggcgtgg
    20161 tgaaattcgt cttcgaaaat atctccaccc tcgccgtaaa cgcctgtcca ccggtactgg
    20221 tgggcgtggg catcgccacc tcggtggaaa ccgccgccgt actctcgcgt aaagccattt
    20281 tgcgtccgat tggcagccgc catcccaatc caaaagcggc agagctggag ctacgcctgg
    20341 aagaaggact caaccgtctg gggattggtc cacaagggct gaccggcaac agttcagtga
    20401 tgggcgtgca tatcgaatct gccgcccgcc atccgtcaac catcggcgtt gctgtctcta
    20461 ccggctgctg ggcgcatcgt cgcggcacgc tgctggttca tgccgatctc acctttgaaa
    20521 atctgtctca cacccggagc gcgttatgaa aaagatcctg acaaccccga tcaaagctga
    20581 agatctgcaa gatattcgcg tcggcgatgt gatctacctg accggtacgc tggtgacctg
    20641 ccgcgacgtt tgtcaccgcc gcttgatcga actgaaacgt ccgatccctt acgatctcaa
    20701 cggcaaagcg attttccacg ctggccccat cgtgcgcaaa aacggcgaca aatgggagat
    20761 ggtctccgtc ggtccgacaa ccagtatgcg tatggaaagt tttgaacgtg aatttatcga
    20821 gcagaccggc gtgaaactgg tggtcggcaa aggcggaatg gggccgctga ccgaagaagg
    20881 ctgtcagaaa ttcaaggcgc tacatgtaat tttcccggca ggctgcgcgg tgctggcggc
    20941 aacccaggtg gaagagattg aagaagtgca ctggacagaa ctcgggatgc cggagtcgct
    21001 gtgggtctgc cgggtcaaag agttcggccc gctgattgtc tctattgata cccacggcaa
    21061 caacctgata gccgaaaaca aaaagctgtt cgccgaacgc cgcgatccca tcgtggaaga
    21121 gatctgcgag cacgtccatt acatcaaata accctcccgg agaggctcac ccctctcctt
    21181 tttcgcaggc ataacacgat gaaaccttcc cctgaatggt ggcgatatct tgcgccgctg
    21241 gcggtcatcg ccattattgc tctacttccg gttcccgcag ggctggagaa ccatacctgg
    21301 ctctactttg ccgtttttac tggcgtgatc gttggactga tcctcgaacc cgtgccgggt
    21361 gccgtggtgg cgatggtggg tatctccatc atcgccatac tctctccctg gctgctgttc
    21421 agccccgaac agctcgccca gccaggcttt aagttcaccg ccaaatccct ctcctgggca
    21481 gtttctggtt tttctaattc ggttatctgg ctcattttcg ccgcctttat gtttggcaca
    21541 ggctatgaaa aaaccgggct tggacgccgc attgcgctga ttctggtgaa aaagatggga
    21601 catcgcacgc tgtttctcgg ctatgcggtg atgttctccg agctgatcct cgcacctgta
    21661 acaccgtcca actcggcgcg tggtgcgggg attatctatc ccatcatccg taacctgcca
    21721 ccgctctatc aatcacaacc aaacgacagc agttcgcgca gcattggctc gtacatcatg
    21781 tggatgggga ttgttgccga ctgcgtgacc agcgccattt tcttgacggc gatggcacca
    21841 aacttgctgt taattggact gatgaaaagc gcatctcacg ccacgctgag ttggggcgac
    21901 tggttcctcg ggatgttgcc gctcagcatt ttactggttc tgctggttcc ctggctggct
    21961 tacgtgctgt tcccgccggt actgaagtct ggcgatcagg tgccgcgctg ggcagagacg
    22021 gaactgcagg caatgggccc gctctgttcg cgtgaaaaac ggatgctggg gctgatggta
    22081 ggcgcgctgg tgctgtggat tttcggcggt gattatatcg atgccgcgat ggtcggttac
    22141 agcgtggtgg cactgatgct gcttctgcgc attatcagtt gggacgacat tgtcagtaat
    22201 aaagcggcgt ggaacgtttt cttctggctg gcctcgctta tcaccctcgc caccggactc
    22261 aacaacaccg gttttattag ctggtttggc aaactgttag caggcagctt aagcggttat
    22321 tcgccaacga tggtgatggt ggcgttgatt gtggtgtttt atctactgcg ctactttttc
    22381 gccagcgcca cggcgtatac ctccgctctc gcaccgatga tgattgccgc tgcgctggcg
    22441 atgccggaaa tcccgctgcc ggtgttctgc ctgatggttg gtgcggcaat tggtctgggg
    22501 agcattctta caccatacgc caccggcccc agtccgattt actacggcag tggttatctg
    22561 ccaacggcgg attactggcg actgggggcg atttttgggc tgatattcct cgtattgctg
    22621 gtgattaccg gcttactgtg gatgcccgtg gtgttgcttt aagtgaggac ggtgccggaa
    22681 attgctctcc ggcacccaat agttttacgc agccggtaac tccgccagcg gccagcgcgg
    22741 acgcacgcta acgccgagat ccgccgtcgc gcctgcttta aaccgcacca ttccggcata
    22801 ggcgatcatc gcgccgttat cagtacaaaa ttccggacgc gcgtagaaca cttcgccgcg
    22861 gcgttttttc atcatttcag ccagcttcgc ccgtaacgta cggttagcac tcacgccgcc
    22921 cgccatgacc agtcgcttaa agcccgtctg atccagcgcc cgcttgcact taatcatcag
    22981 cgtatcgacc accgcatctt caaaggcgcg ggcgatatca gcacgcgtct ggtcgtcggt
    23041 gccgttgtca cgaatggtat ttgccgcgaa ggttttcagg ccggagaagc tgaaatccag
    23101 ccccggacgg tcggtcatcg gacgcgggaa gacaaagcgc ccggcagtac cctgagccgc
    23161 cattttcgac agtaacggcc cgccaggata atccagcccc agcagcttcg cggttttatc
    23221 aaacgcttcc ccggcggcat catcgataga ctcgccgagc agctcgtact gaccaatgcc
    23281 agtcacgctg attaactgcg tatgaccgcc ggaaacaagc agcgcaacaa acggaaattc
    23341 cggcgggtta tcttccagca tcggcgctaa cagatgccct tccatatggt gtacagggat
    23401 cgccggaacg tcccaggcaa acgccagaga acgccccacg gtcgcgccaa ccagtagcgc
    23461 gccgactaat ccagggcctg cggtataggc cacagcatca atgtcttttg ccgttaaacc
    23521 agactccttt agcgccgcct ggatcaacgg tacggtttta cgcacatgat cgcgggaggc
    23581 cagttcaggc acgacgccgc cgtagtcagc gtgcaatttc acctgactat acaattggtt
    23641 ggctaacaaa cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc aggaagtttc
    23701 aatacccagt acacgcatga cttgttttac ctcgctttat taccgcgcag tgtaggacca
    23761 atgcgggttg atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat actcctcacc
    23821 cttataaaag tccctttcaa aaaaggccgc ggtgctttac aaagcagcag caattgcagt
    23881 aaaattccgc accattttga aataagctgg cgttgatgcc agcggcaaac cgaattaatc
    23941 aaaggtgaga ggcacatgcc ggtaattaaa gtacgtgaaa acgagccgtt cgacgtagct
    24001 ctgcgtcgct tcaagcgttc ctgcgaaaaa gcaggtgttc tggcggaagt tcgtcgtcgt
    24061 gagttctatg aaaaaccgac taccgaacgt aagcgcgcta aagcttctgc agtgaaacgt
    24121 cacgcgaaga aactggctcg cgaaaacgca cgccgcactc gtctgtacta attccccgag
    24181 agcgttgctc tccgatcaga ccgagttgta gttgtaaggc cgtgcttccg aaaggaatgc
    24241 gcggcttatt ttcgtttatg aattgctaaa aatcggggcc tatggctgga cgaatcccac
    24301 gcgtattcat taatgatctg ctggcacgca ctgacatcgt cgatctgatc gatgcccgtg
    24361 tgaagctgaa aaagcagggc aagaatttcc acgcgtgttg tccattccac aacgagaaaa
    24421 ccccgtcctt caccgttaac ggtgagaaac agttttacca ctgctttgga tgtggcgcgc
    24481 acggcaacgc gatcgacttc ctgatgaact acgacaagct cgagttcgtc gaaacggtcg
    24541 aagagctggc agcaatgcac aatcttgaag tgccatttga agcaggcagc ggccccagcc
    24601 agatcgagcg ccatcagcgg caaacgcttt atcagttgat ggacggtctg aatacgtttt
    24661 accaacaatc tttacagcaa cctgttgcca cgtctgcgcg ccagtatctg gaaaaacgcg
    24721 gattaagcca cgaggttatc gctcgctttg cgattggttt tgcgcccccc ggctgggaca
    24781 acgtcctgaa gcggtttggc ggcaatccag aaaatcgcca gtcattgatt gatgcgggca
    24841 tgttggtcac taacgatcag ggacgcagtt acgatcgttt ccgcgagcgg gtgatgttcc
    24901 ccattcgcga taaacgcggt cgggtgattg gttttggcgg gcgtgtgctg ggcaacgata
    24961 cccccaaata cctgaactcg ccggaaacag acattttcca taaaggccgc cagctttacg
    25021 gtctttatga agcgcagcag gataacgctg aacccaatcg tctgcttgtg gtcgaaggct
    25081 atatggacgt ggtggcgctg gcgcaatacg gcattaatta cgccgttgcg tcgttaggta
    25141 cgtcaaccac cgccgatcac atacaactgt tgttccgcgc gaccaacaat gtcatttgct
    25201 gttatgacgg cgaccgtgca ggccgcgatg ccgcctggcg agcgctggaa acggcgctgc
    25261 cttacatgac agacggccgt cagctacgct ttatgttttt gcctgatggc gaagaccctg
    25321 acacgctggt acgaaaagaa ggtaaagaag cgtttgaagc gcggatggag caggcgatgc
    25381 cactctccgc atttctgttt aacagcctga tgccgcaagt tgatctgagt acccctgacg
    25441 ggcgcgcacg tttgagtacg ctggcactgc cattgatatc gcaggtgccg ggcgaaacgc
    25501 tgcgaatata tcttcgtcag gaattaggca acaaattagg catacttgat gacagccagc
    25561 ttgaacgatt aatgccaaaa gcggcagaga gcggcgtttc tcgccctgtt ccgcagctaa
    25621 aacgcacgac catgcgtata cttatagggt tgctggtgca aaatccagaa ttagcgacgc
    25681 tggtcccgcc gcttgagaat ctggatgaaa ataagctccc tggacttggc ttattcagag
    25741 aactggtcaa cacttgtctc tcccagccag gtctgaccac cgggcaactt ttagagcact
    25801 atcgtggtac aaataatgct gccacccttg aaaaactgtc gatgtgggac gatatagcag
    25861 ataagaatat tgctgagcaa accttcaccg actcactcaa ccatatgttt gattcgctgc
    25921 ttgaactgcg ccaggaagag ttaatcgctc gtgagcgcac gcatggttta agcaacgaag
    25981 aacgcctgga gctctggaca ttaaaccagg agctggcgaa aaagtgattt aacggcttaa
    26041 gtgccgaaga gcgatcggga agcccccgac agccgcactg agaggcagcg gcaaatatat
    26101 aagtacgccc tcgtaattat cgttggcggt aaacaaccgt tggatttcag cgttaacggc
    26161 tgaaggacat cgggtcaatc gcccaacacc aacctcatga aataagtgtg gataccgtct
    26221 tatggagcaa aacccgcagt cacagctgaa acttcttgtc acccgtggta aggagcaagg
    26281 ctatctgacc tatgccgagg tcaatgacca tctgccggaa gatatcgtcg attcagatca
    26341 gatcgaagac atcatccaaa tgatcaacga catgggcatt caggtgatgg aagaagcacc
    26401 ggatgccgat gatctgatgc tggctgaaaa caccgcggac gaagatgctg ccgaagccgc
    26461 cgcgcaggtg ctttccagcg tggaatctga aatcgggcgc acgactgacc cggtacgcat
    26521 gtacatgcgt gaaatgggca ccgttgaact gttgacccgc gaaggcgaaa ttgacatcgc
    26581 taagcgtatt gaagacggga tcaaccaggt tcaatgctcc gttgctgaat atccggaagc
    26641 aatcacctat ctgctggaac agtacgatcg tgttgaagca gaagaagcgc gtctgtccga
    26701 tctgatcacc ggctttgttg acccgaacgc agaagaagat ctggcaccta ccgccactca
    26761 cgtcggttct gagctttccc aggaagatct ggacgatgac gaagatgaag acgaagaaga
    26821 tggcgatgac gacagcgccg atgatgacaa cagcatcgac ccggaactgg ctcgcgaaaa
    26881 atttgcggaa ctgcgcgctc agtacgttgt aacgcgtgac accatcaaag cgaaaggtcg
    26941 cagtcacgct gctgctcagg aagagatcct gaaactgtct gaagtattca aacagttccg
    27001 cctggtgccg aagcagtttg actacctggt caacagcatg cgcgtcatga tggaccgcgt
    27061 tcgtacgcaa gaacgtctga tcatgaagct ctgcgttgag cagtgcaaaa tgccgaagaa
    27121 aaacttcatt accctgttta ccggcaacga aaccagcgat acctggttca acgcggcaat
    27181 tgcgatgaac aagccgtggt cggaaaaact gcacgatgtc tctgaagaag tgcatcgcgc
    27241 cctgcagaaa ctgcagcaga ttgaagaaga aaccggcctg accatcgagc aggttaaaga
    27301 tatcaaccgt cgtatgtcca tcggtgaagc gaaagcccgc cgtgcgaaga aagagatggt
    27361 tgaagcgaac ttacgtctgg ttatttctat cgccaagaaa tacaccaacc gtggcttgca
    27421 gttccttgac ctgattcagg aaggcaacat cggtctgatg aaagcggttg ataaattcga
    27481 ataccgccgt ggttacaagt tctccaccta cgcaacctgg tggatccgtc aggcgatcac
    27541 ccgctctatc gcggatcagg cgcgcaccat ccgtattccg gtgcatatga ttgagactat
    27601 caacaaactc aaccgtattt ctcgccagat gctgcaagag atgggccgtg aaccgacgcc
    27661 ggaagaactg gctgaacgta tgctgatgcc ggaagacaag atccgcaaag tgctgaagat
    27721 cgccaaagag ccaatctcca tggaaacgcc gatcggtgat gatgaagatt cgcatctggg
    27781 ggatttcatc gaggatacca ccctcgagct gccgctggat tctgcgacca ccgaaagcct
    27841 gcgtgcggca acgcacgacg tgctggctgg cctgaccgcg cgtgaagcga aagttctgcg
    27901 tatgcgtttc ggtatcgata tgaacaccga ccacacgctg gaagaagtgg gtaaacagtt
    27961 cgacgttacc cgcgaacgta tccgtcagat cgaagcgaag gcgctgcgca aactgcgtca
    28021 cccgagccgt tctgaagtgt tgcgtagctt cctggacgat taatcggtag gccggatcag
    28081 gcgttacgcc gcacccggca ctagtccctc tgcacaaacg ccaccttttc ggtggcgttt
    28141 tttatcgccc acgcactacc agcgcctggt ccagctcgcg atacgcttca accagtttct
    28201 ctagtgaaac gcgacttaaa ccgctgggat ttggcagcac ccaaatctgc gtcgaaccaa
    28261 tggtgagcgt ttgtttcccc cactgtgcac cgcgctggct gaatccctgt tcatatgctt
    28321 gtttgcccag aatcgccaac gcctgcggct gataatcttc aattttttca atcagcttac
    28381 gcccacctgc gtgcagctcc tgctttgaaa cttcattggc ttgcaccgtt ggacggtcta
    28441 ccagtttggt gacgccacaa cgataatcca gcagatgctg tgcctcctgc ggcttcaact
    28501 gacggtcggt aaacccggcc tgatatatca ccttccagaa gcgatttgct ggatgagcaa
    28561 agggaaaacc agtcccggcg gatgaaagcc cagggttgat accgcaaaac acgacccgta
    28621 accctggagc caaaatatcc tcaaccatat ttactccttt ctaacatcac caggaaaagt
    28681 ataacggatt gaaaatacat tgtttataaa aacagcaggc gcgcggtaat ggctggattg
    28741 cgacacggag ttactttata atccgctacc atggcccctt agctcagtgg ttagagcagg
    28801 cgactcataa tcgcttggtc gctggttcaa gtccagcagg ggccaccaga tatagcaaag
    28861 gctgacgaga aatcgtcagc ctttttcttt tacatatcag ttactttgcg tgccagtagg
    28921 ccgctgcacg aacccgctgt gggtcaaact gttccgtttc aaagcggcgg cttaaattct
    28981 taacgacttt gccttcgccg gttatccaga tgaagtaatc atcggcaggg atttgcatct
    29041 gcgccagacg cgcatctacc gcctgctcat catgtgccag ccattcgata ttaaaaccat
    29101 caaggtgcgc gagataatcc tgacaggcgt tatcccgcac gctaaccagc acgctaactt
    29161 gcggtttaac ggcaagtttg ctcaacgttt ccaggcggcg gcgcaatgcg ggcattccgg
    29221 attcatcgca gacatacagc tgatacgcgt aatcttccgg caccaccagc gaaccgcgcg
    29281 gacctgccac cgtgagttta tcgcccggtt gggcctgcat tgcccagcaa ctggcgacgc
    29341 cgtcttcgtg aataaagaaa tcaatcgcca gttcatggcg tagttcgtca tacaacggcg
    29401 tatagtcacg cgacggtggg cgtggtcctt ccggccagac gatgccctct tccgttaccg
    29461 ttggcggcac aaagtgagcg tcaggttgag gaaagaagag tttgctgtga tcgtcaaagc
    29521 cgcgcgatgt aaaaccgtcc agcgcctcgc cgccgaggac aatgcgctga aaaccggcgc
    29581 tgatacgctc aacgcgtagc acagtcagtt cacggaagcg cagatcattg cgaacgcgct
    29641 gcgggtagcg gggggtgtta ttcattgtta tcgccttcgt g
//
LOCUS       DAFNBJ010000051        29491 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000051 DAFNBJ010000000
VERSION     DAFNBJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.051"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..469)
                     /gene="sulA"
                     /locus_tag="L2X49_002081"
     CDS             complement(<1..469)
                     /gene="sulA"
                     /locus_tag="L2X49_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBN4797164.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATR"
     gene            688..1317
                     /gene="sxy"
                     /locus_tag="L2X49_002082"
     CDS             688..1317
                     /gene="sxy"
                     /locus_tag="L2X49_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBN4797165.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(1280..3442)
                     /gene="yccS"
                     /locus_tag="L2X49_002083"
     CDS             complement(1280..3442)
                     /gene="yccS"
                     /locus_tag="L2X49_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBN4797166.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(3452..3898)
                     /locus_tag="L2X49_002084"
     CDS             complement(3452..3898)
                     /locus_tag="L2X49_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBN4797167.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            4021..6075
                     /gene="helD"
                     /locus_tag="L2X49_002085"
     CDS             4021..6075
                     /gene="helD"
                     /locus_tag="L2X49_002085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBN4797168.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(6107..6565)
                     /gene="mgsA"
                     /locus_tag="L2X49_002086"
     CDS             complement(6107..6565)
                     /gene="mgsA"
                     /locus_tag="L2X49_002086"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBN4797169.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6661..7323)
                     /locus_tag="L2X49_002087"
     CDS             complement(6661..7323)
                     /locus_tag="L2X49_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBN4797170.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7496..7909
                     /locus_tag="L2X49_002088"
     CDS             7496..7909
                     /locus_tag="L2X49_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBN4797171.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(7954..8271)
                     /gene="hspQ"
                     /locus_tag="L2X49_002089"
     CDS             complement(7954..8271)
                     /gene="hspQ"
                     /locus_tag="L2X49_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBN4797172.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(8329..9519)
                     /gene="rlmI"
                     /locus_tag="L2X49_002090"
     CDS             complement(8329..9519)
                     /gene="rlmI"
                     /locus_tag="L2X49_002090"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBN4797173.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            9614..9892
                     /gene="yccX"
                     /locus_tag="L2X49_002091"
     CDS             9614..9892
                     /gene="yccX"
                     /locus_tag="L2X49_002091"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBN4797174.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            complement(9889..10218)
                     /gene="tusE"
                     /locus_tag="L2X49_002092"
     CDS             complement(9889..10218)
                     /gene="tusE"
                     /locus_tag="L2X49_002092"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBN4797175.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(10309..10968)
                     /gene="yccA"
                     /locus_tag="L2X49_002093"
     CDS             complement(10309..10968)
                     /gene="yccA"
                     /locus_tag="L2X49_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBN4797176.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(11175..11262)
                     /locus_tag="L2X49_002094"
     tRNA            complement(11175..11262)
                     /locus_tag="L2X49_002094"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11226..11228),aa:Ser,seq:tga)
     gene            11689..12807
                     /gene="hyaA"
                     /locus_tag="L2X49_002095"
     CDS             11689..12807
                     /gene="hyaA"
                     /locus_tag="L2X49_002095"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBN4797177.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGNQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            12804..14597
                     /gene="hyaB"
                     /locus_tag="L2X49_002096"
     CDS             12804..14597
                     /gene="hyaB"
                     /locus_tag="L2X49_002096"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBN4797178.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            14616..15323
                     /gene="hyaC"
                     /locus_tag="L2X49_002097"
     CDS             14616..15323
                     /gene="hyaC"
                     /locus_tag="L2X49_002097"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBN4797179.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            15320..15907
                     /locus_tag="L2X49_002098"
     CDS             15320..15907
                     /locus_tag="L2X49_002098"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBN4797180.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            15904..16302
                     /gene="hyaE"
                     /locus_tag="L2X49_002099"
     CDS             15904..16302
                     /gene="hyaE"
                     /locus_tag="L2X49_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBN4797181.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            16299..17156
                     /locus_tag="L2X49_002100"
     CDS             16299..17156
                     /locus_tag="L2X49_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBN4797182.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            17290..18834
                     /gene="appC"
                     /locus_tag="L2X49_002101"
     CDS             17290..18834
                     /gene="appC"
                     /locus_tag="L2X49_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBN4797183.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            18846..19982
                     /gene="appB"
                     /locus_tag="L2X49_002102"
     CDS             18846..19982
                     /gene="appB"
                     /locus_tag="L2X49_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBN4797184.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            19995..20087
                     /gene="cbdX"
                     /locus_tag="L2X49_002103"
     CDS             19995..20087
                     /gene="cbdX"
                     /locus_tag="L2X49_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBN4797185.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            20167..21465
                     /gene="appA"
                     /locus_tag="L2X49_002104"
     CDS             20167..21465
                     /gene="appA"
                     /locus_tag="L2X49_002104"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBN4797186.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGAIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(21585..23765)
                     /gene="etk"
                     /locus_tag="L2X49_002105"
     CDS             complement(21585..23765)
                     /gene="etk"
                     /locus_tag="L2X49_002105"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBN4797187.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGINVYATIPMSEWLDKRARLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(23785..24231)
                     /gene="etp"
                     /locus_tag="L2X49_002106"
                     /gene_synonym="yccY"
     CDS             complement(23785..24231)
                     /gene="etp"
                     /locus_tag="L2X49_002106"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBN4797188.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(24219..25358)
                     /locus_tag="L2X49_002107"
     CDS             complement(24219..25358)
                     /locus_tag="L2X49_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBN4797189.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(25404..27500)
                     /locus_tag="L2X49_002108"
     CDS             complement(25404..27500)
                     /locus_tag="L2X49_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBN4797190.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGHWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVLWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(27500..28246)
                     /locus_tag="L2X49_002109"
     CDS             complement(27500..28246)
                     /locus_tag="L2X49_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBN4797191.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(28243..28887)
                     /gene="gfcB"
                     /locus_tag="L2X49_002110"
     CDS             complement(28243..28887)
                     /gene="gfcB"
                     /locus_tag="L2X49_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HBN4797192.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(28994..29299)
                     /locus_tag="L2X49_002111"
     CDS             complement(28994..29299)
                     /locus_tag="L2X49_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797193.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT         7116 a         7518 c         7781 g         7076 t
ORIGIN      
        1 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
       61 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
      121 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
      181 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
      241 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
      301 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
      361 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
      421 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
      481 cccctgtgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
      541 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
      601 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
      661 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
      721 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
      781 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
      841 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
      901 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
      961 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
     1021 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
     1081 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
     1141 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
     1201 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
     1261 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
     1321 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
     1381 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
     1441 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
     1501 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
     1561 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
     1621 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
     1681 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
     1741 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
     1801 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
     1861 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
     1921 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
     1981 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
     2041 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
     2101 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
     2161 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatttga atgatggcgt
     2221 agccgaagca caacaccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
     2281 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
     2341 gctcattttc atcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
     2401 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
     2461 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
     2521 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
     2581 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
     2641 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
     2701 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
     2761 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
     2821 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
     2881 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
     2941 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
     3001 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
     3061 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
     3121 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
     3181 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
     3241 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
     3301 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
     3361 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
     3421 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
     3481 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
     3541 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
     3601 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
     3661 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
     3721 cgacatgaat agcttcattg ccataaggca ccagagacag tttagtgatc tcccagcagg
     3781 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
     3841 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
     3901 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
     3961 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
     4021 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
     4081 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
     4141 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
     4201 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
     4261 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
     4321 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
     4381 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
     4441 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
     4501 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
     4561 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
     4621 caggcccggg cagttgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
     4681 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
     4741 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgt
     4801 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
     4861 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
     4921 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
     4981 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
     5041 aacttctggg atgatgaaaa attacagcgt cgtcttgcct cacgcctcga tcgttgggta
     5101 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
     5161 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
     5221 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
     5281 attctggaga aaggccgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
     5341 gatatctcgc cgcagcgcgc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
     5401 acgttgttcg ccgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
     5461 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg atcgctgtga tttagacacg
     5521 acttaccgtt ttaacagtcg tatcggtgag gtggcaaacc ggtttattca gcagaaccca
     5581 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
     5641 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
     5701 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
     5761 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
     5821 caggcggatt acgtcatcat cgttggcttg caggagggga gtgatggttt tccggctgcg
     5881 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
     5941 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
     6001 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggta
     6061 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
     6121 ccgcgagata acgctgataa tcggggatca gaatatcgac cgcgtcgttg aaatgcggcg
     6181 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
     6241 ccagacgcaa caaggctttc acgtcaggat cgtgcggcac ggcatttagt gggtcccaga
     6301 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
     6361 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
     6421 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
     6481 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
     6541 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
     6601 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
     6661 tcaggaagat ggttgttttt ccgcccattg taggaaagta tttcgcgttt gtgggtcagc
     6721 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
     6781 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
     6841 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
     6901 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
     6961 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
     7021 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
     7081 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
     7141 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
     7201 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
     7261 ggtggcaaaa acggataccg gcaaacataa tgcaatcaag gtggtcacga tgccggtttt
     7321 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
     7381 catacttact aacatataga catatttccc gcttttgctc tcaatcattc gtattagctg
     7441 catggttggc atgtcgactt tgtcgtacac tttgttaaac agtctggagg aaataatgaa
     7501 agaaaccgat attgcaggta ttttaacgtc cactcacacc attgcgctgg tgggggcaag
     7561 cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc aggggtatca
     7621 cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc aggggtatgg
     7681 cacgctggca gacgttccgg aaaaagtcga tatggtcgat gttttccgta attcagaagc
     7741 ggcgtggggc gtggcgcagg aagctatcgc cattggcgcg aaaaccctct ggatgcaact
     7801 gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttaa atgtggtgat
     7861 ggatcgctgc ccggctattg agatccctcg cctgggcctg gccaaataaa aaatccccgg
     7921 aaggcaaaaa ccttccgggg attcgttcag ggatcagtta cgcagacgcg gggcctggag
     7981 ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat gttcatcctg
     8041 caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc cgttatcgtc
     8101 ctccatcacc acgtgatacc acggagcggc gcgaagctcg tcattcaccg ccagctcatc
     8161 aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc cgagataacc
     8221 taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca tagtcacctc
     8281 ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt acatgacgcg
     8341 acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg gatgatcggc
     8401 tgcctgacgg aactgctcta taaattgtac atcacggccg gcatcaattg cggcatccgc
     8461 gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag tcaggagaat
     8521 accgccttca ttcagcaact gaatcgccag catgttgata tctttatagc cacgacacgc
     8581 gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga taacgtcaaa
     8641 tttttcaccg cgatcgcgat aagtacgcag caatttaaag acatcatcac ggacaaactc
     8701 agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc
     8761 ctgggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg atacggcgaa
     8821 accaccggta taggagaaac agttcagcac acgtttattt tcaacgtagc ggcgggtagc
     8881 caggcggctg tctcgctggt ccaggtagta gcccgttttg tgtccgtgct gaatatccac
     8941 cagcagtttc atgccgtgtt cttcaatcgg cagcagggca ggtggcaact cgccggtgac
     9001 gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta
     9061 aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc gctgatattc
     9121 tgcgccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga tagtaatacc
     9181 cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat ctttttgcgc
     9241 cagccagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat
     9301 agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg gcgaataagc
     9361 gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac cgaggctggc
     9421 tttaccttcc atgcgggcaa cggccccgga aaagacccac ggatggcgac gaagtaatga
     9481 tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt ctgtcgatgc
     9541 aaagaaatgg gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc cattaaggat
     9601 aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc gagttcaggg
     9661 cgtaggattt cgctacacca cacagtatga agcgaaaaga ctgggtttaa ccgggtacgc
     9721 caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtgga
     9781 aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtgg aacgggtgct
     9841 tagcgagccg catcatccct cgggggaatt agccgatttt cgtatccgtt aaatacattt
     9901 taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt tcggaaacag
     9961 tcgatataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca tcgcttttac
    10021 cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca caaaacgcac
    10081 cacttcccag tgttctggcg acagcgaaat cccttcgttc tctgcaatca ccacagccag
    10141 cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt ctatctcttt
    10201 accttcgaag atcagcataa ttattactac cttaatcaga ctggcggcag tgtaacaaaa
    10261 aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt aatcgcggct
    10321 agcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa catacaggct
    10381 aaccgtggca cgaatatagt tcgtctcacc gccatgaatg atgttgctgg tttcaaacaa
    10441 gatagcgcca gaggagatca gaatgaagac cgcgctgatc gccagatgca gagcaggcag
    10501 ttgcaggaag atattagcaa ccataccaat cagcaccacc acaatacccg ccatcagcat
    10561 accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa
    10621 cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg cagacagata
    10681 ggtgttcaga ataggtccga ggatataacc cagaaaaccg gtaaaggcga atgcggagat
    10741 aatcccggtc ggcttattcg ccgttttata ggtcaggaac atcaaaccat acatacccac
    10801 cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag ttgcggtaat
    10861 cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca ccttatgggt
    10921 gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg agtcactctc
    10981 tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac ccagaagttt
    11041 tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca ggtcattaag
    11101 caaatataac gccctgagaa tttcgacagg caaaagaaaa aggggttagc atttagctaa
    11161 ccccttatct tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
    11221 ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac gcgcactata
    11281 aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa
    11341 gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga attccttctc
    11401 ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggccaaaaga aaaataggtc
    11461 catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca aaagtttcac
    11521 tacgctttat taacaatact ttctggcgac gtgtgccagt gcagaaggat gagctttcgt
    11581 tttcagcatc tcacgtgaag cgatggtttg ccttgctaca gggacgtcgc ttgccgacca
    11641 taagcgcccg gtgtcctgcc ggtgttgcta ggagaagaga cgtgcgatat gaataacgag
    11701 gaaacatttt accaggccat gcggcgtcag ggcgttaccc ggcgcagctt tctcaaatat
    11761 tgtagtctgg ctgccacgtc gctgggatta ggcgcgggaa tggcaccaaa gattgcctgg
    11821 gcgctggaga acaaaccgcg cattccggtg gtatggatcc acggtctgga atgcacctgc
    11881 tgtaccgaat cttttatccg ctccgctcac ccactggcga aggacgtcat cctttccctt
    11941 atttctctcg attacgacga tactttgatg gctgccgccg gaaaccaggc ggaagaagtc
    12001 tttgaagaca tcatcacgca atacaatggc aaatatatcc tcgcagtaga aggcaatccg
    12061 ccgctgggcg agcaggggat gttctgtatc agcagcggtc gaccgtttat tgagaaactc
    12121 aaacgtgccg ctgccggagc cagcgcgatt atcgcctggg gaacctgcgc gtcctggggc
    12181 tgcgtgcagg ccgcgcggcc caatccgacg caggcaacgc ctatcgacaa agtcatcacc
    12241 gacaaaccca ttatcaaagt acctggctgc ccgccgatcc cggatgtgat gagcgccatc
    12301 attacttaca tggtgacctt tgatcgcttg ccagatgtcg acagaatggg ccgtccactg
    12361 atgttttatg gtcagcgtat ccatgataaa tgctatcgcc gcgctcactt cgacgccgga
    12421 gagttcgtcc agagttggga tgatgacgct gcccgcaaag gttactgcct gtacaaaatg
    12481 ggctgcaaag ggcctaccac ctataacgcc tgttcctcca cacgctggaa tgatggcgtt
    12541 tcttttccaa tccagtctgg tcacggctgc ctgggctgtg cggaaaatgg tttctgggat
    12601 cgcggttcgt tctacagccg cgtggtcgat attccgcaaa tgggtactca ttccactgcc
    12661 gataccgtcg gtttaaccgc gcttggcgtg gtggcagcgg ctgttggtgt gcacgcagtc
    12721 gccagcgccg ttgaccagcg cagacgtcat aaccagcaac ctacagaaac cgaacatcag
    12781 ccaggcaatg aggataaaca ggcatgagca ctcagtacga aactcaggga tacaccatca
    12841 ataatgccgg acgccgcctg gtggtcgacc cgattacgcg catcgaaggc cacatgcgct
    12901 gcgaagtgaa tattaacgat cagaatgtga tcaccaatgc cgtctcctgc ggcaccatgt
    12961 ttcgcgggct ggagatcatt ctgcaagggc gcgacccgcg cgatgcgtgg gcgttcgttg
    13021 aacgtatctg cggcgtctgt actggcgtac acgccctggc ttcggtttac gccatcgaag
    13081 atgctatcgg tattaaagtg ccggacaacg ccaatatcat ccgcaacatt atgctggcaa
    13141 cgctctggtg ccacgatcat ctggtgcact tctatcagct tgccgggatg gactggatcg
    13201 atgtgttaga tgcgctgaaa gccgacccgc ggaaaacctc cgaactggcg caaagtctct
    13261 cctcttggcc gaaatcatcc cctggctatt tcttcgacgt acaaaaccgc ctgaaaaaat
    13321 ttgttgaagg cgggcagttg gggatcttcc gcaatggcta ctgggggcac ccgcagtaca
    13381 aactgccgcc agaagctaac ctgatgggct ttgcccacta tctcgaagct ctcgatttcc
    13441 agcgtgaaat tgtcaaaatc cacgcggtct ttggcggtaa aaacccgcat ccaaactgga
    13501 ttgtcggcgg gatgccttgc gccatcaaca ttgacgaaag cggcgcggtc ggggcagtca
    13561 atatggaacg cctgaacctg gtgcagtcaa ttatcacccg cacggcggac ttcattaaca
    13621 acgtgatgat ccccgacgcc ttagccatcg gtcagttcaa caaaccgtgg agcgaaatcg
    13681 gcacgggtct ttctgataaa tgtgtcctca gctacggggc attcccggat attgccaacg
    13741 actttggtga gaaaagtctg ctgatgcctg gcggcgcggt gattaacggc gatttcaaca
    13801 atgtgctgcc agtggatttg gttgatccgc agcaggtgca ggagtttgtc gatcacgcct
    13861 ggtatcgtta tcccaacgat caggtcgggc gtcatccgtt tgatggcatc accgacccgt
    13921 ggtacaaccc cggcgatgtc aaaggcagcg ataccaacat tcagcagctg aatgaacagg
    13981 aacgctactc gtggatcaaa gcgccacgct ggcgcggtaa cgcgatggaa gtggggccgc
    14041 tggcgcgcac gttaatcgct tatcacaaag gcgatgctgc gaccgttgag tcggtcgatc
    14101 gcatgatgtc ggcgttgaac ctgccgcttt ccggtatcca gtcaacgtta ggccgcattt
    14161 tgtgccgcgc gcacgaagcg cagtgggccg caggtaagtt gcagtatttc ttcgacaagc
    14221 tgatgaccaa cctgaaaaac ggcaatctcg ccactgcttc cacggaaaaa tgggaacctg
    14281 caacctggcc gacagagtgc cgtggtgtcg gttttaccga agcgccgcgc ggggcgttag
    14341 gccactgggc cgccattcgc gatggcaaga ttgatctcta ccagtgcgtg gtgccgacca
    14401 cctggaacgc cagcccgcgc gatcccaaag ggcagattgg cgcttatgaa gcggcgctga
    14461 tgaacaccaa aatggcgatc cccgagcaac cgctggagat cctgcgtact ctgcacagct
    14521 ttgacccgtg cctcgcctgt tcaacacacg tgctgggcga cgacggtagc gagctgatct
    14581 ccgtgcaggt gcgttaacag cgaaggagaa tcatcatgca acagaaaagc gacaacgttg
    14641 tcagccacta tgtctttgaa gcgccagtcc gcatctggca ctggttgacg gtgttatgca
    14701 tggcggtgtt gatggtcacc ggatacttta tcggcaagcc gctaccttcc gtcagcggcg
    14761 aggcgacgta tctgttctat atgggctaca tcaggttaat tcacttcagc gccgggatgg
    14821 tttttaccgt ggttttgctg atgcggatct actgggcttt tgttggcaat cgatactccc
    14881 gcgagctgtt tatcgtgccg gtatggcgta aaagctggtg gcagggcgtg tggtatgaaa
    14941 tccgctggta tctgtttctg gcaaaacgtc cgagtgccga tataggccat aatcccatcg
    15001 cccaggcggc gatgttcggc tatttcctga tgtcggtctt tatgatcatc actggttttg
    15061 cgctgtacag cgaacacagc cagtacgcta tttttgcgcc gttccgttat gtggtggaat
    15121 ttttctactg gacgggtggc aactcaatgg acattcacag ctggcatcgg ctggggatgt
    15181 ggctgattgg cgcgtttgtg atcggtcatg tctacatggc gctgcgtgaa gacatcatgt
    15241 ccgacgacac ggtgatctcc accatggtca acggctaccg tagccacaaa tttggcaaaa
    15301 taagtaacaa ggagcgttca tgagcgagca acgcgtggtg gtcatggggc tgggcaacct
    15361 gctgtgggcc gatgaaggct tcggcgtgcg ggtggcggaa cggctgtatg cccattacca
    15421 ctggcccgag tatgtggaga ttgtcgatgg cggtactcag ggactgaact tgctggggta
    15481 tgtcgaaagc gccagccatc tgttgattct cgatgccatt gactacgggc tggaacctgg
    15541 aacgctgcga acctatgccg gagaacgcat tccggcttat ctcagcgcga agaaaatgag
    15601 cctgcatcag aacagtttct ccgaagtgtt ggcgctggcg gatatccgcg gacatctgcc
    15661 agcacatatt gccctcgtcg gtctgcaacc cgcaatgctc gacgactacg gcggtagcct
    15721 gagcgaactg gcacgggagc aactgcccgc tgcggaacag gcggcgctgg cgcagctggc
    15781 tgcgtgggga attgtgccgc aaccggctaa tgaatcgcgc tgtctcaatt atgactgtct
    15841 gtcgatggaa aattacgaag gcgttcgctt gcgccagtac cggatgacac aggaggagca
    15901 gggatgagca acgacacgcc atttgatgcg ttgtggcaac gaatgctggc gcgcggctgg
    15961 acgccagtca gtgaatcccg tcttgacgac tggcttacgc aagcgccaga cggcgtggtg
    16021 ttattaagca gtgacccgaa acgcacgcca gaggtcagcg ataatccggt aatgattggc
    16081 gaattactgc acgagtttcc cgactataca tggcaggtgg cgattgctga ccttgagcag
    16141 agcgaagcca tcggcgatcg ctttggcgtc tttcgctttc ctgccacttt agtgtttacc
    16201 ggcggaaact atcgcggcgt gctgaatggt attcacccgt gggcggaact gataaacctg
    16261 atgcgcgggc ttgtcgaacc gcagcaggag cgtgcctcat gagcgaaact tttttccatc
    16321 tgctggggcc aggaacgcaa ccgaacgatg acagtttcag catgaatcca ctgccgatca
    16381 cctgtcaggt gaatgatgaa ccgagtatgg cggccctgga gcaatgtgct cacagcccgc
    16441 aggtgattgc gctgttaaac gagttacaac atcaactaag cgaacgccaa ccgccgttgg
    16501 gcgaggtgct ggcagtcgat ctgttaaatc tcaacgccga cgatcgtcac tttatcaata
    16561 cgcttctcgg ggaaggggaa gtgtcagtgc gcattcagca ggctgacgac agtgaaagtg
    16621 aaatacagga ggcgatcttc tgcggattat ggcgggtgcg cagacgtcgc ggcgaaaagt
    16681 tgctggagga caaactggag gctggctgcg cgccgctggc gttgtggcag gcggcaacgc
    16741 aaaatctctt gccgacagat tcgctgttac cgccgcccat tgatggcctg atgaatggcc
    16801 taccgttggc gcatgagtta ctggcacatg tacgtaaccc cgacgcgcag ccgcacagca
    16861 ttaatctgac gcaattaccc atcagcgagg ctgatcggct ttttctctca cgtctctgtg
    16921 ggccgggaaa tattcagatt cgtaccattg gctatggcga gagctatatc aacgccacgg
    16981 ggttacgcca tgtctggcat ttacgctgta cggacacctt aaaaggcccg ttactggaaa
    17041 gttatgaaat ctgcccaata ccggaagtgg tgctggcagc gccagaagat ttggtcgact
    17101 ctgcgcagcg gcttagcgag gtatgtcagt ggctggcgga agctgcaccg acgtaaaaag
    17161 acggtaagta tcgctttcag tcttatgaat atcgcaatcg gcgaatacct ctggtcgtag
    17221 agtttcagga taaagaggga gatctaccat tatcgggtta tttttctctc ttcgcctaca
    17281 ggagtgcgca tgtgggatgt cattgattta tcgcgctggc agtttgctct gaccgcgctg
    17341 tatcactttt tatttgtacc ccttaccctg gggctgattt ttttgctggc tatcatggaa
    17401 accatttacg tggtcaccgg caaaacaatc taccgcgata tgacgcgctt ctggggtaag
    17461 ctcttcggta tcaattttgc tcttggcgtg gctaccggcc tgaccatgga gtttcagttt
    17521 ggtactaact ggtcattcta ttccaactat gtgggcgata tttttggcgc accactggcg
    17581 atggaagcat taatggcctt cttcctcgaa tccacctttg tcgggctgtt cttcttcggc
    17641 tggcaacggc tgaataaata ccagcacctg ctggtgacgt ggctggtggc gttcggttca
    17701 aatctctctg cgttgtggat attgaatgcc aatggttgga tgcaataccc gaccggtgcg
    17761 cattttgata tcgacaccct gcgtatggag atgaccagct tcagcgagct ggtctttaat
    17821 ccggtcagcc aggtgaaatt tgtgcacacc gtaatggcgg gctacgtgac cggggccatg
    17881 tttattatgg cgatcagcgc ctggtattta ctgcgcggac gggagcgcga tgtcgcatta
    17941 cgctcgtttg ccatcggttc cgtcttcggt actctggcga ttatcggtac cctgcaactc
    18001 ggagacagtt ctgcgtatga agtcgcgcaa gtacaaccgg taaaactggc ggcgatggaa
    18061 ggggagtggc aaacggaacc tgcacctgca ccgttccatg tggttgcctg gccggaacag
    18121 gatcaagagc gtaacgcctt tgccctcaaa attcccgcgc tgctagggat cctcgccact
    18181 cactcattag ataaacccgt gccgggtctg aagaatttga tggctgaaac ctacccacgc
    18241 ttgcaacgcg gacgtatggc ctggctgtta atgcaggaaa tatcgcaagg caatcgtgag
    18301 ccgcatgtgt tgcaggcatt ccgggaactg gaaggtgacc tgggctacgg catgttgctc
    18361 tcccgctatg cgccggatat gaatcatgtc acagccgcac agtaccaggc ggcgatgcgt
    18421 ggcgcgatac ctcaggttgc gccggtattc tggagtttcc gcatcatggt gggctgtggt
    18481 tccctgctgc tactggtgat gctgattgcg cttgtccaga cgctgcgtgg caaaatcgac
    18541 cagcatcgct gggtgctgaa aatggcgctc tggagtttgc cgttgccgtg gattgcgatt
    18601 gaagccgggt ggtttatgac cgagtttggt cgtcagccgt gggcgataca ggacatctta
    18661 ccgacatact ccgcgcactc cgctttaacc acaggacaac tggctttctc actgatcatg
    18721 atcgtagggc tttacaccct gttcttaatc gccgaagtct acctgatgca gaaatatgcc
    18781 cgtctggggc cgagcgcgat gcagagtgaa caaccgacgc agcaacaggg gtaaaggaga
    18841 aaatcatgtt tgattatgaa acattgcgct tcatctggtg gctgctgatt ggcgtgatcc
    18901 tggtggtctt tatgatctcc gacggatttg acatggggat tggctgtttg ctgccgctgg
    18961 tggcgcgtga tgatgatgaa cgccggatag tgattaatag cgttggtgcg cactgggaag
    19021 gcaatcaggt ctggttgatc ctcgcaggtg gggcattatt tgccgcctgg cccagagtgt
    19081 atgcagcggc gttttccggc ttttatgtgg cgatgatcct ggtgctgtgc tcgctgttct
    19141 tccgcccgct ggcctttgat tatcgcggaa aaatcgccga tgcacgctgg cgtaaaatgt
    19201 gggatgccgg tctggtcatc ggcagtctgg tgccgccggt agtcttcggt atcgccttcg
    19261 gcaacttgtt gctcggcgtg ccgtttgcct tcacaccgca attacgcgtg gagtatctcg
    19321 gcagcttctg gcaactgctg acgccattcc ctttattgtg cggattgctc agccttggga
    19381 tggtgatttt gcaaggtggc gtctggttac aactgaaaac tgttggtgtg attcatctgc
    19441 gttcacagct ggcgactaaa cgcgctgcac tgttggtgat gctgtgcttt ttgctggcgg
    19501 gttactggct gtgggtcggt attgatggct ttgtactgct cgcccaggat gctaacggtc
    19561 cttccaatcc gttaatgaaa ctggtggcag tgctacctgg tgcctggatg aataattttg
    19621 tcgagtcgcc cgttttgtgg atcttcccgc tgctggggtt cttctgccca ttgctgacgg
    19681 tgatggcgat ttatcgtggt cgcccgggtt ggggattttt gatggcgtca ttgatgcaat
    19741 ttggcgtgat tttcacggca ggcatcacgc tgttcccctt tgtcatgccg tcaagcgtga
    19801 gtccaatctc cagcctgacg ttgtgggaca gcacttccag tcagctgacg ctgagcatta
    19861 tgttggtaat cgtgctgata tttttgccta ttgtgttgct ctacactctc tggagctact
    19921 acaaaatgtg gggacgcatg acgacagaaa ctctccgccg taacgaaaac gagctgtact
    19981 aaggaacagg aacaatgtgg tatttacttt ggttcgtcgg cattttgttg atgtgttcgc
    20041 tctccaccct tgtgttggta tggctggacc cgcgtctgaa aagttaacga acgtaggcct
    20101 gatgcggcgc attagcatcg catcaggcaa tcaataatgt cagatatgaa aagcggaaac
    20161 atatcgatga aagcgatctt aatcccattt ttatctcttc tgattccgtt aaccccgcaa
    20221 tctgcattcg ctcagagtga gccggagctg aagctggaaa gtgtggtgat tgtcagtcgt
    20281 catggtgtgc gtgctccaac caaggccacg caactgatgc aggatgtcac cccagacgca
    20341 tggccaacct ggccggtaaa actgggttgg ctgacaccgc gcggtggtga gctaatcgcc
    20401 tatctcggac attaccaacg ccagcgtctg gtagccgacg gattgctggc gaaaaagggc
    20461 tgcccgcagt ctggtcaggt cgcgattatt gctgatgtcg acgagcgtac ccgtaaaaca
    20521 ggcgaagcct tcgccgccgg gctggcacct gactgtgcaa taaccgtaca tacccaggca
    20581 gatacgtcca gtcccgatcc gttatttaat cctctaaaaa ctggcgtttg ccaactggat
    20641 aacgcgaacg tgactgacgc gatcctcagc agggcaggag gggcaattgc tgactttacc
    20701 gggcatcggc aaacggcgtt tcgcgaactg gaacgggtgc ttaattttcc gcaatcaaac
    20761 ttgtgcctta aacgtgagaa acaggacgaa agctgttcat taacgcaggc attaccatcg
    20821 gaactcaagg tgagcgccga caatgtctca ttaaccggtg cggtaagcct cgcatcaatg
    20881 ctgacggaga tatttctcct gcaacaagca cagggaatgc cggagccggg gtggggaagg
    20941 atcaccgatt cacaccagtg gaacaccttg ctaagtttgc ataacgcgca attttatttg
    21001 ctacaacgca cgccagaggt tgcccgcagc cgcgccaccc cgttattaga tttgatcaag
    21061 acagcgttga cgccccatcc accgcaaaaa caggcgtatg gtgtgacatt acccacttca
    21121 gtgctgttta tcgccggaca cgatactaat ctggcaaatc tcggcggcgc actggagctc
    21181 aactggacgc ttcccggtca gccggataac acgccgccag gtggtgaact ggtgtttgaa
    21241 cgctggcgtc ggcttagcga taacagccag tggattcagg tttcgctggt cttccagact
    21301 ttacagcaga tgcgtgataa aacgccgcta tcattaaata cgccgcccgg agaggtgaaa
    21361 ctgaccctgg caggatgtga agagcgaaat gcgcagggca tgtgttcgtt ggcaggtttt
    21421 acgcaaatcg tgaatgaagc acgcataccg gcgtgcagtt tgtaatgcat aaaaaagagc
    21481 attcagttac ctgaatgctc tgaggctgat gacaaacgaa gaactgtcta atgcgtagac
    21541 cggaagaggc gttcacgccg catccggtca ctttaccttc agttttactc tttctcggag
    21601 taactataac cgtaatagtt atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca
    21661 ccattgagga ttgcgccttt aatattaacg ccagcctgtt ccagacgctg cattgacaag
    21721 ctcacctctt tggcggtgtt caagccaaaa cgcgcaacca gcaggctggt gccgacagag
    21781 cgtcccacga ccgcagcatc gctcaccgcc agcatcggcg gcgtatcgac aatcaccaga
    21841 tcgtaatggt cgttcgccca ttccagtaat tgacgcatcc gatcgcgcat cagcagttca
    21901 gacgggttag gtggcacctg accgcgagta atcacatcaa agcctccttt gccaaaatgc
    21961 tggatcactt tgttgagctc atctttacct gccagatatt ccgacaagcc atgttcatta
    22021 ctcacggtaa acaggttatg cgaataacca cggcgtaagt cggcatcaat aaataacact
    22081 ttttgatcgg actgggcgat caccgctgcc agagttgaac tgacaaacgt tttaccactg
    22141 tctggcgtcg caccggtgat catcagaatg ttattctccg tctccatcat agcgaagtgc
    22201 agactggttc gtagcgcacg tacggcttcc acagcagaat ccgccgggtt atccaccgcc
    22261 aggaagggga tatttttagt acgatggcgc tgctgattag aaaataaatt tttcttacgc
    22321 agacgggcgc gtttatccag ccactcggac attgggatag tggcataaac gttgatgccg
    22381 tgctcttcca gttgttccgg tgcttctaca ccacgacgca acatcgctcg cgccagcacg
    22441 gcacccaccg aaataaacag gccaagaata aaaccaagca ccacgttcaa cgcttttttc
    22501 ggtttcactg gctgcggctg agtgactgcc gggtcgataa tccgcacgtt accaatggca
    22561 ctggatttcg aaatactcaa ctcctgctgg cggttaagta attgcagata taccgcacgg
    22621 cccgcttcta cgtcacgact taaacgcaac acttcctgtt gggtggaagg catagccgat
    22681 acccgcttat tcaggcgttt gcgttcttgc tccagcgtct ggcgtttttc cagcagcgca
    22741 cgataagttg ggtgatcttt cttatacagc tgggagatct ctgcctcgcg gaaagtcagc
    22801 tcattgagtt gattatcaac gttcacaatc tgctcaagaa cggctttggc ttccaggtta
    22861 aggtcaaccg aatcgcgctg ctggcgataa acgttgagtt tttcttccgc ctggtccagc
    22921 tcgctgcgca cttcaggcaa ctggcgctgt aagaattcaa ggctttgtga atcctgcgcc
    22981 gcctggcgag cgatattctg ttgcaaatag ttgttagcga tgctgttcag aatacgagta
    23041 atcaactggg gatcatcacc ggtcatggta agttccagca tcccactttc tttactgcgt
    23101 tcgctaacgg taaaggtttc ctgcaacgcg ttaatcgctt ccagttcggt acgctggctc
    23161 aggacaaact gtgttcctgg tttggcctta atatccgcga tagtcagcgc aacgccatct
    23221 ttttccagac gctggccgac cataccattg acggtgaact cttcaccttc cagcgtatag
    23281 tggccgtttt ccccaaccgt gagtgtcagt tgctgatcct gaccattcag ttgtggaata
    23341 tgcatccagc tgatcgccag ctcacctggt ttctctttgg ttaatctcgc ccagccgcga
    23401 cccacaatcg gaaaatactt ctgctcaacc atgtcgcgca gattcagttc agcaatggtt
    23461 ttaccgagaa tcatgcgcga ttgcagcagt tggatctccg gtgcagactc gggcgatgag
    23521 ttagggatca tatcgctcag gccactgaga atggcgttgc cctgtttctg ctcaacctgg
    23581 accagagtat ctgcctgata aattggcgtg cttaacagcg agtaagcgac agcgatcagc
    23641 gtgaataacg cggtcacgct gataataaac ttacggtgat cccataactc gccgaccaga
    23701 cgaagcagat cgatctcatt ttcctgagtg ctgcctggtg gcgtattcat atttttagtt
    23761 gtcatcgtat tttataaact caaattaccg gctgaggcgc ttcgcccatt cctgactggc
    23821 gcgctccaac ataccgtaga catgttcaaa tgcgtcctga cttttacgat agggatccgg
    23881 gatctctttc tgttccagcc attgcccaaa cagcattgtc ttcccgcgga cctcgggagc
    23941 gattgcggta acctgagcaa tatgttccga ctccattgcc agaatcagat cgtaatttcg
    24001 tgccatctca gcagtgagct tacgtccggc atgtccttca agagatacgc catgattagc
    24061 ggcaacatct gccgctgtcg catctgcagg gtgttttacc agaccatgaa cacctgccga
    24121 tttgactttt acgcccggca gacgtttacg cagcaagcgc tcgccaatgg gcgaacggca
    24181 aatgttgccg gtacaaacca ccaggattga gttaaatttt agttgggcca tgtttgaatc
    24241 caccgtgaag tctctgtcag gtcatgcacc ccggaaatcg tcggtaccag cagagaaatc
    24301 acacggttcc aacgagccag cggcgcggta gtgacataaa caatgtcgta aggttcaagc
    24361 tggaattccg ttccgagtat cattgccgat gcgtccttcg cattcagctg ataaatattg
    24421 gcgattttgc cattctgctt gttttgcgtt gcacgaataa caaaaatccc cgtcgcatca
    24481 gcgacatcct gattcatacc ctcagcgtta cccagcgctt ccgccagggt cataccgctg
    24541 cggtccattt tcagcgtgct ctgtttaccc acttcaccca tcacgaagac tttcagatca
    24601 tcgttgcgcg gaataaataa aatatcgccc gggtgcagca gtttgttctg gcgcaagtcg
    24661 ccacgctgca tcagcgcata gaggttcact ttggtcttta cgccattctg ggtgagcacc
    24721 acgttgcgcc agtctgcatc tgcggtcaga ccgcccgcgg cgttaatcgc atccatgatg
    24781 gtcagaggaa tattggtaat cggctgctga cccgatttgc tgacttcgcc ggtgacgtac
    24841 gctttttgcg atcggaacgc cgcgacgctg acatcgactt gcgggctttc aatgactgaa
    24901 tcgagacgcg ctgtaatctc gttgcgtacc tgggtaagtg ttttcccggc cactttcagg
    24961 cgaccaatat aggggtagaa aatcgcacca tcggcgttaa cccagttgcc ggtatcactg
    25021 gcgctacggt attgccctgc gggggtggtt agttccgggt gatcccagac cgtaaccatc
    25081 aacacatcac caatcccgat acgatattca tagtttgcga tttgctgctc cagctccggg
    25141 ttagcctgcg acataatcgg tttggcgcgc aactgatcaa tcaatcctgg cgtgacggga
    25201 tagatattga ccattttgtc caggtcatac tgattgtcag gtagttcaat gacatccttg
    25261 ttactggtcg ataaattctg accaggaaca agggtacatg ctgtcaaaga cagaacagcc
    25321 aatgtcagta ccgaaaactt aaaaatgttc tttttcattg gtttatattt atcagtcaat
    25381 gtaatgttat gaattacgtc aggttattgt ccaaccggta cttctcgctc tgccgtcatt
    25441 ggatagagct ggtatttgcg tgacagcatt tgtccaccat cacgcgtcaa tggtgtccac
    25501 aaaaccaccg cgcggttgcg gttaggtcca atggtcatca agtcgaatgg aatcgagata
    25561 taaaaacctt tactaaagcc gccttcgccg tagtcatctt tagatacgtt actgattgcc
    25621 gcccataccc ctaccgccac gccgctgtca aagcgtttgg cgacgtcgat cgttgcgcct
    25681 ttatcttttg ccagatattg cccaacgcta agtttcatca gtacgccatt gagcgtcggc
    25741 gggttccagt aagccgtcac gaaacctgtt ggcgtggaat aatcggtgaa acgcatcatg
    25801 ttgtcccagt cacgctgctt cacgtagtta acgtccacac ccagcgccca gctggcatct
    25861 agcgggcgat aaagcagctc ggaaccgaca ccggcataca tcgtttccag ataaccgcca
    25921 tacacctggc catagaaacc attgcctaag tcagcaaaat agttcgcctg caagttgttg
    25981 agataaacgt cattgcgaac gtagtcacgg atatgcgtgc gcacgcgggg cagggtagag
    26041 tccgcgggca acagcgaaga cttaaacttg tcgtagttgt tgtaaatatt ggtgaaaata
    26101 ccgccatcaa gcagcaggtg gtcggtaaac cagtagcggg cactggacat cagccccagc
    26161 tggaacatat agaaatcttc cgggccgccc agcgactgtg aaagtgttgg gttgaaagag
    26221 tagctaaagc gatcttcacg aatacggtag ccccgaccaa aggcagaaag atcttctgct
    26281 tcaacacgtt gttgttgcag ttgctctgat tgaccaggcg ctgtaccagc cagctgtttg
    26341 cgcaggctgg ctacgtcggt ttcggtagtc accatcgcca tatgctcgcg cttttgcgtc
    26401 acgctaatct tctcaacgcc ttgcggcagg ttattcacca gaatccggtt ggcacgatcg
    26461 accgcttcac gagagtcacg gtatttgtat tgctgaccag acatatacag cgtcttatcg
    26521 cgtagctgaa tttctggcgc gtcaaagccc gcgttatact tcagggcggt aagttggttt
    26581 gctaccgtgg tgtactgcaa tccttcagat tcaggcgcag gttgatatgc cggttttggc
    26641 gtatcgcgca gggcagggcg cagatcgttg aaattggtcc gtaaagtgaa accaaacatc
    26701 aacgtgttac cgcgttcata actcaggttg agatctgccc agctggcagc gcgataaact
    26761 gcaccgacgt tgaaatggct tgcctgaggc agtttgccag cgaaatcatt ctggtaattg
    26821 ttgccgtcgt attcgagttt cagacgcagg ggattccacg gcgtttgata ctcaatgccg
    26881 ccaaagatgg aagccgggcc acgaaagata tcgctaaagc tgatatcgcc cgcatcgtga
    26941 gactctgcgc gatgacaata tttatcgctc acccggcaaa acgggttggt aatattgccc
    27001 gcattaccgg cgtagcccca tgccatcccg agggtgaaat caaatggccc cgcttgcttg
    27061 ctggccacca gatactcacc gtcaaacaga cccgtaccag caatatcacg tttaccaaac
    27121 gccacttgcg gtagccagtg accttcttcc cacaggcgaa gtttaaaatc gaatgatttg
    27181 tctttgtatg actgatcgcc gctgaaatct tcccactggc tatatttgcg tgtgcgcaca
    27241 tccgtataac gaatggtgcc ttccagccat gggaacagcg ccacggaggt ggagtagaac
    27301 cggtattgat cgttatcccg gtagttgacg ctgaattcac cttccggtgc gatgcgcgca
    27361 tttggcatct gcaacaatcc tgtgccgcca aagtctgatt gcgacgaacc cagcggatcc
    27421 gggtaggtta atacttcagc atgacaggca ctggagacgg caatggccag gcagcttaaa
    27481 agataagaat tcttcttcat cagtcaggaa cacgttgcgt taaaacagac acaatctggt
    27541 catttaaatc agcatatttt tcgggcagta catgcgcaga aaagcccaac caaagctggc
    27601 tgccaggcgg tggttccaca tgtcttttat tccacagggc aacaggggcg acgacagtct
    27661 caccctcagg cgtaatcacc ataacgttat tcttgtccgc gcctgcaagg cgggggtgat
    27721 cttgcagata atcggtaacg ctacggcctg cctgccaggg caattgcccg gccccagaaa
    27781 ccgcacccag cagagtaata gtcaccggac gctgtacggt atagagcgta tagtccccga
    27841 ccaacggggg attgctgttc tcatccactc gtacaaaatc agggtccagt ttgaccggta
    27901 agcgaccagt gatattgaga ttgagtaact gctggcgcac ggatttaatc gttgccgcca
    27961 catcgtcgtc agcttctgct tcccaggaag caagctgtgc catgacatgt tggtagtctt
    28021 taagcgcttt agctttcgcc gcagagtcgg tcagcaaggc accaggccac cagaggcgat
    28081 cgcgcagttg cggttgtgtc accaactgga caacattttc cacggggcca acggaaagtg
    28141 tctgttgttc gccaggcaga taaatagtca ccgttccttg cgcaaaggca tggggtgtca
    28201 ttacgtaaag tacgctggca atgaaatacg actgtaattt attcatggtg cgggtttaag
    28261 aaacgtcatt tctacgggaa taacccctgc gcctaacatt tgccgacttt gacgaacctg
    28321 gccagtagcc gaatcgaccc agaatgtgtt ttgccactgt ttttctggcc gcgtggaagt
    28381 cacctcttcc tgccagacat tacacagcac attgcgaccc gcaatattca gcgtctctaa
    28441 tccagcaaag gaaaaagtag aactcagggt cgcagagcga aaatggttgt cttcactcca
    28501 gagaatatcg cgcgtccagc ggctgccatc ttttattgcc agcgcgttac gcagagggtc
    28561 ctgcccggaa tttgtcactt ccagcagatt attattaagt ttgacggttt ttaaaagacg
    28621 tccattgtgg gtaaccagca tggcgttatc ctgggataac catttgcttt gttcttgttc
    28681 tatatatccc agtaccacaa agattcgttg cccttcatta aggcgtaaat acatcgtgga
    28741 ataaggcaac gcctggatct gctgatccgc tacggtgata tcctgattat cgaaaaggct
    28801 ggcgcgaaat gtatcgacca tactttgctg gctgtgcgta catcccgcaa gaaatagtgc
    28861 gaaaatcgat agaataagag ggcgcacctt taatccttga aaggttaatt aaaaaaatgc
    28921 aaatctctaa agcccggtac gaatatcgca accgggtatc atttaacttt aatttcgacg
    28981 cgttaaagca gtattagcgt gtagtggacg tggtggttga ggtgttatga gaaccgccat
    29041 cgccactgtc atttgctgca acaactgcca acgtcgccac accaacagcc gttgccacac
    29101 cgacaaccgt cgccgtgccg acagcgccag cagccacgcc accagtggta gccgcagtcg
    29161 tggttgcacc ggtagtggtc gttgtggtgc cagtagtacc cgtcgttgcc tcggtcccgt
    29221 ttgctgcttc aggggcagca aatgcagctg gcgtcgtcaa catgaaggcc ataaggattg
    29281 cagaaagctt gtgtttcata acttttcctt tattcatcgc atggacaata cgtacgcttg
    29341 agatcaggat tgtctacttt gagtataaca tccttgtgaa atctcccttt cctcataccg
    29401 cttgcactat atactgcggg taggaaaggc gcgcagaggg aaatataaga ttgttaacta
    29461 tccccctctg gaaatacgac ttgtgagcat a
//
LOCUS       DAFNBJ010000052        28809 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000052 DAFNBJ010000000
VERSION     DAFNBJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.052"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..517)
                     /gene="rcdA"
                     /locus_tag="L2X49_002112"
     CDS             complement(<1..517)
                     /gene="rcdA"
                     /locus_tag="L2X49_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBN4797194.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRM"
     gene            601..1809
                     /locus_tag="L2X49_002113"
     CDS             601..1809
                     /locus_tag="L2X49_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4797195.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHISLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            1809..2624
                     /gene="ybjI"
                     /locus_tag="L2X49_002114"
     CDS             1809..2624
                     /gene="ybjI"
                     /locus_tag="L2X49_002114"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBN4797196.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            2710..2994
                     /locus_tag="L2X49_002115"
     CDS             2710..2994
                     /locus_tag="L2X49_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797197.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(3035..4267)
                     /gene="mdfA"
                     /locus_tag="L2X49_002116"
     CDS             complement(3035..4267)
                     /gene="mdfA"
                     /locus_tag="L2X49_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBN4797198.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            4552..5148
                     /gene="ybjG"
                     /locus_tag="L2X49_002117"
     CDS             4552..5148
                     /gene="ybjG"
                     /locus_tag="L2X49_002117"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBN4797199.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGFTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5206..5964
                     /gene="deoR"
                     /locus_tag="L2X49_002118"
     CDS             5206..5964
                     /gene="deoR"
                     /locus_tag="L2X49_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBN4797200.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(6011..7213)
                     /gene="dacC"
                     /locus_tag="L2X49_002119"
     CDS             complement(6011..7213)
                     /gene="dacC"
                     /locus_tag="L2X49_002119"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBN4797201.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDTWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            7460..8086
                     /gene="gstB"
                     /locus_tag="L2X49_002120"
     CDS             7460..8086
                     /gene="gstB"
                     /locus_tag="L2X49_002120"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HBN4797202.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(8083..9198)
                     /gene="yliI"
                     /locus_tag="L2X49_002121"
     CDS             complement(8083..9198)
                     /gene="yliI"
                     /locus_tag="L2X49_002121"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HBN4797203.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(9309..9692)
                     /gene="bssR"
                     /locus_tag="L2X49_002122"
     CDS             complement(9309..9692)
                     /gene="bssR"
                     /locus_tag="L2X49_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBN4797204.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            9905..11230
                     /gene="rimO"
                     /locus_tag="L2X49_002123"
     CDS             9905..11230
                     /gene="rimO"
                     /locus_tag="L2X49_002123"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBN4797205.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(11277..12605)
                     /locus_tag="L2X49_002124"
     CDS             complement(11277..12605)
                     /locus_tag="L2X49_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBN4797206.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(12613..14961)
                     /locus_tag="L2X49_002125"
     CDS             complement(12613..14961)
                     /locus_tag="L2X49_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN4797207.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(15139..16050)
                     /gene="gsiD"
                     /locus_tag="L2X49_002126"
     CDS             complement(15139..16050)
                     /gene="gsiD"
                     /locus_tag="L2X49_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBN4797208.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(16053..16973)
                     /gene="gsiC"
                     /locus_tag="L2X49_002127"
     CDS             complement(16053..16973)
                     /gene="gsiC"
                     /locus_tag="L2X49_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBN4797209.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(16991..18529)
                     /gene="gsiB"
                     /locus_tag="L2X49_002128"
     CDS             complement(16991..18529)
                     /gene="gsiB"
                     /locus_tag="L2X49_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBN4797210.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKVQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(18549..20420)
                     /gene="gsiA"
                     /locus_tag="L2X49_002129"
     CDS             complement(18549..20420)
                     /gene="gsiA"
                     /locus_tag="L2X49_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBN4797211.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(20407..21372)
                     /gene="iaaA"
                     /locus_tag="L2X49_002130"
     CDS             complement(20407..21372)
                     /gene="iaaA"
                     /locus_tag="L2X49_002130"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBN4797212.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            21576..22811
                     /gene="moeA"
                     /locus_tag="L2X49_002131"
     CDS             21576..22811
                     /gene="moeA"
                     /locus_tag="L2X49_002131"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBN4797213.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            22811..23560
                     /gene="moeB"
                     /locus_tag="L2X49_002132"
     CDS             22811..23560
                     /gene="moeB"
                     /locus_tag="L2X49_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBN4797214.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(23636..24298)
                     /gene="fsa"
                     /locus_tag="L2X49_002133"
     CDS             complement(23636..24298)
                     /gene="fsa"
                     /locus_tag="L2X49_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBN4797215.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            24429..25328
                     /locus_tag="L2X49_002134"
     CDS             24429..25328
                     /locus_tag="L2X49_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBN4797216.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            25334..27766
                     /locus_tag="L2X49_002135"
     CDS             25334..27766
                     /locus_tag="L2X49_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBN4797217.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALACEMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            27912..28727
                     /gene="ybiV"
                     /locus_tag="L2X49_002136"
     CDS             27912..28727
                     /gene="ybiV"
                     /locus_tag="L2X49_002136"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBN4797218.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
BASE COUNT         7180 a         7620 c         7008 g         7001 t
ORIGIN      
        1 ccatcctcaa aatctcctcg cgcgatagcg gcttacggtc ggtgacaaaa tgcagcgtca
       61 tcccctcaat aaacgcatca agcgcgcggg cggttccggg ttcaaaccat tgttcgagcg
      121 tttgctgact gcgctgcatc cagttttgca ttaccgtttt taatagcggt tttcggctag
      181 ccagcgcgta gagctggtac atcagctcca tgttatccgg cgttgcaacc tgtgagctgt
      241 agatcatatc ggtgatagcc tggcatgcgc cttgagcatc actaacatcg ctaaaaaatg
      301 cctgatattg ccgggacata atctcagtaa aactgctgaa cgcctccagc aacaactcat
      361 caattcctga aaaatagtag gtcatcgatc ccaacggtac cccggcaagg gtagcaattt
      421 tgcggtgcgt aacagcatgt attccgtaaa gtttcaccgc ctccagcgtg gcctggataa
      481 ttttttctcg ccgttgcgga tcgttagcgc gacgcatgtc atttcctctg tctgcaattg
      541 tgtacaaatg tacacaacct tgctaatgtt gtcttccctc ttcttatttt gacgtgatct
      601 atgaccgtaa attcttcacg taatgcattg aaacgccgaa cttgggcgct gtttatgttc
      661 ttctttttgc caggcctgtt aatggcgtcc tgggcaaccc gtacgcctgc tatccgcgat
      721 attctctctg tctcgatcgc tgaaatgggc ggtgttctct ttggtctgtc gatcggttcg
      781 atgagcggta ttctctgctc ggcgtggtta gtgaaacgct ttgggacacg taatgtcatc
      841 ctggtcacga tgtcctgcgc attgatcggg atgatgatat taagtctggc actctggctg
      901 acatcgcccc tgctctttgc cgttggtctc ggcgtctttg gggcaagttt tggttctgcg
      961 gaagtggcga taaacgttga aggtgccgcc gttgagcgag aaatgaataa aacggttttg
     1021 ccgatgatgc acggttttta tagcctgggc acgctggcag gcgctggtgt cgggatggca
     1081 ctgacggcct ttggcgttcc ggcaacggtg cacatttcat tggcggcgct ggtaggtatc
     1141 gcgcctattt atatcgccat tcaggcaatc cctgacggta cgggcaaaaa tgctgccgat
     1201 ggcacccagc atggcgaaaa aggcattcct ttttatcgcg atatccagtt gctgttgatt
     1261 ggtgttgtag tgctggcgat ggcctttgcc gaaggttctg ccaacgactg gttaccctta
     1321 ttaatggttg acggtcacgg ttttagccct acttccggct cgctgattta tgccggtttt
     1381 accctgggga tgaccgttgg acgcttcacc ggtggttggt tcatcgaccg ttacagtcgc
     1441 gttgccgtgg ttcgggccag tgcactaatg ggggcgttgg gtattgggct gattattttt
     1501 gtcgatagcg cctgggtcgc aggagtgtct gttgtacttt ggggactggg tgcctcgctg
     1561 ggcttcccgc tgaccatttc tgccgccagc gataccggcc ccgatgcacc aacccgcgtc
     1621 agtgtggtag caacgaccgg ttatctggct ttcctcgtcg ggccgccgct gctgggctat
     1681 ctcggcgaac attatggatt acgtagtgca atgctggttg tactggcgct ggttattctc
     1741 gcggctattg tcgcgaaagc cgtcgccaaa cccgatacca aaacgcagac ggcgatggag
     1801 aatagttgat gagcattaaa ttaattgcgg tagacatgga tggtactttc ttaagcgatc
     1861 aaaaaaccta taaccgtgag cggtttatgg ctcagtatca gcaaatgaaa gcacaaggca
     1921 ttcgctttgt ggtcgccagc gggaatcaat attatcagtt gatctctttc ttccctgaaa
     1981 ttgcgaatga aattgccttt gtggctgaaa acggcggctg ggtagtgagt gaaggcaaag
     2041 atgtttttaa tggcgagctg tcgaaggatg cctttactac tgtcgtggaa catttgctga
     2101 cgcgcccgga agtggaaatt attgcctgcg gaaaaaatag cgcctatacg ctcaaaaaat
     2161 atgacgatgc catgaaaacg gtggcggaaa tgtattatca ccgtctggaa tacgtcgata
     2221 actttgacaa cttagaagat attttcttta agtttggcct gaatctttcc gatgaactga
     2281 ttccacaagt acaaaaagca ttacatgagg ccatcggcga tattatggtg ccagtccaca
     2341 ccggcaacgg cagcatcgat ctgattatcc ccggcgtaca taaagccaat ggccttcgcc
     2401 aactgcagaa attatgggga atagacgaca gcgaagtggt ggtctttggc gatggcggta
     2461 acgatattga gatgctgcgt caggcaggct ttagttttgc aatggaaaat gccggcagcg
     2521 cggtcgtcgc agcggcaaaa taccgggcag gctccaataa ccgtgaaggc gtactggatg
     2581 tgatcgataa agtgcttaaa cacgaagcgc catttgacca ataaaaacgg cagcacgccc
     2641 ggcaacacaa tacctgaatg aaggctgcat aattcgcgga aattaacccg ctgcattatt
     2701 tcaggcgaaa tgattatgaa aaattgtcta ctgttgggcg cgcttttaat gggctttact
     2761 ggtgtggcga tggcgcaaag tgtcaccgtc gatgtgccga gtggatacaa agtggttgta
     2821 gttcctgatt cagtcagcgt tccacaagcg gtcagcgtcg cgactgtgcc gcaaaccgtc
     2881 tatgtggctc ctgcgccagc tccggcttac cgcccgcatc cttatgctcg ccatcttgcc
     2941 agcgtaggtg aagggatggt tatcgaacac cagattgacg accatcacca ttaattcgag
     3001 aatgcctgat cgcacaaagc agtcaggcat ttttttaccc ttcgtgagaa tttcccatct
     3061 gtttatcttt taaaaagata accatcagcg acagccacaa aattccgttg acaaggttga
     3121 agagattaaa ctgtccgttg cccccgttca gccaggcatg tttgctgatt tcaataccaa
     3181 cggtaaagat cagcatttgc agcattccca tcgccgcaga aaccgtacct ttactcatat
     3241 cgctggcaaa cagggttaat cgcaccagtc ccgcattcgc cagaccaata ccgaaagcat
     3301 aaatacttaa cccggcagtc atccataaat acgcgtgcga tgagataacc gttgccgcag
     3361 cagcgaccaa cagaccaatc ataatcggcc agccgcccat aattatcagc gaacgtacgg
     3421 tgcggcgcga ggtcaaacgc gctaacaaca agttacccgc aattaacgcc ccgaaaatag
     3481 gcacttgcag caagccatat tcatagctgc tcaactgctc gccggtaatg atgataatcg
     3541 gcgactgggc gatccacgcc agcaatggca ggctaacgaa tcccagcgcc agcgcccccg
     3601 ccacaaagcg gccgttcttc agcaccagct tatagtcacg accgagttct ttcagtgaca
     3661 gtttctcgcc tatacgcgtg gcggtttcag gcatagctcg ttgcagaccg aaaaaggaga
     3721 tcgctgccaa tgcggcaaac aaaacaaaca tcccctccca gggcagcaca tggatccacg
     3781 ccgcgcccac cagcggacca agtagcggag caatcagcgc cacgttcgcc atcagcgcgg
     3841 tgatcttgat acaaaccgcc tcttcgaagg attcctgaat tgcggcgtat cccacagcgc
     3901 caatgaaaca gaggcttatg ccctgcaaga agcgcaacag ggtgaattgt tcaatgtttt
     3961 gcgccagcaa tattgccaga caggtgatga taaaccacac cacccccgcc agcatcaccg
     4021 gacggcgacc aatacgatcc gacagcggcc ccaggagcca ttgtaaaaac atcccgcccg
     4081 ccagatatgc ggtcattgaa gtaggaaccc aatcaatgcc cgcctgatat tgttccacca
     4141 cggccaacat accgggttga atcatatcgt tgccgatata ggttgaaaat tcgtaaagca
     4201 ccagacagag agggaaaagt aacgcctgac gtccaagcct ggcaccggaa gctaatttat
     4261 tttgcatgca atttcttcgc caataataat cgcgcagagt ttaataaaag cacagctaac
     4321 gagaaagcga attttgtagc tgaaaccacg gttaagcaca ttcttacatt attgcgagta
     4381 tagctacgct ttctttaagt tttatttaac ctatgcccgt tacaattacc caccgtaaac
     4441 aggccgcttg agggaaataa gacgatgccg ctttacccag tttaacctgc actttattct
     4501 caacgacttg cctgtattgg ctccctttta atcactttgc gtcgggaagt tatgctggaa
     4561 aatttgaatc tctctctatt ctctcttatt aacgcgacgc cagactcggc tccgtggatg
     4621 atctcgttgg cgatttttat tgctaaagat ttgattaccg tggtaccgct gctggccgcg
     4681 gtgctttggt tgtgggggtt tacagcacaa cggcaactgg tgataaaaat cgctatcgcg
     4741 ctggcggtca gcctgtttgt gtcctggacg atgggacatc tttttccgca cgaccgaccc
     4801 tttgtcgaaa atatcggcta taacttcctg catcatgcgg cggatgactc attcccaagc
     4861 gatcacggta cggtgatttt cacctttgca ctggcatttt tatgctggca tcgcctgtgg
     4921 tccggctcac ttttaatggt gctggccgtc gtcattgcct ggtcgcgcgt ttatcttggc
     4981 gtccactggc cgctggatat gctcggtgga ttgctggcag gtatgattgg ctgccttagt
     5041 gcccagatta tctggcaagc gatggggcat aaactctatc aacgtctgca atcgtggtat
     5101 cgcttctgtt ttgcattacc gatccgcaaa ggctgggtgc gtgactgatc tctgctaaaa
     5161 agtgtagtat tgagcggctc gcttcaataa ctattcagag ggattatgga aacacgtcgc
     5221 gaagagcgta tcgggcagct gctgcaagaa ttaaaacgca gcgataagtt acatcttaaa
     5281 gacgccgccg ccctgcttgg ggtttcggag atgacgattc gtcgcgatct gaacaaccac
     5341 agtgcgcccg tcgttttgct cggcggctat attgttctgg aaccgcgcag tgccagccat
     5401 tacctgttaa gcgatcaaaa atcccgcctg gtggaagaaa aacgccgggc ggcaaaactg
     5461 gctgcgacac tggtagaacc cgatcagacc ctcttttttg actgtggcac caccacgccg
     5521 tggattattg aagcgattga taatgaaatc ccttttaccg ccgtttgtta ttcgctaaat
     5581 acctttctgg cgctgaaaga gaaaccccat tgccgcgcat ttctgtgcgg tggtgaattt
     5641 cacgccagca acgccatttt caaacccatc gattttcagc aaacgctgaa taatttttgc
     5701 ccggatatcg ctttttattc tgcggcgggc gtgcatgtca gtaaaggcgc tacctgtttt
     5761 aatcttgaag agttgccggt aaaacactgg gccatgtcga tggcgcaaaa gcatgtgctg
     5821 gttgtcgacc acagtaaatt tggcaaggtg cgtccggcgc gcatgggtga cctgaaacgc
     5881 tttgatattg tggtgagtga ttgttgcccg gaagatgagt atgtgaagta cgcgcagacg
     5941 cagcgcatta agttgatgta ttaatgacgt ataaccggat gacgtttcgc gccatccggt
     6001 tatcagaaga ttaagagaac cagctgccga accactgatg gaatttcatc atcacgaaat
     6061 cccacatccg accaaagaat ccgccctctt ccacattttc catcacgatc agcggacgct
     6121 gctcaatgga tttaccgtta agctggaaat caatggtccc gacaacctga ccttttttca
     6181 gcggtgcggt aagctgcggt tccgttaacg tgtagctcgc cttcaggttt ttcagctgcc
     6241 cacgcggtat ggtcactgag cccgcttcgc ctgccccaag attcacttcg ctcttatcac
     6301 caaaccagac gcgctgagtc acaaaggtgg catcaggttt aattggcgtc acggtttcaa
     6361 agaagcggaa accccaggtc aataatttct cagactcatt aaaacggata cggtcagttt
     6421 tcgcccccag cactacggag attaaacgca tatcgccctg ggtagccgaa gcaaccagat
     6481 tatatcccgc gcctgccgtg gttcctgtct tcatgccatc aacattcaga ttgctgctcc
     6541 acagcagacg gttacggtta ggctgacgaa ttttgttgaa ggtgaactct ttctctttat
     6601 gaatggcgta ctcttccggc acatcgtgga tcaatgcttt acccagcaat gccatatcgc
     6661 gcgcggtact gaactgcccc ggcgcatcca gaccgtgcac cgtctggaag gtagtgttgg
     6721 tcagacccag ttttttggca taaccattca tcaaaccaat gaatgactcc tggctcccgg
     6781 caacgtaatc ggccagcgca atacaggcgt cattaccgga ctggataatc acacctttgt
     6841 tcaagtctgc caccgaaacc tgatcgcccg gtttgaggaa cattaccgat gaaccacgca
     6901 gtgccggatt tcccgtcgcc caggtatctt taccgaccgt caccatatcg gtgagtttaa
     6961 tcttatcggc cttaagcgcc tgcccaacca catagctggt catgatttta gtcaggctcg
     7021 cgggatccag tttctcatcc gcgttgcctt ctgccagcac tttaccgctg gcgtaatcca
     7081 ttaaaatcca tgcacgcgca tccacgctcg gcgcttcaac ggtttgttcc gccgcgaatg
     7141 ccgttggggc aaaaaggaat aaaaatgcag aacccgctgc aagaccacga aggagagagg
     7201 agtattgcgt cataaatgcc acccgagtat ccattcaaaa aaaatacgtt atatcgccgt
     7261 atgataagtt cggtgattaa taacgcacac acaaacttaa agaaaccgta agttggtaaa
     7321 gtttttaaag tttatgcaat atcctgccgc ttcgctgctt ctggtgtcat ttttttgcca
     7381 gctgttgctt aaaagttaac tttacttcac catcggagtc ctgttcagcc tgtggctgcc
     7441 tgtcggaaaa ggggtgagta tgattacgct gtggggtcgg aataattcaa ccaacgtaaa
     7501 aaaagtattg ctgacgctcg aagaactgga actaccttat gagcaaattc tcgcgggccg
     7561 tgagtttggg ataaatcacg atgctgattt tctggcgatg aaccctaacg ggctggtgcc
     7621 gttgttgcgt gacgacgaaa gtgatctcat tctttgggaa tcaaacgcca ttgtccgcta
     7681 tctggcggca cagtacgggc aaaaacgtct gtggatcgac tcaccggcac gtcgtgcgga
     7741 agcggaaaaa tggatggact gggcaaacca gacgctcagt aatgctcatc gcgggatcct
     7801 gatgggatta gtcagaacac caccggaaga gcgcgatcag gccgccattg atgccagttg
     7861 caaagagtgc gacgccctgt ttgccctgct cgatgcggaa ctggcaaaag taaaatggtt
     7921 ctctggcgac gagtttggtg tgggcgatat cgctatcgca ccgttcatct acaatttgtt
     7981 taacgttggc ctgacctgga caccgcgtcc aaatctgcaa cgctggtatc agcaactcac
     8041 tgaacgcccc gcagtgcgca aagtggtgat gattcccgtt agctaattgc gtgggctaac
     8101 tttaagtaat tccccactgg actcgtcggt gagaacgtat aaataaccgt cgggtccagt
     8161 gcgaacatca cgaattcgct gccctctgtc cgttaaaata cggccatctt ctgtcacttt
     8221 gtcgccgttg acgctcatca caatgacatc tttatctttc agcgcgccaa taaataattt
     8281 ttgctgccac tgggggaatt tatcgctgtt atagaaggcc atgccgctca cagcgggcga
     8341 atctttccag taaaaaacag gttgctcggt cccggcgacg atctcccctt tcgcttccgg
     8401 tatcttaaag cctgaatagt tgattcccca ggttgccagc ggccagccgt agtttttgcc
     8461 tttttgcggg atattaattt catcaccacc gcgcgggcca tgttcattca gccacagtgc
     8521 attactccac ggattcatcg ccattccttg cggattacga atgccataag accagatctc
     8581 ggcgcgcaca ccggattcct ttataaaagg attatcatcc gggatttcgc cctggtcggt
     8641 cagacgcacc agtttgccct gtaatttatc cagatcctgc gccgtcgggc gctgattgtt
     8701 ttcgcccaga gcaataaaaa gataaccttt accgtcgaat accagccgcc cgccaaaatg
     8761 gttgccggta gacagttttg gcatctggcg gaagacggtg cggaagtcgg tcacttttga
     8821 gagatcatca cttaagcggc cataacccac agcagttccg gctttgccat catcgccaac
     8881 ttcggaataa cttaaccaga tgcggcgaga ctgagcaaaa tcaggcgcta aaaccacgtc
     8941 cagcaggccg ccctgcccgt gcgcccaaac gtccggaact ccggaaagcg gcgcagataa
     9001 tccttttcct gcttgccagt gacgcaactc gccgccgcgc agagtgatta acataccgtg
     9061 attatcgggt aaaaaggcca gtgcccaggg atggtcgagt ttgtcttgca gtacttcgac
     9121 atttaccgtt gcaggagccg cccagagagc ggaagaaaga caaataaggg gcacaaggaa
     9181 aaaggattgt cgatgcataa cgcctccgga ttgactctgg cttaaagcgt agtcagtgga
     9241 ggagataaca aattcatttt tacaaaaact taaacatgaa gggggagacg ctttctcccc
     9301 cttagttttc aggccttctc aagcatggcg tgcttctgca ggctctggat actcagcgtt
     9361 aagctcatca gacaattttc aagcttatcg gcgttgacgg taataacagt cgggcaatca
     9421 tggtgcccac tcatcaaaca tactgcggct gtcgctaatg cttcttcagc atgatgaaga
     9481 gcactccact cttcctgatc cagatgaaga ttcaaccgca gcgatttatc gtgcagttcg
     9541 cgattcagtt taaaaaagtt atctcgtaga tgattgcttt cgctgacgga catgtatcct
     9601 tttgccttcc tcagttgcac gtatgcagcg aggtcaacgc gtgcgtcgat caaccggttc
     9661 agcagatcga ttcgctgtct gtcaacgaac atacttcgat cctcctcttt ccagccttgt
     9721 tgcaattaaa tagtatggct attttttgag caaaatgctg tccaggatca attcttcacg
     9781 ccgaattaac aaaatttaca tttccttcgc ctccgccagc gaatgagatt gcgatacaaa
     9841 ttttatccag aactctgtag aatccctgcc ctgacctttc ctgtaactga atatttttta
     9901 agctatgagc aaagtaactc cccagccgaa aatcggcttt gtttcccttg gctgtccgaa
     9961 aaaccttgtc gattcagagc gtattctcac cgaactccgc actgaaggtt atgacgtggt
    10021 accgagctat gacgatgcgg acatggtgat cgtcaacacc tgcggcttta ttgacagcgc
    10081 ggtacaagaa tcactggaag ccattggtga agcgttgaat gaaaacggca aggtaattgt
    10141 gaccggttgt ctgggggcaa aagaagatca gatccgcgaa gtccacccga aagtgctgga
    10201 aattaccggg cctcatagct atgagcaggt tctggagcac gttcatcact acgtgccaaa
    10261 accgaaacac aacccattcc tgagcctggt gccagaacaa ggtgtgaaac tgacgccgcg
    10321 tcattatgcc tatctgaaaa tttcggaagg ctgtaatcac cgctgcacct tctgcattat
    10381 tccgtctatg cgcggcgacc tggtgagccg tccgattggc gaagtgttaa gtgaagcgaa
    10441 acgtctggta gatgcgggcg ttaaagagat tctggtgatc tcgcaggata cttccgccta
    10501 tggcgttgat gttaaacatc gtactggctt ccacaacggc gagccggtaa aaaccagcat
    10561 ggtcagcctg tgcgaacagt tatcgaaact ggggatctgg acacgtctgc actacgttta
    10621 cccttatccg catgtggacg acgtcatccc actgatggca gaaggcaaaa tcctgccgta
    10681 tctggacatt ccgttgcagc acgccagccc gcgcattctc aaactgatga agcgtccggg
    10741 ttctgtagat cgccaactgg cgcgcatcaa acagtggcgc gaaatctgcc cggaactgac
    10801 cctacgctca acctttattg tcggcttccc tggcgagaca gaagaagatt tccagatgct
    10861 actcgacttc ctgaaagaag cgcgtctgga tcgcgttggc tgctttaaat acagcccggt
    10921 tgaaggtgca gacgccaatg ccctgcctga ccaggttccg gaagaagtta aagaagaacg
    10981 ctggaaccgt ttcatgcagc tgcagcagca gatttccgcc gagcgcctgc aagagaaagt
    11041 gggccgtgaa attctggtga ttatcgacga agtggacgaa gaaggcgcga ttggtcgcag
    11101 catggcagat gcaccggaaa tcgacggcgc ggtttatctc aatggtgaaa ccaacgttaa
    11161 gccgggtgat atcctgcgtg tgaaagtcga gcacgccgat gagtacgatt tgtggggtag
    11221 ccgggtttaa ttaattcagg ccgggcaaaa tcatttgtcc ggcctgcaaa taatgattac
    11281 tgccagctaa tctgagccct gccgtttttc ttactcttat acagccgctc atccgccagc
    11341 attttcaagc gttcgacggt ggtacattca tttgaatgcg ctacaccgat actggtactt
    11401 aaattaatgc ttcccacctg gaaatgaacg atttctttta gaatcgatag caactcttcc
    11461 agcgttccac ccgtataaat caccgcaaat tcgtcaccgc cgaagcggta gattttacct
    11521 ttatctaccg ggatctgtga tttaaggatt ttcaccactt tcatcaacac ttcatcgccc
    11581 agcaaatgac cgaaggtgtc gttaatgttt ttgaatttat cgacgtcaaa caggcacaaa
    11641 taaccgcctt tttccggcag cgcttttaaa tcttcgtcaa aggattttct gcccattgct
    11701 ttggtcaggt cgtccaggaa cgccgagtta tacatactgt agagtttttt gacatcggca
    11761 gccgaaaaga aaagcccgcc atccagtacg cgttcaaaat ggttattaat gatggcttct
    11821 ttctccacaa taccacgatt aatcactctg cgtaccagat agaggcaact ggtacccgat
    11881 atcactgcca ccaatgcggc aaagaaaata atcaatgtac agacccgcaa ataaacatct
    11941 tttttatacg caaatacgta caatccggtc ggcaaatagg cgaagtgctc catcgcttcc
    12001 gaaacggtga agatggcgtt tttagcataa tattcatcac cggtcaccgg tttaccgttc
    12061 ttaaatagag agaacatcag gttctcgctg tccatcgcca gcgattttga cagtaaagaa
    12121 aaacgctcca gaccaagcaa aaatatcaga ctacgattgt gagttaccgg atagaggtaa
    12181 tagacgaagg ttttaccgtc aaaagagata aaccctgtat ttttgacatg tcgatacttc
    12241 acaacctgca taatatctgc acaataattt ttctcgaact cctttacctg aatacattcg
    12301 tcactggaaa tcagataagg accgacatcg tacaaataag agggggaaac cgagtcggcg
    12361 taagctgtgc gcaaaccaac caacagctgt tcctccgtgg gatttagcgt gttgataaga
    12421 tcaattgcca cgctacgccg atattcagca atatttgcca ccgcaaaagc agtatcaata
    12481 acggactgtt tcaggctata cacccgttcc tttaccattt gagtgtagat cccgcaggca
    12541 actgctgaaa tcatgataaa aatcagcaga ctgactgtaa gtacgaactt attgattctg
    12601 gacatacgta aactactctt ttactaattt tccactttta tcccaggcgg agaatggcat
    12661 gggtttctgg tacagatacc cctgagcctg aacacatcct aatttaatta attcgcgcgc
    12721 aacgtcttcg ctctctacgc cttccgcaat caccgggatc cggttatagc gtgccagatc
    12781 gaaaataatg cgcacaattt ttcccgcagt accgaaaata tcgccggtaa attgcgagtc
    12841 cacctttatg ctgtctggat taatttcata aaaacgtttc aggtttgaca aaccggtgcc
    12901 aaagtcatca atcgatatcc gtaccccttg tttacgcagt tgttcaatat tgagataaaa
    12961 atgttcatta attcgctcga tcacggtttc agtgatctca acacagaggc tgttcgcctt
    13021 caggccattt tctttcatga tggtggtaaa acgctcatga aagtgcggtt cgtttaactg
    13081 ccagggcgaa acattaatat gcagtaagaa atcatccttc agcgacgcac gctgactaaa
    13141 atgtctgaat tcacccattg ccgttttaat aatctgataa cccagctcat tgataaaccc
    13201 gatatcttca gcaatggtaa tgaacttcag cggtgaaatg atcccccgct ccgcagaaac
    13261 ccaacggcac aacgcctccg cgcccagaat ggcgcgggtg ttaatatcaa cgatcggttg
    13321 atagacgagg aatagttcgc caatttccat tgcatggcga atattacgcg caatccagat
    13381 attctcttct atttcgttga gtttcgcgtc atcacaaaaa ataaactcgg agaacatatg
    13441 gtcacggata ctggaaatcg ccagtttcag tttttctgcg taactccagg cgttttcccc
    13501 ctgcaaaggt tcacaaatgg cagacttatg gatgcgcaga ttgcggtagc caaagctgct
    13561 gtcaaaatcg tttaccatcg ctattttacg ggtaaatgtc tgtacatttt cccgacacag
    13621 aagtaaatag agatcggcat tgaatgtcac gatatcgcag cattgcgcat atttctcggc
    13681 aattcgctga cacaatttcc tgacgatttt attgcgttca tcaacaccaa acaccgcttc
    13741 cacggcttcc agaccacaca gtttgatgat ccccacagcc agatactgat tattgttgga
    13801 ctcaataatc gccatatttt taataaaacc gctttcctga tcgtaagaga tcagtgagag
    13861 caatttattt gaccgttcaa tttcggcatc tttcatttcc gcgatcgaat ttttgatgct
    13921 gataatctct ttcgcatcgc cttcgcgaat tttcagagcc tcgatctgat ccgttgagtc
    13981 gggcaaaaac gccagcgtat taaccagctc ggtcatttca gaaaattgcc gacggaacac
    14041 acgagtggtc atgaaatata ccaccagcaa cacgataata atgaacgcaa acggtagata
    14101 aaaaagttta tctgatgcgt tcaataaatt accgccagga taataaaaat agatttcgaa
    14161 caatgcatta tttggcactg ctgatgtctt tgcaatatac tccgtatcga cagaatcaat
    14221 aatattataa ttaccttcgc tattataaat aatcagtttt tcacgagcga gttgattctt
    14281 ggcaatgatc tcatgacctt gtttcaaaaa tagaacttca tcattagcgc tgatttgatc
    14341 aagatactcg ttcagatacc gccagtttaa atcgacatga attttatcta ccgctaacaa
    14401 atcgtgatct ttgccgttga tacgtagcgt aaaagagata cattttttac ccggacgatc
    14461 aaagcaattg tatgccggat tccagctgtt gttagagtca tctgaagcca ccgaccacgg
    14521 tcgtttcgat gggttaaaat cggcaaaaat aacccgccct gactcgtcga caagcggtcc
    14581 atttacttgt tcctgatgat aaacaacgat tttatcattc gccctccgcg aaaagagata
    14641 atatttatta tcatcaagta ccagtcccac cgagtctaaa aatggcatca tttccatttt
    14701 cagaatcacg gcgcgttgaa tctcctctgt attttttaat aaatacagtg agtcctttaa
    14761 tgtcagggcc gaacgttcaa tatctttaaa gaaatagcca acgtttttct gaataagttc
    14821 aaagcggcta tcggcacgtt ttaccgcgcg ttccgatacc agttgatagt taatgatgaa
    14881 aaaaagacca ataaatgaca gtgctgccaa taaaaataaa attatcagcc ttatctttat
    14941 cttttcgtat aaactcagca tgttaataca tttaacaaaa taactatctg aacgctgtgc
    15001 cgctgcaaaa atagtccctt gctgcggctt tttaaccaaa ttacccgatt ctacaaaatc
    15061 agcagaaagt tacaacactt gcttagggca ataattgcct gtgaggtgat taccctttca
    15121 agcaatattc aaacgtaact atcctttaat tttcggatcc agcgcatcgc gtaaaccatc
    15181 gcccaacaaa ttgaacgcca gtacggtcag aaaaatagcc agggccggaa aaacagcgac
    15241 atgcggcgcg ataaccatat ccgctcgtgc ctcattgagc attgctcccc actctggtgt
    15301 cggcggctgc gcaccgaggc cgagaaatga caggctggcg gcagagataa tcgaggtgcc
    15361 aatgcgcatg gtgaaaaaca ccacgataga cgagacggtc ccaggcaaga tatgacgcaa
    15421 taaaatggtc atatcgctgg caccaatact gcgtgctgac tcaataaagg tttgctgttt
    15481 caacaccagc gtgttgccgc gcaccaggcg ggcaaacgcg gggatggaaa aaatggcgac
    15541 tgcgataatc acgttagcaa tgccgcttcc caacaccgca acaacagcga tcgccagtaa
    15601 aatacccggg aaggcaaaca gcacatcgca aatgcgcatg atcagccgat cccaccagcc
    15661 ttcataatat ccagccagca agcccagcaa cgtccctatc gccgcaccga taaacacggc
    15721 aaacacgccc gccgccagcg agatttgcgc accaaccagg acacggctga aaatgtcgcg
    15781 ccccagtgaa tcgacgccaa accagtgctg caaagaaggt ccgttattca gattgtcata
    15841 atcaaaataa ttttcggcgt catagggagc gatccagcgt gcaaaaatgg ccaccacaat
    15901 caataaaata acgaataatg cggcggtcat cgccatatgc tgacggcgaa atcgtcgcca
    15961 gaattcatgc cacggtgtac gcacctggtc aggtttgacc agtggcatgg cgtttaacac
    16021 cgcctgacgt cgccagttaa atagtcgcat ccttacttgt acctgatagc cgggttaatg
    16081 gcggcgtaaa gcacatccac cactaagttg ataagaataa attccagcga gaaaagcaga
    16141 atttccgcct gaatcaccgg gtaatcgcgc atttctacgg agtcaacgag taagcgtcca
    16201 agtccgggcc agttgaaaac tttctccacc acgatagaac caccgagcaa aaagccaaac
    16261 tgtaagccca tcatggtcac taccgggatc atcgcgttac gtagcccgtg tttgaggaca
    16321 acccaggttt cgctcacccc tttcgccctc gcggtacgca tataatcttc gcttaaaaca
    16381 tcgacaaacg acgcgcgggt aaagcgcgcc atcacggctg ccactgccgc gccgagcgtc
    16441 agggagggta aaatgtagtg ctgccaactg tctgctccca cggtaggcag ccagcccagt
    16501 tcaacggaga atacctgaat taaaagcatc cccagcgcaa atgccggaaa cgagatcccc
    16561 gacaccgcaa tggtcatact caatcgatcc ggccaacgat tacgccagac ggcggcgata
    16621 attcccgccg ccataccaaa tatcaccgcc cagaccatac tggttatggt cagccacagc
    16681 gttggcataa agcggctggc aatctcatcg gcaaccggac gacgcgacac catcgacagg
    16741 ccaaaatccc cctgcacagc attgctgata tagtgccaga actggtgata cagcggctga
    16801 tccaacccca gctgctgacg caccagttct ataacctgcg catcagcttc gggcccggca
    16861 atcaatcgcg ccggatcgcc gggcagcata tgcacaaata aaaacaccag caccgagacg
    16921 ataaacagcg tcggaatcaa ccccagtaag cgtttgataa cgtaattaag cattccactc
    16981 cctgcgttgc ttattgcaaa tccgcgtctt caaagctgaa gccggtgtct ggcatgatcc
    17041 aaaaaccggt caggttttta ctgtgtgccg acaccagttt ttctaccacc agcgggatcc
    17101 acggcgactc ttgccagatg atatcctgcg ccgccttata caagcgggtc ttttccgccg
    17161 gatcattagt tttcagtgcc tgagccagga agtcgtccac ctgtttattg ctgtaaaatg
    17221 cggtattaaa cagcgtcggt ggccagtttt gcgaggcgaa cagtggcgat agcgcccagt
    17281 ccgcttcgcc ggttgaagcc gaccagccag tgtagaacat ccgcacgccg ctctcttttt
    17341 gccctttacc ttcaacttct gccgcccgct gtccggcatc catcgcagtc acctggactt
    17401 taatcccgac ctgcgctaac tgctgctggg taaattgcag cactttctgc gcggtgctgt
    17461 ggttatgtga cgaccacagc gtggtactga aaccgttggg atatcccgcc tctttcagta
    17521 attcgcgcgc tttcactgga tcgtaaggcc acggtttata actttgcgcg taggcgatac
    17581 ttggcggtac cacaccagta gctggcgttg catagcccgc aaaggcaact ttcaccagcg
    17641 ccggacggtt aatggcgtaa ttcagcgcct cacggacctt cgggttatcg aacggctttt
    17701 gcgtcacgtt catactgata taacgctgca taattgacgg actggccatc aactcgatat
    17761 ttttgttttt ctccagcagt gtggcctgct cgtagggaat ggggaaagca aactgcgctt
    17821 caccggtttg cagcattgcc gcgcgggtgt tgttatccgc caccggacgc caggttatgc
    17881 tgtccagttt gggcaatcct ggctgccagt aacccgcgaa ttttttcacc ttcacaaaat
    17941 cggtctgatt ccaggtatcc agttcatacg gtccggttcc caccggatga aaaccaatct
    18001 ccttgccata tttttccagc gctgccggtg aaatcatcgc ggtcgccgga tgggcaagaa
    18061 tattaataaa cgctgagaac ggctgtttga gggtaatctt tactgttgtt ggatcgatcg
    18121 cctccgtttt ggcaatattc ttatacaggt tatagcgttt aagatgattc gccggatcgc
    18181 tggcccggtc cagattcgct ttcaccgccg cggcgttgaa atcggtgcca tcctggaatt
    18241 taatgccttc ccgcaatttc acggtgtaag taatgccgtc atcggaaacg gtataactct
    18301 ccgccagcac gtttttcagt ttcatctctt tatccagacc gaacagcccc tggtaaaacg
    18361 atttcgctac ggcctgagat aacgtgtcat tcgcgtcata cggatcgagc gtggtgaaat
    18421 tcgatcctac cgccaccacc acatctttgg cagcgaatgc acaagatgcc atcaacgctg
    18481 tcgcaatgcc cagcgccact aacccactac ggtgtacagc tcttgccatt ttattctccg
    18541 cctgaatgtt atctacgcat gaatgcgtat tctgattgtg gttgtgcgac gtaatgcccc
    18601 ggcccgacgc attgcaacga gacggctgcc acctcttcgc cacgcagatg aatattgctg
    18661 ggaagatcgt ccgacagcag tacacgctgc ggtcgttgtc gggacggttc agcgaccgga
    18721 actgccgcca gtaatttacg cgtataagga tgctgcgggt tttcgaagac cgcgcgccgt
    18781 ggaccaattt caacaatttg cccgagatac atcaccgcca cacgatgact aatccgctct
    18841 accacagcca tatcgtggga gataaacaga tacgcaatgc cgaaatcacg ctggagatcg
    18901 agcaacaagt tgataatctg cccgcgaata gaaacatcca gcgccgaaac ggcttcgtcg
    18961 gcaatgatca cttttggatt caatgccaac gcgcgagcaa tgcagatgcg ctggcgctga
    19021 ccgccggaaa actcatgcgg gtaacgccag gcatgttcag gtaacaggcc cacgcgctcc
    19081 agcaaccacg caacgcgtgc agccgcttct ttacctggca ataaaccgtg tacacgcagc
    19141 ggttcgataa tcgaatcacc gatggtctga cgtgggtcca gcgaagcgta agggtcctga
    19201 aaaataaact gaatatcccg gcgcaatgcc tgaagtttgc cgggtgacaa ggtatcgatt
    19261 cgctgaccgt taaagataat ttcgccgccc tgcgattcga ccaggcgcag caacgcccgc
    19321 ccggtagtgg atttaccgct gccagactcg cccaccagcg atagcgtttc gccaggccag
    19381 agatcaaaac tgactttctc aacggcatgc acttcccgcg ttacgcgatt caacaaaccg
    19441 ctgcgcaaag ggaaacgggt gaccagatta cgcacccgta aaacaggttc accatccacc
    19501 accgttttct gctcgatggg gggggcctgt ttcgctggat gttcaagcga tatcaatggg
    19561 aaacgtcggg gataatctaa ccctttcatc gcaccaagtt gcggaacagc agctaacagc
    19621 gcacgggtgt aaggatgttg cggtgcatga aaaatctgtt cgacggtacc cgtttccacc
    19681 gcctcgccct gatacatcac cagtacccga tcggcaatct ctgccaccac gcccatatcg
    19741 tgagtgataa agataacgcc catcgacatc tctttttgca atactttgat taattgcagg
    19801 atctgcgcct gaatagtgac atccagcgcg gtggttggct catcggcaat cagcaccgcc
    19861 gggcggcatg acagcgccat cgcaatcatc actcgctggc gcatcccgcc agagagttga
    19921 tgcggataac gtgaaagaat ggtttgtgcc tcaggaatgc gtacctgatc cagcatccgc
    19981 ttcgcctcga ccatcgcttc ttcacgactg gcgttctgat gcagacgaat tgattcggca
    20041 atctgttcac ccacagtaaa taccgggttc agcgatgtca tcggctcctg aaatatcatc
    20101 gccatatccg caccgcgaac atggcgcatt tgtgcagcgc tctgctcgct aagttcaatc
    20161 acttcgcgac tgcgccgctg caacagcatt ttatcgcact gtactaaacc gcccgcctgt
    20221 tccaacaggc gcatcaatgc caacgcagtc actgacttac cggagccgga ttcgccaaca
    20281 attgccagcg tctcaccgcg ttgcagacta aaagagagat tgcggaccgc agctattttc
    20341 tgctggtcct gcataaaggc aatattcaga ttttcaaccg ccagcacatt accggcatca
    20401 agttcatcac tgtgtggcaa cggtgtcccc tttttcacgg tagataccgg tggttggcgt
    20461 atcgcctgcg tagccccagg cgcgatacat tccttcggtg ttaaacggta gcgcgacatt
    20521 cccttcatgg tcgatagcga ttaagccacc gctaccgcca agcgcaggga gtttttccat
    20581 tactacccgc tcgcaggctt ccgcgagact taatccgccg taatccatta acgcggcgat
    20641 gtcatatgcc gccagcgcgc ggatgaagac ttcgcccgtg ccggtacaag aaaccgccac
    20701 actggcgtta ttggcgtagc atccggcacc cactaaggga ctatcgccaa ctcgtccggg
    20761 taatttattg gtcattccgc ctgtggacgt ggctgccgcc aaattgccgt ctaaatccaa
    20821 cgccacggcc cccacggtgc ccattttctg tttttcatcc agtggcgcac cgctatggtc
    20881 gaggacggtt gccccttcct tgcgcgctgc cagtagttgt tcataacgca aaggcgtgga
    20941 gaaaatctcc ggcgagacgc gctccatgcc acgagcaaac gcaaaatttt ctgccccttc
    21001 gccaatcatc atcacatgcg ggctttgctc catcaccagc cgggcggcaa gaaccggatt
    21061 acgcagatga ctaacgcccg ccaccgcacc ggctttcagg gtgttaccat ccatcacaca
    21121 ggcgtccagt tcatgggttt catcacgcgt aaagacagcg ccaattccgg cgttaaacag
    21181 tggacactct tccagcagac gcaccgcttc cgtcaccaca tccagcgcac tttcgcccgc
    21241 ttccagcatt ttctgcccgg tttcaacaat ggcagacaac gcctcaatgt agcgtaattc
    21301 ctgttgcaga ctcatctgcg cgcggctaat tgcacctgcg ccaccatgaa ttgcaatgac
    21361 tgctttgccc atagtattcg tccattaagg gtggctcgcc acttttgcta taaatatcag
    21421 aatttttatg aattactcat tcaatttttg aatatagaaa gatggatatg gcatgtaaag
    21481 gcaggagcca acgctacgcc cgaggtggca tacagtctgt ggcgtttttc tgacataata
    21541 ggcaaattcg attttgcctc cgcaggagtg ttttcatgga atttaccacc ggattgatgt
    21601 cgctcgacac cgcgcttaat gagatgcttt ctcgcgtcac cccactgacc gcccaggaaa
    21661 cgctgccact ggtacagtgt tttggtcgta ttctggcgag cgatgtcgtt tcgccgcttg
    21721 atgttccggg gtttgataac tccgcaatgg acggctacgc ggtacgttta gccgatattg
    21781 cctccaggca accgctgccc gttgccggta aatcctttgc cggtcagccg taccatggtg
    21841 aatggcctgc gggtacctgc attcgtatta tgaccggtgc gccggtgccg gaaggctgcg
    21901 aagcggtggt gatgcaggag cagactgaac aaacggacaa tggcgtgcgt tttactgctg
    21961 aagtgcgtag cgggcaaaat attcgccgtc gcggtgaaga tatctctgca ggtgcggttg
    22021 ttttcccggc gggaactcgc ctgactaccg ctgaactgcc agtgattgct tcactgggga
    22081 ttgccgaagt tccggtgatt cgtaaagtgc gtgtagcgct tttttctacc ggtgatgaac
    22141 tccagttgcc cggtcagcca ctgggcgatg gccaaatcta cgataccaac cgtctcgccg
    22201 tacacctgat gttagaacag ttgggatgcg aggtaatcaa cttagggatt atccgcgacg
    22261 atccacatgc cctgcgcgcc gcatttattg aagccgacag ccaggcggat gtggtgatca
    22321 gttccggcgg tgtttcagtg ggtgaagcgg attacaccaa aacgattctt gaagagctgg
    22381 gggagatcgc cttctggaag ctggcgatta aaccaggtaa accgttcgcg ttcggtaaac
    22441 tcagcaatag ctggttctgc ggcctgccgg gcaacccggt ttcagcgacg ctgaccttct
    22501 atcaactggt acagcctttg ctggcaaaac taagcggcaa caccgccagc ggcctgcccg
    22561 cgcgccagcg cgtacgcaca gcgtcccgcc tgaaaaaaac gccaggacgt cttgatttcc
    22621 agcgcggcgt gctgcaacgc aacgccgacg gtgaacttga agtgacgacc accggacatc
    22681 agggttcaca tatatttagc tcctttagtc tcggcaactg ctttatcgtg ctggaacgcg
    22741 atcgcggcaa tgtggacgtg ggcgaatggg tggaagtaga accgtttaac gcgttgttcg
    22801 gaggcctgta atggcggaac tcagcgatca ggagatgctg cgctacaacc ggcaaatcat
    22861 tctgcgcggt tttgattttg acggtcagga ggcgctaaaa gattctcgtg tgctggtagt
    22921 cggcctcggt ggactcggct gcgcagcttc gcagtatctg gcaagcgccg gtgtcggcaa
    22981 cctgacgctg ctcgacttcg acacggtttc gctctcgaat ctgcaacgcc agacgctgca
    23041 cagtgatgct acggtcgggc aaccgaaggt agaatccgcc cgtgacgcca tgacgcggat
    23101 caacccacat atcgcgatta cgccagtcaa tgcactgctt gatgacgctg aacttgctgc
    23161 gatgattgca aaacacgatc tggtactcga ctgtacggat aacgttgcgg tgcgtaatca
    23221 actgaacgct ggctgttttg ccgcgaaggt gccgctggtt tccggcgcgg caattcgtat
    23281 ggaagggcaa atcacggtct tcacttatca ggacggcgaa ccgtgctatc gctgccttag
    23341 ccgtttgttt ggcgagaacg cattaacctg cgtagaagca ggcgtgatgg caccgttgat
    23401 cggcgtaatt ggttcgttgc aggcaatgga agcgatcaaa ctgctggcag gttatggcaa
    23461 accggccagc gggaaaatcg tcatgtacga tgcgatgacc tgtcagtttc gcgaaatgaa
    23521 actgatgcgc aatccggggt gtgaggtgtg cgggcagtaa ttgccacctc caggtcagat
    23581 aagacgcggc actccgccac gtcatcagca atttatgagg atgcagaacg tccggttaaa
    23641 tcgacgttct gccaaacgct cccagccagt cctgttcaaa cttcgccaca gcggcttcca
    23701 ccgccggata gctaatcatc tgttgtgcca catccagtgg cagcgtaatt gattcacatc
    23761 ctgccagtaa gcagtccagc gcctgacgcg gggttttgaa actcgctgcc agcactttcg
    23821 cctgcggcgc atgcattttc aataactggt gtaagtcggt cacagtctga atgccgctac
    23881 cgccctgagc atcaatacga ttaacgtaag gcgcaacata ttccgcacct gccagcgccg
    23941 acagcagccc ttgtgctgcg ccatataccg cggttcccag cgttggaatc ccttccgctt
    24001 ttaacatctt aatagctgcc agcccctcgg cggtcaccgg aactttcacc acgatatccg
    24061 caataataga acgcaattta cgtgcgtcat tcaccatccc ttcagcagtg gtagccatta
    24121 cctgggcaaa cagacgcccc tgaccgccca tcgcttcatg aagttgcgga agcacaacat
    24181 ccagcggttt tttacccgcg gcgataatgc ttgggttagt ggtcacaccc gccagcggaa
    24241 aaatacgtga cagcgccttc accgcaacaa cgtctgaagt atccagatac agttccataa
    24301 ccatcctcaa aatgtacttt ttgtctgaat gtagactatc acgggaaaac tctctgaaca
    24361 gttgacgcac atcaatctaa ctttcattcg aaagtatttt aatgtttgaa cgaaacagag
    24421 aggcaactat gattttcaat attcagcgct actcgaccca tgatggcccc ggtatccgca
    24481 cggtcgtatt tcttaaaggc tgttcgctgg gctgccgctg gtgtcagaac ccggaaagcc
    24541 gcgcccgcac gcaggatctg ctgtatgacg cacgactgtg tctggaaggc tgcgagctgt
    24601 gcgctaaggc cgcgccggaa gtgattgagc gcgcgctgaa tggtttgctt attcatcggg
    24661 aaaagttaac cccggagcat ctgacggcgt taaccgactg ctgtccgaca caggcattaa
    24721 ccgtgtgtgg tgaagtgaaa agcgttgagg agatcatgac gaccgttctg cgcgataaac
    24781 cgttttacga tcgcagcggc ggcggtttaa cgctttcggg tggtgagccc tttatgcagc
    24841 cggaaatggc gatggcgcta ctgcaagcca gccacgaggc aggcattcat actgcggtag
    24901 aaacctgtct gcatgtgccg tggaaatata tcgccccttc tctgccctat atcgatctgt
    24961 ttcttgccga tttaaaacac gttgccgacg cgccgtttaa acagtggacc gacggtaacg
    25021 ccgccagagt gctggataac ctgaaaaaac tcgccgcagc gggcaaaaaa atcattatcc
    25081 gcgtgccgct gattcagggc tttaatgccg acgaaacctc tgtaaaagcc attaccgatt
    25141 ttgccgccga cgagctgcac gttggcgaaa ttcattttct gccctaccac acgctgggca
    25201 tcaacaaata tcacttactt aatctgccct atgacgcccc ggaaaaaccg cttgatgcgc
    25261 cagaactgct cgactttgcc cagcagtatg cctgccagaa agggttaacc gcgaccttac
    25321 gaggataaca accatgacca cactgaaact ggacacgctc agcgaccgca ttaaagcgca
    25381 caaaaatgcg ctggtgcata ttgtgaaacc gccagtctgt accgagcgcg cgcagcacta
    25441 taccgagatg tatcaacaac atctcgataa gccgatcccg gtacgtcgcg cgctggcact
    25501 ggcgcatcac ctggcgaatc gcaccatctg gatcaaacac gatgagttga tcattggcaa
    25561 ccaggcaagc gaagttcgcg ccgcgccgat cttcccggaa tatactgtct cgtggatcga
    25621 aaaagagatt gatgatctgg cagatcgtcc cggtgctggc tttgcggtga gcgaagagaa
    25681 caaacgcgtt ctgcatgaag tgtgcccgtg gtggcgcggt cagaccgtac aggatcgctg
    25741 ctacggcatg tttaccgatg agcaaaaagg tctgctggcg accggaatca ttaaagcgga
    25801 aggcaatatg acctccggcg atgcgcacct ggcggtgaat ttcccgctgc tgctggaaaa
    25861 aggtcttgat ggtctgcgtg agaaagtagc ggaacgtcgc tcgcgcatca atctgacggt
    25921 gctggaagat ttacatggcg aacaattcct gaaagcgatt gatatcgtgc tggtggcagt
    25981 cagtgaacac attgaacgtt tcgctgccct ggcgtgtgaa atggctgcta ccgaaactcg
    26041 cgaaagccgt cgcgatgaac tgctgacgat ggcagaaaac tgcgatctta tcgcccacca
    26101 gccgccgcag actttctggc aggcgctgca actgtgttac ttcatccaat tgattttgca
    26161 gatcgaatct aacggtcact cggtatcgtt tggtcgtatg gaccagtatc tctacccgta
    26221 ctatcgccgc gacgttgaac tcaaccagac gctggatcgc gaacacgcca tcgagatgct
    26281 gcatagctgc tggctgaaac tgctggaagt gaacaagatc cgctccggct cacactcaaa
    26341 agcctctgcg ggaagtccgc tgtatcagaa cgttaccatt ggcggacaaa atctggttga
    26401 tggtcaacca atggatgcag tgaatcccct ctcttacgcg atcctcgaat cctgcggtcg
    26461 cctgcgttcc actcagccta acctcagcgt gcgttaccat gcaggaatga gtaatgattt
    26521 cctcgacgcc tgcgtacagg tgatccgttg cggcttcggg atgccggcgt tcaacaacga
    26581 cgaaatcgtg atcccggaat ttattaaact cggtattgaa ccgcaggacg cttacgacta
    26641 cgcagcgatt ggttgtatcg aaaccgccgt cggtggcaaa tggggctatc gctgtaccgg
    26701 catgagcttt atcaacttcg cccgcgtgat gctggcggcg ctggaaggcg ggcgtgatgc
    26761 caccagcggc aaagtgttcc tgccacaaga aaaagcgttg tcggcaggca actttaacaa
    26821 tttcgatgaa gtaatggacg cgtgggatac gcaaatccgt tattacacgc gtaaatcaat
    26881 cgaaatcgaa tatgtcgtcg acaccatgct ggaagagaac gtacacgata ttctctgctc
    26941 ggcgctggtg gatgactgta ttgagcgagc gaaaagtatc aagcaaggcg gcgcgaaata
    27001 tgactgggtt tctggcctgc aggttggcat tgccaacctc ggcaacagcc tggcggcagt
    27061 gaagaaactg gtatttgaac agggcgcgat tggtcagcaa cagctggctg ccgcactggc
    27121 ggatgacttc gatggcctga ctcatgagca actgcgtcag cggctgatta acggtgcgcc
    27181 gaagtacggc aacgacgatg ataccgtcga tacgctgctg gctcgcgctt atcagaccta
    27241 tatcgacgaa ctgaaacagt accataaccc gcgctacggt cgtggtccgg ttggcggcaa
    27301 ctattacgcg ggtacctcgt ctatctccgc taacgtaccg tttggcgcgc aaactatggc
    27361 aacaccggac gggcgtaaag cccacacccc gctggcagaa ggcgctagtc cggcctctgg
    27421 tactgaccat ctcggcccta ctgcggtcat tggctcggtg ggtaaactgc ctacggcagc
    27481 gattctcggc ggcgtgttgc tcaaccagaa actgaatccg gcaacgctgg agaacgaatc
    27541 tgacaagcag aaactgatga tcctgctgcg caccttcttt gaagtgcata aaggctggca
    27601 tattcagtac aacatcgttt cccgcgaaac gctgctggag gcgaaaaaac atccggatca
    27661 gtaccgcgat ctggtagtgc gtgtcgcggg ctattccgcc ttcttcaccg cgctctctcc
    27721 agacgcacag gacgatatca tcgcccgtac tgaacatatg ctgtaatctc ctcggcccgg
    27781 cggcgtcgtc gccgggctaa atcattcaca tcatcaattt catccttact ttcattcgaa
    27841 atataatttg tgctctgcgt cacattgtta ttacactgtt acggtttcat tttcgagcca
    27901 ggagcacacc tatgagcgta aaagttatcg tcacagacat ggacggtact tttcttaacg
    27961 acgccaaaac gtacaaccaa ccacatttta tggcgcaata tcaggaactg aaaaagcgcg
    28021 gcattaagtt cgttgtcgcc agcggtaatc agtattacca gcttatttcg ttctttcctg
    28081 agctaaagga tgagatctct tttgtcgcgg aaaacggcgc actggtttac gaacatggca
    28141 agcaactgtt ccacggcgaa ctgacccgac atgaatcgcg gattgttatt ggcgagttgc
    28201 taaaagataa gcaactcaat tttgtcgcct gcggtctgca aagtgcatat gtcagcgaaa
    28261 atgcccccga agcatttgtc gcactgatgg caaaacacta ccatcgcctg aaagctgtaa
    28321 aagattatca ggagattgac gacgtactgt tcaagttttc gctcaacctg ccagacgaac
    28381 aaatcccgtt agtgatcgac aaactgcaca tagcgctcga tggcattatg aaacccgtca
    28441 ccagtggttt tggctttatc gacctgatta ttcccggtct acataaagca aacggtattt
    28501 cgcggttact gaaacgctgg gatctgtcac cgcaaaatgt ggtagcgatt ggcgacagcg
    28561 gtaacgatgc ggagatgctg aaaatggcgc gttattcctt tgcgatgggc aatgctgcgg
    28621 aaaacattaa acaaatcgcc cgttacgcta ccgatgataa taatcatgaa ggcgcgctga
    28681 atgtgattca ggcggtgctg gataacacat ccccttttaa cagctgacct ctctaccgga
    28741 gccgtgcgct ccggttttcc cttcttttta catcctgtac atcacatcaa atcattaaac
    28801 ttgcattaa
//
LOCUS       DAFNBJ010000053        28324 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000053 DAFNBJ010000000
VERSION     DAFNBJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.053"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..85)
                     /locus_tag="L2X49_002137"
     CDS             complement(<1..85)
                     /locus_tag="L2X49_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBN4797219.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELEN"
     gene            complement(370..1074)
                     /gene="aat"
                     /locus_tag="L2X49_002138"
     CDS             complement(370..1074)
                     /gene="aat"
                     /locus_tag="L2X49_002138"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBN4797220.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1116..2837)
                     /gene="cydC"
                     /locus_tag="L2X49_002139"
     CDS             complement(1116..2837)
                     /gene="cydC"
                     /locus_tag="L2X49_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBN4797221.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(2838..4604)
                     /gene="cydD"
                     /locus_tag="L2X49_002140"
     CDS             complement(2838..4604)
                     /gene="cydD"
                     /locus_tag="L2X49_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBN4797222.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(4727..5692)
                     /gene="trxB"
                     /locus_tag="L2X49_002141"
     CDS             complement(4727..5692)
                     /gene="trxB"
                     /locus_tag="L2X49_002141"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBN4797223.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6236..6730
                     /gene="lrp"
                     /locus_tag="L2X49_002142"
     CDS             6236..6730
                     /gene="lrp"
                     /locus_tag="L2X49_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBN4797224.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            6865..10971
                     /gene="ftsK"
                     /locus_tag="L2X49_002143"
     CDS             6865..10971
                     /gene="ftsK"
                     /locus_tag="L2X49_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBN4797225.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11126..11737
                     /gene="lolA"
                     /locus_tag="L2X49_002144"
     CDS             11126..11737
                     /gene="lolA"
                     /locus_tag="L2X49_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBN4797226.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            11748..13091
                     /gene="rarA"
                     /locus_tag="L2X49_002145"
                     /gene_synonym="mgsA"
     CDS             11748..13091
                     /gene="rarA"
                     /locus_tag="L2X49_002145"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBN4797227.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13182..14474
                     /gene="serS"
                     /locus_tag="L2X49_002146"
     CDS             13182..14474
                     /gene="serS"
                     /locus_tag="L2X49_002146"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBN4797228.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            14713..17157
                     /gene="dmsA"
                     /locus_tag="L2X49_002147"
     CDS             14713..17157
                     /gene="dmsA"
                     /locus_tag="L2X49_002147"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HBN4797229.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPQWASQITGIPAEKIIQLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNTGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17168..17785
                     /gene="dmsB"
                     /locus_tag="L2X49_002148"
     CDS             17168..17785
                     /gene="dmsB"
                     /locus_tag="L2X49_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBN4797230.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            17787..18650
                     /gene="dmsC"
                     /locus_tag="L2X49_002149"
     CDS             17787..18650
                     /gene="dmsC"
                     /locus_tag="L2X49_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HBN4797231.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(18686..19312)
                     /locus_tag="L2X49_002150"
     CDS             complement(18686..19312)
                     /locus_tag="L2X49_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBN4797232.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            19626..20774
                     /locus_tag="L2X49_002151"
     CDS             19626..20774
                     /locus_tag="L2X49_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4797233.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            20984..22414
                     /locus_tag="L2X49_002152"
     CDS             20984..22414
                     /locus_tag="L2X49_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBN4797234.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGRGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYSMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(22415..23323)
                     /locus_tag="L2X49_002153"
     CDS             complement(22415..23323)
                     /locus_tag="L2X49_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4797235.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            23423..24013
                     /locus_tag="L2X49_002154"
     CDS             23423..24013
                     /locus_tag="L2X49_002154"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HBN4797236.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(24095..24835)
                     /gene="pflA"
                     /locus_tag="L2X49_002155"
     CDS             complement(24095..24835)
                     /gene="pflA"
                     /locus_tag="L2X49_002155"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBN4797237.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(25027..27309)
                     /gene="pflB"
                     /locus_tag="L2X49_002156"
     CDS             complement(25027..27309)
                     /gene="pflB"
                     /locus_tag="L2X49_002156"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBN4797238.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQTDLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(27364..28221)
                     /gene="focA"
                     /locus_tag="L2X49_002157"
     CDS             complement(27364..28221)
                     /gene="focA"
                     /locus_tag="L2X49_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBN4797239.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVIQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         7007 a         7009 c         7407 g         6901 t
ORIGIN      
        1 cgttttctaa ctctacgcgg aacatggtat taggcaacgt ttcaagaacg gtaccttgca
       61 tttcaatatt gtcttctttg gccatctaat cctctggggt atcactaccg taatttgaac
      121 cggcaagata atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa
      181 acaggttcgg cacattactc cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat
      241 aagggagcgg tgagataaac gatgggcgtt acctgacgcg aaaaaatcct tatcggcagc
      301 ggggtaatga gcgtaaccaa ctctgcgacc gcaattataa cactctgggg agaaatgtgc
      361 cgaaaacatt cattcttgtg gtgaaaacaa gcatcgtggt acccagaaat tattcggcaa
      421 tcgtccgagg cgcatttgat tgagataatt aaggtaatcc cggcggggaa tttcgcaggc
      481 accaagcgat gctgtgtgat cgttaaggac ctggcagtcg ataagcttac cgccatgacc
      541 gataaattcc tcacagaata ccagaagcgc cgttttagac gcattttcca tccggctgaa
      601 catggactcg ccacaaaata gcgttccctg ggccacgccg tacataccgc cgacaagctc
      661 atcttcacgc cagacttcaa tggagtgggc atggccgagt tcgtgaaggc gatggtaggc
      721 ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac agccttcaat
      781 gacctgacca aaagcgtaat tcatcgtgac acgatagggc gagcgtttat gaaatcgctt
      841 catactacgg ctgatatgca gtgattctgg ccatagcacc gcgcggggat cgggcgacca
      901 ccagaggatg gggtcgcctg gagaaaacca cggaaaaata ccacgctggt aagccattaa
      961 caggcgcgca gggctaagat cgcccccaag tgccagcagg ccgttaggct cacgtaatgc
     1021 gccttccggg gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc
     1081 aaaactccac gcaagtcgga tcgttcaata atagcttaca aaccctgctt gaactggtaa
     1141 taacgcccct gtctggcaag cagctctgcg tgagtacctt gctcaataat ttgcccgttg
     1201 tccatcacta ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt gaccattaac
     1261 accgttttct cacgcatcat ttctgcaagc aattcaagga tctggctttc ggttgtggca
     1321 tctaagcctt cggtaggttc atccagcaac accagtggcg catcatgtaa cagcgcacgg
     1381 gcgatagcca gacggcgcag ttcaccaccg gagagctggc gtccgccttc acctaaccaa
     1441 ctgttgagac ctgcatcctc gagcagcttc tccagaccaa cgcgacgcaa gctctccgcc
     1501 agagcctcat cactactgcc aggcgaggcg agtaacagat tatcacgcag cgtggcgcta
     1561 aacagatgca ctcgctgagg aacaacgctg atggtctgtc gtagagccgc ttcattcagg
     1621 ctggctatgg ggctatcgtt aagcaaaatc tctccctgtt gcgggtccca agcgcgggtc
     1681 agcagttgta acagtgttga tttgccgcat ccggttcgcc cgagaatcgc aatatgttcc
     1741 ccggcgttta cctgaagaga aatcccttta agtgcctgtt gggattgctc cggataagtg
     1801 aactgaacat cccgtaatgt cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg
     1861 aaggtgactt ccggtttttg atccgttaaa tcagtgatcc gaacggcaga ggcaatgact
     1921 tgccccagat gctgaaatgc acccgttact ggtgccagtg cttcaaacgc ggctaacgcg
     1981 cagaagacaa acagggcaat taacgcgccg ggttgagcat tgccgccaac gccgccagac
     2041 gccatccaca gcatcaggat caccgctaac gcgccaatga gcagcattat cgcttgcgac
     2101 aatgcggtca gttcagattg acggcgttgc gcttccagcc attgaatttc tgtattctct
     2161 agttgcgtgc gataacgatc gctggcgcca aaaatggtca gctcagcttg cccttgcagc
     2221 caggccgtca gttgttggcg atactgtccg cgaagatgag tcagattttg cccggtgctt
     2281 tttcccgcac gataaaacag cggtggcatc aggaaaagcg tcagtaacat aatgccgccc
     2341 agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga ttgtcaccac cataatcacc
     2401 acaaaagcgc ccaccagcgg cgagataacg cgcagataaa gatgatcgag cgtatcaaca
     2461 tccgccacca cgcgattgag caattcgccc tgacgatagc gcgccagtcc ggcaggggag
     2521 aggggcagca atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg gaaagtcgcg
     2581 tcgtgactta ccagacgttc aaaataacgc ccggcagtac gggtgattgc tgcgccacgc
     2641 acgcccgcag cgggtagcat atagttgaag ctgtacagtc cggcaacccc cgcaaccgct
     2701 gaggccgaga ggaaccagcc ggaaagtgtc aacagaccga tactggcgag cagcgtgaca
     2761 attgccagca cgataccaag acttaacatc catttatgac gtttatacag tgccagatag
     2821 ggtagcaaag cgcgcattta aatctcctcc tgacgatggg ccagtaatgt ggcgaatggg
     2881 ccaccagcca cacttaattc cgcgtaacgt ccttgctcaa taatccggcc atcctgcata
     2941 acccaaatga catcccagtc agcaagatct tctaactggt gggtgaccat taacgttgtc
     3001 tggcgcagag aggcggcatt cagcgcctcc attacgcgct gttcactgtg agcatcaagg
     3061 ctggcagcgg gttcatccaa cagtaatagc gaacagggat ttagtaacgc acgggccacc
     3121 gccacgcgct gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac aggtgtatca
     3181 acgccttgtg gcaggagcgg tagaaactcg ctgacccagg cgttatccag cgctgcttgt
     3241 aattcttgtt cgctggcatc aggtcgcgcc agtagtacgt tatcccgcaa tgttgctgcc
     3301 ggtaattgtg ggttttgccc aacccaggag agatgtttac gccatgattc tggtgataaa
     3361 tcgcgtaatt ctatcccgtt gattcgtaat gatccctgat atgagagaaa accagaaagc
     3421 gcgttcagca gtgagctttt acctgaaccg ctgcgaccaa ccaacactgc acgttggcct
     3481 gctggcaaag taaagttcag cggtccggcc agcgttttac cttccggcga cgtgataaac
     3541 agatcctcgg cctcaatggt caccggatcg gtcgatgcta attccacctc gccacgctgc
     3601 ggatgggcga gcggggtttc cataaacgtt ttcagactgt cagctgcacc aacagcctgg
     3661 gctttagcat gataaaacgt accgagatcg cgcaatggct ggaaaaactc tggcgcaagg
     3721 atcagggcca gaaaacccgc agccagcgtc acaccggtat cgtagtgacc aaaatccagc
     3781 tcgccgagat aggaaaaacc aaagtagacc gccaccagag caattgacag cgaggtaaaa
     3841 aattcgagaa tgccggagga taaaaacgcc agccgtagca cttccattgt ccgttggcgg
     3901 aaatcttccg aagcagaacg aatactttca atttcagctt caccacgacc aaaaatacgc
     3961 aatgtttcca tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga
     4021 aagttacgcc ggttagcatc ggcagccccc atcccaacca gcgccataaa caacggaatt
     4081 aacggtgctg tgcccagcag aatgagcgcc gcagcccagt tagaggggaa gatcgccacc
     4141 acaatcagca acggcaccga cactgccagc gccatttgcg gcaggtagcg tgcatagtaa
     4201 tcatgcatat cgtcaatttg ctcgagtacc aacgtcgccc agctccccgc aggtttaccc
     4261 tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag
     4321 cggatatgct gcccggcgtg ataacccacc cgttcgcgta accagaccac ccatgcgcgc
     4381 agtacaaagg tcagaaccag taacgtaaag ggaagcagca gggcttcacg gggaatattc
     4441 tccataatca tatgttgcag aattcgcgcc atgaaccagg cctgggcaat gatcaatatg
     4501 ccgcttacaa agcccagcag acgagaaata ttcagccaac gttgggagat gacgctttgc
     4561 tgttttaacc agcgggttaa ctctttttga cgggatttat tcattgcacg cttagcaggt
     4621 gagttatcag aattatttgc agagcaatgt tacaacgggg aaaaaataaa ggcgacccat
     4681 agtcgcatgg tgtcgccttc tttacttttg ttactgattt gtaaaattat tttgcgtcag
     4741 ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat gcagcctgta ccggccgaag
     4801 taatggcctg acgataaata tgatccatca cgtcacccgc ggcaaagacg ccaggaatgc
     4861 tggtctgggt ggcattacca tgaatacccg actgcacttt gatgtagccg ttttccagtt
     4921 ccagctgccc ttcgaaaatg gcagtattcg ggctgtgacc gatagcaaca aacagaccag
     4981 caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga acgccagtga
     5041 cacccatttg atcgccggtc acttcttcca gcgtacggtt ggtgtgcaga atgatgttgc
     5101 cgttctccac tttatccatc agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac
     5161 ggcggtgaat aagatgcact tccgaagcga tgttagacag atacagcgcc tcttcaaccg
     5221 cggtattgcc gccgccgatg accgcaactt tctggttgcg atagaagaaa ccgtcgcagg
     5281 ttgcacaagc agaaacccca cggcctttaa aggcttcttc agagggcagg ccgagatagc
     5341 gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt gtattcgccg ttatcgccat
     5401 tcagacggaa cggacggttt tgcagatcca ccttgttgat atgatcaaaa atgatctcag
     5461 tttcaaactt ggtggcatgt tcgtgcatgc gctccattaa taacggaccg gtcagatcgt
     5521 ttggatcgcc aggccagttt tccacttccg tggtggtggt cagttggccg cctttttcca
     5581 tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc gtagacagca gcggtgtatc
     5641 ccgccgggcc tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc atgagatccc
     5701 catagttgtt ggcagacaat gggcaggatt gtagggaatt tacagacgta aaaaaagagt
     5761 atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc
     5821 cgtcattatc acctctgcta cttaaatttc ccgctttata agccgattaa atgatgaata
     5881 aacgcccctg ttaatgaata tctggcatgt tgtactaaaa atcgatgttt tgctttgaca
     5941 atcccctggt gttttgcgaa aacattcgag gaagaaaaaa acagtattct tatatgcgca
     6001 taaccatgca tgtaaatacc atgtttaccg tgctagtgaa atctacgtat ggcgtggaca
     6061 gacgccattc gtgatgtcga tagctgccac aaggcaacgg tcttctcacc gtagacccag
     6121 gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt gacgggtagc gactctgaac
     6181 agtgatgttt cagggtcaga caggagtagg gaaggaatac agagagacaa taataatggt
     6241 agatagcaag aagcgccctg gcaaagatct cgaccgtatc gatcgtaaca ttcttaatga
     6301 gttgcaaaag gatgggcgta tttctaacgt cgagctttct aaacgtgtgg gactttcccc
     6361 aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa gggtttattc agggctatac
     6421 ggcgctgctg aacccccatt atctggatgc atcacttctg gtattcgttg agattactct
     6481 gaatcgtggc gcaccggatg tgtttgaaca attcaatacc gctgtacaaa aacttgaaga
     6541 aattcaggag tgtcatttag tatctggtga tttcgactac ctgttgaaaa cacgcgtgcc
     6601 ggatatgtca gcttaccgta agttgctggg ggaaaccctg ctgcgtctgc ctggcgtcaa
     6661 tgacacacgg acatacgttg ttatggaaga agtcaagcag agtaatcgtc tggttattaa
     6721 gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt gattacactc ctgttaatcc
     6781 atacagcaac agtactgggg taacctggta ctgttgtccg ttttagcatc gggcaggaaa
     6841 agcctgtaac ctggagagcc tttcttgagc caggaataca ctgaagacaa agaagtcaca
     6901 ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt tgctgatcct tattgtcctg
     6961 tttgccgtct ggttgatggc tgccttacta agctttaacc cttcggaccc cagctggtcg
     7021 caaacggcct ggcatgaacc tatccataat ttaggtggga tgcccggtgc gtggttggca
     7081 gatacgctgt tctttatttt tggcgtgatg gcttacacca ttcccgtcat tattgtcggc
     7141 ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat acattgatta ttttgccgtt
     7201 tcgctacgca tcattggcgt tttggcgctc atccttacct cctgtggtct ggcggcaatc
     7261 aacgctgacg atatctggta ttttgcctcc ggtggcgtca ttggcagctt actaagtact
     7321 acgctacaac cactgctaca cagtagcggg ggaactattg cgctgctctg cgtttgggct
     7381 gcgggcctga cgctgttcac cggttggtca tgggtgacca ttgctgaaaa actcggcggc
     7441 tggattttaa acattctcac cttcgccagt aatcgtaccc gtcgcgatga tacctgggtc
     7501 gatgaagatg aatatgaaga tgacgaagag tatgaagatg aaaaccacgg caaacagcat
     7561 gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc gtcgtaaacg gttggcggaa
     7621 aaattcatta atccgatggg gcggcaaaca gacgctgcgt tgttctccgg taagcggatg
     7681 gatgatgacg aagagattac ctacactgca cgcggtgtgg ctgccgaccc ggacgacgtc
     7741 ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg aatacgatcc attattaaac
     7801 ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg ctaccacggc gacacaaagc
     7861 tgggctgcgc cggttgaacc tgtgactcag acgccgcctg ttgcctctgt tgatgttcca
     7921 ccctcgcaac ctacagtagc ctggcagcct gtaccgggtc cacaaacggg agagccggtt
     7981 attgctcctg caccggaagg ttacccacag cagtcacaat atgcgcagcc tgcagtgcaa
     8041 tataatgagc cgctgcaaca accagtacag ccgcagcagc cgtattatgc acctgcagct
     8101 gaacaacctg cgcaacaacc ttactacgct cctgcggcgg aacaacctgt tcagcaaccg
     8161 tattacgcaa ctgcgccgga acagcctgca caacagccgt attacgcccc tgcgccagaa
     8221 caaccggtgg caggtaacgc ctggcaagcc gaagagcagc aatccacttt tgctccacag
     8281 tctacatacc agactgagca aacttatcag caaccagccg ctcaggagcc gttgtaccaa
     8341 cagccgcaat ccgttgaaca gcagcctgtt gtggagcctg aacccgttgt agaagagaca
     8401 aaacccgcgc gtccgccgct ttactatttt gaagaagtgg aagagaagcg agcccgtgaa
     8461 cgtgaacaac ttgcggcctg gtatcaaccg attccagaac cggttaaaga accagaaccg
     8521 atcaaatctt cgttgaaagc gccttctgtt gcagcagtac ctccagtaga agccgctgcc
     8581 gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac tggcgacggg ggctgccgca
     8641 accgttgccg cgccagtctt cagtctggca aatagcggtg gaccgcgtcc gcaagtcaaa
     8701 gaggggattg gcccgcagtt gccacgaccg aaacgtatcc gcgtgccaac tcgtcgtgaa
     8761 ctggcgtctt acggtattaa gctgccctca cagcgtgcgg cggaagagaa agcccgtgaa
     8821 gcccagcgca atcagtatga ttctggcgat cagtataacg atgatgaaat cgatgcgatg
     8881 cagcaggatg aactggcacg tcagttcgcc cagacacagc agcaacgcta tggcgaacag
     8941 tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg ctgcggcaga ggctgaactg
     9001 gctcgtcagt ttgcgcaaac tcaacaacaa cgttattccg gcgaacaacc ggctggggcg
     9061 aatccgttct cgctggatga ttttgaattt tcgccaatga aagcgttgct ggatgatggt
     9121 ccacacgaac cgttgtttac gccaattgtt gaacctgtac agcagccgca acaaccggtt
     9181 gcaccgcagc agcagtatca gcagccgcaa caaccagttc cgccacagca gcaatatcag
     9241 cagccgcaac agccggttgc gccgcagccg cagtatcagc agccacaaca gcaggttgcg
     9301 ccgcagccac aatatcagca gccgcaacaa ccggttgcgc cacagccgca atatcagcag
     9361 ccgcaacaac cagttgcgcc gcagccgcag tatcaacagc cgcaacaacc ggttgcaccg
     9421 cagcagcagg ataccctgct tcatccgctg ttgatgcgta atggcgacag ccgtccgttg
     9481 cataaaccga cgacgccgct gccttctctg gatttgctga caccgccgcc gagcgaagtg
     9541 gagccggtag atacctttgc gcttgaacaa atggctcgcc tggtggaagc gcgtctggct
     9601 gatttccgta ttaaagccga tgtcgtcaat tactctccgg ggccggttat cactcgcttt
     9661 gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt ctaacttgtc acgggacctt
     9721 gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag ttattcctgg caaaccctat
     9781 gtaggtctgg agttaccaaa taaaaaacga caaaccgttt atctgcgcga agttttggat
     9841 aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg tgctgggtaa agatatcgcc
     9901 ggtgagccgg tggttgccga tctggcgaaa atgccgcact tgttggttgc ggggactacc
     9961 ggttccggta aatctgtcgg tgtgaacgcg atgatcctga gcatgcttta taaagcacag
    10021 ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc tggagctttc ggtttatgaa
    10081 ggcattccgc atctgttaac ggaagtcgtt actgatatga aagatgccgc caacgcgctg
    10141 cgctggtgtg ttaacgagat ggagcgtcgt tataaactga tgtctgcgct gggtgtgcgt
    10201 aatctggcgg gttataacga aaaaattgct gaagccgatc gcatgatgcg tccgattcca
    10261 gacccgtact ggaagccggg tgacagtatg gatgcccagc atccggtgct gaaaaaagaa
    10321 ccatacattg tggtgttggt tgacgaattt gccgacctga tgatgacggt aggtaaaaaa
    10381 gtggaagagc tgatagcacg tctggcgcaa aaagcccgtg ccgcgggtat ccacctcgta
    10441 ctggcaactc agcgtccatc ggttgatgtt attactggtc tgattaaagc gaatattccg
    10501 acccgtatcg cctttaccgt atccagtaag attgactcac gtaccattct tgatcaggct
    10561 ggcgcggaat cactgctggg gatgggggat atgctctact ctgggccgaa ctccacgttg
    10621 ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag ttcatgccgt ggtgcaggac
    10681 tggaaagcgc gtggtcgccc acagtatgtt gatggcatca cttccgacag tgaaagcgaa
    10741 ggtggcgcgg gcggtttcga tggcgctgaa gaactggatc cgttgttcga tcaggcggtg
    10801 cagtttgtca ctgaaaaacg caaagcgtca atttctggcg tacagcgtca gttccgcatt
    10861 ggttataacc gtgcagcgcg tattatcgaa cagatggaag cgcaggggat tgtcagcgaa
    10921 caggggcaca acggtaatcg tgaagtgctg gccccaccgc cgtttgacta attaatgcat
    10981 tgccggataa ggcgcggtag cgtcgcatcc ggcactctat caactgaaaa ttcagtattt
    11041 tcttctttcc tcaagctgat tattagcctg gaatagagag tagagggaac tcccgatcgg
    11101 gagtgacgta atttgaggaa taatgatgaa aaaaattgcc atcacctgtg cattactctc
    11161 aagcttagta gcaagcagcg tttgggctga tgccgcaagc gatctgaaaa gccgcctgga
    11221 taaagtcagc agcttccacg ccagcttcac acaaaaagtg actgacggta gcggcgcggc
    11281 ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca aacttattca actggcatat
    11341 gacacaacct gatgaaagca ttctggtttc tgacggtaaa acactgtggt tctataaccc
    11401 gttcgttgag caagctacgg caacctggct gaaagatgcc accggtaata cgccgtttat
    11461 gctgattgcc cgcaaccagt ccagcgactg gcagcagtac aatatcaaac agaatggcga
    11521 tgactttgtc ctgacgccga aagccagcaa tggcaatctg aagcagttca ccattaacgt
    11581 gggacgtgat ggcacaatcc atcagtttag cgcggtggag caggacgatc agcgcagcag
    11641 ttatcaactg aaatcccagc aaaatggggc tgtggatgca gcgaaattta ccttcacccc
    11701 gccgcaaggc gtcacggtag atgatcaacg taagtagagg cacctgagtg agcaatctgt
    11761 cgctcgattt ttcggataat acttttcaac ctctggccgc gcgtatgcgg ccagaaaatt
    11821 tagcacagta tatcggccag caacatttgc tggctgcggg gaagccgttg ccgcgcgcta
    11881 tcgaagccgg gcatttacat tctatgatcc tctgggggcc accgggtacc ggcaaaacaa
    11941 ccctcgctga agtgattgcc cgctatgcga acgctgatgt ggaacgtatt tctgccgtca
    12001 cctctggcgt gaaagaaatt cgcgaggcga tcgagcgcgc ccggcaaaat cgcaatgcag
    12061 gtcgccgcac tattcttttt gttgatgaag ttcaccgttt caacaaaagc cagcaggatg
    12121 catttctgcc acatattgaa gacggcacca tcacttttat tggcgcaacc actgaaaacc
    12181 cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg tgtctatcta ttgaaatccc
    12241 tgagtacaga ggatattgag caagtactaa ctcaggcgat ggaagacaaa acccgtggct
    12301 atggtggtca ggatattgtt ctgccagatg aaacacgacg cgccattgct gaactggtga
    12361 atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat ggcggatatg gccgaagtcg
    12421 atgatagcgg taagcgggtc ctgaagcctg aattactgac cgaaatcgca ggtgaacgta
    12481 gcgcccgctt tgataacaaa ggcgatcgct tttacgatct gatttccgca ctgcataagt
    12541 cggtacgtgg tagcgcaccc gatgcggcgc tctactggta tgcgcgaatt attaccgccg
    12601 gtggcgatcc gttatatgtc gcgcgtcgtt gcctggcaat tgcttccgaa gatgtcggca
    12661 atgccgaccc acgggcgatg caggtggcaa ttgcggcctg ggattgcttt actcgcgttg
    12721 gcccggcgga aggtgaacgc gccattgctc aggcgattgt ttacctagcc tgcgcgccaa
    12781 aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc ggatgctcgc gaacgtccgg
    12841 attatgacgt gccggttcat ttgcgtaatg cgccgacgaa attaatgaag gaaatgggct
    12901 acgggcagga atatcgttac gctcatgatg aagcaaacgc ttatgctgcc ggtgaggttt
    12961 acttcccgcc ggaaatagca caaacacgct attatttccc gacaaacagg ggccttgaag
    13021 gcaagattgg cgaaaagctc gcctggctgg ctgaacagga tcaaaatagc cccataaaac
    13081 gctaccgtta atgttatcgt tgcggtaatg ttgttactgt atccctgtgg tcgcaggctg
    13141 tggccacatc tcccatttaa ttcgataagc acaggataag catgctcgat cccaatctgc
    13201 tgcgtaatga gccagacgca gtcgctgaaa aactggcacg ccggggcttt aagctggatg
    13261 tagataagct gggcgctctt gaagagcgtc gtaaagtatt gcaggtcaaa acggaaaacc
    13321 tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca ggcgaaagcg cgcggggaag
    13381 atatcgagcc tttacgtctg gaagtgaaca aactgggcga agagctggat gcagcaaaag
    13441 ccgagctgga tgctttacag gctgaaattc gcgatatcgc gctgaccatc cctaacctgc
    13501 ctgcagatga agtgccggta ggtaaagacg aaaatgacaa cgtcgaagtc agccgctggg
    13561 gcaccccgcg tgagtttgac tttgaagttc gtgaccatgt gacgctgggt gaaatgcact
    13621 ctggcctcga ctttgcagcc gcagttaagc tgaccggttc ccgctttgtg gtaatgaaag
    13681 ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat gctggatctg cataccgaac
    13741 agcatggcta cagtgagaac tatgttccgt acctggttaa ccaggacacg ctgtacggta
    13801 cgggtcagct gccgaaattt gctggcgatc tgttccatac tcgtccgctg gaagaagaag
    13861 cagacaccag taactatgcg ctgatcccaa cggcagaagt tccgctgact aacctggtgc
    13921 gcggtgaaat catcgatgaa gatgatctgc caattaagat gaccgcccac accccatgct
    13981 tccgttctga agccggttca tatggtcgtg acacccgtgg tctgatccgt atgcaccagt
    14041 tcgacaaagt tgaaatggtg cagatcgtgc gcccggaaga ctcaatggcg gcgctggaag
    14101 agatgactgg tcatgcagaa aaagtcctgc agttgctggg cctgccgtac cgtaaaatca
    14161 tcctttgcac tggcgacatg ggctttggcg cttgcaaaac ttacgacctg gaagtatgga
    14221 tcccggcaca gaacacctac cgtgagatct cttcctgctc caacgtttgg gatttccagg
    14281 cacgtcgtat gcaggcacgt tgtcgcagca agtcggacaa gaaaacccgt ctggttcata
    14341 ccctgaacgg ttctggtctg gctgttggtc gtacgctggt tgcggttatg gaaaactatc
    14401 agcaggctga tggtcgtatt gaagtaccag aagttctgcg tccgtatatg aacggactgg
    14461 aatatattgg ctaataccca atttttctga atctaaaaag cgcctgcggg cgcttttttt
    14521 gtctcccttt gataccgaac aataattact cctcacttac acgtaatact actttcgagt
    14581 gaaaatctac ctatctcttt gattttcaaa ttattcgatg tatacaagcc tatatagcga
    14641 actgctatag aaataattac acaatacggt ttgttactgg aatcaatcgt gagcaagctt
    14701 gagtgagcca ttatgaaaac gaaaatccct gatgcggtat tggctgctga ggtgagtcgc
    14761 cgtggtttgg taaaaacgac agcgatcggc ggcctggcaa tggccagcag cgcattaaca
    14821 ttacctttta gtcggattgc gcacgctgtc gatagcgcca ttccaacaaa atcagacgaa
    14881 aaggttatct ggagcgcctg tacagttaac tgtggtagtc gctgcccgct acgtatgcac
    14941 gtcgtggacg gtgaaatcaa atatgtcgaa acggacaata ccggcgatga caattacgac
    15001 ggcctgcacc aggttcgcgc ctgtctgcgt gggcgttcca tgcgtcgccg tgtctacaat
    15061 ccggaccgcc tgaaatatcc gatgaaacga gtcggggcgc gcggtgaagg caaattcgag
    15121 cgcattagct gggaagaagc ctacgacatc atcgcgacca atatgcagcg cctgatcaaa
    15181 gagtacggca acgagtctat ctatctgaac tatggcaccg gtacgctggg cggcaccatg
    15241 acccgctcct ggccgccggg aaataccctg gtcgcgcgcc tgatgaactg ctgcggcggt
    15301 tatcttaacc attacggtga ctactcttct gcgcaaattg cggaaggttt gaactatacc
    15361 tacggcggct gggcagatgg taacagcccg tcggatatcg aaaacagtaa attggtagtg
    15421 ctgtttggta ataacccagg cgaaacgcga atgagtggcg gtggggtgac ttactatctt
    15481 gaacaggcac gccagaaatc taatgcccgc atgatcatca tcgatccgcg ctataccgac
    15541 accggtgccg ggcgcgaaga tgagtggatc cctattcgtc caggaacaga tgccgcactg
    15601 gttaacggtc tggcgtatgt catgatcacc gaaaacctgg tggatcaggc attccttgat
    15661 aaatattgcg ttggctacga tgagaaaacc ctgcccgcta gcgcgccgaa aaatggccac
    15721 tataaagctt atattctggg tgaagggcca gatggcgtgg ctaaaacacc gcaatgggcc
    15781 tcgcaaatca ccggtatccc ggcagagaaa atcattcaac tggcacggga aatcggcagt
    15841 accaaaccgg cgtttatcag ccagggatgg gggccacagc gccacgccaa cggcgaaatc
    15901 gcgactcgcg ctatctcgat gctggcgatt ctgaccggta acgtcggcat taacggcggt
    15961 aacaccggcg cgcgtgaagg ttcctatagc ctgccgtttg tccgtatgcc gaccctcgaa
    16021 aacccgatcc agaccagcat ttctatgttt atgtggaccg atgccattga acgtggcccg
    16081 gaaatgacgg cgctacgtga tggtgtgcgc gggaaagata agctggatgt gccgatcaaa
    16141 atgatctgga actatgccgg taattgcctg attaaccagc attctgaaat caaccgtacc
    16201 catgaaatcc ttcaggatga taagaagtgc gagctgattg tggttatcga ctgccacatg
    16261 acctcatcgg cgaaatatgc tgacatcctg ctgcctgact gcaccgcttc cgaacagatg
    16321 gactttgcac tggatgcatc ctgcgggaat atgtcttacg tgattttcaa cgatcaggtg
    16381 attaaaccgc gctttgaatg taaaaccatc tatgaaatga ccagcgaact ggcaaaacgt
    16441 cttggcgttg agcaacagtt tactgaaggc cgtacacagg aagagtggat gcggcatctg
    16501 tatgcccagt cgcgggaagc gattcctgaa ctgccaacgt ttgaagagtt ccgcaagcag
    16561 gggatcttta aaaagcgcga cccacaaggg catcacgttg cttataaagc cttccgtgaa
    16621 gatccgcagg caaacccact gactacgcca tcgggcaaaa ttgagattta ttcgcaggcg
    16681 ctggctgaca ttgccgctac ctgggaattg ccagaaggcg atgtgatcga tccactgccg
    16741 atctacacgc cgggctttga aagttatcag gatccgctga acaaacagta tccgctgcag
    16801 cttacgggtt tccactataa atctcgcgtt cactcaactt acggcaacgt tgatgtgctg
    16861 aaagcggctt gccgtcagga aatgtggatc aacccgcttg atgcgcaaaa acgcggtatc
    16921 aacaacggcg ataaagtcag gatctttaac gatcgtggtg aagttcatat tgaggcgaaa
    16981 gtgacgccac gaatgatgcc gggtgtggtc gcactgggtg aaggtgcctg gtatgacccg
    17041 gatgcaaaac gtgtcgataa gggcggttgt attaacgtac tgaccactca acgtccgtct
    17101 cctctcgcta aggggaatcc gtcacataca aaccttgttc aggttgaaaa ggtgtaagga
    17161 gtaaccgatg acaacccagt atggattttt tattgattcc agccgttgca ccggttgcaa
    17221 aacctgcgag ctggcctgta aagactacaa agatttgacg ccagaagtca gcttccgccg
    17281 catttatgaa tatgctggcg gcgactggca ggaagataac ggtgtctggc accagaacgt
    17341 gtttgcctac tatctgtcga tttcatgtaa ccactgcgaa gatccggctt gtactaaagt
    17401 ctgcccgagc ggtgcgatgc ataaacgcga agatggtttt gttgtggtcg atgaagatgt
    17461 gtgcattggc tgccgctact gccatatggc ttgcccgtat ggcgcaccgc aatataacga
    17521 aacgaaaggc catatgacca aatgcgatgg ttgttatgac cgtgttgcgg agggtaaaaa
    17581 gccgatctgt gttgaatctt gtccgctgcg ggcgctggat ttcggcccta tcgacgagct
    17641 gcgtaaaaaa catggcgatc tggcggccgt tgcgccgttg ccgcgagctc actttaccaa
    17701 accgaatatt gtgatcaaac ccaatgccaa tagccgcccg accggggata ccactggcta
    17761 tctggcaaac ccgaaggagg tgtgagatgg gaagtggatg gcatgaatgg ccgctgatga
    17821 tcttcacggt cttcgggcaa tgtgtagcag gtggttttat cgttctggct ttggcgctgc
    17881 tcaaaggcga cctgcgagca gaagcccagc agcgtgttat cgcctgcatg tttggtttat
    17941 gggtgctgat gggcattggc tttatcgcct ctatgctcca tcttggttca ccaatgcgcg
    18001 cttttaactc gctcaaccgg gtaggggctt cagcactcag taacgaaatc gccagcggtt
    18061 cgatcttttt tgccgtaggc ggcatcggct ggttgctggc aatgctgaaa aagctgtcac
    18121 cggcattgcg tacgctgtgg ctgatagtga cgatggttct tggcgtcatc tttgtctgga
    18181 tgatggtgcg tgtgtataac agcattgata ccgttccgac ctggtacagc atctggacgc
    18241 cgatgggctt cttcctgacg atgtttatgg gcggcccgtt actgggttac ctgttgttga
    18301 gtctggctgg cgtcgatggc tgggcgatgc gtctgctgcc agcaatttct gtactggcac
    18361 tggtagtgag tggcgtggtg tcagtgatgc agggcgcaga gctggcgact attcatagtt
    18421 ctgtgcagca ggccgcagcg ctggtgccgg actatggtgc gctgatgtcc tggcggatcg
    18481 tgcttttggc cgttgccctg tgcttgtgga ttgcaccaca gctaaaaggt tatcagcctg
    18541 cggttccgct actatccgtc tcattcattc tgcttctggc aggggaatta atcggtcgcg
    18601 gcgtattcta tggtttgcat atgaccgtgg ggatggccgt cgcaagctaa tcagtaacaa
    18661 ccggggtttc ggccccggtt ctcttttatt tctgcttcgt taacgtgtca taactggtca
    18721 tcaaattacg ataatcagga atgtggttag agaacaacgt cgccagtcct tcaatatcat
    18781 tacgccagtc gcgatgcagc tcgcacgcca cgccgaacca tgtcatcaat tgcgcaccag
    18841 cttgcgacat gcgatcccat gccgaatgcc gggtaatttc attaaaggtc cctgacgcgt
    18901 cagtcacgac aaagacgtca aacccttctt caatggctga aagcgccggg aatgccacgc
    18961 aaacttcggt taccacaccg gcaataatta actgtttttt acctgtcgct ttgacagctt
    19021 ttacaaaatc ttcgttatcc caggcgttaa tatttcccgg gcgagcaatg taaggtgtat
    19081 cggggaattg tgctttcagt tctggaacta atgggccgtt aggaccggtt tcaaaactgg
    19141 tggtaagaat agttggtaag ttgaaatact tagccaggtc acccagcgcc agcacgttat
    19201 ttttaaactt atcaggttcg atatcccgta caagggaaag taaaccagcc tggtgatcaa
    19261 caagcaaaac ggcagcatca tttttatcaa gacgaacata cggtttggtc atcctcttct
    19321 cctttcgaga gtcagtggta cgtctgaaat cgatcagacg cgatgcattg ctctgaaagc
    19381 atagacggga aatatgagtt tgctgtgacc atgaaatttt tcgactgaac gaagcgtatg
    19441 aaatttgtgt tagttcaata aaaacaatca gatgagacta atcatctctt cagcattagc
    19501 gttatgaatg agtaatataa atcgataata ccgccgctgg gatatcgcgt attttcaccc
    19561 attgacaatg tttttggcgg tggcatgatg cgcatgaaat ttgaacttcc tcacggtttt
    19621 aattcatgtc cacgtatacc cggcctgtca tgcttttgct gtctggcctg cttttgttga
    19681 ctctggcgat tgcggtgtta aatacactcg tgccgctttg gctcgcccag gaacacatgt
    19741 ccacatggca ggtaggcgtt gtcagctcat cctattttac cggcaacctt gtcggtacat
    19801 tgctgacagg gtatgtcatt aagcgcattg gctttaaccg cagctattat ctggcctcct
    19861 tcatttttgc cgctggctgt gccggccttg gcctgatgat tggattctgg agctggttgg
    19921 cttggcgttt tgtcgcgggc gtcggctgtg ccatgatttg ggtggttgtt gagagcgcgc
    19981 tgatgtgcag tgggacgtca cgtaaccgtg ggcgtttgct tgctgcgtat atgatggttt
    20041 attacgtggg aacgttttta ggccagttac tggtcagcaa agtttcaacc gagctgatgt
    20101 ccgtattgcc gtgggttaca ggtttgacgt tggcagggat cttaccgctg ttgtttacgc
    20161 gtgtgctgaa tcagcaggct gaaaaccatg attcgacgtc aattacgtca atgctaaaac
    20221 tccgtcaggc gcggcttggc gtgaatggct gcattatctc aggaatcgtt ctgggatctc
    20281 tatatggcct gatgccgctg tacctcaatc acaaaggggt gagcaatgcc agcattggtt
    20341 tctggatggc ggtactggtc agtgcgggta tccttggaca atggccgatt ggacgtctgg
    20401 cggataagtt tggtcgactg ctggtgttgc gtgttcaggt ctttgtcgtc attctcggca
    20461 gtatcgcgat gcttagccag gcggcgatgg ccccagcgtt attcatcctc ggtgccgctg
    20521 gctttacgct atatccggtg gcgatggcat gggcttgcga gaaagttgaa catcatcaac
    20581 tggtggcgat gaaccaggcc ttactgttga gctatactgt gggaagtctg cttggcccgt
    20641 catttaccgc tatgctaatg cagaatttct ccgataattt attgtttatc atgatcgcca
    20701 gcgtatcgtt tatctatttg ctgatgctgc tgcgcaacgc cggtcatacg ccgaaacccg
    20761 ttgctcacgt gtaaatgaat tcaagcagag tgtgaactta ctgtttcaca ctctgctttt
    20821 ttgtttcttc tatctgactt gctttattcc aaattttatt cgtttaaaaa taaaatgtgc
    20881 agcaggttat aattttgcat ttcgctattt ccgcacttct tatttgccgc gcataatccc
    20941 tcgttttaca gatgcccctt taattttggc gaaggatttg tctatggctg ggaatgttca
    21001 ggaaaaacag ctgcgatggt acaacattgc gctgatgtct tttatcactg tctggggttt
    21061 tggcaacgtt gttaataact acgccaacca ggggctggtg gttgtttttt catgggtgtt
    21121 tatctttgcg ctctatttca caccttatgc gctaattgtt ggtcagttag gctcgacctt
    21181 caaagatggg aggggcgggg tcagtacctg gattaaacac acgatgggac ccggactggc
    21241 ttacctcgcc gcgtggacct actgggtagt gcatattccc tatctggcac aaaaacccca
    21301 ggcaatactg attgcgctcg gttgggcgat gaaaggcgac ggttcgttaa tcaaagaata
    21361 ttcagtcgta gcgttacagg ggttaacgct ggtgctgttt atcttcttta tgtgggttgc
    21421 ttcacgcggt atgaaatcgc tgaaaatcgt cggttctgtg gcagggattg cgatgtttgt
    21481 tatgtcgctc ctgtatgtgg cgatggcggt aaccgcgcct gcaattactg aagtgcatat
    21541 tgcgaccaca aacattacct gggaaacgtt cattcctcat atcgacttta cctacattac
    21601 cactatttca atgctggttt tcgcggttgg cggagcagag aagatttctc cttacgttaa
    21661 tcaaacgcgc aacccaggaa aagaatttcc aaaagggatg ttatgcctgg cggtgatggt
    21721 tgcggtttgt gccattctgg gctcgctggc gatggggatg atgtttgatt cgcgtaatat
    21781 cccggatgac ttaatgacca acggtcagta ttacgccttt cagaagctgg gcgagtatta
    21841 cagcatgggt aatactttaa tggtgattta cgccattgcg aataccctgg gacaagtagc
    21901 ggcgctggta ttctcgattg atgccccgct taaagtgcta ttaggtgatg ctgacagcaa
    21961 atatattcca gccagtttat gtcgtaccaa cgcttctggt acgcccgtta atggctattt
    22021 tctgaccctg gtactggtgg cgattctgat tatgctgccg actctcggca ttggtgatat
    22081 gaacaatctc tataaatggc tgttgaacct taattcggta gtgatgccgc tgcgttattt
    22141 atgggtattt gttgcattta ttgcagtcgt tcgcctggcg cagaaatata aaccagaata
    22201 tgtctttatt cgtaataagc ctctggcaat gaccgtcggg atttggtgtt ttgcctttac
    22261 cgcctttgcc tgtttgacgg ggatcttccc gaaaatggaa gccttcactg cagagtggac
    22321 cttccagttg gcgctgaatg ttgcaacgcc gtttgtgctg gtaggattag gactgatttt
    22381 cccgctgctg gcgcgtaaag cgaatagtaa ataattattg tggtcggtga cgctgactct
    22441 ggcagatctg acccgttttt acataatcga gaaatgcgcg caagccgcag gacatatact
    22501 ggcggtttgg ataatagatc tggaagcctg gacgctctgt gctccagtct tccagtacac
    22561 attctaaacg accagtatca aggtactcct tgatctgttc atagagcaga tacccgatac
    22621 cagcacccat caaaacggcc tccagttcag catcaacatc atccagaatg atatttcctg
    22681 cgacagcaat ttccagctcc tttgcaaatt gccagtggaa aggtttgccg ctgggataac
    22741 gaaaaacaac acattgatga ttaagcagat catgaggatg gcggggtttg ccatatcttg
    22801 caaagtactc tggcgttgct gctacacata atttcactgg cgggccgatc gccacgctga
    22861 tcatatcttt ttcgacgatg ccactaagtc gaacgccagc gtcaaagccc tgttggacga
    22921 tatcgacaag agagtcgtca gttgtgagct cgactttaat atcaggatat tcacgtgtga
    22981 aaccaaccag taaaggcatt aaaaatatac gtgcagctac ccgggcggca ttgattttga
    23041 gcgttcctgt cggtgtcaga cgaaaatcat tcatctcatc taacatgatc tgaatttcgt
    23101 cgaaagctgg gcgcaatcgt tcatagagat tagaacccgc ttccgttaac gatacgcttc
    23161 gggttgtccg attgaataag cgaattttaa gacgttgttc cagtgtttta atactatggc
    23221 taatggcgga cgaggataag cctaactcat cgcccgctgc acgaaagctt tgattacggg
    23281 ccacggcaaa gaaagtggca aagtcagaca tattcatccg cattagtgaa aatccttcat
    23341 taactcatcc tgaatcatca cgtttacagg attatgccgc ctgcgtaaag tgcgctccag
    23401 aacttaacgt ggaggtaaaa ttatgcagtc tgaacgtatt tatttggtat gggcccatcc
    23461 tcgtcatgat tcattgaccg cacatattgc tgatgcgatc catcagcggg caatggagcg
    23521 gaaaatacag gtgacggaac tcgatttata tcggcgtaat ttcaacccag tgatgacgcc
    23581 ggaagatgaa ccagactgga agaatatgga taaacgttat tctccagagg ttcatcagct
    23641 ttattcagag ctgcttgaac atgacacgtt agtggtggtt tttcctctct ggtggtacag
    23701 cttcccggca atgctaaaag gatatattga cagagtatgg aataatgggc tggcttatgg
    23761 agatgggcac aaattaccat tcaataaagt tcgttgggtg gcgctggttg gaggagacaa
    23821 agaatcattt gtccagatgg gctgggaaaa aaatataagc gattatttaa aaaatatgtg
    23881 cagttatctt ggtattgaag atgccgatgt cactttcttg tgtaatacag tggtattcga
    23941 tggggaagaa cttcacgcga gctattatca gtcgttatta tctcaggtac gggatatggt
    24001 agatgcacta taagatgtgt taaaaacgct gtagcagaat gaagcgcgga ataaaaaagc
    24061 ggcaactcaa taaagttgcc gctttacggg gaaattagaa cattacctta tgaccgtact
    24121 gctcaagaat gcctttcacg cgttccatgg tctctttctt cggtggttta acaccgtcga
    24181 gtttgtactc ttcacccatt gccacccatt tgtgtttgcc cagctcgtgg taggggagaa
    24241 gctcgatttt ctcaacgttg cccatatcac gggtaaattc accgaggcga tgcgctgaat
    24301 cgtcatcgtc agaccagcct gggacaacaa cgtagcggat ccacaccttc acatttttgt
    24361 tcgccagata tttagcgaac tccagcgtgc ggtggttgga aactccaacc agattttggt
    24421 ggatctcgtc gttcatctgt ttgagatcga gcattaccag gtcggttact tccagcagtt
    24481 catcaatcac cggatcgtaa cgacgaacaa aaccgttggt gtccagacag gtatgaatgc
    24541 cttctttttt gcaggcgcgg aaccagtcac gaacaaactc agcttgcagg attgcttcac
    24601 cgccggatgc ggtaacgccg ccgccggaag cgttcataaa gtggcgatag gtcaccactt
    24661 ccttcatcaa atcttcaacg gtaacttctt taccgccatg cgtgtcccag gtgtcgcggt
    24721 tatgacaata caggcagcgc atcaggcagc cctggaaaaa ggtgataaag cgaatacctg
    24781 ggccgtctac ggttccacag gattcaaagg agtgaatgcg accaataact gacattgcgg
    24841 tgtttctcca gatgtggccc atctgaggcc gtgttggtgc gcagctcgaa ggctacgtcg
    24901 agtctgtttt ggcagtcacc ttaaagtata gatagctgac aaaaaaggct ctcgtgctaa
    24961 aaaaggcccc actttcgtgg agcctttatt gtacgctttt tactgtacga tttcagtcaa
    25021 atctaattac atagattgag tgaaggtacg agtaataacg tcctgctgct gttctttagt
    25081 cagcgagttg aaacgtactg cgtagccaga tacacggatg gtcagctgcg gatatttttc
    25141 cgggttttcc atcgcgtcga gcagcatttc acggttcatc acgttaacgt tcaggtgctg
    25201 accaccttcg atggatgctt cgtggtggaa gtaaccatcc atcagacctg ccaggttggt
    25261 cttacgaact tcgtcgtctt tacccagtgc gttcggaacg atagagaagg tgtaggagat
    25321 accatcttta gcgtaagcaa acggcagttt agcaacggaa gtcagagacg ctacagcacc
    25381 tttctggtca cgaccgtgca tcgggttagc acccggtccg aacggcgcgc cagcacgacg
    25441 accgtctggg gtgttaccag ttttcttacc atacacaacg ttagaagtga tggtcagaac
    25501 agactgagtc gggatagcgt cacggtaggt gtgcagtttc tgaattttct tcatgaaacg
    25561 ttctaccagg tcaacagcca ggtcatctac acgcggatca ttgttaccaa actgcgggta
    25621 ttcgccttcg atttcgaagt cgatagccag accgtcttcg tcacgaatcg gtttaacttt
    25681 cgcatatttg attgcagaca gggagtcagc agcaacggac agaccagcga taccacacgc
    25741 catggtgcgg ataacgtcac ggtcgtgcag cgccatcaga gaggcttcgt agctgtactt
    25801 gtcgtgcatg tagtggatga tgttcagtgc agtgatgtac tgtttagcca gccagtccat
    25861 gaagtgatcc atgcgctcca tcacttcatc gtagttcagg agatcgcctt tgatcggttc
    25921 agacttcgga ccaacctgca ttttcagttt ttcgtcaacg ccgccgttga ttgcgtacag
    25981 catggttttc gccaggtttg cacgcgcacc gaagaactgc atttgtttac caacgatcat
    26041 cgggcttacg cagcaagcga tagcatagtc atcgttgttg aagtccggac gcatcaggtc
    26101 atcgttctca tactgcagag aagaggtgtc gatggacact ttagcggcga atttcttgaa
    26161 gttcagcggc agtttttcag accacagaat ggtcatgttc ggttccggag acggacccat
    26221 ggtgtacagg gtgttcagga aacggaagct gtttttggta accagggtac gaccgtcgag
    26281 gcccatacca ccgatagatt cggttgccca gatcgggtcg ccagagaaca gttcatcgta
    26341 ttccggagta cgcaggaagc gaaccatacg cagtttcatg accaggtggt caaccatttc
    26401 ctgcgcttct tgttcggtga tcttgccagc tttcaggtca cgttcgatgt acacatccag
    26461 gaaggtggag gtacgaccga aggacattgc agcaccgttc tgagacttaa cagcagccag
    26521 gtagccgaag taagtccact ggatagcttc ctgagcgttg gtagccggac cagagatgtc
    26581 gtagccgtat ttcgcagcca tttctttcat ctgacccaga gcgcggtgct gttcagcgat
    26641 ttcttcgcgc agacggatag tctgttccag gtttacgccg ttttccagat cagtctgcag
    26701 agaggtgaac tgagcgtatt tgtctttcat caggtagtcg ataccgtaca gcgcaacgcg
    26761 acggtagtca ccgatgatac ggccacggcc ataagcatct ggcagaccgg tcagaacacc
    26821 ggatttacgg caacgcagga tgtccggagt gtaaacgtcg aacacgccct ggttgtgagt
    26881 tttacggtat tcagtgaaga tttttttgat catcgggtcc agttcgcggt tgtacgcttt
    26941 gcaggaacct tcgatcattt tgataccacc gaacgggata agagcacgtt tcagcggagc
    27001 ttcagtctgc agaccaacaa ctttttccaa cgctttgttg atgtaaccag cgtcgtgaga
    27061 ggtgatggta gaagcaacag cggtgtcaaa gtcaactggc gcgtgagtgc ggttttccag
    27121 tttaacgcct tccattactt tgtcccacag ggtggtggtc gcttcagtag cgccagccag
    27181 gaaggactcg tcaccctcgt acggagtgta gtttttctga atgaagtcac ggacgtttac
    27241 ttcattctgc cagtcacctt tggtaaaacc ttcccaggct gtggctaact tttcattaag
    27301 ctcggacatg taacacctac cttcttaagt ggatttttta tttactgcgt acttcgacaa
    27361 ccattaatgg tgatcgtttt cacgcaggta aatgacccag tatgtcaacc caaccaacaa
    27421 accaccgccg ataatattac cgatcgtaac cggaatcagg ttatcagtga tgaagttcat
    27481 cacggtcagg tgagaaaaat tttccggtgc agaaccgaca gcggtccaga attccggaga
    27541 tgcgaagtcg cggattacaa tacccatcgg gatcataaac atgtttgcga tactgtgctc
    27601 aaaaccgctg gcaacaaaca tcgcgaccgg cagcaccata atgaacgctt tgtccatcag
    27661 gctacggcca gaataactca tccatactgc cagacatacc atcaggtttg ccaggatacc
    27721 aaggcagacg gcctcaataa aagtatggtg cactttgtgg tcggcggttt gtatgacgtt
    27781 tagtccccat tgaccatttg cggtcatata ctcgccggaa agccacatta aaagtacaaa
    27841 cagcagtgcg ccgaccaggt tgccaaaata gacatttagc cagtttttcg ccaactgacc
    27901 ccaggtgatg cgcccactcg ccttagcaac aacaatcaac actgtggaag taaagagatc
    27961 ggctccgcag acaacacaaa gaatcagccc cagagagaag caaatgccgc caaccagttt
    28021 tgccatgccg aagggcattg tgcctgtgcc agtggttgct gtgatataga agacgaatgc
    28081 gattgagatg aaaacaccgg cggtaatcgc cagatagaaa gtcttaagcg gatgtttcgt
    28141 tgctttatag acacccgcct cttcggccac tttggccatt gcagcaggaa gtaaaagatc
    28201 aaaagggttg tcagctttca cactaactct ctctttatta agtcggcgac gagatactaa
    28261 caaagcatta tagatgagaa attgatatag atcatatctc gcctggctta taggcccgta
    28321 actc
//
LOCUS       DAFNBJ010000054        28161 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000054 DAFNBJ010000000
VERSION     DAFNBJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.054"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="L2X49_002158"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="L2X49_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBN4797240.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="L2X49_002159"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="L2X49_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBN4797241.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="L2X49_002160"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="L2X49_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBN4797242.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="L2X49_002161"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="L2X49_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBN4797243.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="L2X49_002162"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="L2X49_002162"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBN4797244.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="L2X49_002163"
     CDS             complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="L2X49_002163"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBN4797245.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="L2X49_002164"
     CDS             complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="L2X49_002164"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBN4797246.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4523..5245)
                     /locus_tag="L2X49_002165"
     CDS             complement(4523..5245)
                     /locus_tag="L2X49_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN4797247.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="L2X49_002166"
     CDS             complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="L2X49_002166"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBN4797248.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6445..6663
                     /locus_tag="L2X49_002167"
     CDS             6445..6663
                     /locus_tag="L2X49_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBN4797249.1"
                     /translation="MQDLSLETRLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="L2X49_002168"
     CDS             complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="L2X49_002168"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBN4797250.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7397..7597)
                     /locus_tag="L2X49_002169"
     CDS             complement(7397..7597)
                     /locus_tag="L2X49_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBN4797251.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="L2X49_002170"
     CDS             complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="L2X49_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBN4797252.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="L2X49_002171"
     CDS             complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="L2X49_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBN4797253.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10094..12007
                     /locus_tag="L2X49_002172"
     CDS             10094..12007
                     /locus_tag="L2X49_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN4797254.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12007..13029
                     /locus_tag="L2X49_002173"
     CDS             12007..13029
                     /locus_tag="L2X49_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBN4797255.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13023..13241
                     /locus_tag="L2X49_002174"
     CDS             13023..13241
                     /locus_tag="L2X49_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBN4797256.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13295..14164
                     /locus_tag="L2X49_002175"
     CDS             13295..14164
                     /locus_tag="L2X49_002175"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBN4797257.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14219..14623)
                     /locus_tag="L2X49_002176"
     CDS             complement(14219..14623)
                     /locus_tag="L2X49_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBN4797258.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14925..15557
                     /gene="crp"
                     /locus_tag="L2X49_002177"
     CDS             14925..15557
                     /gene="crp"
                     /locus_tag="L2X49_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBN4797259.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15608..17698
                     /locus_tag="L2X49_002178"
     CDS             15608..17698
                     /locus_tag="L2X49_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBN4797260.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFAACSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17765..18985)
                     /gene="argD/dapC"
                     /locus_tag="L2X49_002179"
     CDS             complement(17765..18985)
                     /gene="argD/dapC"
                     /locus_tag="L2X49_002179"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBN4797261.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19071..19634)
                     /gene="pabA"
                     /locus_tag="L2X49_002180"
     CDS             complement(19071..19634)
                     /gene="pabA"
                     /locus_tag="L2X49_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBN4797262.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19666..20268)
                     /locus_tag="L2X49_002181"
     CDS             complement(19666..20268)
                     /locus_tag="L2X49_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBN4797263.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20258..20425)
                     /locus_tag="L2X49_002182"
     CDS             complement(20258..20425)
                     /locus_tag="L2X49_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBN4797264.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20530..21102)
                     /gene="ppiA"
                     /locus_tag="L2X49_002183"
                     /gene_synonym="rotA"
     CDS             complement(20530..21102)
                     /gene="ppiA"
                     /locus_tag="L2X49_002183"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBN4797265.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21373..22554
                     /gene="tsgA"
                     /locus_tag="L2X49_002184"
     CDS             21373..22554
                     /gene="tsgA"
                     /locus_tag="L2X49_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBN4797266.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22816..25359
                     /gene="nirB"
                     /locus_tag="L2X49_002185"
     CDS             22816..25359
                     /gene="nirB"
                     /locus_tag="L2X49_002185"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBN4797267.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25356..25682
                     /gene="nirD"
                     /locus_tag="L2X49_002186"
     CDS             25356..25682
                     /gene="nirD"
                     /locus_tag="L2X49_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBN4797268.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25809..26615
                     /gene="nirC"
                     /locus_tag="L2X49_002187"
     CDS             25809..26615
                     /gene="nirC"
                     /locus_tag="L2X49_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBN4797269.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26634..28007
                     /gene="cobA"
                     /locus_tag="L2X49_002188"
     CDS             26634..28007
                     /gene="cobA"
                     /locus_tag="L2X49_002188"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBN4797270.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6808 a         7562 c         7125 g         6666 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcac gacctttggt
      301 cagagaacgc agctgagttg cgtatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttccgg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcagac cgatagcagc agcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtaaacac ggaagaaggt
     1321 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgttcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca tgcggtccat tttgttaacg aacgcaatgc gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagcgcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccgccac ggatcaggat
     3121 cacggagtgc tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggagtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atgggaatga tgtcgttcga aatttgacca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agggcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt accgtcaact
     3661 gcggcagtta cgccatcttg caataatagc agttcgtctc cttcactgag cagacgcagc
     3721 agcgcagcaa aatccgtcag ccagggtgaa cgatgtaacg tgtgcagcat aaacgcctca
     3781 aaacctcaaa ataacatcgt agttggcgag ttcgcggcgt aaggcatctg cttcgagcgg
     3841 cgtggcttcg acaacaaagg gtgtctgcgg atctaacccg cgttcgcgca gtgaagccgc
     3901 acaaacccag cactgttcaa tgtcgtacag acccaacaat ttaaaagtgg caatgtaatc
     3961 acgcgccagc actgcatcgg gcttttgtcc tgacagcagc tgaaaaacgc catcagcaat
     4021 aaagaagacg gccagatcgt cagttaatgc ggaagttgcc agtaaagcat ctaaaccttc
     4081 ccgaccagca gctgtaccat gaggtgcagt agaaaaaaca aacgcaattc gtttcatcag
     4141 aactgtacca ccctgtcaca ggtcagcgag gcttccgcca gcgcgccaag tccgcttaag
     4201 gtaaatcccg gctgaaggtt tgacgaagcc agccccagcc ttcctgcttc agtttcatcg
     4261 acgataccgc ggcgtaacgc tgccgctacg cagatattca gcgccacacc atgttgcgca
     4321 ttcaactgtt gccagccgcg cacaaggtca aattcgtcac ttgccggtga ggtcaattgg
     4381 ttagcgttat agaccccttc ccgatagaaa aagacgctgc ttaactcatg gccttctact
     4441 atcagcgcct gcgcaaactg aaaagcacta ctcgcctgtt gcgtaccgta tgctggcccg
     4501 gtcaccacga tggcaaaacg cattacttat cttgcccctg gaaatcaccg cttttgaact
     4561 ggcggatgta gagatagaca gtgtgtttgg agatgttcag gcggtcagca acctggttga
     4621 tcgcgtcttt aatatcgaag atccctttct cgtagagatt cagcacgatc tgacgatttt
     4681 tggcgttatt agaaacattg cgatcggcat ttacttcttc gatggtgaac tccagcgttt
     4741 gggtaaccag atcttcaaca gaagaggcaa agttgacgct tgaaccgaca tccggggttt
     4801 ctggcggcac aaaagtgctc ataatctgcg agaagggaac atcaagattc atattgatgc
     4861 acagcagacc aattacacgc tgttcgcggt tacgaatcgc gatagtcagg gacttcatta
     4921 atacgccgct tttggcgcga gtaaagtagc atttagaaac gctgctatcc gctcccgtca
     4981 tatcgtgcag catacgtagc gccaggtcag taattggcga accaatcttc cggcctgtat
     5041 gttcaccgtt agcaatgcga atggctgaac attttagatc ctgcaaagag tgcaaaacga
     5101 tttcacagtg ggagccaata agcatcgcta acccgtccac caccgcttcg taggatttca
     5161 gaatatcaaa atcggtctgg tcgaaaggac gttgatccag taaatccaac tcactggttt
     5221 cgttggttaa aagcgacctg gacatgaaaa aaaacactcc ttttcaggag cctgtcgtta
     5281 acttttcagg gcaggctcat taatgatgcg ggtaactaaa ttaatacagc ggaggttccg
     5341 ctttccagca ctaattatat ccggcctgta ataaaaaaac cgccgcctgg tcaggcggcg
     5401 gttcttaatg cttatttttt agcagaatct gcggctttcg catcagcttc cggctttgca
     5461 tcagccttcg gcgctggttt cacgtccaac agctctacgt caaacaccag ggtagaattc
     5521 ggtgggatcc ccggaacacc cgctttgccg taagccagtt ctggtggaat aaccagtttg
     5581 atcttaccgc ctttcttgat gttcttcaga ccttctgtcc aacccgggat aacaccgtcc
     5641 agacggaaag agagcggttc accacgagtg taagagttgt cgaactcttt accgtcgatc
     5701 agagtacctt tgtagttcac gacaacagta tcgctgtctt tcggtgcttc gcctttaccg
     5761 gcttctacta cctgataaac cagaccagtt gaagaggttt tcacaccttt ctctttggca
     5821 aatttctcgc ggtactcttt accttttgct tcgttatcag ccgcgtcttt ttccatcttc
     5881 gcctgagcag aagacttcac gcgagcttcg aatgcttgca gagtctgttc gatctcttgg
     5941 tcggagagtt tgctcttatc agcaaatgca tcctgaacac cagcgatcag ctgatcttta
     6001 tccagtttga tgcccagttt ttcttgttct tttagagagt tttccatgta acgacccagc
     6061 gaggcaccca gtgcataagc tgatttctga tcgtcatttt tgaacgctgc tttgctgtca
     6121 gcagttgtag caggttttgc agcttcagca gcaaaagtga ttggtgcatg cagggcaacg
     6181 gccattgtgg tcgccagcag cgttacttta aacagtgatt tcatccatat ctccaggatc
     6241 ggggcatctc accccagggt taactattat cagaagggta ctataaagcg ttgtcgaaca
     6301 aatctacata cagacacgcc ctattatcat ctattttcag actctttttg tttaaattag
     6361 tttcgatgac cgcgaaatga gtgctgtctc gggcagcaaa gttaagtaga atccgcggcg
     6421 accattcgac aaaagaggtg aatcatgcag gatttatcat tggaaacacg cctggcagag
     6481 ctggagagcc gactggcttt tcaggaaatc accattgaag aactgaacgt cacggtgacc
     6541 gctcatgaaa tggagatggc gaaactgcgc gatcatctgc gtctgctgac cgagaagtta
     6601 aaagccagcc agccgtcgaa catcgcgtcg caggctgaag aaacgccacc gccacattat
     6661 tgaggcgtaa aaaaagcggg gattccccgc ttttttgtca ctttttcggt attagtggca
     6721 accgcaaccg ccgttgcctt taccgccaca gcagccttcg ccaccgtgtt catgaccgtg
     6781 atcgtggcca tgaccgccgc agcaaccgtc gtggtcgtga tcgtggtggt gatcgtgcgc
     6841 gccgtgaacg tgaccatgag ccagttcttc ttcagtcgct tcgcgaatcg ccacaacttc
     6901 aacgttgaat ttcaggttct gaccggccag catgtggtta ccatcaacca cgacgtgatc
     6961 gtcttcaacc gcagtgattt caaccggtac cggaccctgg tcggtttcag ccaggaaacg
     7021 catacctacc tgcagttcat caacgcccat aaatacgtct ttaggaacac gttgcaccag
     7081 gttttcgtcg tactgaccgt aagcgtcgtt cgcgccaaca gcgacatcaa atttgtcgcc
     7141 aacttcatga ccttccagcg ccgtttccag gccagagatc agggaaccgt gaccatgcag
     7201 gtagtccagc ggcgcactca ccggagactc atcaaccaac acaccgtctt ctgtacgtac
     7261 ctgataggcc aggctgacca ccaggtcttt tgctactttc atgatatctc ctgagcatgg
     7321 gaagaatagt ggcgcagatt gtagcggaat tctgcagccg tgtactcact agcttaaaaa
     7381 aactcggcgc atatcgctag tccggatgaa aaatcccgat cacttgctca tctttgcgaa
     7441 cgtgatcgcg ggcttctttg tctgcttctc gcatctgatg tccgcactta acacattcaa
     7501 caatatcaat attattttcg cgccacatcg ccattgaatc ctgcgcctga caggccgggc
     7561 attttgcgcc cgcaataaaa cgttttcgga ttgccatctt taccctctac tcaaattcat
     7621 cccagccgtc cagctggcgt cgttcttgtt gcatttcacg ctggaaaatc tcttccagtt
     7681 cgcgtcgggc ttccctggcg cgagaaattt gtacggtatc ggcatgcatt gggatgagct
     7741 ctcgcagcat ccgcatatcc aggcggcgaa aatgcagttg cgctcgctgt gcctgatgcg
     7801 gatgcatgcc aagcgtgacc agcgtcttgc gccccagctc taacgcactg gagaatgttt
     7861 cacgggaaaa ctgcgtcacc cctgcctgta ataactcatg cgcttccaca cgtccgcgcg
     7921 ctcgcgcaag aatatgcaaa tgcggaaagt gctgctggca tatttccacc agcttcatgg
     7981 tgtcttccgg ctcgttacag gtaatgacga tagactcagc ggcctctgca cccgcagaac
     8041 gtaaaagatc gacctgcgtg gcgtcgccgt aataaacttt gtagccgtat ttacgcatca
     8101 ggttaacggc gctgatatcc cgctccagca cggtaatgcg cattttattt gccatcagca
     8161 aacgaccaat cacctgacca aaacgcccga agcccacgac aatgacctgg ggtttatcat
     8221 cgttgaccca cggtttttcg tcttcttctt ccggtccgtt aaactggcgg gatagccatt
     8281 tatccaccag cttcatcagc aacggcgtgg tcatcatgga aagcgtcacc gtcaccagca
     8341 acaacgccat ctggtcgccc tggaataagc gttgtgaaga agcggtagaa aagaggacaa
     8401 aggcaaactc accaccctga ctcaacacgc cagcaaactg catccgctct gagctacgca
     8461 cgccatacaa tcgcgccagc agatacagca cgagaatttt caccgccacc agcacaacca
     8521 cgcttatcac tacccacaac agatgggtat aaagcacccc gaggttgagt gacatgccga
     8581 cagagataaa gaacaaaccg agcagcaagc ctttgaaggg atcgatagcc gtttccagtt
     8641 catggcgata ttcactttcc gccagcagca cgcccgcaat aaatgtaccg agcgccatcg
     8701 acagccccag cgcatccata aacaatgcgg aacccaacac cagcagcagc gtcgcggcgg
     8761 tgaacacttc ccgcacgcca gaagctgcaa taaagcggaa taccggacgc agtaaatagc
     8821 gcccaccaat cagcatgccg acaaacgcca gcaccttcat gccgatcttc atccagtcga
     8881 aatgttcgtc tgccgacccc gccaacaacg gcactaacgc cagtgctggg attactgcca
     8941 gatcctgaaa cagcagaacc gaaaacccga gctggccgga ttcgctgcga ttcatccctt
     9001 tctcacgcat caattgcaat gccattgcag ttgaagacat cgcaaggcca atgccaccga
     9061 ccaccgccgc ctgccaggcg aaatccgtca gcatcaataa tcccgccagc aacgccgcgc
     9121 ttaacagcac ctgtgccgcg cctacgccaa aaatcgaacg ccgcagttgc caaagtttgg
     9181 agggattcaa ctcaaggccg atgataaaca tcaggaatac cacgccgagt tccgaaaagt
     9241 ggaggatctc atcgacgtcg ctaataaacc ccagccccca cgggccaatt gcaatccctg
     9301 ccagcaaata tcccaacaca gcgccaatac ccagccgcga tgccagcggc accgcagcca
     9361 ccgccgcgaa gagaaacagc actcctgcga gtaaaaaatc ggaaccttcc atcagcggcc
     9421 tcctggagac agcggatttg ccagccagtc accgtaggct ctggcgtggc tcgccagctc
     9481 ctgtgcgctt tgccgtctcg cccagtaaat aatgatggga cttaaccaat gcatccggca
     9541 catgcccgcc gccagttcaa aggggcgcag cacatcgctc atcgggtagc gattcagcgc
     9601 gtcataacgg taagcacttt ctggctcgcc ggtggtaatc acgctacgcc agtactttcc
     9661 cgccagttgg tttcctcccg gcccgctggc aaaaccacga cttaataccc gatccagcca
     9721 ctctttcagt agcgccgggc agctataggt ataaagagga tgctgaaaga caatcacctc
     9781 gtgctcgcgc agtaatgcct gctcacgggg gatatcaata aaaaaatcgg gatagtgcgc
     9841 gtaaaggtcg tgcacggtaa cattgctgag ctgcgtggcc ggtttaagca gtacccggtt
     9901 tgccaccgag tcctgagatt ccggatgggc atacagcagc aaaactttcg ctggctgaga
     9961 catcatcccc ctcccggtat ggtttttgtg tatagtcgct gttttgggct accattgcgc
    10021 ccggtgcggc agctcgccca tacattacat tatcataatg ataagttaac atagtctgaa
    10081 catacggcac cttatgattg ttttctcctc gttacaaatt cgtcgcggcg tgcgcgtcct
    10141 gctggataat gccaccgcca ccatcaaccc agggcagaaa gtcggcctgg tgggtaaaaa
    10201 cggctgtggt aaatctaccc tgctggcatt gctgaaaaat gaaatcagcg ccgacggcgg
    10261 cagctacacc tttccgggaa gctggcaact ggcgtgggtg aatcaggaaa cgccggcgtt
    10321 accgcaagcg gcgctggaat atgtcattga cggcgaccgt gaatatcgtc aactagaagc
    10381 gcagctacac gacgccaacg aacgtaacga cgggcacgcc attgcgacca ttcatggcaa
    10441 gctggatgct attgacgcat ggagtattcg ctcccgtgct gccagcctgc tgcacggcct
    10501 cggtttcagc aatgaacaac tggagcgccc ggtaagtgat ttttccgggg gctggcgtat
    10561 gcgtcttaac cttgcccagg cgctgatttg ccgttcagac ttgctgctgc tcgacgaacc
    10621 gactaaccac ctcgatctcg atgccgttat ctggctggaa aaatggttga agagctatca
    10681 gggcacgctg atcctgatct ctcacgaccg cgacttcctc gatccgatcg ttgataaaat
    10741 tattcatatc gaacaacaaa gcatgttcga gtacaccggt aactacagtt cgtttgaagt
    10801 acagcgcgcc acccgtctgg cgcagcaaca agcgatgtat gaaagccagc aggaacgcgt
    10861 agcgcatctg caaagttata tcgaccgttt ccgtgccaaa gccaccaaag cgaagcaggc
    10921 ccagagccgc attaagatgc tcgagcgtat ggagctgatt gcccccgcgc acgtcgacaa
    10981 cccgttccgc tttagcttcc gcgcgccgga aagcctgcca aatccgttac tgaagatgga
    11041 aaaagtcagc gcgggctatg gcgatcgcat tattctcgac tcgattaaac tgaacctggt
    11101 gcccggctcg cgcattggtc tgttaggccg caacggcgcg ggtaaatcga cattaatcaa
    11161 actgttagcc ggtgaacttg cgccagtcag cggtgaaatt ggtctggcga aagggatcaa
    11221 gctcggctac ttcgcccagc atcaacttga atacctgcgc gccgacgaat cacctattca
    11281 acatctggca cgtttagcgc cgcaggagct ggaacaaaaa ctgcgtgact acctcggcgg
    11341 ctttggtttc cagggcgata aagtaaccga agaaacgcgc cgcttctcag gtggggaaaa
    11401 agcccgcctg gtgctggcat taattgtctg gcagcgtccg aatctgctgc tgctcgacga
    11461 accgactaac caccttgacc tcgacatgcg tcaggcactc accgaagcat taatcgagtt
    11521 cgaaggcgcg ctggttgtcg tttcgcacga ccgtcatttg ctgcgttcca ccactgacga
    11581 tctctacctg gttcacgatc gtaaagtcga accgttcgac ggcgatctgg aagattatca
    11641 acagtggttg agcgacgtac aaaagcagga aaaccaggcc gacgaagcgc caaaagagaa
    11701 cgcgaacagc gcccaggcac gtaaagatca gaagcgtcgg gaagcggagt tgcgtgcgca
    11761 aacccagcca ctgcgtaaag agattgcccg tctggaaaaa gagatggaga agctgaacgc
    11821 gcaactggcg caggcagaag agaaactcgg cgacagcgaa ctgtatgacc agagccgtaa
    11881 agcggagttg accgcctgcc tgcaacagca agccagcgcc aaatccggcc tggaagagtg
    11941 cgaaatggca tggctggaag cccaggagca gcttgagcag atgttgctgg aaggccaaag
    12001 caactgatgg cgcagataac gacgaccgat gccaatgaat tcagcagcag tgctgaattc
    12061 acccctatgc gcggctttag caattgtcat ctgcaaacca tgctgccgcg tctgtttcgt
    12121 cgcaaggtga aattcacccc gtactggcag cggctggagt tgcccgacgg cgattttgtc
    12181 gatctcgcgt ggagtgaagc ccctgcacag gctcggcata aaccgcgttt agtggtattt
    12241 cacgggctgg aaggcagtct caatagccct tacgcccacg gtctggttga ggcggcgcaa
    12301 aagcgcggct ggctgggcgt ggtgatgcat tttcgcggat gcagcggtga accaaaccgt
    12361 atgcaccgca tttaccattc gggcgaaacc gaagacgcca gttggttttt acgctggctg
    12421 caacgcgaat ttggtcatgc gccaacggct gccgtcggct attcgctcgg cggtaatatg
    12481 ctggcctgtt tgctggcaaa ggaaggcaat gatctcccgg ttgatgcggc ggtgattgtc
    12541 tctgcgccgt ttatgctgga agcctgtagc tatcatatgg aaaagggctt ttcccgcgtt
    12601 tatcagcgtt acttgctgaa cctgttaaaa gccaatgccg cgcgcaagct ggcagcctac
    12661 cccggaacgc tgccgattaa tctcgcgcag ttaaaatcgg tacgtcgcat ccgtgaattt
    12721 gacgatctaa tcaccgccag aattcacggc tacgccgacg ctatcgacta ttatcgtcag
    12781 tgtagcgcca tgccgatgct gaaccggatc gccaaaccga cgctgattat tcacgccaaa
    12841 gacgatccgt ttatggatca tcaggtgatc ccgaaaccgg aaagtctccc ctcgcaggtg
    12901 gagtatcaac tgactgaaca tggcggtcat gttggcttta ttggcggtac attacttcat
    12961 ccgcaaatgt ggctggagtc acgcattcct gactggttaa caacgtatct ggaggcgaaa
    13021 tcatgttgat tccgtggcaa gacctctccc ccgaaacgct ggaaaattta attgaaagct
    13081 ttgtgttacg tgaaggcacc gattatggtg aacatgagcg tacacttgaa cagaaagtcg
    13141 ccgacgtcaa acgccagcta cagtgcggag aagcggtgct ggtatggtcg gagctgcacg
    13201 aaacggtcaa tattatgccg cgcagtcagt ttcgcgaata acctgcaacc gtcggcggac
    13261 gagataataa tgatagctaa gtcatggagt tactatgtct gccaaacatc cggtcattgc
    13321 ggtaacagga tccagcggcg cggggaccac caccaccagc ctcgcgtttc gtaaaatatt
    13381 cgcgcagtta aatctgcatg cagctgaggt ggaaggcgac agttttcacc gttacacccg
    13441 cccggaaatg gacatggcga tccgcaaagc gcgcgacgcc gggcggcata tcagctactt
    13501 cggccccgaa gcgaacgact tcggcctgct ggaacaaacc ttcattgaat acggtcagag
    13561 cggcaaaggg aaatctcgca aatatctgca tacctacgac gaagccgtac cctggaatca
    13621 ggtaccggga acattcaccc cctggcaacc tttaccagaa cccactgatg tactgtttta
    13681 tgaaggttta cacggcggcg tagtcacgcc acagcataac gttgcgcagc atgtggactt
    13741 actggtcggc gtagtgccta tcgttaacct tgagtggatt caaaaactga tccgcgacac
    13801 cagcgagcgc gggcactcac gagaagcagt gatggactcg gtagtgcgtt caatggaaga
    13861 ctatatcaac tacatcacac cgcagttttc ccgcacccat cttaacttcc agcgcgttcc
    13921 caccgtcgac acttcaaacc cgttcgcggc aaaaggtatc ccgtcgctgg atgaaagctt
    13981 tgtggtgatc cattttcgta atctggaagg aatcgatttc ccctggctgc tggcgatgtt
    14041 gcaaggctca ttcatttccc acatcaatac gttagtggtg cccggcggca aaatgggtct
    14101 ggcaatggaa ttaattatgc tgccgctggt gcaacgattg atggaaggaa agaaaatcga
    14161 gtaactctgc tattacgccg gataaaatac tatccggctt cacaacggga tagttaaatc
    14221 acgcggcaac cacttcatac gagtgagtaa tattcaccgc tttttccagc atcaacgcca
    14281 ctgaacaata tttctcagca gagagatcaa ccgcacgcgc aaccgctgcg tctttcaggt
    14341 cgcgaccggt gacgataaaa tgcagattaa tgtgcgtaaa caggcgtggt gcctcttcgc
    14401 ggcgttcaga ggtcaatttt acttcacaat cgaccacatc ctgacgccct ttttgcagga
    14461 tcgaaaccac atcgatggca ctgcaaccac ccgccgccat caacaccatt tccatcggac
    14521 ttggtgcttt atcgcctgag ttgccgtcca ttaaaatctg atgaccagag gcggattcgc
    14581 ccagaaaagt taacccttcg acccacttca ctcgcgcttg catttttgct actccactgc
    14641 gtcaattttc ctgacagagt acgcgtacta accaaatcgc gcaacggaag gcgacctggg
    14701 tcatgctgaa gcgagacacc aggagacaca aagcgaaagc tatgctaaaa cagtcaggat
    14761 gctacagtaa tacattgatg tactgcatgt atgcaaagga cgtcacatta ccgtgcagta
    14821 cagttgatag ccccttccca ggtagcggga agcatatttc ggcaatccag agacagcggc
    14881 gttatctggc tctggagaaa gcttataaca gaggataacc gcgcatggtg cttggcaaac
    14941 cgcaaacaga cccgactctc gaatggttct tgtctcattg ccacattcat aagtacccat
    15001 ccaagagcac gcttattcac cagggtgaaa aagcggaaac gctgtactac atcgttaaag
    15061 gctctgtggc agtgctgatc aaagacgaag agggtaaaga aatgatcctc tcctatctga
    15121 atcagggtga ttttattggc gaactgggcc tgtttgaaga gggccaggaa cgtagcgcat
    15181 gggtacgtgc gaaaaccgcc tgtgaagtgg ctgaaatttc gtacaaaaaa tttcgccaat
    15241 tgattcaggt aaacccggac attctgatgc gtctgtctgc acagatggcg cgtcgtctgc
    15301 aagtcacttc agagaaagtg ggcaacctgg cgttcctcga cgtgacgggc cgcattgcac
    15361 agactctgct gaacctggca aaacaaccag atgctatgac tcacccggac ggtatgcaaa
    15421 tcaaaattac ccgtcaggaa atcggtcaga ttgtcggctg ttctcgtgaa accgtgggac
    15481 gcattctgaa gatgctggaa gatcagaacc tgatctccgc acacggtaaa accatcgtcg
    15541 tttacggcac tcgttaatcc cgtcggagtg gcgcgttact gagtagcgcg ccattttgtt
    15601 tcccccgatg tggcgcagac tgatttatca ccccgatatt aactatgcac tgcggcaaac
    15661 gctggtgctc tgtttgcccg tggccgttgg gttaatgctt ggcgaattac gattcggtct
    15721 gctcttctcc ctcgttcctg cctgttgcaa tattgcgggc cttgatacgc ctcataaacg
    15781 ttttttcaaa cgcttaatca ttggtgcgtc gctgtttgca gcctgtagct tgctgacaca
    15841 gctgctactg gcaaaagatg ttcccctgcc ctttttgctg accggattaa cgctggtact
    15901 tggcgtcact gctgagttgg ggccattgca cgcaaaattg cttcccgcat cgctgctggc
    15961 agccattttt accctcagtc tggcgggata catgccggtc tgggaaccgt tgctcatcta
    16021 tgcgttgggc actctctggt acggattgtt taactggttt tggttctgga tctggcgcga
    16081 acaaccgctg cgcgagtcac taagtctgct gtaccgtgaa ctagcagatt attgtgaagc
    16141 caaatacagc ctgcttaccc agcacaccga ccctgaaaaa gcgctgccgc cgctgctggt
    16201 gcgccagcaa aaagcggtcg atctaattac ccagtgctat cagcaaatgc atatgctttc
    16261 cgcgcaaaat aatactgact acaagcggat gctgcgtatt ttccaggagg cgctggattt
    16321 acaggaacat atttcggtca gtttgcatca gccggaagag gtgcaaaagc tggtcgagcg
    16381 tagccatgcg gaagaagtta tccgctggaa tgcgcaaacc gtcgccgctc gcctgcgcgt
    16441 gctggctgat gacattcttt accatcgcct gccaacgcgt tttacgatgg aaaagcaaat
    16501 tggcgcactg gaaaaaatcg cccgccagca tccggataat ccggttgggc aattctgcta
    16561 ctggcatttc agccgcatcg cccgcgtgct gcgcacccaa aaaccgctct atgcccgtga
    16621 cttactggcc gataaacagc ggcgaatgcc gttacttccg gcgctgaaaa gttatctgtc
    16681 actaaagtct ccggcgctac gcaatgccgg acgactcagt gtgatgttaa gcgttgccag
    16741 cctgatgggc accgcgctgc atctgccgaa gtcgtactgg atcctgatga cggtattgct
    16801 ggtgacacaa aatggctatg gcgcaacccg tctgaggatt gtgaatcgct ccgtgggaac
    16861 cgtggtcggg ttaatcattg cgggcgtggc gctgcacttt aaaattcccg aaggttacac
    16921 cctgacgttg atgctgatta ccaccctcgc cagctacctg atattgcgca aaaactacgg
    16981 ctgggcaacg gtcggtttta ctattaccgc agtgtatacc ctgcaactgt tgtggctgaa
    17041 cggcgagcaa tacatccttc cgcgtcttat cgataccatt attggttgtt taattgcttt
    17101 cggcggtact gtctggctgt ggccgcagtg gcagagcggg ttattgcgta aaaacgccca
    17161 tgacgcttta gaagcctatc aggaagcgat tcgcttgatt cttagcgagg atccgcaacc
    17221 tacgccactg gcctggcagc gaatgcgggt aaatcaggca cataacactc tgtataactc
    17281 attgaatcag gcgatgcagg aaccggcgtt taacagccat tatctggcag atatgaaact
    17341 gtgggtaacg cacagccagt ttattgttga gcatattaat gccatgacca cgctggcgcg
    17401 ggaacaccgg gcattgccac ctgaactggc acaagagtat ttacagtctt gtgaaatcgc
    17461 cattcagcgt tgtcagcagc gactggaata tgacgaaccg gggagttctg gcgatgccaa
    17521 tatcatggat tcgccggaga tgcagccgca cgaaggcgcg gcaggtacgc tggagcagca
    17581 tttacagcgg gttattggtc atctgaacac catgcacacc atttcgtcga tggcatggcg
    17641 tcagcgaccg catcacggga tttggctgag tcgcaagttg cgggattcga aggcgtaatg
    17701 caggctaaac catcggataa ggcattcacg ccgcatccga catcttttgc ctgatgcttc
    17761 gtatttacgc cccaaccacc ttcgccaccg cgtgggcgaa acgttgcatc ccttcatcga
    17821 tatccgcatc ttccaccacc agcgacggtg caaaacgcat cacatccggt ccggcattca
    17881 gcaccattac gccagcccct gcgcccgcat acaggaaatc acgcgcccga cctttgaact
    17941 gtggcttaag ctccgcgcca atcaacagcc ccataccgcg aatatcgcta aacacatcgt
    18001 actgctgatc gatcttctgc aaatggtcaa caaaacgctg gcgtttcgcc tgaatgcctt
    18061 ccagcacttc aggggtattg atgatatcaa acgccgcgcc cgctactgca caggccagag
    18121 gattaccgcc gtaggtggaa ccgtgagaac caggatgaaa cgcagaagca atttccgccg
    18181 tggtcagcat ggcgctaatc gggaagccgc cgcctaacgc tttcgcagag gtcagaatat
    18241 ccggcgtcac gccgtagtgc atgtaagcaa acaaatcgcc ggtccgcccc atcccgcact
    18301 gcacttcatc aaacaccaat aatgcctgat gttgatcgca cagctcgcgc aagccctgca
    18361 aaaactctgg cgtcgcagcc gtcacgccgc cctcgccctg gatcggctca accaccaccg
    18421 cacaggtgtg atcatccatc actgctttca ctgcgtggag atcgttaaag ggaacgtgga
    18481 tgatgtctgc cggtttcggc ccaaagccgt cggaatattt tggctgccca cccaccgaaa
    18541 cggtaaacag cgagcgacca tgaaaagcgt tatggaaggc aataattttg gttttgaacg
    18601 ggctatgacg cacacaggcg taatgacgtg ccagtttaaa ggcggtttcg ttagcttccg
    18661 tgccggagtt cataaacacc acgcgttcgg caaacgttgc ctcaatcagt ttacgcccaa
    18721 gacgcagcgc cggttcattg gtgaaaacgt tactgatatg ccacagagtt tcgccctggg
    18781 tttttaacgc gttcaccagc gcaggatggc aatggcccaa cgccgtaact gcaatgccac
    18841 ccgcgaaatc gacatactcc ttgccttgct gatcccagat tcggctgccc tgacctttta
    18901 ccggaataaa ctctgccgga gcataaatcg gcaggatcac ttcatcgaaa gtcgcgcgtg
    18961 taattgctgt ttgttcaatt gccatctcat aatcaccctg ttacgcataa ataaatgtga
    19021 aattataatc acaaaatatg cataaaaaat cactaaatgg caatcagaaa tcagcgatgc
    19081 aggaaattag ccagcagttg atgtccttgt tcgctaagaa tactttctgg atggaactgc
    19141 acaccttcca gatcccactg gcgatggcga atccccataa tctcgcgggt ttcgctccag
    19201 gccgtcacgt caaagcacgc tggtaatgag tcaggttcca ccaccagcga atggtagcgt
    19261 gtcacggtaa gtggatttgc cagcccccga aatacgccct caccgttatg tgtaatcggc
    19321 gaggttttgc cgtgcatgac ctttgcggcg cgcacaactt taccgccaaa tgcctgcgcc
    19381 attgcctgat gaccgaggca gacgccaaga atcggcaagc gcccggcata gtggcgaata
    19441 acgtcaaggg agatcccggc ttcatctggc gtacaggggc caggtgagat gacaattttt
    19501 tgtggtttaa gggcgtcgat atccgccagc gtcaacgcat cgttgcgctt aaccagcaca
    19561 tccgccccca gttcacaaaa gtactggtag aggttccagg taaaagaatc gtagttatct
    19621 ataagcagga tcatggcggc tccggtacga aagaaccacg ctattttact cagattcccc
    19681 ggcttcgctt accactttgc taaatatcat ctgcagcgcg gtgagatccc ccattgcccc
    19741 actctgattt gcctgattcc aggcttcttt ttcgataccc tgccagctca gttgatatcc
    19801 ggcatgaatc gccagttgct cgaagaagat ccgctgtgcc agtccgcttc ccacacggaa
    19861 gggatgcagc acgttgattt cacaatagta atgcgccagc cgctcgacga acttcgcttt
    19921 ctccaggcca accagataac cttcttcctc cagatcctgc atcagggcat tgccctcttt
    19981 ttcgatataa gcaaagtggc agaacggcgt atcgccctga taaatatcaa cttcacgcag
    20041 ttgccccgcc cagtcgaaaa tatcctgata cagctggcga tggatagtgc gcaaatgcgg
    20101 taaaccgcgc accagcggac caagctcaat ggtcgcagca cgcagcgccg tcatttcgta
    20161 agcggcctgt tccagccgct gctgctggtg gatgttcagc cggttacgca tgatatcaag
    20221 gccaggataa agatacggat cgcgcccttc gccgaattta tcgctcatag tgcctcctca
    20281 actcttcaag gcgcgctaaa gcctctgcgg cagtaagagt gactaaaggc atctcgacgc
    20341 cttcaagacg gcgactggcc tggaaattac gattacgctg aagctcccag agacgggact
    20401 tttgcttatc ggtgagtttc ttcacttgat gcctccctga acgtgtccat ttgccacaag
    20461 tataagcggc aaatccgggt tacgccggga gagcagaagc gcgggcaaga ttgcccgcgc
    20521 gaaaaatcat tacggcagga ctttagcgga aaggataact accggttttg acggcacatt
    20581 ctggtacgga ccaacgtcat gagtcggcac ctgggaaatc ttatcggcaa cgtccatgcc
    20641 tttcaccact ttaccaaata ccgcgtaacc gaaatcacgc tgaccatggt caaggaaggc
    20701 gttatcggca acgttgataa agaactggct ggtggcgctg tctttgtcag cggtacgcgc
    20761 catcgcgatg gtgccacgcg tgttgcgcag gccgttatcg gcttcatttt tgattggcgg
    20821 gtttggtttt ttctgctgca tctgctcggt gaaaccgccg ccctgaatca taaagccagg
    20881 aatgacgcgg tgaaaggtag tgttgttata aaaaccgctg ttcacataat cgacaaagtt
    20941 ttgcacagac actggcgctt tttgtttatc cagctccagt tcgatgttac cagctgaggt
    21001 tgtcaacaat acgtgcgggt cccctttcgc tgccattgct gcgggagaaa gagcagaaag
    21061 agcgaaaaca gcagccatcg ccgccagggt cgatttgaac atgagatttc cttaacaaac
    21121 agagcagaaa aaaagcaagt gaagagattc taaatagcct tcaagaacaa ggccatccct
    21181 ttacctaatt ttacgtatct gaaatatatg taacctgacg atcacttttt attccgtgat
    21241 caaaatcacc tcttaaaatg caatttagca accgattgca ataaaacatt taaacagatc
    21301 acaaaatcac ctaaaatcgc ccgctcgcgg catatcccgc tatgccattt tatttttgaa
    21361 cgcaggccaa tcatgactaa cagcaatcgc atcaagctca catggattag ctttctctcc
    21421 tacgcactga ctggtgcgtt ggttattgtc accgggatgg tgatgggaaa tatcgccgat
    21481 tatttcaatc tgcctgtttc cagtatgagt aacaccttca ccttcctcaa cgccggcatt
    21541 ttaatctcta tcttcctcaa cgcctggctg atggaaatcg tcccgttgaa aacgcagtta
    21601 cgttttggct ttctcctgat ggtgctggcg gttgccggtt tgatgttcag ccacagcctg
    21661 gcgctgttct cggcggcgat gttcattctc ggggtggtca gcggcatcac catgtcgatt
    21721 ggtacattcc tggtaacaca aatgtatgaa gggcgtcagc gcggttcccg cctgttattt
    21781 accgactcct tcttcagtat ggctgggatg attttcccaa tgatcgccgc gtttctactg
    21841 gcgcgcagca ttgagtggta ctgggtttat gcctgcatcg ggctggtgta tgtcgctatt
    21901 tttattctga ccttcggctg tgagttcccg gcgctgggca aacatgcgcc aaaaacggat
    21961 gctccggtag agaaagaaaa gtgggggatc ggcgtactgt ttctctctgt tgcggcgctg
    22021 tgctacatcc tcggtcagtt aggttttatc tcctgggtgc ctgagtatgc caaaggcctg
    22081 ggcatgagcc tgaacgacgc gggcacgctg gtgagtaact tctggatgtc atacatggtc
    22141 ggcatgtggg cgttcagctt tattcttcgc ttctttgatt tgcaacgcat tctgaccgta
    22201 ctggctggtc tggctgcgat tctgatgtac gtctttaaca ccggaacacc agcacatatg
    22261 gcgtggtcaa ttctcgctct gggcttcttc tccagcgcga tctataccac catcatcacc
    22321 ctgggttcac agcagaccaa agtaccgtcg ccaaaactgg ttaactttgt cctgacctgc
    22381 ggaaccatcg gtactatgtt gacctttgtg gttaccggcc cgattgttga acatagcggt
    22441 ccgcaggcgg cactgcttac agcaaacggt ctgtacgctg tcgtctttgt gatgtgcttc
    22501 ctgttaggtt tcgtcagccg tcaccgtcag cataacaccc tgacctctca ttaattgctc
    22561 atgccggacg gcactctcgt cgtccggcct tttcctctct tcccccgcta cgtgcatcta
    22621 tttctataaa cccgttcatt ttgtccattt tttgcacaaa catgaaatgt cagacaattc
    22681 cgtgacttaa gaaaatttat acaaatcagc aatatacccc ttaaggagta tataaaggtg
    22741 aatttgattt acatcaataa gcggggttgc tgaatcgtta aggtaggcgg taatagaaaa
    22801 gaaatcgagg caaaaatgag caaagtcaga ctcgcaatta tcggtaacgg tatggttggc
    22861 catcgcttta tcgaagatct tcttgataaa tctgatgcgg ccaactttga tattaccgtt
    22921 ttctgtgaag aaccgcgcat cgcttatgac cgcgtacacc tctcgtctta cttctctcac
    22981 cacaccgccg aagagctgtc gctggtgcgc gaaggcttct acgagaaaca cggcatcaaa
    23041 gttctggtcg gcgaacgcgc tatcaccatc aaccgtcagg agaaggtgat tcactccagc
    23101 gccgggcgca ccgtttttta tgacaagctg atcatggcga ccggttccta cccgtggatc
    23161 ccgccaatca aaggttctga tactcaggac tgctttgtct atcgcactat tgaagacctc
    23221 aacgccattg aatcctgcgc ccgtcgcagc aaacgcggtg ccgttgttgg tggcggcctg
    23281 ttaggtctgg aagccgctgg cgcgctgaaa aacttaggta ttgaaaccca cgttatcgaa
    23341 tttgccccta tgctgatggc ggaacagctt gatcagatgg gtggcgagca gctgcgtcgc
    23401 aaaatcgaaa gtatgggcgt gcgcgttcac accagcaaaa acacccttga gattgtgcag
    23461 gaaggtgtcg aagcgcgtaa aaccatgcgt tttgccgacg gcagcgaact ggaagtcgac
    23521 tttatcgtct tctctaccgg tatccgtccg cgcgataagc tggcaaccca gtgtggtctg
    23581 gacgttgctc cgcgtggggg tatcgtcatt aatgattcct gccagacttc cgatccggat
    23641 atctacgcca tcggtgaatg cgcaagctgg aacaaccgtg tatttggtct ggtagcacct
    23701 ggctacaaaa tggcgcaggt cgccgttgac catattctcg gtagcgaaaa cgcctttgaa
    23761 ggtgctgacc ttagcgccaa gctgaaactg ctgggcgtag acgtaggcgg tattggtgat
    23821 gcgcacggtc gcacgcctgg cgcacgtagc tacgtttacc tcgacgaaag taaagagatc
    23881 tacaaacgcc tgattgtcag cgaagacaac aaaaccctgc tcggtgcggt actggtgggc
    23941 gataccagcg actacggcaa cctgctgcaa ctggtgctga atgctatcga gctgccggaa
    24001 aacccggatt ctctgattct gccggcgcac tcgggtagcg gtaagccgtc tatcggtgtt
    24061 gataaactgc cggacagcgc gcaaatctgc tcctgcttcg acgtcaccaa aggtgatctg
    24121 attgctgcca tcaacaaagg ctgccacacc gttgcggcgc tgaaagctga aaccaaagcg
    24181 ggtactggct gcggtggctg tatcccgctg gttactcagg tactgaacgc ggaactggcg
    24241 aaacagggca tcgaagttaa caacaacctg tgcgaacact ttgcttattc gcgtcaggaa
    24301 ctgttccatt tgatccgcgt tgaaggcatt aaaaccttcg aagaactgct ggcgaaacac
    24361 ggcaaaggct acggttgtga agtttgtaaa ccgaccgtcg gttcgctgct ggcctcctgc
    24421 tggaacgaat acattctgaa gccggaacat actccgctgc aggattctaa cgacaacttc
    24481 ctcgctaaca tccagaaaga cggcacctac tcggtgatcc cgcgttctcc gggcggtgaa
    24541 atcaccccgg aagggctgat ggcagtgggt cgtatcgcgc gtgaatttaa tctctacacc
    24601 aaaatcaccg gctcccagcg cctggcgatg tttggcgcac agaaagacga tctgccggaa
    24661 atctggcgtc agctgattga agctggcttc gaaaccggtc atgcctatgc gaaagcactg
    24721 cgtatggcga aaacctgcgt gggtagcacc tggtgccgct acggtgttgg cgatagcgtc
    24781 ggcctcggcg tggaactgga aaaccgctac aaaggcatcc gtacgccgca caaaatgaag
    24841 ttcggtgtct ccggctgtac ccgtgaatgt tcagaagctc agggtaaaga cgtgggtatt
    24901 atcgccactg aaaaaggctg gaacctgtat gtttgcggta acggcggcat gaaaccgcgt
    24961 catgcggatc tgctggcagc ggatatcgat cgcgaaacgc tgatcaaata tctcgaccgc
    25021 ttcatgatgt tctacatccg taccgccgac aaactgacgc gtaccgcgcc gtggttagaa
    25081 aacctcgaag gcggcatcga ttacctgaaa gcggtgatca ttgacgacaa actggggctg
    25141 aacgctcatc tggaagaaga gatggcgcgc ctgcgtgaag cggtagtgtg tgagtggact
    25201 gaaacggtca atacaccgtc cgcgcagact cgcttcaaac acttcatcaa cagcgacaag
    25261 cgtgacccga acgtgcagat ggtgccagag cgcgaacagc accgtccggc aacgccgtat
    25321 gaacgtatcc cggtaactct ggtggaggac aacgcatgag ccagtggaaa gacatctgca
    25381 aaatcgatga catcctgcct gaaaccggcg tctgcgcgct gttaggcgac gagcaggtcg
    25441 cgattttccg cccgtatcac agcgaccagg tgtttgcgat cagcaacatc gatccgttct
    25501 tcgagtccag cgtgctgtca cgcgggctga ttgcggaaca ccagggcgag ctgtgggtcg
    25561 ccagcccgct gaaaaaacag cgttttcgct taagtgatgg cttgtgcatg gaagacgaac
    25621 agttttccgt caaacattac gaagcacgag tgaaagacgg cgtggtgcag ctgcgcggtt
    25681 aatgttttaa cgggaggcgc aatgcctccc ctttttgcat ggtcctgtaa tgaccttcgg
    25741 tatattgcag gacatttttt aaactttttg ttttattttt ttgtttttat tttttaaagg
    25801 ataatcaaat gtttacagac actattaata agtgtgcggc taacgctgcg cgcattgcac
    25861 gcctgtcggc aaataacccg ctcggctttt gggtcagctc cgccatggcg ggcgcgtatg
    25921 tgggtcttgg gatcatcttg attttcacgc tcggtaattt gctcgacccg tccgtacgtc
    25981 cgctggtgat gggcgcgacc tttggtatcg ccttaacgct ggtgattatc gccggttctg
    26041 aactgttcac cggacacacc atgttcctca cctttggggt aaaagcgggc agcatcagcc
    26101 acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg taacctggtc ggttccgtct
    26161 tcgttgccat gctctatagc tggggcggcg gtagcctgct gccggtagat accagcatcg
    26221 ttcactccgt cgcgctggct aaaaccactg caccggcaat ggtactgttc ttcaaaggtg
    26281 cattgtgtaa ctggctggtt tgcctggcaa tctggatggc gctgcgcact gaaggggcgg
    26341 cgaaatttat cgctatctgg tggtgtctgc tggcatttat cgcgtccggc tacgagcact
    26401 ctatcgctaa catgacgctg ttcgcgctct cctggttcgg caaccacagc gaagcctaca
    26461 cgctggcggg tattggtcat aacctgctgt gggtgacgct gggtaatact ttatcaggtg
    26521 ccgtattcat gggattgggt tattggtatg ctacgccgaa agcgaatcgt ccggttgcgg
    26581 acaaatttaa tcaaactgaa acggctgccg gttaattact aaggggtttt tacgtggatc
    26641 atttgcctat attttgccaa ttacgcgatc gcgactgtct gattgtcggc ggtggtgatg
    26701 tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc tcgcttaacg gtgaatgcat
    26761 tagcgtttat tccacagttc accgcatggg cagatgcagg catgttaacc ctcgtcgaag
    26821 ggccatttga tgaaagcctt ctcgacacct gctggctggc tattgcagcg acggatgatg
    26881 acgcgcttaa ccagcgcgtc agcgaagccg ctgaagctcg tcgcatcttc tgtaacgtgg
    26941 tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat tattgaccgc tcaccgctca
    27001 tggtcgcggt ctcctctggc ggcacctctc cggttctggc acgcctgttg cgcgaaaaac
    27061 tcgaatcact gctgccgttg catctgggcc aggtagcgaa atacgccggg caattacgcg
    27121 ggcgagtgaa acaacagttc gccacgatgg gtgagcgtcg ccgtttctgg gagaaattgt
    27181 tcgttaacga ccgcctggcg cagtcgctgg caaacaacga tcagaaagcc attactgaaa
    27241 ccaccgaaca gttaatcaac gaaccgcttg accatcgcgg tgaagtggtg ctggttggtg
    27301 caggtccggg cgatgccggg ctgctgacgc tgaaaggact gcaacaaatt cagcaggcag
    27361 atgtggtggt ctacgaccgt ctggtttctg acgatattat gaatctggta cgccgcgatg
    27421 ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg cgtaccccag gaagagatta
    27481 accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt ggtgcggctg aaaggtggcg
    27541 atccgtttat ttttggccgt ggtggcgaag agctggaaac actgtgcaac gcgggtattc
    27601 cgttctcggt ggttccgggt attaccgcag cttctggttg ctctgcctat tcgggtattc
    27661 cgctcacgca tcgcgattat gcccagagcg tgcgcttaat taccggacac ttaaaaactg
    27721 gtggcgagct ggactgggaa aacctggcgg cagaaaaaca gacgctggtg ttctatatgg
    27781 ggttgaatca ggccgcgact attcagcaaa agctgattga acacggaatg ccaggcgaaa
    27841 tgccggtggc aattgtcgaa aacggtacgg cagtcacgca gcgcgtgatt gacggtacgc
    27901 tcacacagct gggagaactg gcgcagcaaa tgaacagtcc atcgctaatt attattggtc
    27961 gggttgttgg cctgcgcgat aaactgaact ggttctccaa ccattaattt aacccgggcc
    28021 agagaattct ggccttctta atgactcttt ttatatattc agcaaataaa acataacccc
    28081 ttaataaata ttatctgcta atgggttata ttcaccgtta cagcgactta caaaattaag
    28141 tcattcgctc actgacgcca a
//
LOCUS       DAFNBJ010000055        27826 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000055 DAFNBJ010000000
VERSION     DAFNBJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.055"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..184
                     /locus_tag="L2X49_002189"
     CDS             <1..184
                     /locus_tag="L2X49_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HBN4797271.1"
                     /translation="SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPI
                     EAGEFKATSTFQVTYP"
     gene            297..1091
                     /gene="panB"
                     /locus_tag="L2X49_002190"
     CDS             297..1091
                     /gene="panB"
                     /locus_tag="L2X49_002190"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBN4797272.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRTAVRQYMAEVESGVYPGEEHSFH"
     gene            1103..1954
                     /gene="panC"
                     /locus_tag="L2X49_002191"
     CDS             1103..1954
                     /gene="panC"
                     /locus_tag="L2X49_002191"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBN4797273.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2028..2930)
                     /gene="rpnC"
                     /locus_tag="L2X49_002192"
     CDS             complement(2028..2930)
                     /gene="rpnC"
                     /locus_tag="L2X49_002192"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBN4797274.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3204..3584
                     /gene="panD"
                     /locus_tag="L2X49_002193"
     CDS             3204..3584
                     /gene="panD"
                     /locus_tag="L2X49_002193"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBN4797275.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3588..4817)
                     /locus_tag="L2X49_002194"
     CDS             complement(3588..4817)
                     /locus_tag="L2X49_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBN4797276.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(4881..5321)
                     /locus_tag="L2X49_002195"
     CDS             complement(4881..5321)
                     /locus_tag="L2X49_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN4797277.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5426..6196)
                     /locus_tag="L2X49_002196"
     CDS             complement(5426..6196)
                     /locus_tag="L2X49_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4797278.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6193..7119)
                     /locus_tag="L2X49_002197"
     CDS             complement(6193..7119)
                     /locus_tag="L2X49_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN4797279.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7228..7890
                     /gene="can"
                     /locus_tag="L2X49_002198"
     CDS             7228..7890
                     /gene="can"
                     /locus_tag="L2X49_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBN4797280.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(7931..8467)
                     /gene="hpt"
                     /locus_tag="L2X49_002199"
     CDS             complement(7931..8467)
                     /gene="hpt"
                     /locus_tag="L2X49_002199"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBN4797281.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8673..11063
                     /gene="gcd"
                     /locus_tag="L2X49_002200"
     CDS             8673..11063
                     /gene="gcd"
                     /locus_tag="L2X49_002200"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBN4797282.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWLSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKNPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11265..12815)
                     /gene="cueO"
                     /locus_tag="L2X49_002201"
     CDS             complement(11265..12815)
                     /gene="cueO"
                     /locus_tag="L2X49_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBN4797283.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQHGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12981..13328
                     /locus_tag="L2X49_002202"
     CDS             12981..13328
                     /locus_tag="L2X49_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBN4797284.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13434..14300
                     /gene="speE"
                     /locus_tag="L2X49_002203"
     CDS             13434..14300
                     /gene="speE"
                     /locus_tag="L2X49_002203"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBN4797285.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLTHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14316..15110
                     /gene="speD"
                     /locus_tag="L2X49_002204"
     CDS             14316..15110
                     /gene="speD"
                     /locus_tag="L2X49_002204"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBN4797286.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15148..15510)
                     /locus_tag="L2X49_002205"
     CDS             complement(15148..15510)
                     /locus_tag="L2X49_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBN4797287.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15685..18282)
                     /gene="acnB"
                     /locus_tag="L2X49_002206"
     CDS             complement(15685..18282)
                     /gene="acnB"
                     /locus_tag="L2X49_002206"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBN4797288.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18637..20451
                     /locus_tag="L2X49_002207"
     CDS             18637..20451
                     /locus_tag="L2X49_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBN4797289.1"
                     /translation="MKMTLPFKPHVLALICSVGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHRDGNGWQHNGDNINIDVNNFNR
                     ITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSATQLPAPTRDSQRQ
                     AAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFSRRQPLTQQQKDAA
                     RQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQAVREKMQTNPQIQ
                     QRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQ
                     ESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
     gene            complement(20522..21946)
                     /gene="lpdA"
                     /locus_tag="L2X49_002208"
     CDS             complement(20522..21946)
                     /gene="lpdA"
                     /locus_tag="L2X49_002208"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBN4797290.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22154..24046)
                     /gene="aceF"
                     /locus_tag="L2X49_002209"
     CDS             complement(22154..24046)
                     /gene="aceF"
                     /locus_tag="L2X49_002209"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBN4797291.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24061..26724)
                     /gene="aceE"
                     /locus_tag="L2X49_002210"
     CDS             complement(24061..26724)
                     /gene="aceE"
                     /locus_tag="L2X49_002210"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBN4797292.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26885..27649)
                     /gene="pdhR"
                     /locus_tag="L2X49_002211"
     CDS             complement(26885..27649)
                     /gene="pdhR"
                     /locus_tag="L2X49_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBN4797293.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         6658 a         7600 c         6864 g         6704 t
ORIGIN      
        1 ttctgtttat aaagattacg acccgagagg caaagacgat acaacaggag gagtttaccc
       61 ggatcaagat acaggtataa cataccctct ccatttccag gccacgctac aacaggatgg
      121 aactatacca atagaagctg gtgagtttaa agccaccagt actttccagg taacctaccc
      181 ttaataagtc cactgcaccg ccatcatctg gcggtgcagt aattgataaa tcattgcccc
      241 caccaatgtg actgataaaa tgccaggcaa cacgacatca tttatcagga tacgttatga
      301 aaccaaccac cattgcttca cttcagaaat gtaagcagga taaaaagcgt ttcgcgacca
      361 tcaccgctta cgactacagc ttcgcgaaac tatttgctga tgaaggactt aacgtcatgc
      421 tggtaggcga ctcgctgggc atgacggttc agggtcacga ctccaccctg ccggtgaccg
      481 ttgctgatat cgcctaccac actgccgccg tacgtcgcgg cgcgccaaac tgcctgctgc
      541 tggctgatct gccgtttatg gcgtatgcca cgccggaaca agcctttgaa aacgccgcaa
      601 cggttatgcg tgccggtgcc aatatggtca aaattgaggg cggtgagtgg ctggtcgaaa
      661 ccgtaaaaat gctgaccgaa cgtgccgttc ctgtatgtgg tcacttaggt ttaaccccac
      721 agtcagtgaa tattttcggt ggctacaaag ttcaggggcg cggcgatgaa gcgggcgatc
      781 aactgctcag cgatgcatta gccttagaag ccgctggggc acagctgctg gtgctggaat
      841 gcgtgccggt tgaactggca aaacgtatta ccgatgcact ggcgatcccg gttattggca
      901 ttggcgcagg caacgtcact gacgggcaga tcctcgtgat gcacgacgcc ttcggcatta
      961 ccggcggtca cattcctaaa ttcgctaaaa atttcctcgc cgaaacgggc gacatccgca
     1021 cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt ttatccgggc gaagaacaca
     1081 gtttccatta aggagtcacg ttgtgttaat tatcgaaacc ctgccgctgc tgcgtcagca
     1141 aattcgccgc ctgcgtatgg aaggcaagcg cgtggcgctg gtgcctacta tgggtaatct
     1201 gcacgatggc catatgaagc tggtcgacga agccaaagcc cgcgccgatg tggtcgtcgt
     1261 cagtattttc gttaacccaa tgcagtttga ccgcccggaa gatctggctc gttatccacg
     1321 gaccttgcaa gaggactgcg aaaagctaaa caaacgtaaa gtggatttag ttttcgcccc
     1381 ttcggtaaaa gagatctacc cgaacggtac tgaaacccac acttacgttg acgttcctgg
     1441 cctttcgacc atgctggaag gtgccagccg tccgggacat tttcgcggcg tttcgactat
     1501 tgtcagcaag ctgttcaacc tggtccagcc ggacatcgcc tgcttcggtg aaaaggactt
     1561 tcagcaactg gcgctaatcc gcaaaatggt tgccgatatg ggcttcgata ttgaaattgt
     1621 cggcgtcccg attatgcgcg ccaaagatgg ccttgcgctc agttcccgta atggttatct
     1681 gacggcggaa caacgcaaaa ttgcgcccgg tctgtacaaa gttttaagtt caattgccga
     1741 caaattgcag gctggggaac gggatctcga tgaaattatt accattgcgg ggcaagaact
     1801 gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgc gatgccgaca cattgctgga
     1861 agtttctgaa accagcaaac gggcagtaat tctggtagcc gcctggcttg gcgatgctcg
     1921 cctgatcgac aacaaaatgg tcgagctggc gtaatactta actggcgcta cggctgatgg
     1981 cgccagttat taatttaccc cacgtcatct gtcataacac aggtaactta aataaggtta
     2041 attaccttat caatctcagc aagaggtaaa tttgccatct ctgcaacgtc ttcccgagac
     2101 attcctttac ttagaagacg cagggcgaat tcttgccttt cctcctgtct tccctgctga
     2161 atccccttct caatcccttt ctcttcaaac cactgcgcca gcgtcatcat agactcccct
     2221 cccgtttccc tgtctctcag cacaccataa aacaaatccg cttgttcagt atgaccgcgt
     2281 tgcagcatat agttttgcat ggcaactaac tgacttccgc tagtgtaccc ttcgtcaatc
     2341 agcgtgacca gttgctccag caataacatt aagtcgcgct ggcgaatatg tttttgcagt
     2401 agttcgagaa tcgcaatccg ccgatgttgc atgatttcgt catccggtgt gatggtgata
     2461 tccaccagcg ggaaaggact gttatagacg cgtcgtgcca gctccggcga gtaaaatata
     2521 tcaaaccagc acattgagag cggataaggt gtggcctcgc cctgataaaa cagtatcggc
     2581 accaccagcg gcagcttatc gtgatccgcc tccagatgcc ggtgcatggc agcaatagaa
     2641 taacgcatca tgcgaaaggc cattttctta tccggcttgc tttggtgttc aatcacaaca
     2701 tgcagataac cgggattgcc ctgcatttgc acggaataga gcacgtccgt gctgtgtcct
     2761 ttcaggctct cttcaatgaa actccccgac tctaaatgaa gcgtgttgag gtcacaaagt
     2821 tcgcgtaatt ccactggcaa atgtatctcc agaaagtcgc gagccgtctc cgcatgcatt
     2881 aaaaattgtt taaataccgc gtcatgcggt gtggtactcg gtgcatccat cttccctgtc
     2941 ttccgtgttg tcttgcgtga aggcagtatc cagtcaaggc gcgccgtcgt cagccgcctc
     3001 tttagtgttt accgagcagc gttcagagaa atttcaattc gttacaggcg atacatggca
     3061 cgcttcggcg cggtacgcaa actgctgacg caaatgccat cagatgagta gacactcatc
     3121 aaaaatcggg caatactgcg tgagaatttc ctcaaagcag gcaccgcctg cttcgttaac
     3181 gacagggtag aaaggtagaa gttatgattc gcacgatgct gcagggcaaa ctccaccgcg
     3241 tgaaagtgac tcatgcggac ctgcactatg aaggttcttg cgccattgac caggattttc
     3301 ttgacgcagc cggtattctc gaaaacgaag ccatcgatat ctggaatgtc accaacggca
     3361 agcgtttctc cacttatgcc atcgcggcag aacgcggttc gagaattatt tctgttaacg
     3421 gtgcggcggc ccactgcgcc agcgtcggcg atattgtcat catcgccagc ttcgttacca
     3481 tgccagatga agaagctcgc acctggcgac ccaatgtcgc ctattttgaa ggcgacaatg
     3541 aaatgaaacg taccgcgaaa gcgattccgg tacaggttgc ttgattgtta tccctgcggc
     3601 tggttactca ccagccgcga catcgtctcc agcgaatccg ttcttaagat ataaagtcgt
     3661 tttagtaaca acggattatc ccccggtttt actttgcctt tcatggttgt caccgccagg
     3721 tgaaatccgg catcgtttgc tgccttcacg gctttgtcat taaatccgcc aaacggatac
     3781 gaaagatacc agacatgcgg attaaattgc gccagagcgc ggcgtgaacg tgcaaaatca
     3841 aacagaatat tgtgctcact acggctcagt aatatgggtc ggcgatagcc atccacccga
     3901 tgcaaaaaat gggtatgtga ctgaaaatca aaaacatcgc gaatttcgtt aagctcagaa
     3961 acgctcataa attgcagcga ttttgggttc cacttctgcg ggtgacgttt gatgcgcgag
     4021 gtaacaacaa acgccgtcgc cttcatgcca tattgtttca atacaggata cgcatagcgg
     4081 ctcaccgact tgaggccatc atcaaaggta atcaccaccg ctcgcgcagg gagattgatc
     4141 ttattcttca cgtaaccttc cagctgcgcc atgctcagtg tcgcgtatcc cctgtcgcgc
     4201 agccaggcca tctggttatt gaaagcgcgt accgaggtgg tcgtcgaagt atggcgaaaa
     4261 cgggtgtttt cttcatcgcg cagaatatgg tgataggtta gcaccggcag gccattatcg
     4321 ggttgggcat ccagcgcgct gatataggcg agtcgatcgc caatacggat ctgataccag
     4381 gtttgattta acctgtcttt cagtttatgc aaaatcgggt agcgcaaatt gtccgccagt
     4441 accccaaatg gcgcactgcc cgcactcggc gcgttataga ccggcgtatc tttccaggta
     4501 attaagttct gattactcag cggcttgttg aggtcaccca aaccgtcttc aactttttgt
     4561 cgcccctgaa ctggctcgag atgcccttta tcgataaacc ctttaccaaa gccaaaatta
     4621 aatgcgtaat aacttgcggc agtgggctcc acggcaatga tttgcccggc ccgaatattc
     4681 cccacggtca ccatcttgtc gccaatttgc gcccagatcg cagcattttc gatggtttgc
     4741 atataacggg caggtaacgc ggcactgaca cttgcggtaa acagcatcag caggagaata
     4801 acagcttgtt tatacatgaa aaaataaccg agggtgaaac aacgctttta ttgtagcaaa
     4861 agcgccacca gaatcgcggg ttaatactca tacaaattat gtttgaggac gaaaggcgga
     4921 tttttttgtt gttggtgcca aagactactc acctccggcg cacccagttc gacaatacgc
     4981 tcgcgaaact ctgaactggt catggtttca cggcaagcca tcatctgttc aattaacggt
     5041 aacgtgacac cagaaatcac ttcgcaacgg gagtgtttgt ggcttaataa tgaagccacg
     5101 cggtacggtg gcgctccggc tatatcggtt aagaagatga caccctcacc agagtccgct
     5161 tcatgcagag catcgcacat catgcggctg agcatattag agcttaaccc acgccagaaa
     5221 ttcacggccc ggcactgaag aagcgcccca tgttttttct ccagcgcatc cagtatctct
     5281 tgcgcccggt catcgtgaca ggtaattacc caacctaaca tcgccttcct ccttagccga
     5341 aaatcagtct accctgacca ttgttgataa aggatgatag ccgtcaaaac tcctctcccc
     5401 caaatccaga ggagaggaaa tagccttagc tacgcaaacc acgtccacgt tggatcagcg
     5461 accaacagat caaataaaac gccacaataa agaccaccag tacgccaaag gtagtgacca
     5521 gcggaacatc attgataccg aggaagccgt agcggaaacc actgatcata taaacgattg
     5581 ggttcaggtg cgacagccct tgccagaacg gcggcaacaa agtcagtgag taaaagaccc
     5641 cacccaaata cgtgagtggc gttaacacaa aggttggcac caggctgatg tcatcgaacg
     5701 ttttggcaaa cacaccgttc agcaaacccg caagggagaa caacaccgcc gtgagcacca
     5761 gggttaaggc aacgaatacc cacgaatgca cctgaaacgg cacaaaaaac agtgaaattg
     5821 ccgtcaccag aatgccaaca aacagaccac gcgccacgcc accgccgaca tatccggcaa
     5881 taatgacgtg agtcggaacc ggcgctacca gcagctcttc aatattacgc tggaacttgg
     5941 caccaaaaaa tgatgacgca acgttggcgt aggcattggt gatcaccgac atcatgatca
     6001 gccccggtac gatgaactgc atatagctga agccatgcat atcgccaata cgcgaaccaa
     6061 tcaggttacc gaagataata aagtaaaggg tcatggtgat gactggcggc accagcgtct
     6121 gcacccagat acgcataaag cgatggatct ctttcgccca gatgcttttt agcgccaccc
     6181 agtaaagatg catcatgcgc gatctccttg tttttcatta accagtgaaa caaacagctc
     6241 ttccagacgg ttagctttgt tacgcatact taatacctga atgccctgct cacttaactg
     6301 cgtaaatacg ctgttgatcc cctgctcacg cagcacttca acttccagcg tcgcggtatc
     6361 gaccagtcga tactgatagc catcgagctt cggtaacgga cttttcggtg cgagatcgag
     6421 aataaaggtt tccgatttca gcttcgccag cagcgccttc atcgaggtat tttccaccag
     6481 ctcaccgtgt tgaataatgc cgatattgcg gcacagcatt tctgcttctt ccaggtagtg
     6541 tgtggtgaga atgatggtgg tgcctttgtc gtttaaatcc ttcaaaaagc cccacattga
     6601 gcggcgaagt tcaatatcca cgcctgcggt cggttcgtcg agaatcagta gtttaggttc
     6661 atgcattaac gcacgggcaa tcattaaacg gcgcttcatc ccgccagata acatacgcgc
     6721 acgttcgttg cgttttcccc atagatcgag ttgtttaaga tatttttcgc tgcggatgta
     6781 cgcctctttg cgctccacac cgtagtaccc tgcctgattc accacaattt gctgcacggt
     6841 ttcaaacggg ttgaagttaa attcctgcgg caccagtccc aactgacgtt tagcgttcac
     6901 gacatctttc tcgagatcgt aaccaaatac gctgacccgc ccggaggttt tatttaccag
     6961 agagctgata ataccgatag tggtcgattt cccggccccg ttcggcccga gaagcgcata
     7021 aaaatcaccc gcttcgacct gcaaatctat tccacgaagc gcctgaacgc cgcctggata
     7081 ggttttttta agctgttgaa gttccagtgc aatggtcata aatttttact taccttacgt
     7141 tcttacactt tatctgtggt ttaaatcgtc ccggagttgc cctatattag ccaaacgtaa
     7201 ttatttggtt acaggtcgtt aacctccatg aaagacatag atacactcat cagcaacaat
     7261 gcactatggt caaaaatgct ggtggaagag gatcccgggt tttttgagaa actggcacaa
     7321 gcgcaaaaac cgcgctttct atggattgga tgttccgaca gtcgcgttcc tgcagaacgt
     7381 ttaaccggtc ttgagccggg cgaactcttt gttcaccgta atgttgctaa cctggtcatt
     7441 cacaccgacc tgaactgcct ttccgtggtt cagtatgcag tggatgtact agaagttgaa
     7501 cacattatta tctgtggcca ctacggttgc ggcggcgtac aagccgcagt tgaaaacccg
     7561 gaactggggc ttatcaacaa ctggctgctg catatccgcg atatctggtt caaacatagc
     7621 tcattgctcg gcgaaatgcc gcaagagcgc cgtctggata ccttgtgtga actgaacgtc
     7681 atggaacagg tgtataacct gggccactcc accattatgc aatcagcgtg gaaacgcggg
     7741 cagaaagtta ccattcacgg ctgggcctat ggcattcacg acggcttgct gcgtgatctg
     7801 gatgtcacag cgactaaccg cgaaaccctt gagcaacgtt accgtcacgg gatttccaac
     7861 ctcaagctga aacacgccaa ccacaaataa aaatgccatg ccggatgcaa cacatccggc
     7921 aacttcacac ttactcgtcc agcagaatca ctttgccgat atacggcaga tgacggtaac
     7981 gctgtgcgta atcaatgccg taacccacca caaactcatc cgggatcgag aaaccgataa
     8041 attctaccgg gacgttcact tcacgacggg acggtttatc cagcagcgta caaatcgcca
     8101 gcgacttcgg ttcgcgcagg cttaagatct cacgcacttt cgacagtgta ttccccgagt
     8161 cgatgatatc ttcaacaatc agcacgtcct tgccacggat atcttcatcc agatctttga
     8221 ggattttcac atcacgggtg gtggacatgc cgctaccgta gctggaggcg gtcataaagt
     8281 cgacttcatg agatacctga acttcacggc acaggtccgc cataaacata aatgagccac
     8341 gcagcagacc aaccagcacc atatcgctgc cgctgtcttt gtaacgctca gtaatctgac
     8401 gacccagttc ggcgatacgc gctttaatct ccgcttcggg gatcattact tctacagtat
     8461 gtttcatatc tctaaccata tgatttaaaa acaaatcact tcgcacgggc aggttttggc
     8521 accgcaacaa agcgcaagac acgcagtata ccagtaaaac tattcattcg cggaatctgt
     8581 taataaagct cgaattgtga tgacgatcac acatgttaaa acctataatt aattgctact
     8641 aaaatattaa tgaattgaaa tggtgtctct ttatggcaat taacaataca ggctcgcgac
     8701 gattactcgt cacgctaaca gccctttttg cagcgctttg cgggctgtat ctactcattg
     8761 gcggaggctg gctggtcgcg attggcggct cctggtacta ccctatcgct ggtcttgtga
     8821 tgctcggcgt cgcctggatg ctgtggctca gtaaacgtgc cgcgctttgg ctgtacgcag
     8881 ccctgctgct cggcaccatg atttggggcg tctgggaagt tggtttcgac ttctgggcgc
     8941 tgactccgcg cagcgacatt ctggtcttct tcggcatctg gctgatcctg ccgtttgtct
     9001 ggcgtcgcct ggtcattcct gccagcggcg cagttgccgc actggtggtg gcactgctga
     9061 ttagcggtgg tatcctgacc tgggccggat ttaacgatcc gcaggaaatc aacggcacct
     9121 taagcgccga tgccacacct gccgaagcaa tctcccccgt tgccgatcag gactggcctg
     9181 cctatggtcg taatcaggaa ggtcaacgct tttcacctct gaaacaaatt aacgccgata
     9241 acgttcacaa gctgaaagaa gcctgggtgt tccgtactgg cgatgtgaag cagccgaacg
     9301 atccgggtga aatcaccaat gaagtgacgc cgattaaagt gggcgacacc ctttacctgt
     9361 gtaccgctca ccagcgactg tttgcgctcg atgccgccag cggcaaagag aaatggcatt
     9421 acgatcctga gctgaaaacc aacgagtctt tccagcacgt aacctgccgt ggcgtctctt
     9481 atcatgaagc caaagcagaa acggcttcgc cggaagtgat ggcggattgc ccgcgtcgta
     9541 tcattcttcc agtgaatgat ggtcgcctga ttgcgattaa cgctgaaaac ggcaaactgt
     9601 gcgaaacctt cgccaataaa ggcgtgctta atctgcaaag caatatgcca gacaccaaac
     9661 cgggtctgta tgaaccgact tcgccaccga ttatcaccga taaaaccatc gtgatggcag
     9721 gttcagttac cgataacttc tcaacccgcg aaacgtctgg cgtgatccgt ggttttgatg
     9781 tcaacaccgg ggagctgctg tgggcttttg atccgggcgc gaaaaatccg aacgcaatcc
     9841 cgtctgacga acacaccttt acctttaact cgccaaactc gtgggcacca gcggcctatg
     9901 acgcgaagct ggatctggtg tatctgccga tgggcgtgac cacgccggat atctggggcg
     9961 gtaaccgcac accggaacag gaacgttatg ccagctcgat tctggcgctg aatgccacta
    10021 ccgggaaact ggcgtggagc taccagaccg ttcaccacga cctgtgggat atggatcttc
    10081 cggcacagcc gacgctggcg gacatcaccg ttaatggtca gaaagtgcca gttatttacg
    10141 ctccggcgaa aaccggcaac atttttgtgc tcgatcgtcg taatggcgaa ctggtggttc
    10201 cggcaccgga aaaaccggtt ccccaaggtg ctgccaaagg cgattacgtt acccctactc
    10261 aaccattctc ggaactgagc ttccgtccga cgaaagattt gagcggtgcg gatatgtggg
    10321 gagccaccat gtttgaccaa ctggtgtgcc gcgtgatgtt ccaccagatg cgctatgaag
    10381 gcattttcac cccgccatct gaacagggta cgctggtctt cccgggtaac ctggggatgt
    10441 tcgaatgggg cgggatttcc gttgatccaa atcgtgaagt ggcgattgcc aacccaatgg
    10501 cactgccgtt tgtttcgaaa ctgatcccgc gtggtcctgg taacccgatg gagcagccga
    10561 aagatgccaa aggcacgggt acggaatccg gcattcagcc acagtacggt gtaccgtatg
    10621 gtgtcacgct caacccgttc ctctcaccgt ttggtctgcc atgtaaacag ccagcatggg
    10681 gttatatctc ggcgctggat ctgaaaacca atgaagtggt gtggaagaaa cgtattggta
    10741 cgccgcagga cagtatgccg ttcccgatgc ctgtaccggt gccgttcaat atgggtatgc
    10801 cgatgctggg cgggccaatc tccacggcgg gtaacgtgct gtttatcgcc gctacggcag
    10861 ataactacct gcgcgcttac aacatgagca acggtgaaaa actgtggcaa ggtcgtttac
    10921 cagcgggtgg tcaggcgacg ccaatgacct atgaagtgaa tggtaagcag tatgtggtga
    10981 tctccgcagg cggtcacggt tcatttggta cgaagatggg cgactatatt gtggcttatg
    11041 ccctgccgga tgatgtgaag taagacttgc tcagattgct gacaacgtgc gcgttgttca
    11101 tgccggatgc ggcgttaacg ccttatccag cctacaaaat tgtgcaaaat caatggattg
    11161 cacaggtagc gtaggcctga taagcgtagc gcatcaggca gatttgcgtt tgtcatcagt
    11221 ttaatgcccg gagagatccg ggcatatttc cgaatacggt ctttttatac cgtaaaccct
    11281 aacatcatcc ccgtatcttc atgctccagc agatggcagt gcgccatata agcatgttct
    11341 ttcggtgcat cgtgattaaa cttcaccagc acttcgctga cattaccttc taccttaacg
    11401 gtatctttcc agcccgcgcg atgagccgct ggcggtttgc cattttctga caagatacgg
    11461 aactgcgtgc cgtgaatatg gaacggatgc agcatcatgt cacccacgcc agagataacc
    11521 caacgttcgt actgcccttt cgccgccgca aacatcggct tattcatatc aaacgcctga
    11581 ccgttgattt tattggcatg gtggaaatcg aacttcccgc cgtggttcat atgattcata
    11641 ttgccgtgcc ccatatggcc catcatctgg ctgtgatcca tcccggccat cgcctgatcg
    11701 ccatatttct ccatcagcat ctgcataccc atcatatcga gcatcgggtc catagaaagt
    11761 tgcagcttgc gtaccgtcag cccttccagc gaaggtaacg caggcaggct acttaatgtg
    11821 tctggcaaag caccggaggc actaatagca atcggctgaa tccgcattac cggatgcggc
    11881 ttgtcaaacg gcgcaatcgc catccccatc tggctgaccg gcagcgtcac caggtcaaag
    11941 ggtttgttgt cgttaacctc caccagcact tcaaaacgct cgcccatcag caccggcagc
    12001 tcgttcacct tcaccggttc aggtagcaga ccaccgtcgc tggcaatcac ataaagcggg
    12061 cgattgtcgc tggtggcgaa attgagcgag cgggcattac agccattgag caaacgcagg
    12121 cgcagccaac cacgcggggc agcgtgttgc gggtagattg caccgttggt cagcaacgta
    12181 tcgccaaacc agcccacggc ggcggtcatc acatccagtt gataatcaat ctgcccgtcg
    12241 gcgctaaatt tcttatcctg aacgatcacc ggaacatcat cgatacccca ctgttttggc
    12301 agcattaatt tcaggatctc gtcatcttca attaccacca acccagccaa ccccatcgcc
    12361 acctgtcgcc cggtcttgcc atgttgatgt ggatggaacc agcaggtagc ggcaggttga
    12421 tcaacgttca acgtcaccga gcgcttgcca cctggcggaa taattccctg cgggccgccg
    12481 tccacttcac ccggtacttc cagcccgtgc cagtgcaacg tcgtctcttc cgtcagttgg
    12541 ttatagatat caaccgttac cgctttgccg tgctgtaatt tcaccgccgg ccccagcaga
    12601 ttgccgttat agccccaggt agttgcagtt ttcccgccaa aggtggactg gcctgcgcca
    12661 atagttaact gaatgcgatt acgggcatcg gtcgtgagca aatcagggat cggtaacgtt
    12721 gggcgttccg ccgcaaagac tgcgcggctc cacagcggca aggctgaagc cacacccagc
    12781 gcgacggaat attttaagaa atcacggcgt tgcatagtta tttccttatt cttaagcagg
    12841 cgaaacaaaa tcaaacgttg agcatagtcc ttccccttac gggaaggtca agccgcagac
    12901 acaatttaat cgacaataat ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct
    12961 ttaagcccct ggtagaagca atgaagacgt ttttcagaac agtgttattc ggcagcctga
    13021 tggccgtctg cgcaaacagt tacgcgctca gcgagtctga agccgaagat atggccgatt
    13081 taacggcagt ttttgtcttt ctgaagaacg attgtggtta ccagaactta cctaacgggc
    13141 aaattcgtcg cgcactggtc tttttcgccc agcaaaacca gtgggatctc agtaattacg
    13201 acaccttcga catgaaagcc ctcggtgaag acagctaccg cgatctcagc ggcattggca
    13261 ttcccgtcgc taaaaagtgc aaagccctgg ctcgcgattc cttaagcctg cttgcctacg
    13321 tcaaataatc cctgatacct ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta
    13381 gctattatgt tgcgcccttt ttttacgggt gttaacaaag gaggtatcaa cccatggccg
    13441 aaaaaaaaca gtggcatgaa acgctacacg accagtttgg gcagtacttt gcggtagata
    13501 acgttctgta tcatgaaaag accgatcacc aggatctgat catttttgag aacgctgcat
    13561 ttggtcgcgt aatggcgctg gatggcgtag tacaaaccac cgagcgcgac gagtttatct
    13621 atcatgagat gatgactcat gttccgctac tgacccacgg tcacgcgaaa catgtgctga
    13681 ttatcggcgg cggcgacggt gccatgctgc gtgaagtaac ccgacataaa aacgttgagt
    13741 caatcacgat ggtggaaatc gatgcgggtg tcgtatcgtt ctgccgtcag tatctaccca
    13801 accataacgc cggtagctac gacgatccgc gctttaagct ggtgatcgat gatggcgtca
    13861 atttcgttaa tcaaaccagc cagacctttg atgtcattat ctccgactgc accgatccta
    13921 tcggtcccgg cgaaagtctg ttcacctcag cattttatga aggctgcaaa cgttgcctga
    13981 atcctggcgg tatcttcgtc gcacaaaacg gcgtctgctt tttacagcag gaagaagcca
    14041 tcgacagcca tcgcaaactc agccattact tcagcgacgt tggcttttat caggcggcga
    14101 tcccgaccta ttacggcggt atcatgactt ttgcatgggc gacagataac gacgccttac
    14161 gccatctctc aaccgaaatt attcaggcgc gttttctcgc ttctggcctg aaatgccgtt
    14221 attacaatcc ggcaatccat acggcagctt ttgccttacc tcagtatctg caagacgcac
    14281 tggcttcaca gccgtcctaa ggagaagata agaaattgaa aaaactgaaa ctgcatggct
    14341 ttaataatct gaccaaaagt ctgagttttt gtatttacga tatctgctac gccaaaactg
    14401 ccgaagagcg cgacggttat attgcttata tcgatgaact ctataatgcc aaccgtctga
    14461 ccgaaatcct gtcagaaacc tgttccatta tcggggccaa tattcttaac atcgcccgtc
    14521 aggattacga accacagggt gccagcgtca ctattctggt gagtgaagaa ccggtcgacc
    14581 cgaaacttat cgacaaaaca gaacaccccg gcccactgcc agaaacggtg gttgcccatc
    14641 tcgataaaag tcatatttgc gtacatacct acccggaaag tcatcctgaa ggcggtttat
    14701 gtaccttccg cgccgatatt gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc
    14761 tgaattacct gatccaccag cttgagtccg atatcgtaac cattgattat cgcgtgcgcg
    14821 gttttacccg cgacattaac ggtatgaagc actttatcga ccatgagatt aattcgattc
    14881 agaactttat gtctgacgat atgaaggcgc tgtatgacat ggtggatgtg aacgtctatc
    14941 aggaaaatat cttccatacc aagatgttgc ttaaagagtt cgaccttaag cactacatgt
    15001 tccacaccaa accggaagat ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt
    15061 ggaaagaaat gcgcgagatt tattacgggc gcaatatgcc agctgtttaa cggctctggc
    15121 ggagctccca ggctccgcca gatttattta cttctgctgc acgaaattgc ggtaagccgc
    15181 cacgacctgc agaaaatcct caacgccgca tagcgacagg ctttcttcgt cgtagtagtt
    15241 catcccttct tccatttcat cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc
    15301 ttcaccgtcc atccacaggg tgtattcatg ccctgcccgt tgccaggacc gttcgctacc
    15361 tttcagtgcg tgcgctgctt gttccacttc atcaagcaaa gccaggtttt ctttcacctc
    15421 ttcattaaac cagtgcccga ccacttcatg ccccatggac atacgcacct ttaccactcc
    15481 ggtaatatcg cgcagaaatt cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc
    15541 gaaatcgcat tgcgccattg atgtaattat cgcaacctta ccggagaaaa acagcggaac
    15601 ggcgatggtt tagaaataaa aaatgcccgg gaatctcatt ccccgagcat tgtgtcgttt
    15661 atgcgcagcg cgtgcgctga ctttttaaac cgcagtctgg aaaatcaccc catcggcttt
    15721 ttcggtatac tgagaaagct ggttgaagtt cagataacga taagtatcaa cggcagtttt
    15781 atccacttgc gccacgtagg tctggtactc ttccggcgtc ggcagtttgc caatcagcgc
    15841 cgcaacagcc gccagttccg cagaagccag gaagacattc gcgccagtac ccagacggtt
    15901 cgggaagtta cgggtagagg tggaaaccac cgtcgcaccg tctgctacac gcgcctggtt
    15961 acccatacac agggaacagc cagggatctc gatacgcgca ccactcttac cgaagacgct
    16021 gtagtagcct tcttcagtca actgcgcggc gtccatacgg gttggcggtg ccacccacag
    16081 gcgggtcggc agttggcctt tgtgcgcatc cagcagttta cccgcagcac ggaagtgacc
    16141 gatgttggtc atgcaggaac cgataaatac ttcgtcgatc ttctcaccct gtaccgcaga
    16201 cagcggacgc gcgtcatccg ggtcgttcgg tgcacagagg attggctctt taatatccgc
    16261 cagatcgatg tcgatcactg ccgcgtattc agcatctgca tcggcttcca gcagctcagg
    16321 attcgccagc catttttcca tgccctgaat acgacgttcc agggtgcgac gatcgccgta
    16381 accttccgcg atcatccact tcagcaggac gatgttagag ttcaggtatt cgatgatcgg
    16441 ttctttgttc agcttgatgg tacaaccagc ggcagaacgc tcagcggacg catcggtaag
    16501 ctcaaaggcc tgctcaactt tcagatccgg cagaccttca atttccagaa tgcggccaga
    16561 gaagatgttt ttcttgcctt tcttctcaac ggtcagcaga ccttgtttga tggcatacag
    16621 cggaatggcg tgtaccagat cgcgcagggt gatgcccggc tgcattttgc ctttgaagcg
    16681 caccagaacg gattccggca tatccagcgg cattacgcct gttgcggctg caaatgccac
    16741 cagaccagaa cccgccggga aagagatacc gatcgggaaa cgggtatggg agtcacctcc
    16801 ggtaccgacg gtatccggca gcagcatacg gttcagccag gagtggatta cgccgtcacc
    16861 cggacgcagc gacacaccgc cacggttcat aatgaagtcc ggcagcgtgt ggtgcgtgtt
    16921 cacgtcaact ggcttcggat acgccgcggt gtggcagaaa gactgcatca ccaggtcagc
    16981 cgagaagccc aggcacgcca ggtctttcag ttcatcacgg gtcatcgggc cggtggtgtc
    17041 ctgagagcct acagaagtca ttttcggttc gcagtacgcg cccggacgaa tgcctttcac
    17101 gccacaggca cggcctacca ttttctgcgc cagcgagaag ccgcgatcgc tctcagcgac
    17161 atctttcgcc tgacggaaca catcactgtg cggcagacca agtgcttcac gcgctttggt
    17221 ggtcaggcca cgcccgataa tcagcggaat acggccacca gcacgcactt catcaatcag
    17281 cacgtcggtt ttcagttcga aggtcgccag cagttcgccg gtttcgtggt tacgcacttc
    17341 acctttgtac gggtaaacgt caatcacgtc gcccatgttc aggttagaga cgtcgacttc
    17401 gattggcagt gcacccgcgt cttccatcgt gttaaagaag atgggtgcaa ttttaccgcc
    17461 gaggcacaaa ccaccgccgc gtttgttcgg cacatgtgga atatcatcgc ccataaacca
    17521 cagaacggag ttagtggcgg atttacgcga agaacccgta cccacaacgt caccgacgta
    17581 cgccagcggg aaacctttct gttgcagagc ttcgatttgc ttgatcggac caacaacacc
    17641 aggctggtct ggctcaatac cttcacgggc gtttttcagc atcgccagcg cgtgcagtgg
    17701 gatatccggg cgtgaccacg catccggtgc cggagaaagg tcatcggtgt tagtttcgcc
    17761 agtgactttg aagacagtaa cggtcagttt ttcagccagc gccgggcgat tcaggaacca
    17821 ttcggcatcc gcccaggact gcataacctg cttcgcatat tcgttgcctg ctttcgcttt
    17881 ctcttctacg tcatagaagt tatcgaacat cagcagcgtg tgagaaagtg ctttggcagc
    17941 aataggtgcc agtttggcat catccagcgc gtcgatcagc ggatgaatgt tgtaaccacc
    18001 ctgcatggtg cccagcagtt cgatggcttt ttccggagtc agcagagggg atttggcttc
    18061 gcctttcgcg atagcagcca ggaagcctgc tttgacatag gcggcttcat cgacgcctgg
    18121 gggaacacgg ttggttaaca gatctaacag gaattcttct tcgcccgcgg gcgggttttt
    18181 cagcagctct acaagtgcgg ccatttggtt tgcatccagg ggtttgggcg caatcccctc
    18241 agcggcacgc tcagctacgt gcttacggta ttcttctagc acgacggttc tcctcgctct
    18301 cattgtcata gtgcggcagg cgattctctt cacgctcctg tgagacagca gtttgtaggg
    18361 taaatgccca gtaccgcaac gggcagaata gcaggatttt gacgatgtgt taatctgttt
    18421 acaaaaaagc aacataaaaa agtggctgaa tcgttaagga tggtctagag attgtttccc
    18481 tccgtaaaaa cttcgttaat tcgcatggtg ataatcacct ttctcaacaa atcccaagat
    18541 tagtcaaaat ttaaactacc gcctctttat actcggattc acagcacctg cgggtggcag
    18601 ttcgccgacc attgcgattt ccttgagatc cgaattatga aaatgacttt gccgtttaaa
    18661 ccccatgtgc tggcgctaat ttgcagtgtc gggctttgtg ccgcctctac cgggctatat
    18721 ataaaaagcc gcacagtgga agcgcctgtg gaaccgcaat cgacacaaca gactgcgcct
    18781 gacatcaccg cagttacgct tcctgcaacg gtttccgcac cgcccgtaac gcccgccgtc
    18841 gtcaaatccg cattcagcac tgcacaaatc gatcaatggg ttgcgcctgt cgcgctgtac
    18901 cccgattctc tgctttcaca agtgttaatg gcatcaacct atccggcaaa cgttgctcaa
    18961 gcagtgcaat ggtcgcacga taatccactt aaacaaggcg atgctgctat tcaggcggta
    19021 tctgaccagc cgtgggacgc cagcgttaaa tcactggtgg cctttccaca attgatggca
    19081 ttgatgggcg aaaacccgca atgggtgcaa aacctgggcg atgcttttct ggcgcagccg
    19141 caggacgtga tggactcggt acagcgattg cggcaactgg cgcaacaaac cggttcgctg
    19201 aagtcatcaa ccgaacaaaa agttattacc acaacgaaga aagctgtacc ggtaaaacag
    19261 acagtcacgg cacccgtcat accatccaat accgttttaa ctgccagccc cgtcattaca
    19321 gagcctgcaa caaccgtcat ttccattgag cccgccaatc ctgatgtggt ctatattccc
    19381 aactacaacc caaccgtggt ttacgggaac tgggccaata ctgcgtatcc gccggtttat
    19441 ctgccaccac cagccggaga accgtttatt gacagctttg tgcgcggatt cggctatagc
    19501 atgggtgttg ctaccacgta cgcattattc agcagcatcg actgggatga cgacgatcat
    19561 gaccatcatc atcgtgacgg taatggctgg caacacaacg gcgacaacat caatatcgac
    19621 gtcaacaatt tcaaccgtat caccggtgag catcttactg ataagaatat ggcatggcgg
    19681 cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc aaagcggttt
    19741 catcaaaccg atgtcaacgg cggaatgagc gccacgcagc tacctgctcc aacacgcgac
    19801 agccagcgtc aggcggcagc aagtcagttt cagcaacgaa cacacgccgc ccccgtcatt
    19861 acacgagata cccaacgtca ggcagcggca cagcggttta atgaagctga aaactatggg
    19921 agctatgacg acttccgcga cttcagccgt cgccaaccac tgacccagca acaaaaggac
    19981 gccgctcgtc agcgttatca gtcggcctcg cctgagcagc gccaggcagt ccgcgagaaa
    20041 atgcagacta acccacagat ccagcagcga agagacgcag cgcgtgagcg tattcagtcc
    20101 gcctcgcctg agcagcgcca ggcagtccgc gagaaaatgc agactaaccc acagatccag
    20161 cagcgaagag acgcagcgcg tgagcgtatt cagtcagcct cgcctgagca gcgccaggtg
    20221 tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa cgcccgccag
    20281 cgtgttcaat cagcatcacc tgaacaacgt caggtttttc gggaaaaagt tcaggagagc
    20341 cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga gcaacggtca
    20401 gcagtacgcg aacgtctctc tgagcgcgga gcaaggcgac aggaaaggta aattgcagac
    20461 gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg caaacgaaca
    20521 attacttctt cttcgctttc gggttcggca ggtcggtaat gctaccttcg aacacttctg
    20581 ccgccaggcc cacagactcg tgcagagtcg ggtgcgcgtg gatggtcagt gcgatgtctt
    20641 cagcatcaca acccatttcg attgccaggc cgatttcacc cagcagctcg ccgccgttgg
    20701 taccgacaat cgcaccaccg atcacacggt gagattcttt gtcgaaaatc agcttggtca
    20761 taccgtctgc gcagtcggaa gcgatagcac gaccagaagc agcccacggg aaggtggcgg
    20821 tttcatagct gatgcctttc tctttcgctt ctttctcagt cagacctacc catgcaactt
    20881 ctggttcggt ataggcgatg gacgggataa ctttcggatc gaagtagtgt ttcttaccgg
    20941 cgataacttc agcggcaacg tgaccttcgt gaacaccttt gtgtgccagc atcggctgac
    21001 cgacgatatc gccgatagca aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc
    21061 ggatgaaacc acggtcgtcc acttccacgc cagctttgcc tgcgtcgagg tttttaccgt
    21121 tcggcacacg accaatcgct accagcacgg cgtcgtaacg ctgcggttca gcgggtgctt
    21181 ttttgccttc catcgtcaca taaataccgt cttctttcgc ttcaacggcg gtaactttgg
    21241 tttccagcat caggttgaat ttcttgctga tacgcttggt gaagacttta acgatgtctt
    21301 tgtcagctgc cgggataacc tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg
    21361 cgtggtatac ggtgcccatt tccagaccga tgataccgcc acccattacc agcaggcgtt
    21421 ctggtacttc tttcagttcc agcgcgtcag tggagtccca gatacgcgga tcttcatgcg
    21481 gaataaacgg cagttggatc gggcgagaac ccgctgcaat gatcgcgttg tcgaagttga
    21541 tcacggtttt accgttctca ccttcaactt ccagggtgtt agccccggta aatttaccca
    21601 gaccgttgac cactttgact ttgcggcctt tcgccatacc agccagacca ccggtcagct
    21661 gattgatcac tttctctttc caggtacgaa tcttgtcaat atcggttttc ggttcgccga
    21721 agacgatacc gtgttcagcc agcgctttgg cttcttcgat aacttttgct acgtgcagca
    21781 gtgctttaga agggatacag ccgacgttca ggcaaacacc gccaagggtg ttgtaacgtt
    21841 ctacgattac ggtttccaga cctaaatcag cgcaacggaa ggcagcggag taacctgcgg
    21901 ggcctgcccc aagtaccacg acctgagttt tgatttcagt actcatcatg acctctatat
    21961 atttatctcc ggcgggtctg acgttttttt tatgacgccc attatccacc gggtcgttct
    22021 atccgtcggt attttacaaa attgttaaca atttttaaac aacaaacggc aaccgatttg
    22081 tctattcgct aataacctca atacattcat gagattacca gaaaaaagcc ggccgttggg
    22141 ccggctcttt tacttacatc accagacggc gaatgtcaga cagcgtgttg ttaatgatgg
    22201 taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg gtcgaaggag agagaaatcg
    22261 gcagcatcag acgcggcacg aactctttac cattccacac cggctccatc gcggacttgg
    22321 aaacgccgag gatagccact tccggcgcgt tcacaatcgg cgcgaagtgg gtagtaccca
    22381 ggccgccgat gctggagatg gtgaagcaac cgccctgcat ttcgcccgca gtcagcttac
    22441 cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga cagctcgatg atgcctttct
    22501 tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg ggtatccacc gccacaccga
    22561 tgttgatgta tttcttcagg gtcagacgct gaccgtcttc cgacagcgaa ctattgaagc
    22621 gaggcatctg ctcaagagct gcagcaacgg ctttcatgat gaagacaacc ggggtgatct
    22681 tcacatccag cttacgtttc gccgcttctt cgttctgctg tttacggaac gcttccagct
    22741 cggtgatatc ggttttgtcg aagtgagtaa catgcgggat catcacccag ttacggctca
    22801 ggttcgcacc agaaattttc tggatacggc ccagttccac ttcttcgatt tcaccaaact
    22861 tgctgaagtc caccttcggc cacggcagca tgcctgggat accgccgcca gtcgccgccg
    22921 gagctgcttc tgcacgtttg atagcttctt tcacgtaagc ctgaacgtct tcgcgcagga
    22981 tacgaccttt acggccagtg cccttcactt tcgccaggtt aacgccaaac tcgcgtgcca
    23041 gacggcggat cagcggagtc gcgtgaacgt aagcgtcgtt ttctgcaaat tcagatttgc
    23101 cttccgcttt cgcagccggt gctgctgccg gagcttcagc ttttgctgcc ggagccggcg
    23161 ctgccgcttc ctgtttcgca ggagctgccg caggcgctgc gccttcaact tcgaagatca
    23221 taatcagcga gccagttttc actttatcgc caacgttgac tttcagttcc ttcacgacgc
    23281 ctgcaaacgg agccggaact tccatagaag ctttgtcgcc ttctacggta atcagtgact
    23341 gttcagcggc aactttgtcg cccactttca ccatcacttc ggtcacttca acttcgtcac
    23401 cgccgatatc cggaacgtta acttctttca cgccagccgc tggtgcaggg gccgctgccg
    23461 gagccgcttc ctgtttagcc gccggagctg ccgcgcctgc ttcacccgcg acttcgaaga
    23521 ccataatcag cgagccggta gacactttgt cacccacgtt cactttgatc tctttcacgg
    23581 tgccagcaaa cggagccgga acttccatag acgctttatc gccttctacg gtgatcagcg
    23641 actgttcagc ttcaacttta tcgccaactt tcaccaggat ttcggtcact tcaacttcgt
    23701 cgctgccgat atccggaacg ttaacgtctt ttgccgctgc agccgctggt gctgctgccg
    23761 gagctgcttc tttcttctct tctgcctgag caggtgcagc gtctgctgca ccgtcggcgg
    23821 aatcgaaaat cataatcagt gcgccggtct gggttttatc gccaacagag actttgatct
    23881 ctttaacgat acccgcctgc ggagacggaa cttccataga ggctttgtcg ccttctacgg
    23941 tgatcagcga ctgttcggct tcaactttgt cgcccacttt gaccaggatc tcggtgattt
    24001 caacttcatc agccccgatg tccggtactt tgatttcgat agccattatt cttttacctc
    24061 ttacgccaga cgcgggttaa ctttatctgc atcgatgttg aatttggcga ttgcgtcagc
    24121 aaccactttc ttatcgattt cgccacgttt agccagttcg cccagcgccg ctacaaccac
    24181 gtaggaagca tcaacttcga agtggtgacg caggttctca cggctgtcgg aacgaccgaa
    24241 gccatcagta cccagtacgc ggtagtcgtc agccggtacg taagtacgga cctgctcagc
    24301 gaacagtttc atatagtcgg tagatgccac tgccggagcg tcgttcatca cctgagcgat
    24361 atacggaacg cgcggagttt ccagcgggtg cagcatgttc cagcgttcac aatcctgacc
    24421 atctcgcgcc agttcggtga aggaggtcac gctataaacg tcagaaccta cgccgtaatc
    24481 tttcgccagg atctctgccg cttcacggac gtgacgcagg atggaaccgg agcccagcag
    24541 ctgaacttta cctttgctac cttcaatggt ttcgagtttg tagatacctt tacggatacc
    24601 ttcctcagca ccttccggca ttgccggcat gtggtagttt tcgttcagcg tagtgatgta
    24661 gtagtaaacg ttctcttgtt tttcaccgta catacgctcc agaccgtcat gcataatgac
    24721 agcaacttcg taagcgtaag ccgggtcgta agagatacag ttcgggatag tcagcgactg
    24781 aatatggctg tgaccatctt cgtgctgcag accttcaccg ttcagggtgg tacgaccgga
    24841 agtaccgccg atcaggaagc cacgcgcttg ctggtcgcca gccgcccagc acagatcgcc
    24901 gatacgctgg aagccgaaca tcgagtaata gatgtagaac ggaatcatcg gcagattgtt
    24961 ggtgctgtaa gaggtcgccg ctgccagcca ggaacaacct gcgcccaact cgttgatccc
    25021 ttcctgcaga atctgacctt tctcgtcttc tttatagtaa gcaacctgct cgcggtcctg
    25081 cggggtgtac tgctgaccgt tcgggctgta aataccaatc tgacggaaca gaccttccat
    25141 accgaaagta cgcgcttcgt cggcgatgat cggtaccaga cgatctttga tcgacttgtt
    25201 cttcagcatt acgttcaggg cacgaacgaa agcgatagtg gtagagatct ctttgctctg
    25261 ctcttccagc agcgcgccga agtcttgcag gctcggcagc tcaagcttct cggtgaagtt
    25321 cggctgacgg cttggcagat aaccgtgcag tttctgacgc tgtgcgtgca gataggtatg
    25381 ctcttcagaa ccttccggga aggtgatgta cggcagtttt tcgatatctg catcagacac
    25441 cggcacattg aaacggtcgc ggatgtgacg cacgccgtcc atgttcattt tcttaacctg
    25501 gtgcgcgatg tttttacctt cagccgcgtc gcccatgccg taacctttaa tggtatgagc
    25561 aaggattact gtcgctttgc ctttggtttc ctgcgctttc ttgaatgcag cgtagatttt
    25621 cttcggatcg tgaccaccac ggttcagtgc ccagatctgc tcgtcagtcc agtctgcaac
    25681 cagtgctgcg gtttcaggat atttaccgaa gaagtgttca cgaacgtacg caccatcttt
    25741 cgatttgaag gtctggtagt cgccgtcaac ggtttcgttc atcagctgga tcagtttacc
    25801 gctggtatct ttacgcagca gttcatccca acggctaccc cacatcactt tgatcacgtt
    25861 ccagccagca ccttcgaaga tgccttccag ttcgttgatg atcttgccgt taccggtgac
    25921 cgggccgtca agacgctgca ggttacagtt gataacgaag accaggttat ccagtttttc
    25981 acgggtagcg atggtgatcg cacctttgga ttccggttcg tccatttcac cgtcgccgag
    26041 gaacgcgtaa acggtctgtt tagaggtatc tttcaggcca cggtgttcca gatatttcag
    26101 gaatttagcc tggtaaatag caccaatcgg ccccagaccc atagatacgg tcgggaactg
    26161 ccagaattcc ggcatcagtt tcgggtgcgg ataggaagag aggccattgc cgtgaacttc
    26221 ctgacggaag ttatccagct gctcctgagt cagacgacct tccaggaaag cacgagcgta
    26281 cacgcccggg gagatgtggc cctggaagta aaccaggtcg ccgccatcct gctcgttgcg
    26341 tgcacggaag aagtggttaa agcacacatc ataaatggtt gcggaagact ggaaggacgc
    26401 catatggccg cccagttcga ggtctttttt cgacgcacgc agcaccgtca tgatggcgtt
    26461 ccagcggata gctgaacgaa tacggcgttc cagttccaga ttacccggat actccggttg
    26521 ttcttcaacg gggatggtgt tgatgtagtt gctgatacct gtgcctgcgg ctacgtttac
    26581 accgcctttg cgggcttcag caagcagttg gtcgatcaga tactgagcac gctcaacacc
    26641 ttcttcacgg atgaccgatt cgatcgcctg gagccagtcg cgagtttcga tcggatccac
    26701 gtcatttggg aaacgttctg acatgggtta ttccttatct atctaataac gttgagtttt
    26761 ctggaacctg tcccattgaa ctctcgccgg aaagctcaat aagacaggtt ctacgtttag
    26821 ttgccgcgct tttatatgcg cttgatttac aacatcttct ggataatttt taccagaaaa
    26881 atcactaatt ctttcgttgc tccagacgac gcagagaacg ctcacggcgg ctctcttcac
    26941 gacttctgtc gagcaaaatt tcttcgataa aggccagatg gcgatgcgat gcttcgcgcg
    27001 cttcttccgg cttaccggcc ataatcgctt caaatatgcg ggtgcggtga ctactcacca
    27061 gcggcagcat ctcgcgacgc gaatagagca attcgaagtt ctggcggaca ttctgggcca
    27121 acatcggctc catacacctt agcagatgaa gcagaaccac attgtgggcc gcttcggtga
    27181 cggcaatctg atactggagt acggcgtttg attccgcgtc cagatcgcca gactgctgcg
    27241 ccagctctat ggcgtggtgg agttcacgga tgcgttcctt gtcttcatcg gtactacgca
    27301 gcgcggcgta ataagcggcg ataccttcca gggcgtgtcg tgtttcgagc aagtcatact
    27361 gtgactcagg atggtcggag agcagctcca ccagcggatc gctgaagctt tgccataggc
    27421 tgctctggac aaaagtgccg ccaccctggc gacgaagcaa caagcccttc gcttcgagac
    27481 gttgaatcgc ctcacgcaag gagggacggg agacgtcaaa ctgttttgcc agttcgcgtt
    27541 ccggtgggag tttttcgccc gggcggagag tgccttcgag gatcaaaaac tccagttgct
    27601 gctcaatcac atcggagagt tttggttggc ggattttgct gtaggccatg agttcctgtc
    27661 ttaagccact tgccgaagtc aattggtctt accaatttca tgtctgtgac gctaaagtaa
    27721 caaagtattc accttatgtc catacaggtt ttgattgaaa tcatgaaact gtgcacattt
    27781 taacaacttg acatatataa cgtttcaaag ttgtaactat gcacaa
//
LOCUS       DAFNBJ010000056        27354 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000056 DAFNBJ010000000
VERSION     DAFNBJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.056"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            342..2765
                     /gene="pgaA"
                     /locus_tag="L2X49_002212"
     CDS             342..2765
                     /gene="pgaA"
                     /locus_tag="L2X49_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HBN4797294.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2774..4792
                     /gene="pgaB"
                     /locus_tag="L2X49_002213"
     CDS             2774..4792
                     /gene="pgaB"
                     /locus_tag="L2X49_002213"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HBN4797295.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4785..6110
                     /gene="pgaC"
                     /locus_tag="L2X49_002214"
     CDS             4785..6110
                     /gene="pgaC"
                     /locus_tag="L2X49_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HBN4797296.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6112..6525
                     /gene="pgaD"
                     /locus_tag="L2X49_002215"
     CDS             6112..6525
                     /gene="pgaD"
                     /locus_tag="L2X49_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HBN4797297.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6575..7363)
                     /gene="phoH"
                     /locus_tag="L2X49_002216"
     CDS             complement(6575..7363)
                     /gene="phoH"
                     /locus_tag="L2X49_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBN4797298.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7982..9253)
                     /gene="efeB"
                     /locus_tag="L2X49_002217"
     CDS             complement(7982..9253)
                     /gene="efeB"
                     /locus_tag="L2X49_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBN4797299.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9259..10386)
                     /gene="efeO"
                     /locus_tag="L2X49_002218"
     CDS             complement(9259..10386)
                     /gene="efeO"
                     /locus_tag="L2X49_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBN4797300.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10444..11274)
                     /locus_tag="L2X49_002219"
     CDS             complement(10444..11274)
                     /locus_tag="L2X49_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBN4797301.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            11310..11612
                     /locus_tag="L2X49_002220"
     CDS             11310..11612
                     /locus_tag="L2X49_002220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797302.1"
                     /translation="MQRKVNRSDSDYHYGKEKYKRNVYKMMLECKQREVMPRDINDYS
                     RVERWDCLQRTMCSCRMPRKRRIQPTKLSRLNASELPCKTDKRVYQAISSIRDYQI"
     gene            complement(11817..13325)
                     /gene="putP"
                     /locus_tag="L2X49_002221"
     CDS             complement(11817..13325)
                     /gene="putP"
                     /locus_tag="L2X49_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HBN4797303.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GVYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            13747..17709
                     /gene="putA"
                     /locus_tag="L2X49_002222"
     CDS             13747..17709
                     /gene="putA"
                     /locus_tag="L2X49_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HBN4797304.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRIMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17749..18387)
                     /gene="rutR"
                     /locus_tag="L2X49_002223"
     CDS             complement(17749..18387)
                     /gene="rutR"
                     /locus_tag="L2X49_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBN4797305.1"
                     /translation="MTQGAVKTTGKRSRTVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            18675..19766
                     /gene="rutA"
                     /locus_tag="L2X49_002224"
     CDS             18675..19766
                     /gene="rutA"
                     /locus_tag="L2X49_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBN4797306.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQALRDLWGSGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19766..20458
                     /gene="rutB"
                     /locus_tag="L2X49_002225"
     CDS             19766..20458
                     /gene="rutB"
                     /locus_tag="L2X49_002225"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBN4797307.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            20470..20856
                     /gene="rutC"
                     /locus_tag="L2X49_002226"
     CDS             20470..20856
                     /gene="rutC"
                     /locus_tag="L2X49_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBN4797308.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRTVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20864..21664
                     /gene="rutD"
                     /locus_tag="L2X49_002227"
     CDS             20864..21664
                     /gene="rutD"
                     /locus_tag="L2X49_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBN4797309.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHAVRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21674..22264
                     /locus_tag="L2X49_002228"
     CDS             21674..22264
                     /locus_tag="L2X49_002228"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBN4797310.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDNSKLYARLPRLSFEEACGLL"
     gene            22275..22769
                     /gene="rutF"
                     /locus_tag="L2X49_002229"
     CDS             22275..22769
                     /gene="rutF"
                     /locus_tag="L2X49_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBN4797311.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22790..24118
                     /gene="rutG"
                     /locus_tag="L2X49_002230"
     CDS             22790..24118
                     /gene="rutG"
                     /locus_tag="L2X49_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBN4797312.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGVGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(24201..24374)
                     /locus_tag="L2X49_002231"
     CDS             complement(24201..24374)
                     /locus_tag="L2X49_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBN4797313.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24747..25343
                     /gene="wrbA"
                     /locus_tag="L2X49_002232"
     CDS             24747..25343
                     /gene="wrbA"
                     /locus_tag="L2X49_002232"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBN4797314.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25364..25591
                     /locus_tag="L2X49_002233"
     CDS             25364..25591
                     /locus_tag="L2X49_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBN4797315.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25629..26870)
                     /gene="agp"
                     /locus_tag="L2X49_002234"
     CDS             complement(25629..26870)
                     /gene="agp"
                     /locus_tag="L2X49_002234"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBN4797316.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(27162..>27354)
                     /locus_tag="L2X49_002235"
     CDS             complement(27162..>27354)
                     /locus_tag="L2X49_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797317.1"
                     /translation="NVASISLGNESFSTDDDLEYGYLINTGNHYDVYLPPELFAQAYE
                     LNNKERNAQIDFLTRYAIY"
BASE COUNT         6688 a         7064 c         6934 g         6668 t
ORIGIN      
        1 atccgtgagt gccgtagcgc agcctttcat caggactttc gaaaaatccg aaatcatgca
       61 tcggaattta ctgatttaat tattttaatc ctaatttatt ttgaaaaagg cattgggatt
      121 tatgccgtat tcctgaagat cctcatcatt ggaatggatt ttcgggcgag aaaaggattt
      181 tatatggaca ctctgctcat catttcttct tctcatcatc aacaattcac gtctctcttc
      241 cgcgtttaat aacggattat gaggtgcaaa aatatctttc ttttcagtta cctgtaatta
      301 gatacagaga gagattttgg cgatacatgg agtaatacag gatgtattca agtagcagaa
      361 aaaggtgccc gaaaaccaaa tgggctttga aaattcttac tgccgcattt ttagcagcga
      421 gtcccgcggc gaagagtgcc gttaataacg cctatgatgc attgattatt gaagctcgca
      481 agggaaatac tcagccagct ttgttatggt ttgcacaaaa atcagcactc agtaataacc
      541 aaattgctga ctggttacag attgcattat gggccgggca agataaacag gttattaccg
      601 tttacaaccg ctaccgttat cagcaattac cagcgcgtgg ttatgcagct gtcgccgtcg
      661 cttatcgtaa cctgcaacaa tggcaaaact cgcttacact gtggcaaaag gcgctctctc
      721 tggagccgca aaataaggat tatcaacggg gacaaatttt aaccctggca gatgcgggtc
      781 actatgatac tgcgctggtt aaacttaagc agcttaactc tggagcaccg gacaaagcca
      841 atttactcgc agaagcctat atctataaac tggcggggcg tcatcaggat gaattacggg
      901 cgatgacaga gtcattacct gaaaatgcat ctacgcaaca atatcccaca gaatacgtgc
      961 aggcattacg taataatcaa cttgctgccg cgattgacga tgccaattta acgccagata
     1021 ttcgcgctga tattcatgcc gaactggtca gactgtcgtt tatgcctacg cgcagtgaaa
     1081 gtgaacgtta tgccattgcc gatcgcgccc tcgcccaata cgctgcatta gaaattctgt
     1141 ggcacgataa cccagaccgc actgcccagt accagcgtat tcaggttgat catcttggcg
     1201 cgttattaac tcgcgatcgt tataaagacg ttatttctca ctatcagcga ttaaaaaaga
     1261 cggggcaaat tattccgccc tgggggcaat attgggttgc atcggcttat ctcaaagatc
     1321 atcagccgaa aaaagcacag tcaataatga ccgagctctt ttatcacaag gagaccattg
     1381 ccccggattt atccgatgaa gaacttgcgg atctctttta cagccacctg gagagtgaaa
     1441 attatccggg cgcgctaact gtcacccaac ataccattaa tacttcgccg cctttccttc
     1501 ggttaatggg cacgcctacg agcatcccga atgatacctg gttacagggg cattcgtttc
     1561 tctcaaccgt agcaaaatat agtaatgatc ttcctcaggc tgaaatgaca gccagagagc
     1621 ttgcttataa cgcacctgga aatcagggac tgcgcattga ttacgcgagt gtgttacaag
     1681 cccgcggttg gcctcgtgca gcagaaaatg aattaaaaaa agcagaagtg atcgagccac
     1741 gtaatattaa tctggaggtt gaacaagcct ggacagcatt aacgttacaa gaatggcagc
     1801 aggcagctgt cttaacgcac gatgttgtcg aacgtgaacc gcaagatccc ggcgttgtac
     1861 gtttaaaacg tgcggttgat gtacataatc ttgcagagct tcgtatagct ggttcaacag
     1921 gaattgatgc cgaaggcccg gatagtggta aacatgatgt cgacttaacc accatcgttt
     1981 attcaccacc gctgaaggat aactggcgcg gttttgctgg attcggttat gccgatggac
     2041 aatttagcga aggaaaaggg attgttcgcg actggcttgc gggtgttgag tggcggtcac
     2101 gtaatatctg gctcgaggca gagtacgctg aacgcgtttt caatcatgag cataaacccg
     2161 gcgcgcgcct gtctggctgg tatgatttta atgataactg gcgtattggt tcgcaactgg
     2221 aacgcctctc tcaccgcgtt ccattacggg caatgaaaaa tggtgttaca ggcaacagtg
     2281 ctcaggctta tgttcgctgg tatcaaaatg agcggcgtaa gtacggtgtc tcctgggctt
     2341 tcactgattt ttccgacagt aaccagcgtc atgaagtctc acttgagggt caggaacgca
     2401 tctggtcttc accatatttg attgtcgatt tcctacccag tctgtattac gaacaaaata
     2461 cagaacacga taccccatac tacaacccta taaaaacgtt cgatattgtt ccggcatttg
     2521 aggcaagcca tttgttatgg cgaagctatg aaaatagctg ggagcaaata ttcagcgcag
     2581 gtgttggtgc ctcctggcaa aaacattatg gcacggatgt cgtcacccaa ctcggctacg
     2641 ggcaacgcat tagttggaat gacgtgattg atgctggcgc aacgctacgc tgggaaaaac
     2701 gaccttatga cggtgacaga gaacacaact tatacgttga attcgatatg acattcagat
     2761 tttaaggata aatatgttac gtaatggaaa taaatatctc ctgatgctgg tgagtataat
     2821 tatgctcacc gcgtgcatta gccagtcaag aacatcattt ataccgccac aggatcgcga
     2881 atctttactc gccgagcaac cgtggccgca taatggtttt gtagcgattt catggcataa
     2941 cgttgaagac gaagctgccg accagcgttt tatgtcagtg cggacatcag cactgcgtga
     3001 acaatttgcc tggctgcgcg agaacggtta tcaaccggtc agtattgctc aaattcgtga
     3061 agcacatcga ggaggaaaac cgctaccgga aaaagctgta gtgctgactt ttgatgacgg
     3121 ctaccagagt ttttataccc gcgtcttccc aattcttcag gccttccagt ggcctgctgt
     3181 atgggccccc gtcggcagtt gggtcgatac gccagcggat aaacaagtaa aatttggcga
     3241 tgagttggtc gatcgagaat attttgccac gtggcaacaa gtgcgagaag ttgcgcgttc
     3301 ccggctcgtt gagctcgctt ctcatacatg gaattctcac tacggtattc aggctaatgc
     3361 caccggcagc ttattgcctg tatatgtaaa tcgtgcatat tttactgacc acgcacggta
     3421 tgaaaccgca gcagaatacc gggaaagaat tcgtctggat gctgtaaaaa tgacggaata
     3481 cctgcgtaca aaggttgagg taaatccaca cgtttttgtt tggccttatg gcgaagcgaa
     3541 tggcatagcg atagaggaat taaaaaaact cggttatgac atgttcttca cccttgaatc
     3601 aggtttggca aatgcgtcgc aattggattc cattccgcgg gtattaatcg ccaataatcc
     3661 ctcattaaaa gagtttgccc agcaaattat taccgtacag gaaaaatcac cacaacggat
     3721 aatgcatatc gatcttgatt acgtttatga cgaaaacctc cagcaaatgg atcgcaatat
     3781 tgatgtgcta attcagcggg tgaaagatat gcaaatatca accgtgtatt tgcaggcatt
     3841 tgctgatccc gatggtgatg ggctggtcaa agaggtctgg tttccaaatc gtttgctacc
     3901 aatgaaagca gatattttta gtcgggttgc ctggcaatta cgtacccgct caggtgtaaa
     3961 catctatgcg tggatgccgg tattaagctg ggatttagat cccacattaa cgcgagtaaa
     4021 atacttacca acaggggaga aaaaagcaca aattcatcct gaacaatatc accgtctctc
     4081 tcctttcgat gacagagtca gagcacaagt tggcatgtta tatgaagatc ttgccggaca
     4141 tgctgctttt gatggcatat tgttccacga tgatgctttg ctttcagatt atgaagatgc
     4201 cagtgcaccg gctatcacgg cttatcagca agcaggcttt agcgggagtc tgagcgaaat
     4261 tcgacaaaac ccggagcaat ttaaacagtg ggcccgcttt aaaagtcgtg cgttaactga
     4321 cttcacttta gaacttagtg cgcgcgtaaa agccattcgc ggtccacata ttaaaactgc
     4381 acgaaatatt tttgcacttc cggtaataca acctgaaagt gaagcctggt ttgcacagaa
     4441 ttatgctgat ttcctaaaaa gctatgactg gaccgctatt atggctatgc cttatctgga
     4501 aggtgtcgca gaaaaatcgg ctgaccaatg gttaatacaa ttgaccaatc aaattaaaaa
     4561 catccctcag gctaaagaca aatctatttt agaattacag gcacaaaact ggcagaaaaa
     4621 tggtcagcat caggctattt cttcgcaaca actcgctcac tggatgagcc tattacaact
     4681 gaatggagtg aaaaactatg gttattatcc cgacaatttt ctgcataacc aacctgaaat
     4741 agaccttatt cgtcctgagt tttcaacagc ctggtatccg aaaaatgatt aatcgcatcg
     4801 tatcgttttt tatattatgt ctggtgttat gcatacccct atgcgtagcg tactttcact
     4861 ctggtgaact gatgatgagg ttcgttttct tctggccgtt ttttatgtcc attatgtgga
     4921 ttgttggcgg cgtctatttc tgggtctatc gtgaacgcca ctggccgtgg ggagaaaacg
     4981 caccagctcc ccagttgaaa gataatccgt ctatctccat tatcattccc tgttttaatg
     5041 aggagaaaaa cgttgaggaa accatacacg ccgctttagc acagcgttat gagaacattg
     5101 aagttattgc cgtaaatgac ggttcaacag ataaaacccg tgccatcctg gatcgcatgg
     5161 ctgcacaaat tccccatttg cgggtcattc atctggcgca aaaccagggg aaagccattg
     5221 cgcttaaaac cggagctgcc gcggcgaaaa gtgaatatct ggtgtgcatt gatggcgatg
     5281 cgttattaga ccgcgatgcg gcggcatata ttgtggaacc gatgttgtac aacccgcgtg
     5341 tgggtgccgt aaccggtaat cctcgtattc gaacacgttc taccctggtg ggtaaaattc
     5401 aggttggcga gtattcctca attattggtt tgatcaagcg aacccagcgt atctatggaa
     5461 acgtatttac cgtttccggt gttattgccg catttcgtcg cagcgccctg gcagaagtgg
     5521 gttactggag tgacgatatg atcaccgaag atattgatat tagctggaag ctgcagttga
     5581 atcagtggac gattttttac gagccacggg cactgtgctg gatattaatg cctgaaacgt
     5641 taaaagggct gtggaaacag cgcctgcgct gggctcaggg cggtgcagaa gtattcctca
     5701 aaaatatgac aaggttgtgg cgcaaagaaa actttcgaat gtggccgctg ttttttgaat
     5761 actgcctgac gacaatatgg gccttcacct gcctggtcgg tttcattatt tacgcagtcc
     5821 aacttgccgg tgtaccgtta aatattgaat tgacacatat cgctgcgaca catactgccg
     5881 gaatattatt gtgtacgtta tgtttactgc aatttattgt cagcctgatg atcgagaatc
     5941 gctatgagca taatctgact tcatcgcttt tctggattat ttggttcccg gttattttct
     6001 ggatgctgag cctggcaacg acattggtat catttacacg agtcatgttg atgcctaaaa
     6061 agcaacgcgc ccgttgggta agtcccgatc gcgggattct gagaggttaa tatgaacaat
     6121 ttaattatta cgacccgaca atcaccagta cgtttactgg ttgattatgt tgccacaacc
     6181 atcttgtgga cattatttgc gttgttcata ttcttattcg ccatggatct gctgacgggt
     6241 tattactggc aaagcgaggc cagaagccga cttcagttct attttttgct ggcagtggcg
     6301 aatgccgtcg tgttaattgt ctgggcgctg tacaataagc tgcgttttca aaaacagcag
     6361 catcatgcag cctaccaata tacgccgcaa gaatatgcag agagcttagc aatacctgat
     6421 gagctctatc agcaactaca aaaaagccac aggatgagcg tacacttcac cagccagggg
     6481 caaataaaaa tggttgtttc agaaaaagcg ctagtccggg cataaacacc caaaacaaag
     6541 cccggttcgc ccgggctctg caccgataac acacttaact gtaggcatgc agcgtacgtt
     6601 ggcaaagtgc cgaacgtacg cagtcctctt taccgaaccg gacgatccca accatttcat
     6661 cttcttcgaa acgttccagc gcgtcactta atccggagca cacgccgcga ggcaaatcgc
     6721 attgcgtgat atcaccgttg acgataaccg tcacgttctc cccgaggcgg gttaaaaaca
     6781 ttttcatttg cgcggcagtc acattctgcg cctcgtcaag aatgacgact gcattttcaa
     6841 aggtacgtcc acgcatatag gcgaacggcg caatttccac cttccctatt tccggtcgca
     6901 ggcagtactg cataaaggaa gcccctaagc gccggaccag cacgtcgtag accgggcgaa
     6961 aatagggagc aaacttttct gcgatatctc caggtaagaa gccaagatct tcatcggctt
     7021 gcagaactgg acgggtgacg ataatcctgt cgacatcctt atgtatcagg gcctctgccg
     7081 cttttgctgc gctgatccag gtttttccgc acccggcttc gcccgtggcg aatatcagct
     7141 gcttactctc aatagccttc agatagtgca attgcgcttc atttcgcgcg aggatgggcg
     7201 aagtatcgcg actgtcgcgg gccataccaa tggcttctac gccgcccatc tgcacaagcg
     7261 aggtgaccga ttcttcttca cgctgcttat ggctgcgcga atcccgtctc agcacacgtt
     7321 ttgcctcacg acgagctttg atcactgctt tttgtcttcc catggagagc accttgagtt
     7381 gtttgtattc atcacacgcg ccgttggcag cgcgattatg cgcacgaaca tcagagggtt
     7441 ggcttccttg taagccatag tttgcttttg gataaaatgc cgaaaaacgg ctacgcacac
     7501 cgtttgcggc gtcggtaaca cgagtaaaga gaggaagtga gaaaaataca gagtgacgaa
     7561 gttgctaccg gaggagaaat ctccgcaagt ggtggagcgt agcttaacaa gacattgtcc
     7621 agaacaggac gttaccatcc gcgatctcca tagtgactga ctatcactgc cgggaacttc
     7681 cgctgctact taataattac aacagatctc gcattcattg caacaatata ttcacttata
     7741 tttaaccata aaacaccatt tcagtgacat tagtttctac tggaaagatg acagagtgat
     7801 gacagatatg aaaaaagctg tgtgctttca gcaggattcg catcaggcat ctgcgcacga
     7861 acgccggatg cggcgtgaac gccttatccg cctaccgaac ccgcaccgat tgtaggcata
     7921 agaagacgcg caagcatcgc atcaggcatc tgcgcaagaa cgccttatcc gtctataaac
     7981 attaaacccg caataacgcg cttccgagat aatcgttcgc gtccttcact cccggcagcg
     8041 caaaaaaata accgccgccg ataggtttaa cgtattcctc cagcgcttcg ccattgagcc
     8101 ttttttgtac agtcaggaag cctttttcca gatcgtgttg gtaacagaca aacagtagcc
     8161 ccatatccag ttgcccggag ttggtgacgc ccagtgaata actgtagcca cgacgcagca
     8221 tcaggctgga ctcactctcc gccgtgcggg gattcgccag ccggatatgg ctgtccagcg
     8281 caatcacctt cccttccgga tcgctggcgt aatcgggaac atcatgctca tgctgcattc
     8341 ccagcggcgc accggtttgc ttatcacgac caaaaatcgt ctgctgttct ttcagcggag
     8401 ttctgtccca aaattccact cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga
     8461 ttgtccacgc tggctcctgc tgatctgccg ttacccacac cactttttgc atcaacttat
     8521 cattctggct atcgggattg gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct
     8581 ctttgccttt actacgcgcc gcgtgatcgg aaataaaccc ttcccgcttc cagcgcacgc
     8641 tgagcaaatc tggcgtatgt ttgatgatat cgcgcagcgc atggatcacc gtgtcctggg
     8701 tgttggcgca aatttgtagc aacacatcac catgacataa cgccgcatcc agcgagtcgt
     8761 tgggaaaacg cgtcatcttc tgcaactttt tcggcatctg tggcgcaagg ccaaagcgct
     8821 catcaaacaa tgagtgaccc accgataacg tgatggtgag attatcgggc gcaatgtagc
     8881 cgccaagaat gccggaatcg agtggtggca ggcgcggatt tggcgtttct ggtgccgctc
     8941 cgccctgagt cagaaaagca aaacgctgag tcaacaagcg aaacaaccgt tcaagatcgg
     9001 gtttatcgct ggcaagcaca tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca
     9061 ggatccctgc ctgatgctca ccataaaacg gctgtttctc attgcgtgca tccggtgaaa
     9121 gcgtacccgg cgcactttgc gttttttgtg catgagcgac cggacaactt cccgccagcg
     9181 ccagcgcacc aatgcctttc agtaaacgtc ggcgtgacgg ttcattcacg ccgtttttat
     9241 cttcatactg cataatgctt aatccaatcc cagcacaccg cgaagttgcg ccagatcttc
     9301 cgccagcgca gtaatcggtc ctttcagtgc attccggtcg gcatcggtca atttgtcgta
     9361 ggtttcaaaa ccgtctttag tacggtattt cgccagaatg gtatcgacct ttttaaagtt
     9421 ggcatcgact tttgccaaca gttccgggtt ggctttttgc agttgtggac gcagcagatc
     9481 gacaattttc tgcgagcctt caacgttagc ctggaaatcc cacagatcgg tgtggctgta
     9541 gcgatcttct tcaccgctga ttttgctggc tgccacttcc tcaatcagtc cggctgcacc
     9601 gccgaccact tttgaaggtg ggaaagccag ttcactgatg cgtttttgca aatcgaccac
     9661 atcggtataa agctggtcag cgtactgatc catgcctttg gtggtgttgt cgccaaacaa
     9721 tgctttttcc agacggtgga aaccggtgaa tttcggatcg gcggcttttt gctcgtaatc
     9781 atcttcacgg gcgtcaatgc tgccatccag atcggagaac agttcagcaa tcggttcgat
     9841 gcgctcatag tgctggcgcg tcggcgcata cagtgctttc gctttttcga tatcgcctgc
     9901 tttaatagcg tcagtaaagg ctttcgtgtc ggtcaccagc tgcgtggttt ccgccatgac
     9961 atacgcttta tatgcagtaa ttgcaccacc aagacttaac agcgcatcac tttgcgccgc
    10021 atccgccgtt gcctcacctt tgacgatcaa cttccctttc gggttagtca gcagaccgca
    10081 ggtcatatcg tattcgccag gctgtaaatt cgccgtcatt ttctggctaa agccaggggc
    10141 gatattttcc cgctcttcca ccaccatcac gcctttgagg atctcccact ccagcgcctt
    10201 ctggctgtgg ttctgaataa tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg
    10261 ttcgcactgc ttatcggtca cggtcacctt gacctgcggc acattagcgg cgttagccat
    10321 aaaagcagaa gagaacagcg cagccacgct caactgcaat gcgttacggc ggaagttaat
    10381 ggtcatgacg agactatccc tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat
    10441 ttgctacgcg gagcgagacg ctgtcgcccc tgcgcgtggt ggcagggcaa atgccaccag
    10501 cgccgggatg agataaataa accagacggc gacttcgctg acgctcggtg cttcctgata
    10561 gccaaaaatc ccttccatca gcgtgccaaa cagcgagtga gttgagagga ccgcgctcat
    10621 atcgaaggcg atttcctgaa agtggttcca caatccggct tcatgaaatg cgcgaatggc
    10681 accagccgcc agccctgcgg cgacgaagag aataaacagg ctggtccatt taaaaaatgc
    10741 accaagattg aggcgaatac cgccccagta gagcaggaag ccgagcacca cggcagtagc
    10801 aagaccgagc attgcaccca gcggcggcca gatcccgaca tcttgttgaa atgccgccag
    10861 caggaaaaag accgactcca gcccttccct tgcaacggca aaaaagacca tcatcaccag
    10921 cgcccagcca tgatgatttc cacgctgcaa tgcgctatcg actgcctgtt ccagttgcac
    10981 tttgacgttg cgcgacactt tgcgcatcca gaaaaccatc caggtaagga tcaccacggc
    11041 gatcaccgcc acgatacctt caaacagttc ctgttctttt tgcggaaatt cgccggtggt
    11101 ttcgttaatg aagataccca ggcccaggca caacgcagcg gcaagcaaca cgccaatcca
    11161 catcacacca atccatcggc ctcgctgggt acgcttaaga tagctggcaa tcaaactgac
    11221 aatcagcgcg gcttcaagtc cttcgcgcaa cataatgaga aacggaacaa acatgccacc
    11281 cacccttaaa cgtttttctg agtcaatcta tgcaaagaaa agtaaatcgc agtgatagcg
    11341 attatcatta cggcaaagaa aaatacaagc ggaatgttta taaaatgatg ttggaatgta
    11401 aacaaagaga ggtaatgcca agggatataa acgattattc cagggtagaa agatgggatt
    11461 gtttgcaaag aaccatgtgt tcttgccgga tgccgcgtaa acgccgtatc cagccgacaa
    11521 aactgtccag actcaatgca tcagagcttc catgtaaaac tgataagcgg gtgtatcaag
    11581 caatttcgtc catccgcgat tatcaaatct aactttttgc ccggaacata ataacagctc
    11641 gggtgttggt ggcgtcaaaa tcgctgaagc gttccccgaa ggccgggaca gcagggggcg
    11701 gcggcgattg gccgccccct gcgcgctccc tgcaccattc aatttatatg gcgaggcaca
    11761 ttaagggagc gcaacccgct gctgaagcca aacaaaaccg ccgcaggcta agtcccttag
    11821 ctttcctgca accgtgacgg cggagccgaa tgatagtgcg catcggcctc ggcaaagcgt
    11881 ttttgcatcg ccgctgacgg cgctttaccc agcaaactaa acactacaat cccaatactg
    11941 ccgaagataa agcccggaat aatttcgtag actcccagcc agccgaactg cttccagacg
    12001 ataaccgtca gcgcaccgat gatcatcccc gccagcgcac cgttgcgcgt catgcgtgac
    12061 cacatcaccg agaacagcac caccggacca aacgccgcgc caaagcctgc ccacgcgtag
    12121 ctcactaagc ccagcacgcg gttttccggg tttgccgcca gcgcaatcgc caccagcgcc
    12181 accaccagca ccatcacacg ccctacccac accagctctt tctggctggc atgtttacgc
    12241 agaaacgctt tgtataaatc ttcggtaatc gcactggagc acaccagcag ctggcagctt
    12301 aacgttgaca ttaccgccgc cagaattgcc gacagcagaa tcccggcaat ccacgggtta
    12361 aacagaattt gcgccagttc gataaacaca cgttcagcgt tctggtttac cgcacctgct
    12421 accgccgggt gctcattaaa gtaagcaatc ccaaagaagc cgacagccac cgcccctgcc
    12481 aggcagagga tcatccaggt catactgata cgacgcgcat ggacaatgct gtggtgagaa
    12541 tccgccgcca taaaacgcgc cagaatatga ggctgaccga agtaacccag cccccaaccc
    12601 atcaatgaga taatggcaac aaagttcagc cctttgagca tatccacgtt ttcgatgctc
    12661 ttttgtttga tcacttccag cgagtcacca aagccaccga cactgataat gacgataacc
    12721 ggcgtcagga tcagggcaaa aatcatcagg ctggcctgta cagtgtcagt ccagctcacc
    12781 gcgaggaaac cgccaataaa ggtgtaaagg atcgtcgccg cagcgcccgc ccacagagcc
    12841 gtttcgtagc tcatgccaaa ggtactttca aacagacgcg cgcctgccac aatgcccgaa
    12901 gcgcaataaa tggtgaagaa cagcaaaata accagcgcag agataatgcg caaaatgcgg
    12961 cttttatctt caaagcgccc ggtgaaataa tccggcaatg ttaaggcgtt attgttgtat
    13021 tcggtatgca cacgcaaccg cccggccacc agcttccagt tgatccacgc gcctaatgtc
    13081 aggccaatgg cgatccagct ttcggaaatc ccggaaagaa aaaccgcgcc cggcaacccc
    13141 attaacagcc agccgctcat atccgacgca cccgccgata atgccgtcac gaatggccca
    13201 agactacgac cgcccagaat atagtcgtca aagtttttcg ttgatcgcca agcgataaac
    13261 ccaatcaata tcatgccaaa gatatagaca caaaatgtca ccaacatcgg tgtgctaata
    13321 gccatctaaa gtctccaaaa aattattatc ggcaatgtcg aaacttgccg ttatatctgc
    13381 caccggaacg gggtaacaga gtttatgttt taccagggcg accgtatcct gccggaagcg
    13441 ctggttattc acaatcgatt taacacacca tttacattaa attttagtgc tcagcgacac
    13501 tatttttcat caggttgcac tctctcacat tttttgcggt tgcacctttc aaaaatgtta
    13561 actgccgcag agaaaaagtc tgagttattt tttaatccct gtcatatcga tttcttttat
    13621 taacatttca ttcattttta agcttgctac gcaggtcaca tttaacatgg ttgcacaaag
    13681 ttgcaacatc atggatattt cacgataacg ttaagttgca cctttcagaa caacaggagt
    13741 aatggcatgg gaaccaccac catgggggtt aagctggacg acgcgacgcg tgagcgtatt
    13801 aagtctgccg cgacacgtat cgatcgcaca ccacactggt taattaagca ggcgattttt
    13861 tcttatcttg aacaactgga aaacagcgat actctgccgg agctacctgc gctgctttct
    13921 ggcgcggcca atgagagcga tgaagcaccg actccggcag aggaaccaca ccagccattc
    13981 ctcgactttg ccgagcaaat attgccccag tcggtttccc gcgccgcgat cacagcggcc
    14041 tatcgccgcc cggaaaccga agcggtttct atgctgctgg aacaagcccg cctgccgcag
    14101 ccagttgctg aacaggcgca caaactggcg tatcaactgg ccgataaact gcgtaatcaa
    14161 aaaaatgcca gtggtcgcgc aggcatggtt caggggttat tgcaggaatt ttcgctgtca
    14221 tcgcaggaag gtgtggcgct gatgtgtctg gcagaagcgt tgttgcgtat tcccgacaaa
    14281 gccacccggg atgcattaat tcgcgacaaa atcagcaacg gtaactggca gtcacacatt
    14341 ggtcgtagcc cgtccctgtt tgttaatgcc gctacctggg ggctgctgtt taccggcaaa
    14401 ctggtttcca cccataacga agccagcctc tcccgctcgc tgaaccgcat tatcggtaag
    14461 agcggtgaac cgctgatccg caaaggtgtg gatatggcga tgcgcctgat gggcgagcag
    14521 ttcgtcacgg gtgaaaccat tgcagaagcg ttagccaatg cccgcaagct ggaagagaaa
    14581 ggtttccgtt actcttacga tatgcttggc gaagctgcac tgactgccgc agatgcacag
    14641 gcgtatatgg tttcctatca gcaggcgatt cacgccattg gtaaagcgtc caatggtcgt
    14701 ggcatctatg aagggccggg catttcaatc aaactgtcgg cgctgcatcc gcgttatagc
    14761 cgcgcccagt atgaccggat aatggaagag ctttacccgc gtctgaaatc actcaccctg
    14821 ctggcgcgtc agtacgatat tggtattaac atcgatgccg aagaggccga tcgtctggag
    14881 atctccctcg atctgctgga aaaactctgt ttcgagccgg aactggcagg ctggaacggc
    14941 atcggttttg ttattcaggc ttatcaaaaa cgctgcccgt tggtgatcga ttacctgatt
    15001 gatctcgcca cccgcagccg tcgccgtctg atgattcgcc tggtgaaagg cgcgtactgg
    15061 gatagtgaaa tcaagcgtgc gcagatggac ggccttgaag gttatccggt ttatacccgc
    15121 aaggtgtata ccgacgtttc atatctcgcc tgtgcgaaaa agctgctggc ggtgccgaat
    15181 ctaatctacc cgcagttcgc gacgcacaac gcccatacgc tggcagcgat ttatcaactg
    15241 gcaggtcaaa actactatcc gggtcagtac gagttccagt gcctgcatgg tatgggcgag
    15301 ccactgtatg agcaggtcac cgggaaagtt gccgacggca aacttaaccg tccgtgtcgt
    15361 atttatgctc cggttggcac acatgaaacg ctgctggcgt atctggtgcg tcgcctgctg
    15421 gaaaacggtg ctaacacctc gtttgttaac cgtattgccg acacctcttt gccactggat
    15481 gaactggtcg ccgatccggt cactgctgta gaaaaactgg cgcaacagga agggcaaact
    15541 ggattaccgc atccgaaaat tcccctgccg cgcgatcttt acggtcacgg gcgcgacaac
    15601 tcggcagggc tggatctcgc taacgaacac cggctggcct cgctctcttc tgccctgctc
    15661 aatagtgcac tgcaaaaatg gcaggccttg cccatgctgg aacaaccggt agcggcaggt
    15721 gagatgtcgc ccgttattaa ccctgcggaa ccgaaagata ttgtgggctt tgtgcgtgaa
    15781 gccacgccgc gtgaagtaga acaggcgctg gaaagtgcgg ttaataacgc gccaatctgg
    15841 tttgccactc ctccggttga acgcgcggcg attttgcatc gcgctgccgt gctaatggaa
    15901 agccagatgc agcaactgat tggtattctg gtgcgtgaag ccggaaaaac cttcagtaac
    15961 gccattgccg aagtgcgcga agcggtcgat tttctccact actatgccgg acaggtgcgg
    16021 gatgatttcg ctaacgaaac ccaccgtcca ttagggcctg tggtgtgtat cagtccgtgg
    16081 aacttcccgc tggcgatttt caccgggcag atcgctgccg cactggcggc aggtaacagc
    16141 gtgctggcaa aaccggcaga acaaacgccg ctgattgccg cgcaagggat cgccattttg
    16201 ctggaagcgg gtgtaccccc tggcgtggtg caattgctgc caggtcaggg ggaaaccgtg
    16261 ggcgcgcaac tgacgggtga tgatcgcgtg cgcggggtga tgtttaccgg ttcaaccgaa
    16321 gtcgctacgt tactgcagcg caatatcgcc agccgcctgg acgctcaggg tcgccctatt
    16381 ccgctcatcg ctgaaaccgg cggcatgaac gcgatgattg tcgattcttc agcactgacc
    16441 gaacaagtcg tcgtggatgt gctggcctcg gcgttcgaca gtgcgggtca gcgttgttcg
    16501 gcactgcgcg tgctgtgcct gcaagatgag attgccgacc acacgttgaa aatgctgcgc
    16561 ggtgcaatgg ccgaatgccg gatgggtaat ccgggtcgcc tgaccaccga tatcggtccg
    16621 gtgattgata gcgaagcgaa agccaatatt gagcgccata ttcagaccat gcgtagcaaa
    16681 ggccgtccgg tgttccaggc ggtgcgggaa aacagcgaag atgcccgtga atggcaaagc
    16741 ggcacctttg tcgccccgac gctgatcgaa ctggatgact ttgccgaatt gcaaaaagag
    16801 gtctttggtc cggtgctgca tgtggtgcgt tacaaccgta accagctacc agagctgatc
    16861 gagcagatta acgcttccgg ctatggtctg acgcttggtg tccatacgcg tattgatgaa
    16921 accatcgccc aggtcactgg ctcggcccat gttggtaacc tgtatgttaa ccgtaatatg
    16981 gtgggcgcag tggtgggtgt gcagccattc ggcggcgaag ggttgtccgg taccgggccg
    17041 aaagcaggcg gtccgctcta tctctaccgt ctgctggcga atcgcccgga aagtgcgctg
    17101 gcagtgacgc tcgcgcgtca ggatgcagag tatccggtcg atgcacagtt gaaagccgca
    17161 ttgactcagc cgctaaatgc actgcgggaa tgggcagcaa atcgtccaga attgcaggcg
    17221 ttatgtacgc aatatggcga gctggcgcag gcaggaacac aacgattgct gccggggccg
    17281 acgggtgaac gcaacacctg gacgctgctg ccgcgtgagc gcgtgttgtg tattgccgat
    17341 gatgagcagg atgcgctgac tcagctcgcc gccgtgctgg cggtgggcag ccaggtactg
    17401 tggccggatg acgcgctgca tcgtcagcta gtgaaggcat tgccatcggc agtcagcgaa
    17461 cgtattcaac tggcgaaagc ggaaaatata accgctcaac cgtttgatgc ggtgatcttc
    17521 cacggtgatt cggatcagct tcgcgcattg tgtgaagccg ttgccgcgcg ggatggcgca
    17581 attgtttcgg tgcagggttt tgcccgtggc gaaagcaata tccttctgga acggctgtat
    17641 atcgagcgtt cgctgagtgt gaataccgct gccgctggcg gtaacgccag cttaatgact
    17701 ataggttaaa caggtagccg gaggatgtta caacctcctc cggcatcttt aacgtggtcg
    17761 aatcccctca ataataatcc gctgcacgtt ttcaaccgtt tgattgaaaa atacctcatc
    17821 gcgcaacgtc gcgcctgtca ccgcctccac ctgaggggcg aaatcggcgt aatgttgagt
    17881 ggaagcccaa atcataaaaa tcaaatgctg cggatcaatc ggcgcgagtt tgccgctttt
    17941 gacccaaccg gcaatcagcg ccgatttctc atcaattaat gccttcaaat cgcccgtcag
    18001 ttcatccatt aacaacggcg cgcctgccag catctccata cagaacaggc gcgaagcctg
    18061 cggataatcg cgtgagactt ccagcttcag acggatgtac tctttgatcg ccgccagcgg
    18121 ggcgaaatct tcacgaaacg cttttaacgg tgccagccag atatcgagaa tctgccgcag
    18181 cacggcaata tacagcgcct cttttgacgg aaagtaatac agcagattgg tttttgaaac
    18241 acccgccaac tctgcgatct gctccagcct tgtgccgtga aaaccgaatt gtgaaaaagt
    18301 gtccagcgct gcgctaagaa tcgctttttt cttcgcgctt actgtacgcg aacgtttacc
    18361 cgttgttttg actgcgcctt gcgtcatgcg ctctcccctc ttgtagattg gcaacaggat
    18421 agcaaaaccc ccgtccggcc tcgaccttct gcactctcat cgcgcacgga tgcatgtttt
    18481 atgtgcaact gttttgaccg tttagtccac tttttaccag atattttaga taacttagca
    18541 ttaacacatt aataacattc actttttaaa aactggcatc cactttgcaa acaagccatt
    18601 acgcggctga aaaggatatg caggacgcag tgccccgtct gactttcact ttacgagacg
    18661 aagagaggtt gatgatgaaa attggcgtat tcgtacctat tggcaacaac ggctggctca
    18721 tttcgaccca cgcgccgcag tacatgccga cctttgaact gaataaagcc attgtgcaaa
    18781 aagcggagca ctaccatttc gatttcgccc tgtcgatgat caaactgcgt ggctttggcg
    18841 gcaaaactga gttctgggat cacaaccttg agtcgttcac cttgatggcg gggctggcag
    18901 ccgtcacctc acgcattcag atttacgcga cagctgccac cttaacgtta cctccggcaa
    18961 tcgtcgcccg tatggccgca accattgact ccatctctgg cgggcgtttt ggcgtcaacc
    19021 tcgtgactgg ctggcaaaag cccgagtatg agcagatggg tatctggcct ggcgatgact
    19081 atttctcccg tcgttacgac tatctcaccg aatatgttca ggcgctgcgc gacctgtggg
    19141 gctcggggaa aagcgatttt aaaggcgatt ttttcaccat gaatgattgt cgcgtcagtc
    19201 cgcaaccgag tgtacccatg aaagtgatct gcgccgggca aagcgacgct ggcatggcgt
    19261 tctccgccca gtatgccgat ttcaacttct gtttcggcaa aggcgtaaat acacccacgg
    19321 ctttcgcccc gaccgctgcg cggatgaaac aggccgcaga gcaaaccggg cgcgacgttg
    19381 gctcttatgt attgtttatg gtgattgccg atgaaaccga cgatgccgct cgcgccaaat
    19441 gggaacacta caaagcgggc gcggatgaag aggcgttaag ctggctaacc gaacaaagtc
    19501 agaaagatac ccgctccggt actgacacca acgtccgtca gatggccgat cccacttcgg
    19561 cggtaaacat caatatgggg acgttagtcg gttcttacgc cagtgtcgcg cgcatgttag
    19621 atgaagtcgc aagcgtgcct ggtgccgaag gcgtgctgtt gaccttcgat gattttctgt
    19681 cgggaatcga aaccttcggc gagcgcattc aaccactgat gcagtgccgc gcccatctcc
    19741 ctgcgctgac tcaggaggtg gcatgatgac gaccttaacc gctcgaccgg aagccattac
    19801 cttcgatccg cagcaaagtg cgctgatcgt ggtggatatg caaaacgctt atgccacgcc
    19861 aggaggctac ttagatctcg ccgggtttga tgtctcaacc actcgcccgg tcattgccaa
    19921 cattcaaacc gccgtgaccg cagcgcgagc ggcagggatg ctgatcatct ggtttcaaaa
    19981 tggctgggat gaacagtatg tcgaagcagg cggacccggc tcaccgaatt ttcataaatc
    20041 gaacgccctg aaaaccatgc gtaagcagcc gcagctgcag gggaaattgc tggcgaaagg
    20101 ctcctgggat tatcaactgg tggatgaact ggtgccgcag cctggcgata ttgtgctgcc
    20161 gaagccgcgc tacagcggtt tcttcaatac gccgctggac agcattttgc gcagccgtgg
    20221 aatacgccat ctggttttca ccggtatcgc taccaacgtc tgcgtcgaat cgacgctacg
    20281 cgatggcttt tttctggagt atttcggcgt ggtgctggaa gacgcaacac accaggcggg
    20341 gccggaattt gtacagaaag ccgcgttgtt caatatcgaa accttttttg gctgggtcag
    20401 cgacgtcgaa acgttctgcg acgcgctttc tcccacgtcc tttgctcgta tcgcttaagg
    20461 agtttaacga tgccaaaatc cgtaattatt cccgctggca gcagcgcacc gctggccccc
    20521 ttcgttcccg gcacgctggc tgatggcgtg gtgtatgtct ccggtacgct ggcttttgat
    20581 caacataata acgtgctgtt tgccgatgac ccaaaggcgc aaacccgcca cgttctggaa
    20641 actatccgca cggtgatcga gacggcgggt ggcacgatgg cggatgtgac cttcaacagc
    20701 atctttatta ccgactggaa aaattacgcc gcgattaacg aaatctacgc cgagtttttt
    20761 ccgggtgata aaccggcgcg attctgcatt cagtgcggac tggtaaaacc tgacgcgctg
    20821 gtggaaatcg ccacaattgc gcatatcgcc aagtgaggcc gcgatgaaac tatcactctc
    20881 acctccccct tatgctgatg cacccgtagt ggtgttgatt tcgggtcttg gcggtagcgg
    20941 cagttactgg ttaccgcaac tggcggtgct ggagcaggag tatcaggtag tctgttacga
    21001 ccagcgcggc accggcaata atcccgacac gctggcagaa gattacagta tcgcccagat
    21061 ggcagcggaa ctgcatcagg cgctggtagc cgcagggatt gagcattacg cagtggtcgg
    21121 ccatgcgctc ggtgcgctgg tggggatgca gctggcgctg gattatcccg cgtcggtaac
    21181 tgtgctggtc tgcgttaacg gctggttacg aataaacgcc catacgcgcc gctgttttca
    21241 ggttcgcgaa cgattactgt atagcggcgg cgcgcaggca tgggtggaag cgcagccgtt
    21301 gttcctctat cccgccgact ggatggcggc ccgcgcacct cgccttgagg cagaagacgc
    21361 gctggcactg gcgcattttc agggcaaaaa taatttactg cgtcgactta acgccctcaa
    21421 acgcgctgac tttagtcatc atgcggttcg catccgctgc ccggtgcaaa tcatctgcgc
    21481 cagtgatgat ctgctggtgc catcagcatg ttccagtgaa cttcatgccg ccctgcccga
    21541 tagccagaaa atggtgatgc gctatggcgg acacgcctgc aacgtgaccg atcccgaaac
    21601 gtttaatgct ctgttactca acgggcttgc cagcctgtta catcaccgtg aagccgccct
    21661 gtaaggaatt gctatgaacg aagccgttag cccaggtgcg cttagcaccc tgttcaccga
    21721 tgcccgcact cacaatggct ggcgggagac acccgtcagc gatgagacgt tacgggagct
    21781 ttatgccctg atgaaatggg ggccgacatc agctaattgt tctccggcac gtatcgtgtt
    21841 tatccgcact gcagagggaa aagaacgtct gcgcccggcg ctttccagcg gcaatctgca
    21901 aaaaaccctg accgcgcccg tcaccgctat cgtcgcctgg gacagtgaat tttatgaacg
    21961 gttaccacta ctgtttcccc acggtgatgc ccgcagttgg tttacctcca gcccacaact
    22021 tgccgaagaa acagcgtttc gcaacagttc catgcaggcg gcctatctga tcgtcgcttg
    22081 ccgtgctctg ggactggata ccggcccgat gtcgggtttt gaccgtcaat acgttgacga
    22141 cgcttttttt gcaggcagca cgctgaagag caatctgctg attaacatcg gctatggcga
    22201 taacagcaaa ctttatgcgc gcctgccacg tctgtccttt gaagaagcct gcgggctgtt
    22261 gtaaggagca aactatgaac atagttgatc aacaaacctt tcgcgatgcg atgtcctgta
    22321 tgggcgcggc ggtcaatatc atcaccacgg acggtccagc cgggcgcgcc gggttcaccg
    22381 ccagcgccgt ctgcagcgtg accgacacgc cgcccacatt actggtgtgc ctgaatcgtg
    22441 gggcgtccgt ctggccggta ttcaatgaaa atcgaacgct gtgtgtaaat acacttagcg
    22501 ccgggcagga gccgctttcc aacctttttg gcggcaaaac gcccatggaa caccgctttg
    22561 ccgccgcccg ctggcagacg ggtgtgaccg gatgcccgca actggaagag gctctggttt
    22621 cgtttgactg ccgtatcagc caggtggtga gcgtcggcac ccacgacatt ctgttttgcg
    22681 ccatcgaagc gattcatcgt cacgccacac cctacgggct ggtgtggttc gatcgcagtt
    22741 atcacgcgct gatgcgccct gcttgttaac ctccttaagg agacagctga tggcaatgtt
    22801 cggttttcct cactggcagt taaaatcgac ctctacagag agcggcgtgg tcgcgccgga
    22861 tgaacgattg ccgtttgctc agacggcaat aatgggcgtt caacacgcgg tggcgatgtt
    22921 tggcgcgacg gtgttaatgc cgattcttat ggggctggat cctaaccttt ccattttaat
    22981 gtcgggggtc ggcacgttac tgtttttctt tattaccggc gggcgcgtcc ccagttatct
    23041 cggttccagc gcggcttttg tcggcgtggt gattgccgcg acgggcttta acggtcaggg
    23101 cattaacccg aatatcagca ttgcgctggg cgggattatc gcctgtgggc tggtttatac
    23161 cgtcattggc ctggtggtga tgaaaatcgg tacacgctgg attgaacgac taatgccgcc
    23221 ggtggtgacg ggtgcggtgg tgatggcgat tggcctgaac ttagcgccta tcgcagtgaa
    23281 aagtgtatcg gcttcggcct ttgatagctg gatggcggta atgacggtgc tgtgtattgg
    23341 gctggtggcg gtgtttaccc gtggcatgat ccagcggtta ttgattctcg taggtttaat
    23401 tgtcgcctgt ctgctgtatg gcgtgatgac caacctttta gggctgggaa aagccgtcga
    23461 ttttactctc gtcagccatg ctgcctggtt cggtttgcca catttttcca ctcctgcttt
    23521 taatagtcag gcaatgatgc tgattgcgcc cgtggcggtg attctggtgg cggaaaactt
    23581 aggtcatctc aaggcggtcg ccgggatgac cggacgcaat atggacccgt atatggggcg
    23641 ggcattcgtc ggtgatgggc tggcgacgat gctttctggt tctgtcggtg gcagcggcgt
    23701 aaccacctac gccgagaata tcggtgtaat ggccgtgaca aaagtctact cgacgctggt
    23761 gtttgtcgct gctgccgtca tcgccatgct gttgggcttt tcaccgaaat ttggcgcgct
    23821 gattcatacc attccggccg cggtgattgg tggtgcatca attgttgtgt tcgggctgat
    23881 tgccgtcgca ggcgcaagga tatgggtaca aaaccgtgtc gatttaagcc agaacggtaa
    23941 tttgattatg gtcgcagtaa ccttagtgct gggcgcgggt gattttgctc tcacgctggg
    24001 cggttttacg ttgggaggga ttggtacagc aacctttggc gcgattttac tcaatgcgtt
    24061 gttaagcaga aagttggttg acgttccacc gccagaagtt gttcatcagg agccataaac
    24121 agaaaaccgc cgggattacc ggcggtgatg actttttgac gagcctaaga aaaaaatgca
    24181 ttcgtcattg attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt
    24241 ttacctgctt cagatgcgcg ctgcgggtca ttcttgaaat tacccccgct gtgctgtcca
    24301 ccttttttac ctgcttctga tgctctttcg cggtcttctg caaaattgcc ggaaccgcct
    24361 cgatggtttg ccattactga cctccgtctg ggtatagaca tcatattgca tagaattaac
    24421 gcggcgtatg gcggctgcca tattttcgct acgtgggact aagcttagtg aactgacgcg
    24481 ggcagacccg cgtttggtaa tatcctgcaa cagcttctta gcgagaatag atatcatctc
    24541 aaataattat ccataagccg ctcttatgat taaaaaaacg cgatgagata aaaatgtgtc
    24601 attttgcgac aaaattacgt gcttgtgaaa gttgttataa atcaaataag tggttgtgaa
    24661 atttgcactc tgaaaaggac gtcttatctt taaataagtg gtagcgaatc gctacggaat
    24721 agagataaca cgaggagtgg ttagaaatgg ctaaagttct ggtgctttat tattccatgt
    24781 acggacatat tgaaacgatg gcacgcgcag tcgctgaggg tgcaagcaaa gtcgatggcg
    24841 cagaagttgt cgttaagcgt gtaccggaaa ccatgccgcc gcaattattt gaaaaagcag
    24901 gcggtaaaac gcaaactgca ccggttgcaa ccccgcaaga actggccgat tacgacgcca
    24961 ttatttttgg tacacctacc cgctttggca acatgtccgg tcaaatgcgt accttcctcg
    25021 accagacggg cggcctgtgg gcttccggcg cactatacgg aaaactggcg agcgtcttta
    25081 gttccaccgg tactggcggc ggtcaggaac aaactattac ttcaacctgg acgacccttg
    25141 cgcatcacgg catggtaatt gtccccattg gctacgcagc gcaggaatta tttgacgttt
    25201 cacaggttcg cggcggtacg ccgtacggcg caaccaccat cgcaggcggt gacggctcac
    25261 gccagccaag ccaggaagaa ctgtctattg ctcgttatca aggggaatat gtcgcaggtc
    25321 tggcagttaa acttaacggc taatcttcaa caggaggata cgcatgccaa ctcaagaagc
    25381 gaaagctcat cacgtcggtg aatgggcatc tctgcgcaat acgtcgccgg aaatagccga
    25441 ggccattttc gaagtcgccg ggtacgacga aaaaatggcg gaaaagattt gggaagaagg
    25501 tagcgatgaa gtgttagtca aagcctttgc taagaccgat aaagattcgc ttttttgggg
    25561 cgaacagacc atcgaacgta aaaacgttta accagcgact cccccacttc tcgcggggga
    25621 gttttctgtt atttcaccgc ctcattcaac acgctatcaa acttatccat tgggcagaaa
    25681 ccgttggcat ctatcgggca accgcttaat tccagcgtca cacgctgagc aggtgcctgc
    25741 agggttaacg catcggcatt acgtaactgt tccgcactct gatacacata ttcaattttc
    25801 atcaaatcgc gattggcttt gctgtcatgc caacgctgga aaacgatttt gccgccaatc
    25861 ggcgtgcgtt cgttctggtc atgcaactga tacggtttga aatccagcgc cgttaacaga
    25921 gaggcaatgt tggagtcgtg cccaaccaac actgtaattt tcggtgcgct ggtgcgatcg
    25981 gtgaccagag ctttgtcgat ataactgacc agcggtttcg caacattgcg cgccacttcc
    26041 ggtgaggtaa acaggctgtc ctggtagccg tttttaagct tcgacaacac cttccactgc
    26101 tggtcagatt tgatttctcc ccaggccacc tgatccatcg gaaaaccttc gtaatattgc
    26161 aaagtaaacg catctaccag cgagttgccg actttcagcg gcccggaaac acctggttct
    26221 tgttgatact tggcgctaaa ggtatttttg ccatccacca gcgaacactg ttgtttctct
    26281 ttacaggcag gggaatcttt atagttaacg attttttcca gtagttggta gctgtcggta
    26341 agctggagtt tgctgagctc tttctccatt gccgccaccg cctgttcact gaatgcggcg
    26401 gaatcatcgg tgatcaccgg gttaaaggtt gggtccatag tgcccatttt ttcctggtga
    26461 tgcacaggaa tatcacaccc cgggaatgcg ccggtaataa agaactgtgc ggtcgcaacg
    26521 gtacgttgca gactattggc ataggcataa acggtgtccg gcggcgggca ttcccccgat
    26581 ttcaccatcc cctgctgtgc cagccattca cgcatgtaat ggcccatata cacttcgagc
    26641 acgccacctt tggtggtgag ttgcccaccg gggacgtccc attcgggcca tttattcggc
    26701 gtagactgct ccagcacact gccattgttc gccagcggcg cacgtaagtt atggcggctc
    26761 atcatgagca cttgctgtag ctgatagcct tccggtacgg tttgtgcctg agcatttgaa
    26821 gcgagtaaaa ctatccctgc cacagttgcg gcgattagcg ttttgttcat tcctggcacc
    26881 tcttttgtta tcaatcacta aagagtgtga cagaaatatg acatttttcc gggaacaggt
    26941 tcgcaaaatg gcttgttgaa tgcctgtttt tctatataca tctttaaaaa caggcaaggt
    27001 ttgacgcaaa ctatgcctga ttagctccct gcctcgcgta cgccgctgat gattacacct
    27061 gtggctaaac agaatatcag cggcgttact aatacagagg caatagtacc aataataaga
    27121 gaagtgaggc taataacaat ggtttttagc acatgccttt gtcagtaaat tgcataacga
    27181 gttaaaaaat caatttgcgc attcctttcc ttattgttta actcgtaagc ctgagcaaaa
    27241 agttcaggag ggaggtaaac gtcataatga ttgccagtgt ttattaaata cccatactca
    27301 aggtcgtcat ctgttgaaaa ggattcattc cctaaggaga ttgatgccac atta
//
LOCUS       DAFNBJ010000057        26762 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000057 DAFNBJ010000000
VERSION     DAFNBJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.057"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(280..2853)
                     /gene="clpB"
                     /locus_tag="L2X49_002236"
     CDS             complement(280..2853)
                     /gene="clpB"
                     /locus_tag="L2X49_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBN4797318.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2983..3714)
                     /gene="pgeF"
                     /locus_tag="L2X49_002237"
     CDS             complement(2983..3714)
                     /gene="pgeF"
                     /locus_tag="L2X49_002237"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBN4797319.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3711..4691)
                     /gene="rluD"
                     /locus_tag="L2X49_002238"
     CDS             complement(3711..4691)
                     /gene="rluD"
                     /locus_tag="L2X49_002238"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBN4797320.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIIINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4826..5563
                     /gene="bamD"
                     /locus_tag="L2X49_002239"
     CDS             4826..5563
                     /gene="bamD"
                     /locus_tag="L2X49_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBN4797321.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5834..6175
                     /gene="raiA"
                     /locus_tag="L2X49_002240"
     CDS             5834..6175
                     /gene="raiA"
                     /locus_tag="L2X49_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBN4797322.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6278..6403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6279..6326
                     /gene="pheL"
                     /locus_tag="L2X49_002241"
     CDS             6279..6326
                     /gene="pheL"
                     /locus_tag="L2X49_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HBN4797323.1"
                     /translation="MKHTPFFFAFFFIFP"
     gene            6425..7585
                     /gene="pheA"
                     /locus_tag="L2X49_002242"
     CDS             6425..7585
                     /gene="pheA"
                     /locus_tag="L2X49_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBN4797324.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTCLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7628..8749)
                     /gene="tyrA"
                     /locus_tag="L2X49_002243"
     CDS             complement(7628..8749)
                     /gene="tyrA"
                     /locus_tag="L2X49_002243"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBN4797325.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8760..9830)
                     /gene="aroF"
                     /locus_tag="L2X49_002244"
     CDS             complement(8760..9830)
                     /gene="aroF"
                     /locus_tag="L2X49_002244"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBN4797326.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10040..10405
                     /locus_tag="L2X49_002245"
     CDS             10040..10405
                     /locus_tag="L2X49_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBN4797327.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10555..11073
                     /locus_tag="L2X49_002246"
     CDS             10555..11073
                     /locus_tag="L2X49_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBN4797328.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11063..12289
                     /gene="dgcN"
                     /locus_tag="L2X49_002247"
     CDS             11063..12289
                     /gene="dgcN"
                     /locus_tag="L2X49_002247"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HBN4797329.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12305..12787
                     /locus_tag="L2X49_002248"
     CDS             12305..12787
                     /locus_tag="L2X49_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBN4797330.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12863..13210)
                     /gene="rplS"
                     /locus_tag="L2X49_002249"
     CDS             complement(12863..13210)
                     /gene="rplS"
                     /locus_tag="L2X49_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBN4797331.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13252..14019)
                     /gene="trmD"
                     /locus_tag="L2X49_002250"
     CDS             complement(13252..14019)
                     /gene="trmD"
                     /locus_tag="L2X49_002250"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBN4797332.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14050..14598)
                     /gene="rimM"
                     /locus_tag="L2X49_002251"
     CDS             complement(14050..14598)
                     /gene="rimM"
                     /locus_tag="L2X49_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBN4797333.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14617..14865)
                     /gene="rpsP"
                     /locus_tag="L2X49_002252"
     CDS             complement(14617..14865)
                     /gene="rpsP"
                     /locus_tag="L2X49_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBN4797334.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15002..16363)
                     /gene="ffh"
                     /locus_tag="L2X49_002253"
     CDS             complement(15002..16363)
                     /gene="ffh"
                     /locus_tag="L2X49_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBN4797335.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16530..17321
                     /locus_tag="L2X49_002254"
     CDS             16530..17321
                     /locus_tag="L2X49_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBN4797336.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17342..18628
                     /locus_tag="L2X49_002255"
     CDS             17342..18628
                     /locus_tag="L2X49_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBN4797337.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18683..19276)
                     /gene="grpE"
                     /locus_tag="L2X49_002256"
     CDS             complement(18683..19276)
                     /gene="grpE"
                     /locus_tag="L2X49_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBN4797338.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRIKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19399..20277
                     /gene="nadK"
                     /locus_tag="L2X49_002257"
     CDS             19399..20277
                     /gene="nadK"
                     /locus_tag="L2X49_002257"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBN4797339.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20363..22024
                     /gene="recN"
                     /locus_tag="L2X49_002258"
     CDS             20363..22024
                     /gene="recN"
                     /locus_tag="L2X49_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBN4797340.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22173..22514
                     /gene="bamE"
                     /locus_tag="L2X49_002259"
     CDS             22173..22514
                     /gene="bamE"
                     /locus_tag="L2X49_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBN4797341.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22576..22866)
                     /locus_tag="L2X49_002260"
     CDS             complement(22576..22866)
                     /locus_tag="L2X49_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBN4797342.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22856..23293)
                     /gene="ratA"
                     /locus_tag="L2X49_002261"
     CDS             complement(22856..23293)
                     /gene="ratA"
                     /locus_tag="L2X49_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBN4797343.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            23464..23946
                     /gene="smpB"
                     /locus_tag="L2X49_002262"
     CDS             23464..23946
                     /gene="smpB"
                     /locus_tag="L2X49_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBN4797344.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24161..24523
                     /gene="ssrA"
                     /locus_tag="L2X49_002263"
     tmRNA           24161..24523
                     /gene="ssrA"
                     /locus_tag="L2X49_002263"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24690..25919
                     /locus_tag="L2X49_002264"
     CDS             24690..25919
                     /locus_tag="L2X49_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014641070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN4797345.1"
                     /translation="MAKIAKKLTDTEIKSTKPAEKEVNLFDGDGLLLRIAPLAKGGKK
                     NWYFRYAVPVTKKRTKVSLGTYPHLTLAKARALRDEYLSLLTNGIDPQVHNNQKANAL
                     KDATEHTFQAVAKKWLDEKVKTSGISQDHANDIWRSLERNIFSTLGDTPIKEIRPKML
                     KQHLEPIEKRGVLETLRRIISRLNEIFRYAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFLVALNNASIRLETRLLIEWQLLTWVRPGEAVRTRWSDIDIETGMWNIPA
                     EFMKMKKPHKVPLSKEALRVLDLMKVISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRTDVLEAALAHSKKDEIIAAYNRAEYLTERVVLM
                     QWWSDYVSSQKCKVIAA"
     gene            26202..>26762
                     /locus_tag="L2X49_002265"
     CDS             26202..>26762
                     /locus_tag="L2X49_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBN4797346.1"
                     /translation="MASENDKNHRVRVAQYLRMSTDHQQYSLHNQSEYIKDYAEKNNM
                     EIAYTYDDAGKSGVSIVGRHSLQQLLSDVEQKKIDIQAVLFYDVSRFGRFQNSDEAAY
                     YSFLFERNGVDLIYCSEPIPTKDFPLESSVILNIKRSSAAYHSRNLSEKVFIGQVNLI
                     KLGYHQGGMAGYGLRRLLVDENGIAKE"
BASE COUNT         6906 a         6976 c         6256 g         6624 t
ORIGIN      
        1 cagcggcgcg gggtgtgcat aatacgcttt cccgctacag agtcaagcat ttttttgctt
       61 ttctctgttg agattctcag gagaaccccg ctgccccggc ggcgtgtttg ccgttgttcc
      121 gtgtcagtgg tggcgcatta tagggagtta ttccggcctg acaagcgaaa aagataaaaa
      181 ctttatcgtt cgctcatttt tcaggcaaaa catcttaaat atagtctttt ccgtctaact
      241 tatagacaaa aacgagcccc gaagggctcg ttttatcatt tactggacgg cgacaatccg
      301 gtcttcatta acttccaggc gaatcacttt acccggaacc aattcaccag acagtatttg
      361 ctgtgccagc gggttttcga tctgctgctg aattgcacgt ttcagaggac gtgcaccata
      421 gaccggatcg taaccgttct cgctcagcag tttcagcgcc tcgtcagaaa tgtggatttc
      481 ataaccacgt tcttccagac gtttatacag acgtttcaac tgaatctgcg caatcgacgc
      541 aatgtgctgt tcacccagcg gatggaagac caccacttca tcgatacggt taatgaattc
      601 cggacggaag ttatggctaa ccacaccgag caccagctct ttcatgtgcg cataatccag
      661 ttcaccgaag cgttcctgaa tcagatcgga accgaggtta gaggtcataa tgacgaccgt
      721 attacggaag tcgaccgttc tcccctgccc gtcagtcaga cgcccatcat ccagtacctg
      781 caacagaatg ttgaagacat ccggatgcgc tttttccact tcatccagca ggatgacgga
      841 atacggacga cgacgcaccg cttcggtcag gtagccacct tcttcataac cgacatatcc
      901 cggaggcgca ccaaccaaac gagacaccga gtgtttctcc ataaactcgg acatatctat
      961 acggaccatc gcctcgtcgc tatcaaacat aaagttcgcc agcgccttac aaagctctgt
     1021 tttccccaca cccgttgggc cgaggaacag gaatgaacca atcgggcgat ttggatccgc
     1081 caaccccgca cggctacggc gaatagcgtt agataccgca tcaaccgctt cgttctgacc
     1141 aatgacgcga tggtgcagtt cttgctccat acgcagcagt ttttcgcgct cgctttccat
     1201 catgcgagaa accggaatcc ccgtccaacg cgccagcact tcagcaattt cggcgtcggt
     1261 cactttatta cgcaacagac gcatagtttt gccttcgagc tgcgttgcgg cttccagttg
     1321 cttttccagt tccgggattt tgccgtattg cagttcagac atccgcgcca ggtcccccac
     1381 acggcgagcc tgttcaatag cgattttcgc ctgttccagt tccgctttaa tggtctgcgt
     1441 accagaaagc gatgccttct ctgctttcca ctcttcttct aactcggagt actgacgttc
     1501 tttgtcgctc agttcttcgt tgagcatatc caggcgtttt ttactggctt catcagactc
     1561 tttcattaac gcctgttgtt ccagtttgag ctggatgata cgacgatcga gtcggtcgag
     1621 ttcttctggt tttgagtcaa tctgcatacg aatgctggat gctgcttcat cgatcaggtc
     1681 gatggcttta tccggcagct gacggtcagc aatgtagcga tgagacaacg tcgccgctgc
     1741 aacaattgcc gggtcagtaa tttgcacatg gtggtgcaat tcgtaacgtt ctttcaggcc
     1801 acgcagaatc gcaatggtat cttcaacaga aggctcggca acaaacactt tctggaaacg
     1861 acgttccagc gcagcatctt tttcgatgta ctggcgatat tcgtcaagcg tcgtggcacc
     1921 tacgcagtgc aattcaccac gcgccagcgc cggtttcagc atgtttccgg cgtccattgc
     1981 gccatcggct ttacccgcgc cgaccatggt atgtaattcg tcgataaaca ggatgacgtt
     2041 gccttcctgt ttggcaagat cgttaagcac gccttttaaa cgttcttcaa actcaccgcg
     2101 atatttcgcc ccagccacca gcgcgcccat atccagcgcc agtacgcggc ggcctttcaa
     2161 tccttccggc acttcgccgt tgataatacg ctgcgccaga ccttcaacga tggcagtttt
     2221 accgacgccg ggttcaccaa tcagtaccgg gttattttta gtacgacgtt gcagcacctg
     2281 aatggtacgg cgaatttctt catcacgacc aatcaccgga tcgagtttgc cctgttcggc
     2341 tcgttcggta aggtcgatgg tatatttttt caaagcctga cgttggtctt cagcaccttg
     2401 atcgttcacg ctttcacctc cacgcatttg ttcaatcgct tgagtaatgt tggcggtggt
     2461 cgcccctgct gctttcagga tgtcggccag cgtgccgcga gactcaagtg ccgccagaac
     2521 gaacagttct gacgagataa agttatcacc acgtttttgc gccagcttgt cgcaaagatt
     2581 aagaacgcgc accagatcct gtgatggctg gacatcacca ccagtacctt caacctgcgg
     2641 taaacgattt aatgcctgat tgatatctgt gcgcaactgg ccagcattta tgccagctga
     2701 tgttaacaaa ggactaaccg aacccccttc ctgattcagc agggcgctca ttaaatgaag
     2761 tggttcgatg aattggttgt cgtgcccgag tgcaagtgat tgggcatcgg caagagcaag
     2821 ctggaattta ttagtaagac gatccagacg cataactcct cccataacgg atcaaagttg
     2881 ctactggaga ttaaatgagg tcatccctca attattcaag gttattgacc agattaatgt
     2941 gaaaagaaaa tcacgcgtac cggatcgtct tgattcttta ggttatatca gccaaatgaa
     3001 acttgccata cgcccggtgg tcttgtcgcg acgataagag aagaaagtct cattttccgt
     3061 atatgtacaa cggtcgccgc cgaaaatttg ttcaacacca acgctcgcca gacgctgccg
     3121 ggcaagcaga taaatatccg ccagatactt atcaccatgc tgaatgaaag ctgtactcgc
     3181 tttggcgtct gctgccataa acgcctcgcg aacctccgcc cccacttcga acgcgcgtgg
     3241 accaattgcc ggccctaacc aggcgagaat attttccgga ttatcagcaa aacaggaaac
     3301 tgtctcttcc agcacgcctg cgcacagtcc acgccagcca gcatgagcgg cggcgacttc
     3361 cgttcccgct cgattgcaaa acagcacagg gaggcagtcg gcagtcatca ctgcgcaaac
     3421 cgtgccaggc gtattgctat aagaggcatc tgctcgtttt gaggcataag gttcgccagt
     3481 aagcttaagc acatctttgc cgtgtacctg ctcaagccag accggtttag aaggcaaatt
     3541 gcccgcagca aaaagtcgct tgcgattctc ctcaacgtga tccgggttat cgccacaatg
     3601 ggcaccgagg ttgagcgagt catacggggg caagctcacg ccgccgatac gagtagagct
     3661 acaagccgca acaccctttg gcagcggcca ctgcgggaca atcagcttac tcataaccag
     3721 tccacttcat ccttatgttc ttcgaaatcg gcgcgcatca cctcaatcag ctccaccata
     3781 tcttgtggaa taggcgcatg ccattccatt tcgatgccgg agatcgggtg ataaagacgc
     3841 agcatggttg catgtagcgc ctggcggtca aacttacgca gcgtggagat aaatgcttcc
     3901 gaagcacctt ttggcggacg cggacggcca ccataaaccg gatcgcccac cagcggatga
     3961 gtgatatggg ccatatgcac gcggatctgg tgcgtacgtc cagtttccag acgcaaccgc
     4021 agacgcgtgt gcacacggaa gtgttccatg atgcgatagt gagtcaccgc tggtttgccc
     4081 atcggatgca ccgccatatg ggtacgtttg gtcgggtggc gactgattgg ctcgtccacc
     4141 gtgccacctg cggtcatatg accaatcgcc accgcttcat actcacgagt aatttcacgc
     4201 cgttgcaaag attcgactaa acgcgtctga gccggaacgg tttttgccac aaccatcagg
     4261 ccagtggtgt ctttatccag acgatggacg atgcccgcac gcggtacatc ggcaatgggt
     4321 ggatagtaat gaagcaacgc attcagtacc gtgccatccg ggttacccgc gccaggatgt
     4381 accaccaggt cgcgcggttt attaatgata ataatgtctt catcttcata gacgatatcc
     4441 agcgggatat cctgcggttc aaaacgcgct tcttcttcaa tctcagcgtt gatggcaacc
     4501 tgctcgccac ccaatacttt ttctttcggc ttatcacaaa ctttgccgtt aaccagcact
     4561 cgctggtcga ggatccattc ttttattcgc gaacgtgaat aatccgggaa catttcggcc
     4621 aaagcctgat ctaagcgttg accgagttgg ttttcggaca ccgttgcagt gagctgtact
     4681 cgttgtgcca tatatactgc ttcttcgttt atcgttgggg ttttacggct ttgccgttta
     4741 atatagtgtg ctattgtagc tggtcttaac cgggagcagg aacagagaat ctcccgtatt
     4801 acattttgag gaaagtcaaa acgtcatgac gcgcatgaaa tatctggtgg cagccgccac
     4861 actaagcctg tttttggcgg gttgctcggg gtcaaaggaa gaagtacctg ataatccgcc
     4921 aaatgaaatt tacgcgactg cacaacaaaa gctgcaggac ggtaactgga gacaggcaat
     4981 aacgcaactg gaagcgttag ataatcgcta tccgtttggt ccgtattcgc agcaggtgca
     5041 gctggatctc atctacgcct actataaaaa cgccgatttg ccgttagcac aggctgccat
     5101 cgatcgtttt attcgcctta acccgaccca tccgaatatc gattatgtca tgtacatgcg
     5161 tggcctgacc aatatggcgc tggatgacag tgcgctgcaa gggttctttg gcgtcgatcg
     5221 tagcgatcgc gatcctcaac atgcacgagc tgcgtttagt gacttttcca aactggtgcg
     5281 cggctatccg aacagtcagt acaccaccga tgccaccaaa cgtctggtat tcctgaaaga
     5341 tcgtctggcg aaatatgaat actccgtggc cgagtactat acagaacgtg gcgcatgggt
     5401 tgccgtcgtt aaccgcgtag aaggcatgtt gcgcgactac ccggataccc aggctacgcg
     5461 tgatgcgctg ccgctgatgg aaaatgcata ccgtcagatg cagatgaatg cgcaagctga
     5521 aaaagtagcg aaaatcatcg ccgcaaacag cagcaataca taacagaaac ctaaaacaca
     5581 aaacggcagc ccttgagctg ccgttttttt attctgtcag ttgtgaaact gaaccgattt
     5641 agtcgctatc gatctcatca aatatggctc gctttgagat attcctcaag taaaaaaaca
     5701 cctcttcctg cgatttctca caaaaaagat tcgttgacaa aaagtgacaa aattatgaga
     5761 ttttcatcac acattttgac atcaggaacg gtatgctgaa ttcaccaaga cgggaagaca
     5821 agaggtaaaa tttatgacaa tgaacattac cagcaaacaa atggaaatta ctccggccat
     5881 ccgccaacat gtcgcagacc gtctcgccaa actggaaaaa tggcaaacac atctgattaa
     5941 tccacatatc attctgtcca aagagccaca agggtttgtt gctgacgcca caatcaatac
     6001 acctaacggc gttctggttg ccagtggtaa acatgaagat atgtacaccg caattaacga
     6061 attgatcaac aagctggaac ggcagctcaa taaactgcag cacaaaggcg aagcacgtcg
     6121 tgccgcaaca tcggtgaaag acgccaactt cgtcgaagaa gttgaagaag agtagtcctt
     6181 tatattgagt gtatcgccaa cgcgccttcg ggcgcgtttt ttgttgacag cgtgaaaaca
     6241 gtacgggtac tgtactaaag tcacttaagg aaacaaacat gaaacacaca ccgtttttct
     6301 tcgcattctt ttttatcttc ccctgaaagg gaggcgtttc gtcgtgtgaa acagaatgcg
     6361 aagacgaaca ataaggcctc ccaaatcggg gggccttttt tattgataac aaaaaggcaa
     6421 cactatgaca tcggaaaacc cgttactggc gctgcgagag aaaatcagcg cgctggatga
     6481 aaaattatta gcattactcg cagagcggcg cgaactggcc gtcgaggtgg gaaaagccaa
     6541 actgctctcg catcgcccgg tacgtgatat tgatcgtgaa cgcgatttac tggaaagatt
     6601 aattacgctc ggtaaagcgc accatctgga cgcccattac attactcgcc tgttccagct
     6661 catcattgaa gattccgtat taactcagca ggctttgctc caacaacatc tcaataaaat
     6721 taatccgcac tcagcacgca tcgcttttct cggccccaaa ggctcctatt cacatcttgc
     6781 cgctcgtcag tacgctgccc gtcactttga gcaattcatt gaaagtggct gcgccaaatt
     6841 tgccgatatt tttaatcagg tggaaaccgg ccaggccgac tatgccgtcg taccgattga
     6901 aaataccagc tccggtgcca taaacgacgt ttacgatctg ctgcaacata cctgcttgtc
     6961 gattgtgggc gaaatgaccc ttaccatcga ccactgcctg ctggtttccg gcaccacaga
     7021 tttatctacc atcaacacgg tctacagtca tccacagccg ttccagcaat gcagcaaatt
     7081 ccttaatcgt tatccgcact ggaagattga atataccgaa agtacgtctg cggcaatgga
     7141 aaaggttgca caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac
     7201 tttgtacggt ttgcaggtac tggagcgtat tgaagcgaat cagcgacaaa acttcacccg
     7261 atttgtggtg ttggcgcgta aagccattaa cgtgtctgat caggttccgg cgaaaacgac
     7321 gttgttaatg gcgaccgggc aacaagccgg tgcgctggtt gaagcgttgc tggtgctgcg
     7381 caaccacaat ctgattatga cccgtctgga atcacgcccg attcacggta atccatggga
     7441 agagatgttt tatctggata ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt
     7501 gaaagagtta ggggaaatta cccgttcaat gaaggtattg ggctgttacc caagtgagaa
     7561 cgtagtgcct gttgatccaa cctgatgaaa aggtgccgga tgatgtgaat catccggcac
     7621 tggattatta ctggcggttg tcattcgcct gacgcaataa cacgcggctt tcactctgaa
     7681 aacgctgtgc gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca ataaacgcct
     7741 gcttatcgcc ctgctccagc aactcaatcg cctcgccgaa acgcttatag taacgtttga
     7801 ttaacgccag attacgctct gacgacataa taatgtcggc ataaagctgc ggatcctgag
     7861 caaacagtcg cccgaccatc gccagctcaa ggcggtaaat cggcgaagag agcgccagaa
     7921 gttgctcaag ctgaacattt tcttctgcca gatgcagccc ataagcaaaa gtagcaaagt
     7981 ggcgcagagc ctgaataaac gccatattct gatcgtgctc gacagcgcta atacgatgca
     8041 gccgagcgcc ccagacctga atttgctcca gaaaccattg gtatgcttcc ggcttacgtc
     8101 catcacacca gaccacaact tgctttgcca ggctaccgct gtccgggccg aacatcgggt
     8161 gtaaccccag taccgggcca tcgtgcgccg ccagcatggc ctgtaatggt ccatttttca
     8221 ctgatgccag atcaaccaga atacaatctt tcggtaaagg cggtaatttg ccgataactt
     8281 gctcagtaac gtggattggc acactaacaa tcaccattcc ggcatcggca acaatatcag
     8341 ccgctcgatc ccagtcatgt tgctccagaa tccgcacctg ataacccgat agtgtcagca
     8401 tcttctcgaa caggcgtccc atctgaccgc caccgccgac gataaccacc ggacgcagtg
     8461 acggacaaag tgttttaaat cctttgtcgt tttcactgga gtaagattca cgcatcaccc
     8521 gacgcaaaac atcctcaatc agatctggcg gtacacccag agcttccgcc tctgcgcgcc
     8581 gcgaggccaa catagatgcc tcgcgctccg gaacataaat aggcagtcca aagcggcttt
     8641 tcacctcgcc cacttcagca accagttcca gacgcttcgc taataaattc agcagcgctt
     8701 tatcgacttc atcaatttga tcgcgtaatg cggtcaattc agcaaccata ataaacctct
     8761 taagccacgc gagccgtcag ctgcccgttc agatcctgat gaatttcacg cagcaaggca
     8821 tcggtcattt cccagctaat gcaggcatcg gttacggata caccgtattt catttcactg
     8881 cgcggttgct cggaagactg attgccctcg tggatattac tttcgatcat cagaccaata
     8941 attgagcgat tgccatcttt gatttgagca accacggatt ctgccaccgc aggctgacgg
     9001 cgataatctt tattggaatt accgtggctg caatctacca tcagagacgg gcgcagtccc
     9061 gcctgttcca tctctttttc acattgcgca acatccgcag ggctatagtt cggcgcttta
     9121 ccaccgcgca ggatcacatg gccgtccgga ttcccctgag tttgtagcaa cgcaacctgc
     9181 cctgcctggt taatgccaac aaaacggtgc ggctgggcgg cggcgcgcat agcgttaatt
     9241 gctgttgcca gactgccgtc ggtgccgttt ttaaaaccaa ccggcatgga aagcccggag
     9301 gccatttcac ggtgagtttg cgattccgtt gtacgagcac caattgctga ccagctaaac
     9361 agatcgccca ggtattgcgg gctattcgga tctaacgctt ccgtcgccag tggcagtccc
     9421 atattcacca gctcaagcag caatttacgc gcgatctgca gcccggcttc tacatcaaaa
     9481 gagccatcca tatggggatc gttaattaac cctttccagc cgacagtggt acggggtttt
     9541 tcaaaataga cgcgcattac cagatagagg ctatcgctga cctctgcggc aagggcttta
     9601 aatcgacgag catattccag agcagtttcc ggatcatgaa tggaacaagg accacatact
     9661 accagcagac gaggatcgcg cccggcgata atatctgaaa tggttttacg cgagtcagca
     9721 atctgggctt cttgttgcag gctcaatgga aaagcggcct tcagttgttc cggagtcatt
     9781 aaaacctgtt cgtcggtaat atgtacgtta ttcagcgcgt ctttttgcat gatggcgatc
     9841 ctgtttatgc tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg ataacacata
     9901 aagtaaagtt ttcaatccat atttcgtaca tttttattta cacaggcaat ttagtcgcga
     9961 tttcaaccct tacttctgta tagataaatt tacactccct ttgaaaacaa tccgctatgc
    10021 tttgaaaaag gagaaagaaa tgatgaaaaa gtttatcgcc cccttgttgg ctttactggt
    10081 tagcggatgt cagattgatc cttatactca cgcgccaacc ttgaccagca ccgactggta
    10141 tgatgttggt atggaagatg cgatatcggg cagcgccata aaagatgacg atgcatttag
    10201 cgattcacag gcggatcgcg gtctatacct taaaggatat gccgaaggac aaaagaaaac
    10261 ttgccagacc gattttactt atgcccgagg actttccggt aaaagctttc ctgccagctg
    10321 caacaatgtt gaaaatgcca gccaactgca tgaagtatgg caaaaagggg ctgatgaaaa
    10381 tgccagcaca atacgattaa attaattaaa accccacaaa taaattaaac ataagatttt
    10441 actcattgtg ttttatttct cacattgatg acgatcgcat gaaaaaatga tattttaatg
    10501 agcattctga aaaaatatcg atgcatttcg agcgaagatg gtggggatcc ctgaatgcgt
    10561 ttttctcacc gactgttctt acttctgatt ctcctgctga cgggagcacc aatactcgct
    10621 caggagcctt ctgacgttgc gaaaaacgtg cgtatgatgg tttccggtat tgtcagttac
    10681 acccgctggc ctgcgctatc agggccacca aagttatgta tcttttcatc ttcacgtttt
    10741 agtaccgccc tacaagagaa tgccgcgaca tctttgcctt atctgcctgt catcattcac
    10801 actcaacaag aggcgatgat ttcaggctgc aatggttttt attttggcaa cgagtcaccg
    10861 acattccaga tggaattaac cgaacaatat ccgtcaaagg cgttattatt aattgccgaa
    10921 caaaataccg agtgcattat tggtagcgcc ttttgtctga tcatccacaa taatgacgtc
    10981 agatttgccg taaacctgga tgccttatcg cgtagcggtg taaaggtcaa cccggatgtc
    11041 ttaatgctcg cacggaagaa aaatgatgga taacgataat tctcttaata agcgccccac
    11101 gtttaaaaga gcattacgca acatcagtat caccagcata tttatcacta tgatgctgat
    11161 ctggttgctg ctttccgtga cctcggtgct gaccctgaaa cagtacgcgc aaaaaaacct
    11221 ggcactgaca gcagcaacaa tgacttacag tctggaagca gctgtcgttt ttgccgatgg
    11281 ccctgcagca actgaaacac tggcagcgct gggccagcaa gggcaatttt caactgcaga
    11341 agtacgtgat aagcagcaaa atattctggc gtcctggcat tacacccgta aggatccagg
    11401 cgatactttc agcaatttca taagccactg gctcttcccc gcccccatca ttcagccgat
    11461 tcgtcacaat ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag
    11521 ccattttatc tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat
    11581 cgcaattacc ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac
    11641 cgatgtcgta catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg
    11701 tatcgctgag tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga
    11761 gtggcagctt cgtttacagg ctaaaaatgc gcagcttcta cgtaccgcgc tacatgaccc
    11821 attaaccggg ctggctaacc gcgcagcgtt tcgtagcggc atcaacacgt tgatgaacaa
    11881 ttccgatgcc cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatacat
    11941 caatgatacc tggggtcatg cgacgggcga tagagtcttg attgaaatcg caaaacggtt
    12001 agctgaagtt ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat
    12061 ggtgctctat gatgtacagt cagaatctga agtgcagcag atatgctcag cactgacaca
    12121 aatctttaat ctcccgtttg atcttcataa tggtcatcag accaccatga cattaagcat
    12181 tggttacgcg atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca
    12241 caatatgtat caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat
    12301 cgatatgata aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg
    12361 ccagtcccct cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt
    12421 tactgaatcc gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa
    12481 tgactacaaa ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc
    12541 ctcgacaggg ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag
    12601 ttacaacgaa ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctatggg
    12661 tggacaaatt ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc
    12721 cagtaacaag accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattactac
    12781 cccttaaaac accagcaaac agataaaaaa gggccagcca attggccagc ccttcttaac
    12841 aggatgtcgc ttaagcgaaa tcttagttaa gacgctcttt gatacgagca gccttaccag
    12901 tacgctcacg caggtagtac agtttagctt tacgaacagc accacgacgt ttgacagaaa
    12961 tgctgtcaac taccggagag tgagtctgga agacacgctc aacgccttcg ccgttggaaa
    13021 ttttacgaac agtgaatgca gagtgcagac cgcggttacg aatagcgata accacgccct
    13081 cgaatgcctg cagacgtttt ttggaacctt caacaaccca tactttcact tccacggtat
    13141 cacccggacg gaaggaaggt acgtcctgct tcatctgctc ttgttcaagt tgcttaataa
    13201 tgttgctcat aatttaatct cttatcctgg gtaaactgat atctcggggg cttacgccat
    13261 cccatcatgt ttatgttgct gttgtgcgtg ttccgttttg aactccgcca gcaaccttgc
    13321 ttgctcttca gtcagagcca ggttttccag aagttcaggt cttctaagcc aggtacggcc
    13381 cagcgactgt ttcaaacgcc agcgacgtat ctcggcatgg ttgcccgaca gtaacactgg
    13441 cggaacttcc atcccttcta acacctcagg ccgcgtatag tgcgggcaat ccagtaatcc
    13501 ttcagcaaag gaatcttccg ttgccgaggc ttcatgtccc agtactcccg gaataaaccg
    13561 ggaaacggag tcaatcagcg tcattgctgg taactcacca ccactgagaa cgtaatcgcc
    13621 gattgaccat tcttcgtcaa tttcggtttg gatcacgcgc tcatctatac cttcgtagcg
    13681 accgcacacc agaatcaatt tttgattcgt tgccagttcg ctgacgcccg cttgatcaag
    13741 cttgcgtccc tgtggtgaca gataaatcac ctttgcgcct tcacccgccg cggcttttgc
    13801 tgcatgaatg gcgtcccgca agggttgcac catcattaac atccccggtc cgccgccgta
    13861 aggacgatcg tccacggtac ggtgccggtc atgcgtgaag tcgcgaggac tccagctctg
    13921 gatgctcagc aggccatttt taactgcccg gccagttacc ccgtaatcgg taattgcgcg
    13981 gaacatttca ggaaacaggc taattatgcc aatccacata gcgccgtctt ttaccgttta
    14041 tccggtggtt taaaaaccag gatcccaatc tacttcgatt gaacgagtag tgagatcgac
    14101 tttcttgata acctgcccat cgaggaacgg tacgagacgt tccttgatac caaacgcatc
    14161 tttcaggttt gccttaatga cgagaacgtc attagatccg gtttccatca tatcgacgac
    14221 tttaccgaga tcgtagcctt cagtggttac tacctggcag cccatcaggt ctttccagta
    14281 gtagtccccc tcttcaagct gaggcagctg cgatgaatcc acgacaattt cacaattcgt
    14341 cagcaggttc gccgcatcac gatcgtcaac gcctttcagc ttgatgatca tgtcctgatt
    14401 gtggtgcttc cagctttcca gctggacttg ctgccactga cccgccttct ggataaacca
    14461 gggctgatag tcaaaaatgc tttcggcgtc ttcggtggaa gaaaacactc tgagccaccc
    14521 acgaataccg taagacgaac ccatttttcc caaaacgatg ggatcaacag gtgcttgcgc
    14581 ggtgagttgt ttgctcatca tgaccaccgt gacagattaa gctgctttgt ttacttcttt
    14641 gatcagcgca gcaacgcgat cagaaatagt tgcgccctgg ccaacccagt gagcgatgcg
    14701 atccagatcc aggcgagtgc cttcttcttt ttcgctagcg attgggttga agaaaccaac
    14761 gcgctcgatg aagcgaccgt tgcgtgcatt acggctgtca gcgacaacaa cctggtagaa
    14821 cggacgcttt ttagcgccgt gacgtgctaa acgaatagtt accataacat cctcttgtgt
    14881 gaataaaaca accggacccc atcgaggaac ggagtccggt gtcatattaa aagcccgaaa
    14941 attttactga tttctggaga aaatgcaatc agcagttaat aactctgtcg cataaggtca
    15001 tttagcgacc aggaaatccc ggcggcatca tccccttcat gcttctcatc atcttcgcca
    15061 ttccgccctt cttcattttc ttcatcatgc gctgcatgtc gtcgaactgc ttcagaaggc
    15121 ggttaacgtc ctgcacctgc ataccgcaac cggcagcgat acgacgctta cgcgaacctt
    15181 tgatgatttc tggcttagcg cgctctttca tcgtcatcga gttgatgatg gcttccatac
    15241 gcaccagcac tttatcgtcc atctgcgact taacattatc cgggatctgc cccatgcccg
    15301 gcagcttgcc catcaggcta gccatgccgc ccatattttt catctggcgc agttgctcaa
    15361 gaaaatcgtt gagatcgaag ccgtcgcctt ttttcagctt gctggctaat ttctctgcct
    15421 gcgcgcggtc aactttgctt tcgatatctt cgatcagcga cagtacgtcg cccatgccga
    15481 gaatacgcga cgcgatgcgg tccggatgga acggctccag cgcctcagtt ttctcgccaa
    15541 caccgagaaa cttgatcggt ttgccagtga tgtgacgaat agagagcgcc gcaccgccgc
    15601 gggcatcgcc gtccactttg gtcaacacta cgccggtaag cggtaacgct tcattgaatg
    15661 cttttgccgt attggccgca tcctgaccgg tcatggcgtc aaccacaaac agggtttcaa
    15721 ccgggttaat cgacgcatgg acttgtttga tctcgtccat catcgcttcg tcaacgtgca
    15781 gacgaccagc ggtatccacc agcagcacgt cgtagaattt cagtttggct tctttcagcg
    15841 ccgcgttaac gatatctacc ggcttctgac caacatcaga agggaagaaa tcaacgccca
    15901 cctgctctgc cagcgtctca agctgtttga ttgccgccgg gcgataaacg tcggcagaaa
    15961 ccaccagcac tttcttcttg tgcttctcgc gcaggaactt accgagttta ccaacgctgg
    16021 ttgttttacc ggcaccttgc aggcccgcca tcagcacgac cgcaggcggt tgcgccgcca
    16081 ggttcagggt ctggttctct tcgcccatcg ccgcaaccag ttcgttacgg acgattttga
    16141 cgaactcctg ccccggcgtc aggctcttat taacttcatg accaaccgct ttctctttta
    16201 cgcgattgat aaactcacgc actaccggca gagcgacgtc cgcctccagc agcgccatgc
    16261 gcacttcgcg cagcgtatct tttacgttgt cttcagtgag gcgtccacgg ccactgatat
    16321 tgcgcagcgt gcgcgacaaa cgatcggtta aattatcaaa cattgtctct cgcctggggt
    16381 ggaaacggtt ggccgcaatc gcgacacatc atcagtattt tgccgcagta taacatgaag
    16441 gcgtctttgt tgttatgcaa cggttggagc agcgttcacc tgacgctata ctgcttctct
    16501 ttcttattgc tcaaactgtc gacattacta tgcccgtttt tgctctgctc gcgcttgtcg
    16561 cctactccgt cagtcttgcg ctgattgttc ccggtctgct gcaaaaaaac ggcggctggc
    16621 ggcgcatggc tattatttct gcggtcattg cgctggtctg ccacgcaatc gctctggaag
    16681 cccgcatcct gcccgacggt gatagcggac aaaacctcag cctgctgaac gttggttcat
    16741 tggtcagttt gatgatctgt acggtaatga ccattgtggc ttctcgcaat cgtggctggt
    16801 tgctgctacc cattgtctat gcctttgcgc ttatcaacct ggcgctggcg accttcatgc
    16861 ccaatgaata catcacccat ctggaagcaa cgcctgggat gctggtgcac attggcttat
    16921 cgctcttttc ctatgccacg ctaattatcg ccgccctgta cgcgctgcaa ctggcgtgga
    16981 ttgattacca actgaagaac aagaagctgg catttaacca ggaaatgccg ccattgatga
    17041 gtatcgagcg taaaatgttc cacatcacgc agattggcgt ggtgctgcta acgctcacgc
    17101 tttgcactgg cctgttctac atgcacaacc tatttagcat ggaaaatatc gacaaggctg
    17161 tgctctctat cgtggcgtgg tttgtctata ttgtgctgct gtggggacat taccatgaag
    17221 gatggcgtgg acgccgcgtc gtctggttta acgttgcggg cgcggtaatt ctgacactgg
    17281 cctacttcgg cagccgaatt gtccagcagt taatcagcta aacccagaaa aggagtttcc
    17341 cctggaacac atttctacta ctacgttgat cattattctg atcatcatgg tggtcatttc
    17401 agcctatttt tccgggtccg aaaccggaat gatgaccctc aaccgctatc gtctgcgaca
    17461 tatggcgaaa cagggtaatc gctcggccaa acgcgtcgaa aaattgctgc gtaagccaga
    17521 ccgcctgata agcctggtgt taatcggcaa taacctggtc aatattcttg cctccgcgct
    17581 cggcactatt gttgggatgc gtttgtacgg cgatgcgggc gtggcaattg cgactggtgt
    17641 gctgactttt gtcgtactgg tatttgctga ggtattgccg aaaaccattg ccgcgctgta
    17701 cccggaaaaa gtcgcttatc cgagtagttt tctgctggct ccgctgcaaa ttttgatgat
    17761 gccgctggtc tggttgctga atgctatcac ccgtatgctg atgcgcatga tgggtatcaa
    17821 aaccgatatc gtggttagcg gctctttgag caaagaagag ttgcgcacta tcgtgcacga
    17881 atcacgctca caaatttccc gtcgcaatca ggatatgctg ctgtcggtgc tcgatctgga
    17941 aaaaatgacc gttgatgaca tcatggtgcc gcgcagtgaa attatcggta ttgatatcaa
    18001 cgatgactgg aaatcgattc tgcgccaact ctcccactca cctcacgggc gcatcgtgct
    18061 ctaccgtgat tcgctggacg acgccatcag tatgctgcga gtacgtgaag cctggcggtt
    18121 gatgtcggag aaaaaagagt tcaccaaaga aaccatgctg cgcgccgcgg acgagatcta
    18181 ttttgtgccg gaaggtacgc cgctcagcac gcagttggta aagtttcagc gcaacaaaaa
    18241 gaaagtaggc ctggtcgtca acgagtatgg agacattcag gggctggtga cggttgaaga
    18301 tattctggaa gagattgtcg gcgatttcac tacgtcgatg tcgccaacac ttgccgaaga
    18361 ggtcacgccg caaaacgacg gttcggtgat tatcgatggc accgccaacg tgcgggaaat
    18421 caacaaagcc tttaactggc atctaccgga agatgatgcc cgcacggtta atggcgtcat
    18481 tcttgaggca ctggaagaga tccctgtcgc aggcacccgc gtgcgtattg gcgagtacga
    18541 tatagatatt ctcgacgtac aggacaatat gattaagcag gtaaaagttt ttcctgtgaa
    18601 accgctgcgc gagagtgtgg cggagtaacg aaaacggccc tgcattcgca tgcagggccg
    18661 tgaattatta cgaaaacaga aattacgctt ttgctttcgc tacagttacc atcgccgcac
    18721 gaatcgtacg accattcagc gtataaccct tctgcataat gcccagtacg ttacctggcg
    18781 caacgtcatc agattccacc attgcgatag cctgatgcac attcgggtcc agcggcacgt
    18841 tagtttcggc gatcacttca acaccaaact tacgcacaac atccagcatc gacttcagcg
    18901 tcagctcaat gccttcaacc atcgcggaca tatccgggtt agctttatca gccacttcca
    18961 gcgcacgatc caggctatca atcaccggca gcaattcgtt gatgaatttc tccagcgcga
    19021 atttgtgggc tttttcaata tccagttcag tacgtcgacg caggttttcc atttcggctt
    19081 ttatacgcaa aatgccgtca cgttcacggg tctgggcttc agccagctga gcttcgagat
    19141 tcgcaatttt ttcatcgcgc gggtccacct gctcagcaga agcttctggc tcaactgcct
    19201 caatctcttc gtgctgatcc atgataattt cttccggggc ttgcccctca ggcgttttct
    19261 gttctttact actcatgaat ttctccgcgt ttttttcgca ttcatctcgc taacttcgct
    19321 tattatgggg atcagtttca gggtttcaag ggaagcactc acattgtcat caatcttcgc
    19381 aacaaggacc tcggaaaaat gaataatcat ttcaagtgta ttggcattgt gggacaccca
    19441 cggcacccca ctgcactgac aacacatgaa atgctctacc gctggctgtg cacaaaaggt
    19501 tacgaggtca tcgttgagca acaaatcgct cacgaactgc aactgaagaa tgtgaaaact
    19561 ggcacgctcg cggagattgg gcaactagct gatctcgcgg tagtcgttgg tggcgacggt
    19621 aatatgctgg gcgcggcacg cacactcgcc cgttacgata ttaaagttat tggaatcaac
    19681 cgtggcaacc tgggtttcct gactgacctt gaccccgata acgcccagca acagttagcc
    19741 gatgtgctgg aaggccacta catcagcgag aaacgttttt tgctggaagc gcaagtctgt
    19801 cagcaagatt gccagaaacg catcagcacc gcgataaatg aagtggtgct tcatccaggc
    19861 aaagtggcgc atatgattga gttcgaagtg tatatcgacg agatctttgc gttttctcag
    19921 cgatctgatg gactaattat ttcgacgcca acaggctcca ccgcctattc cctctctgca
    19981 ggcggtccta ttctgacccc ctctctggat gcgattaccc tggtgcccat gttcccgcat
    20041 acgttgtcag cacgaccact ggtcataaac agcagcagca cgatccgtct gcgtttttcg
    20101 catcgccgta acgacctgga aatcagttgc gacagccaga tagcactgcc gattcaggaa
    20161 ggtgaagatg tcctgattcg tcgctgtgat taccatctga atctgattca tccgaaagat
    20221 tacagttatt tcaacacatt aagcaccaag ctcggctggt caaaaaaatt attctaattt
    20281 tacgccagcc tctttactgt atataaaacc agtttatact gtacacaata acagtaatgg
    20341 tttttcatac aggaaaacga ctatgttggc acaactgacc atcagcaact ttgctatcgt
    20401 tcgtgagctt gagattgatt ttcatagcgg catgaccgta ataactggcg agaccggcgc
    20461 gggtaaatct attgcaatag atgccctcgg tctttgtctc ggtggtcgcg ctgaagccga
    20521 catggtgcgt accggcgctg ctcgcgctga cctgtgcgcc cgtttttctc tgaaagatac
    20581 gccagcggct ctgcgctggc tggaagaaaa ccagcttgaa gacgggcatg aatgtttgct
    20641 tcgtcgcgtg atcagcagcg atggtcgctc ccgtggtttc atcaacggta cagctgttcc
    20701 tctgtcacaa ctgcgcgaac tgggtcagtt gctgattcag atccatggtc agcacgctca
    20761 tcagttactc accaaacccg agcaccaaaa gttcctgctt gatggctatg ccaatgaaac
    20821 ctctctactc caggaaatga cagcacgtta tcagttgtgg catcaaagct gccgtgacct
    20881 cgcgcatcat caacagttaa gtcaggaacg cgctgcccgt gcggaactgc tgcaatacca
    20941 attaaaagaa cttaacgaat ttaatccgca gcccggagag tttgaacaaa tcgacgaaga
    21001 gtacaaacgt ctggcgaaca gcggtcaatt gctgaccacc agccagaatg cattggcatt
    21061 aatggccgac ggtgaagacg caaacctgca aagtcagctt tacacggcta aacaactggt
    21121 gagcgaattg attggcatgg acagcaaact gtccggcgta cttgatatgc tggaagaagc
    21181 taccatccag attgctgaag ccagcgatga actgcgccac tactgcgatc gtctggatct
    21241 cgatcccaac cgactatttg aacttgaaca gcgcatctca aaacagattt cgctggcacg
    21301 taaacatcac gtcagccctg aggcattgcc acagtattac cagtcgctac tggaagaaca
    21361 gcagcaactg gacgatcagg ccgactcaca agaaacgctt gcgctggcgg taacgaaaca
    21421 tcatcagcag gcactggaaa tcgcgcgcgc attacaccaa caacgccagc aatatgcaga
    21481 agaacttgca cagctgatca ccgacagtat gcatgcgctc tcaatgccgc atgggcagtt
    21541 tacgatcgat gttaaatttg acgagcatca cctgggcgct gacggtgccg atcgtattga
    21601 gtttcgggta accaccaacc caggtcagcc aatgcagcct attgccaaag tcgcatccgg
    21661 tggtgaattg tcccgcatcg cactggcaat ccaggtcatc acggcgcgta aaatggaaac
    21721 cccggcactg atttttgatg aagtggatgt agggattagc ggtccaacag cggcagttgt
    21781 cggcaaactg ctgcgtcaac tcggcgaatc aactcaggtg atgtgtgtta cccacctgcc
    21841 acaagtcgcg ggatgtggtc atcaacacta ttttgtcagc aaagaaaccg atggtgcgat
    21901 gacagaaacg catatgcaat ccctgaataa aaaagcgcgg ttacaagagc tggcgcgcct
    21961 gcttggtggc agtgaagtca cacgtaatac actggcgaat gcgaaagaac tgcttgcagc
    22021 gtaaactttt ttcctgcttc acggtcagag taaacagcaa aacgccgtaa gaccggaaag
    22081 caaaaggttt taaagtgatg aaaggtctat tatcatcggc atattacaga tgagccacgt
    22141 actgctcggg cccgaaaagg aatcaaatca ctatgcgctg taaaacgctg actgctgcag
    22201 cagcagtact attgatgttg accgcaggct gttccactct ggagcgagtg gtttaccgtc
    22261 ctgacatcaa ccaggggaac tatctgaccg ctaacgacgt atccaaaata cgtgttggca
    22321 tgacgcaaca acaagttgcg tacgcattgg gtacaccgct gatgtccgat ccatttggta
    22381 cgaatacctg gttctatgtc ttccgccagc aaccaggtca tgaaggtgta acgcagcaaa
    22441 cgctgacgct gacctttaac agtagcggtg tgttgaccaa tattgataac aaacctgcgc
    22501 tgagtggtaa ctaataataa agttgctctc aaagacgtta aaaaaggtgc tcaatgagca
    22561 ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc gaagctcttt
    22621 cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca cactatcgct
    22681 tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga tatcggtacg
    22741 caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct cctgcagcgt
    22801 cactcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg caattttacc
    22861 tggcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta gccgccagct
    22921 ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca aagtcgagat
    22981 gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca ccaatcaatt
    23041 tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc aactggttgc
    23101 gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca gtcatctgcc
    23161 caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga ggataagact
    23221 gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt gcggtccggc
    23281 taatctgagg cataacaatt tccatcaaca aaaatccaac aaataatatc atttatcctg
    23341 ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa gatagagcct
    23401 tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg attcacgacg
    23461 cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct taacaagcgc
    23521 gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct gcaaggctgg
    23581 gaggttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt ccttctgcgt
    23641 gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc ctccacgcat
    23701 gtggtgtgcg atcctacccg tacccgcaag ttacttctca accaacgcga actggactca
    23761 ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct gtactggaaa
    23821 aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca cgataaacgt
    23881 tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa aaacgcccac
    23941 cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc tccggcggcg
    24001 cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc attgcttaat
    24061 cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat tgaggctggt
    24121 catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt ctggattcga
    24181 cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa aaagccgcaa
    24241 aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc ttagagccct
    24301 ctctccctag cctccgctct taggacgggg atcaagagag ctcaaaccca aaagagatcg
    24361 cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt tgttagtggc
    24421 gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag taccgaggat
    24481 gtaggaattt cggacgcggg ttcaactccc gccagctcca ccaaatattg atgtactgaa
    24541 gttcagtaaa gtctactaag cccgcacagc acaagctctg cgggcttttt tacgtctatt
    24601 gtagtctagt gagaattgct gagaactacg agttatggca ccctgaatgg gacccacgaa
    24661 gatgggtcct aaaatcgagg gtcccaaaca tggccaaaat cgctaagaag ctcactgaca
    24721 ctgaaatcaa aagcaccaaa cctgccgaaa aagaggttaa cctttttgac ggcgatggtt
    24781 tgctgttgcg aatcgctccc ctggcgaagg gagggaagaa aaattggtat ttcagatatg
    24841 cagtgcctgt gaccaaaaag cgaactaagg tgagcttagg aacctatcct caccttacac
    24901 ttgcgaaggc acgagcttta cgtgatgaat acttgtcgtt gcttacaaat ggtatagacc
    24961 cccaagttca taacaaccaa aaagccaatg cactgaaaga tgccacggaa catacatttc
    25021 aagcagtagc caagaagtgg cttgatgaga aagtcaaaac gtcaggcatc tcccaagatc
    25081 atgctaacga catctggcga agcctagaga gaaatatctt ttccacattg ggtgataccc
    25141 caattaagga gattcgccct aaaatgctta aacagcattt agaacccata gaaaaacgag
    25201 gtgtccttga aacacttcgc cgcatcatat cccgcctgaa tgaaattttc cgctatgcag
    25261 caacagaaga actcatagaa ttcaacccgg cagacaacct ggggcaacgg ttcagcaagc
    25321 caaaaaaaca gaatatgcca gcattacccc cttccgaact ccctcgcttc ttggttgctc
    25381 taaacaatgc ttctatccgt ttggaaacaa ggctactgat tgagtggcaa cttctcacat
    25441 gggttcgccc aggtgaagct gttcgcacaa gatggtcaga tattgatatt gaaactggca
    25501 tgtggaacat cccggcggag tttatgaaaa tgaagaagcc tcacaaagtt ccactgagca
    25561 aagaagcttt gcgagttttg gatttaatga aagtcatcag cgggcataga gagtgggtgt
    25621 tccccagtat caaagctcca ctcaatcaca tgcatgaaca aacagctaat gcggccataa
    25681 tccgtatggg tttcggaggt gagcttgtag ctcacggtat gcgatccatt gctagaacgg
    25741 ctgctgagga gtctggcaag tttaggactg atgtcttaga agccgccctt gcccactcga
    25801 agaaagatga aataattgca gcctacaatc gtgcagagta tctcactgaa cgggtggttc
    25861 tcatgcaatg gtggagtgac tatgtttcgt ctcaaaaatg caaagttatt gccgcataac
    25921 tctcccatga tggattaact atcttgattt agttaaagaa ttaataatca caccattaac
    25981 ctatgtggac taagcatagc catttacaaa tggggacatt gagtccacat aacgaaagct
    26041 gtcggtcata tcagctaaat aattcacatc tcttctcgat tatcatacac ctcgaagatc
    26101 taccaaattc gctctaatca atgataaaac agttgaattc ggttgaaatt tgatcaattt
    26161 tatccactac attgtattga atcatccatg aggtttcgtg catggctagc gaaaatgaca
    26221 aaaatcatag agttagggtc gcgcagtact tgaggatgtc taccgaccat cagcaatatt
    26281 ctttacataa tcagtccgaa tatatcaaag attatgctga aaagaacaat atggaaatcg
    26341 cttataccta cgatgatgca ggtaagagcg gagtcagtat cgtaggcagg cattctttgc
    26401 agcagttact tagcgatgta gaacaaaaga aaatagatat acaggctgta ttattttatg
    26461 atgtgagccg ttttggtcgt tttcaaaata gtgatgaagc ggcatattat tcctttctat
    26521 ttgagagaaa tggtgtagat cttatatatt gttccgaacc tatacccact aaagatttcc
    26581 ctttagagtc ctctgttata ctgaatataa aaagatctag tgctgcatat cacagcagga
    26641 atctatctga aaaggtattt atagggcaag taaatttaat aaagcttggt tatcatcaag
    26701 gcggtatggc tggttatggg ctgagacgtc ttttagtaga cgaaaatggc atagctaaag
    26761 aa
//
LOCUS       DAFNBJ010000058        26671 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000058 DAFNBJ010000000
VERSION     DAFNBJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.058"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            23..1423
                     /gene="gspA"
                     /locus_tag="L2X49_002266"
     CDS             23..1423
                     /gene="gspA"
                     /locus_tag="L2X49_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HBN4797347.1"
                     /translation="MLGEAGSGKTWLAQQLQKDKHRRVITLSLVVSWQGKAAWIVTDD
                     NAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLIIIENAHLNHRRILDDLQRAISLI
                     PDGQFLLIGRPDRKVERDFKKQGIELVSIGRLTEHELKASILEGQNIDQPDLLLTARV
                     LKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFTAKQWRMIYRILGDNRPRKMQLAV
                     VMSGTIIALTCGWLLLSSFTATLPVPAWLIPVTPVVKQDMTKDIAHVVMRDSEALSVL
                     YGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTLVDQNLPWIASLKVGDKKLPVVVV
                     RVGEASVDVLVGQQTWTLTHKWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLE
                     TMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYL
                     YRDEANISPETTVKGK"
     gene            1425..1844
                     /gene="gspB"
                     /locus_tag="L2X49_002267"
     CDS             1425..1844
                     /gene="gspB"
                     /locus_tag="L2X49_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HBN4797348.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2082..2393
                     /gene="rpsJ"
                     /locus_tag="L2X49_002268"
     CDS             2082..2393
                     /gene="rpsJ"
                     /locus_tag="L2X49_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBN4797349.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2426..3055
                     /gene="rplC"
                     /locus_tag="L2X49_002269"
     CDS             2426..3055
                     /gene="rplC"
                     /locus_tag="L2X49_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBN4797350.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3066..3671
                     /gene="rplD"
                     /locus_tag="L2X49_002270"
     CDS             3066..3671
                     /gene="rplD"
                     /locus_tag="L2X49_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBN4797351.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3668..3970
                     /gene="rplW"
                     /locus_tag="L2X49_002271"
     CDS             3668..3970
                     /gene="rplW"
                     /locus_tag="L2X49_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBN4797352.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3988..4809
                     /gene="rplB"
                     /locus_tag="L2X49_002272"
     CDS             3988..4809
                     /gene="rplB"
                     /locus_tag="L2X49_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBN4797353.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4826..5104
                     /gene="rpsS"
                     /locus_tag="L2X49_002273"
     CDS             4826..5104
                     /gene="rpsS"
                     /locus_tag="L2X49_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBN4797354.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5119..5451
                     /gene="rplV"
                     /locus_tag="L2X49_002274"
     CDS             5119..5451
                     /gene="rplV"
                     /locus_tag="L2X49_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBN4797355.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5469..6170
                     /gene="rpsC"
                     /locus_tag="L2X49_002275"
     CDS             5469..6170
                     /gene="rpsC"
                     /locus_tag="L2X49_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBN4797356.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6183..6593
                     /gene="rplP"
                     /locus_tag="L2X49_002276"
     CDS             6183..6593
                     /gene="rplP"
                     /locus_tag="L2X49_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBN4797357.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6593..6784
                     /gene="rpmC"
                     /locus_tag="L2X49_002277"
     CDS             6593..6784
                     /gene="rpmC"
                     /locus_tag="L2X49_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBN4797358.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6784..7038
                     /gene="rpsQ"
                     /locus_tag="L2X49_002278"
     CDS             6784..7038
                     /gene="rpsQ"
                     /locus_tag="L2X49_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBN4797359.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7203..7574
                     /gene="rplN"
                     /locus_tag="L2X49_002279"
     CDS             7203..7574
                     /gene="rplN"
                     /locus_tag="L2X49_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBN4797360.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7585..7899
                     /gene="rplX"
                     /locus_tag="L2X49_002280"
     CDS             7585..7899
                     /gene="rplX"
                     /locus_tag="L2X49_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBN4797361.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7914..8453
                     /gene="rplE"
                     /locus_tag="L2X49_002281"
     CDS             7914..8453
                     /gene="rplE"
                     /locus_tag="L2X49_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBN4797362.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8468..8773
                     /gene="rpsN"
                     /locus_tag="L2X49_002282"
     CDS             8468..8773
                     /gene="rpsN"
                     /locus_tag="L2X49_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBN4797363.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8807..9199
                     /gene="rpsH"
                     /locus_tag="L2X49_002283"
     CDS             8807..9199
                     /gene="rpsH"
                     /locus_tag="L2X49_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBN4797364.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9212..9745
                     /gene="rplF"
                     /locus_tag="L2X49_002284"
     CDS             9212..9745
                     /gene="rplF"
                     /locus_tag="L2X49_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBN4797365.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9755..10108
                     /gene="rplR"
                     /locus_tag="L2X49_002285"
     CDS             9755..10108
                     /gene="rplR"
                     /locus_tag="L2X49_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBN4797366.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10123..10626
                     /gene="rpsE"
                     /locus_tag="L2X49_002286"
     CDS             10123..10626
                     /gene="rpsE"
                     /locus_tag="L2X49_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBN4797367.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10630..10809
                     /gene="rpmD"
                     /locus_tag="L2X49_002287"
     CDS             10630..10809
                     /gene="rpmD"
                     /locus_tag="L2X49_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBN4797368.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10813..11247
                     /gene="rplO"
                     /locus_tag="L2X49_002288"
     CDS             10813..11247
                     /gene="rplO"
                     /locus_tag="L2X49_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBN4797369.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11255..12586
                     /gene="secY"
                     /locus_tag="L2X49_002289"
     CDS             11255..12586
                     /gene="secY"
                     /locus_tag="L2X49_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBN4797370.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12618..12734
                     /gene="rpmJ"
                     /locus_tag="L2X49_002290"
     CDS             12618..12734
                     /gene="rpmJ"
                     /locus_tag="L2X49_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBN4797371.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12881..13237
                     /gene="rpsM"
                     /locus_tag="L2X49_002291"
     CDS             12881..13237
                     /gene="rpsM"
                     /locus_tag="L2X49_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBN4797372.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13254..13643
                     /gene="rpsK"
                     /locus_tag="L2X49_002292"
     CDS             13254..13643
                     /gene="rpsK"
                     /locus_tag="L2X49_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBN4797373.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13677..14297
                     /gene="rpsD"
                     /locus_tag="L2X49_002293"
     CDS             13677..14297
                     /gene="rpsD"
                     /locus_tag="L2X49_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBN4797374.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14323..15312
                     /gene="rpoA"
                     /locus_tag="L2X49_002294"
     CDS             14323..15312
                     /gene="rpoA"
                     /locus_tag="L2X49_002294"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBN4797375.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15353..15736
                     /gene="rplQ"
                     /locus_tag="L2X49_002295"
     CDS             15353..15736
                     /gene="rplQ"
                     /locus_tag="L2X49_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBN4797376.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15843..16211
                     /locus_tag="L2X49_002296"
     CDS             15843..16211
                     /locus_tag="L2X49_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HBN4797377.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16222..16647
                     /gene="zntR"
                     /locus_tag="L2X49_002297"
     CDS             16222..16647
                     /gene="zntR"
                     /locus_tag="L2X49_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HBN4797378.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16703..16921
                     /gene="arfA"
                     /locus_tag="L2X49_002298"
     CDS             16703..16921
                     /gene="arfA"
                     /locus_tag="L2X49_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HBN4797379.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16918..17328)
                     /gene="mscL"
                     /locus_tag="L2X49_002299"
     CDS             complement(16918..17328)
                     /gene="mscL"
                     /locus_tag="L2X49_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBN4797380.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17458..18834)
                     /gene="trkA"
                     /locus_tag="L2X49_002300"
     CDS             complement(17458..18834)
                     /gene="trkA"
                     /locus_tag="L2X49_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBN4797381.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18856..20145)
                     /gene="rsmB"
                     /locus_tag="L2X49_002301"
     CDS             complement(18856..20145)
                     /gene="rsmB"
                     /locus_tag="L2X49_002301"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBN4797382.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(20191..21138)
                     /gene="fmt"
                     /locus_tag="L2X49_002302"
     CDS             complement(20191..21138)
                     /gene="fmt"
                     /locus_tag="L2X49_002302"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBN4797383.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGMTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21153..21662)
                     /gene="def"
                     /locus_tag="L2X49_002303"
     CDS             complement(21153..21662)
                     /gene="def"
                     /locus_tag="L2X49_002303"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBN4797384.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21792..22916
                     /gene="dprA"
                     /locus_tag="L2X49_002304"
     CDS             21792..22916
                     /gene="dprA"
                     /locus_tag="L2X49_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBN4797385.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22888..23361
                     /locus_tag="L2X49_002305"
     CDS             22888..23361
                     /locus_tag="L2X49_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBN4797386.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23390..23932
                     /locus_tag="L2X49_002306"
     CDS             23390..23932
                     /locus_tag="L2X49_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBN4797387.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23937..24509
                     /gene="tsaC"
                     /locus_tag="L2X49_002307"
     CDS             23937..24509
                     /gene="tsaC"
                     /locus_tag="L2X49_002307"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBN4797388.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24514..25332
                     /gene="aroE"
                     /locus_tag="L2X49_002308"
     CDS             24514..25332
                     /gene="aroE"
                     /locus_tag="L2X49_002308"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBN4797389.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALSMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKCNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25329..25586
                     /locus_tag="L2X49_002309"
     CDS             25329..25586
                     /locus_tag="L2X49_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBN4797390.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25562..26116)
                     /locus_tag="L2X49_002310"
     CDS             complement(25562..26116)
                     /locus_tag="L2X49_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBN4797391.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            26589..>26671
                     /locus_tag="L2X49_002311"
     rRNA            26589..>26671
                     /locus_tag="L2X49_002311"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6819 a         6304 c         6971 g         6577 t
ORIGIN      
        1 tcaaacctgg cgtctatgct atttgttggg tgaggctgga agtggaaaaa cctggctggc
       61 gcagcaactg caaaaagata aacatcgccg tgtgattact ttaagcctcg ttgtttcctg
      121 gcaaggtaag gccgcatgga tcgttaccga cgataacgcg gctgaacagg gctgccgtga
      181 cagcgcctgg acgcgagatg agatggcggg gcaattactg catgcgcttc atcgaactga
      241 tagtcgctgc cctcttatta ttattgaaaa tgctcacctg aatcaccgcc gcatacttga
      301 tgatctccaa cgcgcaatat ctcttattcc tgacggtcaa tttttgctga tcggcagacc
      361 cgatcgaaaa gtcgaacgtg attttaaaaa acagggcatt gaacttgtct cgataggacg
      421 tctgacggag cacgaactta aggcaagcat ccttgaaggt cagaatattg accaacccga
      481 tctcctttta accgccagag ttctgaaacg gatagcttta ttatgtcggg gcgatcgcag
      541 aaagctggcg cttgccggcg aaacaattcg cttattacag caagccgagc agaccagcgt
      601 tttcacagca aaacaatggc gaatgattta ccgcatattg ggtgataacc gcccccggaa
      661 aatgcagctt gccgtggtga tgagtggaac cattatcgct cttacctgtg gttggctact
      721 gctgtcctct tttacagcta cgttacccgt tcctgcgtgg ctgatacccg taacgccagt
      781 agttaaacaa gatatgacta aggatattgc tcatgtggtg atgcgcgata gtgaggcgct
      841 aagcgtgtta tacggcgtat gggggtacga ggtcccagct gattcggcct ggtgcgatca
      901 ggcagtaaga gcggggctgg catgtaaaag tgggaatgct tctttacaaa cattggtaga
      961 tcagaatttg ccctggatcg catcgcttaa agtcggggac aaaaaattgc ctgttgtggt
     1021 cgttcgcgtc ggtgaggcta gcgttgatgt cctcgtaggt cagcaaacgt ggacgctgac
     1081 acataaatgg tttgagtcgg tatggacggg tgattatctt ctgttgtgga aaatgtcgcc
     1141 ggaaggggag agtaccatta cgcgtgatag cagcgaggaa gagattctct ggctggaaac
     1201 gatgttaaac cgcgcattgc atatttcgac agaaccttcg gcggagtggc gtcccttgtt
     1261 ggtagaaaaa ataaagcaat ttcaaaaaag ccaccacttg aaaactgacg gtgttgttgg
     1321 cttttcaacg ttagtacatc tctggcaggt ggcaggggag agtgcctact tatatcggga
     1381 tgaagcaaat atttccccgg aaacaacagt gaaggggaaa tgacatgttt gaattctata
     1441 tcgccgcgcg tgagcagaaa gaaacaggtc accctggtat cttctcccga caaaaacaca
     1501 gcaccataat atatgtaatt tgtttattac tgatttgcct gtggtttgca ggaatggtgc
     1561 tggttggtgg gtatgccagg cagctatggg tgctttggat agtaaaagcc gaagtcactg
     1621 tagaggctga aacgcccgct tttaaacaat caacacaaca ctatttcttc aaaaaacagc
     1681 cactccccgt cgtcgaatcc gtcgaggagg aagatgaccc agacgtcgcg gttgagaatg
     1741 ccccctcttc ttctgaagac gaagaaaata ctgtggaaga aagcgacgag aaagccggtt
     1801 taagagagcg tgtcaaaaat gcactgaacg aacttgagag ataacccgaa ggctgtttac
     1861 tttactagca atacgcttgc gttcggtggt taagtatgta taatgcgcgg gcttgtcgta
     1921 gttgacagca ggttcaatct gaacctcagt tagccgaatt tggctaccta acaatgctcc
     1981 caatcgggga gctacgtaag aacggttaca ctctcccatc aatcgtaatg ggtctgagga
     2041 gtaatcattt tcgtttataa aataattgga gctctggtct catgcagaac caaagaatcc
     2101 gtatccgcct gaaagcgttt gatcatcgtc tgatcgatca agcaaccgcg gaaatcgtcg
     2161 agactgccaa gcgcactggt gcgcaggtcc gtggtccgat cccgctgccg acacgcaaag
     2221 agcgcttcac tgttctgatc tccccgcacg tcaacaaaga cgcgcgcgat cagtacgaaa
     2281 tccgtactca cttgcgtctg gttgacatcg ttgagccaac cgagaaaacc gttgatgctc
     2341 tgatgcgtct ggatctggct gccggtgtag acgtgcagat cagcctgggt taatcaggtc
     2401 attgagcgat tgagaggttg aaacaatgat tggtttagtc ggtaaaaaag tgggtatgac
     2461 ccgtatcttc acagaagacg gcgtttctat cccagtaacc gtaatcgaag ttgaagcaaa
     2521 ccgcgttact caggttaaag acctggctaa cgatggctac cgtgctattc aggtgaccac
     2581 cggtgctaaa aaagctaacc gtgtgaccaa gcctgaagct ggccacttcg ctaaagctgg
     2641 cgtagaagct ggccgtggtc tgtgggaatt ccgcctggct gaaggcgaag agttcactgt
     2701 aggtcagagc attagcgttg aactgtttgc tgacgttaaa aaagttgacg taactggcac
     2761 ctctaaaggt aaaggtttcg caggtaccgt taagcgctgg aacttccgta cccaggacgc
     2821 tactcacggt aactccttgt ctcaccgcgt tccgggttct atcggtcaga accagactcc
     2881 gggcaaagtg ttcaaaggca agaaaatggc aggtcagatg ggtaacgaac gtgtaaccgt
     2941 tcagagcctt gacgtagtac gcgttgacgc tgagcgcaac ctgctgctgg ttaaaggtgc
     3001 tgtcccgggt gcaaccggta gcgacctgat cgttaaacca gctgtgaagg cgtaaggaga
     3061 tagcaatgga attagtattg aaagacgcgc agagcgcgct gactgtttcc gaaactacct
     3121 tcggtcgtga tttcaacgaa gcgctggttc accaggttgt tgttgcttat gcagctggtg
     3181 ctcgtcaggg tactcgtgct cagaagactc gtgctgaagt aactggttcc ggtaaaaaac
     3241 cgtggcgcca gaaaggcacc ggccgtgcgc gttctggttc tatcaagagc ccgatctggc
     3301 gttctggtgg cgtgaccttt gctgctcgtc cgcaggacca cagtcaaaaa gttaacaaga
     3361 agatgtaccg cggcgcgctg aaaagcatcc tgtccgaact ggtacgtcag gatcgtctga
     3421 tcgttgtcga gaagttctct gtagaagcgc cgaaaactaa gctgctggca cagaaactga
     3481 aagacatggc tctggaagat gtgctgatca tcaccggtga gctggacgaa aacctgttcc
     3541 tggctgcgcg caacctgcac aaggttgacg tacgcgatgc aactggtatc gacccggtta
     3601 gcctgatcgc cttcgacaaa gtcgtaatga ctgctgatgc tgttaagcaa gttgaggaga
     3661 tgctggcatg attcgtgaag aacgtctgct gaaggtgctg cgtgcaccgc acgtttctga
     3721 aaaagcgtct actgcgatgg aaaaatccaa caccatcgta ctcaaagttg ctaaagacgc
     3781 gaccaaagca gaaatcaaag ctgctgtgca gaaactgttt gaagtcgaag tcgaagtcgt
     3841 taacaccctg gtagttaaag ggaaagttaa acgtcacgga cagcgtatcg gtcgtcgtag
     3901 cgactggaaa aaagcttacg tcaccctgaa agaaggccag aatctggact tcgttggcgg
     3961 cgctgagtaa gtcggaggag taatacaatg gcagttgtta aatgtaaacc gacatctccg
     4021 ggtcgtcgcc acgtagttaa agtggttaac cctgagctgc acaagggcaa accttttgct
     4081 ccgttgctgg aaaaaaacag caaatccggt ggtcgtaaca acaatggccg tatcaccact
     4141 cgtcatatcg gtggtggcca caagcaggct taccgtattg ttgacttcaa acgcaacaaa
     4201 gacggtatcc cggcagttgt tgaacgtctt gagtacgatc cgaaccgttc cgcgaacatc
     4261 gcgctggttc tgtacaaaga cggtgaacgc cgttacatcc tggcccctaa aggcctgaaa
     4321 gctggcgacc agattcagtc tggcgttgat gctgcaatca aaccaggtaa caccctgccg
     4381 atgcgcaaca tcccggttgg ttctactgtt cataacgtag aaatgaaacc aggtaaaggc
     4441 ggtcagctgg cacgttccgc tggtacttac gttcagatcg ttgctcgtga tggtgcttat
     4501 gtcaccctgc gtctgcgttc tggtgaaatg cgtaaagtag aagcagactg ccgtgcaact
     4561 ctgggcgaag ttggcaatgc tgagcatatg ctgcgcgttc tgggtaaagc aggtgctgca
     4621 cgctggcgtg gtgttcgtcc gaccgttcgc ggtaccgcga tgaacccggt agaccaccca
     4681 catggtggtg gtgaaggtcg taactttggt aagcacccgg taactccgtg gggcgttcag
     4741 accaaaggta agaagacccg cagcaacaag cgtactgata aattcatcgt acgtcgccgt
     4801 agcaaataat tttagaggat aagccatgcc acgttctctc aagaaaggtc cttttattga
     4861 cctgcacttg ctgaagaagg tagagaaagc ggtggaaagc ggagacaaga agcccctgcg
     4921 cacttggtcc cgtcgttcaa cgatctttcc taacatgatc ggtttgacca tcgctgtcca
     4981 taatggtcgt cagcacgttc cggtatttgt aaccgacgaa atggtcggtc acaaactggg
     5041 tgaattcgca ccgactcgta cttatcgcgg ccacgctgct gataaaaaag cgaagaagaa
     5101 ataaggtagg aggaagagat ggaaactatc gctaaacatc gccatgctcg ttcttctgct
     5161 cagaaggttc gccttgttgc tgacctgatt cgcggtaaga aagtgtcgca ggctctggat
     5221 attttgacct acaccaacaa gaaagcggct gtactggtca agaaagttct ggaatctgcc
     5281 attgctaacg ctgaacacaa cgatggcgct gacattgacg atctgaaagt tacgaaaatt
     5341 ttcgtagacg aaggcccgag catgaagcgc attatgccgc gtgcaaaagg tcgtgcagat
     5401 cgcatcctga agcgcaccag ccacatcact gtggttgtgt ccgatcgctg agactctgga
     5461 gactagcaat gggtcagaaa gtacatccta atggtattcg cctgggtatt gtaaaaccat
     5521 ggaactctac ctggtttgcg aacaccaaag aattcgctga caacctggac agcgatttta
     5581 aagtacgtca gtacctgact aaggaactgg ctaaagcgtc cgtatctcgt atcgttatcg
     5641 agcgtccggc taagagcatc cgtgtaacca ttcacactgc tcgcccgggt atcgttatcg
     5701 gtaaaaaagg tgaagacgta gaaaaactgc gtaaggtcgt agcggacatc gctggcgttc
     5761 ctgcacagat caacatcgcc gaagttcgta agcctgaact ggacgcaaaa ctggttgctg
     5821 acagcatcac ttctcagctg gaacgtcgcg ttatgttccg tcgtgctatg aagcgtgctg
     5881 tacagaacgc aatgcgtctg ggcgctaaag gtattaaagt tgaagttagc ggccgtctgg
     5941 gcggcgcgga aatcgcacgt accgaatggt accgcgaagg tcgcgtaccg ctgcacactc
     6001 tgcgtgctga catcgactac aacacctctg aagcgcacac cacttacggt gtaatcggcg
     6061 ttaaagtgtg gatcttcaaa ggcgagatcc tgggtggtat ggctgctgtt gaacaaccgg
     6121 aaaaaccggc tgctcagcct aaaaagcagc agcgtaaagg ccgtaaataa ggagcgtcgc
     6181 tgatgttaca accaaagcgt acaaaattcc gtaaaatgca caaaggccgt aaccgcggtc
     6241 tggcgcaggg tacggatgtt agcttcggca gcttcggtct gaaagctgtt ggccgtggtc
     6301 gtctgactgc ccgtcagatc gaagcagcac gtcgtgctat gacccgtgca gttaagcgtc
     6361 aaggtaagat ctggatccgt gtgttcccgg acaaaccgat cactgaaaag ccgctggcag
     6421 tgcgtatggg taaaggtaaa ggtaacgtgg agtattgggt tgccttgatt cagccgggta
     6481 aagtcctgta tgaaatggac ggtgttccgg aagagctggc ccgtgaagca ttcaagctgg
     6541 cagcagcgaa actgccgatt aaaaccacct ttgtaactaa gacggtgatg taatgaaagc
     6601 aaaagagctg cgtgagaaga gcgttgaaga gctgaacacc gagctgctga acctgctgcg
     6661 tgagcagttc aacctgcgta tgcaggctgc aagtggccag ctgcaacagt ctcacctgtt
     6721 gaagcaagtg cgtcgcgatg tcgcacgcgt taagacttta ctgaacgaga aggcgggtgc
     6781 gtaatgaccg ataaaatccg tactctgcaa ggtcgcgttg ttagcgacaa aatggagaaa
     6841 tccattgttg ttgctatcga acgttttgtg aaacacccga tctacggtaa attcatcaag
     6901 cgtacgacca aactgcacgt acatgacgag aacaacgaat gcggtatcgg tgacgtggtt
     6961 gaaatccgcg aatgccgtcc gctgtccaag actaaatcct ggacgctggt tcgcgttgta
     7021 gagaaagcgg ttctgtaata cagtacactc tctcgatacg aataaacggc tcagaaatga
     7081 gccgtttatt ttttctaccc atatccttga agcggtgtta taatgccgcg ccctcgatat
     7141 ggggattttt aacgacctga ttttcgggtc tcagtagtag ttgacattag cggagcacta
     7201 aaatgatcca agaacagact atgctgaacg tcgccgacaa ctccggtgca cgtcgcgtaa
     7261 tgtgtatcaa ggttctgggt ggctcgcacc gtcgctacgc aggcgtaggc gacatcatca
     7321 agatcaccat caaagaagca attccgcgtg gtaaggtcaa aaaaggtgat gtgctgaagg
     7381 cggtagtggt gcgcaccaag aagggtgttc gtcgcccgga cggttctgtc attcgcttcg
     7441 atggtaatgc ttgtgttctt ctgaacaaca acagcgagca gcctatcggt acgcgtattt
     7501 ttgggccggt aactcgtgag cttcgtagtg agaagttcat gaaaattatc tctctggcac
     7561 cagaagtact ctaaggagcg aatcatggca gcgaaaatcc gtcgtgatga cgaagttatc
     7621 gtgttaaccg gtaaagataa aggtaaacgc ggtaaagtta agaatgtcct gtcttccggc
     7681 aaggtcattg ttgaaggtat caacctggtt aagaaacatc agaagccggt tccggccctg
     7741 aaccaaccgg gtggcatcgt tgaaaaagaa gccgctattc aggtttccaa cgtagcaatc
     7801 ttcaatgcgg caaccggcaa ggctgaccgt gtaggcttta gattcgaaga cggcaaaaaa
     7861 gtccgtttct tcaagtctaa cagcgaaact atcaagtaat ttggagtagt acgatggcga
     7921 aactgcatga ttactacaaa gacgaagtag ttaaaaaact catgactgag tttaactaca
     7981 attctgtcat gcaagtccct cgggtcgaga agatcaccct gaacatgggt gttggtgaag
     8041 cgatcgctga caaaaaactg ctggataacg cagcagcaga cctagcagca atctccggtc
     8101 aaaaaccgct gatcaccaaa gcacgcaaat ctgttgcagg cttcaaaatc cgtcagggct
     8161 atccgatcgg ctgtaaagta actctgcgtg gcgaacgcat gtgggagttc tttgagcgcc
     8221 tgatcactat tgctgtacct cgtatccgtg acttccgtgg cctgtccgct aagtctttcg
     8281 acggtcgtgg taactacagc atgggtgtcc gtgagcagat catcttccca gaaatcgact
     8341 acgataaagt cgaccgcgtt cgtggtttgg atattaccat taccactact gcgaaatctg
     8401 acgaagaagg ccgcgctctg ctggctgcct ttgacttccc gttccgcaag taaggtaggg
     8461 ttactaaatg gctaagcaat caatgaaagc acgcgaagta aaacgcgtag ctttagctga
     8521 taaatacttc gcgaaacgcg ctgaactgaa agcgatcatc tctgatgtga acgcttccga
     8581 cgaagatcgt tggaacgctg ttctcaagct gcagactctg ccgcgtgatt ccagcccgtc
     8641 tcgtcagcgt aaccgctgcc gtcaaacagg tcgtccgcat ggtttcctgc ggaagttcgg
     8701 gttgagccgt attaaggtcc gtgaagccgc tatgcgcggt gaaatcccgg gtctgaaaaa
     8761 ggctagctgg taattgtcac caattgaatc acgggagtaa agacagatga gcatgcaaga
     8821 tccgatcgcg gatatgctga cccgtatccg taacggtcag gccgcgaaca aagctgcggt
     8881 caccatgcct tcctccaagc tgaaagtggc aatcgccaac gtgctgaagg aagaaggttt
     8941 tattgaagat tttaaagttg aaggcgacac caagcctgaa ctggaactta ctctgaagta
     9001 tttccagggc aaagctgttg tagaaagcat tcagcgtgtc agccgcccag gtctgcgcat
     9061 ctataaacgt aaagatgagc tgccgaaagt tatggcgggt ctgggtatcg cagttgtttc
     9121 tacctctaaa ggtgttatga ctgatcgtgc agcgcgccag gctggtcttg gtggcgaaat
     9181 tatctgctac gtagcctaat cggaggaaaa aatgtctcgt gttgctaaag caccggtcgt
     9241 tgttcctgcc ggcgttgacg taaaaatcaa cggtcaggtt attacgatca aaggtaaaaa
     9301 cggcgagctg actcgtactc tcaacgatgc tgttgaagtt aaacatgcag ataataccct
     9361 gaccttcggt ccgcgtgatg gttacgcaga cggttgggca caggctggta ccgcgcgtgc
     9421 cctgctgaac tcaatggtta tcggtgttac cgaaggcttc actaagaagc tgcagctggt
     9481 tggtgtaggt taccgtgcag cggttaaagg caatgtgatt aacctgtctc tgggtttctc
     9541 tcatcctgtt gaccatcagc tgcctgcggg tatcactgct gaatgtccga ctcagactga
     9601 aatcgtgctg aaaggcgctg ataagcaggt gatcggccag gttgcagcgg atctgcgcgc
     9661 ctaccgtcgt cctgagcctt ataaaggcaa gggtgttcgt tacgccgacg aagtcgtgcg
     9721 taccaaagag gctaagaaga agtaaggtaa cactatggat aagaaatctg ctcgtatccg
     9781 tcgtgcgacc cgcgcacgcc gcaagctcca ggagctgggc gcaactcgcc tggtggtaca
     9841 tcgtaccccg cgtcacattt acgcacaggt aattgcaccg aacggttctg aagttctggt
     9901 agctgcttct actgtagaaa aagctatcgc tgaacaactg aagtacaccg gtaacaaaga
     9961 cgcggctgca gctgtgggta aagctgtcgc tgaacgcgct ctggaaaaag gcatcaaaga
    10021 tgtatccttt gaccgttccg ggttccaata tcatggtcgt gtccaggcac tggcagatgc
    10081 tgcccgtgaa gctggccttc agttctaagg tagaggtgta agatggctca catcgaaaaa
    10141 caagctggcg aactgcagga aaagctgatc gcggtaaacc gcgtatctaa aaccgttaaa
    10201 ggtggtcgta ttttctcctt cacagctctg actgtagttg gcgatggtaa cggtcgcgtt
    10261 ggttttggtt acggtaaagc gcgtgaagtt ccagcagcga tccagaaagc gatggaaaaa
    10321 gcccgtcgca atatgattaa cgtcgcgctg aataacggca ctctgcaaca ccctgttaaa
    10381 ggtgttcaca cgggttctcg cgtattcatg cagccggctt ccgaaggtac cggtatcatc
    10441 gccggtggtg caatgcgcgc cgttctggaa gtcgctgggg ttcataacgt tctggctaaa
    10501 gcatatggtt ccaccaaccc gatcaacgtg gttcgtgcaa ctattgatgg cctggaaaat
    10561 atgaattctc cagaaatggt cgctgccaag cgtggtaaat ccgttgaaga aattctgggg
    10621 aaataaacca tggcaaagac tattaaaatt actcaaaccc gcagtgcaat cggtcgtctg
    10681 ccgaaacaca aggcaacgct gcttggcctg ggtctgcgtc gtattggtca caccgtagag
    10741 cgcgaggata ctcctgctat tcgcggtatg atcaacgcgg tttccttcat ggttaaagtt
    10801 gaggagtaag agatgcgttt aaatactctg tctccggccg aaggctccaa aaaggcgggt
    10861 aaacgcctgg gtcgtggtat cggttctggc ctcggtaaaa ccggtggtcg tggtcacaaa
    10921 ggtcagaaat ctcgttctgg cggtggcgta cgtcgcggtt tcgagggtgg tcagatgcct
    10981 ctgtaccgtc gtctgccgaa attcggcttc acttctcgta aagcagcgat tacagccgaa
    11041 gttcgtctgt ctgacctggc taaagtagaa ggcggtgttg tagacctgaa cacgctgaaa
    11101 gcggctaaca ttatcggtat ccagatcgag ttcgcgaaag tgatcctggc tggcgaagta
    11161 acgactccgg taactgttcg tggcctgcgt gttactaaag gcgctcgtgc tgctatcgaa
    11221 gctgctggcg gtaaaatcga ggaataagta gcagatggct aaacaaccgg gattagattt
    11281 tcaaagtgcc aaaggtggct taggcgagct gaaacgcaga ctgctgtttg ttatcggtgc
    11341 gctgattgtg ttccgtattg gctcttttat tccgatccct ggtattgatg ccgctgtact
    11401 tgccaaactg cttgagcaac agcgaggcac catcattgag atgtttaaca tgttctctgg
    11461 tggtgctctc agccgtgctt ctatctttgc tctggggatc atgccgtata tttcggcgtc
    11521 gatcattatc cagctgctga cggtggttca cccaacgttg gcagaaatta agaaagaagg
    11581 ggagtctggt cgtcgtaaga tcagccagta cacccgttac ggtactctgg tgctggcaat
    11641 attccagtcg atcggtattg ctaccggtct gccgaatatg cctggtatgc aaggcctggt
    11701 gattaaccca ggctttgcat tctacttcac cgctgttgta agtctggtca caggaacgat
    11761 gttcctgatg tggttgggcg aacagattac tgaacgaggt atcggcaacg gtatttcaat
    11821 cattatcttc gccggtattg tcgcgggact cccgccagcc attgcccata ctatcgagca
    11881 agcgcgtcaa ggcgacctgc acttcctcgt gttgctgttg gttgcagtat tagtatttgc
    11941 agtgacgttc tttgttgtat ttgttgagcg tggtcaacgc cgcattgtgg taaactacgc
    12001 gaaacgtcag caaggtcgtc gtgtctatgc tgcacagagc acacatttac cgctgaaagt
    12061 gaatatggcg ggggtaatcc cggcaatctt cgcttccagt attattctgt tcccggcgac
    12121 catcgcgtca tggttcgggg gcggtactgg ttggaactgg ctgacaacaa tttcgctgta
    12181 tttgcagcct gggcaaccgc tttatgtgtt actctatgcg tctgcaatca tcttcttctg
    12241 tttcttctac acggcgttgg ttttcaaccc gcgtgaaaca gcagataacc tgaagaagtc
    12301 cggtgcattt gtaccaggaa ttcgtccggg agagcaaacg gcgaagtata tcgataaagt
    12361 aatgacccgc ctgaccctgg ttggtgcgct gtatattacc tttatctgcc tgatcccgga
    12421 gttcatgcgt gatgcaatga aagtaccgtt ctacttcggt gggacctcac tgcttatcgt
    12481 tgttgtcgtg attatggact ttatggctca agtgcaaact ctgatgatgt ccagtcagta
    12541 tgagtctgca ttgaagaagg cgaacctgaa aggctacggc cgataattgg tcgcccgaga
    12601 agttacggag agtaaaaatg aaagttcgtg cttccgtcaa gaaattatgc cgtaactgca
    12661 aaatcgttaa gcgtgatggt gtcatccgtg tgatttgcag tgccgagccg aagcataaac
    12721 agcgccaagg ctgatttttt cgcatatttt tcttgcaaag ttgggttgag ctggctagat
    12781 tagccagcca atcttttgta tgtctgtgcg tttccatttg agtatcctga aaacgggctt
    12841 ttcagcatgg aacgtacata ttaaatagta ggagtgcata gtggcccgta tagcaggcat
    12901 taacattcct gatcataagc atgccgtaat cgcattaact tcgatttatg gcgtcggcaa
    12961 gacccgttct aaagccatcc tggctgcagc gggtatcgct gaagatgtta agatcagtga
    13021 gctgtctgaa ggacaaatcg acacgctgcg tgacgaagtt gccaaatttg tcgttgaagg
    13081 tgatctgcgc cgtgaaatca gcatgagcat caagcgcctg atggatcttg gttgctatcg
    13141 cggtttgcgt catcgtcgtg gtctcccggt tcgcggtcag cgtaccaaga ccaacgcacg
    13201 tacccgtaag ggtccgcgca aaccgatcaa gaaataatcg gggtgattga ataatggcaa
    13261 aggcaccaat tcgtgcacgt aaacgtgtaa gaaaacaagt ctctgacggc gtggctcata
    13321 tccatgcttc tttcaacaac accatcgtga ctatcactga tcgtcagggt aacgcgttgg
    13381 gttgggcaac agccggtggt tccggtttcc gtggttctcg caaatccact ccgtttgcag
    13441 ctcaggttgc agcagagcgt tgcgctgacg ccgtgaaaga atacggcatc aagaatctgg
    13501 aagttatggt taaaggtccg ggtccaggcc gcgaatctac tattcgtgct ctgaacgccg
    13561 caggtttccg catcactaac attactgatg tgactccgat ccctcataac ggttgtcgtc
    13621 cgccgaaaaa acgtcgcgta taacgcctcg ttttccaggt ttgttggaga aagaaaatgg
    13681 caagatattt gggtcctaag ctcaagctga gccgtcgtga gggcaccgac ttattcctta
    13741 agtctggcgt tcgcgcgatc gataccaagt gtaaaattga acaagctcct ggccagcacg
    13801 gtgcgcgtaa accgcgtctg tctgactatg gtgtgcagtt gcgtgaaaag caaaaagttc
    13861 gccgtatcta tggtgtgctg gagcgtcagt tccgtaacta ctacaaagaa gcagcacgtc
    13921 tgaaaggcaa caccggtgaa aacctgttgg ctctgctgga aggtcgtctg gacaacgttg
    13981 tataccgtat gggcttcggt gccactcgtg cagaagcacg tcagctggtt agccataaag
    14041 caattatggt aaacggtcgt gttgttaaca tcgcttctta tcaggttagt ccgaatgacg
    14101 ttgtaagcat tcgtgagaaa gcgaagaagc agtctcgcgt gaaagccgct ctggagctgg
    14161 ctgagcagcg tgaaaagcca acctggctgg aagttgatgc tggcaagatg gaaggtacgt
    14221 ttaagcgtaa gccggagcgt tctgatctgt ctgcggacat taacgaacac ctgatcgtcg
    14281 agctttactc caagtaaagc ttagtaccaa agagaggaca caatgcaggg ttctgtgaca
    14341 gagtttctaa aaccgcgcct ggttgatatc gagcaagtga gttcgacgca cgccaaggtg
    14401 acccttgagc ctttagagcg tggctttggc catactctgg gtaacgcact gcgccgtatt
    14461 ctgctctcat cgatgccggg ttgcgcggtg accgaggttg agattgatgg tgtactacat
    14521 gagtacagca ccaaagaagg cgttcaggaa gatatcctgg aaatcctgct caacctgaaa
    14581 gggctggcgg tgagagttca gggcaaagat gaagttattc ttaccttgaa taaatctggc
    14641 attggccctg tgactgcagc cgatatcacc cacgacggtg atgtcgaaat cgtcaagccg
    14701 cagcacgtga tctgccacct gaccgatgag aacgcgtcta ttagcatgcg tatcaaagtt
    14761 cagcgcggtc gtggttatgt gccggcttct acccgaattc attcggaaga agatgagcgc
    14821 ccaatcggcc gtctgctggt cgacgcatgc tacagccctg tggagcgtat tgcctacaat
    14881 gttgaagcag cgcgtgtaga acagcgtacc gacctggaca agctggtcat cgaaatggaa
    14941 accaacggca caatcgatcc tgaagaggcg attcgtcgtg cggcaaccat tctggctgaa
    15001 caactggaag ctttcgttga cttacgtgat gtacgtcagc ctgaagtgaa agaagagaaa
    15061 ccagagttcg atccgatcct gctgcgccct gttgacgatc tggaattgac tgtccgctct
    15121 gctaactgcc ttaaagcaga agctatccac tatatcggtg atctggtaca gcgtaccgag
    15181 gttgagctcc ttaaaacgcc taaccttggt aaaaaatctc ttactgagat taaagacgtg
    15241 ctggcttccc gtggactgtc tctgggcatg cgcctggaaa actggccacc ggcaagcatc
    15301 gctgacgagt aaccggatca caggttaagg ttttactgag aaggataagg tcatgcgcca
    15361 tcgtaagagt ggtcgtcaac tgaaccgcaa cagcagccat cgccaggcta tgttccgcaa
    15421 tatggcaggt tcactggttc gtcatgaaat catcaagacg actctgccta aagcgaaaga
    15481 gctgcgccgc gtagttgagc cgctgattac tcttgccaag actgatagcg ttgctaatcg
    15541 tcgtctggca ttcgcccgta ctcgtgataa cgagatcgtg gcaaaactgt ttaacgaact
    15601 gggcccgcgt ttcgcgagcc gtgccggtgg ttacactcgt attctgaagt gtggcttccg
    15661 tgcaggcgac aacgcgccga tggcttacat cgagctggtt gatcgttcag agaaagcaga
    15721 agctgctgca gagtaatctg aagcaacgta aaaaaacccg ccccggcggg tttttttata
    15781 cccgtagcat ccccacttat ctacaatagc tgtactcttt ttgttcatcc cctggagtat
    15841 ttatgtggtt acttgaccag tgggcagagc gtcatatagc agaagcgcaa gcgaaaggtg
    15901 agtttgataa cctggcaggt agcggcgaac cattgatact ggatgatgat tctcacgtgc
    15961 caccggaatt acgtgcgggg tatcgcttgc tgaagaatgc cggttgctta ccgccagaac
    16021 ttgagcaacg gagagaagca attcagcttc tggatatcct gagaggtatc cgtcacgatg
    16081 atccgcaata tcaagaggtt agccgtcgat tgtcattact ggaattgaag ctgcgacaag
    16141 ctggattgag taccgatttt ttacgcggcg attatgctga caagttgttg gacaaaatca
    16201 acgataacta gtggagtatg tatgtatcgc attggtgagc tggcaaaaat ggcggaagta
    16261 acacctgaca cgattcgtta ttacgaaaaa cagcagatga tggagcatga agtgcgtact
    16321 gaaggcgggt ttcgcctgta taccgaaagc gatctccagc gattgaaatt tatccgccat
    16381 gccagacaac taggtttcag tctggagtcg atccgcgagt tgctgtcgat ccgcatcgat
    16441 cctgaacacc atacctgtca ggagtcaaaa ggcattgtgc aggaaagatt gcaggaagtc
    16501 gaagcacgga tagccgagtt gcagagtatg cagcgttcct tgcaacgcct taacgatgcc
    16561 tgttgtggga ccgcccatag cagtgtttat tgttcgattc ttgaagctct tgaacaaggg
    16621 gcgagtggcg ttaagagtgg ctgttgattt tttgcactgg caggattaca ctcgcgccgt
    16681 aaaataacca attggagttt ttatgagccg atatcagcat actaaagggc agataaagga
    16741 taatgcgata gaagcattac tacatgatcc cttattccga cagcgcgtag agaaaaataa
    16801 gaaggggaaa ggcagttaca tgcgaaaagg aaaacatggc aatcggggga actgggaggc
    16861 cagtggcaag aaagtaaatc acttttttac cactggcctt ctgctttcag gcgtttgtta
    16921 agagcggtta ttctgctctt tcagcaaatc acgaatttct gtcagtaata cttcttcttt
    16981 agttggtgct ggtgcggctg ctggttcttc ttttttccga ttcagtttgt tgattagctt
    17041 aatcgccata aagatggcaa aggccacaat cagaaaatca aagacgtttt gaatgaagac
    17101 accgtaatgc atcacaacag cagggatatc cccctgcgca tcgcgtagcg tgacagcaaa
    17161 ctgtttaaag tcgatcccgc caattaataa gcccagaggt ggcatgatga tatcggcaac
    17221 cagtgaagat acaatcttcc cgaatgccgc accgataatg acacccaccg ccaaatccac
    17281 cacgttcccg cgcatcgcaa attcgcgaaa ttctttaata atgctcatgt tattctccct
    17341 atgaagccga caaccataag tctaacaaat gttaagccat tttcctgcca ggaatgaatt
    17401 taattaatcc ttgataatca aagggaaata ataaggcgtc attagacgcc ttattaatta
    17461 caagaagaaa gggcttggct ggaagagtct ttcgacgtcg gtaataaact ttttatctgt
    17521 gaggaacata attacgtgat cgccttgctc aatgcgcaga ttgtcattgg caatcatcac
    17581 gtcgtttcca cgtaccaccg ctccaataat cgttcctggc ggtagcttga tttcgtcaat
    17641 gactctgccg acaacgcgtg aggtgctttc atcaccgtga gcaacggctt caatagcttc
    17701 tgctacgccg cggcgcaatg aggaaacacc aacaatatct gcttttcgca catggctaag
    17761 caacgcagaa atagttgctt gttgtggtga aatcgcaata tcgatcacgc tcccctgaac
    17821 cagatccaca taagcgcgac gctggatcaa taccatcacc tttttcgcac ccatacgttt
    17881 ggcaagcatg gcggacatga tattggcctc gtcatcgttg gtgacggcaa taaacagatc
    17941 aacttgatcg atatgttctt cggccagcag ttcttgatcc gacgcatcac caaaaaagac
    18001 aatcgtattc tgcaactttt ccgccagttc ggcagcgcgc tgctgattac gttcgatgag
    18061 tttaacgctg taatcttttt ccagacgacg cgccagccct gcaccgatat taccgccacc
    18121 aaccagcatg atccgcttat acggtttttc cagtcgctgt aattcactca tcaccgcacg
    18181 gatatgctgt gaagcggcaa taaagaacac ttcatcacca gcttccacaa tggtcgaacc
    18241 ttgtggacga atggggcgat cgtggcggaa aattgctgcc acacgagtat cgatatgtgg
    18301 catatgttcg cgcatggtcg aaagtgcgtt accaatcagc gggccgccat aataggcttt
    18361 aaccacagcg aggctgactt taccctcagc gaagttcacc acctgcaatg cgccgggata
    18421 ctcaatcagt cggtaaatat tatcgataac caactgctct ggtgcgatca gatgatcaat
    18481 cggtacagca tctgaatgaa atagcttatc ggcatcgcgc acgtagtctg gtgagcggat
    18541 gcgagcgatg cgattagggg tgttgaaaag tgagtaggct acctggcagg caaccatatt
    18601 ggtttcatct gaactggtta cagcaaccag catatcggcg tcgtcggcac ctgcctcccg
    18661 caatacgcgt ggatgagagc catgcccctg cacgactcgc aggtcaaatt tatcctgcaa
    18721 ggtccgcaga cgctcaccgt tggtatcgac aacagtgata tcgttgttct cgccaaccag
    18781 gttttccgcc agtgtgccgc caacctggcc ggcacccaga atgataattt tcatcagtcg
    18841 cgacccgtta tctcatcact ttttgattag cttagcgtaa aagaagccgt cgccctcttc
    18901 ggtaccaggc agattttgtt tacccggttg ctctggtgtt cctgtttcgc aaagttcggc
    18961 atcagcggta cgttgcaaaa aggctttaat ctgcaggcta ttctcttccg gtaacaccga
    19021 acaggtggca tagaccaggg ttccaccgga ttttaaatgc ggccaaatgg cgtcgagaat
    19081 ttcagactgc aattgcgcga gttccgggat atcgcgatcg cgacgtaacc atttaatatc
    19141 cggatggcga cgaatcacac cggttgctga acaaggcgca tctaataaaa tgcgatcaaa
    19201 ctgttgctcg ccacaccatt gggaagggta gcggccatca ccttgtttca cggtcgcttt
    19261 cataccaagg cgttttaaat tgtcgtaaac gcgagagagg cgctgttcgt caatatcaac
    19321 cgcaacaacc tgcgcttctg gtgccacctc aaggatatgc gttgttttac cgccgggggc
    19381 ggcacaaaga tccagaatgt gttcaccgtt ttgtggcgca agccaggtca tgcaaccttg
    19441 tgctgatgca tcctgaacgg taacccatcc gtcttcaaaa ccaggtagcg catgaacagg
    19501 tgcaggtgtt tccagccgta cagcatcagg gtaatccgca tgcgggaaac ctttcattcc
    19561 tgcttcatcc aacaatgcaa gccagctgtc gcgggaatga tgcgtacgat taacgcgcag
    19621 ccacattggc ggacgctggt tattggcttc gacgatggat tgccactgct ctggatacgc
    19681 tttttgcaga cgcttcagca accaggaagg atgcagataa cgtgcatcac tggcattaaa
    19741 ctcggctaat aactcttctt gctgacgctg gaactggcgt aatacaccgt ttatcaaccc
    19801 tttaagttgc ggacgcttaa ttgcgatagc gccttcaacc gtttcagcca gcgcagcatg
    19861 aggtggaatg cgggtataaa gcagttgata caaaccaacc ataatcaggt aatgcacggt
    19921 ccgctgtttg ccggtcatcg gacgggccat taacttatta atcagccagt ctaactgcga
    19981 aagcgtacgc agtacgccaa agcacaactc ttgaagaagt gctttgtctt tatcggaaac
    20041 tttttgctgg agcggtggca gaatgttgct taatgattgc ccttgctcga cgacttgttc
    20101 aacggcctgg gccgccatgc tacgtaaatt acgttgtttt ttcataagta taaaaatgcc
    20161 cggcaagacc gggcttagaa gagtggacta tcagaccaga cggttgcccg gaacaaacca
    20221 ttcccgacga gagttcagga ggtcttgcgc gctcatcgct ttcttacccg caggttgtaa
    20281 cgagagcagg ttcaggatgc catcaccagt cgcaacctga atgccttgtt tattggcttc
    20341 aaggatcgtt cctggtgcag cgttggttgc cgtatcaatg accgacgctt tccagacttt
    20401 aaccggttgt ccttcaattt ccagccagct cattggccat gggttgaaag cgcgaatgca
    20461 gcgttcaagc tgcgctgccg aaagcgacca gtcaatacgc gcttcttctt tactcaactt
    20521 ctcggcgtaa gtgacaagag tttcgtcctg aacttctggt ttcgccgtgc cgtctgccag
    20581 ttgtttcaac gtggtgataa gcccttgtgg gccaagctct gccagcttgt cgtacagcgt
    20641 accactggta tcttctgcag taatcgggca ggagagctta tagagcatat caccggtgtc
    20701 taaaccgaca tccatttgca taatggtcat accagtttct gcatcacccg cccatagtga
    20761 gcgttggatt ggtgcagcac cgcgccagcg tggcagcagt gaaccatgaa cgttgataca
    20821 gccaagacgc ggcatctcca gcactgcttt aggcagaatt aagccatagg cgacgacgac
    20881 cataacatca gcctgcagat cggcgaccag ttgctggttt tcttgtggac gcagggaaac
    20941 aggttgaaaa acgggcagac ctttttcctc agccagaact ttaaccgggc tgggcatcag
    21001 ttttttaccg cgtcctgccg gtcggtctgg ctgggtgaac acgccaacga cgttatgacc
    21061 agaagacaac agcgcgtcga gatgacgcgc tgcaaagtca ggtgtacccg caaaaataat
    21121 acgtagtgat tctgacacgt tagttcttat ccttaagccc gggctttcag acgatccagt
    21181 ttttcaactt tctgacgaat acgttgttgt ttcaacggtg acagataatc cataaacagt
    21241 ttgccgacca ggtgatccat ctcatgctga atacagatgg ctaacagacc gtctgcttcc
    21301 agttcaaatg gtttaccgtc gcggtccagg gcgcgaattt taactttctc tgcgcgtggc
    21361 actaaagcac gttgttcagg gatcgacagg caaccttctt caatgcctgt ttcgccgctt
    21421 ttttctaaaa gctccggatt gattaacacc agccgttcgt cacggttttc cgaaacatca
    21481 ataacaatga tacgttgatg gatatcaacc tgggttgccg ccaggccaat accttcttct
    21541 gcgtacatcg tctcgaacat atcatcgacg atacgctgaa tttctgcatt cacttcttct
    21601 accggtttag caactttgcg aagccgctcg tccggaatat gtaacacttg caaaactgac
    21661 ataaatctcc agagatgtgt tcaggagtta gaaagattat ttcttctatt ctagacaaat
    21721 ccccctctga ttgacagcat cactgaccaa tcgcaaagat tgctaaggct gcttatggca
    21781 gggagataag gatggtcgat acagatattt ggctgcgttt aatgagcatc agcagcttgt
    21841 acggcgatga tatggtccgt atagctcact ggctggcaaa acagtcgcat attgatgcgg
    21901 ttgtattgca gcaaacaggg cttacattgc ggcaggcaca acgctttctt tcatttccgc
    21961 gaaagagtat cgaaagctca ctttgttggt tggagcaacc caaccatcat ttaatccctg
    22021 cggacagcga attttatcct cctcaacttc tggtgacgac agattacccc ggcgcactgt
    22081 ttgttgaagg agaactgcac gcgctgcatt catttcagct tgccgtagtg gggagtcggg
    22141 cgcattcatg gtatggcgag cgatggggac ggttattttg cgaaactctg gcgacgcgtg
    22201 gagtgacaat tacgagtgga ctggcgcgtg gaatcgatgg tgtggcgcat aaagctgcct
    22261 tacaggtaaa tggcgtcagc attgctgtat tggggaatgg acttaatacc attcatcccc
    22321 gccgccatgc ccgactggct gccagtctgc ttgaacatgg cggagctctc gtctcggaat
    22381 ttcccctcga tgttccaccc cttgcttaca atttcccacg aagaaatcgc attatcagtg
    22441 gtctaagtaa aggtgtactg gtggtggaag cggctttgcg cagtggttcg ctggtgacag
    22501 cacgttgtgc gcttgagcag gggcgtgaag tttttgcctt gccaggtcca atagggaatc
    22561 cgggaagcga agggcctcac tggttaataa aacaaggtgc gattcttgtg acggaaccgg
    22621 aagaaattct ggaaaacttg caatttggat tgcactggtt gccagacgcc cctgaaaatt
    22681 cattttattc accagatcag caagacgtgg cattgccatt tcctgagctc ctggctaacg
    22741 taggagatga ggtaacacct gttgacgtcg tcgctgaacg tgccggccaa cctgtgccag
    22801 aggtagttac tcaactactc gaactggagt tagcaggatg gatcgcagct gtacccggcg
    22861 gctatgtccg attgaggagg gcatgccatg ttcgacgtac taatgtattt gtttgaaacc
    22921 tatattcaca cagaagctga gttgcgtgtg gatcaagaca aacttgaaca ggatcttacc
    22981 gacgcaggat ttgatcgaga agatatctac aatgccctgc tttggctgga aaagcttgcg
    23041 gattatcagg aaggactggc agaaccgatg caactggcct ctgatccact ctccatgcgt
    23101 atttataccc ctgaggagtg tgaaagactg gatgccagct gtcgtggttt tctgcttttc
    23161 cttgagcaaa ttcaggtgct taaccttgaa acccgtgaaa tggtgataga gcgagtgctc
    23221 gcgctggata ccgcagagtt cgacctggaa gacctgaaat gggtaatcct gatggtgttg
    23281 ttcaatattc cgggctgcga aaatgcgtac cagcaaatgg aagaattact ctttgaagtg
    23341 aatgaaggta tgctgcatta atcatttctt taattcagca taagttgtta tggcgaaatc
    23401 agcactgttc acggtgcgta ataatgagtc ctgcccaaag tgcggggctg aactggttat
    23461 tcgatccggg aaacacggtc cgtttcttgg atgctcacag tatccggcgt gtgactacgt
    23521 ccgtcctctg aaatcttcag cggatggaca tattgtcaaa gttctggagg ggcaggtttg
    23581 ccttgcatgt ggcgcaaatc tggtattacg ccagggacgc tttggtatgt ttattggttg
    23641 cagtaactac cctgaatgcg aacataccga acttatcgat aaaccggacg aaacagcaat
    23701 tacatgcccc caatgccgga cgggccatct ggtccagcgc cgctcccgtt atggcaaaac
    23761 atttcactcc tgtgatcgct acccggagtg tcaatttgcc attaacttca aacccatagc
    23821 tggagaatgc cctgagtgtc attatccact actcatcgaa aagaaaaccg cgcagggtgt
    23881 aaaacacttt tgtgccagta aacaatgtgg aaagccggtt tcggcggaat aataacgtga
    23941 ataataacct gcaaggagac gctatcgcag ctgcgataga tgtcctcaat gaagaacgtg
    24001 tcatcgccta tccaacggaa gccgttttcg gtgttgggtg cgatcctgat agcgaaacag
    24061 cagtgatgag actgttggag ttaaaacagc gtccggttga taaggggctg attttaatcg
    24121 cggcaaatta cgagcagctt aaaccctata ttgatgacac catgctgact gatgcgcagc
    24181 gtgaaaccat tttttcccgc tggccaggtc ctgtcacctt tgtctttccc gcgccagcga
    24241 caacaccgcg ctggttgacg ggccgctttg attcgcttgc tgtacgagtc accgaccatc
    24301 cgttggtggt tgctttgtgc caggcttatg gtaaaccgct ggtttctacc agtgccaact
    24361 tgagtggatt gccaccttgt cgaacagtag acgaagttcg cgcacaattt ggcgcggcgt
    24421 tcccggttgt gcctggtgaa acgggggggc gtttaaatcc ttcagaaatc cgcgatgccc
    24481 tgacgggtga actgtttcga caggggtaac ataatggaaa cctatgctgt ttttggtaat
    24541 ccgatagccc acagcaaatc gccattcatt catcagcaat ttgctcaaca actgaatatt
    24601 gaacatccct atgggcgcgt gttggcaccc atcaatgatt tcatcaacac actgaacgct
    24661 ttctttagtg ctggtggtaa aggtgcgaat gtgacggtac cttttaaaga agaggctttt
    24721 gccagagcgg atgagcttac tgaacgggca gcgttggctg gtgctgttaa taccctcaag
    24781 cggttagaag atggacgctt gcagggtgac aataccgatg gtataggttt gttaagcgat
    24841 ctggaacgtc tgtcttttat ccgccctggt ttacgtattc tgcttatcgg cgctggtgga
    24901 gcatctcgcg gcgtactact gccacttctt tccctggact gtgcggtgac aatcactaat
    24961 cggacggtat cccgcgcgga agagttggct aaattgtttg cgcacactgg tagtattcag
    25021 gcgttgagta tggacgaact ggaaggacat gagtttgatc tcattattaa tgcaacatcc
    25081 agtggcatca gtggtgatat tccggcgatc ccgtcatcgc tcattcatcc aggcatttat
    25141 tgctatgaca tgttctatca gaaaggaaaa actccttttc tggcatggtg tgagcagcga
    25201 ggctcaaagt gtaatgctga tggtttagga atgctggtgg cacaggcggc tcatgccttt
    25261 cttctctggc acggtgttct gcctgacgta gaaccagtta taaagcaatt gcaggaggaa
    25321 ttgtccgcgt gaatcaggcc atccagtttc cggacagaga agagtgggac gagaataaaa
    25381 aatgtgtatg ttttcccgct ctcgtgaatg gcatgcaact gacatgtgcg atctctggcg
    25441 agagtctggc gtatcgtttt actggagata cgccagaaca gtggttagcg agttttcgtc
    25501 agcatcgctg ggatctggaa gaagaagcgg aaaacttaat tcaggaacaa agtgaagatg
    25561 atcaaggctg ggtctggtta ccctgatcca gatattcgtc cttccatttc acgtaattat
    25621 tcgcggaata gcgtaaccca gccttctctt catcacttaa cgggcggatc tgtttgacgg
    25681 ggctaccgag atacagatat ccgctctcca gccgtttatt ttgtgggacc agactacccg
    25741 caccaatcat cacatcatct tctactattg cgccatcaag taaaattgag cccatcccaa
    25801 ccaaaactcg attgccaatg gtgcagccgt ggagcatcac cttgtgacca acagtgacat
    25861 cttcgccaat ggttaatggg ttgccatctg ggttgtacga ggatttatga gtgacatgca
    25921 acatactgcc atcctggata ttggtgcgtg ctccgatctg tacataatgt acatctccac
    25981 gaatcacaac gagcggccag atccccacat catcagccag acgaacgtca ccaatcacga
    26041 cactgctatc gtcgatcatt acgcgctgac cgatttgtgg aaaaagatcg cggtatgggc
    26101 gtaaaacatc agacatactt acctcagcaa taaatgattt actaatgact ttgggggcat
    26161 tattggcctt gtgcaagtct tttagtatgc aaaaaagcac cgttttgtgt gcgattgcag
    26221 caaaaagggt gaaaaaacaa caaacagaaa aaaagatcaa aaaaatactt gtgcaaaaaa
    26281 ttgggatccc tataatgcgc ctccgttgag acgacaacgt gaaacacttc acaagatggt
    26341 cgtaacaacg aagagaaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta
    26401 atatacgcca cctcgcgaca gtgagctgaa agccgcgtcg caactgctct ttaacaattt
    26461 atcagacaat ctgtgtgggc actcgaagat acggattctt aacgtcgcaa gacgaaaaat
    26521 gaataccaag tctcaagagt gaacacgtaa ttcattacga agtttaattc tttgagcatc
    26581 aaacttttaa attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac
    26641 acatgcaagt cgaacggtaa caggaaacag c
//
LOCUS       DAFNBJ010000059        26499 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000059 DAFNBJ010000000
VERSION     DAFNBJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.059"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            97..1206
                     /gene="anmK"
                     /locus_tag="L2X49_002312"
     CDS             97..1206
                     /gene="anmK"
                     /locus_tag="L2X49_002312"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBN4797392.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVVPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYLNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCKRLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            1304..1633
                     /gene="mliC"
                     /locus_tag="L2X49_002313"
     CDS             1304..1633
                     /gene="mliC"
                     /locus_tag="L2X49_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBN4797393.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            1692..2348
                     /gene="pdxH"
                     /locus_tag="L2X49_002314"
     CDS             1692..2348
                     /gene="pdxH"
                     /locus_tag="L2X49_002314"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBN4797394.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            2477..3751
                     /gene="tyrS"
                     /locus_tag="L2X49_002315"
     CDS             2477..3751
                     /gene="tyrS"
                     /locus_tag="L2X49_002315"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBN4797395.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            3810..4673
                     /gene="pdxY"
                     /locus_tag="L2X49_002316"
     CDS             3810..4673
                     /gene="pdxY"
                     /locus_tag="L2X49_002316"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBN4797396.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(4717..5322)
                     /gene="gstA"
                     /locus_tag="L2X49_002317"
     CDS             complement(4717..5322)
                     /gene="gstA"
                     /locus_tag="L2X49_002317"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBN4797397.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGFEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(5428..6930)
                     /gene="dtpA"
                     /locus_tag="L2X49_002318"
     CDS             complement(5428..6930)
                     /gene="dtpA"
                     /locus_tag="L2X49_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBN4797398.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(7541..8176)
                     /gene="nth"
                     /locus_tag="L2X49_002319"
     CDS             complement(7541..8176)
                     /gene="nth"
                     /locus_tag="L2X49_002319"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBN4797399.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(8176..8871)
                     /gene="rsxE"
                     /locus_tag="L2X49_002320"
     CDS             complement(8176..8871)
                     /gene="rsxE"
                     /locus_tag="L2X49_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBN4797400.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(8875..9495)
                     /gene="rsxG"
                     /locus_tag="L2X49_002321"
     CDS             complement(8875..9495)
                     /gene="rsxG"
                     /locus_tag="L2X49_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBN4797401.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(9499..10557)
                     /gene="rsxD"
                     /locus_tag="L2X49_002322"
     CDS             complement(9499..10557)
                     /gene="rsxD"
                     /locus_tag="L2X49_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBN4797402.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(10558..12684)
                     /gene="rsxC"
                     /locus_tag="L2X49_002323"
     CDS             complement(10558..12684)
                     /gene="rsxC"
                     /locus_tag="L2X49_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBN4797403.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQSNAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDP
                     RKAAVAAAIARAQAKKAAQQKVVNED"
     gene            complement(12677..13255)
                     /gene="rsxB"
                     /locus_tag="L2X49_002324"
     CDS             complement(12677..13255)
                     /gene="rsxB"
                     /locus_tag="L2X49_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBN4797404.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(13255..13836)
                     /gene="rsxA"
                     /locus_tag="L2X49_002325"
     CDS             complement(13255..13836)
                     /gene="rsxA"
                     /locus_tag="L2X49_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBN4797405.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(13913..14353)
                     /locus_tag="L2X49_002326"
     CDS             complement(13913..14353)
                     /locus_tag="L2X49_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBN4797406.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(14439..14654)
                     /gene="ydgT"
                     /locus_tag="L2X49_002327"
     CDS             complement(14439..14654)
                     /gene="ydgT"
                     /locus_tag="L2X49_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBN4797407.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(14927..15052)
                     /gene="blr"
                     /locus_tag="L2X49_002328"
     CDS             complement(14927..15052)
                     /gene="blr"
                     /locus_tag="L2X49_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBN4797408.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            15295..16335
                     /locus_tag="L2X49_002329"
     CDS             15295..16335
                     /locus_tag="L2X49_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBN4797409.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(16370..17371)
                     /gene="add"
                     /locus_tag="L2X49_002330"
     CDS             complement(16370..17371)
                     /gene="add"
                     /locus_tag="L2X49_002330"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBN4797410.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLAKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQTQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(17475..18647)
                     /gene="malY"
                     /locus_tag="L2X49_002331"
     CDS             complement(17475..18647)
                     /gene="malY"
                     /locus_tag="L2X49_002331"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBN4797411.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNATFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(18657..20249)
                     /gene="malX"
                     /locus_tag="L2X49_002332"
     CDS             complement(18657..20249)
                     /gene="malX"
                     /locus_tag="L2X49_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBN4797412.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLNC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            20424..21452
                     /gene="malI"
                     /locus_tag="L2X49_002333"
     CDS             20424..21452
                     /gene="malI"
                     /locus_tag="L2X49_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBN4797413.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            21564..22331
                     /gene="hdhA"
                     /locus_tag="L2X49_002334"
     CDS             21564..22331
                     /gene="hdhA"
                     /locus_tag="L2X49_002334"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBN4797414.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            22560..23150
                     /gene="uidR"
                     /locus_tag="L2X49_002335"
     CDS             22560..23150
                     /gene="uidR"
                     /locus_tag="L2X49_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBN4797415.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            23539..25350
                     /gene="uidA"
                     /locus_tag="L2X49_002336"
     CDS             23539..25350
                     /gene="uidA"
                     /locus_tag="L2X49_002336"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBN4797416.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAANKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            25347..>26499
                     /gene="uidB"
                     /locus_tag="L2X49_002337"
     CDS             25347..>26499
                     /gene="uidB"
                     /locus_tag="L2X49_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBN4797417.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAF"
BASE COUNT         6647 a         6636 c         6880 g         6336 t
ORIGIN      
        1 ttactaccaa cggtaaaaat cggtcattcc gcatggtatt cacgaaaagt ttatgtttga
       61 gtggtgcgca aacgtcagat tgaaaaagga tgaactatga aatcgggccg ctttattggc
      121 gttatgtcag gcaccagcct tgatggtgtt gatgttgtgt tggcgacaat agatgaacac
      181 cgggtcgcac agctggcaag cttgagctgg ccgatcccgg tgtctctgaa acaggctgta
      241 ctggatattt gccagggcca gcagcttaca ctttcgcagt ttggacagct tgatactcaa
      301 ctcgggcgac tttttgctga tgcggtcaat gccttgctta aggaacaaaa cctgcaggcg
      361 agagatatag ttgcgatcgg ctgtcacggt caaaccgtct ggcatgaacc gacgggcgtg
      421 gtaccacaca ctttacagat tggcgataac aatcaaattg tggcacgcac cggaattacg
      481 gttgtcggtg attttcgccg tcgcgatatt gccttgggag gacaaggcgc accgctggta
      541 cctgcgttcc atcatgcctt gctggctcac ccaaccgagc gacgaatggt gctcaatatt
      601 ggcggcatcg ccaatctgtc actgctcatt cctgggcagc cggttggcgg ctacgatacc
      661 ggtcctggta acatgctgat ggatgcctgg atctggcgtc aggccggtaa accttacgat
      721 aaagatgccg agtgggcacg ggcgggtaaa gttattctcc cactgctgca aaatatgctc
      781 agcgacccat atttctcgca acctgcaccg aaaagcaccg gacgcgaata cttaaactac
      841 ggctggctgg agcgccattt acgccatttt ccgggtgttg atccccgtga tgttcaggcc
      901 acactggcag aactcaccgc cgtgaccatt tctgaacaag ttttgttgag cggtggctgc
      961 aaacgattga tggtatgtgg tggaggtagt cgtaatccgc tactcatggc gcgtctggcg
     1021 gcattactgc caggcacaga agtcaccacc accgatgccg ttggcattag tggcgatgac
     1081 atggaagcat tggctttcgc ctggcttgcc tggcggacgc tggcgggatt accaggaaat
     1141 ctgccttccg tcactggcgc aagccaggag acggtactgg gggctatttt ccccgctaac
     1201 ccgtgatatc agagttaact gaatttttct taccgcgtct gccgttacac ttggaacgtt
     1261 agggagggcg tattgccctc cagaccagga aagtcttcgg gatatgacta tgaaaaaact
     1321 gttaattatc attttgccgg tccttctctc cggctgtagc gcctttaatc agctggttga
     1381 gcgcatgcaa accgatacgc tggaatacca gtgtgatgaa aaaccgttga cggtcaaact
     1441 gaataatccg cgccaggagg tcagttttgt ttacgataat caactactgc atctcaaaca
     1501 gggcatttca gcctctggcg cgcgttacac tgacggaatc tatgttttct ggtcgaaagg
     1561 cgatgaagcg actgtctata aacgcgaccg catcgtcttg aataactgtc agttacaaaa
     1621 tccacagcgt tgagattttt ccaggggcgg cgcacaatag cgtcacccac tgacaatccg
     1681 taaagaaaac catgtctgat aacgacgaat tgcagcaaat cgcgcatctg cgccgtgaat
     1741 acaccaaagg cgggttacgc cgccgcgatc ttcccgccga tccattaacc ctttttgaac
     1801 gttggctctc tcaggcttgt gaagccaaac tggcggaccc taccgcgatg gtggtcgcta
     1861 ccgtggatga acatggtcag ccttatcagc gcatcgtttt actcaaacat tacgacgaaa
     1921 aaggcatggt gttttacacc aacctcggca gccgtaaagc acatcaaatc gaaaataatc
     1981 cgcgcgttag cctgctgttc ccgtggcata cccttgagcg ccaggtgatg gtgatcggta
     2041 aagcagaacg actttcgact ctcgaagtga tgaaatattt tcatagccgc ccgcgtgata
     2101 gccagattgg tgcatgggtt tcgaagcagt ccagtcgcat ttctgcccgc ggtatccttg
     2161 aaagtaaatt cctggagctg aagcagaagt ttcaacaggg cgaagtgcca ttgccgagct
     2221 tttggggcgg ttttcgcgtc agccttgaac agattgagtt ctggcagggt ggtgagcatc
     2281 gcctgcatga ccgctttttg taccagcgtg aaaatgatgc gtggaagatt gatcgtcttg
     2341 caccctgaaa agatgcaaaa atcttgcttt aatcgctggt actcctgatt ctggcacttt
     2401 attctatgtc tctttcgcat ctggcgaaaa gtcgtgtacc ggcaaaggtg cagtcgttat
     2461 atacatggag attttgatgg caagcagtaa cttgattaaa caattgcaag agcgggggct
     2521 ggtagcccag gtgacggacg aggaagcgtt agcagagcga ctggcgcaag gcccgatcgc
     2581 gctctattgc ggcttcgatc ctaccgctga cagcttgcat ttggggcatc ttgttccatt
     2641 gttatgcctg aaacgcttcc agcaggcggg ccacaagccg gttgcgctgg taggcggcgc
     2701 gacgggtctg attggcgacc cgagcttcaa agctgccgag cgtaagctga acactgaaga
     2761 aactgttcag gagtgggtgg acaaaatccg taagcaggtt gccccgttcc tcgatttcga
     2821 ctgtggagaa aactctgcta tcgcggcgaa caactatgac tggttcggca atatgaatgt
     2881 gctgaccttc ctgcgcgata ttggcaaaca cttctccgtt aaccagatga tcaacaaaga
     2941 agcggttaag cagcgtctca accgtgaaga tcaggggatt tcgttcactg agttttccta
     3001 caacctgttg cagggttatg acttcgcctg tctgaacaaa cagtacggtg tggtgctgca
     3061 aattggtggt tctgatcagt ggggtaacat cacttctggt atcgacctga cccgtcgtct
     3121 gcatcagaat caggtgtttg gcctgaccgt tccgctgatc accaaagcag atggcaccaa
     3181 atttggtaaa actgaaggcg gcgcagtctg gttggatccg aagaaaacca gcccgtacaa
     3241 attctaccag ttctggatca acactgcgga tgccgacgtt taccgcttcc tgaagttctt
     3301 cacctttatg agcattgaag agatcaacgc cctggaagaa gaagataaaa acagcggtaa
     3361 agcaccgcgc gcccagtatg tactggcgga gcaggtgacc cgtctggttc acggtgaaga
     3421 aggtttgcag gcggcaaaac gtattaccga atgcctgttc agcggttcct tgagtgcgct
     3481 gagtgaagcg gacttcgaac agctggcgca ggacggcgta ccgatggttg agatggaaaa
     3541 gggcgcagac ctgatgcagg cactggtcga ttctgaactg caaccttccc gtggtcaggc
     3601 acgtaaaact atcgcctcca atgccatcac cattaacggt gaaaaacagt ccgatcctga
     3661 atacttcttt aaagaagaag atcgtctgtt tggtcgtttt accttactgc gtcgcggtaa
     3721 aaagaattac tgtctgattt gctggaaata atgcattaag tggaaagggg gagtgagaaa
     3781 tcactccccc tggtttttat acagggaaca tgatgaaaaa tattctcgct atccagtctc
     3841 acgttgttta tggtcatgcg ggtaacagtg cggcagagtt tccgatgcgc cgcctgggcg
     3901 cgaacgtctg gccgctgaac accgttcaat tttctaatca cacccaatac ggcaaatgga
     3961 ctggctgcgt gatgccgccc agccatttaa ccgaaattgt gcaaggcatt gccgccattg
     4021 ataaattaca cacctgtgat gccgtattaa gtggctatct gggatcggcg gagcagggtg
     4081 aacatatcct cggtatcgtc cgtcaggtga aagccgcgaa tccgcaggcg aaatattttt
     4141 gcgatccggt aatgggtcat ccggaaaaag gctgtatcgt tgcaccgggt gtcgcagagt
     4201 ttcatgtgcg gcacggtttg cctgccagcg atatcattgc gccaaatctg gttgagctgg
     4261 aaatactctg tgagcatgcg gtaaataacg tcgaagaagc ggttctggca gcgcgcgaac
     4321 tcattgcgca agggccacaa attgtgttgg ttaaacacct ggcgcgagct ggctacagcc
     4381 gtgaccgttt tgaaatgctg ctggtcaccg ccgatgaagc ctggcatatc agccgtccgc
     4441 tggtggattt tggtatgcgc cagccggtag gtgttggtga tgtgacgagc ggtttactgc
     4501 tggtgaaact gcttcagggg gcaacgctgc aggaggcgct ggaacatgtg accgctgcag
     4561 tctacgaaat catggtgacc accaaagcaa tgcaggaata tgagctgcaa gtggtggctg
     4621 ctcaggatcg tattgccaaa ccagaacatt acttcagcgc aacaaagctc tgaaatatct
     4681 catttcggcc cacaacgacc tgtgggccga tggctattac tttaagcctt ccgctgacag
     4741 cgcgtcttgt acttccggac gttcagccat acgttgcata aatgctgcaa tgtgctcaaa
     4801 cccttccaga ttcagtttca ccgcgtatgc ccagcgcaga accgtaaaca gataggcatc
     4861 agcaattgta aatctttgcc cgcagatcca gtgctcatcc ttcagtgcct cgttcacata
     4921 ttgcagcttc ttctccagct gcgcgcgaac tgtcggtttg tactcttccg gtgtatcagg
     4981 gcgaaacaga ggtgtgaaac ctttatgcag ctcggtggcg atgtaattca gccattcgat
     5041 ggttttatag cgggaaatac tgtttaccgg tgccagcaac tggcggtcgg ggacgctgtc
     5101 ggcaagatac tgcataatcg ctacgccttc cgtcagcaaa gtaccgtcat ccagcagcaa
     5161 tgcaggcacc tgccccttag ggttaacggc aaagtaatcg tcaccgtttt cgagacgttt
     5221 tttcattaaa tccacactga cgagggtaaa atcctttccg ctctcacgca gggtgatatg
     5281 ggaagcgaga gagcaggcac ccggtttgta gaacaatttc atcggtaact ccttttgtct
     5341 gtggtttaaa gtatggtagt gcgttgctga acaaaaaaaa agccgctaac gaaaagttag
     5401 cggcttgtaa aagagtttcc ctgaatttta cgctacggct gctttcgccg ctttgtctgc
     5461 agcgtcatcc tgcgtcatgc ggtgcagttt cggcgcggtc agcagcatca gtactgcaat
     5521 aacggcagta gcgacaccaa tctgcaagaa tacgcgacca tagacttcca gtgacatcag
     5581 cggatcggta acgttatccg gcacagccat catacccgca acataaccac caatcaggtt
     5641 tgcaccggca gtggtcagga accagctacc cataatgaag cccatcagac gctgcggaac
     5701 gagttgagca accattgcca gacccagacc agagatcatc agttccccga tgctctgcag
     5761 gccatagctt gcgaccagcc agcttacaga cacgatacca gcgtcagacg cgaatttcgc
     5821 acccagcggc agaatcagga acgcaccaga acacatcacc atgccgattg caaacttggt
     5881 tggcatcggc agggtatcgc ccatcttgtt atagatagcg gccagaatcg gactaccgat
     5941 gatgatccag aacgggttca gtgcctgata ctgctcaggt tctacggcca gacccagaat
     6001 ggagtgctca acgttacgaa tcgcaaagaa gttcagtgac gttggcatct ggctgtacag
     6061 cacgaagaag ataatggctt cgagcatcag gatgaaggca acgatcattt tacgacgcgc
     6121 agcacctttc atcgcgaagg cttctttacc gaagataacc acgataccga aggcaacaac
     6181 gcccagcgcc atacgcgcaa cttcctgatt gtgcagcagc caggtggcga tagcgatcag
     6241 tgccacaaca ccaataatgg tcagcagcag gttacggtag ttgataggct cgaagtctgg
     6301 ttttgaaccg tactgtttaa cccagcgttg gcagaaggcg aagttaacga tagtgatcag
     6361 caggcctaca acgctcaacg caaacgcaac actccagccg tatttcgcgg ccagccacgg
     6421 cgtagcaatc atagagaaga aagagccgat gttgacggac atgtagtaca tggtgaatgc
     6481 accgtccaga cgcgggtcgt ttttctcata gcatgtagaa agcagagaag acgggttagc
     6541 tttaaacagg ccgttaccga ccgcaatagc cgccataccc atataaacga taccggcgtc
     6601 gtgaccagac caggcaacca gagcataacc aatcgccagc acaatagcgc cgagcataat
     6661 tacgcgttta gtacccagta ccttgtcacc taaccagccg ccgatagcga ccagaccata
     6721 aaccagggca ctaaaggaag agaaaagggt gattgaatcc gcttcagaca tacccagttg
     6781 tttaaccagg taaacagcca taattccttg taggccgtaa taaccaaaac gttcccataa
     6841 ctcaatcgag aagatgagat agaacgcctt cggttgtttg aaagcgttca aactgacgct
     6901 ttcagttggt ttttggtttg cagtggacac ttttacctct ttttttacat cccatattaa
     6961 cgggggtgct ttctcgcgtt atgtataaaa aacatacgcg caattgttat agtgggaggg
     7021 gaaacggcag gtaatgttca ctatcttgca ctttcagaca agtcctttgt aataatctgt
     7081 tacatataac tgaggcgggg aattcaacca ttccgggaaa tgttatccag cgttaaattt
     7141 ttcaaaaaac gtaacggcag attttttcac tgcactgaga ggggggtgat ggcttaacgg
     7201 cgatatgttc tgctatttag cgtttttact agtgagatag tccagtttct gaaaaatagc
     7261 cagtgtaatg ttttgtaggt caatatttgt tgcgtgtgta aatttaaatg attttaacct
     7321 gattcttgca atgtttttac attgcgacag catcgtgatc ttgatcacga atgaatagaa
     7381 aatttcgcta ttaaaaaaac aattaacctg catttttggt tatccgcaaa cggattacct
     7441 gatgataacg ctaagcaatg gcatcattga cgttaaaaag atgttttaaa agaggataaa
     7501 gaaaggttat caatggggta atcggtgtta ccccttttct tcagatgtca actttctctt
     7561 tgtattcaca aagatcttca ataatacaag agccacagcg gggcttgcgg gcaatgcagg
     7621 tataacgccc gtgcaggatc aaccaatggt ggcagtcgac tttaaactct gccggaacca
     7681 ctttcagtag cttttcttct acctgttcga cgtttttccc cggcgcaaat tgagtacgat
     7741 tacaaacgcg gaaaatgtgc gtgtcgacag caatagtcgg ccagccgaat gcagtgttta
     7801 atacgacgtt ggctgtttta cgacctacgc cgggcagggc ttcaagcgca gcacgatctt
     7861 ccggaacctc gccattatgc tgctccagca agatacggca ggttttgatg atattttctg
     7921 ctttgctgtt ataaagccca atcgttttga tataggtttt caccccttca acgcccagtt
     7981 caagcatcgc tgcaggcgta ttcgccaccg ggtagagttt cgccgtcgcc ttattaacac
     8041 tgacatcggt cgcctgagcg gaaagcagta cggcaatcag caattcaaaa ggcgaactga
     8101 aattaagctc ggtggtggga tgaggattgt tctcacgcag gcgagtgagg atctccaggc
     8161 gttttgcttt gttcatcaga cattccctgt ttcaccgttt ggcaatgcac gttcggcagc
     8221 tgcttcagca cggcgctttt tcattctttc atcaatcagg tattttcctg ccagcatcag
     8281 tcccaggcca ataaatgcac ctggaggcag catcgccagc aggaaagggg agtcggtgtg
     8341 gaaaatttcc acgcgtaata cttttgccca gctacctaac agcgcatctg caccgtcaaa
     8401 caatgtgcca ttgccgataa tttcgcgtag tgaacccagc acgaacatgg cgcaggttgc
     8461 gcccatacca attgaaaagc cgtccagtgc cgaaagcgcc ggaccttttt tggcggcgaa
     8521 ggcttcagcg cggcccacaa cgatacagtt agtgacaatc agcggaataa aaatccctaa
     8581 tgattgatat aggccaaagg cgtaggcgtt gatcagcatc tgtacagcgc tgaccaccga
     8641 ggcgatgatc atcacgtaaa tgggaatgcg gatctcggct ggcgtccagt gacgcagcgt
     8701 cgaaatggtc aggttggtca gcgtcagtac cagcgtagtc gcaagtccta aacccagagc
     8761 gttagtggca gtggacgtga ccgccaacag aggacaaagg ccgagcaact ggaccagagc
     8821 agagttgttt ttccacaacc cctgaacaat aacgtcttta atttcgctca cgctttattc
     8881 tccacaggca ggaagttgag aaagttgtgc cggtaacgtc tgagcgtaca atccggcgcg
     8941 ttttaccgca ttaaccaccg cgcggggagt aatcgtcgcg ccggtgaact ggtcgaaatc
     9001 accaccatct ttcttcaccg cccagtgcgc atcatccgca ccactgattt ttttacccgc
     9061 aaaatgggtg atccagtcag aaaggcgcag ttcgatttta tcgccaagcc ctggcgtttc
     9121 gtggtgctct gtcacgcgcg tgccaagtac cgtgccgtta aaatcggctc ccaccagcag
     9181 ctgaatcgca ccggaatagc catctggcgc ggttgcttcc agaacggcgg ctaccggttt
     9241 gtcatcctgt ttggcgatgt aaacccgatg ctcaccttta cctaactctg gcgcagttac
     9301 cagatagcaa ctctgtgcca gcgcattgtt atagcgttcg gctggcagca cctgatcaaa
     9361 taatgccttt tgttgcagac tggcctgttc agcaatcgtc gttttggtca tctggttgat
     9421 ggccgcagtt aaccctgttg aacccgctgc aaatagcgct aacgtaatgc cgtgttttcg
     9481 gatagttttc agcatggttt accctttgcg atggccgtag acgcgcggac gcgtgtagta
     9541 atcgatcaga ggaaccgtga tgttcgccag caggacggca aaagccacgc cgtcaggata
     9601 gccgccgaaa ctgcggatca accagactaa taagcccgcc agcgcgccga aaataagacg
     9661 accacgattg gtcgtagaag cggtaaccgg gtcagtcaaa ataaagaatg cgccgagcat
     9721 ggtcgcacca gacagcagat gaatttgcgg tgctgccagt gtttctggtg agaacaacca
     9781 gcccaacgtt gcgcataacg ccagcgttac taagaagctg aggggaatat gccagcgaat
     9841 cgctttctgc catagcaacc acaggccgcc agccagccag gcgagattta cccattgcca
     9901 accagcgccc gccagaatac cgctgtagat cggatattgc ataatctgtt caaccgaatg
     9961 accggcacgg acagaggttt taaatgtatc cagcggtgtc gcctgactaa tgccatcaat
    10021 acctaagcgt agtgtgttca tatcaccacc actggcggta tgtccgctga aaataacctg
    10081 tatggcgtcg ataaaaccag ggatgttgac cgcaatttca tgtggcggta accagctggt
    10141 catctgcacc gggaaggaga tcagtaagac cacataacca atcattgccg gattaaacgg
    10201 gttttgcccc agaccgccat acaactgttt agcgatgatc accgcaaaca ccgtacccag
    10261 cacgaccatc caccatggcg cgaggggggg aatacttacc gccagcaata agcctgtcag
    10321 caatgctgag ttatctttca acgttgcggc taccgactgc ttgcgtagtt tgagtacgag
    10381 agcttcggct aacagagcac taaccgatgc caacaggatc tgaacgagag taccccaacc
    10441 aaaaaaccac agttgcgctg cgattcctgg cacggctgcg agcaacacca acagcataat
    10501 gcgcgatgtc tggcgctggt tatgggtata aggggagcta gctattctga ataccattta
    10561 gtcctcgttt acaaccttct gctgggcggc ttttttggcc tgagcgcggg caatagccgc
    10621 ggcaactgcc gctttgcgcg gatcaacctg ttcttctggt tctgcgttag tctgttgctg
    10681 ttccagcttg cgcgctttgg cacgggcaat agcggcttcg acggcggctt tgcgcggatc
    10741 aacctgttct tctggttccg cattagcctg ttgctgttcc agtttgcgcg ctttggctcg
    10801 ggcaatagcg gcttcgacgg cggctttgcg cggatcaacc tgttcttctg gttccgcatt
    10861 agactgttgc tgttccagct tgcgcgcttt ggcacgggca atagcggctt cgacggcggc
    10921 tttgcgcgga tcgacctgtt cttctggttc cgcattagcc tgttgctgtt ccagcttgcg
    10981 cgctttggcg cgggcgatag ctgcttcaac ggcagtttta cgtggatcag caacggttgc
    11041 tgcgtcgtta gtttgctgca gttctgcctg tttcgctctg gcttgcgctt tacgggcttc
    11101 ccgtgctgca ataattgcac tgttatccgg gcgttcgccc gctttaatca caataggctg
    11161 tgtagcctgg gcctgtttct ctttcacccg cgccagagcg gcagcaatcg catctttatc
    11221 tttggctgca ggttgaacgg ctgcgctctt atgtcgttca aggcgagccg ctttttcgcg
    11281 ctccagacga gcctggcgcg cttcgaaacg cgctttggct tctgcggcgc gcttttcttc
    11341 ctgacgaata gccgcaattt cagctttttc ctgacggaaa tattgcacca ggggaatatt
    11401 gctcgggcaa acccacgcgc aagccccaca ttcaatgcaa tcagcaatgt tatgcgtggt
    11461 agctttatcg tgttgctgac ctttgctgaa ccagtacaac tgttgcggca aaagatccgc
    11521 agggcaggcg tcagcacagg cgctacaccg gatgcagctt tgttcttcct gtggttcgcc
    11581 aagttcattg gcagagggag ccaacagaca gttggtaatc tttacgaccg ggacatccag
    11641 ccatggcaag gtaaagccca ttagcgggcc acccataatc accatttgat cggcagaggg
    11701 gcagaatccg gcatcattca ataaatgacg cactggcgtc cccagccgtg cccagacgtt
    11761 gcccgggcga gcgattgctt cgccagtcag ggttacaaca cgctcggtaa tcggctcgcc
    11821 atcaataacg gcacgtttca ctgcataagc agtgccgacg ttttgcatta atacgccgat
    11881 atcggatgaa cgcccgccat gtggaacctg cttcccggtc agaatgtagg ttaattgttt
    11941 agcaccgcca gaaggatatt tggttggaat cacccgcaga gaaatatcgt tagagtccgc
    12001 cagcaccgcg cgcagcatgg aaatcgcctg cggtttgtta tcttcaatgc cgataagaat
    12061 ttcgcgtggc tgcagaatat gcgcaagaat gcgaataccc tctacgacct gagccgcgca
    12121 atcctgcatc aaacggtcat cggcggtaat gtacggctcg cactcagccg cgttgataat
    12181 caacgtttca atcttatctc cgccaccctg caatttaacg cctgtcggga atcctgcacc
    12241 gcccagcccg gctacaccaa actgatgtat gcgctcgatt aactcttcgc gactgcgagt
    12301 gcgataatcg gcccagccgt cgcgcgggat ccagcagtct tcaccatcgg catcaataat
    12361 cacgcttaat tcagctaaag ctgaaggatg agccgtagag tggggcgcaa tagccgtaac
    12421 ggtacccgag gtgggcgcgt gaacaggcag cattttgccg cgaccacggg taagcggctg
    12481 gccgcgcaat actttatcgc cgacgctaac gcacaactca ccttcagcgc caatatgctg
    12541 tttcagtgga ataacaaaac gctgcgccag tggtacctgg cgcagtggtg taccgttgga
    12601 ctgggttttc atctccggtg gatggatgcc gccgttgaaa tcccagattt tattttttct
    12661 gaatgcagag aataacttaa gcatggtgtt ccacgggaat gatacgcacg ggaatggtgt
    12721 tcagatccca tttccaggag tcaggtgttt ctgcgaccgg ttgcaacgag atgcagtgcg
    12781 tcgggcacgg atcaacacat aaattgcagc ccgtacagag atcactcatt accgtatgca
    12841 tggcacgggt agcgccaacg atggcgtcta ccggacacgc ctgaatacat ttagtgcagc
    12901 caatacagtt attttcatca ataaccgcca ccatccgcgc aggcgttagc tcttgcgctt
    12961 cgccatccag cggctgcggc tcgacattaa gcaactcggc aatttttagc atcacagctt
    13021 cgccacctgg ggcgcaacgg ttgatttttt caccgttaca gctgatggct tccgcgtagg
    13081 ggcgacagcc gggataaccg cactgaccac actggctctg cggtaagatt tcgtcaattt
    13141 tctcaacgac cggatcgtct tccaccgcaa aacggcggga ggcataaccc agaatggcgc
    13201 caaacgccag gcccagcagg ctcacggcgg caacggcaat ccagatagca ttcattacaa
    13261 cttcaccaaa ccactaaagc ccataaaggc cagagacata agacctgcgg taattaacgc
    13321 aatggcatta ccgcgaaaag gtgccgggac atcagccaca gcaaggcgtt cgcggatggc
    13381 tgcgaagaga accatcacca gcgaaaaacc gacagcggcg gaaaaaccgt acagcgccga
    13441 ctgcaagaaa ttgtgcccga gattgatatt cagcaacgcc acgccgagca cggcacagtt
    13501 ggtggtgata agcggcaaaa aaatccccag caaacggtaa agcaccgggc tggttttgcg
    13561 caccaccatc tcggtgaact gcacgaccac agcaatcacc agaataaatg ccagggtgcg
    13621 caagtaaatc agattaagtg ggatcaaaat ccacgtatcg ataagccagg cgcaaataga
    13681 cgccagcgtc atcacaaacg ttgttgccag ccccatgccc atcgcggttt ccagtttttt
    13741 ggaaaccccc ataaacggac agagaccgag aaacttgacc agtacaaagt tattgaccag
    13801 tacagttccg acaaacagta acaggtagtc agtcattttt cggcctgaaa taaaaaagcc
    13861 gcctattatc cgttaatcca gagcaggcga caacaggtta actgtaaggt tattacggat
    13921 tcacaaacgt cgcttttacc cgcgacgagc gtttgaaata ggggacgatc aatgcggttg
    13981 ccaacaaagt aaacagcaac tggcgaacgg ctatgccatc ttccacgggt gaaaaagcga
    14041 atgcttttac tgccagcaat acggaaataa gcagccagat aatatagtgt ttaggaacac
    14101 aacgccgacg tttaaagaaa gcgatggtca accacagtgt gtaataccac atggcgatag
    14161 cggtaataaa agaaacgccc cataagattt gtgtcgtcag tgcttgtccg ccgagcgttt
    14221 gaaatgtttg cggagaagat aacgcagcgg tatacagcaa caacgccagc gtcgtactca
    14281 acagagcgac taaaagccag gccaacgggc caagcaacca gcctccaata cgttgtggcg
    14341 ttgtggtggt cataggttct ccagaatagc gacgcaaaca ggcaaatcta attttagggg
    14401 cgttattata aggcttttat aaccgatcgc tactttcttt attggacata gtgccagacg
    14461 gacttcggta cctggccgag gtcaaacaaa cgcccgccag aaaccagctc tgcgcgacgg
    14521 tgatcggcag cacgatacat attgatcagt tcctgatcgt ccgtcagggt gtaattaaga
    14581 tggtcgtaga gtttttccag actttcgagt gaactgattt tgcgaaattt taataagtag
    14641 tcctgaacag tcataaaagg tccataaaat aattaatacc aaaggggtag gtattattag
    14701 tgatgataaa atttcaccaa aagtgaacgc ttgtgttatt acaataataa gaatgcgctg
    14761 atggcaaata ttcagggatg aaaaactgct ctgaaaagag taacgcgctt tactatccct
    14821 gacaatcacc aacaacatcg aacaagataa taaattcctg gtttaatatc cgacaagtga
    14881 aaacatgcac ccggacgggc agcatgtcgc tccacaagtg cagagcttac ttgtgttgta
    14941 ccgaagcact ctgttcaggt ggctgatagt tgtcaatgtg actcgccacg ccaagaagaa
    15001 tgactgaaac gacaagaacg atccaacctg ttaattcaat aagacgattc attacagccc
    15061 acatctctct tgattgatcc attaacttca gggggtaaat gttacttagc aataatagct
    15121 ccagcaagat ttttactgag gtttttgcga tatttagctt ttgtcgttgg aaaattcgct
    15181 atttttttga cttaagttaa acaacatccc ttattgctgg caggttatta aactgttgag
    15241 cgtgggtaaa ggatagtgtc aaatagccat catacttcaa tgagaggcaa tgacatgagc
    15301 gacaacatcc gtgttgggtt gattgggtat ggttatgcga gcaaaacctt ccatgcgccc
    15361 ctgattgcgg gcacgcccgg gctggaactg gcggtaatct ccagcagcga tgaaacaaaa
    15421 gtaaaagccg actggccaac ggttacggtt gtctctgagc cgaagcatct gtttaacgat
    15481 cccaacatag acctgattgt cattcctaca cccaacgata cccatttccc gttagccaaa
    15541 gcggcgcttg aggcgggtaa acatgtggtc gttgataaac cctttaccgt gacactgtca
    15601 caagcgcgag agctggatgc gctggcaaaa agcctggggc gtgtgctgtc tgtattccat
    15661 aaccgtcgct gggatagcga tttcttgacg ctaaaaggtt tactcgcgga aggcgtgctg
    15721 ggtgaagttg cttactttga gtctcatttt gaccgcttcc gtccgcaggt gcgcgatcgt
    15781 tggcgtgaac agggcggccc aggcagcggt atctggtacg atttagcacc acatcttctt
    15841 gatcaggcca ttacgctttt tggtttaccg gtcagcatga cggtagattt ggcacagtta
    15901 cggcccggag cgcagtcgac cgattatttc cacgccatct tgtcctatcc acagcggcga
    15961 gtcattttac acggtaccat gctggcagcc gctgagtcag cacggtatat cgtgcatgga
    16021 tcccgaggca gttatgtgaa atatggcctc gatccacagg aagaacgtct gaaaaatggc
    16081 gagcgtctac cgcaggaaga ctggggctac gatatgcgtg atggcgtact tacccgcgtg
    16141 gaaggtgagg aacgtgtcga agaaacgctg ttgacggtgc ctgggaatta tccggcttac
    16201 tatgcggcta ttcgtgatgc gttaaatggc gatggtgaaa atccggttcc ggcaagccag
    16261 gcaatccagg taatggagtt gattgagctg ggcatcgaat ccgccaaaca tcgcgcgact
    16321 ttgtgccttg catgaaaaat ggatggtgca atttgcacca tccattttgt tacttcgcgg
    16381 cgactttttc tcgcagtgcg cgtttttcct cagcgctgag gaaagccatt tccagaccat
    16441 taatctgtgt ctggcggatt tgctcgcggg ataacccagc agctggcgcg gcaacggtat
    16501 attcgtgaat gatatccact ccctgtacgc cgggatcgtc agtgttaatg ctggcacgaa
    16561 tgccatgctc aaggaacgtt ttcagcggat gtgcagccag ctctgctacg gtgctggtct
    16621 gaatattgga ggtcagacag gattcaatac caatttgttg ctcggcgaga aaatccatca
    16681 gcgcccgatc ttcaatggct tttacgccat gtccaatacg ctccgcaccc agttcacgaa
    16741 tcgcctgcca gatgctttcc ggcccggcag cttcgcctgc atggacggta atatgccagc
    16801 ccgcatcacg cgcgcggttg aagtgagaaa ggaacagact tcccgggaaa ccaagttcat
    16861 caccggctaa atcaagtgcg gtaatctggt cacggtgggc taaaaaggcc tccagctctt
    16921 gctgacaggc ggcttcgccg aaggtccggc tcataatgcc gataagcttc gcctgcacac
    16981 caaaggtgcg gcaaccttca cgtacgccat cgatcaccgc ttcgacaaca cccgctacag
    17041 gcagctgatg tgccattgcc atgtagcctg gtgaaaaacg cagctcgaca tagtgcaggc
    17101 cgtgacgggc tgcatcttca atgttttcaa atgccacgcg gcgacaggca tcaagagagg
    17161 cgagaacttt aacgccccag tcaagtttgg ccagaaagct caccagatcg ggttcgttgg
    17221 caatgacctg aacgtgggga atcagtgttt ccagggattg tgcaggaagc gagatattat
    17281 actggcggcc aagttcaaga atggtctggg gacgaatgtt gccatcaagg tggcgatgga
    17341 tatcagttaa tggcagggtg gtatcaatca tggtcgcact ctttttctgg atataaaagt
    17401 gcgcaccatt ataaaaacaa agcagggtaa aaatccacga ttgcgcaacg attttttacg
    17461 ttgcgcaatt ggggttaacg aacagcgcgg atggcgttaa ttaatccagc cacacctttt
    17521 tccagtttcg aacgtgggca gccggcattg agacggacaa aaccacgacc ttcttcaccg
    17581 taggtatacc ccggcatgat cgcgactttt tcttgttcga taagtgcttt ttgcaacgcg
    17641 ttgtcgtcaa tattcaacgg acgtaaatca agccatgcca gataagtgga ttgcgggatc
    17701 tgccagttga gttcaggaaa tgtggcgttc attttatctg cgatatacgt caggttatct
    17761 ttcagataga tgcgtaaggc atccagccac ggtgcacctt gctgataggc ggcgatatgg
    17821 gcagttaacg ccagtaccga aggggaagaa agcccatcac gtcctttcag tgccgataaa
    17881 taggcatcac ggctgctgct attttctata atcccgtaag caccggtcag cgcgggaata
    17941 ttgaaacttt tcgatcccga cgtcagcaac gcccagtctc cccgcgccac attactccag
    18001 ggaatatgcg gctgctcgcc ccagaccata tccatatgga tttcatcgga aataacccgc
    18061 acaccatgac gctcgcatag gtcagccatg atctccagct catcgcacgt ccacacttta
    18121 ccggtaggat tctgtgggct acacaggagc ataattttac attctggttt cgccaacacg
    18181 gcttccagct tgcccatatc gcaaaaccag ccgtcagcct gcttctctaa agcaacgggc
    18241 attactgtgc gctggttacc ttcaatggcc ttgtaaaatg cgtcataggc gggcgtgtgg
    18301 atcaccacgc cttcacctgt ttcagaccac tgacgaatca gttctgaaac catatagatg
    18361 acagaagggc catacaccac cgtctgcgta tcaattgcgg tgtaatgctg ggtggaaaac
    18421 cagtgggcaa tagccgcgag aaactcatcg tttttccagc ggctgtagcc aaatacgccg
    18481 tgcatcaggc gctgattcag cgcctcgata atgcaggggg cagtggcaaa atccatgtct
    18541 gaaatcgtga acggtaacag gtcagcagtg ccgaaacggt cagcgacata atcccactgc
    18601 gtgcaccatg tgccatgacg atccacgacc tttgaaaaat cgaacatatc gtatccttat
    18661 gcctggacag tatgcatcag accggccatt tcatctttta ctgactgaac ttgtggcccg
    18721 ataacaacct gcaggttatg ttgattaagt tgtactacgc caattgcccg attgtccttc
    18781 agtgcctgca cattaacaag cgacatatct ttcacagaca aacgcagacg ggtaatgcag
    18841 ttatcgaggc tgacaatatt gtcggcaccg cctaatgctt cgaggattgc aggaacgttg
    18901 taacctgatt tacccggcgc accggcaacg gctttttcga ttgagctggc aacttcgcta
    18961 tcgcgccccg gggttttcag attgaagcgg gtgatagcga aacggaagat gacgtagtaa
    19021 acgacaaacc agattgccgc caccactggc accatgtacc acttggttga cagaccatgc
    19081 aaaataccga acaccacgaa gtcgatgata ttgccgtcgg tattaccgat ggtgacgccg
    19141 agcacagaca tgacggtgaa gccgaggccg gttaacagcg cgtggatgac atacagaact
    19201 ggcgctacga acaggaacag gaattccagc ggttcggtag tgccgccaac gacgcaggcg
    19261 atcaggccag aaatcagcag acctttaatt ttatggcgat tttccgggcg cgcgcagtga
    19321 tacatagcta acgctgcacc tggcagaccg ccgagaaacg caggcatttt accttgcgaa
    19381 aggaaacgcg tggcgctttc agaaaaaccg tgagtggtcg ggcaattcaa ttgcgcctgg
    19441 aagatggtca gtgcgccgct gacggtttga ccgcagactt cctgcgtgcc gcctgcgtcg
    19501 gtaaagcgaa ttaatgccac cagaatgtga tgcagaccaa acggcaacag cagacgttca
    19561 ccggtaccaa acagcatcgg tccgaaatca cccgcgctgt ttatcatatg gcccaagccg
    19621 ctaataccca tggcgaaaat cggccagact aatggaatca ccaggccgac aaggcccatc
    19681 accagcgagg agataattgg tacgaagcgc gtaccgccga agaatgccag cgcatccggc
    19741 aggcggatat tatggaaacg ctcatgcagc atccagacga taataccggc gatcaccgca
    19801 ccgaggatcc cggtatcgat cgactggatc ccaagaatgc tctggatgtt attggctttc
    19861 agaaccgcgg catccgtggt tggcagaatg cctttattgg tcaaccagaa gtttaccgcg
    19921 aggttcatta ccgcataacc gacgaagcca gcgaatgccg ctacgccttt gttttcgcgt
    19981 gccaggccca gcgggatggc gatacagaac atgacaggca ggaaactaaa agcaaacgag
    20041 ccaatcttac tcatccaggt aaagatagcc tgcaacacgg ggttgcccag gaccgggatt
    20101 agggtgatga catcgtggct actaagggaa ctgccaatgc cgagcataat gccgcagaac
    20161 gacaataatg ccacgggtaa catgaaggtt ttgcctaact gctggaagaa ctcccacagc
    20221 gtgactttcg gtgctgtttt cgccgtcata aaacgactcc tcgtagagaa caatctggat
    20281 tcagtaaatt gcgcgagaag ataaaacgtt ttatcaaatt ttagtgaggc ataaatcaca
    20341 ttacgcaacg ataatagcgg gtataagata aataaaaggt aaaacgtttt atctgtcaca
    20401 taatcaggga gtaggtcatc tgcatggcta ccgccaaaaa aataaccatt catgatgttg
    20461 cgctggctgc gggcgtgtcg gtaagtaccg tttcgctggt gcttagtggc aaagggcgaa
    20521 tctctaccgc cacaggagaa cgcgttaacg ccgccattga agagctggga tttgtgcgca
    20581 atcgccaggc gtcggcgctg cgcggcggac aaagcggcgt cattggtttg atcgtccgtg
    20641 atttatctgc gccgttttac gccgaattga cggccggatt gacggaagct ctggaagcgc
    20701 agggacggat ggtttttttg cttcacggcg gtaaagacgg cgagcagctg gcacagcggt
    20761 tttcactgtt actgaatcag ggtgtcgatg gtgtggtaat agccggggct gcaggaagca
    20821 gcgatgacct gcgacggatg gcagaagaaa aagctatccc ggtgattttc gcttcccgtg
    20881 ccagttatct tgatgatgtt gatacggttc gcccggacaa catgcaggct gcacagttgt
    20941 tgacggagca tctcattcgc aatgggcatc agcggatcgc ctggctggga gggcaaagtt
    21001 cctcattaac ccgtgcagaa cgggtggggg gctattgtgc aactctacta aaatttggcc
    21061 tgccgtttca cagcgattgg gtgttggagt gcacttccag ccagaagcaa gcagcggaag
    21121 ctatcacggc gcttttacgt cataacccga ccatcagtgc cgtggtttgc tataacgaaa
    21181 ctattgcgat gggggcatgg tttggtttgc tgaaagcagg gcggcaaagc ggggaaagcg
    21241 gagtcgatcg ttactttgag caacaggttt cgctggcggc atttaccgat gcgacaccaa
    21301 ccacacttga tgatatacct gttacctggg ccagcacgcc agcgcgggaa cttggtacca
    21361 cacttgcgga tcgcatgatg caaaaaatca cccatgaaga gacgcattca cgcaatctta
    21421 ttattcccgc ccggctcatt gcggcgaaat aatcctctct ttatctgcta tacctggtag
    21481 tgtcctttcc tcaaggttaa tgataaaaaa tgtggcacac aggcggtgcc ttttattttt
    21541 actgaagttc acaggaggtt tatgtgttta attctgacaa cctgagactc gacggaaaat
    21601 gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt acattcgcga
    21661 cagctggcgc atctgtggtg gtcagtgata ttaacgccga cgcagctaac catgttgtag
    21721 acgaaattca acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt acttccgaac
    21781 aggaactctc tgcactggca gactttgcta tcagtaagct gggtaaagtt gatattctgg
    21841 ttaacaacgc cggtggcggt ggtcctaaac cgtttgatat gccaatggca gattttcgcc
    21901 gtgcttatga actgaatgtg ttttcttttt tccatctgtc acaacttgtt gcgccagaaa
    21961 tggaaaaaaa tggcggtggc gttattctga ccatcacttc tatggcggca gaaaataaaa
    22021 atataaacat gacatcctat gcatcatcta aagctgcggc cagtcatctg gtcagaaata
    22081 tggcgtttga cctgggtgaa aaaaatattc gggtaaatgg cattgcgccg ggggcaatat
    22141 taaccgatgc cctgaaatcc gttattacac cagaaattga acaaaaaatg ttacagcaca
    22201 cgccgatcag acgtctgggc caaccgcaag atattgctaa cgcagctctg ttcctttgct
    22261 cgcctgcagc cagctgggta agcggacaaa ttctcacagt ctccggtggt ggggtacagg
    22321 agctcaatta atacactaac ggaccggtaa acagccgtac gtgttgttta ccgggatagc
    22381 gtgtgcaatt catcaacgtc tctgctaaac aactggcagc caaatcacgg ctattggtta
    22441 accaatagct ggatgaaaag catacgaata gagtgtgcct tcgcactatt taacagcaat
    22501 gataggcgct cacctgacaa cgcggtaaac tagttattca cgctaactat aatggtttaa
    22561 tgatggataa catgcagact gaagcacaac cgacacggac ccggatcctc aatgctgcca
    22621 gagagatttt ttcagaaaat ggatttcaca gtgcctcgat gaaagccatc tgtaaatctt
    22681 gcgccattag tcccgggacg ctctatcacc atttcatctc caaagaagcc ttgattcagg
    22741 cgattatctt acaggaccag gagagggcgc tggcccgttt ccgggaaccg attgaaggga
    22801 ttcattttgt tgactatatg gtcgagtcta ttgtctctct cacccatgaa gcctttgggc
    22861 aacgggcgct ggtggtcgaa attatggcgg aagggatgcg taacccacag gtcgccgcca
    22921 tgcttaaaaa taagcatatg acgatcactg aatttgttgc tcagcggatg cgtgatgccc
    22981 agcaaaaagg cgagataagc ccagacatca acacggcaat gacttcacgt ttactgctgg
    23041 atctgaccta cggtgttctg gccgatatcg aagcggaaga tctggcgcgt gaagcgtcgt
    23101 ttgctcaggg attacgcgcg atgattggcg gtatcttaac cgcatcctga tcctctcatt
    23161 tcgcgggctg gtgataactg tgcccgcgtt tcatacttta atttccctgt acaaaataca
    23221 atctccccgg gatcggagaa ttcccccccc aaaatattca ccgtagccat atgtcatgag
    23281 agtttatcgt tctcaagata ctcgaacgaa cgttcgattg cttattttat ggcttctgtc
    23341 aacgctgttt taaagattaa tgcgatccat atcacgctgt gggtattgca gtttttggtt
    23401 ttttgatcgc ggtgtcagtt ctttttattt ccatttctct tccatgggtt tctcacagat
    23461 aactgtgtgc aacacagaat tggttaacta atcagattaa aggttgacca gtattattat
    23521 cttaatgagg agtcccttat gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa
    23581 ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gcggaattga tcagcgttgg
    23641 tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt taacgatcag
    23701 ttcgccgatg cagatattcg taattatgtg ggcaacgtct ggtatcagcg cgaagtcttt
    23761 ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac
    23821 ggcaaagtgt gggtaaataa tcaggaagtg atggagcatc agggcggcta tacaccgttt
    23881 gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tgcgtatcac cgtttgtgtg
    23941 aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga cgaaaacggc
    24001 aagaaaaagc agtcttactt ccatgatttc tttaattatg ccgggatcca tcgcagcgta
    24061 atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg
    24121 caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga tgtcagcgtt
    24181 gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag cgggactttg
    24241 caagtggtga atccacacct ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc
    24301 acagccaaaa gccagacaga gtgtgatatc tacccgcttc gcgtcggcat ccggtcagtg
    24361 gcagtgaagg gcgaacagtt cctgattaac cacaaaccgt tctactttac tggctttggt
    24421 cgtcatgaag atgctgactt gcgtggcaaa ggattcgata acgtgctgat ggtgcacgac
    24481 cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta cccttacgct
    24541 gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga aactgctgct
    24601 gtcggcttta acctctcttt aggcattggt ttcgaagcgg ccaacaagcc gaaagaactg
    24661 tacagcgaag aggcagtcaa cggggaaact cagcaggcgc acttacaggc gattaaagag
    24721 ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc caacgaaccg
    24781 gatacccgtc cgcaaggtgc acgagaatat ttcgcgccac tggcggaagc aacgcgtaaa
    24841 ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat
    24901 accatcagcg atctctttga tgtgctgtgc cttaaccgtt attacggttg gtatgtccaa
    24961 agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc ctggcaggag
    25021 aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc cgggctgcac
    25081 tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac
    25141 cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt
    25201 gcgacctcgc aaggcatatt gcgcgttgga ggtaacaaga aagggatctt cactcgcgac
    25261 cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat gaactttggt
    25321 gaaaaaccgc agcagggagg caaacaatga atcaacaact ctcctggcgc accatcgtcg
    25381 gctacagcct cggtgacgtc gccaataact tcgccttcgc aatgggggcg ctcttcctgt
    25441 tgagttacta caccgacgtc gctggcgtcg gtgccgctgc ggcgggcacc atgctgttac
    25501 tggtgcgggt attcgatgcc ttcgccgacg tctttgccgg acgagtggtg gacagtgtga
    25561 atacccgctg gggaaaattc cgcccgtttt tactcttcgg tactgcgccg ttaatgatct
    25621 tcagcgtgct ggtattctgg gtgccgaccg actggagcca tggtagcaaa gtggtgtatg
    25681 catatttgac ctacatgggc ctcgggcttt gctacagcct ggtgaatatt ccttatggtt
    25741 cacttgctac cgcgatgacc caacaaccac aatcccgcgc ccgtctgggc gcggctcgcg
    25801 ggattgccgc ttcattaacc tttgtctgcc tggcatttct gataggaccg agcattaaga
    25861 actccagccc ggaagagatg gtgtcggtat accatttctg gacgattgtg ctggcgattg
    25921 ccggaatggt gctttacttc atctgcttca aatcgacgcg tgagaatgtg gtacgtatcg
    25981 tggcgcagcc gtcattgaag atcagtctgc aaaccctgaa acggaatcgc ccgctgttta
    26041 tgttgtgcat cggtgcgctg tgtgtgctga tttcgacctt cgcggttagc gcctcgtcgt
    26101 tgttctacgt gcgctatgtg ttaaatgata ccgggctgtt tactgtgctg gtactggtgc
    26161 aaaacctggt cggtactgtg gcatcggcac cgctggtgcc ggggatggtc gcgaggatcg
    26221 gtaaaaagaa taccttcctg attggcgctt tgctgggaac ctgcggttat ctgctattct
    26281 tctgggtttc cgtctggtca ctgccggtgg cgttggttgc gttggccatc gcttcaattg
    26341 gtcagggcgt tactatgact gtgatgtggg cactggaagc tgataccgta gaatacggtg
    26401 aatacctgac cggcgtgcga attgaagggc tcacctattc actattctca tttacccgta
    26461 aatgcggtca ggcaatcgga ggttcaattc ctgccttta
//
LOCUS       DAFNBJ010000060        25996 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000060 DAFNBJ010000000
VERSION     DAFNBJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.060"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            182..1234
                     /locus_tag="L2X49_002338"
     CDS             182..1234
                     /locus_tag="L2X49_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HBN4797418.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1893..2327)
                     /gene="pcoE"
                     /locus_tag="L2X49_002339"
     CDS             complement(1893..2327)
                     /gene="pcoE"
                     /locus_tag="L2X49_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system metallochaperone PcoE"
                     /protein_id="HBN4797419.1"
                     /translation="MKKILVSFVAIMAAASSAMAAETMNMHDQVNNAQAPAHQMQSSA
                     EKSAIQGDSMTMMDMSSHDQAAMSHDMMQNSNSAAHQDMAEMHKKMMKAKPGATNETA
                     KSFSEMSEHEKAAAVHEKANNGQSSVVHQQQADKHRSQITQN"
     gene            complement(2545..3945)
                     /gene="pcoS"
                     /locus_tag="L2X49_002340"
     CDS             complement(2545..3945)
                     /gene="pcoS"
                     /locus_tag="L2X49_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance membrane spanning protein
                     PcoS"
                     /protein_id="HBN4797420.1"
                     /translation="MRFKISLTTRLSLIFSAVMLTVWWLSSFILISTLNGYFDNQDRD
                     FLTGKLQLTEEFLKTETFRNKTDIKSLSEKINDAMVGHNGLFISIKNMENEKIVELYA
                     KNSVVPAVLLNKSGDILDYMIQTEENNTVYRSISRRVAVTPEQGKSKHVIITVATDTG
                     YHTLFMDKLSTWLFWFNIGLVFISVFLGWLTTRIGLKPLREMTSLASSMTVHSLDQRL
                     NPDLAPPEISETMQEFNNMFDRLEGAFRKLSDFSSDIAHELRTPVSNLMMQTQFALAK
                     ERDVSHYREILFANLEELKRLSRMTSDMLFLARSEHGLLRLDKHDVDLAAELNELREL
                     FEPLADETGKTITVEGEGVVAGDSDMLRRAFSNLLSNAIKYSPDNTCTAIHLERDSDC
                     VNVMITNTMSGQVPANLERLFDRFYRADSSRFYNTEGAGLGLSITRSIIHAHGGELSA
                     EQQGREIVFKVRLLMD"
     gene            complement(3942..4622)
                     /gene="pcoR"
                     /locus_tag="L2X49_002341"
     CDS             complement(3942..4622)
                     /gene="pcoR"
                     /locus_tag="L2X49_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     PcoR"
                     /protein_id="HBN4797421.1"
                     /translation="MQRILIVEDEQKTGRYLQQGLVEEGYQADLFNNGRDGLGAASKG
                     QYDLIILDVMLPFLDGWQIISALRESGHEEPVLFLTAKDNVRDKVKGLELGADDYLIK
                     PFDFTELVARVRTLLRRARSQAATVCTIADMTVDMVRRTVIRSGKKIHLTGKEYVLLE
                     LLLQRTGEVLPRSLISSLVWNMNFDSDTNVIDVAVRRLRSKIDDDFEPKLIHTVRGAG
                     YVLEIREE"
     gene            complement(4677..5606)
                     /gene="pcoD"
                     /locus_tag="L2X49_002342"
     CDS             complement(4677..5606)
                     /gene="pcoD"
                     /locus_tag="L2X49_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance inner membrane protein PcoD"
                     /protein_id="HBN4797422.1"
                     /translation="MNDLIMIVIRFLLYLDLMVIFGLPFFQIYGISGVRHETYNLTNF
                     RSFITFAVVTGIILTGINMLLVSNAMSGVTDLRELSIHVIEMVIEETDVGISWIVRLC
                     ALFTTLGALFLYTNKRVLSCLLMTMSGGVALATLAWGGHAVMHDGLHYYLHLLSDLTH
                     LGAAGAWTGALVAFAILLMRRNEHNAQSVIVISDSLAKFATAGTVIVVALILSALVNY
                     LYIAEGNLTPLFNSSWGRILLAKTALFVLMLLLAAANRFHLGPRLEVMVREGNYDRSV
                     ALMRNSILTEFVVAIIILGAVAWLGMLAPSQVS"
     gene            complement(5611..5991)
                     /gene="pcoC"
                     /locus_tag="L2X49_002343"
     CDS             complement(5611..5991)
                     /gene="pcoC"
                     /locus_tag="L2X49_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system metallochaperone PcoC"
                     /protein_id="HBN4797423.1"
                     /translation="MSILNKAILTGGLVMGVAFSAMAHPELKSSVPQADSAVAAPEKI
                     QLNFSENLTVKFSGAKLTMTGMKGMSSHSPMPVAAKVAPGADPKSMVIIPREPLPAGT
                     YRVDWRAVSSDTHPITGNYTFTVK"
     gene            complement(6031..6921)
                     /gene="pcoB"
                     /locus_tag="L2X49_002344"
     CDS             complement(6031..6921)
                     /gene="pcoB"
                     /locus_tag="L2X49_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance outer membrane transporter
                     PcoB"
                     /protein_id="HBN4797424.1"
                     /translation="MKRNLKAIPVLVAGLFTSQLSIAAGSVSADPHAGHDMSAMQMPA
                     DENFTEMTSMEPIVTESRTPIPPVTDADRKAAFGNLQGHAIHDSAINYLVLLDQLEWQ
                     RSDNTNNFSWSVNSWIGGDTDRIWLKSEGERSNGETEAAEAQLLWGHAVGPWWDLVAG
                     VRQDFRPASARTWAAVGFQGLALYNFESEITGFVSNGGKAALRLGGEYDVLLTNRLIL
                     QPSYEVNFYSQDDESRGRGRGLTDTELGLRLRYEIRREFAPYIGVSWNQLYGKTSDMA
                     KREGEKDHQVVFLAGARIWF"
     gene            complement(6927..8744)
                     /gene="pcoA"
                     /locus_tag="L2X49_002345"
     CDS             complement(6927..8744)
                     /gene="pcoA"
                     /locus_tag="L2X49_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase PcoA"
                     /protein_id="HBN4797425.1"
                     /translation="MLLKTSRRTFLKGLTLSGVAGSLGVWSFNARSSLSLPVAASLQG
                     TQFDLTIGETAVNITGSERQAKTINGGLPGPVLRWKEGDTITLKVKNRLNEQTSIHWH
                     GIILPANMDGVPGLSFMGIEPDDTYVYTFKVKQNGTYWYHSHSGLQEQEGVYGAIIID
                     AGEPEPFTYDREHVVMLSDWTDENPHSLLKKLKKQSDYYNFNKPTVGSFFRDVNTRGL
                     SATIADRKMWAEMKMNPTDLADVSGYTYTYLMNGQAPLKNWTGLFRPGEKIRLRFING
                     SAMTYFDIRIPGLKMTVVAADGQYVNPVTVDEFRIAVAETYDVIVEPQGEAYTIFAQS
                     MDRTGYARGTLATREGLSAAVPPLDPRPLLTMEDMGMGGMGHDMAGMDHSQMGGMDNS
                     GEMMSMDGAGLPDSGTSSAPMDHSSMAGMDHSRMAGMPGMQSHPASETDNPLVDMQAM
                     SVSPKLNDPGIGLRNNGRKVLTYADLKSRFEDPDGREPGRTIELHLTGHMEKFAWSFN
                     GIKFSDAAPVLLKYGERLRITLINDTMMTHPIHLHGMWSDLEDENGNFMVRKHTIDVP
                     PGTKRSYRVTADALGRWAYHCHLLYHMEMGMFREVRVEE"
     gene            8979..9428
                     /locus_tag="L2X49_002346"
     CDS             8979..9428
                     /locus_tag="L2X49_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004388333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein"
                     /protein_id="HBN4797426.1"
                     /translation="MNILITTTAFTALFCGAAFAQSSDIAHEAHRFVNNASAVSHVNS
                     STHENLPDRVNKNNTPSFSEMNEHERAIVAHSFMNNSASYAHQKMIEEHKKMLSGSDA
                     NSKTSSSSFNELNAGEKAALVHEQVNNAGAEAHQTQARKLRGLYSTR"
     gene            9717..10454
                     /locus_tag="L2X49_002347"
     CDS             9717..10454
                     /locus_tag="L2X49_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBN4797427.1"
                     /translation="MYSTDVVKENAYLSATRSGLESNEIATLQRSLPSRFNLRHLKKN
                     ESLKLVLQKKAGKSRVVAYKFTSGSFNYTAYRISDKKFYNLSDTSGKGSLDYPLPATA
                     RLSSPFNPARLNPVSGKVSPHNGIDYSMPMNTKIVSVIDGKITRAEYNSTMGYFVEVT
                     GKAGVKTRYLHLNKILVTKGARVTRGGAIALSGNSGRSSGPHLHYELVINNNPVNSLA
                     FRAAAPADNKLEQHAFAHARDYERYLD"
     gene            complement(10488..10685)
                     /locus_tag="L2X49_002348"
     CDS             complement(10488..10685)
                     /locus_tag="L2X49_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HBN4797428.1"
                     /translation="MKSTTYALIAVAAIAAFALLREHWSHVAGYWPYLLLLVCPLMHL
                     FHGHGGHGDHQHHGSENDKKN"
     gene            complement(10726..13173)
                     /gene="silP"
                     /locus_tag="L2X49_002349"
     CDS             complement(10726..13173)
                     /gene="silP"
                     /locus_tag="L2X49_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag(+)-translocating P-type ATPase SilP"
                     /protein_id="HBN4797429.1"
                     /translation="MKNDNAVQHNNQTASEQTLSPDEGHVLHKVRDPVCGMAILPDRA
                     HSSIRYQDHQLYFCSASCESKFKAHPDRYLTEDASEHSHHHHHDHHEVSPDQIKQPHH
                     QAEKENSEGVWTCPMHPEIRRSGPGSCPVCGMALEPLVATASTGPSDELHDMTRRFWL
                     GLLLAFPVLVLEMGSHLFPELRNTVPPQYNTWLQLLLASPVVLWCGWPFFARAGMSLR
                     NRSLNMFTLVAMGTGVAWVYSVIATVFPSWFPASFRNMDGLVAVYFEAAAVITVLVLL
                     GQVLELRAREQTSGAITALLNLAPKTARRLDHDGHETDINAEDVLPGDKLRIRPGESI
                     PVDGIVIEGKTTVDESMVTGESMPVTKTEGDPVIGGTINQTGSLIIRAEKVGDETMLS
                     RIVQMVADAQRSRAPIQRMADSVSGWFVPLVILIAVVAFVIWSVWGPEPRMAHGLIAA
                     VSVLIIACPCALGLATPMSIMVGVGKGAQAGVLIRNAEALERLEKVDTLVVDKTGTLT
                     EGSPTVTGIISLNPGGETSLLRVTAAVEKGSQHPLGMAVVKAAQEKGIAIPAVTHFDA
                     PSGKGVSGDVEGQRVVIGNELAMQENSIVIDNQKAVADTLRMEGATVIYVATDGDLAG
                     LIAISDPVKTTTPDALKALRQAGIRIVMLTGDNQLTAEAVARKLGIDEVEAGILPDGK
                     KAVITRLKESGHVVAMAGDGVNDAPALAAADVGIAMGTGTDVAIESAGVTLLKGDLMI
                     LNRARHLSEITMKNIRQNLFFAFIYNALGVPVAAGLLYPVYGILLSPVIAAAAMALSS
                     VSVIVNALRLKSVRLGK"
     gene            complement(13300..13740)
                     /locus_tag="L2X49_002350"
     CDS             complement(13300..13740)
                     /locus_tag="L2X49_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797430.1"
                     /translation="MKKVVLMALALGLSLPAMASEKVIDMYKSDNCGCCSLWGKAMEK
                     DGFEVRTHVMNDQALSALKEKHAIPAGLRSCHTAVAGNLIIEGHVPATTIHKAMQSGS
                     GIYGLATPGMPAGSPGMEMGARKEAYDVIAFSPDGSKKVFQRIE"
     gene            complement(13827..16973)
                     /gene="silA"
                     /locus_tag="L2X49_002351"
     CDS             complement(13827..16973)
                     /gene="silA"
                     /locus_tag="L2X49_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit SilA"
                     /protein_id="HBN4797431.1"
                     /translation="MIEWIIRRSVANRFLVMMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     LYWARSRVLEYLNQVQGKLPAGVSSEIGPDATGVGWIFEYALVDRSGKHDLSELRSLQ
                     DWFLKFELKTIPNVAEVASVGGVVKQYQIQVNPVKLSQYGISLPEVKQALESSNQEAG
                     GSSVEMAEAEYMVRASGYLQSIDDFNNIVLKTGENGVPVYLRDVARVQTGPEMRRGIA
                     ELNGQGEVAGGVVILRSGKNARDVITAVRDKLETLKASLPEGVEIVTTYDRSQLIDRA
                     IDNLSSKLLEEFIVVAIVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWDHQHPGEQIDNATRWKVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYSMAGAAALAIIVIPILMGFWIRGK
                     IPAETSNPLNRVLIKAYHPLLLRVLHWPKTTLLVAALSIFTVIWPLSQVGGEFLPKIN
                     EGDLLYMPSTLPGVSPAEAAALLQTTDKLIKSVPEVASVFGKTGKAETATDSAPLEMV
                     ETTIQLKPEDQWRPGMTIDKIIEELDRTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLSDIDATAQSIEAVAKTVPGVVSALAERLEGGRYIDVDINREKASRYGMTV
                     GDVQLFISSAIGGATVGETVEGVARYPINIRYPQDYRNSPQALKQMPILTPMKQQITL
                     GDVADIKVVSGPTMLKTENARPASWIYIDARGRDMVSVVNDIKTAISQKVKLRPGTSV
                     SFSGQFELLEHANKKLKLMVPMTVMIIFILLYLAFRRVDEALLILMSLPFALVGGIWF
                     LYWQGFHMSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAHPELSRKETFTPEGLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLIWLRRHKKSVS"
     gene            complement(16984..18276)
                     /gene="silB"
                     /locus_tag="L2X49_002352"
     CDS             complement(16984..18276)
                     /gene="silB"
                     /locus_tag="L2X49_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit SilB"
                     /protein_id="HBN4797432.1"
                     /translation="MASLKIKYAAIIISSLIAGGLISVTAWQYVNSAQKTEKTEQKAP
                     ERKVLFWYDPMKPDTKFDKPGKSPFMDMDLVPKYADDSGDKSSGGIRIDPTQVQNLGL
                     KTQKVTRGMLNYSQTIPANVSYNEYQFVIVQARSDGFVEKVYPMTIGDHVKKGTPLID
                     ITIPDWVEAQSEFLLLSSTGGTSTQIKGVLERLRLAGMPEEDIQRLRSTRSIQTRFTI
                     KAPIDGVITAFDLRTGMNISKDKVVAQIQGMDPVWISAAVPESIAYLLKDTSQFEISV
                     PAYPDKTFHVEKWNILPSVDQTTRTLQVRLQVSNKDEFLKPGMNAYLKLNTRSQEMLL
                     IPSQAVIDTGKEQRVITVDDEGKFVPKQIHVLHESQQQSGIGSGLNEGDTVVVSGLFL
                     IDSEANITGALERMRHPEKTESSMPAMSDQPVNMHSGH"
     gene            complement(18390..18743)
                     /gene="cusF"
                     /locus_tag="L2X49_002353"
     CDS             complement(18390..18743)
                     /gene="cusF"
                     /locus_tag="L2X49_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux system protein CusF"
                     /protein_id="HBN4797433.1"
                     /translation="MRNSLKAVLFGAFSVMFSAGLHAETHQHGDMNTASDASVQQVIK
                     GTGVVKDIDMNTKKITISHEAIPAVGWPAMTMRFTFVNADDAINALKTGNHVDFSFIQ
                     QGNISLLKSINVTQS"
     gene            complement(18771..20156)
                     /gene="silC"
                     /locus_tag="L2X49_002354"
     CDS             complement(18771..20156)
                     /gene="silC"
                     /locus_tag="L2X49_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel SilC"
                     /protein_id="HBN4797434.1"
                     /translation="MFKLKLLSISTIFILAGCVSLAPEYQRPAAPVPQQFSLSHNSLT
                     PAVNGYQDTGWRNFFVDPQVTRLIGEALTNNRDLRMAALKVEEARAQFNVTDADRYPQ
                     LNASSGITYSGGLKGDKPTTQEYDARLELSYELDFFGKLKNMSDADRQNYFASEEARR
                     AVHILLVSNVSQSYFSQQLAYEQLRIARETLKNYQQSYAFVEQQLVTGSTNVLALEQA
                     RGQIESTRAEIAKREGDLAQANNALQLVLGTYRALPSEKGMKGGEIAPVKLPPNLSSQ
                     ILLQRPDIMEAEYQLKAADANIGAARAAFFPSITLTSGLSASSTELSSLFTSGSGMWN
                     FIPKIEIPIFNAGRNKANLKLAEIRQQQSVVNYEQKIQSAFKDVSDTLALRDSLSQQL
                     ESQQRYLDSLQITLQRARGLYASGAVSYIEVLDAERSLFATQQTILDLTYSRQVNEIN
                     LFTALGGGWVE"
     gene            20346..21026
                     /gene="silR"
                     /locus_tag="L2X49_002355"
     CDS             20346..21026
                     /gene="silR"
                     /locus_tag="L2X49_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver response regulator transcription
                     factor SilR"
                     /protein_id="HBN4797435.1"
                     /translation="MKILIVEDEIKTGEYLSKGLTEAGFVVDHADNGLTGYHLAMTAE
                     YDLVILDIMLPDVNGWDIIRMLRTAGKGMPVLLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGNTMITESQFKVADLSIDLVSRKVSRAGNRIVLTSKEFSLLE
                     FFIRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRAKIDNDYETKLIQTVRGVG
                     YMLEVPDA"
     gene            21019..22500
                     /gene="silS"
                     /locus_tag="L2X49_002356"
     CDS             21019..22500
                     /gene="silS"
                     /locus_tag="L2X49_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005763473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver sensor histidine kinase SilS"
                     /protein_id="HBN4797436.1"
                     /translation="MHSKPSRRPFSLALRLTFFISLSTILAFIAFTWFMLHSVENHFA
                     EQDVSDLQQISTTLNRILQSPVDPDDKKISKIKESIASYRNVALLLLNPRGEVLFSSA
                     QGAALRPAVNSADFSEHSRARDVFLWTVEDPAGPMDTGSKMKMETYRIIASSGQAIFQ
                     GKQQNYVMLTGLSINFHLHYLDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVS
                     NAIKNITSENLDARLEPTRVPIELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRT
                     PITNLVTQTEIALSQDRTQRELEDVLYSSLEEYNRMTKMVSDMLFLAQADNNQLIPDR
                     VMFDLRAEVMKVFEFFEAWAEERNITLKFNGMPCLVEGDPQMFRRAINNLLSNALRYT
                     PEGQAITVSIREQESFFDLVIENPGKPIPEEHLSRLFDRFYRVDPSRQRKGEGSGIGL
                     AIVKSIVEAHHGRVQVESDVHSTRFILSVPRLEKMIPDTQCWE"
     gene            22745..23176
                     /gene="silE"
                     /locus_tag="L2X49_002357"
     CDS             22745..23176
                     /gene="silE"
                     /locus_tag="L2X49_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="silver-binding protein SilE"
                     /protein_id="HBN4797437.1"
                     /translation="MKNIVLASLLGFGLISSAWATETVNIHERVNNAQAPAHQMQSAA
                     APVGIQGTAPRMAGMDQHEQAIIAHETMTNGSADAHQKMVESHQRMMGSQTVSPTGPS
                     KSLAAMNEHERAAVAHEFMNNGQSGPHQAMAEAHRRMLSAG"
     gene            23323..23673
                     /locus_tag="L2X49_002358"
     CDS             23323..23673
                     /locus_tag="L2X49_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBN4797438.1"
                     /translation="MKARNTLFAVLMLSLPAISAEHSEMKMTDMSTSASSQEYMAGMK
                     DMHDKMMAAVNESDPDKAFAKGMVAHHEGAIAMAETELKYGKDPKMRKLAQDIIKAQK
                     GEIEQMNKWLDSQK"
     gene            complement(23842..25668)
                     /locus_tag="L2X49_002359"
     CDS             complement(23842..25668)
                     /locus_tag="L2X49_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN4797439.1"
                     /translation="MRLRKLKLKNFRGYRNSTEIIIDESMTGIVGRNDFGKSTLLEAL
                     AIFFETEGMKADKNDMNCFSLREGDGRFEIACEFDDLPDFIMIDDRVQTTLASEHLLN
                     EDGNFEIVKTFKATTSGKPEQTCIRCIHPDEEPLRNLLGMKISELKAVGKEVEKNVAD
                     KRTASLWRQAIREAAAPYTCSEIMLDVDKEFGTDTKSLWGKILDLLPTYAIFKADRES
                     SDGDSEAKNPLQQAVKDAQAALQDKITALENEIQDSVLDVAQRTLDKLREMAPELASE
                     LTPRFKEKPKWTFNFTLDGENGIPINKRGSGIRRLILLNFFRAEAEKNVAGTPRNVIY
                     AIEEPETSQHPNYQMMLMKALLALAGQPHRQIIVTTHVPALAGLIPVEGVRYVTRNEA
                     GEPVVKMPDDAVLKEATESLGVLPETGMERAQGIVLVEGKSDVTFLRHAASSLKQSGA
                     LPASLEDVKIVPVLIGGCGSVKHWVTLNLAKDLGLPWCVFLDSDIGGDPAQVLSIQKR
                     KKEVEEAGKVFFATRKREIENYLCPDLIEEITGVAVTFTDTCDAKKIIGRAVGMKPDN
                     VLDKFWPQMTSERIISRSTYHDGTQERSELVEILSDIVSMTR"
BASE COUNT         6479 a         6700 c         6132 g         6685 t
ORIGIN      
        1 ttaggataaa tatttattta tcatggcctt ttaaccggta aaaaatggca ctcggcacag
       61 taaaccccgc tatagttatg ctatgcattt gtctttaatg atatctcgtc gttatattaa
      121 caggctgaaa ttatcattaa tgcacatttc attattagca gggatacctg aggagtttaa
      181 agtgaacata tatatcgggt ggcttttcaa attaatccct ttgattatgg gattaatttg
      241 catcgcgtta ggtggctttg tgctggaaag ttcagggcaa agcgagtatt tcgtcgcggg
      301 tcatgtgctg atttctctgg cggccatatg cctggcatta ttcactaccg catttattat
      361 catttcgcag ctcacgcgcg gcgttaatac gttttacaat acattgttcc ccattattgg
      421 ctatgcgggg tcaattatca ccatgatatg gggttgggca ctgttagcag gcaatgatgt
      481 gatggcagac gagtttgtcg ccggccatgt tattttcggc gttggtatga ttgccgcctg
      541 tgtatcgacg gtggcagcgt catccggtca ctttctgctc attcccaaaa atgcagcggg
      601 gagcaagagc gatggaacgc cggtacaggc ttattcttca ttaatcggta attgcctcat
      661 tgccgttccc gttttactca ccctgctcgg tttcatttgg tctattacgc tgttacgtag
      721 tgctgacata actccgcatt atgtcgcggg tcacgtattg cttgggttaa ccgcaatctg
      781 tgcctgtcta attggccttg ttgccacaat tgtccatcaa acacgtaata cgttttcaac
      841 taaagaacac tggctgtggt gttattgggt tatttttctc ggctcaatca cggtactgca
      901 ggggatatac gtcttagtca gttccgatgc aagcgcccga ctggctcccg gcattattct
      961 tatttgcctc ggaatgatct gttacagcat attctcaaaa gtctggctac tggcactggt
     1021 atggagacgt acctgttcgt tagccaacag aataccgatg attcccgtct tcacctgcct
     1081 gttttgcctt ttcctggcat cgtttcttgc ggaaatggcg cagaccgaca tgggatattt
     1141 tattccttcg cgagttctgg tcggtttggg agcggtatgc tttacgttgt tctcaatcgt
     1201 ttcaatatta gaagcgggtt ctgctaaaaa ataattgcaa cgtaccggat aaaaccagcg
     1261 ttgaccattt gcgtaacgct ggtttttctt aggcatcatg aaataacgca gcattaatgc
     1321 atagtggtta agtataaaaa acagcaaagt tactgttttt ttcaacctgt tcatatttca
     1381 taaagatctg gccactgggt gagttttcaa tcacctttcc atccacctta tattaagcat
     1441 ggagggtttc agttcacggg ctcattagaa aataatcaca agataaccac tatcaaaatt
     1501 agctcatttt taatgcgact ctaataattt tcatctttag gaaataggtc aggacgactt
     1561 tgctgcaatt gtgtagagac attaaagttg tatcctttaa cctgaaaaag actttaaagt
     1621 tgtaaagttt taaaaaaaca catacaaaac atgatgctaa gtgtgtatca cacgctgttc
     1681 caacattata ccttgatgtt cttgcaggaa ctgcaattta caggaggaaa aaggtgtttt
     1741 tgtagttatc tggcgttacg tgacagtgtt ggtcagatac ttccacctct tcagagttaa
     1801 agtttgatat atgggaaaaa aaacgctccc ggacgggagc gtaagccagt gacttcggcg
     1861 tcaaatgagg gtctaacaag tggagctgcg ggttaattct gggtgatctg actgcgatgc
     1921 ttatcagcct gctgctggtg aacaacggaa gactgaccat tattcgcctt ctcatgtaca
     1981 gctgcggcct tctcatgctc gctcatttca gaaaatgact ttgctgtttc gttggtagct
     2041 ccgggtttag ctttcatcat ttttttatgc atttcagcca tgtcctggtg ggcagcagaa
     2101 ttgctgtttt gcatcatgtc atgggacatt gcggcctgat cgtgactgct catatccatc
     2161 attgtcatgc tgtctccctg aattgcactt ttttcagcag atgactgcat ctggtgggca
     2221 ggtgcctgtg cattatttac ctggtcatgc atgttcattg tctctgcagc catggcagat
     2281 gaagcggcag ccataatggc aacaaatgat acgagaatct ttttcatggt tgggtctccg
     2341 gtgtttacat acccaaacaa tcaatggctt ataacagaga aagcagttga tctcagggcg
     2401 ggttaatcat cactccagga accaggtgat tgtgttatca cgctgctcct gatttatgaa
     2461 taattataaa aaaccgcctc tgtttatcgc gtgactgaat gatgacaatt ttgtcacctt
     2521 ccaggtttct cctgaacaac gggattaatc cattaacagg cgcactttga acacaatttc
     2581 acgcccctgc tgttctgctg acagctcgcc gccgtgagca tgaatgatcg accttgtaat
     2641 tgataatccc agccccgcgc cttccgtgtt gtagaacctt gatgagtctg cgcgatagaa
     2701 ccggtcaaac aaacgttcca gattagcggg aacctggccg gacatcgtat tcgtaatcat
     2761 cacgttcaca cagtcactgt cacgctcaag gtgtatcgct gtacaggtgt tatcgggaga
     2821 atacttgatt gcattggaaa gcaggttact gaaagcacgt cggagcatat cgctgtctcc
     2881 ggcaacaacg ccctctcctt caaccgtgat cgtctttcct gtttcgtctg ccaggggctc
     2941 gaacaactca cgtaattcat tcagttcggc tgccagatcc acatcatgtt tatccagccg
     3001 cagcagacca tgctctgaac gtgccagaaa aagcatgtca ctggtcattc gtgacaacct
     3061 tttcagttct tccaggttag cgaataaaat ttcgcggtaa tgcgaaacat ccctttcctt
     3121 agccagtgca aactgcgtct gcatcatcag attactgact ggtgtgcgca gctcatgcgc
     3181 gatgtcagac gagaaatctg acagtttccg gaatgccccc tccaggcgat caaacatatt
     3241 attgaactcc tgcatggtct cagagatttc cggcggagcc agatcgggat ttagacgctg
     3301 atccaggctg tgtacggtca tggaggaagc cagactggtc atttcccgta gcggtttcag
     3361 accaatacgt gtggtcagcc agcccagaaa aacagaaata aagaccagac cgatattgaa
     3421 ccagaacagc caggtactga gtttgtccat aaacagggtg tgatacccag tatccgtggc
     3481 aaccgtaatg atgacatgtt tgcttttacc ctgttccggc gtcacggcaa cccgccgcga
     3541 gatactgcgg tacacggtgt tattttcttc cgtctggatc atatagtcga gaatatcacc
     3601 cgacttatta agcaggaccg ctggaacaac agaatttttg gcatagagtt caacaatttt
     3661 ttcattttcc atgtttttta tagaaatgaa taagccattg tgccccacca tcgcatcgtt
     3721 tattttttct gataatgact taatatccgt tttgttcctg aacgtctctg ttttaagaaa
     3781 ctcttcggtg agctgaagtt tacctgtcag aaaatcgcgg tcctgattat cgaaatagcc
     3841 attaagggtg ctaatcagga taaaacttga taaccaccat accgtaagca tcaccgcaga
     3901 aaaaatcagg ctcaggcgtg tggtcaggga aattttgaac ctcactcttc tctgatctcc
     3961 aggacatatc cggcaccgcg aacggtatgg atcagttttg gctcaaagtc atcatcaatt
     4021 ttacttctca gacgtctcac ggcgacatca atcacattcg tatcactgtc aaaattcatg
     4081 ttccagacca gggacgagat aagactcctg ggtaacactt ctccggtgcg ttgcagcagc
     4141 aactcaagca gaacgtattc tttaccggtg agatggatct tcttccccga acggatcacg
     4201 gtccggcgca ccatatcaac ggtcatatcg gcgatggtgc agactgttgc ggcctgcgag
     4261 cgtgcccggc gcagtagggt tcttacacgt gcaaccagct ccgtaaaatc aaagggctta
     4321 atcaggtagt catctgcgcc aagctccagt cctttcactt tgtcccgcac gttgtccttt
     4381 gcggttaaaa acaggaccgg ttcttcgtgc ccggactccc tcagtgcgct gatgatttgc
     4441 cacccgtcga ggaaaggcag catcacgtcc agtattatca aatcatactg tcccttcgac
     4501 gcggccccga gaccatcgcg gccattatta aagagatcgg cctgatagcc ttcctcaacc
     4561 agtccctgct gcaggtaacg acctgttttt tgttcgtctt caacgattaa aatacgctgc
     4621 atggtcaact cgctgatatg aaagtaaaaa tctcacgcat gagctttggc tgtcccctag
     4681 ctgacctgag acggagcaag cattccgagc cacgctacgg cgcccagaat gataatcgca
     4741 acaacgaatt ctgtcaggat gctgtttcgc atcagggcaa cgctgcgatc ataattccct
     4801 tccctgacca taacttcaag ccggggaccc aggtgaaacc ggtttgctgc agccagaaga
     4861 agcatcagaa caaacagagc cgtcttggca agcaatatcc tcccccagga actgttgaat
     4921 aagggagtta agttaccctc agcaatatac agatagttga ccagcgcact caggatcagg
     4981 gctacaacaa tcaccgttcc tgccgtggca aattttgcca gggagtcaga tatcacaatg
     5041 acgctctgtg cattatgctc gtttctgcgc atcagcagga tagcaaatgc aaccagagca
     5101 cctgtccagg cacctgcagc gccgagatgg gtcagatcgc tcagtaaatg gagatagtaa
     5161 tgcagaccgt catgcataac ggcgtgtcct ccccaggcaa gtgtagccag cgccacgccc
     5221 ccactcatcg tcatcagcag gcaggacaat actctcttat tagtgtaaag gaacaaagca
     5281 ccgagtgtgg taaacagggc acagagcctg acaatccagc taatacccac atcagtttct
     5341 tctatcacca tctcgataac atggatggat aattctctga ggtcagttac tccactcatg
     5401 gcattagata ccaggagcat attaatgcca gtaagaatga tgcctgtaac aacagcaaaa
     5461 gttataaacg acctgaaatt agtcaggtta taggtttcat gtctgacacc gcttattcca
     5521 tatatctgaa aaaatggcaa tccaaatatt accatcaaat ccagataaag aagaaaacga
     5581 ataacaatca taatcaggtc gttcataata ttacttcact gtaaaggtgt aattaccggt
     5641 aatagggtgc gtatctgaag aaaccgcgcg ccagtcaaca cgataagtgc cagcgggtaa
     5701 aggctctcgc ggaataatga ccatcgattt agggtcagcg cctggcgcca cttttgccgc
     5761 gaccggcatc ggagaatgtg atgacatgcc tttcataccc gtcatcgtta attttgcacc
     5821 tgagaatttc acggtcagat tttccgagaa attaagctga atcttttccg gggccgctac
     5881 ggctgaatca gcctgtggca cagagctttt taattccgga tgggccatag cagaaaaagc
     5941 aacgcccata acgaggccac ctgtaagaat ggctttattt aaaatcgaca ttttatttac
     6001 ctgtttagtt gagtgtttta tatcagtgcg ttaaaaccag attctggctc ccgccaggaa
     6061 tactacctga tggtctttct caccttctct tttcgccata tcggatgttt tcccgtaaag
     6121 ttgattccag gaaacgccta tatagggtgc aaactcacgg cgtatttcat agcgcagccg
     6181 gagccccagc tctgtgtcag tcagtcccct gccgcgaccc cgcgattcat catcctgact
     6241 gtagaaattc acctcatagg atggctggag tatgagccgg ttagtcagta aaacgtcgta
     6301 ttctcctccc agacgaaggg ctgcttttcc gccattactg acaaaacccg taatttcaga
     6361 ctcaaaatta tagagtgcca gcccctgaaa accgacagca gcccaggtcc gggcagaagc
     6421 aggtctgaaa tcctgcctga cacccgcaac caaatcccac catgggccaa ccgcatgtcc
     6481 ccagagtaac tgcgcttcag ccgcctccgt ttccccattg cttcgttcac cttcactctt
     6541 tagccaaatc cgatctgtgt cgcctccaat ccagctgtta acactccagc tgaaattgtt
     6601 ggtgttatcc gaccgttgcc attccagttg atccagcaga accagataat taatcgcact
     6661 gtcgtgaatc gcatgcccct gtaaattgcc gaatgcagcc ttccggtcgg catcggtaac
     6721 aggcggaatt ggcgttctgc tctcagttac aatgggctcc attgacgtca tctcagtgaa
     6781 attctcatct gctggcatct gcatggcaga catgtcgtgc ccggcgtggg gatctgcaga
     6841 gacggagccc gccgcaatag aaagctgtga ggtaaacaaa ccggcgacca gaacaggtat
     6901 ggccttcaaa tttctcttca ttcgcatcat tcctccaccc ggacttcacg aaacattccc
     6961 atttccatgt gatagagcaa atggcagtga tacgcccagc ggccaagcgc atctgctgtc
     7021 actctgtaac tgcgttttgt accaggggga acatctattg tgtgtttacg aaccatgaaa
     7081 ttaccgtttt catcttccag atcgctccac ataccatgca ggtgaatggg gtgagtcatc
     7141 atggtatcgt tgatcagcgt gatcctgagc cgctcaccgt atttcagcag caccggtgcg
     7201 gcatctgaaa acttgattcc gttaaatgac caggcaaact tttccatgtg gccggttaaa
     7261 tgcagttcta tggtacggcc aggttcacgt ccgtcaggat cctcaaagcg gcttttcaaa
     7321 tccgcgtacg tgagaacctt tcttccgtta tttcgaagac caatacccgg atcatttaat
     7381 ttcggagaga cgctcatcgc ctgcatatca accagtgggt tatccgtttc tgacgcagga
     7441 tgactttgca tacccggcat tccggccatc cgggaatgat ccataccggc catgctgctg
     7501 tgatccatgg gcgcggagga tgtcccgcta tccggaaggc cagcaccgtc catagacatc
     7561 atctctccgc tgttatccat gcctcccatc tggctgtggt ccattcctgc catatcatgt
     7621 cccattcccc ccatacccat atcttccatg gtcaacagag gacggggatc gaggggggga
     7681 acggcagcac ttaacccctc tctcgtggcc agtgtccctc gagcgtaacc ggtcctgtcc
     7741 atggattgtg cgaagatggt ataggcctca ccctgaggct ccacaatgac atcataggtt
     7801 tcggcaacgg caatcctgaa ttcgtcaacg gtaaccgggt ttacatactg gccatctgca
     7861 gccacgaccg tcattttcag cccggggata cggatatcga aataggtcat tgccgagccg
     7921 ttgataaacc gtaagcgtat cttttcaccg ggacggaaca gtccggtcca gtttttcagc
     7981 ggggcctgcc cgttcatgag ataggtgtag gtgtagccac tgacatccgc gaggtcagtc
     8041 ggattcattt tcatttcagc ccacattttc cgatcggcaa tggtggctga cagccccctg
     8101 gtattcacgt cgcggaaaaa agagccaacg gttggtttat tgaaattgta gtaatccgac
     8161 tgttttttta attttttcag caggctgtga ggattttcat cggtccagtc agacaacatg
     8221 accacatgct cacgatcgta agtaaacggt tctggctccc cggcatcgat gataatggca
     8281 ccgtataccc cctcctgttc ctgcagaccg gaatggctgt ggtaccagta agtcccgttc
     8341 tgcttaacct taaaggtgta aacgtaggta tcatcaggct ctatgcccat aaaactcagc
     8401 cccggaacac catccatatt ggccggaaga ataatgccgt gccagtgaat ggacgtctgt
     8461 tcattaagac ggtttttgac cttcagggta atggtgtcac cttctttcca gcgaagaacg
     8521 ggccccggca ggcctccatt gattgttttg gcctgacgct cactgcccgt gatattgacg
     8581 gccgtttcac caatggtcag gtcaaactga gtaccctgca gggatgcggc aactggcagg
     8641 ctcagactgg aacgcgcatt gaaactccat acgccaagac ttccggctac gccagagagg
     8701 gttaacccct tcaggaaagt tcgtcgagac gttttcaaca gcatgcgcat tcccttattt
     8761 aaagtatggt tactgacaga attcgagaac cgatttaaat taaattactg gttcatccac
     8821 ttacaatgaa atgaatctag cacaccttaa gaaacaaatt cattacaatc gtgtaatgta
     8881 catagctgta ttacatttac gtcatcttcc ccacaggttg attattttta tatatgatca
     8941 taaggacatc ttttatgtac ctcagaaggt aattacacat gaatatatta atcacgacca
     9001 ctgcgtttac agctttattt tgtggggcag cttttgctca gtccagtgat attgcccatg
     9061 aagcacatcg atttgttaat aatgcctcag ccgtcagtca tgtgaattcc tcgacgcatg
     9121 aaaacttacc ggacagggtt aataaaaaca acacgccctc attctctgaa atgaatgaac
     9181 atgaaagggc cattgttgct cattcattta tgaacaacag cgcgtcctat gcgcatcaga
     9241 aaatgattga ggaacataaa aaaatgctgt ccggcagtga tgcaaattca aagacctcgt
     9301 cttcttcttt taacgaactg aatgccggag aaaaagccgc tctcgtgcat gagcaggtca
     9361 ataatgccgg tgcggaagca catcagacgc aggcaagaaa gcttcgcggg ctgtattcga
     9421 ccaggtaact gcaggcgggt tagtgcttag cgtcaggtgc gcagcagggc tttactgacg
     9481 ggttacgtag caggacatga gccccatatt gctctgccgt aacctgtttc agtcccgtta
     9541 atcatcaccg tcgggctggt catacagttc ccagagcttc aggagcaaac gggaaacaag
     9601 atatgttaca aaaatgacga ccatcaacgg tgctcccgat taactgacag atgacacgcc
     9661 tcctgaaaac ccctagcata cgccgcagtg ttcatgctaa cggcgaattc cagtaaatgt
     9721 attctaccga tgtcgtaaaa gaaaatgcct acctttcagc cacccgctcg gggctggaat
     9781 cgaacgagat cgctactctt cagcgctcct tgccttcccg gtttaatctg cggcatttga
     9841 aaaaaaatga atcattaaaa ctcgtactgc aaaagaaagc gggaaaatca cgtgtcgtgg
     9901 cctataaatt tacgtccggt tcatttaatt acacggcgta tcgtatatca gataaaaagt
     9961 tctataacct ttccgatact tccgggaaag gcagtctcga ttatccgtta ccggccacag
    10021 caagactcag ttcgcctttc aatcctgcaa gacttaaccc ggtatcggga aaagtgagtc
    10081 cccataatgg cattgattat tccatgccca tgaacacgaa aatagtcagc gtcatcgacg
    10141 gaaaaatcac ccgggccgaa tacaacagta ccatgggata ttttgttgaa gtaacgggaa
    10201 aagccggtgt taaaactcgc tatctccacc tcaataaaat actcgttact aaaggggcca
    10261 gggttacacg gggaggtgct attgcgttat ccggtaacag cggacgttca tccggtcctc
    10321 atctgcatta cgagctggtc atcaataaca accctgttaa ctctctggcg ttccgggcag
    10381 cggcacccgc tgataacaaa ctcgaacagc atgcctttgc gcatgccaga gactacgaac
    10441 gatacctgga ctgataacgg gaccgcgacg cggccccgtc tgccggatta atttttttta
    10501 tcgttttcac ttccgtgatg ttgatgatct ccatgccctc cgtggccgtg gaaaagatgc
    10561 attagcgggc agaccagcaa taacagatat ggccagtaac ctgccacatg tgaccagtgt
    10621 tcgcgcagga gggcaaatgc cgcgatcgcg gcgacagcaa taagcgcata ggtggtactt
    10681 ttcatactgg actccttctg ttcgtaactg acccttcact cagtgttatt tcccgagcct
    10741 gacacttttc agacgcaacg cattcacaat gacgctgacg gaggaaagag ccatggccgc
    10801 cgccgcaata actggcgaca gcagtattcc atacacagga taaagcagac ctgcagccac
    10861 aggcacgcca agtgcgttgt agataaatgc aaaaaacaga ttctgacgga tatttttcat
    10921 ggtgatctct gacagatgac gggccctgtt cagtatcatc aagtcgcctt tgagaagggt
    10981 gactccggca ctttcaattg ccacatctgt acccgttccc atggctatac ccacgtcagc
    11041 cgctgccagc gccggggcat cattcacacc gtctccggcc atcgcaacca catggccaga
    11101 ctctttcagt cgggttatca ctgctttttt gccatccggc agaattccgg cttcaacctc
    11161 atctattccc agtttccgtg cgactgcttc agcagtaagc tggttatccc cggtgagcat
    11221 aacgatgcgg atccccgcct gacgcaaagc tttaagcgca tccggcgtgg ttgttttcac
    11281 gggatccgag atagctatca ggcctgcaag gtccccgtct gtggccacat agataacggt
    11341 agcgccttcc atccgcaacg tatccgcaac ggccttttga ttatcaataa ctatactgtt
    11401 ttcctgcata gccagttcat taccaataac aacccgttga ccttcgacat cgcctgagac
    11461 acctttaccc gacggtgcat cgaaatgagt gactgcgggt attgcgatcc ccttttcctg
    11521 tgctgcttta actactgcca tacccagcgg atgctgcgag cctttttcca ctgcggctgt
    11581 tacacgcaaa agagatgttt ccccacccgg attgagactg ataatccctg tcaccgtagg
    11641 cgaaccttcc gtgagcgtgc ctgttttgtc gacaaccagc gtgtccactt tttcaagacg
    11701 ctcaagggct tcggcattcc tgattaacac cccggcctgg gctcctttgc ccacccccac
    11761 cattatcgac atcggcgtgg ccagccccag cgcgcaggga caggcaataa tcaggaccga
    11821 cacagccgca atgagaccgt gcgccatcct gggctcgggc ccccagacag accagatcac
    11881 gaaagcaaca accgcgataa gtatcaccag aggaacaaac cagcctgaaa cgctgtcagc
    11941 cattctctgg atgggggcac gcgaacgctg tgcatcagcg accatctgaa caattcgtga
    12001 gagcattgtt tcatcaccga ctttctctgc acggatgatg agactccctg tctgattaat
    12061 ggtccccccg atgacagggt caccctccgt tttggtaacc ggcatagatt ccccggtcac
    12121 catcgattca tcaacggttg ttttgccttc gatcacgata ccgtcgaccg gaatactctc
    12181 tccaggtctg atgcggagct tatcgccagg caggacatct tccgcattaa tatccgtttc
    12241 atgaccgtca tgatccagcc gtctggcggt tttgggggca aggttcagaa gcgcagtaat
    12301 ggcgcctgag gtttgttccc gtgcccgcag ctcaagaacc tgtcccagca gaacaagcac
    12361 cgtaataact gctgcggctt caaaataaac ggccaccagg ccatccatgt ttctgaacga
    12421 tgcaggaaac caggagggga agacggttgc aatgacgctg taaacccagg ctacgccggt
    12481 ccccattgca acaagggtaa acatattcag ggagcggtta cgtaacgaca ttccggcccg
    12541 ggcgaagaat ggccagccac accacaacac gacaggggag gccagaagca gctgcagcca
    12601 tgtgttgtac tgtggcggta ctgtattcct caactcggga aacagatgag atcccatttc
    12661 gagtaccaga accggaaacg ccagcaacaa ccccagccag aagcgtcttg tcatgtcgtg
    12721 aagttcatca ctcggccccg tggatgccgt agctacgagc ggctccagtg ccattccaca
    12781 gacaggacag cttccgggac cactgcggcg tatctccggg tgcatcggac atgtccacac
    12841 accttcagaa ttctcttttt ccgcctggtg gtgaggctgt tttatctgat cagggctgac
    12901 ttcgtgatga tcgtggtgat ggtgatggga atgttcactg gcatcttcgg taagataacg
    12961 atcgggatgg gctttaaatt tactctcaca gctggcggag cagaaataaa gttgatggtc
    13021 ctggtatcga atgctgctgt gcgccctgtc gggcaggatg gccatcccgc acacgggatc
    13081 tctcacctta tgcaatacgt ggccctcgtc cggggataat gtctgctcag aagcagtctg
    13141 gttgttgtgt tgcactgcat tgtcattttt cacagtaact ctccttatgc ataccgaagc
    13201 atcttctgtc ttcagggtat gtcatggatg cgattaccac gaatatcgcc ggcacaggag
    13261 tgcctgctac cggcgtcccg ttatcagccg ttccgctgac tattcgattc gctggaagac
    13321 ttttttactt ccatccggtg agaatgcgat aacatcgtaa gcctcttttc gggctcccat
    13381 ctccattcca ggacttcctg ctggcatacc gggggtggcg agaccgtata tacctgaacc
    13441 agactgcatt gccttatgta tcgttgtcgc aggcacatgg ccttcaatga tcaaattacc
    13501 ggcaaccgcg gtatgacaac ttcgcagtcc tgcaggaata gcatgctttt ctttcagggc
    13561 tgacagcgcc tgatcattca tgacgtgagt tcgtacttca aacccgtctt tttccatcgc
    13621 cttgccccac agggaacaac agccacagtt gtcagatttg tacatatcaa tcactttttc
    13681 actcgccatc gcgggcagtg aaaggccgag agccagcgcc atcagaacca cttttttcat
    13741 actcacctct gtatattatc gatttaaacc ctgacgtcag ggtgaatcag taaagaagga
    13801 cacccacagg gggtgccctt tcaggttcag gacacgcttt ttttatgtct gcgcagccag
    13861 attaatttgt aggcggcagg aataatgaac agggacagca gcggagccgt gatcatccca
    13921 ccaatcatgg gtgccgcgat acggctcatg acttctgaac ctgcaccggt tccccagagt
    13981 attggcagca gacccgcaat gatcaccgcc acggtcatgg ctttcggccg gacacgcagt
    14041 acggcaccat gatagagggc ttcatcaagg ccttccggtg tgaacgtctc tttacgggac
    14101 aattccgggt gcgcttcaat ggcatgacgc agatacatca gcatgaccac gccaaactct
    14161 gctgccaccc cggccagagc gataaacccc gttccggttg cgacagacat atggaagccc
    14221 tgccagtaca ggaaccatat cccgccaacc agggcgaacg gcaggctcat caggatcagc
    14281 agggcttcat caacccggcg gaatgccaga tacaacagga tgaagatgat catcaccgtc
    14341 atcggcacca tcagcttcag tttcttgttg gcatgctcaa gcagttcaaa ctgtccggag
    14401 aatgacacgc tggtacccgg tctcagtttc actttctgac tgatcgccgt tttaatgtca
    14461 ttaaccaccg acaccatatc cctgccgcgc gcgtcaatgt aaatccagct ggctggacgg
    14521 gcattttccg ttttcagcat agtcggcccg gaaacgacct taatatccgc aacatcgccc
    14581 agcgtgatct gctgcttcat cggggtcagg atcggcatct gtttcagggc ctgcgggctg
    14641 ttccggtaat cctgcggata gcggatatta atcgggtacc gggccacgcc ttcaaccgtt
    14701 tcccctaccg tcgcgccgcc gatggctgat gagatgaaca gctgcacatc gcccaccgtc
    14761 attccgtagc gggaggcttt ttcccggttg atatccacat caatgtagcg cccgccttcc
    14821 agtcgctctg cgagagcaga cactacgccg ggtacggttt tggcgaccgc ttcgatactc
    14881 tgcgccgttg catcgatatc ggacagaacc gttccggaca ccttgatgcc tatcgggctt
    14941 ttgatcccgg ttgagagcat atcaatacgg ttacggattg gcggcaccca gaggtttgcc
    15001 agccccggta aacggacggt cctgtcgagt tcttcaataa tcttgtcaat cgtcatgcct
    15061 ggacgccact gatcctcagg tttgagctgg atcgtggttt caaccatttc gagcggtgcg
    15121 gaatccgtgg cggtctctgc tttaccggtc ttgccaaata cagaggccac ttcaggaacg
    15181 cttttgatta acttgtccgt cgtctgcagg agcgctgcag cttcagccgg agagacaccc
    15241 ggcagggtcg acggcatata caacaggtcg ccctcgttaa tcttcggcag aaactcaccg
    15301 cccacctggc tcagcggcca gataacggtg aaaatggaca aggccgcaac cagcagggtt
    15361 gtttttggcc agtggagaac ccgcaacagc aatggatgat acgctttgat cagtacccgg
    15421 ttcagggggt tactggtctc tgcaggaatt ttcccccgga tccagaatcc catcagaatc
    15481 ggaatgacga tgatggccag cgcggccgcg cccgccatgg agtacgtttt cgtgaatgcc
    15541 agcgggccaa acagacgtcc ttcctgacct tccagggtaa agataggaat aaaggacagg
    15601 gtgatgatca gcaggctgat aaacagtgcg ggtcccactt caacggaggc atcggtaatc
    15661 accttccagc gggtggcgtt gtcaatctgc tcacccggat gctgatgatc ccactcctca
    15721 agccgcttat gcgcattctc aatcatcaca atggcggcat ccaccatcgc accgacggca
    15781 atcgctatcc ctcccagcga catgatattg gcgttcagtc cctggaagtg catgacgata
    15841 aaggcgatac acagaccaag cggcagagag ataatcgcca ccagggcaga acgtacgtgc
    15901 cacaggaaca gggcacagac gatggccacc acgataaact cttccagaag tttggaactg
    15961 aggttatcaa tcgcccggtc gatgagctgg ctgcgatcgt aggtggtcac gatttcaacg
    16021 ccttccggca ggctggcctt cagcgtctca agtttatccc tcactgccgt gataacgtcg
    16081 cgtgcatttt tacccgaccg caggatcacc acgccgccag cgacttcgcc ctggccgttc
    16141 agctcggcaa taccacgccg catttcaggc ccggtctgca cgcgggcaac atcccgcaga
    16201 taaaccggca cgccgttctc gcctgttttc agaacgatgt tattaaaatc atcaatgctc
    16261 tgaagataac cgctggcacg gaccatatac tcagcttcgg ccatttcaac ggatgagcca
    16321 ccggcctcct ggttagacga ttcaagggcc tgtttcactt cgggcaggct gataccatac
    16381 tgagacaatt ttaccggatt gacctgaatc tggtactgtt tcaccacgcc gccaaccgaa
    16441 gcgacctcag ccacgttcgg gatggttttc agctcaaatt tcaggaacca gtcctgcaga
    16501 gaacgcagtt ctgaaaggtc gtgttttccg ctgcgatcga caagggcata ttcaaatatc
    16561 cagcccaccc ccgtggcgtc cggaccgatt tcagaactca caccggcggg cagttttccc
    16621 tgtacctggt tcaggtactc cagcacgcgg gaacgggccc agtacagatc ggtgccgtct
    16681 tcaaaaatga catacacata cgaatcaccg aactgtgaaa aaccacgcac ggtttttgcg
    16741 ccaggcacgg acagcatggt ggtggtaagt ggataggtga cctggttttc tacaatctgc
    16801 ggggcctggc cgggatagct ggttttgata atgacctgca catctgacag gtcaggcaag
    16861 gcatcgacag gcgtgttaat aatcgtccat gtgccccaga tgctgagaaa cagggccccc
    16921 atcatgacca ggaaacggtt ggcgacagag cgccggataa tccattcaat catcgtcgtc
    16981 tcctcagtgc cctgaatgca tatttacagg ctggtcagac attgctggca tactgctttc
    17041 tgttttttca gggtggcgca tacgttccag tgcgcccgta atattggctt cagagtcaat
    17101 gaggaacagg ccactgacca ccacggtatc gccttcattc aggccggagc cgatgccgga
    17161 ctgttgctgt gactcatgca gaacgtggat ctgtttcggc acaaacttgc cttcatcatc
    17221 aacagtaatc acgcgctgtt ctttgccggt atcgataacg gcctggcttg gtatcagcag
    17281 catctcctgg cttctggtat tcagtttcag ataggcattc atgcccggct tgagaaactc
    17341 atccttatta gaaacctgga gacggacctg aagcgtacgg gttgtctgat ccacgctggg
    17401 aagaatattc catttttcga catggaatgt tttatccgga taagccggta ccgaaatttc
    17461 aaactgcgac gtatctttca gcagataggc gatagattct ggcactgcag cgctgatcca
    17521 gaccgggtcc attccctgaa tctgagccac caccttatct ttcgaaatat tcatgccggt
    17581 gcgcaggtca aatgcagtaa tgacaccatc aataggtgct ttaatggtaa aacgggtctg
    17641 aatgctccgg gtagaacgca gtctctgaat atcctcttcc ggcatacctg ccaggcgaag
    17701 ccgttccaga acgcctttaa tttgcgtgga agtaccaccg gtgctggata acagaaggaa
    17761 ttcactctgt gcttccaccc agtccggaat ggtaatatcg ataagtggcg tccccttctt
    17821 cacatgatcg ccaatcgtca tggggtagac tttttcgaca aacccgtcag aacgcgcctg
    17881 cacaataaca aactgatact cgttatagct gacattagcc gggattgtct gagaataatt
    17941 cagcattcct cgcgtgactt tttgcgtttt taatcccaga ttctgaacct gggttgggtc
    18001 gatacggatc ccgccactgc ttttatcgcc actgtcatca gcatattttg gcaccaggtc
    18061 catatccata aagggcgatt ttccgggttt atcaaatttg gtatccggtt tcattgggtc
    18121 ataccagaaa agcacctttc gttccggtgc cttttgttct gttttttctg tcttttgtgc
    18181 tgagtttaca tactgccagg cagtaaccga tatcagcccc cctgctatga ggctgctgat
    18241 aattattgca gcatatttta tctttaaaga agccatacaa ttttccgcta aaaaatcaaa
    18301 acacctggca tatgcgcctg acggttaact cacacatata cctatgtgaa tgtacaggcg
    18361 cacaggatgt aatcgctccg gacagccaat caggactgcg tcacgttaat gcttttgagt
    18421 aaggagatat tgccctgctg aataaacgag aaatcgacat ggttgcctgt tttcagggca
    18481 ttaatagcat cgtctgcatt aacaaaagta aagcgcatgg tcattgcagg ccagcccacc
    18541 gctggaattg cttcatgcga aatggtgatt ttcttagtat tcatatcaat gtctttaacg
    18601 acaccggtgc ccttgataac ttgctgtacc gaagcatcac tggcagtatt catatcgcca
    18661 tgctgatgtg tttcagcatg aagaccggca gaaaacatga cagagaaggc accaaataaa
    18721 acggctttaa gtgaattacg cattttaatt tcctgattaa ttaaataaat ttactctacc
    18781 caaccgccac ccagcgcggt aaacagatta atttcgttaa cctgccggga ataggtaaga
    18841 tcgagaatgg tttgctgggt agcgaagagg gaacgttctg catccagcac ttcgatgtaa
    18901 ctgacagcac cacttgcata caatcctctg gcacgctgga gagttatctg aagtgaatca
    18961 agataacgct gctgtgactc aagttgctgg ctaaggctgt cgcgcagcgc aagcgtgtcg
    19021 gaaacatcct taaaggctga ctgaattttt tgttcgtaat taaccaccga ctgttgctgg
    19081 cgaatttcag ccagcttcag attggcttta ttcctgccag cattaaatat aggaatttca
    19141 attttaggga taaaattcca cattccactt cctgacgtaa acaggctgga cagctccgta
    19201 ctgcttgcgg aaagaccact ggtcagggta attgagggga aaaaggccgc tcgcgctgcg
    19261 ccaatattgg catcagccgc tttcagctga tattccgctt ccataatatc cggtcgctgc
    19321 agcaaaattt gtgaagacag atttggtggc aattttactg gggctatctc cccgcctttc
    19381 atcccttttt ctgacggaag tgcgcggtac gttcccagca ccagttgcag ggcattgttt
    19441 gcctgagcca gatcgccttc tcgtttggct atttcggcgc gggtactttc gatttgtcct
    19501 cgcgcctgtt caagtgccag aacgttcgta ctcccggtca cgagctgctg ctcaacgaaa
    19561 gcataggact gttgataatt tttcagcgtt tcccgcgcaa tacgaagttg ttcgtacgcc
    19621 agttgctggc tgaaatagct ttgtgaaacg ttggatacca gcaggatgtg tacggcccga
    19681 cgggcttctt cgctggcaaa gtagttctgg cgatcagcat cactcatgtt cttaagtttg
    19741 ccgaaaaaat cgagctcata gctgagctcc agtctcgcgt cgtactcctg tgtggtcggc
    19801 ttgtcacctt tcagaccacc gctgtatgtt atcccggatg aggcattcag ctggggataa
    19861 cgatctgcat ccgtgacgtt gaactgggct cgggcctctt caaccttcag ggcagccatt
    19921 ctcaaatcac ggttattagt cagagcttca ccgatcaacc gggtaacctg gggatcgaca
    19981 aaaaagttac gccagcccgt atcctgatag ccatttaccg ctggcgtcag gctgttatgg
    20041 gacagtgaaa actgctgggg taccggtgct gcgggccgct gatattcagg cgcaagcgac
    20101 acgcagcctg ccaggatgaa tatcgtgcta atgctgagta attttaattt gaacataacg
    20161 cttctcgtcc aggcagacct ggtaaatggt tcaaatgaag tccagagtat tgataggggt
    20221 actttaccta acgctctctg tttgccgggt gacaggataa tgacaatgtt gtcatttttg
    20281 ctgtaatccg ttgataacga tcgtgggcgc aatagaatga cattagcagc cagccgggga
    20341 gcaagatgaa aatattgatc gtcgaagacg aaattaaaac aggtgaatat ctcagcaaag
    20401 ggcttacaga ggcagggttc gtagtggatc acgctgataa tggtcttacc ggatatcatc
    20461 tcgccatgac agccgagtat gatttagtca ttctggatat catgctacct gatgtgaacg
    20521 gctgggatat catccgcatg ctgcgcactg ccggaaaggg tatgccggtc ttactgctga
    20581 cagccctcgg cacgatcgaa catagggtca aaggactgga actgggtgcg gacgattatc
    20641 tggttaaacc ctttgcgttt gccgaactgc tcgcccgggt gagaaccctt ctgaggcggg
    20701 gaaacacgat gatcacggaa agccagttta aggtggctga cctctcgatt gatctcgtat
    20761 ccagaaaagt cagtcgcgcc ggaaaccgca ttgtgctcac cagtaaagag ttcagcctgc
    20821 tggaattctt cattcgccat cagggagagg ttcttccccg ctccctgatt gcctctcagg
    20881 tctgggacat gaattttgac agcgacacta atgcgatcga tgtcgcagta aagcgactcc
    20941 gcgctaaaat agacaacgat tacgagacaa agctgatcca gacagtccgg ggcgtgggct
    21001 acatgctgga ggtcccggat gcatagcaaa ccttccagac gccctttctc actcgctctg
    21061 cggctgacct tttttatcag cctgtccacg atactggctt ttatcgcatt cacctggttt
    21121 atgctgcatt ctgttgaaaa tcattttgcc gagcaggatg tcagcgatct tcaacaaatc
    21181 agcaccacac tgaaccgtat actgcagtcc ccggtggatc cggatgataa aaaaataagc
    21241 aaaataaagg aatcaattgc cagctaccgc aacgttgccc ttttgctcct caatcccagg
    21301 ggggaagtgc tctttagctc agctcagggg gcggcactac gcccggcagt gaattcagca
    21361 gattttagcg agcacagccg cgcacgggat gtctttctct ggacggtgga ggatcctgcg
    21421 ggaccgatgg ataccgggtc caaaatgaag atggaaacat acaggattat cgcctcctct
    21481 ggtcaggcga tatttcaggg caaacagcag aactatgtca tgctgactgg cctatccatt
    21541 aatttccatc tccattacct cgatgcgctg aaaaagaacc tgattgcgat tgccgtcgtg
    21601 ataagcctgt tgattgttct gatcattcga atcgctgtcc gtcaggggca cctgcccctt
    21661 cgtaatgtca gcaatgccat taaaaacatc acctccgaga atcttgatgc gcgactggaa
    21721 ccgacacgcg ttcccattga gctggagcaa ctggttatct cgttcaatca tatgattgga
    21781 aagattgagg atgtctttac ccgccaggcc aatttctctg ccgatatcgc gcatgagatc
    21841 agaacgccca tcaccaatct ggtgacgcag actgaaatcg cactgagtca ggatcgaaca
    21901 cagagggaac ttgaggatgt cctctattcc agtcttgaag agtataaccg gatgaccaaa
    21961 atggtcagcg atatgctgtt cctggcacag gcagataata atcagctgat acctgacagg
    22021 gtcatgtttg acctcagagc ggaagtcatg aaagtcttcg agtttttcga agcctgggcc
    22081 gaagaacgca atatcacgct caaatttaac gggatgccct gcctggttga gggagatcca
    22141 caaatgttca gaagggcgat caataatctg ttatccaatg ccctgcgtta taccccggag
    22201 ggacaggcaa tcaccgtctc aataagagag caggagagct tttttgacct tgtgattgaa
    22261 aatccgggga aaccaatccc tgaagagcat ttatcaaggt tgtttgaccg tttttatcgg
    22321 gtggatccgt ccagacaacg aaaaggagaa ggcagcggca tcggccttgc gattgtgaag
    22381 tcaatcgtgg aagcacatca cggaagagtg caggtggaat cggacgtaca ctcaacgcgt
    22441 tttatcttat ccgtgcccag actggagaaa atgattccgg acacccagtg ctgggaataa
    22501 agatttaaat gacaaagatg tcattagcct gtcatgcagc aaacagaagc cattcgatat
    22561 aattagtgca acttatcagg aaggctggat tgctttatca atatccggcg tcagtggacg
    22621 ccaggaaatg aatatccagt cccctcccgg agaaataaac acttccgaac ctgtttcagt
    22681 tatggaactg aaaacaccgg ttgtactccc ccggacatca ctaattaaga aatggagagt
    22741 tatcatgaaa aatatcgtat tagcatcctt gctgggcttt ggcttaattt cttcggcctg
    22801 ggccactgaa accgtgaata tccatgagcg ggtcaacaat gcacaggcac ctgctcacca
    22861 gatgcagtct gctgcggctc ctgtcgggat ccaggggact gcacctcgta tggccggtat
    22921 ggaccagcat gaacaggcca ttattgctca tgaaaccatg acgaacgggt cggcggatgc
    22981 gcaccagaaa atggtggaaa gtcatcagag gatgatggga agtcagaccg tttcccctac
    23041 cgggccgtcg aagtcattag cggcaatgaa tgagcatgaa agagctgcag ttgcccatga
    23101 atttatgaat aacggtcagt ctggcccaca tcaggccatg gccgaagcgc atcgtcgcat
    23161 gctcagtgca ggctgacggc gagggaagta tctgtgctgt ccttatttcc ttgatagatt
    23221 tttccgtaac cggatatatg gcaacatctt tgccccctac aggggcattt ttatcagatt
    23281 ctgctacagt taacgggcat caacgctaaa aggatatttt ttatgaaagc cagaaacact
    23341 ttatttgcag tattaatgtt atccctgcca gcgatttcag cagaacattc agaaatgaaa
    23401 atgactgata tgtctacctc ggcttcgtca caggaatata tggctggcat gaaagacatg
    23461 catgacaaaa tgatggctgc cgtaaatgag tccgatcccg acaaggcttt tgcgaaaggc
    23521 atggtagcgc accatgaagg ggcaatagca atggctgaga ccgagctcaa atacggaaaa
    23581 gatccgaaaa tgagaaagct cgcgcaggac atcattaaag ctcaaaaagg tgaaattgag
    23641 cagatgaata aatggcttga tagtcaaaag taaacattgt agaaatgtca cgacaagcgt
    23701 gattttacat cgttcctggg cgcgggagtc ggttaatctt tatataattc aaacccgatt
    23761 aatttcgggt ttttactact ctgtactccg gtcctttgtt gaggaccgga atatatgcac
    23821 ctgagatgct tatttaatac attatctcgt catggataca atgtcgctca ggatctcaac
    23881 cagctcgctt ctctcctgcg ttccgtcatg ataggttgat cttgagatga ttctttctga
    23941 tgtcatctga ggccagaatt tatctagtac attatcgggt ttcattccca cagcccggcc
    24001 gattattttt ttagcgtcac aggtgtccgt aaacgtgacg gctacaccag taatttcctc
    24061 gataagatcc gggcacagat agttttctat ctcacgtttg cgcgtagcga aaaatacctt
    24121 accggcctcc tctacttctt ttttacgctt ctggatggac agaacctgtg cagggtctcc
    24181 cccaatatcg gagtccagaa atacgcacca gggaagcccc agatctttgg ccagattcaa
    24241 tgtaacccag tgtttgacgc taccacaccc tcctatgagg actggcacta ttttcacgtc
    24301 ctccagagag gctggcagcg cacctgactg ttttaatgaa ctggccgcat gcctcaggaa
    24361 agtaacatcc gactttccct ctaccagaac aatcccctgc gccctttcca taccggtctc
    24421 tggcagcacc cccaggcttt cagtggcttc cttcagcact gcgtcatccg gcatttttac
    24481 tacgggttca cccgcctcat ttcgggtaac ataacgtacg ccttcgacag ggattaatcc
    24541 ggccagcgcc gggacatggg tggtgacgat aatctgacgg tgcggctggc ctgccagtgc
    24601 cagtaacgct ttcatcagca tcatctgata gttcggatgc tgtgacgttt caggctcctc
    24661 tatggcataa atcacatttc tgggcgtccc cgcgacattc ttttcagcct cagcccgaaa
    24721 aaaattcaac aggataagcc tccttatccc gctgccgcgc ttattgatgg ggatgccatt
    24781 ttccccgtcc agggtgaaat tgaaggtcca cttgggtttc tccttaaatc gtggagtcag
    24841 ttcactggcg agttcggggg ccatttcacg taatttatcc agcgttctct gtgcgacatc
    24901 caggacgctg tcctgaatct cattttccag cgctgtaatt ttgtcctgca gcgcagcctg
    24961 agcgtctttt acggcctgct gtaaggggtt tttagcttcg gaatccccgt cgctgctttc
    25021 cctgtcggct ttgaaaatcg catacgtggg cagcaaatcg aggatcttac cccataatga
    25081 ttttgtgtcg gttccgaact ctttatcgac atccagcata atttccgaac aggtataggg
    25141 ggctgcggct tccctgatgg cctgacgcca taacgatgca gtgcgtttat ccgccacatt
    25201 tttttcaact tcttttccca ccgccttgag ctcagaaatt ttcatgccca gtaaatttct
    25261 caggggctcc tcatccgggt ggatgcagcg gatgcaggtc tgctccggtt ttcctgaggt
    25321 cgttgcttta aacgttttga cgatttcaaa attgccgtcc tcattcagca gatgttctga
    25381 ggcgagcgtg gtctgcaccc tgtcgtcgat catgataaaa tcgggcaggt catcaaattc
    25441 acaggctatt tcaaaacggc cgtccccttc tcttaggctg aaacagttca tgtcattctt
    25501 gtcagccttc attccttctg tttcaaaaaa aatagccagc gcttccagaa gcgttgattt
    25561 cccaaagtca ttacgaccga caatacccgt catgctttca tcaataatga tttcagtaga
    25621 gtttctgtag cctctgaagt ttttcagttt taattttctg agccgcatat gaagtcctgt
    25681 gtgtaagaaa gcgttttgct gcaacctgaa tacaaaatat attttcaaat gcctgccaac
    25741 aagcagggat aaccgttatt cagaatatct taacgacatt tcgggattat gatctggtcg
    25801 cgctttcaga actttactgc ccctcaaaac accggaaact aacttgtggt tggttacatg
    25861 gcaaagttaa aactccagaa tcacgggtaa tgatttcaat gcttattata ttttttggaa
    25921 ttgactcatg agctttataa tttaaatgat aataataacc attattttat gcgtaatatt
    25981 tgctactgac actgat
//
LOCUS       DAFNBJ010000061        25977 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000061 DAFNBJ010000000
VERSION     DAFNBJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.061"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(36..116)
                     /gene="shoB"
                     /locus_tag="L2X49_002360"
     CDS             complement(36..116)
                     /gene="shoB"
                     /locus_tag="L2X49_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBN4797440.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(537..917)
                     /gene="acpS"
                     /locus_tag="L2X49_002361"
     CDS             complement(537..917)
                     /gene="acpS"
                     /locus_tag="L2X49_002361"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBN4797441.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGDRLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(917..1648)
                     /gene="pdxJ"
                     /locus_tag="L2X49_002362"
     CDS             complement(917..1648)
                     /gene="pdxJ"
                     /locus_tag="L2X49_002362"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBN4797442.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1660..2388)
                     /gene="recO"
                     /locus_tag="L2X49_002363"
     CDS             complement(1660..2388)
                     /gene="recO"
                     /locus_tag="L2X49_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBN4797443.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2400..3305)
                     /gene="era"
                     /locus_tag="L2X49_002364"
     CDS             complement(2400..3305)
                     /gene="era"
                     /locus_tag="L2X49_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBN4797444.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3302..3982)
                     /gene="rnc"
                     /locus_tag="L2X49_002365"
     CDS             complement(3302..3982)
                     /gene="rnc"
                     /locus_tag="L2X49_002365"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBN4797445.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4254..5228)
                     /gene="lepB"
                     /locus_tag="L2X49_002366"
     CDS             complement(4254..5228)
                     /gene="lepB"
                     /locus_tag="L2X49_002366"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBN4797446.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGLRLSRIGGIH"
     gene            complement(5244..7043)
                     /gene="lepA"
                     /locus_tag="L2X49_002367"
     CDS             complement(5244..7043)
                     /gene="lepA"
                     /locus_tag="L2X49_002367"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBN4797447.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7241..7720)
                     /gene="rseC"
                     /locus_tag="L2X49_002368"
     CDS             complement(7241..7720)
                     /gene="rseC"
                     /locus_tag="L2X49_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBN4797448.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7717..8673)
                     /gene="rseB"
                     /locus_tag="L2X49_002369"
     CDS             complement(7717..8673)
                     /gene="rseB"
                     /locus_tag="L2X49_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBN4797449.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLARQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8673..9323)
                     /gene="rseA"
                     /locus_tag="L2X49_002370"
     CDS             complement(8673..9323)
                     /gene="rseA"
                     /locus_tag="L2X49_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBN4797450.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9356..9931)
                     /gene="rpoE"
                     /locus_tag="L2X49_002371"
     CDS             complement(9356..9931)
                     /gene="rpoE"
                     /locus_tag="L2X49_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBN4797451.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9928..10083)
                     /gene="rseD"
                     /locus_tag="L2X49_002372"
     CDS             complement(9928..10083)
                     /gene="rseD"
                     /locus_tag="L2X49_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBN4797452.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10339..11961
                     /gene="nadB"
                     /locus_tag="L2X49_002373"
     CDS             10339..11961
                     /gene="nadB"
                     /locus_tag="L2X49_002373"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBN4797453.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11946..12683)
                     /gene="trmN"
                     /locus_tag="L2X49_002374"
     CDS             complement(11946..12683)
                     /gene="trmN"
                     /locus_tag="L2X49_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBN4797454.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12815..14149
                     /gene="srmB"
                     /locus_tag="L2X49_002375"
     CDS             12815..14149
                     /gene="srmB"
                     /locus_tag="L2X49_002375"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBN4797455.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14358..15239)
                     /locus_tag="L2X49_002376"
     CDS             complement(14358..15239)
                     /locus_tag="L2X49_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4797456.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            15342..15929
                     /gene="eamB"
                     /locus_tag="L2X49_002377"
     CDS             15342..15929
                     /gene="eamB"
                     /locus_tag="L2X49_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBN4797457.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQQLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(15985..16368)
                     /gene="grcA"
                     /locus_tag="L2X49_002378"
     CDS             complement(15985..16368)
                     /gene="grcA"
                     /locus_tag="L2X49_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBN4797458.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16673..17362
                     /gene="ung"
                     /locus_tag="L2X49_002379"
     CDS             16673..17362
                     /gene="ung"
                     /locus_tag="L2X49_002379"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBN4797459.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(17410..18447)
                     /locus_tag="L2X49_002380"
     CDS             complement(17410..18447)
                     /locus_tag="L2X49_002380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBN4797460.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18654..19073
                     /gene="trxC"
                     /locus_tag="L2X49_002381"
     CDS             18654..19073
                     /gene="trxC"
                     /locus_tag="L2X49_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBN4797461.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            19142..19840
                     /gene="tapT"
                     /locus_tag="L2X49_002382"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             19142..19840
                     /gene="tapT"
                     /locus_tag="L2X49_002382"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBN4797462.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19872..22532
                     /gene="pat"
                     /locus_tag="L2X49_002383"
     CDS             19872..22532
                     /gene="pat"
                     /locus_tag="L2X49_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBN4797463.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22646..24001
                     /gene="pssA"
                     /locus_tag="L2X49_002384"
     CDS             22646..24001
                     /gene="pssA"
                     /locus_tag="L2X49_002384"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBN4797464.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            24047..24370
                     /locus_tag="L2X49_002385"
     CDS             24047..24370
                     /locus_tag="L2X49_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBN4797465.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24367..25665)
                     /gene="kgtP"
                     /locus_tag="L2X49_002386"
     CDS             complement(24367..25665)
                     /gene="kgtP"
                     /locus_tag="L2X49_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBN4797466.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT         6271 a         6867 c         6482 g         6357 t
ORIGIN      
        1 gttcaccgga cgcaccttaa gtacgtttcc ttgtgttata agaacagaag gatcagctgt
       61 aaaacagcaa tgatgatttt gatgacccgt ttaatcaggt atcggcaatc agtcattcgt
      121 ttttccttaa acaaggaatg cagccgttca gattagccct tacatctccc caaaactgaa
      181 cgtgcgagtt attgagggtg catgctgcac tccacaccag agctttgacg acaccactcg
      241 tttcaatggg ggaattctgt ggcatggtgt aaagcacagc aaaatcttca ataacgaagc
      301 caattttaat gtacttacga attggcggtc acgttaatct ttccatcaat attgctttct
      361 tcgtaaaggc tcgagttttt atgctaaaga ttgcaagttg cttgtaaaag ataagtacac
      421 tgatccataa tcgctgttgt tgagggtgca tgctgcacaa aattaaagtt aaaaagtaaa
      481 acccccgttc cttaccagtt cgggggtttt actttttaaa gagaacggta ttatttttaa
      541 ctttcaataa ttaccgtggc acaagcatag tgccgctcat ctgccagcgt tacatgcata
      601 tttgcaacgc ccagcttttc cgccagtttt aatgcctcgc cccatagccg tagccgtggt
      661 ttgccgagct catcattgaa tacttcaaat tgattaaacg ccagaccatt gcggatcccg
      721 gtgccaaacg cttttgctgc ggcttctttc acagcaaaac gcttcgccag aaaacgcacc
      781 ggctggtggt gcgttttcca gatagcccat tcgttatcgc ttaatacgcg gcgtgccagg
      841 cgatcaccgg atcgggcgat caccgcttcg atgcgagcga tctccacaat atccgtgcct
      901 aaacctaata ttgccattag ccacgcgctt ccagcatcag acgcttcatt tctgccaccg
      961 catctttcag tccggtcatc actgcacgac caataatggc atgaccgata ttcagttcat
     1021 gcatctcagg gatggcggca atggctttca cgttgtgata ggtcagaccg tgtccggcgt
     1081 taactttcag accgaggctt gcggcaaagg tcgcggcttt ggcgatacgc gccagctctt
     1141 gcgcctgttc ggcgtcagtt ttggcatcag catagcaacc ggtgtggatc tcgataaacg
     1201 gtgcgccaac ctctgccgca gctttgatct gctcttcatc ggcgtcaata aacagagaaa
     1261 cctgaatccc ggcatctgcc agacgtttgc aggcatcgcg cattttgtca cgctgccctg
     1321 cgacatccag gccgccttcg gttgttactt cctgacgctt ttccggtacc aggcagcaaa
     1381 aatgtggctt cgtctcaacg gcgatcgcca gcatctcttc ggtcaccgcc atctccagat
     1441 tcatgcgggt atccagcgtc tgacgcagga tgcgcacgtc gcggtcagta atgtgacggc
     1501 gatcttcacg taaatgcacg gtaatgccgt ccgctcccgc ctgctcggca ataaacgcgg
     1561 cctgcaccgg atccgggtaa gcggtaccgc gcgcgttgcg cagcgtagcg atatggtcaa
     1621 tgttgacgcc taacagtaat tcagccatga caatcctcat cattcataat gtgttttcac
     1681 cgttcgctta ggcataaatt gccggaacag ttccctgctc tttaaaggtt taccgccaag
     1741 atacggctta agcgccatgc gggtaaagcg tttcgcagcg cgcagtgtgt ctgcgtcagg
     1801 aaattcccgt gcgtttaacg cttttaactg ccttccggtg aacgttttat tgtcgataac
     1861 gacgcttgcg ataaaccctt tttcttcgcg ataacgatac gtcatggtgt catctaccgg
     1921 ctcgccgcta cccgcacaat gggtaaaatt gacgccataa cccagatgcc cgagcagtgc
     1981 cagttcaaag cggcgcagcg cgggttctgg cgtaccagtg acccctgcaa gagactgaat
     2041 gcagtgcaag taatcgaaaa aaagttcaga gaagcgcgtc tcgtattcca gtacgcggga
     2101 gagaagttcg ttgatgtaca gaccgctgta aagcgtgata ccgcttaatg gcagcgccag
     2161 cgagacggct tcagcactgc gcagcgtttt gacttcgcca cgcccgccaa aacgtagcaa
     2221 gagaggggtg aaaggctgta atgcaccttt cagggtagag cgtttagagc gtgcgccttt
     2281 ggcaaccaga cgcacgcgcc ccgattcctc cgtgaagacg tccagcatca ggctggtttc
     2341 gctccacggg cgactatgca ggacaaatgc gcgctgccag ccttccatcg gagttactct
     2401 taaagatcgt caacgtaacc gagactgcgc agtgcgcgtt cgtcgtcggc ccaaccggat
     2461 ttcactttta cccacagttc aaggtgaaca ggcgcttcga acatttcctg catgtcttta
     2521 cgcgcttcaa tcccgatggt tttgatcttg gcccctttgt tgccaatgac catcttcttc
     2581 tgcccttcac gctcaacgag aatcaaaccg ttgatgtcat aaccaccgcg ttcgttagag
     2641 acgaaacgtt cgatctccac ggtcacggag tacggcagtt cagcgccgag gaaacgcatc
     2701 agtttttcgc ggatgatttc agacgccata aaacgctgtg agcgatcggt gatgtaatct
     2761 tccgggaagt gatgagtcgc ttcaggtaga tgcttacgca cgattgccgc aatagtgtca
     2821 acattcagcc cggtttcggc agagattggc acgatatcga ggaagttcat ctggcttgcc
     2881 aggaactgca ggtgcggcag cagatcggct ttctcctgca cgttgtccac tttgttcacc
     2941 gcgaggatta ccggcgcttt gccttcgcgc agtttgttga gcaccatttc gtcgtccggc
     3001 gtccagcggg tgccttcaac gacaaaaatc accagctcaa catcgccaat agagctgctc
     3061 gccgctttgt tcatcaggcg gttaatggcg cgtttttctt ccatatgcag gcccggtgta
     3121 tcgacgtaga tcgcctgata cgcgccttca gtatggatcc ccacaatgcg gtgacgagtt
     3181 gtctgcgcct tgcgggaagt gatggagatt ttctgcccca gcagtttgtt caacaatgtg
     3241 gatttgccaa cgttcggacg tccgacgatg gcaataaatc cgcagtaact tttatcgatg
     3301 ctcattccag ctccagtttt ttcaacgcct gttcggcggc agcctgctca gccttacgac
     3361 ggcttgaacc tgtgccaacc accggttcac tcaggccgct gacctggcag tggatagtaa
     3421 attcctgatc gtgcgcttcg ccacgtacct ggactaccag ataagtcggc agcggcagat
     3481 ggcgaccctg caaatattct tgcaagcgcg ttttcggatc tttttgttta tcgcctgggc
     3541 taatttcgtc caaacgagtt tgataccagt tgaggattaa tttctcgacg gtttgaatat
     3601 cactgtcgag gaatacgcca ccaattaatg cttcgacggt gtcggcgaga attgactcac
     3661 gacgaaatcc accgctttta agttcacctg gccctaaacg taagcactcg cctaactcaa
     3721 attcgcgcgc cagttccgcc agcgtattgc cacggaccag cgtggcgcgc atccggctca
     3781 tatcgccttc atccacacga gggaaacggt gataaagcgc attggcgata acgtagctca
     3841 gaatagagtc gcctaaaaat tctaaacgct cgttatgttt actgctggca ctacgatgag
     3901 ttaatgcctg ctgcaacagt tcctgatgat taaaagtgta gcccagcttc cgttgaagcc
     3961 gattaattac gatggggttc atgcgatacc aataaataaa tgcgtcaaca attcagcaca
     4021 cgaaacagac ctgatataca tggttctgct aactgcttcg ctgcagtttc agtttaccgg
     4081 tatatgggga ccaacgctgt ttcgtgtgcc gtggcaacct ggaggtgcca accttaaact
     4141 tcgggggaat attctataca caacgacggg ggatgtcgtt agccacggga gatttatctc
     4201 ataaataatt cacgttgtcg ccataacggc gacaacgtga acgaagatgg ctattaatgg
     4261 atgccgccaa tgcgacttaa gcgcagacca gtcggccatt cgccttcttg cttatcgaag
     4321 ctcatccaga tagccgttgc ccgaccgacc agattcgctt ccggcacaaa gccccagtaa
     4381 cggctgtccg cgctgttgtc gcggttgtcg cccatcatga agtattgtcc cggaggaaca
     4441 atccaggttg ccagttgttg ccctggctgc tggtaataca tccccacctg atcctgcgca
     4501 atcggcactg tcagaatgcg gtgcgtcaca tcacccagtg tctctttacg ctcggaaaga
     4561 cgaattccat tttctttggt ttcgtttttc ggcacttcaa agaatccgct ggtcgcttcc
     4621 ccaccattac ggcgtgagaa ggtctgaacg aaatcgctcg gttccacgtt tgagtaggtg
     4681 accggcagcg cgttttcaca cgcctggccg gaactgcatc ccggttgaat cgtcagctct
     4741 tttgagaccg gatcgtaagt gactttatcg cccggtaaac ccaccgcgcg cttgatgtaa
     4801 tcaagctttg gatcttccgg atatttaaag accacgatat cgccgcgttt cggatgaccg
     4861 gtttcgatca gcgttttctg gtagatagga tctttaatgc cataagcaaa cttctctacc
     4921 agaataaaat caccaattaa cagagtcggc atcatcgaac ctgacgggat ctggaacggt
     4981 tcataaataa acgaacgcac aatcaatacg atagccagta ccggaaaaac agaagcaccg
     5041 gtttccagcc agccaggctt cggcgcaacc tttttcaacg ttgctttatc cagtgagtca
     5101 ccggcagccg cctgcgccgc tgcctgacgt tcccgccgtt taggtgcgaa aaagaattta
     5161 tccacgcacc ataaaatgcc cgtcaccagt gtggcaatca ccagaatcag ggcaaacata
     5221 ttcgccatgc caactcctaa gggttatttg ttgtctttgc cgacgtgcag aatggcgagg
     5281 aacgcttcct gcggcagctc gacgttaccg atctgcttca tgcgtttctt accttctttc
     5341 tgcttctgca gcagcttttt cttacggctg atatcgccgc cataacattt agccagtacg
     5401 tttttacgca gctgtttcac ggtggatcgc gcaataatgt gcgtaccaat cgctgcctga
     5461 atggcgatat caaactgctg gcgtgggatc agatctttca tcttctccac caactcgcga
     5521 ccgcggtttt gcgaattatc acggtgggtg atcagcgcca acgcatcaac acgttcaccg
     5581 ttgattaata cgtctacacg taccatgtcg gacgcctgga agcgtttgaa gttgtaatcc
     5641 agagacgcat aaccacgcga ggtagatttc aggcgatcga agaagtcgag caccacttcc
     5701 gccatcggga tctcgtacgt cagcgccacc tgattaccgt ggtaaaccat attggtctgc
     5761 acgccgcgtt tttctacgca cagcgtaata acgttgccga gatatgcctg cggcagcagc
     5821 atgtgacatt cggcaatcgg ctcgcgcagt tcgtagatgt tatttaccgc tggcagcttg
     5881 gatggactgt cgacgtaaat aacttctctt gacgtggttt caacttcata cactacggtc
     5941 ggcgcagtgg tgatcagatc cagatcgtat tcacgttcca gacgttcctg gatgatctcc
     6001 atgtgcagca ggccgaggaa gccgcagcgg aaaccaaagc ccagcgcgct ggagctttcc
     6061 ggctcataga acagtgaagc atcgttcagg ctgagtttac ccagcgcgtc acggaaggct
     6121 tcatagtcgt cggaacttac cgggaacaga ccggcgtata cctgcggttt gactttctta
     6181 aagccaggca gcgccttttc tgccggatta cgcgccagcg ttaaggtatc gccgactgga
     6241 gcgccgtgga tatctttaat cgcacatacc agccagccta cttcgccaca tttcagttca
     6301 gtgcggtcaa cctgcttcgg cgtgaagatg cccagacggt cggcgttata ggtctgcccg
     6361 gtactcatga ctttcacttt gtcgccctta cgcagggtgc cgtttttaat acggataagt
     6421 gaaacaacgc ccagatagtt gtcgaaccag gagtcgataa ttagtgcctg caacgggcct
     6481 tccggatcgc cttccggcgg cggaatgtcg cgcaccagac gttcgagaac gtcctgcacg
     6541 ccaacgccgg ttttcgctga acagcgcacc gcgtcggtgg cgtcgatgcc gacgatatct
     6601 tcaatttctt ccgccacgcg ttcaggatcg gctgccggca ggtcaatctt gttcagtacc
     6661 ggcaccactt cgagatccat ttccatggcg gtgtagcagt ttgccagggt ttgcgcttct
     6721 acgccctgcc cggcgtcgac caccagcaat gcaccttcac aggcagccag cgaacgggaa
     6781 acttcatagg agaagtctac gtggcccggg gtgtcgataa agttaagctg ataggtttcg
     6841 ccgtcagacg ctttgtagtc cagcgtcacg ctttgcgctt tgatggtaat gccacgctca
     6901 cgctcaagat ccatggaatc gagaacctgc gcctccattt cacggtcaga caggccaccg
     6961 cagatctgga taatacggtc agacagcgtc gatttaccgt ggtcaatgtg agctatgatc
     7021 gaaaagttac gtatattctt cataaagtat gattattgtg ccttaatgcc cggttaacca
     7081 ggcttttaga agtcgctgtt ctgagcttaa cgtctgtatt aatagaaacg ccgcattcta
     7141 cactacaaca ttgaggcgag gaaatgttca taccgtatgg attgtggtat ctggaaacgt
     7201 cctcgcattt gttatgcaaa atgcaataaa gccagtgaaa tcactggctc gcgtcttccg
     7261 aagatgtttc aaatcgcaca aggccaggcg gcaatgccac gcttaagatg atcggttgcc
     7321 attctgcccg ggcagcaaac ttacgcgagt agccgcgcgc aatcaggaac ccgccaatac
     7381 caccgaggat cgcaccacat aatgctgcga cgtcagaagc aaagagtagc tgaaatagcg
     7441 aagcgatgag gaataatccc accagcggcg acatataaac cagtaatgcg gagctaagca
     7501 ggctgccttc ggcgatcccc aattccactt tttgccccgg cactaacggc tcatcacagg
     7561 gtacgacaat ggtatgcgtg gtttgcgggc caagtttatt taacacgcgg ctaccgcaac
     7621 cggcgcgtga agcgcagctg ctgcatgaag ctttaacatc acaactgacc agcgcctgcc
     7681 cgttttgcca ggagacgacg gtagcccact ctttgatcat tgcgctgccc cgaacttaat
     7741 attctcggca atgcgtttcg ccgtttgcgg cggcagttca ccgacaatgg tgatttcggc
     7801 gttatcacgt acgcttgtac tgacggttct gcgtccggtg cgcaacatct gatcggtgct
     7861 cgatggcgta gcgcggttaa cgtttaccga gaagctgaat aatccgtcgg aatagagacg
     7921 tgattcgata ggcatgttgt ccatcgtcgg tagcggacgt cgactactgg aaacttcgct
     7981 aaaaccctgt ggcaaccagg ttggcgtcca gctgaattta gctttttcac ctacaggaac
     8041 agaaagcaac ggcggcaaat ttgccttcgc cagcgtctgc atactgctgc tgatatcctg
     8101 attgacgtta aaagcaatca cgcgaaattg ttccagcgtt tcaccatcgc gatcaaggag
     8161 atcaacccgc atcggtaatt tcgattcggt gtccatccac acgatgtagc tgtagcgtgt
     8221 accatctcgg gcaaccacgc gaatgacttc gcaaagacga tcagcaatac gcgtgcgccc
     8281 gacggagata aagtcgtagt aaggagaaag gcgtttgaaa tcggtataga taagcgatgg
     8341 cagagaatca acaatgtaat cgccattaag cgtgaacggt tcaagtcccg gttcaaaata
     8401 gctgatttca ttgccgcgct gtaccacttc ccggcgcggg ccatccattt gcaacaattg
     8461 tgcaagaggg cggttatcga ggcgtgcatg tcgataacgc agagactcaa caccctgttt
     8521 attgatgctg atgaatgaca gctcgtaatt cagtgactgc ctggccaggt tcatctgctg
     8581 taataacgcc ccggacgcgg gagtggccga ggcgttagca gagaataaca ggctacctgt
     8641 cactaatgac atggcaaacc aaagttgctt cattactgcg attgcgttcc taaagtttga
     8701 attcctggca cctgtacagc ggcttgctgg gtttgcgcct gctcaaactg aagctgttca
     8761 gagtggagtc ggcgttgcag ttcgtaatcc tgcaacattg cattaatgcg acgacgctgc
     8821 tcctgtacct gctgctgttg accattgttt gcggtcgctt cagaaggtac tcccaggctt
     8881 accgggctgg ctttacccat catcggcagt gtattaaata ccggcgtttc gggctgctgg
     8941 gacgtttcag attgtccatt atagtgctgg acgccaacga taactgcaag cgatacgcat
     9001 gctgctacgc ccatttgggt aagctgtgcc gcccacggac gtactttctg ccagaatggc
     9061 attttctgcc attgatgcgg cgcaggctgg gcttccggga tcaatgtcgc cggttgacgt
     9121 actggctctt cttcaatggc ggccatcacg cgtgaagaga tatcgaaatg gagcacctcg
     9181 ggagtatcac cccgcattga gtcacggatt aagtgatagc tttcccaggt tttctgcatt
     9241 tctgggttat gagccagttc gttaagcagc tcactatcca gcgtttcgcc atccattaaa
     9301 gcggaaagtt gttctttctg catgcctaat acccttatcc agtatcccgc tatcgtcaac
     9361 gcctgataag cggttgaact ttgttatcaa tagcttccct cgctcggaag atacgtgaac
     9421 gcaccgtacc taccggacaa tccatgatag cggctatctc ttcatagctc aggccatcca
     9481 gctcccgcaa ggttattgcc atgcgcaaat cttccgggag ggactcaata gttcggaaaa
     9541 ctatctgtct cagttcttct gacaacatta agttctcagg gttcgaaatt tctttcaacg
     9601 cgccgccact ttcgaagttt tcagcttcaa tggcatccac atcactggaa ggtggacgac
     9661 gcccctgagc aaccaggtaa tttttcgctg tatttacagc aatccgatac agccatgtat
     9721 aaaaagcgct atctccccgg aacgaatcca gcgcacgata ggctttaata aaagcttctt
     9781 gtaccacatc gggaacatca cccgacggca catagcggga aaccagactc gccactttat
     9841 gctgatagcg cactaccagt aggttaaagg ctttctgatc tcccttctgg acccgttcaa
     9901 ccaggacctg gtccgttaac tgctcgctca tccgaggtaa agtctcccca aaccaaattt
     9961 ccacgcgcta tcgaaacgcc actccattag ctgcaatttg agcaagcaaa gggttagagt
    10021 gtctcgtttt tgtaaagttc cgtaacgcat ctgtttttgt ttgtcatgct gtagacggat
    10081 cattatctat cattataagt ctacagaatc tgaacatcgc attatctgtg tagaaatgcc
    10141 catttaactg cctgaagagt aacccaacgg cctttttatt tcaccaccta atcctccacc
    10201 agccagtaac ttctcttttt ctcgccgccc tgcgtcagcg tgtttagcca ctgtaacaaa
    10261 tattaaaata gcaggtgttt attcgcacaa catgatgcta tgctgaccaa acggtgttta
    10321 gtaaattaaa caaagaaaat gaatactctc ccagaacatt catgtgacgt gttgattatc
    10381 ggtagcggcg cagccggact ttcactggcg ctacgcctgg ctgaccagca tcaggtcatc
    10441 gttctaagta aaggcccggt aacggaaggt tcaacatttt atgcccaggg cggtattgcc
    10501 gccgtgtttg atgaaactga cagcattgac tcgcatgtgg aagacacatt gattgccggg
    10561 gctggtattt gcgatcgcca tgcagttgaa tttgtcgcca gcaatgcacg atcctgtgtg
    10621 caatggttaa ttgaccaggg ggttttgttt gatacccacg ttcaaccgaa tggcgaagaa
    10681 agctaccatc tgacccgtga aggtggacat agtcaccgtc gtattcttca tgccgccgac
    10741 gccaccggta gagaagtaca atccacgctg gtgagcaagg cgcagaacca tccgaatatt
    10801 cgtgtgctgg agcgcagcaa cgcggttgat ctgattgtct ctgacaaaat tggcctgccg
    10861 ggcacgcgac gggttgttgg cgcgtgggta tggaaccgaa ataaagaaac ggtggaaacc
    10921 tgccacgcaa aagcagtggt actggcaacc ggcggtgcgt cgaaggttta tcagtacacc
    10981 accaatccgg atatttcttc tggcgatggc attgctatgg cgtggcgcgc aggctgtcgg
    11041 gttgccaatc tcgaatttaa tcagttccac cctaccgcac tgtatcaccc acaggcacgc
    11101 aatttcctgt taacggaagc actgcgcggc gaaggcgctt atctcaagcg cccggatggc
    11161 acgcgtttta tgcccgattt tgatgagcgc ggcgaactgg ccccgcgcga tattgtcgcc
    11221 cgcgccattg accatgaaat gaaacgcctc ggcgcagatt gtatgttcct cgacatcagc
    11281 cataagcccg ccgattttat tcgccagcat ttcccgatga tttatgaaaa attgctcggg
    11341 ctggggattg atctcacaca agaaccggta ccgattgtgc ctgctgcaca ttatacctgc
    11401 ggtggtgtaa tggttgatga tcatgggcgt acggacgtcg agggcttgta tgccattggc
    11461 gaggtgagtt ataccggctt acacggcgct aaccgcatgg cctcgaattc attgctggag
    11521 tgtctggtct atggctggtc ggcggcagaa gatatcacca gacgtatgcc tgatgcccac
    11581 ggcgtcagta cgttaccgcc gtgggatgaa agccgcgttg agaaccctga cgaacgggta
    11641 gtaattcagc ataactggca cgagctacgg ctgtttatgt gggattacgt tggcattgtg
    11701 cgcacaacga agcgcctgga acgcgccctg cggcggataa ccatgctcca acaagaaata
    11761 gacgaatatt acgcccattt ccgcgtctca aataatttgc tggagctgcg taatctggta
    11821 caggttgccg agttgattgt tcgctgtgcg atgatgcgca aggagagccg tggactgcat
    11881 tttacgctgg attacccgga actgctcacc cattccggtc catcgatcct ttctcccggc
    11941 aatcattaca taaacagata aaaagcctgg gtcagcgctg tatacgcttc ggaatagttc
    12001 tggtctggcc cacgaatgac taagcgatca ctaaagcatt ctcccgcctg cggggagaat
    12061 gccagcagca cccgatgcgg cagtcgcgct tcgttttccg ccacatccgt ccgcaaacgt
    12121 aaatgccagc ccatgcttaa tgccagttca gtaaaaccat taccaatctg ctctggcagc
    12181 accacgcaga aaaatccctc ttcggtaatg cactccgccg cacaggtcag cagcgatggg
    12241 tgatcaagcg tagtggtata gcgagcctgt tcccgttgag gtgtcgcaca ctctactccc
    12301 tgctgatagt aaggtgggtt actgatgatt aaatcgaagc gcgcggtctg ctgtgttacc
    12361 cactgctgaa tatccgccgt atggacgtta atccgctctg cccacgggga ctggttgata
    12421 ttttcctgcg cctgcgcggc agcttcactt tccagctcaa cggcatcaat tatcacgctg
    12481 tcactggttc gctgcgccag catcagtgcc agcaacccgc tacccgcgcc gatatcaagg
    12541 caacgtttca ccccagccac cggtgcccat gcgcccagca aaatgccatc cgttcccact
    12601 ttcatcgcac agcgatcgtg agcaacaaaa aactgtttaa aagtaaatcc attacgacga
    12661 agcacggatg tagactgtga catgaaaata aaaccttgca ggaaaaacgg cgatagcacc
    12721 gggtgagaac aatacccgag aagcgatatc catacaaaca gatgaagatt gcagccgtaa
    12781 cgtctataat cagcgcccca cacagaggta gaacatgact gtaacgactt tttccgaact
    12841 tgaactcgac gaaagcctgc tggaagccct ccaggataaa ggtttcactc gcccgaccgc
    12901 cattcaggct gccgccattc cgcctgcgct cgatggccgt gatgtactcg gttctgcgcc
    12961 gacaggcacc ggtaaaacgg cggcgtatct gctgccagcg ttgcagcacc tgctcgattt
    13021 cccacgtaag aaatccggtc caccgcgtat tttgatcctc accccaactc gcgagctggc
    13081 gatgcaggtg gccgatcatg cccgcgaact ggcgaaacat acgcatctgg atatcgccac
    13141 catcaccggc ggcgtagcct atatgaacca cgcggaagtg ttcagcgaaa atcaggacat
    13201 cgtggtcgcc acgaccggac gtctgctgca atacataaaa gaagagaact tcgattgccg
    13261 cgcggttgaa acgctgatcc tcgacgaagc agaccgtatg ctggatatgg gcttcgctca
    13321 ggatatcgaa catattgctg gcgaaacgcg ctggcgtaaa cagaccctgc tcttttcggc
    13381 aacgctggaa ggcgatgcga ttcaggactt tgccgagcgt ctgctggaag atccggtgga
    13441 ggtttctgcc aatccgtcca cccgtgagcg caaaaaaatt catcagtggt attaccgcgc
    13501 cgatgacctg gaacataaaa ccgcattgct ggtgcatctg ttaaaacagc cggaagcgac
    13561 ccgctcaatt gtgtttgtgc gtaagcgtga acgtgtgcat gagctggcaa actggctgcg
    13621 cgaagcgggc atcaacaact gctatctcga aggtgagatg gtacaaggca agcgtaacga
    13681 agcgatcaag cgtttgaccg aaggtcgcgt aaacgtactg gttgcaaccg atgttgccgc
    13741 gcgcggtatc gacattcctg acgtcagcca cgtctttaac ttcgatatgc cgcgcagtgg
    13801 cgatacttat ttgcaccgta tcggacgtac cgcgcgcgcc ggtcgtaaag gcaccgcaat
    13861 ttcgctggtg gaagcccatg accatttgct gctgggcaag gttggccgtt acattgaaga
    13921 gccaattaaa gctcgcgtta ttgatgagtt acgcccgaaa acgcgtgcgc caagcgaaaa
    13981 gcagaccggc aagccatcga agaaagtact ggctaaacgt gccgagaaga aaaaagctaa
    14041 agagaaagag aagccgcggg tgaaaaaacg ccatcgcgac accaaaaata ttggtaagcg
    14101 ccgtaaacca agcggaacgg gcgtgccacc gcaaacgaca gaagagtaat ctcaatgccg
    14161 ggtttaagcc tggtattaaa agtgctggca aacgcaaaac tgcctgatgc gctacgctta
    14221 tcaggcctac gtggctcatg caatatattg aatttgcacg atcttgtagg ccggataagg
    14281 cgttcacgcc gcatccggca tgaataaagc gcactttgtc gacaagttaa atgccgggtt
    14341 taatcccgac attattttta tccagccaca aaactctctt caacacactg aataaaagtg
    14401 tgcatcgccg ggctaaccgc ttttccagca tggtgtgcgc acatggccgt aatggtctgc
    14461 gactgttcgc caaaaggcaa ttcaattaat tctccgctct caagctcttt ttccaccgca
    14521 aagcggggca agtaactgac accgatattc gccgcgacac aacgtttgat actttcaatg
    14581 cttaacagct caatggtgtt ttccaccgtg atccgccgct gacgcagcgt gctctcaaat
    14641 atctgccgga agacacattg cggttcgttg ataataaagc tacaggcgtt atgtcttccc
    14701 ggctcagtaa aatcgacatc tgcaatttgc ggtgaagcca ccagcaccag cgattgttca
    14761 cccaactctc gtcggttcag ggcatcatca ttccctacgc gataaaagac gccgacatcg
    14821 gcctcatcat tcagtagcgc atcacggatc acgtaacagt tcagcgactg caacgataaa
    14881 cgcacttttg gtgcgcgttg ccgaaaacgc tgcaacacct gcggcattcg gtaagagagc
    14941 aacgtttcgc ccgaaacaac gcgtagttcc ccgtccggat ctgactcctt tttagcagcc
    15001 tcacgaagcg tatccatcac tcgggtaagt tcataaatgt gcggcagcaa cttttttcct
    15061 tcgcgggtaa ggcacatccg tcggccaatt ttctcaaata actggactga aaactcctgc
    15121 tcaagctgct gaatatgaaa agtcaccgtc gattgtgtac agcacaattt ttgcgaagct
    15181 cgcaaaaagg aaccctcttc caccacggtt ttaagcgtaa taaaacggcg cagatccata
    15241 accccaaacc tatcgaaaat atcgaatcta gaatataaaa acattcattt ttttaaatgt
    15301 tccgtgtcgg gtactgtcta ccaaaacaga ggagataaca agtgacaccg acccttttaa
    15361 gtgctttttg gacttacacc ctgattaccg ctatgacgcc aggaccgaac aatattctcg
    15421 cccttagctc tgctacgtcg catggatttc gtcaaagtac ccgcgtgctg gcagggatga
    15481 gtctgggatt tttgattgtg atgttactgt gtgcgggcat ttcattttca ctggcagtga
    15541 ttgacccggc agcggtacac cttttgagtt gggcgggggc ggcatatatt gtctggctgg
    15601 cgtggaaaat cgccaccagc ccaacaaagg aagacggact tcaggcaaaa ccaatcagct
    15661 tttgggccag ctttgctttg cagtttgtga acgtcaaaat cattttgtac ggtgttacgg
    15721 cactgtcgac gtttgttctg ccgcaaacac aggcgctaag ctgggtagtt ggcgtcagcg
    15781 ttttgctggc gatgattggg acgtttggca atgtatgctg ggcgctggcg gggcatctgt
    15841 ttcagcaatt gtttcgccag tatggtcgcc agttaaatat cgtgcttgcc ctgttgctgg
    15901 tctattgcgc ggtacgcatt ttctattaac gaaaaaaagc ggaagaggtc gccctcttcc
    15961 gcttagtaac ttgctactta agccttacag gctttcagta aaggtacgag cgataacgtc
    16021 gcgctgctgt tccggagtca gagagttaaa gcgaactgca taaccggata cacggatggt
    16081 cagctgcgga tatttttccg gatgcttaac tgcatcttcc agagtttcac gacgcagaac
    16141 gttaacgttc aggtgttgac caccttcaac gcgaacttct ggtttcactt ctactggaac
    16201 ttcacggtat tcaatgtcac ccagtttgct tactgcaacc acttcatctt ctgcataacc
    16261 tgcttttgca acgatgcaac gtgcttcgcc tttttcgctg tccagcagcc agaaagagtt
    16321 cagcagatcg tcgttagcgg ctttagtaat ctggatacct gtaatcatgt gatgcctccc
    16381 cggcaaaatt atttgatttg ttcagcctgt cgcggccaat tggtaaaacc attgttgctt
    16441 gagtgtatat atactcctca accacccttg attctttgat ttaaatcaat aaaaaccaca
    16501 catcgattgt ggtcgtaaat ggattttatt gttttacatc aacttatgcg ggtgtgaaat
    16561 tttaccaatt tacatttttt tgcactcgtt taagtctaaa aaatgagcat gattttgttc
    16621 tgtagaaaga agcagttaag ctaggcggat tgaagattcg caggagagcg agatggctaa
    16681 cgaattaacc tggcatgacg tgctggctga agagaagcaa caaccttatt ttcttaatac
    16741 ccttcagacc gtcgccagcg agcggcagtc cggcgtcact atctatccac cacaaaaaga
    16801 tgtctttaac gcgttccgct ttaccgagtt gggtgacgtt aaagtggtga ttctcggcca
    16861 ggatccttat cacggaccgg gacaggcgca tggtctggca ttttccgttc gtcccggcat
    16921 tgccattcct ccgtcattat tgaatatgta taaagagctg gaaaatacta ttccgggctt
    16981 cacccgccct aatcatggct atctcgaaag ctgggcgcgt cagggcgttc tgctgctcaa
    17041 tactgtgttg acggttcgcg caggtcaggc gcattcccac gccagcctcg gttgggagac
    17101 gtttactgat aaagtcatca gcctgattaa ccagcaccgc gaaggcgtgg tgtttttgtt
    17161 gtggggatca catgcgcaaa agaaaggggc gattatagat aagcaacgcc atcatgtact
    17221 gaaagcaccg catccgtcgc cgctttcggc gcatcgtgga ttctttggct gcaaccattt
    17281 tgtgctggca aatcagtggc tggaacaacg tggcgagacg ccgattgact ggatgccagt
    17341 attaccggca gagagtgagt aaatttgcgg taaaatgccg gatggcagag ttgccacccg
    17401 gctgatttat caggctttat tctgacgcca ccattcacca agcaaaacgc cggttgcaac
    17461 agagatattc agcccggcaa cgttgcccgt accgtcaatc ttcacgcgca gatcgttcgg
    17521 atcgcgtgcg gcatccggta acccttcata ttcctgaccc agcaccagta ccattttcgc
    17581 tggcagactg gttttgaaca gcggtttccc ctgctcgctg gaagtggtca ctacggtgta
    17641 acctgcctga cggaaatcat ccaacacgtt aacaatgttg tcgccagtaa tcggctgaac
    17701 gtgctctgcg ccgccttctg cggtacggat agccgccccc gactccagca gtgccgcatc
    17761 ctgcaccaca acgcctttca cgccgaagtg cgcgcagctg cgcatcatgc cacccaggtt
    17821 atgcgggtta gattcgtttt ccagcgccag tacacaatcc tgcgcgcctg cctggcttac
    17881 ccactgctgc acggttgtgc cattacgctt tttgatcagg aagcaaacgc cgccgtgatg
    17941 ttccgtgcct gacgcttttg tcaattccgc ttcatccacc acatggtacg ctttgcggtt
    18001 tgctgccatc cagcgcaagg cttctttaaa acgcggcgtt acactctgga taaaccaggc
    18061 gcgaacaatc gcttccggac ggctctggaa aagtgcctga catgcatttt cgccgtagac
    18121 gcgggtttct tccgcacgct gacgacgcaa cacttccgga tcaataaaac ttttaccact
    18181 gatgccaccg tgatcggcct tttccggcgt ctcatcaccc ggcgcgcggg aaaccgtgcg
    18241 ccacggcgaa tcttcccact tgcggtcacg gggctgactc tgtttgtcat cgcgggcggg
    18301 gcgacggcca ccgtcagcac gagattttcc tggacgcccg ccccctttcc cggtacgcgg
    18361 gttgtgggta cgtttatcag aatcatcatc actgcggaca tacatcactt tgaccttgcc
    18421 gcttttacct ttcatttcat cgttcatgct tttctccacc agcgctgcgc gaagcgcgca
    18481 gattacccga agtccgcgcg gttcgccatg atttcgtacc aaagcctgcg actatcatac
    18541 ctattgaata aaacagattg ttgtctggaa caatgtcccc gataatatgt aacatattag
    18601 aaacataccg gcgtcgttgc cgataagtct ccttactcat cccgaggtta gttatgaata
    18661 ccgtttgtac ccattgtcag gccatcaatc gcattcccga cgatcggatc gaagatgcgg
    18721 caaaatgcgg acgctgcggt cacgacttgt ttgacggaga ggtgattaat gcgaccggtg
    18781 aaacactcga caaattgctg aaggatgatc tacctgtggt gatcgacttc tgggcaccgt
    18841 ggtgcggccc ctgccgtaat ttcgcaccaa tttttgaaga tgtcgcgcaa gagcgtagcg
    18901 gtaaagtgcg ctttgtgaaa gtgaataccg aagctgaacg tgaattgagc agtcgctttg
    18961 gaattcgtag tataccgacg atcatgattt tcaaaaacgg tcaggttgtc gacatgctta
    19021 atggcgcagt accgaaagcg ccgttcgata gctggctgaa cgaatctctt taatcttacc
    19081 ggggcgcatc ttgtgccccg ttttctcctc tgcgacaatg gcgttttttc gacgctctct
    19141 tatgaccgaa aacgctgttc tccagttacg cgccgagcgt attgcgcgcg caacacgtcc
    19201 ttttcttgcc cgcggtaatc gcgttcgtcg ctgccaacgc tgtcttttgc cagagaaatt
    19261 atgtctctgt tcgacaatta ccccagcaca agcaaaaagt cgcttctgtt tgctgatgtt
    19321 cgacaccgag ccaatgaagc ccagtaatac cgggcgtctg attgctgata ttttgcctga
    19381 taccgttgcg tttcaatggt cgcgtaccga accctcgcaa gatttactgg atctggtaca
    19441 aaacccggac tatcagccaa tggtggtctt tcccgcttcg tatgctgatg agcaacggga
    19501 agtgatcttc acaccacctg ccggtaagcc accgctgttt atcatgctcg atggtacctg
    19561 gccggaagct cgcaagatgt ttcgtaaaag tccgtatctg gataatcttc ccgtcatttc
    19621 cgtcgatctt tcccggcttt ctgcctatcg cctgcgtgaa gcccaggctg aaggccaata
    19681 ttgtactgcc gaggtagcca tcgcactgtt agatatggcc ggcgataccg gggcggcagc
    19741 agggttaggc gagcatttta cccgctttaa aacacgctat ctggcaggaa aaacgcaaca
    19801 tctgggtagc atcacagcag aacagttaga aagcgtttaa aatcattcgg tcacttctgc
    19861 gggagaccgg tatgagtcag cgaggactgg aagcactact gcgaccaaaa tcgatagcgg
    19921 taattggcgc gtcgatgaaa cccaatcgcg caggttacct gatgatgcgt aacctgctgg
    19981 cgggaggctt taacggaccg gtactcccgg tgacgccagc ctggaaagcg gtgttgggtg
    20041 tgttggcctg gccggatatt gccagcttgc cctttacacc cgaccttgcg gttttatgta
    20101 ccaatgccag ccgtaatctt gctcttctgg aagagctcgg cgagaaaggc tgtaaaacct
    20161 gcattattct ttccgccccg gcatcgcaac acgaagatct ccgcgcctgc gccctgcgcc
    20221 ataacatgcg cctgcttgga ccaaacagtc tgggtttact ggctccctgg caaggtctga
    20281 atgccagctt ttcgcctgtg ccgattaaac gcggcaagct ggcgtttatt tcacaatcgg
    20341 cagccgtctc caacaccatc ctcgactggg cgcaacagcg tgagatgggc ttttcctact
    20401 ttattgcgct cggcgacagc ctggatatcg acgttgatga attgctcgac tatctggcgc
    20461 gcgacagtaa aaccagcgcc atcctgctct atctcgaaca gttaagcgac gcgcgacgct
    20521 ttgtttcggc agcccgtagt gcctcgcgta ataaaccgat tctggtgatt aaaagcggac
    20581 gtagcccggc ggcacagcga ctgctcaaca cgacggcagg aatggacccg gcatgggatg
    20641 cggctattca gcgtgccggt ttgttgcggg tacaggatac ccacgaactg ttttcggcgg
    20701 tggaaaccct tagccatatg cgcccgctgc gtggcgaccg gttgatgatt atcagcaacg
    20761 gtgctgcgcc tgccgcgctg gcgctggatg ccttatggtc acgcaatggc aagctggcaa
    20821 cgctaagcga agaaacctgc cagaaactgc gcgatgcact gccaggacat gtggcaatct
    20881 ctaacccgct cgatctgcgc gatgacgcca gcagtgagca ctatattaaa acgctggaca
    20941 ttctgctcca cagccaggat tttgatgcgc tgatggttat tcattcgccc agcgccgctg
    21001 ctcccgcaac agaaagcgcg caagtattaa ttgaagcggt aaagcatcat ccccgcagca
    21061 agtatgtttc tctgctgacg aactggtgtg gcgaacattc ctcgcaagag gcacgacgat
    21121 tattcagcga agccgggctg ccgacctacc gtaccccgga aggaaccatc actgctttta
    21181 tgcatatggt ggagtaccgg cgtaatcaga agcaactacg cgaaacgccg gcgttgccca
    21241 gcaatctgac ctccaatacc gcagaagcgc atcttctgtt gcaacaggcg attgccgaag
    21301 gagcaacgtc actcgatacc catgaagttc agcctatcct gcaagcatat ggcatgaaca
    21361 cgctccccac ctggattgcc agcgatagca ccgaagcggt gcatattgcc gaacagattg
    21421 gttatccggt ggcgctgaaa ttgcgttcgc cggatattcc acataaatcg gaagttcagg
    21481 gcgtcatgct ctacctgcgt acagccaatg aagtccagca agcggcaaac gccattttcg
    21541 atcgcgtaaa aatggcctgg ccgcaggcac gggtccacgg cctgttggtg caaagtatgg
    21601 ctaaccgtgc tggtgctcag gagttgcggg ttgtggttga gcacgatccg gttttcgggc
    21661 cgttgatcat gctgggcgaa ggcggtgtgg agtggcgtcc tgaagatcaa gccgtcgtcg
    21721 cgttaccgcc gctgaacatg aacctggccc gctatctggt tattcagggg atcaaaagta
    21781 aaaagattcg tgcacgcagt gcgctacgcc cattggatgt tgcaggcttg agccagcttc
    21841 tggtgcaggt ttccaacttg attgtcgatt gcccggaaat tcagcgtctg gatattcatc
    21901 ctttgctggc ttctggcagt gaatttaccg cgctggatgt cacgctggat atctcgccgt
    21961 ttgaaggcga taacgagagt cggctggcag tgcgccctta tccgcatcag ctggaagaat
    22021 gggtagaatt gaaaaacggt gaacgctgct tgttccgccc gattttgcca gaagatgagc
    22081 cacaacttca gcaattcatt tcgcgagtca ccaaagaaga tctttattac cgctacttta
    22141 gcgagatcaa cgaatttacc catgaagatt tagccaacat gacacagatc gactacgatc
    22201 gggaaatggc gtttgtagcg gtacgacgta ttgatcaaac ggaagagatc ctcggcgtca
    22261 cgcgtgcgat ttccgatcct gataacatcg atgccgaatt tgctgtgctg gttcgctcgg
    22321 atctcaaagg gttaggctta ggtcgacgct taatggaaaa gttgattacc tatacgcgag
    22381 atcacggact acaacgtctg aatggtatta cgatgccaaa caatcgtggc atggtggcgc
    22441 tagcccgcaa gctcgggttt aacgttgata tccagctcga agaggggatc gttgggctta
    22501 cgctaaatct tgcccaacgc gaggaatcat gagtaaggta ctggaaatgt tgaccactta
    22561 atcgggactg gtgttattat tgcccgctta tgtcgtctgc attgcacaga ggacccttca
    22621 atgaacagag aagaaatgca ctgtgatgtt gtcaaaattt aagcgtaata aacatcaaca
    22681 acaccttgcc caactaccca agatttctca atcagttgat gatgtcgatt tcttttacgc
    22741 tcccgccgac ttccgggaga cgctgctgga aaaaatagcc agcgcgaagc agcgcatttg
    22801 cattgtcgcc ctgtatctcg aacaggatga cggtggcaaa ggcattctga acgcgttgta
    22861 tgaagctaaa aggcagcgtc cggaactgga tgtgcgggtg ctggtcgact ggcatcgtgc
    22921 acaacgtgga cgcattggcg ctgcggcatc taacactaac gctgactggt actgccgcat
    22981 ggcgcaggaa aatccgggcg tagatgttcc ggtttatggc gttccaatca atacccgtga
    23041 agcccttggt gttctgcact ttaaaggctt tatcatcgac gatagcgtac tttatagcgg
    23101 tgccagcctg aacgatgttt acctgcatca gcacgataaa tatcgctacg accgttatca
    23161 tcttatccgt aaccgtaaga tgtcagacat tatgtttgaa tgggttacac agaatattat
    23221 gaatggccgc ggcgttaatc gtctggatga tgttaatcgg ccaaaaagcc cggaaatcaa
    23281 gaacgatatt cgtctgttcc gccaggagct gcgtgatgcc gcttatcatt tccagggcga
    23341 tgccgacaac gatcagcttt ccgtaacgcc gctagtgggg ctggggaaat cgagtctgtt
    23401 gaacaagacc attttccatc ttatgccttg tgccgagcag aaactaacca tctgtacgcc
    23461 atacttcaac ctgccagcaa tccttgtgcg caatattatc cagttgctgc gcgaagggaa
    23521 aaaggtcgaa attattgttg gtgataaaac cgcgaacgac ttctacattc cggaagatga
    23581 acctttcaag ataattggcg cattgcctta tctctatgag atcaatctgc gtcgtttcct
    23641 gagccgtttg cagtattacg tcaatactga ccagctagtg gttcggttat ggaaagatga
    23701 cgacaacacc tatcacctaa aagggatgtg ggttgatgat aagtggatgt tgatcaccgg
    23761 taataacctg aacccgcgcg cctggcgtct ggatctggaa aacgccattt tgatccacga
    23821 tccacaactg gagcttgcgc cgcagcgaga gaaagaactg gagctgatcc gcgagcatac
    23881 caccatcgtt aagcactatc gcgatctgca aagtattgcc gattatccgg tgaaggttcg
    23941 taaactcatc cgccgtttgc gccgtatccg catcgaccga ttaattagcc gcatcctgta
    24001 atcacaaccc cgtcctgtac ggggtttgtt ttttggaggc cacgttttgc gtattctttt
    24061 tgtctgttcg ctattgttac tttctggatg cagccatatg gctaacgata gctggagcgg
    24121 gcaggataaa gctcaacact ttatcgcctc ggcgatgctt tccgccgccg gaaatgaata
    24181 ttcacagcat caggggatga gccgggatcg cagtgccatg tttggattga tgttctctgt
    24241 cagtttgggg gcgtcaaaag agctttggga tagccgcccc gaagggagcg gctggagctg
    24301 gaaggatttg gcctgggatg tcgccggtgc aagcaccggc tataccgtct ggcaactgac
    24361 ccgtcactaa agacgcatcc ccttcccttt gcgatgtagc atcaaagaaa ccagaaacgc
    24421 caccacggcc atcaaggtca cataccagaa gaaggctgtt tccattccta ttgatttcag
    24481 cgacaacgct acgtactccg ccgaaccacc aaatatagca ttagcgaccg catatgacag
    24541 gccaacgcct aatgcgcgaa cctgtgccgg gaacatctca gccttcagta ttccactgat
    24601 tgatgtataa aaactcacta tcagcagggc acacatcacc agaccaaaag cggcataagg
    24661 cgaggaaacg ttttgcaatg ctgagagaat aggaacggta aaaatggctg ccagcgaacc
    24721 gaaacataac attgaggtac ggcgaccaat cttatccgac agcgcgccaa tgagtggttg
    24781 aataagcatg aatacaaaca atgcggcagt cataatgcca ctcgccacgt tggcatgcat
    24841 tcccgcagta tttaccagat acttctgcat ataagtggtg aaggtataga aacaaaggga
    24901 gcccgcagcg gtaaaaccga gaaccatgat gaatgcacgg cgattgcgcc ataatccttt
    24961 cagagatcca gcttctttta aagcgcgcgt ttcttgttgc gaagtttcat ctaattgacg
    25021 acgtaaccac aacgccacaa cagctaacac agctcctaac gcgaaaggaa tacgccatcc
    25081 ccactctctg agtgcagcgt cttccatggt gtgttgtaaa accacgacaa ccagtagggc
    25141 tagcagttgt ccgccgatca acgtcacata ctgaaatgat gcgtaaaaac ctttgcgccc
    25201 ttcaacggca acttcactca tataggtggc gctggtgcca tattctccgc caacggataa
    25261 tccctgaaat aaacgagcga gaagcaataa tgccggagcc cacgtaccta tagtttcata
    25321 acctgggagg caggcgataa ccagcgatcc gaaacacatc atacacaccg ataacagcat
    25381 cgattttttg cgaccatgtt tatcggctat gcggccaaat agccaaccgc ctattgggcg
    25441 catcaggaat cccgcagcaa aaacacctgc tgtttgtagt agttgagtcg tcgtgttccc
    25501 ggaagggaag aaaatgtggg cgaagtagag tgaacagaac gagtagacat agaaatcgaa
    25561 ccactcgacc agattacctg aagaggcccc cacaatcgcc caaatgcggc gacgagtatc
    25621 actacttgtc agtttgctgt ctgccgttac agtactttca gccatgccat tatgtctcct
    25681 gccgtaatcc gatgcttttg tcggtcgctt ttatttattt cttttggtaa agaaaatgtt
    25741 atacatttgt tgcgtatcat tatgcaacct taaccatgaa tttagttagc aggaaaatgg
    25801 ttattgagga gcttaaggat aaatttctgg taaggaggac acgtatggaa gtaggcaagt
    25861 tggggaaacc gcatccgttg ctgaatctgg catatgtggg agtataagac gcgcagcgtc
    25921 gcatcaggca tttttttctg cgccaatgca aaaaggccat cctgacggat ggccttt
//
LOCUS       DAFNBJ010000062        25954 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000062 DAFNBJ010000000
VERSION     DAFNBJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.062"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..454
                     /locus_tag="L2X49_002387"
     CDS             <1..454
                     /locus_tag="L2X49_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBN4797467.1"
                     /translation="SHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAE
                     QKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAA
                     ELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGFS"
     gene            723..2965
                     /gene="nrfB"
                     /locus_tag="L2X49_002388"
                     /pseudo
     CDS             723..2965
                     /gene="nrfB"
                     /locus_tag="L2X49_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            2952..5924
                     /gene="nfrA"
                     /locus_tag="L2X49_002389"
     CDS             2952..5924
                     /gene="nfrA"
                     /locus_tag="L2X49_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBN4797468.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRAQL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVPTRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNTSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5925..6815
                     /locus_tag="L2X49_002390"
     CDS             5925..6815
                     /locus_tag="L2X49_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBN4797469.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6998..7759
                     /gene="envY"
                     /locus_tag="L2X49_002391"
     CDS             6998..7759
                     /gene="envY"
                     /locus_tag="L2X49_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBN4797470.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7805..7881)
                     /locus_tag="L2X49_002392"
     tRNA            complement(7805..7881)
                     /locus_tag="L2X49_002392"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7845..7847),aa:Arg,seq:tct)
     gene            8124..8756
                     /gene="fimZ"
                     /locus_tag="L2X49_002393"
     CDS             8124..8756
                     /gene="fimZ"
                     /locus_tag="L2X49_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HBN4797471.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSKTLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(8759..9274)
                     /gene="sfmF"
                     /locus_tag="L2X49_002394"
     CDS             complement(8759..9274)
                     /gene="sfmF"
                     /locus_tag="L2X49_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HBN4797472.1"
                     /translation="MRRALFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(9285..10292)
                     /gene="fimH"
                     /locus_tag="L2X49_002395"
     CDS             complement(9285..10292)
                     /gene="fimH"
                     /locus_tag="L2X49_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HBN4797473.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(10305..12913)
                     /locus_tag="L2X49_002396"
                     /pseudo
     CDS             complement(10305..12913)
                     /locus_tag="L2X49_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(12944..13636)
                     /gene="fimC"
                     /locus_tag="L2X49_002397"
     CDS             complement(12944..13636)
                     /gene="fimC"
                     /locus_tag="L2X49_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBN4797474.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(13856..14398)
                     /gene="fimA"
                     /locus_tag="L2X49_002398"
     CDS             complement(13856..14398)
                     /gene="fimA"
                     /locus_tag="L2X49_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBN4797475.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            14869..15735
                     /gene="folD"
                     /locus_tag="L2X49_002399"
     CDS             14869..15735
                     /gene="folD"
                     /locus_tag="L2X49_002399"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBN4797476.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            15737..15949
                     /gene="ybcJ"
                     /locus_tag="L2X49_002400"
     CDS             15737..15949
                     /gene="ybcJ"
                     /locus_tag="L2X49_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBN4797477.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            16057..16578
                     /locus_tag="L2X49_002401"
     CDS             16057..16578
                     /locus_tag="L2X49_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBN4797478.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(16614..17999)
                     /gene="cysS"
                     /locus_tag="L2X49_002402"
     CDS             complement(16614..17999)
                     /gene="cysS"
                     /locus_tag="L2X49_002402"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBN4797479.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            18173..18667
                     /gene="ppiB"
                     /locus_tag="L2X49_002403"
     CDS             18173..18667
                     /gene="ppiB"
                     /locus_tag="L2X49_002403"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBN4797480.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            18670..19392
                     /gene="lpxH"
                     /locus_tag="L2X49_002404"
     CDS             18670..19392
                     /gene="lpxH"
                     /locus_tag="L2X49_002404"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBN4797481.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQGLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            19510..20019
                     /gene="purE"
                     /locus_tag="L2X49_002405"
     CDS             19510..20019
                     /gene="purE"
                     /locus_tag="L2X49_002405"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBN4797482.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            20016..21083
                     /gene="purK"
                     /locus_tag="L2X49_002406"
     CDS             20016..21083
                     /gene="purK"
                     /locus_tag="L2X49_002406"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBN4797483.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(21220..22113)
                     /locus_tag="L2X49_002407"
     CDS             complement(21220..22113)
                     /locus_tag="L2X49_002407"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBN4797484.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(22110..22925)
                     /locus_tag="L2X49_002408"
     CDS             complement(22110..22925)
                     /locus_tag="L2X49_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBN4797485.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGTP"
     gene            complement(22936..24195)
                     /locus_tag="L2X49_002409"
     CDS             complement(22936..24195)
                     /locus_tag="L2X49_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBN4797486.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(24205..25872)
                     /locus_tag="L2X49_002410"
     CDS             complement(24205..25872)
                     /locus_tag="L2X49_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBN4797487.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSQDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         6394 a         6861 c         6475 g         6224 t
ORIGIN      
        1 gagtcacggc agagaagaaa tccgtcttca tattgtttgc gatgtccctg atgaacttat
       61 tgatttcacg tttgaatgga aaggtctgaa gaaattatgc gtggcagtct cctttcggtc
      121 aataatagca gaacaaaaga aagagcccga aatgacggtc agatattata tcagttctgc
      181 tgatttaacc gcagagaagt tcgccacagc aatccgaaac cactggcacg tggagaataa
      241 gctgcactgg cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa gaagaggaaa
      301 cgccgcagaa ttattttcag ggatacggca catcgctatt aatattttaa cgaatgataa
      361 ggtattcaag gcagggttaa gacgtaagat gcgaaaagca gccatggaca gaaactatct
      421 cgcgtcagtc cttgcgggga gcgggttttc gtaatcttgc cctgcctgct cacctcctcc
      481 aggcacttcc actacattat gacctgctcg gtgccaccgc ttcttttcac tatgacgtta
      541 acctcccctc cttgacacca tgcttttgtc tacattctac gcaaacggca acttttataa
      601 ccagtgtaaa aataacgtgc cgcaatagtc atacctatta atggtaaaaa gctgtcacaa
      661 ttcataaaaa accttaatat acgccaccct aaacataacc agcgttaatg taaggttttt
      721 gtgtggactg gcttcttgat gtttttgcaa cctggctcta cggcttaaaa gtaatcgcga
      781 taacgttagc ggtcatcatg ttcatcagcg ggctggacga tttttttatt gatgtcgtct
      841 actgggtacg ccgcattaaa cgcaagttga gtgtttatcg ccgctacccg cgaatgagtt
      901 accgcgaact gtataaacca gatgaaaaac cgttagcgat tatggttccg gcgtggaatg
      961 aaacgggcgt catcggcaat atggccgagc tggcggcgac cacgctcgac tacgaaaact
     1021 atcatatctt tgttggcacc taccccaacg accccgatac tcaacgtgat gttgacgaag
     1081 tgtgcgctcg ctttccgaac gtgcataagg tagtctgcgc gcgtcctggt cccaccagca
     1141 aagccgactg tctgaacaac gtgctggacg ccatcaccca gtttgaacgt agcgccaatt
     1201 tcgcttttgc tggttttatt ctgcatgacg ccgaagatgt gatttcaccg atggaattgc
     1261 gtctgttcaa ctatctggtc gagcgtaaag atctgattca gatcccggtg tatccgttcg
     1321 aacgcgaatg gacgcacttc accagcatga cttacattga tgagttttca gagctgcatg
     1381 gcaaagatgt tccggtgcgt gaagccctcg ccggacaagt gcccagccca gcgcaggcgt
     1441 cggcacctgt ttcagccgcc gcgccgtgac cgctctgtta gctgacggtg acggtattgc
     1501 tttcgacgtg cagagtctga ctgaagatta cgacattggt ttccgcctga aagagaaagg
     1561 tatgacggaa atttttgtcc gttttccggt ggtggacgaa gccaaagaac gcgagcagcg
     1621 caaattttta cagcacgcac ggacgtcaaa catgatctgc gtgcgcgaat atttccccga
     1681 taccttttcg actgcagttc gacaaaaatc tcgctggatc atcggcattg ttttccaggg
     1741 ctttaaaacc cacaaatgga cctccagcct gacgctgaac tactttctct ggcgcgaccg
     1801 caaaggggca atcagtaact ttgtcagctt cctcgcgatg ctggtgatgc tccagctttt
     1861 gctgttgctg gcgtatgaaa gtttgtggcc caatgcctgg catttccttt ctatttttag
     1921 cggcagcgca tggttaatga ccctgctgtg gctaaacttt ggcttgatgg ttaaccgcat
     1981 cgtgcagcgg gtgattttcg tcactggcta ctacggtctg acgcaggggc tgctatctgt
     2041 cctgcgtctt ttctggggca acctgattaa cttcatggcc aactggcgcg cgctaaaaca
     2101 ggtacttcaa cacggcgatc cacgtcgcgt cgcgtgggat aaaacaacgc atgacttccc
     2161 cagcgtgact ggcgataccc gctcgttgcg cccgttaggc caaattctgc tggaaaatca
     2221 ggtcatcact gaagaacaac tcgatacagc actgcgtaat cgcgtcgaag gtctacgcct
     2281 gggcggttca atgctgatgc aggggctgat tagcgccgag cagctggcac aggcgctggc
     2341 agagcaaaac ggcgtggcgt gggaatccat cgatgcctgg cagatcccgt cctcgctgat
     2401 tgccgaaatg ccagcctccg tagcgctgca ttatgcagta ctgccgctgc gtctggaaaa
     2461 tgatgagtta attgtcggca gtgaagatgg tattgacccg gtttcgctgg cggccctgac
     2521 gcgtaaagtc ggacgcaaag tgcgttacgt cattgttctg cggggacaaa ttgtcacggg
     2581 gttacgtcac tggtatgcac gccgacgcgg tcacgatccg cgggcaatgt tgtacaatgc
     2641 ggttcagcat cagtggctca cggaacagca gaccggtgaa atctggcggc aatatgtgcc
     2701 gcatcagttc ctgttcgccg aaatactgac cacgctcggt catattaatc gttcagcaat
     2761 taacgtgttg ttattgcgcc atgaacgcag ttctctgccg ctcggcaagt ttttggtcac
     2821 cgaaggcgtt atcagccagg aaacgttgga tcgcgtcctg acaattcaac gcgaattaca
     2881 agtttcgatg caatcactat tactcaaagc aggtttaaac acagaacagg ttgcgcaact
     2941 ggagtccgaa aatgaaggag aataacctta atcgcgtcat cggatggtct ggtttactgc
     3001 tgacgtcttt attgagtacc agcgcactcg cagacaatat cggcaccagc gcagaagagc
     3061 tggggctgag cgattatcgc cattttgtta tttatccccg tctcgataag gcgctgaagg
     3121 cacagaaaaa taacgacgaa gcaaccgcca tccgcgaatt tgaatatata caccagcagg
     3181 tgccggataa tattccgctg actttatacc ttgcggaagc ctatcgccat tttggtcatg
     3241 atgaccgggc gcagctgttg cttgaggatc aactgaaacg tcacccagga gatgcccgac
     3301 ttgagcgcag tctggcggct attccggttg aagtgaaaag cgttacgact gttgaagaac
     3361 tgcttgccca gcaaaaagcg tgcgacgctg cgccgaccct gcgttgtcgc agtgaagtcg
     3421 ggcagaatgc cctgcggctg gcacaattac ctgtcgccag agcgcaactg aacgatgcga
     3481 cgtttgctgc atcgccagaa ggaaaaacgc tgcgaaccga tctgctgcaa cgggcaatct
     3541 acctgaaaca atggtcccag gcagatacgc tatacaatga agcacgccag cagaacacat
     3601 taagcgcggc agaacgccgt cagtggtttg acgtgcttct tgccgggcag ctggacgatc
     3661 ggatcctggc actgcaatca caggggatct tcaccgatcc tcagtcatat attacttacg
     3721 cgaccgcgct ggcttatcgt ggcgaaaaag cacgcctcca gcattatctc aatgaaaata
     3781 agccgctgtt taccacggac gcacaagaga aaagttggct ctatctgtta tctaaataca
     3841 gcgccaaccc cgttcaggca ttggcgaatt atacggtgca gtttgccgat aaccgccagt
     3901 atgttgttgg cgcgacgcta ccggtgctgt taaaagaagg tcagtacgac gcagcgcaaa
     3961 aactgctcgc caccctcccc gccaatgaaa tgcttgagga gcgttatgct gtcagcgtgc
     4021 cgacccgtaa caaggctgaa tctctgcgtc tggcacgatt gctgtatcag caagaaccgg
     4081 caaatcttac ccgcctggat caactgacct ggcaactgat gcagaacgag cagtcacgcg
     4141 aagctgccga tttattgctg caacgctatc ctttccaggg cgatgcgcgt gtcagccaga
     4201 ctttaatggc gcgactggcg tctctgctgg aaagtcatcc ttacctggca acgccggcga
     4261 aggtggcgat tttatcgaaa cccttaccgc tggcggagca acgtcagtgg caaagtcagt
     4321 tgccgggtat tgcagataat tgcccggcaa tagttcgctt gctgggcgat atgtcgcctt
     4381 cctacgatgc tgccgcctgg aaccgtctgg caaagtgtta tcgggacacg ctacccggtg
     4441 tggcgctgta tgcatggctt caggccgaac aacgacaacc gaacgcctgg caacatcgtg
     4501 cggtagccta tcaggcgtat caggttgagg actacgccac cgcactggcg gcctggcaga
     4561 aaatcagtct tcacgacatg agcaatgagg atctgcttgc tgctgccaat accgcccagg
     4621 cggcaggaaa tggtgcagct cgcgatcgct ggctacaaca ggcagaacaa cgtggactgg
     4681 gaaacaatgc cctctactgg tggctgcatg cgcaacgtta cattcctggt cagccggaac
     4741 tcgcactgag cgatctcacg cgctcaatca atattgcgcc ttctgccaac gcttacgttg
     4801 cgcgggcgac aatttatcgc caacgtcata atgtcccggc ggcggtgagt gatttgcgcg
     4861 ccgcgctgga actggaaccg aatactagca acatccaggc agcgcttggt tacgccttgt
     4921 gggatagcgg tgatatcgca cagtcgcggg aaatgctcga acaggcgcat aaagggcttc
     4981 cggacgatcc ggcactgatc cgacaactgg cctatgtgaa ccagcgtctg gatgacatgc
     5041 ctgcgacgca gcattacgcc cggctggtga ttgatgacat tgataatcag gcgctgataa
     5101 ccccactgac cccagagcaa aatcagcaac gcttcaattt ccgccgtttg catgaggagg
     5161 tcggtcgccg ctggacgttc agtttcgatt cttctatcgg cttgcgttcc ggggcaatga
     5221 gcaccgctaa caataatgtc ggcggcgcag cgccagggaa aagctatcgt agctacgggc
     5281 aactggaagc cgagtaccgc atcggacgca atatgctgct ggaaggcgac ctgctctcag
     5341 tttatagccg cgtctttgcc gataccggag aaaacggggt gatgatgccg gtgaaaaatc
     5401 cgatgtccgg caccggtctg cgctggaagc cgctgcgcga tcagatcttt ttcctcgccg
     5461 tcgaacagca gttgccgctg aacgggcaaa atggcgcatc cgataccatg ctgcgcgcca
     5521 gcgcctcatt ctttaatggc ggcaaataca gcgacgaatg gcacccgaac ggttcaggct
     5581 ggtttgccca aaacctgtac ctcgatgcgg cgcaatatat ccgccaggat attcaggcgt
     5641 ggacggcaga ttatcgcgtc agctggcatc agaaggtagc taacggacag actattgagc
     5701 cttacgctca cgttcaggac aacggctatc gtgataaagg cactcagggc gcgcagcttg
     5761 gcggagtcgg ggtccgctgg aatatctgga ccggcgagac gcactacgac gcctggccgc
     5821 acaaagtcag tctcggcgtc gagtatcaac atacctttaa ggcgattaat caacgtaacg
     5881 gagagcgcaa caacgcgttt ctcaccattg gagtgcactg gtaaatgcgt aagttcattt
     5941 tcgtattgct gacactgctt ttggtcagcc ctttttcctt tgcgatgaaa ggtattatct
     6001 ggcaaccaca aaaccgagat agtcaggtta ccgataccca gtggcagggg ctgatgagtc
     6061 agttacgttt gcaaggcttc gataccctgg ttttgcaatg gacccgttac ggcgatgcat
     6121 tcacccagcc agaacagcgc gcgttattgt ttaagcgtgc cgcagctgcg caacaggctg
     6181 gcctgaagct gattgtcggg ctgaacgccg atccggaatt ttttatgcac cagaaacagt
     6241 cgtccgcagc gctggaaagc tatcttaatc gcctgctggc tgccgatctc cagcaagcca
     6301 gattatggag cgccgcgcct ggcgtaacgc cggatggctg gtacatcagc gcggaaattg
     6361 acgacctgaa ctggcgcagc gaagctgccc gtcagccttt gctaacatgg ttaaacaacg
     6421 cgcagcggct gattagcgat gtttcagcaa aaccggttta tatcagtagt tttttcgccg
     6481 gaaacatgtc gcccgatggc tatcgccaac tgctggaaca agttaaagca accggcgtta
     6541 atgtctgggt acaggatggc agcggcgtgg ataaacttac cgctgaacag cgtgaacgtt
     6601 atctacaggc cagcgccgat tgccaaagtc ccgcccctgc cagcggcatt gtttatgaac
     6661 tgtttgtcgc cggcaaaggc aaaaccttta cagcgaaacc gaaaccggat gcagagatcg
     6721 cctcgctgtt agcgaaacgt tcctcttgcg gtaaagacac tctctatttc tctctgcgct
     6781 atttgcccgt cgcgcacggc attctcgagt attaaatctc ctccaggtaa gtcgggtacg
     6841 acctggctta cctctttcgc tctcgtcaga attccatcaa gattaattgc taaaaagcgt
     6901 gctaaagaaa gaagatcgtt atcggcgaac gggccgatca ctcatgcagg aagcatagat
     6961 cctttgagcg gagtttctga tatctgagga gtgcgaaatg caattgagca gcagtgaacc
     7021 ttgcgtggtg atcctgaccg aaaaagaggt agaggtaagc gtcaataacc atgctacgtt
     7081 tacccttccg aaaaactacc tggccgcctt cgcgtgcaac aataacgtca ttgaactctc
     7141 aacgttaaat cacgtattaa tcccccacat caaccgtaac atcatcaacg attatctgtt
     7201 gtttttaaat aagaacttaa cctgtgtaaa gccctggtcg cggctggcaa ccccggttat
     7261 cgcttgtcat agccgtacac cggaagtgtt ccggctagcc gccaaccaca gcaagcagca
     7321 acccagcaaa ccctgcgaag cggagttgac gcgcgcatta ctttttaccg tattgtctaa
     7381 ctttcttgag caatcgcggt ttatagccct actgatgtat atcttacgca gcagcgtccg
     7441 cgacagcgtc tgccgcatta ttcaaagcga tattcagcat tactggaatc tgcgaattgt
     7501 cgccagttcg ctatgtttaa gccccagcct actcaaaaag aaattaaaaa acgaaaatac
     7561 cagctatagc cagattgtca cagagtgtcg tatgcgttac gccgtacaga tgttattgat
     7621 ggataacaaa aatatcactc aggtggcgca attatgtggc tatagcagca cgtcgtactt
     7681 tatctctgtt tttaaggcgt tttacggcct gacaccgttg aattatctcg ccaaacagcg
     7741 acaaaaagtg atgtggtgaa gggcaaagcg gaaacggata agacgggcat aaatgaagaa
     7801 gaaatggcgc gccctgcagg attcgaacct gcggcccacg acttagaagg tcgttgctct
     7861 atccaactga gctaagggcg cgttgatacc gcaatgcggt gtaatcgcgt gaattatacg
     7921 gtcaaccctt gctgagtcaa tggcttttga tctggttgct gaacaagtga acgaccgcgt
     7981 ctgattttct gatttatttc gctatagcgg caaacaaaca cacaccgctg cgcgtctgaa
     8041 tcaagaaaac ccgtattttc atgtatcaaa gtacaattta ccgccctaac ggaaaattgt
     8101 ccgctcctat gagactggta actatgaaac caacgtcggt gatcattatg gatactcatc
     8161 ctatcatcag aatgtctatt gaagttctgt tgcaaaaaaa cagtgaattg cagattgtcc
     8221 tgaaaacgga tgattaccgc ataaccatcg attatctccg aacccgtcct gttgatttaa
     8281 tcattatgga tatagacttg cccggaacag acggttttac cttcctgaaa aggatcaaac
     8341 aaatccagag cacagtgaaa gtgttatttt tatcatcgaa atcagaatgc ttttatgctg
     8401 gcagagcgat acaagctggt gctaacggtt ttgtcagtaa atgcaatgat cagaatgata
     8461 tttttcatgc cgttcagatg atcctctccg gatacacgtt ttttcccagc aaaacgctta
     8521 actatataaa aagcaataaa tgtagtacga atagttcaac gatcactgtg ctatctaatc
     8581 gtgaagtgac catattacgt tatctggtta gcggattatc taataaagaa attgccgata
     8641 agttattact tagcaataaa acagttagtg cgcataaatc taatatttat ggcaagctag
     8701 gtttgcattc aattgtagag cttatcgact acgccaaatt atacgaatta atataatatt
     8761 aattataatt gatcataaat atcgcatccg ctttcgccac acctggccga acaccgctgg
     8821 ctaacgctcg ataattggca aaaaagttta gtgtgacatt gccatttgca tctacttcct
     8881 cagtcgggct cgcctccccc agtgcgagcc gggagcgatc gctattacgt aattcgatgg
     8941 cgacggtttg tgccattgcg ggatcatcca gagccagcag gttggtatcg gatgccggcg
     9001 ttcccgtaaa taaaatcgca actgaacccg gaggacatcc ctccagccgc aggctaaaag
     9061 ggacgagtgc cgtggtatcg ccagcgttca gtaattgtgt cgtaggccat ctgcctaaat
     9121 ctaccgtctt atcaatatcc gcagtgttta cggtacagga gaaatcaaca acgttaccgt
     9181 gcaaattgat attaatcgtt cccaaagggt cagctgccca tccactggaa ctccacagta
     9241 acccgcagaa acagctaaag agtgctcttc tcattctccg tacctcattg ataatcgacg
     9301 cgtaaatacc ccagcgcgct aaacggccct tcggtcggtt tttgaccggt aatactgata
     9361 ggccaggcgc gaagtgtgac attggctgcc gcagctgcat ccagacggaa aggaataacg
     9421 ctattgagat cgttaggcgt gatcggcgta tcgttctgat cggcgacaat aaaacctaaa
     9481 tcctgattgt ccgacaccat cgcctgacca gaaacggcac tggcttccag acgcattgtt
     9541 aaataagcct gcgcagcaac attcgtacat ttgaccgcaa tgctcttggt ttgcggcatg
     9601 acaccagcag gtcgattacc cggccctgcc gcactaaata acgatgcgcc gatatcacca
     9661 aaatcaaatt caacaatctg cccggcattt aattcgcagt tttgcggtac ttcaacccgg
     9721 ccaccaaaac taatggtata aacaggtgta accaacggat cgtaaggcgt tgatgtcaca
     9781 tacacggtaa acatcacctg tctggggatc tccaccatgt taataaaggg acgaataacc
     9841 tttaaacgaa atattaaacg agaatccatc accccgaatg gaaggttttg cgaaacgtta
     9901 ggatctgttc ccatcagaat ataattttgc ggggggaact gaatatccat cacactatca
     9961 accagactca tcgcacctaa tagataatca tttaattgca tatatttata attatcgata
    10021 gtttcctgaa caataaagtt ggtgacataa ctacggtatg tataattcac cagcgtaccg
    10081 ggtggacaaa ttgctgagac acctacccag ccggattttt ccggtaaaac aacaatctgc
    10141 cctggctggt tattggtgct attaaagata tttgagagat catagtgttc atcttctgct
    10201 acgccagtac tatttgcaca aacagtagcc caggatatgt ttgccagaca caaacatgcc
    10261 atcgccaata ataatctgca gattatcttc attgataatt tcctttatga gccaggatgt
    10321 gtgcaaactg ccgaaataac agtgacggct tgctgtaagc tttgcttcgg caatacatag
    10381 tgcgcaatgc agcgtgagtt cgcgccgtct ccccactgaa ccagtaattc accagacaat
    10441 ggcgcaccac ttaaataaac ttgcccgtca tcacccacat actggttatt ccgatactgt
    10501 tttcccgtac cagtgatcca aacggcaccg gttttccgcc ctgggtaacg gtaatgagcg
    10561 cccgcacgcc tatgcgggta tcaaaattgg cacgaaccaa cgcgccttgc gtcggcacaa
    10621 cgctgctaat atttttttca acatcgatgg aattccccat cgtattggta tcaagcgcga
    10681 tacggttata ccgataaacc gtggcatacg gcatcaccgc atagccgcgc cagtcggtta
    10741 aaatgccagt ttgattttca atgcgtacac cgcctgcgcc aggcgcttta atcaaaacat
    10801 tggtatcccc aaaaggctgg ctcagcgtta tgccattttc atgcccgacc acaccgccag
    10861 acagctgcca gttaacgtca tgttgatcgc ggtcgtagtt atacccgacc cccagcgtag
    10921 cgtaagcggc ctgccagttt gccgtggcgc tgcccgtata cccattattc gaggtatcac
    10981 cctggcttac gtgataactc aggttgtggc cttccagtaa ggtcccacct acacctgcca
    11041 gccagctatt ttgcccgttg ctgttacggt tggcgttaaa ggaggcataa gcgcggtcga
    11101 gcgcattttc ccgggtgtag cgacgtttgg tcagaacatt gaaaggcact gaaacattaa
    11161 gtccgacaat acgttcgtta tcggggatcc ctacagattc attccacgaa aatgagagtg
    11221 aataactgat gccaacccag ctgctggtat accccacctg ataccaggta tctgaatccg
    11281 aagtattcca gtatttttga tgggtaccag agatataaag cgagccaaag tcattaagtg
    11341 attgtgaaat atttaactgc aaacggtctt tacggctaaa gcgtaaattg tggtaattca
    11401 cgattatcgg ttcatcgcga tgctcaccgt cgtaatcgta atcatattcg tacccctcca
    11461 ttcgacgata cgcaacatca tcaagggtat aaaaaccttg tgtcgaatag cggtaaccca
    11521 ttaactgaaa attggtgccg aaggtgttca tcgatttcgc atagaggaag cgaatggaat
    11581 ccccctcatg acgactgtcg tcggctaact gactgcgcgc atgggtcaca tccagcgaca
    11641 ctgcccccca gttcccgagg ttgcgcccca gccctaataa aaaggcggtg taattggcag
    11701 ataattgcgt cccgccgtag gcagtaaatt cctgtggtaa accgccgagt gccgtgccct
    11761 gaaaaaagaa aggcgatgat tgctgactat taccgctacg aaaatcgccc gccgtcaggt
    11821 caaatttgaa acgcccttcg cgttgtaaaa ttggcactgt tgaatacggg atagtgtaat
    11881 tctgctgatt accatcgcgc tcgtcgatgg tgacgtccag atcgccattt gaagatgtcg
    11941 ggtgcaaatc tgtaatttca aaagcgccgg gggaaacata gctttgatag ataataaaac
    12001 cattttgccg aatcgttagc tgggccgccg tacgggcaat cccacgtacc gttggggcaa
    12061 acccttgctg gctatcagga tacatattat cggaagaata aagccgtaca ccacgaaatc
    12121 caacaccatc gaaaatatca cttcctgtat tgccgtctcc cattaccagt tcacttttca
    12181 gcggaataat ggcacgctgt acccaggtgc caatattatt ccactgttct gaatgatatc
    12241 catttccgcg aaaatagttc caggaaccat tgttgcgtaa acgccacggg ccaatattaa
    12301 tcccgctgaa ctcactaaaa aaatagctat cattaccatt acctctgtta ccggtgaaat
    12361 tataattcag gagtaaagca ggaatacctt catcccactc ttcaggcgga atgtaaccgt
    12421 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
    12481 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
    12541 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
    12601 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
    12661 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcgcg
    12721 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
    12781 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
    12841 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
    12901 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
    12961 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
    13021 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
    13081 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
    13141 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
    13201 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
    13261 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
    13321 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaag gtattttcgc
    13381 ttttgggttc actaacaaac agtggcggtg taatgatgaa tgacttttct tttacaccgc
    13441 tgctgttttc aatccacgaa ttgaccagaa agcgctcatt ggtatggcta tttctaatcc
    13501 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
    13561 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tataatgagc atcaataact
    13621 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggaata
    13681 taaagaaatg attgctttgg tttcttacaa cacatcattc ccttttcatt taataaagcg
    13741 gcatgataca aagtgaacat gatttcgatt tacattgcca ccataccgct tcagacaacc
    13801 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
    13861 catatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
    13921 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
    13981 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
    14041 cgacacccgt tgctgttgtt gtattggtac tggatgcaat ggccaataaa ttatcattga
    14101 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
    14161 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
    14221 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
    14281 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
    14341 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
    14401 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
    14461 gacactgata gtagtaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
    14521 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcata atatattcaa
    14581 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
    14641 tatttcttaa attatcttaa tccttagaca aggaaataaa tcagttccag atttacaacg
    14701 ccatcatgga cgaaaaatga agctttcagt ctcagcgacg gtgcgcctca ccttcgcaag
    14761 aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc ttctgacaaa
    14821 ataggcgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat ggcagcaaag
    14881 attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca aaaagttcag
    14941 gcgcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt gggtagtaac
    15001 cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt cgggttcgtc
    15061 tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga gcttatcgat
    15121 acgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt accggcgggt
    15181 attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga cggtttccat
    15241 ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg caccccgcgc
    15301 ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa cgccgtggtg
    15361 attggcgcat cgaatatcgt tggccgcccg atgagcatgg aactgctgct ggcaggttgc
    15421 accactacag tgactcaccg cttcactaaa aatctgcgtc atcacgtaga aaatgccgat
    15481 ctattgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat caaagaaggc
    15541 gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gtaaagttgt gggcgacgtc
    15601 gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg cggcgttggc
    15661 ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga atatcatgat
    15721 ccacaggatg agtaacatgg cgacattttc tttaggtaaa catccgcacg ttgagctgtg
    15781 cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa tcgcgattgc
    15841 cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca aaatcgtcgc
    15901 cggtcagaca gtgagttttg caggtcacag cgtacaggtt gttgcctgat ccctttcccg
    15961 gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc cgttcatcca
    16021 tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat tacgcacgcc
    16081 gccgttcccc tatgcattgg tttagggttg ggttcgaaag tcatccctcc gcgcctgtta
    16141 tttgccggaa tcatcctggc gatgctgccc gatgccgacg tcttgtcgtt taattttggc
    16201 gttgcttacg gcaatgtttt tggtcatcgc gggtttaccc attcgctggt gtttgcgttt
    16261 gttgtcccgc tgttatgtgt gctgattgga cgtcgatggt tcagggctgg gctgattcgc
    16321 tgctggctgt ttttaaccgt ctcgttgcta tcgcacagct tgctggattc ggtaaccact
    16381 ggcggtaaag gggttggctg gctgtggccg tggtcagatg aacgcttttt cgctccctgg
    16441 caggtgatta aagtcgcgcc gtttgcattg tctcgttaca ccacagcgta cgggcatcag
    16501 gtgattattt ctgaattgat gtgggtgtgg ttgccgggga tgctactgat gggaatgttg
    16561 tggtggcgca gacgataacc ggatgcgaaa actcgcatcc ggcaatagcg caattactta
    16621 cgacgccagg tggtcccttg cgggccatct tccagcacga tccccatctc attaagacgg
    16681 tcacgcgccg catctgccgc cgcccagtct ttcgctttac gggcatccag acgctgttga
    16741 attaacgctt caatctcagc cacttcgctg tcgtctgcct gcgcgccgct ttgcaggaac
    16801 gcttccggtt cttgctccag caggcccaat acggcggaaa gtttacgcag gtgagatgcc
    16861 attgcattcg ccgctgccat atcttctgct ttcagacggt ttacttcacg cgccatatca
    16921 aacagcacgg aataggcttc cggggtgttg aaatcgtcgt ccatcgcttc aataaagcgc
    16981 gcttcaaacg cttcgccacc ggcaggcgca acggttttat ctgtgccgcg cagcgcagtg
    17041 tagagacgct ccagcgccgc acgcgcctgc ttcaggttct cttcgctata gttcagctgg
    17101 ctgcgatagt ggcccgacat caggaagtaa cgcacggttt ccgcgtcgta gtatttcagc
    17161 acatcgcgca cggtaaagaa gttacccagc gatttggaca tcttctcgcg gtcaaccatc
    17221 accatccccg agtgcatcca gtagttcaca tactgaccat catgggcaca ggtggactgc
    17281 gcgatttcgt tttcgtggtg cgggaacatc aggtctgaac cgccgccgtg gatatcaaag
    17341 tggttaccca gctgcttgca gttcattgcc gaacattcaa tgtgccagcc aggacgaccc
    17401 gcgccccacg gagacggcca gctcggttcg ccctctttcg acatcttcca cagaacgaag
    17461 tccattgggt tgcgtttgtc gtcgaccacg tcaacgcgcg cgcctgcctg cagctggtcg
    17521 agatcctgac gcgacagcac gccataagtt ggatcggtcg ggacgtcgaa catcacgtcg
    17581 ccgttgtccg ccacataagc gtgacctttg gcgatcagtt gttcagtgag ttcaataatt
    17641 tctgcgatat ggtgcgtcgc gcgcggctcc atatccgggc gcagaatgtt caacgcatca
    17701 aaatctttgt gcatttcggc gatcatgcga tccaccagcg ccacaaagct ttcgccattt
    17761 tcattggcgc gtttgatgat tttgtcgtcg atatcggtaa tgttgcgcac atacttcagc
    17821 ttatagccga ggaaacgcag atagcgcgca accacgtcaa aagcaacaaa ggtacgcccg
    17881 tgaccgatat gacagagatc gtaaacggtg attccacaca cgtacatgcc gacttccccg
    17941 gcgtgaatag gcttaaattc ctctttttgg cgtgtcagag tattgaagat ttttagcatc
    18001 gaagattccg tttagacatg tgtgggtaat tgagttgcgt ataataccca tatttcccgc
    18061 gcgaatcagc atacattgcg tgatgatccg atcgtgcggt tatgctataa caccacccta
    18121 tatatgaccc gaactgggtt gaagcaccaa tcaaacggaa caggatgcaa aaatggttac
    18181 tttccacacc aatcacggcg atattgtcat caaaactttt gacgataaag cacctgaaac
    18241 agttaaaaac ttcctggact actgccgcga aggtttttac aacaacacca ttttccaccg
    18301 tgttatcaac ggctttatga ttcagggcgg cggttttgaa ccgggcatga aacaaaaagc
    18361 caccaaagaa ccgatcaaaa acgaagccaa caacggcctg aaaaataccc gtggtacgct
    18421 ggcaatggca cgtactcagg ctccgcactc tgcaactgca cagttcttca tcaacgtggt
    18481 tgataacgac ttcctgaact tctctggcga aagcctgcaa ggttggggct actgcgtgtt
    18541 tgctgaagtg gttgacggca tggacgtggt agacaaaatc aaaggtgttg caaccggtcg
    18601 tagcggtatg caccaggacg tgccaaaaga agacgttatc attgaaagcg tgaccgttag
    18661 cgagtaatcg tggcgacact ctttattgca gatcttcatc tctgcgtgga agaaccggcg
    18721 atcaccgccg gttttctgcg ttttttagcg ggggaagccc gcaaggccga cgcgctgtat
    18781 attcttggcg atctgtttga agcatggatt ggcgacgacg atcccaaccc actccatcgc
    18841 cagatggcgg cagcgatcaa agcggtgtcc gattccggcg ttccctgtta tttcattcat
    18901 ggcaaccgtg attttctgct cggcaaacgc tttgcccgtg aaagcggcat gacattattg
    18961 ccggaagaaa aggtgctcga actttatggt cgccgggtgt tgattatgca tggcgacacg
    19021 ctgtgcaccg atgacgcggg ttatcaggct tttcgcgcca aagtccacaa accctggctg
    19081 caaatgctat tcctcgctct gccgttgttt gtgcgcaaac gcattgccgc gcgaatgcgc
    19141 gcgaacagta aagaagccaa tagcagtaaa tcgctggcga tcatggacgt taaccaaaac
    19201 gcggtagtca gtgcgatgga aaaacatcag gtgcaagggc tgatccacgg gcatacccat
    19261 cgcccggcgg tgcatgaact tatcgccaat cagcaacctg cttttcgcgt ggtactgggt
    19321 gcctggcata cggaaggttc gatggtgaaa gtcacggcgg atgacgttga gctaattcat
    19381 tttccgtttt aaaaaacccg caactttgct gatttcacag ccacgcaacc gttttccttg
    19441 ctctctttcc gtgctattct ctgtgccctc taaagccgag agttgtgcac cacaggagtt
    19501 ttaagacgca tgtcttcccg caataatccg gcgcgtgtcg ccatcgtgat ggggtccaaa
    19561 agcgactggg ctaccatgca gttcgccgcc gaaatcttcg aaatcctgaa tgtcccgcac
    19621 cacgttgaag tggtttctgc tcaccgcacc cccgataaac tgttcagctt cgccgaaagc
    19681 gccgaagaga acggttatca ggtgattatt gcgggcgcag gcggcgcagc gcatctgcca
    19741 ggcatgattg ccgccaaaac gctggtgccg gtgctgggcg tgccagtaca gagcgccgca
    19801 ctgagcggtg tcgatagcct ctactccatc gtacaaatgc cgcgcggcat tccggtgggt
    19861 acgctggcga ttggtaaagc tggcgcggca aacgcggcgt tactggcagc acaaattctt
    19921 gcgactcatg ataaagaact gcaccagcgt ctgaatgact ggcgcaaagc ccagaccgac
    19981 gaagtgctgg aaaacccgga cccgcgaggt gcggcatgaa acaggtttgc gtcctcggta
    20041 acgggcagtt aggccgtatg ctgcgtcagg caggcgaacc gttaggcatt gctgtctggc
    20101 cagtcgggct ggacgctgaa ccggcggcgg tgccttttca acaaagcgtg attaccgctg
    20161 agatagaacg ctggccggaa accgcattaa cccgcgagct ggcgcgccat ccggcctttg
    20221 tgaaccgcga tgtgttcccg attattgctg accgtctgac tcagaagcag cttttcgata
    20281 agctccacct gccgactgca ccgtggcagt tacttgccga acgcagcgag tggcctgcgg
    20341 tgtttgatcg tttaggtgag ctggcgattg ttaagcgtcg cactggtggt tatgacggtc
    20401 gcggtcaatg gcgtttacgc gcaaatgaaa ccgaacagtt accggcagag tgttacggcg
    20461 aatgtattgt cgagcagggc attaacttct ctggtgaagt gtcgctggtt ggcgcgcgcg
    20521 gctttgatgg cagcaccgtg ttttatccgc tgacgcataa cctgcatcag gacggtattt
    20581 tgcgcaccag cgtcgctttt ccgcaggcca acgcacagca gcaggcgcaa gccgaagaga
    20641 tgctgtcggc gattatgcag gagctgggct atgtgggcgt gatggcgatg gagtgttttg
    20701 tcaccccgca aggtctgctg atcaacgaac tggctccgcg tgtgcataac agcggtcact
    20761 ggacacaaaa cggtgccagc atcagccagt ttgagctgca tctgcgggcg attaccgatc
    20821 tgccgttacc gcaaccagta gtgaatagtc cgtcggtgat gatcaatctg attggtagcg
    20881 atgtgaatta tgactggctg aaattgccgc tggtgcatct gcactggtac gacaaagaag
    20941 tccgtccggg gcgtaaagtg gggcatctga atttgaccga cagcgacaca tcgcgtctgt
    21001 ccgcgacgct ggaagcctta atcccgctgc tgccgccgga atatgccagc ggcgtgatgt
    21061 gggcacagag taagttcagt taacctggtg ctcatttgat tgccggatgc ggcgtaaacg
    21121 ccttatccgg cttaccgctc cggtacccat tgtaggcctg ataagacgcg tcaagcgtcg
    21181 catcaggcat ggtgcaccga ttgccggatg ctctcatcac tacagcgaaa tacaggtccc
    21241 cgcttcgccc gccagcgtct cttcaattcg cgataacgcc ccaatccacg cgggtttacc
    21301 acggcttctg acataaccac ttaccgccgt taccttcggc cccatcgaac catcggcttt
    21361 ggcaaatggc gctaactcat ccggtgtggc atggcgaatg gcgcgttgct gcggcgttcc
    21421 ccagttttca taaaccgcgt cagcatcggt gaggatcacc agtccatcgg cattaatctg
    21481 ctcggcgagc aacgcagcgg cgagatcttt atcaatcacc gcttcactcc ctgccccatc
    21541 ttccgtcaca ggcacaccgc cgccgccact gcaaatcacc acatgcccct ctttgagcaa
    21601 caactcgatg gcttcgctgt cgagaatttt acgcggttgc ggagacgcca ccacccggcg
    21661 caaatattta ccatcacgtt tcatctgcca gccgtaagcc gcttccagtg ccttttgttc
    21721 ttctggctga taaaccggac caataaattt ctctggctgc aaaaacgccg gatcatcagg
    21781 cgaaacctca atgcgcgtca gcaccgtcgt gacaggcggc atctgcggct gtgcgctcaa
    21841 actctgcgcc agcatatagc caatcatccc ctggctttcc gccaccagca catccagcgg
    21901 atacggatct acctctttcc acgccagatt ctgcaatgcc agcagcccca cctgcggccc
    21961 attgccgtga acaatcgcca gccgataaga acgggccagg cgcgccagcg cgggtacagc
    22021 actggcgata ttgcgatatt gattttctgc cgtcagcgcc tcaccgcgct ggagtaaggc
    22081 gttgccccca agagctacaa ccagtgtttt catggtgttc cttgtaataa ttgttgacca
    22141 agccagaacc ccagcagcgt atctgcccct gacgtgtgcc ccagcgccag cagcgaatca
    22201 atcgcaacag cggtacgttt cgggcaactc agagcatgaa caaagtgcag gagtggcgag
    22261 gcgaaatatc cttgcgcggc gtagcgtaaa taactgacgc tcaccgctgt ggtaacgagt
    22321 tgaagattgt cggagcaggc aaaaaactgg cgaccggagc gcgcatctaa agcgccataa
    22381 taccaggccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt taatccgggg
    22441 cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc ctgaaaacag
    22501 tgacgaaact ggcgtaattc agcggtcagc ggatcgcttg ccgccatcgc cagtggtccg
    22561 aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg aggatgtgaa
    22621 ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa acgcccgagg
    22681 cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc gcataacgca
    22741 tcgaattgcg cgcggcgaag cacccatcct ccggggccga aaccactacc ctgacgatgc
    22801 agcgttaaca gttcgccgct ttccgtcatc aggttaatcg cccgtcgcca cactcccgct
    22861 aaccgccagg actggcgata attcggtgca ctacggctgg caagcagagg atggatgatc
    22921 gtcattgcgc gttcctcaac caatccccat gctttcagcc agtgcttcca gcgcctgttc
    22981 aaaacacgcc agcggtgccc gcacagtgcc tgcgccaatc tgcccgatcc ccgcctcttt
    23041 atgggcgata ccggtattga tgagtggcgt aatgccggtt tctaccacgc gacgaatgtc
    23101 cagtcccagg cacgcgccct gaaaatccca gcctgggatc tgcaactgca tattgcgctc
    23161 aaggtaaatt tccgccatct cttcagatac cgctctggcc gcttccatgc cacccgcacc
    23221 gacaaagcgc gttacgccag gcgctgcgat cattgccgca cctccgatac caaaggtttc
    23281 ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc cggtgaaaaa
    23341 cagaccttgc ggagtgttta caggcgcagt aaaccagcgt tcgcccagcc cgcttacccg
    23401 aatcccgaac atattgccgt tgcgggtcat tgccgtgacg atgctgcctg cgcggatcat
    23461 cgcgccagca tccatcgccg ccttgcagta agccatcgcg aggttgagga agaactgatc
    23521 ggtcacgctg aggaaatcca tcacttcggc gatgtgctgt ttatcatgat cgaggcgagc
    23581 aatttgtggg gccagcgcac gcattaacag tgcagaggaa gcaatattgc gttgatggaa
    23641 ctcatcgccc atcgtaatgc cctgcgccat catcgccgtg agatcgatac cgcgctccat
    23701 gcgccccagc gccgcgctta atactggcat taacacatcg cgcatccagc gatggcgagt
    23761 caggacatct tcgccgtaag cgccaaaacg catcactttg ccgatacctt cgttgaggtt
    23821 gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc agcatcggca tactggcaga
    23881 agtaataccg cccattggac cgacggcatt cacatggtga caaggaatga agttcacttc
    23941 cccctgctcc agtattgcca gcgcctgcgc ttcatctttc gcccagcctt cgaacagaca
    24001 tgcgcccacg cacgcccctt tcatgggtcc ggtcatctcc tgccagcgca ttggcggccc
    24061 ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata agtgaagaag ccggttgcac
    24121 atccagccag tgtggacgag cacgacgaat ttgttcgata accgcagcat tggcttgcgc
    24181 cactgatgta aacatgggaa ccccttattg caaacgttct aataaacgag ccagtttttt
    24241 attgccgccg gcgactggcg accattggta atgcacaacc ggtttgctgg cgctttgtag
    24301 ctccagcgca aagctgcgta atccgatgtt aatcacggcg acgttttcca gtaatgacgg
    24361 tgtgtgttgc tgagtggcag gcgaaagcgg acgaatcagt gccgccgcca gcaaggtggc
    24421 ttccggcagc gaactcacta ccgcgatccc tgcatcttcc agcgtggcga tttgctgcga
    24481 gcggcattgc gggtcacgtt cagtgcctgt caccgtggca atggcataca gtggttgatt
    24541 gtctgaacgc gcggcacatg ctttttgcca ggcgcttacc agcgaggcgg caggatcggc
    24601 ggtcgcaccg aagccaatca cgacatcaag cagcaatacg cgcacttgcg gtttagcgcc
    24661 gagatcggca attaactgat tgcgtaaggc tgggtcgatc atcggatggg gacgcccgac
    24721 ggtgtagaaa tcatcgccga ggtcaataat ctggtgacca tcggcgtcga gcatcatgcc
    24781 gtgatgatgg gtatcgtcgg cttccacgcc aaggtgcccg gcaagtaatc ccgccgcttc
    24841 ggcagccagc gtaccgccgg tatacaaacc gcaaataaat ccgctgctgg caggcgttat
    24901 cgcgttacgt cgcgccgtaa cgcgtgaaag caggcaagcc aggcgtgcgg cctcatcctg
    24961 cgaggaggca aaccagacat tctcgtcgcg ggccaccgcc ggggtataac ctaaaaacag
    25021 cgccaccgtc ggtttgccgg ttgctttcat ggcattaaca attttcagac gcacagcttc
    25081 ggcaggcggt tttgaaacaa atgccagcac ttcgcttttc tcgtctgcac tgagcatttc
    25141 cagcgccgtc agcgcactga tgccgcccac ttcacggctg aggtcgcgcc cgccaaggcc
    25201 aatcgcgtga gtaattccct cccctgccag cgcaatctgt gagcacagct cctgaatccc
    25261 ggtaccggaa gcgccaatga cgccaatgtt gccttccggc atcacgttag caaaagccag
    25321 cggcgtggcg gcaatcatcg acgtaccgca gtccggcccc atcaccagca agcctttttc
    25381 tcgcgcgcgg gttttaagtt ggatttcatc ttccagcgtg acgttatcgg agaacatcat
    25441 cacgttgagg ttgcgatcca gcgcctgatt cgccagctcc gccgcatact cgccagccac
    25501 tgaaatcagc gccagattgg catcggataa tttctgacag gcactgtccc agcgacgcac
    25561 ctgcgtcaag gactggctgc tgcctgaccc ctgcgccagt tgttttagcg cctcttcaag
    25621 ctgctgcata atcgcctgcg cgatccccgc atccgccgct tcgctacgta ttgccacgca
    25681 aatatcgttc ggcgtggcgt tattaaaatc gtcatgccag aaacctgtgg tatctaataa
    25741 cgctttattg gcgggcgtac ccatcattac ggaaacatca tcaacatttt ctgattcgct
    25801 gagttttcgt gaaataatca ttaaactgac cgaatcctga aaacaccctt ttttaataaa
    25861 ggcgtggatc ataacaactc cttaaaaatt gcacccatgg caattgcacg agcagtgtta
    25921 atgtggcgat tacggtatcg atgacaacga gtaa
//
LOCUS       DAFNBJ010000063        25874 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000063 DAFNBJ010000000
VERSION     DAFNBJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.063"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            86..487
                     /locus_tag="L2X49_002411"
     CDS             86..487
                     /locus_tag="L2X49_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HBN4797488.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            506..1204
                     /locus_tag="L2X49_002412"
     CDS             506..1204
                     /locus_tag="L2X49_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HBN4797489.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1255..1914
                     /locus_tag="L2X49_002413"
     CDS             1255..1914
                     /locus_tag="L2X49_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HBN4797490.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            1932..2330
                     /locus_tag="L2X49_002414"
     CDS             1932..2330
                     /locus_tag="L2X49_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBN4797491.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2340..2978
                     /locus_tag="L2X49_002415"
     CDS             2340..2978
                     /locus_tag="L2X49_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBN4797492.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAIFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVISGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2981..4144
                     /locus_tag="L2X49_002416"
     CDS             2981..4144
                     /locus_tag="L2X49_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBN4797493.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4228..5853
                     /locus_tag="L2X49_002417"
     CDS             4228..5853
                     /locus_tag="L2X49_002417"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBN4797494.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQGDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5970..6245)
                     /gene="yjfN"
                     /locus_tag="L2X49_002418"
     CDS             complement(5970..6245)
                     /gene="yjfN"
                     /locus_tag="L2X49_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBN4797495.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6394..6723)
                     /gene="bsmA"
                     /locus_tag="L2X49_002419"
     CDS             complement(6394..6723)
                     /gene="bsmA"
                     /locus_tag="L2X49_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBN4797496.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6905..7654
                     /gene="yjfP"
                     /locus_tag="L2X49_002420"
     CDS             6905..7654
                     /gene="yjfP"
                     /locus_tag="L2X49_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBN4797497.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7651..8406)
                     /gene="ulaR"
                     /locus_tag="L2X49_002421"
     CDS             complement(7651..8406)
                     /gene="ulaR"
                     /locus_tag="L2X49_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBN4797498.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8514..9578)
                     /gene="ulaG"
                     /locus_tag="L2X49_002422"
     CDS             complement(8514..9578)
                     /gene="ulaG"
                     /locus_tag="L2X49_002422"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBN4797499.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQVDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9933..11330
                     /gene="ulaA"
                     /locus_tag="L2X49_002423"
     CDS             9933..11330
                     /gene="ulaA"
                     /locus_tag="L2X49_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBN4797500.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11346..11651
                     /gene="ulaB"
                     /locus_tag="L2X49_002424"
     CDS             11346..11651
                     /gene="ulaB"
                     /locus_tag="L2X49_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBN4797501.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            11661..12125
                     /gene="ulaC"
                     /locus_tag="L2X49_002425"
     CDS             11661..12125
                     /gene="ulaC"
                     /locus_tag="L2X49_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBN4797502.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQDVLDLIDRTNAAA"
     gene            12139..12789
                     /gene="ulaD"
                     /locus_tag="L2X49_002426"
     CDS             12139..12789
                     /gene="ulaD"
                     /locus_tag="L2X49_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBN4797503.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12799..13653
                     /gene="ulaE"
                     /locus_tag="L2X49_002427"
     CDS             12799..13653
                     /gene="ulaE"
                     /locus_tag="L2X49_002427"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBN4797504.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13653..14339
                     /gene="ulaF"
                     /locus_tag="L2X49_002428"
     CDS             13653..14339
                     /gene="ulaF"
                     /locus_tag="L2X49_002428"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBN4797505.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14468..14743)
                     /locus_tag="L2X49_002429"
     CDS             complement(14468..14743)
                     /locus_tag="L2X49_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBN4797506.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            15071..15466
                     /gene="rpsF"
                     /locus_tag="L2X49_002430"
     CDS             15071..15466
                     /gene="rpsF"
                     /locus_tag="L2X49_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBN4797507.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDADAGDSEE"
     gene            15473..15787
                     /gene="priB"
                     /locus_tag="L2X49_002431"
     CDS             15473..15787
                     /gene="priB"
                     /locus_tag="L2X49_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBN4797508.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15792..16019
                     /gene="rpsR"
                     /locus_tag="L2X49_002432"
     CDS             15792..16019
                     /gene="rpsR"
                     /locus_tag="L2X49_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBN4797509.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            16061..16510
                     /gene="rplI"
                     /locus_tag="L2X49_002433"
     CDS             16061..16510
                     /gene="rplI"
                     /locus_tag="L2X49_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBN4797510.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(16622..17593)
                     /locus_tag="L2X49_002434"
     CDS             complement(16622..17593)
                     /locus_tag="L2X49_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBN4797511.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSQENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(17646..18947)
                     /locus_tag="L2X49_002435"
     CDS             complement(17646..18947)
                     /locus_tag="L2X49_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBN4797512.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            complement(18990..19466)
                     /locus_tag="L2X49_002436"
     CDS             complement(18990..19466)
                     /locus_tag="L2X49_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBN4797513.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            complement(19487..21568)
                     /locus_tag="L2X49_002437"
     CDS             complement(19487..21568)
                     /locus_tag="L2X49_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBN4797514.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            complement(21565..23106)
                     /locus_tag="L2X49_002438"
     CDS             complement(21565..23106)
                     /locus_tag="L2X49_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HBN4797515.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDELVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDITPDGLHIAQSGQIPKLVTKVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(23116..23892)
                     /locus_tag="L2X49_002439"
     CDS             complement(23116..23892)
                     /locus_tag="L2X49_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBN4797516.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMVNQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            complement(23902..24744)
                     /locus_tag="L2X49_002440"
     CDS             complement(23902..24744)
                     /locus_tag="L2X49_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBN4797517.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(24754..25545)
                     /locus_tag="L2X49_002441"
     CDS             complement(24754..25545)
                     /locus_tag="L2X49_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBN4797518.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNLWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            25758..>25874
                     /locus_tag="L2X49_002442"
     CDS             25758..>25874
                     /locus_tag="L2X49_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN4797519.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKA"
BASE COUNT         6376 a         6381 c         6654 g         6463 t
ORIGIN      
        1 ttttttgtct atagctggta agataattac gtactgcaaa tattcatttt attaatattt
       61 aaactaaatc tatatggaat aatatatgac atggaatccg ttggcgctgg cgacggcgct
      121 gcaaactgta cctgaacaaa atattgatgt gacaaatagc gagagcgcat taattattaa
      181 aatgaatgat tatggcgatt tgcaaatcaa tattcttttt acttcccgcc aaatgattat
      241 cgaaaccttt atttgtccgg taagtagtat cagcaatcca gatgaattta ataccttctt
      301 attaagaaat cagaagatga tgccgttgtc atcggtagga atctccagcg tgcaacagga
      361 agagtattac attgttttcg gggcgttatc gcttaaatct tctcttgaag atatcctgct
      421 tgagataacc tcgctggtag ataacgcatt ggatctggct gaaattacag aagaatattc
      481 acactaatta caaggacggg tatttatggg aattttaaaa agtttattta cgctggggaa
      541 atcgtttatc tcccaggcgg aagaatccat tgaagaaacc cagggcgtgc gcatgctgga
      601 acagcatatt cgtgacgcta aagctgaact cgataaagcc ggaaaatctc gcgttgatct
      661 gctggcgcgg gtgaaattaa gtcacgataa gctgaaagat ttacgtgagc gcaaagccag
      721 tctggaagcc cgtgcgctgg aagcgttaag caagaacgtt aatccgtcgt tgattaacga
      781 agttgctgaa gaaatcgcgc gccttgagaa tctcattacc gctgaagagc aagtgttgtc
      841 gaatctggaa gtctcccgtg atggcgtgga aaaagcagtt acagcgacag cgcagcgtat
      901 cgctcagttt gagcagcaaa tggaagtcgt taaagccact gaagccatgc agcgtgcaca
      961 acaggctgta acaacctcta ccgttggcgc atcttccagc gtttcgacag cggcagaatc
     1021 cttaaaacgc ctgcaaacgc gtcaggccga acgtcaggct cgcctggatg ctgccgcaca
     1081 gttggagaaa gtcgcagacg gtcgcgacct tgacgaaaag ctggcggaag ccggaattgg
     1141 cggtagcaat aaaagtagcg cccaggatgt attagcaaga ctgcaacgcc aacagggcga
     1201 gtaatttttt tgccagcctc gctaaaaggc tggcaactat tttaaggata aaatatgtct
     1261 ggttttttcc agcgtctgtt tggcaaggat aataagccag ctatcgctcg tggtccgctg
     1321 ggacttcatc tcaatagtgg ctttacgctc gacacgttag cgtttcgttt gctggaagat
     1381 gaattgctga ttgcgctgcc gggtgaggaa tttacggtag ccgccgtcag ccacatcgat
     1441 ctgggcggcg gtagtcagat tttccgctac tacacttcag gcgatgaatt tctgcaaatc
     1501 aataccaccg gcggcgaaga tattgatgac attgatgata tcaagctctt tgtctatgaa
     1561 gagagctacg gtatcagtaa agagagtcac tggcgcgagg cgatcaacgc caaagtgatg
     1621 ggggcaatga tcttaaactg gcaggaaaaa cgctggcagc gattttttaa cagcgaagaa
     1681 ccgggaaata tcgaacccgt ctacatgctg gaaaaagtag aaaatcaaaa ccatgccaaa
     1741 tgggaggtcc ataattttac catgggctac caacgccaag taaccgaaga tacttacgaa
     1801 tatttgctgt taaatggtga ggaatctttt aacgatctcg gcgagccaga gtggttattt
     1861 tcgcgcgcgt tgggtgtcga tatcccgctg acatcacttc atattattgg ttaattacaa
     1921 ggacgtttac aatgcatata ctggattcac ttcttgcctt tagtgcctat ttttttattg
     1981 gcgtggctat ggtgattatt tttctgttta tctactccaa aattacaccg cacaacgaat
     2041 ggcaattaat caaaaacaat aataccgcgg cgtcactggc attcagcggt acattgttgg
     2101 gttacgtgat ccccttatcc agtgcggcaa tcaatgcggt gagtattcca gactatttcg
     2161 cctggggcgg gattgcactg gtgattcagt tactcgtttt tgctggtgtc agactttata
     2221 tgcccgcatt aagcgaaaaa attattaatc acaataccgc agcaggaatg tttatgggaa
     2281 ccgccgcact ggctggtggt atttttaacg cagcttgtat gacatggtaa tggacgatca
     2341 tggccagaaa acgtaaatca agaaataaca gtaaaattgg tcacggagcg atcagtcgca
     2401 ttggaagacc gcataatcct tttgagccgc gccgcaatcg ctacgcacaa aaatacttaa
     2461 cgttggtgct aatgggcggt gccatttttt taatagtcaa aggatggagt gaaagtagcg
     2521 atgtcgataa cgacggcgac ggaacgtttt actcgacagt gcaggattgt attgatgacg
     2581 gtaataatgc cgacatttgc gcgcgtggct ggaataacgc caaagcggcg ttttatgccg
     2641 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtatgaaaat tgttactacg
     2701 acaacgttga acagagttgg atcccggtaa tatctggatt tttgttaagc cgggttattc
     2761 gtaaagatcg cgatgagcag tttgtttata acagcggcgg atcctcattt gcttcccgcc
     2821 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
     2881 cttactcttc gggcggcttt accaccaaaa aagcgtcaac cgtttctcgc ggcggctatg
     2941 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctaagac acaacgttcc
     3001 tgtgcggcgg gatctggacc agatcgccgc tgacaacggt ttcgactttc atatcatcga
     3061 caatgaaatc tattgggatg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
     3121 gcagatcgaa aaaccgactg cggaactgca tcagatgtgc cttgaggtgg tggatcgagc
     3181 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
     3241 cgccgaaagc tggcacgcac gcgatccttc gctgtatggc cggatggatt ttgcctggtg
     3301 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgacct cgttgtatga
     3361 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     3421 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     3481 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     3541 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     3601 agatctcggt ttgggcgtcg gcggcgtact gaccgatctt gatgataatg tcatccagcg
     3661 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     3721 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     3781 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     3841 aggtgaaaaa ccgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     3901 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     3961 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccac gatttggcga
     4021 tagctacaca ctcatcggta gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     4081 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     4141 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtgacc atactaatgg
     4201 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     4261 cctataccat taaataacag taacttatac ctgtctgatg gcgcgctttg cgaagcggta
     4321 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     4381 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgctgcgc
     4441 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     4501 atgcaggcgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     4561 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     4621 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     4681 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     4741 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggcggttcc
     4801 gatgtcatga gcaacaccac ccgcgcagag cgcctggaag atggctctta tcggctggtg
     4861 gggcataaat ggtttttctc ggtgccgcaa ggcgatgcgc atctggtgct ggcgcagact
     4921 acgggcggtt tgtcctgctt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     4981 attcgcctcg agcggctgaa agataagctg ggtaatcgct ctaacgccag ctgcgaagtg
     5041 gagtttcagg atgccattgg ctggttgttg gggcaggaag gggaaggaat tcgtctgatc
     5101 ctgaaaatgg gtgggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     5161 cgtgcatttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     5221 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     5281 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gtgccgatgc caaagaagcc
     5341 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaaag aggtatgccg
     5401 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     5461 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     5521 tgcctggatg tgctgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     5581 gcgtttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     5641 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     5701 ctgggctgcg gtgcgcaaat gttgaaatat gcctctccgc caatggcgca ggcgtggtgt
     5761 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     5821 ttgctgcggg cgacgggggg agtgtgtgtg taagcgtata cgactgatgc gacgctggtt
     5881 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     5941 cggcattgtt catttgctgt agatgacact cacgcataca gtatcgcctg tacacgccag
     6001 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     6061 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     6121 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     6181 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     6241 ttcatcgtca gctccatttt tcatttcccc acatgttgca gggcattcca tcgcggtaat
     6301 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     6361 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     6421 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     6481 tgcagcgaca gctttcgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     6541 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     6601 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     6661 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     6721 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     6781 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     6841 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     6901 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     6961 tgtcggacaa aaagataccc cgttaccgtg cgcgattttt tatcacggct ttacttcgtc
     7021 cagtctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     7081 gccggatgcg cccgatcacg gtagtcgttt tagtggcgac gcagcgcggc ggttaaatca
     7141 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     7201 cgaagaaaag tggctgcttg atgaccgtct ggcagtcggt ggtgcgtcga tgggcgcgat
     7261 gacggcactg gggattaccg ctcgccaccc cacggtgaga tgtaccgcca gcatgatggg
     7321 atcgggctat tttacatcac tcgcccgttc actgtttcca ccgctgatac ctgaaacgac
     7381 agcacagcag aatgaattca ataacattgt cgcgccgctg gcagagtggg aagcgacaaa
     7441 ccacctggaa caacttagtg acagacctct actgctgtgg catggcctcg acgatgacgt
     7501 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     7561 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     7621 tgctgcagtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     7681 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgatcagca tatcaatttg
     7741 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     7801 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgccgtca gcatatcggt
     7861 tttatacaac ccttctgctg tcagtccttt gccgctggta aacatccagt gcccggcata
     7921 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     7981 gcccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     8041 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     8101 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcgat
     8161 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     8221 ctgttgggta atagcttctg cgccattgcg cacttttttc agtttgccgc tttcgtcaag
     8281 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     8341 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     8401 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagccaggaa
     8461 atactacgcc ggatagcgcg aactactcca cgctatccgg tataaacgac agattacagg
     8521 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc acgcggatag
     8581 tggtactcga agttgtcttt atccaacggc caggtaaatt tgccgccaac ctgccagata
     8641 aacggcttga agccatactt caggcgatct tttttcatct cccacagcac gcggatctct
     8701 tgcggatcgg cctggaagtt tgaccagata tcgtggtgga acgggataac cactttcgca
     8761 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt gataccgcgc
     8821 gggttttcgc cgtatgaacc taaagccacg tcgatctgat gttcgttacc gtgcttcgca
     8881 taatagttag agtagtggga gtcgccgctg tgatacaggg agccgccagg cgttttgaac
     8941 agatagttca ccgcgcgatc gtccatgccg tccggcagta cgccagccgc tttttgatcg
     9001 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     9061 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     9121 ccaatccaca aatccacaca ggttttcggt ccaatgaacg gtacgtcatc agcacagttc
     9181 tgcatcacag cagcagcgac gttgacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     9241 accgcgtcga cctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgcaggttt
     9301 ggctgcagtt ttttcacgcc agccatgcgc tgcatttggt gaccctgttt cattaacggg
     9361 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     9421 tccgatttta accagatccc ggtgcagcca agccaccaca tcgcaaatgt gccaggagcg
     9481 acctgttctt gttcaatttc ttcattcaac cagctacccc actccgggaa agtgctcagg
     9541 atccaggatt cacgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     9601 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     9661 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     9721 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     9781 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     9841 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     9901 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     9961 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
    10021 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
    10081 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
    10141 tggcgaaaat gtccgaagtc tacggcatta acggcgcaat ttccgatact tacgcgtcga
    10201 tgatggcaac catcgaccgc atgggcgatg cctatagctg ggtaggttac gcggtgttgt
    10261 tagcgctggc gctgaacatc tgttacgtgc tgctgcgtcg cattaccggt attcgtacca
    10321 tcatgctgac tggtcatatc atgttccagc aggcggggct gattgccgtt acgttgttta
    10381 ttttcggcta ctccatgtgg acaaccatta tctgcacggc gattctggtt tcgctctact
    10441 ggggcattac ctccaacatg atgtacaagc cgactcagga agtgacggac ggctgcggtt
    10501 tctccatcgg tcaccagcag cagtttgcat catggattgc ctataaagtt gcgccgttcc
    10561 tcggcaaaaa agaggaaagc gttgaagacc tcaaactgcc aggctggctg aatattttcc
    10621 acgacaacat cgtctccacg gcgattgtca tgaccatctt ctttggtgcc attctgctct
    10681 ccttcggtat cgacaccgtg caggcgatgg caggcaaagt gaactggacg gtgtacatcc
    10741 tgcaaactgg tttctccttt gcggtggcga tcttcatcat cactcagggc gtgcgcatgt
    10801 ttgtggcgga actctctgaa gcatttaacg gcatttccca gcgcctgatc ccaggtgcgg
    10861 ttctggcgat tgactgtgcg gctatttata gcttcgcgcc gaacgccgtg gtctggggct
    10921 ttatgtgggg cactatcggt cagctgattg cggttggcat cctggtcgcc tgcggttcct
    10981 cgatcctgat tattcctggc tttatcccga tgttcttctc caacgccacc atcggcgtgt
    11041 tcgctaacca cttcggcggc tggcgtgcgg cgctgaagat ttgtctggtg atggggatga
    11101 tcgaaatctt tggttgcgtc tgggcggtga aacttaccgg tatgagtgcc tggatgggta
    11161 tggcggactg gtcgattctg gcaccgccga tgatgcaggg cttcttctcc atcggtatcg
    11221 cctttatggc cgtcatcatt gtaattgcac tggcttatat gttcttcgct ggccgcgcac
    11281 tgcgcgcaga agaagatgca gaaaaacaac tggcagaaca gtctgcttaa taaggagttt
    11341 tgattatgac cgtacgtatt ctggctgtgt gtggcaacgg acaaggcagt tccatgatca
    11401 tgaagatgaa agtggaccag tttttaaccc aatcaaacat tgaccacacg gtaaacagct
    11461 gcgcggttgg cgaatacaaa agcgagttga gcggcgcgga tatcatcatc gcttctacgc
    11521 acattgcggg cgaaatcacc gtgaccggca acaaatacgt ggttggtgtg cgcaacatgc
    11581 tctctcctgc cgactttggc ccgaaactgc tggaagtgat caaagagcat ttcccgcagg
    11641 atgtgaagta aggacgcgcc atgaaattac gtgattcgct ggcggagaat aaatccatcc
    11701 gcctgcaggc tgaagctgag acatggcagg acgcagtaaa aatcggcgtt gacctgctgg
    11761 tggcggcgga tgttgtcgag ccgcgttact accaggcgat tctggatgcg gttgaacagc
    11821 acggtccgta ctttgtgctc gcaccaggcc tggcaatgcc gcacggtcgc ccggaagagg
    11881 gcgttaagaa aaccggtttt gcgctggtga cgctgaaaaa gcctctggag ttcaaccacg
    11941 aagataacga cccggtggat atcctcatca ccatggcggc ggtcgatgcc aacacgcacc
    12001 aggaagtggg catcatgcag atcgtcaacc tgtttgaaga tgaagaaaat ttcgaccgtt
    12061 tacgtgcctg ccgtaccgag caggatgtac tggatctcat tgaccgcacc aacgcggcag
    12121 cttaagaagg aattgaagat gtcattaccg atgttacaag tcgcgctgga caaccagact
    12181 atggatagcg cctacgaaac cactcgcctg attgccgaag aagtcgacat tatcgaagtg
    12241 ggtaccattc tgtgcgtggg cgaaggcgtg cgtgcggttc gcgatctgaa agcgctttac
    12301 ccgcacaaaa tcgtactggc agacgccaaa attgccgatg cgggcaaaat cctctctcgt
    12361 atgtgcttcg aagccaacgc tgactgggtg acggtaattt gctgtgcgga tatcaacacc
    12421 gccaaaggcg cgctggacgt agcaaaagag tttaacggcg acgtgcagat cgaactgacc
    12481 ggctactgga cctgggaaca ggcgcagcag tggcgtgacg caggcattca gcaggtggtt
    12541 tatcaccgca gccgtgacgc gcaggccgca ggcgtagcgt ggggcgaagc ggacattacc
    12601 gcgatcaaac gtctttccga tatgggcttc aaagtcaccg tcaccggtgg cctggcgctg
    12661 gaagatctgc cgctgttcaa aggtattccg attcatgtgt ttatcgctgg tcgcagcatc
    12721 cgtgatgccg cttctccggt tgaagctgca cgtcagttca aacgttccat cgccgaactg
    12781 tggggctaag gagcggatat gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg
    12841 ctccccgccg gggagtgctg gctggaacgc ctgcaactgg caaaaacgtt gggcttcgat
    12901 tttgtcgaga tgtcggtaga tgaaacggac gagcgcctgt cgcgcctcga ctggagccgc
    12961 gagcagcgtc tggcgctggt gaacgcgatt gttgaaaccg gcgtgcgcgt gccgtccatg
    13021 tgcctttctg ctcatcgtcg tttcccgctg ggcagtgaag atgacgcggt gcgggcgcag
    13081 gggctggaga ttatgcgtaa agctatccag ttcgcccagg atgtcggtat tcgcgtgatc
    13141 caactggcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc
    13201 cgtgacggcc tgaaagagag cgttgagatg gcaagccgcg cgcaggtgac gctggcgatg
    13261 gagatcatgg attacccatt gatgaactcc atcagcaagg cgctgggcta cgcgcactat
    13321 ctcaacaacc cgtggttcca gctctacccg gatatcggca acctctcggc gtgggacaac
    13381 gacgtgcaga tggagttgca ggccggaatc gggcatatcg tcgcggtaca tgtgaaagac
    13441 accaaaccgg gcgtcttcaa aaacgtgccg tttggcgaag gcgtggtgga tttcgaacgt
    13501 tgtttcgaaa cgctcaaaca gagtggctat tgcgggcctt acctgattga gatgtggagc
    13561 gaaacggcgg aagacccggc ggcagaagtg gcgaaagcgc gtgattgggt gaaagcgcgc
    13621 atggcgaaag cgggcatggt ggaggcggca taatgcaaaa gctaaaacag caggtatttg
    13681 aagccaacat ggagctgccg cgctacgggc tggtgacctt tacctggggc aacgtcagcg
    13741 ctatcgaccg cgaacgcggg ctggtggtga tcaagcccag cggcgttgcc tacgaaacca
    13801 tgaaagcggc cgatatggtg gtggttgata tgagcggcaa ggtggtggaa ggggagtatc
    13861 gcccatcttc cgacaccgcg acgcatctcg aactctaccg tcgttacccg tcgcttggcg
    13921 gcattgtcca tacccactcc actcatgcca ccgcatgggc gcaggcgggg ctggcgatcc
    13981 cggcgttagg caccacgcac gctgactact tctttggtga tatcccgtgc acgcgcggat
    14041 taagcgaaga agaggtgcag ggtgagtacg aactgaacac tggcaaagtg attatcgaaa
    14101 cgctgggcaa cgccgagccg ctgcatacgc cgggaattgt ggtgtatcag cacggaccgt
    14161 tcgcctgggg gaaagatgcc cacgacgcgg tgcataacgc ggtagtgatg gaagaagtgg
    14221 cgaaaatggc gtggattgcg cgcagtatta atccacaact caatcacatc gacagcttcc
    14281 tgatgaataa acacttcatg cgtaaacacg gtcctaacgc ttattacggg cagaagtaga
    14341 acacgcgctg cggaaatttc cttcctcggg agataactgg tctaattccg cagccgtttt
    14401 tcaaaaaaag ccccctgaga agggggcaaa gcaaactatg gcaatgtttc tttggttata
    14461 cctggtgcta gcgataaata tccgcgctgg cgtgcatatt gccgttactc cccggttccc
    14521 gcatcagaat cacgtggtag tacgttgcgc cctgcgcatc tgtctcttca tttaatgcct
    14581 gacgcgcttc gctttcagtg gcgaaattat gattgatata aatcacgcct aagctttgca
    14641 catcgtccat atttctggcc tggtggttat taatttcaat ggctgcccat gtatttgcac
    14701 ttagcaaaag cacagccaga agggctaaaa cacggctgaa catagatacc tcctcgacgg
    14761 ctgtttttgt gtgctctcct catgtggtga tggtctgatt taattttaat cacggatgaa
    14821 aaagttgatg gcgaccattt ctgatgcagt tgttcaaaaa aacagcatga tgaagtgtga
    14881 tgaacttcaa atcagcggct tagtggttat ttgcgaaagg ggagatttat ttctgctctg
    14941 cccttgagtt tagcaaggta tgtcagtact ataacgcgtc atttttcagc cgacctttaa
    15001 cacgttcctt gcctccccgg gattcggctg acccagacag gaggctgaat aatccgtaag
    15061 gagcaattcg atgcgtcatt acgaaatcgt ttttatggtc catcctgacc agagcgaaca
    15121 ggttccgggc atgatcgagc gctacactgc tgccatcact ggtgcagaag gcaagatcca
    15181 ccgtctggaa gactggggcc gccgtcagct ggcttacccg atcaacaaac tgcacaaagc
    15241 acactacgtt ctgatgaacg ttgaagctcc gcaggaagtg atcgatgagc tggaaactac
    15301 cttccgcttc aacgatgccg ttattcgcag catggttatg cgtaccaagc acgctgttac
    15361 cgaagcatct ccgatggtta aagcgaaaga cgagcgccgc gagcgtcgcg atgatttcgc
    15421 aaacgaaacc gcagatgatg cagatgctgg ggattctgaa gagtaatttc tgatgaccaa
    15481 ccgtctggtg ttgtccggca ccgtgtgcag gactcccctt cgaaaggtca gtccatcagg
    15541 aattcctcac tgccagttcg tgcttgagca tcgttctgtg caggaggaag ccggttttca
    15601 ccggcaggcg tggtgccaaa tgcccgttat tgttagcgga cacgaaaacc aggccattac
    15661 tcacagtata acggtcggca gtcgcataac cgttcagggg ttcatttcat gccacaaggc
    15721 aaagaacgga ctgagcaaaa tggttttgca tgccgagcag attgaattga tagattctgg
    15781 agactagcca tatggcacgt tatttccgtc gtcgcaagtt ctgccgtttc accgcggaag
    15841 gcgttcaaga gatcgactat aaagatatcg ctacgctgaa aaactacatc accgaaagcg
    15901 gtaagattgt cccaagccgt atcaccggta cccgtgcaaa ataccagcgt cagctggctc
    15961 gcgctatcaa acgcgctcgc tacctgtccc tgctgccgta cactgatcgc catcagtaat
    16021 cggtcacggt ccattaatac gactttgaga ggataaggta atgcaagtta ttctgcttga
    16081 taaagtagca aacctgggta gcctgggtga tcaggtaaac gttaaagcgg gctatgctcg
    16141 taacttcctg gtaccgcagg gtaaagctgt tccagctacc aagaaaaaca ttgaattctt
    16201 cgaagcacgt cgcgctgaac tggaagctaa actggctgaa gttctggcag ctgctaatgc
    16261 tcgcgctgag aaaatcaatg cactggaaac tgttaccatc gcgtctaaag ctggcgacga
    16321 aggtaaactg ttcggttcca tcggtactcg cgacatcgct gacgctgtaa ctgcagctgg
    16381 cgttgaagtg gctaagagcg aagttcgtct gccgaacggc gttctgcgta ccactggcga
    16441 acacgaagtg agcttccagg ttcacagcga agtattcgcg aaagtgatcg taaacgtagt
    16501 agctgaataa ttcgttattc aacgagacgt aaaaacgccg accattggtc ggcgttttgc
    16561 ttttgtagcg agcacttgca gatatcgata agggccacaa taagcagccc tacgaagata
    16621 attacttagc tgcctgaata tcagccagca ttggggcaag ggctgggttg tccttagcga
    16681 agtttgcgta cattggctgt actttctcga taaacggagc tttatctacc tggtagactt
    16741 tcacgcccat tttatccagt tttgcaaatt cttgtttctc ttcctcgctc cacaagtctt
    16801 tcatgtagct cattgattct ttagctgctt ttaatataac tgcctgattt tcctggctaa
    16861 gtttatcaaa cgcagcattg ctcatcagaa ccacatcggg aaccatagtg tgttcgtcaa
    16921 ggctgaagga ttttgccact tcaccgtgac gcatggtagt cagcgccatc acgctgtttt
    16981 ctgccatatc gaccacaccc tgctgtaagg ctgtatacaa ctcgccgtaa tccagtggcg
    17041 tcgggttacc gcccatgact ttgatcattt caactgcgcc agggcttggc tggacacgga
    17101 ctttcatgcc tttcagatcg tctggtttca gtactggttt attgccatag aaactgcggg
    17161 cgcctccatc ataaaaagtc agcccgaaat aacctttgct tttcgttgag tcgaggattt
    17221 ttctcccgat atcgccctgc atgacctgat aataatgatc acgatcgcgg aacaaataag
    17281 gcaggctaaa taatttatag gaattttcaa atgcttccat ttctgccgca ttggttttta
    17341 ccaggggaat agcgccagaa cgaatcagct ccattgtttc tcgttgagta cccaatgttc
    17401 catttggata aatacgaata gtaatatcac cgttagagta ctctttgctc tttttcgcaa
    17461 agaactccat cgctttatga acaggatgag acttatcctg attatgactc agttttaatg
    17521 tcactgccca ggactggctt gcggataaca taagaacagc accgataatg ccagcggata
    17581 cttttatttt catagatgtt ccttttctaa taaatatggc ggcaggtgcc gccagtgaat
    17641 gcgcatcagt agcccagtgc tcttggcaat gccatactga tttgcggaat ataggtcacc
    17701 attgccagca ccaataccat cacggcatag aatggcagca taggtttgat aattttatta
    17761 atcggtaatt tagatacact acaaccgaca aataacacac tgcctacagg cggagtacaa
    17821 ataccgatac acatgttcag taccatgaca ataccaaagt gaatcgggtc catgcccagt
    17881 tcggtgacaa ccggtaaaaa tatcggcgta aaaatcagta ccgccggtgt catatccata
    17941 aaagtgccga taatcaacag gataatattg ataataagca ggattattat tgggttgtca
    18001 gaaatagtgt taagcaaatc gagaatcagg aagggaacgt cagcgtttga cattgcccat
    18061 gacataccca tcgaagaacc aattaataac aagacaattg cagtggtaat aacggattcc
    18121 agaaatattt taggtaaatc tttaactgag atttcacgat aaagaaccag tgcaaggaaa
    18181 agggtataaa ttactgcaat tgcggaggcc tctgtcgggg taaaaatacc acttaataca
    18241 ccccccataa ttaaaataat caggaaaata ctggggatag ccatccatat cgcaacgcca
    18301 gcctggcgta aagtcgggcg ttctggcttt ggataaccgc gacgtaccgc gataataccg
    18361 gctattacca tcagtgccag acccagcaaa attcccggaa cataacccgc caggaacagt
    18421 gcggcaatag aagtaccacc actgaccagt gaatagacga ttaacgtatt acttggtgga
    18481 ataagcaaac ccgtcggggc agaggcaacg ttaaccgcag tactgaatgc cgggtcgtaa
    18541 ccctctttaa cctgttgcgg atgcatgacg ccgcccatcg cagccgccga agccacggct
    18601 gaaccagaaa ttgccccgaa cagcatattg gcgagaatat tacagtgggc aagtgagcct
    18661 ggcagacgac cgccgagaat acgggcaaag ttgattaatc gtcgcgctat tcctccatgg
    18721 ttcataatgt tgccagacaa gataaaaaat gggattgcca acaacgtaaa actatctaac
    18781 ccggaggcta acttttggga aaccacactc aatgctgagt tgaaatcgag ttgtgtggaa
    18841 attgatgcca gtgtcgataa accaatggca taaacaatag gtacacctaa tgccaacata
    18901 aaaaagaagg caccaaataa cacaataatc ggtaaccagt aatccataat ttctccattc
    18961 ctactatggt tggttgttgt ttgttatctt tagagtggtt gtttaagttc ggttaaaacc
    19021 atatctatgg tgtatataat aataagtaca ccactaatcg ggatgctcat atagatatat
    19081 cccatttgcc agcctaaagc gggcgaggtc tgacctgaat cgaatgtatc catcgttaat
    19141 agcccaccac catataccat aatgatggtt gcgaagataa taacgataat ctgaatacac
    19201 aaaaataaat gacgtcgggc ttttcctttc agcatcatag gtagcaggtc aatcgccaga
    19261 tgccggtttt gccctgcggt gtatgcccca ccaaaaagtc caagacaaat aaagagcaaa
    19321 cggctgagtt catctgtaaa aatactggga ctagtaaata taaatcgtgc agccacctgc
    19381 cacgtcacac atgccacgat gatcgccaaa atggtaatgt tcaatgctgc aagcagagca
    19441 ttaatagatc taattatatt tcgcatagtg attaaccttt gcaatatcat tcatcctgtg
    19501 gctggtaaat tgtcccatta agaaaaccag gccagttttt ttcaaaccaa atacctcgga
    19561 taataggaaa ctcacgaaca ggtgtttctc tgacgtgaaa gataccgttg caataatgaa
    19621 taagtagttt atctttttta tcctcagtac gctctgaacg aaagcgccag gcatcacaaa
    19681 aaccatttcg tgtttgcgga atagaatttc catccctgtc gagaataata cgtgctcccc
    19741 gactaccacc acctgcgtca atataatgcg ttaattgcgt taatacggcg gcagaagtca
    19801 gtgccatatg ccgccacata aacagctcgg caacttcacc cacatgtttg atagccaatc
    19861 catgccgttg gacaaattcg ctcaatagca gggcatcgcg agtggcgcgt cggactttat
    19921 cggcatggca aataaatccc gcatggtcag acattcgtgc ctgaattttt tctctcacaa
    19981 ccgacaacgg catcccggtc ccgttatcgt gcgcctgagt aatgatctct cttatagaag
    20041 ccagtgtagg agctaccagc tgtgctatat ctccatcaat attccgcttt tgcgtgcaac
    20101 caataaaacg tgccagacga acagcaaaaa cctgcccggc attcaatgct gcaccaccag
    20161 ggcgagtgac gccgtgtgtg ccagcaactt cccccacggc gaaacagccg ggcagggagg
    20221 tttgtcccca gatatccact tcaatgccgc cattcatatg ctgattattc atggcaaact
    20281 gcaaaggctg tgtggtgaga tcgtaaccgt gcatcttata cagcgagata gacagcggat
    20341 tcattcgttg cagccgttcg atgggcgatg gtgccagagc gtcgttattt tccagatacg
    20401 cgcgaacgtc gtcgtccagt cgatctaatg agaacggcag gtcacccggc accgcttcag
    20461 gattgcgatt gaaatccata aatacctgac gccctgattg ctgctcttgc gctactgcca
    20521 tatctaacaa gctggagcca aaatccatca cccgagtggc gtggaacggc cactgatagc
    20581 ctttacggaa aatgtttgaa gccagctcct gggtggtgcg atagtaatcc gcgaggaagt
    20641 tatactcgtt accctcagca tccacggaat agatatacgg gatcacctgt acataggtgc
    20701 cggataaatt ccacggaaac gtgctacgcg gcgtgcctat accaaactgg ctttcggtca
    20761 gattggttag cgtcaggcct tcctccagcg ccagccccag cgagccaaaa catttgtgtg
    20821 ggtacacact gtcgcgataa agctcgccag gccctcctgt tgccagtacc acattgggcg
    20881 cagtcacaat tgccagcccc caagggttat gggcgcgatg accggtcgcg aggattgccc
    20941 ccgcaacacg gtcttcgccg ttttcgtcac gctgatgcag cagtttaatc actgttgcac
    21001 tggtcaacac tggaatggcg aggcgctgta cttcttccaa cagcactttc accattaacc
    21061 ttgaggtacg cggcccacac gaggttgcac gcccggcttc gtcatggtcg gtttgataac
    21121 gaagaatcgc gccatagcga tcttccggta attccagacc aagatattgc aacccgccaa
    21181 gagtgtgcag agaacctacc gcttcgacat aagcggtgtc gtgatccatc gccccaccgc
    21241 tcgccagtgc ctctgccagt ttggtgaagt tgtcgccgtt gcccgcggta gcggcggtaa
    21301 acagtgtctg tttatcggaa ccagaacatg ccgacgtccc catatataac ccggcggtgg
    21361 cgatcagcac attttgctgg cggcgtttaa gttccacagc tgcacgcagc ccggctgcac
    21421 cactgccgac taccagggca tctgtttgcc agagtgggta atcaacaccg tctaccgtga
    21481 caacccatgc gggctctgcc tgtgcaaaac cctttgggaa agtaacgtgc gtcagcgcat
    21541 caaggatggt gtcatcaagc tgactcatgc atcctccatc ggcgtaaaca ggctactttc
    21601 catcaggcgt gggtgcttga taattggacg gaaagccata tacggcagaa tgtcgcgctc
    21661 aatctccaca cccggcgcga tttcaattaa ttcaacgcct tctgccgtta actggaatac
    21721 tgcgcgttca gtgatataca acacttcctg accactttgc tgtgcgtagg cggcgctaaa
    21781 agtgattttt tccaccttgg taaccagttt agggatttga cctgactggg cgatatgcaa
    21841 gccatctggt gttatatcga tcttgctgcc tttagcgtcg aacgtgccgc agaacaccac
    21901 tttacgggca ttttgcgcaa tttcgagaaa tccgccgggg ccaatcagat tgccattgag
    21961 atgggagacg ttgacgttac cgtactgatc catctctccc atgccgagga aggtgatatc
    22021 gatccctccg ccactgtaga actcaaattg atcgagtgat ggaataatgg catcggcgtt
    22081 gcgggcgcag ccgaaaaacg catcatccag catcatgccg ttatgtacgc cttgctcgac
    22141 gctcagccat aaactttggt aaggaacgcc ttcgcgcagc gctactcctg gaatgccgcc
    22201 gggaatacca aagccaaagt tggttgaagc gccagctacc agttcgcgtg ctgcccgacg
    22261 ggcaatcagg cgacgtaccg gatgactaac cagttcaata gcgtcgttag aactcagacg
    22321 gcgatgttgg ccgctgatgg tcaggtcgta accgccaaga taggtttgtg gctgttcgcg
    22381 gtgttcaacg atgccatcaa ccagaatgcc cggtaattta acccggcgtg gttcaatcgc
    22441 gccagcttcc agcacatcgc gtacctgtac gaacactttg ccgccgctgt tgtgtgctgc
    22501 cagcgccatg gaatagctat ccatatcaat cgcttcttct tcaaggctga cgttgcctcg
    22561 aggatcggca taagtgccac gcaaaatcgc gtaatccact ttgaaagggc gataacgaag
    22621 tttggtttca ccgtcgattt caatcagttc taccagctcg tcggtagtgc attcattcga
    22681 cttaccgccg ccattgcgtg gatcaacaaa cgatcccagc ccaacgtggg taaaaagccc
    22741 cggacgtccg gcgccgatct cccgtagcag tgcctgaatc acgccaccag gaaagcagta
    22801 ggcttcaatc gtgttatttt ttaccagcgc ctgcattttg ggcgaccagg taaagtgccc
    22861 ggcaataatg cgtttgagca tctctgcgtg cgcaaaacga ttagtgcctt tgcagtcgcg
    22921 atcgccaata cccagcgagt ggattagcgt caggtcgcga gggtgtccgg tttgcaggaa
    22981 acgcgcttca atagcagcca gaatatggtc ggcttccacc ataccgccgc cgttaccgct
    23041 aatagcaata gtcgcaccgt cctgaatttg cgccgccagt gcctcggcgg ttgttatctt
    23101 acgcatacaa tttccttatt gattatggaa gctcacgcta cttttttcgg caaaggcgcg
    23161 cgtcgcctgc tggcaatcct gagtggcgga cattaatccg gccagtagct ggtgcgggta
    23221 atcggcatgt ttattttcca gtgccagaat cagttgctta atatgcgcca gtgccaccgg
    23281 accgcatttt tccagctgcg ctaactggtt caccatccag ctttccacct gttctttttc
    23341 taccacttca tcaattaaat tcgccgcctg agcttcctgc gccgtcaggc gtttacccag
    23401 catcagcatc tggcgaccga ccaccggacc aacctgatta agcacacgtt caatgcccat
    23461 ccagccaggc accatgccga gcatcacttc cgggttagag aatttcgcgc cggggcgggc
    23521 aatacgaata tcgcagcaca aggccagttc aataccaccg ccgatggtat gaccgttgag
    23581 attcgcaacg gttaattggg gtaagtttcg caggcgatta aaaacgtcat taccgcgttt
    23641 aatccatttt ctgcccatat ctaatggcga ataggctgac cacgatttaa tatcgccgcc
    23701 cgcacaaaag aatttatcgc cggttgccgt taattcgact aatcgtaatg tggtgtcgtt
    23761 ttcaatttca tttaaataat ggtcgagaga ttcgatcatt tcttcattaa tagcatgaca
    23821 tttatcttca cggttcaatg tcaggcggca ggaagctgcc gctctgctga acagaacggg
    23881 ttggtcactc atgatcattc cttaacaata atgcgcgatg cgatcgacgc taatatccag
    23941 cgtgctgtta atatctttct gccaccagta aggcgagaat atttccagtt ctatagcgcc
    24001 attaaatccg gcgttttcaa ccagccgacg aattgacgga atattgatga cgccatctcc
    24061 cggcattccc cggtcattga ccagatcggt ggtcggtact aaccagtcgg aaacatgaaa
    24121 cgcgaggatg cgttttccag cacgcaggat ctgactggcg agatccggat cccaccagac
    24181 gtgataaaca tccaccgcca cgccgaggcc aaactcacca tcgggatcga gttcatcaca
    24241 ccagtccagc gcctgacgta acgtgcacag gcaagagcga tcggcagctg tcatcgggtg
    24301 cagtggctcc agcgcaattg gcacgccgac atctttcgaa tgtggcagca actggcgaat
    24361 cccttgtttc acttgttctc gcgcttcata tagatcttta gtgcccgttg gcagaccgcc
    24421 aaccacctgc atataacagg cggcgttaag caccgctgcg tcgtccagcg cccggcggtt
    24481 atcatcaatc gccagtttgc gttccgccag tgtcggcgcg gtgtagtaag tgctacggca
    24541 caaaccggaa acgctcatgt tactggcggc aagctggcgg gtgatttgct ggagatcttc
    24601 accctgcaac tccctgcgcc agggggcaat tgcgccaata ccgcgtgcgg cgcaggcatc
    24661 aataatggcg ggtagcggcg ttttatagcc cagtgtcgcg gtattgatac tgaataaatc
    24721 cgggcgttgt aacagatctc tcatgattca gcctcagaag cgcggcatac ttaagccgcc
    24781 gtcgatggcg accgcttgac cacaggtgta aatcagttta ccttccgcca tcgcgcggac
    24841 ggtggaagca atatcagccg gataacccca acgtccccac ggcaccagac ctttggcaat
    24901 cagttcgtcg taataagccg ttgctggaat agtcatatcg gttttgatga gaccagggcg
    24961 cacttcataa acgccaattt gttcattaca aaggcgagca gcaaacagac gagcagcggc
    25021 agaaacggcg gttttggcaa tggtgtattc gccgcgattc atcgccagca tgatggcgtt
    25081 gatggaactg acgaaaataa tcgagcggtg cggcagctcc gcttccggtt tcggctgtgc
    25141 cagtaagcgc ttactgaatg cctgagcaag gaagaacggc gcgcgggtgt taatggcaat
    25201 gttttgatcg aagctgtctg gttcgaggtc gaggaggtca ccgcgttttt tcactgaaat
    25261 gcctgcgttg ttgagcaggc agtccagacg tccccacagg ttttgcgccg cgtcgagcat
    25321 ctcttcatgc aaggagagat cgccgacatc ggcagggaag caaatcactt ccacgccttc
    25381 agctatacat tcttgttgtg tgatatgcag tttttctacg ctgtcggcat cgggaagatc
    25441 gttaatcagc aggttaaatc cgccacgagc aagctccagc gcacagccct ttccaatacc
    25501 acgagcggct ccggtaatca cggcaacagg acgagtggat ttcatacaat agctctcttt
    25561 ttttgaatat tgttttctaa gtgacaatta gcttgcgacg tgccgcaaga aagatcaacc
    25621 atctggttgg ttgatatgcg atagtgatca caggacattt gataacttga tgaaagtaca
    25681 agtttttatc ctttttactg gagcttctaa ttaatcgggt taagatagtt atcttgaaga
    25741 ggtcgtgggg aagcgttatg tatcttgata ccagcaacgt acagagcctg aaggaaaagc
    25801 tgctgttatg cgcggttaat gaatttgccg aatatggtta tgaaggggcc agggtcgata
    25861 atattgttaa ggct
//
LOCUS       DAFNBJ010000064        25818 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000064 DAFNBJ010000000
VERSION     DAFNBJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.064"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..476
                     /locus_tag="L2X49_002443"
                     /pseudo
     CDS             1..476
                     /locus_tag="L2X49_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Accessory colonization factor AcfD"
     gene            476..679
                     /locus_tag="L2X49_002444"
                     /pseudo
     CDS             476..679
                     /locus_tag="L2X49_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
     gene            682..1659
                     /gene="gspK"
                     /locus_tag="L2X49_002445"
     CDS             682..1659
                     /gene="gspK"
                     /locus_tag="L2X49_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HBN4797520.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            1656..2834
                     /gene="gspL"
                     /locus_tag="L2X49_002446"
     CDS             1656..2834
                     /gene="gspL"
                     /locus_tag="L2X49_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HBN4797521.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPVVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADFVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            2836..3372
                     /gene="yghD"
                     /locus_tag="L2X49_002447"
     CDS             2836..3372
                     /gene="yghD"
                     /locus_tag="L2X49_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HBN4797522.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETILQQLVAMNTRLKSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIRRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(3521..3596)
                     /locus_tag="L2X49_002448"
     tRNA            complement(3521..3596)
                     /locus_tag="L2X49_002448"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3561..3563),aa:Phe,seq:gaa)
     gene            complement(3702..4409)
                     /locus_tag="L2X49_002449"
     CDS             complement(3702..4409)
                     /locus_tag="L2X49_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBN4797523.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            4807..6942
                     /gene="speC"
                     /locus_tag="L2X49_002450"
     CDS             4807..6942
                     /gene="speC"
                     /locus_tag="L2X49_002450"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBN4797524.1"
                     /translation="MKSMNIAASSDLVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTMRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(6992..8248)
                     /gene="nupG"
                     /locus_tag="L2X49_002451"
     CDS             complement(6992..8248)
                     /gene="nupG"
                     /locus_tag="L2X49_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBN4797525.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(8450..9529)
                     /gene="mltC"
                     /locus_tag="L2X49_002452"
     CDS             complement(8450..9529)
                     /gene="mltC"
                     /locus_tag="L2X49_002452"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBN4797526.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(9594..9869)
                     /locus_tag="L2X49_002453"
     CDS             complement(9594..9869)
                     /locus_tag="L2X49_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBN4797527.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(9897..10949)
                     /gene="mutY"
                     /locus_tag="L2X49_002454"
     CDS             complement(9897..10949)
                     /gene="mutY"
                     /locus_tag="L2X49_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBN4797528.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            11110..11829
                     /gene="trmB"
                     /locus_tag="L2X49_002455"
     CDS             11110..11829
                     /gene="trmB"
                     /locus_tag="L2X49_002455"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBN4797529.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            11829..12155
                     /locus_tag="L2X49_002456"
     CDS             11829..12155
                     /locus_tag="L2X49_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBN4797530.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            12339..13058
                     /locus_tag="L2X49_002457"
     CDS             12339..13058
                     /locus_tag="L2X49_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBN4797531.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            13234..14280
                     /gene="ansB"
                     /locus_tag="L2X49_002458"
     CDS             13234..14280
                     /gene="ansB"
                     /locus_tag="L2X49_002458"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBN4797532.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            14397..15404
                     /locus_tag="L2X49_002459"
     CDS             14397..15404
                     /locus_tag="L2X49_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBN4797533.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(15559..16695)
                     /gene="hemW"
                     /locus_tag="L2X49_002460"
     CDS             complement(15559..16695)
                     /gene="hemW"
                     /locus_tag="L2X49_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBN4797534.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(16688..17281)
                     /locus_tag="L2X49_002461"
     CDS             complement(16688..17281)
                     /locus_tag="L2X49_002461"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBN4797535.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(17289..17579)
                     /locus_tag="L2X49_002462"
     CDS             complement(17289..17579)
                     /locus_tag="L2X49_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBN4797536.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(17576..18142)
                     /gene="yggT"
                     /locus_tag="L2X49_002463"
     CDS             complement(17576..18142)
                     /gene="yggT"
                     /locus_tag="L2X49_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBN4797537.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(18160..18864)
                     /gene="yggS"
                     /locus_tag="L2X49_002464"
     CDS             complement(18160..18864)
                     /gene="yggS"
                     /locus_tag="L2X49_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBN4797538.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            18882..19862
                     /locus_tag="L2X49_002465"
     CDS             18882..19862
                     /locus_tag="L2X49_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBN4797539.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGCVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            complement(20025..20441)
                     /gene="ruvX"
                     /locus_tag="L2X49_002466"
     CDS             complement(20025..20441)
                     /gene="ruvX"
                     /locus_tag="L2X49_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBN4797540.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(20441..21004)
                     /locus_tag="L2X49_002467"
     CDS             complement(20441..21004)
                     /locus_tag="L2X49_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBN4797541.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(21113..22063)
                     /gene="gshB"
                     /locus_tag="L2X49_002468"
     CDS             complement(21113..22063)
                     /gene="gshB"
                     /locus_tag="L2X49_002468"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBN4797542.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(22076..22807)
                     /gene="rsmE"
                     /locus_tag="L2X49_002469"
     CDS             complement(22076..22807)
                     /gene="rsmE"
                     /locus_tag="L2X49_002469"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBN4797543.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(22887..23594)
                     /gene="endA"
                     /locus_tag="L2X49_002470"
     CDS             complement(22887..23594)
                     /gene="endA"
                     /locus_tag="L2X49_002470"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBN4797544.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(23689..24186)
                     /locus_tag="L2X49_002471"
     CDS             complement(23689..24186)
                     /locus_tag="L2X49_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBN4797545.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(24263..25657)
                     /gene="galP"
                     /locus_tag="L2X49_002472"
     CDS             complement(24263..25657)
                     /gene="galP"
                     /locus_tag="L2X49_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBN4797546.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         6311 a         6586 c         6880 g         6041 t
ORIGIN      
        1 gtgatggcgg tggctccgga tcttcctccg atacgccgcc tgtagattct ggaacagggt
       61 ctttgccgga agtgaaacct gatccaacac caaacccgga gccgacacct gagccaacgc
      121 cggacccaga acctacgccg gaaccgacac ctgatcctga gccaacacca gaaccggagc
      181 cagaacctgt tcctacgaaa acgggttatc tgactctggg cggaagcctg cgggtaactg
      241 gtgatatcac ctgtaatgat gaatccagcg atggctttac ctttacacca ggcgacaaag
      301 tcacctgtgt ggcagggaac aacacgacaa ttgctacctt cgacacccag tcagaagctg
      361 cgcgtagcct gcgtgcggtt gaaaaagtgt cgtttagtct tgaggacgcg caagaactgg
      421 cgggttccga caacaagaaa agcaatgcgc tctcgctggt cacctccatg aacagttgac
      481 cgatgccgca ggcagcgtga agccgacaat gcaaaagttg attccggccg attcgctccg
      541 tttgcagttc tacgacggca cgcgctggca ggagagctgg tcgtcagtac aggcgatccc
      601 tgtggcggta cgcatgaccc tgcattcgcc gcaatggggc gagattgaac gcatctggtt
      661 gttacgcggg ccgcaataat catgatcacc tcaccaccaa aacgcggaat ggcactggtt
      721 gtggtgctgg tattgctggc agttatgatg ctggtaacca tcacgctttc cgggcggatg
      781 cagcagcaac tgggaagaac gcgcagccag caggagtacc agcaggcgct gtggtacagc
      841 gccagtgcag aaagcctggc gttgagcgcg ctcagtctga gcctgaaaaa tgaaaagcgc
      901 gtgcatctgg cacaaccgtg ggcttccggc cctcgttttt tcccactgcc gcaggggcaa
      961 atcgccgtca ctctgcgtga cgcacaggcc tgctttaacc tgaatgccct cgctcagccc
     1021 acaacggcgt cgcgtccgct cgcggtacaa caactgattg ccctgatcac gcgcctggat
     1081 gtgcctgctt atcgggccga actgatagcc gaaagcctgt gggagtttat tgatgaagac
     1141 cgcagcgtgc agacgcgtct gggccgtgaa gacagcgaat atctcgcccg ctcggtgcct
     1201 ttctacgccg ccaatcaacc gctggccgat atcagcgaga tgcgcgtggt gcagggaatg
     1261 gacgccgggc tttatcaaaa actgaaaccg ctggtctgtg cgctgccgat gacccgccag
     1321 caaatcaaca tcaatacttt agacgtcacg caaagtgtga ttcttgaggc gctgtttgac
     1381 ccgtggttaa gccctgttca ggcgcgggcg ttattacaac aacgtccggc gaagggctgg
     1441 gaagatgtcg atcagtttct tgcacagccg ctacttgctg acgtcgatga gcgtactaaa
     1501 aaacagctaa aaaccgtcct gagcgtggac agcaattact tctggctgcg ttcagatatc
     1561 accgtgaatg agattgaact gacgatgaac tcgttaattg tccgcatggg cccacaacac
     1621 ttttcggttc tctggcatca gacaggagaa agtgagtgag ttccatcctt gagatttttt
     1681 tcccgctttg cgccgctgat cccatccatt ggcagcgccg tacacccgat gtggagcacg
     1741 gtatctggtc tgacgttgct aacgaacagc tccagcaatg gctgcaaact gatgcgattc
     1801 gactctacat tcccggcgaa tggatcagcg tctggcaggt tgaactgcct gttgtcgccc
     1861 gcaagcagat accgactatt ctgcccgcct tactggaaga agagctgaac caggatatcg
     1921 atgaactgca tttcgccccg ttgaaaatcg accagcaact ggcaaccgta gcagtgattc
     1981 accaacagca tatgcgcaac attgcgcagt ggttgcagga aaacggcatc acccgcgcta
     2041 ccgtcgcgcc ggactggatg tccattcctt gtgggtttat ggctggcgat gcgcaacggg
     2101 ttatctgccg catcgatgaa tgccggggat ggagcgccgg gctggcgctg gctccggtca
     2161 tgttccgcgc ccagctcaat gagcaggatt taccgctttc gctaaccgta gtcggcattg
     2221 caccggaaaa gctatcagca tgggctggcg cagacgctga acgcctgacc gttacagctc
     2281 tgcccgccgt taccacttat ggcgaaccgg aagggaacct gctgacaggg ccgtggcagc
     2341 ctcgcgtcag ctaccgaaaa cagtgggcgc gctggcgggt gatgattctg ccgatattgc
     2401 tgattctggt tgcgctggca gtggagcggg gcgtgacgtt atggagcgtc agcgaacagg
     2461 tggcgcaaag ccgcacccag gcggaggaac agttcttaac gttgttcccg gagcagaagc
     2521 ggattgtgaa tttacgctct caggtgacga tggcgctgaa aaaatatcgc ccacaggccg
     2581 acgatacccg gctgctcgcc gagttgtcag cgatagccag caccctgaaa tcagcgtcac
     2641 tttccgacat cgaaatgcgt ggtttcacct ttgatcaaaa acgccagacg cttcacctcc
     2701 agttgcgggc cgcgaacttt gccagcttcg acaaactgcg tagcgcactg gcggcagatt
     2761 ttgttgtgca gcaggacgcg ttacagaaag agggtgatgc ggtttccggc ggcgtaacgt
     2821 tgcggaggaa ataacatgtt acgcgataaa tttattcact attttcagca atggcgtgaa
     2881 cgccagttaa gccgtggcga acactggctg gcacaacacc tggcggggcg ttctccgcgt
     2941 gaaaagggca tgttactggc agcggtggtg ttcctgttta gcgtcggata ttacgtcctc
     3001 atctggcagc ctttgagcga acggattgag caacaggaga caatattgca gcagctggtg
     3061 gcgatgaaca cccgactgaa gagcgccgcg ccggatatta ttgcggcgcg aaaatccgcc
     3121 acaacaacgc ctgcgcaggt atcgcgggtt atcagtgaca gtgcttccgc gcactcggta
     3181 gtcatcaggc ggatagccga gcgtggggag aatattcagg tctggataga acctgtggtg
     3241 tttaatgacc tgctgaaatg gttaaacgca ctggatgaaa aatatgcgct gcgggtgaca
     3301 caaattgatg tcagtgctgc tgagaagcct gggatggtga atgtgcagcg gctggagttt
     3361 ggacgggggt aaagtatcag gagattaata tcaataaaat tactctcgtt aacgtggcga
     3421 gagtaattcc cccagacttt ataagctaat ttttttcgtg aaggttattg tcagatccaa
     3481 aagcaaaaac ccgccttgtg ggcgggttct taagaattag tggtgcccgg actcggaatc
     3541 gaaccaagga cacggggatt ttcaatcccc tgctctaccg actgagctat ccgggcaacg
     3601 gggcgcatta aacctgattc gcctcgtctc gtcaatcaaa tttcttcaat ttactgcaga
     3661 ctgcacaatc tatcatcact ttgccgctat tgcacgcatt gtcaggcaaa ccaggcggtc
     3721 caggcggcgg aaagcggcat tgccagaagg agtgctggaa gcatgttgac caccgggaac
     3781 attttaatgc cacagatgcg taatccggtt gccagcagca ataaaccgcc tacggcgctg
     3841 aagtctgcca tcatcgacgg tgtggtcagc ggtaatatca gcgcggcagc ccacgccagc
     3901 gttaactgga tgatcagtaa tgggatacta attaccgaca ccgcaatacc cagcgagcag
     3961 gcgaagatca tcgccgtaaa gaaatcaaga aatgacttgg cgattaaaat actcggatcg
     4021 ccggtcatcc cttcgttcat cgccccgaag atcccggtgc cgctggcgca aaacaggaca
     4081 ataatcgcga cataattctg aataaaagat tcatgcgctg gcttcttacg tgagtgacga
     4141 aacagatttt gtgctttggt gactgctgta ttgacacctt tctccagcaa acaaatttcg
     4201 ccaattagag cgccaagtag cgtagctaaa accatcgctg gaaggttggc acatttcacc
     4261 accagtaaaa taccaatccc cagcgatgcc agaccaaaaa tagatgtcat ggagacgcgg
     4321 atacgttccg gtaagcgttg gctgagcagt gcgccgagaa cgccacccag taatactgca
     4381 ctagcgttga taaaagggcc gatgaccaca agagttcctg ttaactgtat tgtttgattt
     4441 tcttattatg accgtttggt gccgtcgatg gcttgtttgt tcgcaaagtc ctggcttgca
     4501 cgctttagcg aaaggtgcca tgattgcgcg aattttctcc tctctgtacg gagtttgccc
     4561 gatgcacgcc acctccttac attctctcgc ttatcgccgt ttcgcgcgaa acgtttccct
     4621 ttctatgtta ctgctcatgc ggtgaagtta acgcacgctc actgcaggac aacagtaaaa
     4681 tcagagcgtt tctgctttta ctgatgtctg gcggtcggag ctggtgacca gtttgaccca
     4741 tatctcatgg ggcagggttt tccaccttgt ccggtattct tacttccccg aaacgggttt
     4801 gcgcttatga aatcaatgaa tattgccgcc agtagtgatc tggtatcccg actttcttct
     4861 catcgtcgcg tggtggcgtt gggagatact gattttacgg acgtcgcggc agtcgtcatt
     4921 accgctgcgg atagtcgcag tggcattctt gcgttgctta agcgcaccgg ttttcatcta
     4981 ccggtgtttt tgtattccga acatgctgtt gaattacctg cgggcgttac ggcggtaatc
     5041 aacggcaacg agcagcaatg gctggagctg gaatccgcag cctgtcagta tgaagagaat
     5101 ttgctgccac cgttttatga cacgctgacg cagtacgttg agatgggcaa cagcaccttt
     5161 gcttgccctg gacatcaaca tggtgcgttt tttaaaaagc atcctgccgg acgccatttt
     5221 tacgatttct ttggtgagaa cgtctttcgc gccgatatgt gtaacgctga cgtaaaattg
     5281 ggcgatctgc ttattcatga aggatcggcg aaagatgcgc agaaattcgc agccaaagtc
     5341 tttcatgccg ataaaaccta ttttgtgctg aacggcacat cggcagcgaa taaagtggtg
     5401 acgaatgcgc tgttaacgcg tggcgatctg gtgctcttcg accgtaacaa ccataagtcg
     5461 aatcatcacg gcgcgctgat tcaggcgggg gcgacgccgg tctatctgga agcttcacgc
     5521 aacccgtttg gtttcattgg cggtattgat gcgcactgtt ttaatgaaga gtatctgcgc
     5581 cagcaaattc gcgacgttgc gccagaaaaa gctgagttgc cacgcccgtt tcgcctggcg
     5641 attattcagc tgggaaccta tgacggcact gtctataacg cccgtcaggt gatcgatacc
     5701 gttgggcatc tgtgtgatta cattctgttt gattccgcgt gggtcggtta tgaacaattt
     5761 atcccgatga tggcggatag ctcgccgctg ctgttagaac ttaacgaaaa cgatccgggg
     5821 atctttgtga ctcagtcggt gcacaaacag caggcgggat tctcacagac gtcgcagatc
     5881 cataaaaaag ataaccatat ccgcggacag gcgcgttttt gcccgcataa gcggttgaat
     5941 aatgccttta tgctccatgc ttctaccagc ccgttctacc cgctgtttgc cgcgctggac
     6001 gttaacgcta aaattcatga aggagagagt gggcgtcggc tgtgggctga gtgcgttgag
     6061 ttgggtattg agtcgcgcaa ggctattctt gcgcgctgta agctgttccg cccgtttatc
     6121 ccgcccgttg ttgatggcaa attgtggcag gattatccga catctgtgtt agccagcgac
     6181 cgccgttttt tcagttttga gccgggggcg aagtggcacg gctttgaagg atatgccgcg
     6241 gatcagtatt ttgttgatcc gtgcaagctg ttactcacta caccaggtat caatgccgaa
     6301 actggcgaat atagcgactt tggcgttccg gcgacgattc tggcgcacta tctgcgtgag
     6361 aacggcattg tgccggagaa gtgcgatctc aactccattc tgttcttatt aactccggcg
     6421 gaaagccacg agaagctggc acaactggtg gcgatgctgg cgcaatttga acagcatatt
     6481 gaggatgact cgccgctggc tgaggtgttg ccgagcgttt ataacaaata tccggtgcgc
     6541 tatcgcgact acaccatgcg ccagttgtgt caggagatgc acgatctgta tgtcagtttc
     6601 gacgtcaaag acctacaaaa agcgatgttc cgccagcaga gtttcccgtc agtggtgatg
     6661 aacccccagg atgcgcatag cgcttatatt cgcggtgaag tggagttggt gcggattcgt
     6721 gatgccgaag ggcgaattgc ggcagaaggg gcgttgcctt atccacctgg cgtgctttgc
     6781 gtggtacccg gggaagtctg gggtggggcg gttcaacgtt atttccttgc actggaagaa
     6841 ggggtgaatt tgttgccggg attttcgccg gagctgcaag gtgtttatag cgaaaccgat
     6901 gcggatggca tgaaacggtt gtacggttat gtgttgaagt aagaataaaa aaaacgggtc
     6961 accttctggc gacccgtttt tctttgcgta attagtggct aaccgtctgt gtgcctgtcg
     7021 ggacacgaac gtgtttatat ttgaacatcg ccatgaacgc gaaggccaga accacggagt
     7081 aaccagcgaa aatcaaccat acggtctgcc agtcggtaat gccgttttgg gtgtacatct
     7141 caacaacttt accgctcacg atgccgccga ggatacagcc gaagccgtta gtcatcatca
     7201 ggaacatccc ttgtgcactg gcgcgaattg ccgggctaac ttctttttcg acaaacaccg
     7261 aaccagagat gttgaagaag tcgaatgcgc aaccgtaaac gatcatcgac agtaccagca
     7321 gtacagtacc gaacggagtc gggtcgccgt aagcaaacag cgcaaaacgc aggatccacg
     7381 ccacaatact gatcatcatt acgttcttaa taccgtagcg gcttaagaag aacgggatgg
     7441 tcagaatgaa cagggtttca gagatctgcg aaatcgacat gatgattgac gcatgctgca
     7501 caataaagct gctggcaaac atcggatctt tgtcgaagct gtgcaggaag gtattaccga
     7561 acatgttggt aatctgcagt tccgcgccca gcagcattga gaagataaag aagattgcca
     7621 tacgcttgtt tttaaacagc gcgaatgcat cgaggcccag cagggttgtc cagctctgat
     7681 tcgcttgctg tttagcaacc ggaatatgcg gcagagtcag ggtaaacaga accagaatgg
     7741 cggaaagtgc tgcgccaata tacagctgca tgtggcttaa ttcgaagcca gacaggctca
     7801 ccacccacat tgccatgata aagccgatgg tgccccagat acggattggc gggaagtcag
     7861 taacgatatc catcccggca ttttgcaggc gatagtaaga gatggtgttg attaacccaa
     7921 gcgttggcat ataagcaaac gagttaatca atatcacaag gaacatcgcc tccggtgtcg
     7981 tgacctgtgc cgccatgaac agcgtgatag cgccaatggt gtggcaaatg gcatataccc
     8041 atttcgcact taaccatttg tcggccacaa tccccagcag cgcaggcata aataccgcgg
     8101 cgatacccag tgagctataa actgcgccaa tagaagcacc gtcaaacttc agggtaacaa
     8161 acatatagga gccgagggtc gtcagccaac ttccccacag acagaactgc agaaaagaga
     8221 ggattttcag ctgcagctta agattcatgt taatttcctc acatcgtgat gcggatgagt
     8281 gtttgaaaaa gcacatttga ccgtgcgaga tgatgggatt ctatcaactt cgcggactgg
     8341 ttttttgtta cctgtggcaa ataattgcaa acattcccaa tttgcaaaac gaaattgtga
     8401 tgtggataac atttttgccc ctgagcatcg tcaggggcgg ttaatggaat tatcggcggc
     8461 ggtaggattt ttgcgcggta ttcactttat aaagataacg gcgagattcc gcagagggat
     8521 ggcgggtcgt cagcgtctga taaacatcgc ccggcgtcat ggtgttaata atattggcag
     8581 cctgaatctt atcattcgaa aagactcgca gcacgctgcc tgcgccgccg ttataggcgg
     8641 tgatgacggc ataacgccgc gatgttgggt tatcaattcc gccgagataa acattgttca
     8701 gcatcgccag atacgcggtg ccggtatcaa tattgctggc aggatcaaac aagaaactgc
     8761 ggctcggcgt gccggatttc ccctgcgagc ggaacacatc tttcccggca gtatgttgta
     8821 ccacctgcat taatcccagc gcatcggaac ggctgaccgc atatgggtta aaggaagatt
     8881 cggtctgcat aatcgccaga atcagcgact catcaacgcc atatttccgt gacgcctggc
     8941 ggaccatgcc gagatatttg tgcgcacgtt tatcaaggtg gttcggcacc atgttaatgg
     9001 taacgctgta gatgatacgc agcccgttgc tgcggctctt cagacggttt ttcagcagat
     9061 aatccgcgaa gttgctcgcg cgaccttccc agcgaatcgg ctgcccggtg ttgtccacca
     9121 cctgaccgta aaggaaaggt tctttcgaaa tcgtaatatc atcaacgtcg gaatagagat
     9181 cgaccgaact cggatcgtca cccatcagca acgttttgat aattgcccgg cgcaaatgcg
     9241 cggcaggttc tgtcccggcg atagtttcga tagtaatcgt accgtcatcg aagttgatgt
     9301 ggctgcgggt ctgatattga tcggtgtatt tcacgtagtc cttaggaccg gcgatcacca
     9361 cctctttgaa tccccagatg ttctcaatat tgtgggcaaa ttgccccatc agaatatcaa
     9421 aaccgttggt atctttgacc caggcttcgt tataggtatc gccttttttg gtcgtcgaac
     9481 aggagatgag caacggcgca atcaaagcca gcgcgagata ttttttcatc attccgggtg
     9541 cgtgttgtgt ttgtttttat gcaagttgcc ggaggcgtgc tccggcactg tttttatttt
     9601 ttatcttccg gcgtatagcc ctcgatatgc acctctttac cctcgaacag gaagttgacc
     9661 atctcctgct ccagcagctt gcggtgctcg gcattcatca tgttgagttt cttttcatta
     9721 atcagcatgg tttgtttgtg ctgccactgc gcccaggctt ctttggagat ctcgttatag
     9781 atgcgttttc ccagctcgcc ggggtacagc tgaaaatcct gaccttctgc ttcacgttgc
     9841 aggaaagtac aaaaaatcgt tctgctcata aatcatcctc tttatcgact cacgcgctaa
     9901 accggcgcgc cagtgcgtaa ctgctgtaac aaacgctcca cgggagccgc caggccaact
     9961 gacggcggtt gcgctaagtt ataccagagc gcattgcctt catccatgca gccggtgaat
    10021 gacgacacgg gaagccacat aggcacaata tctaagtgga aatggctgaa ggtatgccga
    10081 aacgcggtca gctgcgtcag gttatcggca gcaatctgcc gttgcgccag ccactgccgc
    10141 aaactttctt cgtcggcaaa ctgcgggaaa cagtataaac cgccccacaa tccgctcggc
    10201 ggacgctgcg ccagcaatac ttcatcttcg tgctgtaaca ggagaaagta gccggtgcgc
    10261 tccggcagcg tctgtttcgg ttttttgccc ggataaagcg accagctatt gttggcggcg
    10321 gcaatacatc cgttttgtag cggacagagc gaacatttcg gtttcgagcg cgtacaaatc
    10381 atcgcaccca aatccatcat cgcctgatta aaccgttcca cgccaaccgc gggcgtcacc
    10441 tgctcgctca aactccataa tttattctcg acctcttttt tccctggcca gccgcttaca
    10501 gcatagcagc gcgccagcac ccgtttgacg ttaccgtcga gaatcggaaa gtgcttaccc
    10561 agagaaagcg agagaatcgc acctgcggtg gaacgcccga cgcccggtaa cgccgcgact
    10621 tcttcaaagg tttccgggaa tttaccgccg tgtaaggtcg ccacttgttg tgccgcttta
    10681 tgcaggttgc gcgcgcgggc gtaatagcca agcccggtcc acaagtggag aacttcgtcg
    10741 agcggcgcat tggcgagatc ggtcaccgtc gggaagcgcg ccataaagcg ttcaaaatag
    10801 gggataacgg tcgcaacctg agtttgttgc aacatcactt ctgagagcca tactttgtag
    10861 ggcgtcttgt caatttgcca gggcagcgtt ttccgcccgt atttatcgta ccagtccaga
    10921 acctgggctg aaaattgcga cgcttgcatg gtcaccgaat tcactgttgt tgggggcaag
    10981 attgcagcac agcgacggca gggtgtaaac cggaactttc cgcagccacg gccttatcat
    11041 gcttgcatcc ggcaactaac tttggataat gcccgttttc agaacacttt cacaagcgac
    11101 taaatcttta tgaaaaacga cgtcatttca ccggaatttg atgaaaacgg ccgcccactg
    11161 cgccgtatcc gtagttttgt gcgccgccag gggcgactga ccaaaggcca ggaacatgcg
    11221 ctggaaaact actggccggt gatgggcgtt gagttcagcg aagatatgct ggatttcccc
    11281 gcgctttttg gccgtgaagc gccggtgacg cttgagattg gttttggcat gggggcatcg
    11341 ctggtggcaa tggctaaaga tcgccccgag caggacttcc tcggcattga agtgcattca
    11401 ccgggcgttg gtgcgtgcct ggcttctgcg catgaagaag gtttaagcaa cctgcgagtg
    11461 atgtgtcacg atgcggttga agtgctgcat aaaatgattc ctgacaattc attgcgcatg
    11521 gtgcagctct ttttccctga cccgtggcac aaagcgcgcc ataataaacg ccgtatcgta
    11581 caggtgccgt ttgccgaact ggtaaaaagc aaactgcagc tggggggggt attccatatg
    11641 gcgaccgact gggaacctta tgcggaacat atgcttgaag tgatgtcttc tattgacggt
    11701 tataaaaacc tgtcagagag caatgattac gtaccgcgtc cggcatcacg tccggtgacg
    11761 aaatttgaac aacgtggtca tcgtcttggt cacggagtat gggacttaat gttcgagagg
    11821 gtgaaataat ggcaaagaac cgtagccgtc gtctgcgtaa aaaaatgcac atcgacgaat
    11881 tccaggaatt aggattttcg gtggcatggc gattcccgga aggtacatcg gaagaacaga
    11941 ttgataaaac cgttgatgat tttattaacg aggttatcga accgaacaaa ctggcctttg
    12001 acggcagcgg ttatctggcc tgggaaggtc tgatctgcat gcaggaaatc ggcaaatgca
    12061 ccgaagaaca tcaggcgatt gtgcgtaagt ggctggaaga gcgcaaactg gatgaggtac
    12121 gcaccagcga acttttcgac gtttggtggg actaagaaag catacgggcg atgacaaatg
    12181 caaaactgcc tgatgcgcta cgcttatcag gcctggaaag atgcacgatc gagtaggcgg
    12241 gataaggtgt ttacgccgca tccggcatgg aaaacgcgta ctttgttatc aatctggggc
    12301 cagcaaatgc tggcctgatt ttttcttgag ggaagactat gatgcgcaaa atgctgctgg
    12361 cggcagcact ttcagtgacg gcaatgaccg ctcacgccga ctaccagtgc agcgtcacgc
    12421 cgcgtgacga tgtgattgtc agcccgcaaa ccgtgcaggt gaagggcgaa aacggcaatc
    12481 tggtgatcac gccagacggc aacgtgatgt ataacggtaa gcaatattcc ctgaatgccg
    12541 cccagcgcga gcaggcgaag gattatcagg ctgaactacg cagcacgctg ccgtggattg
    12601 atgaaggcgc gaaaagccgc gtcgaaaaag cccgtattgc gctggataaa attatcgttc
    12661 aggagatggg cgaaagcagc aaaatgcgca gccgtctgac caaacttgat gcgcagctga
    12721 aagagcagat gaaccgcatt atcgaaacgc gcagcgatgg cctgacgttt cactataaag
    12781 ccattgatca ggttcgcgcc gaaggccagc aattagtgaa tcaggcaatg ggtggaattt
    12841 tacaggacag cattaatgaa atgggcgcga aagcggtgct gaaaagcggc ggtaacccat
    12901 tacagaatgt gctgggaagc ctgggggggc tgcaatcctc aatccaaacc gagtggaaaa
    12961 agcaggaaaa agatttccag cagtttggca aagatgtttg tagccgcgtt gtgactctgg
    13021 aagatagccg caaagccctg gtcgggaatt taaaataatc ctctatttta agacggcata
    13081 atactttttt atgccgttta attcttcgtt ttgttacctg cctctaactt tgtagatctc
    13141 caaaatatat tcacgttgta aattgtttaa cgtcaaattt cccatacaga gctaagggat
    13201 aatgcgtagc gttcacgtaa ctggaggaat gaaatggagt ttttcaaaaa gacggcactt
    13261 gccgcactgg ttatgggttt tagtggtgca gcattggcat tacccaatat caccatttta
    13321 gcaaccggcg ggaccattgc cggtggtggt gactccgcaa ccaaatctaa ctacacagcg
    13381 ggtaaagttg gcgtagaaaa tctggttaat gcggtgccgc aactgaagga cattgcgaac
    13441 gttaaaggcg agcaggtagt gaatatcggc tcccaggaca tgaacgataa tgtctggctg
    13501 acactggcga aaaaaattaa caccgactgc gataagaccg acggcttcgt cattacccac
    13561 ggtaccgaca cgatggaaga aactgcttac ttcctcgacc tgacggtgaa atgcgacaaa
    13621 ccggtggtga tggtcggcgc aatgcgtccg tccacgtcta tgagcgcaga cggtccattc
    13681 aacctgtata acgcggtagt gaccgcagct gataaagcct ccgccaaccg tggcgtgctg
    13741 gtagtgatga atgacaccgt gcttgatggc cgtgacgtca ccaaaaccaa caccaccgac
    13801 gtagcgacct tcaagtctgt taactacggt cctctgggtt acattcacaa cggtaagatt
    13861 gactaccagc gtaccccggc acgtaagcat accagcgaca cgccattcga tgtctctaag
    13921 ctgaatgaac tgccgaaagt cggcattgtt tataactacg ctaacgcatc cgatcttccg
    13981 gctaaagcac tggtagatgc gggctatgat ggcatcgtta gcgctggtgt gggtaacggc
    14041 aacctgtata aatctgtgtt cgacacgctg gcgaccgccg cgaaaaacgg tactgcagtc
    14101 gtgcgttctt cccgcgtacc gacgggcgct accactcagg atgccgaagt ggatgatgcg
    14161 aaatacggct tcgtcgcctc tggcacgctg aacccgcaaa aagcgcgcgt tctgctgcaa
    14221 ctggctctga cgcaaaccaa agatccgcag cagatccagc agatcttcaa tcagtactaa
    14281 tcacctcgcc ccggtatcgt gccggggctt tttcacttca gacttacgtc cattgccaat
    14341 tttcattacc ctaatgataa tcaccggaat aaattattcc gcgtgaggtt tttcgggtga
    14401 aaaagcaatg gattgttggc acggcactgc ttatgttgat gactggtaat gcctgggcag
    14461 atggcgaacc gccaactgaa aatatcttaa aagatcaatt caaaaagcag tatcacggca
    14521 ttctcaagct tgatgctatc accttaaaaa atcttgatgc taagggtaat caggccacct
    14581 ggtcagcgga aggcgatgtc tcttccagtg acgatctcta tacctgggtc ggtcagttgg
    14641 cagattacga actgctcgaa cagacctgga cgaaagataa accggtaaaa ttctcggcga
    14701 tgttaaccag taaaggaacg cccgcgtctg gctggtcggt gaacttttac tcttttcagg
    14761 cggcagccag cgatcgtggg cgggtggtcg acgatatcaa aacgaataat aaatatctga
    14821 tcgtgaatag cgaagatttc aattatcgct ttagtcagct tgaatctgcg ttgaataccc
    14881 agaagaactc gattcctgcg ctggaaaagg aagtgaaagc gcttgataag caaatggtgg
    14941 cggcgcagaa agcagcggat gcatattggg gaaaagatgc aaacggtaag caaatgaccc
    15001 gcgaagaagc ctttaaaaaa attcaccaac agcgtgatga gtttaataaa cagaacgata
    15061 gcgaggcgtt cgcggttaaa tatgacaaag aggtttatca accggcgatt gcggcatgtc
    15121 ataaacagag tgaagagtgt tatgaagtgc cgattcagca gaaacgagat ttcgatatca
    15181 acgaacaacg gcgacagact tttctgcaat cacaaaaact cagccgtaaa ttgcaggatg
    15241 actgggtaac gctcgaaaaa gggcaatatc cgttaacgat gaaagtttca gaaataaaca
    15301 gtaaaaaagt cgcgattttg atgaaaattg acgatatcaa ccaagctaat gaacgctgga
    15361 aaaaagatac cgaacaactg cggcgcaatg gggtgattaa gtaagtttga tatagtgcac
    15421 gatttgtagc aggatgcggc gttgaccccg catccggcaa tcaacgtctg atgcgacgct
    15481 tgcgcgtctt atcagaccta caaccgctgc cacatgtcgg ctggataagg cgttcacgcc
    15541 gcatccggca atacaagttt actcagccag aaaaagctcc agcagcgaat ttaaaaacag
    15601 cttcccatgt tccgttatct gccagtaatc cgcacattcg gtgagataac cctgggcaat
    15661 cgcctcgtct aactgtgggc gaatcacatc ttcgcaaagc ccggtatacg cactaaactc
    15721 tgcgcgtggc gcggcctcca gcaagcgaaa gcggttcata aagaactcaa acggcttatc
    15781 tgcggcttcg acatcacgct ggctttccag ataccttcct tgcataaaac cacgcggatg
    15841 acgcgtttta gtggtacgca gaatgcgccc atccgggaag gtcactttgc cgtgcgcgcc
    15901 gcagccaata ccgatgtagt cgccaaagcg ccagtagttg agattgtgct ggcactgata
    15961 actgggtttg gcgtaagcgg aagtttcata ttgctgataa cccgctgcgg ttaataactg
    16021 atgcccctgt tcgaatatat cccacagcgc gtcgtcgtcc ggcagtaccg gcgggcgtga
    16081 accaaacagc gtattaggtt cgatggtcag ttgataccag gaaagatgcg gcggattcag
    16141 ttcaatggcc tggcgtagat cgccaagcgc ctcttccagt gattgatccg gtagtccatg
    16201 catcaaatcg aggttaaagc tacgcagccc cagcccgctc gccagctttg ccgcgcgttt
    16261 cgcttcttgc gggccatgaa tacgcccaag tcgtttcagc ttttcttcgc taaaactctg
    16321 cacaccaata gagatgcggt tcacaccagc acgctgataa tcgacaaagc gatcggcttc
    16381 taccgtacca gggttcgctt ccatagtaat ttctgcatcc gctgccagcg gcaaacgcgc
    16441 acgcacgccg tccagcagcg tttgcatcgc cgggccggaa agcaggctcg gcgtaccacc
    16501 gccaataaag attgtcttta cttcacggcc ctgagcgtaa gccacatcat tgtccagatc
    16561 gcaaagcaga tgctgaacgt aatcgtcgtg cggcacttct cctttcaacg cgtgagagtt
    16621 gaaatcgcag tacgggcatt tctgcacgca ccacgggatg tgaatgtaga gactcagcgg
    16681 cggtaattta accattacgt aaagcgtcca acagcagttt caatgcctga ccacggtggg
    16741 aaatggcgct cttttcttcg cgggtcagtt cggcagcggt tttcccttcg gaaggtacga
    16801 agaagattgg atcataacca aagccaccag tgcccgctgg ttcacgagta atcacgcccg
    16861 gccagctacc gtggcacacc agcggagtcg gatcttccgc gtgacgcaga tataccagca
    16921 cgcagtggaa acgcgcctga cgttggtcgt ccggtacgtc tttcagtgtt tccagtagtt
    16981 tttgcagatt ctttagatcg gtcgcgtctt caccggaata acgcgcggag taaatgcccg
    17041 gcgcgccgcc aagcacatct accgccagac cagagtcgtc ggcaattgcc ggtaaaccgg
    17101 tcacttttgc cgcatggcgc gctttcagaa tcgcgttttc gataaaggtc aggccggttt
    17161 cttcagcgga atctacgccg aggtctgttt gggccacgat atcaagaccg aagtcgctaa
    17221 gcagcgacgc caactcacgc actttaccgg cattgccggt tgcgaggaca actttttgca
    17281 taggatacct aattaattaa cgccgcgatt tctggcggga tttgttgcgg attaatgatt
    17341 ttaatttgtt tgtggcggcc aagttcgcct ttttcaatca ccacctggct tttggcaacc
    17401 cggaattgct taccgagaaa cttcaccaga tgactgttgg cctggccgtc aaccggcggc
    17461 gcggtaatgg cgactttaac ttcgtcgcca tgtaaaccga caatagaatc acggctggct
    17521 ttcggctgaa tatagagccg taaaaccaga ccgtcatcat taactgttac ggcactcata
    17581 acgccatcca cagccccggc agcagcatgt ttccggttgc ctgtaatact tctgcgacac
    17641 ccatattgat gacatacagc agcagaacga ggatcatcgg cgagaaatca atcccaccca
    17701 ttgccggtag caggcggcga atcgggcgca gcagcggatc ggccagctga atcagcacgt
    17761 attcaatcgg actacgaccc tggcttaccc agctcataat cgccatcacc agcaggaccc
    17821 agaaaatcag caagccgatg gttttcagca gaatcagtaa accggcaatc cagatgattg
    17881 gcaggaaggt caccacttta aacagcacaa tggctttgat aaaactgaga atataggcaa
    17941 ccagcagcga ggcgctgtca atgggaccca ttgccggaat aacgcggcgc agtggcccga
    18001 taattggctg cgttaccttc actacaaact gtgagaaggg gttgtaaaaa tcacaatgag
    18061 cccactgcat ccagatgcgt aataacagca ccatggtata cagctcaatg accgttgaaa
    18121 gcaggaaagt caacgtattc atggcgttcc tttaattcct tattttttag agtaatcacg
    18181 cgcaccaaaa attgcagtgc cgatacgaac catcgtgcta cctgccgcaa tggcggcttc
    18241 catatcgtcc gacattccca gagagagcgt gtcgatatgc gggtagcgcg ttttcagtcc
    18301 ggcaaatgct acagccattt ggcgtgcaac ttcaaactgc cttacatatt ctgactcagg
    18361 cgcagggatt gccatcagcc cgcgcagacg taaacgcggt agttcagcga ccgccgctgc
    18421 cagctcgtcc agttctgcca gttgaatccc ggacttactg ttttcatcac taatattaat
    18481 ttgaatcagt acgttaagag ggggaagttc tgccgggcgc tgatcgttga ggcgggtagc
    18541 aatgcgcaaa cggtcgatgg tatgacacca gtcgaaatgc tctgccacca ggcggctttt
    18601 attagactgc aacgggccaa taaaatgcca ttctaatcct gttacgccca gttcctgaaa
    18661 gtggcgaatt ttatctaccc cttcctgaac gtagttttca ccaaattgac gctgcccggc
    18721 atcaatggct tctgcgatgg cgctcgcagg ttttgtttta ctgactgcaa gcagcgtaat
    18781 ttcttctgga gaacggccgc aacgcgttgc agccgctgag attttgtccc ggacctgtgc
    18841 caggttatgc gcaatatcgt tcattttccg aggatcttag tatgaatatg gaagaaattg
    18901 tggcccttag tgtaaagcat aacgtctcgg atctacacct gtgcagcgcc tggcccgcac
    18961 gatggcgcat tcgtggcaga atggaagctg cgccgtttga tgcgccggac gtcgaagagc
    19021 tactgcggga gtggctggat gacgatcagc gggcaatatt gctggaaaat ggccagctgg
    19081 attttgccgt gtcgctggcg gaaaaccagc ggttgcgtgg cagtgcgttc gcgcaacggc
    19141 aaggtatttc tctggcatta cggttgttac cttcgcactg tccacagctc gaacagcttg
    19201 gtgcgccacc ggtattgccg gaattactca agagcgagaa tggcctgatt ctggtgacgg
    19261 gggcgacggg gagcggcaaa tctaccacgc tggcggcgat ggttggctat cttaatcaac
    19321 atgccgatgc gcatattctg acgctggaag atcctgtgga atatctctat gccagccagc
    19381 gatgtttgat ccagcagcgg gaaattggtt tgcactgtat gactttcgca tcggggttgc
    19441 gggctgcatt gcgggaagat cctgatgtga ttttgctcgg agagctacgt gacagtgaga
    19501 caatccgtct ggcgctaacg gcggcagaaa ccgggcatct ggtgctggca accttacata
    19561 cgcgtggtgc cgcgcaggca gttgagcgac tggtggattc atttccggcg caggaaaaag
    19621 atcccgtacg taatcaactg gcaggtagtt tacgggccgt gttgtcacaa aagctggaag
    19681 tggataaaca ggaaggatgc gtggcgctgt ttgaattatt gattaacact cccgcggtgg
    19741 ggaatttgat tcgcgaaggg aaaacccacc agttaccgca tgttattcaa accgggcagc
    19801 aggtggggat gataacgttt cagcagagtt ttcagcagcg agtgggggaa gggcgtttgt
    19861 gacgttgcca tgcccgatgt gcaagcttat cgggcctaca aaggcaataa acgtctgatg
    19921 cgacgcttgc gcgtcttatc agacctacaa ccgctgccac atgtaggccg gataaggcgt
    19981 aaggcgcgat gatattatac actccagcag gcgtttaaat cgctttaata tccctgctcg
    20041 aaatagcttt cgagaataat aaccgcagag gcagagtcga ctttgccttt atttagcgcc
    20101 cgatagccgc cctgttcaaa cagaccggaa cgggcttcca cggtgctaag acgttcgtca
    20161 tggagcttta cttcaacacc gaaacgacca tgaatacggt tggcaaattt acgcgctctg
    20221 gcagtcaatg gttgctcggt gccgtccata ttcagcggca aaccgacgat gatttcgtcc
    20281 ggctgccact ctttcagtaa acgctcgata atgttccagt ccggcgtacc gtcctgtgct
    20341 ttaattgcag gcaaagggcg agcggtgccg gtaatgcgtt ggccgaccgc tacaccaatg
    20401 cttttggtac cgaagtcgaa ggcgagtaag gttccactca tcaggcgtgc cctgccacac
    20461 caggcatggt gagaatatcc acaccaatca gttttgccgc ctcgcgccag cggtcggcaa
    20521 tcggtgtttt gaacagaata ttcagatccg ccggggccgt tagccacgcg ttatcgagaa
    20581 tttcttgttc cagttgacct ttttcccagg aggcataacc cagagccacc aatacgtcag
    20641 acggttgttt atcggtgccg agcgtttcca gcacatcgcg ggaagtggtc atcactgtgt
    20701 tgtctgaaat gcgaatgctg gaagcaaaat tggagggcgg agtatgcaaa ataaacccgc
    20761 gatcttcagc cagcggaccg ccgagcataa ccggtttatc cagacggatt gattcatcac
    20821 gcggctccgg cgtgatcttc agcttttcca gaatcccttc aattttgaga ttttccagcg
    20881 gcttgttgac gatgatcccc attgcaccat tggtattatg ttcgcaaatg tagaccacgg
    20941 aacgacggaa aatcggatcc tggagagcag gcatggcaat aagaaagtga tgctgtaaat
    21001 tcattgtcag aggttctgtt tcctggttca aaaaagcaac agcgcccagt atgcgtggaa
    21061 agaagggcgc tgtcactcag agtctcaacg agatccttct cgctaaggtg ggttactgct
    21121 gctgtaaacg tgcttcgatg gcatccatta acattccggt gatcgacacc ggaaactctg
    21181 cttcaatctc acgaatacag gttgggctgg tgacgttaat ttcagtcaga cggtcgccga
    21241 tgatatccag accaacaaaa atcagccctt tttctttcag cgtcggcccg atctgacggg
    21301 cgattttcca gtcactttcc gtcagcggac gaggttcacc gcgtccaccg gcagccagat
    21361 tgccacgggt ttcgcccccc tgcggaatac gcgccaggca gtacggtacc ggctcgccat
    21421 ccaccaccag cacgcgtttg tcgccatctt taatggctgg caggtaattt tgcgccatgc
    21481 agtagcgagt gccatgctca gtcagggttt cggcaatcac gccgaggttt ggatcgcctt
    21541 ctttcacgcg gaaaatcgac gcgccgccca taccgtccag cggcttaaga atgatgtcgc
    21601 tgtgtttctc ccagaacgct tttagctgcg ctttattgcg cgtaaccagc gtttctggcg
    21661 ttaagtcaga gaaccaggcg gtaaacagtt tctcgttaca gtcgcgcagg ctctgcggct
    21721 tgttaacgat cagcgtccct ttctcttcgg cacgttccag aatataggtc gcgtagataa
    21781 actcggtatc aaacggcggg tctttacgca tcaggatcac atcgagatcg gccagcggca
    21841 gatcctgttc accgacgaat gaaaaccact cttcgtagtt ctgcttcacg ttcagcgtgc
    21901 gggtatgggc gcgggcttcg ccattgatca gatacagatc gcccatctcc atatagtgaa
    21961 gttcgtaacc acgacgctgt gcttccagca acatagcgaa actggaatct ttcttgatgt
    22021 tgatgtttgc gatggggtcc atcacgatgc cgagcttgat cattattctt ctccgttagc
    22081 ccaaatcgcc aaatcgtact tgtagcgcgg taatggcggt gagcgcagtt gtctctgtac
    22141 gcaaaacgcg aggtcccaac aggatatcag taaattgata gcgggcagtc atggcaattt
    22201 catctgccga taaaccgcct tccgggccaa tcagcaggcg gacgcgttca accggtaacg
    22261 gcaacgtatt gatgctgtta ctggcgcgcg ggtgaagatt cagtttcagt ccttcatcct
    22321 gctctgcaca ccaggcttcc agatccatcg ctgggcggat ttccggcacc cggttacgac
    22381 cacactgctc acaggcagca attgcaatct tctgccactg ctggagcttc ttgttcagac
    22441 gttcactatc cagtttaacg ccgcagcgct cagaaaaaag tggcgtaatg aggcttacac
    22501 cgagttcgat cgatttctgg atcgtaaatt ccattttttc accacgcgac atcacctgac
    22561 cgaggtgaat atgcagcgga gattcgcgat cgtcgatctt gccttccagc accttcactt
    22621 ccacgctttt tttgctggcg ctggtaattt cggcgtcaaa gacctggttg ctaccgtcaa
    22681 acaattgcaa cgcctgcccc ggccccatgc gcagtacgcg cccgatatgg ttggcggcat
    22741 cttcgcaaag cgcgatgtga gaatggctgg tcagtggttc aggatgataa atgcggggga
    22801 tacgcatagt taaaaatccg cgtcgtctcc ccacgcggtt gtacgcgtgg ggtaggggtt
    22861 aacaaaaaga gtcccgctag tgtaggttag ctctttcgcg cctggcaagc gcgttgcaca
    22921 tacgggttat gattgccttg caccttcgcg atgcgttcat cgcgctcgca ctcccagtcg
    22981 gtaaccggat acatcttgtt ccatgcgttg aacagctgcg tttgctggcg agagagtgtc
    23041 aggttgtatt ggtcgcgcat atagaagtag gtgcgcgcaa tggcaccgcg tgcacgcgct
    23101 ggtggttcgg cagctttttc tttgaaatcg accttcatgg cgcattgacc gtactggcct
    23161 tcgccgccat tccactggct gtacataaag ttgccgcgat cgccattcac ctcaccgact
    23221 gacggctgca ggttatgcat atcgctttcc atcttgcgat agaccggatc tttagcgcag
    23281 tttttacgtc caccgtcctg ccagcactgg cgctggtgac cgaactgcca ggcgggaacg
    23341 acgtgttccc actctacgcg gctggctcgg ttttcatttt tgcgcacctg atagccgcac
    23401 gattgcagat caacaacgcc ttttttgccc tgccagttaa ttttacatcc gcaataaaac
    23461 gtaccgggcg cgtcagcgtg gacttttacc gccgcggctt tcgcctgaga aaaactattg
    23521 ataccttcgg ccaacgccgg gccggaaaat gctgcgctca gtaccaccgc agcaatagac
    23581 aaataacggt acatcactta ctccgtgtta aaacgagcca gcaacgtagc gaaagttgtt
    23641 ttggtatgca atcagccaga tcaggaaagt tcctgatagt tcagatggtt atttcgcaac
    23701 cagctgttca ccgcagtgaa cacagcgata gacggcctcg ccacgaacta cgcgattatg
    23761 gcggcgtacg gtaagctgat gctcctggca cttgcagcgg taggggaagg tgttgcgacg
    23821 cacggattgc agttcgaact gatgcgtacg acgggcggga acgcccagca cgctttccat
    23881 catccacttc cactctttgc catgtggcgc tacgcggccg aaatgtttcc ataccagcaa
    23941 atgtgccagt tcgtgcggta ccacttcttc aataaaagct tcactgtttt ccaacagcaa
    24001 aacgggattg aggcggattt catagctttc cagccaggct gttccggcgg aggttccgcg
    24061 ctgggtgtaa gagagttttg gctccgggta gttacgccct agcttcaggt tggcctgggc
    24121 gagtttttcc cgcaggcggc gcataacggc ctgctggatg gcgataggga gacgggatgt
    24181 tttcatagcg gcagaggata gagcgaagaa aagatgactg caagaggtgg cttcctccgc
    24241 gatgggagga agcctgagga gattaatcgt gagcgccgat ttcgcgcagt ttacgacctt
    24301 tcatcagatt acgttcaata tgttccagcg aaacgtgttt ggtttccggt accagccaca
    24361 atgtcagcag gataaacagt acgttcagag ccgcatacac ccagaaggtg ttggcgttac
    24421 ccagcgtgtt gagcatggtc aggaacgttg cgccaacgat catgttggca atccagttgg
    24481 tggcggtgga gcaggtgatg ccaaaatcgc ggcctttcag cggctgaatt tcggagcaca
    24541 gtacccaaat cagcggaccg gcactcatgg caaaaccgac aataaacatc agcagcatgg
    24601 cgatggcgaa atactgcgct gacggagagt gaataccgat atgcatcatt gtaccgagta
    24661 cgcccatgcc agcagccatc accaggaagc ccagcgttag cgttggttta cgtccccagc
    24721 ggtcaacaag gccgattgcg ataaaggtgg caagtacgtt ggtcaggccg acaatcacgg
    24781 tcccccacat ttgctcggta gtgttggtat aacccgccag ttcgaagatt ttcggcgcgt
    24841 aatacatgat gacgttcatc ccggtgaatt gctgcattac ctgcaacagt acgccaagga
    24901 acaccgcgcg gcggaagttg ctgttctctt taaacagcgc ccagccactc tgtttaacct
    24961 gcaaactttc acggatttca tccagttcgc gtttcgcttc cgcgctggtg tcacgcaggc
    25021 gtagcagcac gcgttcggca tcaacaaaac ggcgcttggc ggcaaaccaa cgtgggctgt
    25081 ctggcaggaa gaagacacca atcagcagca aaattgccgg gatgataatc acacccagca
    25141 tccagcgcca tgcaccggtg tagctgaagg cggtatcaga aagataagca ccgaggatcc
    25201 cgatagtgat catcaactga tacatcgaga tcatactgcc gcgaattttt tccggcgcaa
    25261 tttctgacag gtacagtggt gcggtataag aggccacacc caccgccagc cccagcagaa
    25321 cgcgggaaag aatcagtact tcaacgtttg gcgcagccgc agagaacagc gaaccggcaa
    25381 caaacaaaat tgcgccgatc atcaggctct ttttgcgccc gagtttaaag gagagccagc
    25441 cgctgcccac cgcaccgact gccgcaccga acatcatgga gcttacgacc cattcttgcg
    25501 tgtgcgaagt aatctggaat tcatctgcaa taaacggcag tgcgccagca attacaccga
    25561 tatccaggcc aaagagtaat cccgccagag cggcaaggaa gcagacgaaa aaagtcattg
    25621 ccttgtttga ccgcccctgt tttttagcgt caggcatgat gccctccaat atggttattt
    25681 tttattgtga attaagatag gtgagtacga cgtaaaaaga tgtgaagcaa atcacatcag
    25741 tgtaatcgct tacactaaca tttatcttag ttatggttta agtgcatgaa aataattgag
    25801 ttatatcatc atagatta
//
LOCUS       DAFNBJ010000065        24460 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000065 DAFNBJ010000000
VERSION     DAFNBJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.065"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..982
                     /locus_tag="L2X49_002473"
     CDS             <1..982
                     /locus_tag="L2X49_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBN4797547.1"
                     /translation="AVLNRFLETDIKEIRIFSRDEKKQDDMRKKYNNSKLKFYIGDVR
                     DYSSILSAARGVDFIYHAAALKQVPSCEFHPLEAVKTNVLGTENVLEAAIANQVKRIV
                     CLSTDKAVYPINAMGISKAMMEKVMVAKSRNVNSDKTVICGTRYGNVMASRGSVIPLF
                     VDLIKAGKALTITDPNMTRFMMTLEDAVDLVLYAFEHGNNGDIFVQKAPAATIETLAI
                     ALKELLNVEQHPVNVIGTRHGEKLYEALLSREEMIAAIDMGDYYRVPPDLRDLNYGKY
                     VEQGDSRISAVEDYNSHNTQRLDVEGMKTLLLKLPFIRALRAGEHYDLDA"
     gene            984..2087
                     /locus_tag="L2X49_002474"
     CDS             984..2087
                     /locus_tag="L2X49_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBN4797548.1"
                     /translation="MKILITGADGFIGRNLCLRLQEAGYCDLVKIDRGSSAADLETGL
                     QDADFVYHLAGINRPKNVDEFAEGNSNLTQQIVDYLLAKHKSIPIMISSSIQAELVNA
                     YGQSKAAAEKHIERYAAESGAAYFIYRYPNVFGKWCKPNYNSFVATFCHNIANNIDIT
                     INDSFAPVNLVYIDDVCSDAIKLLSGKVESGYKTVKPVYSTTVGEVAELLYRFKESRS
                     TLVTEAVGTGFIRALYSTWLSYLPADMFAYSVPSYGDARGVFCEMLKTPSAGQFSFFT
                     AHPGITRGGHYHHTKNEKFLVIRGQACFRFEHVITGERYEMNVSSDEFKIVETVPGWT
                     HDVTNIGADELIVMLWANEIFSRDEPDTIARPL"
     gene            2087..3217
                     /gene="wecB"
                     /locus_tag="L2X49_002475"
     CDS             2087..3217
                     /gene="wecB"
                     /locus_tag="L2X49_002475"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBN4797549.1"
                     /translation="MKKLKIMSVVGTRPEIIRLSRVLAKLDEHCEHILVHTGQNYDYE
                     LNEVFFNDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVLESEKPEAMLVLGDTNSCI
                     SAIPAKRRKVPIFHMEAGNRCFDQRVPEETNRRIVDHTADINMTYSDIAREYLLAEGL
                     PADRIIKTGSPMFEVLTYYMPQIDNSDVLSRLNLRSGEFFVVSAHREENVDSPKQLVK
                     LATILNTIAEKYDLPVIVSTHPRTRNRINEQGIEFHPNINLLKPLGFHDYNHLQKNSR
                     AVLSDSGTITEESSIMNFPAVNIREAHERPEGFEEASVMMVGLDCERVLQALDILATQ
                     PRGETRLLRQVSDYSMPNVSDKVVRIVHSYTDYVKRVVWKEY"
     gene            3217..4428
                     /locus_tag="L2X49_002476"
     CDS             3217..4428
                     /locus_tag="L2X49_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBN4797550.1"
                     /translation="MKLALIIDDYLPNSTRVGAKMFHELAQEFIRRGHDVTVITPDIC
                     LQDDVSFSTFQGVKTWRFKSGPLKDVSKIQRAINETLLSYRAWNSIKSQIKKETFDGV
                     VYYSPSIFWGHLVKKIKSRCQCPAYLILRDMFPQWVIDAGMLKAGSLIERYFRVFERS
                     SYRQANRIGLMSDKNLEVFRVNNKGYPCEVLRNWASLTPTVPPQGYIPLRQRLGLDDK
                     VIFFYGGNIGHAQDMGNLMRLARKMAEHPQAHFLFIGQGDEVELINSLAAEWSLPNFT
                     YLASVNQDEFKFILSEMDIGLFSLSARHSSHNFPGKLLGYMVQSLPILGSVNAGNDLL
                     DVVNQNNAGLIHINGEDDKLHESALLMLKDVAARRQFGLGANALLREQFSVESAAQTI
                     EMRLEACNATH"
     gene            4415..4813
                     /locus_tag="L2X49_002477"
     CDS             4415..4813
                     /locus_tag="L2X49_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HBN4797551.1"
                     /translation="MRLIDTHQLEALYEQAGKSARLRAHLLLHNSHREKVQRLLIALV
                     QGSYVDPHFHELPHQWEMFVVMQGQVQVCLYGKDGEIINQFVAGENTAISVVEFSPGD
                     IHSVECLSPRALMMEVKEGPFDPSFAKAFI"
     gene            4927..6333
                     /gene="gndA"
                     /locus_tag="L2X49_002478"
     CDS             4927..6333
                     /gene="gndA"
                     /locus_tag="L2X49_002478"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBN4797552.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSRDKTEEVIA
                     ENPGKKLVPFYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAET
                     FTEWNKGELNSYLIDITKDIFTKKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAKPAADKAEFVEKVRRALYLGKIVSYA
                     QGFSQLRAASDENNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENAGIANLLLAPY
                     FKKIADEYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLD"
     gene            6496..7833
                     /locus_tag="L2X49_002479"
     CDS             6496..7833
                     /locus_tag="L2X49_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBN4797553.1"
                     /translation="MPLLNDLLDFSDHPFMPPPSAQLFAEHLPVEWIQHCLTLSAHAT
                     VRRRRLPGDMVIWMVVAMAFFRNEPITDVVRRLNLSADGEAGINLLARSAVTQARQRV
                     GAAPVEWLFHQTAQTWGSERYLKDDWHGLQLFAIDGAQFRTPDEPELREYYGSANTST
                     ERQSAYPVMRLVALMNLGSHILVNAVTAPYRRSETVLAHSMLATIPDNSITLFDKLFY
                     SADLLLTLNQQGCNRHWLLPAWKNIAAETEESYGPGDRLLKLKVSLQARKKNPALPEF
                     WYARAVTYEVNGVEKTVLTSLPADRYKAKEVAELYHSRWEIEVGFRNLKSSPLNNALV
                     LRSRKVELLEQEVWGMLLAYNLIRCEATKAAEKHKKAASEISFKFAFQFIATEMIVLG
                     NTASPGTIPKRLEHLRGALEVVFITKRPRPSRPRAVKISKTRYPVKRSTAPLK"
     gene            7970..8428
                     /gene="tnpA"
                     /locus_tag="L2X49_002480"
     CDS             7970..8428
                     /gene="tnpA"
                     /locus_tag="L2X49_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBN4797554.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRATGCILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            complement(8533..9144)
                     /locus_tag="L2X49_002481"
     CDS             complement(8533..9144)
                     /locus_tag="L2X49_002481"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBN4797555.1"
                     /translation="MLTEQQQAQLDWEKTDGLLPVVVQHAVSGEVLMLGYMNQEALAK
                     TVDSGKVTFFSRTKQRLWTKGETSGHFLNVVSITPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGEASHQWLFLYQLEQLLAERKTADPSSSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDREELTNEASDLMYHLLVLLQDQELDLTAVIENLRKRHK"
     gene            complement(9138..9914)
                     /gene="hisF"
                     /locus_tag="L2X49_002482"
     CDS             complement(9138..9914)
                     /gene="hisF"
                     /locus_tag="L2X49_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBN4797556.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVAGGIKSAEDAAKILSFGADKISI
                     NSPALADPELITRLADRFGVQCIVVGIDTWYDATTGKYHVNQYTGDECRTRVTHWETL
                     EWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVRAVCHVPLIASGGAGTMEHFLE
                     AFRDANVDGALAASVFHKQIINIGELKTYLADQGVEIRVC"
     gene            complement(9896..10633)
                     /gene="hisA"
                     /locus_tag="L2X49_002483"
     CDS             complement(9896..10633)
                     /gene="hisA"
                     /locus_tag="L2X49_002483"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBN4797557.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            complement(10633..11223)
                     /gene="hisH"
                     /locus_tag="L2X49_002484"
     CDS             complement(10633..11223)
                     /gene="hisH"
                     /locus_tag="L2X49_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBN4797558.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            complement(11223..12290)
                     /gene="hisB"
                     /locus_tag="L2X49_002485"
     CDS             complement(11223..12290)
                     /gene="hisB"
                     /locus_tag="L2X49_002485"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBN4797559.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELMKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            complement(12290..13360)
                     /gene="hisC"
                     /locus_tag="L2X49_002486"
     CDS             complement(12290..13360)
                     /gene="hisC"
                     /locus_tag="L2X49_002486"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBN4797560.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            complement(13357..14661)
                     /gene="hisD"
                     /locus_tag="L2X49_002487"
     CDS             complement(13357..14661)
                     /gene="hisD"
                     /locus_tag="L2X49_002487"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBN4797561.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            complement(14667..15566)
                     /gene="hisG"
                     /locus_tag="L2X49_002488"
     CDS             complement(14667..15566)
                     /gene="hisG"
                     /locus_tag="L2X49_002488"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBN4797562.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     misc_feature    complement(15613..15736)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(15712..15762)
                     /gene="hisL"
                     /locus_tag="L2X49_002489"
     CDS             complement(15712..15762)
                     /gene="hisL"
                     /locus_tag="L2X49_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBN4797563.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            16045..16296
                     /gene="yefM"
                     /locus_tag="L2X49_002490"
     CDS             16045..16296
                     /gene="yefM"
                     /locus_tag="L2X49_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBN4797564.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            16293..16547
                     /gene="yoeB"
                     /locus_tag="L2X49_002491"
     CDS             16293..16547
                     /gene="yoeB"
                     /locus_tag="L2X49_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBN4797565.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            16630..17454
                     /locus_tag="L2X49_002492"
     CDS             16630..17454
                     /locus_tag="L2X49_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBN4797566.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDVDALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            17500..18429
                     /locus_tag="L2X49_002493"
     CDS             17500..18429
                     /locus_tag="L2X49_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4797567.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            18644..18706
                     /gene="yoeI"
                     /locus_tag="L2X49_002494"
     CDS             18644..18706
                     /gene="yoeI"
                     /locus_tag="L2X49_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBN4797568.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            18696..20054
                     /gene="plaP"
                     /locus_tag="L2X49_002495"
     CDS             18696..20054
                     /gene="plaP"
                     /locus_tag="L2X49_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBN4797569.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            20233..21291
                     /gene="yeeE"
                     /locus_tag="L2X49_002496"
     CDS             20233..21291
                     /gene="yeeE"
                     /locus_tag="L2X49_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBN4797570.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYLAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            21305..21532
                     /locus_tag="L2X49_002497"
     CDS             21305..21532
                     /locus_tag="L2X49_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBN4797571.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(21575..23002)
                     /gene="sbcB"
                     /locus_tag="L2X49_002498"
     CDS             complement(21575..23002)
                     /gene="sbcB"
                     /locus_tag="L2X49_002498"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBN4797572.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISLLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            23211..24377
                     /gene="dacD"
                     /locus_tag="L2X49_002499"
     CDS             23211..24377
                     /gene="dacD"
                     /locus_tag="L2X49_002499"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HBN4797573.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDEENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
BASE COUNT         5889 a         5995 c         6322 g         6254 t
ORIGIN      
        1 cgctgtactt aatcgttttc ttgaaactga tattaaagaa ataaggattt tttctcgcga
       61 tgaaaaaaaa caagacgata tgcggaaaaa atataataat tctaagttaa aattctacat
      121 tggggatgta agggattatt ccagcattct aagcgccgca cgtggcgtcg actttatata
      181 ccatgctgct gcattaaaac aagttccgtc atgtgaattt catcctctgg aagcagtgaa
      241 aactaacgta ttaggtacag agaatgtgct tgaagcagca attgcaaacc aggttaaacg
      301 tatcgtttgc ttaagcacgg ataaggcagt atatccgatt aatgccatgg gtatttccaa
      361 agcaatgatg gaaaaagtga tggtcgcaaa atcacgtaat gttaatagcg acaaaactgt
      421 gatttgcggt actcgctacg gcaacgtgat ggcttcacgt ggttccgtta ttcccttatt
      481 cgttgatctg atcaaagcgg gcaaagcgct gacgatcact gacccaaata tgacccgttt
      541 tatgatgacg cttgaagatg ctgtcgatct ggtcctatat gcgtttgaac atggtaataa
      601 tggcgatata tttgtccaaa aagcgcctgc tgcaacgatt gaaacattag ccattgctct
      661 caaagaactt cttaatgttg agcaacatcc tgttaatgtt atcggaacgc gccatggtga
      721 gaaactttat gaagcgctgc ttagccgtga agagatgatt gccgccattg acatggggga
      781 ttactaccgt gttccaccag acctgcgcga tcttaactat ggaaaatacg tagaacaagg
      841 tgatagccga atctcagcag tagaggatta taactctcac aatacacagc gactggatgt
      901 tgaagggatg aaaacgcttc ttttgaaatt accttttatt cgtgcactgc gtgcgggtga
      961 acattacgat ctggatgcct aatatgaaaa tcctgattac tggagctgat ggttttattg
     1021 gacgtaacct gtgcttacgc cttcaggaag caggctactg tgaccttgtt aagattgacc
     1081 gcggttcaag tgcggctgat ctggaaactg gccttcaaga tgctgatttt gtctatcatc
     1141 tcgcaggtat caatcgacct aaaaacgttg atgaatttgc cgaggggaat agcaatctga
     1201 ctcaacagat tgttgattat cttttagcca agcataaaag catacctatt atgatcagtt
     1261 cttccattca ggctgaactg gttaatgctt atggtcaaag taaagctgca gcagaaaaac
     1321 atattgaacg ctatgcagct gaaagcggtg cagcttattt tatttatcgt tatccgaatg
     1381 tttttggtaa gtggtgtaag cctaattata attcgttcgt ggcgaccttt tgccataata
     1441 ttgccaacaa tatcgatatc acgatcaatg actccttcgc gcctgttaat cttgtttata
     1501 ttgatgatgt ctgttctgat gcgataaagc tcttgtctgg aaaggttgaa agcgggtaca
     1561 aaactgttaa gccagtatat tcgacgacag taggtgaggt ggcggaatta ctttatcgct
     1621 tcaaagaaag ccgttccact cttgtcaccg aggctgtagg aacagggttc atccgcgcgc
     1681 tgtattcgac gtggttaagt tatctcccag ccgatatgtt tgcgtattca gttccctctt
     1741 acggagacgc ccgaggggtt ttttgtgaaa tgttaaaaac cccttcagcg gggcagtttt
     1801 cattttttac agcgcatccc ggcattacac gtgggggtca ttatcatcac accaaaaatg
     1861 agaagttcct ggtcattcgc ggccaggcat gctttaggtt tgaacatgtg attaccggtg
     1921 agcgatatga gatgaatgtt tcctcagatg agttcaaaat cgttgagaca gtccccggct
     1981 ggacacatga tgttacaaat attggagcgg atgaattgat agtcatgctg tgggcaaacg
     2041 aaattttcag tcgcgatgag cctgatacta ttgcgagacc tctgtaatga aaaaactaaa
     2101 aattatgtct gttgttggta cgcgtcccga gattatccgt ctgtcacgcg ttctcgctaa
     2161 gcttgatgaa cactgcgagc atattcttgt ccatactggt caaaactatg attatgagtt
     2221 aaacgaagta ttctttaatg accttggtgt acgaaaaccc gattactttt taaatgctgc
     2281 tgggaaaaat gctgcagaaa ccatcggtca ggttatcatc aaagttgatg aggtacttga
     2341 atccgaaaag cctgaagcaa tgttggtgct gggtgataca aactcctgta tttctgcaat
     2401 tccagccaaa cgccgtaaag tgcctatctt ccatatggaa gctggcaatc gttgtttcga
     2461 tcagcgcgtc ccggaagaga ccaatcggcg cattgtagac cataccgctg acatcaatat
     2521 gacctacagc gatattgcac gtgaatacct tcttgctgaa ggcctcccag cggaccgaat
     2581 tattaaaacc ggtagcccaa tgtttgaggt acttacgtat tatatgcctc aaatcgataa
     2641 ttcggatgta ctgtcgcgtc tgaacctgcg ttcaggcgaa tttttcgtcg tcagcgcgca
     2701 tcgtgaagag aatgttgatt ctcccaaaca gctagtcaag cttgcgacta ttctcaatac
     2761 tattgctgaa aaatatgatt tgccggttat tgtatccact catccgcgga cacgtaatcg
     2821 tattaacgag caagggattg aattccatcc aaatattaat ctactgaaac cgttaggttt
     2881 ccatgattac aaccatttgc agaaaaattc acgtgctgtg ctgtctgaca gcggtacgat
     2941 aactgaagaa tcttccatta tgaatttccc ggcggtaaat attcgggaag cacatgagcg
     3001 tccagagggc tttgaagaag cctctgtcat gatggtgggc ctggattgtg aacgtgttct
     3061 acaggcactg gatattctgg caacacagcc ccgcggcgaa acccgtcttt tacgacaagt
     3121 aagtgactac agcatgccta atgtgtcaga taaagtcgtc agaatcgttc attcttatac
     3181 tgattatgtc aagcgagtcg tctggaaaga atactgatga aacttgcttt aatcatagat
     3241 gattacctgc ccaatagtac gcgagttggt gcaaaaatgt ttcatgaatt ggctcaggaa
     3301 tttattcgcc gtgggcatga tgttacggta attacacctg acatctgtct tcaggatgat
     3361 gtgtccttta gcaccttcca gggggtcaag acatggcgtt tcaaaagtgg gcctctcaag
     3421 gatgtgagca aaattcaacg agccatcaat gaaacacttc tatcctatcg tgcctggaat
     3481 tccattaaaa gccagataaa aaaagagact tttgatgggg tagtttatta ctcaccctcc
     3541 attttctggg ggcacttagt caagaaaatt aaatctcgtt gccagtgtcc ggcttacctg
     3601 attttgaggg atatgttccc gcaatgggtg atagacgctg gcatgttaaa agccggttcc
     3661 ctcatcgaac gctatttccg tgttttcgaa agatcatctt atcgccaggc aaaccgtatc
     3721 gggctgatgt cagataagaa tcttgaggtc tttcgggtta acaacaaagg ttatccttgt
     3781 gaggttttgc gtaactgggc ttctctcacg ccgacggtac caccccaggg ttatatccca
     3841 ttgcgtcagc gtcttggcct tgatgataaa gttattttct tctatggagg gaatatcggt
     3901 catgcgcagg atatggggaa tctgatgcgt cttgctcgaa aaatggctga gcatccgcaa
     3961 gcccattttt tatttatcgg gcagggggat gaagttgaat taatcaattc tttggctgcc
     4021 gagtggtcat tgcccaactt cacgtatttg gcctctgtca atcaggatga atttaaattt
     4081 atcctctcgg aaatggatat aggcttgttt tcactttccg ccagacattc ttcgcataat
     4141 ttcccgggta aattgctagg gtatatggtt cagtccttac ctatactagg cagtgtgaat
     4201 gctggtaacg atttgcttga tgtcgtcaat caaaataatg ccggattaat tcatatcaat
     4261 ggtgaggatg acaagcttca tgagtccgca ctgttaatgc ttaaggatgt tgctgcgcga
     4321 cgtcaattcg gcttaggcgc gaatgcatta ttgagagaac agttctccgt tgagtctgcg
     4381 gcacagacta tagaaatgag gttagaggca tgtaatgcga ctcattgata ctcaccaact
     4441 tgaagcttta tacgaacaag ccggaaaatc tgcacgcttg cgcgctcatc tcttattgca
     4501 caattcgcac cgagagaaag tgcaacgtct gctcattgcc ttggttcagg gcagctatgt
     4561 cgatccgcat ttccatgaac ttcctcatca atgggagatg tttgtcgtta tgcaggggca
     4621 ggttcaagtc tgtttgtacg gcaaagatgg cgaaatcatt aatcaatttg tcgctggaga
     4681 gaatactgca ataagcgtag tcgagttttc tccgggtgat atacacagtg tcgaatgtct
     4741 ctctccgaga gcattaatga tggaagtgaa agaagggcct tttgatcctt cctttgccaa
     4801 ggcgttcatc taacgcccct ctgaatcgca tcttccgcta tctactcagg ctcatcctga
     4861 gttaacatct aagccacatt tcaagccgcg cacagtcgcg gcgaccacac ctgacaggag
     4921 tatgtaatgt ccaagcaaca gatcggcgtt gtcggtatgg cagtgatggg gcgcaacctg
     4981 gcgctcaaca tcgaaagccg tggttatacc gtctccgttt tcaaccgctc ccgtgataag
     5041 accgaagaag tcatcgctga gaatccgggt aagaaactgg ttcctttcta tacggttaaa
     5101 gagtttgttg aatctctgga aacgcctcgt cgtatcctgt taatggtgaa agcgggcgca
     5161 ggtaccgatg cagccatcga ttccctgaaa ccttatcttg acaaaggcga catcatcatt
     5221 gatggtggta acaccttctt ccaggacacc attcgtcgta accgtgaact ctctgctgaa
     5281 ggtttcaact tcatcggtac cggtgtttcc ggtggtgaag agggcgcgct gaaaggccca
     5341 tctatcatgc ctggcggcca gaaagaagcg tacgagcttg ttgcgccaat cctgaccaaa
     5401 atcgctgcgg ttgcggaaga tggtgagccg tgtgtgacct atatcggtcc ggacggtgca
     5461 ggccactatg ttaagatggt tcacaacggc atcgaatacg gcgatatgca gctgattgct
     5521 gaagcctact ctctgctgaa ggggggcctg aacctttcca acgaagaact agcagagacc
     5581 ttcactgagt ggaacaaagg cgagctgaac agctatctga tcgacatcac caaagatatc
     5641 ttcacgaaga aagatgaaga gggtaaatac ctggtcgatg tgattcttga cgaagcggcg
     5701 aacaaaggta ccggcaaatg gaccagccag agctctctgg acctgggcga accgctgtct
     5761 ctgatcactg aatccgtatt cgctcgttac atctcttctc tgaaagatca gcgcgttgcg
     5821 gcttccaaag tgctgtctgg tccgcaggct aaaccagctg ctgacaaagc ggaattcgtt
     5881 gagaaggttc gtcgtgctct gtacctgggc aaaatcgtct cttacgcgca gggtttctct
     5941 cagttacgcg cggcttctga cgaaaacaac tgggatctga actacggtga aatcgcgaag
     6001 atcttccgtg caggttgcat catccgcgcc cagttcctgc agaaaatcac cgatgcctat
     6061 gctgaaaatg caggtatcgc gaacctgctt ctggcacctt actttaagaa gattgctgac
     6121 gaataccagc aggcgctgcg tgatgtggtt gcatacgctg tacagaatgg tattcctgta
     6181 ccgaccttct ccgctgcagt ggcgtattac gacagctatc gcgctgccgt actgcctgcc
     6241 aacctgatcc aggcgcagcg tgactacttc ggtgcgcata cgtataaacg tacggataaa
     6301 gaaggtgtgt tccacaccga atggctggat taatctaatt agctctgcca ttccatcaag
     6361 cccggtagcg ataccgggct tttttgtgca cagaaaaccc ccagctaggc tgcaatgccg
     6421 atcagttaag gatcggttga ccgatcctta aactgcggca ctataacggc ttccacaaca
     6481 gggagccgtt ttcttatgcc acttctcaat gatctgctcg atttcagtga ccatccgttt
     6541 atgccaccgc cctctgcaca actgtttgca gagcaccttc ccgtcgagtg gatacagcac
     6601 tgtctgacgc tttctgcgca tgcgaccgtt cgccgccgtc gtttaccggg ggacatggtt
     6661 atctggatgg tggtagccat ggcctttttc cgcaatgagc caattaccga tgtggttcgc
     6721 cgtctgaacc tgagcgcgga tggcgaagcg gggatcaacc tgctggcccg cagcgctgtc
     6781 acccaggcgc gtcagcgcgt gggggccgcc ccggtggaat ggctcttcca ccagaccgca
     6841 cagacatggg gctcggaacg ttacctgaag gatgactggc acggcctgca gctttttgcc
     6901 attgatggcg cacagttcag gacacctgat gaacctgagt tgcgtgaata ttatggctct
     6961 gccaacacat ccactgagcg gcagagcgcc tacccggtca tgcgtctggt ggcgttgatg
     7021 aatctgggaa gccacatttt ggtgaatgcc gtgaccgcgc cttaccggcg gagcgaaacc
     7081 gtgctggctc actccatgct cgccactatc ccggataact ccattacgct gtttgacaag
     7141 ctcttttaca gcgcagacct gctgctgacg ctgaaccagc agggttgtaa ccgccactgg
     7201 ctactgcctg cgtggaagaa tatcgcggca gaaacagaag aaagttacgg tcccggagac
     7261 aggcttctga aactgaaggt gtcgctgcag gcgaggaaaa agaatcctgc actgccagag
     7321 ttctggtacg cccgggcggt gacttatgag gtgaacggtg tggagaaaac ggtgctgaca
     7381 tcactcccgg cagaccgcta caaagcaaaa gaggtagcgg aactttacca ttcacgatgg
     7441 gaaatcgaag tcgggttcag aaacctgaaa agcagcccgc tgaacaatgc cctggtgctg
     7501 agaagccgta aggttgagct gctggaacag gaggtgtggg gcatgttgct ggcttacaac
     7561 ctgatacggt gtgaggcaac aaaagcggct gagaaacaca aaaaagcggc gtcagaaatc
     7621 agctttaagt tcgcgttcca gtttatcgcg acagaaatga tagtgctggg aaatacggca
     7681 tcaccgggga ccatcccgaa acgactggag catctgcggg gtgctctgga agttgtgttc
     7741 ataacaaaac gcccccgacc atcaaggccg agggcagtta agatatcaaa aacccgttat
     7801 ccggtgaaac gcagcaccgc tccgcttaag tgaacggcat tgcagctagg ctgggggttc
     7861 cggaaagctt tcagctttga gccagttatt aaaacccctt ttgatttgtt aaaacacctt
     7921 gcggtctggc aactgcaagt gtcaaacaag aaatcaaaag ggggtcccaa tggggaacga
     7981 aaagagctta gcgcacaccc gatggaactg taaatatcac atagtatttg cgccaaaata
     8041 ccgaagacag gtgttctaca gagagaagcg tagagcaaca ggctgtattt tgagaaagct
     8101 gtgtgagtgg aaaagtgtac ggattctgga agctgaatgc tgtgcagatc atatccatat
     8161 gcttgtggag atcccgccca aaatgagcgt atccggcttt atgggatatc tgaaagggaa
     8221 aagcagtctg atgctttacg agcagtttgg tgatttgaaa ttcaaataca ggaacaggga
     8281 gttctggtgc agagggtatt acgtcgatac ggtgggtaag aacacggcga agatacagga
     8341 ttacataaag caccagcttg aagaggataa aatgggagag cagttatcga ttccctatcc
     8401 gggcagcccg tttacgggcc gtaagtaacg aagttggatg caaatgtcag atcgtgtgcg
     8461 cctgttaggg cgcggctggt aagagagcct tataggcgca tttgaaaaac ctccggctat
     8521 gccggaggat atttatttat gccgcttacg aagattctca atcaccgccg tcaaatccag
     8581 ctcttgatcc tgcagcagca ccagtaggtg atacattaaa tcagacgctt cattggtcag
     8641 ctcttcgcgg tcatgtaccg tcgcggccag cgcggtctca acgccttctt cacctacttt
     8701 ctgcgcaata cgcttggtgc cgcttgcata cagcttcgcc gtatacgagc tggaaggatc
     8761 tgctgtttta cgctcagcca gtaactgctc cagttgatac aggaacagcc actggtggct
     8821 agcctctccg aagcaactgc tggtgccttt atggcaggtc ggaccgatgg ggttagccag
     8881 aaccagcaga gtatcgttgt cgcaatccgg cgtaatgcta accacattca ggaaatggcc
     8941 ggaggtttcc cctttggtcc acaggcgctg tttcgtgcgc gagaagaagg tcactttgcc
     9001 gctgtcgacc gttttcgcca gcgcctcctg gttcatgtat cccagcatca gcacttcacc
     9061 ggaaacggca tgctggacaa ccacggggag taatccgtca gttttttccc agtccagctg
     9121 cgcctgttgt tgctctgtta acataccctg atctccacgc cctggtcggc caggtacgtt
     9181 tttaactcac caatattgat aatctgtttg tggaacacgg aagccgccag cgcgccgtca
     9241 acgttcgcat cgcggaaggc ttctaggaag tgttccatag tgcccgcacc gccggaggcg
     9301 atcagcggta cgtgacaaac cgcacgcact tttttcagct gctcgaggtc atacccgtta
     9361 cgcacgccgt cctggttcat catattcagc acaatttcgc ccgcaccacg tttctgcact
     9421 tcctggaccc actccagcgt ctcccaatgg gttacgcggg tacggcattc gtcgccggta
     9481 tactggttga cgtgatactt gccggtggtc gcgtcatacc aggtgtcgat cccgacaaca
     9541 atacactgca cgccaaagcg atcggccaga cgggtgatca gctccgggtc ggccagcgca
     9601 ggggagttga tcgaaatttt gtcggctcca aaggagagga ttttcgccgc atcttctgca
     9661 gacttaatcc cgcccgccac gcagaacgga atatcaatca cttctgcgac gcgcgcgacc
     9721 cagcttttat ccaccacacg gccatcgctg gaagcggtga tatcatagaa caccagttcg
     9781 tctgcacctt cttcagcgta gcgttttgcc agcggcacga tatcgccaat gatttcatgg
     9841 ttgcgaaact gtacgccttt caccacctga ccatcacgaa cgtcgagaca tgggattatg
     9901 cgttttgcca gcatgcgatg gcctccttca cggtgaattt accttccagt aatgcccgac
     9961 caactattac gccgcgcacg ccagtaccac gcagggccgc cacatcatca atgtcgccaa
    10021 taccgccgga agactgaaat gccacctgcg ggtatctggc gcacacttct tcatataaag
    10081 agacgttaga acctgccagc gtgccatcgc gcgagatatc ggtacacagc acatgtttca
    10141 ggccgacggg cagataggtt tccaccagtt gttccagtga aacgcccgag ttctcttgcc
    10201 agccgctgac cgccacctgc ttgttgcctt gctcgtcaat acggacatcc agcgccagca
    10261 ctaaggcatc ggcaccgaaa cgttcaaacc agcctttcac cctttctggt gatttcaccg
    10321 cggtggagcc aaccactacg cgcgcaacgc ccgcttccag taacgccgcc acgtcttctt
    10381 cgctacgcac accgccgcca acctgcaccg gaacgttaac gcccgcgacc agggttttaa
    10441 tcagcgggat ttgacgtttt gccggatctt ttgccccggt cagatccacc agatgcaaca
    10501 cttcggcacc ttgcgcagcg taatcctgta agcgtggcag cggatcgtta ccgtaatcgc
    10561 gttgtttgcc gtaatcgccc tgatggagac gcaccacagt gccgtcaatt aaatctaatg
    10621 ccggaataat catcacatct ccaggaagtt tttcagcaac ttagcgccag cggcaccaga
    10681 acgctccggg tggaactgca cgccgtagaa gttatctttt tgtaccgccg cggtgaacgg
    10741 ttcgccgtaa ttacactggg cgatggtcca cggattgact ggcattgcgt agctgtgaac
    10801 aaagtaaaag tacgcgccgt cttcaattcc ctgaaacagg cggttgcctg cctgcgggta
    10861 aacgcggttc cagcccatat gtggcagcgg cagaccaaag tcggtcattt tcggcacgtc
    10921 ttcgtcgatg atgcccagca aatcgacgcc gttgctctct tcactgcgcc gccccagcag
    10981 ttgcatccct aagcagatgc ccagcaccgg ttgggtacag gctttgatga gatcaaacag
    11041 ctcgcgctca cgcacctgat ccatcgctgc ttgcgcagtg ccaacgccgg gtaaaaatag
    11101 tttatcggcc agcaacacga cgtccgggtc acggctgact ttgggttcat aaccgtgacg
    11161 cgcaatggca gatttcaccg agttcaaatt ggcgcagccg gtatcaagga tcaccacgtt
    11221 cattacagca ctcctttcga cgagggcagg gtgtcgcctt ccacgcggat ggcctggcgc
    11281 agggtgcgac caaaggcttt gaacaggctc tctacacggt ggtgatcgtt tttacctttg
    11341 gttttcaggt gcaacgtcac gcccatggtg taagagagcg aacggaagaa gtgctcgatc
    11401 atctcggtgc tgagatcgcc cacccgctgg taggtaaatt cggctttata ttccaggtgc
    11461 gggcgaccag agatatccag tgcacagcgg gcaaggcatt cgtccattgg cagcacaaaa
    11521 ccgaagcggc aaataccgcg tttgtctccg agggcgattt ttagcgcttc gcccagcgcc
    11581 agaccggtat cttcgacggt gtggtgatcg tcgatataga ggtcgccttt gacgttgatt
    11641 tccatgcgga aaccgccgtg ggtagcgatc tgatccagca tgtgatcaaa gaagccaaca
    11701 ccggtgttaa tcttgctgcc accttcgcga tccagccaca cctggacgtc aatttgcgtc
    11761 tctttggtgt tgcgcactac atgggcgtaa cggtctcgtt tagtgagttg ctcgccaatc
    11821 atcggccagt tcagggtttc gcggtcatag cgtaaaccat taatgcccat gttttccgcc
    11881 agttgaatgt cggtcgcgcg atcgccaatc acataactgt tagcgcgatc catcgcttgc
    11941 tcagccagat aacgctccac cagttttact ttcggcttac ggcagtcgca ctcatcggcg
    12001 ggcaggtgcg gacaaatcag cacttcatca aactgcacgc cttgcgaggt gaagatctgc
    12061 atcatcaggt tgtgcgggcc atcaaaatcc gcctgcggga aactttgtgt tcccagacca
    12121 tcctgattag tgatcatcac cagcttgtag cccgcttttt gcagcttcat cagctccggg
    12181 atcacgcccg gttcaaaggc gagtttatca aaacggtcca cctgaaaatc actcggcggt
    12241 tcgctaatca gggttccatc gcgatcgata aaaagatact tctgactcat caaacttgct
    12301 ccgcacgtaa ggcgtcaatg acgcgctggc tttcttcacg ggttccgacg gtaattcgca
    12361 ggcagccgct taaagagggt tgtttattct gatcacgtaa gataatgccc tgatcccaca
    12421 aagatttaaa cactgcgctg gaggctttaa agcgcgccag aatgtagttg gtttcagagt
    12481 cgaaaacctg ctccacgcag gggatctctt tcagtgcggc aatcaggtat tcgcgttctg
    12541 caataatttg cgctacccgt tcgcgcatgg cgacgattcc ctgcgggctt aacgcctgtg
    12601 cggcaatgtc ggcaaccggc gtcgagagcg ggtagggggc gatcactttc atcagcaggt
    12661 tgatgacttc ttcgtttgcc agcgtaaatc cgcaacgaag ccccgccaga gcaaaagctt
    12721 tcgacagtgt gcgtaaaata gccaggtgcg gatattccgc cagccagcca gccagcgatg
    12781 cctgcgggca aaactcgata taggcttcat cggcaaccac aatcgcttta ccgcgggtta
    12841 actccagcag agtgcgaaaa tcctgcgggt tgatcagttg cccggtcgga ttgttggggc
    12901 tgcaaacata gaccactttt acgccgtcca gcttgtcgga aatgccctgt aagtccagtt
    12961 gccagttgtc cagcgtcggc actgtgcggc actcgacgcc aatcgtttcg gcgctgacgc
    13021 tgtacatgcc gtacgttggc gggcagtaga ggatggcgtc tttacctggt tcgcaaaagg
    13081 cgcgaatcag cagttcaata ccttcgtccg cgccacggct gaccagcacc tgctccggtt
    13141 ttacgcctgc atactgcgcg taattttcaa tcaccgcttt cggctgacat tccgggtagc
    13201 ggttgagcgt ttgctgagtg agctgaaact ccacggctgt ggggtattcg ttggcgttca
    13261 gccagacgtc gccgttaccg cccagacgac gcgccgactg atacggcgtc aggttgcgga
    13321 cgttttcacg cgctaaatcg gtaatagtca cggtgctcat gcttgctcct taagggcgtt
    13381 aacacgcaaa gtaacggcat ttttgtgggc ggtcaggcgc tcggcggcgg ccagtgtttc
    13441 aatggtcgaa gccagcgcgg agaaacctac ttttgatagt tcctgcacgg tcatgcgctt
    13501 ctggaaatcc gccagcccga ggctggaaca ggtggcggtg taaccgtaag tcggcagaac
    13561 gtggttggtg ccggaggcgt aatcacctgc cgattccggt gaccagtcac caagaaatac
    13621 cgaaccggcg ctggtaatgc tatcgaccag ttcacgggcg ttgcgggtct gaatgatcag
    13681 gtgctccggg ccgtactggt tggagatctc tacgcactgc gctaaatctt tagtcacgat
    13741 caggcggctg gcgtttagtg cctgacgtgc ggtttcggca cgcggcagtt ctgccagctg
    13801 gcgttcgaca gcttcggcaa cttgatgcgc catatcagca tcgggcgtca gtaaaatcac
    13861 ctgtgagtcc gggccgtgtt cagcctggga aagcaaatca gaagccacga aatccggcgt
    13921 agcgccgctg tcagcaatca ccagcacttc cgacgggcct gcgggcatat cgatcgccgc
    13981 accgtccaga cgctggctca cctggcgttt tgcctcggtg acaaaggcgt tacccggccc
    14041 gaagattttg tccactttcg gcacagattc agtaccaaac gccagtgcgg caatggcctg
    14101 tgcgccgccg acgttaaaca cgtcctgtac accgcacagc tgcgccgcat aaaggatctc
    14161 atcagcaatc ggcggcggtg agcacagcac cactttttta cagcccgcaa tacgcgccgg
    14221 agttgccagc attaataccg ttgagaagag cggggcggag ccgccaggaa tatacaaccc
    14281 aactgaagcg accgggcgcg tgacttgctg gcaacgtacg cctggctgcg tttctacatc
    14341 taccggcggc agcttttgcg cagtgtggaa ggtttcaata ttttttactg ccaccgccat
    14401 cgcctgtttt agctcgtcgc tcaggcgttc gctggcggcg gcgatctcct cagcagacac
    14461 cttcagtgcg gtaaccgtgg ttttatcaaa cttcgcgctg tattcccgca gggcgtcatc
    14521 accacgtgct ttcacgttat cgagaatatc gttaaccgtg cgggtaatgc tttcagaagc
    14581 ggagatcgcc gggcgcatta acagctggcg ttgttgctcc gcagtacagc tattccagtc
    14641 aatgattgtg ttaaagctca tggcgatcac tccatcatct tctcaatcgg caggaccaga
    14701 attgaactgg cacccagcgc tttcagtttt tccatcgttt cccagaacag ggtttcgcta
    14761 ctgaccatgt gcatcgctac gcgttgttgg tcacccgcca gcggcagaat ggttgggcgt
    14821 tcggcacctg gcagcagggc gatgacttca tccagacgtt cggtcggtgc gtgcatcatg
    14881 atgtattttg attcgcgcgc ctggatcaca ccctgaatac gggtcagcag tttgtcgatc
    14941 agctgctgtt tggattcttc catttcacca tcgcgctgga taaggcaggc tttcgagcgg
    15001 tagataactt cgacttcgcg caggccgttt gcttccagcg tggcaccggt ggaaaccaga
    15061 tcgcaaatcg catccgccag tccggcacgc ggggcgactt caacagaacc gttcagtaag
    15121 caggatttaa aagagatgcc tttctggtcg agataacgct tgagcaggtg aggataagag
    15181 gtggcgatac gtttaccgtt taaggagagc gggccgtccc aggcttcatc aaccggcgtt
    15241 gccagcgaaa ggcggcagcc gccgaaatcc agacgacgca aggtaaagta gcgcggatct
    15301 tcaccctggg cgcggcggtt aagcagctct tcttccagca cgttttcgcc aataatcccg
    15361 aggtcaacca cgccatccat taccagaccg ggaatgtcgt cgtcacgcac gcgcagaata
    15421 tcaatcggca tgttttctgc catcgcgatc aggcgctggg tgtgaagatt gattttaatg
    15481 ccacagcgcg ccagcaattc gcgtgagtca tcacttaaac ggccggattt ctgcatagct
    15541 atgcgtaaac gagtgttgtc tgtcattttg ttattcctct ttaaacctgt ctgaaccgtt
    15601 atcaaccgcg caataaaaaa gcccccggaa ggtgatcttc cgggggcttt ctcatgcatt
    15661 catgcaccac tggaagatct gaatgtcttc cagcacacat cgcctgaaag actagtcagg
    15721 atgatggtga tgatggtggt gtttaaattg aacgcgtgtc ataaaagtct ctgtgaatgt
    15781 ttattcaact gatgtctttt aacctaaacc actttcacgt tagaaagcaa gggctttttt
    15841 atatatttta tggcgatgga tttgtttaat gaatgagcgg ggaaaggagg gggctaagag
    15901 gactgtcctc taagctgacg gcaccaaaaa tagcgaagcc tggattttcg tctcactgac
    15961 aataccttga tctacaaact aattaataaa tagttaatta acgcccatca ttgtacaatg
    16021 aactgtacaa aagaggagat tgacatgcgt acaattagct acagcgaagc gcgtcagaat
    16081 ttgtcggcaa caatgatgaa agccgttgaa gatcatgccc cgatccttat tactcgtcag
    16141 aatggagagg cttgtgttct gatgtcactc gaagaataca actcgctgga agagacggct
    16201 tatctactgc gttcccccgc taacgcccgg agattgatgg actcaatcga tagcctgaaa
    16261 tcaggcaaag gaacagaaaa ggacattatt gagtgaaact aatctggtct gaggaatcat
    16321 gggacgatta tctgtactgg caggaaacag ataagcgaat tgttaaaaag atcaatgaac
    16381 ttatcaaaga tacccgcaga acgccatttg aaggtaaggg gaagccagaa cccctgaaac
    16441 ataatttgtc aggtttctgg tcccgacgca ttacagagga gcaccgtctg gtatacgcgg
    16501 ttaccgacga ttcactgctc attgcagcat gtcgttatca ttattgaaca gattcattca
    16561 tagaatgaat tgtcagtttt gatacgctag cgtagcctta tagatctaag gtgtatcagg
    16621 agataacgga tgaaaaaggt cgcaattgtc gggttagggt ggttaggcat gccgctggcg
    16681 atgtcacttt cagcgcgagg ctggcaagtc accgggagta aaaccacaca agatggtgtc
    16741 gaagcggccc gaatgagtgg cattgatagc tatctgcttc gcatggagcc tgagttagtt
    16801 tgcgattctg acgatctgga tgccctgatg gatgtcgatg cgctggtcat tacgcttccg
    16861 gcacgtcgta gcggccccgg cgatgagttc tatttacaag cggtacaaga gttagtggat
    16921 agcgcgctgg cccatcgtat tccccgcatt atttttacca gctcaacatc tgtctatggc
    16981 gacgcgcaag gcacggtgaa agaaactacc ccgcgtaatc cggtaaccaa cagtggacga
    17041 gtattagaag aactcgaaga ctggctgcac aatttacccg gtacttcggt cgatattctg
    17101 cgtcttgcgg ggctggtcgg accgggacgt catcccggac gcttctttgc cggaaaaacc
    17161 gcgcctgatg gtgaacatgg tgttaattta gtccatttag aagatgttat tggcgctatc
    17221 actctgttgt tacaggcacc taaaggcgga cacatctata atatatgtgc gccagctcac
    17281 cctgcgcgta atgttttcta tccgcagatg gcccgtttac tggggctgga gcctccgcag
    17341 ttcagaaata gtctggacag cggcaaaggt aagattattg atggcagtcg gatttgtaat
    17401 gaactgggat ttgaatacca gtatcccgat ccgctggtaa tgccgctgga gtaaacgatc
    17461 acgccagcag gacgccgcat gcactgcaag ggggcgtgta tgaaaccact gctggatgtg
    17521 ttgatgatcc tcgatgcctt agaaaaagaa ggcagttttg cggcggcgtc ggccaaactc
    17581 tataagacgc cgtccgctct gagttacacc gttcacaagc tggaaagcga ccttaatatt
    17641 caattgcttg atcgtagcgg tcaccgcgct aaattcaccc gtaccggaaa aatgttatta
    17701 gagaaagggc gggaagttct gcataccgtg cgagaactgg aaaagcaggc gatcaaactg
    17761 catgaaggct gggaaaacga gctggtgatt ggcgtggacg acacgtttcc tttttctctg
    17821 cttgcgccac tgattgaagc tttttatcaa catcatagcg tgacgcgcct gaagtttatc
    17881 aatggcgtac tcggtggttc ctgggatgcc ctgactcagg ggcgagcgga tattattgtt
    17941 ggcgcgatgc atgagccacc atcttccagt gagtttggtt tttcacggtt gggcgatctg
    18001 gaacaagtct ttgcggtcgc gcctcatcat ccgttagctc tggaagaaga gccgttaaac
    18061 cgtcgtatta ttaagcgata tcgggcgatt gtggtggggg atactgcgca ggcaggcgct
    18121 tctacggcct cacaacttct cgacgaacaa gaagccatta ccgttttcga ttttaaaacc
    18181 aagctggagc tgcaaattag cggcctcggc tgcggctatt taccccgtta tctggcgcaa
    18241 cggtttctcg atagtggcgc gttaatcgag aagaaagtgg tcgcccaaac tctctttgaa
    18301 cccgtctgga ttggctggaa tgaacagacc gcagggcttg ccagcggctg gtggcgggat
    18361 gaaattttag caaatagtgc gatcgccggt gtttatgcaa aatctgatga cggaaaatca
    18421 gccatttaaa gaaaaattat tatgacaagc ctctcattct cttgtcattt cccccccatt
    18481 taggcacaat gcgccgctgt caaaaaatga ctaaaaaccg acgtttcatc agcgtcggtt
    18541 attttttgct tcaaaccaat cattcatacc aagaggccgg gcttcgtacc ggatagatat
    18601 ttactaaaaa tcgacagttg ttgtcgctga ggaatccaaa aaaatggggc aattttttgc
    18661 ttacgcgacg gttatcaccg taaaggagaa tgaccatgtc gcataacgtt actccaaaca
    18721 cctctcgcgt ggaattgcgt aaaacgctta cgttagttcc ggttgtaatg atgggtcttg
    18781 cctatatgca gccgatgacg ctgtttgata cttttggtat cgtttcaggc ctcacggatg
    18841 gtcatgtgcc gacagcctat gcgttcgcat tgattgcgat cctgtttacg gctctgagct
    18901 acgggaagct ggttcgccgc tatccttctg ctggctctgc atacacttac gcccagaaat
    18961 ccattagccc gactgtcggc tttatggtgg gttggtcttc tctgctcgac tatctgttcg
    19021 cgccgatgat caacattctg ctggcgaaaa tctattttga agctctagtg ccttccatcc
    19081 catcgtggat gtttgtggtg gcgctggtgg cctttatgac cgcctttaac ctgcgtagtc
    19141 tgaaatccgt agcgaacttc aacactgtaa tcgtcgtgtt gcaggtagtg ctgatcgcgg
    19201 tgattctggg catggttgtt tatggcgtat ttgaaggtga aggcgctggt acgctggcga
    19261 gcactcgtcc gttctggtct ggcgatgcgc atgttatccc gatgattacc ggggcgacaa
    19321 tcctgtgctt ctcctttacc ggctttgacg gcatcagcaa cctgtcggaa gaaaccaaag
    19381 atgcagagcg cgtgatcccg cgtgcgattt tcctgaccgc gctgattggc ggcatgatct
    19441 tcatctttgc aacttacttc ctgcagctgt acttcccgga tatctctcgc tttaaagatc
    19501 cggatgcgtc acagcctgaa atcatgctgt acgttgcggg taaagcgttc caggttggcg
    19561 cgctgatctt ctccaccatt accgtactgg cgtccggtat ggcggcgcat gcaggcgtag
    19621 cgcgtctgat gtacgtaatg ggtcgtgacg gcgtattccc gaaaagcttc ttcggttatg
    19681 tacacccgaa atggcgtact ccggcgatga acatcatcct ggttggcgcg attgccctgc
    19741 tggcaatcaa cttcgacctg gtaatggcta cagcgctgat taactttggt gcgttggtgg
    19801 cgttcacctt cgttaacctg tcggtcatct cgcagttctg gatccgtgag aagcgtaata
    19861 agacgctgaa agatcacttc cagtatctgt tcctgccgat gtgtggtgcg ctgaccgttg
    19921 gtgcgctgtg ggttaacctg gaagaaagct caatggttct gggtctgatc tgggcggcta
    19981 tcggtctgat ttacctggct tgcgtcacca agagtttccg taacccggtt ccgcagtacg
    20041 aagacgtagc gtaatcatct tttgtaataa taaaccggag tcgaatgaat atttggctcc
    20101 ggttttttta ttctttttta tcgacatcct cattaataac catttgttct aatttagaga
    20161 atttggttat tggtctgcat tccaccagtc atgaataatt tccctattaa cattcatctg
    20221 gttaattaat ttatgttttc aatgatatta agcgggctaa tttgtggtgc tctgctggga
    20281 tttgtcatgc agcgtgggcg tttttgcctg acaggtggtt ttcgcgatat gtatctcgcg
    20341 aaaaataatc gcatgtttta cgccttgctg attgcaattt cggtacaaag tgtgggggtt
    20401 tttgcgttaa ttcaggcggg cctactgact tacgaagccg gggcgttccc gtggcttggt
    20461 actgttatag gtgggtatat ctttgggctg ggaattgttc tggcgggtgg atgtgccacc
    20521 gggacctggt atcgcgcggg tgagggattg atcggcagtt ggatcgcgct tttcacttat
    20581 atggtgatga gtgcggtgat gcgttctcca catgccagtg gtttaaatca aaccttgcag
    20641 cactacagta ctgaacataa ctctattgct gagactttta atttgtctgt gtggccgttg
    20701 gttgccgttt tgctggtgat aacgctctgg gtggtgatga aagagttgaa gaagccaaaa
    20761 ctcaaagtcg cgaccttacc gccgcgccga accgggatcg ctcatattct gtttgaaaaa
    20821 cgctggcatc cctttgtcac tgctgtactc atcggtttga ttgcgctttt agcctggccc
    20881 ctgagtgaag caaccggacg catgtttggg ttaggaatca cttccccaac ggccaatatt
    20941 ctgcaatttc tggtcgcggg tgacgtgaaa tacattaact ggggcgtttt cctggtgtta
    21001 gggatcttcg tggggtcatt tattgcagcc aaagcgagcc gtgagttccg cgttcgcgca
    21061 gctgatgcac aaacaacatt acgtagcggg ttaggtggtg tactgatggg cttcggtgcc
    21121 agtattgcag gtggttgctc tatcggtaat ggactggtta tgactgcaat gatgacctgg
    21181 cagggctgga ttggccttgt atttatgatt ctcggagtct ggactgcgtc ttggcttgtg
    21241 tatgttcgac cgcagcgtaa ggcgcgactg gcaactgctg cggcaaatta agtaaggtgt
    21301 aaagatggcg attaaaaagc tggatgtggt gacgcaggtc tgtccgtttc cactcataga
    21361 agcaaaagca gcactggcag aaatggccag cggcgatgag ttggtaattg agtttgattg
    21421 cacccaggca actgaagcaa tcccacagtg ggcggcagaa gaaggacatg ccatcaccga
    21481 ttatcagcag attggcgatg cagcgtggag catcaccgtt caaaaagcct gactcaacat
    21541 tgtcctccgc cgtaccagcg gcggaggctt caaattagac gatctcttcc gcgtactgcc
    21601 aaagcgcttt taacagcgcc actttctctt tgtcatcggc atattgttgt gccagcatct
    21661 gcaattcatc tgcataaccc tgtaaaaact ctggcgtgaa gacctggcgg cggtgctcca
    21721 gccagcgttg ctgctcggca taatccagcg tccccgggaa gttgcgcgcc cgataattga
    21781 acaacagctt ttcaatccgt ttatcgacaa aagtgatatc cagtgccggt aaattacgcg
    21841 gctcggtttc cagcacaatt ttcattgctg cacgatcggc gtcactgaaa aagccgttat
    21901 aaagctgtgc atccacgtta tctgaaggcg taaacggttc ggcttccgcg aatatcgcca
    21961 ccactttttc gcgcacttgc ggattttcac gcagaatttt caggttatcg aggcaatgct
    22021 gacgattaat tcccagtcgg tcggcatctt ccgggcgtag cgtattcgcc tgggccagca
    22081 ccggacattt attgatatgc accagcttaa ccggaacggc ggcgttatcg ccaagatcgg
    22141 tttttgcggt atataaacgc tcgcgcaatg tgtcgctatc cagttccagt aatagcgaaa
    22201 tgtctcctgc caaatccacc ataattacgg cattgcgatt ttcaggatgc cacgccagcg
    22261 gtgccaccca gctggtattg ccgcgccatg ctccaaacat tccggaaacg tgcaccaggg
    22321 gtttcatctg cggaacatca atcaacgcca tcagtttgtg tttattacga tgggtaaaga
    22381 gataatcaaa caggcgtggc tgacgcgttt ttaccagctt tgccatcgca atagtggcgt
    22441 acacatcagc catcgcatcg tgggcgttgc tatgttcaat accattcgct ttggttaaat
    22501 gctcaaggcg aaagctcggt agaccgtcat cattttcagg ccagtttatt ccttccgggc
    22561 gcagggcata acaggcacgc ataacatcca acaaatccca gcgcgagtta tcatgctgcc
    22621 agctccaggc gtaaggatcg tagaaattac gataaaaaac gttgcgtgtg acttcgtcgt
    22681 cgaaacgcac attgttgtag cccagaatac aggtcttcgg tacggtaaaa agcgagtgaa
    22741 tacgggcggc aaacgcggct tcgttttctc ctttcgcccg tgcttcctgc ggggtaatac
    22801 cggtaattaa tacggctcct ggctggggta aatagtcatc agcgggcttg cagtaaaaga
    22861 cttcgggttc gccgatgaca ttgaattcgc tatcggtgcg aatggctgcg aactgtgcag
    22921 ggcgatctaa cgcggggtgc gtgccaaagg tttcgtaatc gtgaaacaaa aaggtagatt
    22981 gttgcttacc gtcattcatc attaggttaa atccgttatt tctgctgtct gccagagtat
    23041 caaatatcac cgtgctaatc agctttagcg cgacaatttg acagcgagta gcaacagatc
    23101 atgtcagatg aaaatgagag gatagtcaca ttttcttgca ctttattcca gccagttcat
    23161 aagtacttcc gtaaaaagaa cagctatttg aaactcctga gggtttgctg ttgaaacgcc
    23221 gtcttattat tgctgcttct ttgttcgttt ttaacttatc gtctggtttt gcggcggaaa
    23281 acattccttt ttcacctcag cctccagaga ttcatgccgg gtcctgggta ctgatggatt
    23341 acaccaccgg acagatcctc accgcgggta atgagcatca acagcgcaat cccgccagcc
    23401 tgacaaagct gatgacgggc tatgtcgtgg atcgcgctat cgatagtcat cgcattacgc
    23461 cagacgatat tgtcaccgtg gggcgcgatg cgtgggcgaa agataatccg gtgtttgtcg
    23521 gttcttcact gatgtttttg aaagagggcg atcgcgtatc ggtacgtgat ttaagccgtg
    23581 gtttaattgt ggattccgga aatgacgctt gtgttgctct ggctgactat attgccggtg
    23641 ggcaacggca gtttgttgaa atgatgaaca actatgccga gaagctgcat ctcaaggata
    23701 cgcattttga aacagtgcat ggtctggatg cacctggcca gcatagctcg gcttatgatt
    23761 tagctgtgct ttctcgtgct atcatccacg gcgagcccga gttttatcat atgtacagtg
    23821 agaaaagtct cacctggaac ggtatcaccc agcaaaaccg taacgggttg ttgtgggata
    23881 aaaccatgaa tgttgacggc ctgaaaacgg gtcatacttc tggtgccggg tttaatctca
    23941 ttgcttcggc tgtagatggg cagcgtcgtc tcattgcagt ggtaatgggg gctgacagtg
    24001 caaaaggtcg tgaggaagag gcaagaaaat tactgcgttg ggggcaacaa aactttacta
    24061 cggtgcaaat tttgcaccgt gggaaaaagg tcggtacgga acgcatatgg tatggcgatg
    24121 aagaaaatat cgccctggga acagaacaag agttctggat ggtactaccg aaagccgaaa
    24181 ttccacatat caaagccaaa tatacccttg atggtaaaga actcaccgcg ccaattagcg
    24241 cccatcagcg ggtaggggaa attgaacttt acgaccgtga taaacaggtg gcgcactggc
    24301 cgctggttac cctggaatct gtcggggaag gcagcatgtt ttctcgcctt agtgattatt
    24361 tccaccataa ggcctgacct ttcttttgca gcagactggc aggagtgcga gtctgctcgc
    24421 ataatcaaca ctcattcctt gtggttttaa tattgcaact
//
LOCUS       DAFNBJ010000066        24439 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000066 DAFNBJ010000000
VERSION     DAFNBJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.066"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1136..1681
                     /gene="cobU"
                     /locus_tag="L2X49_002500"
     CDS             1136..1681
                     /gene="cobU"
                     /locus_tag="L2X49_002500"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBN4797574.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            1678..2421
                     /gene="cobS"
                     /locus_tag="L2X49_002501"
     CDS             1678..2421
                     /gene="cobS"
                     /locus_tag="L2X49_002501"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBN4797575.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGESILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            2433..3512
                     /gene="cobT"
                     /locus_tag="L2X49_002502"
     CDS             2433..3512
                     /gene="cobT"
                     /locus_tag="L2X49_002502"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBN4797576.1"
                     /translation="MQILADLLNTIPAIDSAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AEQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     QELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGIDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNC"
     gene            3574..4509
                     /gene="ldtA"
                     /locus_tag="L2X49_002503"
     CDS             3574..4509
                     /gene="ldtA"
                     /locus_tag="L2X49_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBN4797577.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4566..4641)
                     /locus_tag="L2X49_002504"
     tRNA            complement(4566..4641)
                     /locus_tag="L2X49_002504"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4606..4608),aa:Asn,seq:gtt)
     gene            4968..5885
                     /gene="nac"
                     /locus_tag="L2X49_002505"
     CDS             4968..5885
                     /gene="nac"
                     /locus_tag="L2X49_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBN4797578.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSRA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            5987..6937
                     /gene="cbl"
                     /locus_tag="L2X49_002506"
     CDS             5987..6937
                     /gene="cbl"
                     /locus_tag="L2X49_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBN4797579.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRRGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGAQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            7768..9441
                     /locus_tag="L2X49_002507"
     CDS             7768..9441
                     /locus_tag="L2X49_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBN4797580.1"
                     /translation="MLQNNPDLQSAIKRLWDKFWSSGISNPLTAIEQITYLLFMKRLD
                     ELDHKRQDEGETSGEKYISKFAGTWIPPEERNRPVAEQHPIDKRTLRWSEFKKLQPEE
                     MLQHVRDKVFPFLKDLNGAESNFTHHMKNAVFIIPKSALLVEAVKAIDEIFEMMKRDS
                     QEKGQAFQDIQGDVYEFLLSEIATAGKNGQFRTPRHIIKLMADLVQPQLGQRIADPAC
                     GTGGFLLGAYQYILTQLSLSQNLKRDNSKGSTHDEDGFFRTSVTAALTKKARILLQES
                     LYGYDIDATMVRLGLMNLMMHGIDEPHIDYQDTLSKSYSEETKYDIVLANPPFTGSID
                     RGDINENLKLSTTKTELLFVENIYRLLKKGGTACVIVPQGVLFGSGKAFKELRQTLVE
                     RCDLKAVITLPSGVFKPYAGVSTAILLFSKVFGPGDKISKPATDYVWFYEMSSDGYSL
                     DDKRNKLEGYGDLQDIIQKYHSRDEASDTDRTAKCFMVPRIDIEAENYDLSLSRYKEE
                     IFEEVQYEQPEAILERLIQAEVGNVDKKVLNKVQSGILYELLELKGMIV"
     gene            9438..11003
                     /locus_tag="L2X49_002508"
     CDS             9438..11003
                     /locus_tag="L2X49_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBN4797581.1"
                     /translation="MNRMTIPLGDILTQSGHHRAGNRELPVLSITMKNGLVDQSDKFK
                     KRIASSDTSKYRIVYKNELVVGFPIDEGVLGFQTKYPVGIVSPAYGIWKLKDESVCHI
                     PYLERYLRSSEARRLYASRMQGVVARRRSLTKSDFLSLEVPFPPINDQARIANLLAKV
                     EGLIEQRKQLLQYLDDLLKSVFVDMFSDPVKNAKGWELTTIGELAVDVRYGTSVSAQG
                     GKYKYIRMNNITPDGYWDFENLKYIDVDNKDLDKYSLQKGDLVFNRTNSKELVGKTAV
                     YDRDETVIIAGYLIRVRFDQQTNPWFVWGHLNSKFGKAKLFNLCRNIIGMANINAQEL
                     RAIPILKPPLELQNKFATIVEKAHAIKFRYQQSLADLETLYDVVSQKAFKGELELSRV
                     PIPTQIFFPVSGEEPEHGDAVQHIAVGNYLPVTNSLPSALDNAEARKSLIEEWLEAYI
                     AQLGNSSFSVHEFMMTAQARLAELYPDVDSKFGESDYEHIKAWVFKALAIGTLKQVFN
                     DADNCIELKAVLV"
     gene            11000..12784
                     /locus_tag="L2X49_002509"
     CDS             11000..12784
                     /locus_tag="L2X49_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction system-associated AAA family ATPase"
                     /protein_id="HBN4797582.1"
                     /translation="MKLLRLKISDPSGFRSLPCGFEHYFRTEWDLQEELNQHEGFAPF
                     VCAGPNGSGKSNLLEALAAIFFQLEILRVRRSFLPEVLQSTDHDLSPISFELDYLIRV
                     PEEFRISGGQEWAKVSVWKNNGESVRFHWVNQSDFDTNADEVFKGSHADILLPQYVLG
                     YSSGENEILSLPFFKMRFVQFDEYWNALTRQLSYSGHPESRLAYLDSGFSQAILLCNL
                     LFQNETALQPFREDVGIEALREFRIIIRRSIPLAPEQLTSFASEDKNQHQSLDDILNS
                     NPALHVDMDEESGQSYHLNLMQLLEGDDKSSLVVSALKRCASLYYEDECNDTLILDYW
                     VNDATRQAFRENFNGSALALFQAFQVLLTLNLYKVSDNLKTDLYRSTSHYVSETVPTL
                     ASDERIMRFKFVRFTKQGVEEPMMLKELSDGEHQLLHSLGLCLLFRETNSLFLLDEPE
                     THFNPHWRASFITRMRQCLCNTENVEQEMLITTHTPFLISDSKPEKVLVFSKDKYSGA
                     VTISIPKYNTLGASINKITMNTFGKRETIGGHAQAVLDDLRRRFNEGIEDKETLITEI
                     DEQLGDSVEKVLLLKAIFDSDNPTNDEV"
     gene            12790..13848
                     /locus_tag="L2X49_002510"
     CDS             12790..13848
                     /locus_tag="L2X49_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797583.1"
                     /translation="MLFSYTYVPHKMEKMQAFIDFIFYEVWCKARVRGPFCLKLFEAN
                     AELYEVMEDFSSSDTQGAVFFYNHVEKIYGLFSSLTEVQIDQFKQWYQGNNDLEKICA
                     NDPSIQVVRYSDIAIHHKDIAEQLAVFFKGLYSQSLLDLAVLRAKIGDIHDHYQSFAA
                     VNKAGKCPFCGIGDIKGGNHSKREAYDHYLPKALYPFNSINFHNLAPACHECNSTYKL
                     SKDPIQSGALRRKAFNPFASVDHVIQLQITLQHANIDALEPADIIIQFGPDTLEEELE
                     TWKDLYGIEERYKAKVCAENDGKYWLTQVLDEWKEEGLSPTEFMRTLARQAKKKPYAE
                     CNFLKEPFLKACHRIGVF"
     gene            13990..14351
                     /locus_tag="L2X49_002511"
                     /pseudo
     CDS             13990..14351
                     /locus_tag="L2X49_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611473.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            14915..15862
                     /locus_tag="L2X49_002512"
     CDS             14915..15862
                     /locus_tag="L2X49_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HBN4797584.1"
                     /translation="MTISLYTSRTSAPNTAAATSVSSLEQSGSSKTKFSKTRTDIYQT
                     VTDSIIAALEAGVKPWSCPWLRVSGMSGLPSNYATGTAYSGMNIMLLWCSASKQGFSD
                     SRWMTYKQAQAAGGQVRKGEHGTTAIFYTTLEKENDAGEVEQIPMLKTFTVFNVQQID
                     GLPLTTETVSPEATFNPLPQAENLLRKSGANIIEKGQNAFFSPSTDEVWLPERHLFSD
                     AANFYATGLHELVHWSGGKKRLNREMKGKFGSADYAEEELVAELGSAFLMADLGIEGE
                     VQHESYIASWLKALKNDKRYIFKAVSAASKAHSYLMDKL"
     gene            complement(16056..16802)
                     /locus_tag="L2X49_002513"
     CDS             complement(16056..16802)
                     /locus_tag="L2X49_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HBN4797585.1"
                     /translation="MLISSFHERQTRNNEKIKQLLNFLKEETYSDFKTLMLLFGFRDH
                     KSLYSLLAKTERMGLIQKHVLESRTMKISLWGITSDGLAVVLTPDDKIFPPRFEPSKI
                     TGWTLEHHLDNQAARLILEKKGASGWINGDRATFLSQYQVKHRPDGLIILPGSTVIAI
                     ETERRLKTRARYQSIIASHLLARTRKLWIHVFYVIPDPQKKRALELLFDSIRHVIINH
                     QHVPLEERHRRVFRIYTLDELKRLSLDSYT"
     gene            complement(16812..18671)
                     /locus_tag="L2X49_002514"
     CDS             complement(16812..18671)
                     /locus_tag="L2X49_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HBN4797586.1"
                     /translation="MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFAS
                     MKSDERQKAPLWKKLIIAFSLLCFLSGTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRI
                     AFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLERELRTDVRTVKSLLPETLHYNPL
                     DYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILLYQSILAGE
                     GVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAG
                     FSLAEKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGK
                     IEELALLNAINSPEGFSLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRD
                     RVIDVPRPIAVYLSELKYHLSRSALEGLGAARDKGVHIIMDHQSIADLKDCPADLKGD
                     AVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDELRKAKTDNMLTETIDSERT
                     IRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVSPDIAAT
                     VAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF"
     gene            complement(19381..19686)
                     /locus_tag="L2X49_002515"
     CDS             complement(19381..19686)
                     /locus_tag="L2X49_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dpoa decarboxylase"
                     /protein_id="HBN4797587.1"
                     /translation="MINEDLFIKNIHSKNQDRISVAMVYDTLSKEAHRGCGLYYEIYE
                     SCFIGLLRDHLSELNEADANRLRRYAESKGTKIDDASYSEALEAERECRAEIYREQM"
     gene            complement(19720..20025)
                     /locus_tag="L2X49_002516"
     CDS             complement(19720..20025)
                     /locus_tag="L2X49_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBN4797588.1"
                     /translation="MKKTVIAATVLCLTAGTPIYSFAADPCEVVLCMYGKATGNSGGS
                     ECSSAERAFFKINAFKKHHRFNPGKTFDMRKEFLGECKAADPESISKILSKFGRVKG"
     gene            complement(20061..20459)
                     /locus_tag="L2X49_002517"
     CDS             complement(20061..20459)
                     /locus_tag="L2X49_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="HBN4797589.1"
                     /translation="MIKKLKVIMLAAIVTGCSSPPPPVPVEWDKAAAPLNTSLSQWSD
                     NNVIVPSPTVNGKWTSSISAQSFYDTIWTPAVFYAVAHSTRIVVTAQSGTDFFNAKNW
                     LRQNGAKGVIEYQPVFNCLTCRETTIYFSR"
     gene            complement(20456..21481)
                     /gene="virB11"
                     /locus_tag="L2X49_002518"
     CDS             complement(20456..21481)
                     /gene="virB11"
                     /locus_tag="L2X49_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HBN4797590.1"
                     /translation="MTAKNISLDRYKQRFFGDFLELPGLTEIAVNRPGELYTKINGVW
                     EQHAVPLSYEDCYNFARCLAKHHGDNIEDIKPGLSATLESGERCQVIVPPACERDTVS
                     ITIRKPSKVQIPHQSYIDAGFYNRVTGEEKTETHDEELIALYNTKNIPLFMEKCVEYG
                     KTLAVAGETSTGKTTYMKMLIGYIPVHLRISTIEDNPEITFFIHKNYVHLFYPSESSD
                     EKGSIVTPASLLRWNYRMNPDRILLTEVRGAEAWDFLKITGSGHEGSMTSIHAGSAKE
                     AIYGFITRCYENPQCAQLPYTFMLRKVLDSLDVIVSIDLDGNVRRMNDIYFRPIHRNQ
                     YFEEMKA"
     gene            complement(21471..22721)
                     /locus_tag="L2X49_002519"
     CDS             complement(21471..22721)
                     /locus_tag="L2X49_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HBN4797591.1"
                     /translation="MNDENKMPVPDEAQAFPAHAEDDIATLEREARAKREAELLNAQD
                     DEEKDPVQPAVNKLKKRKRGKATAFLAIVAVTLIFLAWGGNWVYRNILWQPTEEKKQD
                     TAPQTNKTDYRQRNDLGMSTDTAEEEPEQQDNGQSSTGGTGQVAPAAPPELNKASFLI
                     RRDGSATTQNPVKTRQQEMTLTSATTTGQQDSSAATNTAAPPPGQSPAPLRRIPYNPD
                     LYIPENTSIPCSLDRRFVSDRAGKLRCTVTTDIWSASGNTKLIEKGTTASLLYRAIAE
                     EGMKHGQGRAFIIATKLRTRQPPYLDIPLVDTSAAGELGEAGVDGWIDSHFWERFGGA
                     LMVGMIPDIGAWASNSAGKKDRNTDYTENSRQAMADMARTTLENSINIPPTLYKNQGE
                     IINLITGEDIDFSNIYTLRLKNDR"
     gene            complement(22757..23665)
                     /gene="virB9"
                     /locus_tag="L2X49_002520"
     CDS             complement(22757..23665)
                     /gene="virB9"
                     /locus_tag="L2X49_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HBN4797592.1"
                     /translation="MMKKLTFTALALMMCGAAWAAAIPQASRYDSRVQQVIYNPQNVT
                     VVNTKPGFMTTLVFDNDEAVISAKPGFDEAWEATPDANRVNVRPVALTQGAPGEDGNT
                     TQVVIPPNSRDWHTNMLVVTSKRLYNVELNVIDDKSAQQPAFQVSYRYPGEERDKASR
                     EATARQREWEQKQQQASVQKALNSAQTPRNWSYTKYPGKGSFNIVPDFAYDDGRFTFV
                     GFSPSKSIPSVTKELNGKEHVVNSSIQKKGDFTVLVIQEVTPRLVLRSGNAVVGLENS
                     GFGKVHAADGSTVSRQVERVEKPESN"
     gene            complement(23662..24345)
                     /locus_tag="L2X49_002521"
     CDS             complement(23662..24345)
                     /locus_tag="L2X49_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBN4797593.1"
                     /translation="MSEQKLIAESRTFEQQMIERDKRATKAGFVVGGMGLLIAVLALV
                     AVVVMLPLKQTDVELYTLDNHTGRVEHVTRTSKTSLTATEAYQKAMAANYVKVRERYV
                     YPSLQDDYETVQVYNAPQVNDDYLALYAGKNAPDKVYKNGAHTVKVEILSNQITDATA
                     PDRVATIRYKKIIRRLADNSTRNEYWDARFTFHSNPDKEMSDAEREINYFGFTVTSWQ
                     TDREIRGGE"
BASE COUNT         6184 a         5504 c         6119 g         6632 t
ORIGIN      
        1 gaccgtcatc cccggttcaa agctctgctg aacaattgcg atcttttcct gtgtggtacg
       61 ccgtctgcgt ttctccggcc ctaagacatc aatcatctgt tctccaatga ctagtctaaa
      121 aactagtatt aagactatca cttatttaag tgatattggt tgtctggaga ttcagggggc
      181 cagtctaatg acgagtggta ctccgcatgg ttatttaact tctcagcacc actaatgatc
      241 ctgagcaact tcgtatttgg gccattgcca tggagtagaa agctgtgtct gagaatgcag
      301 aatttgagaa aagaattagc tttcttggag agcttctgaa aatggcacgc taacaacgtt
      361 ttgttaaaaa atgtgaatca cttgccgggg ctgcattttc tattcgagga gaatgttgac
      421 gtagatatta cagaaatctc agcgcatcag gataaactgc taccttaggt caaccaccaa
      481 aagaaaatac aatacgtcca gtttgtatgc ctttaataac acatcttcta aatgttgaag
      541 aattatcccg cctgctgaag aacgttgctc tgagtgtgat aaaccttttt attcttgatg
      601 atgtcagcga gaagatggaa tatatccccg actgggtcgt ggttacccgg ttaaacatct
      661 ggtggcgcta ataaatctgg caggtcacat tctgttgaat gctgtgacag tgccttaccg
      721 agagagcgaa attgtggtgt tctagtgcat agtcgccacc attccggata attctattgc
      781 gttgttttac aagttctttt acagcgcaga tctgtcgctg acgttgaatc aatagcctgg
      841 atttcagaac acaatgcctg tttgctggtt ggtccggcag tagcattaat tcattatctg
      901 aatgaaatgt gtcaatccac agaaaccgct gtcaatgacg cattttgtcg gacgttaaca
      961 cgagaaagtt tggctattcc gtaatgccat cttatttcgt aatggattga gaatgctgga
     1021 tttttaatta tattacgcgc agtattaatc gcgtaaatgt tttgcggtca gcaacggtgg
     1081 gggatccatt gttctgttca atgaatacca tcatacattt gaatcaggag tcggtatgat
     1141 gattttggtg acggggggcg cacggagcgg gaagagtcgc cacgcagagg tgcttattgg
     1201 ggactcttca caggttctgt atatcgctac ctcgcaaatc cttgatgatg agatggctgc
     1261 acggatagaa catcatcggc aaggccgccc ggagcactgg cgcacagtgg agcgctggca
     1321 acatcttgat gaattaattc atgcagacat taacccgaat gaggttgtgt tgctggaatg
     1381 cgttaccaca atggtgacta atctgttgtt tgattatggc ggcgataaag accctgatga
     1441 atgggattat caggcgatgg aacaggcgat taatgctgag attcagtcgt tgattgctgc
     1501 ctgccaacgt tgccccgcaa aggttgtatt agtgactaac gaagtgggaa tggggattgt
     1561 gccggagagt cgtctggcac gacattttcg tgatattgcc gggcgggtaa atcagcagtt
     1621 ggccgctgcg gcaaatgaag tatggctggt ggtttctggt attggagtaa aaatcaaatg
     1681 agtaaattat tttgggcgat gctctcattt attacgcgtc tgcctgtacc gcgtcgctgg
     1741 tcccagggac tggatttcga gcattattct cgcggtatta ttacttttcc tttgattgga
     1801 ttattgcttg gcgcgattag cgggctggtc ttcatggtgc tgcaggcatg gtgtggcgca
     1861 ccactggcgg cactgtttag cgtacttgtg ctggtgctga tgaccggggg attccatctg
     1921 gacggcctcg ctgatacctg tgatggcgta ttttctgcac gtagccgcga tcgcatgttg
     1981 gaaatcatgc gtgatagccg tttaggcacc cacggcggtc tggcattgat ttttgtggta
     2041 ctggcaaaga ttctggtgtt aagcgagttg gctctgcgtg gcgaatcgat ccttgcatcg
     2101 ctggcggcag catgtgcggt cagccgcggg actgccgcat tattgatgta tcgtcatcgt
     2161 tatgcgcggg aagaagggct tggcaatgtg ttcattggca aaattgatgg gcgacaaacc
     2221 tgcgtcacac tcggcttagc cgctattttt gctgccgtat tattgccggg tatgcatggt
     2281 gtggctgcta tggtggtgac gatggtggcg attttcatcc tcggtcagct tctcaaacgt
     2341 acgctgggcg ggcaaacggg cgatacgctg ggagcagcca tagagcttgg tgaactggtc
     2401 ttcctgctgg cactgctata aagagacgtg acatgcaaat acttgccgat ttactgaata
     2461 cgatcccagc catcgattct gccgctatgt cgcgtgcaca acggcatatt gacgggttac
     2521 tcaaacctgt tggtagcctg ggaaagctgg aggcgcttgc catacaactg gcaggaatgc
     2581 cggggttgaa tggcataccg catgtgggca aaaaagcggt actggttatg tgtgccgatc
     2641 acggcgtctg ggaggaaggg gtcgctattt ctccaaaaga agtgacagcc atacaggctg
     2701 aaaatatgac ccgtggaaca accggcgtgt gtgtgctggc agaacaagcg ggcgctaacg
     2761 tctacgtaat tgatgttggt attgatactg ctgagcctat ccccgggctt atcaacatgc
     2821 gtgtcgcacg aggtagcggc aatattgctt cagctccggc aatgagtcgc cgtcaggctg
     2881 aaaagttgct tttggacgtc atatgttata cgcaggagct ggcaaaaaac ggtgtcacgc
     2941 tgtttggtgt aggtgaactg gggatggcaa acacgacacc ggcagcagca atagtcagca
     3001 caatcactgg ccgggatcct gaagaagtgg ttgggattgg cgcaaacctg ccgacagata
     3061 aactggctaa taaaattgat gttgtgcgtc gggcgattac gttgaatcaa ccaaatcctc
     3121 aggatggtat tgatgtcctg gcaaaagtgg gtggatttga tttggtcgga atagctggag
     3181 tgatgttagg tgctgcttcc tgcggtttac ccgtgttgct ggatggattt ctttcttatg
     3241 ctgctgcgct cgcagcctgc cagatgtctc ctgcaatcaa accgtatctc attccttctc
     3301 acttgtcggc agaaaaaggc gcgcgtatag cgctctcgca tttggggctg gagccttatc
     3361 tcaatatgga tatgcgttta ggtgagggga gtggtgcagc tctggcgatg cccatcatcg
     3421 aagctgcttg tgcgatatac aacaacatgg gcgaacttgc tgccagtaat attgttctac
     3481 cggggaatac gacttctgat ttgaactgct aaaaacgaaa agtctgataa aagctatact
     3541 tatcggatag tattcgcgac ttaggaaagg gacatgatgc gtcgtgtaaa tattctttgc
     3601 tcatttgctc tgctttttgc cagccaaaat agcctggcgg taacttatcc attacctcca
     3661 gagggtagcc gtttagtggg gcagtcgctt actgtaactg ttcctgatca caatactcag
     3721 ccgctggaga cttttgccgc acaatacggg caagggttaa gtaatatgct ggaagcgaat
     3781 ccgggcgctg atgttttttt gccgaagcct ggctcacaac tcaccattcc gcagcaactg
     3841 attttgcccg ccactgttcg taaagggatt gtggttaacg tcgctgaaat gcgtctttat
     3901 tactatccgc cagacagtaa cacagtggaa gtctttccta ttggtatcgg ccaggctggg
     3961 cgagaaaccc cgcgtaactg ggtgactacc gttgaacgta aacaagaagc accaacctgg
     4021 acgccaacgc cgaacacccg gcgcgaatat gcgaaacgag gggagagttt gcccgcattt
     4081 gttcctgcgg ggcccgataa ccccatgggg ctgtacgcga tttatattgg caggttgtat
     4141 gccatccacg gtaccaatgc caattttggt attgggctcc gggtaagtca gggctgtatt
     4201 cgtctgcgca atgacgatat caaatatctg tttgataatg ttcctgtagg cactcgtgta
     4261 caaattattg atcagccagt gaaatacaca acggaaccgg atggttcaaa gtggctggaa
     4321 gttcatgagc cgctgtcgcg caatcgtgca gaatatgagt ctgaccgaaa agtgccattg
     4381 ccggtaaccc catctttgcg ggcgtttatc aacgggcaag aagttgatgt aaatcgcgca
     4441 aatgctgcgt tgcaacgtcg atcgggaatg cctgtgcaaa ttagttctgg ttcaagacag
     4501 atgttttaag agcgttggta attagaaagt aaaaagcctg ctcgaaagca ggcttttttt
     4561 gaatttggct cctctgactg gactcgaacc agtgacatac ggattaacag tccgccgttc
     4621 taccgactga actacagagg aatcgtgtga acggggcgca tattaatggc agtgcatggt
     4681 gatgtcaaag ggtgaaatgt atttttttgt ttgtttgctg ccaatttcaa caaagtggtg
     4741 agctttcaat cttattgtgt aatgaaccat cgtggtgcat accctccttt tatagggcag
     4801 gtgaatgcga ctgctgataa aaagggccaa tgacgaatct atcataatct tttatttgac
     4861 agttggttag cttgcgcatc aacgccaaaa tagaactggc aagcatcttg ctttctggtt
     4921 gtaagtaatg gcggcacttg ggccgattct taaaaaccgg aggcaacatg aacttcagac
     4981 gcctgaaata cttcgtaaaa attgtagata ttggtagcct gacccaggct gctgaagtat
     5041 tgcatatcgc acaaccagcg ctcagccagc aggttgccac actggaaggt gagttgaatc
     5101 aacagctttt gattcgcaca aaacggggcg ttacgccaac agacgccgga aaaattctct
     5161 atacccatgc acgggccatt ttacgtcagt gtgagcaggc ccaactggcg gtgcataacg
     5221 ttggtcagtc attatcgggg caagtctcga ttggctttgc gccaggaacc gctgcgtcat
     5281 ccatcaccat gcccttatta caggcggttc gcgctgaatt cccggaggtc gttatctatc
     5341 ttcatgaaaa tagtggtgca gtgcttaacg aaaaactgat aaatcaccaa ctcgatatgg
     5401 cggtgattta tgagcattcc cctgtggctg gtgtatccag tcgggcttta ctgaaagaag
     5461 atcttttcct ggtagggact caagattgtc cggggcaaag cgttgatgtt aatgctattg
     5521 cacaaatgaa cctctttctt cccagtgatt acagtgctgt tagacttcgt gttgatgagg
     5581 ctttttccct acggcgactc acggcaaaag tcattggtga aattgagtca attgccacgc
     5641 ttaccgcagc gattgccagc ggcatgggcg ttgcagtatt acccgaatcg gccgcgcgtt
     5701 cgttatgtgg cgcagtaaac gggtggatgt cacgcattac cacaccttcc atgagtctct
     5761 ctttgtcatt aaatttaccc gccagagcga acttatcacc acaggcacag gcagtgaaag
     5821 agttgttaat gtcagtgatg agttctccag tgatggaaaa aaggcagtgg caattggtga
     5881 gctaagcgtt atatcgggat ggaataagat gcgggttttt attatttgtt atgccgggca
     5941 ttagacttta acaatgacgg aaaatctgaa ctgcccggag tttaccgtga atttccaaca
     6001 actaaagata atccgcgagg ctgcacgtca ggattacaac ctgacagagg ttgcgaatat
     6061 gctttttacc tcacagtccg gcgtcagccg tcatattcgg gaactggagg atgaacttgg
     6121 catcgaaata tttgttcgac gaggtaagcg actgctgggc atgactgaac cgggcaaagc
     6181 attactggtc attgcagaac gtattctgaa tgaagccagt aatgttcgtc ggcttgcaga
     6241 cctgtttacc aacgatacgt ctggcgttct cactattgca acgacgcata ctcaggcacg
     6301 ttatagcttg ccagaggtca ttaaagcttt tcgcgaactt ttcccggagg ttcggctcga
     6361 gctaatccag gggacgccac aggaaattgc gacgttgttg caaaatggcg aagctgatat
     6421 tggtatcgcc agcgagcgtt tgagtaatga cccgcagctc gtcgccttcc cgtggtttcg
     6481 ttggcaccat agtttgcttg ttccactcga tcatccctta acgcaaatta caccattgac
     6541 gctggaatca atagcgaagt ggccgttaat cacttaccga cgggggatta cggggcgctc
     6601 acgtattgat gacgcatttg cccgcaaagg tttgctggca gatattgtat taagtgcgca
     6661 ggattctgat gtcattaaaa cctatgttgc tcttgggctt gggatcggat tagttgccga
     6721 acaatccagc ggcgcacaag aggaagagaa tttaatccgt ctggatacgc ggcatctttt
     6781 cgatgctaat actgtctggt tgggactgaa gcgaggacaa cttcagcgta actatgtctg
     6841 gcgctttctg gaactttgta atgcaggact gtcagttgag gatatcaagc ggcaggtgat
     6901 ggaaaacagt gaagaggaaa ttgattatca gatatagcaa aaagcccgct ataaaagcgg
     6961 gcttttcaga aatttggctc ctctgactgg gatcgcccaa gccaataact ggctaataaa
     7021 taagctaaac cttgttgctc ttcctgtcaa gaccaccata atgaccacca atttcgaatg
     7081 agtgtgcgac aaagcgtaga tgcgatgctt attactacag taaagtagaa caggctgagg
     7141 cgtcgttatg attggatggt aaccctgcgt atgaaacttg gatggctagc actttatccg
     7201 cctactgagt ggcgggatgc ttgcttgtta agcataggaa aaactctgca tttactgtct
     7261 tgaaaaaatc aatgtacata ggacgtgccc cacagacatc tcagggcaat gttgatgccg
     7321 tccgctctta cacaaatcgt tcagagttgg agtatgcatt tcatgtcgga tatcctgtgt
     7381 gaatggcgat gttttttggc actgaattta gtgaatattt tagcgggcgt gttggtagct
     7441 ctacgatttt tggataacct cttttcggga gctttttaga catttacact tttaaagcct
     7501 tctaatgata agtttttgtc tgttttatct ctttgtggta gaatataatg cttattattt
     7561 ggtggtttac tgagactcat cgccgatagg acattatgaa tactgttact aattgagcta
     7621 tttttactaa aaaacatata gttatgtcat ggtgcagcca aaatgggctg gtaagttgcc
     7681 actttaagaa aggaaaatgt tctgaaaaaa gagtaaactg ccagccacaa agcacctcaa
     7741 tagagactga accgagaacc cagccacatg ctacaaaaca accctgacct tcaaagcgcg
     7801 atcaaacgcc tctgggataa attctggagt agcggcatta gcaatccgct cacagccatt
     7861 gaacagatta cctacctgtt gtttatgaaa cggctagatg aactcgatca taaaaggcaa
     7921 gacgaaggtg aaacgagtgg tgagaaatac atttctaagt ttgctggtac ttggattcca
     7981 ccagaggaaa gaaaccgccc tgttgcggaa caacacccta tagataaacg cacgcttcgc
     8041 tggagcgagt tcaagaaatt gcagcccgaa gagatgctgc aacatgtgcg agacaaagtt
     8101 tttccttttc tgaaagatct gaatggtgct gaatctaact tcactcatca catgaaaaac
     8161 gcagtattca ttatcccaaa gtcagcattg ttggtggagg cagtgaaggc cattgacgaa
     8221 atcttcgaga tgatgaaaag ggattctcaa gaaaaaggcc aagcttttca agacattcaa
     8281 ggagatgtat atgaattcct gctgtcggaa atcgccaccg caggcaagaa cggccaattt
     8341 cgcacaccac gccacattat taaattgatg gcagatctgg tccagccgca actgggacaa
     8401 cgtattgccg accctgcctg cggtacgggg gggtttctgt taggtgccta ccagtacatc
     8461 cttactcagt tgtctttatc tcaaaactta aaaagagata attcaaaagg atctactcat
     8521 gatgaagatg ggttctttcg cacttctgtg actgccgctc tgaccaaaaa ggcccggatt
     8581 cttttgcaag agagtctgta tggttatgac attgacgcta ctatggtgcg tttaggccta
     8641 atgaacttaa tgatgcatgg aattgatgag ccgcatattg attatcagga cacactaagc
     8701 aagagctata gtgaagaaac caaatatgac atagtgctag caaacccgcc ttttactggc
     8761 agtatagaca ggggggatat taatgagaat ttgaaactct caaccactaa aaccgaactt
     8821 ttgtttgttg agaacatcta tcgtttgctg aaaaagggcg gtaccgcctg tgtgattgtg
     8881 cctcaaggag tattgtttgg ctcgggcaag gcattcaaag aactacggca aactttagtt
     8941 gagcgctgcg acctcaaggc tgtcattact ctgcccagtg gagtgtttaa accctacgct
     9001 ggagtaagta ctgccatttt attgttctcc aaagttttcg ggccggggga taaaatttcc
     9061 aaacctgcta ccgattatgt ctggttttat gagatgtctt ccgatggtta ctcattggat
     9121 gacaaacgaa ataagcttga aggctacggc gacctgcaag acatcattca aaaataccat
     9181 tcccgtgatg aggcgtctga cacagatcgt acagctaagt gttttatggt gccaagaata
     9241 gatattgaag cggagaatta cgatctttcg ctgtctcgct ataaggaaga aatatttgaa
     9301 gaagtgcagt acgaacagcc tgaagctatt ctggaacgac taattcaagc agaagtgggg
     9361 aatgtggata agaaagtgct taataaagta caaagtggca tcttgtacga gctactagag
     9421 ttaaagggga tgatagtatg aatagaatga ctattcccct tggtgacata ttaactcaat
     9481 ccggtcacca ccgtgcaggc aatcgagagc ttccggtcct atcaataacc atgaaaaatg
     9541 gtttggttga ccaatcagac aaattcaaga agcggattgc cagttcagac acgtctaaat
     9601 atcgtattgt atacaagaat gaacttgttg ttgggtttcc tattgacgag ggagttcttg
     9661 gattccagac aaaatatccg gtaggaatcg ttagcccagc ttatggtatc tggaaactca
     9721 aggatgaatc ggtatgccat atcccttacc ttgagcgtta tctgcgctcc tccgaggcaa
     9781 ggcgactcta tgcatctcgt atgcaagggg ttgttgcacg tagacgtagc ttgacgaagt
     9841 ctgattttct tagtttggaa gtaccttttc ctcccatcaa tgatcaggct cgtatagcta
     9901 atttattggc caaggtagaa gggctaattg aacagcgtaa acaacttttg caatacctgg
     9961 atgacctact aaagagcgta ttcgtcgata tgtttagtga tccggtaaaa aatgcaaaag
    10021 gatgggaact aacaacaata ggtgagttag ctgtcgatgt aagatacggg acaagtgtat
    10081 cagcgcaagg tggaaaatat aaatacatac ggatgaataa cattactccg gatggttact
    10141 gggattttga gaatcttaaa tatatcgatg tagataataa ggatctggat aaatattctc
    10201 ttcagaaagg agatctcgtt ttcaaccgga ccaattcaaa agaattggtt ggtaaaacgg
    10261 cggtttacga tcgagatgaa actgttatta ttgctggcta tctcattcgt gttaggttcg
    10321 atcaacaaac taacccttgg tttgtatggg ggcacctgaa ctcaaagttt ggaaaagcaa
    10381 aattatttaa tctttgtcgt aatattattg gtatggctaa cattaatgcc caagagttaa
    10441 gggccatccc tattcttaag cctccactgg agttacaaaa taaattcgct actattgtag
    10501 agaaagcgca tgctattaaa tttcgctatc agcaaagtct tgctgatctt gagaccctgt
    10561 atgatgttgt tagccaaaag gctttcaagg gggaattaga actttcgcgt gtgcctattc
    10621 cgacacagat atttttcccg gtatcaggtg aagaacctga acacggggat gccgtacaac
    10681 atatagcagt gggtaattat ctgcccgtaa ctaatagctt accatccgcg ttggataatg
    10741 cagaggctcg caagtcactg atcgaggaat ggctagaggc ctatatagcg caattgggca
    10801 actcatcgtt ttcagtacat gagttcatga tgacggcgca ggcacggctg gcggaattat
    10861 atccggacgt tgattctaag tttggtgaaa gtgactatga gcatatcaaa gcttgggtgt
    10921 tcaaagctct ggctattggt acactgaagc aggtattcaa tgacgctgac aattgcattg
    10981 aacttaaggc ggttttggta tgaagctatt acgcctaaaa atctctgatc cgtccggctt
    11041 ccgtagtctg ccctgtggtt ttgaacacta ttttagaacc gaatgggatt tacaggaaga
    11101 attgaatcaa catgaaggct ttgcgccttt tgtgtgtgct ggtcccaacg gcagtggtaa
    11161 atccaatctg cttgaagcat tagcagctat cttctttcag ttggaaattt tgcgggtccg
    11221 tcgaagtttt ttgcctgagg tattgcaaag taccgatcat gatttatcac caatctcatt
    11281 tgagctggat tatctaattc gagtaccaga agagtttcga atctctggcg gacaagaatg
    11341 ggcaaaagtc tccgtgtgga aaaacaacgg ggaatctgtg cgtttccatt gggtaaatca
    11401 gagtgacttt gacacaaatg ctgatgaagt tttcaaggga agccatgccg atattctatt
    11461 gcctcagtat gtgttgggct actcgtctgg cgagaatgaa attctcagtc taccgttttt
    11521 caaaatgcgc tttgtacagt tcgacgagta ctggaatgcc ttgactcgac aactgagcta
    11581 ctctggccat ccggagtcga ggttggccta tcttgacagt ggtttcagcc aagctatttt
    11641 actttgtaat ctgctttttc aaaacgaaac tgcgcttcaa cctttccgtg aggatgttgg
    11701 tatagaggcg ttgcgggagt ttcgtattat tatccgtcgc agtattcctc ttgcgccgga
    11761 gcagttgacc tcgtttgcca gcgaggataa aaaccagcac caatcacttg atgacatttt
    11821 gaatagtaac cctgctctgc atgtggatat ggatgaagaa agtggtcaaa gttaccattt
    11881 aaacctgatg cagctgctgg aaggggatga caaatcctcc ttggtagtca gcgcgcttaa
    11941 acgctgcgct agcttgtatt acgaggacga gtgtaacgac actctaattc tggactactg
    12001 ggttaatgat gctactcgac aggcttttcg cgagaatttt aatggttcgg cactggctct
    12061 attccaggcg ttccaagtat tgctgacact caatttgtat aaggtgagtg ataatctcaa
    12121 aactgatctg tatcgctcaa caagtcacta tgtcagtgaa acggtaccaa cgttggcgtc
    12181 cgacgaacga attatgcgct tcaagtttgt gcgttttact aaacaaggag tggaagagcc
    12241 aatgatgctc aaggaactct cggacggtga acaccaattg cttcatagcc taggtctatg
    12301 tttactgttt agagaaacca acagcctgtt tctgttagac gaaccggaga ctcatttcaa
    12361 cccacattgg cgtgccagtt ttatcactcg gatgcgccaa tgtttatgta acactgagaa
    12421 tgtggagcag gaaatgttga tcactactca tacacctttc cttatctcag atagtaaacc
    12481 cgaaaaagta ctggtttttt ctaaggataa atatagcggt gcggtcacaa taagcatacc
    12541 taaatacaac acacttggtg catcaatcaa taaaattact atgaatacct tcggcaaacg
    12601 tgaaactatc ggtggccatg cgcaagcagt actggatgat ttacgcagac gttttaacga
    12661 aggcattgaa gacaaggaaa ctctgatcac agaaattgat gaacaattgg gtgactcggt
    12721 ggaaaaagta ttactcctca aggctatttt tgatagcgac aatccaacga atgacgaggt
    12781 ttagcactga tgctgttttc atatacctat gtgccacaca aaatggaaaa gatgcaggca
    12841 ttcattgatt ttatttttta tgaggtatgg tgcaaagcgc gggtaagagg gcctttttgt
    12901 cttaaactgt ttgaagctaa cgcagaattg tatgaagtga tggaggattt ttcttcctcg
    12961 gatacacaag gtgctgtttt cttttataac catgtagaga aaatttatgg cttgttttcg
    13021 tcactgactg aggtacaaat cgatcagttt aaacagtggt atcaagggaa taatgatctg
    13081 gaaaaaatat gtgctaatga cccttcaata caagttgtcc gttattccga tattgccatt
    13141 caccacaaag atatagcaga acaacttgct gttttcttta agggcttata ctcacagtca
    13201 ctgcttgatc tcgctgtact gcgtgccaag atcggtgata tacacgatca ctatcaatct
    13261 tttgcagcag tgaacaaggc gggtaaatgt cctttttgtg gtattggaga tataaagggt
    13321 gggaaccata gcaagcgcga ggcctatgac cattatttgc ccaaagcttt atatccattt
    13381 aattcgataa atttccataa tcttgctccg gcctgtcatg agtgcaatag cacatataag
    13441 ttatccaaag atcctattca gagcggcgct cttcgtcgta aggccttcaa tccatttgcc
    13501 tcagtggatc atgtaattca attacagatt actctacaac atgcaaatat cgatgcgctt
    13561 gagcctgctg atattattat acagttcggt cccgatacgc tggaggagga actagaaaca
    13621 tggaaagact tgtacggtat agaagaacgt tataaagcca aagtttgtgc agaaaatgac
    13681 ggtaaatact ggcttactca ggtgctagat gagtggaagg aggaggggtt gagtcctacc
    13741 gaattcatga gaacattagc acgtcaagcg aaaaagaagc cttatgctga atgcaatttc
    13801 cttaaagaac cttttcttaa agcttgccat cgaataggtg ttttttgagg ctcaatacat
    13861 atcaagctat caatttgttt ataacttact gttataaaaa aacatcaaac ggtggctttg
    13921 ccctgctccc gattgattaa catacaccga tgttagtaac gtcttcatta gtcacatgag
    13981 gacatcataa tgaagaagcg tttttccgac gaacagatca tcagtatcct ccgcgaagct
    14041 gaatctggag tttttgcccg ggagctttgc cgcaagcacg ctatctctga tgctgcgtac
    14101 tacacttggc gcaagaagtt tgatggcatg gaaatgcctg aggtgaaacg tcttaagtca
    14161 tttgaagaag agaacgccca cctcaaggag ttgcttgcag aagtcatgat ggataaagaa
    14221 gcgttgcagg tggccctgaa ccgaaagttc tgacgacaga ccagaagcgg gaagctgtgg
    14281 aagtcatgtg tgaggcaacg ggtctgtcgc aacgtcacgc cagtaggctg gcaggtctct
    14341 ccctgtcaac ataatacagg gggaatgtca gtttgcatgg gcgcatactg atagaggggc
    14401 actgagaata agacagatcg ccactacggc atatgatgcc cgcggttacg gcgctgtggc
    14461 ctgtttcact aaatcgctca ctatgggtgg tagtgaataa actgtacgcg gcgctcaata
    14521 aggggacggc agggactcgg tagtgattga tgccggaacg aggcatttta agaacaaaac
    14581 gcccctggtt agcagtatca cctcgtccgg caggaaagca tgctaacatc aacaagaacc
    14641 tcctgttaat gccatttccc gactcattac caccaatcta atttcaccgc tgcgaagtaa
    14701 ggggcgttaa gcccggctat gcaggggcag gttttctctc ccgtgcgctt ttccgttctt
    14761 catatctgct tccggttaat cgtttcccaa cacaacccgt cccgcaaggg taaaattgca
    14821 cgcatactgc gcccttgcag gacagaacga tgccgggaaa gcgaaccgtc aggcgatacg
    14881 aagacgaaaa tcacacactg acggagaaaa aaacatgacg atttcccttt atacctcccg
    14941 gacaagcgcc cctaacaccg cagcggcgac cagcgtatcc tcgctggagc agtcaggctc
    15001 ctcaaaaacg aaattttcta aaaccagaac ggatatttat cagaccgtta ccgacagcat
    15061 cattgcagcg ctggaagccg gtgtaaaacc gtggtcctgc ccgtggctgc gtgtatccgg
    15121 catgtctgga ctgccatcca attacgccac tggcacggct tacagcggaa tgaatatcat
    15181 gttgctgtgg tgcagcgcat ctaaacaggg tttcagcgat tcccgctgga tgacttacaa
    15241 acaggcacag gcagcaggtg gacaggttcg caagggtgaa catggcacaa cagccatttt
    15301 ctatacgacc ctggaaaagg aaaacgacgc cggagaggtt gaacaaattc cgatgctgaa
    15361 aactttcacc gtgtttaacg ttcagcaaat cgacggtttg cccctgacaa ctgaaacagt
    15421 cagcccggaa gctacattta acccgttgcc gcaggctgag aacctgttac ggaagagtgg
    15481 cgcaaacatc atcgagaaag gacaaaacgc ctttttcagc ccatcaactg atgaagtctg
    15541 gttaccggag cgccatttgt tttcggatgc ggctaacttc tacgctaccg gtctgcatga
    15601 gctggttcac tggagcgggg gtaaaaaacg cctgaaccgt gaaatgaaag ggaagtttgg
    15661 cagtgcggat tatgcagagg aagaacttgt cgcggagctg ggaagtgcct ttctgatggc
    15721 tgatcttggc attgagggag aggtacaaca tgaaagctat attgcttcct ggctgaaagc
    15781 gctgaaaaac gacaagcgtt atattttcaa agcggtcagc gccgcctcta aagcgcattc
    15841 ttatctgatg gataaactct gaggacggag ccagacaacc gcaagggaat gcggttacat
    15901 ggtttttaac cggcaacgct gcagggcgca gactgaggcg gaacgcagca tagcgagtac
    15961 cgaacagtct gcgcctcgca gtggaataaa aatataccgg gctggcggta ttaccgccag
    16021 cccggtgaga ccatcacgat ggcctgagta aaagcttacg tatagctgtc taatgacaag
    16081 cgctttaatt catcaagggt ataaatgcga aatacccgac ggtggcgttc ttccagcggg
    16141 acgtgctgat ggttgatgat gacatgtctg atgctgtcaa ataggagctc aagtgcccgt
    16201 tttttctgtg ggtcaggaat gacataaaaa acatgaatcc acagtttgcg ggtacgggcc
    16261 agcaggtggc tggcaataat cgactggtag cgggctcggg ttttcaggcg acgctcggtt
    16321 tcaatggcga tcacggtgct gccaggcaag atgatcaacc cgtccgggcg atgttttacc
    16381 tgatactggc tgagaaaggt ggcccggtcg ccgttaatcc atcccgacgc gcctttcttc
    16441 tccagaataa gcctggcggc ctgattatca agatggtgtt ccagcgtcca gccggtgatt
    16501 tttgatggtt caaaccgagg aggaaagatt ttatcatccg gggttaacac caccgccagc
    16561 ccgtcgctgg tgatccccca taatgaaatc ttcattgttc gtgattcaag cacatgcttc
    16621 tggattaacc ccattcgctc tgtttttgcc agtagcgaat aaagcgactt atgatcccgg
    16681 aaaccaaata acagcatcag agttttaaaa tcactgtaag tttcttcttt caggaagttc
    16741 agcagttgtt ttattttttc gttgttacgc gtctggcgct cgtgaaatga tgaaatcagc
    16801 ataagcgctc cttaaaaatc cagcggggac aggtgttctt catcatcagg tttcggcttt
    16861 accggtttga cttcgctttc gtcgaagaaa agagccgggt gtgttgtcat cacatccgac
    16921 atggcgggtt tatcatcctc gcaaaaatcc agcatgatct tcacgggggc taccgttgcg
    16981 gcaatatcag gggaaaccga aaaagttttt agctggcgct ttctgacctt tatcggcgag
    17041 atcagtgaag cagaaggtag cgtttttgtc gtaaagataa agctgacaaa atcgggcagg
    17101 ttcaggatca tattgctgtc aataaagaaa cgttcggcct ggcggattgt gcgctcactg
    17161 tcgatggtct ccgtgagcat attatctgtt ttagccttac ggagttcgtc atccaccaga
    17221 atagtgcctg acattctcgc tacccattcc gctgtatccg ggtccataac ccggtaaacc
    17281 agtttgaatt tggcattttc aacgaccgca ccgacaacgg cgtcaccttt caaatctgcc
    17341 gggcagtctt ttaaatcggc aatggactgg tggtccataa taatatgcac gcctttatca
    17401 cgcgctgccc ctaatccctc cagtgctgac ctggataaat ggtatttcag ttcggaaaga
    17461 taaacagcaa tggggcgggg gacgtctatt actcgatcgc gcttttctgc cagctggtaa
    17521 aggcgaacca gtagcatgcg ctgggcggta ataattttgc tgtttcgcat ggagccgaca
    17581 acataacagc agccgccctc atcaaatatg tgcttaagcg aaaagccttc tggtgagtta
    17641 atggcattga gtaatgccag ttcctcaatt tttccgaaaa aggctttgat cttttcggca
    17701 atactctgaa cgtaatcacc gttataaaca tcccggattg ttgtcgatgg attatcgctg
    17761 acaaattgtg cggctatacg ggcggctttt cggtcatcaa tgcgataaaa atcagattcc
    17821 tgcccttttt ctgccaggct gaaacctgca acaaacaatt cttccagctc atcgggcgtg
    17881 atatcttcaa tcagatttaa ctggtattgc tgttttcgca ggtcaattaa ggcaaaaggc
    17941 ttgcctgcat cctcgcaggc tttgcggtaa agatgcggtg cccattcatc gtctttgggg
    18001 tccataacaa aaacaccttc accggccaga atactttgat aaagtaatat gccggcggct
    18061 acccctttac cggcccctgt ggtgccgata atatccgcgt gttgtttctg ccagtctttt
    18121 aacgggagat acattggttc attttccctg cccatgccgg taaaaatgcc tttattcagg
    18181 tcgatataat ccagcgggtt ataatgcagt gtttccggca gcagggactt tacggtgcgg
    18241 acatcggtgc gcaattcccg ttcaagggtg gtctttttaa caaggcgttt ctttattttg
    18301 tccagttccg gtgtgaatat ccggcgaagc ataatatgaa aaataatccc ggttacagtg
    18361 aaagcaatcc gtactgacca cgccagtacc gtattatcac tggtgggtct gatcttatac
    18421 agccactgaa aggatccgat aacgaccgga gccagtgtgc cggagagaaa acacagcagg
    18481 gaaaaagcaa taatcagctt tttccagagc ggcgctttct gccgttcatc gcttttcatt
    18541 gatgcaaaaa acggcatcgt cagccccgct aataatgcca gcaccagttg tttctgttgc
    18601 acaaaagcca gcaccgccag catcccgttc actatcgggg acagtgaagc ggtaagctta
    18661 tttaaaatca tcatgcctcc ttcgtcagcg attcgcccca tgacatcgcc ccatcaattc
    18721 gccccacggt gtcgggcgaa aagacggggc gagcgtatgg ggtaaagaag gtaaccgctg
    18781 tgcggccatg taccggaaag catgcttccc tgcacgaaac ggcgtgcgcc agtacatgcg
    18841 ggctggacgc ccttattgcc tgtcgcatgg gggtaatgtc gcggctggac gccacgccaa
    18901 aacccccagg gtcaaaggcg gcacaccgtc gcacgctacg cgcgaccaac ccctttaaga
    18961 cgcgcgttta gcctattttt tcttcgcaag ctcgaaaaaa tgggaacgct gctttaaagg
    19021 ggttggtcgc gcctaatcgg catattcccc gccggggcgg gaaatatagc ctggctgcga
    19081 cggtgtgcgg ggcaagcccc ccacaaaaac cggcacgtgg gcgtgccttt ttgctcgctg
    19141 gggcggcaaa atttttgcgg ctccccccgg cccgcttcaa ttcgctgggg cgaatgttcg
    19201 cgggtggacg tggatcctga aaattagcaa tttctccgaa attgaatttt ctgtccacgt
    19261 tccacttcat tatttttcgt cttaacaatt tcttcgaaat tgaagccaaa aataatgaag
    19321 ccttaaaatc ccgtcgcctg ttagtctctg tattgtacaa tacacttttg ttttgtgtga
    19381 ttacatttgc tcgcggtata tttcagccct gcactccctt tcagcttcca gtgcttcact
    19441 gtaggacgca tcatcaattt tcgtaccttt actctcggca tagcgtctta atctatttgc
    19501 gtcagcttca ttaagttctg aaagatgatc gcgcaaaaga ccgataaaac agctttcgta
    19561 tatttcgtaa tacagcccac aacctctgtg ggcttctttt gataacgtgt catataccat
    19621 ggctacagat attctgtcct gattttttga gtgtatattc ttaatgaata aatcttcgtt
    19681 tatcatttcg aacctctcat atcaaaatgg atatgtcagt tatcctttca ctctgccaaa
    19741 ttttgaaagg attttactta ttgattcagg gtctgcggct ttgcactccc ctaaaaattc
    19801 ttttcgcata tcgaatgttt tacccggatt gaaacgatgg tgttttttaa atgcgttgat
    19861 tttaaaaaat gcgcgttctg ctgaactgca ttcactccca ccactattac ctgtagcttt
    19921 tccgtacatg caaagaacaa cttcgcacgg gtcagcggca aaggaataaa tcggcgttcc
    19981 tgcggtcagg cataataccg tcgccgcgat aacggttttt ttcatgataa atccttatta
    20041 gattgtgttg cttaaatgga ttagcgagag aaataaatag tggtttctct gcacgtcagg
    20101 caattaaaga caggctggta ttcaataacg ccttttgcgc cattctggcg aagccagttt
    20161 ttggcattaa agaaatcagt gccggattgt gcagtgacga caattcgtgt tgaatgggcg
    20221 actgcgtaaa atactgctgg agtccatatt gtatcgtaga aactttgtgc gctgattgat
    20281 gatgtccatt taccgttaac tgtcggggaa gggactataa cgttattgtc actccattgc
    20341 gataaactgg tatttaatgg ggcagcggct ttatcccact ccacaggaac gggaggcggc
    20401 ggcgatgaac atccggtaac tattgctgcc aacatgatga ctttaagttt ctttatcatg
    20461 ctttcatttc ctcaaaatac tggtttcgat gaattggacg gaaatagatg tcattcatac
    20521 ggcgaacatt tccgtctaaa tcaatgctca caatcacgtc caggctatcc agaaccttac
    20581 gtaacataaa tgtatagggg agctgggcgc actgtggatt ttcatagcaa cgggtaataa
    20641 acccatatat agcttctttc gctgatcctg catgaatgga ggtcatactc ccttcatggc
    20701 cggaacctgt tattttaagg aagtcccagg cttccgcacc gcgaacctct gttaataaaa
    20761 tgcggtcagg gttcatacgg taattccagc gtaaaaggct cgccggggta acgatagaac
    20821 ctttttcatc tgatgattct gacggataaa aaagatggac gtagttttta tggataaaga
    20881 acgtgatttc cgggttgtcc tcaatggttg atatccgaag atgtacagga atatacccta
    20941 tcaacatttt catataggtg gttttcccgg tgcttgtttc cccggcgacc gcgagagttt
    21001 taccgtactc gacgcatttc tccataaaca gaggaatatt ttttgtattg tacaatgcaa
    21061 ttaattcctc gtcgtgcgtc tccgtttttt cctcgcctgt tacccggtta taaaatcccg
    21121 catcgatgta tgactgatgc gggatttgta ccttcgatgg cttacggatg gtgatagaca
    21181 ccgtatcgcg ctcacaggcc gggggaacaa tcacctggca ccgttcgcca gattccagcg
    21241 tggctgacag ccccggctta atatcttcga tattgtcgcc gtggtgcttt gccaggcacc
    21301 gggcaaagtt gtagcaatcc tcataggaca aaggaacggc gtgctgctcc catacgccgt
    21361 tgattttggt atacagctca cctggacgat tgacggcgat ctccgtcagg ccgggaagct
    21421 ctagaaagtc gccaaagaaa cgctgtttat agcggtcgag ggaaatattc ttagcggtca
    21481 tttttcaacc tcagtgtata gatgttggaa aaatcaatat cctcgcctgt gattaggttg
    21541 atgatctcgc cctgattctt atagagcgtt gggggaatat tgatgctgtt ctctagcgtg
    21601 gtacgtgcca tatctgccat agcctggcgg ctgttttccg tatagtccgt attgcggtct
    21661 tttttacccg cgctgttgga tgcccatgcg ccaatatcgg ggatcatgcc gaccattaac
    21721 gcgccgccga accgttccca gaagtgcgaa tctatccagc catcaacgcc agcctcgccc
    21781 agctcgccag ccgcgctggt atcaaccaac gggatatcga gataaggcgg ctggcgggtg
    21841 cgtagcttcg tcgcaatgat gaatgcccgc ccttgcccgt gcttcatacc ttcttccgct
    21901 atggcgcggt aaagtaatga tgcggtggtg cctttctcta tcagcttcgt gttgccgctg
    21961 gcgctccaga tgtcagtagt gaccgtgcag cgaagtttcc ccgcccggtc agatacaaag
    22021 cggcgatcca gtgagcaggg gatcgaggta ttttccggga tgtacaggtc agggttatag
    22081 ggtatgcggc gcagtggtgc ggggctttgt cccggcggcg gtgcggcggt atttgtggcc
    22141 gcgctgctgt cctgttgtcc ggtggtcgtt gccgatgtca gcgtcatttc ctgctgccgg
    22201 gtttttaccg ggttctgtgt ggtggccgaa ccatcacggc gaataaggaa gctggctttg
    22261 ttgagttctg gcggggcggc cggagccacc tgccctgttc cccccgtgct gctctggccg
    22321 ttatcctgtt gctctggctc ttcttcggct gtatcggtgg acataccgag atcgttacgc
    22381 tgccggtaat cggttttgtt cgtctgcggg gctgtgtcct gtttcttttc ttccgtaggc
    22441 tgccacagga tattgcgata aacccagtta ccaccccatg ccaggaagat aagcgtgacc
    22501 gcgacaatgg caagaaaggc ggttgctttg ccccgtttgc gctttttcag cttgttaacg
    22561 gcgggttgta ccgggtcttt ctcttcgtcg tcctgcgcat tgagaagctc ggcctccctt
    22621 ttagcgcggg cttcccgctc aagcgtggca atatcatcct cagcgtgtgc cgggaatgcc
    22681 tgcgcttcat ccggcacagg cattttgttt tcgtcgttca tatgcacgtt tccttttata
    22741 aaaatatttt tataggttaa ttggattccg gtttttccac tctttccacc tgccgggata
    22801 cggtggaacc atcggcggcg tgtaccttgc caaatccgct attttccaga cccacaacgg
    22861 cattaccgga acgcagtact aggcgtggcg tcacctcctg aatcaccagt acggtgaaat
    22921 cgcctttctt ctggatactg ctgttaacca catgctcttt gccgttcagc tctttggtga
    22981 cggaaggaat ggacttagac ggggagaagc ccacgaacgt aaagcggcca tcgtcgtaag
    23041 caaaatcggg aacaatgttg aaactgcctt tgcccggata tttggtgtaa ctccagttgc
    23101 ggggcgtctg cgccgagttc agcgctttct ggacgctggc ctgttgctgt ttctgctccc
    23161 actcgcgctg tctggccgtc gcctcccggc tcgctttgtc ccgttcttcg cccggatagc
    23221 gatagctcac ctggaaagcg ggttgttgtg ccgatttatc atcgatgacg ttcagctcga
    23281 cgttgtacag gcgcttactg gtgacaacca gcatattggt atgccagtcc cggctgttcg
    23341 gggggatcac gacctgcgtt gtgttgccat cttctccggg cgctccctgc gtgagcgcga
    23401 ccgggcgaac gttgacccgg tttgcatccg gcgtggcttc ccacgcctca tcaaagccag
    23461 gtttggcgct tatgaccgcc tcatcgttat caaagactag cgttgtcata aagccgggtt
    23521 tggtgttaac gacagtgacg ttctgcgggt tatagataac ctgctgcacg cgggagtcat
    23581 agcggcttgc ctgcggtatg gccgcagccc atgccgcgcc gcacatcatc agtgccagtg
    23641 cggtaaacgt taactttttc atcattcacc tccccggatt tcgcggtcgg tctgccaact
    23701 ggtgacggta aagccgaagt agttgatctc gcgttccgca tcgctcattt ccttgtcagg
    23761 gttagaatgg aacgtgaacc gggcatccca atattcgttg cgggtgctgt tatcagccag
    23821 acggcggata atcttcttgt agcggatggt ggcgacccta tcaggggctg tcgcatcggt
    23881 gatctggttg gacaagatct ccactttcac ggtgtgcgcc ccgttcttat aaaccttgtc
    23941 tggggcgttt tttccggcgt aaagcgccag atagtcatcg tttacctggg gagcgttgta
    24001 gacctgtacc gtctcgtagt cgtcctgcaa agagggatag acataccgct cacgcacttt
    24061 gacgtagttt gcggccatcg ccttttgata ggcttctgtt gcggtcaggc tggttttcga
    24121 tgtccgggta acgtgttcca cccgtcctgt gtggttatcc aaggtataca gctcaacgtc
    24181 cgtctgtttc agcggcaaca tcacgaccac ggcgacgagc gccagtaccg caatcagcag
    24241 acccataccg ccaacaacaa aaccggcttt tgtggcgcgt ttatcccgct caatcatttg
    24301 ctgctcaaaa gtgcgggatt cagctattaa tttctgttca ctcatgactg tttaactccg
    24361 ccataatttg aggggtgttt acgggttctc ccttaccgct aaaggcagag ggaggcgtgt
    24421 tctgggcgca gccagaaag
//
LOCUS       DAFNBJ010000067        24426 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000067 DAFNBJ010000000
VERSION     DAFNBJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.067"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            140..823
                     /locus_tag="L2X49_002522"
     CDS             140..823
                     /locus_tag="L2X49_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBN4797594.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            867..5717
                     /gene="ehaG"
                     /locus_tag="L2X49_002523"
     CDS             867..5717
                     /gene="ehaG"
                     /locus_tag="L2X49_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HBN4797595.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVCDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPSKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIRVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6085..7740
                     /gene="lldP"
                     /locus_tag="L2X49_002524"
     CDS             6085..7740
                     /gene="lldP"
                     /locus_tag="L2X49_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBN4797596.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7740..8516
                     /gene="lldR"
                     /locus_tag="L2X49_002525"
     CDS             7740..8516
                     /gene="lldR"
                     /locus_tag="L2X49_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBN4797597.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8513..9703
                     /gene="lldD"
                     /locus_tag="L2X49_002526"
     CDS             8513..9703
                     /gene="lldD"
                     /locus_tag="L2X49_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBN4797598.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9889..10362
                     /gene="trmL"
                     /locus_tag="L2X49_002527"
     CDS             9889..10362
                     /gene="trmL"
                     /locus_tag="L2X49_002527"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBN4797599.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10415..11236)
                     /gene="cysE"
                     /locus_tag="L2X49_002528"
     CDS             complement(10415..11236)
                     /gene="cysE"
                     /locus_tag="L2X49_002528"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBN4797600.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11316..12335)
                     /gene="gpsA"
                     /locus_tag="L2X49_002529"
     CDS             complement(11316..12335)
                     /gene="gpsA"
                     /locus_tag="L2X49_002529"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBN4797601.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12335..12802)
                     /gene="secB"
                     /locus_tag="L2X49_002530"
     CDS             complement(12335..12802)
                     /gene="secB"
                     /locus_tag="L2X49_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBN4797602.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12865..13116)
                     /gene="grxC"
                     /locus_tag="L2X49_002531"
     CDS             complement(12865..13116)
                     /gene="grxC"
                     /locus_tag="L2X49_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBN4797603.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13258..13689)
                     /locus_tag="L2X49_002532"
     CDS             complement(13258..13689)
                     /locus_tag="L2X49_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBN4797604.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13934..15478
                     /gene="gpmM"
                     /locus_tag="L2X49_002533"
                     /gene_synonym="pgmI"
     CDS             13934..15478
                     /gene="gpmM"
                     /locus_tag="L2X49_002533"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBN4797605.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15488..16771
                     /gene="envC"
                     /locus_tag="L2X49_002534"
     CDS             15488..16771
                     /gene="envC"
                     /locus_tag="L2X49_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBN4797606.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16775..17734
                     /locus_tag="L2X49_002535"
     CDS             16775..17734
                     /locus_tag="L2X49_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBN4797607.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17721..18755)
                     /gene="waaH"
                     /locus_tag="L2X49_002536"
     CDS             complement(17721..18755)
                     /gene="waaH"
                     /locus_tag="L2X49_002536"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HBN4797608.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18994..20019)
                     /gene="tdh"
                     /locus_tag="L2X49_002537"
     CDS             complement(18994..20019)
                     /gene="tdh"
                     /locus_tag="L2X49_002537"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBN4797609.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20029..21225)
                     /gene="kbl"
                     /locus_tag="L2X49_002538"
     CDS             complement(20029..21225)
                     /gene="kbl"
                     /locus_tag="L2X49_002538"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBN4797610.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            21439..22371
                     /gene="rfaD"
                     /locus_tag="L2X49_002539"
     CDS             21439..22371
                     /gene="rfaD"
                     /locus_tag="L2X49_002539"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBN4797611.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNKGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVMEYMAWLNRDA"
     gene            22381..23427
                     /gene="rfaF"
                     /locus_tag="L2X49_002540"
     CDS             22381..23427
                     /gene="rfaF"
                     /locus_tag="L2X49_002540"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBN4797612.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALFGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23431..24402
                     /gene="rfaC"
                     /locus_tag="L2X49_002541"
     CDS             23431..24402
                     /gene="rfaC"
                     /locus_tag="L2X49_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBN4797613.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
BASE COUNT         6143 a         6325 c         6320 g         5638 t
ORIGIN      
        1 atttttatga tgaatgctta aaacaatatt taatcgacac acatcactgt cattaatgta
       61 aagcaacata gatcaccaat aatacaatgg acaactctac cggcattgat cgctggtcgg
      121 aggaaataag gatttttaca tgaacttgaa gaagattttt ttcagtgctg tcactgtttc
      181 agttctctgc gcattaacgg gatgtgacta cattgaagaa ggcaagccag aaagtagttt
      241 attgaaacaa caagaagaac ataacaataa aattgatcta cttgaaaaac aacaagcaca
      301 gctaaagagt caactggaaa ctattcaaaa acaacaaact ggcataataa gcagtacaaa
      361 gactttaact cacgtaatta aatccgttaa agatcaacaa aacactttta tttttaccga
      421 gtttaaccca gcaaaaacga agtatttcat cctgaacaat ggttcggtgg ctttagcggg
      481 tcgagtgtta tccattgacg ccacagagaa tggcagtgtt attcatattt cactggtcaa
      541 cttattaagt acacctatct caaatattgg ttttaatgcg acatggggtg gtgaaaaacc
      601 tgtcgatgcc aaagagtttg cccggtggca acaattactt ttcaacacat caatgaaatc
      661 cacattgaaa ttattaccag gtcaatggca agacattaat ttgaccctga agggtgtatc
      721 gcccaataac ctgggatatc tgaagttagc catcaacatg gaaaatattc agttcgacaa
      781 tcttccctct gctgaaaatc ggcagaaaag aagcaaaaaa taaatgcagt tagtacgctt
      841 ataacaagga atagcgagca tcattaatga acaaaatatt taaagttatc tggaaccctg
      901 cgacagggaa ttatactgtt accagcgaaa cggcaaaaag ccgtggcaag aaatctgggc
      961 gcagtaagct gttaatttct gcgctggttg cgggtggaat gttgtcgtcg tttggggcat
     1021 tggcgaatgc cgggaatgac aacggtcagg gtgttgatta cggtagtgga tcagctggcg
     1081 acggctgggt tgctataggc aaaggggcga aagcaaatac ttttatgaac accagtggtt
     1141 ccagtactgc tgtgggttat gacgctatag ctgaaggcca atatagctct gccatcgggt
     1201 caaaaaccca tgcgattggc ggtgcatcaa tggcctttgg ggttagtgca atatcagaag
     1261 gcgatagaag tatagcactg ggtgcctctt cgtattcatt gggccaatac tcaatggccc
     1321 tcggccgtta ttcaaaagca ttgggtaaat tgtctattgc tatgggggac tcttccaaag
     1381 cggaaggagc aaacgccatt gccctgggaa atgccactaa agctactgag attatgagta
     1441 ttgctcttgg cgacaccgcc aatgcgtcaa aagcgtattc aatggcgctg ggagcaagta
     1501 gcgtcgcatc tgaagaaaac gcaattgccc tggggcgtag cagtgtagct agcggtactg
     1561 acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt gcgataggtg
     1621 ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga
     1681 ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag
     1741 gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg
     1801 atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg
     1861 ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca
     1921 gtgccaacgg tattaactct gtcgcactgg gcgcagattc cattgcggat ttagacaata
     1981 ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga
     2041 tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg
     2101 tagcaaaaag gcttggagga ggggctgcag tagatgttga tgacggtact gttacagcac
     2161 caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg ggctgcgctc gctgtacttg
     2221 atgaaaacac cctgcaatgg gaccaaacca aaggcaaata cagcgctgct catggtacta
     2281 gtagcccaac tgccagcgta atcaccgatg ttgcggatgg cacgatttca gcctccagta
     2341 aggatgcggt taacggttcc caactgaaag ctaccaatga cgatgtcgaa gccaacaccg
     2401 ccaatatcgc tactaatacc agcaacattg ccacgaatac ggcaaatatt gccaccaata
     2461 ccaccaatat caccaacctg acggattccg ttggtgacct tcaggctgat gccctgctct
     2521 ggaacgaaac taaaaaggca ttcagtgcag ctcacggcca ggataccacc agcaaaatca
     2581 ccaacgttaa agatgccgac ctgacggctg acagcactga tgctgttaac ggctctcagc
     2641 tgaaaaccac caacgatgct gtggcgacga ataccaccaa tatcgccaat aacacttcca
     2701 atattgccac taacaccacc aacatctcta acctgactga gacggtgact aatcttggtg
     2761 aggatgcgct gaaatgggat aaggacaatg gtgtattcac ggcagctcat ggcaccgaga
     2821 ccaccagcaa aatcaccaac gttaaagatg gcgacctgac gactggcagc accgatgccg
     2881 ttaacggctc tcagctgaaa accaccaacg atgccgtggc gacgaatacc accaatatcg
     2941 ccactaacac caccaacatc tctaatctga ctgagacggt gactaatctt ggtgaggatg
     3001 cgctgaaatg ggataaggac aatggtgtct tcactgcagc tcatggcaac aataccgcca
     3061 gcaaaatcac caatatcctg gacggcacag tcactgcaac cagttccgat gccattaacg
     3121 gtagccagct ttatgactta agcagcaata tcgccaccta cttcggcggc aatgcttctg
     3181 tgaatactga cggtgtgttt accggtccaa cctacaaaat cggtgaaaca aattattata
     3241 acgtctgcga tgcactggct gcgattaact cctcatttag cacgtctctc ggcgatgctc
     3301 tgctttggga tgccaccgca ggtaaattca gtgccaaaca cggtactaat ggtgacgcaa
     3361 gcgtgatcac tgatgtcgca gatggtgaaa tttcagactc cagttctgac gcagtaaacg
     3421 gctcacaact ccacggcgtg agcagttatg ttgttgatgc gctggggggt ggtgccgaag
     3481 tcaatgcaga cggcaccatc actgcgccga cgtacaccat tgctaatgct gattacgata
     3541 atgtcggtga tgccctgaat gctatcgata ccactcttga cgacgctctg ctctgggatg
     3601 cggacgccgg tgaaaatggt gcatttagcg ccgctcacgg aaaagataaa actgccagtg
     3661 taatcactaa cgtcgctaac ggtgcaatct ctgctgccag cagcgacgcg attaacggct
     3721 cacaactcta taccaccaat aagtacatcg ctgatgcgct gggtggtgac gcagaagtca
     3781 acgccgacgg caccatcacc gcaccgactt acaccattgc gaacgccgag tacaacaacg
     3841 tcggtgacgc cctggatgcg cttgatgata acgccctgct gtgggatgag actgccaatg
     3901 gcggtgctgg agcctacaat gccagccatg acggtaaagc cagcatcatc actaatgtcg
     3961 ctaatggcag tattagtgag gacagtaccg atgcagtgaa cggttctcag ttgaatgcga
     4021 cgaatatgat gattgagcag aacacccaaa ttatcaatca gctcgctggt aacaccgacg
     4081 caacctatat ccaagaaaac ggtgcgggta ttaactatgt gcgtactaac gacgacggct
     4141 tagcgttcaa cgacgccagc gcacagggtg ttggcgctac agctataggt tataactctg
     4201 tcgccaaagg cgatagcagc gtagctattg gtcagggcag ctacagcgac gttgatacgg
     4261 gtatcgccct gggtagcagc tctgtttcca gccgagtgat tgccaaaggc tcccgtgaca
     4321 ccagcataac ggaaaatggc gttgttattg gttacgacac cacggatggc gaactgctcg
     4381 gtgcattgtc tatcggtgat gacggtaaat atcgtcaaat catcaacgta gccgatggtt
     4441 ccgaagccca tgacgccgtt acggttcgtc aattgcagaa tgcgattggt gctgtagcaa
     4501 cgacaccgtc caaatacttc catgctaatt caacggaaga agattcactg gcagtgggaa
     4561 ctgactcgct ggcaatgggt gcgaaaacca tcgtgaatgg cgataaaggt attggtatcg
     4621 gttatggtgc ctacgtggac gcgaatgcac ttaacggcat tgccattggt agcaatgcgc
     4681 aagtcattcg tgtcaacagt attgcgatag gtaatggttc tacgaccact cgtggcgctc
     4741 aaaccaatta taccgcctac aacatggacg caccgcagaa ctctgtcggt gaattctcag
     4801 ttggtagtgc ggatggtcaa cgtcagatca ctaacgtcgc agcaggttcg gctgataccg
     4861 atgcggtcaa cgtgggtcag ttgaaagtaa cggatgcgca ggtttcccag aatacccaga
     4921 gcattactaa cctggataat cgggtaacga atcttgattc acgcgtcacc aatatcgaaa
     4981 acggtattgg cgatatcgtc accaccggta gcaccaagta cttcaagacc aataccgatg
     5041 gtgtagatgc cagcgcgcag ggtaaagata gcgttgctat tggttctggt tccattgctg
     5101 ccgctgacaa cagcgtcgca ctgggtaccg gttccgttgc agaggaagaa aatacaatct
     5161 ctgtaggttc ttctactaac caacgccgta tcaccaacgt agctgccggt gttaatgcca
     5221 ccgatgcggt taacgtgtcg cagctgaaat cttctgaagc aggcggcgtg cgctacgaca
     5281 ccaaagctga tggttctatc gactatagca atatcaccct cggtggcggc aacggcggta
     5341 cgactcgtat cagcaacgtc tccgctggcg tcaacaacaa cgacgcggtg aattacgcgc
     5401 agttgaagca aagcgtgcag gaaacgaagc aatacaccga tcagcgaatg gttgagatgg
     5461 ataacaaact gtctaaaact gaaagcaagt tgagcggtgg tatcgcttct gcaatggcaa
     5521 tgaccggtct gccgcaggct tacactccag gtgccagcat ggcctctatt ggtggcggta
     5581 cttacaacgg tgaatcggca gttgctttag gtgtatcgat ggtgagcgcc aatggtcgtt
     5641 gggtctacaa attacaaggt agtaccaata gccagggtga atactccgcc gcactcggtg
     5701 ccggtattca gtggtaatga tccattaagt tagtgtgact aagggcaaac gccaaattgc
     5761 ctgatgcgtt acgcgcatca ggcaagataa ggcgctccgg gtcaacaatc taagggcaat
     5821 tctctgatga ggattgccct tttctttacc agacatctcc ccccacaaga attggcccta
     5881 ccaattcttc gcttatctga cctctggttc acaattcccc aattaaaact cacatcaata
     5941 ttgccaatac ataacattta gttaaccatt cattgtcatt atccctacac aacacaattg
     6001 gcagtgccac ttttacacaa cgtgtgacaa ggagatgagc aacagactca ttacacgatg
     6061 tgcgtggact ccaggagacc tgcaatgaat ctctggcaac aaaactacga tcccgccggg
     6121 aatatctggc tttccagtct gatagcatcg ctccccatcc tgtttttctt ctttgcgctg
     6181 attaagctca aactgaaagg atacgtcgcc gcctcatgga cggtagcaat cgcccttgcc
     6241 gtggctttgc tgttctataa aatgccggtc gctaacgcgc tggcctcggt ggtttatggc
     6301 ttcttctatg gtttgtggcc catcgcgtgg atcattattg cagcagtgtt cgtctataag
     6361 atctcggtga aaaccgggca gtttgacatc attcgctcgt ctattctttc gataacccct
     6421 gaccagcgtc tgcaaatgct gatcgtcggt ttctgtttcg gcgctttcct tgaaggagcc
     6481 gcaggctttg gcgcaccggt agcaattacc gccgcattgc tggtcggcct gggttttaaa
     6541 ccgctgtacg ccgccggact gtgcctgatt gttaacaccg cgccagtggc atttggcgcg
     6601 atgggcattc caatcctggt tgccggacaa gtaacaggca tcgacagctt tgagattggc
     6661 cagatggtgg ggcggcagct accgtttatg accattatcg tgctgttctg gatcatggcg
     6721 attatggacg gctggcgcgg tatcaaggag acgtggcctg cggtcgtggt tgcgggcggc
     6781 tcgtttgcca tcgctcagta ccttagctct aacttcattg ggccggagct gccggacatt
     6841 atctcttcgc tggtatcact gctctgcctg acgctgttcc tcaaacgctg gcagccagtg
     6901 cgcgtattcc gttttggtga tttgggggcg tcacaggttg atatgacgct ggcccacacc
     6961 ggttacactg cgggtcaggt gttacgtgcc tggacaccgt tcctgttcct gacagctacc
     7021 gtaacactgt ggagtatccc gccgtttaaa gccctgttcg catcgggtgg tgcgctgtat
     7081 gagtgggtga tcaacattcc ggtgccgtac ctcgataaac tggttgcccg tatgccacca
     7141 gtggtcagcg aggctacagc ctatgccgcc gtgtttaagt ttgactggtt ctctgccacc
     7201 ggcaccgcca ttctgtttgc tgcactgctc tcgattgtct ggctgaagat gaaaccgtct
     7261 gacgctatca gcaccttcgg cagcacgctg aaagaactgg ctctacccat ctactccatc
     7321 ggcatggtgc tggcgttcgc ctttatctcg aactattccg gactgtcatc aacactggca
     7381 ctggcactgg cgcacaccgg tcatgcattc accttcttct cgccgttcct cggctggctg
     7441 ggggtattcc tgaccgggtc ggatacctca tctaacgccc tgttcgccgc gctgcaagcc
     7501 accgcagcac aacaaattgg cgtctctgat ctgttgctgg ttgccgccaa taccaccggt
     7561 ggcgtcaccg gtaagatgat ctccccgcaa tctatcgcta tcgcctgtgc ggcggtaggc
     7621 ctggtgggca aagagtctga tttgttccgc tttactgtca aacacagcct gatcttcacc
     7681 tgtatggtgg gcgtgatcac cacgcttcag gcttatgtct taacgtggat gattccttaa
     7741 tgattgtttt acccagacgc ctgtcagacg aggttgccga tcgtgtgcgg gcgctgattg
     7801 atgaaaaaaa cctggaagcg ggcatgaagt tgcccgctga gcgccaactg gcgatgcaac
     7861 tcggcgtatc acgtaattca ctgcgcgagg cgctggcaaa actggtgagc gaaggcgtgc
     7921 tgctcagtcg acgcggcggc gggacgttta ttcgctggcg tcatgacaca tggtcggagc
     7981 aaaacatcgt ccagccgcta aaaacgctga tggccgatga tccggattac agtttcgata
     8041 ttctggaagc ccgctacgcc attgaagcca gcaccgcatg gcatgcggca atgcgcgcca
     8101 cacctggcga caaagaaaag attcagcttt gctttgaagc aacgctaagt gaagacccgg
     8161 atatcgcctc acaagcggac gttcgttttc atctggcgat tgccgaagcc tcacataaca
     8221 tcgtgctgct gcaaaccatg cgcggtttct tcgatgtcct gcaatcctca gtgaagcata
     8281 gccgtcagcg gatgtatctg gtgccaccgg ttttttcaca actgaccgaa caacatcagg
     8341 ctgtcattga cgccattttt gctggtgatg ccgacggggc gcgtaaagca atgatggcgc
     8401 accttagttt tgttcacacc accatgaaac gattcgatga agatcaggct cgccacgcac
     8461 ggattacccg cctgcccggt gagcataatg agcattcgag ggagaaaaac gcatgattat
     8521 ttccgcagcc agcgattatc gcgccgcagc gcaacgcatt ctgccgccgt tcctgttcca
     8581 ctatatggat gggggtgcat attctgaata cacgctgcgc cgcaacgtgg aagatttgtc
     8641 agaagtggcg ctgcgccagc gtattctgaa aaacatgtcc gacttaagcc tggaaacgac
     8701 gctgtttaat gagaaattgt cgatgccggt ggcgctaggt ccggtaggtt tgtgtggcat
     8761 gtatgcgcga cgcggcgaag ttcaggcagc caaagcggcg gatgcgcatg gcattccgtt
     8821 tactctctcg acggtttccg tttgcccgat tgaagaagtc gcgccagcca tcaagcgccc
     8881 aatgtggttc cagctttatg tactgcgcga tcgcggcttt atgcgtaacg cgctggagcg
     8941 agcaaaagca gcgggttgtt cgacgctggt tttcaccgtg gatatgccga caccgggagc
     9001 gcgttatcgt gatgcgcatt ctggaatgag cggcccgaac gcggcaatgc gccgctattt
     9061 gcaggcggtg acgcatccgc aatgggcgtg ggatgtgggc ctgaacggtc gtccgcatga
     9121 tttaggaaat atttcggctt acctcggcaa accgaccgga ctggaagatt acatcggctg
     9181 gctggggaat aacttcgatc cgtccatctc atggaaagac cttgagtgga tccgcgattt
     9241 ctgggatggc ccgatggtga tcaaagggat cctcgatccg gaagatgcgc gcgatgcagt
     9301 acgttttggt gctgatggga ttgtggtttc taaccacggt ggccgccagc tggacggtgt
     9361 gctctcttct gcccgtgcac tgcctgctat tgccgatgcg gtgaaaggtg atatcgccat
     9421 tctggcggat agcggaattc gtaacgggct tgatgtcgtg cgtatgattg cgctcggtgc
     9481 cgacaccgta ctgctgggtc gtgctttcct gtatgcgctg gcaacagcgg gccaggcggg
     9541 tgtagctaac ctgctaaatc tgatcgagaa agagatgaaa gtggcaatga cgctgactgg
     9601 cgcgaaatcg atcagtgaaa ttacgcaaga ttcgctggtg caggggctgg gtaaagagtt
     9661 gcctacggca ctggctccga tggcgaaagg gaatgcggca tagtcgtttg agccccctca
     9721 ccctaaccct ctccctcagg gagtggggac cgttcggcgc tgtatgtact ccctcactct
     9781 gaaacgacac cgcactcttt ttttctccct cgcccctccg gggtgagggg aaaaggccgc
     9841 actgctcacc aattttctgc tatcctgccc ccgcactaag ggggcagtat gctaaacatc
     9901 gtactttacg aaccagaaat tccgccaaat actggcaaca tcatccgtct ttgcgctaat
     9961 accggctttc gtctgcatat catcgaaccg atgggatttg cctgggacga taagcgcctg
    10021 cgccgcgcgg ggctggacta tcacgagttt accgccgtta cgcgtcatca tgactatcgc
    10081 gcgttcctcg aagcagaaaa tccccagcgc ctgtttgccc tcaccacgaa aggtacgcct
    10141 gctcacagcg ccgtaagcta tcaggatggc gattatctga tgttcggccc ggaaacacgc
    10201 ggcctgccag cgagcattct tgatgccctg cccgctgaac aaaaaattcg cattccgatg
    10261 gtgccggaca gccgcagcat gaatctgtcc aatgcggtgt cggtagtggt gtatgaagcc
    10321 tggcggcagc tgggatatcc gggagcgttg ttgagagatt agttactgta tgccggatag
    10381 atgattacat cgcatccggc acgatcacag gacattagat cccatcccca tactcaaatg
    10441 tatggttaat accgttgaaa tgctggtcca tatccattga tggcttatcg ctgtctggtt
    10501 taccgacaat acgagccgga acgccagcgg cggttgtatg cggcggcacc ggttgcagca
    10561 ccacggaacc tgcgccaatc ttcgcgccgc gaccaacttc aatattgccg aggattttcg
    10621 cgcccgcgcc aatcatcaca ccttcacgaa ttttcgggtg acggtcacca ccagatttac
    10681 ccgtaccgcc aagcgtcaca gattgcagaa tcgatacgtc gttttcaatt accgccgttt
    10741 cacccacaac gatgcccgtg gcgtggtcga gcataatgcc acggccaatt tttgctgccg
    10801 ggtgaatatc gacctggaac gtcacagaaa cctggttttg cagaaagatt gccaatgcgc
    10861 gacgcccctg attccacaac cagtgaccga tgcgataggc ctgcaaggca tgaaaaccct
    10921 tcaggtataa caacggggtt gagtatttat cgactgccgg gtcgcgggta cgcaccgcct
    10981 gaatatcaca ggccgcagag gcgatcattt ccgggtcagc ggcgtaggct tcttccacca
    11041 cttcacggat agcaatagca ggcataattg gcgatgacag cttgttcgcc agcatgtagc
    11101 tcagtgcact accaaggttt tcgtgcttga gtagcgtcgc gtggtaaaaa ctggccagca
    11161 ttggctcaca gtccgccagc gttctggctt cggctttaat attgttccag acaatttcca
    11221 gttcttcaca cgacattgct tactccacac gatgagataa tgaccggccc gttctgcgcg
    11281 ggccgggtca tagcggtaac aaaggttccc tggggttagt ggctgctgcg ctcgtccttg
    11341 cgtgcacgac ctaataacgt caatgctgcc tcgcgcgcgt tttttccgca atataatact
    11401 tgataaattt cctcggttat tggcatttca acgccgaagc gatgcgccag ttcgcggact
    11461 tctttcgtat tgcggtagcc ttccaccacc tgaccaatct tctcctgcgc gctttgtaca
    11521 tccatgccct gaccgagcat catgccaaaa cggcggttac gcgactggtt gtcggtacag
    11581 gtaagcacca gatcgccaag ccccgccatg cccataaagg tggcaggatc ggcacccagc
    11641 gccgcaccaa gacgcgacat ttcagccagc ccacgggtga tcagcgccgt acgcgcattc
    11701 gcaccaaaac cgataccgtc ggacatcccc gcgccaatgg caatgacgtt tttcaccgca
    11761 ccgccaagct gcacaccgat gaaatccggg ttgctgtaaa cgcggaaact tttgccacag
    11821 tgcagcaatt gttggagatc atcggcaaag gtctggtcgg tagaggccaa cgaaatagcc
    11881 gtcggtaaac ctgccgccag ttctttcgca aacgttgggc cagagataac cgccagcgga
    11941 atttgatcgc ctaaggcctc acgcgccacg tcctgtaaca gacgtccggt ttccgcttcc
    12001 agccctttgg tcgcccacac cagacgcgca tcaggacgca tcagcggttt aatctggcgc
    12061 agcacttcac caaagacatg gctgggtacg acgacgagaa tattacggct ggctgccagc
    12121 gcagtggcga gatcgctttc aagatggagc gtatcgggaa aaggcacatc ggggagaaac
    12181 gcggcgttac agcggtcgcg ttcaagcgtt gcgatatgtt cagggtcatg gccccagagg
    12241 acaacctcgt ggccatttct tgccagggta atggcaagag cggtgccgta cgagccggca
    12301 ccgatcacag tcattgaagc attacgttgg ttcatcaggc atcctgatgt tcttcagtac
    12361 cttcgccagc ctgctgctgc aaatagttca tgaacagcgc atcgaagtta accggcgcaa
    12421 ggttcagttg cgggaatgta ccgcgggata ccatgctggt gatgcactca cgagcatacg
    12481 ggaacagaat gttcgggcag tatgcgccca ggcaatgtgc catctgggtg ccttcgatac
    12541 ccgcgatgga gaaaataccg ccctgctgaa cttcacacag gaacgcggtt tcttcgccca
    12601 gggaggccgt tacggtaaca cgcagtacca cttcgtaaac gtcatctgcc agttgggaag
    12661 atgccgtatc cagatcaagt ttaacttctg gctgccaatc tttctggaaa acgtgcggcg
    12721 cgttcggcgc ttcgaaagaa atatccttgg tataaatacg ttggatctgg aaagtcattt
    12781 cggtgttgtt ttgttctgac atgtgtagaa aacccttaag tgttgtcctt aaatactgcg
    12841 caatgtaatg ccaacgttca cacgttattt cagcagggga tccagtccac cacgtgcatc
    12901 caatgcatac aagtcatcac agccgccaat gtgctgtgcg tcaataaaaa tctggggaac
    12961 cgtggtgcga ccgctgcgtt tgatcatctc ttcacgcttg gcggcattgc catcgatcgg
    13021 caactcctga aaactcacgc ccttgctgct cagcagtgct tttgcacgat ggcaatacgg
    13081 gcaggtttct ttggtataga tttcaacatt ggccataatt tagctcctgt ttttttaccc
    13141 tgaaatttca tgttgcaggg cggctgcaaa atggagtgtc cccagaagct tataacaata
    13201 agtcgctggg ggcctaattg cagctaacgg cctgcatcat gaaagacgac aggtaaatta
    13261 tttgccgcgc accaaaggca agttttcgcc agcccagcca gcgacgcctt ctttcagcac
    13321 gaatacttgc gcaaaaccag ctttcgtcag tgcgtttgca ggctcctggc actgcatgcc
    13381 agaaccgtct accacgataa ccggtttgtc tttgtgcttc tcaagctcac caacattgtt
    13441 ggctttgatt tcgctcggca acagattgat agaacctgcg atatggcctt tacggaagtc
    13501 atcacgctga cgtaaatcca caaccacagc gtcttctttg ttgatcagac gcgtagcttc
    13561 accacgagta atcaccttca ctttcgaggt caggctctta aacgtagtca caagaaccgc
    13621 caccagtaac gcgatccagg cgatactcag tatgggatga cggccaacaa attgcataat
    13681 ttcttgcatg gggggtaaca actcccgacg tagtgattaa aaaaaccagg aaaggagtat
    13741 acctgcgcgg tgcggcaaat acagccagcg cgttaactgg aatgcaattt tgcggggcac
    13801 gacgaaaaaa aagcgcaaat ctggcatcgg cttgccgcga gcggtcgtat attttgatct
    13861 tcagaggcta ttttatcgat tcagctgtag taaaattacg caaattttga ctcttgagta
    13921 tgaggttgtc gcaatgtcgg tttctaaaaa acctatggta ctggtgattc tggatggcta
    13981 tggctatcgt gaagaacagc aggataacgc catttttagt gctaaaaccc cggtaatgga
    14041 tgcactgtgg gccaatcgtc cgcataccct gatcgacgct tccggtctgg aagtcggtct
    14101 gcctgaccgt cagatgggta actccgaagt aggccacgtt aacctgggtg ccggccgcat
    14161 cgtgtatcag gacctgactc gtctggacgt tgaaatcaaa gatcgcgctt tctttgctaa
    14221 tccagtgctg actggcgcgg tagataaagc gaaaaacgca ggtaaagcag tacacattat
    14281 gggtctgctc tccgcaggcg gcgtacacag ccacgaagat cacatcatgg cgatggtaga
    14341 actggcagct gaacgcggcg cagaaaaaat ctacctgcac gcattccttg acggtcgcga
    14401 caccccgccg cgcagtgctg aatcctcgct gaaaaaattt gaagagaaat ttgctgcgct
    14461 gggtaaaggc cgcgtagcgt ccatcattgg tcgttactac gcgatggacc gcgataaccg
    14521 ttgggatcgc gttgaaaaag cctatgacct gctgactctg gcacagggtg agttccaggc
    14581 cgatactgcc gttgctggtt tgcaggcagc ttatgctcgc gacgaaaacg acgagttcgt
    14641 gaaagcgacc gttattcgtg cagaaggtca gccagatgcg gcaatggaag acggcgatgc
    14701 gctgattttc atgaacttcc gtgctgaccg cgcgcgcgaa atcactcgtg cattcgtaaa
    14761 cgcagatttc gacggcttcg cgcgtaagaa agtggttaac gtcgatttcg tgatgctgac
    14821 cgaatacgcc gctgacatca aaactgcggt tgcttaccca cccgcttccc tggttaacac
    14881 cttcggcgag tggatggcga aaaacgacaa aactcagttg cgtatttccg aaaccgaaaa
    14941 atatgcccac gttactttct tcttcaacgg tggcgtagaa gagtcgttca aaggcgaaga
    15001 tcgcattctg atcaactcac cgaaagtggc tacctacgat ctgcaaccgg aaatgagctc
    15061 cgcagagctg accgaaaaac tggttgcggc catcaagagc ggcaaatacg acaccattat
    15121 ctgtaactat ccgaacggcg acatggtagg tcacaccggg gtgatggaag cggcggttaa
    15181 agcggttgaa gcactggatc actgcgtgga agaagtcgcg aaagcggttg agtccgtggg
    15241 tggacaactg ctgatcaccg ctgaccacgg taacgctgaa cagatgcgcg atccggcaac
    15301 gggtcaggca cacacggcac acaccaacct gccagttccg ctgatttacg ttggtgataa
    15361 gaacgtgaaa gcggttgcag gcggcaaact ttctgacatc gcgccgacca tgttgtcgct
    15421 gatgggtatg gaaatcccgc aagagatgac tggtaagccg ctgttcatcg tggaataatc
    15481 cctccccatg aggggaaagg cgattaatac catgacacgg gccgtgaaac cgcgcaggtt
    15541 tgcaatcagg cccatcatct acgccagcgt tcttagcgct ggcgtattgt tgtgcgcctt
    15601 ttccgcccac gcggatgagc gtgaccaact caaatctatt caggccgata tcgccgcgaa
    15661 agagcgcgcg gtacgccaaa agcaacaaca acgcgcaagc ctgctcgcac aattgaaaaa
    15721 gcaggaagaa gcgatctctg aagccacccg taagctgcgc gaaacgcaaa acacgctcaa
    15781 tcaactcaat aaacagattg atgagatgaa cgcgtcgatt gccaaactgg agcagcaaaa
    15841 agccgcccag gagcgcagcc tcgccgcaca actggatgcc gcattccgtc agggcgagca
    15901 taccggtatt cagctaattc tcagcggtga agaaagccag cgtggacagc gtttacaggc
    15961 ttatttcggc tatctcaacc aggcgcgaca agaaaccatt gcccagttga agcaaacgcg
    16021 tgaagaagtc gccatgcagc gtgctgaact ggaagagaaa cagagcgagc aacaaacgct
    16081 gttatatgaa cagcgcgccc aacaggcgaa actgactcag gctctgaacg agcgtaaaaa
    16141 gacgctggcg gggctggaat cttccatcca gcaaggtcag cagcagctaa gtgaactgcg
    16201 tgccaacgaa tcccgtctgc gtaacagcat tgcccgtgcg gaagccgcgg cgaaagcgcg
    16261 tgctgaacga gaagcgcgtg aggcccaggc ggttcgcgac cgccagaaag aagcgacgcg
    16321 caaaggcacc acctataaac cgaccgaaag cgaaaaatcg ctgatgtccc gaactggtgg
    16381 cctgggtgcg ccgcgtggtc aggcattctg gccggttcgc gggccgacgc tgcatcgcta
    16441 tggtgaacag ctacagggcg aactacgctg gaaaggaatg gttatcggtg cctctgaagg
    16501 tactgaagtt aaagcgattg ccgacggtcg ggtgattctg gctgactggc tgcaaggtta
    16561 cggtctggtg gtggtggttg agcacggtaa aggcgacatg agtctttacg gctataatca
    16621 gagcgcactg gtgagcgttg gttcgcaggt tcgcgcgggc cagccaattg cactggtggg
    16681 cagcagtggc ggtcagggtc ggccttcact ctatttcgaa attcgccgcc agggtcaggc
    16741 ggtcaatcca cagccgtggt tgggaagata agttttgttt ccatttcgtc gtaacgttct
    16801 tgcatttgcc gctctgttgg cgctctcctc ccccgtactt gctggcaaac ttgccatcgt
    16861 cattgatgat tttgggtatc gcccgcacaa cgaaaaccag gtgctggcga tgccctccgc
    16921 tatctccgtc gctgtattac ccgattcacc gcacgccaga gagatggcga ccaaagcgca
    16981 taacagcggg cacgaagtgt tgattcatct cccgatggca ccactgagta aacagccgct
    17041 ggagaaaaat acgctgcgcc cggagatgag cagcgacgaa attgagcgca ttattcgtag
    17101 tgcggtcaat aacgtgccct atgccgtggg gatcaacaac cacatgggta gcaagatgac
    17161 ctctaacctg tttggtatgc agaaagtgat gcaggcactg gagcgttaca atctttactt
    17221 cctcgacagc gtaaccatcg gtaataccca ggcgatgcgc gccgcgcaag gtactggcgt
    17281 gaaggtgatc aaacggaagg tgttcctcga cgattcgcag aacgaagcgg acatccgtgt
    17341 gcaatttaat cgcgcaattg atctggcgcg tcgcaacggt tcaaccattg ccattgggca
    17401 tcctcaccct tcaacggtac gcgtgctaca acagatggtt tataacctgc cgccagacat
    17461 tacgctggtg aaagccagca gcttgctgaa tgaaccgcag gttgatactt ctacaccgcc
    17521 gaaaaacgct gtgcctgatg caccgcgtaa tccgttccgt ggcgtgaagc tgtgcaaacc
    17581 gaagaaaccg atagaacctg tttatgctaa tcgcttcttt gaagtattaa gcgaaagcat
    17641 cagccagagc acgctgatcg tttacttcca gcatcagtgg caaggctggg gcaaacagcc
    17701 cgaagcggcg aagtttaacg ctagcgcaaa ttaaaggcgc tacgcgtaat gcgatggtgc
    17761 gaccgcgttt tgtcgcgcca ttgccataag cggaaagacc acagtaacgc ctggtaaccg
    17821 accttcacgc tgcgcacatt ggtaatcagg cgcttataca taccggaagt aaatatctcg
    17881 gcaatcatcc gttggcgggt aagaatatcc ggctctttgc gcaccgcatg gcaaacacgc
    17941 aatgcttcgt aagttatttg ctgatgaaat tccggataaa tcgtaatttt gtcggcataa
    18001 tttcgattta atttctccag caggcgggta atcttaatat agtgacgttg ataattaagg
    18061 tttttattcc cttgtctatg caaccgactc actgacgtat tatgcagata atatttatat
    18121 aatgattgct cggtatatcg cgcacgcagc gcgttaaaca tgaattctgt tgtccagaca
    18181 atatcctgat gatgtaatcc ggcaataaat ttaatgttat ttttaacaat aacatcacgg
    18241 cgataaaccc ccatccagac aacgtgagtc caacggcgcg aagaaagccc catccgcagc
    18301 cagtccgggc cggttaatac gccggttgag cgaaggcgat cgctggggat ggattgccag
    18361 gtttctcccg tttcacgaaa acaccagtca gcgttgcact gcgccacgtc gaggtcatcc
    18421 tctaacgcca tggtcatcag cgtttcgtac atggtgggat agacctcatc gtcagcatcg
    18481 acaaaagcga catatttgcc cgtcgccacc tcgatcccac gattacgcgc taccgatgcg
    18541 ccagcattcg cctgatgcaa caaacgaacg tgcggatagt tttctgcgta atgctttgct
    18601 atttcaacag aattatccgt tgaaccatcg ttaataataa tgatttccag agcagtccag
    18661 gtttgcgtaa ttaaagattc catacaagtg cggaaatcat cgcccgcatt atataacgga
    18721 ataataacac taagtttatt ggtgctgttc atcataaatt accgacgacc tctgaagtta
    18781 tcctgacgtt ttacaggctt tgtattaaga aactattaag cctgtgagat attattttta
    18841 caggtgagag agtcacttca gggtagagag ataatgagag cagctatata agagatcagc
    18901 gcaattacga tatctttatt acactgacag gattttacat atttaacgga ttatcctcag
    18961 gcaaaaaggc tggaatacca gcccttgttc gtgttaatcc caactcagaa ttactttccc
    19021 ggactggccc gaacgcatag cgtcaaagcc cttctggaaa tcatcgatag agaaacgatg
    19081 ggtaatgatc ggcgaaagat cgaggccaga ctgaatcagc gccgccatct tgtaccaggt
    19141 ttcaaacatc tcacgaccgt aaataccttt aatgaacaag cctttaaaga tcactttggt
    19201 ccagtcgata gacatatcag acggcggaat acccagcatc gcaatacggc cgccgtggtt
    19261 catggtatca agcatggtac gaaacgctgg cggcgcaccg gacatttcca gaccgacatc
    19321 aaaaccttcg gtcatgccta actccgccat cacgtcattg agattttctt tggcgacgtt
    19381 aaccgcacgg gtgataccca ttttacgcgc cagctcaagg cggtattcgt taacatcagt
    19441 gatcaccaca ttgcgtgcac caacgtgttt cgccaccgcc gctgccataa taccaatcgg
    19501 gcctgcacca gaaaccagca catcctcgcc aaccagatcg aacgacagcg cggtatgcac
    19561 ggcattaccg aagggatcaa aaatggaagc caggtcgtcg gagatattgt cgggaatttt
    19621 gaaggcgttg aacgccggaa tcaccagata ttcggcgaag cagcccgggc ggttaacgcc
    19681 gacgccgatc gtgttgcggc acagatgggt acgaccgcca cgacagttgc gacagtgacc
    19741 gcaggtgata tggccttcgc cagaaacgcg atcgccaatc ttgaagccct tcacttcctg
    19801 accaatacct accacttcac cgacatattc atggcccacg accatcggca ccgggatggt
    19861 tttttgcgac cactcatccc agttatagat gtgaacgtca gtcccgcaga tggctgtttt
    19921 acggatttta atcagcagat cgttatgccc gagttccggt acaggaacgt cggtcatcca
    19981 gatgccctct tccgctttca gtttggataa cgctttcatc tcacatcctc aggcgataac
    20041 gcccagttgt ttaccaatac gcgtgaatgc ttctactgca cgcgtaattt gctcaggggt
    20101 atgcgccgca gacatctggg tacgaatacg cgcctgacct ttcggaacga ccggatagaa
    20161 gaaaccggtg acgtaaatgc cctctttttg cagctcacgg gcaaatttct gcgccactac
    20221 cgcatcacca agcatgaccg gaataatggc gtgatcggct cccgccaggg taaagcccgc
    20281 cgccgacatt tgctcacgga actgacgcgc gttcgcccac agacggtcac gcaactcgct
    20341 acccgcttca accatctcca gcactttgat ggaagcggcg acaatcgctg gtgccagcga
    20401 gttggagaac aggtacggac gagaacgctg gcgcagccac tcaaccactt ctttgcgcgc
    20461 cgcggtataa ccaccagaag ccccgcccag cgctttaccg agcgtgccgg tgatgatgtc
    20521 gacacgcccc atcacatcgc agtattcatg ggaaccacga ccattttcac cgacaaaacc
    20581 gaccgcgtgg gagtcgtcta ccatcaccag ggcatcgtat ttatctgcca gatcgcaaac
    20641 gcccttcaga ttggcaatca cgccgtccat cgagaacaca ccatcggtgg caatcagcac
    20701 atgacgcgca ccggcttcac gcgcttcttt cagacgagct tcaagctcct gcatatcgtt
    20761 gttggcatag cgatagcgtt tagctttaca cagacgcaca ccatcaataa tagacgcgtg
    20821 gttcagtgcg tcggagataa tggcgtcttc cgcgcccagc agcgtttcaa acaggccacc
    20881 gttagcatca aagcaggaag agtagagaat cgcatcttcc atccccagga atgccgccag
    20941 tttttgttca agctctttat ggctgtcctg agtgccgcaa ataaaacgca ccgaagccat
    21001 gccgaaaccg tgagaatcca ttcccgcctt tgctgccgca atcagatccg gatgattcgc
    21061 cagcccgaga tagttgttgg cacaaaagtt aatgacgtgg cttccatcag ccacggtgat
    21121 atctgcttgc tgcgcagacg taataatgcg ctcttcttta aacaaccctt ccgcccgtgc
    21181 ggtttccaga tcgttggtta actgctgata aaattctcca cgcattgcga ttctccagac
    21241 agggcaaatt ccagcacata ttacccaaac ttataggtcg cgacgagata acgcgttaac
    21301 acttctgcaa aattcaggat aaataacgtc catcaatctc acttttggcg aatggctgaa
    21361 gccaacgccg tgtagtgata tgatagaagt attcgtgtct gagattgtct ctgactccat
    21421 aattcgaagg ttacagttat gatcatcgtt accggcggcg cgggctttat cggcagcaac
    21481 atcgttaagg ccctgaatga taaaggcatc accgatattc tggtggtgga caacctgaaa
    21541 gacggcacca agtttgtgaa cctggtggat ctggatatcg cggactatat ggataaggaa
    21601 gacttcctga tccagattat ggctggcgaa gagttcggcg atgtcgaagc gattttccac
    21661 gaaggcgcat gctcttccac caccgagtgg gacggcaagt atatgatgga taacaactat
    21721 caatactcca aggagctgct gcactactgc ctggagcgcg aaatcccgtt cctgtacgct
    21781 tcttccgcag ccacctacgg cggacgcacc tccgacttta ttgaatcccg cgagtacgaa
    21841 aaaccgttga acgtctacgg ttactcaaaa ttcctgtttg atgaatatgt tcgccaaatc
    21901 ctgcctgaag cgaactcgca gattgttggc ttccgctatt tcaacgttta tggaccgcgt
    21961 gaaggccata aaggcagcat ggcgagcgtc gctttccatc tcaacactca gcttaacaaa
    22021 ggtgaatcac cgaagctgtt tgaaggtagc gagaacttca aacgtgactt cgtctatgtg
    22081 ggcgacgtgg cagatgtaaa cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac
    22141 ctcggtactg gtcgtgcgga atccttccag gccgtagcag atgctactct ggcttatcac
    22201 aagaaaggcc agatcgaata cattccgttc ccggataagc tgaaaggccg ctaccaggcg
    22261 ttcactcagg cagatctgac aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc
    22321 gttgctgaag gtgtaatgga atacatggcc tggctgaatc gcgacgcata agagctctgc
    22381 atgaaaatac tggtgatcgg cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc
    22441 tatcgcacgc tccaggcgcg ctatccccag gcgataatcg atgtgatggc accggcatgg
    22501 tgccgtccat tattatcgcg gatgccggaa gttaacgaag ctatccctat gcctctcggt
    22561 cacggagcgc tggaaatcgg cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc
    22621 tacgaccgcg cctacgtctt accaaactcc ttcaaatctg cattagtgcc tttcttcgcg
    22681 ggtattcctc atcgcactgg ctggcgcggc gagatgcgct acggtttact caacgatgta
    22741 cgcgtgctcg ataaagaagc ctggccgcta atggtggaac gctatgtcgc gctggcctat
    22801 gacaaaggca ttatgcgtac cgcacaagat ctgccgcagc cattgttatg gccgcagttg
    22861 caggtgagcg aaggtgaaaa atcatatacc tgtaatcaat tttcgctttc atcagaacgt
    22921 ccgatgattg gcttttgccc gggtgcggag tttggtccgg caaaacgctg gccacactac
    22981 cactatgcgg agctggcaaa gcagctgatt gatgaaggtt atcaggtggt tctgtttggc
    23041 tctgcgaaag atcatgaagc gggcaatgag attcttgccg ctttgaatac cgagcagcag
    23101 gcatggtgtc ggaacctggc gggggaaaca cagcttgatc aagcggttat cctgattgca
    23161 gcctgtaaag ccattgtcac taacgattct ggcctaatgc acgttgcggc ggcgctcaat
    23221 cgtccgctgg ttgccctgtt tggtccgagt agcccggact tcacaccgcc gctatcccat
    23281 aaagcgcgcg tgatccgtct gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg
    23341 gagggttatc accagagctt gatcgacatt actccccagc gcgtactgga agaactcaac
    23401 gcgctattgt tacaagagga agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg
    23461 atgggcgatg ttctccatac gttgcccgca ctcactgatg cccagcaggc aatcccaggg
    23521 attaagtttg actgggtggt ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc
    23581 gttgagcgag ttattcctgt ggcaatacgt cgctggcgta aagcctggtt ctcggccccc
    23641 ataaaagctg aacgcaaagc gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt
    23701 atcgatgctc aggggctggt aaaaagcgcg gcgctggtga cgcgtctggc gcatggcgta
    23761 aagcatggat tggactggca aaccgctcgc gaaccgttag ccagcctgtt ttacaatcgt
    23821 aagcatcata ttgcaaaaca gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa
    23881 agtctgggct atagcaaacc gcaaacccag ggcgattatg ctattgcaca gcatttcctg
    23941 acgaacctgc ctacagatgc tggcgaatat gccgtatttc ttcatgcgac gacccgtgat
    24001 gataaacact ggccggaaga acactggcga gaattgattg gtttactggc tgattcagga
    24061 atacggatta aacttccgtg gggcgcgccg catgaggaag aacgggcgaa acgactggca
    24121 gaaggatttg cttatgttga agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg
    24181 ctggccgggg ctaaatttgt agtgtcagtg gatacggggt taagccattt aacagcggca
    24241 ctggatagac ccaatatcac ggtttatgga cctaccgatc cgggattaat tggtgggtat
    24301 gggaagaatc agatggtttg tagggctccg gggaatgagt tgtctcaatt gacagcaaat
    24361 gctgttaagc ggttcattga agaaaacgct gccatgattt aaattacaac tctttaatag
    24421 ataatt
//
LOCUS       DAFNBJ010000068        24339 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000068 DAFNBJ010000000
VERSION     DAFNBJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.068"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(139..1020)
                     /gene="prmA"
                     /locus_tag="L2X49_002542"
     CDS             complement(139..1020)
                     /gene="prmA"
                     /locus_tag="L2X49_002542"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBN4797614.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1032..2483)
                     /gene="panF"
                     /locus_tag="L2X49_002543"
     CDS             complement(1032..2483)
                     /gene="panF"
                     /locus_tag="L2X49_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBN4797615.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLVAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2473..2715)
                     /locus_tag="L2X49_002544"
     CDS             complement(2473..2715)
                     /locus_tag="L2X49_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBN4797616.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEIACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2824..4173)
                     /gene="accC"
                     /locus_tag="L2X49_002545"
     CDS             complement(2824..4173)
                     /gene="accC"
                     /locus_tag="L2X49_002545"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBN4797617.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4184..4654)
                     /gene="accB"
                     /locus_tag="L2X49_002546"
                     /gene_synonym="fabE"
     CDS             complement(4184..4654)
                     /gene="accB"
                     /locus_tag="L2X49_002546"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBN4797618.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5632..6606)
                     /locus_tag="L2X49_002547"
     CDS             complement(5632..6606)
                     /locus_tag="L2X49_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBN4797619.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEMLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6758..8698
                     /gene="csrD"
                     /locus_tag="L2X49_002548"
     CDS             6758..8698
                     /gene="csrD"
                     /locus_tag="L2X49_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBN4797620.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9003..10046
                     /gene="mreB"
                     /locus_tag="L2X49_002549"
     CDS             9003..10046
                     /gene="mreB"
                     /locus_tag="L2X49_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBN4797621.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10112..11215
                     /gene="mreC"
                     /locus_tag="L2X49_002550"
     CDS             10112..11215
                     /gene="mreC"
                     /locus_tag="L2X49_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBN4797622.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11215..11703
                     /gene="mreD"
                     /locus_tag="L2X49_002551"
     CDS             11215..11703
                     /gene="mreD"
                     /locus_tag="L2X49_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBN4797623.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11712..12305
                     /gene="yhdE"
                     /locus_tag="L2X49_002552"
     CDS             11712..12305
                     /gene="yhdE"
                     /locus_tag="L2X49_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBN4797624.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12295..13764
                     /gene="rng"
                     /locus_tag="L2X49_002553"
     CDS             12295..13764
                     /gene="rng"
                     /locus_tag="L2X49_002553"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBN4797625.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13832..17632
                     /gene="yhdP"
                     /locus_tag="L2X49_002554"
     CDS             13832..17632
                     /gene="yhdP"
                     /locus_tag="L2X49_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBN4797626.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17788..19233
                     /gene="tldD"
                     /locus_tag="L2X49_002555"
     CDS             17788..19233
                     /gene="tldD"
                     /locus_tag="L2X49_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBN4797627.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19361..20290)
                     /gene="aaeR"
                     /locus_tag="L2X49_002556"
     CDS             complement(19361..20290)
                     /gene="aaeR"
                     /locus_tag="L2X49_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBN4797628.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20473..20676
                     /locus_tag="L2X49_002557"
     CDS             20473..20676
                     /locus_tag="L2X49_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBN4797629.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20684..21616
                     /gene="aaeA"
                     /locus_tag="L2X49_002558"
     CDS             20684..21616
                     /gene="aaeA"
                     /locus_tag="L2X49_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBN4797630.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21622..23589
                     /gene="aaeB"
                     /locus_tag="L2X49_002559"
     CDS             21622..23589
                     /gene="aaeB"
                     /locus_tag="L2X49_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBN4797631.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFDQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23681..23953
                     /locus_tag="L2X49_002560"
     CDS             23681..23953
                     /locus_tag="L2X49_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBN4797632.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24009..24272)
                     /gene="yhcN"
                     /locus_tag="L2X49_002561"
     CDS             complement(24009..24272)
                     /gene="yhcN"
                     /locus_tag="L2X49_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBN4797633.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5760 a         6133 c         6610 g         5836 t
ORIGIN      
        1 aaatttgagt aagttctcaa tttttcttta tgaatgaaaa ttttggcacg caaatttgcg
       61 taaataatcg gcaaatttac gtgccttttg tgagcttgct cgcacttcgc cccgcgtcac
      121 cctacggcga tgcgaaggtt aattcttacg accggtaatg cggcaccact cttctttttc
      181 cacgaccggg tccagtgcga agctgtcgac ataagcttca caaacgctct ctgcctggct
      241 tgccagaata ccggaaaggc ccagcaaacc gcctgaaacc ggcaggacgc tgattaacgg
      301 tgccagttca cgtaatgggc ctgcaaggat gttagcgacc accacgtcgg ctttcatttc
      361 ttctggctga tctttcggta agtagagttc cagacggtca gaaacgccat tacgttcggc
      421 gttgtcgcgg ctggcctgaa tcgcctgcgg atcgatatca ataccaatgg cttttgctgc
      481 acccagtttc agcgccgcga tcgccagaat gccggaacca cagccaaagt cgatgactgt
      541 tttaccggtt aaatcgaggc tgtcgagcca ttgcaggcac agagaggtgg ttggatgggt
      601 acccgtacca aacgccagcc ctggatctaa catcacgttg acggcgtttt cgtccggcac
      661 atcacgccag ctagggcaga tccacagtcg ttcaccaaag cgcatcgggt ggaaattatc
      721 catccattcg cgctcccagt ctttatcttc tagttgttcg attttatgcg cgaagcctgc
      781 gccgagcagc ggatggtttt ccagaatcgc caccacgtcg ttcatatcgg tttcagcgtc
      841 gaacagacca atcacatcgg tgtcgcccca caggcgcgtt tcgcccggca gcggctcaaa
      901 tactggcgta tcgtgggtat cctgaaaagt gatagaaacg gcacccgctt ccatcagcgc
      961 atcgctaaga tcttccgcgt tcgcgccggt ggtgttcagt ttcagttgga tccaaggcat
     1021 ggcaaaactc tttatttatc agtagtcaaa acggtagctt gcgggacgga tgtaccgaaa
     1081 cggtttccga ccaggaaagc caccaaactt agtagtaacg agggcacgat agggtggaag
     1141 cccaggtact gaatattcag cgtcgcgagt acggcataca gcacgccgcc aacgatcatc
     1201 gcacttagcg cgcctttggc gttggcgcgt tcccagtaaa gacccagcac cagcggccac
     1261 aggaaaacgg cttccagccc accgaaggcc agcaaattca gccagatgat catttctggc
     1321 ggcttccagg cggcaagcag cagcaacgcg ccgagaacta acgtaattac cgccgacatc
     1381 cgcttcagac gcgtctcgtt ttgcatttga tccggacgga tattcagata gagatcttta
     1441 atgatcgtag cggaactttg tagcaattgg gcgttaattg tcgacatgat cgcagccata
     1501 ggtgcagcca gaaagatccc ggcagcaaac ggtggcagca cttttaccat taacgtcggg
     1561 atcaccagat ccggtacggt gagatcgggg atcactgcac gacctaacgc tccggccagg
     1621 tgcataccga acatcaggat tgcgactaca atcgtaccga tgatgatccc ccggtgtact
     1681 gctttgctgt ctttataaga gatacagcgc accgcagtat gcggcaggcc aatcacgcca
     1741 aagcacacca gtacccagaa cgacgtcata aaggcaggcg acagaatatc gtcagcgcct
     1801 tgtggcgtaa ccagttgcgg atcgatggtt tgcaaggtct gtactgcgtt gcttaagccg
     1861 ccagcggcat gtaccacgcc aataagcagc acaacggtgc caatcagcat cacaagccct
     1921 tgcatggtgt cgttcagcac gctggcgcga aagccgccaa aggcggtata caacgcaatg
     1981 ctgataccga aaatcagcag cccggtttca taaggaatac ccgccgcggt ttccagcagg
     2041 cgcgcaccgc cgataaactg cacggtcatt gccccaacga acgcaaccag caaactcaaa
     2101 ctcgccagcc acaccagaag acgactctgg tagcgggcaa acagcatatc gttcagcgtc
     2161 actgcattgt agcggcgcgc aagaatcgcg aacttcttgc cgagaatacc gagcgaaagc
     2221 cagactgcag gaagctgaat catcgccagc aatacccaac ccaggccgta tttataagca
     2281 gctccaggcc cgccgataaa cgaactggca ctgatatagg tcgcggtgag cgtcatcgcc
     2341 agcacaatac cgcccataga gcggctgccg aggaaatact cattaaggaa ggtgccggtg
     2401 ctccgtttac gcatcgcata aaccgagata ccgaacacca ccaccagata ggcgaccagc
     2461 ggtagaatta cttcaagctg catcgtcatc ctccagtggg atatcgcgat agataaattt
     2521 caccatcgcc cagcacagtc caataaacag tagcggcgtc aggatgcagg ctatctcaaa
     2581 ccagcgcgga aagccggtaa aaccgggggc aacgccaggt aagtaagcgg ctactaacca
     2641 aactgccaga tacaaaaggg tcagccccag cgcccagcgc gcctctttat gggcctgaac
     2701 aaaacgagtg tccatttttt gtccctgatg ggtgaagaaa gcgaggattg taccttatgg
     2761 gggttgtcga tccccagtaa taaaaaaggc cggaaaatcc ggccttttga cgcttttgca
     2821 gtcttatttt tcctgaagac cgagtttttt ctccagatag tggatgttag tgccaccatg
     2881 ctggaagttc tcgtcattca tgatgcggat ctgcagatca acattggttt tgataccgtc
     2941 gatgatcagc tcctgcagcg cattcttcat gcgggcaatc gccacgtcac ggttttcacc
     3001 gtagcaaatc agcttaccga tcattgagtc atagtacggc ggtacggtgt agcccgcgta
     3061 gatatgagac tcccaacgta cgccaaaacc gccaggtgcg tggaaacggg tgattttgcc
     3121 tgggcttggc aggaaggtgt tcggatcttc ggcgttgata cgacattcca ccgcatggcc
     3181 gcgaacgtga acttcttctt gcttgatcga cagcggttga ccggcagcga tacgcagctg
     3241 ttctttgatc aggtcaacgc cggtgatcat ttcggtaacc gggtgttcta cctgaatacg
     3301 ggtgttcatt tcgatgaaat agaactcgcc gttttcgaac aggaactcga aagtacctgc
     3361 accgcgatag ccgatatcaa cacacgcttt agcgcaacgt tcgccgatgt agcgacgcag
     3421 ttccggggta atgcccggtg ctggcgcttc ttcgaccact ttctggtggc ggcgctgcat
     3481 ggagcagtca cgttccgcca gatagatagc gttgccctga ccgtcagcca gtacctgaat
     3541 ctcgacgtgg cgaggatttt ccaagtattt ctccatgtaa accatatcgt tgctgaaagc
     3601 agctttcgct tccgcacggg tcatggagat ggattgtgcc agttcagcgt cgccgcgcac
     3661 tacgcgcata ccgcgaccgc cgccgccgcc ggaggctttg ataatcaccg gataaccaat
     3721 gcgtttagca atggcgcggt ttttatccat atcgtcgccc agcgggccgt cagaaccagg
     3781 tacgcaagga acgcccgctt ttttcatcgc cgcgattgcg gatactttgt cgcccatcag
     3841 gcgaatggtt tctgctttcg ggccgatgaa gataaagccg gagcgttcaa cctgctcggc
     3901 aaagttggcg ttctcggaga ggaagccgta acccggatgg attgctactg cgccggtgat
     3961 ttcagcggcg ctgatgattg ccgggatgtt cagataactt tttactgacg gagcagggcc
     4021 aatacagacc gtttcatctg ccagtaatac gtgttttaga tcgcgatccg cgctggagtg
     4081 cacagcgaca gtcttgatgc ccagttcttt acaggcacga agaatacgca atgcaatctc
     4141 gccgcggttg gcaataacaa ttttatccag catgttcgcc tcgttactcg atgacgacca
     4201 gcggctcgtc aaattctacc ggttgtccac tttcgaccag aattgctttc acggtaccgg
     4261 atttgtccgc ttcgatctgg ttcatcattt tcatggcttc aacgatgcac agggtatcgc
     4321 ccacgttgac tttctgaccc acttcgatga acgcttttgc gtccgggctt ggggtgcggt
     4381 agaaagtacc aaccatcggg gaacgtacga tgtgaccact gatttccgct gctgctggcg
     4441 cttccatgga aggaacggtc gccggagcgg ctgcgttaga ttgagctggc tgctgcatca
     4501 ttggtgcagc gtaagcttgt tgcatcacag ggaaacttgc ggcaggagct gcacggctaa
     4561 tgcgtactga ctcttcgcct tcagaaattt ccagttcgga gatgcctgat tcttcaacca
     4621 gctcgatcag ttttttaatc ttacgaatat ccatgagtgg gttccgtact ctttgtttag
     4681 tgtgattgtg acaggcgttt caccgccgtc tgtaaagcgt atgccccgac agctgtatgc
     4741 atagcgataa attccagcag gccgggacgc cggtctattt tgccccagag tgtcaatgtt
     4801 agacttgacg gacattgtgc agcgatctgc tgctatcctc cggcaaaaaa caaaatatac
     4861 cttcgttgcg ccaatgtcac cttttccgca gcgcaaaaac tgcgtcaggg aggacgaggt
     4921 cgcacattat aacgatttcg tagcaattgg cagctaaata ctggtcttat cagggaagat
     4981 aatcaacagc taacatgtaa ataaccttca acaccgtgta atttgcaaca agccgcacaa
     5041 ttcacgaaat tagcgccatc atcgacggaa cttcttataa cgtaaggtga aaaactgtaa
     5101 agccagccct gtgaagatga ggggctgcaa tgagataatc ttcacagacc acaaataatg
     5161 tagggatgcc aggatcgcga caagatagac gacattacgc agcggttgct gaggattacc
     5221 caattttcac tgcatcgtct ggatagacgt gaatgctaaa gcacgcaaaa tcaaccgact
     5281 gacaatgctc agcattaaat aggtttgagt ggttaatttg tagtcaaata aaatcagatt
     5341 attcacccca tggtaccaga aataagtaag gggctaagcg tagcgtcgcc caggcaaagc
     5401 accataaacc taacaggcga cggaggcgta tcaataatag ctgtttagtg gagtatcaat
     5461 gcgcatcaat ttgtggtcag tcgaaatcat gactttacag tttggatgat gttgtataac
     5521 tttctgacca aggtgttcga cgaaagctgt ccggctaacc gcttttcccg ttaaccgatt
     5581 gaataatgat ttcaaaatga gttccctctc ttattattcc ctgctaaatg gttagttaac
     5641 cttcaccagc gtgcgaccct ggatctggtt attaatgatg gcctcggcaa agttcggtgc
     5701 ctctgacaga gatatctctt ttgccgcctg ggtatagaat gattccggta aatcggcgac
     5761 cagtcgctgc caggcttgtg cgcggcgttc tggtggcgtc attactgaat ccaccccttg
     5821 caaacggaca ttacgcagaa taaatggcat gaccgtggtt ggcagagtaa aaccacccgc
     5881 cagaccacag gcagccacgc agccgccgta attcatttgc gccagcactt ttgccagcac
     5941 tttgtcgcca acagtgtcaa ttgccccagc ccagacttgt ttttccagag gacgggattc
     6001 ggcaaactca tcacgaggga gaatacgact ggcacctaaa cttttcagat attcatgggt
     6061 actttcgcga ccggaaacgg caacgacctg ataacccaac ttatgcagca gcgccacggc
     6121 ggtactgccg acgccaccac tggcacccgt cacgacaatc tccccgtcct gcgggcgaac
     6181 accggcatct tccagcgcca tcacacacag catggcggta aaaccggcag taccgataat
     6241 cattgcttta cgcgcgtcca gcccttgcgg catggcaacc agccagtcac ctttcactcg
     6301 cgcctgctcc gccagcccac cccagtggtt ttcaccaacg ccccagccag tgagtaacat
     6361 ctcctgaccg gcatgaaaac gcggatcttc gctggtgcgt acagttccgg caaaatcgat
     6421 cccaggaatc atcggaaaat tacggatgat ttttccctta ccggtaatcg ccagcgcatc
     6481 tttatagttc aggctcgacc agtgaacatc aaccgtgaca tcgccctccg gcaggcgact
     6541 ttcgtcaagt gtctgtaccg atgcgagagt tttgccgtcc tgctgttcta aaagtaacgc
     6601 ctgcataagt ggtcctcatg tgcatgatgg attggaaaat aatttctgaa gactatactc
     6661 gctaatggaa aagcaaagcc gatgaagcgc aataaattgc gagataaatc tgatttgcta
     6721 gtatgcccgc ttcctcacta tcggagttaa cacaaggatg agattaacga cgaaattttc
     6781 ggcctttgtt acgctgctca ccgggttaac aatttttgtg actttgctgg gctgttcgct
     6841 aagtttctac aacgcaattc agtataagtt tagtcatcgc gttcaggcgg tggcgacggc
     6901 gattgatacc caccttgtgt cgaatgactt cagcacatta aggccacaaa ttaccgaatt
     6961 aatgatgtcg gcagatatcg ttcgtgtaga cctgctccat ggtgataaac aggtttatac
     7021 cctggccaga aatggtagtt atcgtccggt tggctccagc gatctgtttc gcgaactgag
     7081 tgttccgttg ataaagcatc cggggatgtc gctgcgtctg gtttatcagg atccgatggg
     7141 caactatttc cattctttaa tgactaccgc gccgctcacg ggggcgattg gctttatcat
     7201 tgttatgctc ttcctggcgg tacgctggtt acaacggcaa cttgccgggc aagaattgct
     7261 ggaaacccgg gctactcgta tcttaaacgg tgagcgtggc tctaatgtgt tgggaaccat
     7321 ctatgaatgg ccgcccagaa ccagcagtgc gctagatacg ctgcttcgtg aaattcagaa
     7381 cgcacgcgaa caacacagcc gtcttgatac gctgatccgc tcttatgccg cccaggacgt
     7441 gaaaaccggc ctcaataacc gactcttttt cgataatcag ttagcaacgt tactggaaga
     7501 tcaggagaaa gtaggtaccc acgggatcgt gatgatgatt cgtctgccgg atttcaatat
     7561 gttgagcgat acttgggggc acagccaggt tgaagaacag ttcttcactc tgacgaatct
     7621 gctgtcgaca tttatgatgc gctaccctgg cgcactgctg gcgcgttacc accgcagtga
     7681 ttttgctgcg ctgttaccgc accggacgtt aaaagaggca gagagcatcg ccggtcagtt
     7741 aatcaaagcc gtcgatacct tgccgaacaa taaaatgctc gatcgcgacg atatgatcca
     7801 cattggtatc tgcgcctggc gtagtggtca ggataccgag caggtaatgg aacatgcaga
     7861 gtctgccacg cgtaatgcgg gattgcaggg cggcaatagc tgggctattt acgatgactc
     7921 gttgcctgaa aaaggacgcg gtaatgttcg ctggcgtacg cttatcgagc aaatgctcag
     7981 tcgcggcggc ccgcgccttt atcaaaaacc ggcggttact cgcgaaggtc aggttcatca
     8041 tcgcgaactc atgtgccgca tcttcgatgg taatgaagag gttagctcgg cggagtatat
     8101 gccgatggtc ttgcagtttg gcttatcgga agagtatgac cgtctgcaaa tcagccgtct
     8161 tattccacta ttgcgttact ggccagagga aaatctggcg attcaggtta ccgttgagtc
     8221 gctgattcgc ccgcgttttc agcgttggct gcgcgatacg ttaatgcaat gtgaaaaatc
     8281 acaacgaaaa cgcataatta ttgaacttgc agaggccgat gtaggtcaac atatcagtcg
     8341 tttacaacct gttattcgtt tagtaaatgc tttaggggta cgggtagccg tcaaccaggc
     8401 tggtttgacg ctggtaagca ccagttggat caaagaactt aatgttgagt tactcaagct
     8461 ccatccgggg ctggtcagaa acattgagaa gcgaacggag aaccagctgc tggttcaaag
     8521 cctggtggaa gcctgctccg ggaccagcac ccaggtttac gccaccggtg tgcgttcgcg
     8581 aagcgagtgg cagaccctga ttcagcgcgg tgttacaggc gggcaagggg attttttcgc
     8641 gtcctcacag ccacttgata ctaacgtgaa aaaatattca caaagatact cggtttaacc
     8701 tgccgtttaa tccgttttca cgtagaataa cgcgcgctgc gtctcatggg agtgtgcttg
     8761 tctgctcgcc agattgttgc agcacatatg cagatgaatg accttacgcg gttgcaaaca
     8821 ggcgaggaat gctgctgatg cattaagcct ttctggactc aggcagagat ttgtaacaaa
     8881 ggaaacgaac tgcactaatt ttcaccgtag cagatgattt ttgcgccttg tcgctgctgc
     8941 gtgtggttgg taaagtaagc ggattttctt ttccgcccca gctttcagga ttatccctta
     9001 gtatgttgaa aaaatttcgt ggcatgtttt ccaatgactt gtccattgac ctgggtactg
     9061 cgaataccct catttatgta aaaggacaag gcatcgtatt gaatgagcct tccgtggtgg
     9121 ccattcgtca ggatcgtgcc ggttcaccga aaagcgtagc tgcagtaggt catgacgcga
     9181 agcagatgct gggccgtacg ccgggcaata ttgctgccat tcgcccaatg aaagacggcg
     9241 ttatcgccga cttcttcgtg actgaaaaaa tgctccagca cttcatcaaa caagtgcaca
     9301 gcaacagctt tatgcgtcca agcccgcgcg ttctggtttg tgtaccggtt ggcgcgaccc
     9361 aggttgaacg ccgcgcaatt cgtgaatccg cgcagggcgc tggtgcccgt gaagtcttcc
     9421 tgattgaaga accgatggct gccgcaattg gtgctggcct gccggtttct gaagcgaccg
     9481 gttctatggt ggttgatatc ggtggtggta ccactgaagt tgctgttatc tccttgaacg
     9541 gcgtggttta ctcctcttcc gtgcgcattg gtggtgatcg tttcgacgaa gctatcatca
     9601 actatgtgcg tcgtaattac ggttctctga tcggtgaagc caccgcagaa cgtatcaagc
     9661 acgaaatcgg ttcggcttac ccgggcgatg aagtccgtga aatcgaagtt cgtggccgta
     9721 acctggcaga aggtgttcca cgcggtttta ccctgaactc caacgaaatc ctcgaagcac
     9781 tgcaggaacc gctgaccggt attgtgagcg cggtaatggt tgcactggaa cagtgcccgc
     9841 cggaactggc ttccgacatc tccgagcgcg gcatggtgct caccggtggt ggcgcactgc
     9901 tgcgtaacct tgaccgtttg ttaatggaag aaaccggcat tccagtcgtt gttgctgaag
     9961 acccgctgac ctgtgtggcg cgcggtggcg gcaaagcgct ggaaatgatc gacatgcacg
    10021 gcggcgacct gttcagcgaa gagtaatcgg atgcaggcag gggaaatgtc tgtttaccct
    10081 gcctggtctg atacgagaat acgcataact tatgaagcca atttttagcc gtggcccgtc
    10141 gctacagatt cgccttattc tggcggtgct ggtggcgctc ggcattatta ttgccgacag
    10201 tcgcctgggg acgttcagtc aaatccgtac ttatatggat accgccgtca gtcctttcta
    10261 ctttgtttcc aatgctcctc gtgaattgct ggatggcgta tcgcagacgc tggcctcgcg
    10321 tgaccaacta gaacttgaaa accgggcgtt acgtcaggaa ctgttgctga aaaacagtga
    10381 actgctgatg cttggacaat acaaacagga gaacgcgcgt ctgcgcgagc tgctgggttc
    10441 cccgctgcgt caggatgagc agaaaatggt gactcaggtt atctccacgg ttaacgatcc
    10501 ttatagcgat caagttgtta tcgataaagg tagcgttaat ggcgtttatg aaggccagcc
    10561 ggtcatcagc gacaaaggtg ttgttggtca ggtggtggcc gtcgctaaac tgaccagtcg
    10621 cgtgctgctg atttgtgatg cgacccacgc gctgccaatc caggtgctgc gcaacgatat
    10681 ccgcgtaatt gcagccggta acggttgtac ggatgatttg cagcttgagc atctgccagc
    10741 gaatacggat attcgtgttg gtgatgtgct ggtgacctcc ggtctgggcg gtcgtttccc
    10801 ggaaggctat ccggtcgcgg ttgtctcttc cgtaaaactc gatacccagc gcgcttatac
    10861 tgtgattcag gcgcgtccga ctgcaggact gcaacgtttg cgttatctgc tgctgctgtg
    10921 gggcgcggat cgtaacggcg ctaacccgat gacgccggaa gaggtgcatc gtgttgctaa
    10981 tgaacgtctg atgcagatga tgccgcaggt attgccttcg ccagacgcga tggggccaaa
    11041 gttacctgaa ccggcaacgg ggatcgctca gccgactccg cagcaaccga cgacaggaaa
    11101 tgcagctact gcgcctgctg cgcccacaca gcctgctgct aatcgctctc cacaaagggc
    11161 tacgccgccg caaagtggtg ctcaaccgcc tgcgcgtgcg ccgggagggc aatagtggcg
    11221 agctatcgta gccagggacg ttgggtaatc tggctctctt tcctcattgc gctgttgctg
    11281 caaatcatgc cctggccgga taacctgatt gttttccggc caaactgggt gttactcatc
    11341 ttgttgtatt ggatcctggc cttgcctcat cgcgtaaatg tgggcacagg ttttgtgatg
    11401 ggtgccatac tggatctgat cagcggctcg acgcttggcg tacgcgtatt ggcgatgagc
    11461 atcattgctt acctggtggc gctgaaatac cagcttttcc gcaacctcgc attatggcag
    11521 caggcgctgg tcgtcatgtt gctttcgctg gtggtggata ttattgtttt ctgggcagag
    11581 tttttagtga ttaacgtctc tttcagaccg gaagtgttct ggagtagtgt agtcaatggg
    11641 gtgctctggc cgtggatttt cttgctgatg cgtaaagtcc gtcagcagtt tgcagtgcaa
    11701 taaaggtttc tatgacttct ctgtatttag cttccggttc tccgcgtcgt caggagttac
    11761 ttgcgcaact tggcgtgacc tttgaacgta ttgttacggg cattgaggag cagcgtcagc
    11821 cgcaggagag cgcgcagcag tatgttgtgc gtctggcgcg cgagaaagca caggcgggtg
    11881 tcgcgcaaac ggcgcaggat ctcccggtgc tgggtgcgga tactatcgtt atcctgaatg
    11941 gagaagtgct ggagaaaccg cgcgacgcag agcatgcggc gcagatgttg cgcaaattat
    12001 cgggtcagac ccatcaggtg atgacggcag tggcgttggc cgacagccag cacattctcg
    12061 attgcctggt ggtcaccgat gtgactttca gaacgttaac agacgaagac atcgcgggct
    12121 atgtcgccag cggtgaaccg ttagataaag caggtgcata cggtattcag gggctgggtg
    12181 gctgttttgt caggaagata aatggcagct atcacgccgt agtcggctta ccgctggttg
    12241 aaacgtatga attattaagt aattttaacg cactgcgtga gaaaagggat aaacatgacg
    12301 gctgaattgt tagtaaacgt aacgccttcg gaaacgcgag tggcgtatat tgatggcggt
    12361 attctgcagg aaattcatat tgaacgtgag gcgcgacgcg gaatagtagg caatatctac
    12421 aagggtcgtg taagtcgtgt acttccgggt atgcaggcgg cttttgtaga tattgggctg
    12481 gataaagccg cgtttcttca tgcatccgat atcatgccgc acaccgaatg tgtggcgggt
    12541 gaagaacaaa agcaattcac ggtgcgcgac atctcggaac tggttcgtca ggggcaagat
    12601 ctgatggtgc aggtggtgaa agatccgctt ggcactaaag gtgcgcgcct gaccaccgat
    12661 atcacgctgc cttctcgcta tctggtgttt atgccagggg cttctcacgt tggggtttcc
    12721 caacgtattg aaagcgaatc agaacgtgaa cgcctgaaaa aagtggtcgc agagtattgc
    12781 gacgagcagg gcgggtttat catccgtacc gcagcggaag gggttggcga ggctgaactg
    12841 gcctccgatg ccgcttatct gaaacgcgtc tggaccaaag ttatggagcg taaaaaacgc
    12901 ccgcagaccc gttatcagct gtacggcgaa ctggcgctgg cgcagcgtgt tctgcgtgat
    12961 ttcgccgacg ccgaactgga ccgcattcgc gttgactcac gcctgactta cgaagcattg
    13021 ctggagttta cctcggagta cattcccgag atgaccagca agctggagca ttacaccgga
    13081 cgccagccga ttttcgatct ctttgatgtc gaaaacgaaa tccagcgagc gctggaacgc
    13141 aaagtagaac tgaaatccgg tggttatctg attatcgacc agaccgaagc gatgaccacc
    13201 gtggacatca ataccggagc gtttgtcggt catcgcaatc ttgacgacac cattttcaat
    13261 accaatattg aagcgacgca ggctatcgct cgccagttac ggttgcgcaa tctgggcggg
    13321 attatcatta ttgatttcat cgatatgaat aatgaagatc accgccgccg agtgctgcac
    13381 tcgctggagc aggcgttgag caaagaccgg gtgaaaacca gcgttaatgg tttttcggcg
    13441 ctggggctgg tggagatgac gcgtaaacgc acccgcgaaa gcattgagca cgtactgtgt
    13501 aacgaatgcc caacctgcca cggtcgcgga acggtgaaaa ccgtggaaac ggtatgctat
    13561 gaaatcatgc gcgagattgt tcgtgtccac catgcttacg actccgaccg tttcctggtc
    13621 tatgcttctc cggcagtagc tgaagccttg aaaggcgaag agtcacactc gctggcggaa
    13681 gtggaaattt tcgttggcaa acaggttaaa gtacaaattg aaccgctcta taaccaggag
    13741 cagtttgacg tcgtaatgat gtaaacagat gctgggccgc catccggcaa agggtttttg
    13801 agtcacattt ttagcagaca aggagtgacg ggtgaggcga ttgccgggga ttttactgct
    13861 tactggagcc gcgctcgttg tgatcgctgc cctgctggtt agcggcctgc gtattgcttt
    13921 accgcatctt gacgcctggc gtccggaaat cctcaacaaa atagaatccg cgactggcat
    13981 gccggtagaa gccagtcagc tctcagccag ctggcagaat tttggcccga cgcttgaagc
    14041 acacgacatc cgtgcagaac taaaagatgg cggcgaattt tcggttaaac gcgttactct
    14101 ggcgctggat gtctggcaga gcttgttaca tatgcgctgg cagtttcgcg acctcacttt
    14161 ctggcagctg cgctttcgca ccaacactcc tatcaccagc ggtggtggta atgatagcct
    14221 ggaagccagt cacatcagcg atctgtttct tcgtcaattt gaccatttcg atctccgcga
    14281 cagtgaagtc agttttctga cgccatccgg tcagcgcgcc gagctggcga tcccacaact
    14341 cacctggctg aacgatccac gtcgacaccg tgcggaaggc ctggtaagcc tctccagcct
    14401 taccggacag cacggcgtga tgcaggtgcg tatggatttg cgcgatgatg aggggttgtt
    14461 aagcaatggt cgcgtctggc tacaggcgga tgacatcgac ctgaagccgt ggctcggtaa
    14521 atggatgcag gacaatattg ctctggaaac ggcacagttc tcccttgaag gctggatgac
    14581 gatcgacaaa ggcgatgtaa ccggcggtga cgtctggctg aaacagggcg gtgccagctg
    14641 gttgggcgag aagcaaacgc atacgctgtc ggtggataat ctgaccgcgc atattacgcg
    14701 tgaaaatccg ggctggcagt tctctattcc cgatacacgg atcacgatgg acggcaaacc
    14761 ctggccgagc ggagcattga cgctggcctg gataccggaa caggacgttg gcggcaaaga
    14821 caataaacgc agtgacgaac tccggattcg cgccagtaat ctggagctgg caggcctgga
    14881 gggcgtacgc ccgctggccg cgaaactttc acctgcactg ggtgatgttt ggcgctccac
    14941 acaaccgagc ggcaagatta acactctggc gctggatatc ccgcttcagg cggcagacaa
    15001 gacccgtttt caggcatcgt ggagcgatct ggcctggaag caatggaaat tattaccggg
    15061 tgcggaacac ttctccggga cgctttccgg cagcgttgaa aatggtttgc ttaccgcgtc
    15121 gatgaagcag gcaaagatgc cttacgaaac ggtattccgt gcgccactgg aaatcgccga
    15181 cggccaggca actataagct ggctgaacaa tgacaaaggt ttccagctgg atgggcgtaa
    15241 tattgacgtt aaagccaaag ccgtccatgc gcgcggcggt tttcgttacc tgcaacctgc
    15301 taacgatgaa ccctggctgg gtattctggc tggcatcagt accgatgatg gttcacaagc
    15361 ctggcgctat ttcccggaaa acttgatggg taaagacctg gttgattatt taagtggtgc
    15421 gattcagggc ggtgaagcgg ataacgcgac gctggtttat ggtggcaatc cgcaactctt
    15481 cccctataaa cacaacgaag gtcagtttga agtgctggtg ccgctgcgca acgcgaagtt
    15541 tgccttccag ccggactggc ctgcattaac taaccttgat attgaactgg actttattaa
    15601 cgacggttta tggatgaaaa ccgatggcgt taatctgggc ggcgtgcgcg cgagtaatct
    15661 caccgcagtg atccctgact actcaaaaga aaaactgctg attgacgctg acattaaagg
    15721 tccgggtaaa gccgtaggcc cttactttga tgagacaccg ctgaaagatt ccctgggtgc
    15781 gaccctgcaa gaactccagc tcgacggcga tgtgaatgct cgcttacatc ttgatatccc
    15841 gctgaacggc gagctggtaa ccgcgaaagg tgaagtgacg ctgcgtaata acagtctgtt
    15901 tatcaaacca ctcgacagca ccctgaaaaa tttgagcggt aaattcagct ttatcaatgg
    15961 cgatctgcaa agtgaaccac tgacagcaag ctggtttaat cagccgttga acgtggattt
    16021 ttccaccaaa gaaggggcaa aagcctacca ggttgcggtg aatctcaacg gtaactggca
    16081 accggcgaaa accggcgttt tgcctgaagc ggtgaacgaa gcattaagtg gcagcgtggc
    16141 gtgggatggt aaagtgggca ttgatctgcc ttatcatgct ggcgcgacct ataacgtaga
    16201 gctgaacggc gatctgaaga atgtgagtag tcacttacct tcaccgttag ccaaacctgc
    16261 gggtgaacca ctagcggtaa acgttaaggt tgatggcaat ctcaacagct ttgaattaac
    16321 cggacaggct ggtgcggata accatttcaa tagccgctgg ttgcttggtc aaaagctgac
    16381 gctcgatcgt gctatttggg cggcagacag taaaacgctc ccgccgttgc cggaacaaag
    16441 tggcgttgaa ctcaatatgc cgccgatgaa tggtgccgag tggctggccc tgttccagaa
    16501 aggcgctgcg gagagtgtcg gtggtgcagc gagtttccca caacacataa cgttacgtac
    16561 gcctatgttg tcactgggaa atcagcaatg gaataacctg agtattgttt cgcaaccgac
    16621 ggcaaatggc accctggttg aggcgcaagg gcgtgaaatc aacgccacgc tagcgatgcg
    16681 taataacgcg ccgtggctgg cgaatatcaa atatctttat tacaacccga gcgtggcgaa
    16741 aactcgtggt gattcaacgc cgtcatcacc tttcccgaca acggagcgca ttaacttccg
    16801 tggctggccg gacgcccaaa tacgatgcac agagtgctgg ttctgggggc aaaaattcgg
    16861 tcgcattgac agtgatatca ccatttctgg cgatacgtta acgctgacca atggactgat
    16921 tgatactggt ttctcgcggc ttactgccga tggtgaatgg gttaataatc cggggaatga
    16981 acgtacctcg ctgaaaggaa aactgcgcgg gcagaaaatt gatgccgccg cagaattttt
    17041 tggtgtcacg acgcccatac gccagtcgtc atttaatgtg gattacgatt tacactggcg
    17101 caaagcaccc tggcagccag atgaagcgac gttgaatggc atcattcata ctcaactggg
    17161 taaaggcgaa attaccgaaa tcaataccgg acatgccggg caattgctgc gcttattgag
    17221 cgtagatgcc ctgatgcgta agctgcgttt tgatttcaga gacacttttg gcgaagggtt
    17281 ttattttgac tccattcgca gcaccgcgtg gattaaagac ggcgttatgc acaccgacga
    17341 cacgctggtg gatggcctgg aggcggatat cgccatgaaa gggtcggtaa atctggtacg
    17401 tcgcgacctg aatatggaag cggttgtcgc accagagatt tctgcgacgg tgggcgtggc
    17461 tgcggctttt gcggttaacc ccattgttgg cgcggcagtg tttgccgcca gtaaagtgct
    17521 ggggccgctg tggagcaaag tctccatttt gcgctatcac atttcgggtc cgctggacga
    17581 tccgcaaatc aacgaagtgt tgcgccaacc gcgtaaagaa aaagcgcaat gatttgacga
    17641 gggcgcgtaa ttgccccaat ctcatatgat aatcgttgcc aaaggccaac gagccagaac
    17701 ataaccgtag gtcggatagg gcgttcacgc cgcatccggc agccgtaaaa aatcctctac
    17761 tgcagtaact aacgagtagc aaaaacgatg agtcttaacc tggtaagtga acaattgcta
    17821 gcggcgaacg gcctgaaaca tcaggacttg ttcgcgatcc tcggtcaact ggccgaacgt
    17881 cgccttgatt atggcgatct ctattttcag tcgagctatc acgaatcctg ggttttagaa
    17941 gaccgcatta ttaaagatgg ttcttacaac atcgatcagg gcgttggtgt gcgtgcaatc
    18001 agtggtgaaa aaaccggatt tgcttacgct gaccaaatca gcctgctggc gctggaacag
    18061 agtgcgcaag cggcgcgcac catcgtccgt gatagtggtg atggcaaagt acagacgctg
    18121 ggcgcggtag agcatagccc gttgtatacc tcggtagatc cgctgcaaag catgagccgt
    18181 gaagagaagc tggatatcct gcgtcgcgtc gataaggttg cccgcgaagc ggacaagcgc
    18241 gtacaagaag tgactgccag cctcagcggc gtttatgaat taattctggt tgcggccacc
    18301 gacggcacgc tggcggcgga tgttcgtccg ctggtgcgtc tttccgtgag cgttctggtc
    18361 gaagaagatg gcaaacgcga acgcggtgcc agtggcggcg gcggtcgttt tggttatgag
    18421 ttcttccttg ccgatcttga cggcgaagtc cgtgcggatg catgggcaaa agaagcagtg
    18481 cgtatggcgc tggtcaatct ttctgccgtt gctgcaccag cgggcaccat gccggtagta
    18541 cttggcgcag gttggccggg cgtgctgttg catgaagcgg taggtcacgg tctggaaggc
    18601 gacttcaacc gccgcggcac ttcagtattt agtgggcagg tcggggagct ggtggcttca
    18661 gaactgtgta ccgtggttga tgacggcacg atggtcgatc gccgtggttc ggtggcgatt
    18721 gatgacgaag gtacgccagg ccagtacaac gtgctgattg agaacggcat tctgaaaggc
    18781 tacatgcagg ataaactcaa cgcgcgtttg atggggatga cgccgactgg caacggtcgc
    18841 cgtgaatcct acgcccatct gcccatgccg cgtatgacca acacctatat gctgccgggt
    18901 aaatcgaccc cgcaggaaat tattgaatcc gttgagtacg gtatctatgc accgaacttt
    18961 ggtggcggtc aggtggatat tacatccggc aaattcgttt tctccacttc agaagcgtat
    19021 ctgattgaaa acggtaaagt aacgaagccg gtgaaaggcg cgacgttgat tggttccggt
    19081 atcgaaacca tgcagcagat ttcgatggtt ggcaacgacc tgaaactgga taacggcgtg
    19141 ggtgtctgcg gtaaagaagg gcaaagtttg ccggttggcg tgggccagcc aacgctgaaa
    19201 gttgataacc tgactgttgg cggtactgcg taataatttc attattttca gaaggataat
    19261 taatctttct acgtgcacga acggtcccct cgctcctctg gggttagggt gaggggaacc
    19321 cgttggcaca ggtttgtaga acgtaacagt acaatatgaa ttacttctct ttcccgcgcc
    19381 cgtgcatctc ctgaaacaat ttaccgacct caacaaaata atccgtcagc gagttgatca
    19441 cgacctgtac cttcagcggc agcttatctt tttcggtata taacgcataa accgggcgtg
    19501 gatctgactg gtaacgtggt agcaggatct ccagctcccc gcgattgatc tcgttgatca
    19561 cccacatcag cggcacgtag gcgatcccag cgcctgcagt cagccagcgc accagcgtca
    19621 tcggatcatt agtcacaaat ctcccctgcg ggatcaggcg agtcgagatc ccttccggtg
    19681 cgatcagttc aaattcattg tcgggccgca cgctgtattc aagccatgaa tggctactta
    19741 aatcggcggg tttttccggt atgccgtatt gtgtgagata gctttttgcg gcgcacacca
    19801 ccattggcat cgcgcccaga cggcgggaaa acaggctgga atcctgcaac gcgccgacgc
    19861 ggatcaccac atccagaccg tcggcaatca ggtcgggggc aggaattccg gtaaccagat
    19921 tgacgctcaa acctgggtat tctttcagca tttttgctgt cagcccggcg agaacatttt
    19981 gtgccatagt tgaagaacag ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat
    20041 acagttgctc atgaacatcc tgcacttcat gaagcatacg acggcagccc tggtagtaaa
    20101 ttctaccggc ttcggtcagg ccaatgctgc gtgtgctacg gtttaacagc tttacctgca
    20161 actcatcttc cagttttgac accgtctgac tgatggacga aacgctcatc tgtagctgtc
    20221 tggcggcggc ggtaaaagag ccaaattcaa ctactttggc aaacaccgac atgcgtttta
    20281 gtcgttccat tattcactct gacttaaaag tgatttagat cacataatat agataacagc
    20341 ataacagtta cgctaatata ttaaatatca atctacagca atgttgctct cgcccggctt
    20401 tccatgccct tctctgcggc gacagatgct gaaaataata acgcctgctc tctctttaca
    20461 accaaggtca acatgagtct gtttcccgtt atcgtggtgt ttgggctgtc cttcccaccg
    20521 atattttttg aattgctttt atcactggcg attttctggc tggtgcgccg ggtacttgtg
    20581 ccaacaggta tctacgactt tgtctggcat ccggcgttgt tcaacaccgc gctctattgc
    20641 tgcttgtttt atttgatatc gcgactgttc gtttgaggtt gaagtgaaaa cactaataag
    20701 aaaattctcc cgtacggcca tcacggtcgt attagtcatt ctggccttca tcgcaatttt
    20761 taatgcctgg gtctattaca ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga
    20821 cgtcgttgcg atcgcgccgg acgtttctgg actcattacc caggtgaatg ttcatgataa
    20881 ccagctggtg aaaaaaggac agatactgtt caccatcgac cagccgcgct atcaaaaggc
    20941 gcttgaggaa gcgcaagccg atgttgctta ttatcaggta ctggcacagg agaaacgcca
    21001 ggaggccgga cgtcgtaacc gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca
    21061 ggctaacaac gtactacaaa cggttctgca tcagttagcg aaagcgcagg cgacccgcga
    21121 tctggcaaaa ctggatcttg aacgcacggt gatccgcgcg ccagcagatg gctgggtgac
    21181 caacctcaac gtctataccg gtgagtttat tactcgagga tcaacggcgg ttgcgctggt
    21241 gaaacagaac tccttctatg tactggccta tatggaagaa actaagctgg aaggggtgcg
    21301 tccggggtat cgtgcagaga tcacgccgct tggcagtaac aaagtgctga aagggactgt
    21361 tgatagtgtt gccgcagggg tcaccaacgc cagcagcacg cgtgacgaca aagggatggc
    21421 gactatagac tctaaccttg aatgggtgcg tcttgcgcaa cgtgttccgg ttcgtattcg
    21481 tctcgacaac cagcaagaga acatctggcc tgcgggcacc actgctacag tggtggtcac
    21541 tggcaaacaa gatcgcgacg aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct
    21601 gcgtgagttt ggttaatcac gatgggtatt ttctccattg ctaaccaaca tattcgcttt
    21661 gcggtaaaac tggcgaccgc cattgtactg gcgctgtttg ttggctttca cttccagctg
    21721 gaaacgccac gctgggcggt actgacagcg gcgattgttg ccgccggtcc ggcctttgct
    21781 gcgggaggtg aaccgtattc tggcgctatt cgttatcgtg gctttttgcg catcatcggc
    21841 acatttattg gctgtattgc cggactggtg atcatcattg cgatgatccg cgcaccatta
    21901 ttgatgattc tggtgtgctg tatctgggcc ggtttttgta cctggatatc ctcgctggta
    21961 cgaatagaaa actcgtacgc gtgggggctg gccggttata ccgcgctgat cattgtgatc
    22021 accattcagc cggaaccatt gcttacgccg cagtttgccg tcgaacgttg tagcgagatc
    22081 gttatcggta ttgtgtgtgc gattatggcg gatttgctct tttctccgcg atcgatcaaa
    22141 caagaagtgg atcgagagct ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc
    22201 tgtatcaagc atggcgatgg tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc
    22261 accacggcgc tacaaggcat gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg
    22321 gccaatcgac gtttaaaagc gatcaatacg ctatcgctga cgctgattac ccaatcctgc
    22381 gaaacttatc ttattcagaa tacgcgcccg gaattgatca ctgatacttt ccgcgaattt
    22441 tttgacacgc cggtagaaac cgcgcaggac gtccacaagc agctcaaacg cctgcggaga
    22501 gttatcgcct ggaccgggga acgggaaacg cctgtcacca tttatagctg ggtcgcggcg
    22561 gcaacgcgtt atcagcttct caagcgcggc gttatcagta acacaaaaat caacgccacc
    22621 gaagaagaga tcctgcaagg cgaaccggaa gtcaaagtag agtcagccga acgtcatcat
    22681 gcgatggtta acttctggcg aaccacactt tcctgcattc tgggcacgct tttctggctg
    22741 tggacgggct ggacttccgg cagtggtgca atggtgatga ttgcggtagt gacgtcactg
    22801 gcaatgcgtt tgccgaatcc acgcatggtg gcgatcgact ttatctacgg gacgctggcc
    22861 gcgctgccgt tagggctgct ctactttttg gtgattatcc ctaataccca acagagcatg
    22921 ttgctgctgt gtattagcct ggcagtgctg ggattcttcc tcggaataga agtacagaaa
    22981 cggcgactgg gctcgatggg ggcgctggcc agcaccataa atattatcgt gctggataac
    23041 ccgatgactt tccatttcag tcagtttctc gacagcgcat tagggcaaat cgtcggctgt
    23101 gtgctcgcgt tcaccgttat tttgctggtg cgggataaat cgcgcgacag gactggacgt
    23161 gtactgctta atcagtttgt ttctgccgct gtttccgcga tgactaccaa tgtggcacgt
    23221 cgtaaagaga accacctccc ggcactttat cagcagctgt ttttgctgat gaataagttc
    23281 ccaggggatt tgccgaaatt tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg
    23341 cgtgatgcgc cgatcccggt taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc
    23401 acagcagacc atgtgatatc tgcccgtagc gatgataaac gtcgtcggta ctttgatcag
    23461 ttgctggaag aactggaaat ctaccaggaa aagctacgca tctggcaagc gccaccgcag
    23521 gtgacggaac cggttcatcg gctggcgggg atgctccata agtatcaaca tgcgttgacc
    23581 gatagttaag tcaaaaccga cgccaaaagc gtcggttttt tcatggctat acttagcgat
    23641 gaacggcaga actcgccgcg aaacgtgacg gtggcaacag atgaatattt atacctttga
    23701 ttttgatgag attgagagtc aggaggattt ttatcgcgac ttcagccaag cctttggtct
    23761 ggcgaaagat aaggtacgcg atctcgactc actatgggat gtgttaatga acgatgtcct
    23821 gccgctacca cttgagattg aatttgttca tctgggagag aaaacgcgtc gccgttttgg
    23881 cgcgttaata ttgctgtttg atgaggcaga ggaagagctg gaagggcatt tgcgttttaa
    23941 tgttcgtcat tagcgcaaaa aaaagccccc gaaccggggg caatatcgtc ggacaagacg
    24001 atgagggttt atttgtacag ttcagccgta gcgtgccagg tgtcaccgct acgagcttca
    24061 gtaatctggt aggccgttgc gcctttctct tccgcttttt tgttcagcat ttcacgtata
    24121 tccattggcg aagacgccac accacttacg gatacggtcc cgattgcttc acggttttgt
    24181 gcttgtgcag catcaatgga gtcggcagcg aatgcaccga aagagagaac agaaagtacg
    24241 cttaatgcag caacagtggt tttgattttc atgattttta cctcgacata atctttttgc
    24301 tgggtctttg tttcgtgacc cttatcacaa aatcaagta
//
LOCUS       DAFNBJ010000069        23459 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000069 DAFNBJ010000000
VERSION     DAFNBJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.069"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..389
                     /locus_tag="L2X49_002562"
     CDS             <1..389
                     /locus_tag="L2X49_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBN4797634.1"
                     /translation="NKNTMSESVRTECEKSSDRHQETDKAFEEIFWCAGMRKAGKKNA
                     ASAFRTQFREWRKTTRGTASEFATMLAEDIACRNGKQFGFDRLLPSSYLNGQRWNDEK
                     PETIQPQSKPSSAITVSKTGYVFFDR"
     gene            497..2377
                     /locus_tag="L2X49_002563"
     CDS             497..2377
                     /locus_tag="L2X49_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBN4797635.1"
                     /translation="MTPSELSDLLWAQVDRVAPHLLPNGKREGHEWVAGNVNGDKGNS
                     LKVNLSGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDAKR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVNGHGKTEVVGHMALEAMRQGVKTCIA
                     SLELKPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFD
                     YARRRYGIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDS
                     EEKPTGKMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPAS
                     VLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVT
                     EY"
     gene            2454..2780
                     /locus_tag="L2X49_002564"
     CDS             2454..2780
                     /locus_tag="L2X49_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797636.1"
                     /translation="MADWQIPIIILAGASLVAGFILLKKHKDRDQKVEVLYGYPANST
                     TWLTIYHYRKSGRWVFEWDDLFAEKRPKSWGDISECMMFEERKSGATREEFNEAWARL
                     SERGYL"
     gene            2777..2977
                     /locus_tag="L2X49_002565"
     CDS             2777..2977
                     /locus_tag="L2X49_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797637.1"
                     /translation="MSKYEKLDQNILSMLSERPTPVFDIWLKWRSNGMYIETIDRRMQ
                     YLRKKGLVANVRGKGWVKINLS"
     gene            2989..3162
                     /locus_tag="L2X49_002566"
     CDS             2989..3162
                     /locus_tag="L2X49_002566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797638.1"
                     /translation="MDESRKQFLEWFGEEFESINNSEELHVQAIKMIAWQAWLESRAV
                     IAKAINQKGERND"
     gene            3155..3301
                     /locus_tag="L2X49_002567"
     CDS             3155..3301
                     /locus_tag="L2X49_002567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797639.1"
                     /translation="MTEDFKSWFDKNFPPHLWPVEEERQEVMTYTWLAWRDAIRAAGV
                     KVKE"
     gene            3304..3714
                     /locus_tag="L2X49_002568"
     CDS             3304..3714
                     /locus_tag="L2X49_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HBN4797640.1"
                     /translation="MKQTIFLRGKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIASQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLRGLEGEFVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            3711..3887
                     /locus_tag="L2X49_002569"
     CDS             3711..3887
                     /locus_tag="L2X49_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HBN4797641.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIQKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            3890..4258
                     /locus_tag="L2X49_002570"
     CDS             3890..4258
                     /locus_tag="L2X49_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2591 family protein"
                     /protein_id="HBN4797642.1"
                     /translation="MDYSQLSDFEINRMVGDIIFKGLWACKPETSGNNTNKWYYGNAD
                     TTFEPLNPLPDYCNDPSASWPVIAKHHISICAYERNNPGMKNEYWWEADRFCEFITID
                     NNPLRAAMIVFLMMQDANNA"
     gene            4251..4436
                     /locus_tag="L2X49_002571"
     CDS             4251..4436
                     /locus_tag="L2X49_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HBN4797643.1"
                     /translation="MLSPSQSIQYQKESVERALTCANCGQKLHVLEVHVCERCIYECL
                     NMVEHNEKYKQHRRIKK"
     gene            4796..4966
                     /locus_tag="L2X49_002572"
     CDS             4796..4966
                     /locus_tag="L2X49_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ninF"
                     /protein_id="HBN4797644.1"
                     /translation="MGAPNILEYLLASLTLLQKGRGQKKNWVFMKTMADHNCAELMSD
                     PNSSMYEEEDDE"
     gene            4959..5681
                     /locus_tag="L2X49_002573"
     CDS             4959..5681
                     /locus_tag="L2X49_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBN4797645.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEETAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            5681..5971
                     /locus_tag="L2X49_002574"
     CDS             5681..5971
                     /locus_tag="L2X49_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBN4797646.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMVGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            5968..6330
                     /locus_tag="L2X49_002575"
     CDS             5968..6330
                     /locus_tag="L2X49_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBN4797647.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            6327..6515
                     /locus_tag="L2X49_002576"
     CDS             6327..6515
                     /locus_tag="L2X49_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HBN4797648.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HIIVNGVLMVKQGKRGRP"
     gene            6512..7135
                     /locus_tag="L2X49_002577"
     CDS             6512..7135
                     /locus_tag="L2X49_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBN4797649.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADSILNTVTR"
     gene            7571..7774
                     /locus_tag="L2X49_002578"
     CDS             7571..7774
                     /locus_tag="L2X49_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBN4797650.1"
                     /translation="MYRMDKIREWFSYSFGGLTAMGGILSLNDWAVIIGILCTVGTFG
                     INWYYKRKEREDRLNGNVTGTTK"
     gene            7752..8249
                     /gene="rrrD"
                     /locus_tag="L2X49_002579"
     CDS             7752..8249
                     /gene="rrrD"
                     /locus_tag="L2X49_002579"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBN4797651.1"
                     /translation="MSPALRNSVIAAISGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIVLGKTYTEEECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            8246..8713
                     /locus_tag="L2X49_002580"
     CDS             8246..8713
                     /locus_tag="L2X49_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBN4797652.1"
                     /translation="MNRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAENDALRRNLDNGGRVLVKGKCPVPS
                     SAETSGASGMGNDATVELSPVAGRNVLGVRDGIIRDQTALRTLQEYIRTQCLR"
     gene            8701..8853
                     /locus_tag="L2X49_002581"
     CDS             8701..8853
                     /locus_tag="L2X49_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797653.1"
                     /translation="MPSMIAIILLIILHIWLCRQGDDHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            9018..9260
                     /locus_tag="L2X49_002582"
     CDS             9018..9260
                     /locus_tag="L2X49_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HBN4797654.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            9263..9703
                     /locus_tag="L2X49_002583"
     CDS             9263..9703
                     /locus_tag="L2X49_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBN4797655.1"
                     /translation="MARPTKYQEAYAEQARKLCLLGYTDAELADFFEVSESTINKWKL
                     DYPKFSESIKKGKAVADAEVSDRLYQRAMGFVAPDIDIRVIENRIVETPLEKYYPPDT
                     TAAIFWLKNRQKDKWRDKVDHELTGKDGGAIQIETSPMSTLFGK"
     gene            9700..11112
                     /locus_tag="L2X49_002584"
     CDS             9700..11112
                     /locus_tag="L2X49_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBN4797656.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSSEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEVIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGISSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            11115..13241
                     /locus_tag="L2X49_002585"
     CDS             11115..13241
                     /locus_tag="L2X49_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBN4797657.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQKEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDPMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYEAEYGKKPPTSLDVTSMTSWEYN
                     WFGADVIYIAKYYEVRKESVDVISYRHPITGEIATYDSDQVEDIEDELAIAGFHEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            13255..14139
                     /locus_tag="L2X49_002586"
     CDS             13255..14139
                     /locus_tag="L2X49_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HBN4797658.1"
                     /translation="MENELIIDGQVIDLSETQENAEETIIQTESQPENESQDDNGKEV
                     ATEPEKTEETPEDYALRIGDEEIQLNADDDDHIDGQPAPQWVKDLRKGFKETQKENRE
                     LRRQLEEALAKPAEHQQPQPDAIPPKPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQ
                     QKLRQQQEYQQRFQQRVEAHKQRAAKLPVKDYQEMEAIVLSELPPIQQEIIIHCADEG
                     SELLAYGLGKSQQLRQRVAAETDPIRAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGG
                     ADAKQDEFNKLCPGAKIE"
     gene            14151..15422
                     /locus_tag="L2X49_002587"
     CDS             14151..15422
                     /locus_tag="L2X49_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head protein"
                     /protein_id="HBN4797659.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISGKNKNNLISGKATGRVGNYITVPVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATASVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADANSDSGGDVTVTLSGVPIYDTTNPQYNSVSRQVE
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            15465..15650
                     /locus_tag="L2X49_002588"
     CDS             15465..15650
                     /locus_tag="L2X49_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797660.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFERLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            15625..16056
                     /locus_tag="L2X49_002589"
     CDS             15625..16056
                     /locus_tag="L2X49_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein p27"
                     /protein_id="HBN4797661.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDDQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSD"
     gene            16116..17534
                     /locus_tag="L2X49_002590"
     CDS             16116..17534
                     /locus_tag="L2X49_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HBN4797662.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGKLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANAPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARVF
                     DFEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            17534..18235
                     /locus_tag="L2X49_002591"
     CDS             17534..18235
                     /locus_tag="L2X49_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBN4797663.1"
                     /translation="MADSNLNTPVIVQATRLDTSILPRNIFSQSYLLYVINQGADVGA
                     IAGKANQAGQGAYDAQVKNDEQDVELADHDARITANTKAINLLEVRLTTAEGKIVVLR
                     SDVDYLLDEVIDIQAHLVTVDQRLDGVESDVSDIKSDYVSKTVTESQSLASPLDVKTS
                     YSVDGIQVVGARNTGWTAATGIPLLGSFNANQSYTVGTTYTQSEVAALATGLQQARQR
                     ILALETALRLHGLID"
     gene            18235..18690
                     /locus_tag="L2X49_002592"
     CDS             18235..18690
                     /locus_tag="L2X49_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HBN4797664.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VYGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQIYCAMIGLNRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            18693..19388
                     /locus_tag="L2X49_002593"
     CDS             18693..19388
                     /locus_tag="L2X49_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBN4797665.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSDKSAKYAAEAQKYAADL
                     QNQQFNTIMNNLKPFTPLAEKYVGSLENLSSLEGQGQALNQYYNSQQYKDLAGQARYQ
                     SLAAAEATGGLGSTSTSNQLATIAPTLGQQWLSGQMNNYQNLANIGLGALQGQANAGQ
                     TYANNMSQISQQSAALAAANANRPSGLQSAIGGAASGAIAGAQLGSIVPGIGTGIGAA
                     VGGGLGLLGSLLY"
     gene            19399..20748
                     /locus_tag="L2X49_002594"
     CDS             19399..20748
                     /locus_tag="L2X49_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBN4797666.1"
                     /translation="MATWQQGINSGGFLAGIGTQNENAPKASDINATLGLIRENNELA
                     RSGANNVGLTALRGLAGVADIYNQEQQQKAISAFNKVHADAWASGDPSGLFKFAQENP
                     AFVAQAQQAFSGLNEQQRNDMGDLAMRANVALSQGPEAYSKFITDNKDRLNRVGANAD
                     WMIQTGIQNPEQLSHMLTTMSLGALGPEKAFAVQDKMVGRQQEQQRINETIRNNDMTN
                     ARAIRGQDLSYKAQMARLNHDKYVFKQSQAALERAGQLQDMDVLSLNSQIAATGIDPL
                     TGKAATSARMSQAKRWLDGNNNYNNALITGERGIEKIDSLLGKKELEGIGRFEGRNID
                     GFTSAEGLANRNAIEELKSGAFVQNVQAMRGMGSLSNAEGQKLENLIAKLDITQPEEV
                     VRKQLSEIRSQYSVFQKVAAREAESMGYSSSGYDTYVSERKSGSDSNKSGFSSLWGD"
     gene            20748..22613
                     /locus_tag="L2X49_002595"
     CDS             20748..22613
                     /locus_tag="L2X49_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBN4797667.1"
                     /translation="MAKAWKDVIASPQYQALTEEQKAQAQAQYFDEVVAPKAGDKWAE
                     ARDQFYAAYPPPQQQKEEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGA
                     SLINAISQGLGGPKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAE
                     AANQKGDFAQNATKNAGVNLAAQGVLSAAAKGIGRGITAVRGEISPADQQLLKRAAAA
                     DVPVMTSDVVPPKTKLGNQLQGYSEGVIAGTGPMRAAQQDARTKLVNRFTEKYGDYDP
                     SVVVDSLKSGVAREKSLAKSKLNNLSGRMVGKPVDTSGAIRAIDGAVNELGKLKGVSD
                     TQTISALNDYKNAIQEITHGDDAFELLDKLRTQFRIDVKGDRTVLPSMSQTMVDRVYN
                     SLTNSLSKSIAKGLSPKDASAWRAGKADYAKMATHATQTRLKNVLNKGDLTPEAVNTI
                     VYGQYGSDIARLYGKLDQKGKDMLRAAYISKIADKVGDSPQKMMTELGKLQKQANGQV
                     FKTVFGGKNGKEIEGMLSILDATKRASEANVVTKTGMTLAPLVRVIGNLKTGGALLAG
                     ETGIGLMSRVYESPMARNALLRLANTKAGTPAYERALNNAANAIRPILANDATDR"
     gene            complement(22635..23306)
                     /locus_tag="L2X49_002596"
     CDS             complement(22635..23306)
                     /locus_tag="L2X49_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797668.1"
                     /translation="MFCSKLRSGYSMKKIDIYSDTSAYVIGSLGFLIFFVWQYQSLSP
                     GWRFLGMSLISLGAGIATQVLMYLFNGWLSKRVEKKRATSICRILAIPEDSTDQDDIA
                     KCWRYMIARYSNELLANRLSDLIGIVVTSVGTIISIGISIWYVGMIVYFVWNRDFNEP
                     SLLFIPLFFRVLAFICELLLSFFCNVLFNRYPGEARKFNKNYDELRRTDPFLSSKEFR
                     DSIRN"
BASE COUNT         6841 a         5165 c         5975 g         5478 t
ORIGIN      
        1 ataataaaaa cactatgtcc gaaagtgttc ggacggagtg tgaaaaatca tctgaccgtc
       61 accaagaaac cgacaaggca ttcgaggaaa tattctggtg tgcaggcatg cggaaagccg
      121 ggaagaaaaa cgcagcttcg gcattcagaa cacagttcag ggaatggcgt aaaactacca
      181 ggggtacggc aagcgagttt gccacgatgc tggcagaaga catcgcatgc aggaatggta
      241 agcagttcgg attcgacagg ttgttaccat cgagctacct gaacggtcag cgctggaacg
      301 acgagaagcc agaaactatt caaccacaat ccaaaccatc atccgcaatc accgtatcga
      361 aaactggcta cgtgtttttc gacaggtgaa ccatgaaatc aaaaatcaaa tcgctactgg
      421 tcgctggtta taaccacggc tggttaagta tttcgtttgt cgatttctgg tttaaaaatc
      481 tcaatctgag ggaatcatga ctccaagtga actcagtgac ctgctttggg cgcaggttga
      541 cagggtggct ccgcacctgt tgccaaacgg caagagagag gggcatgagt gggttgccgg
      601 taacgtcaac ggtgacaagg gaaacagcct taaggtcaac cttagcggca agaaaaaatg
      661 ggctgatttc gctgagggag acggcggtga catgcttgat ttgtggatgg catgtcgtgg
      721 aattaacctg catcaggcta tgcaggaagc gaaagccttt ctcggaatca aggatgacga
      781 tcaccatttc gatgccaaac gtgagaagaa attctccaga cctgaccgca agaaaatcgc
      841 ccgctacgtt accagaacag aatcccatct tgagtacctg caatcgcgtg gcatatcgcc
      901 agaagtcgta aagcgctacg aggttgtcag cggcaaggtg tggaatggag aacgagaact
      961 tgatgcactg gtgcttccgt acaaacgcga tggtgagttg ttgcaggtca agcgaatcag
     1021 cactgagcga ccggacggga agaaagtcat tatggcagaa ggtgattgcg aaccttgtct
     1081 gtttggatgg caggctctgg acgctggcgt gagggcggtt gtactttgcg aaggcgaaat
     1141 tgattgtatg agctatgcgc aatacggcat ctcggcgtta tccgtgccgt ttggtggcgg
     1201 gaaaggcgct aagcaacagt ggattgagtt tgagtatcac aacctcgaca ggtttgagga
     1261 aatattcatc tcgatggacg ttgatgatgt tggtcgtgaa gccgcaaggg aaatcgcaag
     1321 ccgactcggt gaacatcgtt gccgtcttgt tactctgccg tacaaagaca tcaacgaatg
     1381 cctgatgaac ggtgttaccg aggatgaaat ctggcagtac atcggcacgg catcctactt
     1441 cgatcctgaa gaactctaca gcgcgcgaga gttttaccag gacactatca acgctttcta
     1501 cggcaagcag cagtatctgt ttaatccacc gtgggaatct ctggcagata aattccagtt
     1561 ccgtgaggcc gagttgacgc tggtcaatgg tgtgaacggt cacggaaaaa cggaggttgt
     1621 cgggcatatg gcacttgagg caatgcgtca gggtgtgaag acgtgcatcg cgtcacttga
     1681 gctgaagcct ggtattctcc ttaagcgcct tacccgtcag gcgacgtgct gcaagatgcc
     1741 gccagtgctg gaaattgact ctgcatttaa attttatgac gagagacttt gggtgtttgg
     1801 tctgaccgga acggcgaaag ccgacaggct gatcgaaata ttcgactacg ctcgccgccg
     1861 atacgggatc cagttattca tcatcgacag cctgatgaaa tgcggcatag gtgacgatga
     1921 ctataacggg cagaaagcat ttgttgactc gatttgtgac ttcaaaaaca aaacaaactc
     1981 ccacgtcatt ctcgttactc actccagaaa aggagatagc gaagaaaaac caaccgggaa
     2041 aatggacgta aaaggctctg gagcgataac agacctgaca gacaaccttt ttatcatctg
     2101 gcgtaacaag gctcgcgaga gagcgttaca gagagttcag agtggtgaaa agatgtcaga
     2161 gaaggacgaa cagctactgg catctccggc atctgttttg atgcttgaaa agcaacgtaa
     2221 cggcgaaggt tgggaaggtg gtgtcccgtt gttccttgac gagcaatcgc accagttcct
     2281 gcaacttgaa tcaggatcgc catatagcta catcgccaat atgccgaaat cggaatatga
     2341 cgaggcgtgg cgacaggaaa acgtgacgga gtattaaatg accatctaca tcactgagct
     2401 aataacaggc ctgctggtaa tcgcaggcct ttttatttgg gggagaggga agtgtggctg
     2461 actggcaaat tccaatcatc attcttgccg gagcttcgct ggttgctggc tttatcctgc
     2521 tgaaaaagca taaagaccgt gatcaaaaag tcgaagttct ctatgggtat ccagcgaaca
     2581 gcacaacatg gctgaccatt taccactacc gaaaatcagg ccgctgggta ttcgaatggg
     2641 atgatctgtt tgctgaaaag cgaccaaagt catggggaga catcagcgaa tgcatgatgt
     2701 ttgaagaaag aaaatccggc gcaacccgag aagagtttaa cgaagcgtgg gcgcgattaa
     2761 gtgagagagg gtatttgtga gcaagtacga aaaattagat caaaacattc tttcaatgct
     2821 gagtgaaaga ccaacacctg tttttgatat ctggcttaaa tggcggagca atggaatgta
     2881 tatcgaaacc atcgatcgcc gtatgcaata cctgagaaag aaagggcttg ttgcaaatgt
     2941 gcgtgggaag ggttgggtga aaattaacct atcataacgg ggattgatat ggacgaatca
     3001 agaaagcagt ttttggagtg gtttggtgaa gagttcgagt ctattaacaa cagcgaagaa
     3061 cttcacgttc aggccatcaa gatgattgcc tggcaggcat ggctagagtc aagagcggta
     3121 attgcgaaag ctatcaatca aaaaggtgag cgaaatgact gaagatttca aatcatggtt
     3181 tgataaaaac ttccctccgc atttatggcc ggttgaggaa gaacgtcagg aagtaatgac
     3241 ctacacctgg ttagcatggc gtgatgccat ccgcgccgct ggagtcaaag tgaaggagta
     3301 acgatgaagc aaacaatctt cctccgtggt aaacaacaac agcaagccgc aatcaacgcc
     3361 atcctcgcaa caccactcga taaagacaag ccagttacca tccgcattac tgactacaag
     3421 cgcaaccttg accagaacgc aaaatttcac gcgatgctgg cggatatcgc aagtcaggtt
     3481 caatggtgcg gcaaatggtt aaaaccagaa cagtggaagg ttttgttgat tagcggtcat
     3541 gcagtggcaa caaaacagga agctgatgtt ttgcgcggcc ttgaaggcga attcgtcaac
     3601 attcgcgaaa gcagcgcgca gatgagtgtg aagcgtatgg caagtctgat cgagtacaca
     3661 acagcctggg ctattggtca gggtgtcaga tttaccgaca ggaggtacga atgagacgac
     3721 agcgacgaag tatcaccgac ataatctgcg aaaactgcaa ataccttcca acgaaacgct
     3781 ccagaaataa acgcaagcca atccaaaaag aatctgacgt aaaaaccttc aactacacgg
     3841 ctcacctgtg ggatatccgg tggctaagac atcgtgcgag gaaatgacaa tggattattc
     3901 acagttaagt gattttgaaa ttaacagaat ggtaggagac ataattttta aaggcctttg
     3961 ggcatgtaaa ccggaaacgt cagggaataa caccaacaaa tggtattacg gaaatgctga
     4021 tacaactttt gagccattaa accctttacc tgactactgc aatgatccga gcgcctcatg
     4081 gcctgtaatc gcaaaacatc atatcagcat atgtgcatac gaaagaaata atcctggaat
     4141 gaagaatgaa tattggtggg aggcagatag attttgtgaa tttattacca tagacaacaa
     4201 cccactccgc gccgccatga ttgtatttct catgatgcag gacgccaata atgcttagcc
     4261 catcccaatc cattcaatac cagaaagaaa gcgtcgagcg ggctttaacg tgcgctaact
     4321 gcggtcagaa gctgcatgtg ctggaagttc acgtatgtga gcgttgtata tatgaatgct
     4381 taaatatggt ggaacataat gagaaatata agcaacatag acgaattaag aaataaatta
     4441 tcatatgatg atgttttagg tgtacttaga tggaaagtgt ctccttctaa taatgttaag
     4501 gttggcagtg ttgctggtta cattaggagt gatggttata ggatgttaac tatcgatggt
     4561 gtaattactt atgctcacca cgttatatgg atgataaata atggtgagat tccattaggg
     4621 tataaaattg atcatataaa tggagtaagg tcagataata ggctatctaa tcttcgattg
     4681 gttacacacc aacagaatgc acagaaccaa aagagaaaaa taaccaattc gtcaggagtg
     4741 actggagtgt atttcaataa agagagatgc aagtggattg cgaatatatg cgtagatggg
     4801 cgcaccaaat atcttggaat atttgctagc atcattgacg ctattgcaga aaggaagagg
     4861 gcagaaaaag aactgggttt tcatgaaaac catggcagac cataactgcg cagaactgat
     4921 gagcgatccg aatagctcaa tgtacgagga agaagacgat gaatgagtta ataaatggca
     4981 atgccatcaa aatgacaagc attgaaatcg ctgagttggt gggtaagcgt catgacaatg
     5041 tgaaacgtac catcgaaacg ctggctaaaa atggtgttat caggcttcct caaattgagg
     5101 tttccgaaag aatcaataac ttagggttca atgttcagta cgagcattac gtcttcgagg
     5161 gcgaacaagg taagcgagac agtattgtcg ttgtagccca gttgtcgccg gaattcaccg
     5221 ctcgtcttgt tgaccgttgg cgagagcttg aagaaactgc ggttaatatc cccaaaacgc
     5281 taccagaagc gttgcgcctt gctgctgatc ttgctgagca gaaaatgcaa ctggaaaacc
     5341 agctcgcaat tgccgcacct aaagttgagt ttgccgatcg agttggcgag gccagcggaa
     5401 ttttgattgg aaactttgca aaggttgttg gaattggtcc aaacaaactg tttgcgtgga
     5461 tgcgcgatca caaaatcctt attgcttcag gttcccggcg caatgtgcca atgcaggaat
     5521 atatggatcg cggctatttc acagtgaaag aaacagcggt caacacaaat cacggaatac
     5581 agatatcgtt caccacaaaa atcaccggac gtggtcaaca gtggctgacc agaaagctgc
     5641 tcgataacgg aatgctgaaa gtaacagggg aggctgctta atggctaacc tacgcaaaga
     5701 agcacgcggc agagaatgcc aggtacgtat ttacggcgta tgcaatggca atcctgaaac
     5761 tacagttctg gcacattacc ggatggttgg aatttgcgga acgggaatga aacctgacga
     5821 cctgatcggt gcatgggctt gtagcgcgtg tcacgatgaa atcgaccgac gtacccataa
     5881 tctcgacaac aaagacgcca gactttacca cctcgaaggc gtgatcagga cgcaggcgat
     5941 actgctgaag gaggggaaga ttaagtcatg aacgaatatc agtttgtgct tccatacccg
     6001 ccgtcggtga atacctactg gcgaagacgg ggaagccaat attacataag cgataaaggc
     6061 cagaaatacc gaaaagacgt tcagcaaatc atccgccaac tcaagttaga cattttcacc
     6121 aaatcacgac tccgcatcaa agtcatcgca gacgttccag actcccgccg ccgcgacctc
     6181 gataacatcc tgaaaggttt actcgactca cttatccacg ccggatttgc ggaagacgac
     6241 gagcaattcg atgacattcg cgtaattcgt ggtgtgaaag taccaggcgg acggcttgga
     6301 ataaaaatca ccgaactgga gaacgtatga acgccacaat tcaaacgata ccagagcttc
     6361 ttatccagac acgaggcaat cagaccgaag tggctaggat gctttcctgc gcaagaggaa
     6421 cagtgcttaa gtacaaccga gacagcaaag gtgagcgtca cataatagtt aacggcgtcc
     6481 tgatggtcaa acagggcaaa aggggaaggc catgagactc gaaagcgtag ctaaatttca
     6541 ttcgccaaaa agcccgatga tgagcgactc accacgggcc acggcttctg actctctttc
     6601 cggtactgat gtgatggctg ctatggggat ggcgcaatca caagccggat tcggtatggc
     6661 tgcattctgc ggtaagcatg aactcagcca gaacgacaaa caaaaggcta tcaactatct
     6721 gatgcaattt gcacacaagg tatcggggaa ataccgtggt gtggcaaagc ttgaaggaaa
     6781 tactaaggca aaggtactgc aagtgctcgc aacattcgct tatgcggatt attgccgtag
     6841 tgccgctacg ccgggcgcaa gatgcagaga ttgccacggt acaggccgtg cggttgatat
     6901 agccaaaaca gagcagtggg ggagagttgt cgagaaagag tgcggaagat gcaaaggcgt
     6961 cggctattca aggatgccag caagcgcagc atatcgcgct gtgacgatgc taatcccaaa
     7021 ccttactcaa cccacctggt cacgcactgt taagccgctg tatgacgctc tggtggtgca
     7081 atgccacaaa gaagagtcaa tcgcagacag cattttgaat acggtcacac gttagcagca
     7141 tgattgccac ggatggcaac atattaacgg catgatattg acttattgaa taaagttggg
     7201 taaatttgac tcaacgatgg ataaatgcac tcgttaaata aagtcctgag ttaatagctc
     7261 ggggcttttt gcgttttaag cacggccttt ctgaaagcac atcaaaccaa ataccagaca
     7321 gacaaaaata atcaccttat ccgctgtggc tacggtgcgg tgtgctttgc ataaaagaaa
     7381 accagcgcaa tggctggctt cgtgaaagcg ggtggcagga ggttgcacta acaacctcat
     7441 gccgttttgc ccgtgcatat cggtcacgaa caaatctgat tactaaacac agtagcctgg
     7501 atttgttcta tcagtaatcg accttattcc taattaaata gagcaaatcc cctcaatgaa
     7561 ggggtagagc atgtaccgta tggacaaaat cagagaatgg ttcagttaca gcttcggagg
     7621 actgactgcg atgggtggca ttctctccct gaatgactgg gctgtcatca ttggtattct
     7681 ttgtactgtc ggcacatttg gcatcaactg gtactacaag cgcaaagagc gcgaggacag
     7741 attgaatggc aatgtcaccg gcactacgaa atagcgtaat agcggcgata agtggcgggg
     7801 ctattgctat agcatctgtg ttaatcactg ggccaagtgg taacgatggt ctggaaggtg
     7861 tcagctacat accatacaaa gatattgttg gtgtatggac tgtatgtcac ggacataccg
     7921 gaaaagacat cgtgctcggt aaaacgtata ccgaagaaga atgcaaagcc ctcctgaata
     7981 aagaccttgc cacggtcgcc agacaaatta acccgtacat caaagtcgat ataccggaaa
     8041 caacgcgcgg cgctctttat tcgttcgtct ataacgtggg cgcaggcaat ttcagaacat
     8101 cgacgcttct tcgcaaaata aaccagggcg atatcaaagg cgcatgtgat cagctacggc
     8161 gctggacata cgctggcggt aagcaatgga aagggctgat gactcgccgt gagattgagc
     8221 gtgaagtctg tttgtggggt cagcaatgaa cagagtaacc gcgattatct ccgctttggt
     8281 tatctgcatc atcgtctgcc tgtcatgggc tgttaatcat taccgtgata acgccatcgc
     8341 ctacaaagaa cagcgtgata aaaaagtcag tgagctgaag caggcgaccg ccaccattac
     8401 tgacatgcag caacgccagc gtgctgctga tgcactcgat gctaaataca cgaaggagtt
     8461 agctgatgcg aaagctgaaa atgatgctct tcggcgcaat cttgataatg gtggcagggt
     8521 gctcgtcaaa ggaaaatgcc ctgtgccatc ctcagccgaa acctccggcg cctccggcat
     8581 gggcaatgat gccaccgtcg aactctctcc agttgctgga cgaaacgttc tcggtgtccg
     8641 ggacggaatt atccgcgacc aaacagcact gagaacgctt caggaatata tcaggacgca
     8701 atgccttcga tgatagcgat aattttactc atcatccttc acatctggct ctgtagacag
     8761 ggtgatgatc acttctggag tgaatccaga ttaaacatct cattgctgat gcttgatatt
     8821 gagcattttg cgcgcggtaa ggggctgcgt tgagataaga gccagtcatt acaaatacca
     8881 ggatttagcc tcgcattcgc ggggcttttt attgccatta caaaagccac ttcctacaga
     8941 gtggctttga taatggctta taccctacac gggataactt aactgatatc ccttttaacg
     9001 gataaacgga gccaacaatg gcagagatta ttcccatgac tgaagaacag aaattccagt
     9061 tagagattta caaactggtc atgaaccaga acgcagccgc ggaggaagca tttcaattca
     9121 ttggcactga cgagctgaag cttgagctat tcaaaattca cttccagtca ggcggcgcta
     9181 attcagatat cacgatccgc acattcgaag cggtgcgtaa atctaaggaa gcgttagacc
     9241 tgttcactac cggagcataa acatggcgcg cccaacaaag tatcaagagg cgtatgccga
     9301 acaggcacgc aaactgtgct tgctgggcta caccgatgca gaacttgctg atttctttga
     9361 agtcagtgag tcaactatta acaagtggaa gcttgattat cctaagtttt cggagtccat
     9421 aaaaaagggt aaggccgtag ctgatgcaga agttagtgac cgtctttatc aacgcgctat
     9481 gggcttcgtg gctccagaca tcgatattcg tgttattgaa aacagaattg tcgaaactcc
     9541 gcttgagaag tattacccgc ctgatacaac cgctgccatc ttctggctta agaaccgaca
     9601 gaaggataaa tggcgcgaca aggttgatca cgaattaaca ggcaaagacg gcggcgcaat
     9661 ccagattgaa acatcaccga tgagcactct attcggaaaa tgacctcgat taatcctatc
     9721 tttgaaccgt tcattgaggc gcatcgctac aaagtcgcca aaggcggtcg aggtagcggt
     9781 aagtcatggg caattgctag gctgcttgtt gaagcggcgc gtcgtcagcc agtgcgtatt
     9841 ctctgcgctc gtgaactgca aaacagtatc agcgattcgg taatccggtt gcttgaagac
     9901 accatagagc gggaagggta ttcgtctgag tttgaaattc agcgttcaat gattcgtcat
     9961 ctcggaacga atgctgaatt catgttctac ggcatcaaaa acaacccgac gaagattaaa
    10021 tcgctagaag gcattgatat ttgctgggtg gaagaagcgg aagcggtaac aaaggaatcg
    10081 tgggatatcc tgattccaac catccgcaag ccgttttccg aaatatgggt gagctttaac
    10141 ccgaagaaca tactcgacga tacctatcag cgattcgttg taaatcctcc cgatgatatt
    10201 tgcctgctga cggtgaacta caccgacaac ccgcattttc ctgaagttct ccgtctggag
    10261 atggaagagt gcaaacgcag aaacccgaca ctgtatcgtc acatctggct tggtgagcca
    10321 gtaagcgcaa gtgatatggc aatcatcaaa cgtgaatggc ttgaagccgc aaccgatgcg
    10381 cacaagaaac tcggatggaa agcgaaaggc gctgttgtct ctgcgcatga cccatcagat
    10441 acagggccag atgctaaagg ttatgcatcg cgtcacggtt cggtagttaa gcgcattgcc
    10501 gaaggtctgc tgatggacat caacgagggt gctgactggg ctacttcgct ggcgattgaa
    10561 gacggcgctg accattacct gtgggatggt gatggtgttg gtgccgggct acgcagacag
    10621 acaacggaag cgttctccgg caagaaaatc accgccacga tgttcaaggg cagcgaatcg
    10681 ccattcgatg aagatgctcc gtatcaggcc ggagcatggg ctgatgaagt cgtacagggc
    10741 gacaacgttc gcactattgg cgatgtattc cgcaataagc gagcgcaatt ctattacgcg
    10801 ctggctgaca ggctgtatct gacatatcgg gcggttgtcc acggtgagta tgcagacccc
    10861 gacgacatgc tgagtttcga caaagaagtg ataggcgaga agatgctgga gaagctgttt
    10921 gcagaactga cgcagattca gcgcaaattc aataataacg ggaagctgga gcttatgact
    10981 aaggtcgaaa tgaagcagaa gctcggtatt tcatctccta acctggctga tgcgctgatg
    11041 atgtgtatgc attgcccggc attggtccgc gaagaaacag aaatatacgt tccctcatcc
    11101 tccggttggt aaacatggca gagacattag agaaaaaaca tgagcggatc atgctcaggt
    11161 ttgaccgcgc ctattctcca cagaaggaag tgcgcgaaaa gtgcattgaa gctacgaggt
    11221 ttgctcgtgt ccccggaggt caatgggaag gagcaacggc ggctggaact aagcttgatg
    11281 agcagttcga gaagtatcct aagtttgaaa tcaataaggt agcaactgaa cttaaccgca
    11341 tcattgcaga ataccgcaat aacagaataa ccgttaagtt tcgtcctggt gacagagagg
    11401 caagcgaaga gttagccaat aaattaaatg gtctgttccg tgctgactac gaagaaactg
    11461 atggcggtga ggcttgcgat aatgcatttg acgacgctgc tactggtggt ttcggttgct
    11521 tccgtttgac gtcgatgctg gtcaatgaat acgaccccat ggacgatcgt cagcgtattg
    11581 ctattgaacc aatatacgac ccgtcgcgct ctgtgtggtt tgaccctgac gctaagaagt
    11641 acgacaaatc tgacgcgttg tgggcgttct gtatgtattc gttgtcacct gaaaaatatg
    11701 aggctgaata cgggaagaaa cctcctactt ctctggatgt aacgtctatg accagttggg
    11761 aatataactg gtttggtgca gatgttattt acatagcgaa gtattacgaa gttcgtaaag
    11821 agtctgttga cgtcatcagt tatcgacatc caatcactgg agagattgca acatacgaca
    11881 gtgatcaggt tgaagatatt gaagatgaac tggcaatagc tggatttcat gaagtggcaa
    11941 ggcgctcagt gaagcgccgt cgtgtgtatg tatccgtagt ggatggtgat ggtttccttg
    12001 agaaacctcg acgtattcct ggtgagcata tccccctcat cccggtttat ggaaaacgct
    12061 ggttcattga tgacattgag cgtgtcgaag ggcacattgc aaaagcaatg gatccacagc
    12121 gtttgtataa ccttcaggta tcaatgctgg ctgatactgc agcgcaagac cccggtcaga
    12181 tccctatagt tggcatggag caaattcgtg gacttgagaa gcactgggag gctcgcaaca
    12241 agaaacgacc agcgttcttg ccgttgcgcg aagtgagaga taaatctggc aacattatcg
    12301 ctggagctac cccggcagga tatacacagc ctgcggttat gaatcaggca ttggctgcat
    12361 tactacagca aaccagtgca gatattcagg aggttacagg cggcagtcag gccatgcagc
    12421 agatgccaag taatattgct caggaaacgg ttaacaactt gatgaacaga gcagatatgg
    12481 cttcgtttat ctatctggac aatatggcga aaagtcttaa acgcgctggt gaagtatggc
    12541 tgtcaatggc gcgtgaagtg tacggttcag agcgtgaagt gcgcatcgtt aacgaagatg
    12601 gaagtgatga tatcgctgtc ctgagcgcac aggttgttga caggcaaaca ggggctgttg
    12661 ttgctttaaa tgacctttct gtcggtcgat acgatgtgac ggttgatgtt ggaccaagct
    12721 acacagcacg acgtgatgca acggtttctg tactgacaaa tgtccttagc tctatgcttc
    12781 caacagaccc aatgcgcccg gcaattcagg gtattattct ggacaatatt gatggcgaag
    12841 gccttgatga cttcaaagag tacaaccgaa accaactgct gatatctggc attgcaaaac
    12901 cacgcaatga gaaagagcag cagattgttc aacaggcgca aatggcagca caaagccagc
    12961 caaatcctga aatggttctc gctcaggcgc aaatggtagc agcgcaggca gaagcgcaaa
    13021 aagcaactaa cgaaactgct caaactcaaa tcaaagcatt tactgcccag caggatgcga
    13081 tggagagtca ggcaaacact gtctataaac tggctcaagc cagaaacatc gatgacaaag
    13141 cagtgatgga ggcaatacgc cttctgaaag atgtcgccga gtcacaacaa cagcaattcc
    13201 agtcaccacc acagtcaccg gcagacttaa tgccgagtta accaggagta atcaatggaa
    13261 aacgaactga tcatcgacgg tcaggttatt gacctgtctg aaacacagga aaatgcagaa
    13321 gaaaccatca tccaaacaga gtcacagcct gagaatgaaa gccaggatga caacggtaaa
    13381 gaggtggcaa ctgagcctga aaaaaccgaa gagacaccag aagattacgc cttgcgtatt
    13441 ggtgatgaag aaattcagct taacgctgac gatgatgatc acattgacgg gcaacctgcg
    13501 ccgcaatggg tgaaagatct tcgcaaaggc ttcaaagaaa cacagaaaga aaaccgtgag
    13561 ttgcgccgcc agcttgagga agcattagcc aagccagcgg aacatcagca accacaacca
    13621 gacgctattc caccaaaacc gactcttgag tcgtgtgatt atgacgaaca ggcgtttgaa
    13681 caggcattga ctgattggca tgagaaaaaa ggccgtgtcg aacagcagca gcaacaaaaa
    13741 ctacgtcagc aacaggaata ccagcagcgt ttccagcaaa gggtagaagc gcataaacaa
    13801 cgggcagcca aacttcctgt gaaagattat caggaaatgg aagccattgt tcttagtgag
    13861 ctaccaccaa ttcagcagga aatcatcatt cactgtgcag acgaaggctc tgaactactc
    13921 gcctatggct taggcaagag ccagcaatta cgccagcgtg tagccgctga gacagatcca
    13981 attcgcgcag cattcctctt ggggcagatt agcaaacagg taagccttgc tccaaaacca
    14041 aagaaagcca tcaagccaga gccggaagta cgtggtggcg gtgctgatgc gaaacaagac
    14101 gaattcaaca aattatgccc cggcgcaaaa atcgaataag gaaaagataa atgcctaaca
    14161 atctcgacag taacgtcagt caaatcgttc tgaaaaaatt ccttccgggt tttatgtcag
    14221 atttagttct ggcgaaaacc gtagaccgtc agttgctggc aggtgaaatc aactccagca
    14281 ctggcgatag cgttagcttt aaacgtccgc atcaattctc atccctccgt actcccactg
    14341 gtgatatttc agggaaaaat aaaaacaacc tgatctcagg taaagctacg gggcgtgtag
    14401 gtaactacat cactgttcct gttgaatatc agcaactgga ggaagcgatc aagcttaacc
    14461 agctggaaga aattctcgcg ccggttcgcc agcgaatcgt taccgacctt gaaacagagc
    14521 ttgctcactt catgatgaat aacggtgcgt tgtcacttgg tagccccaat actccaatca
    14581 ccaaatggtc tgatgttgcg cagacggcat ctttcctgaa agacctcggc gttaatgaag
    14641 gtgaaaacta tgctgtaatg gatccatggt ctgcacagcg acttgctgat gcgcagactg
    14701 gtttgcatgc ttcagatcaa ttggttcgta ctgcatggga gaacgcacag atcccaacca
    14761 attttggcgg cattcgcgca ctgatgtcta atgggcttgc ctctcgtacg cagggggcat
    14821 ttggcggaac actgacagtc aaaacacagc caactgttac ctataacgca gttaaagact
    14881 cataccagtt cactgtaaca ttgaccggag cgacagccag cgttacaggt tttctgaaag
    14941 ctggtgatca ggtcaaattc accaatacct actggctgca acagcagacc aaacaggcgt
    15001 tgtataacgg agccacacca attagcttca ctgcaacggt tactgctgat gctaattcag
    15061 acagcggtgg cgatgtgacg gttacgcttt ctggtgttcc gatttatgac actacaaacc
    15121 cgcagtacaa ctctgtaagt cgtcaggtag aggcaggcga tgccgtatct gtagtaggca
    15181 ctgctagcca gacaatgaag ccaaacctgt tctataacaa gttcttctgt ggacttggct
    15241 ctatcccact gccgaaactg cacagtattg attctgctgt tgcaacatat gaaggtttct
    15301 ccatccgcgt acataaatac gcagatggcg atgccaacgt gcaaaaaatg cgcttcgact
    15361 tactgcctgc atatgtgtgc tttaaccctc acatgggcgg tcagttcttc ggtaatccgt
    15421 aataacaagg ggcttacgcc ccttttatgt tttaaggaaa caatatggat cggatgagtg
    15481 tattccttgc cgcagataac gaatccgggc atgtacaggc cgttatcgca gaaaaagact
    15541 tccagttttt cgaaaggttg ggctttgttg cctcagttga tgaattgaaa ccgaccagta
    15601 agcgaggtcg taaggcggca gacaatggca acagtactga caaagggtga gatcgtcctt
    15661 tttgcgcttc gtaagtttgc tattgcttct aatgcatcgc tgactgatgt tgagccgcaa
    15721 tcaattgaag atggtgtaaa tgatctggaa gatatgatgt ccgagtggat gattaacccc
    15781 ggcgacattg gttacgcttt cgcaactgga gatgatcagc cattaccaga tgatgagtca
    15841 ggtcttccaa gaaaatacaa acacgcagta ggctatcagt tattgctgag aatgctatct
    15901 gattacagcc ttgaaccaac tccgcaagtt ctcagtaacg cccaacgctc atatgatgcc
    15961 ttgatgaccg acactctggt tgttccttca atgcgacgac gtggagattt tcctgtagga
    16021 cagggtaata aatatgacgt gtttacatct gactgatatt atccaggcga tctccctctg
    16081 attgatggcg atatcccaaa cgcataggtg aataaatgcc gattcagcaa cttccgctta
    16141 tgaaaggtgt cggcaaagac ttccgaaacg ccgactatat cgactatctg ccagtgaata
    16201 tgttggctac accaaaagaa atccttaaca gcagcggata tcttcgctca ttcccgggca
    16261 ttgccaaacg ttctgatgtg aacggcgtat cgcgtggcgt cgagtacaac atggcgcaga
    16321 atgctgttta tcgcgtgtgt ggtggcaagc tgtacaaagg cgaaagtgaa gtcggtgacg
    16381 tcgccggaag tggtcgtgta tcaatggcgc atggtcggac atcacaggcg gtaggcgtta
    16441 acgggaaact ggtcgagtat cgctatgatg gcacggttaa aaccgtctca aactggccta
    16501 cagacagcgg attcacgcag tatgagttag gctcagtacg cgacattacg cgcttacgtg
    16561 ggcgttatgc gtggtcaaaa gacggcactg attcatggtt tatcactgac cttgaagacg
    16621 aatcacatcc tgaccgatac agcgcacaat atcgcgcaga atcgcagcct gacggcatca
    16681 tcggcatcgg aacatggcga gacttcatcg tctgctttgg ttcatcgacg attgaatatt
    16741 tctccctgac tggcgcaacc accgttggtg ctgctttgta tgtcgcacag ccatcactga
    16801 tggtgcaaaa aggtatcgcc gggacttact gcaaaacgcc gtttgctgat tcctatgcgt
    16861 ttatcagcaa tccggcaaca ggtgcgccgt ctgtgtatat catcggctcc ggtcaggtgt
    16921 caccaatcgc cagcgcgagc attgagaaaa tcctccgctc ctacactgct gatgaactgg
    16981 ctgatggcgt gatggaatcg ttgcggtttg atgcgcatga gttgctgatt atccatcttc
    17041 cgcgccatgt tctcgtgtac gacgcatctt caagtgccaa tgctccgcaa tggtgtgtgt
    17101 tgaaaactgg cttatatgac gatgtgtacc gcgctatcga cttcatttat gaaggcaatc
    17161 agataacgtg cggcgataag ctggagtccg tgaccgggaa attgcagttc gatatcagca
    17221 gtcagtacgg gcttcaacag gaacacctgc tgtttactcc gttgttcaaa gcggataacg
    17281 ccagagtttt cgactttgag gttgaatcgt caactggcgt tgcgcagtac gctgaccgcc
    17341 tttttctctc tgcaaccact gacggcataa attacggtcg tgagcagatg attgagcaga
    17401 atgaaccgtt cgtttacgac aaacgcgttt tgtggaagcg agtagggcgc atcaggaaaa
    17461 atgtcggttt caaattgcgc gttatcacga agtcacctgt aactctgtct ggctgccaga
    17521 taaggattga gtaatggctg attcgaatct caacacccct gttattgtgc aggcgacgcg
    17581 gctcgataca tcaatccttc cacgcaatat attcagccag tcttacctgt tgtatgtcat
    17641 taatcagggg gctgatgtcg gcgcaattgc cgggaaggca aatcaggctg gtcagggcgc
    17701 ttacgatgcc caggtaaaaa acgatgaaca ggatgtcgaa ctggctgatc acgacgcaag
    17761 aatcaccgca aacacaaaag cgataaatct ccttgaggtc aggttaacaa ccgccgaagg
    17821 gaagatagtc gtactgcgta gcgatgttga ttacttgctt gatgaggtta tcgatattca
    17881 ggcgcatctg gtcactgttg accaaagact ggatggcgta gaaagcgatg tatctgacat
    17941 taagagtgat tacgtatcga aaaccgtaac agaatcgcag tctcttgcgt caccgctgga
    18001 tgtaaaaaca tcatattcag ttgatggaat tcaggttgtt ggagcaagaa ataccggatg
    18061 gactgcagcc acaggtatac ctcttcttgg ctcattcaac gctaaccagt catacacggt
    18121 cggcactacg tacacacaat ccgaagtcgc ggctctcgct acaggtttgc agcaggcgcg
    18181 gcagcgtatt ctggcgcttg aaacagcact tagattacat gggctgattg actgatgatt
    18241 acattcaaac caacgcgaaa catcgacctg atagaagctg tcggaaatca ccctgacatt
    18301 atcgctggta gcaacaacgg tgatggatac gactacaaac ctgaatgccg ttactttgag
    18361 gttaacgtgt acggtcagtt tggcggcatt gtttactatc aggagattca gccgctgaca
    18421 ttcgattgcc acgccatgta cctgccagag gttcgtggct tcagcaagga aatcgggctg
    18481 gcgttctggc gatacattct gactaacacc accgttcagt gcgtcacatc gttcgccgca
    18541 cgcaaattcc gccacgggca gatttactgc gcaatgattg gccttaatcg tgtaggaacc
    18601 atcaagaaat acttcaaagg cgtggatgac gtgacgtttt acagcgccac acgcgaagaa
    18661 ctaatcgact tcctgaatca cgggagatag ccatgttata tgcatttaag ctgggcagaa
    18721 aactgcgcgg cgaggaacct tattgccctg aaaaaggcgg gaaaggtggc tccgataaaa
    18781 gtgcaaagta tgccgcagaa gctcagaagt atgccgcaga cctgcaaaat cagcagttca
    18841 acaccatcat gaataacctg aagccgttta ctcctctggc tgagaagtat gtcggcagcc
    18901 tcgagaactt atcgtctctg gaggggcaag gtcaggcact taaccagtat tacaactctc
    18961 agcagtataa agaccttgca ggtcaggctc gctatcagag tctggcggca gcggaagcaa
    19021 caggtggatt gggttccacc tcaaccagta atcagttagc aacaatcgca ccaacgcttg
    19081 gtcagcaatg gctatctggg caaatgaaca actaccagaa tctggcaaat attggtcttg
    19141 gcgcgcttca ggggcaggca aatgccgggc aaacatatgc caacaacatg agccagattt
    19201 cacagcaaag cgcagcactg gcggcggcaa acgccaaccg accctccggc cttcaatctg
    19261 caataggcgg ggctgcctct ggagcaattg ctggagcaca gcttggcagc attgtacctg
    19321 gtattggtac tggtattggt gccgctgttg gcggcggtct tggtctgctt ggttcactgc
    19381 tttattaagg ggtaatcaat ggctacgtgg caacagggta ttaattctgg tggttttctg
    19441 gctggcatcg gtacgcaaaa tgagaatgcg ccaaaggcaa gcgacattaa cgcaacgctt
    19501 ggtctgatcc gcgaaaacaa tgaactggct cgctcaggtg caaataacgt tggtctgacc
    19561 gcgttacgtg gtctggctgg agttgctgat atttacaatc aggaacagca acagaaagcg
    19621 attagtgcgt tcaataaggt tcacgctgat gcatgggctt ctggtgatcc atcgggacta
    19681 tttaagtttg cccaggaaaa tccagcgttt gttgcgcagg cacaacaggc gttttccggt
    19741 cttaatgagc agcaacgcaa cgatatgggc gatttagcca tgagggctaa cgtcgctctt
    19801 tctcagggac cggaagccta cagtaaattc attactgaca acaaggacag gttaaatcgc
    19861 gtgggggcga atgctgactg gatgattcag acaggtattc agaatccaga gcagctatca
    19921 cacatgctga ctactatgtc tctcggtgcg cttggaccag aaaaggcgtt tgctgttcag
    19981 gataagatgg ttggtcgcca gcaggagcag caaagaatta acgaaaccat tcgcaataat
    20041 gacatgacga atgcgagggc tattaggggg caggatcttt cctataaggc tcaaatggca
    20101 agactgaatc acgacaagta tgtgtttaag cagtcacagg cggcccttga aagagcagga
    20161 caacttcagg atatggatgt tttgtctctt aactcacaga tagcagcgac gggaattgat
    20221 cctctaaccg gtaaagctgc aacgtcagcc agaatgtctc aggctaagag atggcttgat
    20281 ggcaacaata attacaacaa tgcgttgatt actggtgagc gagggataga gaaaattgat
    20341 tctttgcttg gtaagaagga gcttgaaggt atcggtcgct tcgaaggaag aaatatagat
    20401 ggcttcacaa gtgctgaagg gcttgcaaac cgtaatgcga tagaagaatt aaagtcgggt
    20461 gcgtttgtcc agaacgtgca ggctatgcga ggtatgggta gcctctccaa tgctgaaggc
    20521 caaaaactgg aaaacctgat cgcgaaactc gatataacac agcctgaaga ggtcgtcaga
    20581 aaacagttat ctgaaatccg atcgcaatat tctgtatttc aaaaggttgc agcaagggag
    20641 gctgaatcaa tgggatatag ttcatcaggt tatgacacat atgttagtga gcgaaaatca
    20701 ggaagcgaca gcaataagtc cggtttctcg tctttatggg gtgattaatg gctaaagcat
    20761 ggaaagatgt tatcgcttct ccacagtatc aggcgttaac tgaagaacag aaagcacagg
    20821 ctcaagcgca atattttgat gaggttgttg cccctaaggc tggtgacaaa tgggctgaag
    20881 caagagatca gttttatgca gcataccctc cgcctcagca gcagaaagaa gaaccatcat
    20941 tgatgcaaca agctggtgat tggctcacag gtggtcaaag tgcagggcaa attgcagagc
    21001 aggctggtcg tggtctggta aacataccat tcgacgtatt acagggcggc gcaagcctga
    21061 ttaatgcaat cagccagggg cttggtggcc ccaaggtttt ggatgatgtt tatcgtccag
    21121 tcgatcgacc gacagaccct tacgcgcaag ctggtgaaac aattggcggg tatttagttc
    21181 caggagttgg aacggcagga agcatggcta ttggatcact ggcagaggcc gcaaatcaga
    21241 aaggcgattt cgcacaaaat gcaactaaaa atgccggagt taaccttgcc gctcaggggg
    21301 ttctttccgc agcagcaaag ggaatagggc gtggaattac tgctgttcgt ggcgaaatat
    21361 caccagcaga tcagcaattg ctcaagcgtg ccgctgcggc agatgtacca gttatgacat
    21421 cggatgtagt tcctccaaaa acaaaacttg gcaatcaact gcagggttac tcagaaggag
    21481 tcatagctgg gactggacca atgagagccg cacagcagga tgctagaacc aagcttgtta
    21541 atcgcttcac cgaaaaatac ggcgactacg atccatctgt agtcgttgat agtctaaagt
    21601 caggcgttgc aagggaaaaa tcgttagcca agtcaaaact aaacaacctg tcaggaagaa
    21661 tggttggaaa gccagttgat acaagtggcg ccataagagc tatcgacgga gcagtaaacg
    21721 aacttgggaa acttaaaggt gtttctgaca cccagaccat ttctgcgctt aatgattata
    21781 agaatgccat tcaggagata acacatggag atgatgcctt tgagttactt gataagctga
    21841 gaactcagtt ccgcattgac gtaaaaggcg atcgtacagt tctgccatca atgtcgcaaa
    21901 caatggtcga cagggtctac aactcgctaa ccaatagcct tagtaaatct atagcgaaag
    21961 gacttagccc aaaagatgct tcagcatgga gagcgggaaa agctgattat gcaaaaatgg
    22021 caacacatgc aactcaaacg cgccttaaaa acgttctaaa caaaggagat ttaactcccg
    22081 aggctgtaaa taccattgtg tatggacaat atgggtcaga tatagctcga ttgtacggga
    22141 aactcgatca aaaaggtaaa gacatgctaa gggcggcata tatcagcaaa atagctgaca
    22201 aggtaggtga cagccctcag aaaatgatga ccgagcttgg caagctgcaa aaacaagcaa
    22261 atggtcaggt gtttaaaact gtatttggtg ggaagaacgg aaaagagata gaggggatgt
    22321 tatctattct cgatgctacc aaaagagcat ctgaggctaa tgttgtgacg aagactggca
    22381 tgacactcgc gcctttggta agggttattg gtaacctaaa aaccggaggc gcgctattgg
    22441 ctggggaaac agggattggc cttatgtcga gggtttatga aagccctatg gctaggaatg
    22501 cgctcttacg tctggcaaac actaaagctg gaacgccagc ttatgaaaga gcgctgaata
    22561 acgccgcaaa tgccatcaga ccaatacttg ccaatgatgc aactgatcgc taagggttga
    22621 tgtcatggat gttgttaatt gcgaatggaa tcgcggaact ctttgcttga tagaaaagga
    22681 tctgttctcc ttaactcatc atagttttta ttaaactttc ttgcttcacc tgggtatctg
    22741 ttgaacaaaa cattgcagaa aaaagagagc aataactcac atatgaatgc taataccctg
    22801 aaaaataaag gtataaaaag aagggaaggt tcattgaagt ctctattcca aacaaaatag
    22861 acaatcatcc cgacatacca aattgatatc cctatactga tgattgttcc aacagaggta
    22921 actacgatcc cgattaagtc ggacagtctg tttgccagta actcatttga gtatcttgca
    22981 atcatatacc gccaacattt tgcaatatca tcctgatctg tagagtcttc tggaatagct
    23041 aggattctac atattgaagt agctcttttt ttctcaactc tttttgagag ccatccatta
    23101 aagagataca tcaacacctg cgttgctatt cctgcaccaa gtgatatcaa agacatcccc
    23161 aaaaatcgcc atcctggaga tagtgactgg tactgccaaa caaaaaaaat taaaaaaccc
    23221 aatgagccta tgacataggc tgatgtatcg ctatatatat ctattttttt cattgagtag
    23281 ccactcctta gttttgagca gaacaccgga tgataatgtt ttgttatgtg tcttttggga
    23341 ttctatccag taactcgttg gaaattttat caagtaaaga ttttatttgt ttaagatcta
    23401 atgatttgtc tactgatgag acgtcatatg ataacgcatc ctcaagaatt tgaactatc
//
LOCUS       DAFNBJ010000070        23378 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000070 DAFNBJ010000000
VERSION     DAFNBJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.070"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..236
                     /locus_tag="L2X49_002597"
     CDS             <1..236
                     /locus_tag="L2X49_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4797669.1"
                     /translation="NYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRH
                     VGCSPSDYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(279..1430)
                     /locus_tag="L2X49_002598"
     CDS             complement(279..1430)
                     /locus_tag="L2X49_002598"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBN4797670.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1444..3039)
                     /locus_tag="L2X49_002599"
     CDS             complement(1444..3039)
                     /locus_tag="L2X49_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HBN4797671.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3184..3942)
                     /gene="aroD"
                     /locus_tag="L2X49_002600"
     CDS             complement(3184..3942)
                     /gene="aroD"
                     /locus_tag="L2X49_002600"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBN4797672.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(3973..4839)
                     /gene="ydiB"
                     /locus_tag="L2X49_002601"
     CDS             complement(3973..4839)
                     /gene="ydiB"
                     /locus_tag="L2X49_002601"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBN4797673.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4851..6116)
                     /locus_tag="L2X49_002602"
     CDS             complement(4851..6116)
                     /locus_tag="L2X49_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4797674.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6343..7557)
                     /locus_tag="L2X49_002603"
     CDS             complement(6343..7557)
                     /locus_tag="L2X49_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4797675.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYLCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7657..8013)
                     /locus_tag="L2X49_002604"
     CDS             complement(7657..8013)
                     /locus_tag="L2X49_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBN4797676.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8149..8256)
                     /gene="rprA"
                     /locus_tag="L2X49_002605"
     ncRNA           complement(8149..8256)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="L2X49_002605"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8442..9554)
                     /gene="ydiK"
                     /locus_tag="L2X49_002606"
     CDS             complement(8442..9554)
                     /gene="ydiK"
                     /locus_tag="L2X49_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBN4797677.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            9943..12999
                     /locus_tag="L2X49_002607"
     CDS             9943..12999
                     /locus_tag="L2X49_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBN4797678.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIKINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            12996..13406
                     /gene="menI"
                     /locus_tag="L2X49_002608"
     CDS             12996..13406
                     /gene="menI"
                     /locus_tag="L2X49_002608"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBN4797679.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13506..13694
                     /locus_tag="L2X49_002609"
     CDS             13506..13694
                     /locus_tag="L2X49_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBN4797680.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14243..14611
                     /gene="sufA"
                     /locus_tag="L2X49_002610"
     CDS             14243..14611
                     /gene="sufA"
                     /locus_tag="L2X49_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBN4797681.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14620..16107
                     /gene="sufB"
                     /locus_tag="L2X49_002611"
     CDS             14620..16107
                     /gene="sufB"
                     /locus_tag="L2X49_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBN4797682.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16117..16863
                     /gene="sufC"
                     /locus_tag="L2X49_002612"
     CDS             16117..16863
                     /gene="sufC"
                     /locus_tag="L2X49_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBN4797683.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRSQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16838..18109
                     /gene="sufD"
                     /locus_tag="L2X49_002613"
     CDS             16838..18109
                     /gene="sufD"
                     /locus_tag="L2X49_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBN4797684.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18106..19326
                     /gene="sufS"
                     /locus_tag="L2X49_002614"
     CDS             18106..19326
                     /gene="sufS"
                     /locus_tag="L2X49_002614"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBN4797685.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19339..19755
                     /gene="sufE"
                     /locus_tag="L2X49_002615"
     CDS             19339..19755
                     /gene="sufE"
                     /locus_tag="L2X49_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBN4797686.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19904..20908
                     /gene="ldtE"
                     /locus_tag="L2X49_002616"
     CDS             19904..20908
                     /gene="ldtE"
                     /locus_tag="L2X49_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBN4797687.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(20972..21208)
                     /gene="lpp"
                     /locus_tag="L2X49_002617"
     CDS             complement(20972..21208)
                     /gene="lpp"
                     /locus_tag="L2X49_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBN4797688.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21519..22931)
                     /gene="pykF"
                     /locus_tag="L2X49_002618"
     CDS             complement(21519..22931)
                     /gene="pykF"
                     /locus_tag="L2X49_002618"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBN4797689.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         6070 a         5662 c         5894 g         5752 t
ORIGIN      
        1 ttaactatgt tattcaacgg cgtatgacgg aagcaaaatg gtcactcact aatactgaat
       61 tatcacaggc agagatctcc tggcgtgtgg gttatgaaaa tgtcgatcac tttgccaaac
      121 tgtttttgcg ccatgtcggt tgttcgccca gcgattaccg tagacaattt aaaaactgtt
      181 ttgcggaaca agaaatccta tctgaatttc ctcaaccggt aagtcttgtt ggataaatct
      241 ggcggcgcag tttgttaaga agctgcgccg cctgcagatt atttgttctg atagtctttc
      301 aggatctgcc gacctgctac gtaaatcata atttcgtctg taccgccgcc gatacgttca
      361 caacggacat cacgccagaa gcgggagacg cgcgcttcat cggtatagcc cagaccgccc
      421 atgatttgaa tcgcatcatc aatgacttcc attgcggtac gtgcgcaata cagttttgcc
      481 agcgccgcgc tggtgcgcag tgactgatgc tgatcggctt gccatgccac tttcagcacc
      541 atgttgcgca tgttgtcgat cttaatcgcc atcagcgcca gtttttcctg gatcatctgg
      601 ttatgaccaa tgggcttacc aaaagcgata cgttggttgg catagcgggc ggcatcttca
      661 aaggcgcatt cggcaaagcc ggtgctgcgc gcggcgttga tcaggcgctc catctcaaag
      721 ttgtacatca cattgaggaa acccattcct tcttcgccca ccatgtcgct ctcttcaacc
      781 tccacgttgt cgagatagac ttcgcaggtg ctgagcatat gccagccgat tttatgcagc
      841 gggttaatct taatgccggg cttactggag tcgacccacc acagggtgaa ggcttttttg
      901 ggatctttcg gttgcggatc gcgcgccaac accagcatat acgggtactc tttcgcaccg
      961 gtaataaagg ttttttgtcc gttgatgtag accttgccgt ttttacgcgt gtaagtggta
     1021 gtggcactgt tgttatctga gccagcacct ggttccgtca acgccagggc ataggcggga
     1081 tcacccgttt ccagggtgct ttctgccgtt ttacgtagct gctctgcaga accgaaacgg
     1141 cgcatactgt gaatacattg accgttggtg atcaaaaatg ccggagcacc gcattttgac
     1201 acttccatca gcgccagcat ttgggtgacg taatccgcag ggataccacc aaattcttcc
     1261 ggcacgccaa gcatggaaat accgttatcc gccagcgccc gcataaactc acgcggatat
     1321 gtcccgtttt gatcgcaggt gcggaaatac tcttccggaa agttagtcgt aatcagttcg
     1381 cgaatactgg ccagcagcag ttcttgttct tcagttaaag aaaaatccat cattatactc
     1441 ctattaatga gccgcttcag gcaggacaaa acccatcgcc gcatcgataa ataatctttc
     1501 gtccatcagt ttgagttctg gcgaaatcac tggggtgaag tccattttgt cgagaatatc
     1561 tttttgtaaa tcgacgccag gggcgatttc gattaaatgc aggccgtctt ctttcagcgt
     1621 gaatacagca cgttcggtga tgtaacgaac atccagtccc cgttcgagcg cgatttttcc
     1681 gctaaaagta atttccggta gttcccgaat aaatttattt acccgtcctt cctggacgat
     1741 atgcaatttg ccatcggcaa tttctgtttt taaactgccc gcagttaacg tgccgcagaa
     1801 aataattttc ttcgaggtgg cactgatatc aataaatcca ccggtaccca tgattttgcc
     1861 attgaattta tgcacgccga cgttaccgtg ctggtcgact tcagcaaaac tcaaataaca
     1921 aacatccaga ccaccaccgt gataaaaatc aaactgggac gtcatatcca gaatggcgcg
     1981 ggtattcacg ttcgcgccaa aggcgatacc ctgtgaagta attccgccaa tcggacctgt
     2041 ttctaccgtc agaataaagt catcagcaca accttcttcc cgggcgacca ggccaatacc
     2101 gtcagcaata ccgacgccga cattccccac cgcgccttta cgcatttcga ataatgcacg
     2161 ccgcgcaact aatttacgtt ggtttagggg caggctaagt ttggtgctgt catcaagggt
     2221 gaagtcacca gaaataaaac ggttaaccgg cgcaccgcca tacagttggc tttgatccgg
     2281 atcgaccacc acaatatcca ccagataacc cggaatacgg acagatttag gatgcagcgt
     2341 ggctttctta accattttct gcacctgcat catcacaata ccgccgttat tgtgcaccgc
     2401 ctgggcaata accaatgcgt cgagatacat cacctcatct tcaaaagtgg cgtagccttc
     2461 actgtcgcag gtggtagcgc gaatgaaggc gatatctggc gcaatcgctt tgtaatagag
     2521 atattctttg ttatcaaact cgaccagttt aatcaggtct tctttcgtga cttcattcag
     2581 tttgccgcct tgctggcgtg gatcgacaaa tgtcccgatg ccaatatcac taataatacc
     2641 aggctggtgg gctgcggcgg cgcgtaaggt ttgtgtaagt acaccttgtg gatagttata
     2701 agcaataatt ttattttgtt ctgcgagttc agaaatacgc ggcgattgtc cccagtgacc
     2761 acataatgcc catttcacca gaccttcttg cgccagagga ctaataccac ggtcggcgcg
     2821 atcgccaagc cctgtcggac taataatcga taaattacgt ggcgtttgag tctgtttata
     2881 tttatcagca agagcagtaa ttaacgtggt ggcttccaga ataccgccgc cagcgcctaa
     2941 cacacaaagt gttgcttcgt cgggaatata attcaccgct tcctgtgccg acaggaccgg
     3001 cactcgtcca ttaatacgag gtggttttac aggtttcatt gttaattcct gattcaggtg
     3061 tgattttccc tacgccagat ggataattag cgcacagaga ctcacgaaat atttttatta
     3121 aatacggctg aatatatttt tttagttcgg cgggggaggg tattcccgcc gaaatattat
     3181 tgcttatgcc tgatgtaaaa tagttaatac tgtgcgcaaa tcatttaccg agatttgccc
     3241 tggcgcagag gcttttttta ccgcaccaaa agttgccgcc gagccaaata cttcaccagc
     3301 cagacgagaa attacgccag tttttgccat tgacatcgtg atgattggac gatcggcata
     3361 ctgctcctgc atctccaggg tcgcggcaag caacgtcagc acatcgctgg tactttgcgg
     3421 catcagcgca atcttaggaa tatcggcgtc gaaggactgc attttgcgca gacgggcaat
     3481 gatttcttcg gcttccggcg ttttatggaa gtcatggttg gacatgacaa ctttcacatc
     3541 atgcgcgtgg gcgtaggcga cggtttcttt aacctgatca tcgccggtaa ataactccag
     3601 atcgatcata tcaaccaggc cgctgtcgat ggctgcacga ttgagagcaa tataagcctc
     3661 ggtggaaatc gcctgctcgc cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt
     3721 ttctggcatg gtttcacgga gaatttttgc cgccgccatg acagactcca cattggagag
     3781 gtcggcaaag tggtccacac gccattccag aatatcaaag tccgcttcac gataggcgag
     3841 agcttcggat ttcacgcggg cgatatcttt cgccatcagc gagacgatga ttttaggtgc
     3901 gcccgtacca atgacgagat cttttacagt tacggttttc attttttacc ctttctgcac
     3961 gcggtcagcc tgtcaggcac cgaaccccat gacctgttta acatattcca gagggaaatc
     4021 tttgccagtc cataacgtga actgttcagc cccttgccac aacaacatgc cgtatccatc
     4081 aatcgttttg caaccagctt gttgcgcctg ctgcaataac ttcgtcatat gcgggttata
     4141 cacgcattca gtgaccagca gtcccggatg taacagactg atatcattaa ctaatgattc
     4201 attctcaagg ggtttcatac ccacttttgt gccattggtt aaaatgtcgg cggaagccag
     4261 ggcttcagca aaggcttgtt gatcggcgag atcggtaacc gtgacgacac aatcggtgtt
     4321 ttcgttaacc cgctgcgcga aggcgagggc tttatcgaag aactcatccc gacggttaaa
     4381 gagtttaatt tcttttaaac cttcaattgc cccctgcgcg ccaatcgccg ttgaggcacc
     4441 tccggcccct aacagcacca ttgttttgcc tttgatatca aaaccgctct ctttaatggc
     4501 gcgaatatgg cccgtgccgt cggtgttata gccgcgcagg tagccatcat cattaacgat
     4561 ggtgttgatg gcacccacca gtttggctgc tggcgttaat tcatcaacat attcacacgc
     4621 cagttgtttg ttcggcattg atacaccagt tccgcgcatt tttagagctt ttaatccttc
     4681 aattgctccg ggaaagctat cgttatccac ttcgaaggcc atataggtaa atggcaatcc
     4741 cgctttttct aaggctttat tctgcatttc aggcgataaa ctgtggcgga taggataagc
     4801 catcaaccca atcaattcgt attttgcggt aacatccata attaactcct ttaacctcta
     4861 tgcttaattg tgtttagccg ggtgctaaat ttacgctcgc caaaccgcac atcattctca
     4921 ggaataatga aaacgcggta atagcggata aagacaataa ttgcggtaat cagggccagc
     4981 agcgcgaaag taaaatcgag aacaatgata tattgaaggc cgatgttcga cagataaccg
     5041 gtaatcagcg gaataacaaa gttagccagt ccacccatca tcatataaat actggtgact
     5101 ttggctttgc tttttgggaa aaactctgac atgaccgaaa caccgagctg taaaatgccg
     5161 ccagctgctg agaaaccgat aacaaaggcg ccggcattac acaccagtgg agaagggtac
     5221 aggtagataa tggctgctgt tattgttgcc agtgcagagt taaatacatt agcccagatg
     5281 ggccggacca tttttttcag tagtgcggca aaaataaaga cacagaccaa cgagcccata
     5341 ctgtaataag agatggtttt taatgcctca gcttctgaca taccagcaaa agccatcgca
     5401 tatttgggca tccacaccac aatcacataa aaggtcgaga atgcggctac accgaacagt
     5461 accgatgaaa caccttccag ccagactaac ggtttgctgt tcatttgcgg taattcatta
     5521 gctacgctgg cgtccaccaa ctggctgggg aatttgcttt tcaacagcat cagcgtgatc
     5581 agtacaaaca gaatacccgg aataatcagc ccatagccgt accagatatt attgagcaac
     5641 atatagctca ccagcattgg gtagaacatt tgcccaaatg acaccatcgc tttaaccagt
     5701 atgaccgccg aaccagaggc tttcggaaag cattccatga gcgcggggta gccacccgta
     5761 tccagcgctg agttagcgat acctacgcac actgccagac cgtaggcgag agttaaattc
     5821 gggcaagcgg gaataccaaa gaagaatagc agatacatta ttactgccat taatatcacc
     5881 gcccgacgac caaacttatc ggagatcaca ccgaagaata aaatactgat caatcgcccc
     5941 aaaccaatac cggaaattaa gtaggcaatg cccgcgttgt cagtggaaaa cttttccgcc
     6001 agagatgaca tgttttgggc aagcgtaata acactaatgc cgtgcaggaa gtagctgaag
     6061 taaatacaaa gaacagccag gataaatggc gtgctgaaag ccttattttg agacattttc
     6121 acagctcctt gctgaaagat aatttcgtta ccgtacgtat tgttcttcga cggtttacat
     6181 aattactata ctgaagatga ttttccctag gtacatggca taaagtttat gtttccagga
     6241 atattgcatg tcatagtttg tttttgatcg cgattttcct tagttagcgc agaaagagtt
     6301 atttatccgt aagatcttgc atgacctgaa aagaatattg cattacccac ccggagcgac
     6361 attttccttt agcgagtgat gccgcgtttt tttgcggctg cgtaagccga taaacagtgc
     6421 cagtaaaaaa ccgatggcag cgatggcggt atcgaaccac ataatatcgg caatacttct
     6481 ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc ccgcactgta
     6541 atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta acgtcaggcc
     6601 aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata ttatcaccac
     6661 ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa aggtgtacag
     6721 catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa taaatacaca
     6781 aagcagcgaa cccacggtgt agatactgag tagtttaatc gacatagtgt atgacatgcc
     6841 tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg ttgccattga
     6901 gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat gttccgtaga
     6961 gctggtggtt ttctttatga caggtaagcg atgacccgga tggggtggga acgtacaaag
     7021 gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg accaaccgaa
     7081 ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt gtccgctgga
     7141 aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag cttccatcaa
     7201 actgggataa gtgcctgcat cgagaaaact gtttgccatt cctgccagaa agccaaaaac
     7261 ataagcgata atgatgttat tggtgtgcag gatgccaaaa aagaaggcca tatagcagca
     7321 catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag
     7381 caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac ccgcggcatt
     7441 agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga cgcccatacc
     7501 atgcaccagg taattaaaat ataacccaag tgcggtaggg taatagggat ttttcatagg
     7561 ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta
     7621 ttgttgtaac aacgacaatt cctttctctg taaaaattaa caaagccgct ccagatcgtg
     7681 tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat tgccatcagg
     7741 gtagactcgt tgaaatgcgg ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg
     7801 gttatttatt ttctctatga tggcatgaag atgttttttt ctttgctgac gcatagccaa
     7861 cagttgctcg actacacgat caggaatggc acacttgcca ttttcccact gacgccagct
     7921 ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca tggcaaaaat
     7981 atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg aaataaacaa
     8041 taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca gtatcttgtg
     8101 caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc
     8161 ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact caggggattt
     8221 ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg catacggact
     8281 ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt gcgacaggta
     8341 ctaccagctg aatggcggta agcaggaaaa tatggagaag agcaagcaaa tatcaattag
     8401 cggtggttaa caatcaggcg gcagagccgc ctgatgagaa attacttatt cggtttctcg
     8461 atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggcgg gacttcttcc
     8521 acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat aaacagaccg
     8581 atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat cagcggtaaa
     8641 tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc aaccacaccg
     8701 ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag ccagataatc
     8761 gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggaaaggat cattaacacc
     8821 gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc aagcactgcc
     8881 tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg tgccgccagc
     8941 agtacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg aatgccttgt
     9001 gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag cgcacaatgc
     9061 accataaagc gcccgatatg cgccgcttgc ccaacgaacc aggtggtggt ggtgccaata
     9121 taagggcgga ctttcgccat gatcgcagtc ccccccatat ccagcaagtt gtgccagcct
     9181 gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc gggtaacgtc
     9241 atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat actgttaacc
     9301 agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac cagaacggcg
     9361 agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca ggtggcgata
     9421 accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca cagacatgcc
     9481 acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc cctgggctga
     9541 cgtacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg acgcgacgtg
     9601 aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa caggccgatt
     9661 ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact caggcaacac
     9721 gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt cgttcacccc
     9781 agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct gcaactcgaa
     9841 atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct gaaagaatag
     9901 tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc acagatttcc
     9961 caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga gcaacaaggt
    10021 tttaccggcg atacggcgac aagttatgcc gatcgtctga caatgtcgac cgacaacagt
    10081 atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga tgtggcgctg
    10141 atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc ccgcggcggc
    10201 ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat gtcccgccat
    10261 atgaaccgca tcatcaaaat taaccctgaa gagggctggg tgcgcgttga ggccggggtg
    10321 ataaaagatc aactcaatca gtacctgaaa ccgttcggct acttttttgc gccggaactt
    10381 tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc cggtcaggga
    10441 tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt gttgttgggg
    10501 ggcgatattc tcgatacgca acctttaccc gtcgaattgg cggaaacgct gggtaaatcc
    10561 aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca gcaacgccag
    10621 ttaattatcg acaacttccc caaacttaac cgctttctta ccggttacga tctgcgtcat
    10681 gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg ttcagaaggg
    10741 acgctggcct ttattaccga agcgcggctg gatattacgc ccttgcctaa agtgcgccgt
    10801 ctggtgaacg tcaaatatga ctcttttgac tccgcgctgc gtaacgcgcc gtttatggtt
    10861 gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct ggcgcgggaa
    10921 gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa agagatgctc
    10981 gggctgaaca ttgtggagtt tgctggtgat gatgaggcgc tgattgacga gcgagttaat
    11041 gctctctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt gattggctgg
    11101 caggtgtgcc gcgagctggc gggtgttgaa cgtatctatg cgatgcgtaa aaaagccgtt
    11161 ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga ggatacctgc
    11221 gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct cgacagccac
    11281 ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt ccgtccagcg
    11341 ctggatatgt gcgatcctca acaagagatt ttgatgaagc aaatctctga tgacgtagtg
    11401 gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg ttttcgcgct
    11461 gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa agtgaaagcg
    11521 gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga aggtctcgat
    11581 gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca gatccccatt
    11641 gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt atgcttcaac
    11701 tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg gattcattca
    11761 ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgtt tgttggcgga tcgcggcgtt
    11821 gatccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg gacgttaatt
    11881 gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc acacgaagtc
    11941 aaagaggcga tgtcgggctg tctggcctgt aaagcgtgtt cgacccagtg ccccatcaaa
    12001 attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg ttatttacgc
    12061 ccgctgcgcg accacctcgt cgctacggtc gagagctacg cgccgctgat ggcacgcgcg
    12121 ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc ggaaaaacat
    12181 atcggcatgg ttgatttgcc gctgctgtcg gtcccctcgc tacaacaaca aatggtgggg
    12241 catcgctcgg caaacatgac gctggaacag cttgaatctc tcaatgcaga gcagaaagcg
    12301 cgcacagtgt tggtggtgca ggaccccttt accagctatt acgatgcgca agtggtggcg
    12361 gattttgtcc gtctggtcga aaaattaggt ttccagcctg tgttactgcc attttcgcca
    12421 aatggcaaag cccagcatat taaaggtttt cttaatcgtt ttgcgaagac ggcgaaaaag
    12481 acggcggatt tcctcaaccg tatggcgaag ctgggtatgc caatggtggg cgtcgatccg
    12541 gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga acgtggcgag
    12601 tttaacgtct tactggcgaa tgaatggctg gcaagcgcac ttgagtcaca gccagtggct
    12661 acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaagttac cgccttgccg
    12721 ggtgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact ggaaaatgtc
    12781 agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa ccataaaaat
    12841 tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc gcgtaaccgc
    12901 tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg cacgggggta
    12961 cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg aaaatcaccc
    13021 tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat attcgctttg
    13081 aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg acaaagcagc
    13141 ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc ggttccgttg
    13201 ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc aatgctaacc
    13261 acgtccgctc ggcacgagaa gggcgggtgc gcggggtatg caaaccgttg catctcggtt
    13321 cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg tgctgttcgt
    13381 cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg catattgctt
    13441 ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct aaaagcgagg
    13501 atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta cgccgtgacc
    13561 agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa cttgaatttg
    13621 ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt gcctggcgtc
    13681 tgggcgtgca ttgatcctga cttaataccg caagttcgcc aaatcgacct ggattaacct
    13741 ccgtccaggt tcgatgacct tcttaatact gcttatctac ccgcctgata ttgttcaaaa
    13801 ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat tattcttatc
    13861 gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg gggtagaaac
    13921 ccttaagcct gtgtcgcact gacttaaggg ttttcttatt tctgaacgct gcactttttt
    13981 acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac agccttaatg
    14041 ctccttagat aataaccatt atctaacaat gagataccta attcttagcg tgcctgttaa
    14101 cccacacatc agggtctatg cttattaaaa gcaggcaaaa agcatgtttt acggtaaagc
    14161 ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt atacgctgaa
    14221 agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc cacaggattt
    14281 cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg agctggtggc
    14341 aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct gcgcgggctt
    14401 tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt ttgaacacga
    14461 cggcgcgaag ctgtttgtcc cgctgcaagc gatgccgttt attgatggca cggaagtcga
    14521 tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag cccagaatga
    14581 atgtggctgt ggcgaaagct ttggggtata ggcggtacta tgtctcgtaa tactgaagca
    14641 actgacgatg tcaaaacctg gaccggcggc ccgctgaatt ataaagaagg attcttcacc
    14701 cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg cgcaatttcg
    14761 gcgaagcgta atgagccgga gtggatgctg gagtttcgtc tcaacgccta tcgcgcatgg
    14821 ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa ttatcaggat
    14881 tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc gtctgaacct
    14941 ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga ggcggcgttt
    15001 gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc cattttcgac
    15061 tcagtttcgg ttgccactac ttatcgcgaa aaactggcgg agcagggaat tattttctgt
    15121 tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct cggcaccgtg
    15181 gtgccgggga atgacaactt ctttgccgcg cttaatgcgg cggtagcctc tgacggtacg
    15241 tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta ttttcgcatt
    15301 aacgcggaaa aaaccgggca gtttgagcgt accattctgg tggccgacga agacagctac
    15361 gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt acacgcggca
    15421 gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt acaaaactgg
    15481 ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg tgctttgtgc
    15541 gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc gattacgtgg
    15601 aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta ctcagtggcg
    15661 ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat cggtaaaaac
    15721 accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa cagttatcgc
    15781 ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca gtgcgactca
    15841 atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg tcgcaataac
    15901 agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca actgttttac
    15961 tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa cggtttctgc
    16021 aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa actcctcgcc
    16081 atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa agatttacac
    16141 gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg tcccggcgaa
    16201 gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc aacgcttgcc
    16261 gggcgagaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa agatttgctt
    16321 gcgctgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca gtatccggtg
    16381 gagattcccg gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc ggtgcgcagc
    16441 tatcgcagcc aggaaacgct cgaccgcttt gattttcagg atttgatgga agagaaaatc
    16501 gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg tttttccggc
    16561 ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga gttatgcatt
    16621 cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc cgatggcgtg
    16681 aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca acgcattctc
    16741 gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt gaaatccggc
    16801 gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac cgaacagcag
    16861 taacgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac gctccccgca
    16921 agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta aacatgaaaa
    16981 ctggaaatat acgccgctgg aagggctgac caatagccag tttgtcagca ttgcggggga
    17041 gatatcccca cagcagcgtg atgccttagc gttaacgtta gacgctgtgc ggctggtatt
    17101 tgtcgatgga cgttacgtgt cagcactgag cgatgcgact gaaggcagcg ggtatgaagt
    17161 gagcattaac gacgaccgtc agggggtacc cgacgctatt caggcggaag tgtttctgca
    17221 tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg gtcaacggcc
    17281 ggcaaagcca ttgctgttaa tgcatattac ccagggcgtg gcaggtgaag aggtcaacac
    17341 tgcccattac cgacatcatc tggatctggc ggaaggtgcc gaagcaacgg tgatcgaaca
    17401 ttttgtcagc cttaatgatg ctcgtcactt caccggcgca cggttcacta tcaatgtcgc
    17461 agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca gtcaccactt
    17521 tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata gtttcctgct
    17581 gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa acagcacgct
    17641 gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc gtacctggct
    17701 ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg tcagcgacaa
    17761 aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca aaacggatgg
    17821 tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata caaaaccgca
    17881 gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg ggcgtattga
    17941 tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg cccagcagat
    18001 gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg ggcttaaaca
    18061 gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac tttttccgtc
    18121 gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt gccgctggct
    18181 tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc cgaggccgag
    18241 ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag cgctcaggcg
    18301 accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc ccgttcggcg
    18361 gaagagctgg tgttcgtccg cggcacgacg gaagggatca atctggtcgc caatagctgg
    18421 ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga gcaccacgct
    18481 aacattgttc cctggcagat gctttgcgca cgcgttggcg cagagctgcg tgtgatcccg
    18541 ctcaatcccg acggtacgtt gcaactggag acgctgccta cgctgtttga tgagaaaact
    18601 cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc actggcggaa
    18661 atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc tcaggcggtg
    18721 atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt ctccgggcat
    18781 aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt gttgcaggag
    18841 atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag tgaaggcaca
    18901 acctggacca aagcaccttg gcggtttgaa gccggtacac ccaataccgg gggcatcatt
    18961 ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaataacat agccgagtat
    19021 gaacagaatc tgatgcacta cgcgctatca cagctggaat ctgtaccgga tctcactctc
    19081 tatggtccgc agaacaggct tggcgttatt gcttttaatc ttggtaaaca ccacgcctat
    19141 gatgttggca gttttctcga taactacggc attgctgtgc gtaccggaca tcactgcgct
    19201 atgccattaa tggcctatta caacgtccct gctatgtgtc gggcgtcgct ggccatgtat
    19261 aacacccatg aagaagtgga tcgtctggtg accggactgc aacgtattca ccgtctgctg
    19321 ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct gcgtaatttt
    19381 ttacgctgcg ccaattggga agagaaatat ctctacatta ttgagctggg ccagcgtctg
    19441 ccagaattac gcgacgaaga ccgaagccca caaaatagca ttcagggctg ccagagtcag
    19501 gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg cgacagcgat
    19561 gcggcgattg tgaaagggct tattgcggtc gtatttattc tctacgatca gatgacgccg
    19621 caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct cacccaacat
    19681 ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg cgccaaagcc
    19741 gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat tgccggatgg
    19801 tcaatgttta cactgacctg cacaggagtg ttttggtttc aggtgaacat aagcgccatc
    19861 cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac gcgcgtcttt
    19921 gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg cggttgatta
    19981 tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata cggtgcaaga
    20041 aggggataaa aaccttcagg ctattgcccg acgttttgat actgcggcaa tgttgatcct
    20101 tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa ctattccttc
    20161 acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg cagagctgcg
    20221 cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag gtattggatt
    20281 gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc ctaacccaac
    20341 ctggacgcct acggcaggca ttcgtcaacg ttcgctggag cgtggcatta aattaccgcc
    20401 agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc tcgcgcatgg
    20461 taatggcgaa tacctcattc atggtaccag tgcgccggac agcgtcggtt tgcgcgtcag
    20521 ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca gcgtgcggac
    20581 gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac ctaacgggat
    20641 gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg ttcagacaat
    20701 gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg tagatcagaa
    20761 gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga gcagtggagc
    20821 aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg aaccagagca
    20881 agggaatatg ttacgcgcga cgcagtagcg gtaaacggca gacaaaaaaa atggcgcaca
    20941 atgtgcgcca tttttcactt cacaggtact attacttgcg gtatttagta gccatgttgt
    21001 ccagacgctg gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg gaacgcattg
    21061 cgttcacgtc gttgctcagc tggtcaactt tagcgttcag agtctgaacg tcagaagaca
    21121 gctgatcgat tttagcgttg ctggagcaac ctgccagcag agtagaaccc aggattaccg
    21181 cgcccagtac cagtttagta gctttcatta ttaataccct ctagattgag ttaatctcca
    21241 tgtagcgtta caagtattac acaaagtttt ttatgttgag aatatttttt tgatgggaag
    21301 gcacttattt ttgatcgttc gctcaaagaa gcatcgaacg ctgggtttta gataaaaaaa
    21361 ttgagagaaa acaggatgct tccatcggat tcatcttaga taaagtgaga ttatatagtg
    21421 aaatccgatt tgatttttta ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca
    21481 gggcgcttcg atatacaaat taattcacaa aagcaatatt acaggacgtg aacagatgcg
    21541 gtgttagtag tgccgctcgg taccagtgca ccagaaacca taactacgac gtcacctttg
    21601 tgtgccagac cgctctgcag agccagttct ttacccagac ggtagaaatc atcagtagaa
    21661 gtgatctctt taaccagctg cggcacaacg cctttgctca gtaccaactg atgagccgtt
    21721 ttttcgttgg tggtcagtgc caggatggtg gcatccggga agtatttacg tactgcgcga
    21781 gcagatttac cgccctgggt agcaaccacg atcagcggag catccagttt ttcagcagtt
    21841 tcaacggcac cacggcatac cgcttcggta atgcgcagtt tacggttgtc attgttgaac
    21901 tcgagacggc tgttcatcac gcggtcggta cgttcgcaga tggtcgccat gatagaaacc
    21961 gcttccagcg ggtatttacc ttttgcggat tcaccagaca gcatcactgc gtcagtaccg
    22021 tcgaggatgg cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc
    22081 atggaatcca gcatctgggt cgcagtgata acgactttac gtgcacggat acatttttcg
    22141 atcatcatct tctgggcgaa gataacttct tctaccggga tttctacacc caggtcgcca
    22201 cgcgcaacca tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt gaggccttcc
    22261 tggttttcga ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt caggtgctca
    22321 cggatttcga taacgtcaga acgcttacga ataaaggaag cagcaacaaa gtctacgcct
    22381 tgttcgcaac caaagatcag gtcctgtttg tctttttcag ccagtgctgg cagagcaatg
    22441 gaaacgccag gcaggttcac acctttgttt tcgcccaggt caccgttgtt cagcacttta
    22501 cagataactt tgttaccttc aatggcggta acttccatac cgatcagacc atcgtcaacc
    22561 agtacggtgt tgccaacaga caggtcagta gtgaaacctt catacgttac cgcaaccatt
    22621 tcgctgttgc cgataacaga tttatcagtg gtgaaagtaa aggtctgacc agctttcaga
    22681 gaaacgtcgt taccgccttc cagtttcatg gtgcggattt ccggaccttt ggtatcaagc
    22741 aggatagcgg cggttttacc agttttgctc atcacgttgc gcagattctg aatgcgctga
    22801 ccgtgttctg catagtcacc atgagagaag ttcagacgca taacgttcat gccagcgtcc
    22861 agcattttag ctaacatctc ttcagattcg gttttcggtc cgatggtgca aacaattttg
    22921 gtctttttca tgacagtctt agtctttaag ttgagaagga tgggagaaac ttgctttctg
    22981 ggcgcactgc cggaaagcga atccggtatt acgaaagtgg tggtgcctcg ctctaaggat
    23041 aggtgacatc gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg
    23101 cctgtttttg agtcgttgag ttctacagtc caaagtgctg aaaccattca agagtcaatt
    23161 ggcgcgcatt atacgctgaa attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaaa
    23221 tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga aaaggttacg
    23281 caaaaacttc actgtttgcg caacgtctgg tgccgaagtt gtcagtgtga ggataagtga
    23341 aagttcatcc ggtgtataaa tatatcatcg ctcaattg
//
LOCUS       DAFNBJ010000071        23300 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000071 DAFNBJ010000000
VERSION     DAFNBJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.071"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            965..1756
                     /locus_tag="L2X49_002619"
     CDS             965..1756
                     /locus_tag="L2X49_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797690.1"
                     /translation="MTTRITFNMTSDETLRIVDEYCHTHKLSRSKVIDALLSATAPVL
                     NDINCYYQLAGKLQSRLLNGVYQRDLPHKRNVVSAEKYCLEIWENKLFTKRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKAKYACFIYIERTIYNHD
                     NPSGGTPVKAAVGNAVILLAKDVIYDEYFFDLRQSFFVSVKDLMASGAKGIPETQKYP
                     DVYCWIPLFSINSGVVITPVYKIDPRKPLTVKKPDQITVVCNYRE"
     gene            2141..2347
                     /locus_tag="L2X49_002620"
     CDS             2141..2347
                     /locus_tag="L2X49_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBN4797691.1"
                     /translation="MTPPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2441..3043
                     /locus_tag="L2X49_002621"
     CDS             2441..3043
                     /locus_tag="L2X49_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797692.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIHYFHTLT
                     PGWVSFNGEKPEIAIVPRSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDDSRN"
     gene            4243..5160
                     /locus_tag="L2X49_002622"
     CDS             4243..5160
                     /locus_tag="L2X49_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797693.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALASLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRTKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWIRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            5245..6117
                     /locus_tag="L2X49_002623"
     CDS             5245..6117
                     /locus_tag="L2X49_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBN4797694.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMIITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTDAVFRLFRPVHPVVAVSACTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARAVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            6489..9335
                     /gene="ag43"
                     /locus_tag="L2X49_002624"
     CDS             6489..9335
                     /gene="ag43"
                     /locus_tag="L2X49_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBN4797695.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRTGVAVALSLAA
                     VTSLPALAADTVVQAGETVNGGTLENHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVTGSNRLGNFTVE
                     NGNADGVVLESGGRLDVLEGHSAWKTLVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQASNTTVGHRGTLMLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIYFDNQTTPDAVLSRAVAKGNAPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSAK
                     HRVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQGGYAHSVSGSSAEGYNGQATLNVTF"
     gene            9406..9564
                     /locus_tag="L2X49_002625"
     CDS             9406..9564
                     /locus_tag="L2X49_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBN4797696.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            9720..10541
                     /locus_tag="L2X49_002626"
     CDS             9720..10541
                     /locus_tag="L2X49_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBN4797697.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            10883..11185
                     /locus_tag="L2X49_002627"
                     /pseudo
     CDS             10883..11185
                     /locus_tag="L2X49_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
     gene            complement(11183..12838)
                     /gene="paoC"
                     /locus_tag="L2X49_002628"
                     /pseudo
     CDS             complement(11183..12838)
                     /gene="paoC"
                     /locus_tag="L2X49_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
     gene            complement(12835..13791)
                     /gene="paoB"
                     /locus_tag="L2X49_002629"
     CDS             complement(12835..13791)
                     /gene="paoB"
                     /locus_tag="L2X49_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HBN4797698.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFAQVSVAAIIHPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            complement(13788..14477)
                     /gene="paoA"
                     /locus_tag="L2X49_002630"
     CDS             complement(13788..14477)
                     /gene="paoA"
                     /locus_tag="L2X49_002630"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HBN4797699.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            14895..15509
                     /locus_tag="L2X49_002631"
     CDS             14895..15509
                     /locus_tag="L2X49_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HBN4797700.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(15757..16086)
                     /locus_tag="L2X49_002632"
     CDS             complement(15757..16086)
                     /locus_tag="L2X49_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBN4797701.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(16399..17109)
                     /gene="ecpE"
                     /locus_tag="L2X49_002633"
     CDS             complement(16399..17109)
                     /gene="ecpE"
                     /locus_tag="L2X49_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HBN4797702.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            complement(17078..18721)
                     /gene="ecpD"
                     /locus_tag="L2X49_002634"
     CDS             complement(17078..18721)
                     /gene="ecpD"
                     /locus_tag="L2X49_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HBN4797703.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            complement(18711..21236)
                     /gene="ecpC"
                     /locus_tag="L2X49_002635"
                     /gene_synonym="yagX"
     CDS             complement(18711..21236)
                     /gene="ecpC"
                     /locus_tag="L2X49_002635"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HBN4797704.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            complement(21262..21930)
                     /gene="ecpB"
                     /locus_tag="L2X49_002636"
                     /gene_synonym="yagY"
     CDS             complement(21262..21930)
                     /gene="ecpB"
                     /locus_tag="L2X49_002636"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HBN4797705.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            complement(21988..22575)
                     /gene="ecpA"
                     /locus_tag="L2X49_002637"
                     /gene_synonym="matB"
     CDS             complement(21988..22575)
                     /gene="ecpA"
                     /locus_tag="L2X49_002637"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HBN4797706.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            complement(22650..23192)
                     /gene="ecpR"
                     /locus_tag="L2X49_002638"
                     /gene_synonym="matA"
     CDS             complement(22650..23192)
                     /gene="ecpR"
                     /locus_tag="L2X49_002638"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HBN4797707.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLSTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
BASE COUNT         5505 a         5962 c         6076 g         5757 t
ORIGIN      
        1 ttccacttcc ctgtttctcc ggtaaaagat gtttttaata ctgaaggcat aacgccataa
       61 ccagatatta caattaacgt agctttccgc aacgtactac atgagtaaca gaggtacagg
      121 gttaaacaac actccggaag actttttaat gacatgaggc atactctttt acgtattact
      181 accaaaactc atcaggtgag ctgcaggaac agatcattaa cagtaattcc ttaatcaccg
      241 ttatccacag tatcaacact ggcgaagtca tactcttagc tataaaggtg atgacgggtt
      301 cagaatagtt attacaccat acaacaaaat acagaaaaca acaggaaaaa taacagaaaa
      361 aaaatagcag acattactta acaaagcacg ttacagtcag tgtgtgtaca ttacagattt
      421 atattcaggt gttacgggat tacattgcag cagagagggt taaggacagt gttacaagta
      481 ctacagagaa tatcgctgca cagatacttc tatgagacag acttctgcag gacctggtta
      541 caaggtgtat ttacgaggta caattacagg ggagagttac aggatagaat tacagagttc
      601 atttacacgg tagaattaca gggtcattta catgaccata cttctataaa taatactaat
      661 taaccttaat tattttttaa tggtacatga actgcctgta ggtgtaacga taaacaaatc
      721 acccgaaacg aaaaatactg gttatcatgg ttcagagaac tcatacaagt ggcagctccc
      781 cccctgagca atgcttcatt ttcggtggtt ctgtgtgata gagttatact gactacagtg
      841 gcagtaacac tgctttccac cacactagga taaagccagt aaaaaaaatc aattatatga
      901 attaaaatca ttttttatga aatgttatgc atatcaacca gtatgattaa cagaaaattt
      961 catcatgaca acacgtataa ctttcaatat gaccagtgat gagacgctgc gaattgtgga
     1021 tgagtactgt catacccaca aactttcgcg ctctaaggta atcgacgcat tactcagcgc
     1081 cacagcacct gtccttaacg atattaactg ttattatcaa cttgcaggca aactgcagtc
     1141 acgcttatta aatggagtgt atcaacggga tcttcctcac aaacgcaatg tcgtcagtgc
     1201 agagaagtac tgtctggaga tctgggaaaa caaacttttc accaaaagaa tcctggagtt
     1261 tgacagttca aatggtgttc tgtacgcgct gaaacataaa agacactatc gccgggacaa
     1321 gatgattgga agggttgaaa gccggtgtat caaagacatt tgcgaatacc agatgcaact
     1381 gtccggggag aaggcaaaat atgcctgttt tatttatatc gaaagaacaa tatacaatca
     1441 cgataatcca tccggtggga cacctgtaaa agcggctgtt ggtaatgcag tcatcctgct
     1501 ggcaaaagac gtgatatatg acgagtactt ttttgatctg cggcagtcat ttttcgtgag
     1561 cgttaaagat ctgatggcct ctggcgcaaa aggtattccg gagacacaga aatatccgga
     1621 cgtttattgc tggatcccgc ttttctccat aaattccggc gtggtcatta ccccggtcta
     1681 taaaattgat ccccggaaac ccttaacggt aaaaaaaccg gatcagatca ctgttgtttg
     1741 taattacaga gaataatttc agtcttctcc gttcaccgca acagtgttta cctctgcttt
     1801 atcttccgtt cagtaaatac aaaccaccgc tctggcaggt acagggcgtg gtttatctcc
     1861 gatactcccc tgcgcagtaa taatcatcac tgaaaatact gatcccgtca cggcaaaacg
     1921 ccatcccata ttccggcaac acaaaatcat cttccgttta taccggcttc actgacaaaa
     1981 cagacaacct gtattgcctg tacacaacag tttctctcct ggcaggaata ttcccctact
     2041 gattttttac gtttctcccc ggttgttatg ccggtgaagg tggtgcgttg ttttcatcat
     2101 caacgccggt tgagattaac aacatccgga ggagattttt atgacccccc cagtttcgct
     2161 gatggatgac cagatggtcg atatggcgtt tatcactcag ctgaccggcc tgaccgataa
     2221 gtggttttac aagctcatca aggatggggc ctttcctgcg cccatcaaaa tggggcgcag
     2281 ctcccgctgg ctgaaaagtg aagtggaagc ctggctgcag gcgcgtattg cacagtcccg
     2341 tccgtaattt ttaaccactg gcccttcgcc cacagcagac ataatctccg gcctgccggc
     2401 ggcactctgc tgcctgttat cccccgtgag gaatatgaaa atgaaaccac agtatcagac
     2461 ccgttacgaa ctgctccacg aaagctacca gaaatggctg accggcttca cccgacatgc
     2521 cgtatcctgg ggcgtgtgtc atccgaatat ccactacttc catactctga cgcccgggtg
     2581 ggtgtcattc aatggtgaaa aaccggagat tgccatcgtt ccccgtagtc tgcaccggct
     2641 gatttatggt ccggacaaac ggagttcccc gtctctggat gatgatctgg tggtgaactt
     2701 atgcaccagt gaacatctgc tggttcatca tccgatgctg gaaggcattc tgctgtctga
     2761 atgtgaacgt ctgaagcaac attctctggc aaacaaactg atcagcctct tccgtcagtt
     2821 tggcggcacg gagttacgtc tcaaactggt ctggctctgc tggcttgact taatgaccgg
     2881 aaacagcctt gacgactgga cgaaaaacct gaaacacaaa tcagaaaaag atctggagca
     2941 atggatcatt gcacgtcagg gtcagagtga accactgaca aatctgatgg atcagtacgt
     3001 gcttatggca taccgtacat cggttgacga tagccgcaac tgatgccggt tgcttccgga
     3061 aggtgtgaca aaatacggaa atgatgatgc tcccggcccc catgctcacc tgcagagcgt
     3121 gttcgtcgtc atcatactga gccatgacgg gcgtcttaag ggcatcctgt cattgctgag
     3181 caggcaacac caccagagga gccacggaca gggatgttct ttacagcccg atacacgcca
     3241 ggttctcagc agactgaatg tgcaggtgcg gttattcacc agaaatggca tgctgcagta
     3301 catacccatc ctggtgacaa tatgtacgtg gcgacagcga tcccggcact gtggaagaga
     3361 ctcactcagc ctgcaggata ttctttccgg catccgggaa tgtccgcctg aatgcaaaca
     3421 gggtgatatt tgaatccgta atactgttct ttcatcataa ataatttatg cagatacagt
     3481 taaccgttac agtagccggt ttgttttacc gggaaataat atccgtcaga ctgatgccgg
     3541 aacatatctg aaacacgtta tgatgttttg ttatcagaaa tgttgtatat tcttatccgt
     3601 ggcgacagtt taatgccctc tggtatcacc tgataatgca tatcctgcga tgattcgtta
     3661 tatttacggg acacgcctct tttcattgtg ctccttttca gacttcattt tccgtaagtg
     3721 ttccccggca taaaggaggt acctttctgg cgtttataac tctctttatc ggtgacgccg
     3781 gtaatacagg gaataccaca ccgacaaacg aggtattaac agacgatatt tcagtgtcca
     3841 taatttttct gtccggttaa ataacgtcag tctgctgccc tctgactgta atcatcaaag
     3901 ccggaatgca taccgggtta caacgtaaaa aaagtgatgc tggcccccgg cgcagctggc
     3961 gggcgaaaca tgtttacctt tcaatacccc ctgtctgcag tctccactct tccattctct
     4021 taagctacgt cggccacatg agtgcacttc catgataaca ggtattcctg tcagcataac
     4081 gaagccggtt accttccctt cctcctgcat ttcctgtcag aatgactata cagcgcgcag
     4141 attgcctttt tattgaccat cttaatatac cattataaac ttctttttat catgttatta
     4201 tcaccttgtt gtgtttaatt tttaagcaga cggagaaaga ggatggttca gaaaattttg
     4261 tcggataagg ttatgaatga aagaacaaat gcgtattact cttattatct tggagagcgt
     4321 aatatctccg tactgcccct taatgtttat gatcccccgg aacgttttat cgcttacata
     4381 aaaaaaaaca gagaaaatct gaacatcaca ctttcagatt ttgaacttga acaaatcatt
     4441 tctggtatgc gtctgaaggc actggcctcc ctggtgcccc tggaaaaaat atcctggatc
     4501 gcgggcagtg aacgcgcatg cttattttcc tggtatctgt tgatgcagtt tatccagaat
     4561 aacaggacaa aaataagcgc agacttgctt caaaagaata aactgtatct taaagaagag
     4621 tatttagaag ggaatgcctt cccgtcggac tcatcgacac agttcagaca aatactcaga
     4681 gttctcgata ttctttctga taagaatttg cgggatgagt ggataatcca gaccaaagac
     4741 agatggatac gggcatttaa atcaaagagt cctttcagtt acctcctccc ggagaacgaa
     4801 catgaatgca tatggacatg gaattatttg aagggaaaaa atattgcact ggaaaaactg
     4861 gccagttttc ctggttctgc tgatatttac cacgcaattc acctttcatt tgacatatgg
     4921 gttacctgtc cactcacctc acctgacgat ataaaaaatt tcagaaacag tttcaataag
     4981 gccaaggcgc agcgcaagta caaaaaaatg caggaagata aagtcaatgt acagtttttt
     5041 cttgacgcag aaaccagggc gcagctcaaa gaattatcga gggtcagaag actcagtacc
     5101 ggcgaaatgc tgcatgacct gattgttgaa gagtataaaa ggtaccgaca ttccagataa
     5161 tttcagacat atattatctc cgtgagtccc ccgccacctt tccggtgtgc gggggttttg
     5221 tcttttttca ccgggaatac atgtatgaat ccgtctgatg ccattgaggc aattgaaaaa
     5281 ccgctctcct ccctgcctta ctcactttcc cgtcacatcc tggaacatct gcgcaaactc
     5341 accagtcacg aacccgtgat tggcattatg ggtaaaagcg gggccggtaa atcctcactc
     5401 tgtaatgcac tgtttcaggg ggaggtcacc ccggtcagtg atgttcacgc cggcacccgg
     5461 gaagtgcggc gcttccgtct gagtggccac ggtcacagca tgattatcac tgacctgccc
     5521 ggggtgggcg agagccggga cagggatgca gagtatgaag ccctgtaccg tgacattctg
     5581 cctgaacttg acctggtact gtggctgatt aaagccgatg accgtgccct gtctgtggat
     5641 gagtatttct ggcgacacat cctgcaccgg ggacatcagc aggtgctgtt tgtggtgacg
     5701 caggccgaca aaacggagcc ctgccatgaa tgggatatgg ccggcattca gccttctccc
     5761 gcacaggcac agaacattcg tgagaaaaca gatgcggtat tccggctgtt ccggcccgta
     5821 catccggttg tggccgtatc ggcctgcacc ggctgggaac tggatacgct ggtcagtgcg
     5881 ctcatgacag cgcttcccga ccatgccgcc agtcccctga tgacccgcct gcaggacgag
     5941 ctgcgcacgg aatctgtccg gtctcaggcc cgtgaacagt ttaccggtgc ggtggaccgg
     6001 atatttgaca ctgcggagag cgtctgtatt gcctctgttg cacgcgcggt tctgcgtgcc
     6061 gtccgtgact cggtggtctc tgttgcccgc gctgtatgga actggatttt cttctgaaca
     6121 ctctgccagg ggaaatgcct gattacattg cgttaaccaa ctgaaaacat gacacaaaca
     6181 cctcagaccc acctctttct cttgcgtgac tgccttgctg ttaatagaat aaaacgatcg
     6241 ataaaacagg tatcgatcgt tactatcgat cgtttatagt ggttaatggt ctgcgaaaat
     6301 gcagctgaat ctcttcatca tgcagaacgg aattgcacac aacggactga tacttctctg
     6361 gcggatgaga gaggggaagc atttatgtcc gggaaaaacc actttatctg accatgctgt
     6421 tttgtacctg ccggtatcca catttgtggg taccggcttt tttattcacc ctctgtaagg
     6481 aaaagctgat gaaacgacat ctgaacacca gctacaggct ggtatggaat cacattacgg
     6541 gcaccctggt ggtggcttcc gaactggcgc gctcacgggg aaaacgcacc ggtgtggcag
     6601 ttgctctgtc tcttgctgct gtcacgtcac tcccggcact ggctgctgac acggttgtcc
     6661 aggcgggaga aaccgtgaac ggtggaacac tggaaaatca tgacaaccag attgtcttcg
     6721 gtacggccaa cggaatgacc atcagtaccg gtctggagta tgggccggat aacgaggcca
     6781 ataccggcgg acaatggata caaaatggcg gtatcgccaa caacactact gtcaccggtg
     6841 gtggtcttca gagagtgaat gccggaggaa gcgtttcaga cacggttatc agtgccggag
     6901 gcggacagag ccttcagggg caggcagtga acaccactct gaacggcggt gagcagtggg
     6961 tacatgaagg cgggattgca acgggtaccg tcattaatga gaagggctgg caggccgtca
     7021 aatccggcgc aatggcaacc gacacggttg tgaataccgg cgcggaaggg ggaccggatg
     7081 cagaaaatgg tgataccggg cagtttgttc gcggaaatgc cgtacgtacc actatcaatg
     7141 aaaatggtcg tcagattgtg gctgctgaag gaacagcaaa taccactgtg gtttatgccg
     7201 gcggcgacca gacggtacac gggcatgcgc tggataccac actgaatggc ggttaccagt
     7261 atgtgcacaa cggaggcaca gcctctgaca cggttgtaaa cagtgacggc tggcagattg
     7321 tcaaggaagg tggtctggcg gatttcacca ccgttaacca gaaaggcaaa ctgcaggtga
     7381 acgccggtgg tacagccacg aatgtcaccc tgaagcaggg aggcgcactg gtcaccagta
     7441 cggcggcaac cgtcaccggc agcaaccgtc tgggcaattt cactgtggaa aacggtaatg
     7501 ctgacggtgt tgttctggag tccggtggtc gcctggatgt actggagggc cattcagcct
     7561 ggaaaacact ggtggatgac ggcggaaccc tggcagtgtc tgccggtggt aaggcaacag
     7621 gtgtcaccat gacatccggt ggtgccctga ttgcagacag tggtgccact gttgagggga
     7681 ccaatgccag cggtaagttc agtattgatg gcatatccgg tcaggccagc ggcctgctgc
     7741 tggaaaatgg cggcagcttt acggttaatg ccgggggaca ggccagcaac accactgtcg
     7801 gacatcgtgg aacactgatg ctggctgccg ggggaagtct gagtggcaga acacagctca
     7861 gtaaaggcgc cagcatggta ctgaatggtg atgtggtcag taccggcgat attgttaacg
     7921 caggggagat ttactttgac aatcagacaa caccggatgc cgtactgagc cgtgctgttg
     7981 caaaaggcaa cgccccggta acgttccata aactgacaac cagtaacctc accggtcagg
     8041 gtggcactat caatatgcgt gttcgccttg atggcagcaa tgcctctgac cagctggtga
     8101 ttaatggtgg tcaggcaacc ggcaaaacct ggcttgcgtt tacaaatgtc ggaaacagta
     8161 acctcggggt ggcaaccacc ggacagggta tccgggttgt ggatgcacag aatggcgcta
     8221 ccacagaaga aggtgcgttt gccctgagtc gcccgcttca ggccggcgcc tttaactaca
     8281 ccctgaaccg tgacagcgat gaagactggt acctgcgcag tgaaaatgct tatcgtgctg
     8341 aagtccccct gtatacatcc atgctgacac aggcaatgga ctatgaccgg attctggcag
     8401 gctcccgcag ccatcagacc ggtgtaaacg gtgaaaataa cagcgtccgt ctcagcattc
     8461 agggcggtca tctcggtcac gataacaacg gcggtattgc ccgtggagcc acgccggaaa
     8521 gcagcggcag ctatggcttc gtccgtctgg agggtgacct gctgagaaca gaggttgccg
     8581 gtatgtctct gacgacaggg gtgtatggtg ccgcaggcca ttcttccgtt gatgttaagg
     8641 atgatgacgg ttcccgcgcc ggcacggtcc gggatgatgc cggcagcctg ggcggatacc
     8701 tgaatctgac acacacgtcc tccggactgt gggctgacat tgtggcacag ggaacccgcc
     8761 acagcatgaa agcgtcatcg gacaataacg acttccgcgc ccggggctgg ggctggctgg
     8821 gctcactgga aaccggtctg cccttcagta tcactgacaa cctgatgctg gagccacaac
     8881 tgcagtatac ctggcaggga ctttccctgg atgacggcca ggataacgcc ggttatgtga
     8941 agtttgggca tggcagtgca caacatgtgc gtgccggttt ccgtctgggc agccacaacg
     9001 atatgagctt tggtgaaggc acctcatccc gtgacaccct gcgcgacagt gcaaaacacc
     9061 gtgtgcgtga actgccggtg aactggtggg tacagccttc tgttatccgc accttcagct
     9121 cccggggtga catgagcatg ggtacagccg ccgccggcag taacatgacg ttctcaccgt
     9181 cccggaatgg cacgtcactg gacctgcagg ccggactgga agcccgtgtc cgggaaaata
     9241 tcaccctggg cgttcagggc ggttatgccc acagcgtcag cggcagcagc gctgaaggtt
     9301 ataacggtca ggccacgctg aatgtgactt tctgataatc aggtcactct ccttcaggcc
     9361 ccggtcatca tgaccggggc tttttacttt cagggagcaa cagatatgcc gggttgtaca
     9421 tcacgtttat tgcccgaagg ccctttctca cgcgaacagg ctgttgccgt caaaacagct
     9481 taccgcaatg tgtttactga agatgaccag ggaacatatt cccggctggt tatccgtaat
     9541 gctgaaggtc agttgcgctg gtgatgccgg aacacagctt aatactcagc tcgccagtta
     9601 agggcttctc agacaataaa cgtcattcat ttcatctgtc aggccgcgtc ttctccggga
     9661 gacgcggctt ttttcattta ttacaccagt aaatcttaac caccgataag gagcaaagta
     9721 tgcgattagc cagtcgtttt ggttatgctg caaaccagat acgccgtgac cgtccactga
     9781 cacatgaaga gctgatgcac catgtgccgg ggattttcgg ggaagataaa cacacgtccc
     9841 gaagccagaa ctatacgtac atccccacca tcaccgtgct ggaaagtctg cagcgggaag
     9901 gcttccagcc attcttcgcc tgccagaccc gtgtgcgcga cccgggccgt cggggataca
     9961 caaaacacat gctgcgtctg cggcgggccg gagagataaa cggagaacat gtccctgaaa
    10021 ttattctgct caactctcat gacggtacct ccagctacca gatgctgccg ggttacttca
    10081 ggttcgtctg ccagaacggg tgcgtctgtg gtcagtctct gggggaagtg cgtgttccgc
    10141 accggggaaa tgtagtggag aaagttatcg aaggggctta cgaggtggtg ggggtttttg
    10201 accggataga ggagaagcgt gatgccatgc agtcgctggt cctgccgcca ccggcacgcc
    10261 aggcgctggc tcaggcggca ctgacttacc gttatggtga cgaacatcag cccgtcacca
    10321 ccgccgacat tctgacgcca cgacgccggg aggattacgg aaaggacctg tggagtgctt
    10381 atcagaccat ccaggagaat atgctgaaag gggggatttc cggtcgcagt gccagaggaa
    10441 aacgtatcca cacccgtgcc attcacagca tcgacaccga tattaagctc aaccgcgcat
    10501 tgtgggtaat ggcagaaacg ctgctggaga gcctgcgctg atgcagcatg tccctgaagg
    10561 cgtgattcag gcaccagaat cacccgacgc cccggaggcg ctgccggggt gtcgttccat
    10621 ttctgatgat gacctgtgcg cctggtgtac ccgtctgctt taccgtcccg gagaaatcag
    10681 tctgtgcaga ctgagtatac aggcaatggc atctggccat ccgtctgtga ccggaacggt
    10741 tacgcgtacg actgtccgga atttcagccg aatatcattc atccctgata ttaccgggtc
    10801 atcacggtca gtgatgaacg tgatgttgcc agctcccgac ctttactccc tctcatttat
    10861 tcacattaca aggatttcat acatgaaaac attgtctcag aacaccacct cttcggcctg
    10921 tgcaccggag actgacctgc aacaactggt tgccaccctc gtccctgatg aacagcgcat
    10981 cagcttctgg ccgcagcact ttggcctcat tccacagtgg gtgaccctgg agccccgtgt
    11041 cttcggctgg atggaccgtc tgtgcgaaga ctactgcggt ggtatctgga atctgtacac
    11101 cctgaacaac ggcggggcat ttatggcacc cgaaccggat gacgatgatg acgaaacatg
    11161 ggtactgttc aatgccatga acggtaccgt tggtaatctt gccgtcggca aactgcgact
    11221 gctcaggatc aaacccgact gccgaggcaa tcatttcgcg aagcttcaca caggcggcgt
    11281 aaacgccgga ggtggaggta ttcgcgcccc attgtccacc agaacccgca gaaaccggga
    11341 aactggaatc gccgaggtga accgcaacct gctccagcgg tacgccaagc atttccgctg
    11401 ccgtctgggc cagaatggtg tagctgccgg tgccaatgtc ggtcatgtcc gtttccacgg
    11461 taacggtgcc gtttggttcg aggtgaaccc gagcccccga tttttccagc agattattgc
    11521 gaaagcccgc cgcgacgccg tggccgacta gccactcccc gtcgcgcacc tgtccgggtg
    11581 tggcgttgcg ctgcttccag ccaaatttat ccgctccggt gcgcaagcac tcgataagct
    11641 gacggcgaga gaagcggcgc gtcgggtcgg cggggtcaac ctgagtgtca ttcaggatgc
    11701 gaaactcgac gggatcgatg cccgcttttt ccgccagttc gtcgatcgcg atttcgagcg
    11761 ccatcagacc gggggcttcg ccgggcgcac gcatggcgtt cccttccggc aaatcaagcg
    11821 tggcgagccg caggccggta tgacggtttg ccccggcgta gagtaattcg ctttgctgta
    11881 ccgccgtttc cggcgtgccg ccgggcaggt ttccggacca gctttcatgt gagatagcgg
    11941 tgattttccc gctctggtca gcaccaatac gcaggtgctg aagggttgcg gggcggtgcg
    12001 tggtgttatt gggaatagtg gggcggggga gcatcacttt aaccggacgt ttcaccgctc
    12061 gggcggcgag ggctgccagc agcgcatcgc ttctcaggaa cagcttgccg ccaaaccctc
    12121 cgccgatata cggggagata atacgcacat tctccacggg cactttcagc gtttttgcca
    12181 gatcggtgcg gcaccagtca atcatctgat ttgaggtcca gagagtaagc ttatttccat
    12241 cccagacggc catcgaggca tgcggctcca tcgccatatg gctctggtcc ggggtcgtgt
    12301 aggtagcatc aatcttcacc gcagcggagg agaaagcccc gtcaaagtca ccgacgtttt
    12361 tgtcgggcgt gccttccggc ggctgattga cggcctgttt ttcgtccgcc agggagtaag
    12421 ctcctttatt acggcgatag tgcgcctgca ccagcgaggc cgccgctcgc gcctgttcga
    12481 aggtctcggc cactaccagc gcaatggcct gatgatagtg ctcaatagtg gggccgccta
    12541 acagcctggc ggtgtttttg tcgcctttgc cgagtgcccc ggcgttactg gcggtaatga
    12601 cagccagtac gcccggcgct ttttgcgcgg cgtccgtatc aagggcggtg aggcgtcctt
    12661 tggcaatggc ggaaccgacg atatagccat aggcggcgtt gggggcctct tcatgccatt
    12721 cgtaggcgta gcgtgccgtg ccggtagttt tcagcggtcc gtcgatgcgg tcatggggac
    12781 gaccgacaac cttcagctga tcgatcgggt tttcccctgc gggtttatca aatttcatgc
    12841 ctgtgccctc gcttcagcca gtacggaggc aagcgttcgc ttcgccaaca ggagtttaaa
    12901 ggtgttttca gcggtgggat gggcgctggc gaacagcgtg tcatatacgg cctgcgcccc
    12961 ctgggatagc tgagcatccg cagcctcaat gcgccagggc ttatgtgcta ctccgcccag
    13021 cgcgacgcgc ccgctgccgt cagggtgaat aatcgccgcg accgatacct gggcaaaggc
    13081 gtaggaggcg cgatcgcgca ccttacggta gatatgtttt ccgccgagcg gcggaggtaa
    13141 cgtcaccgca acgataagct caccgggaag cagggcggtt tcaatgtgcg gcgtttttcc
    13201 cggagggtga taaaaatcag ccagtgtgat actgcgagtc tttccctccg gcgtgatggt
    13261 ttccaccacc gcatccagca accgcattgc gaccgccata tcgctcggat gggtggcaat
    13321 gcaggcttcg cttacgccta ccaccgcgtg ctgacggcta aagccttcaa gcgccgcgca
    13381 gccgctcccg ggcaggcgct tattgcaggg ctgattggtg tcgtaaaaat aggggcagcg
    13441 cgtgcgctgg agcagattac ctgcggtggt tgcctgatta cgtaactgac cagatgcgcc
    13501 agcgagcagg gcgcgggaga gtaccgcgta atcacgacgc acgcgctcgt gagccgccag
    13561 gtcggtgttc cgtaccagtg cgccgatgcg cagcccaccc gcgtcggtca cttcaatctt
    13621 atcgagcccg aggccgttca catcgataag gtgggtgggc gtttcaattt ccagcttcat
    13681 caggtccagc agattggtcc cgcccgcgat aaattttgcg ccgggtacgc gctgagcgct
    13741 aagtgccgcc tcggctgggg tgttcactcg ttcataggta aacgccttca tgattttatc
    13801 tcccccgcag catcttcaat ggcggcaagg atgttagcgt atgcaccaca gcgacagatg
    13861 ttgccgctca tacgttcacg gatctcatcg gcagttgttt ctggagcgga aaccaaatcg
    13921 accgtgacgt gactgggaat gccgtcctga atctctttta gcaccgctac tgatgagcaa
    13981 atttgcccgg aggtgcagta gccgcactgg aagccatcat gcttgataaa ggccgcctgc
    14041 atggggtgaa gattatctgg cgagcccagg ccttcaatgg tggtgatctc ggccccctga
    14101 tgcatgactg caagcgtcag gcaggcatta agcctgcgac cattgaccag cacggtacag
    14161 gctccgcact gtccgtgatc gcaacctttc ttggtaccga tcaaatgcag attttcacgc
    14221 aaagcgtcca gtagcgtggt tcgggtatcc acctcaagct gctcggtttt gccgttcacc
    14281 ttcagtgtca ggggcattat ctctggcgcg ggtgtagctg ccggaacgct tgccgccagc
    14341 gtagaatgag gataaaccac ggcggtcgcc gctgttgcgg cgctcacttt aatcagatcg
    14401 cgacgggtca aacttaaatc gtgcggctcg tgcttcccaa cccgattatc ttcggggtat
    14461 tcgccttggt tgctcatacc aggcctccgg tattctaaag gggaaaataa gagtgcgttg
    14521 cgagagtgct gatgcttttt taaatgttaa gcatagtcgg cgacagcagg aggattattc
    14581 ttaaaacgcg aatgcagcta tgagcccggc taataaattc atgttcgccg ggatgttgat
    14641 gatgatggga gctggtttat tgctttagtt gtacgatgca aaaaccaata aggaaacctg
    14701 tgattttcag ctctgcatca ccctgcaaat ctctgtcact tttaatataa aaatagggag
    14761 aaatgatgga gcttatattc attggcgatt aggaaaccat cttgttatac aaaacaatac
    14821 tgttctttac atttgccttg ttttatgaat actcctgaag aggtgtataa cataatggta
    14881 caagcagggt agatatgaat atatttgaac aaactccacc gaaccgcaga cgttatggtc
    14941 ttgctgcatt cattgggctg attgctggcg ttgtttccgc attcgtgaag tggggggctg
    15001 aagttccatt gccgccacgt agcccggtgg atatgtttaa tgcagcgtgt ggcccggaat
    15061 cattaatcag ggctgcaggc caaattgatt gctcgcgtaa ttttctcaat ccaccgtata
    15121 tttttcttcg agactggttg gggctgacag atcccaatgc ggctgtttat acctttgccg
    15181 ggcatgtctt taactgggtt ggtgttacgc acattatctt ttcgatagtg tttgctgtcg
    15241 gttattgtgt ggtcgctgaa gtatttccaa aaattaaact ctggcagggc ttactggcag
    15301 gtgctttagc ccaacttttt gttcatatga tttcattccc tctcatggga ctgacgccac
    15361 ctctgtttga tctcccgtgg tatgagaatg tttctgaaat ttttggacat ttagtctggt
    15421 tctggtctat tgaaattatt cgcagagatt tacgaaacag aattactcat gagccagacc
    15481 ctgagatccc tttaggctca aacagataat gcattgaatg ataaaaatgg cgcaaataca
    15541 gcgccatttt tataggttaa aaacattgct ttttatattc tgatgcagat agtcagtgag
    15601 tatatcgcgc tacttcagga tgatgtagat ccgaagaacg ctacagaaga gaggcattgt
    15661 tgctggcaaa tagaagaagt atcgggtttt gttacccctg aaaaacgaag ccccgctatt
    15721 atcgctggcg gggcagtgca attaattatt gtatgtttat aacggtgtca gcccgtattt
    15781 agcccttgca cgattgcaag cctcattgac gactccgttc tcaccagaca tggcgaattc
    15841 tctgcatgtg gacgatcgat ttttatatat cgtgcagcag gcatttttgc ccggggttcc
    15901 tgcaagggca atacatcggg ggtttttctg attggtaccg ctcatgcatc ggtgaaaagg
    15961 ggatatttgt tcagtgagcc tggcgggaat agtaccgcca gcatcgtcgg cttcggccca
    16021 gtaaaaagag acgcggaaaa atgcacaaca ggcaccacac gtcatgcatg gattcagatt
    16081 gctcataatt caccagtaca gctaaaaatc gtaaagagaa cagcggtaca tcgtatgtaa
    16141 gaacgtaccg cggttggctg gggaactttc gatagtgtga gtattgaatg atttccagcc
    16201 gttaccgatt ttacgtacta attcgtgatt aaaccactcg ttagcaggct gcctgatacc
    16261 tttccgtgtc tcctgaacac tatggtgtgc atatgagaaa gcccatcgtt acaggtgaca
    16321 ttgacgatcg cttccacatc atttaaacga tttttggagc tctgaggtgg aattcttccc
    16381 tcgaccgatg gataagtctt agtccgccga agggggctta gccggacagg aatcgctaat
    16441 cttaatgaat ttgtcgttat agaccagata atgattcccc ggctgacgta actcaggctg
    16501 atgaaccacg tctcccggac gtagatacca ggcatcgcct tcctcttcgg tcgaatcaca
    16561 tcctggctta atcagtagct taaaccatgt gttgccagtg ttacttaccg ttcctgtcac
    16621 cttgtcgaag gaccatttaa actgaacctg acgtggtcgt actaccagaa tggtatccat
    16681 caccaccacc ggctccgtgc tgacctcgcc gccggttggg ctacgtcttg tctggttacg
    16741 agtggggact tcgcgaaatg agacccggta gtagcgctcg cggttatccc gtggaccatg
    16801 atagtaaaat ttaaaatact cgctctcacc agcctgcaac gccagttggc ggggggcgaa
    16861 aagcagttca ccatccaccg gtcgggtgcg caattcactg ctgcccgggc tatcaatagc
    16921 actgatggca atacggtata tccgtgcgct tttgttgtta ttcactacac gtttgctgac
    16981 aaagtcagtc tcggacggca gcgaaaatgt cagattgccg accgaaatcg cctgtgtcaa
    17041 aggtgccgcc agcatacaga ccgctgtcag cagggcctta gttaatgtta cgccacgtcg
    17101 cctgaacatg aatctctcct gaagcgctga cttcgccaaa ccagccgtta tcatctaccg
    17161 tacgcagcga aatggcgtta tccattggaa tcgaaaattt gactgtggtc ttatccatct
    17221 gccccacttc gccagagata tcagtccacg gtgtggtcaa ccacagcgca tcggtcatat
    17281 cccgccagct atcatcgcac ccggcatcgt aggtttttgt agctccgttg cgggtaataa
    17341 aggaaagtgt cgccgggaac ggtactttcg ccttaccgtc atcggagctg aagacacaat
    17401 aggagcgccc gccaatcacc tgcgcgggtc cggtcacctt gatcagcact tcgtcggcag
    17461 ctgttttacc gctggtggtt acgatatagc cgaaatcgag tgccgactcg ccgctgccaa
    17521 catatccctc ccgcgacggt gtctgagtat attcatcgga gataatactg atgctgaaat
    17581 cacggggttt gataatcagc gtgttggagg tagaaaattc ataccagcca gactccggtg
    17641 atgtggtgtt ctgaaagcga aagttgaata gatctttggt gttgatatcg ctgatcccga
    17701 ggttcaccat ctgcttaaag aagttgctcg agaagaaaac atagatcgag tctgcgctct
    17761 tcatctcgtt gaaggtgtag taataggcgg tattgctcac cggtttccat gaactgccat
    17821 tcagactgaa ctgcatatcg tacgccccga cggccgaggc taacgacgag ttggcgatcg
    17881 ccgggaatat atggattgag gtgttgctga gtccgtttgt ttgcagggta tagttgacca
    17941 tcttacaact taatcctgaa aggctgccga tggtctgtgt ccggcagtcg gcgttccctt
    18001 cgcccagagt cggtactccg tcgctgttga taaatacctc cgccagcgag tgggtattta
    18061 ttaaccgcag atttgctgct ttcgtatggg tgacgttgcg aacataccag ttgccggagg
    18121 cctgatcctt acagcgtgcg ccgctgctgg cgtcatagtt cacagaggtc tggcaggcat
    18181 tgatggtcat tgtaaagctg ctgccgacgg gcatttgttg cagatactgg taaaacgcgt
    18241 ctgacaacat gccgtgcatc cacttcgcgc cgccgctggt caccgtcgcg ccataaaatc
    18301 cactggcgtc ggtggtttgc ggcaggataa gactggtggt catattacac ccagcgtacc
    18361 agttgatgca gcgcaagcca gttaaaggag atgaaactgg tgaattttcc agccacatat
    18421 caaacttcca gttggtataa aggccggtgt tgtaaccgtt atcaatgtag ccgaggcttt
    18481 gctgatagat ggttcctgaa ccagtgtatt ttaaaccggt ccagcggttg gcaccggtca
    18541 ggcgcggatc cagcgccccg ccaggagtga cgaaaaagtt gtcgtctgag ttgttttcga
    18601 caaacacaaa ctcgcgtgcc ggtgcatccg cccaggttgt tttgctcact gccgctctga
    18661 gctcagttac tggccagatt agcgcgaaaa ttatcatcgt tattagtagg ttaactctca
    18721 ttctcttctc ctgtctgcgt caccgccgcc cacgatgaga ggccgctgca gaccacatca
    18781 ccgacccaga cggcaccgcg cgcctggttg agttccagtg ccacttcgca ggttttattg
    18841 ccactgtagc gaaaatcgat agtgggatat ttcttatcca cgtccatgac aaactcgccg
    18901 ttttcatcgg ttcgggttcg gccgatatgg ttgttaatcc gtgcgttagc cagcagtgtg
    18961 ccgtcttccg cacggatacg accggagacg gtaaccatct gcttcacctc tggctcaatg
    19021 acagcgacat tgcctggata gagagtcaga tggcttttgc ggccgctgac gatatcgtag
    19081 ctgtcgagtg agtttttgct gttctgtaac tccacctcat atcttccata gggagagagc
    19141 gggagatagt tacgcttgcc gttaagcggg aaaatccgcc cgttgatttt ggcgctgatc
    19201 tgaccgtcgt cctccagccc ggtgttgaat atcaccccag cgttgccatc agtccgcccg
    19261 ctggcagcga tgtttttacc ctgccagccg acgctgccat tggcggtcaa gttggtattg
    19321 atgtagccgt ccgccgcgct attgacgttc agcgttccgc tggcgtaacg agcgtcgaac
    19381 tgtgcatacg ccccaccgct gagggttttg tcatcaccgg tatcgccgga gatggcacgt
    19441 gacagattgg caccaacagt gcgaatggtt ccttcatcaa actgtttgcg tgctgacagg
    19501 tttgccatgg tgtagccgtt ttgatgggtc atccctgcgc taaaccagtt gcccagtggt
    19561 agcgagagat cgagagcgat atatttccct gtattggcgt tgctgtcgcc gttgttatag
    19621 cgctgaatac cggcccgcag gccaagcgaa ccaaaggtac cgctgtagac attttgatag
    19681 taatctgccg tgtaataatg gctgttgtaa cggcggtcat cattgtagct gatgctgaat
    19741 gtgcccagct tcgaccacag tgagttcagg ttgagtgtgc cgccgattgc acggttatcg
    19801 gcatcgctac gtcgcaattg attgccaatg cgggtttttt cctgattaac ccacagcgaa
    19861 ctaaagcctc ccggtagagt ggcgctgatg ctgccgatgc tgctccatga gctgtcactg
    19921 gccagcatat tttgcaggtt aacgttgatc gcccccccaa gcggcagcgt cagacgggtt
    19981 tcacccaccg cctgattatc gtatccatat cccgttgccg cccagctaag cgtactcaat
    20041 gagccggagg tcgaggcacc tgctagccaa ctctctttag ctggtcgcgt ctttttcccg
    20101 ttttccgacc agcgatccat atgaaagcta ccgccccata cctgccacgc cagtggtgca
    20161 ccgacgccgc gcccccggct aaacagctta ttgacccgct gggtgcgttt gctgatcacg
    20221 cgaccgttaa cgatcacctc aacttccaca tcgtaaatcc cgtacggtag accccgggta
    20281 tccacttcat gattgcccat agtgaagttc tgaacgctta gtagccgccc atcacgggtg
    20341 agatgtactt cgcccgccgc cggtaaaaag gcgatcactg gcgtggctga ctggctgctg
    20401 tcgaagatgg tggagctggc ctggtttccc caggaaaggc cgtaaatctt ccctgctgaa
    20461 atggcggtca tcggccctaa ggactgcaag ttccaggtgt cgagcattcc accggcaaat
    20521 cggtgaccgg caaaatcgcg ttcatacatc gctttatata attcactgtc ctgttgaccg
    20581 ctaccgatcc cgtacagcga gccgtcgagc accacatgat gttcgcgcag cgcagtaacg
    20641 ttattcagcg acagatagct ggatgtgttg ctcccgccgt tacgcaactg gttgttatag
    20701 acgcccaagt tatagctcag attactgctg agggtgttaa cactggactg cccgatgtct
    20761 tcgctacgtg agcgtagtac ggtgcccagc gcttcgcgct tgaccaccag ctgcagcagc
    20821 agttggcgca agctgagatc cagctgcgcg ttgtcagtca gggggatggt aagggcctca
    20881 ttgaacgggg cgttcgccag agccatcagc tgctgtcgag tttgttcgct gacactggcg
    20941 ttatcttcac tctcttccag ctgtattttc cggatgcgta gctgtccatc gtccagccag
    21001 ataaaagcgc tgccgattcg ctgatcgtcc tggcgaccct ggctaccggc gagatgaata
    21061 tagagcggga cgctcatgcc gtcctgaagc gcctgactga aggcctgcgg aataatcacc
    21121 ccacctattt gctgcgcact tatgtcagca gcgctggcat cgggctgaac gaacaaaaag
    21181 accatgccga aggcaaactg ggctttcagt cctggggaga accgtcgtaa aggcattgtc
    21241 gttatccatc tgcacagcta tctatttcac gggaatgaac ttatcaccct gccaaagtgc
    21301 aacccgtcct ttgttatccg ccgtgtccac gcgggtaaaa cgacgcgact tgcccggcat
    21361 taggtagtaa ttctctttac actctttacc attggcggct ttcaggcagg ggccgtaggc
    21421 gaggatccgc agcgtcgcat ttcctgtatt tgtcagggag ccgttggcgt actgaaagtg
    21481 gtagtttgcc tggcggggtg cgacgaccag aatggtgccg atgcgggcgg aagcagtggc
    21541 cacagcgctg cggttggcat tatcgcgctg tgcatcactg agggcctgat caaaccagac
    21601 aatgcggtag tagcgctctt tttcatctgc gggtccctta tagaagaagc ggatcacttc
    21661 gctggcttgg gcgggtagca gcaagctggc gggagtgagt agcaactcat ccggcttgtc
    21721 cattgagata acctgcccgt cgtcaagcgg tgaagagagc cgttcgagac ggatattgat
    21781 aaggcgacca ctgtcggtac tgtttttgat cgttttgctc agcgtgctgc tgtcactgtt
    21841 cataaacgat gatatatcgc cgacatccag cgcctgggcc ggagatattc cggaaaacag
    21901 cagagcgaga ggcagaaggt gctttttcat aacaattccg tccaggaata aagcaggggg
    21961 gctacccctg ctggtacatc agagagatta actggtccag gtcgcgtcga actgtacgct
    22021 aacgtcgccg ctccagatgc cttccggtag agtgctgtaa tcggttactg cggtggtacc
    22081 attggtggtg ccgctgatga tggtgaaggt gaaaccatcc tgtgcggtgg tacgattgct
    22141 ggcattgtaa ccgttagcca gcgggctaag gttgccgccc agtacgccgt tggcggtatc
    22201 gatcatcacg gtatcgccag ttttttcgac tgccgcgccg ttataatcca cgcccacatt
    22261 cagtgtggaa cctgaggtat ccaactgggt taatgtgttg gtgataagac gtgaggtcag
    22321 tttaaaggcg gtagccgttg agtcaccctc gatagccacg tcaaatagac ctttctgtga
    22381 gttaaaacct ttaatgcctt cggcatactg gaacgccagg ctaccgagtg gcgtcacaac
    22441 cagcttactg gtggtgtctt ttttggctgt tgccgaccag gtcgctacag cctgagctgt
    22501 tacgtcagca gcctgcgcca cacccatgcc ggtaaacacc gttaccagag ctattgccag
    22561 aacctttttt ttcattgcat ttcttcccga gttgaattga ggacgtgatg tcccagtgag
    22621 tcatttttta aaactaactt gcctggagtt tactgaacca acttatatat ttttgagtac
    22681 agcttggcct cagcattacg ccgatgggta tacactgtct tcacactaca attttcaatt
    22741 ctggcaatcg atttaggttg cattccctgg gccgtcatgc ggataatttc catttcccgg
    22801 tccgtgattt tatctttctt aatatctttt ctcaggaacc tgccattaat cacataggcc
    22861 agttcttttc taatatcacg actcagtcca gcgtatacca cgcccctaat attgctattg
    22921 taataccaat aatttgctaa ggcttctgac tttctggccg caatcaatac gacctgtttc
    22981 ccctttaccg cggataacca ttcattatca cggtttataa attcagataa agaatccgta
    23041 ctgagattga taaacacgaa atcttttcta attttctcca acgatagata aattaatctg
    23101 tcgatatcca caatcagttc agatagtcct ttatagaagt aggcgtcatg gggagaccag
    23161 atgtatttat cagaacggtt ttgacattcc atatggtttt taacctcata gtccctactg
    23221 taatcacttt gccatgtcac tactttccaa acctgtaatt tttacaggtg cattcagttt
    23281 ccttagtaaa ccggaagtaa
//
LOCUS       DAFNBJ010000072        23270 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000072 DAFNBJ010000000
VERSION     DAFNBJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.072"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..3858)
                     /gene="entF"
                     /locus_tag="L2X49_002639"
     CDS             complement(<1..3858)
                     /gene="entF"
                     /locus_tag="L2X49_002639"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBN4797708.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDVHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQARGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPI"
     gene            complement(3855..4073)
                     /gene="ybdZ"
                     /locus_tag="L2X49_002640"
     CDS             complement(3855..4073)
                     /gene="ybdZ"
                     /locus_tag="L2X49_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HBN4797709.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            complement(4076..5278)
                     /gene="fes"
                     /locus_tag="L2X49_002641"
     CDS             complement(4076..5278)
                     /gene="fes"
                     /locus_tag="L2X49_002641"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBN4797710.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRGDRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5521..7761
                     /gene="fepA"
                     /locus_tag="L2X49_002642"
     CDS             5521..7761
                     /gene="fepA"
                     /locus_tag="L2X49_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBN4797711.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPLDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7936..8556
                     /gene="entD"
                     /locus_tag="L2X49_002643"
     CDS             7936..8556
                     /gene="entD"
                     /locus_tag="L2X49_002643"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBN4797712.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8678..8830)
                     /gene="hokE"
                     /locus_tag="L2X49_002644"
     CDS             complement(8678..8830)
                     /gene="hokE"
                     /locus_tag="L2X49_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBN4797713.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9283..10401
                     /locus_tag="L2X49_002645"
     CDS             9283..10401
                     /locus_tag="L2X49_002645"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBN4797714.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDILRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10467..10715
                     /locus_tag="L2X49_002646"
     CDS             10467..10715
                     /locus_tag="L2X49_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBN4797715.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVSTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYLVLRFIWRRWFSLAD"
     gene            10780..11148
                     /locus_tag="L2X49_002647"
     CDS             10780..11148
                     /locus_tag="L2X49_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBN4797716.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11242..11895
                     /gene="nfsB"
                     /locus_tag="L2X49_002648"
     CDS             11242..11895
                     /gene="nfsB"
                     /locus_tag="L2X49_002648"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBN4797717.1"
                     /translation="MDIISVALKSHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12003..13250
                     /gene="ybdG"
                     /locus_tag="L2X49_002649"
     CDS             12003..13250
                     /gene="ybdG"
                     /locus_tag="L2X49_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBN4797718.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13318..14694)
                     /gene="pheP"
                     /locus_tag="L2X49_002650"
     CDS             complement(13318..14694)
                     /gene="pheP"
                     /locus_tag="L2X49_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBN4797719.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(14796..17939)
                     /gene="cusA"
                     /locus_tag="L2X49_002651"
     CDS             complement(14796..17939)
                     /gene="cusA"
                     /locus_tag="L2X49_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBN4797720.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(17951..19174)
                     /gene="cusB"
                     /locus_tag="L2X49_002652"
     CDS             complement(17951..19174)
                     /gene="cusB"
                     /locus_tag="L2X49_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HBN4797721.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19190..19522)
                     /gene="cusF"
                     /locus_tag="L2X49_002653"
     CDS             complement(19190..19522)
                     /gene="cusF"
                     /locus_tag="L2X49_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBN4797722.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(19546..20928)
                     /gene="cusC"
                     /locus_tag="L2X49_002654"
     CDS             complement(19546..20928)
                     /gene="cusC"
                     /locus_tag="L2X49_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBN4797723.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21085..21768
                     /gene="cusR"
                     /locus_tag="L2X49_002655"
     CDS             21085..21768
                     /gene="cusR"
                     /locus_tag="L2X49_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBN4797724.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAMIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            21758..23206
                     /gene="cusS"
                     /locus_tag="L2X49_002656"
     CDS             21758..23206
                     /gene="cusS"
                     /locus_tag="L2X49_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBN4797725.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5571 a         6358 c         5943 g         5398 t
ORIGIN      
        1 aatcggccca atttttacaa acgcccctgg agagataata tccacatgcg cacaatcctg
       61 acgatagata tccagctccg caatccacgg cgaccaggca cgttcgggac tcataccttc
      121 ctgaagtgtg cgctcagcaa caaataacgt tgcttttcca tcaaacggaa cgctatgggc
      181 ggtggttaac aagcgcacag catcagcgta gttgccttca atggtggtaa acaactccgt
      241 tgaagtactt ccctgctgtg ccgccaggaa ggcctcgcgt tcgcggttaa tctccgccag
      301 cacttccggg tccagaccat tagcctcttt ttcctgccag ttttgcgtct ctggcggcca
      361 ggtatccagc aaaccaagaa atgccacctg ttcgccacgg gcacgcagtc gcgccgcaat
      421 accctgcgcc agcgtaccgc caagggaata ccccagcagg taataagggc cgtgcggttg
      481 ttgctcaagt aacgttgcca gatgcgcttc gcagacttca tccaggtttg tcgctgtctg
      541 catgggtcca tgagggcgcg gcgactgaat gccaataatc gaccattgtg gatcgagata
      601 acgcgagagc acgctaaact gccaggcaaa accggacgcc ggatggaaac aaaacagcgt
      661 cgggccatta ccttcacgca acggcagaat ggtttcgaat cccatgcgcc gggagctgtc
      721 ctcttcacca tcaataatcg ttgccagttt ggcgacggtt gacgcgacca tcacctgccc
      781 cggcgtcacc tgacgagcaa actgtcgact taactgcgct gctagtttca ttgccagtag
      841 cgaatgaccg cccagcgcaa agaaatcagc atcggcatcc tgcacgtcac aacccagcaa
      901 cgacgcgaat gccgcggcga taatcgtttc actgcccgct ttcggtgcac gtcccgacgc
      961 ttgtgtcttc agttcaggca acggtaaggc tttgcgatcc agcttgccgt tggcgctaag
     1021 cggcaactgc ggaagttgca gcagaacgac cggcaccata tgcggcggca atgtttcgcg
     1081 aagctgtgcc tgtaatgcgc tggtatccaa cggcagtccc gattgcgaca ccagatagcc
     1141 caccaactga cgcgcatcac caccggtggc tgccgcctgg ttaatcacac aggcgtgggt
     1201 aactgcctgt tcgacatccg gcagcgcctg catcacgcga tcgatttcgc ccagttcgat
     1261 acgctgcccg cgaattttta gctgatcgtc actgcgcccg aggtactcca ccgcgccgtt
     1321 atccagccag cgggcgacgt ctccggtacg atacatccgt tcacctgggg caaaaggatc
     1381 ggcaataaaa cggctggcgg tcagatcggg tcgtccaaga tacccctgcg ccagttggat
     1441 gccggtgaga tagagatcac ccgccacacc cggcggcacc ggatgcatca tcgcatcaag
     1501 aatacgcaga cccgtattcc ataccggata accaatcggc acactgctgc cgcgcgcctg
     1561 tgccagttcc tcgccaaaag ccggatacca gctgacatct accgccgctt ccgtcgggcc
     1621 atacaggtta tgcaacggcg cgccggttaa ctgttgccat tcgcggcata aatcagccgg
     1681 taaggcctca ccactacaga aaacctgttt caacgtcgcg caattctggc gagcggtttg
     1741 cggcgtcagc gatgcaacaa atgccgccag catcgacggc acaaagtgcg tggtcgttac
     1801 gccatattcg gcaaagaatt gctgcatagc gagcgggtca cggtgcgctt ccggttcagc
     1861 catcaccagt tttgcccctg cgataaacgg ccagaaaaac tcccacaccg agacatcaaa
     1921 actgcacggc gttttttggg caacgacatc ttcgcctgta agcgggtagt gattttgcat
     1981 ccataacagg cggttaacga tagccgtctg cccgaccatt acccctttcg gcctgccggt
     2041 ggagccagag gtaaagatga tataagccgt gtggtgcggt tgtgaaagtt gcagcggcgc
     2101 actgccctgg ggggtaagcg gggcgttata gcaaaggctt gttaaattgg gaacatcgct
     2161 aaagcgcggc agttgatcgt cggtggtaat taacagcgac ggacgcgcat cttccagcat
     2221 cattttcagg cgatcgtccg gatagccggt atccagcggt agccaggccg cgccagcttc
     2281 aactattgca tggagtgcca gggtcaaaaa gaccgagcgc ggtagtgcca ccgccacgct
     2341 gtcccctggt ttaacgccgc gctcacgcag cagattcgcc agcgccacca cctgctcgcg
     2401 catttcccga tagctgaaca ggtaacgcgc atctgccagc gccggagcat ccggtgtttt
     2461 tgccgcttgt tctgccacca gcgcgctcag cgtggtttct ggaatctcaa cctgagtggc
     2521 gttgatctgc gccagctgcg catactcacc tggcagcata atatcgacat cgccgcacaa
     2581 cagcgccgga tccgcggcga actgggcaat cagcattttc aggcgttcag catgctggat
     2641 taacgttggc tcatcgtaac gctgtttatt ggcgaggatc tcaatactca aatcaccgtg
     2701 tacatccggg aacagggcca gttcaaggtc attaaccgga ccggttgcca gggtatgggt
     2761 ttgcgcctga acatcaggaa tatccagttg gtaatcaaat accttgatat tgagtaccgg
     2821 accaaacagc ggttcatcac ctgccgctcg cccgctgtca cggacaattt gttcggcatc
     2881 gtaacgttga tgacgacgca tttttttcag ttgtgctgcc agtcgggttg ccagttccgg
     2941 cagcgtttct tgcgccgcaa tgtgaatacc caacggcaaa acgttgagca cgggtccggt
     3001 agccgtcagc gccgccgagc ccagtcgacg cataaagata aatccggcgg cgtagtccat
     3061 acgattgcac aatcgcccca gccacaaggc tgccagcgca agggctaaat cggtacgctg
     3121 cacacctgaa agttgcgtag ccagctggcg gaattccccg tcggtaaatt ccagtttcag
     3181 gcgcagaata tctgccgagg cgctgcgccc cggtaaaggt gccggagaaa gtgacgcggg
     3241 cggcggcagt tgacgacgct gttctgccca gaatgccgca tcacgctgcc aggcttcgct
     3301 ttcgcggtat tgctggtact cttccactac atcagcgaaa ggcgtaaatg gcgaagcagg
     3361 cgttggttcg ccacgcagcc atgtgcagta aatattggcg atctggcggg taatggccgg
     3421 gaaactgaag ccatcgacca gcaaatggtg ataacgctga taccagtacc agcggttatc
     3481 cgccacctgt atcagctgat gaaagaccag tggtttaccg ctatcgacgc gcagatcttg
     3541 ttgcaaatcc gcctgcatta atgcctgcgc agtaccgtgc ggatcaatgt tggttcgtag
     3601 gtcgataatt tctggcagtt cgaacgtcag cgcatcatcg acccactgcc agacttcgcc
     3661 gttatcttcc gtaaaacgca tccgcagcgt atctgcttgc gctagtccgg caaccaccgc
     3721 gcgggccagt aatggcgaat caacctctcc ggttaactca acgtaatgcg ccacgctcca
     3781 ggcggagggt aattctgaca gtttttctgc catccagatg ccgggctgtg cggcgaccaa
     3841 aggtaaatgc tggctcattg tgcctcctgc aactgggtaa aattcgtcgg tgtcagagta
     3901 cgccagtggg cttccagcca ctgctggcag gacgcctgtg actgcggctg acacacaatg
     3961 tcccagcctg ccggtaaggc gcattgttgc ggccacagac tgaattgccc ctgcgcattg
     4021 cgcaaaatgt aaaacgctcc ctgcggatca tcgaagggat tactgaatgc catattcaac
     4081 tcctgtcatg gaaaagtggt tgccagaggt cgattagccc ctgcatcaag ccaccgcgcc
     4141 aacaaagcgc atcatgtccg ccgtcaacct gacgccagaa aatggattct tttatggggt
     4201 gtaattgcgc atacagcgcc tgattggccc gcataatcat cggctcgcga atacccgctt
     4261 ccagcacaat gcgcagacct tcggcgctaa cttcaccagc tttaagcttt tcaagtaaca
     4321 cgccctcttg ctgcccgccc cgatgcggcc accagtacga tcctgactgg cttaatacac
     4381 agccaaagcg ttcaggccag tgcagtccgg catacagcgc ggacagccca ccaaaactct
     4441 gcccggcaac cacggtgcga tcgccgcgat cgctaaaagg ggcaatagct ttcaccaggg
     4501 gtaataactc ttgctgtact gccagccaga aatccgcatt acacggcagt tcgtgggcgc
     4561 ggtgcgtggt gtcgatagcg tcgatcaaca catacacggc gggaggaagt tgctgacgat
     4621 gggtcagcga agtcagcaca ggccagacgg gcatactttg cgcccaaaat tcgccatcga
     4681 gcaaaactgc cagcggacgt tcttctgctg ttacatcgcc ggtggtaaaa atccatacac
     4741 gacgtgaatt tttcaaccgt tcacttttcc agataatttc tttggctgct atttctggcg
     4801 cttgcggaca atcccatccc ggttgcagag gcgcttgcgg catttcgagt gcagaaacag
     4861 cgtgccctcg cccgcctttc cagctttgtg gattcagcgg atcggctatt gcctggggta
     4921 atagttttcg ccagccttca cgtaattcga ggcgatcggg ggatggtgca gaaaaaatgt
     4981 catcgcgttc ggtgggaata aagcagtagc tgccgcgcca gttggcattg agttgtgtcg
     5041 tccactgcca gacatcagta cctgcaattc gctgcatcga ctggggctgg ctgttctgat
     5101 gatgatcggt cacaccagtg atgtagatcc atacgcgctt tatcggcgag tattcttcag
     5161 aaccttgggg atcacgccac cagaaagtga cctcaaacat ttcgtcattc agacgctgcc
     5221 attccgggcc atgtttcgac tgccaccagc tctcacttcc tacttttaac gccgtcacac
     5281 cataacccca tgtttactgt gcaatttttc attgattgca gaaatatatt gataatatta
     5341 ttgataacta tttgcatttg caatagcgta atggcgcgcc gtgggaagcg cggacattta
     5401 ttaaccaact gcactgcgtg tctttcagga tcaaaggttt tcgcggtagc gggatgcgtc
     5461 gtgttgagga cgaccatgcc cgacagttgc aattcgtggc aaaaatgcag gaataaaaca
     5521 atgaacaaga agattcactc cctggccttg ttggtcaatc tggggattta tggggtagcg
     5581 caggcacaag agccgaccga tactcctgtt tcacatgacg ataccattgt cgttaccgcc
     5641 gccgagcaga acttgcaggc acctggcgtt tcgaccatca ccgcagatga aatccgcaaa
     5701 aaccccgttg cccgcgatgt gtcggagatc atccgtacca tgccaggcgt taacctgacc
     5761 ggtaactcca ccagtggtca gcgtgggaat aaccgacaga ttgatattcg cggtatgggt
     5821 ccggaaaaca cgctgatttt gattgacggc aagccggtaa gcagccgtaa ctcggtgcgt
     5881 cagggctggc gtggcgagcg cgatacccgt ggtgatacct cctgggtgcc acctgaaatg
     5941 attgaacgta ttgaagttct gcgtggtccg gcagctgcgc gttatggcaa cggcgcggcg
     6001 ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggtg agtggcacgg ctcctgggac
     6061 gcttatttca atgcgccaga acataaagag gaaggtgcca ctaaacgcac caacttcagc
     6121 ctgaccggtc cgctgggcga cgaattcagc ttccgcttgt atggcaacct cgacaaaacc
     6181 caggctgacg cgtgggatat caaccagggc catcagtccg cgcgtgccgg aacgtatgcc
     6241 acgacgttac cagccgggcg cgaaggggtg atcaacaaag atattaatgg cgtggtgcgc
     6301 tgggacttcg cgcctctgca atcactcgaa ctggaagcgg gttacagccg ccagggtaac
     6361 ctgtatgcgg gcgataccca gaacaccaac tctgacgctt atactcgctc gaaatatggc
     6421 gatgaaacca accgcctgta tcgccagaac tactcgctga cctggaacgg tggctgggat
     6481 aacggtgtga ccaccagcaa ctgggtgcag tacgaacaca cccgtaactc gcgtattccg
     6541 gaaggtctgg cgggcggtac cgaagggaaa tttaacgaaa aagcgacaca ggatttcgtc
     6601 gatatcgatc ttgatgacgt gatgctgcac agtgaagtta acctgccgct tgatttcctc
     6661 gttaaccaga cgctgacgct gggtacggag tggaatcagc aacggatgaa agacttgagt
     6721 tccaataccc aggcgctgac cgggacgaac accggtggcg ctattgatgg cgtgagtgcc
     6781 accgaccgta gcccgtattc aaaagcagaa attttctcgc tgtttgccga aaacaacatg
     6841 gagctgactg acagcaccat cgtaacgccg gggctgcgtt tcgatcatca cagtattgtc
     6901 ggcaataact ggagcccggc gctgaacata tcgcaaggtt taggcgatga cttcacgctg
     6961 aaaatgggca tcgcccgtgc ttataaagcg ccgagcctgt accagactaa cccgaactac
     7021 attctctaca gtaaaggtca gggttgctat gccagcgcgg gcggctgcta tctgcaaggt
     7081 aacgatgacc tgaaagcaga aaccagcatc aacaaagaga ttggtctgga gttcaaacgc
     7141 gacggctggc tggcgggcgt cacctggttc cgtaacgatt atcgcaataa gattgaagcg
     7201 ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc tctatcagtg ggataacgtg
     7261 ccgaaagcgg tggttgaagg tctggaagga tcgttaaacg taccggttag cgaaacggtg
     7321 atgtggacca ataacatcac ttatatgctg aagagtgaaa acaaaaccac gggcgaccgt
     7381 ttgtcgatca tcccggagta tacgttgaac tcaacgctga gctggcaggc acgggaagat
     7441 ttgtcgatgc aaacgacctt cacctggtac ggcaagcagc agccgaagaa gtacaactat
     7501 aaaggtcagc cagcggttgg accggaaacc aaagaaatta gtccttacag cattgttggc
     7561 ctgagcgcga cctgggatgt gacgaagaat gtcagtctga ccggcggcgt ggacaatctg
     7621 ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca cgggcgattt ggcaggggcc
     7681 aactatatcg ccggtgccgg ggcgtatacc tataacgagc cgggacgtac gtggtatatg
     7741 agcgtaaaca cccacttctg atgctaacgt cagattgttg acaaagtgcg cgtcgttcat
     7801 gccggatgcg gcgtgaacgc cttatccggc ctacaaaaat cttgccaatt caatatattg
     7861 caggaccatg ttggcctgat aagcgtagcg catcaggcag ttttgcgttt gtcatcagtc
     7921 tcgaatatgg tcgatatgaa aactacgcat acctccctcc cctttgccgg acatacgctg
     7981 cattttgttg agttcgatcc ggcgaatttt tgtgagcagg atttactctg gctgccgcac
     8041 tacgcacaac tgcaacacgc tggacgtaaa cgtaaaacag agcatttagc cggacggatc
     8101 gctgctgttt atgctttgcg ggaatatggc tataaatgtg tgcccgcaat cggcgagcta
     8161 cgccaacctg tctggcctgc ggaggtatac ggcagtatta gccactgtgg gactacggca
     8221 ttagccgtgg tatctcgtca accgattggc attgatatag aagaaatttt ttctgtacaa
     8281 accgcaagag aattgacaga caacattatt acaccagcgg aacacgagcg actcgcagac
     8341 tgcggtttag ccttttctct ggcgctgaca ctggcatttt ccgccaaaga gagcgcattt
     8401 aaggcaagtg agatccaaac tgatgcaggt tttctggact atcagataat tagctggaat
     8461 aaacagcagg tcatcattca tcgtgagaat gagatgtttg ctgtgcactg gcagataaaa
     8521 gaaaagatag tcataacgct gtgccaacac gattaattga caacatctgg tacgattcgc
     8581 ccgcagccat cactgaccac gggcgaaagt gtaaagcagg tgccttacca tcctgacctg
     8641 acaaccggat atgcggggat tgctccccgc acaacggcta cttcttcggt tcgtaagcga
     8701 gaacagcctt aaactcaata ttacgttcct ttacggtgaa ctcacacagc gaatctccga
     8761 ccagaagcgt aaatcccagc accgttaaac acagcactat gactgccgca agggcatatt
     8821 tcgtcagcat gtttatattg cctccatgcg aagaagggac taccatccaa attgttcggg
     8881 tttggagtgg cagccccagg gttgatagaa atatctcctg gggcttcatc tttccagacc
     8941 tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc gttatcttac ccttcctcaa
     9001 caaaatagct gtttatttat acagcgagtc ctgtttcgga cttctcactc ctcatttaat
     9061 caactgaagc tgaacccgtt tttttctgca tgactcaatc gtcaaaattc ttcgccacca
     9121 gtcagcactg ccataacttg tcacattgca aaagtagcca aattgcttca ggcaccatca
     9181 gttcaccatc accaaaaaaa agaaacattc cagaacttcc gcatctcgcc cagccgctcc
     9241 gacttctata ctgaatagaa aacgccaaca taagagaaac ctatgccatt acccgatttt
     9301 catgtttctg aaccttttac cctcggtatt gaactggaaa tgcaggtggt taatccgccg
     9361 ggctatgact taagccagga ctcttcaatg ctgattgacg cggttaaaaa taagatcacg
     9421 gccggagagg taaagcacga tatcaccgaa agtatgctgg agctggcgac ggatgtttgc
     9481 cgtgatatca accaggctgc cgggcagttt tcagcgatgc agaaagtcgt attgcaggca
     9541 gccacagacc atcatctgga aatttgcggc ggtggcacgc acccgtttca gaaatggcag
     9601 cgtcaggagg tatgcgataa cgaacgctat caacgcacgc tggaaaactt tggttatctc
     9661 attcagcagg cgaccgtttt tggtcagcat gtccatgttg gctgcgccag tggcgatgac
     9721 gccatttatt tgctgcacgg cttgtcacga tttgtgccgc actttatcgc cctttccgcc
     9781 gcgtcgccat atatgcaggg aacggatacg cgttttgcct cctcacgacc gaatattttt
     9841 tccgcctttc ctgataatgg cccgatgccg tgggtcagta actggcaaca atttgaagcc
     9901 ctatttcgct gtttgagtta caccacgatg atcgacagca ttaaagatct gcactgggat
     9961 attcgcccca gtcctcattt tggcacggtg gaggttcggg tgatggatac cccgttaacc
    10021 cttagccacg cggtaaatat ggcgggatta attcaggcca ccgcccactg gttactgacg
    10081 gaacgcccgt tcaaacatca ggagaaagat tacctgctgt ataaattcaa ccgtttccag
    10141 gcctgtcgct atgggctgga aggcgtcatt accgatccgc acactggtga tcgtcgaccg
    10201 ctaacggaag atatcttgcg attgctggaa aaaatcgccc cttccgcaca taaaattggc
    10261 gcatcgagcg caattgaagc actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag
    10321 ctgatgcgcg atttcgtcgc cgatggcggc tcgctgattg ggctggtgaa aaagcattgt
    10381 gagatctggg ccggtgacta agtcgggaat tgccttacag aagaatatcc ctgacaatgg
    10441 tgttttaact tacatttaac aatgttatga aacacccttt agaaaccttg accaccgcag
    10501 caggcatttt gctgatggct ttcctctctt gcctgctgct tcccgccccc gcattggggc
    10561 tgacgctggc acaaaaactg gtgagcacgt tccatctgat ggatcttagt cagctttaca
    10621 ctttattgtt ttgtctgtgg tttttagtgc tgggcgctat tgagtatctt gttctgcgct
    10681 ttatctggcg acgctggttc tcgctggcgg attaaacgtc gattatcgcc gtacttcgcg
    10741 gcatactttg cttattctcc ttcgcctaaa ggaacgttta tggataagca atcactgcac
    10801 gaaacggcga aacgcctggc ccttgagtta ccctttgtcg agctttgctg gccttttggc
    10861 ccggagttcg atgtttttaa aattggcggc aagattttta tgctgtcgtc ggagctacgc
    10921 ggcgtcccct ttatcaatct gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata
    10981 tacccaagca ttaagccagg gtatcacatg aataaaaagc actggatttc ggtgtatccc
    11041 ggcgaggaaa tctccgaagc gttacttcgc gatctgatca acgattcgtg gaatctggtg
    11101 gttgatggtc tggctaaacg cgatcaaaaa agagtgcgtc caggctaaag cggaaatcta
    11161 tagcgcattt ttctcgctta ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa
    11221 ttactttcac atggagtctt tatggatatc atttctgtcg ccttaaagag tcattccact
    11281 aaggcatttg atgccagtaa aaaacttacc ccggaacagg ccgagcagat caaaactctc
    11341 ctgcaataca gcccatccag caccaactcc cagccgtggc attttattgt tgccagcacg
    11401 gaagaaggta aagcgcgtgt tgccaaatcc gctgccgata attatgtgtt caacgaacgt
    11461 aaaatacttg atgcctcgca cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc
    11521 tggctgaagc tggttgttga ccaggaagat gctgatggcc gctttgccac gccggaagcg
    11581 aaagccgcga acgataaagg tcgcaagttc ttcgctgata tgcaccgtaa agatctgcat
    11641 gatgatgcag agtggatggc aaaacaggtt tatctcaacg tcggtaactt cctgctcggc
    11701 gtggcggcgc tgggtctgga cgcggtaccc atcgaaggtt ttgacgccgc catcctcgat
    11761 gcagaatttg gtctgaaaga gaaaggctac accagtctgg tggttgttcc ggtaggtcat
    11821 cacagcgttg aagattttaa cgctacgctg ccgaaatcac gtctgccgca aaacatcacc
    11881 ttaaccgaag tgtaattctc tcttgccggg catctgcccg gctatttccc ctcagattct
    11941 cctgatttgc ataaccctgt ttcagccgtc atcataggct gctgttgtat aaaggagacg
    12001 ttatgcagga tttaatatcc caggttgaag atttagcggg tattgagatc gatcacacca
    12061 cctcgatggt gatgattttc ggtattattt ttctgaccgc cgtcgtggtg catattattt
    12121 tgcattgggt ggtactgcgg accttcgaaa aacgtgccat cgccagttca cggctttggt
    12181 tgcaaatcat tacccagaat aaactcttcc accgtttagc ttttaccctg caggggatta
    12241 tcgtcaatat tcaggcggta ttctggctgc aaaaaggcac cgaagcggca gatattctga
    12301 ctacctgcgc gcagttgtgg atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg
    12361 atgttatttt gaatctggcg cagaaattcc cggcggcatc tcagttaccg ctgaaaggga
    12421 tatttcaggg gattaaactg atcggcgcaa tcctggtcgg cattttgatg atctcgctgc
    12481 tgattggtca gtcgccagcg attctgatca gcggtcttgg tgcaatggct gccgtgctga
    12541 tgttggtttt taaagatccg attcttggtc tggtggcagg tattcagctt tccgcgaacg
    12601 atatgctgaa actgggcgac tggctggaga tgccgaaata cggcgcggat ggcgcggtga
    12661 tcgatattgg gttaaccacc gtcaaagtgc gtaactggga caataccatt accactattc
    12721 ccacctggtc tctggtttct gactccttta aaaactggag cgggatgtca gcatctggcg
    12781 ggcgacgtat taagcgcagt atcagtattg atgtcaccag tattcgtttt cttgatgaag
    12841 acgaaatgca acgtctgaat aaagcgcact tgttaaaacc ttatttaacc agccgccatc
    12901 aggaaattaa tgagtggaat cgccagcaag gttctacgga gtcgatatta aacctgcgcc
    12961 gaatgaccaa tattggaacc tttcgtgcct atctgaacga atatctgcgt aaccatccgc
    13021 ggattcgtaa agatatgacc ttaatggtac gccaactggc tccaggtgat aacggtttac
    13081 cgctcgagat ctacgcgttt actaacaccg tggtgtggct ggaatatgaa agcattcagg
    13141 ctgatatatt cgatcacata tttgcgattg tcgaagagtt tggtctgcga cttcatcagt
    13201 cgccaaccgg caatgatatt cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac
    13261 cgctctcatc gaatggatga gagcggacat cgttttgtcg gatgtagcgt gaatgcctta
    13321 tttccgacgc agcgttttaa atgccacaaa caggaataca atccacaccg gcagcaggat
    13381 cgctgacaag cgcatatcat ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc
    13441 aatgcaaaga tagttgccga acggatagag cagcgcctta aactgtgttt cacgcccctg
    13501 acgtcgcatc gctgcacgaa aacgcagatg cgccagacag atcataatcc agttcaacag
    13561 cagcgttgct accaccagcg ccatcagcag accaaacgct ttttgcggca gcagatagtt
    13621 gattaatacc accagcgaag tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc
    13681 gcgacggctg acgcgagtca aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag
    13741 catgcggctg ttagagtaaa ccccgctgtt atacactgac agcgatgcta ccagaatgac
    13801 gaagttcagc gcagaagcta ccacgttgct gtcgagatta tggaaaatca tcacaaacgg
    13861 gctactgttg gatttcactt ccacccacgg atagagcgcc agtaaaacca ccagtgaacc
    13921 gatgtaaaac agcaggatgc gatacaccac ctgatttacc gcttttggaa tgcttttttc
    13981 cggatcgcgc gcttcagcgg cagtaatccc aatcagctcc agaccgccga aggagaacat
    14041 aattaccgcc agcgacaaaa tcagcccatt ccagccggtg gcaaagaaac caccgtagcg
    14101 ccagaggttg tcgatactgg ctttctcgcc gccgtgacca gaaaacagca gccacaggcc
    14161 aaagccgatc ataccgatga tcgccagcac tttaatcagc gcaaaccaga actcggtttc
    14221 accatataag cgcacgttca ccaggttaac ggcgttgatg ataataaaga aggcggcagc
    14281 ccaaatccac gttggaacat ccgggaacca gtactgcata tagatgcccg cagcggtcag
    14341 ctctgccatt cccaccagca cgaacattac ccagtagttc cagccagaga ggaagcccgc
    14401 aaacggtccc cagtatttat aggcaaagtg ggcaaatgaa ccggataccg gctcctcaac
    14461 caccatttcg ccaagctggc gcataatcag gaaagcgatg atcccggcga cgccgtagcc
    14521 cagcaataca gccggacccg ccatctgaat cgccgggcca atgccaagaa acagaccagt
    14581 accaattgcg ccacccagcg caatcagttg aatatgacgg ttatgtaatc cgcgatgaag
    14641 cgtcggctct tgattcgacg cagtatcttc cgatacggtt gacgcgtttt tcacgccttt
    14701 cccctgtgtg tcttttttgt tgaggggcac cttttaacat ttagtgccca tcgtcgcaag
    14761 acacaatcca cacggttaaa cggggtatcc tgcttttatt tccgtacccg atgtcggtgc
    14821 agccacatca gcttatacgc cgccgggata ataaacagcg acagcaaagg tgcggtgatc
    14881 atgccgccaa tcatcggcgc ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc
    14941 cacagaatcg gcagcagacc ggcgataatc accgccaccg tcatcgcttt cgggcgcacg
    15001 cgcaggaccg cgccgtgata taacgcctca tccagcttct gctcgctgaa tgtttgtgga
    15061 ttattcaacg acggcacggc ctctatggcg tgacgtaaat acatcagcat caccacgcca
    15121 aattcggcgg cgaccccggc gagggcgata aagccagtgc ccgtcgccac ggaaagatga
    15181 aagcccatcc accacagcaa ccagatgccg cccaccagcg caaacggtac gctgctgata
    15241 atcagcaacg cttcgcccac ccgacggaac gccagataca acagcacgaa gataatcatc
    15301 aacgtcatcg gcaccatgag tttaagctta tggttggcgc gctccagtag ctcgaactgc
    15361 ccggagaatg ccacgctggt gcccggtttt aactgcactt tttcagctat cgctttttgc
    15421 aaatcgtgaa ccaccgacac catgtcacga tcgcgggcat cgatataaat ccagctcgtc
    15481 gggcgcgcat tctcggtttt cagcatcgac ggtccggtag agactttaat gtcggccacg
    15541 tctgccaggg tgatttgctg cttcatcggc gtcaggatcg gcagctggcg cagtgcctgc
    15601 ggactatcgc gccagctttg cggataacgc agattaattg gataacgggc aatcccttcc
    15661 accgtttcgc caaccatcgc cccgcccacc gcagaagtca caaacaactg cacatccgcc
    15721 accgtcatac cgtaacgtgc ggctttttca cggttaatct caacgttgat atagcgccca
    15781 ccttccagcc gctcggcaag cgcagaagct acgcctggca ccgttcgcgc tacttcttca
    15841 atttgctcag ccatcgcgtc gatatccgcc agcacagtgc cggaaacttt aatgccgatg
    15901 gggcttttaa tgccggttga gagcatatcg atacggttac gaattggcgg cacccacaga
    15961 ttcgccagcc ccggcagccg cacggtgtta tccagttcct caatgatttt gtccatcgtc
    16021 atgcctggcc gccactgctc ctgcggctta agctggatgg tcgtttctac catctccagc
    16081 ggagcagaat cggtggcggt ttccgctttc ccggttttgc caaatacccg cgccacttca
    16141 ggtacgctca taattagctt gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa
    16201 atccccggca gcgtcgatgg catatacaac aagtcgcctt cattgatctg cggtaaaaat
    16261 tccccgccaa ctttattgag cggccagaga accgtcagca ccgaaagcgc cgccaccagc
    16321 agcgtggttt tcggccagtg cagtactttc agcaacagcg gatgataaac acgaatcaaa
    16381 aagcgattga gcgggttact gctttccggc ggaattttgc cacggatcca gtagcccatc
    16441 aggatcggga tcactacgat cgccagcagc gccgcacccg ccatcgcata cgttttggtg
    16501 aacgccaacg ggccaaacag acgcccttcc tgcccttcca gggtgaagat cgggataaac
    16561 gacaacgtga taatcagcag actgataaat agcgccggcc ccacttcaac agacgcatcg
    16621 gtgatcacct gccagcgcgt tttattatcc agcgtggcgt caggatgctg gtgctgccac
    16681 tcttccagcc gtttatgcgc attctcgatc atgacgatag cagcatcgac catcgccccg
    16741 acggcaatcg caatgccacc caacgacata atattggcat tcagtccctg gaagtgcatg
    16801 acaataaaag caatacacaa ccccagcggc aacgaaataa tcgccaccag cgccgagcgc
    16861 acatgccaga gaaacagcgc acagactacc gccaccacaa taaactcttc cagcaacttg
    16921 ccgctgaggt tgtcgatggc gcggtcaatg agctggctgc gatcgtatgt tgtaactatc
    16981 tccacgcctt ccggcagact acttttcagc gtttccagtt tgtccttcac ggcggcgatc
    17041 acttctcggg cgtttttgcc ggatcgcagg atcaccaccc cgcccgccac ttcgccttcg
    17101 ccgtttagtt cggcaatgcc ccggcgcatc tccgggccaa tctggacctt cgcaacatcg
    17161 cgcagataaa cgggcacgcc attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg
    17221 agcgtttgca gatagccgct ggcgcgcacc atatattccg cttccgccag ttcgatcgac
    17281 gaaccgcccg cttcctggtt tgaagcatcc agcgcgcttt ttacttcggc gagactgatg
    17341 ccatactgcg ccaggcgctg gggatcgata accacctgat actctttcac cacaccgccc
    17401 accgacgcca cttccgcaac gtcagggatg gttttcagct catatttgag aaaccagtcc
    17461 tgtaatgagc gtaaatcggc cagatcgtgc ttaccgctgc gatccaccag tgcatattca
    17521 tagatccagc caacacccgt ggcatctggc cccagctcgg cgctgactcc cgcaggcagc
    17581 ttaccctgta cctggttgag atactccagc acccgcgaac gcgcccagta cggatcggtg
    17641 ccatcttcga aaatgacata cacataagag tcgccaaact gtgagaaacc gcgcacagtc
    17701 ttcgcgccag gcaccgacaa catggtggtg gttagcggat aagtcacctg attttcaacg
    17761 atttgtggtg cctgaccggg atagctggtt ttaataataa cctgcacatc ggagagatcc
    17821 ggcagcgcat ccactggcgt attaatgatg gtccaggtgc cccagatgct cagaaacaac
    17881 gcgcccatca gcaccaaaaa acggttcgcc accgagcgac gaataatcca ttcaatcatt
    17941 ggttattccc tcaatgcgca tgggtagcac tttcagagcg catccgctcc agtgcgccag
    18001 aaatattggc ttcggaatca atcaggaaca ggccgctgga aaccaccttt tcaccttccg
    18061 ccagaccaga gcgtaatgcg gtgacgcctt gcgatgcctg gaaaacagca acgcgtttcg
    18121 gtacaaagcg cccgtcggca tcaacggtaa tcacccgctg ttcgctgccg gtatcaatca
    18181 gcgcttgtga cggaatgagc agcatcggtt cgctggcggt gttgagttgc aaccaggcgt
    18241 tcatgcccgg ttttagcgcc tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc
    18301 gggtcgcggc atccacgcca ggtagcagcg tccatttgcg gatggtgagt gttttatccg
    18361 gtcgcgccgg aacggtgagg gtaaactgcg aggcatcttt caccagccag gcgatggact
    18421 ccgggatcgc agcagtgacc cacaccgggt ccataccctg aattttcgcg accacgttat
    18481 ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg
    18541 ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggcggcga atatccgcct
    18601 ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc gccgtaccgc
    18661 cggtttcgcg cagcagtaaa tactcactct gcgcttccac ccagtcagga atggtcaggt
    18721 cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga
    18781 taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg
    18841 ccgggaaact ctgggcaaaa gtcagcggtc cgcgcgtgac ggtagccgtt ttcaccccca
    18901 gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat
    18961 atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat
    19021 tgggatacat tgggtcgtac cagaataaga ttttacgttc tgcggtcgac gttttttctg
    19081 caggcggttc cgcctttgca acccaggtaa agcctgccgc agaaataata ccgcccgcga
    19141 tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga
    19201 ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt
    19261 cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct
    19321 ccggccagtt cacagcagca atcggatcgt gatggatggt gatttttttg ctttccagat
    19381 caataccctt tacctcgcca gtggcactaa taacctgtgg ttgtgcttcg ctcatggttt
    19441 catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg
    19501 cgacttgcag tgcttttttc atttattctc tcctggagtt aaaagttatt gctgccaacc
    19561 gccaccaagt gcggtatata aagaaatttc gttaacctga cgggcataat tcagatcaag
    19621 taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac
    19681 tgcgccgtgc tgatataatg cccgcgcccg ttgcaaagta atttgcagcg atgccagata
    19741 acgctgctgg gcgctaattt gatcgttcag gctttgacgt aatgccagcg catctgccac
    19801 ttctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct gctggcgaat
    19861 ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga tctcaatttt
    19921 gggtataaaa ttccacatcc cgctgctggc gttaaataat gacgatagat cgctactggc
    19981 ggtcgatatt ccgctggtca ggcttataga cggaaaaaat gccgcgcgtg cagcgccaat
    20041 attggcatta gccgccatta acgcgtgttc agcttccata atatccgggc gctgcaataa
    20101 gatttgcgac gacaatcccg ccggtaattt aacgctttgc aggctgtcgc tgtttactgt
    20161 ctgcgcttgc ggcagcttgc cgtagcttcc cagcaacagt tgcaatgcat tattcgcctg
    20221 cgccagttcc ccctgacgtt tagcgatgtc gctgcgggta ctttctatca ccccgcgagc
    20281 ctgttccagc gccagaacat tactgctacc tgtcaacagt tgtttttcga caaacgcata
    20341 tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagttg
    20401 ctgattgaaa tagctttgcg cgacattaga aaccagcaga atatgcaccg cgcgctgagc
    20461 ttcctcagtg gctaaataat tttgtcgctc ggcttcgctc atgttcttta agcgaccgaa
    20521 aaaatcgagg tcaaagctgg cgttaaggcc ggtcgaaaac tcccgtgtcg tggcggtatt
    20581 ccctttaaga ttaccgctcc agctgccgct gccctcgcca ttgagctgtg ggtagcggtc
    20641 ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa
    20701 atcccgatta ttcaccagcg cctcgctaat cagcgtcttc acctgattat caacaaaaaa
    20761 ggtgcgccag cccgcgttct gatagttatc tgctgcgtta accaggccgt tctggctgag
    20821 tgagaactgc tgcggcacgg gcattgccgg acgctgataa tccggtgcca gtgaacaacc
    20881 ggttagcgca agagccacac aaaatggcag aagtttacaa ggagacatag gctcataatt
    20941 tctggtgatt ttatgccgcc aactttactc gccaggctct gattttccgg tgacagaaaa
    21001 atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt
    21061 gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc
    21121 ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac
    21181 gggttgaatg gctaccatct ggcgatgacc ggtgattatg atctgataat cctcgatatt
    21241 atgctgccgg acgtgaacgg ctgggatatc gtgcgcatgt tgcgttccgc caataaaggg
    21301 atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag
    21361 ttgggggcgg atgactatct ggtgaagccg ttcgcttttg ctgaactgct ggcgcgggtg
    21421 cgcactctcc tgcggcgcgg ggcggcgatg attatcgaaa gtcagtttca ggttgccgat
    21481 ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat cactctgacc
    21541 agtaaagagt ttactctgct ggagtttttc ctccgccatc agggcgaagt gctgccccgc
    21601 tcgcttatcg cctcgcaggt gtgggacatg aatttcgaca gcgacactaa cgccattgat
    21661 gtagcggtga agcggctgcg cggcaaaatc gacaacgact ttgagccgaa gctaattcag
    21721 accgtgcgcg gcgtgggcta catgcttgag gtgccggatg gtcagtaagc catttcagcg
    21781 cccgttttcg ctggcaaccc gcctgacctt ttttatcagc ctggccacca tcgcggcgtt
    21841 tttcgccttt gcatggatca tgatccactc agtaaaagtg cattttgccg agcaggatat
    21901 taatgattta aaagagatta gcgccacgct tgaacgggta cttaatcacc ctgacgaaac
    21961 gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca acgtgttgat
    22021 ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc ctgatatccg
    22081 cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg tgtatctcct
    22141 ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac acagcaactg
    22201 gcggatgatt aacttgtcgg ttggcccgtt ggtggacggc aaaccgattt atacgctcta
    22261 catcgcgctt tcgatcgatt ttcatcttca ttacataaat gatttgatga ataaacttat
    22321 tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtactgttgg cggtacataa
    22381 aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct
    22441 cgacgttcgc cttgacccgc agaccgtgcc cattgagctg gaacagctgg tactgtcgtt
    22501 caaccatatg atcgagcgta ttgaggatgt ctttacccgc cagtccaatt tctcagcgga
    22561 tatcgctcac gaaattcgca cgccgattac caatctcatc acgcaaacgg aaatcgccct
    22621 cagccagtct cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagagct
    22681 gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg ataacaacca
    22741 gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt
    22801 tttcgaggcg ttagcggaag atcgcggcgt ggagttgcga tttgttggcg acgaatgtca
    22861 ggttgcgggc gatccgctga tgctgcgtcg ggcgttaagc aacctgcttt ctaatgccct
    22921 gcgttatacg ccgcccggag aggcaattgt agtgcgctgc cagacggtcg atcaccaggt
    22981 gcaagttacc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac cgcgattgtt
    23041 tgaccgtttc taccgcgttg acccttcccg ccagcgaaaa ggcgaaggta gcggcattgg
    23101 gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcag taacgtcaga
    23161 tgcgcggggg acaaggtttg tgattatgct gccggagaga gagtgattat taagttcaaa
    23221 ttcagggcgt agtgcatcac ctcctggtat tgttgatcaa caaattagag
//
LOCUS       DAFNBJ010000073        23230 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000073 DAFNBJ010000000
VERSION     DAFNBJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.073"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            32..1285
                     /locus_tag="L2X49_002657"
     CDS             32..1285
                     /locus_tag="L2X49_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4797726.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMATALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1682..2017
                     /locus_tag="L2X49_002658"
     CDS             1682..2017
                     /locus_tag="L2X49_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBN4797727.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            2110..2193
                     /locus_tag="L2X49_002659"
     CDS             2110..2193
                     /locus_tag="L2X49_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797728.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2293..3114
                     /locus_tag="L2X49_002660"
     CDS             2293..3114
                     /locus_tag="L2X49_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBN4797729.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3153..3482)
                     /gene="mdtI"
                     /locus_tag="L2X49_002661"
     CDS             complement(3153..3482)
                     /gene="mdtI"
                     /locus_tag="L2X49_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBN4797730.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3469..3834)
                     /gene="mdtJ"
                     /locus_tag="L2X49_002662"
     CDS             complement(3469..3834)
                     /gene="mdtJ"
                     /locus_tag="L2X49_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBN4797731.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIVGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            4246..5280
                     /gene="tqsA"
                     /locus_tag="L2X49_002663"
     CDS             4246..5280
                     /gene="tqsA"
                     /locus_tag="L2X49_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBN4797732.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(5305..6693)
                     /gene="pntB"
                     /locus_tag="L2X49_002664"
     CDS             complement(5305..6693)
                     /gene="pntB"
                     /locus_tag="L2X49_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBN4797733.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(6704..8236)
                     /gene="pntA"
                     /locus_tag="L2X49_002665"
     CDS             complement(6704..8236)
                     /gene="pntA"
                     /locus_tag="L2X49_002665"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBN4797734.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCAYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8760..9704
                     /locus_tag="L2X49_002666"
     CDS             8760..9704
                     /locus_tag="L2X49_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBN4797735.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            9890..11272
                     /locus_tag="L2X49_002667"
     CDS             9890..11272
                     /locus_tag="L2X49_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBN4797736.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGADILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            11309..12031
                     /gene="folM"
                     /locus_tag="L2X49_002668"
     CDS             11309..12031
                     /gene="folM"
                     /locus_tag="L2X49_002668"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBN4797737.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(12028..12363)
                     /locus_tag="L2X49_002669"
     CDS             complement(12028..12363)
                     /locus_tag="L2X49_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBN4797738.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            12492..13211
                     /gene="rstA"
                     /locus_tag="L2X49_002670"
     CDS             12492..13211
                     /gene="rstA"
                     /locus_tag="L2X49_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBN4797739.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            13215..14516
                     /gene="rstB"
                     /locus_tag="L2X49_002671"
     CDS             13215..14516
                     /gene="rstB"
                     /locus_tag="L2X49_002671"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBN4797740.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            14592..15521
                     /gene="tus"
                     /locus_tag="L2X49_002672"
     CDS             14592..15521
                     /gene="tus"
                     /locus_tag="L2X49_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBN4797741.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(15518..16921)
                     /gene="fumC"
                     /locus_tag="L2X49_002673"
     CDS             complement(15518..16921)
                     /gene="fumC"
                     /locus_tag="L2X49_002673"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBN4797742.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(17064..18710)
                     /gene="fumB"
                     /locus_tag="L2X49_002674"
     CDS             complement(17064..18710)
                     /gene="fumB"
                     /locus_tag="L2X49_002674"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBN4797743.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            18909..20084
                     /gene="manA"
                     /locus_tag="L2X49_002675"
     CDS             18909..20084
                     /gene="manA"
                     /locus_tag="L2X49_002675"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBN4797744.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFSIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCQGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20185..21693
                     /locus_tag="L2X49_002676"
     CDS             20185..21693
                     /locus_tag="L2X49_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBN4797745.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(21738..23003)
                     /gene="uidC"
                     /locus_tag="L2X49_002677"
     CDS             complement(21738..23003)
                     /gene="uidC"
                     /locus_tag="L2X49_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBN4797746.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(23042..23230)
                     /locus_tag="L2X49_002678"
     CDS             complement(23042..23230)
                     /locus_tag="L2X49_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBN4797747.1"
                     /translation="MQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVV
                     EIDNRKKMQQQLISDITN"
BASE COUNT         5722 a         5970 c         5617 g         5921 t
ORIGIN      
        1 gatccacagg catatttagc aaggatttca agtgagccgt actacaactg ttgatggcgc
       61 tccggcaagc gacactgaca agcaaagcat ttctcagcca aatcaattta ttaaacgcgg
      121 tacgccgcaa tttatgcgcg tcaccctggc gctgttctct gccggactgg caacatttgc
      181 acttctctat tgtgtgcagc ctatccttcc ggtgctttcg caggagtttg gcttaacgcc
      241 cgcgaacagt agtatttcac tgtccatttc cacggcgatg ttggctattg gtttgctgtt
      301 tactggcccc ctatccgatg ccattggtcg caaaccagtg atggccacgg cgctactgtt
      361 ggcctccatt tgtacgttac tttcgacaat gatgaccagc tggcacggca ttttgattat
      421 gcgcgccttg attgggcttt cgttaagtgg cgtggcagct gttggcatga cttatcttag
      481 cgaggaaatc caccccagtt tcgtggcctt ttcgatgggg ttgtatatca gcggcaactc
      541 aattggcggc atgagcggac gcttaattag tggtgtcttc acggactttt tcaactggcg
      601 aattgctctg gcggcaatcg gttgtttcgc gctggcctcg gcgttgatgt tctggaaaat
      661 ccttccggaa tcacgccatt ttcgcccgac ttcgctgcgc cctaagacgt tatttatcaa
      721 ctttcgtctg cactggcgtg accggggatt accgttattg ttcgcagaag gctttttgct
      781 gatggggtcg ttcgtcacgc tgtttaatta catcggctat cggttgatgc tttccccctg
      841 gcatgtcagt caggccgtgg ttggcttatt atcgctggct tatttgaccg gtacatggag
      901 ctcacctaaa gccggaacca tgaccacccg ctacgggcgc ggtccggtga tgttgttttc
      961 gacgggggtt atgctgtttg gtttactgat gaccttattc agctcgctgt ggctgatctt
     1021 tgccggaatg ttactcttct cagcaggatt cttcgcagcc cactcagttg ccagcagctg
     1081 gattggcccc cgcgcaaaac gcgctaaagg ccaggcctcc tcgctgtatc tgttcagtta
     1141 ctatctgggg tcgagtattg ccgggacgct gggtggtgtt ttctggcata actatggctg
     1201 gaacggcgtc ggcgcattta ttgctctgat gctggtcatt gctctgctgg tcgggacgcg
     1261 tttgcatcgt cgtctgcacg cctgaaaaat aagcccggac tgcggtaact acacgtccgg
     1321 acttattgct tgctcaaacc aacgttaata gccatcctaa aatagacgaa gcgccagcca
     1381 attcccgcag cgcgtctagc gtcatcagga ttataagtac ccaaataaac ggattcattt
     1441 tgctgtgtgt catttattac tgatgcgcag ttattctacc gctttgtaag tagtaaaata
     1501 gttaacccga tcaagactac tattattggt agctaaattt cccttaagtc acaatacgtt
     1561 attatcaacg ctgtaattta ttcagcgttt gtacatatcg ttacacgctg aaaccaacca
     1621 ctcacggaag tctgccattt ccagggatat agttatttca acggccccgc agtggggtta
     1681 aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta ttagctctgg ttgttgccgc
     1741 tgctatgggt ctgtcttctg ccgcctttgc tgcagagact gcgaccacac ctgctccgac
     1801 tgcgacgacc accaaagcag cgccggcgaa aacgacacat cataaaaaac agcataaagc
     1861 agcacctgcc cagaaagcgc aggcggctaa aaagcatcat aaaaatacga aagctgaaca
     1921 gaaagcccct gaacaaaaag cgcaggcagc gaagaaacac gccgggaaac acagccatca
     1981 gcaaccggca aaacctgctg cacaacccgc agcgtaagtt ttcaacagta atgctggcgc
     2041 gccccctcgc gcctgaaaat tacggtgcta agcgggtaac gtttagcacc gcctttagcg
     2101 gagggtagta tggtgggccg ttatcgcttt gagttcattc ttatcatcct tattttatgc
     2161 gcactgattg ccgcccgttt ttatctttcc tgactgtagt tatctgattt tactcccact
     2221 ttcatcccgt cccgtctata gtatttatga gggtttgctt ttaataatca taattaccca
     2281 ccagagtgtg atatgcgtac aaccattgct gtagtgttgg gtgcaattag tttgacgtct
     2341 gcttttgtgt ttgcagataa accagacgtt gccaaatcgg caaacgatga ggtcagcacc
     2401 ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca cgacccaatc accgtgggat
     2461 gcggttgggc aactggaaac ggccagcggc aatttatgta cggcgacgct gattgcaccc
     2521 aatctggcat taacggcagg acactgttta ttgacgccgc ctaagggtaa agcggataag
     2581 gcggtagcgc tgcgttttgt gtcaaataaa ggtctttggc gctatgacat ccacgacata
     2641 gaaggccgcg ttgatccgac actgggaaag cggttaaaag cagatgggga tggttggatt
     2701 gttcctcccg cagctgcacc atgggacttc ggcttgattg tgctgcgtaa tcccccttct
     2761 ggcattacac cgttgccgtt atttgaggga gataaagccg cgcttactgc cgcattaaaa
     2821 tcggctggtc gtaaagtgac tcaggcggga tatcctgaag atcatctcga tacgttatac
     2881 agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt cggtgatgtc acatcagtgc
     2941 gataccttgc cgggtgacag cggttcgcct ctgatgttgc ataccgatga cggctggcaa
     3001 ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc gctggcgcgc cgataaccgg
     3061 gccatttctg ttaccggttt tcgcgacaag ctggatcaac tgtcgcaaaa ataatgttca
     3121 aacgctgccc gacagtgcgg gcagcgtgtt catcaggcaa gtttcaccat gatcattcca
     3181 gccaacagca agaccaggcc aatccagcct ttacgattta accgttgacc aaacaagatc
     3241 caacctgcgg ctaacgtggc ggcaatacca aacccgcccc acaatgcata agcgacagac
     3301 aagtcgatcc cttttacggc ctgagaaagc gcactaaagg cagccagcac cgccgccagg
     3361 gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa atttcaaaaa gacgttagca
     3421 acgatttcca gcacgattgc caatgccagc caggcggcgt gaacccattc aaactgcgcc
     3481 atggttcacc tccagttcag gtttacgcgc tttacgggta cctgatttga tcaacacaat
     3541 cccggcgacc agggtggtta acccgacaat tttcatcagc gataaacttt cgtcgaataa
     3601 caaaacgcta aacaaggtaa taaataaaat accgatacct tcccacagcg cataagctac
     3661 gcctaaggcg atctttttaa cggcgaaaga gagaaatata tacgacagag aaatcatcac
     3721 cagcattaaa ataaagccgc catttccctc actgacgctc gcccatttca ttgacagcgt
     3781 accggtaatt tctgtagcaa tagccaggcc taataaaatc caataaatat acattgtcct
     3841 tctcctgcaa gagaattatt ttaattttcg cttaattcag cgaaaccaaa gtaaagtggt
     3901 caagccccgc gcatagggca agcttaggca gaagaaagga ctaaagcgcg ttgcgccagt
     3961 gctgctcacc tacgagcaag atagtggatg aggtacgaag atgggtaaat gttgaaaata
     4021 acgtcctgaa caaattgtcc ataatattac aattatccgc agtgttgctt ctcgtcatcg
     4081 cggatgataa ttgtcctcgg tagttgaaca cgcctgattt gtatcatagc ttaagaatta
     4141 actcaaaata ttttcacttc tttacctgag cggtttgatt ttcgttatga tgacggagcg
     4201 aaaaagacat tattattagc aaaggaagaa aaaacgggga caagcatggc aaagccgatc
     4261 atcacgctca atggcctaaa aatcgtcatt atgttgggaa tgctggtcat tattctctgc
     4321 ggtatccgtt ttgccgccga gatcatcgtg ccgtttattc tcgcattatt tattgctgtt
     4381 attcttaacc cgctggtgca acacatggtc cgctggcgag tgccgcgtgt actggcggtg
     4441 tcgattttga tgaccatcat cgtgatggcg atggtgttgc tgttagctta tctgggttcc
     4501 gcgctcaacg agttgacgcg gacgttaccg caatatcgca actctattat gacgccgctg
     4561 caagctcttg agccgttgtt gcaacgcgta gggattgacg tctcagttga ccaactggcg
     4621 cattacattg atccgaacgc ggcgatgacg ttgctcacca acttattgac gcagttatct
     4681 aatgccatgt catcaatatt tttattgctg ctgacggtgc tgtttatgct gctcgaagtg
     4741 ccacaattgc ccggaaaatt tcagcaaatg atggcgcgtc cggttgaagg gatggcggcg
     4801 attcaacgtg cgattgacag tgtttctcat tatctggtgc tgaaaacagc catcagcatc
     4861 atcaccggcc tggtcgcctg ggcgatgctc gccgcactcg atgttcgctt cgcttttgtc
     4921 tggggattgc tggcctttgc gcttaattac atccctaata ttggttcagt tctcgcggca
     4981 atccccccta tcgctcaggt actggtgttt aatggcttct acgaagcgtt gctggtgctg
     5041 gcgggatatc tgctgattaa tctggtcttc ggcaatattc tggagccgcg catcatggga
     5101 cgtgggctgg ggctttcgac attggtggta tttttgtcgt tgattttttg gggatggttg
     5161 ttaggaccgg tgggtatgct gctttccgtg ccgttgacaa ttattgtcaa aattgcgctt
     5221 gaacaaacag cgggaggtca aagcatcgcc gttctgttaa gcgatctcaa taaagagtga
     5281 cggcctccgc agaggccgtc agggttacag agctttcagg attgcatcca cgctggcttt
     5341 ggcgtcacca aacagcatgt gggtgttttc cttgaagaac agcgggtttt gcacaccagc
     5401 atagccagtg ttcatcgaac gtttaaagac aatcacgttc tgcgctttcc acacttccag
     5461 cacaggcata ccagcaatag gactcttcgg atcatcctgc gccgccgggt taaccgtatc
     5521 gttagcacca atcaccagta cggtatcggt atcagcaaag tcatcgttga tctcgtccat
     5581 ttccagcacg atgtcatacg gtacttttgc ttcagccagc aatacgttca tatgtccagg
     5641 caaacgcccc gcaaccgggt ggataccgaa acgcacatta ataccacgag cgcgcaattt
     5701 ctcagtaatt tcagcgacag gatattgcgc ctgcgcaact gccatgccgt accccggagt
     5761 aatgatcact gaatgggagt ttttcagcag ttccgctgtc tcttctgcag tgatttcgcg
     5821 gtgctcgccc acttcctgat catcaccagt agaagagccg tcggtgccga aaccacccgc
     5881 aataacgctg ataaaggaac ggttcatcgc cttacacata atgtaagaaa ggatagcccc
     5941 cgaagaaccg accagcgcac cggtcacaat cagcaggtcg ttgctgagca taaagcccgc
     6001 agccgcagcc gcccagccgg agtacgagtt cagcatcgac accaccactg gcatatctgc
     6061 accaccgatg gaggcgacta aatgccagcc gaataccagc gcaatggcgg tcattatcag
     6121 caatgccagc acttgcaggc cgacgctatc cgtgcgaaca aatacaatca gcagcaggaa
     6181 ggaaacgacc agagccgcca ggttcatttt gtgacggttt ggcagcatca acggtttaga
     6241 cgaaatcttg ccacacagtt tgccgaacgc caccaccgaa cccgtgaacg ttaccgcccc
     6301 gatgaagata ccgaggaaca cttccgtcag gtgaatattg accagaatcg gtgccattcc
     6361 cgcgtcatga tgcagatagc tgttaaagcc aaccagcact gccgccagac ccacgaagct
     6421 atgcaggatc gccaccagtt ctggcatttc ggtcatttca actttcttcg ccagacggat
     6481 accaattgcc ccaccaatga ccatcgccag caagatccag ccaacattac ccgtatccgg
     6541 tccaaaaatg gttgcgatta acgcaatcgc catcccggcg ataccgaagt tgttaccctg
     6601 gcgagacgtt tcatgtttcg aaagaccggc cagactgaag ataaacagga tcgcggcaac
     6661 aatgtatgca gctgtaacta atcctccaga catatgttac cccttaattt ttgcggaaca
     6721 ttttcagcat gcgctgagtc acggtgaagc caccgaaaat attaatgctg gctataagca
     6781 ccgcgataaa actaaggaag ctaacccagc cgccctggcc aatctgcaac agtgctccga
     6841 caacaataat ccctgaaatc gcgttggtga ccgacatcaa cggtgtatgc agcgcatgcg
     6901 atacattcca caccacgtaa taaccgacaa cgcaggccag cgcgaaaacg gtgaagtgcc
     6961 caagaaactc tttcggcgca acgcttgcca gccaaccgaa aaggatgatt gccagcgcca
     7021 tcaatgcgta tttacgccac ggtgagcagg cacatttttc ctcagttttc acttccggtg
     7081 ccgctttttg tgccgcctgc ggctgagctg atacctgaat cggcggtgcc ggccaggtaa
     7141 tttcgcccgc acggatcacg gtcacgccgc gaatcaccac atcatcaaaa tcaacagtga
     7201 tattgccgtc tttctctttg cacaacagtt tcagcagatt aacgaggtta gtaccgtaaa
     7261 gctgtgagga ttgcgtcggc agacggcccg gaagatcggt ataaccaatc actttgacac
     7321 cattttccgt agtgaagatt tcacccggca cggtgtatgc acagttgccg ccgttttggg
     7381 ctgccaggtc gacaatcaca ctgcccgcct tcatggagtc aaccatttca cgggtaatta
     7441 gcttcggcgc tggtttgcct ggaataagcg cggtggtgac aatgatatcg acctcttttg
     7501 cctgggcggc aaagagttcc atttccgctt tgatgaacgc gtccgacatc actttggcat
     7561 agccatcgcc gctgcccgct tcctctttaa aatccagctc gaggaattcc gcgcccatac
     7621 tttgaacttg ttctttcact tccgggcggg tgtcgaatgc acgcacaatc gcgccgagac
     7681 tgtttgctgc gccaatggcg gccagacctg caacacccgc accaatcacc atcacttttg
     7741 ccggtggcac tttcccggcc gcagtaattt gcccggtaaa gaagcgccca aattcatgtg
     7801 ccgcttcaac aatggcgcga taaccggcga tgttcgccat cgagcttagt gcgtccagcg
     7861 attgtgcgcg tgagatacgc ggcacagagt ccatcgccat cacggtcacg ttacgttccg
     7921 caagtttttg cattaattcc ggattctgcg caggccagat aaaactcacc agcgttgtcc
     7981 caggattcag taacgcaatt tcatcatcta acggcgcatt gaccttcaga atgatctctg
     8041 actgccagac gctattccct tctacaattt cagcgcccgc ttgcacaaac gctttatcgt
     8101 caaaacttgc cagttgaccc gcgccgctct ctaccgcgac ggtaaaaccc agtttcagca
     8161 gctgttccac tgtttttggc gttgctgcaa cacgggtttc attggttaac cgttctcttg
     8221 gtatgccaat tcgcatgata ttcccttcca tcggttttat tgatgatggt ttgcctgtgt
     8281 caggagccac acaagccgct catgtacgag ctaaatgtta ctccgttaaa ataaattagt
     8341 aacaaacgcc ctataacgta ctgaaaatta tgcctgtgat ctagcgccaa aaatcagtat
     8401 ttcggcgtga actcgcaaaa tattaacgat tcagccgtga tagtgggata aacaccttag
     8461 aacgccggat aaagactgat aattgtcttc gacggtcggg taaaacgaga catcgccccg
     8521 gcacgcatca ctacttaaca ttaaattaac ttatacaatt cagttgcttc agtagtaatg
     8581 atgctgatac ggctgttttt taagcataga cggtcatttg agcaggatta aaattggctt
     8641 aaggaatgtg atatgaaaaa tgacgcagac agttacaccg tttaaatgca ataatcagcc
     8701 acgtttctcg ttaataacaa taccagtact tggtttgcgc aaggcgaagg attattttta
     8761 tgaagcttaa gaacaccctc ctggcgtcgg cactgctttc tgctacggca ttctccgtta
     8821 acgcagcaac agaactgaca ccggagcaag cggcagcggt taaacctttt gaccgtgtag
     8881 tggttaccgg tcgttttaat gctattggcg aagcggtgaa agccgtttct cgtcgcgcag
     8941 ataaagaagg tgccgcctct ttttatgttg tcgacacttc tgattttggt aacagcggta
     9001 actggcgtgt ggtcgctgac ctctataaag ccgatgctga aaaagcagaa gaaacaagta
     9061 atcgcgtaat taacggtgtt gtcgaactgc cgaaagatca ggctgttctg attgaaccgt
     9121 ttgacacggt caccgtccag ggcttctatc gtagccagcc agaagtcaat gatgccatca
     9181 ccaaagcagc aaaagcgaaa ggtgcctact ctttctacat cgttcgtcaa atcgatgcca
     9241 accagggcgg caaccagcgt attactgcat tcatctataa aaaagatgct aagaaacgta
     9301 tcgtccagag cccggatgtg atcccggcag attccgaagc aggacgtgca gctctggctg
     9361 ccggtggcga agccgcgaag aaagttgaga tcccgggtgt tgcgactacc gcatcaccaa
     9421 gttctgaagt cggtcgcttc tttgaaaccc agtcatcaaa aggcgggcgt tacaccgtca
     9481 cgctcccgga tggcactaaa gtcgaagaac tgaacaaagc gaccgcagcg atgatggtcc
     9541 cgttcgacag catcaaattc tctggcaact acggcaacat gaccgaagtc tcctatcagg
     9601 ttgcgaaacg tgccgcgaag aaaggtgcta agtattacca catcacccgc cagtggcagg
     9661 aacgtggtaa taacctgacc gtcagcgcag atctgtataa ataactattc agttactcct
     9721 ataaggcggc ttgatgccgc ctttcgcatt tttagtcaca aatttccccg cgaccattgc
     9781 atgcagacac atctcttcgt aaaatcccgc gcttcagtgg caaactccat ttttatgcgt
     9841 tttgccctaa tatttattct tcttatcacg ttttaatcac tggatatcga tggaaaagaa
     9901 actgggactg agcgcactca ccgcgctggt attaagctca atgctgggcg cgggtgtttt
     9961 cagtctgccg caaaatatgg cggcagttgc cagcccggca gcactgctca tcggctgggg
    10021 tattactggc gctgacattt tattgctggc ctttgccatg ctgatcctca cgcgcattcg
    10081 tcccgaactt gacggcggta tctttaccta tgcccgcgaa gggtttggcg agctaatcgg
    10141 cttttgttcc gcatggggat actggctgtg cgcagtcatc gccaacgtct cctatctggt
    10201 gatcgttttt tccgcgttaa gcttttttac ggacacgccg gaattgcgcc tgttcggcga
    10261 tggtaatacc tggcaatcga tagtcggtgc atcggcttta ttgtggattg ttcacttttt
    10321 gatcctgcgc ggggtgcaaa ccgctgccag cattaacctg gtggcgacac tggcaaaatt
    10381 gttgccgctg ggtctgtttg ttgtgctggc gatgatgatg ttcaaactgg ataccttcaa
    10441 gctcgacttc accggacttg cacttggcgt acccgtgtgg gaacaggtga aaaacaccat
    10501 gctgatcacc ctgtgggttt tcattggtgt ggaaggtgcc gtggttgttt ctgcgcgtgc
    10561 gcgtaataaa cgtgatgttg gcaaagcgac actgctggcg gttctctccg ctctgggcgt
    10621 ttacttgctg gtaacgctgc tttcgctggg cgtggtggca cgtcctgaac tggctgaaat
    10681 tcgtaacccg tcaatggccg gtctgatggt ggaaatgatg ggaccatggg gcgaaatcat
    10741 catcgctgcc ggtttgatcg tttccgtttg cggcgcgtac ttaagctgga ccatcatggc
    10801 ggcagaagtt ccgttcctgg ctgccactca taaagcattc ccacgcattt ttgcgcgcca
    10861 gaacgcacaa gctgcgccat ccgcctcgct gtggttgacc aacatctgcg tgcaaatttg
    10921 tctggtactc atctggctga ccggttccga ttacaacacg ttgctgacca tcgcctcaga
    10981 aatgattctg gtgccctatt tcctggtcgg cgcgttcttg ctgaaaatcg caacccgtcc
    11041 actgcataaa gcggtaggtg tcggtgcctg catttatggc ttatggttat tgtatgcctc
    11101 tggtccaatg cacttgttgt tatccgttgt tctgtatgcg ccggggctgc tggttttcct
    11161 gtatgcgcgt aaaacgcata cacatgataa cgtactgaac cgccaggaaa tggtgctaat
    11221 tggcatgctg ttaattgcct cagttccggc gacctggatg ctggtgggat aacgttcatc
    11281 ccatcgttgg ttagcaaagg agataacgat gggtaaagcc cagcccttgc caatattaat
    11341 tactggcggt ggtcgtcgca tcggcctcgc cctcgcatgg catttcatta atcaaaagca
    11401 accggtgatt gtcagctatc ggacacacta tccagccatt gatggactga ttaatgcagg
    11461 tgcgcagtgt attcaggctg atttttcgac caacgacggt gtgatggcgt ttgccgatga
    11521 agtactaaaa agcacccatg gtctgcgtgc tattttgcat aacgccagtg cgtggatggc
    11581 ggaaaaaccg ggtgcgccac tggccgacgt actggcttgc atgatgcaga tccacgttaa
    11641 taccccatac ctgctcaacc atgcgctgga aagattactg cgtgggcacg gacacgccgc
    11701 cagcgatatc attcacttta ccgattatgt ggtggagcgc ggtagcgaca aacatgttgc
    11761 gtatgctgca agcaaagcgg cactggataa tatgacccgc tcgtttgccc gcaagctggc
    11821 accggaagtg aaagtgaatt ctattgcgcc atcgctgatc ctgtttaatg aacatgatga
    11881 tgccgaatat cgacaacagg cgctgaataa atcactgatg aaaaccgcgc ctggcgagaa
    11941 agaagtgatc gacctggtcg attacttact taccagttgc tttgtcaccg gacgcagttt
    12001 cccacttgat ggcggtcgtc atctgcgtta atgcagcttt atccagcata taatcaacac
    12061 ccatgcgctt atcccccaac acgcaacaga accaacaaaa gcggcgggca agcgcatcat
    12121 tccggtgaaa taccacagcg acaccaggta aacaaaatag ggaataatcg accacatact
    12181 aaaaatgatg gttgcgcgta aggcttcaat gccgcgttcg ctggcaacaa tataatgcgc
    12241 gataagcgca aaggtcggga aaagtggaat cagcccggcg atataataat ttttcgtttt
    12301 tgctaaaaca ccaatcaaca gcactaccag cgcaccgagc gcggctttga ttaccagccc
    12361 cattttttta ccttaacact tccataacaa gtcatcagta gaatacctga tgaaaacttg
    12421 tttagaaacg attgatagta agtaaaaaca gcgcggtgta ttgtgacgtt tttatatcta
    12481 ccgtgaataa tatgaacact atcgtatttg tggaagatga tgcggaagtc ggttcactga
    12541 ttgccgcgta cctggcaaaa catgatatgc aggttaccgt agagccgcgc ggcgaccagg
    12601 ccgaagaaac cattttgcga gaaaatccgg atttggtgtt actcgacatc atgctaccag
    12661 gcaaggacgg catgaccatt tgtcgtgatt tacgcgcaaa gtggtctgga ccgattgttc
    12721 ttctaacctc tctcgatagc gatatgaacc acatcctggc actggaaatg ggtgcctgcg
    12781 actatattct caaaacgacg ccccctgctg ttttgctagc gcgtttacgt ttgcatttgc
    12841 gtcagaatga gcaagccaca ctgaccaaag gtcttcagga aacgtctctg actccctaca
    12901 aagccctgca tttcggcacg ttgaccatcg atcccatcaa ccgcgtagtc accctggcta
    12961 acactgaaat ctcgctctcg acagctgatt tcgaattatt gtgggaatta gctacccatg
    13021 ccgggcaaat catggaccgc gatgcattgc tgaaaaattt acgcggcgtc agttatgacg
    13081 gactggatcg tagcgtggac gtggctattt cgcggttaag aaaaaaactg ctcgataacg
    13141 ccgcagaacc ttatcgcatt aaaactgtgc gtaacaaagg ctatcttttt gcgcctcatg
    13201 catgggaata agcgatgaaa aaactgttta tccagtttta cctgttattg tttgtctgct
    13261 tccttgtgat gtctctgctg gttgggctgg tgtacaaatt taccgccgaa cgcgcgggca
    13321 aacagtcgct ggatgatttg atgaacagtt cgctgtatct gatgcgtagc gaattgcgtg
    13381 agatcccccc acacgattgg ggtaagacgc tgaaggagat ggatttaaat ctctctttcg
    13441 atctgcgtgt cgagccactg agtaaatacc atcttgatga tatttccatg caccgactgc
    13501 gtggcggcga aattgtcgcc ctggacgatc agtacacgtt tttgcagcgt atcccgcgca
    13561 gccactacgt gctggcagtt ggtcctgttc cttatcttta ttatctccat cagatgcgat
    13621 tgctggatat cgccctgatc gcttttattg ctatttccct cgcctttccg gtgtttatct
    13681 ggatgcgtcc gcattggcag gatatgttaa aactggaagc agcggcgcaa cgatttggcg
    13741 atgggcatct caatgagcgt atccactttg atgagggttc gagctttgaa cgacttggta
    13801 tcgcatttaa ccagatggcg gacaatatca acgccttgat tgccagcaaa aaacagctta
    13861 ttgacggtat cgctcacgaa ctgcgaacac cgttagtgcg cctgcgttat cgactggaga
    13921 tgagcgataa cctgagcgcc gccgaatccc aggcgttgaa tcgtgatatc agtcaacttg
    13981 aagctttaat tgaagagctg ctgacttatg cccgactcga tcgcccacaa aacgagcttc
    14041 atcttagcga accagacctg ccgttgtggc tgtcaacgca tctggcagat attcaggcag
    14101 taacgcccga taaaacggta cggataaaaa cgctcgtgca aggccattat gcggcgttgg
    14161 atatgcgctt aatggagcgc gtgctggata atttgctcaa taacgccctg cggtactgcc
    14221 attcaacggt tgaaaccagc ctgctactgt cggggaatag agcgacatta attgttgagg
    14281 atgatggccc agggattgcc ccagaaaacc gcgaacatat ctttgaacct tttgttcgcc
    14341 tcgatcccag ccgggatcgc tcaaccggcg gctgcgggct ggggctggca attgtccact
    14401 ctatagcact ggcaatgggc ggtacggtta attgtgacac cagcgaactg ggtggtgctc
    14461 gcttctcgtt tagctggccg ttatggcata acatcccgca atttacctct gcctgacact
    14521 acgcgcacga tggtcaagtc accaccattg tgctataaat taaggatgtt gtaactaaag
    14581 tggttaatat tatggcgcgt tacgatctcg tagaccgact caacactacc tttcgccaga
    14641 tggaacaaga gctggctgca tttgccgctc atcttgagca acacaagcta ttggttgccc
    14701 gcgtgttctc tttgccggag gtaaaaaaag aggatgagca taatccgctt aatcgtattg
    14761 aggtaaaaca acatctcggc aacgacgcgc agtcgctggc gttgcgtcat ttccgccatt
    14821 tatttattca acaacagtcc gaaaatcgca gcagcaaggc cgctgtccgt ctgcctggcg
    14881 tgttgtgtta ccaggtcgat aacctttcgc aagcagcgtt ggtcagtcat attcagcaca
    14941 tcaataaact caagaccacg ttcgagcata tcgtcacggt tgaatcagaa ctccccaccg
    15001 cggcacgttt tgaatgggtg catcgtcatt tgccggggct gatcaccctt aatgcttacc
    15061 gcacgctcac cgttctgcac gaccccgcca ctttacgctt tggttgggct aataaacata
    15121 tcattaagaa tttgcatcgt gatgaagtcc tggcacagct ggaaaaaagc ctgaaatcac
    15181 cacgcagtgt cgcaccgtgg acgcgcgagg agtggcaaag aaaactggag cgagagtatc
    15241 aggatatcgc tgccctgcca cagaacgcga agttaaaaat caaacgtccg gtgaaggtgc
    15301 agccgattgc ccgcgtctgg tacaaaggag atcaaaaaca agtgcaacac gcctgcccta
    15361 caccgctgat tgcactgatt aatcgggata atggtgcggg cgtgccagac gttggtgagt
    15421 tgttaaatta cgatgccgac aatgtgcagc accgttataa accacaggcg cagccgcttc
    15481 gtttgatcat tccacggctg cacctgtatg ttgcagatta acgcccggct ttcatactgc
    15541 cgaccatctg ttctggccgt acccagctgt caaactcggc ttcgctaaga taccccagcg
    15601 caagggccgc agcttttaag gtcagccctt ctttatgcgc ttttttggcg atctccgcgg
    15661 ctttgtcata accaatgtgg gtgttaagcg cagtcaccag catcagcgat tcattgagta
    15721 attgattgat tcgctcacga ttcggttcaa tacccactgc gcagtgtttg ttaaaacttt
    15781 ccatgccatc tgccagcaag cgcaccgatt gcaggaaatt atggatcacc atcggacgga
    15841 agacgttcag ttcaaagtta ccggaagcgc cacccatgtt gatcgccacg tcgttcccca
    15901 ttacctgaca gcagagcatg gttaatgctt cacactgggt tgggttcact ttccccggca
    15961 tgattgagct gcccggctca ttttccggga ttgagatttc accaattccg cagcgcgggc
    16021 cagaggccag ccagcggaca tcattggcga ttttcatcag tgacgcagcc aaccctttca
    16081 acgcgccgtg cgcctgaacc agggcatcac aggtcgcaag cgcttcaaat ttgttcggcg
    16141 cggtaacaaa cggtgcacag gtaatgactg ccagttcatc tgctacgcga cgcgcatact
    16201 ccggatgggt atttagtcca gtacccaccg ctgtaccgcc aagagccagt tccgctacgt
    16261 gaggcaggct gtattcgata tgtttgagat tatgctcgag catcgctacc cagccggaaa
    16321 tctcctgccc tagtgttagc ggcgtggcgt cctgcaagtg ggttcgaccg attttgacga
    16381 tatcggcaaa tgcacgcgat ttttcactca gtgtctgtgt cagggtttta agctgcggaa
    16441 tgagttgctt gcgcagcgcc agcagcgccg caacgtgcat cgccgtcgga aagacatcgt
    16501 tggaactttg gcttttgttc acgtcgtcgt tagggtgaac tttacgttcc atcccgcgca
    16561 cgccgccgag taattcactg gcccggttag ccagcacttc gttcatgttc atgttacttt
    16621 gcgtgccgga gccggtctgc cagatagcca gcgggaattc gtcgtcatgc tgtcctgcca
    16681 gtacttcatc cgccgcctgc cgaatggcgc tcgctttctc ttcagacaac aagcctaaat
    16741 cttcattaac ttttgccgct gcgcgcttgg ttagcgccag cgcatgaatc agtgaggtgg
    16801 gcattttctc cgtcgaaatg cggaaatgct ccagcgagcg ttgagtttgt gcgccccaca
    16861 gcttatctgc cgggacatca atcgccccca tcgaatcttt ttcgctgcgt actgtattca
    16921 tggcctgctc ctcacctgtt taattttttc tttctgtttt gctttcgtta agcaactttt
    16981 agctcactca ttatttacta tttgataaca aatgtttggt ctttcgtgcc atgtaaaaaa
    17041 accgccccga agggcggctc tgtttatttc acacagcggg tgcattgtgt gagttgtatc
    17101 tgctggaaga agtcatttcc tttatcatcc acaaggataa acgccgggaa atcttccact
    17161 tcaattttcc agatggcttc cattcccagt tccggatatt caacacattc caggctctta
    17221 atacttccct gcgccaatac agcggccgga ccaccgatac tgccgaggta gaagccgccg
    17281 tgttttttac aggcatccgt cacctgctgg ctgcggttgc ctttcgccag catgatcata
    17341 cttccgccct gcgcttgcag ttgatcgaca taagaatcca tccgtccggc ggtcgttggg
    17401 ccaagagaac cggaagcata accttccggc gttttggccg gacccgcgta gtaaatcgga
    17461 tgatctttaa tgtactgcgg cagcccttca ccattatcca tccgctcttt cagtttggcg
    17521 tgagcaatat cacgaccgac gataatcgtg ccgttaagcg ataagcgtgt agaaacggga
    17581 tactgcgaca actgtgcgag gatctctttc atcggacggt taaggtcaac gcgcaccgct
    17641 tcgccttctc ccgctttgcg cagctcttcc gggatatatt tgcctggatt atgttccagt
    17701 ttttcgatcc agatcccctg acggttgatc ttcgctttga tattacggtc agcagagcag
    17761 gagacgccca taccgaccgg gcaggatgcg ccgtgacgtg gcaggcgaat cacgcggatg
    17821 tcgtgagcga agtatttacc accaaactgc gcacccagac cgagattttg cgcttcgatc
    17881 agcaattctt tttccagttc cacatcgcgg aatgcctgac cgtgctcatt cccttccgtt
    17941 ggcagttcgt catagtattt agcggaagcc agtttcactg ttttaaggtt cgtttctgca
    18001 gaagttccac caataacgaa cgcaatatga tacggaggac aggccgccgt acccagcgtg
    18061 cgcatcttct caaccaggta atttttcagt ttccccggcg ttagtaacgc tttggtttcc
    18121 tgatagagat acgtcttgtt tgccgaaccg ccacctttgg cgatacagag gaatttgtac
    18181 tcgtcgccat caacggcata aagatcgatc tgcgctggta gattggtgcc ggtattcacc
    18241 tctttataca tatccagcgg cgcgttttgc gagtagcgca gattatcttc gatataagtg
    18301 ttatagacac cgcgtgccag cgccgcttca tcaccgccac cggtccatac acgctgccct
    18361 tttttaccaa caataatcgc ggtgccggta tcctgacagg ttggcagaac gcctttcgcc
    18421 gcgatgtcgg agttgcgcag gaattgcagc gccacatatt tatcattttc gctggcttcc
    18481 gggtcacgca gaatgtcggc cacctgttgt tggtgagccg gacgcagcat aaatgacgca
    18541 tcatgaaacg cctgacgcgc caacagagtt aacgcttcgg gtgcgacttt caaaatctcc
    18601 tgcccttcaa attcagatac gctaacgtgt tcgctggtta gcaggtaata ctcagtatca
    18661 tcttttttga gtgggaaagg agcctgataa tgaaagggtt tgtttgacat tattctctca
    18721 cttactgcct ggtttggtta tgctctgggc gggtgttccg ttgccctgtt aaaagcgagt
    18781 aacaatatcc tacacacttt tttaacaaaa actgagacta gtacgacttt ttgcggctcc
    18841 aggttacttc ccgtaggatt cttgctttaa tagcgggatt aatttccaca ttaaaacagg
    18901 gattgatcat gcaaaaactc attaactcag tgcaaaacta tgcctggggc agcaaaacgg
    18961 cgttgactga actttatggt atggaaaatc cgtccagcca gccgatggcc gagctgtgga
    19021 tgggcgcaca tccgaaaagc agttcacgag tgcagaatgc cgccggagat atcgtttcac
    19081 tgcgtgatgt gattgagagt gataaatcga ctctgctcgg agaggccgtt gccaaacgct
    19141 ttggcgaact gcctttcctg ttcaaagtat tatgcgcagc acagccgctc tccattcagg
    19201 ttcatccaaa caaacacaat tctgaaatcg gttttgccaa agaaaatgcc gcaggtatcc
    19261 cgatggatgc cgccgagcgt aactataaag atcctaacca caagccggag ctggtttttg
    19321 cgctgacgcc tttccttgcg atgaacgcgt ttcgtgaatt ttccgagatt gtctccctac
    19381 tccagccggt cgcaggtgca catccggcga ttgctcactt tttacaacag ccaaatgctg
    19441 aacgtttaag cgaactgttc gccagcctgt tgaatatgca gggtgaagaa aaatcccgcg
    19501 cgctggcgat tttaaaatcg gccctcgata gccagcaggg tgaaccgtgg caaacgattc
    19561 gtttaatttc tgaattttac ccggaagaca gcggtctgtt ctccccgcta ttgctgaatg
    19621 tggtgaaatt gaatcctggc gaagcgatgt tcctgttcgc tgaaacaccg cacgcttacc
    19681 tgcaaggcgt ggcgcttgaa gtgatggcga actccgataa cgtgctgcgt gcgggtctga
    19741 cgcctaaata cattgatatt ccggaactgg ttgccaatgt gaagttcgaa gccaaaccgg
    19801 ctaaccagtt gttgacccag ccggtgaaac aaggtgcaga actggacttc tcgattcctg
    19861 tggatgattt tgccttctcg ctgcatgatc ttagtgataa agaaaccacc attagccagc
    19921 aaagtgccgc cattttgttc tgcgtcgaag gcgatgcaac gctgtgtcaa ggttctcagc
    19981 agttacagct taaaccgggt gaatcagcgt ttattgccgc caacgaatca ccggtgactg
    20041 tcaaaggcca cggccgttta gcgcgtgttt acaacaagct gtaagagctt actgaaaaaa
    20101 ttaacatctc ttgctaagct tattaaaggc ttataacacc ttcaggcggc cagtccgcct
    20161 gatttcattt tatggataat cattatgaat aaatcgctgg tagcggtagg cgtcattgtt
    20221 gcgctaggcg tagtctggac aggcggcgca tggtatacag gcaagaagat tgaaacccat
    20281 ctcgaagaca tggtcgcgca ggcgaacgcg caactcaaac tgacagctcc tgaatccaac
    20341 ctggaagtga gttatcaaaa ctatcatcgc ggcgtattca gcagccagtt gcaactgttg
    20401 gtgaaaccca ttgccgggaa agaaaatccg tggattaaaa gcggtcagag cgtcatcttc
    20461 aacgaatcgg ttgatcatgg tcccttcccc cttgcccagc ttaaaaaact gaacctgatc
    20521 ccgtcgatgg catcaattca aaccacgctg gtcaataacg aagtaagcaa accactgttt
    20581 gatatggcaa aaggtgaaac gccttttgag attaactccc gcattggtta cagcggtgat
    20641 tccagttccg atatttcgct caatccactg aattacgagc aaaaggatga aaaagtcgcc
    20701 tttagcggcg gcgagttcca gttaaatgct gacagagacg gcaaagctat ctccctttcc
    20761 ggggaagcgc aaagtggtcg gatagacgca gttaacgaat acaatcagaa agtgcagttg
    20821 acctttaata atctgaaaac cgacggttcc agcacgctgg caagttttgg tgagcgcgta
    20881 ggaaaccaaa aactgtcact ggaaaaaatg accatttcag tggaaggcaa agaactggca
    20941 ctgctggaag gcatggagat cagcggtaaa tcggatctgg tcaatgatgg taaaacgatc
    21001 aatagccaac tggattactc gctaaacagc ctgaaggtac agaatcagga tctgggcagc
    21061 ggcaagctga ctttaaaagt cggccaaatt gatggcgaag cctggcatca gtttagccag
    21121 caatataacg cgcaaactca ggcgctgctg gcacagccag aaattgccaa caatcccgaa
    21181 ctttatcagg agaaagtgac ggaagccttc tttagcgccc tgccgctgat gctgaaaggc
    21241 gatccggtga ttactattgc gccgctaagc tggaaaaaca gtcagggtga aagtgcgctg
    21301 aatctgtcgc tgttcctgaa agatccggca acgactaaag aagcgccgca aacgctggcg
    21361 caggaagtag atcgttcggt taaatctctg gatgcgaaac tgaccattcc ggtggatatg
    21421 gcaactgagt ttatgactca ggtagcgaag ctggaaggtt atcaggaaga tcaagcgaaa
    21481 aaactggcga aacagcaagt tgaaggtgca tcagcaatgg ggcagatgtt ccgtctgacc
    21541 accttgcagg acaataccat caccaccagc ctgcagtatg ctaacggtca gatcacgtta
    21601 aacgggcaga aaatgccact ggaagatttt gttgggatgt ttgcgatgcc ggcattaaac
    21661 gttccggctg tacccgctat tccgcagcag taattcacag ccaaaacgcc ggaggttttt
    21721 ctctccggcg ttatttctta cttcagcata aagtcatact gaatactcat tttcacatcc
    21781 cagtaattca ggcgatcgcc cagcgcactg ccatcgcgct tttcccgccc tttatcagca
    21841 gcaagaaaga aattcatccc ttgtaacggc ccttcgctga cccgatagtt cataccacct
    21901 aagatacccg tgccttcaat ccgcacatct cgtttactgg attcatagcc gcgagtcaca
    21961 atcggtgcaa atgtcagtgt aaattgcggc gttaaacgat agttgacgcc caattgccag
    22021 gactgcattc cttcacggtt acgatcaaga cttaatgaaa agggatagcc aatgtcggta
    22081 tccaccacgg caccattcat tgcatggcgc aaataaccac cactgccaaa tacgcttccc
    22141 cacggcgcat tccaggtcaa ttgtccacta accaacgccg tttcattggg ctggctggta
    22201 ttacgactta aaccttcgag ctgcgcgtaa aagccgagca actccccttt caacgtataa
    22261 ttcgggtcga gcggtatgct tttctcgaga atgctttgta tctgattgga aacatctttc
    22321 tgtaccgatg cccccagaga atagcgactg gtatcatcat gccaactggc ggctaaaaaa
    22381 gaacgtgcgc gatgtttatc gtattttccc gtctggctgt tatacacccc ttcatcacga
    22441 ataccggttg ttgaatcggt ccataaaaat actcgtgaac gccacattcc ccacagatca
    22501 atatttggcg ttaacgccca ataacctaat gctccttcag aaactgtcgg taatgtacgt
    22561 tgcgaactgc cagccattaa cggaatacga taaggtaagc taccgtaact ataatccgtc
    22621 ccgaagcgac ctaatttaag caggctattt tcactgggtt ttatttttac tgcccccagg
    22681 gcaaagccaa aacttttatc accatcaaga taccaccggg tactcatatc agcacgagca
    22741 cctaatttat aaacataata cgctccgcct tcaacgccaa taatattgga ataataacca
    22801 ctattgaaat cgagcaatcc tccctgtacc catgcgtaaa tatctcttcc cgcgccagca
    22861 ctgggcttat ccgccctgcg caattcgttt ttcagtctga tgcgtagtcc cgcttcatcg
    22921 tcagccagtt gcgccgcgta agcagaggta aggccagagg cagccgtcag gcaaataacg
    22981 gccatggcca ctatttttct catagttgca cctttgatgt tctgattatt tttattgaat
    23041 attaattagt gatatcgctg attaattgct gctgcatttt tttacgatta tcaatttcaa
    23101 ccacgatttc tttgaatttt ttatccgtga gcggataaaa ccagataata acgaatgcca
    23161 gtagcataaa tccgcaaggt actaaggcaa ttgatgtgcg gatgcccata ataacttccg
    23221 gcgtttgcac
//
LOCUS       DAFNBJ010000074        22603 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000074 DAFNBJ010000000
VERSION     DAFNBJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.074"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(942..1127)
                     /locus_tag="L2X49_002679"
     CDS             complement(942..1127)
                     /locus_tag="L2X49_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797748.1"
                     /translation="MDRKSNSDSYQRWMIEKADKRERTQISMSFVKMKDVLFNVLAIG
                     TVLAALVVIFYEVQSSL"
     gene            complement(1085..1297)
                     /locus_tag="L2X49_002680"
     CDS             complement(1085..1297)
                     /locus_tag="L2X49_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797749.1"
                     /translation="MVTQSLADFGRYSYKPRPAFQDWIERLKTGERLPRGRYFVGKKP
                     GGRLVLIDELGGTWTEKVTVIHTNAG"
     gene            complement(1360..1635)
                     /locus_tag="L2X49_002681"
     CDS             complement(1360..1635)
                     /locus_tag="L2X49_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797750.1"
                     /translation="MHTTNAIILCLVSYPCIVAWVIYKNRFVDYLFLTLLACLGSIFS
                     VVLYKQPDYSLTIYQAVAVGYAAVIMPTVLVLSVVNIFMGKAERTCK"
     gene            complement(1653..1907)
                     /locus_tag="L2X49_002682"
     CDS             complement(1653..1907)
                     /locus_tag="L2X49_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797751.1"
                     /translation="MGHYTIRTNDDEDQAIKKAQEATGQASASKTFMTAILELQRNRD
                     EMAQLRRELAQEKARSQELVSSVKQFRSSLNNLFDLADNP"
     gene            complement(2017..2838)
                     /locus_tag="L2X49_002683"
     CDS             complement(2017..2838)
                     /locus_tag="L2X49_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HBN4797752.1"
                     /translation="MTRATERAYSELQQAFDFYNQRLFDGELPDCLITFQRGKNTMGY
                     FSYRRFVAADGSGRMIDEIALNPEYFPVYPLIEVMQTLVHEQCHMWQYHYGNPSRKTY
                     HNAQWAAKMESIGLMPSSTGRPGGAKVGQKINDYPIPGGRFQRVTLELFQGQFALSWF
                     DRFPVQVEQQKDMTAVIEQWRETLALAHQNAEAGIDIEAVLSMALLPSVSPQNGSHSD
                     DMAGSNDNDLSVAAFEDKPKRNKVKYQCRGCGAAVWGKAGLNIECGDCELAFIEN"
     gene            complement(2835..3014)
                     /locus_tag="L2X49_002684"
     CDS             complement(2835..3014)
                     /locus_tag="L2X49_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797753.1"
                     /translation="MTEKITDEELVDLLEALKRAHGMGVCSKAVKLAQRCADVFPAIV
                     AELQEYRNAAKRTSA"
     gene            complement(3031..3297)
                     /locus_tag="L2X49_002685"
     CDS             complement(3031..3297)
                     /locus_tag="L2X49_002685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797754.1"
                     /translation="MISIAFASYILLALVVHLTLKQASYNGKVSSPAVNHACQQTSFL
                     QPVWPLIFASNITRSMPALGVKLPAKKIAAIKGVVYIICIVRYR"
     gene            3287..3937
                     /locus_tag="L2X49_002686"
     CDS             3287..3937
                     /locus_tag="L2X49_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBN4797755.1"
                     /translation="MDIKPNSVIHLEPGETLPGYTNLKPVPDKYFDELKALINKLNTQ
                     SGNIYLKLKQMYAFLDRFNKEFVSTFTSCQKGCSSCCKMDVHLTALEATHIAQASKLT
                     ARDNPLTTGHESKCPFLSEKGTCSIYNYRPLLCRTYHVLTPPEMCNDLDAQVMQYGSQ
                     SANMGNHIYKTIAEWIYFQTYHCTGKLETKDIRDYFPYPREDIQRFLHHNPPRPFC"
     gene            complement(3975..4430)
                     /locus_tag="L2X49_002687"
     CDS             complement(3975..4430)
                     /locus_tag="L2X49_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HBN4797756.1"
                     /translation="MSENETFDATKKLLLNIRSVRVFARETSFEQLLEMQEKLNAVIE
                     ERREEAEREAAERAERERKRQELLQLIAGEGFSPEELLGLSEDAQKTRKKTLPKAPPK
                     YQFDENGETKYWSGRGRTPKPIDEALKAGRSLDEFLIKKDASSTAGDEQ"
     gene            complement(4442..6769)
                     /gene="topB"
                     /locus_tag="L2X49_002688"
     CDS             complement(4442..6769)
                     /gene="topB"
                     /locus_tag="L2X49_002688"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBN4797757.1"
                     /translation="MRLFIAEKPSLAKAIFEGLGGNPNTEKKNGYFEHGSDVVTWCYG
                     HMLELYDPQDYDDKYSNWRYSDLPIKAVYPPKYKIKPESEAQTRIILSLIDKADSIVH
                     SGDPDDEGCLLVDEILGYAGNKKPVQRLLVADLNLAPVQKSLSNLQPNDKFRGMTLSA
                     LARSLCDQGFGYNMTRGCTLKGREKGYDGVLNVGRVQSAVLGLVNMRTLANQNHTESF
                     YYDVFASLSMNGHQIKAKYQTTDDDQIDEKKRLISEAQAAHIAGRVTGKDAIVTIATT
                     KPENSKPPLPLNLSTLQQLCAKRFGYSAKDTLDVMQGLYETHKLLTYPRSDNRYLSDE
                     HYYQAGDIATAIAATVPELASAIVDMDKGQKHKAFNASKIEAHHAIIPTTKSGAGIQL
                     NEKERNVYNLVSVYFIGLFYPDAIRNKTKIHFDIKGDTFTATQSVLVQKGWEALGKDS
                     DDEDETEDAGTDGFDLSLLKFNDSGLCESADVDKKKTTPPRYFTESTLLAAMTSAAKF
                     IDDPALRKALEAKDEGSSDRGSIGTEATRAGILEKLAANTGLISIEKEKGYSELVWKT
                     TKQGQEFCAALPPEITKPDISALWAEKQSQIKAGELTVEEFIKENDEYVQGLIDELDR
                     NGISISSNATPCPVCNNGFLRKRKGQNGFFWGCSCYPECKTTFPDKDGKPDMEAKSRS
                     EGSMSRLEAPCPSCSKEIIIRPKGFFCSGCEFKIWSEVSGKKLTQNQVETLIKKGKTG
                     EIKGFTSNKTGKKFDAAIVLQDKTTGKLGFQFSKK"
     gene            complement(6773..8035)
                     /locus_tag="L2X49_002689"
     CDS             complement(6773..8035)
                     /locus_tag="L2X49_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN4797758.1"
                     /translation="MGKLTLVEQALRLHKEYYGEDNFGTVVKDCDYYPKVAEIFINRK
                     NRKIYSNLDIEPVASTVVHTGSSPVMAKAGRIKKLMFKDGSGAYRIHLGQNEVVHIIR
                     FILNSKVRMEYAVGTTKSMSMLHNLCEAGRAKLNRSLPRKGIWKIGCYDGVYYHGKAR
                     KEEIENALRFSVHPKFNELENDFNQFFSDIDFYTRYGQSGMRKVLFTGPPGTGKTTIA
                     KALGAKYQDKYVFVYADDYFKDVCYAAAQKKIPVIIIAEEVDELYRADAGTLSFLDGA
                     DTPRNLAGTYVIFSTNYPNRIDPRIRKRPGRIDRIISVGAFRTKAAAACAKMYLPDDI
                     NIDLKELGAVLDRTTPAEIKEIINIAIGMIRGTKNELTVDVIKNARAFLKGTLDLSVQ
                     EAEEDIEEREEIFKKNGAQPDYSSYLED"
     gene            complement(8118..8612)
                     /locus_tag="L2X49_002690"
     CDS             complement(8118..8612)
                     /locus_tag="L2X49_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBN4797759.1"
                     /translation="MRHFFNVLSFAVALLPATLCAAQIQGKVIRVLDGDTIEVKTLPA
                     KIVVYEVPIRVRLINIDAPEKKQPFGRWSTNQLKALLAGQSVTVSYTQTDRYGRVLGR
                     VVTANGTEANRQQVLKGAAWVYDRYNTDNSLPALQREAQTQKRGLWADSNPVPPWEWR
                     HKQN"
     gene            complement(8609..8935)
                     /locus_tag="L2X49_002691"
     CDS             complement(8609..8935)
                     /locus_tag="L2X49_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797760.1"
                     /translation="MKIINVPDARSVRIAGIEIIDSVKSVASVFTEILLFMVWFPAIV
                     YGLYRFVIVLWDASLKPQSLIEFINFMVELYSYVLPSLAVIAVALVVVLSIFKRLSAI
                     WEGRKQ"
     gene            complement(9021..9335)
                     /locus_tag="L2X49_002692"
     CDS             complement(9021..9335)
                     /locus_tag="L2X49_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797761.1"
                     /translation="MKKTVTTAVLLCAFAAGTAHAEEKADPNDPCAMVLCLAGKLDGS
                     SPAECDPMYKSFMSIRKKNKHGFLPDHTADARKKKLNECPAADAGTVSKIISSFGRLK
                     NF"
     gene            complement(9423..9815)
                     /locus_tag="L2X49_002693"
     CDS             complement(9423..9815)
                     /locus_tag="L2X49_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBN4797762.1"
                     /translation="MKKMLMVSVLFLSACSSPPEPPQVDWEQNPETVNTQLMDWQPTY
                     SVIKSDKVNSSWVKVIHNFRPENRLYDDAVFYSVAHSDSVIVETSNGTDFFTAKNWLR
                     ANGANGVIQYRYKMNCFSCRTTSVYLSR"
     gene            complement(9812..11647)
                     /locus_tag="L2X49_002694"
     CDS             complement(9812..11647)
                     /locus_tag="L2X49_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBN4797763.1"
                     /translation="MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKN
                     FDSMLLWRIITESNIRTDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTDVALYGDAKF
                     ASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPN
                     LLVRKHSLIALDPKQELWKITSKVREKLLGNKVYLLDPFNSKTHQFNPLFYIDLKEES
                     GAKDLLKLIEILFPSYGLTGAEAHFNNLAGQYWTGLAKLLHFFINYDPSWLGEFGLKP
                     VFSIGSVVDLYSNIDRELILSKREDLEGTKGLDENALYHLRDALTKIREYHETEDEQR
                     SSIDGSFRKKMSLFYLPTVRKCTDGNDFDLRQLRREDITVYVGVNAEDMSLAYDFLNL
                     FFNFVVEVTLRENPDFDPTLKHDCLMFLDEFPSIGYMPIIKKGSGYIAGFKLKLLTIY
                     QNISQLNEIYGVEGAKTLMSAHPCRIIYAVSEEDDAKKISEKLGYITTKSTGSSKTSG
                     RSTSKSSSESEAQRALVLPQELGTLDFKEEFIILKGENPVKAEKALYFLDPYFMDRLM
                     MVSPKLTELTASLNKTKKVLGVKGLKYPSKEKMLSVGELESEVLL"
     gene            complement(11650..12684)
                     /gene="virB11"
                     /locus_tag="L2X49_002695"
     CDS             complement(11650..12684)
                     /gene="virB11"
                     /locus_tag="L2X49_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HBN4797764.1"
                     /translation="MKDAENRHIVYEIIDEYFHHWLNEVDGLTEIAVNRPDELFVKVS
                     GKWQQHELKMDFKDCMSFAGAISDYHDGGSVTPEYPLRSVTLPGGERVQIVIPPATER
                     ETVSITIRKPSSVFIDHDTFVKQGFYSRLNQGGKFRDKEDELSSMFKDNCFEQFVPEC
                     LVKGKTMVFCAGTGAGKTTFANACLQYIPHHLRCISIEDTDEAKFRFHKNHVKLYYPS
                     EGESSTVSSASLLRSGFRMNPDRILMTEVRGAEAWDFLKGSSSGHAGNLTTVHESTPE
                     DAVLGLVQRCYMNPECQNLPYNIILRRVLSNVDVIMSIKYIDEEDNRFASGIYYRDIH
                     FQEYFEKLKE"
     gene            complement(12681..12878)
                     /locus_tag="L2X49_002696"
     CDS             complement(12681..12878)
                     /locus_tag="L2X49_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBN4797765.1"
                     /translation="MITLDIKEFSMLLGIRESEIYHHIRKGIPINGVPFPKPLKQIKT
                     HRFNYEEVMRFIEDLKGKGEL"
     gene            complement(12880..14094)
                     /locus_tag="L2X49_002697"
     CDS             complement(12880..14094)
                     /locus_tag="L2X49_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HBN4797766.1"
                     /translation="MSEQENKIPTAVEIEQALRERHQKEIEQNDKTDKGDEDDGKSVK
                     RLGIEKLKKSRKGLVIIVAAFLLLAAGVSVYYIPSIIRAMSSGDDKPASNAVSTGSVK
                     RATGLSDDVDPFNTREEPAKTEERKESSGKAETPPEKVQQNFSRALDVAYGGSSSASS
                     NSGGSSSTASNTRNEGDGSDKQAEVQPVNAGQPAQLSKITRVPYDPNLFIPENTAIKC
                     SLDRRFISDLAGKLVCTINEDVYSANRNVKLIEKGTAAYLMYKTGTFNHGQGAVFIAA
                     TKLRTRKEPFIDIPLIDTQAAGALGEAGASGWIDTHFADRFMGAMMVGMIPDVAQAAS
                     GAAKSNKDNQTDYTANSRQAFADIAREAFANSVNIPPTLYKNQGEIITLIVGQDLDFS
                     SIYKLKMVGTRR"
     gene            complement(14091..15020)
                     /locus_tag="L2X49_002698"
     CDS             complement(14091..15020)
                     /locus_tag="L2X49_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HBN4797767.1"
                     /translation="MKMNKGALIVALLLVSHVCHAAVTPAASRFDPRNQIVSYNPQNT
                     TVINSAVGYTTTLVFDEDETVISARTGFPQGWEVNKEDNLVYLEVKPVKQTVQKNSTD
                     DNGNQSSEVVSVALDPENDLERWRTNLFVRTTKRNYSMELNARTFRQPDKIAFVVNYQ
                     YPQERRKEQAEIEKKRIEALNKRQEEQAINRSLENAKSPKNWDYWKRVADGSQSISPD
                     YAYDDGRYTWFGFSPLKKIPSVFVMSGEQETLTNPVVKNSGSFTVVGVPVDQRFVLRM
                     GNQVVGVENRGFGKVRLPAGDTVSPNVEKEVIQ"
     gene            complement(15026..15754)
                     /locus_tag="L2X49_002699"
     CDS             complement(15026..15754)
                     /locus_tag="L2X49_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBN4797768.1"
                     /translation="MGFKLSGLKLPGFNKKKEVTDSSTTFEEKNVLMQEKMNRIYKFG
                     GIAGIAVGVLSLLALNGALPLKSTVVEAYLINGVTGVAERLTSVKKENLSENEALAKY
                     FITQYVKRREGYNYFSLQHDYDYVLLYSAENVATDYNALINGNQSPKVIYNKAEKTAS
                     VQDNPSVIISPSSRRDDKDMGAYIRFKLTIRNVATGQDDYEYWNVRLTYRIEPQVEMA
                     SGDRNNNPLKFVVTSYVRDKEVKG"
     gene            complement(15949..17004)
                     /locus_tag="L2X49_002700"
     CDS             complement(15949..17004)
                     /locus_tag="L2X49_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBN4797769.1"
                     /translation="MATSRYFQAAHDVIVKTLDQSLTGQLDNFSSIASQLGKYGISIY
                     ILWYAYTTIVGKQKTAVQDFLWNLCRFWLILVFVKNMGGWLDSATQAIDGLKETFAGG
                     DPWKWVDELWEKVQQVAAYLMSKDTSKYVKTDGAIASLLTYAGGIIALLLTSIVYLAA
                     EVTIKILTITAPLFIICLSFGFLRQMFNSWLQLIFSSCFIFLFCGLAIKAGMTFLNGI
                     LTISIANADELNLISTGAQAGVAGAFMAWIIWQAKTYASQLAGVGVEGAMQGAAAMGI
                     GAGVFGASRMGRNLLGMGKNAGIGAWKGVRRQDGGFNQSPGVSGKLGNLTGQGVNIGA
                     KRLRQAAIDAAKKKYGG"
     gene            complement(17016..17273)
                     /locus_tag="L2X49_002701"
     CDS             complement(17016..17273)
                     /locus_tag="L2X49_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HBN4797770.1"
                     /translation="MKKYGLVAALGLTFFLTGCEEVKSVDWWQSHVDDATKKVAECKK
                     SGSDSENCKNAKEGLFRYKQLHPKHVDYKDAFKNVLGKDGS"
     gene            complement(17283..18053)
                     /locus_tag="L2X49_002702"
     CDS             complement(17283..18053)
                     /locus_tag="L2X49_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HBN4797771.1"
                     /translation="MKKHIVAALIASVTVISGAQAGVPVAIDANPEWAVEAQRWTERL
                     KQWQDTVNHYQKQINAYKQELLTKTGIRDVQGLVQSAQSVSKELENIYDQGNSFIDDY
                     IKNPEATLSEQARSLLADYKVTNTCKGLGYTGDLVRGCEASFLSQLAGIEYGNKLESK
                     LREDNQEMADLIDQVKNAQDTKATQDATNAVSLASLKFNKLKFQYEMYRDKQRDLAEY
                     KEKMLQAAFQEKQLGAVNKETPVVDYKAAFEQQSYEMN"
     gene            complement(18063..20815)
                     /locus_tag="L2X49_002703"
                     /pseudo
     CDS             complement(18063..20815)
                     /locus_tag="L2X49_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247738.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
     gene            complement(20840..21130)
                     /locus_tag="L2X49_002704"
     CDS             complement(20840..21130)
                     /locus_tag="L2X49_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HBN4797772.1"
                     /translation="MLKLNKRYLTLSVFMAALMLCVAEPAFADDVSTKTTGFLQKIID
                     FLTDIRKPAITIIALVIGYIAIFSRQHAAWITPLIIGIIIFIVAPYLPDWLA"
     gene            complement(21114..21758)
                     /locus_tag="L2X49_002705"
     CDS             complement(21114..21758)
                     /locus_tag="L2X49_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBN4797773.1"
                     /translation="MLSTTAFAALALQCAASVHPDTAHEVARVESGFNPYAIAEIIPK
                     VERKPGDKGVVSYFPKTKEAALQIVNQIESRNHRYSVGLMQITSTNFANFNTTAEKMF
                     DPCENLKVSEQILVDCYKRGGDILRGLSCYYSGNPETGTKPESDFNNTSYIQRIGFNP
                     PDNKKNWVVPSVKDAIRKENVTQSIKPKEVTVYPQYAMRGTVLDEKETNDVKTQ"
     gene            complement(21991..22500)
                     /locus_tag="L2X49_002706"
     CDS             complement(21991..22500)
                     /locus_tag="L2X49_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HBN4797774.1"
                     /translation="MEWYVLQFTTTRFAAVFAHLERLNFSYFCPMETERYRRPDKIIS
                     YRERRLPLFPGYLFIQADFEEVHSTTITAIPYVQRFISFGGEPLPVPEDVMAELLYRQ
                     SHTTAQANLLRKSIPHDFAEILLMDNPQQRSMAFIHYITERSLTHKMKRKKNDCYPKK
                     NRNETQAPT"
BASE COUNT         5897 a         5537 c         4751 g         6418 t
ORIGIN      
        1 gttaccgatg caagaacagg ggagtttcat gaaatgcctg tttaggagtg attaaaggta
       61 acttgtgcgc agaaggtcag gtgtgtggtg gttagtacag actagtgaat gttgtaacta
      121 aactatattt ttattaaagg ccggaggtgg tgttcctccg gccttaatta cgtttccgtg
      181 ttcattagtc gatgaactgg cggatcagtt ccagaatggc aatcagcgcg tttgcaagcg
      241 ccgctaccgc cctcagaacg atgattaaca ggttaatcat ccgttttctc cataaatgga
      301 gtaaagacac cgtggcttgc tcttacactg cctgtcacca cggcttctct tcgtcttgtc
      361 agttgtagag agtctcactc ctgccagagc acagataacc agcaccacct gtattacctg
      421 tcaggacttt gaacctgttc atgccgcatt tagcggaatg cagggagaga ctcaccacag
      481 agctaaccct ctgattatac gtttatctaa cgttcaggca aaccgttaac gcacagtcac
      541 tgcccgtcat attgcataat caggcttcac ggattatcat actattgtca gttgtagaga
      601 gtttcccttc cgggctgagt taccagctcg caccggaacc gtaaaaaagc cgacctcacc
      661 agtcggtttt tttacgtctg cggtttcccg cttcgtttaa atctatttta aatgaataaa
      721 ttgttctgcg gcaaacccaa caacataagg acgttagggt tgatggttgc cgaaatcgcg
      781 gtgcgttacg tgaattctga ggttttgcga gggggattgg gcgcgagaat ttacgagaga
      841 gcgccggaac aatgccggga tcacaaacca tcacccgtga aagaaatctt ccggcattgc
      901 ccggtcaggt agtatgacat tcattgtcaa cggccatcaa tttataagct gctttgcact
      961 tcataaaaga tcaccaccag ggctgctaaa acagtaccga tagccagcac gttaaaaagt
     1021 acgtccttca tctttacgaa cgacatactg atttgcgtcc tttccctttt gtcagccttc
     1081 tctatcatcc agcgttggta tgaatcactg ttactttttc tgtccatgtt ccccccaatt
     1141 catcaatgag caccaggcgg ccaccaggct tcttgccaac aaagtaacgg cctcgcggca
     1201 gacgttcgcc agtcttcagc cgttcgatcc agtcctgaaa tgcagggcgc ggtttatagc
     1261 tgtagcggcc aaaatcagct aaagactgcg tgaccatgac tgtttggcag ccatgctttc
     1321 tcatatcacg aaacgtattt tccagtgcag ccagtgtatt catttgcacg ttctctctgc
     1381 ttttcccatg aagatattaa cgacactcaa aactaacacc gttggcatga tcacggcagc
     1441 atagccgaca gcaacggcct gatatatggt caatgagtag tcgggctgtt tatacaatac
     1501 gactgaaaaa atgctcccca aacaagcaag cagagttaaa aacaagtaat cgacaaatct
     1561 gtttttatag ataacccatg caacgatgca gggatatgac accaggcaca ggataatcgc
     1621 atttgtggta tgcacgtttc gctccttgtt atttaagggt tgtcagccag atcaaacaga
     1681 ttgttcaggc tggaacggaa ttgttttacc gatgatacca gctcctgact ccgcgctttt
     1741 tcctgcgcca gttcacgccg tagctgcgcc atctcgtctc tgttacgctg aagctcaagg
     1801 atagccgtca taaaggtttt gctggctgat gcctgtccgg tcgcttcctg cgccttttta
     1861 atggcctgat cttcgtcgtc attcgttctg attgtgtaat gccccatgat acccctcttt
     1921 tgctgtcatt tttgcgctgc cggcgcgaac ccgggaagaa actttgctgg caaaaaacac
     1981 tggccaacag atgtgaccgg gaaaatttgc tggcaattaa ttttcgatga aagccagctc
     2041 gcaatcgccg cattcaatat tcagtccggc tttgccccac acggcagcgc cacacccgcg
     2101 acactgatat ttaactttat ttcgttttgg cttgtcctca aatgctgcaa ctgacaaatc
     2161 attgtcattg ctcccggcca tatcatctga atggctcccg ttctgcggtg acactgacgg
     2221 cagcagagcc atagaaagca ccgcttctat atcaatcccg gcctcagcat tttgatgcgc
     2281 cagagcaagc gtttcgcgcc attgctctat tacggcggtc atatcttttt gttgttcgac
     2341 ctggacaggg aagcgatcaa accagctcaa agcaaattgc ccctggaaca gttccagtgt
     2401 gacgcgctgg aagcggccac cagggatcgg atagtcatta atcttctggc cgactttcgc
     2461 accaccaggt ctcccggtgg aggagggcat aagtccgata ctttccattt ttgccgccca
     2521 ctgcgcattg tggtacgtct tacgcgacgg gttgccgtag tggtactgcc acatatggca
     2581 ctgctcatga accagcgttt gcatgacttc aataagcggg tacaccggga agtattcagg
     2641 attgagggcg atttcatcaa tcatcctgcc gctgccgtca gcagcaacaa agcgcctgta
     2701 actgaaatag cccattgtgt tcttgccgcg ctggaacgta atcagacagt cagggagttc
     2761 gccgtcaaaa aggcgctggt tatagaaatc aaacgcctgt tgtagctcgc tgtaagcgcg
     2821 ttccgtggct cttgtcatgc tgaagttcgc ttagcggcgt tccgatattc ctgtaactct
     2881 gcaacaatag cagggaacac atcagcgcaa cgctgtgcca gcttaaccgc ctttgaacaa
     2941 acgcccatac catgtgctct tttgagcgcc tccaacaaat ctaccagttc ctcgtcagtg
     3001 attttttcag tcatctatta agcttctcca ttatctgtat cgtacgatac agataatata
     3061 aaccacacct ttgatagcgg caatcttttt tgctggcaat ttcacgccca gggcgggcat
     3121 cgagcgggta atattgctgg caaaaatcaa cggccaaacc ggctgcagga aactggtttg
     3181 ctggcaagcg tgatttactg ctgggcttga caccttgcca ttgtatgacg cttgttttag
     3241 tgtcaaatga acaaccagcg cgagaagaat ataggaagca aaagcaatgg atatcaaacc
     3301 taactcagtg atccacctgg agccaggcga aacattaccc ggctatacca atctgaaacc
     3361 cgtacctgat aagtatttcg atgagttaaa ggcactcata aataaactca atactcaatc
     3421 cggtaatatt tacctcaagt taaaacagat gtacgccttt ttggatcgtt ttaacaaaga
     3481 atttgtgagc acgtttacct cctgtcaaaa ggggtgctct tcgtgctgta aaatggatgt
     3541 tcatttaacc gcgcttgagg caacacatat tgcacaagca agtaaactta ctgccagaga
     3601 caacccgctt acgaccgggc atgagtcaaa atgtccgttt ttgtctgaga agggaacatg
     3661 ctcaatctac aattaccgcc cgctgttatg caggacatat catgtactca ctccgccgga
     3721 gatgtgtaat gacctggatg cacaagtcat gcagtatggt tctcaaagcg cgaacatggg
     3781 aaatcacatc tacaaaacaa tagcagagtg gatctatttt cagacgtatc actgcactgg
     3841 taagttagaa accaaagata tcagagacta cttcccttat ccccgcgaag atatccaaag
     3901 atttcttcac cataaccccc cgcgcccttt ttgctaaaaa aaagcctctg acgcagtgtc
     3961 agaggcttgt tagattactg ttcgtcacca gcagtgctgg aagcatcttt tttgatgaga
     4021 aactcatcaa gagaacggcc agcttttaac gcttcgtcga ttggttttgg tgtccggcca
     4081 cgaccagacc agtatttcgt ttcgccgttt tcatcaaact gatatttagg cggcgctttt
     4141 ggcaacgtct ttttgcgtgt tttttgcgca tcttcggaca ggccaagcag ttcttccggt
     4201 gaaaaacctt ctccggcaat caactggagc agttcctggc gctttctttc gcgctctgcg
     4261 cgttctgctg cctcacgctc tgcttcttca cgacgttctt cgatcacggc gttaagcttt
     4321 tcctgcattt ccagcagttg ttcaaagctg gtttctcgcg caaaaacacg aacagagcga
     4381 atattcagga gtagcttttt tgttgcgtca aacgtttcat tttcactcat cgagttaatc
     4441 cttatttttt actgaactga aacccaagtt tacccgtagt tttatcctgc aaaacaattg
     4501 cagcgtcaaa ctttttcccg gttttattac tggtaaagcc tttaatctct ccggttttac
     4561 cttttttgat taatgtctca acctgatttt gtgtcagttt ttttcctgac acttcagacc
     4621 agattttaaa ctcgcacccg gaacagaaaa agcctttagg ccggataata atttctttac
     4681 tgcatgaagg gcagggggct tcgagccggg acatggaacc ttctgatctg gattttgctt
     4741 ccatatccgg tttgccgtct ttgtccggga atgttgtctt gcattcaggg tagcaggagc
     4801 atccccagaa aaagccattt tgccccttac gcttacgcag gaaaccgttg ttacaaaccg
     4861 ggcagggcgt ggcatttgaa gaaatcgaaa ttccgttcct gtccagctcg tcgattaacc
     4921 cctggacgta ctcatcattt tccttaataa actcctcaac tgtcagctct ccggctttga
     4981 tctgagactg cttttcagcc cacagagcgg agatatccgg tttagtgatt tcaggaggca
     5041 gtgcagcgca aaactcctgg ccttgttttg tggttttcca taccagctcc gaatacccct
     5101 tttctttttc gatggagata agcccggtat ttgccgccag tttctcaaga atacccgcgc
     5161 gggtggcttc cgttccgata ctgccacgat cactgctgcc ctcgtctttc gcttccagcg
     5221 cctttctgag agccggatca tcaatgaatt ttgccgcact cgtcatggcg gccagtaacg
     5281 ttgactctgt gaaatagcga ggtggtgtag tctttttctt gtccacgtct gccgattcac
     5341 acaggccgct gtcattaaat ttcagcaatg aaaggtcaaa accgtccgtt ccggcatctt
     5401 cggtttcgtc ctcgtcatca ctatccttac ccagcgcttc ccagcccttt tgtaccagaa
     5461 cgctctgtgt ggccgtaaag gtatccccct taatatcaaa gtggattttc gtcttgttgc
     5521 ggatcgcatc cgggtagaac agcccgataa agtagacgga caccaggtta taaacgttcc
     5581 gctctttttc attaagctga ataccggctc cgctttttgt cgtcgggatg atggcgtggt
     5641 gcgcctcaat cttactggca ttaaaggctt tgtgtttctg gcctttatcc atatctacga
     5701 tcgcagacgc cagttcgggt acggtggccg ctatagcagt ggcaatatct ccggcctggt
     5761 aataatgctc atctgaaaga tagcgattgt cagaacgtgg ataggtcaga agcttgtgag
     5821 tttcatacag cccctgcatc acatcgagcg tatcttttgc agaataacca aatcgctttg
     5881 cgcagagctg ctgaagcgtg gaaaggttaa gcggaagcgg aggcttgcta ttttccggct
     5941 ttgtcgtcgc gatagtaaca atagcatcct tacccgtaac acgtccggca atatgcgcgg
     6001 cctgagcttc ggagatcagg cgttttttct cgtctatctg atcgtcgtct gttgtctgat
     6061 atttcgcctt aatctgatgc ccgttcatcg acagactggc gaatacatcg taataaaagc
     6121 tttctgtgtg attctggttc gccagcgtgc gcatgttcac cagaccaaga acggcacttt
     6181 gtacccggcc aacgttcaga acgccgtcat agcccttctc acggcctttt aatgtacatc
     6241 cccgcgtcat gttatagcca aaaccctgat cgcacaggga acgcgccagg gcgcttaacg
     6301 tcataccgcg aaatttatcg ttaggttgta aattcgacag tgatttttgc actggcgcaa
     6361 ggttgaggtc ggcaacaagc agtcgctgga cgggtttctt gtttccggca taacccagaa
     6421 tctcatcaac cagcaaacat ccttcatcat cggggtcgcc tgaatgaacg atgctgtcgg
     6481 ctttatcgat aagagacaaa ataatgcggg tctgtgcttc tgattcaggc ttgattttat
     6541 attttggcgg ataaaccgct ttaatgggta aatcactata acgccagttg gaatatttgt
     6601 cgtcataatc ctgtgggtcg taaagctcca gcatatgacc ataacaccag gtaacgacat
     6661 cagaaccgtg ttcaaaatag ccgtttttct tttctgtatt gggatttccg cccagtcctt
     6721 caaaaatagc ctttgctaaa gacggttttt ctgcaataaa aagtctcatt aatcaatcct
     6781 caaggtaact tgaatagtca ggctgcgcac cattcttttt aaagatttct tccctttcct
     6841 ctatgtcttc ttctgcctcc tgcacactaa ggtcaagggt tcctttcaaa aaggcacgcg
     6901 cattcttgat tacatctact gttaattcgt tttttgttcc gcgaatcatg ccaatagcaa
     6961 tatttattat ctcctttatt tcagcaggag tcgttctgtc cagcaccgcg ccaagttctt
     7021 taaggtcaat atttatatcg tctggtaagt acattttggc acaagcggca gcggctttag
     7081 tccgaaaagc acctacagat atgattctgt caattctgcc agggcgtttt ctgatgcgcg
     7141 gatcaatccg gttaggatag ttcgttgaaa aaataacata tgtcccggca agatttcgcg
     7201 gtgtatcagc cccatcaaga aaacttaacg ttccggcatc agcccgataa agttcatcaa
     7261 cctcttcagc tattatgata acgggtattt tcttttgagc tgcggcatag caaacatctt
     7321 taaagtaatc atcagcataa acaaatacat atttatcctg atattttgcc cccaaagctt
     7381 ttgcgatagt cgttttccct gtgcccggag gaccagtaaa aagcactttt cgcattcctg
     7441 actgaccata acgggtgtag aaatcaatat ccgaaaagaa ctgattaaag tcattctcca
     7501 gctcattaaa ctttggatga acagaaaaac gtaaagcgtt ttctatttct tccttccttg
     7561 ccttaccgtg ataataaacg ccgtcgtaac acccgatttt ccagatacct ttacggggta
     7621 aagagcggtt taatttagcg cgtccagctt cgcaaagatt atgaagcatc gacatacttt
     7681 tggttgtgcc aactgcgtac tccattctga ctttagagtt aagaatgaag cgaataatat
     7741 gaaccacttc attttgacca agatgaatcc ggtatgctcc cgaaccatct ttaaacataa
     7801 gctttttaat cctcccggct ttagccataa cagggctact tcccgtatga acgacggttg
     7861 aagcaacggg ttcgatatca agattcgaat aaattttacg attctttctg tttataaaaa
     7921 tctctgccac tttaggatag taatcacaat cctttacgac agtaccaaaa ttatcttcac
     7981 cgtaatattc tttatggagc cgtaaagcct gctctaccag agttaattta cccatatgcg
     8041 caccatgtat attaaaaacc gtgacgttgc ctgaagattt tcttcaggca gaggaggggt
     8101 tggacaagcg aagcgcgtca gttttgttta tgacgccact cccacggagg aacgggatta
     8161 ctgtcagccc acaggccgcg cttctgtgtc tgagcttccc gttgcagagc cggtaatgag
     8221 ttatcggtgt tgtacctgtc ataaacccat gcagcccctt ttagcacttg ctggcggtta
     8281 gcctcagtgc cgttcgctgt caccactcgt cccaggactc gcccgtagcg atctgtttgc
     8341 gtataagaga cggtaactga ttgccctgcc agcagggctt taagttgatt ggttgaccag
     8401 cgaccaaagg gttgtttttt ttccggtgca tcgatattta tcaatcggac tcgaatcgga
     8461 acttcataca caacaatttt cgcgggtagt gttttaactt cgatggtatc tccatcgaga
     8521 acgcgaataa ctttcccctg aatctgagca gcacacaatg tagctggcaa cagggcaaca
     8581 gcaaaggaaa gaacattaaa aaaatgtctc attgctttct tccctcccat attgctgaga
     8641 gtcttttaaa aatggataaa acgacgacta aagccactgc gataacagcc aacgaaggaa
     8701 gtacatagct gtataactca accatgaaat taataaactc aatgagcgat tgcggcttta
     8761 aggaagcatc ccacaaaacg ataacaaaac gataaagccc atagacaatt gccggaaacc
     8821 agaccataaa caacaaaatt tcagtaaaaa cagaagcgac cgatttgaca ctatcgataa
     8881 tttcaattcc ggcaattctc acacttcttg catcaggtac gttaataatt ttcatcctcc
     8941 cacccctgta aaaaatgttc gcaagttcca gtctcaaacc tgaaacttgc gtattcacat
     9001 cagaattaaa tcaaacagaa ttaaaagttt ttcaatcggc caaagcttga aataatttta
     9061 cttactgtac ctgcatcagc cgcaggacat tcatttagtt ttttctttct ggcatctgcc
     9121 gtgtgatcgg gtaaaaaacc atgtttattc tttttcctga ttgacataaa acttttatac
     9181 atcgggtcac attctgccgg actacttccg tcaagttttc cggcaaggca taacaccatt
     9241 gcacatggat cgttcgggtc ggccttttcc tcagcatgag cagttccggc agcgaacgca
     9301 caaagcagca ctgccgtggt tacggtcttt ttcatataca cctcatggat aacgggcaac
     9361 taacgccctg cggttgttca accccggctc aataaaatta caagaatttt attaatccgg
     9421 cgttatcgtg ataaataaac actggtagtc cggcaagaaa aacagttcat tttataacga
     9481 tactgtatga caccgtttgc accgttcgcc ctcaaccagt ttttagctgt gaaaaagtct
     9541 gttccgttac tggtttcaac aatcactgaa tcagaatgtg caacagaata aaaaacagca
     9601 tcatcgtaaa ggcggttttc aggcctgaaa ttatggataa ccttaaccca cgatgaatta
     9661 accttgtctg atttaataac tgagtatgtc ggttgccagt ccattaattg tgtattcacc
     9721 gtttcaggat tctgttccca gtcaacctgt ggtggttcag gcggagagga acaggcagag
     9781 agaaaaagga cactgaccat taacattttt ttcatagtaa aacctctgac tccagctcac
     9841 cgacggagag cattttttct ttagaaggat atttaagccc tttaacaccc aatacttttt
     9901 tagtcttatt cagagaggcg gttaactcgg taagtttggg gcttaccatc atcagcctgt
     9961 ccataaaata cgggtcgagg aagtagaggg ctttctcagc tttaaccggg ttttcacctt
    10021 taagaatgat aaattcttct ttaaaatcca gcgttcccag ctcctgcggg agcacaaggg
    10081 cgcgctgcgc ctcactttct gaactgcttt tcgacgttga gcgtccgctt gtcttgcttg
    10141 aacctgtcga tttggtggtg atatagccca gcttttccga aatcttttta gcgtcatctt
    10201 cttcactgac agcgtagata atccggcagg gatgcgcact catcagcgtt ttggcacctt
    10261 caacaccata gatttcatta agctgactga tattctggta aatcgtcaga agtttgagtt
    10321 taaacccggc aatataaccc gaaccttttt taatgattgg catgtaacca atagacggaa
    10381 actcatccag gaacatcaga cagtcatgtt tcagtgtcgg gtcaaagtca gggttttctc
    10441 gtaacgtgac ttccacaacg aagttaaaga acaggttgag gaagtcatag gccagggaca
    10501 tatcttcagc attaacgcca acataaacag tgatatcttc ccggcgcaac tggcgaaggt
    10561 cgaaatcatt gccgtctgta catttgcgaa cagtcggaag ataaaacaga ctcattttct
    10621 tacgaaacga accatcaata ctcgaacgtt gttcgtcttc ggtttcgtga tactccctga
    10681 ttttggtcag ggcatcgcgc agatgataca gcgcgttttc atcaagtcct tttgttccct
    10741 ccaggtcttc tcgcttactg agtatcagct cccggtcgat gttgctgtac aaatcgacta
    10801 cagaaccaat tgagaaaaca ggtttaagcc cgaactcacc aagccaggag gggtcgtagt
    10861 taataaagaa atgaagcaat tttgccagcc ccgtccagta ctgccccgcg aggttattaa
    10921 agtgtgcttc cgctccggtc agaccgtaag acgggaacag gatctcaatc agcttcagca
    10981 ggtctttagc cccgctctct tcttttaaat caatatagaa aagcgggtta aactgatgcg
    11041 ttttactgtt gaaagggtca agaagataga ccttattacc cagcagtttt tcacgtacct
    11101 tactggtgat tttccacagt tcctgttttg gatctaatgc aatcagggaa tgtttcctga
    11161 ccagcaggtt tggaatacct attgcagccc ctttacctgc gcgggttcct gcgccaagtg
    11221 atacaaaatc tggtgcggta taccacaggt atttaccttt atatgcacct acaagaatat
    11281 cagtatcgtt ttctttctcc catttcagaa gtttcgattt tcttaaatca ttatcactgg
    11341 caaacttcgc gtcaccataa agagcaacat ccgttttatt cagttgccag ataataaaaa
    11401 caggcacaat gagagacgaa accatacctg ataaaagaga ggggattgcg gttaacctga
    11461 tatctgtccg tatattactc tctgttatta tccgccacag cagcatggaa tcaaaattct
    11521 tccatatata aagcggcgtt tttccgttca ggaagtaaac agcaactgag ccagtataat
    11581 acgtcacaag acacataacc agaccgataa aagcccactg gcctttatcg ggtagtttca
    11641 gagacataat cactccttca gcttttcaaa gtattcctga aaatgaatat cacgatagta
    11701 aatacccgat gcaaatcggt tatcttcctc atcgatgtac ttaatactca taatcacatc
    11761 gacgttgctt aacaccctcc gcaatataat gttataaggc aaattctgac attcagggtt
    11821 catatagcac cgttgaacca gtccaagcac agcatcttca ggggtgctct catggacggt
    11881 tgtaaggttc cctgcatggc cggaacttga gcctttgaga aaatcccagg cttccgcgcc
    11941 gcgaacctca gtcatgagga ttctgtccgg gttcatacga aaaccagaac gtaaaagaga
    12001 agcggaactg acagtgctac tttccccttc tgacgggtaa tacaatttca catgattttt
    12061 gtggaacctg aactttgctt catctgtatc ttcaattgaa atacatcgta aatgatgggg
    12121 tatgtactga agacaggcat ttgcaaaggt ggttttaccc gcccccgttc cggcacaaaa
    12181 taccatcgtt ttacccttaa ccaggcattc agggacaaat tgttcaaaac aattatcttt
    12241 aaacattgaa gataattcat cttctttatc cctgaatttc cccccttgat ttagccttga
    12301 gtaaaaccct tgtttaacaa aggtatcatg gtcgataaac acactggacg gcttacggat
    12361 tgtaatagac accgtttccc gttccgttgc cgggggaata actatttgta cacgctcccc
    12421 tccggggagc gtaacagaac gtaagggata ttctggcgta accgacccac catcatgata
    12481 atcagaaatt gcccctgcaa atgacataca atctttaaaa tccattttta attcatgctg
    12541 ctgccatttc ccactcactt taacaaacag ttcatcaggc cgatttaccg cgatttcagt
    12601 taaaccatcg acttcattaa gccagtggtg gaagtattca tctattattt cataaacaat
    12661 atgtctgttc tctgcatcct tcataattca ccttttcctt taagatcttc aataaaacgc
    12721 atgacttctt cgtaattaaa tcgatgcgtt ttgatttgtt tcagtggttt ggggaaagga
    12781 acgccattta ttggtatgcc cttacgaata tgatggtata tttcgctttc ccttattcct
    12841 aacagcattg aaaactcttt gatatccagc gttatcaaat tacctccggg tacccaccat
    12901 ttttaatttg tagatgctgg agaaatccaa atcctgacca acgatcagag taatgatttc
    12961 cccctggttt ttataaagcg ttggagggat atttacgctg tttgcaaacg cttcgcgtgc
    13021 tatatcagca aaagcctggc gactgttagc tgtatagtcg gtctgattgt ctttattact
    13081 tttagccgct ccacttgcgg cctgtgccac atcggggatc atgccaacca tcatcgcgcc
    13141 cataaagcga tctgcaaaat gtgtgtcaat ccagcctgaa gccccggcct cacccagcgc
    13201 tcccgccgcc tgtgtatcaa tcagcggaat atcaatgaaa ggttccttac gtgttctaag
    13261 cttcgtagca gcgataaaca cggctccctg accatgattg aacgtgcccg ttttatacat
    13321 cagatatgcg gctgttccct tttcaattag ctttacattc ctgtttgcac tgtatacgtc
    13381 ctcattaatc gtacagacca gcttgccagc cagatcagaa ataaatcgtc tgtccagcga
    13441 gcacttaatc gcggtatttt caggaatgaa aaggttaggg tcataaggaa cgcgcgtaat
    13501 tttagaaagc tgcgcgggtt gccctgcatt aacaggctga acctctgcct gtttgtcact
    13561 gccgtccccc tcgttcctgg tattgctggc cgttgatgag ctaccaccgg aatttgagga
    13621 agcagagctg ctccccccat aggccacatc cagcgcacga ctgaagtttt gttgtacttt
    13681 ttccggtggc gtttccgctt tgccgctgct ttccttccgt tcttccgtct tagccggttc
    13741 ttccctggtg ttgaatggat caacatcatc gctgagtcct gtcgcgcgtt ttactgaacc
    13801 agtagatacc gcattactgg caggcttatc atcaccgctg gacattgcac gaataatcga
    13861 cgggatatag tagacagaca ccccggcggc cagcagaagg aaagcggcca caataatcac
    13921 cagcccctta cgcgattttt taagcttctc gatacccagg cgttttactg atttaccgtc
    13981 atcttcatct cccttatccg ttttatcgtt ttgctcaatt tctttctgat gccgttcccg
    14041 tagtgcctgt tcaatttcta cagcggtcgg gattttattt tcctgttcgc tcactggatc
    14101 acctctttct caacgttcgg ggaaacggta tctcctgccg gaagacggac tttgccaaag
    14161 ccccggtttt caaccccaac aacctgattt cccatacgca aaacaaagcg ctggtctaca
    14221 ggaacaccaa caacggtgaa actaccgctg tttttgacta ccgggttagt cagcgtttcc
    14281 tgttcgccgc tcataacaaa gacgctgggg attttcttca gggggctgaa gccaaaccag
    14341 gtatagcggc catcgtcata cgcataatcc gggctgattg actggctgcc gtcagctacc
    14401 cgcttccagt aatcccagtt cttgggcgac ttcgcatttt caagagaacg gttgattgcc
    14461 tgctcctcct gccgcttgtt aagagcctca atccgtttct tctcgatttc ggcttgctcc
    14521 ttccggcgtt cctgcgggta ctggtaatta acaacaaacg cgatcttatc cggctgccgg
    14581 aacgttcggg cgttcagctc catgctgtaa ttacgttttg ttgtgcgaac aaacaggttt
    14641 gttcgccagc gttccaggtc gttttcagga tcaagggcta cactgaccac ctctgaagac
    14701 tggttgccat tgtcatcggt actgttcttc tgtacggtct gtttgacggg cttcacttcg
    14761 agatacacca ggttatcttc tttgttcact tcccagccct ggggaaagcc ggttctggca
    14821 ctgatgaccg tttcatcctc gtcaaatacc agtgtggttg tatagccaac cgcactgttt
    14881 atgactgtcg tgttttgcgg gttataactg acaatctgat tacggggatc aaaacggctg
    14941 gctgctggcg taacggctgc atggcagacg tgcgacacca acagaagcgc cacaattagc
    15001 gctcctttat tcattttcat agttttcacc ctttcacttc tttatcgcgt acatagctcg
    15061 ttacaacgaa tttaagcggg ttattgtttc ggtcacccga tgccatttcg acctgtggtt
    15121 caatacggta ggtcaaccgg acgttccagt attcataatc atcctggcct gtcgcgacat
    15181 ttcgaatggt taatttaaac cggatatatg ctcccatatc cttatcatcg cgccgggatg
    15241 acggactgat aatcacggac ggattatcct gaacagatgc ggttttttcc gctttattat
    15301 aaataacctt tggtgactgg ttgccgttga ttaaagcgtt gtaatccgtc gccacattct
    15361 cagcgctgta taacagaaca taatcataat catgctggag actgaaatag ttataccctt
    15421 cacgacgctt cacatactga gtaatgaagt atttcgccag cgcttcattt tcagagaggt
    15481 tttctttttt aacagaagtc agtctttcag caacgccagt gacaccatta ataagataag
    15541 cttcgacaac cgtactcttt aacggtagcg ctccattcag tgccagcaga gacaacacac
    15601 ctacggcaat tcctgcaata ccaccgaatt tataaatgcg gttcattttc tcctgcatca
    15661 atacattttt ctcttcaaaa gtagttgaag agtctgttac ttcttttttc ttgttaaaac
    15721 cgggaagttt taatcctgag agtttaaacc ccattgcgca ttacctctgc tgaattaaca
    15781 ggttcgcttt ttccggatac cggaggttgt ttatgtgacg cctggcatcc gacaaggcaa
    15841 agcgtcacga tagccgtgat aagtagtttc ataatgtgag aacctaaata gaggagtgaa
    15901 tatttactgc ggaatactca gagttgacag atttaaataa taaatcggtt aacctccata
    15961 tttctttttc gccgcatcta tcgcagcctg gcgaagcctc ttagcgccaa tattaacgcc
    16021 ctgcccggtc agattcccca acttaccgga tacaccagga gactggttga accctccatc
    16081 ctgacggcga acgcctttcc atgcgccaat ccctgcattt ttacccatac caagcagatt
    16141 acgccccata cgggaagcgc cgaacacgcc agcaccaatc cccattgcgg ctgccccttg
    16201 catcgcgcct tcaacaccta cccctgcgag ctgggaagca taggttttgg cttgccagat
    16261 aatccaggcc ataaatgcac ctgcaacccc ggcctgagca cctgttgata tcagatttaa
    16321 ttcatctgcg ttagctatcg agattgtcag aatgccatta aggaacgtca ttcccgcttt
    16381 aattgccagt ccacaaaaca agaatatgaa gcatgagcta aatattaact gaagccagct
    16441 attaaacatc tgccttaaaa aaccaaatga aagacaaata ataaacaacg gcgcggtaat
    16501 agtaagtatc ttaatagtaa cttcggcggc gagataaact attgaagtta gcaataatgc
    16561 aattatccct ccagcatatg ttaataaaga tgcgattgcc ccatctgttt tcacatactt
    16621 cgaagtatct ttggacataa gatatgcagc tacctgctga actttctccc aaagctcgtc
    16681 aacccatttc caaggatcac ctcctgcaaa tgtttccttc agcccatcaa tggcctgagt
    16741 tgcactatct aaccaccccc ccatattttt gacaaaaaca agaattaacc agaatcgaca
    16801 tagattccat aaaaaatcct gcactgcggt tttttgcttc ccaactattg tggtataagc
    16861 ataccacaat atataaatgg aaataccata cttacccaac tgagacgcta ttgaagaaaa
    16921 gttatctaac tgaccagtca aagactggtc aagagtttta acaataacgt catgagcggc
    16981 ctggaaatac ctagaagttg ccatagtggc tccttttaac ttccatcttt acccaataca
    17041 tttttgaatg catctttata gtcaacatgc ttaggatgta attgcttata cctaaacaaa
    17101 ccttccttag cgtttttaca attttcgcta tcactcccag actttttgca ttcagctact
    17161 tttttcgttg catcgtctac gtgagactgc caccagtcta cagatttaac ctcttcacac
    17221 ccggtaagaa agaatgtaag ccccaacgcc gcaactaatc catatttttt caaaatgccc
    17281 cctcagttca tttcataact ctgctgctca aatgctgcct tataatcaac aactggagtt
    17341 tctttattaa cagcccctaa ttgtttctcc tggaaagcag cctgtaacat tttctccttg
    17401 tattctgcaa gatcgcgctg cttatcgcga tacatttcat actgaaattt gagcttgtta
    17461 aatttcaggc ttgccagaga tacagcgttt gtcgcgtcct gcgtggcttt cgtgtcctgg
    17521 gcgttcttca cctggtcgat aaggtcggcc atttcctgat tatcttcacg cagtttactt
    17581 tccagcttat tgccgtactc gatccccgcc agttgggaca ggaacgaggc ttcacagccc
    17641 cgcaccaggt cgcccgtata tcccagcccc ttacaggtgt ttgtcacctt ataatcggcc
    17701 agtagtgatc tggcttgctc agacagggtg gcttccgggt tcttaatgta atcatcaatg
    17761 aaggagttcc cctggtcgta aatattctcc agctccttac tgacagattg cgcagactgc
    17821 accagtccct gaacatcacg aatgcccgtt ttggtcagca gctcctgctt ataggcatta
    17881 atctgtttct ggtagtggtt cacggtgtcc tgccactgtt tgaggcgttc tgtccagcgc
    17941 tgcgcttcga cagcccattc agggtttgca tcaatggcaa cgggaacacc agcctgagca
    18001 ccagaaataa ccgtaacaga ggcgataagt gccgcaacga tatgcttttt catagggatt
    18061 ccttaaatca ggtttgcacg ttccagatag gtatcaagcc actcttccgg cttcataccc
    18121 tctttccaga tttcatcgaa cagttcaagc tggggtttcg atgcgctgag aaccggtagg
    18181 taatacgccg cttttcccaa atccagtctt gcgaaagccg caaaatcatc actttccccc
    18241 ttcctctgtg ggtttttgac gaccaggaac tgacgagata acgggtcaat ggctttaatt
    18301 ttgtcaaaat aaagttctct cacttccaac tcgtctacgt actcactacg tttcgctttg
    18361 gggttcgcca gataaatatg tgttgcgcac tgctcacgaa cggcagccgc gataggtgat
    18421 ttaattagtt catccggcga ttgtgtcgct acgacaagca ccatatccag cttacgcgcg
    18481 gttttgagtt tgttataaac aaaatctctg aacgcttcgt tgttaatcca ttgccagaac
    18541 tcatccatgt agataaggag tcggcggcca tcagccagca tggtgacacg gtagatgaga
    18601 tagaatgaag cggcgctgga aagtactttg ttatccagaa actccgttcc gtctataccg
    18661 aaaacgtcca ggttgtccac atcaaacgta tcgtccgggt tatcaaacac ccagccaaat
    18721 tcccctccct gcacccaggc attcaggcgt gctttaagcc catgccgccg ggtttcagta
    18781 tcatcaggct ccagaatgag tggtcgcaac ttactgatcg gataagagcg gtttgacctc
    18841 tgcatcaggc gctcaacggc atcagaaatc aggcggttct ggtaatcatc aagcggctca
    18901 ctgttaagcg tacaaagtag cctcaccagg tctttcataa aggaaatatt tcgtttggtt
    18961 ggtggcaaag ccgcagggtt ccagccagtc ggctctcctt ctttcacccg aaagtaagca
    19021 cctcccattg aacgtatacc gggttccgct gcccgatctt tgtcgaaaaa tactgtagtt
    19081 aacttcttta atttcctctc aggtggaaaa gactccggcg ttccgaactg ctgggcggcg
    19141 aaagccttag tcatcatcac taccgtttta cccacgtttg acgtaccaag aatttcggta
    19201 tgccccagcg tggggttttt accgaagtaa ttttgatgct cggtagtcat gtgatagttc
    19261 aggttataaa tatcatttcc tgagccgccc agggtgataa ggttgttacc ccaggtattt
    19321 cctttctcct tgcccgtaaa gaaattatgg aagctttcca tttcggcaaa gttcagactg
    19381 ctgatagaac tcaggcgtgg ccgtaacgtg tagttaccag gtaactgagc aaaatacgct
    19441 gcgccaagac tcaaggttga ataggttacg atcagcccta aatcctccag ggtcgtcgtt
    19501 acaatattgg tgtccttcac cagacgctcc ggcgaatctg cgtaaacaat cagtgtctgg
    19561 tgacttttcc cgaaagaaat atagccgctg gagaccatat cgagaccaac tttcaaatca
    19621 gcgagcaagg atttagcggc atcatcggtc atttcgagtt tgtcgatctg atcatccagc
    19681 gccttgattg cagactgctt gtctatcgga gttaaagagg aagtctgtac atactcaacc
    19741 gggagataca tcagtgcatc aaaaattccg gcatccgttt cctgaagtag tccttgatct
    19801 cgatgcaacg aaagtaccgg gcatggtcag aggcagtgat ttgtccagca tcgttaccaa
    19861 agaacaggtc tttaccaccc aggtatgtat aaaacggact attggtgact ctgactttct
    19921 gccagcgccc ggatagcagt ttctggaaca gcgtaagctg ttctgaataa actatattat
    19981 tttcttcata taacccaaga cgacgggaat gaaaccggga gagataggtg ctcaatctgc
    20041 cacatatttc attcatatca ttaatgggtt cttcgaagat atttttatta ccctttttcc
    20101 ttgaaagaaa atgcgtcact ttatcttcag cgctgaacgg tttatagcat acggttaagt
    20161 ataatttgtt ttcaaaatac tcagccgccg aaagcgattc gtaataatcg ttcatcactc
    20221 tgttgacgaa tgggatcttg ctgtcaaata caggtcgcac ttcttttcta acccgaatac
    20281 gatgcgtata aaaggtaacg ggtttcccat caaaactacg tatcagcgta ttgagctggt
    20341 cagtaagcaa ggctagatcc gcttcatcta cacattcaaa ataagctccg tcaatctgcc
    20401 aggtcgccag tagatcatgg tttctggtaa caatgaggtt atcagtgatg tgtgatgaat
    20461 acgggattaa ctcttcaaga gaagactggt ctttaagctt cataaaattg tccactttcg
    20521 atatatcgac tgcgtcgtac gaactccccg cataatggac ggcattgaag cgcttgtttg
    20581 ccagaggatt acctttgatt tgcattcgca gataaagcag gtcaaaaaag cgttcatcta
    20641 tcttcgtaac tcgcttgatg gcataccagc cagggaggat gagggcatac atagcctcat
    20701 gaatccagac accaagcaaa acgcagataa cgcacattcc aagaaagggg tagagcggaa
    20761 cgcccagccc cctaatcaaa gcggggcgtg tcagcccttt aaaaacggta ctcattttta
    20821 cgcccccttt tattacatat tacgctaacc agtcaggcag atatggcgca acaataaaga
    20881 tgataatccc gataattaaa ggcgtaatcc acgctgcatg ctggcgtgag aagatagcga
    20941 tataaccaat taccagcgca atgatggtga ttgctggctt acggatatcc gtcagaaaat
    21001 cgataatctt ctgcaagaag ccagttgttt tagtggatac atcatcagca aaggcgggtt
    21061 ctgctacaca aagcatcaat gcagccataa atacagaaag agttaaataa cgtttattga
    21121 gttttaacat cgtttgtttc cttttcatct aaaacagtgc cacgcatggc atattgggga
    21181 tatacggtaa cttctttagg tttgatactc tgtgttacat tctcctttct gattgcatct
    21241 ttaactgaag gaaccaccca gttttttttg ttatcaggcg gattaaaccc tatgcgctgt
    21301 atataactgg tgttattaaa atctgattca ggtttagttc ctgtttctgg attgccggaa
    21361 taatagcaac tcaggccacg taaaatatcg ccaccacgtt tataacaatc caccaaaatt
    21421 tgctctgaaa ccttgagatt ttcgcagggg tcaaacattt tctcagcggt tgtattgaag
    21481 tttgcaaaat tcgtactcgt tatttgcata agaccgactg agtaacgatg atttcgtgat
    21541 tcaatttgat ttacgatctg gagtgcagcc tccttagttt tgggaaagta ggacactacg
    21601 cctttgtcac cgggcttacg ttcaaccttc ggtattatct cggcaatagc atatgggtta
    21661 aaaccagatt caacccttgc aacttcatgc gcagtatcgg gatgaacgct ggccgcgcat
    21721 tgcaaagcaa gcgccgcaaa agcagtagta gataacatga ttttctcccg gccagaacag
    21781 cgcggaaagt ttagactgaa tggttagtca tcgaagtcgt catcgtcttc atcgtcttcg
    21841 tttatcagct cctcaatagc ctggctggaa tattttttta tgaggtactg gacaacacca
    21901 gagcttgaaa tacgtcgccc cctgcgatag ccaatctcca gagcaatttt atccagttcc
    21961 tcacgagaag ctgagggcag gtagagtgta ttacgttggg gcttgcgtct cgttgcggtt
    22021 ttttttcggg tagcagtcat ttttcttccg tttcattttg tgggttaagc ttctctctgt
    22081 gatgtagtgg atgaaagcca tacttctttg ctgcggatta tccatgagta gaatttcagc
    22141 aaaatcgtgt ggaattgatt tccttaaaag atttgcctga gctgttgtat gtgattgtct
    22201 gtacaaaagc tccgccatca catcttctgg tacaggtaaa ggctcaccgc caaatgagat
    22261 aaagcgttgt acgtaaggga tagcagttat tgtagtggaa tgaacctctt caaaatctgc
    22321 ctggataaaa agataaccgg gaaaaagcgg caaacgtctt tctcgatatg aaattatctt
    22381 atccgggcga cgataccttt cagtctccat aggacagaag taagagaagt tcaggcgttc
    22441 gagatgagca aaaactgctg caaatctcgt agtggtgaat tgcaaaacat accattccat
    22501 agtttttcct ttcaggcgct ctctcgactc ggatgacccc taatcagaga aaaaaaacaa
    22561 aaggacaaat ttacatctca taaaaacttt atcagctaca tgt
//
LOCUS       DAFNBJ010000075        22452 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000075 DAFNBJ010000000
VERSION     DAFNBJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.075"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            4..546
                     /locus_tag="L2X49_002707"
     CDS             4..546
                     /locus_tag="L2X49_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4797775.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            629..1330
                     /locus_tag="L2X49_002708"
     CDS             629..1330
                     /locus_tag="L2X49_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBN4797776.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1355..3955
                     /locus_tag="L2X49_002709"
     CDS             1355..3955
                     /locus_tag="L2X49_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HBN4797777.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNHLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3946..5016
                     /locus_tag="L2X49_002710"
     CDS             3946..5016
                     /locus_tag="L2X49_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4797778.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5028..5570
                     /locus_tag="L2X49_002711"
     CDS             5028..5570
                     /locus_tag="L2X49_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4797779.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5578..6093
                     /locus_tag="L2X49_002712"
     CDS             5578..6093
                     /locus_tag="L2X49_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4797780.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGIPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6059..6796
                     /locus_tag="L2X49_002713"
     CDS             6059..6796
                     /locus_tag="L2X49_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBN4797781.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADKESIAILLTNTSQESWLINSKINRPTRWAGGEVSTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     VIAQCHVAWRAINDYGGLSAKKEQNLP"
     gene            6907..7917
                     /gene="pyrD"
                     /locus_tag="L2X49_002714"
     CDS             6907..7917
                     /gene="pyrD"
                     /locus_tag="L2X49_002714"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBN4797782.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8091..8633
                     /gene="zapC"
                     /locus_tag="L2X49_002715"
     CDS             8091..8633
                     /gene="zapC"
                     /locus_tag="L2X49_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBN4797783.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAL
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAMKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8630..9739)
                     /gene="ycbX"
                     /locus_tag="L2X49_002716"
     CDS             complement(8630..9739)
                     /gene="ycbX"
                     /locus_tag="L2X49_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBN4797784.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9983..12091
                     /gene="rlmKL"
                     /locus_tag="L2X49_002717"
     CDS             9983..12091
                     /gene="rlmKL"
                     /locus_tag="L2X49_002717"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBN4797785.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLVGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12103..14010
                     /locus_tag="L2X49_002718"
     CDS             12103..14010
                     /locus_tag="L2X49_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN4797786.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYLGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRYVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14140..15393
                     /gene="pqiA"
                     /locus_tag="L2X49_002719"
     CDS             14140..15393
                     /gene="pqiA"
                     /locus_tag="L2X49_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBN4797787.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15398..17038
                     /gene="pqiB"
                     /locus_tag="L2X49_002720"
     CDS             15398..17038
                     /gene="pqiB"
                     /locus_tag="L2X49_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBN4797788.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLYESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17035..17598
                     /gene="pqiC"
                     /locus_tag="L2X49_002721"
     CDS             17035..17598
                     /gene="pqiC"
                     /locus_tag="L2X49_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBN4797789.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17854..18021
                     /gene="rmf"
                     /locus_tag="L2X49_002722"
     CDS             17854..18021
                     /gene="rmf"
                     /locus_tag="L2X49_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBN4797790.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18091..18609)
                     /gene="fabA"
                     /locus_tag="L2X49_002723"
     CDS             complement(18091..18609)
                     /gene="fabA"
                     /locus_tag="L2X49_002723"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBN4797791.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18678..20438)
                     /locus_tag="L2X49_002724"
     CDS             complement(18678..20438)
                     /locus_tag="L2X49_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBN4797792.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20624..21076
                     /gene="matP"
                     /locus_tag="L2X49_002725"
     CDS             20624..21076
                     /gene="matP"
                     /locus_tag="L2X49_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBN4797793.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21152..22204)
                     /gene="ompA"
                     /locus_tag="L2X49_002726"
     CDS             complement(21152..22204)
                     /gene="ompA"
                     /locus_tag="L2X49_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBN4797794.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
BASE COUNT         5737 a         5272 c         5896 g         5547 t
ORIGIN      
        1 acgatgaaaa aaagtgtatt gacggcgttt ataactgtgg tatgtgcaac gtccagcgtt
       61 atggcagctg atgataatgc tatcacggat ggcaaagtaa catttaatgg taaagttatt
      121 gctccagctt gtaccctggt agctgcgacg aaagattcag tagtcacatt gccaaatgtc
      181 agcgccacca agttgcaaac taatggagca gtctctggcg ttaaaactga tgtaccaatt
      241 gccttagaag gctgtgatgt tactgtaact aaaaatgcga cgtttacctt tagtggtacg
      301 gctgacggcg tgcaacctac agcatttgcc aaccaggcta ccaccgatgc agcgacgaat
      361 gttgctctac aaatgtatct gccagatggt tctacatcgg taactcccgg taccgaaacg
      421 tcgaatattc agttagcgga ttctgctgag caaacagtga cctttaaagt tgattatatc
      481 gctacgggga aagcgacttc tggtaatgtg aatgcggtaa caaatttcca tattaactat
      541 tattaataga actcattaat tgttttatta attagtaccc ctctagtgtt ctggagggga
      601 tattcatatt ttttaagagt gactatttat gaaaacttgc ataacaaagg gaattgtgac
      661 cgtaagttta acggcaatat tactcagctg ctcatccgca tgggcggcag gtaaaggcgg
      721 gattggactt gcagcaacac gtcttgttta ttcagaaggt gaagagcaaa tttcactggg
      781 tgtacgtaac accagtccgg atgttcctta tcttattcag tcatgggtga tgaccccaga
      841 taataaaaaa tcagcagatt ttattattac accaccgcta tttgtgctga atccggctaa
      901 tgagaatctg ttacgcatta tgtacattgg tgcgccgttg gcgaaagaca gagaaaccct
      961 tttcttcact agcgtacggg cagtcccttc aacaacgaag cgggaagagg gaaataccct
     1021 gaagattgcc acacaaagcg tcatcaaact tttctggcga ccaaaaggtt tagcgtatcc
     1081 cttaggcgag gctccggcga aactgcgttg cacttcgtca gctgacatgg ttacggtcag
     1141 taacccaaca ccttatttca ttaccctgac agacctgaaa ataggtggaa aggtagttaa
     1201 aaatcaaatg atttccccct ttgataaata ccaattctct ctgccaaagg gggccaaaaa
     1261 tagcagcgta acgtatcgaa ccatcaatga ctacggggcg gaaacgccgc aactcaactg
     1321 taaatcgtaa gccgtcttca gttaagagag cgagatgtat agaactcatc gacaacacag
     1381 cctgttaagc tctggtggag tgccatcgtt tattggtggg ctggtggtgt ttgtgtcggc
     1441 agcgttcaat gcacaagctg aaacctggtt cgatcctgct tttttcaaag atgatccctc
     1501 aatggtggcc gatttgtctc gtttcgaaaa aggacaaaaa ataacgccag gggtttatcg
     1561 tgtcgatatt gttctgaatc agacaattgt agatacgcgc aacgtcaatt ttgttgagat
     1621 aacgccagag aaggggattg ccgcctgttt gacgactgaa agcctggatg caatgggtgt
     1681 taatactgat gcgtttccgg cttttaaaca actggacaaa caagcgtgtg tgccattggc
     1741 ggagattatt ccggatgcca gcgtaacgtt taatgtgaat aaactccgtc tggaaatttc
     1801 agtaccgcaa atcgccatca aaagtaatgc ccgtggttat gtcccccctg aacgttggga
     1861 tgaagggatc aacgcgctat tactgggata ttcgtttagc ggggctaaca gtattcatag
     1921 cagcgcagac agtgattctg gcgacagcta ttttctgaat ttaaacagtg gcgttaattt
     1981 aggcccatgg agattgcgca acaattcaac atggagtcgc agtagtggcc aaaccgcaga
     2041 atggaagaat ctcagcagct atttgcagcg ggcggttatt ccactaaaag gcgaactgac
     2101 cgtaggtgat gattatactg caggcgattt tttcgatagt gtcagctttc gtggtgtgca
     2161 gctggcgtca gatgacaaca tgctgccaga cagcctgaaa gggtttgcgc ctgtggtgcg
     2221 tggtatcgcc aaaagtaatg cccagataac gattaagcaa aacggctaca caatctacca
     2281 aacttatgta tcgcctggtg cttttgaaat tagtgatctc tattccacgt cgtcgagcgg
     2341 tgatttgtta gttgaaatca aagaagcgga cggcagcgtc aatagctaca gcgtaccgtt
     2401 ttccagcgtg ccattactcc agcgtcaggg gcgaatcaaa tacgcggtga cactggcgaa
     2461 atacagaacc aatagtaatg aacagcagga gagcaaattt gcccaggcca cgttgcagtg
     2521 gggcggaccg tggggaacga cctggtatgg tggtggacaa tatgctgaat attaccgtgc
     2581 cgccatgttt ggtctgggat ttaacctcgg cgatttcgga gcaatttcgt tcgatgcgac
     2641 ccaggcgaag agtacgctgg cagatcaaag cgaacataaa ggtcagtcat atcgttttct
     2701 gtatgccaaa acgctcaacc acttgggcac taactttcaa ttgatgggct atcgctattc
     2761 gacgtcgggt ttctacaccc tttccgacac catgtataaa catatggatg gctacgaatt
     2821 taatgacggt gatgatgaag atacgccgat gtggtcgcgt tattacaatt tgttttacac
     2881 caaacgtggc aaactgcagg tcaatatctc ccagcaatta ggcgagtacg gttcgtttta
     2941 tttaagtggt agccagcaaa cttactggca taccgatcaa caggatcggc tattacagtt
     3001 tggctacaac acgcaaatta aagatctctc gctgggggtt tcctggaact acagtaagtc
     3061 ccgtggtcaa cctgatgctg atcaggtgtt tgcactaaat ttttccctgc cgctcaatct
     3121 gttgctcccc agaagtaatg atagctatac caggaaaaaa aattacgcct ggatgacctc
     3181 taacaccagt atcgataacg aagggcacat tacacaaaac ctgggtttaa cggagacact
     3241 actcgatgac ggtaacctga gctacagcgt gcaacaggga tataacagcg aggggaaaac
     3301 ggctaatggt agcgccagca tggactacaa aggggcgttt gcagatgccc gagtgggcta
     3361 caactacagc gataacggca gtcaacaaca actgaactac gctctttcag gcagtttagt
     3421 tgcccattca cagggcatta ccctggggca atcgctgggg gaaactaacg tgctgattgc
     3481 agcaccaggc gcagagaata ctcgtgtggc gaacagcacc gggctgaaaa ctgactggcg
     3541 cggatatacc gttgtcccct atgccacttc ttatcgggaa aatcgaatcg cacttgatgc
     3601 ggcatcgtta aaacgtaacg tggatcttga aaatgcagta gtcaacgtgg ttcccaccaa
     3661 aggggcgttg gttctggcgg agttcaatgc ccatgcggga gcaagggtat taatgaaaac
     3721 atcaaagcag ggtataccgc tgcgttttgg cgcgatagcg acgctggacg gcatacagac
     3781 taatagcggc attattgatg atgatggctc gctctatatg tccggtttgc ccgctcaggg
     3841 agcgataact gtacgctggg gcgaagctcc cgatcaaatt tgtcatatca gttaccagct
     3901 taccgaacaa caaattaact ctgcgattac gcgaatggat gccatatgca gataatcttt
     3961 ggagaaaaat gcgtgttatt actacgacta ttttttgccg ccgtcttaat gctatggtgc
     4021 gctcaaactg ctgcttatag cgggcagtgt cataccactc aggggaatcc gtatattggc
     4081 gtcaattttg gcgttaaaac cctggaggaa gaagaaaata cggctggagt agttaaagac
     4141 aaattttatc agtggaacga atcgaatgat tattatgttt cctgtgattg cgataaagac
     4201 aatgtcagaa gtggccgatg ggcattcgcc gcggattcac cgttagtcta tttaggcgac
     4261 aactggtaca aaattaatga ctatcttgcc gccaaagttt tattgcaggt taaaggcagt
     4321 tctcctactg cggttccttt cgaaaacgtg ggcacagggg cagatacacg atggcatatt
     4381 tgcgatcccg gcggtcaacg tttaggtggc cagggggcta gcggtaatag cggtagcttt
     4441 tccctgaaaa tattgcagcc gttcgttggt tcggtcgtca ttcctcctat ggcgctggcg
     4501 cgattatttg aatgctacaa catacccgca ggtgattcct gcacgactac aggtacaccg
     4561 gttttagtgt attacctgtc tggtacgatc aattcacttg gctcatgttc cgtcaatgcc
     4621 ggagaaacaa tcgaggtcga tctgggcgac gtatttgcgg ctaactttcg tgttgtaggg
     4681 cataagcctc ttggggccag aacggcagaa cttgcaattc cagtcaggtg taacacggga
     4741 aacgcggggt tagttaacgt caacctgagt ctgacggcaa ccacagaccc cagctatccc
     4801 caggcgatta agacgtcacg tcctggcgtg ggcgtggtgg tgaccgatag ccagaacaac
     4861 attatttccc ctgctggtgg aacattaccg ctctctattc ctgatgatgc agacagtatc
     4921 gcgcgaatga atgtctatcc agtcagcacg acaggtgtac caccagaaac cgggcgattt
     4981 gaagccacgg caacggtgag aataaatttt gattaacgcg tgaacgtatg aagaaaaaaa
     5041 cgatatttca gtgcgttatc ttgttcttta gcattcttaa catccatgtc gggatggctg
     5101 ggcctgaaca agttagtatg catatttatg ggaatgtggt cgatcagggc tgtgatgtcg
     5161 ccaccaaaag tgcattacaa aatattcata ttggtgattt taatatcagt gattttcagg
     5221 ccgcgaatac cgtaagcact gctgctgatt tgaatatcga tatcaccggt tgtgccgctg
     5281 gtattactgg cgcggacgtc ctttttagcg gcgaggctga cacccttgcg ccgacactgc
     5341 ttaaactaac tgacacaggc ggaagcggtg gtatggcaac ggggattgcc gtgcaaattc
     5401 ttgatgcgca aagtcagcaa gaaatcccgc tcaatcaggt ccagcctctt acgcccttaa
     5461 aagccgggga taacacactc aaatatcaac ttcgttataa gtccacaaag gcgggagcaa
     5521 cgggcggtaa tgcgacggcg gttctctatt ttgatctggt ttaccagtga aggagtgatg
     5581 ttgaaacgga taatctggat tctgtgctta ctgggattaa cggggagtag tgagttattt
     5641 gctcatgatg gcacggtcaa cattagcgga tcgtttcgtc gtaatacatg cgtgctggca
     5701 caggatagca agcaaatcaa tgtgcagtta ggcgatgtct cactgacacg tttttttcat
     5761 gggaattatg gcccggagaa atctttcatc attaatttgc aggattgcgg aacagatgtc
     5821 agcacggttg acgtcacctt ttcaggaatc cccgacggag tgcagagcga aatgctgtcc
     5881 attgaaagtg gtatggatgc agccagcggg ctggcaattg cgattctgga tgatgcgaag
     5941 atattgattc cgcttaacca ggcgagtaaa gactacagcc tacacagcgg taaggtgcca
     6001 ttaacttttt atgcgcaatt acgacctgtc aatagtgatg tgcagtcggg taaagtgaat
     6061 gccagcgcga catttgtact tcattatgac taatacgtgg aacagattgg cgctcttgat
     6121 ttttgccgtt ttatcgctgc tggtggcggg tgaattgcag gcaggagtgg tggtcggcgg
     6181 aacgcgattt atctttccgg cagacaaaga atcgatagct attttactga ctaataccag
     6241 tcaggaatcc tggcttatta acagtaaaat caatcgccca acgcgttggg ccggggggga
     6301 agtgtcgaca gtgccagcac cattactggc cgctccaccg cttattctcc tgaagcccgg
     6361 tactacgggc acgttgcgct tgctgagaac ggaaagcgac atcttgccag tggatcgcga
     6421 aacgctattt gagttaagca ttgccagcgt gccatccggc aaagttgaaa atcagagcgt
     6481 aaaagtggcg atgcgctcgg tatttaaact gttctggcga cccgaaggtt tgccgggcga
     6541 cccgctggaa gcttatcaac aattacgctg gacacggaat tcgcagggtg tgcaactcac
     6601 taacccaacg ccttactaca ttaacctgat tcaggtgagc gtaaatggta aagcgttaag
     6661 taatgcggga gttgtgccac ctaaaagcca gcgtcagaca agctggtgtc aggtaatagc
     6721 acagtgtcat gttgcctggc gggcgataaa tgattacggc gggttgagtg caaagaagga
     6781 gcaaaatctg ccctgaaaca ggttcggaaa acgtttgcgt tttttttgcc gcaggtcaat
     6841 tcccttttgg tccgaactcg cacataatac gcccccggtt tgcacaccgg gaatccagga
     6901 gagttcatgt actacccctt cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct
     6961 catgagttta cttttcagca attacgccgt attacaggaa cgccgtttga agcactggtg
     7021 cggcagaaag tgcctgcgaa acctgttaac tgcatgggcc tgacgtttaa aaatccgctt
     7081 ggtctggcag ccggtcttga taaagacggg gagtgcattg acgcgttagg cgcgatggga
     7141 tttggatcga tcgagatcgg taccgtcacg ccacgtccac agccaggtaa tgacaagccg
     7201 cgtctctttc gtctggtaga tgccgaaggt ttgatcaacc gtatgggctt taataatctt
     7261 ggcgttgata acctcgtaga gaacgtaaaa aaggcccatt atgacggcgt cctgggtatt
     7321 aacatcggca aaaataaaga tacgccagtg gagcagggca aagatgacta tctgatttgt
     7381 atggaaaaaa tctatgccta tgcgggatat atcgccatca atatttcatc gccgaatacc
     7441 ccaggattac gcacgctgca atatggtgaa gcgctggatg atctcttaac cgcgattaaa
     7501 aataagcaaa atgatttgca agcgatgcac cataaatatg tgccgatcgc agtgaagatc
     7561 gcgccggatc tttctgaaga agaattgatc caggttgccg atagtttagt tcgccataat
     7621 attgatggcg ttattgcaac caataccaca ctcgatcgtt ctcttgttca gggaatgaaa
     7681 aattgcgatc aaaccggtgg cttaagtggt cgtccgcttc agttaaaaag caccgaaatt
     7741 attcgccgct tgtcactgga attaaacggt cgcttaccga tcatcggtgt tggcggcatc
     7801 gactcggtta tcgctgcgcg tgaaaagatt gctgcgggtg cctcactggt gcaaatttat
     7861 tctggtttta tttttaaagg tccgccgctg attaaagaaa tcgttaccca tatctaatta
     7921 tttcttcgaa ttcctcttta taagataatc agggctttat tttcagccct ggttgtttta
     7981 tattcattgc tgttgcttat ttagacattt tgtactttta ttgttgaggt tattaagcga
     8041 agcgacaatg gattgtgttg ctgcggtttt atagggtagg ggagaggcag atgcgaatta
     8101 aaccagacga taactggcgt tggtattacg atgaagagca cgatcgaatg atgctcgatt
     8161 tagccaatgg tatgctattt cgctcacgtt ttgcgcgcaa gatgttgact ccagatgcct
     8221 tgtctcccgc aggcttttgc gttgacgacg ccgcgctcta tttctctttt gaagaaaagt
     8281 gccgcgattt taatttatct aaagaacaaa aagccgaact ggtgctgaat gcactggtag
     8341 caattcgcta tctcaaaccg caaatgccga aaagctggca ttttgtttcc catggtgaaa
     8401 tgtgggtgcc catgccgggc gatgcggcct gcgtttggtt gagcgatact cacgagcagg
     8461 tcaatttgct ggtcgttgaa tctggcgaaa atgccgcact atgcctgctg gcacaaccct
     8521 gcgttgttat tgcgggtcgc gcgatgcagt tgggcgacgc catgaaaatc atgaacgaca
     8581 ggctgaaacc gcaggttaat gttgacagct tcagcctcga acaggcagtc taacgcgcca
     8641 acttaagtgc agtcttcggt acacagctac agcaaagaat ggtgccatca tcgcccattg
     8701 ctgatttttt cagcggcgtg acttcgcctt ctaaaagctg aacacggcaa cttccacaaa
     8761 tgcccgcgcg gcaagaataa gggatacgaa ttccctgatt ttctaattgc tccagcaaca
     8821 cctgttggtt atttccacga aatgcctgtc cctgccagtc aatatctaca tttgcgtccg
     8881 gttgttgcgt gatgttggcg gtatcatcag cggcagctgc gccgtaaatt tttgccggag
     8941 ccgttgccag aatttccacc tcatcgccaa cgcgaatcac gccgctatta cgggcaatta
     9001 aattctgacc aaaatcgaca tcgccgttat cctgggcagt gcggaaagat tgcaatgttt
     9061 ttaatggttc gcctgccgga tgtttttgcc ctttttctgg gctgacggtg gtgaaaatac
     9121 agcggctaca aggtttaacc acatcaaaca ccacatcacc aatgcgaatc actttccagc
     9181 gatcttcttc ccaggctgac gcgccggaaa ccaccagatt ggggcggaat tgctccattt
     9241 ttacactggc cggacaacgt tgttggagat cacgtaacga ggcttcgtta gcaagaaggt
     9301 aaggatagcc atcagcaaat gacagaggta cagtgttgtg gcgtttcacg cgccgggtca
     9361 tttgtggccc cacccagcgt aattgcactt cgcgggagaa aaatccactt agccatttgt
     9421 tgatcgcgtc tggcgcaatt cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt
     9481 cttgtgtggc gaaatcagca aaacgaacat atgcgctact gccatctggt gcggtgagat
     9541 gcaagccatc atgcacaggt gaaggagtaa accgtaccat ctggggaaac tggcgagcgg
     9601 taataaacgt accgtcaggt tccgtgatca taaagatgcg atcgaaggcc agaccactga
     9661 catcagccag agcatgtgta agaccaatgc cgcgcatcga tttaacagga tgaataaaaa
     9721 gccgggttaa tgtcgccacg gtgcggtcct caaatgaaaa taagccctca actttatgac
     9781 atgacgcgct tattagctat aatgcgcaac aattttctta gcgcctgtcc cattaggcta
     9841 ttttatttgt cagtttggcc ctgggttgtg ctcgaaatcc tcacgtactt gtgtacgctc
     9901 cggtttctcc gcgctgtcca tgtccagact gactgcaaca attacgccta ctgcggtagg
     9961 ctcttagagt aaaagtgacg atatgaattc tctgtttgcc agtacggccc gtgggctgga
    10021 agagctgtta aaaactgaac tggaaaacct gggggccgtt gaatgccagg tggttcaggg
    10081 tggggtccat ttcaagggcg acacacggct tgtttaccag agcctgatgt ggagccgcct
    10141 ggcctcgcgt attatgttgc cgctgggcga gtgtaaggtt tacagcgatt tagacctcta
    10201 tctcggtgtt caggcgatca actggacaga gatgtttaat cctggcgcga ccttcgctgt
    10261 ccacttcagt ggtttgaatg acaccatccg caacagtcag tacggcgcga tgaaagtgaa
    10321 agacgcgatc gtcgatgctt tcacgcggaa aaatctgccg cgtccaaatg ttgatcgcga
    10381 tgcgccggat atccgcgtta acgtctggct gcataaagaa accgccagta tcgctcttga
    10441 tctgagtggt gatggtttac atctgcgtgg ctatcgcgat cgtgctggta ttgcgccgat
    10501 caaagaaacc ctggcagccg ctattgtgat gcgatccggc tggcagccag gaacaccgct
    10561 gctcgatccg atgtgtggtt ccggtacgtt gctgattgaa gcggcgatgc tggcgaccga
    10621 tcgcgcacca ggcttgcacc gtgggcgttg gggctttagc ggctgggcgc agcatgatga
    10681 agctatctgg caggaagtga aagcggaagc gcaaactcgc gcccgtaaag gcctggctga
    10741 gtatagctct catttttacg gttcggacag cgacgcacgg gtgattcaac gtgcacgcac
    10801 taacgcccgt cttgcgggga ttggtgaact gatcaccttt gaggtgaaag atgtcgcgca
    10861 actgaccaat ccgctgccga aagggccgta cggcacagtg ttgagcaacc cgccatacgg
    10921 ggaacgtctg gacagcgaac cggcgctgat tgcgctgcat agcctgctgg gtcggatcat
    10981 gaaaaaccag tttggtggct ggaatctctc tttgtttagt gcctcgccgg atctgctaag
    11041 ctgcttgcag ctgcgtgcag acaaacagta caaggcgaaa aacggcccgc tggactgcgt
    11101 acagaaaaat taccacgttg ccgaaagcac tccagacagc aaaccggcga tggtagcgga
    11161 agactacgcc aaccgcctgc gtaagaacct caaaaaattc gagaagtggg ctcgccagga
    11221 aggaattgaa tgttaccgcc tgtatgacgc cgatctaccg gaatataacg ttgccgttga
    11281 ccgttatgcc gactgggtgg tggtgcagga gtatgcgccg ccaaaaacta ttgatgctca
    11341 caaagcgcgt cagcgtctgt tcgatattat cgctgcaacc atttcggtac tggggattgc
    11401 gccaaacaaa ctggtgctga aaacccgtga acgccagaag ggcaaaaatc agtaccagaa
    11461 actgggcgag aagggcgagt ttcttgaagt taccgaatat aacgctcact tgttggtgaa
    11521 cctgacggat tacctcgata ccggtctgtt tctcgatcac cgtatcgccc gtcgtatgct
    11581 cggccagatg agcaaaggca aagatttcct caacctgttc tcttataccg gcagcgccac
    11641 cgtgcatgcg ggattaggcg gtgcacgcag caccaccacc gtggatatgt cgcgtactta
    11701 tctggagtgg gcagaacgca acctgcgtct gaatggcctg accgggcgtg cgcatcgcct
    11761 gattcaggcc gattgcctgg cgtggctgcg tgaggcaaat gaacagttcg atctgatctt
    11821 tatcgatccg ccaaccttct ctaactcaaa acgaatggaa gatgcgtttg atgttcagcg
    11881 cgatcatctg gcgctgatga aagatttgaa acgtctgctg cgtgcaggtg ggacgatcat
    11941 gttctcgaac aacaaacgtg gcttccgtat ggatctcgtc ggcctggcaa aactgggact
    12001 gaaagcacaa gaaattacgc aaaaaacgct ctcccaggat ttcgcccgta accgccagat
    12061 ccacaactgc tggctgatta ccgcagcctg aaaggaatag taatgtcatt aatcagtatg
    12121 catggcgcct ggctgtcgtt cagcgacgcg ccgcttctcg ataacgcaga actgcatatc
    12181 gaagataatg aacgtgtttg tctggtaggc cgcaacggcg caggcaaatc gacgttaatg
    12241 aaaatcctca accgtgaaca agggctggat gacggtcgca ttatttacga gcaagatttg
    12301 attgtagctc gtctgcaaca ggatccgccg cgtaacgttg agggtagcgt ttatgatttc
    12361 gttgccgaag gcattgaaga acaagcggaa tatctgaaac gctatcacga tatttcgcgc
    12421 ctggtgatga acgacccgag cgagaaaaat ctcaacgaat tggcgaaggt tcaggaacag
    12481 ctggatcacc acaacctgtg gcagctggaa aaccgcatca acgaagtgct ggcgcaactg
    12541 gggttagatc ctaacgttgc gctgtcgtcg ctttccggcg gctggttgcg taaagcggca
    12601 ttaggacgcg cgctggtgag taatccgcgc gtgctgttgc ttgatgaacc gacaaaccac
    12661 ctggatattg aaaccatcga ctggctggaa gggtttttga aaactttcaa cgggacgatt
    12721 attttcatct cccacgaccg ttcgtttatc cgcaatatgg cgacgcgcat tgttgatctc
    12781 gatcgcggca agctggtgac ctatctaggg aattacgacc agtacctgct ggaaaaagaa
    12841 gaagccctgc gcgtggaaga attacaaaat gccgagttcg atcgcaaact ggcgcaggaa
    12901 gaggtgtgga tccgccaggg gatcaaagca cgccgtaccc gtaatgaagg ccgcgtacgc
    12961 gccctgaaag cgatgcgtcg cgagcgtggt gaacgtcgcg aagtgatggg taccgcaaag
    13021 atgcaggtgg aagaggccag ccgctccggt aagatcgttt tcgaaatgga agacgtttgc
    13081 taccaggttg acggtaagca actggtgaaa gatttttcgg cccaggttct acgtggcgac
    13141 aaaattgccc tgattggtcc gaatgggtgc ggcaaaacca cgctgctgaa actgatgctc
    13201 ggtcagcttc aagcggacag cgggcgtatt cacgttggca ccaaactgga agtggcttat
    13261 ttcgatcagc accgcgcgga actggatccc gataaaacgg tgatggataa ccttgccgaa
    13321 ggtaagcaag aggtgatggt taacggcaag ccacgctacg tattgggcta tttgcaggac
    13381 tttctgttcc atccgaaacg ggcgatgacg ccggtacgtg cgctttctgg cggtgagcgg
    13441 aaccgcttgc tgctggcgcg tttgttcctc aaaccaagca acttattgat tcttgacgaa
    13501 ccgaccaacg atcttgatgt cgaaacgctg gaactgctgg aagaactgat cgacagctat
    13561 cagggcacgg tattgctggt tagccacgat cgtcagtttg tcgataacac cgttacagaa
    13621 tgttggatct tcgaaggcgg cggtaaaatt ggtcgttatg tcggcggtta tcatgatgcc
    13681 cgtggtcagc aagagcagta tgtggcgctc aaacagcctg cggtgaaaaa aaccgaagaa
    13741 gccgccgcgg caaaagcaga aactgtaaaa cgcagcagta gcaaactaag ctataaattg
    13801 cagcgcgaac tggagcagct accgcaattg ctcgaagatc tggaggcgaa gctggaagcc
    13861 ctacagacgc aggtggcgga tgcttccttc ttcagtcagc cacatgagca gacacaaaaa
    13921 gtgctcgccg atatggctgc tgcagagcag gagctggagc aagcctttga acgctgggag
    13981 tatcttgaag cgttaaaaaa tggtggctga gcgcaaaata gtcgatttaa acagagacag
    14041 ctgacaacgg taaaattgcc tgatgaactg ggtttatcag gcatgaaaac caacactttg
    14101 ttagtcatct gtaaaatagc gcatcattaa ggagtaccaa tgtgcgaaca tcatcatgcc
    14161 gcgaagcaca tcctgtgctc gcagtgtgac atgctggtgg cgttaccgcg ccttgagcat
    14221 ggtcagaaag cggcatgtcc ccggtgtggc acaacgttaa ccgtggcgtg ggatgcccct
    14281 cggcagcgtc ccaccgccta tgcgttggct gcactgttca tgctgttgct gtccaacttg
    14341 tttccttttg tgaatatgaa cgttgcagga gttaccagtg aaattacatt actggaaatt
    14401 cccggcgtgc ttttttctga ggactacgcc agcctcggca cctttttcct gttgtttgtg
    14461 caactggttc ccgcgttttg tctgataacc attctgttac tggtgaatcg cgcggaatta
    14521 ccggtccgtt taaaagagca actggcacgg gtgctttttc aactcaaaac ctggggaatg
    14581 gcggagattt tcctcgctgg tgtgctggtc agtttcgtta aactgatggc ttacggcagc
    14641 attggggtag gcagcagctt tctcccctgg tgtttatttt gtgtcctgca actgcgcgct
    14701 tttcagtgcg ttgatcgtcg ctggttatgg gacgacatcg ccccgatgcc agaactgcgc
    14761 cagccgctaa aaccaggcgt cacggggata cgtcaggggc tgcgttcgtg ctcctgttgt
    14821 acggcaatcc ttcctgctga tgaacccgtg tgcccgcgtt gtagtaccaa agggtacgtt
    14881 cggcgtagaa acagcctgca gtggacactc gcgctgcttg taacgtccat catgctgtat
    14941 cttccggcta atattttgcc catcatggtg acggatttat taggctcgaa gatgccgtcg
    15001 acgattctcg ctggggtcat tctgttatgg agcgaaggct cttatcccgt cgctgcggtg
    15061 atctttctgg ccagtattat ggtgccaacg ttaaagatga tcgccatcgc gtggctgtgt
    15121 tgggatgcca aagggcatgg caagcgcgac agtgaaagaa tgcatttgat ttatgaagtt
    15181 gttgagtttg taggccgctg gtcgatgatt gacgttttcg ttatcgcggt gctctcggcg
    15241 ctggtgcgta tgggaggttt aatgagtatt tatccggcaa tgggtgcatt aatgtttgct
    15301 ttagtcgtca taatgacaat gttttctgct atgacgtttg acccgcgttt gtcgtgggat
    15361 cgtcaacctg aatcagagca tgaggagtcc tgacagtatg gaatctaata atggggaagc
    15421 caaaatccag aaagtgaaga actggtctcc cgtgtggata tttcctatcg tcacggcgct
    15481 cattggggcc tgggttcttt tttatcatta cagccatcag ggaccggaag tgaccctgat
    15541 caccgcgaat gcggaaggaa ttgaaggtgg caaaaccacc attaaaagcc gtagcgttga
    15601 cgtcggcgtg gttgaaagcg ccacactggc tgatgatttg acgcacgttg aaatcaaagc
    15661 gcggctgaat tccggtatgg aaaaattgct gcataaagac accgtctttt gggtggtgaa
    15721 accgcagatt ggtcgcgaag ggattagcgg cctgggaacg ctgctgtctg gagtttatat
    15781 cgaactgcag ccaggcgcga aaggcagcaa aatggataaa tacgatttgc tggactcgcc
    15841 accgttggcc ccgcctgatg cgaaaggtat ccgtgtgatt ctcgatagca aaaaagccgg
    15901 gcagctctcg ccaggagatc cggtgctgtt ccgtggctat cgggtaggtt cggttgaaac
    15961 cagcaccttc gatacacaaa aacgcaatat cagctatcaa ctgttcatca atgcacctta
    16021 tgaccgactg gtgaccaaca atgttcgctt ctggaaagat agtggcattg cggttgatct
    16081 gacgtcagca gggatgcgtg tggagatggg ctcattgaca acgctgctga gtggcggtgt
    16141 cagctttgat gtgccggaag gtctggattt agggcagcca gtggcaccga aaacagcttt
    16201 cgttttgtat gatgatcaga agagcattca ggattcgttg tacaccgatc acattgatta
    16261 tctgatgttc tttaaagatt cggtacgcgg tctgcaaccg ggagctccgg tagagttccg
    16321 gggtattcgc ctgggtaccg taagcaaagt gccattcttt gcgccgaata tgcgtcagac
    16381 atttaacgat gattaccgta ttccggtact gattcgtatc gagccagagc ggctgaaaat
    16441 gcagcttggc gaaaatgcgg atgttgttga gcaccttggc gaattgttga aacgtggttt
    16501 acgcggatcg ctgaaaaccg gaaacctggt cactggtgca ctgtatgttg atctcgattt
    16561 ctatccaaat acgcctgcaa taaccggtat tcgtgaattt aatggttatc agattatccc
    16621 gaccgttagc ggcggcctgg cgcaaatcca gcaacgactg atggaagcgt tggataagat
    16681 caacaaactg ccattgaatc cgatgattga acaggcaacc agtacgcttt atgaaagtca
    16741 gcgcacaatg aaaaacctgc aaacgacgct ggatagcatg aacaagatcc tcgctagcca
    16801 gtcgatgcag cagttgccga cggatatgca gtcaacgttg cgtgaattga atcgcagcat
    16861 gcagggcttc cagcctggct ccgcagccta caacaagatg gtggcggata tgcagcgcct
    16921 tgatcaggtg ttgcgagaac tgcaaccggt gctgaaaacg ctcaatgaga agagtaacgc
    16981 gctggtattt gaagcgaagg acaaaaaaga tccagagccg aagagggcga aacaatgaaa
    17041 aagtggctag tgacgattgc agcactgtgg ctggccggat gcagctccgg cgaaattaat
    17101 aaaaactatt accagttacc tgtggtgcag agcggtacac aaagtaccgc cagccagggc
    17161 aatcgtctgt tatgggtaga gcaggtcact gttcctgact atctggcggg gaatggtgtg
    17221 gtttatcaaa ccagtgatgt gaagtatgtg attgccaaca acaacttgtg ggccagcccg
    17281 ttggatcaac agttgcgcaa caccctggtt gccaacctga gtacgcaact gcccggctgg
    17341 gtggttgcct cccagcctct gggaagcgcc caggacacgc tcaatgttac cgtaacggag
    17401 tttaacggtc gctatgatgg caaggtcatt gtcagtggtg agtggctgtt gaaccaccag
    17461 ggacaactga tcaaacgtcc gttccgtctg gaaggagtgc aaactcagga tggttacgat
    17521 gagatggtta aagtgctggc cggtgtctgg agtcaggaag ccgcttctat tgcacaagag
    17581 ataaagcgtc taccttaatt ataaagattt gtaaatataa ccgtctccgg tatgttgcct
    17641 gaggcggttt ttttgtctct aacgtgcgga aaaatttgtt cctcttcaca ttttttgtac
    17701 aaccgacatg cccgtgtagc tcacaaatat gacagtggcg tgaattttgc gcattgacgg
    17761 cagttatgat tcgcggtatt gcttaactgt gattgcacat ttagtaatca ctgttttctt
    17821 ttccaccaga aaccagtatg agggaaacga ggcatgaaga gacaaaaacg agatcgcctg
    17881 gaacgggcac atcaacgtgg ttatcaggcc ggcatcgccg gacgctcaaa agaaatgtgt
    17941 ccctatcaga cgctgaatca aaggtcacaa tggctgggag gctggcgaga agccatggcg
    18001 gacagggtag taatggcctg attctgtctc tttaaaaaga aacctccgca ttgcggaggt
    18061 ttcgcctttt gatactctgt ctgattataa tcagaaggca gacgtatcct ggaacagacc
    18121 gactttcagg tcgctggcgg tatagatcag acgaccatca accagcactt cgccatccgc
    18181 caggcccata atcagacgac ggttaacaat gcgtttaaag tgaatacggt aggtcacttt
    18241 tttcgctgtc ggcagtacct gaccagtgaa tttcacttcg ccaacgccca acgcgcggcc
    18301 tttaccttcg ccgcccagcc agccgaggta gaaccctacc agctgccaca ttgcgtccag
    18361 gcccaggcat cccggcataa ccggatcgcc aataaagtgg catccgaaga accacagatc
    18421 cggattgata tccagttctg cttcaacata ccctttgtcg aagttaccac ccgtttcggt
    18481 cattttgacc acacggtcca tcatcagcat gttcggtgct ggcaattgcg ggcctttagc
    18541 gccaaacagt tcaccgcgac cagaggcaag aaggtcttct tttgtatagg attcgcgttt
    18601 atctaccatg ttctctgtaa gccttatttt attgaagcac gcaggatagc taacacgtgt
    18661 acgctgaaca agtccgatca gttcggaata aaccagttca gccaacgtaa tggccatgga
    18721 aaacggtgac gtccttcctg ttgcgatgct tgcgcgatac gttcctggat ggtttgcatc
    18781 agcgtcgttt ggccttcgcc atcccacacc agatttaata ataacggcag tgcgtcagtc
    18841 acatcgtcta ctgcccagat ggtgaatttg ccttcttcta ccgctttcac cagttcactg
    18901 tgaagactta aatggcgaac gttagctgtg gggataatga caccttgttt cccggttaac
    18961 tcacgttgct ggcaaatagc aaagaagcct tcgattttct catttaaacc accgaccggc
    19021 tgggcgcgac cgaactgatc gactgaacct gtgatagcga tactctgatt caccggcaca
    19081 tcggcgaggg cgcttatcag ggcgcagagt tcagccatcg aggcactatc tccatcaact
    19141 tcactgtatg actgctcaaa tgtcagcgat gctgagaagg ggatctgttg ctcaagctgt
    19201 agttccgaca tcaggaacgc ttgcatgatc atcatccctt tcgcatggat attgccgcca
    19261 agctccgctt tgcgttcgat gtcggtgaat tcaccatcgc caatatgcac aacgcagcta
    19321 atgcgagaag gttcgccaaa agcgcgtgga tgacccggaa attcaatgac cgaaagggcg
    19381 ttaatttgcc cgatgcgttc gccttcggtt tcaatcagga tttgctcctg aaggatctca
    19441 tcctgcatac gttcagcgag gaaaccttcg cgccattcac gctgctgcag cattaagttt
    19501 agctgctcgc cggagaaggt gtcgccatca cacagggagg cgacctcttt acactggcgg
    19561 aggatccact gcgggctaag cggaagtgtt tcttgttcac cggtgtagcg tgctgcttcg
    19621 cggataagta ccggccaggc atccgctccc ggtgcaggta agtgattatg tctggcggta
    19681 aatgtcaccc agcgacacca ctgggttact gactccgcat cgacaatctg cagagtatct
    19741 tcaaattcgc tataaatagc ctgctctgaa agctctggct ccatctcctg gaaatcagcc
    19801 aatgattcgc gttcgcctac cagaatgact ttcagcttca atggcatcga aggcacagag
    19861 acggggagag ggcgcgactc atcaaacgca acccagtcaa aacgctcgcg gttaacgata
    19921 tttttcagcc gcatccacag cagaggttgc gccagcagtg tacgcaaaga gatgatgaga
    19981 ataccgccat ttgcctgatg caccagacca ggctgcaggg taatgtcgcc attaaactgg
    20041 cgcaggcagc caaagagttg ctccgcttct acccagtcgg cagcgacaac ttgcgttaaa
    20101 gtcgcaaaat tatcatctgc actcactgcg tgacgtaagc ggatggagtg gccggaaacc
    20161 tcatagtgac cgcccaccag ttggcctgca tcgctttgta gcgtacgcgc ggcattggca
    20221 ataagattca gatactcaga ctcttccggg gccttcgcca gcataaaaga ggaggatgct
    20281 cgcgtatgca gcaactgctc cagcgcaaat tgcagccgcg gttgagtatc actgaataaa
    20341 ggatcgtttt cgtcaatcaa atgtggctga gcaaatattt cctgatagct atcggtatca
    20401 ggaaccaggt cacgccatgc aagtttcgta atggtcaaag ttgatgtttt ttagtctgtt
    20461 gtcaaagccg cgattatacc gtaaccggca ctacagcaca cgtagaaagc accgacaata
    20521 ctcctggcat gggcgttaaa gctcacagga tggagattct tttcttcact ggcctaaaaa
    20581 gctgatattc tgtaaagagt tacacggtaa cattgagatc gctatgaaat atcaacaact
    20641 tgaaaatctt gaaagcggtt ggaaatggaa gtatctggtg aagaagcatc gcgaagggga
    20701 gttaatcacc cgttacatag aagccagtgc cgcccaggaa gccgtcgatg tgttgctctc
    20761 gctggaaaat gaacccgtgc tggtaaatgg ctggattgac aagcatatga atccggaact
    20821 ggtcaatcgc atgaagcaaa ccattcgggc cagacggaag cgccatttta atgcggaaca
    20881 ccagcatacg cgcaaaaaat cgatcgatct ggaatttatt gtctggcaac gtctggctgg
    20941 tcttgcgcag cgtcgcggta aaacgctttc tgaaacgatt gttcagctga ttgaagatgc
    21001 ggaaaacaaa gagaaatacg cgaataaaat gtcttctctg aagcaggatc tgcaggcatt
    21061 gctgggtaag gaataactga cgaaagtcag ttcaatttac taaaggcaaa aaaaaccccg
    21121 cagcagcggg gtttttctac cagacgagaa cttaagcttg cggctgagtt acaacgtctt
    21181 tgataccttt aacttcgatc tctacgcgac gatccggagc caggcagtcg atcagtgcag
    21241 cacgctgttt cacgttgtca caggtgttgc cagtaaccgg gttggattcg cccataccac
    21301 gtgcggagat cttgtctgcc gggatacctt tggagatcag gtaatcaaca acagactgag
    21361 cacggcgctc ggacagaccc tggttgtaag cgtcagaacc gatgcggtcg gtgtaaccca
    21421 gaacaactac ggaaccgtct ttcggatcca ggttgctcag ctggctgtac agctgatcca
    21481 gagcagcctg accttccggt ttcagggttg ctttgttgaa gttgaacaga acgtcagact
    21541 tcagagtgaa gtgcttggtc tgtacttccg gtgccggagc tggagccgga gcaactactg
    21601 gagctgcttc gccctgaccg aaacggtagg aaacacccag gctcagcatg ccgttgtccg
    21661 gacgagtgcc gatggtgtgt gcgtcaccga tgttgttggt ccactggtat tccagacggg
    21721 tagcgatttc aggagtgatc gcgtactcaa caccacctgc gaaaaccgga gaaacgccag
    21781 tgtcgtggtc tttaaaggat gcgccaccag gtacgttagc cttggtgtct gcacgccata
    21841 ccataccacc cagacgagtg tatacgtcca gatcgtcagt gattgggtaa cccagtttag
    21901 cagtcagttg aacgccctga gctttgtatg caccgttgat gttgtcgcct ttgtacggca
    21961 tacgacctaa ccagtcgtaa cccatttcaa aaccaacata cgggttaacc tggtaaccac
    22021 caaaagcacc tgcacccagt tggttttcgt gggtcggacc attgttagga ataaaaccag
    22081 tgtcatggta ctgggaccag cccagtttag caccagtgta ccaggtgtta tctttcggag
    22141 cggcctgcgc tacggtagcg aaaccagcca gtgccactgc aatcgcgata gctgtctttt
    22201 tcattttttg cgcctcgtta tcatccaaaa tacgccatga atatctccaa cgagataaca
    22261 cggttaaatc cttcaccggg ggatctgctc aatattaact ctaccgatat cttcggctta
    22321 tgccgagcac ccctggcgat gtaaagtcta caacgtagtt gaaaacttac aagtgtgaac
    22381 tccgtcaggc atatgaaaaa aaagtcttgt ataaggtatg tttaatcttt tttgtcagcg
    22441 acaatttaca ga
//
LOCUS       DAFNBJ010000076        22447 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000076 DAFNBJ010000000
VERSION     DAFNBJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.076"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..132)
                     /locus_tag="L2X49_002727"
     CDS             complement(<1..132)
                     /locus_tag="L2X49_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBN4797795.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     "
     gene            complement(142..693)
                     /gene="ybcL"
                     /locus_tag="L2X49_002728"
     CDS             complement(142..693)
                     /gene="ybcL"
                     /locus_tag="L2X49_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBN4797796.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(862..1194)
                     /gene="emrE"
                     /locus_tag="L2X49_002729"
                     /pseudo
     CDS             complement(862..1194)
                     /gene="emrE"
                     /locus_tag="L2X49_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
     gene            complement(1528..1842)
                     /gene="fliE"
                     /locus_tag="L2X49_002730"
     CDS             complement(1528..1842)
                     /gene="fliE"
                     /locus_tag="L2X49_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBN4797797.1"
                     /translation="MSAIQGIEGVISLLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKVSVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2057..3715
                     /gene="fliF"
                     /locus_tag="L2X49_002731"
     CDS             2057..3715
                     /gene="fliF"
                     /locus_tag="L2X49_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBN4797798.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3708..4703
                     /gene="fliG"
                     /locus_tag="L2X49_002732"
     CDS             3708..4703
                     /gene="fliG"
                     /locus_tag="L2X49_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBN4797799.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4696..5382
                     /gene="fliH"
                     /locus_tag="L2X49_002733"
     CDS             4696..5382
                     /gene="fliH"
                     /locus_tag="L2X49_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBN4797800.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQIALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5382..6755
                     /gene="fliI"
                     /locus_tag="L2X49_002734"
     CDS             5382..6755
                     /gene="fliI"
                     /locus_tag="L2X49_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBN4797801.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGTETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6774..7217
                     /gene="fliJ"
                     /locus_tag="L2X49_002735"
     CDS             6774..7217
                     /gene="fliJ"
                     /locus_tag="L2X49_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBN4797802.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7214..8341
                     /gene="fliK"
                     /locus_tag="L2X49_002736"
     CDS             7214..8341
                     /gene="fliK"
                     /locus_tag="L2X49_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBN4797803.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQHSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8446..8910
                     /gene="fliL"
                     /locus_tag="L2X49_002737"
     CDS             8446..8910
                     /gene="fliL"
                     /locus_tag="L2X49_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBN4797804.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8915..9919
                     /gene="fliM"
                     /locus_tag="L2X49_002738"
     CDS             8915..9919
                     /gene="fliM"
                     /locus_tag="L2X49_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBN4797805.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9916..10329
                     /gene="fliN"
                     /locus_tag="L2X49_002739"
     CDS             9916..10329
                     /gene="fliN"
                     /locus_tag="L2X49_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBN4797806.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10332..10697
                     /gene="fliO"
                     /locus_tag="L2X49_002740"
     CDS             10332..10697
                     /gene="fliO"
                     /locus_tag="L2X49_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBN4797807.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            10697..11434
                     /gene="fliP"
                     /locus_tag="L2X49_002741"
     CDS             10697..11434
                     /gene="fliP"
                     /locus_tag="L2X49_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBN4797808.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11444..11713
                     /gene="fliQ"
                     /locus_tag="L2X49_002742"
     CDS             11444..11713
                     /gene="fliQ"
                     /locus_tag="L2X49_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBN4797809.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVSTLFTNLPYIIG"
     gene            11721..12506
                     /gene="fliR"
                     /locus_tag="L2X49_002743"
     CDS             11721..12506
                     /gene="fliR"
                     /locus_tag="L2X49_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBN4797810.1"
                     /translation="MLQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLTLLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12796..13419
                     /gene="rcsA"
                     /locus_tag="L2X49_002744"
     CDS             12796..13419
                     /gene="rcsA"
                     /locus_tag="L2X49_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBN4797811.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13463..13651)
                     /gene="dsrB"
                     /locus_tag="L2X49_002745"
     CDS             complement(13463..13651)
                     /gene="dsrB"
                     /locus_tag="L2X49_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBN4797812.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13814..14041
                     /gene="yodD"
                     /locus_tag="L2X49_002746"
     CDS             13814..14041
                     /gene="yodD"
                     /locus_tag="L2X49_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBN4797813.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14339..15157
                     /locus_tag="L2X49_002747"
     CDS             14339..15157
                     /locus_tag="L2X49_002747"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HBN4797814.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQQLSGKRPTTLGLGDGPN
                     DAPLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15154..16848)
                     /gene="yedQ"
                     /locus_tag="L2X49_002748"
     CDS             complement(15154..16848)
                     /gene="yedQ"
                     /locus_tag="L2X49_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HBN4797815.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDIAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17019..17201)
                     /locus_tag="L2X49_002749"
     CDS             complement(17019..17201)
                     /locus_tag="L2X49_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBN4797816.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17280..18197)
                     /locus_tag="L2X49_002750"
     CDS             complement(17280..18197)
                     /locus_tag="L2X49_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBN4797817.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            18370..19290
                     /gene="yedA"
                     /locus_tag="L2X49_002751"
     CDS             18370..19290
                     /gene="yedA"
                     /locus_tag="L2X49_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBN4797818.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19279..19749)
                     /gene="vsr"
                     /locus_tag="L2X49_002752"
     CDS             complement(19279..19749)
                     /gene="vsr"
                     /locus_tag="L2X49_002752"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBN4797819.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19730..21148)
                     /gene="dcm"
                     /locus_tag="L2X49_002753"
     CDS             complement(19730..21148)
                     /gene="dcm"
                     /locus_tag="L2X49_002753"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBN4797820.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21215..21910)
                     /locus_tag="L2X49_002754"
     CDS             complement(21215..21910)
                     /locus_tag="L2X49_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBN4797821.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSSAG"
     gene            complement(21950..22315)
                     /locus_tag="L2X49_002755"
     CDS             complement(21950..22315)
                     /locus_tag="L2X49_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBN4797822.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         5475 a         5899 c         5715 g         5358 t
ORIGIN      
        1 ccaattgata actttttttc cctgacggat acgacataaa gctggcataa gaggcttaac
       61 cctgtgaatc tcatgatgtt tatgcatccg tatttcttca atctttaggt tagtcctatc
      121 ttttgccagc atactcttat cctactttac ctcataaacg ggtattatct cagcggttgc
      181 aattttattg gcattaagca tataaccaac taacgctccg ctggaattag aatccacagg
      241 aatcttatca gtttttagag cccatatttt aaactggtaa tgatggggtt tatctccttt
      301 gggaggacat gctccaccaa atcccgcata gccaaaatca tttcggcctt ggacagcacc
      361 agtcggaagt tttgtaccat cacgtcttcc tgcatcagcg ggcaaatatg ttaccgttgc
      421 tggaatatta gcaacagtcc agtgccacca tccgctgcct gtaggcgcat ctggatcata
      481 cacagttacg gcaaagcttt tggtgccttc tggagcacca gaccaggtta atgagggcga
      541 tgtattaccc ccctcacacc caaatccaga aaatacatga gacgttgtaa gttgctctcc
      601 tgtttttatt tcattactag tgacctgaaa tgctgcggcc tgcgcagaaa atgttatgaa
      661 tgccaataca cttgaaacga taagtttttt cataaaaacc tctttgttgt gacctatcgt
      721 tattttattt gatgtgcatt gatctcatta tgcaaaaagg cgcaatgatc gcgcaaatgc
      781 aatcataatt ataaagttgc cccgaacatt agccataata tgcacaaatc agattcctgt
      841 tatctcaagg atgattattt tttaattcgg tgcgcttcgt gacaacaagt taatgactaa
      901 cacaccggca caaatcaaca tcatgcctat gatggctggc aggtccagtc gttggccaaa
      961 aaatccccag gaaagcaaac taatcagaac gataccgact cctgaccaga tagcataagc
     1021 aatccctgta ggtatataag ccagtgtctg agctaataac caaaatgatg cacaatagca
     1081 aataattgtg ccaacagagg gctataaccg tgtaaacccc tctgaaaact tcattaaggt
     1141 tgtaccaatg acctctgcaa gtattgcgcc accaagataa atataagggt tcatagcata
     1201 ttctttcctg ttcatactgg agagaattgt actacagttt gaacacaact caccgttttc
     1261 atcctggtgt atgtgtatcc agggggctct ggcgtggcaa gagataaagg caggaacaat
     1321 aatgatcaga aggaacccca ggctaacaaa agagttcact cttcctatga gcaaagcgaa
     1381 gacaaaacat attatcgcgt ttacggatcg tggatggcag aaaactttca ggatcaggta
     1441 tccattttaa accagaaatt aagtgagttt gccccatcca tgccccacgc gggcagatgg
     1501 gatgtaatta acaacaggtt acaaaaccta cacctgcatg gacatcactt cctgatacgc
     1561 cgccaccagc ttattacgca cctgaatccc catttgcata gaaactgaga ctttttgcat
     1621 atcggtcatc acatcgttta acgccacgcc gggttcaccg agggtgaatt tttctgcctg
     1681 cgtgcgggca gctgtttgtg tatcgctaat gcgatcgagc gcggcgtgca gctgcccggc
     1741 aaaactaatg gtcggttgcg gcagtgaatc ctgcgcacgc gcactcatcg ccgtagcctg
     1801 taacaggctg ataacccctt caatcccctg tatcgctgac attttcgtct cctggataat
     1861 ttctggtagc aaagcctacc agtaagtcaa taagacaaag gcgctaaata gcaacaaaaa
     1921 aacgggttta ttggcggata gaaaaaaacg aaagcacaaa taatggcagc gtcaattttt
     1981 cgagtttgct gacccgggag tgagtcttgt tccactttgc caataacgcc gtccataatc
     2041 agccacgagg tgcgcgatga atgcgactgc agcccagaca aaatctcttg agtggcttaa
     2101 tcgcctgcgt gcgaatccga aaattccatt gattgttgcc ggttccgcgg cagtggcggt
     2161 catggtcgca ctgatcctgt gggcgaaagc ccccgactac cgcacattat tcagcaatct
     2221 ttccgatcag gatggtggcg caattgtcag ccaactgacg caaatgaata ttccttaccg
     2281 cttcagcgaa gccagcggcg ctattgaagt tccggcagat aaagttcacg aactgcgtct
     2341 gcgcctggca caacaaggtt tgcccaaagg cggcgcggtc ggtttcgaac tactcgatca
     2401 ggaaaagttt ggtatcagcc agttcagcga acaggtgaat tatcagcggg cgctggaagg
     2461 cgagctttct cgtaccatcg caactatcgg cccggtaaaa ggggcgcgcg tacatctggc
     2521 aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc ccttctgcat cggtgacggt
     2581 aaatctgtta cccggccgcg cactcgatga agggcaaatt agcgccattg tgcatctggt
     2641 ttccagcgcc gttgctggtc tgccgccggg aaacgtcacg ctggtggatc agggcggaca
     2701 tctgttaacc cagtccaata ccagcgggcg cgatcttaat gatgctcagt tgaaatatgc
     2761 cagcgatgtc gaaggccgta ttcagcggcg tattgaagca atcctgtcgc ctattgttgg
     2821 taacggtaat attcacgccc aggtcacggc gcagctggac ttcgccagta aagaacaaac
     2881 ggaagaacag tatcgcccta acggtgatga atctcatgcg gcgcttcgtt cacgccagct
     2941 taatgagagc gagcaaagcg gttccggtta tccgggcggc gtaccggggg cgttgtcgaa
     3001 tcaaccggca cctgcgaata acgcgccaat cagcacgcct ccggcaaatc aaaataaccg
     3061 ccagcagcag gcgagtacca ccagcaatag cgggccgcgt agcacacagc ggaatgaaac
     3121 cagtaactac gaagtcgatc gcaccattcg tcataccaaa atgaacgtgg gcgatgtgca
     3181 acgtctgtca gtcgcggtag tggtgaatta caaaaccttg ccagacggta aaccattgcc
     3241 tctcagcaac gaacagatga agcaaattga agatctgacc cgcgaggcga tgggcttttc
     3301 tgaaaaacgc ggtgactcgc tcaatgtcgt taactcgccg ttcaatagca gtgacgaaag
     3361 cggcggagaa ctgccgttct ggcaacagca agcgtttatc gatcagttgc tggctgccgg
     3421 tcgctggttg ctggtactgc tggtggcgtg gctgctgtgg cggaaagcgg tacgcccgca
     3481 gttaacacgt cgcgccgagg cgatgaaagc tgtacagcaa caggcgcagg cccgtgagga
     3541 agtggaagat gcggtggaag tccgcctgag caaagacgaa caactccaac aacgacgcgc
     3601 taaccaacgt ctgggggcag aagtcatgag ccagcgtatc cgtgaaatgt ctgataacga
     3661 tccgcgcgtg gtggcgctgg tcattcgcca gtggataaat aacgatcatg agtaacctga
     3721 caggcaccga taaaagcgtc atcctgctga tgaccattgg cgaagaccgg gcggcagagg
     3781 tgttcaagca cctctcccag cgcgaagtgc aaaccctgag cgctgcaatg gcgaacgtca
     3841 cgcagatctc caacaagcag ctaaccgatg tgctggcgga gtttgagcaa gaagctgaac
     3901 agtttgccgc actgaatatc aacgccaacg attatctgcg ttcggtattg gtcaaagctc
     3961 tgggtgagga acgtgccgcc agcctgctgg aagatattct cgaaactcgc gataccgcca
     4021 gcggtattga aacgctcaac tttatggagc cgcagagtgc cgccgatctg attcgcgatg
     4081 agcatccgca aattatcgcc accattctgg tgcatctgaa gcgcgcccag gccgccgata
     4141 ttctggcgtt gttcgatgaa cgtctgcgcc acgacgtgat gttgcgtatc gccacctttg
     4201 gcggcgtgca gccagccgcg ctggcggaac tgaccgaagt actgaatggc ttgctcgacg
     4261 gtcagaatct caagcgcagc aaaatgggcg gcgtgagaac ggcagccgaa attatcaacc
     4321 tgatgaaaac tcagcaggaa gaagccgtta ttaccgccgt gcgcgaattc gacggcgagc
     4381 tggcgcaaaa aattatcgac gagatgttcc tgttcgaaaa tctggtggat gtcgacgatc
     4441 gcagcattca gcgtctgttg caggaagtgg attccgaatc gctgttgatc gcgctgaaag
     4501 gggccgagca gccactgcgc gagaaattcc tgcgcaatat gtcgcagcgt gccgccgata
     4561 ttctgcgcga cgatctcgcc aaccgtggtc cggtgcgtct gtcgcaggtg gaaaacgaac
     4621 agaaagcgat tctgctgatt gtgcgccgcc ttgccgaaac tggcgagatg gtaattggca
     4681 gcggcgagga tacctatgtc tgataatctg ccgtggaaaa cctggacgcc ggacgatctc
     4741 gcgccaccac aggcagagtt tgtgcccatg gtcgagccgg aagaaaccat cattgaagag
     4801 gccgaaccca gccttgagca gcaactggcg caactgcaaa tgcaggccca tgagcaaggt
     4861 tatcaggcgg ggattgccga aggtcgccag caaggtcatg agcagggcta tcaggaagga
     4921 ctggcccggg ggctggagca aggtctggca gaggcgaagt ctcaacaagc gccaattcat
     4981 gcccggatgc agcaactggt cagcgaattt caaactaccc ttgatgcgct tgatagtgtg
     5041 attgcttcgc gcctgatgca gatagcgctg gaggcggcac gtcaggtgat cggtcagacg
     5101 ccaacagtgg ataactcggc actgatcaaa cagatccaac agttgttgca gcaagaaccg
     5161 ttattcagcg gtaaaccaca gctgcgcgtg cacccggatg atctgcaacg tgtggatgat
     5221 atgctcggcg caaccttaag tttgcatggc tggcgcttgc ggggcgatcc cacactccat
     5281 cctggcggct gtaaagtctc cgccgatgaa ggcgatctcg acgccagtgt cgccactcgc
     5341 tggcaagaac tctgccgtct ggcagcacca ggagtggtgt aatgaccacg cgcctgactc
     5401 gctggctaac cacgctggat aactttgaag ccaaaatggc gcagttgcct gcggtacgtc
     5461 gctatgggcg attaacccgc gctaccgggc tggtgctgga agccaccgga ttacaattgc
     5521 cgctcggcgc aacctgtgtc attgaacgcc agaacggcac tgaaacgcac gaagtagaaa
     5581 gcgaagtcgt tggctttaac ggtcaacggc tgtttttaat gccgctggag gaagtcgaag
     5641 gtgtcctgcc cggcgcgcgg gtttatgcca aaaacatttc ggctgaagga ctgcaaagcg
     5701 gcaagcagtt gccgctcggt ccggcgttat taggtcgcgt tctggacggc agcggtaagc
     5761 cgctcgatgg cctgccctcc cccgatacga cggaaaccgg tgcgctgatt accccgccat
     5821 ttaacccgtt gcaacgtaca ccgattgaac atgtgctgga caccggcgtg cgcccaatca
     5881 atgccctgct taccgttggg cgtgggcagc gtatggggct gtttgccggg tctggcgttg
     5941 gtaaaagtgt gctgctgggg atgatggccc gttacacccg cgccgatgtc attgtcgtgg
     6001 gtttgattgg tgaacgtggg cgcgaagtaa aagattttat tgagaatatc ctcggtgccg
     6061 agggacgtgc acgctccgtg gtgattgccg ctccggcgga tgtctctccg cttctgcgaa
     6121 tgcaaggtgc cgcctatgcc acgcgaattg ccgaagattt tcgcgatcgt ggtcagcatg
     6181 tgttgctgat tatggactcc ctcacccgct acgcgatggc ccagcgtgaa attgcgctgg
     6241 cgattggcga accacctgcc actaaaggtt atccaccgtc ggtgtttgcc aaattaccgg
     6301 cactggtcga gcgtgccgga aatggcatta gcggcggcgg atcgattact gcattttata
     6361 ccgtgctcac tgaaggcgat gaccagcagg accccattgc cgactctgcg cgggcaatcc
     6421 tcgacggtca cattgtgctg tctcgccgac tggcggaagc cgggcactat ccggctatcg
     6481 atattgaagc gtcgatcagc cgcgcaatga cggcgttgat cagtgagcaa cattacgcgc
     6541 gagtgcgcac cttcaaacag ctgttgtcga gttttcagcg taaccgcgat ctggttagcg
     6601 tcggcgcgta tgccaaaggc agcgatccga tgctcgacaa agccatcgcc ctgtggccgc
     6661 agctggaggg ctatttgcaa caaggcattt ttgaacgcgc ggactgggaa gcgtctctcc
     6721 aggggctgga gcgtattttc ccgacagtgt cataacccag gagataacgg cagatggcag
     6781 aacatggcgc gctggcgacc ctgaaagatc tggcagaaaa agaggtagag gatgccgcgc
     6841 gcctgctggg tgaaatgcgt cgcgggtgtc agcaggcgga agaacagctc aaaatgctga
     6901 tcgattatca gaatgaatat cgcaataacc tcaacagcga tatgagtgcc gggatgacca
     6961 gcaaccgctg gatcaactat cagcagttta tccagacgct ggaaaaagcc attactcagc
     7021 atcgccagca acttaatcag tggacgcaga aagttgacat tgccctgaac agctggcgag
     7081 aaaagaaaca acgtttgcag gcctggcaga cactgcagga acggcaatcc acggcggcac
     7141 tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag cgcgccgcca
     7201 tgaggaaacc tgaatgattc gcttagcgcc cttaattacc gccgacgttg acaccaccac
     7261 attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcgcgttgt tgagcgaagc
     7321 attagcaggc gagacaacta ccgacaaagc ggccccccag ttgctggtgg caacagataa
     7381 gcccacgaca aaaggcgagc cgctggtcag cgagattctt gccgatgcgc aacaagcgga
     7441 tttactgatc cctgtggatg aaacaccgcc tgtcatcaac gacgaacaat ccacatcaac
     7501 accattaacc accgctcaaa cgatgacgtt ggctgcggtg gctggcaaca atacggcaaa
     7561 agacgaaaaa gcggatgatc tgaatgaaga cgtcaccgca agcctgagcg ccctttttgc
     7621 gatgttgccg ggttttgaca atacgcccaa agtgactgat gtgccgtcaa ccgtgttacc
     7681 ggcagagaaa ccaacgctct tcacaaaact gacttctgcg caactcacaa cagcacagcc
     7741 tgatgatgcc cccggcacgc cagctcagcc attaacaccg ctggtagcag aagcccagag
     7801 taaagcggaa gtcatcagca caccttcacc ggtgaccgct gccgccagcc cgctaatcac
     7861 tccacaccag acacagccac tgcccaccgt cgccgcgcct gtgttgagtg caccgctggg
     7921 ttctcacgaa tggcaacatt cattaagcca gcatatttcg ctgttcaccc gccaggggca
     7981 acaaagtgca gagttgcgtc tgcacccgca ggatttaggt gaagtgcaaa tctccctcaa
     8041 agtggatgat aaccaggcgc aaatccagat ggtttcaccg catcaacacg tacgcgccgc
     8101 cctagaagca gcgcttccgg tactgcgaac gcagctggcc gaaagtggca ttcagttagg
     8161 gcaaagcaac atcagtggcg aaagctttag tggtcagcag caggccgctt cccagcaaca
     8221 gcaaagccaa cgcacagcaa accatgaacc tctggcgggg gaagacgacg atacgcttcc
     8281 ggttcccgtc tctttacaag ggcgcgtaac aggcaacagc ggcgttgata ttttcgccta
     8341 acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct caagacgcag
     8401 gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac tgattacgcg
     8461 ataagcaaga aaagcaagcg atcgctttgg atcccgattc tggtattcat taccctcgcg
     8521 gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgccgc tgacgacaaa
     8581 gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt tacggtcaat
     8641 ctgggcgatg cggatcgcgt gctgtatatc ggcataaccc tgcgcctgaa agatgaagct
     8701 acacgctcgc ggctgagtga gtatttgccg gaagtccgta gccgtttgct gttactgttt
     8761 tcgcgtcagg atgctgccgt actggcgaca gaagaaggca agaaaaacct gattgccgag
     8821 attaaaacca cgctttccac cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac
     8881 gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc tttctcaagc
     8941 tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga cagccagtgt
     9001 tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg ttgtgcgcga
     9061 acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc gtatggggct
     9121 gttcaacctg ctgcgtcgta gcccggatat aaccgtcggg gccatccgca ttcagccgta
     9181 ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg aaccttatcc atctgaaacc
     9241 gctgcgcggc acagggctgg tggtgttttc accgagtctg gtgtttatcg ccgtggataa
     9301 cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt ttacccatac
     9361 cgaacagcgc gtcatcaacc gcatgttaaa gctggcgctt gaaggctata gcgacgcctg
     9421 gaaggcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg tgaaatttac
     9481 caatatcacc acctcgccga acgacattgt ggttaacacg ccgttccatg tggagattgg
     9541 caacctgacc ggcgaattta atatctgtct gccattcagc atgattgagc cgctacggga
     9601 attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa
     9661 cctggtgcgc caggtgcagc attcacagct ggagttggtc gccaactttg ccgatatctc
     9721 gctacgcctg tcgcagattt taaaactgaa acccggcgac gtcctgccga tagaaaaacc
     9781 cgatcgcatc atcgctcatg ttgacggcgt tccggtactg accagtcagt acggcaccct
     9841 caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa attctctgaa
     9901 cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa cggcgcaatg
     9961 gacgatctgt gggctgaagc tttgagcgaa caaaaatcaa ccagcagcaa aagcgctgcc
    10021 gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca ggatatcgac
    10081 ctgattatgg atattccggt caagctgacc gtcgagctgg gccgtacgcg gatgaccatc
    10141 aaagagctgt tgcgtctgac gcaagggtcc gtcgtggcgc tggacggtct ggcgggcgaa
    10201 ccactggata ttctgatcaa cggttattta attgcccagg gcgaagtggt ggtcgttgcc
    10261 gataaatatg gcgtgcggat caccgatatc attactccgt ctgagcgaat gcgccgcctg
    10321 agccgttagt gatgaataac cacgctactg tgcaatcttc cgcgccggtt tctgctgcgc
    10381 cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc gctgctgcct
    10441 ggctggtaaa acggttggga tttgccccta aacgcactgg cgttaacggt ctgaaaatta
    10501 gcgccagtgc ttcgctgggc gcgcgtgaac gggttgtggt ggtcgatgtg gaagatgcac
    10561 ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt cccccttcag
    10621 caccaacgga agagataccg cagcccgatt ttcagtcggt catgaaaaac ttgcttaagc
    10681 gtagcgggag atcctgatgc gtcgtttgtt gtctgtcgcc cctgtccttc tctggctgat
    10741 tacgcccctc gccttcgcgc aactgccggg tatcaccagc cagccgctgc ctggcggtgg
    10801 acaaagctgg tcgctcccgg tgcagacgct ggtgttcatc acctcgttga cgtttattcc
    10861 ggcaatttta ctgatgatga ccagtttcac ccgcatcatt attgtttttg gtttattgcg
    10921 taacgcgctg ggaacaccct ccgcgccacc taaccaggta ttgctggggt tggcgctgtt
    10981 tttgaccttt tttattatgt cgccggtgat cgacaaaatt tatgtcgatg cgtaccagcc
    11041 attcagcgaa gagaaaatat caatgcagga ggcgctggaa aaaggggcgc agccgctgcg
    11101 tgagtttatg ctgcgtcaga cccgtgaggc ggatttaggg ttgtttgcca gactggcgaa
    11161 tactggcccg ttgcagggac ctgaagccgt gccgatgcgc attttgctcc cggcctacgt
    11221 gaccagcgag ttgaaaaccg catttcagat aggcttcacg attttcatcc cttttttgat
    11281 tatcgacctg gtgatagcca gcgtgttgat ggcattaggg atgatgatgg ttccccccgc
    11341 caccattgct ctgcccttta aactgatgct gtttgtactg gtggatggct ggcaattgct
    11401 ggtcggttcg ctggcgcaga gcttttacag ctagagaggc aaaatgacac ctgaatcggt
    11461 catgatgatg gggactgaag cgatgaaagt cgcgctggca ctggctgccc cgctattgtt
    11521 ggtagcgttg gtcacgggcc ttatcatcag tattttgcag gccgccaccc agattaacga
    11581 aatgacgctg tcgtttattc cgaaaatcat cgccgtattt attgccatta ttattgccgg
    11641 accgtggatg ctcaatctgt tgctggatta cgtcagcacc ttgttcacta acctgccgta
    11701 tatcatcggg tagccgtact atgttgcagg tgacaagcga tcaatggctt tcctggctaa
    11761 gtctgtactt ctggccgtta ctgcgcgtgc tggcgctcat ttctaccgcg ccaattctga
    11821 gcgaacgcag cgtgccgaaa cgggtaaaac tgggtctggc aatgatgatc acgttcgcta
    11881 ttgccccatc attacctgcc aacgatgttc ctgttttttc gttctttgct ctgtggctgg
    11941 ccgtgcagca gatcctgatc ggcattgcgc ttggtttcac catgcaattt gcctttgccg
    12001 ctgtgcgaac cgctggcgaa attatcggtc tgcaaatggg gctgtcattt gcgacgtttg
    12061 tcgatccggc cagccatctc aatatgcctg ttttagcgcg tatcatggat atgctgacgt
    12121 tactgctgtt cctgacattt aacggtcatt tatggttgat ttcactgctg gtcgatacct
    12181 ttcacaccct gccaattggt ggcgaaccgt tgaacagcaa tgcgtttctg gcactcacca
    12241 aagcagggag tttgattttc cttaacgggc tgatgctggc gttaccgctc attactctgc
    12301 tgctgaccct gaatctggca ttaggtttac tgaatcgtat ggccccgcaa ttatccattt
    12361 ttgttattgg atttccatta actctgactg tcggtatctc tttaatggcg gcattaatgc
    12421 cgttaattgc acctttttgc gaacatttat tcagtgagat gtttaatttg ctggctgata
    12481 ttattagtga attgccatta atataattcc gtaacgttta tcatgttatc ctaaggatta
    12541 tccgaaaaat aatacctacg aacatcttcc aggatactcc tgcggcgaaa tatttgtttt
    12601 aagctcactc acatatcgca acatttactt tactttaaga caattccagg caaattatac
    12661 aacactttac gggatagtaa gtccgcctga aaaatcgcga gagtggcgca ttaggtgacc
    12721 catgttgttc cgtttagtca tgatgaaata ttcaggtaag gggaattatc gttacgcatt
    12781 gagtgagggt atgccatgtc aacgattatt atggatttat gtagttacac ccgactaggt
    12841 ttaaccgggt atctgttgag tagaggggtt aaaaaaagag aaatcaacga cattgaaacc
    12901 gttgatgacc ttgccatagc ttgtgattca cagcgccctt cagtggtgtt tattaatgag
    12961 gactgtttca tccatgatgc ttctaatagt cagcacatca agcacatcat taatcaacat
    13021 cccaatacgt tatttatcgt ttttatggca attgccaatg ttcattttga tgaatatcta
    13081 ttggtcagaa aaaatttatt gatcagttct aaatcgatta aaccggaatc tctcgacgat
    13141 atccttggcg atattctgaa aaaagagaca acgataacct cttttttaaa tatgccgacg
    13201 ttatcattga gccgaaccga atcgagtatg ttgcgaatgt ggatggcagg tcagggaacg
    13261 attcaaatct ctgaccaaat gaatatcaaa gccaagaccg tttcatcgca taaaggtaat
    13321 attaaacgta agatcaaaac gcataataaa caggttatct accatgtcgt ccgactgacg
    13381 gataatgtga ctaatggtat ttttgtcaac atgcgctaac acattctgac tggtggtttc
    13441 ccaccagtca ggctgaataa gattactctg ctttctccac aaagataccg tcctgatgcc
    13501 ctgcttcatt aaagaaccag atgccgagcg ggtagtcttc cagcgaaacc aggtacattg
    13561 tgccttcact aaactcctca actgccagta ccacgccagg acgacgcgga ccgccatccg
    13621 ttttgactgt tacccgatca ttcaccttca tcgttttcct cctgtggctt tgtgccagtg
    13681 tagaacaatt tcgtttttct ggcagcgcca ggcgcgcgcg agtgctgatt ttctcgacgg
    13741 tctatactta agagatgcca ggcggactta acgactggcg gcaacaacag agtaacggtt
    13801 gcgaggaaag atgatgaaaa ccgcaaaaga gtacagcgat accgcaaaac gtgaggtcag
    13861 cgtcgatgtc gatgccctgc tggcggcgat caatgaaatt agcgaaagcg aagttcatcg
    13921 cagccagaac gattctgaac acgttagcgt cgatggacgt gaatatcata catggcgtga
    13981 attggcggat gccttcgaac tggatattca tgacttcagc gtctctgaag tgaatcgttg
    14041 aatgcacaat aaaaaaatcc cgaccctgag ggggtcggga tgaaacttgc ttaagcaaga
    14101 agcacttaaa aaattcgtta caccaggaaa tctgatgtgt tcatcacctt atccgcaatt
    14161 tttttcgctg acaagaaaat attcgcgata tgaatgatta acgctactta tgaatagaaa
    14221 tgtgacgtgc gtcacccctt ggtggctctg gcgtcagcgg gatcgctccc gaaaaaatca
    14281 tctttgataa tttgtgctgt tttgagaatc atcctggtaa ggggagtact gcgcgactat
    14341 gttttcaatt caacaaccac tactggtttt tagcgatctt gatggcaccc tgctggacag
    14401 tcatagttat gactggcaac cagctgcccc ctggctcagc cgtttacgcg aagcgaatgt
    14461 tcccgtcatt ctctgtagca gtaaaacttc agcggaaatg ctgtacttgc aaaaaacgtt
    14521 ggggctacaa gggttaccgc tgattgccga aaatggcgca gtgatccagc ttgctgaaca
    14581 atggcaggat atagacggtt ttccacgcat catctcaggt attagccatg gcgaaatcag
    14641 ccaggtttta aatacgctac gcgagaaaga acattttaaa ttcacaactt ttgatgatgt
    14701 cgacgatgca accatcgccg aatggacggg attaagccgt agccaggcgg cgctgacgca
    14761 gctgcatgag gcgtcggtaa cgctaatctg gcgcgacagt gacgagcgta tggcacaatt
    14821 taccgctcgt ctgaacgaac tgggcttgca gtttatgcag ggtgcgcgct tctggcacgt
    14881 actggatgcc tctgccggaa aagatcaggc tgccaactgg attatcgcga cctatcaaca
    14941 acaattgtca ggcaaacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc
    15001 cttactggag gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtgca
    15061 tctgcatgat gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac cggaaggatg
    15121 gcgtgaaggg ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg cgcatacccg
    15181 attacgcccg gcctgtttag cgagataaag tcgacggtca gccagtgact ggagttgttc
    15241 aaaatcataa tcaccggttt cctcgctgct gcttaccccc agcgaggcac tgatgcgtat
    15301 cgtcgtactc ttggcgatca acatctcttt ttcatttaat ttcagtcgaa tacgttctgc
    15361 gacctccgca gcctccgtca gactcgcgcc tggcagaatc acacaaaact cctcaccacc
    15421 gacccgcccg gcaatgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga
    15481 aagaacacgg tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag
    15541 gtcgacctga atgacagaaa aaggatgttg gtgtgtctga cacaatttgg cgagcggacg
    15601 ggctttttcg aacagtgcgc cacgattata taaacgggtt aaggtgtcgt gccacgcctg
    15661 ccactgcaac gagctttgca gaacatacat gttgctgacc atccggcgaa tcacatacca
    15721 ggagatgagt aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact
    15781 gccgaaatcg ccgcgcacgc cttcgcttag cgtatggacg cgcaccagca caccgtcgaa
    15841 atggtccaga cgttcccagc taacatagcg actgttcata cgaatgccgc cccgtgtgtc
    15901 atgttccatt gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg
    15961 atgatcagga ttggaagagg tcaataatct cagcttactg tcatagagct gatactcacc
    16021 atcgaggttt ttatcgatgg cgtttcttaa aaattgctgc atggtacgca cgggaatact
    16081 catccccagc acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg
    16141 cggttcagta ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg
    16201 attttctcgt tgtgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt
    16261 cgggacattg cgcataaaca aggtcggctg cgtcgaaacg taaaatccgg cacgcgagac
    16321 atacatcgcc tgttcaacca tcgacgagga gttgtgtgcc agtcgcagca agtaaccaac
    16381 ttccagtgca gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggagattccc
    16441 ctcgctgact aatgcatccg atacgccatt gactgacagg gtgcgtcgtc ggttgagttc
    16501 aatttgccag gcgtgctcgt cgcgatgctg ttcgaactcg gttacagcat tacgcagtga
    16561 agtgaaatcc agtggcgcta cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt
    16621 gtcgacgtta tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg
    16681 ctggctggag atataggcat cttccagcac cactacttca cgccaggtga gcaaggtgga
    16741 aaaaagcaat accacgataa agcagagatt aacgacatga ccaggcccca ggcggcgtgc
    16801 gagttttttc aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc
    16861 caacctgctt ttttatgatt ctggcataac ctggcgatag cgtaccgcaa aacagcgata
    16921 attttataat ggtggcatga taagacgcga ctatcattca ttgcaggcat tggcacacca
    16981 ggccggatgt gatccggcct ggtgttaatg ataccctttc aaacttcttc cgcagaacga
    17041 ctcccctccc ccggaacaag ctcggtttca tgaaaagctt cccgcttgac cccgtaaccg
    17101 tcataccaac gacactcaac cataccgctg gagtatccgg tgacaatcat ccgcgggccg
    17161 ccctctttaa ccgtaacttc ctcactaacc ataaagctca tactcgcctc cttttttcga
    17221 gtgaaacttg ttcaccttag ttgaagatgg cgaattttgc ataatagcct gtgcgagtat
    17281 taatgtgcct gaccgcgtat tttcgctacg gctttcactc ccagcaccac gatgccgccg
    17341 atgataaatc caagaatcag atttaaaaca gtcggtaata tcatcgccac cactgcactt
    17401 tgctgcccgg cgaaatgttc aatggcgtga tgcagcggcg caattccatg taccacaatc
    17461 ccaccgccga cgaggaacat cgccagcgtg ccgacaatcg ataacgcctt catcaaccag
    17521 ggcgcaatga tcaataaccc tttacctaat gcctgcatca gcgcgctgga tttttccgcc
    17581 agccaatacc ccaggtcatc aatcttaacg ataaccccta ccagaccgta gacgcccacg
    17641 gtcactacca gcgcgatgcc tgaaagcacc agcacctgat taagcaacgg cgcttcggcc
    17701 acaatcccca gcgtgatggc gacgatttcg gcagacaata taaaatcggt acgaatcgcc
    17761 ccttttattt tgtccttttc aaacttcagc ggatcctgcg ccgccagctt ctccagacgc
    17821 tgctggctct gcgccggatc ttctttatgt ttacgcgcct ccagcatatg cagcactttc
    17881 tctactcctt caaagcagag aaacgcgcca ccaatcatca acagaggcgt aatcgcccac
    17941 gggataaacg cactgatgat cagcgccagc ggcaccagaa tcactttatt aatcagcgat
    18001 cctttcgcca cgccccagac cacgggaagt tcccggttgg cccgcacgcc tgaaacttgt
    18061 tgcgcattga gcgataagtc atcccctaat acaccggcgg ttttcttcgc cgccagtttg
    18121 cccatcacgg agatatcgtc cagtagtgtg gcgatatcat cgagcaacgt cagtaaacta
    18181 ctcccggcca aaagcattcc ctctcatttt tattgttgaa gggtgtaagt atgaagcaaa
    18241 aacggcagct ctgatacttg cttcacaggt caacattttt ttaacaatca agtgcaatta
    18301 tatctctcgc cagcagaatg attttcacgt ttactatgaa ccaccttttt atttccaccg
    18361 tgagggatta tgcgtttccg ccagttgtta ccgctttttg gcgcgctgtt tgcgttgtat
    18421 atcatttggg gctcaaccta ttttgtcatt cggattggcg tggaaagctg gcctccgtta
    18481 atgatggcag gcgttcgatt cctggcagcc ggtattttat tgatggcatt tttgctactg
    18541 cgcggacaca agctcccccc gctacgtccg ctgctcaatg ccgcgctgat tggcctgtta
    18601 ttgctggctg tcggtaatgg catggtgacg gttgccgagc atcaaaatgt tccttccggc
    18661 atcgccgccg ttgtggttgc aaccgtgccc ctctttaccc tgtgcttcag ccgcctgttt
    18721 ggcattaaaa cgcgcaaact ggaatgggtg ggtatcgcca ttgggcttgc cggaatcatc
    18781 atgctcaata gcggtggaaa tttaagcggc aatccgtggg gcgcgattct gattttaatc
    18841 ggctcgatta gctgggcgtt tggctcggtt tatggctcgc gcattacctt acctgtaggg
    18901 atgatggcgg gtgcgattga gatgctggcg gcaggcgtgg tgttaatgat cgcgtcgatg
    18961 attgcaggtg aaaaactgac ggcgctccct tccctttcag gcttccttgc ggtcggctat
    19021 ctggcgctgt ttggttcgat tatcgccatt aacgcttata tgtatttaat ccgtaatgtc
    19081 agtccggctc tcgccaccag ctacgcctac gttaacccgg tggtcgcggt cttgctgggt
    19141 acgggactgg gcggagaaac gctgtcgaag attgaatggc tggcgctcgg cgtaattgtc
    19201 ttcgcggtgg tgctggtcac gttgggaaaa tatctctttc cggcaaaacc cgtagttgcg
    19261 ccagtgattc aggacgcatc aagcgagtaa atgaatcccc tgcgtgtcga tctgcgcgct
    19321 ggcaccttcg ccgcagatcc actcttccag acgctcgcta agcgcttcat ccgtcagctt
    19381 ctcgcgccca cgtaacgcac actcccagac aatcagtacg cgccagccaa gctcctgcaa
    19441 gcgactgata tcacggcgat cgcgctcaac atttttacct atcttctcca gccagaactc
    19501 ggttcgagtc gcaggcactt taaacagata gcagtgatga tgatgccaga agcagccgtg
    19561 ggtaaatatc acgcaacgat attcatcaac gacaaaatcc gggcgtccgg gcagactggc
    19621 gtcctgaacg cgaaacgcca ggccctgccc ggttaacaga ctggcgaggc gcttctcaat
    19681 cgccgtatca cgcgtggcaa tcgcgcgcat atttttgctg cgagtggcct tatcgtgaac
    19741 gtcggccatg ttgtgcctct tgctgacgca acgccaccgc ctgtttgatt tttggctcaa
    19801 gcagttttgc caccgcggca aagaccggca cgaccaccga gttaccgaac tggcgatagg
    19861 cctgagtgtc cgaaaccgga atgcggaatt tcgcttctcc cggcgcttca aaacccatta
    19921 agcgcgcgca ttcacgaggc gttaaccgac gtggacgatg ttgctgattc tgcggatcgt
    19981 caaagtcttt ctcacctttg gccatatccc agccgcgatc gattaaaatt tccgcgccat
    20041 ctttgtaata acgcgcagac agcgtacgcg tgacgctttg cggattgttc ggataaacca
    20101 ttccataacc gaagccgtta ccgcgcgcct gatgtttttt cgcatatcga tagaggtact
    20161 tccacagcac cggcgtcagg atatatttcg cctcgaccat cgggtccagc agctgcgcca
    20221 gcgtcactcg ctgcgcaggg aaacattcgc tgatatcacg cagggtaaaa tcggctttca
    20281 gattcagatc gcgacgaaaa cccaccagca cgatgcgttc acggtgctgc ggcagaaaat
    20341 gtttgccgtc gatgattttc ggatcgtctg gcccgttatc ttctgcatca gccacgtcat
    20401 agcccagttc gtccagcgtc tgcatgatga tgcggaacgt tttaccctgg tcgtgacttt
    20461 tcaggttttt gacgttttcg agcacaaaca tcgccggacg acgcgcgtcg ataatgcgca
    20521 ccacatcaaa gaacagcgtg ccctgagtat cgcaggcaaa accgtgcgcc cgcccgagcg
    20581 agttcttttt cgatacgcca gccagcgaaa atggctgaca agggaaaccg gccagtaaaa
    20641 catcgtgttc aggaatgtgt tgacgaatat gttccgccgc cgcctcatca ctcacgcctt
    20701 ctttatggct gagggtgatg tcgcggatat cttcattaaa atgatgcgtc gccggatcgc
    20761 aataatggtt ggctttataa gtgcgtaccg catgtttgtt ccattcgctg gtaaacacgc
    20821 actgtccgcc aatcgattca aaaccgcgac ggatgccgcc aattccggcg aatagatcga
    20881 taaagcgaaa cgcataatgc ggatgatgtg ccggtggttt gggtaataac gtttgcagat
    20941 gggcgaactc tttctcactt aaacggtgcc atgccgagtc attcgccagc gcacgtttta
    21001 aaattgccgc gctccagtga ttctcaccta caccattaag ctgcgccacc agcgttttaa
    21061 catcataaat ttgcagcagt ttctccagca ttgcctgtgc ggcattcccg gtgctgtatg
    21121 aatcggttac tgatatattt tcctgcatag atttcaccgg ccatctgaga tggccgacag
    21181 gttaacataa ttacagccat ttagggccac gcgctcagcc agcggatgaa aacgcatcta
    21241 tcaccttgtg atcgacacct tcattctccc ccgccagttc ggcactgagc ttcgccataa
    21301 actcgactaa aaaatgcgcg ttgtgctgcg ccagctgctt gcctcttgcg gtttgcatgg
    21361 tttgcggtag cttcagcaac ttagtctgga aatgatccag cgcgtagcgt ttatcatcaa
    21421 gcgggcgatg ctgtgcgaac ggatcttcac catcaaacag cgccacgccc aatgcccctg
    21481 aaacggcaaa cacgcgcgcc aggccaatcg cccccagggc ttccagccga tccgcatcct
    21541 ggacgatttt agcttccgtt gttaaggggg cgatttgcgc gctgaaactg tgagcggcaa
    21601 tggcatgaca aacggcctcg attttctccg ccggaaattg ctcgaactct tcacggagca
    21661 ggcgacgcgt ctcttctgcc gccaggattg aagaacgctg cctttgcgga tgatttttcg
    21721 ccaggctcac aatatcgtga aaataacagg cggttaaaat caccagcata tcgacatcgt
    21781 catctgccgc cagcttctgt gccgtagccc agacgcggcg aaaatgacac acgtcatggg
    21841 ccgcgtcctg gtgctgatga tggtttttca accagttttc gaactgtgcc tgccagtgtt
    21901 gtaagtccat acgcgctccg ttgccaaaga gtcgccacct tagcagtttt tattcatagc
    21961 gtctgctacg tgcgggacga ggtcgggtgt accaggcaat gctgcctaac aatgccccga
    22021 ccagagccat aaataaaaat ccgaccagtg cccctaacca ctttcccgcg gtagagccta
    22081 aatcgctttc aaatcccgac acgggggcat cggggatccg gctcatcacc cagatgtaac
    22141 gcgccattgc ccagacgaaa tagccacacc atgtatagaa agcccacaaa gccagtttgc
    22201 cgccagggct gcgagagagt ctctcttcca tctttgtcga accgtattcc attttgagat
    22261 aacccggaaa attcagactt agtactatta tgtgataaaa gtcacatttt tccacattgg
    22321 atgagccagt caagactcac tcattttttg aacaaataga taatttttgc gcaacggcag
    22381 tgaatacagg aaaaatcgtt ttgagcgatt ttatttatct ttgagatcgg ctttattaat
    22441 tcattta
//
LOCUS       DAFNBJ010000077        22411 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000077 DAFNBJ010000000
VERSION     DAFNBJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.077"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..446
                     /locus_tag="L2X49_002756"
     CDS             <1..446
                     /locus_tag="L2X49_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBN4797823.1"
                     /translation="TVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSI
                     LDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCG
                     YTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
     gene            complement(486..1700)
                     /gene="ydcO"
                     /locus_tag="L2X49_002757"
     CDS             complement(486..1700)
                     /gene="ydcO"
                     /locus_tag="L2X49_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HBN4797824.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSGAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1753..2289
                     /gene="sutR"
                     /locus_tag="L2X49_002758"
     CDS             1753..2289
                     /gene="sutR"
                     /locus_tag="L2X49_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HBN4797825.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2362..4323
                     /gene="rlhA"
                     /locus_tag="L2X49_002759"
     CDS             2362..4323
                     /gene="rlhA"
                     /locus_tag="L2X49_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HBN4797826.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4415..4645)
                     /locus_tag="L2X49_002760"
     CDS             complement(4415..4645)
                     /locus_tag="L2X49_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HBN4797827.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4867..5021
                     /locus_tag="L2X49_002761"
                     /pseudo
     CDS             4867..5021
                     /locus_tag="L2X49_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            5067..5483
                     /gene="hicB"
                     /locus_tag="L2X49_002762"
     CDS             5067..5483
                     /gene="hicB"
                     /locus_tag="L2X49_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HBN4797828.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5562..6968
                     /locus_tag="L2X49_002763"
     CDS             5562..6968
                     /locus_tag="L2X49_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBN4797829.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7213..8358
                     /locus_tag="L2X49_002764"
     CDS             7213..8358
                     /locus_tag="L2X49_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN4797830.1"
                     /translation="MSKTFARSSLCALTMTIMNAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8376..9389
                     /locus_tag="L2X49_002765"
     CDS             8376..9389
                     /locus_tag="L2X49_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN4797831.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9390..10331
                     /locus_tag="L2X49_002766"
     CDS             9390..10331
                     /locus_tag="L2X49_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4797832.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10321..11115
                     /locus_tag="L2X49_002767"
     CDS             10321..11115
                     /locus_tag="L2X49_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4797833.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11137..12561
                     /gene="patD"
                     /locus_tag="L2X49_002768"
     CDS             11137..12561
                     /gene="patD"
                     /locus_tag="L2X49_002768"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBN4797834.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12658..12753)
                     /gene="yncL"
                     /locus_tag="L2X49_002769"
     CDS             complement(12658..12753)
                     /gene="yncL"
                     /locus_tag="L2X49_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBN4797835.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            12948..13121
                     /gene="ortT"
                     /locus_tag="L2X49_002770"
     CDS             12948..13121
                     /gene="ortT"
                     /locus_tag="L2X49_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBN4797836.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            13207..13440
                     /locus_tag="L2X49_002771"
     CDS             13207..13440
                     /locus_tag="L2X49_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBN4797837.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(13441..13890)
                     /locus_tag="L2X49_002772"
     CDS             complement(13441..13890)
                     /locus_tag="L2X49_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBN4797838.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(13887..14405)
                     /gene="mddA"
                     /locus_tag="L2X49_002773"
     CDS             complement(13887..14405)
                     /gene="mddA"
                     /locus_tag="L2X49_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBN4797839.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14586..15623
                     /gene="curA"
                     /locus_tag="L2X49_002774"
     CDS             14586..15623
                     /gene="curA"
                     /locus_tag="L2X49_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBN4797840.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPNYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            15821..16486
                     /gene="mcbR"
                     /locus_tag="L2X49_002775"
     CDS             15821..16486
                     /gene="mcbR"
                     /locus_tag="L2X49_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBN4797841.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16522..18624)
                     /locus_tag="L2X49_002776"
     CDS             complement(16522..18624)
                     /locus_tag="L2X49_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBN4797842.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNLGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            18866..19927
                     /locus_tag="L2X49_002777"
     CDS             18866..19927
                     /locus_tag="L2X49_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBN4797843.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(20042..21541)
                     /gene="ansP"
                     /locus_tag="L2X49_002778"
     CDS             complement(20042..21541)
                     /gene="ansP"
                     /locus_tag="L2X49_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HBN4797844.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEILFKPETAS"
     gene            21808..>22411
                     /locus_tag="L2X49_002779"
     CDS             21808..>22411
                     /locus_tag="L2X49_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBN4797845.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNTLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKR"
BASE COUNT         5681 a         5523 c         5798 g         5409 t
ORIGIN      
        1 cgaccgtcag caaaaaccac gcaatgatag tcattgagga tttgaaggtc agcaacatgt
       61 caaagtcagc agcgggtacg gtcagccagc cggggcgcaa tgtccgggca aaatcaggtt
      121 taaaccgttc gatactggat cagggctggt atgaaatgcg ccgccagctt gagtacaagc
      181 agctctggcg tggcggtcag gtgcttgctg ttccgccagc gtatacaagc cagcgttgcg
      241 cgtactgtgg tcatacagcg aaagagaacc gcctgtcaca aagtaaattc agatgccagg
      301 tatgtggata tacagcgaac gccgatgtaa atggcgctcg taacatttta gcggcggggc
      361 acgccgttct tgcctgtgga gagatggtgc agtcaggccg ctcgttgaag caggaaccca
      421 ccgaaatgat tcaggcgaca gcctgaacgt agcagggagc cacgtccttc agggcgtgga
      481 ggatgtcaat atcggtttct gtcagcgatt aaattcaaca ccacgtaaca aacgcctccg
      541 gcaattaatc cccagaacgc agaaccaatc ccgaccagcg tcaatccact tgccgttacc
      601 agaaatgcca ccaccgccgc gtctcgctca cgctcattat gcagcgcctg atacaaactg
      661 ccgctaatgg tacttaacag cgccagacct gccagcatct ggatccaact tacgggcagg
      721 gcagccatca tcccggtaat agcactacca aacagacctg cgatcaaata aaaaatgcct
      781 gcaacggcag ctgccagcca acgttgatct ttatccggat gcgcttccgg gctttggcaa
      841 atagccgcgg tgattgccgc aataccgacg gaataaacgc cgaaagggga aaaaaccagt
      901 gccagcaatc cagtaaatac aattaatggc gaaacaggag ccgaataccc ggctgctttc
      961 attgctgcga tacccggtgc gttttgcgat gccatcgtca ccagaaaaag ggggagtgca
     1021 acgctcaggc tgtgagcaaa cgaaaaatca ggggtaatat aagtggggag aacgggttta
     1081 aagacaacat cagttgtgac aacgtcacct tgcgcgataa cgatcacgat cccaataatc
     1141 atcgcggcaa ttaccgcata gcgcggcgca acggccttgg ttgccagcca taccagcaac
     1201 atacttccac acaacgtaaa ttgaccgtcc agactggcaa acgcctgtaa accaaagcgt
     1261 aataaaatcc cggcaagcat tgccgccgca agcgagtgcg gaataatgcg catcagacga
     1321 gcaaagagtc ccgttatgcc gcagaggact attaacgcgt tggtgacaat aaaaacgccg
     1381 atggcttcgt taagtgttag tccctgcaat ccggtgacca acaaagccgc gccaggcgtt
     1441 gaccatgcgg tgagaacagg tacgcgatac cataatgtca gagtcagcgt actgacgccc
     1501 attgccagcc ccagcgccgt catccagcca gagatttgtg cagtggtggc tccggcgaca
     1561 atcgctgctt gccagattat tgcccctgaa ctggcgtagc caattaatac cgccagaaac
     1621 cccgccagta gcgtgggtgg aggaatagaa aacagacgca tagtcacctc gtacgttata
     1681 gcgtacaggc accgtagcat ttgtccgtta taacgcacaa gtgataaact tccgttttgc
     1741 cggaggagtc gcatggaaaa tctcgctcgc tttttatcca ccacacttaa acaactacgc
     1801 cagcagcgcg gctggagtct ttcgcgactg gcagaagcga cgggcgtttc taaagcaatg
     1861 ttagggcaga ttgagcgtaa tgagtccagc ccgacggtcg cgacattatg gaaaattgcc
     1921 accgggttga atgtgccatt ttcgacattt atttctccgc cacagtccgc tacgccttca
     1981 gtttatgatc cacaacagca ggcgatggtg atcacttcgc tgtttcctta tgatccacaa
     2041 ctctgctttg aacacttctc gattcagatg gcacccggtg caataagcga atcgacgccc
     2101 catgagaagg gggtaatcga acatgtcgtc gtcattgatg gacaacttga tctgtgcgtt
     2161 gatggggaat ggcagaccct taattgtggg gaaggcgttc gatttgctgc agacgtcacg
     2221 catatctatc gtaatggtgg ggagcaaacc gtacattttc attccctcat ccattacccg
     2281 cgcagttaag cgggaaaact atttcgcaac gtcgcgcttg tggctaaaat agccgccatt
     2341 tttcagctac tggataagaa tgtgaccgta tcttctcatc gacttgaact gttaagcccg
     2401 gcacgcgatg ccgccattgc ccgcgaagct attttgcacg gtgccgatgc tgtttatatc
     2461 ggcggccctg gttttggtgc ccgtcataat gccagtaata gcctgaaaga tattgccgag
     2521 ctggtgccgt ttgcccatcg ttatggtgca aaaattttcg tcacgcttaa caccattttg
     2581 catgatgatg agctggaacc cgcgcaacgg ctgattactg acctctacca gaccggtgtc
     2641 gatgcgctga ttgttcagga tatggggatt ctggaacttg atattccgcc gattgaactg
     2701 cacgccagta cgcagtgcga cattcgtaca gttgaaaaag cgaagttcct ctctgatgtt
     2761 ggcttcacgc agattgtgct ggcgcgagag ctgaatcttg atcagatccg cgcgattcac
     2821 caggctacgg acgcgaccat tgaattcttt attcatgggg cactgtgcgt ggcctattcg
     2881 ggtcagtgct acatttctca tgcgcaaaca gggcgtagcg ccaaccgtgg cgattgctcg
     2941 caggcgtgcc gtttgccata cacattgaaa gacgatcagg ggcgggtggt ttcctatgaa
     3001 aaacatctgc tgtcgatgaa agataacgat cagactgcca acctcggcgc gctgattgat
     3061 gctggtgtac gctccttcaa gattgaaggg cgttacaaag atatgagcta cgtgaagaat
     3121 atcaccgccc attatcgcca gatgcttgat gccattattg aagaacgtgg cgatctggcg
     3181 cgcgcttcat caggtcgtac tgaacatttc tttgttccat cgacggaaaa gactttccac
     3241 cgtggtagca cagattattt tgtgaatgcc cgtaaaggcg atattggcgc gttcgattcg
     3301 ccgaaattta tcggcctgcc ggtaggcgaa gtattgaaag tggcgaaaga tcatctcgat
     3361 gttgccgtta ccgagccact ggcaaatggc gatggcctga acgtgttgat taaacgtgaa
     3421 gtcgtcggtt ttcgtgccaa tacggtcgag aaaaccggag aaaatcagta ccgcgtctgg
     3481 cccaatgaaa tgccagcaga tttgcacaaa attcgtccac atcacccact aaaccgtaat
     3541 cttgatcata actggcagca ggcactgaca aaaacctcca gcgaacgtcg ggtggcggta
     3601 gacattgaac tgggcggctg gcaggaacaa ctgattctga ccctcaccag tgaagagggt
     3661 gtcagcatca cgcatacgct ggacgggcag ttcgacgaag ccaataacgc cgaaaaagca
     3721 atgaacaatc tgaaggatgg tctggcaaaa ctggggcaaa ccctctatta cgcccgcgat
     3781 gtgcaaatta atttgccggg ggcgctgttt gtaccaaaca gtctgttaaa ccagttccgc
     3841 cgtgaagctg ctgacatgct ggatgctgcg cgtcttgcca gttaccagcg cggcagccgt
     3901 aaaccggttg ctgatcctgc gccggtttat ccgcaaacgc atctgagttt cctcgcgaac
     3961 gtatacaacc agaaagcgcg tgaattttat catcgctatg gtgtgcagct gattgacgcg
     4021 gcgtatgaag cacatgaaga gaagggcgaa gtcccggtga tgatcaccaa gcattgtctg
     4081 cgctttgcct ttaatctgtg cccgaaacag gcgaaaggca atatcaaaag ctggaaggcg
     4141 acgccaatgc aactggttaa cggcgatgaa gtattaacgc taaagtttga ttgccgccca
     4201 tgcgagatgc acgtcattgg caaaatcaaa aatcacatac tgaaaatgcc gttaccggga
     4261 agcgtagtgg catccgtaag tccggatgag ctgctgaaaa cattgcctaa gcgaaaaggg
     4321 taaaacgcca gttttctggt tactcacaac tcattgaatc tgcatgatat tgcctgccgg
     4381 gtaaggcgtc acgccgcatc cggcaacaaa tgactcaacg ttgtcccgct tctggtttgc
     4441 gcgatttttg ccagtaaaaa tgctcgcgca aaccttccgc cgactcttcc gccacagcac
     4501 gcaattcatc gctgtccgct tcatgacgca gctgatgatc cacattcttt acccacacaa
     4561 attcatgtcc tttgtgccct gccatgagtt gtcctgagaa cagcgcacac gttaataaga
     4621 caaccgataa cgccttcgta aacatcctgc cacctttttc ttaccttttg ccgctatgat
     4681 gccgatcgtt tcttgaggtt attattcagt tttgcaaatt agcgcaaaga aattctggaa
     4741 tcttccttcc tgattttgca ttgcattctg ccgttgcggc gatttagtgc tattttcgag
     4801 caaattacac acagaggtaa acactaatgt ttactttgtt gatatactca gcggcaggga
     4861 ggcgatgtga aacaaagcga gttcagacgt tggctcgaat ctcagggcgt cgatgtagcg
     4921 aatggccgca accatttgaa actcaggttt catgggaggc gcagtgtcat gccgcgtcac
     4981 tcctgcgtaa agcaatcctg aaacaactcg gtttgagtta atcgccaatt aaaaaggtta
     5041 atgacatgcg agagacagtc gaaattatgc gttatcccgt cactcttaca cccgcgccgg
     5101 aaggcggtta tatggtttct tttgtggata tccctgaagc gttgacgcag ggcgaaactg
     5161 tcgctgaagc gatggaagcg gcaaaagatg ctttactgac cgcatttgat ttttattttg
     5221 aagataacga gcttatcccg ttaccttcgc cattaaatag tcatgatcac tttattgaag
     5281 tacctttgag cgtcgcctct aaagtattgc tgttaaatgc ttttttacag tcagaaatca
     5341 ctcagcaaga gttagccagg cgaattggca aacctaaaca ggagattact cgcctattta
     5401 acttgcacca tgcgacaaaa atcgacgccg tccagcttgc ggcaaaggcg cttggcaaag
     5461 agttatcgct ggtgatggtt taattacagt taacgaaaag ttgtcatttt taacaactga
     5521 tatagactgc cgaatcatct gcacataatt acgattcgat aatgaaaaaa taccagcagc
     5581 ttgcagaaca attacgcgag cagattgcgt cgggtatctg gcaacccggc gatcgtttgc
     5641 cttcgttgcg tgaccaggtg gcgctttcag gcatgagctt tatgactgtc agccatgcct
     5701 atcagttgct cgaaagtcag ggatatatta tcgcacgacc gcagtcgggt tattacgttg
     5761 cgccacaggc aataaaaatg ccgaaagcgc cagtcattcc agtcactcga gatgaagcag
     5821 tcgatatcaa cacttatatt tttgatatgt tgcaggccag tcgcgatccg tcggtcgttc
     5881 cgtttgcctc ggcctttccc gacccgcgac ttttccccct ccaacaacta aaccgctcgc
     5941 tggcgcaggt aagcaaaacc gccacagcga tgagcgtgat tgaaaactta ccgccaggaa
     6001 acgcagaact gcgtcaggct attgctcgtc gctatgcctt acagggcatc accatttctc
     6061 ctgatgaaat tgtcattact gccggggcgt tagaggcatt aaacctcagt ttgcaagcgg
     6121 taactgaacc gggcgattgg gtgatagtag agaatccttg tttctacggt gcgttgcagg
     6181 cgctggagcg gctacggctg aaggcgttat cggtggcgac ggatgttaaa gaagggatcg
     6241 atcttcaggc gctggaactg gcgttgcagg attatccggt gaaagcgtgc tggctgatga
     6301 ctaatagcca gaacccactc ggatttacct taacgccgca aaaaaaagca caactggtgg
     6361 cgttactcaa tcagtacaac gtaacgctga ttgaagatga cgtttacagc gaactttatt
     6421 ttggacggga aaaaccgctg cctgcgaaag cgtgggatcg ccacgatggc gttttgcatt
     6481 gctcttcgtt ttcgaaatgt ctggtgcctg gttttcgtat tggttgggtc gccgccggaa
     6541 aacatgcacg taaaattcaa cgcttgcagt tgatgagtac gctttccacc agctcaccga
     6601 tgcaacttgc gctggtggat tacctttcca cgcgccgata cgacgcccat cttcgtcgcc
     6661 tgcgtcgcca gcttgcggaa cgtaaacaac gtgcctggca ggcactgctg cgttatctgc
     6721 ctgcggaagt taaaattcat catagtgaca gcggttattt tctctggctg gagctccccg
     6781 agccgttaga tgccggcgaa ttaagcctgg cggcactgac gcatcatatc agtattgcgc
     6841 cgggtaaaat gttttctacc ggtgaaaact ggtcacgttt ttttcgtttt aataccgcgt
     6901 ggcagtgggg agaacgtgaa gaacaggcgg taaaacaatt aggcaaactt attcaagaac
     6961 ggctgtaata gcgtttaatt taattcctct tagattgggt aatatgaatt tcgaatagca
     7021 gtcatatttc ctaacccctt gtctatactc cagaagataa ccttacagac ggcataatgc
     7081 gcggtagctc acaacctgat taaattttct caggggcgaa ggtgtgcctg caagccgccg
     7141 tctatggtta aacaaggaga tatttttacg gcacggcggc tgaacaatta attacgacag
     7201 gagtaagacc ttatgagcaa gacatttgcc cgcagcagcc tgtgtgcgct caccatgaca
     7261 ataatgaacg ctcacgccgc cgaaccacct accaatttag ataaaccgga agggcgactg
     7321 gatattatcg cctggccggg atacatcgaa cgcggacaaa ctgataaaca atacgactgg
     7381 gtaacgcaat ttgaaaaaga gacaggctgc gcggtgaatg tgaaaaccgc cgcgacttcc
     7441 gatgaaatgg tcagtctgat gaccaaaggg ggttacgatc tggttacggc atccggcgat
     7501 gcctcgctgc gtttgattat gggcaaacgc gtgcagccga ttaataccgc cttgattccc
     7561 aactggaaaa cgctcgatcc gcgcgtggtt aaaggcgact ggtttaatgt tggcggcaaa
     7621 gtttacggca caccttacca atgggggccg aacctgctga tgtacaacac taaaaccttc
     7681 ccgacgccgc cggatagctg gcaagtggtt tttgttgagc aaaatctgcc ggacggcaag
     7741 agcaataaag gccgcgttca ggcttatgat ggccctatct acattgcgga cgctgcgttg
     7801 ttcgttaaag ccactcagcc gcagttgggc atcagcgatc cgtatcaact aaccgaagaa
     7861 cagtaccagg cggtgctgaa agtgctgcgc gctcaacaca gtttgatcca tcgctactgg
     7921 catgacacta ccgtgcaaat gagcgatttc aaaaacgagg gtgtggttgc ttccagtgcc
     7981 tggccctatc aggccaacgc cctgaaagcc gaaggccagc ctgttgctac cgttttcccg
     8041 aaggagggtg ttaccggttg ggctgatacc accatgctgc atagcgaagc gaaacatccg
     8101 gtttgcgcct acaaatggat gaactggtca ttaacgccaa aagtgcaggg cgatgtggcg
     8161 gcctggtttg gctcgttacc ggtagtgccg gaagggtgta aagccagtcc gttattaggc
     8221 gaaaaaggtt gtgaaaccaa cggttttaac tatttcgaca aaatcgcctt ctggaaaacg
     8281 cctatagcag aagggggcaa gtttgttccc tacagtcgct ggacgcagga ttacattgcc
     8341 attatgggcg gtcgctaact tcgctggggt gctttatgac gtacgcagtg gagtttgaca
     8401 acgtctcgcg gttgtacggt gacgtgcgcg cagtagatgg cgtcagtatt gcgataaaag
     8461 atggtgagtt cttctctatg ctggggccgt ccggctccgg caaaaccacc tgcctgcgcc
     8521 tgattgctgg cttcgaacag ctttccggcg gggctatctc tatctttggt aaacccgcca
     8581 gcaatctgcc accgtgggag cgggacgtga atactgtctt tcaggactac gcgctatttc
     8641 cgcatatgtc gattcttgac aatgtcgcct atgggctgat ggtcaaaggc gtgaataaaa
     8701 agcagcggca cgcaatggcg caagaggcgc tggagaaagt ggcgttgggg tttgtacatc
     8761 aacgtaaacc gtcacaactt tctggtggtc agcgccagcg ggttgctatc gccagagcat
     8821 tggtgaatga accgcgcgta ttgctgttgg atgaaccgct cggcgcactg gatctcaaat
     8881 tgcgtgagca gatgcagctg gaactgaaaa aactgcaaca gtctctcggt atcactttta
     8941 tcttcgttac ccacgatcag ggcgaagcgt tatcgatgtc cgatcgtgtg gcggttttca
     9001 ataatggacg cattgagcag gtcgattccc cgcgcgatct ctatatgcgc ccgcgcacgc
     9061 cgtttgttgc cggattcgtc gggacatcca acgtttttga tggactgatg gcagagaaac
     9121 tttgtggcat gacgggaagc ttcgccctgc gaccggaaca tatccgcctc aacacccctg
     9181 gtgaactgca ggccaatggc acgatccagg cggtgcaata tcagggcgcg gcaactcgtt
     9241 ttgaactgaa attgaacggc ggtgaaaaac tgcttgtgag tcaggccaat atgacaggcg
     9301 aagaactgcc tgccacgctc acgcccggac aacaggtgat ggtttcctgg tcgcgtgatg
     9361 tgatggtgcc gctggttgag gagaggtgaa tggcgatgaa tgtattgcaa tcaccttcac
     9421 gtccaggtct gggtaaggtg tccggtttct tctggcgtaa tccggggctg gggctgtttt
     9481 tactgctgct tggcccgcta atgtggtttg gcattgtcta tttcggctcg ctgctgacac
     9541 tgttatggca gggattttat acttttgacg atttcaccat gtcggtaacg ccggaactga
     9601 cgctggcgaa tatccgtgcg ctgtttaatc cggcgaatta cgacatcatt ctccgcacgc
     9661 tgactatggc tgtggcagtc actatcgcca gcgccattct ggcttttcca atggcgtggt
     9721 atatggcgcg ctataccagc gggaaaatga aagctttttt ttatattgcg gtaatgttgc
     9781 cgatgtgggc gagctacatt gttaaagcct atgcctggac gttattgctg gcaaaagatg
     9841 gcgtggctca gtggttttta caacatcttg ggctggaacc actgctgact gcgttcctta
     9901 cattacctgc ggtgggcgga aatacgctgt caacttccgg gctggggcgc tttctggtgt
     9961 ttctctatat ctggttgccg ttcatgatcc tgcccgttca ggcggcgctt gagcgtttgc
    10021 cgccgtcatt gttgcaggcg tcggctgatc tcggcgcacg tccacgacaa acctttcgct
    10081 atgtggtgct gccgctggca atcccgggta ttgccgctgg ctctatcttt accttctcac
    10141 tcacactggg cgattttatc gtcccgcagc tggttggtcc tccaggatat tttatcggca
    10201 atatggttta ttcccagcag ggggcgattg gcaatatgcc gatggcggcg gcattcaccc
    10261 tggtgccgat tgttctcatc gcactgtacc tggcgttcgt gaaacgtctg ggagcgttcg
    10321 atgcactctg aacgcgcacc gtttttcctc aaactggcgg cctggggcgg cgtggttttc
    10381 ctacattttc ccatcctgat tatcgccgct tatgcgttta acactgaaga tgcggcgttt
    10441 agttttccac cgcagggcct gacgctgcgc tggtttagcg tggcagcaca gcgtagtgat
    10501 attcttgatg ccgtgacact gtcacttaaa gtggcggcgc tggcgacatt aatggcgctg
    10561 gtgttaggga cgctggcagc tgccgcgctg tggcgacgag actttttcgg caaaaacgcc
    10621 atttcgctgt tactgctgct gcccattgcg ctgccgggca ttgtcactgg tctggcgcta
    10681 ttaaccgctt ttaaaaccat caatctggag ccgggatttt tcaccatcgt ggtcggtcat
    10741 gcgacttttt gcgtagtggt ggtgtttaac aatgtcatcg cccgttttcg ccgcacctcg
    10801 tggagtctgg ttgaggcgtc aatggatctt ggggccaatg gctggcaaac cttccgctac
    10861 gtagtgttgc cgaatctcag ttcggcgcta ctggcaggag gaatgctggc gtttgccttg
    10921 tcgttcgatg aaatcatcgt tacgactttt acggcaggtc atgaacgaac gttaccgttg
    10981 tggttgctca atcagcttgg gcgaccgcgt gatgtaccgg taactaacgt ggtggcactg
    11041 ctggttatgt tggtaacaac cttgccgatc ctgggggcct ggtggctaac ccgcgaaggc
    11101 gacaatggtc aataaccact gatacaggaa tatgctatgc aacataagtt actgattaac
    11161 ggagaactgg ttagcggcga aggggaaaaa cagcctgtct ataatccggc aacgggggac
    11221 gttttactgg agattgccga ggcatccgca gagcaggtcg atgctgctgt gcgcgcggca
    11281 gatgcagcat ttgccgaatg ggggcaaacc acgccgaaag tgcgtgcgga atgtctgctg
    11341 aaactggctg atgttatcga agaaaatggt caggtttttg ccgaactgga gtcccgtaat
    11401 tgtggcaaac cgctgcatag tgcgttcaat gatgaaatcc cggcgattgt cgatgttttt
    11461 cgctttttcg cgggtgcggc gcgctgtctg aatggtctgg cggcaggtga atatcttgaa
    11521 ggtcatactt cgatgatccg tcgcgatccg ttgggggtcg tggcttctat cgcaccgtgg
    11581 aattatccgc tgatgatggc agcgtggaaa ctggctccgg cgctggcggc agggaactgc
    11641 gtagtgctta aaccatcaga aattaccccg ctgaccgcgt tgaagttggc agagctggcg
    11701 aaagatatct tcccggcagg cgtgattaac gtactgtttg gcagaggcaa aacggtgggt
    11761 gatccgctga ccggtcatcc caaagtgcgg atggtgtcgc tgacgggctc tatcgccacc
    11821 ggcgagcaca tcatcagcca taccgcgtcg tccattaagc gtactcatat ggaacttggt
    11881 ggcaaagcgc cagtgattgt ttttgatgat gcggatattg aagcagtggt cgaaggtgta
    11941 cgtacatttg gctattacaa tgctggacag gattgtactg cggcttgtcg gatctacgcg
    12001 caaaaaggca tttacgatac gctggtggaa aaactgggtg ctgcggtggc aacgttaaaa
    12061 tctggtgcgc cggatgacga gtctacggag cttggacctt taagctcgct ggcgcatctc
    12121 gaacgcgtca gcaaggcagt agaagaggcg aaagcgacag ggcacatcaa agtgatcact
    12181 ggcggtgaaa agcgcaaggg taatggctat tactatgcgc cgacgctgct ggctggcgca
    12241 ttacaggacg atgccatcgt gcaaaaagag gtatttggtc cagtagtgag tgttacgctc
    12301 ttcgacaacg aagaacaggt ggtgaactgg gcgaatgaca gccagtacgg acttgcatct
    12361 tcggtatgga cgaaagatgt gggcagggcg catcgcgtca gcgcacggct gcaatatggt
    12421 tgtacctggg tcaataccca tttcatgctg gtaagtgaaa tgccgcacgg tggacagaaa
    12481 ctttctggtt acggcaagga tatgtcactt tatgggctgg aggattacac cgtcgtccgc
    12541 cacgtcatgg ttaaacatta aggataatat tgcagatcgt aagagtatga gatgatcttg
    12601 cgtactgtca gagcactgat ttctctgaca gtacgtgctg cgtgcaaaat cactacatca
    12661 aataaaccag ccaaatctca tagagataag agtaaacaaa ccaacagcag caaagacatt
    12721 tatcagtact acagttctac tggaaacatt catttttgcc accttttatc attgccccta
    12781 tgtgtatagt acgggttaag aaaatccgca ataatactca ttctgaagat gaataagggc
    12841 aagttgctgt ttgatgattt tttctcaaac tggtgaaaaa acttgatgca cgtcaaaaaa
    12901 tgacgcatat ttgcgcgcgt tttattcatc tggctggacg cccgtacatg tctctctatc
    12961 aacacatgct tgttttttat gcggttatgg cagcaatcgc atttcttatc acctggtttc
    13021 tttctcacga taagaaacgc atccgtttct taagcgcctt tctggtgggg gcaacatggc
    13081 cgatgagttt cccggtggcg ctgttgtttt cactgtttta aatcttactc actggcttga
    13141 cggcaccacg cagacttata tcatttggat gaatcgataa atttcacaag tggctaagga
    13201 gaaagtatgt cgcatctgga tgaagtcatc gcgcgcgtgg atgccgcgat tgaagagagc
    13261 gtcattgccc atatgaacga attattaatc gccctgagcg atgacgcgga gttaagtcgg
    13321 gaagatcgct acacccagca gcagcgtttg cgcaccgcta ttgctcatca cggtcgcaag
    13381 cataaagaag atatggaagc gcgccacgaa cagttaacca aaggcggcac catcctctga
    13441 ttaaaatgag cgtctggcta ccaaccaggc gccaaccaca agtaaaattg cgccacaaat
    13501 tgggccgaac aacgcccgta gtggtacgcc atgactgcgg aagatatcca gcatcagacc
    13561 gccaattagc tggctggcga ccagtactgc aatagttgtc gccgctccca cattctgata
    13621 tccgctgata ctggcgaaga caaaaaacga tcccagcaag ccgggtataa gtgtccacca
    13681 gcgcacgctg gacaccagtt cgccaaaccc cgccatgcct tgtttaaacc ataaaataga
    13741 aacaaagagc acaatcccca ccagtgagtt cagcaacatg gcgatgagaa tggtagagga
    13801 ggtctgggtg atgcgcacca tcagcgtgtt ctggactacc agaccaattc cggcggcaat
    13861 caaaaaagca agggtgagcg actgattcat ccgatcgcgt ccggttcagt gcgttcgtcg
    13921 agttgcaact gcataaatgt cagatccagc cagcgcccaa atttagtgcc cacctgcggc
    13981 atttgtgcgg tgacgacaaa tcccagcgaa tggtgcagat gcagcgaggc ctgattttgc
    14041 gattcgatcc cggcgaccat gacatgcttc ccgcaatccc gcgcttcatc aatcaatcgg
    14101 cttaacaatt tacgccccag acctttgccc tgatgatcgg gatggacata aaccgaatgt
    14161 tccacggtat ggcgaaaacc atcgaaacta cgccagtcgc caaacgaggc atatcccgtc
    14221 actacgccat cttcttcact taccagcacc ggataacctg ctatggtccg cgcttcaaac
    14281 caggcaatgc ggttatctgc atcaaccgtt tggtcattcc agatagccgc cgtatacaac
    14341 acggcgtggt tataaatttc cgcaatggca gcacaatcgg ctttgcgggc aaaacggatg
    14401 gacatgtctg gcctcgataa aataatgatg atgatgttat caatactatc gaaaacagcc
    14461 cgaagcaacg gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca
    14521 aacaaaacca atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga
    14581 gcattatggg gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg
    14641 gcgcaccggt tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag
    14701 gacaggtgtt actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga
    14761 gcgatgagcc atcttattca ccgcctgttg atattggcgg cgtgatggtc ggcggcacgg
    14821 tgagccgtgt tgtggagtcg aatcatccta attatcagcc tggcgactgg gtgctgggct
    14881 acagtggatg gcaggactat gacatatcca gtggtgatga tctggtgaaa cttggcgatc
    14941 atccgcaaaa tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata
    15001 tggggctgct ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga
    15061 caggtcctgt gggagcaacg gtggggcaaa tcggcaaact taaaggttgc agggtggtgg
    15121 gggttgccgg tggcgcggaa aaatgccgcc atgctatcga ggtgctgggc ttcgatgttt
    15181 gtcttgatca ccacgcggat gattttgccg aacaactggt gaaagcgtgc ccaaaaggta
    15241 ttgatatcta ttatgaaaac gtgggcggca aggtatttga tgcagtgcta ccattactta
    15301 atacatctgc gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac
    15361 cacccggtcc ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct
    15421 tgcaagggtt tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga
    15481 tggggcaatg ggtgaaagag gataaaatcc actaccacga agatattact gatggtttag
    15541 agaatgcgcc acagacgttt atcggcttgc tgaagggtaa aaacttcggc aaagtggtga
    15601 tccgcgtcgc gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag
    15661 gcaaaaagct tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac
    15721 aacaaaggtg ggtggtttat gattattcac tttaatacac caggtgaact ccttctgcca
    15781 tgcaggcagg gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat
    15841 gaaacatgtc agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg
    15901 cgcattaaaa cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag
    15961 tattacacct gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc
    16021 acctgcacaa gcatttacag ttccggaagt ggggaaacgt caattagacg aaatcaatcg
    16081 gatccgctac gaactggaat taatggcagt tgctctggct gttgaaaacc tcaccccgca
    16141 agaccttgcg gaactccagg aattgctcga gaaattacag caggcgcaag aaaagggcga
    16201 catggaacaa atcattaatg taaacaggct atttcgctta gcgatttatc atcgctcaaa
    16261 tatgcccatc ctgtgtgaga tgattgagca actgtgggtc aggatggggc ctggtttaca
    16321 ttatctctat gaagcgatta atccagcgga attacgggag catatagaaa actatcatct
    16381 gttactcgct gcgttaaaag caaaagacaa agagggatgc agacattgtc ttgctgaaat
    16441 tatgcaacaa aatattgcta ttttatacca gcaatacaat cgttaaagta aatgcccggc
    16501 aaataccggg catcaaggta attactcaaa tctccacgca atattcatgc cgacaccata
    16561 atttcgtccg ggcgaaggtt cgtaatatcg cccgtttgac tcattgacaa tgacagaacc
    16621 aacgtattct ttatcgaata aattatcgac acgaccaaat aaatccacag ttaaattgtg
    16681 gtaattgtat ttatacccgg tgaataagcc gacgagagta taagacggcg cttttgccgt
    16741 attttcatca tctgccataa tatcgcccat ataacgcgct tccgtgcctg cataccaacc
    16801 atcttccggt acataaccta tcgacgcaaa gcccatatta cgggcgatcc ctggcatccg
    16861 attaccgtta caatcctgtt cattgcaaac attgctgcga taggtcgcat caagccaggt
    16921 ccaggacgcg tttacgcgaa aatctcctgc gaaacgttga tcccatgcca gttcagcgcc
    16981 ttgacgacgg gtctttccgg catttttgta agtcgtacgc ccaccgctac tgctatcgac
    17041 aacaatttca tcatcagtgt cggtctgaaa caatgcgaga ctaagcagcc catcaccaat
    17101 acgcgtttta ctgccgatct caattgtatc gttggtggat ggttttaaac ctaagttcat
    17161 accgctttgc ccatcagcac gataagacag ctcattaatc gtcggcgttt caaaacctcg
    17221 cccggctgcc agatagatat tccaggcatc ggtcattgca tattttaacg aaccggcagg
    17281 tagccattta tgataactgg catcaccgct gtcatcgccg ttacccggag taacgtaatg
    17341 gtcgttggaa tcaaaccaca cggagctgta gcgcacgcca gcatccagcg acagtttttc
    17401 gctcagctgc cactgcgtct gtaaataggg atcgatgttc cacatcagat tgcgttcgtc
    17461 gcgacgcaac tcaccttttt gcccgtactc cggcatgccg ctattcaggc ggaagttatt
    17521 gtagcccttg cggttttcac tcatgttttc gtagttcagg ccggtagtga acgtgaccgg
    17581 aacgcccagt tcaccacggt gtgtccagcg gctgtctatc ccctggtaat ggcgttgcag
    17641 ggtaatcacg ccgcccgcat gtgacgggtt aagttggggg gccatcggga ttgactggta
    17701 ctgggtcgtt tctcgctctc cggcatacat catcacactc atatcatctt gcgcgctcag
    17761 gctacgctca tagcgcaacc cagcctgagt ttgcttgatg gtttttcgcg tgtcgtactg
    17821 ttctgcacga ggcgcttgtt gtggattcgc cttccattct gctttggtta gcccacctgg
    17881 gtcatctgct ttgatatcca cactattgaa aatcaggctt aatttgctgg cttcatcaat
    17941 gcgtacgccc agtttggcat tggctaaatt tttctgtgcg ccactatggt cacgatagcc
    18001 gtgggtcgta aaacgcgtgg ttgagacggt gtaatcgaca tcgccgggct gtgtaccgtc
    18061 tcccgttgcg cccgttgctt tcagcccata gcgccagctg ccaaaactgc cgtagtaact
    18121 actggcttca atggttggtg gctgttgtcc ggtctgggtg gtgacattca ttacaccgcc
    18181 agacgcgttg ccatacaggg cagagaaggg gccacgcagc acttccacat tttgcacact
    18241 gcttaaatcg atgttggatg tttgcccttg cccgtcgggc atggtggcgg gaataccgtc
    18301 cacatacagg cgaataccgc gaattccgta agtggaacgg gagccaaatc cgcgaatcga
    18361 cagctgtaaa tcttgcgcat agttctgccg gttttgtacc tgcaaaccag gcacgctggt
    18421 cagtgattcg gacaagttaa tgcgcggtgt tgccaggcgc atctcctcgc catccaccac
    18481 gcttactgct gctggcgtat ccagctcgga aaccacctgc ggtgcggcac tgacaatcat
    18541 agtctgttca tcagcggcaa aaacaacggg ggaaaggaca agcagtgcgg gcaaaacggt
    18601 ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta agaatgggaa aacgctgtca
    18661 tggtaataaa attgtaaatt tatggaaaac gaaacggcac gatacgttaa ttaattgaga
    18721 aaattgtagt cgtaacggca agaaatgctc cacatttgag aaaataatga ttaccattcc
    18781 catttataac aagagcgtaa cgatgattac gcttagcgaa gcattgtgaa gcagcaaaaa
    18841 tattggttca tcaaagggag tcgtcatgca tttacgtcat ctgttttcat cgcgcctgcg
    18901 tggttcatta ctgttaggtt cattgcttgt tgcttcatca ttcagtacgc aggccgcaga
    18961 agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa atggcttata gccagcaaga
    19021 aaacgcgctg tggctcgcca cttcgcaaag ccgcaaactg gataaaggtg gcgtggttta
    19081 tcgtcttgat ccggtcactc tggaagtgac gcaggcgatc cataacgatc tcaagccgtt
    19141 tggtgccacc atcaataaca cgactcagac gttgtggttt ggtaacaccg taaacagcgc
    19201 ggtcacggcg atagatgcca aaacgggcga agtgaaaggc cgtctggtgc tggatgatcg
    19261 taagcgcacg gaagaggtgc gcccgctgca accgcgtgag ctggtagctg atgatgccac
    19321 gaacaccgtt tacatcagtg gtattggtaa agagagcgtg atttgggtcg ttgatggcga
    19381 gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa atgagtaccg gtctggcgct
    19441 ggatagcaaa ggcaaacgtc tttacaccac taacgctgac ggcgaattga ttaccatcga
    19501 caccgccgac aataaaatcc tcagccgtaa aaagctgctg gatgacggca aagagcactt
    19561 ctttatcaac attagccttg ataccgccag gcagcgtgca tttatcaccg attctaaagc
    19621 ggcagaagtg ttagtggtcg atacccgtaa tggcaatatt ctggcgaagg ttgcggcacc
    19681 ggaatcactg gctgtgctgt ttaacccagc gcgtaatgaa gcctacgtaa cgcatcgtca
    19741 ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa gtggtgaaaa cgttcgatac
    19801 gccgactcat ccgaacagcc tggcgctgtc tgccgatggc aaaacgctgt atgtcagtgt
    19861 aaaacaaaaa tccactaaac agcaggaagc tacccagccg gacgatgtga ttcgtattgc
    19921 gctgtaatag atatggcgag ggtgaaaacg gagagacttg cctgactttt atcacgccgc
    19981 atgcggcaca ccttcgtcag taatttgaaa ttgcccggca cgtagccggg caattttgca
    20041 attagctcgc cgtttcaggc ttaaacaaga tttcctgttt ttcttcatct tcctcgacga
    20101 ctggcgcagt gctgtgaatt tcagcaacgc gtttgcgcac accaaaccag cctataacca
    20161 gcagaatacc gataatcggc agcgccgcga tagtataagt cccgttcggg taatcgaacg
    20221 ccatcagcac aaggacactc agtaaaaaca gtaatgtcag ccaggaagtg aagggcgcgc
    20281 caggcagttt aaaactgaca tccgctgctt tgccttcttt aatcgcttta cgcaggcgca
    20341 tctggcacac gatgataaac gcccatgaag cgatgattcc cagcgacgcg aagttcaaca
    20401 caatctcaaa tacgcgcgac ggcaccagat agttgaggaa tacgccgacg acatacacaa
    20461 ctagtgttgc tagaatcccg gcatacggca catgctgacg actcattttc gccataaaac
    20521 tcggtgcgga accgcccatc gccattgagc gcagaatacg tccggtgcag tacagacctg
    20581 agttcaggct ggagagggca gcggtcagca ccacaatgtt cataatgctg ccgatatatg
    20641 gcacacccag tttagagaaa aacgtcacga acggactttg ccccgcctga tacgcgctcc
    20701 acggcaatag cataaccagc aacaccacgg agccgacgta aaacaggcca atacgccaaa
    20761 tcacgctgtt aatcgcttta ggcaccatgg tctgcggatc tttacattct ccggcagctg
    20821 tacccaccat ttcaatggag gcaaaagcaa acactacgcc ctgaatcaac accagcgcag
    20881 gcagcaaacc gtgggggaag aagccgccat tatcggtgat taaatgaaag ccagtggtgt
    20941 tgccatccag cggctgacca ctaccgagga acactgtacc cacgaccaaa aaggtcacaa
    21001 tggcgagcac tttaataagc gcaaaccaga actccatctc cgcaaaccat ttcacaccga
    21061 tcatattcat ggtgccaacg atggtaagtg cagcgagcgc aaagacccac tgcggcacgc
    21121 cgccaaacgc accccagtaa tgcatataca gagcgacggc ggtaatatca acaatccccg
    21181 tcatcgccca gttgatgaag tacatccagc cagcaacata agcggctttc tcacccaaaa
    21241 actcacgggc ataagagaca aaactgccac tggaagggcg gtgtagcacc agctcaccca
    21301 atgcacgcag aataaaaaac gaaaacaagc cacaaattaa ataaaccagt gccagtgctg
    21361 gacccgccat ttgcagtcgg gctcctgcac ctaaaaacaa gccggtgcca atcgcgccgc
    21421 caatggcgat catctgtacc tggcgattgc ccatcgcttt gtgatacccc tcttcgtggg
    21481 cattaagcca gcggcgtttc gcggcgtgtt gatctgaagt gtcggtgtcg tgtttactca
    21541 ttgctctccc tgattgcttt aatgaaaaag tcatataagt tgccatgaac aatgtttatt
    21601 catcctggcg atgatagtta tcaccattat tctttatggg tataaaacta tcggcgcagc
    21661 atcctacccg ttatcgataa acgatgcaaa acatcccctt ataatcctga aggggattaa
    21721 tacaactgac gaaaaaatga caaatccttt tgctggttaa cctgtgtact gtcctacact
    21781 taatctttaa aagattgtga ggggcatatg attaaggtgt atggcgtacc cggatggggc
    21841 tctacaatca gtgagctgat gctaacactg gctgatattc cttatcagtt cgttgatgtc
    21901 agtggttttg accacgaggg aacttcacgc gagttattga aaaccctgaa cacgttgtgt
    21961 cagattccga cactggcgct ggaaaatgac gagattatga cggagacagc ggcgattgca
    22021 ttgatggttc tcgatcgtcg cccggacctt gcgccaccgg ttgggcgcgc cgaacgtcag
    22081 cagtttcaac ggctattggt ctggctggtt gctaatgtct atccaacatt cactttcgcc
    22141 gattaccctg agcgttgggc tcctgacgca cctgaacagt taaagaagaa tgttattgaa
    22201 tatcgtaaat cgctttatat ctggctgaat tcacaattga ccgctgaacc ttatgcgttt
    22261 ggtgagcaac taacgctggt ggactgttat ctttgcacta tgcgcacatg ggggcctggg
    22321 catgaatggt ttcaggataa tgccacgaat atcagtgcga ttgctgatgc tgtatgtcag
    22381 ttaccgaaat tacaagaagt attaaaaagg a
//
LOCUS       DAFNBJ010000078        22393 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000078 DAFNBJ010000000
VERSION     DAFNBJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.078"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..1318)
                     /locus_tag="L2X49_002780"
     CDS             complement(<1..1318)
                     /locus_tag="L2X49_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBN4797846.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYAQSWREAEPDNEDAPYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLLVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQACQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQ"
     gene            complement(1315..2022)
                     /locus_tag="L2X49_002781"
     CDS             complement(1315..2022)
                     /locus_tag="L2X49_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBN4797847.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2186..3163
                     /locus_tag="L2X49_002782"
     CDS             2186..3163
                     /locus_tag="L2X49_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBN4797848.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIREIEDLL"
     gene            complement(3233..3715)
                     /locus_tag="L2X49_002783"
     CDS             complement(3233..3715)
                     /locus_tag="L2X49_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBN4797849.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            3950..6532
                     /gene="leuS"
                     /locus_tag="L2X49_002784"
     CDS             3950..6532
                     /gene="leuS"
                     /locus_tag="L2X49_002784"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBN4797850.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6547..7128
                     /gene="lptE"
                     /locus_tag="L2X49_002785"
     CDS             6547..7128
                     /gene="lptE"
                     /locus_tag="L2X49_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBN4797851.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7128..8159
                     /gene="holA"
                     /locus_tag="L2X49_002786"
     CDS             7128..8159
                     /gene="holA"
                     /locus_tag="L2X49_002786"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBN4797852.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8161..8802
                     /gene="nadD"
                     /locus_tag="L2X49_002787"
     CDS             8161..8802
                     /gene="nadD"
                     /locus_tag="L2X49_002787"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBN4797853.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            8826..9437
                     /locus_tag="L2X49_002788"
     CDS             8826..9437
                     /locus_tag="L2X49_002788"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBN4797854.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            9697..10014
                     /gene="rsfS"
                     /locus_tag="L2X49_002789"
                     /gene_synonym="rsfA"
     CDS             9697..10014
                     /gene="rsfS"
                     /locus_tag="L2X49_002789"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBN4797855.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10018..10485
                     /gene="rlmH"
                     /locus_tag="L2X49_002790"
     CDS             10018..10485
                     /gene="rlmH"
                     /locus_tag="L2X49_002790"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBN4797856.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10516..12417
                     /gene="mrdA"
                     /locus_tag="L2X49_002791"
     CDS             10516..12417
                     /gene="mrdA"
                     /locus_tag="L2X49_002791"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBN4797857.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12420..13532
                     /gene="mrdB"
                     /locus_tag="L2X49_002792"
                     /gene_synonym="rodA"
     CDS             12420..13532
                     /gene="mrdB"
                     /locus_tag="L2X49_002792"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBN4797858.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13543..14631
                     /gene="rlpA"
                     /locus_tag="L2X49_002793"
     CDS             13543..14631
                     /gene="rlpA"
                     /locus_tag="L2X49_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBN4797859.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14771..15982
                     /gene="dacA"
                     /locus_tag="L2X49_002794"
     CDS             14771..15982
                     /gene="dacA"
                     /locus_tag="L2X49_002794"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBN4797860.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16093..16356
                     /locus_tag="L2X49_002795"
     CDS             16093..16356
                     /locus_tag="L2X49_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBN4797861.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16457..17098
                     /gene="lipB"
                     /locus_tag="L2X49_002796"
     CDS             16457..17098
                     /gene="lipB"
                     /locus_tag="L2X49_002796"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBN4797862.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17357..18310
                     /locus_tag="L2X49_002797"
     CDS             17357..18310
                     /locus_tag="L2X49_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBN4797863.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQQTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            18519..19484
                     /gene="lipA"
                     /locus_tag="L2X49_002798"
     CDS             18519..19484
                     /gene="lipA"
                     /locus_tag="L2X49_002798"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBN4797864.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(19585..19788)
                     /gene="tatE"
                     /locus_tag="L2X49_002799"
     CDS             complement(19585..19788)
                     /gene="tatE"
                     /locus_tag="L2X49_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBN4797865.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(19917..20705)
                     /locus_tag="L2X49_002800"
     CDS             complement(19917..20705)
                     /locus_tag="L2X49_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBN4797866.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            20798..21181
                     /gene="crcB"
                     /locus_tag="L2X49_002801"
     CDS             20798..21181
                     /gene="crcB"
                     /locus_tag="L2X49_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBN4797867.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21235..21444)
                     /gene="cspE"
                     /locus_tag="L2X49_002802"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21235..21444)
                     /gene="cspE"
                     /locus_tag="L2X49_002802"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBN4797868.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(21619..22179)
                     /gene="pagP"
                     /locus_tag="L2X49_002803"
     CDS             complement(21619..22179)
                     /gene="pagP"
                     /locus_tag="L2X49_002803"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBN4797869.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5709 a         5619 c         5572 g         5493 t
ORIGIN      
        1 gctgcggttg cggctccggt tcaacagttt gcttctcggt aaaattcgcc gcctgaatta
       61 ataatcgacg aataattaat gcttccagtg gacaacgatt ttctgcatca aggcggctca
      121 caatcggcat taatgattct tctgtaggtt ggcaggcctg cactaattgt ttggcaacta
      181 gatataatgg ccataaggga tgttctgtat cacgcgtgag tgagatccgc ggcttacgtc
      241 ctttgtgcaa aaccatctcg accagtgaac gccccagcgc agagagtttt tcatcgctga
      301 gtatttcgta taaatgaaaa cgcgtacggg cgctgtcgcc ggggacataa agtagcggtt
      361 ttccctgatc gttaaccaga tcacgcgctt caatcatcat caccagtaat gggtaataat
      421 cgacccgatg ctgacggcac cattgcaaca ttaaagcatc cgcctgggtg gagggatact
      481 cgcgccagta gtcacaaagt tctgccaaca ggctttcgcc ttctccacaa tagacgcgtt
      541 gcgcgtattc ataataaggc gcatcttcat tatcaggttc agcttcccgc catgactggg
      601 cgtaatccag aagttcgggg attcccaagc ggcttgcgct ataccagcgg agtaaatttc
      661 tgtgcagttt tgcatcatcg gggatgatca ctgcgcgatg ttgcgctaaa tacaatgtca
      721 gccattctgg atgcgatgac caaatccgag cgaacatttg gtaaaaatcg atggtgtcat
      781 tttgcacggc aacgggcaga tgcagaatgg tctggaagtt aaaaacattc cccttcgcaa
      841 tggcatataa aaaggattcc agttcttctt cgtctatttc cccgacgttt tcctcctgca
      901 accaccgcaa tctttctgct aataacacca cgcactcgaa ggataaatgg gcagtgttca
      961 tggcgattgt gcacagcgac cagcgtaatt catcaatctc ttccatcgag caataattta
     1021 attgctgaat gaatcgctgc caggcggagg gcagaaaacg ttcgctatca gaggcaagta
     1081 acgcgcgaaa ggcgagcaga atttcatgtt ctgctacctc atattcttct ggttgccaaa
     1141 cagatttagc aggcgactgc gcaatccgga gcgcctcctc atacgcctga cgaagttgtt
     1201 taaaaccctg cggatcggtt tctggatgaa aggacggtaa cagcgccaga taagcgcggc
     1261 ggataatatc gacgtcagtc gtttcctcta tatcgaggat cttccagcaa tttttcatag
     1321 gtcgctcata tccttaagcg tttccgggca tgcagaatag taagccaatg gttgccagga
     1381 caaggtaggg taatacgcat catcactggc aaataattga taaaacatat cattgccgga
     1441 atcgtcgtat tctttacgcg ctaagaattc gcgtaataaa ggcagatgat catcttcggg
     1501 agactgccaa tattgccgac caaaggtata accggcaaag tattgccgcc agttactata
     1561 ataacgtaga gcccgtagat gaatgcgcga ctggatccat agactttctt cttcgctcaa
     1621 ccagttatta agtacgccca ttcggcttaa aaaacccatc cggacataat cccaggcttt
     1681 gattccccct tcgccacagc attcggcggt gctggcgaca aattgcgcat aagcctgacc
     1741 ttgctcattt agttcagcaa gatagtcacg ccattcacat ggcgaataac gaaaccagcg
     1801 gtgataaaac cctgccaacc gagcggcatg accgttatcg atcattcggt gaattgtgcg
     1861 cagcaggtca tcacggtcat caattcccca tgaggaagaa aggtcaaaaa agcatgtacc
     1921 taaatacatt ttgggtgatg aatgcgaagg taaacagcat tcattgcgta tttccatcgg
     1981 agtggaaagt gcaaacaata ttttttgcga ctccatgtcc atataattta tccttaaata
     2041 ttcattaaat gaatattagc cgcattgcga ggctcgttta tttatacttt tctgacgcga
     2101 cataatacgc ataaaataag gcttacgcaa aaatggaaca cataaaaata taatatgttt
     2161 tatctgcaac cataaggaaa tgataatgat tttcacgtca agttgctgcg ataatttatc
     2221 aatagatgag attatcgaac gtgctgaaaa aggcgattgc gaggctcaat atattgttgg
     2281 gttttattat aatcgcgata gcgcaattga ttctccggac gacgaaaaag ccttttactg
     2341 gctgaagctg gccgccgagc aaggtcattg tgaagcacaa tattccttag gacaaaagta
     2401 tactgaggat aaaagccgtc ataaagataa tgagcatgca atcttttggc tgaaaaaagc
     2461 tgccctacaa ggccatactt tcgcttccaa cgcccttggc tggatactgg atcgtggaga
     2521 agaccccaat tataaagaag cggttgtctg gtatcagata gccgcggaga gcggaatgtc
     2581 ttatgcgcaa aataatcttg ggtggatgta cagaaatggc aacggagtcg caaaagacta
     2641 tgcgctggca tttttctggt acaaacaagc tgcattacaa ggccatagtg acgcgcaaaa
     2701 caatctggcc gatctttatg aagacggaaa aggcgttgct caaaacgaga cactcgccgc
     2761 attctggtat ttgaaaagcg cacagcaggg taatcggcat gctcagtttc aaattgcatg
     2821 ggattataac gctggcgaag gggtggacca ggactataag caagcgatgt actggtatct
     2881 gaaggctgcc gctcagggga gcgtcggcgc ttacgtcaac atcggttata tgtataaaca
     2941 cggacaaggc gttgagaaag attatcaggc tgcctttgaa tggtttacga aagccgctga
     3001 atgcaatgac gccactgcct ggtataacct ggccattatg tatcactacg gagaaggaag
     3061 acctgtcgat ctccgacagg ctctcgacct gtatcgtaaa gttcagtcat ccggaaccag
     3121 agatgtcagt caagaaattc gtgagattga agatttactg tagagataaa aacaagggcc
     3181 ggggaatttc attcggccca tattctcttt attgctgtgc gaaatcagac tattacggct
     3241 caaacgggcg tctctggaac tggtcatgac cacatttcgg gcatagcgtc agcacttccg
     3301 gtgtgtagat cgggagatgg aagtgacatt tctcgcagac cagatttccc agcccgacca
     3361 cttctccgct gtgataaacc ccatgatgat tgaggtcctg gaaaacttcg cgccattcaa
     3421 gctgcgtttt atcggtgatg tctgccagct cctgccacaa gctttcttta atcacccgca
     3481 taaagacgct gtcagattct tctttcaggc tctcttcata gctcatggcg aactcttcca
     3541 ggtcacgtct gacagctcgc gtcagctcat cgacctcggt tcgcgttaac tcccctgttt
     3601 ttattacgcg ctcgcgcgcc tgttccacca gtgcgtcgat atcacgttcg ccattgcgca
     3661 ggcgttcgct cagtgacgcg accagttcac ggtaatattg agcaaccttg ttcatcgttt
     3721 catctcccgg gtcgttaact gactataata atagacgttc tttgttcctt gctttgctaa
     3781 tacgcccaca aactctgtaa attaatgtat gaataatccg ggcatcattt caacgtcgag
     3841 tgcgcgaaag gctgttttga cgcggacgtt tgggctatgc tatgcggatc tgaaaaacca
     3901 cctcaacgct acatttgtag ccgtattgaa aacaggacca ctggctgcca tgcaagagca
     3961 ataccgcccg gaagagatag aatccaaagt acagcttcat tgggatgaga agcgcacatt
     4021 tgaagtaacc gaagacgaga gcaaagagaa gtattactgc ctgtctatgc ttccctatcc
     4081 ttctggtcga ctacacatgg gccacgtacg taactacacc atcggtgacg tgatcgcccg
     4141 ctaccagcgt atgctgggca aaaacgtcct gcagccgatc ggctgggacg cgtttggtct
     4201 gcctgcggaa ggcgcggcgg tgaaaaacaa caccgctccg gcaccgtgga cgtacgacaa
     4261 catcgcgtat atgaaaaacc agctcaaaat gctgggcttt ggttatgact ggagccgcga
     4321 gctggcaacc tgtacgccgg aatactaccg ttgggaacag aaattcttca ccgagctgta
     4381 taaaaaaggc ctggtatata agaagacttc tgcggtcaac tggtgcccga acgaccagac
     4441 cgtactggcg aacgaacaag ttatcgacgg ctgctgctgg cgctgcgata ccaaagttga
     4501 acgtaaagag atcccgcagt ggtttatcaa aatcactgct tacgctgacg agctgctcaa
     4561 cgatctggat aaactggatc actggccaga caccgttaaa accatgcagc gtaactggat
     4621 cggtcgttcc gaaggcgtgg agatcacctt caacgttaac gactatgaca acacgctgac
     4681 cgtttacact acccgcccgg acacctttat gggttgtacc tacctggcgg tagctgcggg
     4741 tcatccgctg gcgcagaaag cggcggaaaa taatcctgaa ctggcggcct ttattgacga
     4801 atgccgtaac accaaagttg ccgaagctga aatggcgacg atggagaaaa aaggcgtcga
     4861 tactggcttt aaagctgttc acccattaac gggcgaagaa attcccgttt gggcagcaaa
     4921 cttcgtattg atggagtacg gcacgggcgc agttatggcg gttccgggtc acgaccagcg
     4981 cgactacgag tttgcctcta aatacggcct gaacatcaaa ccagttatcc tggctgctga
     5041 cggctctgag ccagatcttt ctcagcaagc cctgactgaa aaaggcgtgc tgttcaactc
     5101 tggcgaattc aacggtcttg accatgaagc ggccttcaac gccatcgccg ataaactgac
     5161 tgcgatgggc gttggcgagc gtaaagtgaa ctaccgcctg cgcgactggg gtgtttctcg
     5221 tcagcgttac tggggcgcgc cgattccgat ggtgacgctg gaagacggta ccgtaatgcc
     5281 gaccccggac gaccagctgc cggtgatcct gccggaagat gtggtgatgg acggcattac
     5341 cagcccgatt aaagcagatc cggagtgggc aaaaactacc gttaacggta tgccagcgct
     5401 gcgtgaaacc gacactttcg acacctttat ggagtcctcc tggtactatg cgcgctacac
     5461 ttgcccagag tacaaagaag gtatgctgga ttccgaagcg gctaactact ggctgccggt
     5521 ggatatctac attggtggta tcgaacacgc cattatgcac ctgctctact tccgcttctt
     5581 ccacaaactg atgcgtgatg caggcatggt gaactctgac gaaccagcga aacagttgct
     5641 gtgtcagggt atggtgctgg cagatgcctt ctactatgtt ggcgaaaacg gcgaacgtaa
     5701 ctgggtttcc ccggttgatg ctatcgttga acgtgacgag aaaggccgta tcgtgaaagc
     5761 gaaagatgcg gcaggccatg aactggttta taccggcatg agcaaaatgt ccaagtcgaa
     5821 gaacaacggt atcgacccgc aggtgatggt tgaacgttac ggcgcggaca ccgttcgtct
     5881 gtttatgatg tttgcttctc cggctgatat gactctcgaa tggcaggaat ccggtgtgga
     5941 aggggctaac cgcttcctga aacgtgtctg gaaactggtt tacgagcaca cagcaaaagg
     6001 tgatgttgcg gcactgaacg ttgatgcgct gactgaagat cagaaagcgc tgcgtcgcga
     6061 tgtgcataaa acgatcgcta aagtgaccga tgatatcggc cgtcgtcaga ccttcaacac
     6121 cgcaattgcg gcgattatgg agctgatgaa caaactggcg aaagcaccaa ccgatggcga
     6181 gcaggatcgc gctctgatgc aggaagcgct gctggccgtt gtccgtatgc ttaacccgtt
     6241 caccccgcac atctgcttca cgctgtggca ggaactgaaa ggcgaaggcg atatcgacaa
     6301 cgcgccgtgg ccggttgctg acgaaaaagc gatggtggaa gactccacgc tggtcgtggt
     6361 gcaggttaac ggtaaagtcc gtgccaaaat caccgttccg gtggacgcaa cggaagaaca
     6421 ggttcgcgaa cgtgctggcc aggaacatct ggtagcaaaa tatcttgatg gcgttaccgt
     6481 gcgtaaagtg atttacgtac caggtaaact cctcaatctg gtcgttggct aagcgcggga
     6541 ggaagcgtgc gatatctggc aacattgttg ttatctctgg cggtgttaat caccgccggg
     6601 tgtggctggc atctgcgtga taccacgcag gttccttcca ctatgaaggt catgatcctg
     6661 gactcaggcg atccgaacgg gccattaagc cgtgcggtgc gtaaccagtt acgtctgaat
     6721 ggtgtcgagt tgcttgataa agaaaccacg cgtaaggacg ttccatccct gcgtttgggt
     6781 aaagtgagca tcgcgaaaga taccgcatcg gtattccgta acggtcaaac agcagagtat
     6841 cagatgatca tgacggtgaa tgcgaccgtg ttgatccccg gccgtgatat ctacccgatt
     6901 agcgccaaag tcttccgttc gttctttgat aacccgcaaa tggcgttagc gaaagataac
     6961 gaacaagaca tgatcgtaaa agagatgtac gaccgtgctg ccgaacagct gattcgtaag
     7021 ctgccaagca tccgtgctgc ggatattcgt tccgacgaag aacagacgtc gaccacaacg
     7081 gatactccgg caacgcctgc acgcgtctcc accacgctgg gtaactgatg attcggttgt
     7141 acccggaaca actccgcgcg cagctcaatg aagggctgcg cgcggcgtat cttttacttg
     7201 gtaacgatcc tctgttattg caggaaagcc aggacgctgt tcgtcaggta gctgcggcac
     7261 aaggattcga agaacaccac actttttcca ttgatcccaa cactgactgg aatgcgatct
     7321 tttcgttatg ccaggctatg agtctgtttg ccagtcgaca aacgctattg ctgttgttac
     7381 cagaaaacgg accgaatgcg gcgatcaatg aacaacttct cacactcacc ggacttctgc
     7441 atgacgacct gctgttgatc gtccgcggta ataaattaag caaagcgcaa gaaaatgccg
     7501 cctggtttac tgcgcttgcg aatcgcagcg tgcaggtgac ctgtcagaca ccggagcagg
     7561 ctcagcttcc ccgctgggtt gctgcgcgcg caaaacagct caacttagaa ctggatgacg
     7621 ctgcaaatca ggtgctctgc tactgttatg aaggtaacct gctggcgctg gctcaggcac
     7681 tggagcgttt atcgctgctc tggccagacg gcaaattgac attaccgcgc gttgaacagg
     7741 cggtgaatga tgccgcgcat ttcacccctt ttcattgggt tgatgctttg ttgatgggaa
     7801 aaagtaagcg cgcgttgcat attcttcagc aactgcgtct ggaaggcagc gagccggtta
     7861 ttttgttgcg cacattacaa cgtgaactgt tgttactggt taacctgaaa cgccagtctg
     7921 cccatacgcc actgcgtgcg ttgtttgata agcatcgggt atggcagaac cgccggggca
     7981 tgatgggcga ggcgttaaat cgcttaagtc agccgcagtt acgtcaggcc gtgcaactcc
     8041 tgacacgaac ggaactcacc ctcaaacaag attacggtca gtcagtgtgg gcagagctgg
     8101 aagggttatc tcttctgttg tgccataaac ccctggcgga cgtatttatc gacggttgat
     8161 atgaaatctt tacaggctct gtttggcggc acctttgatc cggtgcacta tggtcatctt
     8221 aaacccgtgg aaacgctggc gaatttgatt ggtctgacgc gggtcacaat catccctaat
     8281 aatgttcctc cgcatcgtcc ccagccggaa gcgaacagcg tgcagcgtaa acacatgctt
     8341 gaactggcga ttgccgacaa gccattattt actcttgatg aacgcgagct aaagcgcaat
     8401 gccccctctt acactgcgca aacactgaaa gagtggcggc aggaacaagg gccggacgtg
     8461 ccgctggcgt ttattattgg tcaggattca ttgctgacct ttccgacctg gtacgaatac
     8521 gaaacgatac tcgacaatgc acatttgatc gtctgtcggc gtccaggtta cccacttgaa
     8581 atggcgcaac cgcaatacca gcaatggctg gaagatcatt tgacacataa cccggaagat
     8641 cttcaccttc agcctgccgg taaaatttat ctggctgaaa cgccgtggtt taacatctcg
     8701 gcgaccatca tccgcgaacg tttgcaaaac ggtgaatcgt gtgaggattt attgccggaa
     8761 ccggtactga cttacattaa ccaacaaggc ttgtatcgct gatacctgtc gttacggagg
     8821 gcatgatgcg actgtggtta attcgtcatg gcgaaacgca agcgaatgtc gatggtctgt
     8881 acagcggtca tgcgcccaca cccctgaccg cacgcggtat tgagcaagcg caaaatctgc
     8941 atacgctgct acatggtgtt tcctttgatc tggttttatg cagtgaactg gaacgggcac
     9001 agcataccgc gcgactggtt ctcagtgacc gccagctccc cgtgcaaatc atccctgaac
     9061 tcaacgaaat gttttttggc gactgggaga tgcgacatca tcgcgacctc atgcaagaag
     9121 atgccgaaaa ctatagcgcg tggtgcaatg actggcagca tgcaatcccc acgaacggtg
     9181 aaggatttca ggcattttcg caacgtgtgg aacgctttat cgcaaggctt agtgaatttc
     9241 agcactatca gaatatttta gtcgtcagcc atcagggtgt actgagtctg ttaatcgccc
     9301 gtttaattgg catgcctgcc gaggctatgt ggcattttcg cgttgaccag gggtgttgga
     9361 gcaccattga tatcaaccaa aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg
     9421 gggtcgagaa tgcatgactt ttctgttttt ttacgggtaa gccgcaacga ccattgacag
     9481 acccgggcag gctgatattc tccgcagcca gacttttttc gccagacacg actttgtaga
     9541 aattgtttta caaaaatggc gatgcaatct gcggcgcggg gtgggatgat agcccacttt
     9601 cgaaagccga ttcggcgaca attgtcccga aatcgcctct ggttcaggta tactgacaga
     9661 ccatttttat ctatttgatt cacccagggg gaaaacttgc agggtaaagc actccaggat
     9721 tttgttatcg acaaaattga tgacctcaaa ggtcaggaca tcatcgcctt agacgttcaa
     9781 ggcaaatcca gcatcaccga ctgcatgatc atctgtacgg gtacgtccag ccgtcatgtt
     9841 atgtccattg ctgaccacgt tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc
     9901 gtagaaggtg aaaacagcgc cgactggatt gtcgtggatt tgggcgatgt gattgtccat
     9961 gtcatgcagg aagagagccg tcgcctgtat gaactggaaa aactctggag ttaatgcgtg
    10021 aagctgcaac ttgtcgccgt gggaacgaaa atgccggact gggtacaaac cggttttacc
    10081 gagtacctgc gtcgttttcc gaaagatatg cccttcgagc tgattgaaat tccggccgga
    10141 aaacgcggca agaatgcgga catcaagcgc atactcgaca aagagggtga gcagatgttg
    10201 gcggccgcag gcaaaaaccg cattgtcacc ctcgatattc caggcaagcc ctgggataca
    10261 ccgcagttag ccgctgagct ggaacgctgg aagctggatg gtcgcgacgt cagtctactg
    10321 attggcgggc ctgaagggtt gtcgcctgcc tgtaaagcgg cggcggagca gagctggtcg
    10381 ctgtcggcgc ttaccctccc ccatccgctg gttcgcgtgc tggtcgcaga gagtctgtac
    10441 cgggcgtgga gcatcaccac caaccatcct tatcaccgtg agtgataagg gagctttgag
    10501 tagaaaacgc agcggatgaa actacagaac tcttttcgcg actatacggc agagtccgcg
    10561 ctgtttgtgc gccgggcgct ggtcgcattt ttggggattt tgctgctgac cggcgtgctt
    10621 atcgccaacc tgtataatct gcaaattgtt cgctttaccg actaccagac tcgctctaat
    10681 gaaaaccgca ttaagctggt gcctatcgcg cccagccgcg gcattatcta cgaccgtaat
    10741 ggtatccctc tggccctcaa ccgcactatc taccagatag aaatgatgcc agagaaagtc
    10801 gataacgtgc agcaaacgct ggacgctttg cgcagcgtgg ttgatctgac cgatgacgat
    10861 attgctgcat tccgaaaaga gcgcgcacgt tcacaccgtt tcacctctat tccggtgaaa
    10921 actaacctga ccgaagtaca agtagctcgc tttgccgtca atcagtaccg ttttccgggt
    10981 gtcgaagtta aaggctataa acgtcgttac tatccttacg gttcggcgtt gacccacgtc
    11041 atcggctatg tgtcgaaaat caacgataaa gacgtcgaac gcctgaataa tgacggcaaa
    11101 ctggccaact atgcggcaac gcatgatatc ggtaagctgg gcattgagcg ttactatgaa
    11161 gacgtgctgc acggtcagac cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt
    11221 atccgccagt taaaagaagt accaccgcaa gccggacacg atatttacct gacgctggat
    11281 ctcaaactcc agcaatatat tgaaacgctg ctggcgggta gccgcgcagc tgtggttgtc
    11341 accgatccgc gtacaggtgg ggtgctggcg ctggtttcca cgcctagtta tgacccaaac
    11401 ctgtttgttg acggtatctc cagcaaagat tattccgcct tgttgaacga cccgaataca
    11461 ccgctggtga accgcgccac acagggggtt tatcctcccg cgtctacagt taaaccctat
    11521 gtggcggttt cggcattgag cgccggggtg atcacgcgca atacgacgct gtttgaccca
    11581 ggctggtggc aactgccagg ttcggaaaaa cgttatcgtg actggaaaaa atggggccac
    11641 gggcgtctga atgtcacaag atcgctggaa gaatctgcgg ataccttctt ctatcaggtg
    11701 gcctacgata tggggatcga tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat
    11761 tacaccggta tcgacctggc ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa
    11821 cagaaacgct ttaaaaaacc gtggtatcag ggtgacacca ttccggttgg tatcggtcag
    11881 ggttactgga cagcgacccc aatccagatg agtaaggcac tgatgatcct gattaatgac
    11941 ggtatcgtga aggttccgca tttgctgatg agcaccgccg aagacggcaa acaggtgcca
    12001 tgggtacaac cgcatgaacc gcccgtcggc gatattcatt ccggttactg ggagctggcg
    12061 aaagacggta tgtacggtgt tgctaaccgc cctaacggta cggcgcataa atactttgcc
    12121 agcgcaccgt acaaaattgc ggcgaaatct ggtaccgctc aggtcttcgg tctgaaagcg
    12181 aacgaaacct ataatgcgca caaaattgcc gagcgtttac gtgaccacaa actgatgacc
    12241 gcctttgcgc catacaacaa tccgcaagtg gctgtcgcca tgattctgga gaacggcggc
    12301 gcgggtccgg cggttggtac gctgatgcgc cagatcctcg accacattat gctgggtgat
    12361 aacaacaccg atctgcctgc ggaaaatcca gcggttgccg cagcggagga ccattaatca
    12421 tgacggataa tccgaataaa aaaacattct gggataaagt ccatctcgat cccacaatgc
    12481 tgctgatttt actggcattg ctggtttaca gcgccctggt tatctggagc gccagcggtc
    12541 aggatattgg catgatggag cgcaaaatcg gccaaatcgc gatgggtctg gtcatcatgg
    12601 tggtgatggc gcaaattcct ccacgcgttt atgaaggctg ggccccctat ctctatatca
    12661 tctgtattat tttgctggtg gcggtagatg ctttcggtgc catctctaaa ggtgctcaac
    12721 gctggctgga cctcggtatt gttcgttttc agccgtcgga aattgccaaa atagccgtac
    12781 cactgatggt tgcgcgcttt atcaaccgcg acgtttgccc gccatcgttg aagaacactg
    12841 gcatcgcgct ggtgctgata tttatgccca cgctgctggt ggctgcacag cctgacctgg
    12901 gaacatcaat cctcgttgcg ctttccggtc tgtttgtact gttcctctct ggccttagct
    12961 ggcgtctgat tggcgtcgca gtagtgctgg tagcggcgtt cattccgatt ctgtggttct
    13021 tcctgatgca tgattaccag cgccagcgcg taatgatgct cctggacccg gaatcagacc
    13081 cactaggcgc gggctatcac attattcagt ctaaaattgc tattggctcc ggcggattac
    13141 gcggcaaagg ctggctgcac ggcactcagt cacagcttga atttctcccc gaacgccata
    13201 ccgactttat cttcgcggta ctggcggaag agctgggatt agtgggcatt ctgattctgc
    13261 tcgctctcta cattctgctg atcatgcgcg ggctgtggat agccgccaga gcgcaaacca
    13321 cctttggtcg cgtcatggct ggtggcttaa tgctgatatt attcgtttat gtcttcgtaa
    13381 atattggtat ggtaagcggt attctgccgg ttgtaggggt tccgctccca ctggtcagtt
    13441 atggaggatc ggcgctaatt gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca
    13501 cccacaggaa aatgttgtcg aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc
    13561 gggatctgca tcgcggcagg aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag
    13621 acggtaagtg taccgcagcc tgcggtatgt aacggcccca tagttgaaat tagcggggcg
    13681 gacccgcgtt tcgaaccact gaacgcgacg gcaaatcagg attaccagcg cgacggtaaa
    13741 agctacaaaa tcgtgcagga tccgtctcga tttagccagg cgggactggc ggcaatctat
    13801 gatgccgaac ctggcagtaa cctgacggcc tctggcgaag ctttcgatcc gacacagttg
    13861 acggcggccc atccaacgct tccgatcccc agctacgcca gaatcactaa cctggctaac
    13921 gggcgaatga tcgtggtgcg cattaatgat cgcggcccct acggcaacga ccgcgtaatt
    13981 tcgctttctc gcgcagcagc tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc
    14041 gatccgatta ttgtcgccca ggatggttcg ctttctggtc ctggtatggc ttgtaccaca
    14101 gtcgccaaac agacttacgc cctgcctgca cctcccgatt taagcggtgg cgcgggggca
    14161 agttcagtgt ctggcccgca gggtgacatt cttccggtca gtaactcgac gctaaaaagc
    14221 gaagatccga ccggcgcgcc ggtaaccagc agcggtttcc tcggcgcacc aacgacctta
    14281 gcgcctggtg tactggaaag cagcgaaccg acacctgcac cacagcccgt tgttacagct
    14341 ccgtcgacaa cgcctgcaac ctcgcctgca atggtgacac cgcaagccgc ctcgcaaagc
    14401 gccagcggca actttatggt gcaagtcggg gccgttagcg atcaggctcg tgcgcaacag
    14461 taccaacagc aactgggaca gaagttcggc gtccccggtc gcgtaactca aaatggcgcg
    14521 gtctggcgta tccagcttgg cccattcgcc agtaaagccg aagccagtac cttgcagcaa
    14581 cgtttgcaaa ccgaagccca attacagtca tttattacca ccgcgcagta gcgtaaagca
    14641 ggcatctgat gtgtcaattt ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg
    14701 cctgccggta gtgcatttgc tatagtaggg cacttttttt aattccatca cggatgtcgt
    14761 agttcagacc atgaatacca ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac
    14821 ggctctttgc acagccttta tctctgctgc acatgccgat gacctgaata tcaaaactat
    14881 gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc ctgattgact ataactccgg
    14941 caaagtgctc gccgaacaga acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat
    15001 gatgaccagt tacgttatcg gccaggcaat gaaagccggt aaatttaaag aaactgattt
    15061 agtcactatc ggcaacgacg catgggccac cggtaacccg gtgtttaaag gttcttcgct
    15121 gatgttcctc aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg gtattaacct
    15181 gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt gccgctggta gccaggacgc
    15241 ttttgttggc ttgatgaata gctatgttaa cgccctgggc ctgaaaaaca cccacttcca
    15301 gacggtacat ggtctggatg ctgatggtca gtacagctcc gcgcgcgata tggcgctgat
    15361 cggccaggcg ttgatccgtg acgtaccgaa tgaatactcg atctataaag aaaaagaatt
    15421 tacgtttaac ggtattcgcc agctgaaccg taacggcctg ttatgggata acagcctgaa
    15481 tgtcgacggc atcaaaaccg gacacactga caaagcaggt tacaaccttg ttgcttctgc
    15541 gactgaaggc cagatgcgct tgatctctgc ggtgatgggc ggacgtactt ttaaaggccg
    15601 tgaagccgaa agtaaaaaac tgttaacctg gggcttccgt ttcttcgaaa ccgttaaccc
    15661 actgaaagta ggtaaagagt tcgcctctga accggtttgg tttggtgatt ctgatcgcgc
    15721 ttcgttaggg gttgataaag acgtgtacct gaccattccg cgtggtcgca tgaaagatct
    15781 gaaagccagc tatgtgctga acagcagtga attgcatgcg ccgctgcaaa agaatcaggt
    15841 cgtcggaact atcaacttcc agcttgatgg caaaacgatc gagcaacgcc cgctggttgt
    15901 gttgcaagaa atcccggaag gtaacttctt cggcaaaatc attgattaca ttaaattaat
    15961 gttccatcac tggtttggtt aaaaattgaa cacttgaaag tgtaatttcc gtccccatat
    16021 actaagcatc agtaaaaaaa ctcccgcctt ctggcgggag ttgctattta attacgttac
    16081 gccggagctg acatgaaaac caaacttaac gaactgcttg aattccctac tccttttact
    16141 tacaaagtta tggggcaggc gttacctgag ctggttgatc aggtggttga agtggtacag
    16201 cgccatgcgc caggtgacta caccccaacg gtaaaaccaa gcagcaaagg caactaccac
    16261 tcggtatcta tcactatcaa cgccactcat atcgagcagg ttgaaacact gtatgaagaa
    16321 ctgggcaaaa tcgatattgt ccgcatggtt ctgtaactcg cttctccgtt acccggctcc
    16381 tggtcgggta actcccctct cctcgctgtg atatactttt cccccacttt tactcattct
    16441 ccacggagat gccgttttgt atcaggataa aattcttgtc cgccagctcg gtcttcagcc
    16501 ttacgagcca atctcccagg ctatgcatga attcaccgat acccgcgatg atagtaccct
    16561 tgatgaaatc tggctggtcg agcactatcc ggtattcacc caaggtcagg caggaaaagc
    16621 ggagcacatt ttaatgccgg gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt
    16681 gacttatcac gggccgggac aacaggtgat gtatgtgttg cttaacctga aacgccgtaa
    16741 actcggtgtg cgtgaactgg tgaccttgct tgagcaaaca gtggtgaata ccctggctga
    16801 actgggtata gaagcgcatc ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa
    16861 aatttgctca ctgggtttac gaattcgacg cggttgttca ttccacggtc tggcattaaa
    16921 cgtcaatatg gatctttcac catttttacg tattaatcct tgtgggtatg ccggaatgga
    16981 aatggctaaa atatcacaat ggaaacccga agcgacgact aataatattg ctccacgttt
    17041 actggaaaat attttagcgc tactaaacaa tccggacttc gaatatatta ccgcttaatt
    17101 ccatacatca atggcccaat ttacactggg tcattactca taatacctgc taatgcttta
    17161 catctccttc gcgtttgtat attttcttta tcacttgcag aaatgcggtg ccacgccttg
    17221 ctccgtaact caccccattg attaacctat tgaaatagtt gattatggtg agcaaaagcg
    17281 ttccgttcca attagacata agattattgt aattttttaa atattatttt tccatgaata
    17341 aaattggagt aagtgcgtgg atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc
    17401 gtcagaaggt aaaccacaaa tatttacaac cttacgaaat attgatctta accttctgac
    17461 tatttttgaa gctgtatatg tacataaagg gatcgttaat gcagcgaaag tgcttaatct
    17521 gaccccctcg gcaatcagtc agtctattca gaaactgcgc gttatattcc ctgacccatt
    17581 gtttattcgc aaaggccagg gtgtcactcc taccgcattt gcgatgcatc tacatgagta
    17641 tatcagtcag ggccttgagt ccattcttgg cgcgctggat atcgaaggaa gctatgataa
    17701 gcaacaaacg ataactattg cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat
    17761 ctatcgggcg attaaaactc actatccgca gcttttactg cgcaacccgc ccatcagcga
    17821 cgcggaaaac caactcagtc agtttcaaac cgatctcatc atcgataaca tgttttgcac
    17881 caatcgtacg gtgcaacatc atgttctgtt caccgacaat atggtgttaa tttgccgcga
    17941 gggaaatcca ctactctctt tagaagatga cagagagact atcgacaacg ctgcgcatgt
    18001 acttctgtta ccggaagagc aaaatttcag cggtctgcgg cagagagttc aagaaatgtt
    18061 tccggaccgg caaatcaatt tcaccagcta caacattttg acaatcgctg cactggttgc
    18121 caacagtgac atgttagcga ttattcccag ccgtttttat aacctgttta gccgctgctg
    18181 gccgctggaa aaactgcctt ttccgtcctt aaatgaggag caaatagatt tctctatcca
    18241 ctacaacaaa ttcagcctgc gtgatcccat cctgcacgga gtaatagatg ttatccgtaa
    18301 tgcattttga aaaagtattg ttacagccag atcacagcgc agaacgataa aatggcacaa
    18361 tccaacaaca attttacatt ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga
    18421 tatactgcca gtcgttaatt caaaaatagt tgataattac aacaatctat tgaattgaaa
    18481 cgctttcctt cgtaattcgc aactggaaca cgcacgctat gagtaaaccc attgtgatgg
    18541 aacgcggtgt taaataccgc gatgccgata agatggccct tatcccggtt aaaaacgtgg
    18601 caacagagcg cgaagccctg ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg
    18661 actctacacg tatccagggc atcaaagccg caatgcgcaa aaatggcctg cattctgtct
    18721 gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa ccacggcaca gcaacgttta
    18781 tgatcctcgg cgctatttgt acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc
    18841 cggtagctcc tgatgccaat gaaccagtga aactggcgca gaccattgcc gatatggcgc
    18901 tgcgttatgt ggttatcacc tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc
    18961 actttgcgga ttgcattact gccattcggg aaaaaagccc gcaaatcaaa attgaaactc
    19021 tggtgccgga tttccgtggt cgtatggatc gtgctctgga tattctgact gcaacgccac
    19081 cagatgtgtt caaccataac ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg
    19141 gtgcagatta caactggtcg ctgaagctgc tggaacgctt taaagaagcg catccggaaa
    19201 tcccgaccaa gtctggtctg atggtgggac tgggtgaaac caatgaagaa attattgagg
    19261 taatgcgcga cctgcgccgt catggtgtga cgatgttaac gctggggcaa tatttgcagc
    19321 caagccgcca tcacctgccg gttcaacgtt acgttagccc ggatgagttc gacgaaatga
    19381 aagccgaagc gctggcgatg ggctttaccc atgctgcatg cggtccgttt gtccgctctt
    19441 cttaccacgc cgatttgcag gcgaaaggga tggaagttaa gtaatcctgt aataattact
    19501 tacatctgtc tgataaaaaa cccgcttttt gaagcgggtt ttttgtatcg aacaagatat
    19561 tgagggagcg tcctgctcgc cacgtcactc tttatgagag agcttttcag cctgaagatc
    19621 aacgtctgcg ccttttttcg ccgcagcatc gtcatcattc atcgccttct tgaacccttt
    19681 aatggccgct ccaaggtctc cgcccagcgt acgtaacttc ttagtcccaa acagcagaac
    19741 gaccagcgcc gcaactacca gcagtttggt aatactaatc tcacccatag ataccttctt
    19801 gacatataac cggcaacaga acgcgcctta aaaaccagag aatattaacg gtcattcgac
    19861 gcgcgcacac aatagcaatc tgtaacaagg tgaatcaagc gccgagtaga aaaacatcat
    19921 aataattgcg gcggcgcaaa gcgacggttg tttaagacgg gcagttgcgc gcgcacctga
    19981 cgcacacgtt cgggcgtcac ttccgccata atgagtgcag gcatttctga cgctgccgca
    20041 atggtgacgc caaagggatc tataatccgg ctttgaccga tatttttgtt cccgcactcc
    20101 cccgccgcca ccatataaca ggtggtatcc agcgcacgag cggcaagcaa cgttgaccag
    20161 tgatgctctt tgagcggccc gcgaacccag gcggcaggaa gtaccaggat ttcagctccc
    20221 tgtaatgcct gtgccagcgc cagctctgga aagcgtaagt cataacaggt catcagaccg
    20281 accttcatcc cttccacctc cagtaacgga gcgatttcat taccagcatc aacacggcgt
    20341 gattcctgaa tggcaaatgc atcatagaga tgcagtttgg cataacgggc gacgatgttt
    20401 cctgcctgaa gtgccaccag catattccat gcgcgccccg gcgttgaagg aacatgaatc
    20461 gtcagaattg tcgtcatcat gttacgttta ctttctcgcc gtaaacgtcc gaggaattcg
    20521 ccttccagca gctgtgctga tttaaccgat agatctgcat catgatcatc gcgcgccagc
    20581 aatgcttccg gcaggacaaa cagcgatacg tcgttttccg ccgcctgcgc catcaacgag
    20641 gcacaaatct cagcgttctt ttcccacaca gatgtaacag caaactgtcc tgcggcaacc
    20701 agcattgcca cctcctgtca gagtcgtaaa attcgccaac gacgattatt cggcgttaca
    20761 cttatcactc atacaaatca aatagcagga ttttgcagtg ttacaacttc ttttagcagt
    20821 ttttattggc ggtggtacgg gaagcgtggc gagatggctg ttaagtatgc gatttaaccc
    20881 actgcatcag gcgattccgt tggggacgct gacagcaaac ctgattgggg cattcatcat
    20941 aggaatggga ttcgcatggt tcagcaggat gacgaacatt gatccagtgt ggaaagtatt
    21001 aatcaccacc ggattttgtg gcggtctaac aaccttctca acattttcgg cagaagtggt
    21061 gtttttgtta caagagggcc gctttggctg ggcattactg aacgttttcg tcaaccttct
    21121 ggggtctttt gccatgaccg cactggcatt ctggctgttt tcggcctcaa ccgcacacta
    21181 aagaccaaaa aaaacccgct gattaagcgg gttttgaatt cttgctgacg tatcttacag
    21241 agcgattacg tttgcagcag aagggccttt ggcaccgtta gtgatttcga actctacgcg
    21301 ctgaccttca gcaagagttt taaaaccatt agtctggatt gcagagaagt gtacgaacac
    21361 gtctttgctg ccgtcttccg gagtaatgaa accgaatcct ttggactcat taaaccactt
    21421 aacgttacct ttaatcttag acatcaaaat tacctttaca tgaaaaatag acacaaatgc
    21481 tgtgtcggtt accagtacac caattgtggt acgctttgtc cagtgcaact ttactaaaaa
    21541 gtgataaaag tcgcgttatt tttgatgtta cttattttag ctattgattt taaagaagtt
    21601 actaaaactt catttgcctc aaaactgaaa gcgcatccag gcaaagtaca cattgccatt
    21661 gttgtaggta cccggaatgt aggtcatctg aaaagtcact gggccataac ccacggaggc
    21721 caatggcagt agaaccggga gagggatgta attccagtta tcgcgtgccg ttacgccagc
    21781 ggtgaatccc agacctaaat gaaaattttc atccgccagc ggtcgccagg tactttccca
    21841 tccgtatccg gcaatcggtt cccatttgtt ccacgagtcc ttaaatgcca tggcatacag
    21901 gccatgccag tttccttttt catcccaacg cgacaggcca aaaccgccac cccacggtcg
    21961 ctcgttatag cgatcggttt tttctttgtc gtaagcgaaa cgtgcatgcc aggtgatggc
    22021 aggaatatat aaatcataat gttcaggctg ttgccaggtt tgtgcaatat tttctctaaa
    22081 cgttgtcatc cactcatctg cgttagcaaa aactttacca acgctgatta actgaataaa
    22141 aacaaaggaa aagatagcga catatttact cacgttcatt tgtgaccata aaacatttat
    22201 caaaaatcta ctactagcat agcaaagcta caattaacat aaccttaata gacccaacat
    22261 aaagaataat ctgaatagct gctctattta tttccgcttg ttcaagttaa atatttccgc
    22321 gcatcgatta aagatgatgc gtcgttttct gcgatgggaa tagtcaaaaa agaaaaacca
    22381 agtctttttt gat
//
LOCUS       DAFNBJ010000079        22173 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000079 DAFNBJ010000000
VERSION     DAFNBJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.079"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            89..961
                     /locus_tag="L2X49_002804"
     CDS             89..961
                     /locus_tag="L2X49_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBN4797870.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIKVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQCSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSDSGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1021..2319)
                     /gene="eno"
                     /locus_tag="L2X49_002805"
     CDS             complement(1021..2319)
                     /gene="eno"
                     /locus_tag="L2X49_002805"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBN4797871.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2407..4044)
                     /gene="pyrG"
                     /locus_tag="L2X49_002806"
     CDS             complement(2407..4044)
                     /gene="pyrG"
                     /locus_tag="L2X49_002806"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBN4797872.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4272..5063)
                     /gene="mazG"
                     /locus_tag="L2X49_002807"
     CDS             complement(4272..5063)
                     /gene="mazG"
                     /locus_tag="L2X49_002807"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBN4797873.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5134..5469)
                     /gene="mazF"
                     /locus_tag="L2X49_002808"
     CDS             complement(5134..5469)
                     /gene="mazF"
                     /locus_tag="L2X49_002808"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HBN4797874.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5469..5717)
                     /gene="mazE"
                     /locus_tag="L2X49_002809"
     CDS             complement(5469..5717)
                     /gene="mazE"
                     /locus_tag="L2X49_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HBN4797875.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5795..8029)
                     /locus_tag="L2X49_002810"
     CDS             complement(5795..8029)
                     /locus_tag="L2X49_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBN4797876.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8077..9378)
                     /gene="rlmD"
                     /locus_tag="L2X49_002811"
     CDS             complement(8077..9378)
                     /gene="rlmD"
                     /locus_tag="L2X49_002811"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBN4797877.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9435..12191
                     /gene="barA"
                     /locus_tag="L2X49_002812"
     CDS             9435..12191
                     /gene="barA"
                     /locus_tag="L2X49_002812"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBN4797878.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRNGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12423..13763)
                     /gene="gudD"
                     /locus_tag="L2X49_002813"
     CDS             complement(12423..13763)
                     /gene="gudD"
                     /locus_tag="L2X49_002813"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBN4797879.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAKRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13784..15124)
                     /locus_tag="L2X49_002814"
     CDS             complement(13784..15124)
                     /locus_tag="L2X49_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBN4797880.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15126..16478)
                     /gene="gudP"
                     /locus_tag="L2X49_002815"
     CDS             complement(15126..16478)
                     /gene="gudP"
                     /locus_tag="L2X49_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBN4797881.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16913..17362)
                     /locus_tag="L2X49_002816"
     CDS             complement(16913..17362)
                     /locus_tag="L2X49_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBN4797882.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17380..18162)
                     /gene="truC"
                     /locus_tag="L2X49_002817"
     CDS             complement(17380..18162)
                     /gene="truC"
                     /locus_tag="L2X49_002817"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBN4797883.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18162..18491)
                     /locus_tag="L2X49_002818"
     CDS             complement(18162..18491)
                     /locus_tag="L2X49_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBN4797884.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19113..19658)
                     /gene="syd"
                     /locus_tag="L2X49_002819"
     CDS             complement(19113..19658)
                     /gene="syd"
                     /locus_tag="L2X49_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBN4797885.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19726..20574
                     /gene="queF"
                     /locus_tag="L2X49_002820"
     CDS             19726..20574
                     /gene="queF"
                     /locus_tag="L2X49_002820"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBN4797886.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20686..22050
                     /gene="ppnN"
                     /locus_tag="L2X49_002821"
                     /gene_synonym="ygdH"
     CDS             20686..22050
                     /gene="ppnN"
                     /locus_tag="L2X49_002821"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBN4797887.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5453 a         5895 c         5248 g         5577 t
ORIGIN      
        1 ttttctgtat tagtttttaa tgataacgaa tctaaaatta ccgatgccgc cgtcaattta
       61 tttatccaga tttaatgaga aatttataat gcgctatttc atactaatgt tcactttcgt
      121 gtgttccttt gttgcagccc aaccaactat tgtcccacaa ttacagcaac aagttacgga
      181 tcttacgagt agcttaaact cacaagaaaa gaaagaactg acgcacaagt tagaatctat
      241 tttcaataat acccaagtgc aaattgctgt attaattgtt cccacaacca aagacgagac
      301 aattgaacaa tatgctacaa gagtttttga caattggcgt ttaggagatg ccaaacgtaa
      361 tgatgggata ctgatcattg ttgcctggtc agatcgtact gtccgcatca aggtaggtta
      421 tgggctggaa gaaaaggtaa ccgatgctct ggctggagat atcatccgta gcaacatgat
      481 acccgcattt aaacaacaaa aattagctca gggattagag ctagctataa acgctttgaa
      541 taaccaactc acttcacaac accaatatcc gactaatcct tcagaaagtg aatcagcgtc
      601 ttccagtgat cattattact ttgctatttt ttgggtattt gcagtgatgt tcttcccttt
      661 ctggtttttt catcaatgca gtaatttttg tcgcgcatgt aaaagtggag tctgtatttc
      721 agcaatctat cttttagatt tattcctgtt ctccgataaa attttttcca ttgctgtatt
      781 ttccttcttt tttactttca ccatatttat ggtctttacc tgtttatgtg tacttcagaa
      841 aagagcatct ggtagaagtt atcattcaga caacagcggt tccgctggag gctcagatag
      901 cggcggattt agcggcgggg gcggttcttc tggaggtggc ggcgcatccg gccgctggta
      961 actcaaccat aaaaaatgcc agcccggagg ctggcatttt taaatcagat aaagtcagtc
     1021 ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc ccagagcttc
     1081 ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca tagaaccagt
     1141 tttgatctgg cctgcagcag taccaacagc caggtcagcg atggtagcgt cttcagtttc
     1201 gccagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct tgattgcagc
     1261 cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt tagcgatacc
     1321 tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt caccaaccag
     1381 ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt cagattcgtc
     1441 cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca ggaagtgagt
     1501 gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt taccatcttt
     1561 gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca gttcataacc
     1621 agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac ccaggttcgg
     1681 cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca gaacttttgc
     1741 caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt tcgcgccaac
     1801 cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac caccgttgat
     1861 gatgttcatc atcggaaccg gcatagagta tttgcccgga gtaccgttca gttcagcgat
     1921 gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag ccagagatac
     1981 agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca ggtcgatcat
     2041 gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct gagcgatcgg
     2101 gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg atttgtcgcc
     2161 atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag cagctgccat
     2221 accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac cacgggagtc
     2281 gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct caagtcacta
     2341 gttaaactga aactccagac aaacaacgcg tacccagggt acgcgttgcc gctctaactt
     2401 ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa agcctgcaaa
     2461 cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc aagccacgaa
     2521 ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg aacggcccgc
     2581 aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt cgtaacggtg
     2641 acgatgacgc tcaacaattg tcggcgcatt gtacagctgg cgaaccaggc tatcgtcaac
     2701 caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct tctcgctacg
     2761 aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa ccgggtactt
     2821 acagtctggc acaaattccg tagagttggc gttctccatg ttggcaacat ggcgagcgta
     2881 atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat tgttctcacg
     2941 cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac cgccaggtac
     3001 gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat cttgtgaatc
     3061 gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca gtgcttcgat
     3121 cactgattta taagcatccg gcagttcaat gtacttgccg accataccga tggtgacttc
     3181 acttaccggg ttcgcttctt cgaagataac ctgttcccat tcggacagat tcgcttccgg
     3241 gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt tcaacaggcc
     3301 cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt ccggaacatt
     3361 acagaacaat gcaatcttcg cacgttcgtt cgccggaaca gcgcgatctg aacgacaaat
     3421 caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct gagtcggttt
     3481 ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca taaacagagt
     3541 gtgctcacgg ccaatttcaa cagccatctg gcgaatcgct tcgaggaacg gcaaggattc
     3601 gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt cgccaccttc
     3661 cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg ttgcgccgag
     3721 gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg tggtgaagtt
     3781 gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt ccaggtcggt
     3841 ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca tagtacctgg
     3901 atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac gggcttcaag
     3961 aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga ccccgccggt
     4021 cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa agacgatgga
     4081 ataaccagga cgggaaagca gtatacccga acatgacctg tgccacaaac tttcattatc
     4141 cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc tttggggtaa
     4201 atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg gcgcttgacc
     4261 gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac ttcttccatt
     4321 gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg ctccacttcg
     4381 cgaaaacgac gctcaaattt ttcgttcgct ttttgcaatg cgatttccgc ttttgtccct
     4441 aaatgacgag ccagattgac cgtggcaaac agcaggtccc ccatttcctc ctccagttta
     4501 gcctggtcga caacagcctg ccgcgcttcg tacatcacct cgtcgatctc ttcgtagact
     4561 ttatcgacta ccggaccaag cgtcgtccaa tcgaagccaa cgttggcgca acgtttctgg
     4621 attttttgcg cacgcattaa agccggtaaa ctacgaggaa tatcgtccag cgccgaatgc
     4681 tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag cacttcacta
     4741 ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa tttatcgcta
     4801 atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat ttgcgcgtaa
     4861 aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa atcttcacgg
     4921 gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat ggtggcaaat
     4981 gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg cataatagtg
     5041 agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg tcaggtggaa
     5101 acctgtgacc agaatagaag tgagttagta acactaccca atcagtacgt taattttggc
     5161 tttaatgagt tgtaattcct ctggggcaac tgttcctttc ttcgttgctc ctcttgcccg
     5221 ccaggcgata ctttttacct gatcagctaa cgctacgcca tcacgttcct gaccggataa
     5281 aacaacttcg aacggatatc cttttgattg cgttgtacaa ggaacacaca gacacatacc
     5341 tgttttgttg ttgtacatga aaggactcag gacaacagct ggacgatgtc cagcttgctc
     5401 gctacctttt gtcgggtcaa aatcaaccca aatcagatcg cccatatcgg gtacgtatcg
     5461 gcttaccatt accagacttc cttatctttc ggctctcccc agtcgatatt ctcgtggagg
     5521 ttttccggcg tgatgtcgtt gaccagttca gcaagcgtaa atacgggctc tttacgcact
     5581 ggctcaataa ttaatttgcc atccaccagg tcaatcttca cttcatcatc aatattcaga
     5641 ttgagcgcct gcattaacgt agccgggatc cgcaccgccg gtgaatttcc ccaacgcttt
     5701 acgctactgt ggatcataac cctttcctca aaccgctatc atatgtagat acagtatata
     5761 tcaatctaca ttgtagatac gagcaaattt ctgcctaact cccgtgcaac cgacgcgcgt
     5821 cgataacatc cggcacctgg ttgagtttac ccagcacgcg gcccagcact tgcaggttgt
     5881 aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt ggtgtcgcta cggctggcaa
     5941 cgccaagcac gttcaccttc tcgttggcga gaatggtcgt gatatcacgt aacaacccac
     6001 tacgatcatt agccaccacg cggaccacca gcgaatatcc ggcggagtag ctctcacccc
     6061 ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag ttccgccagt tgttcgcaat
     6121 cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa gccgacaatc tcatctccag
     6181 gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt accaacacct tcaactacca
     6241 cacgaccgtt atctttactg cggttttgcg gtgtgtagct tttttgctga agctgtttca
     6301 gcgcggcggc gtcctgctct tcggcactcg gcttattaaa ttgcgattgc aggaagttca
     6361 ccatctgatt gagacggata tccccgccac caatcgccgc cagcaactcg tcgacatcat
     6421 tgaagttgta acgcggcaac agatgttttt ctgcttcttt caggctgatc cccagatgtt
     6481 ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt tttgtcacgg tcctgtttac
     6541 ggaaccaggc gtgaattttc gaacgcccac ggctggttgt gacgtaaccg aggtttgggt
     6601 ttaaccagtc acggctgggg ttcggctgtt tctgggtgat aatttcaatc tggtcgccca
     6661 tctgcagctg gtaggtgaac ggcacaatgc gcccgccaat ttttgccccg atgcagcggt
     6721 gtccgacatc actgtggatg tggtaagcga agtccagcgg cgttgatccc gccggcaaat
     6781 caacgacatc acctttcggc gtaaagacgt acacccggtc gtcaaagacc tgactacgta
     6841 cttcgtcgag catttcgccg gaatcagcca tctcttcctg ccacgcaatc agtttacgca
     6901 gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc agcagccgcg ccctctttat
     6961 atttccagtg cgcagcaaca cccaactctg catcttcatg catctgtttg gtgcggattt
     7021 ggatctcaac ggtttttcca cccggcccca gaaccacggt atgaatagac tgataaccgt
     7081 ttggtttcgg gttagcgacg taatcgtcaa actcatccgg caggtggcga tagtgagtgt
     7141 gcactatccc cagtgcggca taacaatcct gtaaacgctc ggcgacaata cgtaccgcac
     7201 gaacatcaaa cagctcatcg aaggcgaggt tctttttctg cattttacgc cagatgctgt
     7261 agatgtgttt cggacgacca tacacttcgg ctttaacgcc ttcagctttc atctcagcgc
     7321 gcagatgacc gacaaactct tcgatatagt gttcgcggtc gagacggcgt tcatgcaaca
     7381 gttttgcgat gcgtttgtat tcggtcgggt gcagataacg gaagcagtaa tcttccagct
     7441 cccatttcag ttgcccaatc ccaagacggt ttgccaacgg cgcgtagata ttggtgcact
     7501 cttttgccgc cagtacgcgt tcatcttccg gcgcatcttt tacttcccgc agatgagcaa
     7561 tacgctccgc cagtttgatg accacgcagc gaaaatcatc gaccatcgcc aataacatcc
     7621 ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt gtgcgtcgct ttcagctggc
     7681 ggatcgccgc catatcacgc acgccgtgaa taaggttaac gaccgactta ccgacgctct
     7741 cacgcagcac atcttcgctg actacgttgg catccgccag agggaaaagc agcgccgccc
     7801 gcagcgtgtc aatgtccata cttaatgtcg agaggatctc caccatctca acaccacgcc
     7861 acaataacag actggcatcc ggatgcccct gcgtctgttg cagacaatac gcccaggttt
     7921 cggctaagca ctcacacgac ttctggctgg taatacccag acttgcgatc catttttccg
     7981 gatcaaattc accagcctta ttgatatgtg cacttcttac cgcaaccatc gtcctctcct
     8041 ttagggacca gacctgccga aatcggcaaa tcgcaactat ttaacgcgcg agaaaagtac
     8101 catcgattcc agatgtcccg tgtgtgggaa catatccagc atcgccagtc gcgcaatggt
     8161 atatcctgct tttaataacg cttcgctatc ccgagccagc gttgcagggt tacaggatac
     8221 ataaactata cgaataggtt ccagttttat aatttgctgc ataacacctg cggcacctgc
     8281 tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc gcccacggct gctttgtgac
     8341 atcttcttca agattttcgt gataaaacgt cacattctgt aagccattaa gacgcgcatt
     8401 ctgctggcct ttttccacca gcgccggaac accttctaca ccgaccacac tggcagcttg
     8461 tgtcgccaat ggcagtgtaa agttgcccat accgcagaac agatccagta cgcgatcttc
     8521 aggttgcaca tccagccatt ccaacgcacg cgctaccatt ttttggttca cacccgcatt
     8581 gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac ccgtttgagt cataccaggg
     8641 catctcacca gagacggttt cgagtatctc actatcgggg gcgagataca gatccaggcc
     8701 ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc gaacttagcg gtgcggtatg
     8761 gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt tcaacatgac caagatggcg
     8821 catagcttgt aagctgccca gacatgccct gactttgggc agcaatgctt caagttgggg
     8881 cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg gagcccgctt tgcgaaaccc
     8941 catctgaagt tgctgtgttt tcggtaagta gttcagactt aaacgcgcgc gacggcgata
     9001 gccccaggga acatcggcga tcacttcaga gacatcgtgt ttcattaatc gggcgagtgc
     9061 cgcacttttg cttcgctgct gtaaatccac gctggcgtgt tgttgctgac agccaccgca
     9121 tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg ctatcgctta accggcgtac
     9181 gactttagcg cgggcatact gttttttatc ttcagtaaca gtaacttccg cgttttcctg
     9241 cggcaataat ccggggataa atagcgtttt gccgttatgt cgcgccacgc cctgaccaaa
     9301 agagtcgagg tcgttgactg aaacggttat gatctgacgc gtcgtcgtgc gtcgttttgc
     9361 agagtagaat tgcgccattg gcgagacttt ctcaatttaa cagtgtgacc ttaattgtcc
     9421 cataacggaa ctccatgacc aactacagcc tgcgcgcacg catgatgatt ctgatcctgg
     9481 caccgaccgt ccttattggt ttattgctga gtatcttttt cgtcgtgcat cgctataacg
     9541 acttgcagcg tcaactggaa gatgccggtg ccagcattat tgagccgctt gcagtttcta
     9601 ctgaatatgg catgagcctg caaaatcgcg aatctatcgg tcagttaata agcgtactgc
     9661 atcgtcgcca ttccgatatt gttcgcgcga tttcggttta tgatgaaaat aaccgactct
     9721 ttgtcacctc caattttcat cttgatccct catcaatgca gctcggcagc aacgtgccgt
     9781 ttcctcgcca gctcactgtc actcgtgacg gcgatattat gatcctccgc acgccgatta
     9841 tttctgagag ttactccccc gacgaatcgc ccagtagcga tgccaaaaat agtcaaaata
     9901 tgttgggata tattgcgctg gagctggatc ttaaatcggt tcgcttgcag caatataaag
     9961 agatctttat ttccagcgtg atgatgctgt tttgtatcgg tattgcgctt atttttggct
    10021 ggcgcttaat gcgcgatgta accggtccga ttcgcaacat ggtgaatacc gtcgaccgca
    10081 tccgtcgcgg gcaactcgac agccgggtgg aaggatttat gctcggcgag ctggatatgc
    10141 tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc ttatcacgaa gagatgcagc
    10201 acaatatcga ccaggcgacg tccgatctgc gtgaaacgct ggagcagatg gaaattcaga
    10261 acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc ggcgcgtatt aaatccgagt
    10321 ttctggcaaa tatgtcacac gagctgcgta caccactgaa tggtgttatt ggctttaccc
    10381 gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga tcacctgaat acgattgaac
    10441 gttcggcaaa taatttgctg gcaattatta atgatgttct cgacttctcg aaactggaag
    10501 caggtaagct gattctggaa agtattccat tcccactacg cagcacgctg gatgaagtcg
    10561 ttactctgct ggcacattct tctcacgata aagggttgga actgacgctc aatattaaaa
    10621 gcgacgtgcc tgataacgtg atcggcgacc cgctgcgatt acagcaaatc atcactaacc
    10681 tggtggggaa tgcaattaaa ttcaccgaga atggcaacat tgatattctg gtagaaaaac
    10741 gtgcgctgag taataccaaa gtgcagattg aagtgcagat tcgggatacc ggcattggta
    10801 ttcctgaacg cgatcaatcg cgcttattcc aggccttccg acaggctgat gccagtattt
    10861 cccgccgtca tggtggcacc gggctggggc tggtgattac acaaaaactg gttaatgaaa
    10921 tgggcggcga tatttcgttc catagccagc cgaatcgcgg ttcaactttc tggttccaca
    10981 ttaatctcga tctgaacccg aacattatta tcgaagggcc atccacccag tgcctcgcag
    11041 gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc gcaatgcacg ctggatattt
    11101 taagtgaaac gccgctggaa gtggtttata gcccaacgtt ctccgcgctg cctcccgcgc
    11161 attacgacat gatgttgtta ggcatcgcgg tgaccttccg cgagccgcta acaatgcaac
    11221 atgagcgatt agcgaaagcg gtatcgatga ccgatttcct gatgctggca cttccttgcc
    11281 atgcacaagt caatgctgaa aaactcaagc aagatggtat cggcgcgtgt ctgctgaaac
    11341 cattaacacc tacacgcctg ttgcctgccc tgacggaatt ttgtcatcac aaacaaaaca
    11401 cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac agtcatggcg gttgatgaca
    11461 accccgctaa cctgaaactt atcggcgcat tgctggaaga tatggtgcaa catgtggaac
    11521 tttgcgatag cgggcatcag gcggttgaac gggcgaaaca gatgccgttc gatttgatct
    11581 taatggatat tcaaatgccg gacatggatg gcattcgggc ctgcgaactc atccaccaac
    11641 tcccgcatca gcaacaaacg ccggttatcg cggtaacggc gcatgcaatg gccgggcaaa
    11701 aagagaagct gcttggcgca gggatgagcg attatctggc gaaaccgatt gaagaagagc
    11761 gattgcataa tttgttgttg cgctacaagc ctggcagcgg tatttcctct cgcgtcgtga
    11821 cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac cctcgactgg caactggcac
    11881 tacgccaggc agcaggaaaa accgatttag cgcgcgatat gctgcaaatg ttactcgatt
    11941 tcctgcctga agttcgcaac aaagttgagg aacagctggt tggagaaaac ccggaaggcc
    12001 tggttgattt gattcataaa ctgcatggca gttgcggcta tagcggtgtg ccacgtatga
    12061 agaatctctg ccaactgatc gaacaacagc tacgtaacgg tactaaagaa gaagatttgg
    12121 aaccggagct gctggaactg ttggacgaga tggataatgt cgcgcgcgaa gccagcaaaa
    12181 ttctcgggta atggatgtct tgtcgagtag caatcggctt tttatgacgc tggtttcaga
    12241 ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt aacgccttat ccggcctaca
    12301 aaaccaatta aattcaatga attaaaaaat gatgtagacc tgatacatag cacaacaggc
    12361 tattttgcgt ttagcatcag tctcaaaccg gctccagata gagccggttt tggttttctg
    12421 tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc agccaggaat cagatactgc
    12481 attcccatcg catcgtcacg cgcgccaagc ccgtgtttct gatacagctc atgggctttc
    12541 atcacttgat ccatatcgat ttctacaccc agccccggtt tttctggcac ctgtaccagc
    12601 ccgcctttga tctcaaacgg ttctttggtc aggcgctgat tgccttcctg ccaaatccag
    12661 tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg caacatgggt aaacatcgcc
    12721 agggaaatat cgaagtggtt gttagagtgt gaaccccagg tcaggccaaa ttcatggcac
    12781 atttgcgcca cacgtaccga accttgcatt gtccagaaat gcggatccgc cagcgggata
    12841 tcaacggatt gtaacgagag cgtatggccc atctgccgcc agtcggtggc gatcatattg
    12901 gttgcggttg gcaggccagt tgcgcgacgg aactctgcca tcacttcacg cccggagaaa
    12961 ccttgctccg caccacacgg atcttctgca taagccagtg accctttcag gtatttacca
    13021 attttaatcg cttcgttcag cgaccaggca ccgttaggat cgagcgtaat acgcgcctgc
    13081 gggaaacgtt tcgccagtgc cacaatagac tcggcttctt cttccccggc cagtacaccg
    13141 cccttcagtt tgaaatcgtt gaagccatat ttttcatatg ccgcttccgc caggcgcacc
    13201 accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat accagtcgca tgagtcatcc
    13261 ggctggcttt gatacggcag cggtgtggct ttgcgatcac cgacgaagaa cagataaccg
    13321 agcatttcga cttcgctacg ctgttgacca tcgcccagca gcgatgccac gtttaccccc
    13381 agatgctgcc ccagcagatc cagcattgcc gcttctatcc cggtaactac atgaatagtg
    13441 gtacgcaaat caaatgtctg caaaccgcgc ccaccagcat cacgatcggc aaaagtatta
    13501 cgcaccagcg tcagaacgtt tttgtattca cccagcgttt tacctaccac cagcggaatc
    13561 gcatcttcca gcgttttacg gattttctcg ccgccgggaa tttcccctac gccagtgtga
    13621 ccagaattat ctttgataat cacaatatta cgcgtaaaga acggtgcgtg tgcaccactc
    13681 agattcatca gcatactgtc atgacccgcc accgggatga cctgcatttc agtaacaaca
    13741 ggcgtcgtaa attgagaact catatttatg tcctttttca gaatcaatga cggccgaaaa
    13801 cgggacgttt acggtcaaag gtccagccgg ggatcaggta ctgcatcgga cctgcgtcgt
    13861 tacgcgcacc gccaggcaga cgtttatagg cctcatgtgc cttttgtacc tgttcccagt
    13921 ccagttccac gcccagaccg ggcgcatcag gaacggcaat ttttccgttt ttaatctcca
    13981 gcggattttt ggtcaggcga caatcgccct cctgccaaat ccagtgggta tcgatagcgg
    14041 taggattacc cggtgccgcc gcgcccacat gggtaaacat cgccagagag atatcgaaat
    14101 ggttattaga atggcagccc caggtcagcc cccagtcgtc gcaaagctgc gccacacgga
    14161 ctgcaccgga aagcgtccaa aagtgcggat cggcaagtgg aatatctacc gcattgagca
    14221 tcaccgcatg acccatttcg cgccagttgg tggcgatcat gttagtcgcg acgggcaagc
    14281 cggtcgcccg ccgaaactcc gccataactt cacgaccgga gaaaccctgt tctgcgccgc
    14341 atggatcttc ggcataggta agaacatcat tcagcccttt gcatagagaa atggcttcat
    14401 caagtagcca ggcaccgttg ggatcaacgg taatccgcgc atcagggaag cgtttcttca
    14461 atgcacgaac agtgtcgatt tcttgctcgc caggtaacac cccgccctta agtttgaaat
    14521 ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg cacaacggct tcgctgttca
    14581 tcgctttctg atggcgcaac tgataccact catggttgcc cggcgtattt tccagataag
    14641 gaagatcggt tttggtccga tcaccgatat aaaacagata accgaggacg gtaatagtct
    14701 cgcgttgctt gcctggcccc aacagttcgc agaccggaac attcagcgcc ttacctagca
    14761 ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac gcgcaattca aaagtccagg
    14821 cacctttgcc gaaggtatca aaatcggctg cctgattacc tttatgcacc tgctgaacca
    14881 ctttattcag gcgcgcaact tcctggccca gaaccatcgg aatagcatcg accagtgttt
    14941 gataaatcac ctctccgccc ggcgcttcac caatgccggt atgcccggcg ttatcggtga
    15001 gtaccacaat attgcgggtg aaatatgcgt tatgtgcccc accaatatta agcaacatgc
    15061 tatcgtgccc ggcgaccgga atgactttca tatcagtaat aacaggactg gattgtgtcg
    15121 tcatcttatt gccccgcgac aggtttcagc tcgatacgct tgatatcgcc caccagcacc
    15181 aggtagctca gtaccgcgat taaggcatga acaccaacat aaatcagcgc cccattaaag
    15241 gagccagtcg tgccaacgat ataaccaatt gcgattggcg tgacgatgcc agaaatgtta
    15301 ccgaacatgt tgaacaggcc accggaaaga ccgctgatct cttttggcgc ggtatctgcc
    15361 attactgccc aacccagcgc cccgatgccc ttaccgaaga aggccagcgc cataaagccg
    15421 atgatcatcc actcaacgtt gacgtagttg cagaacacca tcaccatcga cagcaacatg
    15481 cccattacga tcggtgtttt acgcgcaatg ttcagcgatc ccgtgcggcg catcagccag
    15541 tcggaaataa tcccacccag cacaccgccg ataaaaccgc aaaccgccgg aacggaagcc
    15601 acaaagcccg ctttcagaat cgacatcccg cgtgcctgca ccagataaac cgggaaccag
    15661 gtaataaaga agtaagtcag ggcgttgata cagtactgac cgatataaac gccgatcatc
    15721 atccgtgacc cgagcagctg tttgatctgc ccccacttca cgctgaacgg cactttaact
    15781 ttagtgtttt gctgatccat attgatcagc gcgccacccg cggcgatata ctccagctct
    15841 ttctgattta cccccggatg ttgatttggc tcgtggatga ctttcaacca gataaagctg
    15901 atgacaatcc ccagaccgcc cataaagaag aagacgtgtg accagcccac ttcatgcgtc
    15961 agccagccca taatcggcgc gaagatcacc gttgcgaagt attgagcgga gttaaaaatc
    16021 gacaccgccg ttcccctttc ctgcgccgga aaccaggccg caacaatgcg actgttgccg
    16081 gggaaagatg gcgcttcagc aagcccgacc aggaagcgca gagtaaacag ggcaacgata
    16141 atgccgaatc cactaaagat atcgacgaag ccttgcagca aggtaaacat cgaccagata
    16201 aagatcgacc agaagtagac gcgttttgaa ccaaaacggt ccagcaacca gccaccaggg
    16261 atctgcccga taacataagc ccatgagaag gcagagaaca catagcccat tcccacggga
    16321 tcaaggccga tatctttggc catttccgaa ccggcaatag aaagcgtagc gcggtcgccg
    16381 tagttgaagg atgtgacgat aaacaacatc accactatcc agtaacgagc attcgtgcgt
    16441 ttttccacac tgctcgcagc ctgacttaaa gaactcattg ttgcactcct gaaaattcgc
    16501 gttagccacg ctcactctgg actgcgacat agccaggaaa tcagaggtga cgtagggtgt
    16561 tttttgccgt ttttataggt cgttcgccga atacggcgcg tgtttatatc tggcaatagc
    16621 agtataaaaa gcgcgccata gcggctcacc gtgcaacaac acaacattaa tgcgttcaat
    16681 gaggcccgat tttggcatta gccctggacg gtggaatcca cttcacggaa atgaaaacaa
    16741 gaacgagaaa ggaagggtta aaacgaagaa ataaagaaga gtatgaaatg gatcgcttga
    16801 ctccaggcaa acgccagtaa aaatccgcgc tatgaagcag tttttactgg catttgcctg
    16861 aaaagattcg attcagcacc gctaaaacga catttaccgc tcgctgaaca tatcaggaca
    16921 acagcgtgcc ccactgttcg acccacggat ttgattccgt ttccggttcc gggttctcac
    16981 tggcgtcaat caacagtatt tcgccaaccc gctgagcgct ctgttcctgc aacaaggcat
    17041 cgaactgttt gccgccattg cagaaattta cataactgct gtcgccgagc gcaatcacgc
    17101 cataacgcag attcggctgg aagcccagac tatctttgat tccctgaaag agcggcacaa
    17161 tgctatcagg aaggtcgccc tgcccggtcg tggacgtaac caccagaaca tacttatcct
    17221 gataaggcag ccagtcgctt aattcaggat cttcaaatac cgttgctttg tggccctgtg
    17281 cggtcagaat tgcttctgct tcttccgcca ctaacagcga atttccgtac atggtgccga
    17341 caaaaatacc aatttccgcc ataccgtttt ctccctggat taggaactta tctctccatc
    17401 ctgacccgat ggcgcactga actcaaccct ttcattttca ggaagcagac cgcgccagcc
    17461 aaattgtgat aacgcctgca tccaggtgtc gtccaggccc gcgtgaatag tcagcggctc
    17521 gccagtaaaa ggatgcgtca atgacaactg actggcatgt agcattaacc gctggaggcc
    17581 aaaatgctca gcaccgctgc gattctggcg taaatcgcca tgtttgctat cgccaataat
    17641 cggatgacgc aaatgggcaa gatgtcggcg aagctgatgt ttgcgtccgg ttttcggctc
    17701 cagttccacc aggccgtagc gcgtggtcgg gtaacgtccg gtcgctaccg gcatttctac
    17761 ggtcgccaga ccgcgataat gcgtcactgc tggctgcgag cctttatctt cgcgggcaaa
    17821 tttatcagcg attttgtcca gttcttccac cagtggataa tccagaactg cttcttccat
    17881 caaccagccg cgcacaatcg catggtaacg tttctggatt tggtgctgtt caaactgttg
    17941 tgccagcagc cgtccggcct cgctggataa tcccatcaac aacacaccag aagtgggtcg
    18001 gtccagacga tgagcggtaa aaacgtgctg gccaatctga tcacgcacgg tttgcatgac
    18061 cactactttc tcgtcgcgat ccagccagct gcggtgaacc agccagccgg agggtttatt
    18121 taccgcaacc agccattcat cctgatagag tatttccagc attagctcgc atcatccgca
    18181 aaaagagcat ccagtttttc cagctcagcc agaataagcg cgcgttgcgg atggtccgtc
    18241 gccagcgcca tttcataata gggtgcaacg gcaaaagcgc ccggtaacgg ctgtttgtta
    18301 tctaacaaat cgtgcattcg cgggatcagc acccactgca accactccag tggttccatg
    18361 gtgtccataa agaacggttg ggtactatta aattgatgcg gctggggttc atcgtttcgc
    18421 cagtgctgat gttcacgcag taacgcttca agcgcctgca actggagacg aacgcggtca
    18481 tgagtggtca taaagcaacc tcaataagaa aaactgccgc gaaggatagc aggaataaaa
    18541 aaagggagca ctgtattcac agcgctcccg gttcgtttcg cagcattcca gctacttttg
    18601 ttgctccctg ctcatccttg acaacttttc ctctggcctt gcggccaatc gttcatcctg
    18661 aactattgct tcctgctcac accaccccga tgtgatactt catcctgaag tgtccctggc
    18721 cttcctgacc caccgaatca tcctgaccgg ttctcattct ccatcctgga ggtgtccttt
    18781 aacgagtcct gcgtcatcct cttcgcttca tccagaagcc tttccctgaa acaccatcct
    18841 ggtgtgtcct gcagaagtgt catcatcctg atgttcgctt cgttgtctga ctccctgtcg
    18901 acgaagatag aatcgtcttt ttcgcgaagt cttacaaggc gcttacagac aatgccttaa
    18961 ggaaaatttc atatgaaagt taagaattaa caatcaacat tttgatttta aatgaaaata
    19021 acgattctta tctttttaag tattcgctta tttcctggcg atctcctaca gagtctgtaa
    19081 gagatctctc acaaccagat ggggtatttg gattacagaa gaggcttaag ttgattaagg
    19141 aattccgcaa gatttgaggc taaatgcgtc cgtttacgtg ttcccagcgt ctctttgcac
    19201 acttcaccgg agagattaca caccgagatg acctctaact cttcttccag cgtcgcgata
    19261 aatagcgtgg gcggcagttt tagccttttc tgagtcacca gatgaccaat cagattttcc
    19321 tgcacccgac ggaagtcgtc ttcactccag gtttgcagca atgtcagttt gatatcgcca
    19381 aactgcgcgt gcatatcccc ggcaaactga gtcgtataaa aggtatgaat tgtgggttgt
    19441 atcacaatat caaaagcgcg ttcaaccgcg tttacatttt gttccccggt aaacggctgg
    19501 ggttgccagt atacggcatc ttcggtagtt gaaataatgc acggtgaagg aacaccgtac
    19561 agttcctcac ttaacggcca gcttttatgc tcttcatgcc acgcatcgca gtagcgtgca
    19621 gtaaaatctt tcagggcttg tgcggtcaaa tcgtccacta atttctctct tcacttaaac
    19681 cagatacact tgtcgtttag tttatctggt ttatgacggt gaaacatgtc ttcttatgca
    19741 aaccatcagg cacttgcggg cctgactctt ggaaaatcaa ccgattaccg ggatacctat
    19801 gacgccagcc tactgcaagg cgttccacgc agcctgaatc gcgacccgct gggtctgaaa
    19861 gcggataacc tgccttttca cggtacggat atctggacgc tgtatgaact ttcctggctg
    19921 aatgcgaaag gtttgccgca ggtcgctgtc ggtcatgttg aacttgatta caccagcgta
    19981 aatctgattg agtcgaagag ttttaagctc tatctcaaca gttttaacca gacgcgtttt
    20041 aataactggg atgaggtgcg ccagacgctg gagcgcgact taagcacttg cgcccagggt
    20101 aaggttagcg tggcgttata tcgtcttgat gaactggaag gccagccgat aggtcatttc
    20161 aatggcacct gcattgatga ccaggatatc actatcgata actatgaatt cactactgac
    20221 tatctggaga atgccaccag cggtgaaaaa gtagtggaag agacgcttgt cagccacctg
    20281 ctgaaatcaa actgcctgat cacccatcaa ccagattggg gttcgatcca aattcagtat
    20341 cgtggacgcc aaattgacag agaaaaactg ctgcgttacc tggtctcatt ccgtcatcac
    20401 aacgagttcc acgaacagtg cgtggaacgc atctttaatg acctgttacg cttctgccag
    20461 ccagagaaat taagcgttta cgcacgttat acccgtcgtg gcggtctgga cattaacccg
    20521 tggcgcagta atagcgattt tgtcccatcg accacaagac tggttcggca ataaattttt
    20581 tctcaatttt gcgtgctgga ttcacgcaga aggttgtgaa aggtcatcag gcagggctat
    20641 tgtaatcaaa gggaatgacg atattcgtcc cataaggagt ttttcttgat tacacatatt
    20701 agcccgcttg gctccatgga tatgttgtcg cagctggaag tggatatgct taaacgcacc
    20761 gccagcagcg acctctatca actgtttcgc aactgttcac ttgccgtact gaactccggt
    20821 agtttgaccg ataacagcaa agaattgctg tctcgttttg aaaatttcga tattaacgtc
    20881 ctgcgccgtg aacgcggcgt aaagctggaa ctgattaatc ccccggaaga ggcttttgtc
    20941 gatgggcgaa ttattcgcgc tttgcaggcc aacttgttcg cggttctgcg agacattctc
    21001 ttcgtttacg ggcaaatcca taataccgtt cgttttccca acctgaatct cgacaactcc
    21061 gtccacatca ctaacctggt cttttccatc ttgcgtaacg ctcgcgcgct gcatgtgggt
    21121 gaagcgccaa atatggtggt ctgctggggc ggtcactcaa ttaacgaaaa cgagtatttg
    21181 tatgcccgtc gcgtcggaaa ccagctgggc ctgcgtgagc tgaatatctg caccggctgt
    21241 ggtccgggag cgatggaagc gccgatgaaa ggtgctgcgg tcggacacgc gcagcagcgt
    21301 tacaaagaca gtcgttttat tggtatgaca gagccgtcga ttatcgccgc tgaaccgcct
    21361 aacccgctgg tcaacgaatt gatcatcatg ccagatatcg aaaaacgtct ggaagcgttt
    21421 gtccgtatcg ctcacggtat cattatcttc cctggcggtg tgggtacggc agaagagttg
    21481 ctgtatttgc tgggaatttt aatgaacccg gccaacaaag atcaggtttt accattgatc
    21541 ctcaccggcc cgaaagagag cgccgactac ttccgcgtac tggacgagtt tgtcgtacat
    21601 acgctgggtg aaaacgcgcg ccgccattac cgcatcatca ttgatgacgc cgctgaagtc
    21661 gctcgtcaga tgaaaaaatc gatgccgctg gtgaaagaaa atcgccgcga cactggtgac
    21721 gcttacagct ttaactggtc aatgcgcatt gcaccagatt tgcaaatgcc gtttgagccg
    21781 tctcacgaga atatggctaa tctgaagctt tacccggatc aacctgttga agtgctggct
    21841 gccgacctgc gccgtgcgtt ctccggtatt gtggcgggta acgtaaaaga agtcggtatt
    21901 cgcgccattg aagagtttgg tccttacaaa atcaacggcg ataaagagat tatgcgtcgt
    21961 atggacgacc tgctacaggg ttttgttgcc cagcatcgta tgaagttgcc aggctcagcc
    22021 tacatccctt gctacgaaat ctgcacgtaa tctcccctcc cccggtgacg tttgttgccg
    22081 ggttttctcg tttttggtca cttactcatc aactcatttc atttgttata tgaatgtttc
    22141 ttaccaccct cacgcgacaa atatcatcac agt
//
LOCUS       DAFNBJ010000080        21880 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000080 DAFNBJ010000000
VERSION     DAFNBJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.080"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..369
                     /locus_tag="L2X49_002822"
     CDS             <1..369
                     /locus_tag="L2X49_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBN4797888.1"
                     /translation="RLRGKDSVSVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFT
                     VSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPR
                     KTLKFKTPKEIIERGVALTD"
     gene            complement(382..639)
                     /locus_tag="L2X49_002823"
                     /pseudo
     CDS             complement(382..639)
                     /locus_tag="L2X49_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            740..1312
                     /locus_tag="L2X49_002824"
     CDS             740..1312
                     /locus_tag="L2X49_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797889.1"
                     /translation="MVDASAQPHIVVTLEPNPWAAFYFDINIANTGNAPAYNVEVVFD
                     PPLVNAEHREKSEIPFSKVSVLKNGQSLTSNLCKYEQIKDQIYNINISWASKPKSNDR
                     ETNEYVYDMATFEGISYLGARSPLTQIAEQIKGIREDWKPIAQGAKKVKADVYTSSDR
                     NEERTYLQEQHDLAIKRRDEKREKRLESGE"
     gene            complement(1361..2185)
                     /locus_tag="L2X49_002825"
     CDS             complement(1361..2185)
                     /locus_tag="L2X49_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797890.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSDKTLLTAIRDYIYGEAWYLVYDLIE
                     FIIERTNSYINLSKHLNSIFKKHGVGYTIINGCITPISNDNEIESVQNAVDNGTDSSR
                     SHFERALQLMTDREQPDYRNSIKESISAIESLCRKITGNDKGTLGACLKAIEEKGYIH
                     SAMKGAFSQLYGYTSDQGGIRHALTEEDVNPTLAEAKFMLVTCSAFSNYLLSKISD"
     gene            complement(3223..3687)
                     /locus_tag="L2X49_002826"
     CDS             complement(3223..3687)
                     /locus_tag="L2X49_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4797891.1"
                     /translation="MKKSLRIQFGERVKELRIATGLSQEAFADRCGFARSYMSRIERG
                     GSNASLDAIEVLANALSVEPWQLLAPGPSEDNDSELLVPYAADGSCFHPGLASTRDGS
                     FGVGDKAAQKRFGTFAEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            3928..4422
                     /locus_tag="L2X49_002827"
     CDS             3928..4422
                     /locus_tag="L2X49_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797892.1"
                     /translation="MTGADFLLPIAAGAAISWASYSVVAHAVSKQHISTLRATVWGAL
                     GTLSLVLGVCAFSAVGAEHQTARKDIVCADDNPECYAKTHHNPVRECKKVMDEKAKFR
                     HVWQESEAPVFNTYLWHDENAKIIQAFGQQAKVVNSLGMLAPLQYFCVFNAKTGEVIA
                     ASFE"
     gene            complement(4483..5328)
                     /locus_tag="L2X49_002828"
     CDS             complement(4483..5328)
                     /locus_tag="L2X49_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797893.1"
                     /translation="MAYTATVIPVMIASPGDVAEERQVIREMIHEWNDINSARSKVML
                     TPIGWETHTSPELGVRPQELINQRLLVDCDLLIGVFWTRLGSPTGNEASGTVEEIHRH
                     LNAGKPAMIYFSSKPVAPESLDREQYESLKLFKTECMQKGLIESFNDLSDFKEKVRRQ
                     LSIIISSSPYLSSLISTINNSPDANTSQSLPESNLSADALSLLKLACVDDSGTIYVIR
                     HLGGTDIQAGNQSFGGSSAREVARWEGALNELLSFDFVIERGAKGQMYYVTHKGWTFL
                     ESLNE"
     gene            complement(5342..5902)
                     /locus_tag="L2X49_002829"
     CDS             complement(5342..5902)
                     /locus_tag="L2X49_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBN4797894.1"
                     /translation="MATFGYGRVSTSQQDTENQRLELGQAGWQIDYWFADVVSGKVPA
                     MQRKAFSEMLNKIRDGETLVVAKLDRLGRDAIDVLQTVRALADRNIKVLVHQLGNTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTQAGLSRAKAEGKKLGRPPKIASEARREILEK
                     KNSGLSISALARQYGVSRATVAAITD"
     gene            6095..6561
                     /locus_tag="L2X49_002830"
                     /pseudo
     CDS             6095..6561
                     /locus_tag="L2X49_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
     gene            7567..7899
                     /locus_tag="L2X49_002831"
     CDS             7567..7899
                     /locus_tag="L2X49_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HBN4797895.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQRHGIDVVSFGNS
                     LHDPDCYYLIRAFVSPKSMTTVLDAFYASDDWRSGPREDIIGNIEASIKTVINLPSES
                     VEGLRVQS"
     gene            complement(8032..9942)
                     /locus_tag="L2X49_002832"
     CDS             complement(8032..9942)
                     /locus_tag="L2X49_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBN4797896.1"
                     /translation="MSQRYKLYRRTSGIYVVRISIPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWHQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSVELPVAQLI
                     QAVMNRNLPVFWLATGQAGFYVAALSEAELDPLDGSYVLNYGEEKGIEGVAKGYLQLT
                     AQPAHLRNIISDGYSEASVFKTAGSDAKGGWFFTSGWPVIKPDTLLINKVHAESLRLD
                     WLAKTTPPVASVHPTVPLAAPAPTIDNEYVNRKYYTKNLSWLCSEYLKHRRKGKVSES
                     AISDIRNYFSFMIEAMGDIQLEDFDRDFLRAYESKLRTIPANRNLVKAKYGVKTLDQL
                     IAKAAECGDKLMTEESVRKYINGIYGAMKWAVADGKLLKSPCENFFPSDDKDERDQDH
                     TDIFEPHEIKAIFSLPWFEAGTVERNAQGRFHQYCPFNYWAPLLGLMTGARVNEIAQL
                     LLSDVLADDGVYYLNLESDNESGKKLKNANARRKIPLHSKLISLGFIDYVNALKDAGY
                     TRLFPELKPHKTKGYGRPVSAWFNESLLAGRLKLERNRSKSFHSFRHSVSTLLKEKGV
                     SSELRAQLLGHVRGETETEVRYSKDLKPIHMIEVVEKIDFSLPDIAEFNIPDGLDAVR
                     DALRRKRGKQTG"
     gene            complement(10033..10117)
                     /locus_tag="L2X49_002833"
     tRNA            complement(10033..10117)
                     /locus_tag="L2X49_002833"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10081..10083),aa:Leu,seq:caa)
     gene            10313..11332
                     /gene="ahr"
                     /locus_tag="L2X49_002834"
     CDS             10313..11332
                     /gene="ahr"
                     /locus_tag="L2X49_002834"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBN4797897.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGTDKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            11462..12964
                     /locus_tag="L2X49_002835"
     CDS             11462..12964
                     /locus_tag="L2X49_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBN4797898.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDLMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASAEQQNNPPTKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(13125..14207)
                     /gene="lptG"
                     /locus_tag="L2X49_002836"
     CDS             complement(13125..14207)
                     /gene="lptG"
                     /locus_tag="L2X49_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBN4797899.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(14207..15307)
                     /gene="lptF"
                     /locus_tag="L2X49_002837"
     CDS             complement(14207..15307)
                     /gene="lptF"
                     /locus_tag="L2X49_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBN4797900.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            15574..17085
                     /gene="pepA"
                     /locus_tag="L2X49_002838"
     CDS             15574..17085
                     /gene="pepA"
                     /locus_tag="L2X49_002838"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBN4797901.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            17343..17786
                     /gene="holC"
                     /locus_tag="L2X49_002839"
     CDS             17343..17786
                     /gene="holC"
                     /locus_tag="L2X49_002839"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBN4797902.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            17786..20641
                     /gene="valS"
                     /locus_tag="L2X49_002840"
     CDS             17786..20641
                     /gene="valS"
                     /locus_tag="L2X49_002840"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBN4797903.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(20695..21870)
                     /locus_tag="L2X49_002841"
     CDS             complement(20695..21870)
                     /locus_tag="L2X49_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBN4797904.1"
                     /translation="MDVPSHSFVFHGTGERYFLICVVNLLLTIITLGIYLPWALMKCK
                     RYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCVLTLSLLV
                     LLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKM
                     LHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTY
                     CIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQ
                     LIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGIT
                     GGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         5866 a         5404 c         5308 g         5302 t
ORIGIN      
        1 agactcaggg gcaaagattc tgtctcagta aatcaggctc ttaccgacaa attcctgagt
       61 ttaccgtcag aactcagaaa atcactgaca tgggacagag gaatggaact ggccagacat
      121 ctagaattta ctgtcagcac cggcgttaaa gtttacttct gcgatcctca gagtccttgg
      181 cagcggggaa caaatgagaa cacaaatggg ctaattcggc agtactttcc taaaaagaca
      241 tgtcttgccc aatatactca acatgaacta gatctggttg ctgctcagct aaacaacaga
      301 ccgagaaaga cactgaagtt caaaacaccg aaagagataa ttgaaagggg tgttgcattg
      361 acagattgaa tctacagtag ccttttttaa tatttcattc tccatttcaa tgcgttgtag
      421 ctttttcctc agcttacgta tttcgatttg ttctggtgtt atcggagagg cttttggtgt
      481 tttgccctga cgctcatcac gcagttgttt gacccatctt gtcattgtgg aaaggccaac
      541 atccatagct ttggcggcat ctgccaccgt gtatgtctgg tcaacaacca gttgagcgga
      601 ttcgcgttta aactctgcgc taaaatttct ttttttcatt ggagcacctg tgttgttctg
      661 aggtgagcat atcacctctg ttcaggtggc caaattcagt aaaccacttc accacttcag
      721 agcagacgag acaaaaagaa tggttgacgc atcagctcaa ccccatatag ttgtaacact
      781 tgagccaaac ccttgggccg ctttttactt tgatattaac attgctaata cagggaacgc
      841 acctgcctat aatgttgagg ttgtgtttga tcctccacta gtaaatgcgg agcatagaga
      901 aaaaagtgag attccgttta gtaaggtaag cgtgttaaaa aatgggcaat cacttaccag
      961 caatctctgt aagtatgaac aaatcaaaga tcaaatttat aatattaata taagctgggc
     1021 aagcaaacct aaatcaaacg atagagaaac aaatgaatat gtgtatgaca tggcgacatt
     1081 tgaaggaata agttatctag gagcgagaag cccattgacg caaattgcag aacaaattaa
     1141 aggtataaga gaggattgga aacctattgc acaaggagct aaaaaagtaa aagcagacgt
     1201 atatacttca agcgatagaa acgaagaacg cacgtatctg caagagcaac acgatttggc
     1261 aataaaaagg agagatgaga aaagagaaaa aagattagag tctggtgaat aattttaaag
     1321 ggagtgggta actaacccac tcgtaactat aaacctgtaa ttaatcactt atttttgaca
     1381 acagataatt actgaacgca ctgcaagtga ctaacataaa tttagcttca gctaaggtag
     1441 gatttacatc ttcttctgtt agcgcatgac gtattccacc ctgatcactt gtataaccat
     1501 agagctgact aaaagcgcct ttcattgcag agtgtatata tcctttttcc tctatagctt
     1561 taagacaagc ccccaaggtt cctttatcat tgcccgtgat tttcctgcat aaagattcaa
     1621 ttgcagagat agactcttta atcgagtttc tgtagtctgg ctgctctcta tccgtcatta
     1681 gttgtaacgc cctttcgaaa tggctacgcg atgaatcagt gccattatca actgcgttct
     1741 gaacactttc aatttcgtta tcatttgaaa taggagtaat acaaccattt attatggtat
     1801 aaccaacgcc atgcttttta aagatggaat tgagatgctt cgatagatta atatatgaat
     1861 tagttctctc aatgatgaac tcaattaaat catataccaa ataccatgct tccccatata
     1921 tataatctcg gatagcagtc agcaacgtct tatcactttt gtatccactt tcataacgag
     1981 gaatattatc cgcaggttga tttagataat atatccacac agactgcgca cattttgttg
     2041 ctgtagcagt ttgacgattg ttagtccaaa ggaaaagata taagcaattc cacaatgcca
     2101 tgcgtgtatc agaattaaga tcattcagct ggacatgctc tctaacgtca acatgaccat
     2161 acctcacaga aaatggcttt atcattcttc ccctgcctga ttgttatgtt cgtttaaatt
     2221 atgcagtcta agtttgatac aatcatatat taagcaatag aatccataaa gccccgttgg
     2281 tggtaagaca acaagcggag cctcttgcga cttcatcagg gttatgtcag ttagtgaggc
     2341 ttatcaggat gaccatgtac ccaaggggag cgaggccgaa ccgctggcag agcttgcata
     2401 tggtctgcaa gtaccgtgac caccataaac ctgtttatcc cctgcggcgt ctcgcgacgg
     2461 cgacagaatg ggggcatcgg gctaaaatgc gctgcactaa aagttttggg attgtttaga
     2521 gacggtggct acgtcatagc tttcaagttg agtcagtacg tgtttgacgc ttggaacgac
     2581 acgccgctat gagttaacat cccaattacc actatcaacc acagcggctt tttcaaggga
     2641 cataacagag catttcttac aggcaaaaga gacagattta atcgtctaac taactgtatt
     2701 gtaaggaatt tagtaacttg gtgccgaagg ccggactcga acctaagtct ctataacctg
     2761 ctgtaatcac gttcctgtta gctgttaaga gagcaatcgt aggacctttt tgttctaggg
     2821 gctacatatt aagtttgatc cgctggacgg aatcaaaccg agtgttcgtg gcggcataaa
     2881 acatcaacgt gccatacctg ccttttaatg tttctggagg tctgccgagt cgggaggaaa
     2941 gaacatttgt cagctatgtg gacgttgaat aaacgccact gcgttcggat tttgaaacca
     3001 gttagacccc ggcagacctt gttcagaata actatttaac tcaatgttat ttatatgaat
     3061 taagccatta agagccaaag cgaaacgcca ccgtaacgca cccgcatttt gctgttttat
     3121 tcactcttat agaaacaaag ggctgatgaa acataagact atcagcatct aatgaatact
     3181 gttcatccat acagcataaa aattcataaa cttacacttg agctactttc tcagtttgtc
     3241 ccaccgaacc gctgatacga taccccagtt gccgctagga ttaggcctac gccattttgc
     3301 tgtctccatg ctacgaagat actcaagagc ttcagcaaac gtgccaaaac gcttctgagc
     3361 cgctttatca cctacgccaa acgaaccatc acgggtgctc gccaaaccgg ggtgaaaaca
     3421 ggaaccatcg gctgcgtatg gaaccaacag ttccgagtcg ttatcttcag atgggccagg
     3481 cgctaacaac tgccacggct caacgctcag agcgttagcc agcacctcaa tcgcatccag
     3541 agacgcatta gagccaccac gttcaattct gctcatataa ctacgtgcaa acccgcaccg
     3601 atcggcaaag gcctcttggc tcaatccggt ggcaatgcgc agttctttta cccgctcgcc
     3661 aaactgtatt ctcagggatt ttttcatgca ctgaatgtgc aattttgacc actgtaaggc
     3721 cacgctccat atgagatatt ttgaatccaa tctcacttca gcgagcttag tcactagcat
     3781 tcatcggctg tatcatgaca atcctttttg attcaaaaca aacaccaatg gtgacattta
     3841 aaacccaaca agtatgatga tcatcatcgg ttgggcgtca ccagcgttcg ctatcaggcg
     3901 cgatgtggtt tggaaagtga gggaatcatg acgggagcag actttttatt accgattgca
     3961 gcgggagccg cgattagctg ggccagttat agtgtagttg cccatgctgt atcaaagcag
     4021 cacatcagca cgctacgcgc aactgtatgg ggtgctctgg gcactctctc attggtgctg
     4081 ggcgtatgcg ccttttctgc tgtaggtgct gagcatcaga cagcacgaaa agatatcgta
     4141 tgtgcagatg acaacccgga atgctatgcg aaaacgcacc ataacccggt cagggagtgc
     4201 aaaaaggtaa tggatgagaa agcgaagttt cgccatgtgt ggcaagaaag cgaagcgcct
     4261 gtatttaata cttatctctg gcacgatgag aacgcgaaga ttattcaggc atttgggcaa
     4321 caagctaagg tagttaacag cttggggatg ctagctccgc ttcaatactt ttgtgttttc
     4381 aacgcaaaaa caggagaagt cattgcggcc tctttcgaat aaaaatgtga gggaattcac
     4441 cctaacatca aaaataaact tatgtaatga cctcgcattt aattattcat ttaaagattc
     4501 aagaaatgtc cagcctttat gagtaacata atacatttgt ccttttgccc ctctttcaat
     4561 cacaaaatca aaggataaca gttcgttaag tgcgccctcc caacgggcta cctctctagc
     4621 cgaacttcca ccaaaggatt gatttcccgc ttgaatatcc gtgccaccta aatgccttat
     4681 cacataaatt gttccgctat catcgacaca tgctaatttc agcaaactga gtgcatcggc
     4741 ggacaaatta ctttctggca aagattgact tgtattcgca tcaggagaat tgttaatggt
     4801 agatataaga gaagacaaat aagggctact agaaataatt atcgaaagtt gcctacggac
     4861 tttttctttg aaatcagata agtcattaaa agactctatc aatccctttt gcatgcactc
     4921 agttttgaac aatttaagag actcatattg ttctctatca aggctttcag gggcaacagg
     4981 ctttgaggaa aagtaaatca ttgccggttt tcccgcattc aaatgacggt ggatttcttc
     5041 gactgttccg ctggcctcgt tcccagtcgg tgagcctaga cgagtccaaa acacaccgat
     5101 taataaatca caatctacta ataatctttg attaatcaac tcctgaggcc taactcctaa
     5161 ttcgggtgat gtatgagttt cccatccaat tggtgtcaac attacttttg aacgagctga
     5221 atttatatca ttccactcat ggatcatttc acgaatgact tgtctctctt cagcgacatc
     5281 accaggagaa gcaatcatta cagggataac agttgctgtg taagccataa actctccata
     5341 ttcaatcggt aatagctgct acagtcgctc ttgaaacgcc atattgacgc gctaacgcac
     5401 tgatactcaa accgctattc tttttctcta aaatctctct acgggcttct gacgcgattt
     5461 taggaggtct gcccaatttc ttaccttcgg cttttgctcg tgatagtccc gcctgagtgc
     5521 gctcaatcag cagatcacgc tccatctccg caaccgcggc gagcatagat aatagcaact
     5581 tcccagcggc agaagtgaga tctgtgttac caagctggtg gacaagaacc ttgatattac
     5641 ggtcagccag cgcccttacc gtttgcaaga cgtcaatcgc atcacgcccc agacgatcaa
     5701 gcttcgctac caccaacgtt tcaccatcgc ggattttatt gagcatttca gaaaatgctt
     5761 tacgttgcat tgccggaacc ttcccgctaa caacatcggc aaaccagtaa tcaatttgcc
     5821 acccagcctg cccaagctcc agccgttggt tctcagtatc ctgctgcgat gtcgaaacac
     5881 gtccgtagcc aaaagttgcc attgttatct ccccatctga cccctgccag aaaacgaggc
     5941 caacattagg atggcctgcc agaaaagcca aggctatttt tccaacagtg tgaaagcgtg
     6001 cgattgggag gtgccagaaa acggtcgttt gttggcgctt aaaacggata aagacatgtt
     6061 aggctgaaac catcacttca taaggacaac cgaaatgatt aaagacgcga tttttcaccg
     6121 tgcggtcaat atcgctactc gttatcacga gtctgggacg aatcaaaacc atatgccctt
     6181 ttcatagggc taaacccttc atacgccgat gctgagaagg atgatcgcac cttgagccgc
     6241 tgcatatcgt ttgccaaaag ctggggctac ggtggcgtat acatggccaa cctgttcgca
     6301 ttcgttcaca cccagcgaca tgaaatgatg aaagcgtctg atcccattgg caaagataac
     6361 gatagccact tgatacgtct tgttagcggt gccggactgg ttgtcgccgc atggggaaat
     6421 gaaggaagac acctcaaacg ctcaacgacc gtccgacagc tattgccaga gagcacaatg
     6481 tgcttcgtct taaacgctac aggtgagccg aagcaccctt tgtatatgaa aaacgacagt
     6541 gttctcatac ctttaggtta acatcgataa ctgcgttcag aatatgaacg cagccatatg
     6601 atatatatga cttttcttta aaatgatggt gtgctagctt ctctcctgac acctacctca
     6661 tgcggtaaac cggtccgggt aggtggagat caccgctgtt tagcggaaga cgtcaggatg
     6721 ggaactctgc gtaacgttga ttgtggtagg tagggcgcgc aagcgtttga tgcagccaag
     6781 cagagggtga aagtagctat cggcggattc ttgagtagct tcttttgcgc ataatgcatc
     6841 tttcaagaaa ggtcgtcaca ccacgaggaa gacttgatct tcatctggca aggggaaccg
     6901 agcaacctac aggctatctt ctcagatggc cttatggttg atatgtccgg cttccccccc
     6961 gaggaagggt ttacatctta aagaacaagt tatgttttaa catttttaac aattacactt
     7021 ctctgctgtt tggtgtctta tggcgttgcc gtcaaaagta aacctgccgt ttttacctct
     7081 cctgcgtaac aaccaaatac gaaccagaag ctgctaacgt tcaacgtgtt cgcggtgtaa
     7141 atgcagccag ataagttggg agcgaaaaag tggatgccct aggtttggaa gcgcccctct
     7201 caggcatagc gtccgaaaat ttccacggcg gcaggcttat ccttatagag ccaaacagac
     7261 tcaaaagtta agaagcgcgg taaagcttaa agctcgccca gatttctcat tgattgctcg
     7321 aactccgcta cgcttgtttc aactggcttc gccagtcact ctatgagagc gccgcgaatt
     7381 ctgcaccatg atttcgacta ccttctttaa aacactcgcc agagagcctt acagcgcgtt
     7441 tgaaccgcat atcgcagctt ttgcttacgg atgactgtct gattagcagt agcttcatgg
     7501 cccagtccgg tttactttat tgctatcaac aagggagccg tccagaggcg acgcggagat
     7561 aatgctttga gaaccgtcga gatactgcaa tacaccttac gcaaaggcag cggtgccgct
     7621 tttcacgcca ttatgcagga aatcagtgtt cccctacacc aacgccacgg gatcgatgtt
     7681 gtttcgtttg gtaactctct gcatgacccg gactgctatt acctgattcg cgcctttgtc
     7741 agccctaaaa gcatgactac ggttctcgac gctttttacg caagcgatga ctggcgcagc
     7801 ggcccgcgcg aagacatcat tggcaacata gaggccagca ttaaaacagt gataaatcta
     7861 ccatcagaaa gcgtagaagg gctgcgggtg caatcgtagg tcatcaagtt tcaccaaacg
     7921 tattttgagc gtctccaaat tcagcactat gaaatccacc acccttaata aaaaacgcgc
     7981 caggaagcgt cccagcgcat tcaagcatca ctcaactgaa tagtaactga ttcaacctgt
     8041 ttgtttgcca cgctttcttc gcaacgcatc tctgacagca tccagcccgt caggaatgtt
     8101 gaattccgct atatcaggta acgaaaaatc aattttctcc acgacttcaa tcatatgaat
     8161 tggtttgagg tctttgctat atcgcacttc cgtttcagtt tcgccgcgca catgcccaag
     8221 tagctgcgct cgtaattctg agctaacgcc cttctccttg agcaaagtcg aaactgagtg
     8281 gcgaaaagaa tggaaagatt tgcttctgtt cctctcgagt ttcaaccgcc ccgcaagcaa
     8341 cgattcattg aaccatgcgg aaactggcct accatagcct tttgttttat gtggtttaag
     8401 ctccgggaag agacgggtat accctgcatc ttttaaggcg ttcacgtagt cgatgaaacc
     8461 gagactaatc agctttgaat ggagcggaat cttgcggcga gcattagcat ttttgagttt
     8521 ctttccgctt tcattatcgc tttcaaggtt caaataatag acgccgtcgt ctgccagaac
     8581 atcactcagc agtaactgag cgatttcatt aactcgcgcc cccgtcatca agcccagcaa
     8641 aggtgcccaa tagttaaacg ggcaatattg atggaatcgc ccttgcgcat tgcgttcaac
     8701 agttccagcc tcaaaccacg gtaacgaaaa aatcgcttta atttcatgcg gttcaaatat
     8761 gtcagtgtga tcctgatctc gctcatcttt atcatcggat ggaaaaaagt tttcgcatgg
     8821 cgatttcagt agcttaccgt cagcaacagc ccacttcatt gcaccataaa tgccgttgat
     8881 atacttccta acggattcct cagtcatcaa tttatcgccg cattctgccg ctttggcgat
     8941 taactgatcc agcgttttca ctccatattt agctttgact aaattacggt ttgctgggat
     9001 cgtgcgcagt ttgctctcat aagcccggag gaaatcgcga tcaaaatctt ccaactgaat
     9061 gtcacccatc gcttcgatca taaagctgaa atagttacgg atatcactta tagccgactc
     9121 cgataccttg cccttgcgcc gatgctttag atattcagag cataaccacg acagattctt
     9181 agtgtaatac tttcgattaa catactcgtt atcgatagta ggggcaggag cggcaagagg
     9241 cacggtagga tgaacactag cgactggagg cgtggtctta gccagccaat caagccttag
     9301 gctttctgca tggactttat tgatcaaaag cgtatcgggt ttaattaccg gccaaccgga
     9361 ggtgaaaaac caaccgcctt tagcgtcgct tccggcagtt ttgaacaccg aagcttctga
     9421 ataaccgtcg ctaataatgt tacgtaaatg tgctggttgc gcagtgagtt gcagatagcc
     9481 tttagcaacc ccctcgatac ccttctcttc gccataatta agaacgtaac tgccatcaag
     9541 cgggtctagc tcagcctcag agagcgctgc aacataaaag ccagcctgcc ccgtcgccag
     9601 ccagaatacg ggcagattac ggttcatcac cgcctgtatt aattgcgcaa caggcaactc
     9661 aactgactga gcaaagttag aaagcgagat cataccctca ccagcaagca gcggggcgca
     9721 gtcacttaaa gttcgataat ccaattgttc atactcttgt aaggtctgat gccagaccgc
     9781 caacaggagc gcggacttct gctttgcttc atgaagatcg tgagtgcctg ttgaagtgtg
     9841 aatctcacac tgtcctgcgt atcgacgaaa acgttgggga atgctaatgc ggacaacata
     9901 aatgccgctg gttcgacgat agagtttata tcgctgagac atcgcctcgt ataccctttt
     9961 gtaagagggg ctacgcgatt tcaagcaaaa ttatggatta aattgtttta aaacaacgac
    10021 ttagataaga cttggtgccg aaggccggac tcgaaccggc acgtatttct acggttgatt
    10081 ttgaatcaac tgcgtctacc gatttcgcca cttcggcact gaagaggtat gcggaaaacg
    10141 ttgtggatta tacctgtcgc acgccaccat gcaagcgcca gcttgcaccc acctcgctaa
    10201 gtgctgaaat tttcagcatt acactcagcg atatgtaacc tttgtcacac tccaggcacc
    10261 ccgccctgcc atgctctaca cttcccaaac aacaccagag aaggacccaa aaatgtcgat
    10321 gataaaaagc tatgccgcaa aagaagcggg cggcgaactg gaagtttatg agtacgatcc
    10381 cggtgagctg aagccacaag atgttgaagt gcaggtggat tactgcggga tttgtcattc
    10441 cgatctgtcg atgatcgata acgaatgggg attttcacaa tatccgctgg ttgccgggca
    10501 tgaggtgatt gggcgcgtgg tggcactcgg gagcgccgcg caggataaag gtttgcaggt
    10561 cggtcagcgt gtcgggattg gctggacggc gcgtagctgt ggtcactgcg acacctgtat
    10621 tagcggtaat cagatcaact gcgagcaagg tgcggtgccg acgattatga atcgcggtgg
    10681 ctttgccgag aagttgcgtg cggactggca atgggtgatt ccactgccag aaaatattga
    10741 tatcgagtcc gccgggccgc tgttgtgcgg cggtatcacg gtctttaaac cactgttgat
    10801 gcaccatatc actgctacca gccgcgttgg ggtaattggt attggcgggc tggggcatat
    10861 cgctataaaa cttctgcacg caatgggatg cgaggtgacg gcctttagtt ctaatccggc
    10921 gaaagagcag gaagtactgg cgatgggtac cgataaagtg gtgaatagcc gcgatccgca
    10981 ggcactgaaa gccctgtcgg ggcagtttga tctcattatc aatactgtga acgtcagcct
    11041 cgactggcag ccttattttg aggcgctgac ctatggcggt aatttccata cggtcggtgc
    11101 ggttctcacg ccgctgtctg ttccggcctt tacgttaatt gcgggcgacc gcagcatctc
    11161 tggttctgct accggcacgc cttatgagct gcgtaagctg atgcgttttg ccgcccgcag
    11221 caaggttgcg ccgaccaccg aactgttccc gatgtcgaaa attaacgacg ccatccagca
    11281 tgtacgcgat ggtaaagcgc gctaccgcgt ggtcctgaaa gccgacttct gatccctttg
    11341 ccgggagcct gtcgcccggc acttaaatct ctttggaagg cgtctcgcaa tctgctgaat
    11401 ttagcggtga agagtgcaca gccgttgcct atactccagg caggaaactg gaggaaatct
    11461 catgagcgaa cccctgttaa ttgcccgcac gccggacaca gaactgtttt tactgccggg
    11521 aatggctaac cgtcacgggc tgattactgg cgcaacgggg acgggtaaaa ctgtcacgct
    11581 gcaaaaactg gcggagtctc tatcggaaat cggcgtgccg gtgtttatgg ccgatgtgaa
    11641 aggcgatctg accggtatcg cgcaggcagg aacggcgtcg gaaaaactgc tcgcaaggct
    11701 taaaaatatc ggcgtaaatg actggcaacc gcatgctaat ccggtggtgg tgtgggatat
    11761 ctttggcgag aaaggccatc cggtgcgggc gacggtttcg gatctggggc cgctgttgct
    11821 ggcgcggctg ttgaatctca acgatgtgca atctggcgtg ctgaatatca tcttccgaat
    11881 tgctgacgat caggggctgt tactgctcga ctttaaagat ctgcgggcga ttacccagta
    11941 catcggcgat aacgccaaat ctttccagaa tcagtacgga aatatcagta gcgcatcggt
    12001 tggtgccatc cagcgcgggc tgttgtcgct ggaacagcaa ggcgcagcac acttctttgg
    12061 tgagccgatg ctggatatca aagacttgat gcgcaccgat gccaacggta aaggcgttat
    12121 taatatcctc agcgccgaga agctttatca gatgccgaaa ctgtacgccg ccagcctgct
    12181 gtggatgatt tcagagttgt atgaacaatt gccggaagcg ggcgatctgg agaagccaaa
    12241 actggtgttt ttcttcgacg aagcacatct gctgtttaac gatgcaccgc aggtactgct
    12301 ggataagatt gagcaggtga taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt
    12361 ttcgcaaaac ccgtctgata ttccggataa tgtgctcggc cagctcggta atcgcgttca
    12421 gcacgctttg cgggctttta cgccaaaaga tcagaaagcg gtaaaagctg cggcgcaaac
    12481 catgcgggcc aatccggcgt ttgataccga aaaggcaatc caggaactgg ggaccggcga
    12541 ggcgttaatc tcgtttctcg atgcaaaagg aagtccttca gtggtggaac gagcgatggt
    12601 gatcgcgcct tgttcgcgaa tggggccagt gacggaagat gagcgtaatg gcttgattaa
    12661 tcactctccg gtgtatggca agtacgagga tgacgtggac cgggaatccg cctatgagat
    12721 gttgcaaaaa ggcgtacagg caagcgccga gcagcaaaat aatcccccca cgaaagggaa
    12781 agaggtggcg gtggatgacg gcattcttgg tggattaaag gatattttgt ttggcactac
    12841 cggaccacgc ggcgggaaga aagatggtgt ggtgcaaaca atggccaaaa gcgccgctcg
    12901 ccaggtgacg aatcagattg tgcgtggaat gttggggagt ttgctggggg ggagaaaaag
    12961 gtaagccagg tgcgatttgt ctttgcctga tgcgacgctg gcgcgtctta tcatgcctac
    13021 accactgcaa tatattggat tttcataatt tttcaggaat gaacgaagca cactttgcta
    13081 acaataataa agggagcttt cgctcccttt attcgttcat tcggttacga ttttctcatt
    13141 aacagccaca ggctgattaa gaagaagctg gcgcttggca acagtgcgcc gatgatcggc
    13201 gggatgccat aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
    13261 ccgaagctga taccggtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
    13321 atgaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
    13381 cacatgttga gctgataacg tccagcgtcc tgaccgctcg acttcagata cttcacgtag
    13441 ttgtgcaaac cgctgattga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
    13501 ggcgtgaggt tggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
    13561 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
    13621 ttcgcagtag cggcatagcg tacggattgc agacgacgat tctcgttaaa ggcataaatg
    13681 ctgatgccac ctaactcttc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
    13741 tctttcgccc ataagccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
    13801 cggtagttac gcgccatctg ctcgccctgc ggcgcgaccc attcaccaat cgccatcgtc
    13861 agcaagacta gcggaatggc ggttttcatc accgacagcg caacctgcat acgggtgaaa
    13921 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
    13981 gccccaagca gagctgccat cgggaagaag atttgcacat ctttcggcac gctcagcaag
    14041 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
    14101 acaaacttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
    14161 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
    14221 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
    14281 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagcgtcggg tccagcttac
    14341 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
    14401 tggctggcag catcgacagt acgcgtccct gacgtgggtt aaccacgctc agcggtacga
    14461 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
    14521 gggcacgatc ggtgtcagtg ttccacaatg tgcgcatatc catctggtcg gtatcgttcg
    14581 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
    14641 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
    14701 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
    14761 cattaccttt tggtcgaatt tgcgcgagga acacatcttt gaaatcgctg ccgtcaacgc
    14821 tttcgatgaa cagcaccgaa ctgccattag tcgcttgctg gaattgccct tgcgccagcg
    14881 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
    14941 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
    15001 ttgccgcttt caccagaacc gctttgctca ggccgcaggc atgcattacc gtaatttcac
    15061 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
    15121 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
    15181 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
    15241 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
    15301 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
    15361 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
    15421 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
    15481 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
    15541 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
    15601 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
    15661 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgtcgg
    15721 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
    15781 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
    15841 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcaat ggaagcggtc
    15901 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
    15961 gtcgagacag caaaagaaac gctctacagt ttcgatcagc tgaaaacgaa caagagcgaa
    16021 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
    16081 ggtgagcgcg cgatccagca cggtctggcg attgccgccg ggattaaagc agcgaaagat
    16141 ctcggcaata tgccgccgaa tatctgtaac gccgcttacc tcgcttcaca agcgcgccag
    16201 ctggctgaca gctacagcaa gaatgttatc acccgcgtta tcggcgaaca gcagatgaaa
    16261 gagctgggga tgcattccta tctggcggtc ggtcagggtt cgcaaaacga atcgctgatg
    16321 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
    16381 aaaggtttaa ccttcgactc cggcggtatc tcgatcaagc cttcagaagg catggatgag
    16441 atgaagtacg atatgtgcgg tgcggcagcg gtttacggcg tgatgcgtat ggtcgcggag
    16501 ctacaactgc cgattaacgt tatcggcgtg ttggcaggct gcgaaaacat gcctggcggg
    16561 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
    16621 aatactgacg ctgaaggccg cctggtactg tgcgacgtgt taacttacgt tgagcgtttt
    16681 gagccggaag cggtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
    16741 catcatatta ctggtctgat ggcgaaccat aatccgctgg cccatgaact gattgccgcg
    16801 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacaa
    16861 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
    16921 gcaggttgct tcctgtcacg ctttacccgt aagtacaact gggcgcacct ggatattgca
    16981 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
    17041 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaattgcg tcaggcaagg
    17101 ctgttattgc cggatgcggc atgaacgcct tatccgacct acacagcacc gaactcgtaa
    17161 gcctgataag acgcggcagc gtcgcatcag gcaatgctgt tattgtcgga tgcggcgtga
    17221 gcgccttatc cgacctacac acagcactga actcgtaggc ctgataagac acaacagcgt
    17281 cgcatcaggc gctgcggtgt atacctgatg cgtatttaaa tccaccacaa gaagccccat
    17341 ttatgaaaaa cgcgacgttc taccttctgg acaatgacac caccgtcgat ggcttaagcg
    17401 ccgttgagca gctggtgtgt gaaattgccg cagaacgttg gcgcagcggt aagcgcgtgc
    17461 tcatcgcctg tgaagatgaa aagcaggctt accggctgga tgaagccctg tgggcgcgtc
    17521 cggcagaaag ctttgttccg cataatttag cgggagaagg accgcgcggc ggtgcgccgg
    17581 tggagatcgc ctggccgcaa aaacgcagca gcagcccgcg cgatatattg attagtctgc
    17641 gaacaagctt tgcagatttt gccaccgctt tcacagaagt ggtagacttc gttccttatg
    17701 aagattctct gaaacaactg gcgcgcgaac gctataaagc ctaccgcgtg gctggtttca
    17761 acctgaatac ggcaacctgg aaataatgga aaagacatac aacccacaag atatcgaaca
    17821 gccgctttac gagcactggg aaaagcaggg ctactttaag cctaatggcg atgaaagcca
    17881 ggaaagtttc tgcatcatga tcccgccgcc gaacgtcacc ggcagtttgc atatgggtca
    17941 cgccttccag caaaccatca tggataccat gatccgctat cagcgcatgc agggcaaaaa
    18001 caccctgtgg caggtcggta ctgaccacgc cgggatcgct acccagatgg tcgttgagcg
    18061 caagattgcc gcagaagaag gtaaaacccg tcacgactac ggccgcgaag ctttcatcga
    18121 caaaatttgg gaatggaaag cggaatccgg cggcacgatt acccgtcaga tgcgccgtct
    18181 cggcaactcc gtcgactggg agcgtgaacg cttcaccatg gacgaaggcc tgtccaatgc
    18241 ggtgaaagaa gttttcgttc gtctgtataa agaagacctg atttaccgtg gcaaacgcct
    18301 ggtaaactgg gatccgaaac tgcgcaccgc tatctctgac ctggaagtcg aaaaccgcga
    18361 atcgaaaggt tcgatgtggc acatccgcta tccgctggct gacggtgcga aaaccgcaga
    18421 cggtaaagat tatctggtgg tcgcgactac ccgtccagaa accctgctgg gcgatactgg
    18481 cgtagccgtt aacccggaag atccgcgtta caaagatctg attggcaaat atgtcattct
    18541 gccgctggtt aaccgtcgta ttccgatcgt tggcgacgaa cacgccgaca tggaaaaagg
    18601 caccggctgc gtgaaaatca ctccggcgca cgactttaac gactatgaag tgggtaaacg
    18661 tcacgccctg ccgatgatca acatcctgac ctttgacggc gatatccgtg aaagcgccca
    18721 ggtgttcgat accaaaggta acgaatctga cgtttattcc agcgaaatcc ctgcagagtt
    18781 ccagaaactg gagcgttttg ctgcacgtaa agcagtcgtt gccgcagttg acgcgcttgg
    18841 cctgctggaa gaaattaaac cgcacgacct gaccgttcct tacggcgacc gtggcggcgt
    18901 agttatcgaa ccaatgctga ccgaccagtg gtacgtgcgt gccgatgtcc tggcgaaacc
    18961 ggcggttgaa gcggttgaga acggcgacat tcagttcgta ccgaagcagt acgaaaacat
    19021 gtacttctcc tggatgcgcg atattcagga ctggtgtatc tctcgtcagt tgtggtgggg
    19081 tcaccgtatc ccggcatggt atgacgaagc gggtaacgtt tatgttggcc gcaacgaaga
    19141 cgaagtgcgt aaagaaaata acctcggtgc tgatgttgtc ctgcgtcagg acgaagacgt
    19201 tctcgatacc tggttctctt ctgcgctgtg gaccttctct acccttggct ggccggaaaa
    19261 taccgacgcc ctgcgtcagt tccacccaac cagcgtgatg gtatctggtt tcgacatcat
    19321 cttcttctgg attgcccgca tgatcatgat gaccatgcac ttcatcaaag atgaaaatgg
    19381 caaaccgcag gtgccgttcc acactgttta catgaccggt ctgattcgtg acgacgaagg
    19441 ccagaagatg tccaaatcca agggtaacgt tatcgacccg ctggatatgg ttgacggtat
    19501 ttcgctgcca gaactgctgg aaaaacgtac cggcaatatg atgcagccgc agctggcgga
    19561 caaaatccgt aagcgcaccg agaaacagtt cccgaacggc attgagccgc acggtaccga
    19621 tgccctgcgc ttcaccctgg cggcgctggc gtctaccggt cgtgacatca actgggatat
    19681 gaagcgcctg gaaggttacc gtaacttctg taacaagctg tggaacgcca gccgctttgt
    19741 gctgatgaac acagaaggtc aggattgcgg cttcaacggc ggcgaaatga cgctgtcgct
    19801 ggcggaccgc tggattctgg cggagttcaa ccagaccatc aaagcgtacc gcgaagcgct
    19861 ggacagcttc cgcttcgata tcgccgcagg cattctgtat gagttcacct ggaaccagtt
    19921 ctgtgactgg tatctcgagc tgaccaagcc ggtaatgaac ggtggcaccg aagcagaact
    19981 gcgcggtact cgccatacgc tggtgactgt actggaaggt ctgctgcgcc tcgcgcatcc
    20041 gatcattccg ttcatcaccg aaaccatctg gcagcgtgtg aaagtacttt gcggtatcac
    20101 tgccgacacc atcatgctgc aaccgttccc gcagtatgat gcatctcagg ttgatgaagc
    20161 ggcgctggcc gacaccgagt ggctgaagca ggcgatcgtt gcggtacgta acatccgtgc
    20221 agaaatgaac atcgcgccgg gcaaaccgct ggagctgctg ctgcgtggtt gcagcgcgga
    20281 tgcagaacgt cgcgtaaatg aaaaccgtgg cttcctgcaa accctggcgc gtctggaaag
    20341 catcaccgtg ctgcctgccg atgacaaagg tccggtttcc gttacgaaga ttatcgacgg
    20401 cgcagagctg ctgatcccga tggctggcct catcaacaaa gaagatgagc tggcgcgtct
    20461 ggcgaaagaa gtggcgaaga tcgaaggcga aatcagccgc atcgagaaca agctggcgaa
    20521 tgaaggcttt gtcgcccgcg caccggaagc ggtcatcgcg aaagagcgtg agaagctgga
    20581 aggctatgcg gaagcgaaag cgaagctgat tgaacagcag gctgttatcg ccgcgctgta
    20641 atcaacaccg tgatgaaaac gaaggccgta gcatgctccg gcctttttta tctcttacag
    20701 aaatggtaaa gaaggcataa ttccccgtga aatactggct aagaagcctt tatctggttg
    20761 ttcttcttta ttgattaaag gaaggtcatc caaatcgccc agcaaatacg tattttttgc
    20821 ctgccagtca atcatccata ttttcagcag tggataagcc agaccgcccg taatcccgga
    20881 tatcaccacc aacgcacaca tgcgataaag cataccgtgg tatgttaaag ttgagcgaaa
    20941 acgaatacgc ccatcattca gtgacaggtt gctcataaaa tgatttcgca acgacaccgt
    21001 taaataactg gtgctaacag caatcccaaa ataatagatt aactgcgcga ttatcatttt
    21061 acgttgcatt tccataaatt gctgtaaatt ctcaatatca tcatttgcat acacagaact
    21121 atcatacgca tttaatattt gatcgaagat aatataacca gcaacagcga gaaaaggtaa
    21181 taaagctaaa aatgcgagaa tggcatattt tatacaatac gtagtatcta atttcacctt
    21241 gaaacgatgt ataccgaaac tggtattgct ccagagaaaa ctcattacca gactatataa
    21301 agtaccatta aaaataccaa tggaaacaat accaactatt gccatcagaa ccacagatat
    21361 aataacacta cttgaactat tggcatgtag catctttgtc gagataaaga aaacagtccc
    21421 catcccaatg gccattaaaa tcggcaagaa aaaggttacc caccagaacc ctttcaaaga
    21481 gcaattaaaa ctaaatctta cgccgttgag actagtcatc aaggcctgat aacgcagtcc
    21541 ttttgctgcc ataaatataa gcaaaaccaa cagtgacaaa gtcaaaacac agccaacaag
    21601 cggcatatct gctgacactg tcattaagat tgcgaaatag aaaaaaacaa aaacaagaca
    21661 actaacaaaa acattcccac cggtaatccc ataagaaaat cgttgtccgt taacttccat
    21721 attagcatag agataacgct tacatttcat taatgcccat ggtaaataga tacctagcgt
    21781 tataatcgtt aacaacaaat tcaccacaca aataagaaaa tatctctcac ctgtaccatg
    21841 aaaaacaaac gaatgggacg gaacatccat ttcattaata
//
LOCUS       DAFNBJ010000081        21150 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000081 DAFNBJ010000000
VERSION     DAFNBJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.081"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            37..3804
                     /locus_tag="L2X49_002842"
     CDS             37..3804
                     /locus_tag="L2X49_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBN4797905.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADNSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYINKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILIDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEMR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESLASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAATTASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDVPSQPDTGHVQADESELQATTDTVE
                     PAVRDDPTPQQPVQSTSSSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSPETTTTT
                     PAPDSIVYAPERPDAPISLNLDEIIKSLEGEERADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATDFYGGVIELTGSDAFKQKAMQLIIEHNIKVRMKFPDQRAA
                     LEKLRKEMAVGKDTVVTHKPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STVPASSPAEPGKTADAAQGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            3822..4169
                     /locus_tag="L2X49_002843"
     CDS             3822..4169
                     /locus_tag="L2X49_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBN4797906.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            4166..4858
                     /locus_tag="L2X49_002844"
     CDS             4166..4858
                     /locus_tag="L2X49_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HBN4797907.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFVGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            4869..5852
                     /gene="traN"
                     /locus_tag="L2X49_002845"
     CDS             4869..5852
                     /gene="traN"
                     /locus_tag="L2X49_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HBN4797908.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNT
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            5855..7144
                     /gene="traO"
                     /locus_tag="L2X49_002846"
     CDS             5855..7144
                     /gene="traO"
                     /locus_tag="L2X49_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HBN4797909.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGGGYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            7144..7848
                     /gene="traP"
                     /locus_tag="L2X49_002847"
     CDS             7144..7848
                     /gene="traP"
                     /locus_tag="L2X49_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HBN4797910.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAIVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            7848..8375
                     /gene="traQ"
                     /locus_tag="L2X49_002848"
     CDS             7848..8375
                     /gene="traQ"
                     /locus_tag="L2X49_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HBN4797911.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            8426..8830
                     /gene="traR"
                     /locus_tag="L2X49_002849"
     CDS             8426..8830
                     /gene="traR"
                     /locus_tag="L2X49_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HBN4797912.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVILFLGGLIGLKKVGKQGGMGLASC
                     IVSIVIGAVLVAGPEMMSRSQKQLGISSISIG"
     gene            8894..9082
                     /gene="traS"
                     /locus_tag="L2X49_002850"
     CDS             8894..9082
                     /gene="traS"
                     /locus_tag="L2X49_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HBN4797913.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            9066..9866
                     /gene="traT"
                     /locus_tag="L2X49_002851"
     CDS             9066..9866
                     /gene="traT"
                     /locus_tag="L2X49_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HBN4797914.1"
                     /translation="MQHSIKNLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLVKLIRRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTEPLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            9956..13000
                     /gene="traU"
                     /locus_tag="L2X49_002852"
     CDS             9956..13000
                     /gene="traU"
                     /locus_tag="L2X49_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HBN4797915.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWALSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            13000..13614
                     /gene="traV"
                     /locus_tag="L2X49_002853"
     CDS             13000..13614
                     /gene="traV"
                     /locus_tag="L2X49_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HBN4797916.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            13581..14783
                     /gene="traW"
                     /locus_tag="L2X49_002854"
     CDS             13581..14783
                     /gene="traW"
                     /locus_tag="L2X49_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HBN4797917.1"
                     /translation="MQRKTLLAALIAILNGTAYQAHAYSVTVVASRPVEQQVVPRMEA
                     IKGVLSDILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            14812..15396
                     /gene="traX"
                     /locus_tag="L2X49_002855"
     CDS             14812..15396
                     /gene="traX"
                     /locus_tag="L2X49_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HBN4797918.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRRIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            15493..17661
                     /locus_tag="L2X49_002856"
     CDS             15493..17661
                     /locus_tag="L2X49_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HBN4797919.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSDPTLIGSMFWVFNNIIAVLAIVWCIFISIRHVVRSGHQGQVFSV
                     GRDIVGILSFVAGFLMIVPTGNGWSLAQLIMLWGASIMGVGSANVMVQVAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTAKSSTSLMTESAKTASGNYT
                     VTISNGSGICGTASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQKAADEYERAVQKSLPTDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYK
                     TQLQNTTYTPPLGTVTSANEQRMANAQDPKSALSEMMSFFVYRTNWIATEISGTGTTS
                     DQVNPLIKMKNIGDYTLGTTEILWSGYTTVRVLATVGDNSIFGKVANLFSGLPKGLVA
                     LLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAVGTILNALFGAALVN
                     VQFNSLTGIFSLAGFLLIYARICTTTVAAIFALQAYLPDHVINFLGGREGANTLGSMA
                     NSVKEIFIGGSRNIRHTPGMKTDRLKNNTNGSDDKDGIKG"
     gene            17726..18388
                     /gene="excA"
                     /locus_tag="L2X49_002857"
     CDS             17726..18388
                     /gene="excA"
                     /locus_tag="L2X49_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HBN4797920.1"
                     /translation="MKAKKETPDRFPTWWLLYYVLRKAYFFLGIPFFLFCALTSTLML
                     FSSRYYGDNIEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEK
                     GYEGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKTLEIHTK
                     YINLPMVPIPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGVPVAE
                     VF"
     gene            complement(18460..18669)
                     /locus_tag="L2X49_002858"
     CDS             complement(18460..18669)
                     /locus_tag="L2X49_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBN4797921.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDITLAAIYALGEGRCQADNIIRE
                     LERLSQSDDMEIKIAAIKALGRIYR"
     gene            19001..19177
                     /locus_tag="L2X49_002859"
     CDS             19001..19177
                     /locus_tag="L2X49_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797922.1"
                     /translation="MNLVDAFVKKVISGPYEEYGKWWIDVEYISWGVSGKTRLMFDSK
                     EQALEVKEGYKFLT"
     gene            19836..20087
                     /locus_tag="L2X49_002860"
     CDS             19836..20087
                     /locus_tag="L2X49_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797923.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(20159..20311)
                     /locus_tag="L2X49_002861"
     CDS             complement(20159..20311)
                     /locus_tag="L2X49_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBN4797924.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            20584..>21150
                     /locus_tag="L2X49_002862"
     CDS             20584..>21150
                     /locus_tag="L2X49_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797925.1"
                     /translation="MRSRYVDRIIYMKKLLIRLIPDAIYEALEKTALHSERSLEAQAR
                     YILSCSVDNEKQLTGGERYQREITARLNQALSEANEVITAINLVPARIAEQLGHHDAI
                     ESENWFTGNAVPSFTELDELSDIFGCSPDWLKFGENVPYPKSSKGRINWNRGGEKDID
                     ALLEPDNKGRKVSSIHIFRVNESGNILIL"
BASE COUNT         5825 a         5376 c         5140 g         4809 t
ORIGIN      
        1 gtggtttttt tccgtctttt ttcaggagaa gcactcatgc cgtctgtatc ccgctaccgc
       61 acctggcttg ccgtgcctgc agatgaaata gaagatctca aaaaagcaca tcccccgatg
      121 aacgggcaca cgcccgtcat ctgggacaag gagcacaaac tctggttcgc ccgcccgggg
      181 gcggacctgt ccaggctgga ccgctggctg ccccggccac aggacgtctc catgaacggc
      241 agtgatccgg tcaccgagtt tgcccaggtt ctggagaatg ccggactggt tctgaaagaa
      301 ctgccggtca tggacgggaa aatccaccgt gtcccgaccg ccgatgacaa aaaaggacaa
      361 aaaagtggcg catatcgcgg attcctggac ggcagacctg ccggctggta cagggattat
      421 cgcagcgcgg ataattcgcc gatcacctgg accttctccg gcggagaaca gacagatccc
      481 cgcgcaagac tccacctgaa agcgcattca atgcaacgca gggaggacgc ggagcgggaa
      541 ctgaaagcac agtacaaccg acaggccgcg tatgcccgcc gctatatcaa caaatggccg
      601 caggccacag cacatgaata cctgacccgg aaaggcattc aggctgcacc gggggtgcgg
      661 gtaaacaaca aaaatgaact ggttattccc ttcagtaacc gtaacggcgc gatacgctct
      721 taccagcgta ttccggtcac cggggggaaa gatgcccgca tcctgataga ttcggagaaa
      781 accggtaact ggtttgcgct gggcacaccg cgcaacggtc agccggtact gtttgccgag
      841 ggctatgcca ctgcggcctc cctgcacgaa gccacgggcc ttccggtact gatgacggtg
      901 gatggaggca acatgattgc cgttgccgaa aatgcccggc aaaagtggac gcaaagcccc
      961 tttatcttct gtgccgacaa cgatcatgca atccgggtta acaaaggtat cgtcagtgca
     1021 acaaaagccg cagaactgac aggaggatcg gtcatctttc ctgcgttcac cgatgccgaa
     1081 aaagcgcagg ggctgaccga tttcaatgac ctggacgcaa gccggggacg ggccgctttt
     1141 cagcatgtca tcaacgccca gttagaacac atcggggttt ccacccccaa cagtaacacc
     1201 ccggaaatgc gcgaggcact ggtgataggc aacctcgttt ttacaccggt ccacactgag
     1261 gaaaaaacca tgactccgac agaatatcct gaaacatcac ccgatacagg gcacagccat
     1321 gaccagggac cttctctgcc gtccgccacg cagcaggaac aaccagctgc ttccagcagt
     1381 aacattgctg atgaaacaca gtcatttacc agtcacgcca ctgaaaataa cggcaaagat
     1441 gagcggcacg ctgataacgt tcaggcgcaa acggaaagct ctgtagttca ggactcccct
     1501 gcagaatcac tggcgtcccc ggaaagtaca acggcgtctg cccctgatga acccgctcat
     1561 ccggcggaac ctccggaaaa agttgtctcg gtggccacag ataaagactg gcgtgaattt
     1621 gaagctgaac tgtcacaacc ggaaaaaaac gagtcacaac aggaatccgg cacttcacct
     1681 gaaataccgg cccctgctgc caccaccgca tcccctgaag acagtccctc atctgaaacc
     1741 atgaatgaat cagccgagcc atcaccttcc cctgatgcag tcaaagagga aatgaccgtg
     1801 atgacggagc ctgatgacaa acaaagcacc ggaatatccc cggatactcc ccgggaagag
     1861 gcggtcaacc atgtaactga agacattccc cttcagccag agccgttcct tcctgagtca
     1921 gatggccccg aagaatacga aaactacagc gcatatcagg agctgatgaa caatgaaaat
     1981 cctgagcatc tgcaggaaaa cagtgatgtg ccgtcgcaac cggacacagg ccacgttcag
     2041 gctgatgagt cagaacttca ggccacaaca gatacagtag aaccggctgt ccgggatgac
     2101 cccactccac agcaacctgt tcagagcaca tcatcttctg ataatacttc atcgtttctc
     2161 gacaaagcgc gtggcttttt tacgcgtaaa aaaacggatt cacaggcgca tgagaacagt
     2221 gatcccacac cctcaccgga aaccacgacg accacaccgg cacctgacag tattgtctac
     2281 gccccggaac gccctgatgc accgatatcg ctcaatctgg acgaaatcat taaatcgctg
     2341 gaaggtgaag aacgagcaga cagaaccgtc ctgtacaaac tggatggcaa acctgccttc
     2401 attgaccggg taaaccggct ggaaatggtc aacggtgcca gcaatgacga tcgcagtgtt
     2461 ctggctgcac ttgctgtggc cactgatttt tatggtggtg tcattgagct gacgggcagt
     2521 gatgccttta agcaaaaagc gatgcagttg atcatcgagc acaacatcaa agtgcgcatg
     2581 aaattcccgg atcagcgagc ggcgctcgaa aaattacgca aggaaatggc tgtcggtaag
     2641 gatactgttg tcacccataa gccaacaccg gaacttaacc ggaatacccc tgaacaacca
     2701 gcagtcccag accctgttca ggaaaaagaa gccacacaat ccccggcgtc cccagtagcc
     2761 cctgaagcat caaccgtcag caccgtccct gcatcatcac cggctgaacc tggcaaaacg
     2821 gcagatgcgg cacaaggtga agaaccacca ggaaaacttc gcccgggaga gagcgtgact
     2881 gccgtactgc acaattttgg tcgtgctgaa tatgcacccg gcaaaggaga gagtttcttt
     2941 gttgagctta aaaaccgcag tggcagcaaa ctctactggg gggaacaact ggaaagtctg
     3001 gtcaaaaacc atcagaaagg tgacgtggtg acactgacac ttcagaaccg cgaacaattc
     3061 atcctcccgg gagagcaaaa agcacgtttc cggaataagt ggtccatgga gtcagttacc
     3121 aacgggatct cggtatcaca tgataatccg gataaaggtc agcgtattca ggcgattccg
     3181 gtcgaaacct ttatgaaagt ggctgcgcaa atcagccagg ggtggcccga agagatgaaa
     3241 gcgttacgta tgccagaaaa cgtcggctca catctgttta tcggtgaaga ccgacatccg
     3301 gtctccgcgc cacagaacgc aaatcaggta acagaaataa ccagcgcagc ccctgacaag
     3361 ctgacacctg ttctgggatc agtggataag gatacgcgtg aactcaacct gctacttgtc
     3421 cagagtgccg atgaacatct gcagggtgtg gtccggctga acggcacatt gtatccggca
     3481 ctggccaccc catcagccga caacagtcag ctggttatca atgcgctgac cgacaaaggt
     3541 ttacgctttg caggttatgg tgaagcggta aaccacgatg ctgacagcac gaatcgccct
     3601 gcaccggagc ttatgcagtt tcatctgaaa acccgggaag aaccgctttt tgctgctgtc
     3661 tacacgccgg aaaaacaacc tgatgcgttg taccggaacc tgggcttcga gcagtcatgg
     3721 cagcagtggt ccaacagcca aaagccggag gacaggcagg aaaaaactct gcatcaggat
     3781 ctctcccaca gccccggccg atgagccggt aaggaagcca tatgaacatg cataagtctc
     3841 tggttgccgt gctcggcctt atcctgattt catccacggc aagtgctgca acctgtcgtg
     3901 cgggtagcgc ggcacaggcc ggtagtaata caggatatga gcgagcacgt gcagctgcgg
     3961 atgcatggtc acaacgtgaa aacgatgttt cctcatccct gcagtcctgt ctgtcacgaa
     4021 tcaaaaaaat cagcataaac ctcccccagt tccccagcct tgacgacatt ctgtcgcagc
     4081 tggaaaacca ggtctgcgac gcggtcgtgg acaaggtgaa tgaaaagctg ccggggaata
     4141 ttgatccctg gaaggactac aacctatgac cgaaaatacc gagccatcct cgccatcaca
     4201 tccggcacct gacccggaaa tactgaagag cacaatcagg gctatgaagc agtcagaaca
     4261 gcgggcaaat aatgtccctg cgctgataaa agcgctttta tgtacgggca catgtcttct
     4321 tatcagtatt actggtaacg ccattcagta ctggcactca acgaatgtgg agcgcgaata
     4381 tttcgctaca gacaatggtc gtctggtcag gctggcacca acaagtcagc cagcatggag
     4441 tcagaatgat gcaatggcct ttggcagcca ggcactggcg accgcattca acctggattt
     4501 tgtgcactat cgttcacaga tctcctccct gtctccgcgt ttttctgacg aaggttttgt
     4561 cggatatgtg aatgcattac aggcatctaa cattctggag acgatcaaga aagagaagat
     4621 gaatctgacc gccacgacag gtgctggcgt tctggtacga cagggacaaa tgtccgacgg
     4681 cgtatggttc tggacattcc agtatcctgt ccgtatgcgt ctggtcggcc agacaaccag
     4741 caagccagaa cagtcttttg ttttcgaaat caccatccag cgtgttgacc ccagactgaa
     4801 accatccggt atggaaatcc ggcagatgat ctcccgtaac gccggaccga actcctgaga
     4861 taagtaatat gcaatcacgt tatttactga gcacactgct cctggtgtgc agcgccgcca
     4921 cgtctgcaga taacgcagga tggcagaacg cacgcacacc acaaacgaca aattctgcca
     4981 gcgatcacgt tcagaacacc tcccaaaaca ccggaggtgt tccggcaaca ctggtaaaag
     5041 gtgagctgcc tgcccccggg caggccagcc cgctggtaca ggatgcggca aggcttgatt
     5101 ctgagctctc cgccgatgag attcgctcac tacgcagtct catggcggat aacgagcggg
     5161 ccatcaatgc gcccatcacc agtgttgtac cccgtatcag ctctctgaca gttaatctgt
     5221 cccccggtgc cagccttcct cttgttcgca cagccatgaa caacctgagc gtggtgacat
     5281 ttaccgatat caacggttca ccatggccac aatccgatcc tccttataac gcggcaccaa
     5341 aactctttga tgtgcagtac aacgagaaca tggtgacaat cacaccattg cggccatggg
     5401 catccggaaa tatttctgtc tacctgaaag gtctgtcggt accggtcatc ctgaacgtaa
     5461 ccagtggcga aacagatacg ccgtcatcca gtcaggaaat ggacagtcgt ctggatctgc
     5521 gaataccacg gcagggacca accagcccag ttgtctccat cccgacggat aagatcgccc
     5581 tgcatgatgc aacattacag gcatttctgg atggtattcc tccccgggac ccttcagtta
     5641 agaggctgaa attcacaggt aatgtcccgg atacaacgat ctggcaacac ggcgatgacc
     5701 ttctggttcg ttcccgggcg atgttgcgcg atgaatttga acagaccttg tcctctgccg
     5761 acggaactca tctctggaaa cttcccgtca ccccactact gactttctct gtgaatggcc
     5821 agtccgttca cgtgacacca gaactggagt aatcatgagt gctgaacagg atgccggtaa
     5881 atccggaaag aaactggctg ccctgcttgg actgggcagc atcatcttat ttggcggcgg
     5941 ctatatcgcg ttttccaaac tatcagggag taacagcgat atgcagtcag ccgtgaacat
     6001 taacagtgca gcaagcggcg gaacgcgcag cgttaccgaa acgcctcact atcgtgaact
     6061 gttaagagcg gataatgaac gaggggccgc agctgcagcc cgcaataatc agacgttcat
     6121 tgccagtctt ccacaggggc ttgatattcc ggacactccg gaaaagcaac aacagcctgc
     6181 agcaaaaccg gaaaactatg ctcatcgcca ggcttccggc acaccacagg aggatcgagc
     6241 agccagtgaa aaacgtatgg agcggctgca aaagctgatt gtaagaataa aagatcagca
     6301 tcctgccggt agtacaccaa ccatagcaac gactatgtgg aataaatctc cggcagaaac
     6361 aacagggcag aatggtacgc agcaattcgc attacagaat gccagcctgt ccacgcctgt
     6421 agcagaaaaa gggatacaac tgatacctgc tctgacccgt ataccggcat atattgatac
     6481 tgcagtggat tctgataacc cgtcatccaa ggttatcgca accattcccg ctggcccctg
     6541 ggcaggggca acgctgttca gtccgggagt caaactggtg ggtaacggcg tggagatcca
     6601 tttcgaccgg atgagctgga acggcatgga ccttaaggtc aatgcatatg ctcaacggga
     6661 agacaacctg atgtcatccg tggccagcaa tgtgaataca cgctggttta aacatatcat
     6721 tctgccgtca gtgttaggag gtgtgggcag tattggcaca ctttataaag atgccaacac
     6781 gcaggttatt cagggtaact atggcacggt taccggacgc gtgggaatgc ccagtggtga
     6841 agctgttgcg ggcgttattg ccggtggtat ggcggaaaga ggaagtcaga tattaacccg
     6901 ccaggcagaa agtgagccct ataaacaggt agaagtgtat cagcatgaag tcgtatcaat
     6961 tctgtttgtt gacccggtaa tgacaaacga cgccaggtca tcttcccttt ccagtggcat
     7021 ttccccttca gttaatcgta catcacaggc ggaacaacgc tcgcaggcac gaatgcagac
     7081 agccatggag caaagaaaag ctgtgatgca acgtcggtat gatgaacaac cggagacacc
     7141 ataatgaaac ctgaaacaga aatagatgaa accggcagct tcggtgaacc cgaagagaaa
     7201 cccgccccct tctggaaacg ttccgtctgg gggatatctg tagcaacatg ggggctatgt
     7261 gcaatcgtgt tgctcgcagc catatggtat ctgttcctga gagcgccatc ggaaacaggc
     7321 atgcctccgt ttaatgatgc ggatgctggc gtacagacct ggcaaaccac acaagaatca
     7381 tcaccttccg ttcagtccac tgagactatg actgtccgga caggagaaga tatgagccag
     7441 cttgcccggg atgtaaaaac agagctggat aaccgcgatg aaaaaattca ggcaacactg
     7501 aatatgcttc acgacagcat taacaagctg ggcgaagcta taaaaaagga tgaagaatac
     7561 gcacaggaaa cacgccgtca gctggatgat atccggagcc gcctgaacgg aataatgaca
     7621 cagaaaagcg tcactgaatc ctcttccaca ccgcatccag caaagaaaaa aacgtcgtct
     7681 gtacttaacg gaatgaaaat catgtcgatg gaaactggta tggcctggat tcgctggcag
     7741 ggtagtacct gggctgtcag agaaggtcag acgctgggca atgtggtgat ccagcggatt
     7801 gatccgacta cccggaccat catcaccagt gcagggacgt tacgctgatg aacatggatg
     7861 ccctgaccgc cattgagaac tttgccagca gtattttcag tgccggaatg gatttcctgt
     7921 ttacctgggg agaattcatc ggggtgatca gcatgattac cctgtttgcc cgcgcccgtt
     7981 cagccggtcc ggtaaaaatg tctcccggaa aatttattgc ggggatgtta accagctgca
     8041 tgctcgtatc actcccggca atgattaacg ccggaggcgt acaaatggga ttcagggctg
     8101 actcatttgg cccgattgca tatgtacaac ctcagacgtt tggcgctgct gctggcgcag
     8161 caaatgcagt tctgtcgctg gccaagctgg ccggtgttgg atttgtaatg aacgggatat
     8221 ccatctggcg caaggccgga ctcgatggac ataccgcact gagtgccagt gaatccgtca
     8281 gtaagggaaa cgtcaaattt attgccggcg tcctgctggt ctttatcgat cgtgtcctca
     8341 atgccctgct ggcaagcatt ggaatcgtct tctgattttt ttcaccttca tataaaccac
     8401 tgatatatcc atttaaggag aaactatgtc acgtattcgt gtcgtagcgg cccgtctgta
     8461 cgccgcattt atccacgcac aactgtttgt aatgtcatgc cggagcaaac tgactaagtt
     8521 cctgctgctc attcccatga tgttgctgcc caaagccgtt cttgccgatg gagatcttgc
     8581 cgatatggtt cgcaatgtcg aacaaggtgc caaaacagca cagtcatcat cactgactat
     8641 tgcgcagttc atcggcgtaa ttctgtttct cgggggcctg attggcctga aaaaagttgg
     8701 gaaacaaggg gggatgggac tggctagttg cattgtatct atcgtcattg gggcggtact
     8761 tgtcgccgga cctgaaatga tgagtcgcag ccagaaacag ttggggattt cctcaatttc
     8821 cattggctaa tcttgtccag ggcactggca tttatccggt gcccttcctg ttcggcaata
     8881 caaggaggca catatgtctg cctggagccc ggagcacata acgttacgac gaacagaaaa
     8941 cggcaatgtg attgcccacg acagtcggga cgatcaggaa ttcatttttg cagaatgctg
     9001 ttccgatgaa atcgcaaaca tcattctgga agccgtgaaa cgttacacca gtacggagcc
     9061 tgctcatgca acacagcatt aaaaacttgt ggttgtatcc ctttccagaa attgatgtgg
     9121 ttcatacaca ggagccactg ttaccggaac cggaattaac aacgcccggt cgatgtatct
     9181 gctgccggca aaacgtcagg caccgtttca gactggatga ctcatggcca ctcagacagc
     9241 ttaccgacac aatcagtgac acacgagttc gtcttaataa ggccacagaa catctggtta
     9301 agttaatcag gcgtggtgaa ccagtcgcaa ccggcgaaaa ggaaaagtac aacacggcag
     9361 taaaagccgc tgaacgggcg cttgagcagg cccgtctgtc tgccagacgt ctttcactcc
     9421 gccatgtaca gaaagctgaa atcaccagta cagagccgtt gtcagagaaa gagcaggaac
     9481 tgtttcatga agacggcccg ccttacagct tatgtgcttt ctgccacgcc tggcattcac
     9541 tgaatggtta tgccgcagca cagggcgtta tggtgtggct acccgacctt catccatcca
     9601 cagtcgtggc ccttaaccgc cgttcattac aggaagtttt cagtaatgat aagttccggg
     9661 tccggcgggg acgggaagcg ctctctgcac tgatgcaaaa cagactggcg gttgaggata
     9721 aattccgcag tttccgcccg gccgatttcg ctgacgtttt tcgtcgctat ccaccttccg
     9781 gtcgttcgcc cctgcgtgaa aaaatgaacg ggattgccct gattctgaca ccagattcat
     9841 tcatcaaaaa agaatacgtt gattgatttt tcttcatgcc actccagcgc ggggtggcat
     9901 taaaaaaatc aaactgcaca aacctccctc ctgtaagtcc ggagttccct cacctatgaa
     9961 cataaaccgt ttaatcttca ctatcgaaga ctgtctgaac acgctatccc gtttctctgt
    10021 cgcaagcagt tttgttgaat attgcgattt acgtactgta attggtctgg acagacagga
    10081 tcgtgaacgc cgcccatggc tgaattcgcc ctatattgca gccacaaagc gtggagaata
    10141 tctctctgtc tttgaagtca gcggtgcttt cagggaaatg gatgaagcat ccgaccagac
    10201 tggcccaggt tcactggaat cgctgatcac cagcatgagt gacagcctga atacggcata
    10261 taaaaacagc ggtcataaaa tcagcttcgt ttttgagcgt gatcctgaca tggggaaaga
    10321 agaaatcgaa gacatggtcg ccccacaaaa gcgctcactg gctaatacag gtatccagtt
    10381 acaggatgtg gtcgatgaga aagtcacaac actgtcccca tggctggtcc gggagcgttg
    10441 ctggctggcg atctggagtg gacccgacct tatcagcaac agcgatcgca ccgcccatga
    10501 tgagcttgtc agaagactgg ctgaacgggt accgaaagca cgctttgccc agtccccctg
    10561 gcagtgggcc ttatctgcac tgaaaatccg ccacgaagct tttcttgata atgtggaaca
    10621 ggcactgaga cattcatctg atggcctgat tctccggcta ctggatatac acgaggtcgg
    10681 gcgtgaaata cgtcgtcaga cagaacggca cagcacaccg cgtaactggc aaccccacct
    10741 gcctgaagat gcccagccag cgggatatcg ctggactgac gatgaatccg tactacatgc
    10801 tccttcattg catttgcagt tattcaacac tcaggtgacc acacagggca acctggttca
    10861 ggcgggcgga ctctggcacg gaatggtatc gattacttta cctccgcaaa atctgcagac
    10921 gtttaacgaa ctggtccgtg ccgtcccccg cgcagtcccc tggcgcatca gaatggatct
    10981 gatgcccggc gggatgaagg ctcttaatct gaagaaaacg cttctgactt acagcagctt
    11041 catttctgcc gttcgtccaa tgtatgaatc cgttatgacc ctggctgcaa cggatgaaaa
    11101 agaacctgtc tgcataatga ccatcatggc ctccacgtgg ggaaaaacac gtgaaatatg
    11161 cacacgaaac caggccattc tgaaatcggc cattgaaggc tggggcgtat gtggcaccac
    11221 gacaacattt ggcgatcccc gacgcgcctg ggtgaatacc attctcgcgg catccggagg
    11281 ctccggcccg gttcccctgt atcccccgtt gtcacatgcc atctcgcttt tcccgcttaa
    11341 cagggcaggc tctgtctggc gaggcaaagg gaatctgatg ttacatacag aagacggttc
    11401 cgcatttgag gttggtctgg cgtcatccca acaaaataaa catacagaac tggcaccagg
    11461 cgatccggga ctgggtaaat cagttctgat taatacgttg tcggaaatac agatatcatc
    11521 tgctcagaaa aacctgccct tcattgccta tatcgataag ggatacagtg ctcagggact
    11581 ggttcagctt attcgcgaca gtcttcctcc ggaaagaaaa gacgaagctg tcggtattat
    11641 tctgagcaat gatccggaat acacccgtaa cctttttgac gtgatgtacg gcgctaaaaa
    11701 gcctattacg ccggaaaaga atttcatgtc atctgtgctg tgtgctctct gtgtcgatac
    11761 cggtaccggt caaccctgta accccggaga cacccgacaa attatcaacc agcttatcga
    11821 gctggctttt aaagagtacg gtgaaaataa tccgcgactt tatcgcgcct caacggaaga
    11881 actggtggat tcagcactgc aggactcggg actgtatgaa aaacatgatg cagcctggtg
    11941 ggccagatca acctggttcg aagtccggga tatgctccac aatgccgggt atatcatggc
    12001 ggctcagcgg gcacattatc aggcaatgcc ccagcttccc gaagtgtcct cgatgctggg
    12061 ccataccagt ctcagggatg ttttcggtac tgttcagcgt gatggttcaa acgaacttct
    12121 gctggactac atccgccgcg ctctcgagca gggacataac gactacccca tgatatccgg
    12181 gtatacccgg tttatgatca acccggaaac ccgggtcatc gctgtcgatc tgaataatgt
    12241 tgccggtgac aaaacgcctg caggcaggct taaaaccggc atcatgtacc tgcttgcagg
    12301 tcagatcgcc ggtggtgact tcaccctgcc acaatacagg gatgaagtgt taaagcagct
    12361 tccacgggaa taccatgaaa tcgctcttaa acgtattaac caacttgatc aggaggttaa
    12421 gacaaaagtt tatgatgaac tgcataacgc ccggggcatt gacttcatct gggaaaatct
    12481 ggatacccag gagcgtgagc aacgcaagtt tgctatccgc acggttctga gtacgcagta
    12541 tctgagggac tacccggaaa gcgttcttaa gtcagcaaat actctctggt tactgcgcta
    12601 caaacccgaa gatatccccg tactgaggga taacttcaac gtgcctgaat ttatgctgaa
    12661 acgcttcctg aaaatgccgg aaggcccggc accagatggt agtggtgttc ctgttctggg
    12721 agtattcagg gtaaaaagtg gcacgcttgc gagaatactc aaatttaccg tcggtccact
    12781 ggagctatgg gcgctgaact catcgccaaa agacagtgcc ctgcgtaaaa cactgacaaa
    12841 taagttaggt tcagttcggg cacgtaagat tctggccgaa aacttcccca gaggttctgc
    12901 aacgtcatta attgagcacc gggcgggcca acataacagt gacaatgtta ttgaagaact
    12961 ggcctcggag ctcatccgaa aacagggata caatctctga tgtgtacaat tcatatcacc
    13021 ccacgctgcc ggagacctgc cagagggcag gtatttctta aaaaacttga ggtaaaaggt
    13081 gacagtttct cattcactct gccgtcacgt gtcttcaggt tacatccggc ccccaatccc
    13141 agagtgatat acgcctcgga tgccagaaca cagggaaagg ttacaggcat gcggctgttg
    13201 ttgccgtcca gcacagatga gctgataaca acggcaatat ggcaggtttc tgtcccggat
    13261 gaagcaccat tcgacgttca gcatgtcatc cagtggatac tcccttcacc gctggcttcg
    13321 ctgatgtccg atgatacctg gttatggcca tccgttcccg gaacgccggc actgagtgcc
    13381 ggtgactggc ctgtaatgga tactgaccct tccaccttac ggcgcgtcag acgtcgttct
    13441 ttcagcatca tcgataaccg gaacaggaaa ggttatatca ccaggcgata tcagtgtttc
    13501 ccactggccc cactacctga agacagaaaa tacgacagcc ttcctgacct tgtgctccac
    13561 cccgagcagg agacaataaa atgcaaagaa aaacattact cgcagccctt atagctatcc
    13621 tgaatggaac ggcataccag gctcatgctt attcagtcac cgtcgtcgcc agccgtcccg
    13681 ttgaacagca ggtcgtcccc agaatggagg ccatcaaagg tgtgctgagt gacatactga
    13741 gtacccagac agccacgggt accgctatca atcagaactc tgaaaagctg gcaagtgtca
    13801 ttgcccagaa cggacaggcg acacgtcagc agatgatttt cagtaatgaa actcagcgcc
    13861 tcgaggaggc acggaaaagc ttcaccgtgc cagactccat ctgcagtgaa tccgcatcag
    13921 ggattgcaac agaaagcaaa agtgcctcag catccgcagc ctcaaaactc agtaaaggtg
    13981 gcggtgtgag caaccgtagc atccgcgacc gtctggccag cgcagcaaac tctcccgtgc
    14041 gggaagctta tgatggcgcg gccatacacg ccagttactg tacagaagca gaatatgcac
    14101 gttttggagg aaccgccgtt tgtccttctg tgggcgaaat accaggtgga gacagtcagg
    14161 tacgctcgat ttaccacggc gcaggtacag cggacacacc tgcggcactg acatgggatc
    14221 agaagcaaat agatgcagcg acggcttaca tgaaaaacac ttcccgcccc tctgccggtc
    14281 gtgctctggg taaaggagaa gtgaataccc agagtgggcg aacctacgtc ggactccaga
    14341 acgaatacaa cggcatcatt gattctgcgt cgaatcccca actgacgttg attgctgaca
    14401 gcacgcctaa tgaaagcaca cgtaaagcac tggctgaaac actgcagtca gactcagcgg
    14461 ccgcttactt cgaccaggtg gcttcaccag aagcaaaagc acggggatat atgagtaccc
    14521 gtgagtttga agcttttgag gctggccggc gctacgccaa cacagcatac ctggtggatt
    14581 tacaggaaat gcagggggat aacctgctcc gtgaactggt aagaatcact gcgcaaatga
    14641 actggcagct gaacgatctg aaagaacaga tccgccaggg taatgttatc tctggccagc
    14701 aactggctct gactgcccgc cagtattatg aaaaacaact cggtagcctt gagaaaacaa
    14761 tcaatcaggc aaacgcacgt tgaaagctga cgtcagaaca ggactgaaaa aatgaataag
    14821 ttaccggaaa attcagacca gacgaaaaac gtcaaaaaat caccatccat aacccgcata
    14881 gccacccgga ggattattaa catttttgtt ccgctgatgg agactcgcct gataacgaat
    14941 tctgtacagc atatgctgga acaacaaaaa cgcaggctgg acagatttcg tcaggtaaat
    15001 aataaaaagg cgcaactcca cctcacgtgg gaagaagctc tgcaggccag ccatatgtca
    15061 gtagacgatc tggatcgtcg tttccgccgg cgcagaacag tatggcgatt ctgttgctgg
    15121 agtctgctgg ccatcgcttt gtttctcagt ggtatgctct ttgccgcctc ttcattacct
    15181 cttacgactc tggtacgcgc catcagcact cttgtgttga tactgtcagg cgtcgctctt
    15241 tgtgcttcca gagcactgat tgttacatac cgcctctggc agttgcatga acgaaaagtc
    15301 agcgaacccg agcaggggac tttccgtgat tttctgaacg accgcaatgg ctggcgcaat
    15361 gccacattaa ttgccgtaac cagtaaacaa tactgatcac gtccagatgc cagtaccagt
    15421 ggcatgtcgc catccccctt aattaaatcc tgtgtggcct ttgcccatac gcaattctat
    15481 tccggagtta ctgtgaaaat tttacttcgc gcactctgtg caggccttgc tatatcctca
    15541 ctgccggcaa tggcttcagt gacctatcag gatattgtgt ctgccgccac caatcctgac
    15601 gatctgtccc gccaggcgct ggtcacgata ttcggtgatg tggtcaccaa tccactgtca
    15661 accagcgacc ccaccctgat cggaagcatg ttctgggtgt tcaacaatat cattgccgta
    15721 cttgctatcg tctggtgtat cttcattagt atccgccatg ttgtgcgttc ggggcatcag
    15781 ggacaggtat tcagcgtcgg gcgcgatatt gtcggcatac tgagtttcgt cgcaggtttt
    15841 ctgatgatcg ttcctaccgg aaatggctgg agtctcgctc agctcattat gttatggggc
    15901 gcatcgataa tgggggttgg gtccgccaat gtgatggtgc aagttgccgc tgataatatc
    15961 gcaaatggtt attcaatgac tgtacagcca gttcaggcat ccacacgaac agcagcgcgt
    16021 ggcatttttg aaatggaact gtgtaagtat gcggttaacg cagggctaaa tgattttaat
    16081 cagacggcca aatcctcgac ttcactgatg acagaatcag ctaaaaccgc cagtggtaat
    16141 tacactgtca caatatccaa tggcagcggt atctgtggta ctgccagcct gtcagtagaa
    16201 gggaacggaa caacagatca gtcgacgatt ggaaaattct ttaatccctt cagtaaaaac
    16261 gaatacagcg gtgttatctc agcacaacgt gcagccatgg ataatatgat tagcgacatg
    16321 gacaacgctg ccagtgagtt tgtgacaaca tttctggaaa aaagaaattc tggcaacgga
    16381 acactgccgg atatcgagac acgaattcag aaagcagccg acgaatacga acgtgctgtg
    16441 caaaaatcgc tacccactga taacggagag caatccagaa aagaggctct taaatcttat
    16501 ctcacaacct acgggtgggt aacgcttggc gcgtggtatc agacctttgc gacagctaac
    16561 caacgtctgg cagagctggc tgacagagct ccagctgtaa cctcaatgtc atcactggga
    16621 gaagttggtg atactgactt attcagtgca gtcatgagtg cctacaagac tcaattacag
    16681 aacaccacct atacaccacc tctgggcact gttacatcag ccaatgagca acgaatggcc
    16741 aatgcgcaag atcctaagtc tgccttatct gaaatgatga gcttctttgt ctatcgaacc
    16801 aactggatag caacagagat aagtggcaca ggaacaacaa gcgatcaggt caatccattg
    16861 ataaagatga aaaatattgg tgattataca ttaggaacaa cagagattct ctggagtgga
    16921 tatacaacag ttagagttct cgcgacagta ggtgataact ctattttcgg aaaggttgcc
    16981 aatttattct ctggattacc taaaggatta gttgctctgc tggatgctgc agctccccca
    17041 atatatttct tgttgttcct gttgttttgt gcgggttttt ccttatcaat ttacctacca
    17101 tttataccat tcattttctg gatgacaggc attgggaact ggatagtcag tgtattaata
    17161 ggatgtaccg ccggacctct gtgggctgca acacatctcg gaacatcaga agacagagga
    17221 agtcgggcag cctacggtta tatatactta attgatagta tgataaggcc gcccatcatg
    17281 gtctttggtt ttttctttgc ttctgttgcc atcatcgccg taggcacaat actgaatgca
    17341 ttatttggcg ctgccctggt taacgtacag ttcaattcgc tgaccggtat tttcagtctt
    17401 gctggtttct tgctgatata cgccagaatt tgtacaacca ctgtggctgc catatttgcc
    17461 cttcaggcct acctacctga tcatgttatt aatttccttg gtgggcgtga gggagccaac
    17521 actcttggca gcatggcaaa ttctgttaaa gaaatattta ttggaggaag tcgcaatatc
    17581 cgacatactc ctggaatgaa aacagatcgt cttaaaaata atacaaatgg aagtgacgac
    17641 aaggatggaa taaaaggata atataaatat tgcacaacct ccccatcggc attaaaataa
    17701 tagatagtac gaggggaggc attgtatgaa agctaaaaaa gagacacctg acagatttcc
    17761 aacttggtgg ttgttatatt atgtattaag gaaggcttac ttctttttag gaataccttt
    17821 ctttttattt tgcgcactga catctacact gatgttattt agttctcgtt attatggtga
    17881 caacattgaa gattatgttg tcacttttgg tagttggttc ttattacttg cgccaggcat
    17941 atggatgtac tcacgtgcaa aaacaaggag agaaaaaata agaaaagttg tacaaacaat
    18001 aaaagagtca ggcttctact ctccagagaa ggggtatgaa ggattatctt taactcaggg
    18061 agcttatttc ggtattgacc ttaaaaatgg cacaatgttg tatgtccgta tttatccagg
    18121 caacattatg gatgttatcg gttttgacat tcataatttc actcgtactg taacagacga
    18181 caaaacactg gaaattcata cgaaatacat caatctgcca atggttccta ttccatcatg
    18241 gtgtactcat ccagaaaccg catcaaacac catgcacgct atggcaagtc gaggatatga
    18301 ttaccctgtc gactttcctc gtctgattca ggaaaagcgt aaggagtggg agcagattgc
    18361 aggcgtgcct gtagccgaag ttttttaaga taagctcccc ccataacagc cggttcaaat
    18421 gagccggctt ttgttcatta caacagataa cttatgccgt taccgataaa ttcgaccaag
    18481 tgctttaatc gcagcaattt ttatttccat atcatcactt tgactcaatc tctctaattc
    18541 acgaatgatg ttatccgcct ggcatcgacc ttcacctaat gcatagatcg cagcaagcgt
    18601 aatgtctttt gatttactct tcatcatttc aaacaagaaa gtattagcta tcgcaggagg
    18661 catgctcata atcaatcctc ttcatgaaat aaacaatggc actcacttac aaacaggagt
    18721 ccactatcgc aagcgactca acttagtaat atctctcaat ttgaatgaaa aaaccagatg
    18781 gcatgtgaca gggaaccgtc cgcatgatgt ccgtgaccat ttaaccccat aaactgataa
    18841 aaaatgaata ccggtatctc aatgcgaaga aataatctcc gtagaatccc ccatgagaaa
    18901 aatatcatat gggagaaaat cgcaattatc acagccagcg cattcattat tttcgctgtg
    18961 ttctggcatt aattattttc aacacaaaaa ggaaaaaaca atgaatctgg tagacgcctt
    19021 tgtcaaaaaa gtaatatccg gtccatatga agaatatgga aaatggtgga tagatgttga
    19081 atacatttca tggggtgttt ccgggaaaac tagactgatg tttgactcaa aggagcaggc
    19141 actggaagtt aaggaagggt acaaattttt aacgtgagat ctctttttgt taaagggcag
    19201 gaagcggtaa ctccctgccc ttagttacgt ttccaggctt atcagtcgac aagttcccgg
    19261 atcagttcca gaacagcaat cagcgcgttt gcaacggcaa ctactgccct gagaacgatg
    19321 attaacagat taatcatccg cttctccatc actggaggaa atcaccgttg cttactctta
    19381 cattgcctgt cacaacggca ctttcagtgt tagttgaaaa gagacttcct cctgccagag
    19441 cactgaacac agcaccacct gtttgttcag ccaggactta aaatcttgca tccatagtcc
    19501 cgatggatat cacggcagaa gtgtcgccag acagagctaa cctgcggatt ataaggatgg
    19561 ttgctatcaa atgaacagct cgctattata agaacgttag ttgaaaagag atttcctccg
    19621 gtcagggcca cccactctgt aacggaacag taaaaaaacc gacctcacca gtcggttttt
    19681 ttacatctgt tgtttctcaa aatccgcccg acatgcccgt cacaccgggg aaactcatgc
    19741 tctgaatcag aaccaaccat ctcaactttg ccgtctcctg aaaaaactgg cacattcagt
    19801 tcgataacta cccataaata cagagagaaa atccgatgtc agaaaagtgg agtaaagaat
    19861 tcgactcttg ctttcaggtg gggagcatag aagaaattgc agatgctctc gagaaacgca
    19921 gcggaagcga ggaaaatgcg ttagccatgc tggcaatgac agccttcaca ctcatgaccc
    19981 gacgtggtat ctgcgaaatg cagaacgtgg cccccgatgg caaaggcatt cggctggagc
    20041 taatcggctt cagggaaaat gaaacaatac cggatacgct tcattgaaga agaaagggcg
    20101 caatgaggtc agccttcgca acaaagcggc ggcagaaaac ctgccgccgt gttgcccgtt
    20161 aacgtttaac ttcgtaggct aacgttgcca caagcactgt gtttccctgc tggagccgga
    20221 gtccgcaaag cgaatccctc accatccaga caaaacacag aatcgtcaca cagatgacga
    20281 ttaacatcat taaaaacgtt cgctgtggca tcatagcccc tccattgcct tacggcggtt
    20341 atcgggctac tataatgttg tctaggcatt acagtagagc ccttggttga ttgataaaaa
    20401 aatctccagg ggctttctgc tgtatatggt ttagttaaca aagagagcca gtgttccact
    20461 gagcaaaacc agtagctatt catgcttaca attatcttct ccatatctga aataactcaa
    20521 acagcccaat agaaacatat tgttacatct aagatcttga aaagacctct ttgagaaatc
    20581 aatatgagat cacggtatgt tgataggata atatatatga agaaactatt gattcgatta
    20641 atacctgatg caatatatga ggctctggaa aagacagctc tgcattctga acgctccctg
    20701 gaagcacagg ccagatatat ccttagttgc tccgtagata atgaaaaaca attaactggt
    20761 ggtgaacgct atcaaagaga aataaccgcg cgtcttaatc aagcgttatc agaagctaat
    20821 gaggtcataa cagccattaa tcttgttccc gcaagaattg cagaacaatt aggccatcat
    20881 gacgccatag aatccgaaaa ttggttcacc ggaaatgctg ttcctagctt tacagagctt
    20941 gacgaattaa gcgatatctt tggatgctct ccagattggt taaagtttgg cgaaaatgta
    21001 ccttatccaa agagttctaa aggaaggata aactggaatc gtggcggaga aaaagatatc
    21061 gatgcacttt tagagcctga caacaaaggt cgtaaagtct catctattca tattttcagg
    21121 gtaaatgaat ctggcaatat actaatactg
//
LOCUS       DAFNBJ010000082        20686 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000082 DAFNBJ010000000
VERSION     DAFNBJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.082"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(7..1656)
                     /gene="actP"
                     /locus_tag="L2X49_002863"
     CDS             complement(7..1656)
                     /gene="actP"
                     /locus_tag="L2X49_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBN4797926.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1653..1967)
                     /locus_tag="L2X49_002864"
     CDS             complement(1653..1967)
                     /locus_tag="L2X49_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBN4797927.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2168..4126)
                     /gene="acs"
                     /locus_tag="L2X49_002865"
     CDS             complement(2168..4126)
                     /gene="acs"
                     /locus_tag="L2X49_002865"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBN4797928.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDD
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4518..5954
                     /gene="nrfA"
                     /locus_tag="L2X49_002866"
     CDS             4518..5954
                     /gene="nrfA"
                     /locus_tag="L2X49_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBN4797929.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5999..6565
                     /gene="nrfB"
                     /locus_tag="L2X49_002867"
     CDS             5999..6565
                     /gene="nrfB"
                     /locus_tag="L2X49_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBN4797930.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6562..7233
                     /gene="nrfC"
                     /locus_tag="L2X49_002868"
     CDS             6562..7233
                     /gene="nrfC"
                     /locus_tag="L2X49_002868"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBN4797931.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7230..8186
                     /gene="nrfD"
                     /locus_tag="L2X49_002869"
     CDS             7230..8186
                     /gene="nrfD"
                     /locus_tag="L2X49_002869"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBN4797932.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8206..9924
                     /locus_tag="L2X49_002870"
     CDS             8206..9924
                     /locus_tag="L2X49_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBN4797933.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGTWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLELQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9917..10300
                     /gene="nrfF"
                     /locus_tag="L2X49_002871"
     CDS             9917..10300
                     /gene="nrfF"
                     /locus_tag="L2X49_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBN4797934.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10297..10893
                     /gene="nrfG"
                     /locus_tag="L2X49_002872"
     CDS             10297..10893
                     /gene="nrfG"
                     /locus_tag="L2X49_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBN4797935.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASKQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11235..12548
                     /gene="gltP"
                     /locus_tag="L2X49_002873"
     CDS             11235..12548
                     /gene="gltP"
                     /locus_tag="L2X49_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBN4797936.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12626..13315)
                     /locus_tag="L2X49_002874"
     CDS             complement(12626..13315)
                     /locus_tag="L2X49_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBN4797937.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13409..15556)
                     /gene="fdhF"
                     /locus_tag="L2X49_002875"
     CDS             complement(13409..15556)
                     /gene="fdhF"
                     /locus_tag="L2X49_002875"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15137..15139),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBN4797938.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15754..17220)
                     /gene="mdtP"
                     /locus_tag="L2X49_002876"
     CDS             complement(15754..17220)
                     /gene="mdtP"
                     /locus_tag="L2X49_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBN4797939.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHDLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17217..19268)
                     /gene="mdtO"
                     /locus_tag="L2X49_002877"
     CDS             complement(17217..19268)
                     /gene="mdtO"
                     /locus_tag="L2X49_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBN4797940.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19268..20299)
                     /gene="mdtN"
                     /locus_tag="L2X49_002878"
     CDS             complement(19268..20299)
                     /gene="mdtN"
                     /locus_tag="L2X49_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBN4797941.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20318..20593)
                     /locus_tag="L2X49_002879"
     CDS             complement(20318..20593)
                     /locus_tag="L2X49_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797942.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4664 a         5488 c         5667 g         4867 t
ORIGIN      
        1 gagagattaa tgtgcgcggc cttgctcaac gccaaagccg gtctgggagc ggataaactg
       61 tgcacggaac agttcacgct cgcgcgcacc ttccgctgag ttatcggttg ccgagaagaa
      121 ccagatgccg aggaatgcca cgctgataga gaacagcgcc gggtattcat acgggaagat
      181 ggctttttcg tgaccaagga tctgtaccca aatcgtcggg ccgaggatca tcagtaccac
      241 tgcggtaatc agccccagcc agccacccat catcgcgcca cgagtggtca gtttcgacca
      301 gtacatagaa agcagaatga tcgggaagtt acagctcgct gcgatggcaa acgccaggcc
      361 taccatgaag gcgatgttct gattctcaaa cagcacgccg aggataatgg caatcacacc
      421 taaaatcagc acggtgattt tcgatacccg cagttcttca cgttcggtcg cgcctttttt
      481 gaagacgtta gcgtacaggt catgcgaaac cgctgatgcg cccgccagcg tcagaccagc
      541 aaccaccgca agaatggtgg caaaagcaac cgctgaaata aaaccgagga acaggttgcc
      601 gcccactgca ttcgccaggt gaacggccgc catgttgtta ccaccaatca gatggcccgc
      661 cgcgtcttta tattccggat tcgcaccaac cagcatgatc gcgccgaagc cgataataaa
      721 ggtcagaata tagaagtagc ccataaatcc ggtagcgtag aacacgctct tacgcgcttc
      781 gcgggcatcg ctgaccgtaa agaagcgcat cagaatgtgc ggcaagccag ccgtaccaaa
      841 catcagtccc agacccagag agagcgcgga gatcggatct ttcaccagtc cgcccggttt
      901 catgatgtcg acacctttcg ggtgtaccgc catcgcttcg ctgaacagat tgttgaagct
      961 aaagccgacg tgtttcatca ccataaaggc cataaagctg gcaccgaaca gcaacagcac
     1021 ggctttgata atttgtaccc aggtggtcgc cagcatgccg ccgaacagga cgtacatcat
     1081 catcagcacg ccgaccagca ccaccgcaat gtgatagtta aggccaaaca gcagctcgat
     1141 cagtttacct gcacccacca tctgggcgat aaggtaaagc gccaccacca ccagagaacc
     1201 acaggccgaa agaatacgaa tcggcccttg tttcagacgg taagaggcca catcggcaaa
     1261 ggtgtagcgc cccaggttac gcagacgttc ggcaatcagg aacagaatga tcggccagcc
     1321 caccaggaag cccagcgagt aaattaagcc gtcatagccg gaggtaaaca ccagcgcgga
     1381 gatccccaaa aatgaggcgg cggacatata gtcaccggca atcgccagcc cgttctggaa
     1441 gccagtgata ttgccgcctg cggtgtagta gtcgctacga gaacgtacgc gttttgatgc
     1501 ccagtaggta atgccgagcg taaacacgac gaaaatcagg aacataataa tcgcctgcca
     1561 gttcgttggc tggcgctcta cggccccgct aatagcatcc gcggcgttag ctgcgaaagg
     1621 gagtgtggcg gcaagcgccg tcagaactct tttcatgatg cttgtacctc atgcaggact
     1681 tcgttattaa gacggtcgaa ttcgccgttc gcccgccaga tgtagatacc ggtgagaaca
     1741 aaggagatca caatcactcc aacaccaatc ggaataccgc gtgtgacgct ggtgttcgga
     1801 ttcagcggcg tgcccagcca gccgggcgcg aaggcgatca gtaaaataaa gccgatataa
     1861 actgccagca taataatcga caggatggtg gcaaaccgtt gccgtttttc gactaactcc
     1921 ctgaaatgcg cattgtcttc tatccgctga taaatagtgc cattcatcac agattctcca
     1981 gaggtaatgt agggattgtt ttaattcccg ctcccttatg ggagaaggtt aacgctcggg
     2041 taacccttgc cgaatgtagg ccggataagg cgttatatgc cgcatccggc aatcaatgcc
     2101 tgatgcgacg ctgtcgcgtc ttatcaggcc tacaaaccgt taccgattcg catccggcaa
     2161 ttgtgggtta cgatggcatc gcgatagcct gcttctcttc aagcagcttc tcgacaacgc
     2221 caggatcggc aagcgtcgag gtatcgccca ggttgctggt atcgcccgcc gcaattttgc
     2281 gcagaatacg gcgcataatt ttgccggaac gggttttagg cagggagtcg gtccagtgca
     2341 gcacgtctgg cgtcgccagc gggccaatct ctttacgcac ccagttgcgg acttctgcgt
     2401 acagttctgg tgacggttcc tccccgtgat taagcgtgac gtaggcgtag atcgcctgac
     2461 ctttaatatt gtgcggaata cctactacgg cggcttcggc aatcttcgga tgcgccacca
     2521 gcgccgactc aatctctgcc gtccccagac ggtgaccgga gacgttcagc acgtcgtcca
     2581 cacgcccggt gatccagtaa tagccatctt catcgcgacg cgcgccgtcg ccgctgaaat
     2641 acatattttt gaaggtggag aagtaggtct gttcaaaacg ttcgtgatcg ccaaacagcg
     2701 tacgcgcctg acccggccag gagtcggtga tcaccaggct gccttcggta gcgccttcca
     2761 gcgggttacc ttcgttatcg accagcgccg gttgcacgcc gaagaacgga cgtgttgccg
     2821 aaccggcttt cagctcggta gcgccaggca gcggcgtgat catgaaaccg ccagtttcgg
     2881 tctgccacca ggtatcgacc accggacatt tctcgttgcc gatttttttc cagtaccact
     2941 cccacgcttc tgggttaatt ggctcgccca cggaaccgag aatgcgcagc gacgaacggt
     3001 cggtaccctc gatcgcttta tcgccttccg ccatcagcgc gcggatcgcc gtgggtgcgg
     3061 tatagagaat attgacctga tgcttgtcca ccacctgcgc catacgggca ggcgtcggcc
     3121 agttgggtac gccttcaaac atcagcgtgg tcgcaccgca ggccagcggg ccgtacagca
     3181 agtaactgtg tccggtcacc cagcccacat cggcggtgca ccagtagata tcacccggat
     3241 gataatcaaa gacatattta aaggtcagcg ccgcgtacac cagataaccg ccggtagtat
     3301 gcagcacacc ttttggctta ccggtagaac cggaggtgta gagaataaac agcggatctt
     3361 cggcgttcat ctcttccgcc tggtgctgat cgctcgcttg ctcaaccagg tcgtgccacc
     3421 acaggtcgcg cccttcctgc cagtcaattt tcccgccagt acgcttcagt accaccacat
     3481 gctctacgct ggtgacgttc gggtttttca gcgcgtcatc aacgtttttc ttcagcggaa
     3541 tactgcgccc ggcacgcaca ccttcgtcgg aagtgatcac cagtcgtgag ttggaatcaa
     3601 taatgcgccc ggcaacggct tccggcgaga agccgccgaa aatcaccgaa tgcaccgcgc
     3661 caatgcgggc gcaggccagc atcgcaaccg cggcttccgg caccatcggc atataaatcg
     3721 ccaccacatc acctttttta atgcccagct cgagcagggt attggcgaag cggcagacgt
     3781 cgcggtgcag ctctttatag ctgatatgtt tgctctggct gtcgtcgtcg ccttcccaga
     3841 tgatggcggt gcgatcgccg ttttcttgca gatggcggtc aaggcagttt gccgccagat
     3901 tcagcgtgcc gtcctcgtac catttaatgg acacattacc gggggcaaag gaggtatttt
     3961 tcaccttctg gtacggtttg atccagtcga gaatttttcc ctgttcgccc cagaaggtat
     4021 caggtacgtt aatagattgt tgatacatcg cctcgtactg ctgagggttt atcaggcaac
     4081 ggtctgcgat gttggcagga atggtgtgtt tgtgaatttg gctcatgctt ttgttctcct
     4141 tgtaggatgt taataatatg tggcataagc gttaaatgta ggggtattgg cagttttgtt
     4201 tagtatttgg gcgacagatc acgcaaaagt agaattgtgc aaataaacgg cagggtaatt
     4261 tttgaaggtc accaacaaaa gttgattaat tctttgagga acatgcagtt atgcatgctg
     4321 ttgaaaaaga ggaagatact gactaactct aaagtggtat tttacatgca cttacaattg
     4381 attaaagaca acattcacag tgtggttatt tgttacacat aggggcgagc aatgtcatga
     4441 cagtgtaggt gcggttactg tcgtgaaaac aataaaaacc gccattgcaa caatggcgca
     4501 attcggatga agcccctatg acaaggataa aaataaacgc acgccgtatc ttcagcttat
     4561 tgattccttt tttctttttc acttctgttc acgctgaaca aacggcagct cccgcaaaac
     4621 ctgtaactgt ggaagcgaag aatgaaacct ttgccccgca gcatcccgat caatatctct
     4681 cctggaaagc cacctcggaa cagtcagagc gtgttgacgc cctggcggaa gatccacggc
     4741 tggtgatcct gtgggcgggg tatcccttct cgcgcgatta caacaagccg cgtggacatg
     4801 cctttgctgt gaccgatgtg cgtgaaaccc tgcgtaccgg tgcgccgaaa aacgctgaag
     4861 atggtccgct accgatggcg tgctggagtt gtaaaagccc ggatgtggcg cgtctgatcc
     4921 agaaagacgg cgaagatgga tacttccacg gtaagtgggc gcgcggcggc ccggaaatcg
     4981 tcaacaactt aggttgtgcc gactgccata acaccgcctc accagagttc gccaaaggca
     5041 aaccggagtt aaccctttcc cgtccgtatg cggctcgcgc gatggaagcc attggtaaac
     5101 cttttgagaa agccggacgt ttcgaccagc aatcgatggt ttgcggtcag tgccatgtgg
     5161 agtattactt cgacggcaaa aacaaagcgg ttaaattccc gtgggatgac ggcatgaaag
     5221 tcgaaaatat ggagcagtat tacgacaaaa ttgccttctc tgactggact aactccctgt
     5281 cgaaaacgcc aatgctgaaa gcgcagcacc cggaatatga aacctggaca gcgggcattc
     5341 acggtaaaaa caacgtgacc tgtatcgact gccatatgcc aaaagtgcag aacgccgaag
     5401 gcaaactcta caccgaccat aaaattggta atccgtttga taacttcgcc cagacttgtg
     5461 cgaactgcca tacccaggac aaagctgcct tgcaaaaagt ggtcgcggaa cgtaagcagt
     5521 cgattaacga cctgaaaatc aaggttgaag atcaactggt tcacgctcac ttcgaagcga
     5581 aagcagcgct ggatgcaggc gcgacggaag ccgaaatgaa gccaattcag gacgatatcc
     5641 gtcatgccca gtggcgctgg gatctggcga tcgcttccca cggcattcat atgcacgcac
     5701 cggaagaagg tttacggatg ctcggtacgg cgatggataa agcggcggat gcacgcacca
     5761 aactggcgcg cctgctggcg accaaaggca tcacccatga aatccagatc ccggatatct
     5821 caaccaaaga gaaagcccag caggccattg gcctgaacat ggaacaaatc aaggccgaga
     5881 agcaggactt catcaaaacg gtgatcccgc agtgggaaga acaggcacgt aaaaacggtc
     5941 tgttaagcca ataaccccgt tccgcctcgc aaggggcgga aaacacaatg gagtgaatat
     6001 gagcgtatta cgttcgttat taactgccgg ggtgctggcg tcgggcctgt tgtggagcct
     6061 gaacggaatt accgctaccc ctgcggcgca ggcatctgat gatcgctacg aagttaccca
     6121 gcagcgtaac ccggatgccg cctgtctgga ctgtcataag ccagataccg aaggtatgca
     6181 tggaaaacat gcttccgtta tcaacccgaa taacaaactg ccggtcacct gcaccaactg
     6241 tcacggccag ccatcaccgc aacaccgcga aggggtgaaa gatgtgatgc gctttaacga
     6301 gccgatgtac aaggttggag agcagaacag cgtctgtatg tcctgtcatc tgccggaaca
     6361 gttgcaaaaa gcgttctggc cgcacgatgt ccacgtaacc aaagtggcgt gcgccagctg
     6421 ccattccctg catccacagc aagatacgat gcagacgtta agtgacaaag gacggattaa
     6481 gatttgcgtc gattgccaca gcgatcagcg caccaatccg aactttaacc cggcgtctgt
     6541 tccgttgctt aaggagcagc catgacctgg tctcgtcgcc agtttctcac cggcgtcggc
     6601 gtgctggcag ccgtcagcgg caccgcaggg cgcgtggtgg cgaaaacgtt gaatatcaat
     6661 ggggtgcgtt acggcatggt gcatgacgag tcgttatgca tcggctgtac cgcctgtatg
     6721 gatgcttgtc gggaagtgaa caaggtgccg gaaggcgtct cgcgcctgac gattattcgt
     6781 agcgagccgc agggcgaatt tcctgatgtg aaatatcgct tcttccgtaa gtcttgccag
     6841 cactgcgatc atgcgccgtg cgttgacgtc tgcccgaccg gtgcgtcttt tcgtgatgcc
     6901 gctagcggga ttgtcgatgt taatcctgat ctctgcgtcg gttgtcagta ctgcatcgcc
     6961 gcctgtccgt accgcgtgcg ctttatccat ccggtcacga agacggcgga caaatgcgat
     7021 ttctgccgta aaaccaattt gcaggccggt aagctgcccg cgtgcgttga ggcttgcccg
     7081 acgaaggcgc tgacgtttgg caatctggac gatcctaaca gtgagatttc gcaactgctg
     7141 cgccagaagc ctacttaccg ctacaagctg gcgctgggaa ccaaaccgaa gctgtaccgc
     7201 gtaccgttta aatacgggga ggtgagccaa tgacgcagac ttccgcattt cattttgaat
     7261 cgctggtgtg ggactggccg attgccatct acctgttttt gattggtatt tctgccggtc
     7321 tggtgacgct ggccgtgctg ttacgtcgct tctacccgca ggcgggcggt gcagacagta
     7381 cgttgctgcg caccacgctg attgtcgggc cgggcgcggt gatcctcggt ctgttgatcc
     7441 tcgtcttcca cctgacaaga ccgtggacct tctggaagct gatgttccac tacagtttta
     7501 cctcggtgat gtcgatgggg gtgatgctgt ttcagctcta catggtggtg ctggtgctgt
     7561 ggctggcgaa aatctttgaa catgatttgc ttgccctgca acaacgctgg ttgccgaagc
     7621 tggggatcgt gcaaaaggtt ctgagcctgc tgacgcccgt tcatcgcgga ctggaaacat
     7681 tgatgctggt gttggcggtg ttgttggggg cttataccgg ctttctgctg tcggcgctga
     7741 aatcgtatcc gttcctcaat aacccgatcc tgccggtgct gttcctcttc tccggcatct
     7801 cgtccggtgc ggcggtggcg ctgatcgcca tggcgatacg ccaacgcagt aacccgcatt
     7861 ccacggaagc gcagtttgta caccgtatgg aaatccccgt ggtatggggt gaaatcttcc
     7921 tgctggtggc gttttttgtc ggtctggcgc tgggcgatga cggtaaagtg cgtgcgctgg
     7981 tggcggcatt aggtggcggt ttctggacgt ggtggttctg gcttggtgtc gccgggctgg
     8041 ggctgattgt gccaatgttg ctcaaaccgt gggtcaatcg cagttccggc attcctaccg
     8101 tgctggcggc gtgtggggcc agtctggtcg gcgtgttgat gctgcgcttt ttcattctct
     8161 acgccgggca gttaacggtg gcgtaagcca gaaaagaggt ggtttctgga cgtattcctt
     8221 cctgaagtcg gttttctggc gttgttgtta agtctcgggg tcaacgtgtt gaccccgttg
     8281 acggcctttg cgggagtgcg gttgcgctgg cctgccatga tgcgactcac ttgcatcggc
     8341 attctggcgc agttcgcgct cctgctgctc gcctttggcg tactgacgta ttgttttctc
     8401 atcagcgatt tctcggtcat ttatgtcgcc caacatagct acagcctgct gtcgtgggaa
     8461 ctcaaactgg cggcggtgtg gggcggccat gaaggttcgc tgctgctttg ggtgctgctg
     8521 ctttccgcct ggagcgcgct gtttgcctgg cattatcggc agcaaaccga tccgctattt
     8581 ccgctgacgc tagccgtttt atctctcatg ctcgccgcac tgctactgtt tgtagtgctg
     8641 tggtccgatc ccttcgtgcg gatatttcca ccagcaatcg aaggccgcga tctcaatccg
     8701 atgctgcaac atcccggtct tatctttcat ccaccgctgc tttaccttgg ctatggcggt
     8761 ttgatggtgg cggcgagcgt ggcgctggcg agtttactgc gcggcgagtt tgatggtgcc
     8821 tgcgccagaa tttgttggcg ctgggcacta cctggctgga gcgcattaac ggcggggatc
     8881 atcctcggta cctggtgggc ctattgcgaa ctgggctggg gcggctggtg gttctgggat
     8941 ccggtggaaa acgcctcttt attaccctgg ctttctgcca ctgcgctgct gcacagtttg
     9001 tccctgacac gccagcggga gattttccgc cactggtcgc tgttgctggc gatagtaact
     9061 ctgatgctgt cgctactggg taccttaatt gtccgttctg gcattctggt ttcggttcat
     9121 gcgttcgcac tggataacgt ccgcgccgtg ccgttgttca gcctgtttgc actgattagc
     9181 cttgcgtctc tggctctgta tggctggcga gcgcgggacg gtggtgcggt ggtgcgtttt
     9241 tcggggttat cgcgggaaat gttaatcctc gctacgctgt tgctgttttg cgcagtgcta
     9301 ctgatcgtgc tggtgggaac gctttatccg atgatttacg gtctgctggg ctggggacgc
     9361 ctctccgttg gcgcgccgta ttttaaccgc gcgacgttac cgtttggcct gttgatgctg
     9421 gtggtgattg tgctggcgac gtttgtctct ggcaaacgcg tgcagcttcc ggcgctggta
     9481 gctcatgcgg gcgtgctgtt atttgccgcg gggatcgtgg tttccagcgt cagccgtcag
     9541 gagatcagcc tgaatttaca gccgggtcag caggtgacgc tggcaggata caccttccgt
     9601 tttgagcgcc tcgagctgca agccaaaggc aattacacca gcgaaaaagc gatagtggca
     9661 ctgtttgacc atcagcaacg cattggtgaa ctgatgccgg agcggcgttt ttacgaagca
     9721 cgtcgtcagc aaatgatgga accgtcaatt cgctggaacg gcatccatga ctggtatgcg
     9781 gtcatgggtg aaaaaaccgg agcggatcgt tacgcttttc gtttgtatgt acaaagcggt
     9841 gtgcgctgga tctggggggg aggattgttg atgattgcgg gcgcattgtt aagcggatgg
     9901 cgggggaaga agcgcgatga ataaagggct tctcacgcta ctgctgttat ttacctgttt
     9961 tgcgcgcgct caggtcgtag acacctggca attcgccaat ccacaacaac agcaacaggc
    10021 gttaaatatt gccagccagt tacgttgtcc gcagtgccag aatcaaaact tactggaatc
    10081 caacgcgccg gtggcagtca gtatgcgcca tcaggtttac agcatggtgg cggaggggaa
    10141 gaacgaagtc gaaatcattg gctggatgac cgaacgctac ggagattttg ttcgctataa
    10201 cccaccgtta acgggtcaga cgctggtgtt gtgggcgctg ccagtggtgt tgttactgct
    10261 gatggcactg atcctctggc gagtgagggc gaagcgatga aacagcccaa aataccggtg
    10321 aaaatgctga caacactcac gatattgatg gtatttctct gtgtcggcag ttatctgtta
    10381 agcccaaaat ggcaggcggt acgtgcggag tatcagcgtc agcgcgatcc gctacatcag
    10441 tttgccagca agcaaacccc ggaagcgcag cttcaggcat tacaggataa aatccgcgct
    10501 aatccacaaa acagcgaaca gtgggcgtta ctgggcgagt attatctgtg gcaaaacgat
    10561 tacagcaatt cgctgctggc gtatcgtcag gcgttgcaac tacgtggtga gaacgctgaa
    10621 ctgtatgcgg cgctggcgac ggtgctttat taccaggcca gccagcatat gaccgcccag
    10681 actcgcgcaa tgatcgataa agccctcgcg ctggactcta acgaaatcac cgctctgatg
    10741 ctgctggctt ccgatgcgtt tatgcaggcg aattacgcgc aagccatcga actatggcaa
    10801 aaagtgatgg atctcaactc accccggatc aaccggacac agctggtcga gtcgattaat
    10861 atggcgaaat tgttgcagcg gagatcagat taatcatctg gtgatgaaga ttatgttatt
    10921 gagttgtata aaaaataatc gaatgaacat atgccaaaaa taatcaccaa tcagtattat
    10981 tgcagattaa acaaataaaa atctttccat aacaaatggt tattcattaa tcctgccata
    11041 aactcgcgtt attatgcatt aatgcagcga aaagctctgt tgttaaaggg ttgcgcaaca
    11101 taccgcgcaa atgatactga tcataagcgt taaaaaaatc tacaaaccaa cgcaacacaa
    11161 ttcatgccct ggcagtatgt cacgttctcg cgtttctgaa cggggaacgg cgctccattg
    11221 aggaagttat tctgatgaaa agtataaaat tcagcctggc ctggcagatt ctgtttgcta
    11281 tggtgctggg cattctcctg ggaagctacc tgcactacca tagcgacagc cgcgactggc
    11341 tggtcgtcaa tttgctctct ccggcgggtg atatcttcat ccatctgatc aaaatgattg
    11401 ttgtgccgat tgtgatctcc acgctggtgg tgggtatcgc gggtgttggt gatgccaaac
    11461 agctcgggcg tattggcgcg aaaaccatta tttacttcga ggtgatcacc accgtcgcca
    11521 tcattttggg gatcactctg gcgaacgtct tccagcccgg tgccggggtg gatatgtcgc
    11581 agctggcgac cgtcgatatc tcgaaatatc agagcactac ggaagcggta caaagcagtt
    11641 cccacggcat tatgggcacg attttgtcgc tggtgccgac gaacattgtg gcgtcgatgg
    11701 cgaaaggcga aatgctgccg atcatcttct tctcggtgct gtttggtctg gggctttcct
    11761 ccctgcccgc gacgcatcgt gaaccgctgg tgaccgtgtt ccgctccatc tctgaaacca
    11821 tgtttaaagt gactcacatg gtgatgcgtt atgcaccggt gggtgtgttt gcgctgattg
    11881 cggtgacggt ggctaacttt ggtttctctt ctctgtggcc actggcgaaa ctggtgctgc
    11941 tggtgcattt cgccattctg ttcttcgcgc tggtagtgct gggaattgtg gcgcgcctgt
    12001 gcgggttaag cgtctggatc ctgattcgta ttctgaaaga tgagctgatt ctggcgtact
    12061 ccactgccag ctctgaaagc gtactgccgc gaattattga gaagatggaa gcctacggag
    12121 caccggcgtc gatcaccagc ttcgtggtgc cgaccggtta ctcttttaac cttgatggtt
    12181 cgacgctgta tcaaagtatt gccgctattt tcatcgctca gctgtatggc attgacctgt
    12241 ccatctggca ggaaatcatt ctggtgctga cgctgatggt gacctcgaaa gggattgctg
    12301 gcgtgccggg cgtgtcgttt gtggtgctgc tggcaacgct gggtagcgta ggtattccgc
    12361 tggaaggcct ggcgtttatt gctggtgttg accgtatcct cgacatggcg cgtactgcgc
    12421 tgaacgtggt gggtaatgcg ctggcggtgc tggtgattgc caagtgggaa cacaaatttg
    12481 accgcaaaaa ggcgctggct tatgagcgtg aagtgctggg caaatttgat aaaactgcgg
    12541 atcaataatt gaagattgcc ggggttatcc acccggcaat gtgtggatgc ctgatgcgac
    12601 gctggtgcgt cttatcaggc ctaccttaac cgttggttaa tttttcaaac tcttcacacc
    12661 cggtatcaaa cccttccata cagctcaggt tcaaccagtg cagcgccttt tgtttgttct
    12721 tctcgataaa accttcctca ccgtttaaga acatcattcc cgcccagtac tcggaataac
    12781 cggttcggga aattgcagag ctgcgtttga aataccaggt tgccttgtca tcgtcggcct
    12841 taatgcccac gccgttggcg taaatcaaac caagcagcat ttgggcatcg acggcggagt
    12901 cattctccag atcttccgag gcgttttcta acagcgaaat cgcctttgga taatccggtt
    12961 tacccgcttg agtatttacc agaatatgcg ccagcgttac ttcaccttct ttactgcccg
    13021 cttgcgccgc tttctctgca agaacttttg cttgtggata gtccagactg accggattgg
    13081 tgattttgat ctgcgccagc agcgcgcagg catcagcatc accgctgtcg gcggctttct
    13141 gtgcccaata ttcggctttg ctcaaatcgc cggagctaaa ccagctgtcg gcaagaaaat
    13201 attgtgcgcg tcggtccccg gcctctgctg cctttaaata ctggctgcca ggctcggagt
    13261 cgttcgcgtg ggcaaagaat gtcaaaaaca acatcaatgc gataattttt ttcattttga
    13321 taatacgatt gggtagggag taaccagtat aaagagttcg aatggataaa aaaacagcct
    13381 ccgaaaggag gctgtagaaa ggactgtatt acgccagtgc cgcttcgcgc aggcgagttt
    13441 tcaacttgtt gtactcgtca atcacgtact gctcggcggc gcgctgatcg gcgatcggct
    13501 cgacgcgcac ggcgcagtat ttgtattccg gcgttttcgt aatcgggctt aagttttcgg
    13561 taaccagctc gttacaggca ccaatccacc actggtaggt catgtaaatc gcccctttgt
    13621 tcggacgatc gctgacctgc gcacgggtga taattttgcc tttacgcgag tgcacccaaa
    13681 ccaatgcctc atcttcaata cccagacgtt tggcgtcttc ggtattgatt tgtgcgtagc
    13741 caggttcatc agccagcgcc gccagtgccg cacagttacc ggtcatcgaa cggcaagagt
    13801 agtgaccaac ttcacgcacc gttgacagta ccatcgggta ctcgtcggtg agtttgtcga
    13861 ttggcgctac ccagtcgcag gtgaagaact gtgccagacc gttcggggta tcaaacttct
    13921 ctttaaacag ataagaagtc ccctgatcgg catctgaagt atcgcggcaa ggccactgaa
    13981 tgaagcccag ttcgcccatt ttctcgtaag tcgcaccgta gaaatccggg cacagatgac
    14041 gcaactcatc ccagatctcc tgggtgttgt tgtagtgcat cggataaccc atacgggtgg
    14101 cgatttcact gatgatttgc cagtccgttt tcagatccca tttcggttca actgctttga
    14161 agaaacgctg gaagccacgg tcagccgcag taaacacgcc ttcatgctcg ccccacgacg
    14221 ttgacggcaa aatgacatcc gccgccgacg cggttttggt cataaagatg tcctgaacga
    14281 taaccagttc cagatcttca aaggctttac gtactgccga cagctccgcg tcagtttgca
    14341 gcggatcttc gcccataatg tacgcggcac gcacttcgcc atgcgctgcg cggtgcggca
    14401 gctcgctgat gcgataaccg gtatgagcag gcaggctttc cacgccccag gctttggcga
    14461 atttctcgcg gttagccgga tctttcacgt actggtatcc cggatacgta tccggcagcg
    14521 cgcccatatc gcaggcaccc tgcacgttgt tctgaccacg aaccgggttt acacccgcat
    14581 gcggcttacc gaggttaccg gtcagcatcg cgaggctggt cagagaacgc acggtttcca
    14641 cgccctggta gaactgggtt acacccatgc cccacaggat ggcggcgctt ttcgcctggg
    14701 catacatccg tgccgcctga cgaatctcac tggcgctgac gccggtgata tcttcaaccg
    14761 actccggcgt gtagccttca acgattttac gatactcttc aaagccttct gtacgggaag
    14821 cgacgaacgc tttgtcgtac agattttctt caataatgac atggcccatc gcattcaaca
    14881 gcgcgatgtt cgagccgttt ttcagtgcaa tgtgcatgtc agcaatgcgc gcggtttcaa
    14941 ttttgcgcgg atcgcagacg ataattttcg ccccgttacg tttagcgtta attacgtgat
    15001 tcgccacgat tgggtgggaa tccgccgggt tgtacccgaa aacgaacact aaatcggtat
    15061 tatcaatttc gttaatagca ttgctcattg cgccattacc gaccgattgg tgcagacctg
    15121 caaccgatgg gccgtgtcag acacgagcgc agcagtcaac gttattggta ccaataacgg
    15181 cgcgcgcaaa tttttgcatt acatagttgg tttcgttacc cgtaccacgc gaggagccgg
    15241 tcgtctggat ggcatccgga ccgtacttct ctttgatggc gctcaggcgc tcggcaacgt
    15301 aattcagtgc ctcatcccag gaaacaggtt cgagtttgcc gccacgctgg cgacggatca
    15361 tgggggtttt caggcgcggg gtcaggatct gggtatcgtt aatgaaatcc cagccataat
    15421 aacccttcag acacagggta ccctggttgg ttttcccctg cgctgcctcc gcccggacga
    15481 ttttgccgtt atcgacgacc aggttgattt tgcaacctga tgcgcaatag gggcaaaccg
    15541 tgacgacttt tttcatcggt ctcgctccag ttaatcaaat cacgcatacg cgctctcgac
    15601 tacagtatgc atcttttatg ccacatttta tttggggtca ttccctgata ttacgggcac
    15661 tatttattca aaactctgac gaaaaacagg ctgtcgtcag ttttgacgtg acgaaacgaa
    15721 ataccgcgtg acagccatca cgcggcagac attttatttt ttctcgacga cgggagccgc
    15781 ctgatacccg ccgcccagcg atttcatcaa ctgaatgctt tggatcaccc ggcggctgtc
    15841 cagcatcagt aatgacatct cttcggcaag tactggcaac cgggcttcag tggcctgtaa
    15901 gcggctggtt aagccgcgct gataggcggc ctcggcagcg cgctgggtga agcgcgttgc
    15961 ttccacgcgt tcagcctgca tttctcgctc gtcgttgagc gtttgcagac gcgtgccgtt
    16021 gacggcaacg tcacgcaccg cgttcagtac tgactggttg taacgttcaa tcatcatgtt
    16081 gctggcggcg cgcgtgcctt cgagattggc attcaaccgt ccaccgtcaa acagcggcaa
    16141 tttcagaccc gggatgaagt tgaactggcg actggttttt ttgaataagg tatccaggtg
    16201 gatggcatca aggccgaaaa acgctttgat atcaaagctc gggtagaaca gcgcccgcgc
    16261 ggaatccacc tgatctaatg acgcctgaac ataccagcgc atggcttgca gatccgggcg
    16321 tctggcgagc aactcataag agagtgtcgc cggaatgccg gtctggactc gtggtaatgc
    16381 caccggtttg atctccggca tatcgctggc ccccgcgcca atcaatgcac gcagagattc
    16441 tcgcgtttcg gtgatttgcc ctttgacggc agcaatttgt ttatcgaccg ccagaatctg
    16501 tgcccgcgcg ccgtggaaag gcacttgtgc ttccagatcg tgcgccactt tactctggtg
    16561 cgctttcacc gcgtaatcaa tcacatcgcg agtttgttct aacagatcga gcatctgata
    16621 gctggcctgc atactgtaat aaagctgcgc tacgcccgtg gtcagcgata gctctactgc
    16681 tgcggtttct gccagcgcgg cattatgcgc gccaatggcg gcggcaaccg ctgagcgatg
    16741 cacaccccac aaatcaagat ccagtccggc aaacaaacct actgtggctt ccgtatagta
    16801 cggcccgtcc atacccagtg cgggcgcatc catcgcataa gggcttaaaa agccgttcgc
    16861 cgagacgcgt tggcggttga gcatccctaa cgctgcgacc tgtaattgtg aaccggcatc
    16921 taacaaatcg gcctgggact gcgctttttc ttcccgcagt ttcgcttcgg cgagggtgtg
    16981 tgaaccactt agcgtccgtt ggatcagcgc atccagctgc gggtcattga gttgtttcca
    17041 ccactgcgcc tgcggccagc cggagctggc aagatgaata tcgtcggcca gtttgatttg
    17101 ttccggtttg agctgttgat gaggtgcaga atccttacgt accagggcac agccggaaat
    17161 cagcgtcgtg ctgccgagaa tgctgcacaa cagcagacgt gaaagttgac gattgatcat
    17221 tgcgtggctc cttgtgcctg ttccgtggcg ggcgttaatg ccggggctgt ccagtctggc
    17281 aagcgggcga aaagctggca gacgtgttgc tcctgtttta ataaggtggg caggatggcc
    17341 tgcgaggcgg gtgtctcttc aagcgttatt tgaggagaac ggctgagtgc ggttgccaga
    17401 ccggcagcgt atttctccag ggcgtcggca aactgagctg ctctgtccgg ctgtagtgcg
    17461 ttatccagcg cctgcgccga gttccaggtg gcatcccagg cgtgaagaat atcgcggccc
    17521 tgacgaataa ccgtttgcga acgttcaatc agtaaggcgc gttcttcgct gtccagttga
    17581 cgctccagcg ccacgcgttg gcacatctct tcacaggcat taaacgccgc atgcagaccg
    17641 atacgaattt gcagataagt gcgcagagcc gtgacttcct gctggcgtgg gatccgcatt
    17701 actttactta acatacccag cgtgccagcc agtttttgcg gcagtgtgcg cgcttcactt
    17761 tcaggccaga caaaggtgta aatcaccgcg gacaccacgg taccaatgat gatacccatg
    17821 gcgcgatcgc gaatttccac caggtcgtac accgggccaa aaacgttttc gagcgtggcg
    17881 agcgcgaagg tgaccaccat ctgtgtgccg atataagaag agcgttcaga gctggtggca
    17941 atccatgcgc ccaacaggaa aatcggtgcc agcacaaaca gcaattcgac aatattgtcc
    18001 agccagggca tgaccagtag cgtgaataac agcgccagaa tcgcgccgca aaaggccccg
    18061 ccaaaacgca gcaccatctt ctggtacgac gaaccgacat ttgggttagc gacgatcacg
    18121 catgtcagca tacaggtgtg aatgccttcc cagtccacgc cgctgtagaa ggtgtaacag
    18181 atcaaacagg cgagcagtgt ttttaccgcg tagcgcatat agtctggatt ggtaaaagca
    18241 tcggcgacca ttgatggcgg tttggcggcg ggcgttggcg gcgtattcgg gtccatctga
    18301 cccagttgta acaacgtctg gcagatattc tccaggttac attcccgtgc cgccatcgct
    18361 tcactttcag tgatccgcca gtcgctttgc cagcactgac cttcagcaac ggcatgctgc
    18421 agcttattga tttctgaggc taatttttgc cgaaattcaa taattgcctg agaatcagca
    18481 aaagaggtgg gatcgtagcg attcagcgtc gagtaaatgt aggttaccgt tgccacgcag
    18541 ctttgccacc atgcgctttg agttcgccag ttggcatcgt ccgcgagaca aaagacattc
    18601 agtttttgca gcgccagcgc ctctctttca atccgcgttt cgggtagcgg tgcgaggctg
    18661 tccgtcaggt gactaatggc atcatcaagc cgatcattaa gcgcttgatg catttgcgta
    18721 atggcacgac tgggaaacca cagcacgccg attaacgtca tcagcaaggt tggatagagg
    18781 ccaacaacga tacaccacag cgttaagcgc acgaccactt ccggatagtc gagcatggcg
    18841 gggaaggttt gcccgtaaat ggcgacaatg gcgacagcga aaaagaccag tcccagccga
    18901 tgggtgcgca tcaaaaacat acagcccatc aggatcggcc cggcgatgat caatcggatc
    18961 aacggttcgc cgtatgacca tttgtagatc aaaaacaggc tgccgatctc cagcaccgtg
    19021 gcaaccacaa acaagatcgc gacaaatttg gtgtaaaacg cgttcgactg aataccgtaa
    19081 aacagcactg ccagcgataa cgccacaaaa gggatctcaa aagtcatcga gatcagaatc
    19141 accagcaggc agcctaccca gagttgcacg atctgcggca cgcggccggg tcgccgctcg
    19201 cttaactctt catgaaagaa cgccagcagc ctgaccaccg gtaatggcag ggagttgagc
    19261 gcgctcatta ttgcggctca agattagcga ctgccgaagc gccgatgcgg aacatttccg
    19321 ggtcaggttt atcgaccatg attttgaccg gaaaacgctg ggcaacgcgg acccagttaa
    19381 tagagcgaga aactttcggc aggccgccca gcaccaggcc gccgtcatcc ggtagcacgc
    19441 cgtagccaat cgaatccact ttaccctcga aggttttgcc gctgtcactc atcaggcgaa
    19501 tcgttgcggg tgtacctgag cgaatatttt tcagatcggt ttcgcggaag ttggcgatca
    19561 cgtaccagtg acgagtgtcg attagggtaa aaatagggcg catggcagaa gcaaattgcc
    19621 cgacggaggt tttgagggaa atgacccggc catcaaacgg cgcgcgaacg gtcgccattt
    19681 ccagatgaag tttggtcagg gcaatatccg cttcgaccgc cgcacgttgc gccaccagcg
    19741 catcgacgcc gctgacggcg ctggcggctg actgcgcctg taacaatacg gcattaagat
    19801 cggcttctgc ggcgcgctgc gcagttcttg cacggtcaac gtcttccgct gagacaaaac
    19861 cctctttcag taatggctcg gtgcggcgta atgtatctgt ggcctgtttc gcggcggcac
    19921 gggctttttc taccgtggca ttaatcgagt cggcaccaaa ctgttgcgca tcaacgctac
    19981 gctgggtgag cataatttgc ttatccagcg ccgcgaggga ggcttcagct ttcgccagat
    20041 tggcttcgta cgggcgcggg tcaatgcgga acagcaaatc gccctgtttg actgcctggt
    20101 tgtcggtgac cgccagttct acaatgcggc cgctgacttc cggcaccaca tcaatggtat
    20161 ctgctgacgc gtaagcgtca ttagttgatg gcgcactgtc tacgcgccag ataacgaaaa
    20221 caagggcaac cagcgccaat gcaaccacta acagagcagg gaatttactg cgaggagctt
    20281 ttttcggcgt actttccatg agcgcgtctc aaatagatta gaaaaatgcc agccagaaca
    20341 gcatggcgta gagagcaaaa agggcggtat aaataattcc gacaaatgcc agattgatat
    20401 ttgcccgctg aataactcgc ctcgtgatga gcatcaaaat aaggctggca atggcgcaga
    20461 aaaaccagct gggataataa gcaccgatca ccgggattgc cggagataat gagcatcccg
    20521 aaaccatgaa gacgggaatt atccaggctg tttttttgag ttgcatcgcc atgcgagaga
    20581 gaactgttgg catattaaat acatcctgtc tgctgcgaac aatatctgta gtgaatatca
    20641 atatccattg aaggcgtcag tatggctatt ttctcgatga ataaaa
//
LOCUS       DAFNBJ010000083        20583 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000083 DAFNBJ010000000
VERSION     DAFNBJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.083"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            13..486
                     /gene="sbmC"
                     /locus_tag="L2X49_002880"
     CDS             13..486
                     /gene="sbmC"
                     /locus_tag="L2X49_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HBN4797943.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            683..1741
                     /locus_tag="L2X49_002881"
     CDS             683..1741
                     /locus_tag="L2X49_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBN4797944.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWSLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWLIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            1913..2242
                     /locus_tag="L2X49_002882"
     CDS             1913..2242
                     /locus_tag="L2X49_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBN4797945.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(2343..2525)
                     /locus_tag="L2X49_002883"
     CDS             complement(2343..2525)
                     /locus_tag="L2X49_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HBN4797946.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            2597..2725
                     /locus_tag="L2X49_002884"
                     /pseudo
     CDS             2597..2725
                     /locus_tag="L2X49_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(3014..3127)
                     /locus_tag="L2X49_002885"
     CDS             complement(3014..3127)
                     /locus_tag="L2X49_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HBN4797947.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(3140..3319)
                     /locus_tag="L2X49_002886"
     CDS             complement(3140..3319)
                     /locus_tag="L2X49_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797948.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVINKAAENGYSLRVVDDRD
                     STETPATYASPHQLL"
     gene            complement(3316..3690)
                     /locus_tag="L2X49_002887"
     CDS             complement(3316..3690)
                     /locus_tag="L2X49_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBN4797949.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(3780..4106)
                     /locus_tag="L2X49_002888"
     CDS             complement(3780..4106)
                     /locus_tag="L2X49_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBN4797950.1"
                     /translation="MTPPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDTD
                     AYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYP
                     TPETKK"
     gene            complement(4222..4443)
                     /locus_tag="L2X49_002889"
     CDS             complement(4222..4443)
                     /locus_tag="L2X49_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBN4797951.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGGTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4512..4988)
                     /locus_tag="L2X49_002890"
     CDS             complement(4512..4988)
                     /locus_tag="L2X49_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBN4797952.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(5004..5489)
                     /locus_tag="L2X49_002891"
     CDS             complement(5004..5489)
                     /locus_tag="L2X49_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBN4797953.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(5544..6362)
                     /locus_tag="L2X49_002892"
     CDS             complement(5544..6362)
                     /locus_tag="L2X49_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBN4797954.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVEVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(6462..6695)
                     /locus_tag="L2X49_002893"
     CDS             complement(6462..6695)
                     /locus_tag="L2X49_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBN4797955.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            complement(6774..7229)
                     /locus_tag="L2X49_002894"
     CDS             complement(6774..7229)
                     /locus_tag="L2X49_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797956.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            complement(7305..9821)
                     /locus_tag="L2X49_002895"
     CDS             complement(7305..9821)
                     /locus_tag="L2X49_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797957.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            complement(9942..13061)
                     /gene="ag43"
                     /locus_tag="L2X49_002896"
     CDS             complement(9942..13061)
                     /gene="ag43"
                     /locus_tag="L2X49_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBN4797958.1"
                     /translation="MKRHLNTCYRLVWNHMTGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINK
                     NGRQIVRTEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLMRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLTHTSSGLWADIVAQGTRHSMKASSD
                     NNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYN
                     GQATLNVTF"
     gene            complement(13389..14261)
                     /locus_tag="L2X49_002897"
     CDS             complement(13389..14261)
                     /locus_tag="L2X49_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBN4797959.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            15493..16977
                     /locus_tag="L2X49_002898"
     CDS             15493..16977
                     /locus_tag="L2X49_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBN4797960.1"
                     /translation="MSFGQPCDEFPLSALPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISLWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(17299..17901)
                     /locus_tag="L2X49_002899"
     CDS             complement(17299..17901)
                     /locus_tag="L2X49_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797961.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(17988..18194)
                     /locus_tag="L2X49_002900"
     CDS             complement(17988..18194)
                     /locus_tag="L2X49_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBN4797962.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            18953..19102
                     /locus_tag="L2X49_002901"
     CDS             18953..19102
                     /locus_tag="L2X49_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HBN4797963.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            19915..20484
                     /locus_tag="L2X49_002902"
     CDS             19915..20484
                     /locus_tag="L2X49_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBN4797964.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGEFKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERANYLAKEHTKAHCTGERNFGCSRG"
BASE COUNT         4861 a         5154 c         5412 g         5156 t
ORIGIN      
        1 aatggaggcg tcatgaacta cgagattaag caggaagata aacgtaccgt tgcaggtttc
       61 catctcgttg gcccgtggga acagacggta aagaaaggtt ttgagcagtt gatgatgtgg
      121 gtagatagca aaaatattgt gccgaaggaa tgggttgctg tctattacga caatccagat
      181 gaaacacccg ccgaaaaatt acgctgcgac accgtcgtga cggtgccggg ttactttacg
      241 ctccccgaaa acagtgaggg cgtcattctg acagaaattt caggtggtca gtatgctgtg
      301 gcggtagctc gtgtagtcgg tgatgatttt gctaaaccct ggtatcagtt ctttaatagc
      361 ctcttgcagg acagtgctta tgaaatgtta ccaaagccct gcttcgaggt ttatttgaac
      421 aatggcgcgg aagatgggta ctgggatatc gaaatgtatg ttgcggtgca gccaaaacat
      481 cactaattca tctcagggcg gtgtgttaac gcgatgacca ctcttttttt gaaagcgaaa
      541 agagtaagat gcgcctttca attttttcgc tcctgccggg aaattacact gttcccggtt
      601 tgtccgtcgg ataattcaga ggcgcgcctt ctggccgaca gatgagttat gagcgctttt
      661 aatctcatta cggagtttct gcgtgcgtgc cgataagtca ttaagcccgt ttgaaatccg
      721 ggtataccgc cattaccgca ttgtgcatgg tactcgggtc gcgctggcat tcctgctcac
      781 ttttctcatt atccgcctgt ttactatccc ggaaagcacc tggtcgctgg tcaccatggt
      841 ggtgattatg gggccaatct cgttctgggg taacgttgtc cctcgcgcct ttgagcgtat
      901 tggcggtacg gtgttgggtt cgattttagg tcttatcgct ctgcaactgg agttaatctc
      961 gttaccgctg atgttagtct ggtgcgcggc ggccatgttc ctttgcggtt ggctggcgct
     1021 gggcaagaaa ccgtatcaag gtttattgat tggggtgacg ctggcaattg ttgtgggttc
     1081 cccgacaggt gaaattgata cggcgttatg gcgaagcggc gatgtgatcc tcggctcttt
     1141 actggcaatg ttgtttaccg gtatctggcc acaacgggcg ttcatccact ggctcattca
     1201 actggcgaaa agtctgaccg agtataatcg ggtctatcaa tctgcattct caccgaactt
     1261 actcgaacgc ccacgtctgg aaagccatct acaaaaactc ctgaccgatg ccgtgaaaat
     1321 gcgtggactg attgcgcccg ccagcaaaga aacccgtatt ccaaaatcga tatatgaagg
     1381 tatccagacc attaaccgca atctggtttg tatgctggag ttgcaaatca atgcatactg
     1441 ggccacgcgc cccagccatt tcgtgttatt gaacgcgcaa aaacttcgtg atacccagca
     1501 catgatgcag caaatactgc tgagccttgt tcatgcgctg tacgaaggta atccgcagcc
     1561 ggtttttgcc aatacggaaa aattgaacga tgctgtggaa gagctgcgtc agttgctcaa
     1621 taaccaccat gacctgaagg ttgtggaaac accaatctat ggttatgtgt ggctgaacat
     1681 ggaaacggcg catcagcttg agttgctatc gaatctgatt tgccgggcct tgcgcaaata
     1741 attcctgaac ttcagaatca tcttgctgct gcttcgattc agcaaggata aagggtatga
     1801 tagtgaaaag ggataaaagc attgtcatct gcggcagcta tgagtaatgt tggccctaac
     1861 gaatagcggt tgcttaaacg aatccgactc tcacattatc aggggtataa aaatggaaac
     1921 taccaagcct tcattccagg acgtactgga atttgttcgt ctgttccgtc gtaagaacaa
     1981 actgcaacgt gaaattcagg acgttgagaa aaagatccgt gacaaccaga agcgcgtcct
     2041 gctgctggac aacctgagcg attacatcaa gccggggatg agcgttgaag caatccaggg
     2101 catcatcgcc agcatgaaag gtgactatga agatcgcgtt gacgattaca tcatcaaaaa
     2161 tgccgagctc tccaaagaac gccgcgatat ctccaaaaag ctgaaagcta tgggcgaaat
     2221 gaaaaacggc gaagcgaagt aattcccgtt ttattcaatg agggttgccc ggcaaccctc
     2281 attgctcatt gattcttatc tgtgtatcac cgtcatcatt ctcatccgag aaccaatcga
     2341 aattaacaac agccttcttc tgtatgcagc aaggcaaaaa gttctgtaac tccattgtta
     2401 ttaactgcac tggttactaa cacgttgtgc gctccagctt cccgtaacca acttttcacc
     2461 aaagatattt gttccatgct ggctaaatct gctttggtta ctactccaat gaccgggtga
     2521 ttcatggccc ggtagggcgt ttttacctct aattcctgct tcagcgctga gattcctgct
     2581 tgttgccaga aaagcaggca cctgccgtct gaacggtatc gatccggaag cgtatctgcg
     2641 ccatattctg agcatactgc cggaatggcc ctccaaccgt gttgacgaac tcctgccatg
     2701 gaacgtagtt ctcaccaata aataagcgtc aatacggtgc tccgttgacg cttactttaa
     2761 tccgttttct gagcttagat ttttctgttc ctacccagac cgtggatttg gaattcattt
     2821 tttgtcaggt caatgctaat cgttaaaacg gccataacgc acctccactg gaaggtttat
     2881 cagtaatatc ctaccgttgc ttcatggtcg gggcgtccat tccattatta tcggttttcc
     2941 tttctgttta taaatgaaaa cgccagctgt attcaggctg gcgtcaggag aaatgaagcc
     3001 tgttgagtga gattcaccgg ttctggtgca gaagctgcag atggcgcagg gtaagaacat
     3061 cttctcttgc ctgcttaata caggacaaca gtgctgtttc tgtggttaat ggcactatac
     3121 cctgtatcac tgcattgtct tacagcaact ggtgagggct ggcataagtt gccggtgttt
     3181 cggtagagtc acggtcatcc accacgcgga gtgaataacc gttttcagcg gccttattaa
     3241 taacaccatc aatgtcaacg acaatgtgcc ccatatccag cgcctgtacg ttaacgctgt
     3301 cggcttccag tgtcatcatt tcgcctccgg atacttaccc agggtaatgt tatttaccgt
     3361 tctgtaattg tcgcgggtca tcaggccggt cgccctgcga gcccggagga tatcgatgct
     3421 gtttattaac tgagagcggg tacaggcgct gaatcccggc tggtcggtac gcaccagcgc
     3481 gtatttttcc acgagaaagt tcaccgcatc acacagtgaa atgccagctt caatatgctg
     3541 ctcaatcaca cgttcatcgg cgaacggtgt gtcattcagt gtaaggccgt aatgctggcc
     3601 cagtagtcgg gagagcagag tctgccagat ggtgacggga gacgggcagc tcgatgcctc
     3661 ccgtacgtgt gtatcgggta atgttttcat actgaaggtt ttcctgatat gcagagatga
     3721 aaaatggaaa agtggcgtgg tgaaagcacc aggccggagc agaaggttat tccggtggtt
     3781 tactttttcg tttcgggcgt cggataaaca gccagataaa cgtaaccacc gctaccgaga
     3841 gtgtcagctt tgcaggtcag accttttgca tacagcgtga cggtatgctg atggcgggga
     3901 ttcagttcac cgctggtgag catgagttcc agttgtttca tcagcagcgg aaaggcctgg
     3961 tccaggtggt acgcatctgt atcgctgaac aggcctctga taccggcgcg gtcggcaagg
     4021 taatgcaacc ggttaccctc ctgcaccaga cgtgccccga aacagggcgt cacggtgcag
     4081 ggcagccccc accagggggg cgtgattgtc gtcgggaagt gttgtcccgg ggagtgtgtc
     4141 tgacacgata aaatccctac agaaaatcgg ttaagaatgg tccggtagtg gcgataattc
     4201 tgctcatcag aatgccctga ctcagttcag ggtgacgctc atcagccgga catacgggcc
     4261 aaaactgtcc ttacgacgtt cagcaaacac ggccagcaca ccgggaatat cctgcacttc
     4321 acgaccggta tacgcttcag tgccaccgtg ccagcggtat ttgccggtac agaacggaaa
     4381 aagacgggat gtcggatgtt gttggtgaat acgcatggct tcaccacggg tgatgatttt
     4441 catagtgtga tacctctgaa gacagaagat aaaagtgaaa acaggtgtga tgtggttgtg
     4501 acggtgacgg gttaaagcag accgtgttcc gcaaaggaga aaacctggct gccaccgact
     4561 atcagatggt ccggcacccg gatatcaacc agggccagcg cctgtaccag acgttccgtg
     4621 ataaggcggt ctgccttact gggggtgact tcaccggacg ggtgattgtg cgccagcacc
     4681 acggcagcgg cattgtggta cagggcgcgt ttaatcactt cccggggatg gacttccgtg
     4741 cggttgatgg tgccggtgaa gagggtttca ccggcaatca actgattctg gttattcaga
     4801 tacagcaccc ggaactcttc acgctccagt cccgccatgt tcagaatcag ccattcccgt
     4861 gccgcacggg tggaggtgaa ggccacaccg ggttcatgaa gatggcggtc cagggttttc
     4921 agagcccgca gaatgagact gcgctcgccg ggcgtcattt ctccgggcag aaaggaaagc
     4981 tgctgcattg tccctgcctc cattcagtcg atgatgcgca taatggcgct gcattccgga
     5041 tgctgcaggg cgtaatcccg cagccggtaa tagtggaccg tcatggcata acactccgta
     5101 cgacaggcat gatgactgta cgtcatcaga caggcagcaa tgccagcggc ttccgggctc
     5161 atttcagcgc ggttgccgtt catggcattg aacagtaccc atgtttcgtc atcatcgtca
     5221 tccggttcgg gggccataaa tgccccgccg ttgttcagag tgtacagatt ccagatacca
     5281 ccgcagtagt cttcgcacag acggtccatc cagccgaaaa cacggggctc cagggtcacc
     5341 cactgtggaa tgaggccaaa atgctgcggc cagaagctga tgcgctgttc atcagggact
     5401 atggtggcaa ccagctgagg ctggtcattc cctgatgcag cggttacgga aacagaagat
     5461 gtggtggaat tatgtgaaat ggttttcatg aaatgactcc ttaaaggaca aataaatgcg
     5521 tggttttcag ggtaatggct gaatcagcgc aggctctcca gcagggtttc cgccatcacc
     5581 cacaatgcgc ggttgagctt aatgtcggtg tcgatgctgt gaatggcacg ggtatggata
     5641 cgttttcctt tagcgctgcg acctgaaatt ccgcctttca gcatattctc ctgaatggtc
     5701 tgataagcac tccacaggtc cttaccgtaa tcctcccggc gtcgtggcgt cagaatgtcg
     5761 gcggtggtga cgggctgatg ttcgtcacca taacggtaag tcagtgccgc ctgtgccagc
     5821 gcctggcgtg ccggtggcgg cagaatcagc gactgcatgg catcacgctt ttcctcaatc
     5881 cggtcaaaaa cctccaccac ctcgtaagcc ccttcaatga ctctgtccac cacatttccc
     5941 cggtgcggaa cacgcacttc ccccagagac tgaccacaga cgcatccgtt ctggcagacg
     6001 aacctgaagt aacccggcag catctggtag ctggaggtac cgtcatgaga gttgagcaga
     6061 ataatttcag ggacatgttc tccgtttatc tctccggccc gccgcagacg cagcatgtgt
     6121 tttgtgtatc cccggcggcc cgggtcgcgc acacgggtct ggcaggcgaa gaatggctga
     6181 aagccttccc gctgcaggct ttccagtacg gtgatggtgg ggatgtacgt atagttctgg
     6241 cttcgggacg tgtgtttatc ttccccgaaa atccccggca catagtgcat cagttcttcg
     6301 tgtgtcagcg gacggtcacg gcgtatctgg ttcgcataac caaaacgact ggctagtcgc
     6361 ataatttgct ccttatcggt ggttaagatt tactggtgta ataaatgaaa aagccacgtc
     6421 tcccggagaa gacgcggcct gatgggtgaa atgaatgacg tttattgtct gagaagaccg
     6481 tcactggcga gccgcgtatt aagcaccttt ccggcatcgg gttcagaatt ccggcagcgc
     6541 cagcgtcgct gaccttccgc gttgcggata acccgacgga aatgcattcc cggatcatcc
     6601 tggataaaca cattgcggta agcggttatg acagcaaccg cctgcccgta tgagaaagat
     6661 ccttcagcca ggacatactc tgtgtaaccc ggcatatctg ttttctcctg ataaataaaa
     6721 agcctctgcc acgcgttgtg acagaggctg aagcatgaaa ctgacttcag ggatcagtta
     6781 acgttttttc cggaaacggt aatcagcagt ggatggtagt cctggggatt gaaaaccgat
     6841 aacggcagac tgacacgatg gccgttactt tcttcagttg ctttaatgat ttcggttgtg
     6901 gcgacatttt ccacgcactc cgtttccaga aatgcgtctg tggttcgcgt ggcattactg
     6961 tcaccaaaag cttccgtttc catttttctg gtcaccagcg tctgaccata tttgtctttg
     7021 agttgcagag tgatggtgag ggggccaaat ccttcatcgt ttccgccatt atccagccgg
     7081 aactggtaag cacaaatatt tcccgggagc catatcgtat ctgtattgcg tatactgatg
     7141 taacgttgat cctgtgcccg gagtggggca gaccacgtta accccagaat gaaggcggta
     7201 atcatgcagg ttttgaacag gtgaatcatg gtatttacct ctctgagtca tgacgatgac
     7261 actgacaaat caggtgataa agcgtaaaag gcgcagaata gccgttatgc cggtaactcc
     7321 gggggtaatg cttcttccag tcggttaacc atattgccga gatgggatgc atcatattcc
     7381 atgacggggc gttgcctgat gatactgacc accagtggtt tgattaacat attggtcgcg
     7441 gcccgttgtt gtataccggc ggcgaaaatg atctccagag cattctgatc gataacacgg
     7501 gccagattct cgttcagatt ttcaatctca tgtccggtca gcaggaaact gccggcaagc
     7561 catgtcacct gctcctgaat cattgccaga attttgacgc gatcttcttc aaccagcgct
     7621 ccggcaattt ttcctgatac agcggaggca atcattcccc ccagaacacc gccaaccaca
     7681 cgccctgtca gtgcaccaaa tggtcccagt ggactgaaca gtatcccgcc cgccactgac
     7741 ccgacagctc cacctgccac accggaagat gccacggcaa gattcctgat gaactgcgca
     7801 ccggagatcc gtccccgcaa cattttgatc atgtcagggc cggttgtgac agctaccagt
     7861 gccagagatg tcaccagcgt acccttcatt actttgttca gggcactgat atttcctgca
     7921 ccggtcccct tctgaagagc cagtcggaca gtcgggctgg cagtcgaaaa atcaatgtgc
     7981 ttcagcatac cctgaacaac actgagccgg tggagttgct gggttgtgac gtagacagcc
     8041 agtgtgcggg tgaaggtttt tcctgcctgg acagcagctg tctgcagggc agcatcgcga
     8101 tcgccggtgc tgagccagaa gaccgatgcc gtcagggcaa aactgatccc tccggccgcc
     8161 agactgacaa ccgaaccctc ggcaatatca taagtgaccg attcgatggt cccgaaccgg
     8221 gtgatattac gggcctgagt ataagtcaga tgtccccgac gaatcagccg ggacgcttct
     8281 gcgggatcgg ttacaccggg tactttacct tcgcggatct tattcctcat ggtttcaaca
     8341 gcgccggcat actgatcacg ggggacttcc agttgcatgg ggccattatt tcccatgtaa
     8401 cgatactgtc cgttttgtcc gtcgaatgcc gcaccgacgc tacgggctgc agttgaacaa
     8461 tatttcgtct ggatttctgt tccgctgacc agcctgtctg ctccatttct ggcattatta
     8521 tcaccgagga tccgtgcgtt tttcagacgg actctgtcga caatatggtt tccccgttca
     8581 gcggcaaacc catgtccctg ccctccggaa gcaaacattt tctcatttgt actccaggtt
     8641 tccggcagtt tcatgagaat cagaccattc gccgttgcat cggaaataaa gctgttgtaa
     8701 gggcgttcat aaatttcctg acacagtgtt tctgtttcgc gacaaagaga ggagcagtac
     8761 cattccggat ccggcttatg attttccggg gcatgatgta cttctgtcag cccgcaggag
     8821 gcacagatac gaagctcatc cacgtgatgg aatgatacaa aacggcgacc ttctgaaaag
     8881 ggataccagt caccgccgtc caggcaacga tcgagaaaat cgtcattcgc ttccggtgat
     8941 aaattcaggc ggttccggaa gagcgttttc agttcatcag gtgtgaatga caggttctct
     9001 ttcaactgtt cgttaaaccc tgccatgttt ctttcatacc aggcattgtt cagtaaccag
     9061 tttccgtctg ccagttcaaa ttccctcagc tctccccggg aaatccgttg agaaatgagc
     9121 gtcagccaga tgcgctccgt cagtgagtca ggaaggctgt aatcgttttg caaactggtc
     9181 agccacgatt tactgttgag caccggttgt tcttccagtg tctggctgat ggcacaggtc
     9241 agttttttca gcgcctggat ctggctggcc gcccatagca gcgtcactaa tgcagccagc
     9301 cagtacaaca acccgcccca ccacatcagt gacggtgaaa tatcgtactg taacgcgatc
     9361 aatggtgcgc atgcagaaca cgccataagg aaaaaggcag tataaaaaca gcagtaatac
     9421 ccgttaattt ccttgctgat atcttctggt gtgatcccgt tctgctgaat gtttttgatc
     9481 tgcaggggaa cagccagcag accaccggcg agagcggtgg tcagaataaa tttcacagag
     9541 tcaaaggtgt gagcctccag cgcgatatgt ttaccatcaa gtccccacat gaagctgatg
     9601 atcattccgg caatcgtcag aaatacgcct gccagccagc gaatatggaa caaccgggtt
     9661 atgcgttcgg tgatatagcc agccagtaag gccgggccga acagacacag caatatgagc
     9721 cctgccagcg cagaagccgt gctgattaat gctctgaaca aaaggcgaag aatggcaaat
     9781 cctgcgatca gcaggatcag cgcgccgact atctgtaaca tagtcactcc gtgatgaata
     9841 tcatgttatt gttaatgcca gcgaatgtgg cgttgaagat ccgttgcgcc ggtccgggtc
     9901 ccggtcatga tgaccgggac cacagagagg cgatggttct gtcagaaggt cacattcagt
     9961 gtggcctgac cgttataccc ttcagcgctg ctgccgctga cgctgtgggc ataaccggcc
    10021 tgaacgccca gggtgatatt ttcccggaca cgggcttcca gtccggcctg caggtccagt
    10081 gatgtgccat tctgtgaggg agagaacgtc atcccgctgc ctgcagtgga agtccccaca
    10141 cgcatatctc cccgggagct gaaggtgcgg ataacagaag gctgtaccca ccagttcacc
    10201 ggcagttcac gcacactgtg ttttgcactg tcacgcaggg gggcacggga tgaggtgcct
    10261 tcgccaaagg tcatatcgtt gtggctgccc agacggaaac cggcacgcac atgttgtgca
    10321 ctgccatgcc cgaacttcac ataaccggcg ttgtccttac cgtcatccag ggaaagtccc
    10381 tgccaggtat actgcagttg tggctccagc atcaggttgt cagtgatact gaagggcaga
    10441 ccggtttcca gtgagcccag ccagccccag ccccgggcgc ggaagtcgtt attgtccgat
    10501 gacgctttca tgctgtggcg ggttccctgt gccacaatgt cagcccacag gccggaggac
    10561 gtgtgtgtca gattcaggta tccgcccagg ctgccggcat catcccggac cgtgccggca
    10621 cgggagccgt catcatcctt aacatcaacg gaagaatggc cagcagcacc atataccccc
    10681 gcggtcacag acataccggc aacctctgtt ctcatcaggt caccctccag acggacgaat
    10741 ccatagctgc cgctgctttc cggcgtggcc ccacgggcaa taccgccatt gttatcgtga
    10801 ccgagatgac cgccctgaat gctgagacgg acgctgttgt tttcaccatt tacaccggtc
    10861 tgatggctgc gggagcctgc cacaatccgg tcatagtcca ttgcctgtgt cagcatggag
    10921 gcatacaggg ggacttctgc acgataagca ttttcactgc gcagatacca gctctcatca
    10981 ctgtcccggt tgagggagta gttaaaggca ccggcctgca gcctgttccc ctggacaaag
    11041 gccccttcct ccgtggtggc accgttaatg gcttcaacca cctgaatacc cttaccgctg
    11101 gtcgccagcc ccgacgcact gttgccggcg ttcaccaggt tcaggatggt ttttccggtt
    11161 gccctgccgc cgtcaatgac cagtctgtca gcattgttct gtgccatatc cgggcgtaca
    11221 cgcaggctga tggtgccatt ctgtccgttc aggtttttca ctttcagggt tgccggtacg
    11281 aacttccctg tgcgggtgga ggtgaaatga atctgtccgg catggctgag gtcatccacc
    11341 accgactgca ccgtggcgtt atcggggata ttccaggtgg caccggaggc gagagtgaca
    11401 ttcgtggggt caatggcacc gttcagcaca gtgctgccgg tcagcttcag ggctgtaccg
    11461 cgctgagcaa tgacatccgt ggtgacggta ctgtcgttca gcgtcagcgt gccttcattc
    11521 aggttgacgg ctttctgggt gagtgtggtg ttgctgacag tgagtgtgcc acttcctgat
    11581 ttttccacgc tgccgttacc ggtgagagag cctccctgca ggagtgcatc gccttcacgg
    11641 atggtcaggg tatcgttgtt caccgtcttc ccggaaaggg taagtatggc gccgttattc
    11701 agcgtggtgg tgcccgccag tgtgccgccc ctggcggtga acagtccgcc atttaccgtg
    11761 gtatccgtgg ccgtatcacc ggcgttcagc gtgaatgaac tgcctttttc cagcatcagg
    11821 gcatccgcct gaccgcctcc gatactgaat gcctttccgt cgctccgggt accactgaca
    11881 gcggcaccgg aatcggccag cagtacaccg ccatttccca tggatacggt ggtggcggtg
    11941 ccaccgttgc ggacatccag cgttccgcca tcatccacgc gggtattagt ggctgtgtgt
    12001 ccggtcagca catccaggcg tccgccattt tccagtacga cattatcagc tttaccctcc
    12061 acaacagaga atgctcccag gcggtttatg ccggtaacgg ttgcagccgt actggtaacc
    12121 agtgcgccgc cctgcttcag ggtgacattc gtggctgtac caccggcgtc cacctgcagt
    12181 ctgcccttct gattaacggt ggtattcccg gccacacccc cgtttttgac aatctgccag
    12241 ccgtcactgt tcacaacagt gtcagacgct gtaccgccgt tgtgcacata ctggtatccc
    12301 ccattcagcg tggtatccag tgcgtgacca tgtacagtct ggtcgccgcc ggcataaacc
    12361 acagtggtat ttgccgttcc ttcagttctc acaatctggc gaccgttttt attgatggtt
    12421 gtgcgtacgg catccccgcg aacaaactgc ccggtatcac cgttttctgc atccggtccc
    12481 ccttccgccc cggtattaac aacggtatcc gttgccactg taccgggctt gacgacctgc
    12541 cagcccttat cattaatgac ggttcctgtg gctatcgccc cctcatgcat ccactgttcg
    12601 ccaccattca gcgtggtgtt cactgcccgt ccctgaaggc tctgtccgcc tccggcactg
    12661 ataaccgtgt ctgagacact tccaccgggg ttcactctct gaagaccacc actggtgaca
    12721 gtcgttttgt tggctgttcc gccatcctgt acccattgcc cgccggtatt ggcctcgtta
    12781 tccggcccat actccagccc ggtactgatg gtcattccgt tggtcgtacc gaagacaatc
    12841 tggttgtcat gatttgccag tgttccgccg ttcacggttt ctcccgggtg cacaacgatg
    12901 tcagcagcca gcaccgggag tgacgtgact gcggcaagag acagtgcaac cgccacaccg
    12961 ccacgtttac cccgtgcgcg ggccagttcg gaggcaacca cgaaagcgcc cgtcatgtga
    13021 ttccatacca gcctgtagca ggtattcaga tgtcgtttca tcagcttttc cttagattga
    13081 gggtgaataa aaaagccggt acccacaaac gtggataccg gcaggtaaag ggttacagtg
    13141 ccggaggtcc ggctgtggga gtttctgaat tgtggttctg ttgttccctg aatgtctttc
    13201 tgtacgtatc tgtgcatgtt actgacaaag gttattatta gcttatcgat cgatataaac
    13261 gatcgataaa atagatattg atcgttttat tctattattg agctattgca cggacaagag
    13321 agacagtaac gaaaacagtg cttttgtgag cagactcaga gtcagggagg gcatcagtca
    13381 cgacaggttc agaagaaaat ccagttccat accgcacggg caacagagac caccgtgtca
    13441 cgaacagcgc gcagggccgt acggacaaca gaggcaacac agacgctctc cgctgtgtca
    13501 aatatccggt ccaccgcacc ggtaaactgt tcacgggcct gagagcggac agattccgtg
    13561 cgcagctcgt cctgcagtcg ggtcatcagg ggactggcgg catggtcggg aagcgctgtc
    13621 atgagcgcac tgaccagcgt atccagttcc cagccggtgc gggccgatac ggccacaacc
    13681 ggatgtacgg gccggaacag acggaatacc gcctccgttt tttcgcgaat attctgtgcc
    13741 tgtgcaggag aaggctgaat accggccata tcccattcat ggcagggctc cgttttgtcg
    13801 gcctgcgtca ccacaaacag cacctgctga tgtccgcact gcaggatgtg tcgccagaaa
    13861 tactcatcca cagacagggc acggtcatcg gctttaatca gccacagtac caggtcaagt
    13921 tcaggcagaa tgtcacggta cagggcctca tactctgcat ccctgtcccg gctctcgccc
    13981 accccgggca ggtcagtgat aaccatgctg tgaccgtggc cactcagacg gaagcgccgt
    14041 acttcccggg tgccggcgtg aacatcactg accggggtga cctccccctg aaacagtgca
    14101 ttacagagtg aggatttacc ggccccgctt ttacccataa tgccaatcac gggttcgtga
    14161 cgggtgagtt tgcgcagatg ttccaggatg tgacgggaaa gcgagtaagg cagggaggag
    14221 agcggttttt caattgcctc aatggcatca gacggattca tacatgtatt cccggtgaaa
    14281 aaagacaaaa ccccgcgcac cggaaaggtg gcgggggatt cacggagata atatctgtct
    14341 gaaattgttt tgaatcgatg ggattgttgc gtattaacat gaaggagatc aggacttatc
    14401 cgttactggg tgatctcctt ccatgaaaat acgccgttat tgttaaacag caggctgatt
    14461 tctccgtaaa ggggaaaata tttatcggct gcatgattat caggtgtgtc tgtgtttact
    14521 gctgtgagta tttttcagtg tattgtgggg gagatgtcag tgggagatat taaatttacg
    14581 tttataatta tcttacttct tctgtgtgtt gtttataaga gtgcacacgt aatgatatct
    14641 gttgatacca tgtggttaag tgtttccctt cctggtcatg cgtaatgatt aaatgctttt
    14701 gtgacaccat tcttccggtc tgaatattta tcccgttctt ttttatttgt gcagtgatac
    14761 tgtcggaaga gaattcagta atgtgaggtg tcctggtcag gataatatct gctgagatat
    14821 tattagttga gtgtgttttt actgagcaaa agctggcttt taattcctgt actgaaacac
    14881 tgtcgctcgt ccggggttaa ctgaaactgg atgatgatgt aaaatgaagg ctgtttttgt
    14941 gtttgtcgaa gaagtgttat tttaatccat tctcatcatt ggctggcgat tatacagaag
    15001 tatttgaaga aaaaatggtg cattttatct gtgtcagcag ggaggaagta ttttattgat
    15061 gtatcagtat taaatggttt ttgttattta taaaaatgtc atcctgtcca tagaatattg
    15121 tactcagcat tcagtgcagg aactgtagtc ttctttattt tattcttctt caggaggggg
    15181 ggtgattcct gaaggcgtta ttcccggtgt tctgtttatg attcttcccg gcaggaaacc
    15241 ggtatctgca atacccggga tgatgctttt tgttcgtacg tggttgtgta aaatttgaat
    15301 agtggatgtg ctctgaaggg aaggagggga cacacagatt cattttgtct ttcttcagca
    15361 gcaaaacaca ctgtggttat tatttaacca ttaaatcaac aaaaattttt acctcatatt
    15421 gatttgactg ctatttttgc tattaatagc cagaattgca gtgcttttat atctgtgtgt
    15481 agtgaggtta ctatgtcatt cggtcagcct tgtgatgagt ttcccctgag tgcgctgcct
    15541 ccactcattc gtgatgccgt tattgaagct cagcaaatta cgcaggctcc ccttgggctt
    15601 gtggcagcgt ctgccctggg cgctgtttca cttgtctgcc agaatctgat tgatgtctgc
    15661 agactgaata cactgcgggg gccggtctct ctttttttcc tgacgcttgc cgagtccgga
    15721 gaacgaaaaa ccgcagtgga caaattatta atgaagcccc tgtaccagca ggagatgcag
    15781 ttatattcca gatacaaaag tgaactggct gtctggaaaa acaaggaaga gcttttaaag
    15841 gctcagaaaa aagcattgtt gtcaaaactg aataaagaac tccgcaaagg cgcggatgag
    15901 tcagaaaccc tcagacaact ggaagtactt cagaaaaaca gtgcggaaga acctgtgagg
    15961 tataaattca tttttaatga tgccacgact gcggcaatta agaatcagct ttgtggtaaa
    16021 tggcgttcag tgggtattat gtcagatgaa gccggtatta tatttgatgg atataccctg
    16081 tctgagttgc cttttattaa caaaatgtgg gatggctctg tattgtctgt ggacagaaag
    16141 aatgaacccg aacagatgat tgaaaatgcc agaatgacgt tatctctcat ggtgcaaccc
    16201 ggtctttttg accgatatat ggagcgaaaa ggttctgttg ccagggacag tggttttctt
    16261 gcccgttgtc ttatcagtaa acctgcaacc acgcagggga aacgctttat taacggagct
    16321 gtgattccgg gaggatcatt aacggcattt catgaacgat taatggaact ggccagaggc
    16381 agtattgaaa aatccagtga ggatgaacgg tattgtctgc atttttcgcc tgaagcgcaa
    16441 aaaatattta ttgagcacta taatgttctg gagcaggatt tatccccttc cggaccctta
    16501 agcccgtttc ggggacatgt ttcaaaaaaa acagaaaaca ttgcaagaat tgcagcgtta
    16561 tttcagtatt tcagctatgg tgaagggaaa atctctgccg atatcatgac gtctgctgtc
    16621 gtaataagtt tgtggtacac agatgaatat aaaaaattat ttgcgttacc tgatgaatct
    16681 gaactgcagc agaaggacgc cgaggagctg ttcgactggc tcattgagga gtgccgcgga
    16741 gaatgcccac cccgtgtcag gaaaaattat attctccagt gtggcccggg ccggttcaga
    16801 aacaggaaaa aactgaacgc attgctgaac attcttgaat ctcagttcag gctttctgtc
    16861 gtgccggaag gaaaaaccat gtatgttctg cttccccaga tagccagttt aaagttgtct
    16921 gatgtctctg gtatttttac ctcgggttat cactacaata agctgagagc gaagtaaccg
    16981 gtgagcagtt tttctccatt atgcttcaga cttagatgat tgattattat ggggatttca
    17041 tcaccgatat tcagatgtat gaatatcggt gatgaactaa agctcaggag ttgcgtggtg
    17101 gtgatgaatc aacaggcata taactgatac ccgccggtca gaagttccag cctgcgactt
    17161 tcctgtctgc gtgcccgcag tccggcgttc aggtgcagcg tgtgacagaa cgcacggaga
    17221 gcgaaacggc aggcagacag gccgttcagt cagccctggc accctgcccg gataccagct
    17281 tccctgaaac aactggcatc aggtgcgctt atcgtcaacc gttgtgcggt atgccaggag
    17341 cacgtactga tccatcagat ccgtcagtgc tgcgctctgc ttctgacggt tgatgatcca
    17401 ttcctccagc tctttttctg atttccgttt cagattctcc gtccagtcgt caaggcagtt
    17461 tccggtcatt aaatcaagcc agcaaagcca gaccagtttg aggcgcaact ccgtgccacc
    17521 aaactgacgg aagaggctga tcagtttatt tgccagcgaa cgctgcctca gacgttcaca
    17581 ttcagacagg agaatgcctt ccagcatcgg atgatgaacc agcagatgtt cactggtgca
    17641 taaattcact atcagatcat catccagggg cggcgtggtc cgtttgtccg gaccataaat
    17701 cagccggtgc agactctggg gtacgatggc aatctccggt ttttcaccat tgaatgacac
    17761 ccacccgggc gtcagattat ggaagtagta gatattcgga tgacacacgc cccaggatac
    17821 ggcgtgccgg gtgaagccgg tcagccattt ctggtagctt tcgtggagca gctcgtaacg
    17881 ggtctgatac tgtggtttca ttttcatatt cctcacgggg gataacaggc agcagaacgc
    17941 cgccggcagg ccggagactg cgtctgctgc agatgaacgg ttaagaatta cggacgggac
    18001 tgtgcaatac gcgcctgcag ccaggcttcc acttcgcttt tcagccatcg ggagctgcgg
    18061 cccattttga tgggcgcagg aaagtcacca tccttgatga gtttgtaaaa ccacttatcg
    18121 gtcaggccgg tcagttgagt gataaatgcc atgtcgacca tctggtcatc catcagtgaa
    18181 actggggtga tcatgagaat gttcctccgg atgttgttaa accagccggt gtggtcatga
    18241 aaacaacgca ccaccttcac cggcataaca accggggaga aacgtaaaaa ataatcggga
    18301 atgtgaccgc caggagataa acggggaggt tacggtggct gagccgtacc ggtatgccgg
    18361 gtggttgtta cgaaacgatg cttccgcacg cgcgcgcgat aaagaaggga tgatttgcgt
    18421 gaggaagagg ggaaaactgg cgttctgata attgcagagt tatttacggg gcggttacga
    18481 tggggcagac cgtgtccggt gggcaggcga caggtatcac cgatgggata agactgtctg
    18541 attggtatta taaatatata agagagatca aacacatgag atcaaataca tgagttcata
    18601 tatgtgaaat aaacacggtg agtgtgatca gtgggcatga tgagaacgca gtagcaacag
    18661 actgcgctat ccctgatagc tggcgtataa cccgcgtata taaaacgtat ctgcaatgta
    18721 cgtgaacaga gtgcattaag catccgtctg agcggtaaac atatccgacc tggtgagtga
    18781 cagtctgaga gaccggttaa gtggtggctc gggagtaatg agtttggggt gggtaccaga
    18841 atgccagtct ggcggtatgc tgacagtatc acgttaattg cagcataaag tgatgagagg
    18901 ctaatggaag ttaaaaccag agcagttccg tcgttgttca tcccgggaga cgctggaaaa
    18961 agtgatttcc cacacgcgtt ataagcttac tccagcggag atggaagcct tcaactctgc
    19021 ggtcgatcac cgactggcag aactgacaat gaacaagctt tacgatcgtg ttcccgcttc
    19081 agtctggaaa tatgtcatct gagcgttcgg taatcacaac tgcatcgcgc caggtgttat
    19141 ccgctttgtg attcttattg gctcttctca ggttattaaa agctgaatat gttcctgttg
    19201 tcgggaacca ggataatacc gggagcgtta agcggtatag cgacaacaaa gaattcgaaa
    19261 tcaaaaggga atggtgccgg aggcgtgggc ggtaataggc tgattttcag gtattaccgg
    19321 cttacaacgt gtggttgata ttgaggggct gtgagtttcg cgtatttgtc agaaacccag
    19381 atgagttttc ctgtatggga cgaaataagg cagcggcaat acctggaaaa tttaatatgg
    19441 tatgaaggag aacctgtcgg ttacgcttaa tatacacggt acactgtcag ctcaggggat
    19501 acatgaaccg ttatctcacc agctacacag tatacacaca ctgacgctga cagtagtcag
    19561 caggaccaca ataatcagcg ccagcagtat ggtaacgata cggtctgtat tccggtagta
    19621 gccgaagggg tcgacagaac cacaatacgg gcaacacact gtattattag taatcgcgtt
    19681 tccacagtcc ctgcacttct ttttgcgtat caccataact ccctcctcac agccttaccc
    19741 gtaaccggtt ttttacatta aaaaacaacg ctgaaaattc aaccgtcagt ccggagacga
    19801 ccgttcgggt tatcacagag tgcctgagac agtgtcctgc cggaggtcac ttcagggact
    19861 ctgcgcattt tttacgacgt ggctattccg tatgaaccat acggagattt aaccatgacc
    19921 tacaaataca accccttctg gcagcaacgt attcgtgaga cggtgcggca cgcactgaat
    19981 gttcatcccc gcctgacggc attgcgggtt gacctgcgtc tcccggatgt accggcagca
    20041 acggacgcag ctgtgatatc ccgcttcatc aatgccctga aagcccgaat cgacgcttac
    20101 cagaaacgta agcatcggga aggtaaacgc gtgcatccca ccaccctgca ttacgtctgg
    20161 gcccgggagt ttggggagtt caaaggtaaa aaacactatc acctgatgct gctggtcaac
    20221 cgggatacct ggtgtcgtgc cggtgattac cgcgctccgg agtctctggc cgggatgatt
    20281 aaacaggcgt ggtgcagtgc cctgggagtg gatgtcgggt gccatgccac gctggtgcat
    20341 tttccggcct ggccggcggt gtggctggcg cgtaatgatg acaccggctt tcagcaggtg
    20401 ctggaacgtg ctaactatct ggcgaaggag cataccaaag ctcactgcac cggtgagcgc
    20461 aactttggct gcagtcgtgg ctgagccaga cagacgagtt atctgacttc agtgtgatga
    20521 cgtgttcacg gccaccacac tgattttccc ttatgagact gaactgaaac cgccattcac
    20581 acc
//
LOCUS       DAFNBJ010000084        20211 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000084 DAFNBJ010000000
VERSION     DAFNBJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.084"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(83..295)
                     /gene="cspA"
                     /locus_tag="L2X49_002903"
     CDS             complement(83..295)
                     /gene="cspA"
                     /locus_tag="L2X49_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBN4797965.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(576..866)
                     /locus_tag="L2X49_002904"
     CDS             complement(576..866)
                     /locus_tag="L2X49_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBN4797966.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1300..2010
                     /locus_tag="L2X49_002905"
     CDS             1300..2010
                     /locus_tag="L2X49_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBN4797967.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2060..3034)
                     /gene="ghrB"
                     /locus_tag="L2X49_002906"
                     /pseudo
     CDS             complement(2060..3034)
                     /gene="ghrB"
                     /locus_tag="L2X49_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
     gene            complement(3138..3797)
                     /locus_tag="L2X49_002907"
     CDS             complement(3138..3797)
                     /locus_tag="L2X49_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBN4797968.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3950..6283
                     /gene="bisC"
                     /locus_tag="L2X49_002908"
     CDS             3950..6283
                     /gene="bisC"
                     /locus_tag="L2X49_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBN4797969.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGKTVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYIGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6252..6692)
                     /locus_tag="L2X49_002909"
     CDS             complement(6252..6692)
                     /locus_tag="L2X49_002909"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBN4797970.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6689..7252)
                     /gene="tag"
                     /locus_tag="L2X49_002910"
     CDS             complement(6689..7252)
                     /gene="tag"
                     /locus_tag="L2X49_002910"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBN4797971.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7410..8108
                     /locus_tag="L2X49_002911"
     CDS             7410..8108
                     /locus_tag="L2X49_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBN4797972.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8337..9545
                     /locus_tag="L2X49_002912"
     CDS             8337..9545
                     /locus_tag="L2X49_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4797973.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9869..11560
                     /gene="eptB"
                     /locus_tag="L2X49_002913"
     CDS             9869..11560
                     /gene="eptB"
                     /locus_tag="L2X49_002913"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBN4797974.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11652..11728
                     /locus_tag="L2X49_002914"
     tRNA            11652..11728
                     /locus_tag="L2X49_002914"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11686..11688,aa:Pro,seq:cgg)
     gene            11763..11896
                     /locus_tag="L2X49_002915"
     ncRNA           11763..11896
                     /ncRNA_class="other"
                     /locus_tag="L2X49_002915"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            12642..14249
                     /gene="dppA"
                     /locus_tag="L2X49_002916"
     CDS             12642..14249
                     /gene="dppA"
                     /locus_tag="L2X49_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBN4797975.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14557..15576
                     /gene="dppB"
                     /locus_tag="L2X49_002917"
     CDS             14557..15576
                     /gene="dppB"
                     /locus_tag="L2X49_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBN4797976.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15586..16488
                     /gene="dppC"
                     /locus_tag="L2X49_002918"
     CDS             15586..16488
                     /gene="dppC"
                     /locus_tag="L2X49_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBN4797977.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16499..17482
                     /gene="dppD"
                     /locus_tag="L2X49_002919"
     CDS             16499..17482
                     /gene="dppD"
                     /locus_tag="L2X49_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBN4797978.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17479..18483
                     /gene="dppF"
                     /locus_tag="L2X49_002920"
     CDS             17479..18483
                     /gene="dppF"
                     /locus_tag="L2X49_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBN4797979.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18513..19784)
                     /locus_tag="L2X49_002921"
     CDS             complement(18513..19784)
                     /locus_tag="L2X49_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBN4797980.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4823 a         5036 c         5340 g         5012 t
ORIGIN      
        1 cgattattta tttcctgaaa aacaaataaa aaaatccccg ccaaatggca gggatcttag
       61 attctgtgct tttaagcaga gattacaggc tggttacgtt accagctgcc gggcctttag
      121 cgccgctttc gatggtgaag gacactttct gaccttcgtc cagagatttg taaccatcgt
      181 tctggatagc agagaagtgt acgaacacgt ctttagagcc atcgtcagga gtgatgaagc
      241 cgaagccttt gtcagcgttg aaccatttta cgataccagt cattttaccg gacatagtgt
      301 attaccttta ataattaagt gtgcctttcg gcgatatggc gtgctttaca gattttgaag
      361 cgttaaagga atgtgcacta cgaggggtat caacgataac tcttgaaggg acttgcctta
      421 ctacactgct ttaatggtct gtacgtcaaa ccgttgatgt gcattaagcc acgcattggc
      481 gggtgatgca acaattattt ttcatattta tgattaatcg gttggaaaac ggtcctgtca
      541 tcaggaccgt aaacagcaat aaagtggata aaagtctatt ccatcaactg cttacttaat
      601 gccgggttgg cttgaatcaa acgcatcaat tttagttcgg cacttgaagg cttcacgcgt
      661 ctggattccc attcctttac catggcgact gatacgccca aaacccgggc gaaatcatcg
      721 atttttaacc ctgtcccttt tcgtaactgc tcaatttcgg tacaggacgt tgttctgtgc
      781 gtcagggtaa ttttctgcgt ttcatcttta aaaacaatct gttccaggct gctcaacagc
      841 tcatgcattg gatctttata ttccattgaa aactcctcaa atcacactgc gggatcgtga
      901 attgcatcga agctcattaa gaatagtcgg gaaaagcaga cacagcgggg tgaagtcagg
      961 gcatgctctg gctatgattc gattcagcca gtcatttagc gtgacttata gccacctaac
     1021 gatctgataa atgccgatat atctcttacc actcctggtt tgtaaagatt tcgaaaatct
     1081 aaccttctgc gaaggcgtaa atctctggct acaaaaaaag caacggcact atcatttttt
     1141 aacaatgaaa caacaaatta accttgtttc attttctcca tgattttacc aggaagatta
     1201 cgtagaaagg gtatcctgca agcctgtcct ggactatcct tgtcacgtca gacacgcgtg
     1261 tgtcgttgtg cgcttttttt gggtgaaagg agtaagaaaa tggcgacagg aaagtcctgc
     1321 tctcgctggt ttgcgcctct tgcggcgtta ttaatggtag ttagcctgag tgggtgtttt
     1381 gataaagaag gcgatcagcg taaagcgttt atcgacttcc tgcagaatac agtgatgcgt
     1441 agcggtgaac gtctaccaac cctgactgcc gatcagaaaa aacagtttgg tccttttgtc
     1501 tctgattacg cgattctgta tggttattct cagcaggtga atcaggcgat ggattccggt
     1561 ctgcgcccgg tcgtagacag cgttaacgca attcgcgtac cgcaggatta cgttacgcaa
     1621 agcggtccgc tgcgtgaaat gaacggttcg ctgggtgtgc tggcgcaaca actgcaaaat
     1681 gcgaagttgc aggccgatgc tgcgcactct gcgctaaaac agaccgatga tttgaaaccg
     1741 gtctttgatc aggcattcac caaagtggta acaacgccag ctgatgcatt gcaaccgttg
     1801 attccggctg cacaaacttt cacgcaacaa ctggtgatgg ttggagacta cattgctcaa
     1861 caaggtactc aggtgagctt tgtagcaaat ggtatccagt tcccgacttc gcagcaggca
     1921 agtgaatata acaaactgat tgcgccgtta ccggcacaac atcaggcgtt taatcaggcc
     1981 tggactacag cagttactgc aacccaataa agagtaaaag cccgagcgat tatcgggctt
     2041 tacttcacgc agtcgcggct tagtccgcga cgtgcggatt cacacagttc ttctcaacct
     2101 ttccttgtaa cgcatcaatc aaattatcca cggcacaggc ggccatgcca taacgcgtct
     2161 catgggtggc agatccaata tgcggtactg cgacgacgtt ggccattgag agcaacggcg
     2221 aatctacgga cagtggctct tgttcgaaga catccagccc ggcggcgtga atttcgcctt
     2281 tctgcaatgc agcaatcagt gcattttcat caaccaccgg gccacgtccg gcattaatga
     2341 aaatggcgga ggatttcatt ttggcgaatt gttctgcgcc aaacagatga tgcgtctcat
     2401 cagttaacgg caggatcagg caaacgaaat ctgactcttg taacagagta tccaaatcgc
     2461 agtagcgggc gttgaagcgt tcttctgctt ctttatggtg gcggcgcgcg ttatagagga
     2521 tgggcatgtt gaagccaaag tgcgcacgtt gtgccagcgc catgccgatc cgtcccatcc
     2581 cgacaatgcc cagtgtttta tggtgaacgt cagtgccgta ccagtccggg cctatgctcg
     2641 cggtccattc gcctgctttt acccgttctg ctacctccac aacccgacga gcggtagaca
     2701 acaccagcgc catcagcgta tcggcgacgg tttctgttaa tacggttggc gtgtgcatca
     2761 gcagaatttt tcgggcggta agcgcatcga catcaaaatt gtcatagccg acggagatcg
     2821 ttgatgtggc acgcagtttc ggcatttttt ccagcaatgc ggcatctaca ttctcgtttg
     2881 aacccagtaa accttcagct taggcaaaaa ttgctgcatt ttgttcgacg gtttgtgggc
     2941 tgaggtttgc cacctggtga acggtgaaat gctcttgcag gcgttgcagt aaatcatcag
     3001 gtaaggcttt gtagaggata acggacggct tcatgcttct ctccattcac tgataaggcg
     3061 atcaccttag cctgaaatat gatgaaggaa aactgaagaa tgccaggtga tttctgcatc
     3121 acctggcatg aaagggatta cagcgggctt aaggtaattt ctacacggcg gttttgcgcc
     3181 ttaccttctg cggtgctgtt gctggcgatt gggttagccg ggccaaggcc ctgagtacgg
     3241 atgcggctgg cgtccacgcc ctgggtgatc aacgcgctgg caacggaatc cgcacgttgc
     3301 tgggagagac gcatgttcag gtcgtgacca cccgtgctgt cggtataacc aatcacgtta
     3361 accgccgttt tcggatactc tttcagcacc attgccacgc cggtcagggt gttagcgcct
     3421 gccggtttca gggtcgcgct gctgctgtcg aaggtcacat tgttcggcat attgaggata
     3481 atgttatccc cgctgcgggt tacgctaaca ccagtgccgc gcattttgtc acgcagcttc
     3541 gcttcctgca catccatgta ataacccacg ccgccgccca gagctgcgcc tgctgctgcg
     3601 ccaatcagcg cgcctttacc gcgatctttc ttcgaagaag agagcgcacc aatacccgcg
     3661 cccacgagag agcccagacc tgcgccgata gcagatttgc ctgcttcgcg ttcgccggtg
     3721 taagggttag ttgtgcagcc agataccgcc agagcgccac tcactacggc ggcaataaga
     3781 taaacacgtt tcttcattgt taatccttaa taaccttttt attctttgcc acgggttccg
     3841 tggcgggaga ttatgccgcg tgaacatgaa gattattcct gggaatactc ggaaatttgt
     3901 aagtaatatt taactgctca atacatctaa cctttcagga gtcttcggtt tggccaactc
     3961 atcctcacga tattccgttc tgactgccgc ccactggggg cccatgctgg ttgaaaccga
     4021 cggcaaaacc gtgtttagct cgcgtggcgc gttagccaca ggaatggaaa actccttgca
     4081 gagcgcggtt cgcgaccagg ttcacagcaa tacgcgggta cgatttccaa tggtgcgcaa
     4141 aggctttctc gcgtcaccgg aaaacccgca aggcattcgt gggcaggatg aatttgttcg
     4201 cgtgagttgg gatgaggcgc tggatcttat tcaccaacaa cataaacgca ttcgtgaggc
     4261 ttatggtccg gcatcgattt ttgctggttc ctacggctgg cgttcaaacg gcgtgctgca
     4321 taaggcctcg acattattac aacgctatat ggcgctggca ggcggttata tcgggcatct
     4381 gggggattat tcgaccggtg cggcacaggc gatcatgccg tatgtcgtgg gtggcagcga
     4441 agtttatcaa cagcaaacca gttggccgct ggtgctggaa catagcgatg tcgtggtgtt
     4501 gtggagtgct aacccactca atacgctgaa aattgcgtgg aatgcatctg atgagcaggg
     4561 gctttcttac ttttctgcac tgcgtgacag tgggaaaaag ctgatctgca ttgatccaat
     4621 gcgatcggaa accgtcgatt tctttggcga taaaatggag tgggtggcac cgcacatggg
     4681 caccgatgtt gcgctgatgc tggggatcgc ccatacgctg gtggaaaatg gttggcacga
     4741 cgaagcgttt ctggcgcgtt gcaccacagg ttatgccgtc ttcgcctctt atttgctggg
     4801 cgagagtgac ggaatagcaa aaaccgccga atgggcagca gagatttgtg gtgttggcgc
     4861 agcgaaaatc cgcgagctgg cggctatttt ccaccaaaat accaccatgc tgatggcagg
     4921 ctggggaatg cagcgccaac agtttggtga gcaaaaacac tggatgatcg tcacgctggc
     4981 agcaatgttg gggcaaatcg gcacacccgg cggcggtttt ggtctttctt accattttgc
     5041 caatggtggt aaccccacgc ggcgttctgc agtgctctct tccatgcagg gcagcttgcc
     5101 gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag cactggaaaa
     5161 ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata ttcgttttat
     5221 ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc tgatccgtgc
     5281 ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg cggcgaaaca
     5341 cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca ccatgaccgg
     5401 tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac gctatgaagc
     5461 gcgtaatgat tttgatgttt ttgccgagtt aagtgaacgc tgggagaagg gcggttatgc
     5521 acgttttacg gaaggaaaaa gtgagctgca atggctggaa acgttttata acgttgcccg
     5581 gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct ggcaagccaa
     5641 ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct tcgctgattt
     5701 ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg aaatcttctc
     5761 acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt ggctggagcc
     5821 ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg cccatccggc
     5881 gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg cggtagcaaa
     5941 ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcattc aagatggcga
     6001 tactgttcgg ctgtggaacg cacgcgggca aattcttgcc ggagcggtca ttagcgaggg
     6061 aattaaacct ggcgtgattt gtattcacga aggggcatgg ccggatctgg atttaaccgc
     6121 tgacggtatt tgtaaaaacg gcgcagtcaa cgtgctgacc aaagatctcc ccagctcgcg
     6181 gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat acaacggtcc
     6241 ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt gggtagttgg
     6301 gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta aaaatctatc
     6361 gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg ctgcacatac
     6421 tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa cattgctgcc
     6481 agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc ttcccagacc
     6541 cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc acgccagtaa
     6601 ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc gaggatcgcg
     6661 gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag caacagcagc
     6721 caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag attgtggtgc
     6781 cgacaaactt aaaaccacgt tttttcagtg ccttagatag ggcgtcggag gcggacgtag
     6841 atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga tttacaaacg
     6901 accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac gcccgcgcat
     6961 taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc tgtaccagtc
     7021 tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga tgaaagcagg
     7081 cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc tgctgccctt
     7141 caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc cattcattat
     7201 catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc atactttccc
     7261 tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg gtgaaaaagt
     7321 caatatttaa tacagggcta ctatgcccga tatccttcat gcaatgacaa taatattgcc
     7381 gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt ggtcgctggc
     7441 agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca gcttacgcct
     7501 ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt tacacctggg
     7561 atagtgatca tcaacctgat tacaacgata ttttgtcgca acgtattcaa agtagccaaa
     7621 gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg accagcagta
     7681 tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg gtcgcggcat
     7741 tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt actaccacgc
     7801 tgaccgatcc gttatggcat gccagcgtga gtagcttagg ctggcgtgtt gactcccggc
     7861 ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc gagaatatct
     7921 ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc aactggctgg
     7981 atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat gccgcgctaa
     8041 ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg gtgagcgcca
     8101 gattttaacg taacagtcac aattgaaacc attgaataac aacagttgtg gcgatagtgg
     8161 gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca ttcctgaact
     8221 aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc ataaaatgca
     8281 cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat actgccatga
     8341 caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc attacccagt
     8401 ttgcactggg gtcggtttat acctggagcc tgtttaatgg cgcgctttct gccaagctgg
     8461 atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg ctggcaattt
     8521 cgtcttctgt tgcgggcaaa ttgcaggaac gttttggcgt taaacgcgtc accatggctt
     8581 ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac ctgatgatgc
     8641 tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac ctgctgacgc
     8701 tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg ttcgctatcg
     8761 gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg ctcgaaacgg
     8821 tgggtttgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg attgttttcg
     8881 gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat ggtgtggtgg
     8941 agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg ttagcggtaa
     9001 tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa gatatcgccc
     9061 aaagtctggc acaccttgat gtggtttccg cagctaatgc agtcactgtt atttccatcg
     9121 ccaacctttc aggtcgtctg gttctgggga tactgtctga caaaatcgcc cgtatccgtg
     9181 ttatcaccat tggtcaggtg atttcgctag tgggtatggc ggccctgctg tttgcaccac
     9241 tgaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt ggcggcacta
     9301 ttaccgtctt tccgtcactg gtcagtgagt tcttcggcct caataacctg gcgaaaaact
     9361 acggtgtgat ttacctcggc ttcggtatcg gtagcatttt tggttcgatt atcgcctcac
     9421 tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt ctgtcattgg
     9481 cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg catggctccc
     9541 tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa cgcatcaacg
     9601 aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt ttactgcttt
     9661 ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct tcatgcacac
     9721 tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt agattttact
     9781 tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgctaa gcagggtgtt
     9841 atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta cacagcagaa
     9901 gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg ttttttatcg
     9961 ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg ctgttgttga
    10021 actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc tgtttggccg
    10081 ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg ccagctatta
    10141 catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca tgaccaccga
    10201 tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa tcgccgttag
    10261 tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc tccgacaact
    10321 gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg gtattatggt
    10381 ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg cgaccggcgt
    10441 tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa actggctttc
    10501 tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata attcattgct
    10561 taatccggcg aagaaattca cttatcaggc accgcaaaac gttgatgaca cttatgtcgt
    10621 gtttatcatc ggtgaaacca cgcgttggga ccatatgggc attttcggct atgagcgtaa
    10681 taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt actcctgtga
    10741 taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg cggaagataa
    10801 tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt taggattcag
    10861 ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga tggcggacaa
    10921 cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc cggtagatga
    10981 tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg gtaagcatct
    11041 gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc cgcgtagctt
    11101 cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag cgcagatgat
    11161 caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg tgattgatca
    11221 ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt caattaatga
    11281 acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt tccgcgtacc
    11341 gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgctc aggcatttgc
    11401 gcagctgaaa aaagaagccg acatgaaagt gccacgccgt catgtagagc tgtacgacac
    11461 catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa acaacaactg
    11521 gtgtcacatc ccgcagacaa aagtggcagc ggctaactaa gcggcctgct gactttctcg
    11581 ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg cagtaagatg
    11641 cgccccgcat tcggtgattg gcgcagcctg gtagcgcact tcgttcggga cgaaggggtc
    11701 ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcagcg agcaggcttt
    11761 tttgcatttg aagcccatga ggatgagaac ctccgggggc ggggaggttc gactcgagcg
    11821 aagcgagaga acgttgcgcc agcaacggcc cgcagggcga gccacgaagt ggcgagtaat
    11881 cctctatcac cgaccaaatt cgaaaagcct gctcagcgat caggcttttt ttgcatttgt
    11941 agcccatgag gatgagaacc tccgggggca gggaggttcg actcgacgta ggcctgataa
    12001 gacgcgccag cgtcgcatca ggcaacggca ccgcacttat tgtcggatgc ggcgcgaccg
    12061 ccttattcga ccaacacacc acacattttc ctgccaactt tgcgccccat atctcactat
    12121 ccattaacgg atttgtgaca gaaaccattg tcttttttta tatttgctta aatctttttt
    12181 tgcgttactt atggctggcg ctggcatttt ccgctgtgcg ttttagcgtt catggtatta
    12241 atcgctcaga tatccacctt ttcctcagat ttttttcatt aaatgcataa aacttaatca
    12301 ccaaatgttg ctaaatatta agctgattgt tctggtcatg ttggagtagc acaaatttct
    12361 ctgctgcaaa tagctgtttg aagttttttt aatctttgtt tgtggattct caccgttggt
    12421 gtaaatcccg gttggctgta ttgacgtttt cacattctgt tgacagattg taggtcacga
    12481 ggggcatttt atggaggatc cgcactgtta cactgatgtt aattagtacg gcatccccac
    12541 ctcataacgt tgacccgacc gggcaaaaaa caaaaaaggt caggcagcga caacccactg
    12601 caaagggtta aaacaacaaa catcacaatt ggagcagaat aatgcgtatt tccttgaaaa
    12661 agtcagggat gctgaagctt ggtctcagcc tggtggctat gaccgtcgca gcaagtgttc
    12721 aggctaaaac tctggtttat tgctcagaag gatctccgga agggtttaac ccgcagctgt
    12781 ttacctccgg caccacctat gacgcctctt ccgtaccgct ttataaccgc ctggttgaat
    12841 ttaaaatcgg caccactgaa gtgatcccgg gcctcgctga aaagtgggaa gtcagcgaag
    12901 acggtaaaac ctataccttc catctgcgta aaggtgtgaa gtggcatgac aataaagaat
    12961 tcaaaccgac gcgcgaactg aacgccgatg acgtggtgtt ctcgttcgat cgtcagaaaa
    13021 acgcgcaaaa cccgtaccat aaagtttctg gcggtagcta cgaatacttc gaaggcatgg
    13081 gcttgccgga gctgatcagc gaagtgaaaa aggtggacga caacacagtt cagttcgtgc
    13141 tgactcgccc ggaagcgccg ttcctcgctg atctggcaat ggacttcgcc tctattctgt
    13201 caaaagaata tgctgatgcg atgatgaaag ccggtacacc ggaaaaactg gacctcaacc
    13261 caatcggaac cggtccattc cagttacagc agtaccaaaa agattcccgt atccgctaca
    13321 aagcgtttga tggctactgg ggcaccaaac cgcagatcga tacgctggtc ttctctatta
    13381 cccctgatgc ttccgtgcgt tacgcgaaat tgcagaagaa tgaatgccag gtgatgccgt
    13441 acccgaaccc ggcagatatc gctcgcatga agcaggataa atccatcaac ctgatggaaa
    13501 tgccggggct gaacgtcggc tacctctcgt ataacgtgca gaaaaaaccg ctggatgacg
    13561 tgaaagttcg ccaggcactg acctacgcgg tgaacaaaga cgcgatcatc aaagcggttt
    13621 atcagggcgc gggtgtatca gcgaaaaacc tgatcccgcc aaccatgtgg ggctataacg
    13681 acgacgttca ggattacacc tacgatcctg aaaaagcgaa agccttgctg aaagaagcgg
    13741 gtctggaaaa aggtttctcc atcgacctgt gggcgatgcc ggtacaacgt ccgtataacc
    13801 cgaacgctcg ccgcatggcg gagatgattc aggcagactg ggcgaaagtg ggcgtgcagg
    13861 ccaaaatcgt cacctacgaa tggggtgagt acctcaagcg tgcgaaagat ggcgaacatc
    13921 agacggtaat gatgggctgg actggcgata acggggaccc ggataacttc ttcgccaccc
    13981 tgttcagctg cgccgcctct gaacaaggtt ctaactactc aaaatggtgc tacaaaccgt
    14041 ttgaagatct gattcaaccg gcgcgtgcta ccgacgacca taacaaacgc gttgaactgt
    14101 acaagcaagc gcaggtggtg atgcacgatc aggctccggc actgatcatc gctcactcca
    14161 ccgtgtttga accggtacgc aaagaagtca aaggctatgt ggttgatcca ttaggcaaac
    14221 atcacttcga aaacgtctct atcgaataat taaaagccat acaagactga tggcaaaggc
    14281 aaaactgcct gatgtgctcc gcttatcagg cctacgaaaa ttctgcaatg tattgaattt
    14341 gcacgatttt gtaggccgga taaggcgtta acgccgcatc cggcataaac aaagcgcact
    14401 ttgtcaacaa tctgtatacc cggtggcgct gtgcctctgc gtgcagcgcc atccggcagc
    14461 aatacttcat tctctgtccg acaagggcgg gaattgattt gtgagcaata cagacacgca
    14521 gttccaggct gcgggtcact atagagaatc cgggttatgt tgcagtttat tctccgacgt
    14581 ttgggactcg tcatccccac gtttatcggt attacccttc tcacatttgc ctttgtccac
    14641 atgatcccgg gcgatccggt gatgatcatg gcaggcgaac gtgggatctc ccctgagcgt
    14701 cacgcgcagc tgctggctga actcggctta gataaaccga tgtggcagca gtatctccat
    14761 tacatttggg gcgtaatgca cggcgatcta ggcatttcaa tgaaaagccg aattccagta
    14821 tgggaagagt tcgtgccgcg ctttcaggcc acgctggaac ttggcgtctg cgcgatgatt
    14881 tttgctacgg cagtcggtat tccggttggt gtgctggctg cggttaaacg cggttccatt
    14941 ttcgatcaca cagcggttgg cctggcgctg accggctact cgatgccgat cttctggtgg
    15001 ggcatgatgc tgatcatgct ggtttcggtg cactggaacc tgacgcccgt ctccggtcgc
    15061 gtgagcgata tggtgttcct cgatgactcc aatccgttaa ccggttttat gctaatcgac
    15121 accgccatct ggggtgaaga cggcaacttt atcgatgccg tcgcccatat gatcttgcct
    15181 gccattgtgc tgggtactat tccgctggcg gtcattgtgc gtatgacacg ctcctcgatg
    15241 ctggaagtgc tgggcgagga ttacatccgc accgcgcgcg ccaaagggct aacccgcatg
    15301 cgggtgatta tcgtccatgc gctgcgtaac gcgatgctgc cggtggtgac cgttatcggc
    15361 ctgcaggtgg gaacattgct ggcgggggcg attctgaccg aaaccatctt ctcgtggccc
    15421 ggtctgggac gctggttgat tgacgcactg caacgccgcg actatccggt agtgcagggc
    15481 ggcgtattgc tggtggcgac gatgattatc ctcgtcaact tgctggtcga tctgctgtac
    15541 ggcgtggtga acccgcgtat tcgtcataag aagtaagggg acatcatgtc acaggttact
    15601 gaaaataaag tgattagcgc accggtgccg atgaccccgt tacaggagtt ctggcactat
    15661 tttaaacgca acaaaggcgc ggtcgtcggg ctggtttacg tcgtcatcgt gctgttcatc
    15721 gcgatctttg ccaactggat tgcaccctat aacccggcgg aacagttccg cgatgcactg
    15781 ctcgccccgc cagcctggca ggaaggcggc agcatggcgc acttgctggg caccgatgac
    15841 gtaggccgtg atgtgctgtc gcgcctgatg tacggtgcgc gcctgtcgct gctggttggc
    15901 tgtctggtag ttgtgttatc gctgattatg ggcgttattc tcggcctgat cgccggttac
    15961 tttggcggcc tggtcgataa catcattatg cgcgtggtcg atatcatgct ggcgctgcca
    16021 agtctgctgc tggcgctggt gctggtggca attttcggcc cgtcgattgg taacgccgcg
    16081 ctggcactga ccttcgttgc cttgccgcac tatgtgcgct taacccgcgc cgccgtgctg
    16141 gtggaagtta accgcgatta cgtcaccgcg tctcgcgtgg cgggtgccgg ggcgatgcgt
    16201 cagatgttta ttaacatctt cccgaactgc cttgcgccgc tgattgttca ggcgtcgctc
    16261 ggtttctcta acgccattct cgatatggct gctcttggtt tcctcggcat gggggcacag
    16321 ccgccaacgc ctgagtgggg caccatgctc tccgacgtgt tgcagttcgc gcaaagcgcc
    16381 tggtgggtcg tgaccttccc gggtctggcg atcctgctga cggtgctggc atttaacctg
    16441 atgggtgacg gtctgcgtga cgcgctcgat cccaaactga agcagtaaga ggttcgagat
    16501 ggcgttatta aatgtagata aattatcggt gcatttcggc gacgaaagcg cgccgttccg
    16561 cgccgtagac cgcatcagct acagcgtaaa acagggcgaa gtggtcggga ttgtgggtga
    16621 gtccggctcc ggtaagtcgg tcagttcact ggcgattatg gggctgattg attatccggg
    16681 ccgcgtaatg gcagaaaaac tggagtttaa cggccaggat ttgcagcgta tctcagaaaa
    16741 agagcgccgc aacctggtgg gtgccgaagt ggcgatgatc ttccaggacc cgatgaccag
    16801 ccttaacccg tgctacaccg tgggtttcca gatcatggaa gcgattaagg tgcatcaggg
    16861 cggtaataag agcactcgcc gtcagcgggc gattgacctg ctgaatcagg tcggtattcc
    16921 cgatccggca tcgcgtctgg atgtttaccc gcatcagctt tccggcggca tgagccagcg
    16981 cgtgatgatc gccatggcga ttgcctgtcg gccaaaactg ctgattgccg atgaaccgac
    17041 caccgcgctg gacgtgacca ttcaggcgca aatcatcgaa ctactgctgg agctacagca
    17101 gaaagagaac atggcgctgg tgttaattac ccatgacctg gcgctggtgg cggaagcggc
    17161 acataaaatc atcgtgatgt atgcaggcca ggtggtggaa accggtgatg cgcacgccat
    17221 cttccatgcg ccgcgtcacc cgtatactca ggcattgctg cgtgcgctgc cagaatttgc
    17281 tcaggacaaa gaacgtctgg cgtcgttgcc aggtgtcgtt cccggtaagt acgaccgccc
    17341 gaacggctgc ctgcttaacc cgcgctgccc ctatgccact gacagatgtc gcgctgaaga
    17401 accggcgctg aatatgctcg ctgatgggcg tcagtccaaa tgccattacc cacttgatga
    17461 tgccgggagg ccgacactat gagtacgcaa gaggccacct cgcaacaacc gctgttgcag
    17521 gctatcgacc tgaaaaaaca ttatccggtg aagaaaggca tgttcgcgcc ggaacgtctg
    17581 gtgaaggcgc tggacggcgt ttcgtttaac cttgaacgtg gcaaaacgct ggcagtagtg
    17641 ggcgaatctg gctgcggtaa atcgaccctc ggtcggttgc tgacgatgat cgaaacgccc
    17701 accggtggtg agctgtatta ccaggggcag gatctgctca agcacgatcc gcaggcgcag
    17761 aagctgcgtc ggcagaaaat ccagattgtc ttccagaacc cttacggttc gctgaatccg
    17821 cgtaaaaaag ttgggcaaat tctcgaagag ccgctgctta ttaacaccag cttcagcaaa
    17881 gaacagcgtc gggaaaaagc cctgtcgatg atggcgaaag tcggcctgaa aaccgagcac
    17941 tatgaccgct atccgcatat gttctccggc ggacaacgtc agcgtatcgc tattgcccgt
    18001 ggtctgatgc tcgacccgga cgtggtgatt gccgatgaac cggtttctgc gctggacgtc
    18061 tcagtgcgtg cgcaggtgct gaatctgatg atggatttgc agcaggagtt ggggctttct
    18121 tatgtcttta tctcccacga cctgtcggtt gtggagcaca ttgctgatga agtgatggtg
    18181 atgtacctgg gccgctgcgt ggagaaagga acgaaggacc aaatcttcaa taacccgcgc
    18241 catccgtaca cccaggcgct gctctccgcg acgccacgcc tgaacccgga cgatcgtcgt
    18301 gaacgcatca aactcaccgg tgaactgcca agcccgctga atcctccgcc gggttgcgcc
    18361 ttcaacgccc gctgtcgtcg gcgcttcggc ccctgcaccc agttgcagcc gcagctaaaa
    18421 gactacggcg gtcaactggt agcttgtttt gctgttgatc aggatgaaaa tccgcagcgt
    18481 taactcacaa aaccggggcc atgcgctccg gtttactcaa tcagcttcaa cagtggcgac
    18541 aagcatagca aaatgccgta aagcagaatt agccaggctt taaatccgcg aagtttttcc
    18601 agttgtgcga ctttatagat caggaaaaac ggaatgaggc acgacacaat tccatataac
    18661 gggcttccca actgaaagaa caccagcacc gatacacgaa acgaaaccca aatcgtcaat
    18721 gtaatgacga taaaagcgca gatcgccaga gtcagcatgc gtgagttaat tttcttggta
    18781 tcaataattc ggcttaacag attgagaata atgcctttaa tagcctcgtg gaaaccgaga
    18841 taaatgccaa agaatgcggt cagtacggca aagatattca gcaccgtaga ggtgatatga
    18901 atgatatgtc cagggatcac ctgcgcggcc agcgccagtg ccgagatatt ttgttcaaag
    18961 gcagaaacgg cttcttcgtg gctaattgag aaggtaaacg aaaaggcaaa aaacaggatc
    19021 accgcgatga gcgtgatata actaattcgg tgggtacgca gcgcgagccg ggttgccagt
    19081 actttatctg cttcccgttt acgataggca atattcattg ggttaagtac ctgaataaat
    19141 accgcagaaa agaagcaaaa tggaattgta agcaggacat cgcggaaaaa gacggaggct
    19201 tgcgggaagg cggttatatt ggcgaaattc cagtgcggga tcatcgcaaa accgaacacg
    19261 acaataatcc ctactttgac caccaccatt ggcccggaaa tcttaaacag taatcgttca
    19321 ccaccagacg caatcgccac cagtacggcg aaaatagcga ctttataaag tagagattgt
    19381 gaaagatcgg catcggttaa accgaaggtt ttcaggtacg aggcgctgtc gaaaaccacg
    19441 gagagagagt agataaaaat cccgtggata atcatcaaaa agtagataac cccgaggaaa
    19501 attccccagt tcttccccag gtaatgactg ataatatcgg tgtagtcatt acaggaatcg
    19561 ctttcagaaa gggtttttaa ataaatgtcc tgcactaccc aggtggcagg ataagcaatg
    19621 atcgctgcgg taataaatac ccaaattccc ttcaagccaa tttgtactgg catcagcacg
    19681 gttccggcac caatcgccat gcctatgcat aataaaaccc agccgaagtc gtagcgtgta
    19741 aacggcaatt tctgattgtg tttcgatagt gtgttgtgct gcataaagtc ctgtccagaa
    19801 agaagtcggc ctcgcaaaaa tgaataaacc cagtttctct tgcgcaacag gtggagaagt
    19861 cgaatacgca gtatgtaaaa aatgaggcaa cagattcact ggggaggtcg cgaggaggcg
    19921 caattctaca aaattgcgca tttcactacc agtctggagt cagcccggat atgaactgca
    19981 actattgcaa ataaagaaat taagctggaa ggcatcgcgc aatgaggaag attgccgggt
    20041 gctgacagta aaaagcaatc gtaaaatcag cggcaagccg gaagggttcc ggtgaggcgc
    20101 aatgttgcgg gggctttatc cctggtggca ttggttgctg gagagagaaa acccccgcac
    20161 gttgcaggta tatacctgac aacataacgg gggctaatct tgaacctaga c
//
LOCUS       DAFNBJ010000085        20135 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000085 DAFNBJ010000000
VERSION     DAFNBJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.085"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(305..871)
                     /locus_tag="L2X49_002922"
                     /pseudo
     CDS             complement(305..871)
                     /locus_tag="L2X49_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(893..2165)
                     /gene="istA"
                     /locus_tag="L2X49_002923"
                     /pseudo
     CDS             complement(893..2165)
                     /gene="istA"
                     /locus_tag="L2X49_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215941.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(2249..2518)
                     /locus_tag="L2X49_002924"
                     /pseudo
     CDS             complement(2249..2518)
                     /locus_tag="L2X49_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(2529..2786)
                     /locus_tag="L2X49_002925"
     CDS             complement(2529..2786)
                     /locus_tag="L2X49_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhX family toxin"
                     /protein_id="HBN4797981.1"
                     /translation="MNLSRQGQRTLHVLAKSGRIVHVRDASGRVTSVECYSREGLLLA
                     DCTLTVFKKLKTQKLIKSVNGQPYRINTTGLNNVRSQPDNR"
     gene            complement(3084..3152)
                     /locus_tag="L2X49_002926"
                     /pseudo
     CDS             complement(3084..3152)
                     /locus_tag="L2X49_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227376.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(3161..3904)
                     /locus_tag="L2X49_002927"
     CDS             complement(3161..3904)
                     /locus_tag="L2X49_002927"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HBN4797982.1"
                     /translation="MNRPFSGLNRAECVSLMKRYPLSIGILAGQWLKLGEYLQTLRAL
                     EQPVLHLDLMDGHFCPQFTVGPWAITQLPAQIIKDVHLMVDNPWPVVQASVKAGAHCV
                     TLQVENSPHLHHMLHWLGEQNTEVSGATMPVLRGISLCPATPLEHILPVIEDVEIVQI
                     LAVNPGYGSKLSKPALLRRIAQVVALLNASGKRDSILLAVDGSLTLEELPEIIASGVD
                     RVVSGSALFRNDALAENISRWLTIIQSAK"
     gene            complement(3915..4205)
                     /gene="lsrG"
                     /locus_tag="L2X49_002928"
     CDS             complement(3915..4205)
                     /gene="lsrG"
                     /locus_tag="L2X49_002928"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBN4797983.1"
                     /translation="MHVTLVEINVHDDKVDEFIDVFRLNHLGSVREEGNLRFDVLQDP
                     EVKSRFYIYEAYKDEAAVAFHKTTPHYLACVEKLESLMSCPRKKRIFNGLMP"
     gene            complement(4254..5129)
                     /gene="lsrF"
                     /locus_tag="L2X49_002929"
     CDS             complement(4254..5129)
                     /gene="lsrF"
                     /locus_tag="L2X49_002929"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBN4797984.1"
                     /translation="MADLDDIKDGKDFHTDAPQRNTPFTLKGCGALDWGMQSRLSRIF
                     NPETGRTVMLAFDHGYFQGPTTGLERIDINIAPLFPLADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILTELSNEAVALSIDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLI
                     DAGIKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIAAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSEHPVAMMKAVHAVVHRN
                     ETADRAYELYLSEKN"
     gene            complement(5158..6180)
                     /gene="lsrB"
                     /locus_tag="L2X49_002930"
     CDS             complement(5158..6180)
                     /gene="lsrB"
                     /locus_tag="L2X49_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HBN4797985.1"
                     /translation="MTIQSIKKIAVISTLSLAAFTLNAYAAERIAFIPKLVGVGFFTS
                     GGNGALEAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIVVSAVSPDGLCPAL
                     KRAMQRGVKVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAANQVKKEKAKVAFFYS
                     SPTVTDQNQWVKEAKDKIAKDHPGWEVVTTQFGYNDATKSLQTAEGIIKAYADLDAII
                     APDANALPAAAQAAENLKNTSVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGDMNTGDKLDIPGVGQVEVSPNSVQGYSYEAKGNGIVLLPERVVFTKEN
                     IDKYDF"
     gene            complement(6207..7205)
                     /gene="lsrD"
                     /locus_tag="L2X49_002931"
     CDS             complement(6207..7205)
                     /gene="lsrD"
                     /locus_tag="L2X49_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBN4797986.1"
                     /translation="MTRLHRYGWELALAALLMIEILGFGALNPRLLDLNVLLFSTSDF
                     ICIGIVALPLTMVIVSGGIDISFGSTMGLCAIALGVMFQGGVPLPLAIILTLALGALC
                     GLINAGLIIYTGVNPLIITLGTLYLFGGSALLLSGMAGATGYEGIGGFPQGFTDFANL
                     DVLDLPVPLIFFLVSVIFFWLLMHRTHAGRNVFLIGQSPRVALYSAIPVNRTLYALYA
                     MTGFASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSILGTAIAAL
                     LVGYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKDWLARRRAIRQA"
     gene            complement(7205..8251)
                     /gene="lsrC"
                     /locus_tag="L2X49_002932"
     CDS             complement(7205..8251)
                     /gene="lsrC"
                     /locus_tag="L2X49_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBN4797987.1"
                     /translation="MLKFIQNNREATALLAVLLLFVLPGMLDSQYLSVQTLTMIFSSS
                     QILMLLAMGSTLVMLTRNIDVSVGSITGMCAVLLGLLLNAGYPLPVACVATLLLGLLA
                     GFINGVLVAWLKIPAIVATLGTLGLYRGVMLLWTGGKWIEGLPAQLKQLSAPVFLGIS
                     AIGWLTLLLMLLMAWLLAKTAFGRCFYATGDNLQGARQLGVRTEAIRILAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKVIAACVLGGISLLGGSGTIIGAVLGAYFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNIRRQKYARFTPTPQPRPSAK
                     NADSVRTAKKREVA"
     gene            complement(8245..9798)
                     /gene="lsrA"
                     /locus_tag="L2X49_002933"
     CDS             complement(8245..9798)
                     /gene="lsrA"
                     /locus_tag="L2X49_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBN4797988.1"
                     /translation="MQMSQEKIAPLLSAQSVCKQYSGVNVLKGIDFFLCKGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGIIEIEGARCTKLTPILAHQLGIYLVPQEPLLFPSLT
                     VKENILFGLPKHQSSAQKMAAMLTALGCQFDINSPAGSLDVADRQMVEIMRGLMRDSR
                     ILILDEPTASLTPAETDRLFSRLRELLSTGVGIVFISHKLPEIRQIADYISVMRDGII
                     ALSGKTADLTTDEIIQAITPASRTNALSATQKLWLELPGNRPQHEAGTPVLSLENVTG
                     EGFMNVSFEVRAGEILGLAGLVGAGRTELAETLYGLRPLQAGSIVLGGKDISSLSTRE
                     RLQHGLVYLPEDRQSSGLNLDASLAWNVCALTHNHSGFWANPAKDSATLERYRRALNI
                     KLNDPEQPARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDLEEIEQMADRVYVMHQGETDNDALQGDEINVDTIMHVAFGDRTSGA
                     TPHSGEAPC"
     gene            10048..11004
                     /gene="lsrR"
                     /locus_tag="L2X49_002934"
     CDS             10048..11004
                     /gene="lsrR"
                     /locus_tag="L2X49_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBN4797989.1"
                     /translation="MTINDPIISEQGMCEEELVARIAWFYYHDGMTQSDISNRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETELRRRFDLNNVRVIPGLPGADVCIRL
                     GIGAAHMLMESLKPQQILAAGFGEATMNTLKRLSGFISAQQIRLVTLSGGVGPYMTGI
                     GQLNAACPVSIIPAPLRASSAEIAQTLKNENSVHDVLLAAQAADAAIVGIGAINQKDE
                     ATIIRSGYITKGEQLMIGRKGAVGDILGYFFDGEGEVIPEMKIHDELIGLTLSTLKTI
                     PTVIGVAGGVNKAEAIAAAMKGGYINALVTDQETAAAIVSGQ"
     gene            11104..12696
                     /gene="lsrK"
                     /locus_tag="L2X49_002935"
     CDS             11104..12696
                     /gene="lsrK"
                     /locus_tag="L2X49_002935"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HBN4797990.1"
                     /translation="MAQLCTLSDSEHYLMALDAGTGSVRAVIFDLEGKQIAVGQAEWQ
                     HFAVPDVPGSMEFDLGKNWQLTCECIRQALHNAGIAPGAIAAVSACSMREGIVVYDAE
                     GTPVWACANVDARAAREVSELKEIHDYTFESEVYRSTGQTLALSAIPRLLWLAHHRSG
                     IYRRASTVTMISDWMAYMLSGELAVDPSNAGTTGLLDLKTRDWKPSLLQMAGLRSDIL
                     SPVKETGTLLGRVTAQAAALCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLPTPIVDPEMNVRVNPHVISGMVQTESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGVDAYTLLEEMASRVPAGAWGVMPIFSDRMRFKSWYHAAPSFINLSIDPEKCNKAT
                     LFRALEENAAIVSTCNLQQIADFTGIHPTSLVFAGGGSKGKLWSQILADVSGLTVNVP
                     VVKEATALGCAIAAGVGAGIWSSMDETGERLVRWEREHVPDREKHALYRESREKWQAV
                     YQEQLGLVDHGLTTSLWKAPGL"
     gene            12709..13059
                     /locus_tag="L2X49_002936"
     CDS             12709..13059
                     /locus_tag="L2X49_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBN4797991.1"
                     /translation="MIKSNEAQHDYRFGDSGPKYLIRGPMCDMGVVMLQPGQSFPNHR
                     HIEACEIFYTLAGEVTLYLNGEPYTLGSGDVMHCDPGEAHFLVNKGEVPWKGVFVKSP
                     HIPGDSHPVEPTMY"
     gene            13067..13147
                     /locus_tag="L2X49_002937"
                     /pseudo
     CDS             13067..13147
                     /locus_tag="L2X49_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(13260..13340)
                     /locus_tag="L2X49_002938"
                     /pseudo
     CDS             complement(13260..13340)
                     /locus_tag="L2X49_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13337..13586)
                     /locus_tag="L2X49_002939"
                     /pseudo
     CDS             complement(13337..13586)
                     /locus_tag="L2X49_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13662..14135)
                     /locus_tag="L2X49_002940"
                     /pseudo
     CDS             complement(13662..14135)
                     /locus_tag="L2X49_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(14225..15733)
                     /gene="ltrA"
                     /locus_tag="L2X49_002941"
     CDS             complement(14225..15733)
                     /gene="ltrA"
                     /locus_tag="L2X49_002941"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBN4797992.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            complement(16406..17145)
                     /locus_tag="L2X49_002942"
                     /pseudo
     CDS             complement(16406..17145)
                     /locus_tag="L2X49_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(16748..16864)
                     /locus_tag="L2X49_002942"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(17866..18291)
                     /locus_tag="L2X49_002943"
     CDS             complement(17866..18291)
                     /locus_tag="L2X49_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797993.1"
                     /translation="MNGIKTGLGITPGEHIISAASALSRNIRHCFCLSCRGRLILQTD
                     AQGAWFEHDLHALSAQQKAACVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGQHF
                     PGKKFCEACGTGIYSRATCTKSVYSYQPDLFQDCGGAST"
     gene            complement(18288..18671)
                     /locus_tag="L2X49_002944"
     CDS             complement(18288..18671)
                     /locus_tag="L2X49_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4797994.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDGLTEHGQQCPYVRPERREIQLIKRLQKFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            complement(19043..19642)
                     /locus_tag="L2X49_002945"
     CDS             complement(19043..19642)
                     /locus_tag="L2X49_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBN4797995.1"
                     /translation="MNTYNANPGYEMDPQLLTHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNALRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
     gene            complement(19874..20023)
                     /locus_tag="L2X49_002946"
     CDS             complement(19874..20023)
                     /locus_tag="L2X49_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HBN4797996.1"
                     /translation="MEKVISHARYKLTPEEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVT"
BASE COUNT         4690 a         5415 c         5328 g         4702 t
ORIGIN      
        1 tgaaacttct agctatatta ataaatgtaa tgtgtgaatg atgatggtgc ttgctttaac
       61 tatagattgt tattccatat agaaattatg tagcatcgca attaagtttt attgataggc
      121 atgagtccgg ttcaataccg acttaaacat ttttcgtagg atatctaagg ctgcggcgca
      181 gatcacttat taggtggaca ccactttgtc gcatgcttta tattatgacc agatggctct
      241 cgctgtacgc ttacttttct ttcactccac agcaatattc tgccacaaac accgccagta
      301 gaagtcatat tttcttattt atcctgtaaa gcagaccact gcacagtgct ccagcaaaaa
      361 gtacagctgc aatggtggca aacatcagtg tatatgcaaa ttctttatac gtatcaagaa
      421 ggtatccgaa gataaggtgg acataagctt ccgggctgaa tccgatgcaa attaatatcc
      481 ccattgcggt tccggtaaac tcttttggta tacgaatttc atttacctgt gcatacatta
      541 taccgcgcat accaaacgta agggctccca gcaataaaac cagagtaata agcagtataa
      601 gaaagtgtga gtcatgaggg ataaatataa aagatgcagt tatcaatgcg cctagagtaa
      661 agaaactggc cataactttc agggaagaac caatcttatc tgcaaccagg ccagcaaaag
      721 gagaaataat aaaagcgaga aaatatgctc taatcatccc gataacagca gtttgatcgg
      781 gagtcattcc ataggcatcg gagatgtatg gtaccagata agcgccgcct tggtaaacaa
      841 aaataacaca gctgataata aatgctgcta acgcagccat caccagggga gggccggtgg
      901 cgaatgtgaa gtcatactct tcgaacgttt tcaccgccgg gaaggctgcc attcgggtat
      961 acatcgcctg tttacgttga tgacgtgcca gtttttcttc atgaagcaga tgctccagga
     1021 agtccatata actccattcc tggtctactg cctgttgtga cagcgcaggc gctgcgctta
     1081 taaggctttc cagttgcaac tgcccggcga gcgccatcag tcgttgatgt tgcagttcca
     1141 tcatcacgcc actcttctgc agaatgagtc gtagatggag agtggatgat gcaggggggg
     1201 ttgtcgaagt tcaccagatt ttcaccagaa tgcacgtcat actctttttt ctccggaggc
     1261 agtgccagca tggactgctg ctcttcgagc cagcgatcgc agggacgggg ctggattgtt
     1321 tcatgctttc gttggttagc gacatcgtgc agccagcgca gaccgtggcg gttggctgtt
     1381 tcaacatcga cagtgatccc catcgggcgc aggcgggtca ttagtgggat gtaaaaactg
     1441 ttacgggtgt actgcaccat ccgttccacc ttacctttag tctgtgccct gaaggggcga
     1501 cacagtcggg gagagaagcc catctccttg ccgaactgcc acagcgaagg atggaaccgg
     1561 tgctgaccgg tctgatatgc gtcacgttgc agaaccacag ttttcatatt gtcatacaac
     1621 acttagcgcg gcacaccacc aaagaagcgg aacgcattac gatggcaggt ctccagcgtg
     1681 tcataacgca tattgtcagt gaattcgatg tacagtattc cgctgtatcc gagaacaaca
     1741 acgaacacgt gaagcggtga gcgatcatta cgcatagtgc cccagtcaac ctgcatctgt
     1801 cgtccgggtt cagtttcgaa ccgaacggca ggctcctgct cctgaggaac cgagagagaa
     1861 cgaatgaatg ccctgagaat ggtcattccg ccacgatatc cctggtctct gatctcgcga
     1921 gcgattaccg ttgccgtgat tttgtaagga tgagcatcgg cgatgcgttg acgaatataa
     1981 tcccggtatt catccaggag tgaagcaaca gcaggtcgcg gcgtatattt tggcggctca
     2041 gattttgcct gcaaataacg tttaacggta ttgcgggaga gccccagttc tctggcaatc
     2101 gcccggctac tcattccctg cttgtgcagg attttaattt ccataactgt ctcaaaagtg
     2161 accacaagct ctcctgaatc aggagagcag attcccccct ggatctgatt tcaggcgttg
     2221 ggtgtggatc actattgcac cgttcgtgac agcaatatcc acgctccgcc agcaagtggg
     2281 tgtataaacc cgggccgcag ccgatatcca gagtgcgtgc accaaccagt agctgacggg
     2341 caatccaccc aacctgctgt tcaattacga tctgtctgcg gctggcccag tcatcatcct
     2401 gcgacagatg gttttccagc atgcgctggc taaacgccag ctcttcccag ggtattttac
     2461 tttcagctgc agacagctgc agccgaccgg aggaagaaat gagagtactg atatccataa
     2521 tgctctcctt aacggttatc gggctgtgag cgaacgttgt tcagcccggt ggtgttgatg
     2581 cggtaaggct gaccgttgac ggacttgatc agtttttggg ttttgagctt tttgaaaacg
     2641 gtgagagtac agtcggcgag caacagccct tcgcggctgt agcattcaac agaggtaacg
     2701 cgaccagatg catcgcggac gtgcacaata cgtccacttt ttgcgagcac atgtaaggtg
     2761 cgctgtccct gacgggataa attcacactg gaaaacctgt taatcaacat gagcaaaaac
     2821 gagccaacat ccgcagatgt tcgcgtaaag tttaagcgca gcgataaaca gtccattctg
     2881 agcagaacgg aaaactgatt atctgatgat tacattctcc agcatcaaag cctcggtgag
     2941 agttgaaagg tatttacggg atgaataata accactgttt tttataatgg aaaaaccgga
     3001 gagaaaaaat gtgatgcggg aggaaatttc aaaaggtaat actggcaaca tttgttgcca
     3061 gtcagtgaac ttgtcaacca ttccatcaat gggttgtaga atctgatggc gccaggcaaa
     3121 tcattggtcc caagcataat ataagaaaac atacccttcc tcatttcgct gattgaatta
     3181 tagtaagcca gcgggagata ttttccgcca gagcatcgtt cctgaagaga gcgctgccgg
     3241 atacaacacg atcaacgccg ctggcgatta tctccggcag ttcttccagt gttagcgaac
     3301 cgtcaacggc cagcagaatg gaatcccgct tcccggacgc attgagtaat gcgacaacct
     3361 gagcgatgcg cctcagtaag gcgggcttgc tgagtttgct accgtaaccc ggattgaccg
     3421 ccagaatttg tacgatttca acgtcttcaa tgaccggcag aatgtgttct agcggtgtcg
     3481 ctgggcacag gctaatccct cgcaacaccg gcattgtggc gccggaaact tccgtatttt
     3541 gctcacctag ccagtggagc atatggtgca gatgcggcga gttttctacc tgcaacgtaa
     3601 cgcagtgcgc gcccgccttt acgcttgcct gcacgacggg ccagggatta tccaccatca
     3661 ggtgaacatc cttaattatc tgcgctggta attgcgtgat agcccagggt ccgacggtga
     3721 actgagggca aaaatgcccg tccatcagat ccaggtgaag aacgggttgt tcaagcgcgc
     3781 gaagcgtttg caggtactca ccgagtttga gccactggcc ggcaagaata ccgatgctta
     3841 acgggtaacg cttcattaac gagacgcact ctgcgcgatt caacccggaa aagggacggt
     3901 tcattttgag atccttatgg catcaatcca ttgaagatgc gtttttttcg ggggcagctc
     3961 atcagcgatt caagtttttc cacgcaagcg aggtagtgag gcgttgtttt atggaacgca
     4021 acggcggctt catctttata ggcttcataa atataaaagc ggctcttcac ttccggatcc
     4081 tgcaatacgt cgaagcggag attcccctct tcacgcaccg atcccagatg attgaggcga
     4141 aagacgtcga taaattcatc gactttatcg tcgtggacgt taatttctac cagagtgaca
     4201 tgcatacagt tctccttgaa acgcatgggc ccgcctggca ggcccgtgac ttcttagttt
     4261 ttctcgctca gatagagttc atacgcgcgg tctgcggttt cattacgatg taccaccgcg
     4321 tggacggcct tcatcatggc gacagggtgt tcggactgga agatgttacg tcccatatcg
     4381 acaccggatg cgccctggtc gatggcctgc cagcacatct caagcgcttc gcgttccggt
     4441 aattttttac caccggcgat aacaatcgga accgggcagc ctgcggcgat gcgctcaaaa
     4501 cctttatcga catagtaggt cttgatgatc tgcgccccca tctctgcggc gatacgcgtg
     4561 gcgagtgaga agtagcgctg atcgcgcacc atgtctttcc ccacgccggt tactgccatc
     4621 gtcggcatgc cgactttgat accggcatcg atcaactgaa tgatattttt gatcgactgg
     4681 tgctcatact cactgccgat atacacctgc gccgccacgg cgcaactgtt gagacgaacg
     4741 gcatcatcaa tggaaagtgc gaccgcttca ttgcttaact cggtgagaat agagttggcc
     4801 cccgaggcgc gcagtaccac cggcttgtta gtggcaggcg gcaccacgct gcgcagaata
     4861 ccgcgtgtac acatcagcac atcggcgagc gggaacaggg gggcgatatt aatatcgata
     4921 cgctccagtc ccgttgtcgg cccctgaaaa tagccgtggt caaacgcgag catgaccgtt
     4981 cttccagtct caggattaaa aatgcgcgac agtcgggact gcatccccca gtcaagcgcg
     5041 ccgcatccct taagcgtgaa cggggtgttg cgctgcggcg catcggtatg gaaatccttg
     5101 ccatctttga tgtcatctaa atcagccatt ttttactcca tggttaaggg atgaaactca
     5161 gaaatcgtat ttgtcgatgt tctctttagt gaacaccacg cgttcaggca gcagcacaat
     5221 gccattgccc ttcgcctcgt agctgtagcc ctgtacgctg ttcggcgaga cctcgacctg
     5281 gcccacgccc ggaatatcca gcttgtcgcc ggtattcatg tcgccttttt tcagcaacgc
     5341 atcagccacg tacacggaga tcttgccctg ctggaccacg tcccagaggc caaactcttt
     5401 caccgtgccg cgttcaacat aaggacgcat cacgtttggc gtgctgaatc cgacaattgc
     5461 cacgctggtg tttttcaggt tttcagcggc ctgggcagca gcgggaagag cgttggcatc
     5521 cggtgcgatg atggcgtcaa gatcggcata cgctttaatg atgccttctg cggtttgcag
     5581 cgatttggtg gcgtcgttat aaccaaactg cgtggtgacg acttcccagc ccggatgatc
     5641 tttggcgatc ttgtctttcg cttctttcac ccactggttc tgatcggtca cggtcggact
     5701 ggagtagaag aaggccactt tggctttctc ttttttgacc tgatttgcgg ccatatcgac
     5761 cagcatgccg cctaactggg caggagtgcc ctggttgatg tagtacgacc ggcattccgg
     5821 cttggtgtcg gaatcccagg tcagcacctt cacgccgcgc tgcatggcac gctttaacgc
     5881 cgggcataaa ccatcaggcg acacggcgga aaccacaatg gcgttatacc cttggttgac
     5941 gaagttattg atgagctgaa cctggccgga cacgctgggc tccgtcgggc catcgtaggt
     6001 gacatcgacg cccagctctt tgcctgcctc tagggcgccg ttaccgccgc tggtaaagaa
     6061 tcccacgccg accagtttcg ggataaatgc gattcgctct gcggcataag cgttgagagt
     6121 gaacgccgcc aggctaaggg tactgattac ggcaattttt ttaatgctct gaatagtcat
     6181 gtacttttcc tttatggcaa tgggtttcag gcctgccgga tagcgcgacg acgcgccagc
     6241 cagtctttga tttgctgacg atgcaggctg acagaacgac ctacgacaac gacaattagc
     6301 aatgcgccgg agagggcgct ggatacctga ttgggcacgc cagccatctg caatccctgc
     6361 tgcagatagc ccaccaacag cgcggcgatg gcagtaccga gaattgaccc ggagccgccg
     6421 tagatattgg cgccgcccag gacgaccgct gtaatagctg gcatcaggaa agaggcaccc
     6481 agatcggaac gtgcagagcc aaagtaagag accagcagca ccgccgcgac cgctgacgca
     6541 aagccggtca ttgcatacag cgcgtacaac gtccggttca ccgggatagc gctatacagc
     6601 gccacgcggg ggctctgccc aatcaggaag acattgcgtc cggcgtgggt ccggtgcatc
     6661 agcaaccaga aaaagatcac gctgacgaga aagaagatca gggggacggg cagatccagc
     6721 acatcgagat tggcaaaatc ggtgaagccc tgcgggaagc cgccgatacc ttcataaccg
     6781 gtcgctcccg ccatccccga caacagcagt gcgctgccgc cgaacaggta taacgttccc
     6841 agggtgatga tcagcgggtt cacaccggtg tagataatca atccggcgtt gatcagtccg
     6901 cacagcgcgc ccagcgcgag cgtcagaata atggccagcg gcagcggtac gccgccctgg
     6961 aacatcacgc ccagcgcgat ggcgcacaac cccatcgtcg agccaaagga gatgtcgatc
     7021 ccgccgctga cgatcaccat ggttaaaggc agggcgacaa tgccaatgca gataaagtcg
     7081 ctggtgctga acagcaggac gttcagatcc aacaggcgtg ggttcagggc gccgaatccc
     7141 agaatttcaa tcatcagtag cgcggcgagc gccagttccc agccgtaacg atgtaaacga
     7201 gtcatcaggc aacctcacgt tttttggcag tacgcacaga gtcggcgttt tttgccgacg
     7261 gacgcggttg gggcgtcggc gtgaagcggg catatttttg ccgacggata ttgcgttcca
     7321 gcgcgcagcg caaccggccg tcaaatacca gcaccgccag caggaccaga ccggcaataa
     7381 agtcattcca ccacgctgga atgcgcagca ggacgagcac gctgtcgatt tgcgtcagga
     7441 aataggcacc gagcacggca ccaatgatcg tcccggaacc gcccagcagg ctgatgcctc
     7501 ccagcacgca ggcggcaatg actttcattt ccagcccggt tcccgtctga ttcgggataa
     7561 agccaatctg tgaggcaaag acgatcccgg ccagcgctgc catgcagcca ttgagcgaaa
     7621 aggccagaat ccgaatggct tcggtacgca cgcccagttg ccgcgcgcct tgtaaattat
     7681 ccccagtggc ataaaaacag cgaccaaaag cggtttttgc cagcagccag gccatcagca
     7741 acatcagcag cagggtcagc cagccaatgg cggagatacc gaggaaaacc ggggcggaga
     7801 gttgcttaag ttgtgccggt aatccttcaa tccatttacc gcccgtccac agcagcatga
     7861 cgccgcgata taagccaagc gtgcccagcg tggcgacaat cgccgggatc ttcagccagg
     7921 caaccagcac gccgttgata aaccccgcca gcagtcccag cagcagcgtc gcgacgcagg
     7981 ccacaggcaa gggatatcca gcgtttaaca gcagtcccaa cagcacggcg cacatcccgg
     8041 tgatcgatcc gaccgacacg tcgatgttgc gggtcagcat caccagcgtg gagcccatcg
     8101 ccagcagcat gaggatttgg gagctgctga aaatcattgt cagcgtctga acgctgaggt
     8161 actggctgtc cagcatccct ggcagcacga acagcagcag cacggctaac agggcggtgg
     8221 cctcgcggtt attctggata aatttcagca tggcgcctcc ccggaatgtg gtgtagcccc
     8281 tgacgtgcgg tcgccaaacg ccacgtgcat gatggtgtcg acattaatct cgtcgccctg
     8341 cagagcatcg ttgtccgttt ctccctggtg catgacgtaa acgcgatcgg ccatctgttc
     8401 tatctcctcc agatcagaag agatgaacag aaccgcgaca ttctgggcgg caatactgcg
     8461 gagcaactga tagatgtcgt tacgcgccga gacgtccaca ccacgcgttg gctcatccac
     8521 aatcagcacc tgcggcgagg cttcaaggca tttggcgatc aggatttttt gctgattacc
     8581 gccagaaaga gtgcgggcag gctgttctgg atcgttgagc ttgatattaa gcgcgcggcg
     8641 atagcgttcg agcgtggcgc tgtctttggc cggattagcc cagaaaccgc tgtgattgtg
     8701 ggttaatgcg caaacattcc acgccagcga ggcatccaga ttcagtcctg acgattgccg
     8761 gtcctccggc aaatacacca gaccgtgctg tagacgttcg cgggtggaaa gtgaggagat
     8821 atccttgccg ccgagtacga tgcttccggc ctgtagcggt cgtagcccgt aaagcgtttc
     8881 ggcaagttcg gtacgtccgg caccgaccag accggccagg cctaaaattt ccccggcgcg
     8941 gacctcgaaa ctgacgttca taaacccttc gccagtcaca ttctcaagac tcaacaccgg
     9001 cgtgcccgcc tcgtgctgcg gacggttgcc gggcaattcc agccacagtt tttgcgtcgc
     9061 gctcagcgcg ttggtgcgtg aggcaggcgt gatggcctgg ataatctcgt ccgtggtcag
     9121 gtccgctgtt tttccggata gcgcaatgat gccgtcccgc atcacgctaa tgtaatccgc
     9181 tatctggcgt atctctggca gcttgtggga gataaagacg atccctacgc cagtgctcag
     9241 caattcacgc aggcggctga ataaacgatc ggtttccgca ggcgtcagtg aggcggtggg
     9301 ttcatcgagg atcaaaattc gtgaatcgcg catcagcccg cgcatgattt cgaccatttg
     9361 ccggtcggcc acatccagtg aacccgccgg gctgttgata tcaaactggc agcccagcgc
     9421 ggtcagcatc gccgccattt tttgcgccga gctctggtgt ttgggcagac caaagagaat
     9481 attctccttt accgtcaggc tggggaagag cagcggctct tgcgggacaa ggtagatgcc
     9541 taactgatgc gccaggatgg gcgtcagttt tgtacagcgg gctccctcaa tctcaatgat
     9601 tccgctatcc gcaggggtaa tgccggcgat gattttcatc agcgtggatt tcccggcgcc
     9661 attcccacca aggagcgcat gaacttcgcc tttgcataaa aaaaaatcaa ttcctttcag
     9721 gacgttgaca ccggaatact gcttgcagac agactgcgcg ctgagtagtg gggcgatctt
     9781 ttcttgactc atttgcatgc cacctccgtt cggcctaggt agaatcaggt tgaacaaatg
     9841 tattgtgcga ttcgaattgt tcataacctt atgacctaac tgctcataaa aacgacgaat
     9901 agaacacatt ttgtgataaa atgatcagcg aaataaaatt tattcaataa gaaaccacta
     9961 gaatgtggat ttaatcacag ttattaatga ccaatgaatg aacaattgcg ttcagaaatt
    10021 cgatatgttc aaagtgaaga caggatgatg acgattaacg acccgattat ttccgagcaa
    10081 gggatgtgtg aagaagagct tgtggcgcgc attgcgtggt tctattacca cgacggtatg
    10141 acgcaaagcg acatcagcaa cagactgggg ttgacgcggc ttaaggtatc gcgcctgctg
    10201 gaaaaggggc atcagtctgg gattattcgc gtacagatta attctcgctt tgaaggctgt
    10261 ctggaatatg aaaccgaatt acgtcgtcgc tttgacctga acaatgtgcg ggtcatcccg
    10321 ggacttcccg gcgcggatgt ctgcatccgc ttggggatcg gcgccgcgca tatgctgatg
    10381 gagtcactca agccgcagca gattctggcg gcgggcttcg gcgaagcgac catgaatacc
    10441 ttaaaacggc tcagcgggtt tatctcggct cagcaaattc gcctggtgac gctgtcgggt
    10501 ggtgtgggac cttacatgac cgggattgga caactcaacg cggcctgtcc ggtcagtatt
    10561 attccggcgc cgcttcgtgc ctcttctgcg gagattgcac agacgttaaa aaatgaaaat
    10621 agcgtccatg atgtcctgct ggccgcccag gctgcagatg cggccattgt cggtatcggc
    10681 gccatcaacc agaaagatga ggccaccatt attcgctcag gctatatcac gaagggtgaa
    10741 caactgatga ttgggcgcaa aggcgcggtg ggcgacattc tgggttactt cttcgatggt
    10801 gaaggagaag tgatcccgga gatgaagatt cacgatgaac tgattgggtt aacgctcagt
    10861 accctgaaga cgatcccgac tgtcattggc gtcgccgggg gggtgaataa agcggaagcg
    10921 atcgccgccg cgatgaaagg aggttatatc aacgcactgg tcaccgacca ggaaactgcc
    10981 gccgccattg tcagcgggca gtaatgcgta tctgccgtga aggtgcacgt aacagaatta
    11041 ccgtttgtac cgtggagaaa gcattgaaga cctgacagat ataataagcc gaggactaaa
    11101 atgatggccc aactctgtac cctctctgac tcagaacatt acctgatggc actggacgcc
    11161 ggaactggca gcgtccgcgc cgtgattttc gacctggaag gcaaacaaat tgcagtaggg
    11221 caagcggaat ggcagcactt tgccgtaccg gatgtgcccg gctcgatgga atttgatctg
    11281 ggaaaaaact ggcaactgac ctgtgaatgc attcgccagg cactgcataa cgccgggatt
    11341 gcaccgggag cgattgctgc cgtttccgcc tgttcgatgc gtgaaggcat tgtggtttat
    11401 gacgcagagg gaacgccggt ctgggcctgt gccaacgtgg atgccagagc agcacgggaa
    11461 gtcagcgagc tgaaagagat acacgactac accttcgaaa gcgaagtcta tcgcagcacc
    11521 ggccagactc tggcgttgag cgcaatacca cgtctgctct ggctggctca tcatcgttcg
    11581 ggcatctatc gtcgcgcctc gacggtcacg atgatcagcg actggatggc ctatatgctc
    11641 agcggcgagc tggcggtcga tccttccaat gccggcacca cgggtctgct ggatctcaaa
    11701 acgcgcgact ggaagccgtc actactgcaa atggccggac tgcggtcgga tattctctcg
    11761 cctgtcaaag agaccggcac gctgctgggt agggtgacgg cacaggctgc tgccctgtgt
    11821 gggctgaagg ccggcacgcc ggtggtggtg ggaggtggtg atgtgcagtt gggctgtctc
    11881 ggccttgggg ttgttcgtcc ggcgcaaacc gccgtacttg gaggaacatt ctggcaacag
    11941 gtcgtgaatt tacccacacc gattgttgat ccggaaatga acgtacgcgt taatccgcat
    12001 gtcatttcgg gaatggtcca gaccgaatcc atcagctttt ttaccggcct gacgatgcgc
    12061 tggttccgtg atgctttctg tgccgaagaa aagctaattg ccgagcgtct gggtgtcgat
    12121 gcctacacgc tactggaaga gatggcttca cgggtccccg caggtgcctg gggagtgatg
    12181 ccgatctttt ctgaccggat gcgcttcaag agctggtatc acgccgcccc ttctttcatc
    12241 aacctgtcca tcgacccgga aaaatgtaat aaggctaccc ttttccgcgc gctggaagag
    12301 aatgcggcca ttgtgtcgac ctgcaattta cagcaaatcg ctgattttac cggtatccat
    12361 cccacgtcac tggtgtttgc cgggggaggc tcgaagggca aactctggag ccagattctg
    12421 gcggacgtgt cagggctgac ggtcaacgtt ccggtcgtca aagaggcaac ggcgctgggc
    12481 tgtgcgattg ccgctggcgt gggggccggg atttggtcat ccatggacga aacgggcgaa
    12541 cggctggtgc gctgggagcg tgagcatgtt ccggacagag agaaacatgc gctttatcgg
    12601 gaatcgcgcg agaaatggca ggcggtatac caggaacagt taggtctggt cgatcacggg
    12661 ttgacgacct ctttgtggaa agcgccaggt ttataacagg agatcgtcat gattaaatcg
    12721 aatgaagcgc agcatgacta ccgttttggc gacagcggtc cgaaatacct cattcgcggc
    12781 ccgatgtgtg atatgggcgt cgtcatgctc cagcccggtc agtcttttcc caaccatcgg
    12841 cacatcgaag cgtgcgaaat tttttatacc ctggcaggcg aagtgacgct gtatctgaac
    12901 ggtgagcctt acacgctagg ctcgggggat gtgatgcact gcgatcccgg agaagcacat
    12961 tttctggtga acaaagggga agtgccgtgg aaaggagtct ttgtgaaatc gcctcatata
    13021 ccgggtgaca gtcatccggt tgagccaacg atgtattagt ttacgacata atacctgggc
    13081 tgtgagctgg cattttaacc agggttatgc gacagagtcg gttgctgcac tgtttgatta
    13141 tttatttaat gaaatcgtat tcccccggca gctttcgcct gtcaggggga agtcttattc
    13201 gctagcgtgc cattaacgca aagacacccc agccaaagta ttcacgtgta tatgagacat
    13261 gacgcatcgc ggacgtgcgc aatacgtcta cctttagcga gcacgtgtaa ggtacgttgt
    13321 tcctgacgag ataaattcat actggaaaac agatttcctc ctcatgctta tgcagcagcg
    13381 actggggaac ccatccatcc agcctgtccg tttgaagtac gctcacacca catccagcca
    13441 ttgagtgatt tcagcccgta aacctcatcc cctctcttca cctccagttc ccgggcgttg
    13501 taatcctccc ttgcaatgcc ccgatacgtc ccctcacatc tctcgaggat ctgtccgggc
    13561 acccagccgc cagtatgacc gggaattgtg cagtagaacc agttatccta gccttcctct
    13621 gaaccgcccc ggttttcctg gagagtgttt tatctgtgaa ctcaggctgc cagatcattg
    13681 tttccgatgg aagcataata agctttttct gcttctgccg gaggagtatg gcccagcctt
    13741 cccagcaatc gtcgattgtt ataccagtcc acccacgtga gtgtggccag ttccacttct
    13801 gcacggtttt tccagctctt acggtgtatt acctccgctt tgtaaagacc attgatgctc
    13861 tccgccatcg cgttgtcata cgagtcgcct gtacttcctg ttgatgccag taatccggct
    13921 tccttaagcc gctgtgtgta ggccagcgat acatactgag aacctttatc actgtgatgg
    13981 accgtgccgg acggtcgacg ggcccataac gcctgctcca gtgcatccag cacgaatgtc
    14041 gtttccatgg acgatgaggc ccgccacccc acgatgtatc cggcaaacac atcaatgatg
    14101 aacgccacat agacgaagcc ccgccatgtg ctgagtagag taagaggcag ggcgtagtcg
    14161 gactatttcc ctgcctctcc tccccgaacc ggacgtgcac ctttcagcgc atccggctct
    14221 ccatttaaat gctggcgaac gccattgcca cttctgtaaa gcgcgatgta tacgtgtttc
    14281 tggtctccgt cctcagatag ggattacctt cgggtagccg ccagcggaac agcttcttgc
    14341 cttgccccac caaccggtac agtatttcgc cgctgagctt gccgtgattg gttttaccaa
    14401 ataaaaccca cgttttgctc tgacccggtt tcggtgattt acaccaccac ctcatcaggg
    14461 aagcgatacc tgtacggtat ttgcgggcca gccagtgagc cagcttccag aacacgacac
    14521 ggtcgatata actgaagact ttggccttaa aatcaacgaa ctgatagaac atggcccagc
    14581 ctttcagttt tcggttgagt tgttcagcca tatcgacttt gctttcactg tagttgcctg
    14641 ataacagtgc tgtcagcgat gcggcgaagt ttctggcttt ctcctgcggg atcgttgaga
    14701 ccactcgcat ctcgccataa cgactgcgtt tgcgaatgat cctgtgcccc agaaagataa
    14761 agccgtcatt aacatgggtg attttagtct tatccatgtt cagcctgagt ttcagactgc
    14821 cttcgagcac accccgacac tcctccctga tggcttccgc ctgtgctttg gtgcctttga
    14881 cgatgaggac aaaatcatcg gcatagcggc agtacgccac cgcgggtttc cactgccagt
    14941 tttctctgac cgccgtactt cggccccgtt ggatactgtt attccagtac caccgatctt
    15001 ttctggcttt cccgctcagg tagcgctcat gcaggtattg atcgaactca ttcagcatga
    15061 tgttcgataa tagcggcgat ataacaccgc cctgtggcac accttcactg gccgcccgaa
    15121 agagaccgac atcgatatgt cccgccttga tggttttcca cagcagagtc atgaaacgtg
    15181 cgtcactgat cctgcggcgt acagccttca tcagcagtcg atgatgtacg gtgtcgaagt
    15241 aactggacag gtcgccttca atcacccagc gtccccgggt ttcaccacag tctgtgagct
    15301 gtaatttcac cgtgcggatc gcgtggtgga cactgcgctc aggccggaag ccatatgaga
    15361 gcgtatgaaa atcactctcc catatcggct ccatcgccat cagcatggcc cgctgaacaa
    15421 tacgatcccg caacgcgggg atacccagtg gtcgcagttt gccgttgctt ttagggatgt
    15481 aaacccgtct ggcgggcaag ggctggtagt ggcctgagag taattcatcc ctgaggattt
    15541 gcagctcaac agccagtctg gcctgtagca ttgttttgtt cacgccatca acgccggggg
    15601 tatgggcccc ctttgatgaa agcgtgatcc gcgccgcttc agccagccat tctggttgtg
    15661 ttatcagacg cagcagccgt tgaatccgta gggacggatc ggtgactgcc catgtggcaa
    15721 gcttgcgttg catttcgctg attatcaaag gtcttcacct cgttaggtca gttaattcac
    15781 gtcgcaaaca cattcaaact gcttcccttc gccatgtaat gggctttccc catcgcggac
    15841 tactacggaa gctccgccag ccagcgcgtc atcggagcca tgccccctta acatccgtcg
    15901 ctgaccttcc ccggtttacc tgcctggact caggcatact gaggaggctg cccgtcgcac
    15961 tctttatcct tgcttgccgc aagttggcag aagtcagcaa cgcaagcgtg atagacgctg
    16021 ctgccccggt gtttcgcata catgtcaaaa caccttcgac cggcagtgct tacgtatcac
    16081 tgccagttcc tcctgcacgg cctgtcagat cacgtaggcc gtggtgacgt tttcaaccca
    16141 cagaggcgga ttaacgggtt catgttcttc agcctttcag tacttaacct tgaggatcat
    16201 ctcggcttag tgatctcgcc tcaatccccg ttgtcagcgg gttacatcac cctgcgggca
    16261 tgccgcaggt cactgccgct caggttctcc accgtcacac ccggtgggat tgttgggttt
    16321 ctcatcgtga gttaccggct caatattcca gacagactcg cggttcattt aagcatccat
    16381 gcccgccctg aactccgggc acaccgtaag taaaatcagc cacccacagc tggtcaggtc
    16441 gttctgccac gaactgacgg tttacgcggt cgcctgcggc aacggctttc cggctgatgg
    16501 tcgtacggac ctttttaccc cggagaacac cggcaagtcc cataaccgcc atgagacgtg
    16561 ccacagtgca tctggccact ctgatacctt cccgtaacaa ctgacgccag actttacgca
    16621 caccgtatac cttgtgattt tcatcgtata cgcgctgtat ctctttcttc agccagtcat
    16681 cgcgctgcgc acgggcactg cgtttatccg gatgatgtcg ctgttgctga cagtggtaat
    16741 acgttgacgg ggcaatatgt agttcactgc ataccggtcc ggccccgtac agcttacgca
    16801 gcttatccag cagtggcatc actttttcca gaggcggtcg aactccgcct tcgcaaaata
    16861 agcggaagcc tggcgaagga tatcgttact gcggcgcagt tcacgatttt cacgttccag
    16921 ctctttcaga cgctgacgtt cagcggtggt gagccctcca tcaccgcccc cggtatcccg
    16981 ctcatgctgg cgaacccaga cacgcagagt ctccggcgta cagccaatct ttggagcaat
    17041 ggaacaaatt gtcgcccatt gtgagtcata ttcgctctga ctttccagaa ccatacggac
    17101 tgcccgttga cggacttcgg gggaaaaacg agtattttta gtcatcctgt ttacctcttt
    17161 ctcaggaagt ttagtctcca ggattcccgg ggcggttcac tgtcgtgatc tgtgattgag
    17221 gtcaacatgc tcagaatgtc ccctgtgaga ttgatgcgat acaggagaat gtcggcgtga
    17281 tttcagcgtc gtcctgctgg tgtgatatcg caaatatgcc aatgagataa cgggattcct
    17341 gattgcgtac accacgacga gcgcaggaaa aatcactgaa cgctctttga atctatcttc
    17401 gctgtcaggc caccaatgaa ccccgtgcca cattccgggg gcttctggtg ctgcgcgctt
    17461 cgtccagtga cacaaagcgc ttacattggc tatgacatcc atcatcaact gatccagaac
    17521 aggggcatcg aaatatgcct ctctgaccgt tatattccgg cgtatcggga gaaggtcaga
    17581 agtaccggtc atcagctcga gcaggatatt aatcaatgca gcccccgggt ttcatcctcc
    17641 tgaggcaggc aaaggctgag acgacgcccc cgtgtggttg attgacacca ccgtgcgact
    17701 tcgtcccacc actcacaatc aataccccgg gttttattca ggtaagcagg actgcggata
    17761 tccggtgtcg cgccgttgtg tcacgaacgg ggcaggagcg aaacaccgga taaaatacag
    17821 gttggcaata cctctgaacg ccctgcgcag agcggggatt ttggattaag tactcgcgcc
    17881 tccgcagtcc tgaaacaagt ctggctggta gctgtaaaca gacttcgtac atgttgctct
    17941 ggaatagatc cccgtgccac aggcttcgca gaactttttc ccgggaaaat gctgcccgca
    18001 catcacacaa tgccactcca gcacgaccgg taatggcgat agaaacatcg ccatatcctc
    18061 aatgtaaggg tgggactttt ccggattcag caccacgcag gccgccttct gttgcgcgct
    18121 cagggcatgt aaatcgtgct caaaccacgc cccctgagca tctgtctgca aaatcaaccg
    18181 accacgacag gaaaggcaga aacaatgcct gatatttctg ctaagggctg aggccgcact
    18241 gataatgtgt tcacccggcg tgatccccag ccccgttttt ataccgttca ttcagccact
    18301 ccctcctcac tgaagtgccc tgtatggcag tgagtgcagt accgctcccc ataataatcg
    18361 tggtgacatt gtctgcagtg ccagctggct ttacgcacca cgggtaaggc atccggtacg
    18421 aatttctgca gacgcttaat cagttgtatt tctctgcgct ccggtctgac ataagggcac
    18481 tgttgaccgt gctcagtcag cccgtcgtca gtgtgttcaa accagggaag ttcagtgtcg
    18541 tactgcggat ggtatctgag tgcactgccg caaaggtggc aggtgtagtg agcataaggt
    18601 gcggcctgtg cagtacgggc gcccgtcaga cgtccgttgc catcaagagc gataaaggat
    18661 tttgcgtaca tagtcatatt cctcagccgc cagacgacac gcaggcaaca gccgtcccgg
    18721 tacgggcagc gtggtcaggg tgtgaatggc ggttcagtta aaatctggac tggagtttgg
    18781 gtaaagcagg ctggttgctg ttacagcact gtggttgtca cacaatggcc caccggcgta
    18841 aaaaactgtc agcaggcagg gatggacatc gggggatgcg ctgtatcggt tgccggaagg
    18901 aatggtaaag aatgtgtcat ggttaaatct ccgtatggtt catacggaat ggccacgccg
    18961 taaaaaatgc gcagagccgc tgaaatggcc gccggaaccg acagcaaaga ttagcgccag
    19021 cccgtacttg tcgttcaggg gcttacacca gagcacatat ccccggcagg gggctgtcca
    19081 gtcgtggtcg accccggtta cttttttcag gaagcccgct gcatttcagg atgatgccac
    19141 attctttctg ttcccgtttg gccaggtagc tgatggcata gcgtaaagca ttcaccgctt
    19201 tgtgatctga atagtgaatg acgtggttaa tgtttaccgg ataattttta ttaaaccggc
    19261 accagtggta gtaaccatta ccctcggtaa cccgcctcca gatatctccc atcaatctgg
    19321 ataccagata cgaactcctg tggctctggc cgttcagata gcccacaaaa tgaatgtgaa
    19381 gccctttacg ttctgtatat tccatcaccc aggcataccc gaccagtttc tctcccacct
    19441 cgttaatcag ttgcagcatc tcagcgcaca tggcgtacct gcaggcacat ctgtagctga
    19501 gggtgttttt tcggtaagca aaatccattc tgaacggtaa cagtttcgaa tagaccccga
    19561 ataatgaatc cagatgctgg ttaaagtgtg tcaggagctg gggatccatt tcgtaaccgg
    19621 gattggcatt gtaagtattc atgtttcttc ctcgtgttct gtaacacagg aagaagcgct
    19681 ggcgtaattt ttgaagaaaa cgttatcgca cggagacgtg caagttcaca actccccggg
    19741 cgccacagag gaagtgagac cacacccggg tatcagaacc atacagagaa cggcatacgt
    19801 gactgtcaat cacagcgcag ataacttctg aagtgatgtc attgtgacga ctgtacttca
    19861 tcagaaacat ttatcaggta acatatttcc agactgaagc gggaacacga tcgtaaagtt
    19921 tgttcatggt cagttctgcc agccggtgat cgaccgcaga gttgaaggct tccatctcct
    19981 ctggagtaag cttataacgc gcgtgggaaa tcactttttc cagtgaacaa cagtggaact
    20041 actctggttt aaaattccat taccctctcg ttatcttgta ctgtgattaa cgtaatatcg
    20101 tcataatggc ctgacgttat cccgaaagcc tttcc
//
LOCUS       DAFNBJ010000086        20126 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000086 DAFNBJ010000000
VERSION     DAFNBJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.086"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(167..913)
                     /gene="cutC"
                     /locus_tag="L2X49_002947"
     CDS             complement(167..913)
                     /gene="cutC"
                     /locus_tag="L2X49_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBN4797997.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(927..1493)
                     /locus_tag="L2X49_002948"
     CDS             complement(927..1493)
                     /locus_tag="L2X49_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBN4797998.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1709..3442
                     /gene="argS"
                     /locus_tag="L2X49_002949"
     CDS             1709..3442
                     /gene="argS"
                     /locus_tag="L2X49_002949"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBN4797999.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLADHVQQALAS
                     DRLDVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3619..4107
                     /locus_tag="L2X49_002950"
     CDS             3619..4107
                     /locus_tag="L2X49_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBN4798000.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4227..4619)
                     /gene="flhe"
                     /locus_tag="L2X49_002951"
     CDS             complement(4227..4619)
                     /gene="flhe"
                     /locus_tag="L2X49_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBN4798001.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4619..6697)
                     /gene="flhA"
                     /locus_tag="L2X49_002952"
     CDS             complement(4619..6697)
                     /gene="flhA"
                     /locus_tag="L2X49_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBN4798002.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6690..7838)
                     /gene="flhB"
                     /locus_tag="L2X49_002953"
     CDS             complement(6690..7838)
                     /gene="flhB"
                     /locus_tag="L2X49_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBN4798003.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8040..8684)
                     /gene="cheZ"
                     /locus_tag="L2X49_002954"
     CDS             complement(8040..8684)
                     /gene="cheZ"
                     /locus_tag="L2X49_002954"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBN4798004.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8695..9084)
                     /gene="cheY"
                     /locus_tag="L2X49_002955"
     CDS             complement(8695..9084)
                     /gene="cheY"
                     /locus_tag="L2X49_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBN4798005.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9099..10148)
                     /gene="cheB"
                     /locus_tag="L2X49_002956"
     CDS             complement(9099..10148)
                     /gene="cheB"
                     /locus_tag="L2X49_002956"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBN4798006.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10151..11011)
                     /gene="cheR"
                     /locus_tag="L2X49_002957"
     CDS             complement(10151..11011)
                     /gene="cheR"
                     /locus_tag="L2X49_002957"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBN4798007.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11030..12631)
                     /gene="tap"
                     /locus_tag="L2X49_002958"
     CDS             complement(11030..12631)
                     /gene="tap"
                     /locus_tag="L2X49_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBN4798008.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12677..14338)
                     /gene="tar"
                     /locus_tag="L2X49_002959"
     CDS             complement(12677..14338)
                     /gene="tar"
                     /locus_tag="L2X49_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBN4798009.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPLLRIAEQDPNWETF"
     gene            complement(14483..14986)
                     /gene="cheW"
                     /locus_tag="L2X49_002960"
     CDS             complement(14483..14986)
                     /gene="cheW"
                     /locus_tag="L2X49_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBN4798010.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15007..16971)
                     /gene="cheA"
                     /locus_tag="L2X49_002961"
     CDS             complement(15007..16971)
                     /gene="cheA"
                     /locus_tag="L2X49_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBN4798011.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQNRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSFSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLISQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16976..17902)
                     /gene="motB"
                     /locus_tag="L2X49_002962"
     CDS             complement(16976..17902)
                     /gene="motB"
                     /locus_tag="L2X49_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBN4798012.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17899..18786)
                     /gene="motA"
                     /locus_tag="L2X49_002963"
     CDS             complement(17899..18786)
                     /gene="motA"
                     /locus_tag="L2X49_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBN4798013.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18913..19491)
                     /gene="flhC"
                     /locus_tag="L2X49_002964"
     CDS             complement(18913..19491)
                     /gene="flhC"
                     /locus_tag="L2X49_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBN4798014.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19494..19844)
                     /gene="flhD"
                     /locus_tag="L2X49_002965"
     CDS             complement(19494..19844)
                     /gene="flhD"
                     /locus_tag="L2X49_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBN4798015.1"
                     /translation="MHISELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4811 a         5551 c         4911 g         4853 t
ORIGIN      
        1 aaaaaatatt attagcacaa cgctatgttc actgtcaccc gctcaacaga tcacggttat
       61 cgttcgtttt ttatactgct cagggataaa aaaaggcccc tgttgaaatt gcaggggcct
      121 ggtacgagca agcatcatat tgggcgacat gatgcaacgg taaaaatcat ttggcctgat
      181 ggcgttcaat gattcctttc atttcagcaa ccgccgcccc gtctacgata taacgcgaat
      241 actcgtccgc gtgttcatct gatgacatgg acaatccttg attacgataa cgcatcggtg
      301 aggcttgcca cgctcccgcg gagctatgga cttccagcac tccggcatcg aggaagtggt
      361 gcaggttttc tgcacggact cctgctccgg ccataatgat tggagcatca cgatgggcaa
      421 taagttccat aatttttgat aaaccttgca gtgcgtctga tttttgccct gatgtcagta
      481 cccgggcaat gcccaattcc gcaagattat tgagtgtata taaagggtta gcgcacatat
      541 cgaaggcgcg atgaaaagtc actgccagcg gaccggcagc agccattatt ttttccattc
      601 gtggcatatc gacattcccg tcaacatcga gaacgcccgt caccagtcca ggaaaaccta
      661 attcgcggac cgtgcgcaca tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa
      721 aatcaccacc gcgtgggcga attatcggat gcacagggat cgtcacccgc tggcgcacgg
      781 atttcagtac ccccaacgac ggcgttaagc ccccctcttt tggggctgcg cataattcaa
      841 ctctgtctgc gccgttttgc tgcgccgtta gtgcacattc catgctgtaa cagcaaattt
      901 ccagtaatgc catttttgct ccttaattac gctgactgct cgctggcaac gatctcttca
      961 atggaccacg gatgaaactt aatagttgtt ttgccatcgg tgaccgccag cgtcggattc
     1021 ggtaatcgct catgctcacc ttttggtgaa ctggttttaa ctgagatccc cggtaggctg
     1081 agtccttcat cagaaagcaa agccagcgca cgggcattca gcgtttctgg atcacccggc
     1141 agaacgattt caatatgttc ccaaccttcg tgtgggtaac gtttttcccc gggccacggt
     1201 agctccacaa tagaaaactg ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga
     1261 caaatcggtc tgccattgat catattttct gacaaaagct cgccacactg ttcaaacccg
     1321 cgacgccagc gctcagcggt gacgttttga tggcaacgca aagaaatgtg atcggcagtc
     1381 agcggagtga tattcaaccc cagacggcgg gaaagttcat ctaatgcgtg ggtaaatcgc
     1441 ggtaaatccg atgcaatatc ctgcagctca tcgatagatt gccagttcgc cataatcact
     1501 cttcgtcttt cagtaaaagc gttaatttac cctgttgccc tgtgtcaacc aaccgctgat
     1561 ttcacgccgc ttctgatgca atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg
     1621 aaaacagcca tttgcagtat actcccgccc taatttcttt aactggtgcg ggcaattttt
     1681 gctcgcttca tcaatgtaag gtattccggt gaatattcag gctcttctct cagaaaaagt
     1741 ccgtcaggcc atgattgcgg caggcgcgcc tgcggattgc gaaccgcagg ttcgtcagtc
     1801 agcaaaagtt cagttcggcg actatcaggc taacggcatg atggcagttg ctaaaaaact
     1861 gggtatggca ccgcgacaat tagcagagca ggtgctgact catctggatc ttaacggtat
     1921 cgccagcaaa gttgagatcg ccggtccagg ctttatcaac attttccttg atccggcatt
     1981 cctggctgat catgttcagc aggcgctggc gtccgatcgt ctcgatgttg ctacgccaga
     2041 aaaacagacc attgtggttg actactctgc gccaaacgtg gcgaaagaga tgcacgtcgg
     2101 tcacctgcgc tctaccatta ttggtgacgc agcagtgcgt actctggagt tcctcggtca
     2161 caaagtgatt cgcgcaaacc acgtcggcga ctggggcact cagttcggta tgctgattgc
     2221 atggctggaa aagcagcagc aggaaaacgc cggtgaaatg gagctggctg accttgaagg
     2281 tttctaccgc gatgcgaaaa agcattacga cgaagatgaa gagtttgccg agcgtgcacg
     2341 taactacgtg gtaaaactgc aaagcggtga cgaatatttc cgcgagatgt ggcgcaaact
     2401 ggtcgacatc accatgacgc agaaccagat cacctacgat cgtctcaacg tgacgctgac
     2461 ccgtgatgac gtgatgggcg aaagcctcta caacccaatg ctgccaggaa ttgtggcgga
     2521 tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg accgtcgtat tccttgatga
     2581 gtttaaaaac aaggaaggcg aaccgatggg cgtgatcatt cagaaaaaag atggcggcta
     2641 tctctacacc accactgata tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga
     2701 tcgcgtgctg tattacatcg actcccgtca gcatcaacac ctgatgcagg catgggcgat
     2761 cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg gaacaccaca tgttcggcat
     2821 gatgctgggt aaagacggca aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc
     2881 cgatctgctg gatgaagccc tggaacgtgc acgccgtctg gtggcagaaa agaacccgga
     2941 tatgccagct gacgagctgg aaaaactggc taacgcggtt ggtattggtg cggtgaaata
     3001 tgcggatctc tccaaaaacc gcaccacgga ctacatcttc gactgggaca acatgctggc
     3061 gtttgagggt aataccgcgc catacatgca gtatgcttac acgcgtgtat tgtccgtgtt
     3121 ccgtaaggca gaaattaacg aagagcaact ggctgcagct ccggtaatca tccgtgaaga
     3181 tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc
     3241 ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac gatctggctg gtctgttctc
     3301 tggcttctac gagcactgcc cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg
     3361 tctgaaactg gcacaactga cagcgaagac gctaaagctg ggtctggata cgctgggtat
     3421 tgagacagta gagcgtatgt aatcgatttt tcgtgagagt aaagcctgat cagggttacc
     3481 tgatcaggct tttttattgc catctaaatg tattctgaaa atggacatgc cattgttttc
     3541 tgactgttgg ataagaggcc agaagcgtaa tatccggccc cagggaaacg ataacggttg
     3601 aatttaagga ataccgcagt gtttaaattt cttgtattaa cattaggcat tatctcttgc
     3661 caggcttacg cagaagatac ggttatagta aacgaccatg acatttcagc catcaaagat
     3721 tgttggcaaa aaaattcaga tgatgatact gacattaacg tgatcaaatc atgcctgcga
     3781 caagaataca atctcgtcga tgcgcaatta aataaagcct atggtgaagc ttatcgttat
     3841 atagaacaag tgccacgcac aggtgtaaaa aaacctgata ccgaacaact taacttgctt
     3901 aaaaaatcac agcgagcctg gctggatttt agggacaaag aatgtgaatt aatcctttca
     3961 aatgaggacg ttcaggattt aagtgaccct tattctgaat cagaatggct ctcatgtatg
     4021 atcatacaga ccaatacgcg aactcgccag ttgcagctat accgtaactc tgaagatttt
     4081 tatccaagcc ctttgacaag aggataattc acatcttttt ggcatgtttt gttgcaagct
     4141 attcctgata aataattgca acaagacatc gagccttttt cactgagtta ttaaacatac
     4201 tcgcgagcgc gtaatttttt tgtccttcag cgataattca caatcacttc attacgttgt
     4261 acctttagcg gtggaattaa ccgcccaccg cctggcactt cccagataaa tcgcaacggt
     4321 tctgctgccg ctataccgga aaaggccacg gtggttccgc tctgcccctc taattcgaca
     4381 caacgagatt gcgaacacaa gcgaacccgc agtcctgaag gtgtcgggcc gataagctga
     4441 taacgccacg ctaccagcgt catcaatcct gaagcaggtt gtcgcgtaga aagaggcgca
     4501 gacgacatcg actcaccgcg atgatttagc gtaattccta cactacttgc ctgccacatc
     4561 ccctccccgg cggcttgcac cagcagcgga aacaataata ttgctaataa ggttctcatt
     4621 atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt atcagacagt tccagattcg
     4681 acaggaccac taactgcggc aagctgcggc gcaggaagcg agacaataat ggtcgcagcg
     4741 cgtggttcac caacaatact ggcggcgcac ccagcatctc ctgacgggat agcgcttcct
     4801 gagtttgcgc cagtaaacga tccgccagcc ctggctccag tcctcccccg ccctgcagcg
     4861 cctgtagtaa caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgc
     4921 caggaaacca ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt
     4981 catgtggatc gctttggatg ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat
     5041 cgcgaatcgg tactttttca tcgaggagat tttgcagcac tttatgcagt gtggtgagcg
     5101 tgacgacgcc aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca
     5161 acagctgttg cgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt
     5221 taagatgcgt tgctaccacc gtgctggcct caaccactgt gtacccctga atctgcgcct
     5281 gttcttttag cgcactttca atccagatag cattcaggcc aaatgccgga tcgacggtcg
     5341 cctcaccagg taacgtcccg gcagcggttc cagggttaat cgccagccag cgccccggat
     5401 aagcatcacc actgccaatc tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca
     5461 gatccatatt gtcgcgaatg tgcaccactg gcggcagaaa tcccatctcc tgggcaaatt
     5521 tcttgcggat actgcgtata cggcccaaca actcaccatc ctgctggaaa tcgaccatcg
     5581 ggatcagtcg ataacccact tccattccca gagaatcttc cagttgtaca tcgttccacg
     5641 tcgcttcgac aacggtatta ttctctgcca tttttaccgg tttgggttcg gcaggcgctt
     5701 tttgttcgcg tccgcgtatc caccaggcca gccccagcaa tccggcagtg aacagcaaaa
     5761 ataccaggtt cggcattcca ggcaccaggc cgagtaaacc gagcacggcg gcgcttaaca
     5821 acataacgct tgggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg
     5881 tgctgacacg cgtaacgatg acccccgcgg cggtagaaat caccagcgcc ggaatttgtg
     5941 ccaccagacc gtcgccaatg gtcaatagcg tataactttc cgccgcgtgt cccatgctca
     6001 tgccatgttg cagcacgccg accagcaacc cgccgacaac gttaatgacc atgatgagga
     6061 tcccggcgat ggcatcgccg cgaacaaact tacttgcccc gtccattgag ccgtaaaaat
     6121 cggcttcctg agtcacttcg gagcggcgtt ttttcgcctc atcttcacca atcaatccag
     6181 cgttaaggtc ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg
     6241 cacccacttc tgcgatacgc ccggcacctt tggtaatgac cataaagttg atgatcacga
     6301 gaatgacaaa caccacgata cctatagcga aattgccacc aacgaggaag tgaccgaacg
     6361 cttcgaccac cttccctgcc gccgccgcgc cggtatgccc ttccatcaaa atgatacggg
     6421 ttgaagccac gttaagtgcc agacgcaaca acgtagtaaa caacagaatg gtcggaaacg
     6481 cagcaaactc aagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg
     6541 caatattgaa ggtaaacaac aggtcgagta tgaatgcggg cagtggcagc accatcatcg
     6601 acaagatcaa caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt
     6661 ttgcgggcag gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa
     6721 tccagggctt ccggcaccgg aagatgagta ggttgtacag ggcgctgtcc accagccaga
     6781 cgccagcgtt tcagttgcca gacccaggcc agcacttccg ccaccgcggc gtacagttga
     6841 cccgggattt gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct
     6901 tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct
     6961 gcacctttag cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc
     7021 gaatagtggg tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga
     7081 cgccgtgcag cagctcgctg catctgacgg atccgccctt taacatgagg gtcaccttcg
     7141 ctttgtttga actcatcacg aatatcctgc cgtgacatac gcagcttttt caggtggctg
     7201 aatatttgga aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt
     7261 gcgcatagcc ctaccaaatc catcgcatta cccatggcgg taatcggaga ctcggccatc
     7321 aagcgcatca tctgcggcca gtgatgccag agaaaaaacc ccgtcacgct gccaaccagg
     7381 atggttttca aaattgcttt aagcaactcc gcgccagtct gagccgagaa catccgttta
     7441 atgcccggta gcgggttgag tttggaaaac ttcggctgca aggatttgcc gctaaatacc
     7501 agccctccca gcatgaccgg agaaatgagc gccaccagca ccacgccgct aatcagcggc
     7561 agcagcgcca gcatggcttc tctgatcagc agaataatct gcccgaggat cagattcggg
     7621 tcattgataa tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg
     7681 gccagcgaca caccgccaaa ccagataaca ctaacgccca ccagcaaaat cagcagtgag
     7741 gtcagttcac gggaacgcgg gatttgcccc tcttcccgcg ctttttctag tcggtgaggt
     7801 gtgggggctt ctgttttgtc gtcgctctcg tcagacacgt cgccaatcct ggaaagagta
     7861 ttaagcgtga atgatgccag agcgcaaagc gttcaatggt ttgagtaagg ggcaaaacag
     7921 gcgggattta gggcttttgc tgccacacat caagcatagt gtgcgtttgt cggatgcggc
     7981 gtcatcgcct tatcagaccg cctgatataa cgtggtcacg ccacatcagg caatacaaat
     8041 caaaatccaa gactatccaa caaatcgtcc acctgatcct gactggctac cacaccggct
     8101 ttgctggtat cgacctgagg tccattaagc aaactctggt tttcacgttt tggacgcgac
     8161 tcctgttccg ggatgttttc caacagcacc atcagcaact ggcgttcgat ctcctgaatg
     8221 acatccatca tccgcttaat aacctgtccg gtgagatcct gaaaatcctg cgccatcatg
     8281 atttccagca gttgcgcgtt tgtaaagctg gtatgcgcgg gtacatctgc cagaaattgc
     8341 cgcgtgtctg ttaccagttc acgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca
     8401 tcccaacgtt gggttaacgc ttttgctgat ttctccattt gatcctgatg cggttgtgac
     8461 gcctcaacac tgttcagcgc ccgctccgca gcctgggcgg tcatctgcac aacatagtac
     8521 aaacgatcgc gcgcatcggg gatggcttcc gccgcttcgg caatggcctg atccagcccc
     8581 agttcccgca aactgtcgcg cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg
     8641 ccagctgaat gctcgtcagc aggtttgatt gatggttgca tcatagtcgc atcctcacat
     8701 gcccagtttc tcaaagattt tgttgagttt ttcctccagc gtcgcggcgg taaatggctt
     8761 caccacatag ccactggccc ccgcttgcgc cgcagcaatg atgttctctt tcttcgcttc
     8821 tgcagtcacc attaacactg gcaatgccga catcgcgcca tccgcacgaa ttgttttcag
     8881 caattccagg ccatccatat tgggcatgtt ccagtcggag ataacaaatc cataaccgcc
     8941 tgcctgcaac ttattgagag cgtcgacgcc atcttccgct tcctcaacat tattgaatcc
     9001 cagctctttc agcaggttac gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa
     9061 aaatttaagt tctttatccg ccatttcaca ctcctgattt aaatacgtat cgcctgtccg
     9121 gcactaattt ttgccaacat ttgctggctt acctggctaa gatcgaccac ttcgcagaca
     9181 ccacccatat tgatggcctc gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgc
     9241 gcaagggtcc atgcccccgc ctgacgcatc gccaacattc ctgccgcgcc gtcgttgccc
     9301 ataccggtca ggatcacccc aaccgcatta cgccccgcct gtttggcgac agaatggaac
     9361 aacacatcta ccgaaggccg atgacggtta accgccgggc catcatgaat tttgatttgg
     9421 taatttgcgc cactacgcgc cagctccata tgccgatcgc ccggcgcgat ataggcatgc
     9481 cccggcaaaa cacgttctcc gtcttcggct tctttaaccc cgatctgaca aagcttatta
     9541 agtctgtcgg caaaagagcg ggtgaaacca ggcggcatat gctgggtaat taacagtgcc
     9601 gggctggaaa gcggcaacgg ttgcagtacg tgacgaattg cctcagttcc acccgttgaa
     9661 gcaccaatcg caatcagttt ttcagaactc aacaacggcc ctgccttcag cgttgtcggt
     9721 gccgacaatg gcttatgtgc tgcaaggctc gcctttgctg ctgtacgcac cttttcagca
     9781 atcatttcgc tatacgccag cattccttcg cgaataccca gttgcggttt ggtgacaaaa
     9841 tctatcgccc ccagctccag cgcgcgcagc gtgacttctg accctttgcc ggtcagggaa
     9901 gaaaccatca ctacgggcat tggacgcaaa cgcattaatt tttcgaggaa atccagtccg
     9961 tccatccgcg gcatttcaac atccagcgtc agcacatcgg gattgaattt cttaatcaag
    10021 tcacgcgcga ccagcggatc aggcgcggtc gccaccattt ccatgtcgct atggctgttg
    10081 atgatttctg tcatgatctg gcgcatcagt gccgaatcat cgacagataa caccctgatt
    10141 ttgctcatcg ttaatcctta cttagcgcat acaccgtctg accacgcagc gtgaagcggc
    10201 gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag caatccgtcg ggtttaagga
    10261 gcggaacaaa gcggcgcaaa atctcctgct gggtattttg atcgaagtag atcatgacgt
    10321 tacgacagaa gatcgcatca aacggccccg gcacggtgta ctgtttcgcc agtagattca
    10381 gcggggcaaa atcaacatag ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg
    10441 gtcccgtccc tcgcatgaaa taccgttgca gttgctgcgg cgtcaggttt ttcaactctt
    10501 catggcgata aataccgctt ctggcttttt ccagcacttc ggtgtcgata tcactggcaa
    10561 acactttcca gcgtccgggc gcggtgccca atgtgtcagc cagcgtcatc gcaatgctgt
    10621 acggctcttc gccggtcgaa gccgccgcgc tccatacgcg atactcgcca gaacggcgac
    10681 gtgcgtgatc cgcgagcaga gggaaatggt gtgcctcacg gaaaaatgcc gtcagattcg
    10741 tggtcagcga attgataaac gcctgccact caccgctgtg ctgattagat tccagcaagt
    10801 tcagataatg accgaaatcc gtcagtccca gcgaacgcaa acgacgaacc agtcggttgt
    10861 aaaccatgtc gcgtttatgg tcagccagaa cgatcccggc tcgttgatag atcaattgac
    10921 ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag
    10981 acgtttgccc acagggcaga gatgaagtca tagcgccttc tcaatcactt caggatacca
    11041 ctggcgcaat ttgtaactgc gccgactcat gtcgtgctac ttcatgttct tcaagggtaa
    11101 ataccgccac acgcgacgaa agatggtcgg cctgattcgc cagttgttcc gttgccaccg
    11161 ccgcttcttc taccagcgag gcgttctgct gagtcacctg atccatctgg ctgacagcct
    11221 gtgcaacctg ctcaatcccc cgctgttgtt cttccgacgc agaggctatt tctcccataa
    11281 tgtcgttcac gcgggtcacc gaactgacaa tatcgatcat ggtcgcggcg gcgttattca
    11341 ccagtttcga accctgctgg acacgattga ctgactcttc gatcagccct ttgatctctt
    11401 ttgctgcctg cgcgctacgg ctggcaagat tgcgcacttc acctgccact accgcaaaac
    11461 cacgcccctg ctctccggcg cgagccgctt ccactgccgc attcagggcc agaatattgg
    11521 tctggaaagc aattccgtcg ataacgctga taatgtcgcc aattttttgc gagctggtgg
    11581 cgatctcctg catggtgtga gtcatggtac tgacctgaac accgcccgcc tgcgccgttg
    11641 tcgcggcatt ttttgccagt tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg
    11701 tggcggttaa ttgctccata ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg
    11761 tacgacttga gagatcgtta ttgcctgcga caatctccgc gataccaatg tgcatctcct
    11821 ggcttccctt acgcacatca cttaccgtcc cacgcaaagc ctgctgcatg gtcttcagac
    11881 tggcaaaaat ggcggtgatc tcattacgac catataccgc aatcggacgc gccagattac
    11941 ccgcagcaat gctgtcaaaa tggctaccga taatggccag tggttgaaca atcatcttgc
    12001 gcgtccacca cagcgcactg ctgatgtaga tcgccgcaac aataatcatg ctgataaaca
    12061 ccagtgccga aatctgatag ttacgctggc tttgcgcact ggcggcttcc agcacatggt
    12121 tgatctccag ctgccaggcc tcaaagttaa cgtcaaacgc attctgtgat ccctgcaccg
    12181 gcgcagtgag gaaatccgaa agttgattac tttcaagcca ggtcgcctga tgttcgagat
    12241 cgttgtgcca gcgggcaaaa cttttctccg tctctttttg caatgccctg acgtgctcgt
    12301 tgcccgcagt catcgccata aaacttttaa acagcgtggt ggattgcgtc agactggcgc
    12361 gcgccgtcgt catcaacgtt ttaatgtcat ccgccggata gctaagtgcc gtcagagtgc
    12421 ccgctttgtt cagcgcggta ctggcctgta acattaccgc ccgagtttgc gctaatgccg
    12481 cacgttgctg attgctctgc tcgacctgat tcagtcgttg caaatcgtcg cgaaatgccc
    12541 aaaaagacat gccgttactg ccaatctgca agatcccgca gagaatcaaa attaaaaaca
    12601 gcgtggtcga aattcgaata cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg
    12661 cgttagtaaa tactcgtcaa aatgtttccc agtttggatc ttgttcagca attcgcagta
    12721 gtggctgtgc cggtggttgc tcactggcag gacgggatgg tgtttgcggt ttattggtga
    12781 gtgggctggc tgccagacgg aacgcggaaa ccgcttgcgt taaacgactc gcctgttctt
    12841 ccagcgcagc ggcggcggca gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc
    12901 gatccatttc cgaaaccgcc aatgcgactt gatcgatgcc acggctctgt tcatccgatg
    12961 ccgatgcaat ctcgcccata atgtcagtca cgcgagtgac agcattgacg atattgttca
    13021 ttgtttcccc ggcgctttcg accagcaccg aaccggtatc aacgcgtgag acggagtctt
    13081 caatgagggc tttgatctct tttgccgcct gggcgctgcg actggcaaga ttacgcactt
    13141 cacccgccac cacggcaaaa ccacggccct gttcacccgc acgcgcggct tcgaccgcgg
    13201 cattcagcgc gaggatatta gtctggaagg caataccgtc gataacgctg ataatgtcgg
    13261 caattttctt cgaactatcg gcgatctcat gcatcgtttt cactacgcca tccaccactt
    13321 tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt
    13381 tatcggcgtt ttgcttcact gtcgcggtga gctgctccat gctggcggca gtttcttcca
    13441 gcgcggatgc ctgctgttca gtacgggagg aaagatcggt gttgcccgcc gcaatttcac
    13501 gggtaccggc atagatggca tctgaacctt cgcggacatg agtgacggtg tcagtcaaag
    13561 agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg
    13621 tcagggtatt cgccaggtta ccaccggcga tttcgcgaat gtgagcaata atttttgcca
    13681 gcggagtaag caacatacgg cgaatgccgt accacgccac cagcagaatc aataccacca
    13741 ccagcgcgat aaccgccagt tgccactggg caaatcggta atcatctgcg ttgtcagtga
    13801 cgatatcgcg atacagtttt tcactgctga gggcgtactg agcaaacgct tcgcccattg
    13861 cattttgcat tccctgggtt ggctgagcga aataagctcc agtattgcca taatctagat
    13921 aatcaatcag ttcagttaac gctgtgtaat agtttttata tttttcatca atattacgac
    13981 tggtagcgac catttcaggt aacggtgcca tgcttttgaa ttttttataa tgcgtcgctg
    14041 cctgcgccaa tgttttcctg gcgctatcga gcaattcaac tttggcgtta ctttgttgat
    14101 tggaggaatc catcatcatc cgcaccgctg aacgactcag gttaatgcgc gtttgcagca
    14161 ttaaatccca ggttgacgtc agctcgccct gctgttcccg caattgattg gaaaccacaa
    14221 agctcttctg gctatggtga agggaagaaa aaaacagact gccggaaata agctgtaaca
    14281 gtgcgaatac ccccagcacc attaccaaca gcgtgactac gcggatacgg ttaatcataa
    14341 ggcaccttcc tgaaaacaag ttgatctcgt tatcggcaag aaggggggaa actttattgc
    14401 tgatgccacc cgccgcgaaa ttgaaataaa aaacccgatg cgcagatcat cgggttcatt
    14461 tcaattgagg aaatcgggag aattacgcca cttctgacgc cgcgctatct aacagcgcca
    14521 tctcttcgct gttcagcagt ttttcgatgt tcaccagaat caacatacgg tcgcccagtg
    14581 cgcccagtcc agtgagatat tctgttgaaa gcgtcacggc aaactctggt gccggacgaa
    14641 tttgctccgc cgtcaatgaa agcacgtctg aaacgccgtc aaccacgatg ccgaccaccc
    14701 gctgtccgag attcaggacg ataactaccg tgttgtcgtt atagtccaca tccacctggc
    14761 tgaacttaat tcgtaagtca acaatcggca caataacgcc gcgcagattc gtgacgcctt
    14821 tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc gtagccacgg atctcctgca
    14881 ctttcaggat atcaataccg tactcttcat caccaagggt aaataccaga aattcctggc
    14941 ctgacggctc gctggccagc tttgttacat tcgtcatacc ggtcatattg ttaccttttt
    15001 actcattcag gcggcggtgt tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga
    15061 gacatcaaca atcagtgcca cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg
    15121 gactttgcga tagttacttt caaggttttt aaccacaacc tggtgttgac taattaattg
    15181 atccaccagc aaggcgtagc ggcgaccgcc actttgtaag atcacaacaa ttccctgggt
    15241 ggcttcggtt ttcgcgcccg cgacgttgaa cactttccac agttcgacga tgggcagata
    15301 ttcaccccgc acttccagca cccgctcgcc gccggccagt ggatggagat cggcttcacg
    15361 gggttgcagt gattccataa cagcattcag cggcagaatg aaaacttcat ccgcaacgcg
    15421 tacggacatg ccgtcgagga tggccagcgt cagcggcagt aaaatgcgga tcgtagtgcc
    15481 agtcccctgc ttcgactgga tttcgacatg accgcccatc tcctggatat tacgtttaac
    15541 gacgtccatg ccgacgccgc gcccggagac gtcggtgacc tgctctgccg tggagaagcc
    15601 tggtgcaaat atcagcatcg cgacttcgtc gtcgctcatg ttttcgctga cggtcagccc
    15661 ttgcgaggcc gcttttgcca gaattcgttc acggtttagc cccgccccat cgtcggtcac
    15721 ttcaatgcaa atgttgccgc cctgatgttc ggcagaaaga attaaatttc ctacgctgtt
    15781 tttacctgcg gcgagccgtt tttctggcag ttcaataccg tgatcgaggc tattgcgtac
    15841 caggtgggtc agcgggtcga taatgcgttc aatcaggctc ttgtcgagtt cagtggaact
    15901 gcccaccagc gtcagttcta cctgcttgcc gagttttccc gccagatcac gcaccagccg
    15961 cggataacgg ctaaagacat attccatcgg catcatgcga atcgacatca ctgattcctg
    16021 caaatcacga gcgttacgtt gtaactgccc catgctggta atcaaatcac catgattaac
    16081 cgggtccagt tcgctggaac gctgggcaag catggactgg gtgataacca gctcgccgac
    16141 gaggttaatt aattgatcaa ccttttctac cgctacacgg atgctggtgg attcactgct
    16201 acgtgtcgtt ttttcccgct ccacgcgacc ggttggcgct tgttcggctg ccagtttaag
    16261 cactggtggg gtggatattt ttggcgagac ttctactgtt tcaaaggtaa tctgatcggc
    16321 ttcaatcaca aaacaaagta ccgctgtgat gtcatcttcg gcaatgtcgc ccggtaatat
    16381 tgccgagaaa gaatccgccc ctttcaccac atcagttaac gttgtcagat gtcccagctc
    16441 ttcttccagc aggtcgactt cgccggcctt caggcgcgaa aggataattc gtcgcggcga
    16501 ctgactgcga ttctgctcat cttgcggttc acttttggca accacactta atcgggtcac
    16561 tgcggatggc gtttcgcctt tcgcttctaa tgccagttga cgcaaggcct ggcagatata
    16621 atcgaagctg gcggcatccg gctcttgcga ctgtttataa gcgtcgagct gttcttgcat
    16681 gatgtccttc gtttccaaaa acagattgat aatgtcggtg ttgagttgca tctcacctcg
    16741 tctggcttca tcgagcaggt tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc
    16801 aaaagttcct gcccctcctt tgatcgagtg ggcagcccga aagatggcat tcaattgttc
    16861 ggcatctggc gcttccggct gcaaaaccag cagatgctgc tccatgtcag ccaacagttc
    16921 gtccgcttca tcaaaaaatg tctgataaaa atcgcttata tccatgctca cgctgtcacc
    16981 tcggttcggc tgatggcatt gtgggaacac tgacctgtgg tgcaacctca ggtttttcca
    17041 gggcgcttac tggctcattc tggctttcgg cgttttcatg caaaatggcc tgttcggctt
    17101 gtttgttcag taccagcagg ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc
    17161 ttaagcgcat cgttgccgcc atgccgacaa cacgtaacac tttgccgcta tccaaccctc
    17221 cgaccatcag ttcgcggcgg gatgcattgg cccgatcggc agaaagctcc cagttgctat
    17281 atcctttctc accgctggcg taggggaaat catcggtatg acctgaaagg ctaatacggt
    17341 tgggaatacc gttcagtaca ggcgcaatgg cgcgcagaat gtcgcgcata tagggttcga
    17401 catcggcact gccggttcta aacatcgggc gattctggct atcgatgatc tgaatacgta
    17461 gaccttcctg gaccagatcg attttgagat ggggacgtaa cgcccgcagt ttcggatcgg
    17521 actctatcaa ctggtcgaga tcaccccgca atttccgcaa tcgactttgc tccatgcgtt
    17581 ttttcagctc ttcgatgttc ggctgcttat tcacttcccc ctggctttgg gtgtaatcat
    17641 caccaccgcc gggaattggg ctttcactat tagaaatgcg atcgccgccc gtaaccgcag
    17701 tcgccagtgg agtccggaag tactccgcaa tctgaatcag ctcttttggg ctggagatgg
    17761 agatcagcca catcaccaga aaaaaggcca tcatcgcagt cataaagtcg gcataagcaa
    17821 tcttccacga tccatgtgct gccccgtggc ttttggcttt gcgtcgtttg acgacaataa
    17881 tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac
    17941 cgcacgcaca tgctcttcca gttcaataaa cgacggacgt tcgctggaat agagcgtttt
    18001 gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc agattagaaa gcagagtgac
    18061 tttgacgcac tgcatcattt tgctggtttc ggcgcttttc tgacgtaaaa cagtcgctaa
    18121 tggggaaata aatccgtaag ccaataaaat gccgaggaaa gtccccacca tcgcatgtgc
    18181 gataagcgca cccagctcgg cggcaggacg atcggctgaa cctaacgcgt gaacgacccc
    18241 cattacagcc gcgacaatac caaacgccgg aagtgagtcc ccgaccagcg ccagactgtt
    18301 cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct tcatccatca gggcttcgat
    18361 ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga taatcgacga taaaatcaag
    18421 catgacgcta tccgcgagga tgcgtgggta gctggcgaag atctcgctct cacggggatt
    18481 ttcaatatca cgttccagcg aaaacatccc catctgccgc gattttgcca tcaaccgata
    18541 aagcagagcc agcagatcca tatacattgc tttggtgtat ttggagcgac gaaacagcaa
    18601 cggcagcgcc ttcagcgtgc ctttaatcgc tttgccatta ttgccgacga taaacgaccc
    18661 aatccctgca ccggcaataa tcaccagttc agcgggttga tagagtgctc caaggcttcc
    18721 accggtcatc aaataaccgc cgaaaactgt accgagaaca accaggtaac ctaataagat
    18781 aagcacgaca tcatccttcc actgttgacc atgacaggat gttcagtcgt caggcgttaa
    18841 cgcgcgattg gggcaaaaaa aagcagcggt acgtcgttac cgctgctgga atgttgcgcc
    18901 tcaccgtatc agttaaacag cctgtactct ctgttcatcc agcagttgtg ggataatatc
    18961 ggcaggattc tgggaaagtt tacgtctttt tactgcccgg gatggcggtt gacataagct
    19021 gcaggcaaag ctgccaacag gctggtgagc gtgggtaata aaattgccgc cgcagcagtt
    19081 gcagctggaa agttgcagta atccactttc aacaaaccgc accaatgtcc aggcacgagt
    19141 taatgccagc agtggtcctt cttctgcttg tgggcactgt tcaaggtata aacggtaggc
    19201 tttgatcacc gcatcgacgc cattacacaa accggttttc agtaaaaact gccatgcatt
    19261 acagaacatc gaagcatgaa cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg
    19321 cagcatgcct ttcggcggtg ggcttccgcg cagttcttta taaagtttta tcaggcgtcc
    19381 gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg tgatcaattc
    19441 cattgccagc tgaatatccc gcgcttcctg aacaatgctt ttttcactca tgatcaggcc
    19501 cttttcttgc gcagcgcttc ttcaggctga ttaacatcat tcagcaagcg tgttgagagc
    19561 atgatcccgg tatgaatttg ctggagatcg tcaacgcggg aatcttgcgt caactgagta
    19621 atcgtctggt ggctgtcaaa acggaagtga caaaccagct gattggtttc tgccagctta
    19681 accatttgcg gaagagtcag tgccgctaat gttgtcgcca tttcttcatt tatgccgaga
    19741 cgaaacatag cggacgcttt gtcctgaaca atcagacgct gtgcaagtag taaatatgac
    19801 aagttgatgt cataaatgtg tttcagcaac tcggagatat gcattattcc cacccagaat
    19861 aaccaacttt atttttatgc ggtctcaccg caccccgtga tgtcgccggg aagccccggt
    19921 aaaaaataat tagcattaga atagttgcga taagctgcaa taagcagaac cacctttttg
    19981 gtttaataag tccttacaaa tagaaagggg tccttacact tatctaagat ttttcctaaa
    20041 tcgacgcaac tgtactcgtc actacacgca catacaacgg aggggggctg cgattttcaa
    20101 taatgtgtga tgcagatcac acaaaa
//
LOCUS       DAFNBJ010000087        19635 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000087 DAFNBJ010000000
VERSION     DAFNBJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.087"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            141..1274
                     /gene="ompN"
                     /locus_tag="L2X49_002966"
     CDS             141..1274
                     /gene="ompN"
                     /locus_tag="L2X49_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HBN4798016.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGSDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1415..1849
                     /gene="uspF"
                     /locus_tag="L2X49_002967"
     CDS             1415..1849
                     /gene="uspF"
                     /locus_tag="L2X49_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBN4798017.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            2025..2960
                     /gene="ttcA"
                     /locus_tag="L2X49_002968"
     CDS             2025..2960
                     /gene="ttcA"
                     /locus_tag="L2X49_002968"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBN4798018.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3089..4462)
                     /gene="dbpA"
                     /locus_tag="L2X49_002969"
     CDS             complement(3089..4462)
                     /gene="dbpA"
                     /locus_tag="L2X49_002969"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBN4798019.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATLGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSILPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGVDIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4940..5923)
                     /gene="zntB"
                     /locus_tag="L2X49_002970"
     CDS             complement(4940..5923)
                     /gene="zntB"
                     /locus_tag="L2X49_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBN4798020.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            6178..7410
                     /gene="dgcM"
                     /locus_tag="L2X49_002971"
     CDS             6178..7410
                     /gene="dgcM"
                     /locus_tag="L2X49_002971"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HBN4798021.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7431..7994)
                     /gene="smrA"
                     /locus_tag="L2X49_002972"
     CDS             complement(7431..7994)
                     /gene="smrA"
                     /locus_tag="L2X49_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBN4798022.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8324..9232)
                     /locus_tag="L2X49_002973"
     CDS             complement(8324..9232)
                     /locus_tag="L2X49_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4798023.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9408..10718
                     /gene="abgA"
                     /locus_tag="L2X49_002974"
     CDS             9408..10718
                     /gene="abgA"
                     /locus_tag="L2X49_002974"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HBN4798024.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10718..12163
                     /gene="abgB"
                     /locus_tag="L2X49_002975"
     CDS             10718..12163
                     /gene="abgB"
                     /locus_tag="L2X49_002975"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HBN4798025.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            12200..13726
                     /gene="abgT"
                     /locus_tag="L2X49_002976"
     CDS             12200..13726
                     /gene="abgT"
                     /locus_tag="L2X49_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HBN4798026.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13737..14252
                     /gene="ogt"
                     /locus_tag="L2X49_002977"
     CDS             13737..14252
                     /gene="ogt"
                     /locus_tag="L2X49_002977"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBN4798027.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            14447..15199
                     /gene="fnr"
                     /locus_tag="L2X49_002978"
     CDS             14447..15199
                     /gene="fnr"
                     /locus_tag="L2X49_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBN4798028.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            15351..16301
                     /gene="uspE"
                     /locus_tag="L2X49_002979"
     CDS             15351..16301
                     /gene="uspE"
                     /locus_tag="L2X49_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBN4798029.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(16351..16608)
                     /locus_tag="L2X49_002980"
     CDS             complement(16351..16608)
                     /locus_tag="L2X49_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HBN4798030.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            16852..17883
                     /gene="ynaI"
                     /locus_tag="L2X49_002981"
     CDS             16852..17883
                     /gene="ynaI"
                     /locus_tag="L2X49_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBN4798031.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(17934..19547)
                     /gene="mppA"
                     /locus_tag="L2X49_002982"
     CDS             complement(17934..19547)
                     /gene="mppA"
                     /locus_tag="L2X49_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBN4798032.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT         4999 a         4856 c         4720 g         5060 t
ORIGIN      
        1 ttcagaggaa ttatttgatt aaggttttac ttaaggcgta acaaatgata tttatcggct
       61 aactgaactt ctcctttagg atgttttcac tccccggaat ggggataaaa ggcaaataaa
      121 ataactaaag gatttattca atgaaaagca aagtactggc acttttaatt cctgccctgc
      181 tcgccgcagg tgctgcacat gcagccgaag tttataataa agacggcaac aaattagatc
      241 tgtatggcaa agttgatggc ctgcattatt tttctgataa ttcagcgaaa gatggcgatc
      301 agagctatgc gcgtctgggt tttaaaggcg aaacccaaat taacgatcaa ctcactggct
      361 acggtcaatg ggaatacaat attcaggcaa acaacactga atcttcaaaa aaccagtcat
      421 ggacccgtct ggcatttgcc gggctgaaat ttgcagatta cggttctttc gattacggac
      481 gtaattatgg cgtaatgtac gacatcgaag gctggaccga tatgctgcct gaatttggcg
      541 gtgactctta taccaatgca gacaacttta tgactggtcg agccaatggc gtcgcgactt
      601 atcgtaatac tgatttcttc ggtctggtaa atggtctgaa cttcgcggtg cagtatcaag
      661 gtaacaacga aggtgccagt aatggtcagg aaggcaccaa caacggacgt gatgttcgcc
      721 atgaaaacgg tgacggctgg ggtctttcca caacatatga tttaggcatg ggctttagcg
      781 ctggtgcggc atacacctct tctgaccgca ccaatgatca ggttaaccat actgcggcgg
      841 gtggtgataa agcagacgcg tggactgctg ggctaaaata cgatgctaac aatatttacc
      901 tggcaacaat gtattcagaa acgcgtaata tgaccccgtt tggcgacagc gattatgctg
      961 ttgcaaacaa aacccagaac tttgaagtca ctgcacagta ccagtttgat tttggtctgc
     1021 gtccggcagt ctctttcctg atgtctaaag gcagtgacct gcacgctgcc ggtggtgcag
     1081 acaacccggc aggtgttgat gataaagatc tggttaaata cgccgatgtt ggcgcgactt
     1141 actatttcaa taaaaacatg tccacttatg ttgactataa aatcaacctg ttggatgaag
     1201 atgacagttt ctacgctgcc aatggcatct ctaccgatga tattgtcgct ttaggtctgg
     1261 tttatcagtt ctaaatcctc ctgcccgctg ttatggcggg ctttttctgc ttattcttcc
     1321 tcctttgatc taaattaaaa atgtgaactc cgtcattaca caaaaagtgt catctggcgt
     1381 tacactttat gcggatacta aaacaggagg ttttatgaac agaacgattc ttgtccctat
     1441 cgatatttcc gattcagaat taactcaacg cgtgattagc cacgttgagg cagaggcaaa
     1501 gattgatgat gcagaggttc atttcctgac ggtaatacct tcactgccct actatgcctc
     1561 tctgggttta gcgtattccg cagaattacc ggcaatggat gacctgaaag cggaagccaa
     1621 atcgcaactg gaagagatca ttaaaaaatt taaactgcca accgacagag tgcatgtcca
     1681 tgttgaggaa ggctcgccca aagaccgcat tctggaaatg gcgaagaaga tccccgctca
     1741 tatgatcatc attgcttccc atcgaccgga tatcaccact tatctgctcg gttccaacgc
     1801 cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt gtgcgctgac actaacgccc
     1861 gcacattgct gcgggctttt tgattcattt cgcaaatgtg ctgacatttt cccctctaat
     1921 ccgtaccata cacgccacag tttttatatc agatttctta tgctgggcgt tccggcatga
     1981 cgcatactct tctgatgcca tataacgaat tgagtcgctt ttaaatgtcg caaaatcaag
     2041 aaattagtaa gaaagaacaa tacaacctga acaaattaca aaaacgtctg cgtcgtaacg
     2101 tgggcgaagc cattgctgac ttcaatatga ttgaagaagg cgatcgcatc atggtttgcc
     2161 tctccggggg taaagacagc tataccatgc tggagattct gcgcaatttg cagcaaagcg
     2221 cgccaatcaa tttttcgctg gtggctgtta acctcgatca aaagcaaccg ggcttcccgg
     2281 aacacgttct gcccgagtat cttgaaaagc tgggcgttga gtacaagatt gttgaagaga
     2341 atacttacgg tatcgtgaaa gagaagattc cagagggcaa aaccacttgc tcactgtgtt
     2401 ctcgccttcg tcgcggtatc ctttatcgta ccgcaacgga actgggggcg acgaagatcg
     2461 cgttgggtca ccatcgtgac gatatcctgc aaacgttgtt cttaaatatg ttctacggcg
     2521 gcaagatgaa aggtatgcct ccgaaactga tgagcgatga tggcaaacat atcgttattc
     2581 gtccgctggc ctactgccgc gagaaagata ttcagcgatt tgccgatgca aaagcgttcc
     2641 cgattattcc gtgcaacctg tgcggttcac agcctaacct gcaacgtcag gtgattgctg
     2701 acatgttgcg tgactgggat aaacgttatc cagggcgtat cgagacgatg ttcagcgcga
     2761 tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct gttcgatttc aaaggcatta
     2821 cccacggttc tgaagtggtt aacgggggtg atctggcgtt tgatcgcgaa gagatcccac
     2881 tacaaccggc gggctggcag ccagaagaag atgaaaatca gttggatgag ttacggctga
     2941 atgtggttga agtgaaataa ccaggatagc gcccgatgcg caagcgtatc gggctactct
     3001 tatggaggcc ggataagacg cggccagcgt cgcatccggc aatcccgcat aagatgttta
     3061 ctcttgcacc cggcaattca acatttcatt attttaataa ccgcacccgg cacgtttttc
     3121 ctttaatctt cccgccctgt aactgtttcc atgctttatg agcaacagcc tgacggaccg
     3181 cgacatagac atgcgccgga tgcacggcga ttttgccaat atctacgcca tcaagcccga
     3241 tgtctcctgt cagcgcacct aatacatcac ccgggcgcat tttggctttt ttcccgccat
     3301 cgatacacaa cgttgccatt tctgcttcca gcggcaaaat ggaactatta gctggcggcg
     3361 tttgccagtt aagttttatc tgcaacatgt cagaaatgat attggcccgc tgtgcttctt
     3421 ccggagcaca gaaactgatc gccagaccgc tatttcctgc acgagctgta cgaccgatgc
     3481 gatgtacatg aacttcaggg tcccacgcca gctcaaagtt caccaccagc tcaagcgatt
     3541 taatatccag accacgcgca gcaacatcag tcgcaaccag tacacgggcg ctaccgttag
     3601 caaaacgtac cagagtctga tcgcgatcgc gttgctccag atcgccgtgt aacgacaatg
     3661 cactttgccc tacttcattc agcgcgtcgc agacagcctg gcaatctttt ttggtattgc
     3721 aaaacaccac acaagaggat ggctgatgca agcttaataa ccgttgcaac agaggaattt
     3781 tgcctttact ggatgtctca taaaattgtt gttcaatagg tggcaaagca tctgttgagt
     3841 caatttcaat cgccaaagga tcgcgttgca ctcgtccgct gattgcggcg atggcttccg
     3901 gccaggttgc cgaaaacaga agcgtctgtc gagatgcagg cgcaaaacgg atgacatcat
     3961 caatggcatc gctaaatccc atatccagca tgcggtcggc ctcatccatc accaaagtat
     4021 tcagcgcatc cagtgatacc gtgccttttt gcaagtgatc cagcaaacga cccagcgttg
     4081 ccacgataat atgcggcgca tgttgcaacg aatcacgctg cataccgaac ggttgaccac
     4141 cgcacaacgt caaaattttg gtattcggca gaaaacgcgc cagccgacgc aattcacctg
     4201 ccacctgatc cgccagttca cgcgtaggac acagcactaa agcctgggtt tgaaatagcg
     4261 acgcatcaat ttgctgtaac aagccgaggc caaaagccgc cgttttgccg ctgccggttt
     4321 tcgcctgcac gcgaacatct tttccggcaa ggatcgccgg aagcgcggcg gcctgcaccg
     4381 gcgtcatggt taaataaccc aactcattaa ggttcgtgag ttgggcggga ggcaaaacat
     4441 tcagggtaga aaaagcggtc acaatctatt ctcgtggtca tcgacgcaaa gttagcaggc
     4501 gcgtatcctc gcagatctac gctcacgatg cgacaattta atcggttctt catcaggtgg
     4561 tgggtcaggc atgggttgcg ggcgagggat cggatcgggc actggaacag gatcgccagg
     4621 aatcggttca gggacaggaa tttgcaaata aataagtgtc gtcatatttc cctccggtca
     4681 ttgggtggac tcttaaaggg tagacgctga taaataacag gcaaaaaaaa gccgactcat
     4741 caaagtcggc gtcgtacgaa tcaattgtgc tatgcagtaa ttcaaaaaag gaagtaagac
     4801 aatatggagc gcaacgccca tcgcttgacg ttgcattcac ctgcaaggga gatattgccc
     4861 tgaatgggta gagagtttat tgacttcgct caaactttgc ggcgtttttg tatacagaca
     4921 gccggaaaaa ttgcttttgt tacaaccatt tactacgatg caaccataaa gcaacaccac
     4981 caataagaac aactaacaga atacaaaaaa ttgaaaatcc gaattgccac ccgccgccag
     5041 ggatcccacc aaggttgacg ccaaataacc ctgtcagaaa ggtactgggt aaaaagacca
     5101 ttgccatcaa tgacattgta taggtacgac gagctaaatt ttcctgcatc acctgagcga
     5161 tttcatccgc catcacgcca gtccgtgcta tacaggcgtc gatttcgtca agaccgcgcc
     5221 caaggcgatc ggcaatatcc tgcatccgac ggcgttggtc atcgctcatc cacggcaaac
     5281 gttcactggc aagacgagca taaacatcac gttgcggtgc catatagcga cgcatcacga
     5341 ttaattgttt gcgcagcaga gccaggaatc cacgcggtgg aatttgctga tcaaggagat
     5401 tatcttcaag gtcgataatt ttatcgtgca gctgctcgat aaattcgctg gaatgatcgg
     5461 tcaacgcatc gcacacatcc accagccacc ctccgcaatc ggtcggaccc gtgccctctt
     5521 ccagatcgct caccacatcg tccagcgcca gcactttgcg ttgtcgggtc gaaacaatta
     5581 accgcccgtc catatataca cgcatggcga ccagttgatc ggggcgttca tcggtgctgc
     5641 cgtttataca gcgcaatgta atcagcgtgc cttcaccgag acggctgact cggggccgag
     5701 tgctctcgcc tgccagcgca tcacgtacgt tattgggaag cagcggtgtt gtcgccagcc
     5761 attgggcgct ttcatggtgt acataattaa ggtggagcca gcagggatgc gcttcatcaa
     5821 tcacatctgt attttccagc ggtttaacgc cgcctctacc atccagcatc caggcaaata
     5881 ctgcatccgg gacattaacg tccgatccct taatcgcttc cacagtgcct ccatcatcaa
     5941 cgcattattt tgtagtctag ccttctggcc ctgttacgca acatctcatc accccattac
     6001 cctgaaatga ttaataaaat tctgtctaaa ttgaatacaa aaagcaaaat gcttttccgt
     6061 atacaaaccg tgtgaagtgt taaatagcgt ctatcattat cagaattatc tgatcatatg
     6121 acgtggcttt tttgcgatcg gatagcaaca aaaattgata aaaataacgg gatctcaatg
     6181 attacgcaca acttcaatac cctggactta ctcaccagtc ctgtctggat tgtttcgccc
     6241 tttgaggaac agttaattta tgccaatagc gcggcgcgac tgttgatgca agacctcacg
     6301 tttagtcagc tacgaaccgg accctattcc gtctcctcac aaaaagaact gccgaaatac
     6361 ctctccgatc tgcaaaacca acacgatatt atcgaaatcc tcactgttca gcgtaaagaa
     6421 gaggaaacag cattaagctg tcggcttgtt ttgcgagagc tgacagaaac agaaccggtg
     6481 attattttcg aaggtatcga agcgccggca acgctgggtt taaaagccag tcgctcggca
     6541 aattatcagc gcaaaaaaca aggtttttat gcgcgctttt ttctgactaa ctctgcacca
     6601 atgttgttga ttgacccctc acgagatggc caaatcgtcg atgctaacct cgccgcgctc
     6661 aatttctatg gttataacca tgaaacgatg tgccagaaac atacctggga aattaatatg
     6721 ctcgggcgtc gcgtcatgcc tatcatgcat gaaatttcgc atttacccgg cggtcataaa
     6781 cctttgaatt ttgttcataa actggcggat ggatctactc gtcatgtgca gacctatgcc
     6841 gggccgattg aaatttatgg cgacaagctc atgttatgta ttgtgcatga tattactgag
     6901 caaaaacggc tggaggagca gctggaacat gctgctcacc atgacgcgat gaccggatta
     6961 ctgaatcggc gacagtttta tcacattacc gagccaggcc aaatgcagca tctcgccatc
     7021 gctcaggatt acagcttgtt gctcatcgac accgatcgtt ttaaacacat taacgatctc
     7081 tatgggcatt ctaaaggtga tgaggtgtta tgcgccctcg cccgcaccct tgaaagttgc
     7141 gctcgcaaag gcgatttggt gtttcgttgg ggaggcgaag agtttgtctt attgctacca
     7201 agaaccccac tggataccgc gctttcgctg gctgaaacta tccgtgtaag cgtggcaaaa
     7261 gtgagtattt cgggcttacc acgctttacc gtcagcattg gtgtggcgca tcacgaagga
     7321 aatgaaagca tcgatgaact gtttaaacgc gttgatgatg ctttgtatcg ggcgaaaaat
     7381 gatggacgca accgcgtgct ggcggcataa gccgcggatg cgtctcgaga tcaacgactg
     7441 cgcttagcgt ggcgctccca gttttcttgc ttcgcctgcg ccgttttacg tagtgcgacg
     7501 taacacgccc cgctgccgcc atgatgcggt agcgcagtgc aatatgcctg aacatcatca
     7561 aattcggtca gccagcgcgc cacatagctg cggacaatat tggcatgcga tttatcatcc
     7621 cgtcctttgc catgaataat cagcacgtta cgcaaaccat cagccatggc ttgttgaatg
     7681 aaactgaaca tcattttgcg gcactcttcc accggctggc gcaaaagatt caggctcgcc
     7741 tgttgcggat atttaccact gcgcagctta tccagcaccc catgttgcaa cccttcccgc
     7801 cgaaactcca gcggctgact tagtgggatg atgtcgagaa atccggtggt gaggaaatta
     7861 tcaagctgca gcgtgtcgat acgctgcggc gcacgttggt tacgcgttgg atgccagtgg
     7921 acatcggtag cacgtttcag cggctggaca tcttccatgg cgtcaagaaa cagcgatttg
     7981 tcgtcaaggt tcatgttaca tcctcccgca attaagagcg cgatatgata accagaccgg
     8041 gtcggtccaa caacgtatta cccaaaattc cagtaataag ttccaaatat tgccgatatt
     8101 ttaagcaaaa tgcttatgca tgattattca ttcacgatat taataatgta acttatattt
     8161 tcgtgaaatc tgtcactgaa gaaaattggc aactaaaggt taaaaccgtt ataacacagt
     8221 caccggcgca gaggagacaa tgccggactt aagacgcgga tgcactgctg tgtgtactgt
     8281 agagtctggc ggatgtcgac agactctatt tttttatgca gttttaactt tgcagatagc
     8341 cgcattctcg ccggaattgc gtgattaatg acgcggtcag tggtgtctgg cgactatcac
     8401 gccgctgaat caaataatag gccgctttcg gtaaaatttc gctaaccggc aacatcacca
     8461 gcccctgtcc gtgcaaggga tcgcagccca tttcttcagg cagtatgctg agaaaatcgc
     8521 tttttgccac cagactgata caggctgaga acgtctcgca gactacaccg acctgtggcg
     8581 tttgcgcctg atcgtcaagc aattcactca actgtttgta gtaactgccg tgtggcgtcg
     8641 gcattgtcca gctgtaatcc agtaactgtt tgatcgaacg ggcaccaatg gcggggtgtc
     8701 ccgggcggca aaagatcgcg aattgctttt ccagtaattt ctcaaaagta aattcgtggt
     8761 cgtacggtcc ctgataatag gtattgatgg tgaaatccaa ttctccctga cgcaattcat
     8821 taatcatcga caccagttgc ccttccataa tgcgtacttt tacctgcgga tgctgctgat
     8881 gaaaacgaga tatgacagct ggcatcagac tgcgggaaat actggccccc atgccgatat
     8941 taatctgccc tgccagttgc ccttgtcgtt ggcgaatttc ctcttgcgct gcgcgcagct
     9001 cttcaagaat aagactggcg tgctgataaa aactttcacc ggcatcagtt aacgtcacgc
     9061 ctttactacg gcgaaaaaag agttgcgccg ctaatccttc ttccagctcc tgaatagatt
     9121 tactcagtgc cggttgcgac atattcaaca ttcggctcgc tccgcgaatg ctgccctgac
     9181 gagccacttc aacaaaagca cgaatttgat gaatttttac ctgaaaagcc atgacgccac
     9241 cgataaccgt tatttatcag accaaagaaa ctggcatcta ctttaatgca gatgattgtg
     9301 tcagggtaat ttatgaacgg ttaaaactgt gaaaaatcag ttagtgataa gtaaaaacta
     9361 tcgctacatg aaccgggtca cactttttac tgatgacggg aaaggttatg gagtctttga
     9421 atcaatttgt taattcgctt gccccaaaat tatcgcactg gcgacgtgat tttcatcact
     9481 atgcagagtc tggctgggtg gaattccgca ctgccaccct tgttgcggaa gaattgcagc
     9541 agctcggcta ttcactggcg ctgggccgcg aagtagttaa tgaaagtagc cggatgggac
     9601 tacctgatga actcactcta caacgcgaat tcgagcgcgc tcgtcaacag ggtgcgctag
     9661 cacaatggat tgcggttttt gaaggtggtt tcactggtat cgtcgccacc ctggataccg
     9721 gtcgccccgg tccggtgatg gctttccgtg tcgatatgga cgcgctggat ctcagtgaag
     9781 agcaggatgt cagccatcgc ccctaccgcg acggttttgc gtcatgtaac gccggaatga
     9841 tgcatgcctg tggtcatgat ggacataccg ccattgggct tgggctggcg cataccctta
     9901 aacaattcga gtccggacta catggcgtca tcaaactgat ttttcagcct gcagaggaag
     9961 gtacgcgtgg cgcgcgggcg atggtcgatg caggtgtcgt agatgatgtt gattatttta
    10021 ctgccgtgca cattggcact ggcgtacctg cgggcaccgt ggtgtgcggc agtgataatt
    10081 ttatggcaac caccaaattt gacgcgcact tcaccgggac cgccgctcac gcaggcgcaa
    10141 aaccagaaga cggtcacaat gccttgttgg cggcagcaca agcaactctt gcactgcatg
    10201 caatcgcccc gcacagcgaa ggagcttcca gagtaaacgt gggcgttatg caggcaggaa
    10261 gcggccgtaa cgttgttcct gcctcggcgt tgctgaaagt ggaaacacgc ggggccagcg
    10321 acgtcattaa tcaatatgtt tttgaccgtg cacaacaagc gattcagggc gcagcaacca
    10381 tgtatggtgt cggcgttgaa actcgtctga tgggtgcagc taccgccagt tctccttcgc
    10441 cgcaatgggt cgcatggttg caaagtcagg cggctcaggt cgcgggggtc aatcaggcca
    10501 ttgaacgtgt tgaagcgcct gcgggttccg aagatgccac attaatgatg gcccgcgtgc
    10561 agcaacatca agggcaagcc tcctacgtgg tgtttggcac acagctggcg gcaggtcatc
    10621 acaacgaaaa attcgatttt gacgagcagg ttctcgctat tgccgtcgaa acgctggcgc
    10681 gcaccgcgct caattttccc tggacgcgag gtatctgatg caggaaatct atcgttttat
    10741 cgacgatgcg attgaagccg atcgccaacg ttataccgat attgccgatc aaatctggga
    10801 tcatccagaa acacgttttg aagagttctg gtcagcggag catctggctt cggcgctgga
    10861 atctgcaagc ttcaccgtta cccgcaacgt aggcaatatc ccaaatgcat ttattgcttc
    10921 gtttggtcaa ggcaaaccgg ttatcgccct gctgggagaa tatgacgccc tggcaggttt
    10981 aagtcagcaa gctggttgcg cgcaacctac atccgcgacg cccggtgaaa atggtcacgg
    11041 ttgcggacac aatttgctgg gaaccgccgc ctttgccgct gcaatagccg tcaagaaatg
    11101 gctggaacaa tatgggcaag gcggcacagt gcgcttttat ggttgtcctg gcgaagaagg
    11161 cggctcgggt aaaacgttca tggtccgcga gggggtattt gatgatgtgg atgcggcact
    11221 cacctggcac ccggaagcct ttgccggcat gttcaatacc cgcacgctgg caaacattca
    11281 ggcatcatgg tgctttaaag ggatcgcagc acatgccgcg aattcccctc atttgggacg
    11341 cagcgccctt gatgccgtaa cgttgatgac cactggcacc aacttcctca acgaacatat
    11401 tattgaaaaa gcgcgcgtac actatgccat cacaaatagc ggcgggatct cgcccaacgt
    11461 ggtccaggcg caggcagaag tgctttatct tatccgcgcc cccgaaatga ccgacgtgca
    11521 gcatatttat gatcgggtcg ccaaaatcgc cgaaggtgcg gcattgatga ccgaaaccac
    11581 ggttgaatgc cgcttcgaca aagcctgttc cagttatctc ccgaatcgca ccttagaaaa
    11641 tgccatgtac catgccctat cccattttgg taccccggaa tggaactccg aagaactggc
    11701 ttttgcgaaa caaattcagg ctacgctcac ccccaacgat cggcaaaaca gtctgaataa
    11761 tatcgccgca accggtggcg aaaacggcaa ggcttttgca ctacgtcatc gtgaaacggt
    11821 actggcgaat gaggtcgctc catatgccgc caccgataac gtgcttgcgg catcgactga
    11881 tgtcggcgac gtcagttgga aactgcctgt tgcccagtgt ttcagcccct gttttgccgt
    11941 cggtacaccg ctacatacgt ggcaactggt tagccagggg cgaacatcta ttgctcataa
    12001 aggaatgctg ctggcggcga aaactatggc agcaaccaca gtcaatctct tcattgattc
    12061 agggctattg caagaatgcc aacaagagca tcagcaagtt acggacacgc aaccgtatca
    12121 ctgccctatc ccgaaaaacg tgacaccgtc acctttaaaa taacaacaac aacgcaaaca
    12181 caacaaccga ggaatgccca tgagtatgtc atccataccg tcgtcctccc aatccgggaa
    12241 gctctatggc tgggtcgaaa gaattggtaa caaggttccc catccttttt tgctctttat
    12301 ctatttgata atcgtactca tggtgacgac ggcaattttg tcagcctttg gcgtcagtgc
    12361 gaaaaacccg accgatggta cgccggtggt ggtgaaaaac ctgctcagtg tagaaggatt
    12421 acactggttt ttacccaatg tgattaaaaa ctttagcggt tttgctccac ttggagcgat
    12481 cctggcgctg gttttaggtg ccggcctagc ggagcgcgtc ggcttactgc cagcgctaat
    12541 ggttaaaatg gcatcgcatg ttaatgcccg ctacgccagt tatatggtgc tgtttattgc
    12601 ttttttcagc cacatttctt ccgatgcggc gttagtgatc atgccaccga tgggtgcgct
    12661 gatttttctg gcggtgggca ggcatccagt tgcaggttta ctggccgcca ttgcaggcgt
    12721 aggttgcggc ttcacggcta atttactgat tgtcacaacc gacgtgttgc tgtcggggat
    12781 cagcacggaa gcggcggctg cgttcaatcc gcaaatgcac gtcagtgtaa ttgataactg
    12841 gtattttatg gccagctccg tagtcgtact gacgattgtt ggcggcctga taaccgacaa
    12901 aatcatcgag ccacggttag gtcaatggca gggaaacagc gatgagaaac tgcagacatt
    12961 gaccgaaagt cagcgttttg gtttacgcat agcaggtgtc gtatcgctac tttttattgc
    13021 tgcgatcgcg ctgatggtga tcccggaaaa cgggatattg cgcgatccga ttaatcacac
    13081 cgtgatgcca tcacccttta ttaaaggtat cgtgccactg atcattcttt ttttctttgt
    13141 tgtctcgctg gcttatggca tcgctacccg cacaattcga cgtcaggcgg atttaccgca
    13201 tttaatgatt gaaccgatga aagagatggc gggatttatc gtgatggttt ttcccctagc
    13261 ccagtttgtc gccatgttta actggagcaa catggggaaa ttcatcgccg tggggctgac
    13321 cgatatactt gaaagttcag ggcttagcgg catcccggcg tttgtcggtc tggcgttgct
    13381 ttcctctttc ttatgcatgt ttattgccag cggttccgca atctggtcga ttctggcccc
    13441 cattttcgta ccaatgttta tgctacttgg ctttcacccg gcatttgcgc aaatcctctt
    13501 tcgtattgcc gactcatccg tattgccttt agcgccggta tctccttttg ttccactgtt
    13561 tcttggattc ctgcaacgct acaaaccaga cgcgaaactg ggtacttact attcgttagt
    13621 cttgccctat ccgcttatct ttttggtggt atggctgctg atgttgctgg cgtggtatct
    13681 tgttggtctg ccgataggtc cggggattta cccacgtttg tcttaagaga gaacggatgc
    13741 tgagattact tgaagaaaaa attgccacgc cactgggtcc actgtgggtg atttgcgatg
    13801 agcaatttcg cctgcgggcg gttgaatggg aagagtacag cgaacgcatg gtgcagctgc
    13861 tggacatcca ttatcgcaaa gaaggctatg agcgcatttc tgccaccaac ccaggcggtt
    13921 taagcgacaa gcttcgtgat tattttgccg gtaatcttag cattattgat acgcttccca
    13981 ctgccacagg ggggacgcca tttcagcgcg aagtctggaa aactctacgc actatcccct
    14041 gcgggcaggt aatgcattac ggccaactgg ctgaacaatt gggccgccct ggcgcggcgc
    14101 gtgccgttgg tgcggcaaac ggatcgaatc ccatcagcat cgtcgtacct tgccatcggg
    14161 ttattggccg aaacggcacc atgaccggat atgcaggcgg agttcagcga aaagagtggt
    14221 tattgcgcca cgaaggttat cttttgctgt aaacattaaa caatttgtgc cagcttgttc
    14281 acacttttat gtaaagttac ccttaacaac ttaagggttt tcaaatagat agacatatat
    14341 ttacatctaa tatcggaatt ctctgctgtt aaggtttgct tagacttact tgctccctaa
    14401 aaagatgtta aaattgacaa atatcaatta cggcttgagc agacctatga tcccggaaaa
    14461 gcgaattata cggcgcattc agtctggcgg ttgtgctatc cattgccagg attgcagcat
    14521 cagccagctt tgcatcccgt tcacactcaa cgaacatgag cttgatcagc ttgataatat
    14581 cattgagcgg aagaagccta ttcagaaagg ccagacgctg tttaaggctg gtgatgaact
    14641 taaatcgctt tatgccatcc gctccggtac gattaaaagt tataccatca ctgagcaagg
    14701 cgacgagcaa atcactggtt tccatttagc tggcgacctg gtgggatttg acgccatcgg
    14761 cagcggccat cacccgagct tcgctcaggc gctggaaacc tcgatggtat gtgaaatccc
    14821 gttcgaaacg ctggacgatt tgtccggtaa aatgccgaat ctgcgtcagc agatgatgcg
    14881 tctgatgagc ggtgaaatca aaggcgatca ggacatgatc ctgctgttgt cgaagaaaaa
    14941 tgccgaggaa cgtctggctg cattcatcta caacctgtcc cgtcgttttg cccaacgcgg
    15001 cttctcccct cgtgaattcc gcctgacgat gactcgtggc gatatcggta actatctggg
    15061 cctgacggta gaaaccatca gccgtctgct gggtcgcttc cagaaaagcg gcatgctggc
    15121 agtcaaaggt aaatacatca ccatcgaaaa taacgatgcg ctggcccagc ttgctggtca
    15181 tacgcgtaac gttgcctgat ttttccgcat aactcactat ccttctgtca tatcattaaa
    15241 tttttctgat ttattgatct ggcagaaggt tcatcactgt ttcattcacc agatatgggt
    15301 taatctttta attacaaact gcgttgacag ttgttgtaag gagaccctgt atggctatgt
    15361 atcagaacat gctcgttgtt atcgatccta accaggacga ccaaccagca ttgcggcgag
    15421 ctgtttattt acatcaacgg attggtggca aaattaaagc ctttttgccg atctatgact
    15481 tctcatacga aatgaccacc ctgctctccc cggacgaacg taccgctatg cgtcagggcg
    15541 tcatcagcca gcgtacagcc tggatccatg agcaggcaaa atattatctc aatgctggcg
    15601 ttcccattga aattaaagtg gtctggcata accgtccctt cgaagccatc attcaggaag
    15661 tgatcagcgg cgggcacgat ttggtgctaa aaatggcgca ccaacatgac cgtctggaag
    15721 cggtgatttt tacgccaacg gactggcatc tgttacgcaa atgcccaagc ccggtgtgga
    15781 tggtgaaaga ccagccgtgg ccggaaggag gcaaggcgct ggtggcggtg aatctcgcca
    15841 gtgaagagcc gtatcataat gcgctcaatg aaaaactggt caaagagacg atcgaactgg
    15901 cagaacaagt caaccatacc gaagttcatc tggttggcgc ttatccggta acgccaatta
    15961 atatcgcgat tgaactgccg gaatttgatc cgagcgttta taacgatgcc attcgtgggc
    16021 aacatttgct ggcaatgaaa gctctgcggc agaaattcgg cattaatgaa aacatgacgc
    16081 acgtagaaaa aggtctgcca gaagaggtga ttcctgattt ggcggagcac ttacaggcgg
    16141 ggattgtggt tctgggcacg gtaggacgca ccggtatttc agcagcattc ctcggcaaca
    16201 cggcggaaca ggtgattgat catcttcgct gcgatctgtt ggtcattaaa cctgaccagt
    16261 atcagacacc cgttgaactg gatgacgaag aagacgatta acgactcttc aataactgac
    16321 aaggcccgag ttatcgggcc tattttttac ttactgctct tcgccgccaa ggaaataaat
    16381 tcccaacggg attgccagca acacagtaaa gaccaggcta taaacaaaaa tcatcgatga
    16441 ctgaatcaca tacattgatg tcgtccactc agacagcgga atattgtact gttctataac
    16501 gccgccgata gtcgcgcgag tcacaaccga cgccgcaata atgaaaaccg ccaacaaacc
    16561 aaagagaaat ttcttccctt tcgctgactt cagtttcgcc ataatcatga tgtaatcctt
    16621 gtgcagacaa atttcttgtt acctaaacgt aacaatacca tgacatttca ccgttgtctg
    16681 ctctgaaaac ctcacctctt agctggattt acaatcaacg cattgattag ttagctaatg
    16741 ttgctacttc cttccctgac tattgatgat ctgaatgaat tgtcgcggta aaatagcccc
    16801 gccaggggtt tccggcatga cgacaattat ctggaggagc ctgataaaac aatgatcgct
    16861 gaactgttta caaataatgc gcttaatctg gtcattattt tcggtagctg cgcagcattg
    16921 attctgatga gcttttggtt tcgccgcgga aatcgtaaaa gaaaaggttt tttattccat
    16981 gcggtgcaat ttttaatcta caccataatt atcagtgctg ttggtagcat tattaattat
    17041 gttatagaaa actataaact caaatttatc accccaggcg ttatcgattt tatctgtacg
    17101 tccctgattg cggttattct gacgattaag ctatttttgc tgattaatca gtttgaaaaa
    17161 cagcagatta aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg cattatcaaa
    17221 atcaccatta ttgtggtgct tgttctgctt tatggcgaac atttcggcat gagcctttct
    17281 ggcttgctga cctttggtgg tattggtggt ctggctgtcg gtatggccgg taaagatatt
    17341 ctgagtaatt tcttttccgg gatcatgctc tatttcgacc gtcctttcag tattggcgac
    17401 tggatccgtt caccggacag aaatatcgaa ggtacagtag cggaaattgg ctggcgaatt
    17461 accaaaatta cgacctttga taatcgtcca ttgtacgtac cgaactcgct gttttcgtcg
    17521 atcagcgtag aaaacccagg acgaatgacc aaccgccgca ttaccacgac cattggttta
    17581 cgttatgagg atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga gatgctgaaa
    17641 aatcacccgg ccatcgacca gcgacaaacc ttactggttt atttcaacca gtttgccgac
    17701 tcgtcattga atattatggt ttattgcttt accaaaacca cggtatgggc tgaatggctt
    17761 gctgcacagc aagacgttta tttgaagatt atcgatattg tacagtcaca cggcgcggac
    17821 tttgccttcc ccagccagac gctgtatatg gataacatta caccgccgga acagggtcgc
    17881 taaatcagac atcactggcg ttatccacaa gggtaacgcc agttcccatc acatcaatgc
    17941 ttcacaatat acatagtccg actgtacgcc acatcttcag ggttattaat gggataacct
    18001 ttcagccacg gtttgattaa tcgtccattg gtatattgat aaattggtgc aatcggtgct
    18061 tgctccatga ggattttttc tgccgcgttg taatcggcat tacgcgcttt aacggtattt
    18121 tccgtcgatg cctgggccag aactttgtca tatgccggat tgttaaagcg tgaaatattt
    18181 cctgaatgcg ttgacgttaa taatgtcagg aaagtggagg gttcattata atcccccacc
    18241 catgaggcgc ggataacatc aaaattgccg gtgttacggc tatcgatata ggttttccat
    18301 tcctgatttt gcaatttaac atctacgcca aggttctttt tccacatcga tgctacagca
    18361 atcgcaattt tttgatggtt ttctgaagtg ttatacaaaa gcgtcagctt cagcggtttt
    18421 tgcggaccat aaccagctgc gctcaacaaa gtttttgcct gcgcattcag ttcttcctga
    18481 ctcatttgtt caaacggcga aggttccggc gtaaatccgg cggtaacatc tggtgtaaaa
    18541 tgccacgctg gcttttcgcc cgtccctaat actttttcgg tcatcaggcg gcgatctatc
    18601 gtcatactta atgccagacg aacgcgctga tctgccgtcg gccctttttg cgtgttaaac
    18661 gcataataat aggtcccgag ctgcggcggc gtataaacct gccccggaat atccttcaac
    18721 agcttctgat acatattttt cgggaaggat tcggtgatat caatatcccc cgcgaggtaa
    18781 cgcttagtgg ctgcggattc ctgattaatt ggcaggaagg tcactttttg cagtaccgtt
    18841 ttggcgttat cccaataatg ggtattcggt accacgacca gtttttcatt gactacgcgc
    18901 tctttaagaa cataagcgcc attgccaatc agatttccgg gtttcgtcca ctctttaccg
    18961 ctttctacgt tggctttttg caccgggaag aaggcaaagt tagcggttaa attcacaaac
    19021 cacggcaacg gtttatcaag ctgaattttc aaagtatggg catcaactgc ggtgacgcca
    19081 agctggtcag gcgtagcttt accatcaata atcgcctgtg cgttgttgat tcccgccagc
    19141 gcggcaaacc atgcaaatgg cgatagagtt ttcgggtcca ccagacgttg ccagctgtag
    19201 acaaaatctt gcgccgttac cggtgtgcca tccgcccatt ttgcgttatc gcgcagggta
    19261 aaagtccaga tacggttgtc attacttttc cactgagtcg caacgccggg gacaatctcc
    19321 cctttctcgt tctgattcac cagtccttca aacagatcgc gaatgacctg aatctctggc
    19381 aggcccacgg ctttagcggg atccagcgac gcaggctcat ctttaatgtg gcgcaccagc
    19441 tcctgcttct ctgccagtac tgtgccgctc ggaacttctg cagcatacga aagagaaatg
    19501 ctgctgacca acagcgcaca acacgtgact gaaacagagt gcttcataag tttccctcta
    19561 accgacatta aaattgatgc gtaattattt gtttcgcctt aaagaaatgc aaacaacttc
    19621 catcaaaaaa gtgac
//
LOCUS       DAFNBJ010000088        19545 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000088 DAFNBJ010000000
VERSION     DAFNBJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.088"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            208..1305
                     /gene="mltA"
                     /locus_tag="L2X49_002983"
     CDS             208..1305
                     /gene="mltA"
                     /locus_tag="L2X49_002983"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBN4798033.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1544..2350
                     /gene="tcdA"
                     /locus_tag="L2X49_002984"
     CDS             1544..2350
                     /gene="tcdA"
                     /locus_tag="L2X49_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBN4798034.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAKVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2401..2844)
                     /gene="csdE"
                     /locus_tag="L2X49_002985"
     CDS             complement(2401..2844)
                     /gene="csdE"
                     /locus_tag="L2X49_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBN4798035.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2844..4049)
                     /gene="csdA"
                     /locus_tag="L2X49_002986"
     CDS             complement(2844..4049)
                     /gene="csdA"
                     /locus_tag="L2X49_002986"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBN4798036.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4241..4468
                     /locus_tag="L2X49_002987"
     CDS             4241..4468
                     /locus_tag="L2X49_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBN4798037.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4819..5736
                     /gene="gcvA"
                     /locus_tag="L2X49_002988"
     CDS             4819..5736
                     /gene="gcvA"
                     /locus_tag="L2X49_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBN4798038.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5755..6150
                     /locus_tag="L2X49_002989"
     CDS             5755..6150
                     /locus_tag="L2X49_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBN4798039.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6143..7243
                     /gene="rlmM"
                     /locus_tag="L2X49_002990"
     CDS             6143..7243
                     /gene="rlmM"
                     /locus_tag="L2X49_002990"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBN4798040.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7287..8018)
                     /gene="fucR"
                     /locus_tag="L2X49_002991"
     CDS             complement(7287..8018)
                     /gene="fucR"
                     /locus_tag="L2X49_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBN4798041.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8076..8498)
                     /gene="fucU"
                     /locus_tag="L2X49_002992"
     CDS             complement(8076..8498)
                     /gene="fucU"
                     /locus_tag="L2X49_002992"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBN4798042.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8500..9918)
                     /gene="fucK"
                     /locus_tag="L2X49_002993"
     CDS             complement(8500..9918)
                     /gene="fucK"
                     /locus_tag="L2X49_002993"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HBN4798043.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQLLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10027..11802)
                     /gene="fucI"
                     /locus_tag="L2X49_002994"
     CDS             complement(10027..11802)
                     /gene="fucI"
                     /locus_tag="L2X49_002994"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBN4798044.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQKEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11835..13151)
                     /gene="fucP"
                     /locus_tag="L2X49_002995"
     CDS             complement(11835..13151)
                     /gene="fucP"
                     /locus_tag="L2X49_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBN4798045.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13698..14345
                     /gene="fucA"
                     /locus_tag="L2X49_002996"
     CDS             13698..14345
                     /gene="fucA"
                     /locus_tag="L2X49_002996"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBN4798046.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14373..15521
                     /gene="fucO"
                     /locus_tag="L2X49_002997"
     CDS             14373..15521
                     /gene="fucO"
                     /locus_tag="L2X49_002997"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBN4798047.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15576..16331)
                     /gene="xni"
                     /locus_tag="L2X49_002998"
     CDS             complement(15576..16331)
                     /gene="xni"
                     /locus_tag="L2X49_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBN4798048.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVVGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16443..17810)
                     /gene="sdaB"
                     /locus_tag="L2X49_002999"
     CDS             complement(16443..17810)
                     /gene="sdaB"
                     /locus_tag="L2X49_002999"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBN4798049.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVIYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17868..19157)
                     /gene="sdaC"
                     /locus_tag="L2X49_003000"
     CDS             complement(17868..19157)
                     /gene="sdaC"
                     /locus_tag="L2X49_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBN4798050.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4804 a         4824 c         5162 g         4755 t
ORIGIN      
        1 ccgtggatgc gcactatact ccgctcgcgt tttcatgcaa cctttttttt accccgatca
       61 cggatttggc atgattttga acaaaaacaa accactaaag cctatttttt gtgcaaaatt
      121 tgcgtcaggg agaggctgct catttcatta aacgcggcgg tttgttatct tcgttgcgcc
      181 ttatttttta acctgaagaa gagaacaatg aaaggacgtt gggtaaagta ccttcttatg
      241 ggcacggttg tggcaatgct tgccgcctgc tcttccaaac caaccgatcg cggacagcaa
      301 tataaagacg ggaaatttac ccagcctttc tctctggtga accagccaga tgccgttggc
      361 gcgccgatta acgccggtga ttttgccgag caaattaacc atatccgtaa ttcgtcaccg
      421 cgtctgtatg gcaaccagag taatgtttat aacgcggtgc aagagtggct gcgcgcaggc
      481 ggtgataccc gcaatatgcg tcagttcggc attgatgcct ggcagatgga aggtgccgac
      541 aactatggta acgtgcagtt tacaggctat tacacgccgg taattcaggc gcgccatacc
      601 cgccagggcg agttccagta tccgatttac cgtatgccgc caaaacgtgg tcgtctgccg
      661 tctcgtgcgg agatctacgc aggggcgctg agtgataaat atattctcgc ttacagcaac
      721 tccctgatgg ataacttcat tatggatgtg cagggtagcg ggtatatcga ctttggtgat
      781 ggcagtccgc ttaacttttt cagctatgca gggaaaaacg gtcatgccta tcgcagcatt
      841 ggtaaggtgc tgatcgaccg tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt
      901 cgtcattggg gcgaaacaca cagtgaagcc gaggttcgcg agctgctgga acagaacccg
      961 tctttcgtct tctttaaacc gcaatctttt gcaccggtga aaggggcaag tgcggtgccg
     1021 ctggttggcc gtgcttctgt tgcctccgat cgttcaatta ttccgccagg tactaccttg
     1081 ctggcagaag tgccgctgct ggataataac ggcaaattta acggtcagta cgaattgcgt
     1141 ctgatggtgg cgttggatgt cgggggcgca atcaaaggcc aacacttcga tatctatcaa
     1201 gggatcgggc cggaagccgg acaccgcgca ggttggtaca accactatgg acgtgtctgg
     1261 gtgctgaaaa ccgccccggg cgcaggtaac gtctttagcg gctgatgtga tattctgtat
     1321 gccgttttta cggatatgac agggtgaggg gtaacctcac atcctgatta ttgactaagt
     1381 gcgcgttgtt catgccggat gcggcgtgaa cgccttatcc ggcccacaaa tcatgcaaat
     1441 ccaaaatatt gcaggagtta tgtaggcctg ataagcgtag cgcatcaggc atttatgcgt
     1501 ttagggtgag ggaaaacctc acccttttta attctgaggt gctatgtctg tggtaattag
     1561 tgatgcatgg cgtcagcgtt ttggtggcac agcgcgtctg tatggtgaaa aagcgttgca
     1621 actgtttgct gacgcgcata tttgtgtggt cggtatcggt ggtgtcggtt cctgggcggc
     1681 ggaagcgctg gcgcgcacgg ggattggcgc aatcacgctt atcgatatgg atgatgtgtg
     1741 cgtcaccaat accaatcggc aaattcatgc cctgcgcgat aacgttgggc tggcaaaagc
     1801 gaaagtcatg gcggaacgta ttcgccagat taacccggag tgccgtgtaa cggtggtgga
     1861 tgatttcgtg acgccggata acgtagcgca gtatatgagt gccggttatt cgtacgtgat
     1921 tgatgccatt gatagtgtac gacccaaagc ggcgctgatt gcttattgtc ggcgcaataa
     1981 aatcccgctg gtcacgaccg gtggcgcggg tgggcagatt gacccgacgc agattcaggt
     2041 taccgatctg gcgaaaacga ttcaggaccc gctggcggcg aagttgcgcg agcgcctgaa
     2101 aagcgatttt ggcgtagtga aaaacagtaa aggtaagctc ggcgtggatt gcgtgttttc
     2161 tactgaagcg ctggtgtacc cgcagtcaga cggtacggtg tgtgcgatga aagccacggc
     2221 agaagggccg aagcggatgg attgtgcatc tggatttggc gcggcaacga tggtgaccgc
     2281 cacctttggg tttgttgcgg tttctcatgc gctgaagaag atgatggcga aagcggcgcg
     2341 tcagggttaa acaatcaggt cggatgagac gcgaacgtct tatccgaccc ggttctgtgc
     2401 ttaaacctgc ttcgtcgcag cgataatcgc ctcgcttaac gcattcaacc cctggctgcg
     2461 tgaggcgcta agctgcccac gcaatcccag ctcatcaaac aatgccagcg gcgactgtgc
     2521 ctgcaattcg gcggcggttt tcccctcaac ggcagtcaac agcaccgcca gcaggccgcg
     2581 cactatacgt ccttcgctgt cgccaaagaa gtgcatttta ccgttttcag ccacggtata
     2641 tcccagccag acgcggtttt cgcatccggc aatctcttta gcctgcgctt ttaactcgtc
     2701 tggcaaagcc ggaagctgtt tccccagcat gatcaactgg cgatatttat cttcccattg
     2761 cgacaacggt gcgaaggtat tgcgtaacgt ttctgcggtt acggttgtgc cgaacggatg
     2821 tccggcgaat tgcgggtttg tcattaatcc accaataatt ccagcgcgcg gtcaacggca
     2881 ttcaccagcg catccacatc actctttgta ttatatggcg caaaagaggc gcgcagtgtg
     2941 ccggttacgc ctaattctgc cagtagcggc tgagcgcaat gctgcccggc ccgcagggca
     3001 ataccgtact ccgccagcag cgtcaccata tcgctatgat gaacgccagc aaaatcaaag
     3061 gccagcaggc tggaatcctg gcagcggaat gaacgaaagc cgggacgttt cgccagcgca
     3121 tcttccgcca gcgttgctaa gctacggctc cagctttcgg cctggttgat atcgtaatct
     3181 gccagccatt ccagcgccgc gcttaatcct atgacaccag cgacatttgg cgttccagct
     3241 tccagtttcc acggcgcaga ttgagtcgtg aagccgtcaa aactcacttc gtgaatcatt
     3301 ttgccgccac ccagccaggg cgacatcgct tccagcagtt ctgatttgcc atacagcacg
     3361 ccgatacccg tcgggccata cagtttgtga ccagagaaag catagaaatc gatatctagt
     3421 tgctgaacat ctgcggggaa atgcaccgcc ccctgagcac catcaaccat caccaccatc
     3481 ccggctgaat gagcaaaggt aatcgctcgc gccagatccg ggcaaccgcc agtaacgttc
     3541 gacatctgac ccaacgccag aatccgacta cggggagtaa tcagttctgg caacaaatcg
     3601 acatccggca gtcgctgcgc attaagcggc aatttcacca ctttggctcc agtttgttgg
     3661 gcgaccatca gccaggggac gaggttggcg tggtgttctg ccacgctgac aataatctca
     3721 tcgcccagtt gcagacgcgg acgcgcatag cattgtgcaa ccatgttgat ggattcagtg
     3781 gtgccgcgcg tccagacgat agttttatca tccggtgcat tcagtaattg cgccactttc
     3841 tctcgtgcag cttcataacg cgcggtcagg cgttgggctt cggcaaactg gctgcgatgg
     3901 acgtttccgg cgctcaggct gtaaaactgt cgggtggctt caaccacggc ttcaggttta
     3961 agcgcggtcg cggcgctgtc gagatagacg cccgcatcct gtagagcggg aaactgggcg
     4021 cgaaactgcg cgggattaaa aacgttcatg gtactcctcg gcttgatgca ccgatcgtga
     4081 cgcaaaattc actttgatac aaggagagtg ctaaccatca gaattatctg gatatcctgg
     4141 cgaagccgcg taagtaacat atgctgaata gtgttaggcg ttaattttag cctgttaatc
     4201 aatactatta agatttatta atcgctttaa ggagaagatg atgaaaaaga ctgccgcaat
     4261 tatttctgcc tgtatgctga cttttgccct gagcgcctgt tccggttcga actatgtgat
     4321 gcacaccaat gacggacgta ccatcgtctc tgacggcaaa ccacagactg ataacgatac
     4381 cggtatgatt tcgtataaag acgctaatgg caacaaacag cagatcaacc gtactgacgt
     4441 gaaagagatg gtcgaactgg atcagtaatt cgcgatcgca aggtaaaaaa aagcaccgca
     4501 attaggcggt gctacattaa tcactatgga cagacagggt aaatgtacag gaagtgaaaa
     4561 aaggtagctt tgctaccatg gtctgaatcg cagaccaatt gcaaacacaa caacacaaca
     4621 tcacaaccgt aagccaaaag ttcaccagaa cacgcattcc gataaaactt ttcgttccgg
     4681 ctcaggaagt gccgccacta taggtatttg ctggtagaag ctcaacggac aatttataat
     4741 ggctcagatt aaaaaaacta ataggttaca cagtgtgatc taattgttaa attcatttaa
     4801 catcaaagtt taaaagccat gtctaaacga ttaccaccgc taaatgcctt acgagttttt
     4861 gatgccgcag cacgccattt aagtttcact cgcgcagcag aagagctttt tgtgacccag
     4921 gccgcagtaa gtcatcaaat caagtctctt gaggattttc tggggctaaa actgttccgc
     4981 cgccgtaatc gttcactcct gctgaccgag gaaggtcaaa gctatttcct cgatatcaaa
     5041 gagatatttt cgcaattaac cgaagcgacg cgtaaactcc aggcccgtag cgccaagggg
     5101 gcgttgacgg tcagtttact ccccagtttc gccattcatt ggttggttcc gcgactttcc
     5161 agctttaatt cagcttatcc gggaattgac gttcgaatcc aggcggttga tcgtcaggaa
     5221 gataagctgg cggatgatgt tgatgtggcg atattttatg gtcggggcaa ctggccgggg
     5281 ctacgggtgg aaaaacttta cgccgaatat ttattgccgg tgtgttcgcc gctactgctg
     5341 actggcgaaa aacccttgaa gaccccggaa gatctggcta aacatacgtt attacatgat
     5401 gcttcgcgcc gtgactggca gacatatacc cgacaattgg ggttaaatca tatcaacgtt
     5461 cagcaagggc caatttttag ccatagcgcc atggtgctgc aagcggctat tcacgggcag
     5521 ggagtggcgc tggcaaataa cgtgatggcg caatctgaaa ttgaagccgg acgtcttgtt
     5581 tgcccgttta atgatgttct ggtcagtaaa aacgcttttt atctggtttg tcatgacagc
     5641 caggcagaac tgggtaaaat agccgccttt cgccaatgga tcctggcgaa agccgctgct
     5701 gaacaagaaa aattccgctt tcgttatgaa caataattta cgtagggtac gaccatgacc
     5761 agccgtttta tgctgatttt cgccgccatt agcggcttca tttttgtggc tctgggcgct
     5821 tttggcgcgc atgtgttaag taaaaccatg ggcgccgttg agatgggctg gatccagacc
     5881 ggcctcgaat accaggcgtt tcatacgctg gcgatcttag gtctggcggt ggcaatgcag
     5941 cgtcgcatca gtatctggtt ttactggagt agcgttttcc tcgcgttagg cacggtgttg
     6001 ttcagcggca gcctttattg cctggcgctg tcccatctgc gtttgtgggc gtttgtcact
     6061 ccggttggcg gcgtgagctt cctcgcgggc tgggcgttaa tgttagttgg tgctatccgt
     6121 ttaaagcgca agggcgtaag tcatgaataa ggttgtattg ctgtgccgtc cgggctttga
     6181 aaaagagtgc gccgcagaaa ttaccgataa agccggccag cgggaaattt tcggttttgc
     6241 ccgcgtgaaa gagaatgcgg gttatgtcat ttatgaatgt tatcaacctg atgatggcga
     6301 taagttaatc cgtgagctgc cgttcagttc attaattttt gcccgccagt ggtttgtggt
     6361 gggggaactc ctgcagcatt tgccgccaga agatcgtatt acccccattg tcggcatgtt
     6421 acagggcgta gtagagaagg gcggtgaact gcgtgttgaa gttgccgata ccaacgaaag
     6481 caaagagtta ctgaaattct gccgtaaatt tactgttccg ctacgcgctg ccttgcgcga
     6541 tgccggggtg ctggcgaact atgaaacgcc gaagcgtccg gttgtgcatg tattctttat
     6601 tgcaccaggc tgctgctata ccggttactc atacagcaac aataattcgc cgttctatat
     6661 gggcattccg cgcctgaaat ttccggcaga tgcgccgagt cgttccacgc tcaaactgga
     6721 agaggcattt catgtgttta ttcctgcgga tgagtgggat gaacgcctgg cgaacgggat
     6781 gtgggcggtg gatttaggcg cttgccctgg cggctggacc taccaactgg tgaagcgcaa
     6841 catgtgggtt tattccgtcg acaacggccc gatggcgcaa agtctgatgg ataccggaca
     6901 ggtgacgtgg ctgcgggaag acggtttcaa attccgtccg acgcgcagca atatctcctg
     6961 gatggtatgc gatatggttg aaaaaccggc gaaagttgcg gcattgatgg cgcagtggct
     7021 ggttaatggc tggtgccgtg aaaccatttt caacctcaaa ctgccgatga aaaaacgcta
     7081 cgaagaagtg tcacacaatc tggcgtatat tcaggcacag cttgatgaac atggcataaa
     7141 tgctcagatt caggcgcggc agttgtatca cgatcgcgaa gaagtgacgg tgcacgtccg
     7201 ccgcatctgg gctgcggtgg gtggtcgtcg cgacgagcga taacaggcaa ggccggggcg
     7261 tcgtcttcgg cctgctaccc tttccgtcag gctgttacca aagaagttgc aacctggcgc
     7321 tcatcagaaa taatgtgcgt tacctcatcc agatgcccga tgcgggcttc ccctgaacga
     7381 ttaaatttac ttttatcaat caataacaac gattgcgcgg cacgttttaa tagcatcgat
     7441 ttgtaatcag cgttgatggc attggagtcc cacagtgcgc cgctgctatc gatcccttca
     7501 caagaaaaaa taaacagatc gatttccagc gatttaagtt gggaaatcag cgagggattg
     7561 acgtaacagc catatttgcg ctcaagtgtg ccgccggaac tgatcagttg aatgcgttcg
     7621 cgtttaccga gttcatggca aatcggatgg ctattggtga agacctgaat gttgatgtca
     7681 ggcaactggc gtgccagata ccagcaagtt gaactggcgt ctaaggctat caccatccct
     7741 tcttcaatcc acgcgagcgc ctcgcgcgcg atatctgctt tatgcgcata atggcttttc
     7801 agccggatgt gaaagggatc gccactgtct tgattttgac ggtggatata tttagcgcgt
     7861 ccatgattgc gcagaatttt accctgcgtc tgtaattcat tgagatcgcg acgaatggtt
     7921 tctttactga cctttagctg ttcagagaga gcttccgtgg tcaggctggt atggttcagc
     7981 agcaggtcga ctatcgcttg ctggcgtgcc gctttcatat ttttcacccc atcattcgat
     8041 aaccgcccgc gtcatgcggg cgtaccggta tgagattacg gtgttacccc tttttttaaa
     8101 agaatattcc cgtacttcgc tcgttcgcct gtgataacga tcgcaaaggc tttttgcgcc
     8161 cgttcataaa acgcaaaacg attgatgcgg atgatgtcag gacacggggc ttgtagtgaa
     8221 agcgcattac ggtaacgtcg ttctacttca ggatcgagag tgtcaccttc taccgccgcc
     8281 atcatcacca gcggcggtgc ataactgtcc agttcaaata acgggataat cgcctggagc
     8341 aagtcgctca ccaacaggcc atcagcgcgg atcacctgcg gtcccatcga atgggcggga
     8401 aagtgagcat cggaaaaaat aatttcatct ccatgtccca tctctgccag cactttcaat
     8461 agttcgggag aaattaacgg cgaaattgtt ttcagcattt cacacttcct ctataaattc
     8521 aggttcagtt tgcgggtaga aataacggta ctgataatga atctgtgcgc gggcttcttc
     8581 cgggctgtta aattccccta cgccatacca accgaacagc gcagctcctg cgacggtcgt
     8641 ttcggcgtcg tcgagaactt ttaccggaat atcaagcata ttggctttaa tctgattcca
     8701 caatgtgttg cgacttcctc caccgactaa caataattca gaggccttaa agtgcccgat
     8761 tttttccagc aactgtagat tgcgctgtaa ttgcgcagtt aacccttcca gcgccgcgcg
     8821 atagaaatgc ccccgcgtgg tattaagcgt cactccttgc cagccagcgt tctgacacga
     8881 caataaatca cactgcattt ttacgccatc cgcgccaggc gcgatcagac gagcttcttc
     8941 aatcaacatt tgccagggtg tttcagccgt ccagaacagt tttctcaccc attccagcac
     9001 gccggatgcc agccattgca tacctgggtt atacaaccct gcctggctat ccagttcgca
     9061 ggtggaaccg gcgtactgac ttaacagcga agtatcaacc tgggcgctac ggaccattaa
     9121 aatttcccat gtaccggaag agagcacggg ttcattttgg tcggcaccag cgccaaaaag
     9181 ggcgaactgg gtatcgtgac ctgcggaaat caccggtatg ccaacgggta agccgagcat
     9241 tgctgcggcg ctgttctgta gcgtaccaat ctgttcaccc gcttccacca gacgagggaa
     9301 gagtcggcgt ggaataccgg tggcttgtaa aatttgcgga ctgaaatcgc gttgctggat
     9361 atccagcatc tggctggttc cggccatcgt gatatcagta gtgaattcgc cggttaaacg
     9421 gtggttaatc agcgacgaaa taaagagcca ggcgtgcgcg cgttccagca gttgtggatg
     9481 attttctttc aaccacacca acttatataa cgtattgaaa ctaaaggctc cgacgccaga
     9541 aatagcctgc aaccgctgtg cggagatcaa ctgttcaata ttgtccataa ccgctgctgt
     9601 tcgcggacat ttccagctaa tgatcggata gagcagattg ccttgcttat ctaccagagc
     9661 gccatccaca ccaaaggtgg tgacggcgat accgcggatg tggcattcag tcagttcact
     9721 attgatttgc cgacagcaat cagcaaagcg ttgcaaaatg gcgtctaaag accactggtg
     9781 ccaggtgttg ttttccatcg cgatatcgct ggcattaggc gttgaggcgc gggcaacaat
     9841 ttttccctgc cgattaaccg cgatggccct gacattggtc gcgccacagt cgagtaccag
     9901 gataacttct tgtttcataa tcgctcctgc aatatagccg gataacattg cttatccggc
     9961 taaccactct tgcttgttca ggcccggtag ccggagcgac cgggcatcac atcagggagt
    10021 aatgtattaa cgcttgtaca acgggccgta gttctggcaa gcgcggtaat cctgaccttc
    10081 aatatccatg ccgtgcgcgg cccaggcaga aggacgatat actttggtct cttcaacgtt
    10141 gtgcatacat accgggatac gcagcatgga ggcgagagtg ataaagtctg cgccaacgtg
    10201 gccgatggtc agaaccccgt ggttagcgcc ccagttcgcc attaccgaat acacatccgt
    10261 aaacggccct ttaccggtga ggcgcggtgc aaaccaggtg gttggccagg ttgagttggt
    10321 gcgtttgttg aggatgtcat gcacatcctt cggcaattcc acgctccagc cttccgcgat
    10381 ttgcagtacc ggtcccaggc ctttgatgat gttgacacgg gtcatggtga acgggacgcc
    10441 gccttcggta aggaagcggg aagagtaacc gccgccacgg aagtattcgt ggatcgccgg
    10501 gcaccattcg gtagcggcga ggcaagcgtc agcctctttc tgagagattt cccagtgtgg
    10561 cttcatcgtc gggttacctt cgctgtcgcg ttgtttacag gaaccgtcca gcgcagcaga
    10621 accggagttg atcaaatgga tgatgccgtg ttctgccagt ccatcgagtt tatgccccgt
    10681 tacacgctca attgcttctg gtgaccagta ggtacgcaca tcggcaaata cctgagcggt
    10741 gccggtgagc tggtgaccca ttagcattgc cacgccgtta agactgtcgt tttcggtcgc
    10801 cacgacaaag ggttcgcgca cgccattcca gtcaaatgaa ctgttaagga tcgcttcggc
    10861 ggtgtcacca ttgggatatt gatcggtcca gtgacgttgc ccctggaagc ccgcagcgat
    10921 ggcgttgtag ccaagtgatt cttccacgcg accaatatcg gccagtttgc tgttgccttg
    10981 catcatgtcg cggatacaca tcgccatcag taaactttcg cgcagaactg cgcggctttg
    11041 ctcggcattg cgttgatact gtttgttatt ttcatcttcg ccatagcgga agtttttatc
    11101 agcccaggcc agtgccattt ccagttcggc ttcgtcgtaa atcttctgat cgatacggcg
    11161 acgcagttcg gtcatatcca ccgcctggac tttcattccc agccaggatt caaagaagtt
    11221 gtgatcaaca atggaaccgg cgatacccat cgaaacgccg cccagcgaca gatagctttt
    11281 acctttcatg ctggcgacgg ccaaaccggc gcgggcaaag cgcagcagtt tttcttcaac
    11341 atcggcagga atcgatgtgt catcggcatc ctgaacgtca tgaccgtaaa tggagaatgc
    11401 cgggatgcct ttctggctgt gagctgccag agccgctgcc aggtaaacag cgccgggacg
    11461 ttcagtgccg ttaaagcccc aaatggcttt cgggcgggtt ggatccatgt cgatggtttc
    11521 actgccatag caccagcaag gcgttaccgt aatggtgagg cctacattct gactgctgaa
    11581 tttttcttcg caagcagcgg cttcagccat acccgcgata caggtatcgg aaatgacaca
    11641 ctcgacggca gctccgcagg catggcgcag tttctcggtc agcagtgcgg ccgtagcttt
    11701 cgccatattc attgtttgtt cttcaagcga ctcacgaaca cccatgcgac gaccgtcaat
    11761 aaccgggcgg ataccaattt tcggtaagct gatttttttc attacagatt cctcacttta
    11821 ttcggaaaat atgttcagtt agttgccgtt tgagaacgga aacgggcaaa gataaagatg
    11881 accgcgaagc agagtgcggg gatcagttca gcagtgggga tgttgcccgc cgcgtcactg
    11941 acaaaaccca tgaccggagt gacaataccg ccgccaatga tggtcataac gatgaaggac
    12001 gaaccgtatt tggtgtcctg gccgagattc ttaatgccca gcgagaagat tgttgggtac
    12061 tgaatcgaca taaaggcgct gcataaagtc agggctatta agcccacatg accgccagcg
    12121 aaggctgaga tcaggcacag tgccatagcg attaatgcgt aggcggccag gactttgtgt
    12181 ggtgcgaagc gactgatgag ccaggtaccg gtgaaacgac caataaagaa gcacaccatg
    12241 gttccggtta aatagttagc ggcaaagccg gcagtcatac ctggaatttc ttctacagcg
    12301 tagcgaatca aatagctcca gcaggccgtt tgtgcgccga catagcagaa ttgcgctaat
    12361 accgcccagc gccagtgacg aatacgcgcc agacgagaaa gcgatgcgga gaacgatcct
    12421 tgtttggcgt cactgtgatt atcactctgc aatgccggga atttcgtcag catgatcagc
    12481 agggcgacca gtaacacgat agccacgatg atcatataag gtgtctgtac cgataatacc
    12541 aggctgtgtt tatacgcact caattgctct ggagacattt tatcgagaac gtcttgcgat
    12601 tgatgtggca cgttagacaa aataagactt tgcccaaaga caaccgcgat aattgcgcca
    12661 aacgagttaa atgtttgcgc aagatttaag cggaagtgac cactgctttc tggccctaat
    12721 accgtaacaa aagggtttgc ggcagtttcc agacaaccta atccggctgc aataataaat
    12781 aggccaacta aaaataaggt gtagttcatt atttctgcag cgggccagaa taatgcagcg
    12841 cccaaggcat ataaaaataa tccggtaata atccctgctt tataactgag ttttttcatc
    12901 aatatcccag cagggattgg gataatgaaa taaccaaagt aaaaggccga ttggatcagg
    12961 ccagcctgga aatttgtcag cgtaaaagcc tgctggaatt gaggtaataa aatgtcgtta
    13021 aggttattgg ctaccgccca aagaaaaaac agtgagcaca gcagcgcgaa tggaataatg
    13081 taacttctgc tttgccctgc atctttatct accgcacggt aactctgcgt ttgtattgat
    13141 gtgtttccca tagcatcctc ttaggttcag aagcttaagc acgtaatcgg ttatgttttc
    13201 aaaagtgcaa aaccagcgag ttcgggcttc atatcagtga atatttgcct gtctgaaatg
    13261 aactcatgga ttcattttca aataaaaaac gctctgtaat atgacggcgg tcacacttat
    13321 tagctggaag gtgactttat gtgactacca tcactttaat aatcgaaatt tcatgcccgt
    13381 ttcagaaaat aaatcataaa aaacacaaac cagatgaccg tattaattgt ttatgccgaa
    13441 aacgggcatt cctggcttgg gtgaatagaa aaatgaccgt tttgataaat atttttaaga
    13501 gcggtcattt ttaatggggt tataaaaaga gaacttgcag ttatttactg tgattagttg
    13561 aaccaggtca caaaatataa gcttgtgaat ttctttcttg ttaatcagga agaagatggt
    13621 gggtaattaa acggctaatt caatagtgtg aaaggaacaa cattattgcc ctgttttgaa
    13681 tcagagagag gtagctaatg gaacgaaata aacttgctcg tcagattatt gacacttgcc
    13741 tggaaatgac ccgcctggga ctgaaccagg ggacagcggg gaacgtcagt gtacgttatc
    13801 aggatgggat gctgattacg cctacaggca ttccatatga aaaactgacg gagtcgcata
    13861 ttgtctttat tgatggcaac ggtaaacatg aggaaggaaa gctcccctca agcgaatggc
    13921 gtttccatat ggcggcctat caaagcagac cggatgccaa cgcggttgtt cacaatcatg
    13981 ccgttcattg cacagcagtt tccattctta accgaccgat ccccgctatt cactacatga
    14041 ttgcggcggc aggtggtaat tctattcctt gcgcgcctta tgcgaccttt ggaacacgcg
    14101 aactttctga acatgttgcg ctggctctca aaaatcgtaa ggcaactttg ttacaacatc
    14161 atgggcttat cgcttgtgag gtgaatctgg aaaaagcgtt atggctggcg catgaagttg
    14221 aagtgctggc gcaactttac ctgacgaccc tggcgattac ggacccggtg ccagtgctga
    14281 gcgatgaaga gattgccgta gtgctggaga aattcaaaac ctatgggtta cgaattgaag
    14341 agtaatttcg taaagcaaca aggagaagga tgatggctaa cagaatgatt ctgaacgaaa
    14401 cggcatggtt tggtcggggt gctgttgggg ctttaaccga tgaggtgaaa cgccgtggtt
    14461 atcagaaggc gctgatcgtc accgataaaa cgctggtgca atgcggcgtg gtggcgaaag
    14521 tgaccgataa gatggatgct gcagggctgg catgggcgat ttacgacggc gtagtgccca
    14581 acccaacaat tactgtcgtc aaagaagggc tcggtgtatt ccagaatagc ggcgcggatt
    14641 acctgatcgc tattggtggt ggttctccac aggatacttg taaagcgatt ggcattatca
    14701 gcaacaaccc ggagtttgcc gatgtgcgta gcctggaagg actttccccg accaataaac
    14761 ctagtgtacc gattctggca atccccacca cagcaggcac tgcggcagaa gtgaccatta
    14821 actacgtgat cactgacgaa gaaaaacggc gcaagtttgt ttgcgttgat ccgcatgata
    14881 tcccgcaggt ggcgtttatt gacgctgaca tgatggatgg tatgcctcca gcgctgaaag
    14941 ctgcgacggg tgtcgatgcg ctcactcatg ctattgaggg gtatattacc cgtggcgcgt
    15001 gggcgctaac cgatgcactg cacattaaag cgattgaaat cattgctggg gcgctgcgag
    15061 gatcggttgc tggtgataag gatgccggag aagaaatggc gctcgggcag tatgttgcgg
    15121 gtatgggctt ctcgaatgtt gggttagggt tggtgcatgg tatggcgcat ccactgggcg
    15181 cgttttacaa cactccacac ggtgttgcga acgccatcct gttaccgcat gtcatgcgct
    15241 ataacgctga ctttaccggt gagaagtacc gcgatatcgc gcgcgttatg ggcgtgaaag
    15301 tggaaggtat gagcctggaa gaggcgcgta atgccgctgt tgaagcggtg tttgctctca
    15361 accgtgatgt cggtattccg ccacatttgc gtgatgttgg tgtacgcaag gaagacattc
    15421 cggcactggc gcaggcggca ctggatgatg tttgtaccgg tggcaacccg cgtgaagcaa
    15481 cgcttgagga tattgtagag ctttaccata ccgcctggta aatgcgctga tgtgataatg
    15541 ccggatacga cgtttgcggc gtatccggct cgccgttacc gtaccaaccg caattgctgt
    15601 aaattgccgt cgatatgcaa atcggtttgt aagcgggcaa tatcgcggca cagaaacgcc
    15661 atctctttat gggtttctaa ttttttgcgc cacttttcgg caaccgcatc cagattctca
    15721 tatattcctt ccagactctg aaactcgacc agcagctgcg tggcgctttt tggtccgatt
    15781 cccacaacac ccggtacctt tgaactgctg atccccgcaa gtccccagta atcgggcaac
    15841 tgctgcggtt gaacgccaaa ttctttatcg ataaatggcg catccagcca acgtttctgg
    15901 aagtaatcac gaatacgtaa tgtcggtgaa agtaactgac agtagccttt atctgtcgaa
    15961 acaatcgttg cctgatgccc ggcctgtgtc actttgaccg ccagcgtggc ggctaagtca
    16021 tcggcttcgt tgccgctggt tgaccagcac gggacgccgc gttgctcaaa ggcggcgcgt
    16081 aatgcaggca tctcgtcgtg caactcttcc ggcattggcg gtcgacccgc tttgtaatct
    16141 ggtaaacgct gatgacgcca gccgctactg cggttttcat catcaaaaac ggcgaccgcg
    16201 tgggttggct ggctgtgcat aatgagctga tcgagcgcat gctggcaggt ttcgacacag
    16261 ggcgatccct gaacggcatg aatgcgacga ataagattca gtgcatcgac aataagcaaa
    16321 tgaacagcca cgataacccc cttaaagatc aggcgataag ggtaacattc gatgccggaa
    16381 caggctacgg ctggatgaga aatcgggaag aggcctcgca aaacgaggcc tctggagagc
    16441 gattaatcgc aggcaacgat cttcattgcc aggccgccgc gagaggtttc gcggtacttg
    16501 gcgttcatat ctttacctgt ttcgtacatg gtttcgataa ctttatcgag gcagacgcgc
    16561 ggctcgctgg tacggcgtag cgccatacgt gcggcgttca ccgcttttac tgccgcaatg
    16621 gcgttacgct cgatgcatgg cacctggacc tgtccggcga ccgggtcaca cgtcagaccg
    16681 aggttgtgct ccatggcgat ttccgccgcg atgcacacct gcgccgggct tgcacctaac
    16741 agttctgcca gaccagccgc cgccattgag cacgccacac caacttcacc ctggcagccg
    16801 acttcagcac cagaaattga cgcgttcatc ttataaagag aaccaatggc actggctacc
    16861 agcaggtaac gagccagtga gttagcgttc acttcgcgga taaacttatc gtagtacgcc
    16921 agcacagccg ggataatccc gcacgcaccg ttagtcggcg cagtcaccac gcgaccgcca
    16981 gcggcgttct cttcgttcac tgccagtgca aacatgttga tccagtcaac aaccgccatc
    17041 gggtcagtgg tggttttatc ctggctgacc agcatccggc gtagtgccgc agcacggcgt
    17101 ggtacgcgca gtttgccagg caacacgcct tcggtagaaa taccgcgctc aataccgccg
    17161 cgcatgactt cccagacgtt cgccaggtgc tgttccagct cttctttgct gtgcagcgcc
    17221 agctcgtttt tcatcatcag gccagagagt gacagcccgg tttcctgaca atgtttttgc
    17281 agatcggctg ctgaactgta cggataagga acttcaaccg gtgcgctatc ctgctggcca
    17341 aaatgttctt catcaacgat aaaaccaccg ccgatagagt agtaagtctg gctgtaaatg
    17401 actttatcgc ccgccagtgc ggtaatgcgc ataccgtttt catgcagaga aaggttgtcg
    17461 gcatggaagt tcatgcactg atcaaccggg aactccactt catgctgacc gtttgccagc
    17521 atcaggcgac catgagtatt cacatcctga ataaaaccgg ggatggaatc gatatccacg
    17581 gtatccggca ggttacccgc caggcccata ataatggcga tatcagtgtg gtggccttta
    17641 ccggtcagag agagcgagcc gtacacgtca accaccacgc gggtcacgtc tttaagcagg
    17701 ttacgggcaa tcagatcgtc ggtaaattgt ttacccgctt tcattggtcc aacggtatga
    17761 gaactggaag ggccaatgcc gattttgaaa atatcgaata cgctaatcat aggaaataca
    17821 tcgcgttaaa acggaggaag cgccgcccga aagcggcgcg aaaggactta gctgaacaga
    17881 gagtagaaga ttgcggagat tgcaatcaga cccatcacga caacgaatac gttgctgatg
    17941 tgaccgctgt acttacgcat tgccggtact ttctgaattg cgtacatcgg catcaggaac
    18001 aggatcatcg cgatgattgg gccgcccagg gtttcaatca tacccaggat gctcgggttc
    18061 agggtggcaa caatccaggt cgttaccagc atgaacagcg cagtgatacg gttcagcttg
    18121 ttgatttcga tagacttacc tttaccacgc agagatttaa tcaccatacc gttgaagcct
    18181 tcacgtgcgc ccaggtagtg gccgaggaag gatttggtga tagcgataat cgcgataatc
    18241 ggagccatcc acgcgataac cggtgcgtta aagtggttag ccaggtaaga cagaatcgag
    18301 atgttctgct ctttagccgc agccaggtct gccggagtca ggctcagtac gcagctgaag
    18361 acgaagaaca ttacggtcag caccatcatg atgtgtgcga atgccaggat cttcgagcat
    18421 ttctgttctg ccatatcgcc gtactcttca cgcttcgcaa cggcgaaaga agagatgatc
    18481 ggagagtggt tgaacgagaa caccattacc ggaattgcca gccacagggt catccacaga
    18541 ccgtttccgg ttgcagatgc agtgtccaga gacagcgttt ccagtgctgc gccgttccac
    18601 tgcgggatga ggtacagagc cagcagcatc agtacgccaa caaacgggaa taccagaata
    18661 ctcatcgctt taacgatcat ctgctcaccg aagcgaacga tggtcatcat acccacgatc
    18721 aggatcagcg acagaatcgc acgcggcggt ggcgtcatac ccagctggtg agacatgaag
    18781 ctttcaacgg tattggtgat tgccacgcta taaaccagca ggatcgggta gatagcgaag
    18841 aagtagagca gggtaatcag tttacctgcg ccaataccaa agtgttcttc tacaacctcg
    18901 gtgatgtctt cgcccgggtt tttaccagac agtacgaagc gagtcaggcc gcggtgagca
    18961 aaaaacgtca tcgggaacgc aaggatagcc atgatgatca gcgggatcat accgccaaca
    19021 ccggcgttga ttggcaggaa cagcacgccc gcgccgattg ccgtgccgta aaggcccagc
    19081 atccacatgg tgtctgtctt gcgccaggca ctacgagagt ctttcgacgc aatcgtgctg
    19141 gtttgagtcg tttccatcta tttctcctgg aggaatgcaa ataattcagc ttttagccag
    19201 ttcagcccgg gaaggtgcct gaccgtaaaa atgaaatctg tctatcggcg atggcttaat
    19261 catttaccgc cgtaataatt tgagggcgga aagatacaca caagttatgt atctatcagt
    19321 gatcttgatc ccaaatacaa taatctactt ggaagataga ttatcccaac gctttactct
    19381 gtaaatcaaa agtaaaagag aatttaaggg caatatttgc ccattaaacg acatctgact
    19441 tcttccgggg cgcgcaatat atgtaggttg aactgaaaaa atcaatactt ttagttaatc
    19501 atggcgataa tttctttttc atagcgataa aatacggttt atgaa
//
LOCUS       DAFNBJ010000089        19454 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000089 DAFNBJ010000000
VERSION     DAFNBJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.089"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..388)
                     /locus_tag="L2X49_003001"
     CDS             complement(<1..388)
                     /locus_tag="L2X49_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBN4798051.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKY"
     gene            489..1088
                     /locus_tag="L2X49_003002"
     CDS             489..1088
                     /locus_tag="L2X49_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBN4798052.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            1196..1963
                     /gene="tpiA"
                     /locus_tag="L2X49_003003"
     CDS             1196..1963
                     /gene="tpiA"
                     /locus_tag="L2X49_003003"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBN4798053.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(2018..2773)
                     /gene="cdh"
                     /locus_tag="L2X49_003004"
     CDS             complement(2018..2773)
                     /gene="cdh"
                     /locus_tag="L2X49_003004"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBN4798054.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVRKQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(2880..3869)
                     /gene="sbp"
                     /locus_tag="L2X49_003005"
     CDS             complement(2880..3869)
                     /gene="sbp"
                     /locus_tag="L2X49_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBN4798055.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEVLLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(4189..5151)
                     /gene="pfkA"
                     /locus_tag="L2X49_003006"
     CDS             complement(4189..5151)
                     /gene="pfkA"
                     /locus_tag="L2X49_003006"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBN4798056.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(5332..6234)
                     /gene="fieF"
                     /locus_tag="L2X49_003007"
     CDS             complement(5332..6234)
                     /gene="fieF"
                     /locus_tag="L2X49_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBN4798057.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(6383..6883)
                     /gene="cpxP"
                     /locus_tag="L2X49_003008"
     CDS             complement(6383..6883)
                     /gene="cpxP"
                     /locus_tag="L2X49_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBN4798058.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7033..7731
                     /gene="cpxR"
                     /locus_tag="L2X49_003009"
     CDS             7033..7731
                     /gene="cpxR"
                     /locus_tag="L2X49_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBN4798059.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7728..9101
                     /gene="cpxA"
                     /locus_tag="L2X49_003010"
     CDS             7728..9101
                     /gene="cpxA"
                     /locus_tag="L2X49_003010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBN4798060.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9207..9881)
                     /gene="yiiM"
                     /locus_tag="L2X49_003011"
     CDS             complement(9207..9881)
                     /gene="yiiM"
                     /locus_tag="L2X49_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBN4798061.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWKEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10030..11013)
                     /gene="kdgT"
                     /locus_tag="L2X49_003012"
     CDS             complement(10030..11013)
                     /gene="kdgT"
                     /locus_tag="L2X49_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBN4798062.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTTEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11273..11893)
                     /gene="sodA"
                     /locus_tag="L2X49_003013"
     CDS             complement(11273..11893)
                     /gene="sodA"
                     /locus_tag="L2X49_003013"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBN4798063.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12178..13212
                     /gene="rhaT"
                     /locus_tag="L2X49_003014"
     CDS             12178..13212
                     /gene="rhaT"
                     /locus_tag="L2X49_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HBN4798064.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13209..14075)
                     /gene="rhaR"
                     /locus_tag="L2X49_003015"
     CDS             complement(13209..14075)
                     /gene="rhaR"
                     /locus_tag="L2X49_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBN4798065.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(14131..14967)
                     /gene="rhaS"
                     /locus_tag="L2X49_003016"
     CDS             complement(14131..14967)
                     /gene="rhaS"
                     /locus_tag="L2X49_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBN4798066.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15255..16724
                     /gene="rhaB"
                     /locus_tag="L2X49_003017"
     CDS             15255..16724
                     /gene="rhaB"
                     /locus_tag="L2X49_003017"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBN4798067.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLKERKINDLPALIVATQSLPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRKTKELCA"
     gene            16721..17980
                     /gene="rhaA"
                     /locus_tag="L2X49_003018"
     CDS             16721..17980
                     /gene="rhaA"
                     /locus_tag="L2X49_003018"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBN4798068.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            18157..18981
                     /gene="rhaD"
                     /locus_tag="L2X49_003019"
     CDS             18157..18981
                     /gene="rhaD"
                     /locus_tag="L2X49_003019"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBN4798069.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWSFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            18991..19305
                     /gene="rhaM"
                     /locus_tag="L2X49_003020"
     CDS             18991..19305
                     /gene="rhaM"
                     /locus_tag="L2X49_003020"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBN4798070.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         4816 a         5121 c         4915 g         4602 t
ORIGIN      
        1 catatttatt ttcgccttgt gtgccaacaa acgcaccaag atcgataagc atcatcacca
       61 gaatcaacgt cgggacaaaa cgccccaccg cccattgcca gacacccggt aaaatcgccc
      121 agttacccgc cagcagcatc cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc
      181 cgcgatcatg caagcgctta acagttactg ccgctgttgg ccaaagcaag cacacaaggc
      241 aaaacgccgc ggtctgaata tcgagtaaat ttttacccgc cagtgagaaa agcaccagca
      301 tgcctgcgaa ccacaggcct atccaaatcc agaaatcacg gcgtccaata cgccctttaa
      361 atgagaataa ccattgctgt atggtcatgt aagttccttg atggttgtct tttccaggat
      421 tctacccttt tgacaagggc gacagatatc gttttaatcg gagccagtca taacaaaagg
      481 tactgtcaat gaagccaggg tgtacgctgt tttttctctt atgttctgca ttaaccgtta
      541 caacaacggc gcatgcacaa acaccagata cggcaacgac cgcgccttat ctgctggctg
      601 gagcccctac tttcgatctc tccatcagcc agtttcgaga agactttaac agccagaatc
      661 ctagcctgcc actgaacgaa tttcgtgcca tcgacagcag tcccgacaaa gccaatctca
      721 ctcgtgctgc aagtaaaatt aacgagaact tgtatgcttc tacagcgctg gagcgcggta
      781 ccttaaaaat caaaagcatt caaatgacct ggctacccat ccaggggcca gagcaaaaag
      841 cagcgaaagc gaaagctcag gaatacatgg cagcagtgat ccgcacactc accccattaa
      901 tgaccaaaac acaaagccag aaaaaactgc agtcgctgct aacggcgggg aaaaacaaac
      961 gttattacac cgagacagaa ggtgcactgc gttatgttgt cgcggacaac ggcgaaaagg
     1021 ggctgacctt cgctgttgaa ccgattaagc tggcgctatc tgaatcgctt gaaggtttga
     1081 ataaatgaca aaaagcaaag cctttgtgcc gatgaatctc tatactgttt cacagacctg
     1141 ctgccctgcg gggcggccat cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg
     1201 acatccttta gtgatgggta actggaaact gaacggcagc cgccacatgg ttcacgagct
     1261 ggtttctaac ctgcgtaaag agctggcagg tgttgctggc tgtgcggttg caatcgcacc
     1321 accggaaatg tacatcgata tggcgaagcg cgaagctgaa ggcagccaca tcatgctggg
     1381 tgcgcaaaac gtggacctga acctgtccgg cgcattcacc ggtgaaactt ctgccgctat
     1441 gctgaaagac atcggcgctc agtacatcat catcggccac tctgaacgtc gtacttacca
     1501 caaagagtct gacgaactga tcgcgaaaaa attcgcggtg ctgaaagagc agggcctgac
     1561 tccggttctg tgcatcggtg aaaccgaagc tgaaaacgaa gcgggcaaaa ctgaagaagt
     1621 ttgcgcacgt cagatcgacg cggtactgaa aactcagggt gctgcggcat tcgaaggtgc
     1681 ggttatcgct tacgaacctg tatgggcaat cggtactggc aaatctgcaa ctccggctca
     1741 ggcacaggct gttcacaaat tcatccgtga ccacatcgct aaagttgacg ctaacatcgc
     1801 tgaacaagtg atcattcagt acggcggctc tgtaaacgcg tctaacgctg cagaactgtt
     1861 tgctcagcca gatatcgacg gcgcgctggt tggcggtgct tctctgaaag ctgacgcttt
     1921 cgcagtgatc gttaaagctg cagaagcggc taaacaggct taagtctgac aggtgccgga
     1981 tttcatatcc ggcacttact ttccttaact cttcgcctta acgcaaaatc tcacactgat
     2041 gatcctgaat ttcctcggct gaagcacggt taagcgtcag tagatttcgt tgtgtcgcca
     2101 gcaatacaaa tgagttatca ctctgccgta ccatcgccag cccgtagctt cccatatgtt
     2161 cccgcgcctc aggtacttct tctgccagca tcataaatgg gctgcgttgt accagttcgc
     2221 tttccgttac ccgacgcgcc aggtattcat gcccgcgcaa accacctggc agtggcaacc
     2281 agcggctgct gatgttcgcc agattgttat ccagttgttt gcgcacatca ggacgaatac
     2341 aagagatatg aatatgaaaa tggttttgcg tacgcccggt gcgggagttg atcgccaaag
     2401 aaaccgcgcg atcgggaacc ggctggccgt attttttgct cataaaatca cgcgcctgcc
     2461 aggccaacca aaagaagttc ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt
     2521 taatacgata cgttggcatc aacagatatt gcagtgggcc attaagatct tttaaaacca
     2581 cgtatccggc attgggtttg acttccgcac atggcgaagg attttgattt tgctgctgat
     2641 tgggcaaaca ttcctcaagg acaatcttac gtaatgtatc cgactcttca ccggttaatt
     2701 tccagtaacc aataccggca gccacaacgg cgataactat catcaccaaa aaaagaagac
     2761 ccgctttttt catctttttt tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg
     2821 tgacaaataa aaaacccggt cgcgagaaac caccgggttt gataattatc ctggagaaat
     2881 cagcgtttgc tgatctgatc gaacgtaccg ccgttagcaa aatgctcttt ttgcgctttc
     2941 gtccagccgc cgaactcttc atcaatggtg aataacttca gctttggaaa cgcattttcg
     3001 tactttttcg ctacttcagc gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga
     3061 ccttctggcg agtagagata tttcaggtag gcttccgcca cctctttggt gccttttttc
     3121 tcgaccactt tatcgaccac cgacacggtt ggctctgcga ggatagactc actcggcgtg
     3181 acgatttcga atttatcttt ccccagttca ttcgctgcca gcagaacttc gttttcccaa
     3241 gcgatcagta catcgccaat cccgcgctcg acaaaggtgt tagtggagcc gcgcgcgcca
     3301 gaatccagaa cttcgacgtt tttatacagt gcccgaacaa aatcctgtgc ttttgcctga
     3361 tcgttgttgt tgtgatgcag cgcgtagccc caggcagcca ggtagttcca gcgtgcgcca
     3421 ccagagcttt tcggattagg cgtgatcacc gaaacacccg gtttaatcag atcgttccag
     3481 tcatggatct gcttcggatt gcccttacgc accaggaaaa caatggtgga agtgtacggt
     3541 gcggagttat ccggcagacg tttgatccac tctttatcaa tccgcccgcg ttccgcaatc
     3601 gcgtccacgt cataggccag agccagcgtg acaacatcag cttcaatacc gttgattacc
     3661 gacgtcgctt gtttacctga accaccgtgt gactgacgaa tcaccacgtt atcaccagtt
     3721 tgctgtttcc agtgggcgct gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga
     3781 tcatatgaaa cgttaagaag ctgaatatcc tttgccataa cgctggttgc cgccagcaaa
     3841 aatgttaacc ctacgcccca cttgttcatc gcccgactct cttatgttgt gttgtgatga
     3901 gcaaagcgtg ccagaaggtt aaccaaacat taaagaataa aaaaagattg gctataactt
     3961 gcgggtatat gttgagggat taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag
     4021 tgcgctttgt ccatgccgga tgcggcgcga acgccttatc cggcctacaa aagtttgcaa
     4081 attcaataaa ttgcagaatt catgtaggcc tgataagcga agcgcatcag gcatttttgc
     4141 ttctgtcatc ggtttcaggc taaaggaatc tgcctttttc cgaaatcatt aatacagttt
     4201 tttcgcgcag tccagccagt cacctttgaa cggacgcttc atgttttcga tagcgtcgat
     4261 gatgtcgtgg tgaaccagct gttcgttctg gatacctaca caacgaccgc cgtaacctgc
     4321 cagcagcaga tcgatagcgt aagcgcccat acgggaagcc agaatacggt cgtaaggcac
     4381 cggagaacca ccgcgctgga tgtggcccag cacagttgcg cgggtttcac gaccggtttc
     4441 tttctcgatg aaatgcgcca gttcgtcaac atcacacata tgttcggtaa tcgccacgat
     4501 cgcgtgtttt ttacctttcg cgatacccgc tttgatttcg tttaccaggt cttcacggct
     4561 gaattcaact tccggaacca caacgaattc acagccaccg gcaatggccg cagccagggt
     4621 cagatcgcca caataacggc ccatcacttc caccacggaa atacgctggt gagaagaaga
     4681 ggtgtcacgc agacggtcga tcgcttctac aacggtgctc agcgcagtga agaaaccgat
     4741 agtgtagtca gtgcctttga tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa
     4801 gcccatttcg gtcagacgca ttgcacccat gtaggaaccg tcaccgccga taaccaccag
     4861 cgcgtcgatc ccacgttttt tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg
     4921 gaattccggg aaacgcgcag aaccgaggaa cgtaccgcca cggttgatca tgtcagacac
     4981 gctgtaacgg tctagctgta ccatacggtc ttcatacaga cccagatagc cgtcataaat
     5041 acccattact tccagacctt ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt
     5101 catgcctggc gcatcaccgc cgcttgtcaa cacaccgatt ttcttaatca tgactacctc
     5161 tgaactttgg aatgcaaaat aaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat
     5221 ccaacgaatg tgcaaatagt ataacaatca cttcctgctg aattgattca ggtcaggcca
     5281 aatggcggta ttttatacac aaaatgcggg tctggctctc ttttatactg attatgaaag
     5341 catagaccgt ttaccctccc tgggtacgac ggaacagggg tcctgatgga taatcacatc
     5401 cgatcccgga aaacgccgta aaatagcctg ctctacctga tccgccacca tatgtgcctg
     5461 aaccaaaggc agagagtctt ccatttccaa atgaatctga ataaagcggg tcggccctga
     5521 ctgccgcgtg cgaagatcgt gagcgccgct aacacccggc caggaagtca cgatatcaat
     5581 aatttcttgc cgttcctcat caggcaatgc gcgatccagt aatgactgta ccgcctcata
     5641 tcccatgcgt aacgcgctat ataaaatata gatgccgatt cccaatgcaa acagagcatc
     5701 ggcgcgatgc cagccgtacc aggacaaccc cagcgccagc agaattgcgc cgttcatcat
     5761 aacatcagac tggtaatgta gcatatcagc cctcaccgcc tggctttgcg tccggcgcac
     5821 cacccaacgc tgaaacgaga caaggataat cgtacaaatt agcgccacaa ttgtcacgat
     5881 aaccccgacg cctggatctg tcatcggtgt tggagatacc agatgttgaa tacccgtcaa
     5941 aaacaggaat agtgccgaac cggagataaa catactttgc gccagcgccg cgagggactc
     6001 agctttaccg tgaccaaacg agtgattatc gtcggcaggt tgcagggaat atcgcaccac
     6061 cagtaaattc gtcaacgacg cgccgatatc caccagcgaa tccaccagcg cggcgagaat
     6121 actcaccgac ccggtatacc accatgcaaa aattttaatc agcaatagca gcgaagccat
     6181 cgccgtcgca gcaatcgccg cccgactgac cagccgtcca taagattgat tcataaatac
     6241 tcccgctatc aactgacgct agtataacgg aagcaaatca tctgcaatgc attaagcagc
     6301 aggcaaattg aggataaaaa aaacccccac atcatgtggg ggaagacagg gatggtgtct
     6361 atggcaagga aaacagggtt tactactggg aacgtgagtt gctactactc aatagcttca
     6421 acgatgaact tttttgccat tgcgtcacgt cgcgcaactg ctccattcgt tgttgatgtt
     6481 tctcgtttaa aaccgcttgc tgctccggcg ttaacaggcg atacatttgg ttgcggactt
     6541 ttgccatctc aacctgacga gcaatttgct cattcgccat tttttctgcc tgtgcgcgca
     6601 cagcgttttc atcaaaattt tctgcggtga caaggcgatg cattgtctcc agttcgctaa
     6661 cattaacagg aggctgttcg tgccgggcct gttgcataag atctcgcatc tgctgacgct
     6721 gatgttcggt taaacttatg ccgtcgaaca tatggctctg cgtactgcgc tgcgtaagtt
     6781 cttcacccgg atgccagtta tcgcctgaac cgacttcagc agcgtggctt aatgaactga
     6841 ctgccagcgt tgaggccatg acggcagcgg taactatgcg catcatttgc tcccaaaatc
     6901 tttctgtcgc gattcaacga tagagagttt acgattcagg ctgcaaacat gcgtcagggg
     6961 gtgtaaaaca acgtaaagtc atggattagc gacgtctgat gacgtaattt ctgcctcgga
     7021 ggtatttaaa caatgaataa aatcctgtta gttgatgatg accgagagct gacttcccta
     7081 ttaaaggagc tgctcgagat ggaaggcttc aacgtgattg ttgcccacga tggggaacag
     7141 gcgcttgatc ttctggacga cagcattgat ttacttttgc ttgatgtaat gatgccgaag
     7201 aaaaatggta tcgacacatt aaaagcactt cgccagacac accagacgcc tgtcattatg
     7261 ttgacggcgc gcggcagcga acttgatcgc gttctcggcc ttgagctggg cgcagatgac
     7321 tatctcccga aaccgtttaa tgatcgtgag ctggtggcac gtattcgcgc gatcctgcgc
     7381 cgttcgcact ggagcgagca acagcaaaac aacgacaacg gttcaccgac actggaagtt
     7441 gatgccttag tgctgaatcc aggccgtcag gaagccagct tcgacgggca aacgctggag
     7501 ttaaccggca ctgagtttac cctgctctat ttgctggcac agcatctggg tcaggtggtt
     7561 tcccgtgaac atttaagcca ggaagtgctg ggcaaacgcc tgacgccttt tgaccgcgct
     7621 atcgatatgc acatttccaa cctgcgtcgt aaactgccgg atcgtaaaga tggtcacccg
     7681 tggtttaaaa ccttgcgtgg tcgcggctat ctgatggttt ctgcttcatg ataggcagct
     7741 taaccgcgcg catcttcgcc atcttctggc tgacgctggc gctggtgttg atgttggttt
     7801 tgatgttacc caagctcgat tcacgccaga tgaccgagct tctggatagc gaacagcgtc
     7861 aggggctgat gattgagcag catgttgaag cggaactggc gaacgatccg cccaacgatt
     7921 taatgtggtg gcggcgtctg tttcgggcga ttgataagtg ggcaccgcca ggacagcgtt
     7981 tgttattggt gaccaccgaa ggccgcgtga tcggcgctga acgcagcgaa atgcagatca
     8041 ttcgtaactt tattggtcag gccgataacg ccgatcatcc gcagaagaaa aagtatggcc
     8101 gcgtggaact ggtcggtccg ttctccgtgc gtgatggcga agataattac caactttatc
     8161 tgattcgtcc ggccagcagt tctcaatccg atttcattaa cttactgttt gaccgcccgt
     8221 tattactgct gattgtcacc atgttggtca gtacgccgct gctgttgtgg ttggcctgga
     8281 gtctggcaaa accggcgcgt aagctgaaaa acgctgccga tgaagttgcc cagggaaact
     8341 tacgccagca cccggaactg gaagcggggc cacaggaatt ccttgccgca ggtgccagtt
     8401 ttaaccagat ggtcaccgcg ctggagcgta tgatgacctc ccagcagcgt ctgctttctg
     8461 atatctctca cgagctgcgc accccactga cgcgtctgca actgggtacg gcgttactgc
     8521 gccgtcgtag cggtgaaagc aaggaactgg agcgtattga aaccgaagcg caacgtctgg
     8581 acagcatgat caacgatctg ttggtgatgt cacgtaatca gcaaaaaaac gcgctggtta
     8641 gcgaaaccat caaagccaac cagttgtgga gtgaagtgct ggataacgcg gcgttcgaag
     8701 ccgagcaaat gggcaagtcg ttgacggtta acttcccgcc tgggccgtgg ccgctgtacg
     8761 gcaacccaaa cgccctggaa agtgcgctgg aaaacattgt tcgtaatgct ctgcgttatt
     8821 cccatacgaa gattgaagtg ggctttgcgg tagataaaga cggtatcacc attacggtgg
     8881 acgacgatgg tcctggcgtt agcccggaag atcgcgaaca gattttccgt ccgttctatc
     8941 ggaccgatga agcacgcgat cgtgaatctg gcggtacagg tttggggctg gcgattgttg
     9001 aaaccgccat tcagcagcat cgtggctggg tgaaggcaga agacagcccg ctgggcggtt
     9061 tacggctggt gatttggttg ccgctgtata agcggagtta aactccgcat ttgtaggcag
     9121 gataaggcgt ttacgccgca tccggcattt gagcaggatg cctgatgcga cgctgatagc
     9181 gccttatcag gcctacactc cgggggttat tttccccaca accggcggga aaaatcttcg
     9241 atcttgccgc tcagtcggcg cttttgcatc gtccgcgtcc agcttttcga taacccggcg
     9301 gcggagagta aacggtgata ttgatcgtca tcaaacggca tatgccatgc gatggcggca
     9361 gcctcctgca cggtcacatc gctgacacgg gaaaccaact ccagcggcgc gtccgcagac
     9421 actttccctg gcgctatcac gctatacaac caccccactt tacccgtgtt ttgcatcaac
     9481 tgcgctatat cgctgatatc aaaatggtaa ttgagtttat agcaaggcga gcgcggctgg
     9541 ctgacctgaa ttaatgcctc tttccagcgg aaaatatcgc ccatataaac attactttcc
     9601 gtgagaccgt cggttgagag attttcgcca aacgcaggcg caacaaacaa ctccgcctgt
     9661 tccggaaatt cccgcgccca gtagagatag tgctcacgag gataatgaca cagcgctctg
     9721 tctggcccgc cgtgaacctt tttctccgcc tgctcgtcac cttccagccc cagctccgtc
     9781 agcatcaact caccatcaac ctggattttg gcaattgcgc tgggtttgcc ttcgggataa
     9841 gcctgaatct tgcctgtgta tacatcaacc ggatatcgca tctgttccct ctccacgaga
     9901 aatttcccca ttagaccata tttattttat gtcaggcgtg ctgttttcgc ggtaagtatc
     9961 caggcaggaa ctcaatagca aaccggaaga acgttagttc atccggctgt ggagattaag
    10021 aaagcaggtt tatttcaccg tacctaaaat ttcgattttc gctgctctgg ctttgacttt
    10081 acgtgaccag atagaggtaa gaattggcac cagaatcgaa gtcacaatga ccgccgtcgc
    10141 taccagcgaa gttgctgccg gagccatcgg tttaaacgca ggcaccattt ctgcaatcag
    10201 cacaggtgtc gctaccgcgg cccctgcgga actggaagcg gcaattccgg cagtgccatc
    10261 gccaccgcca atcaatttat cggcgataat caacggaata ccggtcacga taattactgc
    10321 cacacccaac aggatcccca gcaaaccagt ctgggcaatc acagtcaaat caatggtatt
    10381 gcccagcgcg aaggcaaaga atggaatcag cgtttgcacc gctttgctga aaaattctcg
    10441 taattcaggg tcaaggttcc ccagggcaaa gcccaccagg aacggtaata ctgcgccgac
    10501 gaaaacatgc ggttcaaacg aggcaatccc tgcagtgccc agaataatca tcgtcatgag
    10561 cggcccggac tccaacgaca tcaacacaaa tgccccagct tcttctgttg tgccgtactg
    10621 ctgcatgatg gaagcgtaaa gtccgccatt ggtcatatcc atcgccgcca ccagcgccag
    10681 cgttgaaagt ccggcaaaga atccaacttc aacaccatgt tccggaataa tgcgcgaggc
    10741 aattgccgca accacccacg cgacggcaat ttttgttacc accagcgtac cggatttacg
    10801 caataccgtt cccgtcgcgc ttaattttat tgacgccccc atgcaaaaaa accacaccgc
    10861 cagaatgggc accgtaccgg taatcatccc gttggtgaat gatccaaaat atttccccgc
    10921 ccccggcgag aaggtgtggc acagtgcgcc aaggaatagc gggacgagca tcatcccccc
    10981 cgggattttc tcaatcgagc gttttatctg catttccatc acctgtcggc atatttttaa
    11041 gggctgatgc gtcattcctc aatggagcat cagtgttgat ggaaacgaag atagacattc
    11101 atttaatcaa taaaagtgat caaaaccaca tcaattgaaa cactgtttta tttttatcgg
    11161 atcattgttt ttattttgaa attgatcaca aaaaaaccac cgccgttggc gatggttcat
    11221 tttttaagct gatatgcggc ctatacgcct cattgcagca ggcggcaaat gattattttt
    11281 tcgccgcaaa acgtgccgct gcttcgtccc agttcaccac gttccagaac tctttaatgt
    11341 agtccggacg gcggttctgg aatttcaggt agtaagcatg ttcccacaca tccaggccca
    11401 gaatcgggaa gccggaagcg ccagaaatag cttcacccat cagcggagaa tcctggttag
    11461 cagtagaaac caccgccagt ttatcgcctt tcagcaccag ccatgcccag ccggaaccaa
    11521 agcgggaagc tgccgctttt tcaaattctg ctttgaagtt atcaacggag ccgaagtcac
    11581 gttcgatagc cgctttcagg tcaccctgca gggtggtgcc ttttttcaga cctttccaga
    11641 acaggctgtg gttagcgtga ccgccagcgt tgttgcgcag tacggttttc ttgtctgctg
    11701 gcagctggtc cagtttggtg atcagctctt caaccggcag gttggcaaat tctggcaggc
    11761 tttccagcgc cgcgttggcg ttgtttacgt aggtctgatg gtgtttggtg tggtggattt
    11821 ccatggtctg cttatcgaag tgcggttcca gggcatcgta agcatacggc agggatggca
    11881 gggtatagct catattcatc tccagtattg tcgggcggcc gattgttaat gccgcgtaag
    11941 cagttggttc attatagtta attaaatgat attgaaaatg attatcaatg ccgtactttt
    12001 cgtaagggta tggttttgca ggaaaatgcc cgagatgtga agcaaatcac ccacttaatg
    12061 ccgtgattgc cagtaaatcg acaacggcgg caacaggcga aaggttaatc gacagcacga
    12121 tttttacact catctcgtcg gagatgtgaa gcgacgaaaa atgatgagga taagaagatg
    12181 agtaacgcga ttacgatggg gatattttgg catttgatcg gcgcggccag tgcagcctgt
    12241 ttttacgctc cgttcaaaaa agtaaaaaaa tggtcatggg aaaccatgtg gtcagtcggt
    12301 gggattgttt cgtggattat tctgccgtgg gccatcagcg ccctgttact accggatttc
    12361 tgggcgtatt acagctcgtt tagtctctct acgctgctgc ctgtttttct gttcggcgct
    12421 atgtggggga tcggtaatat caactacggc ctgaccatgc gttatctcgg catgtcgatg
    12481 ggaattggca tcgccattgg cattacgttg attgtcggta cgctgatgac gccaattatc
    12541 aacggcaatt tcgatgtgct gattaacacc gaaggcggac gcatgacgtt gctcggcgtt
    12601 ctggtggcgc tgattggcgt agggattgta actcgagccg ggcagttgaa agagcgcaag
    12661 atgggcatta aagccgaaga gttcaatctg aaaaaagggc tggtgctggc ggtgatgtgc
    12721 ggcattttct ctgccggtat gtcttttgcg atgaacgccg caaaaccgat gcatgaagcc
    12781 gctgccgcac ttggcgtcga tccactgtat gtcgctctgc caagctatgt catcatcatg
    12841 ggcggcggcg cgatcatcaa cctcggtttc tgcttcattc gtctggcaaa agtgaaggat
    12901 ttgtcgctaa aagccgactt ctcgctggca aaaccgctga tcattcacaa tgtgttactc
    12961 tcagcactgg gcgggttgat gtggtatctg caattctttt tctatgcctg gggccacgcc
    13021 cgcattccgg cgcagtatga ctacatcagt tggatgctgc atatgagttt ctatgtattg
    13081 tgcggcggta tcgtcgggct ggtgctgaaa gagtggaaca atgcaggtcg ccgtccggta
    13141 acggtgttga gcctcggttg tgtggtgatt attgtcgccg ccaacatcgt cggcatcggc
    13201 atggcgaatt aatctttctg cgaattgaga tgacgccact ggctgggcgt catcccggtt
    13261 tcccgggtaa acaccaccga aaaatagtta ctatcttcaa agccacattc ggtcgaaata
    13321 tcactgatta acaggcggct atgctggaga agatattgcg catgacacac tctgacctgc
    13381 cgcagatatt gattgatggt cattccagtc tgctggcgaa attgctgacg caaaacgcgc
    13441 tcactgcacg atgcctcatc acaaaattta tccagcgcaa agggactttt caggctagcc
    13501 gccagccggg taatcagctt atccagcaac gtttcgctgg atgttggcgg caacgaatca
    13561 ctggtgtaac gatggcgatt cagcaacatc accaactgcc cgaacagcaa ctcagccatt
    13621 tcgttagcaa acgacacatg ctgactactt tcatgctcaa gctgaccgat aacctgccgc
    13681 gcctgcgcca tccccacgct acctaagcgc cagtgtggtt gccctgcgct ggcgctaaat
    13741 cccggaatcg ctccctgcca gtcaagattc agcttcagcc gctccgggca ataaataata
    13801 ttctgcaaaa ccagatcgtt aacggaagcg taggagtgtt tatcgtcagc atgaatgtaa
    13861 aagagatcgc cacgggtaat gcgataaggg cgatcgttga gtacatgcag accattaccg
    13921 cgccagacaa tcaccagctc acaaaaatca tgtgtatgtt cggcaaaaac atcttgcgga
    13981 taacggtcag ccacagcgac tgcctgctgg tcgctggcaa aaaaatcatc tttgagaagt
    14041 tttaactgat gcgccaccgt ggctacctcg gccagagaac gaagttgatt attcgcaata
    14101 tggcgtacaa atacgttgag aagattcgcg ttattgcaga aaaccatccc gtccctgacg
    14161 aatatcacgc ggtgaccagt taaactctcg gcgaaaaagc gtcgaaaagt ggttactgtc
    14221 gctgaatcca cagcgatagg cgatatcagt aacgctggcc tcgctgtggc gtagtagatg
    14281 tcgggctttc atcagtcgca ggcggttcag gtaacgctga ggcgtcagtc ccgtttgctg
    14341 cttaagctgc cgatgtagcg tacgcagcga aagagaaaat tgctccgcca cggcatccca
    14401 attcacctca tcggcaaaat ggtcctccag ccaggccaga agcaagttga gacgtgatgc
    14461 gctgttttcc aggttctcct gcaaactgct tttacgcagc aagagcagta attgcataaa
    14521 caagatctcg cgactggcgg tcgagggtaa atcattttcc ccttcctgct gttccatctg
    14581 tgcaaccagc tgtcgcacct gctgcaatac gctgtggtta acgcgccagt gggaaggata
    14641 ctgcccatcc agctcttgtg gcagcaactg attcagcccg gcgagaaact gaaatcgatc
    14701 cggcgagcga tacagcacat tagtcagaca cagattatcg gtatgttcat acagatgccg
    14761 atcatggtcg cgcacaaaac agaccgtgcc accggtgatg gtatagggct gcccattaaa
    14821 cacatgaata cccgtgccat gttcgacaat cacaatttca tgaaaatcat gatgatgttc
    14881 aggaaaatcc gcctgcggga gccggggttc tatcgccacg gacgcgttac cagacggaaa
    14941 aaaatccaca ctatgtaata cggtcatact ggcctcctga tgtcgtcaac acggcgaaat
    15001 agtaatcacg aggtcaggtt cttaccttaa attttcgacg gaaaaccacg taaaaaacgt
    15061 cgatttttca agatacagcg tgaattttca ggaaatgcgg tgagcatcac atcaccacaa
    15121 ttcagcaaat tgtgaacatc atcacgttca tctttccctg gttgccaatg gcccattttc
    15181 ctgtcagtaa cgagaaggtc gcgaattcag gcgcttttta gactggtcgt aatgaaattc
    15241 agcaggatca cattatgacc tttcgcaatt gtgtcgccgt cgatctcggc gcatccagtg
    15301 ggcgcgtgat gctggcgcgt tacgagcgtg aatgccgcag cctgacgctg cgcgaaatcc
    15361 atcgttttaa caatgggctg catagtcaga acggctatgt cacctgggat gtggatagcc
    15421 tggaaagtgc cattcgcctt ggattaaaca aggtgtgcga ggaagggatt cgtatcgata
    15481 gcattgggat tgatacctgg ggcgtggact ttgtgctgct cgaccaacag ggtcagcgtg
    15541 tgggcctgcc cgttgcttat cgcgatagcc gcaccaatgg cctgatggcg caggcacaac
    15601 aacaactcgg caaacgcgat atttatcaac gtagcggcat ccagtttctg cccttcaata
    15661 cgctttatca gttacgggcg ctgacggagc aacaacctga acttattcca cacattgctc
    15721 acgctctgct aatgccggat tattttagtt atcgccttac cggcaagatg aactgggagt
    15781 acaccaacgc cacaaccacg caactggtca atatcaatag cgacgactgg gacgagtcgc
    15841 tactggcgtg gagcggtgcc aacaaagcct ggtttggtcg cccgacgcat ccgggtaatg
    15901 tcataggtca ctggatttgc ccgcagggta atgagattcc ggtggtcgcc gttgccagcc
    15961 atgataccgc cagcgcggtt atcgcctcgc cgttaaacgg ttcacgcgcc gcttatctct
    16021 cttctggtac ctggtcattg atgggcttcg aaagccagac gccatttacc aatgacacgg
    16081 cgctggcagc caacatcacc aatgaaggcg gggcggaagg tcgctatcgg gtgctgaaaa
    16141 atattatggg cttatggctg cttcagcggg tgcttaagga gcggaaaatc aacgatctcc
    16201 cggcgcttat cgtcgcgaca cagtcacttc cggcctgccg cttcatcatc aatcccaatg
    16261 acgatcgctt tattaatcct gaggcgatgt gcagcgaaat tcaggctgcg tgtcgggaaa
    16321 cggcgcaacc gatcccggaa agtgatgctg aactggcgcg ctgcattttc gacagtctgg
    16381 cgctgctgta tgccgatgtg ttgcatgagc tggcgcagct acgcggtgaa gatttctcgc
    16441 aactgcatat tgtcggcgga ggctgtcaga acacgctgct caaccagcta tgtgccgatg
    16501 cctgcggtat tcgggtgatc gccgggcctg ttgaagcctc gacgctcggc aatatcggca
    16561 tccagttaat gacgctggat gaactcaaca atgtggatga tttccgtcag gtcgtcagca
    16621 ccaccgcgaa tctgaccacc tttaccccta atcctgacag tgaaattgcg cactacgtgg
    16681 cgcggattca ctctacacga aagacaaagg agctttgcgc atgaccactc aactggaaca
    16741 ggcctgggag ctggcgaaac agcgtttcgc ggcggtgggg attgatgtcg aggaggcgct
    16801 gcgccaactt gatcgtttac ccgtttcaat gcactgctgg cagggcgatg atgtttccgg
    16861 ttttgaaaac ccggaaggtt cgctgaccgg ggggattcag gccacaggca attatccggg
    16921 caaagcgcgt aatgccagtg agctacgtgc cgatctggaa caggctatgc ggctgattcc
    16981 ggggccgaaa cggcttaatt tacatgccat ctatctggaa tcagatacgc cagtctcgcg
    17041 cgaccagatc aaaccagagc acttcaaaaa ctgggttgaa tgggcgaaag ccaatcagct
    17101 cggtctggat tttaacccct cctgcttttc gcatccgcta agcgccgatg gctttacgct
    17161 ctcgcatccc gacgacagca ttcgccagtt ctggattgat cactgcaaag ccagccgtcg
    17221 cgtttcggcc tattttggcg agcaactcgg cacaccatcg gtgatgaaca tctggatccc
    17281 ggatggtatg aaagatatca ccgttgaccg tctcgccccg cgtcagcgtc tgctggcagc
    17341 tctggatgag gtgatcagcg agaagctgga tccggcgcac catatcgacg ccgttgagag
    17401 caaattgttt ggcattggcg cagagagcta cacggttggc tccaatgagt tttacatggg
    17461 gtatgccacc agccgccaga ctgcgctatg cctggacgcc gggcacttcc acccgactga
    17521 agtgatttcc gacaagattt ccgccgccat gctgtatgtg ccgcagttgc tgctgcacgt
    17581 cagccgtccg gttcgctggg acagcgatca cgtagtgctg ctggatgatg aaacccaggc
    17641 aattgccagt gagattgtgc gtcacgatct gtttgaccgg gtgcatatcg gccttgactt
    17701 cttcgatgcc tctatcaacc gcattgccgc gtgggtcatt ggtacacgca atatgaaaaa
    17761 agccctgctg cgtgcgttgc tggaacctac cgctgagctg cgcaagctgg aagcggcggg
    17821 cgattacact gcgcgtctgg cactgctgga agagcagaaa tcgttgccgt ggcaggcggt
    17881 ctgggaaatg tattgccaac gtcacgatac gccagcaggt agcgaatggc tggagagcgt
    17941 gcgggcttat gagaaagcga ttttgagcca gcgtgggtaa acactgccgg atgcggcgcg
    18001 agcgccttat ccggcctaca ggtcggcaac agttgtaggc ctgataagac gcgacagcgt
    18061 cgcatcaggc attgattgcc ggatgcgacg ctgtcgcgtc ttatccggcc tacgggtcgt
    18121 gcatctgaca acaccgaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg
    18181 tttgtccagg gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc
    18241 aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac
    18301 ttccacccac aaccgcgcta tattccgctc agccagccca tgcctttact ggcaaatacg
    18361 ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagcttga tcctgcagct
    18421 aacttaggcg tcgtaaaagt cgacagcgac ggcgcgggct accatattct ctgggggtta
    18481 accaacgaag ccgttcccac ttccgaactt ccggctcact tcctttccca ctgcgagcgc
    18541 attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc
    18601 gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc
    18661 agcaccgagt gtctggtagt gttcccggat ggcgttggca ttttgccgtg gatggtgccc
    18721 ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg
    18781 tggtccttcc acggcgtctt cggcagcggg ccgacgctgg atgaaacatt cggtttaatc
    18841 gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa
    18901 cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta
    18961 gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggtt
    19021 aaccccgacg cccacgaaga gtatcagcgt cggcataacc ccatctggcc ggaactggaa
    19081 gcagtgttga aatctcacgg tgcgcataac tacgccattt acctcgacaa agcgcgtaat
    19141 ctgctgtttg ccacggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact
    19201 gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccggataac
    19261 agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg
    19321 gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc
    19381 ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca
    19441 ttaatgattc atgt
//
LOCUS       DAFNBJ010000090        19201 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000090 DAFNBJ010000000
VERSION     DAFNBJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.090"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(48..424)
                     /gene="rnpB"
                     /locus_tag="L2X49_003021"
     ncRNA           complement(48..424)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="L2X49_003021"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(457..1602)
                     /gene="garK"
                     /locus_tag="L2X49_003022"
     CDS             complement(457..1602)
                     /gene="garK"
                     /locus_tag="L2X49_003022"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBN4798071.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1699..2589)
                     /gene="garR"
                     /locus_tag="L2X49_003023"
     CDS             complement(1699..2589)
                     /gene="garR"
                     /locus_tag="L2X49_003023"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBN4798072.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2619..3389)
                     /gene="garL"
                     /locus_tag="L2X49_003024"
     CDS             complement(2619..3389)
                     /gene="garL"
                     /locus_tag="L2X49_003024"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBN4798073.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     IDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     IEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3405..4739)
                     /gene="garP"
                     /locus_tag="L2X49_003025"
     CDS             complement(3405..4739)
                     /gene="garP"
                     /locus_tag="L2X49_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBN4798074.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWSVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALIFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5114..6685
                     /gene="garD"
                     /locus_tag="L2X49_003026"
     CDS             5114..6685
                     /gene="garD"
                     /locus_tag="L2X49_003026"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBN4798075.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNIGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6834..7169
                     /gene="prlF"
                     /locus_tag="L2X49_003027"
     CDS             6834..7169
                     /gene="prlF"
                     /locus_tag="L2X49_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HBN4798076.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7169..7633
                     /gene="yhaV"
                     /locus_tag="L2X49_003028"
     CDS             7169..7633
                     /gene="yhaV"
                     /locus_tag="L2X49_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HBN4798077.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(7688..8497)
                     /gene="agaR"
                     /locus_tag="L2X49_003029"
     CDS             complement(7688..8497)
                     /gene="agaR"
                     /locus_tag="L2X49_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBN4798078.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8746..10026
                     /gene="kbaZ"
                     /locus_tag="L2X49_003030"
     CDS             8746..10026
                     /gene="kbaZ"
                     /locus_tag="L2X49_003030"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBN4798079.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10049..10522
                     /gene="agaV"
                     /locus_tag="L2X49_003031"
     CDS             10049..10522
                     /gene="agaV"
                     /locus_tag="L2X49_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBN4798080.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10533..11312
                     /locus_tag="L2X49_003032"
     CDS             10533..11312
                     /locus_tag="L2X49_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBN4798081.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11302..12180
                     /locus_tag="L2X49_003033"
     CDS             11302..12180
                     /locus_tag="L2X49_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBN4798082.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12198..12632
                     /locus_tag="L2X49_003034"
     CDS             12198..12632
                     /locus_tag="L2X49_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN4798083.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12629..13762
                     /gene="nagA"
                     /locus_tag="L2X49_003035"
     CDS             12629..13762
                     /gene="nagA"
                     /locus_tag="L2X49_003035"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBN4798084.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEKVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14113..15267
                     /locus_tag="L2X49_003036"
     CDS             14113..15267
                     /locus_tag="L2X49_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HBN4798085.1"
                     /translation="MPKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAASPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15280..16140
                     /gene="kbaY"
                     /locus_tag="L2X49_003037"
     CDS             15280..16140
                     /gene="kbaY"
                     /locus_tag="L2X49_003037"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HBN4798086.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16307..16783
                     /gene="agaB"
                     /locus_tag="L2X49_003038"
     CDS             16307..16783
                     /gene="agaB"
                     /locus_tag="L2X49_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBN4798087.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            16822..17625
                     /gene="agaC"
                     /locus_tag="L2X49_003039"
     CDS             16822..17625
                     /gene="agaC"
                     /locus_tag="L2X49_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HBN4798088.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17615..18406
                     /gene="agaD"
                     /locus_tag="L2X49_003040"
     CDS             17615..18406
                     /gene="agaD"
                     /locus_tag="L2X49_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HBN4798089.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18407..19162
                     /locus_tag="L2X49_003041"
     CDS             18407..19162
                     /locus_tag="L2X49_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HBN4798090.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         4633 a         5012 c         4916 g         4640 t
ORIGIN      
        1 gctcctccaa aagcaaaaac ccgccgaagc gggtttttac gtaaatcagg tgaaactgac
       61 cgataagccg ggttctgtcg tggacagtca ttcatctagg ccagcaatcg ctcactggct
      121 caagcagcct acccgggttc agtacgggcc gtaccttatg aacccctatt tggccttgct
      181 ccgggtggag tttaccgtgc cacggactgt taccagccgc gcggtgcgct cttaccgcac
      241 cctttcaccc ttacctgatc ccgcttgcgc gggccatcgg cggtttgctc tctgttgcac
      301 tggtcgtggg tttccccccc aggcgttacc tggcaccctg ccctatggag cccggacttt
      361 cctcccctcc gcccgtctcc cccgaagagg acgacgacga agcggcgact gtctggtcag
      421 cttcggcgcg cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc gcattccaat
      481 cgccagtgtc gcggcgatat tacgtgaagc acggcagata ttgtcataag ccccgcggaa
      541 tgcttcgtcc aacgtaccta tgctggtcaa tacgctgaag accgcatcga tgccatgctg
      601 atgtacaacg ccaacatcat cggtcaggct acccgcaatg ccaatcaccg gtttatggta
      661 cttcttcgcc acgtttgcaa cgccaatcgg taccttgccg tgaatactct ggctgtcaat
      721 acgcccttca ccggtgatca ccagcgtaca atcgtgaata tgttcctcca gattcagcgc
      781 cgtagtgacg atttcaatac cacttttcag ttccgcacca agaaacgcca tcagcgccgc
      841 acccataccg cccgcagctc ctgcgccggg gacatctttc acatcaacat gcagcgcttt
      901 tttaatgaca tcggcatagt gagagaggtt attgtccagc tcaacaatca tcgcttcact
      961 ggctcccttt tgtgggccaa agatgcgcga tgcgccgtta tcgcccacca gcggattggt
     1021 gacatcacaa gcgacgcgaa tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc
     1081 aatatcattc agagtattaa gactaccgcc gccaaaacca atttcattgc cgttggcgtc
     1141 gcataatttc gcccccagcg cctgtaccat gcctgcgccg ccatcatttg tagcgctacc
     1201 gccaatgccg ataataatgt ttgtcgcgcc gctctccagc gcctgcagga ttaactcgcc
     1261 tgtgccgcgt gaagtggtca cgagtggatc gcgtttttcc gcaggtacca gctccagccc
     1321 actggccgcc gccatttcaa taaacgcggt tttgccatcg ccggagatcc cccaactggc
     1381 attcactttc tcgcccagcg gccctgtaac ccaggcgtga cgttcagaac cctgggtggc
     1441 tgcaatcatc gcttccaccg ttccttcgcc accgtcggca accggaatag aaacgtactg
     1501 tgcatcagga aaaatttccc gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga
     1561 taaactttct ttataagagt ctggggcgat tacgattttc atacctatgc ctgttaccac
     1621 atgacgccgg agggcgtttc tcttattcgg cctggattcc aggcccggat tgcaatacgc
     1681 catccgggca cgacgtcatt aacgagtaac ttcgactttc gccagttttt cgtagtagca
     1741 cgccagggcg ctatgatccg ccgttcctaa accatctgct cgcagtgcct gcatcatctc
     1801 cataaccgca gctgtgagcg gcagttgcgc gccgacgccg tgggaagtat ccagcgcatt
     1861 tgccagatcc ttaatatgca gatcaatacg gaagcccggc ttgaagttgc ggtccatcac
     1921 catcggcgct ttggcatcca gcacggtact gcccgccagt ccaccgcgaa ttgcctgata
     1981 caccaggtcc gggttaacgc ccgctttggt tgccagcgtt agcgcttctg acatcgcggc
     2041 aatattcagc gccacaatga cctgatttgc cagtttggtg acgttacctg caccgatttc
     2101 cccggtatgc accacggaac ccgccatcgc tttcatcaaa tcatagtatt tgtcgaaaat
     2161 agccttgtcg ccgcccacca tcactgacag cgtaccgtcg atggctttcg gttcaccgcc
     2221 gctcaccgga gcatccagca tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg
     2281 gcttgccagc ggtgcgatag aactcatatc gatcaatacc gtacctggct tcgcgccttc
     2341 aatgatgcca ttctcaccca gcgccacctc tttcacatga ggggagtttg gcagcatggt
     2401 tatgatgacg tcgcactgtt cagcgatcgc tttagccgta gacgctgttt ctgcacctgc
     2461 agcaatcacg tcagcaatag cttctgggtt acggtcagca accaccagcg agtaacctgc
     2521 tttcagaagg tttttactca ttggtttacc cataatcccc aggccaataa aaccaacttt
     2581 catagtcata tcaatcatct ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc
     2641 agtttctgag tggcagagcg gaagacgccg agatcgctgc cgacagccac aaacgtcgcg
     2701 ccccattcca gataacgacg cgcatcggct tcgatcggtg cgaggatacc gctgggtttg
     2761 ccgtgcgcac tggcacggtt aaaaatgtgc tgaattgctt tttgtacatc cgggtgcgag
     2821 gcattgccga gatggcctaa tgccgcagcc agatcgctgg ggccgacgaa gatgccgtct
     2881 acgccttcgg tagcggcaat ggcatcgatg ttatctacgc cctgctgact ttctatctgg
     2941 accagaatag tgatgttctt gttcgactga gcgaaataat ccgccacggt gccaaacata
     3001 ttggcgcggt gagaaacgga gacgccgcga atgccttccg gtgggtaacg ggttgatgcc
     3061 accgcctgct ctgcttcctc ttttgtttct acaaaaggaa tcaggaagtt atagaaaccg
     3121 atatccagaa gacgcttaat aattaccggc tcgttggtcg gcactcgcac tactggcgcg
     3181 ctggcgctgc ctttcagggc cattaactgc ggaataaacg tggaaatatc gtttggcgca
     3241 tgttcgccat ccagcaccag ccagtcaaac ccagccaaac caagaacttc agtgctaatc
     3301 gggttagaga gtgctgacca gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg
     3361 aatttattcg ggaaaacatc gttattcatc gcttatacct ttgcttattt ctgcaattcc
     3421 atacgtttaa tgtcgccaac tacgaagagg tagcagacca tcgccatcag cgctgaacat
     3481 cccacgaaaa tcagtgctgc attgaaggag tgcagttcac ttaccaggta gccaatcacc
     3541 agtggagtga caatggaggc aacattgcca aagacgttaa agacgccgcc gcagaggcca
     3601 acaatctctt tcggcgcggt gtcagaaatc accgaccagc ccagagcacc aaatcctttg
     3661 ccaaaaaaag ccagcgccat cagcatgacc accagcgtgg tgttgttggt gtagttacat
     3721 aagatgatgg tggaagccag caacattccc agcacaatcg gtagcttacg tgccagggtc
     3781 agggataaac cgcgtttgat cagataatcc gagaagacac ctcccagcac gccgcccgca
     3841 aaaccacaca gtgccggaat cgaggcgacc agacccactt tcagaatcga catgcctttt
     3901 tcctgcacca gataaatcgg gaaccaggtg aggaagaacc aggtgatggt gttgataaaa
     3961 tattgtccga aaaatacgcc caacatcatg cggttagaga gcaattgctt gatgtaatgc
     4021 agtttgggac cgcttgctgc cgcgctgccc ggctttttgt ggtccatatc gaccaccgcg
     4081 ccattttcag agataaactt cagctcttcc gcagacatac gtgggtgatc tgtcgggtta
     4141 tgaatcaact tgatccacag cgccgtcagc acaaaaccaa tcacccccat aacggtgaag
     4201 acgtgctccc agccccaggc gaaagtcagc cagccaagca gcggcgaaaa gagcgccagc
     4261 gagaaatatt gcgccgagtt aaagatggcg gaggcagtac cacgttcttt cgtcgggaac
     4321 caggcggcga caatacgggc gttcgccggg aatgatggcg cttccgagaa ccccagcata
     4381 aagcgcataa agaacatgga gatccctgcc caggccagcg ggaacatatc aacaaagcct
     4441 tgcaggaagg tgaacagcga ccagaaaaag aggctgtagg tgtaaacttt tttcgagcca
     4501 aacttatcaa gcagccagcc gccgggaatt tgcatcagca ggtaggccca gccaaaagcg
     4561 gagaagatgt aacccatcga aaccgcactt aactgcaact cttttgccac ttcggtacca
     4621 gcaatagaca gcgttgcacg atcggcgtag ttaacggcgg taacaataaa aataatcagt
     4681 aatattaaat agcgggtatg cacgcctttc ttttttacgt caacggtgtc cagaatcatt
     4741 ttatttacct cgggtactta tgctgatttt tattattatg gtgaaggtgt tatttatgag
     4801 ttttatttat gccgttacga caatgaactc gggaattagt ataagcagcg cgagaataat
     4861 aatcattgtg caaatgctaa tttaattaat accatttaaa tattattttg agcatatgca
     4921 cataaggttg cgcgctaaag cacagatttg cgctttacct tgccgggcgg cactgcaatc
     4981 cctgaaatga ttgacattga tcacatttct gcgtttaaac tcctgacatt cttatttcac
     5041 ccaatgaagt catttatttt taaatgagac caggtcctca ttttaataac ccctggctgg
     5101 agaatattgc acaatggcca acatcgaaat cagacaagaa acgccaactg cgttttatat
     5161 aaaagttcac gacacagata atgtggcaat tattgttaat gataatggcc tgaaagcagg
     5221 aacgcgtttt ccggatgggc tggaattaat tgaacatatt ccccaggggc ataaagtcgc
     5281 attgctggac attccggcta atggtgaaat tattcgttat ggcgaagtga ttggttacgc
     5341 cgtgcgcgca atcccacgcg gaagctggat cgacgaatca atggttgtac taccggaagc
     5401 gccgccgtta cacacgctgc cactggcaac caaagtcccg gaacccttac cgccgctgga
     5461 aggatacacc tttgagggct atcgcaatgc cgatggcagc gtgggcacca aaaacctgct
     5521 cggcatcacc accagcgtcc actgtgtggc aggcgtggtg gactacgtag taaaaatcat
     5581 tgaacgcgat ctgctaccga aatacccgaa cgtcgatggc gtggtggggc tgaatcattt
     5641 gtacggttgt ggcgtggcga ttaacgcacc ggcggcagtt gtgcctattc gtaccattca
     5701 caatatttcg ctgaacccta actttggcgg cgaagtaatg gtgattggcc tgggttgtga
     5761 aaagttgcag cctgagcgcc tgctgactgg aacggatgat gtgcaagcta ttccagtaga
     5821 aagcgccagc attgtcagtt tgcaggatga aaagcatgtc ggttttcagt ccatggtcga
     5881 ggatattttg caggtcgccg aacgccatct acaaaaactg aatcaacgtc agcgagaaac
     5941 ctgtccggct tcagaactgg ttgtcggcat gcagtgcggt ggcagcgatg ccttttctgg
     6001 cgtaacggca aacccggcgg ttgggtatgc gtctgatcta ctggtgcgct gcggcgcaac
     6061 ggtgatgttc tcagaagtca cggaagtgcg tgacgcgatc catctgctga caccacgcgc
     6121 agtgaacgaa gaggtcggca aacggctgct ggaggagatg gagtggtacg ataactatct
     6181 caatatagga aaaaccgacc gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct
     6241 ggcaaacgtg gtagagaagg cactcggctc cattgctaaa tcgggtaaaa gcgcaattgt
     6301 tgaagtgctg tcgcccggtc aacgcccgac taaacgcgga ttaatttacg ccgcgacgcc
     6361 agccagcgat tttgtctgtg gcacgcaaca ggtggcttcg ggtatcaccg tgcaagtgtt
     6421 tacgaccggt cgtggtacgc cgtacggcct gatggcggta cccgtcatta aaatggcgac
     6481 ccgcaccgag ctggcgaacc gctggtttga tttaatggat attaacgcgg gcactatcgc
     6541 caccggcgaa gaaaccattg aagaggtggg ctggaagttg ttccacttta ttctcgacgt
     6601 cgccagcggg aagaagaaaa ccttctcgga tcaatggggg ctgcataacc agctggcagt
     6661 gtttaacccg gcaccggtga cctgatttct tttggaacgt ccctcgcaaa acatggcctt
     6721 agtgccatgt ttttattgtt taaagccccc acgtccatta ataatgcatt tgcattacct
     6781 tagttcaagc ttataatttg agcagaaaac aggctgtaaa aggacagtga atcatgcccg
     6841 ctaatgctcg ctctcacgct gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa
     6901 caactatccc cgcgccagtg cgtgaggcct taaaactgaa gccaggccag gacagcattc
     6961 attacgaaat tctgcctggt gggcaagtct ttatgtgccg actgggagat gaacaggagg
     7021 atcatactat gaatgcattt ttgcgttttc tggatgcaga tatccagaac aacccgcaaa
     7081 aaactcgtcc attcaacatt caacaaggaa agaaacttgt cgctggcatg gacgtcaaca
     7141 ttgatgatga gattggcgac gacgaataat ggattttcca caaagggtta atggttgggc
     7201 gctatatgct catccctgtt ttcaggaaac ctacgatgct ttagttgccg aagtcgaggc
     7261 attaaaggga aaagatcctg aaaattatca gagaaaagct gccacaaagt tattggcggt
     7321 ggtccataaa gtgattgagg agcatatcac ggtcaatcca tcatcaccgg cattccgtca
     7381 tggcaagtcg ttaggctctg ggaaaaataa agactggtca cgggtaaaat ttggtgctgg
     7441 tcgctatcgt ctcttctttc gttacagtga aaaagagaaa gtcatcattc tgggatggat
     7501 gaacgatgaa aacacgctgc gcacctacgg taaaaaaaca gatgcctata ccgtattcag
     7561 taaaatgtta aaaagaggac atcctcctgc cgactgggaa agcctcaccc aagagacaga
     7621 agaaaaccat tgatggtgtt cacaatgccc gccgaacgta ttcgtgttga ccagcgggca
     7681 ccctttctca ctccccgacc agaatcactt caaccccagc ctttcgcagt ccttccagac
     7741 tatccgcagg aatgccttca tcaacaatga tcatgtcgat acgttgagta tcaatgatct
     7801 tatgtaaact ggaacgattg aacttactgg aatcggtgac tacgatgatc cgttccgcaa
     7861 cttcgcacat ccggcggttt aaacgggctt catcttcatt atgcgtgctg acgccgcgct
     7921 ccagatcgat cgcatctaca ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt
     7981 gttcagcctg atcgccgtaa aaagattgcg actgacggcg caaatgcccg ccggtcatca
     8041 gcagctcaac gccttccgct tccagcaaag cattagccac gttcataccg ttggtcatcg
     8101 caattacgtc agtgtgcttg cgcatcagac gagcaatctc aaaagtggta gtcccggaat
     8161 cgaggatcac ccgatgacct ggctgaatca actcaacggc agctttcgca acgctgcgtt
     8221 tcatcgcggt gttcagtgcg cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc
     8281 tatcgcagat caacgcgcca ccataggcac gcacagcgat cccctgcttt tccagaaacg
     8341 ccagatcgtt gcggatcgtc acggtagata cgccatacaa tgccgacaga tcgttaacct
     8401 gcacactccc ttgctgtcgc agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg
     8461 tcactcgctt ctcacctgaa gcgtcggtat tactcatagt aagtcctttc gtaaaacttt
     8521 cgtttcattt cgttttgcct attaacgcct ttctattaag caaatgcaag cccaccttgc
     8581 ccattgacgc aagctactct cgtttcagtg actttcatta tgtttctttt gtgaatcaga
     8641 tcagaaaact attatctttc gttttatttt tatctcacca tgacgcagta tcaactgaaa
     8701 caaaacgaaa gattaatatc gcagtaatct gaactggaga ggaaagtgaa acatctaaca
     8761 gaaatggtga gacagcacaa agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca
     8821 catccgctgg tgctggaagc tgcaatccgc tacgccagtg caaaccaaac gccgttactg
     8881 attgaagcaa cctccaatca ggtagaccag ttcggcggtt ataccggaat gacgcccgcc
     8941 gattttcgcg gctttgtttg tcagctcgcc gactcgttga atttcccgca ggatgcgttg
     9001 attctgggtg gtgaccatct ggggccaaac cgctggcaaa acctgccagc cgctcaggca
     9061 atggccaatg ccgatgattt gattaaaagc tacgttgcgg caggattcaa aaaaatccac
     9121 cttgattgca gcatgtcctg tcaggacgat ccaattccct taactgatga catcgtggct
     9181 gaacgcgccg cccgtctggc gaaagtggcg gaagaaacct gtcttgaaca ctttggcgaa
     9241 gccgatctgg agtatgtcat tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc
     9301 ttaagcgagc tggcggtcac cacgccggat gccgcccgcg ccacgctgga agcccatcgt
     9361 cacgcctttg aaaagcaagg tttgaatgcc atctggccac gcatcattgc cctggtggtt
     9421 caacccggcg tcgaattcga tcacaccaac gttattgatt atcagcccgc caaagcgagc
     9481 gccttaagcc agatggtcga aaactacgaa acgctgattt tcgaagcgca ctctaccgat
     9541 tatcaaacgc cgcaatcgct gcgccagctg gtgattgacc actttgccat tctgaaagtt
     9601 ggcccagcgc tgaccttcgc cctgcgtgaa gctctgttct ctctggcggc gattgaagaa
     9661 gaactggtgc cagcgaaagc ctgttctggt ctgcgtcagg tgctggaaga cgtgatgctc
     9721 gaccgcccgg aatactggca aagccactac cacggtgacg gcaacgcgcg tcgtctggcg
     9781 cgtggttata gctactcgga tcgcgtgcgc tattactggc cggacagcca gattgatgac
     9841 gctttcgctc atctggtacg taatctggcg gattcaccaa ttccgctgcc gctgatcagc
     9901 cagtatctgc cgctgcagta cgtgaaagtt cgctccggcg agctgcagcc aacgccacgg
     9961 gaactcatta tcaaccatat tcaggacatc ctggcgcagt accacacagc ctgtgaaggc
    10021 caataagcaa aacaaagagg aacacgctat gccaaatatt gttttaagcc ggattgatga
    10081 acgcttgatt cacggtcagg tcggcgttca atgggtcgga tttgcggggg caaatctggt
    10141 gctggtagcc aacgatgagg ttgccgaaga tccggtacaa caaaacctga tggaaatggt
    10201 actggcagaa gggatcgccg tacgtttctg gacgctgcaa aaagttatcg acaacattca
    10261 tcgcgccgcc gatcgacaga aaatcctgct ggtttgtaaa acacccgccg atttcctgac
    10321 gctggtgaaa ggtggcgttc cggtgaatcg cattaacgtt ggcaatatgc actacgccaa
    10381 tggcaaacaa caaatcgcca aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa
    10441 cgacctgaaa gccgctgggg tggaatgctt cgttcagggc gtcccgacag agcctgctgt
    10501 ggacctcttt aaattacttt gagggattca tcatggaaat cagcctgttg caggcatttg
    10561 cgttgggcat tatcgccttt atcgctggcc tggatatgtt taacggccta acacatatgc
    10621 accgcccggt ggtcctcggc ccgttggtcg ggctggtact tggcgatctg cataccggaa
    10681 ttttaaccgg cggtacgctg gaactggtgt ggatggggct ggccccgctg gcgggcgcac
    10741 agccgcctaa cgtgattatc ggtactatcg tcggcacggc gtttgccatt actactggcg
    10801 tgaaacccga tgtcgcagtc ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga
    10861 ttaccttcct gttctcggtg atgtccggcg tgatgtctcg ctgcgaccgg atggcggaga
    10921 acgccgatac tcgcggcatt gaacgcgtga actatctggc actgctggca ctcggcactt
    10981 tctattttct ctgcgctttc ctgcctatct acttcggcgc ggaacatgca aaaaccatca
    11041 ttgatgtcct gccgcaacga ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag
    11101 caatcggttt tgccgtgctg ctgaaaatca tgatgaaaaa cgtctacatc ccctacttca
    11161 tcctgggctt tgttgccgca gcctggctca agttaccggt gctggctatt gctgccgctg
    11221 cgctggcgat ggcgctgatc gacctgctgc gtaaatctcc tgaaccgact caacctgcgg
    11281 cacagaaaga ggaatttgaa gatggcatct aatcaaacca ccctgccgaa cgtcagtgaa
    11341 aacgaagaaa cattgctgac tggcgtcaat gaaaacgtgt atgaagatca gagcattggc
    11401 gcggagctga cgaaaaaaga tatcaaccgc gtcgcctggc gttccatgct gttacaagcg
    11461 tcttttaact acgaacgtat gcaggcttct ggctggttgt acggtctgct gcctgctctg
    11521 aaaaagatcc acactaataa acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc
    11581 ttcaataccc atccgtttct ggtgacattt gttatcggca ttatccttgc gatggagcgt
    11641 tctaagcagg acgttaacag tattcagagc accaaaattg ccgtcggtgc gccgctcggc
    11701 gggattggcg atgcaatgtt ctggctaacg ctactgccga tttgtggcgg gattggagcc
    11761 agtctggcat tgcaaggctc cattcttggc gcagtcgtct ttattgtgct gttcaacgtg
    11821 gtacacctgg ggctgcgttt tggtctggcg cattatgctt accgcatggg cgtggcggcg
    11881 attccactca ttaaagcgaa taccaaaaaa gtcggccatg ctgcgtcaat cgttgggatg
    11941 acggtaatcg gcgcgctggt ggcaacctat gttcgtttga gcaccacgtt ggaaatcaca
    12001 gcgggcgacg cagtggttaa gttacaggct gatgttatcg acaaactgat gcctgccttc
    12061 ttaccgttgg tctacaccct gaccatgttt tggttggtac gccgcggctg gagtccgctg
    12121 cgtctgattg cagttaccgt ggttctcggc atcgtcggta aattctgcca tttcctttaa
    12181 atacaatgag gtattagatg ttaagtatta ttttgacagg gcatggcgga tttgccagcg
    12241 gcatggaaaa agcaatgaag caaatcctcg gcgagcaatc gcagtttatc gccatcgatt
    12301 ttccggaaac ctcaagcacc gcgctgctta catcgcagct tgaagaggcc atcgcccaac
    12361 tggattgtga agacggcatt gtttttctga cggatttgct gggcggcacg ccgtttcgcg
    12421 tagcttcaac gctggcgatg caaaaaccgg gctgtgaagt gatcaccggc accaatttgc
    12481 aactgctact ggagatggtg ctggagcgcg aagggttaag cggtgaagag ttccgtgtgc
    12541 aggcgctgga gtgtggacac cgtgggctga ccagtctggt agacgagtta ggccgctgcc
    12601 atgaagaatg tccggtcgag gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct
    12661 gactgaagag ggatggctcg atgaccatca gttgtgtatt gctgacggtg taatcgcagc
    12721 aatcgaaccg attccagtgg gcgtgactga acgcgatgcg gaactgctct gccccgctta
    12781 catcgacacc catgtacacg gtggtgcggg cgttgatgta atggatgacg cgccggatgt
    12841 actcgacaag ctggcaatgc acaaggcacg cgaaggtgtc ggcagttggt tgccgactac
    12901 cgtaaccgcg ccgcttaaca ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca
    12961 acgcggcgga cctggtgcgc aagtgctggg gagttatctc gaaggaccgt acttcacgcc
    13021 gcagaataaa ggcgcgcatc cgccggagct gttccgcgag ctggaaattg ccgagctgga
    13081 tcagttgatt gccgtttctc agcacacctt acgtgtggta gcgctggcac cggaaaaaga
    13141 aggggcattg caggccatcc gccatcttaa acagcaaaac gtaagggtga tgctggggca
    13201 tagcgcggcg acctggcaac aaacccgcgc cgcatttgat gctggtgccg acggcctggt
    13261 gcattgctat aacgggatga caggtttaca tcaccgcgaa ccgggaatgg ttggcgcggg
    13321 attaacggac aagcgcgcct ggctggaact gatagccgat ggtcatcatg tgcatccggc
    13381 ggcgatgtcg ctgtgttgtt gctgtgcgaa agagagaatc gtgctgatca ccgacgcgat
    13441 gcaggcagcc gggatgccgg atggtcgcta tacgttatgt ggtgaaaaag tgcagatgca
    13501 cggtggcgtt gtccgtaccg cgtctggtgg gctggcgggc agtacgctgt ctgttgatgc
    13561 ggcagtgcgc aacatggtcg agttgacggg cgtaacgcct gcggaagcca ttcatatggc
    13621 atcgctgcat ccggcgcgaa tgctgggtgt tgatggtgtt ctgggatcgc ttaaaccggg
    13681 caaacgcgcc agcatcgttg cgctggatag cgggctgcat gtgcaacaaa tctggattca
    13741 gagtcaatta gcttcgtttt gatagtttgc tcctttattg ggccttcact tcccccgtaa
    13801 ggcctttctt tttctttcgt tttgatctgt gcagcggtgt cggatgcgac gctaacgcgt
    13861 cttatgcgac ctacggtacg ccgcatccag caatcggtga acatagcgac gtcattacgc
    13921 aattctttaa tcttcccttg tcttttcttt caatgatcac agatttcgtt ttatttcctt
    13981 tttctccatt gaactttcgg tttcttttct atagatttta atcaacgaaa gacatcacca
    14041 agtgaaatga aacgaaaggc aagtgaaagc gacaacgccc gacgtcaagt tcatcagact
    14101 aaggattgag ttatgccaaa aaattacacc cctgctgccg ccgcaaccgg tacatggact
    14161 gaagaagaga tccgccatca gcctcgcgca tggatccgtt cactcaccaa catcgacgcg
    14221 ctacgttccg cgctcaataa cttccttgaa ccgttactgc gcaaagagaa tctgcggatc
    14281 atcctgaccg gagccggaac ctcggcattt atcggtgaca tcatcgcgcc gtggctcgcc
    14341 agccataccg gtaaaaactt cagcgccgta ccgaccaccg atctggtcac taatccgatg
    14401 gactacctga acccagccca tccgctgctg ttgatctcct tcggtcgatc cggcaacagc
    14461 ccggaaagcg tcgcagccgt ggaactggca aatcaatttg taccggaatg ctatcacctg
    14521 ccgatcacct gcaacgaagc gggcgctctt taccaaaacg cgatcaacag cgataacgcg
    14581 tttgccctgc tgatgcccgc agaaacgcac gatcgcggct ttgcgatgac cagcagcatt
    14641 accaccatga tggccagctg cctcgcggtt ttcgcacctg agacgatcaa cagccaaacc
    14701 ttccgcgacg tggcggatcg ttgccaggcg atcctgacct cactgggcga tttcagcgaa
    14761 ggtgtgtttg gttacgcacc gtggaaacgg atcgtttatc tcggtagcgg tggcttacag
    14821 ggcgcagcac gcgagtcggc gctgaaagtg ctggaactga cggcgggtaa actggcggcc
    14881 ttttatgatt ctccaaccgg attccgtcat ggaccaaaat cgctggtcga tgacgaaacg
    14941 ctggtggtgg tatttgtctc cagccaccct tacacccgtc agtatgatct tgatctgctg
    15001 gctgaacttc gccgtgacaa ccaggcaatg cgtgtaatcg ccatcgccgc ggaaagcagc
    15061 gacatcgtcg ctgccagtcc acatatcatc ctgccaccgt cacgtcactt tatcgacgtt
    15121 gagcaggcat tttgcttcct gatgtacgcc cagacgtttg cactgatgca gtcgctgcac
    15181 atgggcaata cgccggatac cccatcagcc agtggcaccg ttaaccgcgt ggtgcaaggc
    15241 gtaatcattc atccgtggca ggcataagag gatcgcatta tgagcattat ctccactaaa
    15301 tatctgttac aggacgccca ggccaatggc tacgcggtgc ctgcttttaa cattcataac
    15361 gccgagacga tccaagcgat cctcgaagtg tgcagtgaaa tgcgatcgcc ggtgatcctc
    15421 gccggaacgc cggggacctt taaacacatc gcgctggaag agatctacgc cctgtgtagc
    15481 gcctattcca caacctacaa catgccactg gcgctgcatc tcgaccacca cgaatcgctg
    15541 gatgatattc gccgtaaagt ccacgcaggc gtgcgcagtg cgatgatcga cggcagccac
    15601 ttcccgtttg ccgagaacgt aaagctggtg aaatcggttg tcgacttctg ccactcccag
    15661 gattgcagcg tggaagcaga actgggccgc ctgggcggtg ttgaagatga catgagcgtt
    15721 gacgccgaaa gtgcattcct gaccgatcca caagaagcta aacgctttgt cgaactgact
    15781 ggcgtcgaca gcctggcggt agcgattggt acggcgcacg gcttatacag caaaacgccg
    15841 aagattgatt tccagcggct ggcggaaatt cgtgaagtgg tggatgttcc tctggtgctg
    15901 catggtgcca gcgatgttcc ggatgaattt gtccgtcgca ctattgaact tggcgtcaca
    15961 aaagtgaacg ttgccacaga attaaaaata gccttcgctg gcgcggttaa agcctggttt
    16021 gcggaaaatc cgcagggtaa tgatcctcgt tattatatgc gcgtcggaat ggatgcgatg
    16081 aaagaagttg tcagaaataa aattaatgtc tgtggttcag cgaatcgaat ttcagcataa
    16141 tcattagttt tttatgattt atcccaatgt acttcccgat taatatcggg gagtgcctta
    16201 atggaaaagg agataactaa accttaataa atacatcagt acaatatcgc aacaataata
    16261 tatttaaaaa aattatatta ttcaacttta tggtgaggat tacacaatga gcagtccaaa
    16321 tattctctta acccgtattg ataaccgtct ggttcatggc caggttggcg tgacctggac
    16381 atccaccatc ggtgcaaatc tgctggtagt cgtggatgat gttgtcgcta acgatgatat
    16441 tcaacagaaa ttaatgggta ttaccgcgga aacctacggc tttggcattc gtttctttac
    16501 tatcgaaaaa accattaacg tcatcggcaa agctgcacca catcagaaga tcttcctgat
    16561 ttgccgtacg ccacaaacgg tacgtaaatt ggtagaaggt ggcattgacc tgaaagatgt
    16621 caacgtcggc aatatgcatt tctcggaagg gaaaaagcaa atcagcagta aagtttatgt
    16681 cgatgaccag gatctcacgg acttacgttt tattaaacaa cgtggcgtga atgttttcat
    16741 tcaggatgtc cctggcgatc aaaaagaaca aatccctgac taaatctaaa atcgccttaa
    16801 tattggtttg aggtaataaa aatgcatgaa ataaccctac ttcagggatt atccctggcg
    16861 gcgttagttt tcgttctggg gattgatttt tggctggaag ccttattttt attccgcccg
    16921 ataatcgttt gtacccttac aggtgctatt ctcggtgata ttcagactgg cttaattacc
    16981 ggtggtctga cagagttggc tttcgccgga ttaacccctg caggtggtgt tcagccgccc
    17041 aacccgatta tggcgggtct gatgaccacc gtcattgcat ggtctacggg cgttgatgcc
    17101 aaaacagcaa ttggtcttgg cctgccgttt agtttgttaa tgcagtacgt cattctgttc
    17161 ttctattccg ctttctcatt atttatgacc aaagccgata aatgcgcgaa agaggcggat
    17221 acggcagcgt tttcccggct taactggaca acgatgctca tcgtcgcttc agcgtatgcg
    17281 gtgattgctt tcctctgtac ttacctggca caaggggcga tgcaggcgct ggtgaaagcg
    17341 atgcccgcct ggctgaccca cggctttgaa gtggctggcg gtattctgcc tgccgttggt
    17401 tttggcttgc tgctgcgcgt gatgttcaaa gcacaatata tcccttacct gatcgccggc
    17461 ttcctgtttg tttgctacat ccaggtcagt aacctgttgc cggttgccgt actgggcgca
    17521 ggctttgcgg tgtatgagtt tttcaatgcg aaatcccggc agcaagcgca accgcagccc
    17581 gttgccagta aaaatgaaga agaggactac agcaatggga tctgaaatca gtaaaaaaga
    17641 tatcacccgt ctgggctttc gttcgtcact gctgcaagcg agctttaact acgaaaggat
    17701 gcaggcgggc ggttttacct gggcgatgtt gccgatcctg aaaaagattt ataaggatga
    17761 caaaccgggc ctaagcgcag cgatgaaaga taacctcgaa tttattaata cccacccgaa
    17821 tctggtcgga ttcctgatgg ggttattaat ttcgatggaa gaaaaaggag aaaaccgcga
    17881 caccattaaa ggcctcaaag tggcactgtt tggcccaatc gccgggattg gcgatgcgat
    17941 tttctggttt actttgttgc cgattatggc gggaatttgc tcatcatttg ccagccaggg
    18001 aaacctgctg gggccgattc tatttttcgc cgtttacctg cttatctttt tcctgcgcgt
    18061 cggctggacc cacgtcggtt attcagtcgg cgtgaaggcg atcgataaag tacgagagaa
    18121 ctcgcagatg attgcccgtt cggcaaccat cctcgggatc acggtaatcg gcgggctgat
    18181 cgcttcgtat gtgcatatta acgtggtgac atcgtttgcc atcgacagta cccacagcgt
    18241 cgcgctgcag caggatttct tcgataaagt cttcccgaac attttaccga tggcctacac
    18301 cctgctgatg tattacttcc tgcgggtgaa aaaagcgcat ccggtgctgt taatcggcgt
    18361 gacttttgtg ctctctattg tttgttccgc attcggcatt ttgtaaatgg aacgaggcac
    18421 tgcgtctggt ggtgcctctt tacttaagga atttcatcct gtgcaaaccc ttcagcaagt
    18481 tgaaaactat acggcgttaa gtgaacgtgc cagcgaatat ttattggctg tgatccgtag
    18541 caaaccggat gccgtgattt gcctggcgac tggagccacg ccattactga cgtatcatta
    18601 tctggtagaa aaaatccatc agcagcaggt tgatgtcagc caactcacct tcgtgaagct
    18661 cgacgaatgg gtggatctgc cattaacgat gcctggcacc tgcgaaactt tcctgcaaca
    18721 gcatatcgtg cagccgctgg ggctacgtga agaccagctc atcagctttc gctccgaaga
    18781 gataaatgag acagagtgcg aacgggtaac gaacctgatt gcgcgcaaag gcggtctgga
    18841 tttatgcgtt ctcggattgg ggaaaaacgg tcatcttggg ctgaacgaac cgggagaaag
    18901 cctgcaaccg gcctgccata tcagtcaact tgatgccaga acacagcaac atgagatgtt
    18961 aaaaaccgcg ggtcgccccg tgactcgtgg gatcacctta ggcctgaagg atattctcaa
    19021 tgcccgcgaa gttttgttac tggtgactgg cgaaggaaag caggatgcga cagaacgttt
    19081 tctcacggct aaagtctcca ccgctatccc ggcttcgttt ttatggctgc acagtaactt
    19141 tatttgttta attaacacct aaccagatga aaaatttgct caaaatgttt aaattaactt
    19201 a
//
LOCUS       DAFNBJ010000091        19047 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000091 DAFNBJ010000000
VERSION     DAFNBJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.091"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            78..1133
                     /gene="phoE"
                     /locus_tag="L2X49_003042"
     CDS             78..1133
                     /gene="phoE"
                     /locus_tag="L2X49_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBN4798091.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1172..1573)
                     /gene="crl"
                     /locus_tag="L2X49_003043"
     CDS             complement(1172..1573)
                     /gene="crl"
                     /locus_tag="L2X49_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBN4798092.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1631..2875)
                     /gene="frsA"
                     /locus_tag="L2X49_003044"
     CDS             complement(1631..2875)
                     /gene="frsA"
                     /locus_tag="L2X49_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBN4798093.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2967..3425)
                     /gene="gpt"
                     /locus_tag="L2X49_003045"
     CDS             complement(2967..3425)
                     /gene="gpt"
                     /locus_tag="L2X49_003045"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBN4798094.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDNYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3686..5143
                     /gene="pepD"
                     /locus_tag="L2X49_003046"
     CDS             3686..5143
                     /gene="pepD"
                     /locus_tag="L2X49_003046"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBN4798095.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            complement(5200..5552)
                     /gene="prfH"
                     /locus_tag="L2X49_003047"
                     /pseudo
     CDS             complement(5200..5552)
                     /gene="prfH"
                     /locus_tag="L2X49_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5548..5754)
                     /locus_tag="L2X49_003048"
                     /pseudo
     CDS             complement(5548..5754)
                     /locus_tag="L2X49_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(6073..6525)
                     /locus_tag="L2X49_003049"
     CDS             complement(6073..6525)
                     /locus_tag="L2X49_003049"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN4798096.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIERHIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQIVKQQHVECRGAWFTNFYMRYKPQH"
     gene            complement(6522..7577)
                     /gene="dinB"
                     /locus_tag="L2X49_003050"
     CDS             complement(6522..7577)
                     /gene="dinB"
                     /locus_tag="L2X49_003050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBN4798097.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELHLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(7648..8433)
                     /gene="lafU"
                     /locus_tag="L2X49_003051"
     CDS             complement(7648..8433)
                     /gene="lafU"
                     /locus_tag="L2X49_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBN4798098.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFECGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            8405..10117
                     /locus_tag="L2X49_003052"
                     /pseudo
     CDS             8405..10117
                     /locus_tag="L2X49_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(10435..10932)
                     /gene="rayT"
                     /locus_tag="L2X49_003053"
     CDS             complement(10435..10932)
                     /gene="rayT"
                     /locus_tag="L2X49_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBN4798099.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRHAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(11108..11857)
                     /locus_tag="L2X49_003054"
     CDS             complement(11108..11857)
                     /locus_tag="L2X49_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBN4798100.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GENIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            12158..12898
                     /locus_tag="L2X49_003055"
     CDS             12158..12898
                     /locus_tag="L2X49_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBN4798101.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(12869..13636)
                     /locus_tag="L2X49_003056"
     CDS             complement(12869..13636)
                     /locus_tag="L2X49_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBN4798102.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13842..14420)
                     /gene="lpcA"
                     /locus_tag="L2X49_003057"
     CDS             complement(13842..14420)
                     /gene="lpcA"
                     /locus_tag="L2X49_003057"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBN4798103.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            14660..17104
                     /gene="fadE"
                     /locus_tag="L2X49_003058"
     CDS             14660..17104
                     /gene="fadE"
                     /locus_tag="L2X49_003058"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HBN4798104.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLCSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(17147..17593)
                     /gene="ivy"
                     /locus_tag="L2X49_003059"
     CDS             complement(17147..17593)
                     /gene="ivy"
                     /locus_tag="L2X49_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBN4798105.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            17774..18544
                     /gene="yafV"
                     /locus_tag="L2X49_003060"
     CDS             17774..18544
                     /gene="yafV"
                     /locus_tag="L2X49_003060"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBN4798106.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQNDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWQDADEFRLR"
     gene            complement(18586..>19047)
                     /locus_tag="L2X49_003061"
     CDS             complement(18586..>19047)
                     /locus_tag="L2X49_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HBN4798107.1"
                     /translation="SEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSI
                     IAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRG
                     NAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         4577 a         4890 c         4964 g         4616 t
ORIGIN      
        1 tcaggttaaa aactttcctg ttttcaacgg ggctctcccg ctgaatattc gcgcgttaat
       61 taaaatcagg aatgaaaatg aaaaagagca ctctggcatt agtggtgatg ggcattgtgg
      121 catctgcatc cgtacaggcc gcagaaatat ataacaaaga cggtaataaa ctggatgtct
      181 atggcaaagt taaagccatg cattatatga gtgataacga cagtaaagat ggcgaccaga
      241 gttatatccg ttttggtttt aaaggcgaaa cacaaattaa cgatcaactg actggctatg
      301 gccgttggga agcggagttt gccggaaata aagcggagag tgatactgca cagcaaaaaa
      361 cgcgtctcgc ttttgccgga ttgaagtata aagatttggg ttctttcgac tatggccgta
      421 acctgggcgc gttgtatgac gtggaagcct ggaccgatat gttcccggaa tttggtggcg
      481 actcctcggc gcagaccgac aactttatga ccaaacgcgc cagcggtctg gcgacgtatc
      541 ggaacaccga cttcttcggc gttatcgatg gcctgaactt aaccctgcaa tatcaaggga
      601 aaaacgaaaa ccgcgacgtt aaaaagcaaa acggcgatgg cttcggcacg tcattgacat
      661 atgactttgg cggcagcgat ttcgccatta gtggtgccta taccaactca gatcgcacca
      721 acgagcagaa cctgcaaagc cgtggcactg gcaagcgtgc agaagcatgg gcaacaggtc
      781 tgaaatacga tgccaataat atttatctgg caacttttta ttctgaaaca cgcaaaatga
      841 cgccaataac tggcggcttt gccaataaga cacagaactt tgaagcggtc gctcaatacc
      901 agtttgactt tggtctgcgt ccatcgctgg gttatgtctt atcgaaaggg aaagatattg
      961 aaggtatcgg tgatgaagat ctggtcaatt atatcgatgt cggggctaca tattatttca
     1021 acaaaaatat gtcagcgttt gttgattata aaatcaacca actggatagc gataacaaat
     1081 tgaatattaa taatgatgat attgtcgcgg ttggcatgac ctatcagttt taatgattat
     1141 tgccggatgt gatgcatccg gcacatttca ctcacgccgt taacttcacc ggctcgtcac
     1201 gaaaatcatc cgccggttcc agcttcagat tcagcgtcgt cagcagttca cgcagcttct
     1261 cgtgaaactc acgcagggtg tgctccagtc gttcaaccac ttcagtgtct tttaccggaa
     1321 cgctcttcca gtcgcctgct ttatcgaaca gaccaaactg gtagctgtag gtaaaacggg
     1381 attcctgcgc ttccagctcc atccaccagc cccagaattc acgcacttcc ggtgccggtt
     1441 tcacgttgac gcatacagcc agacaatcga aaaagaatcg attatcttcg cacttacctt
     1501 cacgaatata cgggcctagt gcggtaaatt ttttgatcaa tctgctcttc gggtgtccac
     1561 tcggtaacgt cattgctatc tcctgttgtg atgcaactgt tttaccaaat tggcaaaatt
     1621 tagcaaattt ttaacacaag cgtttttcga tccagccggt gatttcctga agacctttgt
     1681 caaaattccg atacaccggg ttaaatggga tctctaataa tttaccgtca gcagatgatg
     1741 aggtgattaa gcgtgagtcc tcttccgggc tgaacggatc gttcttccag tagcctgata
     1801 acattggcgt tgggcagcga cgtcccagca atccttgcac ttttaatgaa tagcgattca
     1861 gctccacgcg caacgcgtca tcggaagcat catgcatccc caaacgactc gccagaacgt
     1921 caagatacat ttccggcacc tgttgctggc acttaaaatc actcaacagg gtatgaacta
     1981 ccggaccaag acaggcaacc gctttcagac gcggcgattc aaggtatgcc agacgcacgg
     2041 caacgttagc gccgaaacgg aaaccaaagg ccgcgacgcg agtgtgatcc acccacggta
     2101 cgttaggcag cgcctttaag acgtgctgat gcaacaggct ggagtcctgg gtgagcttcc
     2161 attttgaaga aaagcccacc gacggcatat caatagtcag catcgcaatg ccgcgcggcg
     2221 caaaataacg ttcatacagg ctgtaatagt ccgtctgcat cgcatccaga ccaccacaca
     2281 ttaatactgt cgggaacggg ccatcgcctt tcggcatatg caaaaagccg gtgatgggcg
     2341 caccgccggg tacggtaaac tccatctgcc gcatcgtgcc cggcagacgc tgggcggcct
     2401 cttcataggc gcggtttgat aaagcctgcg cttgctcggc caggtcatct cctttcagat
     2461 gaggataggc ggcaatgttg tacaacgtag ccgcatgcag ccagtgtcga ccactgagtt
     2521 gcggatcttc ttccgcacag gctttttgtt gccacaccat cgcctgggtg gcccactcat
     2581 aaatccagtt gccaccacgg tagccaatca cggtatcgta taaatcatca tcggtacgtt
     2641 cagcatcgct catcacaata cgtgcctgga cgtcgaggat ttcacgtggg tcaatgccgc
     2701 gccagatcca catcagacgg ttaatcatgc gataccagtg agggatggtt ttaccatcaa
     2761 gggccgactg cacaggcggt tgtgcgccgt gattaaagcg gcggactagc gtcgaggttt
     2821 caggatgttt aaagcggggt ttgaacaggg tttcgctcag gtttgcctgt gtcatggatg
     2881 cagcctccag aatacttact ggaaactatt gtaacccgcc tgaagttaaa aagaacaacg
     2941 cccggcagtg ccaggcgttg aaaagattag cgaccggaga ttggcgggac gaatacgacg
     3001 cccatatccc acggctgttc gatccaggta tcttgcggga tatcaacaac ataattatca
     3061 accagcggac gaccagccgg ttttgcaaag atggtgacaa agtgcgcttt tggatacatt
     3121 tcacgaatcg caaccgcagt accaccggta tccaccaggt catcaataac gatgaagcct
     3181 tcgccatcgc cttctgcgcg tttcagcact ttaagctcgc gctggttgtc gtgatcgtag
     3241 ctggaaatac aaacggtatc gacatgacga atacccagtt cacgcgccag taacgcaccc
     3301 ggtaccagac cgccacggct tacggcaata atgcctttcc attgttcaga aggcatcagt
     3361 cggcttgcga gtttacgtgc atggatctgc aacatgtccc aggtgacgat gtatttttcg
     3421 ctcatgtgaa gtgtcccagc ctgtttatct acggcttaaa aagtgttcga ggggaaaata
     3481 ggttgcgcga gattatagag atctggcgca ctaaaaacca gtatttcaca tgagtccgcg
     3541 tctttttacg cactgcctct ccctgacgcg ggataaagtg gtattctcaa acatatctcg
     3601 caagcctgtc ttgtgttgac aacattttct gctaaccctg tgacctgcaa tactgttttg
     3661 cgggtgatcg acaaggagac ttaacgtgtc tgaactgtct caattatctc cacagccgct
     3721 gtgggatatt tttgccaaaa tctgttctat tcctcacccg tcctatcatg aagagcaact
     3781 cgctgaatac attgttggtt gggcaaaaga gaaaggtttc catgtcgaac gcgatcaggt
     3841 aggtaatatc ctgattcgta aacctgctac cgcaggtatg gaaaatcgta aaccggtcgt
     3901 cttgcaggcc cacctcgata tggtgccgca gaaaaataac gacaccgtgc atgacttcac
     3961 gaaagatcct atccagcctt atattgatgg cgaatgggtt aaagcgcgcg gcaccacgct
     4021 gggtgcagat aacggcattg gtatggcgtc tgcgctggcg gttctggctg acgaaaacgt
     4081 ggttcacggc ccgctggaag tgctgctgac catgaccgaa gaagccggta tggacggtgc
     4141 gttcggctta cagagcaact ggttgcaggc tgatattctg attaacaccg actccgaaga
     4201 agaaggtgaa atctacatgg gttgcgcggg gggtatcgac ttcacctcca acctgcattt
     4261 ggatcgtgaa gcggttccag ctggctttga aaccttcaag ttaaccttaa aaggtctaaa
     4321 aggcggtcac tccggcggtg aaatccacgt tggcctgggt aatgccaaca aactgctggt
     4381 gcgcttcctg gcgggtcatg cggaagagct ggacctgcgc cttatcgatt tcaacggtgg
     4441 cacactgcgt aacgccatcc cgcgtgaagc ctttgcgacc attgctgtcg cagctgataa
     4501 agtcgacgcc ctgaaatctc tggtgaatac ctatcaggac atcctgaaaa acgagctggc
     4561 agagaaagag aagaatctgg ccttgttgct ggactctgta gcgaacgata aagctgctct
     4621 gattgcgaaa tctcgcgata cctttattcg tctgctgaac gccaccccga acggtgtgat
     4681 ccgcaattcc gacgtggcaa aaggtgtggt cgaaacctcc ctgaacgtcg gtgtggtgac
     4741 catgactgac aataacgtag aaattcactg cctgatccgt tcactgatcg acagcggtaa
     4801 agactacgtg gtgagcatgc tggattcgct gggtaaactg gctggcgcga aaaccgaagc
     4861 gaaaggcgca tatcctggct ggcagccgga cgctaattct ccggtgatgc atctggtacg
     4921 tgaaacctat cagcgcctgt tcaacaagac gccgaacatc cagattatcc acgcgggcct
     4981 ggaatgtggt ctgtttaaaa aaccgtatcc ggaaatggac atggtttcta tcgggccaac
     5041 tatcaccggt ccacactctc cggatgagca agttcacatc aaaagcgtag gtcattactg
     5101 gacactgctg actgaactgc tgaaagaaat tccggcgaag taattatttg atttgctgcc
     5161 ggatggcgtt taatcgcctt ccggcagttt catccttcat tatccttcga taaaagccat
     5221 ccctgtaaat gtccgtcgcg ggttgccacg ttcaatctgg tgatggaaca ttcgccgctg
     5281 cgatttcagc gccgcgctat tttcctgttg ctgctgctcc agcttccagg caatcagcaa
     5341 tcgcgccagc cgtttgttag catgcccaga aaccagtttt tgcgcccatg atgagaccga
     5401 tacggactcg gacaaatcca ctgaatagtg ccgcaccagc tttcgcttaa tgcccatgcg
     5461 ttatcgccat ccagagaaac cagcgccgaa cgcagcgtgt cagagtagcg gcccgtttct
     5521 gtttccagca ccgttaccgc gacgtcttgc cggataaaaa cgaccaacag agaagaacgc
     5581 tgcgccaatc ggatagccgc gtccgggatg taaatctggc atccccacta cgcgctgcat
     5641 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     5701 atcagatgcg atggtaaata ccgcatcgga taagggacga atatatttgc ccatgtacca
     5761 ttccatttat actcgtcata cttcaagttg catgtactgc gactgcgttc gttcacccca
     5821 gtcacttact ttagtaagct cctggcgatt cactcgcttg tcaccttcct gcaactcgaa
     5881 ttatttagag tatataaata ttaaaaatca ggaaatggca gacgaatcca gggcaagaaa
     5941 aaaagaatta atcctggaaa ggtctgcaaa gagtcattga taaaggattt gtcatgattt
     6001 acctccttgc agtttgtgga ataagaaaac gcgcacatac caggcgggcg ttattttcat
     6061 tgcaagctgg atttaatgtt gcggtttata tcgcatataa aaattagtaa accacgctcc
     6121 ccggcattca acgtgctgct gcttaactat ctgaaaacca taacgttcaa aaaagggttt
     6181 tgcggttatg ctggcgtcca ccgtaagttc ggattcagac ttaatcaaag gttttaacaa
     6241 ggcgctggca accccgcggc gggtgtattc agggtcaaca aataacatat cgatatgacg
     6301 ttcaatgcgg gaaataaaac caaccggttg tgcattaatg actgcaaccc gcacttgtga
     6361 tttcgcgagt ttctccttcc agcgagattc gtcaatctgc gcccaggcgg caatttgttg
     6421 tggtgaataa tgctgactgg cggtcatcat aaccgctcta atgaaaatag cgcatagttg
     6481 ctgaaaatcg ccaggctgat agtttcttat ttgtatgtta ttcataatcc cagcaccagt
     6541 tgtctttcca tttgagggtc aagcaacgtc acatgcagcc ccaccagtcg cacaccgcgc
     6601 ccgccgcggc gttcatccca ggttttacgc gcggtggcga ttagatcagc tttattcagc
     6661 cgcggccaga cgtgctcctg ggtggtttgc tgaaaatcgt cgaactttaa tttcaccccc
     6721 tggcgagcaa tcagtaaatc aggttttacc tttgccagac ggcgttcaag ttccggatac
     6781 agccgctcga taatcgcttc acattcagac cagtgatgaa tatcttccgc catcgtgcgt
     6841 tccacaccga cggattttcg caaccgttcg ctgttaacgt cgcgttcgtc aatcccctga
     6901 ctacgctccc acaaaatgcg gccaaatttg ccaaagcgtt taagcagaat caccagatca
     6961 cacttttgca catcaccgca ggtccgcagc cccatcgctt ccagttttgc cgccgagact
     7021 ttgccgacgc cggggatttt tgccaatggt aaggtttgca gaaatgccgg aacttctgcc
     7081 ggagtaatca caaactggcc gttgggttta ttcatgtcgg aggcgatttt ggcgagaaac
     7141 ttgaccggcg caacgcccgc agacgccgtc aggtgcagct cgttgaaaat cgtctggcgg
     7201 atttcctggg cgatgagggt cgcagaaccg tggcaatgga cgctatcggt gacatcgaga
     7261 taagcctcat ccagtgacaa cggttcaatg cgcgaggtgt agcgcgagaa gatttcacgg
     7321 atatgatttg aggcttcttt gtaggcgtca aagcgccccg gaagcaaggt gagatgtggg
     7381 cataatttga gcgccatccc agtaggcata gcgctacgta cgccaaattt acgcgcggga
     7441 taattggcgg tgctgatcac cccccgacgt tcgcggctgc cgccaatagc aatagggata
     7501 tcgcgcaggg cgggattgtc gcgcatctcc actgcggcga aaaagcagtc catatccaca
     7561 tgaatgattt tacgcattgc tcacctctca acactggtaa agtatacagt gatttcaggg
     7621 tttgagaaat gcgtaaagat tcagcattta gcgcattcgc tgcgaaagca cctgctgctt
     7681 atccagcttt tgcaccagcg gctgcaccac tttatcccca tgctgaccaa agtattgata
     7741 cagcgtatcg gacgcacttt tggtcagcac cataatctca atacgccgat tgcccgcgct
     7801 ttgcggattt ttggcatcca gcagcatctg gtccgccatt gcgcttacct gcatcacttt
     7861 atcttccggc attccggcct cttccagcac ccgacgagct gaaagcgcgc gatcgcccga
     7921 aaggttccag ttgttgtaga tattgttttt gtaggccatc gcatcggtat gcccggtaat
     7981 aataattttg ttatcgagcg agtcgaacac tggcgcgagc tccaccagca aagttttaaa
     8041 gaacggcata atctgcgcgc tgccgcattc gaacatattg cggttctggt catctttaat
     8101 cagcacgcgc aatccctggg gaacaatctc catctccaga ttcgcttcca tatgcgcatc
     8161 acgggcaatg gtgttgatac tggttgccag ctcgccaagc tcggtggcgg atttcttctt
     8221 cagcagcgct gttttctcgt tgacgtctcg cgcttttttc tccgtctctt ccggcaccgc
     8281 cacggtggca ggttttggcg gatgagacgg gctgatttta tttaacggcg acaatccgcc
     8341 gccactgaaa atcgactgcc cgtgcagcgc ggcgataatg ctttcacgct cggatttact
     8401 gacgctgttg acaatcaact ttatcgtcgt caccaaaggg gccgagcgta tttccgaggt
     8461 ttctgcccgc tttaccctgg acgcgatgcc cggcaaacag atggcgattg acgccgacct
     8521 taacgccggg ttgatcaacc aggcgcaggc gcaaacccgg cgtaaagatg tcgccagcga
     8581 ggccgatttc tacggcgcga tggacggggc gtcaaagttt gtgcgcgggg atgccatcgc
     8641 cgggatgatg attctggcaa tcaacctgat cggcggcgtc tgtatcggga tcttcaaata
     8701 caacctgagc gccgatgctg ccttccagca gtatgtgctg atgaccatcg gcgacgggct
     8761 ggtggcgcag atcccttccc tgctgctctc caccgcggcg gcgattattg tcacccgcat
     8821 cagcgacaac ggcgatatca cccatgacgt gcgccaccag ctgctggcaa gcccgtcggt
     8881 gctctacacc gctaccggga tcatgttcgt gctggcagtg gtgccgggga tgccgcacct
     8941 gccgtttttg ctgttcagcg ccctgcttgg ctttaccggc tggcggatga gcaaacgccc
     9001 gcaggcggcg gaggcggaag aaaaaagcct cgaaacgctg acccgcacca tcaccgaaac
     9061 cagcgagcaa caggtcagtt gggagacgat accgctgatc gagcctatca gcttaagcct
     9121 cggctacaag ctggtggcgc tggtggataa agcgcagggc aacccgctca cccagcggat
     9181 tcgcggcgtg cggcaggtga tatccgacgg taacggcgtg ctgctgccgg agatccgtat
     9241 tcgggaaaac ttccgcctca agcccagcca gtacgctatt ttcatcaacg gcattaaggc
     9301 tgatgaagcg gatattccgg cggataaact gatggcccta ccctccagcg aaacctacgg
     9361 cgaaattgac ggcgtgctgg ggaacgaccc ggcgtacggg atgccggtga cctggatcca
     9421 gcccgcgcag aaagcgaagg cgctgaatat ggggtatcag gtgatcgaca gcgccagcgt
     9481 gattgccacg catgtgaata agattgtgcg tagctatatt cctgatttat ttagctatga
     9541 cgatattacg cagttgcata accgtttgtc gtcgatggca ccgcgcctgg cggaagattt
     9601 aagcgcggcg ctgaattaca gccagttgct gaaagtgtac cgcgcgctgc tgaccgaagg
     9661 cgtttccctg cgcgatatcg tcaccattgc caccgtgctg gtcgccagta gcgcggtgac
     9721 caaagatcat attctgctgg cagctgatgt gcgcctggcg ctgcggcgaa gcattaccca
     9781 tccgttcgtt cgcaagcagg agctgacggt gtatacgctg aataatgagc tggaaaatct
     9841 gctgaccaac gtggtgaatc aggcgcaaca gggcgggaaa gtgatgctcg acagcgtgcc
     9901 ggtcgatccg aatatgctca accagttcca gagtacgatg ccgcaggtaa aagagcagat
     9961 gaaagcggcg gggaaagatc cggtgctgct ggtgccgcct cagctgcgcc ctttgctggc
    10021 gcgttatgca aggttgtttg cgccggggct gcatgtgctg tcgtataacg aagtgccgga
    10081 cgagctggag ttgaagataa tgggggcgtt gatgtaagta tccggcattg gtgcacaact
    10141 gccggatgcg gcgtgaacgc cttatccgtc ctactgttca ggcacgattc gtaggccgga
    10201 taagcgcaag cgcatccggc accagcgcac aaccgccgga tgcggcgtaa gcgccttatc
    10261 cggcctaccg atccagcaca ggtttgtagg catgataaga cgcgtcagcg tcgcatcagg
    10321 catctgcgca taaccgccgg atgcggcgta aacgccttat ccggcctacc gatccggcac
    10381 aggtttgtag gcatgataag acgcgccagc gtcgcatcag gcatctgcgc ataattataa
    10441 aatgatacgc tcgccagcat ttatatccgt tacgtccccc gcccaatcga tgggatataa
    10501 ccctcgcgcg acatcgcgat ggaacgttga gaatggccaa tcactcactt gctttaccca
    10561 accatgcttt actggattta tataaatata atcaacatga tgcctataat ctttggtatt
    10621 gcggatggcg tgctcccaaa aacgtggttg ccagatattt ttcaatccac aagcatgggt
    10681 aaattgcttt ttaatttccc gccagcgcga ggaaaaatca tcatcgcctt caggtaatgt
    10741 ccagatacag tgcatatgct ctggcaaaac gacccaggcg ttgatttcaa aaggcctgtc
    10801 tcgcttaact ttaataatgg cgtgacggag catctggtac tgggtggtca agagttggct
    10861 tcgacgattg cgtaaattca ccgtgaaaaa ccaggttccc cccttgatgt aataacgacg
    10921 atattcagac atggcgactc ctttttctca acggtaagaa gaaaaggaaa ggtcggtaat
    10981 gaggtgatgg aataacagga aagcggctcg caaaaacgca cagatttgta ggcatgataa
    11041 gacgcgtcag cgtcgcatca ggcatccgtg cacaaccgcc ggatgcggcg taaacgcctt
    11101 atccggccta caaaatcgtc tcttccgtca agatccgccg cgcgcccaaa aaatgatcct
    11161 gccagaaagg ttcggctaac cggcttaccc gaatgttttc cccggtgcgc ggcgactcga
    11221 taaactgccc atcgcccaga tacaccccca tatgatcggc aatttcacgg ctgtggatat
    11281 ggaaaaacag caaatctccc cggcgcaggt cgttattcgc cacaatcgtt gcccgatgat
    11341 agtgatacat ctcattggcc gtgcgcggga gcttcgcctc aaggatcttg ttgtaggcat
    11401 aaaaaaccaa cccgctacag tcgaagcctt gatcgggccg cgtgccgccc cagacgtaag
    11461 gcttgccaag ctgttgctcc agacggtgaa tcgccacttc ggtgatgtta tgcagatggt
    11521 cgctgcttaa aaagtgattg ttctccgcca gcgcctgcca gcgtcggtcg ctcgccttga
    11581 gcggcgcagg aaaccattct ggatgctgtt ttatctgctc ccggttgtgc tggcgtagcg
    11641 cccttctgct ttcggcattg ccttccacaa tatagctggc ctgttttttc agatgggttt
    11701 ggtactgttt taataaacgc gcacggtttt gcatccgctg gcgggcggcg tagctgaaag
    11761 agatatgtga tgtggtggcg ctggcgaata aagggagcaa aacgcataac gaaaacacgc
    11821 ctggatgcag aaatctgccg agcaagaacg aagacatgaa agacataaac cggctgaatg
    11881 aaggtgagaa ttttaaaaga ccggaggagt aaacgggatt attaattgct tatttgcata
    11941 ttactattga ggtttcataa tcttaggaaa taaatgatac atgcgctttt cggactattg
    12001 aaaactgccc ctgtgctaaa tttgtaaacc ctgcgccgac aggcttgcgt aaaactcttt
    12061 tggtgataat gtgagccttc caaatccaga ctattccgat ttgggatcca ttttgccgtc
    12121 ctggcgtgtg taaccgtttt atcaaggaat aagcagtatg cgtaaaatcg cattaattct
    12181 tgcgatgttg ttgatcccgt gcgtttcgtt tgccggtttg ctgggcagca gtagctcgac
    12241 cacgccagtg agcaaagagt ataagcagca gttgatggga tcccctgtct acatccagat
    12301 cttcaaggaa gaacgtacgc tcgatctcta cgtcaaaatg ggcgagcaat atcagctgct
    12361 cgacagttat aaaatctgta aatattccgg cggcttaggg ccaaaacagc gtcagggcga
    12421 tttcaaaagc ccggaagggt tttatagcgt ccagcgtaat cagttaaaac cagacagccg
    12481 ttactacaaa gcgattaata ttggtttccc caatgcctat gaccgtgcgc atggttatga
    12541 agggaaatac ctgatgatcc acggcgattg tgtttccatc ggctgctacg cgatgaccaa
    12601 tcagggcatt gacgagattt tccagttcgt tactggcgca ctggtatttg gtcagccgag
    12661 cgtgcaagtg agcatttacc cgttccgcat gaccgacgct aatatgaagc gccacaaata
    12721 ttccaacttt aaggacttct gggagcaact gaagccgggt tacgactact ttgagcaaac
    12781 ccgtaagcca ccgaccgttt ctgtggtcaa tggtcgttac gtggtcagca agccgttaag
    12841 ccacgaagtt gtgcaaccac agctggcatc aaactacacg ctccccgagg caaaataagc
    12901 gccattcgcc tggcataatt ttttgccagg tttcattgcc cgtcagcggc tgtgtcgcaa
    12961 tcaccgtgac cacatcattc ggtgtggtct gcgagctgaa gtcgatttcc acatcctgat
    13021 ccagcaacgt tgccacgcca aacggcgcgc ggcgggtgat ccagtgtaaa ttagtcgagc
    13081 aatacgccat tacatagcgc ccgtcagaaa gcagcatgtt gaaaacgccc ttctgccgca
    13141 gttcatccgc cagtgaggcg atatatttaa ataccgccgc catgttgccc ggtgtgcgcg
    13201 ggtaacgctg cgttaattta tgcaggagcc agcaaaaggc tttttcgctg tcggtttcgc
    13261 ctaccgggcg gaagttgccg gtttccagtg atttgtagcc cgtcagttgt ccgttatggg
    13321 cataagtcca gttacgcccc cataactcgc gggtaaatgg gtgagtattt tccagcgcca
    13381 cctcgccccg attagcctgg cgaatatgag ccaccaccga acaggatttt atcgggtagt
    13441 cctggacaag tttggcgatg ggggaattaa agctgggttg tggatcttta aatgtgcgac
    13501 agcctttacc ttcgtaaaag gtaatgcccc agccatcttt atgtggcccg gttccaccac
    13561 cacgctgtac aagcccggtg aaactaaagc agatatcggt agggacgttg gcgctcatcc
    13621 cgagcaattc gcacatgggt aacctccaca acagcgggag cgcatggccc ccggcgaaga
    13681 ttgatttaag aattttcagg tcggatgcgg cgtgaacgca ttattcggcc tacggttcgc
    13741 gtacagattt gtaggcatga taagacgcgt cagcgtcgca tcaggcatcg gtgcacaatt
    13801 gccggatgcg gcgtaaacgt cttatccggc ctacgccaga cttacttaac catctctttt
    13861 tcaatcaact ggatcaggat atggatcact ttaatgtgaa tctcctgaat gcggtcggca
    13921 taaccaaagt gcggtacgcg aatttcgata tccgccgtgc cagccatttt gccgccgtct
    13981 ttaccggtca gggtgatcac tttcattccc ttctcacgcg ccgctgcgat cgctttgatc
    14041 acgtttgcag agttaccgga ggtggagatc cccagcagta catcgccttc gcgacctacc
    14101 gcttcaacgt aacgggagaa aatatcattg aaaccgaaat cgttaccgac gcatgaaata
    14161 tgactaacgt cagaaatagc aatcgccggg tagcccggac ggttttcacg gtagcgaccg
    14221 gtcaactctt cggcaaagtg catagcgtcg caatgggaac cgccgttgcc gcaggaaagc
    14281 actttgccac cggctttaaa gctgtctgct aacaggaccg ccgcgcgctg aatggcgtga
    14341 atattggcgt catcttttaa aaagttagcc agcgtttccg ccgcttcgtt cagttcgtta
    14401 cgaataagat cctggtacat gaggatatcc ttcagcataa atgtaataga caaaatgcag
    14461 tgtaccggat accgccaaaa gcgagaagta cgggcaggtg ctatgaccag gactttttga
    14521 cctgaagtgc ggataaaaac agcaacaatg tgagctttgt tgtaattata ttgtaaacac
    14581 attgctaaat gtttttacat ccactacaac catatcatca caagtggtca gacctcctac
    14641 aagtaagggg cttttcgtta tgatgatttt gagtattctc gctacggttg tcctgctcgg
    14701 cgcgttgttc tatcaccgcg tgagcttatt tatcagcagt ctgattttgc tcgcctggac
    14761 agccgccctc ggcgttgctg gtctgtggtc ggcgtgggta ctggtgcctc tggccattat
    14821 cctcgtgcca tttaactttg cgcctatgcg taagtcgatg atttccgcgc cggtatttcg
    14881 cggtttccgt aaggtgatgc cgccgatgtc gcgcactgag aaagaagcga ttgatgcggg
    14941 caccacctgg tgggagggcg acttgttcca gggcaagccg gactggaaaa agctgcataa
    15001 ctatccgcag ccgcgcctga ccgcagaaga gcaagcgttt ctcgacggtc cggtagaaga
    15061 agcctgccgg atggcgaatg atttccagat cacccatgag ctggctgatt tgccgccgga
    15121 gttgtgggcg taccttaaag agcatcgttt cttcgcgatg atcatcaaaa aagagtacgg
    15181 cgggctggag ttctcggctt atgcccagtc tcgcgtgctg caaaaactct ccggcgtgag
    15241 cgggatcctg gcgattaccg tcggcgtgcc gaactcatta ggcccgggcg agctgctgca
    15301 acattacggc actgacgagc agaaaaatca ctatctgccg cgtctggcgc gtggtcagga
    15361 gatcccttgc tttgcactga ccagcccgga agcgggttcc gatgcaggcg ctatcccgga
    15421 taccgggatt gtctgcatgg gcgaatggca gggccagcag gtgctgggga tgcgtctgac
    15481 ctggaacaaa cgctacatta cgctggcacc gattgcgacc gtgcttgggc tggcgtttaa
    15541 actctccgac ccggagaaat tgctgggtgg cgcagaagat ttaggtatta cctgtgcgct
    15601 gatcccaacc accacgccgg gcgtggaaat tggtcgtcgc cacttcccgc tgaacgtgcc
    15661 gttccagaat ggaccgacgc gcggtaaaga tgtcttcgta ccgatcgatt acattatcgg
    15721 cgggccgaaa atggccgggc aaggctggcg gatgctggtg gagtgcctct cggtaggccg
    15781 cggcatcacc ctgccttcca actcaaccgg cggcgtgaaa tcagtagcgc tggcaacggg
    15841 tgcgtatgcg cacattcgtc gtcagttcaa aatctctatt ggtaagatgg aagggattga
    15901 agagccgctg gcgcgtattg ccgggaacgc ttatgtaatg gatgctgcgg catcgctgat
    15961 tacctacggc attatgctcg gcgaaaaacc tgccgtgctg tcggctatcg ttaagtatca
    16021 ctgtactcac cgcgggcagc agtcgattat tgatgcgatg gatatcactg gtggcaaagg
    16081 cattatgctc gggcaaagca acttcctggc gcgtgcttac cagggcgcac cgattgccat
    16141 caccgttgaa ggggctaaca ttctgacccg cagcatgatg atcttcggac aaggagcgat
    16201 tcgttgccat ccgtacgtgc tggaagagat ggaagcggcg aagaacaatg acgtcaacgc
    16261 gttcgataaa ctgttgttca aacatatcgg tcacgtcggt agcaacaaag ttcgcagctt
    16321 ctggctgggc ctgacgcgcg gtttaaccag cagcacgcca accggcgatg ccactaaacg
    16381 ctactatcag cacctgaacc gcctgagcgc caacctcgcc ctgctttctg atgtctcgat
    16441 ggcagtgctg ggcggcagcc tgaaacgtcg tgagcgtatc tcggcacgtc tgggtgatat
    16501 tttaagccag ctttacctcg cctctgccgt tctgaagcgt tatgacgacg aaggccgtaa
    16561 tgaagccgac ctgccgctgg tgcactgggg cgtacaggat gcgctgtatc aggcagaaca
    16621 ggcgatggac gatttactgc aaaacttccc gaaccgcgtg gttgccgggc tgctgaatgt
    16681 ggtgatcttc ccgaccggac gtcattatct ggcaccgtct gacaagctgg atcataaggt
    16741 ggcgaagatt ttacaagtgc cgaacgccac tcgctcacgt attggtcggg gtcagtacct
    16801 gacgccgagc gagcataatc cggttggttt actggaagag gcgttggtgg atgtgattgc
    16861 cgccgacccg attcatcagc ggatctgtaa agagctgggt aaaaatctgc catttacccg
    16921 tctggatgaa ctggcgcaca atgcgctggc gaaggggctg attgataaag atgaagccgc
    16981 tattctggtg aaagcggaag aaagccgtct gtgcagtatt aacgttgatg actttgatcc
    17041 ggaagagctg gcgacgaagc cggtaaagtt gccggagaaa gtgcggaaag ttgaagccgc
    17101 gtaattgaat aacggagccg aaaggctccg tttctttatc cgctaattat ttaaaattaa
    17161 agccatccgg atggttttcc aggctgccgg tcaacgccgc gaacagcacc gttttaccat
    17221 caatcgaaag cgcatcgttc acattcagcc aggtgagttt ctcttgcgac gttttctcat
    17281 caatagtcga gaacagcccc gtcatctgat tagatttctc ggaccacatc acagcgatac
    17341 gttgcgagcc acagtcatgc ggtttgcacg cgctcatcac ctgatacgtc tcatctccca
    17401 acgttacggt ttgtgcggga gtataagtac cgcctttcat cacccatgca ggtagcttat
    17461 gcccttgcac catctgatta aacgcagctt tggtggtttc gccctttgcc aggctgctaa
    17521 tggttaaatc atcctgcgcc attgcactgg tggcgatgac cagagcggcg actgtcgtta
    17581 ttgccttaaa catcattcct cccgagctta tcctgcccat gttattaacc tcctaaagcc
    17641 tggcgataac acgataattt gcaaatcaga tatttccaat atcagcattt gttatcaatt
    17701 tattctgcgc taaggcaact gcctgataag gtgttaaagt tataaaaccc ttcgcataaa
    17761 ggagttgtac accgtgcctg gtttgaagat tacgcttttg cagcaaccac tggtgtggat
    17821 ggatggccct gccaacctgc gtcattttga tcgtcaactg gaaggtatta ccgggcgcga
    17881 tgtgatcgtt ctaccggaga tgtttaccag cggctttgcc atggaagcgg cagcttcgtc
    17941 gctagcacaa aatgacgtag tgaactggat gacagctaaa gcgcagcagt gcaatgcgct
    18001 gattgcaggc agtgttgcat tacaaacgga gtctggttcg gttaaccgct ttttgctggt
    18061 tgagccgggc ggcacggtac atttttatga taagcgtcat ctgttccgca tggcagatga
    18121 gcatctgcat tataaagcgg gcaatgcgcg agtgattgtg gaatggcgcg gctggcgtat
    18181 tttgccgctg gtgtgctacg acttacgttt tcctgtgtgg tcgcgcaatc tcaacgatta
    18241 tgacctcgcc atctacgttg ccaactggcc tgctccgcgc tctctgcact ggcaggcatt
    18301 gctgacggcc cgcgcgattg agaatcaggc gtatgtggcg ggatgcaatc gcgtcggcag
    18361 cgatggcaac ggctgccatt atcgcggtga cagccgggtg attaacccgc agggcgagat
    18421 tatcgctact gccgacgcgc atcaggcaac gcgcattgat gcggagctgt cgatggtggc
    18481 attgcgggaa tatagagaaa agtttccggc atggcaggat gcggatgagt ttaggttgcg
    18541 gtgagtgggg gttgtgttta aggctggggg agagtcgggg taagactacg aaagcccgct
    18601 ctccgcaagg actgacgcca gatagtttct gtccatagct gcttttcgca tcttacgtct
    18661 taaccctgcc ttgaatacct tatcattcgt caaaatatta atagcgatgt gccgtatccc
    18721 tgaaaataat tctgcggcgt ttcctcttct tattttgcag tcgtcttcat tcattaccac
    18781 gtccagacgc cagtgcagct tattctccac gtgccagtgg tttcggattg ctgtggcgaa
    18841 cttttctgcg gttaaatcag cagaactgat ataatatctg accgtcattt ctggctcttt
    18901 cttttgttct gctattattg accgaaagga gactgccacg cataatttct tcagtccttt
    18961 ccattcaaac gtgaaatcaa taagttcatc agggacatcg caaacaatat gaagacggat
    19021 ttcttctctg ccgtgactct tttcact
//
LOCUS       DAFNBJ010000092        18990 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000092 DAFNBJ010000000
VERSION     DAFNBJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.092"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..97)
                     /locus_tag="L2X49_003062"
     CDS             complement(<1..97)
                     /locus_tag="L2X49_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBN4798108.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKG"
     gene            complement(99..458)
                     /locus_tag="L2X49_003063"
     CDS             complement(99..458)
                     /locus_tag="L2X49_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBN4798109.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            679..754
                     /locus_tag="L2X49_003064"
     tRNA            679..754
                     /locus_tag="L2X49_003064"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:712..714,aa:Ala,seq:ggc)
     gene            794..869
                     /locus_tag="L2X49_003065"
     tRNA            794..869
                     /locus_tag="L2X49_003065"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:827..829,aa:Ala,seq:ggc)
     gene            1077..3266
                     /locus_tag="L2X49_003066"
     CDS             1077..3266
                     /locus_tag="L2X49_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HBN4798110.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(3316..4518)
                     /gene="nupC"
                     /locus_tag="L2X49_003067"
     CDS             complement(3316..4518)
                     /gene="nupC"
                     /locus_tag="L2X49_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBN4798111.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            4854..6092
                     /locus_tag="L2X49_003068"
     CDS             4854..6092
                     /locus_tag="L2X49_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBN4798112.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(6233..6559)
                     /locus_tag="L2X49_003069"
     CDS             complement(6233..6559)
                     /locus_tag="L2X49_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBN4798113.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(6674..7930)
                     /locus_tag="L2X49_003070"
     CDS             complement(6674..7930)
                     /locus_tag="L2X49_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBN4798114.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLLEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            8134..9099
                     /gene="glk"
                     /locus_tag="L2X49_003071"
     CDS             8134..9099
                     /gene="glk"
                     /locus_tag="L2X49_003071"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBN4798115.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHNNPGLLGSGAHLRQTLGHIL"
     gene            9318..9644
                     /locus_tag="L2X49_003072"
     CDS             9318..9644
                     /locus_tag="L2X49_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBN4798116.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            9666..10913
                     /locus_tag="L2X49_003073"
     CDS             9666..10913
                     /locus_tag="L2X49_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBN4798117.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESRITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            10928..12012
                     /gene="ypdF"
                     /locus_tag="L2X49_003074"
                     /pseudo
     CDS             10928..12012
                     /gene="ypdF"
                     /locus_tag="L2X49_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            12012..13049
                     /gene="ypdE"
                     /locus_tag="L2X49_003075"
     CDS             12012..13049
                     /gene="ypdE"
                     /locus_tag="L2X49_003075"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBN4798118.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            13074..15569
                     /gene="ptsP"
                     /locus_tag="L2X49_003076"
     CDS             13074..15569
                     /gene="ptsP"
                     /locus_tag="L2X49_003076"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBN4798119.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(15572..16429)
                     /locus_tag="L2X49_003077"
     CDS             complement(15572..16429)
                     /locus_tag="L2X49_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4798120.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(16442..17176)
                     /gene="ypdB"
                     /locus_tag="L2X49_003078"
     CDS             complement(16442..17176)
                     /gene="ypdB"
                     /locus_tag="L2X49_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBN4798121.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(17191..18888)
                     /gene="ypdA"
                     /locus_tag="L2X49_003079"
     CDS             complement(17191..18888)
                     /gene="ypdA"
                     /locus_tag="L2X49_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBN4798122.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         4482 a         4739 c         5076 g         4693 t
ORIGIN      
        1 agcctttttc tctgatccaa cggttaacag tttgtttagc cacaccgagg cattcagcca
       61 gttcttcggt ggtcattttg ttgcgtaatc ttttcatatt atttgctatc gcgaatccct
      121 aagcgttgta acaatccgat aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt
      181 tcaactggcg tcatctcttt cgccagtgtc accagcaata cttcaagcgg cgcatcacca
      241 gaaagggcag gcgcttctcc ctgaccttct ggacgttcag cattgcgaat atattcacga
      301 acctgttcat tgacgtgaac cagtcgggct ttgccaccct ggacgccagg ttttggtgac
      361 gttgtccagc cttccttgcg tacccattta ttaatggtct ggcggctata gccggtcagt
      421 ctggcaagtt catctggtgt catcctctcc ttgaacataa caattccctg aatgtatgtg
      481 ggtctattag tagttcattt tataacacca ctttacagga aaccgtgaca tgaataacta
      541 ctgaatgcga gcggttttcc aaaattgttc atttgcctga tttttcagcg attgaaacta
      601 cacgttgcaa tttgccgttg acacattcgg gcggaattca tatgatgccg cccgtcaact
      661 cgacaagctt tacgtgacgg ggctatagct cagctgggag agcgcttgca tggcatgcaa
      721 gaggccagcg gttcgatccc gcttagctcc accaaatttt gcacccagca aatttggtac
      781 gtaaacgcat cgtggggcta tagctcagct gggagagcgc ttgcatggca tgcaagaggt
      841 cagcggttcg atcccgctta gctccaccaa atttccaacc ctcgctgcaa agcgggggtt
      901 ttttgtctct gctttttgcc gcttttgtaa tacagtctac gtccgggtta gcgccgcctg
      961 gtgaaagcat cattggatga aaaatcggca acaggctggc ccctgtttgc ttcgcgatgc
     1021 gaataaactt attatttgtg tgcctgaaaa ccccgatcag tgagagtagt gtactcatgt
     1081 ttgtggagca taacctgata aaaaatatca agatattcac actagcgttt acgctcaccg
     1141 tggtacttat tcagctatcc cgttttattt cgccacttgc cattatccat tccagttata
     1201 tctttctggc gtggatgcca ctgtgcgtaa tgctgtcaat cttgtttatc tttggctggc
     1261 gcggtgtcgt tcccgtttta tgcgggatgt tttgcaccaa tctgtggaac tttcatctct
     1321 cttttttaca gactgcggtc atgctcggta gccagacgtt tgtcgtgttg tgtgcctgcg
     1381 caatattacg ctggcagctg gggacgcgtt ggcgttatgg attgaccagc cgatatgtct
     1441 ggcaacgtct gttctggctt ggtttggtga cgccgatcgg catcaaatgc agcatgtatc
     1501 ttgtggggag cttctttgat ttcccgctaa agatatccac ctttttcggc gatgcggatg
     1561 ccattttcac ggtcgttgat ttgctaagcc ttttcaccgc tgtgctgatt tacaacatgc
     1621 ttttctacta tctcactcgc atgattgtaa gtccccactt tgcgcagata ttgtggcgca
     1681 gggatatcgc tccgtcgttg ggcaaagaga aacgcgcatt taccttaagc tggctggcag
     1741 cgttaagcgt gctgctactt ctgttgtgca caccttatga aaacgacttt attgccggtt
     1801 acttggtacc cgttttcttc atcatcttta ccctcggggt cggtaagctt cgctatccgt
     1861 ttttaaatct cacctgggct gtttcaacgc tttgcctgct gaattacaac cagaactttt
     1921 tgcaaggggt ggaaaccgaa tattcgctgg catttattct tgcggtgctg atttccttta
     1981 gcgtttgcct gctctatatg gtgcgcattt atcatcgcag tgaatggctt aatcgccgct
     2041 ggcatttgca ggcgctgaca gatccgttaa cgctcctacc caactttcgt gcgttggaac
     2101 aagcgccgga gcaagaggcg ggcaagagtt tttgctgcct gcgcattgat aatcttgagt
     2161 ttatgagtcg tcattacggc ttaatgatgc gcgttcactg tatccgctca atttgccgta
     2221 cgctgctgcc gttgatgcag gaaaacgaaa agttgtatca attgccgggt agtgaactgc
     2281 tgttagtgct gagcgggccg gaaacggaag ggcgactcca gcatatgatt aacatcctga
     2341 atagtcggca aattcactgg aacaataccg ggctggatat gggctatggt gctgcctggg
     2401 ggcgttttga tggaaatcag gaaaccctgc aacccttgtt ggggcagtta agctggctgg
     2461 cggagcaatc ctgcgcacat catcatgtgc tggcgctgga tagcagagag gagatggttt
     2521 ccgggcagac cactaaacag gtgctattgc tgaataccat tcgcacggcg ttagatcagg
     2581 gtgatttgct gctctacgcc cagccaattc gcaacaaaga gggtgaaggt tatgatgaga
     2641 tcctcgcgcg actgaaatat gacggcggca ttatgacccc ggataagttt ctgcccctta
     2701 ttgctcagtt taaccttagc gcgcgttttg atttgcaagt gctggaatcc ttgttgaagt
     2761 ggctggcaac acacccttgc gacaaaaaag gaccgcgctt ttcagtcaat ttaatgccgc
     2821 tcacgctgct gcaaaagaat attgccgggc ggattattcg tctgtttaag cgttatcaca
     2881 tctccccgca ggcggtcatt cttgagatca ccgaggagca ggcgttttct aacgcagaaa
     2941 gcagcatgta caacatcgag cagctgcata agtttggttt ccggattgcg attgatgact
     3001 ttggcaccgg atatgccaac tacgaacggt taaagcgttt gcaggctgat atcatcaaaa
     3061 ttgatggcgt ctttgtgaaa gatattgtca cgaacacgct ggatgcgatg attgtgagat
     3121 caattaccga tctggcgaaa gcgaagtcat tgagtgtggt cgcggagttt gtcgagacgc
     3181 aacagcagca ggcgctattg cataagctcg gggtgcaata tctgcaaggg tatttgattg
     3241 gtcgcccgca gccattagct gattaacagg cgtaaaaaaa ccggggaatt atccccggct
     3301 tttttatgta tggttttaca gcaccagtgc tgcgattgac gcagacagca cactcaccag
     3361 ggtagagccg taaaccagtt tcagaccgaa gcgagaaacc acgttacctt gctcttcatt
     3421 caggccttta actgcacctg cgataatccc gattgaagag aagttagcga aggaaaccag
     3481 gaacacagag attatgcctt cagcacgcgg agagagcgtg gaagcaattt tctgcagatc
     3541 catcatcgca acgaactcgt tggaaaccag tttggtcgcc atgatactgc ccacttgcag
     3601 tgcttcactg gaaggaacac ccatcaccca tgcaatcgga tagaagatgt agcccaggat
     3661 gccctggaag gagatgctgt agccaaacca gccagtaacg gtggcaaaca gtgcgttcag
     3721 cgcggcgatc agggcgataa agccgatcag catcgcggca acgataatgg caactttgaa
     3781 acctgccaga atgtattcac ccagcatttc gaagaagctc tgaccttcgt gcaggttgga
     3841 catctggatg ttttcttcac tggcatcaac acggtaagga ttgatcagcg acagcacgat
     3901 aaaggtgctg aacatgttca gtaccagcgc agcaacgacg tatttcggtt ccagcatggt
     3961 catgtatgca ccaacgatgg acatcgacac ggtggacatt gccgtggcag ccatggtgta
     4021 catacgatta cgggagattt tgccgaggat atctttatag gcaataaagt tttcagactg
     4081 acccagaatc agggagctga cggcgttaaa ggattccagt ttgcccatac cgttgacttt
     4141 ggagagcagg aaaccaattg cgcggataat aaccggcaac acgcgaatgt gctggagaat
     4201 accgatcagt gcagagataa agacgattgg gcacagcact ttcaggaaga agaatgccag
     4261 gccttgatca ttcatgctac caaagacgaa gttagtccct tcgttggcaa atccgagcag
     4321 tttttcgaac atttcggaga agcctttcac gaagcctaaa ccaacgtcgg agttcaggaa
     4381 gaaccacgcc agtaacactt cgataacaag cagttgaata acataacgga tacgaatttt
     4441 tttgcggtcg ctgcttacca gcagtgcgag aatcgcaaca acggcaagtg ccagtacaaa
     4501 atgaaggacg cggtccatat ttgctccaaa tatgaggcag gttaaatttc cgtgcacatt
     4561 ctatgtaaca tggttaagga aaacgagatc tgacacacac tataatgacg tcctgtgaca
     4621 agcttcaaaa atagtgatct gtcatacatt tctgctatgt tgtgtatgga agctgaaagt
     4681 tatgtaaatg tgctaacatt aaacataacc ataacattca agtcatcaat cttttcgtag
     4741 tcataatcct ggtctatcaa agaaatcacc acaatccatt caaatgaatt tgataatcat
     4801 tctcgtttgg catagcatga aacatagcaa aggctatgtt ttagaggcac aagatgacga
     4861 actatcgcgt tgagagtagc agcggacggg cggcgcgcaa gatgaggctc gcattaatgg
     4921 gacctgcgtt cattgcggcg attggttata tcgatcccgg taactttgcg accaatattc
     4981 aggcgggtgc tagcttcggc tatcagctac tgtgggttgt cgtttgggcc aacctgatgg
     5041 cgatgctgat tcagatcctc tctgccaaac tagggattgc caccggtaaa aatctggcgg
     5101 agcagattcg tgatcactat ccgcgtcccg tagtgtggtt ctattgggtt caggcagaaa
     5161 ttattgcgat ggcaaccgac ctggcggaat ttattggtgc ggcgatcggt tttaaactca
     5221 ttcttggtgt ttcgttgttg cagggcgcgg tgctgacggg gatcgcgact ttcctgattt
     5281 taatgctgca acgtcgcggg caaaaaccgc tggagaaagt gattggcggg ttactgttgt
     5341 ttgttgccgc ggcttacatt gtcgagttga ttttctccca gcctaacctg gcgcagttgg
     5401 gtaaaggaat ggtgatcccg agtttaccta cttcggaggc ggtcttcctg gcagcaggcg
     5461 tgttaggggc gacgattatg ccgcatgtga tttatttgca ctcttcgctc actcagcatt
     5521 tacatggcgg ttcgcgtcaa caacgttatt ccgccaccaa atgggatgtg gctatcgcca
     5581 tgacgattgc cggttttgtc aatctggcga tgatggctac agctgcggcg gcgttccact
     5641 tttctggtca tactggtgtt gccgatcttg atgaggctta tctgacgctg caaccgctgt
     5701 taagccatgc tgcggcaacg gtctttggat taagtctggt tgctgccgga ctgtcctcaa
     5761 cggtggtggg gacactggcg gggcaggtgg tgatgcaggg attcattcgc ttccatatcc
     5821 cgctgtgggt gcgtcgtaca gtcaccatgt tgccgtcatt tattgtcatt ctgatgggat
     5881 tagatccgac acggattctg gttatgagtc aggtgctgtt aagttttggt atcgccctgg
     5941 cgctggttcc actgctgatt ttcaccagtg acagcaagtt gatgggcgat ctggtgaaca
     6001 gcaaacgcgt aaaacagaca ggctgggtga ttgtagtgct ggtcgtggcg ctgaatatct
     6061 ggttgttggt ggggacggcg ctgggattgt agttgaatga gcgtcgcatc tggcactatt
     6121 ggcggatgcg gcgtaaacgc cttatccgcc ctacgcgtta aagagccggt ttgtaggcct
     6181 gataagacgc gctagcgtcg catcaggcat tgtgttccat tggcgaatgt gtttaatggc
     6241 ggtgtccatg tccgcggcct tttccgcgat gatcgtcacg atcgcgccag ccttcacgat
     6301 agccacgctc atacgcttta cgcttatccc agccacgatg atagccatta tcatgacgcc
     6361 accagcggtt tttacgccac tcataattgc gatgccagta gtcacggtcg cgccagtgac
     6421 caccgtccca gtaattaccg taatgatcgc gatcgccaat ttgtaatttg attgatggca
     6481 gtagggtgat ttcacctgcg tttgctgcaa gcggggtaaa tgccatcagg gcggccgcca
     6541 gaaacagtga cctgaacatt gttattctcc ttcacgatcg aagccgtcag cggcctgtta
     6601 acgcaatatt acggggaggt aaagccccgt atcatcgcca taactcttaa atcataaagg
     6661 agagcatttt ttgctaaacg ttatcgtggg gtggctgttg cttcggacga ttgaccatca
     6721 tcatcggctt acctgctaat aacagccatg ccgggagcat gacgatacag agcagcggta
     6781 gcaatgtggt attgggtaca acgaccgccg ccataaaaag acttaaccag ccatcgcgtg
     6841 ttaccaccag cacgatgccg agaatagcgc aagaaacggt tattgccgct ggtacggcgg
     6901 gtacgtgctc aagcagcatc aaccctaatg ccacgccgac aaacactgcc gggaagattc
     6961 gcccaccgcg aaagccactg gcggcagcaa cgaccagggc ggcaagttta attaccgcca
     7021 gcaaaaagta atcgctggtg ctgaaagcct gatttgccac catctgctgc atctcatcca
     7081 gccctttaaa cagcgaaact ggtccaccaa taacccccag aataccgaga ataaatccgc
     7141 caatacccag cacgagcacc ggatttttca tttgattcat catcgcgtgc aaccgtggta
     7201 agcaccatac ggcgaccatt cctgctgcga tggcaatcgc cgcgacaatt gcaccgctga
     7261 gaatatcggt catttccatc tgtccgtaat gcgcaatggg cagtgaaaaa tggggatgga
     7321 aaaataatcc ggtagtaagt gcaccagctg ctgccgccat taatggcgca aaaagacgat
     7381 cccatagcgg aacttcacta ctgccattta aggtttgcga aaatatcaac gccgccgcaa
     7441 caggtgtacc aaacagcgcg ccgatggttc cggcagaggc taaaatagtc cactccattc
     7501 ggttgacgcg cggtaacaga cgagcgccaa tggcaaccgc aagggcgata ttgacggtca
     7561 tgatcggatg ttccggcccc aggctgacgc cgccagcaag accgagaatt aatgctacga
     7621 taagtcctgg tagcgcagag ggcggaactg gtgcgccgat cagcggttca caggcggggt
     7681 ctggtccggc atgaccctgg ctgaaacgga taaccaaccc caccgcaata cccgttagcg
     7741 ttaatacacc gatgatccag agaggtgaat cctgggctat ccccagagtt cccggcagtc
     7801 gttgccagag caaattctgt aataccgagg cgattttcat caccacaatc agaataagac
     7861 tggacgcaat cccaattgcc acggcgggga gcgataataa caacatggtt ctggctcgcg
     7921 gatggagcat gttctattcc ttatgcgggg tcagatactt agtttgccca gcttgcaaaa
     7981 aggcatcgct gcaattggtg ctgaaacgat aaagtaaatg tgtgacccag atcgatattt
     8041 acagggagcc tgcctttccg gcgttgttgt tatgccccca ggtatttaca gtgtgagaaa
     8101 gaattatttt gactttagcg gagcagttga agaatgacaa agtatgcatt agtcggtgat
     8161 gtgggcggca ccaacgcacg tcttgctctg tgtgatattg ccagtggtga aatctcgcag
     8221 gctaagacct attcagggct tgattacccc agcctcgaag cggtcattcg cgtttatctt
     8281 gaagaacata aggtcgaggt gaaagacggc tgtattgcca tcgcttgccc aattaccggt
     8341 gactgggtgg caatgaccaa ccatacctgg gcgttctcaa ttgccgaaat gaaaaagaat
     8401 ctcggtttta gccatctgga aattattaac gattttaccg ctgtatcgat ggcgatcccg
     8461 atgctgaaaa aagagcatct gattcagttt ggtggcgcag aaccggtcga aggtaaacct
     8521 attgcggttt acggtgccgg aacagggctt ggggttgcgc atctggtcca tgtcgataag
     8581 cgttgggtaa gcttgccggg cgaaggcggt cacgttgatt ttgcgccgaa tagtgaagaa
     8641 gaggccatta tcctcgaaat attgcgtgcg gaaattggtc atgtttcggc ggaacgcgtg
     8701 ctttctggtc ctgggctggt gaatttgtat cgcgcaattg ttaaagccga caaccgcctg
     8761 ccagaaaatc tcaagccaaa agatattacc gaacgcgcgc tggctgacag ctgcaccgat
     8821 tgccgccgtg cgttgtcgct gttttgcgtc attatgggcc gttttggcgg caatctggca
     8881 ttaacgctgg gaacctttgg tggggtttat attgctggcg gtatcgtgcc gcgcttcctt
     8941 gagttcttca aagcctctgg tttccgtgcc gcatttgaag ataaaggacg ctttaaagaa
     9001 tatgtccatg atattccggt gtatctcatc gtccataaca atccgggtct tctcggttcc
     9061 ggtgcacatt tacgccagac cttaggtcac attctgtaaa tccttcctgt tatatcggga
     9121 ggtaactctc ccgataatct tttaaatcat acagttaatt caatttttct ttgtgtcccc
     9181 tcacaaggcc gacctgcgtc acacttccgt acagcgggat taattctcca gtaaatgcat
     9241 tatttgtctg gtaacggcga tttgttttgc acgttcataa tttcactcgt caactatcac
     9301 tgaatgagga attaacgatg agtaagaaac tgattgcctt atgtgcctgc ccaatgggcc
     9361 tggctcacac ctttatggcc gctcaggcgt tggaagaagc ggcggtagaa gccggttatg
     9421 aagtgaaaat tgaaacccag ggcgcggacg gtatccagaa tcgcctgacg gcgcaggata
     9481 tcgccgaagc aaccatcatc atccactccg tggcagttac cccggaagat aatgagcgtt
     9541 tcgaatcacg cgacgtttat gaaatcactt tgcaggacgc aattaaaaac gctgcgggca
     9601 tcatcaaaga aatcgaagag atgattgcct ctgaacaaca gtaatattcg acagggataa
     9661 ttgttatggc cattaaaaaa cgcagtgcaa ccgttgtgcc tggcgcatcc ggtgcggcag
     9721 cggcagttaa gaatccgcag gcctctaaaa gcagcttctg gggtgaactc ccgcagcatg
     9781 tgatgtcagg gatttcacgc atggtgccaa ccttaattat gggcggtgtg atcctcgctt
     9841 tcagccagct gattgcttat agctggctta aaattcctgc tgagatcggc atcatggatg
     9901 ctcttaatag cgggaaattc tccggtttcg acctctcctt acttaaattt gcctggctgt
     9961 cgcagtcctt cggcggcgtg ttgtttggtt ttgccattcc gatgtttgct gcttttgtgg
    10021 cgaactctat cggtggcaaa ctggcgttcc cggctggttt tatcggcggg ttgatgtcta
    10081 cccagccgac gcaactgctg aacttcgatc ccagcactat gcaatgggcg acctcttcgc
    10141 cggtgccgtc aaccttcatt ggtgcgctga ttatttctat cgtcgcgggt taccttgtga
    10201 agtggatgaa ccagaaaatc cagttaccgg atttcctgtt agcgttcaaa accacatttt
    10261 tgctaccgat tctttccgcc atttttgtca tgctggcgat gtactacgtc atcaccccct
    10321 ttggtggctg gatcaacggc ggtatccgta ctgtgctgac tgccgcaggt gagaagggcg
    10381 cgctgatgta cgcaatgggg atcgccgcgg cgacagcaat cgaccttggt ggcccgatca
    10441 acaaagccgc aggtttcgtt gccttcagct ttaccactga ccacgtatta ccggtcaccg
    10501 ctcgttctat cgctatcgtt attcctccga ttggtctggg tctggcgacc attattgacc
    10561 gtcgtttaac cggcaaacgc ctgttcaacg ctcaacttta tccgcagggt aagaccgcga
    10621 tgttccttgc ctttatgggg attagtgagg gtgcgattcc gtttgcgctg gaaagccgca
    10681 tcaccgccat tccgtcgtat atggtcggcg cgattgtcgg ctcaaccgcc gctgtctggc
    10741 tgggcgcagt gcaatggttc ccggaatccg ctatctgggc atggccgctg gtcactaacc
    10801 tgggcgtcta tatggcgggg atcgcgctgg gggcagtcat aactgcgctg atggtggtgt
    10861 tcctgcgtct gatgatgttc cgtaaaggca aattgttaat cgatagcctg taagaaggaa
    10921 aatccagatg acattactcg cttcgctgcg cgactggctt aaggcgcaac aactggatgc
    10981 agtgcttctc tcctcacggc agaacaaaca gccgcatctg gggatctcca ccggacaggc
    11041 tatgtggtta ttagccgtga aagtgcgcac attctggtag attcgcgcta tttcgttgag
    11101 gtggaagccc gtgcgcaagg ctaccagctg catttgcttg acgcgacgaa cacgcttacc
    11161 actatcgtca atcaaatcat tgccgatgag cagttgcaaa cgctcggttt tgaaggccag
    11221 caggtgagtt gggaaaccgc gcaccgctgg aagtctgaac tcaatgcgaa actggttagc
    11281 gccacgccgg atgtgctgcg gcaaatcaaa acgccagagg aggtggagat aatccgcctc
    11341 gcctgtggga ttgccgatcg cggagcggag catattcgcc gctttattca ggcggggatg
    11401 agcgagcgcg agatagccgc tgaactggag tggtttatgc gccagcaggg cgcagaaaaa
    11461 gcctcttttg acaccattgt cgccagcggc tggcgcgggg cgctgccgca cggcaaagcc
    11521 agcgacaaga ttgttgcagc gggcgagttt gtcactctcg attttggtgc gctgtatcag
    11581 ggctactgct ctgatatgac gcgcaccttg ctggtgaatg gcgaaggggt gagcgccgaa
    11641 tctcacccgc tgtttaacgt ctaccagatt gtcctgcagg cacagctcgc agcaatctct
    11701 gcaattcgcc ccggcgtgcg ctgccagcag gttgacgatg ccgcgcgcca agtgattacc
    11761 gaggcaggtt ttagccacta tttcggtcat aacaccggtc atgctatcgg cattgaagtt
    11821 catgaagatc cgcgtttttc accgcgggac accacgacgc tacagccagg catgttactg
    11881 accgtggagc cggggattta tttgccaggg caagggggcg tgcgcatcga agatgttgtg
    11941 ctggttaccc cgcaaggcgc agaagtgctc tacgccatgc cgaaaacagt gttgctcacg
    12001 ggagaggcat aatggattta tcgctattaa aagcgttgag cgaggcagat gcgatcgcct
    12061 cctcggaaca ggaagtgcgg cagatcctgc tggaagaagc ggatcgcctg caaaaagaag
    12121 tgcgatttga tggtctggga tcggtgctga tccgcctcaa tgaatcgaca ggtccgaagg
    12181 tgatgatctg cgcccatatg gacgaagtgg gatttatggt gcgtagcatc tcccgcgaag
    12241 gggcaattga tgtgctgccg gttggcaacg tgcgcatggc tgcccgccag ctgcagccgg
    12301 tgcgcatcac cacccgtgaa gagtgcaaaa ttccaggcct gcttgacggc gaccggcagg
    12361 ggaatgacgt cagcgccatg cgcgtggaca ttggtgcgcg ctcgtatgac gaagtgatgc
    12421 aggcgggaat tcgtccaggc gatcgcgtca cctttgatac aacttttcag gttctccctc
    12481 accagcgggt gatggggaaa gcctttgatg atcgcctcgg ttgctatctg ctggtgacgt
    12541 tactacgtga gctgcacgat gccgaactgc ctgcggaggt gtggctggtg gcaagttcca
    12601 gcgaagaggt gggattacgc ggtgggcaaa ccgctacccg cgcggtgtcg ccggacgtcg
    12661 ccattgtgct ggataccgcc tgctgggcga aaaactttga ttatggcgcg gctaaccatc
    12721 gccagattgg taacggcccg atgctggtgt taagcgacaa gtcactgatt gcgccgccaa
    12781 aacttaccgc ctggatcgaa accgtggcgg ctgaaattgg cgtaccgttg caggcagata
    12841 tgttcagcaa cggcggcacg gacggcggag cggtgcactt aaccggtact ggcgtaccca
    12901 cagtggtaat ggggcctgcc acccgccacg gacattgcgc ggcatcgatt gccgattgcc
    12961 gcgacatttt gcagatggag caacttttat ctgcccttat tcaacgtctt acgcgtgaga
    13021 cggttgttca actgacggat ttcagatgat ctcctgatta acccggagcg gttatgttaa
    13081 cgattcaatt tctctgtcct ttgcctaacg gtctacatgc tcgtccggcg tgggaactta
    13141 aagaacagtg cagccagtgg caaagcgaaa tcaccttttt caaccatcgc cagaacgcaa
    13201 aggccgatgc taaaagctcg ctggcgttga ttggaactgg caccttattt aatgacagtt
    13261 gcagcctgaa cattagcggc agcgatgaag agcaggcgcg gcgcgtgctg gaagagtaca
    13321 tcctggtgcg ctttatcgac agcgacagcg ttcagccgac gcaggcagaa ctgacggcgc
    13381 atccgctgcc gcgttcatta agccgcctta acccggattt actgtacggc aatgtgctgg
    13441 caagcggcgt cggcgtgggt acgctgaccc tgttacaaag cgacagcctc gacagttatc
    13501 gggcaatccc cgccagtgcg caagattcca cccggctgga gcacagcctg gcaacgcttg
    13561 ccgagcaact gaaccagcaa ttgcgtgagc gtgacggcga aagcaaaact atcctcagcg
    13621 cccatttgtc gctgattcag gatgatgaat tcgcgggcaa tatccgtcgt ctgatgacag
    13681 aacagcatca agggctgggg gcggcgatca tcagcaatat ggagcaggtt tgcgccaaac
    13741 tttctgcttc tgccagtgat tatctgcgcg aacgtgttag cgacattcgc gatattagcg
    13801 aacagttgct gcatatcacc tggccggaac tgaagccgcg caacaatctg gtgctggaaa
    13861 aaccgactat tctggtagcc gaggatttaa ccccaagcca gtttttgagc ctcgatttaa
    13921 aaaatcttgc aggcatgatt ctggagaaaa ctgggcgcac ctcgcatacc ctgattctgg
    13981 cccgcgcctc ggcgatcccg gtactgagcg gcctgccgct ggatgcgatt gcccgttatg
    14041 ccgggcaacc ggcggtgctt gacgcccagt gcggcgtgct ggcgattaac ccgaatgacg
    14101 cggtgagcgg ttattatcag gtcgcacaga cgctggcgga taaacgccaa aaacaacagg
    14161 cgcaggccgc cgcgcagctg gcctattccc gtgataacaa gcgtattgat attgcggcga
    14221 atatcggcac cgctctggaa gcgccaggcg cgtttgccaa cggcgcggaa ggcgttggac
    14281 tgttccgtac tgaaatgctg tatatggatc gtgacagcgc gccggacgag caggagcaat
    14341 ttgaagccta ccagcaggta ctactggcag cgggcgacaa gccgattatc ttccgcacaa
    14401 tggacatcgg tggcgataaa agcattcctt atctgaatat tccccaggaa gagaacccgt
    14461 tcctcggcta tcgcgcggta cgtatttacc cggaatttgc tggcctgttc cgcactcaac
    14521 tacgggcgat tttgcgcgcc gccagttttg gcaacgccca gttgatgatc ccgatggtcc
    14581 acagtctcga tcagatctta tgggtgaagg gcgagatcca aaaagcgatc gttgagctta
    14641 agcgcgatgg cctgcgtcat gcagagacga ttacgctggg gatcatggtg gaagtgccgt
    14701 cggtgtgcta catcatcgat cacttctgcg atgaggtcga tttcttcagt atcggctcca
    14761 acgatatgac ccagtatctg tatgcggtcg atcgtaataa cccgcgcgta tcgccgctgt
    14821 ataacccgat tacgccatcg ttcctgcgca tgttgcagca gattgttacc actgcgcatc
    14881 agcggggcaa atgggtaggc atttgcggtg aactgggcgg tgaaagccgt tatctgccgc
    14941 tactgcttgg gctgggcctg gatgagctga gtatgagtag cccgcgtatt ccggcggtga
    15001 aaagccagct tcgtcaactg gatagcgagg cgtgtcggga actggcgcgt caggcatgtg
    15061 aatgccgcag tgcgcaggaa attgaagcgt tactcaccgc ctttacgccg gaagaagacg
    15121 ttcgcccact gctggcgctg gagaatatct ttgttgatca ggattttagc aataaagagc
    15181 aggcgatcca gttcctgtgc ggcaacctcg gcgttaacgg gcgcactgaa catccgtttg
    15241 agctggaaga agatgtctgg cagcgggaag agattgtcac taccggcgtt ggttttggcg
    15301 tggcgatccc gcacaccaaa tctcagtgga tccgtcattc cagtatcagc attgcccggc
    15361 tggtgaaacc ggttgactgg cagtcagaga tgggcgaagt cgaactggtg atcatgctga
    15421 cgttgggtgc taacgaaggg atgaaccatg tgaaagtctt ctcgcagctg gcgcgtaaac
    15481 tggtgaataa aaacttccgc cagtcattgt ttgccgcgca agatgcgcaa agcatcctga
    15541 cgctgctgga aacggaatta accttctgac gttagccctg aaaacgggcg ctgtactctc
    15601 ccggcgtcag accaaactga cgccggaaaa cgcgacaaaa atagtcgctg tccggaaaac
    15661 cgcaacgctg tgccacttca tgaattgaca gatgatattt ctgcaaaatc atcctcgcct
    15721 tcgccattcg cacccaacgc acatactcga taaaacgcat cgttccatgc tgagcaaaca
    15781 gttttgacag atgattgggc gtgatattaa aaaactgcgc cacgctctcg cgggtgagcg
    15841 gctgggcata attatcctga acccagttac aaatgctgtg atagagaaaa tcgccgcgcg
    15901 gttgcgaatt acccggcggc atattcacca cgttgcggca aagatgcagc aaacttaaca
    15961 ccagtggctg aatgatattc tgctccagcg gcgaacggct aagatgggtg agggcggtaa
    16021 gcatcgcttc accttcaccg cgttgcaggc tgggaagctg gatctggcga gcaggatgca
    16081 acaacgacgt ggtgcggcta tcgtaaaacg ataacccaag ccaggtcggt gcgaacacca
    16141 ggcttaacag catcaccggt ttgttgttga tcggtaaatt agccgcgcgt gccggaataa
    16201 acagcatttc tccttccacc attcgctgct gctttgcttc cagcaggttg ccgtattcgc
    16261 cgcgcagcac gatatccaga cggggaaaat caatacataa actgcccacc ggtaacgagg
    16321 caggctggct ggcaaaccag acgcgcccca gtagttgcgg gttaagcacc aggccgctga
    16381 acagatcggc gaaaaattgt tgatcggcgg gcaagcctgg tgctttcatg aaagttcccg
    16441 attaaagatg cattaactgg cgaaattctt tcactttgct gcggctgacc ggcacttcaa
    16501 aatccagatc tttcagtcgc agaatgtagg tgttattaaa ccacggttcg atttcgcgta
    16561 ttttgttcag attgacacaa aatgagcgat ggcagcggaa aaaatgcgac ggcggcagtt
    16621 tgctgcaaaa ttcggtaatg ttcatcggca ttacgtagga ttcacgccgc gtatagacaa
    16681 aggtcatttt ctcgtgcgct tcggcgtaat agatatcgtt aattggcgtg acgattattc
    16741 gctcatcttt caccagatta atggtgtcat tctcacgcgt taccgtcgcg gcaggcgtac
    16801 tgctggtctg ctgttgttgc caggccgctt ccagtttttg cagcatcccg gtaatacgtg
    16861 actcctgata cggtttgaga atgtagtcaa acgcctccag ttcaaacgct tctaccgcat
    16921 gttctttcca cgcggtgatg aacacaataa acggtttatg ggcgaactgg ctgatgtttt
    16981 gcgccagcaa cacgccatcc agcgacggaa tattgatatc cagaaaaatg gcgtcgacgc
    17041 ggttatgctg caaaaacttc aacacgtcca gaccgtcgtc aaaggtgccg acaatctcca
    17101 tctggctgtg ctctttaatt agccagctca gttcctgttg tgccaggaat tcgtcttcaa
    17161 caatgatgac tttcacaata tcactccggc tcaaagcaat aacgtagcct gtgaggcgac
    17221 tggggtgcgt tggttaggaa tgtaaaacgc aatttccgtc cccggctcca ggcggcggat
    17281 atgcagcccc tcgccataca ataacttcac gcgatgatgg acattcagca ggccaatttt
    17341 attgcccggc atttcattcg cttcgacccg ctcaatcacc tttggatcga tgccgtgccc
    17401 ggtatctcgc accgcaatgc gtacccgatt tccgcactct gcaacgctga tggtgacaac
    17461 gcctttacct ttgcaaggct gaataccgtg gacaatggcg ttctccacca acggctggat
    17521 cagcaggctg ggaatgcagc aattcacctc ttcatcaata tcatagataa ccgtcagctt
    17581 gtcaccaaag cgggcctgct caatggcaat atagtcttta atttgataca gctctttttt
    17641 gatatcgatt tgctcatcgt cttttaattc aatgttatag cgcagataac gcgacagatt
    17701 aaagatcaac tggcgagcgg tatccggatt cagacggatt gacgatgaaa tagcgttcag
    17761 agcgttaaac agaaaatggg gattaatttt gctttgcagg gcgcgaagct ctgccttatt
    17821 tgccatttca cgtagctgct cggcgcgtga aacctccagt tgcgtggaga tgatttgcga
    17881 cagaccgacc gccatttcct gtaatgacga ggtgatctga tgcgcgtggc agtagtaaat
    17941 tttcagcgtt ccggtcacga cccctttctc ccacaatggg atcaccagca tggaatgaat
    18001 ctctggtgtg cggtgggctt catcattgtt tttaatgatg atttttccgt aattcatcgc
    18061 ctgacgggta gtcgggctaa tgaagtcatc gccattctga tagttatgtt cacccacgcc
    18121 aacataggcc agcacatgat cggtattggt aattgccacc gcatcggcgt gaatgtcatc
    18181 gcggataatt tcacagacct tgcgtaatga ctcgctattg acatggcgaa acagcggtag
    18241 cgttttgttg gcgatatcca gcgccagctt ggcctgccgc gcggcgctgg cctctttttc
    18301 gccctcaacg ctttgcacca gaagcacaat aaagccgata cagacgctac cgaggatcat
    18361 tggaatgccg attttagaga cgatatcgat ccccaacgcg gtagttggtg cccagacaat
    18421 taccagaatc atggtcaacg tctcacacaa catgccgccg agaataccga cgcgccagcg
    18481 ctgtgctttg gggattttca ggttgatcca gcccgatata caacccgcca gaatgctggt
    18541 gataaagcag gggatcgccg tcacgccgcc gatatcaatt aaataccggt gaatacccgc
    18601 aatcacgccg gtaatgatgc ctacccacgg gccaaacaga atcccgccgg acatcaccgc
    18661 gataatacgt acgttcacca gcgagccttc tacgggaacg ccggaccagg tgctgaacag
    18721 ggcgaacagc gagaaaatgg cggtaacggc gagcaattct tttggggagt gcgccgactt
    18781 gtgcaacagt tcgcgaaaca ggcggatacg gatgagaaag aacaggcaga taagcattaa
    18841 cgctgcccga tcgaagaccg ccagcagcat gttgaatatt tcgtgcacgg gtgactctga
    18901 ataaggatta aacctctatg ataggtaacc tgaaggctga tgaccagcag gccgtttttg
    18961 aggaaaaacc ataaatttaa aaggggattg
//
LOCUS       DAFNBJ010000093        18978 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000093 DAFNBJ010000000
VERSION     DAFNBJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.093"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..103)
                     /locus_tag="L2X49_003080"
     CDS             complement(<1..103)
                     /locus_tag="L2X49_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012472779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBN4798123.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEM"
     gene            complement(296..1447)
                     /gene="nhaA"
                     /locus_tag="L2X49_003081"
     CDS             complement(296..1447)
                     /gene="nhaA"
                     /locus_tag="L2X49_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005393226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBN4798124.1"
                     /translation="MNDVIRDFFKMESAGGILLVIAAAIAMTIANSPLGETYQSVLHT
                     YVFGMSVSHWINDGLMAVFFLLIGLEVKRELLEGALKSKETAIFPAIAAVGGILAPAL
                     IYVAFNANDPEAISGWAIPAATDIAFALGIMALLGKRVPVSLKVFLLAVAIIDDLGVV
                     VIIALFYTGDLSTMALLVGFIMTGVLFMLNAKEVTKLTPYMIVGAILWFAVLKSGVHA
                     TLAGVVIGFAIPLKGKQGEHSPLKHMEHALHPYVAFGILPLFAFANAGISLEGVSMSG
                     LTSMLPLGIALGLLVGKPLGIFTFSWAAVKMGVAKLPEGVNFKHIFAVSVLCGIGFTM
                     SIFISSLAFGNVSPEFDTYARLGILMGSTTAALLGYALLHFSLPKKAQA"
     gene            complement(1608..1793)
                     /locus_tag="L2X49_003082"
                     /pseudo
     CDS             complement(1608..1793)
                     /locus_tag="L2X49_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1801..2871)
                     /locus_tag="L2X49_003083"
     CDS             complement(1801..2871)
                     /locus_tag="L2X49_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBN4798125.1"
                     /translation="MQNNFQSPESVILGVDTHLDIHVGAVISQAGKLLGTRIIQTNQI
                     GYQELFDWAKSFGVLERAGIEGTGTYGAALTRFLIKNSIYVVEVNRPDRSKRRLEGKS
                     DPLDAENAARTVLSGSSKAIPKMQSGACEALRIISVARRSAVKARTQAINQLRSLLVS
                     APQDVRDKLWKSKPHECVAACINCEVSEYSPLRIALYSTLRSLAKRWGALTEELNELD
                     ESLDKLTRKYASNLRSQFGVGPQTAATLLAVAGDNPERLKNEAALASLCGVNPLPASS
                     GKTVRHRLNRGGSREANNALWTIALVRMRSDPRTRIYVDRRTGEGLSTKEILRCLKRY
                     IVRELYPLILSDLTYANRPLLT"
     gene            complement(3192..4268)
                     /locus_tag="L2X49_003084"
     CDS             complement(3192..4268)
                     /locus_tag="L2X49_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBN4798126.1"
                     /translation="MVDKVTEAAVVGGVDTHKDLHVAAVVDQNNKVLGTQYFSTTRQG
                     YRQMLAWMTSFGTLKRIGVECTGTYGSGLLRYFQNAGLEVLEVTAPDRMERRKRGKSD
                     TIDAECAAHAAFSGIRTVTPKTRDGMIESLRVLKTCRKTAISARRVALQIIHSNIISA
                     PDELREQLRNMTRMQLIRTLGSWRPDASEYRNVTNVYRISLKSLARRYLELHDEIADL
                     DVMIAAIVDELAPELIKRNAIGYESASQLLITAGDNPQRLRSESGFAALCGVSPVPVS
                     SGKTNRYRLNRGGDRAANSALHIIAIGRLRTDAKTKEYVARRVAEGHTKMEAIRCLKR
                     YISREVYTLLRNQNRRINSIPITA"
     gene            complement(4550..4867)
                     /locus_tag="L2X49_003085"
                     /pseudo
     CDS             complement(4550..4867)
                     /locus_tag="L2X49_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963400.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5045..5479
                     /gene="umuD"
                     /locus_tag="L2X49_003086"
     CDS             5045..5479
                     /gene="umuD"
                     /locus_tag="L2X49_003086"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBN4798127.1"
                     /translation="MSVFPERFASPPRDTTPFFSDLLSCGVMSPCAGHEDNELNLHDF
                     VVRNRPATFFVRASGLSMVDAGINDGAILVVDRSLTAEHGSIVVALVDGEFTVKILQT
                     FPKLMLMPSNSAFKPIPVNPDTLEIWGVVTFALNQFPHVHAR"
     gene            5463..6722
                     /locus_tag="L2X49_003087"
     CDS             5463..6722
                     /locus_tag="L2X49_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBN4798128.1"
                     /translation="MYMHVDINGAYAAFECAMDPKLAKSPLIIASNNDATIIAMNRLA
                     KNTGLKRGVPIFKCRDLIEKHHIAVRSSNFTLYEDYSNRFHETLEGFAEDTDRYSVDE
                     SFMLLKNMDKIVDFEEYGCLIRRTLLHNLSLTCGIGVSTTKTLAKLCTYASKRWAATG
                     GVVVLTDPFRIKKLLSLISTREIWGIGRKISERLAAYGIITACDFYDSDVRFLRKAFG
                     VEMERTWRELHGESCFRLHESPPVRQQIIVSRSFGQRLNTFSKMHEAVSFFTGRAAEQ
                     LRNDGSWTRQITVFIQSSPFARAETRYSSCGIEPLTATQDTRDLVSAAGAILTRIYKP
                     GIDYAKAGVMLSSMTDGTEQLSLFDERPARRGSEALMKVMDRFNKEHRGSLFLACEGI
                     HQDFRGKQAMLSPRYTTRWNELLVVKA"
     gene            complement(6774..7583)
                     /locus_tag="L2X49_003088"
     CDS             complement(6774..7583)
                     /locus_tag="L2X49_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBN4798129.1"
                     /translation="MTKLNYLKPVASVLISAVIATAGSVYITSKYLKPDVPGNDEIGR
                     VAASYLVKNPQYLLEAGKALEKENTNASLERIIPYAPALFETKETPNIGPDNAAVAVI
                     EFFDYQCHFCMQVAPVVESVLSQSTDVKFFFKEFPIFAGSKPVSAMGAATGLHVYQTF
                     GAEAYRKYHNNLMTSAYVFFNNQRAFTLNDLDMVVNKSGFNSSFGDREKSRYENVISG
                     NMQLGEALGINGTPGFIIMNMQKPDAATTSFIPGAVDEATLKYAIQKARGG"
     gene            7690..8301
                     /locus_tag="L2X49_003089"
     CDS             7690..8301
                     /locus_tag="L2X49_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBN4798130.1"
                     /translation="MLRQFFLLTATTFLCGCGYHFGNDVDAYDLLPRPVGSKKFEIVA
                     PDDSIQSRMFAARFASGLTGKGFNISSHQPEYILRFSYSKTQENLQYSELPVTGITGY
                     VIEKKTTRKDKHGQTETDYDYKPVSGIVGTETVSQRHFWRRLDVEVYPAGKNAQQVLK
                     VSMQSNAPIPSDSVAYSAMIDALTGNLDAPLRSGNYVASVPWN"
     gene            complement(8360..8782)
                     /locus_tag="L2X49_003090"
     CDS             complement(8360..8782)
                     /locus_tag="L2X49_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798131.1"
                     /translation="MPLWLSIVTRATGLPDKRYLNDPVILRYYQQKIQKAPYALVGWT
                     LCFMATIGVSLGLDLSNQMLLLVLLILIFLMVVLQGRYQFMHPERCINAKFYQTVLDD
                     ASVSDIQKAQFEYLFVTKVDELTIGDLYRLYDFNRGGR"
     gene            complement(8805..9197)
                     /locus_tag="L2X49_003091"
     CDS             complement(8805..9197)
                     /locus_tag="L2X49_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798132.1"
                     /translation="MKSAILTTLMLVAVGSQPVFAAKCQFGACDADTVASSEILVSAA
                     QTDKDAVLKKLNDATSRSDVMSKRFESYLSNDRLKVNTDSTKKGPSNEIAKPRDELAP
                     VRQLKAIRPELVQTPIFQPIVAFYERLA"
     gene            complement(9223..9972)
                     /locus_tag="L2X49_003092"
     CDS             complement(9223..9972)
                     /locus_tag="L2X49_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HBN4798133.1"
                     /translation="MNKTIKASLFLLASCMAFSMNAQTANTDVLPDAALKKLREVGLS
                     IEHIEPSPVKDIYTVISREGVSYVSKDGDYIFTGSLFHVNGKDVENTTEQAILKGVRE
                     FATKTKSIEYKSANEKYRLAVFTDITCGFCQKLHQDLQSYLDAGISIKFLAFPRAGLN
                     SVVAGNMAKIWCAAKPNDALDAAMSPTSTIPEGRPDEACLDTIKSHFQVASTIPLQGT
                     PTMVTLSGKPQLFTGWMSPENLVTRMGAEQK"
     gene            10120..10635
                     /locus_tag="L2X49_003093"
     CDS             10120..10635
                     /locus_tag="L2X49_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBN4798134.1"
                     /translation="MFIPPVDDVKPIPVPVEIYTQCITDASRFFGIDAELVFTLFDNE
                     GGKVGTFSRNKNGTYDIGPMQINSSNLPEIRDHFPSVTWRVLAYDACASFWVGTWWLY
                     RKIVDRKGNVFEGIADYNSKTPKVRATYIFNFMIKYNRRIQRRNGMDELYQWTQPKPQ
                     YNGHIVKNLPE"
     gene            10732..11298
                     /locus_tag="L2X49_003094"
     CDS             10732..11298
                     /locus_tag="L2X49_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798135.1"
                     /translation="MLQSIYKFLFNLCSSPQQPKNKDVDGILSHEEITAGLWGPLPSI
                     HVVLFLDFDGVVHKCQNESFERMHLIERLLDASPSMFIVISSSWRECGSITYLKSLFR
                     SPYRDRVIGATPSLYLPSGSIGVRAAECEDFVTRHKVKAFICLDDDATLFPPEYPHLF
                     RTDYYTGVNEKDLTDLIARYNVLMTRFY"
     gene            complement(11545..15534)
                     /locus_tag="L2X49_003095"
     CDS             complement(11545..15534)
                     /locus_tag="L2X49_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HBN4798136.1"
                     /translation="MDYNIYTVGDIEFVWSALNGIALIFSQYTGVKAFLTTAAVLAGA
                     SLFYKTWLWLLNPTKAEVPIFSWILGLILFSMAIVRVDVTIESVKSGEVRNVDGIPIF
                     IAAMGTVTTNLSQGLLKDYKTAFDPLAPIDFAATTLDDDITLGPMIRFVKFLQWGGDS
                     QGYCSAFPEPASGLGAMNVCATVQSLAYNCLKGTQNSSAKIAGKETIFNDIFSANIVD
                     SMERINQAMKGSLKNASANIVGVNSSKSATCEEVWSTVKQVTSTPEARQTIALIGQTN
                     GILAPDEAGGAASGSSFTDVMASANGMYGKAIGAYDATLSLFIMNELRNGASKYKTPL
                     GLASDMQLFEASLKRTNTMASQGQLWLQLSGAAIAFLEMFAYMVAPFALLMLLALGGN
                     GVAAAAKYLQLILFVNMWPITAVMVNAYVKKVATADLDTWSTLNSQSNAVTWMGLPGL
                     AETYSSYLSVASALYALIPVLTLFLMTQSIHPMMNAMKGVTPPAPVDSSHLTPKVWDA
                     PNSGKSSFGDVNRTALTSTGQGFSGGGAMDSSNFRLGTWNAGSSIASSQGQGAAFTSS
                     AMSAASNTFQQAFNQMSEVGRTGQSGQQFTSNLQSLKSFTDQAGANIANSVGAKHGIN
                     SQQLAGVVSNLLLNGSLGLGGEEKLGKGGGGGLAQKAMDSLGIKAGGQVGSGITKSNS
                     GNESLLTDLSKAITTQLTENSGLTDQMTKAAAQINSDQFAQTNAFKEAATKMNQSTQT
                     MAENLSTSLSTNASANTGMTLDSRQAINLDRFSDSIRNKNFSDEDVRNFARKNGLDEN
                     AFMEKFNSYNDTFKASTQLGSQLQRTDALVATTRDFSEQKIAIDTARGETAESNKQDL
                     RETSSLLKGLVADFGGNAQQMLPITNQLDRITGDASGINTIAQAQDRTPEHVNTSSVM
                     SADRVADVGQSMDAQAKAGLVQNQQNANQHVGGQTESGVTPYNVDKVSKEDIQGIHQG
                     NVNKSYSEENQNVLSSLDKNTPVVTNETVGRVQGAGVDVQHANQTFDQLQAGSGGKHA
                     TSGMGTTSDKLNNMYGDNQIRGISGNLPNYFDRIANDPTKKPDDKRAELAAQAVFTYG
                     ASTMATGAEKEQLKADTQNILSELSHYNVRWGMNDLQNIHEGFNMHNKASGSLDSVVR
                     ANLGEGGSGGGLVGQRTQTTTDLLVGNKIESNTERGVIGSTLLSGQESVARGLDVIGA
                     KPVNDMLSGMGILTTPSSLANDASNPQNMPSSLLGKVGNHLQMSDGVSAVTSRYKDMG
                     GDGIKAYETASQNSERAIRQQLTDDPRFGPDKADAFVSFMKSNLNNTNEPYQSRIEKA
                     ESWLNENKK"
     gene            complement(15543..16958)
                     /gene="trhH"
                     /locus_tag="L2X49_003096"
     CDS             complement(15543..16958)
                     /gene="trhH"
                     /locus_tag="L2X49_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhH"
                     /protein_id="HBN4798137.1"
                     /translation="MRADLHVCAKKLLLSLAVCTAMIAPNAVADNAMRNIFNGMMTTT
                     SPASFETATRTGVVGGSFSYRTTNVNTNLVSMSFPKASVGCNGIDVFLGSFSMINGDQ
                     LVQVARGIAQGAAIYAFNVAVSAICADCAATINDIQNKLQALNKFAKDSCNATYSFLS
                     ETNGPPSQFANAVSSGPASVLGSLNGLLSDFGSSMTKAPETVSAQVKAKDPEGFAEKF
                     SGNLFYMSFMDIDKGSMNIGGVTELSGYKLAEQLMSLVGTVIINWDAKGEKAGMEVRP
                     STMTVSDFIMGPPAGGSIKMLKCSPAPDPASARKSQCLVMSEVTDGGFKGLKDTISDL
                     LIQVQTKAISDVRISDDELRIISYIGIPTILDSLQTFDVPEGYAYIQDISAIAATSLV
                     INMLRQVEAKISAMNIPSESLSGRRQDLIRLSENLTNQVKAAYELTRSQVGNSSDVIS
                     AWDDRRLKRKAFMESLRGTGN"
     gene            complement(16948..17994)
                     /locus_tag="L2X49_003097"
     CDS             complement(16948..17994)
                     /locus_tag="L2X49_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBN4798138.1"
                     /translation="MTRLTLIPSKMLVLLSLFITGVHANEEQALIKDTPFVSGQAFKK
                     GFFWYDDPTRKTEEEITETKPPVASSTQPTQEEKIELNSKWLKDNMPQLLTKAMDNPT
                     PENLSRFYTAQRLMLDIGTRFSDKSKDYFLKNPMMSEKRRQPVEKVALDAHRTVVEKN
                     QQSVMKDIFTKSGLFFFFQSTCQFCHEESQILQFMENYYSVDILPVSMDGRPLQNGLF
                     QDFSVPNAQIIDQFKIREVPTIFLVSKDGSSAQRISEGMITAEELKNTIILAAKGMKL
                     IDDASFQSTLDIKRQYTIGEDGVITVNKSEMDSDPFLLQRIMDKKLEGYDMPTADPVN
                     YLNVGGSLGGAYAR"
BASE COUNT         4932 a         4649 c         4359 g         5038 t
ORIGIN      
        1 gcatctcctg cagctcacgg taactgatgc cgtatttgca gtaccagcgt acggcccaca
       61 gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc tccatcagca
      121 aaaggggatg ataagtttat caccaccgac tatttgcaac agtgcccaaa ataccctcag
      181 caattagtgt actgagggta ttgttaacca tccgtaggat cgttcaactg atcaatgcct
      241 taactgatcg gtgttatccc gattgggagg ctttacaaaa tagctcattt ttatcttacg
      301 cttgcgcttt cttaggcaaa gaaaagtgca gtaaggcata gccaagtaat gctgctgtcg
      361 tagaacccat taagataccg agtcgagcat aggtatcaaa ttcaggactt acatttccga
      421 acgctaaaga ggaaatgaag atagacattg taaagccaat accacacaac acagataccg
      481 caaagatatg cttgaagtta acgccttcag gtaacttagc aacgcccatt tttaccgctg
      541 cccagctaaa ggtaaagata cctagaggct taccaaccag tagacccaaa gcaataccga
      601 gtggcagcat tgaagttaga cctgacatcg atacgccttc taacgaaatc cccgcattgg
      661 caaatgcgaa tagaggcaga ataccaaatg ccacgtatgg gtgaagcgcg tgctccatat
      721 gtttaagtgg agaatgttcg ccctgctttc cctttagtgg gatagcaaaa ccaatcacta
      781 cacctgccaa tgttgcgtga acaccagatt taagcaccgc gaaccataag atcgcaccga
      841 cgatcatgta tggcgtaagt tttgttactt ccttcgcatt caacatgaat aggacaccag
      901 tcatgatgaa gccaacaagc aacgccatgg tcgacaggtc accggtgtaa aatagcgcga
      961 taataacaac tacacctaaa tcatcgatga tagcaaccgc cagcaagaac acttttaggc
     1021 ttactggtac acgcttacct aataacgcca tgatacctaa agcaaaagcg atatctgtcg
     1081 ccgctggaat tgcccaacca gaaatcgcct caggatcgtt ggcattaaag gctacgtaaa
     1141 taagagcagg agctaatata ccgccaaccg ctgcaatcgc agggaaaatt gcggtttctt
     1201 ttgattttaa cgcaccttca agcagctctc gcttaacttc aaggccaatg agtaagaaga
     1261 aaacagccat taggccgtca ttaatccagt gagaaactga cataccaaat acataagtat
     1321 gcagtacaga ttgataagtt tcgcccagcg gtgagttagc aatggtcatc gcaattgccg
     1381 cggcaatcac gagaagaata ccaccagctg attccatttt gaaaaagtca cgaatgacat
     1441 cgttcatggt ttcgtcctta tatttattat cttaataaac gattaacaaa gaatgataag
     1501 agtgtatagc ggtcgaaaac tttagaataa ttgaatcgcc acggataatc tagacacttc
     1561 cgagccgttg attggactga ccccacgccc gtagacaaat tctgtcctca cgacgaggcc
     1621 tgttcgaagg cctccggact gacgccgccg aggtgactgt ggcgccgggc ccggttgtag
     1681 aacacttcaa tgtaatcgaa gatatccgcc cgggctagat cccgggtttt gtatattctc
     1741 ttcctgatgc gctctttttt cagtgaactg aagaacgatt cggccaccgc attgacgctc
     1801 ctatgtcaag agcgggcgat tggcatatgt gagatctgat aaaatcaatg ggtagagttc
     1861 acggacgatg tagcgtttta gacatctcag gatctctttg gtcgatagcc cttcaccagt
     1921 tcgtctgtcg acatagattc ttgtccgcgg atcacttcgc atacgcacaa gggctattgt
     1981 ccacagggca ttgtttgctt cacgtgagcc acctcgattt aatctgtgcc tgactgtttt
     2041 tcctgatgat gctggcagcg gatttacgcc acaaagcgat gccagtgctg cttcattttt
     2101 taaacgttcg ggattatcac ctgcaactgc cagcagtgtg gctgccgtct gtggccctac
     2161 gccaaactgg ctacgaaggt tactggcata tttacgggtc agtttatcca gcgattcgtc
     2221 cagttcattc aactcttcag tcagtgcccc ccatcgtttc gcaaggcttc taagcgtgct
     2281 gtacagagct atacgcagtg gagagtactc tgatacttcg cagttaatac aggcagctac
     2341 gcactcgtgt ggtttcgatt tccagagttt gtcgcggaca tcctgcggtg cactaaccag
     2401 cagcgaacga agctggttaa tcgcctgagt acgggctttt acagcgctac gtcgggcaac
     2461 gctaattatc cggagagctt cacacgcgcc ggattgcatt ttgggtatgg ctttgctgct
     2521 ccccgaaagt acggtgcggg ctgcattctc tgcatccagt ggatctgact ttccttcaag
     2581 gcggcgtttt gagcggtctg gtcggttaac ttcaacaaca taaatactat ttttaataag
     2641 aaaacgtgtt aatgcagcac cataagttcc ggttccttca atacctgctc gttcgagaac
     2701 tccaaaagat ttagcccaat caaatagctc ctgatagcca atttgatttg tttgaataat
     2761 gcgagtacca agcagcttac ccgcctgact gataacagca ccgacatgaa tatcaaggtg
     2821 tgtatcgaca cccaggatca cgctttcagg agattgaaaa ttgttttgca tggtttaccc
     2881 cccagatatc agtagcacaa ccatccccat cgcaggacag gacactcaag atgcagcaca
     2941 aagctcctat caggtcacaa acgctgggcc cggctatgcc cgggaaatac cagaatcgga
     3001 cgacaggtca acacaaaggc attcgagcca atcccagcac gggtcagtcc gatccggtat
     3061 ttgaaagtat attagaatga gtatcccagc agttgccgcg acggctcatg ctcggtacca
     3121 gattattggc ccggcagaac cgctgccagt catcactgcc atactgactg ccctggacgc
     3181 ccttctaaga gtcaagctgt tatcgggata ctgttgatcc gcctgttttg attgcgcagt
     3241 aacgtgtaaa cttcgcggga gatatatcgc tttagacagc gtatcgcttc catttttgta
     3301 tgtccttcgg ctacacgcct ggcgacatat tcctttgttt ttgcgtcagt tcgtaagcgt
     3361 ccgatggcga tgatgtgaag tgcactattt gcagcacgat ctccaccacg attaagtcgg
     3421 taacggttcg tttttccaga agaaacgggg accgggctga caccacacag tgccgcaaat
     3481 cctgattctg atcttaaccg ttgaggattg tctccggcag tgatcagcaa ctgtgaagcg
     3541 ctttcgtatc cgatagcatt acgtttaatc agctcaggtg ccaactcgtc gacaattgcc
     3601 gcaatcatga catccagatc agcgatttcg tcatgtaact cgagatagcg tcgggcaagg
     3661 gactttaatg aaatgcgata aacgttggtt acattgcggt attcactggc atcaggtctc
     3721 caggaaccca gggtcctgat gagctgcatg cgcgtcatat ttctgagctg ttcacgtaat
     3781 tcatccgggg cagagataat atttgaatgg ataatctgga gagcgactct gcgggctgat
     3841 attgctgttt ttcggcaagt ttttaatacc cgcagggact caatcatgcc atcacgcgtt
     3901 ttgggagtaa ctgtcctgat gcctgagaat gctgcatgag cggcacattc agcatcaatt
     3961 gtgtcacttt taccccgttt gcgtcgctcc atccggtctg gagcagtcac ctcaagaact
     4021 tctaacccgg cgttctgaaa ataacgaagc aaaccggaac cataggtacc tgtgcactca
     4081 acaccaattc gctttaatgt cccaaacgag gtcatccatg ccagcatctg ccggtaacct
     4141 tgtcgtgttg tggagaaata ctgagtccca aggactttat tgttctgatc tacgacagcg
     4201 gcaacgtgca aatctttatg tgtatccacg ccacccacaa cggcagcttc ggtaacttta
     4261 tcaaccatga cagagctcct gtgaggaaac agagttgaat ctccagacag ataacccgga
     4321 caggacagta acgagacaag ccgtcaggcc cttcttgagt cacgcgcatc ggtgaggaga
     4381 tgcctcgcat gaaggcgctt ccggcaaccg acgggtccag ggcaggacac aaaaggtcga
     4441 tcgctgtgtg agtcaggatg cgggaaggtc ttcactgcat cagtaatcac accaatctgg
     4501 ttaaacggca aactaagtgg tggtaaagac tgacatcggc attattactg tgagcccttc
     4561 ttagcagagc attactgagt gtggccatat cgcttaagtc caccagcggt gttggtgcct
     4621 gcggttgtgc ctgcgacgcc ttcagcttca tcgtcaccct cagtgaggtc tcaatcccgt
     4681 tcaggaaagt cgtacaggcg gctagccaca actcaccgtg aatggcccgg gtagcctggt
     4741 caaagaacca gtcaatcgca gctgtcgttc ggtacgccct gtatgcctcc agttcccgta
     4801 actgtgccag gtacttctcg cacgcctctg cgtctaccgg atcgttatcg tcggtatcga
     4861 tagccatagg gaattcctgt tgtcatgttc gtgaatgaaa atccgctgaa ccatatcata
     4921 ccgttacata aattacggct tagtgccggg atatctttcc cgaatgctga gccattgaaa
     4981 tttgatcttt tctctttatc tggtgatact ggttaaatat ccagtattga atccgagtgt
     5041 gctcatgtct gtattccctg aacgttttgc atcccctcct cgtgatacaa cccctttttt
     5101 cagtgaccta ctgagttgcg gggtgatgag tccgtgcgcc ggacacgaag acaatgagct
     5161 gaatttacat gattttgtgg ttcgtaatcg ccccgcgact ttctttgtca gggcctcagg
     5221 tctcagcatg gtagacgccg gaataaacga cggcgccata ctggttgtgg atcggtcact
     5281 gactgcggag cacggctcaa tcgtcgttgc gctggttgac ggcgagttta ccgtgaagat
     5341 tttgcagacc ttcccgaaac tgatgttgat gccctccaac tccgccttca agccgatacc
     5401 tgttaacccg gacactcttg aaatctgggg cgttgtcacc tttgcgttga atcagtttcc
     5461 tcatgtacat gcacgttgat atcaatggag cctacgcggc atttgaatgc gccatggacc
     5521 ccaaacttgc caaatccccg ctcatcatcg cgagcaacaa cgatgccacc attatcgcca
     5581 tgaacaggct cgccaaaaat acagggctca aacgcggggt gcctatcttc aaatgccggg
     5641 atctgattga gaagcatcat attgcggtac ggagctccaa ctttacgttg tacgaagatt
     5701 attccaaccg gtttcatgaa acgctggagg ggtttgccga ggataccgat cgttacagcg
     5761 tggatgaaag cttcatgcta ctgaaaaaca tggacaaaat agtagatttt gaagagtacg
     5821 gatgcctgat cagacgaacg ctgctacaca atctttcgct cacctgcggg attggagtat
     5881 cgaccacgaa aacgctcgca aaattatgca cctacgccag taagcgatgg gcagcaactg
     5941 gtggtgtggt cgtccttaca gacccattcc gaattaaaaa actgctgagt ctcatcagta
     6001 cccgggaaat ctggggcatt ggccgcaaaa tcagtgagcg gctggctgcg tacgggatca
     6061 tcacggcctg cgacttttat gacagcgatg tgcggttttt acgaaaggca ttcggtgttg
     6121 aaatggaacg tacctggcgg gagctgcatg gcgagtcctg tttccggttg catgaatccc
     6181 ctcctgtcag acaacaaatt atcgtctcgc ggagctttgg ccagcgcctt aatacattca
     6241 gcaagatgca tgaggcagtg tcattcttta ccggccgtgc cgctgaacag cttcgaaacg
     6301 atggctcctg gactcgccaa atcaccgtgt ttatccagag cagcccgttt gccagagctg
     6361 aaacccggta cagcagctgc ggtattgagc cgcttactgc gacgcaggac acccgcgacc
     6421 tggtcagtgc ggcaggcgcc atactgaccc gaatctacaa acccggcata gattatgcga
     6481 aggcgggcgt gatgctttcg tcaatgaccg atggcactga gcagctatcc ctgttcgatg
     6541 aacgtcccgc caggcgaggc agtgaagcac tgatgaaggt gatggatcgg tttaacaaag
     6601 agcatcgagg ctctctgttt ctggcttgcg aagggattca tcaggatttc cgggggaagc
     6661 aggccatgct gtccccacgg tataccactc gctggaatga gttacttgtc gtcaaagcct
     6721 gacgttcatg ccggtgttac ccggcatggc cagtttcgga aggaaaagta actttagcct
     6781 ccacgggcct tctggattgc gtatttcagg gtggcctcgt caacagcgcc cgggatgaat
     6841 gacgtcgttg cagcgtcggg tttttgcatg ttcataataa tgaaccctgg cgtaccgtta
     6901 atcccgagag cttcccccag ctgcatgttt ccggagataa cgttctcata gcggcttttc
     6961 tcccgatcgc cgaatgagct gttgaacccg gatttgttca caaccatgtc taaatcattg
     7021 agtgtaaacg cacgctggtt gttgaagaat acatacgcac tggtcatcag attgttgtga
     7081 tacttacgat acgcttccgc accgaatgtc tgatacacat gcaggcctgt cgccgccccc
     7141 attgcggaaa cgggttttga gccagcgaag atcggaaatt ctttaaagaa gaactttaca
     7201 tcagtgcttt ggctcaacac agattcaaca accggcgcta cctgcataca gaaatgacac
     7261 tggtagtcga aaaactcaat aacagcgacg gccgcattat caggtccgat attgggtgtc
     7321 tctttggtct cgaagagtgc cggtgcgtag gggataatcc gctcaaggga agcgttagta
     7381 ttttcttttt ctagcgcctt acctgcttcg agaagatact ggggattttt taccagatag
     7441 gaagcagcca ctcgtcctat ttcgtcgtta cccggtacat cgggttttag atatttcgac
     7501 gtaatataca cagagccagc cgtcgcaatc accgcagaaa ttaaaacaga cgccacgggt
     7561 tttaaataat ttaatttagt catagccacc tttacgcatt tcaacttgtt agttatcagc
     7621 ctgcgttcga ttatactggc acggtaaatc ggaattattc attaaagaac cctttttgga
     7681 ggatttagaa tgttacggca atttttctta ctaacagcca caacgttctt atgtggatgc
     7741 ggttatcatt ttggaaatga cgttgatgcg tacgatttac tgccccgccc ggtaggcagc
     7801 aagaaatttg aaattgttgc tccggatgac agtattcagt cgcgaatgtt tgcagcccgg
     7861 tttgcgagcg gattaacggg taaggggttt aatatttcca gccatcagcc cgaatatatt
     7921 ctcagattca gttacagcaa gacccaggag aatctgcaat acagtgaact accggtgacg
     7981 ggaataaccg gatatgttat cgagaagaaa acaacgcgaa aagataagca cgggcaaact
     8041 gagacggact atgactataa gccggtcagt gggatcgtag gaacagagac ggtatcacaa
     8101 agacatttct ggcgtcgtct tgatgtggaa gtctaccctg cgggaaaaaa tgcacagcag
     8161 gtgctaaaag tcagtatgca gagcaatgcg ccgatcccat cggacagcgt ggcgtattcc
     8221 gcaatgattg acgctttgac cgggaatctg gatgccccgc tgcgctccgg aaactacgtc
     8281 gcctcggtcc cctggaactg atgaccggca ctgtaacgac caaccacaaa ttaacttttc
     8341 tcacctgcat gccacgtctt taccggcctc ctctgttgaa atcatagagt cggtacagat
     8401 caccgatggt cagctcatcc actttggtca cgaagagata ttcaaactga gctttctgaa
     8461 tatctgagac tgacgcatcg tccagcaccg tttggtaaaa tttggcattg atacatcttt
     8521 ccggatgcat gaactggtac cgcccctgga gcaccaccat cagaaagatc aatatcagca
     8581 gaacgagaag taacatctgg ttggaaagat ccagtcccag cgacacacca atggttgcca
     8641 tgaaacagag agtccatccc accagggcgt aaggagcctt ttggatcttt tgttggtaat
     8701 aacggaggat caccggatca tttaaatacc gcttatccgg caatcccgtt gcccgggtga
     8761 caatgctgag ccaaagcggc atcttctaac ccccagcgac gcactcaggc cagacgttca
     8821 tagaacgcaa caatgggctg gaagatgggt gtttgtacca gctcggggcg aatggctttc
     8881 agctgcctta ccggcgccaa ctcatccctc ggcttagcga tttcattgct agggcctttc
     8941 ttggtactgt cggtgttcac ctttaacctg tcattgctca gatacgactc aaaccgtttc
     9001 gacatcacgt cgctgcggga tgtcgcatca ttgagttttt ttaaaactgc gtctttatct
     9061 gtctgagcag cagataccag aatctcactg ctggccacag tgtcggcgtc gcaggccccg
     9121 aactggcatt tggcagcaaa gacgggttga gaaccaaccg ccaccagcat taacgttgtc
     9181 agtattgcag atttcataaa cacctcttgc agtcgtggaa agtcactttt gttcggcgcc
     9241 catgcgggtc accagatttt ccgggctcat ccaaccggtg aatagctgag gtttccccga
     9301 cagcgttacc atcgttggcg taccctgaag cggaatagtt gaagctacct ggaaatgcga
     9361 tttgatggta tcgaggcagg cctcgtcagg ccgtccttcc ggaatggttg atgtcgggct
     9421 cattgcggca tccagtgcat cattaggttt tgccgcacac catatttttg ccatattccc
     9481 cgcgacaacg gagttgagac ctgcacgtgg aaaggcgagg aatttgattg agatcccggc
     9541 gtcgagatag gattgaagat cttgatgcaa tttctggcaa aagccgcatg taatatccgt
     9601 aaacactgcc agacggtatt tttcattcgc cgacttatat tcgatggatt ttgttttagt
     9661 ggcgaactcc ctgacacctt tcagaatagc ctgctcagtt gtgttctcca catctttccc
     9721 gttcacatga aacaagctcc cggtgaaaat gtaatcgcca tctttgctga cgtagctcac
     9781 gccctctctt gaaatgacgg tataaatatc ttttaccggc gatggttcaa tatgctcgat
     9841 agacaagccc acttctctta atttcttcag tgcagcatcg ggaagcacgt cagtgttcgc
     9901 tgtctgggca ttcatactaa aggccataca ggatgccagt aaaaataatg atgccttgat
     9961 agtcttattc atttcagctc ctttaatgga gtgttaatac tatcatgcga aaaaatctgc
    10021 ctaattcgct taaggggaaa aattcccccc tatagcaaaa aaaattttga cggcgatatg
    10081 ggagaatatc cagaacgtaa ttccagcgga gattatctcg tgtttatacc accagtagac
    10141 gatgtgaaac ctattcccgt tcctgttgag atctacacgc agtgcattac cgatgcctcc
    10201 cgttttttcg ggattgatgc ggagttggtc tttacgcttt ttgataatga gggcggaaaa
    10261 gtggggacgt tcagccgtaa taaaaatggc acttatgata ttgggcctat gcagatcaat
    10321 tcgtccaacc tgccggaaat acgcgaccat tttccgtctg ttacgtggcg ggtgctggcc
    10381 tacgacgcct gtgccagttt ctgggtgggc acctggtggc tttaccggaa gattgttgac
    10441 cggaagggta acgtatttga agggattgcc gattacaaca gcaaaacccc taaggtccgg
    10501 gcgacgtata tcttcaactt catgatcaaa tacaatcgcc ggattcagcg ccgaaatgga
    10561 atggacgaat tatatcagtg gacgcaacct aagccccagt acaacgggca tattgtaaaa
    10621 aatcttcctg aataaaatac gaccctactc tccaattttt tattagttgt ttcgtagcca
    10681 taaacaatgc ggctcttaga gaatgaaaac gtcctgagaa aaaggtcaac gatgctccag
    10741 tcaatttata aattcctgtt caatttatgc agttcaccgc agcagcccaa aaataaagac
    10801 gtggatggga tattgtctca tgaagaaata accgctggat tatggggtcc tctgccatca
    10861 atccatgttg ttcttttcct ggattttgat ggtgtagtcc acaaatgtca gaatgaatcg
    10921 ttcgagagga tgcatttgat tgagaggtta cttgacgcga gtccttcaat gttcatcgtt
    10981 atatcaagct catggagaga gtgcggcagt atcacttacc taaagtcact tttccgctcg
    11041 ccatacagag acagggtaat tggagcaacc ccatccctgt atctcccctc aggaagtata
    11101 ggagtccgtg cagctgaatg tgaggacttc gtcaccagac acaaagtaaa agcctttatc
    11161 tgccttgatg acgatgcgac cctgtttcct ccagagtacc cgcacctgtt tcgtacagat
    11221 tattacaccg gggttaatga aaaggatcta acggacctga tagccagata taatgtgctg
    11281 atgacccgct tttattgaaa aagtaacttt tccacaccct ccatacaacc aaagcctctg
    11341 atgcgctata gcctaggcac taaagcatca gtcgattcag aaaagttact tttctctcaa
    11401 ttttcatttt gaacagaaga aatgcacatt ttcttagtgt gccgggtgac ctgtatttgg
    11461 tgaggaaatt tgaatcaggg tgatgcaagc aaaaaaagac cgtactcaaa agagagtacg
    11521 gcctttatca gctactactt acttttactt cttattttca ttcagccagc tttcagcttt
    11581 ctcaattctc gactgatatg gttcatttgt attattcagg tttgacttca taaagctcac
    11641 gaatgcatct gctttatccg gcccaaaacg tggatcatcg gtcagttgct gacgaatcgc
    11701 acgttctgag ttttgtgatg cagtctcata cgccttgatc ccatcccccc ccatatcctt
    11761 atacctgctg gtcaccgcgc taacaccatc tgacatttgc aggtgatttc caaccttgcc
    11821 caatagcgag ctcggcatat tttgtggatt cgaagcatcg tttgccaaag atgaaggggt
    11881 ggtcaggata cccatacctg agagcatgtc gtttacaggt ttagctccga taacgtcaag
    11941 tcctctggca acactctcct gccccgataa aagcgtacta ccaataacac cacgttctgt
    12001 attggactca attttattac caacaaggag atccgtcgtg gtttgggttc gttgcccaac
    12061 gagcccgccg ccagaaccac cttccccaag attcgccctc acaactgagt caagagaacc
    12121 tgaagccttg ttatgcatgt tgaaaccttc atggatattc tggagatcgt tcatccccca
    12181 gcgaacgtta tagtgactca gttcggagag gatattttga gtatccgctt tcagttgttc
    12241 tttctcagcg ccggttgcca ttgtactggc accataggta aacacagctt gtgcggccag
    12301 ttccgcacgt ttgtcatccg gctttttggt tggatcattt gcaatcctgt caaaataatt
    12361 cgggaggttg cctgatatac cgcgtatctg gttatctccg tacatattat tcagtttgtc
    12421 gctggtagta cccatgcccg aagtcgcatg cttaccgccc gaaccagcct gtaactggtc
    12481 gaaagtttgg ttggcgtgct gaacgtccac cccagccccc tgaactcttc ccacagtttc
    12541 attcgtcaca acaggggtat tcttatccaa tgacgagagc acattctggt tctcttcgct
    12601 ataggattta tttacattac cttgatgaat gccctgaata tcttctttgc tgaccttgtc
    12661 cacattgtac ggcgtaacac cagactcagt ttgtccgcca acatgctggt tagcattttg
    12721 ttggttttgt accagcccgg cttttgcctg agcatccata ctttgcccca cgtcagcaac
    12781 acgatcagcg ctcattacgc tggaggtatt gacatgttct ggggtgcgat cctgcgcctg
    12841 tgcaatcgtg ttaatacccg aggcatcacc agtgattcgg tcaagctgat tggtaatcgg
    12901 cagcatctgt tgagcgttac cgccgaagtc agcaaccagc cctttaagca ggctggaagt
    12961 ttcacgcaga tcctgtttat tgctctcagc cgtttcacct cgggcagtat caatcgctat
    13021 tttttgctca ctaaagtcac gtgtcgtggc caccagcgca tctgtacgtt ggagttgtga
    13081 acccaactga gtgctggcct taaatgtgtc attataggaa ttgaacttct ccataaaggc
    13141 attttcatca agaccatttt tccgtgcaaa attacgcaca tcttcatcgc taaagttttt
    13201 attacggata gaatcggaga atctgtccag gttgatagcc tgtcttgagt cgagtgtcat
    13261 gccagtattg gcgctggcgt tcgttgaaag tgacgtcgac aagttttcgg ccattgtctg
    13321 tgtggactgg ttcatttttg ttgcagcttc tttgaatgca ttggtctgtg caaactgatc
    13381 actgtttatc tgagcagccg ctttagtcat ttgatcagtc aggcctgagt tttcagtcaa
    13441 ttgagtcgta atagctttac tcaaatcagt taagagactt tcattcccag aattactttt
    13501 cgtgatccct gatccaactt gcccccccgc ttttattcct aggctatcca ttgctttctg
    13561 agcgagacct cctcctcctc cttttccaag tttttcttca ccaccaagtc ccaaactgcc
    13621 attgagcaga agatttgaca ccacgccagc taattgctgc gaattaattc catgtttcgc
    13681 ccctacgcta ttcgcgatgt ttgcaccggc ctggtcagta aatgatttca agctttgcag
    13741 gttggaggta aattgctggc ctgattgccc cgttcggcca acttcactca tctggttgaa
    13801 ggcctgctgg aatgtgttac ttgctgcgct catggctgaa gaggtaaatg ctgctccttg
    13861 cccctggcta gacgcgatac ttgaaccggc attccacgta ccgagtcgga aattactgga
    13921 gtccatggcc ccgccaccac tgaatccttg gccggtgctt gtcagcgcgg tacgattcac
    13981 atccccaaac gatgattttc cgctgtttgg cgcatcccac actttcggcg tgaggtggct
    14041 ggagtccact ggcgctggtg gcgtgactcc tttcatcgcg ttcatcattg gatgaatgga
    14101 ttgcgtcatc aggaacagcg tcagtactgg gataagagca tagagtgctg atgctactga
    14161 taaataagag ctgtatgttt cggcaagccc tggcaaaccc atccatgtca ccgcgttact
    14221 ctgactattc aatgtgctcc atgtatcaag atctgctgtc gccactttct tcacgtaggc
    14281 attcaccata accgcagtaa taggccacat atttacgaaa agaataagct ggagatattt
    14341 tgcagcagcg gccacaccat ttcccccgag agccaaaagc atgagcaatg caaatggggc
    14401 aaccatgtac gcaaacatct caagaaacgc gatggctgca ccggacaatt gcagccagag
    14461 ttgcccctgg gatgccatgg tattcgtacg ctttaatgat gcctcaaaca actgcatatc
    14521 cgaggccagc ccgagtgggg ttttgtactt gctggcacca ttacggagtt cattcatgat
    14581 gaacaaacta agcgtcgcgt cataagcacc aatagctttg ccgtacatgc cattcgccga
    14641 cgccattaca tccgtgaagc tcgatcctga tgctgcacct cccgcctcat ctggggcaag
    14701 gataccgttt gtttgcccaa tcaatgcaat cgtctgccga gcctctgggg tgctcgttac
    14761 ctgtttaacg gtcgaccaga cctcttcaca agtagcagac tttgaactat tcacaccgac
    14821 aatgttggca gaggcgttct tcagcgaacc tttcatcgcc tgattaatgc gttccatgct
    14881 atccacgata tttgcagaga aaatatcgtt aaaaatggtt tctttcccag caatttttgc
    14941 gcttgagttt tgggttccct tgagacagtt ataggccagt gattgtactg tcgcgcacac
    15001 attcatcgca cctaacccgc tggctggttc gggaaacgca gaacaatagc cctggctgtc
    15061 accaccccat tgtaagaatt taacgaaacg aatcatcgga ccaagagtaa tgtcatcatc
    15121 aagcgtcgtt gcggcaaagt cgatcggcgc cagtggatca aatgccgttt tgtagtcttt
    15181 gagtaagccc tggctaaggt tcgtggtgac agttcccatc gccgcaataa aaataggaat
    15241 gccatcgaca tttctcactt caccagactt aactgattca atcgtcacat ccacacgaac
    15301 aattgccatc gaaaatagaa tcagcccaag aatccatgaa aatatcggca cttctgcttt
    15361 tgtcgggttc aacagccaaa gccacgtttt ataaaacagg ctggctccag ccagtaccgc
    15421 tgccgtcgtg agaaatgctt ttacaccggt gtattgtgag aaaatcaggg caatcccgtt
    15481 cagggcggac cacacaaatt caatatcgcc aactgtatag atgttgtaat ccatatcaga
    15541 cctcagttac ctgtgccacg aagactttcc atgaacgctt ttctctttaa tcggcgatca
    15601 tcccatgcag aaatgacgtc agagctgttg cccacctggc tacgggttag ctcataggca
    15661 gcttttacct gattggtaag gttttcagat aacctaatca ggtcctgccg ccgcccggaa
    15721 agagattcgc tgggaatatt catagcgctg atctttgctt caacttgccg aagcatgtta
    15781 ataacaaggc tggtggccgc tattgcactg atatcctgga tataggcgta gccttcaggg
    15841 acatcaaacg tctgaagaga atcaagaatt gttggaatgc cgatataaga aataatgcgt
    15901 aattcatcat cagagatcct gacatcgctg attgcctttg tctgaacctg aataaggagg
    15961 tccgagattg tatccttcag acctttgaaa ccaccatccg tcacctcaga cataaccaga
    16021 cattgcgact ttctcgctga agctggatcg ggtgcaggcg aacacttgag catttttatg
    16081 gaaccaccag ccggtggacc catgatgaaa tccgataccg tcatcgtcga cgggcgaact
    16141 tccatcccag ctttttcacc tttagcatcc cagttgataa ttaccgtacc aaccagcgac
    16201 atcagctgct ctgcgagttt gtagccagat aactctgtca caccaccgat attcatagaa
    16261 cctttatcga tgtccataaa tgacatataa aaaaggtttc cactgaattt ttcagcgaac
    16321 ccttcaggat cttttgcttt tacctgggcg ctcacggttt ctggcgcctt ggtcattgac
    16381 gaaccaaaat cagacaacaa tccattaaga gatccaagca ctgaagcagg accggaactg
    16441 actgcattcg caaactggct gggaggtcca ttagtttcgg aaagaaacga ataagtagcg
    16501 ttgcaggaat ctttggcaaa tttattcaac gcctgcaatt tattctggat gtcgttaatc
    16561 gtcgcagcac agtcagcaca gattgccgat accgcgacat tgaatgcata aattgcagcc
    16621 ccctgtgcaa taccgcgagc aacctgaacc agttgatctc cgttaatcat actgaaggaa
    16681 ccaaggaaaa catcgattcc gttgcagcct acggaagctt tgggaaagga cattgacact
    16741 aaattggtat tcacgtttgt cgtgcggtag gaaaaggagc cgccaaccac tccggtacgg
    16801 gtggccgttt caaaactggc tgggctcgta gtcgtcatca tgccattgaa aatattacgc
    16861 atggcgttat cggcaacggc atttggcgca atcatcgccg tgcaaacggc taacgataaa
    16921 agtaactttt ttgcacaaac atggagatca gcgcgcatat gcacctccta aactgccacc
    16981 gacattcagg tagttcactg ggtccgccgt tggcatatcg tacccttcca gtttcttatc
    17041 cattattcgt tgcaataaga acgggtcaga gtccatttct gatttattca cagtaattac
    17101 gccatcctcg ccaatggtat attggcgctt aatatctaaa gtggactgga atgaggcatc
    17161 atcaatcagc ttcatgcctt ttgcagccag aataatagta ttcttgagct cctcagcagt
    17221 gatcatgcct tcgctaattc gttgcgctga tgacccatct tttgagacaa ggaaaatagt
    17281 tggaacttca cggattttaa attggtcaat aatctgcgca ttaggtacag agaaatcctg
    17341 gaacaaccca ttctgtagtg gtctgccgtc catgctgaca ggaagaatgt cgacagaata
    17401 gtaattctcc ataaactgga ggatttgact ttcttcgtgg cagaactgac aagtactctg
    17461 gaaaaagaaa aacagcccgg atttagtgaa aatatctttc ataaccgact gctgattttt
    17521 ctcgaccacc gtacgatgtg catcgagcgc taccttttca accggttgcc tgcgtttttc
    17581 agacatcatt ggatttttaa ggaaataatc tttcgattta tccgaaaacc gcgtaccgat
    17641 atccagcatt aaacgttgtg ctgtataaaa tctcgataaa ttttcagggg tcggattatc
    17701 catagcctta gtcaaaagct gaggcatatt gtcctttagc cactttgaat taagctcaat
    17761 cttttcttct tgtgttggct gggtcgaaga tgcaacagga ggcttggtct ctgtaatttc
    17821 ttcctcagtt ttccttgtcg ggtcgtcata ccagaaaaag cctttcttga aggcctgtcc
    17881 tgatacaaag ggagtgtctt tgataagggc ctgctcttca tttgcatgga ccccagtgat
    17941 aaacaaagac agcagcacta acatctttga aggtattaac gttaatcgag tcatttaccg
    18001 atctcctcat ggaatagagg tatgatggca tagaaaaaaa cttcatgagc tcggtaacgg
    18061 ggtagatttc aaacgttaaa aagtcataaa tgtgactact agaaagaccc tgaaagggct
    18121 gttgaacttc tgtgtgcagg gaaggtatgg gagctaaaaa ggtgggaaaa tagttatcag
    18181 catagttaat tgaatctgaa ttcaattcca gacgatactt ttaaaaattt ctagatgagc
    18241 gccgcattcg attgattttc gaatcgggaa ttttcgataa attaaaaaaa ctgaggatat
    18301 attatttggc aagaaaacgg tttaatgtgt ttatatcata tgcagtattg acctaaaaaa
    18361 tgccatttca tgttagtcat acttatgcag agaaaagtta ggcagaattg actgggtaat
    18421 gagcaactac ggttgaaaat aatattagca taatcgtttc agttacgtta ggccggaagc
    18481 tgtctgaaga aaacagcaac atgtagtcta ttttgcagtg aagtaagtaa agaaattttg
    18541 tagttaacga agaaacatcc ctgggaattt tgacctctac ttaaatcact cacataaaat
    18601 aacagctaat ctaactcttc ctgggaatac cgtgctattg ccgatgacgt ggacgacaat
    18661 ctgcatcgag gacaatgagt atcattggta tacgggacaa tataagggcg cttacatttc
    18721 gcacaagttt caagctcctc gatttctttc attgacgtta gaatctcaat gattttattt
    18781 acctcaggag cgcagacgaa atatccaagg ttatgcagca cactcatgat gcggttgaag
    18841 tcaatatttc gaaaactgga ttccgggtga ataggatcaa aaccgaaata gtttacagcg
    18901 acatcaataa ccgcagaaaa agccctgcgg ttatccgtgc ggcgactcca cccacgaatc
    18961 ccttttttaa cgttttgt
//
LOCUS       DAFNBJ010000094        18936 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000094 DAFNBJ010000000
VERSION     DAFNBJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.094"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..354
                     /locus_tag="L2X49_003098"
     CDS             <1..354
                     /locus_tag="L2X49_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4798139.1"
                     /translation="ITDNSTYEQIIAEEFTQLISKSAGVDDIQMEIDERFMNRKISFR
                     GSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLYISYNKSSLN
                     NLVFSRFIDRLNESF"
     gene            501..1721
                     /locus_tag="L2X49_003099"
     CDS             501..1721
                     /locus_tag="L2X49_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBN4798140.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1694..2323
                     /locus_tag="L2X49_003100"
     CDS             1694..2323
                     /locus_tag="L2X49_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBN4798141.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2324..3484)
                     /gene="ybdL"
                     /locus_tag="L2X49_003101"
     CDS             complement(2324..3484)
                     /gene="ybdL"
                     /locus_tag="L2X49_003101"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBN4798142.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3593..4681
                     /gene="hcxA"
                     /locus_tag="L2X49_003102"
     CDS             3593..4681
                     /gene="hcxA"
                     /locus_tag="L2X49_003102"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBN4798143.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHDLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4691..4888)
                     /locus_tag="L2X49_003103"
     CDS             complement(4691..4888)
                     /locus_tag="L2X49_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBN4798144.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5070..7175)
                     /gene="cstA"
                     /locus_tag="L2X49_003104"
     CDS             complement(5070..7175)
                     /gene="cstA"
                     /locus_tag="L2X49_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBN4798145.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7356..7769)
                     /gene="entH"
                     /locus_tag="L2X49_003105"
     CDS             complement(7356..7769)
                     /gene="entH"
                     /locus_tag="L2X49_003105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBN4798146.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7772..8518)
                     /gene="entA"
                     /locus_tag="L2X49_003106"
     CDS             complement(7772..8518)
                     /gene="entA"
                     /locus_tag="L2X49_003106"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HBN4798147.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8518..9375)
                     /gene="entB"
                     /locus_tag="L2X49_003107"
     CDS             complement(8518..9375)
                     /gene="entB"
                     /locus_tag="L2X49_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBN4798148.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9389..10999)
                     /gene="entE"
                     /locus_tag="L2X49_003108"
     CDS             complement(9389..10999)
                     /gene="entE"
                     /locus_tag="L2X49_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HBN4798149.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11009..12184)
                     /gene="entC"
                     /locus_tag="L2X49_003109"
     CDS             complement(11009..12184)
                     /gene="entC"
                     /locus_tag="L2X49_003109"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HBN4798150.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12559..13515
                     /gene="fepB"
                     /locus_tag="L2X49_003110"
     CDS             12559..13515
                     /gene="fepB"
                     /locus_tag="L2X49_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBN4798151.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13519..14769)
                     /gene="entS"
                     /locus_tag="L2X49_003111"
     CDS             complement(13519..14769)
                     /gene="entS"
                     /locus_tag="L2X49_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBN4798152.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAIMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14880..15884
                     /gene="fepD"
                     /locus_tag="L2X49_003112"
     CDS             14880..15884
                     /gene="fepD"
                     /locus_tag="L2X49_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBN4798153.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15881..16873
                     /gene="fepG"
                     /locus_tag="L2X49_003113"
     CDS             15881..16873
                     /gene="fepG"
                     /locus_tag="L2X49_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBN4798154.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16870..17685
                     /gene="fepC"
                     /locus_tag="L2X49_003114"
     CDS             16870..17685
                     /gene="fepC"
                     /locus_tag="L2X49_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBN4798155.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17682..18815)
                     /gene="fepE"
                     /locus_tag="L2X49_003115"
     CDS             complement(17682..18815)
                     /gene="fepE"
                     /locus_tag="L2X49_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBN4798156.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4495 a         5323 c         4843 g         4275 t
ORIGIN      
        1 attactgaca atagcactta tgaacaaatt atagcagaag agttcaccca attaatatcc
       61 aaatcagcag gagttgatga tattcaaatg gaaatagacg agagatttat gaatcgcaaa
      121 atctcatttc gtggttcctc tttgctgaca atcattaata gcattgcagt tacagattta
      181 ctgggaatag tcccctatga actttataat tcctaccgtg actttcttaa tttaaaagag
      241 ataaaactag aacatccgtt accttcaata aagctttaca tttcctataa taaatcttct
      301 ttaaataatt tggtgttctc ccgatttatt gaccgtctaa atgagagttt ttgatatata
      361 actacagaca gctactataa ctttcatcta tttattcaca gcgcgaatga aaaataaaac
      421 actctgcttt acagtgttta ctttataatt aacagtctta tctcactgtt caccctgtaa
      481 cgttcagaga gcgtcttcag atgtctattt ataaaattcc tcttccgctc aatattctgg
      541 aagctgcacg agagcgtatt acctggacac tcaacaccct accccgcgtt tgtgtctcat
      601 tttctggcgg caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacaaa
      661 tgggcaaaaa aatctgcgtt ttgtttatcg actgggaggc gcaattctct tgcaccatta
      721 actatgttca gtccctgcgc gagttgtaca ccgatgtcat cgaagagttt tactgggttg
      781 cgctcccgct tacgacgcaa aattcccttt cacaatacca acccgaatgg cagtgctggg
      841 aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc gataaccgat cctgactttt
      901 tctgctttta ccagccaggc atgaccttcg aacaatttgt acgtgagttt gccgaatggt
      961 tttcacaaaa acgtccggcg gcgatgatga tcggcatccg tgcggatgag tcctacaacc
     1021 gttttgtcgc catcgccagt ttaaataaac aacgttttgc cgacgataaa ccctggacta
     1081 cggccgcacc aggcggtcat agctggtaca tttaccccat ttacgactgg aaagtggctg
     1141 atatctggac ctggtatgca aatcatcaaa gtctctgcaa cccactgtat aacctaatgt
     1201 atcaggcagg cgttcctctg cgtcatatgc gaatttgcga accttttggc ccggagcaac
     1261 gacaaggatt atggctctat cacgttatcg aaccggatcg ctgggctgct atgtgcgcac
     1321 gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca tgacaatcat ttctatgggc
     1381 atcggaaaat cctcaagcca gaacatttag actggcaaga atatgcatta ttgctgctca
     1441 atagcatgcc ggaaaaaaca gctgagcatt accgcaataa aatcgccatt tatttgcact
     1501 ggtatcagaa aaaaggcatc gaggttccac aaacccagca aggggacatt ggcgcgaaag
     1561 atatcccctc ctggcggcgg atatgcaaag ttttactcaa taacgattac tggtgtcggg
     1621 cattatcatt tagtcctacg aaagcgaaga actatcagcg ttataacgaa cggataaaag
     1681 gaaaacgtca ggaatggggg atactatgca acaacgatta acgcaggatt taacgcaatt
     1741 tctcgccagc ttgccggaag atgatcgcat caacgcgatt aatgaaatcc gcatggcgat
     1801 ccatcaggtt agcccctttc gtgaagagcc agtggattgc gttctgtggg ttaaaaacag
     1861 ccaacttatg cctaatgatt acaacccaaa caacgtggca ccacccgaaa aaaaactgct
     1921 gaaaaaatcc attgaaatag atggttttac ccaacccatt gtggtcacgc atacggataa
     1981 aaatgccctg gaaatcgtgg atggttttca ccgccatgaa attggtaaag gctcatcatc
     2041 attaaaatta cgcttaaaag gttatttgcc ggtgacctgt ctggaaggta cgcgtaatca
     2101 acgcatcgcc gcgactattc gtcataatcg cgctcgtgga cgccatcaaa tcaccgccat
     2161 gtcggaaatc gttcgtgaac ttagccagtt gggatgggac gataataaaa ttggcaaaga
     2221 gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa attaacggtc tgcaagagct
     2281 gtttgccgac cgtcaatatt cccgcgcctg gactgtcaaa taactacagc tggcgcagtc
     2341 gttcagctgc tgccagcaac gtcgattcct tcttggcaaa acagagacga atcagtttat
     2401 gagggaaggg atcggcgcaa aacaccgata gcggaatcgc cgctacgccg tgctcctgcg
     2461 tcagccactg gcaaaactca acatcatcca gggtagatac ggcgctgtaa tccaccagca
     2521 aaaagtatgt accttcacac ggtaaaatct ccagccggct ttcatttaaa gcattcacca
     2581 gaatatcgcg cttctggcga taaaagtccg gtaacgcaag ataatgctca ggttctgcac
     2641 gtagcatatc agcaagcgcc agctgtgccg gggtattcac cgaaaaggtc agatactgat
     2701 gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac acaataaccc actttccagc
     2761 cggtcatatg ataggtcttg ccaaatgaag aaaccgccac tgcccgctca cgcagctgcg
     2821 gatgcgccag cacactggca tggccctgtt gtgaaaagtt gatgtgctcg tagacttcat
     2881 cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca caaagcggcg aaatcagcct
     2941 gctgccagac agttgcactg gggttatgcg gagtgttgag gatcaccagt ctggtgcgct
     3001 cgcttaacaa tgcggcaaat tcctgccagt caacgcgaaa atgcggtggt tgcagtgcca
     3061 tacgcttcac tattccccca gaaagcgcga tggcggggga gtaactgtca tagctgggat
     3121 caaaacaaat cacttcatcg ccattgcgca ccagtgcggt aatcgccgcg tataacgctt
     3181 ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc tggttgatag ccatacaaac
     3241 gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac gccggtcatg ggcgcgtatt
     3301 ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg taaatagcgc ggaccatcaa
     3361 aatcaggaaa gccttgcgac aggttaatcg cctggtgttg ctgcgccagc gcgctcatct
     3421 gggtgaaaat agtggtgcca agttgtggaa gtttgctttg tggaatcaga gggttatttg
     3481 tcatttttat tgtacctgac gaagttgcgt gttgaaggca ctataacacg atgctagtat
     3541 ttggcaatca agacgtttag atgtctaaat aaaacaataa ggacaacaca acatgcctca
     3601 caatcctatc cgcgtggtcg tcggcccggc taactacttt tcacatccag gaagtttcaa
     3661 tcacctgcac gattttttca ctgatgaaca actttctcgc gcggtgtgga tctacggcaa
     3721 acgcgccatt gctgcggcgc aaaccaaact tccgccagcg tttggacttc caggggcaaa
     3781 gcatattttg tttcgtggtc attgcagcga aagcgatgta cagcaactgg cggctgagtc
     3841 cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc gcactgctcg acaccgcgaa
     3901 agccctcgcc cgccgtctcg gtctgccatt tgttgccgct ccgacgatcg ccgccacctg
     3961 cgcagcctgg acaccgctct ccgtctggta taacgatgcc ggacaggcgc tgcattatga
     4021 gattttcgac gacgccaatt ttatggtgct ggtggaaccg gagattatcc tcaatgcacc
     4081 gcaacaatat ctgctggcgg ggatcggtga cacgctggcg aaatggtatg aagcggtggt
     4141 gctggctccg caaccagaaa cgttgccgct aaccgtgcga ctggggatca ataatgcgca
     4201 ggccattcgc gacgtattgt taaacagtag cgaacaggcg ctgagcgatc agcaaaatca
     4261 acagttaacg caatcatttt gcgatgtggt ggatgctatt attgctggtg gtgggatggt
     4321 cggtggtctg ggcgatcgtt ttacgcgtgt ggcggcggct catgccgtgc ataacggtct
     4381 gactgtcctg ccgcaaaccg agaaatttct ccacggcacc aaagtcgctt atggcattct
     4441 ggtacaaagc gccttgctgg gtcaggatga tgtgctggcg caattaaccg gggcgtatca
     4501 gcgttttcat ctgccgacca cgctggcgga gctggaagtg gatatcaata atcatgcgga
     4561 gatcgacaaa gtgattaccc acaccctgcg tccggtggaa tccattcatg acctgccagt
     4621 cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa gtggaatcgt ttaaagcctg
     4681 acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc gtagcgcgcg tcctgccgct
     4741 cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg atggttaacc cgcatatgtt
     4801 cgacatagtt gtcgtaatca ggcataccaa tcatcagctt cgccgcctga cctaaatatt
     4861 ttccggcttt tgccagtgaa tcaaacatct tggcctccgc taaccatgtt gctgaaagag
     4921 tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg ctatcaacgc gctcggtcag
     4981 tcccctcgcc ccatcgggga gagggttagg gtgaggagga accctcggca ctatgccaac
     5041 attcgccaac atcccccctc actctgactt tagtgtgcgc cttttgcctg cgccacgatc
     5101 tcctcgacat tttccggcat tggctcatac ggcgtttctt tcgccgttgg cttcggatct
     5161 ttcaatgccg ccagtgccgt cttaatcgag aacagcgcca gaaccaccac gaccaccata
     5221 aagaagatgg ttaacccggc atccagacgg ttgttgaaca ccagttgcgc cagctgtgac
     5281 tcagtatact gcgacggaat attgccgctg tcgatcattg cctggaactt attagcaatg
     5341 gccaggaagc cgactttcgc atccgggcta aacgctttct gccagcctgc ggtcagggta
     5401 caaatcagca gccaggccgt tggtaccagc gccacccagg cgtaacgttg acgtttcatc
     5461 ttgaacaaca ccacggcaca gagcatcagc gccatccctg ccagcatctg gttggcaata
     5521 ccaaacagcg gccacagagt gttaataccg cccagcggat cgaccacgcc ctgatggagg
     5581 aagtagcccc acgccagtac gcacagcgcc gttgccagca ggttagcagg cagtgaatcg
     5641 gtccgcttca ggccaggaga caccacgccc agcagatcct gcaacataaa gcgcgcagca
     5701 cgcgtacccg catctaccgc cgtcagaata aacagagctt caaacagaat ggcgaagtga
     5761 taccagaacg ccacatccat cataccaccc agcgcaccgt gcaggatgta agccatcccc
     5821 accgccaacg tcggcgcacc gcccgcacgg gaaatgatcg actgttcacc cacttcgctg
     5881 gcaatctggt tgagcgtatc tggtgtaatg ctaaagcccc agctactcac cacctgcgcg
     5941 gcagaagcga ccacatccgc cgtccctgcc ggagccagca ccgccattgg gctgttcatg
     6001 gcaaagtaaa cgcccggatc gatgatacag gcggaaacca gcgccataat cgccacgaag
     6061 gattccatta acattccacc gtagccgata aaacaagcct gcccttcgtt cgccagcatc
     6121 ttcggcgtgg tgccggaaga gatcagcgca tggaagccag acaccgcacc acaggcgatg
     6181 gtgataaaca ggaacgggaa caggttaccg gtccataccg ggccagtgcc atcgacaaat
     6241 ttggtcagcg caggcatggt cagcgtcggg cgcataatca aaatgcctac cgccagaccg
     6301 acgatagtcc cgattttcag gaaggtcgag agatagtcac gcggggccag cagcaaccac
     6361 accggcagca ccgccgccac aaaaccgtag cccaccagca tccaggtcag ctgcacgccg
     6421 gtaaagtcaa agtacggtgc ccaggtcgga ctttctgcca cccagccgcc agagataatg
     6481 gcgaaaatca ggaacaccag gccaatcact gacacttcgc caatgcggcc cggacgcaga
     6541 tagcgcaggt agattcccat aaaaatcgcc agcggaatgg tgaacgcaac ggtatacgtt
     6601 ccccacgggc tatgagtcag ggctttcacc acgatcatcg ccagtactgc aaggataatg
     6661 accatgatca taaagcaggc taccagcgca atcaccccgg cagttggccc catctcttct
     6721 ttgaccagct cacccagcga tcgaccgtca cggcgcgtag aaacaaacag caccatgaaa
     6781 tcctgcaccg caccagcgag aaccaccccg gcgagcagcc agatcatccc tggcaggtaa
     6841 cccatttgcg ccgccagtac cggcccaacc aacggacctg ctccggcaat ggccgcaaaa
     6901 tggtgaccga acagcacttt cttgtccgtc ggcacatagt ccagcccgtc gttatggcgc
     6961 accgctggcg tcatacgcgt cgggtcaacc gccagcacat ttttggcgat atacagccca
     7021 taaaaacggt aagcgatcag atagatacag accgacgcca ccacaatcca cagcgcgttg
     7081 atctgttccc cacgatttaa agcaatgtat cccagagcaa atgctcccat tacagagagc
     7141 actgtccaga cgaggtattt ccctgatttg ttcatagttg ttatccgtgt gcgtgtccat
     7201 agagatgtta cattttgttt ctataacatc tttgtacgat ttaacaacac ggtaaccatg
     7261 taaatcggag ttgaaaccaa tatttaactt aacaatgtta actcgatcac tccgttaacc
     7321 gagttttcct gcaatctcat ttaattctgt cgggctcatc ccaaaactgc cgtacccagc
     7381 cgacaagtgc agcaacgccg cccctgttca tcgaaaacga cgatttccca gctctgattt
     7441 tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct tgccttcaga caccgggcga
     7501 tgatgggtgg cgttaagttc cgtacccacc acacactgcc cgtcacgggt catcataaat
     7561 ccggccatcg atcccagcgt ttcagccagc gccgccgacg cgccgccatg cagcaggcca
     7621 aacggctgat gagtacgggt atcaaccggc atttcggctt ccagcacatc atctcccaga
     7681 cgggtataca caattcccag atgcgccacc attgtgttat cgctggtggc gttcagttcg
     7741 tcgagcgtta aatggcgttt ccagatcatg cttatgcccc cagcgttgag ccgccatcga
     7801 ccacaatatc ctgtagggtg atatggctgg cgaggtcaga ggcgaggaac aaaatcgtgt
     7861 tggcgatctc ttgtggacgg gcgattttcc ccagcggaat gccgagttta aactgctcgc
     7921 caaagccgcg aatacgctgt tcttcggcgt catcgctcac ccacagcgtg cgttgcatat
     7981 cggtgtcggt ggagccaggc gaaaccacat tacagcgcac gccgctaccc gccagttcca
     8041 gcccgacgct caacgccagg cttttcagcg ccgctttcga tgcgccataa gcactcatac
     8101 caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac aatcgccccg ccccgctgac
     8161 ggcgaaactg gttcatggtt tgctggaaca ggttaaacgc accgccgacg ttaaccgcaa
     8221 aagtctgctg ccagtcctct ttactgagct gatcggtcgc gcccatgcgc aaaattcccg
     8281 ccgcattgat cagcacgtcc agtcgctccg tttcagctaa cagtcgctga catacctgcg
     8341 ccacctgccc ggcgtcggca acatccatta cttcggtcgc aaagggatat tgctcctgag
     8401 tgaacgcttg atcaaaacct gtaactttcg ctcccgcctc aacaaacgcc agcgccgtgg
     8461 cgtagccgat acctttacct gcgccggtta cccagacatt tttaccgctg aaatccatta
     8521 tttcacctcg cgggagagta gcttccacca ggcgtcgatg gtcgggtttt tcgccagcat
     8581 gacaaagtcg atatcaccat gcactttgcg ccagcgcgcc gccagcgcca tcatgcgcac
     8641 cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac ggttcatcgg actcgtccag
     8701 caacggcagg ataacctcac gcagcgccgc tttgctggcg gggacaggtg ctggcagtaa
     8761 ttcttcagtc atcaccaccc ggccagaccg cccggccaca tatttcagcg acatcaaatg
     8821 ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata aacggtttaa tatcgcgcat
     8881 aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca tataccccgg taataatcag
     8941 ctggttacgt ccactctctt tgagcatttg ctccagcgga gaacgatgaa acgcgctgta
     9001 gcgccacttc accagcaccg tgtcgtcggc atctggcgtc aggcgatcca ccaccttttg
     9061 ctgttccggc gaacgggtca ggcccggccc ccacatatca ttcaacagcg cccgatcttc
     9121 atcgctctgc tctttcggct gggcggtgta ataaaccggg atattgtgct gtttgcagta
     9181 gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc atcgggcagt tctcgcccca
     9241 gaagctgaca aaatagtcct gcatatcatg gattaacaac gcggcacgtt gcggttcaaa
     9301 ggcccagtca actttattct gcggaatatc gtgagactcc ggcagtgcgt aagcctgtaa
     9361 ttttggaata gccatagtgt tctctccttc aggctgatgc gcgtgacgcc agccactgac
     9421 gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg aagtgaatcc acacactcca
     9481 cgcgatccgg taatttaaat tcggcaatac cctgttcacg caggaaacga cgcacctgca
     9541 ccgcgcgcag cggctctttt accaccagat aagcgcagct tttttcgccc atcagctcat
     9601 cttccatgct caccagtgcg gcatagatca ctgccggatg gcgcagcagc aggttttcga
     9661 tctcttcggc agcgatcttc tcgccgccac ggttgatctg atctttctcg cgcccctgca
     9721 cggtgatgta accctctgga tcaatagaga tcagatcgcc ggaacagtaa aaaccgttgg
     9781 catcaaaggc gctggcattg tgctgcggac ttttgtaata accgcggaag gtgtacggcc
     9841 cgcgcgtcat caggcgtccg acttcccctt gcggcagtgg atttccttcg gcatcggcaa
     9901 cccacacttc atcgtccgga cacattgggt aaccctgggt atggataatt ttctccgcgc
     9961 tatcatcaag acgggtgtag ttcaccagcc cttccgccat gccaaacacc tgctgcaact
    10021 gacagccaat ctcagcggga atacgcgccg caagggtggc agaaagacgt gcgccgccga
    10081 cctgtaacag tttcagcgag gcaagctgcg cccggctttc gccttcggtc agcgcctgca
    10141 accacaggct gactgcgggt ggcaccagcg cggtaacgtt aacctgatgt ttttcaatca
    10201 atgggaaaca gagcgtggcg ctgggatcgg ccgccagaac aaccgttcct ccggcaagaa
    10261 agacgcccag cgatcccggc gaactcatgg cgtagttatg agccgccggg atcgcgcaca
    10321 ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct acgacgcacg ctgtagtagt
    10381 agtcgttatg agtgcgcggg atcagtttcg gtgtgccggt ggtgccgccg gaaagctgga
    10441 aataggccac ttcatcagca ggtgatggcg tggcagtaaa atcctcagcc ggatggttaa
    10501 tcgcatcctg caagttatgc tcaccgctgt cgttgtgcag ttgcaccacg cgaatggagg
    10561 aatgttctgt gacgaaagta ttgaggaaat catccccgct aaacagcgca tgttggcgat
    10621 cggcaatcag caatgcgggt tcaatctggc tggcataggc gttcagttca ctacgctgat
    10681 ggctgaacag cgccagcacc ggcgcaacgc ccagtttcag cagcgcgaaa aaggtaatat
    10741 acaattcagc gacgttaccc agttgtacca gcgcggtttc accaggttta atgccctgac
    10801 ggcgtaaact acacgcgagg ttatccgccg cctgattcag ctcccggtaa ctcaactgtc
    10861 gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg gcgagtcaga atgtcggtca
    10921 gcggcaaatc ctgccagtag cctttttccc gatagcgacg ggcaaactct tccggccagc
    10981 gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc aaaaacgttc aacatggtag
    11041 aaagtttgac gcctgtttcg cgccactcac ccaacggtga cgacgcaggc acaatccccg
    11101 ctccggcaaa cagacgcacc tgattttccc gcagcttcgc gcagcggatg gtcaccaccc
    11161 attcgccgtt accttcgctg tcacaccaac ccacaatgcc gccaaacagt tcgcggtcga
    11221 atggctccag ttcagcaata acctgggtcg cggcctgatg cgggaaaccg ctcagcgcgg
    11281 gggttggatg cagcagacag gccagagtca gtgcgttttc ttgcgaattc gctttacctt
    11341 caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat caattgtgga gaggagggaa
    11401 cgtgtaactc actactgcgt tcgcgcagta cctctttcat cgcctgagtc accagttcat
    11461 gttcatggcg atctttttct gacgccagca gacgattacc cgcttcgcga tcgagcactt
    11521 catccggctg acgacgcgcg gaaccggcta acggaatgga gctaaaacgc tcgccgtctt
    11581 tacgtagcag cagttccggg ctggccccca gcaggacgcc accatcagcc agcgggacat
    11641 ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc cagcaatacg ccactatcaa
    11701 tggcggcgtc agtggtgata tcaatcaatc gtgacaacac cactttgtcg acctgcggcg
    11761 tggcggtaag tgcggcggcg cgagcaacca tctgttcaaa cgtggtttgt tcaggaattg
    11821 cctggcgttc caccacgttc agcgactggc tgcgggtaaa acggcgggct gaggtctgtt
    11881 tttcctggcg ggagaacgac tgccaggatt cgggaatata cagcgacgaa ggctgacgtg
    11941 gatcgaaggg aatcgccccg accatcaccg gatttttgat gccctgcgct ttggcatcgg
    12001 caaacagcgc ggcgagtttt tgctggaagg gactgtcggg cgaatccccg ttcacagccg
    12061 gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact gcggtacggc gacataaaga
    12121 aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac ttcctcagcc agtgacgtat
    12181 ccatatcatc ctccacaaaa tgataaaggc tttaataatg attatcattt atattttcgg
    12241 tcgctaacct aaaagcaaac gcgcactatg tcaactcttg aggtaacgca atctgcaact
    12301 cggggttgca tctggttgtg gttacaatga aaatgagaag cattattgat ggattcgcat
    12361 aagcgcaatg tgatgtcctg cgccgttctg ccccctctcc cttccagggt gagggctggg
    12421 gtgagggtta atgttcgcac cagtgctggc tgttcccctc accctaaccc tctccccaaa
    12481 ggggcgaggg gacggattgt gcgctttgtc gaatttgtca ttacgccctt aaccttatta
    12541 ataacaggaa gctgatttgt gagactcgcc ccgctctacc gcaacgccct tctattaaca
    12601 ggacttttgc tttcaggaat agccgcagtt caggccgctg actggccgcg tcagattact
    12661 gacagccgtg gcacacatac actggaaagc cagccgcagc gtattgtttc caccagcgtc
    12721 accctgaccg gctcactgct ggcgattgat gctccggtga tcgccagcgg cgcgaccacg
    12781 ccgaataacc gcgtcgcgga tgaccagggc tttttacgcc agtggagcaa ggtggcgaaa
    12841 gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg ccgaagccgt tgccgcgcaa
    12901 atgccggatc tgattttaat tagcgcaacc ggcggggatt cggcgctggc actgtatgat
    12961 cagctttcca ccatcgcccc gacattaatc atcaattacg acgacaaaag ctggcagtcg
    13021 ctgttaacgc agcttggcga aattaccggg catgagaaac aagcggcaga gcggattgca
    13081 cagtttgata agcaactggc agcggcgaaa gagcaaatca aattaccgcc gcagccggtc
    13141 actgccattg tctataccgc cgctgcacac agtgccaatc tctggacgcc ggaatcagca
    13201 caagggcaga tgctggaaca actcggcttt acgctggcga agttgcccgc agggttaaac
    13261 gccagccaaa gccagggcaa acgccatgac atcattcagc ttggtgggga aaatctggct
    13321 gctgggttaa atggcgagtc actattcctg ttcgccggtg atcagaaaga tgccgatgcg
    13381 atttatgcca atccactgct cgcgcacctt cccgcagtac aaaacaagca ggtttatgcg
    13441 ctgggaaccg agacgttccg tctggattac tacagcgcca cgcaagtgct ggaacgactt
    13501 aaggcgctgt tttaagcatt aactgtcaga cgctgtcacc tgcggcggcg tctggcgaaa
    13561 acgtcgcaac tccaccagca ccagcaataa caacacgccg ataatcaaca aaccaaaacc
    13621 gcttgcgctt gcggaagcaa ccggtgtcat gatcgcgccc aaaccaccca gcagcgccgc
    13681 gcctatggca tcgcccgtca cgttctgcgc cgtccacaaa ccgttaatcc gccctaacat
    13741 cgcttccggg gtttgcgttt gcagcattgt atattgcagc aacgaactaa ccgcgctcag
    13801 ccagccgaac agcgccagac aaaccacgcc taaaatccac atcggcatca ggccaaacag
    13861 accaatggcg aggaacgatc ccagcgtgga gagcagcatc aataaccctg gtcgcgcact
    13921 atgtgccagc ttcccgctgg ttaacgcacc aatagccgcg ccgagcggga tcgccgcgta
    13981 gagaaaacca atctgtgccg ctgacatctg ccagttgtca gccagcgccg gatacagtac
    14041 ccgcaccgcg ctcgccatcg tcaataaacc acccagcagc gcaatcccgc ccaccagcgg
    14101 gctggcaagc agaaaacgaa atcctgccag taatgatttc aacggatgct cacgcggctg
    14161 cggtggcggt ggcaacgccg gaaggcttaa caacggtagc aaggtaataa acgtgcccgc
    14221 cgccgccagc ccgtagttcc aggctacgcc accggtcgcc agcaataaac cgccaatcat
    14281 gggcgaaatc accgacccca gacgcacggt caacatggtg atcgccccgg cctgcattaa
    14341 gttttcacgc cctaccagtg ctggtgtcgc cgccaatagc gccgtaacgc caagcgatgc
    14401 gaaaaaacca tcccataaac caagtaaata gattgccagc aatgacggct ccggcagcag
    14461 tgcattaagg cacagtccaa tgaagccaat gccacaggtg ccgcgcgcca gcaaaatcac
    14521 ttttttgcgc tcatagcgat ccgccagcac accgccaacc atcaggccaa caaacatcgc
    14581 gccgccggtc agcgtcaccg aaagccccac ctgccaggta gaatgcgtca tcatctggat
    14641 ctgcaccggc accgcgacgc cgagcaaacc cagagacaca attgagataa aacgagcgag
    14701 gaatactgcg cgaaacgccg ggtgcgtttt caacaggctg aggttaagca gccaggattg
    14761 tttattcatt acaatgcctt gccatcaaat attttaaata cctattctta ggctgcacat
    14821 gctaacatat ccaaataaga tcgataacga taattaattt cattatcatg gaagttcgta
    14881 tgtctggttc tgttgccgtg acacgcgcca ttgccgtgcc cggattgctg ttattactga
    14941 ttatcgcgac ggcattaagc ctgctcattg gggcaaaatc actccccgct tccgtagtgc
    15001 tggaggccct ctccggcacc tgccagagcg ccgactgcac catcgtgctc gacgcgcgac
    15061 tgccgcgcac ccttgccggt ttacttgcag gcggcgcgct tggccttgcc ggggcgttga
    15121 tgcaaaccct cacccgaaat ccacttgccg accccggctt gcttggcgtg aatgccggag
    15181 ccagctttgc cattgtgctg ggtgcggcgc tgtttggtta ctcatccgcg caggaacaac
    15241 tggcgatggc cttcgccggg gcgctggtgg cctcattgat tgttgccttt accggcagtc
    15301 agggcggcgg gcagttaagt ccggtgcgtt taaccctggc gggcgtggcg ctggcggcgg
    15361 tgctggaagg gctgaccagc ggcatcgccc tgcttaatcc tgatgtctac gatcaactgc
    15421 gtttctggca agccggttcg ctggatattc gcaatctaca taccttaaaa gtggtgctga
    15481 tcccggtgct gatcgccgga gcaaccgcgc tattactgag ccgcgcgctg aacagtttaa
    15541 gcctcggcag cgacaccgcg acggcgctgg gcagtcgcgt ggcgcgcaca cagttaattg
    15601 gtctgctggc gattaccgtg ctttgtggta gtgcgacggc ggtagttggc ccgattgcct
    15661 ttatcggcct gatgatgccg cacatggcgc gctggctggt aggtgccgat catcgctggt
    15721 cgctgcccgt cacgctactt gctacccctg ccctgctgct gtttgccgat atcatcgggc
    15781 gggtgattgt gcccggcgaa ctgcgcgttt ctgtggtcag tgcgtttatt ggtgcaccgg
    15841 tgctgatctt cctcgtgcga cgtaaaacgc gaggtggcgc atgatttacg tctctcgccg
    15901 attaatcatc acctgtttgc tgttggtttc cgcctgtgtg gttgcaggta tctggggatt
    15961 acgcagcggt gccgtcacgc tggaaacctc gcaggtattc gccgcgctga tgggcgatgc
    16021 gccgcgcagt atgacgatgg tggtcaccga atggcgttta ccacgcgtgc tgatggcgct
    16081 gttgattggc gcggcactgg gcgtcagcgg cgcgattttt cagtcgctga tgcgtaaccc
    16141 gctcggcagc cctgacgtaa tgggctttaa caccggggcg tggagcggcg tgctggtagc
    16201 gatggtgctg tttggtcagg acctgacggc tatcgcgctg gcagcaatgg tgggcggcat
    16261 tatcacttcg ctgttggtct ggctgctcgc ctggcgtaac ggcatcgaca cctttcggtt
    16321 gattattatc ggtatcggcg ttcgtgccat gctggtggcc tttaatacct ggctgttgct
    16381 gaaagcgtct ttagaaacgg cgctaacagc gggtttgtgg aatgcaggat cgctcaacgg
    16441 cctgacgtgg gcaaaaacct cgccttccgc acccatcatt atattgatgc tcattgccgc
    16501 cgccttactg gtacgccgga tgcgcttgct ggaaatgggc gatgataccg cgtgtgcgct
    16561 gggcgtcagc gtcgaacgtt cgcgtctgtt aatgatgctg gttgcagtgg tgcttaccgc
    16621 tgcggcaaca gcgcttgccg ggccgatttc ctttattgct ttagtcgcac cgcacattgc
    16681 ccgacgcatt agcggcaccg ctcgctgggg gctaacccag gcggcgctat gcggggcgct
    16741 gttactgctg gcggccgatc tctgcgccca acaactgttt atgccgtatc aacttccggt
    16801 tggcgtcgtt accgtcagcc tcggcggtat ttaccttatc gtcttgttaa ttcaggagtc
    16861 tcgcaaaaaa tgaccgaatc agtagcccgt ttgcgcggcg aacagttaac cctgggatat
    16921 ggcaaatata ccgttgcgga aaatctgact gtagaaatac ctgatggtca cttcacggca
    16981 attatcgggc caaatggctg cggtaaatcc acgttactgc gtaccttaag ccgcctgatg
    17041 acgcctgctc atgggcatgt ctggctggat ggcgagcaca ttcaacatta cgccagtaaa
    17101 gaggttgcac gccggattgg tctgttggcg caaaatgcta ccacgccggg cgatatcacc
    17161 gtgcaggagc tggtggcgcg tggacgttat ccgcatcaac cgctgtttac ccgctggcgc
    17221 aaagaggatg aagaagcggt aacgaaagca atgcaggcca cgggaataac tcatctggca
    17281 gatcaaagcg tggataccct ttctggcgga caacgccagc gagcgtggat cgcgatggtg
    17341 ctggcccagg aaacggcaat tatgctgctc gacgaaccca cgacctggct ggatatcagt
    17401 catcagattg atttgctgga gttgttaagc gaactgaacc gcgaaaaagg ctataccctg
    17461 gcggcggtgc tgcacgatct taatcaggcc tgtcgttacg ccagccattt gattgcattg
    17521 cgggaaggga aaattgttgc tcagggagcg ccgaaggaga ttgtcactgc tgaactgatt
    17581 gagcgcattt atggtctgcg ctgcatgatc attgacgatc cagttgccgg aacgccgctt
    17641 gtggtgccgc tcggacgaac ggcaccttca accgcaaata gttaaactaa gtggtctgcc
    17701 atcatggcat cctgttttct ggatgccatc gcatagcgca acagcacgcc accacaagcc
    17761 accatcccgc cgatcaacgc ggaaaggatc acaataatcg ccttacccgg accgtctttt
    17821 ttcactggca atgacgggct taactgatat ttaaacggcg taaaattcac atcgttgaca
    17881 tgtgcttttg ttaattgctc gacaagatac tgccgattac gtaattcacc gttcagttcc
    17941 gcaacgtcag tgaccgcttt ttctatttcc agtttgcgtt caataccgtc tgcaccgaga
    18001 gaaatagaaa aatcggggtc atctttaacg gcctgaccat tactgtaaac gggcttttta
    18061 attcctgccg cgttggcaat gtcgagtgaa taattgaggc gctgaatgtt tgcatcaagt
    18121 tgatttttcg ttttaatgcg atcctgagcc agtttttctt tttcaaactg ggttttgatc
    18181 tccagtttat tacggacgtt ttctagcgac tctttcacca ccaacgtaga gatataatcg
    18241 atatacccgc tcaaaacggt ctgcgcctct tcactggttg gcgcggtaaa acttagcgtc
    18301 caggaggtat acagtgacgg ttcatctttt ttcttactgg cattgtcatc aaccgctttc
    18361 attttttcgc tcaatgcgac aattgcgcga tgcaaatcca gttcgtcgat tttcgcctct
    18421 tttaattggt ccatcacata aggtgatgaa cgcaggtact cttccagcaa gctaaccgac
    18481 tgaaacttct tgataaacag gttaaatgct tctgtacgat caattttgat atccagatcc
    18541 agcactcgaa gcttagtgaa tgttttctcc agctcttgcc actgaacagg ttctggaggc
    18601 gtgacaaccg ccgcgctggt ccatttttgc ggcaggatga aagagatcag caagcctgcg
    18661 caggcaaacg caaaaatgac cgccatgacc gtttttttgg cccgccataa aactgcgatt
    18721 agattaagta aatcaatttc attattactg ggcgacgcca gaggatactc aggaaaatga
    18781 gcgtcacttc cctgtttaat attcagtgat gacatggggt ataacctgaa aatgagtcca
    18841 ataggggaaa tctccacggc gctcacttta ccagttggtg ataattatcc gaagctgaag
    18901 tttgtaaatt ccttccactg aatgcaacat tcactc
//
LOCUS       DAFNBJ010000095        18935 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000095 DAFNBJ010000000
VERSION     DAFNBJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.095"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..202)
                     /locus_tag="L2X49_003116"
     CDS             complement(<1..202)
                     /locus_tag="L2X49_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HBN4798157.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIG"
     gene            complement(327..2327)
                     /locus_tag="L2X49_003117"
     CDS             complement(327..2327)
                     /locus_tag="L2X49_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HBN4798158.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCTTAQPTEKEE
                     AWDPAIWLEELATLPDATRKRAQALVGKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFANDEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHVRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2324..3460)
                     /locus_tag="L2X49_003118"
     CDS             complement(2324..3460)
                     /locus_tag="L2X49_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBN4798159.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3453..5732)
                     /locus_tag="L2X49_003119"
     CDS             complement(3453..5732)
                     /locus_tag="L2X49_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798160.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFFSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DANFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5743..6831)
                     /locus_tag="L2X49_003120"
     CDS             complement(5743..6831)
                     /locus_tag="L2X49_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBN4798161.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            complement(7138..7455)
                     /locus_tag="L2X49_003121"
     CDS             complement(7138..7455)
                     /locus_tag="L2X49_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798162.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            complement(7516..9401)
                     /locus_tag="L2X49_003122"
                     /pseudo
     CDS             complement(7516..9401)
                     /locus_tag="L2X49_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057906.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            complement(9663..10907)
                     /locus_tag="L2X49_003123"
     CDS             complement(9663..10907)
                     /locus_tag="L2X49_003123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798163.1"
                     /translation="MIGEHKPSIPARTMAALVCIAQNDSQQLLDEIVQQEGLEYATEV
                     VIARQFITRCYESDPLLVTLQYQDEDYGYGYRSETYNEFDLRLRKHLSLAEESSWQRC
                     ADKLIAALPGIPKIRRPFIALLLPEKPEIANELASLESSRSSLHSKEWLKVIATDNTA
                     VKKLERYWGLDVFSDREASYMSQENRFGYAACASLLREQGLAAVPRLAMYAHKEDCGS
                     LLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFPHATLAALAELLALKEPPARPGY
                     PIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAEVMPWLSTQARAVLNSYLSAPPK
                     PVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPLELTPQIYWQPGEQERLAATESA
                     RYFSTESLAERMEQKSGRVVLQ"
     gene            complement(10904..11147)
                     /locus_tag="L2X49_003124"
                     /pseudo
     CDS             complement(10904..11147)
                     /locus_tag="L2X49_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            complement(11157..14951)
                     /locus_tag="L2X49_003125"
     CDS             complement(11157..14951)
                     /locus_tag="L2X49_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HBN4798164.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKVNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAHKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNDSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            complement(15092..17125)
                     /gene="metG"
                     /locus_tag="L2X49_003126"
     CDS             complement(15092..17125)
                     /gene="metG"
                     /locus_tag="L2X49_003126"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBN4798165.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            17257..18366
                     /gene="apbC"
                     /locus_tag="L2X49_003127"
     CDS             17257..18366
                     /gene="apbC"
                     /locus_tag="L2X49_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBN4798166.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            18629..18909
                     /locus_tag="L2X49_003128"
                     /pseudo
     CDS             18629..18909
                     /locus_tag="L2X49_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
BASE COUNT         4475 a         4839 c         4903 g         4718 t
ORIGIN      
        1 caccaataga agcaaattga attttggttt cagaagattg cttaagcacc ttgtcacctt
       61 tgtagacata ggtaatggca atttcagtgc cgttcagatt ggcgctgaat ttcttcgatt
      121 cttctttgtc accgcagcca gcaagagaga aaaccagaac agatgcaaca acgagggaaa
      181 acagcttatt gaaagccttc atgtaaaact ccattttatt taatcaagaa actggtgact
      241 ctcaccaggg gctatataga atatgcctaa taccgtgacg tgagcagtcc ggaactggag
      301 tagaactctt agtaaaaagc actatttcat ccttgttgct gaagcatggg gaataattgt
      361 tcgcaaagta aaacaccgtt attcattgct tctacccgtg cctcgctttc tgtattacga
      421 aattgtccca acacatgtgc cagccgataa aaaccgaccg cggagaggtc attcgccagc
      481 aactctgcct gactaatagc gctctgttcc tgatagcgcc agccgttatg gagcagttga
      541 ataagtaacg cctggcagcg catcagcaac tgatgagcgg tagacggcac aggcaaaacg
      601 ctggcagaag gtagcggtgc cacaggcgca gtttctgcgt ccagcgccca ggcgcgggtt
      661 tttgtcatca tcacccgtgg ttccagtgtc aattgcccat caacaaaact gacaaagcca
      721 gaaaccagac tcacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg ttcaacggca
      781 aaaggagaac aggcagatgc cggaagggat aacgtcagga gattatcttc accttcgccg
      841 ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga aatacactca
      901 gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca ggcgggggga
      961 cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg cgcgctcaac
     1021 atttgccagg catcaggcga cagcggcaca acgctgctta agcggttgcg ggtagctaac
     1081 agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc acccgccagt
     1141 gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagttttc ctgttcactt
     1201 cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt aaaccagatg
     1261 cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac cagccgcaga
     1321 tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca cggcacagga
     1381 ggaacttcac caacactgtc gcgccgggcc atctcttgtg cgcaatttaa tcgactgttt
     1441 aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg gacgtggaag
     1501 gcatcaacgc ttgcccgtag ctgtcgtagt gattcactca cccatcgcca gttgcagcgt
     1561 tccgccgcct gctgcgcgcg actgaaagcg gcctcgtagt gaataagcgg ctggctgatg
     1621 ccgcccagcc ataatgcctg gcttaattgc tgaacatatt gacgacacgc gttaccctcg
     1681 tcgttggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg ccagattaaa
     1741 tgagtaaatt ccgcttgctg agttttggcc tcgacgaagg cctgcacagc cagtacgaca
     1801 tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact gctgcgggaa
     1861 taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcgcccgg cgtacagaac
     1921 aactcaatgg tgatgccttt accgaccaac gcctgtgcgc gtttgcgggt ggcatcgggc
     1981 agggtagcca gttcttccag ccagattgcc ggatcccacg cttcttcttt ttccgtaggc
     2041 tgggcggtag tacaaagtcg ttgataactt aacacaagca tcacgcgatg acggcacata
     2101 ccgctggccc cgcagctgca ctgagcctct ttcagtgcct ggccgttcgc cagctgggta
     2161 cggacaccgt cactgaaggt ggcgattaaa gcgctgttct catggctgat ttccgggacg
     2221 ttgccatttt ccagttcctt aagactgcgc ttaacaaaac cggcattgct taacgccgtc
     2281 agggcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac tgaagattct
     2341 ccgcaagcca tgatgccagc tcgcccggtg tcatggcggc tatttgtgcg ccgacattta
     2401 ccagcgcctg ggccgtatcg tggtcatagc aaggtgttgc tgtgctatcg agcgctgcca
     2461 gtcccagcac tttgatgccg ctctggacac actttttcac ctgatgcgtc agtaatgatg
     2521 atgaaccccc ttcgtaaaaa tcgctcacga ggataatgac gcttttcgct ggttgttcaa
     2581 taagttgccg accatactcc acggcactgg cgatattggt cccgccgccc aactgtactt
     2641 tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaacgacg cttgtgtcaa
     2701 acgccaccag atgggtacga atgccgggta actgccacaa acaggccgcc atcaccgcag
     2761 agtggatcac cgagtccacc atcgatccgc tttgatcaac cagtaagacc agttgccatt
     2821 gttcgctttg gcgtttaatg cggctgttaa agcgggggga ttcgatatac aacttgccgt
     2881 gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca aagtttcgcg
     2941 ccagtggaat taatgagcgg cgacggcgat cgcggacacc agaaaaagcc tgacgaactt
     3001 cctttgccag tcgcgccata atttcttcaa caacctggcg cactatctgg cgggcggcag
     3061 ccagtacttc gggattcatc agatgtttgg tgtgcaaaac ggcgcgtagc aggctttccg
     3121 aaggctgcat acgttccagc acgtcgagat ttgtcaccac atcttcaatg ccgtagcgca
     3181 gtacggcatc gctttccagc cgctcaatca cctgttgcgg aaacagcgtg tggatactgt
     3241 tgatccactc aggagtggtg agatttgagc cacctaatcc accagagcgt tcaccacgct
     3301 ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtgatctatc tgccgggcgt
     3361 tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat cgccagcgtt
     3421 gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc ccagtgttgc
     3481 agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg cggtggacaa
     3541 tgcaacggca tttgcagtgc cgaaacggga agttgcgcca gttgataatg ctcaagcacc
     3601 tgatgcgcca gcgttccgcg ttctcgtggc ggtagccagg ccatcgccgc gcgtaaatcg
     3661 ggcagggcat tgataaaatt ggcatcactc agttgattca aatggctgct gaaaccggcg
     3721 ataaatgcag gttgacaggc cagttgatga cgggctaatg ccagcaaacc gtgcagcgcc
     3781 tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc ggcactggcg
     3841 ttgggatgtt ccagacgcat tagtgcgcca agagccgcgc cgcgatccag cgccggggca
     3901 tgaatcgcct gactgcgtcg ctccagtaag gctaccgccg cagaaagcga aacgccggat
     3961 aaattaacgc cgctatgtag atcgcgcaga atatggcaaa gcgcttgcca gctatgcagg
     4021 tgagcgtgaa actccttttc atccggtctg ccgttagatt cacacagcca cagcgtgcga
     4081 tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc gctaatttca
     4141 tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc aaattgattt
     4201 tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga cgccagaccc
     4261 gctaacgccg cctggctaag acatgtggtg atactgccga ttccacccgc gtccagcata
     4321 tcggcttcta atttatggcg tgcggcttcc tgaagtgttg caccaaagca tgcggcctca
     4381 atcaatgccg catgctggct aagcgggcgg gttaatttcc agtgttcttc accgttgcct
     4441 gcaagtgtca gtgtacttcc ctgctggcgt acaatcccag ggatctccag tattgccagc
     4501 cgatgtaaca cctgactttg cgccagccca tcgggggtaa agcgatttaa ggtaagctcc
     4561 gccggaagag agattgcggt acgttccagt tcgcaggtga catcttttgg cagaggcggt
     4621 tgtggcgtgg aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc tatcaacgtt
     4681 agcagaatcg gatcggtatc gggatgaata acgccgcgat agctccacgg caacggtgca
     4741 ttcagggctt ctttaatcaa cgagcctgct atggcatcca gccagtcagt gcgtaacggt
     4801 agtgtatgac cgcgcaactg tgccagtgcc attgcatgca gatgtgcggc agccatatcc
     4861 gcggtcgaag caggcaagtt gtgctggcgc aaacgggtga gaaccgtttt tagcagttgt
     4921 tcaccggcct gctgtaagcc ccactgccag caccagtttt gccagaccgg ggcaggcata
     4981 ccggaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggcgt gagataacaa
     5041 cctgtaatgg catctgccag cgagggcaat tccggcgtgt taatgtcctg cgggcattcg
     5101 cgccacattt ttgccagtgc cggagcgtgc cagccaccgc agacgactaa cacgtcgcca
     5161 ttattttgct gcaccgccca tgcaatccag cgagccataa aggcttcgcg ctgacgattg
     5221 agtgcatcgc cggggaaatc gccccgcagt tgggcaaaat agtgcgccag cgcagagggt
     5281 aatgcagttt gctggctttc atcttcgaac aagtgatccc acagggtatc gctgttatcc
     5341 atgcgggtgg cacgcagcag taaggcctgg ctttcatctt gcgtatcagg cgaatcgtcc
     5401 tcttcttcac tctgcgccca gcaaggcaaa tcgatgaagt aagtttgtgc ctgaatacga
     5461 cgtgcggctt gtagcgcctg ccactccggc gaaaattcag caaatggcgt ccaggcacca
     5521 cgcccggggg ctgcaccgtc ctgatactgg caataactgt aaatagctac cggaagctgg
     5581 tgggaaaaaa aaagttcgtc tacccggtca ttaaaatcag ccgggccttc aatcaacacg
     5641 tatcgtggcc gctggctttc gattaacgat ttcaccagcc gggcgcaggc cggactatga
     5701 tgccggatgc cgacaattaa cggctcgctc atgcatcttt cctcacggca ggcggtggcg
     5761 ggcttgataa taagcctgcc agtgagcttc tttatgtgtg gcgactcgtt gttcgaagta
     5821 acggcgcaga cgagcgcgat cttcttcgtt atctttgacg atggttccgg caatgcagtc
     5881 caccaggtcg gctggctcac ctgcgcgatt cgctaaaaac caggcgcgga cgcctacggc
     5941 gtgggccacg ttcacggctt cagcggtgga cataatcgct gttagtgtat ccattgacgt
     6001 tttcttttcg ccgttggcac gcagatcgcg gaaggtgcgt accagtaact ccagtactgc
     6061 gtcaggcact ttatgcggaa taccgctatg ggccagcaaa cgcgccgaag cactggcgac
     6121 cagttccagc tcctgggcga aatccataat cggaaagacg gtttcaaaat cgaagcgacg
     6181 ctttagcgcg gcactcatct cattgacgcc gcgatcgcgg gtattggcag tggcaataat
     6241 attaaagcct tcccgcgcat agagctgact ggcttcaccg gtgagttccg gcaccgtcat
     6301 caccctgtca gaaagcatac ctaacagaca gtcttgtact tccagcggtg tacgcgttat
     6361 ctcttcaaaa cggacgattt tgccgtcgcg cattccctgg taaagcgggg cagggaccag
     6421 tgcttccgtt gacggaccat ggttgataag cagggcgtag ttccagccgt acttgatttg
     6481 atcttcggta gtagatgcgc cgccctggat ggttaacccg gcatcgccgc taatagcggt
     6541 tgccagtaat tcagacagga gagattttgc ggtgccaggt tcccccacca acatcaaacc
     6601 gcgcccggtg gcaagagtga ctaacatgcg ttcgacagcg gagggactaa tcaccacttt
     6661 acggctaata ttttgcgcgc tatcgccaag gataaaagca cgggccgcag gcaaacttaa
     6721 ctgccaaccg ggcgggcaag gagcgttatc attttgtttt aattttgcca gttcatcggc
     6781 gtataacaca gcagcaggcg gacgctgcag atgattattc tgcggtgaca tatattcatc
     6841 cctgaaatgt attaatgaaa taaacgatat tttacgcgca ataaatgttt ggcgcattag
     6901 ttgtttgagc aaaaaatatc tggaaagcag atcgcagtaa ataaaaaaac ctgctggtga
     6961 ggcaggttct tttatttact attttcaggc ctaacaccac agcctgtgca acatcgtgtt
     7021 ccaacgaacg aattataacc acggctggac cccgttgcaa ttaagataat gagatttata
     7081 atccagaccg ttgggacgtt gcgccggatc ggaccaattt gaattattta gagtatatta
     7141 ttctatttct tggttgaata tattctgtag ttcacgatta aaaattgcta ttacagtttc
     7201 aggtgattca cctcgtaatt tccgctcatc aatacactca tctcttatat aaatcaacgc
     7261 atcatttatt agcgggtcag catcttctcg atcacgttga tgaaatgttg gacgtagccg
     7321 gaatcgctcc agtcgataga cggaacctgt aaataattca gagcatttat ggatctcaag
     7381 tctgaaggaa aacggatctt caggtacagg gacctcataa tcgtaaatag caaccagctc
     7441 tttttgcacg atcatcgctt tccccgctgg taagtcgtgt tgataacggg cagttgcatc
     7501 actgcccgct gtgttttaat ggaaaatgac gtcaggtgcg cttaacgcct cactcaaatc
     7561 aaacacatca agttcggcaa aagtgacgga tttattgtca ctgtacactc gaagagaacg
     7621 gaagctcatt agcgctttgc tatcaacatt gtagtggtta aaaccgggtg aaaacttaag
     7681 gaccagccgg tactcaccca ggtcatagac aaatgcgtcc tcatagcctg actcccagcc
     7741 tttgtatcgc attcccataa gttgcccaac ggtagcagat ttaccttccc agcgattcaa
     7801 actattactg gctgactcgt caggcgtgag cagcaccgtg cgccgtgtca actggcgaag
     7861 tggcggtata atttcgtaat cggcaaaaag ctgggcaaat tcactgcgca tttctgctgt
     7921 catttctaac gggtgggcaa tgccaatcag agcatccgca ggcaggtcaa ttggctcatc
     7981 ctgcgcattg cagaactccc cctctgcggc cacacgaaag gcgttgagta aacgacgcgg
     8041 ttcatttgcc ggatacacgc cccatattaa tcgctgggta accaggcggg taaagggatg
     8101 attaacaaac agagtatgaa aatctgccag cgaccagcgt cgggtggtac gtagggcagc
     8161 ttcaagacgc gggagcaggt ttttgctcac ctgagtagca tcttttttag cccttttagt
     8221 cgggccagtg cctcgggcgc tttcagttga tcgtcatcgg cgcgcaaccg gggaacgctt
     8281 ttctggcgaa cgttttgctg atcaaagata accgggttga ggttttcatc gaagcgaacg
     8341 gtaaactgcc ggggaccaaa atcaaaactc aacgcctgcg gatcatcaag gcccagggtt
     8401 gggactaatc tgtccgctag ctcttcctgg cttagcccgc gattttctgc gaccacctga
     8461 agaaattccg ccgcgttatc acgtaagggg cgggatttag cccgttgcga tatatgatgc
     8521 agctgtatca gcgccatatc attattcatc agggtaagga tgttcaggcc gctgacagca
     8581 cgggcagatt tgccttcttg tggccaggcg aggatctgtg tcgtcagatc ccgtgccgtg
     8641 ctttcgtcac caaagagact taacgccaga aatgcccagt tgtcttttgc gggggcacca
     8701 gcttgttgcc acgcagtgaa gagatcccag gcaaaagctg ccagcgtttg tggctggcag
     8761 aacgttttca gttgttccag cccgctataa aaacgtcccc cctgggtaaa gcgcagcatt
     8821 tcgccgataa tttccagagc gtcaccggta acaggctgat tattagtgat aagtcgaggc
     8881 ctggaccaca ttgccggatg ccagaaatca ggggcttttg gaatgcgtgc cgggtaaatg
     8941 tccattggac cctgtttaag caactgctcc agggcagacc atacatctgt acgctgccag
     9001 cggtttgcca cggtttgtag caattcgcca tgtccctgtt cgtacagtaa acgcagggca
     9061 agtaatgcgg cttcgctatt atcgctgggt ttggtaaaga caagagggat aagtgcacta
     9121 gccgtatgtt ccggccattg taaaatccac tggcgagcca gatcacgctg cgccgcgaaa
     9181 cgtcgccaga cgcgggcaac aggcagcgcc agttctgttg cgccaaaatt taaaattaac
     9241 ggaaatgttt cttttggacg atgtgaaaaa gccaacagca gacctggtag cgcatccaat
     9301 ccgaggatac tgagaaaata gtcctcaccc gcgaacagat gctttttctc aatgatacgc
     9361 agccagaagg tcaaagccat ctcacgcggc aactgtgcca ataaatacag gttccagaca
     9421 ctcttttccg gacgataacg tgagaagttg tcattttccc atatgttaat taacgcttct
     9481 atgtgaccgc tgcggagcgc ctgttctgct aattgtgctt gcggggagtt ccagccattc
     9541 aggatctctt caacccgccc ctggtagtaa tgccactgaa caatgagtga attgcgtgca
     9601 gcatccagtt ttccggggag tatataattc agaaacagcc atacatcatc cccaaaaccc
     9661 agttactgta atacaactcg tccacttttt tgttccatgc gttccgcaag agattccgtg
     9721 ctgaaataac gggcagactc agtggcggca agcctctctt gttcgcctgg ttgccagtaa
     9781 atttgcggag ttaattcgag cggtgccaaa tcaagacgtg gagctgtcat ttttttctta
     9841 ctacgccacg gtggtgaaac gagcatttca ggcagactgc tgttatcggt gctatcaata
     9901 accggttttg gcggtgccga taaataacta ttcagcactg ccctggcttg agtacttaac
     9961 cacggcatca cttctgctgc cagttgtggc tgcgatgcca tcagcgtttg taacagcgta
    10021 cgccagtatt catcgcgtgc tttttgctgt gcaggcagct ttttgtcttc gatgattgga
    10081 taacctgggc gggctggtgg ttcttttaac gccagcagtt ctgccagtgc ggcgagcgtc
    10141 gcgtggggga agtttttact gtatttagcc acacgttgca ggctgggttt gtttttatca
    10201 gccaccagta gcaaggtgcg gatgacttgc ggatggttaa tttgtaccag cagactgccg
    10261 caatcttctt tatgggcata cattgccagg cgcggaacgg ctgccagtcc ttgttcgcgc
    10321 aaaagggagg cgcaggccgc gtagccgaag cggttttctt gtgacatgta gctggcttcg
    10381 cgatcgctaa atacgtccag gccccagtat cgttcgagtt tttttaccgc cgtattatca
    10441 gtagcaatga cctttaacca ctcctttgaa tggagacttg accgggagct ttcaagactg
    10501 gcgagttcat tggctatttc aggtttttcc gggaggagta gtgcaataaa aggacggcga
    10561 attttgggta ttcctggcag tgcggcaatg agtttgtcgg cgcaacgctg ccagctgctt
    10621 tcctctgcca gagagagatg cttacgcagt cggagatcga attcgttata ggtttctgag
    10681 cgataaccat agccataatc ctcgtcctga tactgcaatg tcactagcag aggatcactc
    10741 tcataacatc gtgtaataaa ctggcgtgca atcaccacct cagtcgcata ttccagtccc
    10801 tcttgttgga cgatttcgtc gagcaactgt tggctgtcat tttgcgcgat acagactagc
    10861 gccgccattg tccgggccgg gattgagggt ttgtgttcgc cgatcaagtt gatggcctgc
    10921 gtgatggcgg actgccactc cagtgaaaaa ttttcataac gccagacaat gttttttttc
    10981 ttctcagctt tttgtagcaa tttatcaccc agtgtctgaa tgatgctttc agtgataaca
    11041 ggcaccggtt cgcccggcca tttgcgatga ctgagtggtg ttttgccttt tttatatttc
    11101 agttccagtt gggcgttatc cgccagccac ggtaattcct tgtccatgct ctttccttat
    11161 tcaaacaggg cttcaatatc gttaattagc tcgctggcgg tgatggcatc gagtacggag
    11221 aactgcgctt cctgtgttga attactcccc cagccatagc tttccgcttt gccttcccat
    11281 aaccacagct tgcttaagag ctgctcagcg ctaagttcgg ctggtgacat acccaccgca
    11341 aaaccttcgt cgatttccat gattaacgac cagcgaccca aaggtttgat catccatccg
    11401 atccagcctc catcctgtgg ttcgcccttt atccagcctt tattcgccag ccccatgacc
    11461 cgaccactcg ggcattttct gcctgcccag cgggtcagtt cactggcatt gcgctcggct
    11521 tctgtcaggg cgtagctgtt acggtcgagc tggcggaacg gtggtagcag ttcgtagtcg
    11581 gcaaaaagct gaccaaaggc ggcagcatcc gttggtgata tttccagaac gtgaggagtg
    11641 ccgatagaga tatcgccttc cggcagggtg aaaagatcat cgtcagcggt gctgtagctg
    11701 ttatcttctg ctacgcgaaa gcaagccagt agctggtttt cggcgctata aacgccccaa
    11761 atcagacggc gggttaagtg acgaaccagc ggatgctcaa ccaggaagag ctggaagttt
    11821 tccagcgacc agcggcggcg caggcacatg gcggattcca gccttgctac ctgctgggcg
    11881 gcgatggtac gcgcatcttt tttcagcaat ttgtagcggt taaccgcatc gttcgcccgc
    11941 gtttcatcat cgcttttgtt cggtttgggc aggtctttca ggcggctgcc ggaaacatca
    12001 cgcacaaacg gttttaaggt ttcatcaaag ctgacggtga actgccgtgg gccaaaatcc
    12061 agcagcagcg aatcgttatc atccagaccg agatccggtg ctaaccgatc ttcaagctcc
    12121 gccaccgtga gttcgcggct ctcggcaatg tcggcaattt tttcttttgc ccgctcctgt
    12181 aatgctttga atttcagttt atgggcgatg ccgttaagct gcataagggc gatatcacta
    12241 ccgatagcag cgagaatatc caacccaacg gtggcgcgtt tatgctggga ttcaccaggc
    12301 caggcgcgta ttaatggcgt cagtttgcgg gcggtgtcat cgttaccgag aacgcctaac
    12361 gcagtgaacg cccaactctc tttcgacggc gcgccagcgg tctgccaggc ggtaaacaga
    12421 tcccaggcaa atcccgccag tgagtcggcg gaacagacgt ctttcacctg caataatccc
    12481 ggatacagag cctcttcctg agggaagcgg agcatttcac cgaggtggag gagggcgcta
    12541 tctggcagtg attgggcatt tgcttttaat accgggcgcg tccagagcga tggctgataa
    12601 aaggccggaa gagtggggat ttttgtcggg tgattatcta agggatcgag cgcaagcaga
    12661 gcgttcaccg catcggttac ttccggctgg ttataacgtc gggcgatttc ttgcagtaat
    12721 ggctgatgac cgttttcggt aagcatacgc aaggcagcgc gggcgttatc ctgtgcttca
    12781 ccggctttgc cgagcgccgc aggcagcagg ccggtaaggg catgttccgg gtatttcaac
    12841 agccagctac gggcgttttc gcgtagcgtt ttcagtttgt tgaaggcacg ggcgacggca
    12901 ggagccagct cactcgctgc gaagtaattc gtgatgggca aggcttcttg tggatagcgt
    12961 gcaagtgagt ttactaaccc aggtaaccct ttgagtccaa aatgtaacat ggcgtattcg
    13021 gtatcggtat gtggagatag ggtaagggca ttccatgctt ttactgcttt ctccagcggc
    13081 aatgttgtca aaatccccaa ctgccaataa tgcccggtag gtgaatgaaa caccttaaat
    13141 tcacttagta aggttgaata atcttcattt agccatgcct caactaccgc ttcgggagct
    13201 ttaatatatg taccagcgat ccgtcgttga aaacccatcc ttgccaacag gttggtagta
    13261 ctttcttcat ggctaaccgt gatgtgtttt gaataccaga tatatttcgc caaaagttga
    13321 ttactgattt cttctgtcag ataacagatt ggctcaatgc ctaatggcgc taaatccagc
    13381 accggaatcg gcgatttttt ctttttcgaa agccagggag gggagactac tacggctggc
    13441 agtagatcgg cgctggcatg gtttgagggc tgtgtcagtt gttgctggca tgatttcagt
    13501 acggcaactg cgggtgtcga gagccaggga atgacctgtt ctgccagtgc tggttgtgag
    13561 ataagcattg tcattagcat aatgcgccaa ctgttctctt ctttttgccc aagaagttcc
    13621 gtcagtgccg ccattgctgc atgtgggaac gcagcaatgg ctttcgtcat ccgatcgtga
    13681 cagcgtttag tatgcccggc tacacgtata agcagtgtta atgcgaacgg atgattgata
    13741 tgacgcaaca catcggcgca gtagtcactt gcggcatagg gagcaaatcg gggcagggct
    13801 gcaacacctt gctcctgtaa tacggtggct gaccatatcg caccacaata gtaatcatca
    13861 aaaatggcat atggctcgtg atatttttct aatgaggcaa gaatgtgctc atcagtcgcc
    13921 actattttta accactcaag cgagggtaat tttttttgcc ccagtagacg ctgggcaatt
    13981 tcatgtgcaa tttctggttt ttcgggtaat aacaaagcaa ttaatggttg ccgccattct
    14041 ggaatatgtg gaattgcggc aataagtttt tgtgcacatt tttgccagag agactcttcc
    14101 gccagtgata aatgtttacg taggcgtaat tcaaatctgg aatattgctc caagtagtca
    14161 ggtgcaatgc cagacggtag aataataatg acattattcg cataatccca tttaatatca
    14221 atctgttgaa ggtgtattat tacatcagta gcatattcca gaccgccttc ctggacgatc
    14281 tgatccataa catcctcacg cactttatat cttgtgcatt tcatttcaag tgccgtgagt
    14341 acagccatta ctggtgatgg tagctgtgtt gttttcaggc cgtcgatctg actgatcgcc
    14401 tgggcgacgg tgttttgcca ttctaaagaa caggcgctga agtcaaatgt ggctgtttta
    14461 ttatcccgtt gcgacacact acaggctaag cgacgtaatt gggtcaattt gtctgcttgc
    14521 tgaacaggaa ctccgggaaa gcggcgatga cttaacgtgg tgtgtacaac ttcgggcgga
    14581 aaaacgacag caatatcttt cgccagccac ggtaaaacag catctgtatt tttgtcaggc
    14641 gcacgggttt ctgctacgaa agagggttgt tcctgaataa tggtggataa gtccgctacc
    14701 ttgagagagc ccgttaccgt ttgggaaggt tgcaaagaat taaccttcgc ttgctccaca
    14761 taccccttct tcaccttctc cgcaatcagc ttaagctccg cttttgccgc tgccgcagca
    14821 tctgaaaaac tttttatctg gctttgccct ttggtgccaa cttttcccca actgatatgc
    14881 aactcgtttc cctgttgctc aactgcccag aatttatgtg atttttcgtc ctgatagata
    14941 aagtgtctca ttctgcatcc ctgtgatttt atccagttga atgcagatgc taccagtatt
    15001 tatgcgggtt agagagagac aaaatcgcaa gaaaacaact ttaggaattt ataaagaaaa
    15061 ggcgctgtcg atgcagcgcc ttaaaggggg attatttcac ctgatggcct ggtttagcac
    15121 cagcatccgg gcttaacagg aagatatctt tcccgccagg accagcagcc attaccatgc
    15181 cttcggagat accaaagcgc attttacgcg gtgccaggtt ggccaccata atggtgtgac
    15241 gcccgatcag tgcctgcgga tccgggtagg cagaacgaat accggagaag acattgcgtt
    15301 tttcaccgcc gagatccagt gtcaggcgca gcagtttgtc agaaccttca acaaactctg
    15361 cgttttcgat cagcgcaacg cgcaggtcaa cttttgcgaa gtcgtcaaag gtgatggttt
    15421 cctgaatcgg atcatctgcc agtgggccag taactggcgc ggcagctgct ttcacttctt
    15481 ctttagacgc ttccaccagt gcttcaacct gcttcatatc gatgcggtta tacagcgctt
    15541 tgaacggatt cactttgtgg cccagcagcg gttgctggat accatcccag gtcagttctg
    15601 tattgaggaa tgcttctgcg cgctcggtca gtttcggcag taccggcttc aggtaagtca
    15661 tcagcacgcg gaacaggttt atgcccattg agcaaattgc ctgcaggtcg gcatcgcggc
    15721 cttcctgttt tgccaccacc cacggagcct gctcgtcgac atagcggtta gccagatcag
    15781 ccagcgccat gatttcgcgc actgctttac caaattcgcg gctttcccac gcttcaccaa
    15841 tcacttcagc ggcatcagtg aaggttttgt acaactgcgg gtcagccaat tcgcttgcca
    15901 gcacgccgtc aaaacgcttg ttgataaagc ccgcattacg agaggccagg ttaaccactt
    15961 tgttcacgat atcggcattc acacgctgaa cgaaatcctc caggttgaga tcgatatcat
    16021 caatgcgcga agagagtttc gcagtgtagt agtaacgcag gctgtcagcg tcaaaatgat
    16081 tcagccaggt gctggcttta ataaaggtgc cgcgagactt ggacatcttt gcgccgttca
    16141 ccgtcacata gccatgaaca aacaggttgg tcggcttgcg gaagttgctg ccttccagca
    16201 tggcaggcca gaacaggctg tggaagtaaa caatatcttt accgatgaag tggtacagct
    16261 cggcggtgga gtctttcttc cagtattcat cgaagcttac gctgtcgccg cgcttgtcgc
    16321 acagattctt gaaagaaccc atgtagccaa tcggtgcgtc cagccagacg tagaaatatt
    16381 tgcccggcgc gttcggaatt tcaaaaccaa agtatggcgc gtcgcgggag atatcccact
    16441 gttgcaggcc ggattcaaac cactcctgca ttttatttgc cacctgctcc tgcaacgcac
    16501 cgctgcgggt ccatgcctgc aacatttcgc tgaaagaggg cagatcaaag aagaagtgtt
    16561 cagaatcacg cattaccggc gtagcgccag aaaccaccga tttcggctcg atcaattcag
    16621 tcgggctgta ggtcgcgccg cagacttcgc agttatcgcc gtattgatcc ggggatttac
    16681 atttcgggca ggtgcctttc acaaaacggt ccggcaggaa catgcctttt tccggatcgt
    16741 acagctgaga gatggtgcgg tttttaataa aaccgttctc tttcaggcga gagtagataa
    16801 gttctgacaa ctggcggttc tcttcgctgt gcgtcgagtg atagttgtca tagctgatgt
    16861 taaagcctgc gaaatcagtc tgatgctcct gactcatttc gccaatcatc tgctccgggg
    16921 tgataccaag ctgctgagct ttcagcatga tcggtgtacc gtgggcatcg tcggcgcaga
    16981 tgaagttgac ctcgtggccg cgcattcgct ggtaacggac ccagacatca gcctggatgt
    17041 gctccagcat atggccgagg tggattgagc cgttagcgta cggcagtgcg cacgtcacca
    17101 gaattttctt cgcgacttga gtcatagtag gcattacttc ttaatagtga aaaggggtta
    17161 gatagtacca aatgggaaaa tgttaagtaa gtacttctct atattcttca ggtaaactat
    17221 cagctgatgg tttatctata caaaaaagga gtcgggatga acgaacaatc ccaggccaaa
    17281 tcaccagaag ccttgcgcgc aatggtcgcc gggacactgg ccaattttca gcacccaacc
    17341 ctgaagcata acctcaccac gctgaaagcg ttgcaccatg tcgcctggat ggacgacacg
    17401 ttgcatgtgg aactggttat gccattcgtc tggcatagcg cgtttgaaga gttaaaagag
    17461 caatgtagcg ccgaactgct gcgtatcacc ggggcgaaag ctatcgactg gaaactgtcg
    17521 cacaatatcg ccaccttaaa acgcgtgaag aatcagccgg gtattaatgg tgtgaagaac
    17581 atcattgcgg tcagctccgg taaaggcggc gtgggtaaat cctccacggc ggtaaacctg
    17641 gcgctggcac tggcggcgga aggggcgaaa gtcggcattc tggacgccga tatctacggc
    17701 ccatcaattc ccaccatgct gggcgcagaa aatcagcgtc caacgtcgcc ggacggtacc
    17761 cacatggcac cgattatgtc tcatggcctg gcaaccaatt caattggtta tctggtcact
    17821 gacgacaatg cgatggtgtg gcgtggaccg atggcgagca aggcgttaat gcagatgttg
    17881 caggaaacct tgtggccgga tctcgactat ctggtgcttg atatgccgcc aggcaccggt
    17941 gacattcaat tgacgctggc gcaaaacatt ccagtaactg gtgcagtcgt ggtcaccacc
    18001 ccgcaggaca tcgcgttgat cgatgcgaaa aaaggcattg tgatgttcga gaaagtcgag
    18061 gttccggtgc ttggtattgt cgagaacatg agtgtgcata tttgcagtaa ctgtggtcac
    18121 catgaaccga ttttcggtac cggtggtgct gagaaactgg ctgaaaaata ccacacacag
    18181 ctgctggggc agatgccgtt acacatctcc ttacgtgagg atctcgataa aggcacgcca
    18241 acggttatca gccgtccgga gagcgaattt accgctatct accgccagct ggctgaccgc
    18301 gttgcagctc agctctactg gcagggtgaa gtcattccag gcgagatttc cttccgcgcg
    18361 gtctaacgcc tattaaccat tctggttatt cttcatacac cgcagagatg ttacattgat
    18421 gcggtgtatt agtttttgcc gcattaatga acacttattt aatactgcga atattctgcg
    18481 gaccttcgtc cgaaactcta caaagtcgat tgatcataca ggcaatgctt cattatcatc
    18541 cttcgtggct gtgtagtgga tgtacagcta tagtttgtta tgcactcatt ctatccaggc
    18601 ataaccttta ctacagggat aacgttcaat gaataagtat tggctgtcag gaattatttt
    18661 tcttgcctat ggattggctt ctcccgcatt ctcttcagag actgccacat tagcgattaa
    18721 tggcaggatc agtcccccaa catgcagtat ggctatggtt aatggtcaac ctcaacagca
    18781 ttgtggtcag ttaacgtata tgttgacacc cgtcatctgt tttcttcgcc cgtcaaaggt
    18841 gtaacgacag aagtggttgt tgcaggtagc gatagcaaac gtcgaatcgt gttaaatcgc
    18901 tacgattaat ttggcaacct gattttaatt gcatt
//
LOCUS       DAFNBJ010000096        18720 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000096 DAFNBJ010000000
VERSION     DAFNBJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.096"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..687)
                     /locus_tag="L2X49_003129"
     CDS             complement(<1..687)
                     /locus_tag="L2X49_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBN4798167.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKR"
     gene            complement(698..1699)
                     /gene="ltaE"
                     /locus_tag="L2X49_003130"
     CDS             complement(698..1699)
                     /gene="ltaE"
                     /locus_tag="L2X49_003130"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBN4798168.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKDLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1736..3454)
                     /gene="poxB"
                     /locus_tag="L2X49_003131"
     CDS             complement(1736..3454)
                     /gene="poxB"
                     /locus_tag="L2X49_003131"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBN4798169.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3587..4555)
                     /gene="hcr"
                     /locus_tag="L2X49_003132"
     CDS             complement(3587..4555)
                     /gene="hcr"
                     /locus_tag="L2X49_003132"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBN4798170.1"
                     /translation="MTMPTNQCPWRMQFHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4567..6219)
                     /gene="hcp"
                     /locus_tag="L2X49_003133"
                     /gene_synonym="priS"
     CDS             complement(4567..6219)
                     /gene="hcp"
                     /locus_tag="L2X49_003133"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBN4798171.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6363..7262)
                     /gene="lysO"
                     /locus_tag="L2X49_003134"
     CDS             complement(6363..7262)
                     /gene="lysO"
                     /locus_tag="L2X49_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBN4798172.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7757..8452)
                     /gene="aqpZ"
                     /locus_tag="L2X49_003135"
     CDS             complement(7757..8452)
                     /gene="aqpZ"
                     /locus_tag="L2X49_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBN4798173.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGTTDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            8878..10536
                     /locus_tag="L2X49_003136"
     CDS             8878..10536
                     /locus_tag="L2X49_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBN4798174.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10533..11489)
                     /locus_tag="L2X49_003137"
     CDS             complement(10533..11489)
                     /locus_tag="L2X49_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBN4798175.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            11640..12755
                     /gene="macA"
                     /locus_tag="L2X49_003138"
     CDS             11640..12755
                     /gene="macA"
                     /locus_tag="L2X49_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBN4798176.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQELDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            12752..14698
                     /gene="macB"
                     /locus_tag="L2X49_003139"
     CDS             12752..14698
                     /gene="macB"
                     /locus_tag="L2X49_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBN4798177.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDYFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14771..14995)
                     /gene="cspD"
                     /locus_tag="L2X49_003140"
     CDS             complement(14771..14995)
                     /gene="cspD"
                     /locus_tag="L2X49_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBN4798178.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            15318..15638
                     /gene="clpS"
                     /locus_tag="L2X49_003141"
     CDS             15318..15638
                     /gene="clpS"
                     /locus_tag="L2X49_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBN4798179.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            15669..17945
                     /gene="clpA"
                     /locus_tag="L2X49_003142"
     CDS             15669..17945
                     /gene="clpA"
                     /locus_tag="L2X49_003142"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBN4798180.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(18289..18376)
                     /locus_tag="L2X49_003143"
     tRNA            complement(18289..18376)
                     /locus_tag="L2X49_003143"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18340..18342),aa:Ser,seq:gga)
     gene            complement(18630..>18720)
                     /locus_tag="L2X49_003144"
     CDS             complement(18630..>18720)
                     /locus_tag="L2X49_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBN4798181.1"
                     /translation="YIRILTGDKVTVELTPYDLSKGRIVFRSR"
BASE COUNT         4616 a         4700 c         5042 g         4362 t
ORIGIN      
        1 gcgcttaccg ctcaccgcca taaaatgttc aaactgttgc tgataactga gcacctctgg
       61 tccggcggct tcgaagatgc ggtgttcgct ggccggatga tctaacaacg ccaccagata
      121 gtgcagcaag ttttccagcg cgatgggcgt ggtgcgtgaa cgtacccagc gtggcggcgt
      181 taacactggc aggttgtaga ccatatcgcg catgacttcg aacgccgctg aacctgcgcc
      241 aacgataatt ccggcccgaa gttcggtcac aggtacattc gcttcacgaa gaatgtccgc
      301 cgtagcctga cgagcacgca gatgatccga ctgctcatgt ggcggggcct gcaacgaact
      361 gagaaagatt aattgcttaa ctggtacttc acgtagcgca tcgcggacgt tgagagccac
      421 ctggcgctcc tgagcgataa aatcgccgcc ttcgcccatg ctgtgcacca gaaaatagac
      481 ggtatcgata tcctgcaaca gggccggaag gttatccggc cagctgagat cgactttatg
      541 gcaactgacg tttgccagtt gcagctttgc aagcctgtcg acatgacgtg ccgccgccag
      601 gatctgatgc ccttgctggc tgagtgtgcg caccagatgc tgaccaatgt agccactggc
      661 accgagaact aaaatgcgtt gcggcacgtc tctctcctta acgcgccagg aatgcgcgcc
      721 agtgggcggc gacttccgcc agttgttcgc gcgagacgtc aagatgcgtc accaggcgga
      781 caatcggcga ggcgttaatc agcacgtttc tcgctttcat gtattcgcct aacgcggcag
      841 cattttcttc cccgacgcga acaaacagca tattagtgtc ctgacgcatc acatccgcgc
      901 ctgcttcacg cagttgctcc gccatccagg cggcgttgtc gtgatcttcc tgcaaccgtg
      961 cgacgttatt tttcagggca tacatcccgg cggcagccag aatcccggac tgacgcatcc
     1021 cgccaccggc cattttccgc cagcggatgg cacgtttaat gtaatcacga ttaccgacga
     1081 gtaatgaacc gactggcgtc ccaagatctt tcgacaggca aatggtgaac gaatcacaat
     1141 attgcgtgat ctctttcagt tcgcagccgt aagccaccac ggcattaaag atgcgcgcac
     1201 cgtcaacatg cagcgccaga ttgcgctcgc gggtaaattc ccatgcttct ttcaggtatt
     1261 cacgcggcag cactttgccg ttgtgggtgt tttccagact gagtaatttg gtgcgggcga
     1321 aatggatatc gtcgggtttg attttcatcg ccactttatc cagcggtagc gtgccgtcgg
     1381 cagccgcgtc gatgggttgc ggctgaatac tgcccagcac cgctgcgcca ccggcttcaa
     1441 acagatagtt atgcgcggcc tgaccgacaa tatactcttc gccacgttcg cagtgactga
     1501 gcagagcgac caggttggcc tgagtgccgg taggcagaaa aatggcggct tctttaccgg
     1561 aaagctctgc tgcatagtcc tgcagagcat taacggtagg gtcgtctccg taaacgtcgt
     1621 ccccaaccgg ggcggccatc atcgcttcga gcatggcgcg gctcggtcgg gtaacggtat
     1681 cactgcgtaa atcaatcatg gcatgtcctt attatgacgg gaaatgccac cctttttacc
     1741 ttagccagtt cgttttcgcc agttcgatca cttcatcgcc gcgtccgctg ataattgcgc
     1801 gcaacatata caggctaaaa cctttggcct gttcgagttt gatctgcggt ggaatggcta
     1861 actcttcttt ggcgaccacc acatccacca acaccggacc gtcgatagag aaggcgcgtt
     1921 gcagggcttc atcaacttca gacgcttttt ctacacggat acccgtaatg ccgcacgctt
     1981 cggcaatgcg ggcaaagttt gtgtcgtgta gttcggtgcc gtcagtcaaa tagccaccag
     2041 ctttcatctc catcgccaca aagcccagca cgctgttgtt aaagacgaca attttcactg
     2101 gcagtttcat ctgcactact gagaggaaat cgcccatcaa catgctaaaa ccgccatcgc
     2161 cgcacatggc gaccacctga cgttctggct ctgtcgcctg cgcacccagc gcctgcggca
     2221 tggcgttagc catcgaaccg tggttaaacg aacctaacag gcgacgcttg ccgttcattt
     2281 ttagataacg tgccgcccac accgttggcg taccaacgtc acaggtgaaa atagcgtcat
     2341 cggcggcaaa atgactaatt tgctgcgcca gatattgcgg gtgaatggct ttctcgctcg
     2401 gtttagctaa atcgtccagc cctttgcggg cgtcgcggta atcttccagc gctttatcca
     2461 gaaacttgcg atcggctttt tcttccacca atggaagcaa tgcacgcaga gtcgacttga
     2521 tatcgccgac cagtgccata tccaccttgc tgtgagcgcc gatgctggct gggttgatat
     2581 caatctgaat gattttggca tcggtcgggt agaaggcgcg gtagggaaat tgcgtgccga
     2641 gtagcactaa cgtgtcggcg ttcatcatgg tatggaaacc tgacgagaag ccgattaacc
     2701 cggtcattcc aacatcatac ggattatcgt attcgacatg ttctttaccg cgcagggcat
     2761 gaacaatagg cgctttaatt ttcccggcaa actcaactaa ctctttatgc gcccccgcgc
     2821 agccgctgcc acacatcagg gcgatattgc tggaataacg cagcagttgc gccagtttgc
     2881 gtaactcttc ttcttccggc gtcacgactg gttgtggcgc atgataccag tgcgtggttg
     2941 ccccttctgg cgcaggtttt aacgccacgt cgcctggtaa cacgacaacc gaaacgccac
     3001 ggttaagcac cgctttgcgc atggcaatcg ccagtacttg tgggatctgc tccgggctgg
     3061 aaaccagctc gcaatagtga ctacattcgc ggaatagctc ttgtgggtgg gtttcctgga
     3121 aatagccgct gccaatttcg ctggagggaa tatgagcggc aatcgccagt accggaacgt
     3181 gattgcggtg gcaatcgaac aggccgttga ttaagtgcag gttgccgggg ccgcacgatc
     3241 cggcgcagac cgccagttct ccgctaagtt gtgcttcagc gccagcggca aaggccgcca
     3301 cttcttcgtg gcgggtggac atccactcga tggtgcccat gcgattaaga ctgtcactaa
     3361 gaccgttcag agagtcgcca gtgactcccc agatgcgttt cacccctgcc gattcgagtg
     3421 ttttggcgat ataagctgca accgtttgtt tcatggttct ccatctcctg aatgtgataa
     3481 cggtaacaag tttagttcat ctgacggagg gggaagggat gggagagaaa ggaggcacta
     3541 acggttaaat agcccgatga aaggaatatc atcgggtata aggcgattat gcgagaacca
     3601 aatccccctg cggatggcag gagcaggcca gtacgtaacc ttcagcgatt tcggcgtcgg
     3661 tcagcgtcat tgtactgctc accgtatatt caccggaaac cacttttgtc ttacagcagc
     3721 cgcaaacacc cgcacggcag gcagcgacaa ccggaacgtt attgctttcc agcgcctcca
     3781 gtagcgtggt gccaaccggg gcgtaaaatt ctcgtgccgg ttgcagtttg gtgaatttca
     3841 gaccgctggt cgccgcttct gctactgggg tgaagaattt ctctttaaag aaacgcgtca
     3901 cgccgagcgc tttcacttcc tgctctaccc aatccatata cggagccggg ccgcaggtca
     3961 tcacggtacg tgaagctaag tcaggtacac ctgccagcag ttcgcgagtg agacgaccag
     4021 cgataaagcc ttcggtaacg ttattttctg ccaccagcgt taccggatag ttacgccact
     4081 catcggcgaa aataacatcc tgcggcgtac gcacgttgta gatcacccgc acatcggcct
     4141 gtggacggtt cttcgcaagc cagcgacgca tcgacataat cggcgtgacg ccgcagcctg
     4201 ccgccagcaa caggaattta tcttctgctt tatcgtcgca ggtaaattcc cccatcgcgt
     4261 ccgaaagcca gagataatca ccgcgtttta catcgcgcgt cagccactgg gagccgacac
     4321 cgtcatcaat ccgccgcacg gtcagggtga tatattcact cacgcctggc gtggaggaaa
     4381 tggtgtaagc acgcagcgtt tccgctgagt ttcgcacgct gaccagtgca tattgcccgg
     4441 cgcgatatgg gtagtaatcg tggcaaatca gggaaatcgt ccacacatcc ggcgtttctt
     4501 gcgtaatgtg atgaaattgc atccgccacg ggcattgatt cgttggcatc gtcatcgaca
     4561 aactccttac gcgctcaaca gttgcttcat gtcttcttca acagtggtga tagaacgcag
     4621 gccaaatttc tcgttcagca ccgccagcag gtctggtgtc aggaaaccag gtgcagtcgg
     4681 gccggtgacg atatttttca cgcccagaga aagcagcgtc agcagaatga cgatcgcttt
     4741 ctgttcaaac caggagagca ccagcgacag cggcagatcg ttgacaccgc agcccagttt
     4801 ctctgccaga gtgacagcca gaataatcgc tgagtaagca tcgttacatt gacctgcatc
     4861 taccagacgc ggcagacctt cgatatcgcc aaactcaagt ttgttaaagc gatatttacc
     4921 acaggcgagg gtcaggatca ggcagtcatc cggcacgctg gtggcgaaat cggtgaagta
     4981 gtggcgctcg ccgcgtgcgc cgtcacagcc accaagcagg aagatatgac gcagtttttc
     5041 acggctcacc agatcaatca gcgtatccgc tgcgccaagc agcgtctggc ggccaaagcc
     5101 cacggtgatc aggtgcggaa tttcgctgta cgggaagccc gccatttgtt gcgcctgggc
     5161 gatgaccgca gaaaaatctt cgccatccag atgacgcaca cccggccagc caacgatgct
     5221 gcgggtccag atacgatcgt cataagcgcc tacggttggg tcgatgatgc agttcgaggt
     5281 catcacgatg gggccaggga aacgagcgaa ctccacttgc tgattctgcc agccgctgcc
     5341 gtagttaccg accagatgct tgaatttacg cagctccggg tagccatgcg caggcagcat
     5401 ttcgccgtgg gtgtaaacat taacgcccgt gccttcggtc tgttccagca ggttgtagag
     5461 atctttgaga tcgtgaccgg aaatcaggat gcatttaccc gccgtcgctt tgacgttgac
     5521 ctgggttggc gtcgggtgac cgtatttacc ggtttcgcct gcatccagaa tgctcatcac
     5581 tttgaagttc atctggccga tttccattga acactcaaga agcgcgttca tatcggcagg
     5641 ccaggtcccc agccacgcca tgattttatg atactgggca taaatatcgt tgtcgtattg
     5701 accgagaaca tgcgcgtgtt ccatataggc cgctgcacct ttcaggccat acaggcacag
     5761 cagacgcagg ccgagaatgt tttcgccaat cgccgcttta tctttgttag gggtaaattc
     5821 tgctgcctga cgttgcagct cgccgagatc gtcgctcacc agttgcaggt cagccatcgg
     5881 gttatcgacg cgcgcgttgg catctacagc caggcattgc gctttcagcg cctcgcgcag
     5941 ggcaatcgct tcacgagcgt agccgacaat acgcggagaa tcgaagttaa cgttggtcag
     6001 ggttgagaaa aaggcacgtg gcgcgaagct gtcaacatcg tggttgatga tgccgtattc
     6061 acgcgctttt accgcccagg cagaaagccc ttgcagcgcc gcgatgagta aatcctgaag
     6121 gtcagaagtt tccgccgttt taccacacat cccctgcgcg tatgagcagc cgtttcctgc
     6181 cggagtacgg atagtttgtt cacattgcac acaaaacatg atcacacctt ttaaagttat
     6241 atttaatata catgtttaag gttaagacgc ttaacgcggg gataaaaggg atttttcatg
     6301 caactttaag ggagattgat ttagcgcaat tttggcggca gggatctacc gccagagagg
     6361 tattacgcag agaaaaaggc gatgaggatc ggcactaaca ggctaagaat aaaaccgtga
     6421 acaattgccg ccgggaccat atccagcccg ccagtacgtt gaagaacggg cagggtgaaa
     6481 tccattgatg tggcaccgca taagcccagt gcagtagagc ggctgcggcg aatcagccca
     6541 gggatcaaca taatagcaat cagttcacgg gccagatcat taaaaaacgc cgcgctcccg
     6601 attaccggac caaaagattc ggtcaataaa ataccggaaa gagaatacca gccgaaaccg
     6661 gaggccattg ccagcgcggt attgatgggg agatcaagaa taaaggcgtt aattaaacca
     6721 ccaattaatg aactgacaac caccaccacg gcgacaatca ttccccggcg attaaggaca
     6781 atctgcttta aggtcatgcc attattgcgc aactgaatac caacgaggaa aagtagcaaa
     6841 attaacgtgt attcactggc ttcggtcgcg tgttgtaaga aagccagtcc actcagacca
     6901 atggcaaaac caatcactac tacgccgcac agttttagcg actccagcgc catcgcaata
     6961 cgcgacggga gtttttcttg ctgatggtgg ttgcgccacg gcaggcctcg ctccagccac
     7021 atcagggcgg caatattaca cagtaaaata acggtaatac tgacggcaga ataatgcaga
     7081 atcgccaaca ggttactggc gaggttatcg agaaacgcca gactgatacc cataaaaaag
     7141 agaataaggt aaaccatcca gcttaatagc tgattaataa cttttaacgc agcttgttgg
     7201 cgaagcggaa tgaggtaacc cacaatcagg ggaaccagaa tgattaacag cccagaaaac
     7261 atgaaaaccc agtccttgca aagatgaagt cgaaatgcgc gatgacacac tactgaaagc
     7321 ggaaggacga gtaaagttgc aattaaaagg aaatgttatg cataaggagc agtagagtat
     7381 tcgttttcat ttaaagatat tcttgcgctt taattacaaa ctgcaccgat gttggtggcg
     7441 tcaaaatcgc cgaggcgttc cctgaaggcc ggggcagccc acatggatgt gggctgaggg
     7501 cgcgttttac agggatgtta cctcgcgccc gacccggtag ccgtaaggga taagtcgagg
     7561 gcaccgcgca gcggcgattt tgttcgccag agcccggggg tgcagggggc ggcggcgatt
     7621 ggccgccccc tgcgcgctct ttgcgccagt ggcaatatgt ggcttagctc atgaaaggag
     7681 cgcaacaaga tgatgaatca acatataaca acatcttaaa aaaaggcctg acattacgcc
     7741 aggccttctg cgttaattaa tcacgctttt ccagcagggt ccggtaaatc agaccaccga
     7801 taatgccgcc gacaattggc accacccaga agaaccacag ttgttctaat gcccagccgc
     7861 cctggaagat agcaaccgcg gtgctgcgcg ccgggttaac agaagtgtta gtcaccggaa
     7921 tactaattaa gtgaatcagg gttaaggcca gaccaatagc gatcggcgca aaacctgccg
     7981 gcgcgaattt gtcggttgtg ccgtggatca ccaacaggaa acctgcactc aataccagtt
     8041 caactaccag cgcggaaagc atggaataac cgcctggtga atgctcgcca taaccgttag
     8101 aagcaaaacc gctggctgcc gcgtcaaaac ccgttttacc actggcaatt aaatacagca
     8161 gcgccgctgc aacaataccg ccgacaacct gggcaattac gtagccaacg acttcttttg
     8221 ccggaaaacg tccgccagcc cataaaccaa tagtgaccgc cgggttaaaa tgaccaccag
     8281 aaatatgacc aacagcaaag gccatcgtca gaacggtcag accgaacgcc aacgccacgc
     8341 cggcaaaacc aatgcctaat tccgggaagc ctgcggccag tacagcacta ccacagccac
     8401 caaaaacaag ccagaaagta ccaaaacatt cagctgctaa ttttctgaac atatccacct
     8461 caattaaaaa ttgaccctgt gaaaaatatg gtcgttttat agggtcgtcg taaaaaagtg
     8521 acgacggaaa taatgcgcgg ctattttaaa aacgaaggcg agtcattcac cagataaata
     8581 aatccagtaa atttgattta gggcaacagc gggttgcccc atatagtcat ttgtctgatt
     8641 gacagtgtag tgcaagcaaa agatttaatc ctttaggcgt aataaaaaat aatttatcat
     8701 gctaattatt tgattttgtt gtttttgcag acttatcagc aagagggagt ataacgcgat
     8761 tattcgctca tttttcagac atttgccatg cttaaatgtg atgtcatcac gtattagcaa
     8821 ggcctttccc gttatactgc cagcgtaaag gataagtcac atatttctgg aggggatatg
     8881 attcttgagc gcgttgaaat tgtgggtttt cgcggtatca accgtttgtc gttgatgctc
     8941 gaacaaaaca acgtcctgat tggggagaac gcgtggggta aatccagctt gctggacgcc
     9001 ttaaccctgc tgctatcgcc agaatcagat ctctaccatt ttgagcgcga cgatttctgg
     9061 ttcccgccgg gagatatcaa cgggcgagaa catcatctgc atattatttt gaccttccgc
     9121 gaatcgctgc caggccgaca tcgggttcgc cgttatcggc cgctggaagc gtgctggacg
     9181 ccatgcaccg atggctatca ccgtattttt tatcgtctgg aaggggagag tgcggaagac
     9241 ggcagcgtga tgacactgcg cagttttctc gataaagacg gacatccgat tgatgtcgag
     9301 gatattaacg atcaggcacg ccatctggtg cgtttaatgc cggtgctgcg cttgcgtgat
     9361 gcccgtttta tgcgccgtat tcgtaacggc acggtgccaa atgttcccaa tgtggaagtc
     9421 accgcgcgcc agctcgattt cctcgcccgt gagttgtcct cacatccgca aaatctctct
     9481 gatggacaga ttcgtcaggg actttccgca atggtgcagt tgcttgagca ttatttctct
     9541 gagcaggggg ccggacaggc gcgatatcgt ttaatgcggc ggcgggccag caatgagcaa
     9601 cgaagctggc gctatctgga catcatcaac cggatgattg accgacctgg agggcgctcg
     9661 tatcgggtta ttttgctcgg cctgtttgct actttattgc aggcaaaagg cacattgaga
     9721 ctggataaag acgcgcgtcc attgttgctg atcgaagatc cagaaacccg tttacacccc
     9781 attatgcttt cagttgcctg gcatctgttg aatcttctgc cattgcagcg cattgccacc
     9841 accaactcgg gtgagttgct ttcgttaacg ccggtagagc atgtttgccg actggtacgt
     9901 gagtcctcgc gcgttgccgc ctggcgtctg gggccgagtg gcttgagtac cgaagatagc
     9961 cgacgcatat cctttcacat tcgttttaac cgtccgtcat cgctgtttgc tcgctgctgg
    10021 ttgctggtgg aaggggaaac ggaaacctgg gttatcaatg aactggcgcg ccagtgtgga
    10081 caccatttcg atgccgaagg gatcaaggtc attgagtttg cccagtccgg gctaaagcca
    10141 ctggttaaat ttgcccgccg aatggggatt gaatggcatg tactggtcga tggcgatgaa
    10201 gcagggaaga aatatgccgc tacggtacgc agcctgttga ataacgatcg ggaagccgaa
    10261 cgagaacatt taacggcgtt accggcgctg gatatggaac attttatgta tcgccaggga
    10321 ttttccgatg tgttccaccg cgtggcgcaa atcccggaaa atgtaccgat gaatctgcgc
    10381 aaaattatct cgaaagcgat ccatcgctct tccaaacccg atcttgccat tgaagtggca
    10441 atggaggccg gacgtcgtgg tgtggactcc gtaccgacgc tgctgaaaaa aatgttctca
    10501 cgcgtgctgt ggctggcgcg cggtcgcgcg gattaaccgc gaaacatcgt ggccatttgt
    10561 ggctgaatag cgtcgagcat ctcatagcgc cgacggtatt cagcccgttt tttactggcg
    10621 atttcggcaa tctcttttcg tgctatctgt gctggaaggc ggtaatggcg ttcagcatca
    10681 catacgccgc caaccgattc ccagaaagcg ttgtaatcgg catggatctt gccttcttta
    10741 tcacgataac gcaggctgcg gtaaatatgc gtttcattgc tgacggcaat aatctgctct
    10801 acctgcaaac gttgggcaaa cagacaggcc gcttccatca cgaggcgttt gggaaatagc
    10861 ccgtggcagg ctttcgtcgc attctggatt tcctgatgtg gaatttccca ttttgcgcct
    10921 tgcagtccgc caataaacat cgttcttttc ccctgatatt cacacagggt aaacgtgatc
    10981 tctgccagag gaataccttc gctgttgcga aacaggattg tgctgtcacc ttctttatcc
    11041 attgagatca tcatggtcag ctcaagcgtg aactgctcgc cgtttttgcc ttccagttta
    11101 gccagttgca gcccgggggt attcaaatat aagctgaatt cttccgccga catacatccg
    11161 cggagtaacg cataatggta acgtaacgcc tccagcaatt gcttacggct aagattcgcc
    11221 gcaaggtaag ggcgatgcag acgcacaggc agtcgcggtt ggcgcgttaa caacacattg
    11281 agattaggcc agtgggaaag ttcgttcatc cactcaacgc ttaaacgcgg cataatcaac
    11341 gagcgcagca aaaatttctg gcgaaaacta cggcgatgcc agaatttacc cggccgacac
    11401 tgtccacgtg ccagactaag aaaaagtgac aggctgctga gagattcaga tggcgtaaag
    11461 gtccgttcag ttagctgcga catattcatg aaatcaatgg ttatacatga cgtcgatttc
    11521 accattgcgt atcttaacca aacatcaata gtgtgattac taacgtaaat tttagggttt
    11581 tgttgatatt tcgttgaagt taatgacccg gattggcata tggagtattc agaatattta
    11641 tgaaaaagcg gaaaaccgtg aagaagcgtt acgttattgc gctggtgata gtcatcgccg
    11701 gactgattac gttatggaga attcttaacg cacccgtgcc gacttatcag acactgattg
    11761 tgcgccccgg tgatttacag caaagcgtgc tggcgaccgg aaagctggac gcgctgcgta
    11821 aggttgacgt gggcgcgcag gtcagcggtc agttgaaaac tctgtcggtg gcgattggcg
    11881 ataaagtaaa aaaagaccag cttttagggg ttattgatcc tgaacaggct gaaaaccaga
    11941 ttaaggaggt cgaagcaacg ctgatggagc tacgtgcgca gcggcagcag gcggaagcgg
    12001 agctgaaact ggcgcgggtg acgtattccc gtcagcaacg tctggcacaa acgcaggcgg
    12061 tttcacagca ggagctcgac accgccgcga cggagatggc tgtgaaacag gcgcaaattg
    12121 gcaccattga cgcgcaaatc aagcgcaatc aggcctctct cgatacggct aaaaccaatc
    12181 tcgattacac tcgcatcgtt gccccgatgg ccggggaagt cacgcaaatc accactctgc
    12241 aaggccagac ggtgattgcc gcacaacaag caccgaacat tctgacgctg gcagatatga
    12301 gcaccatgct ggtaaaagcg caggtttctg aagcggatgt aatccacctg aagccggggc
    12361 aaaaagcctg gtttacggta cttggcgatc cactgacgcg ctacgagggg caaatcaagg
    12421 atgtactacc gacgccggaa aaggttaacg acgctatttt ctattacgcc cgttttgaag
    12481 tccccaaccc caatggtttg ctgcggctgg atatgactgc gcaagtgcat attcagctca
    12541 ccgatgtgaa aaatgtgctg acgatccctc tgtcggcgtt aggcgatccg gttggcgata
    12601 atcgttataa agtcaaattg ttgcgtaatg gtgaaacacg cgagcgtgaa gtgacgattg
    12661 gcgcacgtaa cgataccgat gttgagattg tcaaagggct tgaagcgggc gatgaagtgg
    12721 tgattggtga ggccaaacca ggagctgcac aatgacgcct ttgctcgaat taaaggatat
    12781 tcgtcgcagc tatcctgccg gtgatgagca ggttgaggtg ctgaagggca tcaccctcga
    12841 tatttatgcc ggtgagatgg tagcgattgt tggcgcttca ggttccggta aatcgaccct
    12901 gatgaatatt ctcggctgtc tggataaggc caccagcggc acctatcgcg tcgccggtca
    12961 ggatgttgcc acactggacg ccgatgcgct ggcgcaactg cgccgcgagc atttcggctt
    13021 tattttccag cgttaccatt tactttcgca tttaaccgcc gagcagaacg ttgaagtacc
    13081 cgccgtctat gctggtcttg agcggaaaca gcgactgctt cgtgcccagg agttgctgca
    13141 acggctgggg ctggaagacc gtacagagta ttatccggca cagctttcgg gtggtcagca
    13201 acagcgcgtc agcatcgcgc gggcattgat gaacggtggt caggtaattc ttgccgatga
    13261 accaaccggc gcactggaca gccattctgg cgaagaggtg atggcgatcc tgcatcagct
    13321 gcgcgatcgt gggcatacgg tgattatcgt cacccacgat ccgcaggtcg ctgctcaggc
    13381 cgagcgggtg atcgaaattc gcgacggcga aattgtgcgc aatcctcccg ccattgaaaa
    13441 agtgaatgtt gctggcggga cggagcctgt tgtcaacacg gtgtctggct ggcggcagtt
    13501 tgtcagcggt tttaacgagg cgctgacgat ggcatggcgg gcgctggcag cgaataaaat
    13561 gcgtacttta ctgaccatgc tggggattat tatcggtatt gcgtcggtgg tttccattgt
    13621 cgtggtgggt gacgccgcca aacaaatggt gctggcggat attcgttcta ttggtacgaa
    13681 tactattgat gtctatcccg ggaaagattt tggcgatgac gatccgcaat atcagcaggc
    13741 gctgaagtac gacgacttaa tcgccatcca aaaacaaccg tgggtcgcct cagccacacc
    13801 tgccgtctcg caaaacctgc gcctgcgtta taacaatgtt gatgttgctg ccagtgccaa
    13861 tggcgtgagc ggcgattatt ttaatgtcta tggcatgacc ttcagtgaag gaaacacctt
    13921 taatcaggag cagctgaacg gtcgtgcgca ggtcgtggtt ctcgacagta atactcgccg
    13981 ccagcttttc ccccataaag cagatgtggt tggcgaggtg attctggtcg gcaatatgcc
    14041 cgccagagtc attggtgtgg cggaagaaaa acagtcgatg tttggtagca gtaaagtgct
    14101 gcgtgtctgg ctaccttaca gcacgatgtc cgggcgagtt atgggccagt cgtggctgaa
    14161 ctccattact gtcagggtaa aagaaggatt tgacagcgcc gaggcggaac agcaactcac
    14221 gcgtttactt tcactgcgcc acggaaagaa ggattacttt acctggaaca tggacggcgt
    14281 cttgaaaact gttgaaaaga ccacacgtac tttacaactg tttctgacgc tggtggcggt
    14341 gatttcgctg gtggtgggcg gtattggtgt aatgaatatt atgctggtgt cagtgaccga
    14401 gcggacgcgg gaaattggca ttcgcatggc tgtaggtgcg cgagcaagcg atgttttgca
    14461 acagttcctg atcgaagccg tactggtttg cctggtcggt ggcgcgttgg gaataacact
    14521 gtcactgtta attgctttca ccttgcagct tttcttaccc ggctgggaga ttggtttttc
    14581 accgttggca ctgctgctgg cgtttctctg ctcgacggtc accgggattt tatttggatg
    14641 gttacccgca cgaaatgcgg cacgactgga tccagtagat gctctggcac gagagtaatt
    14701 tttgagataa aaatgccagc cgatcgggct ggcattttgc ctttaggatg tacacaatga
    14761 gacagaagag ctatgcgact gccgcttcta cttcgacggg cacaataaca ctggcgtgat
    14821 tgccttttgg cccctggtgg acatcaaact gaacggattg tccagctttt agcgttctgt
    14881 aaccatccat ctgaatggtg gaataatgag cgaaaatatc ttcgccgccg ccttcagggc
    14941 agatgaaacc aaaccctttg gcattgttga accacttaac agtacccttt tccatgcttc
    15001 gacatccttc gcaaatctta tacaagtaag atggaataaa ccggggtcag agagggggct
    15061 gttcaaaacc tcgccaactc tagaaataca atttagagaa ttagggcgag ccgtcaagcg
    15121 tttgacaggg gccagggggc aggtatgaat caaaaatttg aagcagttaa cgctattgac
    15181 aggaatgtga cagatgtcgc tgatgccaac gatagatgat agttatctat catgtggagt
    15241 agattggtca ggcaaataag ctcttgtcag cggcagggcg ttctgccgat aaccgtaacc
    15301 gaagatgata actgacaatg ggtaaaacga acgactggct ggactttgat caactggcgg
    15361 aagaaaaagt tcgcgacgcg ctaaaaccgc catctatgta taaagtgata ttagtcaatg
    15421 atgattacac tccgatggag tttgttattg acgtgttaca aaaattcttt tcttatgatg
    15481 tagaacgtgc aacgcaattg atgctcgctg ttcactacca ggggaaggcc atttgcggag
    15541 tctttaccgc cgaggttgca gaaaccaaag tggcgatggt gaacaagtac gcgagggaga
    15601 atgagcatcc attgctgtgt acgctagaaa aagcctgaat gcaggcataa aaattggggg
    15661 aggtgcctat gctcaatcaa gaactggaac tcagtttaaa tatggctttc gccagagcgc
    15721 gcgagcaccg tcatgagttt atgaccgtcg agcacttgtt actggcgctg ctcagtaacc
    15781 catctgcccg ggaggcgctg gaagcgtgtt ctgtggattt ggttgcgctc cgtcaggaac
    15841 tggaagcctt tattgaacaa accacacccg ttctgcctgc cagtgaagag gagcgcgaca
    15901 cacagccgac gctgagtttt cagcgtgtac tgcaacgtgc ggtcttccat gtccagtcct
    15961 ccggtcgcaa tgaggtaacc ggtgcaaacg ttctggtcgc tatctttagc gaacaggagt
    16021 cgcaggcggc atatctgttg cgtaaacatg aagtcagccg tctcgatgtg gtgaacttta
    16081 tctctcatgg cacgcgtaaa gacgagccga cacagtcttc tgatcctggc agccagccaa
    16141 acagcgaaga acaagctggt ggggaggaac gtatggagaa tttcacgacg aacctgaatc
    16201 agcttgcgcg cgtgggcgga atcgacccac tgattggtcg tgagaaggag ctggagcgtg
    16261 ctattcaggt tctctgccgt cgccgtaaaa acaacccgct gctggtgggg gaatctggtg
    16321 tcggtaaaac cgcgattgcg gaaggtcttg cctggcgaat tgttcagggc gatgtgccgg
    16381 aagtgatggc tgactgtacg atttactctc tcgatatcgg ttctctgtta gcgggcacta
    16441 aatatcgcgg cgactttgaa aaacgtttta aagcgttgct caagcagctg gagcaggaca
    16501 ctaacagcat cctgtttatt gatgagatcc acaccattat cggtgcgggt gcagcgtctg
    16561 gtggccaggt cgatgcggct aacctgatca aaccgttgct ctccagcggt aaaattcgcg
    16621 taattggttc gacaacctat caggagttca gcaacatttt cgagaaagac cgtgctctgg
    16681 cgcgtcgctt ccagaaaatt gatattactg aaccgtcgat cgaagaaact gttcaaatca
    16741 tcaatggcct gaaaccgaag tatgaagcgc accacgacgt gcgttatacc gcaaaagcgg
    16801 tgcgtgcagc ggtagagctg gcggtgaaat acattaacga tcgtcatctg ccggataaag
    16861 ccattgacgt tatcgacgaa gcgggcgctc gcgcacgcct gatgccggta agcaaacgca
    16921 agaaaaccgt taatgtggcg gatattgagt ccgtggtggc ccgtattgcg cgcattccag
    16981 agaagagtgt ttctcagagt gaccgcgata ccctgaaaaa cctcggcgat cgcctgaaaa
    17041 tgctggtctt cggtcaggat aaagccattg aggcgctgac tgaagccatt aagatggcgc
    17101 gtgcaggttt aggtcacgaa cataaaccgg ttggttcgtt cctgtttgcc ggccctaccg
    17161 gggtcgggaa aacagaggtg acggtacagc tttcgaaagc gttgggcatt gagctgctgc
    17221 gctttgatat gtccgagtat atggaacgcc ataccgtcag ccgtctgatt ggtgcgcctc
    17281 cgggatacgt tggttttgat cagggcggtt tgctgactga tgcggtcatc aagcatccac
    17341 atgcggtgct gttgctggac gaaatcgaga aagcgcatcc ggacgtgttc aatattctgt
    17401 tgcaggtgat ggacaacggt acgctgaccg ataacaacgg acgcaaagcg gacttccgta
    17461 acgtggtgct ggtgatgacc accaacgccg gggtacgtga aactgagcgt aaatccattg
    17521 gtcttatcca ccaggataac agcaccgatg cgatggagga gatcaagaag atctttacac
    17581 cggaattccg taaccgtctc gacaacatta tctggtttga tcatctgtca accgacgtga
    17641 tccatcaggt ggtggataaa ttcatcgtcg agttgcaggt tcagctggat cagaaaggtg
    17701 tttctctgga agtgagccag gaagcgcgta actggctggc cgagaaaggt tacgaccggg
    17761 caatgggcgc acgtccgatg gcgcgtgtca tccaggacaa cctgaaaaaa ccgctcgcca
    17821 acgaactgct gtttggttcg ctggtggacg gcggtcaggt caccgtcgcg ctggataaag
    17881 agaaaaatga gctgacttac ggattccaga gtgcacaaaa gcacaaggcg gaagcagcgc
    17941 attaatctga ttgttaggta ggttggtcaa gtccgtaatc tcgaaagagg ttacggactt
    18001 tttgtttatg tggtggagga ggttcagacc ctttttttaa tgatgatggt aagttgttga
    18061 taattagtgc tgcgggaagg taaggataaa aaagggtgct gcaggagaat gggatggttt
    18121 tgctttatta acaacgggct aaacgtgtag tatttgagtt cactgccgta caggcagctt
    18181 agaaatcgac ggggtgcggt aaaacctttg cgaacgcgtt cactgccgta cagacagata
    18241 aaatgcgaaa aaaaagctcg cactttcgta cgagctcttc tttaaatatg gcggtgaggg
    18301 ggggattcga acccccgata cgttgccgta tacacacttt ccaggcgtgc tccttcagcc
    18361 actcggacac ctcaccaaat tgttttgcta ccaaacctca tgggtggcaa cggggcgcta
    18421 ctatagggag ttggagtaaa acggtcaaga agaattttaa tgataattat tgtttgctca
    18481 tactgtaaac aagttgtgca gtatatctac atcgagacaa gttacggact tatacttcca
    18541 aagtacttca tacatatcac aaaataaaaa ggccggttaa accgaccttt tactcgttct
    18601 ttctcttcgc ccatcaggcg gtaaaacaat cagcgactac ggaagacaat gcggcctttg
    18661 ctcaggtcgt acggggtcag ttcaacagtc actttgtcgc ccgtcaggat gcggatgtag
//
LOCUS       DAFNBJ010000097        18412 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000097 DAFNBJ010000000
VERSION     DAFNBJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.097"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            4..1302
                     /gene="dadA"
                     /locus_tag="L2X49_003145"
     CDS             4..1302
                     /gene="dadA"
                     /locus_tag="L2X49_003145"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBN4798182.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            1312..2382
                     /gene="dadX"
                     /locus_tag="L2X49_003146"
     CDS             1312..2382
                     /gene="dadX"
                     /locus_tag="L2X49_003146"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBN4798183.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(2768..4504)
                     /locus_tag="L2X49_003147"
     CDS             complement(2768..4504)
                     /locus_tag="L2X49_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBN4798184.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(4599..5513)
                     /gene="ldcA"
                     /locus_tag="L2X49_003148"
     CDS             complement(4599..5513)
                     /gene="ldcA"
                     /locus_tag="L2X49_003148"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBN4798185.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            5613..6224
                     /gene="emtA"
                     /locus_tag="L2X49_003149"
     CDS             5613..6224
                     /gene="emtA"
                     /locus_tag="L2X49_003149"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBN4798186.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(6226..6960)
                     /locus_tag="L2X49_003150"
     CDS             complement(6226..6960)
                     /locus_tag="L2X49_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBN4798187.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            7161..7415
                     /locus_tag="L2X49_003151"
     CDS             7161..7415
                     /locus_tag="L2X49_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBN4798188.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            7593..8033
                     /locus_tag="L2X49_003152"
     CDS             7593..8033
                     /locus_tag="L2X49_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798189.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(8112..9809)
                     /gene="treA"
                     /locus_tag="L2X49_003153"
     CDS             complement(8112..9809)
                     /gene="treA"
                     /locus_tag="L2X49_003153"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBN4798190.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(10129..11547)
                     /gene="dhaM"
                     /locus_tag="L2X49_003154"
     CDS             complement(10129..11547)
                     /gene="dhaM"
                     /locus_tag="L2X49_003154"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBN4798191.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
     gene            complement(11558..12190)
                     /gene="dhaL"
                     /locus_tag="L2X49_003155"
     CDS             complement(11558..12190)
                     /gene="dhaL"
                     /locus_tag="L2X49_003155"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBN4798192.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(12201..13271)
                     /gene="dhaK"
                     /locus_tag="L2X49_003156"
     CDS             complement(12201..13271)
                     /gene="dhaK"
                     /locus_tag="L2X49_003156"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBN4798193.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            13499..15418
                     /gene="dhaR"
                     /locus_tag="L2X49_003157"
     CDS             13499..15418
                     /gene="dhaR"
                     /locus_tag="L2X49_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBN4798194.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(15518..18403)
                     /locus_tag="L2X49_003158"
                     /pseudo
     CDS             complement(15518..18403)
                     /locus_tag="L2X49_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin family autotransporter"
BASE COUNT         4440 a         4604 c         4792 g         4576 t
ORIGIN      
        1 gctatgcgag ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta
       61 aatcaggcag gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc
      121 agcgccgcga atgccgggca aatctccccc ggatatgctg caccgtgggc ggcaccaggt
      181 gtgcctttaa aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc
      241 gacggtacgc agttccagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc
      301 cactacatgg aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg
      361 aaagcattgc gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa
      421 ctgttccgta ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat
      481 gccggcgtac cgtatcagct gctggaatcc agccgcctgg cggaagtgga gcccgcgctg
      541 gcagaagtag cgcacaaact gacgggtggt ctgcaactgc ccaatgatga aaccggagac
      601 tgccagctgt ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc
      661 tttaatacgc ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagtgt
      721 ggcgatgaag tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg
      781 atgctcaaag gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt
      841 ccaattgcgc aggaagatgg tgcgccagta tccactattc ttgatgaaac ctacaaaatc
      901 gccattaccc gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt
      961 aacaccgagc tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat
     1021 ccacgcggcg gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca
     1081 gacggtacgc cggttgtcgg gcgtacacgc tttaaaaatc tgtggctgaa taccggtcac
     1141 ggcacgctcg gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct
     1201 ggtcgcacgc cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt
     1261 acgccatcac gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt
     1321 ccgatacagg ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag
     1381 gccgcgccgc acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt
     1441 gagcgtatct ggagcgcgct cggggccacc gatggctttg cattacttaa cctggaagag
     1501 gcaataacgt tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc
     1561 catgctcagg atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac
     1621 tggcagctca aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa
     1681 gtgaacagtg ggatgaatcg gttgggcttc cagcccgatc gcgtgcttac cgtctggcag
     1741 cagttgcggg caatggcgaa tgttggcgag atgaccctga tgtcgcattt tgccgaagcg
     1801 gaacatcctg atggaatttc cagcgcgatg gcgcgtattg agcaggcggc ggaagggctg
     1861 gagtgtcggc gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt
     1921 gactgggttc ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat
     1981 atcgccaata ccggattacg tccggtgatg actctaagca gtgagattat tggtgtccag
     2041 acgctaaaag cgggtgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag
     2101 cgaatcggca ttgtcgccgc agggtacgcc gacggttatc cgcgccacgc gcctaccggt
     2161 acccctgttt tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg
     2221 ctggcggtcg atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg
     2281 ggcaaggaga tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg
     2341 atgtgcgcgc tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc
     2401 ggagacagcg tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt
     2461 gttaatgtga cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc
     2521 ccggaacctg aactcaccgc acaggcgttc tacataaaac gcttacgctt cattgttgac
     2581 tcgaactcga cttcagataa atcacgcttt acccttgatg gagcctgtac atagatttgt
     2641 gtaattgcct gattttgata tgttcaattc aacatcaaat gaaggttaaa ttatggacga
     2701 caaacaattg caggctcagg ctgcgttcag caaagcatcg caaccggcga tagatgcttc
     2761 attaaattta agattcagct tcctcttcag ccacccgtac gccaatcttc aacacttcat
     2821 tatcttcttt ctcggccacc gtccagatca tcccggcaaa ctctacctgg tcaccgacaa
     2881 ccggtgccgc gcctaacaac tgctggacaa tttcacccag cgtttgctgc ttatcacgat
     2941 attctcgccc gtcttccagg ccatatatca gcgcaacatc agcatatttg gcgctggctt
     3001 cgagaatgaa gtcaccaaag aagcgttgat ccagcgcgac cggcggcgac tggctgaaca
     3061 gtttaccgag cgccggaaga tcgcgttccc gaccaattac acacaacaca tcgccttcgc
     3121 gcagtcgggt gctgccggta ggatgaagca actggttatc acgaaacagt gccgcaatac
     3181 gcgtctcttt tggcatatgc aaatcgcgca gtgccgcgcc cacgcaccat ttatcggcac
     3241 tcaattgata aacaaactgc tcccacggat tttccggatg aatatccagg ccaacgcgtg
     3301 acaccggacg tcccactggc ggaaccacca ctttggcttt tttagccgcc cacgagagtg
     3361 atgttccctg caagagcagt gaaaccagaa ccacaaagaa ggcgacatta aagaacagac
     3421 gtgcattctc cagccccgcc atcatcggga acacggccag gatgattggc accgcgccgc
     3481 gtaatcctac ccagctgata aacacgcgct cacgcagatt gaaaccgcgg aaggggagca
     3541 atccagcaaa taccgaaagg ggacgggcga agaatatcat ccatgccgac aaaatgagcg
     3601 ccggaatggc aataggcagc agatcgcttg ggttaaccaa tagccccagc accaggaaca
     3661 tggcgatttg cgccagccag gcgaggccgt cgaaattttg caggatgccg tagcggttgc
     3721 gaatcggccg attacccagc agaaaaccgc acagataaac agccagaata ccgctgcctt
     3781 ccagcgccgt ggttaaagca aaaattaaaa taccgccgct taatgccagc aatggatata
     3841 atccggcagg cagggcgatg cgattaatca tttgcagcag taaataacca ccgccaagtc
     3901 cgatgacaat cccgaggcca aattgctgca gaatatcgac cacgaacatc caactgacac
     3961 tgctttcatg ttgctggatc atcgcaatca gggtaatcgt cagaaagacc gccattggat
     4021 cattactgcc ggattcaatt tccagcgtcg agccaacacg ttcgttaagc cccttaccac
     4081 ccagcagaga aaagaccgct gcagcatcgg tagagccgac gatagcgccg attaataagc
     4141 cttcaatcaa atcaagatta aacagccacg ccgccatcat gccggttaaa ccagaggtga
     4201 taagcacgcc cagcgtcgcc agcgacagtg ccggtcctaa cgccacacga aaggaactgg
     4261 cctgagtgcg catcccgccg tcgagcaaaa taatcgccag tgccaggtta cttaccatgt
     4321 aggcgaaggg gtaattatca aacgggatac cgccgacgcc atcgactcct gccagcatgc
     4381 cgatcgccag aaaaataacc agaataggaa tgccaagacg ggaagaaaat gaactaagta
     4441 atatactgct ggtgacaagg atggatccta agatgaaaag gctaattatt gttgtggcat
     4501 ccaacggtcg gttactcctg attacgctgt ctcttatata aaccctacca tattagcggc
     4561 agagacagcg ttttacttag ccctgaagcg tgattttttt acattttaag aacaggatga
     4621 ccgctaatcg tcaactgagt gccttcccgg gtgttattca gaatggcatg tgcgcccaac
     4681 ggcagcgtaa ccgtgcgttg ttcatgacca aaatcgaggc cggtaatgag tggaatcgac
     4741 aggcgggaac gcaaaaacgc gtacactgac tccaggttgt aacctgcgtc ataatcattg
     4801 ggcgtgctgc cgctaaagct accgagaata atcgccttct gacgtggcaa aattcccgca
     4861 tgatagagct gcaacagcat acgttcgacg cggaaagggt gctcgttaat atcttccagc
     4921 accagaatac cgttctcaat ttttggcatc cacggtgtac caatcagtga aatcagcatc
     4981 gcaagattgc ctccccacaa cgtgccttcg gtctgacatg tcgggccttc cccttgccat
     5041 tcaatggtga aggttttatt gcgtaacgcc agccagaaat ggtgctcggt aaaggcgttc
     5101 agttcatccg caccaaagtt tgctaccagc atggggccgc taaaggtgat gacattgcct
     5161 tgcgccagaa gaccgcactg aatggcggta aaatcgctat gtccgcaaat gagtaacggg
     5221 tcatgttgtt ggcgagcaac cagtgcctgc cagtcaatat ctgccagtaa acgactggca
     5281 ccgtaaccgc cgcgtacagc cagcacgatg gtgttggggg tagtcagtct ggcaagggaa
     5341 ttgagatctt ccaggcgctc cgtttccgta cccgcaaaac gctcacaacg acgggcaata
     5401 acctcgacgt tattgacctg atgcccctcg tcggtaaggc gatggatacc acgcagcgcg
     5461 gcgtgctgtt taatgcagta acccgatggg gcaattaagt gaaacagaga catggcaatt
     5521 ccttgctgac aacagaaacg aaatgtatat catgccgctt aggtgtgccg ttgtcacctc
     5581 aacggcgatt ccaggctata aggatagaag aagtgaaatt gagatggttt gcctttttga
     5641 ttgtgttatt agcgggttgt tcatcaaagc atgactatac gaacccgccg tggaacgcga
     5701 aagttccggt gcaacgtgcg atgcagtgga tgccaataag ccagaaagcc ggtgcagcct
     5761 ggggcgtcga tccacaattg atcacggcga ttatcgctat cgaatcgggt ggtaatccga
     5821 acgcggtgag taaatcgaat gccattggtt tgatgcagtt aaaagcttca acctccggac
     5881 gtgatgttta tcgccgtatg ggctggagtg gtgagccgac gaccagcgag ctgaaaaatc
     5941 cagagcgtaa tatttcaatg ggggcggctt acctgaatat tctggaaacc ggcccgctgg
     6001 caggcattga agatccgaag gtactgcaat atgcgctggt ggtgtcatac gctaacgggg
     6061 caggtgcgct gctacggact ttctcgtcag atcggaaaaa ggcgatcagc aaaatcaacg
     6121 atttggatgc tgacgagttc ctcgatcacg tagcgcgaaa tcatcctgcg ccgcaggctc
     6181 cgcgctatat ctacaaactt gagcaggcac tggacgcgat gtaaatcagt cgcgcacttt
     6241 gtccgctttt tctcgggctt ctcgctcgag agagaaaata atccgctgta attgccgctc
     6301 caccgtcggg ctgacgttaa gaaaacggaa gctcagacgg ggagtggtga tggtttcatt
     6361 cttgccatca atcactttgc gctcgctgat ggagattaac tgggcgtcaa agtgaaaaac
     6421 accccattgc cccatgttga cttcaatctg agcgaagcgc atgccttctt gtaattcggc
     6481 aggctttgct gtttccagta atgcgcccat gccgcctaac gacaaatcat acaggcggaa
     6541 acgtaacgta ctgttatccg ccagtttggt ctggcaaaaa taaggcggat ggagtggggc
     6601 ggagatgcgg aaatatcggc gtcgttgtac aaaccataag gtgggaggcg gtacggtgat
     6661 aaatgccgga agctgcaagt attcactctg ctgtagttgt tcaacagtaa actcgacttt
     6721 cgcaccctga gtttcggcgg taatggtaat gtgctgtgcc tttagcacgg cgatgttgtc
     6781 ttcggcttga ctgccgaaat ccagcaccag tttatccggg gttattgcca gtattttgct
     6841 gatcagctgc ccgccattcc aactgagacg caaaggaatt gcggctttgt gcaaatcgcg
     6901 taacacgccc aggacggcta acggattttg tttcaggaac tgctcatggt aatgactcac
     6961 gcggaaaaac tcctgactga caaactgtct ttggtttatc ggtcacagtt aacaaaactt
     7021 aatacaaatg cgtgaatatt ttttacatgt tgttcttaaa tcagccgaca tacgcccagc
     7081 atcgatcccg cccttgccta tacttagagc gttgatgtaa gcatttcttg cgtccgattc
     7141 atcgaaacga gggcctgaac atgggaatta ttgcctggat tatttttggc ctgatagccg
     7201 gcattatcgc caagctaatc atgccggggc gtgatggtgg tggatttttc ctgacctgta
     7261 ttctcgggat agtcggtgcg gtggtcggcg gctggctggc gaccatgttt ggcattggcg
     7321 gctccatcag tggttttaat ctgcacagct tcctggtggc ggtggtggga gctattctcg
     7381 ttctgggcat attccgcctc ctgcgaagag aataagattt tcataaggcg gatagcgata
     7441 cagatgccgc tatccgcttt cacatcagaa cgtatactcg acacctgctt tacgggtgaa
     7501 aaaaatcaat atagcacttc gatatccgat aaagtcggtt cccggtaaca acaacagaat
     7561 ccttttttaa ttattgtttc gttgtttttg gtgtgattca gaaaataaat attttagaca
     7621 gtagccagtc aggcgttaaa aatggggcgg atagtgaggc ggttttggct gcgcttattg
     7681 aacatttaga attgatcaat ccttctggca gattaagcca aaatacccgt tcggcaatgc
     7741 ttcagttacg cgaagaggaa tggtctgaat tctttttttg gttgctaaat tctctggagt
     7801 gtctggatta tgtgattatt aatttaactc cagaaagcaa aaaaacgttg atgagtgaac
     7861 accgtaacaa tattcaggta gcaattgatg ctttatatag ccaacgtcgg cgtaaatcgc
     7921 cgggtgatga aagtgaaaca cttacccgta gaaatgacgc tatcttcggc aaccacgtat
     7981 ggcaaacatt tgcgcaatac ttcccgccag gacttgaaaa accgtctgtg tagaaaaaac
     8041 catagctatg gtgttatctg ccgacaacgc catccggaga agccgaatga cgttgtcgga
     8101 gtaagcgctg gttaaggtgt gggttgtgcc tctttggttg agggttgcgt cgttgctgac
     8161 ttaacggtcg gacgcgtcgc cggaacattg tcacacggtt gctctttcgg gcagatcaaa
     8221 tccagcattt tcagcgtcac gccattggtc cagccaaagc catcctgtaa tggatattcg
     8281 ccaccgccgc cccccgttcc ggtggtgctg acatcatatt tttccaccag ctttttctcc
     8341 cggtcatagg tgtgctgaac attggtcagg aagtgccagc taatgtccat cgccacctct
     8401 ttttgcccgt agttttgtaa tccttctgtc gcgacccact gtaacggtgc ccagccattt
     8461 ggcgcatccc attgttgccc acttttcacc gacgtggtgt tcaggccgcc gggttgcagc
     8521 agatgtgttt tcgtcgccgt cgccattttg ttggcgcgat ctttcgctgc cgcattgacg
     8581 tacagcggga acagggcggc cgcggttaac tgattgcgca ctttatgact tttcaggtcg
     8641 taatcggcat accagccttg ttgatcgttc cacatgtatt tttcgatccc tttttgacgg
     8701 gcatttgcca gcgtttcgta ctggtttgcc atcgcgttat ctccggcagc tttgctggcg
     8761 cgggcgagga ttttttccat tttaaacatc aggctgttca gatcgaccgg tacgatgctg
     8821 gtggtgcgta aggtatttag ttgatgcggg ttgtccatcc agcgcgagct gaaatcccag
     8881 ccagacgcag cggcagagcg caggtcgcgg taaatttcag tggcaggtcg attcggattg
     8941 cttttggcgg tggcaatatc ttccacccat gactctggtc gtggcgtatc gcgatcgtcc
     9001 cagtagcggt tgagaagggt accatcctga agttttacaa cgcgtttttc ctgttgtccg
     9061 gcttgcaggt tttcaacacc gtccatccag taagcatatt ctttttgcat ttgcggcagg
     9121 tattgcttca acgcggcatc gccttcatgc tgcgccagta actctaccat cagggcaaag
     9181 aagggcggtt gcgagcggct taaatagtaa ctgcggttgc cgttgggaat atgaccgtaa
     9241 gtgtctattt catgagcaaa attggccacc atatccgcga ctttatccca gtgaccgctt
     9301 tcggcaagtc ctaacatggt gaagtaactg tcccagtaat atacctcgcg aaaacgtccg
     9361 cccggcacga cataaggttc cggcagcggt aacagagaat cccatttttc ggtgttttcg
     9421 gtagaacgcg ttaataccgg ccaaagtccg tcaatatgtt cgcgcagtga ctgcccctct
     9481 ggcggaacat atttctcgcc ttctttcggc agggtgaaat tgacgttaac gaaatggcgc
     9541 agatcaaatc cgctctggtt ttgctgcatc cgataatcag caaggatcat cagcggatcg
     9601 ctgttcggca cggcatcggc aaaggttttt tggtccggaa aaagtttggc gttttgcaca
     9661 tcattaaaca gcggccctaa taaaatatca ggcggctgtg gtgttaccgg tgtttcttct
     9721 gcctgcaccg atagcgcagc gaaacacaaa aagatacagg ctggaattaa cgccattttt
     9781 tgcgggcgag aaggtgcggg ggatttcatc aatcattctc ctttggcgaa accgaataaa
     9841 acggtattca agtatcagaa aaccttagtt caggatcgca ctagctgcat gatcgaaatc
     9901 tcattctgcg aactgtcagg cattttagaa aagggaatta aagggataaa taacggcgaa
     9961 atagagtgat caaataacgt ctgtttatta gccagccagc ggcgttgggt tcgtgattag
    10021 cgaagaaaaa tcatcataaa ttggtgaaaa atataacagg tagttaaaaa ccattagtgc
    10081 tgagtaaatt gccggatgac atcagaacga tgccatccga acagtggctt aaccctgacg
    10141 gttgaaacgt tgccttttaa cgtccagcgt tagcgtttct tctggttgta tcgcatacag
    10201 tttctcaccc tgctggcaaa tccagccaat ccccagttca cgggcgatta gggcgctgtg
    10261 ggataccgga cttccggcgc taaggcagat accttttaca accgccggat ccagttgcag
    10321 tactgtggaa ggataaatgt tctccgccag tagaatagtt ggcgagttaa actgcgggag
    10381 ttcttctttc gtttgggtca ggtggaccag ggtgcgatgc agaagatcgt ccacatcaat
    10441 atagcgagct tgtagatatt catcatccag ttgctggtat tgctggctaa gttctttaag
    10501 aacttgctgc caggcatatt ctgccgtgca atgttcatgc tgaaggagtt cgcttgccgc
    10561 cgccagcagt tccggatcat ctaacagtgt atggtgacca gaaaagattg cggcaatatc
    10621 gtcaagcccg ctggcttctg cttttgctgt taacgtcatc agatctaata acgtgaagtc
    10681 aatagcctgg cgtaatcgat cttgttcttc ttccacggtc agggttgatt ttgcctgtac
    10741 cgtacataaa actggttgat aataaaaggc tttacccgaa acaggcggaa cgggacgcag
    10801 agtaggtgga gcgacttcct ccgtttcacc aaagttatct tcagccagct gacggaaagc
    10861 gatcagtgcc tcttcagctt ctggcccttt cgcaatcagg cgcagcgtat cgttatagcg
    10921 aacttgtagt aacgcaatct ggttaatact ctctggtgtg acgcatttgc cgtttttttc
    10981 cagcaacata tcggcattaa atgtcgataa ggtataaacc agccgggagg ccggacgtac
    11041 atgcaggccg ttacggtttt ttatgaccac cgccagagaa cgggcttctt catcgtacgc
    11101 aggacatgtg tcagagattt tagtgtcgga ggacggtaaa cccagttgtt cacgtttggc
    11161 ttccagcgca tgcatagcgt caaagataac tttgtcgata tccgctcccg aggccgcgct
    11221 gaccgttgct gccagtgtac cttcgaccaa cggcgcagca cacaaacgta cttttgcggc
    11281 gatcttggga gccagcaatt ccagcgcagt ttcagcactc aataatgcgc tacccatatc
    11341 catcatgacc agcacatggt cggcatcagc aacagattcg atggcctcca tcactttgac
    11401 ggcatcggta ccaataggat tttgtggatc gtcaattccc gcggcaatgg cgattttaca
    11461 actatcactc attaacatct gacgggctaa ttcaccgaca ccttctccca gtcggctgct
    11521 atgtgaaact atgaccaggt ttaccatcac caattcctta ctcttttgcg gctaacgcca
    11581 acatttgcat cataaacatc accgaggtcg cgccgggatc ctggtgacca atactgcgtt
    11641 caccgagata actggcgcgg cctttgcggg cttgcatcgt aatcgtactt tgtgcagcgg
    11701 attcggcgat tctactggca gcttcgagcg ccaccggaac agagagattt tgctcgctgg
    11761 actgacgtaa cgattccacc accggcaccc acacatcaca catggtttta tcgccaggtt
    11821 cggctttccc gcgactgatt acgccgtcca cgccatcgcg gaacatctga taaagctctt
    11881 ccagtgtcag gctttgccgt gcctgggtcg cctgtgcggc gcggataaag aaggtaccga
    11941 acagcggacc actggcacca ccgacgctgg aaagcagcgt cataccggta ttcttgagaa
    12001 tgaaaccgat atctttatct gcgatagcag ggagtttttc caccactttg ctaaagcctc
    12061 ggttcatatt tagcccgtgg tcagcgtcgc caatttcgcg atccagtccg gtaagatact
    12121 cgctctcggt gctgaaaata tcgccacaac gagtgagcca gttaacaatt tgagttctgc
    12181 tcagtgacat tgctttctcc ttatttaccc cagttaaggg ccggggtgtg gaccggggcg
    12241 tcccagagtg ccagcgtttc gtcatcaact ttcagtaagg tgattgagaa accggtcata
    12301 tccagtgagg tgcagtacgc gccaattaaa ttacgttcga tagtcaatcc cgcttgctgg
    12361 caacgtgtgg tcaggcggtt atagacgccg tacagctcag aaagcggagt tgcgccaaga
    12421 ttgttaacca gcgcaatcac ccgatcgcca gactggagcg gttgtttggt ttgttgttct
    12481 tcctgccaac tgccttgttg ataatcccag aaacgcaaag tgcgatggta tgagccattt
    12541 tccagcaggg tgtcgaacat ttcatcgacg gtttgatcaa gggaagagaa ggagcggcgg
    12601 tcaatacccg gctcaccatg aatgccgacg ccaaactcca tctcattatc cgccagggta
    12661 aaagaaggtt tgcccgcggc aggaacggta caggcaccga gagcgatacc tattgagtgg
    12721 ccttgattat tcaacttacg ccccagttcc gcacaggcgt ccagtgagtc gccacgctcc
    12781 gccgctgcgc ctacgagttt ttcaattaat acggtgttgg caacgccgcg tcgcccggcg
    12841 gtataaaggc tgtcttttac cgcaacgtcg tcatcaatga ccacagtggt cacttttacg
    12901 ccgctatcgt gcagtaactc ggtcgctgtt tcaaagttaa gaatatcgcc ggtgtaattt
    12961 ttgataatca acagtacacc ttcgccgcca tcaatttgca tggagcattc aaagatttta
    13021 tcgggcgtcg gtgaggtgaa aatttcgccc ggacaggccc ccgaaagcat cccctgaccg
    13081 atataaccac agtgcatcgg ctcgtgtccg ctgccgccac ccgacagcag ggcgactttt
    13141 cctgcaacag gggcatcagc tcgggtgaca tacaccggat cctgatgcag tgtcagcgat
    13201 ggatgcgctt tcgccagtcc tgccagttgt tcgtccagta cgtcttgcac atcattgatc
    13261 aattttttca ttattttgct ccagcaatta cggtagggca tggatgatgt tcaacgacac
    13321 ggcgaccact gactgccgat gaatccattg tgcatcaggc aagggaaaag aaaattagcc
    13381 caaattatgt ttcatagtga aacatatgct ttaatgaatg ttccatattg aaacttttac
    13441 gtgtattaat acttaaaatt gcgagccgga acaccttttg tcataaggga tgcgggatat
    13501 gagtggcgct tttaacaacg atggtcgggg catatctccc ttaattgcaa cctcctggga
    13561 gcgatgcaat aagctgatga aacgggagac atggaacgta ccacatcagg cccagggcgt
    13621 gacatttgct tctatttatc ggcgtaagaa agcgatgctg acgctcgggc aggctgcgct
    13681 ggaagatgcc tgggaatata tggcaccgcg agagtgtgcg ctgtttatcc tcgatgaaac
    13741 cgcctgcatt ctcagccgta atggcgatcc gcaaaccttg cagcagctaa gtgcactggg
    13801 attcaatgac ggcacgtatt gcgccgaggg aattattggt acttgtgcgc tatcgttagc
    13861 ggctatctct ggtcaggccg tgaaaacgat ggccgatcaa catttcaaac aggcactctg
    13921 gaactgggcc ttttgtgcaa cgccgttgtt tgacagcaag ggccgattga cgggaacaat
    13981 agcgctggcg tgtccggttg agcaaactac cgcagctgat ttgccgttga cgttggcaat
    14041 cgcccgcgag gtcggaaatt tactgctgac ggacagtttg ctcgctgaaa ctaaccgtca
    14101 tttaaatcaa cttaatgccc tgttagaaag tatggatgat ggcgtgatta gctgggacga
    14161 gcagggtaat ttgcaattta ttaatgccca ggcggcgcgg gtcttgcgcc ttgacgcgac
    14221 ggcaagtcag ggaagggcaa tcactgaact cttaacgtta cccgccgtat tgcaacaagc
    14281 aataaaacag gcacatccgc tcaaacacgt agaagcaacc tttgaaagtc agcatcagtt
    14341 tattgatgcg gtgataaccc ttaaaccgat aatagaaacg cagggaacca gctttatttt
    14401 gttgctccat cctgtggaac agatgcggca gttgatgacc agtcaattag gaaaagtcag
    14461 ccataccttc gctcatatgc cacaggacga tccgcaaacc cgccgcttga ttcattttgg
    14521 tcgccaggcg gcgcgcagta gctttcctgt cctgctttgt ggagaagagg gcgtgggaaa
    14581 ggcactgcta agtcaggcaa ttcataatga aagcgagcgt gctgcaggtc cttatatcgc
    14641 cgtcaattgt gagttatatg gtgatgcggc gctggcggaa gaatttattg gtggcgatcg
    14701 cacggacaat gaaaatggcc gtctgagtcg gctggaactg gcacacggcg gcacgctgtt
    14761 tcttgaaaag attgaatatc tggcggtgga gttacagtct gctttgcttc aggttatcaa
    14821 gcagggtgtt atcacgcgac tggatgcgcg gcgtttaata ccagttgatg tcaaagtgat
    14881 tgccacaacg accgcggacc tcgcaatgct ggtggaacaa aatcgtttta gtcgccagct
    14941 gtattacgcg ttgcatgcat ttgaaattac catcccgcct ctgcgtatgc ggcgtggcag
    15001 cattccggcg ctggtgaata acaaattacg cagtcttgaa aaacgcttct ctacgcggct
    15061 gaaaattgat gacgatgccc tcgctcgcct ggtttcctgt gcatggcctg gcaacgattt
    15121 tgaactttac agcgtcatcg agaatcttgc tctgagtagt gataatgggc gcattcgcgt
    15181 cagtgatttg ccggaacatc tgtttaccga gcaggcaaca gatgatgtca gcgccacacg
    15241 cctttccacc agtctgtcat ttgcggaagt tgaaaaagag gcaattatta acgcagccca
    15301 ggtcacaggc ggtcgcattc aggaaatgtc ggctttactt gggatcggcc gcactacgct
    15361 gtggcggaaa atgaagcaac atggcattga tgcagggcag tttaagcgcc gggtatgaaa
    15421 gacagagacg atttctgata catcagagtg atctatattt cattccggcg cacgctaaca
    15481 tttttcagca tcgtttaagg gctggtctat cccgcactta aaagctgaag cgatatcctc
    15541 cgttgacttg cttctgatca aagaggttac tctgcgtgta atccgcttcg aggtagaatg
    15601 tgtgctgttt gttatactgt gcactgactc ccacgccgtt attccagcca ttacctttga
    15661 aactgtactt ctcccgggag ttgttcaaca gatagtctgt atcccctgaa aactcgcgga
    15721 tagctccagt cttcacatac atattcagct ggctgttgcc tgcggtgatg tcatatccca
    15781 gtatcatgct ggcacgcccc agcagcgatt cgtagtgatt cagatgtata ttgagaccat
    15841 tactcgcctt catagccatc tcattctggt ggctgtatgt gagctgggtt tgcggctcta
    15901 tatagaaccc ataaccagta ggggtcaggt tgaacctctg cccggcttcc agggagatgc
    15961 tcaatccatt cgcagtgccg ttggcgttaa cgccgttgtt ctgactgtcc agtacgtgaa
    16021 aactattttt ctggcgcgat gcttttacaa cgagatcgct gtaaaaaccg ttgtgtgcca
    16081 tgtaactggc atacattccc atgtagtctg aacgtgcggt accgtcgcct ccgctatagt
    16141 ccggcgatgc atgtgttgag ccaatataca gaccgacata caacggcatt acatcagaga
    16201 gacgtttatc cccaccaaac tggatactgc tgtaacccat gtcaaagccg ctcagtttgc
    16261 cactggcgaa ggagtccagg cttcccccat aactgcgcag ccagatatta ccgtctttac
    16321 tctgatttcg caggtcaccc attcgttgca tcagcgtacg gttttcaaca tagtttaata
    16381 agtaaccgac attgagataa ttgccgccag catctgcggt cgtggtgggt ttaggtgtgg
    16441 gagtggggtt aggcgtagga tcgggttcag gcgtaggatc ggggttgact atgggaggct
    16501 gagcgggagc cggtgttggt tcaggattag gagtcggttc cggaactgtc ccggaagcgt
    16561 aaagctccca gttagtgcca tttttacgca catcgtacag atatcccccc aactcaacct
    16621 gagaagacgc gctgaacgag gccgcaccgt cagtggtttt taccactgtc agaacttcat
    16681 ttcccgtagt ggcctcgctg ccctggttgc ggatagccaa tacgtgatta ccagcactac
    16741 ttccgctgat attcaataaa tcccctttat tattaacgcc attaccctcg ccaacaacat
    16801 cagcacgcat aataaaggta ctgttaccgc tcaggttctc tacgtttaat gtggcaaatg
    16861 tgccggcagt tgacccgtgg ctggcaaaat cgacagttga atggctcagc gccagcgtgt
    16921 cgagattaga attacttgtt acgttccaga cgctgttatt cattgcaacg tccagtttcc
    16981 cgccattgac attatcgctg agggaggaac ctgtccaaac cgaccctggg tgcatatcta
    17041 gattgattag cccacctttg gaaagaacgc taccgttgat aagcatacga cccgaggcgt
    17101 tgatgcggct ggcggcataa ccatcgtcat gttacgttgc aatggccatc tggtcgggtg
    17161 tactcatatc aatgaccaga tcgctcgtga ggtcgatctg gctgttggtc atggcataaa
    17221 ttcccctggc tcctgccgcg ccggtgatag ccaaactgtc tccggttatt ctaccgccgc
    17281 tgagcgccca caaacccagc gccagactgc cattacgatc aactgtaata tcggtatttt
    17341 gcatgttgac aacagccgtt gccgtctggg ccgaggcacc ataagaaccg ccggaaaaga
    17401 tgctgtttcg ttgcgctgcc gtgccagaaa aattgattgt cgcgtctgaa ccactggtga
    17461 cgaggccacc gccctgcgcg gaagaaatat ggctatctgc accgatagtg gttgtaccac
    17521 cacgaacttc gacgccattg gccgcggctc cagttacatc aacagtgagt gcattcgcgc
    17581 taacctggcc aaagctccag aggccgtgtg cattatcgcc attggtttta atggaactgt
    17641 ttgttccgag atcgacaaca gagtttttct gtacgtttat ccccatggcg ctgtagccct
    17701 gaacatcgat tgtcaggtct gtagccgtaa aacgcgcagc accattggcg ttattgccgt
    17761 tgagaccacc gatataaaca cctgtacttc catcggtcgt gattttactt ccgcttccaa
    17821 gatcgatact ggtgccatag tcattgatgg ttaggcctat accgttcgag ttttcaatgg
    17881 tgaattgagt ggctgttagt gttgagctat gcccaataat gatgccgtca tcgttactct
    17941 taatggtgct gcctgtgcct aagtcagcat gggtatagtc accaattaag ttaatgccga
    18001 tggcagaggt ttgaccaaca acatctactg tgagtcggtt agctgtcagg gagctgtttt
    18061 tgccctgaat aatgattcct tttgcatatc cagaggcgtc gttgacattg acggtgacat
    18121 cattaccgag gttgatattg ccaggcgtgt ttcctgctgg ggtcattaca ccatagaggt
    18181 ttcctgatgg atcggcggta tctcctgtaa ttctgtcacc ttcattaata gtaatattgg
    18241 tgccatattg acttcctgta tcagcagcta tcagcagggg ggaaaagata attgcattca
    18301 aaccaatagc gccaaatttt atcagttgaa ttgagggcga acggattttt aattcagcga
    18361 gacgatctgc tttggtattc ccattgtgtt gtttgatgcc cataccactc ct
//
LOCUS       DAFNBJ010000098        17926 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000098 DAFNBJ010000000
VERSION     DAFNBJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.098"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(148..1740)
                     /gene="lsrK"
                     /locus_tag="L2X49_003159"
     CDS             complement(148..1740)
                     /gene="lsrK"
                     /locus_tag="L2X49_003159"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HBN4798195.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDIYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1819..2772)
                     /gene="lsrR"
                     /locus_tag="L2X49_003160"
     CDS             complement(1819..2772)
                     /gene="lsrR"
                     /locus_tag="L2X49_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBN4798196.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3021..4556
                     /gene="lsrA"
                     /locus_tag="L2X49_003161"
     CDS             3021..4556
                     /gene="lsrA"
                     /locus_tag="L2X49_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBN4798197.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4550..5578
                     /gene="lsrC"
                     /locus_tag="L2X49_003162"
     CDS             4550..5578
                     /gene="lsrC"
                     /locus_tag="L2X49_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBN4798198.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5578..6570
                     /gene="lsrD"
                     /locus_tag="L2X49_003163"
     CDS             5578..6570
                     /gene="lsrD"
                     /locus_tag="L2X49_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBN4798199.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6582..7604
                     /gene="lsrB"
                     /locus_tag="L2X49_003164"
     CDS             6582..7604
                     /gene="lsrB"
                     /locus_tag="L2X49_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HBN4798200.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7631..8506
                     /gene="lsrF"
                     /locus_tag="L2X49_003165"
     CDS             7631..8506
                     /gene="lsrF"
                     /locus_tag="L2X49_003165"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBN4798201.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8530..8820
                     /gene="lsrG"
                     /locus_tag="L2X49_003166"
     CDS             8530..8820
                     /gene="lsrG"
                     /locus_tag="L2X49_003166"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBN4798202.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8877..9635
                     /gene="tam"
                     /locus_tag="L2X49_003167"
     CDS             8877..9635
                     /gene="tam"
                     /locus_tag="L2X49_003167"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBN4798203.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9639..10553)
                     /locus_tag="L2X49_003168"
     CDS             complement(9639..10553)
                     /locus_tag="L2X49_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBN4798204.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10760..12211)
                     /gene="uxaB"
                     /locus_tag="L2X49_003169"
     CDS             complement(10760..12211)
                     /gene="uxaB"
                     /locus_tag="L2X49_003169"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBN4798205.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12438..13855)
                     /locus_tag="L2X49_003170"
                     /pseudo
     CDS             complement(12438..13855)
                     /locus_tag="L2X49_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(13994..14353)
                     /locus_tag="L2X49_003171"
     CDS             complement(13994..14353)
                     /locus_tag="L2X49_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBN4798206.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14353..15279)
                     /gene="glsB"
                     /locus_tag="L2X49_003172"
     CDS             complement(14353..15279)
                     /gene="glsB"
                     /locus_tag="L2X49_003172"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBN4798207.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15343..16731)
                     /gene="sad"
                     /locus_tag="L2X49_003173"
     CDS             complement(15343..16731)
                     /gene="sad"
                     /locus_tag="L2X49_003173"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HBN4798208.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16832..17713
                     /locus_tag="L2X49_003174"
     CDS             16832..17713
                     /locus_tag="L2X49_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4798209.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17791..>17926
                     /gene="yneK"
                     /locus_tag="L2X49_003175"
     CDS             17791..>17926
                     /gene="yneK"
                     /locus_tag="L2X49_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBN4798210.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     E"
BASE COUNT         4490 a         4594 c         4477 g         4365 t
ORIGIN      
        1 gcaattttcc cttgaatata gtactggtga tggaacgatg aatctgcaag ctggctttta
       61 acaagccagc tctaaaagaa gggaaataag aataactata ctcaaaaact aacagccacg
      121 gtcatcatga tgtggctgtc aatgaaacta taacccaggc gctttccata acgacgtcgt
      181 cagtccatga tcaaccagcc ccagctgatc ctgataaact gcctgccact tatcgcgtga
      241 atcctgataa agttcatgct tttccgggtc tggtgtgtgc gtccgttccc agcgaaccag
      301 gcgttctccg gtttctgcca ttgatgaaaa aattccggca ccgacgccag ctgcaatggc
      361 acatcctaat gcagtggctt ctttgaccac cggaatattg acgggtaatc ccgagacatc
      421 agcgagaatt tgactccata atttcccttt tgaacctccg cctgcaaaga ctaacgatga
      481 aggatgaata ttcgagaaat cagcaatttg ctgcaagtta cacgctgata caatcgccgc
      541 attttcttcc agcgcacgga acaatgtcgc tttgttacat ttatccgggt caatggacaa
      601 gttaataaag gaaggcgcag cgtgatacca ggttttaaag cgcattctgt cggagaagat
      661 cggcattacg ccccacgacc caggcggcac ccgactggcc atctcttcca gcagcgtata
      721 gatgtcgatg cctaaacgtt cggcaatcag tttttcttcg gcacagaaag catcgcggaa
      781 ccagcgcatg gtgagtccgg taaaaaagct tatagattca gcttgtacca tgccaggaat
      841 aacatgagga ttaacgcgca cgttcatttc tgggtctgtc accggcgcgg ctaaatttac
      901 aacttgctgc cagaatgtgc cgccaagaac cgcggtttgt gccggacgca caacgcctaa
      961 cccaaggcaa ccaagctgca cgtcgcctcc tccaacgacc accggagtgc ccgccttcag
     1021 accgcagagt tccgccgctt gtgaacttac cacgcccagc aatgtgccgg tttctttgac
     1081 aggagaaaga atatcggcac gtaggccagc catatccagc aatgcaggtt tccagtcacg
     1141 ggtggttaga tcaagaagtc ccgtggtgcc agcgttagag ggatccaccg ccagttcgcc
     1201 gctgagcata taggccagcc agtcgctgat catggtgatg gttgatgcct gacggtaaat
     1261 atcggaacga tggtgcgcca gccaaagtaa tctggggatg gcacttaaag ccagtgtttg
     1321 tccggtcgcg cgataaactt cgttttcaaa ggtattgttg tgcagttctt taagttcgct
     1381 aacttcgcgt gccgctctgg catccacatt ggcgcaggcc cagatcgggg ctccttcatt
     1441 attatataaa acaatgcctt cacgcatcga acatgccgaa acggcagcga tatactccgg
     1501 ggctatgccg gcgttgtgca gcgcctggcg catacactca cacgccagtt gccagttttt
     1561 gttgagatca aattccatag aaccaggaac gtccggtact gccaggtgcc gccactccgc
     1621 ctgtcccact gctatttgat tgccttccag gtcgaatatc acagcccgaa tacttccggt
     1681 acctgcatcc agcgccatca ggtaatactt tgattctgaa ggggtaaaga gtcgagccat
     1741 tagattatcc tcggcttatg cgttctgtct gttcctctat acgttctcca tcattcccgg
     1801 taataaggtc atgcaaattt aactacgtaa aatcgccgct gctgtgtcct gatcggtaac
     1861 cagtgcgttg atataaccgc ctttcattgc agcggcaatt gcttcggctt tattttctcc
     1921 ccctgccacg ccaacccgga cgggtatggt cttcagcgag cttaaaggta agccaatcag
     1981 ttcgttatgt attttaatat ccgtgacaac gtcacctttt gcatcaaaaa agtagcctaa
     2041 aatgtcgcca accgcccctt ttcgaccaat cattaactgt tcgccctggc tgatataacc
     2101 ggagcgaatg atcgtcgcgt cgtcctgttg actgacggcc ccaatgccga caatcgccac
     2161 atcggctgct tgcgcggcta acagaacatc tttgacgcaa ttttcatttt ttagcgtacg
     2221 ggcaatgtca gcggaggatg cccgcaacgg agccggaata atattcacac tgcacgccgc
     2281 gttaagctgc ccgatacccg tcatataaga accgacgcca ccggagagcg tgaccaggcg
     2341 aatttgctgt gacgaaataa aaccacttaa gcgttgcagc gtattcatgg ttgcctcgcc
     2401 aaaaccaatc gccagcatct gttgtggttg aagtaaactc atcaacatat gcgccgcgcc
     2461 tatccccagt cgcccgccga catcagcatc cgcaagccca gggatcaccc ggacatgttg
     2521 cagcgaaaac tgacgacgta attgagtttc atattccaga cagccttcaa agcgagaatt
     2581 aatctgtacg cgaataatgc cggactgatg ccctttctcc agcaagcgcg acactttcaa
     2641 acgggtcagg ccgagacgat cgctgatctc gctctgggtc agcccgtcgt gatagtaaaa
     2701 ccacgcgatc cgcgcgacct gttcttcttc acacattccc tgttctgaaa ttgccgaatc
     2761 gttgattgtc ataattcatt cttcactttg aacatattta aatctttaat gcaattgttc
     2821 agttcttgcg catttatatc tgtgatggca gccacagttt gactctacga gcatgaacaa
     2881 acgcaaccgt gaaaatcaaa atagcataaa ttgtgatcta ttcgtcggaa atatgtgcaa
     2941 tgtccaccta aggttatgaa caaattaaaa gcagaaatac atttgttcaa aactcacctg
     3001 caaaactgaa cgggggaaat atgcaaacga gtgatacccg cgcgttaccg ctactttgcg
     3061 cccgctcggt ttataaacag tattcagggg tcaatgtcct gaaaggcatc gattttacgt
     3121 tgcatcaggg ggaggtccac gccctgctcg gcggcaatgg tgccggtaaa tcgacgttaa
     3181 tgaagattat tgccggtatt acccctgctg atagcggtac gctggagatt gggggcaaca
     3241 actacgccag attaacgcca gttcatgctc atcagctggg catttatctc gttccccagg
     3301 aaccgctgct tttcccaagc ctgtcgataa aagaaaacat cctgtttggg atggcaaaaa
     3361 aacagctctc catgcaaaaa atgaagaact tgctggcggc gctgggctgc cagtttgatc
     3421 tgcatagtct ggcaggatcg ctggatgtcg ccgatcgcca aatggtggaa atcctccgcg
     3481 ggctgatgcg cgactcgcgg attctgatcc tcgatgaacc taccgcctcg cttacccctg
     3541 cggaaaccga acgcttgttt agtcgcttgc aagagctgct tgctactggc gtgggtattg
     3601 tttttatctc gcataagctg ccggaaattc gccagattgc cgatcgaatt agcgtgatgc
     3661 gcgacggaac catcgcctta agcggcaaaa ccagcgaact gtctaccgac gacattattc
     3721 aggccatcac cccagcggta cgggaaaaat cgctctctgc cagccaaaaa ttatggctgg
     3781 agttacctgg taaccgccca caacatgctg ccggaacgcc ggtgctgaca ctggaaaatc
     3841 tgaccggcga aggtttcagg aatgtcagcc tgacgctcaa tgccggagaa attctgggcc
     3901 tggctgggct ggtgggagcc ggacgcacag aactggccga gacgctctat ggtctgcgta
     3961 ctttgcgtgg cggacgcatt atgctgaatg gtaaagagat caataaatta tccactggag
     4021 aacgtttact gcgcggtctg gtttatctgc cggaagatcg ccagtcatcc ggactgaatc
     4081 tcgatgcttc gctggcctgg aacgtctgcg cccttactca taaccttcgt ggattctggg
     4141 cgaaaaccgc gaaagataat gccaccctgg aacgttatcg tcgggcgctg aatattaaat
     4201 ttaaccaacc ggaacaagct gcacggacat tatccggtgg caaccagcaa aaaatcctca
     4261 ttgccaaatg cctggaagct tcgccgcaag tattgattgt cgatgagccg acgcgcggcg
     4321 tggatgtctc ggcacgtaat gatatctacc agctgttgcg cagcatcgcc gcacagaatg
     4381 tggctgtgct gcttatctcc tctgatctgg aagagatcga actgatgaca gatcgtgtgt
     4441 atgtgatgca tcagggcgaa attacccact ctgcactgac cgggcgcgat attaatgtcg
     4501 agactattat gcgcgttgcc ttcggcgata gtcagcgtca ggaggcatca tgctgaagtt
     4561 tattcagaac aaccgtgaaa tcacggcact gctggcggtg gtgctgctgt ttgcattacc
     4621 cggttttctc gaccgccagt atttaagtgt gcaaacgctg accatggttt atagcagcgc
     4681 gcaaatcctg atcctgctgg caatgggcgc gacgctggta atgcttacgc gcaatattga
     4741 tgtttcagtg ggttcgatta ccggaatgtg cgcagtgctg ttggggatgt tactgaacgc
     4801 ggggtattca ctacctgttg cttgtgtcgc gactttactg cttggtttgc tcgcgggatt
     4861 tttcaacggt gtcctggtcg cgtggctaaa aatccctgcc attgttgcca cccttggcac
     4921 gttagggttg tacagaggca tcatgttgct gtggactggc ggtaaatgga ttgaagggtt
     4981 acccgccgaa ctgaaacagc tctccgcacc gctgctgttt ggcgtttccg cgattggttg
     5041 gttgacgata attctggtgg catttatggc ctggctgctg gcaaagacgg cgtttggacg
     5101 cagtttttat gccacgggcg ataatttaca gggcgctcgt caactgggtg ttcgtacgga
     5161 agccattcgc attgtggcat tttcgttgaa cggctgcatg gcggcactgg cgggaattgt
     5221 gtttgcttcg cagattggtt ttatccccaa ccagaccggt accgggctgg agatgaaagc
     5281 aattgcagcc tgcgtgctgg gcggcattag tttgctcggc ggttccggtg cgatcatcgg
     5341 tgcggtactc ggcgcatggt tcctgacgca gatcgatagc gtactggtgc tgttgcgcat
     5401 tccggcatgg tggaacgatt ttatcgcggg cctcgtgctg ctggcggtgc tggtgtttga
     5461 tggacgcctg cgttgtgcgc tggaacgtaa tctacggcgg caaaaatatg cccgctttat
     5521 gacgccaccg ccatccgtta aacccgcttc gtcaggtaaa aaacgggagg ccgcataatg
     5581 cgtattcgct acggttggga actggctctt gccgcactgc tcgttattga gattgtcgca
     5641 tttggtgcaa ttaacccgcg aatgttagat ctcaatatgt tgctgttcag caccagtgac
     5701 tttatctgca ttggcattgt cgccctaccg ctgacgatgg tgattgtcag tggcgggatc
     5761 gatatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt actgtttcaa
     5821 agtggtgtgc cgatgccgct ggcgattctc ctgaccttac tgctcggcgc attgtgcggg
     5881 ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat tacgcttggc
     5941 acgctgtatc tgtttgccgg aagcgcccta ctgctttccg ggatggccgg agcgacgggg
     6001 tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct ggatgtgctg
     6061 ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg gctctggctg
     6121 cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg cgtggcgctt
     6181 tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac ggggctggcg
     6241 tctgcggtcg ccgctgtgct gctggtgtcg tattttggtt cagcacgttc cgatctcggt
     6301 gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggtgccaa tatttatggt
     6361 ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata tttgcaacaa
     6421 ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg tgcgctactt
     6481 atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa agagtggctg
     6541 gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt catcgcttta
     6601 agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat gtgcaggccg
     6661 cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc agcggtggca
     6721 acggcgcaca acaagcgggt aaagagctgg gcgttgatgt gacctacgac gggccgacag
     6781 aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa ggttataacg
     6841 ccattatcgt ttctgcggtt tcgcctgatg gcttgtgtcc ggcactgaaa cgcgccatgc
     6901 aacgtggtgt gagagtgctg acctgggact ctgatactaa accggagtgc cgctcttact
     6961 acattaatca gggaacgccc gcccagttgg gaggtatgtt ggtggatatg gcggcgcgtc
     7021 aggtgaataa agacaaagcc aaagtcgcgt ttttctactc aagccccacc gttacggacc
     7081 aaaaccagtg ggtgaaagaa gcgaaagcga aaatcgccaa agagcatcca ggctgggaaa
     7141 ttgtcactac gcagtttggc tataacgatg ccactaaatc gttacaaaca gcagaaggaa
     7201 tattaaaagc gtatagcgat ctcgacgcca ttatcgcccc cgatgccaac gccctgcccg
     7261 ctgccgcaca agccgcagaa aacctgaaaa atgacaaagt agcgattgtc ggattcagta
     7321 cgccaaatgt gatgcgcccg tatgtagagc gcggcacggt gaaagaattt ggcctgtggg
     7381 atgtggttca gcaaggcaaa atttcagtgt atgtcgcgga tgcattattg aaaaaaggat
     7441 caatgaaaac gggcgacaag ctggatatcc agggagtagg tcaggtggaa gtctcgccaa
     7501 acagcgttca gggctatgac tacgaagcgg atggtaatgg catcgtactg ttaccggagc
     7561 gcgtgatatt caacaaagag aatatcggca aatacgattt ctgatgtgca ttacttaacc
     7621 ggagtaagtt atggcagatt tagacgatat taaagatggt aaagattttc gtaccgatca
     7681 accgcaacaa aatatccctt ttaccctgaa aggttgcggt gcgctggatt ggggaatgca
     7741 gtcacgctta tcgcggatat ttaatccgaa aacgggtaaa accgtgatgc tggcttttga
     7801 ccatggttat tttcagggac cgactaccgg acttgaacgc attgatataa atatcgcccc
     7861 gctgtttgaa catgccgatg tattaatgtg tacgcgcggc attttgcgca gcgtagttcc
     7921 cccggcgacc aataagccgg tggtactgcg ggcgtcaggt gcgaactcta ttctggcgga
     7981 attaagtaat gaagccgtgg cgttatcgat ggatgatgcc gtgcgcctga acagttgcgc
     8041 ggtggcggcg caggtttata tcggcagcga atatgaacat cagtcgatca aaaatattat
     8101 tcagctggtc gatgccggaa tgaaagtggg aatgccgacc atggccgtga ctggcgtggg
     8161 caaagatatg gtgcgcgatc agcgttattt ctcgctcgcg actcgaatcg ccgctgaaat
     8221 gggggcgcaa attatcaaaa cctattatgt cgaaaaaggt tttgaacgga ttgttgccgg
     8281 atgtccggta cccattgtta ttgctggcgg taaaaaatta ccggagcgcg aggcgctgga
     8341 aatgtgctgg caggctatcg atcagggcgc ttctggtgtg gatatggggc gtaatatttt
     8401 ccagtctgac catccggtgg cgatgatgaa agccgtacag gcggtggttc accataacga
     8461 aacggctgat cgggcatatg aactctatct gagtgaaaaa cagtaactgc ggatttaagg
     8521 agaagaatta tgcacgtcac actggttgaa attaacgttc atgaagacaa ggttgacgag
     8581 tttatcgaag tttttcgcca gaaccacctg ggctcggtac aggaagaagg caatttgcgc
     8641 ttcgatgtct tacaggaccc ggaagtgaat tcgcgctttt atatctacga agcctataaa
     8701 gatgaagacg cagtggcatt ccataaaacc acgccccact acaaaacctg tgtcgcgaaa
     8761 ctggaatctt taatgaccgg gccgcgtaaa aaacgtctgt tcaatgggtt gatgccgtga
     8821 ggcgaattta tcaattttat ctacaattgg ggtaacgcgc tgacgggagt aaaaaaatgt
     8881 ctgactggaa cccctcttta tatctgcact ttgccgctga acgatcgcgt ccggcggtgg
     8941 agctgcttgc cagagtgccg ctggagaatg tcgaatatgt tgccgatctt ggctgtggcc
     9001 caggtaacag caccgccctt ctacaacaac gttggcctgc tgccaggata acgggcatcg
     9061 actcgtctcc ggcgatgatt gctgaagcgc gcagtgcttt gccagactgc cagttcgtgg
     9121 aagcagacat ccgcaactgg caaccggtac aggcactcga tctgattttt gctaatgcct
     9181 cactgcaatg gctgcccgac cactacgaat tgtttcctca tctggtttct ttacttaatc
     9241 cgcagggcgt gctggcagta cagatgccag ataactggct ggagccgacc catgtgctca
     9301 tgcgcgaagt tgcctgggaa caaaactacc cggatcgtgg gcgtgagccg ttggctggcg
     9361 ttcatgctta ctacgatatt ttgagcgaag ccggatgtga ggtcgatatc tggcgaacca
     9421 cctactatca ccagatgccg tcacaccagg cgattatcga ttgggtgacc gccactggat
     9481 tacgtccgtg gttacaggat ctgaccgaga gcgaacagca gctttttctt aagcgctacc
     9541 atcagatgct ggaagagcag tatccactgc aagagaacgg acagatactg ctggcatttc
     9601 cgcgtctttt tattgttgcc cgccgtacgg agtaaatatc acgtcagctg gtaatgacga
     9661 tcgggaagaa tttttgctgg aatttcggct tcatcgttca tctgtaacag gtcaatttca
     9721 atagcgttgc agatggcatc cagcggtaaa tcattgtttt cagtaccgaa cggatcttcc
     9781 agttcttccg ccagacaatc cagcgaaata aaagtgtagg aaatcagcac agagataaaa
     9841 ggcgtcatgt aatgcaggtc cacgaccagc gcgaacggca gcatgataca aaacagataa
     9901 acggtacgat gcaaaatcag ggtgtaggca aagggaattg gcgtataggc aatgcgctcg
     9961 catcctgcca ggaccgctga aatatcatta agccgatcgt tgaggctaat aaacaggata
    10021 tctgaaagct gcccattgcg gcgctgaacc gccaaccact cccccattat taacaagata
    10081 cggttagctg gagagttcga agccagtaca ggctgaagat cttcagtctt gagataatga
    10141 gccagcactt ccgcctgtgg ctgtttgcgt aatgtcatgc gtaaacagtg ggcgaaggcg
    10201 atttgcaacc gggcaaactc ccttacactt gccgaatccg gcaatgtcgt ttttacctcg
    10261 cgcagtaacg accgtgaggc aatcatcaac tgcccccaaa gttttcgcgc ttcaacgtaa
    10321 cgggcgtacc cggcattatt acgaaaacca agaaaaatgg cgatggcgac accgagaatg
    10381 ctgaacggtg cgagggtgaa tttgatgccc agatgcgtgt accagggcag catgaaaata
    10441 acagcgatag aaaagagaaa attgagtagt aagcgcgatg atatcttgga taatactgag
    10501 ccgtgccaga caaaaatacg gcgcagccag tgttgttgtg gacgaacaat catggttatc
    10561 ttcaggcgtg aaaaagtcgc cctattaaac gtgattatga tcacattctc aagacgctac
    10621 ttacaaatta cctacaagct tacaacatta acaggcgctg catgtggcac ctgttaatga
    10681 taaaggcaca taggatgttg taactaatat agtgaaataa gaaacccggt cgaaaccggg
    10741 ttcagaagta acggtgttat tagcacaacg gacgtacagc ttcgcgcatc cctttttcaa
    10801 gaatcgcatc caggtcatta gcaacctgct cgaccagacc aggcacttgc gtcaggtcct
    10861 gctcccagtg gtctttttct gccaacacaa tcgctaccag ttcctgggta ccgataacac
    10921 ggtcacgatg ctggctccag agttgctgat aacgttccag ccagtgtgca tcgtcctgta
    10981 ccggatatgt ttctccgtta cgctcaccac gatagaaggc aatcaatgcc gctaatgcga
    11041 aagtaagacg cgccggaagt gtgccttttg ccttctgccc tgccagcagc tgcggcagga
    11101 tgcgagtgcg gaacttggtc ataccgttga gcgcgataga cagcagctga tgcttaatgt
    11161 acgggttacg gaaacgcccg gtgactgcac tggcgaaaga ttccagttca tcacgaggca
    11221 aatccagtac cggaataatt tcttcgtaga tagctttttc aacgaatgcg caaatttcag
    11281 catcgttcat cgcttcacct acggtatcca gccctgcctg gaacgccacc ggcaccagcg
    11341 cggtgtgcgc accgttgagg attgccactt tgcgttcttt gtacggttta atatcgtcaa
    11401 caatcagcac gttgagcgga tatttgtcca gacgcagttc agtcgctaag gatttcggtc
    11461 cctgaatcac aaacaggtaa aagtgttcag cggtgtcgag aaaaccatcg tgataaccca
    11521 gctcttcttc cagtttagcc acttcatcgc gcggataacc ggtaacgata cggtccacca
    11581 gcgtagaaca gaagctgtta gcctgatcca gccattgaat aaatgcttct ggcagtgccc
    11641 actcttgcgc atagcgcagc accagttcac gcaacgcgtc gccattgtag tcgatcaact
    11701 cacacgggat gatgatccaa cctttatcca gcgcaccgtt gaaatggcta aaacgttcga
    11761 acagcagacg ggtcagtttt gccggataac ttaccgctgg cgcgtcatcg aatttatcgc
    11821 ccgcatggta gctgataccc gcttcggtgg tattagagaa aacaaagcgc atttccgggt
    11881 tgtgagccag tttcaggaat tcatcgtatt cactgtagac gctgatttca cgattaaccg
    11941 agcgaatcag acgcgcgtcg ctgaccgctt cccccttctc attcaggcca cggataatgg
    12001 tggtatacag accatcctgc gtgctcagtg acggtgggaa tgaagtttca atcggacgaa
    12061 caacgaccac accagaattc agatcggtgt gctcattcag gagatcgatt tgccagtcaa
    12121 caaaggcgcg caggaagtta ccttcaccaa actgaatgat acgttctgga tactgtgcac
    12181 cgggaaaatc gcgacgattt agtgttttca caatgggttc ccttctgatt agtcatacaa
    12241 cctgtttgaa ttggtacgac aggttagcaa actttaatac gccgaacccc tattttgatc
    12301 aactcctgat gattaatgag cagttttatg agaaaagtgt ggcgcggatc atggtttaat
    12361 cgaggaaata cgccttttcc tggatcataa agtggtagaa cacattgcat tcaaatcgcg
    12421 cggaatgaat aaagatgtca gacaacttcc tcaccgtaac gcatagtgct ggtacggttg
    12481 cgcccatctt tcttcgagcg atacagacac gtatcggctt cgaccatcaa tttattaaaa
    12541 tcatcggtca gcgttcggta cgatgcgcga ccactaccga cgccaatact taccgtgaga
    12601 taaagcgttt tttgttgcca ggtgaatggt tgcagttcaa cgcctttacg gattttttcc
    12661 gccattagca gaccatcgac aggattcacc gacggcactg caacagcaaa ttcttcgccg
    12721 cccatgcgcg ccaccagccc cttatcaccg acaatcttct gaatatgccg ggcaaacacg
    12781 cttaacactt tatcgccaca ttcatgtcca tagttatcgt taatgctttt gaaatagtca
    12841 atatcaagca gcatgaccgt cagatgttgt gtctgtttca gcgatggact tttcagcgcc
    12901 tcataaagac cggaccgtga gtaaacctga gtcagaaaat caaagtcggc tcgcagcgca
    12961 acttgcttca ttagcgaatt gatcgctgcc acgctaaaag aaaccataat tgggcatatc
    13021 gccatcgtgg caataccgag acgtgcggag aacatttccg gaatggagaa cggcgaaccg
    13081 accgagatat caatcaccga atttgccacc agtacgattt ccaccgcacc ggtgacaaag
    13141 gtcaacagac atgttacctg cggcgtatag cgcactgcac accagattaa tgcaggcagc
    13201 gggaacgcca gactccccgc tccgccaatg actaccgagg caataaccga cacaataagc
    13261 gcgatggcgg gcattatctg ttctgcttta aagcgcggca gcactccagg aatagccaac
    13321 gtcagcatac aaggcacgat caacacgccc gttgagaatt gctcactgaa ccagtccgca
    13381 agcaaaggcc agaaatccag actatcaata ctgaccgaac caatcgcccc gacaatagcg
    13441 cataataatg cggcaatcag acagtaatta aatagccgta aggcacttac tggctcatac
    13501 ttatttttcc ccagacgctt atcccgcacg accagtaagg cgacggtaac aataaacatc
    13561 atattggaga aattgataac cagtgaaacc agtccccatt cggtggttat ggcatcataa
    13621 accagcatcg ccacataact gatcgcatag taatgcagtc gattaagcca gacatagcgg
    13681 gcaaacaccc ccgccattac gccattcagc ggccagaaaa gagaaagttc gtgaaccagc
    13741 cgtaactctg caccaataaa ataaacagcg tggttagcgc aaaaatagcg atgctattac
    13801 gtagcagatg accttcctgg aacaaacgga aagttgaaat ggggtgtaca tgcattaatt
    13861 atctgtccat acaattgcgg gtggcaatta tctcaacgta ataagtaaat tcacataaag
    13921 gatagcatgt tcgctaacag cggcggcaaa taaaggtacc ctgggaatgc tattttatcg
    13981 ttttccgcaa ttctcatggc tgattcatct gtacaaccag ccagtgatag attaccgcca
    14041 cgatataacg ttgttgttct tcgcttaacg aaacaccctg cggaatgtta tgccacttag
    14101 ccagacaacc gcgacagcag gtcgcggtgg cgtgctgagc gataaacacc ggatgcccgc
    14161 gcatgggggt ttgcttaccg tcattagccg gtaatgccgg agcaagacgt ttagcgacaa
    14221 aatccgccgc atgttgttcg atgactggcg cgcctttctc caggcaatac tgacgctctt
    14281 tcatgcccag acgaaagcga gagcgaaatt ttgaacggga taaacgcgcg aagagtggat
    14341 cgagagactg cattaataaa ccgaacgccc taactgtttc gtcaattgtt caaggacggc
    14401 gatacccgca agcgagttac ctgcatcatc cagttccgga ctccagactg cgatggccat
    14461 ttcatgcgga acaatcgcca caataccgcc accaacgcca gatttcgccg gtagtcccac
    14521 ccgccaggca aactcccccg cgttctggta cataccactg gtcgccatca gcgcgttaat
    14581 ttgccgcgcc tgcattggcg tcaccacagg ttcatcaata tgaatagctt tcccctgatt
    14641 agccagaaag acaaacgtcc gggccagctc tacacagctc attttcagag cgcagtaatg
    14701 aaagtagttt tgcagaacgg ttgtcacgtc atgatggaaa ttgccaaacg acttcatcag
    14761 ccaggcgata gccgcatttc gcgcggaatg ttcaaattcg gaacgcgcta ccaccgtatc
    14821 gtatgaaata tcagacacac cgcttaagcc gcgcacgact tccagcatac gttgccgtgg
    14881 tgcgcttagt cgcccttgca acatatcgca gaccaccagc gcaccggcat taatgaacgg
    14941 attacgcgga ataccctgtt ccatttccag ttgcactaaa gaattgaacg gtgatccaga
    15001 cggatctttg ccgacgcgtt gccagatttc ctcttcggag taatgacgca tggcgacaac
    15061 gagactcagc actttggaaa tagactgaat ggaaaaacgt tcttgcgcgt ctccggcctg
    15121 aaaaagctgt ccgtcaacgg tacagatagc aatccccaat cgggaaccgt ctactgtagc
    15181 cagcgccgga atataatccg cgactttacc ctgaccaatg agcggccgca cttgccgcaa
    15241 gatgttttct aaaattgcat tatccatggc gactgccact ttctactcct ggaccgcagg
    15301 tctgaaaaga cctgcgagta tatcagagct gaatatgtcg cgtcagatcc ggtctttcca
    15361 caccgtctgg atattacaga attcgtgtaa gccgaaatgg gaaagctcac gaccaaagcc
    15421 actctttttc acgccaccaa aggccactcg cgcgtcgctg gcacaataac cattgataaa
    15481 caccccaccg cattccagac gtgccgccat ctgtctggcc tgtgtttcgt cggtggtaaa
    15541 aatggtcgct gaaaggccga actcactatc attagccagt tccagcgcat gttctgcatc
    15601 tttcgcaacg gtgattgccg caacagggcc aaacatttct tcccgaaacg cggtcatttc
    15661 tggggtaaca ttcgccagaa ccgttggcgg atagtagtta cctgccccag ccatcttttc
    15721 cccgcccagt aacaaacgcg caccctgcgc cagggttttc tccacctgat gatgcagctc
    15781 atcacgtaaa tcaaaacgag ccattggtcc gagagcgttc tcttcatcac ggggatcgcc
    15841 cattttcaag gctgccgcag ctgccacaaa acgttcggta aatgcagaag caattccctc
    15901 ttcgataata aagcgttttg ccgctgcaca aacctgtccg gtattctgat aacgtccggc
    15961 taccgccgct ttaaccgcca gttccagatc ggcatcgtta agcacaataa atggatccga
    16021 accgcccagt tccagtacgc attttttcag tgccgctcca gcctgtgcgc caatagccgc
    16081 tcccgcacga acacttccgg tcaccgtgac agcagcaatg cgcgaatctt taatcatttg
    16141 actgacaccg tcgttgtcgg cattcagcca gccatatacg ccttgcggga ttcccgcatc
    16201 tttaaacacc tgggcaatga gctgagcaca gcccatcaca ttcggcgcat gtttaagtaa
    16261 gtagccgtta cctgcaagaa tgatgggaac cgcgccacgc atcacctgcc ataacggaaa
    16321 gttccacggc ataatcgcca gaatcgtccc caacggtcga tactcaataa ctgcctgctg
    16381 attttccacc agcgtaggtt ccgccttcag cattgccgga ccatgttctg cataccagtc
    16441 acacaaattc gccgatttcg ccacttcagc gcgcgcctga ttgattggtt tgcccatttc
    16501 acgggtgatc atttgcgcca tttcttcgct acgggcgcgc agagccttac cgataccacg
    16561 cagtttttca gcacgataat ctatatttgt ctcgcgccag tcgcgaaagc ctgctgccgc
    16621 cagctgaagt gcgttttcga tatcgttagc gccagcccac ggcagcacag aaagttgttc
    16681 acccgtggca ggatttatcg aaattgcatg agttgccgga gtaatggtca tcggggtatc
    16741 tcctttatga gtcatggtat gaagatacgc agatttactc ttgctttaaa atgaataata
    16801 ttaagccact tattcacgaa tcgagaatat tatggatctg acccaactgg agatgttcaa
    16861 cgcggttgcc gaagctggca gcataaccca ggcagcagca atagtgcatc gcgtgccgtc
    16921 gaatctcact acccgtctac gccagctgga aacagaactg ggggtagatc tgtttattcg
    16981 cgagaatcag cgtttacgtc tctctcctgc cgggcataac tttttacgct atagccaaca
    17041 aattctcgcg ttagtggatg aagcgcggag cgttgtcgct ggcgatgagc cgcaaggttt
    17101 attttctctt ggttcgctgg aaagcaccgc agcagtgcgc attccggcca cgctggcgga
    17161 attcaaccat cgttatccca aaattcagtt ttcactttcc accggccctt ccggcactat
    17221 gctggaagga gtgctggagg gaaaactgaa tgcggcattt attgatgggc ccattaacca
    17281 tactgccatt gacgggatac cggtataccg cgaggagctg atgatcgtca cgccacaagg
    17341 tcatgcgcca gtaatccgcg ccagtcaggt taacggcagc aacatttatg ccttccgcgc
    17401 caattgttcg tatcgtcgcc acttcgagag ctggtttcat gctgacggtg ccgctccggg
    17461 aactattcac gagatggagt cgtatcacgg gatgttggcc tgtgtggtcg caggagcagg
    17521 cattgcgctt attccacgct ctatgctgga aagtatgccg gggcatcacc aggttgaagc
    17581 gtggccgtta gctgagcaat ggcgttggtt aacaacctgg ctggtctggc gtcgtggtgc
    17641 gaaaacgcgt ccgcttgagg catttattca actactggat gtgcctgact cggcaaaaca
    17701 gggttaccaa tgagctattt ttgatagttc ttgcagctca aatgccctat atagtgatgt
    17761 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagtaattac
    17821 atatctcttc ctgatgattt tcccgtacgt aatattgcac ctcaggtaaa agaagtttta
    17881 aaggatttta ttgatgcact cagtaccata atatgtgatg aagagt
//
LOCUS       DAFNBJ010000099        17632 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000099 DAFNBJ010000000
VERSION     DAFNBJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.099"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            85..1224
                     /gene="arnB"
                     /locus_tag="L2X49_003176"
     CDS             85..1224
                     /gene="arnB"
                     /locus_tag="L2X49_003176"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBN4798211.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKEKGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1228..2196
                     /gene="arnC"
                     /locus_tag="L2X49_003177"
     CDS             1228..2196
                     /gene="arnC"
                     /locus_tag="L2X49_003177"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBN4798212.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELISRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIADAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2196..4178
                     /gene="arnA"
                     /locus_tag="L2X49_003178"
     CDS             2196..4178
                     /gene="arnA"
                     /locus_tag="L2X49_003178"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBN4798213.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYSS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQASDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLCEDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIC
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4175..5065
                     /locus_tag="L2X49_003179"
     CDS             4175..5065
                     /locus_tag="L2X49_003179"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBN4798214.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQLVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIARDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWVGCQQIAGSR"
     gene            5065..6717
                     /gene="arnT"
                     /locus_tag="L2X49_003180"
     CDS             5065..6717
                     /gene="arnT"
                     /locus_tag="L2X49_003180"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBN4798215.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPNFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6714..7049
                     /gene="arnE"
                     /locus_tag="L2X49_003181"
     CDS             6714..7049
                     /gene="arnE"
                     /locus_tag="L2X49_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBN4798216.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7049..7435
                     /gene="arnF"
                     /locus_tag="L2X49_003182"
     CDS             7049..7435
                     /gene="arnF"
                     /locus_tag="L2X49_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBN4798217.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7429..7695)
                     /gene="pmrD"
                     /locus_tag="L2X49_003183"
     CDS             complement(7429..7695)
                     /gene="pmrD"
                     /locus_tag="L2X49_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBN4798218.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7805..9160)
                     /gene="menE"
                     /locus_tag="L2X49_003184"
     CDS             complement(7805..9160)
                     /gene="menE"
                     /locus_tag="L2X49_003184"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBN4798219.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASEF
                     AVQGVVEGCGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENSFPALTSLHIQLVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHSAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9157..10119)
                     /gene="menC"
                     /locus_tag="L2X49_003185"
     CDS             complement(9157..10119)
                     /gene="menC"
                     /locus_tag="L2X49_003185"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBN4798220.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQLPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10119..10976)
                     /gene="menB"
                     /locus_tag="L2X49_003186"
     CDS             complement(10119..10976)
                     /gene="menB"
                     /locus_tag="L2X49_003186"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBN4798221.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10991..11749)
                     /gene="menH"
                     /locus_tag="L2X49_003187"
     CDS             complement(10991..11749)
                     /gene="menH"
                     /locus_tag="L2X49_003187"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBN4798222.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLTG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11746..13416)
                     /gene="menD"
                     /locus_tag="L2X49_003188"
     CDS             complement(11746..13416)
                     /gene="menD"
                     /locus_tag="L2X49_003188"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBN4798223.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13505..14800)
                     /gene="menF"
                     /locus_tag="L2X49_003189"
     CDS             complement(13505..14800)
                     /gene="menF"
                     /locus_tag="L2X49_003189"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBN4798224.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14879..15184)
                     /gene="elaB"
                     /locus_tag="L2X49_003190"
     CDS             complement(14879..15184)
                     /gene="elaB"
                     /locus_tag="L2X49_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBN4798225.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDTADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15239..15700)
                     /locus_tag="L2X49_003191"
     CDS             complement(15239..15700)
                     /locus_tag="L2X49_003191"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN4798226.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15765..16682
                     /gene="rbn"
                     /locus_tag="L2X49_003192"
     CDS             15765..16682
                     /gene="rbn"
                     /locus_tag="L2X49_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBN4798227.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16870..17628
                     /locus_tag="L2X49_003193"
     CDS             16870..17628
                     /locus_tag="L2X49_003193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798228.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFHINTGGHWILVSLQKIVNKKIINYK"
BASE COUNT         4176 a         4734 c         4424 g         4298 t
ORIGIN      
        1 atttcgctcc aaatggcaaa atatacacaa cactctttat agcaaatata agtggacagg
       61 tattcaatgg cggaaggaaa agcaatgtca gaatttttgc ctttttcgcg accagcaatg
      121 ggcgtggagg aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt
      181 ccgaagaatc aggcgcttga gcaagccttt tgccagttga cgggaaatca gcatgccatc
      241 gcggtcagtt cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag
      301 ggcgatgaag tgattacgcc ttccctgact tgggtttcaa ccctcaatat gatttccttg
      361 ttgggtgcaa cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa
      421 gctatcgagt cagccattac gccacgcact aaagccatca ttccggtgca ttatgccggt
      481 gcgccagcag acattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc
      541 gaagatgctg cccatgccgt cggtacgtat tacaaagggc gacatattgg cgcaaaaggt
      601 accgctattt tttcgtttca tgccatcaaa aatattacct gtgcggaagg tggcctgatt
      661 gttactgata atgaaaatct tgcccgccag ctacggatgc tgaaatttca cggtctgggt
      721 gtcgatgcct atgacagaca aacctggggt cgtgcaccgc aggctgaagt attaacgccg
      781 ggctataagt acaatctgac cgatattaac gccgcgattg ccctgacaca gttagcaaaa
      841 ttagagcacc tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca
      901 gctctcccct ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg
      961 tttattattc gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg
     1021 ttaaaagaaa aagggattgg taccgggtta catttccgcg ccgctcacac acaaaaatat
     1081 tatcgcgagc gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc
     1141 tgttcgttgc cgctgttccc ggatatgact accgccgatg ccgaccgcgt catcacagcc
     1201 cttcagcaac tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg
     1261 gtggttattc ccgtttataa cgagcaggaa agcttaccgg aattaatcag ccgcaccacc
     1321 gcagcctgtg aatcactggg gaaagagtat gagatcctgc tgattgatga cggcagtagc
     1381 gataattccg cgcatatgct ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct
     1441 attttgctta accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt
     1501 actggcgact taattattac ccttgatgct gatctccaga atccgccaga agaaatcccc
     1561 cgcctggtgg caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc
     1621 caggacagct ggtttcgtaa aaccgcttcg aagatgatta accggcttat tcagcgcacc
     1681 actggcaaag cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgcc
     1741 gatgcgatgt tgcactgcca tgaacgcagc acctttatcc cgattctggc gaatatcttc
     1801 gcccgccgtg ccattgaaat tccagtacat catgccgagc gtgagtttgg tgaatccaaa
     1861 tacagtttta tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg
     1921 ccgctacgta tgctgagtct gctcggcagc attattgcga ttggaggttt tagcattgcg
     1981 gtgctgctgg tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt
     2041 atgctatttg ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattactc
     2101 ggtgaatata tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag
     2161 caagttatcc gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc
     2221 acgatatggg atgcctcggt attgaagctc tgctggctgc cggttacgaa attagcgcca
     2281 tttttaccca taccgataat cccggtgaaa aagcctttta tagttccgta gctcgtctgg
     2341 cggcggaaag aggcattcca gtttatgcgc cggataacgt taatcatccg ctgtgggtgg
     2401 aacgcattgc ccaactgtca ccagaggtga ttttctcttt ttattatcgc catcttattt
     2461 gcgacgagat tttgcagctc gctcccgcag gtgcatttaa tctgcacggt tcgctgttac
     2521 caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt gaacggtgaa acggaaacgg
     2581 gcgttacatt gcaccgaatg gtaaaacgtg ccgatgctgg ggccattgtg gcgcaactgc
     2641 gcgttgccat tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg
     2701 cgcgccagct actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg
     2761 cccagcgcga aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttccttg
     2821 aatggcataa accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc
     2881 cgggtgcctt cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc
     2941 ctcatgccag caaagcacag ccggggagcg tgatttctgt tgcgccgctg ctgattgcct
     3001 gtggcgatgg tgcgctggaa atcgtcaccg gacaggcgag cgacggcatt actatgcagg
     3061 gctcgcaatt agcgcaaaca ttgggcttgg tgcaaggttc acgcttgaat agccagcctg
     3121 cctgcaccgc ccgacgccgt acccgggtac tcatcctcgg ggtaaatggc tttattggca
     3181 accatctgac agaacgcctg ctgtgcgaag atcattatga agtttacggt ctggatattg
     3241 gcagcgatgc gataagccgt tttctgaatc atccgcattt tcactttgtc gaaggcgata
     3301 tcagtattca ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc
     3361 tggtggcgat agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg
     3421 attttgaaga gaatctgcgc attatccgct actgcgtgaa gtaccgtaag cgaatcatct
     3481 tcccgtcaac ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt
     3541 ctaatttaat cgtcggcccg gtgaataaac cacgctggat ttattcggta tcaaaacaat
     3601 tacttgatcg ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc
     3661 gcccgtttaa ctggatggga ccacgactgg ataaccttaa tgcggcacga atcggcagct
     3721 cccgcgcgat tacgcaactc attctcaatc ttgtagaagg ttcaccgatt aagctgattg
     3781 atggcggaaa acaaaaacgc tgctttactg atatccggga tggtatcgag gcgttatacc
     3841 gcattatcga aaatgcggga aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg
     3901 agaacgaagc gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc
     3961 cgctgcgcca tcacttcccg ccgtttgcag gctttcgcgt tgtcgaaagt agcagctact
     4021 acggcaaagg atatcaggat gtagagcatc gtaaaccgag catctgcaat gcccgccgct
     4081 gcctggactg ggaacccaaa attgatatgc aggaaaccat cgacgaaact ctggatttct
     4141 tcctgcgcac cgttgatctt acggataaac catcatgacc aaagtcggct tacgcattga
     4201 tgtcgatacc tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa
     4261 gcataatatt caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct
     4321 ctggcgactg gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct
     4381 ttatggctgg gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc
     4441 caatgccgat atcattcgtg aagcggctaa acatcacgaa gtcggcctgc acgcctggga
     4501 tcaccatgcc tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat
     4561 tgcacgcggc cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc
     4621 tgcgggctgg cgtgccgacc agctggtgat cgaagcaaaa gaagcgttcc atttgcgcta
     4681 caacagcgat tgtcgtgggg ccatgccgtt ccgtccactg ctcgaatcag gaaaccctgg
     4741 cactgcgcaa attccggtga ccttacccac ctgggatgaa gtgattgctc gggatgtgaa
     4801 agcagaagat tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt
     4861 ttataccatt catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct
     4921 caaacgcgca gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagagac
     4981 gttgccgctc ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct gggtgggttg
     5041 ccaacaaatt gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg cctcttcgcg
     5101 tttattgcct gctattacct gttaccgatc agcacgcgtc tgctatggca acccgatgaa
     5161 acgcgttatg cggaaatcag tcgggaaatg ctggcatccg gcgactggat tgtgccccat
     5221 ctgttagggc tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggt
     5281 caatggctat ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg
     5341 ttaactgccg cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct
     5401 ctactcgcca cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc
     5461 gtgctcgatc cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca
     5521 atgcaggcac agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc
     5581 ggcatggggg tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg
     5641 ccatgggtag cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt
     5701 atcagttgcg tactgacggt tctcccttgg ggactggcga tagcgcagcg ggagcctaac
     5761 ttctggcact attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa
     5821 catagagctc cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg
     5881 ggattactcc ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc
     5941 tatttgttga gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggtaaattg
     6001 cccacctata ttctttcctg ctttgcatct ctggcaatgc tgatggcgca ttacgctttg
     6061 ctggcagcaa aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt
     6121 ggcgtcactg gcattattgc cacatttgtg gtctccccgt ggggaccaat gaacacgccg
     6181 gtgtggcaaa ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg
     6241 gcatttttcg gctggtacac cttaacaaac gtcgaaaaga cctggccttt tgccgcgctt
     6301 tgcccgctgg ggctggcgtt gctggtagga ttttcaattc ctgacagagt tatggaagga
     6361 aaacatccgc aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt
     6421 cttactgata gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc
     6481 atcatgtatc gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg
     6541 agatttgtca gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt
     6601 attactctcg tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc
     6661 gcagatgcca tcgatcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct
     6721 ggctaacatt agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg
     6781 caacctgctt tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg
     6841 cgctggcttg tcttggtctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg
     6901 taggcattgc ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa
     6961 aactgtggca cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggcg
     7021 gcattgtgat cctcgggagt acggtgtaat gggcctgatg tggggattat tcagcgtcat
     7081 tattgcctca gttgcgcaat taagcctggg ttttgcggcg agtcatctgc cgccgatgac
     7141 gcacttatgg gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt
     7201 actcgggctg ctgggttatc tgctttccgt tttttgttgg tataaaacgc ttcataaact
     7261 tgcgctaagc aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc
     7321 aatggtactt cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg
     7381 tattatgagc gggttgatgc tgattttctt gcccacgaca aaacaacgtt actgagtttt
     7441 ccctgccact ttacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac
     7501 tttcaccgta tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag
     7561 taaatccccc actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc
     7621 gccagcatcg cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt
     7681 gaccagccat tccattgcat tatcctgttt gctaagagtt ttcacatcaa tttacagatt
     7741 aacagataaa ctctttatca acggcaatgg aatggcagaa atgagaagtg aaacggaggg
     7801 ttaattattg ctgacgttgc acccactctt ttagcgcctg acgtgaaatt ttaataccgc
     7861 cgtttttcag ctccggcggc agagttagcc agcgcaccgg ttgttgaaaa cgggccagct
     7921 tatctttcac ccattcacta agatcaacgc tctcgtggtc atactccatc accgccaccg
     7981 gtcgatgacc aaactccttg tcggcaacgg ggacgataaa cacctgcaaa accgcagaat
     8041 gtgcagcaat tacgcgctcg acttcttccg gctgaatacc ctctccgcca ctgaagaata
     8101 gattgtctaa acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg
     8161 tagcgtacca gccttcgtca ttaaccagtg aaaccagttg cccgttacgc cagtaacctt
     8221 ctgccatact ggcagcccgc agccacactt cattattaac gattttcact tcccgacccg
     8281 gcagcggcga accaacgtct gccaggccgt cggcttcttt cgcacacacc gtggaggcaa
     8341 actcggtcag accatagccg caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca
     8401 actcgaccgg gatagccgcg ccgccaagta acaccgcttt cagggaaacg gaactacggt
     8461 taaccagcaa acgccagagt tgtgttggca ccagtgaagc gtgagtacag cctgccagca
     8521 tttgctccaa tggctgttta tcacgtaccg tcatccgcgc accagcgtat aaccagcgcc
     8581 acataattcc ctgaccggag acgtgaaaca gcggtaaaga gagcaaccaa tcatcgtgat
     8641 cgccaaacgg aatcagcgat aacacacctt gcgcactggc aagatgggcc tgataagtat
     8701 ggacagcggc tttcggcaaa ccggtagaac ccgaggtcaa cgtcattgag cacagacgcg
     8761 tcggctgcca cgtagcggca tgtgcgcctt caaccagctg aatgtgcagc gacgttaatg
     8821 ccggaaacga gttttcccca tccggcacca gcgcaaattg cagcgtcaga ttgggcagca
     8881 attcttcaag caacggttgc ggcagctgag ggttcacggg caacacccgc gccccgcatt
     8941 gcagtaacgc cagccaggcg agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc
     9001 cgcagccctc aaccacaccc tgcaccgcaa attcggaggc taattcatcg acgcgagcac
     9061 aaagctcgcg ccagttgagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca
     9121 cttgccgcca gtgacgccac ggccagtcag agaagatcat aacaaccgct ccagtgcatc
     9181 aacttccacg acaggcagcg tgctacccgg ccagcgacga acctgctgcg cctgcatcag
     9241 atccagtgtg tctagccccg gaatggtgtc tggcgttaac caggcggcaa tccgcgccag
     9301 ttgcgttaag cctaagctcg attcaatgga agaactgatc accgccgtca gccccagcgc
     9361 gtgcgccgcc tgtacctgct cgcgtacttt ttccagactg cccgtgagcg ttggtttgat
     9421 aactaccgcg cgcacgccct cttcagccac aaaggcaaaa tccggctcgc gcagactttc
     9481 atcccaggca atggcaatgc cggtttcacg ggcaaacgca cgcgaatcat cgcgggtttt
     9541 gcacggctct tcgagaaacg cgatgcggtc gcgataatcc gggttaacgt atttggcaaa
     9601 ctgctgacct ttcagcggtg tccaggcgcg atttgcgtca agacgcaaat gcagatccgg
     9661 aattgcctcc agcaacagat taaccaccat gccgtcgcgc actgcttcgt acaatcccac
     9721 tttgaccttc gccactttct cgcctggcat atctgcaagt ttgaggatca gatcgtccgg
     9781 atcgccatta cacagtggtg ccgcacggta attggccgcc tgcgggagcg tatctgccag
     9841 ctccgccaat gcacagctta cgccaaaggc gacggaaggc agctgcggta attcgcaatc
     9901 gcctgccagc cagttattta cccaggcaag cagcacactt tgcgcctctt cccaggtttc
     9961 ctgactgaag cccggcagtg gggagatctc cccccaccct tcgcgctcgc cttcacgcag
    10021 gcaaacatac agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc
    10081 catggggatc tgccagcggt atacctgcgc gctacgcatt acggattccg tttaaatttg
    10141 ctgaaatcag gctgacgttt ctggttgaaa gcgttgcgac cttcctgacc ttcttccgtc
    10201 atgtagaaca gcatggtggc gttgcccgcc agctcctgca gccccgcctg cccgtcacag
    10261 tcggcgttca gcgcagcttt caggcagcgc agcgccatcg ggctgttttg cagcatttcg
    10321 cggcaccaac ggacggtttc tttttccaga tccgccagcg gtaccacggt gttgacaagg
    10381 cccatatcca gcgcctgttt tgcgtcgtac tgacggcaca ggaaccagat ttctcgcgct
    10441 tttttctgcc cgacgatacg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg
    10501 actttcgggc cagtctgacc gaagatggca ttatctgctg cgatagtcag gtcgcacatc
    10561 atgtgcagaa cgtgaccacc gccgatggag tagccagcca ccatcgcgac aaccggtttc
    10621 ggacaggtac ggatctgacg ctggaagtcc agcacgttca agtgatgtac gccggaatca
    10681 tctttatagc cgccgtaatc accacgcact ttctggtcac caccggagca gaacgcttta
    10741 tcgcctgcgc cagtcagaat gatcacgccg atattgtcgt cataacgcgc atctgccagt
    10801 gcctggatca tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta
    10861 atggtgattt ttgcgatacc gtcggtggat ttttcataac gaatgtcctc gaaaccttcg
    10921 gagcagtcgt gccattcaac cggtgcgtaa agcattgctt catcaggata aatcatattg
    10981 tgtcctttag tcagaaacgc aagatctgcg ccagacttgc gattacgcca gcgggatttt
    11041 cccgatgcgc gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg
    11101 ccagggcgcg gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg
    11161 cgctaaggtt agcacgtaaa tcaggctgga cggcgagaga agtcgcctcc agcatggcgg
    11221 caagggttgc gccattattg ttgctgcgca gcaccaccag ctcccggcgt tgatcgtcat
    11281 tgagcgaggc aaaaacaggc tgttgatacc agtcggcaaa taccgccgtt aacggttctg
    11341 tgcaaaaacg ctgcgcccat tggcgatcgg aacgctgacg ttccgcacgt tgttcagcat
    11401 tttgcagccc cggatgcccg ccttcgacaa taaccccaca aagccccgtc agcccctggc
    11461 aagccgccat catcgccacc cgtccgccaa gcgagtaccc caccagccag aagtcaagga
    11521 tgttgtaact aaccaaggtt ttacgcagta agtcggtgac atcatcaaat ccatcgacgc
    11581 taatcgccgc cgaaccacca tgacctggga gatcaacata caaccgtgag tagtcggcaa
    11641 acgcctcgcc cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca
    11701 gccagggtaa acctggtttt ccgtgttttg cctgcgcgtg caggatcata aatggcttac
    11761 ctgcgccaga agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat
    11821 caccgtggtg gttggcgtgc gccaggcgtc ggcaaatgcc gtttcaagtt cctgccagtt
    11881 ttgcggacga tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg
    11941 cggcatcaga tagaaacgct cacgctcgct ttgcggcgtt ggcaacagcg agaaaatttg
    12001 cccgccgttg ttgttcacca caattaatac cagcggcgca gaaacctgac gcaataacgc
    12061 cagcgcgttg agatcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc
    12121 gcttgcccgc tgaacgcctg cggcggtcga aagcagtccg tcgataccgc tggcccctcg
    12181 gttgctgtac accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac
    12241 caggctgtta ccaacaaaca attgcccctg ttcaggcaga tagtcgcaga tgcgatgcgc
    12301 cagttgcgct tcgccaaacg catcacggcg ggcaataacc gcctgcattg cctgttccgc
    12361 caggcgcggg atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca
    12421 gtcggcaata ttggcaatta agcgacgtcc gcgatggtgt gccggatcaa gtcgcccttc
    12481 aatgtcatca acaatccagt actcttctgg ttcacagctt gcctgccatt gcaggagccg
    12541 tttccccgtc aggctgcttc ccagttgcac cacaatttgc gcctgctgca gctcgctggt
    12601 cgctttggca ttgcctaacc aaagatcggc acacggcagc ggctgcccgg tttgtgacag
    12661 cacatcgcca atcagcggcc agccaagagt ttgcgcccac agggcaactt ttttgccctc
    12721 ttccgcactc atgcgcccgg caaccaccac gccgcgcttt tgtcgccaga agaaccagtc
    12781 gcgctgtttt tcactttcca gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca
    12841 ccagtcaccc agacgctgtt gccagctaag cccggtatcg tccatttcgc catacagcgg
    12901 ttcagcaaac gggcagttga tatggactcc ccccgcatga agcgtaccga gagcgtggtc
    12961 gatggtagaa accagccaac gtgcggggat atcctgggtc gggcgcggca atgaaatact
    13021 gtgcgtgggg tgagaggcga acattcccgg ctggcgaatt gcctgattcg cgccgcagtc
    13081 aattagctcc ggcgggcgat cggcggttaa gagaatcagt ttttctccgg ttaacccggc
    13141 ttcaatcagt gccggataga gatttgccac cgccgtgccg gaggtcacaa tcaccgccac
    13201 cggctgcttg ctgactttcg ccagccccag cgccagatgc cccaacccac gctcatcgaa
    13261 atgggtgtgg tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg
    13321 cgagcctggg gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac
    13381 cgccgcccag cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc
    13441 ggctaagtat aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc
    13501 attactattc catttgtaat aaagtacgca gccctgccgc tttgttgtcg atttcctgcc
    13561 actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc agcatataat cgcacgacat
    13621 tgccgctaat ttttgctgag cgcagggaaa cgcagaattc gctttgttgt aatgagagat
    13681 agcccgcaga accggcgtac cattctcggg tgaacggttc gtgacgggcg ataaactgtc
    13741 gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga
    13801 tcacatcatc cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgcactttac
    13861 gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc tgtaatcgtt
    13921 ggcagatatc ttccaccacc agcatgttct cgcgctggtt tttatcatcc gccatcagcc
    13981 actctcctaa ctgctgcgcc tgcttatcat caggattatt tgctactgtt cccgccagcg
    14041 cttcagtacg cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gagccaagaa
    14101 aagcattttc gccatcaaag gccatgtaaa aatggtagca attcagattc agtcgacgac
    14161 tggcagccat catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag
    14221 cgagcaccac tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt tcgattaatt
    14281 gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag
    14341 gcaagggctt gatactcacc agtgtggcga taaattcttt tgcctgaatc gcatcgtgct
    14401 gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc
    14461 attccaggcg gggtaaaagt aaattgccct gcgacgggtc aaaagcattc agcccccaaa
    14521 tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg
    14581 taaaacgggt aatcgcgccc aggacggcag cttcttcatc accattacgt tgttgccagt
    14641 agaattgcgg gtacgtttgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga
    14701 gagggaaagg aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat
    14761 ggcgcagtag attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa
    14821 agcctcacat tatacggggt actacaaaaa aaatgcagta ccccggtgta gggaggtttt
    14881 aacggcgtgc cagcaacagt cctagtacca gcccaacggc cgcgcccaca ccaattcctt
    14941 gccagggttt ttcgtggacg tagtcatcag cacgataaac tgcctgcttc gcccgatagt
    15001 aataactgtc tgaagcctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac
    15061 gcgctttcag ctcaacatat ttctgatcgg cggtatcgcc agaggagcgg agcacctctt
    15121 ccagtgtttc actaagcagc gtcaggtcgt catcgatacg tgtatcacca aactgattag
    15181 acattctcgt tctccatttg cgtaaaacct gtcactaact atagacaagg gttgttgatt
    15241 acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcatagacct
    15301 ccgtcacagg gataaaaccg aaactctggt agaagttttg cagatgcgcc tgcgccccga
    15361 ggtaaacagg cttatcaggc cagtgatgcg tacatgtttc gagtgtttta ctcatcagct
    15421 gctgccccac tttttcgcca cgcaacgctt cactgacaat cacccgacct ataacgaccg
    15481 gctcaagatc atcatcactt ttcagaatcc tcgcatatgc caccagttca tcatttttcc
    15541 aaccgagaat atggcgatta tcccccgtca aatcatcacc atcgatatcc tgataagggc
    15601 aattctgctc gacaacaaac accgcgcaac gcagttgtaa taaggcataa agctgggaaa
    15661 ccgataattc agagtgatgc agatcttgcc attcaatcat aatgctctcc ttttagatgt
    15721 atgccttata ctagccggaa tattttttga aacgtgatga actcatggaa ttaatttttt
    15781 taggtacttc ggccggtgtg ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc
    15841 tgcaacatcc gacccagagc ggtctttggt tgtttgactg cggtgaaggc acccagcatc
    15901 agctactgca taccgccttt aaccctggaa aactggacaa gatttttatc agtcaccttc
    15961 atggcgatca tctttttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta
    16021 tccaaccctt aacgatttat ggtccgcaag gtatccgtga atttgtggaa accgcgctgc
    16081 ggattagcgg ctcatggacc gattatccgc tggaaattgt cgaaattggc gctggcgaaa
    16141 ttctcgatga tggcctgcgc aaagtaaccg cttatccgct ggaacaccca ctggaatgtt
    16201 atggctatcg tattgaagaa catgataaac cgggtgcatt aaatgcccag gcattaaaag
    16261 ctgctggcgt gccgcctggc ccactgtttc aggaattaaa agcgggcaaa acaatcacgc
    16321 tggaagatgg aaggcagatt aacggcgcag attacttagc tgctccagtg ccaggtaaag
    16381 cgctcgctat tttcggcgat accggcccct gcgatgccgc acttgacctg gctaaaggtg
    16441 tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa gccaatagtc
    16501 gcggccatag ctctacacgc caggctgcga cactagcccg tgaggctgga gtcggtaagc
    16561 taatcattac ccacgtcagc tcgcgctatg atgacaaagg ttgtcagcac ctgttacgtg
    16621 aatgcaggtc aattttcccg gcgactgaac tagcgaatga tttcaccgtg tttaacgttt
    16681 aactctgctt attattaaaa acagggcgaa acttgccctg ttatcgcaac ccgcgctgca
    16741 tatatccacc cacacgggca tattgagcga tttttgatat ttattcatct ccttacgcac
    16801 tatattcaga gcaataagtt gcctcttttt atcttcattc agcacaacct ttgcaactaa
    16861 aaggagccca tgatggttac agttgtcagc aattattgtc aattatctca aacccaactc
    16921 agtcagacat ttgcagaaaa atttactgtg accgaggaat tactgcagtc tttaaaaaaa
    16981 acagcgttat ccggagatga agaaagtatt gagttactgc ataatattgc gttaggttat
    17041 gatgaatttg gaaaaaaagc tgaagatatt ctttaccata ttgttagaaa cccaacaaat
    17101 gagaccctat cgattatccg acttattaaa aatgcctgtt taaaattata taatttggca
    17161 cataccgcaa ccaactccca ccttaaacca actggtccgg ataattcaga tgttctcctg
    17221 ttcaaaaagc tattctcccc ttcgaaatta atgacaatta tcggtgatga aattcctctt
    17281 atatctgaaa aacagtcgct ttcaaaggtg cttttaaatg atgagaataa tgaactgagt
    17341 gatgatacaa acttctggga taaaaatcgt caattaacca cagatgaaat agcttgctat
    17401 cttcagaaga tcgccgctaa tgcaaaaaat actcaagtca attatcctac tggtctctac
    17461 gtcccctact ccaccagaac tcacctggaa gacgctctca atgaaaatat taagagcgat
    17521 ccatcatggc cgaatgaagt ccagttattc cacataaata ctggcggaca ctggatatta
    17581 gtttcgctac agaaaatagt aaataaaaaa ataataaact acaaataaaa tg
//
LOCUS       DAFNBJ010000100        17627 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000100 DAFNBJ010000000
VERSION     DAFNBJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.100"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            26..529
                     /locus_tag="L2X49_003194"
     CDS             26..529
                     /locus_tag="L2X49_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBN4798229.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(596..2053)
                     /gene="nuoN"
                     /locus_tag="L2X49_003195"
     CDS             complement(596..2053)
                     /gene="nuoN"
                     /locus_tag="L2X49_003195"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBN4798230.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYVPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2060..3589)
                     /gene="nuoM"
                     /locus_tag="L2X49_003196"
     CDS             complement(2060..3589)
                     /gene="nuoM"
                     /locus_tag="L2X49_003196"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBN4798231.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3753..5594)
                     /gene="nuoL"
                     /locus_tag="L2X49_003197"
     CDS             complement(3753..5594)
                     /gene="nuoL"
                     /locus_tag="L2X49_003197"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBN4798232.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5591..5893)
                     /gene="nuoK"
                     /locus_tag="L2X49_003198"
     CDS             complement(5591..5893)
                     /gene="nuoK"
                     /locus_tag="L2X49_003198"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBN4798233.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5890..6444)
                     /gene="nuoJ"
                     /locus_tag="L2X49_003199"
     CDS             complement(5890..6444)
                     /gene="nuoJ"
                     /locus_tag="L2X49_003199"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBN4798234.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6456..6998)
                     /gene="nuoI"
                     /locus_tag="L2X49_003200"
     CDS             complement(6456..6998)
                     /gene="nuoI"
                     /locus_tag="L2X49_003200"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBN4798235.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7013..7990)
                     /gene="nuoH"
                     /locus_tag="L2X49_003201"
     CDS             complement(7013..7990)
                     /gene="nuoH"
                     /locus_tag="L2X49_003201"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBN4798236.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7987..10713)
                     /gene="nuoG"
                     /locus_tag="L2X49_003202"
     CDS             complement(7987..10713)
                     /gene="nuoG"
                     /locus_tag="L2X49_003202"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBN4798237.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSNKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQEHLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYYSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10766..12103)
                     /gene="nuoF"
                     /locus_tag="L2X49_003203"
     CDS             complement(10766..12103)
                     /gene="nuoF"
                     /locus_tag="L2X49_003203"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBN4798238.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12100..12600)
                     /gene="nuoE"
                     /locus_tag="L2X49_003204"
     CDS             complement(12100..12600)
                     /gene="nuoE"
                     /locus_tag="L2X49_003204"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBN4798239.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12603..14405)
                     /gene="nuoC"
                     /locus_tag="L2X49_003205"
     CDS             complement(12603..14405)
                     /gene="nuoC"
                     /locus_tag="L2X49_003205"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBN4798240.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14499..15161)
                     /gene="nuoB"
                     /locus_tag="L2X49_003206"
     CDS             complement(14499..15161)
                     /gene="nuoB"
                     /locus_tag="L2X49_003206"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBN4798241.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15177..15620)
                     /gene="nuoA"
                     /locus_tag="L2X49_003207"
     CDS             complement(15177..15620)
                     /gene="nuoA"
                     /locus_tag="L2X49_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBN4798242.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16251..17189)
                     /gene="lrhA"
                     /locus_tag="L2X49_003208"
     CDS             complement(16251..17189)
                     /gene="lrhA"
                     /locus_tag="L2X49_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBN4798243.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4386 a         4924 c         4582 g         3735 t
ORIGIN      
        1 atgaaattca atgaaggaaa aataaatggg aatgattggt tattttgcag aaattgactc
       61 agaaaagatt aaccaactgc ttgagtctac tgaaaaacct ttgatggata atatacatga
      121 cacgctttca ggattacgcc gactggatat tgataaacgc tgggattttt tacactttgg
      181 gctgacaggc acctctgctt ttgatcccgc caagaatgat ccgctgagtc gtgccgtgct
      241 tggtgaacac agcctcgaag atggcataga tggcttcctg ggattgacct ggaatcagga
      301 gttagccgcg acaatcgacc gactggaatc actggatcga agtgagctgc gtaaacagtt
      361 ttccattaag cgactcaatg aaatggagat ttatccggga gtgacctttt cagaagagtt
      421 agaggggcaa ctctttgcct cgatcatgct tgatatggaa aagctgatta gcgcgtatcg
      481 acgtatgctg cgacaaggga atcatgcgct gacggttatt gttgggtgat ggtgtcagag
      541 cgccaaaaaa aaccgccgag catgtcggcg gctttctgac ttacaaacta acagattaca
      601 tcagcggcat tgctaaccgc acaatgctaa tcagcggttg tggccataca cccagcacca
      661 gtaccaacag tgcagaaatc agcaccacta taccgcccgc gctgtactgc cagtttgatg
      721 gtgcatcgcg acccggttgt tccggggcgt gaagatacag gctcaccgcc acgcgcaggt
      781 agtagtagag gccgattgcc gaaccgacaa ccacggcacc caccagccac cacaagtgtg
      841 cctggacacc gaccgccagc acgtagaact taccgataaa gcccagcgtc atcgggatac
      901 cggccagaga cagcatcatc accgtcatca ctgccgcgag gatcggacga tgccagaaca
      961 gaccgcggta agagaacagg gaatcagcat ccgggccacg atacgggctg gacatcaggc
     1021 tgaccacgcc gaacgcgccg aggctgctga acagataacc ggccaggtaa acccctaccg
     1081 cttccatcga catctcacca gtttgcagcg caatcagcgc caccagcaga tagccgaggt
     1141 gagagataga tgagtaaccg agcagacgtt tgatgttggt ctggctcagc gccatcaggt
     1201 taccgaagat gatggaggca aaggcgataa tcgccagtac tacgcgaatc gcttcgctgt
     1261 cacccaccgg tacgtagagg aacagacgca tcaccacacc gaagatagcg attttgctcg
     1321 ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct ggcgtccaca
     1381 ggtggaacgg caccagcgag agtttgaagc cgaggccaac aatcatcagt ccgaaacctg
     1441 ccagcaacag cggctcgttg agcataccgt cgccgaggtt tttacccagc gcgacaaacg
     1501 acaggtcgcc agactgtgca tacaccagcg ccataccaaa cagcaggaaa gaagacgctg
     1561 cggcagaaag gatggtgtat ttgatactgg cttccagcga acgtttctgg cggaaagcgt
     1621 aaccgaccag gccaaacagc ggcaaagaga tcagttcaat accgaggaac aaagacgcca
     1681 gatggttggc attcgccagc aggatcccgc ccagcgcggc aattaacacc aacaggtaga
     1741 actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta caggtggcga
     1801 ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg
     1861 gcgtaacgtc catagcgccc gcctggccga caaaccagag cgaaaccagc gccgcgttaa
     1921 gcccaataac cgagagcgta gcgttgagga aatgattgcg tcgccacgca atggagagca
     1981 tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc aggttttgtg
     2041 gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca aaccactgct
     2101 ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag aagcccagca
     2161 gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac atccctggca
     2221 gttcctggct ggcaatctgg cttttcgctt taccgaagta agcgcgatgc aacatcgcca
     2281 gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatc accgggacaa
     2341 cctggaagct gccgaacaga atcataaatt cgccgacgaa gttaccggtg ccaggcatcc
     2401 caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccatttcatc ttgctccaca
     2461 gaccgcccat catgcgcatg tcgcgggtat ggatacgttc ataaagctga ccacaaagaa
     2521 taaacagacc cgccgccgac aagccgtgcg caatcatctg gattaccgcg ccctggtagg
     2581 ccaactggct gccggtgtag atagcaatca gcacgaagcc catgtgggaa accgaggtgt
     2641 aggcgatcag acgtttgata tcggtctggg cgaaagccat ccacgcaccg tagaagatgc
     2701 cgataacccc cagccacata gcgattggcg caaactctgc cgatgcgttc gggaacagcg
     2761 gcagggagaa acgcagcaaa ccgtaagcgg cagttttcag caagatcccc gcgaggtcaa
     2821 cggaaccggc ggtcggagcc tgagagtgcg catccggtag ccagccatgc agcggaacca
     2881 ccggcatttt gactgcgaag gcgatgaaga agcccagcat taacaggtat tccacaccac
     2941 tggacattgg cgtattcagc agctcttcat agttgaaggt ccagacgccg gtcgcattgt
     3001 agtgaacaaa aaccagcgcc aggatggcaa tcaacatcac caggccactc gcctgggtgt
     3061 aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct ttatgccccc
     3121 acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag aagaacagga
     3181 acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc aggttgaggt
     3241 ggaagaagcc ctgatatttt tcgatctctt tccacgaaca tagtaccgcc agcacaccga
     3301 gcagaccggt cagcacgacc atcagcagcg acagcccgtc aatggcgaga tgaatagaga
     3361 taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga attccggcgg
     3421 attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc
     3481 ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc aaagcgttcg gtctgccagc
     3541 acaggaagcc gccaataaag ggaattaata ttagccaggg tagtaacatg gcgatcttta
     3601 ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac agaaaattct
     3661 cttcggactg ggggtagtgt cggatgcacc gcgaaggcgg cttatccgac ctacgttctt
     3721 atatgcccag gggcaatccc acaatcctta actcaacgca gtaccatcaa cagtgccagc
     3781 accacgaccg caccgatgct catggatgcc acataccagc gcagataacc gttctcgctt
     3841 aacagcagac ctttacctgc aaagcgggaa agaaccgccg ggatgttcat cattgagttc
     3901 agcggatcgc gtttcagcaa ccaggcaata cccaggaacg gtttgacgaa cactttgtcg
     3961 tacagccagt caaagcccca ggcgttgtac caccaggtgc ccagcagacg gcccggcgca
     4021 ctgttggcaa tggaggtcac cagagtacgt ttacccagcc acagccaggc tgccagcaga
     4081 atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct gccgtgcgcc
     4141 agctccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc gccaacgaag
     4201 gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt cacggcgtga
     4261 gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt gtagagcgag
     4321 gtcataaacg caccgaccag acctgccacc atcagattga tatgaccatt cgccatcgca
     4381 cccgcgagga tctcatcctt actgaagaag ccagcagtga ccagcggcag cgccgacagt
     4441 gctgcgccgc ctaccaggaa gcagagataa accagcggaa tagatttacg cagaccgccc
     4501 atcttgaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga tgccaggaac
     4561 agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc ccatgcctgc
     4621 acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc gagaacacgt
     4681 ttgatgtctg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac cgccccgaca
     4741 atacccacca gatgcagaac ttccggggtc atcaggaaca ggccgtgggt acgggcgatc
     4801 aggtagacac ccgcggttac catcgttgca gcgtggatca gcgcggagac aggcgtcggg
     4861 ccagccatcg catcggcaag ccatgtctgc aacggcaact gcgcagattt accgaccgca
     4921 ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccgtcagc aaagtgcgct
     4981 ggtgccagtt ccaccatttc gcggaagttc agggtgccca gttcgttgta aagaatgaac
     5041 agtgcgaaag cgaggaacac gtcacccaca cgggtcacga cgaacgcttt cattgccgct
     5101 gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca caggcccacg
     5161 ccttcccagc cgaggtacat cagcagcagg ttgtcggcaa gcaccagaac taccatgctg
     5221 gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc cctcttcacc gcgcatgtac
     5281 caggaggcgt acatgtgaat aaggaaaccc acaccagtga ccaccgagag catggtcagc
     5341 gacaggccgt ccagcaccag attgaaaccg atgttaaagt cgcctaccga catccacgtc
     5401 cacagcggct ggctgtatga ctgctcgccg ttagcgaaga aatcaacgcc gataaaggcg
     5461 gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgaaac gttttcagac
     5521 cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat aatggttaag
     5581 gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc tggcgacgac
     5641 ggtgaagttg cagcagcagc gcaaggccga tactcgcttc tgccgccgcg aggctgatgg
     5701 cgagaatgta catcacctga ccgtcggtct gaccccagta gcttccggcg accacaaagg
     5761 ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc agattgcgac
     5821 ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc agtccatgtt
     5881 gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct
     5941 cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca gacccgcgag
     6001 cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg taatacccac
     6061 tgctttagca ctgattggcg taccgtcgat accctgatca ttaacaccaa ggatggcgta
     6121 aacaatcacc accagcatga tggccgacaa aattgccgga ccaatccaca cctgcggttt
     6181 cagccactgg cgttcctgtt cgatttctga accgcccagg ttgagcatca tcaccacgaa
     6241 cacgaacagc accataatgg cacccgcgta gacgataatt tccagcgcac cggcgaagta
     6301 agcgcccagt gagaagaaca ccccggaaat cgccagcagc gaaataatca ggtacagcag
     6361 tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc
     6421 acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct cttgacgtcg
     6481 ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc catacctgcc
     6541 atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag atcctctttt
     6601 tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt taactgaatc
     6661 gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga gaagttgatg
     6721 cggaaaaatt ccgggtacca gcgaccgtct ttggtttctg ctttttgcag cgagatacag
     6781 ccgaccgggc aggctaccgc gcagaggtta caggctacgc aacgctcttc gccgtccggg
     6841 tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg ctcttccggg
     6901 tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat actacgaacc
     6961 tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc ccttattgcg
     7021 cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc ggcaggcaga
     7081 ttttccagcc gaaggacatt acctggtcat aacgcggacg cggtaacgac gcacgaatca
     7141 aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat ggcggtaaca
     7201 acgggccttg ccagccaccg aagaacagcg tcaccatcaa tgcagagatg gtcacaatcc
     7261 cgatgtactc acccacgaag aacagaccga acttcatacc ggaatattca atgtggtaac
     7321 catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga cataccgcca
     7381 cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gataacgttc cacacatgcg
     7441 cctggctgtt gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc acgcccatca
     7501 gggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc atcgcaccca
     7561 gcaacgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa accgccagac
     7621 ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc caacccggac
     7681 tgactggcac aatcgcaaag gccagcagca gcgaggtaaa ggcaatcatc ggtgccaggg
     7741 taaagatgac gcgatccgag aatttcggga tccagtcttc tttaaagaac attttgatca
     7801 tgtccgcaac cagctggagc gaaccgcccc agccaacacg gttaggtccg taacggttct
     7861 ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccgcag gtgacaacca
     7921 ccagcaggat caccaccgct ttgaggatgg tcagcaggat ctcaatcagt tccggtgata
     7981 tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca gcaccggagc
     8041 aataccggac atacccatcg gcaagcccac ctgccctgcc gtcagccctt cagcgatttc
     8101 aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgtgtgtac ctgcgttcac
     8161 acctaacttc gcggcatccg ctgggttgag tttgatgtac ggctgcggca tacggctctg
     8221 gaagaccgga gcacgctgtg acaattcatc gctgccaaac aggtggtaat acggcgcgat
     8281 acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt aatccagacc
     8341 attttcgctg gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt tgccgcccac
     8401 ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cccggcgccc aggcaaacgg
     8461 cacttgcgaa cggtgcgcag tcggctggtt gttaccttcc atcgagaagg tgaacatggt
     8521 gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca tggcggtacg
     8581 accgctgtaa cggtgcggtt cacgggccag tttctgccca cgaatacgga atgtcgcatc
     8641 cggcgcagca tctttgatac ctgccagttc cgggattttc gccacaacag cgtcaatcac
     8701 atggtcgagc tgcgtccagt ccacttcacg gctcagcagg gtgctgtgca gcgagtgtaa
     8761 ccagcgccag ctttccagca tgacagtttt gctgtagtaa taagcaggat cgtaaacctg
     8821 gaagaaacgt tgggcgcggc cttcgttgtt gatcaccgta ccgtcgcttt cagcaaagct
     8881 ggcagcagaa agtaccagat gggcgttttc cataatcgct gtgcgttgat gatcaaccac
     8941 catcaccagc ggcgctttag ccagcgcagc attcacgcgg gtagcggaag cgtgacgatg
     9001 cagatcgttt tccagcacca ccaccgcgtc ggcgcgtccg gtttccagtt cggtcaacgc
     9061 ttcttcaagc gaaccgccac ccataatgcc cagccccatg ctgttgacgg aacgggcaat
     9121 catggtgata ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt tagccgcagc
     9181 ctgaatcacc tctatgctac cggcgttcgt cccggagata atcaacggtt tcttcgcacc
     9241 tgccagtgcc tgcacgatga cgtcgatttt gctttgcagc tcaggttcga taccgtcaac
     9301 cgctggcgca gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg cctgatcttc
     9361 aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgggtgtcat caacgttggt
     9421 aacaaacagc ggatgcttcg cacgttgacc gatgttgagg attgccgcaa tctgccagtc
     9481 agccactttc tgtgctgccg ccatttcgcg cgctttacct ttcacagcct gacgcactgc
     9541 cagcgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta ccgcatcgta
     9601 agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagca ctttcagcgc
     9661 cagttgcaga tgttcctgct caccgtgagc gataccggtg tagaagtttt cttcgcccac
     9721 cagttcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa taccgatcac
     9781 tttattcgac tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg cgttgagggt
     9841 aatgaaatca tcgccacgac gctgtactgg ttgacgcgga cgatctttca ggttgacgta
     9901 accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg
     9961 gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac agccgatgga
    10021 acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt aacgctcgga
    10081 gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg accaggttac cggaaaattc
    10141 gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg caccgtaaac
    10201 gcccagatcc gtaccgtcag cgtaatcttt gtagtaacgc acgcagcggt agcaggcgat
    10261 gcagcggttc atttcgtgag agatgaatgg ccccaaatcc tgattacggt gggtacgttt
    10321 ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct gaagatggca
    10381 gttaccgcct tcttcacata ccggacagtc gtgcgggtgg ttggtcatta accactcgac
    10441 cacactttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga
    10501 agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt tttggtattg
    10561 cttcaccgca cactggcggc aagcaccgac gcttcccagc gccggatgcc agcaaaagta
    10621 aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgctc cgttgacctc
    10681 gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt tctcggtcag
    10741 ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc ggctgaatcc
    10801 cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat tcttcgcgga
    10861 aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca cagaaagttt
    10921 tacccgggcc taagaatcga cacagttgct caagtgtttc gatatcgccc ggctgacctt
    10981 caccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg cggcacggcg
    11041 tacaccagcc gcaggactca cgggcgaaaa actcttccag gttacgcacc agcgacacca
    11101 tgttgatctc atggtcaacc gccatcgcca gcgccgtacc cagacggctt cccgctttac
    11161 cgatactttc gaattccatc ggcaggtcaa ggtgcgcttc ggtcaggaag tcggtgcccg
    11221 cgccgcctgg ctgccaggct ttgaatttca gaccgtcacg cataccaccg gcgtaatctt
    11281 cgaggatctc gcgcgcggta gtaccaaacg gcagttccca cagtcccgga tttttcaccc
    11341 gaccggagaa gcccatcagc ttggtgccag catctttact tttcgagatg ttctgatacc
    11401 actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg ttgttgacac
    11461 aggtcggttt gccccatacg ccggaggttg ccgggaaggg gggtttcgag cgtgggttag
    11521 cacgacgccc ttccagggag ttgattaacg ctgtttcttc cccgcagatg tagcgccctg
    11581 ccccggtatg gacgaacagt tcgaagtcga aacctgttcc cataatgttt ttgccaagca
    11641 agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt tactgccgct tcgatatatt
    11701 cgccacgcag gaagatgtag ccacggtaag ctttcagcgc aaacgcggag atgagcatac
    11761 cttccaccag caggtgcggc agttgctcca tcagcaggcg gtctttatag gtgcctggct
    11821 ccatttcatc ggcgttacac agcaggtaac ggatgttcat ggattcgtct ttcggcatca
    11881 ggctccattt caggccggta gaaaagcccg cgccaccgcg ccctttcaga ccagcgtctt
    11941 ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc gcgccttcgt
    12001 aaccgttttt gctgcgatat tcgtccagcc acactggctg tttgtcatcg cgcagacgcc
    12061 aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc gctccagcag
    12121 ttcagggatc gcttccgggg tcagatgcgc gtgagtatcc tcatcgatca tcatgtttgg
    12181 ccctttatca cagttcccca ggcagcaagt tggcagcagc gtaaagcgac cgtcaaatgt
    12241 cgtttgccct ggtttgatgt ttagcttttt ctcgagcgcc gcctgaatac cctggtaacc
    12301 gttgatatga cagaccacgc tgtcgcaata acggatcaca tggcggccaa ccggctggcg
    12361 gaagatctgg ctgtagaacg ttgccacacc ttcgacgtcg cttgccggaa tacccagcac
    12421 atcggcgatc gcgtggatcg caccatccgg cacccagcca cgctgcttct gaacgatttt
    12481 cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt gctcaatcgc
    12541 ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat
    12601 aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac aatcaggtca
    12661 gacaccaggc tgccgcggat cgccgccgga atttgctgca aatgcgcata actcggcgtg
    12721 cggatacgag tgcgataact catggtgcta ccgtcgctgg tcaggtagta actgttgatc
    12781 cccttggtcg cctcaatcat ctggaaagat tcattggcag gcatcaccgg cccccacgac
    12841 acttgcagga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc tttcggcggc
    12901 ggcgtggtca gcgggtgatc cgctttgaac gggccttccg gcatgttgtt gaggcactgc
    12961 tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg ggtgtagcag
    13021 tcagaaacac caccaccaac cgggatttca aagtcgaagt tttcatagcc agaataagga
    13081 cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca ggcccgcgcc agtggtgccc
    13141 cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc tttcagaatg
    13201 gtattttgca gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac
    13261 tcacgcagca ggcgatccca gccgcgcggc aggtcgtgcg ctacgccgcc aatacggaac
    13321 cacgccgggt gcatacggaa acccgtgatc gcttccacca gatcgtaaat tttctgacga
    13381 tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt cctgaataaa ggtcgagatg
    13441 tacagcaggt ggctgttgat acggaacagt tcggagagca taacgcgaat gacgttaacg
    13501 cgatccggca cggtgatccc ggccagtttc tctaccgcca gcacgtaagg catttcgtta
    13561 acgcagccgc cgaggtattc aatacggtcg gtatacggaa tgtagctgtg ccaggactgg
    13621 cgctcgccca ttttctccgc accacggtgg tggtagccga tgtctggtac gcagtcgaca
    13681 atctcttcgc cgtcgagttg taaaacgata cggaaagccc cgtgcgccga cgggtggttc
    13741 ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc cgcgcttcat cccccactct
    13801 tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt cagctcaaac
    13861 ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc tttccaggtt
    13921 tgcggcatca tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc aaacagatcc
    13981 caggtttcac gctcatacca gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc
    14041 aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc gatagaaatc
    14101 agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt gcgcagacgt
    14161 tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa
    14221 tcgccaactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc
    14281 gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat caccggatca
    14341 tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc
    14401 accattgcaa atggtccgtg gtgactgtcg gcggagtaag cttcgcgaaa tagatttagg
    14461 aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc
    14521 aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc gctctgattg catattggcg
    14581 cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt gccgatagat
    14641 tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca gcccgggata
    14701 tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa cggaataaat atcgtacata
    14761 ccgccggagt tggcacaggc acccattgag ataacccatt ttggttccag catctggtca
    14821 tacagacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac caccatcagg
    14881 tcagcctgac gcggcgaagc acgcaatact tctgcgccaa aacgcgccac gtcatgcacc
    14941 gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa
    15001 attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa cacgtttttg
    15061 ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg gtaacggtcg
    15121 ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc tcgcggttag
    15181 cgttgacgat tagcgatact gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg
    15241 ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag taacacaaaa
    15301 ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga ggttgaccat
    15361 gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac cagataaaac
    15421 ttggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc aaacggcacg
    15481 tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag catcaggcaa
    15541 cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc gatgacttca
    15601 gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc actgctcttc
    15661 acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt acaatcactg
    15721 tagaaattct tttatacagc taattgatgt ggtcttttac tcctttctat aaccttttgt
    15781 caactttaac aaaagtttct tcacattagt ttacataata tcaacaccat tagcatttaa
    15841 tgccctttca ccccagatcc ttgacgactc caggataatt agatgttgtt gaatcgtgtc
    15901 cgttgtgaag caatggaaaa aatacgggtc tattttgaca ggaatttgtg tcgattcctc
    15961 cccccaaaag agagtatttt cttgatctgt gacacgcttt tgtcattcca taacaaaaac
    16021 gcagcaacaa atttacgtat tttttaacat cattgtagca ggtgattttt ttcaggcgat
    16081 tatttgtgcg ttcgggacgt gaatctctgg tgattgaaaa atgaacagtt ttgtacgttc
    16141 tgcactatgc gaggaaggat ttttactaaa aaaaagccgc tggggtttaa aacaccccca
    16201 gcggctcgtt ttttacacta ttgtctcagg aattatctat cgtccgtcga ttactcgata
    16261 tccctttcaa tcaacaatga atcatcccct tccggagcag acattgggct gtattgccac
    16321 ggattatggt agctctccat cgcctgatag atcacctggg ccagctcatt attactggac
    16381 gggtcatagc acagcaaata ttcggtatca ggtagcggcg gtaagccatc tactccactc
    16441 agcacacgca ggtccgggct catcatctca accggccttg ccgtcacgcc aagaccggct
    16501 ttcactgccg cacgaacggc cggaagcgtc gaggcgacat aagccagtcg ccatggaata
    16561 tctgctttat taagcgtcgc cagcaccata tcgcgaaacg gacttggatc atccagcaat
    16621 acaagaggga tcggctcgcc tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt
    16681 ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac gatgggtggt taccatcaaa
    16741 tccacttcct gggattccag catttctgcc atataggcat tacgtttaac gcggacatcc
    16801 agcgctaatt tcggataaac cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg
    16861 gcagattcat ctgaagcacc gatagttaac acgccctgaa gattactgaa cattaatgat
    16921 gagcaggcct catcattaaa acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca
    16981 tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca gttctttccc aacgagttgt
    17041 tccagacgct gcatttgctg acttacggcg gactgagtac gacacacagc ggcagctgcg
    17101 gcagcaaaag tgttcagatc ggcaacagca acaaatgttc tcagcagatc gaggtcgagg
    17161 ttaattatcg gacgatttgc acttatcata tattatcact tactggcggc tcatactgag
    17221 ctggttaatg ctgtgcacac acaaacaagc aattccattt gtaatgtgcc tccctggcag
    17281 tttcatccgg aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc
    17341 acattgttcc gttataatgc ctgaagtaga tcacagaata tatcttcagg gatcgcatat
    17401 ctattaagtt actcactctt ttctatttat gacatgcgcg tgtttgtata aatgtaaatg
    17461 tgagtccttg ttccactctc gtgcagcatc gctggtcata cgcgaacacg tacaaacagc
    17521 aatggtgagg cggcatcaag agcagggatc cgtcatttat gcgagcattt taccccaaaa
    17581 accttttatt tataagggtc attgcgaatt atctgatgca aagttat
//
LOCUS       DAFNBJ010000101        17546 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000101 DAFNBJ010000000
VERSION     DAFNBJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.101"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(31..531)
                     /locus_tag="L2X49_003209"
     CDS             complement(31..531)
                     /locus_tag="L2X49_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBN4798244.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(617..796)
                     /locus_tag="L2X49_003210"
     CDS             complement(617..796)
                     /locus_tag="L2X49_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBN4798245.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(1177..1983)
                     /gene="trpA"
                     /locus_tag="L2X49_003211"
     CDS             complement(1177..1983)
                     /gene="trpA"
                     /locus_tag="L2X49_003211"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBN4798246.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(1983..3176)
                     /gene="trpB"
                     /locus_tag="L2X49_003212"
     CDS             complement(1983..3176)
                     /gene="trpB"
                     /locus_tag="L2X49_003212"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBN4798247.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(3188..4549)
                     /gene="trpCF"
                     /locus_tag="L2X49_003213"
     CDS             complement(3188..4549)
                     /gene="trpCF"
                     /locus_tag="L2X49_003213"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBN4798248.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(4550..6145)
                     /gene="trpD"
                     /locus_tag="L2X49_003214"
     CDS             complement(4550..6145)
                     /gene="trpD"
                     /locus_tag="L2X49_003214"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBN4798249.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(6145..7707)
                     /gene="trpE"
                     /locus_tag="L2X49_003215"
     CDS             complement(6145..7707)
                     /gene="trpE"
                     /locus_tag="L2X49_003215"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBN4798250.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(7728..7821)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(7798..7842)
                     /gene="trpL"
                     /locus_tag="L2X49_003216"
     CDS             complement(7798..7842)
                     /gene="trpL"
                     /locus_tag="L2X49_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBN4798251.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            7980..8861
                     /gene="yciV"
                     /locus_tag="L2X49_003217"
     CDS             7980..8861
                     /gene="yciV"
                     /locus_tag="L2X49_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBN4798252.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            8858..9478
                     /locus_tag="L2X49_003218"
     CDS             8858..9478
                     /locus_tag="L2X49_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBN4798253.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            9579..10451
                     /gene="rluB"
                     /locus_tag="L2X49_003219"
     CDS             9579..10451
                     /gene="rluB"
                     /locus_tag="L2X49_003219"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBN4798254.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(10491..11081)
                     /gene="cobO"
                     /locus_tag="L2X49_003220"
     CDS             complement(10491..11081)
                     /gene="cobO"
                     /locus_tag="L2X49_003220"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBN4798255.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(11078..11836)
                     /locus_tag="L2X49_003221"
     CDS             complement(11078..11836)
                     /locus_tag="L2X49_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBN4798256.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12056..13105
                     /gene="sohB"
                     /locus_tag="L2X49_003222"
     CDS             12056..13105
                     /gene="sohB"
                     /locus_tag="L2X49_003222"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBN4798257.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13141..13392)
                     /locus_tag="L2X49_003223"
     CDS             complement(13141..13392)
                     /locus_tag="L2X49_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBN4798258.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            13772..16369
                     /gene="topA"
                     /locus_tag="L2X49_003224"
     CDS             13772..16369
                     /gene="topA"
                     /locus_tag="L2X49_003224"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBN4798259.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYMVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            16579..>17546
                     /gene="cysB"
                     /locus_tag="L2X49_003225"
     CDS             16579..>17546
                     /gene="cysB"
                     /locus_tag="L2X49_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBN4798260.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLP"
BASE COUNT         4272 a         4396 c         4607 g         4271 t
ORIGIN      
        1 ataaaatgac taaaagttaa agttcatatt tcaggcttta ttttcggctt tcttatttac
       61 gttattaagg gccagatcag tcagtttgat gtcggtggcc ttttcttctt ccagggtctc
      121 tttcagaagc ttcgctgctt tacgataacc taattgttca gccagcgtcg ctaatgtccc
      181 gtaactggca atctcataat gctcgacttt ctgtgctgcg gcaatcagtg cggcatcacg
      241 cacttcgttt ttctcggtac tttcgatgac ctcattagct tcttcaataa gaccttccat
      301 tgccacacat ttcatgcgct taattttcag attcgattct gattccacaa cttgatcaat
      361 acgttcaatc tgtccatgtg tttcctcgag gtgcgcatga aaagcctgac ttaatttttc
      421 gtttgatgtt gctcttgcga gttttgccag tgcccgggtt aattgttttt ctgcgctgta
      481 ggtatctgaa agcaggtgaa taaatacatc ttcaatggtc ttcatattca tatttttctc
      541 cagtgaaatc actgcgagcc atacggcccg cagatgcaat tatctttatc agctgatatt
      601 aataattaac ggtgaatcag gatttgcctg atttattacc accgctttgt tgaccgcctt
      661 ttttacccgc ttcagatgcg cgttgcggat catttttaaa attaccgccg ctatgctgac
      721 cgcctttacg gcctgcgtcg gatgccttct cacggtcttc ggcgaaattt cctgaaccac
      781 cacgatgttc ggccatgtta tttctcccgt tgcgttgcat tgtttcatta atatgagtgt
      841 tgtgtgtcga cactcattaa aattagtcgc taatgagaat tagtcaaatt aagcgcaacg
      901 agaagataga gggaaaatat attttgagga acattctgga tatattaaca attacctgag
      961 gaataagtga cttagagaaa attattgatt ttactggtgt tatgttgcgg gattaatttg
     1021 tttgattata ttactgttgg gcggaaaaat gacgtaagtt gacgttcgac cggggtaagc
     1081 gaaacggtaa aaagataaat attaaatgaa tttaggattt ttccggcttc attaaagaaa
     1141 gttaaaatgc cgccagcgga actggcggct gtgggattaa ctgcgcgtcg ccgctttcat
     1201 cggttgtaca aaaactttca gtgccgccag cattttctct ggctcattaa tatgttgctc
     1261 gatgatttta acaatggccg aaccagaaat cgcgcccgca gctcctgcat caatcgctgc
     1321 ttttacctga tccggggcgg aaataccaaa cccctgcaat ggaggtgcag cgttgtactc
     1381 tttcagcttc gcaaccagat gattgagggg taacgcggcg cggttttctg cgccggtcac
     1441 gcccgctcgt gacagcaaat aggtgtaacc acgaccgtaa gaggctatct ggcgcagcag
     1501 gtcgtcatcg gcatttggcg ggcagatgaa gataggtgcg acattatggc gcaacgcggc
     1561 ctggcggaag ggcgcggact cttcaattgg cacatcggca accagcaccg aatcgacgcc
     1621 gactttctcg cactgggcat aaaactcatc aatgcctttg ttaaacacca gattggcata
     1681 catcaacagg ccaatgggaa tggtcgggtg tttctggcga atcagtgcca gcatttcaaa
     1741 acattgtgcc ggagtcacac ctgccgcaaa ggcgcgcaga gtggcgtttt gaatggtcgg
     1801 gccatccgcc agtgggtcgg agaaggggat acctaactcc agcgcgtcag caccggcttc
     1861 aattagcgta tcgataattt tcaatgactg ctcaatgccc ggatcaccga gcgtgacgaa
     1921 aggaacgaat gcgccttctt tgcgctcctt caactgggca aacagagatt cgtagcgttc
     1981 catcagattt cccctcgtgc tttcaaaata tcgtgaacgg tgaagatgtc tttatcgccg
     2041 cgaccggaaa ggttaaccac cagtagctgc tctttatccg ggttttcgcg catcattttc
     2101 aacgcatggg ccagggcgtg ggaggattcc agcgccggga tgatcccttc gtgcaggcac
     2161 agcgttttga aggcttcaag ggcttcatca tcggtaatag acacgtaatc agcgcgtcca
     2221 gtgctgttaa gatacgcgtg ttgtgggccg acagacggga aatccagtcc ggcggagatg
     2281 gagtaagatt cttcaatctg cccgtcttcg gtttgcatca tcggcgcttt cataccgaaa
     2341 tagatgccca cgcgaccatg ttttaacggt gcgccgtgct cgccagtttc gataccgtga
     2401 ccacctggct ccacaccaat caggccgacg ttggtttcat tgatgaaatc agcaaacatg
     2461 ccgatggcat tcgaaccgcc gccaacacag gcgataacgg catccggcag gcgaccttct
     2521 ctttccagaa tctgcgcttt ggtttcttcg ccaatcatcc gctgaaactc acgcacaatg
     2581 gtcggataag gatgcgggcc agctgcggtg cccagcatat agtgcgcggt ttcgtaacta
     2641 ccggaccagt cgcgcagcgc ctcgttacag gcatctttca gcgtcgcgga accgctatgc
     2701 accgggatca cttccgcacc cattaagcgc atacgaaaaa cgttaggcga ctggcgttct
     2761 acgtctttgg cacccatata aatacggcat ttcaggccga gcagggcgct ggcaagggcc
     2821 gacgccacgc catgctgacc ggcaccggtt tcggcgatga tttcggtttt acccatccgc
     2881 ttcgccagta aagcctgacc gagcacctgg ttagttttat gcgcgccgcc gtgcagcaaa
     2941 tcttcacgct tgagatacag agtggtgttc gtcccggctg taatgttctg gcatttggtc
     3001 agcgcggttg gacgcccggc atagtttttc agcaggtcgt tgaactgagc ctgaaattca
     3061 ggatcttttt gcgcactgac aaaagcttct tccagctggc gcagagcagg catcaggatt
     3121 tgtggcacgt acatgccgcc aaactcacca aaataggggt taagtaatgt tgtcattgtt
     3181 cctttcctta atatgcgcgc agcgtctgga aaaccgaggc caaaagacgt gcgtctttga
     3241 tgcccggttg cgactctaca gcagaattaa aatcaagtcc ggcgcagccg gtttgtgccg
     3301 cttccacgca gttatctgcg cctaagcccc ccgccagcag aacgttgcca agcgattgac
     3361 catttaatag tgaccagtcg aaacgttgcc cgcttccacc ctggccgttg tctaaaacat
     3421 atttatcaac gtgctgaagc tcgcgggcgg gcagggtttc accgacgctt aatgctttcc
     3481 agatggcaac gtgtgctggc agagcttcac gcagcgtatc gatatacagc tgatcttcat
     3541 taccatgcag ttgcactgcc gccagcgata acaccttagc tttgtccacc acatcggcaa
     3601 tatcgtgatt gcggaacacg ccaacatact gcaacggtgc cgcagccatc acttcctgcg
     3661 cctgttcaac gttgacgcaa cgtggtgatg tcgcaacaaa aatcaaccca ccgtaaatcg
     3721 cgcccgcgtc ataagctgct ttagcatctt gcccacgcgt caggccacat actttattct
     3781 cacccagcaa cacccggcgc acggcggcgt gcaaatcgtc atgggccatc aacgccgaac
     3841 caatcagaaa accgttagcg aagtggctta actcgcgcac ctgagcgtaa gtattgatgc
     3901 cggattcgct gattaccgtt acgttgtgcc ccagtttcgg cgcaagctcg cgggtacggt
     3961 tgagatcaat cgacaaatca cgcagatcgc ggttgttgat gccaacgacc tttgccccca
     4021 atgcaatggc acgctccagt tcctcttcat tactgacttc ggtcagcaca cccatctcca
     4081 gactgtgggc gacggcggca agctggcgat attgttcgtc atccagtact gaaagcatta
     4141 ataagcaggc atcagcctgg taatagcgcg ccagatagat ctggtaagga tcgataatga
     4201 agtctttaca taaaatcggc tgcggggcga tttggctgac gatggggagg aaatcaaagc
     4261 tcccctgaaa atatttctca tcagtcagca ccgaaattgc cgaagcgtaa tgtttataaa
     4321 tggcggcaat gcgtgctgga tcgaaatcat cacggatcac gccttttgac ggcgacgctt
     4381 tcttgcactc cagaataaac gccgtgcgcg caccctgtag cgcatcataa aaatgtcgcg
     4441 tgctcggctg aacctcattc tgaaaactgg ccagcggttg ctgctgtttg cgggtttcta
     4501 cccaaatcgc cttgtctgcg acgattttcg ctaaaacggt ttgcatcatt taccctcgtg
     4561 ccgccagcgc ggtaactctg tcgtaagcgg aaccactgcg cagtacctca agaacggttt
     4621 gcgcattggc ttgcagatct tcatggccat gcaggcgcat taacatggcg acgttcgcag
     4681 cgacggctgc ttcatgggcg gcgtcgcctt taccttgtaa caaacgtgtt aaaatgtcac
     4741 ggttttcttc cggtgttccg cctgccagtt gctcctggtg gtagggtgtc aggccaaaat
     4801 cttcagcggt caattgatag ctcttaattt cgccgtcatg cagctcggca acgattgtcg
     4861 gcgcgtgtaa tgaaacttca tccatcccgc cgctgtgcac caccgccgcg cgttgatacc
     4921 ccagcacgcg caaggtttcg gcaatcggca gcaccagttc cggactataa acaccaatta
     4981 acgccagcgg cggatgcgcc gggttaatca atggccccag cacattgaac agggtgcggg
     5041 tttttagttg ctggcgaacc ggcattgcat ggcgaaatcc ggtgtgatat ttcggtgcaa
     5101 agaggaaaca tacgcctaac tcatccagcg cctggcgcga tttatcggcg ttcatatcaa
     5161 gattaatacc gaacgccgcc agcagatcgg acgaaccaga tttactggag acgctacggt
     5221 tgccgtgttt cgccactttc agcccacagg ccgcggcgac aaacgcactg gcggtagaaa
     5281 tattgatact gttgctgccg tcaccgccag taccgacgat atcagcaaac agataatccg
     5341 ggcgcgggaa cggcgctgcg ttttccagta gcgcggttgc tgccccggcg atctcgttcg
     5401 ggtgctcacc gcgaattttc atgctcacca gcgccgccgc cagttgttcc ggcttcagct
     5461 cgccacgcac caccgctgaa aacagctggt ggctttcttg ttggctaagc gtctgcgcct
     5521 gatacagttt ttccagaatc ggttgcagcg tgttggctgg ctctagtttc tgctgcgccc
     5581 aggccagcgt ttgttccagc aggcgagcgc cctgggtggt gagaatggat tccggatgga
     5641 actggaatcc acaaacgcga tccgcatcgt gacgtactgc catcaccatg ccattaaaat
     5701 gggcgttgat ggttaaaccg gccggaatgt tactgccaac cagcgagtga taacgcgcca
     5761 ccggcagcgg gtttgttaat ccggcaaaca tcgcctgacc gtcatgttca atgctcgacg
     5821 ctttaccgtg aagaatttcg cccgcctgac cgacatagcc cccgtaagct tcgacaattg
     5881 cctgatgtcc gaggcaaatg ccaataatgg gcagcttgcc acgcaagcgg gtgaggagtt
     5941 ccggcataca accggcttcg ctcggcacac cggggccagg agaaagcatc agtaccggat
     6001 tgctcatggt cgccaggcgt tcaattaagg tctgcgccgg aatatggttg cggtaaatca
     6061 ccacgttatg cccattgctg cgcaactgat ctgccaggtt gtacgtaaaa gagtcgatat
     6121 tatcgagcag cagtatgtca gccatcagaa agtctcctgt gcatgatgcg cggtggcaat
     6181 agcgcgcagt acagcgcggg ctttattacg ggtttcgtcg gcttccgact gcggaataga
     6241 atcaaggact acgccagcac cggcttgcac ggtggcgata ccgttttcca ccagcgccga
     6301 gcggatcaca atgcaggtgt cgagatcgcc gtgcgcggtg aaataaccta ccgcgccgcc
     6361 gtagctgccg cggcgacgac cttccgcctc ggcaattaac tgcatggcgc gcactttcgg
     6421 cgcaccgctt aacgtcccca tattcataca ggcgcggtaa gcgtgtaggg cgtcgagatc
     6481 gtggcgcagc tcaccgacca cgcgggagac caggtgcatc acgtaagagt aacggtcaac
     6541 tttggtgagg tcggcgacgt agcggctgcc gggggtgcaa atgcgtgcca gatcattacg
     6601 ggcaagatcc accagcatca gatgttcaga aagctcttta tgatcggtac gcatttccag
     6661 ttcgatgcgg ctgtcgaggt ctctgtccag cgagccatcg gcacgacgac cgcgtggacg
     6721 tgttccggca atcgggtaga tctcaatctg gcggctggtg gcgtcatact tgagcgaact
     6781 ttccggcgac gcgccaaaca gggtgaaatc attatcctgc ataaaaaaca tgtacgggct
     6841 gggattactc tttttcagca cgtaataggc cgccagcggt gacgggcagg gcagagagaa
     6901 acggcgagat ggcaccacct ggaaaatttc tccagcgcga atcgcttttt gcaacaaacg
     6961 cactacgcca ccgaactctt catcgctctg attacattca caacgcatat gcggcacgga
     7021 aaccaccggc agcggcggcg cggcttcggt cagttgctga cgtagttcgt tcaggcgagc
     7081 agtgagacgt tgtttttctt cttcattcgg agcaaacagg ctggcctgaa tacgggtgct
     7141 ttttttctga tggtcaatca ccatcagagt ttcagcgaga taaaaacaga aatcagggca
     7201 gttattttcc gctgacagtt gcggtaaatc ttcaaatccc gccacaaggt cataagagaa
     7261 caggccgccg aagaacattg cttctcgttc ttccttcggt acattcaaca gattctgcaa
     7321 taaacggaaa gcgtcaaaaa ccgaaaggga gcataagcga gcgtcttcat ccagcagtgg
     7381 actgacaggg gggaagcgca gcacacggca gtttggtaat tgttcatttt ccacacccgc
     7441 aggcagggcg ttatccagta gtgtcagcag ggcttcgccg ttgccggaaa gtgcctggat
     7501 tgtgacagtg tcacctaaag ctgtaatgcg cagcgcactg tctaccagca gcaggctttt
     7561 taaatcatct ttgctgtcga tatctgcgga ttccagcagc agcgttgccg gacgatcccc
     7621 acacaactgg tgaaaaagcg cggtgggatt gtcgcgataa gcgccttcgc aggttagcag
     7681 ttcgagagtc ggtttttgtg tttgcattgt tattctctaa ttttgttcaa aaaaagcccg
     7741 ctcattaggc gggctgggta tctgattgct ttacgcatgg tgaatacact gcccgtttca
     7801 ggaagtgcgc caccaacctt tcagtacgaa aattgctttc attgtcgata ccctttttac
     7861 gtgaacttgc gtactagtta actagttcga tgattaattg tcaacagctc atttcagaat
     7921 atttgccaga accgttatga tgtcggcgca aaaaacatta tccagaacgg gagtgcgcct
     7981 tgagcgacac gaattatgca gtgatttacg acctgcacag ccataccaca gcttccgatg
     8041 gctgcctgac gccagaagca ttggtgcacc gtgcagtcga gatgcgcgtc ggcaccctgg
     8101 cgatcaccga ccatgacacc acagctgcaa tcgcgccagc aagagaggaa atttcacgtt
     8161 cgggactggc gttgaatctt attcccggcg tggaaatttc cacggtctgg gaaaatcatg
     8221 aaattcatat tgtcgggctg aatattgata ttactcatcc gctaatgtgt gagttcctcg
     8281 cgcagcagac agaacggcgc aatcagcggg cgcagctgat tgccgaaaga cttgaaaaag
     8341 cgcaaatacc tggggcgctg gaaggcgcac aacgactggc gcagggcggc gcagtgacgc
     8401 gcggtcattt tgcgcgtttc cttgttgagt gcggcaaagc cagttcaatg gcggatgtct
     8461 ttaaaaagta tctggcgcgc gggaaaaccg gatacgttcc gccacagtgg tgtacaatag
     8521 aacaagctat tgatgtcatt catcattctg gcggtaaggc ggtgctagct catcctgggc
     8581 ggtacaatct tagtgctaaa tggctgaaaa gattggttgc gcattttgcc gaacaccacg
     8641 gtgacgcgat ggaagtcgcg cagtgccagc aatcgcccaa tgaacgtacc cagctggcgg
     8701 cccttgcgcg tcagcatcat ttatgggcat cacaaggatc tgattttcat cagccatgcc
     8761 cgtggatcga actgggtcgt aaactctggc tgcccgcagg cgttgaaggc gtctggcagc
     8821 tatgggaaca gccgcagaac accacagaga gggaattatg agccagtttt tttatattca
     8881 tcctgataac ccacagcaac gtctgatcaa ccaggcggtg gagatcgtgc gtaaaggcgg
     8941 ggtgattgtt tatccaactg attccggcta tgcgctcggc tgtaaaattg aagataaaaa
     9001 cgcgatggag cgtatttgtc gtattcgcca gctgccggac ggtcacaact ttaccctgat
     9061 gtgtcgcgat ctttctgaac tctcgaccta ttcatttgtt gataacgttg cgtttcgttt
     9121 aatgaaaaac aacacgccgg gcaactatac cttcatcttg aaggggacga aggaagtgcc
     9181 acgccgtctg ttgcaggaaa aacgcaaaac catcggtatg cgtgtgcctt ctaacccgat
     9241 tgcgcaggcg ttgcttgaag cactgggcga accgatgctt tccacttcgc taatgctgcc
     9301 aggcagcgaa tttaccgaat ccgatcccga agaaattaaa gaccgtctgg aaaaacaggt
     9361 ggatttgatt atccacggcg gctatctcgg acagaaaccg acaacggtta ttgatcttac
     9421 cgatgatacg ccggtcgtgg tgcgtgaagg cgtaggtgat gtgaagcctt tcttataagt
     9481 gcgtaactct gtatactacg cggccttaaa atggcgctat acggcgccat tgattctcct
     9541 gttcgacgcc tgtgaaggcg acaccaaaag gaagctctat gagcgaaaaa ttacagaaag
     9601 tgctggcccg cgctggccac ggctcccgcc gtgaaatcga atctattatt gaagccggtc
     9661 gtgtgagtgt tgatggcaaa attgctaaac tcggcgatcg cgttgaagtt acccctgggc
     9721 tgaaaattcg tatcgatggt catctgattt cggtacgtga gtccgctgaa caaatttgtc
     9781 gcgttctggc ctattacaaa ccggaaggtg agttgtgtac ccgtaacgat ccggaagggc
     9841 gtccgacggt gtttgaccgt ctgccaaagc tgcgtggcgc acgctggatt gccgtaggtc
     9901 gtctggacgt taacacctgt ggtctgctgc tgttcaccac cgatggtgaa ctggcaaacc
     9961 gtttaatgca tccaagccgt gaagttgaac gtgaatacgc cgtgcgtgta tttggtcagg
    10021 ttgacgacgc gaaactgcgt gatttgagtc gtggcgtgca actggaagat ggtccggcag
    10081 cttttaaaac catcaagttc agcggcggcg aagggatcaa ccagtggtac aacgtgactc
    10141 tgaccgaagg ccgtaaccgt gaagttcgtc gtctgtggga agcggttggt gtgcaggtga
    10201 gccgcctgat ccgtgttcgt tacggtgata tcccactgcc aaaaggtctg ccacgcggtg
    10261 gctggacgga gctggacctc gcccagacta actatctgcg cgaactggtg gagcttccgc
    10321 cagaaaccag ctctaaagtc gctgtagaaa aagaccgtcg tcgcatgaag gctaatcaga
    10381 ttcgtcgtgc ggtgaaacgt cacagtcagg tgagcggcgg tcgtcgttct ggcgggcgta
    10441 ataacggtta atcaaagtat gccggacgtc atatccggca tttttacaga ttaataatca
    10501 atccctattt gcgccttaac accggcatca aacgcatgtt tgatggggcg cagctcactt
    10561 accgtatccg ccagttcaag aatatcccga tgacagcctc gacccgtgat aatcaccgtc
    10621 tgttgatgtg gacgttcatt taacgcctgc accacttctt ccagtggcaa atagtcatac
    10681 gccaccatat acgtcagttc atcaagcaaa accatatcca gtgaggaatc agcaagcatc
    10741 cgctttgcat gttgccagac ttcgcggcag gcggcggtat cagactcgcg gttttgtgta
    10801 tcccaggtaa agcccgttgc catcacctga aactcaacgc catgtggctc cagcagattg
    10861 cgttcgccat taggccaggt gcctttaata aactgcacga cgcctacttt ttttccgtga
    10921 ccaactgcgc gtgttgccgt accaaatgcc gcggtggttt tgccttttcc attgccggta
    10981 aagacgatga taataccgcg ttcatcctgg gcctgggcca cacgagcatc tactttttct
    11041 ttcactcgct gctgacgctg ttggtagcgt tcatcactca ttgggaaatt cctggtttac
    11101 ggcccggttg ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc
    11161 agaggtagag cggcatgata tcagcgggtg ttttaagttt ctgtggatct tcggtcggga
    11221 aggcgctggc acgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg acgcgcaggc
    11281 gctgctgata ttcatcggcc agtacctgca tcatcccttc ggtggcaaat ttcgacgctg
    11341 catatgcgcc ccagttggct cgtccctgac gtccaacgct tgatgaagta aagaccagtg
    11401 aaccggcgtc cgatttgagt aataaaggaa gcagtgcctg ggtgagcata aaggtggcat
    11461 taacgttgac ctgtatgacg tcctgccaga cctgtggatt ttgttcgctc attgggcaaa
    11521 catcgccgag caatccggca ttatgcaaaa caccatccag acgcggataa ttaacggtaa
    11581 tgcgctgtgc cagttgttgg caatcttcgg acgtgcaggt cagcaaatcg agaataaacc
    11641 actgtggctg acgtccagtt tcttcgttta tgtggctggc tacctgacgt aatttttctt
    11701 cattacggcc caacagaatc actgtcgcac catagcgtgc atacgtcatc gcggcttcac
    11761 ggccaatccc atcgctggct cccgtcacca ggataatgcg atcattgagt aaatcttgtt
    11821 ttggctggta atgcatggct actcctcaac gacgttgtct gtcgtgctgc cagtgtactt
    11881 tatgactttg gggctttatg cctgaaacag aagggcattt caatcagcct ggggcgataa
    11941 caccgcaaaa tggatacttt gtcatacttt cgctgcaata acatctctgc gagacggctt
    12001 aacatgcctg ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga
    12061 attgttgtct gaatatggtt tgtttttggc gaaaatcgtt accgttgtgc tagcgattgc
    12121 ggcgattgcc gccattattg tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt
    12181 acgggtcaac aatctcagcg aacagtataa ggagatgaaa gaagaactgg ccgcggcgct
    12241 gatggactca catcagcaaa aacagtggca caaagcgcag aagaaaaagc acaagcaaga
    12301 agcgaaagca gcaaaagcga aagccaaact gggcgaggtg gcaactgaca gtaaacctcg
    12361 cgtctgggtg ctggatttta aaggcagcat ggacgcccat gaagtgaact cgctacgtga
    12421 agagataacg gcggtactcg cagcattcaa accgcaggat caggttgtgc tccgtctgga
    12481 aagccctggt ggcatggtgc atggttacgg gttggcggct tcgcagctgc agcgtctgcg
    12541 tgataaaaac attcctttaa ctgttacggt agacaaagtc gctgccagcg gcggttacat
    12601 gatggcctgt gtggcggaca aaattgtttc cgcaccgttt gctattgtgg gttccattgg
    12661 ggtggtggcg caaatgccca actttaaccg cttcctgaaa agcaaagata ttgatatcga
    12721 actgcacact gccgggcagt ataagcgtac tctgacgttg ctgggtgaaa ataccgaaga
    12781 agggcgggag aaattccgcg aagagttgaa cgaaacgcat cagttgttta aagattttgt
    12841 gaagcgtatg cgtccgtctc tggatattga acaggtggca acgggtgaac actggtacgg
    12901 acaacaggcg gtagagaaag gcctggttga tgaaatcaac accagtgatg aagttattct
    12961 tagcctgatg gaaggccgtg aagtggttaa tgtacgctat atgcagcgta aacgactcat
    13021 tgaccgattc accggcagcg cggcagagag cgccgatcga ttgttgctac gctggtggca
    13081 gcggggtcaa aagccattga tgtaaaagac aaacgcgagg ctaagacctc gcgttttgct
    13141 ttaatcaacc agatgatatt tttctgaaag cacatgggcc aggtgtttga acatattaaa
    13201 caccgcggtg cttttggctg ttggcaatcc ttgttcatct aaaaagtagt cgccggtaaa
    13261 taccagcacg ccattacgct gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc
    13321 atgctcacga atgattttgt tggcctcttt caacagcgtt tcgcggtcga tgggttgtgt
    13381 ctctttgttc attatttact ccttaaacaa ggacattagt ctacgccagg catggcttgc
    13441 agacaaatat accacgctgg tggcaagagc gccttactgg taactttgga ttttgcatgc
    13501 taataaagtt gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc aatctggcaa
    13561 tagatttgct tgacattcga ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc
    13621 tgttaactca gtcacctgaa ttttcgtgaa cagagtcacg acaaggggtt gatatccgca
    13681 gagaacgagt ccatatcggt aactcgttgc cagtggaagg tttatcaacg tgcgacgcat
    13741 tcctggaaga atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc
    13801 cccggcaaaa gccaaaacga tcaacaagta tctgggtagt gactacatgg tgaaatccag
    13861 cgtcggtcac atccgcgatt tgccgaccag tggctcagct gccaaaaaga gtgccgactc
    13921 tacctccacc aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg
    13981 tatgggggtt gacccgtggc acaattggga ggcgcactat gaagtgttgc ctggtaaaga
    14041 gaaggtcgtc tctgaactga aacaactggc tgaaaaagcc gaccacatct atctcgcaac
    14101 cgaccttgac cgcgaagggg aagccattgc atggcacctg cgggaagtga ttggtggtga
    14161 tgatgcgcgc tatagccgag tggtgtttaa cgaaattact aaaaacgcga tccgccaggc
    14221 atttaacaaa ccgggtgagc tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt
    14281 tatggaccgc gtggttgggt atatggtttc gccgctgcta tggaaaaaga tcgctcgtgg
    14341 tctgtctgcc ggtcgtgtgc agtcggtggc ggttcgcctg gtggtcgagc gtgagcgtga
    14401 aattaaagcg ttcgtgccgg aagagttctg ggaagtcgat gccagcacga ccacgccatc
    14461 tggtgaagcg ttggcgttgc aggtgactca tcagaacgac aaaccgttcc gtccggtcaa
    14521 caaagaacaa actcaggctg cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga
    14581 acgtgaagac aaaccgacaa ccagtaaacc tggcgctcct tttattacct ctacgctgca
    14641 acaagctgcc agcacccgtc ttggatttgg cgtgaaaaaa accatgatga tggcgcagcg
    14701 tttgtatgaa gcaggctata tcacttacat gcgtaccgac tccactaacc tgagtcagga
    14761 cgcggtaaat atggttcgcg gttatatcag cgataatttt ggtaagaaat atctgccgga
    14821 aagtccgaat cagtacgcca gcaaagaaaa ctcacaggaa gcgcatgaag cgattcgtcc
    14881 ttctgacgtc aatgtgatgg cggaatcgct gaaggatatg gaagcagatg cgcagaaact
    14941 gtaccagtta atctggcgtc agttcgtagc ctgccagatg accccagcga aatatgactc
    15001 cacgacgctg accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg
    15061 ttttgatggc tggacaaaag tgatgcctgc gttgcgtaaa ggcgatgaag atcgcatctt
    15121 accagcagtt aataaaggcg atgctctgac gctcgttgaa cttacaccag cccagcactt
    15181 taccaagccg ccagcccgtt tcagtgaagc atcgctggtt aaagagctgg aaaaacgtgg
    15241 tatcggtcgt ccgtctacct atgcgtcgat catttcgacc attcaggatc gtggctacgt
    15301 gcgagtagaa aatcgtcgtt tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct
    15361 tgaagaaaat ttccgcgagt taatgaacta cgactttacc gcgcagatgg aaaacagcct
    15421 cgaccaggtg gcaaatcacg aagcagagtg gaaagctgta ctggatcact tcttctcgga
    15481 tttcacccag cagttagata aagctgaaaa agatccggaa gagggtggta tgcgcccgaa
    15541 ccagatggtt ctgaccagca ttgactgccc gacttgtggt cgcaaaatgg ggattcgcac
    15601 agcgagcacc ggggtattcc ttggctgttc tggctatgcg ctgccgccga aagagcgttg
    15661 caaaaccacc attaacctgg tgccggaaaa cgaagtgctg aacgtgctgg aaggcgaaga
    15721 tgctgaaacc aacgcgctgc gcgcaaaacg tcgttgcccg aaatgcggca cggcgatgga
    15781 cagctatctc atcgatccga aacgtaagtt gcatgtctgt ggtaataacc caacctgcga
    15841 cggttacgag atcgaagagg gcgaattccg cattaaaggt tatgacggcc cgatcgttga
    15901 gtgtgaaaaa tgtggctctg aaatgcacct gaaaatgggg cgtttcggta aatacatggc
    15961 ctgcaccaac gaagagtgta aaaacacacg taagatttta cgtaacggcg aagtggcacc
    16021 accgaaagaa gatccggtgc cattacctga gctgccgtgc gaaaaatcag atgcttattt
    16081 cgtgctgcgt gacggtgctg ccggtgtgtt cctggctgcc aacactttcc cgaaatcgcg
    16141 tgaaacgcgt gcgccactgg tggaagagct ttatcgcttc cgcgaccgtc tgccggaaaa
    16201 actgcgttat ctggccgatg cgccacagca ggatccggaa ggtaataaga ccatggttcg
    16261 ctttagccgt aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa aggcgactgg
    16321 ctggtcagca ttttatgttg atggcaaatg ggttgaagga aaaaaataac ctttaattct
    16381 gtcaggtttt tataaacaaa gggtcgcgaa agcggccctt ttttattgca tattattttt
    16441 tcttcacacc tatacactaa ggctataaat gatatagtgg ttatagttag cacctttttt
    16501 attattaaat cgtattagtc acccgccagg tgtgacgaaa aaacgatgtt ctgatggcgt
    16561 ctaagtggat ggtttaacat gaaattacaa caacttcgct atattgttga ggtggtcaat
    16621 cataacctga atgtctcatc aacagcggaa ggactttaca catcacaacc cgggatcagt
    16681 aaacaagtca gaatgctgga agacgagcta ggcattcaaa ttttttcccg aagcggcaag
    16741 cacctgacgc aggtaacgcc agcaggacaa gaaataattc gtatcgctcg cgaagtcctg
    16801 tcgaaagtcg atgccataaa atcggttgcc ggtgagcaca cctggccgga taaaggctcg
    16861 ctgtatatcg ccaccacgca tacccaggca cgctacgcgt taccaaacgt tatcaaaggc
    16921 tttattgagc gttatccccg cgtttctttg catatgcacc agggctcgcc gacacaaatt
    16981 gctgatgccg tctctaaagg caatgctgat ttcgctatcg ccacagaagc gctgcatctg
    17041 tatgaagatt tagtgatgtt accgtgctac cactggaatc gggctattgt agtcactccg
    17101 gatcacccgc tggcaggcaa aaaagccatt accattgaag aactggcgca atatccgttg
    17161 gtgacatata ccttcggctt taccggacgt tcagaactgg atactgcctt taaccgcgca
    17221 gggttaacgc cgcgtatcgt cttcacggca acggatgctg acgtcattaa aacttacgtc
    17281 cggttagggc tgggggtagg ggtcattgcc agcatggcgg tggatccggt cgccgatccc
    17341 gaccttgtgc gtgttgatgc tcacgatatc ttcagccaca gtacaaccaa aattggtttt
    17401 cgccgtagta ctttcttgcg cagttatatg tatgatttca ttcagcgttt tgcaccgcat
    17461 ttaacgcgtg atgtcgttga tgcggctgtc gcattgcgct ctaatgaaga aattgaggtc
    17521 atgtttaaag atataaaact gccgga
//
LOCUS       DAFNBJ010000102        17075 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000102 DAFNBJ010000000
VERSION     DAFNBJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.102"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(451..1542)
                     /gene="ychF"
                     /locus_tag="L2X49_003226"
     CDS             complement(451..1542)
                     /gene="ychF"
                     /locus_tag="L2X49_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBN4798261.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1659..2243)
                     /gene="pth"
                     /locus_tag="L2X49_003227"
     CDS             complement(1659..2243)
                     /gene="pth"
                     /locus_tag="L2X49_003227"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBN4798262.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2521..2799
                     /gene="ychH"
                     /locus_tag="L2X49_003228"
     CDS             2521..2799
                     /gene="ychH"
                     /locus_tag="L2X49_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBN4798263.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2854..4533)
                     /gene="dauA"
                     /locus_tag="L2X49_003229"
     CDS             complement(2854..4533)
                     /gene="dauA"
                     /locus_tag="L2X49_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBN4798264.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4658..5605)
                     /gene="prs"
                     /locus_tag="L2X49_003230"
     CDS             complement(4658..5605)
                     /gene="prs"
                     /locus_tag="L2X49_003230"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBN4798265.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5756..6607)
                     /gene="ispE"
                     /locus_tag="L2X49_003231"
     CDS             complement(5756..6607)
                     /gene="ispE"
                     /locus_tag="L2X49_003231"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBN4798266.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6607..7230)
                     /gene="lolB"
                     /locus_tag="L2X49_003232"
     CDS             complement(6607..7230)
                     /gene="lolB"
                     /locus_tag="L2X49_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBN4798267.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLNEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7444..8700
                     /gene="hemA"
                     /locus_tag="L2X49_003233"
     CDS             7444..8700
                     /gene="hemA"
                     /locus_tag="L2X49_003233"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBN4798268.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRGQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8742..9824
                     /gene="prfA"
                     /locus_tag="L2X49_003234"
     CDS             8742..9824
                     /gene="prfA"
                     /locus_tag="L2X49_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBN4798269.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9824..10657
                     /gene="prmC"
                     /locus_tag="L2X49_003235"
     CDS             9824..10657
                     /gene="prmC"
                     /locus_tag="L2X49_003235"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBN4798270.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10654..11046
                     /locus_tag="L2X49_003236"
     CDS             10654..11046
                     /locus_tag="L2X49_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBN4798271.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTMLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11050..11859
                     /locus_tag="L2X49_003237"
     CDS             11050..11859
                     /locus_tag="L2X49_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBN4798272.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11895..12749
                     /gene="kdsA"
                     /locus_tag="L2X49_003238"
     CDS             11895..12749
                     /gene="kdsA"
                     /locus_tag="L2X49_003238"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBN4798273.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12897..13004)
                     /locus_tag="L2X49_003239"
     CDS             complement(12897..13004)
                     /locus_tag="L2X49_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBN4798274.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWCNRK"
     gene            complement(13433..13540)
                     /locus_tag="L2X49_003240"
     CDS             complement(13433..13540)
                     /locus_tag="L2X49_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrB"
                     /protein_id="HBN4798275.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13945..15045)
                     /gene="chaA"
                     /locus_tag="L2X49_003241"
     CDS             complement(13945..15045)
                     /gene="chaA"
                     /locus_tag="L2X49_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBN4798276.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            15315..15545
                     /gene="chaB"
                     /locus_tag="L2X49_003242"
     CDS             15315..15545
                     /gene="chaB"
                     /locus_tag="L2X49_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBN4798277.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15703..16398
                     /gene="chaC"
                     /locus_tag="L2X49_003243"
     CDS             15703..16398
                     /gene="chaC"
                     /locus_tag="L2X49_003243"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBN4798278.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(16442..16795)
                     /gene="ychN"
                     /locus_tag="L2X49_003244"
     CDS             complement(16442..16795)
                     /gene="ychN"
                     /locus_tag="L2X49_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBN4798279.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
BASE COUNT         4322 a         4262 c         4364 g         4127 t
ORIGIN      
        1 ataagaaaaa gcaaaaaaca tcgatattca tttttggtca atgggttttc tggcctatcg
       61 ttgttattgt gctactggtt atcattttca tcttgccagg atcgcacgta atcacgttat
      121 tcggggactt tatctgcggg atattaccct tcaggtaagt ccttttctcg ccagcatatt
      181 ggagcgtgat caatattgat cagctgtgaa gggggtgatg tgtagtggac caaaacttac
      241 gttatatcac tactaatgag atatccgtca tctgacactg atgtctttaa ttattctcgt
      301 ggttcactgt aggcaataag cacaaaagtg taggatgtta caagaatgat taggactcgg
      361 tgaaatgaaa aatccacgca attgcgtgga ttatatatac ttttgcgctc ttcatgagat
      421 ttagcgaaac ctcatgagac aacaaattaa ttagacgttg aaaaggaagt tcatcacatc
      481 gccatcttta acgatgtaat ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc
      541 ttgttcacct ttgtaagtga tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc
      601 tttttcaaaa tcagtatgga ttttgcccgc tgcctgcggc gcggttgctc caaccggaat
      661 ggtccatgca cgcacttctt tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt
      721 ataaccggca cggatcacac ggttcaggcc cggctcttcc agcccaagct cctgcataaa
      781 ctcgtcacgt tcttcgtcgt ccagttcggc aatgtctgct tcaacagcag cacaaaccgg
      841 aaccacaaca gaaccttctt tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt
      901 ttcaaaaccg tcttcgttga cgttggcgat gtacattgtt ggtttcagcg tcaggaagct
      961 caggtaacga atagccgctt tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc
     1021 attttccaac tggggcaggc atttttccag gaccgccagc tcagctttcg cgtctttatc
     1081 gccacctttg gctttcttct gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc
     1141 cagcgccagt tcggtgttga taacttcaat atcgtcagcc gggttaactt tgccggaaac
     1201 gtgaatgatg ttgtcatttt caaagcagcg aacaacgtga ccgatcgctt cggtttcacg
     1261 gatgttggtc aggaactggt tacccagacc ttcgcctttc gatgcgcctt ttaccagacc
     1321 ggcgatatcg acaaattcca tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc
     1381 cagttgatcc aggcgagggt caggcattgg tacgacgcct gtgttcggct caatggtgca
     1441 gaatggaaag ttggccgctt caataccggc tttggtcagc gcgttgaaca gggtagattt
     1501 cccgacgttg ggcaaaccga cgataccgca tttgaatccc atgatttaaa tcaccttaat
     1561 atcttaataa tcaacctgtt attgctaaca gattgcagaa atggaaataa ctttgcctat
     1621 tatacacggc actcggcaaa aatgccgcag acaacgactt attgcgcttt aaaggcgtgc
     1681 aatcggttcg ttgctttggt caagccatct gtaaaccaca tttcagtaca acgcgccgct
     1741 tcgtcgatgg cttcatcaat taacttctgt tcactaacag gcggtttgcc taacacaaaa
     1801 ccgacaactt tatttttatc gcccggatga ccgattccga tgcgtaaacg gtgaaagtta
     1861 gggttattac ccaatttact gatgatgtct ttcagtccat tgtgaccacc atggccaccg
     1921 cccaatttaa atttggcgac gccaggaggc agatccagtt cgtcgtgggc caccagaatt
     1981 tcgtccggat taatgcggaa aaaactggcc atcgccgcaa cggctttgcc gctgagattc
     2041 ataaatgtag tcgggactaa caggcggaca tcttcgcctc caagagtgac tcgcgaagta
     2101 taaccaaaga atttagcctc ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca
     2161 acgaaccagg caccagcatt atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg
     2221 ccgacaatca atttaatcgt cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta
     2281 gtttactggt tgcggccccg cttgacaaaa aactgcgtat caaatgcaga taacgtaata
     2341 attgcatgag tggactatta gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt
     2401 tgaaataagg gttgtaattg tgatcacgcc cgcacataac ccacagggtg ttgtctatac
     2461 tttacacata aggaagaggg gtattccctg ttacaaccca gaaagttccg gaggtgacat
     2521 atgaaacgca aaaacgcttc gttactcggt aacgtgctca tggggttggg tctggtggta
     2581 atggtggtcg gcgtggggta ttcaatcctc aaccagttac cacagtttaa tatgccccag
     2641 tatttcgcac atggtgcagt gctaagtatt ttcgtcggtg ccattctctg gctggcgggt
     2701 gcccgtgttg gcgggcatga acaggtgtgc gaccgttact ggtgggttcg ccactatgac
     2761 aaacgttgcc gccgtagcga taatcgccgt catagctaac aaataatgca gtttgctgac
     2821 cagtcagttt gcgctgactg gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg
     2881 attcgggaag aacgccaggc gtcccgggat cggttgaatg ccagcgcgag ccatagtgcg
     2941 cagtggctgg aattccacgt tgcacacgcg cagttcacat ccctcgggca gacgcttcac
     3001 aaaacgctgg aacgcatcaa gaccaccagc atcaagtacc ggaacggcat cccacttcag
     3061 aatcacaatc cgtttgcctt caagacgtga ctccaggtcc gtgaacaagc cttcagcagc
     3121 agcaaaaaac agcgggccaa taacacgcag aaccaggaca tcgtctggaa catctacgac
     3181 taccggtgcc aggcgagtca tacgtgcgat acgacgcata aacagcagcg atgccagcac
     3241 gatccccacg ctgatggcaa taaccatatc aaacagcacg gtcaacgaca tgcacagcag
     3301 catgacaatg atgtcatctt tcggcgcatg acgcagcaag tcgaccactt tgtgcgcttc
     3361 actcatgttc cacgccacca tcaacagcag ggctgccatg gcggaaagcg gcagccagga
     3421 gagcagcggt gccagtacca gcagggcaag aataaccaga atagagtgga tcaccgccga
     3481 gataggggaa gttgccccgg cacggacgtt agcggcagaa cgcgcgatgg cagctgtagc
     3541 ggtaatacca ccaaagaacg gagcgataat attccccagt ccctgtccaa ccagttcgct
     3601 gtttgccttg tgttttgtcc cggtcatacc atccagcacc acggcgcaga gcagagattc
     3661 gattgcgccg agcattgcca ttgagaatgc cgcaggcagc agtgtgcgaa tagaatccca
     3721 ggtaagcgtg aattctgaat taggcagatc ccacggcagc accagttgcg gcagcagttg
     3781 cggaataccg ttaccctgag aaccatcggc caggacgtag tggaattgcg aaccgatggt
     3841 agcaacatgt ccgccgagca ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc
     3901 cggaaggtga ccgggtaaac gaatgcccag acgcggccag aaaaccagaa tgccaagcgt
     3961 cacaatgcca atggcagcat cacccacatt aatggtcggc agcgccataa ataatgcgcc
     4021 gactttttgt agataatgtt ccgggacatg ggccatttgc agaccgagaa aatctttaat
     4081 ctgcatggta ccgatggtga tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg
     4141 aatatactca atcaggcgac caaagcgtgc cagacccatc agaatcaaaa agatccccga
     4201 cagcaaggtc gcaaccagca gtcctgccag tccaaactgt tgcgacacgg gatagagaat
     4261 taccacaaat gccgcagtcg gaccggaaac gctaaagcgt gatccacccg tcagagcaat
     4321 gacaatcccc gcaacagctg cagtatataa accgtactgg ggtgccacac cactaccaat
     4381 agccaacgcc atcgccagcg ggatagcaat aatcccgacg gttatcccgg caatcaggtc
     4441 acgggtaaac cgtgcggcag tatatttttc tttccagcaa gcgtcgatca gagcgcggaa
     4501 aggcatcaca tgtgaggaaa atattttgtt cacaataatg tttcatccgt gagcgcatca
     4561 tctgtcaact aaatggcagg tgaaggaggc ataggtcata caaatggata ttacagacaa
     4621 aaaaacccgc cgcagcgggt ctttgagccg ggttcgatta gtgttcgaac atggcagaga
     4681 tcgattcttc gttgctgata cgacgaatcg cttcggccag catacctgac agggtcagag
     4741 tacgcacgtt cggcagtgat ttgatttcat cgctcagcgg aatggtatcg cagacaacga
     4801 cttcatcaat tacagagtta cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag
     4861 tcgcgtacgc aaatacacgt ttagcaccac gttctttcag agcttcagca gctttacaca
     4921 gcgtaccgcc agtgtcgatc atatcatcga ccagtacgca gtcacgacct gcaacgtcac
     4981 cgatgatatg catcacctgg gaaacgttcg cacgcggacg acgtttgtcg atgattgcca
     5041 tatcggtatc gttcagcagc ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg
     5101 gagaaaccac aattgggtta tccagattca gctgcagcat gtcttccagc aggatcgggc
     5161 taccaaatac gttatcaacc ggaacgtcga agaaaccctg aatctgttca gcgtgcagat
     5221 ccactgtcag cacacggtca acaccgacgc tggagaggaa gtctgcaacc actttcgcag
     5281 tgattggtac acgagcggaa cggacgcgac ggtcctggcg cgcatagcca aagtagggga
     5341 taacagcggt gatacgacct gcggaagcac gacgcagggc atcaaccata acgactaatt
     5401 ccatcaggtt gtcgttagta ggggcacaag tggactggat gatgaaaata tcaccaccgc
     5461 gtacattttc attaatttgt acgctgactt cgccgtcgct aaagcgacct acagcggcgt
     5521 cgccgagtga agtgtacagg cggttggcaa tacgttgtgc tagttccggg gtggcgttac
     5581 cagcaaaaag cttcatatca ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa
     5641 gaatctgccg aaaactgtgc gggccaggca tgatcctttc caggcggtgt atttaagagc
     5701 gcgatgcaac gtctggaaca aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa
     5761 gcatggctct gtgcaatggg gaaagattaa cgcctttcgc cacaaagcca ttgagccatt
     5821 ccggggcttg ctctagcacc tggcgggctt cagactctgt atcaaattca gcaaagacac
     5881 aggcccctgt cccagtcagg cgcgacgggg cgtattctaa cagccaggaa agcaccgcat
     5941 caacctcgcg aaaacgtttt cttgcgataa cctcgcaatc attgctgaat tcacatttta
     6001 gcaacgtttc tattgacctt tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca
     6061 ccggagtcgg aatacttaca ccagggtgcg ccaccagata ccacttctct ggcggatcca
     6121 ccggcgttag tatttcacca acgccttcgg caaacgcggc atgcccccga acaaagacag
     6181 gaacatctgc gcccagcgtc agccccattt ccgccagctc atccatgctt agcccgcatt
     6241 gccagagatg atttaatgcc accaggaccg tcgcggcatt ggatgaacca ccgccgagac
     6301 cgccgcccat tggcaaacgc ttgtcaatgc tgatatccgc accgcttccc gtcggaagac
     6361 gcccgctgtc tgccgcagtt ttcatcaaca accgcgctgc gcgaacgatc aggttatctt
     6421 catgttccac gccttcaacg ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa
     6481 tgctaatggt gtcgccgtaa tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat
     6541 ccgcacgctg accggtaatg tataaaaaca gattaagttt tgccggagag ggccactgtg
     6601 tccgcattat ttcactatcc agttatccat ttttaacttg atgcgttgac caccgtcggt
     6661 gagttccata ttggctggca tcgcaggttg cgttttggtg tcataaccac cgtaaacaac
     6721 tttccagttt ttgccattct ggctgtaggt aatttcgttc aggcggtact ggtcgtccag
     6781 tttgtagtcg gttgcatcac ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg
     6841 aatcggcatt ccggtcaatt tgccaatcat ctcttcggcg tcatcggcgg tataacgctg
     6901 acctttattg tcgactaact gcacgttacc cggttgagca ttcagctcaa gttccgtact
     6961 gcccaatggg ttagtcagca gcagacggta gcgatcctga ccagtttgct gccagaagaa
     7021 acgggcgtac actttttgtt ggtcagaaat ataagcgaac gcgccgcgag tttgatactg
     7081 attaagattg cgcacgtctt gctgatgctg acgccattgt ggcgaatccg ggcttttacc
     7141 aggacctttg ggcgtggtaa cggaacaggc agtgagcaca agagcagcca gcggtagcag
     7201 gcggataaga cgaaaatcgg gcaggggcat agtgatgaca agtccttgag atacgttgca
     7261 gttataaccc ttaatgctag cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat
     7321 tgccagaatc taacggcttt cggcaattac tccaaaaggg ggcgctctct tttattgatc
     7381 ttacgcatcc tgtatgatgc aagcagacta accctatcaa cgttggtatt atttcccgca
     7441 gacatgaccc ttttagcact cggtatcaac cataaaacgg cacctgtatc gctgcgagaa
     7501 cgtgtatcgt tttcgccgga taagctcgat caggcgcttg acagcctgct tgcgcagccg
     7561 atggtgcagg gcggcgtggt gctgtcgacg tgcaatcgca cggaacttta tcttagcgtt
     7621 gaagagcagg ataacctgca agaggcgtta atccgctggc tttgcgatta tcacaatctt
     7681 aatgaagaag atctgcgtaa aagcctctac tggcatcagg ataacgacgc ggttagccat
     7741 ttaatgcgtg ttgccagcgg cctggattca ctggttctgg gggagccgca gatccttggt
     7801 caggttaaaa aagcgtttgc cgattcgcaa aaaggccata tgaaggccag cgaactggaa
     7861 cgcatgttcc agaaatcttt ctctgtagcg aaacgcgttc gcactgaaac agatatcggt
     7921 gccagcgctg tgtctgtcgc ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg
     7981 ctctctacgg tcacagtgtt gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt
     8041 catctgcgcg aacataaagt acagaagatg attatcgcca accgcactcg cgaacgtgcc
     8101 caaattctgg cagatgaagt cggcgcggaa gtgattgccc tgagcgatat cgacgaacgt
     8161 ctgcgcgaag ccgatatcat catcagttcc accgccagcc cgttaccgat tatcgggaaa
     8221 ggcatggtgg agcgcgcatt aaaaagccgt cgcaaccaac caatgctgtt ggtggatatt
     8281 gccgttccgc gcgatgttga gccggaagtt ggtaaactgg cgaatgctta tctttatagc
     8341 gttgacgatc tgcaaagcat catttcgcac aacctggcgc agcgtaaagc cgcagcggtt
     8401 gaggcggaaa ctattgtcgc tcaggaaacc agcgaattta tggcgtggct gcgagcacaa
     8461 agcgccagcg aaaccattcg cgagtatcgc ggccaggcag agcaagttcg cgatgagtta
     8521 accgccaaag cgttagcggc ccttgagcag ggcggcgacg cgcaagccat tatgcaggat
     8581 ctggcatgga aactgactaa ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc
     8641 gcccgtgacg gggataacga acgcctgaat attctgcgcg acagcctcgg gctggagtag
     8701 cagtacatca ttttcttttt ttacagggtg catttacgcc tatgaagcct tctatcgttg
     8761 ccaaactgga agccctgcat gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc
     8821 aaactatcgc cgaccaggaa cgttttcgcg cattatcacg cgaatatgcg cagttaagtg
     8881 atgtttcgcg ctgttttacc gactggcaac aggttcagga agatatcgaa accgcacaga
     8941 tgatgctcga cgatcctgaa atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag
     9001 aaaaaagcga gcaactggaa cagcaattac aggttctgtt actaccaaaa gatcctgatg
     9061 acgaacgtaa cgccttcctc gaagtccgtg ccggaaccgg tggcgacgaa gcggcgctgt
     9121 tcgcaggcga tctgttccgt atgtacagcc gttatgccga agcccgccgc tggcgggtag
     9181 aaatcatgag cgccagcgag ggtgaacatg gtggttataa agagatcatc gccaaaatta
     9241 gcggtgatgg tgtgtatggt cgtctgaaat ttgaatccgg cggtcatcgc gtgcaacgtg
     9301 ttcctgctac ggaatcgcag ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc
     9361 cagaactgcc tgacgcagaa ctgccggaca tcaacccagc agatttacgc attgatactt
     9421 tccgctcgtc aggggcgggt ggtcagcacg ttaacaccac cgattcggca attcgtatta
     9481 ctcacttgcc gaccgggatt gttgttgaat gtcaggacga acgttcacaa cataaaaaca
     9541 aagctaaagc actttctgtt ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc
     9601 aacaggccga agcgtctacc cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta
     9661 accgtactta caacttcccg caggggcgcg ttaccgatca ccgcatcaac ctgacgctct
     9721 accgcctgga tgaagtgatg gaaggtaagc tggatatgct gattgaaccg attatccagg
     9781 aacatcaggc cgaccaactg gcggcgttgt ccgagcagga ataatggaat atcaacactg
     9841 gttacgtgaa gcaataagcc aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat
     9901 cctgctggaa catgttaccg gcaaagggcg tacttttatc ctcgcctttg gtgaaacgca
     9961 gctgactgac gaacaatgtc agcaacttga tgcgctactg acacgtcgtc gcgatggtga
    10021 acccattgct catttaaccg gggtgcgaga attctggtcg ttgccgttat ttgtttcgcc
    10081 agcgacctta attccgcgcc cggatacgga gtgtctggtg gagcaggcac tggcgcggtt
    10141 gcctgaacaa ccttgccgta ttctcgatct cgggacgggt accggggcga ttgcgcttgc
    10201 gctggctcgc gagcgcccgg actgcgaaat taccgctgta gatcgtatgc ccgatgctgt
    10261 cgccctggca caacgtaatg cccagcatct ggcgatcaaa aatatccaca ttctgcaaag
    10321 cgactggttt agcgcgctag ccgggcagca gtttgcgatg attgtcagca atccgccgta
    10381 tattgacgag caggaccctc atcttcaaca aggcgatgtc cgctttgagc cgctcactgc
    10441 gctggttgcg gcagacagtg gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa
    10501 cgcgctggta tccggcggct ttctgcttct ggaacatggc tggcagcagg gcgaagcggt
    10561 gcggcaggca tttatcctcg cggggtatca tgacgtcgaa acctgccgtg actatggtga
    10621 taacgagcgc gtaacgctcg gccgctatta tcaatgacaa gtttttctac actgcttagt
    10681 gttcatctta ttagtatcgc gctttctgtt gggctattaa ccttacgttt ctggctacgt
    10741 tatcagaagc atccacaggc atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat
    10801 acgatgttat tgttaagcgg cattgcgttg atggctaaag cgcacatcct gccattttct
    10861 gggcaggcac agtggctgac tgaaaagctg tttggagtta tcatttatat cgttttgggt
    10921 tttattgcac tcgattatcg tcgtatgcac agtcagcagg cgcgcattat tgccttcccg
    10981 ctggcgttgg tggtgctgta catcatcatt aaactcgcca ccacaaaagt accgttactg
    11041 gggtaagtca tgagatcgtt agctgatttc gaatttaata aagcgccatt gtgcgaaggc
    11101 atgatcctgg cttgcgaagc aatccgccgc gattttccct cgcaagatgt ttacgacgaa
    11161 ctggagcgtc tcgttagtct ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag
    11221 cagctggaaa aactgatcgc gctgttttac ggcgactggg gatttaaagc ctcacgcggc
    11281 gtttatcgtc tttccgatgc attatggctg gaccaggtgt taaagaatcg acagggcagt
    11341 gcggtatcat taggtgcggt tttattatgg gtcgcgaatc gtctcgattt gccgctgctg
    11401 ccggtgattt tccctacgca gctgatattg cgcattgaat gtccggatgg cgaaatttgg
    11461 ctgattaatc cttttaacgg tgaatcgtta agcgaacata tgctggacgt atggttaaag
    11521 ggaaatatca gcccgtcggc ggaactgttt tatgaagacc ttgatgaagc tgataacatt
    11581 gaggtaatcc gcaaattgct ggatacactc aaagcctcgt tgatggaaga aaatcagatg
    11641 gagctggcgt tacgcaccag cgaagcttta ttacaattca accctgaaga tccctatgaa
    11701 attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat
    11761 ttaagttatt tcgttgaaca gtgtccggaa gacccgatca gcgaaatgat ccgtgcgcaa
    11821 ataaataaca tcgcgcataa acatattgtg ctgcattaat taatcgacat tttactcaag
    11881 attaaggcga tcctatgaaa caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg
    11941 acctgccgtt cgtactgttt ggtggtatga acgtgctgga atctcgcgat ctggcgatgc
    12001 gcatttgcga gcactacgta accgtgaccc agaaactggg tatcccttac gtgttcaaag
    12061 cctcttttga caaagccaac cgctcctcca tccactctta tcgtggaccg ggcctggaag
    12121 aagggatgaa aatcttccag gaattgaagc agacttttgg cgtgaaaatt atcaccgacg
    12181 ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg
    12241 cgtttcttgc tcgccagact gacctggttg aagccatggc gaaaaccggt gcggtaatta
    12301 acgtcaagaa accacagttt gtcagcccgg gacagatggg taatatcgtt gataaattca
    12361 aagaaggcgg caacgaaaaa gtgattcttt gcgatcgcgg tgctaacttc ggctatgaca
    12421 acctggttgt cgatatgctg ggcttcagca ttatgaagaa agtgtctggt aactcgccgg
    12481 tgattttcga cgtgacccac gcactgcaat gccgcgatcc ttttggcgca gcttccggcg
    12541 gtcgtcgtgc tcaggtggct gagctggcac gagccggtat ggcggtaggt ctggcggggc
    12601 tgtttattga agcgcatccg gatccggaac atgcgaaatg tgatggtcca tccgcgctgc
    12661 cgctggctaa actggaaccg ttcctcaagc agatgaaagc gattgatgat ctggtgaaag
    12721 gtttcgaaga actggatacc agcaagtaat ctttttgttt gaaaaataaa gtcttagcgt
    12781 tctgcgttaa gacttttttt atgggtgccg gatacaaaaa aggccgcagg ctgttacccc
    12841 tgcggccggt ttcgggcgca tattgccatc acggcagcct gacgcccgtt ttcgccttac
    12901 ttccggttac accaccagcc gacaatcgct gcggtaataa ttcccgccag gatcggtgct
    12961 gccaggtcgt gccagaaaat catggcaaac tgcgcgagcg tcatatagcc gccttgttgt
    13021 aatgacaaca ttttgcggct attcttgaat tgttgatggt tcaagattag cccccgttct
    13081 gttgtcaggt tttacctctc aacgtgcggg ggttttctct ttccagcaac caatgccacc
    13141 agggataaag cccccgcaac attgcgcctc accggataac gccggcttgg tgtggatact
    13201 acgactcaat tcatcttcac ttcatccctg aaatgtttgc aatgaagagt gcattccggt
    13261 ttttcaacag ctgttacagt cgtttcatga gtgctctgga tgatgcttcc agctcgggtt
    13321 gccaatattt acttgtggaa gtgataaaga caaaaatggc cgcaggctgt tacccctgcg
    13381 gccggtttcg ggcgcatatt gccatcacgg cagcctgacg cccgttttca ccttacttcc
    13441 ggttacgcca ccagccgaca atcgctgcgg taataattcc cgccaggatc ggtgccgcca
    13501 ggtcgtgcca gaaagtcatg gcaaactgcg cgagcgtcat atagccgcct tgttgtaatg
    13561 acaacatttt gcggctattc ttgaagtgtc tggtttcaag attagccccc gttttgttgt
    13621 caggttttac ctctcaacgt gcgggggttt tctctttcca gcaaccaatg ccaccaggga
    13681 taaagccccc gcaacattgc gcctcaccgg ataacgccgg cttggtgtgg atactacgac
    13741 tcaattcatc ttcacttcat ccctgaaatg tttgcaataa agagtacatt ccggcttttc
    13801 aacagctgtt gcagtggttt cgtgcaggtt gtggtacagg ctcgcaattc tgattaacga
    13861 cctgtccagg tagtatgaag aaatacaata agcaggaatt tatattatct cccccgattt
    13921 ccgtcatcgg aggagatata actgtcaggc aaatatcgtc atcaaatagg cggcaaacag
    13981 tgccagatgc gctgcgccat tgagcacgtt agtacgtccg gtggagaagg agatatggca
    14041 cagcactaaa gaggccacca tcaccaccat ttctggcgca ccaagtgcaa actgtaattc
    14101 gttacccgtc ataaaggcaa ttagcgtgac gacaggtacg gtaagcgaaa tggttgctaa
    14161 cactgaacca aagaacagat tcatcgcgcg ctggacctgg ttgttcaaca ctgcttttaa
    14221 cgcacctaaa ccttccggcg acagaatcaa cagagccacc aggaagccag taaaggcgac
    14281 aggggcattc atgctgtcga gcaatgtctc cagcgggctg gcattcattt tggtcaccgc
    14341 aataacggca atcagatgga taatcaacca gatagcatgc cacacgctgc tatgggcaga
    14401 cggtttaccg tgatgcgggt catcgtcatc actgtcatct tcgtgctcgt agacaaacaa
    14461 actttgatgc gttttggtct ggatcagcaa aaatacgcca tacattgccg cagaaattaa
    14521 tgctaccagt aacgcctgac cggttgaaaa attcgccgca ggcagagcca ttggaaatac
    14581 cagtacgatt atcgccaggg ggaacagcgc aattaaatac tgcttgatac caaacagatt
    14641 catatattgg gtggcaaact tacgaccgcc caacaataat gaaaagccaa ccagcccacc
    14701 ggtaacaatc ataatgattg aatagagcgt atcacgcatt agcgttggcg cggcgtcgcc
    14761 ggttgccatt aaagctgaaa tcaaactgac ttcaagaata accactgaaa ggctaagaat
    14821 aagcgaaccg taaggttctc ccaggcgatg ggctaatacg tccgcatgac ggacaacact
    14881 aaaggcgcta cttaaaatac caataagcgc aagaagattg atggcaatga ccactggtag
    14941 tgtctggctg cttccccaca ggaacagcac taccagcgcc agaaccggga aaataagcga
    15001 agtctccttg tggcgggttt ttaccgcctc ttgagcattt gacattatgg ttatcccttt
    15061 gcagatgaat ttatcgaaaa tgtaaaaaat aggtaggaaa aataacagaa agtgtctgga
    15121 tatcggtaac attttacgaa tttttacccc actgtcgatt ttttactcat tgggacataa
    15181 aaataagtat tacgtttaga caatgtttgt tttagcgttt cttaagaaca gtctgacatg
    15241 aaaattctta tgttttggca agagtagata ttgttgacca cacttaatgt tcaactttgt
    15301 aaaaggagtc aacgatgccg tataaaacga aaagcgatct gccggaaagc gtaaagcacg
    15361 ttctaccgtc tcatgcccag gatatctata aagaagcgtt caacagcgca tgggatcaat
    15421 ataaagataa agacgatcgg cgtgatgacg ccagtcgcga agaaaccgcg cataaagtgg
    15481 cctgggctgc tgtgaagcat gaatatgcca aaggggatga tgataaatgg cataaaaaat
    15541 cgtaaaaccg gtagcttagt taaagctatt cgtgcggtgt tgccttgcaa gtggtccgtg
    15601 gattgcatat tgtcccgtta gtggtttcaa aatgagcagt aaaaatgtcc ggaagacacc
    15661 aaaaagttgt cgcagggaag tatgcagtgg cggaggtgta aggtgataac gcgtgatttc
    15721 ttgatgaatg ccgattgtaa aacggcattt ggtgccattg aagaatcact cttatggtca
    15781 gcagagcaac gggcggcttc gctggcggcg acgctggctt gtcgacctga tgagggaccg
    15841 gtgtggatct tcggttatgg atcgttgatg tggaatccgg cactggagtt taccgagtcg
    15901 tgcaccggta cactggttgg atggcatcgc gcgttttgcc tgcgcctgac cgccgggcgc
    15961 gggactgcgc accagccggg acggatgctt gcactgaaag agggcggacg cactacaggc
    16021 gtcgcctatc gactgccaga agagacgctg gagcaggaac taaccctgtt gtggaagcgc
    16081 gagatgatta ccggctgtta tctgccaacc tggtgtcagc ttgatcttga tgatggatgc
    16141 acagtaaacg ccattgtgtt tattatggac ccgcgacatc cagaatatga atctgacact
    16201 cgcgctcagg tcatcgcgcc gttgattgcg gcggcgagcg gtccgctggg aaccaacgca
    16261 caatacctgt tttcactgga acaggagctt atcaaactgg gaatgcagga cgatgggctg
    16321 aatgatttgc tggtatcggt aaaaaaactg ctggcggaga attatccgga tggtgtgtta
    16381 cggccgggat tcgcctgagt aaacttccct catagtgggg cgtcagacgc cccctcaaac
    16441 attaaaatgt gagcacttta tcggctgaca gcgtccattg cgccagttcc acaagagtac
    16501 cgatttccac cccatcaata agaggaagtg tgctaatccc gcgcccgtcg gtacaggttt
    16561 tgcacaattt caccggtaca ttctgagcgg taaggatctc cagcatttgc tgaatgttgt
    16621 agccttcccc tggtttttgc ccgcgcaacc cggctgtgac cgcatcagac atcaggaaca
    16681 gacgcagatc cagattgctc tcctgctctc gtaacgcaat ggccagccgc aagctgttaa
    16741 acaaggattc gctcccgtaa ggtgcgccat tggcaacgat cacgattttt tgcattattt
    16801 actcctgtat tcagggaatt agacattcat cttctatctt actgcttctg cagcgtctga
    16861 ccaatcggtc acatttttaa ggattttcct gaaagcgcga gaaaatacga caaaagttgc
    16921 cagtaatcgt tattctttaa ggctatggtt tttcattatt accggaagtt accgacgttt
    16981 tgagccgttt cgttcctcgc attatcccgt tttatttact cttgcttgta gcaggcggta
    17041 cagctaacgc acaatctacc ttcgagcaaa aagcg
//
LOCUS       DAFNBJ010000103        17062 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000103 DAFNBJ010000000
VERSION     DAFNBJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.103"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..540
                     /locus_tag="L2X49_003245"
     CDS             <1..540
                     /locus_tag="L2X49_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HBN4798280.1"
                     /translation="AIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(576..1532)
                     /locus_tag="L2X49_003246"
     CDS             complement(576..1532)
                     /locus_tag="L2X49_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBN4798281.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1532..2773)
                     /gene="clsB"
                     /locus_tag="L2X49_003247"
     CDS             complement(1532..2773)
                     /gene="clsB"
                     /locus_tag="L2X49_003247"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBN4798282.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2770..3531)
                     /locus_tag="L2X49_003248"
     CDS             complement(2770..3531)
                     /locus_tag="L2X49_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBN4798283.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3664..4074
                     /locus_tag="L2X49_003249"
     CDS             3664..4074
                     /locus_tag="L2X49_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBN4798284.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRPPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4036..5142)
                     /locus_tag="L2X49_003250"
     CDS             complement(4036..5142)
                     /locus_tag="L2X49_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4798285.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5153..6286)
                     /locus_tag="L2X49_003251"
     CDS             complement(5153..6286)
                     /locus_tag="L2X49_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4798286.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6279..8015)
                     /locus_tag="L2X49_003252"
     CDS             complement(6279..8015)
                     /locus_tag="L2X49_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN4798287.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVDGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8008..9003)
                     /gene="hlyD"
                     /locus_tag="L2X49_003253"
     CDS             complement(8008..9003)
                     /gene="hlyD"
                     /locus_tag="L2X49_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBN4798288.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLILA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(9006..9677)
                     /gene="cecR"
                     /locus_tag="L2X49_003254"
     CDS             complement(9006..9677)
                     /gene="cecR"
                     /locus_tag="L2X49_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBN4798289.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9906..11270
                     /gene="rhlE"
                     /locus_tag="L2X49_003255"
     CDS             9906..11270
                     /gene="rhlE"
                     /locus_tag="L2X49_003255"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HBN4798290.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11502..11984)
                     /gene="ybiA"
                     /locus_tag="L2X49_003256"
     CDS             complement(11502..11984)
                     /gene="ybiA"
                     /locus_tag="L2X49_003256"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBN4798291.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12104..14254
                     /gene="dinG"
                     /locus_tag="L2X49_003257"
     CDS             12104..14254
                     /gene="dinG"
                     /locus_tag="L2X49_003257"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBN4798292.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14282..15244
                     /gene="ybiB"
                     /locus_tag="L2X49_003258"
     CDS             14282..15244
                     /gene="ybiB"
                     /locus_tag="L2X49_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBN4798293.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15385..16470
                     /gene="hcxB"
                     /locus_tag="L2X49_003259"
     CDS             15385..16470
                     /gene="hcxB"
                     /locus_tag="L2X49_003259"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBN4798294.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(16699..16959)
                     /locus_tag="L2X49_003260"
     CDS             complement(16699..16959)
                     /locus_tag="L2X49_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBN4798295.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
BASE COUNT         3971 a         4528 c         4621 g         3942 t
ORIGIN      
        1 gccatagggt tattattagt ttgtatgtat ggcttaagta aggatatcaa caagataaat
       61 gccgtcatcg caggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca
      121 agtttagctc ctgtacctgc cattatcgtc gtgttcagca ctgcgggcgc gatgttttta
      181 atcagtatgc tggccggttt attatttaat gttgatcctg gttctcaccg ttttatcatt
      241 atgatgacgt tgacagggtt ggccctggtg atcatcatga atgccgcatt aatgagtgaa
      301 cgacccgttt gggtaataag ttgcttaatg attgtgttat ggtcaggcat tatctcgcat
      361 ggacgaaata agctccttga attggcgggg aaatgccata gtgaagagtt gtggagtccg
      421 gttcgttgcg ctgttacagg tgcattaaca ctctattact attttatcgg cttctttggg
      481 atacttgccg cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag
      541 tcgtaaatgc agatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc
      601 cgtagcttct tcgcctggct ttccagtagc aggtaacaga tcagcgccag cagcagcggg
      661 ataaagtaat acaggacgcg ataagcgagc agggcggcga taatgctccc tttcgaggta
      721 tgctccccag ccagtagtgc gataaacacc gcttccagca caccgatccc cgccggaata
      781 tgcacgatga cgccagcaat actactaacc agtaacacgc ccagtacaaa gaaatagttc
      841 acgctttgac caagtaacag ccagataatc gcccccatta ccatccagtt aacgctggaa
      901 atcagcatct gagcgagggc gaatttccat gaaggcagca ccagcttttg ccctttgatg
      961 gtcatatggc ggtgcttcgc gaaagcgcaa aaccacaaat aaaccgcgat aatcatcagt
     1021 aagccaatgc cgagaatacg cagcgtagtt tgatcgacat accagtgatc cggcaactcc
     1081 accacgcctg cggtaaagat aatccctgcc agtaaaatat agcccagcca gttggtggta
     1141 atactgagcg agaaaatccg cgtaatagtg ctaccgggca accccagacg agagtacaaa
     1201 cgatagcgca taccaatgcc gccgacccag gtactgagcg tcaggttgaa ggcgtagcag
     1261 ataaacgaca ccagcatcac ctggcgcttc gccagtttgt gaccgcagta aaagcgggcg
     1321 agcaggtcat agcagccgta aatcagatag ctgacgacca ccagcccgac cgcactaagc
     1381 agcgcaacgc gattgtagtc gcggatgacc ttccagacct cttcccagtc cacttttttg
     1441 gcgtagacca ccagcaacac aatcaccgcg ataaaaaaca gccaggtgag gatcttcttt
     1501 gctaagcgcc agcgcgggtg tgatttactc atcagggttt tacccccgtg ttttcagttt
     1561 ctacccgatc ctgcgtttcc attgtcggtt gtgcgggcgg atcaacctgc gccagacgtg
     1621 gcgtgtgtgc cggaagccag ccaaccagcg ccgggaagtg gcgtaaaaag tggaacgcca
     1681 gcacgctttt ggtcaggttc caccaggtgc gtttgggcag catggtttca tccacctgct
     1741 gacaatctgc ggcgataatg ccgttcagat tatcgcgcag cgtctggtta aaattacgat
     1801 cgtggatgat gacatttgct tcgagattca gtgacaaact gagcggatcg agattactgg
     1861 accctactgt tgcccagtga tcgtccatca atgctacttt gccgtggagc gggcggcggc
     1921 ggtactcaaa cacctgaacg ccgcctttaa ccagatagtt atacagcaag cgcgcgccga
     1981 ctctgacaat cggcatatcc ggttcgccct gaatgatcag tttgatccgc accccgcggc
     2041 gtgccgcttt acgcaaggcg tgtaaaaatc gatagccggg gaagaagtag gcgttggcga
     2101 taatgacttc ccgccgcgcc tgagtgagca ttttcagata gtggcgttct atatcatcgc
     2161 ggtgttcttc gttatcgcgc cagaccagca atacctgcgc ttctcccggc tggcgatttt
     2221 cttccgcttt gtgatgacgt cgccaccagc gtcgtgccgc gctctgtcca ggcaggtttt
     2281 ccagctcaaa ctggagaata tcttcgacaa tcggcccttc aaggcgtacc gcgtaatcct
     2341 gtttagcctc tggaccgtag ctggacatat gctcggcgga gtaattcagc ccgccaataa
     2401 aggcgatacg cgcgtcgatc accacaattt tgcgatgcat ccggcgaaac acattggtgc
     2461 gcataccaaa aaggcgaggg cggggatcgt agtagcgaaa taccacgcca gctgccgtca
     2521 gttcattgac aaactcatcg ctgagatccg gcgaaccgta gccatccagc aagacttccg
     2581 ctttaacccc gcgttgcgct gctgccagta gtgccgcatg cagctgtttg ccgacgtcat
     2641 cctcaaacca gataaacgtt tcaagaatga tgcgttcttg tgcctcgcca atcgccttaa
     2701 acaccgcggg ataatattgc tcgccgtttt ccagcaactg gatcttattg ccttcgcgcc
     2761 agctacattt cataaatgaa tctccgcact taaaggggca tgatcagaaa ggtgtcgcca
     2821 tgtccgcagc ggcaacgcgg ttggcgcgct ggcgctggca tttttgacgt agatcctgtc
     2881 cagtcgtagt agaggaaatt gcaccggaaa cgtgcgcgcc gggcgtccgt gggcgcgggt
     2941 aaaaatctca tccagtccgg cctgcacttt taacggatga ttagcttttt gccgccagtc
     3001 attgaaatca cccgccacca ataccggttc gccgtccggt agctcattca cccattcggc
     3061 gagcatcgca agctgcgcct gacggtgcgc ctcacgcagg cccagatgta cgcacatcac
     3121 atgaatcgct tttccggtca tcggtggcac aatgcggcag tagagcacgc cgcgcttttc
     3181 cgcaccatcg accgaaacat cgcgattctc ataatgttca atgggataac gcgacagtac
     3241 ggcgttgccg tgatgccctt ccgggtatac ggcattgcga ccgtaggcaa aatcgctcca
     3301 catcgtgtcg gcgagaaact cgtagtgcga ggtatcgggc cagttttcca catgcagcgg
     3361 atgaacttcg tgcgcgccca tcacttcctg caggcaaaca atatcggcgc tgacggtacg
     3421 caccgcgtcg cgaagttccg gcaaaatgaa gcgtcggtta aacgcggtaa aacctttgtg
     3481 aatgttgatg gtgagcacct tgaacgaaaa ttgttgtgtt tgatcgggca taattttcct
     3541 gtctttgcct cttatctcat tgaaatagtg tagtcggcgt cacaaaaagg tgcggtctta
     3601 cggaattttc cgtaaagttc ggtactctga gtaagtagag ataaattctt caggagagaa
     3661 gccatgaagt ggcaacaacg tgttcgtgtc gcaactggtc taagttgctg gcagattatg
     3721 ttgcatttac tggtagtggc gctgctggtg gtgggctgga tgagtaagac tctggttcac
     3781 gtcggcgtgg gattatgcgc actgtattgt gtcacggtag tgatgatgtt ggtgtttcag
     3841 cgcccccccg agcaacgctg gcgtgaggtg gcagacgtgc tggaagagct gaccacgacc
     3901 tggtattttg gcgcagcgct gattgtgctg tggctgttgt cccgtgtact ggaaaacaac
     3961 tttttgctgg caattgcagg gctggcaatc cttgccggcc cggcggtagt gtctttgctg
     4021 gcgaaagata agaagttaca tcaccttacg tctaaacatc gcgtacgccg ctgaccctgt
     4081 cgtggccgtt atcaccagta gcggccacaa actattccac acaatatcca gactcgcatc
     4141 cttcaaataa atctgcttgg taatgtccgt aaagtggcga atagggttaa tccacgtcag
     4201 gttttgcagc catactggca tgttttccac cggagaaacg taaccggaaa ggagaatggc
     4261 gggcatcata aaaacaaaca cgccgataaa cgcctgctgt tgtgttgaac agagtgatga
     4321 aatcaacaga ccgaatccca ccagcgataa accataaatc accatcgtaa agtagaacag
     4381 cgccagcgat ccggcgaagg ggatttgata cgcccagata ccaatcgcca gcacaatggt
     4441 ggcctggaag gtggcgacaa ttaacgccgg tacggctttg ccgatgaaga tctgccaggt
     4501 ggtgagcggc gaaaccagta gctgatcgag cgtaccttgt tcacgttcgc gggcgacgga
     4561 aagtgaagtg acgatcatta cgccaatagt ggtgatcatg gcgatcagcg acggcaccac
     4621 aaaccatttg tagtcgagat tcgggttata ccagttgcgt accaccagct cgctgttatt
     4681 aggtttcggt tttccttcca gcagctcctg ctgataattt ttgacgatct gttgcaggta
     4741 gttggcggca atttgcgcac tgttggagtt acgcccgtcg aggatcaact gcaacggcgc
     4801 ggtctggaag gtatccagtt tgcgcgagaa gtcagccggg aaacgcacca ggagtaacgc
     4861 cttttgtgtg tcgatggttg ggcggatctc ctgtgggctt ttcagcagca gcacatgagt
     4921 aaaggcgctg gcgcgggcaa aacgttgggt cagctccacc gaatgctcgc cgttatcttc
     4981 atcgtagatg gcgatggtgg cgttagtcac ttccagcgtg gcggcgaacg ggaacaggat
     5041 cacctgaata agcacaggta aaatcagaat cgcgcgggtt tgcggttcgc gcagcaacga
     5101 ctgcaactct ttgcggatta acgtccataa gcgatgaaac atgctcttct ccctaatcca
     5161 gccgacgttt ggttttcagc cacgtcaggc cgataaacat caccgccgaa gcgatcaaaa
     5221 acagcacgtt taccaccagc accaccggaa tattcccggc gaggaacagg ctttgcaggg
     5281 tgctgacgaa ataacgagcg ggaataatgt acgtcaccgc gcggatcacc gcgggcatac
     5341 tgtcgatctg aaaaataaag ccggaaagca taatcgacgg cagaaaagcc gcgttcagag
     5401 cgacctgagc ggcattgaac tggttgcggg taatcgtgga aatcagcagc cccatcccca
     5461 gggtactgag taaaaacagg ctggagataa aaaacagaat cagcagcgaa ccgcgatacg
     5521 gcacgccgag aataaacact gacaccagca tacacagcaa catcgccagc atcccgagaa
     5581 agtaataagg gatcagctta cacagcagca gttccgtgcg ggtaatctcc gtagagagca
     5641 gagcctccat ggtgccgcgt tcccattctc gcgccaccac cagcgaggtg agaatcgcgc
     5701 cgatgaccgt catgatgatg gtcaccgcac cggggataat gaagtgctgg ctaatcgccg
     5761 ccgggttaaa ccagtagcgg gtttgtacat caataagcgg ttcaaaagtc tgcccgttgt
     5821 cctccgctcg ctgcatttgc cagatctgcc agatcccttc gacatacccc tgcacaaagt
     5881 tagcggtatt cggctcactg ccgtcggtga tcacctgaat cggtgcggtg gcgttggcgc
     5941 gctccatctg ttccgcaaaa tccaccggaa taaccaccag accgcgaatt ttccccgcct
     6001 gcattttggc gatcagttcc tgacggttat cgctgatggt ggcgtcgatg tagggcgaac
     6061 cggtcatagt gtgggtgaaa tccagcgccg cttcgctacg ctgttccagt aaaatcccga
     6121 cccgcagctt gctggagtcg aggttaatgc cgtaaccaaa aataaacagc agtagcagtg
     6181 ggatcactac cgcaatcagc cagctactcg gatcgcgaac gatctgccgc gtctctttaa
     6241 cgcacagcgc ccgcacgcga cgccaggaaa ggatcgggtt actcattgct atgctcctta
     6301 tcccagtcgt ggatcaactg aataaaggct tgctccatgg tgggatcggg ttgctcatcg
     6361 ttagccgact gtgctttcaa atcgtccggc gtaccgctgg cgattaattt cccacggtac
     6421 accaggccga tgcggtcgca atattccgct tcatccataa agtgggtggt gaccatcacc
     6481 gtgacgcctt tctctaccat gctgttgatg tgcagccaaa attcacggcg ggtgagggga
     6541 tcaacgccgg acgtcggttc gtcgagaaac agaatgtccg gttcatgcat cagcgaacag
     6601 gccagcgcca gccgctgttt aaaacctaat ggcagttcat cggtggcgtg ggaggcgata
     6661 cttttcaggc cgaatgcctc gctcatgcgg gagatttttt cgttctgcgc ccgaccgcgt
     6721 aagccataca caccagagaa aaagcgtaaa ttctgttcga ccgtcaggtt gccgtagagc
     6781 gaaaattttt gcgccatata gccgagatgc tggcgtgctt taccggaact ctctttcaga
     6841 tccatcccca gcaccagcgc ctgaccagag gtcggcacca gcaaaccgca catcatctta
     6901 aaggtggtcg atttacccgc gccgtttggc cccagcaaac caaaaatctc cccacgttta
     6961 acggcaaagt tgacgtgatc ggtggcggca aaatcaccaa atttcttggt cagttctttc
     7021 gcttcgatca ccgtctcgcc gggtgtgccg tctaccgtat gcaatattgc acccagcggc
     7081 gattctgagg ttccggcacc gcccagcaaa tcaataaacg catcttcaaa gcgcggtgta
     7141 gtttcgttga tattgatttc cggcatcccg tcggcatggc gaatatcgtc tggtgtggcc
     7201 tcttttttga ggatcagacg taccgatttc ccctgaatca tgccgtcgct gacctgtggc
     7261 agtttcaagg cgcgttgcaa tagtttgcgg ttgccctcgt gtggactggt catcagaaag
     7321 ctgcgtccgg ccatggtttg tgtcagggct tttggttccc cctgatacag caactcgcct
     7381 tcgttcatca gcagcacgtc acggcactgc tcggcttcgt cgagatacga ggtactccag
     7441 aggattaaca tcccttcgcc tgccagctca tgcaccatct gccacagttc gcgccgtgag
     7501 atagggtcaa cgccgacgcc gggttcatcg agcagcaaca ctttcggttc gcccaccagg
     7561 gtacaggcca gaccgagttt ttgtttcatc ccaccggaga gcttgcccgc caggcgtccg
     7621 gtaaacggcc caagagacgt aaactccagc aggcgagcaa aagtttgctt acgtgcctcg
     7681 ccggtgacgc tgcgcaaatc cgcgtacaga ttgaggttct ccatcaccgt gagatcttca
     7741 tacagaccaa atttctgcgg catatagccg agcacggcgt gcagtgcgcc gtcgtttttg
     7801 attggatcaa agccaatcac cgtggcattg ccgctgtcgg gtttcagtaa tcccgccagc
     7861 atccgcatta gcgtggtttt acctgcacca tccggcccca ccagccccgt cacataaccg
     7921 gcgtgaatgg tacaatcgag cggcgcgacg gcgggcttgt ccatgcccgg aaagcgtttt
     7981 tccaggccgt tcagcgtgat aacggcatca ttcatgtcct gcctcgttac cgaattgcac
     8041 cgtcaccggc attccctggc gtaacgcatc atcggcgtcg gtcaccacaa tacgcaggcg
     8101 atagacgagg tcggtacgca gatccggtgt ttcgacggtt ttcggggtaa attcagcagt
     8161 cggcgaaacg aaaccaatct gcccgtgata cggcttgtcc gggcgaccat cggtataaag
     8221 cagcactttg cgccccggct gggcctggtc aagattccgt tcatcaacat aagcgcgcac
     8281 ccacaccgga cgcgttagtg aaacggtaaa caccgtgcca ccttcattga ggaccgtgcc
     8341 tggctccacc gcgcgcgtta acagcgtgcc atcagacggg gctatcaacg ttgagtcctg
     8401 caaattcaac tccgcctgcg ccagttgcgc ctgcgcctgt tcgaggctgg ctttcgcctg
     8461 ggcgatgtcc tgttcacggt taccggaacg gtactgacgc aatttatcct gtgctgattt
     8521 cagcgttgcc tgcgcctggt cgcgcgagga gcgggcattt tccaggtcat ttgccgaaat
     8581 ggtgcggctt ttccacaacc cttgctggcg gttgtagaag ttctgcgcat agtcataggc
     8641 ggcctgcgcc tgttttacgg ctgcggcggc ctgagcgatt tcttcatcgc gatacccggc
     8701 aagtatcagg tcatactgcg cctgcgccac cgaaacaccc gctttcgcct gcatcagggc
     8761 aatctcatac ggcttgtgat ccagttcgcc cagcacctgg cccgctttga tagcatcacc
     8821 ttcgtccacc gccagcgatt caacgcgccc cccaacacgg aaactaagat tcaccgtacg
     8881 aatatccacg ttgccataca gcgtcaggcc gttatcctgg cggctttgat accaccagta
     8941 gcctccggca accacggcgg caagtaccac taccgccagt ccgatcacga caggtttttt
     9001 catcactaca aactcctttg cgataatcct tgcaaaatca ggtcgatatg acaggtcacc
     9061 gtctggttga tcagttcggt cttttcttca tcgaacgcgg tccagccggt acgtaacaga
     9121 attgtttctt tgccaagacg gaacgccaga atctcaccaa tcaacgcatg ggtatgaagg
     9181 atcatgcggg tgtcattggc gtcgctgccg gtccaggcgg caatcaggcg tgtcaggtgg
     9241 ctgtgtagcg gactaatcac ctgttcgtgc accaggtggt aggctgccgt gggagagagc
     9301 tgctcacggg agataaactt gctgaggttg acggtgtcat cctgggtgag cagcttaatc
     9361 atgttcctgc aggcgcgaag gatcagttca cggatggcag cccgatcagg ctgtggttgg
     9421 gcgaacaagc gttcggcttc ctcggcatgc ggacggaact gctcgccaat aaaatcggca
     9481 atccactggg cgcaggcgag gtacaaatct tctttcgaac cgaagtagta ggtgatggca
     9541 gcgatattct gcccggcctg ggcggctatc tcgcgggtgg tggcgttcat tccatattca
     9601 ccaaactgcg ccagtgcggc agcaatcagc tgttttttcg cctgttcacc cttgattgtc
     9661 atggcaggat tattcatcgc acagtccatt cttaatcaaa tgattgatta agattatgac
     9721 tccatatggg agttgtccag tatggctaag aattttagct acgccagtca cagggataat
     9781 ttatgcgctg cgtcacaaaa actgctacac tccgctccct catgacattg tggtttttgt
     9841 cattttcctt ttcagtatct ccctgaaaac tacaccggta acggtcgggg cggttcggag
     9901 tagttatgtc tttcgattct ttgggtttaa gccctgatat cctgcgcgcc gttgccgagc
     9961 agggttaccg tgaacccacc cctattcagc agcaggcgat ccctgcggtg ctggaaggcc
    10021 gcgacctgat ggctagcgcc cagaccggta ccggtaaaac agcgggcttt acgctgccgc
    10081 tgttgcaaca cctgatcact cgccagccgc acgccaaagg gcgtcgtccg gtacgtgcgc
    10141 tcattcttac cccgacccgt gaactggctg cgcagattgg cgaaaacgtc cgtgattaca
    10201 gcaaatattt gaacattcgt tcgctggtgg tgtttggcgg tgtcagcatt aacccgcaaa
    10261 tgatgaagct gcgcggcggc gttgatgttc tggtggcaac accgggacgt ttgctggacc
    10321 tggaacatca aaatgcagtg aagctggatc aggttgaaat cctcgtcctc gatgaagctg
    10381 accgcatgct cgacatgggc tttatccacg atatccgtcg cgtgttaaca aaactgccag
    10441 cgaaacgcca gaacctgctg ttctctgcga ccttctctga cgatattaaa gccctggcgg
    10501 aaaaactgtt gcacaacccg ctggaaatcg aagtggcacg ccgcaatacc gcgtctgatc
    10561 aggtgaccca gcacgttcac tttgtcgata agaaacgcaa acgcgaattg ctgtcgcaca
    10621 tgattggtaa agggaactgg cagcaggtgc tggtgtttac ccgtaccaaa cacggcgcta
    10681 accatctggc tgagcagctc aataaagatg gcatccgtag tgcggcgatc cacgggaata
    10741 aatcgcaagg tgcgcgtacc cgtgcgctgg ctgattttaa atcgggtgac attcgcgtac
    10801 tggtggcgac cgatatcgct gcgcgcggtc tggatatcga agagttgccg cacgtcgtca
    10861 actatgaact gccaaacgta ccggaagatt atgtccaccg tatcgggcgt accggtcgtg
    10921 cggctgctac cggtgaagcg ttgtcgctgg tgtgtgttga tgaacacaaa ctgctgcgtg
    10981 atatcgaaaa actgctgaaa aaagagatcc cgcgcattgc gattccgggc tatgagccgg
    11041 acccgtcaat caaagccgaa ccgatccaga acggtcgcca gcaacgtggc ggcggcggtc
    11101 gtgggcaagg tggtggtcgc ggtcaacagc aaccacgccg tggggaaggt ggcgcaaaat
    11161 ctgcaagcgc gaaacctgca gaaaaaccgt ctcgccgcct cggcgatgcc aaaccggcag
    11221 gcgaacaaca acgtcgccgc cgtccgcgta aacctgccgc tgcgcagtaa tcttttatgc
    11281 cgggctatgc ccggcatcag gctgatgaac aaacgcaaaa ctgtctgatg cgctacgctt
    11341 atcaggccta cgtgaactct gcaatatatt gaatttgcat gcttttgtag gccggataag
    11401 gcgtttacgc cgcatccggc atttcacaac aagcacttgt cagcaatttg agaacacggg
    11461 aaaaaattta tctgttttac cgcccataag ccaccgagac gttacttctc tatagccaat
    11521 tgttcgcgca actccattaa aaggtagccc agtcgattct tgcccttacc attaccaccg
    11581 tctccccagt aagcatcgtt ttccgtatgc tcaaccagtt ttgcgggcgc ggttgccagc
    11641 aagagcgcac gcagttctgc atgctgttcg aatttagcgc gaagtgcttt tcgcatcacc
    11701 tgttctttga ccgactccca gtttttacgc agaggcttag aacgatcgcg ccccatgcgt
    11761 gcggcgacca taggggaaga aacccgacgg atctcttcgc ggtatttttc atcaaggaat
    11821 ttttgtgcct gaaaatagtg ttctgaggtg ggccaggttt tcccgtcaac cttgatgggc
    11881 caggcggcaa agttagaaaa atcaccgtag tcatcgctgg tgctgtagaa atttatgatg
    11941 gtgtcttgca tgacgtgttg gatcctttgt gctcgaacgg gcattaaacc gcattatgtt
    12001 ggtggttatt gcgagccgct ttccagaaac agaaaaacca ttacccctga aaaccgaaaa
    12061 atgccacaat attggctgtt tatacagtat ttcaggtttt ctcatggcat taaccgccgc
    12121 gcttaaagcg caaattgccg cctggtataa ggcgcttcag gaacagatcc ccgactttat
    12181 tccccgtgcg ccgcagcggc agatgattgc ggacgtcgcc aaaacgctgg ccggagaaga
    12241 agggcggcat ctggcgattg aagcccccac cggcgttggg aaaacgctct cctatttgat
    12301 ccccggcatc gccattgccc gcgaagagca aaaaacgctg gtggtgagta ccgccaacgt
    12361 ggcgttgcag gatcagattt acagcaaaga tttaccgctg ctgaaaaaga tcattcccga
    12421 tcttaaattc actgccgctt ttggtcgtgg gcgctacgtt tgcccgcgta atctgacggc
    12481 gcttgccagc actgagccca cgcaacagga tctgctggcg tttcttgacg acgaactgac
    12541 gccgaacaat caggaagagc aaaaacgttg tgcgaagctg aagggcgatc tcgacactta
    12601 taaatgggat ggtctgcgcg atcatactga tatcgctata gatgacgatc tctggcgtcg
    12661 tttaagtacc gacaaagcca gctgcctcaa ccgcaattgt tactactatc gtgaatgccc
    12721 gttttttgtc gctcgtcggg agattcagga agcggaagtg gtggtggcaa accatgcgct
    12781 ggtgatggcg gcgatggaaa gcgaagcggt attgcctgac ccgaaaaatt tactgctggt
    12841 gctggacgaa ggtcatcacc tgccagatgt ggcccgggat gcgctggaga tgagcgctga
    12901 aatcaccgcg ccctggtatc gactacagct ggacttgttc acgaaactgg tcgctacctg
    12961 catggagcag tttcgcccga agaccatccc accgctggcg atccctgaac gtttgaatgc
    13021 gcattgtgaa gagttgtatg agcttatcgc ctcgttaaac aacattctca atctctacat
    13081 gcctgccggg caggaggcag agcaccgttt tgcgatgggc gaactgccag atgaagtgct
    13141 ggagatctgc cagcggctgg caaaactcac cgagatgctg cgtggcctgg cggagttatt
    13201 tcttaacgat ttaagtgaga aaaccggcag ccatgacatt gtacgtctgc atcggttgat
    13261 tttgcagatg aaccgcgcgt tggggatgtt cgaggcgcaa agcaaactct ggcggctggc
    13321 ttcgctggcg caatcttccg gtgcaccggt gaccaaatgg gcgacgcggg aagagcgcga
    13381 agggcagcta cacctctggt ttcactgcgt gggaatacgt gtcagcgatc agctggaaag
    13441 gctgctgtgg cgcagtattc cgcacattat tgtcacctcc gcaaccttgc gttcgctgaa
    13501 cagtttttcg cgtttgcagg agatgagtgg tctgaaagag aaagcgggcg accgttttgt
    13561 ggcgctggat tcccccttta accactgcga acagggcaaa attgttattc cccggatgcg
    13621 cgttgagcct tccatcgaca acgaagagca gcatattgcc gaaatggcgg cctttttccg
    13681 taagcaggtg gagagcaaaa aacatctcgg tatgttggta ctgtttgcca gcggacgggc
    13741 gatgcagcgc tttctcgact atgtgacgga tttacgtctg atgttgctgg ttcagggcga
    13801 tcagccgcgt taccgtttag ttgaactgca ccgcaaacgc gtcgccaacg gtgagcgcag
    13861 cgtgctggtg ggcttacagt catttgccga agggcttgat ttgaaaggtg atctgctcag
    13921 ccaggtgcat atccacaaaa tcgcttttcc gcccatcgac agcccggtgg tgatcaccga
    13981 aggggaatgg ctgaaaagcc tcaaccgcta tccgtttgag gtgcaaagcc tgccgagcgc
    14041 ctcgtttaac ctgattcagc aggttgggcg actgattcga agccacggtt gctggggcga
    14101 agtggttatc tacgacaaac gcttgctgac caaaaattat ggcaagcgac tactggatgc
    14161 attaccggta tttccgatag agcaaccgga agtccctgaa ggtatagtta aaaagaaaga
    14221 aaaaacgaaa tccccacgcc gtcggcggcg ttaatgatgt gagtcaggta aggagtcgta
    14281 aatggactat cgcaaaatca ttaaagagat cgggcgcggg aaaaaccacg cgcgcgattt
    14341 agaccgggat actgcgcgcg gtctgtatgc tcatatgctc aatggtgaag tccctgacct
    14401 tgagttgggc ggcgtattga ttgcgctgcg tatcaaagga gaaggggaag cagagatgct
    14461 cggcttttac gaagccatgc aaaatcacac catcaagctg acgccgccag caggcaagcc
    14521 gatgccgatt gtcatcccca gttacaacgg cgcgcgtaaa caggccaacc tgacgccgtt
    14581 gttggcgatt ctcctgcaca aactcggttt tcctgtggtg gttcacgggg ttagcgaaga
    14641 tccaacccgc gtgctgactg aaaccatttt tgaattgatg ggtattacgc caacgttgca
    14701 cggcgggcag gcgcaggcga agctcgacga acatcaaccg gtgtttatgc cagtcggcgc
    14761 gttttgcccg ccgctggaaa aacaactggc gatgcgctgg cggatgggcg tgcgtaacag
    14821 tgcacatacc ctggcgaaac tggcgacgcc atttgccgaa ggtgaggcgc tgcgtctttc
    14881 cagcgtttcg catccggaat acattggacg cgtcgcgaag ttctttagcg atatcggcgg
    14941 gcgggcgcta ctaatgcatg gtactgaagg tgaagtgtat gctaatccgc agcgctgccc
    15001 gcagatcaat ctcattgacc gtgaagggat gcgggtgctg tatgaaaaac aggacactgc
    15061 tggtagcgag ttactgccac aagcaaaaga tccggaaacc acggcgcagt ggattgagcg
    15121 ttgccttgct ggcagcgaac cgattcccga atcgctgaaa atccagatgg cttgctgcct
    15181 ggtggctacg ggtgaagcgg caactatcag cgacggcctg gcgcgcgtta atcaggcatt
    15241 ttaattcttt tctcagccgg atgagccatg tcatccggct tttaccccac cgcaatatga
    15301 aattcctgca tctttattga ccttcccacg cccggcgtgc agcataaaaa tacaacaaac
    15361 acataacata aacaggagtt aaccatggaa agtggtcatc gctttgatgc tcagacgctg
    15421 cacagtttta ttcaggctgt atttcgtcag atgggtagcg aggaacaaga agcgaaatta
    15481 gttgccgatc atttaatcgc ggcaaacctg gcagggcatg attcacatgg tattggcatg
    15541 atcccaagct atgtacgctc ctggagtcag gggcacctgc aaattaacca tcatgccaaa
    15601 accgttaaag aggcgggggc ggcggtcacg ctcgatggcg atcgcgcatt tggtcaggtc
    15661 gcggcacatg aagcgatggc gctggggatt gagaaagcgc atcagcacgg tattgccgcc
    15721 gtggcgctac ataactcgca tcatatcggc cgtatcggtt actgggcgga gcagtgtgca
    15781 gcggcggggt ttgtctctat ccactttgtt agcgtggtcg gtattccaat ggtcgcgccg
    15841 ttccacggtc gcgacagccg ctttggcacc aatccgttct gtgtggtttt ccctcgtaaa
    15901 gataatttcc cgctgttgct tgattacgcc accagcgcca ttgcatttgg caaaacccgc
    15961 gtcgcctggc ataaaggcgt ccccgtgccg ccaggttgcc tgattgacgt taacggcgtg
    16021 ccgacgacca atccggcggt aatgcaggag tcgccgttgg gttcgctgtt gacctttgcc
    16081 gaacataaag gctacgccct tgcagcgatg tgtgaaattc ttggcggggc gctttccggc
    16141 ggtaaaacga cgcatcagga aacgttacaa accagtcccg atgccattct taactgcatg
    16201 accactatca tcatcaaccc ggaactcttc ggcgcgccgg attgtaacgc gcagaccgaa
    16261 gcctttgccg agtgggtgaa agcctcgccg catgatgatg ataagccgat tttgctaccg
    16321 ggcgagtggg aagtgaacac gcgtcgcgaa cggcagaagc aggggattcc actggatgcg
    16381 ggaagctggc aggccatttg tgatgcagcg cggcagattg gtatgccgga agagacgttg
    16441 caggctttct gtcagcagtt agccagctaa aaaaaagccc gtccagtggc ggacgggcaa
    16501 acaagggtaa cataggatca atgagggtta gagcatatgc gtctgtcggc aaacagacag
    16561 ggaaatactt gtgctggacg tagcgtaaac gcctgatccg tccaacggtt cggattttgt
    16621 aggcctgata agacgcggta gcgtcgcatc aggcatctgt tgccggatgc ggcgtaaacg
    16681 ccttatccgt cctacgaatt acttataaat taccgcagta ccgcttaatt tattgttgtt
    16741 ggtggcggaa gtgatgctgt atccactggc accggctgcc gcggctttct cagccagttt
    16801 cgcttccagg gcatcgaggg tggatgcgcc atcggcagaa accacgccga ttttattcat
    16861 gttctgtgcc tgggatgccg ttaccggttc cgccgcgaac acgccaaatg acagagttga
    16921 aagagccata gcagcaacaa cagtattgat agttttcata attaatctct cgcaggtgat
    16981 ttttgtataa gtgacgttgt ttcgtcgatg tgatgagtat cacgtttttt tacgagagat
    17041 aaaatcgaag agaattgacc gc
//
LOCUS       DAFNBJ010000104        16686 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000104 DAFNBJ010000000
VERSION     DAFNBJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.104"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            257..409
                     /gene="mokC"
                     /locus_tag="L2X49_003261"
     CDS             257..409
                     /gene="mokC"
                     /locus_tag="L2X49_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBN4798296.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(513..1643)
                     /gene="dnaJ"
                     /locus_tag="L2X49_003262"
     CDS             complement(513..1643)
                     /gene="dnaJ"
                     /locus_tag="L2X49_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBN4798297.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1732..3648)
                     /gene="dnaK"
                     /locus_tag="L2X49_003263"
     CDS             complement(1732..3648)
                     /gene="dnaK"
                     /locus_tag="L2X49_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBN4798298.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQARNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLIEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4025..4429
                     /locus_tag="L2X49_003264"
     CDS             4025..4429
                     /locus_tag="L2X49_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBN4798299.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4455..5168
                     /gene="msyB"
                     /locus_tag="L2X49_003265"
     CDS             4455..5168
                     /gene="msyB"
                     /locus_tag="L2X49_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBN4798300.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5317..5883
                     /gene="satP"
                     /locus_tag="L2X49_003266"
     CDS             5317..5883
                     /gene="satP"
                     /locus_tag="L2X49_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBN4798301.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5918..6505)
                     /gene="mog"
                     /locus_tag="L2X49_003267"
     CDS             complement(5918..6505)
                     /gene="mog"
                     /locus_tag="L2X49_003267"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBN4798302.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6620..7573)
                     /gene="tal"
                     /locus_tag="L2X49_003268"
     CDS             complement(6620..7573)
                     /gene="tal"
                     /locus_tag="L2X49_003268"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBN4798303.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7852..9282
                     /locus_tag="L2X49_003269"
     CDS             7852..9282
                     /locus_tag="L2X49_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBN4798304.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9352..10128
                     /gene="yaaA"
                     /locus_tag="L2X49_003270"
     CDS             9352..10128
                     /gene="yaaA"
                     /locus_tag="L2X49_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBN4798305.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10281..10577)
                     /locus_tag="L2X49_003271"
     CDS             complement(10281..10577)
                     /locus_tag="L2X49_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBN4798306.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10791..12077)
                     /gene="thrC"
                     /locus_tag="L2X49_003272"
     CDS             complement(10791..12077)
                     /gene="thrC"
                     /locus_tag="L2X49_003272"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBN4798307.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12078..13010)
                     /gene="thrB"
                     /locus_tag="L2X49_003273"
     CDS             complement(12078..13010)
                     /gene="thrB"
                     /locus_tag="L2X49_003273"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBN4798308.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13012..15474)
                     /gene="thrA"
                     /locus_tag="L2X49_003274"
     CDS             complement(13012..15474)
                     /gene="thrA"
                     /locus_tag="L2X49_003274"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBN4798309.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLTAAQPGFPLAQLKSFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGANRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15500..15619)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15555..15620)
                     /gene="thrL"
                     /locus_tag="L2X49_003275"
     CDS             complement(15555..15620)
                     /gene="thrL"
                     /locus_tag="L2X49_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBN4798310.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15834..16520)
                     /locus_tag="L2X49_003276"
     CDS             complement(15834..16520)
                     /locus_tag="L2X49_003276"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBN4798311.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3891 a         4427 c         4129 g         4239 t
ORIGIN      
        1 gttaaataat taaatcatta aaaaattacg ccataaaaat gcgaggcgtc ttcaggatta
       61 gaatcgataa aacagaatgg gttaaagaga gcgatataat agcggcgggt gcttgaggct
      121 gtctgtctca ggcattagct gaacggcaga tagagaaaag ccccgagtga tattttacca
      181 tcaacccgag gccccctata tgctgaacac atgtagagtg cctcttactg accgtaaggt
      241 caaggagaag agagcaatga agcagcataa ggcgatgatt gtcgccctga tcgtcatctg
      301 tatcaccgcc gtagtggcgg cgctggtaac gagaaaagac ctctgtgagg ttcacatccg
      361 aactggccag acggaggttg ctgttttcac ggcttacgaa tccgagtaag agcaacggcg
      421 gggagtgatc cccgccactt tacaggtgct cgcatatctt caacgcaccc tatttttacc
      481 caggcctgcc cacgggcagg cttttgggga ggttagcggg tcaggtcgtc aaaaaacttc
      541 ttcacaccat caaagaagct ctttgagcgc gggctgttgt gctcgccggt tgggccaccg
      601 aagctttctt gcagctcttg cagcagctgt ttctgcttct cgttcaggcc taccggtgtt
      661 tcgacgacaa cgcggcacag taaatcaccc tgtgcgccac cgcggacaga cttgacgcct
      721 ttaccgcgca tacggaacag cttaccagtc tgggtttcgc caggcacttt cagtttgacg
      781 cgaccatcaa gggtcggtac ttcgatttcg ccacccagcg ccgccatagc gaagttgatc
      841 gggacttcgc aatacaggtt gttgccttca cgctcgaaaa tcgggtgctg tttaacctga
      901 acctgaacgt acagatcgcc tgccggtgcg ccatgctcgc ccgcttcacc ttcgcccgca
      961 agacggatgc ggtctccagt gtccacccct gccgggattt taacggacag cgttttgctg
     1021 cgctcaacac gaccatgacc atgacatttg ttgcacggat ctttgatcag cgtaccgcga
     1081 ccctgacagt gtggacaggt ctgctgcacg gcaaagaaac cctgacgcat ctgcacctga
     1141 ccagaaccat gacaggtcgg acaggtctgc ggctgtgtac ctggttttgc accgctaccg
     1201 tggcaaacgt cacactcttc cagagtcgga atgcggatct ctttggtcac gccacgtaca
     1261 gcttcttcga gggtgagctc catgttatag cgtaaatcag caccgcgcgc cgcacgttga
     1321 cgaccacgtc cgccgccaaa aatatcgccg aaaacgtcac caaaaatatc gctgaagtct
     1381 gcgccgccgc caaaaccgcc gccgcccata ccaccttgct caaacgcagc atgaccatac
     1441 tgatcgtatg ccgcacgttt ttgcgagtcg gtcagaactt cataagcttc cttgatctct
     1501 ttaaatttcg cctcggcctc tttgtcaccc tggttacggt ccgggtggta tttcatggcc
     1561 aggcgtttgt aggccttttt gatttcacgc tcttccgctg ttttggaaac gcctaaaatc
     1621 tcgtaataat cttgcttagc catctttttt aaattgcccc tagatgaatg cacgggcgga
     1681 gaggaaattc cccttcgccc gtgtcagtat aattacccgt ttatagggcg attatttttt
     1741 gtctttgact tcttcaaatt cagcgtcgac aacatcgtca tctttcgcgt tgtttgcaga
     1801 agcatcagca ccggcagtct gctgctgggc atgttgctgc tgggcgattt caatcagttt
     1861 ctgggaaacc tgtgccagct cctgcatttt cgcttcgata gcggctttgt cttcaccttt
     1921 cagagcagtt tccagtgcag tcagtgcaga ctcgatagca gttttgtcgt cagccggcag
     1981 tttgtcgcct gcttcttcaa cctgcttacg ggtgctgtgc agcagatggt cgccctggtt
     2041 gcgagcctgt accagctctt caaacttacg gtcagcttcg gcgttagctt ctgcgtcgcg
     2101 taccattttc tggatttcat cttcgttcag accagaagaa gccttgatgg tgatcttctg
     2161 ctctttaccg ctgtttttat ctttcgcgga aacgtgcagg ataccgtcag catcgatatc
     2221 gaaggtaact tcgatctgcg gcatgccgcg cggtgccggg ttgataccat ccaggttgaa
     2281 ctgacccaga gatttgttat cagccgcacg tttacgttca ccctgcagca catggatggt
     2341 taccgcagac tggttgtctt cagcggtaga gaacacctgg ctgtgcttgg tcgggatagt
     2401 ggtgtttttc gcgatcagcg tcgtcatcac accgcccatg gtttcgatac ccagagacag
     2461 cggggtaacg tccagcagca gtacgtcttt cacgtcacca gtcagaacac caccctgaac
     2521 agcagcaccg attgctacag cttcgtccgg gttaacgtct ttacgcggct ctttaccaaa
     2581 gaactcagca actttcttct gaaccattgg catacgagtc tgaccaccaa cgaggataac
     2641 gtcgtcgata tcagatacgg acaggccagc gtcctgcagt gcaactttca gcggctcaat
     2701 ggaacggttt accagatctt caaccaggct ttccagtttc gcacgagtca ctttgatgtt
     2761 catgtgtttc ggaccggtcg catctgcagt gatgtacggc aggttaacgt cggtctgctg
     2821 agcggaagac agttcgattt tcgctttttc tgccgcttct ttcaggcgct gcattgccag
     2881 cggatcgttg cgcaggtcaa tgccctgatc tttcttgaat tcttcaacca ggtagttgat
     2941 cagacggctg tcgaagtctt caccacccag gtgggtatca ccgttggtag ccagaacttc
     3001 gaaggttttt tcgccgtcaa cttcgtcgat ttcgataata gaaatatcga aagtaccacc
     3061 acccaggtca taaaccgcga tagtacggtt gccagtacct ttgtccagac cgtaagccag
     3121 cgcagctgcg gtcggttcgt tgatgatacg ttttacttcc agaccagcga tacggcctgc
     3181 gtctttggtt gcctgacgct gagcatcgtt aaagtatgcc ggtacggtga taacagcttc
     3241 agttaccggt tcacccaagt aatcttcagc ggttttcttc atttttttca gcacttcagc
     3301 agaaatctgc ggcggtgcca ttttctggcc tttaacttcg acccatgcgt cgccgttatc
     3361 agcagcaata attttgaacg gcatgatgga aacatcacgc tgtacttctt cgtcctggaa
     3421 gcggcggcca atcaggcgtt taatcgcaaa cagggtgttt tgcgggttcg tcactgcctg
     3481 acgtttagcc ggctgaccaa ccagagtttc accatcctgg gtataggcaa taatagaagg
     3541 cgtggtgcga tcgccttcgg cgttctccag cacgcgagga gtggtgccat ccataatcgc
     3601 tacacaagag ttggtagtac ccaggtcgat accaattatt ttacccatct aaacgtctcc
     3661 actatatatt cggtcatcat gtggttgtga gtctgtaata ggggcgaaac gtctggtttc
     3721 aactgcccaa tttcattttt ttgcagactc actcactgcg gttgactact aaatggggtc
     3781 gtaacccacg tcatcaaggg ggagatgcaa aaattttttg tgcttttgcg cagaaatttc
     3841 gcgatatagc aggacaagac gtgaaactga cagcaatttt attcatataa agaatgaatc
     3901 gcagccaaga cattcatcaa aaaattgtaa taaaaagaaa acattacgca cctgaatctt
     3961 ctctttatca gcagtaaact agtgggtatc catcccccta cctctcccca ctaagagaat
     4021 ccttatgaaa tccgttttta cgatttccgc cagcctggcg attagtctga tgctgtgctg
     4081 cacggcgcag gcaaacgacc ataaaatcct cggcgtcatt gcgatgccac gtaacgaaac
     4141 caacgatctg gcgctaaaac ttcctgtttg ccgaattgtt aaacgcatac aactctctgc
     4201 cgaccatggc gatttacagt taagcggtgc atcggtttat ttcaaagccg cccgtagcgc
     4261 cagtcagagc ctgaatattc cttcagaaat aaaagaaggg caaaccactg actggatcaa
     4321 cattaacagc gataacgaca ataaacgctg cgtctcaaaa atcacctttt ccggtcatac
     4381 ggtgaactca tcggatatgg ccacgctgaa aattatcggc gacgactaac gccagaatat
     4441 agaagccaca aaaaatgaat gttaattacc tgaatgattc agatctggat tttctccagc
     4501 attgcagtga ggaacagttg gcaaatttcg cccgattgct cacccataat gaaaaaggca
     4561 aaactcgcct ctccagcgta ctgatgcgca acgaactgtt taaatcgatg gaagggcatc
     4621 ccgagcaaca tcgccgcaac tggcagctga ttgctggaga attacagcat tttggtggcg
     4681 atagtatcgc caacaaactg cgcggacacg gtaaattgta tcgggccatt ttgctcgatg
     4741 tttcaaaacg attgaagctg aaagccgaca aagagatgtc tacgtttgag attgagcaac
     4801 agttactgga acaatttctg cgtaacacct ggaagaatat ggacgaggaa cataaacagg
     4861 agtttctgca cgcggtcgat gcgagggtga atgagctgga agagctgctg ccgctgctga
     4921 tgaaagacaa attattggca aaaggcgtgt cgcatttgct ttccagccaa ctgacccgca
     4981 ttttacgcac ccacgcagca atgagcgtac ttgggcatgg tttgctgcgc ggcgcggggc
     5041 tgggaggccc tgtaggtgcg gcactaaatg gggttaaagc ggtcagcggc agcgcctatc
     5101 gcgtgacgat tccagccgta ctgcaaatcg cctgcctgcg ccggatggtt agcgccactc
     5161 aggtctgata ccggaaaccg gggaaaattt tcattttccc ctcaaaagat cgtagacact
     5221 gccccactgg ctgattatta tgccgcgccc tgaaaacact acagttattc agggaaatta
     5281 tttcaccatt cattcgatga tgatttttga ggaattatgg gcaacactaa gttggctaat
     5341 ccggcaccgc tgggcctgat gggcttcggc atgaccacca ttctgcttaa cctgcacaac
     5401 gtgggttatt tcgctctgga cggtattatt cttgccatgg gcattttcta cggcggcatc
     5461 gcgcaaattt ttgctggtct gctggagtac aaaaaaggca acactttcgg tttaaccgca
     5521 ttcacctctt acggttcttt ctggctgacg ctggttgcga ttctgctgat gccgaaactg
     5581 ggtctgaccg atgcgccaaa tgcacagttc cttggtgtct atctgggtct gtggggcgta
     5641 tttacgctgt ttatgttctt cggcacgctg aaaggcgcac gcgttctgca attcgttttc
     5701 tttagcctga ccgtgctgtt tgccctgctg gcgatcggta acattgccgg taacgccgca
     5761 atcatccact ttgccggctg gattgggctg atctgcggtg ccagcgcaat ctatctggcg
     5821 atgggtgaag tactgaacga gcagtttggt cgcaccgttc tgccgattgg tgaatcccac
     5881 taattgtttt gagatccccc cgctcggggg gattttttta ttcgctaacg tcgcgtcttg
     5941 cactcttcgg tctgaatgct gcaaccactt ccggtgccgt ttcaacgtat ggcccttcca
     6001 gcaactgaat gcagtacggt acgctggcaa aaataccgtg taccacaacg ttacccgcag
     6061 cgtccttcac accttccagc gtctctttaa tagacttcgg ctgaccgggt aagttaagga
     6121 tcagcgcctg tttgcgaatc acaccaacct gacgcgaaag gatcgcagtt ggtacaaaat
     6181 gcaggcttat ctggcgcatc tgttcaccaa agccaggcat ctcgcggtcc gctactgcca
     6241 gcgtcgcatc gggcgttacg tcacgacgcg ccgggccagt tccgcccgtg gtgagcacca
     6301 gatggcaact catttcatcc actagctcac acaacgtttg ctcgatgatc gcctgctcat
     6361 cggggattaa gcgggtttcc agttcaaacg gcgtggttag cgccgatgtc agccattctt
     6421 ccagcgcagg gatgccttta tcctgataaa cgccgctgga tgcgcgatca gagatggaaa
     6481 ctaagccaat acgtaaagta ttcatcaaga tttttccgtt taaacagtct cgttaaacag
     6541 aatgatacac tgcgaaggga gtgacagaca ggcttcagaa gaggtagcgt gaccgacttc
     6601 ccggtcacgc taagaatgat tacagcagat cgccgatcat tttttccagt ttttcctggt
     6661 caatagcaaa cttacggata ccttccgcca gtttatctac tgccattgga tcctggttgt
     6721 gctgccacag gaactcggac tcagtgatac gcgccggacg cgctttcact tcgccggtgt
     6781 aagacagttt acgttcgata gccccttcgc tctcagccag ctctttcagc agtgccggtg
     6841 cgatggtcag acggtcgcag cctgccagtt ccagaatttc gccgatgtta cggaagcttg
     6901 cgcccataac cacggtttca taaccgtgct ctttgtagta ctggtagatt tcagatacag
     6961 aaaccacgcc cggatcttct gccggagcgt actctttctt atcggtattc gctttgtacc
     7021 agtcaagaat acggccaaca aacggcgaga tcaggaacac gcccgcttcc gcacaagcac
     7081 gagcctgagc gaaggagaac agcagggtca ggttacagtt gatgccttct ttttccagct
     7141 gttctgcagc acggataccc tgccaggtag aagccagttt gatcagaata cgatcgttgc
     7201 taataccagc atcgttgtag agtttgatca ggcgttttgc tttcgcaatt gacgcttcag
     7261 tgtcatagga aagacgcgca tcaacttcag ttgagatacg gcccggaacc agtttcagga
     7321 tttccagacc aatatttact gccagtttgt cggtcgcgtc cacgatctgc tgcgcgcgat
     7381 cgttgctctg ctgtttcgcc caggcgacag catcatcaat caacttacgg tattccggaa
     7441 tctgcgctgc gttaagaatg agagaagggt tggttgtggc atcctgcggt tgatacagct
     7501 tcattgccgc gatgtcccca gtgtcggcca ctacggtggt gtactgacga agggaggtca
     7561 atttgtccgt catgatagta tttctcttta aacagcttgt tagggggatg taaccggtct
     7621 gccctgatga tatcacgacg caacgtcagc gcaaccgccg acaagacggt tatcgccagg
     7681 cgagactgtt tcggatttct gacactgcgt tgatatcgcc cgccattttt atacaaaacc
     7741 tcatgtatgc tacgcagaag ttatcaagta cctcgtagcg tatatacttc ttaaacaatt
     7801 ggtaacgttt acacaggaaa gtcatcgcga ccggcaataa gagggatatg catgccagat
     7861 tttttctcct ttattaacag cgtcctttgg ggatcggtaa tgatttacct gctcttcggc
     7921 gcaggttgtt ggttcacttt tcgcaccgga tttgtgcagt ttcgctacat ccgccagttt
     7981 ggcaaaagtc ttaaaaatag cattcatcca cagccaggcg gtttaacctc atttcagtca
     8041 ttgtgtacca gtcttgcggc gcgcgtgggt agcggcaacc tggccggcgt tgcgctggca
     8101 attaccgccg gtggacctgg agccgtcttt tggatgtggg ttgccgcgtt tatcggcatg
     8161 gcgacctcgt ttgccgaatg ttctctggca caactttata aagaacgtga cgtcaatgga
     8221 cagtttcgtg gcggaccggc atggtatatg gcgcgcgggc tggggatgcg ttggatgggc
     8281 gttctgttcg ccgtcttttt gctcatcgcc tatggcataa ttttcagcgg agttcaggcg
     8341 aacgccgttg cccgcgccct aagtttttct tttgattttc ctccgctggt gacaggcatt
     8401 attctcgctg tctttgctct gctggcaatc actcgcggtc ttcatggcgt cgcccggctc
     8461 atgcagggtt ttgtcccgtt gatggcgata atctgggtac tgaccagcct ggtgatttgc
     8521 gtaatgaata tcgggcaact tccccacgtc atttggtcta tttttgagag tgcttttggc
     8581 tggcaggaag cggcaggcgg cgcggcggga tataccttaa gccaggcgat tactaacggt
     8641 tttcagcgca gtatgttttc caatgaggcg ggaatgggtt ctacaccaaa cgcagcagcg
     8701 gcagcggcgt cctggcctcc gcatccggca gcgcaaggga ttgtccagat gattggcatt
     8761 tttatcgaca ccctggtcat ctgtacggca agcgccatgc tgatattact ggcgggtaac
     8821 ggcacaacct acatgccgct ggaaggtatt cagcttatcc agaaggcgat gcgggtgcta
     8881 atgggttcct ggggtgctga gtttgttacc ctcgtggtta ttctgtttgc cttcagctcc
     8941 atcgttgcca actacattta tgccgaaaac aatctcttct ttttacgcct gaacaaccct
     9001 aaagcgatct ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg caccttgcta
     9061 agtcttccgc tgatgtggca actggcagat atcataatgg cctgcatggc tattaccaat
     9121 ttgaccgcca ttttactgct ctcgcctgtg gttcatacca ttgccagtga ttatctacgc
     9181 cagcgtaaac tcggcgtgcg cccggtgttt gatccgttgc gttatccgga tatcggtcgc
     9241 cagctttctc cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact atcgatcaac
     9301 tcattctctt tttttgctaa agtcggcata aatttcctgc aaggactgga tatgctgatt
     9361 cttatttcac ctgcgaaaac gcttgattac caaagcccgt tgaccaccat gcgctatacg
     9421 ctgccggagc tgttagacaa ttcccagcag ttgatccatg aagcgcggaa actgacgcct
     9481 ccgcagatta gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc
     9541 tttcatgact ggcagccaga tttcacgccg gagaatgccc gccaggcgat tctggcgttt
     9601 aaaggtgatg tctacaccgg cttgcaggcc gaaaccttca gcgaagacga tttcgatttt
     9661 gcccaacagc atttgcgaat gctttccggc ttgtatggcg tactgcgccc gctcgattta
     9721 atgcagcctt atcgtctgga aatggggatc cgtcttgaga atgcccgagg gaaagatctg
     9781 tatcaattct ggggagatat catcaccaac aagctgaacg aggcgctcgc agcacaaggc
     9841 gataatgtgg tgattaatct ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa
     9901 ttgaatgccg agattatcaa gccggtgttc ctcgatgaga agaacggcaa atttaagata
     9961 atcagcttct acgctaagaa agcacgcggt ctgatgagtc gttttattat tgaaaatcgg
    10021 ctgaccaaac cagagcaact gactggtttt aatagcgaag gttacttctt tgatgaagct
    10081 tcctccagca atggcgaact ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa
    10141 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag catgcagatt caatatattg
    10201 cagaattcac gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg aatgccggat
    10261 tgttacccgg catttgtcat ttagcggtga tgtttgcctg gaccatgacc gccgtgatga
    10321 tcatgaggag ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg gtgccagcga
    10381 ttgcctcgcc attcataatg ttgtttccac cagccgtggt cgcgccagtg acctccatcc
    10441 cagtaatagc cacgattatc acgatcgcct atctgtaatt ttactgacgg gactaacgta
    10501 atttccgcag cctgtgctgc catgggagcg accagaacca gggaaagtgc gagtacgata
    10561 gattgcatct ttttcacagg ttactccttt tatcattgcc cgttatgggt cgatatccgg
    10621 agattacgcg atgagaacga cggttgttat tctccgcaat cctaatctct aagttaccgc
    10681 atttattgag aatttctcct ttttccctgt cgtttttctc cataatttct tcataaaaaa
    10741 gccgggctgc ataaaagcaa acccggcctg attgagataa tgaatagatt ttactgatga
    10801 ttcatcatca atttacgcaa cgcagcaaaa tcggcgggca gattatgtga aagcaagggt
    10861 aaatcagcac gttctgccag ctcttttggc agatccaacg tttcaccgag aatcgcttcc
    10921 acgctctctt taaatttcgc cggatgcgcg gtgccgagga acaagccata ttcgcctgga
    10981 ttcaactggt cacgcagcgc acgatacgct acggcagcgt gcggctccga ggtgtagccc
    11041 agttctttta actcacgcat tgtctgttgc gtggtttcat catccacggc tgcataaccc
    11101 agctctttca gttgccagat tttgcggcgg aacaactctt ccacacgcgg ccagttgttc
    11161 ggctgactca cgtccatcgc gttggataac gtcgcctgag tcgctttggg tgaccactga
    11221 ccgtcgtgca ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc
    11281 accggcagac cgagtgactt cgccagcaga cccgccgtca aatcgccgaa gtttccgctt
    11341 ggcaccgaga caaccagctg gttgcgcgct tcctgcggca gctgcgcaac agcttcaaag
    11401 tagtagcaaa tctgcgccag caaacggctg atgttaatcg agttagctga gtttaacccc
    11461 agcgccactt tcagttcttc atcatcaaac gcctgcttca ccagcgcctg acaggcatcg
    11521 aaatcgccgt cgatggcaac agtttcgata ttgccgccca acgtacagaa cagtttttct
    11581 tgcagtggac tgattttgcc tcgtggatag aggataacca ctttcacatt cggtaaaccg
    11641 tagaaagcat gagccactgc cgctccggta tcaccggagg tcgcggtcag aatggtcact
    11701 ggcttatcgc ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta
    11761 aatgccagcg ttggcccgtg gaacaattcc agacaaccga catcgctttc aacattggcg
    11821 accggagccg ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc ctgcgggatt
    11881 tcatcaccaa taaacgccga gaggatcttc gcactgcggg tgacaaaatc cagcttcagc
    11941 atctcatcaa tttcagtcag gctgaattcc ggcaggtcat gcggaaaaaa cagcccctga
    12001 tttttgccca acccctgggt tacggcttgc gcaaagctga cctgctcgtt gtgatctttc
    12061 agattgtaga gtttcattta gttttccagt actcgtgcgc ccgccgtatc cagccggcaa
    12121 atatgaacaa aaccttcctg attttgcagg tagttcttac ccaaccagtc ggcaacgcgc
    12181 tgggcggtat ccggcttgtc acatagagcg aacaaggtcg ggccggagcc ggagataccg
    12241 ctcgctaccg cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac
    12301 cgttcacggt agggttcagc gataacatct ttcatcagct tcgcggcaag ctcaggctga
    12361 cgggaatagc aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg
    12421 cggcgatact gcgccggtaa aatagccctg gcttctgccg tcgagacttt aatccccgga
    12481 tacgccagca cccacagcca ctcatcaaac cctggcactt gctggctgat gatgtcgttt
    12541 tcttcgatca tcaactgcat accaccaaga aaacacggtg ccacgttgtc gtaatgaatg
    12601 ctgccggaga ttcgtccttc cagctcgccc atcaaagcca gcaaacgagt gtcattaagc
    12661 ggcttgccac agtgttcatt catcgccatc agcgccgcga ccaccgaaca ggcgctggag
    12721 cctaagcccg aaccgattgg catattcttt tccagagtca tcgccactgg aatttgcttg
    12781 ccaagctcct ggcaaaaacg ctcccagcac tgataaacga tattttcccg tggttctgac
    12841 ggcagcttat cggcaaagcg tccgaggttg ttgagactga atgtctctgc cgcctcaacc
    12901 gtgactacat ctccgagcaa tgcaccatca acaggtgtca ccgccgcccc gagcacatca
    12961 aacccgacgc tcatattggc actggaagcc ggggcataaa ctttaaccat gtcagactcc
    13021 taacttccat gagagggtac gtagcagatc ggcaaagaca ccggcagctg taacgtcatt
    13081 gcccgcacca tatccgcgca gcaccaacgg cagcggctga taatagtggc tataaaaggc
    13141 cagggcgttt tcgccatttt tcactttgaa cagcggatca ttaccatcca cttcggcaat
    13201 cttcacgcgg caggcaccat cttcatcaat attgccaaca tagcgcaaaa cttttccttc
    13261 atcacgggcc ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat
    13321 aaaagcggca acatcaccct cagcgttaaa ctctgcgggc agcacaggtt caatttcaat
    13381 atccgccagc tccagttcac gtcccgtttc acgagcgaga atcaatagct tacgcgctac
    13441 atccatacca gaaagatcat ctcgcggatc cggttcggta taacccattt cccgcgccag
    13501 agtagtcgcc tcggagaaac tcatgccttc gtctaacttg ccgaagatat aagaaagcga
    13561 acctgaaaga atgccggaga acttcatcaa ttcatcacca gcattgagca gattttgcag
    13621 gttctcaata accggtaatc cagccccaac gttggtgtca tagaggaatt tacgccgcga
    13681 tttttccgcc gcatgacgca acagatggta gtaatccatc gacgaggtgt tggccttttt
    13741 gttcggcgtg acaacgtgga aaccttcgcg caagaagtcg gcatattgat ccgccactgc
    13801 ctggctggag gtgcagtcaa caatgaccgg gttcagcaga tgatattctt tcacgaggcg
    13861 aattaagcgc ccgagattaa acggctcttt ggcttgcgcc agttcttcct gccagttttc
    13921 cagatttagg ccatgcacat tggtgagcag tgccttcgag ttggcaacac cgcagacacg
    13981 taagtcgata tgtttattct tcagccagct ttgttgacgc ttcagttgct ccagcagcgc
    14041 accgccaacg ccaccgacgc caatcacaaa cacttcgata acctgatcgg tattgaacag
    14101 catctgatga gtaacgcgca cgccagtggt cgcatcatcg ttatttacca cgacagagat
    14161 tgagcgttca gaagatccct gagcaatggc gacaatgttg atattggcgc gggccagcgc
    14221 ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat
    14281 aatggccagc cgttccgtca ccgccagcgg ctccagtaag ccttctttca gttccaggta
    14341 gaactcttcc tgcattgccc gttcagcccg cacacagtcg ctttgcggaa cgcagaaact
    14401 gatactgtat tcggaagatg attgcgtaat cagcaccacg gaaatacggg cgcgtgacat
    14461 cgcggcaaag acgcgcgccg ccatgccgac catccctttc attcccggac cagaaacgct
    14521 gaacattgcc atgttattca gattggaaat gcccttgacc ggtaattcgt cttcatcacg
    14581 gttggcacca atgagcgtac ctggtgcttg aggatttccg gtatttttaa tcaggcaagg
    14641 gatctggaac tgggcgatgg gggtaatggt gcgggggtga agaactttag cgccgaagta
    14701 ggaaagctcc atcgcttcct ggtaggacat cgacttcaac aacctcgcat cgggcacctg
    14761 acgcgggtcg caggtataga ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg
    14821 taaacaggca gccagcaccg ccgcggagta gtcggaaccg ttgcgtccaa gtaccaccag
    14881 ttcgcctttt tcattaccgg cggtgaagcc tgccatcagc accatgtgat cagccggaat
    14941 acgacttgcc gcaatacggc gggtggactc agcaatatcg acggtagatt cgaggtaatg
    15001 ccccactgcc agtagttttt cgaccggatc gataacggta acgttgtgac cacgcgcttc
    15061 taacacgccg gccataatgg cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt
    15121 gatgctatcc gggcactgcc ccaacaaact aatgccatgc aggacatgtt ttatttgggc
    15181 aaattcctgg tcgacgaaag atttcagttg cgccagcggg aatcccggct gggcggcggt
    15241 gagtcccgtc agaagttcgg caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc
    15301 ctggccgcta atggtttttt caatcatcgc caccaggtgg ttggtgattt tggcgggggc
    15361 agagaggacg gtggccacct gcccctgcct ggcattgctt tccagaatat cggcaacccg
    15421 cagaaaacgt tctgcatttg ccactgatgt accgccgaac ttcaacactc gcatggttgt
    15481 tacctcgtta cctttggtcg aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt
    15541 gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg
    15601 gtggtgctaa tgcgtttcat ggatgttgtg tactctgtaa tttttatctg tctgtgcgct
    15661 atgcctatat tggttaaagt atttagtgac ctaagtcaat aaaattttaa tttactcacg
    15721 gcaggtaacc agttcagaag ctgctatcag acactctttt tttaatccac acagagacat
    15781 attgcccgtt gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt tttttatttg
    15841 gtgatatttt tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct
    15901 aaaagcccca ggcgttgttg taaccagtcg accagtttta tgtcatctgc cactgccaga
    15961 gtcgtcagca atgccatggc tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt
    16021 gtgtcacttt tcgccggttg ttgtattaat gttgctaatt gatagcaata gaccatcacc
    16081 gcctgcccca gattgagcga aggataatct gccaccatcg gcacaccagt aagaacgtca
    16141 gccaacgcta actcttcgtt agtcagcccg gaatcttcgc gaccaaacac cagcgcggca
    16201 tggctcatcc atgaagattt ttcctctaac agcggtacca gttctactgg cgtagcgtag
    16261 taatgatatt tcgcccgact gcgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac
    16321 gattcagcca gtgtcgggaa aactttaata ttatcaataa catcaccaga tccatgtgcg
    16381 acccagcggg tggctggctc caggtgtgcc tgactatcga caatccgcag ttcgctaaaa
    16441 cccatcgttt tcattgcccg cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc
    16501 agaataatcg ttatacgcat attgccactc ttcttgacca aataaccgcg aacagggtga
    16561 tcactgtcaa cttattacgc ggtgcgaatt tacaaatcct taacgtaagt cgcagaaaaa
    16621 gccctttact tagcttaaaa aaggctaaac tatttcctga ctgtactaac ggttgaattg
    16681 ttaaaa
//
LOCUS       DAFNBJ010000105        16675 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000105 DAFNBJ010000000
VERSION     DAFNBJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.105"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(90..242)
                     /locus_tag="L2X49_003277"
     CDS             complement(90..242)
                     /locus_tag="L2X49_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBN4798312.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(507..1241)
                     /gene="cysH"
                     /locus_tag="L2X49_003278"
     CDS             complement(507..1241)
                     /gene="cysH"
                     /locus_tag="L2X49_003278"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBN4798313.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMLEEETRFFGLKRECGLHEG"
     gene            complement(1315..3027)
                     /gene="cysI"
                     /locus_tag="L2X49_003279"
     CDS             complement(1315..3027)
                     /gene="cysI"
                     /locus_tag="L2X49_003279"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBN4798314.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3027..4826)
                     /gene="cysJ"
                     /locus_tag="L2X49_003280"
     CDS             complement(3027..4826)
                     /gene="cysJ"
                     /locus_tag="L2X49_003280"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBN4798315.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5145..5507
                     /gene="queD"
                     /locus_tag="L2X49_003281"
     CDS             5145..5507
                     /gene="queD"
                     /locus_tag="L2X49_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBN4798316.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5585..6856
                     /locus_tag="L2X49_003282"
     CDS             5585..6856
                     /locus_tag="L2X49_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBN4798317.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6847..7107
                     /locus_tag="L2X49_003283"
     CDS             6847..7107
                     /locus_tag="L2X49_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBN4798318.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLISACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7124..7699
                     /locus_tag="L2X49_003284"
     CDS             7124..7699
                     /locus_tag="L2X49_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HBN4798319.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7847..8707)
                     /locus_tag="L2X49_003285"
     CDS             complement(7847..8707)
                     /locus_tag="L2X49_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBN4798320.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLIIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8704..9483)
                     /locus_tag="L2X49_003286"
     CDS             complement(8704..9483)
                     /locus_tag="L2X49_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBN4798321.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWRDYLRQRMQP"
     gene            complement(9461..10870)
                     /locus_tag="L2X49_003287"
     CDS             complement(9461..10870)
                     /locus_tag="L2X49_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4798322.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10892..12346)
                     /locus_tag="L2X49_003288"
     CDS             complement(10892..12346)
                     /locus_tag="L2X49_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBN4798323.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12416..13201)
                     /locus_tag="L2X49_003289"
     CDS             complement(12416..13201)
                     /locus_tag="L2X49_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBN4798324.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAASCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13520..14797
                     /locus_tag="L2X49_003290"
     CDS             13520..14797
                     /locus_tag="L2X49_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4798325.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14824..16302
                     /locus_tag="L2X49_003291"
     CDS             14824..16302
                     /locus_tag="L2X49_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBN4798326.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNYFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3991 a         4374 c         4186 g         4124 t
ORIGIN      
        1 attcacataa aaaatataaa ttttaaaaaa gggtgcagga gaccaaacca tcacaattca
       61 gtacgcgggg ttttaccccc gcgtggtggc tacttcttcg attcgtaagc gagaactgcc
      121 ttaaactcca taccacgttc tctgatactc aactcacaca acgagtcacc taccatcagc
      181 gtgaatccca gtactgtaca acacagtacg atgattgcca caagggcata ttttgtcagc
      241 atgtcttgcc tccctgcaaa gaagagacta acattcgaat tgtttgggtt cgaacgtaag
      301 cctcaggttg atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt
      361 aacgccagac agcctcaagc acccgacgcc attctatacc tgataattct tccagcggcg
      421 ttcacgttta aaatcaggaa tactgctcgc aaaataaaaa cgcccccatc atttctgaca
      481 gaggcgttta atttatccgg caatatttac ccttcgtgca atccacattc cctttttaag
      541 ccaaagaaac gtgtttcttc ttccaacatg ccaggttccc atttacgggt tgtatgggta
      601 tcaccgaccg ataaatatcc ttcatcccat aatgggtgat atttcaggcc atgtttttgc
      661 aggtactgat aaatagttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca
      721 cgctgaattg ccagcaccgg taaattggcg cggctgccgg attgttcgcg gcgcaggcca
      781 gcaaaccacg tttgcgcatt cagttctttc agcgcccggt tcatcggttc gactttgttg
      841 atgtcattgt acttttcaat gccttcaacg ccctgttccc acagtttgcc gtagcgtgct
      901 tcctgccagg cggcgctttc ggtagcgcgg tacactttca ggttgagctt gagtttgtcc
      961 gttaactcgt caataaagcg gtaggtttcc gggaacagat agccggtgtc ggtgaggatc
     1021 accggaatat ccgggtgaat ctgattcacc agatgcaggc tcaccgccgc ctgaatgcca
     1081 aagctggaag aaagtacata ttcaccgggc agattatcca gcgcccaggc aacgcggcct
     1141 tcagcgtcca gtttttccag ttcggcgtta gtttctgcca gcgccagaat gcgatctacc
     1201 ttcggcagtt cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc
     1261 ttgcctgatg cgacgctcac gcgtcttatc aggcctacaa gaccgggctg atggttaatc
     1321 ccacaaatcg cgcgccggat cgagcaccgg gcgaatgatg cccgcacgca ccgtaaagtc
     1381 gccgaagcct tcacccgctt cgcgctcttt cgcccagcgc cctatcagtt catcaagcga
     1441 cgccaggatt tccggctcgg tgatgttttc tttatacatc cgtgggatac gtgtcccaat
     1501 gcggttgccg ccaagatgca gattgtagcg acccggtgct ttacccacca ggcccacttc
     1561 cgccagcatc gcgcgaccac aaccgttcgg gcagcctgtt acacgcatca cgatatgctc
     1621 atcgctgaca ccatgtttcg ccattaaatt atcgatgttg tcgataaaag acggcaggaa
     1681 acgctccgct tccgccatcg ccagcgggca agtcgggaat gacacgcagg ccatcgagtt
     1741 ttcacgctgc ggcgtgacgg cattcattaa accgctctct ttggcgatct tctcgatctt
     1801 cgctttctcg ctttccggta caccggcgat gatcagattc tggttcgccg taatgcggaa
     1861 atcgcctttg tggatcttcg cgatctccag caggccggtt ttcagcggac gccccggata
     1921 atcaaggatg cgaccatttt cgataaacag cgtcaggtgc cagttatcat caatgccctt
     1981 aacccagcca atacgatcgc ctcgtccggt gaactcatat ggacggatcg gttcaaattt
     2041 gatccccgcg cgacgctcca cttccgcttt aaacgtctca acccccacgc gctccagcgt
     2101 gtatttggtt ttggcatttt tacgatcggt tcggttaccc cagtcacgct gagttgtcac
     2161 gacggcttcg gccaccgcca gcgtatgctc cagcggcaga tagccaaact cgctcgccgt
     2221 gcgggcgtag gttttcttgt tgccgtgttc gatagaaagc ccaccgccca ccagcaggtt
     2281 gaagcctacc agcttgccgt tttcggcgat tgccacgaag ttcatgtcgt tggcatgcag
     2341 atcgatatcg ttctgcggcg ggatcactac tgtggttttg aacttacgcg gcaggtaggt
     2401 ctgaccaagg atcggttctt catcagtagt ggcgactttt tcctggtcga gccagatctc
     2461 cgcatacgcg cgggtacgag gcaacagatg ctcagaaatc ttcttcgccc actcgtacgc
     2521 ttccgcgtgc agctgcgact cgtaagggtt cgaggtgcag agtacgttac ggttcatgtc
     2581 gttagctgtc gccagcgcat caagaccgac cgagtgcagc atctggtgca ccggtttgac
     2641 gttctttttc agaatgccgt ggaactgaaa cgtctggcgg ttggtcaggc gaatgctgcc
     2701 atagatggtg ttttcaccgg caaatttgtc gatcgcctgc cactgtttag tggtaatcac
     2761 cccacccggc agacgacagc gaagcagcat cgcgtggcgc ggctccagct tctgttcagc
     2821 acgttcggcg cggatgtcgc ggtcatcctg ctgatacatg ccgtggaagc ggatcagcag
     2881 gaagttatcg cccttaaagc cgccggtcag accgtcgttt aaatcttccg caatggtgcc
     2941 gcgcaggtag ttgctttcaa gcttcatgcg ctcggcgtct gtcagttttc cttcgaccac
     3001 taaaggccct ggatgttttt cgctcattag tagacatctc gctgataacg gcgctctacg
     3061 cgcagctcac ttaaaaattc atccgccgct tcggtgtcca tgccaccaaa ttcggcaatc
     3121 acttccagaa gtgcctgctc aacgtctttc gccatgcgat tagcgtcgcc gcagacataa
     3181 atgtgggcac catcattgat ccagcgccac agctccgcgc cctgttcgcg cagtttgtct
     3241 tgtacgtaaa ctttttcttt ttggtcgcgc gaccaggcaa gatcgatacg tgtcagcacg
     3301 ccatctttga cgtagcgctg ccactccacc tggtacagga agtcttccgt aaagtgcgga
     3361 ttaccaaaga acagccagtt tttacctggc gcttcgtcgg cggcgcgttg ctgcataaag
     3421 gcgcggaacg gcgcaatacc ggtgcctggg ccaatcataa tcaccggggt ttctggattg
     3481 gctggtaggc ggaagttatc gttatgttcg ataaatacgc ggacttcgcc ctcttcttcc
     3541 acgcggtcag cgaggaagct ggaggcacca ccggcacggg cgcggccttc cacgtcgtaa
     3601 cgcaccacac caacggtgac gtgtacttcg ttctcgactt ccgcctgcga ggaggcaatg
     3661 gaatagagac gcggcgtcag cgggcgcagc agattaatta gcgcttcggc atcaagctgt
     3721 gccggggaga aacgcaccat gtcgacaatc ggcgtcgtcg cggcgtaatg ctgtaacttc
     3781 gctttatcgc ccaccagcgg cagcagtgtt tcgctgcggg taagcgtggc gtaattctca
     3841 acaatgttgg cggtgttgac ggtcagttcg aagtgccact gtagcgcttc gttcagaggc
     3901 aacgttttgc cctcgacggt gacaggttca tcgcctttca gccacagcag ttcgacaagt
     3961 tctttcacca gtgccggatc gttctgatac cagacgccca gcgcgtcacc cggctggtaa
     4021 cgcaggcccg agtcacctaa gtcaatttcg atatggcgaa cgtctttttc agagttacgc
     4081 ccggtaattt tctggttaac agagaggcta gccaccagcg gcgcgtcttt gctgtacggg
     4141 ctggtgtgga tttcatttac cgcgccagta gcgacggatt gcgaaggtgc cgcgacaggc
     4201 gcacgcgatt taagcgcatc aaccacgcgg gcgcgccact cgctggcagc agcctggtat
     4261 tcaacatcgg catcgacacg gtcgagcagg cgttcaccac ccagttccgc cagcttgctg
     4321 tcaaaatctt tcccggactg gcagaaaaat tcataagagc tatcgccgag gctaaacacg
     4381 gcaaatgcgg tgttttccag cttcggcgct tttttggaga acaggaactt atgcagcgcg
     4441 acggcttctt ccggcggttc cccttctcct tgcgttgacg tcaccacgat aagcagtttt
     4501 tcgctggcga tttgtttgaa tttatagtcg cccgcgttca ccagcttaac gttcagtttt
     4561 gccgctaata aatcatcacg taatgcttca gcaacacggc gcgcattgcc ggtttgcgag
     4621 gcggagataa tagttatacc cggcatttct gcggcgggag ctggcgtcgc tgcaagcgca
     4681 gcaggctgct gattgagcac gccccagaaa tagccagaaa cccaggcaag ctgggtggga
     4741 gttaaatcgg tcgtggccgc ctgaaggcgt accagttgct ccgggttcaa cggaagcaac
     4801 gcggaaggtg ggacctgtgt cgtcatgcgt cgttatgttc cagtaagcaa agctgtttct
     4861 gcgccctgtc agcgcccata aaacagaaga gaaggtaagg ttaacggggt gaacggtgtg
     4921 gattaaagac gggatagcga taactaataa ccaaatcgac taacctgttt tagcaatagt
     4981 ctttaacaac aaaatagatt aaccaaccta atgaaaaaca aatgaattta gccaatcatt
     5041 aagataaatc agcgattttg cgcaacaaag tcgttttaga taatgcgaaa aaacagcctt
     5101 tccggtactc tacggcggtt ttatcgtcct gtagagaaat tatgatgtcc accacgttat
     5161 ttaaagattt caccttcgaa gccgctcacc gcttaccaca cgtcccggaa gggcataaat
     5221 gtggtcgcct gcacgggcat tcctttatgg tgcgactgga aattaccggg gaagtcgatc
     5281 cgcatacggg ctggattatc gatttcgctg aactgaaagc agcgtttaag ccaacttatg
     5341 agcgcctcga tcaccattat ctcaatgata ttccaggcct ggaaaacccg accagcgaag
     5401 ttttagcaaa atggatttgg gatcaggtta aacccgttgt gccgctgtta agtgcggtga
     5461 tggtaaaaga aacctgcacc gctggttgta tctatcgcgg cgaatgataa gagtgtgtcg
     5521 gcggtcaatt tcccttaagt aacgctatgt tagggtgttg tgttctggat atctggggca
     5581 tgacatggaa gacgactgcg acattattat tattggtgcc ggtattgcag gcaccgcttg
     5641 cgcgttacgc tgcgcgcgag cgggtttatc cgttttgtta ctggaacgcg ctgaaatccc
     5701 cggcagcaaa aatctttccg gcgggcggtt atatacccat gcactcgcgg aactcctccc
     5761 tcagtttcat ctgaccgcgc ctcttgaacg atgcatcact cacgaaagcc tttccctgtt
     5821 aacgccggat ggcgcaacga cgttttccag cttacagccc ggcggtgaat cctggagtgt
     5881 attacgtgca cggttcgatc cgtggctggt tgccgaagcc gaaaaagaag gtgtcgaatg
     5941 catccctggt gcgacggtgg atgcactgta tgaagaaaac ggcagggtgt gtggtgtcat
     6001 ttgtggtgac gatattctcc gcgcccgtta tgtggtgctg gcagaaggtg ccaacagcgt
     6061 cctggctgag cgtcacgggt tagtgactcg tcctgctggc gaagcgatgg cgttggggat
     6121 caaagaagtg ctgtcgctgg aaacatccgc tattgaagaa cgttttcatc tggagaataa
     6181 cgaaggcgca gcgttgctgt tcagcggcgg gatctgtgat gacttacccg gcggcgcatt
     6241 tctttatact aatcaacaaa cgctctcgtt agggattgtt tgcccgctct cttcccttac
     6301 gcaaagccgt gttccggcaa gcgagctgct gactcgcttt aaagcgcatc cggcagtgcg
     6361 cccgcttatc aaaaacacgg aatcactgga gtatggtgcg catctggtgc cagaaggtgg
     6421 cttgcacagt atgccggtgc aatacgccgg taacggctgg ctgctggtgg gcgatgcgtt
     6481 gcgcagttgc gtcaataccg gaatttccgt gcgcggcatg gatatggcgc tgactggcgc
     6541 gcaggcggcg gcacaaacgc tgataagcgc ctgccagcac cgcgagccgc aaaatctgtt
     6601 tccgctttat catcacaacg tagagcgcag cctgctgtgg gatgttctac agcgttatca
     6661 gcatgttccg gcgcttttgc aacgcccggg atggtaccgt acgtggcctg cgttaatgca
     6721 ggatatttcc cgcgatttat gggatcaggg tgataaacct gttccaccgc tgcgccagtt
     6781 attctggcat catttacgtc gtcacggcct gtggcatctg gcgggcgatg ttatcaggag
     6841 tctgcgatgt ctgtagcccg taatctctgg cgcgttgctg atgcgccgca cattgttccg
     6901 gctgactccg ttgagcgcca gacggcagaa cggttgatta gcgcctgtcc ggcaggtctt
     6961 ttttcgctca caccggaagg tgacttacgt attgactatc gcagttgcct ggagtgtggc
     7021 acctgccgtt tgctgtgcga cgaatcaaca ctacaacagt ggcgctatcc gccttccgga
     7081 ttcggcatca cctaccgctt tggataaata ataaggataa tttatgcccc tcttacacct
     7141 gctccgccag aatccggtga ttgctgccgt taaagataat gccagcctgc aactggcaat
     7201 cgattctgaa tgccaattta tttccgtgtt gtacggcaat atctgcacca tcagtaatat
     7261 cgttaaaaag attaagaacg ccgggaaata tgcttttatt catgttgatt tgctggaagg
     7321 cgcgtcaaat aaagaagtcg ttattcagtt tttgaaactg gtgaccgagg cggacggcat
     7381 tatcagtacc aaagcctcaa tgttgaaagc ggcaagagcg gaaggttttt tctgtattca
     7441 tcgcctgttt attgttgatt caatttcgtt tcacaacatt gataagcaag ttgcgcaatc
     7501 gaatccggat tgtattgaga tcctgccagg ctgtatgccc aaagtgctgg gctgggtgac
     7561 agagaaaatc cgccaaccgc tgattgccgg tgggctggtg tgcgatgaag aagatgcgcg
     7621 taatgcgatt aacgcgggtg tcgtggcgct ttccaccacg aataccgggg tctggacgtt
     7681 agcgaaaaaa ttactttgac gggataatcg taaccaattg aatttggttt gatttgtagg
     7741 ccgcacgcca catccgacat tcagcgcctg atgcgacgct tgacgcgtct tatcaggcct
     7801 acaagtcccc aggccagaac cgtaggcagg atcatccggc aacacgttat tggcaatctg
     7861 cgtgaatgtt tgtcaccagt gcctccagca ccactttcca gtcatcaacc acgccaacat
     7921 ctgcctgtga aaataccgca gcgctggcgt catgattaat cgccaccaca aacttgctat
     7981 tgcgtacgcc cgccattaac gctgctgcgc cagaagcgcc aacaacaata cacacctcag
     8041 gtgccagcag atgtccggaa ataccaatca ctttttcagc atcaacgcca ccgttcatca
     8101 ctcgcgcccg actgtagccc acttcggctc ccagcttttc tgccagcata gcgatttcct
     8161 ggttatctgc ctctccgccc tgccccacca ccagcacccg tctggcctca gctaatgggt
     8221 cgcgagtaac atttttcaga tcctctgtgc taaccagcca gtccggaagc gcacccggga
     8281 caatgatcag ttgttgcatt ccagagggca acgtggcgtt tttcgctgct cccgcctgac
     8341 gcgccaggga aaggcatagc gggcgttttt ctgtttgcag cgtcgccgtt agcgcatttc
     8401 cccagtggga tttgcgcacg cttacggttg gaatatctaa cgaagtcacc tggcagatgc
     8461 ttgcaccatg taaacgccag gccagccggg tcgacagttc atcgccaaac gttcccggcg
     8521 gaaagagaac aacgtccgca ggcgttcgct gccactgttc caccaacgca tccagcacct
     8581 gttccgccac tacaggctga ggttcaatct gccagtgcgc cagggtacaa ccgctgaaat
     8641 cctgcgccgc cagccagctt gctattgcgg cattctcctg atttattgtc acaattgcga
     8701 tattcatggc tgcatccttt ggcgtaaata gtcccgccat agcttttgag ctttttctgc
     8761 taccgtttgc ccatcaatca gcgtcgctcc gcgtcgttgc tctgctcttg ccagttgcag
     8821 acactgcatc gctggcattt cggcagcaac agttttacgg ataatttctg ctttccccgc
     8881 agccattcgc tggcgcattc ccggtactgg caaggccact tcaccgcact gacgcacggc
     8941 aatcaccgca ggcaagcgaa cccggcaaca acgcaaccca tgttcagtac gctgttcgag
     9001 ggtgataaac agcgcgtcga gagtgaaacg ctccacctgg gtgaagcagg gccagcccag
     9061 catttccgcc agcaaaaatg gcgtttgccc attctgccct tcgctgcttt ggcagccggt
     9121 aattatcaga tccagcggat tctgatgctg ccattcggca atatggcgag cgacaaattc
     9181 cggcgcaaag cgcagatctg ccgccgtctc cagcaataca gcttcctcaa acccaagagc
     9241 catgagatag cgcagccagt gcaatgcccg ttcatccccc atgctcaacg cggttaaaga
     9301 catcggcgta ccgttttttc tctgcgccaa cagcagcgcg gcagcggcct gttcatcagc
     9361 accgagtaaa cttcgcagta gcgaaatatc cggtccgctt ttaccctgag ccgccgcctg
     9421 ccactctttt tccgccagca ttccggcatc cggttcggct ttaaacgcta acagaatgtt
     9481 cattcgcacc tcctacattt cccgccgcca ccagcgggag ggctttagtt tctggtgccc
     9541 atagccaggt cacaacaaaa ccaaccaaca acactgtcgc caggagcaat aaggtgactt
     9601 gcattcccca ctgcgccagc acccacggca gcaggccagt acttaccgcc gcgccaagtc
     9661 gactcatggc ggtggcaaaa ccgacgccca gcgagcgaat gtcagtagga aaactttccg
     9721 caggcaaaat gcccaccaga ttactgactg ccgaaatggt ggtgctgaag agaacaaaaa
     9781 gcagcagcgt taatgaactg ccggaaggca aacaggccat gactaccagc gttgccgcca
     9841 gcagcaaaaa acttcccagc aaaaatttgc gatgtgccag caggtgcgtc agaactaatc
     9901 ccagcagcgc gcccacaatt aacaacgcat taagcatcag gctggcagtc agcgcatctt
     9961 ccagaccaat agtctgggcg atagttggca gccaggtata aatcacaaac catgggatta
    10021 cgaggcagac aaagaagacg ctgttaaacg ccgtgcgccg ccagtaacgc gaagagaaca
    10081 aggttttgat gtgtttatgg gtcgccgtta ccacttcatc gcccagtaaa acatggggac
    10141 caaaatagcg atgcacgata gcgtgagctt ctgcaaaacg cccctggcgc agtagccagc
    10201 gtggtgattc tggcgttccc cagcgtaata acgtaatcaa caacgcgggc agagctgccg
    10261 atgccagtag ccagcgccag gcctccgggt tttcggaaat aaagtgatgt ccggcaatac
    10321 ttgccagcac atagcctacg gtccacacca cgctgaatgc gcccagcaaa ataccgcgat
    10381 ggcggcgcgg ggaaaattca gccagcaagg tgtgacctac tgaataatcg cctcccagac
    10441 caatgccaat caaaatgcgc agtccaataa gatgctctgg cgtggtggca aaaaattgta
    10501 aaaacgaagc aagcgtaatc agcaaaaagc tgaaggtgaa gattttttgc cgaccaatat
    10561 ggtcggagat ccacccaaga accaggctgc caaggaacaa accaaggagc gccgagccgc
    10621 cgatcattcc cgccataaac ggcgtcagtt gcatggcggg cgtaagctga ataatggcgt
    10681 aaccaatgac gccgagaaca taaccgtcgg tcaggtgtgc gccgaaagtg agcgcagcaa
    10741 tgcggcagtg aaaacggtta agcggtaaat catccattcg caccggtgaa gtgttcatct
    10801 ttaccccttc attgtccggt aataagaacg gcttctcgcc tgagaagccg aaaaggcaac
    10861 gccccgtcat cgcggaacgc tgcctgacac attatttttc aatcggatag atagtacccg
    10921 tattcataat gccattagga tcgaactgct ttttcagccc ttccagcaac gcccacgcgc
    10981 tgccgtgttc cagtttgctc cagtgaacgc gatgtttacc gataccgtgg tggtgcacca
    11041 tcgaaccgcc gaggcgaatg gtttcttcac agatgatctt gttgagcgga ttgtggtact
    11101 tgtcgatttc ctcttccggc ttacagtcaa cgacgttgta atcgtagacg aagtacatgt
    11161 tggtgccgtt ctgatagcta tgagaggaat gaccgcccag catggtgatg tcgtcggcgt
    11221 gcgggaactc agtacgaata cggttaataa cgctttcgta gatttcgtgg atgcagctcc
    11281 agcagccgga cacttcagtg gtaaagccca tgttgccggt tttgaggatc tgcacacgtt
    11341 cggcagccac tttatccggt ccccagttca ggttgttaaa ccaggtttcg atcagtttgc
    11401 tgtccacgcg ctggcactgc gggtagcggg caacgatttc cgcaatccct tcgcccgtcg
    11461 ccttcgcaat gcgaggattg ccttcagcca taaagatcag cacacattta ccgtcggcaa
    11521 aatgggtgaa gtgttgggtg ccatcttcag cgtcatacaa acgagcgatc gacggacgat
    11581 acccttccac catgacttca cgcaggatgt tgaagccggt tttcatgtct tccaggatat
    11641 agccgtagaa gaggttgttt tccggggtga atttaaagat tttcactgtt acttcagtga
    11701 tatagcaaag tgcaccttcg ttaccgatga tgatgtggcg aatgtccggg ccagccgcgc
    11761 ggcgtggcac gtttttaatg cgtgtgacgg tgccatcagc caatactgct tccagaccaa
    11821 cgaccatatc ttcgattgcg ccgtagagtg tggagaactg cccgatactg cgggttgcta
    11881 ccaggccgcc catctgcgcc agcggctttg actgcggaga atgccccgtg gtgtaacctt
    11941 tttcacgcaa cgcattttcc agcacctcca gcggaacgcc acattgcgcc gtcgcctgca
    12001 tattctcaat atcaatatta atgatttgat tcatggcgga gccgtcgagc accaccgagt
    12061 tttccacaac ggtttccagc ccaccttcag tggcggaagc gccggtacgc ggcacaccgt
    12121 taattttgtg cgcattcata aaattcagca cacgggagac ttgttctgtg gaaccgagtt
    12181 ttacgaccgc tgccggaatc ggcaaagtat aaataccatg aatatccgga aatttacgaa
    12241 aacggtcaat actgttcttc tttaatactg tttcatcggt aattacgcga tctgcaccaa
    12301 caatttcctt tagttggtcg acaatcgctg cgcgagataa agacatagta attccttcct
    12361 gataataaaa atggaataat cattaataag agaaaacaga aaccaccgtt atgggttagc
    12421 gcactaaata accgccatca accactaata aatgcccgtt gacataattc gatgccggac
    12481 ttgcgaggaa tacggctgcg cccattaaat cctgagtatc gccccaacgg tttgccggaa
    12541 tatgatcaag aacgcgctga ttggtttctg gattactgcg tgtcgccagc gtaatatctg
    12601 ttgcataata gccaggggcg ataccattta cctgaatatt atattgacct agttcatcac
    12661 aataagcttt ggtgaacccg gcaagagcat gtttagtggc agaatatgca ggtgaccatt
    12721 gtccacctaa gtaagagaac aatgaacaga tattaataat tttgccgctt ttctgcggga
    12781 tcataatttt tgcagcttca tagcttaact cgaatgcggc ggtcaggttc acatcaatca
    12841 tcggatccca gtcggcacga ccgaagtcca gcaccttatt cagcttacaa ataccggcat
    12901 tgttaaccag aatatcaact gtaccgaaac gctcacagct agcagcgata atcttctgcg
    12961 gcgcgccttc tgcggtgata tccacctgca tgaagtccac ctcaacaccc tgtttttcaa
    13021 tcatttcctt tgtttcgccg ttatctttga cgaaactagg aataaagata tttgcgccag
    13081 ctttggccaa cgccatggca aatgcctggc ctaaaccgct attcccaccg gtaacaattg
    13141 cggttttacc tttcagggag aaaaaatcca ttgagaacgc attgagagat tcgattgaca
    13201 tagttagctc caatttccgt aaggcaaaaa aaaagaaagg taacttcccc cgcgagaggg
    13261 aagttacctt tctcattatc tctggtaact gtagataatt tttagcataa cggcataact
    13321 gctaatgtga tcgtaatcac agtgtgatat tcgtattgtc aaatagtttt aaaaagcaga
    13381 agcgtgatta ctctcacaca acacctctgc tttccatgtt atttcttatt gcaattacta
    13441 gagacctgag aaaagtaatc cacctgaaag ggcggcttac ttttctcttt tttttgtcta
    13501 ttacaaatgg gaatacgata tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat
    13561 aattagtttt agcggcggcg ttagtttcga cctggcttat ttacgttata tttatcaaat
    13621 tcccatggcg aaatttatgg gattcagcaa taccgagata ggtttaataa tgagtacctt
    13681 tggtattgcg gctattattc tttatgcccc cagcggcgtt attgccgata aattttcaca
    13741 ccgcaaaatg attacttccg cgatgatcat taccggatta ctgggtctgt taatggcaac
    13801 gtatccaccg ctctgggtaa tgctctgtat tcaggtcgcc tttgcgataa caacgatttt
    13861 aatgctgtgg tcggtgtcga ttaaagccgc ctcgttgctg ggcgatcata gcgagcaagg
    13921 gaaaattatg ggctggatgg aagggctgcg cggcgtcggt gtaatgtcgc tggcggtgtt
    13981 taccatgtgg gtcttttctc gctttgcacc ggatgacagc accagcctga aaacggtcat
    14041 tatcatctat agtgtggttt acatcttgtt ggggattctg tgctggtttt ttgttagcga
    14101 taacaacaac ctgcgcagtg ccaataacga agaaaaacag tcattccagc ttagcgacat
    14161 cctggccgtt ttgcgcatca gcaccacctg gtattgcagc atggtgattt ttggtgtctt
    14221 caccatctac gccattctga gttactccac caactatctg accgaaatgt acggcatgtc
    14281 tctggtggcg gcgagctaca tggggattgt gatcaacaaa atcttccgcg cgctgtgcgg
    14341 cccacttggc ggcattatca ccacctacag taaagtgaaa tcccctaccc gcgtgattca
    14401 aatcctttcc atcatcggtt tgctggcgtt gacagccctg ctcgtcacga actctaaccc
    14461 gcaatcggtc gcgatgggga ttggcctgat tttactgctg ggattcacct gttacgcctc
    14521 acgcgggctg tactgggcct gccccggcga agcgagaaca ccgtcttaca ttatgggcac
    14581 cacggtaggt atttgttcgg tgattggatt cctgccggat gtcttcgttt acccgattat
    14641 cggccactgg caagacaccc tgcccgctgc agaagcctac cgcaatatgt ggctgatggg
    14701 catggcggcg ctcggcatgg tgattgtctt tacctttttg ctgttccaaa aaattcgtac
    14761 tgctgatagc gcccccgcaa tggctagcag caagtaagcc aggaaaatta caggagaaaa
    14821 atcatgtcga aaaaatacat catagggatt gatggcggaa gtcagagcac aaaagtggtg
    14881 atgtacgatc tggaaggtaa tgtggtttgc gaaggtaaag gcttattaca gccgatgcac
    14941 acgccagatg ccgatactgc agaacatcct gacgacgatt tatgggcatc attatgtttt
    15001 gccggtcacg atttgatgag tcagtttgca gggaataaag aagatattgt cggtattggt
    15061 ctgggatcca tccgttgctg ccgtgcgtta ttgaaagccg atggcacgcc agctgcgccg
    15121 ttgattagct ggcaggatgc acgcgttaca cgcccttacg aacatacgaa tcctgacgtg
    15181 gcgtatgtca cctctttttc gggttatctg acgcatcgct taaccggcga gtttaaagac
    15241 aatatcgcca actattttgg tcagtggccg gtggattata agagctgggc atggagcgaa
    15301 gatgctgcgg taatggataa gtttaatatc ccccgtcata tgctgtttga tgtgcaaatg
    15361 cctggcaccg tcctcggaca tatcacacca caagccgcac tggcgacaca tttcccggca
    15421 ggactgccgg ttgtttgtac caccagtgat aaaccggtag aagctctggg ggccggatta
    15481 ctggatgatg aaactgcggt aatttctctt ggcacttaca tcgcattgat gatgaacggc
    15541 aaagcactgc cgaaagatcc ggtggcgtac tggccgatta tgtcttctat tccgcaaaca
    15601 ttgctgtatg aaggttacgg tattcgcaaa ggtatgtgga cggtgagctg gctacgcgac
    15661 atgttaggcg agtcgttaat tcaggatgcc aaagcacagg atctttcacc ggaagatttg
    15721 ctcaacaaaa aagcttcttg tgtgccgcct ggctgtaatg gtctgatgac ggtgctggac
    15781 tggctgacca acccgtggga accctacaaa cgtgggatta tgatcggctt tgattccagc
    15841 atggattacg catggatata tcgttcgata ctggaaagcg tggcgctgac gctgaagaac
    15901 aattacgaca atatgtgtaa tgaaatgaat tactttgcaa agcatgtgat cattactggc
    15961 ggcggttcga acagcgatct gtttatgcag atttttgccg acgtgttcaa ccttccggca
    16021 cgacgtaacg ccattaacgg ttgtgcaagc ctgggggcag cgattaatac agcggtaggt
    16081 ctggggctat acccggatta cgcaacggct gtcgataaaa tggttcgcgt gaaagatatc
    16141 tttatgcctg ttgagagcaa tgccaaacgc tacgacgcga tgaataaagg cattttcaaa
    16201 gatttaacca aacacactga tgtgattctg aaaaaatcgt atgaagtgat gcatggggaa
    16261 ttggggaatg cggattcgat ccagagctgg tcgaatgcgt gaatgaggta ggccaggcgg
    16321 aaaactgccc ggcctgaaca tacctaaata gttatccccg ctgacgcggg gaacataagt
    16381 tcgacaactt aaaaaatgat aacaagtccg gtttatcccc gctgatgctg ggaacataat
    16441 aaaaacttta actagttcgg tttatccctg tttttacaag gaatattaac tacactcgat
    16501 gtctttagaa taaaagacaa tacgaatcgc actctatcaa ttttcactta aagtatgaac
    16561 tggatatctt tattcataag aactattcat caacagcgta tagaggcggt tatgtaaaac
    16621 cactcattag cctcaaaaca aagacaaatt tccagattta ttacttaatc aatga
//
LOCUS       DAFNBJ010000106        16173 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000106 DAFNBJ010000000
VERSION     DAFNBJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.106"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            77..808
                     /locus_tag="L2X49_003292"
     CDS             77..808
                     /locus_tag="L2X49_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HBN4798327.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGEKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            1061..3277
                     /gene="traD"
                     /locus_tag="L2X49_003293"
     CDS             1061..3277
                     /gene="traD"
                     /locus_tag="L2X49_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBN4798328.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTENPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPQQPQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQ
                     LPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            3277..8547
                     /gene="traI"
                     /locus_tag="L2X49_003294"
     CDS             3277..8547
                     /gene="traI"
                     /locus_tag="L2X49_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBN4798329.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMVREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKISGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMQKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIGLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDV
                     VAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            8567..9313
                     /gene="traX"
                     /locus_tag="L2X49_003295"
     CDS             8567..9313
                     /gene="traX"
                     /locus_tag="L2X49_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBN4798330.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQSWMFLVGRGAFPLFALVWGLNLSRHTHIRQEAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRTWWRSAETMLLLAMWLPFSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            9368..9928
                     /locus_tag="L2X49_003296"
     CDS             9368..9928
                     /locus_tag="L2X49_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBN4798331.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            10558..11235
                     /locus_tag="L2X49_003297"
     CDS             10558..11235
                     /locus_tag="L2X49_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798332.1"
                     /translation="MEENNTSTDKNTLTALIDYENCGSLKNISLEQYGELIIFVGPQQ
                     NVVVLPADSFPEGARITIRQVSGVSRNNVDFHLVLELGRISCCAAGKDKTYHIISSDK
                     GYDGVIRTLKEAGITCIRRDVPESIAAAGESGADKSNCLKQPSVPESDIVSRWAGKLQ
                     QIAGSGAGNMPVSVETFNNYLKSHMRDEWNKTRAVAIRNELVRRGVTEVREKKIIWKK
                     AKKENKA"
     gene            11889..12101
                     /locus_tag="L2X49_003298"
                     /pseudo
     CDS             11889..12101
                     /locus_tag="L2X49_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
     gene            12345..12806
                     /locus_tag="L2X49_003299"
     CDS             12345..12806
                     /locus_tag="L2X49_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBN4798333.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVFQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            12852..13061
                     /gene="hha"
                     /locus_tag="L2X49_003300"
     CDS             12852..13061
                     /gene="hha"
                     /locus_tag="L2X49_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBN4798334.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            13099..13689
                     /locus_tag="L2X49_003301"
     CDS             13099..13689
                     /locus_tag="L2X49_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBN4798335.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGECWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            13929..14183
                     /gene="repA"
                     /locus_tag="L2X49_003302"
     CDS             13929..14183
                     /gene="repA"
                     /locus_tag="L2X49_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBN4798336.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCM"
     gene            14421..14495
                     /gene="tap"
                     /locus_tag="L2X49_003303"
     CDS             14421..14495
                     /gene="tap"
                     /locus_tag="L2X49_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBN4798337.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            14488..15345
                     /gene="repA"
                     /locus_tag="L2X49_003304"
     CDS             14488..15345
                     /gene="repA"
                     /locus_tag="L2X49_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBN4798338.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTLALFAALDV
                     SEDAVAAARRSRVEWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT         4045 a         3864 c         4784 g         3480 t
ORIGIN      
        1 cgttataatc tctcagctat tgcctcagta ataggactgt tcaaaaaaga gtctgttagt
       61 taaaaggata ttttggatga aaacaaaaaa attgatgatg gttgcactgg tcagttccac
      121 tctggccctt tcagggtgtg gtgcgatgag cacagcaatc aagaagcgta accttgaggt
      181 gaagactcag atgagtgaga ccatctggct tgaacccgcc agcgaacgca cggtatttct
      241 gcagatcaaa aacacgtctg ataaagacat gagtgggctg cagggcaaaa ttgctgatgc
      301 tgtgaaagca aaaggatatc aggtggtgac ttctccggat aaagcctact actggattca
      361 ggcgaatgtg ctgaaggccg ataagatgga tctgcgggag tctcagggat ggctgaaccg
      421 tggttatgag ggcgcagcag ttggtgcagc gttaggtgcc ggtatcaccg gatataattc
      481 cagttctgcc ggtgccacac tcggtgtggg ccttgctgcc ggtctggtgg gtatggctgc
      541 agatgcgatg gtggaagatg tgaactatac catgatcacg gatgtgcaga ttgcagagcg
      601 tactaaggca acggtgacaa cggataatgt tgccgccctg cgtcagggca catcaggtga
      661 gaaaattcag accagtactg aaacaggtaa ccagcataaa taccagaccc gtgtggtctc
      721 aaatgcgaac aaggttaacc tgaaatttga agaggcgaag cctgttctcg aagaccagct
      781 ggccaaatca atcgcaaata ttctctgatt atcttccgga ggctggtctg accggcctcc
      841 tgactgacgc ctcgctttgc tcgttgtcca accccggaaa gaaataaaat aaattttatt
      901 tctccccggc gtttccgaaa taaatatgaa aacactgagc ggttgtgcaa attctttgtg
      961 cgaccgtgaa gtgttgccgg cgaagccgga atttaacggt tattaatctt ttcatcatta
     1021 ataatcagtc cgactgactt tttttcttcg gaatatcatc atgagtttta acgcaaagga
     1081 tatgacccag ggcggtcaga ttgcgtccat gcgtatccgc atgttcagcc agatcgccaa
     1141 tatcatgctt tactgcctgt ttattttttt ctggatactc gttggtctgg ttttatgggt
     1201 aaaaataagc tggcagacgt ttgtgaacgg ctgtatttac tggtggtgta ccacgctgga
     1261 aggcatgcgg gatttaatca agtcccagcc ggtctatgag atccagtatt acggcaaaac
     1321 cttccggatg aacgctgctc aggtactgca tgataaatat atgatctggt gcggagagca
     1381 gctatggtcc gcattcgttc tggctacagt tgtggcactg gttatttgcc tgattacctt
     1441 ctttgttgtc tcctggattc tggggcgtca gggtaaacaa cagagcgaaa atgaagtcac
     1501 aggtggtcgt cagctgacgg agaacccgaa agacgttgcc cggatgctga aaaaagacgg
     1561 caaggactcc gatatccgga ttggcgacct gccgattatc cgggattccg aaatccagaa
     1621 cttctgtctg cacggcacgg tcggggccgg taagtcggaa gttatccgtc gtctggccaa
     1681 ctacgcccgt cagcgtggag atatggtggt gatttatgac cgttcagggg aatttgttaa
     1741 aagttactat gatccctcca tcgataaaat cctgaatccg ctggatgccc gctgtgccgc
     1801 ctgggatttg tggaaggagt gcctgacaca gccggatttt gataataccg cgaacacgct
     1861 gatcccgatg ggcacaaaag aggacccgtt ctggcagggt tcaggacgta ccatttttgc
     1921 ggaagcggcg tacctgatgc gtaatgaccc caaccgcagc tacagcaaac tggtggacac
     1981 actgctttcc atcaaaatcg aaaaactgcg taccttcctg cgtaattcac cggcggccaa
     2041 cctggtggaa gagaaaattg agaaaacggc gatttccatc cgtgctgtgc tgaccaacta
     2101 cgtgaaagcc atccgttacc tgcaggggat tgagcataac ggtgagtctt ttaccatccg
     2161 tgactggatg cggggtgtcc gggaagatca gaaaaacggc tggctgttta tctcgtcgaa
     2221 tgccgacacc catgcctccc tgaagccggt gatctccatg tggctgtcca ttgccattcg
     2281 tggtctgctg gcaatgggag aaaaccgtaa ccgtcgtgtg tggtttttct gtgatgagct
     2341 acccacgtta cacaaactgc cggacctggt ggagatcctg ccggaagccc gtaagttcgg
     2401 tggctgttat gtgtttggta tccagtccta tgcccagctg gaagatatct acggtgagaa
     2461 agcggctgcc acgctgtttg acgtcatgaa cacccgtgcc tttttccgtt ctcccagcca
     2521 taagattgca gagttcgctg ccggtgagat tggtgagaaa gagcacctga aagccagcga
     2581 gcagtactcc tacggtgctg atccggtacg tgacggggta tcgaccggta aggatatgga
     2641 gcgccagacg ctggtcagtt attccgacat tcagtctctg ccggatttga cctgttatgt
     2701 caccctgccc ggaccgtatc cggcagtaaa actctcactg aaatatcagg cacgtccgaa
     2761 ggttgcccct gagtttattc cgcgtgacat caacccggaa atggagaacc gcctgagcgc
     2821 cgtacttgcc gcaagggaag cagaaggtcg tcagatggcc agcctcttcg aaccggatgt
     2881 gccggaggtt gtttccggag aagacgtgac tcaggctgaa cagccgcaac agccgcaaca
     2941 gccgcaacag ccgcaacagc cgcaacagcc gcaacagccg caacagccgc aacagccggt
     3001 gtctcctgcc atcaacgata agaagtcaga ttcaggtgtg aatgttccgg caggggggat
     3061 cgagcaggag ctgaaaatga aaccggaaga agagatggaa cagcaactgc cacccgggat
     3121 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcaac aggaaaatca
     3181 tccggacatc cagcagcaga tgcagcgtcg tgaagaggtg aacattaatg tgcaccggga
     3241 gcgcggggag gatgttgagc cgggagatga tttctgatga tgagtatcgc gcaggtcaga
     3301 tcggccggaa gtgccggtaa ctattatacc gacaaggata attactatgt gctgggcagc
     3361 atgggagaac gctgggccgg caggggggct gaacagctgg gactgcaggg cagtgtcgat
     3421 aaggatgttt ttacccgtct tctggagggc aggctgccgg acggagcgga tctaagccgc
     3481 atgcaggatg gcagtaacag gcatcgtccc ggctacgatc tgaccttctc cgcccccaaa
     3541 agtgtctcca tgatggccat gttaggtggc gataagcgcc tgattgatgc acataaccag
     3601 gccgtggact ttgctgttcg tcaggtggag gcgctggcct ccacacgggt gatgacggac
     3661 ggacagtcag aaacggtgct gaccggtaat ctggtgatgg cactgtttaa ccacgacacc
     3721 agtcgcgatc aggaaccaca gttacacacg catgcggtgg tggccaatgt cacgcagcat
     3781 aacggcgaat ggaagacact gagcagtgac aaagtgggga aaacggggtt cattgagaat
     3841 gtgtacgcta atcagattgc ctttggcagg ctctaccggg aaaaactgaa agagcaggtt
     3901 gaggcgctgg gctatgaaac cgaagtggtg ggtaagcacg gtatgtggga gatgccgggc
     3961 gtaccggtgg aggccttttc cggacgctca caggcaatcc gggaggccgt cggggaagat
     4021 gcctcgctga aatcccggga tgtggcggca ctggatacgc gaaaatccaa acagcacgtc
     4081 gatcctgaag tcagaatggc tgaatggatg cagacgctga aggaaaccgg gttcgacatc
     4141 cgggcatatc gtgacgcggc ggatcagcgt gcggagaccc gcacgcaggc gcccggggct
     4201 gtttcacagg aggggccgga tgtgcagcag gcggtgacac aggccattgc cggattaagt
     4261 gaacgaaaag tgcagtttac gtacacggac gtgctggcca gaacggtcgg catactgccg
     4321 ccggaaaatg gtgtgattga gcgggcccgc gccggtatcg atgaggccat cagccgtgag
     4381 cagctcattc ccctcgatcg tgagaagggg ctgttcacgt ccgggattca tgtgctcgat
     4441 gagctgtcag tccgggcact cagtcgtgac atcatgaaac agaaccgggt gaccgtacat
     4501 ccggagaaaa gtgtcccccg gacggctggt tacagcgatg cggtcagtgt tctggcacag
     4561 gaccgtccgt cgctggccat tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg
     4621 gtggctgaac tggtcatgat ggtccgggag caggggcggg aggtgcagat tatcgctgct
     4681 gaccgtcgct cgcagatgaa cctgaagcag gatgaacggc tgtccggtga gctgataacc
     4741 ggacgtcgtc agctgctgga aggcatggcc ttcacgccgg gcagcactgt tatcgttgac
     4801 cagggcgaaa aactctccct gaaagagacg ttaaccctgc tggacggtgc cgcacgtcat
     4861 aacgtacagg tcctgataac cgacagcggg cagcgaaccg gtacaggcag tgcgctgatg
     4921 gccatgaagg atgccggggt gaacacatat cgctggcagg ggggagaaca gcgaccggcc
     4981 accatcatca gtgaaccgga ccgtaatgtc cgctatgccc ggctggccgg agattttgcg
     5041 gccagcgtga aagccggaga agagagcgtg gcacaggtca gcggagtacg ggaacaggcc
     5101 atactgacac aggccattcg cagtgagctg aaaacacagg gcgtgctcgg acaccaggag
     5161 gtgaccatga cggcactgtc accggtctgg ctggacagcc ggagccgtta tctgcgggat
     5221 atgtaccgtc cggggatggt gatggagcag tggaacccgg agacacgcag tcatgaccgc
     5281 tatgttatcg accgggtgac ggcgcagagt cacagcctga ccctgcggga tgcgcagggt
     5341 gaaacgcagg tggtgcgtat ttcctccctg gacagcagct ggtcgctgtt ccggccggaa
     5401 aaaatgccgg tggcagacgg cgagcgactg agggtgacag ggaaaatttc cggactccgc
     5461 gtctccggcg gtgaccgcct gcaggtggca tccgtcagtg aagatgcgat gacggttgtt
     5521 gtgccggggc gggctgaacc ggcctccctg cctgtgagcg attcaccatt cacggcactg
     5581 aagctggaga acggctgggt ggaaacgccc gggcattccg tcagcgacag tgcgaaggtt
     5641 tttgcctccg tcacacagat ggcaatggac aacgccaccc tgaacggtct ggcccgcagc
     5701 ggtcgtgatg tccggctgta ttcctcactg gatgaaaccc gtactgcgga aaaacttgcc
     5761 cgccatccct cctttacggt ggtttctgag cagataaagg cgcgtgccgg tgagacattg
     5821 ctggaaaccg ctatcagtct gcagaaaacc gggcttcaca cgccggcaca gcaggctatt
     5881 catctggcgc ttcctgtggt ggaaagtaaa aacctggcct tcagcatggt ggacctgctg
     5941 acagaggcga agtcgtttgc tgcagaagga accagtttta ctgaactggg aggggaaatc
     6001 aatgcgcaga taaaacgcgg tgatttactg tatgtggatg tggcaaaagg ctatggcaca
     6061 ggcctgctgg tttcccgtgc gtcgtatgag gcagaaaaga gcattcttcg ccatattctc
     6121 gaaggtaagg aggcggtcac gccactgatg gagagagtac ctggcgaact catggagaaa
     6181 ctgacatcag gacagcgtgc cgctacccgc atgatactgg aaacgtccga ccgtttcacg
     6241 gtggtgcagg gctatgccgg tgtgggtaag accacacagt tccgggcggt gatgtcagcc
     6301 gtgaacatgc tgccggagag tgaacgtccc cgtgtcgtgg ggctggggcc cacgcaccgt
     6361 gcggtcggtg agatgcgcag cgccggcgtg gatgcacaga cgctggcgtc ctttctgcat
     6421 gacacgcagc tgctgcagcg cagcggagaa acgccgaatt tcagcaacac gctgttcctg
     6481 ctcgatgaga gctctatggt gggcaatacc gacatggcac gggcatacgc cctgattgcg
     6541 gccggtggcg gtcgtgccgt ggccagtggt gacacggacc agttgcaggc catcgcgccc
     6601 ggtcagcctt tccgtctcca gcagacgcgc agtgctgccg atgtggccat catgaaggag
     6661 attgtgcgcc agacgccgga gctgcgggag gcggtataca gcctgattaa ccgggatgtg
     6721 gaaagggcac tgtccgggct tgagagtgtg aaaccgtctc aggtgccacg tctggagggc
     6781 gcatgggcac cggagcactc cgtgacggag ttcagtcaca gccaggaagc gaaactggca
     6841 gaagcgcagc agaaggcgat gcagaaaggc gaggctttcc cggatgtccc catgacactg
     6901 tatgaagcca ttgtccgcga ctataccggc agaacaccgg aagcacggga gcagacgctg
     6961 attgtcacgc acctgaatga ggaccggcgc gtgctgaaca gcatgattca tgatgcacgg
     7021 gaaaaggccg gtgagctggg aaaagagcag gtcatggtgc ctgtcctgaa cacagcgaat
     7081 atacgcgacg gggagctgcg tcgtctctcc acctgggaga cacatcggga cgcacttgcc
     7141 ctggtggata atgtgtatca ccggattgcc ggtatcagca aggatgacgg gctgataacc
     7201 ctgcaggatg cggaaggtaa cacgcggctg atttcgcccc gggaggcggt ggctgaaggc
     7261 gtcacactgt acactccgga caccatccgg gtgggaaccg gtgaccggat gcgcttcacg
     7321 aagagtgacc gggagcgcgg ttatgtggcc aacagcgtct ggacggtgac agcggtttcc
     7381 ggtgacagtg tcacgctgtc ggacggacag cagacccggg tgattcgccc cggtcaggag
     7441 cgggcagagc aacatattgg cctggcctat gccatcaccg cccacggtgc gcagggggca
     7501 agtgaaacct ttgccattgc gcttgaaggc acggaaggta accggaaaca gatggccggc
     7561 tttgagtcag cctacgtggc cctgtcgcgt atgaagcagc atgtgcaggt gtataccgat
     7621 aaccgtcagg gctggacgga tgccattaac aatgccgtac agaaaggaac tgcccacgat
     7681 gtgctagagc cgaaatcgga ccgggaggtc atgaatgcag agcggctgtt cagtacggcg
     7741 cgggagctgc gggacgtggt ggccggccgt gccgttctcc gtcaggcagg gctggccggg
     7801 ggagacagtc ctgcacggtt tattgcaccc gggcgcaaat atccgcagcc gtatgtggca
     7861 ctgccggctt ttgaccgtaa cggcaagtca gctggtatct ggctgaaccc gctgaccacg
     7921 gatgacggaa acgggctgcg gggcttcagt ggtgaaggcc gggtgaaagg cagcggggat
     7981 gcgcagttcg tggccctgca gggcagccgt aacggagaga gcctgctggc tgataatatg
     8041 caggatggtg tccggattgc ccgtgataat cctgacagcg gtgtggtggt aagaatcgcc
     8101 ggtgaaggtc gtccgtggaa tcccggtacc atcaccggtg gtcgcgtgtg gggggatatc
     8161 ccggacaaca gcgttcagcc gggagccgga aatggcgaac cggtcacggc agaggtactg
     8221 gcacagcggc aggctgaaga ggccatccgc cgtgaaacgg aacgccgtgc ggatgaaatt
     8281 gtccgtaaaa tggcagagaa caaacctgac ctgccggacg gcaaaacaga gcaggctgtc
     8341 agggatattg ccgggctgga gcgtgaccgg tctgccatat ctgaacggga agccgcgctg
     8401 ccggagagtg tactgcgtga accacaacgg gtgcgggaag cggtccggga ggttgcccgg
     8461 gaaaatctgc tgcaggagcg actgcagcag atggagcggg atatggttcg tgacctgcag
     8521 aaagagaaaa ccctgggcgg agactgacac aggaagataa acgctgatga caacggataa
     8581 cacgaacaca acacgtaatg attcactggc tgcccggacc gatacctggt tgcagtcgtt
     8641 gctggtctgg tcacccggac agcgggatat catcaaaacg gtggcactgg tgctgatggt
     8701 tctggaccat gcaaaccgca ttctgcatct ggaccagtcg tggatgtttc tggtcgggcg
     8761 tggggccttt ccgctgtttg ccctggtgtg ggggctgaat ctgtcccgtc atacgcatat
     8821 ccggcaggag gccattaacc ggctgtgggg atgggcggtg attgcccagt tcgcatatta
     8881 tctggccggc tttccctggt atgaggggaa tatcctgttt gcctttgcag ttgcggcaca
     8941 ggtgctgaca tggtgtgaaa cgcgcacctg gtggcgttcc gcagagacca tgttgcttct
     9001 ggcaatgtgg ttgcctttct ccggcaccag ttacggcatt gccgggctgc tgatgctggc
     9061 agtcagccac cggctgtacc gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg
     9121 cctgctggct gttattccgg cgcttaacct tgccaccagt gatgcagcgg cggtggccgg
     9181 tcttgtgatg acggtgctga ccgttggtct ggtgtcgtgt gcaggaaaat cattaccccg
     9241 tttctggccc ggggattttt tcccggtatt ctacgcctgt catctggctg tgctgggcgt
     9301 tctggcgctg tgacgggtgt ggtatctttg accgtaacag gatgattcgt cagaggcaga
     9361 acacagcatg acagagcaga agcgaccggt actgacactg aagcggaaaa cggaagggga
     9421 gacgcctgtc cgcagccgga aaaccatcat caatgtcacc acgccaccaa agtggaaggt
     9481 gaaaaagcag aagctggccg agaaagcagc ccgggaagca gagctggcgg caaaaaaagc
     9541 gcaggccaga caggcgctgt ccatttatct gaacctgccc acactggatg aggccgtgaa
     9601 caccctgaag ccctggtggc cgggattgtt tgacggtgac acgccccggc ttctggcctg
     9661 cggtatccgg gacgtgttac tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa
     9721 aaaactgcgc cgggcgctga aggccatcac ccgttcagaa agctatctgt gtgccatgaa
     9781 agccggtgcc tgccggtatg acacggaagg gtatgtgacg gagcatattt ctcaggagga
     9841 ggaagcgtat gcggcagcgc gtctggataa aatccgccgc cagaaccgga taaaggcaga
     9901 acttcaggcc gtgcttgatg agaaataaaa aaaagcctcc ctcagggagg ggaggcaggg
     9961 aaataaggct taaaaggaaa taacttccgt acccgaaaga aagccattcc actgaactat
    10021 ttatcacaat aatactgaca actaaagggg gaaggaaaat gaacggaaca gggcgtgatt
    10081 cgcaaaactc cggggtatgg cgctaccggc aggtcggatg ccgggctgtc cggctggaag
    10141 tcaggggggg actggccggg agccgctgag gcatggctac gggctgcgca gtctgctccc
    10201 cggacagact ggcaacagtt tgcacaacag cgggctgaac ggtgccggag gcgctgccgg
    10261 ggaaaggtgt aacgaaaaaa cagccttcgt tgctccaggg aaccggaagg ctgaagtatg
    10321 catgagattt ttctttttta tatctggcct ggcagtatga ccagtcagca ttcattatca
    10381 caggattatt gctgtattaa gtaatagcgt gctgttttgt gattgatttc ataaaacaag
    10441 acgtcgttta atatacgcct gaatattcac gagggaagtc tttcttttta tatggtgttc
    10501 ccggaaataa aatattttgt gttattgctc acccggttat gaataaaagg taaaaacatg
    10561 gaagaaaata atacgagtac agataaaaac accctgacag cccttattga ttatgaaaac
    10621 tgtggcagcc tgaaaaatat ttcccttgag cagtatgggg aactgattat ttttgtcggg
    10681 ccacagcaga acgtcgttgt tcttcctgca gactcttttc ctgaaggtgc gagaatcact
    10741 atcaggcagg tgagtggtgt gtccaggaat aatgtcgatt ttcatcttgt tctggagctg
    10801 gggcggataa gctgttgtgc agcagggaaa gataaaacat accacatcat ttcatcggat
    10861 aagggatatg acggtgttat caggacgctg aaagaagccg gtattacctg catccgtcgt
    10921 gatgtaccag aaagtattgc tgctgccggt gagtctggtg cagacaaaag taactgcctg
    10981 aaacaacctt ctgttcctga aagtgatatc gtcagccgtt gggcaggaaa gctgcagcag
    11041 atagccggca gcggagccgg taatatgcct gtgtccgtcg aaacatttaa taattattta
    11101 aagtcacaca tgcgtgacga atggaacaaa acccgggcag tggcgataag gaatgaactg
    11161 gttcgtcggg gagtcacaga agtcagggag aaaaagataa tctggaaaaa ggcgaaaaag
    11221 gagaataaag catgaggtat cgcagctgta ctcagataaa aacagccccc gttgcggttg
    11281 cggagcgaag ggggctgaaa agaggaatta aggatgattg ttattatggt gaaccagaag
    11341 gttatccctg tctgtaagct gaatcggact aaacgacata atacctgtaa catattttcc
    11401 tggcaggaca agcaggaaca ataaaatgtg tgatttaatt caaaaaatgt gcgcggcaaa
    11461 gaaaaaccgg ggaattgctt ccccggtccg gtccatttag ccagtaccct gagcaggatc
    11521 tgatatgagc attattcact ttgccagaca tggtgaaaaa agtgaatata tggaggagaa
    11581 aaacatcgcc tgaatcacag attcgggtgc tcagcgcgta agaagacggg aaagaaaagc
    11641 gcctttccgt ttcagcatcg ggaataatca ctgaaacatc agcggtatat gtggtgtgta
    11701 tgaatatccc ggcagcatta attcttctgt gaggaaatgc attgataaga aacagaataa
    11761 gtcggtggta aggaaaatga atttattaat tcagtatccg tttatcagat aaaaaaggga
    11821 aagagtatgg tgaaactgat tatttttatt atcacaataa tactgacaac taaagggggg
    11881 acaggaaaat gaatggattc agaaacagtt cacggaacgg tcaggtctga cgttaccaga
    11941 gtgccggaag ccgggccgtt attctggaag tcagtggacg ctggatggaa gcggcagaag
    12001 catggggacg ggctgccggt gttgcacccc ggacagactg gcagcagttt gcccgaaaaa
    12061 gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaagc agccttcatt
    12121 actccaggga ttcagaaggc tgaataatgc atgagatttg ctttttttaa tatccggtcg
    12181 ggcagtatga ccggacagtt ctgagtagca cagagttttt tccgtattaa gtcgagattt
    12241 tatattttgt gatttatttc acaaaacaag gtgttattca gtgtgtgctg caatgttcag
    12301 gatgcctgaa atacaggtgc catttatctg atatggagaa taacatgagg aaatatattc
    12361 cactggtatt atttatcttt tcatggccgg tattgtctgc agatattcat ggacgggttg
    12421 ttcgtgtact ggacggtgat accatcgagg tcatggactc acggaaggcg gtgcggatca
    12481 ggctggtaaa tattgatgca ccggaaaaga aacaggatta tggccggtgg tcaacggaca
    12541 tgatgaaatc actggttgca ggaaaaacgg ttaccgtcac atattttcag cgagatcgtt
    12601 atggtcgtat tctcgggcag gtttatgcac cagacggaat gaatgttaat cagtttatgg
    12661 tccgggccgg ggctgcctgg gtatatgagc agtacaacac agatcccgtg ctgcctgtct
    12721 ttcagaacga agccagacaa caaaaacgtg ggctctggtc agatgctgat ccggttcccc
    12781 cctggatatg gagacaccgt aaataaagcg ctggcaatct tatcattcat ctgttctcag
    12841 cgggaataaa aatggcaaaa acaaaacagg aatggctgta tcagttacgg cgatgttcat
    12901 cagtcaatac gctggaaaaa attattcata aaaacagaga cagtttatca accagtgaac
    12961 gtgaatcatt taattcagca gcagaccacc ggttagcgga attaatcacc ggaaagttat
    13021 atgacaggat accaaaagag atatggaaat atgtaaggta ataattcagc ataattaatt
    13081 accaggagca gataaacaat gaagctgatt atttttattc ttattgttct gattattgca
    13141 gcgttactta tccggattat cctcagaagt gtaaatcagc attctccttt gctgatgcaa
    13201 ttgcatgcgg caggtatacg gaccggtgat gcagagcgaa tactgtccag cggtgaatgc
    13261 tggcaacgtc agaagacgct gctgacagaa agggaagtca gttttatgaa aggactgttc
    13321 agaattgtgg atatgaagcg gtggtatctg tgtccgcagg tacgggtcgc ggatatcgtc
    13381 cagctgaacg ggaatatccg gccacgatca cgccagtggt ggcagttatt caggatggtg
    13441 tctcagtggc atgttgatgt ggttatcgtt gagcggcgtt cgttcagtat tgttgcagca
    13501 gtagagctgg atgatgccag ccatttacga ccggaacgca gacgccggga tattcttctg
    13561 gaagaggttc tgaggcaggc tggtattccg ttgctcagaa gccacgatgc cagaaaactg
    13621 ctgcagatga ccggagaatg gctgaataca acaggggctg atcagcagtc cccggaacat
    13681 cgtagctgac gccttcgcgt tgctcagttg tccaaccccg gaaacgggaa aaagcaagtt
    13741 ttccccgctc ccggcgtttc cataactgaa aaccatacta tttcacagtt taaatcacat
    13801 taaacgacag taatccccgt tgatttgtgc gccaacacag atcttcgtca caattctcaa
    13861 gtcgctgatt tcaaaaaact gtagtatcct ctgcgaaacg atccctgttt gagtattgag
    13921 gaggcgagat gtcgcagaca gaaaatgcag tgacttcctc atctggcgca aaacgagcat
    13981 acagaaaggg gaatccgctt tctgatgcag agaaacaaag attatcagtg gcccgtaaaa
    14041 gagcttcgtt caaggaagta aaagtatttc ttgaaccaaa gtataaggcc atgctcatgc
    14101 aaatgtgtca tgaagatggc ctgactcagg ctgaagttct gaccgcactg ataaaaagtg
    14161 aagcgcaaaa acgatgcatg tgatgatggg cttacattct tgagtgttca gaagattagt
    14221 gctagattac tgatcgttta aggaattttg tggctggcca cgccgtaagg tggcaaggaa
    14281 ctggttctga tgtggattta caggagccag aaaagcaaaa accccgataa tcttcttcaa
    14341 ctttggcgag tacgaaaaga ttaccggggc ccacttaaac cgtatagcca acaattcagc
    14401 tatgcgggga gtatagttat atgcccggaa aagttcaaga cttctttctg tgctcgctcc
    14461 ttctgcgcat tgtaagtgca ggatggtgtg actgatcttc accaaacgta ttaccgccag
    14521 gtaaagaacc cgaatccggt gttcactccc cgtgaaggtg ccggaacgct gaagttctgc
    14581 gaaaaactga tggaaaaggc ggtgggcttc acctcccgtt ttgatttcgc cattcatgtg
    14641 gcgcatgccc gttcccgtgg tctgcgtcgg cgcatgccac cggtgctgcg tcgacgggct
    14701 attgatgcgc tgctgcaggg gctgtgtttc cactatgacc cgctggccaa ccgcgtccag
    14761 tgttccatca ccacactggc cattgagtgc ggactggcga cagagtccgg tgcaggaaaa
    14821 ctctccatca cccgtgccac ccgggccctg acgttcctgt cagagctggg actgattacc
    14881 taccagacgg aatatgaccc gcttatcggg tgctacattc cgaccgacat cacgttcaca
    14941 ctggctctgt ttgctgccct tgatgtgtct gaggatgcag tggcagctgc gcgccgcagt
    15001 cgtgttgaat gggaaaacaa acagcgcaaa aagcaggggc tggataccct gggtatggat
    15061 gagctgatag cgaaagcctg gcgttttgtg cgtgagcgtt tccgcagtta ccagacagag
    15121 cttaagtccc gtggaataaa acgtgcccgt gcgcgtcgtg atgcgaacag agaacgtcag
    15181 gatatcgtca ccctggtgaa acggcagctg acgcgtgaaa tctcggaagg acgcttcact
    15241 gctaatggtg aggcggtaaa acgcgaagtg gagcgtcgtg tgaaggagcg catgattctg
    15301 tcacgtaacc gcaattacag ccggctggcc acagcttctc cctgaaagtg atctcctcag
    15361 aataatccgg cctgcgccgg aggcatccgc acgcctgaag cccgccggtg cacaaaaaaa
    15421 cagcgtcgca tgcaaaaaac aatctcatca tccaccttct ggagcatccg attccccctg
    15481 tttttaatac aaaatacgcc tcagcgacgg ggaattttgc ttatccacat ttaactgcaa
    15541 gggacttccc cataaggtta caaccgttca tgtcataaag cgccagccgc cagtcttaca
    15601 gggtgcaatg tatcttttaa acacctgttt atatctcctt taaactactt aattacattc
    15661 atttaaaaag aaaacctatt cactgcctgt cctgtggaca gacagatatg cacctcccac
    15721 cgcaagcggc gggccccgac cggagccact ttagttacaa cacacaaaaa caacctccag
    15781 aaaaaccccg gtccagcgca gaaccgaaac cacaaagccc ctccctcata actgaaaagc
    15841 ggccccgccc cggcccaaag ggccggaaca gagtcgcttt taattatgaa tgttgtaact
    15901 acacagcatc atcgctgcca gtcttctggc tggaagttct cagtacacgc tcgtaagcgg
    15961 ccctgacggc ccgctaacgc ggagatacgc cccgacttcg ggtaaaccct cgtcgggacc
    16021 actccgaccg cgcacagaag ctctctcata gctgaaagcg ggtatggtct ggcagggctg
    16081 gggatgggta aggtgaaatc tatcaatacg taccggctta cgccgggctt cggcggtttt
    16141 actccggtat catatgaaac aatggagtgc cgc
//
LOCUS       DAFNBJ010000107        16049 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000107 DAFNBJ010000000
VERSION     DAFNBJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.107"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            402..1541
                     /gene="wza"
                     /locus_tag="L2X49_003305"
     CDS             402..1541
                     /gene="wza"
                     /locus_tag="L2X49_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBN4798339.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLENEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1547..1990
                     /gene="wzb"
                     /locus_tag="L2X49_003306"
     CDS             1547..1990
                     /gene="wzb"
                     /locus_tag="L2X49_003306"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBN4798340.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1993..4155
                     /gene="wzc"
                     /locus_tag="L2X49_003307"
     CDS             1993..4155
                     /gene="wzc"
                     /locus_tag="L2X49_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBN4798341.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFAVC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4333..5172
                     /gene="wcaA"
                     /locus_tag="L2X49_003308"
     CDS             4333..5172
                     /gene="wcaA"
                     /locus_tag="L2X49_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBN4798342.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSREQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5175..5663
                     /gene="wcaB"
                     /locus_tag="L2X49_003309"
     CDS             5175..5663
                     /gene="wcaB"
                     /locus_tag="L2X49_003309"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBN4798343.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNIWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5660..6877
                     /gene="wcaC"
                     /locus_tag="L2X49_003310"
     CDS             5660..6877
                     /gene="wcaC"
                     /locus_tag="L2X49_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBN4798344.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6852..8069
                     /gene="wcaD"
                     /locus_tag="L2X49_003311"
     CDS             6852..8069
                     /gene="wcaD"
                     /locus_tag="L2X49_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBN4798345.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGVGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8080..8826
                     /gene="wcaE"
                     /locus_tag="L2X49_003312"
     CDS             8080..8826
                     /gene="wcaE"
                     /locus_tag="L2X49_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBN4798346.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNSIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8842..9390
                     /gene="wcaF"
                     /locus_tag="L2X49_003313"
     CDS             8842..9390
                     /gene="wcaF"
                     /locus_tag="L2X49_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBN4798347.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATLFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9417..10538
                     /gene="gmd"
                     /locus_tag="L2X49_003314"
     CDS             9417..10538
                     /gene="gmd"
                     /locus_tag="L2X49_003314"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBN4798348.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVAEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10541..11506
                     /gene="fcl"
                     /locus_tag="L2X49_003315"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10541..11506
                     /gene="fcl"
                     /locus_tag="L2X49_003315"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBN4798349.1"
                     /translation="MSRQRIFIAGHRGMVGSAITRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMVESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11509..11988
                     /gene="gmm"
                     /locus_tag="L2X49_003316"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11509..11988
                     /gene="gmm"
                     /locus_tag="L2X49_003316"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBN4798350.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11985..13208
                     /gene="wcaI"
                     /locus_tag="L2X49_003317"
     CDS             11985..13208
                     /gene="wcaI"
                     /locus_tag="L2X49_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBN4798351.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAHQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAYTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13211..14647
                     /gene="cpsB"
                     /locus_tag="L2X49_003318"
     CDS             13211..14647
                     /gene="cpsB"
                     /locus_tag="L2X49_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBN4798352.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLKTAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGAYLEEDDIVRFQDRYGRV"
     gene            14758..>16049
                     /gene="cpsG"
                     /locus_tag="L2X49_003319"
                     /gene_synonym="manB"
     CDS             14758..>16049
                     /gene="cpsG"
                     /locus_tag="L2X49_003319"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBN4798353.1"
                     /translation="MEKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEYLKPKTIVLG
                     GDVRLTSESLKLALAKGLQDAGVDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVRKGARPISGDTGLRDVQRLAEANDFPPVNESARGSYKQIDLRKEYIDHLL
                     GYINVANLKPLKLVINSGNGAAGPVVDALEARFKALNVPVTFVKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIEHCADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVSAAGGTPVMSKTGHAFIKERMREEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVTELLCLKGQTLGELVRDRMAAFPASGEINSKLAQPAEAIAR
                     VEQHFAIHALEIDRTDGISMAFPQWRFNLRSSNTEPVVR"
BASE COUNT         3910 a         4060 c         4406 g         3673 t
ORIGIN      
        1 aatagccaat taccgaattg ttatcttgcc tgctattccg ttagctgtaa cacttcctcc
       61 tgcattattg gaaatccaat attcaattac cataatgtca ccttatattc ggaagttaat
      121 ttccgtaata accctttctc atcaacgact gcatgctgag agggtgtgaa aaattaaact
      181 aaatcagtgt attggtagct aaaaagccag gggcggtagc gtgtctggat gcctgaaaga
      241 ccagtctgaa ttattctgtc aatagcctac ggatgaaatc aattttttta ggactgatgc
      301 cagttaaatt tttattcaat taactgcaag gtaaatgtgc atggcgacat tgctattaat
      361 agtgcacagg ataattactc tgccaaagtg ataaataaac aatgatgaaa tccaaaatga
      421 aattgatgcc attattggtg tcagtaacct tgataagcgg ttgcacagta cttccgggca
      481 gcaatatgtc gacgatgggc aaagacgtca tcaaacagca ggacgctgat ttcgatctcg
      541 acaaaatggt gaatgtttat ccgctgaccc cgcgcctgat tgaccaatta cgcccacgcc
      601 cgaatgtagc gcgccccaat atgacgctgg aaaacgagat cgcgaattat cagtatcgcg
      661 tcgggccggg ggacgttctt aatgtcaccg tctgggatca cccggaactc accacgccag
      721 ccggtcagta ccgcagctcc agcgacaccg gcaactgggt acagcctgac ggcactatgt
      781 tttacccgta tatcggcaag gtccacgtag tcgggaaaac gctcgctgaa atccgcagtg
      841 atattaccgg gcgcttagcg acgtacatcg ctgacccgca ggtggacgtt aatatcgccg
      901 ccttccgctc acaaaaggcc tatatctccg gtcaggtgaa taaatccggt caacaggcga
      961 tcaccaacgt gccactgact attctcgacg ccatcaacgc cgcaggtggc ctgaccgaca
     1021 ccgctgactg gcgcaacgtg gtgttaacac acaatggtcg tgaagaacgc atttctttgc
     1081 aggcgctgat gcaaaacggc gacctcaacc agaatcgact gctttacccc ggcgatattc
     1141 tctacgtgcc gcgtaatgat gatctgaaag tgtttgtgat gggtgaagtg aagaaacaga
     1201 gcaccctgaa aatggacttt agcggcatga ccctgaccga agccctgggc aatgctgaag
     1261 gcatcgacat gaccacctcc aacgccagcg gcatctttgt cattcgtccg ctgaaaggcg
     1321 agggcgggcg taacggcaag attgccaata tctaccagct ggatatgtcc gatgccacgt
     1381 cgctggtgat ggcgacagaa ttccgcctgc aaccttatga cgtggtgtat gtcaccaccg
     1441 ccccggtttc ccgctggaac cgtctgatca atcagttgct gccaactatt agcggtgtcc
     1501 gttacatgac ggatacagcc agcgacattc ataactggta atcgtcatgt ttaacaacat
     1561 cttagttgtc tgtgtcggca atatttgccg ctccccgacg gcggaacgct tactgcaacg
     1621 ttatcacccg gagctgaaag tggagtccgc tggactcggc gcgctggtcg gtaagggcgc
     1681 tgaccctact gctatcagcg tcgccgcaga acatcaactg tctctggaag gccactgtgc
     1741 acgacaaatc agccgccgtc tgtgtcgcaa ctacgacctg attttgacca tggaaaagcg
     1801 ccatatcgaa cgcttatgcg agatggcacc cgagatgcgc ggcaaagtga tgctgtttgg
     1861 tcactgggat aacgaatgtg aaatccccga tccgtatcgc aaaagccggg aaacgtttgc
     1921 agcggtgtac acattacttg aacggtctgc ccgccagtgg gcgcaggcat tgaacgcaga
     1981 gcaggtataa gaatgacaga aaaagtaaaa caacatgccg ctccggtaac gggcagtgat
     2041 gaaatcgata ttggtcgcct ggtcggcacc gtcattgaag cgcgctggtg ggtgattggc
     2101 atcaccgccg tatttgccgt ctgtgccgtg gtttacacct tcttcgccac gccgatttat
     2161 agtgccgacg cactggtaca aatcgagcaa aacagcggca attcgttagt gcaggacatt
     2221 ggttcggcat tagccaacaa accgcctgca tcggacgccg agatccagtt gattcgttcg
     2281 cgtctggtgc ttggtaaaac ggtggacgat ctcgacctcg atattgcggt gagcaaaaac
     2341 acgttcccga ttttcggtgc gggctgggat cgtttgatgg gacgtcagaa cgagacggtg
     2401 aaagtgacca cctttaaccg cccgaaagag atggcggatc aggtgtttac gcttaatgtg
     2461 ctggacgata aaaactacac cctgagcagt gatggcggct ttagcgcccg tggacaagcg
     2521 ggccagatgc tgaaaaaaga aggcgtcacg ctgatggttg aagccattca cgcccgcccg
     2581 ggcagtgagt ttaccgtcac caaatactcc acgctgggga tgatcaatca actgcaaaac
     2641 agcctgacgg taacggagaa cggcaaagac gcaggcgtac tgagcctgac ttataccggt
     2701 gaagatcgcg aacagatccg cgacattctt aacagcatcg cccgtaacta tcaggaacaa
     2761 aatattgagc gcaaatcggc ggaagcgtcg aaaagcctcg ctttcctcgc gcaacagtta
     2821 ccggaagtac gtagccgcct tgatgttgcc gaaaacaaac tgaatgcctt ccgtcaggat
     2881 aaagattctg ttgatctgcc gctggaagcg aaagcggtgc tcgattcgat ggtgaacatc
     2941 gacgcccagt tgaacgaact gacctttaaa gaggcggaaa tctccaagct gtacaccaaa
     3001 gttcacccgg cgtaccgcac gctgctggag aaacgtcagg cgctggaaga cgaaaaagcc
     3061 aaacttaacg gtcgcgtaac ggcgatgccg aaaacccagc aggagattgt ccgtctgacc
     3121 cgcgatgtcg agtctggtca gcaggtctat atgcaactgc tgaataaaga gcaggagctg
     3181 aaaatcaccg aagccagcac cgtcggcgat gtgcgcattg ttgacccggc aatcactcag
     3241 cctggtgtgc taaaaccgaa gaaagggctg attatccttg gggcgattat tcttggcctg
     3301 atgctctcta tcgtgggtgt gctgctgcgc tcgttgttta atcgcggcat cgaaagcccg
     3361 caggtgctgg aagaacacgg tatcagcgtc tatgccagca tcccgttgtc ggaatggcag
     3421 aaagcgcgcg atagcgtcaa aaccatcaaa gggattaaac gctataaaca gagccagcta
     3481 ctggcggtgg ggaatccaac cgatctggcg attgaagcca tccgcagtct gcgtaccagt
     3541 ctgcacttcg cgatgatgca ggcgcagaac aatgtgttga tgatgaccgg ggttagcccg
     3601 tcaatcggta aaacctttgt ctgcgccaac ctggcggcgg tgatcagcca gaccaataaa
     3661 cgcgtgttgt tgatcgactg cgatatgcgc aaaggctaca cccacgagct gttgggcacc
     3721 aataacgtca atggcctgtc ggaaattctg attggtcagg gcgatattac taccgcagct
     3781 aaaccgacct ctattgccaa atttgacctg atcccgcgcg gtcaggtgcc gccaaatcct
     3841 tctgaactgt tgatgagcga acgctttgcc gaactggtga actgggcgag taaaaactat
     3901 gacctggtgt tgattgatac gccgccgatt ctggcagtga ccgatgcggc aattgttggt
     3961 cgtcatgtcg gaaccacatt aatggtggcg cgttatgcgg tcaacacatt gaaagaagtg
     4021 gaaaccagtc tgagccgctt tgagcaaaac ggtattccgg tgaaaggggt gattctgaac
     4081 tccatcttcc gccgcgccag cgcgtatcag gattatggct attacgaata cgaatataag
     4141 tcggatgcga aataacgaca tccgctgtcg gatgcgatgc tgacgcatct tatccgacct
     4201 accttcacgc cgcatccggc aagcaaacca gctccgtagg ccggataagg cgctcacgcc
     4261 gcatccggca acagaacccg taaacaagct tataaaccgg cagaacatct cgatactccg
     4321 ggagaacaac ctatgaaaga caatccgctg atttcaatct atatgccgac ctggaaccgc
     4381 caacaactgg cgattcgggc gataaaatcg gtcctgcgcc aggactacag caactgggag
     4441 atgatcatcg tggatgattg ctccacctcc agggaacaac tgcaacagta cgtcactgcc
     4501 ctcaacgatc cgcgtatcac ctacattcac aacgacatta acagcggggc gtgcgcggta
     4561 cgtaaccagg cgattatgct ggcgcaaggg gaatacatta ccgggatcga tgacgatgat
     4621 gaatggacgc ccaaccgcct gagcgtcttc ctcgcccata aacagcaact ggtcactcac
     4681 gcctttttgt acgctaacga ctacgtttgc cagggcgaag tctattccca accagcaagt
     4741 ttgccgctgt atcccaaatc gccgtattca cgccgcctgt tctataaacg caatatcatt
     4801 ggcaatcagg tttttacctg ggcgtggcgt ttcaaagagt gcttgttcga taccgaactg
     4861 aaagcggcac aggattacga catcttcctg cggatggtgg tggagtatgg cgaaccgtgg
     4921 aaagtggaag aggcgacgca gatcctgcat atcaatcacg gtgaaatgca gatcacctcg
     4981 tcaccgaaaa aattctccgg atacttccac ttttaccgca agcacaaaga taaatttgac
     5041 cgcgccagca aaaaatatca gctgtttacc ctctaccaga tccgcaataa acgtatgacc
     5101 tggcgcactt tgctgacgct gctttcggtg cgtaacggca aacgtctggc tgatggtatt
     5161 cgggggcgct aaagatgctg gaagatctgc gcgccaacag ctggagttta cgtccgtgct
     5221 gcatggttct agcctatcgc gtcgctcatt tttgttcggt gtggcgcaaa aagaacgtcc
     5281 tcaacaatat ttgggcggcc ccgctgctgg tgctgtatcg cattatcacc gaatgctttt
     5341 tcggttatga aatccaggct gccgcgacca ttggccgccg ctttactatc catcacggtt
     5401 acgccgtagt gatcaataaa aacgtggtgg cgggggatga tttcaccatt cgccacggcg
     5461 tcactatcgg caatcgtggt gccgataaca tggcatgtcc acatattggc aacggcgtcg
     5521 aactcggtgc caacgttatt attcttggtg atatcacgct tggtaacaac gtcaccgtgg
     5581 gtgcgggcag cgtggtgctc gactctgtcc cggacaacgc gctggtggtg ggagaaaaag
     5641 cgcgagtgaa ggtaatcaaa tgaatattct gcaatttaat gtgcgactgg cggaaggcgg
     5701 ggcagcaggt gtggcgttag atctccacca gcgtgcgctg caacaggggc tggcgtcaca
     5761 ttttgtctac ggctacggca aaggcggcaa agagagcgtc agccatcaga actatccgca
     5821 ggtcatcaaa catacgccgc ggatgactgc aatggcgaat attgtcctgt ttcgtctgtt
     5881 taatcgcgat ctgtttggca atttcaatga gttatatcgc accattactc gcacaccggg
     5941 tccggtggtc ctgcattttc atgtgctgca cagctactgg ctaaatctta agagcgtggt
     6001 gcgcttttgc gaaaaagtga aaaaccataa accggacgtc actctggtct ggacgctgca
     6061 cgaccactgg agtgttaccg gacgctgcgc ctttaccgac ggttgcgaag gctggaaaac
     6121 aggctgccag aaatgcccga ccttaaataa ttatccgccg gtgaagattg atcgggcgca
     6181 ccagctggtg gcgggcaaac gccagttatt ccgtgagatg ctggcgctgg gctgtcagtt
     6241 tatttccccc agccagcatg tggctgacgc tttcaatagc ctgtacggtc cagggcgttg
     6301 ccggattatc aataacggta ttgatatggc aaccgaagcg attctggcgg atctgcctcc
     6361 ggtgcgcgaa acccagggta agccgaaaat cgcggtggtg gcgcatgacc tgcgttacga
     6421 cggcaagact aaccagcaac tggtgcgtga gatgatggcg ctgggcgaca aaatcgaact
     6481 gcataccttt ggtaagttct cgccgttcac cgctggtaac gtggttaatc acggctttga
     6541 aaccgacaaa cgtaagctga tgagcgcgct caatcagatg gatgcgctgg tattcagttc
     6601 tcgcgtcgat aactacccgc tgattttgtg tgaggcgcta tcgattggcg tgccggtgat
     6661 tgccacccat agcgacgcgg cgcgggaagt gctgcaaaaa tccggcggta aaaccgtcag
     6721 cgaagaagac gtgctgcaac tggtgcagtt aagcaaaccg gaaatcgcgc aggcgatatt
     6781 tggtaccacg ttggccgggt tcagccaacg tagccgcgcc gcctacagtg gacaacagat
     6841 gctggaggag tatgtcaact tctatcagaa tctgtagcta cctgctgctg ccgctgatct
     6901 acctgctggt taacgtcaaa atcgcccagc ttggtgaaag tttccccatc actatcgtca
     6961 cttttttacc tgtcttgtta ctgctgtttt tagaacgcat cagcgttaaa aaattgatga
     7021 ttgccttagg ggttggcgcg gggctcacgg cgttcaacta tctgtttggt cagtcgttgg
     7081 atgccagtaa atacgtcact tcaaccatgc tgtttgtcta tattgtgatc attattggca
     7141 tggtgtggag tattcgcttt aaaacgattt cgccacacaa ccatcgcaag atattacgtt
     7201 tcttttatct ggtggtcggg ctggtggtgg cgctggcggc ggtggagatg gcgcaaatta
     7261 tcctcaccgg tggcagcagt attatggagt cgatttcgaa atatctgatt tatagcaaca
     7321 gctatgtact gaatttcatt aaattcggcg gcaagcgcac gacagcactt tatttcgaac
     7381 cggcattttt cgctctggca ttaatctcaa tttggctcag catcaaacag tttggtatca
     7441 aaacgcctaa aacagatgct atgattctcg cagggataat attatccgga tcgttttcag
     7501 gggttatgac ctttatcctg ttttatttgc tggagtgggc attccaatat ctgaataaag
     7561 aggcgattaa gaaaaagtta ccgttagcat tgatttctct ggctgtattc ctggttggtg
     7621 tggtaatcgc gtttccttat atttccaccc gtctgggcga tttaggtacg gaaggatcgt
     7681 catcgtatta tcgtattgtc ggtccgctgg tgatggtcgg ttattctttg acccatattg
     7741 acggtgtagt cagatttggc tcactttatg aatatgtcgc atcattcgga atatttaacg
     7801 gtgcggatgt cggaaaaacc atagacaatg gtttgtatct gctgattatt tatttttcct
     7861 ggttcgcggt gtttttatcg ctgtggtaca tggggaaagt gataaaaatg atgatcaacg
     7921 cttttggtga taaccgcaat tttcgcgtgc aattatatct ttttactccg gtatcgctgt
     7981 tttttaccgg ttcgatattt agcccggaat atgcattttt aatcgtctgt ccgtttattt
     8041 tgcgaaaagc gttaaatatt acgaggtaag aataagaaca tgttgcttag cataatcact
     8101 gtcgcgtttc gtaacctcga agggatagtc aaaacacatg cctcgctggc gcatctggcg
     8161 caggcggacg atatcagctt cgaatggatt gttgtcgatg gcggttccaa cgacggtacc
     8221 cgtgagtatc tggaaaatct caatagtatc tataacctac gctttgtcag cgagccagat
     8281 aacggtatct acgacgccat gaacaagggc attgcgatgg cacaaggcaa gttcgcgttg
     8341 tttctcaact cgggcgatat ttttcatcag gatgccgcat attttgtccg taagttaaaa
     8401 atgcaaaaag ataacgtgat gatcaccggc gatgcgctgc tggattttgg cgacgggcat
     8461 aaaattaaac gtagcgccaa accgggttgg tatatttatc acagcctgcc cgccagtcat
     8521 caggcgatat ttttcccagt atccggtttg aaaaaatggc gttatgacct ggaatataaa
     8581 gtttcttctg attacgcgct ggcagccaaa atgtataaag caggttatgc atttaaaaaa
     8641 ctcaatggcc tggtgtctga attttctatg ggtggggtat ctaccaccaa taatatggag
     8701 ttgtgtgctg acgcgaaaaa agtccaacgg caaatattac atgtgcctgg cttctgggca
     8761 gaattatcct ggcatttacg ccagcgtact acctcaaaga cgaaagcctt atataacaaa
     8821 agctgaatat aagggaaaac gatgcaggat ttaagtggat tctcggtgcc gaaagggttc
     8881 cggggtggca acgctattaa agtgcaattg tggtgggcag tacaggcaac attatttgcc
     8941 tggtcgccac aagtattgta tcgctggcgg gcatttttat tacgtttatt cggcgcaaaa
     9001 ataggaaaaa acgtagttat tcggccgtca gtaaaaatta cctatccgtg gaaattaacc
     9061 ttaggtgatt acgcgtgggt cggcgatgag gtcaatttat ataccctcgg tgaaataacc
     9121 attggcgcac attcggtgat atcgcaaaaa agttatttat gcaccgggag ccacgaccat
     9181 gcaagtcaac atttcaccat taacgccacg cctattgtga ttggcgagaa atgctggctg
     9241 gcaaccgatg tctttgttgc cccaggcgtc actatcggcg acggcaccgt cgtgggggcg
     9301 cgaagcagtg tttttaaatc gcttccggca aatgtggttt gccgggggaa tcccgcagtg
     9361 gtgatacgcg aacgcgttga aactgaataa attcaaaaaa tacagaggaa taagacatgt
     9421 caaaagtcgc tctcatcacc ggtgtaaccg gacaagacgg ttcttacctg gcagagtttc
     9481 tgctggaaaa aggttacgag gtgcatggta ttaagcgtcg tgcatcgtca ttcaacaccg
     9541 agcgcgtgga tcacatttat caggatccgc acacctgcaa cccgaaattc catctgcatt
     9601 atggcgacct gagtgatacc tccaacctga cgcgcatttt gcgtgaagtg cagccggatg
     9661 aagtgtacaa cctgggcgca atgagccacg ttgcggtttc ttttgagtca ccggaatata
     9721 ccgcagacgt cgatgcgatg ggtacgctac gcctgctcga ggcgatccgc ttcctcggtc
     9781 tggaaaagaa aacccgtttc tatcaggctt ccacctctga actgtacggt ctggtgcagg
     9841 aaattccgca gaaagaaacc acgccgttct acccgcgatc tccgtatgcg gtcgccaaac
     9901 tgtatgccta ctggatcacc gttaactacc gcgaatccta cggcatgtac gcctgtaacg
     9961 gtattctctt caaccatgaa tccccgcgcc gcggcgaaac cttcgttacc cgcaaaatca
    10021 cccgcgcaat cgccaacatc gcccaggggc tggagtcgtg cctgtacctc ggcaatatgg
    10081 attccctgcg tgactggggc cacgccaaag actacgtaaa aatgcagtgg atgatgctgc
    10141 aacaggaaca gccagaagat ttcgttatcg cgaccggcgt tcagtactcc gtacgtcagt
    10201 tcgtggaaat ggcggcagca cagctgggca tcaaactgcg ctttgaaggc acgggcgttg
    10261 aagagaaggg cattgtggtt tccgtcaccg ggcatgacgc gccgggcgtt aaaccgggcg
    10321 atgtgattat cgccgttgac ccgcgttact tccgtccggc ggaagttgaa acgctgctcg
    10381 gcgacccgac caaagcgcac gaaaaactgg gctggaaacc ggaaatcacc ctcagagaga
    10441 tggtggctga aatggtggct aatgacctcg aagcggcgaa aaaacactct ctgctgaaat
    10501 ctcacggcta cgacgtggcg atcgcgctgg agtcataagc atgagtagac aacgcatttt
    10561 tatcgctggc catcgcggaa tggtcggttc cgccatcacg cggcagctcg aacagcgcgg
    10621 tgatgtggaa ctggtattac gcacccgcga cgagctgaac ctgctggaca gccgcgcggt
    10681 gcatgatttc tttgccagcg aacgcattga ccaggtctat ctggcggcgg cgaaagtggg
    10741 cggcattgtt gccaacaaca cctatccggc ggatttcatc taccagaaca tgatgattga
    10801 gagcaacatc attcacgccg cgcatcagaa cgacgtgaac aaactgctgt ttcttggatc
    10861 gtcctgtatc tacccgaaac tggcaaaaca gccgatggta gaaagcgagt tattgcaggg
    10921 cacgctggag ccgaccaacg agccttacgc cattgccaaa atcgccggga tcaaactgtg
    10981 cgaatcatac aaccgccagt acggacgcga ttaccgctca gtcatgccga ccaacctgta
    11041 tggcccgcat gacaacttcc acccgagtaa ttcgcatgtg atccctgcat tgctgcgtcg
    11101 cttccacgag gcgacggcac agaatgcacc ggacgtggtg gtatggggca gcggtacacc
    11161 gatgcgtgaa ttcctgcacg tcgatgatat ggcggcggcg agcattcatg tcatggagct
    11221 ggcgcacgaa gtctggctgg agaacaccca gccgatgctg tcgcacatta acgtcggcac
    11281 gggcgttgac tgcactatcc gcgagctggc gcaaaccatc gccaaagtgg tgggttacaa
    11341 aggtcgggtg gtttttgatg ccagcaaacc ggatggtacg ccgcgcaaac tgcttgatgt
    11401 gacgcgcctg catcagcttg gctggtatca cgaaatctca ctggaagcgg ggcttgccag
    11461 cacttaccag tggttccttg agaatcaaga ccgctttcgg gggtaatgat gtttttacgt
    11521 caggaagact ttgccacggt agtgcgctcc accccgcttg tctctctcga ctttattgtc
    11581 gagaacagtc gcggcgaatt tctgcttggc aaaagaacca accgcccggc gcagggttac
    11641 tggtttgtgc cgggagggcg cgtgcagaaa gacgaaacgc tggaagccgc atttgagcgg
    11701 ctgacgatgg cggaactggg gctgcgtctg ccgataacag caggccagtt ttacggtgtc
    11761 tggcagcact tttatgacga taacttctcc ggcacggatt tcaccactca ctatgtggtg
    11821 ctcggttttc gcttcagagt agcggaagaa gagctgttac tgccggatga gcagcatgac
    11881 gattaccgct ggctgacgcc ggacgcgctg ctcgccagta acgatgttca cgctaacagc
    11941 cgcgcctatt ttctcgctga gaagcgtgcc ggagtacccg gattatgaaa atactggtct
    12001 acggcattaa ctactcgccg gagttaaccg gcatcggcaa atacaccggc gagatggtgg
    12061 aatggctggc ggcacaaggt catgaggtgc gggtcattac cgcaccgcct tactacccgc
    12121 aatggcaggt gggtgagaac tattccgcct ggcgctacaa acgagaagag ggggccgcca
    12181 cggtgtggcg ctgcccgctg tacgtgccaa aacagccgag caccctgaaa cgcttgttgc
    12241 atctcggcag ttttgccgtc agcagtttct ttccactgat ggcgcaacgt cgctggaagc
    12301 cggatcgcat tatcggcgta gtgccaacgc tgttttgcac gccgggaatg cgcctgctgg
    12361 cgaagctctc tggtgcgcgt accgtgctgc atattcagga ttacgaagtg gacgccatgc
    12421 tggggctggg ccttgccgga aaaggcaaag gcggcaaagt ggcacagctg gcaacggcgt
    12481 tcgaacgtag cggactgcat aacgtcgata acgtctccac gatttcgcgt tcgatgatga
    12541 ataaagccat cgaaaaaggc gtggcggcgg aaaacgtcat cttcttcccc aactggtcgg
    12601 aaattgcccg ttttcagcat gttgcagatg ccgatgttga tgcccttcgt aaccagcttg
    12661 acctgccgga taacaaaaaa atcattcttt actccggcaa tattggtgaa aagcaggggc
    12721 tggaaaacgt tattgaagct gccgatcgtc tgcgcgatga accgctgatt tttgccattg
    12781 tcgggcaggg cggcggcaaa gcgcggctgg aaaaaatggc gcatcagcgt ggactgcgca
    12841 acatgcaatt tttcccgctg caatcgtatg acgctttacc cgcactgctg aagatgggcg
    12901 attgccatct ggtggtgcaa aaacgcggcg cggcagatgc cgtattgccg tcgaaactga
    12961 ccaatattct ggcagtaggc ggtaacgcgg tgattactgc tgaagcctac acagaactgg
    13021 ggcagctttg cgaaaccttt ccgggcattg cggtttgcgt tgaaccggaa tcggtcgagg
    13081 cgctggtggc ggggattcgt caggcgctcc tgctgcccaa acacaacacg gtggcacgtg
    13141 aatatgccga acgcacgctc gataaagaga acgtgttacg tcaatttata aatgatattc
    13201 ggggataatt atggcgcagt cgaaactcta tccagttgtg atggcaggtg gctccggtag
    13261 ccgcttatgg ccgctttccc gcgtacttta tcccaagcag tttttatgcc tgaaaggcga
    13321 tctcaccatg ctgcaaacca ccatctgccg cctgaacggc gtggagtgcg aaagcccggt
    13381 ggtgatttgc aatgagcagc accgctttat tgtcgcggaa cagctgcgtc aactgaacaa
    13441 acttaccgag aacattattc tcgaaccggc agggcgaaac acggcacctg ccattgcgct
    13501 ggcggcgctg gcggcaaaac gtcatagccc ggagagcgac ccgttaatgc tggtattggc
    13561 ggcggatcat gtgattgccg atgaagacgc gttccgtgcc gccgtgcgta atgccatgcc
    13621 gtatgccgaa gcgggcaagc tggtgacctt cggcattgtg ccggatctac cagaaaccgg
    13681 ttatggctat attcgtcgcg gtgaagtgtc tgcgggtgag caggatacgg tggcctttga
    13741 agtggcgcag tttgtcgaaa aaccgaatct gaaaaccgcc caggcctatg tggcaagcgg
    13801 cgaatattac tggaacagcg gcatgttcct gttccgcgcc ggacgctatc tcgaagaact
    13861 gaaaaaatat cgtccggata ttctcgatgc ctgtgaaaaa gcgatgagcg ccgtcgatcc
    13921 ggatctcgat tttattcgtg tggatgaaga agcgtttctc gcctgcccgg aagagtcggt
    13981 ggattacgcg gtcatggaac gtacggcaga tgccgttgtg gtgccgatgg atgcgggctg
    14041 gagcgatgtc ggctcctggt cctcattatg ggaaattagc gcccacaccg ccgagggcaa
    14101 cgtttgccac ggcgatgtga ttaatcacaa aactgaaaac agctatgtgt acgccgaatc
    14161 tggcctcgta accaccgtcg gggtgaaaga tctggtagtg gtgcagacca aagatgcggt
    14221 gctgattgcc gaccgtaacg cggtgcagga tgtgaaaaaa gtggtcgagc agatcaaagc
    14281 cgatggtcgc catgagcatc gggtgcatcg cgaagtgtat cgcccgtggg gcaaatatga
    14341 ctctatcgac gcgggcgacc gctaccaggt gaaacgcatc accgtgaaac cgggcgaggg
    14401 cttgtcggta cagatgcacc atcaccgcgc ggaacactgg gtggttgtcg cgggaacggc
    14461 aaaagtcacc attgatggtg atatcaaact gcttggtgaa aacgagtcca tttatattcc
    14521 gctgggggcg acacactgtt tggaaaaccc agggaagata cctctcgatt taattgaagt
    14581 gcgctccggc gcgtatctgg aagaggacga tattgtccgt ttccaggacc gctacggtcg
    14641 ggtgtaaccg cttcgcatca tgcccggtgg cgctgtgctt accgggccta cgaatgaata
    14701 aaaaatattt tgcctgttaa ttgggcgggc caactgttgc ctgaaaaagg ggtcatgatg
    14761 gaaaagttaa cctgttttaa agcctacgat attcgcggca agctgggtga agagctgaat
    14821 gaagacattg cgtggcgcat cggccgcgcg tacggcgaat acctgaaacc gaaaactatc
    14881 gtactgggtg gcgacgtgcg tctgaccagc gaatccctga agctggcgct ggcgaaaggg
    14941 ctgcaggacg cgggcgtgga cgtgctggat atcggccttt ccggtaccga agagatctat
    15001 tttgccacct tccacctggg cgtggacggc gggattgaag tcacggccag ccataacccg
    15061 atggattaca acggtatgaa gctggtgcgc aagggtgcgc gccctatcag cggcgatacc
    15121 ggcctgcgcg acgtgcagcg tctggcggaa gccaacgact tcccgccggt gaacgagtct
    15181 gcacgcggca gctacaagca gatcgatctg cgtaaagagt acatcgatca cctgctgggt
    15241 tacatcaacg tggcgaacct taagccgctg aagctggtca ttaactccgg caacggcgcg
    15301 gcgggcccgg tggtggatgc cctggaagcc cgctttaagg cgctgaacgt gccggtgacc
    15361 ttcgtcaagg tacacaacac cccggatggc aacttcccga acggtatccc gaacccgttg
    15421 ctgccagagt gccgcgacga cacccgcaat gcggtgattg aacactgtgc ggatatgggc
    15481 atcgcctttg acggcgattt cgaccgctgc ttcctgttcg acgagaaagg gcagttcatc
    15541 gagggctatt acattgtcgg cctgctggcg gaagcgttcc tcgagaaaaa tccgggggcg
    15601 aagatcattc atgacccgcg cctctcctgg aacaccgttg acgtggtgtc agcggcaggc
    15661 ggcacgccgg tgatgtccaa aaccggtcac gcgtttatta aagagcgtat gcgcgaagaa
    15721 gacgccatct acggcggcga gatgagcgcc catcactact tccgcgattt tgcctactgc
    15781 gacagcggga tgatcccgtg gctgctggtg accgaactgc tgtgcctgaa aggtcagacc
    15841 ctgggcgagc tggtgcgcga ccgtatggcg gccttcccgg caagtggaga gatcaacagc
    15901 aaactggcac agccggctga agccattgcc cgcgtggaac agcactttgc aattcacgcg
    15961 ctggaaattg accgtaccga cggtatcagc atggcgttcc cgcagtggcg ctttaacctg
    16021 cgctcgtcca ataccgagcc ggtggtgcg
//
LOCUS       DAFNBJ010000108        15750 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000108 DAFNBJ010000000
VERSION     DAFNBJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.108"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            122..364
                     /locus_tag="L2X49_003320"
     CDS             122..364
                     /locus_tag="L2X49_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798354.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            478..1116
                     /locus_tag="L2X49_003321"
     CDS             478..1116
                     /locus_tag="L2X49_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBN4798355.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1113..1850
                     /locus_tag="L2X49_003322"
     CDS             1113..1850
                     /locus_tag="L2X49_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBN4798356.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1850..3946
                     /locus_tag="L2X49_003323"
     CDS             1850..3946
                     /locus_tag="L2X49_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBN4798357.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3993..4271)
                     /locus_tag="L2X49_003324"
     CDS             complement(3993..4271)
                     /locus_tag="L2X49_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798358.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4272..4415
                     /locus_tag="L2X49_003325"
     CDS             4272..4415
                     /locus_tag="L2X49_003325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798359.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     VYG"
     gene            4541..4951
                     /gene="psiE"
                     /locus_tag="L2X49_003326"
     CDS             4541..4951
                     /gene="psiE"
                     /locus_tag="L2X49_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBN4798360.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4995..6470)
                     /gene="xylE"
                     /locus_tag="L2X49_003327"
     CDS             complement(4995..6470)
                     /gene="xylE"
                     /locus_tag="L2X49_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBN4798361.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSILQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6842..7732)
                     /gene="malG"
                     /locus_tag="L2X49_003328"
     CDS             complement(6842..7732)
                     /gene="malG"
                     /locus_tag="L2X49_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBN4798362.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7747..9291)
                     /gene="malF"
                     /locus_tag="L2X49_003329"
     CDS             complement(7747..9291)
                     /gene="malF"
                     /locus_tag="L2X49_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBN4798363.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9445..10635)
                     /gene="malE"
                     /locus_tag="L2X49_003330"
     CDS             complement(9445..10635)
                     /gene="malE"
                     /locus_tag="L2X49_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBN4798364.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11000..12115
                     /gene="malK"
                     /locus_tag="L2X49_003331"
     CDS             11000..12115
                     /gene="malK"
                     /locus_tag="L2X49_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBN4798365.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12187..13527
                     /gene="lamB"
                     /locus_tag="L2X49_003332"
     CDS             12187..13527
                     /gene="lamB"
                     /locus_tag="L2X49_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBN4798366.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13770..14690
                     /gene="malM"
                     /locus_tag="L2X49_003333"
     CDS             13770..14690
                     /gene="malM"
                     /locus_tag="L2X49_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBN4798367.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14919..15750
                     /locus_tag="L2X49_003334"
                     /pseudo
     CDS             14919..15750
                     /locus_tag="L2X49_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3960 a         3966 c         4093 g         3731 t
ORIGIN      
        1 tatcctaaaa ccccatctct aattccccgc gccgtaattc gcatgcttta gttgtgtata
       61 ctcgatcccg cccgaaatgt ttttgggtaa atctccattc attcaatgaa gggaaattgt
      121 tatgaaaaaa gttctgtatg gcatttttgc catatctgcg cttgcggcga cttctgcgtg
      181 ggctgcacct gtacaggtgg gcgaagcggc agggtcggca gcaacgtcgg tttcggcggg
      241 gagttcctcc gcgaccagcg tcagcaccgt aagctcggcg gtgggtgtcg cgctcgcggc
      301 aaccggtggc ggtgatggtt ctaacaccgg gaccaccaca accacgacca ccagtaccca
      361 gtaataaagt atgtatcccc aaaataattt gagtcattgc atctgtggct tgaagtatga
      421 agggatttac cataaccaca ctccggtgtg gttattctgc ccctctggag aagagtcgtg
      481 aagcgacctg cactcattct tatctgcctg ctattacagg cctgttcagc cacgactaaa
      541 gagctgggca attcactgtg ggacagtctg ttcggcacgc caggcgtaca gctgacggac
      601 gatgatattc aaaatatgcc ctacgccagc cagtacatgc agcttaatgg cgggccgcaa
      661 ttgtttgtgg tgctggcctt cgctgaagac ggacaacaaa aatgggtcac tcaggatcaa
      721 gccactcttg tcacacaaca tggtcgtctg gtgaagactt tgcttggcgg cgacaacctg
      781 attgaagtga ataaccttgc cgccgacccg ctgattaaac ccgcacaaat tgttgatggt
      841 gcaacatgga cgcgcacgat gggctggacc gagtaccagc aggtacgcta cgccaccgca
      901 cgctcagtct tcaaatggga tggcaccgat accgtcaagg tcggcagcga tgaaaccccg
      961 gttcgcgtgc tggacgaaga agtctccacc gaccaggcgc gctggcataa ccgctactgg
     1021 atcgacagcg aagggcaaat tcgccagtcg gaacagtatc tcggcgcgga ttatttcccg
     1081 gtgaaaacca ctctcatcaa ggcggcaaaa caatgattaa acaaactatt gtcgcattga
     1141 ttttgagtgt gggggcgtca tcggtcttcg cggcgggaac cgtcaaggta ttcagcaatg
     1201 gcagcagtga ggccaaaacg ctgacgggcg cagagtattt aatcgatctg gtaggccagc
     1261 cgcggctggc gaatagctgg tggcctggtg cggtgattag cgaagagctg gcaacggcgg
     1321 cagcattgcg gcagcagcag gcgttgctga cacggctggc agaactggct gcagattcca
     1381 gcgccgacga tgccgccgcg attaacgcct tacgccagca aattcaggcg ttgaaagtga
     1441 cgggcaggca aaaaatcaat cttgatcctg atatcgtccg cgttgccgaa cgcggtaacc
     1501 caccgttgca gggcaactac acgctgtggg tcgggccacc accgtccacg gtcacgctgt
     1561 tcgggcttat cagccgtcct ggcaagcagc cattcactcc aggtcgcgac gtggcgagct
     1621 atctctctga ccagagcctg ctcagcggtg cggatcgcag ctacgcgtgg gtcgtttacc
     1681 cggacggacg cacacaaaaa gcgccggtgg cttactggaa caagcgtcac gtagagccga
     1741 tgcccggcag cattatttat gttggcctcg cggactccgt ctggagtgag acgcctgatg
     1801 cccttaacgc cgacattctt cagactctga cgcagcggat accccaataa tgaaaaaaag
     1861 acatctgctt agcttactgg cgctgggcat tagcacagct tgctacggcg aaacatatcc
     1921 tgcgcccatt ggtccgtcgc agtcggattt cggtggcgta ggattattac aaacgcccac
     1981 cgcgcgcatg gcgcgggaag gggagttgag cctgaactat cgagataacg atcagtaccg
     2041 ttattactca gcttcagtgc aactcttccc gtggctggaa acaacgctgc gctacaccga
     2101 cgtgcgcacc cggcagtaca gcagcgtcga agcgttctct ggcgatcaaa cgtataaaga
     2161 taaagccttc gatctcaaac tgcgtttgtg ggaagagagt tactggctgc cgcaagtggc
     2221 ggttggtgcg cgggatatcg gcggtacggg gctgtttgat gcggaatatc ttgttgccag
     2281 caaagcctgg gggccgttcg attttacgct cggcctgggc tggggatatc tgggcaccag
     2341 cggtaatgtg aaaaatccgc tctgttcagc cagtgataaa tattgctatc gcgataacag
     2401 ctacaaacag gcgggatcta tcgacggtag ccagatgttc cacggtcctg cctcactgtt
     2461 tggcggcgtg gaataccaga cgccctggca accgctgcgc ctgaaactgg agtatgaagg
     2521 caataattat cagcaggatt ttgccgggaa gctggagcaa aaaagtaagt ttaacgtcgg
     2581 tgcgatttat cgcgttaccg attgggccga cgttaacctt agctatgaac gtggcaacac
     2641 ctttatgttt ggcgttacgt tgcgcaccaa ctttaacgat ctgcgcccgt cttacaacga
     2701 taacgcccgc ccgcaatatc aaccgcagcc gcaggatgcc attttgcagc attcggtggt
     2761 ggcgaatcag ttaacgctgt tgaaatacaa tgccggactt gccgatccac agatccaggc
     2821 gaaaggcgat acgctgtatg ttaccggcga gcaggtgaaa tatcgtgatt cgcgcgaagg
     2881 gatcatccgt gctaatcgga tcgtgatgaa cgatctgccg gatgggatca aaacgatccg
     2941 cattacggaa aatcgcctta acatgccgca ggtgacgacg gaaacggatg tcgccagcct
     3001 gaaaaatcat ctcgccggag agccgttggg ccacgaaacg aagctggcgc aaaaacgcgt
     3061 cgagccagtg gttccgaagt ccaccgagca gggctggtat atcgacaaat cgcgctttga
     3121 tttccatatc gatccggtgc tgaaccagtc ggtcggtggc ccggaaaact tttacatgta
     3181 tcagctgggc gtgatgggaa cggcagattt gtggctgacg gaccatctgc tgaccaccgg
     3241 cagcctgttt gcaaatcttg ccaacaacta cgacaagttt aactacacca atccgccgca
     3301 ggactcgcac ttaccgcgcg tgcgtaccca tgtgcgcgag tatgtgcaga acgatgtcta
     3361 tgtgaataac ctgcaagcca actacttcca gcatctgggc aacggcttct acggtcaggt
     3421 ctacggcggc tatctcgaaa ccatgtttgg cggtgcgggg gctgaggtgt tgtatcgccc
     3481 gctggacagc aactgggcgt ttggtctgga tgccaactac gttaaacagc gcgactggcg
     3541 tagtgcaaaa gatatgatga aattcaccga ctacagcgtg aaaaccggac atctgaccgc
     3601 ctactggacg ccatctttcg ctcaggatgt gttagttaaa gccagcgtcg ggcagtatct
     3661 ggcaggggat aaaggcggca cgctggagat cgccaaacgc tttgatagcg gtgtggtggt
     3721 gggtggctat gccacgatca cgaatgtttc gaaagaggag tacggcgaag gggacttcac
     3781 caaaggcgtg tatgtctcgg taccgttgga tctcttctcg tctggcccga cacgcagccg
     3841 tgcggcaatt ggctggacgc cgctgacgcg tgacggtggt cagcaacttg ggcgtaagtt
     3901 ccagttgtat gacatgacca gcgaccgtag cgtaaatttc cgctaagtca tgggaaaggt
     3961 gccagttttc gcactcactg gcaccttcat tcttaatggc ctgtcgagcc atcaggaacg
     4021 cagttcaccc ccggtttgcg atctggtgtg taaaggcgaa aacattctgt cttcctgtcc
     4081 gtcgatattg gttgcaggtc tggttgcctg agcgactggg ccatcattgg gctggagaca
     4141 ttgctttgcg gtaggtcggc tttatcagca gtaccgagcg gaccagcata agcaggaaga
     4201 acagagactg ataacatcaa agcagcaaaa taaggcttca tttttaccac ctttatcagg
     4261 ttacgtttca tttgttccag aggaacattg tcgatttttc gcgcattgct ggtggctggg
     4321 aatcacctga atgggtgatt tttgaattac cggctttggt gcggtttgtc ttgccggatg
     4381 cgccgccagg cgcggcttat ccggtctacg ggtaggtata tccggctttg gtggaggcgc
     4441 gctccaaatc caggttgaac aaaacataca caaaaaatat agatctccgt cacatttttg
     4501 cgttatacag gaagctcgcc actgtgaagg aggtactgct atgacgtcac tctctcgtcc
     4561 gcgcgtggag tttatctcca ccattttgca gaccgtactc aatcttggcc tgttgtgcct
     4621 cggcctgatt ttggttgtct tcctcggcaa agaaacggtg catctggctg atgtgctatt
     4681 cgcgccagaa caaaccagca aatatgagct ggtagaagga ctggtggttt actttctcta
     4741 tttcgaattt atcgcgctga ttgtgaagta ctttcagtcc ggttttcact tcccgctacg
     4801 ttactttgtc tatattggga tcaccgccat tgtgcgcttg atcatcgtcg atcataaatc
     4861 gccactggat gtgttgatct actcggctgc gatcctgtta ctggtgatca ccctgtggct
     4921 gtgtaattcg aaacggctga agcgggagta aaaagacagc acgccgaact ggcgcggcgt
     4981 gctggacagg aagattacag cgtagcagtt tgttgtgttt tcttcgtttc cggttcccag
     5041 agggcttcca gctcctcaag ggttttacct ttggtttccg ggacaaattt ccacataaat
     5101 agtgctgcca gaacgcccat acacccgtaa atccagtagg agaaaccgtt gtggaaatgg
     5161 gccaccagcc aggagttttt gtccatcatc gggaaggtcc aggagacgaa gtagttcgcc
     5221 agccactggg ccgccaccgc gattgccagc gctttaccac gaatagcatt cgggaagatt
     5281 tccgacagca gtacccagca taccggaccc caggacatgg caaaggcggc aacatagaac
     5341 agcatcgaca gaagcgccac aatacccggt gcctgagtgt aaaacgcggt accgaggcta
     5401 aacataccga ttgccattcc gagtgcgccg ataatttgca gtggcttacg accaaattta
     5461 tccaccgtca taattgccag aacggtgaag gtgaggttga taactccgac aataatggtc
     5521 tgcaacagcg cgatatccgt gctggccccc agcgttttga acacttccgg cgcgtagtac
     5581 agcaccacat tgatgccgac aaattgctgg aggatggaga gcataacgcc gatgacaatc
     5641 acgcctacgc caaacatcag cagacgacca ccggttttgc ggccatgatc cagagagtgt
     5701 ttaatttcct gtactgcctg agttgcaagg gtgttgccca taattttgcg caggatacct
     5761 tccgcctgtt cttgcttgcc gcgtgacatc agccagcgag gactttctgg cacggtatac
     5821 agcagcatta agaacagcag tgcagggata cattccgagg caaacatata acgccagccg
     5881 tcagtattca gccagctggc gtcgccggaa cgggcaataa aatagtttac gcagtaaact
     5941 aaaagctgcc cgaaaataat cgcaaactgg ttaaaagaga ccagtttccc gcgaatatga
     6001 gcaggagcaa gttccgcaat atacattggt gaaagcattg aggctaaacc aacgccaata
     6061 ccgccaataa tgcgataaat aacaaattcc gggacataac ctgccagata aacaggcact
     6121 gtgttgtccg ggtttataga ggtaaaacca agttctggcc aggcagaacc tacaccagaa
     6181 ataaaaaaca ggacagcagc aatcttaagt gaatcacgac gaccgaagcg gctactgcaa
     6241 taaccaccga gggcaccgcc gatgatgcaa ccaatcagag cgctggccac gcaaaaccct
     6301 aacagggagt tggcagcgga ttcacttaag ttttgtggag caacaaagac ggtattgagt
     6361 gactcaacag taccggaaat aacggcggtg tcgtagccaa ataataaacc acctaatgta
     6421 gcgactaagg taatcgaaaa tatataactg gaattatact gggtattcat tcagacctgc
     6481 cttagaccat tctgatcttt tatcagtgca ttgatgttgg aattgtcagg cagttaaggc
     6541 aattatcatt ttttacaatg cgtttacgta atgtcttttc tgtgatctta attgtgataa
     6601 ttatcaaaaa ttgatgaaaa caatactatt gccgtgactc agagcacgaa agagaattat
     6661 cgtaagtggg aaaacaaata acgtaaaaat aataagttct atgatggaaa tattaaccgg
     6721 cgaacgattc agattgcaga cgaaagaaaa aaaggcgctc cgtggagcgc cgaataacag
     6781 tcacaagttg ggataacgta agttgagggt gcagcggcat aacattggca gaacaacatc
     6841 tttaaccttt cacaccacct gccgtcaggc cgttgaccag ccagcgttga gccagcaaga
     6901 agacgatggt gatcggtaat gcagacatca cggcagcggc ggcaaagtca ccccacaggt
     6961 agttttgcgg gttgaggtat tgctgcattc ccacggccag ggtgtagctg tttacgtcac
     7021 gcagtaacag cgacgcgacc ggaacttcag taatggcagc gataaacgac aggatgaaca
     7081 ccaccgccag aatcggtact gacagcggca acaggacaag gcggaaggcc tgccacggtg
     7141 tcgcaccatc cagcgccgcc gcttcttcca gcgaactgtc gatggtttcg aaatagcctt
     7201 tgatggtcca gacgtgcagc gcaatcccac ccagatacgc gaaaattacg ccgccgtgag
     7261 tattcaggcc aatgaatgga atgtactcac ccagacgatc aaacaacgca tacaacgcga
     7321 ccagtgaaag tactgccggg aacatctgga aaatcagcat tcctttcagc agcgtcgctt
     7381 tgcctggaaa gcgcatacgg gcgaaagcgt aggcgcaggt ggtggagagc gccacaatgc
     7441 caatcgcgga aatcccggcg acctttaccg agttccacag ccacagcagt accgggaatg
     7501 gcggtggcgt aatgcgacca tcagcctgtt ccacgctaaa acctaatgcc agtttccagt
     7561 gatcccagga gatttgctcc gggatcaggc taccggtcgc aaagttcccc tggcgcagtg
     7621 agatggcgac gaccatcagc agcgggaaca taatcgccgc gataaaaagt agcagtagca
     7681 ggtgagtaat aaacaaacgt gctttttgcg atttcggttg gaccattgcc atttttgtta
     7741 tctcccttaa tcaaacttca ttcgcgtggc tttcaggttc actatcgcca gcgcacccac
     7801 cagcaggaag atcagcgtgg ctattgctgc tgccagaccg aagtcctgac ccccgccgcc
     7861 ttcaaaagcg atgcggtagg tgtagttaac aagcaggtcg gtataaccgg ctggcgtggt
     7921 cgtgccaaga cgatccgggc cgccgttggt taacagttga atcagcacga agttgttaaa
     7981 gttaaaggcg aagctggcga tcatcagcgg cgtcagcggt ttaatcagca gcggcagcgt
     8041 aatcttaaag aagttctgga agggacctgc accatccatt gctgaggctt catacaaatc
     8101 gtccggaatc gctttcagca agcccatgca gaggatcatc atgtacggat aacccagcca
     8161 ggtattgacg ataatcagca tcgttcgggc ggtggtcgga tcgctgaacc aggcgggctt
     8221 cacgccaaac agcgcgctca acatcatgtt gatctcaccg aagctctggt taaacaaccc
     8281 tttgaaaatc aagattgaaa tgaatgatgg caccgcgtag ggcagaatca gcaggacgcg
     8341 atagaccgct ttgccgcgca acgcttccca ctgcaccaga cacgccagaa ccatgccgac
     8401 cgccaccgtt aaaaagacag tgatcagcga gaacaccacg gtccagacga aaatggcgag
     8461 gaacggtttc tgaatgcctt cgtcggtgaa gacgcgggta aagtttttcc agccagtagt
     8521 cacggtgtaa ccggggctta gcttttcatc gccccagttg ccgtcggcgg taatggactg
     8581 gtaaaagcca atttggttat tcggacgata tttcacgccg ctctgattat tcgtcaacgt
     8641 gccgtcaccg tcgagtgtgt agagcggctg cgtgccagaa aactggcgca gggagctcat
     8701 catcactttg ttgccatccg gcagaatggc ggtaatgtca ctcagcgcct gacgattctg
     8761 ggtaatcacg cgcagatttg cgcgttcgcc ttcgggctgg gcggtcgttt ctttcagttg
     8821 cagtttttgc tcgccgccaa atttaaaagc gtcggagagg taatttttgc cggtttcgcc
     8881 gtcgctgagg gctagttgcc actcatcgcc cgccgggtaa agaccaaaat tataggtttt
     8941 gcctgcttgc caggagcgat ctaacaacac ttcctgcgca cgttcaaaag tcagctggtt
     9001 agtgctgctg tagttggtga aggcaatggc gatggtgcag accagaggga agaggacgaa
     9061 taatcccatt ccggccattc ccgggtaaac atagcgccag gcgtaggctt tacgattggc
     9121 gaaaatatac agccccgctg aactcaatat cagcgtggta atggcaaaca ggtattcccc
     9181 ttgtgcgtac attaaaacaa caaggtaacc caccagcagg ccgagcagac ctagcactga
     9241 ccatttcagc gcgtcgcttt gccaccaatg tttcttttta atgacatcca tggggttctt
     9301 cctcattcca ggacggataa ggctttcacg ccttatccga caacaactgc ctgatgcgac
     9361 actgatgcgt cttatcaggc ctacatacgt ttcggttttg taggccggac aaggcgttca
     9421 cgccgcatcc ggcatttcac agcattactt ggtgatacga gtctgcgcgt ctttcagggc
     9481 ttcatcgaca gtctgacgac cgctggcggc gttgatcacc gcagtacgca cggcatacca
     9541 gaaagcggac atctgcggga tgttcggcat gatttcacct ttctgggcgt tttccatggt
     9601 ggctgcaata cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg ctacggcacc
     9661 cagcggtttg tctttattaa ccgcttccag accttcgtca gtcagcagat agttttcgag
     9721 gaactctttc gccagctctt tgttcggact ggcggcgtta ataccggcgc tcagcacgcc
     9781 aacgaacggt ttagatggtt gacccttgaa ggtcggcagt accgttacac cataattcac
     9841 tttgctggtg tcgatgttgg accatgccca cgggccgttg atggtcatcg ctgtttcgcc
     9901 tttattaaag gcagcttctg cgatggagta atcggtgtct gcattcatgt gtttgttttt
     9961 aatcaggtca accaggaagg tcagacccgc tttcgcgcca gcgttatcca cgcccacgtc
    10021 tttaatgtcg tatttgccat tttcatactt gaacgcataa cccccgtcag cagcaatcag
    10081 cggccaggtg aagtacggtt cttgcaggtt gaacatcagc gcgctcttac ctttcgcttt
    10141 cagttcttta tccagcgccg ggatctcttc ccaggttttt ggtgggttcg gcagcagatc
    10201 tttgttataa atcagcgata acgcttcaac agcgatcggg taagcaatca gcttgccgtt
    10261 gtaacgtacg gcatcccagg taaacggata cagcttgtcc tggaacgctt tgtccggggt
    10321 gatttcagcc aacaggccag attgagcgta gccaccaaaa cggtcgtgcg cccagaagat
    10381 aatgtcaggg ccatcgccag ttgccgcaac ctgagggaat ttctcttcca gtttatccgg
    10441 atgctcaacg gtgactttaa ttccggtatc tttctcgaat ttcttaccga cttcagcgag
    10501 accgttatag cctttatcgc cgttaatcca gattaccagt ttaccttctt cgattttggc
    10561 gagagccgag gcggaaaaca tcatcgtcgt taatgcggat aatgcgagga tgcgtgcacc
    10621 tgtttttatt ttcataatct atggtccttg ttggtgaagt gctcgtgaaa acacctaaac
    10681 ggactctagt ttctttatac ggcaacctct ttccatcctc cttgccccta cgccccaccg
    10741 ttgctttgtg tgatctctgt tacagaattg gcggtaatgt ggagatgcgc acataaaatc
    10801 gccacgattt ttgcaagcaa catcacgaaa ttccttacat gacctcggtt tagttcacag
    10861 aagccgtgtt ctcatcctcc cgcctcctcc cccataaaaa agccaggggg tggaggattt
    10921 aagccatctc ctgatgacgc atagtcagcc catcatgaat gttgctgtcg atgacaggtt
    10981 gttacaaagg gagaagggca tggcgagcgt acagctgcaa aatgtaacga aagcctgggg
    11041 cgaggtcgtg gtatcgaaag atatcaatct cgatatccat gaaggtgaat tcgtggtgtt
    11101 tgtcggaccg tctggctgcg gtaaatcgac tttactgcgc atgattgccg ggcttgagac
    11161 gatcaccagc ggcgacctgt tcatcggtga gaaacggatg aatgacactc cgccagcaga
    11221 acgcggcgtt ggtatggtgt ttcagtctta cgcgctctat ccccacctgt cagtagcaga
    11281 aaacatgtca tttggcctga aactggctgg cgcaaaaaaa gaggtgatta accaacgcgt
    11341 caaccaggtg gcggaagtgc tgcaactggc gcatttgctg gatcgcaaac cgaaagcgct
    11401 ctctggcggt cagcgtcagc gtgtggcgat tggccgtacg ctggtggccg agccaagcgt
    11461 atttttgctc gatgaaccgc tctccaacct cgatgctgca ctgcgtgtgc aaatgcgtat
    11521 cgaaatctcc cgtctgcata aacgcctggg ccgcacaatg atttacgtca cccacgatca
    11581 ggtcgaagcg atgacgctgg ccgacaaaat cgtggtgctg gacgccggtc gcgtggcgca
    11641 ggttgggaaa ccgctggagc tgtaccacta tccggcagac cgttttgtcg ccggatttat
    11701 cggttcgcca aagatgaact tcctgccggt aaaagtgacc gccaccgcaa tcgatcaagt
    11761 gcaggtggag ctgccgatgc caaatcgtca gcaagtctgg ctgccagttg aaagccgtga
    11821 tgtccaggtt ggagccaata tgtcgctggg tattcgcccg gaacatctac tgccgagtga
    11881 tatcgctgac gtcatccttg agggtgaagt tcaggtcgtc gagcaactcg gcaacgaaac
    11941 ccaaatccat atccagatcc cttccattcg tcaaaacctg gtgtaccgcc agaacgacgt
    12001 ggtgttggta gaagaaggtg ccacattcgc tatcggcctg ccgccagagc gttgccatct
    12061 gttccgtgag gatggcactg catgtcgtcg actgcataag gagccgggcg tttaagcacc
    12121 ccacaaaaca cacaaagcct gtcacaggtg atgtgaaaaa agaaaagcaa tgactcagga
    12181 gatagaatga tgattactct gcgcaaactt cctctggcgg ttgccgtcgc agcgggcgta
    12241 atgtctgctc aggcaatggc tgttgatttc cacggctatg cacgttccgg tattggctgg
    12301 acaggtagcg gcggtgaaca acagtgtttc cagactaccg gtgctcaaag taaataccgt
    12361 cttggcaacg aatgtgaaac ttatgctgaa ttaaaattgg gtcaggaagt gtggaaagag
    12421 ggcgataaga gcttctattt cgacactaac gtggcctatt ccgtcgcgca acagaatgac
    12481 tgggaagcta ccgatccggc cttccgtgaa gcaaacgtgc agggtaaaaa cctgatcgaa
    12541 tggctgccag gttccaccat ctgggcaggt aagcgcttct accaacgtca tgacgttcat
    12601 atgatcgact tctactactg ggatatttct ggtcctggtg ccggtctgga aaacatcgat
    12661 gttggcttcg gtaaactctc tctggcagca acccgctcct ctgaagctgg tggttcttcc
    12721 tcttttgcca gcaacaatat ttatgactat accaacgaaa ccgcgaacga cgttttcgat
    12781 gtgcgtttag cgcagatgga aatcaacccg ggcggcacat tagaactggg tgtcgactac
    12841 ggtcgtgcca acctgcgtga taactatcgt ctggttgatg gcgcatcgaa agacggctgg
    12901 ttattcactg ctgaacatac tcagagtgtc ctgaagggct ttaacaagtt tgttgttcag
    12961 tacgctactg actcgatgac ctcgcagggt aaaggtctgt cgcagggttc tggcgtcgcg
    13021 tttgataacg aaaaatttgc ctacaatatc aacaacaacg gtcacatgct gcgtatcctc
    13081 gaccacggtg cgatctccat gggcgacaac tgggacatga tgtacgtggg tatgtaccag
    13141 gatatcaact gggataacga caacggcacc aagtggtgga ccgttggtat tcgcccgatg
    13201 tacaagtgga cgccaatcat gagcaccgtg atggaaatcg gctacgacaa cgtcgaatcc
    13261 cagcgcaccg gcgacaagaa caatcagtac aaaattaccc tcgcacaaca atggcaggct
    13321 ggcgacagca tctggtcacg cccggctatt cgtgtcttcg caacctacgc caagtgggat
    13381 gagaaatggg gttatgacta caacggcgat agcaaggtta acccgaacta cggcaaagcc
    13441 gttcctgctg atttcaacgg cggcagcttc ggtcgtggcg acagcgacga gtggaccttc
    13501 ggtgcccaga tggaaatctg gtggtaatag caaaacctgg gccggataag gcgtttacgc
    13561 cgcattcgac aaccaacgcc tgatgcgacg cttgcgcgtc ttatcaggcc tacaacggct
    13621 gtcaaatgta ggccggataa ggcgtttacg ccgcatccgg cataaaaaca ggttgtcatt
    13681 atctgaaagg ggcgaaagcc cctctgatta tcgggtttag cgcgctattg cctggctacc
    13741 gctgagctcc agattttgag gtgaaaacaa tgaaaatgaa taaaagtctc atcgccctct
    13801 gtttatcagc agggttactg gcaagcgcgc ctggaatcag ccttgccgat gttaactacg
    13861 taccgcaaaa caccagcgac gcgccagcca ttccatctgc tgcgctgcaa caactcacct
    13921 ggacaccggt cgatcaatct aaaacccaga ccacccaact ggcgaccggc ggccaacaac
    13981 tgaacgttcc cggcatcagt ggtccggttg ctgcgtacag cgtcccggca aacattggcg
    14041 aactgaccct gacgctgacc agcgaagtga acaaacaaac cagcgttttt gcgccgaacg
    14101 tgctgattct tgatcagaac atgaccccat cagccttctt ccccagcagt tatttcacct
    14161 accaggaacc aggcgtgatg agtgcagatc ggctggaagg cgttatgcgc ctgacaccgg
    14221 cgttggggca gcaaaaactt tatgttctgg tctttaccac ggaaaaagat ctccagcaga
    14281 cgacccaact gctcgacccg gctaaagcct atgccaaggg cgtcggtaac tcgatcccgg
    14341 atatccccga tccggttgct cgtcatacca ccgatggctt actgaaactg aaagtgaaaa
    14401 cgaactccag ctccagcgtg ttggtaggac ctttatttgg ttcttccgct ccagctccgg
    14461 ttacggtagg taacacggcg gcaccagctg tggctgcacc cgctccggca ccggtgaaga
    14521 aaagcgagcc gatgctcaac gacacggaaa gttattttaa taccgcgatc aaaaacgctg
    14581 tcgcgaaagg tgatgttgat aaggcgttaa aactgcttga tgaagctgaa cgcctgggat
    14641 cgacatctgc ccgttccacc tttatcagca gtgtaaaagg caaggggtaa ttacgcccca
    14701 cagtgctgat tttgcaacaa ctggtgcgtc tcctggcgca ccttttttta tgcttccttc
    14761 ctgggatatg agcgattttt tatagtaact cacttcttct tcactaagaa tatccattat
    14821 ctcaatgcct tatcagagat tcttttcctt tcgccggtag tgtctggaca ttcaggctac
    14881 ttttccaggt tattttattt ctgttatgca gaggttttat gataagtcat atcctaaatt
    14941 ctggcggcaa taactctttg atgaaacatg atgtggtgca aggaaataat atagtagatc
    15001 ttgatttact acgtaatttc aatggggtgc caggtttaaa cagagataac tttatttata
    15061 tcagcaatat tttttcaaat ataaaacaac ggaacgaaaa aatcatgcaa taaatatgtt
    15121 tcgtgaagtc tcaatcagta atgatactat aagtgtaaaa ttctacagaa atgaaaaaaa
    15181 ttgaatgcgc ttgcaatttt ctgatggata aagatgcgca ggggtatatc gacctgtctg
    15241 atttggattt aacaagttgt cattttaaag gtgacgttat atcgaaggtg tcttttttat
    15301 catcaaatct acaacatgta acattcgaat gtaaagaaat tggggattgc aattttacta
    15361 ctgcaatagt tgataatgtc atatttagat gtcgacgttt acacaatgtg atttttatca
    15421 aagcgagtgg tgaatgtgtc gatttcagca aaaatattct tgatacagtt gacttctcgc
    15481 agagtcaact tactcagagt aattttcgcg aatatcagat tagaaattca aacttcgata
    15541 attgttatct ttacgcttcg cacttcacca gagcagagtt tctgtctgcc aaagaaatat
    15601 catttattaa atcgaatatg acagctgtta tgtttgatca tgtgcgaata tcgacaggga
    15661 attttaaaga ttgcattaca gaacaattgg aattaactat tgattattca gatatatttg
    15721 ggaatgaaga tctcgatggt catatcaata
//
LOCUS       DAFNBJ010000109        15456 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000109 DAFNBJ010000000
VERSION     DAFNBJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.109"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..443)
                     /locus_tag="L2X49_003335"
     CDS             complement(<1..443)
                     /locus_tag="L2X49_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798368.1"
                     /translation="MDKYFDRSGMAIDNAKIKCIDSVKGTGEYIYRVTCNKCNGRGER
                     NHFYKSRCIACNATGYSLVTTRTCYTLTALYRIYPEAARKISAAQAAERQRAVQSKTS
                     AFNLWCQNHQELVDAITQQDGENSFLNSLKSTLSRKFPLSDKQLTV"
     gene            1303..2079
                     /locus_tag="L2X49_003336"
     CDS             1303..2079
                     /locus_tag="L2X49_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798369.1"
                     /translation="MPKYLLPAELAEIVCGLLVQPALLNELKTPATHKAFMQDIGELV
                     AKHCGGQIDGVIMPEVRDEDSQADYLTSPDETPYIVVSPGQDMHSLTDNVWRHHARDG
                     WLDHIKGSVEESPDELPTHKECAVRRYTLQRLLLQEETSHANLPVQVVRMCEYSTDDV
                     EQTDSSAEFNVTMALGGHAMLAVKDTDSRLVMALTLLIENGVPSVHVNAGDTDPSVLH
                     IRAAHGGLVLSTDSLNHDFSISPSDQYTYGRPGLLLKAAY"
     gene            2229..3005
                     /locus_tag="L2X49_003337"
     CDS             2229..3005
                     /locus_tag="L2X49_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798370.1"
                     /translation="MAKLISTRELATIITALLVKPELLGELDSPEKHRALMEDLGRVV
                     AEHCGGNVTEIALPETDGTAAEGALQNGQPVRYLETKESSPYLIVHPDASLPSVTQNV
                     WMHADHDGWEEHFNGETDALTPEMSEQFRQQVYALLTPESHTTSSEMRLTLQDWQLGE
                     AEIPEEDCQPYQVKVLAENKNVQCEVTNETGTTCFGLILEIDRGVPTLHIDTGSDSLL
                     HIHAAHDGLVLTPDAPSHGFEDAPVDRFSYNSPSLLVPGV"
     gene            3194..3535
                     /locus_tag="L2X49_003338"
     CDS             3194..3535
                     /locus_tag="L2X49_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798371.1"
                     /translation="MRELLGMAGAEHQASVMYQTFGHLDAKLGEKHKGHFVFINGQHG
                     DLCVVHSEFSSFDEGPGYFSDRADFIWELVKNDGPCSKVGIYRFDGEYALPKRRNGRR
                     FSGSVTCLQAF"
     gene            3636..3899
                     /locus_tag="L2X49_003339"
     CDS             3636..3899
                     /locus_tag="L2X49_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798372.1"
                     /translation="MTNANANDGKTSPVLPTLEQVYALLKANYKPARFADRDGEIWGH
                     EYSWNLARSSLQDLEKYGKSYVSKHSDRMGDGFSFGPDLVIIR"
     gene            4127..4831
                     /locus_tag="L2X49_003340"
     CDS             4127..4831
                     /locus_tag="L2X49_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798373.1"
                     /translation="MTMKNTVIPTVTENEMGEVITRHSAYGLVSVSRTSTTGQRLYAS
                     DLSHKEVVTMTFSESEQIERDGVIRHRLAEGRRRSPLLQVSLSPAQWATMITSFGMSD
                     GVPCTINSLIRGDYERQPEIGYIESTRERYERQIRQAAEREMAKLHKKLEVLRLLAVK
                     GKAGKRELDEAYQSLLSVINNLPVNLAFTNQLIQESMVNIVSHGKAELEATAMGVAAR
                     LGMKEMSSLASLEEKK"
     gene            complement(4864..5283)
                     /locus_tag="L2X49_003341"
     CDS             complement(4864..5283)
                     /locus_tag="L2X49_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798374.1"
                     /translation="MSELAEKPLITRVAPLERIYYCASRAEGLKPDRHEIRMQSQFLL
                     LGVIWLPPALAMAELERLLKGYDADHSRLLENVLETAHQQFLKFCEPQKREGGGVESH
                     VECPRQDDALQCLRFMSTRYALAVDLCAAYVCSRYGR"
     gene            complement(5371..7755)
                     /locus_tag="L2X49_003342"
     CDS             complement(5371..7755)
                     /locus_tag="L2X49_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HBN4798375.1"
                     /translation="MKFCRRPLIVAVYTALLFPLYSQAQLYNYSYTDTHGTKVNAEPT
                     REYLNPGSAITLNLISGLDRYERVTVTRSSDNVQMFQSVTGLVTVADRITAADGSEYY
                     GKSMTLPVLGEGTFSLKSETLDNKSNVVATTSYSFSIDITPPVLPDPMQWLRAGFQYG
                     SLDIFGDRTATQAISLSNVSDARSGLDKAEWFAIDSSGVRRTVNAQLNALTGVVQALA
                     AVAAGSTVAPVSPAYYTVGFRVYDKAGNWTETSHRSGIDRTIPSYIQHQVRNAKTGVW
                     EDYVPGMTIFENPVKFRYKRLKSDSTNFNGTDFGWVDQITNSDNTYFYTEATVSYPET
                     YMYYELHTKAGLNVNLQYKTLKFTPGNGVAEAPRYLSGQYHFRDANTWTSSDIVRFTG
                     PDCVDLGRVNVEPRSYVQTATIVGIGSCNVSVGESSCQVSMNYCKTSGKGYVPYQIHI
                     RGTGSYANLGGLQSHFYTYWDMNAPVINSIVSSPLDKTVVMLVTDSDVTNDWTNYMWA
                     TNIFNLTLKDTSNKVSTLSPKNVTVLNYQLKQVTFDYSALPSGKYTATGAATDTYNNT
                     AELTLSNTILHDVTPPVVDISFEGKQADGALVKGLENLVITLADDMTKASLKSITLSG
                     GPTSDKVSVGWYSQGTDKYGLNYPRIFPATDDADSYQLTAVAVDEAGNTTTKVSRFRY
                     VPNNLIEFNTLKTLAVGMGLKTSDNQPLAYLRTNSIRKKDGSLITGAQTGTLTVRKDA
                     AFAVSMNGVAVVPGDSKDITIDFGQGDGVLIPIFPATSGNVGESSFMIELPQIQ"
     gene            8006..8608
                     /locus_tag="L2X49_003343"
     CDS             8006..8608
                     /locus_tag="L2X49_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HBN4798376.1"
                     /translation="MKNQAIALAMAMLGVSVPASAQIYESIFTDTNGIEVHAPSTRLL
                     LNPASPVTLTLIAGLDRYVNVKITSATGTQLLNTTTTRTSVSDRLKAGDGSEFYGKKV
                     TLPVLGEGRSVVQVSILDLNQSVVATYSYNWLVDTTPLSGNALTGNAAYGSTAGSVWK
                     MGLEATGQFDFTSTNVTDVNGIEKGMFYVYRSNGSLFSTT"
     gene            8620..8856
                     /locus_tag="L2X49_003344"
     CDS             8620..8856
                     /locus_tag="L2X49_003344"
                     /inference="COORDINATES: protein motif:HMM:NF023666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HBN4798377.1"
                     /translation="MYHTYSKNSVKGAGIPDSNLDEDFTAKVVIYDNAGNTRTLPLQL
                     FRYDNYLGGPRSAKLRQRGARGFRISGLFSWDDR"
     gene            9012..9608
                     /locus_tag="L2X49_003345"
     CDS             9012..9608
                     /locus_tag="L2X49_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798378.1"
                     /translation="MVYGLENIRIALADNLTTPRITRLQITGGPTADNVELTWSPAGT
                     NVYAPEYPRLFPSFEPSENYALSVTVSDSQSNSKTYTQKFSYLPNNLVQLHNLRTLSV
                     SSALKTSLNEPLAYLSTNVLRKSNGEIAKDVQSATLTVRKDAAFGIKFNGVQAALGES
                     VEVKIDMGLGDNLLVPIYPAENGKVGTSEFMIQIDELK"
     gene            10000..10881
                     /locus_tag="L2X49_003346"
     CDS             10000..10881
                     /locus_tag="L2X49_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBN4798379.1"
                     /translation="MSSNNNKLSLVIEESLKDSGEVIQLFPTLKQTIQPKVLLRLGVF
                     VPTLKSTDKGGRKSHHIDATEPLSRLSIVEGEGYSQIQIYGPRLDMDTDFKSWCGIVY
                     ALSNRPLDSEGQLELNFPEFAKFCGFDTKRIDKQLKERFDASLTRIARTNISFIKKIP
                     GTDDQITINMHLVESTYYDSSTGKIIIKPNSKLNTLYRVDGKTRLYLKTLQKLARKES
                     AQALYLYLAELPSTFYRIGFDRLRGRLQLSSHLGNQNATVKKALGQLKEIGFLEYSID
                     KENGDYVLNILKRNMKP"
     gene            complement(11467..11982)
                     /locus_tag="L2X49_003347"
     CDS             complement(11467..11982)
                     /locus_tag="L2X49_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798380.1"
                     /translation="MKISTGMMKSNAPLIILICLMIAFSAYVKSWSLLYTLLAASVSL
                     TVMISLPFIVSPLQHSKFIALRILRLLVITLWTIGLVGVFFSVVERLVWVNADSYPAW
                     LAKELDSPAITDGENFIKATESFEKICGKNKGYLSVVTKHNGIFMRCDDSLSFDSWWK
                     GVYRLKTPETR"
     gene            complement(12633..15089)
                     /locus_tag="L2X49_003348"
     CDS             complement(12633..15089)
                     /locus_tag="L2X49_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HBN4798381.1"
                     /translation="MRKILTGCIIATAIASVYPAAAQIFSYSYTDTNQLAKTLKPATQ
                     SYLNPASTMTLNLISGLDRYERVSVTRDSDKKVMYSAVTGKISVADRIVAADGTEYYG
                     KDLALPALGEGAFTVINDTLDLQQAVVSSSTYHFTIDTTAPKYTSIYPSQNAGYEMVL
                     SGPVWGLGRGGSGQFSIFADGVDDANGIDKIRLVIKRGNGTVVSDNTLSYDTAAKRAF
                     YPWIKDAVTQPGMPTSDLNEEFTFNFIITDKAGNTLNIPPQRFLFDDQIGEYTPFAVH
                     DSRVSTSVVPGISSGYVPFVRGLSVLENPYRMVIRIPRTNWKPYRNGGITILNNYGGA
                     KVISEDTTYVYVEVKLPQGALDINYYRPVNTYQWSGGDLTQYASWLNWDPASVKSPAW
                     GSPAIERQMSDGTWFNSVNWKYFKASDMPVNLTQIRFNVQARPYDQKITGGATCNIPA
                     GSTSCTVALTQAITNGTTGYLHSGYEVRSTTESTFFMPIWENVVWHTLGPSVTGYDYN
                     DTTNILQVYINQPGDGSYFDTVKLSRVWLSDKSRNNADINVTGTLTGRNTATGNYTYE
                     FNLKLIPEGSYNVQLNAQDSFNNTGSLDYKTVTVDNTPPSISISYETKPITSSVTVYG
                     LENIRIQLADTLTKPSLTRMTLRGGPVSDAVELSWVNLGNNLYAPNYPKIFPSLNDGE
                     TYTLTVQAKDEMNNVKESAVEFSYLPNNLVRLENLKTISVSTALKTSDDTPLAVLYAS
                     QLRKKDGSIATGKQDAILTVRKDAAFGVTVNGVSAAPGESKNVQLDLGLGDSRSFPIF
                     PSTSGVVGTSEFMLNIEELK"
BASE COUNT         3919 a         3781 c         3928 g         3828 t
ORIGIN      
        1 accgtcagtt gtttatcact taatggaaat ttgcgtgata acgtcgactt caggctgttc
       61 aaaaaactgt tctcgccgtc ttgctgcgtg atggcgtcca ccagttcttg gtggttctga
      121 caccagaggt tgaacgcgga agtctttgat tgaacagcac gttgacgctc tgctgcctga
      181 gcggctgaga tcttgcgagc agcttctggg tatatgcgat acagtgcagt cagggtgtaa
      241 caggtacggg tagtgaccag gctatagcca gtggcattac aggcaataca gcggctctta
      301 taaaagtgat tgcgttcgcc acgtccattg catttattgc aagtaacacg gtaaatatat
      361 tccccggtgc cttttactga atcaatacac ttaatcttag cgttatcgat tgccattccg
      421 ctacggtcaa agtatttatc catttttgat tcctcaggtt tggtctgaag taataatgac
      481 agattggcct cgctggctaa taacctgaac gaacgttttt tgaaaataaa tcaggaagga
      541 tgattgagat gatttatata tgcaggattc aattttaatg gagggtattt tcagaattat
      601 ctgtttacaa aaaacgaaac aaatcctaat gaggcattta ttgcatcaca ataaaagtga
      661 ttgcttttca ctcatttgag tagcgatgct taagtattta tccgttttaa ggtataacag
      721 cagcgtgata cggctcaggg aatgcctgtc cgtcacctta atgatgcttt acttccggtt
      781 gccgaaacag tccatatggc tgacatgtat aacacgcacg gtctaacgca gttccggggg
      841 gtggcacagc ggcgtgatgc cgctttaatc tggaaaacgc aggcatctac tggtgcctca
      901 ggatcgcggc actcgcaagc tgaaacacca gagcctgttc gtcttccggc tgtatcgctc
      961 gtccccgtct acaaaacccg tctcatccgc aggtatcgct ggcgcactcc cagacggtta
     1021 gaggctttcg ggaactgcct gggtcaaaga acaccggcgt tcaggtagct gttgaaatgg
     1081 acatctacac gtttttgttc aagtgattca gttgtggtga gggcataacg ggtaatgccc
     1141 tcccgtgaag ccctgagaaa agttactttt ccaggattaa tgcagtcttg cttttaatat
     1201 aaattcaggg gatatttttt taaaaaaata cctgtcgtca acttattaga aaagtaagtc
     1261 aaaatgctag agtgttctca ttcaaaaaga ggagaactaa ccatgccaaa atatttgtta
     1321 cctgccgagc ttgcggaaat tgtttgtgga ttactggtgc aaccagcatt attgaatgaa
     1381 ttaaaaacac cagccacgca taaagcattt atgcaggaca tcggcgagct ggtggcgaaa
     1441 cattgcggcg gtcaaattga cggcgtgatc atgcctgagg tacgcgatga agacagccag
     1501 gccgattacc tgacatctcc agatgaaacc ccttatattg ttgtctctcc tggccaggac
     1561 atgcactccc tcacagataa cgtctggcgt catcatgccc gggatggctg gcttgaccat
     1621 atcaaaggca gtgtcgaaga aagccctgat gaactgccga cccacaaaga gtgcgctgta
     1681 cgccgctaca ccctccagcg tctgctcctt caggaagaaa ccagccatgc taatctgcca
     1741 gtacaggtgg tacggatgtg tgagtacagc accgacgatg ttgagcagac cgattcttca
     1801 gcagagttta acgtcacaat ggcgctcggt ggtcatgcca tgctggcagt aaaagataca
     1861 gattcccgtc tggtgatggc cctgacactc ttgattgaaa atggagttcc ctctgtccac
     1921 gttaatgcgg gtgacactga tccttcagtt ctgcacatcc gggcagctca cggtggtctg
     1981 gttctttcaa ccgactccct taatcatgat ttcagtattt cgccctctga ccagtatacc
     2041 tacggacgcc ctggattatt attgaaagcc gcctactgat ttattaattc aaaatccgtc
     2101 attattattt acccccattt ttcgccccta acttatgggg cgagtatatt ttaaaaaact
     2161 ttctttcagg ttattagcca gcaaactaga actggtagaa ttaattcatc agagaaaagg
     2221 ggactatcat ggctaaatta atttcgacgc gtgaactggc aacaattatt actgcattgc
     2281 tggtaaaacc agaattactg ggcgagctgg attccccaga aaaacatcgc gccctgatgg
     2341 aagatctggg gcgtgttgtg gcagagcatt gtggcggtaa tgtcactgaa atcgcactac
     2401 cggagaccga tggtacggct gctgaaggcg cgttgcagaa tggccagccc gtccgctatc
     2461 tggaaaccaa agaaagctct ccgtatctca tcgtacaccc tgacgcctca ctgccgtcgg
     2521 tgacgcaaaa cgtctggatg cacgctgatc atgatggctg ggaagagcat ttcaacggag
     2581 agaccgatgc cctgactcca gagatgagcg agcagttccg ccagcaggtt tatgcactgc
     2641 tgacgccgga atcccatacc acctcttcag aaatgagact gactttgcag gactggcaac
     2701 tgggtgaagc cgagatccca gaagaagact gtcagcccta ccaggtgaag gtgttagcag
     2761 aaaataaaaa cgttcagtgc gaagtgacga atgaaaccgg caccacgtgt ttcggcctca
     2821 ttttggagat cgaccgtggt gtaccgacgc tgcacattga tacgggcagt gattcactgc
     2881 tgcacattca cgcggcacat gacggcctgg ttctgacacc ggatgcaccg agccacggat
     2941 ttgaagatgc tcctgtagat cgcttttctt ataacagccc gtccctgttg gtgcctgggg
     3001 tgtaaccagg caaactgccc agcggtattt tggccccggg taaaattatt cagaacagga
     3061 gtcaggcaga tgggcgatgt tgccacacta ccaccgaaaa ttaacgatgg cgtacttcga
     3121 cgctggaatg ctctcggtaa agcggataaa gaggcgctgg ccgactatgg cgttagcgtg
     3181 aaggaggagt gaattgcgcg aattacttgg aatggcaggt gcagaacatc aagcctccgt
     3241 aatgtatcaa acgttcggac acctcgacgc gaagctaggc gaaaagcata aaggtcactt
     3301 cgtttttatc aacggccagc acggcgatct gtgcgttgtg catagtgaat tttcatcctt
     3361 tgacgaaggt ccaggctatt tcagtgaccg tgccgacttc atctgggaat tagttaaaaa
     3421 cgacggccca tgctctaagg tcgggattta ccgatttgac ggcgaatacg cgcttcctaa
     3481 gcgccggaat gggagaagat tttcaggaag cgtaacgtgc ctgcaagcat tctaattccg
     3541 tgctgcatga gcgccgacag gtcttccgta caacgggagg cggcacagtt cgcgtgcaac
     3601 catccccaca caataactcg actgagtaca taataatgac gaacgctaac gcaaatgacg
     3661 ggaagaccag cccggtatta cccactctcg aacaggtcta tgctctgctc aaggccaact
     3721 ataaacccgc acgatttgct gatcgggatg gggaaatctg ggggcatgag tattcatgga
     3781 acttggcaag atccagtttg caagacctgg agaaatacgg gaaatcatat gtatccaagc
     3841 attccgaccg gatgggcgat ggattcagtt ttggtcctga cctggtcatt attcgctgac
     3901 atacgcacgg aagtgagtaa gatggctggc tgcgccggag aactagagta atgaagggta
     3961 aaaatatcgt ggctcgtgtc gtgcgggaag cggcctataa gtgggaaact cgccaccaat
     4021 tgcagcaggc gtacaattca gcgagtgacg tgggaaaagt tcccagacat tccggttccg
     4081 catctgattt acgccagacg ctgtcatttt taattctttg tagggcatga cgatgaaaaa
     4141 cacagtaata ccgacggtga ctgaaaacga gatgggagag gtgatcaccc gccactcggc
     4201 ttacggactg gtatcagtaa gccgcacgtc cacgacggga cagcgccttt atgcctccga
     4261 cctcagtcat aaagaggtag tcacgatgac cttcagtgaa tcagagcaga ttgaacgcga
     4321 cggtgtaatc cgtcatcgac tggcggaagg ccgacgccgc tcaccgctcc tgcaagtcag
     4381 tctgtctccg gcgcagtggg ccacgatgat cacatcgttc ggaatgagtg acggcgtgcc
     4441 gtgtaccatt aacagcctga ttcgcggcga ttatgagcgg cagccggaga ttggctatat
     4501 cgagtcgaca cgcgaacgat acgaacggca gatccggcaa gccgctgagc gtgaaatggc
     4561 gaaattgcat aaaaagctgg aagtgctgcg tttgctggcg gtaaaaggca aggctggaaa
     4621 gcgtgagctt gacgaagcct atcagtcgct gttaagcgta atcaataact tgccggtcaa
     4681 tctggccttc accaaccaac tcattcagga atccatggtg aatatcgtct cacacggcaa
     4741 ggccgaactc gaagccaccg ccatgggcgt agccgctcgt ctgggtatga aagaaatgag
     4801 cagccttgcc tcgctggaag agaagaagta accggtgcgc ccgcccgtaa atcatgaaat
     4861 gcactaacgt ccatacctgc tgcacacata cgcggcacac agatcaacgg ccagcgcata
     4921 ccgcgttgac ataaaccgta aacactgaag tgcgtcgtcc tgtcggggac attctacgtg
     4981 gctttctacg cccccccctt ctctcttctg cggctcacag aacttcagga attgctggtg
     5041 ggcagtctcc aggacgtttt ccagtaaccg gctgtggtca gcgtcatatc ccttcagcag
     5101 gcgttccagt tctgccatag cgagtgcagg tggtaaccag atgacgccaa gcagcaaaaa
     5161 ctggctctgc attcgaattt catggcgatc tggcttcaga ccctctgccc gggaagcgca
     5221 ataataaatc ctctccaggg gagctacacg cgtgataaga ggtttctcag caagttctga
     5281 catgcgacta aatcagcctg tttcgtttct ttctgattac gttaagacac tatgaaaaag
     5341 ccccgtaata cggggccggt tcggaggcag ttactggatt tgcggcagct cgatcatgaa
     5401 gcttgattca ccgacattgc cgctggtggc cgggaagatg gggatcagga cgccatcacc
     5461 ctgcccgaaa tctatcgtga tgtctttcga atccccgggt acgacggcca ccccattcat
     5521 actaaccgca aaggccgcat ctttgcggac cgtcagcgtg ccggtttgtg ctccagtgat
     5581 cagcgaacca tcttttttcc ggatgctatt ggtacgcaga tacgccagcg gctggttatc
     5641 tgatgtcttc agccccatac ccacggccag cgttttgaga gtgttaaatt caatcaggtt
     5701 gttcggtaca taacggaacc ggctgacctt tgtcgtggta ttaccggctt catctaccgc
     5761 aaccgcagta agctggtagg aatccgcatc atctgtcgcg gggaagatac gcgggtagtt
     5821 cagcccgtat ttatcagttc cctggctgta ccagcccaca ctgactttgt cagaagtggg
     5881 tccgccgctc agcgtgatgc ttttcaggga agcttttgtc atatcgtcgg ccagtgtgat
     5941 caccagattt tccagccctt tgaccagtgc tccgtcagcc tgctttccct caaaactgat
     6001 atcgacaaca ggcggcgtga cgtcatgcag gatggtattc gatagcgtca gttctgccgt
     6061 gttgttgtag gtgtccgtcg cggcacccgt tgcagtgtat ttcccggagg gtaacgcgct
     6121 gtaatcaaac gtcacttgtt tcagctggta gttcaggacc gtaacgtttt tcggcgacaa
     6181 ggtagagact ttattactgg tgtccttcag tgtgaggtta aagatattgg tggcccacat
     6241 ataattggtc cagtcgttag ttacgtcact gtccgtcacc agcattacca ccgttttatc
     6301 cagtgggctg gagacgatac tgttgataac cggggcgttc atatcccagt aggtgtagaa
     6361 atgcgactgt agtccgccga gatttgcgta tgaaccggtc cctctgatat gaatttggta
     6421 cggaacatag cctttcccgc tcgtcttaca gtaattcatg ctgacctggc aactgctctc
     6481 acccactgaa acattgcaac tgccaatgcc gacgatagta gcagtttgca cataagagcg
     6541 cggttcaacg tttacacgac ccaaatctac gcaatccggc ccggtaaacc tgacgatgtc
     6601 actcgaagtc catgtattag catctctgaa atggtactgc cctgacaaat agcggggggc
     6661 ttcagccacg ccgttacccg gagtaaattt cagcgttttg tactggaggt tgacgttaag
     6721 gcccgcttta gtatgaagct cgtagtacat ataggtttcc gggtagctca ccgtggcttc
     6781 agtataaaaa taggtattgt cggagttggt aatctgatcg acccagccga agtctgtccc
     6841 gttaaaattg gttgagtccg atttaaggcg tttgtacctg aatttgactg ggttctcgaa
     6901 aatagtcatg ccaggaacat aatcttccca gacgcctgtt ttcgcattcc gcacctgatg
     6961 ttgaatgtag ctgggtattg tacgatcaat accagagcga tgtgatgttt ctgtccagtt
     7021 cccggctttg tcataaaccc gaaaacctac ggtgtaatat gccggactga cgggcgcaac
     7081 cgtgcttcct gccgccaccg ccgccagtgc ctggaccacc cccgtgagtg cattcagctg
     7141 agcatttaca gtccggcgga caccgctgct gtcaatagca aaccactccg ctttatccag
     7201 tcctgacctc gcatcagaga cgttcgataa cgaaatggcc tgggttgcgg tcctgtcgcc
     7261 gaaaatatcc agacttccgt actgaaaacc agccctgagc cactgcatcg gatctggtag
     7321 aacgggagga gtgatatcga tgctgaaaga gtagctggtt gtcgccacga cattcgactt
     7381 attgtcgagt gtttcggatt taagagaaaa agtaccctcg cccagtaccg gcagtgtcat
     7441 ggacttgccg tagtactcac taccatcagc ggcagtgatc ctgtcggcca ctgtcaccag
     7501 gccggtgaca ctctggaaca tctgcacatt atcggatgaa cgtgtcacgg taacgcgctc
     7561 gtagcggtcg agaccagata tcagattcag cgtaattgcg ctgcccgggt tgagatactc
     7621 tctggtgggt tcagcattca ctttcgtgcc gtgtgtgtcg gtatacgaat agttgtaaag
     7681 ctgagcctgg ctgtaaagcg ggaacagcag tgccgtatag acggcgacaa tcaacggacg
     7741 tcggcaaaat ttcatttcgg ttctcctggt tggccagaaa attttacctg aatcgttttc
     7801 ttcgcccggg cgctaagagg ggtaattgtt cagtaaaaca tcgtccgttt gtgtgctttt
     7861 tgccagattt aatcggcagg cctgcgggaa ataccccatc ccaatgtccg ttttaggctg
     7921 attaattccc cagaaatccc ccccttaacg gaaaaatccg cagggcagac gtttaaaata
     7981 gcaacaacta aaaggggaat actgaatgaa aaatcaggct atagctttgg ctatggctat
     8041 gctcggagtc tcagttccgg catctgctca aatttatgaa tccatcttca cagataccaa
     8101 tggtattgaa gttcacgccc cctcaacccg gctgttgctg aatccggcct caccggtaac
     8161 cctgacgctc attgccggtc ttgaccgtta tgtgaatgtc aaaatcacca gcgccaccgg
     8221 cacgcaactg ctgaacacca ctactacgcg caccagcgta tccgaccgtc tgaaggccgg
     8281 agatggcagc gaattttacg gaaaaaaagt aactttaccg gtgcttggcg aaggcaggtc
     8341 ggttgtgcag gtcagtattc tggatctgaa tcagtccgtt gtagccacct acagctacaa
     8401 ctggctggta gacaccacac cgctgtctgg taatgccctc acaggcaacg ccgcctatgg
     8461 atcaactgcg ggtagcgtct ggaaaatggg gctggaggcc acggggcagt ttgattttac
     8521 ctcaaccaat gtcacggacg taaatggcat cgagaaaggt atgttttatg tctatcgcag
     8581 taacggcagt ttgttcagta cgacgtgact ggtcagaaga tgtatcacac ttactcaaaa
     8641 aactcggtca aaggcgcggg tattcccgac agcaatctgg atgaagattt cacggccaag
     8701 gtagtcattt atgacaacgc aggaaacacc cgaacccttc cgttgcagct ctttcgatat
     8761 gataactatc tgggcggtcc gagatccgca aagctccgcc agcgtggtgc caggggtttc
     8821 cggatatccg gcctattcag ctgggatgac cgttaatgag aatccgattc atcttgtata
     8881 tcgcgtgcca aagtccaact accggtcata tgccgagggc ggcctactat tctataacct
     8941 gtattcttcc ccgaccgaga ttgcgacgga cagcacctat acctacgttg aaatgatcct
     9001 gccctagtac ggtggtatac gggctggaga atatccgtat agctctggcg gataacctga
     9061 caacaccccg gatcacccgt cttcagataa ccggagggcc aacggcagat aacgttgagc
     9121 tcacgtggtc acctgctggc acgaacgttt atgcgccaga atatccaagg ctgttcccct
     9181 cattcgagcc ttccgaaaac tatgcgctta gcgtgacggt ctccgacagt cagtcgaaca
     9241 gcaaaactta tacccagaaa ttcagctacc tcccgaataa cctagtgcag ttgcataacc
     9301 tgcgaacctt atccgtcagt tcagcactca aaacgtcgtt aaatgagcct cttgcttatc
     9361 tgtcaaccaa cgtgttgcgt aagtccaacg gtgagattgc aaaagacgtc cagagtgcga
     9421 cgctaacggt acgtaaagac gctgccttcg gtattaagtt caacggcgtg caggccgcgc
     9481 ttggtgaatc tgtagaggtg aaaattgata tggggctcgg tgataacctt ttagtaccta
     9541 tataccctgc tgaaaacggc aaagtgggta catccgagtt catgatccag atcgatgagc
     9601 tgaagtaatg agaatgggcc gtcggtagta ctggcggctt aacgaaagta actttttctg
     9661 taagggttga agtgtgcttc tgatgttttt agaacggatc ggaatttact cacattttga
     9721 tcgtgatgat tcaatacgtg acagagcata gttatcaaca ggatagaata ataataaaga
     9781 tcaatagagg atcaatagca gatcaaaaag gaataacgat cctcgcagcc cagtgatatc
     9841 gggcctctca gcggatcagc atccactgta cagggggaga aatacactat gtcgggtaat
     9901 ccatccactg taccgggcgc ataatccact ataccgggta gattcgatac actatagcgg
     9961 gtaggatgta gatcttatgc acaaaacgga tctcccaata tgtcaagtaa taacaacaag
    10021 ttatctctcg tcattgaaga gtccctaaaa gattcagggg aagttataca actctttccc
    10081 acattaaaac agaccattca accgaaagtt ttactccgcc taggtgtgtt tgtgccgact
    10141 ttgaaatcta cggacaaggg ggggaggaag agccatcata tcgatgccac tgagccgcta
    10201 tcaaggttat ctattgtaga gggtgaaggt tacagccaga tccaaatcta cgggccgcgt
    10261 ctcgatatgg ataccgattt caaatcatgg tgtgggatcg tttatgcgtt gtctaaccgg
    10321 ccgcttgatt cagaaggtca gctggagctg aattttccgg agtttgcaaa attttgtggg
    10381 tttgatacca agcgcattga taaacaactg aaagagcgct ttgatgcttc gctgacgcgt
    10441 atagcccgga ccaacatttc atttattaag aaaatacccg gtacagatga tcagataacg
    10501 ataaacatgc acctggtgga atcgacgtat tacgacagtt ccacgggaaa aatcatcatc
    10561 aagccaaatt cgaagctcaa tacgctgtat cgcgtcgacg gtaaaacccg actctacctg
    10621 aagacattgc aaaaactggc ccgtaaggag tccgcacagg ccctgtacct gtatctggct
    10681 gagctgccga gcacctttta ccgcatcgga ttcgatcggc ttcgtggacg actccaactg
    10741 tcatcacacc tcggtaacca gaatgccacg gttaaaaagg cgctgggcca gcttaaagag
    10801 atcggttttc tggaatactc catagacaaa gaaaacggcg attacgtact gaatattcta
    10861 aagagaaaca tgaagccatg accgatgccg gataatgcac ctgcctgccg cgtgggtggc
    10921 tgcgttaccc ggtatagtgg acattcttct cctttccact ctgctttacc cggtacaatg
    10981 gatctccact acaccgggta gtgtcaggcc taaaattacg atgcagcccc tcctcttcag
    11041 ccttacactg aatatttagt gtgttttttc accaaaaatg tgatctatac ccggtacagt
    11101 ggagtcacag aaatgtgatc cgtacccggt atagtggatg agaactactg ctaatcggtt
    11161 accgggaatg tggtggtact gggtggaaag gtttacccgg tatagtggat cgtggcagtg
    11221 tcatctttcc ggatagcatg acgtcacggt taggaaaccg tacccggtat agtggatagc
    11281 gaatggtgta caaccctgtt aacgtgatga tgctgaagtg aaagtttaac ccggtttagt
    11341 ggagcgactt aaagcgcgta ccagtagctg atgggggagc agcacccggt acagtggagg
    11401 ggcggatatc gtcgtaaagg tcttaactaa aagatgacca ttcagtcgca gcattaaaga
    11461 aatatatcat cgagtctcag gggttttaag ccggtagaca cccttccacc aggagtcgaa
    11521 agacaagcta tcatcgcagc gcataaatat gccgttatgc ttggtgacaa cgctaagata
    11581 gcctttattt ttcccgcaga tcttctcaaa agactctgtt gctttaataa aattttcacc
    11641 atccgtgatg gccggagaat ccagttcctt cgccagccat gccggatagc tgtccgcatt
    11701 aacccataca aggcgttcaa caactgaaaa gaagacgccg acaaggccaa tcgtccaaag
    11761 cgtaataact agtaaacgca aaattcgtaa ggctataaat tttgagtgct gtagaggcga
    11821 aacgataaaa ggcagtgaga tcatgactgt gagtgagact gatgccgcca gcaaggtgta
    11881 taacaatgac catgatttaa cataggcgga aaaagcgatc attaaacaaa tcagaatgat
    11941 gagcggtgca ttgcttttca tcatgcccgt gctaattttc attcttttgg ggctccattt
    12001 tgtacgagtt ttgagagact gcgtttgtaa tcatccgctg ccttagaaaa gttaccgtca
    12061 ttctgtaccg gcgtaaagcg aatgccaaaa aacgcctcaa tgacggagga aagcgtcact
    12121 atcggtcctg acgaggcatg ggcttttcgc tcccgagcct cacgctccgc aaagtggatc
    12181 accggtcccc tagaaaatga gagtgagtca tgggaagacc agtctgttta tagccacagt
    12241 agttaaacgc accccgccag tccgtcgctg tttgacatca tggccgcgaa ttaggttaac
    12301 gtcgcccggt atagtgtaat cccaggctga acctgtgttt gcagctggtg gccctgcatc
    12361 catcaccatg acaggcgctg gccgcaggaa tcgcgtgccg gatgaagggt atccactata
    12421 ccgggtgaat gatatgttga tccacgctga cgggtagttt tatgagcaaa cccgcagcga
    12481 gtggcaggcc tgtgtcattc gcagttttac tgatgggacg gaaggttatc cacaaaaacg
    12541 gtgaataacc ctgtgaccgg aatctggata atcatcattg cggcgacaac aacagaaaga
    12601 agtggcccgg acttggccac cctaccctgc ttttacttca gttcctctat attcagcata
    12661 aattcagaag ttccaacgac gccggaagtc gaaggaaaaa tagggaagct gcggctgtca
    12721 cccagaccca gatccaactg aacatttttg ctctccccag gagctgcgga aaccccattc
    12781 accgtgacgc cgaaggctgc atccttacgc accgtcagta tcgcgtcctg tttcccggtt
    12841 gcgatagacc cgtccttctt acgcagctgg ctggcataaa gaaccgccag cggtgtgtca
    12901 tcggacgttt tcagcgcggt actaacggag atggtcttaa ggttttccag acgaaccagg
    12961 ttgttcggca agtaactaaa ttcgacggcg ctttctttaa cgttgttcat ctcgtctttg
    13021 gcctgcacgg taagcgtgta agtttcgccg tcgttaagtg aaggaaagat cttcgggtaa
    13081 ttcggggcat aaagattatt tccgaggttt acccacgaca actcaacagc atcagatacc
    13141 gggccaccgc gaagcgtcat tcgcgttaaa gagggcttgg tcagggtatc tgcgagctga
    13201 atacgaatgt tctccagtcc atacaccgtt acactgctgg tgataggttt ggtttcgtag
    13261 ctgatactga tggacggcgg cgtgttatcg accgtcacag tcttatagtc cagactaccc
    13321 gtattgttga acgaatcctg ggcgttgagc tgcacgttat agctgccctc cgggattagt
    13381 ttcaggttga actcgtatgt ataattcccg gttgctgtgt tacgccccgt tagtgttccg
    13441 gtcacattga tatccgcgtt attgcgggat ttgtcggaca gccacacccg gctcagttta
    13501 acggtatcga aatacgatcc gtctcccggc tggttgatgt aaacctgaag gatgttggtc
    13561 gtatcattgt agtcatagcc agtgaccgaa ggccccagcg tatgccagac gacgttttcc
    13621 cagataggca tgaagaaggt ggattctgtt gtggaacgta cctcataacc actatgcaga
    13681 tagccggtgg tgccgtttgt aatcgcctgt gtcagggcga cggtacacga cgttgacccg
    13741 gcagggatgt tgcaggtggc accgccagta attttttggt cgtagggcct tgcctgcaca
    13801 ttgaaacgga tctgggtcag gttcaccggc atatctgaag ccttgaagta tttccaattg
    13861 acgctgttaa accaggtacc atcggacatc tgtcgctcaa tcgccggact cccccatgca
    13921 ggacttttta ccgaagccgg gtcccagttc agccaggagg cgtattgcgt cagatcgcct
    13981 ccggaccatt ggtacgtgtt cactgggcga tagtagttta tatccagagc gccctgggga
    14041 agcttcacct cgacgtacac ataggtcgtg tcttctgaaa taaccttagc accgccataa
    14101 ttgttaagaa tggtgatgcc gccgttacgg tatggcttcc agttggttcg ggggatacgg
    14161 atgaccatac ggtatgggtt ttcgagtaca gatagccccc gaacaaacgg gacgtaccct
    14221 gaagagatcc ccgggaccac actcgtactg acgcgtgagt catgaaccgc aaagggggtg
    14281 tattcaccta tctggtcatc aaataaaaag cgctgcggcg gaatattcag cgtgtttccg
    14341 gctttatctg tgatgataaa attgaaggta aattcttcat tgagatcgct ggtgggcata
    14401 ccgggttgag tgaccgcgtc cttgatccag gggtaaaagg cacgttttgc cgccgtatcg
    14461 taactgagtg tgttgtcgga cacgactgtg ccatttccgc gcttgatgac cagacggatc
    14521 ttgtctatgc cattcgcatc atcaactcca tcagcaaaga tggaaaactg gccagatcct
    14581 ccgcgcccaa gcccccaaac tgggccactc aggaccattt catagcctgc gttctggctc
    14641 gggtagatgc tggtatactt tggcgctgta gtgtcgattg tgaaatgata tgttgaagag
    14701 ctgacgacag cctgctggag atccagagta tcgttgatga cggtgaaggc cccttcaccg
    14761 agtgcgggta acgccaagtc ctttccgtaa tattccgtgc cgtctgcggc aacaatgcga
    14821 tctgcgacgc ttatctttcc tgtcactgcg gaatacatga cctttttgtc actgtccctg
    14881 gtcacactca ctcgttcgta tcggtcaagg ccggagatca gattcagggt cattgtgctt
    14941 gccggattaa ggtagctctg cgtggcaggc ttaagcgtct ttgccagctg attggtgtca
    15001 gtatacgaat agctgaaaat ctgagccgcc gccggataaa ctgaagctat ggccgtggca
    15061 atgatacagc ctgtcagaat tttacgcatg tgatactccc gggatgtatt tgcccctatt
    15121 ttacgaaatc ttttcacctg ggcttgctgg ttagccgggg tatttcacgg cataaaactt
    15181 ttaaatgttg ccgtgatagc aatgttgtcg ggtcagaaca aaacgaaagt taaggcaact
    15241 tcaacagggc tgattacggt ggaagaaaag taacttttct cttgagaaga aataggaaaa
    15301 gcagaaatta ggacgattaa atgaggtgaa atgtcagcaa caggcgatga ttgtccatca
    15361 tcgtaaggga gggtacaggg tgccgctgcc ttactggcat catgttgatg tccagctctt
    15421 catacccgtt tgtaccttag ccggaactgt tgttca
//
LOCUS       DAFNBJ010000110        15239 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000110 DAFNBJ010000000
VERSION     DAFNBJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.110"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            105..671
                     /locus_tag="L2X49_003349"
     CDS             105..671
                     /locus_tag="L2X49_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBN4798382.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISSADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSIMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            675..1250
                     /locus_tag="L2X49_003350"
     CDS             675..1250
                     /locus_tag="L2X49_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBN4798383.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1292..2344)
                     /locus_tag="L2X49_003351"
     CDS             complement(1292..2344)
                     /locus_tag="L2X49_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBN4798384.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2569..3489
                     /gene="lpxL"
                     /locus_tag="L2X49_003352"
     CDS             2569..3489
                     /gene="lpxL"
                     /locus_tag="L2X49_003352"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBN4798385.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3661..4887
                     /gene="mdtG"
                     /locus_tag="L2X49_003353"
     CDS             3661..4887
                     /gene="mdtG"
                     /locus_tag="L2X49_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HBN4798386.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4970..5344
                     /locus_tag="L2X49_003354"
     CDS             4970..5344
                     /locus_tag="L2X49_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBN4798387.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5345..5572)
                     /locus_tag="L2X49_003355"
     CDS             complement(5345..5572)
                     /locus_tag="L2X49_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBN4798388.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5745..8288)
                     /gene="mdoH"
                     /locus_tag="L2X49_003356"
     CDS             complement(5745..8288)
                     /gene="mdoH"
                     /locus_tag="L2X49_003356"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBN4798389.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8281..9816)
                     /gene="mdoG"
                     /locus_tag="L2X49_003357"
     CDS             complement(8281..9816)
                     /gene="mdoG"
                     /locus_tag="L2X49_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBN4798390.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10210..11367
                     /gene="mdoC"
                     /locus_tag="L2X49_003358"
     CDS             10210..11367
                     /gene="mdoC"
                     /locus_tag="L2X49_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBN4798391.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11375..12856)
                     /gene="clsC"
                     /locus_tag="L2X49_003359"
     CDS             complement(11375..12856)
                     /gene="clsC"
                     /locus_tag="L2X49_003359"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBN4798392.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12798..13331)
                     /gene="ymdB"
                     /locus_tag="L2X49_003360"
     CDS             complement(12798..13331)
                     /gene="ymdB"
                     /locus_tag="L2X49_003360"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBN4798393.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13426..13737)
                     /locus_tag="L2X49_003361"
     CDS             complement(13426..13737)
                     /locus_tag="L2X49_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBN4798394.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13858..14190)
                     /gene="csgC"
                     /locus_tag="L2X49_003362"
     CDS             complement(13858..14190)
                     /gene="csgC"
                     /locus_tag="L2X49_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBN4798395.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14249..14704)
                     /gene="csgA"
                     /locus_tag="L2X49_003363"
     CDS             complement(14249..14704)
                     /gene="csgA"
                     /locus_tag="L2X49_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBN4798396.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14745..15200)
                     /gene="csgB"
                     /locus_tag="L2X49_003364"
     CDS             complement(14745..15200)
                     /gene="csgB"
                     /locus_tag="L2X49_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBN4798397.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3789 a         3920 c         3638 g         3892 t
ORIGIN      
        1 tattgaaata aaaataaata tcattcaatt tgttattttt gtgttgcaat tcattctccg
       61 acacgccatt ttctttctct cgcaaattcg tattggatgc acttatgtca ttcacaaata
      121 cccctgaacg ctatggtgtt atatctgcgg ccttccattg gctaagcgca attattgtct
      181 acggcatgtt tgctttaggt ctgtggatgg tcacgctcag ttattacgat ggctggtatc
      241 acaaagcacc cgaactgcat aaaagcatcg gtattttgtt aatgatgggg ctggttattc
      301 gcgttctgtg gcgtgtcata tctccaccgc cgggtccgct gccgagttat tcgcccatga
      361 ctcgtcttgc cgcaaaggct ggacatctcg cattgtacct attgctgttc gccatcggca
      421 tcagcggcta tctgatctca tctgccgatg gtaaaccgat cagcgttttt ggctggtttg
      481 acgtccccgc gacccttgcc gacgctggcg cacaggcaga ctttgccggt gccctgcatt
      541 tttggctcgc gtggagcgtc gtagtactgt ccattatgca cggatttatg gccctgaagc
      601 atcatttcat cgataaagac gacactctga agcgcatgct gggaaagtcg tcatctgact
      661 atggagtata aaaaatgaaa aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct
      721 ctgccggttc agcggttgcc gccgattaca aaattgacaa agaaggtcag cacgcctttg
      781 ttaatttccg catccagcac cttggctata gctggttata cggcaccttt aaagatttcg
      841 acggtacttt tacctttgac gaaaaaaatc cggctgccga taaagtgaat gtgacaatta
      901 acaccaccag cgtcgatact aatcacgccg aacgcgataa acatcttcgc agtgcagatt
      961 tcctcaatac cgcaaaatat ccacaggcaa cattcacctc caccagcgtg aagaaagacg
     1021 gtgacgaact ggatattacc ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc
     1081 tggaagcgaa attaattggt cagggcgacg acccatgggg tggtaaacgt gcaggcttcg
     1141 aggccgaagg caaaattaag ctcaaggact tcaatatcaa gacagattta ggtccagctt
     1201 ctcaggaagt ggatctgatt atttcagtgg aaggcgtaca gcagaagtaa gagtgaaatg
     1261 ttgccggatg gcacgcatcc ggcaatgata tttattccgt tggatcagga atgcccagtg
     1321 ttgtattcag acgtccacga gacttattaa agatcttatt gccattttca cgtcctgccc
     1381 gacggcgtcg ctgttcttct ggcggtaacg cgctttcttc gcagcaaatc tcactacaac
     1441 aacctttgta tttttccgca catactggac actgaataaa cagcaggtgg cagccatcat
     1501 ttttacagtt ggtatggctg tcgcacggcg cgccgcactg gtggcaatgc gcgataatct
     1561 catccgatat acgttcgccc atccgctcgt caaaaacaaa atttttgcca ataaaacgca
     1621 ccggcaagcc ctgctcgcgc gccttacggg cgtattcaat aattccgccc tcgatatgcc
     1681 agactttgtt gaatccgtta tgtttcatcc aggcactggc cttttcacaa cgaatgccgc
     1741 cggtgcagta catgacgatt tttttatctt tatgtgcctg catcatctcg actgcttttg
     1801 gcagctgctc acggaaggta tctgccggaa tttcgagcgc gttttcaaag tgccccactt
     1861 catactcata gtggttacgc atgtcgataa atagtgcatc gggatcgtca agcatggcgt
     1921 tcacttccgc cgcttgcaga tactcaccaa cattgctggc atcaaagtga ggatcgtcaa
     1981 taccgtcggc aacaatgcga tcgcgtacct tcatgcgcag tacccagaaa gatttcccgt
     2041 catcatccaa cgcgatattc aggcgtaaac cctctaaagc cgggtcgaag gcatagagct
     2101 gcgcgcgaaa tgtttcaaca ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat
     2161 gcgccagata cactcgccca aaaacattca gcgcggtaaa cagctgatat aaagcgtcac
     2221 gggtcgcctt aggatcggcg atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt
     2281 cgctctcagc caacattttg gcttttagcg cgtcgttgga aatgcggttg tgtaacactg
     2341 gcatggtgta cggttcctgc gagatgggaa agtaaaaatc cgcggcatga tatagcaatt
     2401 atcgataatt aacatccaca cattttacgc tacatttgcg cattaaaaat tatttgttat
     2461 ttacaagcgc ggcaatttcg cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa
     2521 tgcgacaata catacaattg cccgaatagg ttgaaaaaca ggattgatat gacgaatcta
     2581 cccaagttct ccaccgcact gcttcatccg cgttattggt taacctggtt gggtattggc
     2641 gtactttggt tagtcgtgca attgccctac ccggttatct accgcctcgg ttgtggatta
     2701 ggaaaactgg cgttacgttt tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa
     2761 ctgtgcttcc cggaaatgag cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa
     2821 tccgttggca tgggcctgat ggaaaccggc atggcgtggt tctggccgga ccgccgaatc
     2881 gcccgctgga cggaagtgat cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc
     2941 ggcatcctgt tagttggcat ccattttctg acactggagc tgggtgcgcg gcagtttggt
     3001 atgcaggaac cggggattgg cgtttaccgc ccgaacgata atccactgat tgactggcta
     3061 caaacctggg gccgtttgcg ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc
     3121 atgattaaag ccctgaaaaa aggcgaagtg gtctggtacg caccggatca tgattacggc
     3181 ccgcgctcaa gcgttttcgt cccgttattt gccgttgagc aggctgcgac cacgaccgga
     3241 acctggatgc tggcacggat gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag
     3301 ccagatggca aagggtatca attgattatg ctgccgccag agtgttctcc gccactggat
     3361 gatgccgaaa ctaccgccgc gtggatgaac aaagtggtcg aaaaatgcat catgatggca
     3421 ccagagcagt atatgtggtt acaccgtcgc tttaaaacac gcccggaagg cgttccttca
     3481 cgctattaaa tctcccctgc cggatgcttc agaatggcat ccggcattac cacagcaaat
     3541 ccccctgatt tagcgataaa agctctctgg attgcgcccc ctggaagtcg ggcgcataat
     3601 tagtgtgctt atcttttctt cttatgttca ccgcgcctgg cgcaccaaca gcggattgct
     3661 atgtcaccct gtgaaaatga cacccctata aactggaaac gaaacctgat cgtcgcctgg
     3721 ctaggctgtt ttcttaccgg tgccgccttc agtctggtaa tgcccttctt acccctctac
     3781 gttgagcagc ttggcgttac cggtcactcc gccctgaata tgtggtccgg tattgtcttc
     3841 agcattacat ttttattttc ggccatcgcc tcaccgtttt ggggtggact cgccgaccgt
     3901 aaaggccgaa aactcatgct attacgctct gccctcggca tgggcatcgt gatggtgttg
     3961 atgggactgg cacaaaatat ctggcagttt ttgatcctac gggcgcttct tgggttactt
     4021 ggcggatttg tccccaacgc taatgctctt atcgccacac aagtaccgcg taataaaagc
     4081 ggctgggcgc tgggtacgct ctccacaggc ggcgttagtg gtgcgttgct cggcccaatg
     4141 gctggcggcc tgctcgccga tagctacggc ttacgtccgg tattctttat taccgccagt
     4201 gtgctcatac tctgcttttt cgtcaccctg ttctgcatca gagaaaaatt ccagccggtc
     4261 agcaaaaaag agatgctgca catgcgggaa gtggtgacat cacttaaaaa cccgaaactg
     4321 gtactcagcc tgtttgtcac cacgttaatc atccaggtgg cgacaggctc aattgccccc
     4381 attctgacgc tgtatgtccg cgaactggcg ggtaacgtca gtaacgtcgc ctttatcagt
     4441 ggcatgatcg cctcggtgcc aggcgtggcg gctctgctaa gcgcaccacg actcggcaaa
     4501 cttggcgatc gaatcggacc cgaaaagatc ctgattacag cgctgatctt ttctgtactg
     4561 ctgttgatcc caatgtctta cgttcagacg ccattgcaac ttgggatttt acgttttttg
     4621 ctcggtgccg ccgatggtgc actactcccc gccgtacaga cactgttggt ttacaactcg
     4681 agcaaccaga tcgccgggcg tatcttcagc tataaccaat cgtttcgtga tattggcaac
     4741 gttaccggac cattgatggg agcagcgatt tcagcgaact acggtttcag agcggtattt
     4801 ctcgtcaccg ctggcgtagt gttattcaac gcagtctatt catggaacag tctacgtcgt
     4861 cgtcgaatac cccaggtatc gaactgattt ttcgcctttc atacttgcaa aagcggagaa
     4921 tcagctatcc ttttccctga aacctcatca actcaaaggg agaatcgtga tgaccatgta
     4981 cgcaacgctt gaagaagcca ttgacgctgc acgcgaagaa tttcttgcag acaaccccgg
     5041 catcgacgct gaagatgcga atgtgcaaca gttcaatgcc caaaaatacg ttttgcagga
     5101 cggcgacatc atgtggcaag ttgagttttt tgccgacgaa ggggaagaag gtgaatgttt
     5161 acctatgctt agcggtgaag ccgcgcaaag tgtttttgat ggcgactatg atgagataga
     5221 gatacgccag gagtggcagg aagagaatac attacatgaa tgggacgagg gggaatttca
     5281 gcttgagcca ccgctggata ccgaggaagg acgcgcagca gctgatgagt gggatgaacg
     5341 ttaatcactc atacgggcca tgatgaatgt cgatcggcag cagtaaagta tcaaaaacca
     5401 gagagaatgg cagatcaagg atcgtgacat aacgccaggc ggagtcacgc acatcccatt
     5461 gcacaccggg ataatattgg ttgccatgcc cctgccccgg tatagtgcga ctaataatgc
     5521 tgccacagcc actcagcaag gtcaccatga tgctcaccac aattaaacgc atttgatacc
     5581 tctttcgtaa aaaaataccg gcataacgcc ggtattttta ttggctcgtt gtttttgccg
     5641 gatgcggcgt gaacgcctta tccgacccac aaaatcttgc aaaatcaata aattgcagga
     5701 acgatgtagg cctgataagc gtagcgcatc aggcaactac gtttttattg cgaagccgca
     5761 tccggtttac gcaatgccag tggattgagc tttatccctt cgtaataact cacccatgaa
     5821 gaatatctct ccggggaatt ccagacgcgg aaatgcagac gggccatcgt caccggatcg
     5881 cttagcagca ccaggcgacg atcgcgattc agcttctctg gcgtctcgtt cagcgcctgt
     5941 tcaacgtggc ggtcacgggc gatttccagc accttactgg cgcggtgacg cgcggtcgcc
     6001 attgcggttg ccagagcgtt aaatgacggg ttaaacactg cgtgcataaa gccatcatca
     6061 agggagcgtt gacgattcat ctcaaggaac cgatcggtat caaccagcac ctgcggcggc
     6121 gaatactctt ccgggatcag gaacagtttc cagcgtttgg tgcgcagacc aacggtggca
     6181 cggctggaaa tcaccgaaac aaacggtgac aggatcaacg agaagacaat cggtgccagc
     6241 cagaacagga aacgcagatc caaccacgcc ataccaacag cccacactaa ccctaacagc
     6301 agctgtgagc cgtggcgttt gaacgcttca ccccaggaag tggagtcatc atcacgctgc
     6361 ggtgaattcc acaccacttc ccagccaagg aacgcgctga cgacgaagac cgtatggaac
     6421 agcatgcgta ccggagccag cagcacggaa aacagcactt ccagcagcaa cgataatgta
     6481 acgcgccaga agccgccgta ttctttcgtt cctttgcacc agataagcaa aatgctcaat
     6541 aacttcggca ggaacaacag caccatggtc gaagcaaaaa gtgcaatcgc cagctcagga
     6601 cgccactgcg gccacaccgg gaacaactgc cgtggttgca ggaagtattg cggttcggtc
     6661 aacgcatgta ctacctgcaa tgcagtagag agcgcgagga acataaacca cagcggagcg
     6721 gagagataag acatcacgcc cgtcaggaat accgcacggt gaaccgggtg catacccttc
     6781 accaggaaca gacggaagtt catcaggtta ccgtggcacc agcggcggtc acgttttagc
     6841 tcatcaagca agttaggcgg caattcttca taagaacccg ggagatcgta agcaatccag
     6901 accccccaac ctgcacggcg catcaacgcc gcttccacga agtcatgtga caggattgaa
     6961 ccggcaaagg aaccttcgcc cggcagcgga gccagtgcgc agtgctcgat aaacggtttc
     7021 acgcggataa tcgcgttatg tccccagtag tgcgactcgc caagttgcca gaagtgcaaa
     7081 ccggctgtaa acagtggccc atacacgcgg gtcgcgaact gctgacagcg cgcatacagc
     7141 gtatccatac cggacgcttt cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc
     7201 atcaggcgca ccagcccgca caaacaatca ccggtcatta ccgagtcagc atccagcacc
     7261 accatgtagc tgtactggct gccccagcga cggcagaagt catcgatatt accgctttta
     7321 cgcttcacgc ggcgacggcg gcggcgatag aaaatctgac cttcgccacc gacttcagcg
     7381 ataagctcca tccaggcttt ttgctctgcg acgcagatat ccgggttata actgtcacta
     7441 agaatgtaga catcaaagtg tttggcattc ccggtggctt ttactgattc ccacgttgca
     7501 cgcaggccag caaaaacacg gttcacgtct tcgttacaga taggcatgat caacgccgtg
     7561 cgatgctccg ggtttaatgg ttcatcgcca actgttgacg cagatatact gtatttatcg
     7621 cgaccaataa gcagttgcag gaagcccatt aacgccgtcc agaatccggc ggacacccaa
     7681 cagaacagta ccgcaaagag gatcaggata ccggtttgca gcatataagg cagaagctgc
     7741 ataaaggaaa cccacaaatc ctgaccaacc atatccatag gattaatcag cgcccaaccc
     7801 tgataaggaa gaatggtctt catataccag gtcgcgacga cagtttgcgc gagcgtcagg
     7861 atcaacagaa tgtaacggcg gatggtaccg acggtacgcc acttttgctc gctctcctgc
     7921 tcttctttgg tcaaacgagc cagatagcgc ggcgtgacat cgcgtccacg caggcgatcc
     7981 cagaaacggc ctaccgggtt ggtacgccac gggtcgggaa acatcgagga gcgttttgct
     8041 tctggcatcg ccttcagctg atcgcgccct tcgtcgtctt taattaactg tccatcagca
     8101 agtgaatctg gccaggcttg ttccagacgc gcctttaccg agccttgcgg ggaatcatcc
     8161 tcccgcgccc aggtgcggtg ttcggcatcc agcgcctgat gaacggcgcg gatatcagtc
     8221 ttcggcaatg ccgctttctc gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc
     8281 ttattcattg gcaggtaact ggtagctcca ggtttcactc aacgtctgat cggcattcac
     8341 cagcgcagca cgcatttcag tggttttctt ggcatctttc actttcacac gcatcaccag
     8401 acgccagcct ttggtaaccg ggttatagcg caccgtgctt tcaactatct caccattatc
     8461 accaatgctg gtttgcgctg tgaccggggt atcctctggc agttttttca tctctgcgcc
     8521 ggtaaaatcg accacaaagg cgatagtacc gtcaggctgg cgaatcaggt tcgactgctt
     8581 cacatccccc gttgaacgac gcgtttgttg cacccatgcg ttatctggcg catgcagttt
     8641 gtcttcatca cggctgaagg tgatggtgta tttaaagttc atctctttac ccggctccgg
     8701 cagctgatcc ggcgtccagt aagcgacgat gttatcgttg gtttcatcgt tggttggaat
     8761 ttccaccagc tcaacgctgc ctttacccca ctcccctttc ggagtcaccc atgcgcttgg
     8821 acgaagatcg taacgatcat cgagatcttc aaagcgggag aaatcacgac cgcgctgcaa
     8881 cagaccgaag ccttgcgggt tctccatcga gaagctgctg accgctaaat gtttcgggtt
     8941 attcaacgga cgccagatcc attcgccatt accggcatgg atagacagac cgttagagtc
     9001 gtgcaactcc ggacgatagt tatttgcagg cgacggttgg ttcggcccaa acaggaacat
     9061 actggttaac ggtgcaaccc ccagtttgcc gactttatcg cgcagataga ttttcgactg
     9121 cacatccaca accgtgtcac gccctggcat aaccacgaat ttgtaagcac ctgtcgcgcg
     9181 cggcgagtca agcaatgcat aaatggttaa acgtttatca gtcggttttg gacgctcgat
     9241 ccagaactct ttgaagcgtg gaaattcttc acccgatggc aaggcggtat caattgccag
     9301 gccgcgggca gaaaggccat aaacctgacc tgcaccaatc acgcggaaat agctggcccc
     9361 gagcatgctg acgatttcat cgtttttatc tttgctgttg atcgggtaaa gcactttaaa
     9421 accggcaaaa ccaaggtctt ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa
     9481 ataatccggg ctgtatttga ttcgtttgac tgcggtggca gtcacttcat ttattttgac
     9541 cggggtatcg aagtacatac cctgatggta gaactcgagt ttgaatgggg tcttcagatt
     9601 gttccagtac gctttgtcat gattaaactg gatctgctga tagtccgcgt atttcatatc
     9661 gcggaaaacg gagggcaagt tgcttttggg cgcctcatag cctttcccgg ctaaggattg
     9721 agcttgcttt gcgacatcat caatactgaa agcccagctt gaagatgtat acagggttaa
     9781 cattactgca gcactcaacc aacgcatttt catcatttgt agtttatgtt tcataattag
     9841 taagcacttc cccctttgtg tgcttatatc gatccgatct attttaatgg ataaccagcc
     9901 attccgacaa cagaatcccg gtatcgttca gcacgctggc tcatggatta agcacctgat
     9961 tgcaaccgtt ttcactttcc gtgcttctcc taaagacaga agcatcgact tcgtgtaggg
    10021 tttgccagca attttattta tcaaccgtct tacagacgct tccattctgg ttagtggtag
    10081 cgatgatgct atgcataaaa accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc
    10141 tatggcaaca agcctaaagg cataacccga cagggataag acgaaaaatc cgagattagt
    10201 taaccatata tgaatccagt acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc
    10261 tggctgatgt tgttagggat cccttttcat atttctttaa tctattcgag ccatacatgg
    10321 catgtgaata gcgccgaacc atcattgtgg ctgacccttt ttaatgactt catccactcg
    10381 ttccgcatgc aggtattttt cgttatatcc ggctactttt cctacatgct ttttttacgc
    10441 tatcccttga aaaaatggtg gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca
    10501 gccatccccc tactgacatt accgcaattt attatgctgc aatatgtcaa aggaaaagcg
    10561 gaaagttggc ctgggctgtc attgtatgac aaatataata cgttggcctg ggaattaata
    10621 tcacacctgt ggtttttact ggtgttagta gtcatgacga cgctgtgcgt atggatattt
    10681 aagcgcatca gaaataattt agaaaattct gataaaacga ataaaaaatt ctcgatggta
    10741 aaactatcgg tgattttttt atgcctcggc atcggttatg cggtaataag aagaacgatt
    10801 tttattgtgt atccgcccat tctgagtaat ggcatgttca attttattgt catgcaaacg
    10861 ctgttttatt tgccgttctt tatcctcggc gcactggctt tcattttccc tcatcttaaa
    10921 gccttgttta ccacgccgtc tcgtggctgt acccttgcag cagcattggc gtttgtcgct
    10981 tatttactca accagcgcta tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg
    11041 atcaccatgg tcctcggtct gtggatggtg aatgtggtct tctcctttgg ccaccgtttg
    11101 cttaacttcc agtcagcgcg ggtgacttat tttgttaacg catcgctgtt tatctatctg
    11161 gttcaccacc cgttaacgct gtttttcggc gcatacatta caccgcacat cacctccaac
    11221 tggcttggtt ttctctgtgg cctgatattt gtagtaggga ttgcgataat tctgtatgaa
    11281 attcatttgc gcatcccgtt actgaagttt ttgttctctg gtaaaccggt tgttaagcgt
    11341 gagaacgata aagcaccagc ccgttaagcc acatttacaa taaccattcc acgggcaata
    11401 tcgacgccag tctgaccata acccgcttcc agaaactggt ggcgggttct tttttgagaa
    11461 taatctcttt ctttgcatga cgatcaaccc agttgatccg tccccacctg tccagacgga
    11521 gctgccaggc cgcatcatac tggctctgaa taaagcgttt atcaattaac tgtgccagcg
    11581 tttcgctctc tatcacgaag cccatttcag tattgagcaa tgttgaacgc ggatcgaaat
    11641 tgaaagagcc gataaacacc gttttaccat cgatgctaaa ggttttagca tgcaggctgg
    11701 ctccggaatt accggttatg ccgcgatcgt gtaacgtact actttgttca cgcgtcggct
    11761 tgagttcata taattccacg ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg
    11821 catggacgac agcaacatcg ttagcggcaa gagaattggt taggatcgca atctttaccc
    11881 cttttctcac catccgtaag agttgcgcca cacctgcgcg tgtcggtaca aaataggaag
    11941 agataatatc gatgcgttca ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca
    12001 gtgaatgccg ttttgccttg ccctcccctt tcgccggatc atcacttaat aaacgtgtct
    12061 tcgcccagat aagcggcaat gttccatcaa ccagatgatt tataaatgga ctggattcca
    12121 ttttgcgtaa ataacgatgc gtcatggcat cgttatgcca ggaggcgggt aactcgatgc
    12181 gatccgccat ttcaccctcc gggacatcca gcacctgctg taagggtgaa accgatttgc
    12241 aataccagta gcgggcgaaa tcatcggcaa cgtcctctac cacgggtcct attgccatga
    12301 catctaaatc cgaaaaaagt ggctcctccc ctgctccaaa ataggcatca ccaatatttc
    12361 gtcctcccac cagggtcacc acgccatcga cagtgaaact tttattgtgc atacggcgat
    12421 taagacggga aaagtcggtg atataaccaa gcggacgcag caagcgaaac gagaaaggat
    12481 taaaaagccg gacttcaatg cgtggatgac tgtcaagcaa gcgtaaaatg tcgtcaagtc
    12541 cgggcgtatt gttgtcgtcc agcaacaaac ggacgcgaac gccacgcttt gctgcggcta
    12601 acagggcgga aaacagtaac cgacccgaca tatcgtcctg ccagatgtaa tactgaacat
    12661 cgagcgtatg ctctgccatt tcggccagac gataccgggc ggcaaacgca tccagacttt
    12721 tctccagagg aaaaaggcca cactgaccgg gatgttgcga acacagtggc agcaccgcgc
    12781 tcgccagccg gggcaaatca ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg
    12841 gcgttttctt catcataaca gacaaagtat acctgttcgg gtaaagcgtg acgggtaata
    12901 aattctgaaa cggtttttac tgcgatttca gccgctgccg cacgagggta accataaacc
    12961 ccagtactga ttgcaggaaa agccactgac gtatagctgt ttgccgccac cagtcgtagg
    13021 ctattgagat aggcatcctg caaaagctgg tcttcgtttt gttcaccacc acgccagact
    13081 ggcccgacgg tgtgcactac ggctttagcg ggaagagcgc ctgcaagcgt aataacggca
    13141 tgccccgtag ggcaatcgcc ctgctgttgc ctgactttta aacaggcatc cagtagggcc
    13201 ggacccgctg cgcgatgaat ggccccatcg acgccgccac ctcccattaa tgaaggatta
    13261 gccgcattca caatcacatc aacggccagt ttggtaatat caccctgcac aacatgaata
    13321 cgcgttttca taacgcctcc ttacaccttc ttgtttctta agtgtacagc aggctttttg
    13381 gttaaatcgc atttttctac tcagcaacgt gagttgttca gaaacttagt tatattcgat
    13441 gttcattaca gccagctttt tctgtttatc aagatactgc acttttactg aggctatttg
    13501 cgcgtttttg acattgccgc tcattaacgc tttgctggaa taggttttgc ccggaacaga
    13561 accattctgt gggcagttca tttctacggg tgatgactgg gtgctgacgt cacatgccgg
    13621 ttccacaatt tggccataaa aatggatcac gcccccttgc gcggtgcttc ctgcgatggc
    13681 aataaaagga aaaaacaaac tgccgagcat aagagagttt aaaaaaggtc tgaacattta
    13741 ttctatttcc tcaatgatta gtcatccttg agggttgtgt tatccatact ttcaggttgg
    13801 ctgattcgca gcagaccatt ctctccagat tcatcttatg ctcgatattt caacaaatta
    13861 agacttttct gaagagggcg gccattgttg tgataaatga agtgactgtc catcagaaac
    13921 agtaacaact attttcaccc gatcgtccgg ggaaatattt aaactcaact tcgtcaaagc
    13981 aatgggttga ttagcaggca atgaaagggt cttttcttgc ttcgtctgac tttgccctga
    14041 actgccttcg cgcagggaca atatttgtac tctgcacaga caagattgag taagagtgac
    14101 ttcaggaata atggtataca catccccttg ctgggtcgta ttaaaggtta tctgactgga
    14161 aagtgccgca aggagtaata acgcattcat attcttctcc cgaaaaaaaa cagggcttgc
    14221 gccctgtttc tgtaatacaa atgatgtatt agtactgatg agcggtcgcg ttgttaccaa
    14281 agccaacctg agtcacgttg acggaggagt tagatgcagt ctggtcaact gcagcaccgt
    14341 tgccaccacc gaactgttta accgtcattt cagaattttt gccgttccac tgatcaagag
    14401 tagcgctgtt accgaagcca cgttgggtca gatcgattga gctgtcatcc gagccctgac
    14461 caacatctgc accattaccg ccgccatgct gggtaatagt caagtcagag ttacgggcat
    14521 cagtttgcag agcaagtgca gagttaccgc caccgtactg gtaaatgttc agctcagaat
    14581 ttgggccgct attattaccg ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa
    14641 cacctgccag agcgctaccg gagaatacga ttgctgcaat tgctgctact tttaaaagtt
    14701 tcatgtaaaa cccccatcgg attgatttaa aagtcgaatg gaaattaacg ttgtgtcacg
    14761 cgaatagcca tttgcgactg tctctgcact acaattgccg ttttttgagt accatactgt
    14821 gtaatatttg ctttattacc agaacctttc tggataatca tcgcagtatt accataagca
    14881 ccttgcgaaa tactggcatc gttggcactg cccgcctgat caatatatgc aaggttataa
    14941 tctcctgtct ggtcaatctt tgcccggttg ctactacctt cttgcgcaac aaccgccaaa
    15001 agttttgagc ctccctgccg taactgagca ctattattcg tcccagcttg accaattatg
    15061 gctgcctgat taaatgaaga cttactcaat tcatttaccg cgaagttata ttctgaatta
    15121 gctaaatcat aacctgctgc ggctgcaatc ccaggcgcac ccagtattgt taacatcata
    15181 aataacaatt tgtttttcat gttgtcaccc tggacctggt cgtacattta agaaattaa
//
LOCUS       DAFNBJ010000111        15184 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000111 DAFNBJ010000000
VERSION     DAFNBJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.111"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(1..1757)
                     /locus_tag="L2X49_003365"
                     /pseudo
     CDS             complement(1..1757)
                     /locus_tag="L2X49_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2281..4209
                     /gene="thrS"
                     /locus_tag="L2X49_003366"
     CDS             2281..4209
                     /gene="thrS"
                     /locus_tag="L2X49_003366"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBN4798398.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITTKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4213..4755
                     /gene="infC"
                     /locus_tag="L2X49_003367"
     CDS             4213..4755
                     /gene="infC"
                     /locus_tag="L2X49_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBN4798399.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4852..5049
                     /gene="rpmI"
                     /locus_tag="L2X49_003368"
     CDS             4852..5049
                     /gene="rpmI"
                     /locus_tag="L2X49_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBN4798400.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5102..5458
                     /gene="rplT"
                     /locus_tag="L2X49_003369"
     CDS             5102..5458
                     /gene="rplT"
                     /locus_tag="L2X49_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBN4798401.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5575..5699
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5581..5625
                     /gene="pheM"
                     /locus_tag="L2X49_003370"
     CDS             5581..5625
                     /gene="pheM"
                     /locus_tag="L2X49_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBN4798402.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5909..6892
                     /gene="pheS"
                     /locus_tag="L2X49_003371"
     CDS             5909..6892
                     /gene="pheS"
                     /locus_tag="L2X49_003371"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBN4798403.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6907..9294
                     /gene="pheT"
                     /locus_tag="L2X49_003372"
     CDS             6907..9294
                     /gene="pheT"
                     /locus_tag="L2X49_003372"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBN4798404.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9299..9598
                     /gene="ihfA"
                     /locus_tag="L2X49_003373"
     CDS             9299..9598
                     /gene="ihfA"
                     /locus_tag="L2X49_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBN4798405.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9699..10679
                     /gene="btuC"
                     /locus_tag="L2X49_003374"
     CDS             9699..10679
                     /gene="btuC"
                     /locus_tag="L2X49_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBN4798406.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            10742..11292
                     /gene="btuE"
                     /locus_tag="L2X49_003375"
                     /pseudo
     CDS             10742..11292
                     /gene="btuE"
                     /locus_tag="L2X49_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
     gene            11292..12041
                     /gene="btuD"
                     /locus_tag="L2X49_003376"
     CDS             11292..12041
                     /gene="btuD"
                     /locus_tag="L2X49_003376"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBN4798407.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12119..12583
                     /locus_tag="L2X49_003377"
     CDS             12119..12583
                     /locus_tag="L2X49_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HBN4798408.1"
                     /translation="MRFCLILITALLLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12830..13543
                     /gene="ydiV"
                     /locus_tag="L2X49_003378"
     CDS             12830..13543
                     /gene="ydiV"
                     /locus_tag="L2X49_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBN4798409.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13606..15042
                     /locus_tag="L2X49_003379"
     CDS             13606..15042
                     /locus_tag="L2X49_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBN4798410.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGERMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(15046..>15184)
                     /gene="hemP"
                     /locus_tag="L2X49_003380"
     CDS             complement(15046..>15184)
                     /gene="hemP"
                     /locus_tag="L2X49_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBN4798411.1"
                     /translation="TASPQPIRRISSQTLLGPDGKLIIDHDGQEYLLRKTQAGKLLLT
                     K"
BASE COUNT         3864 a         3454 c         3989 g         3877 t
ORIGIN      
        1 gaacagctga catgttttca tgatttttag cggccaacag tgtgaataat tgacgttgac
       61 taaaagaata tttttttgca aaagtactca gtgttgatat ataagataac acgacgtctt
      121 tattaccctt gctcatggcg atgtataaag caggggttcc atgtgctagc tttgctcaac
      181 ttgtatttaa aggcgatgcc attaatttta gagaggaacc gtgtgacaca caaagggtga
      241 ttattttcca ttgcggcgta taatcctggc tcaccatcat gtattttcat ggctaaaata
      301 ctcatcacca gttttttgtt taaaagctct tgtgctattg gagtggttaa tgcattcaga
      361 aagatatcga ttatttcatc gaatccatac aacatggaga tataaagtcc aggtgtttcg
      421 ttagcacttt tagtttgcag taatttaacc agattatctt catgaatgat gtgattatct
      481 accagtgact gaatttcttg cataaatatt ttcacgttgt cagtatgtcc gttcatcata
      541 gccaaaaata aagcaggtgt accaacgcta ttctgcgcag ttattatttc aatgatttct
      601 ttttcaggga gtatgggcaa ctgtttgaat aatgtaataa gctgttgagt ctggcctgta
      661 gccattatta acaacattgc cgatggatgt ataggggcaa atggcattcg tgatgcaata
      721 cccgcgaaaa tagcccctgg aactcccaca ggtaataggg gcagaatagc aattgcacag
      781 ttgcgaacat acctatcagc ccaggtctga gcatcgggac ctgaactgat gaacttatca
      841 agcgaaagaa aggcacgtgc tgtcctgtgg gtgtctctga cggcaacgtt ggtatcgttc
      901 gggtcgtaaa cggacacaac atagtttaat tgtccataca ctgactgctt tattcgtagc
      961 cttactgaca aagtatgatt gtctatatat aaaatcgcgg acatatgact gacattgttt
     1021 gccatcattc gacaaaagaa aatatctaaa gcgaagccaa aatccttact cgcgataacg
     1081 cgactccctg caggaggatg gcgaagtaat gctctgaact ccgcatcccg gactaagggg
     1141 atatgctgct gtatggattc aacggaggag taatgtttaa tcaggttgac tttgcctttt
     1201 ccatttctca atgaatcaag ggcgtattgc aatgacagat ggcgacaaaa aatagcgtca
     1261 gaaggagatt gcaaaaaaca tgcactacca ttgagattta ctgttggttg tatagttttt
     1321 tctctatgga tttttttaga cagtttcata caatggttac gatcttcggc agttaactgt
     1381 tcctgctcaa tataatccag tatgcttcgc aaatggttag ccttaatagg cgtggtttcc
     1441 agtaataagg aaatgaaatc ttttttatag tgtaatgtaa tgagtatatt ttctgcatgg
     1501 atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt ttgtgatatt
     1561 tttcgacagt ttgcggtaaa ttcctgaata acacagtatg acagcatcga acttaagtca
     1621 gaggtatcaa gtagggtgat gagctggcgc gcaaatttat caataggctc tgatgaaggt
     1681 gttggtatgt cgctatcgat gtgcaatcta tgaggagact tataatgagt tctgataatg
     1741 atatcgtttt gactcataat ctattcgcct tatatatatc tggaggtttt gaggatgatg
     1801 attaattatt atcgccagaa cgaaatgtgc acgaaaaatt gttataagaa ttgtgtcact
     1861 tttatcattg tgatatgacc acttcgtcat taatctcatg agcgtttaaa atactaccgc
     1921 caaatcacgc aacgaacttt gctggattca gtttatgctg ctgtaaatcc gctcgcagta
     1981 aacctttcag acgcaccgtg atgttatcag tggttcttta agcgttttgc tggtgtactc
     2041 actacaaacg aattgcgaat cgaatcaatg tgaaacggaa aggtacaatc tccctttttt
     2101 tagtgttaac atcgctcaac cgggtggaag gtgagaggtt aatgtctttc atttgtgtac
     2161 ctgattgcga accaatttag catttgttgg ctagatggtt tcgcaatgaa ctgttaataa
     2221 acaaattttt ctttgtatgt gatctttcgt gtgggtcacc actgcaaata aggatataaa
     2281 atgcctgtta taactcttcc tgatggcagc caacgccatt acgaccacgc tgtaagcccc
     2341 atggatgttg cgctggacat tggtccaggt ctggcgaaag cctgtatcgc agggcgcgtt
     2401 aatggcgaac tggttgatgc ttgcgatctg attgaaaacg acgcacaact gtccatcatt
     2461 accaccaaag acgaagacgg tctggagatc attcgtcact cctgtgcgca cctgttaggg
     2521 cacgcgatta aacaactttg gccgcatacc aaaatggcaa tcggcccggt tattgacaac
     2581 ggtttttatt acgacgttga tcttgaccgc acgttaaccc aggaagatgt cgaagcactc
     2641 gagaagcgga tgcatgagct tgctgagaaa aactacgacg tcattaagaa gaaagtcagc
     2701 tggcacgaag cgcgtgaaac tttcgccaac cgtggggaga gctacaaagt ctccattctt
     2761 gacgaaaaca tcgcccatga tgacaagcca ggtctgtact tccatgaaga atatgtcgat
     2821 atgtgccgcg gtccgcacgt accgaacatg cgtttctgcc atcatttcaa actaatgaaa
     2881 acggcagggg cttactggcg tggcgacagc aacaacaaaa tgttgcaacg tatttacggt
     2941 acggcgtggg cagacaaaaa agcgcttaac gcttacctgc agcgcctgga agaagccgcg
     3001 aaacgcgacc accgtaaaat cggtaaacag ctcgacctgt accatatgca ggaagaagcg
     3061 ccgggtatgg tattctggca caacgacggc tggaccatct tccgtgaact ggaagtgttt
     3121 gttcgttcta aactgaaaga gtaccagtat caggaagtta aaggtccgtt catgatggac
     3181 cgtgtcctgt gggaaaaaac cggtcactgg gacaactaca aagatgcaat gttcaccacg
     3241 tcttctgaga accgtgaata ctgcattaag ccgatgaact gcccgggtca cgtacaaatt
     3301 ttcaaccagg ggctgaagtc ttatcgcgat ctgccgctgc gtatggccga gtttggtagc
     3361 tgccaccgta atgagccgtc aggttcgctg catggcctga tgcgcgtgcg tggatttacc
     3421 caggatgacg cgcatatctt ctgtactgaa gaacaaattc gcgatgaagt taacggatgt
     3481 atccgtttag tctatgatat gtacagcact tttgggttcg agaagatcgt cgtcaaactc
     3541 tccactcgtc ctgaaaaacg tattggcagt gatgaaatgt gggatcgtgc tgaggcggac
     3601 ctggcggttg cgctggaaga aaacaacatc ccgtttgaat atcaactggg tgaaggcgct
     3661 ttctacggtc cgaaaattga atttaccctg tatgactgcc tcgatcgtgc atggcagtgc
     3721 ggtacagtac agctggactt ctctttgcct tctcgtctga gcgcttctta tgtaggcgaa
     3781 gacaatgaac gtaaagtacc ggtaatgatt caccgcgcaa ttctggggtc gatggaacgt
     3841 ttcatcggta tcctgaccga agaattcgct ggtttcttcc cgacctggct tgcgccagtt
     3901 caggttgtta tcatgaatat taccgattca cagtctgaat acgttaacga attgacgcaa
     3961 aaactatcaa atgcgggcat tcgtgttaaa gcagacttga gaaatgagaa gattggcttt
     4021 aaaatccgcg agcacacttt gcgtcgcgtc ccatatatgc tggtctgtgg tgataaagag
     4081 gtggaatcag gcaaagttgc cgttcgcacc cgccgtggta aagacctggg aagcatggac
     4141 gtaaatgaag tgatcgagaa gctgcaacaa gagattcgca gccgcagtct taaacaattg
     4201 gaggaataag gtattaaagg cggaaaacga gttcaaacgg cgcgccctaa ccgtatcaat
     4261 ggcgaaattc gcgcccagga agttcgctta acaggtctgg aaggcgagca gcttggtatt
     4321 gtgagtctga gagaagctct ggagaaagca gaagaagccg gagtagactt agtcgagatc
     4381 agccctaacg ccgagccgcc ggtttgtcgt ataatggatt acggcaaatt cctctatgaa
     4441 aagagcaagt cttctaagga acagaagaaa aagcaaaaag ttatccaggt taaggaaatt
     4501 aaattccgtc ctggtacaga tgaaggtgac tatcaggtaa aactccgcag cctgattcgc
     4561 tttctcgaag agggtgataa agccaaaatc acgctgcgtt tccgcggtcg tgagatggcg
     4621 caccagcaaa tcggtatgga agtgcttaat cgcgtgaaag acgatttgca agaactggca
     4681 gtggtcgaat ccttcccaac gaagatcgaa ggccgccaga tgatcatggt gctcgctcct
     4741 aagaagaaac agtaaggcct tcaagtagca ctgtctgtgg agccttcggg cttcacaggc
     4801 tttgttcgcc tttgtttcgt ttattaacaa tgcgaagtgg aagttattaa aatgccaaaa
     4861 attaagaccg tacgcggtgc tgctaagcgc ttcaaaaaaa ccggtaaagg tggttttaag
     4921 cacaagcacg ctaacctgcg tcacattctg accaaaaaag cgaccaaacg taaacgtcac
     4981 ctgcgtccga aagccatggt ttccaaaggc gatctgggcc tggtaatcgc gtgcctgccg
     5041 tacgcataag ccgttaacgt ttttaacttt ttaattagaa tatagataca ggagagcaca
     5101 tatggctcgc gtaaaacgtg gtgttattgc acgcgcacgt cacaagaaaa ttttgaaaca
     5161 agctaaaggc tactacggtg cgcgttctcg cgtataccgc gttgccttcc aggctgttat
     5221 caaagctggt cagtatgctt accgtgaccg tcgtcaacgt aagcgtcagt tccgtcaact
     5281 gtggattgcg cgtatcaacg cagcagcacg tcagaacggt atttcttaca gcaaattcat
     5341 caatggcctg aaaaaagcct ctgttgaaat cgaccgtaag atcctggctg atatcgcagt
     5401 attcgacaaa gtagcgttca ccgctctggt tgaaaaagcg aaagcagctc tggcataagc
     5461 cagttgaaag agggagctag tctccctctt ttcgtttcaa cgccatcaaa acattgactt
     5521 ttatcgccgt agccttttca ataaaggtct tttgaagagt accaaaaggt aacgcaagca
     5581 atgaatgctg ctattttccg cttctttttt tactttagca cctgaatcca ggaggctagc
     5641 gcgtgagaag agaaacggaa aacagcgcct gaaagcctcc cagtggaggc tttttttgta
     5701 tgcgcgtttt gaaaaataaa aagcctgtct catcaggtgt aattgtcgca gccagtctgg
     5761 acacggccag cgcggagaaa ccggagcgta tttaagtacg tgagaatttc gagcactgcc
     5821 cgggaccaaa atggcaagta aaatagcctg atgggatagg ctctaagtcc aacgaaccag
     5881 tgtcaccact gacacaatga ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc
     5941 gaaggcggcc attagccagg cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata
     6001 tttgggtaaa aaagggcact taacccttca gatgacgacc ctgcgtgagc tgccgccaga
     6061 agagcgtccg gcagctggtg cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct
     6121 gaatgcgcgt aaagcggaac tggaaagcgc agcgctgaat gcgcgtctgg cggcggaaac
     6181 gattgatgtc tccctgccag gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg
     6241 taccatcgac cgtatcgaaa gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc
     6301 ggaaatcgaa gacgattatc ataacttcga tgctctgaac attcctggtc accacccggc
     6361 gcgcgctgac cacgacactt tctggtttga cactacccgc ctgctgcgta cccagacctc
     6421 tggcgtacag atccgcacca tgaaagccca gcagccaccg attcgtatca tcgcgcctgg
     6481 ccgcgtttat cgtaacgact acgaccagac tcacacgccg atgttccatc agatggaagg
     6541 tctgattgtt gataccaaca tcagctttac caacctgaaa ggcactctgc acgatttcct
     6601 gcgtaacttc tttgaggaag atttgcagat tcgcttccgt ccttcctact tcccgtttac
     6661 cgagccttct gcagaagttg acgtcatggg taaaaacggt aaatggctgg aagtgctggg
     6721 ctgcgggatg gtgcatccga acgtgctgcg taacgttggc atcgacccgg aagtttactc
     6781 tggtttcgcc ttcgggatgg ggatggagcg tctgactatg ttgcgttacg gcgtcaccga
     6841 cctgcgttca ttcttcgaaa acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa
     6901 tagattatga aattcagtga actgtggtta cgcgaatggg tgaacccggc gattgatagc
     6961 gatgcgctgg cgaatcaaat cactatggcg ggcctggaag ttgacggtgt agaaccggtt
     7021 gccggtagct tccacggcgt ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac
     7081 gctgacaaac tgcgtgtgac aaaagtgaat gtcggcggcg atcgcctgct ggacatcgtc
     7141 tgtggtgcgc caaactgccg tcagggcctg cgtgtggcgg tagctaccat tggtgctgtt
     7201 ctgccgggtg atttcaaaat taaagcggcg aaactgcgtg gcgaaccgtc tgaagggatg
     7261 ctgtgctcct tttctgagct gggaatttct gacgatcaca gtggcattat cgaactgcct
     7321 gcggatgcgc cgattggcac cgacatccgc gaatacctga aactcgatga caacaccatc
     7381 gaaatcagcg taacgccaaa ccgtgccgac tgtttaggta tcattggtgt tgcgcgtgac
     7441 gttgccgtgc tgaatcagct gccgctggtt gaaccggaaa tcgttccggt tggtgcgacc
     7501 atcgacgaca cgctgccgat tacagtcgaa gcgccggaag cctgcccgcg ttatcttggc
     7561 cgtgtggtaa aaggcattaa cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg
     7621 cgtcgttgcg ggatccgttc tatcgatgca gttgttgacg tcaccaacta tgtactgctc
     7681 gaactgggcc agccgatgca cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg
     7741 cggatggcga aagagggcga aacgttggta ctgcttgatg gcactgaagc gaagctgaat
     7801 gctgacactc tggtcatcgc cgaccacaac aaggcgctgg cgatgggcgg catctttggt
     7861 ggcgaacact ctggcgtgaa tgacgaaacg caaaacgtct tgctggaatg cgctttcttc
     7921 agcccgctgt ctatcaccgg tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac
     7981 cgttatgagc gtggcgttga tccggcactg cagcacaaag cgatggaacg tgcgacccgt
     8041 ctgctgatcg acatctgcgg tggtgaggct ggtccggtaa ttgatatcac caacgaagca
     8101 acgctgccga agcgtgcaac catcacttta cgtcgtagca aactggatcg cctgatcggc
     8161 catcatattg cggatgagca ggtaactgac attctgcgtc gtctcggctg cgaagtgacc
     8221 gaaggcaaag acgagtggca ggcagttgcg ccgagctggc gtttcgatat ggagattgaa
     8281 gaagatctgg tcgaagaagt cgcgcgtgtt tacggctaca acaacatccc ggatgagccg
     8341 gtacaggcaa gcctgattat gggtactcac cgtgaagctg acctgtcgct caagcgcgtg
     8401 aaaacgctgc tcaacgacaa aggctatcag gaagtgatca cctacagctt cgttgatccg
     8461 aaagtgcagc agatgatcca tccaggcgtt gaagccttac tgctgccaag cccgatctct
     8521 gttgaaatgt cagcaatgcg tctttctctg tggaccggcc tgctggcaac cgtggtgtac
     8581 aaccagaacc gtcagcagaa ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca
     8641 gatactcagg caccgttggg cattcgtcag gatctgatgt tagccggtgt gatttgcggt
     8701 aaccgttacg aagagcactg gaacctggca aaagagaccg ttgatttcta tgatttgaaa
     8761 ggcgatcttg aatccgttct cgacctgacc ggtaaactga atgaggttga gttccgtgca
     8821 gaagcgaatc cggcactgca tccggggcaa tccgcagcga tttatctgaa aggtgaacgt
     8881 attggttttg ttggggttgt tcatcctgaa ctggaacgta aactggatct taacggtcgc
     8941 actctggtgt tcgaactgga gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc
     9001 gagatttctc gcttcccggc gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt
     9061 cccgcagcgg atattttatc cgaatgtaag aaagttggcg taaatcaggt agttggcgta
     9121 aacttatttg acgtgtaccg cggtaagggt gttgcggagg ggtataagag cctcgccata
     9181 agcctgatcc tgcaagatac cagccgtaca ctcgaagaag aggagattgc cgctaccgtc
     9241 gccaaatgtg tagaggcatt aaaagagcga ttccaggcat cattgaggga ttgaacctat
     9301 ggcgcttaca aaagctgaaa tgtcagaata tctgtttgat aagcttgggc ttagcaagcg
     9361 ggatgccaaa gaactggttg aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg
     9421 cgaacaggtg aaactctctg gttttggtaa cttcgatctg cgtgataaga atcaacgccc
     9481 gggacgtaac ccgaaaacgg gcgaggatat tcccattaca gcacggcgcg tggtgacctt
     9541 cagacccggg cagaagttaa aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc
     9601 tgatctaact aaaaaggccg ctctgcggcc ttttttcttt tcactgtcga agagtcaccg
     9661 taaaatcaac gccatgacac ttcagcagaa cggataccat gctgacactt gcccgccaac
     9721 aacagcgaca aaatattcgc tggttattat gcctgtcagt tttgatgctg ctggcgcttc
     9781 tcttaagcct ttgcgccggt gaacaatgga tctcgccagg tgactggttt actcctcgtg
     9841 gcgaactgtt cgtctggcaa attcgcctgc cacgtacgct ggctgtattg ctggttggtg
     9901 cggcgctggc tatatccggc gctgtaatgc aggcgttgtt tgaaaatcct ctggcagaac
     9961 ctggactact tggcgtctct aacggcgcag gcgtggggct tatcgccgcg gtattgcttg
    10021 ggcaagggca actccccaac tgggcgctag ggctgtgtgc gattgctggc gcgcttatca
    10081 tcactttaat actcttacgt ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg
    10141 ctggcgttgc attagggatt atctgtagcg cactaatgac gtgggctatc tacttttcca
    10201 cctctgttga tttacgtcag ctgatgtact ggatgatggg cggttttggc ggcgtagact
    10261 ggcggcaaag ctggctgatg ctggcattga tccccgtgtt gttgtggatc tgttgtcagt
    10321 ccaggccgat gaatatgtta gcacttggcg agatctcggc gcggcaactg ggtttacccc
    10381 tgtggttctg gcgcaatgtg ctggtggccg cgaccggctg gatggttggc gtcagtgtgg
    10441 cgctggcggg tgctatcggc tttattggtc tggtgatccc acatattctt cggttgtgtg
    10501 gtttaaccga tcatcgcgta ttacttcccg gctgcgcact ggcaggggcg agcgcattgc
    10561 tgctggccga tattgtagcg cgcctggcat tagctgccgc agagctgcct attggcgtgg
    10621 tcaccgcaac gttgggtgcg ccggtgttta tctggttatt gttaaaagca agacgttagc
    10681 cgcaaaaaga cggtctatga ttaaaagcta agatttttac tgaccacacc caggagaaac
    10741 gatgcaagat tccattctga cgaccgtagt gaaagatatc gacggtgaag tgaccacgct
    10801 ggagaagtac gctggtaatg tgctgttgat tgtcaatgtc gcctcaaagt gtggcttaac
    10861 gccgcaatat gagcagttgg agaatattca gaaagcctgg gccgatcgag gttttgtggt
    10921 gctgggattc ccgtgcaacc agtttctgga acaagaaccg ggcagcgatg aagagattaa
    10981 aacttactgt accaccacat ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa
    11041 tggcgaagga cgccatccgc tgtatcaaaa attgattgcc gcagcgccga ccgcagtcgc
    11101 gccggaagag agcggattct atgcccgtat ggtcagcaaa ggtcgtgcac cgctgtaccc
    11161 tgatgatatt ttatggaatt ttgagaattt ttagttggca gggacgggtt ggtcatccag
    11221 cgtttttccc cggatatgac gcctgaagat cccattgtga tggaaagcat taaactggcg
    11281 ttggcaaaat aatgtctatt gtgatgcagt tacaggatgt tgcggaaact acccgcctgg
    11341 ggccgctttc tggcgaggtt cgggctgggg agatcctgca cctggtgggg ccgaatggcg
    11401 cgggtaagag taccttactg gcgcgaatgg ccggaatgac cagcggtaag ggaagcattc
    11461 agttcgcggg gcaaccactg gaagcatggt ccgcaacaaa actcgcgctg catcgcgcct
    11521 atctttcaca acagcagacg ccgccgtttg caatgccggt ctggcactac ctgacactgc
    11581 atcagcacga taaaacgcgt accgaactac tgaatgatgt cgcaggggcg ctggctcttg
    11641 atgacaaact cggacgtagc accaatcaac tttccggcgg tgaatggcaa cgcgtacgtc
    11701 ttgctgcggt ggtgttgcaa atcacaccac aagccaatcc cgcaggccaa ttgctgcttc
    11761 ttgatgagcc gatgaacagt cttgatgttg cgcaacaaag tgcgttagac aaaattctga
    11821 gcgcgctgtg tcagcaagga ctggcgattg tgatgagcag tcacgatctc aaccacacat
    11881 tgcgtcatgc gcatcgggcg tggttgctaa aaggtggaaa aatgctggcc agtggacgca
    11941 gggaagaggt gctcacgccg ccaaatctgg cgcaggccta tgggatgaat tttcgccgtc
    12001 tggatatcga aggtcacaga atgctgattt cgaccatctg atttcattag tatcttgaaa
    12061 atcgggtaat tacgacgcta aattaatgca agaataaaaa acagaggaac cgtacggaat
    12121 gcgtttctgc cttattttga tcacagcact gcttctggcc gggtgtagcc accataaagc
    12181 accgccgcca aatgccagac tttctgattc gattaccgtt attgccggtt tgaacgacca
    12241 gctacaaagc tggcatggca cgccgtatcg ttatggtggc atgacgcggc gcggtgtgga
    12301 ctgttcggga tttgtggttg tgacgatgcg cgatcgtttc gatttgcagc tgccccgtga
    12361 aaccaaacaa caagcctcta ttggcacgca aattgataaa gacgagttgc tgccaggtga
    12421 cctggtcttt ttcaaaacgg ggtccggaca aaatggtttg catgtaggta tttacgatac
    12481 caacaaccaa tttatccacg cctctaccag caagggagtg atgcgttcct cacttgataa
    12541 tgtctattgg cagaaaaatt tctggcaggc gagacgaatc tagtatgtcg aaaaatggac
    12601 ggcgatgata catcgccgtc catttactcc tgaatacagt agtattttgc agacaaaaat
    12661 tccgtaaatg gattattcgt ttataatcag gataatctaa aaaaaggacc cctgaaaaag
    12721 tggttaacat ggaacaaatg aaagttactc cttctgtaat tattatgtct tatggcctca
    12781 tcccttttag ccggatactg aaaaacatcc ttcgagaggg acggttacca tgaagatttt
    12841 tttggagaat ctttatcatt ctgattgcta tttcctgccg ataagagaca accagcaagt
    12901 ccttgtcggc gttgagttaa ttacccattt ctccagtgaa gatggcacgg tgcgtatccc
    12961 gacgagtcgg gttatagcgc aacttaccga ggagcaacat tggcaattat tttcagagca
    13021 gttggaatta ctaaaatcat gccaacattt ttttattcaa cataaactct ttgcctggct
    13081 taatttaacg ccacaagtgg ctacattatt attagagagg gataattacg caggtgaact
    13141 attaaaatat ccgtttattg aactgttgat taatgagaat tacccgcatc tcaatgaagg
    13201 aaaagataat cgcggtcttt tatccttatc gcaagtgtat ccccttgtgt taggtaattt
    13261 gggagcaggc aatagcacaa tgaaagcggt ttttgatggt cttttcaccc gcgtcatgct
    13321 ggataaaagt tttattcagc aacaaatcac gcaccgctcc ttcgaaccgt ttattcgtgc
    13381 gatccaggcg caaatttccc cttgttgcaa ttgcatcatt gcgggaggca ttgatacggc
    13441 agaaatattg gcacagatta cccccttcga tttccatgct ctccagggat gcctgtggcc
    13501 tgccgttccc ataaatcaga taacgacgtt ggttcagcga taacccttct gtttgctggt
    13561 gtttaagacg agagtaaccg tctacactat caaacaggag gatctatgac cctgtctttt
    13621 gttacccgct ggcgcgatga attgccagaa acctatacag cactttcccc tacgccttta
    13681 aataatgccc ggctgatttg gcataatacc gaactggcta acacgctgag tattccatcg
    13741 tcgctgttta aaaatggcgc aggtgtctgg ggcggcgaag ccctactgcc tggtatgtca
    13801 ccactggcgc aggtttacag tggtcatcag ttcggcgtct gggcgggcca actgggtgat
    13861 gggcgcggca ttttactcgg cgaacaactg cttgctgatg gcactacaat ggactggcat
    13921 ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc ggttttacgt
    13981 tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat tccgacgacc
    14041 cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggc ggagccaggc
    14101 gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga acatttttac
    14161 taccgccgcg agtcggaaaa ggttcgtcag ttagctgact ttgctattcg tcattactgg
    14221 tcacatcttg aagatgatga ggacaaatac cgtctctggt ttagcgatgt tgtcgcacgt
    14281 accgcgtcgt taattgccca atggcagacg gtcggctttg ctcatggggt gatgaatacc
    14341 gacaacatgt cgctgctggg gctgacgctt gattacgggc cgtttggttt tcttgatgat
    14401 tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttacag ctttgataat
    14461 caacctgccg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc gccatttgtt
    14521 gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt gacgcattat
    14581 ggagaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga taacgcgcta
    14641 ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg cacattccgc
    14701 atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgatga gtttattgat
    14761 cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca agacgaggtt
    14821 agcgatagtg agcgtcagca gctgatgcaa agcgttaacc cagctctggt gttgcgcaac
    14881 tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgacgga attgcaccgc
    14941 ctgcatgagg cgttgcgaaa tcctttcagc gacagagctg atgactatgt cagtcgtcca
    15001 cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact tggtcaacag
    15061 cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat cgataatcag
    15121 tttgccatcc ggacctaaca gtgtctggct ggaaattcgc cgaataggct gcggggaagc
    15181 ggtc
//
LOCUS       DAFNBJ010000112        15167 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000112 DAFNBJ010000000
VERSION     DAFNBJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.112"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..500
                     /locus_tag="L2X49_003381"
     CDS             <1..500
                     /locus_tag="L2X49_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBN4798412.1"
                     /translation="LLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETKTVNII
                     SILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVGAA
                     AGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPNTK
                     CPEKS"
     gene            516..1055
                     /locus_tag="L2X49_003382"
     CDS             516..1055
                     /locus_tag="L2X49_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBN4798413.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQKQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1150..2727)
                     /gene="guaA"
                     /locus_tag="L2X49_003383"
     CDS             complement(1150..2727)
                     /gene="guaA"
                     /locus_tag="L2X49_003383"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBN4798414.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2796..4262)
                     /gene="guaB"
                     /locus_tag="L2X49_003384"
     CDS             complement(2796..4262)
                     /gene="guaB"
                     /locus_tag="L2X49_003384"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBN4798415.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4424..5794
                     /gene="xseA"
                     /locus_tag="L2X49_003385"
     CDS             4424..5794
                     /gene="xseA"
                     /locus_tag="L2X49_003385"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBN4798416.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5791..6006)
                     /locus_tag="L2X49_003386"
     CDS             complement(5791..6006)
                     /locus_tag="L2X49_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBN4798417.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6075..7547)
                     /gene="der"
                     /locus_tag="L2X49_003387"
     CDS             complement(6075..7547)
                     /gene="der"
                     /locus_tag="L2X49_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBN4798418.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7665..8843)
                     /gene="bamB"
                     /locus_tag="L2X49_003388"
     CDS             complement(7665..8843)
                     /gene="bamB"
                     /locus_tag="L2X49_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBN4798419.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8854..9474)
                     /gene="yfgM"
                     /locus_tag="L2X49_003389"
     CDS             complement(8854..9474)
                     /gene="yfgM"
                     /locus_tag="L2X49_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBN4798420.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9492..10766)
                     /gene="hisS"
                     /locus_tag="L2X49_003390"
     CDS             complement(9492..10766)
                     /gene="hisS"
                     /locus_tag="L2X49_003390"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBN4798421.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10877..11995)
                     /gene="ispG"
                     /locus_tag="L2X49_003391"
                     /gene_synonym="gcpE"
     CDS             complement(10877..11995)
                     /gene="ispG"
                     /locus_tag="L2X49_003391"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBN4798422.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12022..13035)
                     /gene="rodZ"
                     /locus_tag="L2X49_003392"
     CDS             complement(12022..13035)
                     /gene="rodZ"
                     /locus_tag="L2X49_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBN4798423.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13320..14474)
                     /gene="trmG/rlmN"
                     /locus_tag="L2X49_003393"
     CDS             complement(13320..14474)
                     /gene="trmG/rlmN"
                     /locus_tag="L2X49_003393"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBN4798424.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14624..15055)
                     /gene="ndk"
                     /locus_tag="L2X49_003394"
     CDS             complement(14624..15055)
                     /gene="ndk"
                     /locus_tag="L2X49_003394"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBN4798425.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
BASE COUNT         3670 a         4176 c         3769 g         3552 t
ORIGIN      
        1 gtcttctgcc tgtggcaatg ttagcgtcat tcactctggc aggatgccag tcaaatgctg
       61 acgatcatgc cgccgatgtt tatcaaaccg atcaactgaa taccaaacaa gaaactaaaa
      121 ccgttaatat tatttccatt cttcccgcaa aagttgccgt agacaactcc caaaataaac
      181 ggaacgcaca agccttcggc gcgcttattg gcgcagtcgc tggcggtgtt atcggccaca
      241 acgtcggttc tggcagcaat tccggaacga cggcaggggc agttggcggc ggagctgtag
      301 gcgcggcagc gggttctatg gtgaatgata aaaccttagt ggaaggtgtt tctttaacgt
      361 ataaggaagg caccaaagtg tatacctcca cccaggtggg taaagagtgc cagtttacga
      421 caggtttagc cgttgttatt accacgacgt ataacgaaac gcgtattcag ccaaatacca
      481 aatgtcctga aaagagctaa taatcaggag gagtcatgaa gaaagttttt ctttgcgcca
      541 tcttggcctc cttaagctat ccggctatcg cctcatcatt gcaggatcaa ctctcggctg
      601 tcgcagaagc ggaacagcaa ggtaaaaatg aagagcaaaa gcagcatgac gaatgggtcg
      661 cggagcgcaa cagggaaatc cagcaagaga agcaacgtcg cgcaaatgcc caggccgccg
      721 ctaacaaaag agcggcaacg gcagcagcaa ataagaaagc tcgtcaggat aaactggacg
      781 ccgaagcctc tgcggacaaa aaacgcgatc aaagttatga agatgagcta cgcagcttag
      841 agattcagaa acaaaaactg gcgctggcga aagaagaagc ccgcgttaag cgagaaaacg
      901 aatttatcga tcaggaactg aagcacaaag ctgcgcaaac cgatgtggtg caatctgaag
      961 ctgacgccaa cagaaatatg actgaaggcg gtcgcgatct gatgaaaagc gtgggcaaag
     1021 cagaagagaa caaatcggac agctggttta attaatcgat gttagtaact tcaagcctat
     1081 gattcttgaa gataaaaaac cctctgtagt aacagagggt tttgttcatt catagtgcag
     1141 ggatcaaaat cattcccact caatggtagc tggcggcttg ccgctgatgt catacaccac
     1201 gcgggaaata ccgttcactt cattgataat gcggttggaa acgcgaccga ggaaatcgta
     1261 cggcagatgc gcccagtgtg cggtcataaa gtcgatggtt tcgacagcac gcagagagac
     1321 aacccagtca tacttacgac catcgcccat tacgccaacg gaacgtaccg gcaggaacac
     1381 agtgaacgct tggctgactt tgtcgtacag gtccgcttta cgcagttctt caatgaagat
     1441 ggcgtcagca cggcgcagca ggtcacagta ctctttcttc acttcaccca gcacacgaac
     1501 gccaaggcct ggtcccggga acgggtgacg gtacagcatg tcgtacggca ggcccagctc
     1561 cagaccaatc ttacgcactt cgtctttgaa cagctctttc agcggttcaa ccaggcccat
     1621 cttcatctct ttcggcaggc cgcccacgtt gtggtgagat ttgatgacgt gtgctttacc
     1681 ggttgcagaa gccgcagatt cgataacgtc agggtagatg gtgccctgcg ccagccactt
     1741 cacgtcttcc agtttcagcg cttcttcatc gaatacttca acgaaaacgc gcccgatgat
     1801 tttacgtttt gcttccggat cgttttcgcc agccagcgct gacaggaagc gatcttctgc
     1861 cggtacgtga acaatgttaa gaccaaagtg atcgccaaac atatccagaa cctgctctgc
     1921 ttcgttgaga cgcagcaggc cgttgtcgac gaatacgcaa gtcaggtttt taccgatagc
     1981 gcggtgcagc agcattgcgg ttacggagga atccacaccg ccagagaggc cgaggatgac
     2041 tttatcgtcg cctacctgct cgcggatgcg agctacagca tcgtcgataa ttttcgctgg
     2101 cgtccacagg gcttcacact ggcagatatc acgcacaaaa cgctccagca tgcgcatacc
     2161 ctggcgggtg tgagtcactt ccgggtggaa ctgtacgcca tagaagcgtt tttcttcgtt
     2221 agccataatg gcaaacgggc agctttcggt gctggctacg gtgacgaagt cggacgggat
     2281 agcggtaact ttatcgccgt ggctcatcca gacatcgagc agcggtttac cgtctgcggt
     2341 cagcgcatct tcgataccgc gaaccagtgc gctgtcgttt acgacttcaa cctgcgcgta
     2401 gccaaattca cgttcgttag aggcttcaac gtgaccgccc aactgcattg ccatggtctg
     2461 catgccatag caaacgccga ataccggtac gcctgcttca aagacatact gcggcgcacg
     2521 cgggctgttt tcttcggtgg tgctttccgg gccgccggaa agaataatgc cgcttggatt
     2581 gaagtcacga atttgtgctt ctgtcacatc ccacgcccac agttcgcagt aaacacccag
     2641 ctcacgcacg cggcgcgcaa ccagttgagt gtactgagaa ccgaagtcca gaatgaggat
     2701 gcgatgctta tgaatgtttt ccgtcattga cgcttattcc gaggcaagtg aaacagataa
     2761 tataaatcgc ccgacatgaa gtcgggcgaa gagaatcagg agcccagacg gtagttcggg
     2821 gactctttag taatggtcac gtcgtgaacg tggctttcct gaatgcccgc accgctgata
     2881 cgtacaaact ccgctttagt acgcagttcg tcgatagtac cacagccggt cagacccata
     2941 caggagcgca ggccgcccat ctgctggtga atgatctctt tcaggcgacc tttataagct
     3001 acgcgacctt cgataccttc cggcaccagt ttgtcggcag cgttatcgct ctggaaataa
     3061 cggtcagagg aacctttgga catcgcgccc agggaaccca taccacggta agatttgtaa
     3121 gaacggccct ggtagagttc gatttcaccc ggagattctt cagtacccgc cagcatggaa
     3181 cctaccatca ccgcgcttgc gccagcggcg atagctttgg cgatgtcgcc ggagaagcga
     3241 ataccaccat ctgcgataac cggaataccg gtgccttcca gggcttcaac tgcgtcagca
     3301 acggcagtga tctgcggaac acctacgcca gtaacgatac gagtcgtaca gatagaacca
     3361 gggccgatac cgactttaac cgcgctgcaa ccagcttctg ccaaagcgcg tgcacctgca
     3421 gctgttgcca cgttgccgcc gatgatttgc agatccggat atttagcacg ggtttcacgg
     3481 atacgctgca aaacgccttc tgagtgaccg tgggaggagt cgatcagcag aacgtcaacg
     3541 ccagcggcaa ccagcgcgtc aacacgctct tcgttacccg cacctgcgcc aactgcagca
     3601 ccaacacgca gacggccttg ctcgtcttta caggcgttcg gtttacgttc cgctttctgg
     3661 aagtctttca cggtgatcat gccgatcagg tggaattcgt catcaaccac cagcgctttt
     3721 tcaacgcgtt tttcgtgcat ttttgccagc accacttcac gggcttcacc ttcacgcacg
     3781 gtgaccagac gctctttcgg cgtcatgtaa acgctaaccg gctggttcag gtcggtaaca
     3841 aaacgcacgt cacgaccggt gataataccc accagttcgt tttcttcggt aacgaccgga
     3901 tagcccgcaa aaccgttacg ctcagtcagt tctttcactt cgcgcagcgt cgtggttggc
     3961 agaacagtct gcggatcagt caccacacca gattcgtgtt ttttcacacg gcgaacttct
     4021 tctgcctggc gttcaatgga catgtttttg tggataaagc cgataccgcc ttcctgagcc
     4081 agagcaatag ccaggcgcgc ttccgttacg gtatccattg ctgcggaaag catagggata
     4141 ttcagacgaa tagttttcgt cagctgggtg ctgaggtcag cagtattcgg cagaacggta
     4201 gaatgagcag gaacgaggag aacgtcgtca aacgtcagag cttctttagc gatacgtagc
     4261 atgggcaata tctcgaccag agtggttaat aaatattgcc gcggcattat acagagcgta
     4321 accgattgca tctacccctt tttgcaaaaa atgcttgcta tccccgaagg gcgggttact
     4381 atcgactgaa taacctgctg atttagaatt tgatctcgct cacatgttac cttctcaatc
     4441 ccctgcaatt tttaccgtta gtcgcctgaa tcaaacggtt cgtctgctgc ttgagcatga
     4501 gatgggacag gtttggatca gcggcgaaat ctctaatttc acacaaccgg cttccggtca
     4561 ctggtacttt acactcaaag acgacaccgc ccaggtacgc tgcgcgatgt tccgcaacag
     4621 caaccgccgg gtgaccttcc gtccacagca tggacaacaa gttttagttc gcgccaatat
     4681 tacgctctac gagccgcgcg gtgactacca gataatcgtt gagagtatgc agccggccgg
     4741 tgaagggctg ctgcaacaga agtacgaaca gctcaaagcg aagttgcagg ctgaaggttt
     4801 gttcgatcag caatacaaaa aaccacttcc ctcccctgcg cattgcgttg gtgtgatcac
     4861 ctcaaaaacc ggtgctgcgc tacatgatat tttgcatgtg ttaaaacgtc gcgatccttc
     4921 tctaccggtg atcatctacc ccaccgccgt tcagggcgat gacgcaccgg ggcaaattgt
     4981 tcgcgccatt gaactggcga atcagcgcaa tgagtgcgac gtgttgatcg ttgggcgcgg
     5041 cggcggttcg ctggaagatt tatggagttt taacgacgaa cgcgtagcgc gggcgatttt
     5101 tgccagccgc attccggtcg tcagcgccgt cgggcatgag acggatgtga ccattgccga
     5161 ttttgttgcc gatctgcgtg cgccaacgcc gtctgccgcc gctgaagtag tgagccgtaa
     5221 tcagcaagag ttactgcgcc aggtgcaatc gacccgtcaa cggctggaga tggcgatgga
     5281 ttattatctc gccaaccgca cgcgtcgctt tacgcagatc catcaccgat tacagcaaca
     5341 gcatccgcag ctccggctgg cacgccagca aaccatgctt gagcgcctgc aaaagcggat
     5401 gagctttgcg ctggaaaatc aacttaagcg taccgggcaa cagcagcagc ggttaacaca
     5461 gcggctgaat cagcaaaatc cacagccgaa gattcatcgc gcgcaaacgc gcattcagca
     5521 actggaatat cgtttagcag aaaccctgcg cgtacagctt agcgccacgc gtgaacgttt
     5581 cggtaatgca gtaacgcacc tcgaagccgt aagcccactg tcaacgctgg cgcgtggata
     5641 cagcgttact actgctactg acggcaatgt actgaaaaaa gtgaagcaag ttaaagcggg
     5701 tgaaatgcta accacacgtc tggaagacgg ctggatagaa agtgaagtta aaaacatcca
     5761 gccagtaaaa aaatcgcgta aaaaggtgca ttaagccagc acgaattcaa cgcgtttttt
     5821 cgatatcaag ccatgcccgt gctgacagaa gtaatctact gcaccgcagg cttttaacac
     5881 ctgcaacggt tgatggcagt ctggacaaag agctttcatt tcaataattt ccccgcagga
     5941 tgggcaacgc gcatggccgt tatcctgatc aagcacatgc tgacattgtg gacaatgcag
     6001 ttccatatga attcctccgg tagccatccc tctacattca tagagggaat ggcagataaa
     6061 atacttacgg ataattattt atttttcttg atgtgcttca tcagacgctt acgtttacgc
     6121 atctgggttg gcgtcagggt gttacgcttg ttcgcatacg ggttttcccc ttctttgaac
     6181 tgaatacgaa tcggcgatcc cattacgtcc agcgatttgc ggaagtagtt catcaagtag
     6241 cgcttgtagg aatcaggcag gtctttcacc tgattaccgt gaatcaccac aatcggcggg
     6301 ttataaccac cagcgtgggc atatttcagc ttcacacgac gaccgcgtac cagcggcggt
     6361 tggtgatctt caacagccat cgtcatgatg cgcgtcagca tagaggtccc cacacgacgg
     6421 gtggagctgt catacgcttc acgtactgat tcaaacaagt taccaacacc actgccgtgc
     6481 aaggcagaga taaagtgcac acgagcaaaa tcgataaagc ccagacggaa gtccagcgtt
     6541 tctttcacct gctctttcac ttcctggctc aggccatccc acttattcac cacaatgaca
     6601 agtgagcgcc cactattgag aataaagccc agcagcgaga gatcctgatc ggaaatacct
     6661 tcgcgcgcat caatcactaa catcaccacg ttggcatctt caatggcctg caacgttttg
     6721 attacggaga atttctctac agcatcggtg attttgccgc gtttacgtac gccagcggtg
     6781 tcaatgagca catactcacg tccatcgcgt tccattggaa tgtagatact gtcacgcgtc
     6841 gtgccaggca tgtcataaac aacaacgcgc tcttcaccaa gaatacggtt agtgagtgta
     6901 gacttaccta cgttcggacg acccacaatc gccagtttga tcggcagact ttgcgggtcg
     6961 aagtcgtctt cctcttcttc ttcgccgttc tcttccgctt caaattgcgc ccagtattca
     7021 gcgtcttcgt cgacttcctc ttgcggtgcg agatcttcca tccacggcag cagcacatgc
     7081 tccagcagac ttaatacgcc acgaccgtga gacgcggcga tcgggtagat ttcacctaaa
     7141 ccaagcgagt agaaatcaac cactgcctga tcgggatcca gaccgtcagt tttgtttgcc
     7201 accaggaagg tcggtttttc acgggagcgc agatgtttgg caatcgcttc atctgccggc
     7261 atcaggcccg cgcgcgcatc caccataaac agtacgacgt ccgcttcttc aatcgccagc
     7321 agcgactgtt ccgccatgcg ggtttctaca ccgtcttctg tgccatcaat cccgccggta
     7381 tcaatacaga taaactcacg gccttcaatt tccgcacgac cgtacttacg gtcacgagtc
     7441 agacccggga aatccgcaac cagcgcatct cgggtgcgag ttagacggtt aaataacgtg
     7501 gattttccta cgttagggcg cccgacaagc gcgaccacag gtaccatgtt taaagcctca
     7561 tttttataaa tcatcagaca acgcacgcta tattcgcgtc gttgttaaaa acaggaaaac
     7621 ggcccctgtc caggagccgt tttcaaagtg aacgacagag acgattaacg tgtaatagag
     7681 tacacggttc cgtcttttgc ctggatcagc agtttgccgt cagcggcaac cggttcagtc
     7741 tggaaaccgg aactatcaac tttttgctgg gcaacgaaac gaccatcttc gacgttaatc
     7801 cagtgcagat aaccttcact gtcaccgacc accaggttgc cattatacag caccggagaa
     7861 gtcagcaggc gatgcagcag atcgctttgt gcccacagcg taacgccgcc atcaatggtc
     7921 aacgccatca cccggtcatt ttgatcgacc agatagatgc gattgccgtc gacgatgaaa
     7981 tcattcaccg aacccagttc gcgtttccac ataatctgac cactgcgcag atcaagcgcc
     8041 gtcaggttac cattataggc cagcgcgaaa acaacgccgt taacaacgac gggagtcgtg
     8101 tcaacatcgc tcagacggtc aatttcggta gaaccggtcg cctgggaaat acgctgctgc
     8161 caaatcatct ggccctgttc catcagcact gcgctgacgc gaccattatc gccccccacg
     8221 acggccgcac caaaagccgt tgccggcgca gactcgccac gcaaagagag cgaaggcata
     8281 tcgaggttaa ctgtccattt gacagcgccg tcagcttcgt tcagcgcttg taactgaccg
     8341 ttactggtgt ggattaacac cagaccgtcg ctgaccaccg ggcgcgaaag tgcttcaccc
     8401 gcgactttag tttgccatgc cacagtacca tcgctggtat tcagcgcgta aacctgcgcc
     8461 ttttcgctgc caatgtagac atgcccacca gacacggtca caccgccaga aagtaatgca
     8521 ggctctttag agaaccagcc atctttctcg gccaggctga cagaccagat ttctttgcca
     8581 tcatccgcat tcagcgcttt taccaaacca gcgcggtccg ctgcatagac aacgttgtcc
     8641 gccagtgccg gatgaagatt ggaatagaag ttgccaatgc cgctaccaac ggaagtactc
     8701 cacgccgtgg tcggcgtaaa ctggttttca acggttggca atggggacat ctttaccaca
     8761 tcttcttcgc tgttaaacag cgaacagccg cttaaaaggg taacggaaag cagtcctggc
     8821 agcagtaatt tacgcaattg catcgggtcc ctctcagatg gacaaattat taattttcat
     8881 ctgcatcatt tcgctcagtg ccggagtaac atcgcttttc acgcctgctt cccatgcact
     8941 acgcgcacct tgcttatcac ctttgctcag caatgcttca ccacgcaggt cggcaacaat
     9001 ggcagcccac ccttcacctt tgatggtatc aagggttttc agcgcggcat cagcctgctt
     9061 gagctgtacc tgaacgcgag caagacgcag atttatcacg gctttgagat tttcatcgct
     9121 cgtgtctgcc agcccctgtt gtaactgggc ggcagctttc tctagttcat ttttgtcaac
     9181 aaattgctgc gcaagttcca aagaagccag cgcaccataa gtatttttat tttcagcagc
     9241 aaatttttcc gccgccggga tgctatccgg tttgccttcg ctcactgcgg taaccgcatt
     9301 ttgataggca agagaagcgg agcgtgcaga atcaacctga tggctgttcc agtagcgcca
     9361 gccaatcagt gcgccaacgc ccaaaatcac cccaacagcc agtgctttgc cattttcagc
     9421 aaaaaagcgt ttaaccgctt ctacctggtc gttttcgttc tcgtaaattt ccacgctgtc
     9481 cttctccttc cttaacccag taacgtgcgc aaatgcgcgg ctacgctatc ctgcgcaact
     9541 gccgtttgct caccagagcg caaatccttc actactgctg tgccgttagc cacttcagac
     9601 tcacccagca ccacagcaac gcgggcaccc catttatcag cacgggcaaa ctgtttctta
     9661 aagttgccgc cgccgtggtt ggtcatcaat ttcacgcccg gtaattcatc acgcagacgc
     9721 tcagctaatg ccatagccgc agattgtgta tcagcacctg aagccaccag gtatatatcg
     9781 acaacaggat cggctttaaa ttccggatta acggcctgta ctaacaatac aagacgttcg
     9841 aggcccatcg caaaaccgac agccggtgtt gcacgaccgc ccagttgttc cacaagaccg
     9901 tcataacgac cgcctgcaca cacggtgccc tgggagccga gactgttagt cacccactcg
     9961 aaaacggtac ggttatagta atccagacca cgcaccagac gctggtttac ggtgtaagcg
    10021 atccccgcgc tctcaagcag tttgcacaga ccggcaaaat gctcacgaga ttcctcgtcc
    10081 agataatcac ctaatgccgg agcgtcgttg agaagcgcct gcacttccgg attttttgaa
    10141 tccagcacgc gcagcgggtt agtgtacatg cggcgtttgc agtcttcgtc cagcttttct
    10201 ttatgctgct caaggaatgc caccagcgca tcgcggtaat tggcgcgtgc ttccagcgaa
    10261 ccgatagaat tcagctcaag agttacgtgt tcggaaatac ccagcgcgcg ccaccagcgg
    10321 gcggtgagca taatcagttc agcgtcgata tccggacctt gcagaccgaa aacttcgcag
    10381 cccaactgat ggaactgacg ataacgccct ttctgcggac gctcgtgacg gaacatcggc
    10441 ccgatatacc acagacgctg ttcctgattg tacagaagac catgctcgat gccggcgcgt
    10501 acacagcccg ccgtcccttc agggcgcaga gtcaggctgt cgccattgcg atcctcaaag
    10561 gtgtacatct ctttttcaac cacgtcggtg acttcaccaa tcgcacgttt gaatagcggg
    10621 gtctgctcta caatcggcaa gcggatttca ctgtaaccgt agctgccgag cacgtttttc
    10681 agtgtgcctt caatgcgctg ccagatggcc gtttcgccag gcaggtaatc gttcatgccg
    10741 cgaatggctt gaatgttttt tgccacgttt attctctttc tgaatataaa aatgaaccct
    10801 caacgcttcc ctcaatgttt cgggagctat gcgggttcaa tcatacacgg gaagcgaggc
    10861 gcttcccatc acgttattat ttttcaacct gctgaacgtc aattcgacgc gcttcgtcca
    10921 gctgactggc tttcgcacga atgcgtgctt ccagctggtc aatcatatcg ttgttgtcca
    10981 gacggtcttt gcgcacgcca tcttcataga ggccgctttt cttgttgccg ccggtgacgc
    11041 cgagtgtaga aaccagcgcc tcacctgggc cattcaccac gcagccgata atcgaaacgt
    11101 ccatcggagt gatgatatct tccaggcgtt gctccagcgc gttaaccgta ccgataacat
    11161 caaattcctg acgcgaacag gtcgggcagg cgatgaagtt gatcccgcgc gaacggatac
    11221 gcagcgattt caaaatatcg aaaccgactt tgatctcttc gaccggatcg gccgccagtg
    11281 atacgcgcag cgtgtcgccg atgccttcag acagcagcag acctaaacca atggcggatt
    11341 ttactgcccc gctgcgtgca ccaccggctt cggtgatccc cagatgcagc ggctgatcga
    11401 tctgttttgc cagcaaacga taagactcaa cagcgaggaa gacgtcagac gctttcacgc
    11461 tgactttgaa ctgatcgaag ttcaggcgat cgagatgatc aacatggcgc atggcggatt
    11521 ccagcaacgc ctgcggcgtc ggttcgccat acttttcttg cagatctttt tccagcgatc
    11581 cggcgttaac gccaatacgg atcggaatgt ttttatcgcg cgcacagtca accaccatgc
    11641 gaatacgctc ttcattaccg atattgccag ggttaatacg cagacaatcg acgccgtatt
    11701 ccgctacttt cagtgcaatg cgatagtcga agtggatgtc agccaccagc ggcacgttaa
    11761 cctgctgttt gatgagtttg aacgcttctg ccgcgtccat cgtcggtacg gagacacgga
    11821 cgatatcagc gccaacgcgt tccagcgcct tgatttgatt gaccgttgct tcgacgtctg
    11881 tcgtacgcgt attggtcatg gactgtacgg cgatgggagc accatcgcca atcggcacat
    11941 tcccaacgta aatacgtgtt gattttctac gttgaattgg agcctggtta tgcatgaaaa
    12001 atctcccgcg ttacccgtct gttactgcgc cggtgattgt tcggcattga gggtcagacg
    12061 cgcaacctgg ttagttctga taaaacgact cagatcgaca ggtttccctt gatactggat
    12121 ctgtactgcg gctggcgcac caattttcag tttgtatggt gcctggccgg ttaagttcaa
    12181 attaccgtct ttacgctgca taccgctaaa caatttttta ccggtagcat cagtgacctc
    12241 cagccagcaa tcggcagtaa agttcatcac cagcgcattc ggatcagcca ccggcgtggt
    12301 cacgccagcc tgatcggttg gcaacggcgc agcaccatct ggcgttgttg ctgccgttgg
    12361 tgccggggtc gcggcggtat caacatttgc ctgcgaaggc gaaacaaccg cattctgttg
    12421 cggatcaaca gctggtgctg gcgcagttac ggcaggtgtt tgcgtgttgg ttgcggtagt
    12481 atccacagac gctggcggcg tggacgttgt agccgggtct gtagttgtcg acgtatttaa
    12541 cggaacactc tgcccctgct cgctattact gctcagttcc gccgaagatt gatcggccat
    12601 agtggtgatc tcttcctgct gagctttgtg gtcttgccac caccaggcac cgctcaggcc
    12661 gataaccaca aacaacacca gccaggtgaa ggtcatcagc cagccgtcac gttttttgcg
    12721 gcgtttaccg agggaaaaac tctgcatcgg cgcaactttc gcagcccgta gtggagcctg
    12781 cttttccagc cctggcagca gttcttcttc tggaatatgt accagacgcg cataagagcg
    12841 gatatatccg cgcaggaatg ttgaagcaag atcggcgggt gccttatctt cttcaatgtc
    12901 gcgtaccgtg gaaaccttca ggcaaagtcg ctcggcaacg gcctgctgac taagtcctag
    12961 ttgttcgcga gcattacgca ggcgagcgcc ggtagtaagt gcttcatttt ggtcgtgcgt
    13021 ggcttcagta ttcattcgct gcaggtacgt ttaaatgaga attgagatgc cggtgaacca
    13081 tcatgctcac ccacaccgcg aaacatccgt taagttaacc gttatcatac agtataagac
    13141 cgtctgtgtg ctcgtgacaa agcccacatt cactaaggct aacttactgt tgcatcgtta
    13201 catactgcct taaagtcagc aaaaacgcac cgttaatatt gaccagacaa ttgcaactta
    13261 atacttcatt cataaaagat tacgccacgg tacataaagt aaccgtggcg taatggctat
    13321 cagaccgctt taatgtcgat ggcttcaccc tgcatccgtt tacgcagggt acgtttcgta
    13381 cggtcgataa catcgcccgc caactgacca caggcagcat cgatatcatc accacgggtt
    13441 ttacgcacaa tagtggtgaa accgtagctc atcagcactt ttgagaaacg gtcgatacgg
    13501 ctgttcgagc tgcgtccata cggcgcgccc gggaaggggt tccacgggat caggttgatc
    13561 ttacacggcg tatctttcag cagttccgcc agttggtgcg cgtgttcagt gccgtcgtta
    13621 acgtggtcaa gcatcacata ctcaatagtg acacggccct gattggcgtt ggatttctcc
    13681 agataacggc gcaccgcagc aaggaacgtt tcgatattgt actttttgtt gatcggcaca
    13741 atttcgtcac gaatttcgtc gttcggcgcg tgcagggaaa ttgccagtgc aacgtcgatc
    13801 atatcgccca gtttatccag cgccggaact acaccggaag tagaaagcgt gacgcgacgt
    13861 ttagacaggc caaaaccgaa atcatcaagc atgatttcca tcgccggaac gacgttgttc
    13921 aggttgagca gcggctcgcc catgcccatc atcactacgt tagtgattgg gcgctgaccg
    13981 gtgacttttg ctgcgccgac gattttcgcc gcacgccaca cctggccaat aatttccgat
    14041 acccgcaggt tgcggttaaa gccctgctgg gcggtggaac agaatttaca ctccagcgca
    14101 caccccacct gcgaagagac gcagagcgtg gcacggtcgt cttccgggat atacaccgtt
    14161 tcgacgcgct gatcgccaac ggcgatcgcc catttaatgg tgccgtcgga tgaacgctgt
    14221 tcttcaacca cttccggtgc gcggatctcc gccacctctt tcagtttgcc gcgcaacact
    14281 ttgttgatgt cggtcatctc atcaaagttg tcgcagcaat agtgatacat ccacttcatc
    14341 acctgatcgg cgcggaaggg tttttcacct aaatctttaa aaaactcccg catctgctga
    14401 cggttgagat ccagcaggtt gatttttcca tctttcgtgg tgacgttttc aggtgtgact
    14461 aattgttcag acatatgcta ttccggcctc gttattacac gttatggccc ctggagggtt
    14521 gaaaaaagaa acgccccggt gagcggctgc tcgtccgggg gcgctgcatt gtacaaattc
    14581 tggcgcacgg atgccacgtt tgcacgcggc atttacgaaa ttattaacgg gtgcgcgggc
    14641 acacttcgcc ttcgccaaag aaataagcga tttcgcgagc ggcagattcg acggaatcag
    14701 aaccgtgggt accgttttcg gtcaggctgt cagcgtaatc agcgcgcaga gtaccagcca
    14761 gtgcgtttgc cggattggtc gcgcccagca gatcgcggtg acgctgaacg gcgttttcac
    14821 cttccagcac ggaaaccacg atcgggccag aggtcatgaa ttcaaccaga ccatcaaaga
    14881 acggttttcc atcgtgttca gcataaaagc cacgtgcctg ttcaacggtc aggtgcagca
    14941 ttttggtgcc gacaattttg aaccctgcag cttcaaagcg cgcaaagata ttaccaatga
    15001 cgttttttgc taccgcgttc ggtttgatga tggaaaaagt acgttcaata gccattttta
    15061 cctctgtaaa ttgttctgtt gttgtctgta ccagcgtacg agatggcgcg gattataatg
    15121 agcaacaggg ccgttgacta ttgatgaagg taacattttt ttaaaat
//
LOCUS       DAFNBJ010000113        15161 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000113 DAFNBJ010000000
VERSION     DAFNBJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.113"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            48..320
                     /locus_tag="L2X49_003395"
     CDS             48..320
                     /locus_tag="L2X49_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBN4798426.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(321..1193)
                     /gene="rluF"
                     /locus_tag="L2X49_003396"
     CDS             complement(321..1193)
                     /gene="rluF"
                     /locus_tag="L2X49_003396"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBN4798427.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1541..2488
                     /locus_tag="L2X49_003397"
     CDS             1541..2488
                     /locus_tag="L2X49_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBN4798428.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2558..3364
                     /locus_tag="L2X49_003398"
     CDS             2558..3364
                     /locus_tag="L2X49_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBN4798429.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSKRASYITGVTTNIAGGKTRG"
     gene            3374..3781
                     /locus_tag="L2X49_003399"
     CDS             3374..3781
                     /locus_tag="L2X49_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HBN4798430.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            3781..4275
                     /locus_tag="L2X49_003400"
     CDS             3781..4275
                     /locus_tag="L2X49_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBN4798431.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4341..5138
                     /locus_tag="L2X49_003401"
     CDS             4341..5138
                     /locus_tag="L2X49_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBN4798432.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            5149..5973
                     /locus_tag="L2X49_003402"
     CDS             5149..5973
                     /locus_tag="L2X49_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBN4798433.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHQVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6025..7254
                     /gene="sorE"
                     /locus_tag="L2X49_003403"
     CDS             6025..7254
                     /gene="sorE"
                     /locus_tag="L2X49_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HBN4798434.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            7637..8326
                     /gene="pepE"
                     /locus_tag="L2X49_003404"
     CDS             7637..8326
                     /gene="pepE"
                     /locus_tag="L2X49_003404"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBN4798435.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8417..10048)
                     /locus_tag="L2X49_003405"
     CDS             complement(8417..10048)
                     /locus_tag="L2X49_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBN4798436.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10268..13951)
                     /gene="metH"
                     /locus_tag="L2X49_003406"
     CDS             complement(10268..13951)
                     /gene="metH"
                     /locus_tag="L2X49_003406"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBN4798437.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14151..14975
                     /gene="iclR"
                     /locus_tag="L2X49_003407"
     CDS             14151..14975
                     /gene="iclR"
                     /locus_tag="L2X49_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBN4798438.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3597 a         3996 c         3978 g         3590 t
ORIGIN      
        1 aacgaattcc cgtacactga actctctggc tcactgaggg caccatcatg gcactccccc
       61 gtattaccca aaaagagatg actgaacgcg agcagcgtga acttaaaaca ctgctggatc
      121 gggcgcggat tgcgcatggt cgcgtactga ccaactccga aaccaacagc attaagaaag
      181 agtacatcga taaattaatg gtcgagcgtg aggctgaagc gaaaaaagcc cgccagttga
      241 agaaaaagca ggcttataaa ccggatcctg aagcgtcatt tagctggtca gcaaatacgt
      301 cgacgcgcgg caggcgctga ttagcgtccc ttctttttac gccccggcga ggtaaaacgc
      361 ttaccgttgg acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga
      421 cgtttgatgc ccgctgtttt cggtttagcc ttcgctttag gttttacctc ggaagaggaa
      481 ttttcaatga gtttaaacag gtcgattaac tcatcgtcag ttaaatcgcg ccactcaccc
      541 agcggaatgc cgcttaaact gacgttcata atgcgcgtac gttccagctt tttcacttca
      601 tagccgaaat gctcgcacat gcgccggatc tgacggttca gcccctgcac cagggtaatg
      661 cggaagacaa acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc
      721 ggcacccccg cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc
      781 agatactctt tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga
      841 ttggtgagga aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt
      901 ttgctgtggt taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca
      961 ataccgacgg gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc
     1021 aactgaccgt ttactttcac aatgtcgccg ggtttcacct gatcgcctat ggtggctcgc
     1081 ttgccattaa ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa
     1141 attccgcttt cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct
     1201 cctgtaaaac gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac
     1261 gtcgaaatta aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat
     1321 gcaaatttgc ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc
     1381 tgatgactac tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa
     1441 agagtgaaaa atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag
     1501 aaagcgtatt tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat
     1561 ccgtttgatt gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat
     1621 cgcgcgcgaa ctgcgtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga
     1681 tcagggaatt gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca
     1741 gcaactgaag caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga
     1801 tgaagagact caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct
     1861 ggagcctggc gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa
     1921 cttgccgcag gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc
     1981 cggtaaactc gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct
     2041 gaaaggggaa tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa
     2101 tgggatcatg cagtctcagc actttaaaac catctctgcc tactgggata atctggatgt
     2161 cgccctggtg ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta
     2221 tggtggtgaa gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg
     2281 cttttttgat attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat
     2341 cgaaatgaat aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa
     2401 atacagcgga attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag
     2461 cagcacagct gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt
     2521 acggcgggtc cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca
     2581 ggataaaatc attatcgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga
     2641 attattagca caaggcgcaa acgtacagat ggtagatatt cacggtggcg atggtcaata
     2701 tgaaggtcat aaaggctatc agttttggcc gaccgatatt tccagcgcta aagaggtaaa
     2761 tcatacggta gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc
     2821 cggggtcaat ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact
     2881 caacgaagct gcattcgaaa aaatggtcaa tatcaaccag aaaggcgttt ttctgatgtc
     2941 gcaggcggtg gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc
     3001 agaaagtgga ctggaaggct cagaaggcca aagctgttac gccgctacca aagccgcgct
     3061 taatagcttc acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg
     3121 tatcgcgccc gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct
     3181 ggcgtggacg cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat
     3241 tcctattggg cgcgccggaa gattagcaga agtcgctgat tttgtttgtt atctgttgtc
     3301 taaacgcgcc agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg
     3361 ctaaggaggc tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat
     3421 gctggaatcg gttcagatgg tttacggcaa cgccaatgtt gaagccgttg aatttgtgcc
     3481 cggcgagaac gccggagaca tcgtagcaaa gctggaaaag ttagtaagca ttcacaatca
     3541 agatgagtgg ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcgat
     3601 gcttgcgatg cgcaatcctc gtctgcgggt aatcagcggc ctttctctgc cgctggcgct
     3661 cgaactggtt gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca
     3721 aatcgcaaag caaacctgcg ttgtctggaa acaactggca acggcagagg aggatttctg
     3781 atgaacatta cgctcgcccg cattgatgac cgcctgatcc acggtcaggt cactaccgtc
     3841 tggtcgaaag tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat
     3901 gaagttcgcc ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc
     3961 aatattgaaa aagccgtcgc tgtttatcac aacccgcaat atcaggacga aaccgttttt
     4021 tatcttttca ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaatagac
     4081 accctgaata ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt
     4141 tcattagatg atgatgatat taacgcattt catgagttaa ataatcttgg cgtcatactg
     4201 gatttacgcg tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag
     4261 ttaatcgcaa attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga
     4321 cacttaaaag gtgaaaaatc atggaaataa gtaccctaca aatcattgct atatttcttt
     4381 tttcctgtat tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa
     4441 ttgcctgtac ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggtg
     4501 gtacgctgga attgatagct ctcggctgga tgaacgtcgg cgcggcgcaa tctccggatt
     4561 ctgcactcgc cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca
     4621 ccggaatcgc catcgcgttg cctgtggctg cggcaggcca ggtgctgact gtgtttgccc
     4681 gtaccatcac cgtagtgttc cagcacgcgg cggataaagc agcggaagaa gcacggtttc
     4741 gcactctcga tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gttgctattc
     4801 cggcactgat tgtctcactg ttcgtcagcg ccgatatggt gagcaatatg ctgagcgcga
     4861 taccggaatt cgtgacgcgc ggtctgcaaa ttgctggcgg ttttatcgtg gtggtcggtt
     4921 acgccatggt gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt
     4981 tcctcgcagg tggctacctc gatctcagtc tgctggcgtt cggtggcgtc ggcgtgatca
     5041 tggccctggt ctacatccag ctaaatccac agtggcgtaa agctgaacct caaccccagg
     5101 ccaccacctc caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga
     5161 aaaattacac gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc
     5221 tttaactttg aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag
     5281 cgactttacc cattaaaaga ggatcaggtt gcggcgctca ggcgacacca ggtgttcttc
     5341 aataccacgc cagccgtatg tggcccggtc atcggcgtca ctgccgccat ggaagaggcg
     5401 cgggccaacg gcgcggaaat tgatgacggt accattaacg gcatcaaagt cggtctgatg
     5461 ggaccattgg caggggttgg cgatccactg gtttggggaa cgctgcgccc gattaccgct
     5521 gcgctcggcg catctctggc actttctggc aacatcctcg gcccgctttt gtttttcttt
     5581 attttcaatg cggtgcgtct ggcgatgaag tggtatggcc tacagctcgg ctttcgcaaa
     5641 ggggtgaata tcgtcagcga tatgggcggg aatgtgctgc aaaaactcac cgaaggcgcg
     5701 tcgattctcg gactgtttgt gatgggcgtg ctggtcacca aatggacgtc aatcaacgta
     5761 ccgttggtgg tttcacaaac gcatgccgcc gatggctcca ccgtcaccat gaccgtgcag
     5821 aacattctcg accaactttg ccctggtttg ctggcgctcg gtctgacgct gcttatggtt
     5881 cgtctgctca acaaaaaaat taacccggta tggctgattt tcgccctgtt tggcttaggg
     5941 attatcggta atgcgctggg cttcctgtcc tgattcttcg ccccggcacg actgccgggg
     6001 ccatcgctca acatgaggtg gtttatgaaa acaacagctc tgcgtcttta tggtaaacgt
     6061 gatttacgcc tggaaacctt tgaccttcct gaaatgcagg aggatgaaat cctcgcgacg
     6121 gtggtcactg acagcctgtg cctctcttcc tggaaagagg ccaatctggg tgaaaaccat
     6181 aaaaaagtac ccgacgatgt ggcgaccaac cccatcatca tcggccacga gttttgcggc
     6241 gatattctgg ccgtgggtaa aaagtggcag cacaaattcc agccgggtca gcgttatgtg
     6301 attcaggcca acctgcaact ccccgaccgc ccggactgcc ccggctactc cttcccgtgg
     6361 gtgggcggcg aagccacgca tgtggttatt cccaacgagg tcatggaaca agattgcctg
     6421 ctggcatacg acggcgaaac ctattttgaa ggctcactgg ttgaaccgct ttcctgcgtg
     6481 attggcgcgt tcaacgccaa ctatcatctt caggaaggta gttataacca cacgatgggg
     6541 attcgcccgc aagggcgcat gctgatcctc ggcggcaccg gaccaatggg actgttggcg
     6601 attgattatg cgctacatgg acccgttaac ccgtcgctgc tcgtcattac cgataccgac
     6661 aacgataaat tgagttatgc gcgcaagcac tatccatcag aaccgcaaac actgattcat
     6721 tatctcaatg ccgccgatgc agcatttgat acgctaatgg cgctgagtgg cggtcacggc
     6781 ttcgatgata ttttcgtctt tgtgcctaat gaaggactgg tgactctcgc ctcttccttg
     6841 ctggcgacag atggttgcct gaatttcttc gccggaccgc aggataaaca tttcagcgcg
     6901 ccaattaatt tctacgatgt gcattatgca tttacccact acgtgggcac gtcaggcggc
     6961 aataccgacg acatgcgcgc ggcggtcaaa ctgattgaag agaaaaaagt gcaggccgca
     7021 aaagtggtaa cacatattct tgggctgaat gccgcgggcg aaaccacgct tgaattgcct
     7081 gccgtcggcg gcggcaaaaa gctggtgtat accgggaaat acctgccgct gacgtcactc
     7141 acgcagattc aggatcaagc actggcggcg attctggcgc gtcatcaggg gatctggtcc
     7201 ggtgaggcgg agcaatatct gctcactcat gcagaggcaa tttcccatga ttaatcgcgc
     7261 tacttaactc tgcatgtcgc tggctggtcg ccgcggcaac ttcgcgacct gatgtgcggc
     7321 tgattgctga agcggcccaa tgtgcacgag aaggataaaa aaacatcagc gccttaaggg
     7381 gcgctcaaat tggtggcaaa gtacacattg tttatgccgg atgcggaata aagccttatt
     7441 cggcctacat aatctcgaaa attcaatata ttgcagttat ctggtaggcc tggtaagcgt
     7501 agcgcatcag gcaatgctgt gtttgtcatc agtctcagcg cccggtgcaa tacgtgacat
     7561 cgtgagccat gtcagtaagt ggttaaattc gcagtggtag catgcctccc gtgatttctt
     7621 actaactgga gaagtaatgg aactgctttt attgagtaac tcgacgctgc cgggtaaagc
     7681 ctggctggaa catgcgctgc cgctgattgc tgaacagttg cagggtcgcc gttcagcggt
     7741 atttattcct ttcgctggcg taacgcagac ctgggatgat tacacagcga aaacggctgc
     7801 ggttctcgct ccgctgggtg tatctgtaac cggtattcat agcgttgtcg atcccgttgc
     7861 cgcgattgaa aatgctgaga tcgtgattgt cgggggcggg aatactttcc agttgctgaa
     7921 acagtgccgc gagcgcgggc tgctggcacc aattactgac gtggttaaac gtggtgcgct
     7981 gtatattggc tggagcgcag gcgctaacct tgcttgccca accattcgta ccaccaacga
     8041 tatgccgatt gtcgatccgc aaggtttcga tgcgctaaat ctgttcccgc tgcaaatcaa
     8101 cccgcacttc accaacgcgc tgccggaagg ccataaaggt gaaacccgtg agcagcgtat
     8161 tcgcgaactg ctggtcgtcg cgccagaact gacgattatt ggtctaccgg aaggtaactg
     8221 gatcacagtg agtaaaggtc acgctacgct gggtggcccg aacaccactt atgtgtttaa
     8281 ggctggtgaa gaagcggtgc cgctagaagc tggtcaccgt ttttaagtaa attgccggat
     8341 gcatgatccg gcatcttgtt catagtgcct gatgcgatgc ttgacgcgtc ttatcaggcc
     8401 tacaggattc acaatattaa tactcgtccc gtccttcatc ttcatccggc tgttccagca
     8461 cactgtaagc caccgaacaa aacagcgagt tcaggcgctg catatctccc agtaagccta
     8521 aatgtagcga actggtttca atgctctgca cgttttgctg atgcaggcga tcgacgtggg
     8581 cgtgggaata gcggcgatta agaatgcgaa aacgatgttt gctgcgacgc aaacgacgag
     8641 cgctggtgac atcgccagag aagaacaccg acattgccag ctttaaatta ctgagcaatt
     8701 gctcatagag cgcatccagt tccttcaacc catcaagcga aaatgcccgc cgtgctgcca
     8761 gcgatttatc ggcaatttcg ctgcccatgc gctcgacgat atcggaagcc tgttcaaggt
     8821 tgagcgacat ttcaataatc tccgcccagc gacgcgactc ttcttctgcc agttcctctt
     8881 ttggcatccg cgccagatac agcttaatgg cggtatagag aacgttgata tcatctgcca
     8941 gcttacgcag ctctttctcc tgccgtggct cgccgtgcat cactttattc agcccttcca
     9001 tcatctgttc catggcgtcg ccaatgcgca gggtttcgcg cgcggcgttc gccagagcaa
     9061 gcgtgggcgt atccagcgcg ctgacatcca gatgtttagg ccgtagctgg gtatccagtt
     9121 ccggttcatc gcgaataatc gttttgcaaa accgtgccat cgggtcaaca aatggcagca
     9181 tgaccaggca acgtacaagg ttgtagaaga cgtggaaata gatcaccagt tccgcttttg
     9241 gcaatgacag cttccccatt gtctctgcca gcaaatggac aaacggcagg ataatcaggc
     9301 tacccaccag cttaaacagc agactaccca gcgcgacacg gcgggctgcg gcattggcgg
     9361 cactgttgtt gagcatcgcc agcaggccgg aaccgaggtt agcaccaatc accagacaga
     9421 gcgccacggg gaaggagata atgcctgcgg cggtcagagt cgcagtcagc agtacagcag
     9481 caaggctgga gtagctgata atggcgaaca tcgcgccaat cagcgcatcc agcagaatat
     9541 cgccggtcag cgaggcaaag atcacctgaa cgccgtttgc ctgggtgatc ggcgttacgg
     9601 cctgcacaat caactccagc gccagtaaaa tcagcccaag accaataccg acgcggccca
     9661 gttgcccggc gcgtgactgt ttgcgtccga ggaaaaaaat cacgccgata aaaataagta
     9721 acggtgacag ccaggataag tcgaaggtga gaatacgcgc cattagcgcc gtcccgacat
     9781 ctgcccccag cacaatgacc agagccggtg cgagggctac cagatcctga gcgacaaacg
     9841 aggtcaccag catggtggtg gcattactgc tctgcaccag tgcggtaacg ccgatccccg
     9901 cgcaaaaggc gagcggcttt ttttcgacgc tccggctaag gacagtacgc aaacgcgcgc
     9961 cgaagacgcg cattacgccg gttcgaacaa tatgagtccc ccagaccagc agggcgacgg
    10021 cagaaagcag gtgaagcagc gttaacacga gttcaggttc tccttatcgt tatatgttcc
    10081 tgaagcaatt gaatgaccaa aggaccggaa acacgctgac tggcatcttt actgtgcctt
    10141 ttgacattcg tttttccggc taagattatt ccagcctgtt tccagtataa gtgtttcata
    10201 ttaataaaga gacagggcac tcattgagcg ccctgtttgt tgtaaggaaa agtgacagat
    10261 ttgtgaatca gtccgcgtca taccccagat tcggtgccag ccagcgctca acatcggaga
    10321 cgctcatacc tttacggcgg gcatagtctt caacctgatc gcgctgaatt tgcgccaccg
    10381 cgtagtactt gctgtccggg tggctgaaat accaacccga aaccgatgca ccgggccaca
    10441 tggcgaaaga ttctgtgagt ttcatgccgg tatgtttttc cacttccagc agctcccaga
    10501 tggtggcttt ttccgtatgt tccgggcagg ccgggtagcc cggtgccgga cggatgccct
    10561 ggtagttttc gcggatcagt tcttcgttgc tgaggttctc gttcggcgca tagccccagt
    10621 agactttacg cacacgctca tggagatact ccgcaaaggc ttcggctaaa cggtcggcaa
    10681 gcgctttcac catgattttg ttgtaatcat cgtgctgcgc ttcaaaggca tcagccagtg
    10741 cgtcctcttc cagcccgcca gtcacggcaa atgcgccgat gtaatctgct ttaccagaaa
    10801 gcttcggcgc aacgaagtca gcgagacagt agttagcgaa tcccgttttt tcggtctgtt
    10861 gacgcagatg gtggctgacg ttgatcacat gggtgcgcgt ttcgtcacgg tagatttcaa
    10921 tgtcatcgcc cacacggttt gccgggaaca ggcccaccac gccacgcgga ttcagcgttt
    10981 tctcggcgct taatttatcc agcatgtcgt tggcgtcttt aaacagccgc tgcgcctcaa
    11041 cgcccaccac ttcatcttcc agaatgcgcg gatacttccc ggccagcgac caggtcataa
    11101 agaacggtgt ccagtcgatg taattacgca gagtttcgat gctggcttca acttcctgca
    11161 cgccgagacg gtgcgccacc ggcggcgtgt aagcctgcca gtcaaaagcg aaatcgttat
    11221 cgcgcgccgc ttccagcgtg accggtggtg tgcgcggttt cttacgcccg tgctgaatac
    11281 gtacggtttc gtactccttg cgggtacgag cgacaaaatc atcacgctga gtatcggaaa
    11341 gcagcgccgc caccacacca acggtgcgcg atgcgttctg tacatacacc gtcgggccgc
    11401 tgtagttctg ctcaattttc accgccgtgt gcgcttttga ggtcgtcgcg ccgccaatca
    11461 atagcgggat ggtgaagccc tgacgctcca tctctttcgc cacgttaacc atctcgtcca
    11521 gcgacggcgt gataagcccc gaaagtccaa tcagatcagc attcacttct ttagcggtac
    11581 ggagaatttt ttccgcaggc accataacgc cgagatcgac aatttcgtag ttgttacatt
    11641 gcagcaccac accaacgata tttttaccga tgtcgtggac gtcgcccttc acggtggcga
    11701 tcaccatctt gccgttggtt ttgccctgct ctttgctggc ttcaataaac ggttcgagat
    11761 acgccaccgc ctgtttcatg acgcgcgccg atttgaccac ctgtggcagg aacattttcc
    11821 cttcgccaaa cagatcgccg accacgttca tgccgtccat caacgggcct tcaatcactt
    11881 caatcgggcg cgtagcctgc tggcgggctt cttcggtatc ctgctcgata aactcggtaa
    11941 tgcctttgac cagcgagtat tccagacgtt tattcacttc ccacgagcgc cactccgcct
    12001 gctgggcgtt agcggtgtcg tcggttttgc tgccgcgata tttctcggca agctccagta
    12061 aacgctcagt gccatcatcg cgacgattaa gaatcacatc ttccaccgcg tcgcgcagtt
    12121 cagcgggcag gtcgtcgtaa atcgccagct gcccggcatt gacgatcccc atatccatgc
    12181 cattgcgaat agcgtagtag aggaacactg cgtgaatggc ttcgcgcacc ggatcgttgc
    12241 cacggaacga gaaagaaacg ttagatacgc cgccggaaat tagcgcgtgc ggcagttcgc
    12301 gtttgatgtc ttcacacgcg ccgataaagt cctgcgcgta gttgttgtgc tcttcaatgc
    12361 cggtggcaac cgcgaagatg ttcgggtcga agatgatatc ttctggcggg aagccaacct
    12421 cttcggtgag gattttgtac gcccgacggc aaatctcgat tttccgtgcg cgggtatcgg
    12481 cctgtccctg ttcgtcaaag gccattacca ccactgccgc accgtaacga cgcaagagct
    12541 tcgcgtgatg gataaaggcg tcgacgccct ctttcatcga gatagagtta acaatgcctt
    12601 tgccctggat acacttcaga cctttttcaa tgacatccca ttttgaggag tcgatcataa
    12661 tcggcacgcg agcgatatcc ggttcaccgg caatcagatt gagaaaacgc accatcgccg
    12721 cttcggcgtc gagcatccct tcatccatgt tgatatcgat aatctgcgca ccgttttcca
    12781 cctgctggcg ggccacatcc agtgcctcgc tgtatttctc ttctttgatc aggcgcttga
    12841 acttagcgga accggtgacg ttggtgcgtt cacccacgtt cacaaacagg ctatcttcgc
    12901 caatgttcag cggctccagg ccggacaaac ggcaggctac ggggatttcc ggcagtttgc
    12961 gcggcgctaa tccttctact gcacgactca tcgctgcaat atgttgtggc gtggtgccac
    13021 agcagccgcc gacgatattg agaaaacccg cttgcgccca ttcacgtatc tgttttgcca
    13081 tcgtgtcggc gtcgagatcg tactcaccaa aggcgttggg tagcccggcg ttcgggtgcg
    13141 cggtgacgta gcattccgca atccgtgaca gctcctgcac gtactggcgc agttcatcgg
    13201 gccccagcgc acagttcagg ccaaaggtca gagcttcggc gtggcgcaat gagttgtaaa
    13261 atgcttcggt ggtctgcccg gagagcgtgc gcccggaggc gtcggtgatg gtgccggaga
    13321 tcataatcgg cagctcaacg cccagcgctt caaactccgt tttcaccgca aaaaccgccg
    13381 ctttggcgtt aagggtgtcg aaaacggttt caatcaggat cagatccgca ccaccttcca
    13441 ccagcgcttt ggtggattcg cgataggccg ccaccagctg gtcaaaagtg atgttgcgga
    13501 atgccggatc gttgacgtcc ggagaaatag acgccgtgcg gttggttggg ccgagaacac
    13561 cggcaacgta gcgcggtttc tccggcgtgc gcgcggtcca ctcgtcagca caggcgcgcg
    13621 ccagtttcgc cgccgcaaag ttgatttccg ccgacaggga ttccatctgg taatccgcca
    13681 tcgcaatggt cgtggagttg aaggtgttgg tttcgatgat atccgcgccc gcttcaaagt
    13741 aggcgttgtg gatagcggcg atcacttccg gtttgctgag caccagcagg tcgttgttgc
    13801 ctttgagatc gcatggccag tcggcaaagc gttcaccacg aaaatcggct tcgttcagtc
    13861 gatagctctg gatcatggtg cccataccgc cgtccagcac cagaatacgt tcatttaact
    13921 gcgcacgcag ttgttccact ttgctgctca cacttgctcc cgacacgctc aacccagatt
    13981 taataaaaat tcaacaaacc atactggcat aaacgcatct gtggtaaaag cgaccaccac
    14041 gcaacatgag atttgttcaa cattaactca tcggatcagt tcagcaacta ttgcattagc
    14101 taacaataaa aatgaaaatg atttccacga tacagaaaaa ggaggctgtc atggtcgcac
    14161 ccattcccgc gaaacgcggc agaaaacccg ccgttgccac cgcaccagcg actggacagg
    14221 ttcagtcttt aacgcgtggc ctgaaattac tggagtggat tgccgaatcc aatggcagtg
    14281 tggcactcac agagctggcg caacaagccg ggttacccaa ttccacgacc caccgcctgc
    14341 taaccacgat gcaacagcag ggtttcgtgc gtcaggtcgg cgaactggga cattgggcaa
    14401 tcggcgcaca tgcctttatg gtcggcagca gctttctcca gagccgtaat ttgttagcga
    14461 ttgttcaccc tatcctgcgc aatttaatgg aagagtctgg cgaaacggtc aatatggcgg
    14521 tgcttgatca aagcgatcac gaagcgatta ttatcgacca ggtacagtgt acgcatctga
    14581 tgcgaatgtc cgcgcctatc ggcggtaaat tgccgatgca cgcttccggt gcgggtaaag
    14641 cctttttagc ccaactgagc gaagaacagg tgacgaagct gctgcaccgc aaagggttac
    14701 atgcctatac ccacgcaacg ctggtgtctc ctgtgcattt aaaagaagat ctcgcccaaa
    14761 cgcgcaaacg gggttattca tttgacgatg aggaacatgc actggggcta cgttgccttg
    14821 cagcgtgtat tttcgatgag caccgcgaac cgtttgccgc aatttcaatt tccggaccga
    14881 tttcacgtat taccgatgac cgcgtgaccg agtttggcgc gatggtgatt aaagcggcga
    14941 aggaagtgac gctggcgtac ggtggaatgc gctgactttt tctggcgggc agaggcaata
    15001 ttctgcccat catacctgag tggcaataga ataagggtgt ctgttaatcg cattgacgcc
    15061 aaaataactt aatgtcatac acttcacaca cttttattaa ctcagcatta tttttaaaca
    15121 tcaaactact taatcattaa caaaatcgca agatctcatt c
//
LOCUS       DAFNBJ010000114        15012 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000114 DAFNBJ010000000
VERSION     DAFNBJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.114"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            146..688
                     /locus_tag="L2X49_003408"
     CDS             146..688
                     /locus_tag="L2X49_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798439.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            788..1990
                     /locus_tag="L2X49_003409"
     CDS             788..1990
                     /locus_tag="L2X49_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBN4798440.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALTTPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2210..2992
                     /locus_tag="L2X49_003410"
     CDS             2210..2992
                     /locus_tag="L2X49_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBN4798441.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGLPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3007..4212
                     /gene="rhmD"
                     /locus_tag="L2X49_003411"
     CDS             3007..4212
                     /gene="rhmD"
                     /locus_tag="L2X49_003411"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HBN4798442.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLMVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSLDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4269..5558
                     /locus_tag="L2X49_003412"
     CDS             4269..5558
                     /locus_tag="L2X49_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4798443.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5576..6379
                     /gene="yfaU"
                     /locus_tag="L2X49_003413"
     CDS             5576..6379
                     /gene="yfaU"
                     /locus_tag="L2X49_003413"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HBN4798444.1"
                     /translation="MNALLSNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     EQARQVVTATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6420..6617)
                     /locus_tag="L2X49_003414"
                     /pseudo
     CDS             complement(6420..6617)
                     /locus_tag="L2X49_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6618..7517)
                     /locus_tag="L2X49_003415"
     CDS             complement(6618..7517)
                     /locus_tag="L2X49_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBN4798445.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAKRSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLATTQLTDADIPNCH"
     gene            complement(7710..8900)
                     /gene="glpC"
                     /locus_tag="L2X49_003416"
     CDS             complement(7710..8900)
                     /gene="glpC"
                     /locus_tag="L2X49_003416"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBN4798446.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTAKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8897..10156)
                     /gene="glpB"
                     /locus_tag="L2X49_003417"
     CDS             complement(8897..10156)
                     /gene="glpB"
                     /locus_tag="L2X49_003417"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBN4798447.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10146..11774)
                     /gene="glpA"
                     /locus_tag="L2X49_003418"
     CDS             complement(10146..11774)
                     /gene="glpA"
                     /locus_tag="L2X49_003418"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBN4798448.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQDP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12047..13405
                     /gene="glpT"
                     /locus_tag="L2X49_003419"
     CDS             12047..13405
                     /gene="glpT"
                     /locus_tag="L2X49_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBN4798449.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13410..14486
                     /gene="glpQ"
                     /locus_tag="L2X49_003420"
     CDS             13410..14486
                     /gene="glpQ"
                     /locus_tag="L2X49_003420"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBN4798450.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDVL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14705..>15012)
                     /locus_tag="L2X49_003421"
     CDS             complement(14705..>15012)
                     /locus_tag="L2X49_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798451.1"
                     /translation="KLGGELIDIADKKLAPLINDSFSYTRDFFAYSKQENNIFTFDNS
                     KFVDPKEKEGLMIQHSNGQLVITGKYCPEGVQTAFTQEQYDKLIRYINIFFTFPKCE"
BASE COUNT         3469 a         3672 c         4068 g         3803 t
ORIGIN      
        1 attttttcgg cgtttaatta tttaagaagt atttagttac aataagtgac tagatccctt
       61 ttttcgctca ttgaatcctc tgtgccaggc agtacaatcc cgcagctttg ggaattttga
      121 ttaattgctt gtgggaaagg ggataatgaa aaaaattgcg ctagcaggtc tggccggaat
      181 gctgttggtt tctgcatcgg tcaatgcaat gagcatcagc ggccaggcgg gtaaagaata
      241 caccaatatt ggtgtcggtt ttggaactga atcgacgggc ctggctttaa gcggtaactg
      301 gacacataac gacgacgacg gtgacgtcgc gggcgtgggg ctggggttga atctgcctct
      361 cgggccgtta atggcgaccg ttggcggaaa aggcgtttac accaacccga attacggcga
      421 tgaaggttat gccgcagcgg taggaggtgg tttgcagtgg aaaattggca acagcttccg
      481 tttgtttggc gagtattact actctccgga ttcgctctcc agcggtattc aaagttatga
      541 agaagcgaat gctggcgcgc gttacaccat tatgcgtcca gtcagtattg aggcgggtta
      601 tcgctacctg aatctgtcgg gtaaagacgg taaccgcgac aacgccgtgg ctgacggccc
      661 gtatgttggg gttaacgcca gtttctgatc ccttccggcg cggcattctg tcgcgccatt
      721 cagtttctta ctacgcctta tctccctttg cgttatagtg attcgataac aaaagcagga
      781 gataaccatg ttaaaagtgg aaatgttatc caccggggat gaagtgttac acgggcaaat
      841 cgttgacact aacgctgcct ggctggccga ttttttcttt catcaggggt tgccattatc
      901 tcgccgcaat acggtggggg ataaccttga tgacttagtc accattcttc gcgaacgtag
      961 tcagcacgcc gatgtgctga tcgttaacgg cgggctggga ccgaccagcg atgatttaag
     1021 cgcactcgcc gctgcgacag caaaaggtga aggcctggtg ctgcatgaag cctggctcaa
     1081 agagatggaa cgctatttcc acgaacgtgg acgagtaatg gcaccgagca accgtaaaca
     1141 agcggagctg cctgccagtg ctgaatttat caataacccg gtaggcaccg cctgtggttt
     1201 tgccgtacag cttaatcgtt gcctgatgtt ctttactccc ggcgtaccgt cagaatttaa
     1261 ggtgatggtc gagcacgaaa tcctgccgcg tctgcgcgag cgtttttctt taccacagcc
     1321 gccggtttgt ctgcgtttaa ctacctttgg tcgttcggaa agcgatctgg cacaaagcct
     1381 ggacactcta caactgccgc cgggggtaac aatgggctat cgctcctcaa tgccgatcat
     1441 cgaactgaaa ctcaccggac cggcaagcga gcaacaggcg atggaaaaac tgtggctgga
     1501 cgttaaacgt gttgccggac agagcgtgat tttcgaaggc accgaaggac tgcccgcgca
     1561 gatcagtcgc gaattgcaaa accgccagtt cagcctgacg ttgagcgagc aattcaccgg
     1621 tggtttattg gctttgcaac tttctcgcgc aggtgctcca ttgctggcgt gtgaagtggt
     1681 tccttcacag gaagaaaccc tggcgcaaac tgcgcactgg attacagaac ggcgggccaa
     1741 ccattttgcc gggctggcac tggctgtttc gggtttcgag aacgagcatc tcaactttgc
     1801 gctaaccacg ccagacggca ctttcgctct gcgtgtgcgt ttcagcacta cacgctacag
     1861 cctggctatc cgtcaggaag tgtgcgcaat gatggcactg aatatgctgc gccgttggtt
     1921 aaacggccag gatatcgcca gtgagcatgg ctggattgag gttgttgagt ccatgacctt
     1981 atccgtctga ttccgccact ctgtgccggt ttactgcacc ggcacattcc ccctccggca
     2041 aaatttgtcc aacctgttgt actcaataag aaaaaatccg tgcgctgatt cacaaatttg
     2101 tgcgtaattt ctccagtccg ctggacgagg agcgggattt gatgcgcttc acaattttac
     2161 tccccctgag caacgagact gattccagta tctaagactg gagtccagta tgttggaatc
     2221 aagcaaagtt cccgccctga cacgggctat tgatattctc aatttgattg cccgcattgg
     2281 cccctgtaat gctgcgacga tcattgacac attggggctc cctaaaagca cggcctattt
     2341 gctgcttaat gaactcaggc gtcagcgttt tcttagcctt gatcaccagg aaaatttttg
     2401 tttgtggacc aggctggtgg agctttccgg tcatgcgtta agcaagatgg atctccggga
     2461 actggctcga ccgcgtttga cgcaactgat ggataccacc gggctattat gtcacctggg
     2521 gattattgat aacggcagcg cttattacat tttgaaagtg gagtcatcag ccactatcag
     2581 cgtgcgttca catgaaggaa aaagcctttc gctttatcgc tccggaatag gtaaatgcct
     2641 gcttgcctgg caacctgcag cggtacagca aagtattatc gaagggctgg tctgggagca
     2701 ggcgacaccc acaacgatta ctcatccaca gcaacttcac gaagaactgg cgcgtattcg
     2761 ccgccagggc tggagctatg acaacggcga agattacgcc gatgtccgtt gcgtcgctgc
     2821 gccggtgttt aacgccaata acgagcttac cgctgcgatc tcggtggtcg gtacccgctt
     2881 acaaatcaat gaagaatacc gtgattatct ggcgggcaag gccattgcct gcgccaggga
     2941 tatttcacgt ctgttgggat ggaaaagtcc ctttgactta caagcctcat aacaatggag
     3001 aacatcatga ccctaccaaa aattaaacag gttcgcgcct ggtttactgg cggtgcgaca
     3061 gcagaaaaag gcgctggcgg cggtgattat cacgaccagg gggcgaatca ctggatagac
     3121 gatcatattg ccaccccgat gagtaaatac cgcgattacg agcagtcacg ccagtcattt
     3181 ggcattaacg ttcttggcac gttgattgtt gaagtcgaag cagaaaacgg ccagaccgga
     3241 ttcgccgttt cgacagccgg tgaaatgggc tgttttatcg tcgaaaaaca ccttaaccgt
     3301 ttcattgagg gtaaatgtgt cagtgatatc aaactgatcc acgatcaaat gctcaatgcc
     3361 accctgtatt actccggctc tggtggcctg gtgatgaata cgatttcctg tgtcgatctg
     3421 gctctgtggg atctgttcgg caaagtggtc gggcttccgg tttataaact tttaggcggc
     3481 gctgttcgtg atgagattca gttctacgcc acaggtgcgc gtccggatct ggcaaaagag
     3541 atgggcttta tcggtggcaa aatgccgacg cactgggggc cacatgatgg cgatgcgggg
     3601 atccgcaaag atgccgctat ggtcgcggat atgcgtgaaa aatgcggtga ggatttctgg
     3661 ttaatgctcg actgctggat gagtcaggac gtgaactatg ccaccaaact ggcccacgct
     3721 tgcgcgccct ataacctgaa atggatcgaa gagtgcctgc cgccacagca gtatgaaggt
     3781 tatcgcgaac tgaaacgcaa cgcgccagtc ggaatgatgg tcaccagcgg tgagcaccac
     3841 ggcacactgc aatcttttcg tacgctttca gaaaccggta tcgacattat gcagccggat
     3901 gttggctggt gcggtggctt aaccacgctg gtggaaattg ccgcaatcgc caaatccagg
     3961 gggcaactga tggtgccgca cggttcgtct gtttactccc accatgcggt gatcaccttc
     4021 accaatacgc cattcagcga attcctgatg accagtctgg attgttcaac gatgcgtccg
     4081 cagtttgatc cgattctgct caatgagccg gttccggtga atggtcgtat tcataaatca
     4141 gtgcttgata aacccggttt tggcgtcgaa ctcaatcgtg actgcaattt gaaacgcccc
     4201 tacagccact aatcactccg ggcgctgctg tgacaacggt aacgcccaca tattctaagg
     4261 agctttttat gagcaccgct ttgcttgacg ccgtggtgaa gaaaaaccgt gtgcgtttaa
     4321 ttccgtttat gttggcgctg tatgtgctgg cgtttctcga ccgttcgaat atcggttttg
     4381 ccaaacagac ctaccagatt gataccgggt tgagtaatga agcttatgcg ctgggagcag
     4441 gcattttctt tgtggtatat gcgtttctgg gtgttccggc gaatcttttg atgcgcaaac
     4501 tgggggccag aacctggatt ggtacgacaa cactgctgtg gggatttctt tcggcagcca
     4561 tggcatgggc cgatactgaa gcgaaatttc tgatcgttcg cactctgctt ggtgctgcgg
     4621 aggctgggtt tttccctggt atgatttatc tcacctcgca atggtttccg cagcgtaatc
     4681 gcgccagcat tatggggctg ttctatatgg gcgcaccgct ggcgttaaca ctgggatcac
     4741 cgctttccgg cgcgctgctg gagatgcatg gatttatggg gcatcccggc tggttctgga
     4801 tgtttgtgat tgaaggattg ttggcagtcg gtgctggggt attcacattc ttttggcttg
     4861 atgacacacc ggagcaggca cgttttctga gtaaacaaga aaaaacgttg cttatcaatc
     4921 aactggcaag tgaagaacaa cagaaagtga cttcgcgact gagcgatgcg ttgcgtaatg
     4981 ggcgagtctg gcaactggcg attatctacc tgaccattca ggtggcggtt tacggattaa
     5041 ttttcttcct gccgacccag gttgcggcat tgctggggac aaaagtgggc tttacagcgt
     5101 cggtggtcac cgccattccg tgggttgctg ccttgtttgg gacctggctt attccgcgct
     5161 attccgataa aaccggcgaa cggcgtaatg tcgcagcgct gacattactg gcggcgggca
     5221 ttggtattgg tctgtccggg ctgctttctc cagtaatggc gatcgtagcg ctgtgtgttg
     5281 cagccatcgg gtttattgcc gtgcagccgg tgttctggac gatgccgaca caactgcttt
     5341 ccggtacggc gctggctgcg gggattggtt ttgtaaacct gtttggtgca gtgggcgggt
     5401 ttattgcccc gatcctgcgc gtgaaagcag aaacgttatt tgccagcgat gcggcgggat
     5461 tactgacgct ggcagcggtg gcggtcatcg gttcgctgat tattttcact ctgcgtgtaa
     5521 atcgcaccgt tgcgcagacc gacgtggcac atcattaaat aggttaagga acacgatgaa
     5581 cgcattatta agcaatccct ttaaagaacg tttacgcaag ggcgaagtgc aaattggtct
     5641 gtggttaagc tcgactactt cttatatggc agaaattgcc gccacttccg gttatgactg
     5701 gttgctgatt gacggagagc acgcgccaaa caccattcag gatctttatc accagctaca
     5761 ggcggtagcg ccctatgcca gccaacccgt gatccgcccg gtggaaggca gtaaaccgct
     5821 gattaaacaa gtcctggata ttggcgcgca aactctactg atcccgatgg tcgatactgc
     5881 cgaacaggca cgtcaggtgg taactgccac gcgctatcct ccctacggtg agcgtggtgt
     5941 tggggccagt gtggcacggg ctgcgcgctg gggacgcatt gagaattaca tggcgcaagt
     6001 taatgattcg ctttgtctgt tggtgcaggt ggaaagtaaa acggcactgg ataacctgga
     6061 cgaaatcctc gacgtcgaag ggattgatgg cgtgtttatt ggacctgcgg atctttctgc
     6121 gtcgttgggc tacccggata acgccgggca cccggaagtg cagcgaatta ttgaaaccag
     6181 tattcggcgg atccgtgctg cgggtaaagc ggctggtttt ctggctgtgg ctcctgatat
     6241 ggcgcagcaa tgcctggcgt ggggagcgaa ctttgtcgct gttggcgttg acacgatgct
     6301 ctacagcgat gccctggatc aacgactggc gatgtttaaa tcaggcaaaa atgggccacg
     6361 cataaaaggt agttattgat atcaaaggcc catggggatc ggctgtgggc ctgtgttaat
     6421 tagtggttat tcgctgccag atcggcttcg cttagctggg tggccgcgag cacctggtca
     6481 cgatcaaagc cctgttccag catgcgcaaa gcaattttaa tggcttgttc atgcaaacct
     6541 tctaatttac cttcctgcca gccaatttga tgtccttcct gccgcagccg ttcagcaata
     6601 gtcattaatc tctcctttta atgacagttc ggaatatcag catcagtgag ttgggttgtc
     6661 gccagcacaa tctcacgttc aaagccctgc tccagcatgc gcaaagcaat tttaatggct
     6721 tgttcatgca taccttcctg ccagccaatt tggtgtcctt cctgccgtag ccgctcagca
     6781 atagtcatca atctctcctt ttgtagtggt gaacgtttgg caatctcctc aataaaacgg
     6841 gtgaaacggg aggtatcgcc gcattgcagc agataattaa acagtgtttg tagctggctg
     6901 tcattagtga agcctttaac caaaagcgtg gtgatcctgt cgaccatacc gattaaatcg
     6961 cggtcgcgaa tatgcttttg aatcagttcc agcagagcta tacgccgatg ttgcatgatc
     7021 tcatcgtcag gtacgatggt gatatccacc aacggaaaag cttcggtgta caactgccgg
     7081 gcaagttgcg gatcgtcaaa ctcatccagc cagttaagtg agtacgggta gggcgatgtt
     7141 tcgccatgat aaaacagcaa tggcaccacc agcggaactc tgtcatagcc tttatccagg
     7201 tgacgctgca tggcggcagt ggcatagcgc attagccgaa aagccatatt cttttctgca
     7261 gagctttgat gttcaatcac gcagtagata tagccgtcac cgtcgctggt ttccacggac
     7321 cacaaaacat ccgagtagta agcgcgtaaa cttttttcaa taaaactagt gggttccagg
     7381 cgtaaggttt gcaggttgca aagcttgcgc agtggttctg gtaaatgtat ttcgagaaaa
     7441 tcccgtgcgg tttcgggtgt gaacataaag gttttaaata ccgcatcatg cggggaggag
     7501 gttgttgatt cggtcatggt tgtccgtcac ctgatattta tgatgacgcg acattacggt
     7561 tatcccaggc ctttgacatc tgccagtttc agcctttgcg agacgttttc agaggaattt
     7621 tcactgcgtt gcaacgcata cattgggcac ggaatcgtgt acgcaaaaat gcccggtaac
     7681 cacatgctta ccgggcatca gaaaggagtt taagccagcg cctgggccag tagcgtaatc
     7741 ggatgttcgc agcgaagact tgtggacatc tcaatctgcc atttacaggt ttcgcagtcg
     7801 gtgaccacca gatctgcgcc gctttcttct atctggcgga acagtggtgc gccgatggct
     7861 tgtgaggtgg ggtagttctc ttttttgaaa ccgtaagtac ccgcaatacc gcagcactgg
     7921 gaatccagca ccgttaactc aagccccggg attttacgca acagctccag ggtgtagagc
     7981 gtccagccca ttttttccat atggcacgga gtgtgataaa ccactttcag cggcagcggt
     8041 ttcagcggta acgttttgcc ttcgtccagc ttgcgccaca gccagcgggt tgccagttcg
     8101 atatgatcgc gcaaaccttt gttgtcgaca ttcagcactt ccggatattc gtcacgcagg
     8161 gcaaatgtac aggtcgagga ggtggcaatc accggaatgc cttttactcc cacagcttcg
     8221 cggatcgact ctacattcgt aattgcctgt ttgcgtgctt tagcggtaaa gccgttggcg
     8281 atcagcggta cgccgcagca tttttctttg ctgagcagtt gtacaccggt acccattgcg
     8341 ttgagcactt taattaaatc tttacctaac tgcggatggt tgtagttaac gaagcagccg
     8401 tgaaagaaag cgacctggtc tttatattgt gcttgctgag ccgccacgct gcgataccag
     8461 cgacggaacg tgccgaagga gtatttcggt agcgtgcggc gatgatcgat ttttaacgcc
     8521 gcatcaagca gctgccgcac cggtttcagc gaggtagcgg tgttgacgat tggtgcgaac
     8581 ggcgtggaaa cgctacccat caggtcggta tgactcaaca caaaattacg cagcgacggg
     8641 cgcgtggtgt catatttcgc ccgcgcgcgc tggataatat cgccaatctt cacatcggac
     8701 gggcaggcga cttcacaacg tttgcagttg atgcaatatt tcagcgcctc gtcatacagt
     8761 gcgccatctt tcaaacgcag acgctcgcca tccggcccgg cttgttttgg ccctggataa
     8821 ccgggattca cccggctcac cgggcaggcg gtggtgcaga cggtgcactt aatgcagttt
     8881 tcgaagctgg tgtcattcat tgttggcctc ctgcgcgttg ggcaatctgt tgagcggcat
     8941 gtaaagcact gacggcacaa acaccgccgc cgcatccctg ggcgatggga tcaaatccgc
     9001 ccagcaccga accgatggca aacaggtttt caatggtttg ccctgcctgt gacgggcgta
     9061 gcgtctcatc agtggttaca ccgaactgct gccacggttg cggcgcaaaa aaatctccct
     9121 tataccattc accccgcgtg gcggtttgta gcacatcaag gccgagaatc ggctctcgaa
     9181 tgccgttacg ttccgctacc agtccgccac taaagaaact gccgctggcg agaaccgcga
     9241 aacgtggacg tagcggaata tcggcgtgat tgcgggtcca gatttcgttc actacgccat
     9301 ttttacaggt cacttttttc acttcatcgc ccggcatcca cacgccaccc tggcgcacaa
     9361 actggcgctg taactggttt tgcagacgaa tgcccagcac ggaaggcggc agcgttggca
     9421 aaagcatcag tgaacaaggt agtttttcat tcaaccaacg ccacagtttg tcatcagcca
     9481 gaccgaagca ggcgggcatc aggatcattt cgcaggtatt ggcgacagga ataagcgcat
     9541 caagtaacag cggccagttt tcttcattat caaggaaacg ggcgatattc acagcgcgaa
     9601 attcggtggc gttattgcgc aggacatcca gttccggcag ctctatttct gcggtttcaa
     9661 ccgaaagatc cagttcgcgc aacgaagccg ccgcaagatg cgcctgaaaa tccatcaggc
     9721 cgcttatccc gaccacacat attttcttcg cgggcaacgg ccagacgggg acttctggcg
     9781 aacttagcca ggtagagcgc agagtgccga gcggcgtaat ccgctggtga gccagttcta
     9841 cgctgccctg caattgcgca ccgctttcag cgattaatgc ctgcgcctgg caagcgagat
     9901 cgagcacgcg ttgtggcccg agaagagagt aaggatgtgc aggtgcctgc tgacgcaaag
     9961 attccagtcc actgtgaatg tctgccacgg gttgaccatc tggcagatgg ctcagcaaat
    10021 ccagcgaacc ggatgagaaa tgcaacgcgc tttgaccacg cgtgacaatg gcacagcgca
    10081 ggccgtgttt ttgcagttgc aggccacaga gtaatccggc gaggccgccg cccataatga
    10141 cagtatcaaa gcgcatcttt ctgctccttc tccagaccac acaatccctg ataaacccag
    10201 cgggtaaatt cgctttcgcg cagtgcatct ccccaggcga tgggttgcac gcctttccag
    10261 cgttcgttaa ggaaggtgga aagttgctcg atagattgcg cggacgtcgt gacgttaaaa
    10321 cgttgcagca gtccggcagc gcggcaggcg cagagttcgc cctggcaggt gcccatcccc
    10381 acacgggtac gacggcgtaa atccagcagg ctattaacgt ttaaattttc taccgcgtac
    10441 tgcacttcac ccgcagttac cgcttcgcac tcacatacca ggctacggtg cagacggcct
    10501 tcgctcagcc aggcaggcgt gcgatcgcca tgacgataaa ccgcagaacc gcgcagcggg
    10561 gcaggcaggg agatgacttt acgcaaggta acttcagcgg ggtcttgcga accaggcagt
    10621 gccagatcgg cagtcgtaca ggggcgcgtg ttgcccagtt tgcggcatac cgcgtcggta
    10681 gcccattcag ccatcagccg ataggtcatc agtttgccac cggtgatggt gataaatccg
    10741 tccagaccat cgcgttcagc atggtcgagc agcacgatgc cacggctgac gttacgtccg
    10801 ctcgggtcgt catcgctggc aaccagcggg cgcacgccag aataggcccg caaaatgcgc
    10861 gttttcgcca tcacgggggc cagtttttcc ccttcacgca gcagaatatc aacctcttct
    10921 gccgtcactc gattatcgtc aatctcgttg taatcaatac gtaaagaggt ggtaccaatc
    10981 agcgaaatgg tatcgccagg caccagaata tcggcgtcgg aaggtttacg gcagcggttg
    11041 atcacatgct ggttaatgcg gtgatccatg atcagcagcg atcctttcgc cgggaacatg
    11101 cgaatgcgca gatcggcata ttcggcaatg tgttgccccc agatcccagc ggcattaacc
    11161 acgacaggtg catgaagggc ctgagtttcg ccggtgagat ggttacgtac acgaacaccg
    11221 cacaccgtcg cgccttcacg aatcagcccc gtgacttcat gagcggtaag gataacggca
    11281 ccgtgttctt tggcatccag catgtttgct gcggtcagac gaaatggatc aacggtgcca
    11341 tccggaactt tcaccgcgcc aatcagtgcc gggttaacgg caggttcgat aatgcgcgct
    11401 tgctgcgggt ctatagcttc tgcgctgatc cctgcttctt cgcaggcgcg aataaaagtg
    11461 gcctggaagg agaggtcatc ttccggcagg gtgataaaca ggccgttggt tggttcaacg
    11521 cagtgacgtg caatgcgttt caggatctgg ttttcactaa tgcattcgcg ggccgattcc
    11581 gcatcggtta ccgcatagcg cgcaccgctg tgcagcaggc cgtggttacg cccggtggca
    11641 ccggttgcga tgtcgtggcg ctcaaccaaa atcacgcgca gcccgcgcag ggcacagtcg
    11701 cgggcaatcc cggctcccgt tgcgccgccg ccaatgataa tcacgtcact tgattgcgag
    11761 tcgcgagttt tcattgttat ccctctgaag ttcgtttttt accatttagc catagtaaaa
    11821 acatgaattg tttgatttcg cgcatattcg ctcataattc gaaagtgaaa cgtgatttca
    11881 tgcgtcattt tgaacatttt gtaaatctta tttaataatg tgtgcggcaa ttcacattta
    11941 atttatgaat gttttcttaa catcgcggca actcaagaaa cggcaggttc tctcactgaa
    12001 tcaggctgtt aatcataaat aagaccacgg gccacggagg ctatcaatgt tgagtatttt
    12061 taaaccagcg ccacacaaag cgcgcttacc tgccgcggag atcgatccga cttatcgtcg
    12121 attgcgctgg caaattttcc tggggatatt ctttggctat gcggcttact atttggttcg
    12181 taagaacttt gcgcttgcta tgccttatct ggttgagcag ggattctcac gcggtgattt
    12241 aggttttgcc ctttcgggga tctcgattgc ttatggattt tcgaaattca tcatgggttc
    12301 ggtatcggat cgctcgaatc cgcgcgtttt cctgcccgca ggtttgattc tggcggcggc
    12361 agtgatgttg tttatgggct ttgtgccatg ggcgacgtcg agcattgcgg tgatgtttgt
    12421 actgttgttc ctctgcggtt ggttccaggg gatggggtgg ccgccgtgtg gtcgtactat
    12481 ggtgcactgg tggtcgcaga aagaacgtgg cggcattgtg tcagtgtgga actgtgcgca
    12541 caacgtcggt ggtggtattc cgccgctgct gttcctgctg gggatggcct ggttcaatga
    12601 ctggcatgcg gcgctctata tgcccgcttt ctgcgccatt ctggtggcat tattcgcctt
    12661 tgcgatgatg cgcgataccc cgcaatcttg tggtttgccg ccgatcgaag agtacaaaaa
    12721 tgattacccg gacgactata acgaaaaagc ggaacaggaa ctgacggcga agcagatctt
    12781 catgcagtac gtgttgccga acaaattgct gtggtatatc gccatcgcca acgtgttcgt
    12841 ttatctgctg cgttacggca tcctcgactg gtcaccgact tacctgaaag aggttaaaca
    12901 tttcgcgctg gataaatctt cctgggccta cttcctttat gaatatgcag gtattccggg
    12961 caccctgctg tgcggctgga tgtcggataa agtcttccgt ggcaaccgtg gggcaaccgg
    13021 cgttttcttt atgacactgg tgaccatcgc gactatcgtt tactggatga acccggcagg
    13081 taacccaacc gtcgatatga tttgtatgat tgttatcggc ttcctgatct acggtcctgt
    13141 gatgctgatc ggtctgcatg cgctggaact ggcaccgaaa aaagcggcag gtacggcagc
    13201 gggctttacc gggctgtttg gttacctggg cggttcggtg gcggcgagcg cgattgttgg
    13261 ctacaccgtg gacttcttcg gctgggatgg cggctttatg gtaatgattg gcggcagcat
    13321 tctggcggtt atcttgttga ttgttgtgat gattggcgaa aaacgtcgcc atgaacaatt
    13381 actgcaaaaa cgcaacggag gctaatggca tgaaattgaa gctgaaaaac cttagcatgg
    13441 cgatcatgat gagcactata gtcatgggaa gcagtgcaat ggcggcggac agcaacgaaa
    13501 aaatagtcat cgcccatcgc ggtgccagtg gatatttgcc ggagcatacg ctgccagcaa
    13561 aagcgatggc gtatgcgcag ggagcggatt atctggaaca ggatttggtg atgaccaaag
    13621 acgaccatct ggttgttctg catgaccatt atctcgatcg tgttactgat gttgccgatc
    13681 gtttcccgga tcgggcgcgc aaagacggtc gttactacgc gatagatttc acgctggatg
    13741 aaattaagtc gctgaaattt accgaaggtt tcgatattga aaacggtaaa aaagtacaga
    13801 cttatccggg gcgtttccca atgggtaagt ccgacttccg ggtgcacacc tttgaagaag
    13861 agattgaatt tgttcagggg ttaaatcact ctaccgggaa aaatatcggt atctatccag
    13921 aaatcaaagc gccgtggttc catcatcagg aagggaagga tattgcggca aaaacgctgg
    13981 aagtgctgaa gaaatatggt tacaccggta aggacgataa agtttatttg caatgttttg
    14041 atgctgatga gctgaagcgt attaagaatg agctggaacc taaaatgggc atggatctca
    14101 atctggtaca gctgattgcc tataccgact ggaatgaaac gcagcagaaa cagccggacg
    14161 gaagctgggt taattacagc tatgactgga tgtttaagcc aggtgccatg aagcaagtcg
    14221 cggaatatgc cgacggtatc gggccggatt accatatgtt gattgaggaa acatcgcagc
    14281 cgggtaatat caaactcact ggcatggtgc aagatgcgca gcagaataaa ctggtagtgc
    14341 atccttatac cgtgcggtcg gataaactgc ctgaatacac tactgatgtg aatcagttat
    14401 atgatgttct gtataacaaa gcgggtgtaa atgggctgtt tactgatttc cctgataagg
    14461 cagtaaaatt ccttaataaa gagtaatgct attgcaggat ggcgttcatg cgccatcctc
    14521 tgctaaagcg aaataatgct actaccgttg ttctattcat taaccggtgc atcctgtaaa
    14581 aaaaatgcca acatatttgc gtaaaactca ttcgttaggg aagctaacac tgggtattgt
    14641 tattctttaa acagtatgaa ttgggttcaa taattaattt tattgcagtt tatactgtaa
    14701 agtattattc gcatttcgga aaagtaaaga aaatattaat gtatcttatt aatttatcat
    14761 attgttcttg ggtgaatgct gtttgcacac cttcagggca atatttacca gtaataacca
    14821 gttgcccatt gctgtgttgt atcattaaac cttctttttc ctttggatct acaaacttag
    14881 aattatcaaa tgtgaagatg ttattttcct gttttgagta agcaaagaaa tctcgtgtat
    14941 agctgaaact atcatttatt aatggtgcta actttttgtc ggcaatatcg ataagctcac
    15001 cacctaattt at
//
LOCUS       DAFNBJ010000115        14986 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000115 DAFNBJ010000000
VERSION     DAFNBJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.115"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(69..2348)
                     /gene="maeB"
                     /locus_tag="L2X49_003422"
     CDS             complement(69..2348)
                     /gene="maeB"
                     /locus_tag="L2X49_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBN4798452.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2637..3587
                     /gene="tal"
                     /locus_tag="L2X49_003423"
     CDS             2637..3587
                     /gene="tal"
                     /locus_tag="L2X49_003423"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBN4798453.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3607..5610
                     /gene="tkt"
                     /locus_tag="L2X49_003424"
     CDS             3607..5610
                     /gene="tkt"
                     /locus_tag="L2X49_003424"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBN4798454.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5706..6749)
                     /locus_tag="L2X49_003425"
     CDS             complement(5706..6749)
                     /locus_tag="L2X49_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBN4798455.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6875..7450)
                     /gene="nudK"
                     /locus_tag="L2X49_003426"
     CDS             complement(6875..7450)
                     /gene="nudK"
                     /locus_tag="L2X49_003426"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBN4798456.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7518..9497)
                     /gene="aegA"
                     /locus_tag="L2X49_003427"
     CDS             complement(7518..9497)
                     /gene="aegA"
                     /locus_tag="L2X49_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBN4798457.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9703..11403
                     /gene="narQ"
                     /locus_tag="L2X49_003428"
     CDS             9703..11403
                     /gene="narQ"
                     /locus_tag="L2X49_003428"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HBN4798458.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11567..14680
                     /gene="acrD"
                     /locus_tag="L2X49_003429"
     CDS             11567..14680
                     /gene="acrD"
                     /locus_tag="L2X49_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBN4798459.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAISLVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14779..14838)
                     /gene="ypfM"
                     /locus_tag="L2X49_003430"
     CDS             complement(14779..14838)
                     /gene="ypfM"
                     /locus_tag="L2X49_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBN4798460.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3517 a         3979 c         3911 g         3579 t
ORIGIN      
        1 ttactaatat attgcagcca aaccgcttac cctgaatatt cagggtaagc gtgagagtta
       61 aaaaagaatt acagcggttg ggtttgcgct tcaaccacgg ccagcgccac catgttgacg
      121 atacgacgca ccgatgcgat cggcgttaac acgtgaaccg gtttcgccac gcccatcagc
      181 accggaccga cagtcacacc ttccgagctg gaaacgcgca gtaagttgta actaatgcgg
      241 gcagcttcca tgttcggcat caccagaata ttggcagaac ctttcaaagg gctgtccggc
      301 atacggtcgt tgcgaatcgc ttccaccagc gctgcatcgc cgtgcatttc accatcaatc
      361 atcagatctg gcgcacgatc cctgaccagt tccagcgcct gacgcatttt gctcgacgac
      421 gggcagtcag aagaaccaaa gttggagtgc gacaacagag caacgcgcgg ctcaatacca
      481 aaacgacgga cagtttctgc cgccatcaag gtgatctccg ccagctcttc tgcgtctggt
      541 tcgtcattga cgtaggtatc ggcaataaag gtgttaccac tcggcagcag cagcgcgttc
      601 attgcacctg cggtgtgaac gccatcgcga taaccaaaga catttttcac cacgctaaaa
      661 tgttcatgat aatcgcccac cgtaccgcag atcattgcat cggcttcccc acgctgaacc
      721 atgatcgcgc cgatcactgt cgggttactg atcagcgccc gctgcgcctg ttcctgagtg
      781 acaccgcgac gcttcatgat ctggaagtat tcggtccagt actctttaaa gcgcggatcg
      841 gattcgttat tgacgatctc aaaatcaacg cccgctttga tctgcaagcc cagtttctga
      901 atgcgcattt cgatcacgtt cggacgaccg ataaggatcg gtttcgccag tcccagcgtt
      961 accagttcct gagtggcatg cagaacgcgc gcctcttccc cttctggcag aacaacgcgc
     1021 ttcggcgctt tgcgagcctg ggagaaaatc ggcttcataa acaggttggt tttgtaaacg
     1081 aactcagtca gcttgtcgat gtagacgtcg aaatcagcaa tcggacgcgt cgccacgccc
     1141 gactccatcg cggctttagc gaccgcagga gcgatcttaa cgatcaagcg cggatcaaac
     1201 ggttttggaa tgatgtattc cggaccaaag ctcagatcct gatcgccata cgctgaagcc
     1261 accacttcgc tctgttcggc gtgggccagt tccgcaatcg cgcgtaccgc cgccagtttc
     1321 atctcttcat tgatagccgt tgcgccaaca tccagcgcgc cacggaagat aaacgggaag
     1381 caaaggacgt tgttgacctg gtttggatag tcagaacgac cggtgcaaat gatggcatcc
     1441 ggacgcactt ctttcgccag cggcggcaga atttccggtt ccgggttcgc cagcgccagg
     1501 atcattggcg cacgagccat tttcttcacc atttcctggg tcagcacttt cgggccggaa
     1561 catccaagga aaatatccgc gccttcaatc acatcatcga gggtacgttt gccgtcatcc
     1621 accaccgcat atgcggcttt ggtttccgcc atgtttggct cacggccctg atagataacg
     1681 ccttttgaat cgcaaaccac gatgttatgt ttttgcagac ccagcgctac cagcaggttc
     1741 atacaggcga ttgctgcggc acccgcgccg gaaaccacca tccgcacgtc ggagatgttt
     1801 ttctccacca cgcgcaagcc gttgaggatg gcggcagtgc tgatgattgc cgtgccgtgc
     1861 tgatcgtcgt ggaataccgg aatattcatc cgctcgcgca gtttctgttc aatatagaaa
     1921 cattctggcg ctttaatgtc ttcgaggttg attccgccga aggtcggttc gagcgcagcg
     1981 acaacttcaa taaatttgtc cgggtcgagt tcatcaactt caatgtcaaa tacatcaatc
     2041 ccggcgaatt tcttaaacag aacgcccttg ccttccatca ccggtttgcc agccagcgcg
     2101 ccaatgttgc ctaaccccag caccgccgta ccgttagaga tcaccgccac caggttaccg
     2161 cgcgcggtat atttgtaggc ttttaacggg tctttttcga tttcaagaca aggtgcggca
     2221 acgcctggtg agtaggccag cgccagatcg cgctgcgttg ccagaggctt ggttggagaa
     2281 acctggattt tccctggaac tggaaattca tggaaatcaa gtgcactttg ttttaactgg
     2341 tcatccattt ggttgttcct ttcacgtaac gttcacaaat aaagtgtgtg ggcaacagcc
     2401 cctgcccaca acgtggcgca cattattacc ctgccggagt ctacagactt tgagcaagtc
     2461 caaactctca ccattaatat aatgttttgg taataatcct ataacactga tgttacctgc
     2521 ttaatccagc aataccatgc ctgtctgcta tgcttttttg atgcgtttag cgaaatttct
     2581 cagaagtgtg aattaacgca ctcatctaac actttacttt tcaaggagta tttcctatga
     2641 acgagttaga cggcatcaaa cagttcacca ctgtcgtggc agacagcggc gatattgagt
     2701 ccattcgcca ttatcatccc caggatgcca ccaccaatcc ttcgctgtta ctcaaagctg
     2761 ccggattatc acaatatgag catttaatag acgatgctat cgcctggggt aaaaaaaatg
     2821 gcaagaccca ggaacaacag gtggtcgcag cgtgtgacaa actggcagtc aatttcggtg
     2881 ctgaaattct caaaatcgta cccggtcgcg tgtcaacaga agttgatgca cgcctctctt
     2941 ttgataaaga aaagagtatt gagaaggcgc gccatctggt ggacttgtat cagcaacaag
     3001 gcgttgagaa atcacgcatt ttgatcaaac tggcttcgac ctgggaagga attcgcgcgg
     3061 ccgaagagct ggaaaaagaa ggtattaact gcaacctgac gctgctgttt tcttttgccc
     3121 aggcgcgggc ctgtgcagaa gcgggcgttt ttctgatttc gccgtttgtc gggcgtattt
     3181 atgactggta tcaggcacgc aagccgatgg acccgtatgt ggtggaagaa gatccgggcg
     3241 ttaaatcggt gcgcaatatc tacgactact ataagcaaca ccactatgaa accattgtga
     3301 tgggcgcgag cttccgtcgc accgaacaaa tcctcgcctt aaccggctgc gatcgactga
     3361 ctatcgcacc gaatttgctg aaggagctgc aggaaaaagt ttcgccagtg gtacgtaaat
     3421 taatcccgcc ttctcagacg ttcccacgcc cagcacccat gagcgaagcg gagttccgtt
     3481 gggagcacaa tcaggatgcg atggcggtag aaaaactgtc tgaaggcatt cgtctattcg
     3541 ccgttgatca acgcaaactg gaagatcttc ttgccgccaa actataaacc agccacggag
     3601 tgttatatgt cccgaaaaga ccttgccaat gcgattcgcg cactcagtat ggatgcggta
     3661 caaaaagcca attctggtca tcccggcgcg ccgatgggca tggctgatat tgccgaagtg
     3721 ctgtggaacg attttcttaa acataatcct accgacccaa cctggtatga tcgcgaccgc
     3781 tttattcttt ccaacggtca cgcgtcgatg ctgctctaca gtttgctaca tctgaccggt
     3841 tacgacctgc cgctggaaga actgaagaac ttccgtcagt tgcattcgaa aaccccaggc
     3901 cacccggaga ttggctatac gccaggcgtt gaaaccacca ccggcccgct tggacaaggt
     3961 ttggcgaacg ccgtcgggct ggcgatagcg gaacgtacac tggcggcgca gtttaaccag
     4021 ccagaccatg agatcgtcga tcacttcacc tatgtgttta tgggcgacgg ctgcctgatg
     4081 gaaggtattt cccacgaagt ctgttcgctg gcaggcacgc tgggactggg caagctgatt
     4141 ggtttttacg atcacaacgg tatttccatc gacggtgaaa cagaaggctg gtttaccgac
     4201 gatacggcaa aacgttttga agcctatcac tggcatgtga tccatgaaat cgacggtcac
     4261 gatccgcagg cggtgaagga agcgatcctt gaagcgcaaa gcgtgaaaga taagccgtcg
     4321 ctgattatct gccgtacggt gattggcttt ggttcgccga ataaagcagg taaggaagag
     4381 gcgcacggcg caccactggg ggaagaagaa gtggcgctgg cacggcaaaa actgggctgg
     4441 caccatccgc catttgagat ccctaaaaag atttatcacg cctgggatgc ccgtgaaaaa
     4501 ggcgaaaaag cgcagcagag ctggaatgag aagtttgccg cctataaaaa ggctcatccg
     4561 caactggcag aagagtttac ccgacggatg agcggtggtt taccgaagga ctgggagaaa
     4621 acgactcaga aatatatcaa tgagttacag gcaaatccgg cgaaaatcgc tacccgtaag
     4681 gcttcgcaaa atacgcttaa cgcttacggg ccgatgctgc ctgagttgct cggcggttcg
     4741 gcggatctgg ctcccagcaa cctgaccatc tggaaaggtt ctgtttcgct gaaggaagat
     4801 ccagcgggca actacattca ctacggggtg cgtgaatttg gcatgaccgc tatcgccaac
     4861 ggcatcgcgc accacggcgg ctttgtgccg tataccgcga cgttcctgat gtttgttgaa
     4921 tacgcccgta acgccgcgcg gatggcggca ctgatgaaag cgcggcagat tatggtttat
     4981 acccacgact caattggcct gggcgaagat ggtccgacgc accaggctgt tgagcaactg
     5041 gccagcctgc gcttaacgcc aaatttcagc acctggcgac cgtgcgatca ggtggaagcg
     5101 gcggtgggct ggaagctggc ggttgagcgc cacaacggac cgacggcact gatcctctca
     5161 aggcagaatc tggcccaggt ggaacgtacg ccggatcagg ttaaagagat tgctcgtggc
     5221 ggttatgtgc tgaaagacag cggcggtaag ccagatatta ttctgattgc caccggttca
     5281 gagatggaaa ttaccctgca agcggcagag aaattagcag gagaaggtcg caatgtacgc
     5341 gtagtttccc tgccctcgac cgatattttc gacgcccagg atgaggaata tcgggagtcg
     5401 gtgttgcctt ctaacgttgc ggctcgcgtg gcggtggaag caggtattgc cgattactgg
     5461 tacaagtatg ttggtctgaa aggggcaatt gtcgggatga cgggttacgg ggaatctgct
     5521 ccggcggata agctgttccc gttctttggc tttaccgccg agaatattgt ggcaaaagcg
     5581 cataaggtgc tgggagtgaa aggtgcctga tggtgatcgc cggatgctga ttgccggatg
     5641 cgacgctgac gcgtcttatc cggcctacag cgtcttatcc ggcctacatg tccccgcaat
     5701 tttgtttaac gggtgatcca caacgtgggc caggcgtctg gcccatgcca gttatcgcag
     5761 gtgggttctg cggcataacg caccaggcga aaacgctgac cgtcaaagcg ccagcgcgcc
     5821 tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg tcaccagttc acgcgatttc
     5881 tcatcaaatg ttgcgttcat cagttccagt tcattcgtct cctgaccgtt gttgaacggc
     5941 aaacgcaacc gaaccgggcg cgaggctagt ggctttttac gcgacacaat ccatgccaaa
     6001 tcaatggtgt tataggcccc tgcctcacag ctaatcatca tcagcgcttt atcatcagtc
     6061 agcgcagtga cattcacctc acgacgtaac ggatcaagcg agcagcgcag accattcatc
     6121 cgccagtttc cataatccag caaatcgttg cgttcttcga gtgagagtgg cgtcggcgtt
     6181 gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg gtacgctgag cggcggttcg
     6241 tcccctttct tgatccacgc ggtttcactg ccaacgcgct tttgctgagc atcaataaac
     6301 aacaacgccg cttttaagcc actcagagaa atggtctgat tgccgtcgcg taaggtgatc
     6361 gccttccctt cctgaatcat ctgcaaaaac gcggtgatgg ttgccgtatc atcggttact
     6421 aataaccatg gtgaaatccg ccacttgtca ccacttaacg ccaacggctc accatctaac
     6481 agcagccgtg gcgctatctc tccttcagac gcatccggcg acttcaaccc gccgcgctca
     6541 atacgtaaaa cggcatcggt atgcgccccg gcgctgcggc tcagggtcat caccagtcca
     6601 ttatgatcgc ccgtattgcg cgccacgcag aagttttggt tattacaggt gacctgccag
     6661 tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag acgtcggcaa caaagcgaaa
     6721 aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga ccccagaagt tcaaacaaac
     6781 agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca atcagatttg tcggattgag
     6841 ccaataataa acaacgaaat attattgtta tttttcagtc cattaaatgt gacgtttgca
     6901 aatagttaag caataacacc gtcttaccgt cacgtatctc gccggttttg atcatctcca
     6961 gcgcctggct gaacggcagc tcgagcactt caatatcttc atcttcaaca ccgccaccgg
     7021 cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa gtggattagc tcagtcacac
     7081 cgccaggcga catatacagt tcaaataatt tgcgcacttc gcccacttca tagcccgtct
     7141 cttcaatcgc ttctttacga atgcacactt ccggttcgtc gttatccagc agcccggcgc
     7201 aggtttcaat cagctgcccg ctttcattgc cattaaccca ggtagcgaca cggaactgac
     7261 gaatcagaac cacggtcttt ttcttcgcgt tgtacaggag gatcgtcgca ccattaccgc
     7321 gatcgtaaac ttcacgttta tggcggataa cttcgccatc tttgcgcgtg agatcgtaag
     7381 taatgttgtg cagggtgaaa tagttatcgg agagaatttt gtctttaatg agggtgattt
     7441 gttgcgtcat agcgactcca cagcgcgaaa tgaacaatta tcttacgctg tgaagtcggg
     7501 tttgtctgcg caggctatca gtgagatttg actgatttta cccccagcca gtcaataatg
     7561 ccctgtgccg catgacgtcc ttctgccatt gcggtaacca ccagatccgc accacgcacg
     7621 gcgtcgccac cggcgaagat tttcggattg gtggtctggt aacggtactg gctttccaca
     7681 tccgcgatga tgcggcccca tttgtctacc gttacaccgt gcgactccag ccacggcatc
     7741 ccgtgtggat tgaagccaaa cgccataatc accgcatcgg ctggcatgac aaattcactg
     7801 ccctcaatcg gtacaggtcg ccgacgcccc tgggcatccg gctcgccaag acgcgtgcgc
     7861 aggaaacgaa ttccgcaaac gtgaccttgt tcattcagct caagcgccac cggctggacg
     7921 ttaaattcga agttggcccc ctcttcgcgg gcgttcttca cctctttctt cgagcctggc
     7981 atgttagctt catcacgacg ataagcgcag gtgacgttac tcgcgccgtg gcgcagcgca
     8041 gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta ccacgacgtt aagtccggcg
     8101 gtattgataa acggctcttc cggtagctct tcgagcccca tcacctgttt agtgttggca
     8161 atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt cattgggtaa acccgctttc
     8221 atggaacggt aagtgcctac gccaacgaag accgcgtcgt attgttccaa aagcgaatcc
     8281 aaagagacat ctttacccac ttcacaattg agttcgaagt gaatccccat cgcgctgaag
     8341 atttcccgac ggcgtgccag cagggattta tccagtttga aagaaggaat gccgaaagtg
     8401 agcaagccac caatttctgg atggcgatcg tacaccgtca ccgccacgcc attgcgggtc
     8461 agaacatccg cacaggccag ccctgccggg cctgccccga taatcgccac ccgcttgtcc
     8521 actttggtga catgacttaa gtcaggacgc caacctttcg ccaacgcctg atctgaaata
     8581 tagcgttcaa tgttgccgat agttacagca ccgtgctcat cgcgaatagt acaggcacct
     8641 tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt ccggcagggt gttggtctgg
     8701 tgagaaagct cgacggcggc gtcgatgttt ccggctttca ccagttcaat ccactgcgga
     8761 atatggttat gcagcgggca ggtccattca caaacgctgt gctcgccgca cttaaggcag
     8821 cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca gataaatttc atcaaaaccg
     8881 gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac gcgcgggcgt tgcctgcatt
     8941 tgttcgactt tactcattac cggcatttct tgcgccgcgg tactggcatg ccacggttga
     9001 tgttcctgac gcgcggtgcg caagcggcgg gatttcgcca tgccggagag tgcgacgtca
     9061 gtgaccagtt gcagcgcgtc cgccgggcaa ttctcaacac aggcaggacc gttttcgcgc
     9121 cccgcacaaa ggtcgcattt atgcgccgtg gcttttactt ttcctgccgc gacgggtgtc
     9181 aggacgattt gcatcgtacc aaaaggacag gccaccacgc aggatttaca gccaatacac
     9241 ttttgctgat tgacctgaat gctgtcatca acgtggctga ttgcgccatt agggcagcta
     9301 cgggcgcagg gcgcatcttc acaatggtga caggtcactg cactacgttg ctgttgatgt
     9361 ttgataaccg taattcgggg atgaaaatgg tgttggctca ggacatgttg ctcatcattg
     9421 tgagccatga cacaggcgat ttcacaagca tgacaaccca gacattgctg actgttggcc
     9481 ataataaaac gattcataac gaccttcttt tttggttgta aaaaccttat tctttatatg
     9541 agtgttgtta ttacccgact tacaggggaa tcggcaatgt tcatgtgccc agaataagta
     9601 actatttcgc tataaactgt ggcagatcaa ataatcccat cactgactaa attgtgtttc
     9661 agctaactgg aacattaatg attttttgtg gagaagacgc gtgtgattgt taaacgaccc
     9721 gtctcggcca gtctggcccg ggcctttttt tacattgtgc tgctgtcgat tctttccacg
     9781 ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg ctgaggctat caatattgcc
     9841 ggatcgctac gtatgcagag ttaccgcctg ggctacgact tgcaaagtgg cagtccacaa
     9901 ctcaatgcac atcgccagtt gtttcagcag gcactgcatt caccggtatt aaccaatctc
     9961 aacgtctggt atgtgccaga agcagtaaaa actcgctatg cgcatctgaa tgccaactgg
    10021 ctggagatga ataatcggct cagcaagggc gatttgccgt ggtatcaggc caatattaat
    10081 aattatgtta atcagataga cctgttcgtg ctggctttac agcactacgc tgaacgcaaa
    10141 atgctgctgg tggtggcgat ttccctggct ggcggtatcg gtattttcac gctggtcttt
    10201 tttactttgc gccgtatacg ccatcaggtg gttgccccgc tgaatcagct ggttaccgcc
    10261 agtcaacgta ttgaacacgg gcagttcgac tcgccgccgc tggataccag tctgccgaat
    10321 gagcttggtc tgcttgcaaa aacctttaac cagatgtcga gcgagctgca taaattgtac
    10381 ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc tccacgaggc caagcgtcgt
    10441 ctggaggtgt tgtatcaatg ttcccaggcg ctgaacacca gccagattga tgtgcattgt
    10501 ttccgccata ttttgcagat tgttcgcgat aatgaagcgg ctgaatatct ggagttaaat
    10561 gtcggtgaaa actggcggat tagcgaaggg caaccaaacc cggaattgcc gatgcagatt
    10621 ttaccggtga caatgcaaga gacggtttac ggcgaactgc actggcaaaa tagtcacgtt
    10681 tcatcatcag aaccgctgct taacagcgtt tcgtcgatgc tgggacgcgg tttgtacttt
    10741 aatcaggcgc agaagcattt tcagcagtta ttgttgatgg aagaacgtgc aaccatcgcc
    10801 cgcgaattgc acgactcgct ggctcaggta ctttcttact tacgtatcca gttgacgtta
    10861 ctgaagcgtt cgataccgga agataacgcc accgcacaaa gtatcatggc cgatttttcc
    10921 caggcgttga atgatgctta tcggcagtta cgcgagctgt tgactacttt ccgcctgacg
    10981 ctgcagcagg cggatctccc ctccgcgttg agggaaatgc tggatacgtt acaaaatcaa
    11041 accagcgcca aactgaccct cgactgccgt ctgccaaccc tggcgctgga tgcgcaaatg
    11101 caggtgcatt tgttgcaaat tattcgcgaa gcggtgctga atgcgatgaa gcacgccaac
    11161 gccagcgaaa tcgccgtcag ttgcgtcacc gcgccggacg gcaatcacac ggtttatatc
    11221 cgtgataacg ggattggtat cggtgaaccg aaagaacccg aaggtcatta tggtctgaat
    11281 atcatgcgcg aacgcgcgga acggctgggt gggacgctga ctttttcaca accttccggc
    11341 ggcggcacgt tagtgagtat tagctttcgc tccgcggagg gtgaggaaag tcagctgatg
    11401 taatgcctcc tactgaccaa agaatacttg cacttaaggt tcagtataaa agggcatgat
    11461 aatttacatt aactcctttt tttctccacg attggctcgt accttgccgc tacagtgaag
    11521 caagtcaagc ctacaacgat acgcagaaac acgaggtcct cttttaatgg cgaatttctt
    11581 tattgatcgc cccatttttg cctgggtgct ggcaatcctg ttgtgtctga caggtaccct
    11641 ggcgattttc tcattgcccg ttgaacaata ccccgatctc gcgccaccga atgtgcgagt
    11701 gaccgctaac tatcccggcg catcggccca gacgctggaa aacaccgtga cccaggttat
    11761 cgagcaaaat atgaccggcc tcgataatct catgtatatg tcatctcaga gcagtggcac
    11821 cggtcaggca tctgtcactt taagttttaa agcaggcacc gatccggacg aagccgtgca
    11881 gcaagtacaa aaccagctgc aatcagccat gcgaaagtta ccgcaggcgg tgcaaaatca
    11941 gggcgtgacg gtgcgtaaaa ccggcgatac caatattctg accattgctt tcgtctctac
    12001 cgatggttcg atggataaac aggatattgc cgattatgtt gccagtaata ttcaggaccc
    12061 gttaagccgc gtgaatggcg tcggggatat cgatgcctat ggttcgcaat attccatgcg
    12121 tatctggctg gacccggcga aactcaacag tttccagatg acggctaaag atgtcactga
    12181 tgccattgag tcacagaacg cgcagattgc ggttgggcaa cttggtggta caccttccgt
    12241 cgataagcag gcgctcaacg ccaccattaa cgcccagtca ctgctgcaaa caccagaaca
    12301 gttccgcgat atcaccttgc gggtgaatca agacggctca gaggtaaggc tgggcgatgt
    12361 cgccaccgtc gaaatggggg cggagaaata cgattatctt agccgcttca atggtaagcc
    12421 agcctccggg ctgggggtaa aactggcctc cggcgcaaac gaaatggcga cagcggagct
    12481 ggtgctcaat cgtctcgacg agctggcgca gtacttcccg cacggactgg aatacaaggt
    12541 ggcgtatgaa accacctcgt ttgttaaagc ctccattgaa gacgtggtga aaacgctgct
    12601 ggaagctatc tctctggttt tcctcgttat gtatctgttc ctgcaaaact tccgcgccac
    12661 gctgataccc actatcgccg tgccggtggt gttgatggga accttctccg tactttacgc
    12721 cttcggttac agcgtcaaca ccttaaccat gttcgcgatg gtgctggcga tcggtctgct
    12781 ggtggatgac gccatcgtgg tggtggaaaa cgtcgaacgt attatgagtg aggaaggact
    12841 cactcctcgc gaagccacac gtaaatcgat ggggcagatc cagggggcac tggtcgggat
    12901 tgcgatggtt ctttcggcgg tatttgtacc aatggccttc ttcggcggca ccaccggtgc
    12961 catctatcgc cagttctcta ttaccattgt tgcggcgatg gtgctgtcag tactggtagc
    13021 gatgatcctc actccggctc tgtgtgccac actacttaag ccactgaaaa aaggtgagca
    13081 tcacgggcaa aaaggctttt ttgcctggtt taaccagatg tttaaccgca acgccgaacg
    13141 ctacgaaaaa ggggtggcga aaattctcca ccgtagcctg cgctggattg tgatttatgt
    13201 cctgctgctt ggcggcatgg tgttcctgtt cctgcgtttg ccgacgtcgt tcttaccgct
    13261 ggaagaccgt ggcatgttta ctacctcggt acaattgccc agcggttcaa cccaacaaca
    13321 gaccctgaaa gtcgttgagc aaatcgagaa atattacttc acccatgaaa aagacaacat
    13381 catgtcggtg tttgccactg ttggttctgg ccccgggggt aacgggcaaa acgtggcgcg
    13441 aatgtttatc cgcctgaaag actggagcga acgcgacagt aagaccggca cctcgtttgc
    13501 cattatcgag cgtgcaacaa aggcgtttaa caagattaaa gaagctcgcg ttatcgccag
    13561 cagcccgcca gcaattagcg gtcttggtag ttctgcgggt tttgatatgg agttgcagga
    13621 ccacgctgga gcaggtcacg atgcgctgat ggcagcacgt aaccagttgc tggcgctggc
    13681 ggcggaaaac ccggagctaa cccgtgtgcg ccataacggc ctcgacgaca gtccgcagtt
    13741 gcagattgat atcgaccagc gtaaagctca ggcgctgggc gttgatatcg acgatattaa
    13801 cgacacactg caaaccgcct ggggttcgag ctatgttaat gactttatgg atcgcggtcg
    13861 cgtgaagaaa gtctatgtgc aggcagctgc gccgtatcgc atgctgccag atgacatcaa
    13921 tctctggtat gtccgaaata aagatggcgg catggtgccc ttctctgctt tcgcgacctc
    13981 acgctgggag acaggctcgc cgcgtctgga acgctataac ggttattctg cggttgagat
    14041 tgttggggaa gccgcaccgg gggtcagtac cggtacggcg atggatatta tggaatcgtt
    14101 agtgaagcag ttgccaaacg gctttggtct ggagtggacg gcgatgtcgt atcaggagcg
    14161 gctttccggc gcgcaggctc cggcgctgta cgctatttcc ttgctggtgg tattcctgtg
    14221 tctggcggcg ttgtatgaaa gctggtcggt gccgttctcg gtaatgctgg tcgtgccgct
    14281 gggggttatc ggcgcgctgc tggcaacctg gatgcgcggg ctggaaaacg acgtttactt
    14341 ccaggtgggc ctgttaacgg tcattggttt atcggcgaaa aacgccatcc tgatcgtcga
    14401 gtttgctaac gagatgaacc aaaaaggcca cgacctgttt gaagcgacgc tccacgcctg
    14461 ccgtcagcgt ttacgcccga ttctgatgac ctcgctggca tttatcttcg gcgtattgcc
    14521 aatggcaacc agcacgggtg ccggttccgg tggtcagcat gcggtgggta ctggcgtaat
    14581 gggcgggatg atttcggcca ctattctggc tatttacttc gtgccgctgt tctttgtact
    14641 ggtgcgccgc cgcttcccgc tgaagccgcg cccggaataa gcaataaaaa aggcgacatg
    14701 ctaatgtgtc gcctttttca actttccgat taagaacctg ctcagcgggt tcttgctgtt
    14761 tgtactttgt ctcaggaatt acttacgaag cataacttcg ataaagtctt tccagttccc
    14821 cagttcacgt tcaatcataa caacctctct tataattatg ggtattctac ggaaacaata
    14881 taccgtggtg aagctaattt actcgattgc tgcgatgact acctccgggg gacaaacctt
    14941 atgtaaatac tatggtcctg cggtgataat ttgtatgtga tacaca
//
LOCUS       DAFNBJ010000116        14809 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000116 DAFNBJ010000000
VERSION     DAFNBJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.116"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..759
                     /locus_tag="L2X49_003431"
     CDS             <1..759
                     /locus_tag="L2X49_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBN4798461.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            915..2450
                     /gene="gadC"
                     /locus_tag="L2X49_003432"
     CDS             915..2450
                     /gene="gadC"
                     /locus_tag="L2X49_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBN4798462.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2581..3900
                     /locus_tag="L2X49_003433"
     CDS             2581..3900
                     /locus_tag="L2X49_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBN4798463.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4198..4239
                     /locus_tag="L2X49_003434"
     CDS             4198..4239
                     /locus_tag="L2X49_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798464.1"
                     /translation="MVQLVDLARCVSF"
     gene            4277..5659
                     /gene="dosC"
                     /locus_tag="L2X49_003435"
     CDS             4277..5659
                     /gene="dosC"
                     /locus_tag="L2X49_003435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBN4798465.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5684..8083
                     /gene="dosP"
                     /locus_tag="L2X49_003436"
                     /gene_synonym="pdeO"
     CDS             5684..8083
                     /gene="dosP"
                     /locus_tag="L2X49_003436"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HBN4798466.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKETISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8341..8922
                     /gene="ddpX"
                     /locus_tag="L2X49_003437"
     CDS             8341..8922
                     /gene="ddpX"
                     /locus_tag="L2X49_003437"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HBN4798467.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQVCPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHDRSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8936..10486
                     /locus_tag="L2X49_003438"
     CDS             8936..10486
                     /locus_tag="L2X49_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN4798468.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTSVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYS
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10488..11510
                     /locus_tag="L2X49_003439"
     CDS             10488..11510
                     /locus_tag="L2X49_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4798469.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQHLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFSATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLSGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11507..12403
                     /locus_tag="L2X49_003440"
     CDS             11507..12403
                     /locus_tag="L2X49_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBN4798470.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12400..13386
                     /locus_tag="L2X49_003441"
     CDS             12400..13386
                     /locus_tag="L2X49_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN4798471.1"
                     /translation="MTQPVLDIQQLHLSFPGFHGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIDLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13379..14305
                     /locus_tag="L2X49_003442"
     CDS             13379..14305
                     /locus_tag="L2X49_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN4798472.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRV
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(14361..14792)
                     /gene="osmC"
                     /locus_tag="L2X49_003443"
     CDS             complement(14361..14792)
                     /gene="osmC"
                     /locus_tag="L2X49_003443"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBN4798473.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3570 a         3713 c         3797 g         3729 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgatacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tactttcgcc
      301 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
      361 ggtcgtgaag gctacaccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
      421 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta ccggtcgccc ggacgaaggc
      481 attccggcgg tttgcttcaa actcaaagat ggtgaagatc cgggatacac cctgtatgac
      541 ctctctgaac gtctgcgtct gcgcggctgg caggttccgg ccttcactct cggcggtgaa
      601 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
      661 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
      721 cagggtattg cccagcagaa cagctttaaa catacctgat aacgtttaac ggtaacggtg
      781 tcccgaaacg aacccgtttc gggacaattt ccaaagtctg ttcactggca ttagcaacgg
      841 aaaatattgt tctgaatacg cttcagaaca aaacaggtgc ggttccgaca ggaataccgt
      901 tttaggggga taatatggct acatcagtac agacaggtaa agctaagcag ctcacattac
      961 tcggattctt tgccataacg gcatcgatgg taatggctgt ttatgaatac cctaccttcg
     1021 caacatcggg cttttcatta gtcttcttcc tgctattagg cgggatttta tggtttattc
     1081 ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa ggtggtgtct
     1141 tcgcctgggt atcaaatact ctggggccga gatggggatt tgcagcgatc tcatttggct
     1201 atctgcaaat cgccattggt tttattccga tgctctattt cgtgttaggg gcactctcct
     1261 acatcctgaa atggccagcg ctgaatgaag accccattac caaaactatt gcagcactca
     1321 tcattctttg ggcgctggca ttaacgcagt ttggtggcac gaaatacacg gcgcgaattg
     1381 ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt tattttgatc gcattagcgg
     1441 ctatttatct gcactccggt gcccccgttg ctatcgaaat ggattcgaag accttcttcc
     1501 ctgacttctc taaagtgggc acactggttg tatttgttgc cttcattttg agttatatgg
     1561 gcgtagaagc ttccgcaact cacgtcaatg aaatgagcaa ccccgggcgc gactatccac
     1621 tggctatgtt actgctgatg gtggcggcaa tctgcttaag ctctgttggc ggtttatcta
     1681 ttgcgatggt cattccgggt aatgaaatca acctctccgc aggggtaatg caaaccttta
     1741 ccgttctgat gtcccatgtg gcaccggaaa ttgagtggac ggttcgcgtg atctccgcac
     1801 tgctgttgct gggtgttctg gcggaaatcg cctcctggat tgttggtcct tctcgcggga
     1861 tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg aacaaaaatg
     1921 gcgtaccggt aacgctggtc atttcgcagc tggtgattac ttctatcgcg ttgatcatcc
     1981 tcaccaatac cggtggcggt aacaacatgt ccttcctgat cgcactggcg ctgacggtgg
     2041 tgatttatct gtgtgcttat ttcatgctgt ttattggcta cattgtgttg gttcttaaac
     2101 atcctgactt aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa ttggtcgtgg
     2161 caattgtcgg tctgctgact tcaattatgg cgtttattgt ttccttcctg ccgccggata
     2221 acatccaggg tgattctacc gatatgtatg ttgaattact ggttgttagt ttcctggtgg
     2281 tacttgccct gccctttatt ctctatgctg ttcatgatcg taaaggcaaa gcgaataccg
     2341 gcgtcactct ggagccaatc aacagtcaga acgcaccaaa aggtcacttc ttcctgcacc
     2401 cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa gaaacactaa ccatgtctga
     2461 atggttataa gacaagggag cgataagtca tcgctccctt tttcgtgctt gctgtcgtat
     2521 tgacttaacc gggataagta cgagaatgag cgcacatctg tttaccggaa accagcacat
     2581 atggatatct gctcccgaaa caagaaatta acgattagaa gaccagcgat actagttgca
     2641 ctggcacttt tactgtgtag ttgtaaaagc acgcctccag agtccatggt gacaccacca
     2701 gcaggttcaa agccaccagc cacgacgcaa caatcgtcac aaccgatgcg tggcatctgg
     2761 ctggccacgg tttctcggct cgactggcca ccggtttcct cggttaacat tagtaacccc
     2821 accagccggg cccgtgtaca acaacaggcg atgatcgaca aactggatca tctgcaacgt
     2881 ctcggcataa acacggtctt tttccaggtc aagccggacg gtaccgccct gtggccatcg
     2941 aaaattttgc cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc gggttacgat
     3001 ccgctgcaat ttatgctcga cgaagcccac aagcgtggga tgaaagtaca cgcctggttt
     3061 aacccctatc gcgtatcggt taatacgaag cccggtacta tcagggaact gaatagcact
     3121 ctgtctcaac aaccggcgag cgtctatgtg caacaccgcg actggatcag aacgtctggc
     3181 gatcgctttg tcctcgaccc gggcatccct gaggttcagg actggatcac atcaatagtc
     3241 gcagaagtgg tttcccgcta tcccgtagat ggcgtgcagt ttgacgacta tttctatacg
     3301 gagtcaccgg gttcacggct aaatgataac gaaacgtacc gtaaatacgg aggcgcattt
     3361 gcgtcaaaag cagactggcg gcgcaacaat actcagcagt taattgcaaa ggtatcgcac
     3421 accattaaaa gcattaagcc gggagtcgaa tttggtgtta gcccggcagg cgtgtggcgt
     3481 aaccgatcac acgatccgct cggttccgat acccgaggcg cggcagccta tgacgaatcc
     3541 tacgctgaca cccgtcgatg ggtggaacaa ggattgctgg attacattgc tccccaaatt
     3601 tactggccgt tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg gtgggcggat
     3661 gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg ccttctataa agtgggtgaa
     3721 ccctcaaaga tagagccaga ctggatgatt aacggcggcg taccggaact gaaaaagcag
     3781 ctcgatctta acgatgcggt accagaaatt agcggcacca tcttgttccg tgaggactat
     3841 ctgaataaac cgcagactca acaagcggtc agctatctgc aaagtcgttg gggcagttaa
     3901 actcccattc ttactttgta acaagccggt gctttaccca ccggcttgtt accctcttga
     3961 actatcccac tactaatcat gcttactaaa gtcaaattaa ccacgcaatt aaccacactt
     4021 agttaaacag ccataaatcc cctaataaaa atgtatgaat atgaaaaaac atacgatatt
     4081 cgctgtcacc gaaaaataat tacgatgggc tatactcttc tgaagaacag tagcgtagat
     4141 taaaactgtc ctaagtcgct tatttctttt cagtatatct tcatatttca ggagaatatg
     4201 gtgcagttag tcgatctcgc acgttgcgtt tcgttttgat tccgtctgat ttcccagcct
     4261 tataagggtg tgaattatgg agatgtattt taaaagaatg aaagatgagt ggaccggact
     4321 tgtcgaacag gcagatcctc tcattcgtgc taaagccgca gaaattgccg ttgcgcatgc
     4381 tcattatctg agtatcgagt tttatcgaat tgtccgcatc gacccgcatg ccgaagaatt
     4441 cttgagtaat gaacaagttg agcggcagtt gaaaagtgcg atggaacgct ggattattaa
     4501 cgtgctttct gcccaggttg acgatgtcga aaggctaata caaatccagc ataccgtcgc
     4561 ggaagtgcat gcccgcatag gaattccggt agaaattgtc gagatggggt ttcgggtgct
     4621 gaaaaaaatc ctctatccgg taatcttctc ttcggattat tccgccgcag aaaaacttca
     4681 ggtctatcat ttctcgatta acagtattga tatcgcgatg gaagtgatga cccgcgcgtt
     4741 tacctttagt gacagtagtg cctcaaagga agatgaaaac tatcgtatct tctcgttgct
     4801 ggaaaacgcc gaagaagaaa aagaacggca aatagcctca atactttcat gggaaataga
     4861 tattatctat aaaatcctgc tggattctga tttaggcagt agtttgcctt taagccaggc
     4921 tgattttggc ctgtggttta accataaagg tcgacattat tttagtggca ttgccgaagt
     4981 aggccatatc tcccgtctga ttcaggattt cgacggtata ttcaatcaaa ccatgcgtaa
     5041 caccaggaat ctgaataaca gaagtctgcg ggtgaaattt ttattacaga taagaaatac
     5101 tgtatcgcaa attattacct tgctgcgtga attgtttgaa gaagtatcgc gccacgaagt
     5161 cggtatggat gtactgacga aattacttaa ccgccgtttc ctgccgacta tcttcaaacg
     5221 cgaaattgcc catgccaacc ggaccggtac accgctgtca gtgctgatta ttgacgttga
     5281 taaattcaaa gagatcaacg atacgtgggg ccataacact ggcgatgaaa ttctgcgtaa
     5341 agtctctcag gccttttatg acaacgtccg cagtagtgat tatgttttcc gctacggggg
     5401 cgatgaattt atcattgttt tgactgaagc ttcggaaaac gaaacgttac gtaccgcaga
     5461 acgtattcgc agtcgggtgg agaaaaccaa actgaaagcc gcaaacggcg aggatattgc
     5521 cctctcactt tccatcggtg ccgccatgtt taatggtcat cctgactatg agcgcctcat
     5581 tcaaatagcc gatgaagctc tgtatatcgc caaaagacga ggtagaaacc gtgttgaact
     5641 ctggaaagcc agtctttaga tgcgccagga tgcagaggta atcatgaagc taaccgatgc
     5701 ggaaaatgcc gccgatggca tttttttccc cgcccttgag caaaatatga tgggtgcggt
     5761 gttaattaac gaaaatgatg aagtgatgtt tttcaacccc gccgcagaga agctctgggg
     5821 gtacaaacgt gaagaagtca ttggcaataa cattgatatg ctgattccgc gggatttgcg
     5881 tcctgcgcat cctgaataca ttcgtcataa ccgtgaaggc ggtaaagcgc gcgtcgaggg
     5941 gatgagtcgg gagctgcagc tggagaaaaa agacggcagt aaaatctgga cccgttttgc
     6001 gctatcgaaa gtgagcgccg aggggaaagt ttattacctg gcgctggtac gggatgccag
     6061 cgtagaaatg gcgcaaaaag aacagacccg acaattgatt attgccgttg accatctcga
     6121 ccgaccggtg attgtcctcg atccggaacg ccatattgtg cagtgcaatc gcgcatttac
     6181 cgaaatgttt ggttactgca ttagcgaagc cagcggtatg cagcccgata cactcctgaa
     6241 cattcctgaa ttccctgccg ataaccgcat tcgtttacaa cagttgctat ggaaaaccgc
     6301 ccgcgatcag gacgaatttc tgctgttgac gcgcaccggt gaaaaaatct ggattaaagc
     6361 ctctatcagc ccggtttatg acgtgctcgc gcatctgcag aacctggtaa tgactttctc
     6421 ggatatcacc gaagaacggc agattcgcca gcttgaaggc aatattctcg ccgccatgtg
     6481 cagcagcccg ccatttcatg aaatggggga aatcatttgt cgtaacatcg aatctgtact
     6541 caacgaatcg catgtttcgc tgttcgcact gcgcaacggg atgccgatac actgggcgtc
     6601 atcttcgcac ggtgcagaag ttcaaaatgc gcaaagctgg tcagcgacca ttcgtcagcg
     6661 tgatggcgcg cctgcgggga tcctgcaaat taaaacctcg tcaggagcag aaaccagcgc
     6721 ctttatcgaa cgcgtggcag atatcagcca gcatatggcc gcgctggcgc tggaacagga
     6781 aaaaagccgt cagcatattg aacaactcat ccaatttgat ccgatgaccg gtctgccaaa
     6841 tcgcaataac ctgcacaatt acctcgatga cctggtcgac aaagccgtct ctccggtggt
     6901 gtatctcatc ggtgttgacc atattcagga tgtgattgat agccttggct atgcgtgggc
     6961 cgatcaggca ttgctggaag tggtcaatcg ctttcgtgaa aaactcaaac cggatcagta
     7021 tctctgtcgt atcgaaggta cgcagtttgt cctcgtgagc ctggaaaacg acgtcagtaa
     7081 cattacccag atcgccgatg agctacggaa tgtggtcagc aagccgataa tgattgacga
     7141 taaacccttc ccgcttacct tgagtattgg catcagctac gacgtgggga aaaaccgcga
     7201 ttacctgctc tccactgccc ataacgcgat ggattatatt cgcaagaatg gcggtaacgg
     7261 ctggcagttc ttcagcccgg cgatgaacga aatggtaaaa gagcgtttgg ttttaggcgc
     7321 agcgctgaaa gaaacgatta gcaataacca actgaaactg gtttaccagc cgcaaatctt
     7381 cgcagaaacg ggtgaactgt acggcatcga agcccttgct cgctggcacg atccccagca
     7441 tggtcatgta cccccttcac ggtttattcc tctcgcagaa gagattggtg aaatcgaaaa
     7501 tattgggcgc tgggtcatcg cggaagcttg ccgtcagtta gcagaatggc gtagccagaa
     7561 tattcatatc ccggcgttat ccgtgaactt gtcggcgctg cactttcgca gtaatcaact
     7621 gcctaatcag gtgtctgatg caatgcaagc ctggggtatt gacggccacc agctgacggt
     7681 ggaaatcacg gaaagcatga tgatggaaca cgataccgaa atctttaagc gcattcagat
     7741 cctgcgtgat atgggcgtgg gtttatcggt agatgatttt ggcacgggct tttccggatt
     7801 atcccgctta gtcagtcttc cggtaacgga aatcaaaatt gacaaaagtt ttgtcgatcg
     7861 ttgtctgacg gaaaaacgca tccttgcctt acttgaagcc attaccagca ttgggcaaag
     7921 cctcaattta accgtcgtgg cggaaggcgt cgaaaccaaa gagcaatttg agatgctacg
     7981 caagatccac tgtcgcgtta ttcagggata tttcttttcc cgccccctgc ccgccgaaga
     8041 aattccaggc tggatgagca gcgtgttacc gctgaaaatc tgacaaattc ctctagcccg
     8101 cactcgcggg tattcattta acgtgacact gtcacttaaa tactgtgatt tcagctacga
     8161 ttccgggaga ttccttcctt aacttcccct cttgcaccaa aactgcacta cgctgcacaa
     8221 atgttgatca aaagttaaaa aatataactt taagccagca ttctggcgct tctcccggca
     8281 tggcatgaga tctgcataag cggaaagcgc agcaattttt gtcttataca ggaaccgttt
     8341 atgtcggata ccaccgaact ggttgattta gccgtaatct tccctgatct ggagatcgaa
     8401 ttgaaatacg cctgcgctga taacatcaca ggtaaagcta tttatcagca agcgcgttgt
     8461 ctgttacaca aggatgcgat taccgcgctg gcgaaaagta tcagtatcgc ccagctgtca
     8521 ggcttacaac tggtgattta cgatgcgtat cgcccacaac aagcacaggc gatgttgtgg
     8581 caagtctgcc cagacccgca atatgttgtt gatgtgacgg tcggttctaa tcacagccgt
     8641 ggcacggcga tcgacctgac gcttcgtgat gagcacggga acatcctcga tatgggcgca
     8701 gggttcgatg aaatgcacga tcgctcccat gcttaccatc cttccgtccc gcccgccgct
     8761 cagcgcaatc gcctgttgct gaatgcgatt atgactggtg gcggttttgt cggtatctcc
     8821 agcgaatggt ggcacttcga attaccccag gcagcgagtt acccactgct cgctgatcaa
     8881 ttcacctgtt ttatatcccc tggcacacag cacgtcagtt aatcaggaaa ctgtcatgaa
     8941 aagatcgata tcgtttcgtc ctacattgct cgcgctcgtc cttgccacaa ctttcccggt
     9001 tgcgcacgcc gccgtaccga aagatatgct ggtgatcggt aaggctgccg atccacaaac
     9061 cctcgacccg gcggtgacaa ttgataataa cgactggacg gtgacctacc cgtcttatca
     9121 gcggctggtt cagtacaaaa cggacggtga taaaggctca accgacgttg aaggcgatct
     9181 ggcaagtagc tggaaagcgt ctgacgatca aaaagagtgg acgttcaccc tgaaggataa
     9241 tgctaaattt gccgatggca catctgtcac tgccgaagca gtaaaactct cttttgagcg
     9301 gttactaaaa atcggccagg ggccagcaga agcatttccc aaagatttaa agattgatgc
     9361 tcccgacgaa catacggtga agtttaccct tagccaacca ttcgcaccgt tcctctacac
     9421 gctggcgaat gacggtgcat ccattatcaa tccggcggtg ttaaaggaac atgcagcgga
     9481 tgatgctcgc ggcttcctcg cgcaaaatac cgccggttcc ggaccattta tgctgaaaag
     9541 ctggcaaaaa ggtcagcaat tagttctggt gccaaatccg cattactccg gcaataaacc
     9601 gaactttaag cgagtatcgg taaaaattat cggtgaaagt gcctcccgtc gcctgcagct
     9661 ctcccgtggc gacattgaca ttgccgatgc gctgccggtg gatcaactca acgccctgaa
     9721 gcaggaaaat aaagtcaatg tggcagagta tccgtcactg cgcgtcacct atctgtatct
     9781 gaataacagc aaagcgccac ttaatcaggc ggatctgcgt cgggccattt cctggtctac
     9841 cgattatcag ggcatggtta acggcattct gagtggtaac ggaaaacaaa tgcgcggccc
     9901 gattccggaa ggcatgtggg gctacgatgc gacggcaatg caatacaacc atgacgaaac
     9961 gaaagccaaa gccgaatggg ataaagtgac gagcaaaccc accagcctga cgtttctcta
    10021 ctccgataac gatccgaact gggaaccgat cgctctggcg acacaatcca gtctcaacaa
    10081 gctgggcatc aatgtgaagc tggaaaagct ggcgaacgcc accatgcgcg acagagtggg
    10141 taaaggtgat tacgacattg cgattggcaa ctggagtccg gattttgccg acccgtatat
    10201 gtttatgaat tactggtttg agtcagacaa aaaaggtctg ccgggtaacc gctcgttcta
    10261 tgaaaacagt gaggtcgata agttactgcg caatgcgctt gcgaccaccg accagacgca
    10321 gcgtacccgg gactaccagc aggcacagaa aatcgtcatt gatgacgctg cttatgtgta
    10381 cctgttccag aaaaactacc aactggcgat gaacaaagag gtgaaaggct ttgtgttcaa
    10441 tcccatgctg gaacaggtct tcaatatcaa taccatgagt aaataacgtg accttctgga
    10501 gtattttacg ccaacgctgc tgggggctgg tgctcgtggt ggcgggcgtc tgcgtgatta
    10561 cgtttattat ctcgcatctg atccctggtg atccggcgcg gttactggcg ggtgaccgcg
    10621 ccagcgatgc tatcgtggaa aatattcgcc agcatctggg actggaccag ccactgtacg
    10681 tacagtttta ccgctacgtc agcgacctgt ttcatggtga cctcggaaca tccattcgta
    10741 ccgggcgtcc ggtgctggaa gagttgcgta tatttttctc ggcgacgctg gaactggctt
    10801 tttgtgccct gctgctggca ctcctgattg gcattccgct gggcatactc tctgccgtct
    10861 ggcgaaatcg ctggctggat catctggtgc gaataatggc cattaccgga atctccacac
    10921 ctgcgttctg gcttgggctg ggcgtcattg tgctgtttta tggtcatctg caaattcttc
    10981 ccggcggcgg aaggcttgat gactggctgg atccgccaac gcacgttacc ggcttttatc
    11041 tgctcgatgc gctgcttgaa ggcaacggtg aagtcttctt caatgcgttg caacatctca
    11101 tcttaccggc attaacgctg gcgtttgttc acctggggat tgtcgcccgc cagatccgtt
    11161 cagcgatgct ggaacaattg agtgaagact acattcgtac cgccagggcc agcggcttgt
    11221 ccggctggta tatcgtttta tgttatgcgc tacccaatgc gttgatccca tcgattaccg
    11281 tattgggtct ggcgctgggc gatttgttgt atggcgcagt gctcaccgaa accgtttttg
    11341 cctggcccgg aatgggtgca tgggtagtga catcaataca ggcgctcgac ttcccggctg
    11401 tgatgggctt tgccgtcgtg gtttcatttg cttatgtgct ggtcaacctg gtggtggatt
    11461 tgctctattt gtggattgat ccgcgtatcg gacgtggagg tggtgaatga tgctaagcga
    11521 ggaaacgtcc gccgtacgtc cacaaaaaca aacgcgattt aatggtgcaa aactggtatg
    11581 gatgcttaaa ggcagtccgc tcaccgtgac cggcgcagtc atcattgtat taatgctact
    11641 gatgatgatt ttttcaccgt ggctggcgac gcatgatccc aacgccattg atttaaccgc
    11701 ccgccttttg ccgccttctg cggcgcactg gtttggcacc gatgaagtgg gacgcgatct
    11761 gttcagccgc gtactggtcg gcagtcagca atcaattctc gccggattag tggtggtcgc
    11821 cattgcgggt atgattggtt cgctactcgg atgtctatcc ggtgtgcttg gcggacgcgc
    11881 agacgccatc atcatgcgca tcatggacat tatgctgtcg attccttcgt tggtactgac
    11941 aatggcactg gctgccgctc tcgggccgag tttgtttaac gccatgctgg ctattgctat
    12001 tgtgcgaatt cccttttatg tgcgcctggc gcgggggcaa gcattagttg tacgccagta
    12061 tacctatgtt caggcggcga aaacctttgg cgcgtctcgt tggcatctga tcaactggca
    12121 tattttacgt aactccctac cgccgctgat cgtgcaggca tcgctggata tcggtagcgc
    12181 gattttaatg gccgccacgt tgggatttat tggcctgggt gctcaacaac cgagtgctga
    12241 atggggcgcg atggtagcaa atggtcgcaa ctatgtactc gatcagtggt ggtattgcgc
    12301 atttccgggg gcagcgattt tgcttaccgc cgtcgggttt aatctctttg gtgatggtat
    12361 tcgcgatctg cttgacccga aagcaggagg aaagcagtca tgacccaacc cgttctggac
    12421 attcaacaac tgcatttgag tttccccggt tttcacggtg atgttcacgc gctcaacaat
    12481 gtgtccttgc agattaaccg cggtgaaatt gtcggtctgg tgggagaatc cggctcaggt
    12541 aaatcagtca ccgcaatgct gattatgcgt ctgctaccga cgggcagtta ttgcgtacat
    12601 cgggggcaga tttccctgct gggagaagat gttcttaacg cgcgggaaaa acagcttcgt
    12661 cagtggcgcg gcgcacgagt ggcgatgatc tttcaggaac cgatgaccgc cctcaatccg
    12721 acacgtcgaa taggtcttca gatgatggac gtgatccgcc atcatcaacc aataagtcgt
    12781 cgggaagcca gagctaaagc gattgacctg ctggaagaga tgcaaatccc ggatgccgtg
    12841 gaagttatgt cgcgctatcc gtttgagctt tcaggtggta tgcgccagcg ggtaatgatt
    12901 gcgctggcat tctcctgcga gccgcaattg attattgccg acgaaccgac tacggcgctg
    12961 gacgtcacgg tgcagttgca ggtactgcgt ttgcttaaac ataaagcccg cgccagtgga
    13021 actgcggttc tgttcatcag ccatgatatg gccgtggtgt cgcaactgtg cgatagcgtt
    13081 tacgtgatgt atgccggaag cgtaattgaa agcggcgtga cggcagacgt tatccatcat
    13141 ccccggcatc cgtataccat tggtttgctg caatgcgcac cagaacatgg agtaccacgc
    13201 cagctattac ccgccattcc agggacggta ccaaacctca cccatttgcc tgacggctgc
    13261 gcttttcgcg atcgttgcta tgcggcaggt gcacagtgtg aaaacgtccc ggcgctgaca
    13321 gcgtgtggtg acaacaacca gcgctgcgcc tgttggtatc cgcagcagga ggtcataagt
    13381 gtctgacacg ttattaacgt tacgcgacgt ccatatcaat ttcccggccc gtaaaaactg
    13441 gcttggtaaa actacggaac atgttcatgc cattaatggt attgatttac agatccgccg
    13501 tggtgaaacc ttagggatcg tcggcgagtc aggctgcggc aaaagcaccc tcgcacagct
    13561 tttaatgggt atgctgcaac cgagccacgg gcagtacatc cgttctggct cacaacgcat
    13621 tatgcagatg gtgtttcagg acccgctctc ttcgcttaat ccgcgcttac cggtgtggcg
    13681 cgtcatcaca gaaccgctct ggatagctaa gcgtagtagt gaacaacagc ggcgagcgtt
    13741 ggcagaggag ttggctgtgc aggtgggtat tcgtccggag tatctcgacc gcctgcctca
    13801 tgcgttctcc ggcgggcagc ggcaacgtat cgccattgcc agagcactct cttcgcagcc
    13861 tgacgtgatt gtgcttgatg agccaacctc tgcgctggat atctccgttc aggcgcagat
    13921 cctcaattta ctggtaacgc tacaggaaaa tcacgggctg acctatgtgc tgatttcaca
    13981 caatgtctcg gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat
    14041 tgtagaactg ggcgacgcgc agcaggtgct gacggcacct gcacatccat acacccgatt
    14101 attgctggat tccctccccg ccattgataa accgctggag gaagaatggg cattacgtaa
    14161 aacggatctg ccgggaaacc gcacgttgcc gcaaggctgt tttttctacg aacgttgccc
    14221 gctggcaacc cacggatgtg aagtccggca atcattagca ataagggagg acggacgtga
    14281 gctccggtgc tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg
    14341 acgcgtcatc cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc
    14401 agtacctgag agaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag
    14461 gcatcaatac ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaaccg
    14521 gcatccactt tatccagcga cacatcggcg gtggtatcaa tcgatgttgg cgtgaatccc
    14581 gcttccccca gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc
    14641 agttcttcag ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt
    14701 tggttcagca cgccactctc ggtggatact gttcccttcc cgcgtttgat atcgccttcc
    14761 cagtgtgcct gacctttctt atggattgtc attgttgctc tcctgtggg
//
LOCUS       DAFNBJ010000117        14788 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000117 DAFNBJ010000000
VERSION     DAFNBJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.117"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..955)
                     /gene="waaO"
                     /locus_tag="L2X49_003444"
                     /gene_synonym="rfaI"
     CDS             complement(<1..955)
                     /gene="waaO"
                     /locus_tag="L2X49_003444"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBN4798474.1"
                     /translation="MSAHYFNPQEMITKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKVLYLDADIACQGNLKPLITMDLANNIAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSARPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQ"
     gene            complement(971..1768)
                     /gene="rfaP"
                     /locus_tag="L2X49_003445"
     CDS             complement(971..1768)
                     /gene="rfaP"
                     /locus_tag="L2X49_003445"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBN4798475.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(1761..2885)
                     /locus_tag="L2X49_003446"
     CDS             complement(1761..2885)
                     /locus_tag="L2X49_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBN4798476.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(2882..3940)
                     /gene="rfaQ"
                     /locus_tag="L2X49_003447"
     CDS             complement(2882..3940)
                     /gene="rfaQ"
                     /locus_tag="L2X49_003447"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBN4798477.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            4353..5630
                     /gene="waaA"
                     /locus_tag="L2X49_003448"
     CDS             4353..5630
                     /gene="waaA"
                     /locus_tag="L2X49_003448"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBN4798478.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            5638..6117
                     /gene="coaD"
                     /locus_tag="L2X49_003449"
     CDS             5638..6117
                     /gene="coaD"
                     /locus_tag="L2X49_003449"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBN4798479.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(6156..6965)
                     /gene="mutM"
                     /locus_tag="L2X49_003450"
     CDS             complement(6156..6965)
                     /gene="mutM"
                     /locus_tag="L2X49_003450"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBN4798480.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(7063..7230)
                     /gene="rpmG"
                     /locus_tag="L2X49_003451"
     CDS             complement(7063..7230)
                     /gene="rpmG"
                     /locus_tag="L2X49_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBN4798481.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(7251..7487)
                     /gene="rpmB"
                     /locus_tag="L2X49_003452"
     CDS             complement(7251..7487)
                     /gene="rpmB"
                     /locus_tag="L2X49_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBN4798482.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(7704..8372)
                     /gene="radC"
                     /locus_tag="L2X49_003453"
     CDS             complement(7704..8372)
                     /gene="radC"
                     /locus_tag="L2X49_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBN4798483.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            8544..9764
                     /gene="coaBC"
                     /locus_tag="L2X49_003454"
     CDS             8544..9764
                     /gene="coaBC"
                     /locus_tag="L2X49_003454"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBN4798484.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPARVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDEFTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            9742..10200
                     /gene="dut"
                     /locus_tag="L2X49_003455"
     CDS             9742..10200
                     /gene="dut"
                     /locus_tag="L2X49_003455"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBN4798485.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            10307..10903
                     /gene="slmA"
                     /locus_tag="L2X49_003456"
     CDS             10307..10903
                     /gene="slmA"
                     /locus_tag="L2X49_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBN4798486.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(10949..11908)
                     /locus_tag="L2X49_003457"
     CDS             complement(10949..11908)
                     /locus_tag="L2X49_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBN4798487.1"
                     /translation="MDATRTTLLALDLFGSPGWSADKEIQRLHALSNHAGRHYRRIIL
                     SKRHGGQRLVLAPDYLLKTVQRNILKNVLSQFPLSPFATAYRPGCPIVSNAQPHCQQP
                     QILKLDIENFFDSISWLQVWRVFRQAQLPRNVVTMLTWICCYNDALPQGAPTSPAISN
                     LVMRRFDERIGEWCQARGITYTRYCDDMTFSGHFNARQVKNKVCGLLAELGLSLNKRK
                     GCLIAACKRQQVTGIVVNHKPQLAREARRALRQEVHLCQKYGVISHLSHRGELDPSGD
                     LHAQATAYLYALQGRINWLLQINPEDEAFQQARESVKRMLVAW"
     gene            complement(12206..12847)
                     /gene="pyrE"
                     /locus_tag="L2X49_003458"
     CDS             complement(12206..12847)
                     /gene="pyrE"
                     /locus_tag="L2X49_003458"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBN4798488.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(12914..13630)
                     /gene="rph"
                     /locus_tag="L2X49_003459"
     CDS             complement(12914..13630)
                     /gene="rph"
                     /locus_tag="L2X49_003459"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBN4798489.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            13757..14620
                     /locus_tag="L2X49_003460"
     CDS             13757..14620
                     /locus_tag="L2X49_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBN4798490.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
BASE COUNT         3701 a         3876 c         3561 g         3650 t
ORIGIN      
        1 tttgtttaaa attatgcttg gcgcaataac gagcatagtt tgagttaact ggccgcatta
       61 acggttcatt tttccagggc gatgcctctt tggctttgat aaaaggtcgc gcacttggat
      121 aaccggccca gtaatgccag ggttttgtcg ggccgacata atgaattaat acggtttcat
      181 cattaattgg acaaacaaat gattttttta attcataatt taaactaaat tgcgtattgt
      241 atttagcatc aatgaattta actttccctg acaggataag attaagtata tcttgatcca
      301 tataggttaa acgggaaacg acggctttat cagcaagcat cgacatcgct ttagcagaaa
      361 cggactcctg cgcccacgct agtgtattaa ttaacaggac acctgaatta aagtaaccct
      421 tttcaagttc attacactgc agactttgac cccgtaacga ccaccagtta gcatcgcgtt
      481 cagtaacaac agcagcaatg ttattggcaa gatccattgt tatcagcggc tttaagtttc
      541 cctgacaggc gatatcagca tccaggtaaa gaaccttatc ttgttgatca ataaagtaat
      601 ctgcaattac aaaacggaaa tacatggcaa tagaccaatt tttggtcgtc ggtaatgcct
      661 taagccgttc acaatttacc agatggatct ggatacaggt acgatagctt ttcgccaatt
      721 gggctaaacg ctggatatcg gcttcaggga tatcatcaat aaaaacgtgg aaaacaaaac
      781 tcacgtcgtt gttatgtaac aaaactgacg tgattgaaac accacaacca aaaagaaagt
      841 ttttatcaat gccataagca acatggaatg atgatgccac tgacgccgct ggcctttcat
      901 cgaagatgat tgtcttagtg atcatctctt gtggattaaa atagtgggca ctcatatttc
      961 tctccggaaa ttacaacgat tttcgaatcg ttctttccct gatttttgtg gcttttgctt
     1021 ctgcttgcga cagcagtcct tgttcctgct tgagaatgtc tttaagcggg gcggcaaaat
     1081 acactttcat aaaccgccag atatcccgct gagtcaggcc gatattcatc gaagaaaaat
     1141 aaagcccaat aagatcttta tcccgccaac gacgtggaac gcgcgtgcga agctgcgccc
     1201 ggtgcaggtc aattaccgaa atttttaact cctcttcctt accggaaaaa ggcaagtgca
     1261 gcaggaaatg acagatataa cagtcacggt ggttaatgcc cgcagcatgc atatcgcgca
     1321 ccatcgtcgc gacacgctta ataagcatac gctttacgcg aacatctgga gggttagtcg
     1381 cccagtcagc acagtaatct tccagactta tggttggtgt cagatcttcg gtaataataa
     1441 acgaggttct ggtcagcgga ttcatgcctt tttcgccgaa tgccacccca tacatagtat
     1501 caacgccgac atcccgcagt cgatgaatcg cattccattc gcggtctgcg cctaatactg
     1561 gcatccgcaa tgagagtaaa tttttgatta tctctttcag ggtcgtgccg cgatgccatt
     1621 tgagaaaata gcttttgccc gccatttcaa agcgcagagt acggcgagtt tccagttcac
     1681 gaaatacctc accctgcaag gttttaactt cctcaaaagg atctttgccg cgccataacg
     1741 tggcaaacgg ctctttaagt tcaaccatcc agaccacccg ttatgatatc cgccgctttc
     1801 tctggcagac tgtataaatc ttgtgtatca gcataatgtc gcgcattttc cgcccaggcc
     1861 tggcgcaatg aagattgcgt taacgctttg cgtaaaatct cattcaatgt ttcctggcgg
     1921 aatggctcag caatagcctc gccgcaattg gcgtcgacaa tataatgcgc atagccacaa
     1981 acggctgttg ttagtaccgg taatcctgca gtaatcgctt ccagcagcac aattcccgcc
     2041 gcttcctggt aggcaggatg cagtaataaa tccgccgccg ccattaattc cgagacatca
     2101 ttgcgccccg agaaaaagtg aacattactg cgcacgccgc gtttttctgc cagcgcctca
     2161 aattttcgcg gtttatcctg cccaacaaca tatagcaatg tgttgtggcg cagcgaatcc
     2221 ggtaacgaag caagtgcttc aatggaacga tcgacacctt tacgcgtgaa gtctgaaccg
     2281 acctgcaaca ataaatattg ttgttcggtt attccattct tcttacggaa gatttcacgg
     2341 ctattggctg gctgctggct atatttacga tcaggataaa tccccggtgg cagaatatga
     2401 aaacgctccg cttcagtctg ataatgtttc tggaaatcgg cgatttgctt atctgtcagc
     2461 atcagcaact gtgtcggctt accctgttcg aaggttgccc gctcaaaggc ggcataatgg
     2521 cgataacgtg acgtcaggcg atagaaaaag cctttttcct gcgctacttt ctcggcataa
     2581 caaacatcag cggcataata aacgtccagc cccggcattt tgttgaatcc aacgacttta
     2641 tcgacgggat gttcgcgtaa atgtttttgc acccaggcaa aatactccgc attgcgcccg
     2701 tgattggtat gcgatttaac cggcactttg atcagttcaa atacatcagg gcattcgcct
     2761 tcccacgact gggtataaac ccgaacatga tgacctcggg cggcgactgt ctgagcaata
     2821 cgcataaaat cacgctgcaa accgccaaag ggaaaatatt tatataaaca aaaagcaacg
     2881 atcataacga cgtacctgtc gtggaggagg gcagtaattt atcgacagca gcaatgacat
     2941 ctgccgccgg aataaccgaa agatacatct catttcggtc acgctgatcg cgcgttggca
     3001 tttcccggta atctcccgcc cagaattgaa tcatgttatt tgaccaggga cgccagaaaa
     3061 tatggtctgt cgcaccaaac agcgatatca gcggcgtatt aactgcagcg gcaatatgcg
     3121 ccggtgcgga atcaacgcca ataaacagct gcgcatgatc gattaacgca ccaagttccg
     3181 ggaaggtcac ctttccagcc agcgccgtta ctggtggcgt ctggcatccc tgcgcaattt
     3241 cattgacgca ggccagatcg tctttatccg ggccggacgt cagaacgact tcataaccac
     3301 gagcatgtaa ggcatcaatc acagcggaaa acttggcgtt gtcccagcat ttgaagattt
     3361 gccgcgccgt aggttggata accacataat tttgtccaac accagcgtga tcaagttcgc
     3421 gacgcatacg tttccagctt gcaggcgggt aactcatggt tgtctgcttc accaacgaat
     3481 caagtcccaa tggtgtcagc acggataagt tactttccac cacatttcca ccctgcaacg
     3541 gcaccaaatg ggtgaaactt ttacgccaaa aagcagactg ccgatgatga taatcctggg
     3601 aaattttcac acgggcattt aataagcgaa ccagtatagc aaccatccat tgatcggtaa
     3661 gattgacgat aaggtcatac ttattggcac gtaatacctt gatgagatga aaaaagttgg
     3721 caattttttc tgaggctttt gcttttttat tttttatgcc gtagagcgcg ttaatctctg
     3781 gattttcaga caggatcggg atggtgtcct gataaagcag cacatcgatt tttgcgtcag
     3841 ggtaattttt tttcagcgaa ctaatgacgg gagtagttaa taacatatcc ccatgaaaac
     3901 gcatcttaat gagcaaaatt cttcgaaatg gcttatccac aagtaactct ttttgttgag
     3961 attatccgat taaggtgaag cagaaaaagc acgctaccgc cccaggctca acagctacct
     4021 gaatactgat accgcactga tattgacgcg ctagtgtatc acttctttcg cggcaatccg
     4081 acccgaatga tttttaagca cattgttgat tttcaataaa gaaatcaatc aaataaaatc
     4141 attagcaacc atattctttc actgttgctc tgcgattcct tcctgcacgt acagcagaaa
     4201 tcaatgaatc gcataaaaat acaaaaaaag tatagatgtt cagcaagaat tggcgggtaa
     4261 attgctattt aagtcaaaaa cgggaaaagt aatggtaaag ccacagctaa atacatagaa
     4321 tccccagcac atccataagt cagctattta ctatgctcga attgctttac accgcccttc
     4381 tctaccttat tcagccgctg atctggatac ggctctgggt gcgcggacgt aaggctccgg
     4441 cctatcgaaa acgctggggt gaacgttacg gtttttaccg ccatccgcta aaaccaggcg
     4501 gcattatgct gcactccgtc tccgtcggtg aaactctggc ggcgatcccg ttggtgcgcg
     4561 cgctgcgcca tcgttatcct gatttaccga ttaccgttac aaccatgacg ccaaccggtt
     4621 cggagcgcgt acaatcggct ttcgggaagg atgttcagca cgtttatctg ccgtatgacc
     4681 tgcccgatgc gctcaaccgt ttcctgaata aagtcgaccc caaactggtg ttgattatgg
     4741 aaaccgaact atggcctaac ctgattgcgg cgctacacaa acgtaaaatt ccgctggtga
     4801 tcgctaacgc gcgactctct gcccgctcgg ccgcaggtta tgccaaactg ggtaaattcg
     4861 tccgtcgctt gctgcgtcgt attacgctga ttgctgcgca aaatgaagaa gatggtgcac
     4921 gttttgtggc gctgggcgca aaaaataatc aggtgaccgt taccggtagc ctgaaattcg
     4981 atatttctgt aacgccgcag ttggctgcta aagccgtgac gctgcgccgc cagtgggcac
     5041 cacaccgccc ggtatggatt gccaccagca ctcacgaagg cgaagagagt gtggtgatcg
     5101 ccgcacatca ggcattgtta cagcaattcc cgaatttatt gctcatcctg gtaccccgtc
     5161 atccagaacg cttcccggat gcgattaatc ttgtccgcca ggctggacta agctatatca
     5221 cacgctcttc aggggaagtc ccctccacca gcacgcaggt tgtggttggc gatacgatgg
     5281 gcgagttgat gttactgtac ggcattgccg atctcgcctt tgttggcggt tcactggttg
     5341 aacgtggtgg gcataatccg ctggaagctg ccgcacacgc tattccggta ttgatggggc
     5401 cgcatacttt taactttaaa gacatttgcg cgcggctgga gcaggcaagc gggctgatta
     5461 ccgttaccga tgccactacg cttgcaaaag aggtgtcctc tttactcacc gacgccgatt
     5521 accgtagttt ctatggccgt catgccgttg aagtactgta tcaaaaccag ggcgcgctac
     5581 agcgtctgct tcaactgctg gaaccttacc tgccaccgaa aacgcattga ggttgttatg
     5641 caaaaacggg cgatttatcc tggtactttc gatcccatta ccaatggtca tatcgatatc
     5701 gtgacgcgcg ccacgcaaat gttcgatcac gttattctgg cgattgccgc cagccccagt
     5761 aaaaaaccga tgtttaccct ggaagagcgt gtggcgctgg cacagcaggc aaccgcgcat
     5821 ctggggaacg tggaagtggt cgggtttagt gatttgatgg cgaacttcgc ccgtaatcaa
     5881 cacgctacgg tgctgattcg tggcctgcgt gcggtggcgg attttgaata tgaaatgcag
     5941 ctggcgcata tgaatcgcca cttaatgccg gaactggaaa gtgtgtttct gatgccgtcg
     6001 aaagagtggt cgtttatctc ttcatcgttg gtgaaagagg tggcgcgcca tcagggcgat
     6061 gtcacccatt tcctgccgga gaatgtccat caggcgctga tggcgaagtt agcgtagcgt
     6121 ttatgccgga tggtatgcca tccggcgcgc atgaattact tctggcactg ccgacaataa
     6181 aacgttgccc gctgcgcatg tttagtcgcc acaatcggcg taccgcacac ccgacacggc
     6241 tcgccttttc gcccgtaaac ctgcaattcc tgcgcgaaat agcccggttt accatcactt
     6301 tgcagaaaat ctttcagcgt tgtaccaccc tgctcaatcg aacgcagcaa caccgcttta
     6361 atcacccgag ctaacaattc acactctgcc agcgacagtg atgacgccag ccgatccgga
     6421 tggatccccg ccgcaaacag tgattcgctg gcatagatat tccctacccc taccaccagc
     6481 ttgttatcca tcagccacgg tttaatcgcc gtttttttct tcgcgcactt ctgatgcaga
     6541 tactcaccat tgaaatcgtc gctgagcggc tccggtccaa gatgggccag cacattatgc
     6601 ccttccagct ctttggtcca cagccaggca ccaaagcggc gcggatcggt gtagcgcagc
     6661 actttaccgt tgctcatcac caaatccaca tggtcatgct tttcaggggg aagttcttct
     6721 ggaaggatgc gcaggctgcc agacatcccc aaatgaatga taatccagcc ctcaggcagc
     6781 tccagcagca aatatttagc ccgccgctgc acgctaagca ctggttggtc gcttaaacgg
     6841 tagatctctt ctgaaaccgg ccagcgcaag cgtccgttgc gcaccactgc atgaagaatg
     6901 gttgcaccaa cgagatgcgg ttctatgccg cgacggctgg tttcaacttc gggtaattca
     6961 ggcatagcat ctccaggaat gaacagatgc aatcaatatg ggggcaagca gataacaaaa
     7021 aaccccgccg gagcgaggtt ttttgttaca tcaaagcgag aattatttga ttttcgcttc
     7081 tttgtagatc acgtgctgac gaacaactgg atcgaatttt ttcagttcca gtttttccgg
     7141 cttagtacgt ttgttcttcg tagtggtata gaagtgacca gtaccagcag aagaaaccag
     7201 cttgattttc tcacgaatac ctttagccat gatttatttc ctctaagtac ttagtacttt
     7261 tcgccacggg cacgcagttc agccagaact gtatcgatgc cttttttatc gattacacgc
     7321 atacctttag cagatacgcg cagggtgaca aaacgcttct cgctctcaac ccagaaacgg
     7381 tgagagtgca ggttcggcag gaaacggcgt ttagtcgcgt tcagtgcgtg ggaacggttg
     7441 ttaccggtca ccggacgctt gccagtaact tggcagactc gggacatgtc tattctccaa
     7501 aaatcaaatt agctcgagct tcgtatgggg tattggcgcc tcgtcaggct ttacagcccg
     7561 gtcatcgcag ttctatgtga actctcgatt gccaggccca aatgccaaac ccgagattct
     7621 caaaggtggc gtagtatacg ctgactcagc gatgtgctca agtcccgaac agacaaagat
     7681 cccgaaggat cgcgcatagc gggttaaatc cagccgcgtt cggcaaaaga aacatactct
     7741 ccacgcccaa tcacgatatg gtcgagcacg cgtaaatcca tgaactgaca actctttatt
     7801 atccgttcag taatgagttt atccgcttta ctgggttcag cacaacccga agggtgatta
     7861 tgtgcaagga tcagcgccga ggcgtttatt tttatcgctt cgcggataat ttcccgagga
     7921 tggacttcaa catggtttag cgtgccggaa aaaagtcggc tatgcgttat aacccggtgt
     7981 tgtgagtcga gaaagatcac cataaagatc tcccgctcct cacccgtgag ctggctttgt
     8041 aaaaattccc gcgtcatctc cgggctgagt aaagggcttt cttcacgcat ccgcacgttg
     8101 tagtaacgcc gcgccagttc agcaatcccc tttaactggg caaatttcgc tacgccaatt
     8161 ccatgaacac cactaaattg ttcatattca gaggttaaca agccataaag agagccgaaa
     8221 ttctccaaca tctcttttgc cagggttaat acatctttac cgcgcgttcc ggtacgcaga
     8281 aatagcgcca gcagctcgac atccgttaag gcgctaatac caaacttcag cattttttcg
     8341 cgcggcatca acagctgtgc attgtttttc accttcacct cctttgtggt gctcgcatcc
     8401 tgtcatagcc gttccgttaa atcgacggcc agttttcaat cctggaaagc gcctcgcaaa
     8461 gtgaatctca ggtgaaggca cgacaacaaa agtattgtga taaaatcgcc aacttctggt
     8521 gtcacccaac aggaaaaatc atcatgagcc tggccggtaa aaaaatcgtt ctcggcgtta
     8581 gcggcggtat tgctgcctat aaaacccctg aactggtgcg tcgtttgcgc gatcgcgggg
     8641 ccgacgtccg cgtagccatg accgaagcgg caaaagcctt tatcacccca cttagtttgc
     8701 aggcggtttc tggttatccc gtttccgaca gtctgctgga cccggcagcc gaagccgcta
     8761 tgggccatat tgagctgggt aaatgggctg atttagtgat tctcgcccct gccacggcag
     8821 atttgattgc ccgtgttgct gccggaatgg caaatgatct ggtatcgacg atttgtctgg
     8881 ctacacctgc gcgtgtagcc gtgctccccg ccatgaacca gcagatgtac cgtgccgctg
     8941 ccacgcagca taatttagag gtgcttgcct cccgtggttt gctcatctgg gggccagaca
     9001 gtggcagtca ggcttgtggt gatatcggcc ctgggcgaat gctcgatccg ttaaccattg
     9061 tggatatggc ggtagcgcat ttttcgcccg tcaacgacct gaaacatctg aacattatga
     9121 ttaccgccgg tccgacgcgt gaaccgctcg atccggtgcg ttatatctct aatcacagct
     9181 ccggcaagat gggttttgct atcgctgccg ccgctgcccg tcgtggcgcg aacgtcacgc
     9241 tggtatcagg tccagtttca ctaccgacac caccgtttgt taaacgtgtt gatgtgatga
     9301 ccgcgctgga aatggaagcc gccgtgaatg cttctgtaca gcagcaaaat atttttatcg
     9361 gttgcgccgc cgtggcggat tatcgcgcag ctaccgtggc cccagaaaaa atcaaaaagc
     9421 aggccacgca gggtgatgaa ttcacaataa aaatggttaa aaaccccgat atcgtcgcag
     9481 gcgttgccgc actaaaagac catcgaccct acgtcgttgg atttgccgcc gaaacaaata
     9541 atgtggaaga atacgcccgg caaaaacgta tccgtaaaaa ccttgatctg atctgcgcga
     9601 acgatgtttc ccagccaact caaggattta acagcgacaa caacgcatta caccttttct
     9661 ggcaggacgg agataaagtc ttaccgcttg agcgcaaaga gctccttggc caattattac
     9721 tcgacgagat cgtgacccgt tatgatgaaa aaaatcgacg ttaagattct ggacccgcgc
     9781 gttgggaagg aatttccgct cccgacttat gccacctctg gctctgccgg acttgacctg
     9841 cgtgcctgtc tcgacgacgc cgtagaactg gctccgggtg atactacgct ggttccgacc
     9901 gggctggcga ttcatattgc cgatccttca ctggcggcaa tgatgctgcc gcgctccgga
     9961 ttgggacata agcacggtat cgtgcttggt aacctggtag gattgatcga ttccgactat
    10021 cagggccagt tgatgatttc cgtgtggaac cgtggtcagg acagcttcac cattcaacct
    10081 ggcgaacgca tcgcccagat gatttttgtt ccggtagtac aggctgaatt taatctggtg
    10141 gaagatttcg acgccaccga ccgcggtgaa ggcggctttg gtcactctgg tcgtcagtaa
    10201 cacatacgca tccgaataac gtcataacat agccgcaaac atttcgtttg cggtcatagc
    10261 gtgggtgccg cctggcaagt gcttattttc aggggtattt tgtaacatgg cagaaaaaca
    10321 aactgcgaaa aggaaccgtc gcgaggaaat acttcagtct ctggcgctga tgctggaatc
    10381 cagcgatgga agccaacgta tcacgacggc aaaactggct gcctctgtcg gcgtttccga
    10441 agcggcacta tatcgccact tccccagtaa gacccgcatg ttcgatagcc tgattgagtt
    10501 tatcgaagat agcctgatta ctcgcatcaa cctgattctg aaagatgaga aagacaccac
    10561 agcgcgcctg cgtctgattg tgttgctgct tctcggtttt ggtgagcgta atcctggcct
    10621 gacccgcatc cttaccgggc atgcgctaat gtttgaacag gatcgcctgc aagggcgcat
    10681 caaccagctg ttcgagcgta ttgaagcgca gctgcgtcag gtattgcgtg aaaagagaat
    10741 gcgtgagggt gaaggttaca ccaccgatga aaccctgctg gcaagccaga tcctggcctt
    10801 ctgtgaaggt atgctgtcac gttttgtccg cagcgagttt aaataccgcc cgacggatga
    10861 ttttgacgcc cgctggccgc taattgccgc gcagttgcag taattctccc ctaaacggtc
    10921 aggcagaaac gtctgcctga cgcttttctt accatgcaac cagcattcgc tttacactct
    10981 ctctcgcctg ttgaaaggcc tcatcctcag ggttgatttg caataaccag tttattcttc
    11041 cctgcaaagc ataaagatac gccgttgcct gtgcgtggag atcgccagaa ggatcaagtt
    11101 caccacgatg actaagatgc gaaataacgc catatttttg gcacaaatgc acctcctgac
    11161 gcagcgcccg gcgcgcttca cgggcaagct gtggcttgtg attaacaaca atcccggtta
    11221 cttgctggcg cttacaggca gctatcaggc agcctttgcg tttattgagg ctcaggccca
    11281 gctccgctaa caatccgcac actttatttt taacctggcg ggcattgaag tgacctgaaa
    11341 aggtcatgtc atcgcagtag cgggtgtagg taattccccg agcctgacac cattccccta
    11401 tgcgttcatc aaaacggcgc atcacaagat tggaaatggc tggcgaagtt ggtgccccct
    11461 gcggtaacgc gtcgttataa caacaaatcc aggtcagcat ggttaccaca ttacgtggca
    11521 actgggcctg gcgaaacaca cgccagacct gtaaccagct aatgctatcg aaaaagtttt
    11581 cgatatcgag tttcaggatc tgcggctgtt ggcagtgtgg ctgcgcgttg ctgacgattg
    11641 ggcaacctgg tcggtaggct gtagcaaaag gggaaagcgg aaattgtgaa aggacgttct
    11701 taagaatgtt gcgctgtacg gttttgagca agtaatcagg ggctaacacc agccgctgac
    11761 caccgtggcg tttagaaaga ataatgcgtc ggtaatggcg tccggcatga ttactgagcg
    11821 catgcagtcg ctgtatttct ttatcggcgc tccagcccgg cgagccgaac aaatcgagcg
    11881 ccagaagggt tgtccgggta gcatccattc cactctccac atcacggcgg ccaaaagagg
    11941 cgccaacagc ggcaatacca ttaaaccatc ccgaaggcgc gtaactgtac tgagcgcgtc
    12001 agcgcgacgt acgcgaagcg tactcaggta caatgaagcg actttgggta tatggacata
    12061 ctactggcgt taagtcagcg actcgccgac cctcccggca caaaaggccg gaaacgctat
    12121 tgccactgct ggcgaaagca gattaacacg atatcttgaa gcgcatcacg ctgcgcacag
    12181 caaaaaccct gcacagcgca tatttttaaa cgccgaactc ttcgcgatag gccttaaccg
    12241 ccgccagatg ttccgccatt tccggcttct cttccaggta agcaatcagg tctttcaggg
    12301 tgatgataga gatcactttg cagttgtaat cacgctcaac ttcctgaatc gccgaaatct
    12361 cgccgcgccc ccgttcctga cgatcgagcg aaatcaacac gccagcaagc gtcgcgccat
    12421 tggcctgaat aatttccatc gactcacgaa tcgccgttcc ggcggtgatc acatcatcta
    12481 ccagcattac gcgtccttgt aacgcgctac caaccagatt gccgccttca ccgtgatctt
    12541 ttgcttcttt gcggttaaag cagtacggca ggtccaggtc atgatgctcc gccagcgcca
    12601 cggcggttgt ggtggcaatc gggatccctt tgtaagcagg gccaaacagc agatcgaact
    12661 caatgccgga atccaccaac gcttcagcgt aaaaacggcc taacagtgcc agatcgcgcc
    12721 cggtattaaa cagcccggcg ttgaagaaat aggggctttt gcgcccggac ttcaacgtaa
    12781 actcgccaaa ctttaacacc tgcttgctaa gcgcaaattc aataaactgg cgctgatatg
    12841 gtttcatgcc ttcgctcctc atcttacttt tctacagaca aaaaaaaagg cgactcatca
    12901 gtcgccttaa aaatcagttt gccagcgccg ccttctgcgt cgctacaatg gattcgattc
    12961 cccctcgggc cagcgccaac aaggtgagta actcttcatg ggtgaacggc tcgccttctg
    13021 ccgtcccctg cacttcaata atgcgcccgt cttcggtcat cactacgttc atgtcggtct
    13081 ctgcggctga gtcttcaacg tattccagat cgcaaattgc ttcaccgttt acaataccga
    13141 cagaaaccgc ggctaccatc cctttcattg gattggtttt cagcttgccg ttttccacca
    13201 gcttctgtag cgcatctgcc agcgccacac aggcacccgt aatcgaagcg gtacgcgtgc
    13261 caccatcagc ctgaagcacg tcgcagtcca gcgtaatggt gaactcaccc agcgctttca
    13321 aatctactgc cgcgcgaagt gcacgggcga tcagacgctg gatttccatt gtgcgtccac
    13381 cctgcttacc tttcgctgct tcacgagcgt tacgggtgtg ggtagaacgc ggcagcatac
    13441 cgtactctgc ggtgatccag ccctggccct gacctttcag gaagcgcggc acgccttctt
    13501 caatagaggc ggtacacaac actttggtat cgccaaattc gaccagcacc gagccttctg
    13561 catgttttgt atagttacga gtcagggtaa cgggacgcac ctgattattg ctacggcctg
    13621 ctggacgcat attgaaatct ccggcttgaa acaaatgtgg ctgcgcatta tacggacttc
    13681 cggcggttat tcctatcctg acaaggcatc gatggctata atcctcccac ctctcctttt
    13741 ataaacagga acgtctatga tccgcagtat gaccgcctac gcccggcgtg aaatcaaggg
    13801 tgaatggggg agcgcaacct gggaaatgcg ctcggtaaac cagcgttatc tggaaactta
    13861 ctttcgtctg ccggagcagt tccgtagcct tgaacctgtc gttcgcgagc gtattcgttc
    13921 tcgcctgacg cgcggtaaag tggaatgtac cctgcgctat gagccagatg ttagcgcaca
    13981 aggtgagctg atcctcaacg aaaaactggc taaacagctg gtaactgccg cgaactgggt
    14041 aaaaatgcag agtgacgaag gggaaatcaa cccggttgat attctacgct ggccgggcgt
    14101 gatggcagcc caggagcagg atctcgacgc gattgccgct gaaattctcg cggcgttgga
    14161 tggtaccctg gacgacttta tcgtcgcgcg cgaaaccgaa ggtcaggcgc tgaaagcatt
    14221 gatcgagcag cgtctggaag gcgtcaccgc cgaagtggtc aaagtccgcg cccatatgcc
    14281 ggaaatcctg caatggcagc gtgagcgtct ggtcgcgaag ctggaagatg ctcaggttca
    14341 gttggaaaac aaccgtctgg agcaggagtt ggttctgctg gcacaacgaa ttgacgttgc
    14401 cgaagaactg gaccgcctgg aagcgcatgt caaagagacc tacaacattc tgaagaaaaa
    14461 agaagccgtt ggtcgtcgtc tggactttat gatgcaggaa ttcaaccgcg agtcgaacac
    14521 tcttgcgtcg aaatctatca atgccgaagt gacaaactcc gccatcgagc tgaaagtgtt
    14581 gattgagcag atgcgcgagc agattcagaa tattgagtaa cttgtaacac taattcttac
    14641 ttttcagcct tcacaaatgc cgcccactca aacagagcgg catttttctt ccccgcaact
    14701 tcaattctgt tttgcgtgcc tgctccagat tttgcgatgt ttttttgccc agcacactga
    14761 gaacgtgaga tactcacaac tgtatata
//
LOCUS       DAFNBJ010000118        14764 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000118 DAFNBJ010000000
VERSION     DAFNBJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.118"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(135..350)
                     /locus_tag="L2X49_003461"
     CDS             complement(135..350)
                     /locus_tag="L2X49_003461"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798491.1"
                     /translation="MPVCPRREKTRAGGAERGRAHAPPLSLRGRSDGGFCFFMPVICL
                     LLMMLLFLLLLLLLLLLLLLFFFFLLL"
     gene            complement(376..939)
                     /locus_tag="L2X49_003462"
     CDS             complement(376..939)
                     /locus_tag="L2X49_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBN4798492.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(987..2348)
                     /locus_tag="L2X49_003463"
     CDS             complement(987..2348)
                     /locus_tag="L2X49_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HBN4798493.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(2400..2630)
                     /locus_tag="L2X49_003464"
     CDS             complement(2400..2630)
                     /locus_tag="L2X49_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798494.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(3665..3856)
                     /locus_tag="L2X49_003465"
     CDS             complement(3665..3856)
                     /locus_tag="L2X49_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798495.1"
                     /translation="MNISTETRGILRNYRAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(3853..4275)
                     /locus_tag="L2X49_003466"
     CDS             complement(3853..4275)
                     /locus_tag="L2X49_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBN4798496.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            complement(4322..4624)
                     /locus_tag="L2X49_003467"
     CDS             complement(4322..4624)
                     /locus_tag="L2X49_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBN4798497.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(5107..5877)
                     /locus_tag="L2X49_003468"
     CDS             complement(5107..5877)
                     /locus_tag="L2X49_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798498.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIYVR
                     LDAPRLATLLINRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLAAVRDAIRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARVEIRSMADALAKIRD"
     gene            complement(5922..6356)
                     /locus_tag="L2X49_003469"
     CDS             complement(5922..6356)
                     /locus_tag="L2X49_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBN4798499.1"
                     /translation="MYGTCETLCRELEAKYPGDTPLMLVIWSPEEIQALADGMEISLT
                     DHEIRAVLARMEDIPEDQRTESGISSAAVMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLVVPECVTRRQAVVNQARALLKNNTREND"
     gene            complement(6369..6590)
                     /locus_tag="L2X49_003470"
     CDS             complement(6369..6590)
                     /locus_tag="L2X49_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798500.1"
                     /translation="MNYAGHENLRAEVAEVANAMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(6591..7274)
                     /locus_tag="L2X49_003471"
     CDS             complement(6591..7274)
                     /locus_tag="L2X49_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBN4798501.1"
                     /translation="MSRFILGDCVRVMATIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWNRIDRFMAAWKRAGFSVVGHLVFTKSYT
                     SKSAYVAYRHECAYILAKGRPALPPKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLATVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(7351..7659)
                     /locus_tag="L2X49_003472"
     CDS             complement(7351..7659)
                     /locus_tag="L2X49_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798502.1"
                     /translation="MVCFGLFLGFIWRVTKDVAVLLFLLRRLTRRGRNAACGQCPASG
                     RARDGRFYRSWLASVTVYASLSPFSDECPSSRFRGISSPSERRRLRYSTVGLTRYRTR
                     "
     gene            7791..8753
                     /gene="parM/stbA"
                     /locus_tag="L2X49_003473"
     CDS             7791..8753
                     /gene="parM/stbA"
                     /locus_tag="L2X49_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBN4798503.1"
                     /translation="MLVFIDDGSTNIKLQWQENDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            8756..9106
                     /locus_tag="L2X49_003474"
     CDS             8756..9106
                     /locus_tag="L2X49_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBN4798504.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCETLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            9215..9677
                     /locus_tag="L2X49_003475"
                     /pseudo
     CDS             9215..9677
                     /locus_tag="L2X49_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9902..10903)
                     /locus_tag="L2X49_003476"
     CDS             complement(9902..10903)
                     /locus_tag="L2X49_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HBN4798505.1"
                     /translation="MTEPLNLRTDVVRQHTVPRFLLKHFSKPVKGKRQRLHAFDKAAG
                     RAYATTPDDATVRNTFYNFDNHPQRFSLEPLLGIYEHDAAPVIAGLLEHKDIRRLTED
                     DRYKLAVFVAVQRARTFGELERISGMISVLTDKLAAMGVTEEQAGETLGLSPGGDTRD
                     IFLRQLVQQVSHIKHLLSKDWYLLETRPEHPFYVSDNPVVLENRNDFGVYGNIGLAVP
                     GIQIYLPLSSTLMLAMYCPSIREQKVREKQHLLHLIARAPDLIPRHMRPFEMLEHVNR
                     HTDYLLMPLSAENVMHYNALQVEYAEQYVFCGENDFSLAERMLAADDRYRTGPRFTF"
     gene            11206..13881
                     /locus_tag="L2X49_003477"
     CDS             11206..13881
                     /locus_tag="L2X49_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798506.1"
                     /translation="MVGSRWYKFDFHNHTPASHDYKIPDISPREWLLAYMKQHVDCVV
                     ISDHNSGAWVDVLKGELENMSRDASTGDLPEFRPLTLFPGVELTATGNVHILAVLHTH
                     STSADVERLLAQCNNNSPIPSEVPNHQLVLQLGPAGIISNIRRNPKAVCILAHIDAAK
                     GVLSLTNQAELTAAFQESPHAVEIRHRVEDITDGTRRRLIDNLPWLRGSDAHHPEQAG
                     MRTCWLKMSSPDFDGLRHALLDPENCVLFDQLPPEEPASYLRSLKFRTRHCHPVGQDS
                     ASVEFSPFYNAVIGSRGSGKSTLIESIRLAMRKTEGLTATQGSKLDQFIRTGMEADSF
                     IECIFHKEGTDFRLSWRPDSKHELHIFSDGEWMPDSHWSADRFPLSIYSQKMLYELAS
                     DTGAFLRVCDESPVVNKRAWKERWDQLEREYLNEQITLRGLRARQGSADSLRGELSDA
                     ERAVSQLQSSAYYPVCRQLALARNELSAATLPLEHFERRIAAIQALAEEPLQRSDIPP
                     EPSGLLMAFMARLSSVQQQYDQRLNTLLAEYAAELAGIRREQSFIALRTAVSDQETNV
                     ESEAVSLRARGLNPDVLNELMARCESLKNELRNYDGLDGAISASVARSEQLLAEMRAH
                     RMALTDNRKAFLSSLSLSALEIKILPLCAPYEDVISGYQTVTGISNFAERIYDNSDGS
                     GLLSDFISERPFSPLPAATEKKYRALDELKALHHSIRLDNSEAGAGLHGSFRNRLRSL
                     NDQQLDALQCWYPDDGIHIRYQTPGGQMEDIAFASPGQKGASMLQFLLSYGTDPLLLD
                     QPEDDLDCLMLSMSVIPAIMSNKKRRQLIIVSHSAPIVVNGDAEYVISMQHDRTGLYP
                     GLCGALQEAPMKALICRQMEGGEKAFRSRYERILS"
     gene            14053..14220
                     /locus_tag="L2X49_003478"
     CDS             14053..14220
                     /locus_tag="L2X49_003478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798507.1"
                     /translation="MFTEKQKIRAIELFCRDKTESRWPASCCLLVTRQASLFKKQACR
                     TIILYIQTDPL"
     gene            complement(14227..14396)
                     /locus_tag="L2X49_003479"
                     /pseudo
     CDS             complement(14227..14396)
                     /locus_tag="L2X49_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
BASE COUNT         3574 a         3963 c         3851 g         3376 t
ORIGIN      
        1 ccagcgggtt cggcggagac tggcaggtgc gccagcagct gcctgtcgga gtcgggcctg
       61 acgaccggat gccgccaggc agccgggagc cgaaggtgcg cccggcagac aggacaacac
      121 tgcagtagtc agaatcagag cagcaggaaa aagaagaaca gtagcagtag cagtagcagt
      181 agcagtagca gtagcaggaa caacaacatc atcagtaaca gacaaatgac aggcatgaag
      241 aaacagaagc ccccatcaga acgtccgcgt aatgaaaggg gcggcgcgtg agcgcggccc
      301 ctttcagcgc caccggcgcg cgttttttcc cggcgcgggc agacaggcat ttatcctccc
      361 gccggccctg tgtcatcagg cacgcagacg aataatcatc gtcgggacat cggtataagc
      421 aaacgtgccg cgcggcagct cctcgcggac gtcagaaaac ggtaacagct tctcctgctg
      481 gcgtggtccg ttgagacaga cggcgaccag cacgccaccc ggacgcaaca gggaaaaggc
      541 ccgcaggata tgccggatat cctgcccgtg gctgaacggc ggattcatga taacccggct
      601 gtaatactgc actggctgcc attccatgaa gtcgccacac tgaaccctga caccgttaaa
      661 attttcccgc agataccgga ccagcccgct gttgatttcc accgcatcac acatggcccc
      721 cggcgcggta tcccgaatcg cccgcagtat ggccccggtg ccggcagagg gttccagaat
      781 gtgatcacgg ttgctgatct ccgccagcat caccaggcga tcacagaccg gggcaggagt
      841 cacaaaaagc tgagattcag cccggatttc agcaaccggc gcggcgttat ccagccttgc
      901 gcgcatcagc cggatacgtt ctcttaactg actgtgcata tcattatctc cgtcatcccc
      961 cggggatttt cccgggggga tataccttat ttctgtggga tttccgtgat tttcatgtcg
     1021 gtgatataca cattcaccag gcggaaatta ttgtccattg tgcggcggta ccggtatgcc
     1081 ccgtggtctt ctgcttctgc cacactgcgc acggccttac agtcccgggg cagggcggcc
     1141 cactgtgcct ttgtcatttc ccggaaacct tcccccggat agttgactac cggcggacgc
     1201 ttcgcggcct gactggcgac ggcagcctct tctgccgatg gtgctttgta gtccgtgatg
     1261 cgatcgggcg tcactttcat cgtgccgctg tacccgagaa aactgtaatt tggcgttgtg
     1321 actgaactca ccgccccgtt gcttttgttc acacggatga tggtcagcca ctcgccccgg
     1381 ctgaaaacct gtccgcccgg ctcaaaatcc tgtgtccggg taaccacgcc gccgctttcg
     1441 tccagcatgg cacgctcata gatcaggcgg ttctgatagt ggttaaccca gcgttgttga
     1501 tgctgaatct gccgttcatg acagcggatc gcaatttcgc gggcctgctc cgtggtgatg
     1561 atgtcatcat ccagggccga ccataacgat cgacttcctt catactggct tttttctgcc
     1621 gggcgcggat acgtatccag cgggaaacag gcgctgatat ggtcatgact gctgatgagt
     1681 tttgccatat tcagatccag cgattcagcc cgccacatcg tcagatattt ctgcgactgc
     1741 gcgatggtct tttcagcctt gcgcaaatca gcttcgattt ttttgatacg acgccagcga
     1801 acgtccggac gttctttata cttcgcgtga agcagtgccg accgcgcccg ctcttcccag
     1861 tattccgcac gttcaaagag catcacggct cgtttcatgc cgttttcaat acgctgcgca
     1921 tcacgacggg cgcggcgttc gctgtgatgc cccacaagaa tgggctgacc gggcgggatc
     1981 atcgcggcca gtctttccac ttcatcaagt gcctgtgcgg attcactggc ccgctttccg
     2041 ctgtatccgg taaaccgctc cgcccttgct tcctgacgtt cagcgagcgt gctgtcttca
     2101 tcctcaatct ctccggcaag tgagaggagc acgtcttccc ggcccggtgt ccaggctggc
     2161 gcgacaaaca gcgcctgttt tggtgcccag cggaaaccgg catcatgcac ttttttatac
     2221 gtctccgggt caagacgtga cacggcatac aggcgcagtt tgttgtcatc cggtgagtag
     2281 gtcgcgcggt acatctgcac agggctggcg gcttccgggg cggtcatggt gattgctgct
     2341 aatgtcataa aatgttctcc ttccgtctgt tgccgcccct cgcggggcgg ctcacctcat
     2401 cagttaaaag cgcgtttcag ttcgagtgtg cggagcacct gcccgaaggc cgtgtttttc
     2461 atggcgggga tgtgtacacc acgcccccgg tgaaaactcc gggcaatgtg tcgcccgaag
     2521 gctttcagca cggggtcaat ttccggcgcg gggatgtctt cgcgtctgaa acggggatga
     2581 cggcggggct ttacagccgc tgcggaaggt gaaacaatac ttactgtcat aatttagcct
     2641 cttatgacgt taattaatga aaccgtaaag actgttcgtg cagtctctgc ggtgtggatt
     2701 aagcaggtcc ctgacctgct tttttcatat ctgcgtctga cgttctccgg tgttttcctc
     2761 cttctgttcc tgcgttctca ttacctgcca gccggatgac tgacagaaca cataaatgcg
     2821 tattgccgga gcctgaaata aatcctgatt tttaaagaac atcacccgtt gagtttcggg
     2881 agccgcggtt ttgacgtttc gcgggaacct gctgctgtat tccctgtggt atgaacttca
     2941 cccgtttgag aaccttcgcc gcaaggggca tcactgccac ggcctgacgc acctgtcctg
     3001 ggcagaacgc aaaatttttt accgggtttt gggtaaaaaa ttttgtgggc tgttcattta
     3061 ccctggacag tcagtgcggc aggttgtggc tgtatcagcc cccggcgaca gggactcata
     3121 cgggtgtgtg gcaccggcag gcaatgagca gggacccgcc gcaacgacgg agagcgcgtg
     3181 cgcgaaacgg cgttgcccct tacatcccgg cggccttttg ccgggatgcc gggagcgcag
     3241 gccacggcgc gacaagggtg gcgcgaccgg aacggtcgct gcaagcccgg ggttttcacg
     3301 catcggaccg tagcgacagc ccgaaaccgc gccagcgggt tcggcggaga ctggcgggtg
     3361 cgccagcagc tgcctgtcgg agtcgggcct gacgaccgga tgccgccagg cagccgggag
     3421 ccgaaggtgc gcccggcaga cagaacaaca ctgcagtagt cagaatcaga gcagcagata
     3481 aaggaacaac aacatcagga acagttccat aacataagaa agaaacactc ctgagtcaga
     3541 cacatcacag acgggaaggg taacgggcga cgcgccagcg tggcccgctt cccttccggc
     3601 aggcgtcagc ctgccagggg ttttcgcgcg gactgacagg cggcctccac cgcctgcccc
     3661 tgtatcacct gtttctgcta ccctgtaaga tgtagtgacc gcccaggaaa accgcctgct
     3721 gattcaccac aaaatcgcag atttcatcaa caatctgcgc ggtggtgagc ggtttctgcc
     3781 ccatttcacg acgccgcgca ttaatcacgg ccctgtaatt acgcagaatt ccgcgggttt
     3841 ctgtgctgat attcatgaca tcgtctcccc gtccagcgcc tgtaacacaa cgtccattgc
     3901 ttcgcgttcc tccctgacga atgcgtcacc gtcgcccccg ttctcactcc cgacggcata
     3961 caggcgcttc atttcactgc ctgccagcgc catcactttt tccagcatca ccgccgggac
     4021 tgtcacctga cggctggcgc gcttttcttc catcagttca cgaacaacgc cgtgaacaac
     4081 gctgacatcc gcaccatgct caaaggcatc gtccagtcgc tgcatgacgg tttccagctc
     4141 atcgtctgac aggttccagt cctgggctat gtgacgcaca tcccctcggg tcagcaccac
     4201 ggcaaaaacg cattcactgt ccggcacgtc tgtatccagt acatcgcgga tgatttcttt
     4261 aacagtacag tacatgttct cgtctccttt acaggggcgg cattgccgcc ccgtccgtta
     4321 atcagtccag tgctgcgtaa atcgcgttgc attcagggtg aaattcgatg tgacgccaca
     4381 actgcgcatc gcgcatccgg taaagctggg tcagtcctgc atccccgctg tcgtggtaca
     4441 gccacaactg gcggttaatc acaagggagg taaggatgat gcctgcggca tcagcactca
     4501 cggtacggtc aaaccagttt gcaccgttca ccatgtggaa acggtcgcca tccggcatca
     4561 tgtaaccgcc accgtccggc aggcggatga agtgccagaa gcctccctga tactccggca
     4621 tcattttttt cgccagcgcg tacacatacg cctcggcata cagaaaatcg ttgccaaaca
     4681 ggtaaggcag aaaggagaga cggtcgcact cttcaacatt cagagtgaca ggtttcgcat
     4741 attgcatgat gttttcctcc gttattcaca tattccgcga caccgggcgg tgccgtgcct
     4801 ccataaaagc cgggcgataa ggcggggatg cacagggcgc aagggaagcc gcagcctgac
     4861 gcacacggct ggcgggagtt ggagggtgag accgcgaaat ctgagcgggt gaacgaccgg
     4921 aaaatcacgg cagacgggaa ggagggaccg ggtgaccgca gcggcgaagc gtacccttgc
     4981 atccctgcca gcccgactaa gatggaggca cggcacagcc cgcacacgac acagcggcca
     5041 cataactgaa aacccacaag gccatgcccc gcatggccgg gtaaagcgga gccggcggcg
     5101 acttccttag tccctgattt ttgccagggc atccgccatg ctgcggattt ctacccgggc
     5161 ttcccggacc acctcccgca gaacaccacg gtcaaaccag cggcggggcg gttcatccgg
     5221 tatacgcagc acatcgcagg ccagcccact ggacaggata tgctcaatac aatcgctgac
     5281 caggtacatt tccaccgacg aaaaacgtgc tccccggttc acgatggcat cgagcaccac
     5341 gggcaggttt tcgtcatctt catcgagcca gcaggggtga tcgctttcct tcacaaccag
     5401 acgaaagcgg cgcgtctggc gggtcagcaa cgtctgaccg gtggtggtca gcgccgcccg
     5461 gatacgccgc gcatcgtctg ccacctggta acagatgctt cgtattgcat cgcgcaccgc
     5521 cgcaagtaca gccctgattt cgtcttctgc cagccactca ccggcatacg ggaaagacac
     5581 ctgcgttccg tctgccggaa gtaccggcag cgactgccac tgactgccgg acagaacaag
     5641 ttccgccccg gttccggtca gtttctgaat ggcagaggcc aggtgcggcg gcagtacgcc
     5701 gccgtgacgg ttgataagta acgtcgccag ccggggcgca tccagccgca cgtaaatgct
     5761 gatccagccc ggatggttcg gatttcgctg gtcaacatcc gtctcttccg gccagccctg
     5821 cgccagcagc aggttacggg cgtggcgaca aatcagtgca ttcagcgttt cgttcatgat
     5881 tcatggtctc cctgaatcgc cgcccgcagg cggcgataac atcagtcgtt ttcgcgtgtg
     5941 ttgtttttca gcagggcgcg ggcctgatta accaccgcct gtcggcgggt gacgcattcc
     6001 gggacgacga ggccgttatc acgggcggcc cactcacgtt tccacagtgc ctgttcagcg
     6061 gtctgaatca gggacgccag cagttcagca gggacggtca cctggcggtt ttcgctcacg
     6121 ttgctgataa tctccatcac cgcggcggaa gaaataccgg attcagtcct ctggtcttcc
     6181 gggatgtcct ccatgcgcgc caggacggcc ctgatttcat gatcggtcag agaaatttcc
     6241 ataccgtcag ccagtgcctg aatctcttcc ggagaccaga taaccagcat cagcggcgtg
     6301 tctcccggat actttgcttc cagctcccgg cagagcgttt cgcatgttcc gtacatttcg
     6361 tctccccctt aatctttgaa gcacgcatcc agttcaagaa tttcggtata tgcctccatg
     6421 aacaggcgat tggcgcgata cagcgtctgg cgtgccaggc gttcgacgag cacatcggaa
     6481 tcaaagcgac agcgatgctc catatcgttc agcctggccc gaaggtcaca catggcattg
     6541 gccacctccg ccacttcagc acgaaggttt tcgtgtccgg catagttcat ttacgcagcc
     6601 tccggttcaa accagttatc attcgcggcc ccctgctgca tggcccgttg cacggtggcc
     6661 agtcgttgct gtccggcacg gtgatactgt tcaagcagct cgataccgat ataccggcgt
     6721 ccggactgaa gggcagccac acaggttgag ccgctgccag caaacgggtc tagcacaatt
     6781 gcgttcgggt gtgtgaagct ctcaatcagc ggttgcaggc tggtaacagg cttttccgtc
     6841 gggtgatggc ggttgcccga atatttccag cccagcacgt cgggtaatgg cttcggaggc
     6901 agcgccggac ggccttttgc caggatatag gcgcattcgt gacggtacgc gacatacgca
     6961 gatttggatg tgtagctttt ggtgaacaca aggtgcccta caacgctgaa tccggcgcgt
     7021 ttccaggctg ccataaagcg gtcaatacgg ttccagccat agaaactcac catcagcgca
     7081 tcttttttta gtacgcggta catctcatta ctggcaggtt gcagccagtc atcgttaacg
     7141 tcaccggcaa tggtgcgccc ggaacggtca cgaaaaccaa cgagatatgg tgggtcggtt
     7201 aagatgaaat caatggcgtt atcaggaatc gtggccataa cacgcacgca gtcacccagg
     7261 ataaaacggg acataacagc actcctcctc ttacagaggg caccgggagg gcgcccctgt
     7321 ggcgcccgtt ctccgctgcc tgtgggctgt tcaccgcgtc cggtaacggg tcaggccgac
     7381 cgtggaatac cgcagccgtc gtctttctga cggcgaggat atgccgcgaa agcggcttga
     7441 agggcattca tcagaaaagg gcgacaggct ggcgtacact gtcaccgacg ccagccagga
     7501 gcggtaaaag cgcccgtccc gtgcccggcc ggacgcggga cactgaccac aggcagcgtt
     7561 cctgccgcgc cgcgtcagcc ggcgcagtaa aaagagaagc acagcgacat cttttgttac
     7621 ccgccaaata aaacccaaaa acaacccaaa acacaccata ataaacccaa aaacaataca
     7681 aacaatcatt gactgatagt caaaatgggg taaacttgac aaacgacccc atttaaaacc
     7741 caaaacaacc ccaaaagcac accacaaaaa caccataagg agttttataa atgttggtat
     7801 tcattgatga cggttcaaca aacatcaaac tacagtggca ggaaaacgac ggaacaatta
     7861 aacagcacat tagcccgaac agcttcaaac gcgagtgggc agtctctttt ggtgataaaa
     7921 aggtctttaa ctacacactg aacggcgaac agtattcatt tgatccaatc agcccggatg
     7981 ctgtagtcac aaccaatatc gcatggcaat acagcgacgt taatgtcgtt gcagtgcatc
     8041 acgccttact gaccagtggt ctgccggtaa gcgaagtgga tattgtttgc acacttcctc
     8101 tgacagagta ttacgacaga aataaccaac ccaatacgga aaatattgag cgtaagaaag
     8161 caaacttccg gaaaaaaatt acattaaatg gcggggatac attcacaata aaagatgtaa
     8221 aagtcatgcc tgaatctata ccggcaggtt atgaagttct acaagaactg gatgagttag
     8281 attctttatt aattatagat ctcgggggca ccacattaga tatttctcag gtaatgggga
     8341 aattatcggg gatcagtaaa atatacggag actcatctct tggtgtctct ctggttacat
     8401 ctgcagtaaa agatgccctt tctcttgcga gaacaaaagg aagtagctat cttgctgacg
     8461 atataatcat tcacagaaaa gataataact atctgaagca acgaattaat gatgagaaca
     8521 aaatatcaat agtcaccgaa gcaatgaatg aagcacttcg taaacttgag caacgtgtat
     8581 taaatacgct caatgaattt tctggttata ctcatgttat ggttataggc ggtggcgcag
     8641 aattaatatg cgatgcagta aaaaaacaca cacagattcg tgatgaacgt ttttttaaaa
     8701 ccaataactc tcaatatgat ttagttaacg gtatgtatct cataggtaat taatgatgga
     8761 caagcgcaga accattgcct ttaagctaaa tccagatgta aatcaaacag ataaaattgt
     8821 ttgtgagaca ctggacagta tcccgcaagg ggaacgcagc cgccttaacc gggctgcact
     8881 gacggcaggt ctggccttat acagacaaga tcccagggcc cctttccttt tatgtgaact
     8941 gctgacgaaa gaaaccacat tttcagatat cgtaaatata ttgagatcgc tatttccaaa
     9001 agagatggcc gattttaatt cttcaatagt cactcaatcc tcttcacaac aagagcaaaa
     9061 aagtgatgaa gagaccaaaa aaaatgcgat gaagctaata aattaattca attattattg
     9121 agttcccttt atccactatc aggctggata aagggaactt aatcaagtta ttttattggc
     9181 aattattaca taaaataaat acagcgtcgt tattatgaaa ttatttcacg gcacttatac
     9241 caataatcaa acttggtgag ttcgcaatgt ctggcaataa cgtttttgat gggttgtttg
     9301 ccagcccaga gcgcgatatt gccgccagtc acggaaacac cgtattcata taccatgttg
     9361 atgatgataa aatagctaaa tccagcgatc ttgatgctcg ttttcaagag gtttatgcct
     9421 tcttacataa cgaactggat actgctgacg tagaggaaat agctgatcgc gtcatgtggg
     9481 ataacaattc agatatcgaa gatttcgcag atattctctc gccacgcctc ggcagcgata
     9541 ttaatggagc atactcatgg gaattgcaac gcctgcgtgg tcgtgtagcc gcttatcttg
     9601 gatttgatgc catagaaatg aacgatgaat acggcacaag ttacctcatt gtgaatccac
     9661 aaattaaaga tgaataaaat gaattcccgc attagaggga ccagatattc tccagtacct
     9721 cctgtcctgc aaggctatac ggtctgtcag caatgaaaat tgctgtacgc ggttgaagaa
     9781 tttacccgca gccgggccgt gagggccgct tacgaacgtg tatgacagag ttacatttat
     9841 acaatgtcct gcagggaacg gacatcggca ttttgcggca ctgcgctgac cggaagttgc
     9901 cctaaaatgt aaaccgcggc ccggtccggt aacggtcatc tgcagccagc attctttcag
     9961 ccagagaaaa atcgttttca ccgcagaaca cgtactgttc agcatactcc acctgcagcg
    10021 cgttataatg catcacattt tctgcagaaa gtggcataag aagatagtcc gtatgcctgt
    10081 tgacatgctc gagcatttca aacgggcgca tgtgacgcgg gattagatcc ggcgctctgg
    10141 caataagatg aaggagatgc tgcttttcac gaactttctg ttcacggatg gaagggcaat
    10201 acatggccag catcagcgtc gatgacaggg gcagatagat ttgtatgccg ggcacggcta
    10261 ggccgatatt accgtacaca ccgaagtcat tgcggttctc cagtaccacc ggattgtctg
    10321 acacatagaa cggatgttct ggcctggttt ccaggaggta ccagtcctta cttagcaggt
    10381 gtttaatatg cgacacctgc tggacaagct gccgcagaaa aatgtctctg gtatcgcccc
    10441 cgggcgataa acccagagtt tctcctgcct gctcctcagt cacgcccatt gccgccagct
    10501 tatcagtcag caccgaaatc atgcctgaga tacgttccag ctcaccgaag gtacgtgcgc
    10561 gctgcacggc aacaaacacg gccagcttgt agcggtcatc ttcagtcagc cggcgaatgt
    10621 ccttatgctc cagtaggccg gcaatgaccg gtgccgcgtc atgttcgtaa atccccaaca
    10681 acggttcaag actgaatcgt tgagggtgat tatcgaagtt gtagaacgta ttccggacgg
    10741 tggcgtcatc cggcgtggtg gcataagcac gcccggcagc cttatcaaag gcgtgtaaac
    10801 gctggcgttt tccctttacc ggcttgctga agtgcttcag taaaaaacgg gggactgtgt
    10861 gctgcctgac aacgtcggtc cgcaggttta atggttcggt catggaaatt atcatccttg
    10921 atggactggt actgtagatt caccgtggac cagcgaatct attatgtggt gagcagaaca
    10981 ttaacacatc aatgtaacgc cgtaatcatt gagtctttgc cggggacgct tgacatctcc
    11041 gaaagaatta tatcgtgagt cttaagggga atctcttgct tccggttata catttaaccg
    11101 gatctagcta taagactgtt acatctattg ggattaggtc aggacagata gcctgaaagc
    11161 ttttatagtg agggacttca gaaataccct agaaaaggaa ctgttatggt aggttcgcgc
    11221 tggtataaat ttgattttca taaccatact ccggcttcgc atgattacaa aattcctgac
    11281 atcagcccca gagagtggct tctggcttat atgaaacagc atgtcgattg tgttgtaatc
    11341 agcgatcata acagcggagc ctgggtcgac gtgttgaagg gtgagctgga gaatatgtcc
    11401 cgggacgcca gcaccggcga cctgccggaa tttcggccac tgacactctt tccgggggtt
    11461 gaactgacag cgaccggtaa cgtacatatt ctggctgtgc tgcacacgca cagtacaagt
    11521 gccgatgtgg aaaggcttct ggcccagtgc aataataata gccccattcc gagtgaagtc
    11581 cctaaccatc agctcgttct tcaactgggc cccgccggca tcatcagtaa tatccgccgt
    11641 aatccgaagg ctgtttgtat tcttgcgcac attgatgcag ccaaaggtgt cttaagtctg
    11701 actaatcagg cagagctcac cgcagccttt caggaaagtc cccatgccgt tgagattcga
    11761 caccgggtgg aggatatcac cgacggaacc cgccggcggc tgattgataa tttaccgtgg
    11821 ctacggggct ctgatgcgca ccatcctgaa caagccggca tgcgaacctg ctggctgaaa
    11881 atgtcatccc ctgattttga cggactcagg catgcactgc tcgatccgga aaactgtgtg
    11941 ctgtttgatc agctccctcc ggaggaacct gcgtcatatt tgcgcagcct gaaattcaga
    12001 acccgccact gccatcctgt gggtcaggat tcggcctcgg tggaattcag cccgttctat
    12061 aacgctgtaa tcggctcaag aggcagcggg aagtccacgc tcattgaaag cattcgtctt
    12121 gcaatgcgca aaacagaagg tctcactgcg acccagggga gtaagctgga ccagttcatt
    12181 cggacgggga tggaagcgga ttccttcatc gaatgtattt tccacaaaga aggcacagat
    12241 ttccggctca gttggcgacc agacagtaag catgaattac atatcttcag tgacggagaa
    12301 tggatgcctg acagtcactg gtcggctgac cgttttccac tctcgattta cagccagaaa
    12361 atgctctatg agctggcttc ggatactggt gcattcctgc gcgtctgtga tgagagcccg
    12421 gtggttaaca aacgggcctg gaaagagcgc tgggatcagc tggaaaggga atatctgaat
    12481 gaacaaatca cgttgcgggg cctgcgtgcc agacagggaa gtgcggattc gctgcggggg
    12541 gaattatcgg atgctgaacg tgccgtcagt cagctgcagt caagcgccta ttatccggtt
    12601 tgcagacagc tggccctcgc cagaaacgag ctgtccgcag caaccttacc cctggagcac
    12661 tttgagcggc gtattgcagc cattcaggct ctggcagaag aaccgctgca gagatccgat
    12721 atcccgccgg aaccttccgg tctgctgatg gcatttatgg cgcgcctgtc atctgtgcaa
    12781 cagcagtatg accagcggct caatactctc ctggcagaat atgctgcaga gctcgcgggt
    12841 atcaggagag agcaatcttt tattgccctc cgaacagcag tgagtgacca ggaaacaaat
    12901 gtagaaagtg aagctgtttc cctgcgggcc agagggctta atcccgatgt tctcaacgaa
    12961 ctgatggcac gctgtgagtc actgaaaaat gagctgagaa attacgacgg tcttgatggg
    13021 gcgatctctg cctctgttgc acggtctgag cagttgctgg ctgaaatgcg tgcccacaga
    13081 atggcattga cagataaccg gaaggcgttt ctctcctccc tgtcgctcag cgctctggaa
    13141 atcaaaattc ttcccctctg cgccccttat gaagatgtta tatctggtta ccagacggtt
    13201 accggcatca gtaattttgc cgaacgtatc tacgataaca gtgacgggag cggattactg
    13261 agcgacttta tcagtgaacg tccgttcagc ccgttgcctg ccgcaacaga gaaaaaatac
    13321 agggcgctgg acgagctgaa agcgctgcat cacagcatcc ggctggataa ttcagaggct
    13381 ggggcggggc ttcatggttc tttccggaat cgtctcagga gtctgaatga ccagcagctg
    13441 gatgccctgc aatgctggta tcctgatgac ggcatccaca tacgttacca gacccccggg
    13501 gggcagatgg aagacattgc ctttgcttct ccggggcaaa agggagcgag tatgctgcag
    13561 ttcctcttat cctatggcac cgatcctcta ctactggatc aaccggagga tgacctggac
    13621 tgcctgatgc tgagcatgag cgtgatccct gccatcatgt cgaacaagaa acgccggcag
    13681 ctgattatcg tgtcgcactc tgcccctata gtggttaacg gcgatgcaga atatgttatc
    13741 agtatgcagc acgatcgcac aggcctgtat ccaggactct gcggtgcact gcaggaagct
    13801 ccgatgaagg cactgatatg ccgtcaaatg gaggggggag aaaaagcgtt tcgttcgcgc
    13861 tatgagcgta ttcttagctg aagaacggaa ccgtccttaa ggcggccacg accggagagt
    13921 gggcctggcg gctgaatgcc tggataaaag acgcaaatgt cagactgatg gcctctgcgt
    13981 ctttgtcagc gccagtgata taagacggta atggggatgc ggacgatttc ctggactctc
    14041 aggagatgaa acatgtttac tgaaaaacaa aaaatccgcg ccattgagct tttttgtcgg
    14101 gataaaacgg aaagccgctg gccagccagc tgttgtttac tcgtcacgcg acaggcaagc
    14161 ctgttcaaaa aacaggcttg ccgtacaata attctctata tccaaactga ccccttatag
    14221 agagcctgac agaggctgcc atgatatggg cacatcaaga atgacaatcc acactcgtgt
    14281 tgttctatct gctcaaccag caagatatca ttgcgatgac tgtccatcag taagcgtccc
    14341 ggttccgggc ggtctagttt tgtgccactg atgttctccc cgcagtggtt tctaaccata
    14401 gtatctgtgg taactttcac gtgctttttc aaaaagttgg catagtactt tactgttttt
    14461 ttgagtccaa ctttttaata catccagatg aatatagaaa gatgtctcat cacctgttgc
    14521 tatcagtact gaaaaatttc tcaaggagat tgaacatgtg aataatacat gtctgaataa
    14581 aatattttct tctgaatttg ctttatgaca aaacgctatg acagaacctg gtaaaaatgt
    14641 aatatttcgc attgtatgat tcagatcaga taataataaa ctcgaaatcg gataaatgcc
    14701 ggagttttct atatatcccc ttccatgcaa ttcaaaatca tatggtatat atttatcttt
    14761 agtt
//
LOCUS       DAFNBJ010000119        14756 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000119 DAFNBJ010000000
VERSION     DAFNBJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.119"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            692..1105
                     /locus_tag="L2X49_003480"
     CDS             692..1105
                     /locus_tag="L2X49_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798508.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLCWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRCGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            1107..1886
                     /locus_tag="L2X49_003481"
     CDS             1107..1886
                     /locus_tag="L2X49_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBN4798509.1"
                     /translation="MQYLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPVGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVTAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            2066..2710
                     /locus_tag="L2X49_003482"
     CDS             2066..2710
                     /locus_tag="L2X49_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBN4798510.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            2797..3105
                     /locus_tag="L2X49_003483"
     CDS             2797..3105
                     /locus_tag="L2X49_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HBN4798511.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            3519..4499
                     /locus_tag="L2X49_003484"
     CDS             3519..4499
                     /locus_tag="L2X49_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBN4798512.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            4492..4908
                     /locus_tag="L2X49_003485"
     CDS             4492..4908
                     /locus_tag="L2X49_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBN4798513.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(4910..6184)
                     /gene="umuC"
                     /locus_tag="L2X49_003486"
     CDS             complement(4910..6184)
                     /gene="umuC"
                     /locus_tag="L2X49_003486"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HBN4798514.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGVNRCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGELQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(6184..6621)
                     /gene="umuD"
                     /locus_tag="L2X49_003487"
     CDS             complement(6184..6621)
                     /gene="umuD"
                     /locus_tag="L2X49_003487"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBN4798515.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(6618..6866)
                     /locus_tag="L2X49_003488"
     CDS             complement(6618..6866)
                     /locus_tag="L2X49_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBN4798516.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            7284..8186
                     /locus_tag="L2X49_003489"
     CDS             7284..8186
                     /locus_tag="L2X49_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBN4798517.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            8190..8495
                     /locus_tag="L2X49_003490"
     CDS             8190..8495
                     /locus_tag="L2X49_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798518.1"
                     /translation="MTETGGKPPVSFPTKDVAVLLFLLRRLTRRGINAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            8571..9254
                     /locus_tag="L2X49_003491"
     CDS             8571..9254
                     /locus_tag="L2X49_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBN4798519.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFVAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            9255..9476
                     /locus_tag="L2X49_003492"
     CDS             9255..9476
                     /locus_tag="L2X49_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798520.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            9489..9923
                     /locus_tag="L2X49_003493"
     CDS             9489..9923
                     /locus_tag="L2X49_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBN4798521.1"
                     /translation="MYGTCETLCRELAAKYPGNTPLMLLIWSPEEIQALADGMEISLT
                     DHEIRTVLAHLEDIPEDQRIESGISSAAAMEIISNVSENRLVTVSAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPESVTRRQAVVNQARSLLKNNTHEND"
     gene            9969..10745
                     /locus_tag="L2X49_003494"
     CDS             9969..10745
                     /locus_tag="L2X49_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798522.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIY
                     VRLDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLDAVHDAVQSICYQVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLIVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSECVEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRG"
     gene            11279..11581
                     /locus_tag="L2X49_003495"
     CDS             11279..11581
                     /locus_tag="L2X49_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBN4798523.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DACGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            11628..12050
                     /locus_tag="L2X49_003496"
     CDS             11628..12050
                     /locus_tag="L2X49_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBN4798524.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMVEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            12047..12238
                     /locus_tag="L2X49_003497"
     CDS             12047..12238
                     /locus_tag="L2X49_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798525.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQDSRNR"
     gene            complement(12516..14183)
                     /gene="ltrA"
                     /locus_tag="L2X49_003498"
     CDS             complement(12516..14183)
                     /gene="ltrA"
                     /locus_tag="L2X49_003498"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBN4798526.1"
                     /translation="MNTHISVSTIPHPTGWHTINWKACHARVRKLQLRIAKATRQQQW
                     RQVRELQRILTRSFSGKAVAVRRVTENTGKRTPGIDGKIWHTPKEKWEGICSLNLCGY
                     RPQPLRRIHIPKSNGKTRPLGIPTMRDRAMQALWLLALEPVSETTADHNSYGFRPMRS
                     THDAIESIFLRMSQKVSPKWILEGDIKGCFDNISHDWLLSHIPMDRRLLKKWLKAGYM
                     ERGVFNHTNSGTPQGGIISPVLANMALDGLEKELMQTFRKSGYHSAKHQVNYVRYADD
                     FICSGSSRELLENEVRPLIAAFMRERGLELSEEKTAITHIDKGFDFLGQNVRKYNGKM
                     LIKPSKKNLKNFLCKVREIIKRNPTLPAWKLIGQLNPVIRGWATYHRHVVAKETFNYV
                     DTQIWRAIWRWCVRRHPRKGLRWIAGRYFSFEGRRWIFKAITPEGKILTLFRAMETPI
                     KRHIKIKGEATPYTPGMEIYFERRLDLIWKGKSKKMKTVVQLWKRQGKHCPQCGQLIT
                     NQTGWNIHHRIRKVMGGSDELTNLELLHPNCHRQLHSREAGAHRKHL"
BASE COUNT         3258 a         3753 c         3974 g         3771 t
ORIGIN      
        1 ggctttggct gactcgcaga gataaatcgc gccctggctg tccagattgc attcccggat
       61 gcgtttttcc gcaaaccggg tcagttgttc atagctgagt gaaaacaccc tttcttcatc
      121 catcctgcac actccatctg ataatctcct gttaagaatt aaatcatata tgaactgatt
      181 tatttctgtg tatgtaaata tcatatcagg tatgattgta tgattaatga taaatatcat
      241 atctactatg attttatatt ttttatctta taatcatatt aattgcccgt ttcccgccgc
      301 gcagatccgg tttccggcgg agacggatct tcctctcccc ccacacaacg ccacctcctg
      361 tcagcacaac atgtagtgct gaattcagct gctaatgaca ctatatgttg tgtcatctcc
      421 ctggcctgtg atgcgccgcg caggggcgga aaacagcgat atgattattt tctcagcgtg
      481 gtacacttcc ggaaagttgt gatattccgg aaagtcggat ctgacggaaa cggctctccg
      541 gtaatttaac ggcgtggtta tatggatgct tgttatcatg gtgatgatga taacggcatg
      601 atgttatcag acggcgtgac ggtaagggca gtgatgatgg atgacgttat cgcatgaccg
      661 tccctgcccg gaaaagaaaa aggagtcacc catgtttttt attgagaatg aaggtcaggc
      721 tgtcgccgga acggattact ggcagtctgt acaggcgcag gccggatatg tctacctctg
      781 ctggaatgcc ggcgcagcca ggctgcttgt cccggatgcg gcaaaacatt tactcaggga
      841 gatgcggggg gctgagtacg tcatcatcag taagggaacg ctgcatggcc gcgatgcgct
      901 ggaactggta tttgaagacg gcagcgatgc gccgtttgtg atccacatgc tgagtgagca
      961 gtgcgatcgc ctgctccccg aaaacaacca gggcgggggt tttgttgtca ccgtctggac
     1021 gcgttgcggt aaccagctcc gttatccggg aaagtaccgt gttgtggaaa acctgcccga
     1081 cgtttccccg tggagtgaac actgatatgc agtacctgcc ggcaccgatc caccatgccc
     1141 gggatgctgc tcagcttcct gttgccatcg attatccggc agcgctggca ctccgccaga
     1201 tgtcgatggt tcatgatgaa ctgccaaaat acctgctggc ccctgaagtg agcgccctgc
     1261 tccattacgt cccggatctg cgccgcaaga tgctgctggc cacactgtgg aacaccggtg
     1321 cgcgcattaa tgaagcactg gcgctgacgc ggggggattt ttcgctcacg cctccgtatc
     1381 cgtttgtgca gctggccact ctgaagcagc ggacagaaaa agccgccagg acggcaggaa
     1441 ggatgcccgt cggtcagcag actcaccgtc tggttccgct ctccgacgcc tggtacgtca
     1501 gccagctgca gacgatggtg gccacactga aaatccccat ggaacggcgt aataaacgaa
     1561 caggcaggac agagaaagcg cggatctggg aagtgacgga cagaacggtc aggacctgga
     1621 ttggggaggc ggttaccgcc gctgccgctg atggtgtgac gttctctgtc ccggtcacgc
     1681 cacatacgtt ccgccattcc tatgcgatgc acatgctgta tgccggtata ccgcttaagg
     1741 ttctgcagag tctgatgggg cataagtcca tcagctcaac agaggtctac acgaaggtgt
     1801 ttgcactgga tgtggctgca cggcaccggg tgcagttttc gatgcctgag tccgatgcgg
     1861 tgtctatgct caaaagaatt ccataagctc tgactttttt aaatcccttt cggatatctc
     1921 atggatatat tatcggtatt tcatattctg ttgacatgca ggaccggtat agagtaaaaa
     1981 aacatctatt gagtacctct tggatatccg aaaggtgtct tatgggggct ttatgggtat
     2041 tacatgtgga ttctggagat acactatgcc tgttattgct aatacgcatc ccaaaggtgg
     2101 tgtgggtaag acaacttcat ccgtaaacat tgttggtgaa atgaagtctg atacagttga
     2161 tctggatact cataccgggc tttctatcat tctggggctg agacctgaag gaaaagaaat
     2221 ttccgtgaaa gtacccaaaa cagtggatga gttaatcgaa attatgactc cctacaagaa
     2281 cagcgataag acgttactta ttgactgtgg gggatttgac tcagatctta cccgtactgc
     2341 gattgccttt gcagactgcg ttattgtccc ttcaaaagac tccctgactg aacgtattgg
     2401 tttgatgcat tttgatggag tactggacga aatcagctct atcatgggga ccgatataac
     2461 tgctcacctg tatctctgta aagttaatcc taacaagaag aaattcccta agcttgatgc
     2521 aatcctgcca tcgttcaaac atctgaagct gatgaagagc cgcatttctg ctcgtgcaga
     2581 atttgaggat gtcattgaga ctgggatggg aatcactgag tctgttcatg ggcgctattc
     2641 ggcaggaggt aaggaagtta ttgccctgat cgaagaaatc aatcatctga ttgaaaataa
     2701 caaacagtaa ggtgtgtcta ttgggtatcc atatagtatc ctttgggctt ttaatggata
     2761 tcttttaagt atctaacaaa catcctgggt tatctcatga gcaaagcgaa atttagtgca
     2821 caggctgaaa aactgaaaaa cctggtggat tctgccagat tacaaacttc ttcttctgcc
     2881 gtaacagcaa cagccccgtc tcagccaaat gaaggtgcag aggaagtttc aggtcgtccc
     2941 gtggctgcca ggacgaaggc taagaatctt aaggccatac cgctgtcgta ttttgaagct
     3001 catgcggaac ttaaagcgac cagtaaaaca tctctggatt ttagctcata tattattgag
     3061 gctataagag agaagcttga acgtgatggt gcaataggtc aataaactat cggatacctt
     3121 aagggtatct gatgtgtgtc ttttaaatgc gttatgggga ttcttcacgt acctaaagag
     3181 tccccattag acatctttct gtttatggtg acatgattcg tcaccgcttt cctttcctct
     3241 cagtttcacc atttcattcc ttttccccta acctgaacca tgatgtccac gatgttttta
     3301 tattacattt atgtatcaca tataaccttc ataatgtttt tgtatctatg atttcctctg
     3361 gtcttaaagt tgtgtgtaca atgtacatgc aatttatcgt gtttttatgt tgcatttgtc
     3421 ctttcagaaa gaccgttttt tcattataat tgaatataca ttgcatgtat gaagcatgtt
     3481 tgttttttct atttgtttat gagcaacgga gactggttat gaaaatcttt attgatgatg
     3541 gttctaccaa cattaaactt gcctggttgg aggatggtga cgtgaaaacg ttaatcagcc
     3601 ctaacagctt taaaccggaa tggtctttta gtttactgga tgatgctgcc ccggctaatt
     3661 acgagatcga cggcgagaag ttttcatttg atccattaag tgctgatgct gtcgtaacga
     3721 ctgaaacacg ctatcagtac agtgatgtga atgttgtggc cattcagcat gcgctacagc
     3781 agaccggcct gaaagcgcag cctgttgatg tcatcgtcac attgccgatc tctgaatacc
     3841 ttgacgccaa caatcagaaa aataagcaaa acattgaaag aaagaaaaaa aatgtgatgc
     3901 gtgaagtacg ggtgcagggg agtgatgcat ttgttattcg cagtgtttcc gttcttcctg
     3961 aaagtattcc cgctggtttc agtgttcttg cggggcttga ggatgatgaa tcccttttga
     4021 tcgtagatct cggtggtaca actcttgatg tgtcgcatgt tcgtagcaaa atgacaggga
     4081 ttacaaaaac ctggtgtgat ccaaatatcg gcgtgtccct gattacatcc ggggttaagg
     4141 aacagatggc tgtacatgct aatacaagag tgagctcatt ccaggctgac aatatcattg
     4201 tgcaccgtaa tgaacctgac tacctttccc gtcgcattta taatgctgaa cagcgtgagt
     4261 ctatcattaa tgttatcaat gaacggcaga agttgcttat taaacgagtt aatgatgtca
     4321 tcagccgttt caccgactat acacatgtaa tgtgtgttgg tggtggggct gaaattgtgg
     4381 ccgaggctgt gaagaacctg actaaggttc ctgatgagcg cttttatctg agctcatccc
     4441 cgcagtttga tttagtcatg ggaatgataa aaatgaaagg tggtgtgact aatgagtgat
     4501 gaaaataaat ccagacgctg ctcatttgaa cttttcccgg atgaacggac aggagataag
     4561 atagcagatg agctcattgc aaatgaaaaa ctgaaggagc ggggacgttt catgcgcgcg
     4621 atgttagtta ctggtgcggc gtttgcagct atagataaac gtcttccttt gctgatatcg
     4681 gagttactga cagaaaatac gactctggat gatattaata aggtgatttc cagcgtgata
     4741 ccaggtgctt tttcggttga aaagaaattg cttgaattac tggaaaaaca atctggtctc
     4801 catacgtctg tggattgcag tactcctctt acagagcaga gtctgtcgag aaatgacggt
     4861 gaagaccaga ccagaagaaa cgctgaaaat atgttcggag atgactgaac taacccagcc
     4921 tggccagggg aatatctcgc catttagttg tataacactg actcaacatt tcccgtttca
     4981 tttgccattc aggggcggtt cctcgccccg caaaccacac tttccctttt cccgactggt
     5041 tcagttcatc gagtgttttc attaactttt cactgttttt acggggctgg atttcatcaa
     5101 ataatcccgg ctgcgctata cccgatggtg tgaaatcagc cagcatgact cctgccttca
     5161 tatagcggta cccttcacgc cagacatggt ttaaggctct gcatgcggcg gcaataatgt
     5221 cccggctgtc ctgtgtgggc aatggaagct tttccacagc ggcattgctg taacagggtt
     5281 cttttactgc aaagggggat gtccgtacaa atgtcgtcac ctgccggcaa tactgaagct
     5341 ccccacgtag tttctctgcg gcccgctctg catactgaac aacagcctgg tgcatggcat
     5401 ctttgtctgt gattcgttca ccaaaactgc gactacagac aatctgctgt tttgccggtg
     5461 gtgcttcttc cagggatatg caggactcgc cgttgagttc gcgtaccgta cgctcaagaa
     5521 tgacgctgaa gtttttccgg atgaatgccg tgttagcctg cgccagctgc agtgctgtgt
     5581 taatacccag tgcattcagc ttttccgtta gtctgcgtcc tactccccag acctcaccaa
     5641 ctggctgcag ccccagtagc ttcaagatcc gattacggtt ttctgccgtc agcgcgacca
     5701 caccggaaaa ctgtggccat tgctttgttg cccactgtgc acttttagcc agcgtttttg
     5761 taggcgcaat gcccaccccc atggtgagtc ctgtccagct ctttacctgt tccctgagct
     5821 gatgaccaaa aacctccgga gagatgcaac ggttcacccc ccgcaaatca atgaacattt
     5881 catcaattga gtagggctca actgcgggag aaagcgactc cagaacagcc ataacccgtt
     5941 ggctcatgct gtggtacagc gcataattgc tggaaaatac atgtattttc ttctccaggc
     6001 gcatttgtct cacctgaaac cagggctgcc ccattctgat gccaagggct tttgcctccg
     6061 ggctgcgcgc gatcacacag ccatcgttat tgctgagtac gatgaccggt tcgttgcgaa
     6121 ggtccgggcg gaaaactttt tcacatgagg cgtagaaact gttgatatca gccagtgcaa
     6181 acatcagcgt aactcccgcg tcctgtgtat cacgtgagtg acaacaccaa aaatacagat
     6241 gttttccgga tacagtgtgc ggaactccgg gctgtctgaa accggctcca gtgccgggcg
     6301 tgggcgcaac agcagtcgtt tgacggtgaa ctcaccgtcg atctcagcga taacgatgtc
     6361 cccgtgttgt ggtttttcgg ccctgtccac taccagcaga tcaccattct gcacgccagc
     6421 ctggttcatc gattcaccgc tggcgcgcag aaagaaggtg gctgcaggtc tgctgatgca
     6481 atagctgttc agatccagtt cctgctcagc ataatcagtg gcaggcgaag gaaaaccggc
     6541 ctggcaacga tcggcaaaca acgggcgcac gtaactgtca tcgcctgaag ggtcagccgg
     6601 acggtgataa acggtactca tgctgcaggc agccagctgt cgtcctgcca gatttcttcc
     6661 agcagagaca ggatacgttc tttatccttg tcgttgcggg ctcctgtgac agatacagac
     6721 ggggcgctgc cctggtctat acgcagccag atttccgggt agtgtggttt cagacgttgt
     6781 tcgatttcat tctgtagcgc ctgaagtgtg cctgatttga ggttttttgt gctctggcgg
     6841 tcgaaaagaa tttcaatgcg gatcatgttc tcatctccct tttctattgc tgtatgcata
     6901 tacagtattc taataactga atccacacac agtcaaatgt cattattgtg actaaaaaac
     6961 gctcaattct ctgagggtgt ggcggtatgc ctgctgtgga ctgctgtgtc cagcttatcc
     7021 acagcatttt gcgcacggtt ctgtgaataa aatacctggt tacccaggcc gtgccggcac
     7081 gttaaccggg ctgcatccga tgcaagtgtg tcgctgtcgc cggcctcctc acccggtcac
     7141 gtttcgtcgt tcctcctcca cgcgctgcgg cttcggggcc gcacctgcat tcgtatgcgg
     7201 tcgcccggtt acaggtgcgg cacggcctga tggaggccgc atgtgagagg agaattccca
     7261 tgccaaactg gtgctcgaat cgtatgtatt tttctggtga accggcacag atcgctgaga
     7321 ttaaacgact ggccagcggc gcagtcacac cgttttatcg ccgcgccaca aatgaaggta
     7381 ttcagctgtt tctggccgga agtgccggac ttctgcagac cactgaagat gtgcagtttg
     7441 aaccgtgccc cggactgacg gctgccggac gtggtgttgt atcgccggag aatatcgcgt
     7501 tcacacgctg gctgacacac ctgcagaacg gtgtgctact ggatgaacaa aactgcctga
     7561 tgctgcatga actctggctg cagagtggta ctggccggcg tcgctgggaa gaattaccgg
     7621 atgatgccag ggaaagcatc accgcacttt tcaccccaaa aagaggtgac tggtgcgaca
     7681 tctggagtaa cgaggatgta tcggtgtggt ggaaccgtct gtgtgacaac gtactgccgg
     7741 aaaaaaccat gccgtttgac ctgctgacgg ttctgccgac tcgcctggat gttgaagtga
     7801 atggctttaa cggtggtgtt ctgaacggtg ttccttctgc atatcactgg tatacggaac
     7861 ggtatggcgt gaagtggcct gtggggtatg aggtgaatat cagtagtcag ggagacaact
     7921 tcattcaggt tgatttcgac acgccgtggt gtcagccgga aagcgacgtt attgcagaat
     7981 taagccgccg tttcagctgc acgctggagc actggtatgc cgaacagggc tgtgatttct
     8041 gtggctggca gttgtatgag cgcggagagc tcgttgatgt gctgtggggg gaactggaat
     8101 ggtcttcccc gacagatgac gatgagctgc cggaagtcac cggacctgcg tggatagtcg
     8161 acaatgtggc gcattatggc ggatgaagta tgacggagac gggcgggaaa ccgcccgttt
     8221 cttttccgac aaaggatgtc gccgtgctcc tctttttact gcgccggctg acgcggcgcg
     8281 gcataaacgc tgcctgtggt cagtgtcccg cgtccggccg ggcacgggac gggcgctttt
     8341 accgctcccg gctggcatcg gtgacagtgt acgccagccc gtcgcccttt tctgatgaac
     8401 gcccttcaag ccgctttcgc ggcatattct cgccgtcaaa aagacgacgg ctgcgatatt
     8461 ccacggtcgg cctgacccgt taccggacgc ggtgaacagc ccacaggcag cggggaacgg
     8521 gcaccacagg ggtgccctcc cggtgccctc tgtaagagaa ggagtttcgt atgtcccgtt
     8581 ttgtcctcgg taactgcatc gatgttatgg cccgtattcc tgataacgcc attgatttca
     8641 tcctcaccga cccgccgtac ctcgtcggtt tccgtgaccg ttccgggcgc accatcgctg
     8701 gcgataaaac cgatgaatgg ctgcaaccgg cctgtaatga aatgtaccgc gtactcaaaa
     8761 aagatgcgct gatggtcagc ttctatggct ggaaccgcgt cgatcgcttt gtggccgcct
     8821 ggaaaaatgc gggattcagc gtggtcggac acctggtgtt taccaaaaac tacacatcga
     8881 aggccgcata tgtgggctat cgccacgaat gcgcctacat cctggcaaaa ggccgtccac
     8941 gtctgccaca aaacccgctg ccggacgtgc tgggctggaa atattcgggc aaccgtcatc
     9001 acccgacgga aaagcccgtt accagcctgc aaccgctgat tgagagcttc acacacccga
     9061 acgcgattgt gctggacccg tttgcaggca gcggctcaac ctgcgtcgcc gccctccagt
     9121 ccggacgccg gtatatcggt atcgagctgc ttgagcagta tcaccgtgcc ggacagcaac
     9181 gtctggctgc cgtacaacgg gccatgcagc agggggccgc gaatgatgac tggtttatgc
     9241 cggaggctgc gtaaatgaac tatgccggac acgaaaaact tcgcgccgaa gtggcggagg
     9301 tggccaatgc catgtgcgac cttcgggcca ggctgaacga tatggagcat cgctgtcgct
     9361 ttgattccga tgtgcttgtt gaacgcctgg cacgccagac gctgtatcgc gccaatcgcc
     9421 tgttcatgga ggcatatacc gaaattcttg aactggatgc gtgcttcaaa gattaaggag
     9481 gagacgaaat gtacggaaca tgcgaaacgc tctgccgtga gctggcagca aagtatccgg
     9541 gaaacacgcc gctgatgctg cttatctggt ccccggaaga gattcaggca ctggccgacg
     9601 gtatggaaat ttctctgacc gatcatgaaa tcaggactgt cctggcgcac ctggaggaca
     9661 tcccggaaga ccagcggatt gagtccggta tttcttccgc cgctgcgatg gagattatca
     9721 gcaacgtgag cgaaaaccgc ctggtgaccg tctctgctga actgctggcg tccctgattc
     9781 agaccgctga acaggcactg tggaaacgtg aatgggccgc ccgtgataac ggcctcgccg
     9841 tcccggaatc cgtcacccgc cgtcaggcgg tggttaatca ggcccgcagc ctgctgaaaa
     9901 acaacacaca cgaaaacgac tgatgttatc gccgcctccg ggcggcgatt cagggagaac
     9961 gattaatcat gaacgaaaac acaacgctga atgcactgat ttgtcgtcac gcccgtaacc
    10021 tgctgctggc gcagggctgg ccggaagaga cggatgttga ccagcgaaat ccgaactatc
    10081 cgggctggat cagcatttac gtgcggctgg atgcgccccg gctggcgacg ttacttatca
    10141 accgtcacgg cggcgtactg ccgccgctcc tggcctccgc cattcagaga ctgaccggaa
    10201 ccggggcgga actggtactg tccggcagtc agtggcaatc gctgccggta cttccggcag
    10261 acggaacgca ggtgtctttc ccgtatgccg gagaatggct gaccgaagac gaaatcaggg
    10321 ctgttcttga tgcggtgcac gatgcggtac aaagcatctg ttaccaggtg gcagaagatg
    10381 cgcggcgtat ccgtgcggcg ctgaccacca ccggtcagac gttgctgacc cgccagacgc
    10441 gtcgctttcg cctgatcgtg aaggaaagcg atcacccctg ctggctcgat gaagacgacg
    10501 aaaacctgcc cgtggtgctc gatgccatcc tgaaccgggg cgcacgtttt tcggcggtgg
    10561 aaatgtatct ggtcagtgag tgtgtggagc atatcctgtc cagtgggctg gcctgcgatg
    10621 tgctgcgtat acctgatgaa ccgccccgcc ggtggtttga ccgtggtgtt ctgcgggagg
    10681 tggtccggga agcccgggcc gaaatccgca gcatggcgga tgccctggca aaaatcaggg
    10741 gctgatgatg gcggggataa tccccgcctt tttcctgccg gtcccggaca gagcacaaag
    10801 gaagtcgccg ccggctccgc tttacccggc catgcgggca tggccttatg gggtttcagt
    10861 tatgtggcct cagcgccgtg tgcgggctgt gccgtgcctc catcttagcc gggctggcgt
    10921 tgatgcaagg gtacgcttcg ccgctgcggt cacccggtcc ctccttcccg tctgccgtga
    10981 ttttccggtc gttcgcccgc tcaggtttcg cggcctcacc ctgcaactcc cgccagccgt
    11041 gtgcgtcagg ctgcggcttc ccttgcgccc ctgcatcccc gccttatcgc ccggctttta
    11101 tggaggcacg gcaccgcccg gtgtcgcgga atatgtgaac aacggaggaa atcccatgca
    11161 gtatgcgaaa cctgtcactc tgaatgttga agagtgcgac cgtctgtctt ttctgcctta
    11221 cctgtttggc ctggattttc tgtatgccga agcgtctgtg tacgcgctgg cgaaacaaat
    11281 gatgccggaa tatgaaggcg gcttctggca cttcatccgc ctgccggacg gtggcggtta
    11341 catgatgccg gatggcgacc gtttccacat ggtgaacggt gcaaactggt ttgaccgtac
    11401 cgtgagtgct gatgcctgcg gcatcattct gacttccctt gtgattaacc gccagttgtg
    11461 gctgtaccac gacagcggtg atgcaggact gacccagctt taccggatgc gcgatgcgca
    11521 gttgtggcgt cacatcgaat ttcaccctga atgcaacgcg atttacgcgg ctctggactg
    11581 attaacggac ggggcggcaa tgccgcccct gtaaaggaga cgagaaaatg tactgtactg
    11641 ttaaagaaat tatccgcgaa gtgctggata cagacgtgcc ggacagtgaa tgcgtttttg
    11701 ccgtggtgct gacccgtggg gatgtgcgtc acatagccca ggactggaac ctgtcagacg
    11761 atgagctgga aaccgtcatg cagcggctgg acgatgcctt tgagcatggt gcggatgtca
    11821 gcgttgttca cgacgttgtt cgtgagctga tggtagaaaa gcgcgccagc cgtcaggtga
    11881 cagtcccggc ggtgatgctg gaaaaagtga tggcgctggc aggcagtgaa atgaagcgcc
    11941 tgtatgccgt cgggagtgag aacgggggcg acggtgacgc attcgtcagg gaggaacgcg
    12001 aagcaatgga cgttgtgtta caggcgctgg acggggagac gatgtcatga atatcagcac
    12061 agaaacccgc gaaattctgc gtaattacaa agccgtgatt aatgcgcggc gtcgtgaaat
    12121 ggggcagaaa ccgctcacca ccgcgcagat tgttgatgaa atctgcgatt ttgtggcgaa
    12181 tcagcaggct gttttcctgg gcggtcacta catcttacag gacagcagaa acaggtgatg
    12241 cagggcaggc ggtggaggcc gcctgtcagc ccgcgcgaaa acccctggca ggctgacgcc
    12301 tgccggaaat gcggggtgca gcgcctgcgg cgctgccggc ccggctttcc ggtcagaagg
    12361 tagtgtatgg ctgtatgcag ggaacaggtg tgatattccg tgacgctgtc accgtttctg
    12421 gctgtctttt attggtcggg tagcagcggc acattgctgc gccgccgccc cctaagaacg
    12481 gagcgtgcga gtttccccgc actccgctca agcctttata agtgctttcg gtgagcaccg
    12541 gcttccctgc tgtgtaactg tcggtggcag ttcggatgta acaactcgag attggtcagt
    12601 tcgtcactgc cgcccataac tttcctgatc cggtgatgta tattccatcc ggtctggttg
    12661 gttatgagtt gtccgcactg cggacagtgt ttcccctgcc tcttccagag ttgaactact
    12721 gtcttcatct tctttgattt gcctttccag attagatcca gacgacgttc aaagtagatt
    12781 tccattccgg gggtgtatgg tgttgcttcg cctttgattt ttatatggcg ctttatcggt
    12841 gtttccattg cccggaagag tgtcagaatt ttcccttccg gggttatggc tttaaagatc
    12901 cagcgcctgc cttcaaagga gaagtatcgt ccggcgatcc accgcaatcc tttccgggga
    12961 tgccgtctta cacaccatcg ccatatagct cgccagattt gggtgtcgac ataattaaat
    13021 gtttctttcg ccacgacgtg tcggtgatag gtagcccatc cccggatcac cgggtttagc
    13081 tggcctatca gtttccatgc cgggagcgtc ggattgcgtt taattatttc tctgacttta
    13141 cagaggaagt tcttcaggtt tttcttcgac ggtttaatca gcattttacc gttgtacttc
    13201 ctgacgttct gccctaaaaa gtcgaagcct ttatctatat gtgtgatcgc tgttttttct
    13261 tccgacagtt ccagccctcg ttcgcgcatg aaagctgcaa tcagtggtct gacctcattt
    13321 tccagcaatt cacgggagct gcctgaacag atgaaatcat cagcatatct cacataattc
    13381 acctggtgct ttgcactgtg ataaccactt ttcctgaacg tttgcatcag ttccttctcc
    13441 agtccgtcca gagccatgtt ggcgagaaca ggggatatga tgcctccctg cggtgtcccg
    13501 ctgttcgtat ggttaaacac gcctctttcc atgtaacctg ctttcaacca ttttttaagc
    13561 agtctcctgt ccatcggaat atgggaaagc agccagtcat ggctgatgtt atcgaaacac
    13621 cctttaatat cgccctccag tatccatttg ggcgatactt tctggctcat ccgcagaaaa
    13681 atggactcaa tggcgtcatg cgtgctgcgc attggtctga aaccatagct gttatggtca
    13741 gcagtggtct cggatacggg ttccagagcc agcagccaca gtgcctgcat ggcccggtcc
    13801 ctcattgtgg gtatgcccag cggtcgcgtt ttgccgtttg attttgggat atgtattcgc
    13861 cgtagtggct gtggcctgta accacacaga ttcaggctgc atattccttc ccatttctcc
    13921 ttcggtgtgt gccatatttt tccgtcaata ccgggtgtcc ttttaccggt attttcagtt
    13981 acacgcctga cagccacggc tttgccggag aatgagcggg tgaggatgcg ctgtagttct
    14041 ctgacctgcc gccattgttg ctgtcgggtt gcctttgcaa tccgtaactg tagctttcgc
    14101 acccgggcat ggcatgcttt ccagtttatg gtgtgccagc ctgtcgggtg gggtatcgtg
    14161 gacacactga tgtgtgtatt catctcgctg atatcctttt gactgcttta cagcttttca
    14221 tgcaattaaa gaccagacgg aagtctgccc gctttcgcgt cggggaacct gccccgtatc
    14281 ccggttatta cggccgggca ttcgcttttt ccgtcctcct atgcccgcac cgctaacagt
    14341 gttccttgcg gttcacctgc ctgtgaggca gcgatacggg tttaccaggt tccgcttaac
    14401 atacagaggg tcagggcggg ttctgtctct tcaccggggg tctctgcgcc catgtgctcc
    14461 ctgtgcgcga ggtagcaacc ggaccccgta ccttttggtt caggcctgtc agcatctttg
    14521 gcctgttgcc gataacggtg tttatcaaca gttcacatgt gttaaccctt ctgaccagcc
    14581 ttgctctcct gccgcatgat gcttgcagca aggtaaagcc cctcacgggg ctttacccgg
    14641 gctgacgccc ggctctcgtt gtcccgggag cttcagaccc cgccgttgcc ggcaacgcct
    14701 gtcccggtag gctacagcta atgggatagc tggttttatc aatgtaaaac aatacg
//
LOCUS       DAFNBJ010000120        14726 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000120 DAFNBJ010000000
VERSION     DAFNBJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.120"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(343..1539)
                     /gene="hemY"
                     /locus_tag="L2X49_003499"
     CDS             complement(343..1539)
                     /gene="hemY"
                     /locus_tag="L2X49_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBN4798527.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1542..2747)
                     /gene="hemX"
                     /locus_tag="L2X49_003500"
     CDS             complement(1542..2747)
                     /gene="hemX"
                     /locus_tag="L2X49_003500"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBN4798528.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQAD
                     TSAAAPQGE"
     gene            complement(2769..3509)
                     /gene="hemD"
                     /locus_tag="L2X49_003501"
     CDS             complement(2769..3509)
                     /gene="hemD"
                     /locus_tag="L2X49_003501"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBN4798529.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3506..4447)
                     /gene="hemC"
                     /locus_tag="L2X49_003502"
     CDS             complement(3506..4447)
                     /gene="hemC"
                     /locus_tag="L2X49_003502"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBN4798530.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4834..7380
                     /gene="cyaA"
                     /locus_tag="L2X49_003503"
     CDS             4834..7380
                     /gene="cyaA"
                     /locus_tag="L2X49_003503"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBN4798531.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7420..7740)
                     /gene="cyaY"
                     /locus_tag="L2X49_003504"
     CDS             complement(7420..7740)
                     /gene="cyaY"
                     /locus_tag="L2X49_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBN4798532.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7902..8044)
                     /locus_tag="L2X49_003505"
                     /pseudo
     CDS             complement(7902..8044)
                     /locus_tag="L2X49_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8203..8406
                     /locus_tag="L2X49_003506"
     CDS             8203..8406
                     /locus_tag="L2X49_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBN4798533.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8443..9267
                     /gene="dapF"
                     /locus_tag="L2X49_003507"
     CDS             8443..9267
                     /gene="dapF"
                     /locus_tag="L2X49_003507"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBN4798534.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9264..9971
                     /locus_tag="L2X49_003508"
     CDS             9264..9971
                     /locus_tag="L2X49_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBN4798535.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMVLLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            9968..10864
                     /gene="xerC"
                     /locus_tag="L2X49_003509"
     CDS             9968..10864
                     /gene="xerC"
                     /locus_tag="L2X49_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBN4798536.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10864..11580
                     /gene="yigB"
                     /locus_tag="L2X49_003510"
     CDS             10864..11580
                     /gene="yigB"
                     /locus_tag="L2X49_003510"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBN4798537.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11664..13826
                     /gene="uvrD"
                     /locus_tag="L2X49_003511"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11664..13826
                     /gene="uvrD"
                     /locus_tag="L2X49_003511"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBN4798538.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13973..14698)
                     /locus_tag="L2X49_003512"
     CDS             complement(13973..14698)
                     /locus_tag="L2X49_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBN4798539.1"
                     /translation="MNLKRIFLALTLLPLFAVAADDCALSDPMLIVQAYTVNPQTERV
                     KMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLGLYIENGQQKVALN
                     LASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKEIQFAVQSGPMLMENGVINPRIH
                     PNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFSCYAKAKLNVEQLLYLDGTISHMY
                     MKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3336 a         3869 c         4004 g         3517 t
ORIGIN      
        1 tactcaggaa gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt
       61 tcccgattct ctgtggcata ataaacgggt agatgctcat tccatctctt atgttcgcct
      121 tagtgcctca taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac
      181 agatgtcgct tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg
      241 ttgtcataca gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca
      301 tcagaaaggt cccggaggtg cttgcctccg ggtgagaagg aactactgtg gtgggttgtt
      361 ctgcaacgtt aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag
      421 tctgtccagc gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc
      481 cgcgcggaag gcgagcgatg cttcctgcca ttctccgtgc ttcatcagtg actggcccag
      541 tgtgctccac aacagcgggc gatcgccgac gtttttgatc tgctggcgta gcactttttc
      601 cagctgttcc gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata
      661 ctggcgtttc aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat
      721 aagatgttcc gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt
      781 tttccaccag ttacgcaaac cttcgctacc gttatcggca cgagcctgat ccatcaggcc
      841 aatccatgcc tgttgttcca gcattgcgcg atgttcttca tcaccaacat gggctttcgc
      901 cattgatggg ataatatcca gcagcgaact ccatgcacct gtgcggatat acgcctgttc
      961 cgccagacgt aatacttccg gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg
     1021 gcgtgcagca tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg
     1081 aatggtgtca ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc
     1141 atcaccacgt tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttccgc
     1201 gtgatcggca tttttcgcca tcagcttttc aacttgctga taatcgcctt ccgccagttt
     1261 cagcagcgcc tgttcggtct gcttacgggc acggcgacgc ttacgtccga caaaccaccc
     1321 acgggtgtgc gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac
     1381 taccatcgcc agaatcaaaa taatcgccag gcccgtgacg ctggtttcga tattgtagtt
     1441 gtcggtctgg atcagcacat aaccctgatg accggcaatc atcgggccaa ccacgatccc
     1501 cgcaatcagc aacacaaaga gcaataacac ttttagcatg gttattctcc ttgcggcgca
     1561 gctgccgaag tatcagcttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt
     1621 ttagcttccg ttgtccccgc tgccggttgt gccagcaggt tacgcacgcg agtctgcatc
     1681 agtttttcca gcatcgcctg gctttgcagg gtttccggaa gatccatcga gatattttgc
     1741 tggcttaact ggtccacctc gtcgaggaac gctttggtgg tggcatcatc agtatcgtag
     1801 taagcacgta cccaggtgga gacgttctcc agcgcctggc gataagtctc ttgctggtga
     1861 cgcggtacag cttgtgctgc gaccagcagg cgagagcgaa tattttcgcg cagatagata
     1921 tcctgatttg gcgctaacag cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag
     1981 ttgtccataa aattctgcca gcttttttgc agattgatac gccattcgct gatggaactg
     2041 gaaagctctt caccgtcgga atccatcggc gaaccatcgc tgtcattatc ggccagacgc
     2101 aggttatcta cctgatttga aagctgattg agcttaagga tgatgccgtc ataatccacc
     2161 tgcgataccg cagaaaggct ggcgatatca tcggtaattg cccggcgaac ggtaatcaga
     2221 ctcgggtcat tcatatccgc caggctggca tctgcacttt tcagcaacgc tgcagcggtc
     2281 gtgacgtctt gatcgctcca cagcttccgt ccggcgagtt tcaccagaaa atcggcctga
     2341 gccagcagcc aggttttagc atcgctgccg gaaatggtgg cgaccttttg ttggacttca
     2401 tccaactgtt ttgccagcgt ttcttgctga cgattcgcct gcttaagctg tgcagcttgt
     2461 tgcttaataa tgccttccag ctcggctttt tggctctcct gggctttttg caatgccgtc
     2521 agttggttag ccagggcatc gctggtggcg gtctgattga cggcctgttg tttaccccag
     2581 ccatacaaac cgacgcccgc cgccagagca atagcgatag ccaccgcgct gagaatcaat
     2641 gcggtattgt tcttactctt tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc
     2701 tccctggtct cttcaaccac ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt
     2761 tatgagagtt attgtaatgc ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta
     2821 atgtcttgcc agcccagttc ccgggcgagt ttcgccaaac gctcactgac gaccaatagt
     2881 cgacagtgta gtaaccagtg ctcacgatac cattgtggga tcagcgacca gagttgctgc
     2941 aacatttcac cgctggtaac aacgaccgtc gtcacctcgc gggattgcca gcgcatcgct
     3001 tcttctgcac catcgtaatg gattgcgcat cgttgataac attcacaaaa agtgacctca
     3061 gcaccgcgcg ccgtcagggt atccccaatt agctcacgac cgccattgcc acgtaatatc
     3121 agcgcacgtt tgcccgcaat attttgtaat tcaggtaatt gtagcaagac ttcgctgatt
     3181 tcccgatcct gcgggtagag aatcttctgt ccgcttacgg tatgtagtgc cagtgcggtg
     3241 gtgcgtccaa tggcgaaata attaggtagt cggggccatt tacgatcttg ctgatgtagc
     3301 tgtgattggg caaaagcaac cgcgtgttgc gagagggcaa acaacagatc gctctccccc
     3361 agcgctgcca gttgatcagc aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc
     3421 ggaaaatgcc aggccacctg tcccagtgtg cgcagacggc tcactaactc ttctccagcg
     3481 ggagacgggc gggtgaccag gatactcatg ccggggcgtc tccgttatag acttcagcga
     3541 ggatctcgcg cgcgccgtta ttcagtagct cttctgccag cgaaatcccc atttgttcgg
     3601 catcttgcgg cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga
     3661 ccagcgcacg tagccagatt tcgccatcaa taagctcggc gtagctacca attggcacct
     3721 gacatccgcc ttcgagacgg gtattcatgg cgcgttctgc ggtaacgcgc agtgcagttt
     3781 cgtggtgatt cagcgcggca agcagctcgc gagtgcgtgc atcatcaagg cggcattcaa
     3841 tacccaccgc accttgccct accgccggaa gagaaatctc gggcggtaac gcggcgcgaa
     3901 tgcgggactc cagacctaaa cgttttagtc cggctacggc aagaatgatg gcatcgtatt
     3961 cgccgttatc cagtttactc aggcgagtgc cgacgttgcc gcgcagggag cggataatca
     4021 gatccgggcg gcgttcagcc agttggcact ggcgacgtaa actggacgtc ccgacgatac
     4081 tgcctgccgg taacgcatcc agactgtcat agttattgga cacaaaggca tcgcgaggat
     4141 cttcacgctc acaaatagtg accagtccca gaccttgcgg aaattcaacc ggcacatctt
     4201 tcattgagtg tacggcgata tcggcgcgat tttcgaggag cgcgacttcc agctctttta
     4261 caaataagcc ttttccacct actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg
     4321 tcaccatcgg taccagttca acgaccaggc ccggatggct cgccatcaac ttgtctttga
     4381 catagtgtgc ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt
     4441 ctaacatgct tgttaccgtc attatcatcc gtggttcatc ctaacatcct tgccagagtg
     4501 atgtcagtgt tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt
     4561 ggatcctgac aggcgtttca cgccgtcgta ataaggaatt tacagagaat aaacggtgct
     4621 acacttgtat gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa attgatcacg
     4681 ttttagacca ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt
     4741 acctttgaca tacgaaatat cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac
     4801 agtcatgacg ggtagcaaat caggcgatac gtcttgtacc tctatattga gactctgaaa
     4861 cagagactgg atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct
     4921 gcattccaac aggtctacag tctactgccg acattgttgc actatcacca tccgctaatg
     4981 ccgggttacc ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact
     5041 caacgccact acctgaacga gcttgaactg tatcgtggaa tgtcagtaca ggacccgccg
     5101 aaaggtgagc ttccaattac tggtgtatac accatgggca gcacctcgtc cgtagggcaa
     5161 agttgttcct ctgacctgga tatctgggtc tgtcatcaat cctggctcga tagcgaagag
     5221 cgccagttgc tacaacgtaa atgtagtctg ctggaaagct gggccgcctc gctgggtgtg
     5281 gaagtcagct tcttcctgat tgatgaaaac cgcttccgtc ataatgaaag cggcagcctg
     5341 gggggcgaag attgtggctc cacccagcat atactgctgc ttgacgaatt ttatcgtacc
     5401 gccgtgcgtc tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga cgaagaagaa
     5461 cattacgacg actatgtgat gacgctttac gcgcagggcg tgctgacgcc aaatgaatgg
     5521 ctggatctcg gtggcttaag ctcgctttct gctgaagagt actttggtgc cagcctttgg
     5581 cagctctaca agagtatcga ttccccatac aaagcggtgc tgaaaacact gctgctggaa
     5641 gcctattcct gggaataccc gaacccacgt ctgctggcga aagatatcaa acagcgtttg
     5701 cacgacggcg agattgtatc gtttggtctc gatccatact gcatgatgct ggagcgtgtt
     5761 actgaatacc tgacggcgat tgaagatttt acccgtctgg atttagtacg tcgctgcttc
     5821 tatttaaaag tgtgcgaaaa gctcagccgt gaacgcgcct gcgtaggctg gcgtcgcgca
     5881 gtgttgagcc agttagtgag cgagtggggt tgggacgaag ctcgtctggc aatgctcgat
     5941 aaccgcgcta actggaagat tgatcaggtg cgtgaggcgc acaacgagtt gctcgacgcg
     6001 atgatgcaga gctaccgtaa tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc
     6061 gccagtccgc aggatatcgg cgtgctgacg cgtaagctgt atgccgcgtt tgaagcatta
     6121 ccaggtaaag tgacgctggt aaacccgcag atttcacccg atctctcgga accgaatctg
     6181 acctttattt atgtgccgcc gggccgtgct aaccgttcag gttggtatct gtataaccgc
     6241 gcgccaaata ttgagtcgat catcagccat cagccgctgg aatataaccg ttacctgaat
     6301 aaactggtgg cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg tttgtatatt
     6361 aaaggtaacg gcattgtcga tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac
     6421 catttcccgc tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatc
     6481 cgccatctgg cgattatcgt taacctggaa tatgacccga cagcggcgtt ccgcaatcag
     6541 gtggtgcatt tcgatttccg taagctggat gtcttcagct ttggcgagaa tcaaaattgc
     6601 ctggtaggta gcgttgacct gctgtaccgc aactcgtgga acgaagtgcg tacgctgcat
     6661 ttcaacggcg agcaatcgat gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag
     6721 gacgccgcac cgccagatag cgtggaagtc ttctgttata gccagcatct gcgcggctta
     6781 attcgtactc gcgtgcagca actggtttct gagtgtattg aattgcgtct ttccagcacc
     6841 cgccaggaaa ccgggcgttt caaggcgctg cgcgtttctg gtcaaacctg ggggttgttc
     6901 ttcgaacgcc tgaatgtatc ggtacagaaa ctggaaaacg ccatcgagtt ttatggcgcg
     6961 atttcgcata acaaactgca cggcctgtca gtgcaggttg aaaccaatca cgtcaaatta
     7021 ccggcggtgg tggacggctt tgccagcgaa gggatcatcc agttcttttt cgaagaaacg
     7081 caagacgaga atggctttaa tatctacatt ctcgacgaaa gcaaccgggt tgaggtgtat
     7141 caccactgcg aaggcagcaa agaggagctg gtacgtgacg tcagtcgctt ctactcgtca
     7201 tcgcatgacc gttttaccta cggctcaagc ttcatcaact tcaacctgcc gcagttctat
     7261 cagattgtga aggttgatgg tcgtgaacag gtgattccgt tccgcacaaa atctatcggt
     7321 aacatgccgc ctgccaatca ggatcacgat acgccgctat tacagcagta tttttcgtga
     7381 tgaacgtgcc ggaaagcgag gcttatccgg catgcaatct tagcggaaac tgactgtttc
     7441 acccgcctgc tgcgtcgccg cctgttccag caaatcccag aaggtttcgc cgctgcgatc
     7501 gcaaatccac tcatcgcctt tcagatcaaa atggtagccg ccttgtttgg ttgccagcca
     7561 tacctggtgc agcggttcct gtcggttgat aatgattttg ctgccgtttt caaaggtaat
     7621 ggtcagtacg ccgccgttga tttcgcagtc gatatcgctg tcgccatccc agtcgtccag
     7681 gcgttcttca atggtcagcc agagttgatc agccaggcga tgaaattcac tgtcgttcat
     7741 tgttgtatcc tgtttttaag tgatggcggc agtatagcgg catggggtca gggcttcaaa
     7801 gtttgcacct ctgcggctgc gttccggcac gattcatccg tcaccggaat aatgatgtct
     7861 cagtgtagcg aaagatttgt ctcttcatta gggcgcagtt acaccacgcc tttccctgtt
     7921 tctggtaaac attatgatca ggtttaccga gcgagcatcc tcacgctgac ggaactaaaa
     7981 aagccaacaa acaaaccaca ttgcgatagt gcataaagcc atcctggcgc gaggtgttga
     8041 tcacgaaact gccagcagaa cataaatccc cacgagtaag cgttatactc gcagcatttc
     8101 ctcacttttc agacttcata aagagtcgct aaatgcttgc ttttacgcct tctcctgcga
     8161 tgatagaaag cagaaagcga tgaactttac aggcaatcca taatgaaaaa cgtgtttaag
     8221 gcactcactg tattacttac tctcttcagc ctgacgggct gcggtctgaa aggtccgctc
     8281 tatttcccgc ctgcagataa aaatgcaccg ccgccgacca aaccggtaga gacgcaaacg
     8341 caatccacgg tgccggataa aaacgatcgc gccactggcg atggtccatc ccaggtgaat
     8401 tactaaaagt cagtttctgt acccgcgtga ttggagtaaa tgatgcagtt ctcgaaaatg
     8461 catggccttg gcaacgattt tatggtcgtc gacgcggtaa cgcagaatgt ctttttttca
     8521 ccggagctga ttcgtcgcct ggcggatcgg cacctggggg tagggtttga ccagctgctg
     8581 gtggttgagc cgccgtatga tcctgaactg gattttcact atcgcatttt caatgctgat
     8641 ggcagtgaag tggcgcagtg cggcaacggt gcgcgctgct ttgcccgttt tgtgcgcctg
     8701 aaagggctta ccaataagcg cgatatccgc gtcagcaccg ccaacgggcg gatggttctg
     8761 accgtcaccg atgatgattt ggtccgcgta aatatgggcg aacccaactt cgaaccttcc
     8821 gctgtgccgt ttcgcgctaa caaagcggaa aagacctata ttatgcgcgc cgccgagcag
     8881 acaatcttat gcggcgtggt gtcgatggga aatccgcatt gcgtgattca ggtcgatgat
     8941 gtcgataccg cggcggtaga aacgcttggt cctgttctgg aaagccacga gcgttttccg
     9001 gagcgagcca atatcggttt tatgcaagtg gttaagcgcg agcatattcg tttacgcgtt
     9061 tatgagcgtg gggcaggaga aacccaggcc tgcggcagcg gcgcgtgtgc ggcggttgca
     9121 gtagggattc agcaaggttt gctggccgaa gaagtacgcg tggaactccc cggcggtcgt
     9181 cttgatatcg cctggaaagg tccgggtcac ccgttatata tgactggccc ggcggtacat
     9241 gtctacgacg gatttattca tctatgaagc aaccagggga agaactgcag gaaacactca
     9301 cggagcttga tgaccgggcg gttgtcgatt atctgattaa aaatcctgag ttttttatcc
     9361 gcaatgcgcg cgcagtagaa gcgatacgtg tgccgcatcc ggtacgcggc accgtttcgt
     9421 tggtcgagtg gcacatggcc cgcgcacgta atcatattca tgttctggaa gagaacatgg
     9481 tgctgttgat ggaacaggct atcgccaacg aaggcctgtt ttatcgccta ctctacctgc
     9541 agcgcagtct caccgccgcc agcagtctcg acgatatgct gatgcgcttt caccgctggg
     9601 cgcgcgatct cggcctggca ggtgcgagtc tgcgcctgtt tccggatcgc tggcgcttag
     9661 gtgcgccgtc gaaccacact catctggcat taagccgtca gtctttcgaa ccgctgcgta
     9721 ttcagcgttt ggggcaggaa cagcactatc ttgggccgct taacggacca gagctgctgg
     9781 tggtgctacc ggaagcgaaa gcggtgggat cggtggcgat gtcgatgctg ggaagcgatg
     9841 ctgatttggg tgtcgtgctg tttaccagtc gtgatgccag tcactatcaa caagggcagg
     9901 gaacgcagtt acttcatgaa attgcgctga tgttgccgga gcttctggag cgttggattg
     9961 aacgcgtatg accgatttac acaccgatgt agaacgctac ctacgttatc tgagcgtgga
    10021 gcgtcagctt agcccgataa ccctacttaa ctaccagcgt cagcttgagg cgatcatcaa
    10081 ttttgccagc gaaaacggcc tgcaaagctg gcaacaatgc gatgcagcga tggtacgcaa
    10141 ttttgctgta cgcagtcgcc gtaaagggct gggagcagca agtctggcgt tacggctttc
    10201 tgcgctacgt agcttttttg actggctggt cagccagaac gaactcaaag ctaacccggc
    10261 gaaaggagtt tcggcaccga aagcgccgcg tcatctgccg aaaaacatcg acgtcgacga
    10321 tatgaatcgg ctgctggata ttgatatcaa cgatcccctc gctgtacgcg accgtgcaat
    10381 gctggaagtg atgtacggcg cgggtctgcg tctttccgag ctggtggggc tggatatcaa
    10441 acacctcgac ctggagtctg gtgaagtgtg ggtaatgggg aaaggcagca aagagcgccg
    10501 cctgccgatt ggtcgcaacg ctgtggcgtg gattgagcac tggcttgatt tgcgcgacct
    10561 gtttggtagc gaagacgacg cgctttttct gtcgaaactg ggcaagcgta tctccgcgcg
    10621 taatgtgcag aaacgctttg ccgaatgggg cataaaacaa gggctgaata atcacgttca
    10681 cccgcataaa ttacgtcact cgttcgctac gcatatgctg gagtcgagcg gcgatcttcg
    10741 tggtgtgcag gagctgctgg gtcatgccaa cctctccacc acgcaaatct atactcatct
    10801 tgattttcaa caccttgcct cggtgtacga tgcggcgcat ccacgcgcca aacgggggaa
    10861 ataatgcgtt tttaccggcc tttggggcgc atctcggcgc tcacctttga cctggatgat
    10921 accctttacg ataaccgtcc ggtgattttg cgcaccgagc gagaggcgct tacctttgtg
    10981 caaaattatc atccggcgct gcgcagcttc cagaatgaag atctgcaacg cctgcgccag
    11041 gcggtacggg aagcggaacc cgagatttat cacgacgtga cgcgctggcg ttttcgttcg
    11101 attgaacaag cgatgctcga cgccgggctg agtgccgaag aagccagtgc aggcgcacac
    11161 gcagcaatga tcaactttgc caaatggcgc agccgaatcg acgtcccgca gcaaactcac
    11221 gacaccttaa aacagctggc gaagaaatgg ccgctggtgg cgatcaccaa cggtaacgcc
    11281 cagccggagc tgtttggttt gggggattat tttgagtttg tgctgcgcgc tggcccgcac
    11341 gggcgctcaa aaccgttcag cgatatgtac tttttggctg cggaaaaact caacgtgccg
    11401 atcggcgaga tcttacatgt tggggacgat ctcaccactg acgtgggtgg ggcaattcgc
    11461 agcggaatgc aggcttgttg gatcagaccg gaaaatggcg atctgatgca aacctgggac
    11521 agccgtttac tgccgcatct ggaaatttcc cggttggcat ctctgacctc gctgatataa
    11581 tcagcaaatc tgtatatata cccagctttt tggcggaggg cgttgcgctt ctccgcccaa
    11641 cctattttta cgcggcggtg ccaatggacg tttcttacct gctcgacagc cttaatgaca
    11701 aacagcgcga agcggtggcc gcgccacgca gcaaccttct ggtgctggcg ggcgcgggca
    11761 gtggtaagac gcgcgtactg gtgcatcgta tcgcctggtt gatgagcgtg gaaaactgct
    11821 cgccatactc gattatggcg gtgacgttta ccaacaaagc ggcggcggaa atgcgtcatc
    11881 gtatcgggca actgatgggc accagccagg gcggcatgtg ggtcggcacc ttccacgggc
    11941 tggcgcaccg tttgctgcgt gcgcaccata tggacgccaa tctaccgcag gatttccaga
    12001 tcctcgacag tgaagaccag ctacgcctgc ttaagcgtct gatcaaagcc atgaacctcg
    12061 acgagaagca gtggccgccg cgccaggcaa tgtggtacat caacagccag aaagatgaag
    12121 gcctgcgtcc gcatcatatt caaagctacg gtaatccggt ggagcagacc tggcagaagg
    12181 tgtatcaggc gtatcaggaa gcgtgcgatc gtgcgggcct ggtggacttc gccgagctgc
    12241 tgctgcgcgc tcacgagttg tggcttaaca agccgcatat cctgcaacac taccgcgaac
    12301 gttttaccaa catcctggtg gacgaattcc aggataccaa caacattcag tacgcgtgga
    12361 tccgcctgct ggcgggcgat accggcaaag tgatgatcgt tggcgatgac gaccagtcaa
    12421 tctacggctg gcgcggggcg caggtagaga atattcagcg tttccttaat gatttccccg
    12481 gtgccgaaac tattcgtctg gagcaaaact accgctctac cagcaatatt ctgagcgcgg
    12541 ctaacgccct gattgaaaat aacaatgggc gcttaggtaa aaaattgtgg accgatggcg
    12601 cggacggtga gccgatttct ctttattgcg cctttaacga actcgacgaa gcgcgttttg
    12661 tggttaaccg catcaaaacc tggcaggata acggcggagc gcttgccgag tgcgccattc
    12721 tctaccgcag taacgcccag tcgcgcgtgc tggaagaagc actgttgcag gcgagtatgc
    12781 cgtaccgtat ttacggcggg atgcgcttct tcgaacgcca ggaaatcaaa gatgcgctct
    12841 cgtatctgcg cctgattgcc aaccgcaacg acgacgcggc ctttgagcgc gtagtgaata
    12901 caccaacgcg gggtattggt gaccggacgc tggacgtggt acgtcagaca tcgcgcgatc
    12961 gccagttaac gctgtggcag gcgtgccgtg aactgttgca ggaaaaagcg ctcgccggac
    13021 gtgcagccag cgccttgcag cggtttatgg aactgatcga cgccttagcg caagaaactg
    13081 ccgatatgcc gctacatgta cagactgacc gggtcattaa agactccggc ctgcgcacca
    13141 tgtacgagca ggagaagggc gaaaaaggtc agacgcgtat cgaaaacttg gaggaactgg
    13201 tgacggcaac gcgccagttc agctacaacg aagaagatga agatttaatg ccgttgcagg
    13261 cgtttctctc ccatgcggcg ctggaagcgg gcgaagggca ggcggatacc tggcaggacg
    13321 cggtgcagtt gatgacgcta cactcggcga aaggtctgga gtttccgcag gtgtttattg
    13381 tcggtatgga agagggcatg ttcccaagcc agatgtcgct ggatgaaggc gggcgtctgg
    13441 aagaagaacg ccgtctggcc tacgttggcg taacccgcgc gatgcagaaa ctgacgttga
    13501 cctacgcgga aactcgccgt ctgtatggca aagaggttta ccatcgcccg tcgcgcttta
    13561 tcggcgagct gccggaagag tgtgtggaag aggtgcgcct gcgcgctacg gtaagtcgtc
    13621 cggtcagcca tcaacggatg gggacgccga tggtcgagaa cgacagcggc tacaagctcg
    13681 gtcagcgtgt acgccacgct aagtttggtg aaggcaccat cgtcaatatg gaaggcagcg
    13741 gtgaacatag ccgtttgcag gtggcgttcc agggccaggg aatcaaatgg ctggtggcgg
    13801 cttacgcccg gctggagacg gtgtaacgtt gccggatgcg gtgctgcgca ccttatttgg
    13861 cctaaaaaat cattcagatt caataaattg caacgtcatg taggccggat aaggcgttta
    13921 cgccgcatcc ggcatcagca gcatatttag tatctgacac aaaactatca ttctaacctt
    13981 tccgctcgac ggaaatcatg gtgacaaaag gatagcgttg ccacgggatt gccccgcctt
    14041 tcatatacat atgtgaaatc gtgccatcaa gataaagcag ctgctcaacg ttcagtttcg
    14101 ctttggcata acaggaaaaa tcataaaaat ttgttgcctg ctggctcaac aaaaacacgg
    14161 cattcccatg tttattaatc ccaacaccgt tacgaatttt acgtgaggcg acgttgggat
    14221 gaatacgcgg attaattaca ccgttttcca tcaacattgg ccctgactgc accgcaaact
    14281 gaatctcttt actggcttta aaggcatcca gacgaacgat gccgacttta tctcccgcga
    14341 cataaaacac gccgccagga cggataaaga aattcccttc acctgaagcg agatttaacg
    14401 ccaccttctg ctgaccgttt tcgatgtaca aaccgagcgg cgcatagctt tcatcataga
    14461 tgccgccgtt catcgccatc tgcacctgac cctgactatt catatccacc agcagagcat
    14521 gtaaagttcc ccacgcttcg ccattggctt tttgccagta cattttcacc cgctctgttt
    14581 gaggattaac ggtatacgcc tgtacgatca gcatcggatc tgagagtgcg caatcatcag
    14641 cggcaacagc aaacaaggga agtaaggtga gggcgagaaa aatccgtttg agattcaagg
    14701 tgatcattcc tttaccaatg agtagc
//
LOCUS       DAFNBJ010000121        14618 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000121 DAFNBJ010000000
VERSION     DAFNBJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.121"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(177..389)
                     /gene="rpmE"
                     /locus_tag="L2X49_003513"
     CDS             complement(177..389)
                     /gene="rpmE"
                     /locus_tag="L2X49_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBN4798540.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            592..2790
                     /gene="priA"
                     /locus_tag="L2X49_003514"
     CDS             592..2790
                     /gene="priA"
                     /locus_tag="L2X49_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBN4798541.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQVGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2946..3971
                     /gene="cytR"
                     /locus_tag="L2X49_003515"
     CDS             2946..3971
                     /gene="cytR"
                     /locus_tag="L2X49_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBN4798542.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4063..5022
                     /gene="ftsN"
                     /locus_tag="L2X49_003516"
     CDS             4063..5022
                     /gene="ftsN"
                     /locus_tag="L2X49_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBN4798543.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5115..5645
                     /gene="hslV"
                     /locus_tag="L2X49_003517"
     CDS             5115..5645
                     /gene="hslV"
                     /locus_tag="L2X49_003517"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBN4798544.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5655..6986
                     /gene="hslU"
                     /locus_tag="L2X49_003518"
     CDS             5655..6986
                     /gene="hslU"
                     /locus_tag="L2X49_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBN4798545.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7053..7979
                     /gene="menA"
                     /locus_tag="L2X49_003519"
     CDS             7053..7979
                     /gene="menA"
                     /locus_tag="L2X49_003519"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBN4798546.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8072..8557
                     /gene="rraA"
                     /locus_tag="L2X49_003520"
     CDS             8072..8557
                     /gene="rraA"
                     /locus_tag="L2X49_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBN4798547.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8642..8887)
                     /gene="zapB"
                     /locus_tag="L2X49_003521"
     CDS             complement(8642..8887)
                     /gene="zapB"
                     /locus_tag="L2X49_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBN4798548.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9313..10158
                     /gene="glpF"
                     /locus_tag="L2X49_003522"
     CDS             9313..10158
                     /gene="glpF"
                     /locus_tag="L2X49_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HBN4798549.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10181..11689
                     /gene="glpK"
                     /locus_tag="L2X49_003523"
     CDS             10181..11689
                     /gene="glpK"
                     /locus_tag="L2X49_003523"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBN4798550.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHALDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11822..12832
                     /gene="glpX"
                     /locus_tag="L2X49_003524"
     CDS             11822..12832
                     /gene="glpX"
                     /locus_tag="L2X49_003524"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBN4798551.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12929..13675
                     /gene="fpr"
                     /locus_tag="L2X49_003525"
     CDS             12929..13675
                     /gene="fpr"
                     /locus_tag="L2X49_003525"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBN4798552.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13680..14108)
                     /gene="uspD"
                     /locus_tag="L2X49_003526"
     CDS             complement(13680..14108)
                     /gene="uspD"
                     /locus_tag="L2X49_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBN4798553.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14135..14434)
                     /locus_tag="L2X49_003527"
     CDS             complement(14135..14434)
                     /locus_tag="L2X49_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBN4798554.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
BASE COUNT         3527 a         3751 c         3923 g         3417 t
ORIGIN      
        1 gccggttttc cgctcatcgt aaaatccttt tgaaataaat aagaaccaga agcctagcgc
       61 cagtcaacaa caggaaatga taatgagtta acaccccgga atttttcaga atttataaaa
      121 ggcaaattac aggcacaaaa aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat
      181 ttgctgcccg ggatgttgaa acgcttgttg aagcggtcaa cacggccacc ggtagcaaca
      241 tcacgctgtt tgccagtgaa gaacgggtgg cacttgctgc acacgtcgag gttcaggtca
      301 tgaccaacgg tggagcggat tttcattacg ttaccgcaag agcagctagc agtaatttct
      361 tcgtatttcg ggtgaatatc ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc
      421 ttccagccct aacgccagac accacgcgat gttaaaagta tagcttcaat acgatcattt
      481 cgtacgaagg cgcgaaatca tacagaaatt aaccagcgta tgcaaactga tccgcactct
      541 tctacggcaa tgtgtatact aacccaccga atttcaagtc aggatgatgc tatgcccgtt
      601 gcccacgttg ccttgcccgt tccgcttcct cgtacctttg actatctgct gccagaaggc
      661 atgacggtta aagctgggtg tcgcgtgcgc gtgccgtttg gcaaacagca ggagcgcatc
      721 gggattgtgg tatcagttag cgatgccagc gaactgccgc tcaatgagct aaaagcggta
      781 gtcgaagtgc tggatagtga gccggtgttt actcactccg tctggcgatt gctgctatgg
      841 gcggcagatt actatcatca tccgattggc gatgtgctgt ttcatgcctt gccgatttta
      901 ctacgccagg ggcggcctgc ggcgaacgcg ccgatgtggt actggtttgc cactgaacaa
      961 ggtcaggcgg tggatctgaa cagcctgaaa cgctcaccca agcaacaaca ggcgctggca
     1021 gcgttacggc aaggcaaaat ctggcgcgac caggttgcca cgctcgaatt taatgatgcc
     1081 gcgttgcagg cgctgcgcaa aaaaggtctg tgtgatttag caagtgaaac accagagttt
     1141 agcgactggc gaacgaacta tgccgtttct ggtgagcggt tgcgattgaa taccgaacag
     1201 gccaccgccg ttggcgcaat tcatagtgcg gcggatacct tctctgcctg gctgctggcg
     1261 ggcgttaccg gttccggtaa aacggaggtt tatctcagtg tactggaaaa cgtgctcgct
     1321 cagggcaaac aggcgctggt gatggtgccg gaaatcggcc tgacaccgca aactatcgcc
     1381 cgttttcgtg aacgttttaa tgcccccgta gaagttctgc attccggctt gaacgacagc
     1441 gagcgtcttt cggcgtggtt gaaagcgaaa aatggtgagg cggcgattgt gatcggcacc
     1501 cgctccgcgc tgtttacgcc gtttaaaaat ctcggtgtga ttgtcattga tgaagagcac
     1561 gacagttcct acaagcagca ggaaggctgg cgctatcatg cccgcgacct ggcggtttat
     1621 cgtgcgcaca gcgagcaaat cccgattatt cttggctccg caacacccgc actggaaacg
     1681 ttatgcaacg tccagcagaa aaaataccgc ctgctgcgcc tgacgcgtcg ggcggggaat
     1741 gcgcgtccgg caattcaaca cgtgctggat ttaaaaggtc agaaggtgca ggtggggctg
     1801 gctccggcgt taatcacccg tatgcgccag catttacagg ccgacaacca ggttatcctc
     1861 tttcttaacc gccgtggctt tgcgcctgcc ttgctgtgcc acgactgcgg ctggattgcc
     1921 gaatgtccac gttgcgatca ctactacacg ctgcatcagg cgcaacagca tttgcgctgt
     1981 caccactgtg acagccaacg cccggtgccg cgccagtgcc cttcctgcgg ttccacgcac
     2041 ctagtacccg tggggctggg caccgaacag cttgaacaga cgcttgcgcc gatgttcccc
     2101 ggcgtgccca tttcacgtat cgaccgcgat accaccagcc gcaaaggggc gctggaacag
     2161 caactggcgg aagtgcatcg cggcggcgcg aggatcttga ttggcacgca aatgctggcg
     2221 aaaggtcacc actttccgga tgtgacgctg gtggcgttac tggatgtgga cggcgcgctg
     2281 ttttctgccg attttcgctc ggcagaacgt ttcgcccagc tttacaccca ggtcgccggt
     2341 cgtgccgggc gtgcgggtaa acagggcgaa gtggtgctgc aaacgcacca tccggaacat
     2401 cctctgttgc aaacgttgct ctataaaggc tacgacgcct ttgccgaaca ggcgctggct
     2461 gagcgacgca tgatgcagct gccgccgtgg accagccatg tgattgtgcg tgcggaagat
     2521 cataacaatc agcatgcgcc gctgttcctg caacaactgc gtaatctgat cctttccagc
     2581 ccgctggcag acgacaaact gtgggttctc ggcccggttc cggctctggc acctaagcgt
     2641 ggtggccgct ggcgctggca gatcttgttg cagcaccctt cccgcgtgcg cttgcaacac
     2701 atcattagtg gtacgctggc gcttattaat acaataccgg attcccgcaa ggtgaaatgg
     2761 gtgttggatg tcgatcccat cgaaggctaa tgcgcaaacg ttgcgaggcg gatcgaaaaa
     2821 ttcaatattc atcacacttt tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta
     2881 aaaatgttgc cgatgtgaag taaacatgga tgtagtacgc ctgacgtgcc aggcgaggag
     2941 tgagtgtgaa agcgaagaag caggaaactg ccgcgaccat gaaagacgtt gccctcaagg
     3001 caaaagtctc tacagcgact gtctcccgag cattaatgaa tcccgataaa gtctcccagg
     3061 ccacccgtaa tcgggttgaa aaagcggccc gggaagtggg ttatttaccg caacctatgg
     3121 ggcgcaacgt caagcgtaat gaatcccgca ccattctggt gattgtcccg gatatctgcg
     3181 atcccttctt tagcgaaatt attcgcggta tcgaagttac ggcggcaaat cacggatatc
     3241 tggtgctgat tggcgactgt gcgcatcaaa atcagcagga aaaaaccttt atcgatttga
     3301 tcatcaccaa gcaaattgat ggcatgttgc tgctgggctc aaggctgccg tttgatgcca
     3361 gcattgagga acagcgtaat ctgccgccga tggtgatggc gaacgaattt gcaccggaac
     3421 tggagctgcc taccgttcat atcgacaatc tgaccgccgc atttgatgca gtaaattatt
     3481 tatatgagca agggcataaa cggattggct gtatagccgg tcccgaagag atgccactgt
     3541 gtcactaccg cctgcaaggc tatgttcagg cgctgcgtcg ctgcggcatt atggttgatc
     3601 cgcaatacat cgcccgtggc gacttcacct tcgaagccgg aagcaaagcg atgcagcagc
     3661 tgcttgatct tccacaaccg cctactgctg tcttctgcca tagcgatgtg atggcgctcg
     3721 gcgcactttc tcaggcaaaa cgccaggggc tgaaagtccc ggaagacctt tccataatcg
     3781 gttttgataa catcgacctg acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc
     3841 cgcgttacga aatcggtcgg gaagctatgc tgttattgct tgatcaaatg caggggcaac
     3901 acgttggcag tggctctcgt ttaatggact gcgaacttat catccgggga tcaacacgcg
     3961 cgttacctta aagtaaacct ttaagactgc cgtgactggt caaaggcccg ccgcttaagt
     4021 aacatggcgg gctgacgaac gaataaatac agcgaaacga tagtggcaca acgagattat
     4081 gtacgccgca gccaaccggc accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa
     4141 cgaaatctgc ctgcggtttc tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc
     4201 tttatcggtg gtctgtactt cattacgcat cacaagaaag aagagtccga gacgctgcaa
     4261 agccagaaag tgaccggaaa cggactacca ccaaaaccag aagaacgctg gcgctacatc
     4321 aaagagctgg aaagtcgcca gccgggtgtg cgtgcgccca cagaaccttc tgctggtggt
     4381 gaagtgaaaa cgccggagca actgacacca gaacaacgtc agcttcttga acaaatgcag
     4441 gctgatatgc gccagcagcc aacgcagctg gttgaggttc cgtggaatga acagacgccg
     4501 gaacagcgtc agcaaacgct acagcgccaa cgtcaggcgc agcaattagc ggaacagcaa
     4561 cgcctggctc agcagtcgcg tacgactgaa caaagctggc agcagcagac gcgtacgtct
     4621 caagccgcgc cggtacaggc gcaaccgcgc cagtctaaac cggcttctac tcagcagccg
     4681 taccaggatc tgctgcaaac ccctgcgcac acaaccgcgc agtcgaagcc gcagcaagct
     4741 gcgccggtta ctcgtgccgc tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc
     4801 cgctggatgg tgcagtgcgg ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct
     4861 cagctggcgt tcgaaggatt tgactcgaaa atcaccacca acaatggctg gaatcgtgtg
     4921 gtcattggcc cggtgaaagg caaagagaac gcagacagca ccctcaatcg gttgaagatg
     4981 gcgggtcata caaactgcat tcggctcgcc gccgggggtt gaaaccctca aaatcccccc
     5041 catctataat tgcattatgc cccgtacttt tgtacggggt ttgtactctg tattcgtaac
     5101 caaggggtca gctcgtgaca actatagtaa gcgtacgccg taacggccat gtggtcatcg
     5161 ctggtgatgg tcaggccacg ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc
     5221 gccgtctgta caacgacaaa gtcatcgcgg gctttgcggg cggtactgcg gatgctttta
     5281 cgctgttcga actgtttgaa cgtaaactgg aaatgcatca gggccatctg gttaaagccg
     5341 ccgttgagct ggcaaaagac tggcgtaccg atcgcatgct gcgcaaactt gaagcactgc
     5401 tggcagtcgc ggatgaaact gcatcgctta tcatcaccgg taacggtgac gtggtgcagc
     5461 cagaaaacga tcttattgct atcggctccg gcggccctta cgcccaggct gcggcgcgcg
     5521 cgctgttaga aaacactgaa cttagcgccc gtgaaattgc tgaaaaggcg ttggatattg
     5581 caggcgacat ttgcatctat accaaccatt tccacaccat cgaagaatta agctacaaag
     5641 cgtaaggatc tcccatgtct gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc
     5701 acatcatcgg ccaggacaac gccaagcgtt ctgtggcgat tgctctgcgt aaccgctggc
     5761 gtcgcatgca gctcaacgaa gagctgcgcc atgaagtgac cccgaaaaat atcctgatga
     5821 tcggcccgac cggtgtcggt aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg
     5881 cgccgttcat caaagttgaa gcgaccaaat tcaccgaagt gggctacgtc ggtaaggaag
     5941 tggattctat tattcgcgat ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta
     6001 tcgagaaaaa ccgttatcgc gctgaagaac tggcagaaga acgtattctc gacgtgctga
     6061 tcccacctgc taaaaacaac tggggacaga ccgaacagca gcaggaaccg tccgctgctc
     6121 gtcaggcatt ccgcaaaaaa ctgcgtgaag gccagcttga tgacaaagaa atcgagatcg
     6181 atcttgccgc agcaccgatg ggcgttgaaa ttatggctcc tccaggcatg gaagagatga
     6241 ccagccagct gcagtccatg ttccagaacc tgggcggcca gaagcaaaaa gcgcgtaagc
     6301 tgaaaatcaa agacgccatg aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc
     6361 cggaagagct gaagcaagac gctatcgacg ctgttgagca gcacgggatc gtgtttatcg
     6421 acgaaatcga caaaatctgt aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag
     6481 gcgttcagcg tgacctgctg ccgctggtag aaggttgcac cgtttccacc aaacacggga
     6541 tggtcaaaac tgaccacatt ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt
     6601 ctgacctaat cccggaactg caaggtcgtc tgccaatccg cgttgaactg caggcactga
     6661 ccaccagcga cttcgagcgt attctgaccg agccgaatgc ctctatcacc gtgcagtaca
     6721 aagcactgat ggcgactgaa ggcgtaaata tcgagtttac cgactccggt attaaacgca
     6781 tcgcggaagc ggcatggcag gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac
     6841 acactgttct ggagcgttta atggaagaga tttcctacga cgccagcgat ttaagcggtc
     6901 aaaatatcac tattgacgca gattatgtga gcaaacatct ggatgcgttg gtggcagatg
     6961 aagatctgag ccgttttatc ctataatcgc gttcaatcat tttcatcatt gtttgatggg
     7021 gctgaaaggc cccattttta ttggcgcgta ttatgactga acaacaaatt agccgaactc
     7081 aggcgtggct ggaaagttta cgacctaaaa ccctccccct cgcctttgct gcaattatcg
     7141 tcgggacggc gctggcatgg tggcaaggtc acttcgatcc gctggtcgcc ctgctggcac
     7201 taattaccgc cgggctatta cagatccttt ctaacctcgc caatgattac ggcgatgcgg
     7261 taaaaggcag cgataaacct gaccgcattg ggccgctacg cggcatgcaa aaaggggtca
     7321 ttacccagca agagatgaaa cgggcgctca ttattaccgt cgtgctcatc tgtctctccg
     7381 ggctggcact ggttgcagtg gcatgccata cgctggccga ttttgtcggt ttcctgattc
     7441 ttggcgggtt gtcgatcatt gccgctatca cctacaccgt gggcaatcgt ccttatggtt
     7501 atatcggtct gggtgatatt tccgtactgg ttttctttgg ctggttgagc gtcatgggga
     7561 gctggtattt acaggctcat acattgattc cggcactgat ccttccggcg accgcctgtg
     7621 gcctgctggc aacggcagta ctgaacatta ataacctgcg tgatatcaat agcgaccgcg
     7681 aaaatggcaa aaacacgctg gtggtgcgct taggtgaagt gaacgcgcgt cgttatcatg
     7741 cctgcctgct gatgggctct ctggtatgtc tggcgctgtt taatctcttt tcgctgcata
     7801 gcctgtgggg ctggctgttc ctgctggcgg caccattact ggtgaagcaa gcccgttatg
     7861 tgatgcggga aatggacccg gtggcgatgc gaccaatgct ggaacgtact gtcaagggag
     7921 cgttactgac taacctgctg tttgttttag ggatattcct aagccagtgg gcagcataac
     7981 taacaaatat caattaacaa ttgatgattt tgccaacagc ccacatagcg cgatatactg
     8041 aaaattctcg cagcaactga atgttaagcc tatgaaatac gatacttccg agctttgtga
     8101 catctatcaa gaagatgtta acgtcgtgga accgctgttc tccaactttg gcggacgggc
     8161 gtcgtttggc ggacaaataa tcacggtaaa atgtttcgag gacaacgggt tgctgtacga
     8221 tctgctcgaa cagaatggcc gtggtcgtgt tcttgtcgtc gatggcggtg gttctgttcg
     8281 tcgcgcactg gtcgatgctg aactggcgcg tctggcagta caaaatgaat gggaaggtct
     8341 ggttatttac ggcgcggtgc gtcaggtaga tgacctggaa gagttggata tcggcatcca
     8401 ggcgatggcg gcaattccgg ttggtgccgc tggcgaaggc attggcgaaa gcgatgtccg
     8461 cgtcaatttt ggcggtgtca ccttcttctc cggcgaccat ctttatgccg acaataccgg
     8521 gattattctt tcagaagatc cgctggatat tgaatgataa gaaaggcacc gcaaggtgcc
     8581 ttttttctgc gttacctgtt ggcctacaca gtaaagaaat tacgcgaaag atgaagcgta
     8641 atcagacctc ttccatgcga cccagcaggg cctgcagacg ttcctgccag ccgttctgct
     8701 gttctttcag atggttgttc tcacgctcca gctcttcgcg ctgatgctgg gcattttgaa
     8761 cttcctgcga cagtgagttg tttttttctt tcagctcttc gatttccatc tgcaacagag
     8821 tgatggtatc aatcgcctgc tgtacttttg cttccagttt ctcaaacact tctaatgaca
     8881 ttgtcatacc tctcctgaat tgcaaggcgt tgatggataa aaaatcctcg tcccgattac
     8941 cggtgacgcc ttaataaata cgagcgcact ttagttagct ccgattgtat gaagccgcgc
     9001 catcgctgtc cagcggcacg ccttgcagat tacggtttgc cacacttttc atccttctcc
     9061 tggagacata atccacacca atcgaaaatg ttaataaatt tgttgcgcga atgatctaac
     9121 aaacatgcat catgtacaat cagatggaat aaatggcgcg ataacgctca ttttatgacg
     9181 aagcacacac attttaagtt cgatatttct cgtttttgct cgttaacgat aagtttacag
     9241 catgcctaca agcatcgtgg aggtccgtga ctttcacgca tacaacaaac attaactctt
     9301 caggatccga ttatgagtca aacatcaacc ttgaaaggcc agtgcattgc tgaattcctc
     9361 ggtaccgggt tgttgatttt tttcggtgtg ggttgcgttg cagcactaaa agtcgctggt
     9421 gcgtcttttg gtcagtggga aatcagtgtc atttggggac tgggggtggc aatggctatc
     9481 tacctgaccg caggggtttc cggcgcgcat cttaatcccg ctgttaccat tgcattgtgg
     9541 ctgtttgcct gtttcgacaa gcgcaaagtt attcctttta tcgtttcaca agttgccggc
     9601 gctttctgcg ctgcggcttt agtttacggg ctttactaca atttattttt cgacttcgag
     9661 cagactcatc acattgttcg cggcagcgtt gaaagtgttg atctggctgg cactttctct
     9721 acttacccta atcctcatat caattttgtg caggctttcg cagttgagat ggtgattacc
     9781 gctattctga tggggctgat cctggcgtta acggacgatg gcaacggtgt accacgcggc
     9841 cctttggctc ccttgctgat tggtctactg attgcggtca ttggcgcatc tatgggccca
     9901 ttgacgggtt ttgccatgaa cccagcgcgt gactttggtc cgaaagtctt tgcctggctg
     9961 gcgggctggg gcaatgtcgc ctttaccggc ggcagagaca ttccttactt cctggtgccg
    10021 cttttcggcc ctatcgttgg cgcgattgta ggtgcatttg cctaccgcaa actgattggt
    10081 cgccatttgc cttgcgatat ctgtgttgtg gaagaaaagg aaaccacaac tccttcagaa
    10141 caaaaagctt cgctgtaatg tgactacggg acaactaaac atgactgaaa aaaaatatat
    10201 cgtcgcgctc gaccagggca ccaccagctc ccgcgcggtc gtgatggatc acgatgccaa
    10261 tatcattagc gtgtcgcagc gcgaatttga gcaaatctac ccaaaaccgg gctgggtaga
    10321 acacgaccca atggaaatct gggccaccca aagctccacg ctggtagaag tgctggcgaa
    10381 agccgatatc agttccgatc aaattgcagc tatcggtatc actaaccagc gtgaaaccac
    10441 tattgtctgg gaaaaagaaa ccggcaagcc catctataac gccattgtct ggcagtgccg
    10501 tcgtaccgca gaaatctgcg agcatttaaa acgtgacggt ttagaagatt atatccgtag
    10561 caataccggt ctggtgattg acccgtactt ctctggcacc aaggtgaagt ggatcctcga
    10621 tcatgtggaa ggctctcgcg agcgtgcgcg tcgtggtgaa ttgctgtttg gtacggttga
    10681 tacgtggctt atctggaaaa tgactcaggg ccgtgtccat gtgaccgatt acaccaacgc
    10741 ctctcgtact atgttgttca acatccatgc cctggactgg gacgacaaaa tgctggaagt
    10801 gctggatatt ccgcgcgaga tgctgccaga agtgcgtcgt tcttccgaag tgtacggtca
    10861 gactaacatt ggcggcaaag gcggcacgcg tattccaatc tctgggatcg ccggtgacca
    10921 gcaggccgcg ctgtttggtc agttgtgcgt gaaagaaggg atggcgaaga acacctatgg
    10981 cactggctgc tttatgctga tgaacactgg cgagaaagcg gtgaaatcag aaaacggcct
    11041 gctgaccacc atcgcctgcg gcccgactgg cgaagtgaac tatgcgctgg aaggtgcggt
    11101 gtttatggcg ggcgcatcca ttcagtggct gcgcgatgag atgaagttga ttaacgacgc
    11161 gtacgattcc gaatactttg ccaccaaagt gcaaaacacc aacggcgtat atgtggtccc
    11221 agcatttacc gggctgggtg cgccgtactg ggacccgtat gcgcgcgggg cgattttcgg
    11281 tctgactcgt ggggtaaacg cgaaccacat tatccgcgcg acgctggagt ctattgctta
    11341 tcagacgcgt gacgtgctgg aagcaatgca ggccgactct ggtattcgtc tgcacgccct
    11401 gcgcgtggat ggcggcgcag tagcaaacaa tttcctgatg cagttccagt ccgatattct
    11461 cggtacgcgc gttgagcgcc cggaagtgcg cgaagtcacc gcattgggtg cggcctatct
    11521 tgctggtctg gcggttggct tctggcagaa cctcgacgag ctgcaagaga aagcggtgat
    11581 tgagcgcgag ttccgtccag gcatcgaaac cactgagcgt aattaccgtt acgcaggctg
    11641 gaaaaaagcg gtcaaacgcg cgatggcgtg ggaagaacac gacgagtaat gtatgccgga
    11701 tgaagcgttt ttgccgcatc cggtagtccc gaaacgtgcg ggggcaaccc cgcacacatc
    11761 aataatccct cccttcccct gtgctacact tcgcgccatt ccttactgct tagagtttgc
    11821 tatgagacga gaacttgcca tcgaattttc ccgcgtcacc gaatcagcgg cgctggctgg
    11881 ctacaaatgg ttaggacgcg gcgataaaaa caccgcggac ggcgcggcgg taaacgccat
    11941 gcgtattatg ctcaaccagg tcaacattga cggcaccatc gtcattggtg aaggtgaaat
    12001 cgacgaagca ccgatgctct acattggtga aaaagtcggt actggtcgcg gcgacgcggt
    12061 agatattgct gttgatccga ttgaaggcac gcgcatgacg gcgatgggcc aggctaacgc
    12121 gctggcggtg ctggcagtag gcgataaagg ctgcttcctc aatgcgccgg atatgtatat
    12181 ggagaagctg attgtcgggc cgggagccaa aggcaccatt gatctgaacc tgccgctggc
    12241 ggataacctg cgcaatgtag cggcggcgct cggcaaaccg ttgagcgaac tgacggtaac
    12301 gattctggct aaaccacgcc acgatgccgt tatcgctgaa atgcagcaac tcggcgtacg
    12361 cgtatttgct attccggacg gcgacgttgc ggcctcaatt ctcacctgta tgccagacag
    12421 cgaagttgac gtgctgtacg gtattggtgg cgcgccggaa ggcgtagttt ctgcggcggt
    12481 gatccgcgca ttagatggcg acatgaacgg tcgtctgctg gcgcgtcatg acgtcaaagg
    12541 cgacaacgaa gagaatcgtc gcattggcga gcaggagctg gcacgctgca aagcgatggg
    12601 catcgaagcc ggtaaagtat tgcgcctggg cgatatggcg cgcagcgata acgtcatctt
    12661 ctctgccacc ggtattacca aaggcgatct gctggaaggc attagccgca aaggcaatat
    12721 cgcgactacc gaaacgctgc tgatccgcgg caagtcacgc accattcgcc gcattcagtc
    12781 catccactat ctggatcgca aagacccgga aatgcaggtg cacatcctct gattgatttg
    12841 atcgattgag ccttccagtc cttcgggact ggaatttttt tgttcggaga acgaagataa
    12901 ggcaagttaa tcaaaacagg agaaaaacat ggctgattgg gtaacaggca aagtcactaa
    12961 agtgcagaac tggaccgacg ccctgtttag tctcaccgtt cacgcccccg tgcttccgtt
    13021 taccgccggg caattcacca agcttggcct tgaaatcgac ggcgaacgcg tccagcgcgc
    13081 ctactcctat gttaactcgc ccgataatcc cgatctggag ttttacctgg tcaccgtccc
    13141 cgatggcaaa ttaagcccac gattggcggc actgaaacca ggcgatgaag tgcaggtggt
    13201 tagcgaagca gctggcttct ttgttctgga tgaagtaccg gactgcgaaa cgctatggat
    13261 gctggcaacc ggtacagcga ttggccctta tttatcgatt ctgcaactag gcaaagattt
    13321 agatcgcttc aaaaatctgg tcctggttca cgccgcacgt tatgccgccg acttaagcta
    13381 tttgccactg atgcaagaac tggaaaaacg ctacgaaggg aaactgcgca ttcagacggt
    13441 ggtcagtcgg gaaacggcag cggggtcgct caccgggcgg ataccggcgt taattgaaag
    13501 tggggaactg gaaagcgcga ttggcctgcc gatgaataaa gaaaccagcc atgtgatgct
    13561 gtgcggcaat ccacagatgg tgcgcgatac acaacagttg ctgaaagaga cccggcagat
    13621 gacgaaacat ttacgtcgcc gaccgggcca tatgacagcg gagcattact ggtaagcggt
    13681 tacttatcga taaacggcac gatgagcaaa tccgcactca tcttattgat catcccgcga
    13741 tatgccggca tcaaacggtt gataaatgag tgatgatgac cacagacaag gaggtcgcac
    13801 tgctcttttt gcataatttc cagcagtgtt tccggcattt ctccgcgttc aatacgcagt
    13861 tttgtcttcg gccattgaat atttttcgtc agtttataca gcttgttatc cgacttattc
    13921 ttcaacaatt gaagaatatc ttctgttgca gggaagtaga tacccggata caactcgctt
    13981 aagccatcat caatatgaat taacgtcagg tgagcgtcat tatgtctggc gagctccaga
    14041 gctttattca ccagtaaggc atcttcttca ttcccggaaa ttgccacgcc aatgtgttta
    14101 taagccatgt ttaactcctt ctaaagcaac tccatcaagc taacgaacgc agtgataact
    14161 caaaaataat catctcggcc tggcatgaga aagtgaaggc cgcatcaagc tcaacttgcc
    14221 cctccagatc cttaatcttg taggccacgc tggccccttc atctgcagag gatgcagcgg
    14281 cggcaagctc tttcagtttg gtcataaaag actctgcttc ttcgcgggtg gcaaaaaagc
    14341 gcgaaaactt actggtacag ttgtcgttat caatcaccgt accgatatca atcgcacatc
    14401 ctttagtact gcatttatct acgacatctt tcatagggca cctctgtata ttcgcctctc
    14461 tgtcgattac gacttaagaa taatgcgccg ttaagggaag aacaaacaag taatgatctt
    14521 atggattacc attttttttg atattgctta aggtgaagag ttcagaagga ttttccgggt
    14581 attgtcattt gcatcgtgga aataacaatc caggaaaa
//
LOCUS       DAFNBJ010000122        14445 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000122 DAFNBJ010000000
VERSION     DAFNBJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.122"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            441..2072
                     /gene="oppA"
                     /locus_tag="L2X49_003528"
     CDS             441..2072
                     /gene="oppA"
                     /locus_tag="L2X49_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBN4798555.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2158..3078
                     /gene="oppB"
                     /locus_tag="L2X49_003529"
     CDS             2158..3078
                     /gene="oppB"
                     /locus_tag="L2X49_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBN4798556.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3093..4001
                     /gene="oppC"
                     /locus_tag="L2X49_003530"
     CDS             3093..4001
                     /gene="oppC"
                     /locus_tag="L2X49_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBN4798557.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4013..5026
                     /gene="oppD"
                     /locus_tag="L2X49_003531"
     CDS             4013..5026
                     /gene="oppD"
                     /locus_tag="L2X49_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBN4798558.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5023..6027
                     /gene="oppF"
                     /locus_tag="L2X49_003532"
     CDS             5023..6027
                     /gene="oppF"
                     /locus_tag="L2X49_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBN4798559.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6080..6409)
                     /locus_tag="L2X49_003533"
     CDS             complement(6080..6409)
                     /locus_tag="L2X49_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBN4798560.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6444..7904)
                     /gene="cls"
                     /locus_tag="L2X49_003534"
     CDS             complement(6444..7904)
                     /gene="cls"
                     /locus_tag="L2X49_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBN4798561.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8047..8220
                     /locus_tag="L2X49_003535"
     CDS             8047..8220
                     /locus_tag="L2X49_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBN4798562.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8275..9528)
                     /gene="kch"
                     /locus_tag="L2X49_003536"
     CDS             complement(8275..9528)
                     /gene="kch"
                     /locus_tag="L2X49_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBN4798563.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9828..10124)
                     /locus_tag="L2X49_003537"
     CDS             complement(9828..10124)
                     /locus_tag="L2X49_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBN4798564.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10348..11067
                     /gene="tonB"
                     /locus_tag="L2X49_003538"
     CDS             10348..11067
                     /gene="tonB"
                     /locus_tag="L2X49_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBN4798565.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPAVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11107..11505)
                     /gene="yciA"
                     /locus_tag="L2X49_003539"
     CDS             complement(11107..11505)
                     /gene="yciA"
                     /locus_tag="L2X49_003539"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBN4798566.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11610..12149)
                     /locus_tag="L2X49_003540"
     CDS             complement(11610..12149)
                     /locus_tag="L2X49_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBN4798567.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12179..12922)
                     /locus_tag="L2X49_003541"
     CDS             complement(12179..12922)
                     /locus_tag="L2X49_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBN4798568.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(12950..13039)
                     /locus_tag="L2X49_003542"
                     /pseudo
     CDS             complement(12950..13039)
                     /locus_tag="L2X49_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13279..13917
                     /gene="ompW"
                     /locus_tag="L2X49_003543"
     CDS             13279..13917
                     /gene="ompW"
                     /locus_tag="L2X49_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBN4798569.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(13977..>14445)
                     /locus_tag="L2X49_003544"
     CDS             complement(13977..>14445)
                     /locus_tag="L2X49_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBN4798570.1"
                     /translation="AHAMEKQAESMLESMASRIDNYPELRARIEQHLSETKNQIVQLE
                     TILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYVFEQFEIACYTSLL
                     AAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSETDGVEAKK"
BASE COUNT         3876 a         3385 c         3530 g         3654 t
ORIGIN      
        1 tgtaatcaga aaagatagac atgcttagcc aatctctatt tgattgaatt gaaagatgtt
       61 tgttaaggca tggatgcaag ctatagattc tgatacggtc aataaaagag aattgcttaa
      121 caattttgca aaatgtattg gcgagtaaga accgcatttg gtactttccg ggcaaccgcc
      181 agacgattct ttattggtaa tgagaataat taacaattaa agagcgtcgc gaaagaataa
      241 tgtgtctcga caggggagac acagtacgaa tcgacataag gtgatcgtct gaatcaccag
      301 aataaataaa gtcggtgata gtaatacgta acgataaagt aacctgacag cagaaagtct
      361 ccgagcctgt gcagggtccc aatccgggat tacacatgct ggttaatacc agtaattata
      421 atgagggagt ccaaaaaaca atgaccaaca tcaccaagag aagtttagta gcagctggcg
      481 ttctggctgc gctaatggca gggaatgtcg cgctggcagc tgatgtaccc gcaggcgtca
      541 cactggcgga aaaacaaaca ctggtacgta acaatggttc agaagttcag tcattagatc
      601 cgcacaaaat tgaaggtgtt ccggagtcta atatcagccg agacctgttt gaaggcttac
      661 tggtcagcga tcttgacggt catccagcac ctggcgtcgc tgaatcctgg gataataaag
      721 acgcgaaagt ctggaccttc catttgcgta aagatgcgaa atggtctgat ggcacgccag
      781 tcacagcaca agactttgtg tatagctggc aacgttctgt tgatccgaac actgcttctc
      841 cgtatgccag ttatctgcaa tatgggcata tcgccggtat tgatgaaatt cttgaaggga
      901 aaaaaccgat taccgatctc ggcgtgaaag ctattgatga tcacacatta gaagtcacct
      961 taagtgaacc cgttccgtac ttctataaat tacttgttca cccatcaact tcaccggtgc
     1021 caaaagccgc tatcgagaaa ttcggcgaaa aatggaccca gcctggtaat atcgtcacca
     1081 acggtgccta taccttaaaa gattgggtcg taaacgaacg aatcgttctt gaacgcagcc
     1141 cgacctactg gaacaacgcg aaaaccgtta ttaaccaggt aacctatttg cctattgctt
     1201 ctgaagttac cgatgtcaac cgctaccgta gtggtgaaat cgacatgact tataacaaca
     1261 tgccgatcga attgttccag aagctgaaaa aagagatccc ggacgaagtt cacgttgatc
     1321 catacctgtg cacttactat tacgaaatta acaaccagaa accgccattc aacgatgtgc
     1381 gtgtgcgtac tgcactgaaa ctaggtatgg accgcgatat cattgttaat aaagtgaaag
     1441 cgcagggcga catgcccgcc tatggttaca ctccaccgta tactgatggc gcaaaattga
     1501 ctcagccgga atggtttggc tggagccagg aaaaacgtaa cgaagaagcg aaaaaactgc
     1561 tggctgaagc gggttatacc gcagacaaac cgttgaccat caacctgttg tataacacct
     1621 ccgatctgca taaaaagctg gcgattgctg cctcttcatt gtggaagaaa aacattggtg
     1681 taaacgtcaa actggttaac caggagtgga aaacgttcct cgacacccgt caccagggta
     1741 cttttgatgt ggcccgtgca ggctggtgtg ctgactacaa cgaaccaact tccttcctga
     1801 acaccatgct ttcgaacagc tcgatgaata ccgcgcatta taagagcccg gcctttgaca
     1861 gcattatggc ggaaacgctt aaagtgactg acgaggcgca gcgcacagct ctgtacacta
     1921 aagcagaaca acagctggat aaggattcgg ccattgttcc tgtttattac tacgtgaatg
     1981 cgcgtctggt gaaaccgtgg gttggtggct ataccggcaa agatccgctg gataatacct
     2041 atacccggaa tatgtacatt gtgaagcact aatggcaata cgtggggcag gagtgtcctg
     2101 ctccacggtg tctgattttt atcgcattac agaaggcaca ggccagaagg tagggcaatg
     2161 ttaaaattta ttctacgtcg ctgtctggaa gcgattccga cgctatttat tcttattact
     2221 atttcgttct ttatgatgcg cctcgcgccg ggaagccctt ttaccggcga acgtacttta
     2281 ccgccagaag tgatggccaa tatcgaagcg aaatatcatc ttaatgatcc gatcatgaca
     2341 cagtatttca gctacctgaa acaactggcg cacggtgatt tcggtccatc gtttaaatat
     2401 aaagattatt cggtcaatga cttggttgca tccagttttc ccgtttctgc caaactggga
     2461 gccgcagcat ttttccttgc ggtaatactg ggtgttagtg ctggcgttat tgccgcatta
     2521 aaacaaaaca ccaaatggga ctataccgtg atggggctgg caatgaccgg ggttgttatc
     2581 cccagttttg tggttgcacc attattagtc atgatatttg cgatcatttt gcattggctg
     2641 ccgggcggtg gctggaatgg tggggcgctt aaattcatga tattgccgat ggtggcgttg
     2701 tcactcgctt atatcgccag tattgcgcgt attacccgtg gctctatgat tgaagtatta
     2761 cactccaatt ttattcgtac tgcccgggcg aaagggttac ctatgcggcg gatcatttta
     2821 cgtcacgcat taaaacctgc tctgttaccc gtgctctctt atatgggccc cgcatttgtc
     2881 ggcattatta ccggttctat ggttatcgaa accatttatg gtttgccggg gattggacaa
     2941 ctgttcgtta atggcgcatt gaaccgtgac tattccttag tgttaagcct gaccatcctg
     3001 gttggtgctt taaccatttt gtttaatgcc attgtcgatg tgctatatgc ggttatcgac
     3061 ccgaaaatcc gttactgata ctggagctcg cgatgatgtt aagtaagaaa aacagcgaga
     3121 cgctggaaaa tttcagtgaa aagctggagg tcgaagggcg cagcttgtgg caggacgcac
     3181 gtcgacgttt tatgcataac cgtgcggcgg ttgccagtct gatagtgctg gtgctgatcg
     3241 cgttatttgt aatcctggca ccgatgcttt cccagtttgc ctatgacgat actgactggg
     3301 cgatgatgtc cagcgccccg gatatggagt ccggtcacta ctttggtact gactcatccg
     3361 gtcgcgacct gcttgtgcgc gttgcgattg gcgggcgtat ctcactcatg gtcggtgttg
     3421 ctgcggcact ggtggctgtg gtcgtcggga cactttatgg ttcgctttcc ggttatctgg
     3481 gcggtaaagt ggattcggta atgatgcgtc tgctggaaat cctcaactcc ttcccattca
     3541 tgttcttcgt cattttgctg gtgacctttt tcggtcaaaa catcctgctg attttcgtgg
     3601 cgataggcat ggtttcctgg ctggatatgg ctcgtattgt gcgtgggcaa accctgagtc
     3661 tgaagcgcaa agagtttatt gaggcggcac aagttggcgg tgtatcgaca tcgggcattg
     3721 ttattcgcca cattgtgccg aatgtactcg gtgtggtggt ggtctacgca tcacttctgg
     3781 tgcccagcat gatcctcttt gaatctttcc ttagcttcct ggggttgggt acgcaagagc
     3841 cgttaagcag ctggggtgca ttgctgagtg atggcgcgaa ctcgatggaa gtctctccat
     3901 ggttattgtt gttcccagcg ggattcctcg tggtgacgct gttttgtttc aactttatcg
     3961 gcgatggctt gcgtgatgcc ctcgacccga aagatcgtta aggagtgcag ccatgagcgt
     4021 aattgaaact gcaactgtgc cgctcgcaca acaacaggct gacgcactgc tgaacgtgaa
     4081 agatttgcgt gtcaccttta gtaccccgga cggcgacgtc acggcggtca atgatttgaa
     4141 tttttcccta cgtgccggag aaacgctggg tattgtaggt gagtctggtt cgggtaaatc
     4201 gcaaactgca tttgcgttga tgggcctgtt ggctgccaac ggacgtattg gcggatcggc
     4261 aaccttcaat gggcgtgaaa tcctcaattt gccagagcat gaactcaata aactgcgcgc
     4321 tgaacaaatc tcaatgattt ttcaggaccc aatgacttcg ttgaatccct atatgcgcgt
     4381 cggtgagcag ttaatggaag tgctgatgct gcataagaac atgagcaaag ctgaggcgtt
     4441 tgaagagtcg gtgcggatgc tcgatgcggt aaaaatgccg gaagcgcgta aacgcatgaa
     4501 aatgtacccg cacgaatttt ctggcggcat gcgtcagcga gtcatgattg cgatggcatt
     4561 gctatgtcga cctaagctac tgattgcgga tgaacccact actgcgctgg acgtcaccgt
     4621 acaggcgcag atcatgacgc tattaaatga actgaagcgg gaatttaata ccgccatcat
     4681 tatgatcacc cacgatcttg gcgtggtggc ggggatctgt gacaaagtgc tggtaatgta
     4741 cgccgggcgc acgatggaat atggcaatgc gcgcgatgtc ttttatcaac ccgttcatcc
     4801 ttattctatc ggtttgctca acgcggtgcc gcgtcttgat gcggaaggtg aaacaatgtt
     4861 gaccatcccg ggtaatccgc caaacttgct gcgattaccg aaaggttgcc cgttccagcc
     4921 acgttgtcca catgcgatgg aaatttgtag tagcgcaccg ccgctggaag agtttacgcc
     4981 tggccgtctg cgtgcttgct ttaaaccggt ggaggaactg ttatgaatgc tgtaactgaa
     5041 ggaagaaaag tcctccttga aatcgccgat cttaaagtgc actttgaaat caaagatggc
     5101 aaacagtggt tctggcaacc gccgaaaacg ctcaaagccg tcgatggtgt cactcttcgc
     5161 ctgtatgaag gggaaacatt aggtgtggta ggggaatcgg gatgcggtaa gtccaccttt
     5221 gctcgcgcca tcatcggttt ggtcaaggcg accgacggtc atgttgcctg gttaggtaaa
     5281 gagttgctgg gcatgaagcc cgatgaatgg cgtgccgttc gcagtgatat tcagatgatt
     5341 ttccaggatc cgttggcatc gctaaacccg cgtatgacca tcggcgagat catcgctgaa
     5401 ccactgcgta cttatcatcc gaaaatgtca cgccaggaag ttcgcgagcg cgtgaaggcg
     5461 atgatgctga aagtcgggtt attgcctaac ctgattaacc gctatccgca tgagttctcc
     5521 ggtgggcagt gccagcgtat cgggattgct cgtgctctta ttcttgaacc gaagctgatt
     5581 atctgcgatg agccggtttc ggcgctggac gtgtcaattc aggcgcaggt ggtcaacctg
     5641 ctccagcagc tgcaacgtga gatgggattg tcattaattt ttatcgctca tgacctggcc
     5701 gtggtaaaac acatttccga tcgtgtgttg gtgatgtatc tcggccatgc ggtagaactg
     5761 gggacctatg atgaggtcta ccacaatcca ctacatcctt acaccagggc attgatgtcg
     5821 gcagtcccca tacctgatcc ggatctggag aagaacaaaa ccatccagtt actggaaggg
     5881 gaattaccgt cgccgatcaa cccgccttcc ggttgtgttt tccgtacccg ttgcccgatt
     5941 gccggtccgg agtgcgccaa aacacgtcct gttctggagg ggagtttcag acacgccgtt
     6001 tcttgcctga aagtcgatcc gctttaaaca caataagggc tgacagttgt cagccctttt
     6061 tcacgctaaa agcgattatt tattcccgcc agatgatatg gcaaagtttg tgatcttttt
     6121 cgcggcataa cagaatgcgg gcgaaaacat cgttgatttc accatcttca ctgtccgcca
     6181 gaccaatcac cacttcggca aaaaagtctg ggttcaaatc gaaatcaaca tgttcctgcc
     6241 agtcttccgc cggatcgaat aactccgcgc cgccgcgctc ttcaaactga agattgaaca
     6301 gcagaacatc agctggatcg agattgtcgg cagccagttc gagaaaaata tcgtaggcct
     6361 gctcaagcgt ttcatcttca gtcaggcgat tgtttagatc catatccata gtcactacct
     6421 gtttaacctc tgttggcgac gttttacagc aacggactga agaagtaaaa cagtcgctcg
     6481 gcgacacgtt gccataatgg acgttttagc cataaacggg catcgagcag acgtgaacgc
     6541 gaaatataat cgtcctgaac ggcggcaagg tctgcaccaa aacctttatc gtcgattgcc
     6601 agggtaatct cgaaattcag ccacagactc cgcatatcaa ggttaacggt gccaaccaga
     6661 cttagttcgc catcgaccag cacgctcttg gtatgcagta acccgccttc aaactgataa
     6721 attttaaccc cagcagcaag cagttccgta aagaatgcgc gactggccca gccgaccagc
     6781 atcgagtcat tttttcgcgg aaggataata ctgacatcca ccccgcgctg cgccgccgtg
     6841 caaatcgcat gaagtaaatc atcgcttggc acaaagtagg gcgtggtcat gatcaaatat
     6901 tcacgcgccg aataagccgc agtcaataat gcctggtgaa tgagatcttc cggaaagccg
     6961 gggccagaag caattgtgtg aatggtgtga ccgctggcct gttcaaacgg cataatattg
     7021 acatctggtg gtggcggcag aatacgtttt ccggtttcaa tctcccagtc gcaggaataa
     7081 ataatcccca tcgcggtggc gatggggcct tccatacgcg ccatcagatc aatccattgc
     7141 cctacgcccg catcttgttt gaagtagcga ggatcgacca tattcatgct gccggtgtac
     7201 gcgatgtaat tatcgatcat gatcatcttg cgatgttggc gcaggtccat acggcgtaaa
     7261 aacacacgca tcagattgac ctttaaggct tcgaccactt caataccggc attacgcatt
     7321 agctcgggcc acgggctgcg gaaaaaagcc acactcccgg cggagtcgag catcaatcgg
     7381 caatgaatac cacgccgtgc agccgccatt aatgattcag ccacctggtc cgccatgccg
     7441 ccgggctgcc agatataaaa caccatctca atattatggc gcgcgagctg gatgtcgcgg
     7501 attaacgcct gcatcacatc atctgactcg gtcatcagtt gtagctgatt ccctttgacc
     7561 ccagcgatcc cctgacgacg ctcgcaaagc ttgaataatg gcgcagcgac actgctattt
     7621 tcttcggcga agatatgctt acaggcttta aggtcgttaa gccattttgc ggtggaaggc
     7681 cacatcgctc tggcgcgctc agcgcggcgt ttgcctaaat ggagctcgcc aacggcaaga
     7741 taggcaataa ttccgactaa cggcagaatg taaataatca acagccaggc catcgcggag
     7801 ggaactgcgc gtcgtttcat tagaatgcgt aaagttacgc ctgcaatgag caaccagtat
     7861 cccagaatgg ccaaccaact caccaacgta taaacggttg tcatagatta gaaatccttt
     7921 tgaaagcgca ttgttatgag tttacgcata tggattcatc tggcaaataa aaacgcggga
     7981 aaagcactgg tcagcgtcgg gggtgggggt ataatgacca ttctgttatt gcatagagta
     8041 gttaacatga agcggagtag aacggaagtg gggcgctggc gcatgcagcg tcaggctagc
     8101 cgacgtaaat cgcgttggct tgaggggcaa tcgcgccgaa atatgcgtat ccacagcatc
     8161 aggaagtgca ttctaaacaa acagcgtaac tcgttattgt ttgcgatcta caatatctaa
     8221 atgtactggg caccattgcg gtgcccaata agtttactct tccgtcttta aactctattt
     8281 ttgcgccgat tctttactgt cagctttcgt ttcttgttca tcgttatcgc tgaaaatacc
     8341 atgacctgag ttcaacaaca ttgaaacgag catatcatta ttaatctctt caccatttaa
     8401 aactcgcgcc agaatttcgc tgccaaacag ttgcggtgag agaatgatat ccggatgtac
     8461 catcttaatc ttattcaggt ttttgctatc actgacggcg agaactgttt tgacatcact
     8521 gctcatatct tttgccgaga gtacaacaaa cgcgttatct gcatcgttat cactcagcgc
     8581 cagaatggcc cggcaacgat cgattcccgc tttctttaat actgaactgt cattactgtc
     8641 accggggata acatcagcgt tatcgcctaa gcgttgctca agttgcttga tatcatcttc
     8701 aggcaagttg ctgataaccg ttacgttttg tccgcgttga ttcagttgca ggatcgtatt
     8761 gatggcgaga atcgaatgtc cgcaaacaat aaaatgatct ttacgatgca ttgtatgatt
     8821 gtttcctttt acaagtttgt tgaatccccc gcggataagc ggaccaaaaa ttgaggtcat
     8881 agatgtggca aaaacggtaa tgccggaaat aatgaccgaa atagtgaaca atcgtgctga
     8941 ttcagaaaca gggacaatat cgccatagcc gacggttgac atggtttcta tcgaaaaata
     9001 gaacgcggtc atcaaacttt ctattcgcgg attaaaacct tcgcttaaat aaagcgcacc
     9061 gtaggttgag taaaacagta atgtcgtgaa actaataaat gcaaaaattg tcccggctgc
     9121 ggcgctactg tgggagaagt ctttgcgcag tatcagcaaa aacaccagcg taaaaatgca
     9181 aaatccaata ctaaatttca gccagggata aaaatgcagg gtgtaaatta gcgctatcaa
     9241 caacaaaatg atactgattg cccatgcgag ctttgcccgg aacagtaagc caatggagtt
     9301 caggacaaga aagacgccga gcataaatag tggggcgttt gccagcaagg agagatccaa
     9361 ctctgaaaat gctttgatat ggaaaatatc aagcaaattg accgacatac catagattgt
     9421 tttaaaaata agcaatccat ttaaaaagac ggccagcgcg agaatgtcgt gccgaagggt
     9481 cacccataaa tttgttgctg tttgtttgaa tgtagcccag tgactcactg atacctctgt
     9541 aaccacaatt ttgtttaatt atcttagcgt gcacattttc tgaacataca tgcagcgcgt
     9601 gaatgtgtta aacaatagta gatgacttta tccgcagtaa tcaaaggatt tgagcgagca
     9661 actgtacctc aggaaaggtc atttttcctg aatatgctca catcatataa agaaatacag
     9721 ataaagttat tatctgcttg tggtagtgaa tgcactgacc ggctataagg aaaggccaaa
     9781 caagaacacg gttgcaaaaa ccgtgccctt aaatattgaa tctctattca gaacactttc
     9841 ttaaatggtt tcactgaaac gtgttcatag actcctgccg ctacgtacgg gtcagcatcg
     9901 gcccaggcct gagctgcttc cagcgattca aattcagcaa taacggttga gccagtaaat
     9961 cccgcagccc ctggatcgtt actgtctacc gctggcattg gaccagctgt caacaaacga
    10021 ccttcatcat gcagtaactg taaacgtgct aaatgtgccg gacgaacgga aaggcgtttt
    10081 tcgaggctat cagctttgtc ttgagcgtaa ataacataca acacgggcac aactccttgt
    10141 tcgggaaagt tgtaaagtac gttatttgaa agggcgaaga tctgcaacgg aaagatgatg
    10201 tctttgttaa gaccatgcat aaattaaggt caattacgcc aaaaatgaca ttttcactga
    10261 tcctgatcgt cttgccttat tgaatatgat tgctatttgc atttaaaatc gagacctggt
    10321 ttttctactg aaatgattat gacttcaatg acccttgatt tacctcgccg cttcccctgg
    10381 ccgacgttac tttcggtctg cattcatggt gctgttgtgg cgggtctgct ctatacctcg
    10441 gtacatcagg ttattgaact acctgcgcct gcgcagccaa tttctgtcac gatggttgcg
    10501 cctgctgatc tcgaaccgcc acaagccgtt cagccgccac cggagccggt ggtagagcca
    10561 gaaccggaac ctgagccgat ccccgaacca ccaaaagaag caccggcggt cattgaaaag
    10621 ccgaagccga aacctaagcc aaaaccgaag ccggtgaaaa aggtacagga gcagccaaaa
    10681 cgcgatgtca aacccgtaga gtcgcgtccg gcatcaccgt ttgaaaatac ggcacccgca
    10741 cgcctgacat caagtacggc aacggctgca accagcaagc cggttaccag tgtggcttca
    10801 ggaccacgcg cattaagccg taatcagccg cagtatccgg cacgagcaca ggcattgcgc
    10861 attgaagggc aggttaaagt taaatttgac gtcacgccgg atggtcgcgt ggataacgta
    10921 caaatcctct cagccaagcc tgcgaacatg tttgagcgtg aggtgaaaaa tgcgatgcgc
    10981 agatggcgtt atgagccggg taagccaggc agtgggattg tggtgaatat cctgtttaaa
    11041 attaacggca ccaccgaaat tcagtaagca gaaagtcaaa agcctccgac cggaggcttt
    11101 tgactattac tcaacaggta aggcgcgagg ttttccttca ggatcaaccg cgacatactt
    11161 aaataatgct tctgtcgctt tatagcgttg cccgattggt tcagacgcta cttttttcac
    11221 ccacacttca atattaatgc tgaccgatgt cgtccctttc tggacacagc gtgcatagca
    11281 gcacaccaca tcgccgaccg caaccggccg taagaaagtc attccttcaa cccgcacagt
    11341 cactacgcga ccgtgggcaa tttctttcgc cagaatagcg ccgccaatat ccatttgtga
    11401 cattaaccaa ccaccaaaga tgtcaccatt ggcattggta tcggcgggca tggctaaagt
    11461 acgtaaaaca agatcgccct gagggacgtt atgtgttgta gacatggtaa aaccgactta
    11521 aaaggaaaat cacaggcgcg atgctactat gattttcctg aggagaacag aggagataca
    11581 gcgccagccc cgaaggactg gcagtctggt taggatttat cttcctgcgg catgtggcgg
    11641 tagatataga taccgcttaa caatgtgaag attaaggtaa gggcggtcag gccaaagact
    11701 ttaaagttga cccaaatatt ttgcggcagc cagaatgcga tgtagatgtt tgccagaccg
    11761 caaaggataa agaaaacagc ccaagccaga tttagcttcg accataccgg ttgcggcagc
    11821 gtgagttctt tacccagcat ccgctgaatt agcggctttt tcatcaccca ttggctgact
    11881 aacagggctc ccgcaaacag agcataaatg actgtaacct tccatttaat aaactcatca
    11941 ttgtggaaga acagcgtcaa gccaccgaag acgaccacca gaacaaaagt gatcagggcc
    12001 atcttctcaa ccttacgaaa gcgaacccag ctatagataa gcacaatcgc cgtggcgacg
    12061 atcagcgccg cagtagccgc atagatgtca taaatcttgt aaaacgcgaa aaagacaacc
    12121 agcggtaaaa aatcaagaaa ctgcttcatt ttacgattcc gtaatcaaat gcttaaggtt
    12181 attggcgaat caacatatac aggcggaaca gatagatgag caatatggca gaaatcaggt
    12241 tgctcaaggt gttcgccagt acggcaccaa tttccggggt taatgcggca aaagaagagg
    12301 caaaaagcag caacagtgtt tttgccagca accagctcag tactgcgggt gccaccagac
    12361 gcatattcgc ccaagtcagc cgcatactgc tacgcatcga ggcaaaaatg cccattttgt
    12421 cctgtaccag catcaccggt gccagggcca gtaaaatggc catgataatt cccggaacga
    12481 ccaccagcat gatgccaatc tgtactaaaa gggtagtcag aaaaatcaga ataaataact
    12541 tgggcaatat cggcgcactg gcaccaatag ctcgcagcgc actgactctc tgacccgcag
    12601 acaccagctg gataattaat attacgcctc cggcgagaat ggcgttaccg attaatcctg
    12661 aaaaagtgga cgccgctgaa gcctgcagca aaatttgttg ctgttccggt gacatattct
    12721 gaaccaggtc gaacaaccca ctactgccgc taacgggcac gccgtcattg agctgcgcaa
    12781 gctgtgcatc actgggtgag aaaacatgcc ctaacaccac tgtgataaac gcacatagca
    12841 acgataccaa cagaatggtc ataaattgat tacggaagaa atttccggtg tcacggtata
    12901 cggactgcgc cgtgatagac atgcactctc cttgagtttt atgcaggtgt taattagcgg
    12961 gcaattgtac cctggttaag cctcgggtgg cagcatcagg tcttgtatgg aaaagcatat
    13021 ctttgtaaag cggaggtaat tgctggcctt caggcgtggg caaaccgcgg caagtggcga
    13081 tcgagttaat atttgcgtag agaaaatatt taaaaactga tttaaatcac attaaccagg
    13141 attctcaatg caacttctaa attaatccag atcaataaat ggtgaattat catatgtaat
    13201 gtgatctatg taggatcatt tgttactcca atgtaggtat attcgtcacg tttttataac
    13261 cataacgacg gagcggatat gaaaaagtta acagtggcgg ctttggcagt aacaactctt
    13321 ctttctggca gtgcctttgc gcatgaagca ggcgaatttt ttatgcgtgc aggttctgca
    13381 accgtacgtc caacagaagg tgctggtggt acgttaggaa gtctgggtgg attcagcgtg
    13441 accaataaca ctcaactggg cttgacgttt acttatatgg cgaccgacaa cattggtgtg
    13501 gaattactgg cagcgacgcc gttccgccat aaaatcggca cccgggcgac cggcgatatt
    13561 gcaaccgttc accatctgcc gccaacactg atggcgcagt ggtattttgg tgatgccagc
    13621 agcaaattcc gtccttacgt tggggccggt attaactaca ccaccttctt tgataatgga
    13681 tttaacgatc atggcaaaga ggcggggctt tccgatctca gtctgaaaga ttcctgggga
    13741 gctgccgggc aggtgggggt tgattatctg attaaccgtg actggttggt taacatgtca
    13801 gtgtggtaca tggatatcga taccaccgcc aattataagc taggcggtgc acagcaacac
    13861 gatagcgtac gcctcgatcc gtgggtgttt atgttctcag caggatatcg tttttaattc
    13921 cacaaaaaaa cgaccccgta atatacgggg tcaataagga catggcataa agtgtattat
    13981 ttcttcgctt ctacgccgtc agtttcagag cgaattaaaa atttctcagt tgtttgcgga
    14041 atatgctgaa tcagccagtc ggccatttgc ttttcctcat ttaaaatcgc ttcgatggtt
    14101 ggaattgaag ctgtatcacc ggcatttttt gctgctgcta atagtgaggt gtaacaggcg
    14161 atttcaaatt gctcgaagac atatccgcta atagagcctt tgactatttc atcagaaggg
    14221 aatataccac cgattgactg cccaagcgca gccattttac tcatggaatc tttaatgact
    14281 gaacgtgaaa tgtcattacg atcaagaata gtttccagtt gaacaatctg gtttttggtt
    14341 tcactaagat gttgttcaat acgagcgcgt agttcaggat aattatctat acggctggcc
    14401 atggattcaa gcatagattc ggcttgcttt tccattgcgt gggcg
//
LOCUS       DAFNBJ010000123        14224 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000123 DAFNBJ010000000
VERSION     DAFNBJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.123"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..197)
                     /locus_tag="L2X49_003545"
     CDS             complement(<1..197)
                     /locus_tag="L2X49_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBN4798571.1"
                     /translation="MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDDLASGGRD
                     DRPGLTACLKSLRDGDVLVVW"
     gene            323..3208
                     /locus_tag="L2X49_003546"
     CDS             323..3208
                     /locus_tag="L2X49_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBN4798572.1"
                     /translation="MPRRVTLTDRQKDALLRLPTSQTDLLKHYTLSDEDLGHIRLRRR
                     VHNRFGFALQLCVLHYPGRVLAPGELIPAEVIEFIGAQLGLGADDLVDYAAREETRHE
                     HLAELRGLYGFRTFSGRGASELKEWLFREAEMAVSNEDIARRFVAECRRTRTVLPATS
                     TIERLCAAALVDAERRIETRIASRLPMSIREQLLALLEETADDRVTRFVWLRQFEPGS
                     NSSSANRLLDRLEYLQRIDLPEDLLAGVPAHRVTRLRRQGERYYADGMRDLPEDRRLA
                     ILAVCVSEWQAMLADAVVETHDRIVGRLYRASERICHAKVADEAGVVRDTLKSFAEIG
                     GALVDAQDDGQPLGDVIASGSGWDGLKTLVAMATRLTATMADDPLNHVLDGYHRFRRY
                     APRMLRLLDLRAAPVALPLLEAVTALRTGLNDAAMTSFLRPSSKWHRHLRAQRAGDAR
                     LWEIAVLFHLRDAFRSGDVWLTRSRRYGDLKHALVPAQSIAEGGRLAVPLRPEEWLAD
                     RQARLDMRLRELGRAARAGTIPGGSIENGVLHIEKLEAAAPTGAEDLVLDLYKQIPPT
                     RITDLLLEVDAATGFTEAFTHLRTGAPCADRIGLMNVILAEGINLGLRKMADATNTHT
                     FWELIRIGRWHVEGEAYDRALAMVVEAQAALPMARFWGMGTSASSDGQFFVATEQGEA
                     MNLVNAKYGNTPGLKAYSHVSDQYAPFATQVIPATASEAPYILDGLLMNDAGRHIREQ
                     FTDTGGFTDHVFAACAILGYRFAPRIRDLPSKRLYAFNPSAAPAHLRALIGGKVNQAM
                     IERNWPDILRIAATIAAGTVAPSQILRKLASYPRQNELATALREVGRVERTLFMIDWI
                     LDAELQRRAQIGLNKGEAHHALKRAISFHRRGEIRDRSAEGQHYRIAGMNLLAAIIIF
                     WNTMKLGEVVANQKRDGKLLSPDLLAHVSPLGWEHINLTGEYRWPKP"
     gene            3392..3556
                     /locus_tag="L2X49_003547"
     CDS             3392..3556
                     /locus_tag="L2X49_003547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798573.1"
                     /translation="MSDLRLHIRALEVKYHDGCPALVDALVADFLRNLNEQYLTVYMR
                     VLAFSGCFHD"
     gene            4216..4437
                     /locus_tag="L2X49_003548"
                     /pseudo
     CDS             4216..4437
                     /locus_tag="L2X49_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612267.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4796..4933
                     /locus_tag="L2X49_003549"
                     /pseudo
     CDS             4796..4933
                     /locus_tag="L2X49_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612240.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5055..5942)
                     /locus_tag="L2X49_003550"
     CDS             complement(5055..5942)
                     /locus_tag="L2X49_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase SFO-1"
                     /protein_id="HBN4798574.1"
                     /translation="MVKNTLRQTTLMVATVMPLLFGSAPLWAQSANAKANIQQQLSEL
                     EKNSGGRLGVALIDTADNSQILYRGDERFPMCSTSKVMAVSALLKQSEMDKNLLAKRM
                     EIKQSDLVNYNPIAEKHLDTGMTLAELSAAAIQYSDNTAMNKILEHLGGPAKVTEYAR
                     TIGDKTFRLDRTEPTLNTAIPSDKRDTTSPLAMAKSLQTLTLGKALGEPQRAQLVEWM
                     KGNTTGGASIRAGLPATWVVGDKTGSGDYGTTNDIAVIWPANHAPLVLVTYFTQPQQN
                     AEARKDVLAAAAKIVTEGL"
     gene            6075..6950
                     /locus_tag="L2X49_003551"
     CDS             6075..6950
                     /locus_tag="L2X49_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4798575.1"
                     /translation="MRSNPPLNALRAFEASARHLSFTRAALELYVTQAAVSQQVRLLE
                     ERLGMVLFKRLPRGLEMTEESRALFAVLTDAFGHIEKAFRQFEGGQFQEVLTVAAVGT
                     FAVGWLLPRLDRFRQEHPFVKLRLRTNNNVVNLAAEGLDFAIRFGTGLWPSTHNEMLF
                     NAPLTVLCTPATAKRLVTPADLLQEDLLRSYRAEEWENWFAAAGLQAVRVNGAIFDSS
                     RLMIESAIHSGGVALAPAKMFVRELTTAQLVRPFATEINMGSYWLTHLKSKAMTPAME
                     IFSEWLLKEAAEDNT"
     gene            7653..8003
                     /locus_tag="L2X49_003552"
     CDS             7653..8003
                     /locus_tag="L2X49_003552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798576.1"
                     /translation="MHRLYTYLCAIHALPSSVKTVNKTETLKRFSEGENTIEECEILY
                     VFNNDVQILYRMESETFQSNDVCHECWVSYDVVHDGGYAISPQKKQFYNRCQENFWLK
                     MQAQLDGKYKTPAS"
     gene            complement(8324..8461)
                     /locus_tag="L2X49_003553"
     CDS             complement(8324..8461)
                     /locus_tag="L2X49_003553"
                     /inference="COORDINATES: protein motif:HMM:NF016847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBN4798577.1"
                     /translation="MLGRYMSHIPAKNFKKVRYYGFLSNRKRCELLPKVYEALQMEAR
                     K"
     gene            complement(8656..9825)
                     /locus_tag="L2X49_003554"
                     /pseudo
     CDS             complement(8656..9825)
                     /locus_tag="L2X49_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400937.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            9830..10324
                     /locus_tag="L2X49_003555"
                     /pseudo
     CDS             9830..10324
                     /locus_tag="L2X49_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694157.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            10337..10832
                     /locus_tag="L2X49_003556"
                     /pseudo
     CDS             10337..10832
                     /locus_tag="L2X49_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11182..11952
                     /locus_tag="L2X49_003557"
     CDS             11182..11952
                     /locus_tag="L2X49_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBN4798578.1"
                     /translation="MNRSVPDVLPTIRNASQLSPAALDMAKLLALLAMIIDHTNTVFL
                     SPAWPVMYALGRMAFPLFTLIWAMNVQRTPERLQKRASRLWIWAVITQPVFSLAFWHH
                     QPWWALNILFVFAGVTQLIALQYQHGLKGMFAGYLLLALMIWPLQPASYGLAGITLAI
                     SMATVSGSDTPGVQRLAAITAVLSLICLNGLSHLLDMPAETLLLATLPTLAFPLAVVS
                     FCRQICPEGRRRFMPRNFFYYAYAGHLGIIGLVQSAFG"
     gene            12084..12410
                     /locus_tag="L2X49_003558"
     CDS             12084..12410
                     /locus_tag="L2X49_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798579.1"
                     /translation="MNHESDLTNHGFSEEDIVRLRASSAKTGTEMDTLLNDLANRFKA
                     LIWVLSVMLLIFVATLLAGSRMHAISFGVAILVLGTLFTITMPLKLAYKSWHYRRNLI
                     SQRHQK"
     gene            complement(12385..13191)
                     /locus_tag="L2X49_003559"
     CDS             complement(12385..13191)
                     /locus_tag="L2X49_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HBN4798580.1"
                     /translation="MDIILPGNKSQARVWAETMINLEARKLVNTANIVGARHLGDGLT
                     RLKFIDEIKSVINGEFERARRAKSDEECMTCLRNLQAENTSLLEQSRQLQTGYAKLYA
                     QIKYVRDEDRMVGYVISGIKVVLAGMQGVFGFVLTSSMTPVGVLAGAILIVDSANTIT
                     REVGRQFLNEPDTQGMFGDGAMNIAKFMGFEPSTGLGIYNGIALAGNIYSVYGLMARP
                     GTWRLFNYISTDFYRKIDTMSRGKLTMKIVGWGVKAKVVFDLITSDDAGK"
     gene            complement(14071..>14224)
                     /locus_tag="L2X49_003560"
     CDS             complement(14071..>14224)
                     /locus_tag="L2X49_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBN4798581.1"
                     /translation="RRPHERLHAMSEWIYRTLPPDEAYWCTSERLKALAIQHLYQLDL
                     ALSPPD"
BASE COUNT         3336 a         3679 c         3782 g         3427 t
ORIGIN      
        1 ttccagacca ccagcacatc gccgtcacgc aatgacttga ggcaggcagt caagccaggg
       61 cgatcatcac gaccgccgga agcaagatca tcatagatat tgtcccgttc gacacctgcg
      121 gcgcgcaagg cgtcgtgctg caggtcgaga gactgcgagc catcggcttt ggagacgcgg
      181 gcatatccga tcagcatgta tcacaaacgt tggtttgagg cggcgcttcg gccacgattg
      241 cattgacctc tggaaatgta tctcaaccag cttcataaat aaagcgtctt gaacgctatc
      301 agattttgaa aaaggaacat gtatgccgcg tcgcgtcact ctaaccgatc ggcagaaaga
      361 cgcgctgttg cgcttgccga cttcacagac ggatttgctc aagcactata cgctgagtga
      421 tgaagacctt gggcatatca ggctgcgtcg gcgcgttcac aacaggttcg gcttcgccct
      481 gcaattgtgt gtcctgcact atcccggccg ggtgctggct ccaggcgaac tgatccctgc
      541 agaggtcatc gaatttatcg gagcgcagct tggcctgggt gccgacgatc tcgtagacta
      601 tgctgcccgc gaggaaacac ggcacgagca tcttgccgag ttacgggggc tctacggctt
      661 ccgcaccttc tccgggcgtg gtgcgagcga gctgaaggaa tggttgttcc gagaagcgga
      721 gatggcggtg tcgaacgagg atatcgcccg tcgcttcgta gccgagtgcc gacgcacccg
      781 cactgtcctt cccgcgacat ccacgatcga gcggctttgt gccgcggctc tcgtcgatgc
      841 cgagcgacgc atcgagacga ggatcgccag tcggctgcct atgtcgatcc gagaacagtt
      901 gctggcattg ctcgaggaga cggctgatga tcgggtgacc cgttttgtgt ggctgcgcca
      961 gttcgagcct ggctcgaact cttcgtcggc caaccggctg ctcgaccggc tcgaatatct
     1021 gcaacgcatc gatctccccg aggatctgct tgccggcgtt cctgcccatc gggtgactcg
     1081 tctgcgcagg cagggtgaac ggtattatgc cgacggcatg cgcgatctcc cggaggacag
     1141 gcggcttgcg atcttggctg tttgcgtctc ggaatggcag gcgatgttgg ccgacgcagt
     1201 ggtcgaaacc cacgaccgga tcgtcggccg tctctaccgt gcttcggagc gtatttgcca
     1261 tgcaaaggtc gcagacgaag cgggggtggt gcgtgacacc ctgaaatcct tcgccgagat
     1321 cgggggcgcc ctggtcgatg cacaggatga tggccagccg ctgggcgatg tcatcgcgag
     1381 tgggtcaggg tgggacggct taaaaaccct tgttgcaatg gcaaccaggc tgaccgccac
     1441 catggccgac gatccgctca atcatgtgct cgacggttat caccgcttcc gccgatacgc
     1501 tccacgcatg ttgcgcctgc tcgatctgcg agctgcgccc gttgcactgc cgcttctgga
     1561 agcggtgacg gcccttcgta ccggtttgaa cgatgccgcg atgaccagct tcttgcggcc
     1621 cagctcgaaa tggcatcgcc accttcgggc ccagagggct ggcgacgctc gcctatggga
     1681 gatcgcggtg ctgttccatc tgcgcgatgc gttccgctcc ggagatgtct ggcttactag
     1741 gtcccggcgc tatggcgatc tgaaacacgc actcgttccg gcacaatcca tcgcggaagg
     1801 cggtcgtctc gctgtgccat tgcggccgga ggaatggctg gcagaccggc aagctcgcct
     1861 cgacatgcgg ttgcgcgagc ttggccgtgc cgctcgcgca ggcacgatcc cgggcgggtc
     1921 gattgaaaac ggcgttctgc atatcgagaa actcgaagcc gccgcgccga caggcgccga
     1981 agatctggtg ctcgatctct acaagcagat cccgcccacg cgcatcaccg atctcctgct
     2041 ggaggtggat gcggcgaccg gcttcaccga agcgttcacc catctgcgca caggagcacc
     2101 ctgcgctgac cggatcgggc taatgaacgt tatcttggcg gaagggatca acctcggctt
     2161 gcgcaaaatg gcggatgcga caaacaccca caccttctgg gaattgatcc gcattggacg
     2221 gtggcatgtc gagggcgaag cctatgaccg ggcgctggcc atggtggtcg aggcacaggc
     2281 agcgttaccc atggcccggt tctggggcat gggcacgtcg gcttcgagcg acggacagtt
     2341 cttcgtcgct acagagcaag gtgaggccat gaacctggtc aacgcgaaat atggcaatac
     2401 cccgggcctg aaagcctata gccacgtctc cgaccaatat gcgccgttcg caacccaggt
     2461 gattcctgca acggcaagcg aagcgcctta catcctcgat ggcctgctga tgaacgatgc
     2521 tggacgccat atccgcgagc agttcaccga cacgggcggc ttcaccgatc acgtctttgc
     2581 cgcatgtgcc attctcggct accggttcgc tccgcgcatc cgcgacctgc catccaaacg
     2641 gctctacgcg ttcaatccgt cggccgcccc ggcgcacctg cgagcgttga tcggcggaaa
     2701 ggtcaaccaa gccatgatcg agcgcaattg gcccgacatc ctgcgcatcg ccgccaccat
     2761 tgctgccggg accgtcgcgc caagccagat tctgcggaaa ctcgcctcct atccgcggca
     2821 gaacgagctc gcgacagccc tgcgggaagt cggtcgcgtc gagcgcaccc tgttcatgat
     2881 cgactggatt ctggatgccg aactccaacg gcgtgcccag atcgggctca acaaaggcga
     2941 agctcatcat gcgctgaagc gggcaatcag cttccaccgc cgcggtgaaa tccgcgaccg
     3001 ttccgccgaa ggccagcatt accgcatcgc cggcatgaat ctgctcgccg ccatcatcat
     3061 cttctggaac accatgaagc tcggcgaggt cgttgcaaac cagaaacgcg atggaaagct
     3121 gctatcgccc gatctcttgg cccatgtttc gccgctcgga tgggaacaca tcaatctcac
     3181 cggagaatat cgctggccaa agccttagcg taggattccg ccccctcccg caaacgaccc
     3241 cttatagggc gaggcgtaca ggctctgggt atatcggtaa cgaactgctg gagcagtacc
     3301 cggccgctaa cgacgatctg ccgtaggctt cgtaataaat tatcagggac atatacaact
     3361 gcgtgacgat ctggctgcgc tatccagcac catgagcgac ttgcgcctgc atattcgagc
     3421 tctggaagtt aagtatcacg acggatgtcc cgcgctggtt gatgcgctgg tcgctgattt
     3481 cctgcgcaat ctcaacgagc agtatctgac ggtttacatg cgcgtactgg cgttcagcgg
     3541 ctgttttcac gactgactgc gatcggacgg tttacggcac gctaacgcgg agatacgccc
     3601 cgattaaggc ttactctgga ccactccgac gactcacata accttcttcg tggcttaaat
     3661 tgggattgtt caagcttggc agggtgagag attattacgg cttacgccgt aatgcttttt
     3721 tcatcctcac ttcgcggaat aaacacgcac ataatatgtg gaaatatcag ggtaaacagg
     3781 ctgactagga gcggtacgag aaagttctgt attaaaaaac taagtatccg cgaaacagcc
     3841 gatgcgacag gttacagtcc ctctctgata tgtacgattt cagttactgc aaaggaaaac
     3901 tctgtcagca gtggagtagt taagttacaa ctatattaca aaagggatat gatttttcag
     3961 gctttttcaa agtgatccag cttcatctcc atgatccacc attcggctac ttacatgagt
     4021 aattccgggt catcattctt gttggcaaaa aaagcataaa atgacacgcc aacatcatcc
     4081 ccttttgccg ctgtcttagc ttcacatacc ttcacaatct tcgctcaaat gctggctctc
     4141 attatctttc tcttatagtt gctgaggaca catgctctca gacgctgtcg aatgggcaga
     4201 attggttaaa gatcctcacg cccgggaacc ccaacggaca atgcgactgt ggagtcgttc
     4261 aatggcaggc tacggcagga gtgcctgaat gaaaactggt tcatgtcgct ggaagatgcc
     4321 cggtccaaaa tcgaggcttg gcgcatacac tataaccaga agcgtcccca ttctactctg
     4381 ggatggatga ccccgtccga atttgcagaa aaatctgtcg gttgccagaa tatgtaacca
     4441 acatgaagcc ggttattcct gattatgact ggagcatatt cgggggcggg gtcagaagcc
     4501 ggatattcct aattatgaat ggatcacata ctgggagcgc gtcaatatga agacattact
     4561 atcatcggtg tgtatgaatc aacggggaga aggtcaaagg aaaccggagc gacagaaata
     4621 gcaaagatga taagtatcgc acgttccacg gtcaataaaa tcatcgaaga gtcgaaaatg
     4681 tgaatgtcgg cagtcggttt ttttaaaagg tcaatataat gaaaataaaa gatttttacc
     4741 gaccacgtta gcattatcgt ggaaatgtgc gcgaaaccct taatgcgaca aagcctatca
     4801 gaacgcattg gcggaaaagg tcaacgagat actgaagacg aagtttttgc tgtcacgccc
     4861 ggcagatctg tagcaggcaa gaaagatggt gagggaatca atagcaattt acaacagagt
     4921 tcagcctcac taggcactaa aatacaaaac gcccgatacg gtgcatcagg cgttttacag
     4981 gcataaaagt gtcaacctat gctaggacta gtcagtcgaa atcccggcgc agatacgcgg
     5041 tgcatatccc tgattcaaag cccttcggtg acaattttag cggcagcagc caacacgtct
     5101 ttgcgggctt ctgcattctg ctgtggctga gtgaaatagg ttaccaacac caacggtgcg
     5161 tggttcgctg gccaaatcac ggcgatatcg ttagtggtac cgtaatcacc actgccggtt
     5221 ttatcaccga ccacccacgt ggctggcaga cctgcgcgaa tgctggctcc gccggtagtg
     5281 ttccccttca tccattcaac cagttgagca cgctgtggtt cacccagcgc cttgcccaaa
     5341 gttagggttt gcaggctttt tgccatcgcc agcggcgagg tagtgtcacg cttatcgctg
     5401 ggaatggcag tatttaaagt aggctcggta cgatcgagac gaaaggtttt atcaccaata
     5461 gtacgcgcat actctgtcac ttttgccggg ccgccaagat gctcaaggat cttgttcatc
     5521 gccgtgttgt cactgtactg gatggcggcg gcactgagct ctgcaagggt catcccggta
     5581 tccaggtgtt tttcggcgat cgggttgtag ttgaccagat cggattgttt gatttccatc
     5641 cgcttagcca aaagattttt atccatctcg ctctgtttta acaacgccga caccgccatc
     5701 accttgctag tgctacacat gggaaaacgt tcatccccac gatacaggat ctgcgaatta
     5761 tcggcggtat cgatcagcgc cacgccaagg cggccaccgg agtttttctc gagctcggac
     5821 agttgctgct gaatattcgc tttggcatta gcggattgag cccataatgg tgcgctaccg
     5881 aacagcagcg gcataaccgt agcgaccatc agggtggttt gacgtaatgt atttttaacc
     5941 atgttttgcc tcacataaaa tggattaact cagcgggttg ttctgcagat ggcgagaact
     6001 atcttctttt agaccgttat tgacaagcga caagaatttt ctctaacata aagaaaatct
     6061 tttggataat acctatgcgt tctaaccctc ccctgaatgc tctacgtgct tttgaagcct
     6121 ctgccagaca tttgagtttc actcgggcag cgctggagct ctatgttacc caggctgccg
     6181 ttagccaaca ggttcggctg cttgaagagc gcctgggcat ggtgctgttt aaacgattac
     6241 cccgtggttt agaaatgaca gaggaatcac gggcgctgtt tgccgtgtta accgacgcct
     6301 ttggccatat tgaaaaagcc ttccggcagt ttgaaggggg gcagttccag gaggtgctta
     6361 ccgtggctgc cgtcggcact tttgccgtgg gctggctgtt accacgtctc gatcggttca
     6421 ggcaagagca tccttttgtc aaactgcggc tgcgcaccaa caacaacgtt gttaatctgg
     6481 ccgccgaagg gcttgatttc gccatccgtt ttggcacggg tttgtggcca tcaacccata
     6541 acgagatgct gtttaatgct ccgctgaccg tattgtgcac gccagctacg gctaagcggc
     6601 tcgttacccc agccgatctg ctgcaagagg atttactgcg ttcttaccgt gctgaagagt
     6661 gggaaaactg gtttgctgcc gctggcttgc aggctgtgag agtgaatggc gccatctttg
     6721 actcatcccg tctgatgatc gagagcgcta tccattccgg cggtgtggca ttagccccag
     6781 cgaaaatgtt tgtccgggag ctaaccaccg cgcagctggt gcgcccattt gctaccgaaa
     6841 tcaatatggg cagttactgg ttaacccatt taaaatcgaa agcaatgacc ccggcgatgg
     6901 aaatatttag cgaatggtta ctgaaagaag cggcagaaga taatacgtag gggcgcagca
     6961 tgctgtgccc ctcgggtttc cagacttatt gcttgtggtg agtcaggcgg gtagcaaaaa
     7021 aatggttttt cggaagaaat gcgggcttcc gaaaacaaac agagacagga catgtccgtt
     7081 caaacattca ggaacaggta acgggattta acggaacctg aatgcccccc gataactgtc
     7141 atcatcttgg ctgaagacac aggtattagg ggagccaaaa gcaggcgcgt tgcctgtgat
     7201 ggattatgtt gtaactagag tcctgagttg ctgtaggata tatcactgaa ggtattgaat
     7261 aacacgcccg taagcagccc ttacggctcg ctaacgcgga gatacacccc gactgcggct
     7321 tcgccttgtc atgagcactc cgacggctca caaaagcttc tgtatcgctt ttagcaggtt
     7381 tatgtctggc agggtacagg gatggattgg ctgaacctat caaacctgta acaggctgcg
     7441 ccgggttagg gtgccaatca ccaataaagc tatattcagc gttggctggc acctccatgc
     7501 cgctaagcgg cattcaaata actggatgcg cactgctcgg cagtttatat tagggactcc
     7561 tgagtgctgc cctttcgtct tttcaccaac aagcatttgc tttattcttg ggaaagtatt
     7621 tccgacagtg acaagtttgg aggcagattg ccatgcatcg actttacaca tacctttgtg
     7681 ctatccatgc actgccgtca tcggtaaaaa cggtcaataa aacggaaacc ctcaagcgat
     7741 tctctgaagg ggaaaacacg atagaagagt gcgaaatcct ttacgtgttt aataatgacg
     7801 ttcagatttt atatagaatg gaatccgaaa ccttccaatc gaatgacgtt tgtcatgagt
     7861 gttgggtcag ttatgatgtg gtacatgatg gcggatacgc catttcaccg cagaaaaagc
     7921 agttttataa tcgttgccaa gaaaatttct ggctgaaaat gcaggctcaa cttgatggga
     7981 aatataaaac accagcctct tgatggttag gaaaatgaat catcgttatt aaaaatatcc
     8041 ttaccatgaa tggtatcgtt gggtgctaat gcaatttgtg ccgataaaat gtggttttcg
     8101 gtgcatttct agcctgaaag acgtttttca gactcactta tcccgcagcc tgtgacatgt
     8161 gacagaagcc atcgtttccg gtcaatgctg tgcatctgcc actaactccg tggcgtgtca
     8221 gccagcctat gcaccgctga agcgtagccg attgccgtac agaaggcatt tattcggatc
     8281 cgtgtgcagg aaccctttac gcggcgaagc ccggctgctc cgtttatttc cgcgcctcca
     8341 tctgcagagc ctcatacact tttggcagca gttcgcaccg tttccggttg gacaagaagc
     8401 catagtagcg gaccttctta aaattctttg ccgggatatg actcatatag cgcccgagca
     8461 tctcttcctg tgtctgcgtc tgctgccggt aatgctgaga tacagtccgt cagcagtcct
     8521 aacctcgacg tactcacccc aatctcgcac atgaacacag aggtgctttt agcgtgtacg
     8581 cctttgcagc ttccgtatta agttccctgg tatcactgat ataggtgttc ttagccggaa
     8641 ccccagattt ttccgttacc ccagtttcag ccccgcaacc agtgcatcta ggtcaataat
     8701 ttcattgtta ctcttgaagg tgtaatgccc gttcaataaa atatactgcc aggctaccgg
     8761 tgatatccgg gtcagtgcct caataccctt gttgttgcct tctgcttcca gcagttccag
     8821 taatcgtgaa aggatctcgg agttgtaata aacgctctcg tagcagatca actgcccgca
     8881 ctgattgcag atttccgttt aaaggtcatt tttcccggtt agctccttct ttctaccgac
     8941 tttggcgact gcagcgcgca gctagtgata cgattcaatc cggttctggg agcgacgtat
     9001 atcgcgttcc agctgcggat ctcgcgaata cttcagcgtg taatgctgcg aacaagccgg
     9061 tcatattcaa atactgcctg tcttgtcggg ttagttgttg tgtgagtaca cagtttgcag
     9121 aacagcgttc cctgcgtcat ttccttcagt cctagcgtgg cgacaatacg ctcgagattg
     9181 cttttttccc cgggtgataa ggtccaggtc aatttgcccg acgggctgaa tcagacatcg
     9241 agggttctct ggtgcagtac agcacctgca gctgcctgtt caggtcggtg aaatgaggtt
     9301 caaagcacaa gccaaaccag tgcaggatcg caaagttggc tttgttgatg ctgtgcatat
     9361 caccggtgat ggctgtgggt ttcacctctg aggtattgcg ataccagata tcaaaaacat
     9421 ggtgaccttc atagtcattt gtaccaatca gatagccata gatcgggatg tggttacaca
     9481 gcagcatgta ggctaccata cctttaccgc agccgaaata tttacgcgaa tacctggctt
     9541 tactgtctgt cgttcaacgc caaatttttg cccgtcaacg gcaccataaa gggtttccgg
     9601 gtcaaacgaa tagagcggga aaatcggcag ttccttaacg gtatctgtaa tgcattcttt
     9661 ggctgtgaga agcgacgcca ggcgaaggta ctgttcgtac attgtttcca gaacatggaa
     9721 cggaatatcg ctggttcgtg ccatcacgtg atgaccatga ttcatcgcct gagcaacgat
     9781 gaccgctatc aggctgtctg catcagcgta tttttttgca taacgaataa tgaaacggaa
     9841 gttttgctcg ctggtcctgt tcgttgtgtc gttttcggcc agtgcagata tcagtggccg
     9901 gattgtgcgc gtcctggacg gtgataccgt tgagatgctt gagccgggaa aacaactgac
     9961 gctcattcgc ctggctggca tcgatgcgcc ggaaaagagt cagccctttg gctagcgttc
    10021 ccgtcaggaa ctctcatcga tggtggctca gcggcatgta agcgtcaccg gaagtgataa
    10081 ggatcgctat ggacgtttgc tcggcacctt gtggcttggc gtgacggctg tgaatgccga
    10141 acagatacgc aaaggcctgg cctggacata ccgctaccac ggaaaacccg cccggccaga
    10201 ctacgctgtt ctggaggctg aagcccgccg tcagtcagtc ggactctggt cagaaccagg
    10261 tcagacagag ccctggcgct ggcgtagtct gcatcaggat gggctaaaaa agaataatga
    10321 ttaaggagat ttctgaatga cgttaaaatt aacagaaagc aagctggaaa accttgttga
    10381 tactttaaat gccattatat gtggtgaagt aaatattacg cgtgagcagc gtgaaaatat
    10441 ggtcaggacg gttgcaattc tgggaggctt aacggaacga cagcgcctca tcactgcgga
    10501 aaaagaagct aaaacgcagg taaaagaaga aaagaaaaaa accacgcctt ccggatgaag
    10561 tgtttccgcg ttcaggaaaa ccgtgggcac cggaggacct ggaccttatt cattcgatta
    10621 ttgctgatat ttcggatgac agaatcgatg atcatatact ctggctgtcg aagaaactgc
    10681 agcgcacccc ttatgccatc accctgaaaa ttgtcagtga aggccgtatg aacgaggaat
    10741 gggctaaagg gtggaagcct gccgcaaaat ctatttgtgc ggattttgct ctggtcaggg
    10801 caaagacttc agcaaacaat acagataatt aatgaacgtt cgtcactcat atgaaatgag
    10861 ttgataaatt aactaaaatt tataacgtta atatgaatat tttatcaaga attttcctgc
    10921 tcaactaaat ataatattca tgcagccgat aataagagtg taagaaaggg ggcggtgtga
    10981 acgattttca tattttaagt atttgtattc agaataagga tgtggcaggc gcaatgcgcg
    11041 tgctgtgtga taaatcagaa tttgcggtgc gcaagattct ggagaaacta aaggtcaggg
    11101 tcacaacgca aaccggcagg gcattctggc attatgttca gggctggctg ttaaccgcgt
    11161 gtcgccaggg gaatctacag catgaatcgt tcagtacccg acgttcttcc cacaatccgg
    11221 aatgccagcc agctcagtcc cgctgctctc gatatggcca agctcctcgc acttctggcc
    11281 atgattatcg accacaccaa tacggtgttt ctgtcgccag cctggccggt gatgtatgcg
    11341 cttggccgga tggccttccc cctgttcacg cttatatggg cgatgaacgt acagcgcacc
    11401 ccggaacgac tgcagaagag agccagtcgc ctgtggatct gggcagtcat cactcagccg
    11461 gtgttcagcc tggccttctg gcatcaccag ccctggtggg ccctgaacat tctgttcgtg
    11521 tttgccggcg tcacgcagct gattgccctg cagtaccagc acggcctgaa aggaatgttt
    11581 gcgggatatc tgctcctggc cctcatgatt tggcccctgc agccggcaag ctacgggctg
    11641 gcaggaataa cgctggccat cagtatggca acggtttctg gcagcgatac acccggagtg
    11701 cagcggctgg cagccatcac tgccgtgctg tcactgattt gtttaaacgg gttatcgcat
    11761 ctcctggata tgcccgcaga aacattatta ctggccacgc tgcctactct ggcattcccc
    11821 ctagcagtgg tttcattctg caggcaaatc tgtccggagg gccgcaggcg tttcatgccg
    11881 cgtaatttct tttattatgc ttacgccggc cacctgggga ttattggtct ggtacagtct
    11941 gcttttggct gacttaggat gacgtgatgg ctatcagtga gaaagagctg atagcccgta
    12001 accacctgag aggttatcgg tcgggcttaa ctggttatta gcgcagggca tgttaaactt
    12061 cccgcaggga aaaggagaga agcatgaacc atgaaagtga tctgacgaac cacggcttca
    12121 gtgaagagga cattgtccgg ctgcgggcca gttcagctaa aacgggtacg gagatggata
    12181 ccctactgaa cgatctggca aaccgtttta aggccctgat atgggtgctg tcagtcatgt
    12241 tactgatttt tgtggccaca cttttggctg gctcgaggat gcatgccatt tcctttggcg
    12301 tggctattct tgttcttggc actctgttta cgattaccat gcctttgaag ctggcctata
    12361 aatcatggca ttaccgccgg aatctcattt cccagcgtca tcagaagtga tcagatcgaa
    12421 aacaacctta gcctttaccc cccatcccac aattttcatt gtcagtttgc cccgggacat
    12481 ggtgtcgatt ttccggtaaa aatcggtgct tatatagtta aacagacgcc atgttccagg
    12541 tctggccatt aatccataga cactgtagat attgcctgca agcgcgatcc cgttgtatat
    12601 ccccaaccct gttgacggtt caaatcccat gaatttagcg atattcatgg caccatcacc
    12661 aaacataccc tgcgtgtcag gttcattcag gaactgacga ccaacttcac gggttatcgt
    12721 gttggccgag tcgacaatca gaatagcccc ggcaagaacg ccaacggggg tcattgagga
    12781 agtcagcacg aagccaaata cgccctgcat acctgccagc accactttaa ttcctgagat
    12841 gacataaccg accatccggt cttcatccct gacgtatttg atctgggcat atagcttcgc
    12901 ataaccggtc tggagctgac ggctctgctc aagcagactg gtgttttcgg cctgcaggtt
    12961 acgcaggcag gtcatgcact cttcgtcaga ctttgcccgc cgggcacgct caaattcgcc
    13021 attaatgaca gatttaattt catcgatgaa tttgagacgc gtcaatccat cccccagatg
    13081 tcgggcgcca acaatattag cggtattgac gagtttgcgg gcctccagat taatcatggt
    13141 ttctgcccag actcgtgcct ggcttttgtt tcccggaaga attatatcca tttttctcat
    13201 cctgattgaa aattacccat taaagcttat cgcgctcccg gatttaggca accctgcttt
    13261 aaagggtaaa tgtatgccgc gaagcggcat ggagacgcca gccggagctg aaccgggtga
    13321 ttctggtgag gggcgtcgta accggcatca gccgttacgg attgataggt tctgccaatc
    13381 ccatcccttt ccctgcaaag ctatacccgc ttaaagcttt ccgattgcta atgtgcgcgg
    13441 tcggagtgag cccgtcgagg ccagaggccg ctgacgggct gtatctccgc gttagcgccg
    13501 tgaggcgctt acgagcgtgt acagcgggct tgccgcgacg ctaactgcag ctgctttgat
    13561 ttctagttac aacatccctc ttaaagcgag ggtgttccgg ccctttgggc cggggcggcg
    13621 tcggtttagc aggggtcagg tatgtatgcg gtgcctggtt ctggcgagaa cggggcccgg
    13681 cgcttgcgac gggaggtact tttgatttcc ttctgagcgc gcggccgtga ggccgctgca
    13741 cgcgaaggca ttttacgtta tccacagagt ggccgtagac aaagatctac tctggaatgt
    13801 aatgaacaga gagataaaaa aggtgaagta agactgtttt gcggaatgtc ttttattcat
    13861 ttccgggtta ctccacttat tcgtttgcgg atttctgtgt aaaaaaaaga gactgtggcc
    13921 attgcgggcc ataacgaccg gcagaacgtc atggtgaccg gaagacgaaa ggagtgaaag
    13981 atgaaaaatg agcataatgg ccgaaaaaat ccagcacgaa aagccccggt gaggggcctg
    14041 taaacaggaa gactgctgtg tcgtctgtga ctagtccggt ggagaaagcg ccagatccag
    14101 ctggtaaaga tgctggatag ccagagcctt caggcgctca gatgtgcacc agtacgcttc
    14161 gtcaggcggc agggtgcggt aaatccattc agacattgca tggagtcgtt catggggtcg
    14221 tctc
//
LOCUS       DAFNBJ010000124        14117 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000124 DAFNBJ010000000
VERSION     DAFNBJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.124"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            163..1221
                     /gene="citC"
                     /locus_tag="L2X49_003561"
     CDS             163..1221
                     /gene="citC"
                     /locus_tag="L2X49_003561"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBN4798582.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1236..1532
                     /gene="citD"
                     /locus_tag="L2X49_003562"
     CDS             1236..1532
                     /gene="citD"
                     /locus_tag="L2X49_003562"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBN4798583.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1529..2437
                     /gene="citE"
                     /locus_tag="L2X49_003563"
     CDS             1529..2437
                     /gene="citE"
                     /locus_tag="L2X49_003563"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBN4798584.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2448..3980
                     /gene="citF"
                     /locus_tag="L2X49_003564"
     CDS             2448..3980
                     /gene="citF"
                     /locus_tag="L2X49_003564"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBN4798585.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3984..4535
                     /gene="citX"
                     /locus_tag="L2X49_003565"
     CDS             3984..4535
                     /gene="citX"
                     /locus_tag="L2X49_003565"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBN4798586.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4510..5388
                     /gene="citG"
                     /locus_tag="L2X49_003566"
     CDS             4510..5388
                     /gene="citG"
                     /locus_tag="L2X49_003566"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBN4798587.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5439..6902
                     /gene="citT"
                     /locus_tag="L2X49_003567"
     CDS             5439..6902
                     /gene="citT"
                     /locus_tag="L2X49_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBN4798588.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPNGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7016..7822
                     /gene="rna"
                     /locus_tag="L2X49_003568"
     CDS             7016..7822
                     /gene="rna"
                     /locus_tag="L2X49_003568"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBN4798589.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8052..8462
                     /gene="rnk"
                     /locus_tag="L2X49_003569"
     CDS             8052..8462
                     /gene="rnk"
                     /locus_tag="L2X49_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBN4798590.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8693..9931)
                     /locus_tag="L2X49_003570"
     CDS             complement(8693..9931)
                     /locus_tag="L2X49_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HBN4798591.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10152..10580
                     /gene="uspG"
                     /locus_tag="L2X49_003571"
     CDS             10152..10580
                     /gene="uspG"
                     /locus_tag="L2X49_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBN4798592.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10701..12266)
                     /gene="ahpF"
                     /locus_tag="L2X49_003572"
     CDS             complement(10701..12266)
                     /gene="ahpF"
                     /locus_tag="L2X49_003572"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBN4798593.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12437..13000)
                     /gene="ahpC"
                     /locus_tag="L2X49_003573"
     CDS             complement(12437..13000)
                     /gene="ahpC"
                     /locus_tag="L2X49_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBN4798594.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13371..14117
                     /gene="dsbG"
                     /locus_tag="L2X49_003574"
     CDS             13371..14117
                     /gene="dsbG"
                     /locus_tag="L2X49_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBN4798595.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASDGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3344 a         3738 c         3566 g         3469 t
ORIGIN      
        1 tcacgcagaa atactcctta ggatgtatag cgaaaggaga aaaagatata cctcgatcac
       61 ccccctttct cccaagtgaa aataaaaggt tatcagtttg caacattgaa caacattcgt
      121 tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg caatgatatt
      181 ttcacccgcg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca attcctgcat
      241 gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac ccgcgatgaa
      301 aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc gatcagtgaa
      361 tccgttcgcg gtgaaggact ggcgctgaca ttagccactg aactgataaa cctcgcctat
      421 gagcggcaca gcacgcatct gtttatttat accaaaaccg aatacgaggc gctgttccgc
      481 cagtgcggtt tttccacgct gaccagcgta cccggcgtga tggtgctgat ggaaaacagc
      541 gccacgcgac tgaaacgcta tgccgaatcg ctgaaaaaat ttcgtcatcc agggaacaag
      601 attggctgca ttgtgatgaa cgccaatccc ttcacgaatg gtcaccgtta tctgattcaa
      661 caagctgcgg cacagtgcga ctggttgcat ctgtttttag tcaaagaaga ttcttcacgc
      721 ttcccctatg aagaccggtt ggatctggtg ttaaaaggca ccgccgatat tccacgcctg
      781 actgtgcatc gcggctccga atacatcatc tcccgcgcta cgttcccttg ctacttcatt
      841 aaagaacaga gcgtcattaa ccattgttac accgaaattg atctgaagat tttccgtcag
      901 tacctcgctc ccgcgctggg tgtaactcac cgctttgtcg gtactgaacc cttttgtcgc
      961 gttaccgccc agtacaacca ggatatgcgc tactggctgg aaacgccgac tatctccgca
     1021 ccgcccatcg aactggttga aattgagcgg ctgcgttacc aggagatgcc gatatccgct
     1081 tcccgggtac gtcaactgct ggcgaaaaac gatctcacgg ctatcgcgcc gctggtccct
     1141 gcagtcacgc tgcattattt gcagaacctg cttgagcact cccgccagga cgcggcagct
     1201 cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag cccgccgttg
     1261 caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat acgcaggata
     1321 tcgacctgca aatcaatagc agcgttgaga aacagtttgg cgatgcaatt cgcaccacca
     1381 ttctggacgt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc gatgataaag
     1441 gcgcactgga ctgcatttta cgtgcacgac tggaagccct gctggcacgc gccagcggta
     1501 tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac aacgtaaaac
     1561 tcgcacccgc cgcagcatgt tgtttgtgcc tggtgccaat gccgcgatgg tcagcaactc
     1621 cttcatctac ccggctgatg ccctgatgtt tgacctcgaa gactccgtag cattgcgtga
     1681 aaaagacacc gcccgccgca tggtttacca cgcgctgcaa catccgctgt atcgcgatat
     1741 tgaaaccatt gtgcgtgtca acgcgctgga ttccgaatgg ggtgtcaacg acctggaagc
     1801 cgtcgttcgc ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg ctcaggatgt
     1861 tctggatatc gaaaaagaga tcctgcgtat cgaaaaagcc tgtggtcgtg aacccggcag
     1921 caccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcag tggaaatcgc
     1981 tcacgcttcc gagcgtttga tcggtatcgc cctcggtgca gaagactatg tgcgcaacct
     2041 gcgtacagaa cgctccccgg aaggaactga actgctgttc gcacgctgtt ccattttgca
     2101 ggccgcgcgc tctgcgggta ttcaggcgtt cgataccgtc tattccgacg ctaacaacga
     2161 agccggattc ctgcaagaag ccgcccacat caaacagctg ggctttgacg gcaaatcgct
     2221 gatcaacccg cgtcagattg atctgctgca caacctctac gcaccgaccc agaaagaagt
     2281 ggatcacgcc cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag gcctcggcgt
     2341 ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgcgccc gtctggtgct
     2401 ctcccgtgca gaactttccg gcatccgcga agaataaggc aatcaaaatg acgcagaaaa
     2461 ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcgct gaatgcgatc
     2521 ttgccgcttt ccagaactcg ccaaagcaaa cctaccaggc tgaaaaagcg cgcgatcgca
     2581 aactgtgcgc caacctggaa gaggcgattc gtcgctctgg tttacaggac ggcatgacgg
     2641 tttccttcca tcacgctttc cgtggcggtg acctgaccgt caatatggtg atggacgtca
     2701 tcgcgaagat gggctttaaa aacctgaccc tggcgtccag ctccctgagt gattgccatg
     2761 cgccgctggt agaacacatt cgccagggcg tggttacccg catttatacc tctggcctac
     2821 gcggtccact ggcggaagaa atctcccgcg gtctgctggc ggaaccggtg cagatccact
     2881 ctcacggcgg tcgtgtgcat ctggtacaga gcggcgaact gaatatcgac gtggctttcc
     2941 tcggcgtccc gtcctgtgat gaattcggta atgccaacgg ctacaccggt aaagcctgct
     3001 gcggctccct cggctatgca atggttgatg ccgacaacgc aaaacaggtt gtgatgctta
     3061 ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat caggttgatt
     3121 taatcgtcaa agttgatcgc gttggcgatg ctgcaaaaat cggcgctggt gctacccgta
     3181 tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg attgtcaact
     3241 caggctactt caaagaaggt ttctccatgc aaaccggcac cggcggcgca tcgctggcgg
     3301 taacccgttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac ttcgccctcg
     3361 gtggtattac cgcgacgatg gttgatctgc acgaaaaagg tctgatccgc aaactgctgg
     3421 atgtgcagag ctttgacagc catgctgcgc aatcgctggc ccgtaacccc aatcacatcg
     3481 aaatcagcgc caaccagtac gctaactggg gttcgaaagg cgcatcggtt gatcgtctcg
     3541 acgtggtggt actgagcgcg ctggaaattg acacccagtt caacgttaac gtgctgaccg
     3601 gctctgacgg cgtactgcgt ggtgcttccg gtggtcactg cgataccgcg attgcctctg
     3661 cgctttccat catcgtcgcg ccgctggtac gcggtcgtat tccgactctg gtggataacg
     3721 tgctgacctg catcaccccg ggctccagtg tcgatattct ggtcacagac cacggtatcg
     3781 cagttaaccc ggcacgtccg gaactggcag aacgtctgca ggaagcgggc attaaagtgg
     3841 tttccattga gtggctgcgc gaacgtgcgc gtctgctgac cggtgaacca cagccgattg
     3901 aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg atcgatgttg
     3961 tgcatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc caccatgcgg
     4021 tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa cacgtctggc
     4081 tcaagcgcca tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg ccgattaaag
     4141 acagcgaggt cacacgccga atttttaatc atggcgtgac agccttgcgt gccttagccg
     4201 caaaacaggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc gggccggagg
     4261 gcatgttgag cattgccgcc ccggctcgcg acctcaagct cgccaccatt gagcttgaac
     4321 atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacgcccgaa ggcgaaattc
     4381 tctcccgccg cgactattca ctgccgcctc gccgctgcct gttgtgcgag caaagcgcag
     4441 ccgtctgcgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc atggaggcac
     4501 tgctgaacga tgtcgatgcc tgcaacgtca actaaaacca caaagcttgc gacgtcatta
     4561 atcgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa tctgtcaccg
     4621 aaaccaggcc tcgtggatcg cattaactgc ggtgcgcaca aagatatggc gctggaagat
     4681 ttccaccgca gcgcgctggc gattcagggc tggttacccc gtttcattga atttggtgcc
     4741 tgtagtgcag aaatggcacc ggaagcggta ctccacggat tacgcccaat tggtatggct
     4801 tgcgaaggtg atatgttccg cgccactgcg ggcgtaaaca cgcataaagg cagcattttt
     4861 tctttagggc tgctatgtgc ggcaattggc cgtttgcttc agctcaacca gccggtaacg
     4921 ccaacaaccg tttgttctac ggcggcaagt ttctgccgtg gcctgaccga tcgcgaactg
     4981 cgtaccaata attcacaact gacggcaggt caacggttgt accagcagct tggccttacc
     5041 ggcgcacgcg gtgaagccga agcgggttat ccactggtga tcaatcacgc cttgccgcat
     5101 tacctcactc tgctggatca ggggttagat cctgaactgg cattgctcga taccttgctc
     5161 ctactgatgg cgatcaacgg cgataccaac gttgcatcgc gcggtggcga ggggggcctg
     5221 cgctggctac agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg aacccccgcc
     5281 gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatct cagtccaggc
     5341 ggcagtgccg acctgctgat ccttacctgg tttttagcac agatttaatt atttaagcac
     5401 ttgataaatt tggaaatatt aattttcgga gaacccgtat gtctttagca aaagataata
     5461 tatggaaact attggcccca ctggtggtga tgggtgtcat gtttcttatc cctgtcccca
     5521 acggtatgcc accacaggca tggcattact tcgctgtgtt tgtggcaatg attgtcggca
     5581 tgatcctcga gccaattccg gcaacagcga tcagttttat tgcggttact atttgcgtta
     5641 ttggcagtaa ttacctgctc tttgatgcca aagaattagc tgacccagcg tttaatgcgc
     5701 aaaaacaggc gctgaaatgg ggtctggctg gtttttccag caccacggta tggctggtat
     5761 ttggcgcatt tatttttgca ttagggtatg aagtttccgg gttaggtcgt cgcattgccc
     5821 ttttcctggt gaaattcatg ggcaaacgca cgctgacgtt gggttatgcg attgtcatta
     5881 tcgacattct gctggcaccg tttacaccgt ccaacaccgc gcgtaccggg ggtacggttt
     5941 ttccggtcat taaaaacctg ccgccgctgt ttaaatcatt ccccaacgat ccgtccgcgc
     6001 gtcgtattgg cggctatttg atgtggatga tggtcattag taccagtctg agttcgtcca
     6061 tgtttgtcac cggtgcggca ccaaacgtgc tgggtctgga gttcgtcagc aaaattgccg
     6121 gtatccagat tagctggttg cagtggttcc tctgcttcct gccggttggg gttatcttgc
     6181 ttatcattgc gccgtggctt tcctacgtgc tgtacaaacc ggaaatcaca cacagtgaag
     6241 aagtggcaac ctgggcgggt gatgaactaa aaaccatggg tgcgctgaca cgcagagagt
     6301 ggacgctgat tggccttgta ttgctcagct taggtttgtg ggtatttggc agtgaagtca
     6361 ttaatgctac tgcggttggt ctgctggcag tttcgctaat gctggccctg cacgttgtgc
     6421 cttggaaaga tattacccgc tataacagcg catggaacac gctggtcaac ctggcaactc
     6481 tggttgtgat ggctaacggc ctgactcgtt ctggctttat tgactggttc gccggtacca
     6541 tgagtacgca cctggaagga ttctcaccaa acgcaacggt gattgtactg gttctggtgt
     6601 tctactttgc acactacctg tttgccagcc tgtctgcgca caccgcaacc atgctgccgg
     6661 ttattctggc cgtcggtaaa ggtattccgg gcgtaccaat ggaacaactg tgtatcctgc
     6721 tggtgctgtc tatcggtatc atgggctgtc tgacgccgta tgcaaccggt cctggggtga
     6781 ttatttacgg ctgtggctat gtgaaatcaa aagattactg gcgtcttggc gcaatcttcg
     6841 gggtgattta catctctatg ttgctgttgg ttggctggcc gattctcgcc atgtggaact
     6901 aatacgttcg ccaaacgctg aataaatcat cacgcccgcc aggtaagccc acctggcggg
     6961 cttttttatg atttaataga tagtattcag tgtatgtatg agttccacac ccattatgaa
     7021 agcattctgg cgtaacgccg cgttgctcgc ggtttctctg cttcccttct cttctgccaa
     7081 cgccttagcg ttgcaggcaa aacagtatgg cgattttgat cgctatgtcc tggccctctc
     7141 ctggcaaacc ggattttgcc agagtcaaca cgatcgaaat cgtaacgaac gagatgaatg
     7201 tcgcctgcaa accgaaacga ccaacaaagc tgattttctg accgtacatg gtctgtggcc
     7261 aggattgcct aaatcggttg ctgcccgtgg tgttgatgaa cgccgctgga tgcgcttcgg
     7321 ttgcgctact cgcccaatcc cgaatctacc agaagcgcac gccagccgga tgtgttcatc
     7381 gccggaaacc ggattatcac tggaaacggc cgctaaacta agtgaagtca tgccaggagc
     7441 tggcggacgt tcctgcctgg aacgctacga atatgccaaa cacggtgcct gctttggttt
     7501 tgatccggac gcatacttcg gtacgatggt acgcctgaat caagaaatta aagagagcga
     7561 agccggaaaa ttccttgcgg ataattacgg taaaacagtg agccgccgtg actttgacgc
     7621 cgcctttgcc aaaagctggg gaaaagagaa cgtgaaagca gttaagctaa cgtgccaggg
     7681 taaccctgcg tatttgactg aaattcagat ctcgatcaaa gctgacgcca tcaacgctcc
     7741 gctttctgca aactcattct tgccacaacc acacccaggc aactgtggca aaacctttgt
     7801 gattgataaa gtgggttatt aatcatctga tttacaaaac acgctaattc gtcttccata
     7861 aaccgtgacg attaagaatt atacataatg cgctgcattg aggaattccc tgaatgtgac
     7921 gcaaatcact tcaaagagtg agcaacagtc agtatcatca tgtgagttaa accctcgccg
     7981 cctgacggtg agggttttct tttgggtatg tttatctgcg acactcgcag taccgacgac
     8041 atggagtaaa aatgtccaga ccaactatca tcattaacga cctggatgcc gaacgcatcg
     8101 atattctgct ggagcaaccc gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag
     8161 agttggatcg cgcccaaatg tgttcgccag aagagatgcc acacgacgtg gtgacaatga
     8221 acagccgggt taaattccgc aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt
     8281 atccggcaaa aatgaccgat agcaatactc agctttccgt tatggctccg gtaggtgccg
     8341 cactgctggg gctgcgcgtt ggcgattcca ttcactggga acttccgggc ggcgttgcaa
     8401 cccaccttga agtgctggaa ctcgaatacc agccagaagc tgctggcgac tacctgcttt
     8461 aatcccatct gcacaagcga taactcgctt tgcccagagg atgcactcgc atcctcttta
     8521 cgcaaaatca gcaatttttc ctggttctca ctgttcactt ttgctgcaaa attattccgt
     8581 ttcgtttgtg ggaataacca tggcagaagc attttacatc ctgataggat ttctgattat
     8641 ggcggcgatt atcgtcatgg ctgtccttta cctcgaaaac cactcctgac gatcatattg
     8701 ttccccccgg catcgcattc accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt
     8761 gtgcaccggg taccagaatc accttacggc actcctcttc acgtttttcg aaaatctcat
     8821 atccccgggc ggcctcttca aacggcatat agtgggtaac aatttcttct ggtttcagta
     8881 atcctttctc aattaacggt aataattctc ccagccatgc gtgaacgtgg gtctgtccca
     8941 ttttaaacgt caacccttta tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag
     9001 cgtagacgcc cggtacgcta acaatgccgc cacgcctgac cgccgcaata cactggcgca
     9061 acgctttacc gctgctgccc tccagtttca ggttagtcag caccgtttcc gtggtgctgc
     9121 ctttcgcttc aaaaccgacg gcgtctatta ctgcatccac gccccggtga cctgccgttt
     9181 gttcaataat tgactgtgcc ggatcgctat cttcatcgaa attaatcggg atcgcgccgt
     9241 agcggtcagc ggcgaaacgc aagcggtagg gatgatgatc aacaacaaaa atctgttccg
     9301 caccgagcaa ccgtgcacag gcgattgtca acaatcccac tggaccggca ccatagactg
     9361 caacgcttga accttgttgg atctgcgcat tttttgctgc ctgccatgcc gttggcagaa
     9421 tatcagaaag gaaaagggct ttatcatctg aaagcaaagg cggtacttta aacggcccca
     9481 cattcccttt agggacgcgg acatattccg cctgtccacc aggaacgcca ccatacaggt
     9541 gactataacc aaacaatgcc gcgggagctg gtatctgttt tttattgagc gcagcgcctt
     9601 tacccgcatt ggtattttcg caggcggcat attgctgcat tcgacagaaa aaacagtcgc
     9661 cacaagcaat gacaaacgga attaccaccc ggtcgccttt ttgcaaattt tttacgtcct
     9721 ttccggtttc aacgacttcc cccataaatt catgaccaaa aatatcgcca tgtttaacct
     9781 ggggtatttt gcctcgataa agatggaggt cagagccaca gatcgccgtt gccgtaatac
     9841 gcagaataat atcatctgcc tgttcaatgc ccggatcggg aacattttct acctgaacgt
     9901 gatgtgggcc gtgataagtc aatgctttca tgcaacctcc agagacagtt caaaaaagag
     9961 tcagtcttta agggtagcta tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga
    10021 attcctggtg ttcagtgaga atatattaat cacagaaacc gttcgtaagg aaaaagaacc
    10081 agacaaatat cagggataaa tattataatc atggcgaatg atttgttcat gattaacagg
    10141 gagaaagggt tatgtataag acaatcatta tgccagttga tgtatttgaa atggaattga
    10201 gcgacaaagc tgttcgccac gctgaattcc tcgcccagga tgacggagtt attcatctac
    10261 ttcacgtact acccgggtca gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc
    10321 gttttgaaga gcatctgcaa catgaagcag aagaacgtct gcaaacgatg gtcagccact
    10381 tcaccatcga tccttcccgc attaaacaac atgtccgttt tggtagcgtg cgggatgaag
    10441 tcaatgagtt ggcagaagaa ctgggggctg atgttgtagt tattggttct cgcaacccat
    10501 cgatttcgac ccatctgtta ggttctaacg cctcgagcgt gatccgccat gccaatctgc
    10561 cggtgctggt tgtgcgttaa ttgccagaat aaatatcccg ccctgcccgg agccatcccg
    10621 gcagggcttt ttaatgccat aaaaaagccg ccaggtttga ccccggcggc taagcaattg
    10681 caggtgaatc ttacttcttc ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc
    10741 agagaggctt tggcaccttc gccagtggcg atgataatct gcttgtacgg aaccgtcgta
    10801 cagtcacccg ctgcgaacac gcctttgacg ttggtttcgc atttcgcatc aatgataatc
    10861 tcacccatgc ggttacgttc gactgcgcct tccagccagt tggtgttcgg cagcagacca
    10921 atctggacga aaataccggc cagttcgatg ttgtgaatat cgccgctgac acgatctcga
    10981 tattccagac caacgacttt gctgccgtcg cctttcactt ccgtggtttg cgcattcaga
    11041 ataatgtcga cgtttttcag gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt
    11101 tctggcgcaa attcgagcag cgttacgtgc tcaacgatac ccgccaggtc gattgccgct
    11161 tccacgccgg agttaccgcc gccgataacc gctacgcgtt tgcctttaaa cagcgggccg
    11221 tcgcagtgcg ggcagtaggt cacgcctttg gtgcgatact gatcttcgcc cggaacgttc
    11281 atgttgcgcc attttgcacc ggtcgccacg ataatgctgc gtgctttcag taccgcgcca
    11341 gaagctgttt caatctggtg tagaccacct tcaaccgctg ccgggatcag tttgctggcg
    11401 ctctggctgt cgatcacatc aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc
    11461 agtttctgcc cttcagtctt cggtacagaa atgtagtttt cgatatcaac ggtatcgagg
    11521 atctgaccac caaaacgttc gcccatcaga ccggtacgga tgcctttacg tgcggagtaa
    11581 attgctgccg ctgcacccgc cgggccggaa ccgacgatta atacgtcata agcatcacgc
    11641 ttgttcagct cttctgccgc acgtttttcc gcgccagtat caattttggc aacgatttca
    11701 gtcaacgtca tgcggccctg accaaactct ttcccgttta cgaacactgc cggaacgccc
    11761 atcacgttgc gatcggtgat ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg
    11821 atgcgcgggt tcaatacgct catcaggttc agcgcctgca ccacgtccgg gcagttatgg
    11881 caagagagcg agtaataggt ttcgaattca aaatcaccgt caatatggcg aatctgctcc
    11941 agcagagact gcgcttcttt cgacggatga ccaccggtcc acagtaacgc cagtaccagc
    12001 gaggtgaact cgtggcccag cggggagcct gcaaaacgtg gcccctggtt ggaacctggg
    12061 ttggtgatca ggaaagacgg cttacgcacc ggcaagctgt tatcttcttt aaaggtgact
    12121 ttgtctgaca gttctgcgat ttcagccaac agttccttga tttctgccga tttagcgctg
    12181 tcatccagcg tggcaattaa ctcaacaggc ttggtcaatt tctcaaggta agccttgagt
    12241 tgagttttca tatttgtgtc gagcatgttt atctcctggg ctttaaacat catcatgcaa
    12301 gcggcctctg cggctacctg gatgcagctt gcatcatggt gcggggaaga aaatcgggtg
    12361 ccgcagcacc cgaagaatta ggtgaatttc cgaagtattt cgcgccggag ttggtagcgc
    12421 gaaagacgaa ggaaatttag attttaccaa ccaggtccag agacggagcc agagttgctt
    12481 caccttcttt ccatttagcc gggcaaactt cacctgggtg agaagctacg tactgtgctg
    12541 ctttgatttt acgcagcagg tcagacgcgt cacggccaat gccttcagcg gtaacttcga
    12601 ttgcctggat gataccctgc gggtcaacaa cgaaggtcgc acgatcagcc agaccttcat
    12661 cttcacgcat gttgtcgaag ttacgggtca gggcgccagt cgggtcgccg atcatcgcat
    12721 atttgatttt agcgatggtt tcagagctgc tgtgccatgc tttgtgggtg aagtgagtat
    12781 cggtagatac tgcgtatacg tctacgccca gtttctgcag ttcttcgtag tggtcagcaa
    12841 cgtcacccag ttcggtcggg catacgaaag taaagtcagc cgggtagaag aagaagacgc
    12901 tccagcggcc ttctgtatct ttttcggtga tttcgatgaa ttcgccgttt ttgaatgcct
    12961 ggtttttaaa aggtttaatt ttggtgttaa tcaaggacat ctatacttcc tccgtgtttt
    13021 cgatgagatg taaggtaacc aatttttgcc gattcggcta atgcgtttcc attatcaaat
    13081 cgataagttt taccttacaa ccttcgtaag acaactttgt gaactttgca ccgccagcgg
    13141 cgataaaaag taaaatggcc ccctttccag gtgaccgtgt aatctgaact accctttggt
    13201 atcttcagcg actgcctgca ggctgagtgt tgcccttaca aatgcaacaa cgacatggat
    13261 tacaacaccc tcataaacaa agggcaatca cctgatctaa gctcttacct atgacagtga
    13321 taggttatgc cttttactcg acttttgcac tgactgaaaa ggacaaatta atgttaaaaa
    13381 agatactttt actggctctg cttcctgcaa tcgccttcgc agaggaactt cctgctccag
    13441 taaaagcgat tgaaaaacag ggcattacaa tcatcaaaac gttcgatgcc cccggaggaa
    13501 tgaaaggtta tctcggaaag tatcaggata tgggcgtcac catctacctg actccagatg
    13561 gtaagcacgc tatctctggt tacatgtaca acgagaaagg tgaaaacctg agtaacacac
    13621 ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat gtggcaacgg atggaacaat
    13681 cccactggct cctcgacggt aaaaaagatg cgccggtcat tgtctacgtc ttcgccgatc
    13741 cgttctgccc atattgtaaa cagttctggc agcaggcgcg cccgtgggta gattctggca
    13801 aagtgcaatt aagaacattg ttggttgggg ttatcaagcc agaaagcccg gcgacagcag
    13861 cggcaattct tgcctccaaa gatcccgcaa aaacctggca acaatatgaa gcctctgatg
    13921 gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga gcaaatgaaa gtgttaagtg
    13981 acaatgagaa actgatggac gatctggggg caaatgtcac gccggctatc tattacatga
    14041 gtaaggaaaa tacgctacaa caggccgtgg ggttgcccga tcagaaaacg cttaatatca
    14101 ttatggggaa taaataa
//
LOCUS       DAFNBJ010000125        13896 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000125 DAFNBJ010000000
VERSION     DAFNBJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.125"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            85..651
                     /locus_tag="L2X49_003575"
     CDS             85..651
                     /locus_tag="L2X49_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBN4798596.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            702..3161
                     /locus_tag="L2X49_003576"
     CDS             702..3161
                     /locus_tag="L2X49_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBN4798597.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTTGINAGAWRLRSDYQLNQTDSDDNHEQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDERMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3176..3907
                     /locus_tag="L2X49_003577"
     CDS             3176..3907
                     /locus_tag="L2X49_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBN4798598.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3904..4965
                     /locus_tag="L2X49_003578"
     CDS             3904..4965
                     /locus_tag="L2X49_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4798599.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5117..6163
                     /locus_tag="L2X49_003579"
     CDS             5117..6163
                     /locus_tag="L2X49_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBN4798600.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6160..6951)
                     /gene="nei"
                     /locus_tag="L2X49_003580"
     CDS             complement(6160..6951)
                     /gene="nei"
                     /locus_tag="L2X49_003580"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBN4798601.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGVTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6987..7721)
                     /gene="pxpA"
                     /locus_tag="L2X49_003581"
     CDS             complement(6987..7721)
                     /gene="pxpA"
                     /locus_tag="L2X49_003581"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBN4798602.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMHACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7711..8643)
                     /gene="pxpC"
                     /locus_tag="L2X49_003582"
     CDS             complement(7711..8643)
                     /gene="pxpC"
                     /locus_tag="L2X49_003582"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBN4798603.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8637..9293)
                     /gene="pxpB"
                     /locus_tag="L2X49_003583"
     CDS             complement(8637..9293)
                     /gene="pxpB"
                     /locus_tag="L2X49_003583"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBN4798604.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9316..10059)
                     /gene="ybgI"
                     /locus_tag="L2X49_003584"
     CDS             complement(9316..10059)
                     /gene="ybgI"
                     /locus_tag="L2X49_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBN4798605.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10330..11811
                     /gene="dtpD"
                     /locus_tag="L2X49_003585"
     CDS             10330..11811
                     /gene="dtpD"
                     /locus_tag="L2X49_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBN4798606.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVQMIWLYQALKFRNRALALES"
     gene            complement(11853..13271)
                     /gene="phrB"
                     /locus_tag="L2X49_003586"
     CDS             complement(11853..13271)
                     /gene="phrB"
                     /locus_tag="L2X49_003586"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBN4798607.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13268..13777)
                     /locus_tag="L2X49_003587"
     CDS             complement(13268..13777)
                     /locus_tag="L2X49_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBN4798608.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT         3349 a         3731 c         3413 g         3403 t
ORIGIN      
        1 taagaaaaac taaaaacatt cgacaatcaa ataagatatt cttgcaccga tatacttttt
       61 tcttttctgt taaaggactc cgttatgttt aaaggacaaa aaacattggc cgcactggcc
      121 gtatctctgc tgttcacagc acctgtttat gcggctgatg aaggatccgg tgaaattcac
      181 tttaaaggtg aagttattga agcaccgtgt gaaatacatc aggatgatat tgataaagag
      241 gttgaactcg gtcaggtgac caccagccac attaatcagt cacatcacag cgatgccgtt
      301 gctgtcgacc tgcgcttagt caactgtgat ctggaaaact ccagcaacgg ttccggtggc
      361 aagatttcta aagttgcagt cacctttgat agctcagcga aaaccaccgg cgcagatccg
      421 attctcaaca acaccagcac gggtgaagcc accggcgtcg gcgtacgttt aatgaataaa
      481 gatcaaagca atatcgtttt aggcaccgct actccagata tcgacctggc tccgacctcc
      541 agcgaacaaa cgctgaattt ctttgcctgg atggaacaaa ttgatcaggc tacacctgta
      601 acgccaggcg cagttacggc gaatgcaacc tacgtgctgg attataaata agtattactt
      661 tggcggcgct atcgccgcct ttcataaaat atttatcagg tatggacacc gtgaatattt
      721 atcgactctc ttttgtatcc tgcctggtcg tggcgatgcc ttgcgcattg gcggtcgaat
      781 tcaaccttaa tgttctcgat aaatcaatgc gcgaccgcat tgatatttca ttattaaagg
      841 aaaaaggagt cattgctccc ggtgagtatt ttgttagcgt tgcggtaaat aataaccaaa
      901 tcagtaacgg gcaaaagatt aactggcaca aaaatgacga taaaaccatt ccgtgcatca
      961 atgatttact ggtcgataaa tttggcttaa aacctgaagt ccgtcagtcg ttaccattga
     1021 taaatcagtg cgtcgatttt agctcccgac ctgaaatgct cttcaatttc gatcaagcca
     1081 atcagcaact aaatatcacc attccgcaag cctggctggc gtggcactca gaaaactgga
     1141 ccccaccctc cacatggaaa gaaggtgtcg ccggtgtcct gatggattac aacttgtttg
     1201 ccagcagcta ccgcccacag gatggcagca gcagcactaa cctgaacgcc tacggtacca
     1261 ccggaattaa cgccggggca tggcgcttac gtagtgatta tcagttgaat cagactgata
     1321 gcgatgataa ccatgaacag tcaggcggaa tatcgcgcac ctatcttttt cgtccattac
     1381 cgcaattagg ctctaaatta accctcggcg aaacggattt tagttccaat attttcgacg
     1441 gtttttctta taccggcgcg gcactggcaa gtgacgagcg aatgttgcca tgggaactac
     1501 gcggctacgc cccacaaatt agcggtattg cacagaccaa tgccacggtg acgatcagtc
     1561 aatcaggccg cgtcatttac cagaaaaaag tcccaccagg cccatttatc attgacgacc
     1621 ttaatcagtc tgttcagggc acactggatg tcaaagtgac ggaagaagat ggtcgggtga
     1681 acaatttcca ggtttcggca gcatcgacgc ccttcctgac tcgtcaggga caggttcgct
     1741 ataaactggc cgcgggtcag ccacggccct ccatgtcaca tcaaactgaa aatgaaacct
     1801 tttttagcaa tgaagtttcc tgggggatgc tgtcaaacac ctcgctgtac ggcggcctgc
     1861 tgctttctgg tgatgactac cattctgccg caatgggtat tgggcaaaat atgctgtggc
     1921 ttggtgcgct gtcgtttgat gtcacgtggg ccagtagcca ttttgatact cagcaggacg
     1981 agcggggctt aagctaccgt tttaattaca gcaaacaagt ggatgccacc aacagcacga
     2041 tttccctcgc cgcttatcgt ttctccgatc gtcattttca cagctacgcc aactatcttg
     2101 atcacaaata caacgacagc gatgcgcagg acgaaaaaca gacgatcagc ttatctgtgg
     2161 gccaaccgat taccccacta aacctcaatc tttacgccaa cctgctacat caaacctggt
     2221 ggaatgcaga cgcctccacg accgccaaca tcacggcagg ttttaatgtt gatattggtg
     2281 actggagaga tatctctatt tcgacgtcat tcaatacaac ccattacgaa gataaagatc
     2341 gcgacaacca gatttacctg tcgatttcgc tccccttcgg taacggtggt cgggttggct
     2401 atgacatgca aaacagtagc cacagcacca cacaccgcat gtcgtggaac gatacgctgg
     2461 atgaacgtaa tagctggggc atgtctgccg gactgcaatc cgaccgtccg gacaatggag
     2521 cccaggtgag cggtaactat cagcatctga gttcagcggg tgagtgggat atttctggta
     2581 cctatgccgc caatgattac agttccgtca gcagcagctg gagcggttct ttcaccgcaa
     2641 cccaatatgg tgcagcgttt catcgccgca gctccaccaa tgaaccacgc ctgatggtca
     2701 gcaccgatgg cgtggcagat attccggtcc agggcaatct cgactacacc aaccattttg
     2761 gcattgcggt ggtgccgttg atttccagtt accagccttc caccgtggcg gtgaacatga
     2821 atgacttacc cgacggcgta acagttgcag aaaacgttat caaagaaacg tggattgaag
     2881 gcgcgatagg ttacaaatca ctggcttccc gttccggtaa agacgttaac gtcatcattc
     2941 gcaacgccag cggtcagttt cctcccctcg gagcggatat ccgccaggat gacagcggca
     3001 ttagcgtggg gatggttggc gaggaaggac atgcctggtt aagcggagtc gctgaaaatc
     3061 aaaagtttac cgtggtctgg ggtgatagcc agcattgctc gctccatctt cctgaacata
     3121 tggaagacac cgcaaatcgc ctgattttac cttgtcatta attaaggaaa tgaaaatgac
     3181 atttatgaaa ggactacccc ttttgttgtt ggttgccagt ctgtgcagcc acgctgcact
     3241 acaacccgat cgcactcgta ttgtgtttaa cgctaatgat aaagctacca gtctgcgggt
     3301 ggataatcgt agcgataaac tcccctacct cgcttattcc tggctcgaaa atgaaaaagg
     3361 ggagaaaagc gatgatcttc tggtcgccct gccgccgata caacgtctgg aaccgaaagc
     3421 aacaacgcag gtacggatag taaagcaagc ctcgaccaca aaattaccgg gcgatcgcga
     3481 aacgctattt ttctacaata tgcgtgaaat tcctcccgcc ccggaaaaaa acagcgacca
     3541 tgccgttctg caagtcgcca ttcagagtcg tatcaaggtg ttctggcgac cggctgcatt
     3601 acgcaagaaa gcaggagaaa aggtcgaact gcagttacag gtcagccagc agggcaacca
     3661 actgacactg aaaaatccta ccgcgtatta tctgaccatt gcctacctgg gacgaaatga
     3721 aaaaggcgtt ctccccggct tcaaaaccgt gatggttgcg ccttttagta cggtcaacac
     3781 aaacaccgga aattatagcg gcagccagtt ttatctcggt tatatggatg attacggcgc
     3841 gttacgcatg acgacgctca actgcagcgg acaatgccat ttacaagccg tggaggcgaa
     3901 gaaatgagtg ctggcaaggg agtgttgctc gtcatctgtc tgttatttct gccgttgaag
     3961 tctgcgcttg ccctgaactg ctactttggt tcatccggtg gctcggtaga aaaatcagaa
     4021 gcgattcaac cttttgctgt accaggcaat gccaaactcg gcgataagat ttgggaatct
     4081 gacgatatta aaattcccgt ctattgtgac aacaatacca acggcaattt tgaaagtgag
     4141 catgtttatg cctgggtaaa cccctacccg ggggtgcagg atcgctatta tcaactcggc
     4201 gtaacttaca atggcgttga ttatgacgcc aaccagggaa aaagccgcat cgacactaac
     4261 cagtgtattg acagtaagaa cattgacatt tatacccctg agcagattat cgcgatggga
     4321 tggcagaaca aaatttgctc gggtgatccc gctaatattc acatgtcgcg tacctttctc
     4381 gctcgcatgc ggttatacgt caaaatacga gaaatgccgc cgcatgatta tcaaagtacg
     4441 cttagcgact atatcgtcgt gcaatttgac ggtgccggta gcgttaacga agatcctact
     4501 gcccaaaacc tgaaatatca tattaccggt ctggaaaaca ttcgcgtgct ggattgcagc
     4561 gtcaattttt ccatttcacc ggaaacacag gtgattgatt ttggtaaatt taatttgctg
     4621 gatattcgtc gccacacaat gtcgaaaaca ttcagtatta agacgacaaa atcacaaaat
     4681 gaccaatgca ccgacggatt taaagtcagt tcctcctttt ataccgaaga aacgttggtt
     4741 gaagaagata aagcattgtt gattggtaat ggtctgaagc tacgtttgct ggatgaaaac
     4801 gcctcgcctt ataccttcaa taaatatgct gaatacgccg atttcaccag cgacatgttg
     4861 gtctacgaaa aaacctatac agctgaactt tcgtccattg caggtacccc catcgaggct
     4921 gggcccttcg atacagtggt gctttttaaa attaactata actgatttgc catgatgccc
     4981 tttgcagccg gaaagggcat ctctttcaga aaattgctga ttttcccctt ttccttgatt
     5041 gctgttattt tttcctggcc cggctttctt tatgctgtat cgatacacca aagagaaaat
     5101 aatgagggag cgaaggatgc cagttttgca gtggggaatg ttatgtgtgc tatcacttct
     5161 cctttctatt ggtttcctcg cggtacatct cccggcggcg ctattacttg ggccgatgat
     5221 cgccgggatc atcttcagta tgcgcggaat tactctgcaa cttccccgct ccgcttttct
     5281 cgccgcgcag gccattcttg gctgcatgat tgcgcaaaac ctcactggtt cgattctcac
     5341 cacgctggcc gccaactggc cgatcgtgct tgctatttta ctggtgacgc tgctttccag
     5401 cgccatcgtg ggttggttat tggtgcgcta tagctcactg cctggaaata ccggtgcctg
     5461 gggttcttct ccaggcggcg cagcggcaat ggtcgctatg gcgcaagatt acggcgcaga
     5521 tattcgcctg gtggcgttta tgcaatatct gcgagtgctg ttcgtcgcgg gggccgctgt
     5581 tctggtcacc cgcatgatgc tgggtgataa cgctgaagcg gttaaccagc aaatcgtctg
     5641 gttcccgcca gtgagcatta atctcctgct tacgattttg ctcgcagtcg ttgccggtac
     5701 ggcgggatgc atgctgcgtc ttccttccgg cacgatgctc atcccgatgc tggcgggcgc
     5761 agtgctccag tctggtcagc tcatcaccat cgaactaccg gaatggctgc tggcgatggc
     5821 gtatatggca attggctggc ggattggtct tggtttcgat aaacaaatct tactgcgggc
     5881 actacgccca ctaccgcaaa tcctgctgtc gatttttgct ctgctggcta tttgtgcagg
     5941 tatggcgtgg ggactgaccc ggtttatgca tattgatttt atgactgcct acctcgccac
     6001 cagtcccggc gggcttgata ccgtggcggt catcgccgca gggagcaatg ccgatatggc
     6061 gcttattatg gcgatgcaaa ccctgcgcct gttcagtatt ttgctgacgg ggccagccat
     6121 tgcgcggttt atttcaacct atgcgccgaa gcggtcggcc tagtgctggc agcctggaca
     6181 ccagtaaaac gggcgcgagg acaacgtggt tttctcaatg atgccgccac agcgttcgca
     6241 cagttcgcca tcacgatgaa aaaccttaaa gcgaaacagc gccccatgat gcttattctc
     6301 atccacctgc ccccgcgtag cgtaagaaaa ccgaggaatc tccagtaacg cgtgtgcgag
     6361 tgcatccagt tgcgccgcat tgagatcttt cgctttatga tttccagtca ccccaacctg
     6421 ccagaggatc tccacccgca aataattgcc aagcccagcc agaaacgcct gatcgagcag
     6481 taatcctgca aactgacggt tacgaaagcg cggtgacaat aaccgttcct ttaccacctc
     6541 cggcgtcaga ttcggatcca gcacatcggg accaacgcgt tgtaaaaacg gatgcgtggt
     6601 cagttgttcc ggggtcaaca tctcaatatc cgaggcgcta taaagcagaa tggttttgtc
     6661 agccgtttgc agttttaccc gcaatactcg cgtggtctgc ggctcttcgc cggtatcaac
     6721 cacgcgccag acgccgtaaa gctgattatg gctgtagagc gttaagtcgt tggaaaaatg
     6781 agttaacaac gccttaccac gcgtttccac atgggtaacg tgttgaccga taagttgtga
     6841 ttgataagtt tttaactgcg ggaaggcaaa ccagacatca gttagtggtt tgcctttgat
     6901 cgccgcctcc aggttatccg ctgcacggcg gatctccggg ccttcaggca tggtgatatc
     6961 cttttttcat tctttattaa gcggggttat gctgcgacaa caatcccctt tttggcaaat
     7021 gtagctcgta atcggcgggc gaacgccaga gcgtgctcgc cgtcgccatg caggcagacc
     7081 gtttgcgccg cgaccgttgc ccattcgccg gtgatacttt tgactctgcc gtgttgcacc
     7141 atctccagcg tttgcgccag cgcctgttgt tcgttttcaa tcaacgcgcc tcgctgactt
     7201 cgcggcacca gcgagccgtc agcctgataa ccgcgatcgg caaacacttc ctcgcgcgtt
     7261 gtcaggccat attgcttgcc tgcacgaatc agctcgcttc ctgccagccc aacgagaatc
     7321 aatgccggat cgcaagcgta taccgctctg gcgatggcgt ctgccagttg tgcttctttc
     7381 gccgcctggt tgtacaacat gccgtgcggt ttgacatgac gcattacgcc gccttgcgca
     7441 cgggcaatcg ttgccagcgc gccaatttga tacagcgtct gggcgtaaac ggtttctggc
     7501 ggcagctgca tggcgctgcg accaaaattt tccctgtcgg gaaaactcgg gtgagcgcca
     7561 attgcaacac cattttttat tgcttcacgc acgcaagcat gcatggtttg cgcatcgcct
     7621 gcatgaaatc cacaggcaat attggcagag gaaaccagcg ttaatagctc tgcgtcgctg
     7681 gcgcagcctt cgcccagatc ggcgttcagg tcaattttca ttgtgcagcc gccacgctaa
     7741 ttgttcaaaa taacgttgct gatcttgccg cgctttcagt gcctcttcca gtgaacactg
     7801 gacaaaatga atcggctgac cgagcggaat ttgcgccaga tggtacatat cagcctcaat
     7861 gatgcaggca atacgcgggt aaccaccggt ggtctgtgcg tcgttcatca acacaatcgg
     7921 ctgcccgtta tgtggcacct gcaccacgcc cggtaacaaa ccgtgagata acagttcgcg
     7981 atcggtggtg cgttttaaaa tttgcccctg taagcgatag cccatgcggt tactttgcgg
     8041 gctaagctgc cagggcgaac gccagaatgc atcctgcgag gcgcgatcga actcatgata
     8101 ttccggcccc ggcaaggcgc gaatgcggtt gccccacagc agctgtttaa cgccctgcgc
     8161 ttccataaaa tcacgcttcg atttgccaat cgggagtcgg tcaccatcct tcagtaaacg
     8221 gccttccagc ccgccaatcc ccacgttgag atcggtgctg catgacccca ttaccggcgg
     8281 aacatcaata ccacccgcga ccgccagata actgcgcatc ccgtgctgcg ggcgtttaag
     8341 cgttaaacgc tggcctgctt tcatcggcaa tcgccagccg gtccagacgg cattatcatc
     8401 cagccgcgct tcgcaaccgg cacccgtcag agcaaaccac ccatcagttt cgaactcaac
     8461 agttaactga ccgagcgtga tctccagcgc gggggcattg gcgtcattac ccaccagtag
     8521 gttagcaatg cgtaacgcgg gcatatccag tgcgccgcag tggctgatac ccgactggcg
     8581 aaaaccgtga cgaccgccat cctgcacagt ggtatacatg cccgcacgaa taatcttcag
     8641 catactccct ccttctgcgg tacaaagcgc acgctgtctc ccggacgtaa taagatgggt
     8701 tcgtcacgcg ccggatcaaa caggctgagt gaggtatgac caatcaactg ccagccaccc
     8761 ggcgttgcca gcggataaac accagtctgc ggcccgccga tcccgacaga acctgccgga
     8821 acgagtaagc gcggttcagc gcgccgtggc gtgtgtagtt gttccggcaa actcccgaga
     8881 tacgggaagc ccggttgaaa acctaaaaac cagaccacgt attccacgga ggagtgcaat
     8941 tcaacaacct gtttttcgct caacccgcaa tgcgccgcga ccaccgccaa atccggtccg
     9001 cctgcaccac cgtaaaccac cggaatttca ataaagcgag actcaggttc cagggcctcg
     9061 ctctcctccc accagcgttg caaacgctct atggcatcca gcgccagcga ctcaggatta
     9121 cgcaaaatca ccgtaatatt gttcatgccg ggaatggctt caaccacatt cggcatatcc
     9181 accagacgct gcgccagtcg ccagatccgt ttctggctag ccagcgtcac cggcggttcc
     9241 agttccagca ctaccgccgt ttcacctatc agataacaac gcgctcgttg cactttcgat
     9301 ccctctgatt attcgttatg caggattagg aatatcaata aaggtcacat caagatcggt
     9361 attttcattc agccactcgc tcaatgcgcg aataccacca cgttcagtgg catggtgacc
     9421 tgcagcataa aaatgcaatc cctgctcgcg ggctgaatga atggtctgtt cagaaacttc
     9481 gccagtaata aaagcatcca cgccaaaacg cgcggcgcta tcgataaaac tttgcccgcc
     9541 gcccgtgcac caggcgacgc gctggaccac ctcaggtccg gtatcgccac accataacgg
     9601 cttacgtccc agacgcgctt caatccagga ggctagttcc agtcccggca caggcatggt
     9661 cagctctccc cacggcacca acggctcaat ttcgcccatg accgtgatcc ccagtaacgc
     9721 cgccagctgt gcgttattgc ccagctcagg atgtgcgtca agcggcaaat gccagccata
     9781 caggttgata tcattcgcca gcaacgtttt taaacggtta cgcttcattc cgcgaatgac
     9841 cggagactct cctttccaga agtagccatg atgcacaatg accgcatccg cgcccagacg
     9901 cactgcctca tcgagcaaag cctggctggc ggtgacaccg gtaacaattt tttgcaccgt
     9961 ctctttgcct tccacctgca aaccattcgg cgcgtaatca ctaatcgccg cgctgttcag
    10021 tttttcgtta atcagttgtt ccagttcggt gtttttcatc ttcgcgcctt ccgtattctg
    10081 atagctacac cgttaacata gcgttacctc gcctgttcgt cgatagccaa tcttttgaca
    10141 tattaagaaa caaacccgac gccaaaagac gcaaattgtt tatatcatca cgcttcagct
    10201 aatgtatatt tatgcaatca gacagcatat tgcataaata atcagatctg tatcaaagtt
    10261 ttaactttaa ggaaacacaa gcacgccgcg caactattac gtgacgttgc cgtcaattgt
    10321 ggaattatta tgaataaaca cgcatcacaa ccgcgcgcta tttactatgt cgttgcgctg
    10381 caaatctggg aatattttag cttttacggc atgcgtgccc tgctgattct ctatctcacc
    10441 aatcaactaa aatacaacga tactcacgcc tacgagttat ttagcgccta ctgttcgctg
    10501 gtgtatgtca cgccaatcct cggtggcttt ttggcggata aagttctcgg caatcgcatg
    10561 gcggtgatgc tgggggcgtt gttgatggcg attggtcatg tggtgctggg tgccagtgag
    10621 atccatccgt cattcctcta tctgtccctg gcgattatcg tctgcggcta tggcctgttt
    10681 aaatctaacg taagctgtct gctcggcgag ctgtatgagc caaccgatcc gcgtcgtgat
    10741 ggcggtttct cgctgatgta tgcggcgggt aacgtggggt ctattatcgc acctatcgcc
    10801 tgtggttacg cccaggaaga gtacagctgg gcgatgggct ttggcctggc ggcggttggc
    10861 atgatcgcgg gtctggtcat tttcttatgt ggcaatcgtc atttcactca tacccgcggc
    10921 gttaacaaaa aagtactgcg tgcgacaaac tttctcctgc cgaactgggg atggctgctg
    10981 gttctgctgg tggcaacgcc tgcactgatt accgtactgt tctggaaaga gtggtcggta
    11041 tacgccttaa ttgtcgcgac tattattggc ctgggtgtac tggcaaaaat ttatcgcaaa
    11101 gcagaaaacc agaaacagcg gaaggagctg gggctgattg tgacgctcac cttcttcagt
    11161 atgttgttct gggccttcgc acaacagggc ggtagctcga ttagccttta tatcgaccgc
    11221 ttcgttaacc gcgatatgtt tggttatacc gttccgaccg cgatgttcca gtcgattaat
    11281 gccttcgcag ttatgctgtg cggtgtgttc ctggcgtggg tggtaaaaga gagtgtcgcg
    11341 ggtaatcgta ccgtgcgcat ctgggggaaa tttgctcttg gccttggcct gatgagcgcc
    11401 ggattctgca ttctgacctt aagcgcccgc tggtccgcaa tgtatggtca ctcttctctg
    11461 ccactgatgg tattaggcct ggcggtgatg ggctttgcgg aactgtttat cgacccggtt
    11521 gccatgtcgc aaattacgcg cattgaaatc cccggtgtga ccggcgtatt aaccggcatc
    11581 tacatgctgc tttctggcgc gatcgcgaac tatctggcgg gcgtgattgc cgatcagaca
    11641 tcgcaggctt cgtttgatgc ttccggggcg atcaactact ccatcaatgc atatattgaa
    11701 gtatttgatc aaattacctg gggcgcactg gcgtgtgtag gactggtaca gatgatttgg
    11761 ctgtatcagg cgctgaaatt cagaaaccgc gcgctggcgc tggaatctta ataggccagc
    11821 accatacctg ataaaacgct cgctgcgttt tatcaggctc ctttcctcgc ttcttcgtac
    11881 gctgccagcg ttcgcagcct tgcctctttg tgatcaacta tcggttgcgg ataatccagc
    11941 atcacacctg ctttctgcgc ccacttccac ggctcatgca ccgctttccc tggtacatcg
    12001 cgcagctccg gtaaccatcg gcggataaac tcaccttcgc gatcaaattt ctcgccctgg
    12061 gttgtcgggt tgaaaatacg aaaatacggc gctgcatcag tacccgtaga cgccgcccac
    12121 tgccagccac cgttattggc tgccaaatca ccatcaatca gctgcgacat gaaatatcgc
    12181 tcgccttcgc gccagtcgat caacaaatct ttcaccagaa aactggctga aatcatccgt
    12241 agccggttat gcatccagcc agtgctgtta agctgacgca tggcggcatc gacaatcggg
    12301 tatcccgttt tgccttcctg ccaggcctgt aaatgtgcgg gattgctctg ccactgcaca
    12361 cgatccgtcc aggcaataaa cggacaatgt ttacacaacg aggggtaata cgtcatcaga
    12421 tgacggtaga actcgcgcca gatcagctca ctaagccaga cactaccggc cccaccgtcc
    12481 agcgcctgcg gctgttccgc caacaagcga tgcaaacact ggcgaggcga taacccgccc
    12541 gttgccaggc tggcggacaa acgactggtg ccttccactg ccggaaaatc tcgttgttgc
    12601 tcatattctc cggcaccgtt ctggcaaaac tggcgtaatt gcgcaatcgc cgctttttct
    12661 tccaccggga aatgcgcagt atcgaaagac tgacgaggat aattcagcgt aatggatggc
    12721 gagggctcta tcgatccgct actacgaact tttggcgcag cgacgcactc cggcatccct
    12781 tcccgcagcc gtttcagcca ggcattctta aaaggcgtaa agactttgta catctcgtga
    12841 ttaccggtca tcaccgcgcc aggcggcagg atcacgctgt catcaaatcc ttcacacacc
    12901 acgttacgca gcgctctttc aacttccaca tcccgcgccc gctcattcac ttcatactga
    12961 tagttataaa acaggtgggt aacgctgttt tccgcgcaca cctgtttaac tatttcgaca
    13021 ctggcgacaa agtcatccac ttcacggaac aataaaggaa tacctttttc cgcaagcgct
    13081 atttgtagcc cattcagttg agcattgata agttcagcct gacgcggcga catgttatgc
    13141 gtcgcccact ggcgtggtgt agcgatatac aacgccagca cgcgtgcaga cgaattgcgg
    13201 caggcggcag ccagtgcgag attatcgtgc agacgtaaat cctggcgaaa ccagaccaga
    13261 tgggtagtca taaaactcct gaatggcgca agttaatcac acgcggccaa agttcgaaat
    13321 aacgctgccc tgaaagccag gcgtcaggat aaagcgccat atagtgtttg atacgcatca
    13381 gcggcgcaag aagtggttgc tcgccacggc gataactgtc gataagcgca gccagctgct
    13441 ggcgttctgt ggaatcaata tgcgggcgaa aataaccctg aacgtgcatc aaaacattgg
    13501 tgtgatcgcg cgggttggcg gggtgagaaa gcaaaacaac tacgcgttgg cggtactggt
    13561 tgtaatagtc atccagattt tgccactggt gaatatcagc cacgaacggg ccaagtttgc
    13621 ggtactccgg ctgagaatgc gccagaaaga cgagtttata gcggctgtga taatcgagaa
    13681 gcgccccgcg cgttaagcca tgttctttca gtcgatgtaa ctcatgaagg gcaaagatac
    13741 ggattagggt gctgtcatca aatcgttgta gattcattac tgctcctgaa cggtataaag
    13801 gagtaagtgt agacgatggt aaatgaattt tatggggcag agtttttgcg gggaagtatt
    13861 ttgttggtaa aaatattatc aacttcatct gagcga
//
LOCUS       DAFNBJ010000126        13865 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000126 DAFNBJ010000000
VERSION     DAFNBJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.126"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..800
                     /locus_tag="L2X49_003588"
     CDS             <1..800
                     /locus_tag="L2X49_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBN4798609.1"
                     /translation="VSFLMSFSSRTATRDRLHIFTTNYDRIIETGAEIAGLRLIDRFV
                     GTIAPIFRSSRLEVDYHYNPPGIRGEPRYLEGVARFTKLHGSLDWYATEGAIRRFGLP
                     FGASSVEPFLQVETAGTANYQQLMIYPNSVKDRETSEYPYVELFRDLASATCRPNSTL
                     VTYGYSFGDEHINRLIIDMLTIPSTHIVIIAYGDPLGRIMRFINDSGRKAQISLLLGD
                     HFGDLKKLVDFYLPKAAIDRSSIRMAELLKSRGLYRATKPNNSEDAE"
     gene            797..2869
                     /locus_tag="L2X49_003589"
     CDS             797..2869
                     /locus_tag="L2X49_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBN4798610.1"
                     /translation="MNSFPIEQGEQLRVGTVDFVSPNEIRALLDIDSPDSVALNAGTP
                     RNFPRVNSYVLVSCDNGYLVGQIEWLAVEHSPYPKQRDIQDFGLVNLPFPRKKISLNP
                     VGNLKRFSKDGTDYFIFQRGSESFPSIGSAILLPTDLQLRSIVESGNNRRVIIGQSPL
                     ANNANVAVDPDRLFGRHIAVLGNTGSGKSCSVAGLIQWSLEAAMESEIKPNARFIILD
                     PNGEYTRALGPTTKFKGRVFKVEAEDGENQLQVPSWFWNSSEWASFTQASPKAQLPLL
                     KRSLRAMRNEEFDLQTNLDVEVKKYLGTILVSLKADKSKGAAALNDFPGAKNLLAKIN
                     TWRQSLEEYKERLVTTHPELDKLIVSIQDFCVQREGRYPDYNAKVSAVDNIIDGMLSS
                     FQSLGGNECELLPKNEDIPVPFDGGNLVSYLEALAQENGSEQYVEYLVARIRTMLADT
                     RMKPITNDSEHRVDLANWLETYIGKDGTGDDDSCVSIIDLSLVPTEITHLVTAVISRI
                     VFESLQRYRRLYNKSLPTVLVAEEAHTFIKRYRDDSENQDVAAVCCQVFEKIAREGRK
                     FGLGMVISSQRPSELSPTVLSQCNTFLLHRISNDKDQEQVHKMVPDNLRGLLRELPSL
                     PSQHAILMGWASELPVLVKMKNLTKEQQPHSDDPDFWDVWTRKYADGKLVERTVDWEA
                     VVKEWQQK"
     gene            3545..3874
                     /locus_tag="L2X49_003590"
     CDS             3545..3874
                     /locus_tag="L2X49_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBN4798611.1"
                     /translation="MFSVVYHPEAREEATALPVKIRVKFDRLIGKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            3855..4136
                     /locus_tag="L2X49_003591"
     CDS             3855..4136
                     /locus_tag="L2X49_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4798612.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVIALY"
     gene            4414..5394
                     /locus_tag="L2X49_003592"
     CDS             4414..5394
                     /locus_tag="L2X49_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HBN4798613.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNNEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQPGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGRVKTLKQHPRKNKTAINIEYMKASIRARVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
     gene            complement(5546..6514)
                     /locus_tag="L2X49_003593"
     CDS             complement(5546..6514)
                     /locus_tag="L2X49_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HBN4798614.1"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQARYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
     gene            6869..7516
                     /locus_tag="L2X49_003594"
     CDS             6869..7516
                     /locus_tag="L2X49_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBN4798615.1"
                     /translation="MNVIQPRMLSVFDFDGTLTRHDSFIPFLRFAFGNRAFSRRIVSM
                     ALPTLRCMGKQMTRDELKETLITTFLTGVEEKWVSSKAEEFCQLYWHRLMRPAGLIAV
                     ANEVTSGAIVTLCSASPEIVLRPFAEKLRIKLIGTQLETKDGILTGRISGHNCRCSQK
                     IIRLEKEYGPLTDYHLRAWGDTRGDYELLSAATEPHWRHFHQGFRRKLPSKLIIR"
     gene            complement(7853..9094)
                     /locus_tag="L2X49_003595"
     CDS             complement(7853..9094)
                     /locus_tag="L2X49_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBN4798616.1"
                     /translation="MQLQSGQNIPLHTDTLTLRLDYPPSPGFHSTPETCLFMLNAEGK
                     VTGDADFIFYNNLSAAGGSVKLNPGHQHAVVHFALDQIPDTIQKIAITMVIDGSDTIS
                     GLAQLKLSAESQATFFVDLNGRTEKAVIMGEVYRYQGNWKLRALGQGFNGGLEPLAVS
                     YGVDVEPAAEVVPQPARISLEKKLEGKAPALISLAKKASISLAKHKLDTVEARVAFVL
                     DASGSMTGQFKKGHVQAVLDRIAVLSVQFDDDGTMDVWGFAERHKKYPDVTLDNLDGY
                     IAAIQNTGKRSAWEILPGLGGTNNEPPVMNEVIDYFKDSTLPVFVVCITDGGISKTRE
                     IKEAIRRSANYPVFWKFVGLGGSNYGILERLDTFSDRRVDNSNFFAIDNFAHIKDEEL
                     YEKLLEEFKDWLGLAKKEGII"
     gene            complement(9179..9754)
                     /gene="terE"
                     /locus_tag="L2X49_003596"
     CDS             complement(9179..9754)
                     /gene="terE"
                     /locus_tag="L2X49_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance cAMP binding protein TerE"
                     /protein_id="HBN4798617.1"
                     /translation="MAVSLVKGGNVSLTKEAPSMNVALVGLGWDARVTDGQAFDLDAS
                     VFLVGENGKVLSDSHFVFYNNTTSPDGAVQHQGDNRTGEGDGDDEQVKIDLTKVAADV
                     KKLVFAVTIHEADSRKQNFGMVSNSFMRVVNNDNGAEIARFDLSEDASTETAMIFGEL
                     YRHGAEWKFKAVGQGFAGGLAALATQHGINI"
     gene            complement(9841..10419)
                     /gene="terD"
                     /locus_tag="L2X49_003597"
     CDS             complement(9841..10419)
                     /gene="terD"
                     /locus_tag="L2X49_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HBN4798618.1"
                     /translation="MSVSLSKGGNVSLSKTAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLAANGKVRGDADFIFYNNLKSADGSVTHTGDNRTGEGDGDDESLKIKLDAVPGDV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(10458..11498)
                     /gene="terC"
                     /locus_tag="L2X49_003598"
     CDS             complement(10458..11498)
                     /gene="terC"
                     /locus_tag="L2X49_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HBN4798619.1"
                     /translation="MVSTHIGFPAETVTVFVALSVGAIFIDLFMHRHDKPISLKSAAL
                     WSVFWVVVAMAFAGFLFVHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFSVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTGLLSLGPYVELIFAVVVAWTAVMMLKGGGDDDE
                     IEDYSQHLAYRLVKRFFPIWPKLSGHAFLLTQKEVDAELAKPENKDVSVGRLKKAALY
                     ATPLMLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLSQLEKAVIVLLFFIAFKLGLNATDHIWHHGYSLSATTSLYVVLGVLALGILASVI
                     FPEKPEDENKES"
     gene            complement(11522..11977)
                     /gene="terB"
                     /locus_tag="L2X49_003599"
     CDS             complement(11522..11977)
                     /gene="terB"
                     /locus_tag="L2X49_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerB"
                     /protein_id="HBN4798620.1"
                     /translation="MSFFDKVKGALTSGREELTRQVGRYKNKKFMQGTVAVCARIAVA
                     SDGVSSEEKQKMIGFLRSSEELKVFDTAEVIEFFNKLVTSFDFDLEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDDDEKSAVREIARSLGFDPAEFGL"
     gene            complement(12000..13151)
                     /gene="terA"
                     /locus_tag="L2X49_003600"
     CDS             complement(12000..13151)
                     /gene="terA"
                     /locus_tag="L2X49_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance system protein TerA"
                     /protein_id="HBN4798621.1"
                     /translation="MNLTPGGNAPVPAQELRVRVISGGPVDASAFRLFADGKVRGDSD
                     MVFYGQPRNEDGSISFSTEGTNSVFTVDLSRLKPDVQKVAFTVTCDGSHTVSSLNHLS
                     IQIESGNTSLISGQVELSGRQEAALILGEVYRRNGDWKFRFIAQGFNGGLKPLAEYYG
                     VNVADEPAPVTPPPAPPAPPKVSLSKVSLTKEKPAISLAKKDNFGEIRINLNWHRGSG
                     SGGLLGGMFGSNKAIDLDLGAFVELADGYKSVVQALGNAFGNYHSEPYVQLQGDDRTG
                     EALDGEWLHINGREWKEIRQVLIYAFIYQGVPSWDKTDGVVTLHVPEQPPIESRLTEG
                     DNKRTLCAIARLVNENGSIRVERINQYFRGQEEMDRAFGWGFRWSRGSK"
     gene            complement(13148..13732)
                     /locus_tag="L2X49_003601"
     CDS             complement(13148..13732)
                     /locus_tag="L2X49_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance TerZ family protein"
                     /protein_id="HBN4798622.1"
                     /translation="MVSLSKNQTVSLSKQSTDLSQLQFGLGWDPVKKKGLFGSFFGGN
                     DSIDLDAGCVLLDVAGNKIDTIWFRKLKSSCGSVVHSGDNLTGEGDGDDEIIHVNLLK
                     LPANVEYLAFTVNSFRGQSFNDVDNAFCRVVDQTNKELARYQLTEQGPHTGIIIASLR
                     RNNGHWDFTAHGKASGGRTIDDMQRDIISTVVKA"
BASE COUNT         3690 a         3343 c         3286 g         3546 t
ORIGIN      
        1 tagtgagttt tttgatgagt ttttcaagtc gaacagcaac tagggatcga ttacatattt
       61 ttacgacaaa ttacgacaga ataattgaaa cgggcgctga aatagcaggt cttcgtttaa
      121 ttgacagatt tgtcggtacc atagcgccga tttttagatc ttcgcgatta gaagtggatt
      181 atcattacaa tccaccaggt attcggggtg aaccaagata tttggaaggt gtggcgcgct
      241 ttactaaatt acatggttca ttggactggt atgctactga aggggctata aggcgttttg
      301 gattgccatt tggagctagc tcggttgaac cctttttaca agtggaaaca gccggaactg
      361 caaattatca acaattaatg atttatccaa attcagttaa agaccgtgaa acatctgaat
      421 atccttatgt tgagctattt agggatttag catctgcaac atgtcgtcct aatagcacct
      481 tggtaacata tggttatagt tttggagatg agcatataaa tcggcttata attgatatgt
      541 tgacaatccc ctcaacgcat atagtcataa ttgcttacgg tgatccgctt ggaagaatta
      601 tgcgttttat aaatgatagt ggaagaaaag ctcaaatatc tttattgctg ggagatcatt
      661 ttggagatct aaaaaaatta gttgattttt atcttcctaa agccgcgata gacagatcat
      721 caattcgtat ggctgagcta ttaaaatcac gaggtttata tcgtgcaaca aaaccaaata
      781 atagtgagga tgctgaatga actcattccc cattgaacaa ggtgagcaac tcagggttgg
      841 cactgtcgat tttgtttcac ccaacgagat tagggcacta ttagatatcg actcgcctga
      901 ttctgtcgct ttgaatgcag gaacacctag aaattttcct agagtaaaca gctatgtatt
      961 agtctcatgt gacaatggtt acttagttgg gcaaatagag tggcttgcag ttgagcattc
     1021 gccttatccc aaacaaaggg atatccaaga ttttggttta gtaaatctgc cattccctcg
     1081 aaaaaaaata agtttaaatc cggtagggaa tcttaaacgt ttttcaaaag atggtactga
     1141 ttattttata tttcaacgcg gttctgaatc atttccttct attggttcag ctattttact
     1201 accaacagat ttacagctta gatcaattgt cgagtctggt aataatagac gggtgattat
     1261 tgggcaaagc ccacttgcta acaatgctaa tgtggctgta gatcccgaca gattatttgg
     1321 tcgtcatata gcagtacttg gtaatacggg tagtggtaag tcttgttctg ttgcagggtt
     1381 aatccagtgg tcactcgagg ctgcaatgga atcagaaata aaaccaaacg caagatttat
     1441 tatattagac ccaaatggcg agtatacacg tgctttgggg ccaactacaa aatttaaagg
     1501 cagagtgttt aaggttgaag cagaagatgg tgaaaaccaa ttacaggtac cttcatggtt
     1561 ttggaatagt tcagaatggg catcattcac gcaagcaagt cctaaagcac aacttccatt
     1621 actaaagcgc tctttgagag caatgagaaa tgaagagttt gatttacaaa cgaatttaga
     1681 tgtagaagtt aaaaagtact taggtactat tttagtttct ttaaaagcgg ataaatctaa
     1741 aggtgcagca gcacttaatg atttcccagg tgctaaaaat cttttggcaa aaattaacac
     1801 ttggcgtcag agtcttgaag aatataaaga aaggttagtt actacacatc cagagctaga
     1861 taaattaatc gtctctattc aagatttttg tgtgcaaaga gagggtagat atccagatta
     1921 caatgctaaa gttagcgcag ttgataacat catagatgga atgttatctt catttcagag
     1981 ccttggtggt aatgagtgtg aactccttcc gaaaaatgag gacatccccg tcccttttga
     2041 cggaggtaat ttagtttcct atttagaggc attagcccag gaaaatggca gtgagcaata
     2101 tgtcgaatat ttagtggcta ggatacgcac aatgcttgct gatacgagaa tgaaacctat
     2161 aaccaatgac tcagagcacc gagttgactt agccaattgg ttagaaactt atattggaaa
     2221 ggatggaacc ggcgatgatg atagctgtgt ttcaattatt gatctttctt tagtaccaac
     2281 agaaattact catcttgtga cagctgttat ttcaaggatt gtgtttgaat cattgcaacg
     2341 ttataggcgg ctatataata aatctttacc tacggtactt gtcgctgaag aagcacatac
     2401 atttattaaa cggtatcgtg atgactctga aaatcaggat gtcgcagccg tttgctgcca
     2461 agtgtttgag aaaatcgcac gagaaggcag aaagtttggt cttggtatgg tgatttcctc
     2521 acaacgacca tcagagttat cacctacggt tttgtctcaa tgtaatactt ttcttttgca
     2581 tcgaattagt aacgacaaag atcaggagca agttcataaa atggtacctg ataatttgcg
     2641 aggattactc cgtgaactac cttcattacc atcccaacat gcaatactga tgggctgggc
     2701 atctgaactt cctgtcctag ttaaaatgaa aaatttgacg aaagagcagc aacctcattc
     2761 tgatgaccct gacttctggg atgtttggac aagaaagtat gcggatggga agttggtcga
     2821 gagaactgtc gattgggaag ctgttgtaaa ggaatggcaa caaaagtgat ctgcagcaga
     2881 aaatgaaagc gggagagcag atcggaattg aaggaacttt atgccatcaa tctacttggt
     2941 gttgcgttcc attgtccctc cccccatgac gactgaagtg gctgcaaacg cagtacctaa
     3001 caatctcaat ataaacaatg aactaacctc ttagcgtggt tttttgcatc attggccctc
     3061 aaaccccgct aacagctcgc ctgttagcca tcgagggatt ggacaaatcc ggccacgccg
     3121 gatacgtcag caaatatcgc tgaagaatta gcttacacgc tcgtaagcgg ctcttacgac
     3181 ccgctaacgc ggagatacgc cccaactgcg gcttcgcctt gttgtgacca ctccgaccgc
     3241 gcacaaaagc ctctgagccg cttttagcgg aatatggctt tgcagggtga agggatggat
     3301 aggcgatccc tttcgatccc tcactggtat cggcggctaa ccctcggtgc tacctgtcat
     3361 ttcatcctgc cgccttacat gcgctaacgc gcataaatcc ccgttcctca gtggaacgaa
     3421 aactcacgct aacagggtag tgaccgggta gccacgataa ctgccaacaa aattagctta
     3481 taggttaatt ctaccttgtc tgaaattagc ctataggtta atattaatag aaatgggagg
     3541 tcacatgttt agcgttgttt atcacccgga agccagggaa gaagccacag cattacccgt
     3601 aaaaatcagg gtgaagtttg accgtctcat cggcaaactg gagtatgacg cccgattact
     3661 gcgggagccg gacacgaagc ccctgggcga tggacttttt gagatccgca cgatggggac
     3721 ggacatagcc aggggaatct gggtatacca caaaggcaat accatcatca tgctccgtgt
     3781 tttcatcaag aagtcacaga aaacgccagc gaaagaaatc gatctcgcca aaaagcggtt
     3841 agcggaggta cttaatgaaa ctggtaagcc ataaagaact gcataacgag tggatgcagg
     3901 acgatgaata tcgcgctgca tggcaggaag aagaacgtaa ggaaaagctc agggagctgc
     3961 ttgctgaatg gcgcaagcat gacaacctga caaaagcgca ggttgctgaa cgcatggggg
     4021 ttacacctcc ggttatttct cgcctggaaa acaacatcac caaagccagc atcgacacac
     4081 tgacccgcta cgctcacgcc tgcggcatca agaaacctgt cattgccctg tactgaatag
     4141 ccagccctcc ggggctggcc tttttgtttc ggtcagtccc aaaattttgt taaagcaagt
     4201 ttaaaatgtc acctgtttct ccaaagttga aatgtcactt ctaagccaat ctatttatac
     4261 ataatcttta catctttccc cggtgacaaa gtgctaattg gtctgtaatg ttcaatgtgt
     4321 ggcaggcaag ccgccggtga cttagggaag gtgcgaacaa gttcctgata tgagatcatc
     4381 atattcatcc ggagcgcatc ccagagggac atcatgagcc atcaactcac cttcgccgat
     4441 agtgaattca gcactaagcg ccgtcagacc cgaaaagaga ttttcctctc ccgcatggag
     4501 cagattctgc catggcagaa tatgaccgct gtcatcgagc cgttttatcc caaggcgggc
     4561 aatggccgac ggccctatcc gctggagacc atgctgcgta ttcactgcat gcagcattgg
     4621 tacaacctga gcgacggtgc catggaagat gccctgtacg aaatcgcctc catgcgcctg
     4681 tttgcccgat tatccctgga tagcgccctg ccggatcgca ccaccatcat gaatttccgc
     4741 cacctgctcg agcagcatca actggcccgt caattgttca agaccatcaa tcgctggctg
     4801 gccgaagcag gcgtcatgat gacccaaggc actttggtgg atgccaccat cattgaggca
     4861 cccagctcga ccaagaacaa tgagcagcaa cgcgatccgg aaatgcatca gaccaagaaa
     4921 ggcaatcagt ggcactttgg catgaaggcc cacattggtg tcgatgccaa gagtggcctg
     4981 acccacagcc tggtcaccac cgcggccaac gagcatgacc tcaatcagcc gggtaatctg
     5041 cttcatggag aggagcaatt tgtctcagcc gatgccggct accaaggagc gccacagcgc
     5101 gaggagctgg ccgaggtgga tgtggactgg ctgatcgccg agcgtcccgg cagggtaaaa
     5161 accttgaagc agcatccgcg caagaacaaa acggccatca acatcgaata catgaaagcc
     5221 agcatccgtg ccagggtgga gcacccgttt cgcatcatca agcggcagtt cggcttcgtg
     5281 aaagccagat acaaggggct gctgaaaaac gataaccaac tggcgatgtt attcaccctg
     5341 gccaacctgt ttcgggtgga ccaaatgata cgtcagtggg agagatctca gtaaaaaccg
     5401 gaaataacgc cagaaatggt ggaaaaaata gcctaaatag gctgattcga tgtgtttgcg
     5461 ggaaaaaaat cggcccagat ccgcgaaatg gctttgttga ataaatcaga tttcgggtaa
     5521 gtctcccccg tagcgggttg tgttttcagg caatacgcac gctttcaggc atacctgctt
     5581 tcgtcatttt gttcagcgct cgtaccaggg ccatagcctc cgcaacctga ccatcgtagt
     5641 cacgcagcgt cagtgaaccc ccgaacagct gttttacccg gtacatcgcc gtttccgcta
     5701 tcgagcgacg gttgtaatct gttgtccatt tccaccgcgc attactcccg gtcattcgct
     5761 gattagccac tgcacggtta cggtctgcat attcaccggg ccagtaaccc gcaccttttc
     5821 ggggagggat aagcgcgctg attttcttac gccgcagttc atcgtgacag agccgggtat
     5881 cgtaagcgcc atcggcggcg gctgacctga ttttccggtg ggtttgccgg attaacccgg
     5941 ggaaggcctc tgagtccgta acattgttca gcgacaggtc agcgcagatg atttcatgtg
     6001 ttttactgtc aacggcgaga tgcagcttac gccagatacg gcggcgttcc tggccatgct
     6061 ttttgacttt ccactcgcct tcaccgaaga ccttcagccc ggtggaatca attaccaggt
     6121 gtgcgatttc accccgggtg gacgttttga aactgacatt aaccgacttt gcccgcctgc
     6181 tgacacagct gtaatccggg cagcgtagcg gaacgttcat cagagaaaaa atggaatcaa
     6241 taaagccctg cgcagcgcgc agggtcagcc tgaatacgcg tttaatgacc agcacagtcg
     6301 tgatggcaag gtcagaatag cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat
     6361 acctggcctg aatagcttca tcatccagcc agaaagttat ggagccacgg ttgatgaggg
     6421 ctttattgta ggtgggccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg
     6481 cgttgtcggg aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac
     6541 aaagccgcag cgccatgggc acaggctcca gcaacttgaa gtaccatctg tataggtatg
     6601 actacagagg tctgaactct atacttgtga aggaacagta atatactgcc ctccgtcgat
     6661 ccagagtatg gggttaccgc atcagatttt actgagcaaa caatcacgaa aaagttactt
     6721 ttttctcagg aggtgcctgg acccttgccc ttcattttga cgcgagcagg atgtcagcac
     6781 cttacgtgcg gttactgtta ttcgcgtcct ggtggtatag tgcagcggct ggcagaagta
     6841 acctactatc caacggaata agatcacgat gaacgtcatt cagccccgca tgctctctgt
     6901 atttgacttt gacggtacgt taacgcggca tgatagcttt atcccttttc tgcggtttgc
     6961 ctttggcaat cgggcgtttt cgcgacgtat cgtcagcatg gctctaccca cgttacgctg
     7021 tatgggtaag cagatgacgc gtgatgagct aaaagaaacg ctcattacaa cttttcttac
     7081 tggtgttgag gaaaagtggg tgtcttctaa agcagaggaa ttctgtcagc tctactggca
     7141 tcgcctcatg cgcccggcag gtttgattgc tgtggcgaat gaagtcactt ccggcgcgat
     7201 cgtcacctta tgttctgcgt cgccggaaat tgtactcaga ccctttgccg aaaaacttag
     7261 gatcaaactg attggtacac aattagagac aaaagacggc attctgaccg gcagaatcag
     7321 tggtcataat tgcaggtgct cacagaaaat tatccggctt gaaaaagaat atggacctct
     7381 gactgactat catcttcggg catggggtga tacgcggggt gattatgaac tcctttccgc
     7441 agcgactgag cctcactggc gtcacttcca tcaaggtttc cgtcgtaaac ttccgtcgaa
     7501 gctaatcatc agataaaaat tgccctttat cgttctgcaa tttcttcttt acataaaggc
     7561 aacatatatt ctacatttaa caaatgtaac tggtcaaaaa aagagtgcat cctggtaaat
     7621 cggtagctcg gactattagg tatgcttcaa cctgttacca ttggaatacc tactttcagc
     7681 ccgcttttcc gagcgggttt ttttcatccc tgccgatgtg ttcctgggtc taaattactg
     7741 ggtgaagttg ggtttcagcg atcacatctg cgtatagccc tgtttagctc tgcgtattat
     7801 gaaagggtat cgtcatggca gcaggcctat cgccccttac cggggcgatg attcagataa
     7861 tgccttcctt ttttgccagt ccaagccagt ctttgaactc ttcgagaagt ttttcataca
     7921 actcttcgtc ctttatgtgg gcaaaattat cgatcgcaaa aaagttgctg ttgtctaccc
     7981 gacggtcgct aaaggtgtcc agcctctcca gaatcccata atttgatccg cccagtccaa
     8041 cgaacttcca gaagaccgga tagttggctg agcggcggat ggcctcttta atttctcgtg
     8101 ttttgctgat cccgccatct gtaatgcaca caacaaaaac aggtaaggtg ctgtccttga
     8161 aataatcgat aacctcattc atgactggcg gctcattgtt cgtcccgcca aggccgggaa
     8221 ggatttccca ggcagatcgt ttacctgtgt tctgaatggc agcaatgtat ccgtcaagat
     8281 tgtccagtgt gacgtcagga tatttcttgt gacgctcagc aaagccccat acatccatcg
     8341 tgccgtcatc gtcgaactgg acagacagga cagctatacg gtcaagaacg gcctgtacat
     8401 gccctttttt aaactggccg gtcatagagc ctgaggcatc gagaacaaaa gcgactcgtg
     8461 cttcaacggt atccagtttg tgttttgcca gcgaaatact ggctttctta gccaggctga
     8521 taagcgcagg cgctttacct tcgagtttct tttcaaggct gatcctggct ggttgcggta
     8581 caacttctgc tgcaggttcc acgtccacac catagctaac tgccagtggc tcaagcccgc
     8641 cattaaatcc ctgcccaagc gccctgagct tccagttgcc ctggtatcgg taaacttccc
     8701 ccataattac tgccttttct gtgcggccat tgagatcgac aaaaaaggtg gcctgacttt
     8761 cagcagaaag tttcagttgc gcgaggccgc ttatcgtatc actgccatct atcaccatag
     8821 tgattgcaat cttctgaatc gtatccggga tctggtcaag cgcaaaatga acgactgcgt
     8881 gctggtgacc ggggttgagt ttcacactgc cacccgcagc actgagattg ttgtaaaaga
     8941 taaaatcggc atcgcctgtc acttttccct cagcattcag cataaacagg caagtctcag
     9001 gggtactgtg aaatccagga ctggggggat aatcaagccg gagagtcagt gtatctgtgt
     9061 gaagcggaat gttctgtcct gattgtaatt gcatttaggc tccttgttgc cgggtaaagg
     9121 gatcactcag cgggtagaaa aaaccccgga caggccgggg tttggtttta ctggtttatc
     9181 agatgttgat gccgtgctgg gttgccagcg cagccaggcc accagcaaag ccctgtccta
     9241 cagccttaaa cttccattcc gcgccatggc gatacagttc accgaaaatc attgcggttt
     9301 cagttgaggc atcttctgac aggtcaaagc gtgcaatctc ggcgccatta tcgttgttca
     9361 ccacacgcat aaagctgtta ctgaccatgc cgaaattttg cttacggctg tctgcttcat
     9421 gaatggtgac tgcgaatacg agcttcttaa cgtctgcggc cacttttgtc agatcaattt
     9481 tgacctgctc atcgtcaccg tcgccttcac cggtacggtt gtcaccctga tgctgtactg
     9541 cgccatccgg gctggtggtg ttgttataaa atacgaaatg cgaatcggac agcactttac
     9601 cgttttcgcc aacaaggaat actgatgcat ccagatcaaa tgcctgacca tcagtaacgc
     9661 gtgcatccca gcccaggcca accagagcaa cattcatgct cggggcttct ttagtcagag
     9721 aaacattacc gcctttaacg agagaaacag ccattttttg ctcctgctaa tcgtagatat
     9781 cgggtcgtgg atatcggggg catcaccccc gaatgatttg ggtaagaaag ttacttttta
     9841 tcaggacgcg ttgatgccgt actgagcgca cacggaagcc agtccaccag cataaccctg
     9901 gccaaccgca cggaatttcc attctccgtt gtgacgatac agctccccaa acagcatggc
     9961 agtttcagta gaagcgtctt ctgtcagatc gtagcgagca acttcagtct gattgtcatc
    10021 attcaccaga cggataaatg cgccggaaac ctgaccgaag ctctggcgac gagcctgagc
    10081 atcatggata gtgacaacga aaataatttt gtcgacatcc ccaggtaccg cgtccagttt
    10141 gattttgagg gattcatcgt cgccatcacc ttcaccggta cggttatcac cggtatgagt
    10201 aacggaacca tcagcagatt tcaggttgtt atagaaaatg aaatctgcat cgccacgaac
    10261 cttgccattg gctgccagca gaaatgcgga agcatcgagg tcaaagtcct gaccgtccgt
    10321 agagcgtgca tcccagccaa ggccaaccag gacatttttc atgcttggcg cggttttgct
    10381 cagggaaacg ttgccgcctt tagagagtga aacgctcatc tatttatcct ctttcgttaa
    10441 tcattaatca gctgagatca gctttcttta ttttcatctt ccggcttttc cggaaagatg
    10501 acactggcca gaataccaag agccagtaca cccagtacga catacaggct ggtggtcgca
    10561 gacaggctgt aaccatggtg ccagatgtga tcggtagcgt tcagacccag cttaaatgca
    10621 atgaagaaca gcaggacgat aacagctttc tccagctggg acagatactg tttcagggct
    10681 tccagtacga aatacagagt acgcaggccc agtattgcga acatcatggc gctatacaca
    10741 atcagtggtt cacgactgac tgcaataatt gccgggacag agtcgaaggc aaacatcacg
    10801 tcagagagtt ccactacagc cacacataac atcaatgggg tggcgtaaag cgctgccttt
    10861 ttcaggcgcc ctacagatac gtctttgttt tctggtttag ccagttcggc atccacttct
    10921 ttttgtgtca gcaggaatgc atgtccggac agtttcggcc aaatcgggaa gaagcgttta
    10981 accaggcgat aagcaaggtg ctgagaataa tcctcaattt cgtcatcatc cccaccgcct
    11041 ttaagcatca tgaccgcagt ccaggcaaca acaacggcaa agatcagctc gacataaggg
    11101 ccaaggctaa gcagaccggt accgatcgca acgaaaatcc cccggaatac gatagcgcca
    11161 atgatccccc agtacagaac ccggtgacgg taacggtccg gaactgagaa ccatgagaaa
    11221 atcgccatca tgacaaacag gttatcaact gacagcacct tctcaagggc ataacccgtg
    11281 acaaacaggc tggcaacctc ggctccgtga tgcacaaaaa ggaaaccggc aaatgccatc
    11341 gcgacaacaa cccagaaaac ggaccataag gctgcgcttt tgagggaaat tggcttgtca
    11401 tgtcggtgca tgaacaggtc aataaagatg gcgccgaccg acagcgcaac aaagaccgtc
    11461 actgtttcag ccggaaaacc gatgtgagtg gaaaccatat cgtaccttta tccgttgttt
    11521 atcagaggcc aaattcagcc gggtcgaaac ccagcgaacg ggcaatttca cggacagcac
    11581 tcttctcatc atcatcgaag ttgccatcac ttttagcgac agcaataccg acacgaagag
    11641 ccagttgagc tgcctctggc tggtctttca gggcgaggat atatttcatc gtttcgcctt
    11701 ttccgatttc cagatcaaag tcgaaactgg tcaccagctt gttaaaaaat tcgataactt
    11761 ctgctgtatc gaagactttc agctcttctg aggatcgcag aaatccaatc atcttctgtt
    11821 tttcttcgga gctgacaccg tcactggcaa cagcgatacg ggcgcatacc gcgacggtac
    11881 cctgcataaa tttcttgttt ttgtaacggc ctacctggcg ggtcaattcc tcacgacccg
    11941 aggtcaaagc ccctttgact ttgtcgaaaa aactcattct cataccctca tcattgcgtt
    12001 tatttggaac ctctggacca gcgaaacccc cagccaaaag cgcggtccat ttcctcctgg
    12061 cctctgaagt actggttaat acgttccaca cgaatggaac cgttctcgtt gaccagacgg
    12121 gcaatcgcac acagcgtgcg tttgttatca ccttcagtca gacgcgattc aatcggtggt
    12181 tgctcaggaa catgtaatgt caccacgcca tcggttttgt cccagctggg cacgccctga
    12241 tagataaagg cataaatcag tacctggcgg atttctttcc attcgcggcc attgatgtgt
    12301 agccattccc catccagagc ctcgccggta cgatcatcac cctgcagttg aacatatggc
    12361 tcagagtgat agttaccaaa agcattaccg agtgcctgaa caactgactt atagccatct
    12421 gcgagctcaa cgaatgcacc gagatccagg tctatcgctt tgttcgaacc aaacataccg
    12481 cccagtaacc cgcctgaacc cgagcctcga tgccagttca gattgatgcg gatttcacca
    12541 aaattgtctt ttttggcaag gctgatggca ggcttctctt tggttagaga aactttactc
    12601 aggctaactt taggcggagc cggaggcgct ggtggcggag tgactggagc tggctcatcc
    12661 gcgacgttga cgccatagta ctcagcgaga ggtttcaggc cgccattgaa gccctgggca
    12721 ataaaccgaa atttccagtc accattcctg cgatagactt caccgaggat cagagcggct
    12781 tcctgacggc cgctcagttc tacctgacca ctgatcagcg aggtattgcc actttcaatt
    12841 tgtatggaca gatgattcag ggaggaaact gtatgactgc cgtcacaggt aacggtgaac
    12901 gcgactttct gcacgtcggg cttgaggcgg gacagatcaa cggtgaaaac gctattggtc
    12961 ccttcagtac tgaagctaat ggaaccatct tcattacggg gttgcccgta aaagaccata
    13021 tcgctgtcac cccgaacttt cccgtcagcg aataatcgaa atgccgaggc atcaacagga
    13081 ccaccagaaa tgactctaac gcgcagttcc tgagcaggta cgggggcatt gccccccggt
    13141 gtcagattca tgcttttacc accgtactga tgatatcgcg ctgcatatcg tcgattgtgc
    13201 ggccaccgct tgctttaccg tgggcggtaa agtcccagtg gccgttgttg cggcgcaggg
    13261 aagcgataat gatgccggta tgtgggcctt gttctgtcag ctgatagcgg gcaagttcct
    13321 tattggtctg atccacgacg cggcagaagg cgttatccac gtcgttgaag gactgtccac
    13381 ggaagctgtt cacagtaaaa gcgaggtatt caacatttgc cggcaatttt aagaggttga
    13441 cgtgaatgat ttcatcatcg ccatccccct cacccgtcag gttatcgccg gagtggacga
    13501 cagaaccaca gctggatttc agtttacgga accagatggt gtcaatttta ttacctgcca
    13561 catccagaag aacgcaacct gcatccagat ctatggaatc attgccacca aaaaagctgc
    13621 caaataatcc ctttttctta acaggatccc aacctaatcc gaactgaagc tggctgagat
    13681 cggtggattg tttgctgagt gataccgtct ggtttttact taaagaaacc atattcattc
    13741 cttaaaggta ttgactgagg ttttttaaaa ttcatattca gtgaagcaac ataaaaatca
    13801 tatcatgaca aagtttactc gcaagattaa aactggtagg ttttcataat ttacgtttca
    13861 ttaca
//
LOCUS       DAFNBJ010000127        13785 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000127 DAFNBJ010000000
VERSION     DAFNBJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.127"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            163..381
                     /locus_tag="L2X49_003602"
     CDS             163..381
                     /locus_tag="L2X49_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBN4798623.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            599..841
                     /locus_tag="L2X49_003603"
     CDS             599..841
                     /locus_tag="L2X49_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798624.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1171..2100)
                     /gene="fdhE"
                     /locus_tag="L2X49_003604"
     CDS             complement(1171..2100)
                     /gene="fdhE"
                     /locus_tag="L2X49_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBN4798625.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2097..2732)
                     /gene="fdoI"
                     /locus_tag="L2X49_003605"
     CDS             complement(2097..2732)
                     /gene="fdoI"
                     /locus_tag="L2X49_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBN4798626.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2729..3631)
                     /gene="fdoH"
                     /locus_tag="L2X49_003606"
     CDS             complement(2729..3631)
                     /gene="fdoH"
                     /locus_tag="L2X49_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBN4798627.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNVGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3644..6694)
                     /gene="fdnG"
                     /locus_tag="L2X49_003607"
     CDS             complement(3644..6694)
                     /gene="fdnG"
                     /locus_tag="L2X49_003607"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6107..6109),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBN4798628.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6888..7721
                     /gene="fdhD"
                     /locus_tag="L2X49_003608"
     CDS             6888..7721
                     /gene="fdhD"
                     /locus_tag="L2X49_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBN4798629.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7874..8914
                     /locus_tag="L2X49_003609"
     CDS             7874..8914
                     /locus_tag="L2X49_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HBN4798630.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRVRDKVPYSDWDKEQLQDANSSWMVDDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(9005..10711)
                     /locus_tag="L2X49_003610"
     CDS             complement(9005..10711)
                     /locus_tag="L2X49_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HBN4798631.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGYNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAHPIEVNNEIINHVLIACAAADARHELKYLAIWQAYCVSIRQRLL
                     PG"
     gene            complement(10711..11781)
                     /locus_tag="L2X49_003611"
     CDS             complement(10711..11781)
                     /locus_tag="L2X49_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBN4798632.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGDKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            complement(11771..13222)
                     /locus_tag="L2X49_003612"
     CDS             complement(11771..13222)
                     /locus_tag="L2X49_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HBN4798633.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTLVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(13233..13679)
                     /locus_tag="L2X49_003613"
     CDS             complement(13233..13679)
                     /locus_tag="L2X49_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBN4798634.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
BASE COUNT         3353 a         3536 c         3589 g         3307 t
ORIGIN      
        1 aaccattttt gagtaataaa tgaaacaact gcaacactct tattatttct ggaaaacgcc
       61 tcgcaaaaac aaaacaacat gcaaaaatcc gcttttttta aatgattttc cggcgtaatt
      121 ttcgcacata cattaatgag taatataggt aaggtggtgg taatggcgat gaatacggtt
      181 tttcttcatt tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag
      241 gtttcacgat ctgcaatttt acgcgaagcg gtagagcaat atctggaacg tcagcaattc
      301 ccggtgcgta aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag
      361 tgtaaggagc ataaggaatg acaaaaattt ttcattcttg aatataaaaa acagatgccc
      421 ttattctggt attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca
      481 tcatatctgg aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa
      541 gattcggcaa gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat
      601 gaactctctg gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat
      661 taagcaactt gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga
      721 agcggtagat caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat
      781 ctggcaaggg tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta
      841 atgggaaaaa atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc
      901 ggcgtgaacg ccttatccgg cctacaaagt cgtataaatt caatgtattg cagtttattg
      961 gtaggcctga taagcgcagc gcatcaggca atcttacgct tgtcatcagt ctcatgccca
     1021 taaccttatt gatgtggtgg tgggtaaatt gaggccaaac cggcactggc ggcatagctt
     1081 gaggtggtaa cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg
     1141 cgacctgcgc cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg
     1201 gttgatggaa ctgcgggcat agccttcttg ctccattcgt gcgtccagta ccagagaggc
     1261 gaggtcatct gctacggctt cgatttttgg atctttttcc tgatagagaa ttttcaggta
     1321 agtgccgcag tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca
     1381 gtaatgcagt ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc
     1441 agtttcacac aggttgcagt gcaggtaacg tagcccctga gtggtgccaa tttgcaccat
     1501 gctggacacc ggcatagaac cacaaaccgg gcaatattga cgttgctcgc cgtattcagc
     1561 gcgggctttg ccggggatca gattggccat ctgcgcccag tagagcgaca gtgcagccca
     1621 gataaacggc gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc
     1681 catatcttcc agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg
     1741 gccgctcatt tcaggtttca gctcagcaat cagcgccatc agcagctttt gccagtgctt
     1801 atcacgcggc agaacgtgaa tatccagcgg gggcttgcct tgtgcgctgg cttctttaat
     1861 gcgcgcagtc agatccatct ccagcggatg gtcgtacagc accacttcct gggcgtgggc
     1921 gataagcgca gcaaagcgca ggtaatcacc cagaggatta ttttctgcca gctcgcgcag
     1981 acgctcggcg cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat
     2041 atccgccgta cgtttctcgc ttgaacccag ctcatcttgc gggattatgc gaatactcat
     2101 tcagctttct tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc
     2161 gcccaggcgc tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg
     2221 gcggcgtaga tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc
     2281 attaacgcga atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata
     2341 atcacgccgc tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc
     2401 tgaccgaagt tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata
     2461 ttcttcgccc aaaagatatc atcccgattg attaggttgt gatgccagta acggaaaaac
     2521 atgatgatga acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc
     2581 tgcggtgtgc ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg
     2641 ctcaccgccg ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg
     2701 gtgtagcgca cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca
     2761 gattattctc ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg
     2821 ccgcaaaggt agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt
     2881 tcacggtttc gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag
     2941 catggtgcag cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg
     3001 cattgtcgta accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg
     3061 actctttcgt accgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt
     3121 gcccaaccac cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt
     3181 tgaggcgcgg aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct
     3241 cggactggaa gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg
     3301 ctttcaggca gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca
     3361 gtttgtcgtt ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat
     3421 cattggggtt gtcgtacacc ccaacgttat tgccgacggt atcgcgaatg tcgttccact
     3481 ctgaacacgc cacctgacag gctttacagc cgatacaggt ggtgacgtcg atgagtttcg
     3541 ccacttcttc ctggaagtcc cgcgcttgag gcgcgggggt cagaccgtta gtcgcggaac
     3601 gacgaatgat atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat
     3661 tcacaaggaa ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta
     3721 acgtattggc aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac
     3781 cgatggtatc gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg
     3841 ctttggcttt gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc
     3901 caagtttatt cgccagcgac tcaccgattt ccacaaactg ctctggctgc aaaatcgcgt
     3961 tcaacagcgc gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga
     4021 catacgggaa cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag
     4081 ccgggttcga gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct
     4141 cgtagtgttc cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc
     4201 cttcctgctg catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg
     4261 gaatatccca gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc
     4321 acgggttacc ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc
     4381 atgcccatgc ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg
     4441 ccatctggtt gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg
     4501 taccgtcatc gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg
     4561 caccggttgc cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt
     4621 cttccgactt cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg
     4681 ggttcgcgcc accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga
     4741 tctcaccgtc agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc
     4801 agcgacctga gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga
     4861 acacttcggt ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga
     4921 agttagaggt ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac
     4981 aaccgatcac tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat
     5041 agccattgac cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat
     5101 cccacttcgg caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga
     5161 aggccttcat catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt
     5221 ccagcagcgg ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc
     5281 ttggcagcgt catgtaacct ggcagactct gcgacagcag ccccaggtcc gtcagcccct
     5341 gaatattgga gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga
     5401 gcagcagctg gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc
     5461 agccgagggc atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt
     5521 cgcagacttt caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg
     5581 tgtagcggga aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg
     5641 tatcgcgttt ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact
     5701 tgcgtttttc cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca
     5761 ctatcaggct tgcgttggta taggcttcgg tgtattcgcg gttgaatttt tcattgttca
     5821 gcaggtacag caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat
     5881 agtagtcagc taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc
     5941 cgttgtgaat tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt
     6001 taccgcccat caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg
     6061 caccgcgacc aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct
     6121 ggttgtcgac cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt
     6181 cgttactcga cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc
     6241 cttcggcgtt ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc
     6301 gatcaaacgc ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt
     6361 ccggaaactt cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac
     6421 aaagtgcacc gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt
     6481 tggcgttttt tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat
     6541 aggtgcaggt attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga
     6601 gcgctacgct gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc
     6661 agatcttaaa gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc
     6721 acgtcccatt tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg
     6781 aaggggccgc aaaggtccag aattggctca atttccctcc atccaggaag ggtttgtaac
     6841 agaataccat aatgttggtg tgtgtgttct tatccggtta agagaaagtg aaaaaaacac
     6901 agcgaaaaga aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc
     6961 ggcgtgatga tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc
     7021 tggtctacaa cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact
     7081 ttgcgctcgg tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca
     7141 tggatgtcgt tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct
     7201 ttatggggtt gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg
     7261 gcgtggagca acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt
     7321 ttgatctcaa caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc
     7381 aactgactgg ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg
     7441 ggcatgaaga cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag
     7501 aaggggaaag ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg
     7561 tgcaaaagtc ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc
     7621 ttgctgtaga agtggctgag cgttgtaatc tgacgctggt gggtttttgt aagccgggtc
     7681 gggcaacggt ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat
     7741 atatattaaa ttgcaggact tattcatttc gtgaatttta ttattttatt tataaataaa
     7801 acgcgtgtag tggcgttttc ttttttcatt tattatgttc actctcttat ctatatattt
     7861 ataaggcaat taaatgaaaa ggaatttatt atcttccgct attatagtcg ccatcatggc
     7921 cctcggtctg acgggttgtg atgataaaaa agccgaaaca gaaacgcccc cgcctgccaa
     7981 tagtcaacct gccgcaccag ctcctgaagc tcccgttgca aaagcagaag ctaaacctga
     8041 aacacctgcg caaccggtgg tcgatgaaca agcggttttc gacgaaaaaa tggatgtcta
     8101 tatcaagtgc tataacaagt tacagatccc tgtacagagc agtctggcgc gttatgctga
     8161 ctggctgaaa gattttaaac aggggcctac cggtgaagag cgtactgttt atggcatcta
     8221 cggcattagt gaatccaacc tcgctgagtg tgaaaaaggc gtaaaaagtg ctgtggcgtt
     8281 aacgcctgcg ctgcaaccta ttgatggcgt ggcggtgagt tatatcgatg ctgccgtggc
     8341 gttgggtaat accattaacg aaatggataa atattacacc caggaaaatt ataaagacga
     8401 tgcgtttgcg aaagggaaaa cgctgcacca gacattctta aaaaatctgg aagcctttga
     8461 agctgtagca gaatcttatc atgcggcgat tcaggaaatt aacgataagc gccagcttgc
     8521 ggaactgaaa aatattgaag aaagagaagg gaaaacattc cactattact ctctggcagt
     8581 catgatttca gcgaaacaaa ttaataacct gatatcgcaa gataagtttg atgcagaagc
     8641 ggcaatgaag aaagtgtctg aactggaaac gctggtggcg caggccaaag aagcggataa
     8701 aggcggcatg aatttctcgt ttattaattc ggcaggccag tatcaactcg aggctaaaaa
     8761 atacgttcgc cgcgtcagag ataaagtccc gtactctgac tgggataaag agcaactgca
     8821 ggacgcaaac tcaagctgga tggtcgacga ctctttcccg agagcattac gcgagtacaa
     8881 cgaaatggtt gatgactata atagcctgcg ttaaacgttt ttgatgataa atgcagtgtc
     8941 ggatgcggcg catccgacac agttcacccc ttgtgaagta actccataaa tgcctcatat
     9001 cctgttaacc cggcaataac ctctgccgga tgctgacaca atacgcttgc cagatagcta
     9061 aatactttag ctcatgacgc gcatcggcgg cggcgcaggc aatcaacaca tggttaatga
     9121 tttcgttatt cacctcgatc gggtgggcga gggtaataaa aaatccacga aaacgtcgct
     9181 cctgttcgct ccagcaatgt gggatggcga ggcgattaac aatcaggttt tcaccttcgc
     9241 cttcgcgggc gatgatgcgt tgtgcttcat cggcggtaat atggtgttgt gccactaatt
     9301 gcgcacaaat ttgccgggta atatgttgcc agctttcacc gcgtacattc gaataatgaa
     9361 aacttccggg ggcagagaaa aaacgctccg gttgctggcg aataaatgcc gtcgccagaa
     9421 aatgttttat ttgttcgata ccggcagccg taatgatatt ttttacggtg atgtaattat
     9481 ttaccgcgtc ttccagtaaa taatggctat tgttaatgat caataacggc tcaatctctt
     9541 cgcgaatggc aacaagttcg cttaagctac gggcaataat tacccgacaa tttaaaacat
     9601 cgcgctcaat ggcgagctga ttaatagtgg caatcgcgtt ctggtccgag agcaaaatga
     9661 tcggctggcg ttcgttttga tgccgctcca gcgcacaggc aaaatacaaa ccgatcagat
     9721 cgctgtcgaa aaggggaata tcgagctgtt cacgcagtag cgtaataaag tgcagactca
     9781 tatcaaaggc cgcaggccag gcggctttca ggttattcat gctgctttgg cggtgctcag
     9841 caatccacac tggtgcggcg aggcaacgta ataaatgccc ggtaatgttc tcaaccagct
     9901 gcgtatcaat aatattcaat gacgcgatgc ccggcaccct ttgcagcaac cccagcacat
     9961 gatcggctga aattacctgc gcttgctgat gctgtttctc tatcaaaccc gtcagggttc
    10021 ttacagcgtt ataaccaaga aacagaccgc tatgttcaac gatttgtttt atctcgtcac
    10081 cgggatattg cggccactca tcggtcagtt gattacgcag ggcgtaaatc gccagaatca
    10141 ggctggagag ataatcaccc tgcatgagcg gccagcggtg ttggttgtcg caggccgtgg
    10201 ataaatgctg aaggttgtct cgtgttatgc ccgccagcgg aattaaaaac ggatctttgc
    10261 gcagcaagtt cgccagcaag ataacgcgtt tctcttctgt ctcatcaatg aaatggccca
    10321 aaccagggcg gctggccagg caacaagtgc gttcataacg ctgttttaac cggggcagac
    10381 gctctgctac ccacgtttct ggcaaattaa gcgccgaggc gagttgcgca cggggagtga
    10441 aagtattcag taataacagc gccagcagcc gatcgtcgtt atcgtgcttt tgcagcaact
    10501 gaaaaaagct gcggcgctcg aagatttcca gctgatagcc cgcactgcca ctggcgaaaa
    10561 tgcgggcttt gccgttaagg gtgaagttga gatagtcaat atcacgcagg atggtcctgc
    10621 ctgaaacgcc agtctgttgc gccagctcgc caggcgtgcg cggctgttgc tccagcagat
    10681 cgacaatctt taactggcgt tcgtttagca ttaatccacc tgacggaact ggctaaacgc
    10741 gttgactttc tccgcagtta aggtcgtcag aaaaccccgt atcagcgtga gcagagcatc
    10801 gtaatcggct tttgaaatca taccgctgtt ggcgtgcaga taacgagttg gcagacacag
    10861 cgcgacaacc ggacgcccgc cgcccatcac gttgtagcgc ccgccatccg tcgctccggt
    10921 tttcatggtg gaaaattgca gcggtaaatc attctgcgtg gcacagtttt taaacgctgc
    10981 taccagtttc tggttgggga agtagcgctt gtcaaacagc atcagccccg gcccctggcc
    11041 cagtttcagc gggtatttaa tgttatcaat gcccggaaca tcacctgcga cagcggtatc
    11101 cagcacaatc accacatccg gtttaatgtg ttccgccgag gtttgtgccc cgcgtagccc
    11161 aacttcttct tccacgctgc cgacgccata cagcgtaatt tcgggattat tcaccgtctg
    11221 caatagttca gccatcattg cgcagccgat gcggttatcc agcgccttgc cgaccacttt
    11281 atcttcgccc cagcaggcaa aattggcttc cgggctaata aaatcgccca tcgccacacc
    11341 gcgtttttcc acctcatcgc gactgttcgc gccaatatca atgaacatct catcaaatga
    11401 cagcggctgt tgcctttgct tttccgttaa cgcatgaggc gcgacggaac caatcacacc
    11461 agggattttc actcccttgt gtgtgcgtat ggttacccgg tggttgagca tcgactgatt
    11521 ccaccagcca ccaatggtgg tgaaacgcag aaaaccgctc tcgtcgatgt gggtgaccat
    11581 aaagccgact tcatccatat gcccgacaac ggcaactttt ggacctttat cccccttacg
    11641 ggcaacaaag ctgcccagac catcaaaggt gatctcatta acgcaaggtt ccagcgtgtt
    11701 tatcagaatg tcgcgaactt cctgttcatc gccgctgacg gcgctggctt cgcacaactg
    11761 ttgcagtaac tcaatgttca tggacaagct cctgttgcgc ggctttcgct ttgcggcgta
    11821 accacaatcc tttcaggacg ataatgaatg aaatgttcag cgcgaggcca atcgccagca
    11881 ccagataaaa cgaacccacc ggcgacatca ggccgatcag cgggtcaaag atacccaaac
    11941 caggtgccag acgtttgatt ccgaaagcga tcaccagcac gccggtaatg ccacccgcca
    12001 gcgtattggc ggtaatcatc ggcaacggag ctgccagagc gtaaggtata gccggttcgg
    12061 ttgcgactgt ggcaccgacc acaatggcac tactggcagc ctctttttcc tgtcgggtaa
    12121 acagtttggg cgcaatgaac gtcgcaagac ccgccgcaac gggcggcatc aacgcgacaa
    12181 cgccgacgat ggcgtaccag tcataaatat gtttttccag cagtgagaag cagaagaacc
    12241 aggcggtttt attgatgggg ccgcccatat cgaacgccag catcgcaccg accagaaacg
    12301 ctgccgcaaa cttcattgat ggaggaatgg tattcaggaa gtggagcagc ccgcccatga
    12361 ggtcggacat caccggcccg ataacgtagt aagtgagcac gccgaaaacc agcagagtaa
    12421 cgaacgggat cagcattgaa ccgagcagcg gttgtaatgc tttgccaagg cgcactttgc
    12481 ggaaccagaa gacgaaatag ccaatcgcca gccccagcac caccgcgccg aggaatcccg
    12541 cacccgactg ggtgccgagc agcgctttgt cgttcgccag atagcaaacc agaaacgccg
    12601 gagcgaaagc cggtttatcg gcaatcgacg aggcaatata agcgcccatg atggggatca
    12661 taaaggtgaa gccaaggtaa ccgatggatt caactaccaa ggtaaacgac ggcgcgcctt
    12721 tcgacatatc ggtatacggc aaaccgaact gcaccagcat gttggcaagc gccaccagga
    12781 taccaccgcc gatgacaaac ggcagcgccg cagagacgcc cgccatcagg tggctcatca
    12841 cgctgccgct ttgcacttcc tgtttgccaa gcttcacgcc gctatctgcg gcaaaaagct
    12901 gcgagtttgt cggtaattcg ctgaaaatct ggtcgatatt tttcaacgcc tgggagatgg
    12961 caatctcata aactttcttc ccggcaaacc gcgcgcgatc atcaccgctc aggccacgcc
    13021 cggtagcgag aatgacgtaa tcggcagcgg caatctcttc gctggataag cggttttcaa
    13081 cgccactgga cccttgagtt tccactttta tggtatggcc gagagaacgg gctttctgtt
    13141 ccagggcttc cgccaccatg taggtgtgag cgatcccggc ggggcagttg gtgatcgcga
    13201 caatacgtaa ggatgactcc atcgccactt ccttatgagc tgagggtttg atttaacaag
    13261 gcaagcacct ggtcggcgtc gccctgttgc agttgatgaa taaaatcctg gtggataatt
    13321 ttgcgacaca gtgtgccgat gattttgacc tgatcctctt cgccgctttg cggcacgccg
    13381 aggcagatcc agcaattgac gtcttcgcca tcgctggctt tccagtcaat agcctgcgct
    13441 ttgcgggcga ataatacgaa cggttgttta acgcaggcgc ttttaccgtg cggcacggcg
    13501 acaccggaac caaatccagt agagtgcatt ttttcgcgca gcagcagggt ttgcaggaac
    13561 tggtgtgagt cggtgataaa accgttttgt aacgctattg tcgccaactg cttcagaacg
    13621 gaataagcgc cattgccctg aatattcagg tcaatacagc ttgcagtaag agctgccatt
    13681 aatcggactc cataaaatat attttaatca attgtaatgg tggagcgatt aagtgatgag
    13741 aggcgatatg tcggtaaaca atgacaattt gagacagagt gaaag
//
LOCUS       DAFNBJ010000128        13765 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000128 DAFNBJ010000000
VERSION     DAFNBJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.128"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..755)
                     /gene="yhaJ"
                     /locus_tag="L2X49_003614"
     CDS             complement(<1..755)
                     /gene="yhaJ"
                     /locus_tag="L2X49_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBN4798635.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEK"
     gene            860..1561
                     /locus_tag="L2X49_003615"
     CDS             860..1561
                     /locus_tag="L2X49_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBN4798636.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1584..1748
                     /locus_tag="L2X49_003616"
     CDS             1584..1748
                     /locus_tag="L2X49_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798637.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1960..3270)
                     /locus_tag="L2X49_003617"
     CDS             complement(1960..3270)
                     /locus_tag="L2X49_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBN4798638.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3298..4629)
                     /locus_tag="L2X49_003618"
     CDS             complement(3298..4629)
                     /locus_tag="L2X49_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBN4798639.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4904..6268)
                     /gene="tdcG"
                     /locus_tag="L2X49_003619"
     CDS             complement(4904..6268)
                     /gene="tdcG"
                     /locus_tag="L2X49_003619"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBN4798640.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6340..6729)
                     /locus_tag="L2X49_003620"
     CDS             complement(6340..6729)
                     /locus_tag="L2X49_003620"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBN4798641.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSYVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6743..9037)
                     /gene="tdcE"
                     /locus_tag="L2X49_003621"
     CDS             complement(6743..9037)
                     /gene="tdcE"
                     /locus_tag="L2X49_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBN4798642.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9071..10279)
                     /gene="tdcD"
                     /locus_tag="L2X49_003622"
     CDS             complement(9071..10279)
                     /gene="tdcD"
                     /locus_tag="L2X49_003622"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBN4798643.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10305..11636)
                     /gene="tdcC"
                     /locus_tag="L2X49_003623"
     CDS             complement(10305..11636)
                     /gene="tdcC"
                     /locus_tag="L2X49_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBN4798644.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11658..12647)
                     /gene="tdcB"
                     /locus_tag="L2X49_003624"
     CDS             complement(11658..12647)
                     /gene="tdcB"
                     /locus_tag="L2X49_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBN4798645.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12746..13684)
                     /gene="tdcA"
                     /locus_tag="L2X49_003625"
     CDS             complement(12746..13684)
                     /gene="tdcA"
                     /locus_tag="L2X49_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBN4798646.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3573 a         3724 c         3241 g         3227 t
ORIGIN      
        1 tctttttcga ccatcggata cggcatcgtc gccacgccaa gccccgccag taatgcctga
       61 cgtttatctt caatcgtgct caccgttaag cgcggctgtt tgtccagcag ctgtacggtc
      121 aacaccgggc gctcacgagc ggtatccgct accgcaattc cacgatattt cacgcgcgtc
      181 acttcagata acggttccgg ctcctgatga atcgggtgat caggcgcggc aacgtagacg
      241 ttcattaacg tatagagctt gcgcgagttg atctccgacg aggaacgaaa atgcatatcc
      301 ggcgcgataa caatatccgc ccgcccctgc tccagtcgtt cccacgcccc cgccagcact
      361 tcggtgatga ttgccagttg ggtattggct tttgccgcca gtttgtcgat taacgggaaa
      421 aaggcaggtg tcggtaccag cgcttcggtc acaatggtga gatgcgtttc ccaaccgcgc
      481 gcgagggctt ccgcatcggt agtcagttta tctgcggctt ccagcaaaac gcgccctcgc
      541 tccagcaaca tcctcccgac attggtgaat ttggtgcgat ggcccgagcg gtcaaacagc
      601 accacatcca gctcttcttc cagtttttgc atggtgtagc taagtgcgga aggcacgcgt
      661 cccagctcat ccgccgccgc cgcaaaactg ccccggcgat cgatcgcatc cataacccgt
      721 agtgcttcca gcgttaatgc cctttctttg gccatttcgt tctcattcag gaaatttgaa
      781 cataccgggc agaatatctg gctaacaatg tagcgtccag ccccttacca taaaaggaag
      841 taaagagagg tcaagaatta tgattactac ccgaactgcc aggcagtgtg gacaagcaga
      901 ctacggatgg ttgcaggccc ggtatacttt ttcctttgga cactacttcg acccgaaatt
      961 gttaggctat gcctccctgc gtgtgcttaa ccaggaagtg ctggccccag gtgccgcctt
     1021 tcagccgcga acctatccca aagtcgatat tttaaatgtg attctggatg gagaagcaga
     1081 gtatcgcgac agcgaaggca atcatgttca ggccagcgcc ggtgaggcgt tgctgctctc
     1141 tacccagccg ggtgtcagct acagcgaaca caatctcagc aaagacaaac cgttaacacg
     1201 aatgcagctt tggctggacg cctgcccgca gcgagagaat ccgctgattc aaaaactggc
     1261 gcttaatatg ggcaagcagc aattaatcgc ctcgccagag ggtacgatgg gaagcctgca
     1321 attacgccag caagtgtggc tgcaccatat cgtgctcgac aaaggcgaaa gtgcgaattt
     1381 ccagttgcat gggccacgcg cgtatttgca atcgattcac gggaaatttc atgcgcttac
     1441 gcatcatgaa gagaaagcgg cgctgacctg cggtgatggg gcgtttattc gtgacgaggc
     1501 taacattacg ctggttgccg attccccact gcgcgctttg ctgatagatt tgcctgtcta
     1561 gttgttttta cagggagatg atgatgagta aaaaatcggc caaaaagcgc cagccagtga
     1621 agcccgtggt ggcgaaagaa cctgctcgca ccgccaaaaa ttttggctat gaagagatgt
     1681 tgagcgagct ggaagccatc gtcgcggatg ctgaaacgcg tttagccgag gatgaagcta
     1741 ccgcgtaaag acaggttgcc ggatgcggcg aaaacgcctt atccggccta caaaatccaa
     1801 caacattaac gaattaaaca acccgtaggc cggataagat gcgccagcat cgcatccggc
     1861 atcaggtaca aaacacaaca acatcaacaa gttgagcacc ccgtaggccg gataaggtgc
     1921 gccagcatcg catccggcac gatacccaaa acctggcgtt tatctggcct tgctcgccat
     1981 aatctcgata atctgccgat ccgtttgctg catcgaatgg cttgctaacg cacacaggtt
     2041 ggcaatcgac tgctcaacat catgcgccac gatcccttca ttgccggtca cggcggtatc
     2101 atccagcgcc attaacaccg ctttccacgc agccgaagca ctggtcgaaa ccttcatcgc
     2161 gcagctgttc gacgcaccat cgcaaatcat gccgctgaca tcgccgatca tactgctgat
     2221 cgccatcgag atggtttcat aacgcccatc caccagccat gccatcccgg cggcggcccc
     2281 cattgctgcg gtcgttgcgg cacacagcgc agacaaacgc ggtaactggt tatggatgta
     2341 aattgcgctc aaatgagaaa gcatcagcgc acgcgccagc cgttcatcat ccgctccgaa
     2401 gtgttctgct accaccacca caggcattgt tgcggtgatc ccctggttac ccgagccgga
     2461 gttactcata gccggaagcg tagcgccgcc catacgcgca tcggatgccg cgctggtacg
     2521 aatcacaatg gatgaagaga gatctttcgc cagcaaaccg cgctcgcact gtttttccag
     2581 cgtcgcgcca atatgcagcc cccacttacc gctcaaacct tcctgcgata acgcacaatt
     2641 tagcttcgcg gaatcgagaa taaagcggat cgccgcaaac gggacttcat tgacgaactt
     2701 caggatctca gccagcgtcg ttctggaaag caccgtcagc ggagactctt gctcgccctc
     2761 tgccacacac gcctgctggg taaacaccac accattgtgc gtctcaatat gcacaatgtt
     2821 ggtatgcccg ccgacgatgg tgacacacgc ccacttctca ccgttccaga ctttggcgcg
     2881 tgagaagagg atttcattgc aaggttcctg gatcttaacg gagactttcc ccgccgccag
     2941 cagtgctttg gcatcggcaa ttgcctgcgc agttgcgtct ttcagcactt ccagcccggc
     3001 gttggcattt ccacctaacg cccccagcgc cgccgcaatc ggcagcccca ccattcccgt
     3061 gccgggaacg gtgacgccca gaccgttctt catcagattt ggcgaaaccc aggcttctac
     3121 acgttcaacc ggaccttcca gttctgccgc agcaaccgcc gccgccagcg ccagtgaaat
     3181 cggttcagta catcccagcg ccggttttac ttcctcctga acggcgagga tgtaacgctg
     3241 ccataacgga tttaaagtcg aatcaaacat attaaaaacc ttaaaatttc aggtaaatca
     3301 agaaaatgcc aggaacggag aaacacaaag caacaaacca gtgacgataa tcaggtacag
     3361 agacatccct ttgtatttgt gcaatgccgg tactttgtaa accagccacg ccgggatcag
     3421 gcaccctacc atgccgaaaa tcgggctaca gatagaggtg aagctcaaca ccggtgcgtt
     3481 cagtacgatg gcgctccagg ccagcaaaat ggcgaaaatc atgatgccgc gctgaacaag
     3541 attttcgtta atcttctcgg caggcatctt gcgacgcagg atgttcatta cgatcccttg
     3601 cgttgcttcg cgaaagccta aatagacgcc aaagaatgca gtcatgacag caaagatatt
     3661 gagaatgacg ctgaccactt tcacccacgc tgcgccgtca ccgctaataa actgtgcggc
     3721 aatcgccagc gcggaaatat tctgctcata ggcttttacc gcttcgtcat gtcccatcgc
     3781 cagcgtgaac gacacggcgt agaaaaagac ggtgacaaac aaaatgccaa acgcgatatt
     3841 cattgcccgc aatgctttat gccgcgccac ttcaattgat ttttcccgcg agcgataaga
     3901 gatcaccatc gggcttaacg tctggataaa cagaatcgac gtcagggtaa acggcagcgt
     3961 aataatggcg tttttcacca gcagccccag cggcggtagc gaaccgacgt tatacagatg
     4021 ccacattccc accatcgaca cgcccagcgc cgcgaccacc agcagcttgg tcagcaccat
     4081 gccggtcgaa attttgaata acaatttctc gccgcgtgaa gagatcgcca ccagaatgca
     4141 aatcagtacc agaccataaa agggactgtc tgacagcaac ccttccgtca cgccgaaggt
     4201 atgcagatag gaagcactat cgttggtgat ggcggtggaa tagacgaaca tccaaatcac
     4261 cagcattacg aaatagagcg cacctaacag gatgccccag tttttaccta aataaccgct
     4321 aatgacactc gggtaatctt tacattctgg tgattctgcc agcgtattaa taaacaaccg
     4381 ctgaaacaga tacattgccg ggtaaccaat caccgatgag agcaaaaata cccacaatcc
     4441 catcaaaccg acctgcaccg ggagaaaaac aatccccgcg ccaatcgcca tcccgatact
     4501 cataatcacc cagccggtgt cagtactgtc gaatttgatc gcttctcgcc actcgctctc
     4561 actcattcca gcacgacccg ccggggtcga agcgtctgca atgacgcctt tattcgatgc
     4621 aatttccata atttctcgct caatattttg tagggcttat tatttttttc cgagccgcat
     4681 caaggcgata tgcggtctac gtttttgcag gcgagttatt agaataaaga aatgatacgc
     4741 gcgaggtcag agaaaatctt cacaatcaag actttgaaat aacggagatg gataagaatt
     4801 ttctactaaa ttaatcgcaa gagaatattc attctctatt tgcggcagat cacaaaaaaa
     4861 aggtgcacat ttgtgcaccc aaggatgaaa gctgacagca atgtcagccg cagaccactt
     4921 taatggccag tcctccgcgt gatgtttcgc ggtatttatc gttcatatct ttgccggttt
     4981 catacatcgt ctcgatcact ttatcgagtg aaacacgcgg tgccgaggtg cggcgcatcg
     5041 ccatccgcgc ggcgtttact gctttcacgg cattaatggc attacgttca atgcacggga
     5101 tttgtacctg tccggcaacc ggatcgcagg tcagcccaag gttatgctcc atcgcgattt
     5161 ccgccgcatt gcatacctgc gccggactgc cgcccagtag ttcagttaac cctgccgccg
     5221 ccattgaaca ggccacgcca atctccccct gacagccgac ctccgcgcca gagatggagg
     5281 cgttcatttt atacagcgcg ccaatagccc ccgctgccag aaaatagcgg gcaattgacc
     5341 gctcgtttac cggacgacgg aacttatcgt aataagccag cactgccgga ataatgccgc
     5401 acgcaccgtt agtcggtgcc gttaccacgc gcccgccagc tgcgttttct tcactaaccg
     5461 ccagcgcgta catgttgatc cagtcgatga cattcatcgg atcgttagag atgttatcgc
     5521 tagaaaccag ctgacgacgc agcgctacgg cacgacgcgg cacattgagc ggaccaggca
     5581 gcacgccttc agtgttcatc ccgcgttcaa taccgtcatg catcacttgc cagatacggg
     5641 caaaccctgc gtcaatttcc gctttgctgc gcagcgccag ctcgttgtgc atcatcaggc
     5701 cagatataga caggccgttg taatcacaca ttttcagcag ttcacctgct gagtggaaat
     5761 cgtaaggtac ggacgtttcg acatcgtgcg acaggccgaa gtgttcttct tcgacaataa
     5821 acccgccgcc gacagagtaa taggttttac ttaatagcgc ttcctgtccc ttccaggcag
     5881 tgatccgcat tccgttctca tggcgaggca acatttcggg atggaagata atgttctttg
     5941 ctacaggaaa atcaacaata tgcgcaccag atgccactgg cagccgcccg ctgcgcgtta
     6001 ctaactctat aaatgcaggg atctcatcaa tgacaacatc ctgcggactg tttcctgcca
     6061 gtcccatgat aatggcgaca tccgtggcat ggcctttgcc cgtcagtgac aacgacccat
     6121 acagatcgac cacaatatgg ctcgtcgcgg ttaataagcc gctactttcc agccgatcaa
     6181 taaaactttt tccggcattc attggcccca cggtatgcga actggaggga ccaatcccaa
     6241 ttttgaaaat atcgaatgca ctaatcatat ccacaccctc ggattgccgt tcagtgaagt
     6301 ggagcggaac gaccttgcga ccgtcccgct cacgaggctt tacgcactac gtactgcgat
     6361 agcttcaatt tccagcttca catctttcgg caaacgcgca acctgcacat agctccgggt
     6421 cgggtaagtc gcctgatgct catcgaagaa ctgcttatag acttcattga tggtggcaaa
     6481 atcattcaga tcggtgatga acacggtcat cttgatgata tcgcccacgc tcagcccggc
     6541 agcaaccacg atcgctttga cgttttcgag gcttaaacgc gcctgatctt gcacatcagc
     6601 cgggatctca ccggtctgcg ggcaaaccgg aatttgcccg gaggtgaaga ccatgctgcc
     6661 taaatcaacg ccctgaacat aagggccgat tgcgcctggg gcacgttgcg tttcgataat
     6721 ctttttcata catcctccgg cgtcagagcg cctgggtaaa agtacgtgaa ataacatcct
     6781 gttgctgttc acgggtcagt gcgttgaagc gcacggcgta gccagagaca cggattgtca
     6841 ggttaggata tttttccggg tgctcgatgg catccagcag catttcccga ttcattacgt
     6901 tgacgttgag gtgttgaccg ccttcgacat ccgcttcgtg gtggaaatac ccatccagca
     6961 ggccgacaag gttggtttta cgtactggat cttctttgcc cagcgccgca ggaacgattg
     7021 agaaggtgta cgagatccca tctttggcgt aggtgaacgg cagtttcgcc accgacgtca
     7081 atgaggccac agcacctttg cggtcacgac catgcatcgg gttagcgccc ggcgcgaacg
     7141 gtgttccggc gcgacgaccg tccggcgtat taccggtttt ctggccgtac accacgttag
     7201 aagtgatagt cagaatcgac tgggtaggga cggcgttgcg ataggttggc agcgctttaa
     7261 ttttcttcat aaagcgttca accaggtcgc aggcaatgct gtctacgcgc tcgtcgttgt
     7321 tgccgtactg cggatattca ccgtcgattt caaagtccac cgccaggccg ttttcgtcac
     7381 ggattggttt cacgcgggca tatttgatgg cagacaggga gtccgtcgcc accgacaggc
     7441 ccgcgatgcc gcatgccata gtgcgataga catcacgatc gtgcagcgcc atcagcgaag
     7501 cttcgtagct gtacttgtcg tgcatgtagt gaatgatatt cagcgcgctg atgtactgca
     7561 ccgccagcca gtccatgaag tgatcgaggc tgtccatcac tttgtcgtaa tccagcacgt
     7621 cgtccatcag cggtgctgtt ttcggcccga cctgaatctt cagcttctcg tccaccccgc
     7681 cgttaattgc gtagagcagc gttttcgcca ggttagcgcg tgcaccaaag aactgcattt
     7741 gcttaccaat caccattggg ctgacgcagc aggcaatcgc gtaatcgtcg ctgttgaagt
     7801 cagtacgcat cagatcgtca ttttcatact gcaaggaaga ggtgacgatc gacacctgcg
     7861 cggcatattt tttgaaggca atcggtaatt cttccgacca aagaatggtc aggttaggtt
     7921 ccggtgccgg ccccatagtg tgcagggtgt gcaaatagcg gaaggagttt ttggtcacca
     7981 gcgtacgacc gtccagcccc atcccgccga tcacttccgt cgcccagatt gggtcgccgg
     8041 agaacagcga atcaaattcc ggtgtacgca ggaagcgtac catacggatc ttcatgatga
     8101 agtgatcgat cagttcctgt gcctgctgct cattgagtac gccagcttta aagtcgcgct
     8161 caatgtagat atcgaggaac gatgccgtgc ggcccagcga catcgcgccg ccattttgcg
     8221 atttcactgc cgccagataa gcgaagtaga gccactgcac cgcttcctgc gcattctgcg
     8281 ccgggcgaga gatatcaaag ccatatttcg ccgccatttc ctgaatctgc aacagcgcat
     8341 gacgatgctc tgccagctcc tcacgcagac ggatggtggc ttccagatcc tcgccttttt
     8401 ccagacgaga ctggagatcg gcaaattgca gttcgcgttc acgtaccaga taactgatgc
     8461 catacagcgc tacgcggcga tagtcaccga taatgcgccc acggccatag ccatctggta
     8521 aaccggtcag cacgccagat ttacggcagc gcagcatatc cggtgagtaa acatcaaata
     8581 cgccctggtt atgggtttta cgcagatcgg taaacagata ttcaaattca ctgtccattt
     8641 cacggccata ggcgtggaat gaacttttaa tcatattaat gccaccgaac gggtgtagcg
     8701 cacgtttcaa cggcgcatcc gtttgcaggc caacaatttt ttccagcggc tggttaatat
     8761 atcccgcatc atgagcggta attgtggtgg caatattggt atcgaaatca accggcgcgt
     8821 gggttgcgtt ttcgatacgg ataccttcca ttactttttc ccacaattcc gtggtggcag
     8881 gcgtcgcttc ggcgaggaaa gattcatcgc cttcatacgg tgtatagtta tgttgaataa
     8941 aatcgcggac attaatttcg tttttccagt ccgtaccttt aaagccaagc catgcgtcgg
     9001 cgtacagctt atcgctggta tcaatatcta ccttcatgaa aaataatctc tctacaatac
     9061 ttcaactaaa ttatgcaaat tctgcaggcg cgttaacttt gcctaaatga atggcatcca
     9121 aagcaatcat tttttcttcg ttagtcggaa taacagcgca aatgacacgc gcattttcac
     9181 tggaaacaat tcgctcacca caggagttag agcgattatt catttctgta tcaatctcta
     9241 cgcctaatac agccaaatgt tccatgacca gacgacgaat taagcttgaa ttctctccta
     9301 ttccgccggt gaatataatt ccatccaggc gacgtaatga agctgcgtgt ccggcaatat
     9361 gacgggcaat tcggtgaaca aaggttttaa ttgccagttg cgcgcgttcg tgaccttcat
     9421 gccaggcttt ttccagaaca cgtaaatccg aagaaagacc ggaaatacct aataatcccg
     9481 actctttatt cactacgcgt tccaggtcac ccaggctctg gttggtttgg ctggcgaccc
     9541 aggacatcgc accaaagtcg acatcgccac tgcgggtacc catcatcaag ccttccagcg
     9601 gcgtcattcc cattgaagta tcaacactct gaccgttgcg aaccgcgcag attgacgcgc
     9661 cattgccaag atgcgccaca accaggccgg agtcatcttc cgccagattc agcagcgaat
     9721 gtgcgcgctg ggaaacatag cggtgcgacg tgccgtggaa accataacgg cgtacaccta
     9781 actcttcata atatttccac ggcaggccgt ataaataagc ttccggagcc atcgtctggt
     9841 ggaaactggt atcaaatacc gccacctgag ttacgcccgg aaataattgc tgcgccgatt
     9901 caataccact taaattggcg taattatgca ggggtgccag tggagaaacg cgacggatat
     9961 tatcaatgac ttcatcggta ataatggcgg actcggtaaa aatactgccg ccgtgagcga
    10021 tgcggtggcc aattaaggcc acactgtcat ttaaattccg tttttccagt tcaaatgcaa
    10081 ttgccttcaa tgcaccttcg tagctgtggt gagccagcgg tgctggctct cccccattta
    10141 cggataagaa tgcattttcc gagttaatac cgtcggcaat acctgacatt aatacttcac
    10201 agtcgctggc atcgagtacg gaaaacttaa tcgaagacga accacagtta ataaccaaaa
    10261 caaccggaaa ttcattcatc tcttttctca tcctgagtta cggattaaaa cagtttgtat
    10321 acgatgttca ggatggtcag cagaccaatc acggtaacaa acacgttatc cagacgacca
    10381 cggtatttcg ccagagacgg cgctttacgg atggcataca tcggcaacag gcacagcagg
    10441 gatgcgataa tcggtgcgcc catggcttca ataaggtcga ggatgttcgg gttggcgtag
    10501 gcaacaaccc aggtggagcc catgatgaag atcatgctga tagtgttcag tttacccagc
    10561 gacactttag ttttgtcgcc tttataaccg aacttcagaa tcagaccatt caagccttcc
    10621 agcgtcccca gatagtgacc gaagaaagat ttgaagatag ccacgagtgc gatgatggaa
    10681 gccgcatatt ccagtgtaat tgcgaacgtt gttttggtac cggtcatgga cgcaaagtgg
    10741 ttagccagat aagaaagcac tggaatattc tgcgctttgg cttccgccat gttggccgga
    10801 gacagcgtaa acaggcagct aaaggcaaag aacatcacca ctgcaaccat cagcatgctg
    10861 gcacgagaaa tgatttggga acatttacgt tcggtgaagt cgcgaccgaa gtctttctca
    10921 tactcttcac gtttagaaac cacgaaggaa gagacgattg gcgagaagtt aaaggagaaa
    10981 accatgatgg aaatccccag ccagacagtg atcaggatac cgtcatgacc ggttaacgac
    11041 agcgaaccga ggtcaacctg gtcgataact gcagagttcc agtaagggat cagcgacaga
    11101 gaaatcagca ccaggctggc gataaacggc cataccaggt agctcatcac tttaaccatc
    11161 agatccttac caaaccagat gacgaaagcc atcagcagca acaggaacag cgccacaaag
    11221 ccgcgattca gcggtgcaaa tccgagctgg ttttcccaga acgtcataaa ggtattggta
    11281 atagtaacgc cataaatcca cagcagtggg caaatcgcga agaagtacag gaacgtgata
    11341 accacgccgc cagttttacc aaaatgctct tccaccgttt ccgtaatgtt gccggaaggg
    11401 ttagagccag aaagacacag acgcgccagc gcccggtggc aataaaatgc gatggggtat
    11461 gccaatacca acatcagaag aatcgggatc agtccgccaa aacctgcgcg gatagggaag
    11521 aacagcaccc cggcgccgat tgccgtacca aacaagccta acgtccatgt ggtatctgat
    11581 ttacgccagg acgattgttt tgtctggctg gatacaatgc tatctgaagt actcatatcc
    11641 tatcctcaac gaattaatta agcgtcaacg aaaccggtga tttgagagac gcgagaaaga
    11701 tcgatattgc cgccggaaat aatactgacg gtttttctgt tttgaatata ttggtctaat
    11761 ttaccgctta ataatgcagc acatgccaga gcgcctgcgc cttcggtgac gactttattg
    11821 cgctgaatta aggcaatcat actgtttctg atttcgtctt cgctgaccag cacgatgtca
    11881 tcgactaatt cacgaacgat ttcgtaagtt aaattacccg ggcgggagac atcacaacca
    11941 tccgccaggg tgccggtagt tcggtgcgtg gttatttctc cggagtggaa agaagccgcc
    12001 atgccgtgaa cgttttcaga ctgtacgcca ataacacgaa tggtcgggtt aatagattta
    12061 attgccaccg caataccagc aattaaaccg ccaccaccaa ttggcacaat cacgttatcg
    12121 acatcataga gatcttccat aatttccaga ccaatcgttc cctggccagc aatcactttc
    12181 ggatcatcgt aaggtgggat aaaaatacgg ccttccattt cgacaatttc gctcacttta
    12241 gcgatagtgt cgttgaaatt atcaccatgc agaacgactt ctgcggagta gtcgcacgtt
    12301 gccgctactt tggattttgg cgcacctttt ggcatcacca ctttaccgtc gatacccagc
    12361 atcgcgcagg agagcgagac cccttgcgca tggttgcccg cagaacaggc cactacacct
    12421 ttacgttttt ccgcatcagt cagtgaactt aatttattaa atgcgccacg aattttaaat
    12481 gaacccgtac gctgcatatt ttcaaacttc aggaatattt cacctttgca acgttcacta
    12541 aaatagtttg agcgaggcat gcctgtttta taaattcgcc cagccagtcg ttgtttcgct
    12601 tcaataatgt catcaatagc aaccggcaga tcgtatgtaa tatgcattat aaaacctctt
    12661 cgcctgaatt aaatatgtag gtaaccgtaa ccgacacctg caaaacagac aggtggatta
    12721 tttatgttta aataaaaaca aataactaac caacttctat taattgcctt cgtctacacc
    12781 cattataaga tgaatactct ttggctaatt ccaccaaaac cgatgctgct tttttaatac
    12841 gataattttt cgaccatacc gcggcatatt gtgccacagg taatgtttct tcaaccggaa
    12901 tagtaataaa ttgatttgaa ccaaaaggtg acgtcatatc acaaggaatt acagttaaga
    12961 aatcagcatt gagaacaaga ttataaattg tcacgactga gtcggtttta acgatgtttt
    13021 caatactgat gccatttctt tgtaacgtag taagaagctc gctgtagtac cccatattgg
    13081 tttgtggcaa cacccactgt tcgttcttca acgactccag cgtggtggtg ccggtgcatg
    13141 ttcgggactt actggctacc agcacaaact cggactcgaa cagcggctca acatgtaaat
    13201 cctgaagctt catttctgca cttaacgtac caatcgcaaa atccagtcga ccgtcgcgga
    13261 ttgccggtaa gaacgaagac agttgcgctt catacataga aacctgcgct ttcgggaaca
    13321 cctctttgaa cttgttgatc atccctgaca taaaagtaaa accaatcaat gaaggaaaac
    13381 caaatgagac ttccaccacc gcctcagaag acataccgct tatctcatta accatatttt
    13441 tcatttcacg ggtaatggat tcggaacggg agagtaacaa ttgaccggca ggtgttaatg
    13501 ttacaccggt atttttccgc accactaatt ccacaccaaa ataatcttca atatcgttaa
    13561 tgattttact gacggccggt tgagttaacc ctaattcttt tgcagccgag ccgatagagc
    13621 cacttctaat gacttcctga aagactacca ggtgctgcgt tttcggaaga agaatagtgc
    13681 tcataacgac ctacgttaat tacctcattg acggcatgaa gtgtatcaaa atgaaatgaa
    13741 caggatatgt gcgaccactc acaaa
//
LOCUS       DAFNBJ010000129        13719 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000129 DAFNBJ010000000
VERSION     DAFNBJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.129"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(164..376)
                     /gene="cspH"
                     /locus_tag="L2X49_003626"
     CDS             complement(164..376)
                     /gene="cspH"
                     /locus_tag="L2X49_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBN4798647.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            662..874
                     /gene="cspG"
                     /locus_tag="L2X49_003627"
     CDS             662..874
                     /gene="cspG"
                     /locus_tag="L2X49_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBN4798648.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            885..1073
                     /locus_tag="L2X49_003628"
     CDS             885..1073
                     /locus_tag="L2X49_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBN4798649.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1048..1278
                     /gene="ymcE"
                     /locus_tag="L2X49_003629"
     CDS             1048..1278
                     /gene="ymcE"
                     /locus_tag="L2X49_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBN4798650.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            1268..1441
                     /locus_tag="L2X49_003630"
     CDS             1268..1441
                     /locus_tag="L2X49_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBN4798651.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1490..2563)
                     /locus_tag="L2X49_003631"
     CDS             complement(1490..2563)
                     /locus_tag="L2X49_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBN4798652.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHVAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(2635..5379)
                     /gene="torS"
                     /locus_tag="L2X49_003632"
     CDS             complement(2635..5379)
                     /gene="torS"
                     /locus_tag="L2X49_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HBN4798653.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTEGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITIEMLKTSGAQIVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5462..6490
                     /gene="torT"
                     /locus_tag="L2X49_003633"
     CDS             5462..6490
                     /gene="torT"
                     /locus_tag="L2X49_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBN4798654.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6463..7155)
                     /gene="torR"
                     /locus_tag="L2X49_003634"
     CDS             complement(6463..7155)
                     /gene="torR"
                     /locus_tag="L2X49_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBN4798655.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7285..8457
                     /gene="torC"
                     /locus_tag="L2X49_003635"
     CDS             7285..8457
                     /gene="torC"
                     /locus_tag="L2X49_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBN4798656.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDMPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8457..11003
                     /gene="torA"
                     /locus_tag="L2X49_003636"
     CDS             8457..11003
                     /gene="torA"
                     /locus_tag="L2X49_003636"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBN4798657.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            11000..11599
                     /gene="torD"
                     /locus_tag="L2X49_003637"
     CDS             11000..11599
                     /gene="torD"
                     /locus_tag="L2X49_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBN4798658.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(11692..11997)
                     /gene="cbpM"
                     /locus_tag="L2X49_003638"
     CDS             complement(11692..11997)
                     /gene="cbpM"
                     /locus_tag="L2X49_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBN4798659.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11997..12917)
                     /gene="cbpA"
                     /locus_tag="L2X49_003639"
     CDS             complement(11997..12917)
                     /gene="cbpA"
                     /locus_tag="L2X49_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBN4798660.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            13177..>13719
                     /locus_tag="L2X49_003640"
     CDS             13177..>13719
                     /locus_tag="L2X49_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798661.1"
                     /translation="MSSNIHGISCTANNYLKQAWNNIKNEHEKNQKYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECDSLEKEFEEMQNDNDLSLFLRTLRTNDTETYSGVSE
                     GITYTIQYVRDIDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEWINTPGISDLDT"
BASE COUNT         3395 a         3399 c         3562 g         3363 t
ORIGIN      
        1 tactataact ggctgttaaa tggcatttat aactattagg tgcagagata ttcgcttaaa
       61 tggattagtt tctttgtgga aaagtaaccg atgttataat gtatgatgtt gactattgca
      121 ctattccaat aaaaccagtc agctttaaac aagcagcgtc atattaagag agataaacat
      181 ttgccgctgt tggtcctcgc aggccattta cgcggcaaaa ttccacgcgt aatcctggta
      241 taagcacttc tgcgtcgcgg ggagtgaatg cggaaatatg gacctggact tctttacgac
      301 cgtcggaggg gataatgaat cctttgccgc ttttgcgatc aaaggttttg acaattcctg
      361 tcattttacg ggacaaacaa attccttact gaaaatactg tgccgcacta tacggggtta
      421 ataaaataaa gccagcgata tttaagaccg ccggacggct aaaataaaat ttgcttaatc
      481 tcaattatca tgcgttaata gctgcgtcgg tttgaaagac agacagcata caaagtagtt
      541 tactaaagca gttctcatta tcaggcatta tccctttctt ttgagtctct ctcctgaaca
      601 ctaagtagtt tctgtattaa agccctgttt gccgaaaggc ccaaaatgaa ggaagtaaaa
      661 tatgtctaat aaaatgactg gtttagtaaa atggtttaac gcagataaag gttttggctt
      721 tatcactcct gatgatggca gcaaagacgt tttcgtccat ttcaccgcca ttcagagcaa
      781 tgaattccgc acgctgaacg aaaatcagaa agttgaattt tctattgagc aggggcaacg
      841 tggccccgcg gcagcgaacg ttgttacgct ctaaggttgc cgttattact caacatctcc
      901 atttccgctg tccatgttgt catggttcac agtaccgtac atcggcattc gatgtgacgg
      961 agcgaaaccc tttgggcgct aagtgtattt tttgtaaatc gacgatgatc acctttgata
     1021 acgtcgcgct gcaaatacgc acggaccatg cgccgctgga tttcacaaaa taatatcagg
     1081 ctccctcgtg gagccttttt tatatctgcc ttatttttct tcaacgctgt atgtatagta
     1141 agcgataacc tgttgattat tgaatctttc ggggagatgg cttataacat ttcttacctg
     1201 accagggtac cgggaaccaa caccttactg gcgtgttgct gtctttcaag accagacgag
     1261 gttaacagtg aatattgaag agttaaaaaa acaagccgaa acggaaatcg ctgactttat
     1321 cgcgcaaaaa atcgccgagc tgaacaagaa tacagggaaa gaagtctctg aaattcgctt
     1381 caccgcacga gaaaaaatga ccgggcttga aagttatgat gtcaaaatca aaataatgtg
     1441 attttgtgaa catcaccccg tgcgaggtga tgttccgctt gttgctaatt tagtgaccaa
     1501 tcattggcgc ttgtggaatt aagcgtcggt acaattcctc cggcaccggg ctttgccata
     1561 ctcccgcata cattgcgtaa ccaatcaccg caaacataat ccccagaacc agtagcgtca
     1621 ttaaccagcc agacaacgca aaagcttttt tatttgccgc aggtttttgc agtgaaaagg
     1681 tcaatgttga ggctaccgga catgactcta cgcaagtcat acagccggta cattccactg
     1741 ttcgtacctg aattaattta tctaccggga tccgtgatgg gcaatttttt gcgcatttgc
     1801 cacagtcgat acaactttcg gcattgcgac gaatcttaaa cggcgacaat agcgaaacca
     1861 cgcccatcag cgcgccatat gggcaaagat aacgacacca ggcatggcga ataaacaggc
     1921 tggcaatcag caaaacggtc acgctgatta atgtcgcggt ccccatatga cgaaagaaat
     1981 cgagcatttt tacgtccatc accacgctgt agggcgacaa cataaaatag tgaatcgcct
     2041 gagcgggcat caataacgcg atatagagaa aaaaactcaa cagcaaatac ttcacgccgc
     2101 gcagaggaat atccagccag cggggaagga cacattgccg accaaacagt ttgttaccga
     2161 gatcgccgat taattcagaa agcgtaccaa ccgggcataa ccatgagcaa aaggcctttt
     2221 tgagtaatag actgatgacg ataaaagcga ccaataacag catcgcggcg acgtggacgg
     2281 acggtaactg acctgttgta aggctatatt tcagattcat cagaccggca atcggtagcc
     2341 agccttcgat acctcccggt ctggcgacaa atgtcgtgct acttgccgtt tcgtaatagc
     2401 gcacccaata ccagaacgtg atggcaatat aaatattcat tgccaacagt aataattgcg
     2461 tcgctttacg ccaggtcgtg gcattacgcc agtcattcca cggtaatttg ccgcccgtcg
     2521 tgcctggccg ccgctgccag cgggttcttt tattctctgc catgattttg ccagtccgtt
     2581 aagttgtata ccaaatgcca ctattctagt tgttcttaac tggctgatat tgattcaaat
     2641 cgcgttcagg tctttcttat gcaaccatgc ttccagagcg gcaacactgc gtgtaatttc
     2701 ttcgtgtgga aggggggcag ataatggctg ctgctccagt tgtgcgcata gctggctggc
     2761 gatacgcatt cccagactcg agcaactgct ttttagctga tgcgcggcac gctttatttt
     2821 ctcgctatcc tgactggcgc gggcaatgtc gatttcatcg agaagcggca gggcatgttg
     2881 tgtaaataat accagccatt cgtggatctt ctccgtcccc attaactgag catcttcatt
     2941 gagttgcgat acatccagcg attgatcatt attgacttgc agttggagat agtgcgccag
     3001 taactgaccg agcacttcac gcggcaccgg tttagggata atcccgcgga ataatgaact
     3061 ggtacgctgg cgcagcgttt cgtcaatgac atgggcgcta aagccaatca aaaccagcga
     3121 cggatattgc tgtgccagtt gtcgggcaag cgtaatgccg tcgatatccg gcagatcaaa
     3181 atccaccagt gcggcagcaa acggttcgct attttgcagt gtctctaaag cctgcgcggc
     3241 attgccaaca gcaacaatct gcgcaccact ggttttcagc atctcaatgg taattcgctg
     3301 ggttagcggg ttatcttcaa ttaacagcaa acgtaaaccg tcaagacgca ccgcctgatt
     3361 gactgttttt ggcacgggtg ccgtggcaac acgtaacggc aagcgtaaac aaaaacagct
     3421 tccaacctcc ggcgtgctgg tggcgctcag ttcgccgccc atcgcctggg ccagacggct
     3481 actgatagtc agtcccagcc cggtgccgcc gcgtttgccg cttacctgca caaatggctg
     3541 gaagatttct gccagtttcg cgggatcaat accgcagccg ctgtcttcca cttcgaccag
     3601 ccattgctcg ccttcagtgc gactacgcag gataatgtac ccttcgtcag taaaacgcag
     3661 ggcgttgctc aacaggttgg ttataacctg acgaatacgt cgtggatcgc ccattaacgc
     3721 gcacggcata tcatcggcaa ttgccgttgc caggcgaatc gggcgacctt tcacccgtcc
     3781 gctcattaat tgcagggtac tttccagcag cgggcgcggt tcaaagggct catcgctgac
     3841 cgaaacattc ttgccacctg cttcgatagc ggaataatcg agaatatcgt tgaggatggt
     3901 cagcaacgat tcaccagagt cagtaattgc ccgcaaatca tcacgctggg cgttaagtgc
     3961 ggggttatct gccagtagct gtgcggtgcc gagaataccg tacagcggtg tgcggatctc
     4021 atggctcatc gccgccagaa acgccgattt tgcctggctg gctttttctg cttccgcccg
     4081 tgcctgtcgg tgttctatca ccagttcctg caattcagct gtacgcgctt tgacctgcgc
     4141 cgccagctgt tcgcggtggc gattcagtgc atgaacattg ctgcgaaacg catccatcag
     4201 ccgcccgatg gtatccagct cccgtacgcc agcggtttcc gggaaagggg agtcaatatc
     4261 accgtccagc agccgttgca gcgcctgcgt ttgttcggca agtggacgcg tgactgagcg
     4321 ataaaccacg cgccagagga tcagaatcag tgcgcaaagt gaaaccatcc ccagcaataa
     4381 caggctgtat tgcccgcgtg cactggcttt ttccagatgt gccagtccat gctgattgcg
     4441 cagctcaatg gtgtcgacca gctgactgac ttcgctacta aactgcgcga actgggcgat
     4501 attattttgg gcgagagttt gcaggtgatt gctgatttca ctgtcctgct ggaacagcgc
     4561 caacagatcg ctatattggc taacggtagt taacgttgtt gcgacctgcg cacgaacacc
     4621 cggatcttca atgcgtattt gccgacgttg cagaattttc accgcattat tgagctgctt
     4681 ttccagcgtt ggcgcatttt tctggatctg ctccagcccc agattcatca ccatttgctg
     4741 cacccgcagg gcgctaaggc gcagttcatt catctggtta acatactcaa gatcgatatc
     4801 aatcagccga tcgagtgcac tttctgcggc ctgacgctga tcttgttcga tcaaatcgta
     4861 aatcccggcc tgggtcgctc cggcggaggt tgccgcatta ttcgcctgac cttgcgccag
     4921 gcgtgcgatc tcatcggcgg cagcgactat ctgctgactg agttgctgtt gttgctggcg
     4981 cagttgcaaa cgctgcccca ccagttcccc ttgctgacgt aacgaacggg agatctcctg
     5041 ctcctgttgt tcaatagcgg tggtatcaaa accttgttcc cgtaacgctt gcagcaacgc
     5101 attaatcttc aggctttgtg cggtgagcat tcgcccctgc gcctgccaca tcttttcgtt
     5161 atcggcactg gtcaggttct gcgcggcgaa aagttcccag gcgctggctt cgctcaactg
     5221 gcgcgccata ttcatggtag gaatcaatgc ctgagtattg tctttttcca cctggctgat
     5281 aaagcgcagg ttgtaccatc ccaccagggt actggtcagg gttaacagcg ccatcagggc
     5341 aaagcccatc cagagtcttc gggtcagggt taaattcacg gtcggtgcac tttaggtgaa
     5401 aaaggttgag tcgcaaagcg gaatgcatct agcataaagc cttattattg atgaggctat
     5461 catgcgcgta ctgctatttt tacttctttc ccttttcatg ttgccggcat tttcggctga
     5521 taacctgttg cgctggcatg atgcgcagca tttcacggtg caagcctcta cgccgcttaa
     5581 agccaaacgc gcatggaaac tgtgcgcgct ttatcccagc ctgaaagatt catattggtt
     5641 atcgttgaac tatggtatgc aggaggctgc tcgccgctac ggtgtggatt taaaagtgct
     5701 ggaggcaggc ggctacagcc agttggctac ccagcaagca caaatcgacc agtgtaaaca
     5761 gtggggcgca gaggccattt tgctcggtag tagcacgacc tcatttcccg acctgcaaaa
     5821 gcaggtagca agtctgccgg tgatcgaact ggtaaatgct attgatgctc cccaggtgaa
     5881 aagccgcgtt ggtgtgccct ggtttcagat gggctatcaa ccggggcgat atctggtgca
     5941 atgggcgcac ggtaaaccac tgaatgtgct gttgatgccc ggacccgata acgccggggg
     6001 cagtaaggag atggtcgagg gttttcgcgc agccattgcc ggaagcccgg tgcgtattgt
     6061 tgatattgcg cttggtgata acgatattga aatccagcgt aacctgttgc aggagatgct
     6121 ggaacgccat ccagaaatcg acgtcgttgc cggaacggcc attgcggcag aggcggcaat
     6181 gggggaaggg cgtaacctga aaacgccgct taccgtggtg tcgttttatc tttcacatca
     6241 ggtgtatcgc gggctgaagc ggggaagagt gattatggct gccagcgatc aaatggtctg
     6301 gcagggggaa ctggcagttg agcaggccat caggcaatta caggggcaat cggtgtctga
     6361 taatgtcagc ccaccgattt tagttctgac gccgaaaaat gccgaccgag aacatatccg
     6421 ccgctcgctg tcaccggggg gatttcgtcc ggtctatttt tatcagcaca catcagcggc
     6481 taagaaataa ccttcaccat gttgcgtcac cagtaaatcc gcgctgagtt tatgacgtaa
     6541 acgacgaatt aacacatcga cggtgcgcag gtcagggttt tccacccgac gcgcagaaag
     6601 catacgtagc agacgttcac ggctgagaat ttcgcccgga ttcgtcacaa atgccaccaa
     6661 catttcatac tctgcgcggg tcagtttaat cggctcgcca tcccgctcca gcgtatggcg
     6721 cgacacattc aggcaataac cggcaaagcg atagcagttg tcctgagtgt gcggttgagc
     6781 ttgtcgcgcg aggtcgattc gccagagcag atttttcacc cgtactacca gttcgcgcag
     6841 ttccagcggt ttggtgacgt aatcgtctgc gcccatttcc agcccaacaa tacggtcaat
     6901 ccgatcgctg cgtccggtaa ccagaataat ccccaccgtt gagcgttctc gcagggcgcg
     6961 ggttaacatc aggccatttt catcgggtaa gttgatatcc agcagaatta aatctaccgg
     7021 ctgattctgc ataatttccc gcagcccggc accgctcgct gtaacggaaa cggtataccc
     7081 ctcctgagtg aagtaagatt gtaatcgcgc ctgggtaacc ggctcatctt caacaataac
     7141 aatgtgatgt ggcatcagag ggttttactc attctgttca tatctgttca tattctgccg
     7201 taagccgttc atcctgacca gtgccgctgt tcatatttgc tcattaagat cgcttcacta
     7261 aaccataatt ttacaggggc tattatgcgg aaactctgga acgcgctacg ccgacccagt
     7321 gctcgttggt cggtactggc gctggtcgca attgggattg tgattggcat tgcgctgatt
     7381 gtattgccac acgttgggat caaagtcacc agcacaaccg aattttgtgt cagttgccac
     7441 agtatgcaac cggtgtatga agaatataaa cagtcggtgc atttccagaa cgcctccggc
     7501 gtgcgagctg aatgccatga ctgtcatatc ccgccggata tgccaggcat ggtgaagcgt
     7561 aaactggaag cgagcaatga tatctaccag acctttattg ctcactccat tgatacacct
     7621 gaaaaattcg aagccaaacg cgcggaactt gccgagcgtg aatgggcgcg aatgaaagaa
     7681 aacaactcgg caacctgccg ctcctgccat aactacgatg cgatggatca tgcgaagcag
     7741 catcctgaag cggcacgtca gatgaaggtg gcagcgaaag ataatcaatc ctgcatcgac
     7801 tgtcataaag gtattgccca ccagttaccg gatatgagta gcggcttccg taagcagttc
     7861 gatgagctgc gcgccagtgc taatgacagt ggtgacacgc tgtactctat tgatattaag
     7921 ccaatttatg cggcgaaagg cgataaagaa gcctctggtt ctctgctgcc tgcttcggaa
     7981 gtgaaagtcc ttaaacgtga cggcgactgg ctgcaaattg aaattaccgg ctggacggaa
     8041 agcgccggac gtcagcgtgt actcacccaa ttcccaggta aacgcatctt tgttgcctcg
     8101 attcgtggtg atgtgcagca gcaggtaaaa acgctggaga aaaccaccgt tgccgacacc
     8161 aataccgagt ggagcaagtt gcaggccact gcgtggatga agaaaggcga catggtgaac
     8221 gatatcaaac cgatctgggc ttatgcggat tcgttgtaca acggcacctg taaccagtgc
     8281 cacggcgcac cggaaatcgc ccactttgac gctaacggtt ggatcggcac gctcaacggc
     8341 atgattggct ttaccagtct cgataaacgt gaagaacgca ccttgttgaa atatctgcaa
     8401 atgaatgcgt ctgacaccgc aggtaaggct cacggcgata agaaggaaga aaaataatga
     8461 acaataacga tctctttcag gcatcacgtc ggcgttttct ggcacaactc ggcggcttaa
     8521 ccgtcgccgg gatgctgggg ccgtcattgt taacgccgcg ccgtgcgact gcggcgcaag
     8581 cggcgactga cgctgtcatc tcgaaagagg gcattcttac cgggtcgcac tggggggcta
     8641 tccgcgcgac ggtgaaggat ggtcgctttg tggcggcaaa accgttcgaa ctggataaat
     8701 atccgtcgaa aatgattgcc ggattgccgg atcacgtaca caacgcggcg cgtattcgtt
     8761 atccgatggt acgcgtggac tggctgcgta agcgccatct cagcgatacc tcccagcgcg
     8821 gtgataaccg ttttgtgcgc gtgagctggg atgaagccct cgacatgttc tatgaagaac
     8881 tggaacgcgt gcagaaaact cacgggccga gtgccttgct gaccgccagt ggttggcaat
     8941 cgacggggat gttccataac gcttcgggga tgctggcgaa agctattgcc ttgcatggta
     9001 atagcgttgg tacgggcgga gattactcta ccggtgctgc gcaggtgatc ctgccgcgcg
     9061 tggttggctc aatggaagtg tatgaacagc aaacctcctg gccgctggta ttgcagaaca
     9121 gcaaaaccat tgtgctgtgg ggctctgatt tgctgaaaaa ccagcaagcg aactggtggt
     9181 gcccggatca cgatgtttat gaatattacg cgcagctaaa agcgaaagtc gccgccggtg
     9241 aaattgaggt catcagcatc gatccggttg tcacatccac ccatgagtat ctggggcgcg
     9301 agcatgtgaa gcacattgcg gttaacccgc aaactgatgt gccgctgcaa ctggcgctgg
     9361 cgtatacgct gtacagtgaa aacctgtacg acaaaaactt cctcgctaac tactgtgtgg
     9421 gttttgagca gttcctgccg tacctgctgg gtgagaaaga cggtcagccg aaagatgccg
     9481 catgggctga aaaactgacc ggcattgatg ccgaaaccat tcgtgggctg gcgcggcaga
     9541 tggcggcgaa cagaacgcaa attattgctg gctggtgcgt gcagcgtatg cagcacggtg
     9601 aacagtgggc gtggatgatt gtggttctgg cggcgatgct ggggcaaatt ggcctgccag
     9661 gtggtggctt tggttttggc tggcactata acggcgcagg cacgccgggg cgtaaaggcg
     9721 ttattcttag tggtttctcc ggctctacgt cgattccgcc tgttcacgac aacagtgatt
     9781 acaaaggcta cagcagcacc attccgattg cccgttttat cgatgcgatc ctcgaaccgg
     9841 ggaaagtgat caactggaac ggtaaatcgg taaaactgcc gccgctgaaa atgtgtattt
     9901 ttgccggaac taacccattc catcgccatc agcagatcaa ccgcattatt gaaggctggc
     9961 gcaagctgga aacggttatc gccatagata accagtggac ctcaacctgc cgctttgccg
    10021 atatcgtgct gcctgcgacc acgcagtttg agcgtaacga tctcgaccag tacggcaacc
    10081 actccaaccg tggcattatc gccatgaaac aggtggtgcc gccgcagttc gaggcgcgca
    10141 acgactttga tattttccgc gagctgtgcc gtcgctttaa tcgcgaagaa gcctttaccg
    10201 aagggctgga cgaaatgggc tggctgaaac gcatctggca ggaaggtgta caacaaggca
    10261 aaggacgcgg cgttcatctg ccagcgtttg atgacttctg gaataacaaa gagtacgtcg
    10321 agtttgacca tccgcagatg tttgttcgcc accaggcatt ccgcgaagat ccggatctcg
    10381 aaccgctggg cacgccgagt ggcctgattg agatctactc gaaaaccatc gccgatatga
    10441 actacgacga ttgtcagggg catccgatgt ggtttgagaa aatcgaacgc tcccacggtg
    10501 ggcccggctc gcagaagtat ccgttgcatc tgcaatctgt gcatccggat ttccgacttc
    10561 actcgcagtt atgtgagtcg gaaactctgc gtcagcaata tacggtagcg ggtaaagagc
    10621 cagtgttcat taacccgcag gatgccagcg cgcgcggtat tcgtaacggt gatgtggtac
    10681 gcgtctttaa cgctcgcggt caggtgttgg caggggcagt agtttctgac cgctatgcac
    10741 ccggcgtggc acgaattcac gaaggggcat ggtacgatcc agataaaggc ggcgagcctg
    10801 gtgcactgtg caaatacggt aaccccaacg tgctgaccat cgacatcggt acttcgcagc
    10861 tggcgcaggc gaccagtgcg cacactacgc tggtggaaat tgagaagtac aatggagcag
    10921 tggagcaggt gacggcgttt aacggccctg tggagatggt ggcgcagtgc gaatatgttc
    10981 ccgcgtcgca ggtgaaatca tgaccacgct gacagcacaa cagattgcct gtgtttacgc
    11041 ctggctggcg cagttgttct cccgtgaact ggacgatgaa caactgacgc aaatcgccag
    11101 tgcgcagatg gctgaatggt tttcgttgct gaaaagcgaa ccgccgctgg ctgcggcggt
    11161 gaatgagctg gaaaactgta ttgccacgct gacagtacgt gacgatgccc gtctggaact
    11221 ggccgctgac ttttgcggcc tgtttctgat gaccgacaaa caagcggcgc tgccgtatgc
    11281 atcggcctac aaacaggacg agcaagagat taaacgcttg ttagttgagg cagggatgga
    11341 aaccagcggc aatttcaacg aaccggcaga tcatctggcg atctatctcg aattgctcag
    11401 ccatctgcat ttttcgctgg gagaggggac cgttcctgcg cgaagaatcg acagtttgcg
    11461 gcaaaaaaca ctgacggcgc tgtggcaatg gttaccagag tttgttgtgc gttgtcgtca
    11521 gtatgacagc tttggttttt acgcggcact aagccagtta ttgctggtgt tagtggagag
    11581 cgaccaccaa aacagataac gtcgtttgtg cgcctgaaaa gacgcgttta gcgtcgcatc
    11641 aggcattatg gggcagttgc cggatgcgtc atcacatccg gcaaacacac atcacggatg
    11701 agctacaaac cgggaaagtc gctggcgcag caggcggttt tcctgcttca ggtgcgcaat
    11761 atcatccatt aacgtcagcg ccaccgcgat cccaggccag tccagagcca gttcatgacg
    11821 caggcgtact gcgcgttgca ccacgatggc ggcatggtcg tcaaataccc aggttgtttc
    11881 ctggatctca cgcggttcaa ccacccccaa accgacaatt tcattcaact cctcttcaga
    11941 gatgccggta tgcaggcaaa attcggtaat agtaaaagtc accgtaacat tagccattat
    12001 gctttccccc aatctttacg tggatcaaaa gacgactggg cgtctgccag ttgctgccac
    12061 agcgcggcag tgttttcatc cggtttcggc ggcatcacga ttttcagtac cgcatacaga
    12121 tcgccggtct gttttttgct caccagacct ttgcctttaa cgcgcaatcg ttgcccggcc
    12181 tggctgcctg gcgggatagt cagcaaaatg ctttctttca gtgttggaac ggtgacttta
    12241 gcacccagcg ccgcttccca cgggctaacc ggcactacaa tttccagatc atggccgaca
    12301 atatcgaaca gcggatgtgg cgcaatatga atcaccagcc acaaatcgcc atttggaccg
    12361 ccgttttcgc ccggcgtccc ctggcctttc agacggatgc gttgaccatt gccgacgccc
    12421 gccgggatct tgacattcag cgttttcgga atttcctgtt cgatcatgcc aaaggcgtta
    12481 taaaccggca ggttatagct gatggtacgc ttatgctcag taagcgtttc ttcgaggaat
    12541 accgccactt cgatttcaat atcgtggccg cgtgtggcgg ggcgttgacg gctctggcgg
    12601 gcatgctgac cgaaaattga cgagaagata tcgtcaaaat cttcggcgtt aaaactctga
    12661 ccgtcgctat gatggaactg acggttaaat tgcggatcgt tgcgatgttg ccacatctga
    12721 tcatactcag cgcgacgttg ttcatcactt aacacttccc aggcttcagc gacctctttg
    12781 aagcgggctt cggcatccgg ttctttgctg acatcaggat ggtatttgcg ggccagtcga
    12841 cgataggcgg tcttgattgt cttgagatcg tccgtcggtt tcacgcccat gatggcgtaa
    12901 taatccttta attccatagc gttatctcgc gtaaatcaac acaaattgaa ggaacccctg
    12961 taaggtaact cctataagtg tagggtaatc ctcaaaattt catatgccaa cacagaatat
    13021 gttattgaaa tcatcgcgga gaggaggtcg cctcaaggtg gggtgctgaa catattttaa
    13081 acaggtgaaa aagggtgagc gatttttgat agtggaacca ggcactttaa gtttaactgg
    13141 ggcgtcatta tttattaaat tttataggcg ctatatatga gtagtaatat acatggaatt
    13201 agttgcactg caaataatta tttgaaacaa gcctggaaca atataaaaaa tgagcacgaa
    13261 aaaaatcaaa aatattcaat cacgcttttt gaaaacacac tggtgtgttt tatgcggttg
    13321 tataaagaaa tcagacgtca aaaagctgaa gactacattc catgcctgga atgcgattca
    13381 ctagaaaaag aatttgagga aatgcagaat gataatgatt tatcattatt tttgagaaca
    13441 ttgcgtacta atgatacaga aacttattca ggggtttcag aaggtattac atatactata
    13501 caatatgttc gagatattga tattgttaga gtgtccttgc cgggcagagg ttcagagtct
    13561 atcacagatt ttaaaggtta ttattggtat ggctttatgg aatatattga aaacattaat
    13621 gcgtgtgatg atgttttttc tgagtattgt ttagatgatg aaaatatgag tatccagcca
    13681 gaatggataa atacgccggg aatatctgat ttagatact
//
LOCUS       DAFNBJ010000130        13198 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000130 DAFNBJ010000000
VERSION     DAFNBJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.130"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            245..4705
                     /gene="gltB"
                     /locus_tag="L2X49_003641"
     CDS             245..4705
                     /gene="gltB"
                     /locus_tag="L2X49_003641"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBN4798662.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4718..6136
                     /gene="gltD"
                     /locus_tag="L2X49_003642"
     CDS             4718..6136
                     /gene="gltD"
                     /locus_tag="L2X49_003642"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBN4798663.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6320..7447
                     /locus_tag="L2X49_003643"
     CDS             6320..7447
                     /locus_tag="L2X49_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBN4798664.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHTPTETHILPQQAIRDPFVLV
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(7507..7971)
                     /locus_tag="L2X49_003644"
     CDS             complement(7507..7971)
                     /locus_tag="L2X49_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBN4798665.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(7968..8843)
                     /gene="nanK"
                     /locus_tag="L2X49_003645"
     CDS             complement(7968..8843)
                     /gene="nanK"
                     /locus_tag="L2X49_003645"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBN4798666.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(8840..9529)
                     /locus_tag="L2X49_003646"
     CDS             complement(8840..9529)
                     /locus_tag="L2X49_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBN4798667.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9577..11067)
                     /gene="nanT"
                     /locus_tag="L2X49_003647"
     CDS             complement(9577..11067)
                     /gene="nanT"
                     /locus_tag="L2X49_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBN4798668.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(11165..12058)
                     /gene="nanA"
                     /locus_tag="L2X49_003648"
     CDS             complement(11165..12058)
                     /gene="nanA"
                     /locus_tag="L2X49_003648"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBN4798669.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(12180..12971)
                     /gene="nanR"
                     /locus_tag="L2X49_003649"
     CDS             complement(12180..12971)
                     /gene="nanR"
                     /locus_tag="L2X49_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBN4798670.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         3149 a         3632 c         3544 g         2873 t
ORIGIN      
        1 ctgtatcagc tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg
       61 ctcatcatat ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag
      121 gacaacaagg gggcgaatgc gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct
      181 ttctgtgccc gtgcgcagcg gttcggaagt ggggttcccg cagagcctgg gggaggttca
      241 cgatatgttg tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca
      301 catagaaggc gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat
      361 gcagcaccgt ggcgcgattc tcgccgatgg taaaaccggc gacggttgcg gcttgctgtt
      421 acaaaaaccg gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa
      481 aaactacgct gtcgggatgc tcttcctgaa taaagatcct gaactcgccg ctgccgcacg
      541 ccgcatcgtt gaagaagagc tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt
      601 ccccactaac gaaggcgtgc tgggtgaaat cgccctctcc tctctgcccc acattgagca
      661 aattttcgtt aacgctccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat
      721 cgcccgccgc cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct
      781 gtcgaatctg gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta
      841 tctggatctt gcggacctgc gtctggaatc ggccatctgc ctgttccacc agcgcttctc
      901 cactaacacc gtaccgcgct ggccgctggc gcagccgttc cgctacctgg cgcataacgg
      961 tgaaatcaac accatcaccg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca
     1021 gacgccgctg atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggttctga
     1081 ctccagttcg atggataaca tgctggaact gctgctggca ggcgggatgg atatcatccg
     1141 cgccatgcgt ctattagtac cacccgcctg gcagaacaac ccggatatgg acccggaact
     1201 gcgtgccttc tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat
     1261 cgtgatgtcc gatggtcgct tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc
     1321 acgctacgtc attaccaaag acaagctcat cacctgcgcc tctgaagtcg gtatctggga
     1381 ttatcagcct gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggtgat
     1441 cgacacccgc agtgggcgta ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg
     1501 ccatccatat aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct
     1561 gcccgatgaa gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa
     1621 acagtttaac tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg
     1681 tcaggaagcg gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc
     1741 gcgcattatt tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga
     1801 cccgctgcgt gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt
     1861 cttttgcgaa gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta
     1921 ctccgatttc aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga
     1981 tatcaccttt gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa
     2041 agccgaaaaa atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc
     2101 taaagatcgc ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt
     2161 cgatcaaagc ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc
     2221 gcaccacttc gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta
     2281 tgaaacgctg ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat
     2341 gctcaactac cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat
     2401 ctccaccatc gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tgcacgatga
     2461 tgtagtgggc ctgtgcttcc agggggcggt cagccgcatt ggtggggcga gctttgaaga
     2521 cttccagcag gatctgctga atctgtcgaa acgtgcctgg ctggcgcgca agcccatcag
     2581 ccagggcggc ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt
     2641 ggtgcgcacg ctgcaacagg cggtacagag cggcgagtat agcgactatc aggaatacgc
     2701 gaagctggtt aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg
     2761 tgaaaacgcg gtcaacattg ctgatgttga accggcaagc gaactgttta aacgctttga
     2821 taccgccgcg atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc
     2881 gatgaatagc atcggcggta actcgaactc cggtgaaggc ggcgaagacc cggcgcgcta
     2941 tggcactaac aaagtgtcgc gcatcaagca ggtggcttct ggtcgcttcg gcgttactcc
     3001 ggcgtatctg gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg
     3061 cgaaggcggt cagttgccgg gcgacaaagt cactccttac atcgccaaac tgcgctattc
     3121 ggtgcccgga gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga
     3181 cttagcgcag ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa
     3241 gctggtttcc gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga
     3301 tttaatcacc atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa
     3361 atacgcaggt tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa
     3421 tggtctgcgt cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat
     3481 catcaaagcg gcgattctcg gcgcagaaag cttcggcttc ggcactggcc cgatggtggc
     3541 gctcggctgt aaatatctac gtatttgcca tctgaacaac tgcgcaacgg gtgtagcaac
     3601 tcaggatgac aaactgcgta agaaccacta tcacggcctg ccgttcaagg ttaccaatta
     3661 ctttgaattt attgcccgtg aaacccgcga gctgatggca cagctgggcg taacgcgtct
     3721 ggtggatctg attggtcgca ccgacctgct gaaagaactg gacggtttca ccgccaaaca
     3781 gcaaaaactg gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact
     3841 ctactgcacc gaaaacaacc caccgtttga taacggcctg ctgaacgcgc agttgctgca
     3901 acaggcgaaa ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atattcgcaa
     3961 caccgaccgt tctgtcggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca
     4021 ggggctggca gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg
     4081 tgtgtggaac gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg
     4141 taaaggcatg gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag
     4201 ccatgaagca agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta
     4261 tgccgcaggc cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt
     4321 agaaggcatt ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg
     4381 taaaaccggc gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga
     4441 aagcggtgat ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga
     4501 tctggcgatc catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg
     4561 ctctcagcgc ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct
     4621 ggttaaaccg aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc
     4681 agagttgcgc gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt
     4741 tatcgacctg cagcgcgttg atccgccaaa gaagccgctg aagatccgca aaattgagtt
     4801 tgttgaaatt tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct
     4861 gtcgtgcggc aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg
     4921 gctgaagctc gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa
     4981 caccctgccg gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg
     5041 cactctgaac gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga
     5101 taaagcgttc gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa
     5161 agtggcgatt atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa
     5221 cggcgtaaaa gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg
     5281 tattccggcc ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg
     5341 catgggtatt gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct
     5401 gctgagtgat tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg
     5461 gctggaaaac gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac
     5521 caaacagtta atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa
     5581 acgcgtggtg gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg
     5641 ccagggagcg aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc
     5701 ccgccgcgaa gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc
     5761 actggggatt gaagtgaacg gtaacggtaa agtcagcggc gtaaaaatgg tacgtaccga
     5821 aatgggcgaa ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca
     5881 tatcgttccg gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg
     5941 gctggcaaaa cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag
     6001 cgacaacgcc ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg
     6061 ttccgatctg gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa
     6121 ctggctggaa gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa
     6181 attctccttt ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct
     6241 catctggatt gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg
     6301 ccatataaag ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca
     6361 aatccacgac ggtattattc atctggtcga tagcgcccgg acggaaacgg tacgtagcgt
     6421 taacgcgctg atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca
     6481 aggtggcgag gccagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt
     6541 aagtctaagg tataagcgtg ggttctctgc aaaaaactta cgccaaatga ggctttttta
     6601 cctctttttt caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg
     6661 aattccgcag acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc
     6721 caaatctttc cccctgccct ggtccaccta cgtccgttta ctttccgtta aaaacgctga
     6781 cgcccgcagc ttttatgaaa aagagacgct ccgctgtggc tggtctgttc gccagctaga
     6841 acggcaaatt gcgacccaat tttatgagcg aacactactg tcacatgaca aatcagccat
     6901 gctgcaacaa cacactccta ccgagacgca tattcttccg caacaggcga tacgcgatcc
     6961 ctttgtgctc gtatttctgg aattgaaaga tgaatattca gaatctgatt tcgaggaggc
     7021 gctgatcaac cacctgatgg atttcatgct ggaacttggg gatgattttg cctttgttgg
     7081 tcggcagcga aggttacgca ttgatgacaa ctggtttcgt gtcgatctgc tgtttttcca
     7141 ccgccgttta cgctgcctac taatcgtcga tctaaaagtg ggcaaattca gctatagcga
     7201 tgccggacag atgaatatgt atctcaacta cgccaaagag cactggacgc taccggatga
     7261 aaatccgccc atcggtctgg ttctctgtgc agagaaagga gccggagaag cacattatgc
     7321 tctggcaggt ttgcctaaca ccgttctggc aagcgaatat aagatgcaac tacctgatga
     7381 gaaacgactc gcagatgaac tcgttcgaac acaggcggtg ctagaggaag gctatagacg
     7441 ccgttaattt cagcggtttc gaattctgca gacagtgtct gcagaatctt taaaacgaac
     7501 taagttttaa gccattaaat cagccttaac cttcaccaca acctttttaa tctctccagg
     7561 ctcgccgaca acgcatcctg gtttatgcgg ttcacctggc ataaacacgg cgaacattcc
     7621 cggttttaag atgatggctt gctcgttatc aatggtgctg caaagctgat aatcatcctc
     7681 atggtggaac tcttcacact ggcgcgcagt gcctgccatg ccaaacagaa tccgttcctc
     7741 accgtttaat aacagttgga tatcaatgta ttgctcgtgc aattccgctt ttttctcgac
     7801 gggcgattga gtgttaaacg tcatgacatt cataaagata ttgtcgccct gtaattcgta
     7861 acgacccggc gctttttctt gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa
     7921 cgcaggatgt aacccagcag acggtaatga ctgtacttca cccatcatca taatttttct
     7981 ccctgggcca acagcgcagc cccaagtaaa cctgcatcgt ggcggtagtg cgccgccagt
     8041 aaatcaacat gaaatgccgc tggctcctgc gccagatacg tttccaccag cgccagatac
     8101 ccttctgcca gcccaacgct gccaccgacc accacgcact ggcaatcagt tgtggcttta
     8161 atatcagcga tcagccttgc aagcgtacgt gcggagcggt gaatcagctg ctgcgcctgc
     8221 tcgtcgccct gcccggcgcg tgtgaaaata gttctcgcat ccgcgcccgc cagttcaccc
     8281 tgcgctgccg ccgcaatgcc gcgaccagaa gcaatcgcct ccacgcaacc tgtgcgtcca
     8341 cagccacaga ccgggccgtg tggatcggca agcgtatgcc cgatatgccc cgccagaccg
     8401 ccagggccgg taagcagttt gccgccgctc actacaccgc cgccgacgcc agtggaaacg
     8461 gtgataaaca ccatctcggt tacatcgcct tccagcgcct gatactccgc ccatgctgca
     8521 gcctgcgcgt cgttaatggc aatggtcggc aaattggtaa gttgttccag cgttttgact
     8581 aacggaaagt gtagcaatcc accgagatta tgcggattca gcgccagcaa actgccgtcg
     8641 cgaataattc cggttgaagc gatggcaacc cgctgcgcat gagcttgcaa cggagagact
     8701 aatgcggata aggcatcacg caaggcttct ggtgtctggc tggctggcgt aggaagttca
     8761 cgacgatcgc ggatctgccc gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg
     8821 atatcaatcg ccagcgtggt catagcaccg cctttttcat cgctgtgttg taccactgac
     8881 aaatgtgttc aagacgcgta atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca
     8941 tcgcatctgc cgcctgagca ggcgtgttgt aacgcccttc ggcaatcacc ctacatccgg
     9001 cgtcgctcaa cgttttcacc agcgccagat ccggctcttc tggcgtttca ggcgtggtat
     9061 agccagaaag cgtagtgcca ataatttcgg ctcccagctt ttggcatgcc aggccgtctt
     9121 ccggcgttga gcagtcggtc atcgccagta aaccgtgatg gtgaatacgt gccagcagcg
     9181 tttcaacagg caccggacgc gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg
     9241 cctgcgccag cgcatcaaca tcttcaatat aggccgtgat gcgtaccgga gaatcctcca
     9301 gatcgcgttt cacaattcca ataatcggca cgctcaccac cgcacgcgtg gcttgcagat
     9361 ttgccacacc ttcaatgcga atggcaaccg cgcccgcctg ttctgccgct aatgccatgg
     9421 cggcgacgat ttcgggttta tcgagcgggc tgtccggaac cggctggcag gagacaatca
     9481 ggccaccgtt agcagcgatt ttttgatcca gttgtgcaag taacgacata catcttccct
     9541 tagcgaaagg cccggtacat agaccgggca acaggattaa cttttggttt tgactaaatc
     9601 gtttttggcg ctgccaaatg gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg
     9661 agtacgcaat gcttccgggc gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat
     9721 cagcaggatc accacaaatg tcagactgaa cgatagcgat gccagcgcag tacccagatc
     9781 cagacgttga gcgatcaacg cgccgatgat tggggccagt gcgccgccca atgcgccaac
     9841 gttgtaggta aagcccaggc ccgctgcacg ctggtcggta tcgaaataac cgccaatcag
     9901 tttcggtaag atcccggcga tcccttgtcc aagcatttgc tggaagaaca gtaacagacc
     9961 gagcacccag acgtttgcgc cgccaatcgc aaataccgga ataatcaaca gctgcgaggc
    10021 caacaggcta caaacgtagg ctttgcgggt tcccagccag tcaccgagga agccgccgac
    10081 acagcacccc accgcagccc caaagccgct aaagaacagc acattggcga cagtatgcgg
    10141 gtcataagcc agatcggttt tcagatacgt tggcagcagc gcctgaatcg gccatgagta
    10201 gaggaaagca aacaacacga ccaccatcag cattacgccc gttggccagc gtttgcccgt
    10261 actctgcacc ataaagctga taaagattgc ggcgcataac agcccaagaa cagcgacgat
    10321 cgcggcattt tgcaggttac cggcgaagca gaaccacagc gcagtagccg ccgccagtgt
    10381 cattacgata ttggcaatgc gatgttcacc acggtagaga atatccacca ttgtgcgtac
    10441 cggtgcttta cccccgtgtt tctctttcca gtcttccgct tccgggatgt ttttacgcag
    10501 ccagagagca aagatgattg gcaaaatgcc gataaagaac agcgcacgcc agccccagac
    10561 cggaaccacc aggctataga cctgagcggc aacgacggcc cccacagaga agcctgaaat
    10621 cagaaaacca ctggctttgt tacgcagatg ttttggccag ctttcaatga cataggtggc
    10681 gctggaaccg tattcacccg ccatccccat gccgatgacc agacgcgcga taaacatggt
    10741 gatgtagcct ggcgcaaagc cgcaggccag cgtcccggcc gagaagagaa cgatgctggt
    10801 gaccattgcc agacgacgcc cgtagcggtc acccatagcg ccgagcatca ggccgccgaa
    10861 ccagcgagag ataaaggctg cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa
    10921 ttcaccctgt acttcagtga gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag
    10981 atatcccaac caggcagcgg aaaatgcgcg ccattgtgca cggttgagat ggcgatacca
    11041 cgggatattc tgggttgtag tactcattgt gagtctcccg cggtgggcga tgcccacacg
    11101 aaattgtagg gtacagatgc gtttatttcc cctcacccgg taggggcgag cgaggggaaa
    11161 caactcaccc gcgctcttgc atcaactgct gggccagcgc cttcagttct ggcagatatt
    11221 tttcatctac cggtccaaac ggtttgcggc acagcggcac agaaacgaca tccatataat
    11281 ggaggacagt tttcaggccg cggaatacgc ccgttttgat cagtaaatca atgactttat
    11341 tgcattcagt ttgcagtttc tgcgcggtct ggatatcgcc ttctttcagc gccttaacga
    11401 tcccctgata gcgccagccc atgatgttgt aggtactacc gataccacca tcagcgcccg
    11461 ccagcagacc agaggcgaag atttcgtcgt aaccgttata gagcacaaga tcaggatgtt
    11521 cacgacggat ctgctccatc tgatagagat cgccagaggt ctgtttcagc gcacctacgc
    11581 caggcaatgt aacaagtgtg ttgatctgat ccagggtcag ttttacccca ctcagggctg
    11641 gaatgttgta caccaccatc ggcaaaccat ccgccgaatc aataattgcc cgatagtgat
    11701 cgcagtgttc ttcaaagctg aaaggatagt agaacggcgt gacggcggag acggcatcga
    11761 agccataacg tttagccgat gccgcaagtt gttggctttc ggcggtgctg acgcaaccga
    11821 cgtgggcgat gagtttaatc ttacctttcg cctcttcggc gacgatttcc agtacctgtt
    11881 cacgctcgga aaggctttgt acaaaggcct cgccggtcga accacccacg tataaaccgt
    11941 cgatgccctg ctgaatattg aactgaacca ggcgacgcag actcgcttta tccagtgctt
    12001 gttgttgatc aaaaggagtc aggagtgcag ccattacgcc acgtaaattc gttgccataa
    12061 atacctctga agtgatgctt gtctgataaa cgatatacct ttatacctgt tataccagat
    12121 caattaagca acaccccata cagaaagctt ataatgcgat ctgcttcact aaagtggcat
    12181 tatttctttt tgttggtggt ctgaccgaaa gcgtgccagg tagcagagac gctgttgaga
    12241 tgcgattgca acgcacgatc ggcttcgtca ggatcatgac ggcggatcgc atcaacgatc
    12301 gcaatatgct gttgataact aacgttgtta tgttcgtgca gtgcctgatc ggtaaccgtt
    12361 gggcgtgcgg caataagcca gtcgagcagg gcaacgtgga tcgccatgaa gattgggtta
    12421 ccggggatct ccgccagcac gcggtggaaa tcaacgtctg aacgaatgaa tgccgcgttg
    12481 ttatccagcg actgactgtt aatttccagt gcttttgcca gcaaatcgat ttgctcatcg
    12541 gtggcatgtt cagccgcata gcgcaccagg ctggattcaa agaacagacg taattgttcg
    12601 aaatgggcaa tcccaccagg atgagaaagg aaatctttcg ccatgccgga aagctcgccg
    12661 atgatggtgt ccgcagaagg acgtgagacg cgggcacgtt cgccgttgtt gatttgcacc
    12721 agacctttgc gttttaacgc tgccagcgct tcgcgcacag aaggacgccc gacgttaaag
    12781 aacgccatca gttcgcgttc agacggtaat tgttcacctt cgccaaattc acgacggcgg
    12841 atcatctgtt ccagctcttc ttccaccatt tcggagagtt ttttacgcgc cagcgggcgg
    12901 ctacgcaagt tgcgaccaat tgcaggtgaa gaatcttcgg tttgcgaatc aaatgcgttc
    12961 ataaggccca ttctgtaagg tcagtgtgat taacatcatc agtgacatcc tatcacagga
    13021 ttgaaagtag gggaaaatgg caggtttttc tctttgtgcc tcatcattac cataattaac
    13081 ggaataatta actattgcga aaaattaatg taacgcagat aaaaacatcc cgtttgaatt
    13141 atttataaga ctattcacga gcattatgaa tattatgaat gtgttcttac aaaataat
//
LOCUS       DAFNBJ010000131        13168 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000131 DAFNBJ010000000
VERSION     DAFNBJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.131"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            39..1946
                     /gene="yegI"
                     /locus_tag="L2X49_003650"
     CDS             39..1946
                     /gene="yegI"
                     /locus_tag="L2X49_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HBN4798671.1"
                     /translation="MTTLGLELGKGGEGAVYDINEFVDSVAKIYHTPPPALKQDKLAF
                     MAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYSPAHRRQRYPHC
                     AWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDSDSFQINANGTL
                     HLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGRHPYSGVPLISD
                     AGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQAFTESGVATGR
                     PTAKAWVAALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIYFIDLGEEVITT
                     GGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYIILLEIALSALS
                     LLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRAKMDYDHLVRQI
                     QQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQKFLEGFFIDVA
                     SIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKASCERRFVFRP
                     NEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMPLMEPLRQAAEK
                     LAQAQADLSRC"
     gene            complement(1958..3310)
                     /gene="yegD"
                     /locus_tag="L2X49_003651"
     CDS             complement(1958..3310)
                     /gene="yegD"
                     /locus_tag="L2X49_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBN4798672.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            3444..4301
                     /gene="alkA"
                     /locus_tag="L2X49_003652"
     CDS             3444..4301
                     /gene="alkA"
                     /locus_tag="L2X49_003652"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBN4798673.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            complement(4340..7657)
                     /locus_tag="L2X49_003653"
     CDS             complement(4340..7657)
                     /locus_tag="L2X49_003653"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBN4798674.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWNCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAVSVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            7975..8616
                     /gene="udk"
                     /locus_tag="L2X49_003654"
     CDS             7975..8616
                     /gene="udk"
                     /locus_tag="L2X49_003654"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBN4798675.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            8708..9289
                     /gene="dcd"
                     /locus_tag="L2X49_003655"
     CDS             8708..9289
                     /gene="dcd"
                     /locus_tag="L2X49_003655"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBN4798676.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            9311..11164
                     /gene="asmA"
                     /locus_tag="L2X49_003656"
     CDS             9311..11164
                     /gene="asmA"
                     /locus_tag="L2X49_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBN4798677.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11438..13021)
                     /locus_tag="L2X49_003657"
     CDS             complement(11438..13021)
                     /locus_tag="L2X49_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBN4798678.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
BASE COUNT         3190 a         3430 c         3478 g         3070 t
ORIGIN      
        1 tgaagacaaa tataaaagta tttacatcaa caggtgaaat gaccactctc ggtcttgaac
       61 tgggcaaagg cggtgaaggc gcggtttatg atatcaatga gtttgtcgat agcgtcgcca
      121 agatttatca cacgccgcca cccgccttaa aacaggacaa acttgccttt atggctgcga
      181 cagctgacgc gcaattgttg aattatgtcg cctggccgca ggcaacgctt cacggtggac
      241 gaggcggaaa agttatcggt tttatgatgc caaaagtttc tggtaaagaa ccgattcata
      301 tgatctatag cccggcacat cgtcgtcaac gttaccctca ttgtgcgtgg gattttctac
      361 tctatgttgc gcgcaatatt gcttcatctt ttgctacggt tcacgagcac gggcacgtcg
      421 tgggtgacgt aaaccagaac agttttatgg taggtcgcga cagcaaagtg gtactgattg
      481 atagcgactc ctttcagatt aacgccaatg gcacactgca tttatgcgaa gtcggcgtgt
      541 cgcattttac gccgccagag ctacaaacct tgtcgtcatt tgtcggcttt gaacgcacag
      601 aaaatcacga taattttggc cttgcgttgc tgatttttca cgtcttgttt ggtggccggc
      661 atccttattc tggtgtgccg cttatctctg atgcgggtaa tgcgctggag acggatatta
      721 cccatttccg ttatgcctac gcgtcagata atcagcgacg tggtttaaaa ccgccgccac
      781 gatcgattcc gctatcgatg ttaccgagtg atgttgaagc catgtttcag caggcattca
      841 cggaaagtgg cgtggcaacc gggcgtccga cggctaaagc gtgggtagcg gcacttgatt
      901 ctctgcgcca acagttaaag aaatgtaccg tttcggcaat gcatgtttat cccggtcatt
      961 tggctgactg cccatggtgt gcgctggata atcaaggtgt tatctatttt attgatctcg
     1021 gcgaagaggt cattaccacc ggcggggatt ttgtgctggc aaaagtctgg gcgatggtga
     1081 tggcgtcagt agcaccgcca gcattgcaac tgccattacc cgatcatttc caaccgactg
     1141 gcaggccgct tcctttaggc ctgttacggc gcgaatacat cattctgctt gagatcgcac
     1201 tgtcagcatt atcgctgctg ctttgcggcc ttcaggcaga accgcgttat attattttgg
     1261 ttcctgtgct ggcggctatc tggattattg gcagtctgac aagcaaagcg tacaaagcag
     1321 aagttcagca acgccgtgag gcatttaatc gtgcgaaaat ggactatgac catttagtcc
     1381 gccagatcca gcaggtaggc ggcctggaag gttttatcgc caaacggacg atgctcgaaa
     1441 aaatgaagga cgaaattctc gggttaccgg aagaagagaa acgcgctctg gcagcacttc
     1501 atgacactgc aagggaacgg cagaagcaga agtttctgga gggatttttt attgatgttg
     1561 cctctattcc cggcgttggc cctgcgcgta aagcggcgtt acggtctttt ggtattgaaa
     1621 cagccgcaga tgttacccgt cgtggcgtta agcaagttaa agggtttggc gaccatctga
     1681 cccaggcggt tatcgactgg aaagcgagct gtgaacgccg ttttgtgttc aggccgaatg
     1741 aagcgataac gccagcagac agacaagcgg taatggcgaa aatgaccgcc aaacgacatc
     1801 ggctggaatc gacgttgact gtcggcgcga cagagttgca gcgattccgc cttcatgctc
     1861 cagcacggac catgccgttg atggaacctt tacgtcaggc ggcagaaaaa ctggctcagg
     1921 cgcaggcaga tttaagccgc tgctgagcca ttttctttta acgaaacacc acttccgccc
     1981 agcgtgccag cccggcggtg acggagccaa agtcatcgcc gcctgcaatc ggaattcccg
     2041 gcaactgttc tgccagcgct tttttaatca gcggcgaacg ggcgctaccg ccggtcaggt
     2101 agataacgtc cggtttttcc tgggcgttat ccagggccag ttgcacctgt tccagaatcc
     2161 gcgccagtgg ctggctgagg gcgctttcca gcccttgctg gctaatcagc gtagccagtt
     2221 catcgctgat aaacggcagt gaggcgcggg tttccgctac gcttgaaaga gcaattttgc
     2281 actcttctgc gctacgcacc aggcgatagc ttaaacgctg acgccagact ttctgtagca
     2341 gggccacttt ttccggttcg cgggcatcgc gtaccagatc gttaagcaga cgaccgttgg
     2401 cactactgta gaaatcactc tgcgcaggta cgtcgttgat ggcaaccgca ttccaccacg
     2461 gcagaatcgg cagggcgatg cctttttcgg tttcgccacc catgcccagt aacggcatca
     2521 ggtttttaaa cgccagtgcg atgtccagat cgttaccgcc aatacggcaa ccactgtgac
     2581 ccagcaggct ggcttcacga tcgagacgcg aacgccactg cggccccatc agcagcaatg
     2641 aacagtcagt cgtaccaccg ccgatatcca ccaccagcac ccgtttttct tcctgcaagg
     2701 tggcttcgta atccagccca gccgcgaccg gctcgtactg gaataccacg tccctgaatc
     2761 cggcacgctt cgccgcgcgt tccagaatcc cttgcgcctg ggcgtttgct tcatcgccgc
     2821 ccagcccctg gaagttgatc gggcgaccaa tcaccgcctg agtaatcgct tctggcagct
     2881 gcgcctgcgc ctgctggcga atgtgcaaca tcattgcgca gaccagatcc tcaaacagcg
     2941 ctacctgctg cggttttaag ccgctggcac cgaggaacga ttttggtgat ttcacaaacc
     3001 acacttcttc tggatcatca atgtactgtg ccagtgagga aagaccgaac tgcacgcttt
     3061 tcgccgtaac atcgatatct tcttcgcggt tataacgaat cgcccgacgt aacagcgcct
     3121 gcgtttcatc gtcgtctgcc ggaacatcat gatggcggta cagccattca cttactgctt
     3181 cacgcgttgg cgcgcaaagc attgaaggca gcagcgtgct gtcgttttcc attttcagta
     3241 attgcggttt accgtcacgc atgaccgcca ctgaacagtt tgctgtaccg taatcaaaac
     3301 caataaacac gaattaatcc ccatgccggt gaagaagggg cgtgacttta gcgaaatgtt
     3361 gccgtcgcga caaccggaat atgaaagcaa agcgtagcgt ctgaataacg tttatgctga
     3421 aagcggatga ataaggagat gcgatgtata ccctgaactg gcagccgccg tatgactggt
     3481 cgtggatgtt gggatttctc gccgcccgtg cggtgagtgg tgtggaaacg gtcgctgagg
     3541 attattatgc ccgtagtctg gcggtgggcg aatatcgcgg cgtggtgact gctattccgg
     3601 atatagcccg ccatactctg cacataaatt taagtgcagg tttagaaccc gttgccgctg
     3661 agtgtctggc gaaaatgagc cgcctgtttg atctgcaatg taacccgcag attgttaacg
     3721 gtgcgttggg caagttaggc gcggcgcggc ccggattgcg tttacccggc tgtattgatg
     3781 cttttgagca gggtgtgcgg gcgattttag gccaactggt gagcgtggcg atggcggcaa
     3841 aattgactgc caaagttgta cagctttatg gcgaacggct ggatgatttt ccggagtata
     3901 tctgcttccc cacgcctcag cggctggcag cagccgaccc gcaggcatta aaagcgttag
     3961 gcatgccgtt gaaacgggca gaggcactga ttcatctggc aaatgcggcg ctggagggca
     4021 gcttaccaat gacaattccg ggcgatgtgg agcaggcgat gaaaacgctg caaacttttc
     4081 cgggtatcgg gcgctggacg gcgaattatt tcgctttgcg tggctggcag gcgaaagatg
     4141 tttttctgcc ggatgattat ctgattaaac agcgatttcc ggggatgaca ccggcacaaa
     4201 tccgccgtta tgccgagcgc tggaagccct ggcgttctta tgcgctgttg catatctggt
     4261 atacggaagg ctggcaacca gacggaacgg acgaattgta ggcctgatac gtcgcatcag
     4321 gcaatgggca aacgctccct cagttaatcg cgaaataact gctattcacc agcaaatcca
     4381 gcggttgggc atcggcaatc acatcaccat aaatcagatc gacgccgatg ccagaaagcg
     4441 tatccatcac taagggtaaa acgactggcc cggcgatggt tttcatcccg agtcgctgag
     4501 cgtgcccctg aataatcgta atcagcatct catccatcaa attaccctgc acgttggcgc
     4561 ataactcacc atcaagtagc aggtaatctg ccatattcgc tttcagcgag ttgaagattt
     4621 gcagatcgcg gcccacctgg ctgagcacta tccgacatcc cgccagccgc agtttttgca
     4681 cgcttactgc gtgatctaaa atcgcttcag ccggaataat cagatgtaat aaccgtggtg
     4741 gtagagggct attttccagc tgctcaagca gatcattcac cagcgtggcg ctactcaaac
     4801 cggcaacgga aagagggagg gcgatgctta tgcctttact ggcaaccgct ttggcggcct
     4861 gctggaaaaa atcgtggaat acccgtcggt caagagcatg gctaagtgcc ggatcgctga
     4921 agctacgacg aaatgtttgt tcatcaataa tctcgccttc gcaattccag agcttaagtg
     4981 aaatcagcca caaattacgt gcttccggga tccgtggcga agcgacaccg tgggcgatca
     5041 tcatcaactg attctcttta atcatccgcc actgttcatc aagcgacatc gctgcccgct
     5101 cgctatgtgc ggcagcttgc tgcggttcgt aaatcgtcac ccggccccgg ccaccatttt
     5161 tggaggcata acaggcgata tcagcctgcg acatcacttc agccgcctga tgattgttgt
     5221 catcaatcaa ggtaatcccg gcactggcac ctacccgatg tacacgtcct tcccagataa
     5281 agtgatagtc attcacggca ctgataatgc gtgtagcgat aaaacgcgcg ctttcaacat
     5341 tacagtctgg tagcagcaga ccaaattcat ccccaccgag tcgcgccagc acgtcgctgg
     5401 aacgcagcat actcagcatt aacgacgcca gttcgcgcag caaagcgtca cccgccgcat
     5461 gcccggcgct gtcattcacc gctttaaagc gatcaagatc gataaacacc agggcatgtc
     5521 gctgatgtgt actgtttacc gtttgcagca ggatacgcag ttgtttctca aaactggcgc
     5581 gattggcgag atgcgtcagt gcatcatggg aggcgctgta gctcagctgg cgcagcattt
     5641 tgcgtgattc ggtaacgtcc tgaatcacca gaaccgaacc aatattgctg ccgtccagag
     5701 tacttaacgg cgtaatactg taatgcacgt cgtaactgcc gccgctccga cagtgcaaca
     5761 ccacatcttg ttcgatcgcg gaacgtgagg tgtcggcact gtaaatgttc tccattaatg
     5821 gtccgttgtc gccaaaagta atatgcaaca ccgtcaggag cggaacacct aacgcttctt
     5881 cctgcgtcca gccgctcatc ttctccgcca ctggattcat aaaggtaatt ttcatcgcca
     5941 tatcaataca gaccacggct tcaccgatgg aatcaagcgt aatgtgcagg cgctcttttt
     6001 cctgaaacaa tgcctcgtta agctgtttaa cctcggtcat atccatatta atgccgagca
     6061 ggcgttcgac ttcgccttct ttattcagta cccggttggc gagggcgcgg atatggcgaa
     6121 taccgtcttt tacggtaatg cgaaattcca gcttaaaggg cgagcgtgat tgcaacgaat
     6181 cacgaatcac tttttcggca tgctggcgat cttccggcag cacgcactcg taccacacct
     6241 gccagttcgg tttgatatgt ggaggaattt catacagctc gaacatccgc ttatcccagc
     6301 taaaaatatt cggcttcaac tcccactccc agatgccaat cccgcccgct tcgttagcca
     6361 gcgtgatgcg ctccatcagt tgctgattca cctgttcggt gcgttttagc tcgttaatgt
     6421 cttcaatctg agcgataaaa tagagcggcg tgccatccgt gtggcgcacc agtgagacgg
     6481 caagcaacgc ccagacaaca tcgccattgc ggttgtagta gcgtttttcc attgaatagg
     6541 tgtttatttc accgcttatc agcttttcaa cctgttggag atctttattg agatcctccg
     6601 gccaggtcag ttgctgaaag gtgagtccgc gcagctcttc ctgactgtac ccgagaaact
     6661 ggcagagcgc tttgttggtt tgcagccatt gtccctcggt gcccactaac gccataccga
     6721 tagcggaata ttccatggcg ttccgaaaac gggtttcgct ttcggaaatg tgtttgcgtt
     6781 ccgcacgaaa ggcatacatc accatcgtca tgatgttggc gggcagcagg atcagcaaaa
     6841 acggtagcca cggcatatgg ctcatcaggt atgtacgcgg cgtagcaagc agggagggat
     6901 ccgcggccat catcagcgac accatcatca ccgtggtaag gaagatcaaa aaggcttcca
     6961 ttcgtggcag gcgcacggcg ctccacatca acagcacaat aatgaaagta aaaggccacg
     7021 gcagatacag catcgaaagc cagcttaacg tcagtgtgat ggctaacgtg agcagcgact
     7081 caaaaagcaa ccgtgggttg cgatggcgca gcagatagtg tggtttaaat aacaatccca
     7141 gcggcaccag tgccagagcg ccgatggatt ctgacagtac ccatatcaaa aatgccctga
     7201 gaggatcgtc tccgggcgtc agcaggacaa ccagaacacc ccctagcaga ggcggaacaa
     7261 tggcgctgcc gaatgcaaga cgcagccagt cagccagatt ttgcaagggg ttataccacg
     7321 gcagcaattt acgtagcagc actgccccga ccacggcttc aacaatattg atggtcgtcc
     7381 aggtcatgtt cagcgagctg gtggaaaaaa gcaggatgga tgcggcgata tttcccagcg
     7441 agcagctcag cgcaattccc ggccacatgc gcccggcatg gcgataaaac gccaccatca
     7501 tgatggacgt cgggaaccac agtggggcga gttgggtgcc aaactgcgaa agctcaagcg
     7561 agaaaagggt aaagataaac gagactaaac ctaaactgac caggtgaagc agcgggtggg
     7621 gcagggcaat taatacatgc tgtgattgtt tgctcattac cgctttatcc gcaagaccag
     7681 gtcgccagta ttcgtttctg tgtttatgct agtacaaaag ttttacattt tatatgcgaa
     7741 ttgctcataa agtgacgtaa aggcggataa tttgcgcaac tgcgtataac attttttacc
     7801 ttacataaaa ctgatcaacg taatttgcca ccgggaattc tttgtcaaca attaactctg
     7861 agcgcgcggc aacgctattc gactggtatc agacggatga aatccctata attgccgcgt
     7921 ttggcgcttc gtcgccccct tcctaacatc caggttaatc aggtcgctaa atatatgact
     7981 gatcagtctc atcagtgcgt cattatcggt atcgctggcg catcggcttc cggcaagagt
     8041 cttattgcca gtacccttta tcgtgaatta cgtgagcaag tcggtgatga acacatcggc
     8101 gtaattcccg aagactgcta ttacaaagat caaagccatc tgtcgatgga agaacgcgtt
     8161 aagaccaact acgaccatcc cagcgcgatg gatcacagcc tgttgcttga gcatttacaa
     8221 gcgttgaaac gcggctcggc aattgacctg ccggtttaca gctatgttga acatacgcgt
     8281 atgaaagaaa cggtgacggt tgagccgaag aaggtcatca ttctcgaagg cattttgttg
     8341 ctgacggatg cgcgtttgcg tgacgaactt aacttctcca ttttcgttga taccccgctg
     8401 gatatctgcc tgatgcgccg catcaagcgt gacgttaacg agcgtgggcg ttcaatggat
     8461 tccgtgatgg cgcaatatca aaaaaccgtg cgcccgatgt tcctgcaatt cattgagcct
     8521 tctaaacaat atgcggacat tatcgtgccg cgcggcggga aaaaccgcat cgcgatcgat
     8581 atattgaaag cgaaaataag tcagttcttt gaataagctt gataaattgt gtaccgttca
     8641 gtgataacct ggtatgccct tgacgtaatt ggcgttaagg gagtgatgcg cgaaaggaga
     8701 aaatgccatg cgtctgtgtg accgagatat tgaagcctgg cttgatgaag gccgtttgtc
     8761 gatcaaccca cgtccgccag tggagcgtat taacggcgcg acggtggatg tacgcctggg
     8821 caataaattt cgtaccttcc gtggtcacac ggcagcgttt atcgatctga gcggtcccaa
     8881 agatgaagtg agcgccgcgc ttgaccgcgt gatgagcgat gagatcgttc tcgacgaggg
     8941 cgaggcgttc tatcttcacc caggagagct ggcgctggcg gtgacgctgg agtcggtgac
     9001 gctgccagcc gatctggtgg gctggctgga cgggcgttcc tctttggcgc gtctggggct
     9061 gatggtgcac gtcaccgcgc accgtatcga tccgggctgg tctggttgca ttgtgctgga
     9121 gttctacaac tccggtaagc tgccgctggc gctgcgtccg ggcatgttaa ttggtgcgct
     9181 gagctttgag ccgctttccg gcccggcggc gcgaccttac aaccgccgtg aagatgcgaa
     9241 atatcgcaac cagcagggcg cggtagccag ccgaatcgat aaagactaat tcaggcccat
     9301 tgaggatacc atgagacgat ttctgacgac gctgatgata ctcctggtcg tgctggtggc
     9361 cgggttatct gcgttagtgt tgctggtgaa tccgaatgat ttccgcgact atatggtcaa
     9421 gcaagttgct gcacgtagcg gttatcaatt gcagctcgac gggccactgc gttggcacgt
     9481 ctggccgcag cttagtatcc tctccgggcg aatgtctctc accgcccagg gcgcaagcca
     9541 gccactggtt cgcgccgaca acatgcgtct ggacgtggcg cttttaccac tactgagtca
     9601 tcaactgagc gttaagcagg tgatgctaaa aggggcagtg atccaactga cgccgcagac
     9661 ggaagcggtg cgcagtgaag acgctccggt tgcaccgcgc gacaatacct tgccggatct
     9721 gtcagacgat cgcggatggt cgtttgatat atccagtctt aaggtggcgg acagcgtgct
     9781 ggtgttccag catgaagatg acgagcaggt gacaatccgc aatatccgcc tgcaaatgga
     9841 acaagatccc caacatcgtg gctcatttga gttctccggg cgggttaatc gcgatcagcg
     9901 cgatctcacg atatccctta acggtacggt agatgcttct gattatccgc atgatttaac
     9961 ggcggctatt gaacaaatta actggcagtt gcagggtgca gatttaccaa aacaaggtat
    10021 tcaggggcag gggagtttcc aggcccagtg gcaggagtca cataaacgcc tttcatttaa
    10081 ccaaatcagc ttgaccgcca atgacagcac gctgagcggg caagcacagg tcacgctgac
    10141 agagaaacct gaatggcagc tgagactgca atttccgcaa ctgaatcttg acaacctcat
    10201 cccgcttaat gaaacagcga atggtgaaaa cggtgccgcg cagcaggggc agagccaatc
    10261 aacattgccg cgcccggtca tttcttcgcg tattgatgaa ccggcatatc agggactgca
    10321 aggctttacg gctgatattt tgttgcaggc cagtaacgtg cgctggcgcg gaatgaattt
    10381 tacagatgtt gccacgcaaa tgaccaacaa gtcgggtttg ctggaaatta ctcaactgca
    10441 gggcaagctt aacggtggac aggtttcact gccgggcacg ctggacgcga catcaataaa
    10501 tccgcggata aacttccagc cacggctgga aaacgttgag attggtacca ttctgaaggc
    10561 gtttaactat ccgatttcgt tgaccggaaa aatgtcactg gctggtgatt tctccggtgc
    10621 tgacatagat gccgacgcat tccgccacaa ctggcaagga caggcacatg tcgaaatgac
    10681 cgacacgcgc atggaaggga tgaacttcca gcagatgatt cagcaagcgg tagagcgtaa
    10741 tggcggtgat gtgaaggccg ctgaaaactt tgataacgtg acgcggcttg accgctttac
    10801 caccgatttg acgttgaagg atggcgtcgt gacgttaaac gacatgcaag gtcaatcgcc
    10861 tgtgctggcg ctgacagggg aaggcatgtt gaatctggca gatcaaacct gcgacaccca
    10921 gtttgatatt cgggtcgtgg gtggctggaa cggggaaagc aaactgattg atttcctcaa
    10981 agaaacgcca gtaccgctgc gggtttatgg caactggcag caactcaatt acagtctgca
    11041 agtggatcag ttactgcgca aacatctaca ggacgaagcg aaacgtcgcc tgaatgactg
    11101 ggccgagcgg aataaagatt cccgcaatgg caaagatgtg aagaagttgc tggagaagat
    11161 gtaggttacc aggtgacctg tcctgtaggc ctgataagac gcgtaaagcg tcgcatcagg
    11221 cgttcgcggc ggcaccatta tattctgaat atgtaagatc gaaggctgtt ccgttcactt
    11281 gatgttcctc actactatgg aacgctcaag ccggtgaacg tgccagggtg gctcaatcgc
    11341 caccaccctg acaacccggg ctcccggcgg taaatcgccg ctggcgcggt tccctcatct
    11401 tattcctttc ggctatcggg taaaatgacc gataccatca cacctcgtac tccaactcgc
    11461 catccttatg cagcggaata atctgcacct tctgcacgcg atggctttcc acctgcaacg
    11521 ttttaagcaa ataatccccc acctgaactt cttcgccagg ttttggaata cgctgtaaat
    11581 attccatcaa cagcccggca atggtgtgat attcacgttt ttcatccagc ggcagcggca
    11641 catattgcac cagatcttcc agcggcatat gaccattcgc cgtccaggaa ccgtccgcat
    11701 tcttctgaat atcatggcgg gcgtcgatct cttccacttc gttcggtaag ttaccggcaa
    11761 tggtttcagt gacgtcactt aatgtcacaa tcccttccac cgagccaaac tcatccacca
    11821 caaaagcaaa gtgcgtgcgg gcattacgga actgctccag ggcaggtaac aacggcaagg
    11881 tttccgggaa caccagcggc tggcgaatca acacccgcag gttgagcggt tcgccgcgca
    11941 gtgactgttg tagcaggtcg ataacgtgaa caacaccgag caaatcttct gcatcatcac
    12001 cgtcggtaac caccagccgc gtatgctgat ttcgctccag taactggcgg atctcgtctt
    12061 ccggcgcgtt gagatcgata tgctcaatat cgtggcgcga cgtcataatg ctgctgacgg
    12121 tacgctggtt aagattaagt acccgctcaa tcatccgccg ttcctgcgga ttaaagatct
    12181 gctggttacc atgatccacc agcatggacg cggtttcggc gtccagttcc gcatcttctt
    12241 tttgcccgct aagcaggcgc attaccgctt cggtagtccg ctggcgcagc gtctggttag
    12301 cggaaagaaa acgtcgccgg ttaaagatag ccaactgatt gagcgcctcg atcatcaccg
    12361 agaaaccaat ggcagcgtac aggtagcctt tcggaatgac gaagccgaaa ccttctgcca
    12421 ccaggctaaa gccaatcatc aacaggaagc ttaagcagag aataacgatc gtcgggtgac
    12481 tgttaacaaa ttgcgttaac ggcttgctgg ccatcaacat caggctgata gcgataacca
    12541 cggcggccat catgaccagt aaatggtcga ccatcccgac ggcggtaatc actgagtcga
    12601 gtgagaaaat ggcgtccagt accacaattt gcgtcaccac gccccagaac ttcgcacctt
    12661 tgcgttgtgt gggattattg ctgtctttcc cttccagccg ttcgttcagc tccatcgtgg
    12721 ctttgaacaa caggaagaaa ccaccaaaca gcatgattaa gtcgcgggcg ctaaaggtaa
    12781 acgagcggaa gctgaacagc ggttgagtca gggtgaccag ccaggagatt gacgccagca
    12841 gtaacaggcg cattaacatt gccagcagca gcccggtaac acgtgcgcgg tcacgctgct
    12901 tcggcggtag tttttcggcg aggatggcaa taaagaccag gttatcaatg ccgaggacca
    12961 gttcgatcac aatcagcgtg attaacccgg cccagataga cggatcggca atccattcca
    13021 tagtaagtac aaaacctatt cgttgtatga caaatgtcac aattcgatca tggataaatc
    13081 ggggcaaaaa tgcaatgttg cagcactatt tatctcatat tatttgaggg taataacctc
    13141 acattatccc tgaattaaaa gtggtaat
//
LOCUS       DAFNBJ010000132        13164 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000132 DAFNBJ010000000
VERSION     DAFNBJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.132"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            157..492
                     /locus_tag="L2X49_003658"
     CDS             157..492
                     /locus_tag="L2X49_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBN4798679.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCRIDGFGPMK
                     LKSEFVKKN"
     gene            652..1095
                     /gene="yjdN"
                     /locus_tag="L2X49_003659"
     CDS             652..1095
                     /gene="yjdN"
                     /locus_tag="L2X49_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBN4798680.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1228..2016
                     /gene="phnC"
                     /locus_tag="L2X49_003660"
     CDS             1228..2016
                     /gene="phnC"
                     /locus_tag="L2X49_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBN4798681.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2041..3057
                     /gene="phnD"
                     /locus_tag="L2X49_003661"
     CDS             2041..3057
                     /gene="phnD"
                     /locus_tag="L2X49_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN4798682.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKMIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3119..3898
                     /gene="phnE"
                     /locus_tag="L2X49_003662"
     CDS             3119..3898
                     /gene="phnE"
                     /locus_tag="L2X49_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBN4798683.1"
                     /translation="MQTITIAPSKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVIPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3919..4644
                     /gene="phnF"
                     /locus_tag="L2X49_003663"
     CDS             3919..4644
                     /gene="phnF"
                     /locus_tag="L2X49_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBN4798684.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNTSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4645..5097
                     /gene="phnG"
                     /locus_tag="L2X49_003664"
     CDS             4645..5097
                     /gene="phnG"
                     /locus_tag="L2X49_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBN4798685.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5094..5678
                     /gene="phnH"
                     /locus_tag="L2X49_003665"
     CDS             5094..5678
                     /gene="phnH"
                     /locus_tag="L2X49_003665"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBN4798686.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5678..6742
                     /gene="phnI"
                     /locus_tag="L2X49_003666"
     CDS             5678..6742
                     /gene="phnI"
                     /locus_tag="L2X49_003666"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBN4798687.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6735..7580
                     /gene="phnJ"
                     /locus_tag="L2X49_003667"
     CDS             6735..7580
                     /gene="phnJ"
                     /locus_tag="L2X49_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBN4798688.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7577..8335
                     /gene="phnK"
                     /locus_tag="L2X49_003668"
     CDS             7577..8335
                     /gene="phnK"
                     /locus_tag="L2X49_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBN4798689.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8512..9192
                     /gene="phnL"
                     /locus_tag="L2X49_003669"
     CDS             8512..9192
                     /gene="phnL"
                     /locus_tag="L2X49_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBN4798690.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9189..10325
                     /gene="phnM"
                     /locus_tag="L2X49_003670"
     CDS             9189..10325
                     /gene="phnM"
                     /locus_tag="L2X49_003670"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBN4798691.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQNRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10325..10882
                     /gene="phnN"
                     /locus_tag="L2X49_003671"
     CDS             10325..10882
                     /gene="phnN"
                     /locus_tag="L2X49_003671"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBN4798692.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10869..11303
                     /gene="phnO"
                     /locus_tag="L2X49_003672"
     CDS             10869..11303
                     /gene="phnO"
                     /locus_tag="L2X49_003672"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBN4798693.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11305..12063
                     /gene="phnP"
                     /locus_tag="L2X49_003673"
     CDS             11305..12063
                     /gene="phnP"
                     /locus_tag="L2X49_003673"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HBN4798694.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVVPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12210..12539)
                     /gene="yjdP"
                     /locus_tag="L2X49_003674"
     CDS             complement(12210..12539)
                     /gene="yjdP"
                     /locus_tag="L2X49_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HBN4798695.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12608..13057)
                     /gene="rpiB"
                     /locus_tag="L2X49_003675"
     CDS             complement(12608..13057)
                     /gene="rpiB"
                     /locus_tag="L2X49_003675"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HBN4798696.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         2901 a         3982 c         3666 g         2615 t
ORIGIN      
        1 attttctttt tgcgtttcct ttcaaatgaa aacgatcgtc gtctaaaatc agcagtaccc
       61 ccgacaaact cagggatttt gtgtataatt gcggcctttt tcggcaatct gccgtttttt
      121 ggcgcttttg ccctgctgac ttttgaggaa atccacatgt cattaccaca ctgcccaaaa
      181 tgcaactccg aatacactta cgaagataac ggcatgtaca tctgcccgga atgtgcctac
      241 gaatggaacg acgcagaacc tgcacaggaa agcgacgagc tgatcgttaa agatgctaac
      301 ggcaatctgc tggctgacgg cgacagcgtt accatcatta aagatctgaa ggtgaaaggt
      361 agctcttcga tgctgaaaat tggcaccaaa gtgaaaaaca tccgcctggt tgaaggcgac
      421 cataacatcg attgcagaat cgacggtttt ggtccgatga aactgaaatc tgagtttgtg
      481 aaaaagaact gattgtattg tgatcggtaa gccggataag gcgctcgcgc cgcatccggc
      541 aacgatgccg actgcctgat gcgacgctga ccgcgtctta tcaggcttcc ctctcatctg
      601 tataaatttc gaactacact taactggctt ctcttaactg aggtcaccat catgccgtta
      661 agtccctacc tctcttttgc cggtaactgt gccgacgcaa ttgcttatta tcaacgtacg
      721 ttgggcgctg aactgctcta taaaatcagc ttcggcgaaa tgccaaaatc agcgcaggac
      781 agcgccgaga actgcccttc cggaatgcaa tttcccgata ccgccatcgc tcatgccaac
      841 gtgcgcattg ccggaagcga catcatgatg agcgatgcca tgccgtcagg aaaagccagc
      901 tactccggct ttacgctggt gctcgattcg caacaggtcg aagaaggaaa acgctggttt
      961 gacaatcttg ccgctaacgg aaaaatcgaa atggcctggc aggaaacttt ctgggcgcat
     1021 ggctttggca aagtcaccga taaatttggc gtaccgtgga tgattaatgt cgtcaaacaa
     1081 caaccaacgc aataatccgc cgggaggccc gccctcccgc actgtcatcg aattcccgtt
     1141 aactcttcat ctgttagtca cttttaatta accaaatcgt cacaataatc cgccacgatg
     1201 gagccacttt tttagggagg ctgcatcatg caaacgatta tccgtgtcga gaagctcgcc
     1261 aaaaccttca atcagcatca ggcgctgcat gcggttgatc tgaacattca tcacggtgaa
     1321 atggtggctc tgcttgggcc gtcgggttcc ggcaaatcca cccttttacg tcacttaagc
     1381 ggtttgatta ccggcgataa atccgccggc agccatatcg agctgctggg ccgcacagtc
     1441 cagcgcgaag gccgtctggc gcgcgatatc cgcaaaagcc gcgccaacac cggctacatc
     1501 ttccaacaat tcaacctggt gaaccgcctg agcgtactgg agaacgtgct gattggcgcg
     1561 ctcggcagca cgccgttctg gcgcacctgt tttagctggt ttacccgcga gcagaaacaa
     1621 cgcgcgttac aggcgctgac ccgcgttggc atggtgcatt ttgcccatca acgcgtttcc
     1681 accctctccg gcggacagca gcagcgtgtg gcgattgccc gcgcgctgat gcagcaggcg
     1741 aaggtgattc tggccgatga acccatcgcc tcgctggacc cggaatccgc ccgcatcgtg
     1801 atggacaccc tgcgcgacat caatcagaac gacggcatca ccgtggtcgt cacgctgcat
     1861 caggtggatt acgccctgcg ctactgcgaa cgcatcgtcg ccctgcgcca ggggcacgtt
     1921 ttctacgacg gcagcagcca acagtttgat aacgaacgtt ttgaccatct ctaccgcagc
     1981 attaatcgca tcgaagagaa cgcgaaagct gcctgacatc cccatcattg aggaaaacga
     2041 atgaacgcta agataattgc ctcgctggcc ttcaccagca tgttcagcct cagcaccctg
     2101 ttaagcccgg cacacgccga agagcaggaa aaggcgctga atttcggcat tatttcaacg
     2161 gaatcacagc aaaacctgaa accgcaatgg acgccattct tacaggatat ggagaagaag
     2221 ctgggcgtga aggtgaacgc cttctttgcc ccagactacg caggcattat ccagggaatg
     2281 cgcttcaata aagtggatat cgcctggtac ggcaacctgt cggcaatgga agcggtggat
     2341 cgcgccaacg gccaggtctt cgcccagacg gtcgcggcgg atggatcgcc aggttactgg
     2401 agcgtgttga tcgtcaacaa agatagtccg atcaacaacc tgaacgatct gctggcgaag
     2461 cggaaagatc tcaccttcgg caatggcgat cctaactcca cctctggctt cctcgtcccc
     2521 ggttactacg tctttgccaa aaacaatatc tccgccagcg actttaagcg caccgtcaac
     2581 gctgggcatg aaaccaacgc gctggccgtc gccaacaagc aggtggatgt tgccaccaac
     2641 aacaccgaaa acctcgacaa gctgaaaacc tccgcgccag agaagctgaa agaactgaag
     2701 atgatctgga agtcgccgct gatcccaggc gatccgatcg tctggcgcaa gaatctctcc
     2761 gaaaccacca aagacaagat ctacgacttc tttatgaact acggaaaaac gccggaagag
     2821 aaagcggtgc tggaacgcct gggctgggcc ccgttccgcg cctccagcga cctgcaactg
     2881 gtgccgattc gccagctcgc actgtttaaa gagatgcagg gcgtaaaaag caataaagga
     2941 ctgaatgagc aggacaagct ggcgaaaacc accgagattc aggcgcagct ggatgacctg
     3001 gaccgcctga acaacgcgtt aagcgcgatg agttcggtga gtaaagcggt gcagtaatgt
     3061 gctcgatcgg tcccctctcc cttgagggag agggagaaaa cattaaacgg agccaaacat
     3121 gcaaaccatc accatcgccc catccaagcg tagctggttc tcgcttctga gctgggccgt
     3181 tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa atggccccgc ttacgctgat
     3241 taaagacggc ggcaacatgg caaccttcgc tgccgacttc ttcccgcccg atttcagcca
     3301 gtggcaggat tacctcaccg aaatggccgt cacgctgcaa atcgccgtct ggggcaccgc
     3361 gctggcagtg gttctctcca tccccttcgg cctgatgagc gccgaaaacc tggtgccgtg
     3421 gtgggtttac cagcccgttc gccgcctgat ggacgcctgc cgcgccatta acgaaatggt
     3481 cttcgccatg ctgttcgtgg tcgccgtcgg tctcggaccg ttcgctggcg tgctggcgct
     3541 atttattcac accaccggcg tgctctcgaa actgctttcc gaagcggtgg aagcgattga
     3601 acccggcccg gtggaaggca ttcgcgccac cggcgccaac aagctggaag agatccttta
     3661 cggcgtgctg ccacaggtaa taccgctgct gatctcctac tccctctatc gcttcgaatc
     3721 caacgtccgc tcggcgaccg tcgtcggcat ggtcggtgca ggcgggatcg gcgtcaccct
     3781 gtgggaagcg attcgcggtt tccagttcca gcaaacctgc gccctgatgg tgcttatcat
     3841 cgtcacggtc agcctgctgg atttcctctc tcaacggttg cgtaagcact ttatctgata
     3901 agcgaggcat tgatatctat gcacttgtct acacatccga ccagctaccc aacacgctat
     3961 caggagatag ccgcaaaact tgagcaggag cttcgtcaac actaccgctg cggcgactat
     4021 cttcccgccg agcagcaact ggcggcgcgc tttgaggtga accgccacac cctgcgccgc
     4081 gccatcgacc aactggtgga aaaaggctgg gtacagcgcc gtcagggcgt cggcgtgctg
     4141 gtgctgatgc gcccgttcga ttacccgctc aacgcccagg cgcgttttag ccagaacctg
     4201 ctggaccagg gtagccatcc caccagcgaa aaactgctgt cggtattgcg ccccgcgtcc
     4261 ggtcacgtcg ctgacgcgct ggggattacc gagggcgaga acgttatcca tttgcgcacc
     4321 ctgcgtcgcg tcaacggcgt cgcgctctgt ttaatcgacc actactttgc ggacctcacc
     4381 ctttggccga cgctgcaacg cttcgacagc ggttcgctgc acgattttct gcgcgagcaa
     4441 accggaattg cgctgcgccg cagccagacg cggatcagcg cccgtcgcgc ccaggccaaa
     4501 gagtgccagc gtcttgaaat tccgaatacg tcgccgctgc tgtgcgtgcg cacccttaac
     4561 caccgtgacg gtgaaagcag cccggcggag tactccgtca gcctgacgcg cgccgacatg
     4621 attgaattca ctatggagca ctgaatgcac gcagataccg cgacccgcca gcactggatg
     4681 tccgtactgg cgcacagcca accggctgaa ctggcagccc gcctgaaagc actgaacatc
     4741 accgccgact atgaggttat ccgcgccgcc gaaaccggcc tggtgcagat tcaggcgcgg
     4801 atgggcggca ccggcgaacg cttttttgcc ggcgacgcca cgctcacccg cgccgccgta
     4861 cgcctgactg acggcacgct cggctacagc tgggtgctgg ggcgcgacaa acagcacgcc
     4921 gaacgctgcg cgctgattga cgcgctgatg cagcaatctc gctattttca aaacttatca
     4981 gaaaccctta ttgccccgct ggacgctgac cgtatggcac gcattgccgc acgccaggcc
     5041 gaagtgaacg ccagccgggt cgacttcttt acgatggttc gcggagacaa cgcatgaccc
     5101 tggaaaccgc ttttatgctt cccgtgcagg atgcccagca cagttttcgt cgcctgttaa
     5161 aggccatgag cgagccgggc gtgattgtcg ccctgcatca gctcaagcgc ggctggcagc
     5221 cgctgaatat cgccaccacc agcgtgctgc tgacgctggc cgataacgac acgccggtgt
     5281 ggcttgccgc gccattgagt aacgatatcg tcagccagag cctgcgtttt cataccaacg
     5341 cgccgctggt cagccagccg gaacaggcga ccttcgcggt gacggatgag gcgatttcca
     5401 gcgaacagct taacgccctt tccaccggca ccgccgttgc gccggaagcg ggtgcaacgc
     5461 tgattttaca ggtcgccagc ctgagcggcg ggcgcatgct gcgccttact ggcgcgggta
     5521 ttgccgaaga acgaatgatc gctccgcagc tgccggagtg cattctgcac gaactcaccg
     5581 agcgcccgca cccgttcccg ctcggcatcg acctgatcct gacctgcggc gagcggctgc
     5641 tggctatccc gcgaaccact catgtggagg tgtgctgatg tacgttgccg tgaaaggggg
     5701 cgagaaggcg atcgacgccg cccacgccct gcaagagagc cgacgccggg gcaataccga
     5761 tttgcctgaa ctgagcgtcg cccagattga acagcagctt aatcttgcag tagatcgcgt
     5821 gatgaccgaa ggcggcattg ccgaccgcga actggcggcg ctggcgctga aacaggccag
     5881 cggcgataac gtcgaagcga ttttcctgct acgcgcctac cgcaccacgc tggcgaagct
     5941 ggcggtaagc gagccgctcg acaccacgga gatgcgtctc gaacggcgta tttcagcggt
     6001 ttataaagat attcccggcg gtcagttgct tggcccaacc tacgactaca cccatcgcct
     6061 gctcgatttc accctgctgg caaacggcga agcgccgacg ctgaccaccg ccgacagcga
     6121 acaacagccg tcgccgcacg ttttcagcct gctggcgcgt caggggctgg cgaagtttga
     6181 agaagatagc ggcgcacagc cggatgacat cacccgcacg ccgccggttt acccctgctc
     6241 acgctcctcc cgcctgcaac aattaatgcg cggggacgaa ggctatttgc tggcgctggc
     6301 ctactccacc cagcgtggtt acgggcgcaa tcacccgttc gcgggcgaga tccgcagtgg
     6361 ttacatcgac gtgtcgattg tgccggaaga gctgggattt gcggtaaacg tcggcgaact
     6421 actgatgacc gagtgtgaaa tggtcaacgg ttttatcgac ccgccggatg agccgccgca
     6481 cttcacgcgc ggctacgggc tggtattcgg catgagcgag cgcaaagcga tggcaatggc
     6541 gctggtcgat cgtgcgttgc aggctccgga atacggcgag cacgcgacag gcccggcgca
     6601 ggatgaagag tttgtgctgg cacatgccga caacgtcgaa gccgcaggct ttgtctcgca
     6661 cctcaaactc ccccactacg tcgatttcca ggccgaactg gagctactca aacgtctgca
     6721 acaggagaag aaccatggct aatctgagcg gctacaactt tgcctacctc gacgagcaga
     6781 ccaaacgcat gatccgccgc gctatcttaa aagcggtggc gatccccggt tatcaggtgc
     6841 cgtttggcgg gcgcgagatg ccgatgccat acggctgggg aaccggcggc atacagctca
     6901 ccgccagcgt gattggcgaa agcgacgtgc tgaaggtgat tgaccagggt gcggatgaca
     6961 ccaccaacgc cgtgtcgatt cgcaacttct ttaagcgcgt gaccggggta aacaccactg
     7021 aacgtacgga cgatgcgacg cttatccaga cgcgtcaccg catccccgaa acgccgctga
     7081 ccgaagatca gatcattatc ttccaggtgc caatcccgga accgctgcgc tttatcgagc
     7141 cgcgcgaaac ggaaacccgc accatgcacg cgctggaaga gtacggcgtg atgcaggtga
     7201 aactgtatga agatatcgcc cgcttcggtc atatcgccac tacctacgcc tatccggtga
     7261 aggtgaacgg gcgctacgta atggacccgt cgccgatccc gaaattcgat aacccaaaaa
     7321 tggacatgat gcccgccctg caactgttcg gcgcggggcg cgagaagcgc atctatgcgg
     7381 tgccgccgtt tacccgcgtg gaaagtctcg atttcgacga tcacccgttc accgttcagc
     7441 agtgggatga gccatgcgcc atctgcggat cgacccacag ctatcttgat gaagtggtgc
     7501 tggatgacgc cggaaaccgc atgtttgtct gctccgatac cgattattgc cgccaacaga
     7561 gcgaggcaaa aaaccaatga atcaaccgtt actttcggtc aataacctga cccaccttta
     7621 cgcgccgggc aaaggcttta gcgatgtctc ttttgattta tggccggggg aagtgctggg
     7681 cattgtcggg gagtccggct ccgggaagac cacgctgctg aaatcaatct ccgcgcgcct
     7741 gacgccgcag cagggggaga ttcactatga gagccgttcg ctgtacgcaa tgagcgaggc
     7801 cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg catcagcatc cgctcgacgg
     7861 cctgcgccgc caggtctcgg caggcggcaa tatcggcgag cggctgatgg cgaccggggc
     7921 gcgtcattac ggcgacattc gcgccaccgc gcagaagtgg ctggaagagg tggaaattcc
     7981 cgccaaccgg atcgacgacc tgccgaccac cttttccggc ggcatgcagc aacgtttgca
     8041 gatcgcccgc aacctggtga cgcatccgaa gctggtgttt atggacgagc cgaccggcgg
     8101 actggatgtg tcggtacagg cacgcctgct cgacctgctg cgcggcctgg tggtggagct
     8161 gaacctcgcg gtagtgattg tcacccacga tttaggcgtc gcccgcctgc tggcggaccg
     8221 tttgctggtg atgaagcagg ggcaagtggt ggagagtggg ttaaccgacc gcgtgctcga
     8281 cgacccgcac catccgtata cacaactgct ggtgtcatcg gttttgcaga attgaatgca
     8341 acaccgtgcg cgctgatccc ctcaccccgg ccctctcccg gagggagagg gagaaaaccg
     8401 gcgcaatatt caacaccgcg cacaatcggt cccctcgccc cttcggggag agggttaggg
     8461 tgaggggcaa tgcgcgcacc gctccccaat atgcgaacca cgaggcgaaa aatgattaac
     8521 gtacaaaacg tcagtaaaac cttcatcctg caccagcaaa acggcgtgcg cctgcccgtc
     8581 ttacaacgcg cctcgctcac cgtcaacgca ggtgaatgcg tggtgctcca cggccattcc
     8641 ggcagcggca aatcaactct gctacgctcg ctgtacgcca actatctgcc cgacgaaggg
     8701 caaatccaga tcaaacacgg tgacgaatgg gtagacctgg tcaccgcgcc agcgcgcaaa
     8761 gtggtggaga tccgcaaaac taccatcggc tgggtgagcc agtttctgcg cgtcatcccg
     8821 cgtatctcag cactggaagt ggtgatgcag ccgctgctcg atacaggcgt tccgcgtgaa
     8881 gcctgcgccg ctaaagccgc gcgtcttctc acccgcctga acgtgccgga acgcctgtgg
     8941 cacctggcac catcgacctt ctccggcggc gaacagcagc gcgtcaacat cgcccgcggc
     9001 tttatcgtcg actaccccat tctgctgctc gacgaaccta ccgcctcgct ggacgccaaa
     9061 aacagcgccg cggtggtgga actgattcac gaagccaaag cccgtggcgc agccatcgtc
     9121 ggcatcttcc atgacgaagc tgtacgtaat gacgtcgccg accgcctgca cccaatggga
     9181 gcctcttcat gattatcaat aacgttaagc tggtgctgga aaacgaggtg gtgcacggtt
     9241 cgctggaggt gcaggatggc gaaatccgcg cctttgccga aagccagaac cgcctgccag
     9301 aagcgatgga cggcgaaggt ggctggctac tacccggcct gattgagctg cataccgaca
     9361 acctcgataa attcttcacc ccgcgcccga aagtcgactg gcccgcccat tcggcgatga
     9421 gcagccacga cgcgctgatg gtggcaagcg gcatcaccac cgtactggac gccgtggcga
     9481 ttggtgatgt gcgcgacggc ggcgatcggc tggagaatct ggagaagatg atcaacgcca
     9541 tcgaagagac gcaaaaacgc ggcgtcaacc gcgccgagca ccgcctgcac ctgcgctgcg
     9601 aactgccgca tcacaccaca ctgccgctgt ttgaaaaact ggttcagcgc gaaccggtga
     9661 cgctggtgtc gctgatggac cactcaccgg gccagcgcca gttcgctaac cgcgagaagt
     9721 atcgcgaata ttatcagggc aaatactccc tcaccgatgc gcagatgcag cagtacgaag
     9781 aagagcaatt ggcgctcgcc gcacgctggt cgcagccgaa tcgcgaatcc atcgccgccc
     9841 tgtgccacgc gcgacaaatt gcgctcgcca gccacgatga cgccacgcac gcccacgttg
     9901 ccgaatccca ccaacttggc agcgtgatcg ccgaatttcc caccacgttc gaagcggcgg
     9961 aagcctcgcg taagcatggc atgaacgtgc tgatgggtgc gccaaatatc gtgcgtggcg
    10021 gctcgcactc cggcaatgtg gcggccagtg aactggcgca gcttggtctg ctggatatcc
    10081 tctcttccga ctactacccc gccagcctgc tggatgcggc gttccgcgtc gccgatgacg
    10141 agagcaaccg ctttacgcta ccgcaggcgg tgaggctggt gaccaaaaat ccggcgcagg
    10201 cgctgaatct tcaggatcgc ggggtgattg gcgagggtaa acgcgctgac ctggtgctgg
    10261 cgcatcgcca gggcaatcac attcatatcg accacgtctg gcgtcagggt aaaagggtgt
    10321 tctgatgatg ggaaaactga tttggttaat ggggccgtcc ggctccggga aagacagcct
    10381 gctggcggaa ctccgcctgc gggaacaaac gcagttactg gtggcgcatc gctacatcac
    10441 gcgcgacgcc agcgccggaa gtgaaaacca tatcgccctg agtgagcggg agttttttac
    10501 ccgcgcgggg caaaacctgc tggccttaag ctggcacgct aacggtctgt attatggcgt
    10561 cggcatcgag attgacctct ggctgcacgc tggattcgac gtagtcgtca acggttcacg
    10621 cgcgcatttg ccgcaggcac gggcgcgcta tcaatcggcg ctgctgcccg tctgtttaca
    10681 ggtttcgccg gagatcctgc gccagcgcct ggaaaaccgt ggccgtgaaa acgccagtga
    10741 aattaacgcc cgcctggcgc gcgccgcccg ctatactcca caggattgcc atacgctcaa
    10801 caatgacggc agcctgcgcc agtcggtcga cacgctgctg acgctgatcc atcagaagga
    10861 gaaacaccat gcctgcttgt gagcttcgcc cggccacgca gtacgatacc gacgcggttt
    10921 acgcgctgat ttgtgagcta aaacaggcgg agtttgacca ccatgcgttt cgcgtgggct
    10981 ttaacgccaa cctgcgcgac ccaaacatgc gctaccatct ggcgctgctg gatggcgaag
    11041 ttgtcggcat gatcggcctg catttgcagt ttcatctgca tcatgtcaac tggatcggcg
    11101 aaattcagga actggtggtg atgccgcagg cgcgcggtct gaacgtcggc agtaaattac
    11161 tggcatgggc agaagaagaa gcccgccagg ccggggccga aatgaccgag ctttccacca
    11221 acgtgaagcg ccacgacgca caccgttttt acctgcgtga aggctacgag cagagccact
    11281 tccgcttcac caaggcgctg taacatgagc ctgaccctca cgctcaccgg cactggcggc
    11341 gcacagggcg ttccggcatg gggctgcgag tgcgcggcct gcgccagagc gcggcgctcg
    11401 ccgcagtatc gccgccagcc atgcagcggc gtagtgaagt ttaacgacgc gatcaccctg
    11461 atcgacgccg ggcggcacga tctcaccgat cgctggtcgc ctggatcgtt ccagcagttt
    11521 ttgctgacgc actaccatat ggatcacgtc caggggctat ttccgctacg ctggggcgtg
    11581 ggcgatgtga tcccggtcta cggcccgccg gatgaacagg gctgcgacga tctgtttaaa
    11641 catccgggcc tgcttgattt cagccacaca gtggtaccgt ttgtggtgtt tgatttgcag
    11701 gggttgcagg tcacgccgct gccgcttaac cactcaaaac tgaccttcgg ttatctgctg
    11761 gaaacggcac atagccgggt ggcgtggctg tctgacaccg ctggcctgcc ggaaaaaacg
    11821 ctgaaatttt tactcaataa tcatccgcag gtgatggtga ttgattgcag tcacccgccg
    11881 cgcgcggatg caccgcgtaa tcactgtgat ttaaataccg tgcttgcgct gaatcaggtt
    11941 atccgctcgc cacaggtgat tctgacccat atcagccacc agtttgatgc gtggctgatg
    12001 gaaaacgcac taccgtcagg gtttgaggtg gggtttgatg ggatggagat tggggtggcg
    12061 tgatgagagg gaatgtgcgc gctggccccc tcaccctaac cctctcccca gaggggcgag
    12121 gggaccgatt gtgctcgata ttgaatattg cgctcgtttt ctccctctcc ccattggggt
    12181 gaggggcgat gcctgctcca tacccaacct catcgcccat actcatcttc cattctccgc
    12241 tcttcatcct ccagttgccg acgctcctga tcaagctggc gctggcgatc gtccagctgc
    12301 ctgcggcgat cttcaaactg gcggcggcgg tcgtcatatt gtctgcgccg atcgtcgctc
    12361 acttcacgct gccagccgtc gtcgcgcgaa tcttcatagt ctcgcccacg gtcagggtta
    12421 taagcgtcat taatcgcctg ctgaatatta ccaatagtat cgtcgataat atcagcctgg
    12481 gccggaacgg tggacagcgt gagcagggtg aataaaagaa atagcggaaa gcgtttcatt
    12541 agccaacctc aaaaagaaac tctatccaca ttaatcatta ctcatccatg caagtagtgg
    12601 atgaatctca atttctccgc tgctctattg ccgtaatcgc ctccacgcgt tgttgatgac
    12661 gaccgccttc gtactgtgcg cccagccacg catccacaat catttttgcc agttcgaggc
    12721 caaccactcg tgaaccaaaa gccagcacgt tggtgtcgtt atgctgccgc gaaagttgcg
    12781 cggaataagg ttcgctacag acgaccgcgc gaattccggc aaacttgttc gccgctatcg
    12841 aaataccgac gccagtacca caaatcaaaa tcccgccatc aacctctccg ccagcaacag
    12901 ccagtgcgac ttgactggcg taatgcggat aatcagtacg ctctgacgac caggttcctt
    12961 tatcaatcac ttcaacgcca cgctcaacta aatgtgccac tatttcatgt tttaaaatga
    13021 aaccgacatg atcacagcca aatgcaatct ttttcattca tcttccccgt tcgtgcaaaa
    13081 acttcacaat caaaaaacat ttgtgatgta aatgaattaa aaacacccaa aatcgatgaa
    13141 atccgccttc ctgatctgtt ttac
//
LOCUS       DAFNBJ010000133        13009 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000133 DAFNBJ010000000
VERSION     DAFNBJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.133"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            271..837
                     /gene="yqaB"
                     /locus_tag="L2X49_003676"
     CDS             271..837
                     /gene="yqaB"
                     /locus_tag="L2X49_003676"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBN4798697.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            834..1262
                     /locus_tag="L2X49_003677"
     CDS             834..1262
                     /locus_tag="L2X49_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBN4798698.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1335..2891
                     /gene="gshA"
                     /locus_tag="L2X49_003678"
     CDS             1335..2891
                     /gene="gshA"
                     /locus_tag="L2X49_003678"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBN4798699.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            3041..3556
                     /gene="luxS"
                     /locus_tag="L2X49_003679"
     CDS             3041..3556
                     /gene="luxS"
                     /locus_tag="L2X49_003679"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBN4798700.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="L2X49_003680"
     CDS             complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="L2X49_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBN4798701.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="L2X49_003681"
     CDS             complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="L2X49_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HBN4798702.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGLEEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="L2X49_003682"
     CDS             complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="L2X49_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBN4798703.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="L2X49_003683"
     CDS             complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="L2X49_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBN4798704.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALTYG
                     LVWKVMAII"
     gene            complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="L2X49_003684"
     CDS             complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="L2X49_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBN4798705.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8281..9465)
                     /locus_tag="L2X49_003685"
     CDS             complement(8281..9465)
                     /locus_tag="L2X49_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4798706.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9757..10749)
                     /gene="proX"
                     /locus_tag="L2X49_003686"
     CDS             complement(9757..10749)
                     /gene="proX"
                     /locus_tag="L2X49_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBN4798707.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10807..11871)
                     /gene="proW"
                     /locus_tag="L2X49_003687"
     CDS             complement(10807..11871)
                     /gene="proW"
                     /locus_tag="L2X49_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBN4798708.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11864..>13009)
                     /gene="proV"
                     /locus_tag="L2X49_003688"
     CDS             complement(11864..>13009)
                     /gene="proV"
                     /locus_tag="L2X49_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBN4798709.1"
                     /translation="RAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLS
                     GSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHM
                     TVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARAL
                     AINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAI
                     MQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFG
                     PRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQ
                     TPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT         3238 a         3359 c         3435 g         2977 t
ORIGIN      
        1 aaccttcagc aatgaaggta tcgaagagat agcggtatta accgcaggca ccagggagga
       61 taacgttgcg tcagcaacgg cccgaagggc gaacgccagt gagtaatcct cccggatgca
      121 ccatcttctc ccaaaaattt ccttcaaaaa tatctcattt ctaaataact actttatcca
      181 tttcctgaat tttcttctcc ttcgccatca gcgacaaaat caagcaatta cgctaaagta
      241 gcgtcatatt tttcgacttg cgagaagacc atgtacgagc gttatgcagg tttaattttt
      301 gatatggatg gcacaatcct ggatacggag cctacgcacc gtaaagcgtg gcgcgaagta
      361 ttagggcact acggtcttca gtacgatgtt caggcaatga ttgcgcttaa tggatcgccc
      421 acctggcgta ttgctcaggc aattattgag ctgaatcagg ccgatctcga cccgcatgcg
      481 ttagcgcgtg aaaaaacaga agcagtaaga agtatgctgc tggatagtgt cgagccgctt
      541 cctcttgttg aagtggtgaa aagttggcat ggtcgtcgcc caatggctgt aggaacgggg
      601 agtgaaagcg ccatcgctga ggcattgctg gcgcacctgg gattacgccg ttattttgac
      661 gccgtcgtcg ctgctgatca cgtcaaacac cataaacccg cgccagacac atttttgttg
      721 tgcgcgcagc gtatgggcgt gcaaccgacg cagtgtgtgg tctttgaaga tgccgatttc
      781 ggtattcagg cggcccgtgc agcaggcatg gacgccgtgg atgttcgctt gctgtgagtg
      841 aagcgttatc gcttttctcg ttgtttgcca gtagttttct cagcgctaca ctattacccg
      901 gcaactccga agtcgtgctg gtggcaatgt tgctttccgg gatcagttat ccctgggttt
      961 tagttttaac agcaacaatg ggtaatagcc ttggagggtt aactaacgtt atccttgggc
     1021 gtttctttcc attgcgtaaa acatcgcgct ggcaagagaa agctaccggc tggctgaaac
     1081 gctatggtgc agtcacgcta ttattaagct ggatgcccgt ggttggcgat ttactgtgtc
     1141 tgttagcggg atggatgcgc atctcctggg ggccagtaat cttttttttg tgccttggta
     1201 aagcgttacg ctatgttgca gttgcagcag cgaccgttca gggcatgatg tggtggcact
     1261 aattgtaggc ctgcacatat ggtcaccatt acagttatgc taattaaaac gattttgaca
     1321 ggcgggaggt caatttgatc ccggacgtat cacaggcgct ggcctggctg gaaaaacatc
     1381 ctcaggcgtt aaaggggata cagcgtgggc tggagcgcga aactttgcgt gttaatgctg
     1441 atggcacact ggcaacaaca ggtcatcctg aagcattagg ttccgcactg acgcacaaat
     1501 ggattactac cgattttgcg gaagcattgc tggaattcat tacaccagtg gatggtgata
     1561 ttgaacatat gctgaccttt atgcgcgatc tgcatcgtta tacggcgcgc aatatgggtg
     1621 atgagcggat gtggccgtta agtatgccat gctacatcgc agaaggtcag gacatcgaac
     1681 tggcacagta cggcacttct aacaccggac gctttaaaac gctgtatcgt gaagggctga
     1741 aaaatcgcta cggcgcgctg atgcaaacca tttccggcgt gcactacaat ttctctttgc
     1801 caatggcatt ctggcaagcg aagtgcggtg atatctcggg cgctgatgcc aaagagaaaa
     1861 tttctgcggg ctatttccgc gttatccgca attactatcg tttcggttgg gtcattcctt
     1921 atctgtttgg tgcatctccg gcgatttgtt cttctttcct gcaaggaaaa ccaacgtcgc
     1981 tgccgtttga gaaaaccgag tgcggtatgt attacctgcc gtatgcgacc tctcttcgtt
     2041 tgagcgatct cggctatacc aataaatcgc aaagcaatct tggtattacc ttcaacgatc
     2101 tttacgagta cgtagcgggc cttaaacagg caatcaaaac gccatcggaa gagtacgcga
     2161 agattggtat tgagaaagac ggtaagaggc tgcaaatcaa tagcaacgtg ctgcagattg
     2221 aaaacgaact gtatgcgccg attcgtccaa aacgcgttac ccgcagcggc gagtcacctt
     2281 ctgatgcgct gttacgtggc ggcattgaat atattgaagt gcgttcgctg gacatcaacc
     2341 cgttctcgcc gattggtgta gatgaacagc aggtgcgatt cctcgacctg tttatggtct
     2401 ggtgtgcgct ggctgatgca ccggaaatga gcagtagcga acttgcctgt acgcgcgtta
     2461 actggaaccg ggtgatcctt gaaggtcgca aaccgggcct gacgctgggt atcggctgcg
     2521 aaaccgcgca gttcccgtta ctgcaggtgg gtaaagatct gttccgcgat ctgaaacgcg
     2581 tcgcgcaaac gctggatagc attaacggcg gcgaagcgta tcagaaagtg tgtgatgaac
     2641 tggttgcctg cttcgataat cccgatctga ctttctctgc ccgtatctta aggtctatga
     2701 ttgatactgg tattggcgga acaggcaaag cgtttgcaga agcctaccgt aatctgctgc
     2761 gtgaagagcc gctggaaatt ctgcgcgaag aggattttgt agccgagcgc gaggcatctg
     2821 aacgccgtca gcaggaaatg gaaaccgctg ataccgaacc gtttgcggtg tggctggaaa
     2881 aacacgcctg acagaaaaga aaaaggccac tcgtgagtgg ccaaaatttc atctctgaat
     2941 tcagggatga tgataacaaa tgcgcgtctt tcatatactc agactcgcct gggaagaaag
     3001 agttcagaaa attttaaaaa aaattaccgg aggtggctaa atgccgttgt tagatagctt
     3061 cacagtcgat catacccgga tggaagcgcc tgcagttcgg gtggcgaaaa caatgaacac
     3121 cccgcatggc gacgcaatca ccgtgttcga tctgcgcttc tgcgtgccga acaaagaagt
     3181 gatgccagaa agagggatcc ataccctgga gcacctgttt gctggtttta tgcgtaacca
     3241 tcttaacggt aagggtgtag agattatcga tatctcgcca atgggctgcc gcaccggttt
     3301 ttatatgagt ctgattggta cgccagatga gcagcgtgtt gctgatgcct ggaaagcggc
     3361 aatggaagac gtgctgaaag tgcaggatca gaatcagatc ccggaactga atgtctacca
     3421 gtgtggcact taccagatgc actcgttgca ggaagcgcag gatattgcgc gtagcattct
     3481 ggaacgtgac gtacgcatca acagcaacga agaactggca ctgccgaaag agaagttgca
     3541 ggaactgcac atctagtcag taaactatct tcacaattaa ttgaaaaaag ccagttcaaa
     3601 tgaactggct tagttgtact tagtgcgcac cgcctccgcc gccacctgcg ccaaatggcg
     3661 gtttagcaaa ccacaccagc cccagcagta cgaggaatat cccggctgac atccagaaga
     3721 tctcattggc ggaaataatc agcccctgat tggtgatctg ctgggcaatc cagcctgatg
     3781 cctgctgttg cgtcatccca agcccttcca gttgactgta catcgcctgg gcattcgggt
     3841 tgaacgggtt taccgactca gtcaactgcg catggtgcat cgactcgcgg ttggtccaca
     3901 tggtcgtggt tatcgacgtg ccgatagacc ccgccagcgt tcgcgtaaag ttagagaggc
     3961 tcgatgccgc cgccagtcgt tccggtggca aaccagacag cgtaatggtg gtcagcggca
     4021 taaagaagca ggccaccgca aacccctgga taaactgcgg ccaggccgac gcgccaaaat
     4081 ccatacctgg ttcaaaggta taggcacgcc agtagaagca gacggcatac ataataaagc
     4141 tgaaggttac cagccgccgc atatccagtt tatgcgcgaa gcggccgata atcggcgaca
     4201 ggatcaccgg aataatccct accggcgcag aggccaaacc tgcccaggtc gccgtgtaac
     4261 cgtagacctc ctgcaacaac tgcggcagca gaacaatagc gccgaagtag agcatatacg
     4321 cgaggctgat acacaagcag ccgatggtga agttgcgcga cttaaacaac gacagatcga
     4381 ctatcgggtt atcgtcggtc agctcccaga caatcaggaa gcagatagcc accaccgcca
     4441 ccacggtaag aatgataatt tcctgtgatg aaaaccagtc cagctcttta ccgcggtcga
     4501 gcataatctg caggctgccg ataccaataa ccagcagtgc cagccccacg gcatcaatcc
     4561 gccgccgttc ggtgcgggtt tcacgtccgc gcagagtttg cagtgtcatc aacaccaccg
     4621 ccacgccaat cggcacgttg atgaagaata tccagcccca gtggtaatta tcgctgatat
     4681 aaccgccgag gatcgggccg caaattggcg cgacaatcac cgtcatcgac cacaacgcca
     4741 gcgcgatcga gcgtttggct ggcgggtagt tattcagcaa tagactttgc gaaagcggga
     4801 tcaacggccc ggcgacaatc ccctgaatca cgcggaagaa gatcagcata ttcaggctgc
     4861 tggagacacc acacgcccac gacgcaatag caaaggcgat ggtggaccaa aggaacagtt
     4921 tcacttcccc gacgcgcttt gccagccagc cggtaagcgg gatcgagatg gcattcgcca
     4981 ccccgaaaga agtgattacc cacgttccct ggctgagcga tgagcccaga ttcccggcga
     5041 tagtggggat cgccacgtta gcaatggtgg agtccagcac ctgcatgaat gtcgccagtg
     5101 acagcgcaat cgtcataatg accagttgcg cgccttccag cggtttttgc tgttgcatca
     5161 cacgcacctc tggattagcc agcgttagct tttacgatat cgtcgatcag tttattgaca
     5221 ggtgccaggc tgatttcacg cgcggtactt accgcgaccg gtgttgaacg tactttattt
     5281 gccagtacct gaccgtcacg gttagtggta ttgacgctca ccagcgtgga caaaccaatg
     5341 cgcagcgggt attgctccag ctgtttctgg tccagttcga tacgcacagg tagacgctga
     5401 acgactttga tccagttacc ggtcgcattt tgcgccggaa gcagtgagaa cgcgctacct
     5461 gtgcccatat ccagaccaac cactttaccg gtgtatttca catcatcgcc gtaaatatcg
     5521 gtggtgatgg tgaccggctg accgatgcgc atattggcaa tctgcgtctc tttaaagttg
     5581 gcatccaccc acatattggt ggctggaacg accgccatca gcggcgtcgt tgggctaatt
     5641 tgcgcacccg gttgcacggc acgacgggaa acataaccag tcatcggact gacaatacgg
     5701 gtacgctcca gcgccagcca ggcgttacgt acttcggtgg cagcctgttg cacggcaggc
     5761 tgatcttcca gtttagtccc cagaatcatc gcctgattgg cattgtattg ttgaatagcg
     5821 acgtccagtt gcgcctgggc actggtgacg gcgtcgcggg cgtgttgcag ctcttcgaga
     5881 ccaatcaggt tggcattgcc cagcggcaca cggcggttgt agtcgctttg tgctttcgcg
     5941 agggcgattt tctgcacctc aatattcgcc tgcaactgct tgctgttaat catcagctgg
     6001 tgggtttggc gaacgctgga agccagtgca gttttggctt tttcaaacgc ctggcgagca
     6061 tctgtcgggt cgagagtgac cagaacatcg ccttctttta caaaatcggt gttatcggcc
     6121 cagactttcg tcacgctgcc agacacctga gacataattt gaatctgatt ccctgccacg
     6181 tatgcgtcat cggtttcttc gaaatgacgc agtaccaaaa accaataaat ccctatcgct
     6241 acggcaataa ttataaagag caaggtgaga aggaggagca gacgcttacg tttgccgctc
     6301 ttctttaccg gttgctgcgg ggtttgagtc tccgcatttg cgctcatatt gttctccacg
     6361 atcttcttaa ctcatcggct gagccgacct gcttgccaaa aaggccagca tgttgcgatg
     6421 ctggccagtc attttttctt ttataaatct ggatttttga gcgagatgac gcgttagctc
     6481 atcgcttcga gaaccacacc gtcttgttcc atctggtcga gacgggagag caatttgcgg
     6541 gtgatttgct cgagctgatc tttttctgtt gtgctgagcg cggaccagag ttgatgcagg
     6601 cagttatgct gcggtggtaa aacctcgcgc aaaaactcgt gacctttttc cgttaattgc
     6661 agatgcaggc agcggcgatc gttatcgctt tcacgacgtt cgatccaacc gcgtttttcc
     6721 agttcatcgg caatacgcgt cgcgttggta cgggatgatc caagagcaca acttaattca
     6781 gaaggctgaa tactgtggtt ttcctgagac tccagcgtaa tcaacgccat aaacaacgtc
     6841 tcgttaatcc cctgagcctt cagcatttta ttgcggttct ccagcagctt gctttgcatg
     6901 tgcatgcaaa gacgagtcag aaggatctcc tgataaggaa aatcttcgtg gcggctggcg
     6961 cgaaatttta gcatttgttc aatgggcgta aacgaactat ccatttgggt atgacctcat
     7021 taatttcagc cgatatagta acgacagtga caaataaagt aaatgtattg ttttagaaaa
     7081 atgattcttg tggattatat aatcgccatc actttccaga cgagcccgta ggtcagcgca
     7141 ctaagcagtg ttgggatgat aatgctgcgt gttttataga aactggcacc cagtaccgcg
     7201 aagccgacca gcgtgggcac gaaacggcgt gtatcgtgca tcacttctgg tgcggtagag
     7261 acaaccagca gagcgcatat cgaggcgatg ccaatggtgt cgagcaaaat acctaccgcg
     7321 ccacgtttgg ttgggcgggc attacccaca cgcaggcgca gcggcaaata gcggaagcaa
     7381 taattcgccg cgccaaccag taacccaagc agcagaacct catagctcat cgggcgctcc
     7441 ttgccagaat gcctggatta acgcagtgag gcagccacag acaatgcctg ccagaatggc
     7501 gacgggaata gaaaatagcg ttacgcctgc aagggcacca actaacgctg cggtaacgca
     7561 aagagattgt ttgcgctgga aagaggcgag caggaaactc ataaagagtg ccggaagcat
     7621 aaaacctaat gctgcttcaa cggcgggata accttgcagc aagccgctgc cggagaatgc
     7681 ccctattacc gtaccaaata cccacgatga ccatgaactg aaggcaatgc cgatcatcca
     7741 gttctcgctc cagcggcgat tattgcgtac cagttttgcg gttgcggcgg caaaaacctc
     7801 atccgtcagg ccaaacgccc acagggcggt tttcgatttt tgcagacgct gaataatacg
     7861 gctacgcagt gacgggccat acaacacatg gcgaacatcc attgccatga cggtcagtgc
     7921 agcaacccac aaactactcc cggctgccag catcgcggta atgacgaact ggctcgcgcc
     7981 tgcataaatg atgcaggaga aaaaaacgct ttcgagagga gagaatccca gacgggtcgc
     8041 attcagaccg aacgcaaagg ccaccggaat ataactaata acaatcggta aactgtcttt
     8101 gcatccttcc atgaaggtcg ccgaaccagg agcaggctgt ggagtagggc tttccataga
     8161 gtgtacgctt aacgattgtt aatattataa atagactgaa tgaatatctt aaccttatca
     8221 gactgatggg cttcttaaca cccttataag tgtaaagcca cgaaaacggt tgctgattgc
     8281 ttaatttgcg gcttcctgac gatgaaaacc tcgccaccag accagtaagt taaccagggc
     8341 aatcgtcgcg ccagccagac aaacgccagc ccaaccgcca tgttgccagg ctgaggctga
     8401 aattagcgaa ccggcggcac cgccaataaa gtagctagtc atgtaacctg cggtcaggcg
     8461 attacgcgca tcaggatgta tccgataaat taccgtctgg ttagtgatat gcacgccctg
     8521 cacggtgaga tccagcacca ggattccgat aatcaacgcc agtacggaag tgtgaccaaa
     8581 ccagatcgcc agccatgaaa gtaatagcag cagcagaccg aaagttgtgg tgtggtgcga
     8641 tttgccctta tcggcaaaac cgcccgccgg acgagcgccc aacgctccgg cagctcccgc
     8701 aagtccaaac agaccaatta caccatcgct gtagttaaaa ggtggagcgg caagcaaaaa
     8761 ggccattgag gtccagagaa tgctgaaatt ggcaaaggtc aggcagccca gcaacgcgcg
     8821 ggtacgcaga attttatcgc tgataaacat actgaaaacg gaacccaaca actgtgggta
     8881 gttgaggtgg gtttctgatt tcatttgtgg cagaccacgc cataatgcca gcgccatcag
     8941 tgccattaac accgaagcaa cccaaaagac ggtgcgccag ccgccgagat tcgccagcaa
     9001 tccggcaact gtccgtgcca gcaagatccc caacagcaga ccgctcataa tagtgccaac
     9061 cactttgccg cgtttgtccg gtgaagccag cgtcgctgcc agcggaacca gaatttgtgc
     9121 cacgacagag aataaaccgg ttaatgccgt accgaggatc atcatcgcca gcgactgact
     9181 gctggcagtg attaacatgc cgcctgccgc cagtaaggtc atcgagacaa tcaggcggcg
     9241 gcgttcaaac atatcaccga ggggaacaag aaacagcaga cctgctgcat agcccaattg
     9301 cgcggcggta acaataaagc ctgccgaact ggcggaaagg gaaaagttac gcgcgatggt
     9361 gtcgagcaat ggctgagcgt aatagttact ggcgaccgcc agaccggtgg cgatagacat
     9421 cagcacgatc agcgccgggc taagctcatg attaggttta gtcattattt ttgttcatca
     9481 ggtgatgatt aagctggaaa tcaataatag cggatgttga tgaatatggg atgaaagata
     9541 gcttgttgaa atgtgcgatg tggtttgtag gcatgataag acgcgccagc gtcgcatcag
     9601 gcattgtgca cagaacggcg gatgcggcga aaacgcctta tccgccatac ggaatgagtg
     9661 tattttgtag gcatgataag acgcgtcagc gtcgcatcag gcattgtgta cagagcggcg
     9721 gatgaggcaa aaacgcctta tccgcctgaa taaaaattac ttctgcgctg ccagcgcctc
     9781 attcacccag ccatcgaact gctgctggtg ggctttgatc cagccatcaa cgtggccctg
     9841 aatatcgcct tctgaggctt tgccgtcatg cataatggcg ttctgggcgt taatatctgc
     9901 cactggcaac tgcataatgg caaacagttt cgctgctgcc gggtttttct cggcccaggc
     9961 tttgttggca acgatatgca tggtgctgac cgggaagcca taattcgcac cattcggcag
    10021 tttggtatcg gcgtttttat cgcccggcag tgcggagaac ggcacctgca accagacgac
    10081 atctttgccc ggcttcagtt cgttactcac ccagtacggc gtccaggtgt agtaaaacac
    10141 cggtttgccc tctttgtagc gactgatggt gtcggccatc atcgccgcgt agttcccctg
    10201 attatgcgtc acggtatggg tcagttcata cgcggcaagc tggtggttga tcgcaccttc
    10261 gcagccccag ccagggttac aaccggttaa atccgctttt ccgtcgccgt tggtatcgaa
    10321 cagtttggcg atcttcggat ctttcagttg tgcgatgttg gtgattttgt actggtcggc
    10381 ggttttctta tcgatcaggt aaccctgtgc cgcgccgtta acaaataccc cttcacgata
    10441 aaatttctta tcgccaccgg cagcttcgta catgttgtca tgcagtggcg tccagttcac
    10501 ggcggtgaag gttgcatcgc cggaagcaag cgaggtgtag ccaacgttgt aatccacttc
    10561 gctgggtttg ttgacggtat aacctaattt ctccagcgca cgactgacca gcagcgtctg
    10621 gaaggtttct tcggtgatgg tgctttgaac tggattaaca gtaatacctt tgcccggcag
    10681 atcggcagca aaagtttgtg tagagataag cgtggcaaac gctgtcgcaa aaagtacgct
    10741 atgtcgcatt gttattcctt tttttggcag ggtgataatg cccggcgtca ccgggcaagt
    10801 gcagagttac ttaatgaatg ggcgggtcag cagaccaaca gggccagtgg tgtaccagcg
    10861 acggttgccg cgactgcgtg agtcgcgccc aacggcctgc gtcaggcgat cgaggataat
    10921 ggcgaggatc acaatcccga cgccgccaac ggtggcaagc cccatatcca gacgaccgat
    10981 accgcgaagt accatctgac ccagcccgcc gacggcaatc atcgaggcaa tgaccaccat
    11041 agaaagggcc agcatcagcg tctggttaac gcccgccata atggtcggca tcgccagcgg
    11101 taactgaact ttgaacagca tctggcgcgg gctggcaccg aatgagcgcg aggcttcaat
    11161 cagatccgcc ggaacctggt taatccccag aatggtcaga cggataatcg gcggcagagc
    11221 aaagatgatc gtcaccacta cgcccggcac gttaccgata ccaaatagca tgacgattgg
    11281 caccagataa acaaacgctg gcgtggtctg catggcatca agcagtggac gaataatttt
    11341 cgccgctcgc ggacttctcg ccagccagat ccccaacggc aaaccgatga cgatacagaa
    11401 cagcagggcg gttaacacca gcgccagagt aaccattgcc tgcgaccagg caccgattgc
    11461 gccgatggca atcagcgaaa ccagcgtcgc cacgcccatt ccgaccccgg aaatctgcca
    11521 ggcgatgaga gcgaaaacga taatcgccac cggtgcgggc atacccagca gcaactgctg
    11581 gaaaccgttg aggatataat caaccggaac gcgcacgccc tggaagacgg gacggaaatg
    11641 ggtaacaacc cagtcgatcc cttcagtgac ccaactgtcg agcgggatca gcgttttatg
    11701 gaacggatcg agaatattaa aatgctcgac gtttggcgca ggcgtactgg tcagccagtc
    11761 agcaccaccg ccgtcagtcg gtgcagtcgc cggtgtaccc caggcgtctg cggattgcgc
    11821 ggcactgtcc gccgctggcg tggtatccca cggattattt tgatcagcca ttatttaccc
    11881 cctcacgatc taaagcgcgc agcagcattc ctttcgaaat gatgccgaca tactgttggt
    11941 cctcgtcgac cacgggcacc gcacagggtg cctgtccgac atgagagagc aactcgctaa
    12001 gaggcgtttg tgcatcgact gctaacggcg catcaatcag cgccgcatca agaccttgct
    12061 gctgcgttaa cgcggtttta agcgaatcga tggagactgc gccgacaaac ttattaccgc
    12121 gttcgataac gtagccatat tcgcgatctt catcctgcaa taatttcagt gccgaacgtg
    12181 ggccgaagcc aggggtttta cgaattaagc cattcggtgt ccggcgggca atatctttcg
    12241 cactgaatac ctgactaata tcaacgccac ggaagaaggt acggacataa tcattcgccg
    12301 gattattgag aatttcatcc ggtgtgccga cctgtaccac ttcaccattt tgcataatgg
    12361 caattcggtc gccaatacgc atagcttcat cgagatcgtg ggaaataaag acaatggtgc
    12421 gctgatgttt cgcctgtaat tttaccagct catcctgcat ctcggtgcga attaatggat
    12481 cgagcgccga gaaggcttcg tccattaata atatatccgg attaatcgct aacgcgcggg
    12541 ctaatcccac acgttgacgc atcccgccag agagttcatc cgggtagctg tgggcataat
    12601 tttccagccc gacctgacgc agtgcatcaa gggctttttc ccggcgttct tcggcattaa
    12661 ttccggccaa ttccataccg aacgcagtat tgtccagcac ggtcatatgc ggcattaagg
    12721 caaaggactg gaagaccatc gcaatctttt ttctgcgcac ctcacggagt tcggcatcgg
    12781 atattttggc aatatccaca ccatcaatca gcacttgccc gcgggtgggt tcaatcaggc
    12841 gattgagaag gcgtaccatt gtggatttac ccgagccgga taatcccatg atgacaaata
    12901 tctcgccttc ttcaatggcc agactggcgt cttttacgcc aagcgatagc ccagtttttt
    12961 ccagaatttg ttctttcgaa agtccttgtt cgatatattt gaacgctcg
//
LOCUS       DAFNBJ010000134        12928 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000134 DAFNBJ010000000
VERSION     DAFNBJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.134"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            455..1663
                     /gene="trbA"
                     /locus_tag="L2X49_003689"
     CDS             455..1663
                     /gene="trbA"
                     /locus_tag="L2X49_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HBN4798710.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYTSCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPKKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            1682..2752
                     /gene="trbB"
                     /locus_tag="L2X49_003690"
     CDS             1682..2752
                     /gene="trbB"
                     /locus_tag="L2X49_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HBN4798711.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVLEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            2745..5036
                     /locus_tag="L2X49_003691"
     CDS             2745..5036
                     /locus_tag="L2X49_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HBN4798712.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     KTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELHPGEFFS
                     IFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(5073..7772)
                     /gene="nikB"
                     /locus_tag="L2X49_003692"
     CDS             complement(5073..7772)
                     /gene="nikB"
                     /locus_tag="L2X49_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HBN4798713.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVRHTLKSLGLGEHQYVSAVHTD
                     TDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(7783..8115)
                     /gene="nikA"
                     /locus_tag="L2X49_003693"
     CDS             complement(7783..8115)
                     /gene="nikA"
                     /locus_tag="L2X49_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HBN4798714.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            8349..8684
                     /locus_tag="L2X49_003694"
     CDS             8349..8684
                     /locus_tag="L2X49_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HBN4798715.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(8770..9618)
                     /locus_tag="L2X49_003695"
     CDS             complement(8770..9618)
                     /locus_tag="L2X49_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBN4798716.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRRMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNTSKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKRIIIENMLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            9968..10165
                     /locus_tag="L2X49_003696"
     CDS             9968..10165
                     /locus_tag="L2X49_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798717.1"
                     /translation="MQGAPHPARLKKNDGTTMACGGHWHTTPAQVKTEKIARHLQTKK
                     VKFLHFFTLIYRADLAIVMIK"
     gene            10199..10450
                     /locus_tag="L2X49_003697"
     CDS             10199..10450
                     /locus_tag="L2X49_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4798718.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(10481..10675)
                     /locus_tag="L2X49_003698"
     CDS             complement(10481..10675)
                     /locus_tag="L2X49_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798719.1"
                     /translation="MTLAERLIQKGFDEGFKEGFKKGALEVAREAACRLRDMGWTSER
                     IQEATGLSGEELKKLFPDEQ"
     gene            complement(10738..11628)
                     /locus_tag="L2X49_003699"
     CDS             complement(10738..11628)
                     /locus_tag="L2X49_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBN4798720.1"
                     /translation="MSKKKTTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSEAELKKIIH"
     gene            complement(11693..11959)
                     /locus_tag="L2X49_003700"
     CDS             complement(11693..11959)
                     /locus_tag="L2X49_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798721.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(12052..12486)
                     /locus_tag="L2X49_003701"
     CDS             complement(12052..12486)
                     /locus_tag="L2X49_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HBN4798722.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
BASE COUNT         3194 a         3410 c         3226 g         3098 t
ORIGIN      
        1 gtactggatt cctgttataa gagcggagaa aagcacccca taactatatg tagtgcaggt
       61 gagacaagta cctggtggga agacatatgg catgaagaca tgatagctca atcgaggaat
      121 aatgagtcct acttctggga tggtgacaaa cctcttattg atagccttca taaagaactt
      181 caaaaaaata acagattaaa gccaaacagt gagttagata tgccactaat tggttaaaca
      241 ccaattaaag attatctagc taaaacaaca tcgcacacgc atactgcaaa gcccttggtg
      301 agttaataga agaattacta agggcttata aaaaatatat acccccacat tggtgacaca
      361 ttaaaatatt ttttgttgat gtgttaccag cctgacccaa aaacaatttt taatctttcc
      421 gttcgattta aattccagaa atggagactt tgtcatgtct tacaatcgac agcctgtcgc
      481 agaagatcct atgcagatat ggggcgctgt tggtgtcctt cttatcctgc tgctattcgt
      541 catatggtta ttcttgccgg aagtggttta caccagctgc ctgattcttc ataccttatg
      601 gggactggtc gactggggac catttcataa ctatgcagca ccacgataca acctgcttgc
      661 catgaccggt aacaacgccg ccaacataag ctatagccag tgggttaacg tcatggaaca
      721 gaccataggt attctgtgga tgtacctctt acctgtgaca ctctggtgtt tgtgggaatg
      781 gtatcaacat ccgggacaaa gccgtttcac ccgcagacca gtagatatta cccgactgcc
      841 acacatcttt gcttcgttat cccctgctat cgcccctgta ctggcagatg gtgatccgaa
      901 aaaactcttt catggtggta aacgacctga acggcgggtt gctctcaccc cggaagcatt
      961 tgtcgaacag catactctca tcaccaatat gcagctcgat gttgcagctg caagacgatg
     1021 tttcatggcc cagttgggta aacctttaac gtcctggaaa gacatggcac cacatgaaaa
     1081 agcgctcttt gccatatttg gccttcagta tttcctggat gatcggaagg cagcattaaa
     1141 gctcatggat acactgaacc tctcatgcag aattaaaagc aaacgggaca gcgggaaatt
     1201 ctgtacccct gtttattcac tggccaaatc agctttccag agagtgatta aaagcaacgg
     1261 agcacagcaa tggctgaaac aacatcgcta cgtccgcagt gggctggtct ggctgtatgc
     1321 acatgacctg cggcttacgc cacccaactg gatttggctg aaaggcgtgg acagaacgct
     1381 gttttatgca ttacaccgcg ccaataccac gaaaggattt attgaaggcg ctggcgtggt
     1441 ggccgttgca cgtgcagagg ccgaagccat gcgttttggt cttccctgcc ctgaaccctg
     1501 tgttgatgaa gcggtggaag gcttacggcg ggatatgctc agtctcggtc ttatctggga
     1561 tgaaccacaa ccagatcgcg accggaaacg ccgcatcctg accaactggt ccctcaccga
     1621 cgacatcctg ccccgcacac ctgctacaga caacgaattc tgattatccg gagaactgaa
     1681 aatgacaatc gagtactttg caacacgcat tgcaaaacat atttccgtgc aggcaatctg
     1741 gccggatggc aggacagaaa ttattgccgt ccttccacgg gatgccctgt cagggctggt
     1801 aacacgaaat aatcaactga ttcatatagc ctttgacgcc atgggcaccc gtaacagaga
     1861 aaccgtcctg attgataaac aggagcataa cgctgaccag atacttacag ctattgattc
     1921 ctgcctgcgc cttgaatatc tgatggaacg ccgcttcagc agaacacctt tgttccgtgg
     1981 cataatcgcc agcgttgtgc tgttcgtaat ggcaacgatt ggcttctctc tgttccgcta
     2041 tgttgatcgt gtattctggg atgatacaac gcctgaagca gtacagacag caggtgagcc
     2101 acgactgcta ccccctcacc tgaatcacac tgtgccatta aatgaaggca ttcaactccc
     2161 ggttcccccg aaaaaagacg tccaggtcct ggagaaaaca atcacaagca aaaatccgga
     2221 agccgcagct gcgcgacata atctggctac tgttctgaag cggaacgccg atcggggcat
     2281 gtttaccatc aacctgtcat ccggtcatga acgaaccctg ttcgcatttc tcgatcctgc
     2341 ctgtcctaac tgcagacttc tggagccggc cttaaaacgc ctggccagcg acttcaatgt
     2401 cgttatctac ccggtttcag ttattggtgg agaggaatcc accgaccgtg tggcaccact
     2461 gctgtgtgaa aaagatgctc agaaacgtgc tgccggctgg caccgactgt attccgcaga
     2521 taacggaatg atgacgccat cagaagaaac aacgcctgca gatgaaacat gcctgaaagc
     2581 agccagagct gcgattgatg ttaacaatgt cgccttccgt aagttcggat ttgccggtac
     2641 cccatgggtg ctgtccgaca ccggctggca tctgcctaca ggaattctgc aggaaactgg
     2701 cacgcttaat ttattcctga aaaccaccga cagcgagtct ggccatgagt gaacatcgcg
     2761 ttaaccccga gcttttacac cgtacagcct ggggaaatcc tgtctggaat gcactccaga
     2821 gtctcaatat ctatggcttt tgcctggtgg caagtcttgt ggccagtttt atctggccac
     2881 ttgcattacc ggcatgtctg ttgttcacac tgataacaat gctggtattt agtctgcagc
     2941 gatggcgctg ccctcttcgt atgcccatga cgctggaatg tgctgacccg tcacaggaca
     3001 gaatgattaa aagaagtctt ttcagcttct ggccaaccct gttccagtat gaggtcattc
     3061 tggaatcacc ggccagcggc atattctatg ttggttatca acgcgtcagg gatattggca
     3121 gggagttatg gctgagtatg gacgatctga cccgccatat aatgtttttc gccaccaccg
     3181 gtggcggtaa aacggaaacc atctttgcct gggcgattaa cccactttgc tgggcaagag
     3241 gattcaccct tgtcgatggc aaggcacaga atgatacggc aagaacgatc tggtatctgg
     3301 ccagacgctt tggacgtgaa gacgatgtgg aagtcattaa cttcatgaac ggaggaaaat
     3361 cccgcagtga aatcattctg agtggtgaaa aaacgcgtcc tcagtcaaat acctggaatc
     3421 ccttctgtta cagcactgag gcattcacag cggagacaat gcagtcaatg ctgccccaga
     3481 acgtccaggg gggcgagtgg cagtcccggg ccattgccat gaataaggca ctggtatttg
     3541 gtactaaatt ctggtgtgtg cgcgaaggca aaacaatgtc gctgcagatg ctccgtgaac
     3601 atatgactct cgaagggatg gccaaacttt actgtcgtgg cctcgatgat cagtggccgg
     3661 aagaagctat cgcaccgtta cgtaactacc ttcaggacgt tcccggtttc gacctgtcgc
     3721 tggtcaggac accatcagcc tggacggaag aacccagaaa acaacatgct tatctctccg
     3781 ggcagttttc tgaaactttc agcacattca cggaagcatt cggggatatt tttgctgaag
     3841 attccggtga tatcgatatc cgtgacagca ttcacagcga caggattctg atggtaatga
     3901 tccccgctct ggatacatca gcgcatacaa cgtctgctct tggacgaatg tttatcaccc
     3961 agaaaagtat gattctcgcc cgtgatttgg gctaccgcct cgaaggaact gattccgatg
     4021 cactggaagt caaaaaatat aaggggcgtt tcccttatct gtgtttcctt gatgaagtgg
     4081 gggcatacta tacggaccgt attgctgtgg aagccactca ggtgcgttca ctggattttg
     4141 ctctgatttt gatggcacag gaccaggaac gtatagaagg acagacaaca gccacgaata
     4201 cggcaacact gatgcagaac acggggacaa aatttgccgg caggattgtc agcgagggca
     4261 gtacggccaa aacgctgaaa agcgccgccg gtgaagaagc cagggcgagg atgaacaacc
     4321 tgcagcgtca ggacggcata tttggtgaat catggattga cagcccacaa atcagcatcc
     4381 tgatggaaag taagatagat gttcaggagt tgattgaact tcatcctggc gagttctttt
     4441 ctattttccg gggagaaacc gtaccatcag catcattctt catccctgac aatgagaaaa
     4501 gctgcagtag cgatcccgtt gttataaaca ggtatatcag cgtggatgca cctcgcctgg
     4561 accgtctccg ccgtctggtt ccccgtacag ctcaacgtcg aataccgtca ccggaaaatg
     4621 tcagtgccat cattggcgtc ctgactgcaa aaccctccag aaaaagacga aaaattcgca
     4681 cagagccgca cacgatcgtg gatacattcc agcaacgcat tgcaggacgt caggcggcta
     4741 tggcaatgct ggaagagtac gacacagata tcaacgcacg tgaaagtgcc ttgtgggaaa
     4801 cagcggtgaa tacgcttaaa acgaccacac gggaagagcg acgaattcgc tatatcacgc
     4861 tgaacagacc agaactacct gaaacaaaag aggaaaatca gatatcagtg agagcagaaa
     4921 gagcaggtat aaacctgtta acgcttccac aggacaataa ccacccgaca gggcgtcctg
     4981 taaacgggtt tcatcataaa aaaaccaacc gtccggactg ggacggaatg tactgaacag
     5041 tggccgcgcc ggattctggc gcggcctttt ttttaacctg gagaaggcgg ttcccagcgt
     5101 ttatcctgct ccggcggagg cagtgatgcg tcatcgttgc tgttcgccgc gatataatcg
     5161 cggtagtagc gttcacttcc ttcacggtga tgatccacgt ccggtcgcgt aatcctgtag
     5221 tcttcgtgac cggtacggcc tgtcacatta tgccaggcaa ccatttcagc aaagacctgg
     5281 ttaaattgtt tgtcgtttcc ttctggttca aaacccatgc gtggatcacg cccgaataat
     5341 accggtgcaa tcaaatccag tttatctgcc agcgcattgc ggtcatggtg attacggaaa
     5401 acaaccctgt caccataatc cgtacagaca aactctcccg tccgacggtc acggaaacga
     5461 acactcccgt tcttctgcag acgggcctcg agatcaccgg tattaccgta taccggcgtt
     5521 ccgcccggtt cacaaagcac gacacagcgg tctgtcgtcg ttgtgcgtga cctgtcttta
     5581 cggcgatcgc gataatccca gcctctcagc tgcgatacag cagccctgtc tccctgagca
     5641 gcctgaattt cgacccactg acggtaagag ggtggatacc acttcccgtc agcaataagt
     5701 ttctgtcgct catccctgat gtcttctttc agcctgatca atgcctgcat tcgctgaact
     5761 tctgcaatgt gataatgcag cttacgtaat gccggatcat cgtactgcgc acgaatgtgt
     5821 gacttccgca gacggcatgc ctgatgaatt tcccgacaac gttccccata acgcagatct
     5881 ggtttacgcc attgttcacg ccatgccaga tagcgggcac gcaggtcagc acgggcctca
     5941 gctctggctt cacgtcgctg ccggcgcagc attgggtcac gtttactgct gaccccgtac
     6001 ctgtcactga cagccagctc cgggttatag cgactttcag gttgcaccct gtcaaacaaa
     6061 tctgctgggg cactcacaaa aggtccggcc tgtggctctg cacgccccag tgtcagatca
     6121 ggatgaatgc tgctggcctt caccggcgtt tgctcatgat taaaggcatc aacgacaaca
     6181 agcccgtgat gttgtttctg tagcatcagt ccctgttgtg caaacagctc atgacaacgc
     6241 tgccaggtaa actcaccctg tgtgatcatt tcttcaatct gagggctaca atggctgatg
     6301 ctgtatcccc acagactttc tttgtcagaa aacagttcgc gtttgatagc cccctgcggc
     6361 cccgtgcctg tacgcatacg gtgccattct ttatcggtcg caggacagct ctccatacga
     6421 cgaccacgga agcgttctgt tggcgtaacc tggcggaaaa tatctgttgg tacaggctgc
     6481 cagccaccat cgagttgatt cacattgtcg agcgtcagca gcgaccatac gctgtcagca
     6541 cgaacgggag tctggttatg atcataaccg tcacagataa ccaggtgacc atgctgaaca
     6601 cgcatccata atcccgcttc agccagtgtg cggtgaatag cctggcagtt ttccagccgt
     6661 ccttcccgtg ccatttccgg cagacgttct ccaagatgac gacaggcata ctgctgcaga
     6721 ctttctgttt cagcgatttt ttccgggcga acatcagcgg caatgaagtc cggattatag
     6781 cgtcctggtg cttccacctg gctgaaaatg tcttttggta ctggcgtgta gtcacccatt
     6841 tttttcatga gtttttctgc acaccaggag ggaccaaacg agtcaagctg tacaccttcc
     6901 ctgttgcgat cccagccatc catcaccaga aattttccgt ccatctgaga caggtacaga
     6961 ccatcttccg caagacgacg atgcagggat aaccagtcat ttacaggttc actgcgtaaa
     7021 ccagcgaccg ctgtctgcgc gacatactcg cgaaaagttt gtttttttcc gcgtgtccaa
     7081 gcgttctggc gatcacgttc aacggcagtt ttgcgaacga tacgatttcc cggcgcatgg
     7141 acccagcaac cgttatccgg ggcaaaacca tgcttcagtt caagctcacg gcaggcacgg
     7201 ctgagttttt cctgactcca tgaaaggcaa ttgagataac ctgtaaccgg atgtacccgg
     7261 ttgaccgcaa cgtgaacatg caggttatca gtatcggtat gtacggcaga aacatactga
     7321 tgttcaccca gcccgaggga tttcagggta tggcgtacac tgtcataaat ctgttccgga
     7381 cgcgggcttt catgggcctg ccaggaaaga atgtagtgaa aaacaggatc agtattatct
     7441 ttcgcatagt gtgcctgctg tgcaatgtac tccatatctg cagcagcagt ttcaagcgat
     7501 gtacagttgt gaaaacaggt cacaccgtaa aagttaaccc attcgcagcc gtccttcatg
     7561 acatcaacca gtgccacgaa tgactcattt cgaaggcgtg tcgcataatc aacaagacga
     7621 ctgaaacggc tccggtgcgg ttgttcagcc tgcgaagaag aagataaatt cagttcctca
     7681 tcggtcatgt catcccggac cgagacgtag gacaccaggt cttcgaacga agacttgccg
     7741 tcccttctct ttttcggaat gactgcattc accggagaat cctcaacctt ccattaactg
     7801 tttacgcaat gtatttgtaa gttcggttat ggcaacaagc acttctgcgt attccttgtc
     7861 accggttctt tttccctgaa caaacagatg cttctgcatc cttcccagtc tcattaactc
     7921 cttcagaaaa gcatcatctg tacgggagtt aattcgctta ttcagtgctg catttcggat
     7981 gtaagcagat acagtaagtc cggaatcttc agcttttttc ctgatggttt catcctcaac
     8041 aggagagaat ctgagtgtcc tgacaactgt cttttgccgt acctcacttt tctttcttac
     8101 cgcactatca ctcacttcag gctccttacg gggtgtcggg gcgaagccct gaccagatgg
     8161 taattgtaat agcgtcgcgt gtgacggtat tacaattaca catcctgtcc cgtttttcag
     8221 gccattatag cctgttattt ttgtgcatca tagtcttatg cagacggcag aagcagggcg
     8281 cagccctgcc aggaggcagt ttttgatgat aaaatcagac ttcaacatga aacggagaga
     8341 aagtgccaat ggcttctaaa aaattttact cagatgatga catccagctg gcaaaagctg
     8401 ccttatcgga attgccggac ctcaccgctc aacggaaaac attgcgtgat tttcttgatg
     8461 ccattcgtga tgacatcatt gtcctggtaa ggacaaaggg ttatacactg gcagatgttc
     8521 gtgacacgct taaaaatgca gggtatgaag taggagagaa ggcacttcgt gacattattc
     8581 gggaagcaga atcgaaaaaa ccatcccgcc gttcttcctc taaaacagca cctaaaaaga
     8641 cagattcagg caggaaagat ggcatcgata tgaacaacaa ttgatgtgac aatgtcacat
     8701 cgtgttctga gattatgatg attacagaaa aaaaagcccc ggcataaata ccagggcttt
     8761 tctgtttaat cagactcgtg gcggcaatgc accaggataa tgccgtgcaa taatatcatt
     8821 cagtttttca acaaggtcag gacgcttaaa gacaatgtgt acggtccctt tctggtagcc
     8881 acgcaccgta aaatattccc cttctaacag cttaccgacg ttgtcgccgt tcccgagaaa
     8941 ttcaccatac tgcgccccga tgctgctccg gaagtcagga acgttgcgcc cctccagcag
     9001 ataaaaagga cgtaccaggt catccagtgc ctcccgtccg ctgcagtcca gcgtcacacg
     9061 cccgttactc cagcggtaca gcatgttttc aataatgatt ctcttgccca gacggcaggg
     9121 attattggtt ctgtaatccc atgacaattt ccggtagaca tcaatcagtc cctgttcaaa
     9181 cgtctgcatc ttgctggtat taagatggcg gaatgttgcc agtacattgt ccagggtgat
     9241 ttcaggacag gtgtcgctca tcagctggct gttccactca tcacgctggc atgaactcat
     9301 gaaggtgtac atgcccgtat ctgtcatcag gcggtcccag attttccggt cggcaagacg
     9361 ggtgatattc cttttcgctt cttccggctt atccatgcga cgaacggaat cagtcacgca
     9421 gtcttcaaac ccgtaaagaa aaatatcacc tgacgcatcg agcagaatcc tgcgcgctgt
     9481 cgccaggcat tccatcgcct gcatgaatag tgcaatacct tcgttacgct gcgcgatgat
     9541 gcggtcgata gccacagacg gaataacatc tgtcgtgccg gcagtattca ggtcagcagt
     9601 gggtaaagtc tgattcatca cattgtctcc tcatcgtcac gactgccggg tcagccaggt
     9661 catttcccgg aggagcgaag ctccttctgc cgtccgggtc ttagggtatt tccccgcagt
     9721 ggacctttcc gacgggggac gctcaccacg tcacggggca gttgtccggg ggcagacgca
     9781 aaagtttttg acgccagcgg caaaaagttt tgtggctggt acatctctcc cctggacgac
     9841 tgaaacggga tgtggtcgtg tatgtacccc cggaaagtcc gtctgcggag aacaggccgc
     9901 agtgcggcgg cagacaggca agagacagct gcgccatcag gcgcagtggc cttacacacc
     9961 gtaaggtgtg cagggagcgc cgcatccggc gcgactgaag aaaaacgacg gcacaacaat
    10021 ggcatgtggt ggtcactggc acaccactcc cgcacaggtg aaaacagaga aaatcgcacg
    10081 acatttacaa acaaaaaaag tgaaatttct tcactttttt acacttattt atcgcgctga
    10141 tttggcaatt gtgatgataa aataaagtga agttttttca ctttttcaaa ggtgttttat
    10201 gaccggctgg gaactgcgaa tctggcgaaa aagcatgcta tggtcacgcg aaaaagcggc
    10261 cagagaattt ggcgtgacgc agagaacatg gcacgcctgg gaaaatgcgg aacaggtgga
    10321 cgtcaccgtc tggaggacca cccaggcact ttcagtccgt gacctgctgc ctcacatgca
    10381 ggggatgcgc aaggcagata tcatcaggcg tctggaaaat gaactggggg aaacggcagg
    10441 gaatgtgtga gcgtgattca cagcctggca gaatgtcagg ctactgctca tcaggaaaca
    10501 gctttttcag ttcttcaccg gaaagtccgg tcgcctcctg aatccgttcc gacgtccagc
    10561 ccatatcccg cagccggcag gctgcttccc gcgccacttc aagcgcacct ttcttaaaac
    10621 cttctttaaa accttcgtcg aaacctttct gtataagacg ttctgcaagc gtcatactgt
    10681 tttctctgct cctgctgaca aaaaagcccg gcattttgcc gggctgtgat gctctcatca
    10741 gtgaatgatt tttttcagtt cagcttcgga aagtccggta gcctgcttaa tctgctccgg
    10801 cgtcatcccc attttctgaa gctggcacgc aatagccagg gctttttcaa gcgctccttc
    10861 ctggcgacct ttttgctcaa gacgttctgc gatagtcatc agtttatcct catgctgcgg
    10921 cagacgctca gccagcgccc gcataaattc cggtgataca gagtcccctg cgttaacgat
    10981 gtaatgaatc agaacacgca cctgctcgtc tgaaatttcc accatgacct gcgggagttt
    11041 gtccagcagc tccatcatgt cacgatggcg gatatgcttc atcagcagcg tcagtgcggc
    11101 catccggcga tggttcatga tttcattgtc atcaacgaca gtgatatcaa ccagcggaaa
    11161 cggctttgtg tatattttag ccgcaagtgc cggattctca aaacagtcca gccagttcat
    11221 gctgtacggg tacgggctgc gctcaccatg atagaacagc acaggaatca ccagtggcaa
    11281 cgttttgtgc tgctccagat gtctctgcat cgccgccaca ctgtaacgta acatgcggaa
    11341 aggcatgtac agattttcgg tgctctggtg ctcgatgagc gtataaacat agccgtcttc
    11401 acccccggtg gttttcacac tccagaggat atcgctggcg tactgatgca ggtccggctc
    11461 aacaaaggtg gcgggttcca gcttcagcgt ggacaggtcg cacaactgcc ggtactccgc
    11521 cggaagatgc agttccagaa aatctctggc gacgtcggga ttcgccagga acgaccggaa
    11581 cgcggcatca tgcggcgtgg gcgtcgtggt ggttttcttc ttactcacgg ttcctcctgg
    11641 tgagatgtct gaacgtatcc tgccgcctgc acaggcagac ggcaagggtt aatcagtggc
    11701 agacccgctc aatttcaagc gcgcggagca ggtggcccca ttcgctgact ttcatagcgg
    11761 ggatacgtac ccgcttctgc tcactgcagc gtctggcgat atgaccgctc atcgccagga
    11821 cgtccgggtt aatctcatcc tgtgccagct cctgacgtct gaagcgggta tggcggtgca
    11881 ggggtttatt ctcccgcagg cactcttcac gcagacgctt tacatactcg gcggttgcga
    11941 agataattga tggtctcatg gcataatctc catagatagt gattgagatg ccggacttcc
    12001 tggcggggaa gcgccggcgg agagaggcag ggtttatcgc cctgcctttt ttcatttcgg
    12061 acagcgacgc agtgccagcg cgagcgcttt catcttccag ccttccggcg tgcggtacat
    12121 caccttccag tagccgtcga tgaacacata atggaagcgc acctcttccg tgctttcagt
    12181 ggcaatagcc tccctgaaag aacggtactc acgcgcctca ctgtccatcc ggaaaccact
    12241 gtcacgtccg taataagccg tcacaccgtc caccgggttc tgaagcgtat gcccggcggg
    12301 tttgtcgcaa cggggaccga ggcaacggat atcaccgtgt tcaaccaggg cttcggcctg
    12361 ctgaccggtg gcatagtggg tatgcaggat gcgaccgttc caggtcggct caccgtgcag
    12421 gtggcagtaa acggtacgga acaccccgtc gcggcaaaaa acggaaatcg tgctgctgac
    12481 agtcatgaaa acgtctcctt ctgctgaatc aggtcgccgg gccgaccggt tgctccccgg
    12541 aggggcgaaa gccccttctg ccgtccactt cttcggggtt tctccccgca gtggaccttt
    12601 ccgatggggc gtccgtcacc acgtcacggg gcagctgtcc gggggcggac gcaaaatttt
    12661 ttgccgggtt tatggctaaa aattttgtgg acggtatatc ttcccctgga ctgctgaaac
    12721 gggatgtggt tactccagcc cccggaaagt ccacagcggg aaacaggccg caggggtggc
    12781 ggcggaaggg ttgactgcgc catcaggcgc agtggcctta cacaccgtca ggtgtgcagg
    12841 gagcgcaggt cacggcgcga cagaggcggc gcgaccggaa cggtcgctgc aagcctggtt
    12901 tttcagacat cggaccgtag cggcagcc
//
LOCUS       DAFNBJ010000135        12719 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000135 DAFNBJ010000000
VERSION     DAFNBJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.135"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(82..1167)
                     /gene="dmlA"
                     /locus_tag="L2X49_003702"
     CDS             complement(82..1167)
                     /gene="dmlA"
                     /locus_tag="L2X49_003702"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HBN4798723.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            1270..2193
                     /gene="dmlR"
                     /locus_tag="L2X49_003703"
     CDS             1270..2193
                     /gene="dmlR"
                     /locus_tag="L2X49_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HBN4798724.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2320..2958
                     /gene="leuE"
                     /locus_tag="L2X49_003704"
     CDS             2320..2958
                     /gene="leuE"
                     /locus_tag="L2X49_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HBN4798725.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            3131..3490
                     /gene="yeaR"
                     /locus_tag="L2X49_003705"
     CDS             3131..3490
                     /gene="yeaR"
                     /locus_tag="L2X49_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HBN4798726.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYFGYPDEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            3494..3676
                     /locus_tag="L2X49_003706"
     CDS             3494..3676
                     /locus_tag="L2X49_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HBN4798727.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            3824..4072
                     /locus_tag="L2X49_003707"
     CDS             3824..4072
                     /locus_tag="L2X49_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBN4798728.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(4131..4205)
                     /gene="yoaJ"
                     /locus_tag="L2X49_003708"
     CDS             complement(4131..4205)
                     /gene="yoaJ"
                     /locus_tag="L2X49_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBN4798729.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(4208..4306)
                     /locus_tag="L2X49_003709"
     CDS             complement(4208..4306)
                     /locus_tag="L2X49_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBN4798730.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(4339..5364)
                     /gene="dgcP"
                     /locus_tag="L2X49_003710"
     CDS             complement(4339..5364)
                     /gene="dgcP"
                     /locus_tag="L2X49_003710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBN4798731.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            5547..5801
                     /gene="yoaF"
                     /locus_tag="L2X49_003711"
     CDS             5547..5801
                     /gene="yoaF"
                     /locus_tag="L2X49_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBN4798732.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(5823..6170)
                     /locus_tag="L2X49_003712"
     CDS             complement(5823..6170)
                     /locus_tag="L2X49_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBN4798733.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6225..7406)
                     /gene="nimT"
                     /locus_tag="L2X49_003713"
     CDS             complement(6225..7406)
                     /gene="nimT"
                     /locus_tag="L2X49_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HBN4798734.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     FNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            7503..8324
                     /gene="nimR"
                     /locus_tag="L2X49_003714"
     CDS             7503..8324
                     /gene="nimR"
                     /locus_tag="L2X49_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HBN4798735.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTHRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(8281..8727)
                     /locus_tag="L2X49_003715"
     CDS             complement(8281..8727)
                     /locus_tag="L2X49_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBN4798736.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            8896..9000
                     /locus_tag="L2X49_003716"
     CDS             8896..9000
                     /locus_tag="L2X49_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798737.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(9001..9504)
                     /gene="yeaK"
                     /locus_tag="L2X49_003717"
     CDS             complement(9001..9504)
                     /gene="yeaK"
                     /locus_tag="L2X49_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBN4798738.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(9547..11037)
                     /locus_tag="L2X49_003718"
     CDS             complement(9547..11037)
                     /locus_tag="L2X49_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN4798739.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            complement(11218..12693)
                     /locus_tag="L2X49_003719"
     CDS             complement(11218..12693)
                     /locus_tag="L2X49_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN4798740.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         3450 a         2954 c         3076 g         3239 t
ORIGIN      
        1 tgaaatgacg caggttattt actgcaaaga acgaataaaa aaaccgcgtc agtgacgcgg
       61 ttcaaataaa ctggtttgac cttaacgcaa aataattttg caaatcgcgt cggcaacctg
      121 tggcgtggtg gcattgcctt tcatatcagg tgttttcggc ccgtgagcaa tcacttcttc
      181 aattgctgcc agaataccgt tatgcgcttg ctggaaacgc tcatcgccat tgccgagaaa
      241 atcgagcatc attgcaccgg cccaaatcgt ggcgataggg ttagcaatgt ttttcccgta
      301 gatatccggc gcggaaccgt ggactggctc gaagagcgac gggaaagtgc gttccggatt
      361 caggttggcg gatggggcaa tgccaatggt gccggtgcag gccgggccaa gatcggaaag
      421 gatatcgcca aataaattgg acgccaccac cacatcgaag cgttccggct gcatcacaaa
      481 acgcgcgcag agaatatcaa tatgctgctt gtcccagcgg atctccgggt aattctcggc
      541 cattgcttcc actcgctcat cccagtacgg catgctgatg gctaaaccgt ttgatttagt
      601 ggcagaagtt agcgtcttac gtgggcggct ttgcgcaagt tcgaaggcat aacgcaaaat
      661 gcgatcgaca ccacgacgcg taaataccga ttcctgaatg acgacttcat gctctgtacc
      721 ttcattcact ctaccgccga gcgaggaata ttcgccttcg gtgttttccc tgaccacgta
      781 aaaatcgatg tcgccaggct gttttcccgc cagcgggcag ggaacgccag gaaagagacg
      841 aaccgggcgc aggttgacgt actggtcgaa ttcacgacga aatttcagca gcgaacccca
      901 caacgaaata tggtcaggaa cggtgtccgg ccagccgacg gcaccaaaat agatggcgtc
      961 gaaacggcta agttgctcat gccagtcgtc cggcatcatt ttaccgtgat ggctgtaata
     1021 ctcgcagctc gcccactcca tttgctcaaa actcaaggcg aagccccagc gctcagcggc
     1081 agcctgtaac acgcgaatcc cttcaggaag gacttctttg ccaatcccgt ctcccgggat
     1141 cgcagcaata cgcatcgttt tcatcattgg gttctcactt cctgaccatc aaaattgaca
     1201 acatcaccat cctataattg actgaaaagg aattaatccc cgtaactggt gaaacataaa
     1261 acacgaatca tgaataattt accgctgctg aatgatttgc gcgtctttat gctggtggct
     1321 cgtcgggctg gttttgccgc cgtggcggaa gaactgggcg tttcaccggc gttcgtcagc
     1381 aagcgcatcg ccttgctgga gcaaacgcta aacgtggtgt tgttacaccg caccacccgc
     1441 cgcgtcacca ttaccgaaga aggagagcgg atttatgaat gggcgcagcg gattttgcag
     1501 gatgtcggtc agatgatgga cgaactttct gacgtgcgtc aggtgccgca ggggatgctg
     1561 cgtattatca gcagctttgg ttttggacga caggtggtgg ctccggcgtt atcagcgctg
     1621 gctaaggcgt atccgcaact ggaattacgt tttgatgtgg aagaccggtt ggtcgatttg
     1681 gtcaatgaag gtgttgatct cgatattcgc attggcgatg acatcgcccc taatttaatc
     1741 gcccgcaaac tggcgactaa ttaccgcatt ctctgcgcgt cgccggaatt tattgcgcaa
     1801 catggcgcgc cgaaacattt aaccgatctt tccgcattgc cgtgcctggt cattaaagag
     1861 cgcgatcatc cttttggtgt ctggcaatta cgcaataaag aaggccacca tgcgattaaa
     1921 gtcaccggcc cactgtcgtc taatcatgga gaaattgtcc atcagtggtg tctcgatggg
     1981 caggggattg ctctgcgctc ctggtgggat gttagcgaaa acattgccag tggtcattta
     2041 gtgcaagtgc taccggaata ttaccagcca gcgaacgtct gggccgttta tgtttcaagg
     2101 ctagcgacgt cagcgaaagt gcggataacg gtagaatttt tacgccagta ttttgccgag
     2161 cactacccga atttttcact ggagcatgcc tgattaatga ttcaattatc gggttgatat
     2221 caggttaaaa cctgattttc tcctttctaa gccgctacag attggttagc atattcacct
     2281 ttaatcgcgc atgatcgaaa gataattaaa gaggttaatg tgttcgctga atacggggtt
     2341 ctgaattact ggacctatct ggttggggcc atttttattg tgttggtgcc agggccaaat
     2401 actctgtttg tactcaaaaa tagcgtcagt agcggtatga aaggcggtta tcttgcggcc
     2461 tgcggtgtat ttattggcga tgcggtattg atgtttctgg catgggctgg agtggcgaca
     2521 ttaattaaga ccaccccgat attattcaac attgtacgtt atcttggtgc gttttatttg
     2581 ctctatctgg ggagtaaaat tctttacgcg accctgaagg gtaaaaatag cgaggccaaa
     2641 tccgatgagc cccaatacgg tgctattttt aaacgcgcgt taattttgag cctgactaat
     2701 ccgaaagcca ttttgttcta tgtgtcgttt ttcgtacagt ttatcgatgt taatgcccca
     2761 catacgggaa tttcattctt tattctggcg acgacgctgg aactggtgag tttctgctat
     2821 ttgagcttcc tgattatttc tggtgctttt gtcacgcagt acatacgtac caaaaagaaa
     2881 ctggctaaag tgggaaactc actgattggt ttgatgttcg tgggtttcgc tgcccgactg
     2941 gcgacgctgc aatcctgata cattcagccc gcgttgtcgc gggcttccca cttgtaatcc
     3001 tccctgattt ttctctgata tggtgctaaa aagtaaccaa taaatggtat ttaaaatgca
     3061 aattatcagg cgtaccctga aacggctgga ataaaccgtt ttcagcgcat tcaccgaagg
     3121 agggaaaagg atgctgcaaa tcccacagaa ttatattcat acgcgctcaa cgcctttctg
     3181 gaataaacaa actgcgcctg ccggaatatt cgaacgtcat cttgataaag gaacgcgccc
     3241 gggggtttac ccacgccttt ccgttatgca tggggcggtc aaatatttcg gctaccctga
     3301 tgaacacagt gcagagcctg atcaggtgat ccttatcgaa gcggggcagt ttgcggtgtt
     3361 ccctccagaa aagtggcaca acattgaagc catgactgac gatacttatt tcaacattga
     3421 cttcttcgtg gctcctgaag tcctgatgga aggtgcgcaa caacggaaag tcattcataa
     3481 cgggaaatga atcatgggca aagcaacgta taccgtgacc gtcaccaata acagcaatgg
     3541 cgtttctgtc gattatgaaa cagagacgcc gatgactttg ctggtgccag aagtggcggc
     3601 tgaagtgata aaagatctgg tgaataccgt acgttcttat gacacggaaa acgaacatga
     3661 tgtttgtggt tggtaattat cactgatcgt tgatggcgca atttctgcgc catcgcaaaa
     3721 aaaacctccg tgatgctcgt cacaaatctt gccctcccga tgttgtgcta tgctttttat
     3781 cagcgactaa cccgctaatg caaaacagat aagtgaggaa acaatgggca ttctgtcatg
     3841 gattattttt gggcttattg ccggtattct ggcgaagtgg atcatgccag gtaaagatgg
     3901 cggtggattc tttatgacta tcctgctggg gatagtcggt gccgtagtcg gcggatggat
     3961 cagcacgctg tttggctttg gtaaagtcga tggcttcaat tttggcagct tcgtggttgc
     4021 cgttattggt gcgattgtcg tgctatttat ctacaggaag attaaaagtt aacgcttaaa
     4081 ttgcacaaag gctgcacaca ggcagccttt gctatttttt agaggtgacg ttaccaccat
     4141 cccagctcag tgccgagtac gacaataatc gccacaccca tcataataat cgttgttttt
     4201 ttcatcttta tgctcccggg gcagcatagc cgccaataaa aaaccatgct aaaaatacga
     4261 ccgctgtaat gaagattaca accggaaaaa taataccgat tcgcatgtta tcaccaatca
     4321 aaaggatgtg aatacgcctc aggaatgtag cgctggatgc gcgacaaaag gtgttttctg
     4381 tttgtgtttt ttctcctgat acatcgcaat atcggcagca tgcagggcac tgtctgcatc
     4441 ggttgtttca ggatcgactt ctactatgcc aagactggca ccgggataat acaaatcaac
     4501 atcacctaag tgatattctc cacgtatttg ctgctgaatg cgttctcgca gcgacgaaat
     4561 atccgcattc tcgttgttta gtgaaacaac taaaaactca tcaccgccga gacgaccaat
     4621 aacttctcca ttttgctgga gcgtattaag gcgctcgcca acctgaatga gaaacagatc
     4681 gccactatta tgaccaaaac gatcattgat taatttgaag ttatccagat cgataaacgc
     4741 gatcattatc ttatggttaa gatgccgggc gagggaaaac agtgtcgtca gattttcaaa
     4801 aatcgcccgc cgattcggta gcccggttaa cgagtcggta tacgattgcg caatcagcgc
     4861 agcattggct tcgcgcagct gttcaaccag tgcctctttt tgaatatatt gtgcaatcag
     4921 tccggcgaat aactgtaaaa cctgttccgc gcgttcactc cactggcgct tctcactgct
     4981 ggcggcgcaa agcgtgccat agaatgatcc atccggtaag tgaattggcg tgctcagaaa
     5041 tgtggtgatg ccaagattgc gtgccgcaat acagtcaccc caacggtcgg ggacttcatc
     5101 gctgaaaaag cagttttcat caatggcgcg tttgcataac gagtaatccc acgagacggt
     5161 aaaattctcc gggatgtgca ttttctggct gttacgggca aacataatat gctgcaggcg
     5221 cgcttcgaca tccactttgg tcaggtaggt tgattccata tcagtgacca tttccagcat
     5281 ctccagaagt tgtcggacca gactttccag tgactgttct ttggcaaggg attgcgagac
     5341 gcgggcgata atctgatctg acacggaaca actccaggat acggcgggta aatgctgatt
     5401 atttaatctt tgcaggcatg ataaaacatc atcaacaaag atgaaacaag acgtaatatg
     5461 ttttgagtga atgtaataaa aatagtgctg caaaacaaaa cgataccgcg atactctaat
     5521 cttggataaa tatgtgagat caataaatga aaattatcag ttttgttctg ccttgcttgc
     5581 tggtcctggc tggttgttca accccttctc agccagaagc acctaaaccg ccgcagattg
     5641 gtatggcaaa tccggcgtcg gtctattgcc agcagaaggg cgggacgctc attccagtgc
     5701 agacagcgca aggggtcagc aacaattgca aattaccggg cggtgaaacg attgatgaat
     5761 gggcattgtg gcggcgtgac catccggctg gtgaaaaata accgccggat gctgtactaa
     5821 aatcacaagc tacgtagcca gtcggccagc accagcgcat ggttctgcgt ggtgtttttt
     5881 gctgcgtaga gcagggtcag cggctgtttt ttggcgatgt ccgccagccg ctttccttct
     5941 tgctcgtgtt gtgccagttc tgcaagatat tgctcgcgaa aggttgcaaa atcgacgact
     6001 tcgccgtgaa aggctttgcg cagttccgtt gacggcgtga tttctttatc ccactcatca
     6061 agggctaaat cggttttttt gataccgcgc ggccagaggc ggtcgaccag tatgcgataa
     6121 ccatcgctct gttctgccgg atcataaacg cgtttgcact gaatattcat cgtgtttgtt
     6181 ctccctttca cgcatgattt gtcacgtcac tatagcaccg gatattagcg aatttctttg
     6241 tctcttccgg cgcaaagtcc gaaaatcgcc atcagtagtg aaagtattgc aacacccatt
     6301 agtggtacgg accagtcacc gttagcatcg tgtattttac ccatcagcgg cggcccacag
     6361 gctgccagca aatacccgac ggattgtgcc atccccgaga gtgccgccgc ctgatgcgca
     6421 gaacttgccc gaagaccaat gaacgtcaac cccagtatca ttgttgcgcc ggaaccaaaa
     6481 ccgaaaagca gagtccaggt gatcgcgtgt gctggcataa agcagagccc aaccgcgccc
     6541 actgcgcaca tcaaggcaac gaacgctgca ataccacgct gatctttcac atgatgtaag
     6601 aaaagtggga tcagcaaacc gggagctgct gtggctagtt gcagcaaacc atgcagtgaa
     6661 cccgcctgtg cttcgctata gccgtgactg atgaggatcg ccggaagcca gccaataatc
     6721 acgtaataga ccagtgagtt gatcccaaga aacaatgtga cctgccaggc aagcggcgaa
     6781 cgccagatac cccgagtatg taaggcgcgc gaggtactca aatttgcatg ttgttggctt
     6841 cgccactgtg gcagccataa aaaaagagcc agcagaggaa aacacatcag catgagcaac
     6901 gcgccttgcc agccaaaacc gttaaaagcc agcggcacta ccatagccga tccaagtgcc
     6961 gccgcagctc ccattgtcag ggaatatgcg ccggtaagtc tggcgacgga atgagggaaa
     7021 tcgcgtttaa ttaatcctgg cagtaagaca ttacctaatg caatcccacc accaatgacc
     7081 gctgtaccgc caaataataa gtaaggcgaa gggagagagc gaattgcgat accagcacag
     7141 atcaaaagta acgcggcaaa caggctacgt tccataccaa aacgtcgcgc taccggggcg
     7201 gccaaaggtg agattagcgc aaaggccaat aatggcaggg tggtcaataa gccggtttgc
     7261 gctgtcgtca gcgagtaagc ggaacgaatc gtatccagta acggtgctgc gccggtaaag
     7321 gtgacgcgta atgttgtggc aatcatcaga atgccagcga taaggacaat cctgtttttg
     7381 ccgcttaatg aagttgaaca ggtcatatgc ttctcattgt tacaaacgag gctgtacctt
     7441 accgttttta ttgttatgtt gaatcaagct aaaatgacaa attatcgcta aattcggaca
     7501 aaatgatgca caggttgaat ctcaatggct atgaacccga ccgacaccat gaagccgcgg
     7561 tcgcattttg tattcacgcc ggaacggatg agttaaccag tcctgtacat cagcaccgca
     7621 aaggacagct gatactggcg ctacatggtg caattacctg tacggtggaa aatgctttgt
     7681 ggatggtgcc gccgcaatat gcggtttgga taccgggcgg ggtggaacat agcaatcaag
     7741 tgacagctaa cgctgaactc tgttttctgt ttatcgaacc cagcgcagtg acaatgccga
     7801 caacatgttg cacattgaaa atatcgccct tatgccggga attgatttta accctggcaa
     7861 acaggacgac gactcagcgt gctgagccga tgacccacag attaattcag gtcctgtttg
     7921 atgaactccc tcagcagcca caacaacagc tgcacctgcc cgtttcttct catcctaaaa
     7981 tccgcacgat ggtggagatg atggcgaaag ggcccgtcga gtgggggtca ttagggcaat
     8041 gggctggctt ttttgcgatg agtgaacgca atcttgcgcg actgatagta aaagagacgg
     8101 ggttgagttt tcgccagtgg cgtcagcagt tacagctgat tatggcactt cagggattag
     8161 tgaaaggcga tactgtacag aaagttgctc atacgctggg gtatgattca acaacggcct
     8221 ttattactat gtttaagaaa ggcctggggc aaacgccagg ccgatatatt gcgggattaa
     8281 ctactgtttc cccacaatca gcgaaaccag acccgcggca ataagcgggc cgaccggtac
     8341 gccgcgaaac agcgccacgc ctaaaacagt gcctaccagc agcccggcga ccagttgcgg
     8401 ctggctgccc attaaggtca cgccgcgccc acccagccag gagacaatca cgcctactgc
     8461 aattgccacc agcgatttcc agttcagaaa cgagtgaatc aaggttgagg gcggtagcgt
     8521 cccgctggcg atgggggcca taacgccaat cgtcagaatg attatcccga tacttagccc
     8581 ctgtttttca atccacggga aaaaggtgct taacggtgtg acgcggacaa tgatcaacac
     8641 cagaattgaa acggcgacag tggtgttatg actgataaag cccagcgcag ctaacccgag
     8701 caggatcagc aaagtgacat caaacatcgt ggtttccttg ccaaaaaata atagcctgct
     8761 tactttacgc ttgctgcagg aaggaagtag aaaatcttta cgttttttgc ataaaaaatc
     8821 aggcgaactg tcatcaaact gccatttgtg cacgctaaca ataacgtgaa aacagctatc
     8881 tcccgggggc atgctatgaa cgatcaaatg ttcgtcgaga cactgattat cacgtcatcg
     8941 ttttttgcca ttgctgttgt gctggttttg tccgttctgc ttattgaacg gacaggctag
     9001 ttacgcagtg cggcggaaat tgagcagttc tggttgcgca atgcgcaaat aatccgtggt
     9061 tttcaaaata acggatttat ccagcatccc tgcattgaag gcgatttcat caaatcgttc
     9121 gaagagtaga gggtcggcaa ccagtttgag ttttggatgg aagctgaaag gggggatcgc
     9181 gccgaagaca cagcctgtga gttcatcgac ttccgccggg ctggccaacg aagcgcgtaa
     9241 tccgcctata tggctggcga gttggctcag atcggcctgt tgatcggcag cgaggatcgc
     9301 cagaacatgt tgatttacgc cattgccttt gactttacat accagtgcct tagctccctg
     9361 gcctaaagcg gtgccacgaa tttctgacac cgcttcacat ttacctaccg cttcatgggt
     9421 aacgacgcgg aagttagcac cttcttgtga taataaagcg attaaccgct gatgcgtcac
     9481 gcttccttta gccatttcag tcatttcacg ctcctgagat tacaagcaaa caaccacaga
     9541 aggttatcat gaacggctgt ttttgttctg cttattgaca tataaacgct catcggaagc
     9601 tttatacgca tcatgtaacg tatcgttttc tttcatcgcg taaataccgg aagagaagcc
     9661 gatctctttc tgcggcgcga tatgttgcag acgtttttcg atacgttcag gcagttgtgc
     9721 tgcaatttgc ggcgtcgaat cgacaagaat gatgcagaat tcatcgccac cgagtcggat
     9781 ggcataatca cttttacgaa tcgattgttt aatcgcctga gctaacaacg taatcgctaa
     9841 atccccttcc tgatgaccga gggtgtcatt tattagcttt aacttgtcca tgtcaattgc
     9901 aataaacatc accgaagaac cggattgcac cagtttctgc aaccgctgct ccagttcagg
     9961 ggttaaaatt ttgcgattat acagtccagt catcgcatca ctaatatttt ctcgcgacac
    10021 attttggtat aagcggaagt gcatccgcac catattcagc aaaattaacg ccgtcaaaat
    10081 ccagaacaga acgcttttcc acgaagacgt gataaagtaa agaatatcaa tggataacga
    10141 gacacgaatg ccgcccggta agtcatggac gtaactgaca tactggaaca gattatcttc
    10201 gctctggttg atgataatgt cgcgccccga atcggtatcg gttagcgtga cattgagaaa
    10261 acgccagagg agagggcggt catgagtgta aaagatattc cgcaaattgt ttttgttaat
    10321 atccagcagc acaatccctt ttagctgccc tgcaacatac accggcgtca gcaaactcat
    10381 cacgttctgt ttagtacgct gatcctggta aatgctcgac aatacggtac gcccggtaaa
    10441 taccttttca atatctttgc gatcgatacc aatacttcct gactgcagga aggaccagtt
    10501 attcatcgca aagtattcaa cattaaccgg ctcataaaaa tagacatagc ggtcattgag
    10561 atcgagataa tagtgaaatt tctcttcggc aatcccaaga cctgaaccgt aatcatcctg
    10621 gtggcggttg ccttccactg cctgatcaaa agcgggtaat agtgctgcag cttccacaat
    10681 tgtgtcgcag tcagtggatg cggtttgtag tgtgccgcgt aagcccgggt atttatgggc
    10741 agtcaggttt aagccatggg tattattggc agactcaaaa gcctcgcaga tggcgcgccg
    10801 ctgttctggc gacacttccg actgctcagc agcgagagcg cgcatcacat gcgcggcaat
    10861 actctgattc tgatacttat cataaagaaa ggaggagtct gctttctgca cgatatagcg
    10921 cagataggca ctcattgctc tgtcgctggc gacaagttca aaaataagga aggaagatgt
    10981 cagcacaatg acacttgcgg cgataaaatg ccggagcatt ctatggtgca atttcatgat
    11041 aaatggcact ccttcggtta gtgaaacgag aaaaccagat aaagttcgat tgcggcctgg
    11101 cacaggggcg ctaattatac tcatctctgc gccgctgggg aggggaaatt taggggtaat
    11161 agtaataaat tgtatgcaaa agccacgcaa aaagcatggc tctgaatatt tttgcactta
    11221 aatatgatga ataatcactt tgttgccccc ctcgcttttc gcttcgcgta aggctttatc
    11281 tgcttccgtc atgacaagcg agatattacg cgtgtcaccc gttgaaaaaa ccgtgccaat
    11341 actgatggtc atttgttcag gaacatcata tctgttacta aagccggtca ggagttcgac
    11401 atttttacgc atacgttccg caatgatttt tgcctgggca ctattgagtt cggtaaatag
    11461 caagccaaac acctcaccct ccagtcgcgc taaaatatca tcagggcgga tgctttgctg
    11521 gataatatcg acaattgaaa ccagcacttt atcacccaca cgatgtcccc attgggcatt
    11581 gatgcgtttg aactgattaa tatcaaccag catgacacag aagtccttaa caacaggtcg
    11641 cgacaataat gactctacgc tgttgaaaaa gtagcgccga ttataaatat tggtcagcac
    11701 atcatgaact gccagtttgc tggagagttg cagttcctga aaaatgttat aaatcagaaa
    11761 agacacaaca aacagtttac tggaaacttc aatcccgcga ctgatatacc agatggtgta
    11821 attatactca tccagagtca gtagaagtaa gttgcagcat agcgatgcca gacataataa
    11881 tgctattaac ggccatagtt cgctggctaa tcgtgtacgc atgataataa agaacagtaa
    11941 aaatgcccac agacaaacca atatttttgt atagttgatt ccccaggtcg cagtatggtt
    12001 gtccggacag tagtcgacga catacaaaga atagtcagcg ttataactgc tcagattgtg
    12061 tgccagaagg ggaaaaacta aaaaaggaat cagcgccagc aacaggattc ccgttttttt
    12121 cttattgttg tcaaggatgt tgtcttttcc ataacaaaag agtgccagcg aggttaaaca
    12181 aataaaactg agttggcgga aaatatagaa aatagaaaca tcattcgact ttgtctggat
    12241 cagtgtactg ccgttaattg gtttatggat aattacaatg gtttcaacta aatagataag
    12301 gccacttaaa aatgcaaggc ttaaaatgag tgttggtaaa atttgtttgt tagtgaaata
    12361 tttcattaac atgaaaagaa aaatgacggc gttaaggaaa aacaaagcca caacaaccat
    12421 caggtaattc atctgtggga tgagttttac atttgtgttt tcgttatata atgtggtgat
    12481 gcaaaataaa attaacgtca gcaataaact gaaaataaaa aagctttgag cttttaattt
    12541 ttgtgtaccc atagaaacca taaaaaggag agcaatgtga gtgctggatt ttattgatgg
    12601 cagagatata tagttttgac caggatcaat tttcgttaac gataaaaaat atttaaagaa
    12661 taaccccatg gaaatacgtg ttgactgaat catttttccg aatgattgtt ttctggaac
//
LOCUS       DAFNBJ010000136        12717 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000136 DAFNBJ010000000
VERSION     DAFNBJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.136"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            39..1553
                     /gene="caiT"
                     /locus_tag="L2X49_003720"
     CDS             39..1553
                     /gene="caiT"
                     /locus_tag="L2X49_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBN4798741.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1584..2726
                     /gene="caiA"
                     /locus_tag="L2X49_003721"
     CDS             1584..2726
                     /gene="caiA"
                     /locus_tag="L2X49_003721"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBN4798742.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2855..4072
                     /gene="caiB"
                     /locus_tag="L2X49_003722"
     CDS             2855..4072
                     /gene="caiB"
                     /locus_tag="L2X49_003722"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBN4798743.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLSTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4146..5699
                     /gene="caiC"
                     /locus_tag="L2X49_003723"
     CDS             4146..5699
                     /gene="caiC"
                     /locus_tag="L2X49_003723"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBN4798744.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5808..6593
                     /gene="caiD"
                     /locus_tag="L2X49_003724"
     CDS             5808..6593
                     /gene="caiD"
                     /locus_tag="L2X49_003724"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBN4798745.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGTEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAVEGPLAFAEKRDPVWKGR"
     gene            6599..7189
                     /gene="caiE"
                     /locus_tag="L2X49_003725"
     CDS             6599..7189
                     /gene="caiE"
                     /locus_tag="L2X49_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBN4798746.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7275..7670)
                     /gene="caiF"
                     /locus_tag="L2X49_003726"
     CDS             complement(7275..7670)
                     /gene="caiF"
                     /locus_tag="L2X49_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBN4798747.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7931..11152)
                     /gene="carB"
                     /locus_tag="L2X49_003727"
     CDS             complement(7931..11152)
                     /gene="carB"
                     /locus_tag="L2X49_003727"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBN4798748.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11170..12318)
                     /gene="carA"
                     /locus_tag="L2X49_003728"
     CDS             complement(11170..12318)
                     /gene="carA"
                     /locus_tag="L2X49_003728"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBN4798749.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2994 a         3306 c         3362 g         3055 t
ORIGIN      
        1 cgcaaaataa atattcgttt tcacagtgga attaactcat gaagaatgaa aagagaaaaa
       61 cgggaataga accgaaggtt ttctttccgc cgttaataat cgtcggcata ctttgttggc
      121 ttacagtcag agatctggat gcagcgaatg tcgttattaa tgctgtattc agttacgtca
      181 ccaatgtatg gggatgggca tttgaatggt atatggtggt gatgcttttc ggttggttct
      241 ggctggtgtt tggcccgtat gccaaaaagc gtttaggtaa cgaaccacct gaatttagta
      301 ccgccagttg gatctttatg atgttcgcct cctgtacgtc tgctgccgta ctgttctggg
      361 gatcgattga gatctactac tacatctcca ccccgccgtt tggcttagaa ccgaactcga
      421 caggagcgaa agagttgggg ctggcttaca gcttgttcca ctggggacct ctgccgtggg
      481 ccacttacag cttcctttca gtcgccttcg cttacttctt ctttgtccgc aaaatggaag
      541 tgattcgccc cagctcgaca ctggtgccgc tggtaggtga aaaacacgcc aaagggttgt
      601 tcggcactat cgtcgacaac ttctatctcg tcgccttgat cttcgcgatg ggtaccagtc
      661 tgggccttgc cacgccgctg gtgaccgagt gtatgcaatg gttgtttggc attccgcata
      721 ccctgcaact ggacgctatc atcattacct gctggattat cctcaacgcc atttgcgtcg
      781 cttgcggtct gcaaaaaggg gtacgtatcg ccagtgacgt gcgtagttac ctgagcttcc
      841 tgatgctggg ttgggtgttc attgtcagcg gtgccagctt catcatgaac tacttcaccg
      901 attcggtggg gatgttgctg atgtatctgc cgcgcatgtt gttctatacc gatcccatcg
      961 ctaaaggcgg cttcccgcag ggctggaccg tgttctactg ggcatggtgg gtgatttatg
     1021 ctatccagat gagtatcttc ctcgcccgca tctcccgtgg tcgtactgtg cgtgaactgt
     1081 gcttcggcat ggtgctgggg ctgacagcgt caacctggat cctgtggact gtactcggta
     1141 gtaacactct gctgttgata gataaaaaca tcatcaacat tccaaatctg atcgaacagt
     1201 acggtgtggc gcgcgccatc attgaaacct gggccgctct gccactcagc accgccacca
     1261 tgtggggctt cttcatcctc tgctttattg ccaccgttac gctggttaac gcctgctctt
     1321 ataccctggc gatgtccact tgccgcgaag tacgcgatgg tgaagaacca cctctgctgg
     1381 tgcgtatcgg ttggtcaatt ctggttggca ttatcggtat tgttctgctg gcgctcggcg
     1441 gcctgaaacc gattcaaacc gccattatcg ccggaggatg cccgctgttc ttcgtcaaca
     1501 ttatggtgac gctctccttt attaaagacg cgaaacagaa ctggaaagat taattaaccc
     1561 ccaaaatatc aagaggttga aagatggatt ttaatttaaa tgatgagcag gaactgtttg
     1621 tcgccggtat ccgcgaactg atggccagcg aaaactggga ggcctatttt gccgagtgcg
     1681 accgtgacag cgtctacccg gaacgttttg tcaaagcact ggcggatatg ggtatcgaca
     1741 gtctgctgat ccctgaagag cacggtggtc tggacgcggg gtttgttact ctcgccgccg
     1801 tgtggatgga gctgggacgt ctgggggcac caacctatgt gctgtaccag ttgccgggcg
     1861 ggttcaacac cttcctgcgc gaaggcacac aagagcagat cgacaagatt atggctttcc
     1921 gcggcaccgg taagcagatg tggaactcag cgattaccga accgggcgcg ggctccgacg
     1981 tgggtagcct gaaaacgact tatacccgta gaaatggtaa gatttatctt aatggtagta
     2041 agtgttttat taccagcagc gcctacaccc cgtacatcgt ggtgatggcg cgcgacgggg
     2101 cttctccgga caaacctgtc tacaccgaat ggtttgttga tatgagcaaa ccgggcatca
     2161 aagtgaccaa acttgaaaag ctcggtctgc gtatggatag ctgctgtgaa atcacctttg
     2221 acgacgtgga actggacgag aaagacatgt tcggtcggga aggtaacggc tttaaccgcg
     2281 tcaaagaaga gttcgatcat gaacgtttcc tggtagctct caccaactac ggtacggcga
     2341 tgtgcgcctt tgaagatgcg gcgcgctacg ccaaccagcg cgtgcagttt ggcgaggcta
     2401 ttggccgttt ccagttgatt caggaaaaat tcgcccacat ggcgatcaaa ttaaactcca
     2461 tgaaaaacat gctgtatgaa gcagcgtgga aagcagacaa cggcaccatc acctctggcg
     2521 atgcagcgat gtgcaaatac ttctgcgcca atgcggcatt tgaagtggtg gatagcgcaa
     2581 tgcaggtgct gggcggtgtc gggattgcgg gcaaccaccg catcagccgc ttctggcgtg
     2641 acctgcgtgt agaccgcgtc tccgggggat ctgacgaaat gcagatcctg acgctgggtc
     2701 gcgcggtgct gaagcaatac cgctaagtcc gtgcgctgcc tgatgcgacg ctaatgcgtc
     2761 ttatcaggcc tacgggagca cgaatgtagg ccggataaag cgtttacgcc gcatccggca
     2821 gtcatgcgct acataaattt ttcaggagct aataatggat catctaccca tgccgaaatt
     2881 cgggccgttg gccggattgc gcgttgtctt ctccggtatc gaaatcgccg ggccgttcgc
     2941 cgggcaaatg ttcgcagaat ggggtgcgga agttatctgg atcgagaacg tcgcctgggc
     3001 cgacaccatt cgcgttcaac cgaactaccc gcagctctcc cgccgcaatt tgcacgcgct
     3061 gtcgttaaat attttcaaag atgaaggccg cgaagcgttt ctgaaattaa tggaaaccac
     3121 cgatatcttc atcgaagcca gcaaaggtcc ggcctttgcc cgtcgtggca ttaccgatga
     3181 agtactgtgg cagcacaacc cgaaactggt tatcgctcac ctgtccggtt ttggtcagta
     3241 cggcaccgag gagtacacca atcttccggc ctataacacc atcgcccagg ccttcagtgg
     3301 ttacctgatt cagaacggtg atgttgacca gccaatgcct gccttcccgt ataccgccga
     3361 ttacttttct ggcctgaccg ccaccacggc ggcgctggca gcactgcata aagcgcgtga
     3421 aaccggtaaa ggcgaaagta tcgacatcgc catgtatgaa gtgatgctgc gtatgggcca
     3481 gtacttcatg atggattact tcaacggcgg cgaaatgtgc ccgcgcatga gcaaaggtaa
     3541 agatccctac tacgccggtt gcggtctgta taaatgtgcc gacggctaca tcgtgatgga
     3601 actggtgggc attacccaaa ttgaagagtg ctttaaagat attggcctcg cacatctact
     3661 cagtacgccg gaaatcccgg aaggcactca gcttatccac cgtatcgaat gcccttacgg
     3721 cccactggta gaagaaaaac tcgatgcctg gctggcggca cataccatcg cagaagttaa
     3781 agaacgcttt gccgaactga atatcgcctg tgccaaagta ctgaccgtac cggaactgga
     3841 aagcaatcca cagtatgttg cccgtgaatc aatcactcag tggcaaacga tggatggtcg
     3901 cacctgcaaa gggccgaaca tcatgccgaa attcaaaaat aaccccggac aaatctggcg
     3961 cggaatgccc tcacatggca tggacacggc tgccattttg aaaaatatcg gctacagcga
     4021 aaacgacatt caggagttgg tcagcaaagg tctggccaaa gttgaggact aaacgcgtgg
     4081 cgtcagtcgg cttgcctgac tagcgcccgc ccaataacaa acgaacaaaa atggatagag
     4141 gtgcaatgga tatcattggc ggacaacatc tacgtcaaat gtgggacgat ctggcggacg
     4201 tttacggtca taaaacggcg ctgatttgtg aatccagcgg cggagtcgtt aaccggtata
     4261 gttatcttga gttaaatcag gagattaacc gcacggcaaa cctgttttat acgctgggga
     4321 ttcgcaaagg cgacaaggtt gcactacatc tcgacaactg cccggaattt atcttttgct
     4381 ggttcgggct ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc ctgttacgcg
     4441 aggaaagcgc gtggatcctg caaaatagcc aggcgtgcct gctggtgacc agtgcgcaat
     4501 tctatcctat gtatcaacag attcagcagg aagatgccac tcaattgcgg cacatttgcc
     4561 tgacagatgt ggcacttccc gctgatgatg gcgtgagttc gtttactcaa ctgaaaaatc
     4621 aacaacctgc caccttgtgc tatgcaccgc cgctattgac tgacgatacg gcggaaattc
     4681 tcttcacctc cggcaccacc tcccgaccga aaggtgtggt gattacccat tacaacctgc
     4741 gcttcgctgg atattactcc gcctggcagt gtgcactgcg tgacgatgac gtctacctga
     4801 cggtaatgcc tgcgtttcat atcgattgcc agtgtactgc ggcgatggcg gcgttttctg
     4861 ccggggccac ctttgtgctg gtcgagaaat acagcgcccg cgccttctgg ggacaggtgc
     4921 agaagtaccg cgccaccatt accgaatgta ttccgatgat gatccgtacg ttgatggtgc
     4981 agccgccttc agcgaacgat cggcaacacc gcctgcggga agtgatgttt tatctcaact
     5041 tgtcggagca ggaaaaagat gcgttttgtg aacgcttcgg cgttcgcttg ctgacgtctt
     5101 atgggatgac ggaaaccatt gtgggcatta tcggcgatcg ccctggcgat aaacgacgct
     5161 ggccgtcgat tggtcgggcg gggttttgct acgaagcgga gatccgcgac gatcacaatc
     5221 gcccgctccc ggcaggtgag atcggtgaaa tctgtattaa aggcgtacca gggaaaacca
     5281 tcttcaaaga gtattttctc aacccgaaag ccactgcgaa agtgctggaa gccgacggct
     5341 ggctacatac cggcgatacc ggatactgcg acgaagaggg ctttttttat ttcgtcgatc
     5401 gccgctgcaa tatgatcaaa cgcggtggcg agaatgtctc ctgcgtggag ctggaaaata
     5461 tcatcgccac ccatccaaaa attcaggata tcgtggttgt gggtattaaa gattcgattc
     5521 gcgatgaagc catcaaagca tttgtggtgc tgaatgaagg tgaaacattg agcgaagagg
     5581 aatttttccg cttctgcgaa caaaatatgg cgaaatttaa agtgccctct tatctggaga
     5641 tcagaaaaga tctgccacgt aattgctcgg ggaaaataat tagaaagaat ctgaaataaa
     5701 tgaaacagca gggaactacc ctgccagcta ataatcacgc tataaaacaa tatgcatttt
     5761 ttgcagggat gttgtcgtcc ctgaaaaagc aaaaatggag aaaaggaatg agtgaatcat
     5821 tacatctgac ccgcaatgga tcaattctgg aaattaccct tgatcgtcca aaagcgaatg
     5881 ctattgatgc aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc
     5941 cgcaattacg tgtcgccatt attaccggtg ccggagagaa gttcttttcc gcgggctggg
     6001 atttaaaagc ggcagcagaa ggcgaagcac cggatgctga ctttggcccg ggtggatttg
     6061 cgggattaac cgaaattttc aatctcgaca aaccggttat cgcagctgtg aacggctatg
     6121 cctttggcgg cggtttcgaa ctggcgctgg cggcagattt tattgtttgt gccgataacg
     6181 ccagcttcgc cctgccggaa gccaagctgg gcatcgttcc tgacagcggc ggcgtgctgc
     6241 gtctaccgaa gatcctgccg cctgccatcg tcaatgaaat ggtgatgacc ggcagacgaa
     6301 tgggcacaga agaggcgctg cgttggggga tagtcaaccg cgtggttagc caggcggaac
     6361 tgatggataa cgcccgcgaa ctggcgcagc agctggttaa cagcgccccg ctggcgattg
     6421 cggcgctgaa agagatctac cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct
     6481 atattcgcag cggcgtgttg aaacactatc cgtcggttct gcattcggaa gatgccgttg
     6541 aagggccgct ggcgtttgcc gagaagcgcg atccggtgtg gaaaggacgt taacaaccgt
     6601 gagctattac gcctttgagg ggttaattcc ggtggttcac ccgacggcgt ttgtccatcc
     6661 cagtgccgtc ttgattggcg atgtgattgt gggagccggt gtctacatcg gcccactcgc
     6721 ctcactgcgt ggtgactacg ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg
     6781 ctgcattatg catggctact gcgacactga caccatcgtt ggggaaaacg gccatatcgg
     6841 gcacggagcg atcctgcatg gttgtgtgat tggtcgcgat gcattggtcg ggatgaacag
     6901 cgtgattatg gatggcgcgg tcattggcga agagagcatt gttgccgcca tgagctttgt
     6961 caaagcaggc tttcacggcg agaaacgcca gttgttgatg ggtacgccag cccgcgctgt
     7021 acgcagtgtt agtgacgacg agttacactg gaaacggttg aataccaaag agtatcagga
     7081 tcttgtcggg cgctgtcatg catcgttaca tgaaacacag ccactgaggc aaatggagga
     7141 aaatcgcccc cgtttgcagg ggacgacgga tgtgacgcct aaacggtgat cttgccggat
     7201 gcgacctggt tgctggataa gatgctacaa catcgcatct ggcaagcagg acgcccggta
     7261 tcaacgagat aaaattaacg acgcatactc tttgacaaca tcatctgcca cttctgctcg
     7321 cggttaagtt ccgtgagcgg aacggcagga atacgcggcg tctttcttac gccgactaat
     7381 ttatcccggc tgttattgcg caggcgcgcg taaatttgct catcgatatc gaccactttt
     7441 accagtcgct ggcactggca tccccgtcct tccagcttat tagggatcat cttaacttcg
     7501 cagcttattt ctgcgacttc cgacagaata taagtcaggg tattaaccgc cttgctgtgt
     7561 tcaatatcga aatgaataga gatctctctt gctatcaccc accgattttc agccatcatc
     7621 cattcggcga ttaacaagta gagtggtttt tcaacatatc cttcacacat acgaactcca
     7681 gtttcacaaa ttaaaagtct ggctatgact cgttttgaag caattaaaca aggccatatt
     7741 aaaattgtgg atatatcgat agtatgcaac ttcaccgtcg ataagtgtga gctaaatcct
     7801 gcttatgtca tcaaaaatat cgattcccca aaagatttac ttaatgggca aaaaaaacgg
     7861 tcctcatcag agaaccgtat tacttattcg atcaaattag cggatgaaaa atatccgcca
     7921 tgacacgcta ttatttgatc tgcgcgtgca tttcctgcac cgaaattact ttttcggtcg
     7981 cgtcggcatt cagcgccatc gcggtagcga aaccaccgtt cagggtggtg tcataatgca
     8041 ctttatattg cagcgcactg cgacggatca cgcgggagtc ttcaatcgcg cgacggcctg
     8101 aagtggtgtt gatgatgtag gtatattcgc cattcttgat gcggtcctga atgtgcggac
     8161 ggccttcatg caccttgttc accagacgcg gattaatgcc cgcttcgccc agcacaatcg
     8221 ccgtgccgtg ggtcgcatcc agctcgaagc cctgtttcag cagttttgcc gccaggtcca
     8281 ccacgcgttc tttatcgcct tcgcgcacgg aaagcaacgc acgaccgtgt ttcttcatgg
     8341 tggagttgct gcccagctgc gctttggcaa acgcttcagc gaaggtgcgg cccacgccca
     8401 tcacttcccc ggtggagcgc atttctggcc ctaacagcgg gtcaacaccc gggaatttat
     8461 tgaacggcag caccacttct ttcaccgagt agtacggcgg gataacttct ttggttacgc
     8521 cctgctcagc cagcgatttg ccagccatca cacgcgccgc cacttttgcc agcggtacgc
     8581 cggtggcttt ggagacgaac ggaacggtac gcgccgcacg cgggttaact tcaatcaggt
     8641 agacttcgtt gtttttcacc gcaaactgca cgttcatcag accgcgcacc tgcaattcga
     8701 aggccagttt ctgcacctgc tggcgcatca catcctgaat ttcctgactt aaggtgtagg
     8761 ctggcagaga acatgcggag tcaccggagt gcacgcccgc ctgctcgatg tgctccatga
     8821 tgccgccaat cagcaccatt tcgccgtcgc agatggcatc cacgtcaact tctaccgcgt
     8881 catcaaggaa gtgatccagc aacactggcg catcgttaga cacgctgacc gccgtctgga
     8941 agtagcgacg caggtcagct tcgtcataga cgatttccat cgcccgaccg ccgagaacgt
     9001 aagacggacg taccaccagc gggtagccaa tctctttcgc cttctctacc gccatttcaa
     9061 tagcggtaac ggtggcgttc gccggttgtt tcagtttcag acgctcaacc gcatgctgga
     9121 agcgttcacg gtcttctgca cggtcgatag catccgggct ggtgccgata accggtacgc
     9181 cagcagcttc cagcgcgcgc gccagtttca gcggggtctg accgccgtac tgaacgataa
     9241 cgcctttcgg cttctcaata cgcacgattt ctagcacatc ttccagagtt accggctcga
     9301 agtagaggcg atcggaagtg tcgtagtcgg tggagacggt ttccgggtta cagttaacca
     9361 taatggtttc gtaaccgtct tcgcgcagcg ccagcgaggc gtgtacacag cagtagtcga
     9421 attcgatacc ctgaccgata cggttcgggc cgccgccgag aaccatgatt ttttcacggt
     9481 cggtagacgg attcgcttcg cactcttctt cataagtgga gtacatgtaa gcggtgtcgg
     9541 tggcgaactc tgccgcacag gtatccacgc gcttataaac cgggtgcagg tcatactggt
     9601 cgcgcagctt gcggatttcc gcttcgcgca cgcccgccag ttttgccaga cgcgcatcgg
     9661 caaagccttt gcgtttcagc tggcgcagga attcagcgtt caggccagtg atgcccactt
     9721 ccgctacttt ctcttccaga cgcaccagtt cttcaatctg taccaggaac cagcggtcaa
     9781 tattggtcag gttgaacacg ccgtccacag acaggcccgc acggaaggca tcggcgatgt
     9841 accagatacg ctctgcgcca gcatctttca gttcgcgacg gattttggtt aacgcttccg
     9901 ggtcatcgag gctcactttc gggtcgaatc cagtcgcacc gacttccagg ccgcgcagcg
     9961 ctttttgcag ggattcctgc tgcgtgcgac caatcgccat cacttcgcca accgatttca
    10021 tctgagtggt cagacggtcg ttagcaccag cgaatttttc gaagttgaag cgaggaattt
    10081 tagtaaccac atagtcgatg gacggctcga aggaggccgg agtacgtccg ccagtgatgt
    10141 cgttcatcag ttcgtcgagg gtgtaaccca ccgccagttt cgccgccact ttagcaatcg
    10201 ggaaaccggt cgctttcgac gccagcgccg aagaacggga cacgcgtggg ttcatttcga
    10261 taacaatcag gcgaccgttt ttcgggttca ccgcaaactg gacattggaa ccaccggttt
    10321 caacgccgat ttcacgcagc accgccatcg aggcgttacg catgatttga tattctttgt
    10381 cggtcagcgt ttgggctggc gcgacagtga tggagtcacc ggtatggatg cccatcgcat
    10441 caaagttttc gatagagcag acgatgatgc agttgtcgtt tttatcacgc accacttcca
    10501 tctcgtactc tttccagccg atcagcgact catcaatcag cagctctttg gttggggaga
    10561 gatccagacc gcgggcgcaa atttcttcaa actcttcgcg gttataagcg ataccgccgc
    10621 cgctaccgcc catggtaaag gatgggcgaa taatgcacgg gaagcccacg tcagcggcaa
    10681 ccgccagcgc ttcttccatc gtgtgtgcga taccggaacg cgcggtttcc agaccaattt
    10741 tcttcatcgc tacgtcgaaa cgacggcggt cttctgcttt atcaatcgca tcggcggtcg
    10801 caccaatcat agtcacgccg aactcttcca acacgccctg acgctccagc tccagcgcgc
    10861 agttcagcgc cgtctgaccg cccatcgttg gcagcaccgc gtccgggcgc tctttttcaa
    10921 taatcttgcg tactacttcc cagtgaatcg gctcgatgta ggtcgcatcg gccatttccg
    10981 ggtcggtcat gatggtcgcc gggttggagt tcaccagaat gacgcggtaa ccctcttcgc
    11041 gcagggcttt acatgcttgc gcgccagagt agtcaaactc acacgcctga ccgataacaa
    11101 tcgggcccgc acccagaatc aggatacttt ttatatctgt acgttttggc atggctcttt
    11161 tactcctgat tacttagcgg ttttacggta ctgctcaatt aactcgataa agtggtcgaa
    11221 caacggcgct gcgtcgtgtg gcccagggct ggcttccggg tgaccctgga agctgaacgc
    11281 cggtttatca gtgcgatgaa tgccctgtaa cgtaccgtcg aacagggatt tatgcgtgac
    11341 acgcaggttt gcaggtaatg ttgcttcatc caccgcaaaa ccgtggttct gggcggtgat
    11401 catcaccacg tttttctcca catctttaac cggatggttg ccgccgtggt gaccaaattt
    11461 cattttgaca gtcttcgcac cgctcgccag cgccagcagc tgatgaccga ggcagatgcc
    11521 gaataccgga atatcggttt cgaggaattt ctggatggcg gtaatggcgt aatcgcacgg
    11581 ggccgggtcg ccaggaccgt tggagaggaa gatgccgtct ggattcattt tcagcacatc
    11641 ttccgcagaa gtttgcgccg gaacgatggt caggcgacag cctctatcca ccagcatccg
    11701 caggatgttg cgcttggcac caaaatcata agccacgacg tggaacggca gctcgtcttc
    11761 ttttttcgct tctggcaggc cgccggtcaa cgtccagctc ccttgtgtcc agctataggc
    11821 ttctgcggtg gtcacttctt ttgccagatc catgccattc agacctggga acgcgcgggc
    11881 tttttctaac gccaacgccg catccgggtt atcgcctgcg ataatgcagc cattctgtgc
    11941 gcctttctcg cgcagtaaac gcgtcagctt acgggtatcg atatcggcaa tcgccacgat
    12001 gttatggcgc ttcaggtaag aagaaaggtc ttcggtatta cggaagttgc tggcaatcag
    12061 cggcagatcg cgaatcacca gaccttgtgc atgtacctga gaagattctt catcggcgtc
    12121 attagtgccg acattgccaa tatggggata agtaagagta acgatttgac gagaatagga
    12181 aggatcagtg aggatttctt gataaccggt cattgaagta ttgaaaacga cttccccaac
    12241 cgccgaacct gttgccccta tggcccgacc gtgaaactgg gttccgtctt ccagaaccaa
    12301 tagcgctgac ttaatcaaaa caccctccag agaatattca ctcactttat ttgcatatta
    12361 attcacaatg atgacgtaaa tcaatgcaaa tctgcttacc gacgaatttc tggcaaacgg
    12421 cggcattctg gagatatggg cgctaaaagt caacttaatg gcgggatttt ttatattatt
    12481 ttgtggctca tgcatgtttt acaggcatta agcgacaaaa agatcatata acctgttctg
    12541 gaaaacgctt gcgcagcaag aagattgcat gaaataaaag cgagcaggga aaagtggacc
    12601 aaatggtcaa aatttacatt aaaacaacaa aaaccgaaga caaaacatgt aatttttggc
    12661 acttatagac aatttaaaaa cataacacta aaaataaaag ggccaaaaat taaagcc
//
LOCUS       DAFNBJ010000137        12622 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000137 DAFNBJ010000000
VERSION     DAFNBJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.137"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(92..1375)
                     /gene="gltA"
                     /locus_tag="L2X49_003729"
     CDS             complement(92..1375)
                     /gene="gltA"
                     /locus_tag="L2X49_003729"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBN4798750.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1581..1688)
                     /locus_tag="L2X49_003730"
     CDS             complement(1581..1688)
                     /locus_tag="L2X49_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798751.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2069..2473
                     /gene="sdhC"
                     /locus_tag="L2X49_003731"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2069..2473
                     /gene="sdhC"
                     /locus_tag="L2X49_003731"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBN4798752.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2467..2814
                     /gene="sdhD"
                     /locus_tag="L2X49_003732"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2467..2814
                     /gene="sdhD"
                     /locus_tag="L2X49_003732"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBN4798753.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2814..4580
                     /gene="sdhA"
                     /locus_tag="L2X49_003733"
     CDS             2814..4580
                     /gene="sdhA"
                     /locus_tag="L2X49_003733"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBN4798754.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4596..5312
                     /gene="sdhB"
                     /locus_tag="L2X49_003734"
     CDS             4596..5312
                     /gene="sdhB"
                     /locus_tag="L2X49_003734"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBN4798755.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPTREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5613..8414
                     /gene="sucA"
                     /locus_tag="L2X49_003735"
     CDS             5613..8414
                     /gene="sucA"
                     /locus_tag="L2X49_003735"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBN4798756.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8429..9646
                     /gene="odhB"
                     /locus_tag="L2X49_003736"
     CDS             8429..9646
                     /gene="odhB"
                     /locus_tag="L2X49_003736"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBN4798757.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAIKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9740..10906
                     /gene="sucC"
                     /locus_tag="L2X49_003737"
     CDS             9740..10906
                     /gene="sucC"
                     /locus_tag="L2X49_003737"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBN4798758.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10906..11775
                     /gene="sucD"
                     /locus_tag="L2X49_003738"
     CDS             10906..11775
                     /gene="sucD"
                     /locus_tag="L2X49_003738"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBN4798759.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11879..12601)
                     /gene="mngR"
                     /locus_tag="L2X49_003739"
     CDS             complement(11879..12601)
                     /gene="mngR"
                     /locus_tag="L2X49_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBN4798760.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         3030 a         3217 c         3421 g         2954 t
ORIGIN      
        1 ttatttttac aattttttgt gtttaggcat ataaaaatta acccgccatt tgaacggcgg
       61 gttaaaatat ttacaactta gcaatcaacc attaacgctt gatatcgctt ttaaagtcgc
      121 gtttttcata tcctgtatac agctgacgcg gacgggcaat cttcataccg tcactgtgca
      181 tttcgctcca gtgggcgatc cagccaacgg tacgtgccat tgcgaaaatg acggtgaaca
      241 tggaagacgg aatacccatc gctttcagga tgataccaga gtagaaatcg acgttcgggt
      301 acagtttctt ctcgataaag tacgggtcgt tcagcgcgat gttttccagc tccatagcca
      361 cttccagcag gtcatctttg gtgcccagct ctttcagaac ttcatggcag gtttcacgca
      421 ttacggtggc gcgcgggtcg taatttttgt acacgcggtg accgaagccc atcaggcgga
      481 aagaatcatt tttatctttc gcacgacgaa caaattccgg aatgtgttta acggagctaa
      541 tttcttccag cattttcagc gccgcttcgt tagcaccacc gtgcgcaggt ccccacagtg
      601 aagcaatacc tgctgcgata caggcaaacg ggttcgcacc cgaagagcca gcggtacgca
      661 cggtggaggt agaggcgttc tgttcgtggt cagcgtgcag gatcagaata cggtccatag
      721 cacgttccag aatcggatta acttcatacg gttcgcacgg cgtggagaac atcatattca
      781 ggaagttacc ggcataggag agatcgttgc gcggataaac aaatggctga ccaatggaat
      841 acttgtaaca catcgcggcc atggtcggca ttttcgacag caggcggaac gcggcaattt
      901 cacgatgacg aggattgttt acatccagcg agtcgtgata gaacgccgcc agcgcgccgg
      961 taataccaca catgactgcc attggatgtg agtcgcgacg gaaagcgtgg aacagacggg
     1021 taatctgctc gtggatcatg gtatgacggg tcaccgtagt tttaaattcg tcatactgtt
     1081 cctgagtcgg tttttcacca ttcagcagga tgtaacaaac ttccaggtag ttagaatcgg
     1141 tcgccagctg atcgatcggg aaaccgcggt gcagcaaaat accttcatca ccatcaataa
     1201 aagtaatttt agattcgcag gatgcggttg aagtgaagcc tgggtcaaag gtgaacacac
     1261 cttttgaacc gagagtacgg atatcaataa catcttgacc cagcgtgcct ttcagcacat
     1321 ccagttcaac agctgtgtcc ccgttgaggg tgagttttgc ttttgtatca gccatttaag
     1381 gtctccttag cgccttatta cgtaagactg ccggaactta aatttgcctt cgcacatcaa
     1441 cctggcttta cccgtttgtt atttggctcg ccgctctgtg aaagagggga aaacctgggt
     1501 acagagctct gggcgcttgc aggtaaagga tccattgatg acgaataaat ggcgaatcaa
     1561 gtacttagca atccgaatta ttaaacttgt ctaccactaa taactgtccc gaatgaattg
     1621 gtcaatactt ccacactgtt acataagtta atcttaggtg aaataccgac ttcataactt
     1681 ttacgcatta tatgcttttc ctggtaatgt ttgtaacaac tttgttgaat gattgtcaaa
     1741 ttagatgatt aaaaattaaa taaatgttgt tatcgtgacc tggatcactg ttcaggataa
     1801 aacccgacaa actatatgta ggttaattgt aatgattttg tgaacagcct atactgccgc
     1861 caggtctccg gaacaccctg caatcccgag ccacccagcg ttgtaacgtg tcgttttcgc
     1921 atctggaagc agtgttttgc atgacgcgca gttatagaaa ggacgctgtc tgacccgcaa
     1981 gcagaccgga ggaaggaaat cccgacgtct ccaggtaaca gaaagttaac ctctgtgccc
     2041 gtagtcccca gggaataata agaacagcat gtgggcgtta ttcatgataa gaaatgtgaa
     2101 aaaacaaaga cctgttaatc tggacctaca gaccatccgg ttccccatca cggcgatagc
     2161 gtccattctc catcgcgttt ccggtgtgat cacctttgtt gcagtgggca tcctgctgtg
     2221 gcttctgggt accagcctct cttcccctga aggtttcgag caagcttccg cgattatggg
     2281 cagcttcttc gtcaaattta tcatgtgggg catccttacc gctctggcgt atcacgtcgt
     2341 cgtaggtatt cgccacatga tgatggattt tggctatctg gaagaaacat tcgaagcggg
     2401 taaacgctcc gccaaaatct cctttgttat tactgtcgtg ctttcacttc tcgcaggagt
     2461 cctcgtatgg taagcaacgc ctccgcatta ggacgcaatg gcgtacatga tttcatcctc
     2521 gttcgcgcta ccgctatcgt cctgacgctc tacatcattt atatggtcgg ttttttcgct
     2581 accagtggcg agctgacata tgaagtctgg atcggtttct tcgcctctgc gttcaccaaa
     2641 gtgttcaccc tgctggcgct gttttctatc ttgatccatg cctggatcgg catgtggcag
     2701 gtgttgaccg actacgttaa accgctggcc ttgcgcctga tgctgcaact ggtgattgtc
     2761 gttgctctgg tggtttacgt gatttatgga ttcgttgtgg tgtggggtgt gtgatgaaat
     2821 tgccagtcag agaatttgat gcagttgtga ttggtgccgg tggcgcaggt atgcgcgcgg
     2881 cgctgcaaat ttcccagagc ggtcaaacct gtgcgctgct ctctaaagtc ttcccgaccc
     2941 gttcccatac cgtttctgcg caaggcggca ttaccgttgc gctgggtaat acccatgaag
     3001 ataactggga atggcatatg tacgacaccg taaaaggttc ggactatatc ggtgaccagg
     3061 acgcgattga atatatgtgt aaaaccgggc cggaagcgat tctggaactc gaacacatgg
     3121 gcctgccgtt ctcgcgtctt gatgatggtc gtatctatca acgtccgttt ggcggtcagt
     3181 cgaaaaactt cggcggcgag caggcggcac gcactgcggc agcagctgac cgtaccggtc
     3241 acgcactgtt gcataccctt tatcagcaga acctgaaaaa ccacaccact attttctccg
     3301 agtggtatgc gctggatctg gtgaaaaacc aggatggcgc ggtggtgggt tgtactgcac
     3361 tgtgcatcga aactggtgaa gtggtttatt tcaaagcccg cgctaccgtg ctggcgactg
     3421 gcggggcagg gcgtatttat cagtccacca ctaacgccca cattaacact ggcgacggcg
     3481 tcggcatggc tatccgcgca ggcgtaccgg tgcaggatat ggaaatgtgg cagttccacc
     3541 cgaccggtat tgccggtgcg ggcgtactgg tcaccgaagg ttgccgtggt gaaggcggtt
     3601 atctgctgaa caaacatggc gaacgcttta tggagcgtta tgcgccgaac gccaaagacc
     3661 tggcgggccg tgatgtggtg gcgcgttcca tcatgatcga aatccgtgaa ggtcgcggct
     3721 gtgatggtcc gtgggggcca cacgcgaaac tgaaactcga ccacctgggt aaagaagttc
     3781 tcgaatcccg cctgccgggt atcctggaac tctcccgtac cttcgctcac gttgatccgg
     3841 tgaaagagcc gattccggtt atcccaacct gtcactacat gatgggcggt attccgacca
     3901 aagtgaccgg tcaggcgctg acggtgaacg agaaaggcga agatgtggtt gttccgggac
     3961 tgtttgccgt tggtgaaatc gcttgtgtat cggtacacgg cgctaaccgt ctgggcggca
     4021 actcgctgct ggacctggtg gtctttggtc gtgcagcggg tctgcatctg caagagtcta
     4081 tcgccgaaca gggcgcattg cgcgatgcca gcgagtctga tgttgaagca tctctggatc
     4141 gtctgaaccg ctggaacaat aatcgtaacg gtgaagatcc ggtggcgatt cgtaaagcgc
     4201 tgcaagaatg tatgcagcat aacttctcgg tcttccgtga aggtgatgcg atggcgaaag
     4261 ggcttgagca gttgaaagtg atccgcgagc gtctgaaaaa tgcccgtctg gatgacactt
     4321 ccagcgagtt caacacccag cgcgttgagt gcctggaact ggataacctg atggaaacgg
     4381 cgtatgcaac ggctgtttct gccaacttcc gtaccgaaag ccgtggcgca catagccgct
     4441 tcgacttccc ggatcgtgat gatgaaaact ggctgtgcca ctccctgtat ctgccagagt
     4501 cggaatccat gacgcgccga agcgtcaaca tggaaccgaa actgcgcccg gcattcccgc
     4561 cgaagattcg tacttactaa tgcggagaca ggaaaatgag actcgagttt tcaatttatc
     4621 gctataaccc ggatgttgat gatgctccgc gtatgcagga ttacaccctg gaagcggaag
     4681 aaggtcgcga catgatgctg ctggatgcgc ttatccagct gaaagagaaa gatcccagcc
     4741 tgtcgttccg ccgctcctgc cgtgaaggtg tgtgcggttc cgacggtctg aacatgaacg
     4801 gcaagaatgg tctggcctgt attaccccga tttcggcact caaccagccg ggcaagaaga
     4861 ttgtgattcg cccgctgcca ggtttaccgg tgatccgcga tttggtggta gacatgggac
     4921 aattctatgc gcaatatgag aaaattaagc cttacctgtt gaataatgga caaaatccgc
     4981 caactcgcga gcatttacag atgccagagc agcgcgaaaa actcgacggg ctgtatgaat
     5041 gtattctctg cgcatgttgt tcaacctctt gtccgtcttt ctggtggaat cccgataagt
     5101 ttatcggccc ggcaggcttg ttagcggcat atcgtttcct gatcgatagc cgtgataccg
     5161 agactgacag ccgcctcgac ggtttgagcg atgcattcag cgtattccgc tgtcacagca
     5221 tcatgaactg cgtcagtgta tgtccgaagg ggctgaaccc gacgcgcgcc atcggccata
     5281 ttaagtcgat gttgttgcaa cgtaatgcgt aaaccgtagg cctgataaga cgcgcaagcg
     5341 tcgcatcagg caaccagtgc cggatgcggc gtgaacgcct tatccggcct acaagtcatt
     5401 acccgtaggc ctgataagcg cagcgcatca ggcgtaacaa agaaatgcag gaaatcttta
     5461 aaaactgccc ctgacactaa gacagttttt aaaggttcct tcgcgagcca ctacatagac
     5521 aagagctcgc aagtgaaccc cggcacgcac atcactgtgc gtggtagtat ccacggcgaa
     5581 gtaagcataa aaaagatgct taagggatca cgatgcagaa cagcgctttg aaagcctggt
     5641 tggactcttc ttacctctct ggcgcaaacc agagctggat agaacagctc tatgaagact
     5701 tcttaaccga tcctgactcg gttgacgcta actggcgttc gacgttccag cagttacctg
     5761 gtacgggagt caaaccggat caattccact ctcaaacgcg tgaatatttc cgccgcctgg
     5821 cgaaagacgc ttcacgttac tcttcaacga tctccgaccc tgacaccaat gtgaagcagg
     5881 ttaaagtcct gcagctcatt aacgcatacc gcttccgtgg tcaccagcat gcgaatctcg
     5941 atccgctggg actgtggcag caagataaag tggccgatct ggatccgtct ttccacgatc
     6001 tgaccgaagc agacttccag gagaccttca acgtcggttc atttgccagc ggcaaagaaa
     6061 ccatgaaact cggcgagctg ctggaagccc tcaagcaaac ctactgtggc ccgattggtg
     6121 ccgagtatat gcacattacc agcaccgaag aaaaacgctg gatccaacag cgtatcgagt
     6181 ctggtcgcgc gactttcaat agcgaagaga aaaaacgctt cttaagcgaa ctgaccgccg
     6241 ctgaaggcct tgaacgttac ctcggcgcaa aattccctgg cgcaaaacgc ttctcgctgg
     6301 aaggcggtga cgcgttaatc ccgatgctta aagagatgat ccgacacgct ggcaacagcg
     6361 gcacccgcga agtggttctc gggatggcgc accgtggtcg tctgaacgtg ctggtgaacg
     6421 tgctgggtaa aaaaccgcaa gacttgttcg acgagttcgc cggtaaacat aaagaacacc
     6481 tcggcacggg tgacgtgaaa taccacatgg gcttctcgtc tgacttccag accgatggcg
     6541 gcctggtgca cctggcgctg gcgtttaacc cgtctcacct tgagattgta agcccggtag
     6601 ttatcggttc tgttcgtgcc cgtctggaca gacttgatga gccgagcagc aacaaagtgc
     6661 tgccaatcac catccacggt gacgccgcag tgaccgggca gggtgtggtt caggaaactc
     6721 tgaacatgtc gaaagcgcgt ggttatgaag ttggcggtac ggtacgtatc gttatcaaca
     6781 accaggttgg tttcaccacc tctaatccgc tggatgctcg ttctacaccg tactgtactg
     6841 atatcggtaa gatggttcag gcaccgattt tccacgttaa cgcggatgat ccggaagccg
     6901 ttgcctttgt gacccgtctg gcgctcgatt tccgtaacac ctttaaacgt gatgtcttca
     6961 tcgacctggt gtgctaccgc cgtcacggcc acaacgaagc cgacgagccg agcgcaaccc
     7021 agccgctgat gtatcagaaa atcaaaaaac atccgacgcc gcgcaaaatc tatgctgaca
     7081 agctggagca ggaaaaagtg gcgacgctgg aagatgccac cgagatggtt aacctgtacc
     7141 gcgatgcgct ggatgctggc gattgcgtag tggcagagtg gcgtccgatg aacatgcact
     7201 ctttcacctg gtcgccgtat ctcaaccacg aatgggacga agagtacccg aacaaagttg
     7261 agatgaagcg cctgcaggag ctggcgaaac gcatcagcac ggtgccggaa gcagttgaaa
     7321 tgcagtctcg cgttgccaag atttatggcg atcgccaggc gatggctgcc ggtgagaaac
     7381 tgttcgactg gggcggtgcg gaaaacctcg cttacgccac gctggttgac gaaggcattc
     7441 cggttcgcct gtcgggtgaa gactccggtc gcggtacctt cttccaccgc cacgcggtga
     7501 tccacaacca gtctaacggt tccacttaca cgccgttgca acacatccat aacgggcagg
     7561 gcgcgttccg cgtctgggac tccgtactgt ctgaagaagc agtgctggcg tttgaatacg
     7621 gttatgccac cgcagaacca cgcactctga ccatctggga agcgcagttc ggtgacttcg
     7681 ccaacggtgc gcaggtggtt atcgaccagt tcatctcctc tggcgagcag aaatggggcc
     7741 ggatgtgtgg tctggtgatg ttgctgccgc acggttacga agggcagggg ccggagcact
     7801 cctccgcgcg tctggaacgt tatctgcaac tttgtgctga gcaaaacatg caggtgtgcg
     7861 taccgtctac cccggcacag gtttaccaca tgctgcgtcg tcaggcgctg cgcgggatgc
     7921 gtcgtccgct ggtcgtgatg tcgccgaaat ccctgctgcg tcatccgctg gcggtttcca
     7981 gcctcgaaga actggcgaac ggcaccttcc tgccagccat cggtgaaatc gacgagcttg
     8041 atccgaaggg cgtgaagcgc gtagtgatgt gttctggtaa ggtttattac gacctgctgg
     8101 aacagcgtcg taagaacaat caacacgatg tcgccattgt gcgtatcgag caactctacc
     8161 cgttcccgca taaagcgatg caggaagtgt tgcaacagtt tgctcacgtc aaggattttg
     8221 tctggtgcca ggaagagccg ctcaaccagg gcgcatggta ctgcagccag catcatttcc
     8281 gtgaagtgat tccgtttggg gcttctctgc gttatgcagg ccgcccggcc tccgcctctc
     8341 cggcggtagg gtatatgtcc gttcaccaga aacagcaaca agatctggtt aatgacgcgc
     8401 tgaacgtcga ataaataaag gatacacaat gagtagcgta gatattctgg tccctgacct
     8461 gcctgaatcc gtagccgatg ccaccgtcgc aacctggcat aaaaaacccg gcgacgcagt
     8521 cgtacgtgat gaagtgctgg tagaaatcga aactgacaaa gtggtactgg aagtaccggc
     8581 atcagcagac ggcattctgg atgcggttct ggaagatgaa ggtacaacgg taacgtctcg
     8641 tcagatcctt ggtcgcctgc gtgaaggcaa cagcgccggt aaagaaacca gcgccaaatc
     8701 tgaagagaaa gcgtccactc cggcgcaacg ccagcaggca tctctggaag agcaaaacaa
     8761 cgatgcgtta agcccggcga tccgtcgcct gctggctgaa cacaatctcg acgccagcgc
     8821 cattaaaggc accggtgtgg gtggtcgtct gactcgtgaa gatgtggaaa aacatctggc
     8881 gaaagccccg gcgaaagagt ctgctccggc agcggctgct ccggcggcgc aaccggctct
     8941 ggctgcacgt agtgaaaaac gtgtcccgat gactcgcctg cgtaagcgtg tggcagagcg
     9001 tctgctggaa gcgaaaaact ccaccgccat gctgaccacg ttcaacgaag tcaacatgaa
     9061 gccgattatg gatctgcgta agcagtacgg tgaagcgttt gaaaaacgcc acggcatccg
     9121 tctgggcttt atgtccttct acgtgaaagc ggtggttgaa gccctgaaac gttacccgga
     9181 agtgaacgcg tctatcgacg gcgatgacgt ggtttaccac aactatttcg acgtcagcat
     9241 ggcggtttct acgccgcgcg gcctggtgac accggtactg cgtgatgtcg ataccctcgg
     9301 catggcagac atcgagaaga aaatcaaaga gctggcaatc aaaggccgtg acggcaagct
     9361 gaccgttgaa gatctgaccg gtggtaactt caccatcacc aacggtggtg tgttcggttc
     9421 cctgatgtct acgccgatca tcaacccgcc gcagagcgca attctgggta tgcacgctat
     9481 caaagatcgt ccgatggcgg tgaatggtca ggttgagatc ctgccgatga tgtacctggc
     9541 gctgtcctac gatcaccgtc tgatcgatgg tcgcgaatcc gtgggcttcc tggtaacgat
     9601 caaagagttg ctggaagatc cgacgcgtct gctgctggac gtgtagtagt ttaagcttca
     9661 cctgcactgt agaccgacgt ggatagggtg tcggtctacg gtttaaaaga taacgattac
     9721 tgaaggatgg acagaacaca tgaacttaca tgaatatcag gcaaaacaac tttttgcccg
     9781 ctatggctta ccagcaccgg tgggttatgc ctgtactact ccgcgcgaag cagaagaagc
     9841 cgcttcaaaa atcggtgccg gcccgtgggt agtgaaatgt caggttcacg ctggtggccg
     9901 cggtaaagcg ggcggtgtga aagttgtaaa cagcaaagaa gacatccgtg cttttgcaga
     9961 aaactggctc ggcaagcgtc tggtaacgta tcaaacagat gccaatggcc aaccggttaa
    10021 ccagattctg gttgaagcag cgaccgatat cgctaaagag ctgtatctcg gtgccgttgt
    10081 agaccgtagt tcccgtcgtg tggtctttat ggcctccacc gaaggcggcg tggaaatcga
    10141 aaaagtggcg gaagaaactc cgcacctgat ccacaaagtt gcgcttgatc cgctgactgg
    10201 cccgatgccg tatcagggac gcgagctggc gttcaaactg ggtctggaag gtaaactggt
    10261 tcagcagttc accaaaatct tcatgggcct ggcgaccatt ttcctggagc gcgacctggc
    10321 gttgatcgaa atcaacccgc tggtcatcac caaacagggc gatctgattt gcctcgacgg
    10381 caaactgggc gctgacggca acgcactgtt ccgccagcct gatctgcgcg aaatgcgtga
    10441 ccagtcgcag gaagatccgc gtgaagcaca ggctgcacag tgggaactga actacgttgc
    10501 gctggacggt aacatcggtt gtatggttaa cggcgcaggt ctggcgatgg gtacgatgga
    10561 catcgttaaa ctgcacggcg gcgaaccggc taacttcctt gacgttggcg gcggcgcaac
    10621 caaagaacgt gtaaccgaag cgttcaaaat catcctctct gacgacaaag tgaaagccgt
    10681 tctggttaac atcttcggcg gtatcgttcg ttgcgacctg atcgctgacg gtatcatcgg
    10741 cgcggtagca gaagtgggtg ttaacgtacc ggtcgtggta cgtctggaag gtaacaacgc
    10801 cgaactcggc gcgaagaaac tggctgacag cggcctgaat attattgcag caaaaggtct
    10861 gacggatgca gctcagcagg ttgttgccgc agtggagggg aaataatgtc cattttaatt
    10921 gataaaaaca ccaaggttat ctgccagggc tttactggta gccaggggac tttccactca
    10981 gaacaggcca ttgcatacgg cactaaaatg gttggcggcg taaccccagg taaaggcggt
    11041 actacccacc tcggcctgcc ggtgttcaac accgtgcgtg aagctgttgc tgctactggc
    11101 gctaccgctt ctgttatcta cgtaccagca ccgttctgca aagactccat tctggaagct
    11161 attgatgcag gcatcaaact gattatcacc atcactgaag gcatcccgac gctggatatg
    11221 ctgactgtga aagtgaaact ggacgaagcc ggcgttcgta tgatcggccc gaactgcccg
    11281 ggcgttatca ctccgggtga atgcaaaatc ggtatccagc cgggtcacat tcacaaaccg
    11341 ggtaaagtgg gtatcgtttc ccgttccggt acactgacct atgaagcggt taaacagacc
    11401 acggattacg gtttcggtca gtcgacctgt gttggtatcg gcggtgaccc gatccctggc
    11461 tctaacttta tcgacattct cgaaatgttc gaaaaagatc cgcagaccga agcgatcgta
    11521 atgatcggtg agatcggcgg tagcgctgaa gaagaagcgg cagcgtacat caaagagcat
    11581 gttaccaagc cagttgtggg ttacatcgct ggtgtgactg cgccgaaagg caaacgtatg
    11641 ggccacgcgg gtgccatcat tgccggtggg aaagggactg cggatgagaa attcgctgct
    11701 ctggaagccg caggcgtgaa aaccgttcgc agcctggcgg atatcggtga agcactgaaa
    11761 actgttctga aataaatatc tgtaataaga aatagccctc gccgcttccc tctacaggaa
    11821 tggcgaaggg ctgtcggttt cgacatggtt ggccatcgta tgatggcctt ttttgtgctt
    11881 atcgcgatga ttttcgttgc gctatcaggg taaatttata gtcatcggta ttaaaagcgt
    11941 tgcggctata ttcaaacacc cgaccatcaa ctaaatatcc acgcgatact ttttcaagaa
    12001 tcggctttgt ctggctgata ttaagcagac ggctcatctc ttcggttggc atcagaggaa
    12061 tgatttcctg ttcgctacga tcgataacca ttttcttcac ttcttcaata aagtgatatt
    12121 ttgaattttc catgacctgc caggtgagat ccgggaacaa cgcaagcggc atccaggttt
    12181 cttccagcgc cattggcttt tgcttgcgat agcgcacgcg cttcacatgc cacacacgat
    12241 cctgcgcggt gatttgtagc tgttgctgaa gaaaatcgtc agccggaatc acttcaaata
    12301 tcaggacttc actgtgtgta tcgacgtgac ggtccgacag tttttcatca aaactggtta
    12361 actgaaaaat atcgtaattg acccgctctt ctttgacgta agtcccgctg ccctgaatgc
    12421 tttcgaggat ctgttgctcg actaactggc gcaaagcctg acgcaccgta acccggctga
    12481 cgccaaactc tgtttgtaat gccgattcgg taggtaacgc atcgccaggt ttaagctcgc
    12541 cacgcgcaat ttgttcacga atgcgatcgg caatctgccg gtataagggc ttgtgtccca
    12601 tttttagtat ctcattaata cg
//
LOCUS       DAFNBJ010000138        12484 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000138 DAFNBJ010000000
VERSION     DAFNBJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.138"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            217..1047
                     /locus_tag="L2X49_003740"
     CDS             217..1047
                     /locus_tag="L2X49_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HBN4798761.1"
                     /translation="MSMPLSNALQSQIITDAHFLHHPIVDSEFIRKLKYARMDSENIY
                     LPPLTRGNNHNYDGKSVVEIRKLDISKEPWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHATTELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYLGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1188..1961)
                     /gene="uxuR"
                     /locus_tag="L2X49_003741"
     CDS             complement(1188..1961)
                     /gene="uxuR"
                     /locus_tag="L2X49_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBN4798762.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2176..3636)
                     /locus_tag="L2X49_003742"
     CDS             complement(2176..3636)
                     /locus_tag="L2X49_003742"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBN4798763.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3717..4901)
                     /gene="uxuA"
                     /locus_tag="L2X49_003743"
     CDS             complement(3717..4901)
                     /gene="uxuA"
                     /locus_tag="L2X49_003743"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBN4798764.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5353..5445
                     /locus_tag="L2X49_003744"
                     /pseudo
     CDS             5353..5445
                     /locus_tag="L2X49_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5688..6590)
                     /gene="fimH"
                     /locus_tag="L2X49_003745"
     CDS             complement(5688..6590)
                     /gene="fimH"
                     /locus_tag="L2X49_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBN4798765.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGSVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTVDAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6610..7113)
                     /gene="fimG"
                     /locus_tag="L2X49_003746"
     CDS             complement(6610..7113)
                     /gene="fimG"
                     /locus_tag="L2X49_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBN4798766.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7126..7656)
                     /gene="fimF"
                     /locus_tag="L2X49_003747"
     CDS             complement(7126..7656)
                     /gene="fimF"
                     /locus_tag="L2X49_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBN4798767.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7666..10302)
                     /gene="fimD"
                     /locus_tag="L2X49_003748"
     CDS             complement(7666..10302)
                     /gene="fimD"
                     /locus_tag="L2X49_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBN4798768.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10369..11094)
                     /gene="fimC"
                     /locus_tag="L2X49_003749"
     CDS             complement(10369..11094)
                     /gene="fimC"
                     /locus_tag="L2X49_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBN4798769.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11131..11670)
                     /locus_tag="L2X49_003750"
     CDS             complement(11131..11670)
                     /locus_tag="L2X49_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBN4798770.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11735..12283)
                     /gene="fimA"
                     /locus_tag="L2X49_003751"
     CDS             complement(11735..12283)
                     /gene="fimA"
                     /locus_tag="L2X49_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBN4798771.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3103 a         3230 c         2947 g         3204 t
ORIGIN      
        1 aattagtaat aagatatgct tttaaattgc atgcaaataa ttttaaatga gcgttttttg
       61 atactttaga gtgtttccta ccctttaata tcaccgtgta gagttgatat cactacaaca
      121 gaaacatgca tgtcatttgt ttacttctct ttttactaca gaaaaattta tctatttttc
      181 taaacatcag tgaaatactc ataaaaggta actattatgt ctatgccatt aagcaatgcc
      241 cttcagagtc aaattattac agatgctcac ttccttcatc acccaatagt cgacagtgag
      301 tttatacgta aattaaaata tgcacgcatg gattcggaaa acatctatct tccgcctctt
      361 actcgtggca ataaccataa ttacgatgga aaaagcgttg tcgaaattag aaaacttgat
      421 attagtaaag agccctggcc atttaattat gtgacaggag catgcagaga atccgatggt
      481 attacaacaa caggacgtat gctctatcgg aacttgaaaa tcacctcagc tttagacgaa
      541 atatatggtg gcatatgtaa aaaggcgcat gctaccacag agttagcaga aggtttacgg
      601 ctaaatttat ttatgaagag ccctttcgat cctgttgagg actatacagt tcatgaaata
      661 acattaggcc ctggttgcaa tgttcctggt tatgccggaa ccacgatagg atatatttca
      721 acccttccag cttctcaggc aaaaagatgg accaatgagc agccaaggat tgatatctat
      781 attgatcaga taatgacggt aacaggggta gcaaattctt ccggattcgc ccttgccgca
      841 ttacttaatg ctaatattga gttgggtaat gacccaataa ttggtataga agcctatctc
      901 ggtaccgcgg agatccatgc aaaaatggga tataaagtta tcccgggtga tgaaaatgca
      961 ccactcaaac gtatgactct gcaaccgtca tctcttccag aactgtttga attaaaaaat
     1021 ggtgaatgga attatattgg taaataacta ccaggaagaa tgagtactaa cattcaggtt
     1081 atacagatag ctaaacaata ccctgcaatt tacaaagtaa tactcattgc gacttcttaa
     1141 tttaagaaag cgcaaggaac gtttaccctt gcgcttatta taataagtca ggcgtcgact
     1201 ttatccagcg gccatgaatc aaacagatag ccatcaaaat aaatatcgtc aacgttcgag
     1261 aattccagca gacgttgctt aacgttttcc agatgctgcc acattgccag cttcgccgct
     1321 cgggcatctt ttttgattaa cgcggcgagg atctgtttgt gatcgcccaa ccactctttg
     1381 cgatacaggc tgtcatccag atggctgtgc aactggatcc acattggatt gttttcccgc
     1441 cactgccagg actgacggaa cagctccacc agcatgctgt tatgcgttgc ttcggcaatg
     1501 gcgagatgga actgcatatc accgctttcg ctgctacccg gcgcgctgga agccagttca
     1561 cgctcttcca gttgcaatgc ctgacgcatt ttgacgatat cttcgcgggt agcctgcagg
     1621 gcggcaaact cggcgatgtt actttccagt aactgacgcg cctgtaacag ctcaaaagga
     1681 cccgcatcat tgcagacgtt ggcatccgga ctgtctgtgt tgtggctgcc tgagttatca
     1741 agaacataga taccggcacc ccggcgtact tccaccagcc ctttgatctc cagcatgatc
     1801 agcgcttcac gtaccaccgt ccgcgtgaca tcaagcattt ctgcaatttc acgctccggc
     1861 ggcagccgtt cgccagggtt gtacggcgtc tttatgatca gatcgcggat catcgccccg
     1921 acttcctggt aaggtctttg cgcagaggtg gcagatttca tggtgttgtg tccgttaggt
     1981 gcggttaatc aaatttttgc cggatgcggc gtgaacgcct tatccggcct acaacgggca
     2041 attttacggc ccctgttaat ctcaaactat accattagca ctggaagttg gcacgtacag
     2101 gtgggcaaaa aaacgaaaac tggtcaacca aatatcagtt tgattgacca gtaagtctgt
     2161 agggtaatca gttagttaca gcgcagccac acactcgcgc gcaccgcgtt cgcacagctg
     2221 ctgatatgcc gccgtcaccg cgccaacaaa gtcggcattc tgcggcagat catcggcaaa
     2281 aataccgctc aggcctagca gcgctttcac gcggtctgcg ccctgatact gcgcgttaat
     2341 cttctggaac tcggccagca tcgggtcgac cacgtcaatg gcattaccct gctcatccac
     2401 gccctgggtg taacgcatcc agccagccac gcccagcgcc aggtgacgcc agctgccgcc
     2461 gttttgcagg tgcagacgca ccgggtccag cagacgctgc ggtaacttct ggctgccgtc
     2521 catcgcaatc tgccaggtac ggtgacgcag agacgggttg ctgaaacgct cgatcagcag
     2581 cgtcgcatag gcgttcaggt ccgtaccttc cggcatcgac agcgttggcg cttgttcctg
     2641 catcatcagg gcaaaggccg ctttgcgata atccgggtta gtcatggtgt cggcaatagt
     2701 ttcatagccg ccgaggtaac cgaggtacgc caggaaagag tggctaccgt tcagcatacg
     2761 cagcttcatc atttcgaacg gcacaacgtc tgcaacgaac tgtgcgccca ctttatccca
     2821 gtccgggcga ccattaacga aattatcttc aatcacccac tgacggaacg gttcgcaggc
     2881 aatggcgcac gggtcgtaaa cgcccagctg gtcagcaatt tcctgtaagg tttctggcgt
     2941 cgccgccgga acgatgcggt caaccatggt gcacgggaag gtgacgttct cttcaatcca
     3001 tgccgccagc tgcgggtcac gcgcctgagc cagtcccagt accgcgacct tcgccacatg
     3061 gccgttttca cgcacgttat cgcaggacat caccgtaaac gctttcaacc ctttttcacg
     3121 acgcagccgc agggcttcga cgatgtaacc aatcgcggac ttcggcgcag tcgggttttc
     3181 cagatcgtgc ttgatcagtg ggttattgag atccagctga ccgcttgccg catcagcgca
     3241 gtagcctttt tccgtgactg tcagagagac aatcgccgtc tgcggacgcg ccatcgcatt
     3301 gagaatacct tcgcagccgt cgatttccgg atgcagcgct tctttcatcg aaccgataat
     3361 tttcagctcg gtgctttccg cgcctttttc cgccacggtg tacagcagtt gctgtttttt
     3421 caggttttcg atcagcacgc ggtcgttgcc tggcatcagg ttaacttcgc agatacccca
     3481 gtcgctgtcg gtgctttcca gcaggtgatg ggtatacagc gcctggtgcg cgcggtgaaa
     3541 cgccccgcaa ccgagatgca caatgcgtga ttccagacga gaatgctccc atgacgggcg
     3601 ggcaaccggc agattgctgt caacaatagt agtcatcgta aactccagaa tccgtcttca
     3661 gagagtgacg gcaaggaaga aggaaccggc acggcgcgca gccatgccgg tgggtattaa
     3721 cggctaaaga aagcgcgctg gatcgccagt tcgacaccgc gaacttcggc caggcctttc
     3781 agacgaccaa ttgcggagta acctgggttg gttttcttct tcaggtcgtc cagcatctga
     3841 tgaccgtggt ccggacgcat cgggatcagg tcttctttgc cttccgcttt acgacggtgt
     3901 tcttcttcaa caatcgcttt caccacttcg tacatatcaa cgtcaccgtt caggtgcgcc
     3961 gcttcgtgga aggttttcgg gttatcttca cgcatggtgg agcgcagatg ggtgaagtaa
     4021 atacgcggac cgaactgctt gatcatatca accagatcgt tgtcagcacg cacgccgtag
     4081 gaaccggtgc acatggtgaa accgttcgcc atgctgttta cggtatcaac catccactgc
     4141 atatcttcaa tggtggaaac aatgcgcggc aggccaagga tcgggcgcgg cggatcgtcc
     4201 gggtgaaccg ccatacgcac gccaacttct tcagcaactg gaataatcgc tttcaggaag
     4261 acggcaaagt tttcgcgcag cttcgcttta tcgatatctt tgtacagctc caggtgctta
     4321 cggaactggt cgagggtata cccttcttcc gcgcccggca gaccggcaat gatgttacgc
     4381 gtcagacgcg ctttgtcttc gtcgctcata gtggcgaagc gaacagcagc ctgagcaatt
     4441 tcttcttcgg tgtaatccgc ttccgcgcct ggacgtttca ggatatgcat ttcgaatgca
     4501 gcgaattcga tctggtcgaa gcgcagagct ttggagccgt ctggcagcac gtattcgagg
     4561 tcagtacggg tccagtcgag caccggcatg aagttgtagc acacggtgcg aatgccgcac
     4621 tgcgccaggt tgcgcagagt ctgctgatag ttagcgatcc actgctcata gttgccagtg
     4681 tgggttttga tatcttcgtg aattggtacg ctttctacga cagaccacac caggcctgcg
     4741 tcttcaacga tcgccttgcg tttgaggatc tcttctacgg accatacttc gccgttcggg
     4801 atatggtgca gcgcggtaac cacgccagtt gcgcccgcct gacggacatc agctaaagaa
     4861 accggatcgt ttgggccgta ccagcgccag gtctgttcca tgatttttcc tctttcacat
     4921 agtgggttcc acagcaaaga gacgcggaac cctggagttg tttaggtatc cggatgaaca
     4981 accagaccgc agccgtggcg ttgcatcgga tgagatggcg tataagttct accttttggt
     5041 aagacatgtc aacccgaatt ctgaaattgg ttaaccacat cacaagaatt tcactctcat
     5101 ggttgttgct gcaaattgca gcaaagggta atcgatggtt attgatcaga ggatgtgaca
     5161 ttcatcgcaa caatggttga ccaatttaca taacatgtcg cccaaaataa caacggttca
     5221 accaggtaag gtaaaacgtt atgcatgtgc ttaacattct ctgggtggta ttcggcattg
     5281 gcctgatgct ggtactgaca aagaccgcag gttcagacac attcgctgca ttagcaatgc
     5341 ctcttagata attttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag
     5401 ctggtcaact ccgtggttgg gctgattatt agcaaggtag cgtaaaagaa caccagagcc
     5461 tgccaatggc aggctctgac tgatgacaaa gtctaaaaat gtgcccggac agtcccctcg
     5521 cccctccggg gagagggtta gggtgagggg aacagaccag caaagtgcgc tttgtttatg
     5581 ccagatgcga cgctgacgcg tcttatctgg cctacaaagg gctaacgtgc aggtttttag
     5641 cttcaggtaa tattgcgtaa ctgcattagc aatgccctgt gatttcttta ttgataaaca
     5701 aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc ggtacgtgcg
     5761 taatttgccg ttaatcccag acttaccgcc gaagtcccta ctgctcctaa cgataccgtg
     5821 ttattcgctg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc ctgcgctggt
     5881 gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctacggttgt gccggagagg
     5941 taatacccca gattttggct tttcgcacaa taaacggtaa gaggaattgg cactgaacca
     6001 gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcgcagcc gccagtaggc
     6061 accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc gctgttatag
     6121 ttgttggtct gtcgcaaaat aagcacggca attaatgagc cagctttaat cgccaccccg
     6181 cccgcactgc tcacaggcgt caaataaagc gccaccggcc acggcttatc cgttctcgaa
     6241 ttataaacaa cgcgcggcgt ttcgctggtg gtaggaaatg gatagctact gccactatat
     6301 tttacggtcc cggaaaaatt agataacacg ctgccataag ccgagcctcg ttgcagtgtg
     6361 acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt cgaaagatcc
     6421 acgaccaggt tttgccccac attcacggcg ggcgcaaggt ttacataaac attggcgctg
     6481 ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga ccaggcattt
     6541 accgaccagc ccatcagcag tacagcaaac agggtaataa ctcgtttcat tacaatcatc
     6601 tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg ttccctgagt
     6661 ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg attgtgagga
     6721 atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac tgtcatcctg
     6781 tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg tactgtcggc
     6841 tgccccgctg aagctggcgg tgaccctcga cgttcccacc ggacaattag tcaactcaag
     6901 cgcaacatca tgccaggccg atgccgcccc ggcagacatc agactgaaag aataaagatc
     6961 gccgagatca accgtggcat tggtggtgga aaccgtacac ggtttggcga cgaccttacc
     7021 gttcaccgtg atggtgacat cggctgcctg tatcgtcgca cttgcgagcg ccaatattgc
     7081 cgccaataaa tacccacgtt tgcgccattt catgagcatc tccagttact gatattcaag
     7141 agtgaaggta gccgtggcat tgatatgccc cgcagtgaca ggcacctgtg tcgccattag
     7201 ccgggcgtaa aaattcaacg tatttggttt acccggcgtc agggtcgtcc acgaaatcgc
     7261 ggacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct gtattcccag
     7321 tcccgaagcc gctgacaccg tattttcaag tgcaagcaga ttggcattgt ggctatctgc
     7381 aacgccggta aacccaactt ttacggcaga aacggcatta ccacagggtg acagcaaaat
     7441 acgaaatgga acaacaggag tcgtcgcgcc aatgttgtta aattgcttcg ccgcgttttc
     7501 catcagatca acagtaaaat tggttgattc agcggccaca ctacagccgt tatctctgac
     7561 atagccgcgg atagtaatcg tgctatccgc agccaaagcg tgactcaccg ccagccacaa
     7621 aaaagcgcac agaagataaa aaggtttgtt tctcatcacg cccccttaac gacattcagc
     7681 tgatagctgg gttaataact gctgctgact ctctggtggc agttgataat tggcgacaca
     7741 gtgagcattt tcctcttctc cccatttcac ctgaactttt cccgctaaag gcattccgct
     7801 gaggtaaacc tgaccattat ccgcaacaat gccgctactc tggctactct ctgatgtcac
     7861 catcgcccca aacggcagcg gcttattatt gtgggttagc gtcatgagca gttttatccc
     7921 aacgcgcgct ttaaactctg ctcgcacgat cgccccacga gtgggaacaa cgttagcgac
     7981 cgcgttatct aaatcgacgt tatcagccag ggtattggta tccagcgcca ctctgttttc
     8041 cctatattca gtggcataag gcagcacggc ataaccgcgc cagtcggtac gcacccccgt
     8101 ctggttttcg acttttgcat cttttgcgcc aggcgcttta acaagcacca ccgtatcgtt
     8161 taacggctgc cccagcgtta cgccattggc atgagccaat accccaccgc tgactccgta
     8221 atagagctgc ttaatatcat cgctatggct gtaaccgata ttggcattgc cgtaaccacc
     8281 gcgataattc agcgtggcgt agcctgtgct tccgctatta ccatcgcctc ccccggcata
     8341 gccggtttgc acgctatagc tgaggttgtt gtcttccagc aacgtaccgt atacaccagc
     8401 cagattggtc atccgaccgt tgagatcgtg tgacatgctg tagctggcac tggcatgtcg
     8461 ccactgagat ttactgtcag aacgcagcca gtggctgaaa ggaatattga cgttaagcgc
     8521 taacatctga tcacgtcctt tttgccaggc gtttttcgtc aggctatagc tgagcgtcca
     8581 gttgatatct tcgaacgcag tatttaatcc agcctggaat tgctcatcga cattactcgt
     8641 tccccaataa gtttgatggc taccactcaa atacagtgtt gatgtgcgcc cgagttgctg
     8701 agtaacggtg agctgtaatt tcccgcgttt gttataagcg aggttgtaat agtcggtgaa
     8761 tttcggctta acctgaataa ctccatcctg tgtttcgatg ttgtagccat tcattcgact
     8821 gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa taacggtaac ccactaactg
     8881 aatattcgtg cctgattcat tgagcgattt gttatagaga aaacgcaccg attgtccgtc
     8941 atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc atatccacag acagagcgcc
     9001 cagtgccccc atgtttttcc cgataccgaa attaaaagca cgataacgat ccgccagttg
     9061 cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac tctggaaaaa
     9121 gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa tggaataacg
     9181 agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa aaatctgcgt
     9241 gctgccgtca gcctctttga tcgttacctg caagtcacca ctattacctg cggcatagat
     9301 atcgttgatg gtaaaaggcc ccggtggcac cgtactatta taaatgtcat acccattttg
     9361 tttaatagtg acctgtgcag taccacgagc aataccgtgg atcaccgggg caaatcctct
     9421 ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa agttaatacc
     9481 atcgaaaata tcgccctgag tataaccatc acccagcgtc agccgggaac gtaacggtat
     9541 tatgtctcgc tcaagccagg tattgatatg ctgccattta tttttgctac ctgatgatct
     9601 gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac caatatttaa
     9661 cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccgat tctgtacact
     9721 atttccgctg aaattataat tgagcaatcc ggcattaata ccgggatccc ataactcagg
     9781 aggaatataa ccacgcgcgc gattactcat aaatgcctga gggatcgtca ggttcagtcg
     9841 ctgctgacca acatccagat gcgcagtagc gtcctggacc attgtggtta atggcacaca
     9901 ggcatcatcc gccagcagat tcataccggc gacagaagcc gtattcagcc ccatactggc
     9961 gagttgcgcg cgtgtcaggc agggaacaat cccttgttca ctgtcgcccg tattaaatgt
    10021 gacatcacgc gttgccatat aaccattatt caaatagata tcgacgcgat acgtccctgg
    10081 cggtaattct tgcccatttt caaaacgcga taaatcggcc acagcctggg gatcatccgc
    10141 taaaaaacgc gggttaaaat agagttcggc agatgacaaa ggtgcctgtg cggcaaaagc
    10201 acaggcaaca aagagccgga caaaaaaacc agccaaacga tgcttacgaa tatgcaagca
    10261 ttgtgtgttt cgctggtaaa gtcttaaatt cagatatgac attattcctc cggtttaaag
    10321 tcacccggca gtcaattctt tttattcagg cgaaaaattc cccctgcgtt attccattac
    10381 gcccgtcatt ttgggggtaa gtgcaccata atcatttatt gttcggtaag taatattgct
    10441 tcccgcatca gaaggcaatt taaccgtgct ttcgcccatt ggaggcacca aggcattttc
    10501 aagaacccgg gttccggcat tcaactctgt taccgtcagg taatagggtg tcgggttaat
    10561 cagcgtcaga gaattcgcgc tacgacgaaa tcttaatttt tctgcggcct gatcgggtgg
    10621 caacgctaat ttagccgggc gatagtacag tttaatgcgg ctgataattg cgagctgtag
    10681 cgtattctca gtcaattttg atttatccat tgacggaatc gctttaacat tcatccagaa
    10741 taaactttcc cgatcctgtg gcaattggtt atttgttgca tcaagaatac gcaaggtatt
    10801 ctcttttttt cccttcatcg caaacagagg aggcgtcacg ataaaacgac catcctttac
    10861 accatcggca ttttccaccc atgattgaat taaataggta ctattttcat cattatttgt
    10921 cacggcaagt tgcacttgtt tttgccctgc cggataaatt acgcgagtcg cacctaaggc
    10981 cactcccgct tcagcgcgtc cggcaaccat cattgccata aacatcagga tacctgccag
    11041 caagcagaat gttatttcct gcgatttcct tacattgacg tttttattac tcactgtcct
    11101 gttcctgtta tcacattatc tgctgaacaa ttactgatag gttaaagaga accaggcctg
    11161 ggcattggcg atgccgccag taacccgacg cccggtagca cgatatttgg cgatgaaatg
    11221 tagcgaagta gagcctgagt aaagccgttt ccagtttgct ggaggacgat taatcggtac
    11281 gaggtttcct tcatcatcaa acaacgctac gccaatattg gtggctatcc ctggcccctc
    11341 tcccacggaa agcacatccg gatttttacc atccgcgaca ccgtgaaacg ccacccccac
    11401 acgttcactc accaccgtgc tacattcccg taaatgaata acaaaaggca ccggtgcgct
    11461 atcttcccct gccgcatgaa accggttact gctgatttgc cccatattga ccgtcatttg
    11521 tttatcaccg gcttcaatcc ggcaagtttc cgcaataatg acgccctgaa attgcatatt
    11581 tccgccgggc agcgtggtat tccatttatt tccggccaga gcaaacattg gcagcaacga
    11641 cgctaacaga aataatctct tccttttcat cgttattttt atcgcacaag ggtgggcatc
    11701 cctgcccgta atgacgtcct tgaacctggg taggttattg atactgaacc ttgaaggttg
    11761 catccgcatt agctgcaccc ggggttgcct cgccgattgc ataataacgc gcctggaacg
    11821 gaatggtgtt ggtaccgtta ttcagggttg tttgtgcact aaatgtcgca ccgtccagcg
    11881 tcagagcatt gcctgttctg tccaggatct gcacgccaac gtttgttgca ctacctgcag
    11941 cagaactctg cagagccagt acatcagtac gcgtagcatc aattgccgta cctaagaagg
    12001 caacagcggc ttttgtggca acagtggtat cgcaatcatt caactgaatg ttaaaaccaa
    12061 cggcagagct ggttgctcca gcctgcttca ggctagcggt acgaacctgt cccaactgaa
    12121 cggtttgatc aacagagcct gcatcaactg cgcaagcggc gttaacaact tcccctttaa
    12181 agtgaacggt cccaccattt accgtcgtag tatcggccag agccgctgcg gaactgaggg
    12241 acagagccga cagaacaacg attgccagag ttttaatttt catgctgctt tcctttaaaa
    12301 aaactatttc taaatcgaca tgggcagtcg ttctgtacac tttgttttgt caacgagttt
    12361 gactgccaac actgcacagt tttcccccaa aagatgaaac atttggggcc attttgactc
    12421 atagaggaaa gcatcgcgca caaacttttt cagtttattt gttggcttaa tattctattg
    12481 ttat
//
LOCUS       DAFNBJ010000139        12374 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000139 DAFNBJ010000000
VERSION     DAFNBJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.139"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..550)
                     /locus_tag="L2X49_003752"
     CDS             complement(<1..550)
                     /locus_tag="L2X49_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBN4798772.1"
                     /translation="MALLGYARVSTSHQKLTSQITELNTAGVRDDRVFTDMMSGATDE
                     REGLQRLLARAEKDDIILCTKMDRLGRNTADMIRIVDTCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRQAAMIKGVKFGRKPHKKAELANELINKDVQ
                     VKEVMEKTGISRATYFRLKRKTV"
     gene            709..3717
                     /locus_tag="L2X49_003753"
     CDS             709..3717
                     /locus_tag="L2X49_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBN4798773.1"
                     /translation="MAADFLTDKQTQNYGRYAAEPNEIQLARYFHLDERDLTFINLRR
                     GRHNRLGIALQLTTARFLGTFLPDLMQIPPGVQFYVARQLNIRYPEIISRYAQRENTR
                     WEHHGLIRQHYSYHDFGDFPWSFRLKRLLYTRAWLSNERPGLMFDFATAWLLQNKVLL
                     PAASTLTRVIGEIRERATRRLWRKLAALPNRWQAAQLAGLLEIPEGQRLSVMEHLKRG
                     PVTISGPAFTEALERYTRLRSLEFSCLNFTGLPAIQLRNLARYAGMASVKYISRMPEE
                     RRLAILTAFVKAQEISALDEAVDVLDMLILNITREAKKTGQKKRLRTLKDLDRAALLL
                     ARACALLLDEDTGDDLLRKTIFSSVPVARLAESVEKVNELARPQDTNFQDEMVEQYGR
                     VRRFLPALLRDLHFRAAPDGEHTLAAIHYLAELNGSKKRILDDAPEHIISGPWKRLVY
                     DAEGRIQRAGYSLCLLERLQDALRRRDIWLENSDRWGDPRQKLLQGEEWQAQRVPVCR
                     ALGHPTNGSKASVQLAARLDETWKTVASRFDRNTAVDICNEGKHPSLTISSLDKLDEP
                     PALIRLSSRVRQLLPPVDLTELLLEIDARTGFTREFSHVSESGARAQDLHISLCAVML
                     AEACNIGHEPLIKHNIPALTRHRLSWVKQNYIRAETLVSANARLVDFQSSLALAGYWG
                     GGEVASADGMRFVTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGFHGIVIPGTLRD
                     SIFVLEGLLEQQTGLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLADAGEAVFWRAD
                     KAANYGALDELARGCVDLSKIESHWDEMMRVAGSLKLGTIHASELIRSLLRSTRPSGL
                     AQAIMEVGRVNKTLYLLNYIDDEDYRRRILTQLNRGEGRHAVARAICYGQRGEIRKRY
                     REGQEDQLGALGLVTNAVVLWNTLYMQEALSHLRSTGEGPEDEHIARLSPLMHGHINM
                     LGHYTFTLPEDIMKGELRPLNLNLNNELPP"
     gene            complement(3940..4608)
                     /locus_tag="L2X49_003754"
     CDS             complement(3940..4608)
                     /locus_tag="L2X49_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798774.1"
                     /translation="MAASRITHLITSCTKGKHSQCGSMPELSIRSGQTPEEAMSSWAA
                     TIKRSQSASPVPALSLYAGNHWSTAKEILRTTENLELWVISAGLGFLNSRDLVDAYEA
                     TFHDLPFSHRQWWRELTNTFGKERTAKSIETLMAARPFDDYVIAASPVYIEATEDDIL
                     AGASKLNNHIAQLTVVTSGEYSGLLESYLIRSESRMMRQLSSNMVCLNIKLAQYIIGS
                     RRYS"
     gene            5117..5929
                     /locus_tag="L2X49_003755"
     CDS             5117..5929
                     /locus_tag="L2X49_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBN4798775.1"
                     /translation="MSMNYQPELKLSTRTEQLASSRDAAMQKFLDGIMLIAEASAICG
                     FSLFNSKIMAPNAFGLPASLAASIEEGRQQIDRKTWNNLFEETGIDRFWNHNQRAEFS
                     ESLRSAPPIASLTVIRSTLRQAVAIRSITLAQGFVDLLCQLDRRYKTNAQQFVMPKKL
                     VLRGIFPDSNMMRYNGFSQDNLFYLNDFENIVCICSNAATPPVGSGLNMYDRLAALRK
                     TDFTGDITDPKGWKCRLFVNGNVHISVECESLHNTLNDLISIYFANQIPAKG"
     gene            5935..7152
                     /locus_tag="L2X49_003756"
     CDS             5935..7152
                     /locus_tag="L2X49_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798776.1"
                     /translation="MTDNENLMNGDEQTASNLSEETTKKRPLSPELLKNSPYSSIAKV
                     KYFTEIEQICLDKGISTENLDQFFKAHWLRDKMGGSFARAQEMLAAYKQYVDEVPEEA
                     RAIEIPDQIKEAFSDFTAFIIWYFRLSYTAIQSDSVKIAKAEITQLRHRNAEILEELS
                     QSKEQVTVLNNEKVNLINQLEQQRELASKLGDSLREAEETLATTQSELQYAQNEIQLI
                     QQTVGTLNQQLSERKQELVSQQEYQKQLNDENKAQQVELTALKSQNDHLQRTVSDLKV
                     SVSQLEQDLSSVQTHSSELSSSLAEKDTTLTLVRSELSTAKGENDQLRAEAQRLADES
                     LVAKKVQTDQTEELQQLRNQMISMEATLNAEKTIAESLRGTIKQLTEAMTGAVAIKPK
                     SSGASKPRNKKTT"
     gene            complement(7207..7410)
                     /locus_tag="L2X49_003757"
     CDS             complement(7207..7410)
                     /locus_tag="L2X49_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798777.1"
                     /translation="MAYILSMSEQVKLKAFLAAVLDDYKLGAISQQQAVGGITSLVDA
                     IEISDSGKISLMLSEGRKLLRTG"
     gene            complement(7424..8743)
                     /locus_tag="L2X49_003758"
     CDS             complement(7424..8743)
                     /locus_tag="L2X49_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1173 family protein"
                     /protein_id="HBN4798778.1"
                     /translation="MGQKFAVFIAYDDEPNTKRYSAEFQTQDEYVKGWQSALKKAHHT
                     SGQKSVISCGCRGKGAKRLYVRSLPNSDTFILIKAANTGTEHDPSCVFFDLDARHTGL
                     KGYASNVVRINNEGTMSIRLGIGMTEKDPPEKSEVPSLPQIQRPEGGQASMTLSGLLS
                     LLWTEAGLNVWYPNMAGKRNDSLVRYRMLEAAKQIRSGRACIGDHLFIGVADSKSKVA
                     SEQVQLLSSAELSDKRLLLLSVLPRYDAEKHEKPLKFLPMRNFGGMPLTFFNSDGHWD
                     SVKRRFPLEYAAWKNGGKVVVFALTSPVSVTSRGISARAHQIVLMLVSDNWIPLDSSY
                     EAIVSRKLDAEHRHYVKPMRYDASASDVFPDFYLLDTRSDKPFPMEVFGMSTPAYLAR
                     KELKKAYYNQEYGAYGWWHWDATEKTEYQELPHFPEARRYNSPESQP"
     gene            complement(8768..8938)
                     /locus_tag="L2X49_003759"
     CDS             complement(8768..8938)
                     /locus_tag="L2X49_003759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798779.1"
                     /translation="MMTYFDSAEDLTISKQRALQELAKHGVVASDIDVFFSELGEREE
                     YNAQEVLIWLGY"
     gene            complement(9067..10458)
                     /locus_tag="L2X49_003760"
     CDS             complement(9067..10458)
                     /locus_tag="L2X49_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBN4798780.1"
                     /translation="MLNDETHSSIHKLLVQVASIYDVKDLAAQLNAATGSDWSRASLI
                     RQIKGSVNECRITQEEYHYLRSLLPSRPADYDQKFFRFIDLFAGIGGLRSGFDAIGGK
                     CVFTSEWNQFSRRTYSANWYCEDTEHHFNSDIRDITLSNLPDVSEDQAYASIAASIPD
                     HDVLLAGFPCQPFSIAGVSKKNSLGRKHGFECDTQGTLFFDVARIIRAKQPAIFVLEN
                     VKNLKSHDKGNTFRIIMKALDELGYDVADCDAHGSKDPKIIDGKNFRPQHRERIVLVG
                     FRRDLKLHDGFSLSGVSALYPSRRPTLGELLDAEVDSRYILSPKLWEYLYNYAKKHQA
                     KGNGFGFGLVNPLDKSVVCRTLSARYYRDGSEILLDRGWDHSIGEKDFNDPVNMARRP
                     RRLTPRECARLMGFEQPGKVTFRIPVSDTQAYRQFGNSVIVDVFAAVASLLRSRIEIA
                     VAARLKGEFEAAS"
     gene            10717..11232
                     /locus_tag="L2X49_003761"
     CDS             10717..11232
                     /locus_tag="L2X49_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBN4798781.1"
                     /translation="MTLLKKLLLPTLIVLFPAAVLAGQSSFEAKVVKIIDGDTITALD
                     GQNTSIKIRMYGIDAPESKQAFGQKAKQALSSAIAAQNITVIDHGPDIYGRMLGTIWL
                     DGYDINASMVDSGYAWVYRFDGNPIVPNYLKFEASAQKAVKGLWVDPNPVAPWEWRQQ
                     NQKPQKTKSRG"
     gene            11238..12170
                     /locus_tag="L2X49_003762"
     CDS             11238..12170
                     /locus_tag="L2X49_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798782.1"
                     /translation="MTSQHPLLKIALNGDGLLSWPNSPGQTMRCPTCMRDVITGPDGD
                     LIHQMEEGANPCTPSVDVVISKAIIELLSTGERLYVNPVKNANRTLAPSFIFLHKDKQ
                     LRPFINTEYQPAGATWRSDKGNRLGFFYMDARASKINKDQFDFIAVIDPSTFQAEFKT
                     LWSMCEFDNPLAALQHLLVSENMSSVWVKWPVKSLQPRKEAVKADYWYDYYSPHYPAQ
                     QAAYTATVIGLEGNVSGETIYMLQIAVDRSIVEYQLVKSLGVILLLDNTGNLVSETRP
                     HFDIIIKACISGVRDFVRQNPRMRSVEHQITSAI"
BASE COUNT         3279 a         3013 c         2939 g         3143 t
ORIGIN      
        1 taaccgtttt tcttttcagc ctaaaatacg tcgcccgtga tatacccgtt ttttccatta
       61 cctctttaac ttgtacgtcc ttattgatca attcattagc taattcagcc tttttatgag
      121 gctttcttcc aaatttgacg cccttaatca tagctgcctg ccgcccatcg ttagttcgct
      181 ccaagatcct ttcacgttcc gcctcggcaa cagctgctaa tatctggata accatttttc
      241 ccatagtgcc ttcagtacta agcccgttct caagaaagcg aatagcgatg ccttttttat
      301 agcaagtatc cacgatgcga atcatatctg cggtattacg tccaaggcga tccattttag
      361 tacagagaat aatgtcatct ttctccgctc tggcaaggag tcgctgtagc ccttcgcgct
      421 catcagttgc tcccgacatc atgtcagtaa acactcgatc atccctcact cccgctgtgt
      481 tgagttcagt aatctggctc gtcagtttct gatggctggt tgatactctt gcatacccta
      541 ataatgccat tttggtctca taaatcgata ttgatactgc gtagtatcat aaggcataaa
      601 tcacgatttt tgatacttat gtttagggcc gttttcattg gtctcaaaaa ttataatatc
      661 tgataccgcc aaaataatac ggatcaataa ataatgaaag gatgcgtgat ggctgctgat
      721 tttctgaccg acaaacagac acagaactac gggcgttatg ccgcagagcc aaacgagatc
      781 caactggcac ggtattttca tcttgatgag cgagacctta ctttcatcaa cctacgtagg
      841 ggcaggcata accggttggg gattgccctt caactcacaa ccgcccgctt tctgggaacc
      901 ttcttgcccg atctcatgca aataccgccc ggtgtccagt tttatgtcgc aaggcaactt
      961 aacatccgct atccagagat catctcccgc tatgctcaaa gggaaaatac acgttgggag
     1021 caccacgggc ttatcaggca gcactacagc tatcatgatt tcggtgattt cccgtggtcg
     1081 ttcagactga agcgattgct gtatactcgt gcatggctca gtaatgaacg ccccggactg
     1141 atgttcgatt ttgccaccgc gtggttgctt caaaataaag tgttgttgcc agccgcatcc
     1201 acccttacga gggtcattgg tgaaatccgt gagcgtgcga cccgccgctt atggcgaaaa
     1261 ttggccgcgc tgccaaaccg ttggcaggcc gcacaactgg ctgggttact tgaaatcccc
     1321 gaaggacagc gactctcagt gatggagcac ctaaaaagag gccctgtcac tatcagcggc
     1381 cccgcattca ctgaagcact tgaacgttac actcgcctgc gcagcctgga attttcctgc
     1441 ctgaatttca ctgggctgcc tgccatacag ctccgcaacc tggcacgcta tgccggaatg
     1501 gcatcggtga aatatatcag cagaatgcca gaagaacggc ggctggctat ccttaccgcg
     1561 ttcgtgaaag cgcaagaaat ctcggcgctg gacgaggccg ttgacgtgct ggatatgctg
     1621 atcctcaata ttaccaggga ggcgaagaaa accgggcaga aaaagcgact taggacgctc
     1681 aaggatcttg accgtgcggc gttgttgctg gcgcgggcat gtgcactttt acttgatgaa
     1741 gatactggtg atgatttgct gcgaaagacc atattcagca gtgtacctgt cgccagactg
     1801 gcagagtcag tggaaaaagt gaatgaactg gcccgccccc aggatacaaa ctttcaggat
     1861 gaaatggtgg agcagtacgg acgggtaagg cgttttttgc ccgcactact gcgcgatctg
     1921 cacttccggg cggccccaga cggtgaacat accctcgcgg ccatccatta cctggcagag
     1981 ctaaacggct cgaaaaaacg gatcctggac gatgcacctg agcatatcat ttcaggccct
     2041 tggaaacgcc tggtgtacga tgctgaaggt cggatacagc gagcgggtta ctcgttgtgt
     2101 ctgctggagc gacttcagga tgcgctacgc cgtcgtgaca tctggttgga aaacagcgat
     2161 cgttggggtg atccgcgcca aaaacttctt cagggggagg aatggcaggc gcagcgggtt
     2221 ccagtgtgcc gggcattggg tcaccccacc aatggcagta aagcatcggt gcaattggca
     2281 gcacggttgg atgaaacctg gaaaacggtg gcatcacgtt ttgaccgcaa taccgccgta
     2341 gacatctgca atgaaggtaa acatccttcg ctgaccatca gcagcctgga taaattggat
     2401 gagccaccgg cgctgatacg actgagcagc cgagtcagac aattgctccc acccgttgac
     2461 ctgacagaac tgttgcttga gattgacgcc agaacgggtt tcacccgtga attcagccat
     2521 gtcagcgagt caggtgcccg cgcgcaggat ctgcatataa gcctgtgtgc ggtgatgctg
     2581 gctgaggcct gcaatatcgg acatgagccg ctgatcaagc acaatatccc tgcactgacc
     2641 cgccatcgcc tgagctgggt gaaacagaac tacatccgag cagaaacgct ggtcagcgcc
     2701 aatgcgcggc tggttgattt tcagtcctcg ttagcgttgg caggatactg gggtggcggt
     2761 gaggtggcct ccgctgatgg tatgcgtttt gtcacaccag tgaaaacggt gaattctggc
     2821 cccaacagaa aatactttgg ttcagggcgc ggcattacct ggtacaactt tgtctctgac
     2881 caatattcgg gttttcatgg catagtgatc cctggaacgc tgcgggattc catttttgtg
     2941 ctggagggac tacttgagca acaaacggga ctaaacccgg ttgaaatcat gacggacaca
     3001 gcaggtacca gtgacattat ctttggcttg ttctggctac tgggatacca attttcacct
     3061 cgtctggcgg atgctggtga ggcggtgttc tggcgggctg acaaagcagc gaattacggg
     3121 gcgttggacg aactggctcg gggttgtgtt gacctttcta aaatcgaaag ccactgggat
     3181 gagatgatgc gggttgcagg ttcgctgaag ctgggcacaa ttcatgcgtc agaactcatt
     3241 cgatcgctat tgagaagtac ccgaccgtca gggcttgctc aggcaataat ggaggtcgga
     3301 agagttaaca agacactgta tcttctcaac tatattgatg atgaggacta tcgccgcagg
     3361 atcctaacac agcttaaccg gggcgaaggt cgccatgccg tggcaagagc catttgctac
     3421 ggtcagcgtg gtgagatcag aaaacgttac cgcgaggggc aggaagatca gttgggtgcg
     3481 ctgggtctgg tgacaaacgc agtggtgctg tggaatacgc tttatatgca ggaagccttg
     3541 tcgcacctgc gtagcactgg ggaagggcca gaagatgaac atatcgcgcg gctttcgcct
     3601 ctgatgcatg gtcatatcaa catgctggga cattatacgt tcacactacc ggaggatatt
     3661 atgaaggggg aattgaggcc gttaaatctc aatttaaaca atgaattacc tccttagcgt
     3721 ggttttttac atcattggcc ctcaaacccc ttatatgcgt tcttaacgtt tgctgcccac
     3781 tgttcaatgc ggagcatcat tgcatatgtt gctgcggcat caagatccag ttgttcagag
     3841 tgttcaggga gcagaatttt acaatattcc agtcatgagc cacagctccg agccgttcat
     3901 aaagtgtaga tagttgcgtt atggaatcat cagtgcggtt catgaatagc gccgacttcc
     3961 aataatatat tgagcaagtt tgatgttaag acataccatg ttgcttgata gttggcgcat
     4021 catacgggat tcgctacgaa ttaaatagga ttcaagtagt ccagaatatt cccctgacgt
     4081 tacaaccgtc aactgggcaa tgtgattatt taatttgctt gcccctgcca ggatatcatc
     4141 ctccgtcgcc tcgatataaa cgggggaggc agcgataaca taatcatcga aaggtcttgc
     4201 tgccatcaat gtctcaatag atttcgccgt cctttctttc ccgaatgtat ttgttaattc
     4261 ccgccaccat tgtcggtgac tgaacggtaa atcatgaaag gtggcttcat aagcgtccac
     4321 gaggtcacga ctattcaaaa aacccaaccc ggccgagatc acccacaact ccaggttctc
     4381 agttgtccgt aatatctctt ttgccgttga ccagtgatta ccggcataga gcgacagggc
     4441 cggtaccggt gaagcacttt gcgaccgttt aatcgttgct gcccagctgg acattgcctc
     4501 ttctggtgtc tgtccagacc ggatactcag ttctggcatg gagccgcact ggctgtgctt
     4561 acctttggta caggacgtta ttaggtgtgt aattcgcgac gccgccattt tgtagccctc
     4621 agccaaatgt gtgtaatcac tgtctaacta tcgtaacagc ccctcatccg ccataacgac
     4681 aaagaggaag ccctaaacgt aaacgactac cccattatac atttagtcat gcctttacta
     4741 ataactagat accaaagtaa acaccccaga ggtgacaccg tcacgttttt cggctttatt
     4801 tagttcattt tatgtactat gggatttaat tatgggtaaa tcccatagat ttacacctct
     4861 actcaaaaga gcctaataga aactaaatcc ttatatatca ataacttgaa ttcgccatca
     4921 cgcacccata gagagcaccc gtcttttgtc gggccgtagg gttgttggcc ccatagtaac
     4981 tgcatgctaa tctctttcat gcttagtcac tagtaaaggc tttactaatt gcgatgctac
     5041 gtgaatatta ttgacggagg agggggttgg ggcagtgaaa atctctcagc caaaacagtg
     5101 ggaaaaggat cgtgaaatga gcatgaatta tcagccggaa ctgaagttga gtacccgcac
     5161 cgaacaactc gccagtagtc gtgatgccgc tatgcaaaaa tttctggacg gtattatgct
     5221 gattgccgaa gcctcagcta tttgcgggtt ttcactattt aacagcaaaa ttatggcccc
     5281 taatgctttt ggccttcctg cgtccctggc cgcaagcatc gaggaagggc gacagcaaat
     5341 agaccgtaag acatggaaca atctttttga agaaacgggt attgaccgat tctggaatca
     5401 caaccagcga gcggagttca gtgagtctct ccggtccgct ccgccaattg cgtcgttaac
     5461 cgtaatccgt agcacattgc gccaggcagt ggccattcgg agcatcacgc ttgctcaagg
     5521 gtttgttgat ctgctatgcc agcttgatcg ccggtataaa actaatgccc agcaatttgt
     5581 gatgccaaaa aaactggtac tacgtggcat ttttcccgac tcgaacatga tgagatataa
     5641 cggcttttct caggataatc tcttctattt gaacgacttt gagaatatcg tttgcatctg
     5701 ctccaacgca gcgacacctc ctgtcggcag tggattgaat atgtacgacc ggcttgctgc
     5761 cttaaggaaa accgatttta ctggtgacat taccgacccg aagggctgga agtgccgatt
     5821 gttcgtaaat ggtaatgtcc atatttcagt tgagtgtgaa agcctgcata acacactgaa
     5881 tgacctgatt agcatttatt ttgccaacca gatcccggcc aaaggataag aatgatgact
     5941 gacaatgaaa atttgatgaa cggggatgaa cagacagcgt ccaatttaag tgaagagaca
     6001 acaaaaaagc gccctctcag ccccgaacta ctgaagaatt ctccttattc atcaatagct
     6061 aaggttaaat atttcaccga gattgaacaa atctgcctcg ataagggcat cagcaccgaa
     6121 aacctcgatc agtttttcaa agcccactgg ctgcgggata aaatgggagg gtcttttgcc
     6181 cgcgctcagg agatgctggc tgcctacaag caatacgtgg acgaggtgcc cgaggaagcg
     6241 cgtgcgattg aaatccctga ccagattaaa gaagcattta gtgattttac ggcgtttatc
     6301 atctggtact tccgcttaag ctatacggct atccagagtg atagcgtgaa aattgcgaag
     6361 gcagaaatta ctcagttaag acatcggaat gctgaaattc tggaagaact gtcacagtcg
     6421 aaagaacagg ttacagtact gaataacgaa aaagttaacc tgattaacca gctggaacaa
     6481 cagcgtgaac tcgccagtaa attgggagat tcacttcggg aagcagaaga aacgctggct
     6541 acaacccaaa gtgaattaca gtatgcgcaa aacgagattc agcttataca gcagacagta
     6601 gggacactga accaacaact gagtgagcgc aagcaggaac tggtctccca gcaggagtac
     6661 cagaagcagc tgaatgatga gaataaagcg cagcaggtag agctgacagc cctcaaaagc
     6721 cagaatgatc acttacaacg gaccgtgtct gatttaaaag tcagtgtcag tcagcttgaa
     6781 caggaccttt cctcagtaca gacccattcc tctgaattga gctcatcttt agccgaaaag
     6841 gacacgaccc tgacgctcgt gcggtcggag ctctcaacag ctaaagggga gaatgaccaa
     6901 ctacgagctg aagcacaacg cttagctgat gagagcctgg ttgccaaaaa agtgcaaaca
     6961 gatcagacag aagagcttca acagctcagg aatcagatga tttccatgga agcgacactc
     7021 aatgcggaaa agaccattgc cgagtcttta cgaggaacaa tcaagcagct taccgaagct
     7081 atgaccggcg cagttgccat caaacctaaa tcctctgggg catcaaaacc gcgaaacaaa
     7141 aagacgacat aactgctccc gccaggaatt tgcaatagac ttaaattcct ggcgtccaca
     7201 ggctctttat cccgttcgca ggagtttacg tccttccgac agcattaggc tgattttccc
     7261 cgaatcactg atctcaatag catcaaccag gctagtgatc ccgccaacag cttgctgttg
     7321 tgaaatagct cccagtttgt agtcatcgag tactgccgcc aaaaacgcct tcagtttaac
     7381 ctgctcactc atcgaaagta tgtacgccat ttttagcccc attttaaggt tgtgattcgg
     7441 gtgaattgta tcgtctagct tccgggaaat gagggagttc ctggtattca gtcttctccg
     7501 tagcatccca gtgccaccat ccataggccc catattcctg gttgtaatac gccttcttaa
     7561 gttcctttcg cgcaagatag gcaggtgtcg acatcccgaa aacttccatc gggaacggct
     7621 tatcacttcg tgtatccagt aaataaaaat ctgggaaaac atcgctcgca ctggcgtcat
     7681 accgcatagg cttaacatag tgccggtgtt cggcatcaag tttacgggac actatcgctt
     7741 cgtaggagga gtccagaggt atccagttat cactcaccag cataagcact atctgatgag
     7801 cccgtgcaga gatacctcta ctggtcaccg atacgggaga tgtaagagca aaaacaacaa
     7861 ctttgccccc atttttccag gcggcatatt ccagagggaa tcgccgcttc acgctatccc
     7921 agtgcccgtc tgagttaaag aaggtaaggg gcataccgcc gaagttacgc ataggtagaa
     7981 acttcagcgg cttctcatgc ttttctgcgt catagcgcgg cagtactgat agcagcagca
     8041 gtcgtttgtc gctcagttcc gccgacgaca gcagttgaac ctgctcactg gccactttgc
     8101 ttttagagtc cgccaccccg ataaaaagat ggtcgccaat acatgctcta cccgatctaa
     8161 tctgctttgc ggcttccaac atccggtacc gaaccagcga atcatttcgc ttcccggcca
     8221 tattcggata ccagacatta agaccggctt cagtccacaa cagactcaga agtccggaca
     8281 gggtcattga agcctggcca ccttcaggtc gctggatttg gggcagggaa ggaacctcag
     8341 acttttccgg cggatctttc tccgtcatgc ctattccaag gcggattgac atggtcccct
     8401 cattgttgat gcgtacaaca ttgctggcat aaccctttaa ccctgtatgc ctggcatcaa
     8461 ggtcgaaaaa cacacacgat ggatcatgct cagtgccggt atttgcagcc ttaatcagga
     8521 tgaacgtatc actgtttggt agtgagcgga cgtagagacg ctttgcgcct tttccccgac
     8581 atccgcagct gatcacagat ttctgcccgg acgtgtggtg tgctttcttc agggccgact
     8641 gccatccctt tacatactca tcctgtgtct gaaattcagc cgagtatcgt ttcgtgtttg
     8701 gttcgtcatc gtaagcaata aaaactgcaa acttctggcc catgggacct cctgaaattt
     8761 aaaaatacta atagcctagc catatcaata cttcttgcgc gttgtactct tccctctcac
     8821 ccaactcgga aaaaaatacg tctatgtcgc tggctacgac gccatgtttt gcaagctctt
     8881 gcagagctct ctgttttgaa atggtgaggt cttctgctga atcaaaatac gtcatcatta
     8941 cctcctgatg aaggagttga ctgaagcagg cctttctctc cctgcccagg tttttttctg
     9001 tgcaaaagtt acaatattag tgaaataaac cattacgtta acctgcaaaa tcacacagaa
     9061 gaattactat gatgccgctt caaattctcc cttgagacga gccgcaacgg cgatctcaat
     9121 acggcttcga agcaaactgg ccacggccgc aaatacgtcg acgatgaccg aattgccgaa
     9181 ctggcgatag gcctgggtat cagagactgg aatgcgaaaa gtaacttttc ccggttgctc
     9241 gaatcccata aggcgggcac attctcgcgg ggttaaacgg cgcgggcgcc ttgccatatt
     9301 tacaggatca ttaaagtcct tttcgcctat gctgtgatcc catccacggt ctagcaaaat
     9361 ttcagagcca tcacgatagt accgtgcgct gagtgttctg caaacaactg atttatcaag
     9421 tggatttacc agtccgaagc caaatccatt accttttgcc tggtgctttt tagcgtagtt
     9481 ataaaggtac tcccagagct ttggagaaag gatgtaacgg ctatcgacct cagcgtccag
     9541 cagctcccca agggttggcc gtcgtgaagg ataaagagcc gatacaccac ttaacgaaaa
     9601 gccatcatgg agcttcagat cgcgcctgaa tccaaccaac acgatacgtt cccggtgctg
     9661 aggcctgaag ttcttaccgt caattatttt ggggtccttt gatccgtgag catcgcagtc
     9721 ggcaacatcg tagccaagtt catcaagcgc tttcatgatg atgcggaacg tatttccctt
     9781 atcgtggctc ttcagatttt ttacgttctc aagaacgaaa atggcaggct gtttggctcg
     9841 gatgatccgg gcgacatcaa aaaaaagggt cccctgggtg tcgcattcaa agccgtgttt
     9901 tcggcccaaa gagttctttt tgctgacacc ggcaatactg aacggctggc atgggaaacc
     9961 ggcgagcaaa acatcatggt caggaatgga tgcagcgata gaagcatacg cctggtcttc
    10021 cgacacatcg ggaagattgc tgagtgtaat gtcccggata tctgagttga aatggtgctc
    10081 ggtgtcttcg cagtaccagt ttgcactgta tgtccggcgg gagaactgat tccattcgct
    10141 tgtaaacacg catttcccgc caatggcgtc aaaaccactc cggaggccac cgatcccagc
    10201 aaacaagtca ataaaacgga aaaatttctg gtcatagtct gctgggcgtg aggggagcaa
    10261 cgatcgaaga taatgatatt cttcctgggt tattcgacat tcattaactg aacctttgat
    10321 ctggcgaata agtgatgccc tcgaccagtc gctgccggtt gcagcattaa gttgcgcagc
    10381 caaatctttt acgtcataga tacttgcaac ctgaaccagt aatttatgga tagaggaatg
    10441 tgtctcatca tttagcaatg cagttattgc aggctcattt ctcgcgctgt acatccttac
    10501 tccgtgaaat gtgattaatt taggttctca gactaccacg cgcagaaaat tcaatttttc
    10561 tttaaagccc tttattttca tctcctgaaa taaatttttt atccacttaa attgcctccg
    10621 ttaacggaaa tttgcccggg ttctaattgg tacaataagc acttctaatc acacgactaa
    10681 tttgttacta caaaccatct aattcaggga gaatgtatga ctctgttgaa aaagttactt
    10741 ttgccgacat taattgtcct tttcccggcg gcagttctgg ctggtcagtc ttcatttgaa
    10801 gcaaaagtgg taaaaattat cgatggggac accattaccg cgctcgacgg tcagaataca
    10861 agcatcaaaa tccgtatgta cggtattgat gcccccgaaa gtaagcaggc ctttggacaa
    10921 aaggctaaac aagcgttgag ctccgcgatt gctgcacaga atattacggt aatagaccac
    10981 ggacctgata tttacggcag gatgttgggg actatttggc ttgatggtta tgacatcaac
    11041 gcttcaatgg tagacagtgg ttatgcatgg gtatatcggt ttgacggtaa tcccatcgtc
    11101 ccaaactatc ttaaattcga agcttcagct cagaaagccg ttaaaggtct ctgggtagat
    11161 cctaatcccg ttgctccttg ggagtggcgc cagcaaaacc agaaacctca aaagacgaaa
    11221 agcagggggt gagcgcgatg acaagccagc atcctttact gaaaattgca cttaatggcg
    11281 atggacttct ttcgtggcca aattcacccg gacagactat gcgctgccca acgtgtatgc
    11341 gagatgttat tacgggtccg gatggtgatt taatacatca aatggaagag ggcgctaacc
    11401 cctgcacgcc atctgttgat gttgtcattt caaaggcgat cattgaacta ctcagcacgg
    11461 gtgagcgcct ctatgtcaac ccagttaaga acgcgaatag aacactggcc ccatcgttta
    11521 tctttctgca taaagacaag caacttcgac cattcataaa tacggaatat caaccagcag
    11581 gtgccacctg gagaagtgat aaagggaaca ggcttggttt tttctatatg gatgcgcgag
    11641 cgtctaaaat taataaggac cagttcgatt ttattgctgt aatagaccca tctacttttc
    11701 aagcagaatt taagacctta tggtccatgt gtgaattcga taaccccctt gccgcgttac
    11761 agcacttatt ggtttcagag aatatgtctt cagtctgggt gaaatggcct gttaaaagcc
    11821 tacaaccgag gaaagaagca gtgaaggccg attactggta tgactattat tcacctcact
    11881 acccagcgca gcaggcagct tacacagcaa cagtgattgg actggaagga aatgtatccg
    11941 gggaaactat ttacatgctc cagatcgctg tagaccggtc gatagttgag taccagcttg
    12001 taaaatcgct aggggttata ttgctattgg ataatacggg aaatttggta tcggagacgc
    12061 gcccacactt tgatataatc atcaaagctt gcatctcggg tgtcagagac tttgtacgtc
    12121 aaaatcctcg aatgcgaagt gtggagcatc agattaccag tgcaatatga ggaaatggat
    12181 cagcgactaa atctgttgct cgccgtaaat acaagtgcgc accataaaat aaaaacactt
    12241 tataaatcaa tgactttgtc aaggctcatt agaaatgtct aattactggc tcgttagaaa
    12301 tgtccatatg tcaaaaaggc agctgcgaga atttctcccc gcagctgcct ctttagcata
    12361 acgtcattat gcgc
//
LOCUS       DAFNBJ010000140        12102 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000140 DAFNBJ010000000
VERSION     DAFNBJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.140"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..376
                     /locus_tag="L2X49_003763"
     CDS             <1..376
                     /locus_tag="L2X49_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBN4798783.1"
                     /translation="NFLNVSGKNNCPTMQLMLFNLFSPALKLNTGLAILSPGAFEVHS
                     DGIDVDNELFHYPINKAYTPYNIHTYKTEEVVNQRNIKVKNMTLDEINNTYCNNDYYN
                     QAIREEPIDLLDRSFSSSSWPF"
     gene            700..2088
                     /gene="narU"
                     /locus_tag="L2X49_003764"
     CDS             700..2088
                     /gene="narU"
                     /locus_tag="L2X49_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBN4798784.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2170..5910
                     /gene="narZ"
                     /locus_tag="L2X49_003765"
     CDS             2170..5910
                     /gene="narZ"
                     /locus_tag="L2X49_003765"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HBN4798785.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSSSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5907..7451
                     /gene="narH"
                     /locus_tag="L2X49_003766"
     CDS             5907..7451
                     /gene="narH"
                     /locus_tag="L2X49_003766"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBN4798786.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7451..8146
                     /gene="narW"
                     /locus_tag="L2X49_003767"
     CDS             7451..8146
                     /gene="narW"
                     /locus_tag="L2X49_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBN4798787.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8143..8823
                     /gene="narI"
                     /locus_tag="L2X49_003768"
     CDS             8143..8823
                     /gene="narI"
                     /locus_tag="L2X49_003768"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBN4798788.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8902..9795
                     /locus_tag="L2X49_003769"
     CDS             8902..9795
                     /locus_tag="L2X49_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBN4798789.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9891..10736)
                     /gene="nhoA"
                     /locus_tag="L2X49_003770"
     CDS             complement(9891..10736)
                     /gene="nhoA"
                     /locus_tag="L2X49_003770"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HBN4798790.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10909..11478
                     /locus_tag="L2X49_003771"
     CDS             10909..11478
                     /locus_tag="L2X49_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBN4798791.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11482..11709)
                     /gene="pptA"
                     /locus_tag="L2X49_003772"
     CDS             complement(11482..11709)
                     /gene="pptA"
                     /locus_tag="L2X49_003772"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HBN4798792.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11760..12102)
                     /locus_tag="L2X49_003773"
                     /pseudo
     CDS             complement(11760..12102)
                     /locus_tag="L2X49_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2885 a         3114 c         3124 g         2979 t
ORIGIN      
        1 aaatttttta aatgtttctg ggaagaacaa ttgccctact atgcagctca tgttatttaa
       61 tttgttttcc ccagcactta agcttaatac tggcctggca attctttcgc ctggtgcatt
      121 tgaagttcac tctgacggaa tagatgtgga taacgaattg tttcactatc ctattaataa
      181 agcatatacc ccatataata tacacactta caagacagag gaagttgtaa accagaggaa
      241 tataaaagtt aaaaacatga ccttagatga aataaacaat acttactgta ataacgatta
      301 ttacaatcag gcaataagag aggaaccgat agaccttctg gacagatcgt tttcctccag
      361 ttcatggcct ttttagtgaa taaatcatca ctaattctat aagtgtctgg tcttgttgat
      421 aatatagtta atatgtaacc ctttagattc acgatctgaa gggttgaaac atgacatacg
      481 ttcaaagatt aacccccgtt tgaaggttag cttgttagtt acatttagta acacatatct
      541 ggctacttaa tccccattct gcccccgcgg ttacgctttc ttatctattc tttcaatcag
      601 cacttcaccc aattcatcct taattctaat aactggctat tgcgccttac ctcctgtttt
      661 gtcttttttc attaccaatg tgtgcatgtg aggaacaata tggcactgca aaatgagaaa
      721 aatagtcgtt atcttttacg cgactggaaa ccagaaaatc ctgccttctg ggaaaataaa
      781 ggaaaacata ttgctcgaag aaacctctgg atatcagtca gttgtctact tcttgccttc
      841 tgtgtctgga tgctatttag cgcagttacc gttaatctca ataaaatcgg ttttaatttt
      901 actaccgatc aactcttttt attaaccgca ttaccctccg tttctggcgc attattgcgt
      961 gttccctact cctttatggt gcctatattc ggtggacgcc gatggacggt ttttagtact
     1021 gcaatcctga ttattccttg cgtctggctc ggaattgccg tgcaaaatcc gaatactcct
     1081 tttgggatat ttatcgttat cgctttgcta tgcggttttg caggtgcaaa ctttgcttcg
     1141 agcatgggca atatcagttt cttctttcca aaagccaaac aagggagcgc tcttgggatt
     1201 aatggcggat taggaaactt aggtgtaagt gtaatgcagc tggttgcacc gctggtcatt
     1261 tttgtacctg tatttgcctt tctcggcgtc aatggcgtac cgcaggccga cggttcggtg
     1321 atgtcgctgg cgaatgccgc atggatttgg gtaccgctac tggcgattgc cacgatcgct
     1381 gcctggtcag ggatgaatga tatcgccagt tcacgcgcct caattgccga ccagctccct
     1441 gtcttacaac gcctgcatct ctggctgctg agcctgcttt accttgccac cttcggttcg
     1501 tttatcggtt tttctgcggg ttttgccatg ctggcaaaaa cccagttccc ggatgtgaat
     1561 attctgcgcc tggcgttctt tggcccattt atcggtgcca tcgcgcggtc ggttggtggt
     1621 gctatttccg ataagttcgg cggcgtgcgg gtgacgttga tcaactttat ttttatggcg
     1681 attttcagtg ccctgctgtt ccttacctta ccgggcacag gctccggtaa tttcatcgcc
     1741 ttttacgccg tatttatggg gctgtttctg actgcgggtc tgggaagtgg ttctactttc
     1801 cagatgatcg ccgtcatctt tcgccagata accatttatc gggtaaagat gaaaggcggt
     1861 agtgatgagc aagctcagaa agaagccgtc accgaaacgg cggcggctct gggctttatc
     1921 tcagccattg gcgcagtggg cggctttttt attccgcagg cgtttggcat gtcgctcaat
     1981 atgaccggct ctccggtcgg cgcgatgaaa gtgtttttaa tcttctacat cgtttgtgtg
     2041 ctgctgacct ggctggttta tggtcggcgg aagttcagcc aaaaataaga gatcgatgcc
     2101 tgatgtacaa ccgtgtcgca tccgacaagg catcagatcc caattcattc ctggagcagg
     2161 agtcatgtca tgagtaaact tttggatcgc tttcgctact tcaaacaaaa gggcgaaacc
     2221 tttgccgatg gtcacggaca ggtgatgcat agcaaccgcg actgggagga cagctatcgc
     2281 cagcgttggc agttcgacaa aatcgtgcgt tccacccacg gtgttaactg tacaggctcc
     2341 tgtagctgga aaatctacgt taaaaatggt ctggtgacct gggaaatcca acagaccgac
     2401 tacccgcgca ctcgccctga cctgcccaat catgaacctc gcggctgccc gcgtggcgca
     2461 agttactcct ggtatcttta cagcgctaac cgcctgaaat acccgctcat tcgtaaacga
     2521 ctgattgaac tgtggcgcga agccctcaag caacacagcg atccggtact ggcgtgggca
     2581 tcgattatga acgatccgca aaagtgcctg agctacaaac aagtgcgtgg gcgcggcggg
     2641 tttatccgct ccaactggca ggaactaaac cagctgattg ccgccgctaa cgtctggacc
     2701 atcaaaacct acggcccgga tcgcgttgcc ggtttctcgc cgatcccggc gatgtcgatg
     2761 gtttcttacg ccgccggaac gcgttatctg tcgctgcttg gcggcacctg tttaagtttc
     2821 tacgactggt attgcgacct gccgcccgcc tcgccgatga cctggggcga gcaaaccgac
     2881 gtaccggaat ctgccgactg gtataactcc agctacatca tcgcctgggg gtctaacgta
     2941 ccgcagacac gtacgccgga cgcccacttc tttaccgaag tacgctacaa aggcactaaa
     3001 accatcgcca ttacccctga ctactctgaa gtggccaaat tgtgcgacca gtggctggca
     3061 ccgaaacaag gcactgatag cgccctggcg atggcaatgg gccatgtgat tttaaaagag
     3121 tttcatctcg ataatcccag cgactacttt atcaactact gccgccgcta cagcgacatg
     3181 ccgatgctgg taatgctgga gcctcgcgac gatggtagct acgttcccgg gcgcatgatc
     3241 cgcgcatctg acctggtgga tggactgggc gaaagcaaca atccgcagtg gaaaaccgta
     3301 gcagttaata ccgcaggtga attggtagtg ccgaacggtt cgattggttt ccgctgggga
     3361 gaaaaaggca aatggaatct ggaatccatt gccgccggta cggaaaccga attgtcgtta
     3421 accctgctcg gtcaacatga cgctgttgca ggcgtggcct tcccctactt tggcggcatt
     3481 gaaaatccgc attttcgcag cgtaaaacac aatccggtgc tggtgcgcca attgcccgtt
     3541 aaaaacctga cgttagtcga tggcaacacc tgtccggtgg tcagcgttta tgatttggta
     3601 ctggcgaatt acggcctcga tcgcgggctg gaagatgaaa acagtgcgaa agattacgct
     3661 gaaatcaaac cgtacacccc agcctggggt gagcaaatta ccggcgtgcc gcgccagtat
     3721 attgaaacca tcgcccgtga atttgccgat accgcccata aaacgcatgg gcgctcgatg
     3781 attatcctcg gcgcaggtgt taaccactgg tatcacatgg acatgaacta ccgcgggatg
     3841 atcaatatgc tgatcttctg cggttgtgtc gggcaaagcg gtggcggctg ggcacactat
     3901 gtcggccagg aaaaactgcg cccacaaacc ggctggttgc cactggcctt tgcgctcgac
     3961 tggaaccgac caccgcgcca aatgaacagc acctcgtttt tctacaatca ttccagccaa
     4021 tggcgctatg aaaaagtctc tgctcaggag ttactttcac cgctcgccga tgccagtaag
     4081 tacagcggtc atctgattga tttcaacgtt cgcgccgaac gtatgggctg gctaccttct
     4141 gcgccgcagc tggggcgtaa cccgctcggg attaaagctg aagccgacaa ggccggatta
     4201 tcccccacag aatttaccgc ccaggcgctg aaatcgggcg atttacgtat ggcctgcgaa
     4261 caaccagata gcagcagcaa tcatccgcgt aatttgtttg tctggcgctc taacctgctt
     4321 ggctcctccg gcaaaggcca cgagtatatg cagaagtatc tgctggggac cgaaagcggg
     4381 attcagggcg aggaactcgg tgccagcgac gggatcaaac cggaagaagt cgagtggcaa
     4441 actgcagcga ttgaaggcaa gctcgacctg ctggtgacgc tcgacttccg catgtccagt
     4501 acctgcctgt tctccgatat cgttctgccc accgccacct ggtacgaaaa agacgatatg
     4561 aacacctcgg atatgcatcc gtttattcat ccgctttctg cggcggtcga tccggcgtgg
     4621 gaatcacgca gcgactggga aatctacaaa ggtatcgcca aagcattttc gcaagtgtgc
     4681 gtgggtcatc ttggcaaaga aaccgacgtg gtattacaac ccctactgca tgactctccg
     4741 gcagagctct cacagccgtg tgaagtgctc gactggcgca aaggcgaatg cgatctgatt
     4801 ccaggcaaaa ccgcgccgaa tattgtggcg gtggagcgcg actaccctgc tacgtatgaa
     4861 cgctttacct cgctcgggcc attgatggac aaacttggca atggcggtaa agggatttcg
     4921 tggaatacgc aggatgaaat cgatttcctc ggcaaactca attacaccaa gcgtgatggc
     4981 ccggcacagg ggcgtccgct gattgacacc gccattgacg cttcagaagt gattctggca
     5041 ctggcaccgg aaaccaacgg tcatgttgca gtcaaagcgt ggcaggcgct gggcgagatc
     5101 accgggcgcg aacataccca tctggcgctg cacaaagagg acgagaagat tcgctttcgc
     5161 gatattcagg cgcagccgcg taaaattatc tccagcccca cctggtccgg tctggaaagc
     5221 gatcacgtat cctataacgc gggatacacc aacgttcatg agttaattcc gtggcgcacg
     5281 ctgtcgggac gccagcagct ctatcaggat catccgtgga tgcgtgcttt tggtgaaagc
     5341 ctggtggctt atcgcccgcc tatcgacacc cgtagcgtca gtgagatgcg ccagataccg
     5401 ccaaacggct tcccggaaaa agcacttaac ttcctgacgc cgcaccagaa atggggcatt
     5461 cactcaacct acagtgaaaa cctgctaatg ctgacgctct ctcgcggtgg accgattgtc
     5521 tggatcagcg aaacagatgc ccgagaactg accattgtcg ataacgactg ggtggaagta
     5581 ttcaacgcca acggcgcgct gactgcccgc gcggtggtca gccaacgtgt accgccgggc
     5641 atgaccatga tgtatcacgc ccaggaacgc attatgaata ttcctggttc ggaagtaact
     5701 ggcatgcgcg gcggcattca taactcggtc acccgcgttt gcccgaaacc gacgcatatg
     5761 attggcggtt acgcgcagct ggcctggggc tttaactact acggcaccgt cggctcgaac
     5821 cgcgacgaat tcatcatgat ccgcaaaatg aaaaacgtta actggctgga tgatgaaggt
     5881 cgcgatcagg tacaggaggc gaaaaaatga aaatacgttc gcaagtcggc atggtgctta
     5941 acctcgataa atgtatcggc tgccatacct gttcggtgac ctgtaaaaac gtctggaccg
     6001 gacgcgaagg catggagtac gcatggttta acaacgtcga aaccaaaccg ggcattggtt
     6061 atcctaaaaa ctgggaagat caggaagagt ggcaaggcgg ctgggtccgc gatgtgaatg
     6121 gcaagatccg tccgcgtctg ggcagcaaga tgggcgtaat aaccaaaatc ttcgccaacc
     6181 cggtggtgcc gcagattgat gattactacg aacctttcac cttcgactac gaacatttgc
     6241 atagcgcacc ggaaggcaaa catatcccta ctgctcgccc gcgttcactg attgacggca
     6301 aacggatgga caaagtgatc tggggcccaa actgggaaga actgctgggc ggcgagtttg
     6361 aaaaacgtgc ccgcgaccgc aacttcgagg ccatgcaaaa ggagatgtac gggcagtttg
     6421 aaaacacctt catgatgtac ctgccgcgcc tgtgcgaaca ctgcctcaat cccagttgcg
     6481 tggcgacctg cccaagcggc gctatctaca aacgcgaaga agacggcatt gtgctgattg
     6541 atcaggataa atgccgtggc tggcgtttgt gcattagcgg ttgtccgtac aaaaaaatct
     6601 acttcaactg gaaaagcggc aagtcagaaa aatgtatctt ctgttatccg cgaattgagt
     6661 caggccaacc caccgtgtgc tcagaaacct gcgtgggtcg catccggtat ctgggcgtgc
     6721 tgctttacga cgccgaccgc attgaggaag cggcgagcac cgagcgcgaa gttgacctct
     6781 atgaacgtca gtgcgaagtg ttcctcgatc cacacgatcc ctcagtgatc gaggaagccc
     6841 tgaaacaagg tattccacaa aacgtgattg aagctgccca gcgttcgcct gtctacaaaa
     6901 tggcgatgga ctggaaactg gcgctaccgc tgcaccctga atatcgcacc ctgccaatgg
     6961 tctggtacgt tcctccgctg tcgccgattc agtcctacgc agatgcgggc ggtttgccga
     7021 aaagcgaagg cgtgctgccc gccatcgaaa gcctgcgtat tccggtgcaa tatctcgcca
     7081 atatgttgag tgccggcgat accggtccgg tactgcgggc gctgaaacgg atgatggcga
     7141 tgcgccacta tatgcgttca caaaccgtgg aaggcgttac tgatactcgt gccatcgacg
     7201 aagtaggcct gagcgtcgcc caggtcgaag aaatgtatcg ctatctcgcc attgccaact
     7261 atgaagatcg ttttgttatc ccgaccagcc atcgggaaat ggcgggcgat gccttcgcag
     7321 aacgcaacgg ctgcggtttt acctttggcg acggttgtca cggctcggac agcaaattca
     7381 acctgttcaa cagtagccgt atcgatgcca tcaacatcac cgaagtgcgc gacaaagcgg
     7441 agggcgaata atgcagatcc tcaaagtgat cggcctgttg atggagtatc cggatgagct
     7501 gttgtgggaa tgcaaggagg acgcgctggc gttgatccgc cgcgacgcgc cgatgcttac
     7561 ggatttcacc cacaacctgc ttaacgcgcc gctgctggat aaacaggccg aatggtgcga
     7621 agtgtttgac cgcgggcgca ccacgtcgct gctgctgttc gaacatgttc atgccgagtc
     7681 ccgcgatcgc ggccaggcaa tggtggacct gctggcggag tatgaaaagg tcggcctgca
     7741 gctggattgt cgggaactgc ccgattatct accgctgtat ctggagtatt taagcgtact
     7801 gcctgacgat caggcgaaag aaggattgct caatgttgcg ccgatcctcg ccctgcttgg
     7861 cggtcgctta aaacaacgcg aggcaccgtg gtacgcgttg tttgatgctc tgctgcaact
     7921 ggcgggaagc tccctttcaa gtgacagtgt cacaaaacaa gtaaacagcg aagagcgcga
     7981 tgacacccgc caggcgcttg atgcagtgtg ggaagaggaa caggtgaagt ttattgaaga
     8041 taacgccacg gcgtgtgaca gctcgccgct taatcaatat cagcgacgct ttagccagga
     8101 tgtcgcgccg caatatgtcg acatcagtgc gggaggtggg aaatgattca gtatctgaac
     8161 gtcttttttt acgatatcta cccgtacatt tgtgcgacgg tgtttttcct cggcagctgg
     8221 ctgcgctacg actacgggca gtacacctgg cgcgcctcct caagtcaaat gctcgataaa
     8281 cgcgggatgg tgatatggtc gaatctgttc catatcggca ttttggggat tttcttcggg
     8341 cacttgttcg gcatgttaac gccgcactgg atgtacgcgt ggtttttacc agtggcagcg
     8401 aaacaactca tggcgatggt gctcggcggt atttgcggtg tgttgacgtt gattggcggc
     8461 gcaggactgc tgtggcgcag gctgacaaat cagcgcgtgc gtgccacttc cactacgccg
     8521 gatatcatca tcatgagcat tttgctgatc cagtgtctgt tggggctaag taccataccg
     8581 ttttcagcac agtatcctga tggtagcgaa atgatgaagc tggtgggttg ggcgcaaagc
     8641 attgtcactt tccgtggtgg atcgtcagag atgctcaacg gcgtagcgtt cgtcttccgc
     8701 ctgcatctgg tgttgggaat gaccatcttc ctgctcttcc cgttcacccg actggtgcat
     8761 gtgtggagcg cgccgtttga gtactttact cgtcgatatc aaattgtgcg ttcgcggcga
     8821 taatttcacc ctggccggat agcccttcgc tctccggcca cttgcaggct atgatagttc
     8881 tccagtcaca ggagaacgtt aatgaaaccg caggtgtacc acgtcgatgc ttttacctca
     8941 caaccgtttc gcggcaattc tgccggtgtt gttttccccg ccgataatct tagcgaagcg
     9001 caaatgcagc ttatcgcccg cgagttaggc cattcggaaa ctgcttttct gctgcacagc
     9061 gacgacagcg atgtgcgcat ccgctacttt acgccaacgg ttgaagtgcc gatttgcggt
     9121 cacgcgacag tagctgcgca ctatgtacgt gcgaaggtgt taggtttagg aaattgcacg
     9181 atctggcaaa catcgctggc aggaaaacat cgcgtgacta tcgaaaagca caacgatgat
     9241 tatcgtattt cgctggaaca aggtacgccg ggctttgaac tgccgctgga gggtgaagta
     9301 cgcgcggcga ttatcaacgc attgcatctt actgaagacg atattctgcc gggtttgccg
     9361 atccaggtcg cgacgaccgg gcattctaaa gtgatgatcc cattgaagcc ggaagtggat
     9421 atcgacgctc tttcgcctga ccttaatgcg ctgaccgcta tcagtaaaca gattggctgc
     9481 aatggtttct tcccgttcca gatccgccca ggcaaaaacg aaaccgatgg tcgcatgttc
     9541 tcgcctgcga ttggtattgt ggaagatccg gtgactggca atgccaatgg cccgatgggt
     9601 gcatggttgg tacatcacaa cgtattgccc cacgatggca aggtgctgcg tgttaaaggc
     9661 catcaggggc gcgcattggg gcgtgacggc gtgattgaag tgacggtgac aattcgtgat
     9721 aaccaaccgg aaaaagtcac catttctggc gcggcggtga ttttgtttca tgccgaatgg
     9781 gcaattaaac tctgaaaccg ttgcctgatg cgcttcgctt atcaggtcta caaccgagct
     9841 aatgtatgcc ggataaggcg tttacgccgc atccggcaac ctgacataaa ttattttccc
     9901 gcctccgggt gcgtatcaaa agccgccatc accagcgcta actcatccac ggtaaagcca
     9961 tgtttcgcat catccacgcc cagaccaaac tgttcttgca tcacagcata taatgacgcc
    10021 acatccggta gatttcgctg ctccaccgcg tgcccatttt cataatgggt aaaatgaaaa
    10081 ttggtcagtg tcagcttgcc gccgtccggc aaatggcggc acatcagcaa atgatggcga
    10141 aaatgcgact gcggccagtg cgccgaccag aaattgccca tcacataatc gctttgttgc
    10201 tgctcgcaga gatcaaaacg gtacatcgac tgccagtgct gatgatgatt aaactgcaac
    10261 acccaatcat caccctcctg caacaaccga taatctccgt gtggcgtggt ctgcacgaga
    10321 tcggaaacta aacgaatcgg cgcggttagc gtctgcccac cgaaaccgac atcagcaatc
    10381 catttttcct cttccagttc caccaacagc aaacgatggg tgcgcggcgg taatgctggc
    10441 ggatttgata acactacgcg ccctaacaag ctgcgaacgt taaactccag ctcgcgtaac
    10501 acccgctcaa acacgccatt ctgctcaaaa cagtaaccgc cacgacgggc tatcaccagt
    10561 ttctcttcca gcgattgatc atcaagctgt atttccctcg gcagcaaaac gtcgaggttt
    10621 tcaaacggaa tagtgcaatt gtgtttcagg tgcaatgcac gaagcgtatc aatattgacc
    10681 gcagcagctc ccgaccagtt aatacgggca aaatagtgat tcagaatggg cgtcatcagg
    10741 cgtttccctt agtggttttc tcaacttata acctggaata gacgccgcac cggctatttt
    10801 gtgcgatcgc ggacatgcat cacgtttgca aatcgccaat cccgttaatt tcgttactta
    10861 ttgcgttacg gactacgctt aaaaaaacct tcttcgcgag gcgataccat gagccgattc
    10921 atccccatag aattacacca tgccagccgt ctgttaaatc atggcccaac tgtcatgatc
    10981 accagtttcg atgagcaatc ccagcggcgt aacattatgg cagcagcctg gtcaatgccg
    11041 gtggagtttg aaccgccacg cgtggcgatt gtggtagata aatcgacatg gaccagggag
    11101 ttgattgaac gtaacggtaa atttggcatc gttatcccgg gcgttgcagc aactaactgg
    11161 acgtgggcag tgggaagcgt gtcgggtcgt gaagaagata aatttaattg ctatggcatt
    11221 ccggtggtga aaggtccggt gcttggtttg ccacttgttg aagagaaatg cctggcgtgg
    11281 atggagtgcc gcctgctacc tgcgacttct gcgcaacaaa aatacgacac gctgtttggc
    11341 gaggtggtat cagcagcggc agacgcacgg gtatttgtcg aaggccgctg gcagtttgat
    11401 gatgataagc tcaatacgtt gcatcattta ggtgctggca cgtttgttac cagcggcaag
    11461 cgagttacgg cgggttaagc attacatgct gtaaccaggt ttctttatca aagcctccat
    11521 ttggggcgcg atttcggcat cccagatagc ttgccaagat tctggttgaa tctgctgtag
    11581 agcaatgctt atcgaactgt ctttactgtt cagatggcga ataataacgt cggtaatatc
    11641 tgcagcaagt gctgcttttt gttgttcgtc cagttcacgc ggaaaacatt taatgtcgat
    11701 gtgcggcatg attgtgctcc ttttgaaaga tgtcagtaaa attaacacac attgtgaatt
    11761 ttcagtcctt tccattcaaa cgtgaaatca ataagttcat cagggacatc gcaaacaata
    11821 tgaatacgga tttcttctct gccgtgactc ttttcacaaa ttgcgtaact gtcatgctct
    11881 ggattattta attctttcag cggaaatttt tcctcaaagg ctttatttag ccacccctgg
    11941 tttcctttca ccgcgaataa ataatcacct ccctgttttt gtatcttctc tgctatatct
    12001 ttctggcaac ccatcgcatc agttgtgatg atttttcgtt taatatccag catgttaaga
    12061 agttctggga tagctgtaat ctcattagat ttatcatccg tc
//
LOCUS       DAFNBJ010000141        12086 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000141 DAFNBJ010000000
VERSION     DAFNBJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.141"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..392
                     /locus_tag="L2X49_003774"
                     /pseudo
     CDS             1..392
                     /locus_tag="L2X49_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            331..681
                     /gene="tnpB"
                     /locus_tag="L2X49_003775"
     CDS             331..681
                     /gene="tnpB"
                     /locus_tag="L2X49_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBN4798793.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEDQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            712..2325
                     /locus_tag="L2X49_003776"
     CDS             712..2325
                     /locus_tag="L2X49_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HBN4798794.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(2363..2559)
                     /locus_tag="L2X49_003777"
                     /pseudo
     CDS             complement(2363..2559)
                     /locus_tag="L2X49_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305349.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            2804..3190
                     /locus_tag="L2X49_003778"
                     /pseudo
     CDS             2804..3190
                     /locus_tag="L2X49_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3210..3407
                     /locus_tag="L2X49_003779"
     CDS             3210..3407
                     /locus_tag="L2X49_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HBN4798795.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAWEDIRTLRQLQLPDQNR"
     gene            3492..4337
                     /locus_tag="L2X49_003780"
     CDS             3492..4337
                     /locus_tag="L2X49_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBN4798796.1"
                     /translation="MSAITVSRPEVVNGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISSMTESIGGKTSLDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANIWSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRGDRWGLHLITGQQADRLADLERMLYLFSGKPIPDNR
                     ENIIIRLDDHIRSVQGKACYEDEMFNIRYFKKGSAHITFRKPELVDRLNDIIARHYSE
                     MLPSL"
     gene            4572..4700
                     /locus_tag="L2X49_003781"
                     /pseudo
     CDS             4572..4700
                     /locus_tag="L2X49_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            5130..6314
                     /locus_tag="L2X49_003782"
     CDS             5130..6314
                     /locus_tag="L2X49_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HBN4798797.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYDSVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            6425..7348
                     /locus_tag="L2X49_003783"
     CDS             6425..7348
                     /locus_tag="L2X49_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBN4798798.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(7352..8170)
                     /gene="nlpA"
                     /locus_tag="L2X49_003784"
     CDS             complement(7352..8170)
                     /gene="nlpA"
                     /locus_tag="L2X49_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HBN4798799.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            8392..8685
                     /locus_tag="L2X49_003785"
     CDS             8392..8685
                     /locus_tag="L2X49_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798800.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(8726..9916)
                     /gene="nepI"
                     /locus_tag="L2X49_003786"
     CDS             complement(8726..9916)
                     /gene="nepI"
                     /locus_tag="L2X49_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBN4798801.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(10127..10579)
                     /locus_tag="L2X49_003787"
     CDS             complement(10127..10579)
                     /locus_tag="L2X49_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBN4798802.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVIPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(10632..11966)
                     /gene="adeQ"
                     /locus_tag="L2X49_003788"
                     /gene_synonym="yicO"
     CDS             complement(10632..11966)
                     /gene="adeQ"
                     /locus_tag="L2X49_003788"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBN4798803.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
BASE COUNT         3235 a         3032 c         2859 g         2960 t
ORIGIN      
        1 ttgtgttgca cagattgcac gtgaaaatgg cgtcaatgat aatgttattt tcaaatggct
       61 caggctctgg cagaacgaag ggcgtgtttc gcggcgtctt ccggtaacga cctcttctga
      121 cactggcgtt gaattattac ctgtagaaat aacgccggat gagcagaaag aacctgtggc
      181 ggccattgcg ccgtctttat ccacttccac tcagaccaga gtcagtgcca gttcctgcaa
      241 ggtggaattc cgtcacggta acatgacgct ggaaaatcca tcgccagagc tgctcacagt
      301 gttgatccgt gaactgaccg ggaggggaag atgatctcac tcccatcagg tacccgtatc
      361 tggctcgttg ccggcgttac cgatatgcgt aaatccttca acggactggg agaacaggta
      421 caacatgtgc tgaatgataa tcccttctcc ggtcacctgt ttatcttccg tggccgacgg
      481 ggtgacaccg tcaaaattct ttgggctgat gctgatggtc tgtgcctgtt caccaaacgc
      541 ctggaggaag accagtttat ctggcctgcg gtacgtgacg gcaaggtatc cattacccgc
      601 tcgcaactgg caatgctcct cgataagctg gactggcgtc agccaaaaac atccagccgt
      661 aactcactga caatgttgta aaaaactcct gaccgcatta taaaaacggt catgagtcag
      721 aaatacctca ttcgcatcgc agagctggaa agattgctct ctgagcaggc tgaagccctc
      781 cgtcagaaag accagcaact gagtctggtt gaagagacgg aagccttcct gcgctctgca
      841 ctgacacgtg ccgaagaaaa gatcgaagaa gatgaacggg aaatagaaca tctgcgggct
      901 cagatagaaa aactgcgccg gatgctgttc ggtacccgtt ctgaaaaact gcgtcgtgaa
      961 gttgaactgg ctgaggctct gctgaaacaa cgtgaacagg acagcgatcg ttacagtggg
     1021 cgggaagacg atcctcaggt tccccgccag ttgcgacagt cgcgccatcg tcgtccgtta
     1081 ccggcacacc ttccccgtga aatacaccgc ctggagccag aagaaagctg ttgcccggag
     1141 tgtggcggtg agctggatta tctgggggaa gtcagcgctg aacagctgga actggtgagc
     1201 agtgccctga aagtgatccg cacagaacgg gtaaaaaaag cctgtacaaa atgtgactgt
     1261 attgttgaag caccggcgcc gtcccgcccg atagagcgtg gtatcgcggg ccccggatta
     1321 cttgcccgcg tgttaacggg aaaatactgc gaacatctgc cactgtatcg tcagagtgaa
     1381 atctttgccc gccagggtgt cgaactgagc cgggccttac tctccaactg ggttgacgcg
     1441 tgctgccagt taatgacacc ggtgaatgat gccctgtacc gttatgtaat gaacacccgc
     1501 aaggttcaca ctgatgacac accggtaaag gtactggcac cgggtcagaa aaaggcgaaa
     1561 acagggcgta tctggacgta tgtccgggat gatcgcaatg tgggttcgtc atctcctcca
     1621 gcggtctggt tcgcgtactc gccgaaccgg caggggaaac acccggagca acacctccgc
     1681 cccttccggg gtatcctgca ggcggatgcg ttcacaggtt acgacaggtt gttcagtgca
     1741 gaacgtgaag gtggtgcact aacagaagtt gcgtgctggg cccatgcccg gcgaaaaatc
     1801 cacgatgtat acatcagcag caaaagtgcg acggcagaag aagccctgaa gcgaatcagt
     1861 gaactgtacg ccatcgagga tgaaatacgg ggattaccgg agtcagagcg tcttgccgtc
     1921 aggcagcagc gaagcaaagt gttactgacg tcgctgcatg aatggatggt ggagaagaat
     1981 ggtacgctgt cgaaaaaatc cagactgggc gaagcgttca gctatgtact gaatcagtgg
     2041 gatgccctct gttattacag tgatgacggt ctggcggagg cggataataa tgctgcggaa
     2101 agagcgcttc gtgcagtctg tctcggaaag aaaaacttta tgttctttgg cagcgatcac
     2161 ggcggcgagc gtggagcact gttgtacggg ctgatcggca cctgccgtct gaacggtatc
     2221 gatccggaag cgtatctgcg ccatatcctg agcgtactgc cggaatggcc ttccaaccga
     2281 gttgatgaac tcctgccatg gaacgtagta ctcaccaata aataagcgtc aatacggtgc
     2341 tccgttgacg cttaccaaca ggtcaaaaac cgctgtagcc agagttacgc tggcctgatg
     2401 ctttagtacc gggcttcgtc agataatcca gacgtgccaa taagcgctga tactgctcag
     2461 ggaaatcagg atcgttaata tcctggatgt cacgtccatt agcagggaaa tgaacaatgc
     2521 agcccctgga ttaactatgc agaaatcgtc ctgaggcact gataaatacg gagaggactc
     2581 tcacgtatgg ttgattgaca ccacagtgcg aattcggcga atgcgagatc acagtacgat
     2641 tccgcaccac actaccaatc aataccccgg gttttattca ggtaagcagg actgcggata
     2701 tccggtgtcg cgccgttgtg tcacgaacgg ggcaggtgcg aaacaccgga taaaatgcag
     2761 gttgcgatac ctctgaacgc cctgcgcaga gcggcgattt tggattaaca aggtctctga
     2821 aaacggttat tcactccgcg tggtcgataa atccgaccaa cacgcaacca gtacaccacc
     2881 accgttaaca acccttacct gcatacgttg cagtaccgca catatcacgg aaacggacaa
     2941 cgcctggctg tactcgctgt cacaccagac taatgacgac ggcgaaacag aatggattca
     3001 ttttacaggc agcggctatc tgttacgtac cgatgcatgg tcgtacccgg ttctgcggct
     3061 taaacgcctg ggcctgtcaa gaacgttccg tcgtctggtc gtcactctca cccggcgtta
     3121 tggcgtcagt ctcattcatc tggacgccgg cgcagaatgt ctgccggggc tccccacttt
     3181 cgactggtaa tcaggacaaa agacactgga tgaaattatt aaccacagaa acggcgctgg
     3241 atattctgat tgcgtggctg caggacaata ttgactgcga atcagggatt atcttcgaca
     3301 acgatgagga taaaacggat tcggcagcac tgctgccctg tattgaacag gcctgggaag
     3361 atatccgtac cctgcgccag ttgcagcttc cagaccagaa ccggtgagtc tcattcacca
     3421 tacttcattc cactgacgcc agcctgaaca gggctggcgt tttcatttat ctgcgaaaag
     3481 gaatatcgat tatgtctgca atcacagtct cccgtccgga agtggtcaac gggcatacgg
     3541 acgttatctg ctccacctca gtcagccaca ttctggctgt gcgaaagact acactgctgc
     3601 aaatcgacac acttatccgg caactggctg aaatctcatc aatgacagaa agtattggag
     3661 gtaaaacttc tctggactgg gccatgaaac aggattttcg ctgtggctgc tggctcatgg
     3721 agaaaccgga aaccgcaatg aaagccatca cccgcaatct tgaccgcgaa atctggcgtg
     3781 acctgatgca acgttccggg atgctgtcat taatggatgc gcaggcccgt gatacatggt
     3841 accgctcact ggagtacgat aactttccgg aaatcagtga agcgaacatt tggagcacat
     3901 ttgagcaact gcaccagaat aaggatgaag tgtttgagcg aggagtgatc aacgtcttca
     3961 gagggctgag ctggaattac aaaaccaact ccccctgtaa atttggcagt aaaattatcg
     4021 tcaacaatct ggtgaggggg gaccggtggg gattgcatct tatcaccggg caacaggcag
     4081 atcgactcgc cgacctggaa agaatgctgt atctgttcag tggcaaacca atccctgaca
     4141 accgggaaaa tatcatcatt cggctggatg atcacatccg ctccgttcag ggcaaagcgt
     4201 gctatgaaga tgagatgttc aacatcagat actttaagaa gggctccgca cacatcacgt
     4261 tcaggaaacc agaactggtt gatcggctaa atgatattat tgccaggcac tattcggaaa
     4321 tgttaccttc attataaagc tctaagaatg aattcaatta tgtattttag cttcattccc
     4381 tcacgcaaat ttatttgggg gttcttatgg gggttattca tttatccaac acagattaat
     4441 catttaaaat caatatatta atactcatat tcgactcctg tgatcttccg ccaaaatgcc
     4501 tctcctgacg ttctccaacc ccttgtaata ctgtcaatcc agatattttc tactgctcaa
     4561 tattcaatcg gcttcaggaa tacctggtct aaggttttgt gatggacgat gagcggctga
     4621 agaatccgcc tgtcggttct tctgtcctcg aacgcatccg cgatatgcgc gccagcatgt
     4681 gccgtgttta tttgcaggtg atttgccccc tgctggctct cagccaactg tatcgccatt
     4741 aaggtgaaga tgttgtttta ctcgccccag aacacccttt tttatacgac tattctattc
     4801 ctttaacagc tggcgaaaaa aactgaccta taaacttcac caataatcag actgccatca
     4861 agattaattt gttcatttaa tatatcaaat caacttgttt tatttttact atcaaaaata
     4921 gctcattgcc ttactataat taatatataa attcggtgat taattcttaa catgcttttt
     4981 ctaataatct caattgctgg ccctataata ttgcgctagc attgttttct ggttgtatca
     5041 gcgatactca aaaattctta atacaatact cattcgactg gtacttattt gtaactcagt
     5101 tatattttgt cgcccggtga ttcagaagaa tgcaaaaaac ggctaccact ccatcaaaaa
     5161 tacttgacct cactgccgcg gcatttttac ttgtcgcctt tctgacgggt attgcgggcg
     5221 ctcttcagac tcctacccta agtatattcc tcgcagatga actgaaagcc cgtcctataa
     5281 tggtaggttt tttcttcacc ggtagcgcta ttatgggaat tctggtcagt caatttctgg
     5341 caaggcactc cgataaacaa ggcgaccgta aattactgat tctgctatgt tgcttatttg
     5401 gagtgctggc ctgcacgctt tttgcgtgga atcgcaacta cttcattctc ctctcaacgg
     5461 gcgtacttct gagtagtttt gcttccaccg caaacccgca aatgttcgcc ctcgcccgtg
     5521 aacacgccga cagaacaggc cgtgagacgg ttatgttcag tacattttta cgtgctcaga
     5581 tctcgcttgc ctgggttatc gggccaccgc ttgcttatga actggcaatg ggatttagtt
     5641 ttaaagtgat gtatctcacc gctgccatcg catttgttgt ttgcggactg atagtctggt
     5701 tgtttttgcc atcaatacaa agaaatattc ctgtcgttac ccaacccgta gaaattttac
     5761 cctccaccca caggaagcgg gatacgcggc tactttttgt ggtctgttca atgatgtggg
     5821 cggcgaataa tctctacatg ataaatatgc cgctatttat tattgatgaa ctgcatctaa
     5881 ccgataaact ggctggagaa atgattggta tcgctgccgg tctggaaatt ccgatgatgt
     5941 taatcgcagg ctattacatg aaacgtattg gcaagcggct attaatgctc attgctatcg
     6001 tgagtggaat gtgtttttac gacagcgtac tcatggcgac gactccggcg gttgagctgg
     6061 aattgcaaat tcttaatgcc atcttccttg gtattctctg tggtatcggc atgctttatt
     6121 ttcaggactt gatgcctgaa aaaataggct ctgcgacaac gttatatgca aatacttcac
     6181 gcgtcggctg gattatcgcc ggctctgttg acggaattat ggttgaaatc tggagctacc
     6241 atgcgttgtt ctggctggcg atagggatgt tgggtattgc gatgatttgc ctgctgttta
     6301 ttaaagatat ttagctcacc agtctgggct aagaaaaatc ggcgtggcgc aaacagagcc
     6361 atcctcgcca tttttgctat catgcctgca tacataaacg acaaaacagt atgcagaggg
     6421 aaaaatgggt tccaccagaa aggggatgct gaacgttctg attgccgccg tgttgtgggg
     6481 aagttcaggg gtctgcgcgc aatacatcat ggagcaaagc cagatgtcgt cgcagttttt
     6541 gactatgacg cgtttgatat tcgccggttt gattctactg acgctgtcat ttgttcatgg
     6601 cgataaaatc ttttctatta ttaacaatca taaagatgcc attagcctgc tgattttttc
     6661 cgtggttggc gcgctaactg tacagctcac ttttttgcta accatcgaaa aatcgaacgc
     6721 agccacggca acggtgctgc aattcctctc accgacgatt atcgtcgcct ggttctcact
     6781 ggtgcgtaaa tcgcgcccgg gcattctggt tttctgcgct attttgacat cgctggtcgg
     6841 gactttttta ttggtgacac acggtaatcc gacgtcatta tcgatctctc ctgccgcgtt
     6901 gttctggggc attgcctcgg catttgctgc tgcattctat accacctatc cctcaacgct
     6961 aattgcccgc tatggcacgt taccagtcgt cggctggagt atgctgattg gcggtctgat
     7021 tctgttgcct ttttatgcca gacaaggaac aaactttgtc gttaacggca gtttgattct
     7081 ggcgtttttt tatttggtgg tcattggtac gtccctgaca tttagtctgt acctgaaagg
     7141 agcacaatta attggcggtc caaaagccag cattttgagc tgtgcagaac cattaagtag
     7201 cgcgctactc tctttgctgt tgctggggat cacgttcaca ttaccggact ggctgggaac
     7261 gctgctgatt ctgtcatcgg tgattttgat ttcaatggat tcccgtcgcc gcgccagaaa
     7321 aataaatcgt ccggcgcggc atgagtgaga attaccagcc aggcaccgcg ccaccgttaa
     7381 aaatggtttc tgccgcttta gcgacttcgg gtgattgata agattgcaga aattccttca
     7441 cgttttctgc attcttatta tcttcccgtg ccaccaaaat attcacatac ggcgaattct
     7501 tatcttcaat aaatacgctg tcgtgcaccg gagaaagccc tgtctgctga atgtaagtgg
     7561 tactgataat cgctacatca actttgggat catccagtac gcgcggaagc tgcgccccct
     7621 cgagttccat aatctgcaaa tgacgaggat tatcggttat atccagcgcg gtaggtaata
     7681 acccttttcc ctctttcaga gtaattagct tctctttttg caacagtaaa agtgcgcgac
     7741 caaggttggt cgggtcgttg gggatcgcca ctgtcgcgcc ttcttttatt tgcgccacgg
     7801 ttgtgatttt tttggaataa cccgccatag ggaagacaaa cgtatttccc accgctacca
     7861 gtttataacc atgcgcctga ttatcttgtt caagaaaggg tcgatgctgg aagacattcg
     7921 catcaagttc gccatgattt gtcgcatcgt tcggtagcaa cgaaccgcta aatcctacca
     7981 gttcaacatc gaggccatat ttctcttttg ccaccttttt agcgacttcc gcgacatctt
     8041 gttctgcgcc atttataacg ccaactttaa tgtgttttgc atcgctgcta ctctggtcgc
     8101 aacctgccag cagaattccg gccagcaata atgcggcccc tgtccgtaga tgatgtgttg
     8161 tcagtttcat ttttttatcc ttttattaat gattacctga ctatagcggt aaggtcgctg
     8221 cggtttaaaa aacgaaacgc tatcgataag aataaaaagg aataaaagtg gaatataaag
     8281 tctggcactt cttactgacc acgcaagcgc gtttcgtaca gcatgatgag agcgatgaaa
     8341 gcaaactaca cttatgcttc attcgataca catttgtaaa aggttgaatc catgaaacca
     8401 acgacactac tgcttatttt cacttttttc gctatgccag gcatcgtata cgccgagtcg
     8461 ccttttagct cactgcagtc cgcaaaagag aaaaccacgg tattacaaga tttacgtaaa
     8521 atttgcacgc cacaggcgtc attatcagat gaagcgtggg aaaagttaat gttgtcagat
     8581 gaaaacaata aacaacatat acgcgaggcc atcgtggcga tggaacgtaa taatcagagc
     8641 aattattggg aagcgttggg taaggtagaa tgcccggata tgtaagaaca gcaacaacta
     8701 ctgaccaacg cttctttacc tgatatcagg atttcttcat tttcaccttt gcagtaacca
     8761 acaatgcagt cagcaacatc aatgtgccgg acaacatcaa cggcgaagtc agaccaatat
     8821 tatccagcgc ataaccgccg attgccgcgc cacaggtatt agcaagctga ataaccgcca
     8881 cctgaataga cccggctttt tctgcctgat cggccagcga gcgggtgatc cacgttgacc
     8941 agccgacagg aaccaatgca aaagttagcc cccagataat cgccacgccg gtagcaacga
     9001 ttttatcgct tccccacaac gtcagcacca acgcactcac agccagtatt aacggcgcgc
     9061 ctgctaaggc cagttttacc gaacgtttaa gaatgaatga cgaaagcgac gtaccgataa
     9121 agctggcaat gccaaaactc aacagcacca gcgttaagcc atccacgctg aatcccgcca
     9181 ggttcatata caccgggcga atatatgtga agaaagcaaa ctgcccggcg aaagacatga
     9241 agatggcgat catccctgcc atcacacccg gacgttgtaa taagcggaaa gtattttgtt
     9301 tctgatgcga gggttcgcct ggcagtgaag gcaatgattt gataatccag aaaatacaca
     9361 gcacgcccat caccgccgcc gcattaaaga cattgcgcca accgataagc tcgcctaaaa
     9421 aactgcccaa cggcgcggca atcaccagcg caatagaaac cgcgccgaag atcaccgaca
     9481 gcgccttcgg caccgtacgc ggcggcacca gacgcatggt cagcgacgcc gacatcgccc
     9541 agaacccgcc cagcgccagc cccagacagg cacgaccgat taaaagcaaa ctgaatgagt
     9601 tagcaaagga aaccagcaag caggagagcg tcagcaaaac ggcaaacaaa ataacaacgt
     9661 agcggcggtc agtagcctga attgtctggg taataaacaa actggcaaac attgccacaa
     9721 aggcggtcac ggtcaccgat tgcccggcaa ccccttccga aatgcctaaa tcctgggcca
     9781 ttggcgtcaa caaactgacg ggcaaaaact caacgataat cagacaggcg acacaaaacg
     9841 ccaccgagaa aacggctgac cagttcggtc gggtgatggc atccgcgccg cggttttcgg
     9901 caataaattc actcatggtg ttacccgtgg catgtttcag gggaaaagcc gaccacttta
     9961 acattgaaat gtgtgacgca tttaacgttt ttgcaacttt tacggtgttg cttgagctgg
    10021 agttaagatc gaaacggaac agggcaaaga ttaccggatg cggcgtgaac gtcttatcca
    10081 cccgacaggt caggcgcggt ctgtaggctg ataagacgaa acggtttcaa tgcaaccagt
    10141 gaacgccctc ggcaggctga aagaacgcgt tatagcgcat ctgaaaagca ttaatatcct
    10201 gcatttcagg ttcggtttcg cgcagaaaac ctagcgccag cctgacctga gcgtcggtaa
    10261 tcggcgcggc gaggcgagct ttttgtaaaa tccgatgcca ttgcagcagg ttctgttcgc
    10321 taccatcgac aatgacaatt tgccagatga gcaacacctg cgcggcgttt tgcaggtgcg
    10381 actcatccgg acgatgcaaa taacgtaaaa attcgtcacc agctgacttt cccatttgat
    10441 cgaccatcga ctccacgggt atgacatcga tattcagccg atcgctgaga cggcgaatcg
    10501 cttttctggc cccggatgtc agcacccgaa aaccaaccac aaacaccact gccagcgtgg
    10561 cgagcattat ccagatcatg agttctcctg cgtaatcaga actcatgata tctggaaacc
    10621 ctcgccagtt actaatccac cagaataatc ttcagtgcaa acagagctgc gaccaccacc
    10681 acacacaggt tcagatcgcg ccagcgcccg gtgcacactt tcatgatgca gtacgacata
    10741 aagccgagtg caatcccttc ggtgatcgag aaagtaaagg gcatcatcac cgtggtaata
    10801 aacgcaggca ccgattcggt aaaatcatcc cagttaacgc gcgccaggct cgaagtcatc
    10861 agcacgccaa caaagattaa cgctccggcg gttgcgtaag gaggaaccat cgccaccagc
    10921 ggtgagaaga acataaccaa caggaacata acgccaacca caaccgcagt cagccccgtg
    10981 cggccaccga ctgccacacc agaagtactt tcaatatagg cagtaacaga cgaggtgccg
    11041 ataaacgcac ccgccaccga actgacgcta tcaacataca gcgccttatt catattgggg
    11101 aatttaccgt taccatctat taagcccgct ttatcagtta caccaattaa tgttcctgat
    11161 gaatcaaata ggttgatcag cataaaggag aaaatgatac cggcgagttc aagtgttaac
    11221 gcgccgctca aatctacttc accaatgacg ccgctaatat caggcggaat ggaatagacg
    11281 ccgctaaaat gaacatcacc gaaaaataat ccacagcaag acgtcaccac aatagaaacc
    11341 agcaccgcgg catgaaaatg acgtgatgac aacacggtta taataaaaaa ccctaaaata
    11401 cctaacaaca cgccgtgaga acttaaatcg ccaatcatca ccagcgtgtc tttattggcg
    11461 acaataacgc cagtattttt taatcccatt aaggcgataa ataatccaat tccgctggtg
    11521 ataccaatac gtaaacttaa gggaatgttg gagatcatcc agtaccggat acgaaacagc
    11581 gtgagcaaaa atagtccaac tgcgccccag aatattgcgc ccatcccggt ctgccaggag
    11641 atgcccatcg cccccacgac cacgaaggca aagaaggcgt tcagccccat tgccggagcc
    11701 agcgccacgg gtaagttagc aaatatcccc atcgcaatac tgccgatacc ggcaatcaaa
    11761 caggtggtaa caaacaccac tttcgggtcc atttgtgccg cgccgaggat ttgcgggttc
    11821 acaaaaacga tgtacaccat ggttaaaaaa gtggtcatcc ccgcaatcaa ttctgtgcgg
    11881 acggtggtcc catgctgagg tagtttaaat aatcgcgaaa gcgtccctga ttcattactc
    11941 acgtaatcgg tattgtcatt attcattttt ttgtccattg gaggagattt aatcccttgc
    12001 ttttgtaaaa caaatgacaa caacggttca gtgataatta tcacatttca attgcacatt
    12061 aatggatatt ctttaataat ctcgcg
//
LOCUS       DAFNBJ010000142        12001 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000142 DAFNBJ010000000
VERSION     DAFNBJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.142"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            187..890
                     /locus_tag="L2X49_003789"
                     /pseudo
     CDS             187..890
                     /locus_tag="L2X49_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
     gene            complement(1097..1543)
                     /locus_tag="L2X49_003790"
     CDS             complement(1097..1543)
                     /locus_tag="L2X49_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBN4798804.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1635..2294)
                     /locus_tag="L2X49_003791"
     CDS             complement(1635..2294)
                     /locus_tag="L2X49_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBN4798805.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(2366..2659)
                     /locus_tag="L2X49_003792"
     CDS             complement(2366..2659)
                     /locus_tag="L2X49_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBN4798806.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2901..3302
                     /locus_tag="L2X49_003793"
     CDS             2901..3302
                     /locus_tag="L2X49_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBN4798807.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3422..3790)
                     /locus_tag="L2X49_003794"
     CDS             complement(3422..3790)
                     /locus_tag="L2X49_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798808.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4310..5005
                     /gene="minC"
                     /locus_tag="L2X49_003795"
     CDS             4310..5005
                     /gene="minC"
                     /locus_tag="L2X49_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBN4798809.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5029..5841
                     /gene="minD"
                     /locus_tag="L2X49_003796"
     CDS             5029..5841
                     /gene="minD"
                     /locus_tag="L2X49_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBN4798810.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5845..6111
                     /gene="minE"
                     /locus_tag="L2X49_003797"
     CDS             5845..6111
                     /gene="minE"
                     /locus_tag="L2X49_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBN4798811.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6483..6827)
                     /locus_tag="L2X49_003798"
                     /pseudo
     CDS             complement(6483..6827)
                     /locus_tag="L2X49_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6862..6981)
                     /locus_tag="L2X49_003799"
     CDS             complement(6862..6981)
                     /locus_tag="L2X49_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBN4798812.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6942..7127)
                     /locus_tag="L2X49_003800"
     CDS             complement(6942..7127)
                     /locus_tag="L2X49_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798813.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7228..7401
                     /locus_tag="L2X49_003801"
     CDS             7228..7401
                     /locus_tag="L2X49_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798814.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7403..7747
                     /locus_tag="L2X49_003802"
     CDS             7403..7747
                     /locus_tag="L2X49_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBN4798815.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7751..8086
                     /locus_tag="L2X49_003803"
     CDS             7751..8086
                     /locus_tag="L2X49_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBN4798816.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8143..10464)
                     /locus_tag="L2X49_003804"
     CDS             complement(8143..10464)
                     /locus_tag="L2X49_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBN4798817.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10581..10790)
                     /locus_tag="L2X49_003805"
     CDS             complement(10581..10790)
                     /locus_tag="L2X49_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798818.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(11190..11408)
                     /locus_tag="L2X49_003806"
     CDS             complement(11190..11408)
                     /locus_tag="L2X49_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798819.1"
                     /translation="MNNSNNLDYFTIYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(11540..>12001)
                     /locus_tag="L2X49_003807"
     CDS             complement(11540..>12001)
                     /locus_tag="L2X49_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN4798820.1"
                     /translation="CLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNILHENGFV
                     IALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLARKLSISI
                     VAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVAVE"
BASE COUNT         3405 a         2823 c         2590 g         3183 t
ORIGIN      
        1 aaaacggtag aagtagttaa aaacgcaatc gaaactgcag atggagcatt agatctttat
       61 aataaatatc tcgatcaggt catcccctgg cagacctttg atgaaaccat aaaagagtta
      121 agtcgcttta aacaggagta ttcacaggca gcctccgttt tagtcggcga tattaaaacc
      181 ttacttatgg atagccagga taagtatttt gaagcaaccc aaacagtgta tgaatggtgt
      241 ggtgttgcga cgcaattgct cgcagcgtat atttttctat ttgatgagta caatgagaag
      301 aaagcatccg cccagaaaga cattctcatt aaggtactgg atgacggcat cacgaagctg
      361 aatgaagcgc aaaaatccct gctggtaagc tcacaaagtt tcaacaacgc ttccgggaaa
      421 ctgctggcgt tagatagcca gttaaccaat gatttttcag aaaaaagcag cttttccagt
      481 cacaggtaga taaaatcagg aaggaagcgt atgccggtgc cgcagccggt gtcgtcgccg
      541 gtccatttgg attaatcatt tcctattcta ttgctgcggg cgtagttgaa gggaaactga
      601 ttccagaatt gaagaacaag ttaaaatctg tgcagaattt ctttaccacc ctgtctaaca
      661 cggttaaaca agcgaataaa gatatcgatg ccgccaaatt gaaattaacc accgaaatag
      721 ccgccatcgg ggagataaaa acgggaactg aaacaaccag attctatgtt gattatgatg
      781 atttaatgct ttctttgcta aaagaagcgg caaaaaaaat gattaacacc tgtaatgagt
      841 atcagaaaag acacggtaaa aagacactct ttgaggtacc tgaagtctga taagcgatta
      901 ttctctccat gtactcaagg tataaggttt atcacattta aaaacacaga cgttttttaa
      961 agattgagag ttacctgttg ataataaggt atcgggtaac tctcattatg acagagcgct
     1021 ttcaaaaaca gggctacgaa atggcgagca tccagacgta tattaagaaa gcccccatca
     1081 acatctatta aatttatcac tgcgcccagt caggtttatt taagatatga tcctgccagt
     1141 caatcacttc tgattctttc actgcgatat gacgcacaga gatacgttca ccatgcattg
     1201 ccgctttcga acccgtaagt agcggatgcc acgcaggtaa atctttacct tccgccagca
     1261 accgataagc gcaggtcatt ggcagccatt cgaatgttgg cagattttca cgggttaatt
     1321 taatgcagtc gggttcaaac tcgaaacgac gttcgtagtt ccgacattga caggttttaa
     1381 tattgagctg gcgacaggcg acgttagtga agtagatttc gtcggtgtct tcatccatca
     1441 gtttatgcag gcaacactga ccgcaaccat cacacaacga ctcccattcc gcatcgctca
     1501 tttcgtccag ggttttactt tgccagaaag gtacatcgct catcaggtgt tccgccatta
     1561 cgttaaaacg caccttataa ccagtctggc acagcgatgc aagttttgcc gccgctttca
     1621 ggcggcaaaa agtattacaa aacgcgagtt gccaaagaat gcccatcgaa agtgactgtc
     1681 agctcatcac cgctttgcaa cgggccgacg ccatcaggcg tgcctgtcag cacaacgtca
     1741 ccggccttga gggtaaaaaa cttgctcata taagcgatca gcggaacaat tttatggatc
     1801 atgtccgcag tcgtaccttg ctgacgttgc tcgccgttta cgctcaggcc cagcgttgta
     1861 ttttgcgggt cgccggtaaa ttccgccgcg ggaataaacc cggaaagcgg acaagagtta
     1921 tcaaacgctt tagccttttc ccacggctgc ccggctttct tcatttttcc ctgaacatca
     1981 cgcaatgtca gatcgagcgc cacgccataa ccggcaatgg ctttgcggac atgctcttcc
     2041 gtagcctgac gcagcgtcgc gccaatcaac accgccagtt cgacttcatg atgaactgaa
     2101 ccgaaatcgg atgggatcgc caatggctgc cgcagatcgc acagtgccgt ttctggttta
     2161 ataaacagca ctggctcttc gggcactgcg ctgcccatct ctttaatatg tttggcatag
     2221 ttactgccaa cacagactac tttactcacc ggataatcca gcagcgcacc ttgccagttg
     2281 tgatgttgat acataacgtt cccctcacct tatcgtggat atgaaaaccc gcgccgacaa
     2341 agataatgcc accgccggat atcggttatt tgttagtgtc gtctgttttc tgccccatga
     2401 cggaaagatg ttgcttcagc aaatcttcgg gtggtggcgg taactgcaaa tagtaacctt
     2461 gctcggttaa tgccagttta accttttcaa tatcagcatt gaccagtttc ttccgcccat
     2521 ccaacggcag aatcatcgct aactgaggct gaccaaaacc tttcatcagt tcctcaggaa
     2581 cacgcgaaaa atcgtctttt ttttcgacat ataaataggt ctggtcacgc ttgctgcttc
     2641 gataaatcac acaaaacata cttttactct ttaagatgcc aggttttggc ataagctaac
     2701 atcaatgaaa cccgcgccac aagccagcca gctgtagatt tctacggttt gttgagtcca
     2761 tgccccttta taagtaatcc ccccggcaaa aaatgccgcg ccgggatttt accgaacgca
     2821 gaaaagccac tggaattctc ccgtaaggga ttgttatatt ataacagttc atcgtactca
     2881 ttctgaacag gagactacca atgaaaatca agagcatcag taaggctgta ttgctgcttg
     2941 cactgctaac ctccacgagc tttgctgcgg gtaagaatgt caatgttgag ttcagcaaag
     3001 gccatagcag cgctcagtac tccggcgaaa taaagggata cgattacgat acatatacct
     3061 tctatgccaa aaaaggtcag aaagtgcatg tgagtatctc taatgaaggc gctgatactt
     3121 acctcttcgg gccaggaatc gatgattcag ttgatttatc cagatattca ccagaactcg
     3181 atagtcacgg acaatactca ctccctgctt ctggcaaata tgagctgcga gtgctgcaaa
     3241 cccgaaacga cgcccgtaaa aacaaaacta aaaaatataa cgtcgatatt caaattaagt
     3301 aaatgtcaac cgacggggac tctccccgtc ataaacgtta acgccccact cctctctgtc
     3361 gctcaagagg ttaatgacga atagcagaca atgccgaata gaacataatg taaacaaggc
     3421 atcactcacg acacataaat aacatctttg tggtgatttg attgattttt ccactccgct
     3481 taccatccac accaaaataa cgatcatcac gacataattt ttctttaaca tcgcaaaata
     3541 caccgttagc gaatgtaaat tcgctgggat cgtagccttg taatgattgt aagttttcag
     3601 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
     3661 aaatatacgg gtcgcaaaca acgccgcacg ccggagattg aagtccgcca gcgtcatgtc
     3721 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa aaaatacgca
     3781 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcatcatc
     3841 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
     3901 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
     3961 gttctttaat gatgccgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
     4021 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
     4081 gaaaaaggaa gcgtaaataa gaacaatcaa aagtacgacg gcaatgggtt gattgacaag
     4141 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
     4201 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
     4261 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
     4321 gccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
     4381 acctaaggtt atccatcagg cgctggaaga caaaatcgct caggcccccg catttttaaa
     4441 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
     4501 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
     4561 gcaacttaaa gccgaaattg aaaagatggg gctgcctatc ctgacggaag gaaaggaaaa
     4621 agcgccgcgt ccagctcccg caccacaggc tccagcgcaa aatacaacgc cggtcacaaa
     4681 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     4741 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     4801 tgtctatggc atgatgcgcg gtcgtgcgct tgcaggggca agtggtgacc gggaaacgca
     4861 aatattttgt acgaacctga tggcggaact ggtgtccatc gcaggtgaat actggctgag
     4921 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     4981 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     5041 attgttgtta cttcgggcaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     5101 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     5161 aatctcgacc tgattatggg ttgtgaacgc cgggtcgttt acgatttcgt caacgtcatt
     5221 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     5281 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     5341 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     5401 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     5461 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcgaaatca
     5521 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     5581 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     5641 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     5701 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     5761 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     5821 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaaa
     5881 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     5941 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     6001 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     6061 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     6121 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     6181 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     6241 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     6301 aaataaccct gttgttagtg tggatatact gggagatgat aaccccgcaa tttcattatt
     6361 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     6421 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     6481 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     6541 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     6601 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     6661 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     6721 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     6781 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     6841 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     6901 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     6961 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     7021 atcactcttt tcagataata ctgtcgtacg cttatctttc gacaatgagg ccgtcggcaa
     7081 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     7141 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     7201 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     7261 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     7321 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     7381 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     7441 attgttctgc tccacgccag cgatggcgga tatgaatcgc accacgaaag gtgcattgct
     7501 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     7561 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     7621 aggtgcaaaa gtaggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     7681 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     7741 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     7801 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     7861 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     7921 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     7981 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     8041 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     8101 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     8161 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     8221 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     8281 actcgcccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     8341 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     8401 ctcctgagta cgaataaagg tgcggaaacc aagtcgggtc tggatgttgt tatttgcgtc
     8461 attctcaata cgtgagccat tggcttcggt gtgcgcatta tgatcgacgc ccatccagat
     8521 agcctgtgcc tgtggctgga atatccacgt gtatttcgcc gcttccagac caaaggattt
     8581 attcagggca aagccgtaac ccgcttccag cgagacggtt gcgcctttcg cactatagga
     8641 ttcataggct aattcatccc ctttcaccga gtttttaaac cagctatatt gcgcccaggc
     8701 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     8761 gcaatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttgagtt
     8821 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactgt cggtaaaacc
     8881 accggttaac agctcggccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     8941 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     9001 acgctcctgt tcagttacag gatcgatgta gcgcatttca cccgcacggt cgttcagacg
     9061 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcgaggcg
     9121 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     9181 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     9241 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     9301 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     9361 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     9421 cgtagctgaa ttatcatcac ccaattcagt tctgagatgc aatacgccat tattaccaac
     9481 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     9541 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     9601 ggtactatta tctgaaagcg acagataggt atgagtgtta acatctgttg aaatattagc
     9661 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     9721 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     9781 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     9841 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     9901 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcagtcg cggcgtcagg
     9961 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
    10021 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
    10081 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
    10141 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
    10201 attacttcct gtatcggtag tactttcttc atcaagagaa gatacacacg cacgcgcgct
    10261 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
    10321 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
    10381 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttat
    10441 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
    10501 agctgttaat gctgtgctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
    10561 aatgacgcca ttgttaatgg ttaacttgct accctctcct gcaacggata cccgtaactt
    10621 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
    10681 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
    10741 gccagagcaa taagtcccaa actaaacccg gagagttttt tcagtttcat agattaacca
    10801 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgcgttca gattaccaaa
    10861 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
    10921 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
    10981 tttagggtga cgattttgtt taacaaataa ctcttcgaca tcaaaattgt taatagttgc
    11041 gatgaggatt atttcggtga tatataaaaa caaccgtgat aacaccaggg ttgacaacta
    11101 tttgcgcaat aatgcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
    11161 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggtt gacgagtctt
    11221 tctcttaatc gattaaccag attgatcgcc agccaaacaa gggcattaag caaatttatc
    11281 gtcacaagca cttctcccag cccggtactg ggttttgcaa taaggatgac caggagggtg
    11341 atcagcataa atgcaatgga aaagatgata tagatagtga aataatccag attattacta
    11401 ttgttcatgc tactaatggc aatcctgtca aaactttcaa acaattatag caaacaaaat
    11461 aatgaacaat agtaacaaga ggcaacgagc taattagctg tctataatta caccgcacta
    11521 tggccttact gatcaatttt cactcaaccg caaccttcac cttcggttta gaaaggataa
    11581 ttttgaccag gtcgatgtat gtaacaggtt tatagaaata ataaccctgc tgaaatgtga
    11641 tattattttg gttcagatag tcaagttgtt ctttcgtttc gacaccttca gcgacgatac
    11701 tgatcgaaag tttacgcgcc agatccaata cacaatccag aattcgggtt gattctgggt
    11761 ctgcgtttac gcggccaacg aaactgtgat caattttgat ataatcaata tgcaggtcat
    11821 gaagataaga aagccctgag tagccagtac cgaaatcatc cagcgcgatg acaaaaccat
    11881 tttcatgcag aatgttcaac cgctgaacca gactttcatc aacattcaat ggctcacgct
    11941 cggtgacttc cagaacaagg tttaaatcgc ggcgggtaaa actgtcacgg aaatttaaac
    12001 a
//
LOCUS       DAFNBJ010000143        11915 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000143 DAFNBJ010000000
VERSION     DAFNBJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.143"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..248)
                     /locus_tag="L2X49_003808"
     CDS             complement(<1..248)
                     /locus_tag="L2X49_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBN4798821.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            305..862
                     /locus_tag="L2X49_003809"
     CDS             305..862
                     /locus_tag="L2X49_003809"
                     /inference="COORDINATES: protein motif:HMM:NF023351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HBN4798822.1"
                     /translation="MWITIAPFVTQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGEND
                     TRFAVDFTWQPGSAMQKQLDPNEVAARRSLASSRYDLVDRNNNIVLEYRKKELVRLSL
                     TDPVTGKSGEVKSLVSSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFT
                     STPETDNTWPIEVTAEDVKGNLFES"
     gene            870..7289
                     /locus_tag="L2X49_003810"
     CDS             870..7289
                     /locus_tag="L2X49_003810"
                     /inference="COORDINATES: protein motif:HMM:NF020698.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HBN4798823.1"
                     /translation="MVVVQAPTLSQKDSSVSLSTQTLSADSHSTATLTFIAHDAAGNP
                     VIGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPA
                     VVNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDN
                     VKPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAK
                     IATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKT
                     DVNGLATIDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADG
                     NDSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDY
                     TVMASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQHMTATLQDKNGNPL
                     KDKEITFSVPNDVASRFSISNGGKGMTDSNGVAIASLTGTLAGTHMITARLANSNVSD
                     AQPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPA
                     DTQLSLNARNTNENGITEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTL
                     NITAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTIPQDVAANFTLENNGIAITQA
                     NGEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGV
                     DETTLTATVKDPFDNVVKDLPVTFSTNPADTQLSQSTSNTNDSGVAEVTLKGTVLGVH
                     TVEATLLNGNGYTTTVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPV
                     AGITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSD
                     SQPVTFVADKASAQVVLQISKDEITGNGVDSATLTATVKDQFDNEVNNLPVTFSSASS
                     GLTLTPGVSNTNESGIAQATLAGVAFGEKTVTASLANNGASDNKTVHFIGDTAAAKII
                     ELTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVT
                     NEQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQAL
                     FDTVSAGETTSLYIEVKNNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYAS
                     FTATKAGVYQLTATLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTAT
                     VADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAVTDTEGKAKVTLKGTKAGAHTVTA
                     SMAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEA
                     VTFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGSYPVTVSVNNYGVSDTKQV
                     TLIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVSFRGTSVTLSS
                     TSVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNASADVNSATITSLEIP
                     EGQVMVAQDVAVKAHVNDQFGNPVAHQPVTFSAEPPEHMTISQNIVSTDTHGIAEVSM
                     TPERNGSYMVKASLANGASLEKQLEAIDEKLTLTASSPLIGVYAPTGTTLTATLTSAN
                     GTPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQ
                     TQTTVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALK
                     SGSATLTSLTAVTDQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADASQ
                     SVLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGMEFVQSGTNVPYMKISAIDYS
                     QNINGDYKATITGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDL
                     PTTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDAIGKVTFKNVGSNWE
                     RITATPKSGGPSYVYEIRVKSWWVNAGEAFMIYSLAENFCSSNGYTLPRANYLNHSSS
                     RGIGSLYSEWGDMGHYTTEAGFQSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAY
                     ATCYKNL"
     gene            complement(7551..8603)
                     /locus_tag="L2X49_003811"
     CDS             complement(7551..8603)
                     /locus_tag="L2X49_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HBN4798824.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNVLKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     NLRAPVPMTWAFMIREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIAHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITHDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            8918..10234
                     /gene="shiA"
                     /locus_tag="L2X49_003812"
     CDS             8918..10234
                     /gene="shiA"
                     /locus_tag="L2X49_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HBN4798825.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNHRA"
     gene            10336..11790
                     /gene="amn"
                     /locus_tag="L2X49_003813"
     CDS             10336..11790
                     /gene="amn"
                     /locus_tag="L2X49_003813"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBN4798826.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         3199 a         2905 c         2880 g         2931 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacc gtattgcggg agatccccag ttctctggca
      181 atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacacaaagt aatcctcatg ccataaccgc
      361 tggacttaac tataccccct tcccgctgat gaccttcagc gcggagcaac gtcagggtaa
      421 acagggcgaa aatgacaccc gttttgccgt cgattttacc tggcaacctg gcagcgcaat
      481 gcagaaacag cttgacccga atgaagtcgc tgcacggcgt agccttgcaa gcagccgtta
      541 tgatctggtg gatcgcaaca acaacatcgt tctggaatat cgcaaaaaag aactggttcg
      601 cctgtccctg acagaccccg tgacagggaa gtcaggagaa gtgaaatcac tggtttcgtc
      661 gctacaaacc aaatatgccc tgaaaggcta taacgtcgaa gccaccgctc tggaagctgc
      721 cggtggtaaa gtggttacaa cgggtaaaga tattctggtt accctgccgg cgtaccggtt
      781 caccagtacg ccagaaaccg ataacacctg gccgattgaa gtcaccgctg aagatgtcaa
      841 aggcaatttg ttcgaatcgt gaacagagca tggtagtcgt tcaggcacct acgttaagcc
      901 agaaagattc ctcggtatcg ttaagtaccc agacgttgag cgcggattcc cattcaaccg
      961 ccacactgac ttttattgcg catgatgcag caggtaatcc tgttatcggg ctggtgctct
     1021 cgacgcgtca cgaaggtgtt caggacatca ccctttctga ctggaaagat aatggtgacg
     1081 gaagctatac ccagatcctg accacaggtg cgatgtctgg cacgctgacg cttatgccac
     1141 agctgaacgg tgtggatgcg gctaaagccc ccgccgtggt gaatatcatt tctgtttcgt
     1201 catcccggac tcactcgtca attaagattg ataaggaccg ttatctctcc ggcaatccta
     1261 tcgaggtaac ggtagaactg agagatgaaa atgacaaacc tgttaaggag caaaaacagc
     1321 aactgaatac cgcagtcagc atcgacaatg tgaaacctgg tgtcactaca gactggaaag
     1381 aaaccgcaga tggcgtctat aaggcaacct ataccgccta taccaaaggc agtgggctta
     1441 ctgcgaagct attaatgcaa aactggaatg aagatttgca taccgctgga tttatcatcg
     1501 acgccaaccc gcagtcagcg aaaattgcga cattatctgc cagcaataat ggtgtgctcg
     1561 ccaatgagaa tgcagcaaac accgtctcgg tcaatgtcgc tgatgaagga agcaacccaa
     1621 tcaatgatca taccgtcacg tttgcggtat taagcggatc ggcaacttcc tttaacaatc
     1681 aaaacaccgc aaaaacggat gttaatggtc tggcgactat tgatctgaaa agtagtaagc
     1741 aggaagacaa cacggttgaa gtcacccttg aaaatggcgt gaaacaaacg ttaatcgtca
     1801 gttttgtcgg cgactcgagt accgcacagg ttgatctgca gaagtcgaaa aatgaagtgg
     1861 tcgctgacgg caatgacagc gccacaatga ccgcgacagt tcgggatgca aaaggcaacc
     1921 tgctcaatga cgtcaaggtc accttcaatg ttaattcagc agcagcgaaa ctgagccaaa
     1981 ccgaagtgaa tagccacgac gggatcgcca cagctacgct gaccagtttg aaaaatggtg
     2041 attatacggt tatggcctct gtgagctctg gttctcaggc taatcaacag gtgaatttta
     2101 tcggtgatca aagtactgca gccctgaccc tcagtgtgcc ttcaggtgat atcaccgtca
     2161 ccaacacagc tccgcaacat atgactgcaa ccttgcagga taaaaatggc aacccactaa
     2221 aagataaaga aatcaccttc tctgtgccaa acgacgtcgc aagtcggttc tcgattagca
     2281 acggcggaaa aggcatgacg gatagtaacg gggttgcaat cgcctccctg accggcacgt
     2341 tagcgggcac gcatatgatc acggctcgtc tggctaacag caatgtcagc gatgcacagc
     2401 caatgacgtt tgtggcggat aaagacagag cggttgtcgt tctacaaaca tcgaaagcgg
     2461 aaatcattgg gaatggtgtg gatgagacga ccctgacagc aacagtgaaa gatccttttg
     2521 ataacgtggt taaaaatcta tcagtagtct tccgcacctc ccccgcagac acgcaactga
     2581 gtctgaacgc gcgtaatact aatgagaacg gtattactga agttaccctt aagggcacgg
     2641 ttttgggtgt tcatacagcc gaagccatac tgcttaacgg caacagagat acgaagatag
     2701 tcaatattgc gcccgatgcc agcaacgcgc aggtcaccct gaacatcact gcacaacagg
     2761 tggtgacgaa taacagcgac agcgtgcagc tgacggcgac ggtgaaagat ccgtcgaatc
     2821 atccggtggc gggaataacg gtgaacttca ccataccaca ggacgtggcg gcaaacttta
     2881 cccttgaaaa taacggtatt gccatcactc aggccaatgg cgaagcgcat gtcaccctca
     2941 aaggcaaaaa agcgggcacg catacggtta ccgcaacgct gggtaacaat aatgccagcg
     3001 atgcgcaacc agtcaccttc gtggcggata aggacagcgc ggttgtcgtt ctgcaaacat
     3061 cgaaagcgga aatcattggg aatggcgtgg atgagacgac tctgacggca acagtgaaag
     3121 atccttttga taacgtagta aaagatctac cggtcacctt cagtaccaac ccggcagata
     3181 ctcaacttag tcagagcaca agcaatacta acgacagtgg tgttgctgaa gttaccctta
     3241 agggcacggt tttgggtgtt catacagtcg aagccacgct gcttaacggc aatggctata
     3301 cgacgacagt caatattgcg cccgatgcca gcaacgcgca ggtcaccctg aacatccctg
     3361 ctcaacaggt ggtgacgaat aacagtgaca gcgtgcagct tacggcgatg gtgaaagatc
     3421 cgtcgaatca tccggtggcg gggataacgg tcaacttcac catgccacag gacgttgcgg
     3481 caaactttac ccttgaaaat aacggtattg ccatcaccca ggccaatggg gaagcgcatg
     3541 tcacgctgaa aggtaaaaaa gcgggcacgc atacggttac cgcaacgctg ggtaataaca
     3601 ataccagtga ttcgcagccg gtaacatttg tggcggacaa agcctcggct caggttgttc
     3661 tgcagatatc aaaagatgag atcacaggta atggcgtcga tagcgcaacg ctaactgcaa
     3721 cggttaaaga tcagttcgac aatgaggtga ataatcttcc ggtaacattc agctcagcct
     3781 cttcaggact caccctgacc ccgggagtaa gtaataccaa cgagtctggc atcgcgcagg
     3841 ccactctcgc aggcgttgcc tttggtgaga agacggttac tgcatcactg gctaataatg
     3901 gtgccagcga caacaaaact gtgcatttta ttggcgacac agcggcggca aaaattatcg
     3961 agttgacgcc tgtcccagac agcataatcg ccggtacccc gcagaacagc tccggcagcg
     4021 tcatcaccgc cacagtcgtt gataataatg gctttccggt gaaaggtgtg actgtgaact
     4081 tcaccagcaa cgcagcgaca gccgaaatga cgaacggcgg tcaagccgtg acgaacgaac
     4141 agggtaaggc taccgtcact tataccaata cccgctcctc gatagaatca ggagcgagac
     4201 cggataccgt tgaggccagt ctggaaaatg gtagctccac gcttagcaca tcaattaatg
     4261 tcaacgctga tgcgtctacg gcacatctca ccttgctaca ggcacttttt gatacagtct
     4321 ccgcaggcga gacaaccagt ctgtatattg aggtgaagaa taattacggc aacggtgtcc
     4381 cccagcagga ggtaaccctc agcgtttcac caagtgaagg cgtgaccccc agtaataacg
     4441 ctatatatac taccaaccac gacggcaatt tttacgcaag ctttaccgct acaaaagccg
     4501 gggtttatca attgacggca accctcgaaa atggcgattc gatgcaacaa acagtgacct
     4561 atgtgccgaa cgtcgcgaat gctgaaatca cgctggcagc ctcgaaggat ccggtgattg
     4621 ccgacaataa cgatctcacg acactaacag caacagtcgc tgatacagag ggcaatgcga
     4681 tagccaacac tgaggtaaca tttactctgc cggaagatgt gaaggcgaac ttcacgctga
     4741 gcgatggcgg taaagcggtt actgatactg aaggcaaagc gaaagtcacg ctgaaaggta
     4801 caaaagcagg cgctcatact gttacagcat cgatggctgg cggtaagagt gagcagttgg
     4861 tggtgaattt tattgcggat acgctcacag cgcaggttaa tcttaacgtt accgaggaca
     4921 actttatcgc taataacgtc gggatgacca ggctgcaggc aacagtgact gatggaaacg
     4981 gcaacccgtt agccaatgag gcggtgacat tcacgctacc ggcagatgtg agcgcaagct
     5041 ttactctcgg acaaggcggt tccgccatta ctgacatcaa cggcaaggct gaagttacac
     5101 tgagcggtac aaaatccggc tcctaccccg tgacagttag cgtgaacaat tatggtgtca
     5161 gtgatacgaa acaggtgact ttgattgccg atgctggtac cgcaaaacta gcctccttaa
     5221 cctctgtata ctcattcgtc gtcagcacga ccgagggcgc gaccatgact gcaagcgtca
     5281 ctgacgctaa cggcaacccg gtagaaggca taaaagttag tttccgcgga acctccgtca
     5341 cgctaagcag caccagcgtt gaaacggatg atcggggttt cgctgaaatt cttgtgacaa
     5401 gcaccgaggt cggactgaaa acagtttcag cctctctggc agataaacct actgaagtca
     5461 tctcgcgatt actgaatgcc agtgcagatg ttaattctgc gacgattacc agtctggaga
     5521 taccggaagg tcaggtaatg gtcgcacaag acgtagcagt taaagctcac gttaacgacc
     5581 agtttggcaa cccggttgcg catcaacccg tgacattcag tgcagagcca ccagagcaca
     5641 tgaccatcag ccaaaatatt gtctcaactg atacgcatgg tatagccgag gtctccatga
     5701 cgcccgaaag aaacggttcg tatatggtga aagcatccct ggcgaatgga gcctcacttg
     5761 agaaacaact ggaggctatt gatgaaaaac tgacactcac ggcgtccagt ccgcttatcg
     5821 gtgtctatgc ccctacaggc actactctga cggcaacgct aacctctgca aatggcactc
     5881 cagtggaggg tcaggtcatc aactttagcg taacgccaga aggggcgacg ttaagtggcg
     5941 gaaaagtgag aactaactct tcaggtcagg ctccggtcgt tctgaccagc aataaagtcg
     6001 gtacatatac ggtgactgca tcgttccata acggcgtaac aatacagaca cagacaaccg
     6061 tgaaagtcac tggcaactca agcaccgcac atgttgctag ctttatcgct gatccatcga
     6121 ctatcgccgc caccaacagt gatttaagta ccttaaaggc aacggttgag gatggcagtg
     6181 gtaacctgat cgaaggtctc actgtgtact tcgccttaaa aagcggctct gccacattaa
     6241 cgtcattaac agcggtgacc gatcaaaacg gaatcgcgac aacaagcgtg aaaggagcga
     6301 tgacaggtag cgtcacggta agcgcagtca cgaccgctgg tggaatgcaa acagtagata
     6361 taacgctggt ggcaggcccg gcagacgcct cgcagtccgt ccttaagaac aatcggtcct
     6421 cactgaaagg ggactttacc gatagtgctg agctacatct tgttttgcac gatatatcag
     6481 gcaatccgat caaagtttct gaagggatgg aatttgtgca atcaggtact aacgtgccct
     6541 atatgaaaat tagcgcaatt gattacagtc aaaatatcaa cggtgattat aaagccacca
     6601 ttacaggcgg cggagagggt atcgcaacgc tgatccctgt attgaatggt gttcatcaag
     6661 ctggtctgag taccacaata caattcactc gcgcagaaga caaaataatg agcggtacag
     6721 tatcagtcaa tggtactgac ctaccgacaa ctacattccc ttcgcagggg ttcaccgggg
     6781 cgtattatca gttgaataat gacaactttg ccccaggaaa aacggcggct gattatgagt
     6841 tttcaagctc tgcctcctgg gtcgatgttg atgctatcgg taaagtgaca tttaaaaatg
     6901 tcggcagcaa ttgggaaagg attacggcga cgccaaaatc aggaggccct agctatgtat
     6961 acgaaatccg tgtgaagagt tggtgggtga acgccggcga ggctttcatg atatacagcc
     7021 ttgctgaaaa tttttgcagc agcaatggct acacgctccc cagagcaaac tatttaaacc
     7081 acagtagttc ccgaggcatc gggtcactgt acagtgaatg gggagatatg gggcattaca
     7141 cgactgaagc tggttttcaa tcaaatatgt attggtcatc tagtcccgca aactcaaacg
     7201 aacaatacgt agtttccctg gcaacaggtg atcaaagcgt atttgaaaag cttgggtttg
     7261 cttatgcgac atgttataaa aacctgtgat tttccgttat aatttcttaa agagtgctcc
     7321 attcggagca ctctttttta cattccctct atagagcttg cccttctctc acagaaaatg
     7381 aataaataaa actgctgcac cgcaagaatc gcgttcgatg tgactatgat cgtatccggt
     7441 gagtacagct ttgatccaaa ggctggtttt ctgtgatgat tgaccagcct cagagtattg
     7501 atgacaccac cgttcataac agatcggcat tactatgcat aagtactgtc tcatattctc
     7561 gccaaaacac tttttaatgc ctttgcgaat tcatccagac ttatattgtc aattttttgc
     7621 ccaacgacga ctgactccga aacatcggcc attgcgggcc atcgcgtttt gtaatcagca
     7681 taaattgcaa caacgggggt attataggca cttgccatat gtaatattga agtgtcatga
     7741 gttattgcga tataagcatc ttttatgatt gctgctgaac gtttaatcga gggtgataag
     7801 gataaacggt agacattatt ataacaatcc actaataccc tggctttatc ttcccctttt
     7861 ggtccatgag cgatcactat tggtatatct gtttcacttt gaatttttgc tattagattt
     7921 tctgcaatcg atagtgaaaa tgtacgttcc tgcgagctac cttctaaatt aagcgcaatg
     7981 taagatccta acgatcgcat ttcctcgcgc acctcatcga gtacatcctc acttagtggc
     8041 aattcatata ttggcctgac tgctggaaaa cccgcctcac gaatcataaa tgcccatgtc
     8101 atgggtacgg gagcccggag attctgatcc atccgggacg cgttcttaca cacaggggaa
     8161 tagcacttca tggttaaacc aacaacctga aaattcgttt ttgctttcaa ctgactgata
     8221 aataacatcg ttttcaggtt ctttttacgc atcgcctcta tacaaagatc cggcgtaccg
     8281 tattgctgtg ttatgttctt cgctaaatct tttatttctt ttaatgttgc gtgatcctgc
     8341 atagtcattg tgactaatgt taatttagtc tgttcaagtt tgagcacatt aaagacttct
     8401 aaattaattg tcgacgtaac aattaataga tgcttaattt tatgcaattc aagcgcccga
     8461 ataacaggaa agatggccat agcatcgcca atctgatcgg gaatatggat gacaacaaag
     8521 tctgtttttt caatattgaa attataagct ttataatcgt agtaactaaa tgcaatacgt
     8581 ctcaacaatg atgctaaaaa catacctaac ctcgcctccc tactggttat aatgcaatgc
     8641 agtctatcag actcatcagg gtgccatttt gtgcatatgc ggacttttat gtttcatatc
     8701 tctaacctgt gggtcctctg cttaatcctt aaacaacacc agcaactcct gcgctttcat
     8761 cttccatcga atttttcatg ttgccgctaa tcagccataa aatcatttgc agatgcgctc
     8821 tgtcgaggta gtctcataag gttcgtttat agatcgacgg caatgtgagt taccttttcc
     8881 atactaatta taaaaagaca gtacaaacag gatcattatg gactccacgc tcatctccac
     8941 tcgtcccgat gaaaggacgg tttcgttaag tcgcgcccga cgagctgcgt taggcagctt
     9001 cgctggtgcc gtcgtcgact ggtatgattt tttactctat ggcatcaccg ccgcactggt
     9061 gtttaatcgc gagtttttcc cgcaagtaag cccggcgatg ggaacgctcg ccgcatttgc
     9121 cacctttggc gtcggattcc ttttccgtcc gctcggcggt gtcattttcg gtcactttgg
     9181 cgaccggctg ggacgtaagc gcatgttaat gctgaccgtc tggatgatgg gcatcgcgac
     9241 agccttgatt ggtattcttc cttcattctc gaccattggg tggtgggcac ctattttgct
     9301 ggtgacactg cgtgccattc agggatttgc cgtcggcggc gaatggggag gcgcggcgtt
     9361 gctttccgtt gaaagtgcac cgaaaaataa aaaagccttt tacagtagcg gtgtacaagt
     9421 tggctacggt gtaggtttac tgctttcaac cggactggtt tcattgatca gtatgatgac
     9481 gactgacgaa cagtttttaa gctggggctg gcgcattcct ttcctgttta gcatcgtact
     9541 ggtactggga gcattgtggg tgcgcaatgg catggaggag tccgcggaat ttgaacaaca
     9601 gcaacattat caagctgccg cgaaaaaacg catcccggtt atcgaagcgc tgttacgaca
     9661 tcccggtgct ttcctgaaga ttattgcgct acgactgtgc gaattgctga cgatgtacat
     9721 cgttactgcc tttgcactta attattcaac ccagaatatg gggttaccgc gcgaactttt
     9781 cctcaatatt ggtttgctgg taggtggatt aagctgcctg acaattccct gttttgcctg
     9841 gcttgccgat cgttttggtc gccgcagggt ttatatcaca ggtgcgttaa tcggaacgtt
     9901 gagcgcattt cctttcttta tggcgcttga agcacaatct attttctgga tagttttctt
     9961 ctccataatg ctggcaaaca ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt
    10021 taccgaaatg tttggtgcca gttatcgcta tagtggtgct ggagtcggtt atcaggtcgc
    10081 cagtgtggtt ggcggtggat ttacaccttt tattgccgct gcgctcatca cttactttgc
    10141 cgggaactgg catagcgtcg ccatttattt gctggctgga tgtctgattt ccgcaatgac
    10201 cgctttgttg atgaaagaca atcaccgcgc ttgatagcct ggcgaagatc atccgatctt
    10261 cgccttacac ttttgtttca cattcctgtg acatactatc ggatgtgcgg taattgtatg
    10321 gaacaggaga cacacatgaa taataagggc tccggtctga ccccagctca ggcactggat
    10381 aaactcgacg cgctgtatga gcaatctgta gtcgcattac gcaacgccat tggcaactat
    10441 attacaagtg gcgaattacc tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca
    10501 tcactgaccg taacctggga cggtagcaca accaatcccc ccaaaacgcg cgcatttggt
    10561 cgttttactc acgcaggcag ctacaccacc acgattactc gccctactct ctttcgttcg
    10621 tatctcaatg aacaacttac gttgctgtat caggattatg gtgcgcatat ctcggtgcaa
    10681 ccctcgcagc atgaaatccc ctatccttat gtcatcgatg gctctgaatt gacacttgat
    10741 cgctcaatga gcgctgggtt aactcgctac ttcccgacaa cagaactggc gcaaattggc
    10801 gatgaaactg cagacggcat ttatcatcca actgaattct ccccgctatc gcattttgat
    10861 gcgcgccgcg tcgatttttc cctcgcgcgg ttgcgccatt ataccggtac gccagttgaa
    10921 cattttcagc cgttcgtctt gtttaccaac tacacacgtt atgtggatga attcgttcgt
    10981 tggggatgca gccagatcct cgatcctgat agtccctaca ttgccctttc ttgtgctggc
    11041 gggaactgga tcaccgccga aaccgaagcg ccagaagaag ccatttccga ccttgcatgg
    11101 aaaaaacatc agatgccagc atggcattta attaccgccg atggtcaggg tattactctg
    11161 gtgaatattg gcgtgggacc gtcaaatgct aaaaccatct gcgatcatct ggcagtgcta
    11221 cgcccggatg tctggttgat gattggtcac tgtggcggat tacgtgaaag tcaggccatt
    11281 ggcgattatg tacttgcaca cgcttattta cgcgatgacc acgttcttga tgcggttctg
    11341 ccgcccgata ttcctattcc gagcattgct gaagtgcaac gtgcgcttta tgacgccacc
    11401 aagctggtga gcggcaggcc cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg
    11461 gtaaccacag atgacaggaa ctgggaatta cgttactcag cttctgcact tcgttttaac
    11521 ttaagccggg ccgtagcaat tgatatggaa agtgcaacca ttgccgcgca aggatatcgt
    11581 ttccgcgtgc catacgggac actactgtgt gtttcagata aaccgttgca tggcgagatt
    11641 aaacttcccg gccaggctaa ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt
    11701 ggcattcggg cgatcgattt gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta
    11761 cgaaccttta atgagccgcc gttccgataa taagttctga accttcttat cagaaataga
    11821 tgtgaaggag taagtaagac acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt
    11881 ccttgggcgg atacgtgttt tattatcgtc ttaat
//
LOCUS       DAFNBJ010000144        11565 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000144 DAFNBJ010000000
VERSION     DAFNBJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.144"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            144..1349
                     /gene="gltS"
                     /locus_tag="L2X49_003814"
     CDS             144..1349
                     /gene="gltS"
                     /locus_tag="L2X49_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBN4798827.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            1352..2218
                     /locus_tag="L2X49_003815"
     CDS             1352..2218
                     /locus_tag="L2X49_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798828.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETILAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(2203..4284)
                     /gene="recG"
                     /locus_tag="L2X49_003816"
     CDS             complement(2203..4284)
                     /gene="recG"
                     /locus_tag="L2X49_003816"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBN4798829.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELQLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(4290..4979)
                     /gene="trmH"
                     /locus_tag="L2X49_003817"
     CDS             complement(4290..4979)
                     /gene="trmH"
                     /locus_tag="L2X49_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBN4798830.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(4986..7094)
                     /gene="spoT"
                     /locus_tag="L2X49_003818"
     CDS             complement(4986..7094)
                     /gene="spoT"
                     /locus_tag="L2X49_003818"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBN4798831.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(7113..7388)
                     /gene="rpoZ"
                     /locus_tag="L2X49_003819"
     CDS             complement(7113..7388)
                     /gene="rpoZ"
                     /locus_tag="L2X49_003819"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBN4798832.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(7443..8066)
                     /gene="gmk"
                     /locus_tag="L2X49_003820"
     CDS             complement(7443..8066)
                     /gene="gmk"
                     /locus_tag="L2X49_003820"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBN4798833.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8206..8463
                     /locus_tag="L2X49_003821"
     CDS             8206..8463
                     /locus_tag="L2X49_003821"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798834.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            8485..10002
                     /gene="ligB"
                     /locus_tag="L2X49_003822"
     CDS             8485..10002
                     /gene="ligB"
                     /locus_tag="L2X49_003822"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBN4798835.1"
                     /translation="MEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTG
                     VRKMVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQK
                     VSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTL
                     NSLGVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELP
                     FVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVA
                     SLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTK
                     PTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEH
                     IFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASD
                     ERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(9999..10616)
                     /locus_tag="L2X49_003823"
     CDS             complement(9999..10616)
                     /locus_tag="L2X49_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBN4798836.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10907..11509)
                     /gene="dinD"
                     /locus_tag="L2X49_003824"
     CDS             complement(10907..11509)
                     /gene="dinD"
                     /locus_tag="L2X49_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBN4798837.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQANTTHFD
                     VGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
BASE COUNT         2555 a         2902 c         3185 g         2923 t
ORIGIN      
        1 aaaatccatg ccatttgttc attaacagct gagaatttga cccaggaaag cataactcga
       61 tacccgtcat agttcatgaa gttgccactg cgcgatgaag tatgacgagt atgaaagagt
      121 gatgcggata caaaggagta actatgtttc atctcgatac tttagcaacg cttgttgccg
      181 caacgctgac gttgctgctc gggcgtaagt tggtccattc cgtctccttt ttgaagaaat
      241 acaccatacc ggaacctgtt gcgggtggtt tgttggtggc gctggcgcta ctagtactga
      301 aaaaaagcat gggctgggaa gtcaactttg atatgtccct gcgcgatccg ttaatgctgg
      361 ctttcttcgc caccattggc ctgaacgcca acattgccag tttgcgtgcc ggtgggcgtg
      421 tggttggcat cttcttgatt gtggttgttg gtctgttggt gatgcaaaat gccattggca
      481 ttggtatggc tagcctgtta gggcttgatc cgctgatggg gctgttggcc ggttctatta
      541 cgctttccgg cggtcacggt acgggcgctg cgtggagtaa attgttcatt gaacgttatg
      601 gcttcaccaa tgcgacggaa gtggcgatgg cctgtgcaac gttcggtctg gtgctgggcg
      661 gcttgattgg cggtccggtg gcgcgctatc tggtgaaaca ctccaccacg ccgaacggta
      721 ttccggatga ccaggaagtc ccgacggcgt ttgaaaagcc ggatgtgggc cgcatgatca
      781 cctcgctggt gctgattgaa actatcgcgc tgattgctat ctgcctgacg gtggggaaaa
      841 ttgttgcgca acttttggct ggcactgctt ttgaactgcc gaccttcgtc tgtgtcctgt
      901 ttgttggcgt gattctgagc aacggtctgt caatgatggg cttttaccgc gtctttgagc
      961 gtgcggtatc cgtgctgggt aacgtaagcc tgtcgttgtt cctggcgatg gcgctgatgg
     1021 ggctgaaact gtgggagctg gcttcgctgg cgctgccgat gctggcgatt ctggtggtac
     1081 agaccatctt catggcgttg tatgccatct tcgttacctg gcgcatgatg ggcaaaaact
     1141 acgatgcggc agtgctggct gcgggtcact gtggttttgg cctcggtgca acgccaacgg
     1201 caatcgccaa catgcaggcg atcactgaac gctttggccc gtcgcacatg gcgtttttgg
     1261 tggtgccgat ggtcggtgcg ttctttatcg atatcgtcaa tgcgctggtg attaagctgt
     1321 atttaatgtt gccgattttt gccggttaaa aatgaaaaag cgtcggaagg aaccggaaac
     1381 gctacgtgaa cactgtcgcc atatttttgg tgatgagccg ccagtgctct gtgtatggga
     1441 aacggaattt gattacgcag atgccgaact caaggcgctg gcggctaaag agtggcagca
     1501 gatcagcgaa tgggatttga gtgcttacta tgtactcaat ctcgtttata acgaaccgat
     1561 gcaaatagag ttgtttcgct atctgtttcc gctctgcctg gcgcaatggc atgaaaccat
     1621 tttagctggc ggttatggtg atcacttcga agaatcctta atgaaggcat tgtgtcggcc
     1681 ctatctctgg caggagatga tgaatgcttc ccaacgccag caggtacggc aatttttact
     1741 ggacaccgcg ttgcagcgta tggataacga gcgtggtttc aataacgttc tttgctggct
     1801 ggcggtgttc aatactctgg gtggggccgc gccgttaatt cgttctctct ggtcgcgctg
     1861 gtgggcgctg gatacgccag gaaaagcggt gtgtgctatt cagtatgccg cgcatctaat
     1921 ttatcccata gaagccaatc cgttatggtc gcaagaatgg ataggctggg ggcatccatt
     1981 aggccataaa gatggctggt cctctgataa tcgcgctttt cttcgccaaa tgttaacacc
     2041 tgaaatgatt gtggctggcg tacaggcagc ggctgaaatt ctgcgtggtg aaccggaagg
     2101 cgcaatggcg gcgcgaattg cacaggatgc gtatgaggca atggatatcc tcactatcca
     2161 gattgaagat ttactgcgtg acttgtcttg tgatgagtct ggtcatgcgt tggagtaacg
     2221 ttccgtctcc ggcatccagc gttctatcag ggcttttgct tgttgtgggt aacgttcgtg
     2281 aatatgtcgc gccaggcgct gaacttccgg gatcatcgcc tgatcgcgca gtaaatccgc
     2341 cactttaaat tcagcattac ccgtctgacg cgtgcctaac aattcgccag ggccgcgaat
     2401 ctccagatct ttttgcgcaa tcacaaaacc gtcgttactg tcgcgcagca cttgcaggcg
     2461 aatttgcgcc gttttggaga gcggcgtttt gtagagcagc acgcagtgag aagccaccgc
     2521 gccacgacct acgcgcccgc gcagctggtg taactgcgct agacccagac gctcagggtt
     2581 ttcgataatc atcagactgg cgttaggcac atcaacgccg acttcaataa cggtagtggc
     2641 aaccagcagt tgtagctcac cttgtttaaa cgacgccatc accgcctgtt tctcggctgg
     2701 tttcatccgc ccgtgtacca ggccaacatt caattctggt agcgccagtt tcaactcttc
     2761 ccaggtagct tccgccgcct gcgcttccag taactctgac tcttcaatca acgtacaaac
     2821 ccagtacgcc tgacgacctt cagtcatgca ggcgtggcgc acgcggtcaa tgatgtcggt
     2881 acggcgggta tcaggaatag cgaccgtagt cactggcgtg cggcctggcg gcagctcatc
     2941 tatcaccgag gtatcgagat cggcatacgc cgtcattgcc agcgtgcggg ggatcggcgt
     3001 ggcggtcatg atcaactgat gcggatggaa gccctgctgc tggcctttct cccacaatgc
     3061 cagacgctga tgcacgccaa aacgatgctg ttcgtcgata atcaccagcg ccagaccgtt
     3121 aaactgcacc tgttcctgga agatggcgtg tgtaccgaca atcatctgca cctgaccgct
     3181 ggcgatggct tcctgctgtg ccagtcgcgc tttacctttc tgcttaccgg cgagccagcc
     3241 cacttcgata ccgagcggtg caaaccagtt gcggaagtta ttggcgtgct gctcggcgag
     3301 taattcggtt ggtgccatca gggccacctg tttgccgtgg gcaatcgcac gcagcgcggc
     3361 gagggcggcg accagtgttt taccggaacc tacatcgccc tgcaccaggc gcatcattgg
     3421 aacatccagc gccatatcat gctcgatctc cgccactacg cgtgcctgtg cgcccgttgg
     3481 cttgaacggt aaggcggcga ggagtttatt tttcagcgcg tcattggcgc tcagcggctg
     3541 ggcatgaaaa cgctgtgctc cggcacgtaa ggctaacatg ctgaggttgt gcgccagcag
     3601 ttcttccaga atcagacgac gttgcgccgg atgctgcccg gtttccagat cgctaagctg
     3661 tagcgtcggc ggcgggcggt gcaaagtgcg caacgcttcc ggtagcgtca tcattccttg
     3721 tgacagttcc ggcggcagga gttcttcaat ggcgcaggtg tcgagcagat ccagcgcctg
     3781 gtcggttaat ttacgcagcg tggcctgctt tacgccttcc gttgttggat aaaccggcgt
     3841 gagcgtttcc tgtaattctg gcgtgctgag atcgccctgc acgcggtatt ccgggtggat
     3901 catctccgca ccatatttac cgcgctttgc ttcgccataa gccagtacac ggcggcccgt
     3961 cgccaggcta tttttcattg ccgcgctgaa attgaaaaag cgcatggtga ggatgccgga
     4021 accgtcgctg atctggcagg tcatcatccg ccgaccgccg aaggagatat tgcagttcag
     4081 cacttcgcct tccaccgtgg cataaacgcc cggcagtagt tctccgatgg ggtagagatg
     4141 ggtgcgatct tcgtagcgca ggggaaggtg taagagtaga tcctgcacgg tatgcaggtt
     4201 gattttcgcc agtttgttac taagtgctgc gccaacgccc gttagggaac tgagtgggac
     4261 agcatctaac aggcgacctt tcatggcact taccctgcag cctgcatagt agcccaccag
     4321 tcggcatcag cttcgatctc gccttgctga ttgacgtggg gataaggcag gcctttgcgt
     4381 tttgcgactt tcgccagcac cggatagccg ccttcaaaca acaggcgttg ttgctctgct
     4441 tccggcaaca tgctgttttc acgcaggtac atgcctgcat tttgccgctg acgctgggct
     4501 tcgtaaagaa tgagggctga ggcaacggaa acattcagcg actgcaccat gccgatcatc
     4561 ggaatgatga tgtcctgatc cgccagggcc aatgcttcct gcgtgatgcc cgttttctcc
     4621 tgtcccatca aaatgcaggt cgggcgagtg taatcaattt cgcggaaatc gacagcgtta
     4681 tcagaaagat gggttgccag aatctgcatg ccctggcctt tgagatgagc gacggcatcg
     4741 ccaatggtgc ggtgtgtttt cacctgtacc cagctgttac tacccgccgc tgccgaagcc
     4801 atggtgcgca tgcggctacc aggccagacg gcgtgaactt catgtacgcc aacggcatct
     4861 gcggtacgaa taatcgcaga aacgttatga ggtttgtgga cctgctccat gcagacggtc
     4921 agatcaggct gccgcctggc gagcatttcg cagatgcgtg cataacgtgt tgggttcata
     4981 aaacattaat ttcggtttcg ggtgacttta atcacgtctg gcatcacgcg gattttgcgc
     5041 atgatattcg ccagatgcac acggtcacgg gcggtcagac gaataaaggc gctgtagacg
     5101 cgaccatctt tctcttccgt attcaaactt tgaatattcg aagtcgtggt gttaattgcc
     5161 gccgtcaggt ttgccagcgc gccctgatga ttgaacatct ccaccttgat ttcggtgatg
     5221 aactcctgcg ccgtctcttt atcccattcc acagccataa acttctctgg ctctttctgg
     5281 tagccacgga tattacggca ggattcatgg tggatcacca gacctttacc ggggctgacg
     5341 tgggcgataa tcgggtcgcc aggaataggg cggcagcatt tcgcaaaggt gatcagcacg
     5401 ccatcggcac ctttaatggg cagatgtccg tggctttggg ttgccggtgg aatggaggcg
     5461 tccccatgtt gcagattttt cgcgaccacc acgctcattg cgttaccaag tccgatttct
     5521 gccagcagat cgtcaagcgt tgccagcttc atgcgatcca gctcgcgctg aatattttcc
     5581 tgcgggattt cattcagctt acggctacca cccaaagcat ggttgagcag acgacggccc
     5641 aggcttacag aatcatcacg cttgaggttt ttcagcaact gacgaatttt ggcgcgcgct
     5701 tttgagctaa cgacaaagtt cagccacgcg gcattcgggc gagcgcccgg tgcggtaatg
     5761 atttcaacgg tttgaccgct ggtaagcggc tgcgacagcg ggtaaggctg gcggtcaacg
     5821 cgtgcgccca cgcaggcatg accgatatcg gtatgcactg cataagcgaa gtcgacgggc
     5881 gttgcaccgg caggcagctc gacaattcgc ccttccggtg tgaaaacgta aatctcatcc
     5941 gggaagagat cggatttaac gctctcgata aattcaaacg aactaccggc gctctgttgc
     6001 agctccagca ggctttgcat ccagcgctgg gcgcggattt gcgcggtggt gctggtttcg
     6061 ccgtgctctt tataagccca gtgcgcggca acacccatct ccgccatctg atccatatct
     6121 tcggtacgga tctggacctc aaccggcaca ccgtgcgggc cgatcatcga ggtgtgcaaa
     6181 gactgatagc cgttcgcttt tggaatggcg atgtagtctt tcacgcgacc cggacgcggc
     6241 ttgtacaggc tatgcatttg cccgagcacg cgataacagg tgtcagaatc attgacgatc
     6301 acgcggaaag cgtagatgtc catgattgag tgaaaacgct gctctttgag caccattttg
     6361 cagtaaatcg aataaagatg cttttcgcga ccactgacgc ggcacggtat tcccgcttcc
     6421 tgcaaacgcc cttcgatttc agagaggatt ttttggatca tctctttacg gttgccgcgc
     6481 gcggctttca ccacttcttt aattacgcgg taacgattgg gatacagcgc ctcaaaaccc
     6541 agctcttcga gttcggtttt aatgtggtgg atacctaaac ggtgcgccag cgggctataa
     6601 atttcgagag tttcacgggc gatgcggcga cgtttgtccg ggcgaagtga gcccagcgtg
     6661 cgcatgttgt gggtacggtc ggcaagtttg atgaggatga cgcggatatc ctgcaccatc
     6721 gccataatca tcttgcgaaa gttttcggcc tgcgcctctt tcttatcgcg gaacttgagt
     6781 ttatcaagtt tcgacacccc ctctaccagc tcggcgacgc ttttaccaaa aagctgttcc
     6841 atatcctggt aggtggcggg agtatcttca atcacgtcat gcagcagcgc cgccatcagc
     6901 gtttcatagt cgagtttcat ctcggccaga atgcaggcaa ccgctaccgg gtgcgtgata
     6961 tagggttcac cgcttgaacg tgtttgcccc tcgtgagcat cacgtgcaac gagatacgcc
     7021 tgccgcagac gcttgatttg gtcttccggc aggtaggttt gaatcagttg attcaggctt
     7081 tcaaacagat acaagggcga cccgctttgt gattaacgac gaccttcagc aatagcggta
     7141 acggcttgta attcagcggc ttcctgctct tgctgttcct ggcgttcgcg aacgtcgagg
     7201 atctggttgt tgatcagacc ttcttcgatt tcgcgcagcg cgattacagt ggttttatcg
     7261 ttttcttccg gtaccagcgg atcctttccg cctacctgca tctgacgagc gcgacgcgcg
     7321 gcgaccagta ccaggtcaaa acggttacca attttctcta cagcgtcctg aacagttacg
     7381 cgtgccatac ttaaaaagct ccacaggtga agaaatgact gggcatgata ctgaaatcag
     7441 gttcagtctg ccaacaattt gctgattaaa gcgtcatgac gctgcttttg gcggctcatg
     7501 cgcagacgtt cggcgcgaat aatggtcttc aaatcggtca acgcggtatc gaagtcatca
     7561 ttcacaatca gataatcata ttcggcgtaa tggctcattt ctgcaacagc ttgcgccata
     7621 cgctttgcaa tgacctcttc gctgtcctga ccgcgaccgc gtagacggcg gtccagttca
     7681 attttggacg gcggtaaaat aaagatactc cgcgcgtgcg gcatcttctg gcgaatttgc
     7741 tgcgcaccct gccagtcgat atcgagaaaa acatcgacac cggtcgccag tacttgctca
     7801 atggcctcac gcgaagtgcc atagtaatta ccaaaaactt ctgcgtgttc gaggaacgca
     7861 tctctgctaa tcatttcttt aaattcatca tgattaacaa agaaataatg ttcaccgtgg
     7921 acttcaccag gacgcggttg gcgtgtggtg tgtgaaacag aaacctgggt gtcatacaac
     7981 ggttgggttt ttaataaagc ctgaatcagg ctggatttac ccgcgccact gggggcagaa
     8041 acaatataaa gcgtgccttg agccatgagt atctttcgta tgtgattagc gaaataaagc
     8101 ctacatacga gcttattata cacggcgcgg ccatgtgacg tagcccttgc tcacactttt
     8161 tgcgcaaagt tgttgcgttt tgcgtagctg tttccgcatt tgcctgtgat ttactgcaat
     8221 aaagacgatt ctcaggaagc gtcctcgcag actccgtttt tgccactcgg ctttgccttc
     8281 accacgttgt atatctgcca gcctgactac agcggaggtg aagatgaaag tatggatggc
     8341 gatattaata agtatcttgt gctggcaatc atctgtgtgg gcggtctgtc cggcctggtc
     8401 gccagccaga gcacaggaag aaatttcccg cctgcaacag caaataaaac agtgggacga
     8461 tgactactgg aaggaaaaag tgaggtggaa gacggtgttt acgatcagtt aagcgcccgt
     8521 cttacgcagt ggcaacgctg ctttgggagc gagccccgcg acgtgatgat gccgccttta
     8581 aatggtgcgg ttatgcatcc ggttgcccat acgggtgtac gcaaaatggt ggataaaaac
     8641 gcattaagtc tgtggatgcg agaacgtagc gatctttggg tgcagccaaa agttgatggc
     8701 gttgcggtaa ccctggttta tcgggacggg aaactgaaca aagcaatcag tcgcggtaat
     8761 ggcctgaaag gcgaggactg gacgcagaaa gttagcttaa tttccgctgt gccgcaaacc
     8821 gttagcgggc ctttagccaa cagtacgctt cagggggaaa tatttctcca gcgcgagggg
     8881 catatccaac aacaaatggg gggaataaat gcccgcgcaa aagttgctgg cttgatgatg
     8941 cgccaggacg atagcgacac gctgaattct ctgggcgttt ttgtctgggc atggccggat
     9001 ggaccgcagt taatgtctga tcgtttaaaa gagctggcta ccgcaggttt tactctaacg
     9061 cagacgtata cccgtgcggt gaaaaatgct gatgaagttg cgcgcgtacg caatgagtgg
     9121 tggaaagcgg aattaccctt cgtcaccgat ggcgtagttg tgcgaggggc gaaagaaccc
     9181 gaatcccgcc attggctacc aggccaggca gaatggctgg tggcctggaa atatcaacct
     9241 gtagctcagg ttgccgaagt gaaggcgatt cagtttgcgg tgggtaagag cggtaaaata
     9301 tcggtggttg cgtcactcgc acctgtcatg ctggatgata aaaaagtcca gcgggtgaat
     9361 attggttccg tcaggcgctg gcaggagtgg gatattgcgc ctggtgatca gatcctcgtc
     9421 agccttgccg gtcagggtat tcctcgcatt gatgatgtgg tctggcgcgg tgcagaacgt
     9481 acaaaaccga caccaccaga aaaccgcttt aactcgttga cctgctactt tgcttctgat
     9541 gtttgtcagg aacagttcat ttcacgctta gtctggctgg ggtcaaaaca ggttctgggg
     9601 ctggatggca ttggtgaggc cggctggcgc gcgctgcatc agactcatcg ctttgagcat
     9661 atcttttcct ggcttttatt aacgccagag caattacaga acacgccggg gatcgcgaaa
     9721 agtaaaagtg cgcagctatg gcatcagttt aatctggctc gtaagcagcc ttttactcgc
     9781 tgggtgatgg caatgggaat accgctaacc cgggcggcgc ttaatgccag tgatgaacgg
     9841 tcctggtcgc aacttttatt tagcacggag cagttctggc agcagctgcc ggggactgga
     9901 tcggggcgcg ccagacaggt tattgaatgg aaggaaaatg cgcaaatcaa gaagctgggc
     9961 agttggctgg ctgcccagca gatcacaggt tttgaacctt agtgtccttc gtggctgtag
    10021 taaaagaccg gtaatcccag cttaaggcgt agcgccagta agcgggcaaa aaagccgaat
    10081 accagggtgg agataattac cacatcatgg ttagaaacat agtgttgcag cgcgatgtac
    10141 agcacggcgg aggcaaatga gacaccggca tacaactctt tctggaatac cagtgggata
    10201 cgtttacaga acatatcgcg caaaacgccg ccaaacacgc cggtggttac cgccgcgaca
    10261 acggcaataa ttgggccgtg ccccatatcc agtgcaacct gtgcgccgat gatagaaaag
    10321 accaccagtc caagcgcatc gagcaccaga aacactttgc gcaggtaagg cattacagga
    10381 gcaacgatag tggtgagtac tgcggcggta gcgacgataa tcacatattc cgggtgtttg
    10441 acccagccga gcggatagtg gcccagcaga atatcgcgca ctgaccctcc gccaattgcg
    10501 gtggcggtcg caataataat tacgccaaat gtatccatgc ggcgtcgccc ggccgccaga
    10561 gcccccgtca tggcttcggc agtaatgcca accaaataca aaatgtgtaa cagcatggtt
    10621 ctctccctaa aaatctgtct ggcagggtag cgatttgtgc gtattgtcac gattgagatt
    10681 ttttaaggtg agaggaaatt gataacttgt agttatgtga aatcgctatt ttctgtagcg
    10741 tagatgcatt cttctgactt cttgtctgag aaaatgcata agaaaaaata ggttaaaact
    10801 gaggggggaa aagaaaggtg caatgtttag ccattgtgtt cactgcacct ggaccttggc
    10861 ttacgcatcg ggtgtctcaa tcaacaaaaa gagtttttcg tttctgctat ttcttctctg
    10921 taatttttac actgttttcc aactgcttga tgctgacctg cggggtcggc aactcctcag
    10981 gcatggttcc gccaagttcc tgaatggttt gcctcacctt gctacccacg tcaaagtgtg
    11041 tggtattagc ttgctgtgct gcctcaacca attgtttgtt atgttctttc aactcatttc
    11101 tcagaaacag acgcttttcg tcttcacgaa gttgcttaaa tgcttcatcg tcagcaagct
    11161 cctgccgtcg ggtctggata gcaaaataag tctgccctgc cgcaatgacc ggtttcgcgg
    11221 ggtcgccgtt ttgtactacc aggtaacagg catagcggga gagtacgatg tcttttaatg
    11281 ctcttttggc atttgagcca attttgacca tatcgaggat ctcctcgaaa tggtcatcaa
    11341 ttgtgtgacc actgttctca caggcctctt tggcgcgcgt taatacaggt ataaagtgac
    11401 gatactctga ataacccagt agtttcccaa gttgtcttgc tgaccattgt tctgctccgt
    11461 ttgcattaat cagttttatc tcttcaaaag gttgatgatg ttcgttcatt gcccactcca
    11521 tgtgatatcg attgcaaaga aagtaaatct gtaactgtat ttata
//
LOCUS       DAFNBJ010000145        11546 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000145 DAFNBJ010000000
VERSION     DAFNBJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.145"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            58..2484
                     /locus_tag="L2X49_003825"
     CDS             58..2484
                     /locus_tag="L2X49_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HBN4798838.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            2545..4968
                     /locus_tag="L2X49_003826"
     CDS             2545..4968
                     /locus_tag="L2X49_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HBN4798839.1"
                     /translation="MKIHTTEALIKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGKEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4979..5596
                     /gene="dmsB"
                     /locus_tag="L2X49_003827"
     CDS             4979..5596
                     /gene="dmsB"
                     /locus_tag="L2X49_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBN4798840.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5598..6452
                     /locus_tag="L2X49_003828"
     CDS             5598..6452
                     /locus_tag="L2X49_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBN4798841.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            6495..7109
                     /gene="dmsD"
                     /locus_tag="L2X49_003829"
     CDS             6495..7109
                     /gene="dmsD"
                     /locus_tag="L2X49_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBN4798842.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7268..8560
                     /gene="clcB"
                     /locus_tag="L2X49_003830"
     CDS             7268..8560
                     /gene="clcB"
                     /locus_tag="L2X49_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBN4798843.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFHHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYELIISTGVLAGLCGPLLLTLMNACHRGFVGLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(8513..9208)
                     /gene="bioD"
                     /locus_tag="L2X49_003831"
     CDS             complement(8513..9208)
                     /gene="bioD"
                     /locus_tag="L2X49_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBN4798844.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9333..10553)
                     /gene="mlc"
                     /locus_tag="L2X49_003832"
                     /gene_synonym="dgsA"
     CDS             complement(9333..10553)
                     /gene="mlc"
                     /locus_tag="L2X49_003832"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBN4798845.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10688..>11546)
                     /locus_tag="L2X49_003833"
     CDS             complement(10688..>11546)
                     /locus_tag="L2X49_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4798846.1"
                     /translation="AVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRS
                     VLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLS
                     LFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMI
                     PHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAM
                     TIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNF
                     RIHLLNALR"
BASE COUNT         2805 a         2985 c         2960 g         2796 t
ORIGIN      
        1 gcgctaaata ttcaatatat aaacatttat ataacgataa agaacaggga gtgagttatg
       61 tccaaaaatg accgaatggt gggcatcagc cgcagaacac tcgttaaatc aaccgcgata
      121 ggttctctgg cgctggctgc aggcggtttt tctttaccgt tcaccctgcg cagtgcagcg
      181 gcaacggtac aacaggccag cgaaaaagtg atctggggtg cctgttccgt caactgtggt
      241 agccgctgtg cacttcgtct acatgttaaa gataatgaag tgacctgggt ggaaactgac
      301 aataccggca gcgatgagta cggcaaccat caggtacgcg cctgtttgcg cggtcgctcc
      361 atccgccggc gtattaatca tcccgatcgc ttgaattacc caatgaaacg cgtgggtaca
      421 cgcggtgaag gcaaattcga acggattagc tgggatgaag ccctggatac tatcgccagt
      481 agcctgaaaa aaaccgtcga acaatatggc aatgaggctg tatatattca gtactcttcg
      541 gggattgttg gcggcaatat gacccgctct tcgccatcag cctcggcggt caaacgcctg
      601 atgaactgct acggcggttc actcaatcag tatggctcct acagcaccgc gcaaatttcc
      661 tgtgccatgc cctacaccta cggcagtaat gatggcaaca gcaccacgga tattgaaaac
      721 agcaagctgg tcgtgatgtt tggtaacaac ccggcagaaa cccgcatgag cggcggtggc
      781 atcacttatc ttcttgaaaa agcgcgcgag aaatcgaacg ccaaaatgat tgttatcgat
      841 ccgcgatata ccgatacggc tgctggtcgt gaagatgaat ggctccctat tcgcccgggc
      901 accgatgccg cgctggttgc gggtattgcc tgggtattga ttaacgaaaa tctcgttgat
      961 caaccttttc tcgataaata ctgcgtcggc tatgacgaaa aaaccttacc cgcagatgca
     1021 cccaaaaatg gtcactataa agcctatatt cttggtgaag gtgatgataa cacagcgaaa
     1081 acgccgcagt gggcttcgca aattaccggt atcccggtag accgtatcat caaactggcg
     1141 cgtgaaattg gcacagcaaa acccgcttat atctgccagg gctgggggcc gcaacgccag
     1201 gcaaacggcg aactgactgc acgcgctatt gctatgttac ctattttgac gggcaatgtc
     1261 ggcatcagcg gcggaaatag tggcgcgcgt gaatcgacct ataccattac catagaacgc
     1321 ctgccggtgc tggataatcc ggtcaaaacg tcaatctcct gcttcagctg gacagatgct
     1381 atcgatcatg gtccgcaaat gacggcaatc cgcgacggcg tccgcggcaa agataaactg
     1441 gatgtgccca ttaagttcat ctggaactac gcgggaaata ccctcgttaa tcagcattct
     1501 gacatcaaca aaacgcatga aattctgcaa gacgaatcga aatgcgaaat gattgtggtc
     1561 atcgaaaact ttatgacctc atcggcaaaa tatgccgaca ttctgctgcc agacctgatg
     1621 accgttgagc aggaagatat tattcctaac gactacgccg gtaacatggg atatctcatt
     1681 ttcctccagc cagtcaccag cgaaaaattc gaacgtaaac cgatttactg gatcctgagt
     1741 gaagtcgcga aacgtcttgg acctgacgtc tatcaaaagt tcactgaagg tcgcacgcag
     1801 gaacaatggt tacaacatct gtacgccaaa atgcttgcca aagatccggc gttaccgtct
     1861 tacgacgaac tgaaaaaaat gggtatctat aagcgtaaag atcccaatgg tcattttgtc
     1921 gcctacaaag catttcgtga cgaccccgag gcaaatccac ttaaaacacc ttccggtaag
     1981 attgaaattt attccagcaa gctggcggaa attgcccgta cctgggaact ggaaaaagat
     2041 gaagtgataa gcccactgcc agtttatgcc tctacctttg aaggctggga ctcccctgaa
     2101 cgtagaacct tcccacttca actgtttggt ttccattaca aatcccgtac tcactcgacc
     2161 tacggcaata ttgatctcct gaaggctgcc tgccgtcagg aggtgtggat caaccctata
     2221 gatgcgcaga aacgtgggat tgccaacggc gatatggtgc gggtgtttaa ccatcgtggc
     2281 gaagttcggc taccagccaa agtgacacca cgcattctcc ctggggttag cgctatgggc
     2341 cagggagcct ggcacgaggc caatatgtct ggcgataaaa tcgaccatgg cggctgtgtg
     2401 aatacgctaa ccactctgcg cccttcacca ctggcgaagg gaaacccgca gcacactaat
     2461 ctggtcgaga tcgaaaaaat ataacccacg acaaccataa attctggcat gacattttgt
     2521 ttgaaaagca ataagtgagt aatgatgaaa atccatacca cagaggcgct cataaaggct
     2581 gaaatcagcc gtagaagtct gatgaaaacc tccgcacttg gcagtcttgc gctggcaagc
     2641 agtgctttca ctctgccatt ttctcaaatg gtccgggcgg cagaggctcc ggtagaagag
     2701 aaagcggtct ggagttcctg caccgttaac tgcgggagcc gctgtctgtt acgtttgcat
     2761 gtgaaagatg acaccgtgta ctgggtggag tctgatacga caggtgacga cgtctacggt
     2821 aatcatcagg ttcgcgcgtg tttacgcggg cgctctatcc gccgacggat gaatcatcct
     2881 gataggttga aatatcccat gaagcgcgtc ggcaagcgcg gtgaaggtaa atttgaacgg
     2941 ataagttggg acgaagccct ggataccatc agtgataatc ttcggcggat cctaaaagat
     3001 tacggcaatg aggctgtaca tgtcctgtac ggaacaggcg tagatggcgg aaacatcacc
     3061 aactcaaacg tcccgtaccg tctgatgaac tcttgcggtg gttttctcag tcgctatggc
     3121 agctacagta ccgcacagat cagtgccgca atgagttata tgttcggtgc caatgatggc
     3181 aacagcccgg atgatatcgc caatacgaaa ctggtcgtta tgttcggaaa taacccggca
     3241 gaaacgcgga tgagcggcgg tggtgtcact tactacgtcg agcaagcccg cgaacgttca
     3301 aacgcacgca tgatcgtcat cgatccacgt tataacgaca cagctgccgg gcgtgaagat
     3361 gaatggctgc ccattcgccc aggcaccgat ggcgcactgg cctgtgcgat tgcctgggta
     3421 ctgattactg aaaacatggt cgatcagcca tttctcgaca aatattgtgt tggttacgat
     3481 gaaaaaactc tgcccgccaa cgcaccacgt aacgcgcatt ataaagccta tattctgggc
     3541 gaagggcctg acggcatagc taaaacgccg gaatgggcag caaaaatcac cagcatcccg
     3601 gcagaaaaaa ttatccagtt ggcacgagag atcggttcag caaaacctgc ttatatttgt
     3661 cagggttggg ggccacaacg acattccaac ggcgagcaaa cgtcccgcgc tattgccatg
     3721 ctttccgttc tcaccggtaa cgtcggcata aacggcggca actcaggtgt acgcgaaggt
     3781 agctgggatc tgggggtaga atggttcccg atgcttgaga accctgttaa aacgcagatt
     3841 tccgtcttta catggacaga tgccatcgac catggtaagg aaatgaccgc gacccgtgat
     3901 ggtgttcgtg gaaaagaaaa actggatgtc cccatcaagt ttttatggtg ctacgccagt
     3961 aacacattga tcaatcaaca tggcgacatc aatcacaccc atgaggtgct tcaggatgac
     4021 agcaagtgcg agatgattgt tggcattgac cacttcatga cggcctcggc taagtattgc
     4081 gatatcctgt tgcccgacct gatgccaaca gagcaagaag accttatctc tcatgaatct
     4141 gcagggaata tgggctatgt gatcctcgcc caacccgcaa cctctgcaaa atttgaacgc
     4201 aaacccatct actggatgct gagtgaagtc gccaaacgct taggaccaga cgtttatcaa
     4261 acctttactg aaggtcgcag tcagcatgaa tggatcaaat atctccatgc gaaaacgaag
     4321 gaacgtaacc ctgagatgcc cgactacgag gagatgaaaa cgaccgggat ctttaagaaa
     4381 aaatgcccgg aagaacacta cgtcgctttc cgcgcattcc gtgaagatcc ccaggcaaac
     4441 ccgttgaaaa caccttcggg gaaaatcgaa atttattctg aacgactggc gaagattgcg
     4501 gatacctggg aattgaaaaa agatgaaatt attcatcccc ttccagctta tacgccaggt
     4561 tttgatggct gggacgatcc cctgcggaaa acctatccac tgcagttaac gggcttccac
     4621 tacaaggcgc gtacccactc cagctacggc aatattgatg tgttacagca ggcctgccca
     4681 caagaggtgt ggatcaaccc cattgatgct caggctcgcg gtatccgtca tggcgatacc
     4741 gtgcgggtat ttaacaataa tggagaaatg ctgattgccg caaaagtgac tccgcgtatt
     4801 ctgcctggcg ttaccgccat cgggcaaggt gcgtggctta aagcggatat gtttggtgac
     4861 cgggtcgatc acggcggcag tatcaatatt ctgacctctc accgcccttc accgctggca
     4921 aagggaaacc cgtcgcacag caatcttgtc cagatcgaaa aggtttaaag agtagcccat
     4981 gaccacacaa tatggatttt ttatcgattc cagccgctgt actggctgta aaacctgcga
     5041 actggcgtgc aaagatttta aagatcttgg cccggaagtc agtttccgcc gtatttatga
     5101 atatgctggc ggcgactggc aggaagataa tggcgtctgg caccagaacg tgttcgctta
     5161 ttatctctcc atctcctgta accattgcga tgacccagca tgtacaaaag tctgccccag
     5221 cggcgcaatg cataagcgtg aagatggctt tgtggtggtt gatgaagatg tttgtatcgg
     5281 ctgccgctac tgccacatgg cttgtccgta cggcgcacca cagtacaatg ctgaaaaagg
     5341 gcacatgacg aagtgcgatg gttgttattc gcgcgtcgcc gaggggaaac aacccatatg
     5401 cgtcgaatcc tgcccgctgc gtgcacttga gttcggtcca attgaagagc tgcgccagaa
     5461 gcacggcact ctggctgccg tcgcgccact gccgcgcgcg catttcacaa aacccaatat
     5521 cgttatcaaa cctaacgcca acagccgccc gaccggcgat accactggtt atctggctaa
     5581 tccggaggaa gtgtaaaatg ggaaatggat ggcatgaatg gccactggtt atctttaccg
     5641 tattgggtca atgcgtagta ggcgcgctga ttgttagcgg aattggctgg ttcgccgcca
     5701 aaaatgacgc agaccgtcag cgtattgtcc gtggcatgtt tttcctctgg ctgttgatgg
     5761 gcatcgggtt tattgcctct gtcatgcacc tgggatcgcc actgcgagcg tttaactcgc
     5821 tgaatcgaat cggagcttct ggtctgagta acgagattgc tgccggttcc atattttttg
     5881 ccgttggcgg actatggtgg ctggtggcgg tcatcggtaa aatgccgcaa gcgttgggga
     5941 aactctggtt acttgtcagc atggcgctcg gcgtcatttt cgtctggatg atgacctgcg
     6001 tgtatcaaat cgacaccgtg ccaacctggc ataacggtta cactacgctg gctttcttcc
     6061 tgacggtatt gttgagcggt ccaatacttg ctgccgcaat tctgcgggca gctcgcgtta
     6121 catttaatac cacaccattt gccatcatta gcgttctggc gctgatcgct tgtgccgggg
     6181 tgattgtgct gcaaggtttg tctctggcgt gtattcactc atccgtacaa caagccagcg
     6241 cactggtacc agattacgcc tccttacagg tctggcgtgt ggtattgtta tgcgcaggtc
     6301 taggatgctg ggtttgtccg ctcattcgtc gccgtgaacc tcacgttgcc ggacttattc
     6361 tggggttaat cctcattctt ggcggtgaga tgatcgggcg tgtgctcttt tatggcctgc
     6421 acatgacggt aggaatggct attgcaggtt aacaaattgg tgcggggcaa cccgcacatt
     6481 tcaggatgtt aggaatgacc catttttcac agcaagataa tttttctgtc gcggcgcgcg
     6541 tgttgggcgc gctgttttat tacgctccag agagtgcgga agccgcacct ctggttgcgg
     6601 tactcaccag tgacggctgg gaaactcagt ggcctctacc agaagcgtca ttagcgccgc
     6661 tggtgacggc ttttcagaca cagtgtgaag agactcacgc ccaggcctgg cagcgtttgt
     6721 ttgtcggccc gtgggcactg ccgtctccgc cgtgggggtc ggtctggctg gatcgcgaat
     6781 ctgtgctgtt tggcgattca acattggcac ttcgtcagtg gatgcgcgag aaaggcattc
     6841 agtttgaaat gaagcaaaac gaaccggaag atcattttgg ttcactgctg ttgatggctg
     6901 cgtggttggc ggagaatggt cgccagacag aatgcgaaga attgctggca tggcaccttt
     6961 ttccgtggtc aacacgtttt cttgatgttt ttatcgaaaa agcagaacac cctttctacc
     7021 gtgcactggg tgaactggct cgcttaacgc tggcgcaatg gcaatcacaa ctgttgattc
     7081 ctgtcgcggt taaaccgctg tttcgatagg atcaccgtaa tattgccgga tggtgacgat
     7141 tacgcgcaat ccggcaataa taggttacag tgtcacgttt ttttatctct taaagcacgc
     7201 actgcttttg cggctggcct cttttgtcgc aaaatagtcg cccgtgtttc attgcccatt
     7261 tctgctcatg catcatctac acatctatcc ggatctgcgc actatgttcc gccgtctgct
     7321 tatcgcaaca gtcgtcggta ttctcgcggc ctttgccgtt gccgggtttc atcatgcgat
     7381 gctgctactg gagtggttgt tcctcaataa tgactccggc agtctggtca atgcagcgac
     7441 aaacctttcc ccctggcgac ggctgctaac tccggcgctc ggcggactgg cggcgggttt
     7501 gttgctgatg ggctggcaga aatttaccca acaacgccct catgcgccga ccgattacat
     7561 ggaagcgttg caaaccgatg gacagttcga ttacgcagca agcctggtta aatcgcttgc
     7621 ctcgctgctg gtagtaacca gcggcagtgc aattggtcgc gaaggtgcga tgattctttt
     7681 agctgccctt gccgcctcct gttttgccca acgttttacg ccacgccagg agtggaaatt
     7741 atggatcgcc tgtggggccg cggcgggaat ggctgcggcc tatcgtgccc cgcttgctgg
     7801 cagtttattt atagccgaag tgctgtttgg cactatgatg ttggcctctc tcggcccggt
     7861 gattatttcc gccgtcgtgg cattgctggt tagcaatctg attaatcata gcgacgcgtt
     7921 actctacaac gtacaactct cagtgacggt tcaggctcgt gactatgagc tgattatcag
     7981 tacaggtgtg ctggcaggtc tgtgcggacc actgttgtta acgttaatga acgcctgtca
     8041 tcgtggattt gtgggtctca aacttgcgcc gccctggcaa ctggcactag gcgggttgat
     8101 cgtgggtctg ctttccctgt tcacacctgc agtgtggggc aacggctata gcaccgtaca
     8161 atccttttta accgccccgc cactgttaat gatcattgcc gggatcttcc tttgtaaact
     8221 gtgtgccgtg ctggcgagta gcggttccgg cgcacccggt ggtgtcttta caccgacgct
     8281 atttatcggt cttgccattg gcatgttgta tggtcgtagc ctgggattat ggttccctga
     8341 tggcgaagaa attacgcttt tactcggatt gaccgggatg gcgacactgt tggcggcaac
     8401 cacgcacgcg ccgattatgt cgacgttgat gatatgtgaa atgaccgggg agtatcagct
     8461 actccccggt ttattgattg cctgcgtaat tgcgtcggta atctcgcgga cgttacaccg
     8521 tgactctatc taccgccagc acactgcgca gcatagctaa gcggatgtat tgccccagtt
     8581 cacgctgttc ggcgcgcggc agataaggca gttcaccaat gagcggtgcc ggaagttttt
     8641 taccgagcac atcaatgatt tccgcataat gcgccagtcc tgggttgatt cggttagcca
     8701 cccagccaat gagcggcagc ccgtcgttgg caatcgcctg agctgttagc agtgcatggt
     8761 taatgcaacc ttcctgaata ccgacaacca tcaacaccgg cagttgttcc tgcaccaccc
     8821 attcagagag tggacgcaaa tcattcatca gactgcgcca gccgccagtc ccttctacca
     8881 cgacatgatc gactttttcg gtcaggtttg ccaggccgtt tgaaatgagg gtgtaattga
     8941 ttgggcaact gtgcgccacg ctactttctt cttcgcttaa cgcgatagga ttaactgctt
     9001 cataaggcag ttcgatggtt gaaacactct gcaacaccag ggcatcttta ttacgcagcc
     9061 cttcgggtgt ctctttgctc cccttcgcta cgggtttata tcccgcaacc gtttttccct
     9121 gggaggctaa cgcttgtagc aatgcgcggg aaaccaccgt tttccctaca gaagtgtctg
     9181 taccggtaat aaagaaacgc ttcagcatca ctaactccac cgttatgctt cacaaatata
     9241 aaccaggaaa ataattaacc ttgaaagtct aagttatgct ttcctggccc aaattgagat
     9301 agcgcaaatt ttggtagaac agttaaaaaa tgttaaccct gcaacagacg aatcaacaaa
     9361 gaaccgttat acatcgcgtc ttttaccagc gcagcgcctg ccatcgtgcc ctggttagaa
     9421 aactgagtac tctcaacgct aatgtgctga ctatacgcag gaagggcctg ctgacggatg
     9481 ctgtctgaga tgaccgggaa gaggatatct gccgctttac ttaacggtga gccaatcagt
     9541 attttttgtg ggttaaataa attcaccatg atggcaagaa tgcgccccac atgcgcgccc
     9601 accccggtaa tgatgtcttt tgccagcaga tcgccgcgca atgccgcctg acacaatgag
     9661 tccacggtta acggttgccc atgtaacatc gagctcatgg attgattgag acgcagctgt
     9721 gccagctcaa gtatactgtc cacactggcg atggtttcga ggcagccgtg attcccgcaa
     9781 taacaacgtt tcccatacgg gtcgacctgt gtgtggccta tttccacgag gctactgctg
     9841 cctgcgtgta gcagatgacc atcggtaatg acgcccgccc ccacgttgtg atcgataacc
     9901 acctgaatca catcgcgcgc cccgcgtgag gcaccaaaca aggcctctgc catcgtccat
     9961 gcgctgatat catgctgaat ataaaccgga acgccggtat gctgctccag agcctcgccg
    10021 agcggcatct cttttacatc ctcgtagaac ggcatgcgat gtacaatgcc attttccgta
    10081 tcaataattc ccggcagggt tatggcaatc gaagttagac gctcaagttt tttctggtgg
    10141 cggataaaaa actgatcgat atgggaaatg atacgatcca gcaatggcga gtcatctttt
    10201 aacgccagtt cctgcgactc ttccaccacc agtttgctgc tcagatcgcg cagagcaagg
    10261 aaaatctccc cgcgactaat gcgcagagaa agatagtgcc aggcttcagt ttcaaccacc
    10321 agccccaccg ccggacggcc acggttcccc gcttctttga tttccagctc ttgcaccagg
    10381 tgtgcttcga gcatctcacg gacaatttta gtgatactgg caggagccag ttgcgccaga
    10441 cgggaaagat cgatacgcga gactggacca agctgatcaa tcaggcgata aaccgcgccc
    10501 gcgttggtct gctttatttg atcaatgtgc ccaggctggt tttcagcaac caccgcatac
    10561 tccctatatt ttcgcgctcc gaaataatct gtaggctatg gtgaagcact tcaatacgtg
    10621 tcgtcaaatt tttacttagg catgtgatta acagcacatt tttcgggctt tttcgctgaa
    10681 atttccctca cctgagagca ttcagcagat gaatacgaaa gttacgcgca gctggacttt
    10741 gttcatgatg tttcggccag accaaccaca tttcagaaac cgcatcctct tctgcaatcg
    10801 gcacccagcg catttcgttg agctgaacac gtttaaatga agcaggcaaa attgaaacac
    10861 ccagaccggc ggaaaccaga ccgatgatgg tcattgcctc gcccacctcc tgagtgatga
    10921 cgggcgtcaa atggtaacgt cgcatcagcc cgagaatatc gtcatacagc cctgtcccga
    10981 cgtgcggatc aaaaaagacg aagggttctt tcgccagttc agccagcgtt acattcgggt
    11041 tatttgccag gggatgatcg tgcgggatca tcgccataag cggttcatgg acgatgactg
    11101 cgtgttcaag cgtctccggt aacgctgtgt tacgcagcaa tcccatatcc agcgttcctt
    11161 caatgagcgg agcgatttgc tcacgagtgt tcatttcgcg ggtttgtaaa tggacatcgg
    11221 gataatcacg gcgaaacagc gataacgtat cggacaccgc ccgaataaaa ggagcggacg
    11281 aagtaaaacc aatgcgcaac tcccccgctt ccccctgatg cagtctttca gcgcgagcgg
    11341 cagcgtcatc taccatagac aggatttgcc gactatctgc aagaaactgt tttcctgctg
    11401 ccgtcagcaa tacactgcga ttagttcgtg ccagcagtcg ggcaccaatt tgttgctcca
    11461 gtgcctgaat ctgctgactt agcggtggtt gcgaaatatt caggcgggca gcggcgcgcc
    11521 cgaaatgcag ctcttccgca acagca
//
LOCUS       DAFNBJ010000146        11517 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000146 DAFNBJ010000000
VERSION     DAFNBJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.146"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(26..577)
                     /gene="fliZ"
                     /locus_tag="L2X49_003834"
     CDS             complement(26..577)
                     /gene="fliZ"
                     /locus_tag="L2X49_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBN4798847.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(623..1342)
                     /gene="fliA"
                     /locus_tag="L2X49_003835"
     CDS             complement(623..1342)
                     /gene="fliA"
                     /locus_tag="L2X49_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBN4798848.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGKLEQELGRNATETEVAERLGIDIANYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1663..3675)
                     /locus_tag="L2X49_003836"
     CDS             complement(1663..3675)
                     /locus_tag="L2X49_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBN4798849.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSDTLNLAGFNVNGKGSVANTAATSDDLKLAGFTKGTTDTNGVTAYTNTISNDKA
                     KASDLLANITDGSVITGGGANAFGVAAKNGYTYDAASKSYSFAADGADSAKTLSIINP
                     NTGDSSQATVTIGGKEQKVNISQDGKITAADDNATLYLDKQGNLTKTNAGNDTAATWD
                     GLISNSDSTGAVPVGVATTITITSGTASGMSVQSAGAGIQTSTNSQILAGGAFAAKVS
                     IEGGAATDILVASNGNITAADGSALYLDATTGGFTTTAGGNTAASLDNLIANSKDATL
                     TVTSGTGQNTVYSTTGSGAQFTSLAKVDTVNVTNAHVSAEGMANLTKSNFTIDMGGTG
                     TVTYTVSNGDVKAAANADVYVEDGALSANATKDVTYFEQKNGAITNSTGGTIYETADG
                     KLTTEATTASSSTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEA
                     QSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            3941..5347
                     /gene="fliD"
                     /locus_tag="L2X49_003837"
     CDS             3941..5347
                     /gene="fliD"
                     /locus_tag="L2X49_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBN4798850.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRADTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDANGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            5372..5782
                     /gene="fliS"
                     /locus_tag="L2X49_003838"
     CDS             5372..5782
                     /gene="fliS"
                     /locus_tag="L2X49_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBN4798851.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            5782..6147
                     /gene="fliT"
                     /locus_tag="L2X49_003839"
     CDS             5782..6147
                     /gene="fliT"
                     /locus_tag="L2X49_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBN4798852.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWEELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            6225..7712
                     /gene="amyA"
                     /locus_tag="L2X49_003840"
     CDS             6225..7712
                     /gene="amyA"
                     /locus_tag="L2X49_003840"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBN4798853.1"
                     /translation="MRNPTLLQCFHWYYPAGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(7746..8159)
                     /locus_tag="L2X49_003841"
     CDS             complement(7746..8159)
                     /locus_tag="L2X49_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBN4798854.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            8346..9551
                     /gene="yedE"
                     /locus_tag="L2X49_003842"
     CDS             8346..9551
                     /gene="yedE"
                     /locus_tag="L2X49_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBN4798855.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMVVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            9548..9781
                     /gene="yedF"
                     /locus_tag="L2X49_003843"
     CDS             9548..9781
                     /gene="yedF"
                     /locus_tag="L2X49_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBN4798856.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            9890..10561
                     /locus_tag="L2X49_003844"
     CDS             9890..10561
                     /locus_tag="L2X49_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBN4798857.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQDIGGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(10546..>11517)
                     /locus_tag="L2X49_003845"
     CDS             complement(10546..>11517)
                     /locus_tag="L2X49_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006318378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBN4798858.1"
                     /translation="ATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQ
                     NDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTE
                     SEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKF
                     SNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTV
                     SQPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRCACCGHT
                     AKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGCQQTG"
BASE COUNT         2769 a         2909 c         2901 g         2938 t
ORIGIN      
        1 caactccgct acatcttatt cttatttaat atatatcaga agcaggcagg ctggaggatt
       61 tctgcacaag tccggtacac gtttgacgct gatagtgttg atacttccgt aatgcgatgc
      121 ggtaattgtt ggtgctggtg gtgggcagcc acggggcgag gatttcatca aggaaaccgt
      181 cctgcagctg atcgagggaa atattttgct cgtgtagatg attccccaaa cggcgcaaac
      241 gaacgacata ttcacgcacc gtacctgggc tcatttcagt ttgctcaaaa agaaattgct
      301 taaagccgat aatatcgaaa aaatcactct gcgttttgca atgtaaatca ccgcaaaatc
      361 ggcacaatgc cgcccatgat ttttgttccg tttgccagcc attttcatca agcagcgcgt
      421 ccaggcggga aatctcgatt ttattcacta ttttgccgtc gcgaacgagg gtaatgcgat
      481 cgagtaattt acggcaatgc gcgcaatgag tctggctgtg tttaaagtct ttaagatagc
      541 ggcttaatgg ccgtcttttc aggtgctgca ccatcattaa gaactcctgg tagtcaaaat
      601 taaagtgcgg catttactga cgttataact tacccagttt agtgcgtaac cgtttaatgg
      661 cctggctgtg taactgactg acccgcgatt ccccgacctc cagcaccgcg ccaatctctt
      721 tgagattcag ctcttcctgg taatagaggg ttaataccag tttttcgcgc tccggcaacg
      781 tttcgatagc ttccatcacc cgctggcgca gattactgtc cagcagttgt tgtagcgggt
      841 tttctcgctg atgatcatca gtaaccagtt cgatgctatc gccgtgctct tcgcgccact
      901 catcgtagga gaagagctgg ctgttattgg tgtcgagcag catttgacga taattggcaa
      961 tatcgatccc taaacgttcc gctacctcag tttccgtggc gttgcggcca agttcctgct
     1021 ccagtttccc tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg cgcggcaccc
     1081 agtcacggct gcgaagttca tccagcatag cgccacggat acgctgcact gcgtaagttg
     1141 taaatgccgt tccttgtagg gcgtcatagc gttcgacggc attaagtaac ccaatgccgc
     1201 ccgcctgtag cagatcgtca agttccacgc tcgcgggcag tcgaacctgc aggcgcaatg
     1261 cttcgtgacg caccagcggg acataacgct gccacagcga gtgtttatcc attacacctt
     1321 cagcggtata gagtgaattc acgataaaca gccctgcgtt atatgagtta tcggcatgat
     1381 tatccgtttc tgcagggttt ttaatcggac gattagtggg tgaaatgagg ggttatttgg
     1441 gggttaccgg taaattgcgg acagaaaaaa accccgccgg tggcggggaa gcacgttgct
     1501 gacaaattac cattcatgtt gccggatgcg gcgtaaacgc cttatccggc ctacaaaaat
     1561 gtgcaaattc aataaattgc agttcacctt gtaggcctga taagcgcagc gcatcaggca
     1621 atttggcgtt gccgtcagtc tcagttaatc aggttacgac gattaaccct gcagcagaga
     1681 cagaacctgc tgcggtacct ggttagcttt tgccagcacg gagttaccgg cctgctggat
     1741 gatctgcgct ttcgacatgt tggacacttc ggtcgcatag tcggcgtcct gaatacggga
     1801 ctgcgcttcg gacaggttgg tagtggtgtt attcaggttg gtgaccgcgg aatccagacg
     1861 gttttgcacc gcaccgaggg aggagcggaa tttgtcgatg gagctgatgg cttcgtccag
     1921 agctttcagg ggatcggcgg tggaactgga tgcagtagta gcttctgttg ttaacttacc
     1981 atcagctgtt tcatagatgg taccaccggt gctgttggta atagccccat ttttttgttc
     2041 aaagtaggtt acatcttttg tagcattggc tgaaagtgca ccatcttcga cataaacatc
     2101 agcatttgca gcagctttca catccccatt ggaaactgtg taagttactg tacctgtacc
     2161 gcccatatca atggtaaaat tgctttttgt cagatttgcc ataccttcgg cactgacatg
     2221 tgcgttggtg acattgactg tgtctacttt tgctaaactg gtgaactgag cgccacttcc
     2281 tgttgtgcta taaacagtgt tctggccggt acctgaagtt acggttaagg tagcatcctt
     2341 actgttagca attaaattat ctaacgaagc agctgtattt cctccagccg ttgtagtgaa
     2401 tccaccagta gtcgcatcaa gataaagtgc actaccatca gccgctgtta tgtttccatt
     2461 acttgctacc aaaatgtctg tagcagcgcc tccctcaata cttaccttag ccgcaaatgc
     2521 accacctgca agaatctgag aatttgttga ggtctgaatt cctgctcctg cggactgaac
     2581 agacattccg gaagctgtac cagaagtaat tgtaattgta gttgcaaccc caactggaac
     2641 cgcaccggta gaatcgctgt tggaaattaa accatcccaa gtcgctgcgg tatcgttacc
     2701 tgcattcgtt tttgtcaagt ttccctgttt atctaaatac agcgtcgcat tatcatctgc
     2761 cgcagtaatt tttccatcct gggaaatatt aactttctgc tctttaccac caatagtcac
     2821 tgtcgcctgc gacgaatcac cggtgtttgg attaatgatg cttaacgtct tcgctgaatc
     2881 ggcaccatct gcagcaaaac tataagattt acttgctgca tcataggtgt aaccattctt
     2941 tgcagccacg ccaaaagcgt ttgcccctcc cccagtgatc actgatccat cggtgatatt
     3001 agctaacaga tcggaagctt tggctttgtc attactaatt gtgtttgtat acgcggtcac
     3061 gccattggta tctgtggtgc ccttagtgaa accagccagt tttaaatcgt cgcttgtcgc
     3121 agctgtattc gctacagaac ctttaccgtt aacgttaaaa ccagccagat tcagcgtatc
     3181 agagtcaatt ttcttcagat caatagtaat cgtttcgcca tcgttcgcgc cgacctgaat
     3241 tttcatcgag ccatctttgg acagcacgtt cacgccgttg aactgggtct ggccggatac
     3301 gcggtcaatt tcgtccagac gggatttgat ttcgtcctga atggagtcca gatccgaatc
     3361 ggagttagtc ccggtagaag cctgaaccgt cagttcacga atacgctgta agttgttgtt
     3421 aatttcggac agcgcgcctt cagtggtctg tgcaacggaa ataccgtcgt tggcgttacg
     3481 tgcagcctga gtcaggcctt taatgttaga agtaaaacgg ttagcaatcg cctgacccgc
     3541 ggcgtcatcc ttcgcgctgt taatacgcaa gccagaagac agacgctcga tagaactcga
     3601 cagcgcagac tggttcttgt tgatattatt ttgagtgatc agcgagaggc tgttggtatt
     3661 aatgacttgt gccatgattc gttatcctat attgcaagtc gttgattacg tattgggttt
     3721 ccacccgtcg gctcaatcgc cgtcaaccct gttatcgtct gtcgtaaaac aacctttaga
     3781 atttttttca caaacagcca ttttttgtta gtcaccgaaa tactcttttc tctgcccctt
     3841 attcccgcta ttaaaaaaaa caattaaacg taaactttgc gcaattaagg ccgataaccc
     3901 cggtattcgt tttacgtgtc gaaagataaa aggaaatcgc atggcaagta tttcatcgct
     3961 gggagtcggg tcaggtctgg atttaagttc aatccttgat agcctcaccg ccgcgcaaaa
     4021 agcgacgcta acccccattt caaatcagca atcgtcgttt accgctaaac ttagcgccta
     4081 cggtacgctg aaaagcgcgc tgacgacttt ccagaccgcc aatactgcat tgtctaaagc
     4141 cgatcttttt tccgccacca gcactaccag cagtaccacc gcattcagtg ccaccactgc
     4201 gggtaacgcc atcgccggga aatacaccat cagtgtcacc catctggcgc aggcgcaaac
     4261 cctgaccacg cgcaccacca gagccgatac gaaaacggcg atcgccacca gcgacagtaa
     4321 actcaccatt caacaaggcg gcgacaaaga tccgattacc attgatatca gcgcggctaa
     4381 ctcatcgtta agcgggatcc gtgatgccat caacaacgca aaagcaggcg taagcgcaag
     4441 catcattaac gtgggtaacg gtgaatatcg tctgtcagtc acatcaaatg acaccggcct
     4501 tgataatgcg atgacactct cggtcagcgg tgatgatgcg ctacaaagtt ttatgggcta
     4561 tgacgccagt gccagcagca acggtatgga ggtctcggtt gccgcccaga atgcgcagct
     4621 gacggtcaac aacgtcgcca tcgagaacag cagcaacacc atcagcgacg cgctggaaaa
     4681 catcaccctg aacctgaacg atgtcaccac gggcaaccag acgctaacca tcactcagga
     4741 cacctccaaa gcgcaaacgg cgattaaaga ctgggtgaat gcctataact cgctaataga
     4801 taccttcagc agcctgacca aatacaccgc cgtagatgcg ggagctgata gccagagttc
     4861 tagcaatggc gcactgctcg gcgactccac gctgcggacg attcagacgc agttgaaatc
     4921 gatgctgagt aataccgtca gttcttccaa ctataaaacg ctggcacaga ttggtatcac
     4981 gaccgatccc agcgatggaa aactggaact ggatgccgac aaactcaccg ctgcactgaa
     5041 aaaagatgcc aacggcgtag gtgcattgat tgttggcgat ggtaaaaaaa ccggcatcac
     5101 aaccaccatc ggcagcaacc tgaccagttg gctttcgaca acgggcatta ttaaagccgc
     5161 taccgatggc gttagtaaaa ccctgaataa gttaactaaa gactacaacg ccgccagcga
     5221 tcgcattgat gcgcaggtcg cgcgctacaa agaacaattt acccaactgg acgttttaat
     5281 gacctcgtta aacagcacca gcagctactt aacgcagcag ttcgaaaaca acagtaattc
     5341 caagtaatca ttattcatcg ggagacaggt catgtacgcg gcaaaaggca cccaggccta
     5401 tgcacaaatt ggcgtcgaaa gcgccgtaat gagcgccagc cagcagcagc tggtcaccat
     5461 gctatttgat ggagtgctga gcgcactggt tagagcgcgc ctgtttatgc aggacaacaa
     5521 tcagcaaggc aaaggcgtct ctttgtcaaa agcgatcaac atcattgaga acggactgcg
     5581 ggtgagtctt gatgaagaga gcaaagacga attaacccaa aacttgattg ctctttatag
     5641 ctatatggtc aggcgcttgc tgcaagccaa tttacgcaac gatgtctccg cagttgaaga
     5701 agtggaagca ttaatgcgca atattgccga tgcctggaaa gagtcgttac tctccccttc
     5761 tttgattcag gacccagtct gatgaaccac gcaccgcatt tgtatttcgc ctggcaacaa
     5821 ctcgtcgaaa aaagccagct catgttacgc ctggcaacgg aagaacaatg ggaagaactc
     5881 atcgccagcg aaatggcgta tgtgaatgcg gtgcaggaga ttgcacatct gactgaagag
     5941 attgacccgt ccaccacgat gcaggagcag ctccgcccga tgctgcgcct gattctcgac
     6001 aacgaaagca aggtaaagca gttattacag attcggatgg atgaactggc gaaactggtc
     6061 ggtcagtcat cggtgcaaaa atcggtgtta agtgcctatg gcgatcaggg cggctttgtg
     6121 ctggctccgc aggataacct cttttgaatc tgaacgagtc gatggctcgc gaataatccg
     6181 attacggcta cgcttctaat gttccccttg aatggagtcg aagaatgcgt aatcccacgc
     6241 tgttacaatg ttttcactgg tattacccgg caggcggtaa gctctggcct gaactggccg
     6301 agcgcgccga cggttttaat gatattggta tcaatatggt ctggttgccg cccgcctata
     6361 aaggcgcatc gggcgggtat tcggtcggct acgactccta tgatttattt gatttaggcg
     6421 agtttgatca gaaaggcagc atccctacta aatatggcga taaagcacaa ctgctggccg
     6481 ccattgacgc cctgaaacgt aatgacattg cggtgctgtt ggacgtggta gtcaaccaca
     6541 aaatgggcgc ggatgaaaaa gaagctattc gcgtgcagcg tgtaaatgct gacgaccgta
     6601 cgcaaattga tgaagaaatc attgagtgcg aaggctggac gcgttacacc ttccccgccc
     6661 gtgccgggca atactcgcag tttatctggg atttcaaatg ttttagcggt atcgaccata
     6721 tcgaaaaccc tgatgaagat ggcatattta aaattgttaa cgactacacc ggcgaaggct
     6781 ggaacgatca ggttgatgat gaattaggta atttcgatta tctgatgggc gagaatatcg
     6841 attttcgcaa tcatgccgtg acggaagaga ttaaatactg ggcgcgctgg gtgatggaac
     6901 aaacgcaatg cgacggtttt cgtcttgatg cggtcaaaca tattccggcg tggttttata
     6961 aagagtggat cgaacacgta caggaagttg cgccaaagcc gctgtttatt gtggcggagt
     7021 actggtcgca tgaagtcgat aaactgcaaa cgtatattga tcaggtggaa ggcaaaacca
     7081 tgctgttcga tgcgccgctg cagatgaaat tccatgaagc atcgcgcatg gggcgcgact
     7141 acgacatgac gcagattttc acgggtacat tagtggaagc cgatcctttc cacgccgtga
     7201 cgctcgttgc caaccacgac acccaaccgt tgcaagctct cgaagcgccg gtcgaaccgt
     7261 ggtttaaacc gctggcgtat gccttaattt tgttgcggga aaatggcgtt ccttcggtgt
     7321 tctatccgga cctctacggt gcgcattacg aagatgtcgg tggtgacggg caaacctatc
     7381 cgatagatat gccaataatc gaacagcttg atgagttaat tctcgcccgt cagcgtttcg
     7441 cccacggtgt acagacgtta tttttcgacc atccgaactg cattgccttt agccgcagtg
     7501 gcaccgacga atatcccggc tgcgtggtgg tcatgtcgaa tggggatgat ggcgaaaaaa
     7561 ccattcatct gggagagaat tacggcaata aaacctggcg tgattttctg gggaaccggc
     7621 aagagagcgt agtgaccgac gaaaacggcg aagcaacctt cttttgcaac ggcggcagcg
     7681 tcagcgtgtg ggttatcgaa gaggtgattt aaattcatcc ccggcggcaa gccggggaga
     7741 tttcattacg gcagtggcgt cggtaacggc gctttatcca gcgcggcggc acactctgct
     7801 gtcgggcgat ccacacgctc catcgtcata ccatcgtact caatggtgtt accatcacgc
     7861 tcaacctcgt acagctcacg ttttacggta acgttagtga gatcatcaga catcaatgtc
     7921 aacttacccg gtacggcaat cacccgctgc cactgacggc aatcgagggt atcgccctct
     7981 ttagtgacaa tcaggctgcc tattgcttcc ggactgacca gcgcacgttg cggccctttg
     8041 gtctgccagt agcccgccag ccagtccggc gcgggggttt tcacgacatt gttatagttt
     8101 tcgacttcgg cacagcccgc cagcaacatg agcgcgcctg caattgcgag ttttttcatc
     8161 attcgtcctg catgagaaga aaagaggatt gtggcattaa agccctgatg tcgccagcct
     8221 ttccaggttc agagagaaat ttgatctaac tattattttt gtgaattatt accccgaaaa
     8281 taaatttgca tggtgtatga ttcgcgactc ttgtttcaaa ccttctgagt tcagaggcta
     8341 cattcatgtc atggcagcaa ttcaaacacg cctggttgat taaattctgg gcgcccatcc
     8401 ccgcggtcat cgcggcgggt attctctcca cttactattt tggcattact ggcacctttt
     8461 gggctgtcac gggtgaattt acccgttggg gcggccagct cctgcaactg ttcggcgtcc
     8521 atgctgaaga gtggggttac tttaaaatta tccatctgga aggatcgcca ttaacccgca
     8581 tcgacgggat gatgatcctc ggtatgtttg gcggctgctt tgccgcagcg ctgtgggcca
     8641 acaatgtcaa actgcgaatg ccgcgcagcc gtatccgcat tatgcaggcc atcattggcg
     8701 gcattatcgc cggttttggc gcgcgtctgg caatgggctg taacctggcg gcgttcttta
     8761 ccggtattcc tcagttctcg ctgcatgcct ggttctttgc catcgccact gccattggtt
     8821 catggtttgg cgcgcgcttt accctgctgc ccatcttccg tattcccgtg aaaatgcaga
     8881 aagtttctgc cgcctcaccg ctgacgcaaa aaccggatca ggcgcggcgt cgttttcgtc
     8941 tcgggatggt ggtctttttc ggcatgctgg gctgggcgct gctcacagcg atgaaccaac
     9001 ccaaactggg gctggcaatg ctgtttggcg tcggctttgg tttactgatt gaacgtgcgc
     9061 aaatctgctt tacttcggcg ttccgcgata tgtggatcac cggacgtacc catatggcga
     9121 aagcaatcat tattggtatg gcggtgagtg ccatcgggat cttcagttac gtacagttag
     9181 gcgttgaacc caaaatcatg tgggcgggac caaacgcggt aattggtggt ttactgtttg
     9241 gttttggcat cgtgctggct ggcggctgcg aaaccggctg gatgtaccgc gcggtagaag
     9301 gccaggtgca ctactggtgg gtcggtctgg gcaatgtgat cggctcaacg attctggcgt
     9361 attactggga tgatttcgct ccggcgctgg ccaccgactg ggacaaaatc aacctgctga
     9421 aaacctttgg tccgatgggt ggcctgctgg tgacatattt gctgttgttt gctgcgctga
     9481 tgttgattat cggctgggaa aaacgcttct tccgccgtgc ggcaccgcag actgctaagg
     9541 agatcgcatg aaaaatatcg ttcctgatta ccgccttgat atggtgggtg aaccctgccc
     9601 ttatccagca gtcgcaaccc ttgaggcgat gccgcagtta aaaaaagggg aaatcctgga
     9661 agtggtgagc gactgtccgc agtcgatcaa taatattcca ctggatgcgc gtaatcacgg
     9721 ctatacggtg ctggatattc agcaagacgg gccgaccatt cgttatttaa ttcagaagta
     9781 atccctcatt cccgctgtga cccacagcgg gaacatcgtt acctttgtat cacatagatt
     9841 ttacccaccc cctctcctat tcttttcagt atgatctcag gaggtttcta tgtgtggacg
     9901 ctttgcccaa tcccaaacgc gtgaagatta ccttgcgctt ctcgcggaag atattgaacg
     9961 cgatattccc tacgatcccg aacccattgg cagatacaac gtcgcgccgg gaaccaaagt
    10021 tcttctgctc agtgaacgtg atgaacacct tcatctggat ccggttttct ggggatatgc
    10081 tcccggatgg tgggataaac cgccgctgat taacgcccgc gtagaaactg cggccaccag
    10141 tcgtatgttt aaaccgctct ggcaacatgg tcgggcaatc tgttttgccg atggctggtt
    10201 tgagtggaaa aaagaaggcg acaaaaaaca gccttatttt atctatcgcg ctgatgggca
    10261 acctattttt atggccgcga taggtagcac accatttgag cgtggtgacg aagccgaagg
    10321 atttttaatt gtcactgctg cagcagacca gggtctggta gacattcatg accgccgccc
    10381 actggtactg tcgccagaag ctgcgcggga atggatgcgg caagacattg gcggtaaaga
    10441 agcctcagaa atcgcggcca gtggctgtgt gccagcaaac cagttctcct ggcacccggt
    10501 atcgcgcgcg gtaggtaatg tcaaaaacca gggcgcggag ttaattcaac ctgtttgttg
    10561 acatcctcca caggcaagaa cggcgtgccc cgccgctaaa atgttacgag cgccgtttac
    10621 atcggcgttc gctgtatatc cacatacctg gcatctgaat ttactttgtg acaggcggtt
    10681 ttctttcgct gtatgaccac agcacgcgca acgctggctt gtgtacgctg gcggaacagc
    10741 aagcacctga ccgccacgcc agagctgctt gtacgcaagc tggcggcgca tttcatacca
    10801 gccctgatcc agtatcgaac ggtttaaacc tgattttgcc cggacattgc gccccggctg
    10861 gctgaccgta cccgctgctg actttgacat gttgctgacc ttcaaatcct caatgactat
    10921 cattgcgtgg tttttgctga cggtcgttgt gactttgtga aggtagtccc tgcggatatt
    10981 tgcgatacag gaatgcagtc gctgtatttt gcgtttctgc ttctgccagt tgttgctgaa
    11041 tttgaccttg cggcttaact gtcgctgaag tctcgccagc ttcttctggt ttttctgaaa
    11101 actgtttaca ggctcaaaga ctgtgccatc tgacagcgtg gcgagtttag ccacgccagc
    11161 atccagcccg accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgtctg
    11221 aatactgatg taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt
    11281 gacctgacgg ctgttccggt agcgcatcca gcccagtttc ggcagaaaaa tacggctgtt
    11341 ttcctgatcg agcttaacgc cctgcgggta gcggaataca tcattctgtc cccgcttttt
    11401 gaatcgggga aaagcagccc gattctggaa gaagtttttg taagcccgct caaggtcttt
    11461 cagtgactgt tgcaatggct gtgacggggc atctttaagc cattgcgttt cagtggc
//
LOCUS       DAFNBJ010000147        11451 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000147 DAFNBJ010000000
VERSION     DAFNBJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.147"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(474..1727)
                     /locus_tag="L2X49_003846"
     CDS             complement(474..1727)
                     /locus_tag="L2X49_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBN4798859.1"
                     /translation="MPDKREQMTYASQAVKRTPHEVTDHFIKMVHARIAEVSGWRYVF
                     DRIPAFKDACDKAPGQVPCPFSGVGKSKFRFRKKDLFTGCAIHNDFPVNAFCDGIDVL
                     AEYYKLSKTQTCKKILTDFFGMDLYAPLTDADLESERRYKSTVRATETLDSDEVEKRG
                     RKLEVIYHYTGEIKPESPVWVYLRNRGLNRVLSNLPKDLGLNKRLYYMDKSLEKPTIY
                     PGMIAIYRDTRGRPLTIHRTFVELNGDKAHVENPKLMMKPPADMTGGSIQLYDPHFNP
                     GTRTWTLGVAEGIENALSVTEATSTPCWAASSAWCLENVEVPDSLLPPPGVKVIQFYI
                     WADKDLVNTKGTSPGMESAKRLQERMKEFFAKRYPTSELIIKVFEPDFDIPVGKKGVD
                     WNDVLKLTGPDGFPVKWAPECLAQL"
     gene            complement(1821..2042)
                     /locus_tag="L2X49_003847"
     CDS             complement(1821..2042)
                     /locus_tag="L2X49_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798860.1"
                     /translation="MTQIKTYRVEYEKVGTMHRVRIFGRMGEIVKSELPEERILRDVS
                     IPEGNGEMATSMVDGFIQRLENIGFKTEA"
     gene            complement(2114..4126)
                     /locus_tag="L2X49_003848"
     CDS             complement(2114..4126)
                     /locus_tag="L2X49_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBN4798861.1"
                     /translation="MKFDLKSSPRHIQRLTNIASVISGINGIYVIIDNKVSTPYFNTQ
                     NNVCVLPNGDYSDERFVKLIEGFICHEAGHGRYTEHEVYREAFVGELINADGFISIDD
                     DLKADFQNLKQKQKAYARACRLKGLINLFDDVQMEEKTGIDYQEAKKRLAVTYALMVE
                     AGRMTVDISSTPQNPVQFIEMYLLNTLRVNVLQQEGHKETLDPFFDYAKKILAPVTSE
                     VDEIIHQALSCKSTQNCDSLARKTLALLERLRDEAKEKQQEEEQSKDPHDDTDESSGS
                     ENEPDTEPNGDSQGEGDGDKEGQGDDSGDGKAPAEGNGLPDQSGGDEQNAENSNGEPD
                     GESEGDESATPGSDDADSTPSLNDSSNGNSSDGESNFSPEQWDMLAKLLDDFLNSDEE
                     SEDFHEVLAKEISVIAASVSDEVKAEFGASEWDVPDLNIDLNVYNEALNISQTLGADL
                     SVLQQVKMRGQFKTRDRGLSFDINRLIQSPMGVRDVFRSQSESKNRGHVGLVIVRDIS
                     GSMSLEHRYIHAIKTDLALTLAIEAISKMHVANVIYPFVDKDFEVIKTFDENAEEKLS
                     KFSLGCKGNNTPTGSALNAALELLLESQFDRKIVFLITDGYPTESAYTINDVFSVAES
                     NGIEIAGVGIKTDVLMGFNEGTFVNVDDISLLPNEVSKLVHQILSN"
     gene            complement(4204..5331)
                     /locus_tag="L2X49_003849"
     CDS             complement(4204..5331)
                     /locus_tag="L2X49_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3150 domain-containing protein"
                     /protein_id="HBN4798862.1"
                     /translation="MTFNTSKLNKVSAKEISENFIKAYPDLLEGAVITKIEISGCQGS
                     RRTDKIELSYGGDDITDQKSVSKSEVRWIDIKALSFKSTITSRISTLCSRLCIRYSNM
                     WILPVERMEEFLEEAQEIEREFQAGIQNVVDNYDTHIQAEKDRSPLMSDLIDQLKLTK
                     DDFVKSFRFNLAHFIPFSPISVEGDETQDFYQEQLITDLADEAMKVYTKISKNDNLRS
                     STIDRLKQMQDKIISFMFIHKEAVVLAEAIKHIMNNLPKGAISSPRDVAVLKQWFYFM
                     SDASMLKRIISGEQKVTDWLDSITRSFNIDSQVTAANSLNTAEDDVFSSANVFREDPV
                     EDLENKAVTAPEPAVGPDEDVSIQASGSNRAFDCELELTGW"
     gene            complement(5402..5671)
                     /locus_tag="L2X49_003850"
     CDS             complement(5402..5671)
                     /locus_tag="L2X49_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798863.1"
                     /translation="MTDFAHLTFYGFLSANGTETFVIAQDGNQYIGLKQHNNDYSFIC
                     YEVCNKDMEAMIKGLESKGFMYQGSIQSADVENEFPGILLTTLEK"
     gene            complement(5710..6762)
                     /locus_tag="L2X49_003851"
     CDS             complement(5710..6762)
                     /locus_tag="L2X49_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN4798864.1"
                     /translation="MTNAATVLKSENSVQEEIIGNLLPANVISSKMFANKEQYYLVKA
                     KDLFGIPEMDEDMIVPVFKKFSPYRANLNKDYIPNSRILEQVVKLLVSNDIDLSVCLK
                     GESGSGKTEMAMYISHMLNWPLTIKQINSNIRADELEGERSLINGNTSFVPSELVTAF
                     QEGHLILLDEVDKIDPDTAAKLHMPIERKPWSLAANGGEVVKANKFTRFMGTSNTNMS
                     GGSRKFVSSQRQDAAFIKRFLIVEMERPSEVALTNVLLKRYNNLPMLVIEKFVSVAVA
                     VNNTGTDDSVMDIRQLVAWVGTSMTLKTMSLLDTFKIAFASALPAHATGMVLEAIDLI
                     LGDDKNRTMEYYTAKK"
     gene            complement(6822..7808)
                     /locus_tag="L2X49_003852"
     CDS             complement(6822..7808)
                     /locus_tag="L2X49_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798865.1"
                     /translation="MKQKIRPTAFCSKQMDFFSIFDFEEEEKEIITIVESPLDYINDA
                     IAAQQSIQSEDKHSGPELEPSVSEDVALPGNCPKLLKNQILHDISFAAETDYLFTLEE
                     YSAELGGLVIDWAPEDVYQLYVVAMEESLENVRHLVITKSLYSTDEFGNITVNPLLEA
                     ETRWYMSKSFELTCATHGIDAIEFRSELKKSLYEYTHSYGGENAELARYHQDKEVILH
                     DCKEEMGWDIFFEQDYLLQENKLAMKWTDRDIMDVYSKVFKSTINLFEKLVVNNKLTL
                     RDTFGWVIVNPTFERQFEWIDSEVFEIVGTHLGYNVAAIRTQMATACRMTFH"
     gene            complement(7869..8246)
                     /locus_tag="L2X49_003853"
     CDS             complement(7869..8246)
                     /locus_tag="L2X49_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798866.1"
                     /translation="MSYLEYVNEVKVSGIVISAMEKEMTSGNVGLLIKLKNRMKTEIN
                     GEITEREFSIQIKVSPEMYSGCFTGINQGDELMVSGYLVVDTITLEGREHPLDYMRVV
                     ATSKLAHIPKPPKGFGQSSFRQI"
     gene            complement(8305..9180)
                     /locus_tag="L2X49_003854"
     CDS             complement(8305..9180)
                     /locus_tag="L2X49_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000045554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798867.1"
                     /translation="MSKSKARSKALLIAFADLIPDMDKVVNKKLLDSLNIYSGHDNDL
                     IVIMNEDGPTIIELNSLKSVSMLAQKLSAFSTYYHVEMQQILVNPIDFEKAYTLLKEA
                     PAIPMFKTLADLDKFLNEEFEKYGLNTFLDVDNLDYSLAKSRELKNDQLVAWVSEIIE
                     KREKLALRNRFNEVTKAHYETVDAMYAAVRPLMKELGFPDELMLHTFSELSVFDSKGW
                     DYAIKSKIEFLTKREEQCLDYQMKADKRQATVDELLAQINNAKTVKAPRSFGQLFGFS
                     VIAMMTFMFIVNKFI"
     gene            complement(9578..11362)
                     /gene="trhI"
                     /locus_tag="L2X49_003855"
     CDS             complement(9578..11362)
                     /gene="trhI"
                     /locus_tag="L2X49_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer helicase TrhI"
                     /protein_id="HBN4798868.1"
                     /translation="MGKPTDEQRVIIENANANNMVIAAPGSGKSFTMIEAVISILRQF
                     PYAKVGMVTFTRAATNSLAEKLKRRLSKKDQDRVLVNTFHGFIRMQLDMVNWKGKMLI
                     SSAQRSVIHRALKESGAPFRYPDAEFAIDAIGREMDTDIISVRHTRQQIHLFNTYQAI
                     CQKDHVADLNALSRFVVGQMYSGKMQPLNLTHLVVDEVQDTDSIQYAWISLHTRAGVN
                     TSIVGDDDQAIYSFRASGGVKIFQQFEKQFRPNIFYLNTCFRCEPEILKVAGALIEKN
                     VYRYAKDLRSAKGGGGKVHFRSYVDMDEQIQGILNLINQDPIGWAILSRGNAHLDQLE
                     SLIEQPVLRYGGKSFWDEKETSDVLHLMAFFRHSNDVRLMKRVLALFGENEEVLDQTA
                     LSMKGRKVTFGELNIPNESSLETRTLHSNFTRFTQETREKVEIEKRFANLIKWMELSS
                     IKMRTQKGSPSLSRIALDTCKQWAEKTGWQNMINRAAAMCLGPKKKDEEYTPDKVVLS
                     TLHGSKGLEWKNVIIMSCNADQIPSKRSVGQEAIEEERRLLFVGFTRAEQQLHVMWYG
                     DPSFFLSECAEDKLKEAAKSRTESTLTE"
BASE COUNT         3217 a         2650 c         2089 g         3495 t
ORIGIN      
        1 tatcacaacg caacaaacag gttcctgaag gacgaataac gtacaacagg tttttgtctt
       61 tatctattat gctgtgcttt aaaatcgtaa agtcaatttc actatcatga aggtaataac
      121 acgatgatgg ttgctcaact atacgagaag aaatcacagc agcgaactta ttaaataaat
      181 cttgattttc aatattcata acaatgctct ccgatattaa acgatgagca tcaccctaac
      241 ggtaatactc ccgtcagggg ttttaagttt tatggatata ccatgtcata caaccgaccg
      301 tagccagatg tatcgcacat ttattaaact gagcaagcat ctcagccgta aaaaaaacct
      361 gaataacagg tgattttcac gaaggaaatc ggattatctg tttaggattt aacgtaaata
      421 aacactaaag agataacccc ccccggcctg aaaaggcggg ggagttatcg gatttaaagc
      481 tgagcaagac attcaggagc ccattttacg gggaatccat ccggtcctgt taattttagt
      541 acatcgttcc agtcgactcc tttcttccct acaggaatat cgaaatcagg ttcaaagact
      601 ttaattatca gctctgaagt gggatatcgc tttgcaaaaa actctttcat acgttcctga
      661 agtcgtttag ctgactccat tcccggggaa gtgccttttg tattcacaag atccttatcc
      721 gcccagatgt aaaactgtat tactttcaca ccagggggag gcaggagact atccggcact
      781 tcaacgtttt caaggcacca ggcagagctc gcggcccagc acggtgttga agtcgcttca
      841 gtaactgaca gcgcgttttc tatgccttca gccacaccta acgtccaggt acgggtacct
      901 ggattgaagt gtggatcgta cagctgaatt gagccgccag tcatatcggc aggcggtttc
      961 atcatcagtt tcggattctc aacatgtgcc ttatcaccat ttaactcgac gaacgttctg
     1021 tggattgtca gaggacgccc acgggtatcg cgataaattg cgatcattcc cgggtaaatc
     1081 gtgggttttt ccagtgactt atccatgtag taaagtcgtt tgttcagacc taaatccttg
     1141 ggaaggttgc tcagaactcg attaagacct cgatttcgga gatacaccca cacagggctc
     1201 tcaggtttga tttcaccggt atagtgatat atcacttcaa gtttacgccc tcgtttttca
     1261 acttcatcgc tgtcaagcgt ttcggttgca cgaactgttg atttataacg acgttcactt
     1321 tcgagatccg catcagttaa tggagcgtac aaatccatcc caaagaaatc agtcaggatc
     1381 ttcttacaag tctgggtctt gctcagcttg tagtactctg ccaatacatc aataccgtca
     1441 caaaatgcat tcactggaaa atcattgtga atggcacaac cggtaaagag gtccttttta
     1501 cggaaccgga actttgattt cccgacccca ctaaatgggc aaggaacctg gccaggtgct
     1561 ttatcacaag cgtctttaaa tgccggtatt ctatcgaata catagcgcca gcctgatact
     1621 tccgctatac gagcgtgaac catttttata aagtggtcag tgacttcatg tggtgtacgc
     1681 ttaacagcct gggatgcata agtcatttgt tctctcttat ctggcattgt gggtttctca
     1741 atgccctctg cgatgcagaa aacagggaga aacccacaac gcagaatcta agaatgaggg
     1801 cccacaaggg ccctcaacaa ttacgcttca gtcttgaagc cgatattttc aagacgctgg
     1861 ataaaaccat caaccattga cgttgccatt tctccattac cctctggtat agatacatcc
     1921 cggagaattc tttcttctgg caattcactt ttgacaattt cacccatgcg accaaaaata
     1981 cggacacgat gcatcgtccc gactttttca tactcgacac ggtaagtctt tatctgagtc
     2041 atatattgct ccttaatcct tcagggagca aagcgaccca accgggcgtc gttttgcccc
     2101 gattgggtag agtttaatta gatagaattt gatgtactaa tttactgacc tcattcggca
     2161 ataaagaaat atcatcaaca tttacgaagg tgccctcatt aaaacccatc aacacatcag
     2221 ttttaatacc aacaccagcg atttcaatgc cgttactttc agcaactgaa aaaacatcat
     2281 taatggtgta agccgactca gtcggataac catcagtaat aaggaaaaca attttcctgt
     2341 caaactggct ttcaagtaac aactctaaag cagcatttaa tgcagagccc gttggggtat
     2401 tattgccctt gcagccaagt gagaattttg aaagtttctc ttccgcgttt tcatcaaacg
     2461 ttttgataac ttcaaaatct ttgtctacaa acggataaat cacattagca acatgcattt
     2521 ttgaaatagc ttcaattgcc agagttaatg caaggtcagt cttaattgca tggatataac
     2581 ggtgttccag actcattgaa cctgaaatat cacgaacaat gaccaagccg acgtggcccc
     2641 ggtttttaga ttcgctttgt gaccgaaata catccctaac acccatcgga gactgaatca
     2701 gtcgattaat atcgaatgac aagcctctat cacgggtttt aaattgacca cgcattttta
     2761 cctgctgtaa aacagataaa tctgccccaa gggtctggct gatatttaat gcttcattgt
     2821 agacattcag atcaatgttc aaatctggaa catcccactc acttgcacca aattcagctt
     2881 taacctcatc cgacacactg gcagcaatta cggagatctc ttttgcaaga acctcatgaa
     2941 aatcctcgct ttcctcatca ctgtttaaaa aatcatccag caatttggca agcatatccc
     3001 actgttcagg agaaaagtta ctttccccat cagaagaatt tccatttgaa ctgtcgttta
     3061 gggaaggtgt cgagtcagca tcatccgagc cgggggtcgc cgattcatca ccttcagact
     3121 caccatcagg ttcgccgtta gagttctcag cattttgttc atcccctcct gattggtccg
     3181 gtaacccatt accttccgca ggcgccttac catcaccaga atcatcgcct tgaccttctt
     3241 tatcaccatc cccttcaccc tggctatcac cattgggttc ggtatcaggt tcattctcag
     3301 agccagatga ttcatctgta tcgtcatgcg ggtctttcga ctgctcctct tcttgctgtt
     3361 tctcttttgc ttcatccctt aaacgctcta aaagagccag agttttacgc gccagagaat
     3421 cgcaattttg cgtacttttg caacttaaag cttgatgaat gatctcatcg acttctgaag
     3481 ttacaggtgc aaggattttc tttgcataat cgaaaaatgg gtcaagcgtt tctttatgcc
     3541 cttcttgttg gagaacgtta acccgcaatg tattaagcag gtacatttca ataaactgaa
     3601 ccgggttttg aggggttgaa gaaatatcaa ccgtcattcg cccagcttca accatcaagg
     3661 cataagtcac cgcgaggcgt ttttttgcct cctgataatc aatacccgtc ttctcttcca
     3721 tctggacatc atcaaaaaga ttaataagtc cttttagtcg acaagcacga gcatatgctt
     3781 tttgcttttg ttttaggttt tgaaaatcag ccttcaagtc atcatcaata ctgatgaagc
     3841 catctgcatt gatcaattct ccaacaaaag cttcacggta tacttcatgc tctgtataac
     3901 gaccgtgtcc tgcttcatga catatgaatc cttcaattag cttgacaaat ctctcatcgc
     3961 tataatcacc gttaggtaaa acacagacat tgttttgggt gttgaaataa ggtgtactca
     4021 ccttattgtc aataattaca taaatcccat ttataccgct tatcacgctg gctatattcg
     4081 tcaggcgttg aatatggcgt ggtgaagatt ttaaatcaaa tttcatttga gtacctttat
     4141 ttgataaagg gtactcaccc agatggtgaa gtacccatct ggggatattt ttacaagact
     4201 aaattaccaa ccggtcaatt cgagttcgca gtcaaatgct ctattactac cactggcctg
     4261 aatagaaaca tcttcatcag ggccaacagc aggctcaggt gcagtcactg ccttgttctc
     4321 aagatcttca accgggtctt ctctgaaaac atttgctgaa gaaaaaacat catcctcagc
     4381 agtattcaga gagttagccg ctgtaacctg tgaatcgata ttgaatgacc gggttatgga
     4441 atccagccag tcggttacct tttgttcacc ggagataata cgtttcagca ttgacgcatc
     4501 actcatgaaa tagaaccact gctttaatac cgcaacatca cggggagaag aaatggcacc
     4561 ttttggtaaa ttattcatga tatgcttaat agcttctgca agaacaacag cctctttatg
     4621 aatgaacatg aagctaatta ttttatcctg catctgcttt aatcggtcaa tcgtgctgga
     4681 acgcaaatta tcatttttgc tgatttttgt ataaaccttc atagcttcat cggccaagtc
     4741 agttatcagt tgttcttgat aaaagtcctg cgtttcatcc ccctccacac taatcggtga
     4801 aaacggaatg aaatgtgcca gattaaaccg gaatgattta acaaaatcat ctttagtcaa
     4861 tttcaactgg tcaattaaat ctgacatcaa cggactgcga tctttctcag cctgaatatg
     4921 agtgtcatag ttatcaacca cgttctggat accggcttga aattcacgtt cgatttcttg
     4981 tgcctcttcg agaaactcct ccatcctttc aactggcagt atccacatat tgctatagcg
     5041 gatgcacaac cgtgaacaaa gggttgaaat tcgactagtg atagtgcttt tgaaactcag
     5101 tgcctttatg tctatccagc gaacttcgct tttagacacg ctcttctggt ctgttatatc
     5161 atcaccgcca taggacagtt cgattttatc agttcgccga gagccctgac acccactaat
     5221 ttcaattttt gttataacag caccttccag caagtcagga tatgctttta taaagttttc
     5281 actgatttcc tttgcagaaa ctttattaag tttagaagta ttgaaagtca taggttttct
     5341 ccagatttag aaatgggaaa acctccccgt cgggaaagat ttcccgatag ggttaataat
     5401 attacttttc aagtgttgtt aaaagaattc caggaaattc attttcgaca tccgccgatt
     5461 gaatgctccc ttgatacatg aatccttttg attcaaggcc cttaatcatg gcttccatat
     5521 ctttgttaca gacttcataa caaataaatg aatagtcatt attatgctgc ttcagaccta
     5581 tatattgatt tccatcttgt gcaatcacga aagtttcagt tccgtttgca ctcagaaaac
     5641 cgtaaaacgt aagatgagca aagtctgtca cacggcattc agccttctga taactcgtcc
     5701 catctataac tacttttttg cagtgtaata ctccattgta cggttcttgt cgtcaccgag
     5761 aattaagtcg atggcttcca acaccatacc agtggcatgt gcaggcagag ccgacgcaaa
     5821 agcaatttta aatgtatcca gtaaggacat ggtctttaac gtcattgacg ttccaaccca
     5881 tgccacaagc tggcgtatat ccattacact gtcgtcagtt cctgtgttgt ttactgcaac
     5941 ggctaccgaa acgaattttt caattaccag cataggcagg ttattgtagc gtttaagcaa
     6001 aacgttggtt aatgcaacct ctgaaggcct ttccatctcc acaatcagga agcgcttgat
     6061 aaacgctgca tcctgccgct gcgatgaaac gaacttacga gatcccccac tcatgtttgt
     6121 gtttgaagta cccataaacc gcgtaaactt atttgcctta accacttcgc caccgttggc
     6181 agctagtgac cagggctttc gctctatagg catatgaagc tttgcggcag tatcagggtc
     6241 aatcttgtcc acttcatcca gaagaatgag gtggccttcc tgaaatgccg ttacaagctc
     6301 gctaggcaca aaactggtgt taccgttaat aagactacgc tcaccttcaa gctcatcagc
     6361 gcgaatattg ctgttgattt gctttatggt tagcggccag ttcagcatat ggctaatata
     6421 cattgccatt tcagtcttac ctgatccaga ttctcctttc aggcatacac tcaaatctat
     6481 atcgttactt acaagcaatt ttactacttg ctcaagtatt cgagaatttg gtatatagtc
     6541 cttattcaga ttcgcacgat atggagaaaa ctttttaaac actggaacaa tcatatcctc
     6601 atccatctca ggaatgccaa ataaatcttt tgctttgaca agataatact gttctttatt
     6661 cgcaaacatc ttacttgata taacatttgc gggtaacaga ttaccaatga tttcttcctg
     6721 tacggaattt tcggatttta atacagttgc ggcgttagtc atttttaaaa ctccggtaaa
     6781 atgcaaacac cgcccgctgg cagatgtttg ccagtggcat gttagtggaa agtcatccta
     6841 cacgccgtcg ccatctgtgt tctgatagcg gcaacgttgt aaccaagatg agttcctacg
     6901 atttcgaata cttctgagtc aatccattca aactggcgtt caaatgtagg gtttacgatt
     6961 acccaaccaa aagtatctct taaggttaac ttgttattaa cgacaagttt ttcaaacaaa
     7021 ttaattgttg atttaaatac tttagaatag acatccatta tatcccgatc agtccatttc
     7081 attgccagtt tgttttcttg caacaaatag tcctgttcga aaaagatatc ccatcccatc
     7141 tcttccttac aatcatgcag aataacctct ttatcttgat gataacgagc taattcagca
     7201 ttttcacctc cgtaactgtg cgtatattca tataagcttt ttttaagctc tgacctgaac
     7261 tcaatagcat caataccatg agtggcacat gtaagctcaa atgatttcga catataccac
     7321 cgtgtttcgg cttcaagaag cggattcacg gttatgttac cgaattcatc agtggaatag
     7381 agtgattttg taatgacgag atgccttacg ttttccaacg actcttccat tgcgacgacg
     7441 tataactgat atacatcttc aggggcccag tcaataacca gaccacctaa ctccgcactg
     7501 tactcttcta gagtgaagag ataatcagtt tccgcagcaa aactaatgtc atgcagaatt
     7561 tgatttttga gaagtttggg acaattacca ggcaaagcaa catcttccga cacactaggc
     7621 tccagttccg ggcctgaatg tttatcttct gattgaatac tttgttgtgc ggctattgca
     7681 tcatttatat agtcgagtgg agactcaact atagttatta tttctttttc ctcttcttca
     7741 aaatcgaata tagagaaaaa atccatttgc ttactgcaaa atgcagtcgg ccttattttt
     7801 tgtttcatgg gatctcctaa gggagaactc ccatgaaggg atttctcccc ttttgggtgg
     7861 tttaaatgtc agatctgtct aaagctactt tgcccaaaac ccttaggagg tttaggtatg
     7921 tgagctaact tacttgttgc aactactctc atgtagtcaa gaggatgctc tctcccttcc
     7981 aatgtgatgg tatccacaac aagataaccg gagaccatta actcatcacc ttgatttata
     8041 ccagtgaaac agcctgaata catctctggt gatactttga tttgaatcga aaattctctc
     8101 tcagtaattt caccattaat ttcagttttc attctgtttt tgagctttat caataaccca
     8161 acgtttccgc tagtcatttc tttctccatt gcactgataa ctatcccgga cacttttact
     8221 tcattaacat attcaagata tgacattact tctctcctca atttaagcaa agagaagccc
     8281 cgtcagggtt tccccttaac aggattagat aaatttgttt acaatgaaca taaatgtcat
     8341 catggcaata acagaaaagc caaacagctg accaaaagat cttggtgctt ttaccgtttt
     8401 agcgttattg atttgtgcca agagttcatc aactgtcgct tgccgtttat ctgctttcat
     8461 ttgataatct aaacattgct cctcgcgttt tgtgagaaac tcaattttcg acttgatagc
     8521 ataatcccag cctttggaat cgaaaacgct caattcacta aaggtgtgta acatgagttc
     8581 atcaggaaaa cctaactctt tcattaatgg gcgaacggct gcatacattg catctacagt
     8641 ttcataatgt gctttggtca cttcattaaa tcgattacgc aatgctaatt tctcgcgctt
     8701 ctcaataatt tcagaaaccc aggcaaccaa ctgatcattt tttaactctc tggatttagc
     8761 gagtgaataa tcaaggttat ccacatcaag aaatgtgttc aacccgtact tttcaaactc
     8821 ttcatttaag aacttatcca gatcggctaa agttttaaac attggaattg caggtgcttc
     8881 tttgagaagt gtgtaagctt tctcaaagtc aattggattg acaagaattt gctgcatttc
     8941 cacatgataa taagtagaaa aagcagaaag cttttgagcc agcattgaaa ctgatttcaa
     9001 agaattcagt tcaattatgg ttggaccatc ttcattcata ataacaataa ggtcattatc
     9061 atgccctgaa taaatattca gagaatccaa taacttcttg ttaaccacct tatccatatc
     9121 tgggatcaga tcagcgaagg cgatgagaag agctttactg cgtgctttac ttttagacat
     9181 ttggggtcac ctatatatag taagaaggaa ggaatcatta aatatcccaa cacaaaggca
     9241 aagcctgtca ttcatctccc gtaagctatg aaccgcctga tttatacttt ctggcattag
     9301 aagtacgcaa cgaaaatagc tcaattaaga gctaagttag ttacgatttg gtaaattcga
     9361 ttgtcctttt tcagacatgg gaaaggcacc agcgacaaag gtgacatttt gccggtggtg
     9421 ccttgcagct ttagaaagct ggccgctttt cacggctgaa tatgcgctta acgcgcaaag
     9481 taacttttat aaagttactt gaaatgtggg agcaataaat tcaagctcat atacgttata
     9541 ctacacgggt tcattcctgt aaataaagaa atcaaaatta ttctgtcaaa gtggattctg
     9601 ttctactttt cgcagcctct ttcaatttat cttcagcaca ttcgcttaaa aagaaagacg
     9661 gatcaccgta ccacattaca tgcagctgtt gctctgctcg tgtaaaccca acaaaaagca
     9721 gtcgtctttc ttcttcaatc gcctcctgcc ccactgaccg cttacttgga atctgatctg
     9781 cattacaact cataataatt acatttttcc attcaagacc cttcgaaccg tgcaacgtcg
     9841 ataaaactac tttatccggt gtatattcct catccttctt ctttggcccc agacacatcg
     9901 ccgcagctcg atttatcata ttttgccagc ctgtcttttc ggcccattgc ttacaggtat
     9961 ccagcgcaat tctgctcagt gatggtgatc ctttctgcgt cctcattttt atcgacgaca
    10021 actccatcca cttaatcaga tttgcgaacc ttttttctat ttcaaccttt tcccgagtct
    10081 cctgtgtaaa ccgggtgaag tttgaatgaa gcgttcttgt ttccagggaa ctttcattcg
    10141 ggatgttcag ttcaccaaaa gttacttttc ttcctttcat gctcaatgct gtctggtcca
    10201 agacctcttc attttcaccg aaaagcgcca gcaccctctt cattaaccgg acatcgttgg
    10261 aatgtcggaa gaatgccatc aaatgcaata catcgcttgt ctccttttca tcccaaaatg
    10321 acttcccacc ataacgaagt accggctgct ctatcaggct ttcaagctga tcgagatgtg
    10381 cattacctct gctgaggatt gcccacccaa tcgggtcttg gttaatcaga ttgagaatgc
    10441 cttgaatttg ctcatccata tcaacatatg agcggaaatg aacttttccc ccaccgccct
    10501 tagctgaacg gagatcttta gcgtatctgt aaacattttt ttcaatcaat gcccctgcta
    10561 ccttcaaaat ctctggttca catctgaaac aagtattcaa ataaaatatg ttgggcctga
    10621 attgtttttc aaactgctgg aatattttga ctccacctga tgccctgaat gaataaattg
    10681 cctgatcatc atccccaacg atgctagtgt taacacctgc cctcgtatgt agtgagatcc
    10741 aggcatactg aatcgagtcg gtatcctgaa cttcatctac aactaaatgt gtcaagttaa
    10801 gtggctgcat tttccccgag tacatctggc ccacaacaaa acgtgagagt gcattcaaat
    10861 ctgcaacgtg gtctttctga catattgcct gataggtgtt gaacaagtgg atttgttgcc
    10921 gcgtatggcg aactgaaatg atgtccgtat ccatttccct tccaatcgcg tcaatagcaa
    10981 attctgcatc cgggtatcga aaaggcgcgc cggactcctt caatgccctg tgaattacag
    11041 aacgctgtgc cgagctaatc aacatcttgc ccttccagtt gaccatatcc agttgcatcc
    11101 ggataaagcc gtggaatgta ttgaccagca ccctatcctg gtctttctta cttaatcgac
    11161 gtttcagttt ttcagccaat gaatttgttg ctgcacgggt aaatgtgacc atccccactt
    11221 tggcataagg gaactgcctg aggatagaga tcaccgcctc tatcatcgtg aatgactttc
    11281 cagatcccgg tgctgcaata accatattat tagcatttgc attttcaatg atgacccttt
    11341 gctcatctgt tggtttaccc ataaaagcct ctgaatattt agtccccttt attatacgat
    11401 ttaaatttga cggatttttc tttacaagac tcatttttgg tcacttcata t
//
LOCUS       DAFNBJ010000148        11367 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000148 DAFNBJ010000000
VERSION     DAFNBJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.148"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            32..784
                     /gene="gspC"
                     /locus_tag="L2X49_003856"
     CDS             32..784
                     /gene="gspC"
                     /locus_tag="L2X49_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HBN4798869.1"
                     /translation="MIIFISIGPIIFNVNYFHTTIVKNGQIINQPTNAFQSDFSLAAL
                     WRNENHAGVKDANPVAVNQETPKLSIALNGIVLTSNDETSFVLINEGSEQKRYSLNEA
                     LESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDIIKQPDSESQNVLADYIIATPI
                     RDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNLSLTHPDEVSQALSLLLTQQSA
                     QFTIRRNGVPRLINVSVGELTGMNGLRHERTQ"
     gene            768..2720
                     /gene="gspD"
                     /locus_tag="L2X49_003857"
     CDS             768..2720
                     /gene="gspD"
                     /locus_tag="L2X49_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HBN4798870.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNARKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYHSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHAPAPSSR"
     gene            2730..4211
                     /gene="gspE"
                     /locus_tag="L2X49_003858"
     CDS             2730..4211
                     /gene="gspE"
                     /locus_tag="L2X49_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HBN4798871.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVNLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFIYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLESEA"
     gene            4208..5404
                     /gene="gspF"
                     /locus_tag="L2X49_003859"
     CDS             4208..5404
                     /gene="gspF"
                     /locus_tag="L2X49_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HBN4798872.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5414..5851
                     /gene="gspG"
                     /locus_tag="L2X49_003860"
     CDS             5414..5851
                     /gene="gspG"
                     /locus_tag="L2X49_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HBN4798873.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     VVSDIVALENALDMYKLDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5859..6368
                     /gene="gspH"
                     /locus_tag="L2X49_003861"
     CDS             5859..6368
                     /gene="gspH"
                     /locus_tag="L2X49_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HBN4798874.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVTNTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6365..6742
                     /gene="gspI"
                     /locus_tag="L2X49_003862"
     CDS             6365..6742
                     /gene="gspI"
                     /locus_tag="L2X49_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HBN4798875.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRNAIERMRNET
                     LALWIADNQLQSQDSFGEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSIDNKSGQAQDD"
     gene            6735..7322
                     /gene="gspJ"
                     /locus_tag="L2X49_003863"
     CDS             6735..7322
                     /gene="gspJ"
                     /locus_tag="L2X49_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HBN4798876.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     QTQQYGGLQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7315..8298
                     /gene="gspK"
                     /locus_tag="L2X49_003864"
     CDS             7315..8298
                     /gene="gspK"
                     /locus_tag="L2X49_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HBN4798877.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTLSFHSQLQRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLEDGNTVKWQLRDAQH
                     CFNLNALAKISDDPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDSFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKRHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8313..9476
                     /gene="gspL"
                     /locus_tag="L2X49_003865"
     CDS             8313..9476
                     /gene="gspL"
                     /locus_tag="L2X49_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HBN4798878.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQKSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMLCYGVVPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGIFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRSIALWHTLKIEDQL
                     QQQQQETWQRYFPQIKRTHNFHFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9473..9934
                     /gene="gspM"
                     /locus_tag="L2X49_003866"
     CDS             9473..9934
                     /gene="gspM"
                     /locus_tag="L2X49_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HBN4798879.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQVTYGIVIEDLQFTLAGNEEITLRHLSFREQQ"
     gene            9934..10611
                     /gene="gspO"
                     /locus_tag="L2X49_003867"
     CDS             9934..10611
                     /gene="gspO"
                     /locus_tag="L2X49_003867"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HBN4798880.1"
                     /translation="MTMLLPLFILVGFIAGCFVNAIAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFVLTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10640..11116)
                     /gene="bfr"
                     /locus_tag="L2X49_003868"
     CDS             complement(10640..11116)
                     /gene="bfr"
                     /locus_tag="L2X49_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBN4798881.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11188..>11367)
                     /gene="bfd"
                     /locus_tag="L2X49_003869"
     CDS             complement(11188..>11367)
                     /gene="bfd"
                     /locus_tag="L2X49_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBN4798882.1"
                     /translation="CNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVRAAREV
                     MEDELMQLPEFKESA"
BASE COUNT         2968 a         2915 c         2726 g         2758 t
ORIGIN      
        1 gaatcataat aaagatgctg ccatcaatat attgattata tttataagca tagggccgat
       61 tatttttaat gtgaattatt tccatacaac tatagttaaa aatggacaga tcattaatca
      121 accaacaaac gcatttcagt ctgatttcag ccttgccgct ttatggcgta acgaaaatca
      181 tgctggggta aaagatgcaa atcccgtggc agttaatcag gaaacaccta agctctctat
      241 agcgcttaat ggcattgtac tcacatcgaa tgatgagact tcttttgtct taattaatga
      301 ggggagcgaa caaaagcgat actccctcaa cgaagccctt gagtcagcgc cgggaacgtt
      361 tattagaaaa ataaataaaa ccagcgttgt gtttgaaact cacgggcatt acgaaaaagt
      421 aactctccat ccagggctac ctgacatcat caagcagcct gattcagaaa gtcaaaatgt
      481 actcgctgat tatattatcg ccacgcctat acgtgatgga gaacagatat acgggcttcg
      541 attgaaccct cgtaagggcc tcaatgcatt taccaccagc ttgctacaac caggggatat
      601 cgccctcagg atcaataatc tttcgctgac ccaccctgat gaggtttcac aggcattaag
      661 tttactgcta actcaacaaa gtgctcagtt tacaattcgt cgcaacggcg taccccgctt
      721 gataaatgtt tccgtcgggg aacttacagg aatgaatgga ctgcgtcatg aaaggactca
      781 ataaaatcac ctgctgcttg ctggcagcac tactcatgcc ttgtgcagga cacgctgaga
      841 acgaacaata cggcgcgaac ttcaataacg ccgatatccg ccagttcgtg gaaatagtgg
      901 gtcagcatct tggcaaaacg atcctgatcg acccttcggt acagggaacc atttccgtac
      961 gcagtaatga tacgtttagc caacaggagt actaccagtt ctttttaagt attcttgatc
     1021 tttacggtta ttccgtgatc acgctggaca atggttttct gaaagtggtt cgctcagcta
     1081 atgtaaaaac atcgccaggg atgattgctg acagttctcg tccaggcgta ggtgatgagt
     1141 tggtcacccg aattgtaccg cttgagaacg ttcctgctcg tgacctggcc cccctgctcc
     1201 gccagatgat ggatgcgggt agcgtcggta atgttgtgca ttatgaaccc tccaacgttc
     1261 ttattctgac cggtcgtgcc tccaccatta ataaactgat tgaagtcata aagcgcgttg
     1321 atgtcatcgg cacagagaag cagcaaatta ttcatctgga atatgcgtca gcggaagatc
     1381 tcgccgagat tcttaatcaa ttaatcagcg aaagccacgg taaaagccag atgccagccc
     1441 tcctctccgc gaagattgtg gcggataagc gaaccaactc tcttatcatc agtggaccgg
     1501 aaaaagcacg ccagcgcatc acttcattac tgaaaagcct tgatgtcgaa gagagcgagg
     1561 aaggaaatac ccgggtttat tacctgaaat atgctaaagc cacgaatctg gtggaagtgc
     1621 taaccggtgt ttccgaaaag ctgaaagatg aaaaagggaa tgcgcgtaag ccctcctctt
     1681 ctggcgcgat ggataacgtc gccattaccg ccgatgaaca gactaactct ctggtcatta
     1741 ccgctgacca gtccgtccag gaaaaactcg ccacggtaat tgcgcgtctg gacattcgcc
     1801 gtgcacaggt gctggttgag gcaatcatcg ttgaagttca ggatggaaat ggactaaacc
     1861 tcggcgtgca atgggcgaat aaaaacgttg gcgcacagca atttaccaat accggattac
     1921 cgatttttaa cgctgcgcaa ggtgtggctg attataaaaa gaatggtggg atcaccagcg
     1981 cgaatcctgc ctgggatatg tttagcgcct acaatggcat ggccgcaggc ttcttcaatg
     2041 gcgactgggg agtactgctt accgcgctgg ccagtaacaa taaaaatgac atcctcgcca
     2101 ccccaagcat cgtaacgctg gataataaac tcgcgtcctt caacgtgggg caggatgtgc
     2161 cggtgctatc cgggtcacag accacttcag gggataacgt ctttaatacc gtcgaacgca
     2221 aaacggtggg gacaaaactc aaagttactc cgcaggtcaa tgaaggcgac gcggtgttgc
     2281 tcgaaataga gcaggaagtc tccagcgttg actcttcctc taactcgacg ctcggcccga
     2341 cgtttaatac ccgtactatt caaaacgccg tgctggtcaa aaccggtgaa acggtggtcc
     2401 tgggcggatt gctggatgat ttttctaaag agcaagtgtc aaaggttcct ctgcttggcg
     2461 atattccttt agtggggcaa ctcttccgct atacctccac cgagcgcgct aaacgcaacc
     2521 tgatggtatt tatccgtccg acgattatcc gtgacgatga tgtttatcac tcactgtcaa
     2581 aagagaaata cacccgttac cgtcaggagc aacaacagcg gatcgacggg aaatcaaaag
     2641 cgctggttgg ctcggaagat ttgccggtgc tggatgaaaa cacgttcaac agtcacgccc
     2701 ctgcgccatc gtcacggtga ggcattcata tgagaattca ctcaccgtac cccgccagtt
     2761 gggcgctggc acaacgaatt ggttatctct attcagaggg cgagattatt tatctcgccg
     2821 atacgccatt cgagcggtta ctcgatattc aacgtcaggt tggccagtgc cagaccatga
     2881 ccagcttgtc acaggctgat tttgaagctc ggctggaagc ggtattccat cagaataccg
     2941 gtgagtcgca acagattgcg caggatatcg atcaatccgt caatcttctc tcgctttcgg
     3001 aagagatgcc cgcaaatgaa gatctcctga atgaagattc agcggcaccg gttatccgct
     3061 tgatcaatgc gattttgagt gaggccatca aagaaaccgc ctctgatatc cacattgaaa
     3121 cctatgaaaa aacaatgtcg atccgttttc gcatcgacgg cgttttgcgg acaattttac
     3181 agccaaacaa aaaactggcg gcactgctta tctcccgaat taaggtcatg gctcgtcttg
     3241 atatcgccga aaaacgtatt ccacaggatg gaagaattag tttgcgtatc gggcgacgta
     3301 acatagatgt ccgcgtatcc acactgccgt ccatctatgg tgaacgcgcc gtactccgcc
     3361 tgctggataa aaacagcctc cagctttcat tgaacaacct ggggatgacg gcagcggata
     3421 agcaggattt agaaaatctc attcagcttc cgcacggtat tatcctggtg acagggccga
     3481 caggctccgg taaaagcacc acgctctacg ccatcctttc ggcgctgaat actcccggcc
     3541 gcaatattct gacggtagaa gatcccgtgg aatatgagct ggaaggcatt gggcaaacgc
     3601 aggtgaatac ccgtgtggat atgtctttcg ctcgcggcct gcgcgccata cttcgccagg
     3661 acccggatgt cgtcatggtg ggggaaattc gtgatacaga aaccgcgcag attgcggttc
     3721 aggcctcgct caccggccat ctggtactct caacactcca cactaacagt gcatcaggcg
     3781 cagtgacccg gctccgcgac atgggcgtcg aatcattcct gctttcgtct tccctggcag
     3841 ggattatcgc gcaacgtctg gttcgtcgcc tgtgtccgca atgccgacaa ttcacgcccg
     3901 tatcacccca acaagcgcag atgtttatat atcatcagct cgcggtgaca acaattggca
     3961 ctcccgtagg ctgccctcat tgccatcaat ccggctatca ggggcgcatg gcgatccacg
     4021 aaatgatggt ggtgacgccg gaattacggg ccgctattca tgaaaatgtg gatgaacaag
     4081 cactggagcg actagtccgg caacaacaca atgccttaat caaaaatggc ctgcaaaaag
     4141 tgataagcgg tgacacctcc tgggatgagg ttatgcgcgt cgccagtgcc acgctggaga
     4201 gcgaagcatg aattatcgct atcgcgccat gacccaggat ggtcaaaaat tgcaagggat
     4261 cattgatgct aacgatgaac gtcaggcacg actgcggctg cgtgaagaag ggcttttcct
     4321 gctggatatt cgcccccaaa aaagttcggg agtaaaaaca cgtcgcccga ggatcagcca
     4381 tagtgaactg acgcttttca cccggcagtt ggcaacctta agcgcagcgg cattacccct
     4441 ggaagagagc cttgccgtaa tcggtcaaca aagcagtaat aaacgactgg gtgacgtgtt
     4501 aaatcaggta cgcagcgcca tccttgaagg gcatcccctt tccgatgcat tacagcattt
     4561 tcccacgctt ttcgattcgc tctatcgtac cctggtaaaa gcgggcgaaa agagcgggct
     4621 gctggccccg gtgttggaaa agctggctga ttacaatgaa aaccggcaga aaatccgcag
     4681 caagctcatt cagtcactga tctacccctg tatgctcact acggtggcga ttggggtcgt
     4741 gattattctc ctcactgctg tcgtgcccaa aattaccgaa cagttcgtgc atatgaagca
     4801 gcaactgccg ctgagtacac gcattctttt aggtctgagc gacacgttgc aacgtaccgg
     4861 cccgacatta ttagcgacag tgtttattgt cgctgtaggt ttctggctct ggttaaaacg
     4921 cggcaataac cgccaccgtt ttcatgccat gttgctgcgc gttgcgctca tcggcccgct
     4981 gatttgcgcc attaacagcg cacgctatct ccgcacttta agtattttgc aatccagcgg
     5041 cgtccctctg ctggatggga tgaatttgtc caccgaaagc ctcaacaacc ttgaaattcg
     5101 ccagcgtctg gcaaatgcgg cagagaacgt tcgccagggt aacagcattc atctttcgct
     5161 ggaacaaacc gcaattttcc cgccgatgat gctctacatg gtggcctctg gcgaaaaaag
     5221 cgggcagctc ggcacattaa tggtcagagc cgcagataac caggagacac tccaacaaaa
     5281 tcggatcgcc ttaacgctct ccatcttcga gccagcactc attattacga tggcactgat
     5341 cgtcctgttt attgtcgtgt cggtactcca acctcttctt caacttaact caatgattaa
     5401 ttaaggaaag catatgcgcg caacggataa gcaacgcggt tttacattac tggaaattat
     5461 ggtggtgatc gtcattatcg gcgtacttgc cagcctggtg gttcctaacc taatgggcaa
     5521 taaagaaaag gcggataagc aaaaagtcgt cagcgatatt gtggcgctgg aaaacgccct
     5581 tgatatgtac aaactcgaca atcatcacta tccaaccaca aatcaggggc ttgaatcctt
     5641 agtagaagcg ccgacactgc caccgctggc cgcaaactat aacaaggaag gttatatcaa
     5701 gcgtctgcct gccgatccct ggggcaatga ttatgtcctc gttaatcctg gtgaacatgg
     5761 tgcatacgat ctgctttcag cagggcccga tggtgaaatg ggaaccgagg acgacatcac
     5821 caactggggt ttgagcaaga agaaaaagta agtcagcgat gaatcagcaa cgcgggttta
     5881 ccctgctgga gatgatgctg gtgctggcgc tggtcgctat cacggcaagc gtggtgctct
     5941 tcacatatgg ccgggaggat gtcaccaaca ctcgtgcgcg cgaaacagcg gcgcggttta
     6001 ccgctgcact tgaattagcc atcgaccgcg ccacgctcag tggtcagcct gtcggtatcc
     6061 atttctctga ttcagcatgg cgcattatgg taccgggcaa aacaccatca gcctggcgct
     6121 gggttccatt acaagaagat gccgcagacg agagccaaaa tgactgggat gaagaactct
     6181 caattcatct tcagccattt aaacctgatg acagcaacca gccacaggtt gtgattctgg
     6241 ccgacggaca aatcacgccc ttctctttgt tgatggctaa cgccgggacc ggtgagccgc
     6301 tcctgacgtt agtctgttca ggctcatggc ccctggatca aacgctcgcc agagataccc
     6361 gcccatgaac aaacaatcag ggatgacact gcttgaagtc ttactggcga tgagtatttt
     6421 caccgctgtt gctctgactt taatgagcag tatgcaaggg caacgaaatg ccatcgagcg
     6481 aatgcgtaac gagacgctgg cattgtggat agcggataac cagcttcagt cacaagattc
     6541 attcggcgag gaaaacacgt ccagttctgg caaagagtta ataaatggcg aagagtggaa
     6601 ctggcgcagc gatatccact caagcaaaga cggcacgctt ctggaacgca ccataacggt
     6661 gacattaccc agcggtcaga caacctcgct tacgcgttat caaagcatcg ataataaatc
     6721 cgggcaggca caagatgatt aatcgccagc agggtttcac attgctggag gtcatggcgg
     6781 cgctggcgat tttctcaatg cttagcgtgc tggcgtttat gattttttcg caagcttccg
     6841 agttgcatca acgctcacag aaagaaatcc agcagttcaa tcagctacag cgcaccatca
     6901 ccattcttga taacgatctc ctgcaactgg tcgcccggag gaatcggagt acggacaaaa
     6961 tcatggtgtt gggtgaagaa gctattttta ccacgcaaag tcgcgatccg ctggctcctc
     7021 tcagtgaagc acaaacctta ctcactgttc actggtatct gcgaaatcat acgctctacc
     7081 gtgctgttcg tacttctgtg gatggcagga aggatcaacc cgcccaggcg atgctggaac
     7141 atgtcgagag ctttcttctg gaaagtaaca gcggggaaag ccaggaactt cctctctcag
     7201 tcacgttaca tctgcagact cagcaatatg gggggttaca gcgccgtttt gcattacctg
     7261 agcagttagc gagagaagag tcgccagctc agacgcaggc cgggaataat aaccatgaat
     7321 aatgaacaac gcggcgtcgc gctgctcatt gtactcatgt tactggcgct catggctgct
     7381 ctggcagcag acatgacgct cagttttcac agccagcttc agcgaacccg ccaggtaaac
     7441 catcatctgc aacggcaata tgatattgaa ctggcggaga aacttgcgct tgccagcctc
     7501 acccaggacg ttaaagataa cgacagacag accacgttgc agcagtactg ggcacagcca
     7561 caacagttgc agctggaaga tggcaataca gtgaagtggc agttacgcga tgcccagcac
     7621 tgttttaatc tcaatgcgtt agcaaaaatc tctgacgacc cgctggcttc ccctgatttc
     7681 cccgcccagg tattcagtgc gctgctgatt aacgccggta tcgatcgcgg aaacaccgat
     7741 gagatcgtgc aatcaatcgc tgactatatt gatgtcgatg actcaccacg ctttcacggc
     7801 gcagaagata gcttttacca aagccagacg ccgccccggc atagtgccaa tcagatgctt
     7861 tttctgactg gcgaattacg tcagataaaa ggcataaccg aaaacatcta tcagcggctt
     7921 atcccgtatg tctgcgttct cccgaccacg gaacttagca tcaatcttaa tatgctaacg
     7981 gagaacgata ttccgctctt cagagcgtta ttcctcaata acatcacgga tgctgacgct
     8041 cgggtcttat tgcaaaaaag gccgagggaa ggctggctaa ccacggatgc atttctttac
     8101 tgggcgcagc aggatttctc cggtgtgaag ccactggttg ctcaggtgaa aaggcatctc
     8161 tttccctata gccgctattt tacgctgagt acggaaagta tcagcgacga acaatcccag
     8221 ggatggcaaa gccatatttt tttcaaccgt aaacagcaga gcgcccaaat ctatcggcgt
     8281 acgctgcaac tctactaatc ataaggatcg tgatgcctga atcgttgatg gtcatacgtt
     8341 cttcctcgac gctgcgtaag cattgggaat ggatgacgtt ctccgcagat agcgtatctt
     8401 cggtacatac gctcacggac gatcttccgc tggagtcgct ggccgatcag cccggtgccg
     8461 gaaatgttca tcttttgatc ccaccagagg gccttttata tcgttccctc acgctgccca
     8521 acgccaaata caaactcacc gcacaaacat tacaatggct ggcagaggag acgttacccg
     8581 acaacaccca ggactggcac tggacggttg tcgataaaca aaacgaaagt gtcgaggtga
     8641 taggcattca gtcggagaaa ctcagtcgct atcttgagcg tttacatact gccggactca
     8701 acgtgacgcg ggttttgcca gatggctgct atcttccctg ggaagtagat agctggactc
     8761 tggttaacca gcaaaaaagc tggctcattc gcagtgccgc acatgccttc aacgaactgg
     8821 atgaacactg gttacagcat cttgctgccc agtttccgcc tgagaatatg ctttgttatg
     8881 gtgtagtacc tcacggcgtt gcagcggcca atccacttat ccagcatcct gaaataccat
     8941 cactgagttt gtattctgcc gatattgcat tccagcgcta tgacatgctg catggtattt
     9001 ttcgtaaaca gaaaactgta agcaaatcgg gaaaatggct cgcccgcctg gcagtaagct
     9061 gtctcgttct cgccattctt tcttttgttg gcagccggag catcgcgctc tggcatacgc
     9121 ttaagattga agatcaactt cagcaacaac agcaagaaac ctggcaacgt tatttcccgc
     9181 agatcaaacg cacccacaac tttcattttt actttaagca gcaactcgct cagcaatatc
     9241 ctgaagctgt accgttgctc tatcatttac aaacacttct gctggaacac cctgaactgc
     9301 agttaatgga ggcgaactat agccagaagc aaaagtcgct gaccttaaaa atgagtgcta
     9361 agagtgaagc caatatcgat cgtttctgtg agttaacaca gtcctggctt ccgatggaaa
     9421 aaacagaaaa agatccggtc agtggtgtat ggacagtaag gaactcaggg aaatgattaa
     9481 atcatggtgg gcagaaaaat ccacatcgga aaaacagatc gttgcggcac tggcggttct
     9541 ctcgctcggc gttttttgtt ggcttggggt gataaaaccg atagatacct acattgcaga
     9601 gcatcagtcc cacgcgcaaa aaattaagaa ggacatcaaa tggatgcagg atcaagcgag
     9661 cacccatggt ttactcggtc atcctgcgct tacgcagccc atcaaaaata tccttctcga
     9721 ggaagcaaaa cgtgagaatt tggctatcac gctggaaaac ggcccggaca atactctgac
     9781 aattcatcct gtcacagctc ccctcgaaaa cgtctcccgc tggctcacta cggcacaggt
     9841 aacatacggt attgttattg aagaccttca gtttactctt gccggaaacg aagagatcac
     9901 cctcaggcat ttgtccttca gggagcaaca ataatgacaa tgctactgcc gctcttcatt
     9961 ctggttggtt ttattgcagg ttgttttgtt aatgccatcg cctatcacct ctcgccgttg
    10021 gaggataaaa cggcgttaac gtttcgccag gtattggttc atttcaggca aaaaaaatat
    10081 gcctggcatg atacagtgcc cctgatactt tgtgttgctg ctgctatcgc ctgcgccctg
    10141 gcacctttca cgcccatcgt gactggcgca ctctttctct atttctgttt cgtactcacg
    10201 ctcagtgtta ttgattttcg cactcagctc ctgcccgaca aactcacctt accgctgctc
    10261 tggcttggct tggtatttaa tgcgcagtat ggattaattg atttacatga tgcggtttac
    10321 ggcgcggtag cgggctatgg ggtgctgtgg tgtgtttact ggggcgtctg gttagtttgt
    10381 cacaaagagg gattgggcta cggtgatttc aagctactgg ccgctgcagg cgcatggtgt
    10441 ggctggcaaa cgttgccaat gatactgctg attgcctcgc tgggtggcat tggttacgcc
    10501 atcgtttcac aacttctgca acgccggact ataaccacta tcgcattcgg cccgtggctt
    10561 gcgctcggca gcatgataaa cctggggtat ctggcctgga tctcttatta accgggaggg
    10621 ttctccctcc cgacacggct caaccttctt cgcggatctg tgcttgcaga taattttgca
    10681 ggcccatctt ctgaatcaga tcaagttccg tttccagcca gtcgatatgg ccttcttcat
    10741 cacgcaaaat ttctatcatc atatcgcggc tgacgtaatc atgaacgcta tcggcataac
    10801 caattgcctc acgcaaattc ttcgcgccat ccagctcaag tgccagatca gaacgcagca
    10861 tttcctcaac atcttcacca atgttcagtt tgcccaggtc ctgtaagttt ggaagacctt
    10921 ccagaaaaag aatgcgctca atataacgat cggcgtgttt catctcatca atggattcat
    10981 gatactccac atcattgaga cgtttgagac cccagttttt aaacattcgg gcatggagaa
    11041 agtactgatt gattgcgaca agctcatttc ccaacagttt gttgagataa tttataactt
    11101 tagtatcacc tttcattttt tgactccttc gcttccactc tttgaagagt agaacggctt
    11161 acgagtaagt caaaaaacac actccgttta tgcggactcc ttaaactccg gcagctgcat
    11221 taattcatcc tccatcactt cacgcgcggc ccggacacat ttaccgcact gattgccgac
    11281 cggaataaat ttttttaatt gctggaacga gtgaggggaa aactggcgca cagcctgacg
    11341 aattttttta tcgctgatac cattaca
//
LOCUS       DAFNBJ010000149        11319 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000149 DAFNBJ010000000
VERSION     DAFNBJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.149"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            245..826
                     /gene="mdaB"
                     /locus_tag="L2X49_003870"
     CDS             245..826
                     /gene="mdaB"
                     /locus_tag="L2X49_003870"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBN4798883.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            857..1171
                     /locus_tag="L2X49_003871"
     CDS             857..1171
                     /locus_tag="L2X49_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBN4798884.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1219..3111)
                     /gene="parE"
                     /locus_tag="L2X49_003872"
     CDS             complement(1219..3111)
                     /gene="parE"
                     /locus_tag="L2X49_003872"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBN4798885.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLAAQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3140..3721)
                     /gene="yqiA"
                     /locus_tag="L2X49_003873"
     CDS             complement(3140..3721)
                     /gene="yqiA"
                     /locus_tag="L2X49_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBN4798886.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3721..4548)
                     /gene="cpdA"
                     /locus_tag="L2X49_003874"
     CDS             complement(3721..4548)
                     /gene="cpdA"
                     /locus_tag="L2X49_003874"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBN4798887.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4573..4995)
                     /locus_tag="L2X49_003875"
     CDS             complement(4573..4995)
                     /locus_tag="L2X49_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBN4798888.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(4996..5625)
                     /gene="nudF"
                     /locus_tag="L2X49_003876"
     CDS             complement(4996..5625)
                     /gene="nudF"
                     /locus_tag="L2X49_003876"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBN4798889.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5830..7311
                     /gene="tolC"
                     /locus_tag="L2X49_003877"
     CDS             5830..7311
                     /gene="tolC"
                     /locus_tag="L2X49_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBN4798890.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7459..8130
                     /locus_tag="L2X49_003878"
     CDS             7459..8130
                     /locus_tag="L2X49_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBN4798891.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8136..9296
                     /locus_tag="L2X49_003879"
     CDS             8136..9296
                     /locus_tag="L2X49_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBN4798892.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9334..10122)
                     /gene="ygiD"
                     /locus_tag="L2X49_003880"
     CDS             complement(9334..10122)
                     /gene="ygiD"
                     /locus_tag="L2X49_003880"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBN4798893.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10265..11038
                     /gene="zupT"
                     /locus_tag="L2X49_003881"
     CDS             10265..11038
                     /gene="zupT"
                     /locus_tag="L2X49_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBN4798894.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAVVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11096..11266)
                     /locus_tag="L2X49_003882"
     CDS             complement(11096..11266)
                     /locus_tag="L2X49_003882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798895.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         2789 a         3047 c         2807 g         2676 t
ORIGIN      
        1 ttctccatga ttattttttc cgaaaagctc ctgtatcatt tgcataatac atctctcctg
       61 tgggaactta tgggaagttg atttcagtat tgtcattttc agaaaatatt tatcctgcat
      121 cggtgagtca gagcaagatc agacttttgc taaattcgca aaagactttg cacattttgc
      181 taatttcacc gtaccgctct gtgacgtact atagtcggca aacgtctcac cttgaggtta
      241 aaaaatgagc aacatcctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca
      301 actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt
      361 ccgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa actttctctg
      421 ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt ggacagtgaa
      481 aaaatatatt gatgatgtat tcaccgaagg tcatggaacg ctgtatgcca gcgatggccg
      541 tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg gcaaaaaata
      601 tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag atcaattctt
      661 ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc aattccttgg
      721 catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg
      781 ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag agctcaggct
      841 ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc ctggtcaaca
      901 tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga
      961 aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat
     1021 ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc ttgaagcgca
     1081 tctgcaaacc ccgcacatga aggcgtatag cgaagccgta aaaggcgacg tgctggagat
     1141 gaatatccgt attctgcagc cagggattta atcctgcctt gtttgcccgg ccatcctgac
     1201 cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct cttgcaacca
     1261 gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg cgtcagtacg
     1321 ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg gatcaagcgt
     1381 ggtttcgcgc aattgcatcg ggttcatttc cccgagacct ttaaaacgct ggacgttcgg
     1441 cttgcctttc ttgcgtttta attgctcaag tacgccttct ttctcttctt ccgtcagcgc
     1501 gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga cgtaaacgtg
     1561 accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca gcgtggcaat
     1621 gtgcagacca tcggagtcag catccgcgag gatacagatt ttgccgtaac gaagctggct
     1681 caaatcgtcg ctgtcaggat cgataccgat cgctaccgaa atatcatgca cttcctgcgc
     1741 ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt tcagtggcat
     1801 gatcgcctga tattcgcgat cgcgcgcctg cttggcagat ccgcctgcag agtcaccttc
     1861 cacaaggaac agttcggtac ggttaagatc ctgcgcggta cagtcagcca gtttgccagg
     1921 cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac gcatacgacg
     1981 ctgggcgctg gaaatcgcca tctccgccag cagctccgcc gcctgaacgt tctggttcag
     2041 ccacaggatg aaggcatctt tcaccacgcc cgaaacgaat gccgcacact gacgcgaaga
     2101 gagacgctct ttggtctgcc cggcaaactg cggatcctgc atttttactg acagcacata
     2161 ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca gaatgttgcg
     2221 atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat taacatgggt
     2281 accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt caccgccttc
     2341 cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat taccgataaa
     2401 cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat cattcagacc
     2461 gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg taatctcaac
     2521 gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa cagaaaatcg
     2581 cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccag tattgcgttt
     2641 accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa aggcgatgtt
     2701 ataaacctga ccatcgcggc gcacgttaac ttctacgcgc ttcgacaggg cgttaaccac
     2761 cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag agaatttacc
     2821 gcctgcatgc agacggcaaa gaatcagttc aaccgccggt acaccctctt ccgggtgaat
     2881 atccaccggc atcccgcgcc catcgtcaat cacttctaac gactggtcag catgtaagat
     2941 tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat caataacttc
     3001 ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc ggcgaaccgg
     3061 ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca tggtttaagt
     3121 tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg atcgggttga
     3181 aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc tggcggcagg
     3241 aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc tgttgcagca
     3301 gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga tcgtaaatat
     3361 ggcgtgactc tagcacatat tgctgcccgg tgtaggggtt ctcgttctga ccgagatagt
     3421 ccgtcagcag ttcaaacggg cgtaccgccg ggtttaccac cactgcgggc agcataaaac
     3481 attgcgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata cccagcgaat
     3541 cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa ggatacggcg
     3601 gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag ttttttaaca
     3661 agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca
     3721 tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc gatgcacctc
     3781 ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg cgatggtgtc
     3841 cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag
     3901 gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca gcaagtattt
     3961 gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca gactgtgttg
     4021 atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca acgtatggcg
     4081 ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc tcagctcacc
     4141 gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct caccaataaa
     4201 cacgcgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa
     4261 atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa
     4321 atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga caatcaggtc
     4381 gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt
     4441 tacccctaac agggcttcgt gcttttgtgc aaacaggtga gtatcggtaa tttgtaaaat
     4501 cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat ggtgtcctta
     4561 ggtttcacga cgctaataaa ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa
     4621 ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca actttttatt
     4681 aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca cttcagccac
     4741 catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg
     4801 cgcagtctgt tctattgtca ctagggtagt gtatcgggtc gattccacaa tcgtcagccg
     4861 atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa
     4921 caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag gaaagtcagg
     4981 tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca gctgcagcca
     5041 ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc actggtatgc
     5101 ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca gaccatgaat
     5161 accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct cactggtgcc
     5221 tcccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca gtcccgcctc
     5281 ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcaa tcatcccggc
     5341 aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc gaatctgctc
     5401 aatcagcaca acttcatcac gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg
     5461 ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaataaac gatgacgaaa
     5521 tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac
     5581 atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta atgtcctggc
     5641 actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga gcagtcatgt
     5701 gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc
     5761 ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa
     5821 ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc tgggttcagt
     5881 tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg
     5941 gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc
     6001 agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc
     6061 gacgcgaacg gcatcaactc taacgcgacc agtgcgtcct tgcagttaac tcaatccatt
     6121 tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg gattcaggac
     6181 gtcacgtatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac
     6241 gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc gatctaccgt
     6301 caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac cgacgtgcag
     6361 aacgcccgcg cacagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt
     6421 gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga actggctgcg
     6481 ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa
     6541 gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct ggcgcgcgag
     6601 caaattcgcc aggcgcagga tggtcactta ccgactctgg atttaacggc ttctaccggg
     6661 atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac
     6721 gatagcaata tgggccagaa caaagttggc ctgagcttct cgctgccgat ttatcagggc
     6781 ggaatggtta actcgcaggt gaaacaggca cagtacaact ttgtcggtgc cagcgagcaa
     6841 ctggaaagtg cccatcgtag cgtcgtgcag accgtgcgtt cctccttcaa caacattaat
     6901 gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca aagctcatta
     6961 gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt gttggatgcg
     7021 accaccacgt tgtacaacgc caagcaagag ctggcgaatg cgcgttataa ctacctgatt
     7081 aatcagctga atattaagtc agccctgggt acgttgaacg agcaggatct gctggcactg
     7141 aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcccc gcaaacgccg
     7201 gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcccccgt cgttcagcaa
     7261 acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg atgacgacga
     7321 cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt
     7381 cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac
     7441 tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa
     7501 aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg
     7561 gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca
     7621 gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa
     7681 gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa
     7741 ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca ggcgcaggcc
     7801 cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat ggggcgtctg
     7861 atgggcggtg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa cccagccagt
     7921 ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc
     7981 cgcaccatga ccgtaccgaa gacggcaatg gcaccaaaac cggcgaccac cactaccgtt
     8041 acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagtgcaacc
     8101 ggtacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc
     8161 gcccggactg gcgtgagaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg
     8221 agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt gaaaagctgg
     8281 aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca
     8341 gcgatgagct gatgaccaaa ttccgcattc caaaacacac ctggagtttt gtgcgccagt
     8401 catggctgac gcaccagcca tcgctttatt cgcgtcttga tctggcgtgg gatggcactg
     8461 gtgaacctaa acttctggaa aataacgccg atacgccaac gtcactatac gaggcggcgt
     8521 tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg
     8581 accagtttaa cagtctgcaa gaaaaactga tcgatcgctt cgttgagctg cgtgaacagt
     8641 atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca
     8701 ttcagtattt gcaggactgc gcaacggaag ctgaaattgc tactgagttc ctctacatcg
     8761 atgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag gtaatttcca
     8821 acctgttcaa actgtatccg tgggaattta tgttgcgtga gatgttctca accaagctgg
     8881 aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaggcac
     8941 ttctaccgct actgtgggag atgttcccga atcacccgaa cctgctgccc gcttattttg
     9001 cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc tcccgtgaag
     9061 gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg
     9121 gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata
     9181 tgctgattgg tagctggctg gtgaacgatc aacccgccgg aattggcatt cgtgaagacc
     9241 gtgcattgat cacccaggat atgtctcggt tttatccaca tatttttgtt gaataagcca
     9301 cgataccgga tggcactcgc catccggtaa ttgttagcct atctgcaccg acagcatact
     9361 taaactgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc catcccacgc
     9421 acctaacaca tacaacaacg gcagatagtg ctctggcgtt gggttcgata acgcgccacc
     9481 ttcatggtcg agataattca ccagaggatg ctgctctacc ggcccttgcc acgtcagatt
     9541 cgctttcaca tactcattaa acgacgtcgc ccacggatac ggtgaactat caccgtgcca
     9601 cttcactgtg cgcaggttat gcaccacgtt gccactggcg accaacatta ttccttcatc
     9661 tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc
     9721 gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca gcacgcccca
     9781 ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg gcgctaacag
     9841 ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg tatcgtacag
     9901 cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg cggtcactcc
     9961 tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc gtggcaatgt
    10021 catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact
    10081 accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa
    10141 aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca
    10201 atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt ataaggagtt
    10261 gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc
    10321 gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt cgctaggatt
    10381 tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg cactagcagc
    10441 tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc ttggctattt
    10501 tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa aatcggtgca
    10561 gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca tcagtctgca
    10621 taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg agctgggatt
    10681 tggcatcgca ctggccgtcg cgttgcacaa tattcctgaa ggtctggcgg ttgcaggccc
    10741 ggtttatgca gcaacgggtt ctaaacgcac ggcaattctg tgggcgggga tttctggatt
    10801 ggcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga tttccccggt
    10861 ggtcatggcg gcaatcatgg cggtggttgc aggaattatg gtggcgctct cggttgatga
    10921 attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg
    10981 tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa
    11041 acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt
    11101 ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca
    11161 gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca
    11221 ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt ttacccttaa
    11281 tatttaacaa tactaaacat ttagcgcata aatttcaca
//
LOCUS       DAFNBJ010000150        11254 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000150 DAFNBJ010000000
VERSION     DAFNBJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.150"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(132..932)
                     /locus_tag="L2X49_003883"
     CDS             complement(132..932)
                     /locus_tag="L2X49_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trhR"
                     /protein_id="HBN4798896.1"
                     /translation="MSESDNITRNLLGKLLRTEISIIRSYRAFLMLLPLHGSSKYQTG
                     SPLLQRRLFGNGFGAMIDNAFEVETRPGSFLVPRSLSKEISWDKFFVAVVDGDTNVIR
                     EYDSEDTDFGIYNEGEKVTLLSGQEEFYNPRKIQQLRSKCVDIQNDYLMQVFFMSMLA
                     PEFVSIFFGLKPATVEAIKDVGMSSLKLINDVVLFPRTIPFTPGLGDDSATLKSKVFA
                     WAYEISADIRLGKISDDLMELLRYDTMFTCHRQDVFNTLANKVLLKDY"
     gene            1690..2175
                     /locus_tag="L2X49_003884"
     CDS             1690..2175
                     /locus_tag="L2X49_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000045622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HBN4798897.1"
                     /translation="MSKSKLLKSLDISRTLLRSQIDVLREEAEELITNYWIEWKERNH
                     IERNLPRDSKTLKKEYLGSYAPKVELIGNARKVTITWHQFSPYKNRPPSHMSKRISPM
                     KSGKYTKSCFVNHASWEYEMIEKTEGLLEPYRELLEFYHAAYIELGRKVRQYSKSEVA
                     Q"
     gene            2172..2894
                     /locus_tag="L2X49_003885"
     CDS             2172..2894
                     /locus_tag="L2X49_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798898.1"
                     /translation="MTDVTMNESEHKVPDVNTRSPRASDNSRRVVQSKNEHYGMATVT
                     ITPGTPDFNRFLTARNRSVIRGFDDVSIAISSLFRTVNAVKHPELVQAIQDWFNELHE
                     ENNLMKNNLERHIATIHIDESDPFFSSTEFSPFRFEPVQLNFNNQNTMRFYKHIFEMN
                     NLLTQMHKFNSLGQLPVSDYNVMAHNIIRSLNMYIERVKKTLNVSRRAKGTYSPDEFI
                     EKVKQYKSVQAYIAAELSGKRR"
     gene            2905..5940
                     /locus_tag="L2X49_003886"
     CDS             2905..5940
                     /locus_tag="L2X49_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HBN4798899.1"
                     /translation="MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGE
                     QKYTVPDHPNPEVLKFIEYPTRPAGIQTFNEQSILSLYRDKLHSISMMLAISDGDIRE
                     DAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLLSHSLEVAMISLKNANHSELR
                     PIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPSSQSLLDWA
                     RENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNV
                     YNDGNDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININN
                     YKSKPMHIIIVNGEVYLNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVQRGYVE
                     PYDDERVVHYFIPGTYSENDIASIFRNGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPG
                     LFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVNDAIENTPQYGLQLVNGPD
                     ADSNNIISSENTEAITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQVEEHDD
                     RSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAAIALIPGIDEAEFCNGPFFQL
                     TYRDGSLDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDI
                     PEAPSVNEAASPPQTPLELPRPRLNAPVAEEASSVAEQTNAHSEPESVIATEYEQYGH
                     LLEETLESDGEAYSDLIASDSTEAEYPATDPQSSDFAQLPGETALSVAPGDLDYSEGA
                     IKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALARLFAVSTHAEKKHEKT
                     QEPSPVKEVKNPTSSTTVKAPIPIEPPGAEEKEAVEEFTLLNDGEVTELEYVEIATML
                     HQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSG
                     AVVNTKCIAFNIPTLVAASDRAKVDIELIINKLKEVGNL"
     gene            5940..8024
                     /gene="traD"
                     /locus_tag="L2X49_003887"
     CDS             5940..8024
                     /gene="traD"
                     /locus_tag="L2X49_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284076.1"
                     /note="Members of this protein family are the putative
                     conjugative coupling factor, TraD, as the term is used for
                     the SXT and TOL plasmid systems; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer system coupling protein
                     TraD"
                     /protein_id="HBN4798900.1"
                     /translation="MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDG
                     VNIAPIILYTAVFLLLVCLYRCKTAHPYLMAHWRVFQRQIMFISLKSLRTINKSNFFS
                     NERKYRQLVQEYKKTNRPVPDRKTYFCNGFEWGPEHADRAYQIANLSSDKREIALPFV
                     LSPIARHFETMARSMGGNNAIFAVDRRAPIFVTEDNWFGHTLITGNVGTGKTVLQRLL
                     SISMLHLGHVIVVIDPKNDAEWRQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYT
                     NVSDLTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMS
                     VVNLTIKVMECCFARHYGPDVWMEKVKYASNDTLQVRFKRLTEWFNAHFLNYEGAEPI
                     EWIDTVGRLVDYSMSDPEHMSKMTAGIMPLFSRLTEQPLNELLSPSPNTLTSREIVTS
                     DGMFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGSRYNADDGDMSSHSRISIF
                     VDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANAETANRITGLCNNYISL
                     RVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPK
                     ELLGQVPKFHIVARLQDGRKVVGQIPIAVSEKAMKPNTTLLEMFLKSAGKVTLRQNVG
                     LSYLNKYLRKLH"
     gene            8024..9127
                     /locus_tag="L2X49_003888"
     CDS             8024..9127
                     /locus_tag="L2X49_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798901.1"
                     /translation="MDEQITLTQNQIFSASLKVSKSRSLVKRRMQSLGLKFTESQDVR
                     NRLAGIEKGALKCVGQFCHDNDAESLSAMAIILSELFLLQGELSTSNEYGDHETSYWT
                     VAQGPCDEWVQSLLASENGRRTFNSFRITFDNSEERRSLVEKNAKMLGSYLLPYFVNF
                     TNAASAFITLPNSITFKQVQRNKPLIHPETTLSHILTIEDSAFLSRIKFKLISAIDRL
                     PDPSGQYANMFNHIMDRALLTHLNREQIDSPCVCKKVISTYADTMLTLPIFNTTITGK
                     YRHWTPWGINFVEFSRQAAKEKSCVYVPEPGQIHWKSPEHKELAEYSLINQIIPQQYH
                     WLLGVPTIWRSHYRDHSKRLDLFKEWRDANGCG"
     gene            9114..9776
                     /locus_tag="L2X49_003889"
     CDS             9114..9776
                     /locus_tag="L2X49_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HBN4798902.1"
                     /translation="MAADNSARAVFIRGLLLLGMMLYLIYSLGFQNTDELKQQITQEV
                     NASRSLISNDRWLSVIANSEATLSTLINDYKLIDYLNTILIPDTSTPARGMNYVAEKM
                     SSVNYTMAKNLPLLIYQSIFRWNLILGWLIVFSPYLIAMLADGMYQWKLKRYVFGNVT
                     VQFYRIWFRAFWIIGALTFIYLSMPNMSLFNNIAQLFPPVALLILGIALNRLWSNFQK
                     LM"
     gene            9789..10970
                     /locus_tag="L2X49_003890"
     CDS             9789..10970
                     /locus_tag="L2X49_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBN4798903.1"
                     /translation="MFKRNNKKSPANIKESLDENADRFYKMFRIHTTSKVVISLLALA
                     AAGVSAFNIYDKYQESEGRMDHIAVVRISGEMGTGSEVGDGSVIATALAKAYNNPHAK
                     AVIIEAESGGGGPSDAIIIYRQINALRSHQQKIERVSHAESTSKPRNTSDHSASRSQA
                     DSNAELSKRNTLDVLSAGSGHFISDMENSYKPIIVSVKGICASACYYAVSPADAIYAD
                     SSALTGSIGVRMDHWNLSRVMDTVGVKNEPLTAGEFKDALDPYHPLSDATRDFMQKQI
                     LDAMHEQFIADVEQGRGKKLLSRPEADAVALYSGRVWTTQQAIRYGLIDGDLTPVEVR
                     SRLSQMYSTTIFKTYNEPQRSLRSALGMLMSLSTNVETLAGTTSRILDSVQATSYPTV
                     R"
     gene            10972..>11254
                     /locus_tag="L2X49_003891"
     CDS             10972..>11254
                     /locus_tag="L2X49_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HBN4798904.1"
                     /translation="MDAILMVWRVIGISIIVLVLIVLYCLMGFVSSAIAERRTARQIE
                     IYLPDDAEGLLSDLTLPVNATGTTQIDHVLIASHGLYVIEQKNYAGKLYG"
BASE COUNT         3269 a         2518 c         2476 g         2991 t
ORIGIN      
        1 cctgaagtct atattttcgg caacatgatt tccatagagt atccttatgg agtttttatt
       61 ggcaccaaga gacatcgttt ttgtagcgct caataacatg ttagctttag tttgatcgac
      121 tttagccata ttcaataatc cttcaagagc actttatttg ccaaagtatt gaacacatcc
      181 tgacggtggc atgtaaacat agtgtcgtaa cgcagtaatt ccattaaatc gtcactaatt
      241 ttacccaaac gaatatcggc agatatttcg tatgcccaag caaatacctt tgattttagc
      301 gtagcactgt catcccccag acctggcgta aagggaattg ttcgtggaaa gagaacgaca
      361 tcattaatta atttaagtga tgacatcccg acatctttta ttgcttcaac ggtggcaggt
      421 tttaatccaa aaaagatact aacaaactca ggtgcaagca ttgacataaa gaaaacttgc
      481 attaagtaat cattttgaat gtctacacac ttagaacgta gttgctgaat ttttctcgga
      541 ttataaaatt cctcctgccc gctgagcaaa gttacttttt caccttcatt gtaaatgccg
      601 aagtcagtat cttcactgtc atattctcga atgacgttag tgtcgccatc aactacagct
      661 acaaagaatt tgtcccaaga gatctcttta cttaaagaac gaggaacgag gaagcttcct
      721 ggccgagttt caacttcaaa tgcattatca atcatagctc caaatccatt accaaatagt
      781 cggcgttgga ggagtggtga tccagtttga tacttagaag atccatgaag aggcaataac
      841 attaagaagg cacggtaact acgaataatc gaaatctcgg tacgtaatag tttaccgagt
      901 aaattacgcg tgatgttatc tgattctgac atgtgcggat tgtccttgcg ccaatacaat
      961 tgtacgaatc ttctacatga aattaaggtc tggattctga aggatgaaaa gtaacttttg
     1021 gaattttaat actgccgctt tgaaccttaa aactaacctt tcagccatta cccttacttg
     1081 taacactttt taaccaaact aaccagaatt ttatcaaaaa accctgacct tgctaacgaa
     1141 atgtgaaccg tataaaaaaa cctcaaagcc tgagtcgtta tcggcattaa gtagcaataa
     1201 ccttagggaa aaatgcgcgt aagcatgatt attttttcga ttgtttaaca tgactactac
     1261 atagggtcac tttttgctga aaagtggccc tatgcaccat cgacataggg tcacttcttg
     1321 atgaaaagtg accctatgta atcacgacat agggtcactt tttttaaaag tggcactatg
     1381 cgtcaaaatc atagggtcac tttcctttaa aaagtggcac tagatagatg tataacttag
     1441 taggtaaaac agttcttttc agatcacgat ctcagcaata agcattgctg gaaaagttac
     1501 ttttcttcca cgataaaatg cggacctttt ggccgttccc gtttacatct gatgtcactc
     1561 actctcagtg cttcctccat tcttaaattt gccatgcaaa attctcgaaa aaattcacag
     1621 aaaaaagtcc cgttcgtgaa aaaaaattga aaatcacttc ggtatagtga tttcacatca
     1681 ggaggcccca tgtctaaatc gaagctatta aagtcattgg atatttcacg tactttattg
     1741 cgctcacaaa tcgatgttct tcgagaagag gctgaagaac tgatcaccaa ttattggatc
     1801 gagtggaaag aacggaatca cattgagagg aatttaccca gggatagcaa aactctaaag
     1861 aaggagtatc tgggttcata tgcaccaaaa gttgagctaa ttggtaatgc caggaaagtc
     1921 accattacct ggcatcagtt tagcccgtat aaaaacaggc cacctagtca catgtcgaaa
     1981 cggatttcac cgatgaaaag tggaaaatat acgaaaagct gctttgttaa tcatgccagc
     2041 tgggaatatg aaatgattga aaaaacggag ggattactgg aaccctacag agaattactt
     2101 gagttctacc atgctgcata tatcgaactg ggacgaaaag tccgtcaata ctcaaaatca
     2161 gaggtggcac aatgaccgat gtaacaatga atgaaagcga gcataaggta cctgacgtaa
     2221 atacccgctc ccctcgtgcg tccgataata gccgtagggt cgtgcaaagt aaaaatgaac
     2281 attacggcat ggctacagta actattaccc caggcacgcc agactttaac cgatttctca
     2341 cggcacgtaa tcgttccgta ataagagggt ttgatgatgt cagtattgcc atttcatcct
     2401 tattcagaac ggtcaatgca gttaagcacc ccgaactagt gcaggcaatt caagactggt
     2461 tcaatgaatt gcacgaagaa aataatctaa tgaagaacaa tctggagcgc cacattgcca
     2521 ccatccatat cgatgaaagc gatccgttct tctcatcaac tgagttttca ccgttcagat
     2581 ttgaacctgt acagttaaac ttcaacaatc aaaacacgat gcgcttttat aagcacatct
     2641 ttgaaatgaa caaccttctg acacagatgc acaaattcaa ctctctgggt caattaccgg
     2701 ttagcgatta caacgttatg gcccacaata ttatccggag ccttaatatg tatatcgagc
     2761 gggtcaagaa aacacttaac gtttcccgcc gcgcaaaagg tacttacagt cctgatgagt
     2821 tcatcgaaaa ggtaaagcag tataaaagcg tgcaagcata tattgctgcg gaactgtctg
     2881 gtaaacgccg gtaaggatta tttgatgaat ttcagggcac tcttcttaag tatgcaacga
     2941 gtttttggca ttttctccag aagggagaat gatgtatctg agttaatgat gaaggatgca
     3001 gcgaacttct cgccatttgc tcagattata ggagagcaga aatacactgt gccggaccac
     3061 ccgaatcctg aagtcctgaa attcattgaa tacccaacca ggcctgcggg gatacagaca
     3121 tttaatgaac agtctattct ttcgttgtac agagacaagc tgcactctat ttctatgatg
     3181 ctggcaataa gtgatggaga cattcgggag gatgcctata ccttcactaa tctggtgtta
     3241 aaaccattga ttgaatacat ccgctggatt catttacttc ctgcgtcaga gaatcatcac
     3301 cataatggta ttggaggatt gttgtctcac agcctggagg tggcgatgat ctctttaaaa
     3361 aatgccaatc actctgaatt acgtccgatt ggctatcagg atgaagaagt ggttcgccga
     3421 aaagtttatc tgtatgccgc ttttatctgc ggcctagtac atgatgcagg gaaggtatat
     3481 gaccttgata tagttagtct caacttgagt gaaaccttaa catgggcgcc aagctcgcaa
     3541 agccttcttg actgggcacg agaaaacaat gttgttgaat atgaaataca ttggcgaaaa
     3601 cgtattcata accaacataa tatatggtcc agcgtatttc ttgagaggat tttagatcct
     3661 gtctgtatgt cattccttga ccgggttaaa aaggagcgtg tctatgcaaa aatggttacg
     3721 gcacttaatg tctataacga cggcaatgac tttttatcaa aatgtgtaag aacttctgat
     3781 tattactcta caggtactga tcttaatgtg ttgagagacc cgataatggg tcttcgttcg
     3841 aatgatgctg cggctagggc aataggtacc attaagcata actttactag cattaatatt
     3901 aataactata aatctaaacc catgcacatc attattgtaa acggtgaagt ttacctcaat
     3961 gaaaacgcct ttctcgactt tgtactgagt gattttgcag cccataagtt caacttcccg
     4021 cagggagatg caggcaaaac agtacttgtc gaatctctgg tgcaacgggg ttatgttgaa
     4081 ccctacgacg atgaacgtgt ggttcattac tttattcccg ggacatattc ggaaaatgat
     4141 attgcaagca tcttcaggaa cggtattggt aagctggaat tttataacct cttaaaactg
     4201 cgttggatcg ggttaatttt tgattcatat aaaatcccgg attcagttcc tggtctgttc
     4261 agcgttaatg cgaataaaga tttcatctat attgatgagc agaaaactgt aacggaatac
     4321 agaaggccgg tcccgggtcg tgagtctgtt accagagtaa cggacactgt caatgacgcc
     4381 attgagaata ccccacagta tggacttcag ctcgtaaacg gccctgatgc cgacagtaac
     4441 aatattatta gtagtgaaaa tactgaagcc attacggata gtttggaaga gtctggcgcg
     4501 gatatctcaa acgagatttt tgaaacgcaa gttgttacgg caattgatac tgccgaaacg
     4561 gtaaacgcgg atgagccaga acaggtagaa gagcatgacg accggagcca gattcatctg
     4621 gttgaacaac tgcatgaaat gctgttgagc gccccactgc cccatcatgc cgtcattaat
     4681 attgattccg taccttacct ggatctcgat gctgcgattg cgttaatccc gggtatagat
     4741 gaggcggaat tttgtaatgg gccatttttc cagttgacat accgtgatgg gtctctagat
     4801 ggtatgtgga tagtccgtga tgtaaacaat ctacgcctga tacagctggg ggataactgc
     4861 gccggtatgc aagtaagcac atcggaacca cgaaacacgt catcgctgaa atcactattt
     4921 gatacgtcaa tgtaccaacc gttggatatc cctgaagctc catcagtaaa cgaagctgct
     4981 tcgcctccac aaacaccgct tgaactcccc cgaccacgac tgaatgcacc tgtggctgaa
     5041 gaggccagct cggtagctga acagactaac gcccactccg aaccggagtc cgtcatcgct
     5101 actgagtatg agcaatatgg tcatttgctg gaagagaccc ttgagagcga tggtgaggca
     5161 tattctgatt taattgcctc tgacagtact gaggcagaat atcccgccac tgatccgcag
     5221 agcagcgatt ttgcccaatt acctggtgaa acggccctgt ccgttgcgcc cggtgatttg
     5281 gattactcag agggtgccat taagccacca gcacccgatg caaccggaaa agaaacaata
     5341 ctaacaagtc ctgaaccagc agaggatgtc cgagaaacgg tagccgccgt ggagaaagcg
     5401 agtcatcttt ctccggcatt ggctcgactc tttgctgtaa gcacccatgc tgagaagaag
     5461 catgaaaaaa cgcaggaacc ctcccctgtt aaagaggtta aaaatccaac ctcctctacc
     5521 acagttaagg ctcctattcc cattgaacca ccaggcgctg aagagaaaga ggcggtggaa
     5581 gaattcaccc tgttaaacga cggtgaagtc acagaactcg aatatgtcga gattgccacc
     5641 atgctccacc aaattctgac gaagttatct gggtcgttca aaagaaagag gaaaaaccgg
     5701 ttcatggttt tgacccagaa caccttttat ctgacgcagt catgtattga aaagtacgga
     5761 acgcagctaa atgcacctga gctttttaac caacttcctc agtaccaggt cacatccggt
     5821 gccgtagtca atacaaaatg cattgcattc aacataccaa ctctggtcgc cgcatcagac
     5881 cgggcaaagg tcgatattga gttaattatt aacaagctaa aagaggtcgg gaatctgtaa
     5941 tgacaaaatc aaaaagaacc aacctgcacg ctcaggaaaa cttttacagg ccgatactgg
     6001 agtacagaag tgcctcagtg cttttaattt gcgccgtcat aatgctggtt atgggctttc
     6061 gttcagatgg ggtgaatatt gcccctatca ttctttatac cgctgttttc ctgcttttgg
     6121 tttgtttgta ccgttgtaaa acggcacacc cctatttgat ggcacactgg cgcgtatttc
     6181 agcgccagat aatgtttatt agcctcaagt cgctcaggac gatcaataaa agtaactttt
     6241 tctcgaacga gcgaaagtat cgccagctgg tacaagagta taaaaaaact aaccgaccag
     6301 taccggatag aaaaacctac ttttgtaacg gctttgaatg gggtcccgag catgcagacc
     6361 gcgcatatca aattgctaat ctctcaagtg acaaaagaga gatagccctg ccatttgttc
     6421 taagccctat agcgcgacat tttgaaacaa tggccagatc catggggggc aataacgcca
     6481 tctttgctgt tgaccggcgg gcacccattt ttgtgaccga agataactgg ttcggacata
     6541 ccctcattac cggaaacgtc ggtaccggta aaacggtact tcagcgctta ctgagcatca
     6601 gcatgctgca tttgggccat gtcattgtgg ttattgaccc gaagaatgac gctgagtggc
     6661 gacagtcact gatggatgaa gcgtcagaac tggggttgcc attttataaa tttcacccag
     6721 cccagccgtc ttcatcggtg tgtattgatg tatgcaatgc ttacacaaat gtttcggacc
     6781 tcacctcacg acttcttagt ctggtttcgg ttcccgggga agttaaccca ttcgtgcaat
     6841 atgccgaagc tctcgtctca acggtgatca ctggtctgtc ttacacggat aaaaaaccgt
     6901 cgatatatct catccacaaa aacatgaaaa gccatatgtc tgttgtgaac ctaacgataa
     6961 aagtaatgga atgctgcttt gcacgacact acgggcctga tgtctggatg gaaaaggtga
     7021 aatacgcttc gaatgacact cttcaggttc gatttaagcg cctgacagaa tggtttaatg
     7081 cccattttct taattatgaa ggcgccgagc ccattgaatg gattgatacc gtcggtcggt
     7141 tagtggatta ctccatgtct gatcctgaac acatgtctaa aatgacggcg ggtattatgc
     7201 cgctatttag tcgactgact gaacaaccac tgaatgaatt gctttcgccg tctcctaata
     7261 cactcacttc gcgtgaaata gtcacttcag atgggatgtt cagtaccggc ggagttctgt
     7321 atatctcgct cgatggcctt tccaacccgg aatcagcccg ggccatttcg cagttgataa
     7381 tgtcagatct tacgtcttgt gcgggtagtc gttataacgc tgacgatggt gatatgtctt
     7441 cccattcaag aatcagtatt ttcgtcgacg aagcacactc tgccataaac aattcaatga
     7501 taaacttact cgcacagggt cgtgccgctc aaattgcatt atttatctgc acgcaaacta
     7561 tttccgactt tattgcagca gcgaacgctg agacggcgaa ccgtattaca ggtctatgta
     7621 acaactacat tagcctgcgg gtaaacgaca caccaacaca aacgctggtc gtagaaaact
     7681 ttggtaagag tcccattagt acaaatatgg tcacgtatac gacaggatct gaaaccactt
     7741 tgccgcataa taacttctct gggtcaatat cagaaagaaa gcaaacaacg ctggaagaaa
     7801 gtattccaaa agagcttttg ggccaggtcc caaaattcca tattgtggcc agacttcagg
     7861 atggtcgtaa agttgtcggg cagatcccga ttgccgtaag tgaaaaagcg atgaaaccca
     7921 atacgacgtt actggagatg ttcctgaaat ccgccggaaa agtaactttg cgccagaatg
     7981 tgggtttaag ctatttaaat aaatatctaa ggaaattgca ttgatggatg agcaaattac
     8041 tctgacacaa aatcaaattt tcagtgcatc tctgaaagta agtaaaagcc gctcgctcgt
     8101 taagcggcgt atgcaatcat tagggttgaa gtttaccgaa tcgcaggatg tccgtaaccg
     8161 actggcgggg atagaaaaag gtgctttgaa atgtgtgggg caattttgcc atgacaacga
     8221 tgctgagtct ttatcagcga tggcaatcat cctttccgag ctttttctgc tgcaaggcga
     8281 actgtccacc agcaatgaat acggtgacca tgaaacgagt tactggaccg tagcacaagg
     8341 cccatgtgat gaatgggtcc agtctctgct ggcgagcgaa aatggtcgac gtacatttaa
     8401 tagctttcgt ataactttcg acaactccga agagcgccgc agcctcgtcg aaaaaaatgc
     8461 caaaatgctg gggagttatt tattgcctta ttttgtcaat tttacaaatg ctgcatctgc
     8521 gtttattacg ctgcctaata gcatcacgtt taaacaggtg caacgcaaca agccgctcat
     8581 tcaccctgaa accacattaa gtcacattct cacaatagaa gattcggcgt ttctctctcg
     8641 gatcaaattt aaacttatct cggcaattga ccgtctgccc gacccgtccg gtcagtatgc
     8701 caacatgttc aatcacatca tggatcgggc gctgctgacg cacctcaacc gggagcaaat
     8761 tgatagccct tgtgtgtgca aaaaggtcat ttccacctat gcagacacta tgctgacgct
     8821 cccgatattt aatacgacga ttaccgggaa ataccggcac tggaccccgt gggggattaa
     8881 ttttgtcgag tttagtcgtc aggctgcaaa ggaaaaatct tgtgtctatg ttcctgaacc
     8941 cggccaaatt cactggaaaa gtccggagca taaggaactg gcagagtaca gcctgatcaa
     9001 ccagataatc ccgcagcaat accactggtt gctgggtgtg ccgacaatct ggcgcagtca
     9061 ctatcgtgac catagcaaac gattagactt attcaaggag tggagggacg caaatggctg
     9121 cggataattc tgctcgtgca gtattcatca ggggactgct tttactaggg atgatgcttt
     9181 atcttattta tagtctgggg ttccagaaca ccgacgagct aaagcaacaa atcactcagg
     9241 aggtgaacgc cagtcgtagc cttatttcta atgaccggtg gttatctgtt attgcaaata
     9301 gtgaggctac tttatccacc ctgattaatg actataagct gatcgactat ctgaatacga
     9361 tactgatccc ggacacatcg acacctgcac gtggaatgaa ttacgttgcc gaaaagatgt
     9421 catcagtaaa ctacactatg gcaaaaaatc tacccctgct gatttatcag tccattttcc
     9481 gatggaatct gatactcggt tggctgattg tgttttcacc atacctaatt gctatgctcg
     9541 ctgatggcat gtatcagtgg aaattaaaac gttacgtttt cggtaatgta accgttcagt
     9601 tttacagaat ctggttcagg gccttctgga taattggtgc attaaccttt atatacctat
     9661 caatgcctaa tatgtcactt tttaacaata tcgctcaact gttcccccct gtcgcattgt
     9721 tgatattggg aattgcactc aaccgtttgt ggtctaactt tcaaaaactt atgtaataag
     9781 gtggtcagat gtttaaacgt aataataaga agtcacctgc caatatcaaa gaatcgcttg
     9841 atgagaacgc tgaccgtttc tataaaatgt tccgaatcca taccacgtca aaagtggtta
     9901 tttcactgct ggcattagcc gccgcagggg tttcggcctt taatatttac gataagtatc
     9961 aggaatccga aggacgaatg gatcatatcg ccgtcgtgcg tatctcggga gaaatgggta
    10021 ccggttctga ggtcggcgat ggatctgtga tagccacagc actggcgaaa gcgtacaaca
    10081 acccacatgc caaagccgtc attattgaag ctgagtcagg tggcggcggt ccttcagacg
    10141 cgattattat ttaccgccag attaatgcac tacgaagcca ccagcagaag attgaacggg
    10201 tttcacatgc cgaatccaca agcaagcccc gtaacacgag tgatcacagt gcttcacgtt
    10261 cgcaggccga tagcaatgcc gaactgtcga aacgtaatac gctggacgtt ctctccgccg
    10321 ggagtgggca tttcatttct gatatggaaa acagctacaa gcctattatc gtcagcgtca
    10381 aaggaatttg tgcgtcagca tgctactacg ctgtgtcccc tgctgacgcc atttatgcag
    10441 acagtagcgc tctgaccggt tcgatcggtg tccgaatgga tcactggaac ctgtctcgcg
    10501 ttatggatac tgtcggcgtt aaaaatgagc cactcaccgc cggggagttt aaagatgcac
    10561 tcgaccccta tcatccactt tctgatgcca cccgcgattt tatgcagaag caaatcctgg
    10621 atgcaatgca cgaacaattc attgcggacg ttgagcaagg caggggtaag aaattattaa
    10681 gccgtccgga ggctgatgct gtcgcactgt attccggcag ggtatggaca acgcagcagg
    10741 cgatccggta cggactcatc gacggtgacc tgacacctgt cgaagtccgt tcacgcctgt
    10801 cccaaatgta ctcaaccact atcttcaaga cgtacaacga gccacaacgg tcgcttcgct
    10861 ccgcgctcgg gatgttaatg agtctgtcga ctaacgtcga aacgctggca ggaacaacct
    10921 ctcggatact tgattctgtt caggccacca gctatccgac ggtgagataa gatggatgca
    10981 attttgatgg tgtggcgagt gataggaatc agcattattg tcctagtact cattgtgctt
    11041 tactgcctga tgggatttgt atcgtcggcc attgctgaac gcaggacagc cagacagatt
    11101 gaaatatacc tgcctgatga tgcagaaggg cttctgagcg acttaacgct gccggtcaat
    11161 gccaccggca ccacgcagat tgatcacgtt cttatcgcct ctcatggact ctacgtcatc
    11221 gagcagaaga actatgcagg aaagctctat ggga
//
LOCUS       DAFNBJ010000151        11183 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000151 DAFNBJ010000000
VERSION     DAFNBJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.151"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            18..317
                     /locus_tag="L2X49_003892"
     CDS             18..317
                     /locus_tag="L2X49_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBN4798905.1"
                     /translation="MPAVIDKALDFICAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(437..616)
                     /locus_tag="L2X49_003893"
     CDS             complement(437..616)
                     /locus_tag="L2X49_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBN4798906.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            690..2051
                     /gene="pabB"
                     /locus_tag="L2X49_003894"
     CDS             690..2051
                     /gene="pabB"
                     /locus_tag="L2X49_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBN4798907.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2055..2633
                     /locus_tag="L2X49_003895"
     CDS             2055..2633
                     /locus_tag="L2X49_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBN4798908.1"
                     /translation="MEYRSLTLDYFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2817..4181
                     /gene="sdaA"
                     /locus_tag="L2X49_003896"
     CDS             2817..4181
                     /gene="sdaA"
                     /locus_tag="L2X49_003896"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBN4798909.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETSYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4312..5910
                     /locus_tag="L2X49_003897"
     CDS             4312..5910
                     /locus_tag="L2X49_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN4798910.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGGTALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5914..7470)
                     /gene="yoaE"
                     /locus_tag="L2X49_003898"
     CDS             complement(5914..7470)
                     /gene="yoaE"
                     /locus_tag="L2X49_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBN4798911.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            7933..8904
                     /gene="manX"
                     /locus_tag="L2X49_003899"
     CDS             7933..8904
                     /gene="manX"
                     /locus_tag="L2X49_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBN4798912.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8967..9767
                     /gene="manY"
                     /locus_tag="L2X49_003900"
     CDS             8967..9767
                     /gene="manY"
                     /locus_tag="L2X49_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBN4798913.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9780..10631
                     /gene="manZ"
                     /locus_tag="L2X49_003901"
     CDS             9780..10631
                     /gene="manZ"
                     /locus_tag="L2X49_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBN4798914.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10686..11144
                     /locus_tag="L2X49_003902"
     CDS             10686..11144
                     /locus_tag="L2X49_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBN4798915.1"
                     /translation="MTIRDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2732 a         2823 c         2831 g         2797 t
ORIGIN      
        1 attttaagga taagaatatg cctgctgtaa tagataaagc cctggatttc atttgtgcca
       61 tggatgtatc agcgccaaca ccaagttcga tgaatgaaag cacggcgaag ggaatcttta
      121 aatatttaaa agaactggga gtacccgcga gtgccgctga tattacggcg cgagctgacc
      181 aggaaggctg gaaccctggg ttcacggaaa aaatggttgg atgggcaaaa aaaatggaga
      241 caggtgaacg ctctgtgatt aaaaatcctg aatacttttc aacatatatg caggaagaac
      301 taaaagcact ggtctgagtt aaatttatat cagcataaat gggtcaggac gcttttaatc
      361 acatataaaa agcgtccttt ttctcacaat caaatgatca tcggcttcta taacgaaatc
      421 gatacggatt agtgtgttac tcgtcttcat cctcaaaacg cgccacgatt cgctcgccgg
      481 agtggttggc acgcagctct tctgccacca gcgcgattgc ctggccgctg ctcatccctt
      541 gtgccatcag ttcctggatc cgctcgacag ctttttgctg ctgttcatgg gtgagtgaag
      601 gtaaacctgc aaacattgtt aactcctgct aaattgttgg cgctaattat ttcatgctac
      661 ccggcacata gccagtagag tcaggactga tgaagacgtt atctcccgct gtgattactt
      721 taccctggcg tcaggacgcc gctgaatttt atttctcccg cttaagccac ctgccgtggg
      781 cgatgctttt acactccggc tatgccgatc atccgtatag ccgctttgat attgtggtcg
      841 ccgatccgat ttgcacttta accactttcg gtaaagaaac cgttgttagt gaaagcgaaa
      901 aacgcacaac gaccactgat gacccgctac aggtgctcca gcaggtgctg gatcgcgcag
      961 acattcgccc aacgcataac gaagatttgc catttcaggg cggcgcactg gggttgtttg
     1021 gctacgatct gggccgccgt tttgagtcac tgccagaaat tgcggaacaa gatatcgttc
     1081 tgccggatat ggcagtgggt atctacgatt gggcgctcat tgtcgaccac cagcgtcata
     1141 cagtttcttt gctgagtcat aatgatgtca atgcccgtcg ggcctggctg gaaagccagc
     1201 aattctcgcc gcaggaagat ttcacgctca cttccgactg gcaatccaat atgacccgcg
     1261 agcagtacgg cgaaaaattt cgccaggtac aggaatatct gcacagcggt gattgctatc
     1321 aggtgaatct cgcccagcgt tttcatgcga cctattctgg cgatgaatgg caggcattcc
     1381 ttcagcttaa tcaggccaac cgcgcgccat ttagcgcttt tttacgtctt gaacagggtg
     1441 caattttaag cctttcgcca gagcggttta ttctttgtga taatagtgaa atccagaccc
     1501 gcccgattaa aggcacgcta ccacgcctgc ccgatcctca ggaagatagc aaacaagcag
     1561 aaaaactggc gaactcagcg aaagatcgtg ccgaaaatct gatgattgtc gatttaatgc
     1621 gtaatgatat cggtcgtgtt gccgtagccg gttcggtaaa agtaccagag ctcttcgtgg
     1681 tggaaccctt ccctgccgtg catcatctgg tcagcactat aacggcgcga ctaccagaac
     1741 agttacacgc cagcgatctg ctgcgcgcag cttttcctgg tggctcaata accggggctc
     1801 cgaaagtacg ggctatggaa attatcgacg aactggaacc gcagcgacgt aatgcctggt
     1861 gcggcagcat tggctatttg agcttttgcg gcaacatgga taccagcatt actatccgca
     1921 cgctgactgc cattaacgga caaatatact gctctgcggg cggtggaatt gtcgccgata
     1981 gccaggaaga agcggaatat caggaaactt ttgataaagt taataagata ttacgccaac
     2041 tggagaagta agacgtggaa taccgtagcc tgacgcttga ttatttttta tctcgctttc
     2101 aacttttgcg cccgcaaatt aaccgggaaa ccctaaatca tcgtcaggct gccgtgttaa
     2161 tccccatcgt ccgtcgaccg caaccggggt tgttgctgac tcagcgttca attcatctgc
     2221 gtaaacacgc tggacaagtg gcattccctg gaggtgcagt cgatgacacg gacgcatcag
     2281 ttatcgccgc cgcgctgcgc gaagctgaag aagaggtcgc tataccgcct tccgccgttg
     2341 aagttatcgg cgtgctgccg cccgtcgata gcgtaactgg ctatcaggta accccagtgg
     2401 tcggcattat cccgcccgat ctgccgtatc gcgccagtga agatgaagtc tcggcggtgt
     2461 ttgaaatgcc gctcgcccag gcattacatc tgggtcgtta tcacccttta gatatctacc
     2521 gccgtggcga ttcacatcgg gtatggctgt cctggtacga acagtatttt gtatggggaa
     2581 tgaccgcagg cataattcgt gagctggcgc tgcaaattgg tgtgaaaccc tgactatact
     2641 tatctttaca tctacaaaac actacttgag acaatcatcg caatattagt taaatcgcgg
     2701 tttttgatta gtttaattca tgtgaatagt taagccagtc gccgcgttcc ctcttacact
     2761 atgcgctgtt attagttcgt tactggaagt ccagtcacct tgtcaggagt attatcgtga
     2821 ttagtctatt cgacatgttt aaggtgggga ttggtccctc atcttcccat accgtagggc
     2881 ctatgaaggc gggtaaacag ttcgtcgatg atctggtcga aaaaggctta ctggatagcg
     2941 ttactcgcgt tgccgtggac gtttatggtt cactgtcgct gacgggtaaa ggccaccaca
     3001 ccgatatcgc cattattatg ggtcttgcag gtaacgaacc tgccaccgtg gatatcgaca
     3061 gtattcccgg ttttattcgc gacgtagaag agcgcgaacg tctgctgctg gcacagggac
     3121 ggcatgaagt ggatttcccg cgcgacaacg ggatgcgttt tcataacggc aacctgccgc
     3181 tgcatgaaaa cggtatgcaa atccacgcct ataacggcga tgaagtcgtc tacagcaaaa
     3241 cttattattc catcggcggc ggttttatcg tcgatgaaga acactttggt caggatgctg
     3301 ccaacgaagt gagcgtgccg tatccgttca aatctgccac cgaactgctc gcgtactgta
     3361 atgaaaccag ctattcgctg tctggtctcg ctatgcagaa cgaactggcg ctgcacagca
     3421 agaaagagat cgacgagtat ttcgcgcatg tctggcaaac catgcaggca tgtatcgatc
     3481 gcgggatgaa caccgaaggt gtactgccag gcccgctgcg cgtgccacgt cgtgcgtctg
     3541 ccctgcgccg gatgttagtt tccagcgata aactgtctaa cgatccgatg aatgtcattg
     3601 actgggtaaa catgtttgcg ctggcagtta acgaagaaaa cgccgccggt ggtcgtgtgg
     3661 taactgcgcc aaccaacggt gcctgcggta tcgttccggc agtgctggct tactatgacc
     3721 actttattga atcggtcagc ccggacatct atacccgtta ctttatggca gcgggcgcga
     3781 ttggtgcatt gtataaaatg aacgcctcta tttccggtgc ggaagttggt tgccagggcg
     3841 aagtgggtgt tgcctgttca atggctgctg cgggtcttgc agaactgctg ggcggtagcc
     3901 cggaacaggt ttgcgtggcg gcggaaattg gcatggaaca caaccttggt ttaacctgcg
     3961 acccggttgc agggcaggtt caggtgccgt gcattgagcg taatgccatt gcctctgtga
     4021 aggcgattaa cgccgcacgg atggctctgc gccgcaccag tgcaccgcgc gtctcgctgg
     4081 ataaggtcat cgaaacgatg tacgaaaccg gtaaggacat gaacgccaaa taccgcgaaa
     4141 cctcacgcgg tggtctggca atcaaagtcc agtgtgacta atacttctta ctcgcccatc
     4201 tgcaacggat gggcgaattt atacccgctt tctcgtctgc tgtaatattc cccactacac
     4261 ttccactgtt gcgtcaggcg tttgtcgcca tacgcttaca gggtggcccg catgcaaaaa
     4321 gcacaacgga tcattaaaac ctatcgccgt aatcgaatga ttgtttgtac gatttgcgca
     4381 ctcgttacgc tcgcttcgac cctgagcgtg cgatttattt cacagcgtaa cttaaatcaa
     4441 caacgggtag tacaattcgc caatcacgct gtagaggaat tagataaagt actgcttccc
     4501 ctacaggcag gtagcgaagt cttgcttccg ctgattggtc tgccctgctc tgtcgcccat
     4561 ttgccattac gtaaacaggc ggcaaaactc caaactgtgc gatccattgg cctggtgcaa
     4621 gacggcacac tttattgctc cagcattttt ggttatcgca atgtgcccgt cgtggacatt
     4681 ctggctgaac ttcctgcacc gcaaccactt ttacgcctga cgatcgaccg tgccctgatt
     4741 aaaggcagtc cggttttgat tcaatggacg cctgcagcgg gcagtagcaa tgctggggtc
     4801 atggagatga ttaacatcga cttactgacg gcaatgctcc ttgagccaca actgccgcaa
     4861 atcagtagcg ccagcctgac ggtggacaaa cggcatttgc tctatggtaa tgggctggta
     4921 gattcccttc cgcaacctga agacaatgaa aactatcagg tttcttcgca acgctttcct
     4981 tttaccatta acgttaatgg tccggggggt acggcgctgg catggcacta tcttccaaca
     5041 caattaccgc tggcggtgct gctaagttta ctggtgggct acatcgcctg gctggcgacc
     5101 gcttaccgga tgagcttttc ccgcgaaatc aatctgggcc tggcgcaaca tgagttcgaa
     5161 ttgttctgtc agcctttgct taatgcgcgc agccagcaat gtattggtgt agagattttg
     5221 ctgcgctgga acaatccgcg tcagggctgg atttcaccgg atgtgtttat tcctatcgcg
     5281 gaagaacatc atttaattgt gccactgacc cgctatgtga tggcagaaac cattcgtcag
     5341 cgccatgttt tcccgatgag tagtcagttt catgttggca ttaacgtcgc acccagccat
     5401 tttcgccgtg gtgtgctgat aaaagatctc aatcagtact ggtttagcgc tcacccgatt
     5461 cagcaactga tcctcgaaat caccgaacgc gatgccttac tggatgttga ttatcggatt
     5521 gcccgcgagc tacatcgtaa aaacgtcaaa ctggcgattg atgacttcgg caccggcaac
     5581 agttcatttt cctggcttga aacattacgt cctgacgtgc tgaaaattga taagtcattt
     5641 accgcagcta taggttctga cgcggttaac tcgacggtga ccgatatcat catcgcgctg
     5701 gggcaaagac tgaatattga actggtggcg gagggcgtgg aaacgcaaga acaggcgaag
     5761 tatttgcgcc gtcatggcgt gcatattttg caagggtatt tgtacgcaca gccgatgccg
     5821 ctacgtgatt ttcccaaatg gctggcgggc agccaaccgc cgcccgcccg gcataatgga
     5881 catatcacgc ccattatgcc gttacgttaa tgcttactca tcttcatcgt gcgccggttg
     5941 ctctttaaca atgcgaacca gatcaacacg ataatcattg gcttcaatga tggtgatatg
     6001 cagtggccct acatcaatca catcgcccac acggggaatg tgaccatttg ccgagatcac
     6061 gaggcccgcg accgtcgcga tatcatcgtc atcggcaagg tgctcaacat caagcgcctg
     6121 ctgcaaggca tgcaaatctg taccgccttt taccagccag ccgtcaccat cagtaatgat
     6181 ttccggcgtt tcgtcagcgt ccgggaattc acccgcaatg gcttccagca catccagcgg
     6241 cgtgaccaga ccttgtacca caccaaactc gttggtcacg ataacaaagc tcccgcgagc
     6301 acgacgcagc acgcccaaca ggttgatcgg atcgagggtt tccgggacga taatcgccgg
     6361 agacgccgaa gcaatcgccg ccacatcaac gccctcttcc aacgccacca gcagttcttt
     6421 agcacgtaca ataccgatga tttcatccag ttcaccgcga cataccggga acagactgtg
     6481 cggtgaagag agcagttgct cgcggatttc atcgaccccg agattagcgt caacccagct
     6541 tatttcaccg cgcggcgtca tgatcccgcg cagagaacgc gacgccagcg tcagtacgcc
     6601 gttaatcatg taacgttctt cttcggcaaa tgcaccttcc gggatcggca tcggcatcgg
     6661 gttatcggca tcgtgctgaa cattggcctg acgtttcccg cccatcaaac gcaggatggc
     6721 atcggcagta cgcgctcgca gcggcaaagt cgactggtgg cgaataaagt tgcgacgcgc
     6781 aatctggtta aacacttcga tgatgatcga gaagccaatc gcggcataca ggtaaccttt
     6841 cggaatgtgg aaaccgaaac cttctgccac cagactcaga ccaatcatta acaggaagct
     6901 cagacagagc accaccaccg tggggtgctg gttaacgaat cgcgtcagcg gtttggatgc
     6961 cagcaacata accgccatcg caatcactac cgccgccatc atcaccggca gatggttaac
     7021 catccctact gcagtaatta ccgcatccaa cgagaagacg gcgtcaagga tgacaatctg
     7081 tgtgacgacc acccagaaac tggcgtagcc tttaccgtgg ccggaatcat gatcgcggtt
     7141 ttccagccgt tcatgcagtt cggttgttgc tttgaacagc aagaatatcc ccccgaacaa
     7201 cataatcagg tcgcgtccgg agaaggagaa atccatgacg gtaaatagcg gtttggtcag
     7261 cgtgaccatc catgaaatca gcgacagcag ccccagacgc ataatcagcg ccagtgataa
     7321 ccccagcaaa cgcgctttat cgcgttgttt tggcggcagt ttgtcagcaa gaatggcgat
     7381 gaagaccagg ttatcgatac ccagcacaat ttcgagaaca acaagcgtga gtagccccgc
     7441 ccaaattgag gggtccatta agaattccat gacaagctcc tgcttaagga atagctattc
     7501 gacgccagaa ataatgcagg cgtaacgaca aaatgcaaac gaaaggtgcg gcatagagag
     7561 ccagaaaggt aggcgttaaa aggcctgatg ctgaaatgac gtcggtgacg atccatactg
     7621 cgggctactg ccctatactc catggttgtt aaacgggagt taaacatatc agagacgcct
     7681 ctgatttggc aaagatttac cttcctttgc aaacgaatgt gacaaggata ttttaccttt
     7741 cgaaatttct gctaatcgaa agttaaatta cggatcttca tcacataaaa taattttttc
     7801 gatatctaaa ataaatcgcg aaacgcaggg gtttttggtt gtagccctta tctgaatcga
     7861 ttcgattgtg gacgacgatt caaaaataca tctggcacgt tgaggtgtta acgataataa
     7921 aggaggtagc aagtgaccat tgctattgtt ataggcacac atggttgggc tgcagagcag
     7981 ttgcttaaaa cggcagaaat gctgttaggc gagcaggaaa acgtcggctg gatcgatttc
     8041 gttccaggtg aaaatgccga aacgctgatt gaaaagtaca acgctcagtt ggcaaaactc
     8101 gacaccacta aaggcgtgct gtttctcgtt gatacatggg gaggcagccc gttcaatgct
     8161 gccagccgca ttgtcgtcga caaagagcat tatgaagtca ttgcaggcgt taacattcca
     8221 atgctcgtgg aaacgttaat ggctcgtgat gatgacccaa gctttgatga actggttgcg
     8281 ctggcagtag aaacaggccg tgaaggcgtg aaagcactga aagccaaacc ggttgaaaaa
     8341 gccgcgccag cacccgctgc cgcagcacca aaagcggctc caactccggc aaaaccaatg
     8401 gggccaaacg actacatggt tattggcctt gcgcgtatcg acgaccgtct gattcacggt
     8461 caggtcgcca cccgctggac caaagaaacc aatgtctccc gtattattgt tgttagtgat
     8521 gaagtggctg cggataccgt tcgtaagaca ctgctcaccc aggttgcacc tccgggcgta
     8581 acagcacacg tagttgatgt tgccaaaatg attcgcgtct acaacaaccc gaaatatgct
     8641 ggcgaacgtg taatgctgtt atttaccaac ccaacagatg tagagcgtct cgttgaaggc
     8701 ggcgtgaaaa tcacctctgt taacgtcggt ggtatggcat tccgtcaggg taaaacccag
     8761 gtgaataacg cggtttcggt tgatgaaaaa gatatcgagg cgttcaagaa actgaatgcg
     8821 cgcggtattg agctggaagt ccgtaaggtt tccaccgatc cgaaactgaa aatgatggat
     8881 ctgatcagca aaatcgataa gtaacgtatt gtgttgatta tcactcagtt ttcacactta
     8941 agtcttacgt aaacaggaga agtacaatgg agattaccac tcttcaaatt gtgctggtat
     9001 ttatcgtagc ctgtatcgca ggtatgggat caatcctcga tgaatttcag tttcaccgtc
     9061 cgctaatcgc gtgtaccctg gtgggtatcg ttcttggcga tatgaaaacc ggtattatta
     9121 tcggtggtac gctggaaatg atcgcgctgg gctggatgaa catcggtgct gcagttgcgc
     9181 ctgacgccgc tctggcttcc atcatttcta ctattctggt tatcgcaggt catcagagca
     9241 ttggtgcagg tatcgcactg gcaatccctc tggccgctgc gggccaggta ctgaccatca
     9301 tcgttcgtac tattaccgtt gctttccagc acgctgcgga taaggctgct gataacggca
     9361 acctgacagc gatttcctgg atccacgttt cttctctgtt cctgcaagca atgcgtgtgg
     9421 ctattccggc cgtcatcgtt gcgctgtctg ttggtaccag cgaagtacag aacatgctga
     9481 atgcgattcc ggaagtggtg accaatggtc tgaatatcgc cggtggcatg atcgtggtgg
     9541 ttggttatgc gatggttatc aacatgatgc gtgctggcta cctgatgccg ttcttctacc
     9601 tcggcttcgt aaccgcagca ttcaccaact ttaacctggt tgctctgggt gtgattggta
     9661 ctgttatggc agtgctctac atccaactta gcccgaaata caaccgcgta gccggtgcgc
     9721 ctgctcaggc agctggtaac aacgatctcg ataacgaact ggactaacag gtgagcgaaa
     9781 tggttgatac aactcaaact accaccgaga aaaaactcac tcaaagtgat attcgtggcg
     9841 tcttcctgcg ttctaacctc ttccagggtt catggaactt cgaacgtatg caggcactgg
     9901 gtttctgctt ctctatggta ccggcaattc gtcgcctcta ccctgagaac aacgaagctc
     9961 gtaaacaagc tattcgccgt cacctggagt tctttaacac ccagccgttc gtggctgcgc
    10021 cgattctcgg cgtaaccctg gcgctggaag aacagcgtgc taatggcgca gagatcgacg
    10081 acggtgctat caacggtatc aaagtcggtt tgatggggcc gctggctggt gtaggcgacc
    10141 cgatcttctg gggaaccgta cgtccggtat ttgcagcact gggtgccggt atcgcgatga
    10201 gcggcagcct gttaggtccg ctgctgttct tcatcctgtt taacctggtg cgtctggcaa
    10261 cccgttacta cggcgtagcg tatggttact ccaaaggtat cgatatcgtt aaagatatgg
    10321 gtggtggctt cctgcaaaaa ctgacggaag gggcgtctat cctcggcctg tttgtcatgg
    10381 gggcattggt taacaagtgg acacatgtca acatcccgct ggttgtctct cgcattactg
    10441 accagacggg caaagaacac gttactactg tccagactat tctggaccag ttaatgccag
    10501 gcctggtacc actgctgctg acctttgctt gtatgtggct actgcgcaaa aaagttaacc
    10561 cgctgtggat catcgttggc ttcttcgtca tcggtatcgc tggttacgct tgcggcctgc
    10621 tgggactgta agactgttgt acactaccgg ggccttttgg ccccgttttt ttatctggag
    10681 gattaatgac aatcagggac ctggtactga ttcttttcat cgccgcactc ctggccttcg
    10741 cgatctacga tcagttcatc atgccccgcc gtaatggccc caccctgctg gcaattcctt
    10801 tgctccggcg tggtcgcatc gatagcgtta tcttcgtcgg attgattgtc attcttatct
    10861 ataacaacgt cacgaatcat ggtgcgttaa taacgacatg gttattaagc gcactggctc
    10921 tgatgggttt ttatatattc tggatccgcg ttccgaagat catctttaaa caaaaaggtt
    10981 ttttcttcgc caatgtctgg attgaatata gccgaatcaa agcgatgaac ttgtcggaag
    11041 atggcgtgtt ggtgatgcaa ttagaacagc gtcggctgtt aatccgcgtt cgaaatatcg
    11101 acgatctgga aaaaatttat aagcttctcg tttcaactca ataagttatg aatttagcca
    11161 aagctatgtt tagtgtattt tta
//
LOCUS       DAFNBJ010000152        11170 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000152 DAFNBJ010000000
VERSION     DAFNBJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.152"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            90..1157
                     /gene="pilL"
                     /locus_tag="L2X49_003903"
     CDS             90..1157
                     /gene="pilL"
                     /locus_tag="L2X49_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HBN4798916.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGGNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSATAPAQDTATTISPAAMVTTSTSPESSTTANSQN
                     KPRNPFRGNSVSPSTPAAIPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            1157..1594
                     /gene="pilM"
                     /locus_tag="L2X49_003904"
     CDS             1157..1594
                     /gene="pilM"
                     /locus_tag="L2X49_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HBN4798917.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQQMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            1608..3290
                     /locus_tag="L2X49_003905"
     CDS             1608..3290
                     /locus_tag="L2X49_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HBN4798918.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            3283..4578
                     /gene="pilO2"
                     /locus_tag="L2X49_003906"
     CDS             3283..4578
                     /gene="pilO2"
                     /locus_tag="L2X49_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HBN4798919.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWTPVVKKQRVPSHHKHK
                     SHVKPRRLTSLKVTVGSRNSRGEISRLTGTGILTRPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLAMNEEPPEKWQVVSSLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVGPTA
                     EEIAARARLQFKKPEPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPAPSEQLMRVFTWFQKKQLTPAINEIAIQEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            4565..5017
                     /gene="pilP"
                     /locus_tag="L2X49_003907"
     CDS             4565..5017
                     /gene="pilP"
                     /locus_tag="L2X49_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HBN4798920.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVNLSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLHAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            5028..6581
                     /gene="tadA"
                     /locus_tag="L2X49_003908"
     CDS             5028..6581
                     /gene="tadA"
                     /locus_tag="L2X49_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBN4798921.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDITPKVVSLSELQEAGQDDENKGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     VMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEEAVKLAWSRAISSAMRLDPDAIMEGEMRDLVSMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRVPELSED
                     DRAYLERHCNKDSLCSTDNIWFRNPHGCSDCNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMHGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDA"
     gene            6594..7679
                     /locus_tag="L2X49_003909"
     CDS             6594..7679
                     /locus_tag="L2X49_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBN4798922.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLGLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKKQLHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPVLSKI
                     SSPDSWSGALGFLYGLSLFVDNYGAICAVLFAVVTGVISWSLKNWKSRSLADNIMPWS
                     IYKDIQGAAFLLNMAALLKAKMTTLNSLNVLQEFASPWLSTRLDSIIYRVRQGDHLGL
                     ALRQCGYHFPSRESANFLSLLQGDGATELIGNYGQRWLVQTLERVKKRAAVVRLIMLI
                     FLVMSLLLLVMAVVDIQSIGDNSMGSI"
     gene            7696..8310
                     /locus_tag="L2X49_003910"
     CDS             7696..8310
                     /locus_tag="L2X49_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HBN4798923.1"
                     /translation="MLIQNINANVLKKNEKETSHDKGWGIMEQGAIALVVIIVLALVF
                     GGLYMLRSRTGVANESSNIQTIITSTQGLLKGSDGYTFTSGAKMTGALIQMGGVPKTM
                     TVRGTPSSGTATLYNSWGGAVTVAPASTSGFNNGFTVTYEKVPQDACIQIATQISRTG
                     LTNGITLNSTAHSDGKVTTEEASAQCTADNGSTGTNKLIFTING"
     gene            8320..8880
                     /locus_tag="L2X49_003911"
     CDS             8320..8880
                     /locus_tag="L2X49_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBN4798924.1"
                     /translation="MSGGKVPLRLISLILLCSLIYPARASYIAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLKPGAININKDRKTGKASSTDYGLMQINSTHIPKLIK
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKVWRIYRDMKGICLPGQGGRQCRQS"
     gene            8865..9521
                     /locus_tag="L2X49_003912"
     CDS             8865..9521
                     /locus_tag="L2X49_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBN4798925.1"
                     /translation="MQTIVGISLSPVYILPLMFIFSIIGRTLLFRVRYFLSDTEHLWY
                     KTHTAVLSGIWLYSIAVALIILSSSPLIYRIHAVLILSFMLQMAVTDALTGLLPGTFT
                     RRFLIAGILSQITTDIWWLRATEIVSAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWSVLLGAGGFVLWHSTWCIKGHKEGPLGPWLCLGHVLLLLDNLYQPLWVI
                     "
     gene            9521..10813
                     /gene="pilV"
                     /locus_tag="L2X49_003913"
     CDS             9521..10813
                     /gene="pilV"
                     /locus_tag="L2X49_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HBN4798926.1"
                     /translation="MKKYDRGWASLEIGAALLIVMIIVAWGAGIWQDYLKTKGWQAEA
                     RLVSNWASAARSYIGKNYTTLQASSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNTQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNIGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGAILSCQSGTWRTIGGKLKVTQLSTTGYLGQFDFCAIARV
                     GNAEDAHYCQVVESPAGSRKWYKYEHKTGCIASCVTLN"
     gene            complement(10810..11058)
                     /locus_tag="L2X49_003914"
     CDS             complement(10810..11058)
                     /locus_tag="L2X49_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798927.1"
                     /translation="MFIYASGGNGGSAGGACVNTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
BASE COUNT         3163 a         2681 c         2593 g         2733 t
ORIGIN      
        1 attcggacgg aatgacagtc gtcattcgtc ctctccagcc gcctcacatc aattaacaaa
       61 ctcgtatata cgaacacgga gtctccgtta tgaaaaaaaa ctgtctgaca cttatactgc
      121 cgtcattatt gtccggctgt gccggaattt ccggcggcaa taacgtggca ccatcagata
      181 cgcctgctct ggtttttgtt gacggacaaa tcagtgagag tgctggcgtc ctggcactta
      241 cgcaacaacg actttctcca cctaaaataa ctccgccccc gagagtcagc gtgccagaga
      301 cagtaaaaac agcacagcca gtaagcgaat cccgaccgtt accttcacca acaataagtc
      361 agaatttctc ccggttaatc attacaggca agcctggtag cgcccctgta acactatcgc
      421 cgacggcccg taaccggacc gtcagtcagt ggataaaaac attactaccg gaaggatgga
      481 aattcagaca agagaatgca gccacgccca aactgaatac caaactggtt tcatggtctg
      541 ccaatgatca gtggacccgt tctctgaacc ggttgctgga agaacaacac ctctggggcc
      601 atctggactg ggataataaa acgttgaccg tcacaacatc ggctactgca ccggcacagg
      661 acacggcaac cactatctcg ccagcagcca tggtaaccac atccacttcc ccggagtctt
      721 caacgacagc aaatagccag aacaaaccac gaaacccatt caggggaaac agcgtatctc
      781 cgtctacgcc cgctgcaatc ccgactccga ccggctccac agtcaagtct atcccactca
      841 tgaccgggac acctgtcaaa ccggtatctc agggtaaaga gtggcgtgcg ccggcaggca
      901 ctacattaag ggaaaacatt attaagtggg cagaagaaac aaaatgcgaa agcatggcat
      961 caacgcactg gatggtcata tggccacttt ctgtaaccga ttaccgtctt gatgcgcctt
     1021 tgatgttccg gggatcattt gaatcagcca tggagcaggt atttgagtta tatcggaccg
     1081 cgcaaaaacc tttatacgca caggcctccc gcatgcagtg catcattaca gtttctgata
     1141 caagggatgg tcgctaatgg gatggctggt catggccgtc ggcctgacca ttctgattat
     1201 cactggaagc taccagaatc agcagatgtc tgaaaccacg aacgcccagc aatatgcaag
     1261 tgcgtctgta tgggcaagtc agatactaat gattgctaac agaatcaatg atatcagata
     1321 cgtatcaggc cagcaggacg gagtaatttc ttcagataaa ctggctctcc ctgtcactcc
     1381 ggactcccga attaaacatc agttacaaca gggacgtctg tgggtatgga tgcctgaaca
     1441 accaggactt gttgaaactt tgcgttcaaa aagtcgtggt tctgcactaa tagggatctt
     1501 ccaaaacggg caactcacct ggctatcagg aactgcaacc ggtctgacac ccccggcagg
     1561 cattactgct ggttctgtcg tctacgttaa ctaatctgga tatattcatg aaaaaatcac
     1621 accagcgttc aatgaagctg gcggtgctcc cctgcatgat cgccgtcgct ctttccatct
     1681 ccggatgtac tttcagcgaa atcaataaaa tgcagaaaaa agcacaggag gactcagcac
     1741 atgcacggga aaaggtatca gccctttctg cccgcaaatc acaggctctt acctggctcg
     1801 ataatcaatg gataaaccct gttccggtcg ctcaggtatc aagagagaaa aaacaaacag
     1861 ctccggcctg ctacatcacg caggcaagaa aaggagagat cactctacag gaactggggc
     1921 aacgtattac tgccgtatgc ggcatccccg taatcatcac ccctgacgca gccaattcaa
     1981 ctcttgaagg aggcgctacc cgccagatga ctgggacact accagcacct gatgaaaatg
     2041 gtcgtctgcc gttaagcagc ctgggcagca caacaatgac tacctccact cagccattaa
     2101 cgctgaataa cctcatgtgg cagggagata tcaatggtct tctggatttg atggccagcc
     2161 gaagcggtct gtactggcgc atggataatg gtcggattgt attctatctg actgaaacca
     2221 gaacgtatcc acttcatatg ctgaacacca aaaccagcag cagttccagc gtcagctctg
     2281 gctcaacaag cacaatgggg gcaacaggag gccaggataa ctcagcatcc ggtgatgcta
     2341 cgtcctctca gagcacaacc gtcggtcagg aatacgacct gtatgaagac atccggaaaa
     2401 ctattgaagc gatgctgaca ccagaaaaag gccgttactg gttatctgca tcgagctcaa
     2461 cgctaacagt cactgatact cctgctgtcc aggaagccgt cgcacgatat gtggacgaac
     2521 aaaacagtat tatgaaccgc caggtagccc tgaacgtaca ggttctgagc gtcagcaata
     2581 ccagaaacga gcagttcggt ctggactgga accttgttta caaatcgcta cattccgccg
     2641 gagcaacgtt gaacaatgca agcggagatt ttacaggcgc tacatctgca ggcgtatcaa
     2701 ttctggatac ggcaacaggg aatgccgcca aattcagcgg ttccagcctt ctgattaaag
     2761 cactgagtga gcaaggcgat gtcagcgttg taacttcaca agaaagcacg gtcacaaacc
     2821 tgacgccggt acctatccag atggcagatc agacagttta cgtcgcccag tcagcaacaa
     2881 ctacgactac ggatgtagga gcaacaacaa cattaacacc gggcatgatc accactggat
     2941 tcaatatgac cctgctgcct ttaattcaga aaacaggcaa tctccagttg cagatgaact
     3001 ttaatctgtc tgatccccca actatccgta gctttacgtc aaaagacgga aacagttaca
     3061 tcgaaatgcc atataccaaa ctgcgttcac tgagccagaa ggtcaatctg aaagaagggc
     3121 aatcacttgt cgttactggt ttcgatcaga acaatacgac gacaagtaaa gccggtacgt
     3181 ttacgccagc aaatccatta tttggtggtt cacaaaccgg gaaaaatgaa cgcagcacgc
     3241 ttgtgatcat cattaccccg actttcccgt caggaggcaa taatggctga tgaagatatc
     3301 aacccggtag tgcttctcgc agatcctaaa gtaaatcatc gcgtgtgggc tgcctgtctt
     3361 aaatggactc ctgtcgtaaa aaaacagcgc gtcccctcac atcataaaca caagtctcat
     3421 gtaaaaccca gacggcttac ctccctgaaa gtgactgtag gcagcagaaa ttcccgtgga
     3481 gagatcagcc gccttaccgg cacaggaata ctgaccaggc cagaacgcaa tcattatttt
     3541 tccctggcac tggctttctg ctcatgggtc agaaatggat acggtgtctt ccggtacagc
     3601 gataaggagc tcttattcct tgcttccatt aatggccagc ctgccgttat ggctgactta
     3661 tcaggaaatg atgccgacgt tgcccaaaag gtcagccttt ttctggcgat gaacgaagag
     3721 ccccccgaaa aatggcaggt tgtatcgtct ctggaacatc ctgataactg ggaaagtatt
     3781 attacccggt tatcatcagc agacctccgc cgttgcaaac ttactgtcgg caatcgcagc
     3841 aaattcacac ttcctgccgt actgttttta gtagcagccg gcgcaggcac agtattctgg
     3901 atgactcagc cagagcctga tgtaggaccg accgccgagg agatagctgc acgggcaaga
     3961 ttacagttta aaaagcccga accaccaccg gagctcccgc atccatgggc gtcacaacct
     4021 gtaatcagtg attttctgaa agcctgcgcg gaccttcgaa aaccatcacc ggtcgcgcta
     4081 gaaggctgga aacttaccgg tggaacatgt actcccgaga cattcacact gatttacgaa
     4141 cgacagccag gaggaactat tgaaggcttt ctggcaagaa gcaaagaaat ctttaatgtt
     4201 attcctgact ttaaccttaa agatggtgca cgcctggctt ctgtaacccg gccactgcct
     4261 tccctgcccc gcagggatga ggcagtccct gccccctcag aacaactgat gcgggtgttc
     4321 acgtggtttc agaaaaaaca gttaacgccg gccattaatg aaatagccat acaggaacca
     4381 ttaccaggta acgatggtga accagcccct gttcagaaat ggaaagaata tcagttcagt
     4441 ttatcaacgc ctgtaaatcc tgatgagttg ttccctttat ttcaggatac cggcgttcgt
     4501 ctgagcaata tccattttga attaaacggc ggtactttca gttattcctc agaaggtcat
     4561 atatatgcgt ccaggtaaat tactcattat tccatccata tttttcttct ctggtttttc
     4621 atttgcaaca acacaacctc ttgtcacaat aggagaactt gaagcacaac aaaaccggaa
     4681 tattctttta caggcaaaag ttcagggcgc acagctacag aaacaactcg aagaatcaga
     4741 tgttaatctg tcatcagaaa ctgtttcagg tttctctggt gtgactgcct ccttgccatc
     4801 agtttcagag cagccgacat ctaaaaacag aaaggaattg cccgttatta tggaaataaa
     4861 cggcaaagat aaacgtcttc atgccgttct tcgcatggct gatggacgtc agacaagcgt
     4921 caccactggt agccagcttc cgggtacatc cgtcacggtg aagtcgatct cattgtctgg
     4981 cgtcacgtta agtgatggca ccacccttac tttctgagtg atgtgatatg gacaaatata
     5041 atctgaagga tgctctcctt tttatatccc ggggagacac ccatgaaatt ctcatcgaaa
     5101 caaatcagcg aacaagacct gacgtacaga gtaatctcca ggaactgcta aagttatacc
     5161 ctgacatcac cccaaaagta gtctcgctgt ctgagttaca ggaagccggt caggatgatg
     5221 aaaataaagg tccgaaagaa cggattattc atctgaaaga tcttgcagat gtcagtgaaa
     5281 gtgagaagaa agttctctcc tattttgaaa cggccagaaa acttggtgct tcggatattc
     5341 attttttaat ttcagagtct attttcaaag ttcgcatgag aatattcggt gaactgcaga
     5401 ctgtagatga agatcaacct gcattgggtt attcactttg cgcaacagcc attctgtcaa
     5461 tggctgatgt cactgaaacc agtttcttcc ctcaaaggga acaggatgcg cgcctgtccc
     5521 cacaattaat gcgaaaaata ggaatatttg gtgcacgata cagtcacaga cctacgggag
     5581 atggtctgat tgcggttatg cgcctgatac ccgatgacgg agacaaagtt cccacgttta
     5641 agcaactggg ctttataccg gagcaaatcc ggttgctgaa cgtcatgctg cgccgcccgg
     5701 aagggaaaat tgttctgtct ggtccaacag gttcgggtaa atcaacaacg ttacgcagtg
     5761 cctgccgtgt atatcttgac gataatcagg ggcgacacct gctgacgatc gaagatccgc
     5821 tggaaggaca gatactgggg gcaacacaaa ccccgattat ctgtgacaaa tccgacgaag
     5881 aggccgtcaa actggcatgg agtcgtgcta tttcatcggc aatgcgactt gaccccgatg
     5941 ccatcatgga aggggaaatg cgcgatcttg tctcgatgat gtcaaccacc tacgcggctc
     6001 agaccggtca catcgttctg acgacattac atacaaactc agctctgggt ataccggagc
     6061 gaatgattac gatgggtatg aatgccgatc ttatttgtga tgcgcaatta ctgattggga
     6121 tgataagcca gcgccttgta ccaacgctgt gtccctcctg ccgtattcca tgggaaaagc
     6181 gagtacctga acttagcgaa gacgaccgcg cttaccttga gcggcactgc aataaagatt
     6241 cactctgcag tacagataat atctggttcc gaaatcctca cggatgctcc gactgtaatc
     6301 acgacgtaat tattaacggc agaaagcgcg gtgaaatagg taaaggcctc accggcagga
     6361 ccgtaattgc tgaagtgata gaacctgata atcgcttatt tcagattctg aaaacacgcg
     6421 gcaaagttgc agcacgtaaa tactggcttg aaaacatgca tggcatcagt cgtgtggagc
     6481 atctgcttcg ccgtatcaat gaaggcctgg tggaccctct tgaagccgac agaatcatcc
     6541 ctctggacga agacgaacgc ctcagcatcg atgatgccta acaggagaac aacatgcgtg
     6601 aaatgaattt cagtcaacgg ctgagacgtt tcattgtcag aaaaacgttc agcgccccct
     6661 acagggtgca gttttacgaa gctctgcgtt ttctgctgga aaataaacag ccattaaaga
     6721 ccgcactgga acagatgcgg gatgcctgga cagactttgg acgaaaatgg cacccctttg
     6781 ctgaactggc cacagactgt attgagtctc ttcgtgaaaa cagtggcgaa aactctctgg
     6841 aatatactct tggtttatgg gttccccagg aagaggctgc agtaatcagt gccggaataa
     6901 gaagtggttc cattgtggat gcattacaat ttgccaccac gcttacagat gccaaaaagc
     6961 aactccatca ggccatatgg caaatggcta tttacccggt gggattgtta atcatgatga
     7021 cggggacatt atatgttctt aatacagagc taatacctgt actgagcaaa ataagctccc
     7081 ctgattcatg gagtggtgct cttggttttc tctatggatt atctttgttt gtcgataatt
     7141 acggcgctat atgtgccgtt ctttttgcag tggtcacagg tgtaatatca tggtctctga
     7201 aaaactggaa aagccgctct ttggctgaca atataatgcc atggagtatt tataaggata
     7261 tacagggggc agcattcctc ctgaatatgg ctgcacttct gaaagccaaa atgacaacgc
     7321 tcaactcact gaacgttctt caggaatttg catcaccatg gttaagtaca cgccttgaca
     7381 gcattattta ccgcgtccgc cagggggatc accttgggct ggctttacga cagtgcggtt
     7441 atcatttccc ttccagggag tctgccaact ttctgtcttt attacaggga gatggtgcaa
     7501 cggagctaat cggcaattat ggccaacggt ggcttgtaca aacgctggag cgtgtaaaaa
     7561 aacgagcagc tgttgtgcgt ttaattatgc tgatatttct tgtcatgtca ctcctgttac
     7621 tggtcatggc cgttgtagac attcagtcta ttggcgacaa cagtatgggt agtatttaaa
     7681 taaaacgagg tatttatgtt aattcaaaat attaatgcta acgttctgaa gaaaaatgag
     7741 aaagaaacgt ctcatgacaa aggctggggt attatggaac agggtgcaat agcacttgtt
     7801 gtaattatcg tacttgccct tgtttttggt ggattgtata tgttgcgttc acgaaccggt
     7861 gtggcaaatg aaagttcgaa catacagaca atcatcacaa gtactcaggg gctgctaaaa
     7921 ggtagcgatg gatatacgtt taccagtgga gccaaaatga cgggtgctct cattcagatg
     7981 ggtggcgtac ccaagaccat gacagtgcgt ggtactccat catcaggtac agcaacgctc
     8041 tataactcat ggggcggtgc cgtaactgtt gctccggctt caacatcagg tttcaacaat
     8101 ggttttaccg tcacctatga aaaagtccca caggatgcct gtatccagat tgccacacaa
     8161 atcagtcgta ctggtttaac taacggtatt accctgaaca gtacggctca cagtgatgga
     8221 aaagtcacta ctgaagaagc cagtgcgcaa tgcacagctg ataatggcag caccggtaca
     8281 aacaaactaa tcttcaccat caatggctaa ggagttttaa tgtccggggg taaagtgccc
     8341 ctcagactta tttcattaat cctgctttgt tctcttattt atcccgcccg agcttcatac
     8401 atcgccccca aacaaatctg gactaacaag tgggacaatt gctttgccgc cgccggcgca
     8461 cgctatcaaa ttgaaccatt attgttgaag gccatttctg ccggggaatc ttcattgaag
     8521 ccgggcgcaa ttaacataaa taaggacaga aaaacaggca aagccagtag tacggattat
     8581 gggttgatgc aaataaactc aacccatatt ccaaagctta taaagatggg cgtaatcaaa
     8641 aagtctgagg atttaattac caaaccatgt cttaatatcc atataggaag ctggatactg
     8701 gcaagacatt ttcagatatg tggcgtcagc tggaattgcc ttgggtcata caatgccgga
     8761 ttccgtaagg acagacatga aacaagagag caatatgcta ataaagtatg gagaatttat
     8821 cgtgacatga aagggatatg ccttcccggg cagggaggca gacaatgcag acaatcgtag
     8881 gcataagtct ctcccctgtt tatatcctcc cattgatgtt catattttcc attattggac
     8941 gaactctgtt attcagagtt cgttattttt tatctgatac agaacatctc tggtataaga
     9001 cacacactgc agttctgtca ggaatatggc tatatagtat agctgtggca ttaattattt
     9061 tatcgtcatc cccacttatc taccgtattc atgctgttct tattctttcg ttcatgctgc
     9121 aaatggcagt aacggatgca ctaacaggcc tcttaccggg tacattcacc aggcgttttt
     9181 taatcgctgg aatattatca caaatcacaa ctgatatatg gtggctccgt gcaacagaaa
     9241 ttgtctctgc cgcaattgtc ttattctgtc tgcataaact ggttaaccgc catcgtctta
     9301 atattggcac aggagacctg tggcttattg caggtattac cgcatggtca gggctatata
     9361 acgccatatg gagtgtgttg ctgggcgcag gaggatttgt cttatggcat tcaacatggt
     9421 gcataaaagg acataaagaa ggcccactgg gtccatggct gtgtcttggc catgtccttt
     9481 tgctactgga taatctttat caaccactat gggtaatctg atgaaaaaat atgaccgagg
     9541 ctgggcatcg ctggaaattg gcgcggcttt attgattgtt atgatcatag ttgcctgggg
     9601 tgcaggcata tggcaggact atttaaaaac caaaggttgg caggctgaag ctcgtcttgt
     9661 cagcaactgg gccagtgcag cccgctctta tatagggaaa aactacacaa ctctccaggc
     9721 cagtagtacc acaacaaccc ctgccgttat cacgacaacc atgctgaaaa ataccggctt
     9781 tttgtccagc ggctttactg agacaaacag cgaggggcag cggttacagg catatgtagt
     9841 tcgaaacacc caaaacccgg aattactaca ggcaatggtt gtatccagtg gtggcacccc
     9901 ttatccagtg aaagcactta tccagatggc taaggatatt accactggtc ttggtggata
     9961 tatccaggac ggcaaaacag ccacaggtgc attacgttcc tggtcagtag ctttaagtaa
    10021 ttatggtgcc aaaagcggta acgggcatat tgccgtactg ttatcgacag atgaacttag
    10081 tggtgcagca gaggacactg atcgtcttta cagattccag gtcaatggtc gccctgactt
    10141 aaacaaaatg cacacggcca ttgatatggg atcaaataac ctgaataaca ttggggcagt
    10201 aaatgcccag actggtaatt tcagcggcaa tgtgaatggt gtaaatggca ctttcagcgg
    10261 tcaggtaaaa ggcaatagcg gaaactttga cgtaaatgtc accgctggcg gtgatatcag
    10321 aagtaataat ggttggttaa ttactcgtaa cagcaaaggc tggctcaatg aaactcacgg
    10381 tggcggattt tatatgtccg acggatcatg ggttcgaagt gtaaacaaca agggcatcta
    10441 taccggcggt caggtgaaag gcggtactgt tcgtgctgat ggtcgccttt atactggtga
    10501 atacttacaa ctggaaagaa ctgccgttgc tggcgcatca tgttcgccta acggccttgt
    10561 aggccgcgat aatacagggg caatactttc gtgccaatcc ggtacgtgga ggacaatagg
    10621 tggaaaactc aaagttactc agctttccac cacaggttat ctggggcaat tcgacttctg
    10681 tgccattgcc agagtgggca acgcagagga tgcccactac tgccaggtag ttgagagccc
    10741 agcaggttca cgtaaatggt acaaatacga gcataagaca ggttgtattg cgtcgtgtgt
    10801 aacgctcaat taagtttgat atccaaatac tgaaaatacc ccggcaccac atgatgtatt
    10861 ttctgttgga taggatgtta tctgatagga agtacctgca ggtacagcaa agctgataaa
    10921 ggctgttttt ccatatgcag ggttattgct ggcattcacg ctaattagcg tcccaccaac
    10981 atatccctgc agtcgggatg tatttacaca tgcacctccg gcagatcctc cattacctcc
    11041 agatgcataa ataaacaatg tactgccccc tgaattccgc cctgagaatg aacctctatg
    11101 tgaacctaag tttgtgtaag aaccattgag cgaaccagaa gtcttccacg taccggattg
    11161 gcagtaggcc
//
LOCUS       DAFNBJ010000153        11137 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000153 DAFNBJ010000000
VERSION     DAFNBJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.153"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..770)
                     /locus_tag="L2X49_003915"
     CDS             complement(<1..770)
                     /locus_tag="L2X49_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone of endosialidase"
                     /protein_id="HBN4798928.1"
                     /translation="MWGCHNGTGWLPLAVGQGGTGATTVEDARNNLSLGESSAVKFKN
                     LTLTEALDTTLGLLTKTGRDWNTQHTDNINKFIPIAGSTNGPAGSMVLGGIHVQFSKN
                     YAVQFGGRNSGFWGRTIENGTTQEWKKLLTVDDLNSSTDLAVRSLTTSNPVKSGGGRI
                     DVLGSTSDYSKMDCFVRGFDSTGNSLVWALGSSVGVSKMLSLKNFFSGAEILLNGNDG
                     AVQLKTGAVNGATAQTLTINKNEVNSTVDLTLTKQSGTG"
     gene            complement(1184..1531)
                     /locus_tag="L2X49_003916"
                     /pseudo
     CDS             complement(1184..1531)
                     /locus_tag="L2X49_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            complement(1602..3617)
                     /locus_tag="L2X49_003917"
     CDS             complement(1602..3617)
                     /locus_tag="L2X49_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001631326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBN4798929.1"
                     /translation="MLAVNSQTRTLTLDREITLPSSGTTLISLVDGQGSPVSVEVQSV
                     TDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKE
                     AIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLL
                     RLTVAADDGRERLVSTARTTETTYRFTQLALGNYRLTVRAVNARGQQGDPASVSFRIA
                     APAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETSARYLGTAL
                     YWIAASINIKPGHDYYFYVRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLA
                     QELWTQIDNGQLAPDLTEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNN
                     LNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAMINPANGNTKPMF
                     VGQGDQIFMNEVFLKRLTAPTITSGGNPPAFSLTSDGRLTAKNADISGSVNANSGTLN
                     NVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNGTVTVTISDDHNF
                     DRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYS
                     AVIDMPSGRGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            complement(3636..3764)
                     /locus_tag="L2X49_003918"
                     /pseudo
     CDS             complement(3636..3764)
                     /locus_tag="L2X49_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
     gene            complement(4057..4131)
                     /locus_tag="L2X49_003919"
     tRNA            complement(4057..4131)
                     /locus_tag="L2X49_003919"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4096..4098),aa:Gly,seq:tcc)
     gene            complement(4135..4210)
                     /locus_tag="L2X49_003920"
     tRNA            complement(4135..4210)
                     /locus_tag="L2X49_003920"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4175..4177),aa:Thr,seq:tgt)
     gene            complement(4291..4366)
                     /locus_tag="L2X49_003921"
     tRNA            complement(4291..4366)
                     /locus_tag="L2X49_003921"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4331..4333),aa:Ile,seq:cat)
     gene            complement(4510..5331)
                     /locus_tag="L2X49_003922"
     CDS             complement(4510..5331)
                     /locus_tag="L2X49_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBN4798930.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(5328..5702)
                     /locus_tag="L2X49_003923"
     CDS             complement(5328..5702)
                     /locus_tag="L2X49_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBN4798931.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            complement(5715..6764)
                     /locus_tag="L2X49_003924"
     CDS             complement(5715..6764)
                     /locus_tag="L2X49_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBN4798932.1"
                     /translation="MRVLLRPVLVPELGLVVVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRGSLGLRAEKIRSMYRESDIVPGEQTANSILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(6766..7044)
                     /locus_tag="L2X49_003925"
     CDS             complement(6766..7044)
                     /locus_tag="L2X49_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798933.1"
                     /translation="MARNVKYYNSDNSPVLACTHERYSHAFKSEWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(7111..7362)
                     /locus_tag="L2X49_003926"
     CDS             complement(7111..7362)
                     /locus_tag="L2X49_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798934.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
     gene            complement(7578..7733)
                     /gene="hokD"
                     /locus_tag="L2X49_003927"
     CDS             complement(7578..7733)
                     /gene="hokD"
                     /locus_tag="L2X49_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBN4798935.1"
                     /translation="MKQQKAMLIALIVICLTVIMTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(8462..9442)
                     /locus_tag="L2X49_003928"
     CDS             complement(8462..9442)
                     /locus_tag="L2X49_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012846940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798936.1"
                     /translation="MKFKDKNLKALAECIIGDNKAFLYRSSSHITEFFQDCGMDVTHD
                     GSTRWKWTAQRLEELLYEPQSKPHTLPERFVHVLRTLMLKEDAMDDDPGRLKALEELN
                     KPLMREGYEAFYGDDRLLYIRHTDTKTVSVSNNPHRPLTPHEVECRRLLTAFLDTCSE
                     DELIEDILLPLFRQLGFHRITAVGHKDKALEYGKDIWMKFTLPTQHVLYFGIQAKKGK
                     LDASGASKSTNSNVAEIFNQVLMMLGHEIFDPETNRKVLVDHAFIVAGGEITKQARNW
                     LGGKLDASKRSQIIFMDREDILNLYTVSNVPLPTGALISDDAVKNDDIPF"
     gene            complement(9802..10404)
                     /locus_tag="L2X49_003929"
     CDS             complement(9802..10404)
                     /locus_tag="L2X49_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN4798937.1"
                     /translation="MNTSPWNKDRIIGQKRPLQISHIWGIRIRLELEGKTRDLALFNM
                     ALDSKLRGCDLVKLKVSDVAYGGSVSSRATVLQQKTGSPVQFEITKGTREAVAALIQL
                     SNLHSKDFLFRSRVGTNQHISTRQYNRIFHGGVEKLGLEDSLYSTHSMRRTKPYLIYK
                     KTKNLRVIQLLLGHKKLESTVRYLGIEVDDALEISESIEV"
     gene            10722..>11137
                     /locus_tag="L2X49_003930"
     CDS             10722..>11137
                     /locus_tag="L2X49_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798938.1"
                     /translation="MNAGLHFSEISKEQFDIYFYGRSPYLKTFSEEIRWFKYEGNGIT
                     LLSTIIICNIDKDFNAIVLGRDLDKKFRAINVLASFDSMDVLLNNLNDDIPKMLAQHQ
                     NGTFMQGDESTKPFSLFLSKVPAKKRNVYIKMLLEDP"
BASE COUNT         2757 a         2898 c         2493 g         2989 t
ORIGIN      
        1 ccagtccctg attgctttgt aagggttaaa tcaacggttg agttaacctc attcttgttg
       61 atagtgagcg tctgcgctgt agccccgtta acagcacctg ttttgagttg aaccgcgccg
      121 tcattaccat ttaacagtat ctcagctccg ctaaagaaat tttttagcga tagcatctta
      181 cttacgccga ctgatgaacc caacgcccac acgagagaat taccggtgct atcaaaccca
      241 cgtacaaagc aatccatttt gctatagtct gacgtgcttc caaggacatc aattcgccct
      301 ccgccagatt ttaccgggtt agatgtggtt aatgacctga cagcaagatc ggtagatgaa
      361 ttgagatcgt ctactgttag taatttcttc cattcctgtg tcgttccatt ttcaattgtt
      421 cttccccaaa aaccggaatt gcggcctccg aactgcacag cataattttt actaaattga
      481 acatgaatgc cgccaagaac catagagcct gccgggccgt ttgtactgcc tgcaattggt
      541 ataaatttat taatgttatc agtatgctgc gtgttccagt ctcgtcctgt ttttgtaagc
      601 agtcctaatg tcgtgtcgag cgcttcggtt aaagtaaggt ttttaaattt aactgcgcta
      661 ctttcgccaa gacttaagtt gtttcgcgca tcttctactg ttgtcgcacc tgtacctcct
      721 tgcccaacag ctaatggcaa ccagccagtg ccattatgac aaccccacaa accagatttg
      781 gaaacctgta agcgtggtgc gcctgaagaa tacgtagaat atacgtaagt tgtttctttt
      841 ccctcttcaa ccctttctgt tgatacatct ctcttccatt cagaccaggt accgctacct
      901 ccttcatata ttcgtctata agtaattccc ggattattat aagggtggta cacctgaacg
      961 caggaagaag cagtgttggc tctcgtttgg agcactatta aagcgcccgc cagaccgatt
     1021 gggtaattta actcttctgt tgcgtaagcg ctcgttggtt gttgatagaa accagaatat
     1081 tcaccggtaa gggtgtttag gtcagtatta gcaaggccag ctttttgctc atacattacc
     1141 cgcaagttcg cacgtgcttg ctcgatagtt cttccaccag taccatcgca tacgcgctga
     1201 accattcatt cacgcgcaca gacggccccg ccatcacgct gaaccagcgg ttacgcacgg
     1261 aatcttcatg ccagcgggta tcgctgtaac gggtcagctg gcgattcttg tctcctgcat
     1321 agctgaatga cgtcaccagc cccagcgtat ccgtaaactc ataacgatat ttcacgttaa
     1381 tcccgttcag atcatcactg ccgggaacgt tggtccgggc atgaagatac cccgcgctca
     1441 gcgtggactg atgttcagac gcccatgcag gcgcaccgga tacggccaga caaatggctg
     1501 cggacaaaat tgctgcacaa actttacgca taattacctc tcgcttttct gcaataaaaa
     1561 aggcgtcatt cctgacgccc tttattgggg ttataaatat ttcaacgaat actgatgccg
     1621 gaagccgctt ttttggtcac aatcaccgta cagtcggtga tattgcctgc cccctgattg
     1681 cctttctgga aaatcttaaa ctccagagtg acgcttcccc tgccactcgg catatcaata
     1741 actgcactgt aactaccggg aatggcccct ttagtttctc tggatgcgat taatacgccg
     1801 tttttgcgaa cttcaaaacc ataacccgtg tatcgcgtgc ctcctgggtt atttccgctc
     1861 cccggatcgt catacgctat accgttaaaa ataatgggcg gaataataat ctggcggtca
     1921 aagttatgat catcgctgat ggttactgta accgtaccgt ttggtgtttc cgtgttaccc
     1981 cacgtaccga cttttttcgg gaaggctttt gatacagctt taacgaaatc tcctctgacc
     2041 tgggtcgcct ccagcatgcc cttaatcgta cagttctggt taatcgtgac attgttgagc
     2101 gttcctgagt tcgcattcac actgccactg atatccgcat ttttcgccgt cagtctcccg
     2161 tctgatgtca gggaaaatgc cggaggatta ccgccgctgg taatggtggg ggccgtcagg
     2221 cgtttcagga acacttcatt catgaatatc tgatcgccct gaccaacaaa catcggcttt
     2281 gtgttgccat tcgcaggatt aatcatcgca atcctgtctg ctgccagcag cacctgactc
     2341 tgcatgccgt caggggtgtt ctcaataccg gcaccgatac ccgcaatata aaggcgtccg
     2401 tcctgcatct gctgcagctt cactgcccac atgctgttca ggttattatt tgtatcaacc
     2461 tgaaccttct gtatctgctg gatcgctgca ctctggtctt ccagtttctt attgacggtc
     2521 tgtgttattt cattgctgac atccgttatg gacgtcctga tttcagtcag gtcaggcgca
     2581 agctgaccgt tatcaatctg cgtccacagc tcctgagcca gatgggtttt ccctatctcg
     2641 cctttgaaaa aatccagata gcctgatgca tcatcactcg gctgaccgac agcctccacg
     2701 aatgccgatt tgccaacggt gttcacactg cgaacgtaaa aataataatc atggcccggt
     2761 ttgatattga tactggcggc tatccagtac agtgccgtac caagataacg cgcgctggtt
     2821 tcaacctgcc tgatatccgc aatccgtttt tccgagaacc agaactcaaa ctgtaccgtc
     2881 gggtcataaa ccgcaagatg cggcgtggcg gttatctgaa aatagcccgg cgtcagctca
     2941 atccgcgacg gcgctgccgg tgcggcaatc cggaacgata ccgacgccgg atcgccctgc
     3001 tgcccccgcg catttactgc ccggactgtc agcctgtagt tccccagagc cagttgtgtg
     3061 aagcggtaag tggtttccgt cgtccgggcc gtgctgacca gccgctcacg gccgtcatcc
     3121 gctgccacgg tcaggcgaag caggaagctc acgcccttca ccaccttcgg cgtgtcccag
     3181 cgggccagca cctggtattc cccgctgtct gcggtgactt ctgcggtcag gtgctgcacc
     3241 gctggcggcg tgacaccatt taccgtgccg ctctggtcgc cgtcaaagtg cgccccgtta
     3301 tccacgatgg cctctttttc cggtacatgc tgcacggcgg tgatggcata cgtgccgtcg
     3361 tcgttctcac ggatactcac gcagcggaac aggcgctggc gcagcgtcgg cagcttcagc
     3421 ccccatacgc tgtattcagc aacgccgtca ggaacacggc tcactttcac cttcacgccg
     3481 tcggtgacgg actgaacctc cacgctgacc ggactcccct gcccgtcaac caggcttatc
     3541 agcgtggtgc cggaagatgg cagcgtgatt tcacggtcga gcgtcagcgt ccgggtctgg
     3601 ctgtttaccg ccagcacgcg cccgccggtg cggataccca gcacacctat tgctgcccac
     3661 tgtgagggcg tgactttatc gagcagctgt aaaaaccagt agccagcact gcctgcggag
     3721 gtgccatagg caatgcccgt tgaaattttg tccatggatt tcatagcctc acctccgcaa
     3781 ataacggatg gcgtagtttt acactgagaa atgaaaggga tttgaaaaga aaagcccgca
     3841 aaagcgggcg aaacaatata tacagtaagg aaagcactct atccaacaaa ccacccacag
     3901 ttaatcggaa taaaagcaga gtgcttatga atgatcgcct gcccgaaggt tagtatttct
     3961 gcacagcaat tttgcaaaaa aaagcgatca ttcataactt aaacgtcttt cagtcactcc
     4021 gggatttccc atcatcgcag actgaaagac tctaactgga gcgggcagcg ggaatcgaac
     4081 ccgcatcatc agcttggaag gctgaggtaa tagccattat gcgatgcccg catatggtgc
     4141 cgactaccgg aatcgaactg gtgacctact gattacaagt cagttgctct acctactgag
     4201 cgaagtcggc actggaccgc caccggggac tcgaacctcg cacactcaac ttaaagggtt
     4261 gacgctcttt cctgatgaac tggtgacggt tggtggccct tgctggattt gacccagcga
     4321 cctgtcgatt atgagtcgct cgctctcacc actgagctaa agggccggga gcagaataat
     4381 aacggtccat aattaattcc gcaataaaaa acccgctcgg cggcgggttg tagaaactct
     4441 tctaacgtca ggcataaaac gcccatcgtt atgacgaatt taccacagat tccggaaaaa
     4501 tcaaccttgt tacctagtta ccttttttaa ctgccgctca gcccatgctt cttcaatatc
     4561 aaaccgggtc accagcgcat catagaattt cttaactgtt ttttcccatg acgcgcgtgt
     4621 tatctggttt gtcacctcgc atatagcatt aaatgcctcc gttgatggta gtctttcata
     4681 gccacgacca ccacaacgct ggcagtctct gataacaggc ataccacgtt ttaccgactc
     4741 ttcacgatga atggcgacac cacgcccacg gcaatcctta caggcggtgg aaacctcacc
     4801 ctttccgcca cactccggac aggcaacttt taccacctcc ctgacttttt tccattcttc
     4861 ccagtaagac ggatacacac ctttcgtaca ctttgcccat accggcggct taccatccgg
     4921 atactggacc ttgtttgtaa aaactacgct ttcaataaat ttttccccat agcaacaagg
     4981 gcactgcttt ttactcgctg cgctgcgggc ataatcctca aaagcgtacg aagccataat
     5041 gcgcatcact accggtttta tttctgccgg aagctttctc aacgccgcca cacgatcgca
     5101 ccgactgagt gcataatctg ccagtaattc tgttgcccgc gccctgtcat tcatactgat
     5161 gcccattttc cccaggaacg cagaaaaacc catctcagcc cgattctgtg tcatgccctg
     5221 cgcggccatc acatcagtga tactcagcgc atcttttgac gttgaggccg atgcatcggt
     5281 caggccaggg gattttgggg agtagtattt cggtaaatct tccagtttca ttttttgacc
     5341 tgctcttcat gcattatggg gtaaatcttc acccccagac gtccaccaga tactggctga
     5401 ccacgaacga tattgatttc atcaaactgc tcatcgtcca ttaacactcc cgcatgcgtc
     5461 agcgcatcca gcggtgcttt caggatattg tccaggtcgc gacgacgctt atccggtggc
     5521 tctgcaatca cctttatcgc cagccttccg gacaggctta atttcagccg ctgctggcga
     5581 acaataagcg ccacagcccg gcgataacgc tttccctcct ccgagataaa atatgtgctg
     5641 ccacggcgtc gccagtaagt gttcaccgtc ggcgggtaag gtaaaaccaa atctatgagc
     5701 atcagtcacc tcttttaccc aagcacgcca gttgcaaagg cgtgatcaag aaaacgaaaa
     5761 attaaatcaa cctgagaacc atgcttttct tcgaacgcca gaggatccgc atgaagctcg
     5821 ttgtgatgct cccgacacag cggtagcgtg aaaatatcgt gagattttgt ccccattccg
     5881 ccctgaccat gaccaatcag gtgatgggga tcgtcggctg gcttaccaca acacgcacac
     5941 ggctgtgtct tcacccagcg tgtgtatttc tcgttaaccc agcggcgacg tttaggtcgt
     6001 ttcatgaaag attccggaga ctcaggatca acggcaatgc tgaccaccgt cttttcctgt
     6061 ggtgggttct gttgctggtg ggcgtgaggc agcggcgcaa gattttttgt gcgctgtttc
     6121 agtatgctgt tggcggtctg ctctcccggt acgatgtcgc tttcacggta cattgagcgg
     6181 attttttccg cacgcaaccc cagcgaacca cgtaataccg cttccggtag cgcgtccgcc
     6241 acctgattgc ggaccgccca ccaggataat tcagccaaag ataattcacg ctcctgcgta
     6301 ccgcttattg cgtgaccgat gacgtcaatc atccatgctg acaggttttg atgagcaagt
     6361 tgctcgagtg attcggatgt ctggtcacgc agctggttgt cgcagtgcca gcacaacacc
     6421 attgcgccgg taccataacg gtgaatgacg gtttcgctgt gatgataatc gccgtgtggc
     6481 cactggcagg atttaatatg gcgcaacagc cagtcagaca atgcaccagc accaccagca
     6541 gcacgaatta cccgtgcgtt actgaaaaac ggcagcaatg ttttgtcttc caccagcggc
     6601 tggcgaacgg caggaacgac cccggacggc agattacgca tgcttttcgg ttccggctcc
     6661 accagtaccc gggtattgtg gaataccggc atggattcac ggcccggctt aacgaccacc
     6721 agcccgagtt ccggtaccag aacaggtcga agtaataccc gcacgttacc tccagatgcg
     6781 ttgctggaat gtacgggacg gacgcggtgg gcgttcggag taaggcaatc tgactgagat
     6841 tatccagtga cggtagtcga gactaagagc tttcttaacc tcgtatccgc gcctgcggta
     6901 acactgaatt atccattcag cctgctcttc agtgcatgga gggtgctgga accattcaga
     6961 cttgaatgcg tgagaatacc gctcgtgcgt gcaggcaaga acgggcgaat tatcagaatt
     7021 gtaatatttt acgttgcgtg ccatcggttt tctccggtgg cacggtgtta ctcagcggga
     7081 gttcagcccc gcgcaagatt gtagatgagt ttattcttct gcaaaagctg aaaagcctgc
     7141 ttttattcca atctctttca gtgcctgtaa tgaagtgaca aactcacctt cgcgcaagat
     7201 aaatccgtct gtcactcgac catccacaaa attaattaac gcagccccat tctttcgcaa
     7261 acacataatg cggtaatgac taacaagatt tccattttca acgcacacag catagaggcc
     7321 atcttcacaa aaaattttac gcagttcttc gatgttcatc atcagaatcc ttccggataa
     7381 ttagctctcc cctttaaggg accatccctc ttatccctgc gcgctactta agtatttttg
     7441 attctattcc ggcaccgtcc agaacttcaa acgcgttgaa aataaaaaca aaaacccgcc
     7501 gaagcgggtt aagtgcgggt gcgttgagga tgcctgacac atcagaggtg gcgagggatt
     7561 ctcccccgcc tggtctctta ctcctcaggt tcgtaagctg tgaagacagc gacctccgtc
     7621 tggccggttc ggattcgtac ctcgcagagg tctttcctcg ttaccagtgc cgtcattatg
     7681 acggttaaac agatgacgat cagggcgatt aacatcgcct tttgctgctt catagcctgc
     7741 ttctccttgc ctttcggcac gtaagaggct aacctagatt tgccgttcat agattgagcc
     7801 tcagattaat gttaaacgtc ttgcaggacg cgtaatgtta actggggctt ttctctatct
     7861 gcctttggtg ttcatgcctg aggcagatag cctcaagcac ccgcagcaat tctacttaac
     7921 tcccgtcacc tcgccaatat aaaatcaatc agaaaggcga tccgtaagaa caatagcaag
     7981 acaataaatt gccattacag ccgcaatagc tagcgcatat ttgagaacca gcacgacaac
     8041 ctcctgtatt ggacgtacac cagttctgat aaatatgagg ctgtctcatc attgattcaa
     8101 tataactatt gggtatagtt tctgtgattt tgttttgtag aaatggaaca caacaaccag
     8161 ttaccaccag cacttcttta aatacgccaa gtccgacgca agctaacctt ctagtccgct
     8221 ttgagcgaga aacggaagtt ggtttgctgg tagcaatcta aaagtgaatg gtccggttaa
     8281 gttgggcgtt tacagttact cggcaaacta ctccagcttg atgaataagt atttttaata
     8341 aatttcagcc cgcgatgccg cggacattca tacgggctgt tatattacca gattaagggg
     8401 tcactaaagg tgtggtgtgg ctacctatca cgtattcttt cagaaaaaga cgtacttctg
     8461 attagaaagg aatatcatcg ttcttaacgg catcatcaga gatgagagca cctgttggca
     8521 gaggtacatt acttacagta tataaattaa gaatgtcttc ccggtccata aatattatct
     8581 ggcttctttt gctggcatca agtttcccgc ccagccagtt cctcgcctgt ttagtaattt
     8641 ctccgccagc aacgataaag gcatgatcta ccagcacctt tctatttgtt tctgggtcaa
     8701 atatttcatg gccaagcatc atcagtacct ggttgaagat ttctgccacg tttgaattcg
     8761 tagatttgct ggcaccggac gcatccaact tacctttttt tgcctgaatg ccgaaataaa
     8821 gaacatgctg agttggcagt gtgaacttca tccagatgtc tttcccgtat tccagcgctt
     8881 tatctttatg tcccactgct gttatccggt gaaaaccaag ttgccggaat aaaggaagga
     8941 gaatatcttc tattaactca tcttctgagc aggtatcaag aaacgcggtc agtaaccttc
     9001 tgcattctac ttcgtgaggc gttaagggcc gatgagggtt attactgact gaaaccgttt
     9061 tggtatcggt atggcgtata tacaaaaggc gatcgtcacc atagaatgcc tcatagcctt
     9121 cccgcatcaa aggcttgttc agttcttcaa gcgcctttaa tcttcctgga tcgtcatcca
     9181 ttgcatcttc ttttaacatt aaagttctga gcacatgaac aaacctttcc ggcaaagtat
     9241 gtggctttga ctgtggctca taaagaagtt cttcaagcct ctgggccgtc catttccacc
     9301 gagtggatcc gtcatgagta acatccatgc cgcagtcctg gaaaaattca gtgatgtgac
     9361 tgcttgaacg atacagaaat gccttattat ctcctatgat acattccgcg agagccttaa
     9421 ggttcttgtc cttaaatttc atgtgtaata atctccccct cgtttagaat atagaattaa
     9481 ttgcaggcac cgattgttcg ggtgactgtt agcgattcac tactcattac cacttattca
     9541 tacaaagaaa catccgaggc agttgttgga caggtataag tggcggaacc ttcatacatt
     9601 aagtgtctcc ccatcataat ggtgtcaaaa cgtcacggca acaggattaa caaactcccg
     9661 ttaagcaggc ttgaaccgac gcgcttttat catttcctga gaacagaaaa aatcaagtgc
     9721 ccctttatct gttgaacaac actgatagtt gttaaatact tccgcttatg gcacagggct
     9781 gctgttgcag ccctgacaac cttagacttc aatcgattca gaaatctcta acgcatcatc
     9841 gacttcaatg cccagataac ggactgtgct ttccagtttc ttatgaccca acagaagttg
     9901 gatcacccgg agattcttgg ttttcttgta gatcaggtaa ggttttgttc ttctcatgga
     9961 atgtgtgctg taaagcgaat cttcgagacc aagcttttct acccccccat gaaagattcg
    10021 gttgtattgc cgggttgaaa tgtgctggtt agttccgacc ctagaccgaa acaagaagtc
    10081 tttactgtgc aaattgctaa gctgtatcaa tgcagcaaca gcttctcttg tccctttggt
    10141 tatctcaaat tgaacagggc taccggtttt ctgttgcaac accgttgctc tgcttgaaac
    10201 agagccacca tatgcaacat cagatacttt gagtttgacc agatcacagc ctcgaagctt
    10261 actatccagg gccatgttga acagagctaa atcgcgagtt ttaccttcca gttcaagtcg
    10321 gattcggata ccccagatat gagatatctg aagtggtctt ttttggccta tgatacggtc
    10381 tttgttccac ggtgacgtgt tcatactcaa atctcctgca atgtgggaga tttgagtatg
    10441 gttgtccctt atgagcgagg agtggacctt gttatgctca cggccagaaa gcgctatgta
    10501 agtttggggt gagtttggtt atgctgtatt cctgccatag tcgttactcc agttaacagt
    10561 gtggtcagat gccctatagt atcacgagca ctatagggcg ttgtcttatg atccaactgc
    10621 atgatgagct taggctattg gattaaagaa ctagttagaa ctgaattaaa gacaaaggga
    10681 atcactccta aaggccaatt attcattgaa aaggaattgg tatgaacgca ggattacact
    10741 tcagtgagat cagcaaagaa caatttgata tttattttta cggtcgctca ccttacctta
    10801 agacattttc tgaggagata cgctggttta aatatgaggg gaatgggata acattgttat
    10861 ccacaataat catatgcaat attgataaag attttaacgc aatagtctta ggtcgggatt
    10921 tagataaaaa attcagagca ataaacgtct tggcttcatt tgattcaatg gatgtcttgc
    10981 ttaataattt gaacgatgac ataccaaaga tgctggcgca gcaccaaaat ggtacattca
    11041 tgcaaggcga tgaatcaacc aaaccttttt cattattctt gagtaaggtc ccagcgaaaa
    11101 aaaggaatgt ttatataaaa atgctcctcg aagaccc
//
LOCUS       DAFNBJ010000154        11050 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000154 DAFNBJ010000000
VERSION     DAFNBJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.154"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            99..812
                     /locus_tag="L2X49_003931"
     CDS             99..812
                     /locus_tag="L2X49_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBN4798939.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            844..1602
                     /locus_tag="L2X49_003932"
     CDS             844..1602
                     /locus_tag="L2X49_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798940.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1648..2997
                     /locus_tag="L2X49_003933"
     CDS             1648..2997
                     /locus_tag="L2X49_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBN4798941.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            2997..3821
                     /locus_tag="L2X49_003934"
     CDS             2997..3821
                     /locus_tag="L2X49_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798942.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            3833..4393
                     /locus_tag="L2X49_003935"
     CDS             3833..4393
                     /locus_tag="L2X49_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBN4798943.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4424..5494
                     /gene="lptF"
                     /locus_tag="L2X49_003936"
     CDS             4424..5494
                     /gene="lptF"
                     /locus_tag="L2X49_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBN4798944.1"
                     /translation="MKLVEHYIMRGTRRLVLFIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLVQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5491..6570
                     /gene="lptG"
                     /locus_tag="L2X49_003937"
     CDS             5491..6570
                     /gene="lptG"
                     /locus_tag="L2X49_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBN4798945.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6606..7778)
                     /locus_tag="L2X49_003938"
     CDS             complement(6606..7778)
                     /locus_tag="L2X49_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBN4798946.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7778..8026)
                     /locus_tag="L2X49_003939"
     CDS             complement(7778..8026)
                     /locus_tag="L2X49_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBN4798947.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8058..8972)
                     /locus_tag="L2X49_003940"
     CDS             complement(8058..8972)
                     /locus_tag="L2X49_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBN4798948.1"
                     /translation="MNQTVAVTGATGFIGKYIINNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVPGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8969..10699)
                     /locus_tag="L2X49_003941"
     CDS             complement(8969..10699)
                     /locus_tag="L2X49_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBN4798949.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHDNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIELLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
BASE COUNT         2666 a         2716 c         2873 g         2795 t
ORIGIN      
        1 gtcaatgtca caattaacca ttgtcacaat aaggttgaac ggatattctg agcacggacc
       61 acgtaatatt caatacatta tttactgccg ggtttttcgt gaatcaacgt aatatgtcaa
      121 taattaattc cacgccagtg cgtgttattg ccattgtagg atgtgatggt tcaggtaaat
      181 cgaccctcac ggcaagcctg gtaaatgaac tggcagcaag aatgccaaca gaacacattt
      241 atctcgggca atcgtccggg cgaattggcg aatggatttc acagctccct gttattggcg
      301 caccttttgg gcgttatctg cgaagtaaag cggcacatgt gcacgaaaag ccctcaacac
      361 cgcctggcaa tattactgca ctggttatct atctgctttc ctgctggcgg gcgtacaagt
      421 ttcgcaaaat gttgtgtaaa agccagcaag gctttctgct catcaccgac cgctacccgc
      481 aggttgaagt gccggggttt cgctttgatg gcccgcaatt ggcaaaaacc acgggcggta
      541 acggttggat aaaaatgtta aggcagcgcg agctgaagct gtaccaatgg atggcatctt
      601 atttgcccgt attgttgatt cgtcttggca ttgatgaaca aaccgcgttt gcgcgtaaac
      661 ctgaccacca actggcagcg ttacaggaaa aaatcgccgt tacgccgcaa ctgacattca
      721 atggcgcaaa gatccttgaa ctggatgggc gacaccccgc cgatgaaatt ctgcaagcgt
      781 cactacgcgc aattcacgcc gcgctttcct gatcaccaaa atggataggg gatgatttaa
      841 cgaatggatg cactacaaac tcaaactgtt aatagtacaa ccgcgccgca gcccaactac
      901 attccggggc tgattgcggt ggtcgggtgt gatggcaccg gtaaatccac actgaccacc
      961 gacctggtga aatcgctgca acaacactgg caaaccgagc ggcgctatct ggggctgctc
     1021 tccggcgaag acggcgacaa aatcaaacga ttgccgttgg ttggcgtctg gctggaacgg
     1081 cgactggcgg ccaaatcctc gaaaacccaa agcatgaaaa ccaaatctcc ggcgctatgg
     1141 gcggcggtga ttatgtactg cttctcgctg cgaagaatgg cgaatctacg caaggttcag
     1201 cgactggcgc aaagtggcgt tctggtggtc agcgatcgct tcccgcaggc tgaaatttcg
     1261 ggcttttatt atgacggacc ggggatcggc gtcgaacgtg cgaccgggaa aatcagcatg
     1321 tttctggcgc agcgcgaacg gcgtttatac caacaaatgg cgcaatatcg cccggaatta
     1381 attattcgcc tgggcattga tattgagact gccatctccc gcaagcctga ccatgactat
     1441 gccgagctgc aggacaaaat cggcgtcatg tcgacgattg gctataacgg cacaaaaatt
     1501 cttgagattg attccagagc gccctacagc gaagtgctgg aacaggcaca gaaggcggtt
     1561 tctctggttg ccatcgtttc tgaccgccga agtttaactt aaggctggcg ggatactttt
     1621 tactatcaac gataaggtca ggctgaattg gcgggtttta acatcaaaca ttggtttgca
     1681 gatggcgcgt ttcgcaccat tattcgcaat agcgcctggt taggctccag taatgtcgtg
     1741 agcgccttgc tggggctgtt ggcgctctcg tgtgccggta aagggatgac gcccgccatg
     1801 tttggcgtac tggtgattgt gcaatcgtac gccaagtcga tcagcgattt tattaagttt
     1861 cagacatggc agctggtggt tcagtacgga acgccagcct tgactaacaa taatccgcag
     1921 caatttcgca atgtcgtctc attttccttc tcgctggata tcgtcagcgg cgcggtggcg
     1981 attgtcggtg gcattgcctt actgccattc ctttcccatt cattaggtct ggatgaccaa
     2041 agtttttggc tggcagcgct ctattgcacg ctcattcctt caatggcttc ctccacgccg
     2101 accggcattc tgcgtgcggt agatcgcttc gatttaattg ctgtacagca ggcgacgaaa
     2161 ccttttctgc gcgcagcggg gagcgtcgta gcctggtatt ttgactttgg ttttgcgggt
     2221 tttgttattg cctggtacgt gtcgaatctg gttggcggca ccatgtactg gtggtttgcc
     2281 gcgcgcgaat tacgccgccg aaatatccat aacgccttca aattgaatct gtttgagtct
     2341 gcccgacaca ttaaaggcgc gtggagtttt gtctggtcaa ccaacattgc ccactccatc
     2401 tggtcggcgc gtaactcgtg cagcacagtg ttggtgggga tcgtgttagg acccgctgcc
     2461 gccgggttat ttaaaatcgc catgacattc ttcgacgccg ccggaacgcc agcgggtttg
     2521 ctgggtaaaa gtttttaccc agaggtgatg cgtttagatc cgcgcaccac cagaccgtgg
     2581 ttgttaggtg tgaagtccgg tttactggcg ggcggaatcg gtattctggt ggcgctcgcg
     2641 gtgtttatcg tcggcaagcc gctcatttcg ctggtgtttg gcgttaagta tctcgaagcg
     2701 tatgacctga ttcaggtgat gctgggcgca attgtgattt cgatgctggg cttccctcag
     2761 gagtcattgc tattgatggc gggaaaacag cgcgcttttc tcgtggcgca aaccatcgcc
     2821 tcaatcggct acatcgtatt gctgttcatg ttctgtcatc tgtttggtgt gctgggagca
     2881 gcgtttgctt acttcggtgg gcaatgtctg gacgtggcgc tctcgttaat tccaacctta
     2941 aaggcatttt ttcagcgcca ttccttgctt tataacgcag ccggagagaa atcctgatga
     3001 tgagaaaata tttcccgctt gaggcgagcg agcgtctatt tgttgcgatt gaagaagatg
     3061 atgtcgttga tgcacaggtt agtttgccgc cgactattgc actaagttgt accaccgaaa
     3121 tcattcatga taattacgcg ttatgcctgc aattctggct caatggcgtt gatcgtcagg
     3181 aattactgcg tttagttcgc aaacaagcca aaggcgatga gctgactgct gatgagcgca
     3241 aacaatttaa atacatgcga gcgcgctaca agcacctgcg ttttgcgcaa cggctgtatt
     3301 taaagaagca tcaggccgga tttttattcg gcaaaacgac cgtttttctt gggcgttttc
     3361 aggacggctt tcgtaacggt aagaaaaata ttgtgtcgta ttacggcaac ctgttacgga
     3421 tttatttaag ctctccggtc tggtcgttgg tgaactattc attacgccat agccagttag
     3481 aaagcgtaag cagttttatt gcctatcggc aaaagcaaat gcataccctg aaagagatta
     3541 ttgccaaacc gcgactaacc ggcagggaat ttcatgatgt gcgaaaaatc atcagccaac
     3601 aggtttctta ttacgacacc ttaagatcgc ttgatcctga aaataaagag gcgttacaga
     3661 tttcccgttt cctcgccgcc attaatggcc tgatgggaga taaacacgac gacatggtgg
     3721 cggatgatat ggaaaaccgc cagtcgtatg atgcaccgtt ggcgctggat agcgatattc
     3781 gtcagcgctt agagctgctg atttcgcgtt ttccgctata accggagcaa tgatgtcatc
     3841 tcgtttgatt attgcgctga ttatcatgct tttagccccg ggtgtgcagg cacacaattt
     3901 cgtcactggc aaaacggtta cccctgttta tattcaggaa ggcggcgagc tgcttctgaa
     3961 tagcgacgac gaaattcact accaaaagtg gaacagcaca caattggcgg gaaaagtccg
     4021 cattatccag tatatcgccg ggcgtaagtc ggcgaagaaa aagaactcac tgttaattaa
     4081 agcggttgaa gcggcaaact ttccgcagga tcgttttcag cccaccacca ttgtgaacac
     4141 cgatgatgcc atctttggta ctggctattt tgttgttggc aaaatcgaaa aaaataaacg
     4201 ccgctatccg tgggcgcaat tcgttattga cggcaatgga cagggacgcg ttgcctggcg
     4261 cttgccggag cagagttcaa ccattctggt gctgaataaa gccgggcaaa ttcagtgggc
     4321 aaaggacggc agcctgacgc cggaagaggt agatcatgtc attgcgctgg cgcagaaact
     4381 gatcaatgaa taaaaaattc aaaggattga gggcgttagt gagatgaagc tggttgaaca
     4441 ctacatcatg cgtggtacgc gccgtctggt gctgtttatc gtcggttttc tgatctttat
     4501 tttcgccagc tactccgcac agcgttatct gaccgaagcg gcaaatggga cattagctct
     4561 tgatgttgtg ctggatatcg ttttctacaa agtgctgatt gcactggaga tgttgttacc
     4621 tgttggactg tatgtgtcag ttggcgtgac gctagggcag atgtataccg attcggaaat
     4681 taccgctatc tctgcggcgg ggggcagtcc gggacgcttg tacaaagccg ttctttatct
     4741 ggcgataccg ctaagtattt ttgtcaccct tctgtcgatg tatggtcgtc cgtgggctta
     4801 tgcgcagatt tatcaactgg agcagcagtc gcagtcggag ctggatgttc gccagttgcg
     4861 ggcaaagaaa tttaacacta acgataacgg acgaatgatc ctttcgcaga cggttgatca
     4921 ggataataat cgcctgactg acgcgctgat ttatacttct actgccaatc gaacccgcat
     4981 tttccgcgcc cgttcggttg atgtggttga cccatcaccc gagaaaccga ccgttatgtt
     5041 gcacaacggc actgcctatc ttctcgatca tcaggggcgt gacgacaacg aacagatcta
     5101 ccgtaatctg caattacatc tgaatccgct ggttcaaagc cctaacgtca aacgcaaagc
     5161 gaaatcggtc acggagctgg cgcgctccgt ctttcctgcc gatcatgccg aactgcaatg
     5221 gcgacaaagc cgtggtctga cagcattgtt gatggcgctg ctggccattt cattaagtcg
     5281 ggtaaaaccg cggcaagggc gattttcaac gttattgcca ctgacgttgc tgtttattgc
     5341 cattttttat ggcggcgacg tctgccgtac gctggtggct aacggcgcga ttcccctcat
     5401 tcctggtttg tggttagtgc ccggactcat gctaatgggc ctgctgatgc tggtcgcacg
     5461 cgacttctct ttgctgcaga aattttcccg atgaatgttt tcagtcgcta tttaatccgt
     5521 catctctttc tcggttttgc cgccgccgca gggctattgc tgccgctttt taccaccttc
     5581 aacttgatta acgaactgga tgatgtcagc ccgggcggtt atcgctggac tcaggcggtg
     5641 ctggtggtgc taatgacctt accgcgcaca ctggtcgaac tttcgccgtt tatcgcgtta
     5701 ttgggtggta ttgttggact ggggcagtta tcgaaaaaca gtgagcttac cgccattcgc
     5761 agcacggggt tttctatctt ccgtattgca ctggtggcgc tggttgccgg gatattgtgg
     5821 actgtttcgt taggcgcgat agatgagtgg gtggcgtcgc cattgcagca acaggcgttg
     5881 caaatcaaat cgaccgccac cgcgttgggg gaggacgatg acattaccgg caatatgctg
     5941 tgggccaggc gtggtaatga atttgtgacg gtgaaatcgc tgaacgagca gggccagccg
     6001 gtgggcgtgg agatatttca ttatcgcgac gatctctcgc ttgaatccta catttatgca
     6061 cgcagtgcca ccattaaaga cgacaaaacg tgggtcctgc atggtgtgaa tcataaaaaa
     6121 tggctgaatg gcaaagaaac gctggaaaca ttagataatc ttgcctggca atcggccttc
     6181 accagtatga atcttgaaga gttatcaatg ccggggaata ctttttctgt ccgtcagctt
     6241 aatcattaca tccattattt gcaggaaacc gggcaaccca gcagcgaata ccgccttgca
     6301 ctgtgggaaa aactggggca acccatcctg accctggcga tgattttgct ggctgtgccg
     6361 tttaccttta ctgccccgcg ttcgcctggg atgggtagcc gtctcgctgt aggcgtcatc
     6421 gtgggcttac tcacctggat cagctatcaa atcatggtta atctgggatt gctatttgcg
     6481 ttaagcgcac ctgttaccgc gctcggttta ccggtagcgt ttgtgttggt ggcgttgagc
     6541 ctggtgtatt ggtatgacag acaacattaa acccgccgtg gcgcaaacac cacggcgggc
     6601 tcacattact gcttcagagt agcaaatgcc tgaatgatgg catcgatttg ctcacgactg
     6661 tgggcagcat ttacgctaca gcgcagcagc gtgatgcctg caggcgcggc aggcggcagg
     6721 ataagattca catacacgcc cagcgagata agctcgcgcc agatgcgtaa accttcctct
     6781 ttcgaaccga ttattactgg cactaccggg ctaatatgtg aacttaactc ataacccagt
     6841 ttttgcagac cgtcatacag atggttggcg ttatccatca gcttttgccg cagctctggt
     6901 tgtgtggcaa tagttcttaa cgacgagcgc acggtcgcaa tgcaggacgg agaaggagag
     6961 gcggtaaaaa tatacgggcg gctgccgtag cgcagcactt ccatcgcttc cgaaccgaca
     7021 gcaaaaccgc caatcgaggc cagactttta ctgaaagtac caacgataat atccacatca
     7081 tcttcaacgc cgacagcttc agccagtccg cgccccgttg cgcctaacac gccaaacgaa
     7141 tgcgcttcat cgacaatcaa ataaccaccg agacgacgtt tgatatcaac aatttccgcc
     7201 agcggcgcaa cgtcaccgag catgctgtag atgccctcaa cgatgataat cgcctctttc
     7261 gcccgttcac cgaggcgaac catgcgccgt tccagatctt tagcatcgtt atggcggaaa
     7321 cgaataattt ccgcgccacc gagagaacag gcgtcataaa tactggcgtg gctgtcggca
     7381 tccagtaaca ccacggcgtt atggtcggca agtgcgctga tgacgcctaa attagcggta
     7441 taaccggttg aaaaaacgat agcggtaggg cgattgaaaa actcggcaat ctctttctct
     7501 aacgccaggt gcggcgcata actgccgttt gccatacgag aaccggtggt gccggtgcct
     7561 tgggccgcta acgctgcctg accttcgctg atggcatcat gattaaaggt taaaccgaga
     7621 taattattgg tcccggccag aatcactttt ttattgccga tccggccttc ggtggcggaa
     7681 tacacttcat caatacaggt gccaaacggt gttaacccgt tgtcactaaa ttgcagtcgc
     7741 tcgccagcaa ggcgcgcata tttatcgtat agccccatta tttattctcc agccatggaa
     7801 gtagagtttc cattaattgc gcaggggttt tgacatccag cagaatatta ataggaatgg
     7861 aaatatcgaa tctgtcttcc agcatcatta agagatccat cactttgatc gactccaggc
     7921 caagatcgtt gacgagatcg ctgtcgggct taatttccac accgttttcg accaaatcct
     7981 gtaaacagga aaggatgtaa tccattacta tttcacgatt taccatagaa acgttaacac
     8041 acctttaaac aagacaatca aaatagcccg ttgcgtaatg cgtgttccag gctaatcccg
     8101 ggaaaccaat taatatcttc agaaatacgg ttattacttg ccgaccagtc ggcgtgggtt
     8161 aattcacgaa ttttcgagcg ggtcagcata ggttctttac ctgccaggcg actcaacgcg
     8221 gtactgatat ccgcaaggca ggtgagtacc ggcagaggaa tgccaaccat tcgcacggaa
     8281 ccacaacggg cgtcggcggc aagctgtcgt acgtgttgcc agtcatagcc gccggggacg
     8341 ccatcgcata attcataggt ttgcgtctgt acagtttcgg cgcttaacca ctgacccact
     8401 gcttgcgcga aatcggtgac gtgcaggaaa gagagctgtg tatctggcgc accaagtcgt
     8461 ggcagcaggc cgcgcagcat ccagtcaaac agcggtttta actctttatc gccgggacca
     8521 tacacggctg tcgggcgaaa aacgcccagc gtaatttcgt cagccattgc agtcagacgt
     8581 tgttcggcga cgtgtttgga atttgcgtac caggagagct cgggatggcg cgccgccagc
     8641 gaagagataa acagaaaacg ttggcaaaag ccgctctctt ttgctgcttg catcaggcgc
     8701 aggctgccgt caacgttaca gtgggtaaaa atctcttctt tgtgcccgcg cacttgcccg
     8761 gcgcaatgga ccaccgcgct ggctccggca accagcttgc taagtgaatg agtatcttcc
     8821 agcgaaccgc gcacccaggt gagattatca ttgacgtgag cgcgggcagt acgcgtcaat
     8881 gcgcgaacgt gaaagccgcg ggcgagcagg ttattgataa tatatttacc gataaacccg
     8941 gtagcgcccg tcaccgcgac agtttgattc atgccaggga ttcctgcaca tgaagactgg
     9001 cagcataagc cttctgataa cgttttttcg cttccgcacg ggcaggcttg ccggaggacg
     9061 ttcggggaat actgtggggc ggcaacagct cgatagccgc ggtcacgcca aattcgcttt
     9121 ggatccgtgc cgccagcgcg tggataagct gcccgcgacg ttcttcgtcg ctgatccgac
     9181 actggatctg caaaatgatt ttttcctggg cggtaacaaa agcaatcgca tcgccagaat
     9241 gaatttccgg ttcttgttcc gcaatatatt caatatcctg cggccagata ttacggccac
     9301 gaataataat cagatcttta atgcgtccgg tgacatacag ataaccgtcc agcagataac
     9361 cgaggtcgcc ggtgtctaac cagcccgtcg cggcaatctc gtcttgcgaa gcctggtcgc
     9421 caaagtaacc gctcatcaga ctgggaccgg agatgcaaat atggcctacc acacgttccg
     9481 cgaccggcat acctgcttca ttgcggattt caataccatg ttccggcaac gctttgccgc
     9541 agttgacgaa agtcgatacg gcgcgtgtct ctgcacccgg cgcgacggct ttaccctgat
     9601 attcgaggat gtcgcgatcc acttcgttaa ccacaacccc ggaggcttca tcagagaagc
     9661 tgacagccag cgcattttct gccagtccgt agcacggcat gaaagtttta ttgtcaaagt
     9721 taacctgacg gaaacattca gcgaattgat ggagttgttc tgcggagatg ggttctgcac
     9781 caataccagc gacgcgccag caggaaagat ccagttcagc gagatctttt tcattcacgc
     9841 ggcgctggca caattcatag ccaaacggcg gcgcaacgga aacggtgccg cgatttttac
     9901 tgatcaattt aagccattgc agaggacgca tggcaaaatc ctgagtgcgc aaataatcta
     9961 ctgaaagctg cgtggcgacg ggggtcagga gaaagccgac cagtcccata tcatggtaga
    10021 aaggcagcca ggagacgcag cggtcgccag ggcgtaattt aatgccgtcg tggcttatag
    10081 cacgtagatt agccatcact tcgcgatggg tgataatgac gccacgggga aaacgggtgc
    10141 tgccggaggt gtactggagg taggcgatat cgttcggaac tggacgctgg agcgcaacat
    10201 cggcttccgg taatgcctta aaccaggcgt ggcttaaaac atgtaattcg gggttgtcat
    10261 gcgtcgcggc attgaccagt ggcaaccact catcaccagt gataatggct gcgggctggc
    10321 aacttgccag taaaccctgc aatttggcgc tccaggaatc ccgctgacca acgcccattg
    10381 gaatcgccaa cgggacggcg actaagccgg catactggca ggcaaaaaaa gcctctacga
    10441 acccgctact tgtttcggca atcagtgcca cgcgatcgcc ttttttcagg ttcagcgata
    10501 acaaccgctt cgcaccagct tcggcacgcg tttttaatgt ctgatattcc agttgatctt
    10561 caagttggca acgtctgtca taaaaattca ttccggcgct actcagagcg gcgtagtcca
    10621 aagcatcaac cagcgttgga aaatcggcat agcgcatagg tagggaatgc gtaaagattt
    10681 tattagacat atacaccacg aaaaaattgg atatcaaaaa taaaatgaca ataatgcggt
    10741 ggattatata ggcataaaag ataaatgaga tagtattttc atcggtgtga aaatgaacat
    10801 tgatttaaat aatgagttta agttttttgt ttttattgtg aatgatattt aatttatgaa
    10861 tatattaaga agaagccttg tccggtcgcc ttaaatcgac aaaaggcatt gaggcgcttc
    10921 tgtgtagtct ctaaccttat cataatcatt aataaatact atttattctt tgtttgtatt
    10981 cattgggttg attattaagg tgtcgttcgt tgctgttgca acctgttttt caccgttaat
    11041 tatttgttat
//
LOCUS       DAFNBJ010000155        10895 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000155 DAFNBJ010000000
VERSION     DAFNBJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.155"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            158..1372
                     /locus_tag="L2X49_003942"
     CDS             158..1372
                     /locus_tag="L2X49_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBN4798950.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1439..2722
                     /locus_tag="L2X49_003943"
     CDS             1439..2722
                     /locus_tag="L2X49_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4798951.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            2974..3891
                     /gene="oxyR"
                     /locus_tag="L2X49_003944"
     CDS             2974..3891
                     /gene="oxyR"
                     /locus_tag="L2X49_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBN4798952.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3874..5274)
                     /gene="sthA"
                     /locus_tag="L2X49_003945"
     CDS             complement(3874..5274)
                     /gene="sthA"
                     /locus_tag="L2X49_003945"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBN4798953.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5605..6255
                     /gene="fabR"
                     /locus_tag="L2X49_003946"
     CDS             5605..6255
                     /gene="fabR"
                     /locus_tag="L2X49_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBN4798954.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6255..6614
                     /locus_tag="L2X49_003947"
     CDS             6255..6614
                     /locus_tag="L2X49_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBN4798955.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6654..7754)
                     /gene="trmA"
                     /locus_tag="L2X49_003948"
     CDS             complement(6654..7754)
                     /gene="trmA"
                     /locus_tag="L2X49_003948"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBN4798956.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7868..8058
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8123..9967
                     /gene="btuB"
                     /locus_tag="L2X49_003949"
     CDS             8123..9967
                     /gene="btuB"
                     /locus_tag="L2X49_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBN4798957.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9912..10769
                     /gene="murI"
                     /locus_tag="L2X49_003950"
     CDS             9912..10769
                     /gene="murI"
                     /locus_tag="L2X49_003950"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBN4798958.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2502 a         2584 c         2916 g         2893 t
ORIGIN      
        1 taattattca ggtggttttt gagggtattt tttctcctcg gcggaaattt ttatttttaa
       61 tgtgatcacg gttgaataat cgctctttct ctcttgtatg gtagtaggcg taaaattaag
      121 tttgaagcgt ctatttacaa cacaggagat tgcaataatg aaaattattg ctgcagatgt
      181 ttttgttact tgcccagggc gtaatttcgt tacgcttaaa ataaccactg aaagcggact
      241 ttgtggatta ggagatgcca cgttaaatgg tcgcgaattg tcggtcgctt cttatctgaa
      301 agatcatctt tgtccacaac taattggccg cgatgccagc cgtatcgaag atatctggca
      361 attcttttat aaaggtgctt actggcgacg tggccccgtc acgatgtcag ccatttctgc
      421 cattgatatg gcgttgtggg atatcaaagc taaagcggca aatatgccat tgtatcaact
      481 gcttggtggt gcttctcgtg aaggcgtgat ggtttattgc cacaccaccg ggcggacgat
      541 tgatgaagtc cttgaagact atgcaaagca ccagcagatg ggctttaagg cgattcgtgt
      601 gcagtgcggt gtaccgggta tgcaaactac ttatggcctg gcgaagggca aaggcctggc
      661 gtatgaaccg gcaaccaaag gcctgtggcc agaagagcag ttgtggtctt cagaaaagta
      721 tctcgatttt acgccgaagt tatttgaagc ggtgcgtaat aagttcggtt ttaacgaaca
      781 tctgttgcat gacatgcacc atcgcctgac ccccatcgaa gcggcgcgct ttgggaagag
      841 cattgaggat taccgcctgt tctggatgga agatccgacg cctgcagaga accaggaatg
      901 ttttcgtctg attcgccagc ataccgtgac gccgattgcg gtcggtgaag tgtttaacag
      961 tatctgggac tgcaagcagt taatcgaaga gcagctgatt gactatattc gcacgacgat
     1021 cacccatgca ggcggcatta ccgggatgcg gcggattgcc gattttgcat cgctttatca
     1081 ggtacgtacc ggttcgcatg gcccttcaga tttatcgcct gtttgtcatg cggcggcgct
     1141 gcattttgac ctatgggtac caaattttgg cgttcaggag tacatgggct attccgaaca
     1201 aatgcttgaa gttttcccgc acagctggcg cttcgaggaa ggttatatgc acccaggcga
     1261 tgaaccgggg ctgggtattt ctttcgatga gaaactggcg gcgaaatatc cgtatgaccc
     1321 ggcgtatctc cccgtcgcac gactggaaga tggcacattg tggaactggt aataacggtt
     1381 cgcttaatta ttttctgaat agtcatcaaa tcgattaatt ccgctcaagg agctaaaaat
     1441 ggacgttgat gtgtcaacca gtgttgcggg taataaaccg caacggattc gtcgtattca
     1501 gacagtgacc ctggttttat tatttatggc ggggatcgtt aattttctcg accgttcgtc
     1561 attgagcgtg gcaggtgaag cgattcgtgg cgagttagga ttatcggcga cagagtttgg
     1621 tgttttgctt tctgcatttt ctctgtctta tggtttttca caactacctt ccggtatttt
     1681 gctagaccgc tttggcccac gaattgtttt aggcgcaggc ttaatattct ggtcattaat
     1741 gcaggcatta acaggaatgg ttaattcttt tagccacttt attttaatgc gtatcggtct
     1801 gggtattggc gaagcgccat ttatgcctgc gggagtaaag tcgatcaccg actggtatgc
     1861 acaaaaagaa cgcggcacgg cgctggggat ttttaactca tctaccgtta tcggtcaggc
     1921 tatcgcgcct cctgctcttg tattgatgca gctggcatgg ggctggcgga cgatgttcgt
     1981 tatcatcggc gtggcaggga ttctggttgg gatctgttgg tacgcgtggt atcgcaaccg
     2041 ggcgcagttt gtcctgactg acgaagaacg gacgtatctc tccgcgccgg ttaaaccgcg
     2101 tccacagctg caatttagcg agtggctggc gctgtttaag catcggacaa cctgggggat
     2161 gattttaggt ttctctggcg tcaattatac cggttggctc tacatcgcgt ggttacccgg
     2221 ttatttgcaa gccgagcaag gtttcagtct ggcgaaaacc ggttgggtgg cggcgattcc
     2281 tttcctcgcg gcggcagtcg ggatgtgggt taacggtatt gttgtcgatc gactggcgaa
     2341 aaaaggctac gacctggcga agacgcgtaa aacggctatt gtctgcggtt tgatgatgtc
     2401 ggcattaggc acgttgctgg tcgtgcaatc ttcctcgcca gcccaggcgg tagcgtttat
     2461 ctcaatggcg ctattctgcg tgcatttcgc tggaacgtct gcatgggggc tggtgcaggt
     2521 gatggtgtca gaaacaaaag tggcttccat cgccgggatt caaaactttg gcagttttgt
     2581 ttttgcttcc tttgctccga tcgtaaccgg ttgggtagtg gataccacac actcgtttaa
     2641 tctggcgttg gttatcgcgg cctgtgtgac gttcaccggg gcgctgtgtt acttctttat
     2701 tgtcaaagat cgcattgagt aatcgttact atcaggctct cttgctgtgg gcctgtagaa
     2761 taaaaaaaag cggatcctgg agatccgcaa aagttcacgt tggctttagt tattcgagtt
     2821 gagaaactct cgaaacgggc agtgacttca agggttaaaa gaggtgccgc tccgtttctg
     2881 tgagcaatta tcagtcagaa tgcttgatag ggataatcgt tcattgctat tctacctatc
     2941 gccatgaact atcgtggcga tggaggatgg ataatgaata ttcgtgatct tgagtacctg
     3001 gtggcattgg ccgagcaccg ccattttcgg cgtgcggcag attcctgcca cgttagccag
     3061 ccgacgctta gcgggcaaat tcgtaagctg gaagatgagt tgggcgtgat gttgctggag
     3121 cggaccagcc gtaaagtgtt gttcacccag gcgggaatgc tgctggtaga tcaggcgcgt
     3181 accgtactgc gtgaggtgaa agtccttaaa gagatggcaa gccagcaggg cgagacgatg
     3241 tccgggccgc tgcacattgg tttgattccc acagttggac cgtacctgct accgcatatt
     3301 atccctatgc tgcaccagac ctttccaaag ctggaaatgt atctgcatga agcacagacc
     3361 caccagttac tggcgcaact ggacagcggc aaactcgatt gcgtgatcct cgcgctggtg
     3421 aaagagagcg aagcattcat tgaagtgccg ttgtttgatg agccaatgtt gctggctatc
     3481 tatgaagatc acccgtgggc gaaccgcgaa tgcgtaccga tggccgatct ggcaggggaa
     3541 aaactgctga tgctggaaga tggtcactgt ttgcgcgatc aggcaatggg tttctgtttt
     3601 gaagccgggg cggatgaaga tacacacttc cgcgcgacca gcctggagac gctgcgcaac
     3661 atggtggcgg caggtagcgg gatcacttta ctgccagcgc tggctgtgcc gccggagcgc
     3721 aaacgcgatg gggttgttta tctgccgtgc attaagccgg aaccacgccg cactattggc
     3781 ctggtttatc gtcctggctc accgctgcgc agccgctatg agcagctggc agaggccatc
     3841 cgagcaagaa tggatggcca tttcgataaa gtgttaaaac aggcggttta aaccgtttaa
     3901 cgcagctacc cgataggctt ccgccatggt cgggtagtta aaggtggtgt tgacgaagta
     3961 ctcaatagtg ttgccgccac ctttctgttc cataatcgcc tgaccgatat gaataatttc
     4021 ggcagcgcgc tcgccaaagc agtgaatacc cagaatctct tttgtttccc gatggaacaa
     4081 aattttcagc gtgcccacgt tcatgccgac gatttgtgcg cgtgccagat gtttaaactg
     4141 ggcgcggccc acttcatatg gcactttcat cgcggtcagc tgctgttcgg ttttgcccac
     4201 agagctgatt tccgggatgg tgtaaatgcc ggtagggata tcttcaatca gatgcgcggt
     4261 ggcttcgcct tttaccagcg cctgcgcggc aatgcgcccc tggtcatagg ccgctgatgc
     4321 caggctcgga taaccaatca cgtcgcccac cgcgtaaacg tgcggctgtg cggtctgata
     4381 catgctgttg accttcagct gtccgcgact gtcagtttcc agcccaatgt tctgtaacgc
     4441 cagcgaatcg gtattaccgg tacgaccgtt ggcatagagc aggcagtcag ctttcagttt
     4501 tttacccgac ttcagatgca tgatcacacc gtcgtcacag ccttcgatct tctcgtactc
     4561 ttcgttgtga cgaatcacta cgccactgtt ccagaagtga taggagagag aatctgacat
     4621 ctcttgatcg agaaatgcca gcaggcgatc gcgggtgttg atcagatcca cttttacatc
     4681 cataccgcgg aagatcgacg catattcaca gccgatcact ccagcaccat agataagtac
     4741 atggcgcggt tcgtggtgca tgctaagaat tgagtcgctg tcgtaaatgc gtggatgggt
     4801 gaaatcaaca tctgttggat gatatggacg agagccgcag gcaataacaa atttttcagc
     4861 ggttagtgtt tcaacgctgc cgtccgggca atccagcgcc aacgtatgct cgtcaacaaa
     4921 gcgagcgttt ccctgcaata tttcacagtg attacgttcg taaaatccct gacgcatgcg
     4981 cgtttgttga ttaatcacgt tatcggcatg gttaaggata tcggcaaaag aagagcggag
     5041 cagtcgggaa tggtcgctgt aaagtgggtt ttgattgaat tctataatgc ggctgacggc
     5101 gtgacggaga gctttcgacg ggatggtgcc ccagtgggtg caaccgccgc caacattttg
     5161 ataacgctcg ataactgcga cgcgcgcacc ttgcttaacc aggcccattg cagcgccttc
     5221 gccgccgggg ccggaaccta ttactatggc atcgtaatcg taggaatgtg gcatggtagg
     5281 gcttacctgt tcttatacat aaaagcaaca gaatggtaac attttatcgc gggtaagcca
     5341 attgatcccc gtcatttatc tggctatatc ctgagcggcc tttgctttgt ctgtttctta
     5401 cttttgccct gacgttttat tggattttta tcgacgatac tctccgttta agcggcaggt
     5461 ttccgctgta cgtaaaagaa ccggccaaag aattgcagta aatatgtttt attgcgttac
     5521 cgttcattca caatactgga gcaatccagt atgttcattc tctggtatag tgccagcagt
     5581 acttttggca aggattcaga catcgtgatg ggcgtaagag cgcaacaaaa agaaaaaacc
     5641 cgccgttcgc tggtggaagc cgcatttagc caattaagtg ctgaacgcag cttcgccagc
     5701 ctgagtttgc gtgaagtggc gcgtgaagcg ggcattgctc ccacctcttt ttatcggcat
     5761 ttccgcgacg tagacgaact gggtctgacc atggttgatg agagcggttt aatgctacgc
     5821 caactcatgc gccaggcgcg tcagcgtatc gccaaaggcg ggagtgtgat ccgcacctcg
     5881 gtctccacat ttatggagtt catcggtaat aatcctaacg ccttccggtt attattgcgg
     5941 gaacgctccg gcacctccgc tgcgtttcgt gccgccgttg cgcgtgaaat tcagcacttc
     6001 attgcggaac ttgcggacta tctggaactc gaaaaccata tgccgcgtgc gtttactgaa
     6061 gcgcaagccg aagcaatggt gacaattgtc ttcagtgcgg gtgccgaggc gttggacgtc
     6121 ggcgtcgaac aacgtcggca attagaagag cgactggtac tgcaactgcg aatgatttcg
     6181 aaaggggctt attactggta tcgccgtgaa caagagaaaa ccgcaattat tccgggaaat
     6241 gtgaaggacg agtaatgaaa caagcaaatc aagatagagg tacgctgctg ctggcgttgg
     6301 ttgctggctt atcgattaat ggtactttcg cagcgctgtt tagctccatt gtgccatttt
     6361 ctgtattccc gattatttcc ctggtgctga cggtttactg cctgcatcaa cgttatctta
     6421 atcgcaccat gccggtaggc ttgccgggtc tggcagctgc ctgttttatt ctcggcgtac
     6481 tgctgtacag cacggtagtt cgtgcggaat atccggatat cggctctaac ttcttcccgg
     6541 cagtactctc cgtcattatg gtgttctgga ttggcgcgaa gatgcgtaac cgtaagcagg
     6601 aagttgctga gtaatcggta ttatgccgga tgctggcgca tccggcatgg gttttacttc
     6661 gcggtcagta atacgccgca ctccatatgg tgcgtgtagg ggaactgatc aaacagagcc
     6721 agacgttcga ccttgtgcgt ctggcttaat gtttccagat tcttgcataa cgtttccgga
     6781 ttgcaggaga tatacagaat acgtggatac gcctgcacca ttttctcggt ttcactgtcc
     6841 agaccgctgc gcggtgggtc gacaaaaatg gtttcgcact gataactctt caagtctatc
     6901 ccttgcaggc gattaaactc gcgtacacca ttcatcgcct gagtaaattc ttctgccgcc
     6961 atacgaataa tttgtacgtt atcaatatgg ttagctgcga tgttgtattg cgcagcagca
     7021 accgacggct tagcgatttc ggtggctaat acccgatcaa aattgcgcgc cagtgctaat
     7081 gaaaagttac cgttgccgca gtacagctcc agtaaatcgc cttttgagcc tttggttacg
     7141 tccagcgccc attccagcat ctgaatattc atcgccgcgt tcggctgggt aaaactgttt
     7201 tctacctgac ggtagatcat ctctttccct gcgaccggca gacgttcatc gatgtaatct
     7261 tgatccagct cgattttggt tttcgttgcc cgaccaatca gatgcacatt cagattctgc
     7321 gcgcgcagtg catcgcgcag ggcttccgct tcctgacgcc actcatcatc cagcttttta
     7381 tggtacaaca gggaaaccac cgcctgatta ctcagcgtgg tgaggtaatc aatctggaac
     7441 aacttgtggc gcagaacggg attattacgc acacccgcaa tcatcgccgt catcaactgg
     7501 ttgatgagtt cactggcagc ggggaagcta tccacgcgga tgcggctttt ggtttgttga
     7561 tcgaaaatga tgtgatacag gtcatcgcca tcgtgccaga tgcggaactc cgcacgcatc
     7621 cggtaatgac tgaccggcga gcgaaacact tccggaacca ggtcagaaaa cggtgccatc
     7681 atactttgca aacgtaccac tttttcggct aactgcgctt catactgttc tgttggaagg
     7741 tgttcggggg tcatgatgta tcctgaaaaa ttaaagtacg cggcgattgt agggattgct
     7801 catcagatgt ccagatcttg atgaattcct atttgtgagc tacgtctgga cagtaacttg
     7861 ttacaacctg tagcatccac ttgccggtcc tgtgagttaa tagggaatcc agtgcgaatc
     7921 tggagctgac gcgcagcggt aaggaaaggt gcgatgattg cgttatgcgg acactgccat
     7981 ccggtgggaa gtcatcatct cttagtatct tagatacccc tccaagcccg aagacctgcc
     8041 ggccaacgtc gcatctggtt ctcatcatcg cgtaatattg atgaaacctg cggcatcctt
     8101 cttctattgt ggatgcttta caatgattaa aaaagcttcg ctgctgacgg cgtgttctgt
     8161 cacagccttt tccgcttggg cacaggatac cagcccggat actctcgtcg ttactgctaa
     8221 ccgttttgaa cagccgcgca gcactgtgct tgcaccaacc accgttgtga cccgtcagga
     8281 tatcgaccgc tggcagtcga cctcggtcaa tgatgtgctg cgccgtcttc cgggcgtcga
     8341 tatcacccaa aacggcggtt caggtcagct ctcatctatt tttattcgcg gtacaaatgc
     8401 cagtcatgtg ttggtgttaa ttgatggcgt acgcctgaat ctggcggggg tgagtggttc
     8461 tgccgacctt agccagttcc ctattgcgct tgtccagcgt gttgaatata tccgtgggcc
     8521 gcgctccgct gtttatggtt ccgatgcaat aggcggggtg gtgaatatca tcacgacgcg
     8581 cgatgaaccc ggaacggaaa tttcagcagg gtggggaagc aatagttatc agaactatga
     8641 tgtctctacg cagcaacaac tgggggataa gacacgggta acgctgttgg gcgattatgc
     8701 ccatactcat ggttatgatg ttgttgccta tggtaatacc ggaacgcaag cgcagacaga
     8761 taacgatggt tttttaagta aaacgcttta tggcgcgctg gagcataact ttactgatgc
     8821 ctggagcggc tttgtgcgcg gctatggcta tgataaccgt accaattatg acgcgtatta
     8881 ttctcccggt tcaccgttgc tcgatacccg taaactctat agccaaagtt gggacgccgg
     8941 gctgcgctat aacggcgaac tgattaaatc acaactcatt accagctata gccatagcaa
     9001 agattacaac tacgatcccc attttggtcg ttatgattcg tcggcgacgc tcgatgagat
     9061 gaagcaatac accgtccagt gggcaaacaa tgtcatcgtt ggtcacggta gtattggtgc
     9121 gggtgtcgac tggcagaaac agactacgac gccgggtaca ggttatgttg agaatggata
     9181 tgatcaacgt aataccggca tctatctgac cgggctgcaa caagtcggcg attttacctt
     9241 tgaaggcgca gcacgcagtg acgataactc acagtttggt cgtcatggaa cctggcaaac
     9301 cagcgccggt tgggaattca tcgaaggtta tcgcttcatt gcttcctacg ggacatctta
     9361 taaggcacca aatctggggc aactgtatgg cttctacgga aatccgaatc tggacccgga
     9421 gaaaagcaaa cagtgggaag gcgcgtttga aggcttaacc gctggggtga actggcgtat
     9481 ttccggatat cgtaacgatg tcagtgactt gatcgattat gatgatcaca ccctgaaata
     9541 ttacaacgaa gggaaagcgc ggattaaggg cgtcgaggcg accgccaatt ttgataccgg
     9601 accactgacg catactgtga gttatgatta tgtcgatgcg cgcaatgcga ttaccgacac
     9661 gccgttgtta cgccgtgcta aacagcaggt gaaataccag ctcgactggc agttgtatga
     9721 cttcgactgg ggtattactt atcagtattt aggcactcgc tatgataagg attactcatc
     9781 ttatccttat caaaccgtta aaatgggcgg tgtgagcttg tgggatcttg cggttgcgta
     9841 tccggtcacc tctcacctga cagttcgtgg taaaatagcc aacctgttcg acaaagatta
     9901 tgagacagtc tatggctacc aaactgcagg acgggaatac accttgtctg gcagctacac
     9961 cttctgaacc acgtcccacc gtgctggtgt ttgactccgg cgtcggtggg ttgtcggtct
    10021 atgacgagat ccggcatctc ttaccggatc tccattacat ttatgctttc gataacgtcg
    10081 cttttccgta tggcgaaaaa agcgaagagt ttattgttga gcgagtggtg gcaattgtca
    10141 ccgcagtgca agaacgttat ccccttgcgc tggctgtggt cgcttgcaac accgccagta
    10201 ccgtttcact tcctgcatta cgcgaaaagt tcgacttccc ggttgttggt gtcgtgccgg
    10261 cgattaaacc tgctgcacgt ctgacggcaa atggcattgt cggattactg gcaacccgcg
    10321 gaactgttaa acgttcttat actcatgagc tgatcgcgcg tttcgctaat gaatgccaga
    10381 tagaaatgct gggctcggca gagatggttg agttggctga agcgaagcta catggcgaag
    10441 atgtttctct ggatgcacta aaacgtatcc tacgcccgtg gttaagaatg aaagagccgc
    10501 cagataccgt tgtattgggt tgcacccatt tccctctact acaagaagaa ctcttacagg
    10561 tgctgccaga gggaacccgg ctggtggatt ctggcgcagc gattgctcgc agaacggcct
    10621 ggctgttaga acatgaagct ccggatgcaa aatctgccga tgcgaatatt gccttttgta
    10681 tggcaatgac gccagaagct gaacaattat tgcccgtttt acagcgttac ggcttcgaaa
    10741 cgctcgaaaa actggcagtt ttaggctgat ttggttgaat gttgcgcggt cagaaaatta
    10801 ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc actgacacgg
    10861 aacaacggca aacacgccgc cgggtcagcg gggtt
//
LOCUS       DAFNBJ010000156        10885 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000156 DAFNBJ010000000
VERSION     DAFNBJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.156"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(44..128)
                     /locus_tag="L2X49_003951"
     tRNA            complement(44..128)
                     /locus_tag="L2X49_003951"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:Tyr,seq:gta)
     gene            complement(288..1130)
                     /gene="purU"
                     /locus_tag="L2X49_003952"
     CDS             complement(288..1130)
                     /gene="purU"
                     /locus_tag="L2X49_003952"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBN4798959.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1180..1638)
                     /locus_tag="L2X49_003953"
     CDS             complement(1180..1638)
                     /locus_tag="L2X49_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBN4798960.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1751..2656
                     /gene="rssA"
                     /locus_tag="L2X49_003954"
     CDS             1751..2656
                     /gene="rssA"
                     /locus_tag="L2X49_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBN4798961.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            2748..3761
                     /gene="rssB"
                     /locus_tag="L2X49_003955"
     CDS             2748..3761
                     /gene="rssB"
                     /locus_tag="L2X49_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBN4798962.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3963..4871
                     /gene="galU"
                     /locus_tag="L2X49_003956"
     CDS             3963..4871
                     /gene="galU"
                     /locus_tag="L2X49_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBN4798963.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(5015..5428)
                     /gene="hns"
                     /locus_tag="L2X49_003957"
     CDS             complement(5015..5428)
                     /gene="hns"
                     /locus_tag="L2X49_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBN4798964.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            6032..6649
                     /gene="tdk"
                     /locus_tag="L2X49_003958"
     CDS             6032..6649
                     /gene="tdk"
                     /locus_tag="L2X49_003958"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBN4798965.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFAEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(6951..9626)
                     /gene="adhE"
                     /locus_tag="L2X49_003959"
                     /gene_synonym="adhC"
     CDS             complement(6951..9626)
                     /gene="adhE"
                     /locus_tag="L2X49_003959"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBN4798966.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            10103..10750
                     /gene="ychE"
                     /locus_tag="L2X49_003960"
     CDS             10103..10750
                     /gene="ychE"
                     /locus_tag="L2X49_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBN4798967.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGILPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         2797 a         2435 c         2680 g         2973 t
ORIGIN      
        1 taattacgga ttcgtttgag agttcaggga cttttgaaag tgatggtggt gggggaagga
       61 ttcgaacctt cgaagtcgat gacggcagat ttacagtctg ctccctttgg ccgctcggga
      121 accccaccac ggggtaatgc tttttactgg cctgctccct tatcgggaag cggggcgcat
      181 catatcaaat gacgcgccgc tgtaaagtgt tacgttgaga aaaatgaact ggttgcgtaa
      241 ttttcatccg taacggatta aaggtaacca gttatttttg ctggcgatta aagaataatc
      301 gtccgattac cgtaaacaaa gacgcgctgt gccagtactt tgtatagcgc acgacttaag
      361 acgtttttct cgacgtcacg acctgcgcgc atcatatctt cagctgtgta ggtatgatcg
      421 acatgaataa cgtcctgcat gatgattggg ccttcgtcca gattgtcatt cacatagtga
      481 gcggttgcgc caataatctt cacaccacgt tcataggcct ggtgataagg acgtgcgccg
      541 ataaacgcag gcaggaatga atggtgaata ttgatgattt tattcgggaa gcgtgccaca
      601 aattccggcg ttaatacccg catatacttc gccagcacca catagtcagg ttgataagca
      661 tcaatggcgt ccgccatctt ttgatcgtgc tcgttgcggc ttaacccttc atggcttacc
      721 agctcaaacg gaatatcaaa acgctcaacc agagaacgta aagtatcgtg gttaccaata
      781 actgccgcga tttcgacatc caggccgccg taattggctt tcatcaacaa atcgccaagg
      841 caatgcgctt ctttagtgac cagaatcact atccggcgac gaccggcagg attcagctca
      901 cgcacggagc cttctggcaa tgcgctatcg agatccgcca gaagggtgga atcattaaaa
      961 atcccttcca gttccgtgcg cataaaaaag cgcccggtac ggtgatcaac aaattcattg
     1021 ttctgtacga tatttaactc gtgcttgtag caaatattgg taatacgtgc gatcagacct
     1081 ttttggtccg gacaaatagt acgcagaact ttacgttgga gtgaatgcat tgctggaaaa
     1141 accttgttga gagtgtttgc taaaccgtac cgtcaaccat tattggccgc agcacttttt
     1201 aaatttttta cctgaaccac aagggcaggg atcgttgcga ccaaactgcg gacgtgtacc
     1261 gtcaatatag taccattgac cgttttcttt caaaaatcgc gaacgttcaa ttattgcacc
     1321 tgttttgcca ccttcggtaa atcgagcaac aaaactgaca aatccgatat tgtcggcgtc
     1381 ctgccaacaa tgttcaaata cggtcaatcc caaccattca gtatgcgcaa atccggccat
     1441 caattcggca cgtaacgccg ctgctccaca agagggatgc caggtcttta ttaaataatc
     1501 tgcgtcttgc atcacaaaag cgcagtaacg cgaacgcatg agatgttctg gatcaggtgc
     1561 aaccttttca ccagacacat aagggtggca acataggcta tactcgacag cactaccaca
     1621 gggacaaagc tgagacacaa ataatctccc tggaaacaat aacggcgtat taaccgcctg
     1681 agtagcacta tgttaaccga gcagtagcga tgtggctacg attgcattcc aggggaatct
     1741 tgcgggaata atgagaaaga taaaaatagg gctggcgctg ggatctggcg cggcgagagg
     1801 ttggtcgcat attggcgtta ttaatgcgct aaaaaaagtg ggtattgaaa ttgatatcgt
     1861 tgcaggatgt tcaattggtt cgctggtggg cgcggcctat gcatgcgatc gattatctgc
     1921 gctggaagat tgggtaacct ctttcagtta ttgggacgtt ttacgcctga tggatctctc
     1981 ctggcagcgc ggtgggttac tgcgcggcga gcgtgtcttc aatcaatacc gcgaaataat
     2041 gccggaaaca gagatcgaaa attgttcccg tcgctttgcg gctgttgcca ccaatttaag
     2101 tacgggacgt gaattatggt ttactgaagg cgatctccat cttgctattc gcgcatcatg
     2161 cagtattcca ggactcatgg cacctgttgc acataacggc tactggctgg ttgatggagc
     2221 agtcgttaac ccaattccta tttcactcac gcgtgcattg ggtgctgata ttgtgatagc
     2281 ggtcgacctg cagcacgatg ctcatttgat gcaacaagat ttgctctcct ttaatgtcag
     2341 tgaagaaaat agcgagaatg gtgattctct gccgtggcat gcgcgtctga aagaaaggtt
     2401 aggcagcata acgacacgtc gggcggtgac agcaccaacg gcaacagaga ttatgaccac
     2461 ttctatccag gtgctggaga accgccttaa aaggaaccgc atggcaggtg atccgcccga
     2521 tattctgatt caacctgttt gcccgcaaat atctacgctt gatttccatc gcgcgcacgc
     2581 tgccattgcg gccggacagc tggcagtgga aaagaaaatg gacgaacttt tgccgttggt
     2641 acgcaccaac atttgaccag aatttttatc tacacttaag ttaattctga caggcgcagg
     2701 tggcaatagc atgccactat tgagtaaagc cagtcagggg agagaacatg acgcagccat
     2761 tggtcggaaa acagattctc attgttgaag atgagcaggt atttcgctcg cttctggatt
     2821 cgtggttttc ctcattggga gcgacaacgg tactggcggc tgatggggtg gatgcccttg
     2881 agttgctggg aggtttcact ccagacctga tgatatgtga tatcgcgatg ccacgaatga
     2941 acgggcttaa actgctggag catatacgta acagaggcga ccagacccca gttctggtga
     3001 tatctgccac tgaaaatatg gcagatattg ccaaagcgtt acgtctgggc gttgaagatg
     3061 ttttgctgaa accagttaaa gatctgaatc gcttgcgcga gatggttttt gcctgtctct
     3121 atcccagcat gtttaattcg cgcgttgagg aagaggaaag gctttttcgc gactgggatg
     3181 caatggttga taaccctgcc gcagcggcga aattattaca ggaactacaa ccgccggttc
     3241 agcaggtgat ttcccattgc cgggttaatt atcgtcaatt ggttgccgcg gacaaacccg
     3301 gcctggtgct tgatattgcc gcactttcgg aaaacgatct ggcattttat tgccttgatg
     3361 taacccgagc tggacataat ggcgtacttg ctgccttgtt attacgcgca ttgtttaacg
     3421 gattattaca ggaacagctt gcacaccaaa atcaacggtt gccagagttg ggcgcgttat
     3481 tgaagcaggt aaaccattta ctccgtcagg ccaatctgcc ggggcagttt ccgctattag
     3541 ttggctatta tcatcgcgaa ctgaaaaatc tcattctggt ttctgcgggt ctgaatgcga
     3601 cgttaaatac cggcgaacac caggtgcaaa tcagtaatgg tgttccgtta ggcactttag
     3661 gtaacgctta tttgaatcaa ttgagccagc gatgcgatgc ctggcaatgc caaatatggg
     3721 gaaccggtgg tcgactgcgc ttgatgttgt ctgcagaatg agcaaacgat aacgcgggct
     3781 aaatttgcat tacctgctaa tgtcggctgg tggtactatc gtcgccattc gtataagtaa
     3841 ttgtcttaat tatgctaact cgcctccttt tcagaactta gccccttcgg ggtgctgata
     3901 tactgggatg cgatacagaa atatgaacac gttcaaaaca cgaacagtcc aggagaattt
     3961 aaatggctgc cattaatacg aaagtcaaaa aagccgttat ccccgttgcg ggattaggaa
     4021 ccaggatgtt gccggcgacg aaagccatcc cgaaagagat gctgccactt gtcgataagc
     4081 cattaattca atacgtcgtg aatgaatgta ttgcggctgg cattactgaa attgtgctgg
     4141 ttacacactc atctaaaaac tctattgaaa accactttga taccagtttt gaactggaag
     4201 caatgctgga aaaacgtgta aaacgtcaac tgcttgatga agtgcagtct atttgtccgc
     4261 cgcacgtgac tattatgcaa gttcgtcagg ggctggcgaa aggcctggga cacgcggtat
     4321 tgtgtgctca cccggtagtg ggtgatgaac ctgtggctgt tattttgcct gacgttattc
     4381 tggatgaata tgaatccgat ttgtcacagg ataacctggc agagatgatc cgccgctttg
     4441 atgaaacggg tcatagccag atcatggttg aaccggttgc tgatgtgacc gcatatggcg
     4501 ttgtggattg caaaggcgtt gaattagcgc cgggtgaaag cgtaccgatg gttggcgtgg
     4561 ttgaaaaacc aaaagcggat gttgcgccgt ctaatctcgc tattgtgggt cgttacgtac
     4621 ttagcgcgga tatttggccg ttgctggcaa aaacccctcc cggagctggt gatgaaattc
     4681 agctcaccga cgcaattgat atgctgatcg aaaaagaaac ggtggaagcc tatcatatga
     4741 aagggaagag ccatgactgc ggtaataaat taggttacat gcaggccttc gttgaatacg
     4801 gtattcgtca taacaccctt ggcacggaat ttaaagcctg gcttgaagaa gagatgggca
     4861 ttaagaagta acatccgtat cggtgttatc cacgaaacgg cgttgagcaa tcgacgccgt
     4921 ttttttatag cttattctta ttaaattgtc ttaaatcgga caataaaaaa tcccgccgat
     4981 ggcgggattt taagcaagta caatctacaa aagattattg cttgatcagg aaatcgtcga
     5041 gggatttacc ttgctcatcc attgcttttt tgattacagc tggagtacgg ccctggccag
     5101 tccaggtttt agtttcgccg ttttcgtcaa cgtagctata ttttgccgga cgctgagcac
     5161 gtttagcttt ggtgccagat ttaacggcag caaggctatt cagcagttcg ttcgggtcaa
     5221 taccgtcagc gatcagcatt tcgcgatatt gctgcagttt acgagtgcgc tcttcaactt
     5281 cagcagcagc cgcgctttct tcttcgcgac gttcgttaac aacaacttct aatttttcca
     5341 gcatttcttc cagcgtttca agtgtacatt ctcttgcctg cgcacgaaga gtacggatgt
     5401 tgttcagaat tttaagtgct tcgctcattg tagtaatctc aaacttatat tggggtggtt
     5461 tgttgaggta ataatagagc cttaaattca gttgtgcaat agccaggaat gtaaggaatt
     5521 caaaattgtt ctttattttg tgccgccaat aaatatcttt tcataaaatt agccagaaaa
     5581 gacgcggcat atagccctat ttgcaccgat gatttcgcag ctcgtgaggt taaaacttcc
     5641 tgattcatgt cacattttat ggggagatta tcgtaggctg acgacctttc agtcttctgt
     5701 attagtcgtg tttacgagaa ttccctatta agcgaatgat gaaaagtaga acagtcgcaa
     5761 taagagcagg gacttagtat tgcactatct cctggaggtc aacagagggc tattacttgc
     5821 gcaacaggtt aaagattgtg aatagttacc agcagtcatt tacccgctta taacaagcga
     5881 ggcaatagta atgatagctc agaaggatta tgcaaggctt cgtaagggag aacgcatata
     5941 ccacttctgt gcatactgtt gagctgaaaa actgacgaat tatgataaac tccagccaac
     6001 gttatttcat atcattgagg gcctgtggct gatggcacag ctatatttct actattccgc
     6061 aatgaatgcg ggtaagtcta cagcattgtt gcaatcttca tacaattacc aggaacgcgg
     6121 catgcgcact gtcgtatata cggcagaaat tgatgatcgc tttggtgccg ggaaagtcag
     6181 ctcgcgtata ggtttgtcat cgcctgcaaa attatttaac caaaattcat cattatttgc
     6241 tgagattcgt gcggaacatg aacagcaggc aattcattgc gtactggttg atgaatgcca
     6301 gtttttaacc agacaacaag tatatgaatt atcggaggtt gtagatcaac tcgatatacc
     6361 cgtactttgt tatggtttac gtaccgattt tcgaggtgaa ttatttattg gtagccaata
     6421 cttactggca tggtccgaca aactggttga attaaaaacc atctgttttt gtggccgtaa
     6481 agcaagcatg gtgctgcgtc ttgatcaagc aggcagacct tataacgaag gtgagcaggt
     6541 ggttattggt ggtaatgaac gctacgtttc tgtatgccgt aaacactata aagaggcgtt
     6601 acaagtcggc tcattaacgg ctattcagga aaggcatcgc cacgattaat aagaatttct
     6661 ttactgacag ggtgagcagg gcacttttat cctgtcagtt cgttttacgc acttcttccg
     6721 ggctatacac ccttctcggc agttttttaa cgccgctata cgcctcacag ggctcttaag
     6781 caccgacgtt gacttgtgac ctgtaaagta caatatccct gtgtttacgc gttatacatc
     6841 gtcgcaaata tgatgaaggc taatgctgtc ggtttatgga aaagttgctt tgggtaaaca
     6901 aaaaaacggc cccagaaggg gccgtttatg ttgccagaca gcgctactga ttaagcggat
     6961 tttttcgctt ttttctcagc tttagccgga gcggcttctt ttttcgctgc agtttcacct
     7021 tctacataat cacgaccgta gtaggtatcc agcagaatct gtttcagctc ggagatcagc
     7081 gggtaacgcg ggttagcgcc ggtgcactgg tcatcgaatg catcttcaga cagtttatcc
     7141 acgttcgcca ggaagtctgc ttcctgaacg ccagcttcac ggatagattt cggaataccc
     7201 agttcagcct tcagcgtttc cagccatgcc agcagtttct cgatcttagc agcagtacgg
     7261 tcgcccggtg cgctcagacc caagtggtcg gcaatttcag cataacgacg gcgagcctgc
     7321 ggacggtcat actggctgaa tgcagtctgc ttggtcgggt tgtcgttcgc attgtagcga
     7381 ataacgttac aaatcagcag ggcgtttgcc agaccgtgcg gaatatggaa ctgggaaccc
     7441 agtttgtgcg ccattgagtg acatacaccc aggaaggcgt tcgcaaacgc gatacccgcg
     7501 atagtcgctg cactgtgaac acgttcacgc gctaccggat ttttagaccc ttcgtggtag
     7561 gacgctggca gatattcttt cagcagtttc agtgcctgca gagcctgacc atcagagaac
     7621 tcagatgcca gtacagaaac ataagcttcc atggcgtgag ttactgcgtc cagaccaccg
     7681 aaagcacaca gggacttcgg catgtccata accaggttgg cgtcgacaat cgccatatcc
     7741 ggagtcagcg catagtctgc cagcggatat ttctgaccag tagcgtcgtc agttacaacc
     7801 gcaaacggag tgacttcaga acctgtacca gaagtggtgg tgacagcgat cattttcgct
     7861 ttcacgccca ttttcgggaa cttgtagata cgtttacgga tatccataaa gcgcagcgcc
     7921 agctcttcga agtgagtttc cggatgttcg tacataaccc acatgatctt cgcggcgtcc
     7981 atcggggaac caccacccag cgcgataatc acgtctggtt tgaaggagtt tgccagttct
     8041 gcacctttac gaacgatgct cagggtcggg tccgcttcta cttcgaagaa gacttcagtt
     8101 tcaacgcctg ctgctttcag tacggaagtg atctgatcag cataaccatt gttgaacagg
     8161 aagcggtcag tcacgatgag cgcacgtttg tggccatcag taatcacttc atccagcgcg
     8221 attggcaggg agccacggcg gaagtagata gatttcggaa gtttgtgcca caacatgttt
     8281 tcagctcgct tagcaacggt tttcttgttg atcaggtgtt tcggaccaac gttttcagag
     8341 atggagttac caccccaaga accacaaccc agagtcaggg aaggtgcgag tttgaagtta
     8401 tacaggtcac cgataccacc ctgagacgct ggggtgttaa tcaggatacg agccgttttc
     8461 attttctgac cgaagtaaga aacgcgagcc ggttggttat cctggtcagt gtacaggcaa
     8521 gaggtatgac cgataccgcc catagcaacc agtttctctg ctttttctac cgcgtcttcg
     8581 aaatctttag cgcggtacat tgccagagtc ggggacagtt tttcatgtgc gaacggttcg
     8641 ctttcatcaa caacggtcac ttcaccgatc agaatcttgg tgttttctgg tacagagaag
     8701 cctgccagtt cagcaatttt ataggctggc tgaccaacga tagccgcgtt cagcgcaccg
     8761 tttttcagga taacatcctg aacagctttc agctctttac cctgcaacag atagccgccg
     8821 tgggttgcaa aacgttcacg tacagcgtca taaacagagt caacaacaac aacagactgt
     8881 tcagaagcac agattacgcc gttgtcgaag gttttggaca tcagtacaga tgcaactgca
     8941 cgtttgatat cagcagtttc atcgataaca actggagtgt tgcccgcgcc tacaccgata
     9001 gctggtttac cggagctgta tgcggcttta accatgcccg gaccaccagt cgcgaggatc
     9061 aggttgatgt ctgggtggtg catcagtgcg ttagacagtt caacagaagg ttgatcgatc
     9121 cagccgatca gatctttcgg agcaccggca gcgatagcag cctgcagaac gatatcagcc
     9181 gctttgttgg tggcatcttt tgcacgcggg tgcggggaga agataatggc gttacgggtc
     9241 ttcagactga tcagcgattt gaagatagca gttgaagtcg ggttagtggt cggaacgata
     9301 ccgcaaataa taccgattgg ttcagcgata gtgatggtac caaaagtgtc gtcttcagac
     9361 agaacaccac aggttttttc atctttatag gcgttgtaga tatattcaga agcaaagtgg
     9421 tttttgatca ctttatcttc gacgataccc atgccggatt cggcaacggc cattttcgcg
     9481 agtgggattc gagcatctgc agcagccaga gcggcggcgc ggaagatttt gtctacttgc
     9541 tcttgagtga aactggcata ttcacgctgg gcttttttta cacgctctac gagtgcgtta
     9601 agttcagcga cattagtaac agccataatg ctctcctgat aatgttaaac ttttttagta
     9661 aatcatctgc tcgaatacga gagtatagtc agtgcggtga tgatttgctt aacctatgaa
     9721 aatcaaaagc ttactcgcgc tcacactcac tgtgatttac taaaagagtt taaacattag
     9781 agttattatc tctaatgcgt cacttccagg tggcgtaagc aagattactc acttctgggt
     9841 actgattacg tgatccaaat caaatttttg caaagctgac acctttcagc atcgcttttc
     9901 gccattatag ctaacagtta ataaattgta gtatgatttg gtggctacat tagcatgttt
     9961 tgcacaacta gataacaata acgaatgata gcaattttaa gtagttagga ggtgaaaaat
    10021 gctgtcaaaa ggcgtattgt cagcgcgtct tttcaacctt atttatggct aacattatcc
    10081 ggcttttgct tcggagctaa ccgtgattca gacctttttt gattttcccg tttacttcaa
    10141 atttttcatc gggttatttg cgctggtcaa cccggtaggg attcttcccg tctttatcag
    10201 catgaccagt tatcagacag cggcagcgcg aaacaaaact aaccttacag ccaacctgtc
    10261 tgtggccatt atcttgtgga tctcgctttt tctcggcgac acgattctgc aactttttgg
    10321 tatatcaatt gattcgttcc gtatcgccgg gggcatcctg gtggtgacga tagcgatgtc
    10381 gatgatcagc ggcaagcttg gcgaggataa acagaacaag caagaaaaat cagaaaccgc
    10441 ggtacgtgaa agcattggtg tggtgccatt ggcgttgccg ttgatggcgg ggccaggggc
    10501 gatcagttct accatcgtct ggggtacgcg ttatcacagc attagctatc tgtttggttt
    10561 ctttgtggct attgcattgt tcgctttatg ttgctgggga ttgttccgca tggcaccgtg
    10621 gctggtacgg gttttacgcc agaccggcat caacgtgatt acgcgtatta tggggctatt
    10681 gctgatggca ttggggattg aatttatcgt tactggtatt aaggggattt tccccggcct
    10741 gcttaattaa ttcctttcaa atgaaacgga gctgccatgc tccgtttact tcgtcattat
    10801 ttttactttg ttcccgcgca gttatcaaaa gcaaaaggaa taggtaaaaa tattcttctc
    10861 aaattacagt tagttataag gattt
//
LOCUS       DAFNBJ010000157        10879 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000157 DAFNBJ010000000
VERSION     DAFNBJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.157"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            60..821
                     /gene="adiY"
                     /locus_tag="L2X49_003961"
     CDS             60..821
                     /gene="adiY"
                     /locus_tag="L2X49_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBN4798968.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            958..2295
                     /gene="adiC"
                     /locus_tag="L2X49_003962"
     CDS             958..2295
                     /gene="adiC"
                     /locus_tag="L2X49_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBN4798969.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2399..4042
                     /gene="eptA"
                     /locus_tag="L2X49_003963"
     CDS             2399..4042
                     /gene="eptA"
                     /locus_tag="L2X49_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBN4798970.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4039..4707
                     /gene="basR"
                     /locus_tag="L2X49_003964"
     CDS             4039..4707
                     /gene="basR"
                     /locus_tag="L2X49_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBN4798971.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4708..5808
                     /gene="pmrB"
                     /locus_tag="L2X49_003965"
     CDS             4708..5808
                     /gene="pmrB"
                     /locus_tag="L2X49_003965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBN4798972.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5784..5873)
                     /gene="pmrR"
                     /locus_tag="L2X49_003966"
     CDS             complement(5784..5873)
                     /gene="pmrR"
                     /locus_tag="L2X49_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBN4798973.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5985..7487)
                     /gene="proP"
                     /locus_tag="L2X49_003967"
     CDS             complement(5985..7487)
                     /gene="proP"
                     /locus_tag="L2X49_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBN4798974.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7751..8629)
                     /locus_tag="L2X49_003968"
     CDS             complement(7751..8629)
                     /locus_tag="L2X49_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBN4798975.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGVFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8626..10854)
                     /gene="crfC"
                     /locus_tag="L2X49_003969"
     CDS             complement(8626..10854)
                     /gene="crfC"
                     /locus_tag="L2X49_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBN4798976.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELVNNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSAVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2725 a         2844 c         2687 g         2623 t
ORIGIN      
        1 cccggtatcg ctttgcttta gcgagaactg gtcttttatt tgcactcagg agtacatgta
       61 tgaggatttg cagcgaccaa ccttgtattg ttttattgac tgaaaaagat gtctggataa
      121 gggtgaatgg gaaagaacct attagcctta aagctaacca tatggcgtta ttaaattgtg
      181 aaaataatat tatcgacgtc tcctctctta acaacacttt ggttgctcat attagtcacg
      241 acatcatcaa agattacctc cggtttctga ataaagatct ctcgcaaata ccagtatggc
      301 aacgtagcgc tacgcccata ctcaccctgc catgcctgac gccagacgtc tttcgcgttg
      361 ccgcgcaaca cagcatgatg cccgcagaaa ctgagtcaga aaaggaacga acacgcgcat
      421 tattattcac ggtgctatcc cgttttctcg acagtaaaaa attcctttca ttaatgatgt
      481 atatgttacg taattgtgta agtgacagcg tttatcaaat tattgaaagc gatattcaca
      541 aagactggaa tcttagtatg gtagccagtt gtttatgtct tagcccaagt ctgttaaaga
      601 aaaagttgaa aagcgaaaac accagttata gccaaataat caccacctgc cgcatgcgtt
      661 atgccgtaaa tgaattaatg atggacggta aaaatatctc tcaggtatca cagtcctgcg
      721 gctacaacag tacgtcgtac tttatttctg tctttaaaga cttctacggt atgacgccgc
      781 tgcattatgt tagtcagcac agagaacgca ctgtcgcctg atttttaacc ttaacgaaga
      841 gctatattaa taacggcatc agcgataacc cggtcgataa taattcaact atcgaatgca
      901 ggcgtatgat atgacgtaat ttattgtcac gaagctcgcc ttcgcaggag tttaattatg
      961 tcttcggatg ctgatgctca caaagtgggc ttaatccccg tcaccctgat ggtgtcgggg
     1021 aatattatgg ggtcaggtgt ttttctgtta cctgcaaacc tggcctctac tggcgggatt
     1081 gccatttatg gatggttggt gacgattatc ggtgcgctgg ggctctcgat ggtatacgcc
     1141 aaaatgtcgt tcctcgatcc aagtcctggt ggttcttacg cgtacgcccg ccgctgcttt
     1201 ggcccgtttc tcggttatca aaccaacgtc ctctactggc tggcctgctg gatcggcaat
     1261 atcgccatgg tggtcattgg cgtaggatat ttaagttact tcttcccgat tctgaaagat
     1321 ccattggtat taaccatcac ctgcgtcgtg gtgctgtgga tcttcgtcct gctgaacatt
     1381 gtcggtccga aaatgatcac ccgtgtgcag gcagttgcca ccgtgctggc gctgatcccc
     1441 atcgtcggga ttgccgtatt tggctggttc tggttccgtg gtgaaaccta tatggcggca
     1501 tggaacgtca gcggcctggg caccttcggt gcaattcaaa gtacccttaa cgttacgctg
     1561 tggtcgttca tcggtgtgga aagtgcctcc gttgccgcag gtgtggtgaa aaacccgaaa
     1621 cgcaatgtcc ctatcgccac cattggtggg gtattgattg ccgccgtttg ctatgtactt
     1681 tctaccaccg cgattatggg gatgatccct aatgccgcac tgcgcgtttc tgcttcgcca
     1741 ttcggtgatg ccgcacggat ggcgttgggt gacaccgccg gggccattgt ttccttctgc
     1801 gcagctgcgg gttgcttagg ttcactgggc ggctggacgt tgctggcggg tcaaacggcg
     1861 aaagccgctg ccgatgacgg actgttccca ccaatttttg cccgtgtaaa taaagcgggt
     1921 acgccagtgg cggggctgat tatcgtcggt attttgatga ccatcttcca gctcagcagc
     1981 atttcaccaa acgcgaccaa agagttcggt ctggtttctt ccgtctcggt catctttaca
     2041 ctggtgccat atctttacac ctgtgcggcg ttactgctgc tcggacacgg tcactttggt
     2101 aaagcacgcc cggcatatct ggcagttact accattgcct tcctctactg catctgggcc
     2161 gtggtggggt ccggagcgaa agaggttatg tggtcatttg tcaccctgat ggtcatcacc
     2221 gccatgtatg cgctgaatta caaccggcta cataaaaacc cgtatccctt agatgcacca
     2281 ataagcaaag attaattccc cttaatccag caaacataaa agccaacctt aagaacttaa
     2341 ggttggctta attttgcttt gcgagcatat gcgcactttg ttcgatggaa acaccgtgat
     2401 gttgaagcgc ctactaaaaa gaccctcttt gaatttactc gcctggctat tgttggccgc
     2461 tttttatatc tctatctgcc tgaatattgc cttttttaaa caggtgttgc aggcgctgcc
     2521 gctggattcg ctgcataacg tactggtttt cttgtcgatg ccggtcgtcg ctttcagcgt
     2581 gattaatatt gtcctgacac taagctcttt cttatggctt aatcgaccac tggcctgcct
     2641 gtttattctg gttggcgcgg ctgcacaata tttcataatg acttacggca tcgtcatcga
     2701 ccgctcgatg attgccaata ttattgatac cactccggca gaaagttatg cgctgatgac
     2761 accgcaaatg ttattaacgc tgggattcag cggcgtgctt gctgcgctga ttgcctgctg
     2821 gataaaaatc aaacctgcca cctcgcgtct gcgcagtgtt cttttccgtg gagccaatat
     2881 tctggtttct gtactactga ttttgctggt cgccgcactg ttttataaag actacgcctc
     2941 gttgttccgc aataacaaag agctggtgaa atccttaagc ccctctaaca gcattgttgc
     3001 cagctggtca tggtactccc atcagcgact agcaaatcta ccgctggtgc gaattggtga
     3061 agacgcccac cgcaacccgt taatgcagaa cgaaaaacgt aaaaatttga ccatcctgat
     3121 tgtcggcgaa acctcgcggg cggagaactt ctccctcaac ggctacccgc gtgaaactaa
     3181 cccgcggctg gcgaaagata acgtggtcta tttccctaat accgcatctt gcggcacggc
     3241 aacggcagtt tcagtaccgt gcatgttctc ggatatgccg cgtgagcatt acaaagaaga
     3301 gctggcacag caccaggaag gcgtgctgga tatcattcag cgagcgggca tcaacgtgct
     3361 gtggaatgac aacgatggcg gctgtaaagg tgcctgcgac cgcgtgcctc accagaacgt
     3421 caccgcgctg aatctgcctg gtcagtgcat caacggcgaa tgctatgacg aagtgctgtt
     3481 ccacgggctg gaagagtaca tcaataacct gcagggtgat ggcgtgattg tcttacacac
     3541 catcggcagc cacggtccga cctattacaa ccgctatccg ccgcagttca ggaaatttac
     3601 cccaacctgc gacaccaatg agatccagac ctgtagcaaa gagcaactgg tgaacactta
     3661 cgacaacacg ctggtttacg tcgactatat tgttgataaa gcgattaatc tgctgaaaga
     3721 acatcaggat aaatttacca ccagcctggt ttatctttct gaccacggtg aatcgttagg
     3781 tgaaaatggc atctatctgc acggtctgcc ttatgccatc gccccggata gccaaaaaca
     3841 ggtgccaatg ctgctgtggc tgtcggagga ttatcaaaaa cggtatcagg ttgaccagaa
     3901 ctgcctgcaa aaacaggcgc aaacgcaaca ctattcacaa gacaatttat tctccacgct
     3961 attgggatta actggcgttg agacgaagta ttaccaggct gcggatgata ttctgcaaac
     4021 ttgcaggaga gtgagtgaat gaaaattctg attgttgaag acgatacgct gttattgcag
     4081 ggattgattc tggcggcgca aaccgaaggc tacgcgtgcg atggcgtgac aaccgcgcgg
     4141 atggcggaac aaagccttga ggccggtcat tacagcctgg tggtactgga tttagggtta
     4201 cccgacgaag atggactgca ttttctcgcc cgtatccggc agaaaaaata caccctgcca
     4261 gtactgatcc tcaccgctcg cgatacgctg accgacaaaa tcgccgggct ggatgtcggt
     4321 gccgatgact atctggtgaa gccttttgcg ctggaagagt tacacgcccg tatccgcgcc
     4381 ctgctacgac gccataataa tcagggcgaa agtgagctga ttgttggcaa tctgacgctg
     4441 aatatgggtc gccgtcaggt atggatgagc ggtgaagagt tgattctgac gcccaaagaa
     4501 tatgctctgc tgtcacggtt aatgctcaaa gcaggcagtc cggtgcatcg ggaaattctc
     4561 tacaacgaca tctataactg ggacaatgaa ccctcgacca acaccctgga agtgcatatc
     4621 cacaatctgc gcgacaaagt gggcaaagcc cgtatccgca ccgtgcgcgg ctttggctat
     4681 atgctggtcg cgaatgagga aaactaattg aatctgatgc attttctgcg ccgaccaata
     4741 tcgctgcgcc aacggctgat attgaccatc ggggccattt tgttggtgtt tgagctgatc
     4801 agcgtcttct ggctatggca tgaaagtacc gagcagattc agctgtttga gcaggcgcta
     4861 cgcgacaatc gcaacaacga tcgccacatc atgcgtgaga tccgcgaggc ggtcgccagc
     4921 ctgattgtcc ccggcgtctt tatggtcagc ctgacgctat ttatctgcta tcaggcggta
     4981 cgccgcatca cccgcccgct ggcggagctg caaaaagagc tggaagcgcg caccgccgac
     5041 aacttaacgc ccattgccat tcacagcgcc accctcgaaa tcgaagcggt ggtttcggcg
     5101 ttgaacgatc tggtcagtcg cctgaccagc acgctggata acgaaaggtt gtttaccgct
     5161 gacgtcgcgc acgaactgcg aacgccactg gcgggggtgc gtttgcatct ggaactgctg
     5221 gcgaaaacgc atcacattga tgtagcaccg ttagtggcac ggcttgatca gatgatggag
     5281 agcgtctccc agctgctgca actggcgcgt gccggacagt cattttcttc cggtaattat
     5341 caacatgtaa aactgctgga agatgtcatt ctcccctcgt atgacgaact cagtaccatg
     5401 ctcgaccagc gacagcaaac cctgctattg ccagagagcg ccgccgacat caccgttcag
     5461 ggtgatgcaa ccctgctgcg gatgttattg cgaaacctgg tagaaaacgc ccatcgttac
     5521 agcccgcaag gcagcaacat tatgattaag ctgcaagaag atggcggagc ggtcatggca
     5581 gttgaagatg aaggaccagg tattgatgag agtaaatgcg gggagttgag taaagcgttt
     5641 gtacgtatgg acagccgtta tggcgggatt ggtctggggt taagtattgt cagccgcatt
     5701 acacagttgc atcacgggca gtttttcctg caaaaccggc aagagacttc cggcacgcgg
     5761 gcctgggtac ggctgaagaa agatcagaac gtggcaaacc agatataaag aaagctgctg
     5821 accaccagca cgctgaacac ggtagttaaa ctttcataaa cacggttttt cattgcactc
     5881 tcctccgtta acctggagga gagtatgcgc gtcagagatt aagtgaacct taagagttca
     5941 attggcctga taagacagcg tcacatcagg ccatccgttt cagcttattc atcaattcgc
     6001 ggatgttgct gcaccaggcg ggtacgtttc gcctgcaaat cggcaatctc gtggtcaata
     6061 ttatcgattt tctgctcgat attatcgtaa tgctcgacga gaatttcctt cgcttcctgt
     6121 atatctgacg ccgccggtgt cgcacctttc aacggacgat ttgccgtctc tttcatggtt
     6181 acgccggtga ttaaaccaat caccgccact accatcaggt aataggcagg catcatcaga
     6241 ttctgcgagc tttcgaccag ccaggccgcc agtgttggcg tcagaccggc aaccagcacc
     6301 gaaatattaa atgccgccgc cagcgcgctg taacggatat gcgtcgggaa catcgctggc
     6361 aaggtagaag ccataacgcc cgtaaagcag ttaaggatca ccgccagcat cagtaacccg
     6421 gcaaaaatca ggccgatgac gttactgtta atcagaataa acgccgggat cgccaacaca
     6481 aacagggcaa cactaccaag tagcacaaac ggacgacggc caaaacggtc actcagcaag
     6541 cccatcaccg gctggacaaa cagcataccg atcataatgg cgataataat cagcaccccg
     6601 tggtcttcgg agtaatgcag gttatgcgac aaataactcg gcatataggt cagcaacatg
     6661 tagtaagtca cgttggtggc aattaccaga ccaatacatg tcaacaggct gcgccagtgt
     6721 ttggtggcaa tctctttaaa cgagactttc gggccatcct gcaaaccttc gcggtcgccc
     6781 tgttccagtt tatcgacatg ctgctggaac gccggagttt cttccagcgc atggcgcagg
     6841 taaagcccga taatccctaa cggcagagca ataaagaacg gaatacgcca gccccagtcg
     6901 aggaagttcg cttcgccgac aatggtcgaa attaacacca ctacgccagc acccagcaca
     6961 aacccggcaa tagaaccgaa gtccagccag ctgcccataa agccacgttt acggtcaggt
     7021 gagtattccg caacaaatat cgacgccccg gtatattcac cgccgaccga gaaaccttgt
     7081 gccatcttac agatcaacag cagaatcggt gcccaaatac caatcgtgtc gtaggacggt
     7141 attaagccaa tacagaacgt actgatcgac ataatcacaa tagtgatagc gaggatcttc
     7201 tggcgaccat atttatcgcc caacatacca aagaagagcc cgccaagcgg tcgaatcaga
     7261 aagggaacgg agaaagtggc aagtgcagca accatctgca cgctggggtc agcccccggg
     7321 aaaaaaactt tacctaatgc gtaagcaaca aaaccataaa caccaaaatc aaaccattcc
     7381 attgcattac ccagtgatgc tgcggtaatg gctttacgca gtttaccgtc atcaataatg
     7441 gtgacatcac gaagggtaat tggttttact tttttccttt tcagcatagc tttcctcgca
     7501 gagttggccc atttccgcaa acgccagaaa aatcagcgcg acggccgcaa aaggggagcg
     7561 ggaattgcag gatgcaatct ctgtaacaag cgtagcgggt ttgcttacac cctccggtta
     7621 aggaatgtac aatcaaacca gttgacgcct gaattgggca gttaatgacc tttttaccct
     7681 accagtgttt aaatttgtga tcaacttcac acatggattt cgtttaggac tcattgatgt
     7741 aactgattat ttacgccaaa tttacagaga agatttcctc gagcattgcc cccgccagac
     7801 gctgcgctga acagagtcgt ggcatcccca gcgcgacagt ttgccagcgt tgggtcactg
     7861 accagcaaac gggttgttta tctgccgtgg tccactttcc cagccaactt aaaaaatcag
     7921 catgatcccc gcccgtcgga acggtcgaag aggcaaacca ctgatggtaa tagtgacgcg
     7981 tggtcggggc cgcattgata ctctctgcca gatgttgtgt cgctttcacg cgcaaatcac
     8041 ccagcaacaa cgccaccgct tgcgggcttc ccagacgaca ggcatcgcca aatacccccc
     8101 aacgcaactc ttcgagaacg ataaaacagc gcccaggcaa cgaccaggcg ttatacgccc
     8161 cagcacgcca gcgggtaaaa atttgctcgc tctgttcacc cgcctgcacc agtaggcctc
     8221 gttgcgttaa tgccttttcc ttccagcgac aacgggctga aaaccactca tacaaggcgg
     8281 caatttgctg ggggagctcc gggaaagcag agtgctggcg gtgatccatt ttttgcagca
     8341 gcgccagatg ctgcgcgatc tgcgtcagcg ccagatgacc gggatccagc atcgacgcca
     8401 gtttttccac cattgtcagt cgcaggctaa gcggcgcatt taacatgccg ccgttcgtcc
     8461 atgtctgaag ctgcacacgc gaaagaagtt cctgcatcag tcgttcacga aactgctgct
     8521 gatgggcttg tggaaggcgg gcatcatcgc cctgcgccag atccactaaa aagcggggat
     8581 aaaccgactc cagcacgcga ccggggccgt ccagtaacgt cttggtcaat atcgttctgc
     8641 cgtgaaaagt gtttgaatat catcgcgtaa cagctgggcg tcggtgtaaa tccagccgtg
     8701 agtcatcaca gtctgctgca attgctgctg catcagcctg accaccgatt cattttgttg
     8761 acgcagagcc aggctttcgc gtaaacgcgt ctgtaattcc gtcaaacatg aagcgaactc
     8821 agcgaaaaaa gtattcatgc ctgccgtaac agattcatcg acctgctctg ccagaacttt
     8881 agccatttgt tggcaataaa gatcgacttc tgcgcttaat gctcgttgca acacactgta
     8941 atcaaccgtt tctgtcgggg atttctcatt tccccgtccc cagtcgggct gattcaacca
     9001 gcgcgaaaaa gtctcacgca caacgcctaa acgcgtgctc tgctcgtccg ttgcatcctg
     9061 gcgcgaaatg actgcactga acagctggcg agtgttgaag tggggaacta cgccgtgaaa
     9121 aacaggaaaa tgaaacccag gacgaaaccc tgactcgctc aattccattt tgacttgttg
     9181 ctcaatgggg cgaataacat cggttaacac tcggcaaagg gtggattcca gctcggcaaa
     9241 acgcagcgta aagtcgcgac tgatggtgtt ctgcgccgtc tgtaacagtg tctcacagcg
     9301 ggtacgcatc tcgcttaacg gctccgaatc atcctgaaac aaggcggcta actgcgcatt
     9361 cagcgcatct tgttgttgac gcagaaagtg gttggcggag gtcagggcca gctcgatttc
     9421 atgtttaatc tcgccgctca cctgcgcctg attgagttgc aatagctgca aactttcttc
     9481 gacctgatgg atattttgcc gcaattgttc acaagcgacg tttaacccgt gcgcacgaaa
     9541 atccaggtat tcccgcgcct gctgcgcgta attcaacagt ttatgcgcag cagatcgcaa
     9601 agcatacaac gaggcgttag cgtaagcggc atgaagcaac gcctgaattg gctgggcgaa
     9661 cagcgaatct tcccacaact gatcggcagc atgacgaata tgttcgaggt ccgccagatc
     9721 ggcatgacgc cagcgcctgc cgagcgcggc atgggcaaaa tcttccaccc agcgttgttg
     9781 ctctggcgct ggtaacttac cgttgttgac taactcatgg cgcgcccgat tcgccaggta
     9841 gccccacatc gacgacaccg gaaatatctg ctgtggcgta atacagcctt tcatcagcgt
     9901 cccggaaatc agtgcccgca cctggtcggc gtcgtcactg ttacgatcct gttgatcgaa
     9961 cttattgacc agcacataca gcggcaccga ttgccccacc gccaaaatcg cctcacggac
    10021 ctcttcatcg gagatcgatt tcagttgcgt ataatccagc accgccagta ccgccgaggc
    10081 gcgtgccagc tgctggttaa gcattttttg cagatgcggt tgcccggctt catttggccc
    10141 gggggtatcc agtaacgtca actgaccggg ataactctcc agccccgcca gatggacaaa
    10201 ctcaacttca atcacgggaa tatgctcaat ggcggcgtaa gcagaaaaag gaaaatcgac
    10261 gtccagcgcc ttcgccagtc gcactaaatc attcaaactt ttcagacaat gaaaaatagg
    10321 ctgggcaccc agataatatt tttcgaaagc gacgccattt tcgatccgct gcataagcgc
    10381 acgcatatct ttatctattt ccagcacatc ggtcagatgc ttaatatcgc aatcacgcag
    10441 gcgctgttgt aattgttgaa ttaaacaatc gattggcgcg acatgtgaaa aatgcagtac
    10501 cggttccttt tgcccgggcg tatggcgaat aagcgtcggc agcgcagtca ttgggcgatt
    10561 acgattaggc agaacctccg taccaacaat ggcattaatg gtggttgatt tccctgcttt
    10621 catggtaccg acaattgcca gcaccatttc cagtcgggaa attttacgca actcattatt
    10681 cagcatcgcg tgacgttcgg cgatattagg ctgactccag ggtaaagcca gttgtggcgc
    10741 gtcgtctccg ggtacagaga gaggcatttt ttccagtaac tgcaactgtt ggcgagaaag
    10801 ctgtaacagg cgttcagcct cctgacttaa ctcatacagg gtctgtgtgt acatagaaaa
    10861 ttcttcctta aagcaaatt
//
LOCUS       DAFNBJ010000158        10864 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000158 DAFNBJ010000000
VERSION     DAFNBJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.158"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            954..1307
                     /locus_tag="L2X49_003970"
     CDS             954..1307
                     /locus_tag="L2X49_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HBN4798977.1"
                     /translation="MELTLNTNVERGKTNAFAFLKSKHAKVPLFILLVLANCSFAYAG
                     SDDGAFGDIWAYMSEALTGAPGKIIACGMLFSVAYFGVVKPNLGLALVSALMMLVMAN
                     GEKIISSFLDAGIPL"
     gene            1326..1676
                     /locus_tag="L2X49_003971"
     CDS             1326..1676
                     /locus_tag="L2X49_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HBN4798978.1"
                     /translation="MRGIFPLSGVFMTSQYEIPPHTYRFPYRINMPLLILFWDAKQLG
                     ITFVTIASGNIFDFFITSVVVAVVFWFAYKKAAEEGIRGKLKHKLWWYGFFPGKSVFS
                     SRYFTDPFIRNLYS"
     gene            1688..2473
                     /locus_tag="L2X49_003972"
     CDS             1688..2473
                     /locus_tag="L2X49_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HBN4798979.1"
                     /translation="MKLLSRLKIPFIKNSSNGDFDNKDETSQKGGEEVKGSFLDSKAR
                     FSKEIEGSEIGITYSALIKRDEKLLRVNTVAMIIIGVLVVKNQFLTDPVTIVLPPNMT
                     EEVKVVGNKASESYKTQWALFFSTLIGNINPTNIGFVTTTILDALSPDLQAKTRESLQ
                     QQTNIMQARGVEQSFKPIDMYYDTKNDMVYVWGTKSTRLINVPDKTESSKWTFEWVLG
                     MKNGRPRIAYVNQYSGTPNIKKITINGKEQLATLDNPPPSIGN"
     gene            2476..3708
                     /locus_tag="L2X49_003973"
     CDS             2476..3708
                     /locus_tag="L2X49_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HBN4798980.1"
                     /translation="MTSKKVFHCTAITAVILLATSNVVFAEDYQLPATVNNPVVMPVG
                     ADEFQNGVKNAIIKDTGTTTETVQATKPTTALPSLSPASSASPAVNEITGALSNNPTL
                     AGYQQQVKSGNFDSYGRPAGKEPQQNAQSTGSPSKADELYVEARNRYKEVQRVNVPPG
                     GNIVLPVSRGLQNRISTSFKNASVSTSTPADEASIFVNGGDVFISTNTDKPIGIMLSE
                     DSVPESTYNLTLVPLDVPGAMISVTTSLSPTMQAKRETSLDKQNYDEMLARSQSEELT
                     PSDPRQDDHKQRIIDLLTPVALGEVPSGFSLQEDRLSRIPSSEQSPCNFNMYAKLGQR
                     LVGSRELIDVVLVKNDKPYGQVVADQQCITEGVVASALFDKAFLQPGEETELYIVRDK
                     LFKERQTRVTTRPSLIKR"
     gene            3710..4150
                     /locus_tag="L2X49_003974"
     CDS             3710..4150
                     /locus_tag="L2X49_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798981.1"
                     /translation="MRKATFLTVISFGLVVFLGTSYYYQTKYIPKGTVNIFPDVHRQG
                     EIVDDAFGKSTIVISDPTLADKFVGSSTESVGRHEVNVIKELGTQNNGSKFVHDESDK
                     SSLTVGDKWPRAGEPYIVPQMTEYERNLKVKRFQQPKNGVNNGH"
     gene            4140..5498
                     /gene="trhB"
                     /locus_tag="L2X49_003975"
     CDS             4140..5498
                     /gene="trhB"
                     /locus_tag="L2X49_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhB"
                     /protein_id="HBN4798982.1"
                     /translation="MDIKKAWENKTVRISVIGAALMVLIVVISQSIFTTPVKKEKKTQ
                     KKDMQTGFLIDDSQMNKLSNEESQKTYNEMVRQNRIDQNAAKADRDKAEKAQQENKAQ
                     IASLASQVQQLTTQLTEMQTSRNGNRNLDAGGPRKNVNEQAPAKPYQLNPNAAVNGVS
                     SGYAPISPTRNSPMRTITQSSIKTNGTDGVIQVMPISENRIREGREVVAGSEKAPTRT
                     IRGDGTAPVDSKARHAARKDEMFLPATSIITGVLITGLEAPTSLSSKAEPMPVTMRIK
                     KDIIMPNNYTMDLRDCNLLGSAVGDLASQRAYIRATSISCVNSKGKAFDVIVEAYAVS
                     ENDGKNGIRGNLISRNGNAIAGSAFAGGLSALAGSLSPSKVSSLNIDPNSTAQYQSPN
                     IGALGALAGAGAAQGGLNRLVDYYTSIAEQQWPIVEISPGRPITFVVQKGATIPTNLT
                     SR"
     gene            5507..6019
                     /locus_tag="L2X49_003976"
     CDS             5507..6019
                     /locus_tag="L2X49_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN4798983.1"
                     /translation="MENNNDNESPKKSESADSLNIDAAKPVGGIRTTKKVTLEIDIEK
                     TLKYLMVAGLAVLIVIYGYKGGRFVYDNFKAMSQPAYQIAVLDMQTLRKVFNKQNPVL
                     DSQQSKTNFENYFKALMKVYRERGYLVIDASLAVTIPENVEIVSYIDLGGSFGEVQSL
                     PGEPKESEIR"
     gene            6125..6877
                     /gene="htdT"
                     /locus_tag="L2X49_003977"
     CDS             6125..6877
                     /gene="htdT"
                     /locus_tag="L2X49_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide isomerase HtdT"
                     /protein_id="HBN4798984.1"
                     /translation="MEKAKEGGVSTKPLDGVLAKMKSYKPKDAIYIPTGGLYLFQDER
                     SQLMAVTTDGRYTITGGSVMDILQRKSVLSVEDIRKSFFINLDDAPFPLETVASIPLG
                     NPKLKRQAAIFITLDCDGCQDLIKKFYDEREKYRVDIVLIPSPGEPKQELRQLWCSKE
                     KGKVNDLDILRWLMGNKADIEKRLLTKEEAEACPAEPLVASLMLAGIYKLQGVPSVVR
                     QDGLAGNGIPKDFDYWLKQSVEPLLKNPFDTN"
     gene            6887..7837
                     /locus_tag="L2X49_003978"
     CDS             6887..7837
                     /locus_tag="L2X49_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HBN4798985.1"
                     /translation="MNIKKVIFSALVVGSSSYLAGCTIGSAESECPGIEKGVICKGPR
                     EVMELTNNRDDLSGLGEEEANSGKGKSAVNDSQYPAQLTPPGAVQYPKSSVLVNKPVT
                     YTTTEVKPVGQLPVMYDQTLKMGAPTSTIGPRPISGNPVNSNVRMTTSYNSNGSSVNP
                     FLHPSAEVVKKTTQVVTPAPAPRYVAPNSDINASKDLYSINNGQPVNPTLSSGQVQQY
                     RTQGYKQAVVAPEPLAVLQQGRVMRITFAPYTDDNDALNLPGFVYVNVKPQTWIAGKN
                     STSNPARIVPLEVQDAARENMQQQQRATKAVSSNGIVRQL"
     gene            7846..10527
                     /gene="trhC"
                     /locus_tag="L2X49_003979"
     CDS             7846..10527
                     /gene="trhC"
                     /locus_tag="L2X49_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer ATPase TrhC"
                     /protein_id="HBN4798986.1"
                     /translation="MRSANVYNKELSRHQLGGFIPVYDQIPGTHYFLLDGNRLGFMFI
                     CSPSPGVFDNQQDVLTELFKMDFPTDTICQTSLTALPDLILHLSAWSAVRGGRMEGHD
                     KLKGDLLTAYQLDYYDRSLNEPLKPDHDKLMLRDFQVWISFSIPLKSALPSEIEKTRI
                     DALYSDLISKLNTVGLFPHKVGAENWLYCMDKLLHPGKTSRWSEGHVEASTMRRLNEQ
                     INVPGRKYTVTENHFSSTTQSNDISEHRYFKQLSVVKFPEFVNFGCMYELVVNWLNGR
                     KTIFSPFMITQTVHFADPLKLSRENVRYKAITNKQASIPTVLTFCPRLKDMDNDYMTI
                     TRELEDGARLLHSYLTFTVMGNSAVDVQSAADQLKSFYLESRVNVADDSYIVFPSFVS
                     SLPMCNDPKTILELDRFEVVSNTGAAHMTPIFGPWKGNTDRPVLNLVSREGQLLGLDI
                     FKTSASYNMVVGATSGAGKSFWVAYIINNYLGAGPRSNNLIHYRDTFEGFKNNSYDAF
                     DPDGAQIFVVDVGRSYQGISEQYTNSQFIDFGKKPDFTLNPFAFLTDVTVGERVFDEA
                     PVFNDDSNNHDDDKDKVAQTIMVLNQLKIMASEKGNIDDFQQSVMLQLISEEYNESRK
                     VGRTGSITGFARRCSNHEDKRIKDIGDQLGQWCEGGIYGNRFTENLPPINFDSRFIVL
                     ELEELKGTPHLQTVVLMSIIQAAQHAMFIKKDGRRRLFILDEAWEYIRPDNASGSSNH
                     SNQFFSSFLEAAWRRFRKTNCAGICITQSFEDYFTSSVGRALTANSPWKIIMKQEKES
                     IEAMKVNNYFSTTDAEYERMKNIRTVKRAFSEMLVRFENFQEICRLYVDRKMELCFTT
                     DSTDRGKLWEIQSRLDCSYGEAIEILYEQEVASKSAA"
BASE COUNT         3281 a         2137 c         2518 g         2928 t
ORIGIN      
        1 aatggagagc aaaaaagtgc cttttgtata aaagcatttg ctccaatggc ggcagaaaag
       61 tgccttttgt ataaaagcat ttgcttcaat ggcggcagaa aagtgccttt tgaaaaaagc
      121 atttgtatga atgctgcggt gaaagttgct tttggtcggg aagcagggct cgcaagggac
      181 agtgtaaggt gcctttgttc gaaaagcatt tgcacgaata ggagaatgca agttgccttt
      241 ggtcggaaag catttgaatg aatggggaag tgtaagttgc ctttggttgg aaagcatttg
      301 catgaatgag tccctgatag gtgtctttgt ttgaaaagca tttgtatgaa tggacccata
      361 aaaggagctt ttgggcaaaa ggtagttggg tgaatcggag ctgggaaagt gcgtttggcc
      421 agaaagcatt tgcatgaatg gattcagaag aagtgacttt tacgataagc atttgaatga
      481 atgggcaccg cagacgcgca ttcaccaatc agcatttgaa tgaatgagct ccgcagtttt
      541 gctttcatcg aacagcattc gtatgaatgt gctccgcaga ggtggctttc attgaaaagc
      601 atttgtatga atgggggata cataatcctt gtgggtgaaa agcactcgca tcaatggggg
      661 aaaagtgact tttggaatgt catccttacc gtgtcctttg ttaaatatct ggctggatga
      721 atgccataaa aatggtaaag gggcagacga tgcccctctt ccctatttgg catgttaatt
      781 aaacgcacct tctttaatcc gattacatgc gggattcttt tctgacattc gatggctaaa
      841 aaaacaccaa gcgcaattaa gtcaaaaaaa aggtggttaa cagaaaaatg aaagaaaaat
      901 aaaaagtatt atgccaatgc gttttcgtga attcaaatca acacggagta attatggaac
      961 tgacattgaa tactaacgtt gaaagaggaa aaactaacgc ttttgcattc ttgaagtcca
     1021 aacatgcaaa agtgcctttg tttattctct tggttttagc aaattgcagt tttgcgtacg
     1081 cgggttccga tgatggagcc ttcggcgata tctgggccta catgagcgaa gccttgactg
     1141 gtgcgccggg taaaatcatc gcgtgcggca tgttgttctc ggtggcttat ttcggcgttg
     1201 taaaacctaa ccttggtttg gcgctggtat cagcattaat gatgctggta atggcaaacg
     1261 gtgaaaaaat tatcagctcg ttcctggatg ctggtattcc tctgtgattt gttagaacaa
     1321 taacaatgag ggggatattc cccctctctg gagtttttat gacaagccag tacgagatac
     1381 cgccacatac atatcgcttt ccatacagaa taaatatgcc gttactaatt ctattctggg
     1441 atgcaaagca attgggtata acattcgtga ctatcgcgag cggtaatatt tttgatttct
     1501 ttataacttc ggtagttgtg gcggtggtgt tctggtttgc atataagaaa gcagcagagg
     1561 aagggattag aggaaaacta aaacacaaac tgtggtggta cggctttttt cctggaaaat
     1621 cggtgtttag tagtcgttac tttaccgatc catttatcag aaacctctat tcttgatgga
     1681 gctaagaatg aaacttctca gcaggttaaa aattccattc attaaaaatt ccagtaatgg
     1741 cgattttgat aacaaggatg agacttcgca aaaaggaggt gaagaagtaa aaggaagttt
     1801 tctggattca aaagcacgct ttagtaaaga aattgaaggt tctgaaattg gaattacata
     1861 tagtgcattg attaaacgcg acgaaaaact cctacgtgtg aatacagttg caatgatcat
     1921 tattggggtt ctggttgtaa aaaatcaatt tctcactgat cctgtgacta tagtgctacc
     1981 gccaaatatg acggaagaag tcaaagttgt tggaaataaa gcctcagaat catataaaac
     2041 gcagtgggcc ttattcttta gtactttgat tggaaacata aacccaacaa acatcggttt
     2101 tgttacaaca actatattgg atgcgctttc tccggattta caggcaaaga cccgggaatc
     2161 actacaacag caaaccaata taatgcaggc tcgtggtgta gagcagtcat ttaagccgat
     2221 cgatatgtat tacgacacca aaaatgacat ggtctatgtc tggggaacaa agtcaactcg
     2281 tttgattaat gttccggaca aaactgaatc atccaaatgg acgtttgaat gggtcctggg
     2341 aatgaaaaat ggacgaccgc gcatagccta tgtgaaccag tattcaggta caccaaatat
     2401 taagaaaatt acaatcaacg gcaaagaaca actggcaaca ctcgataatc cgccgccgtc
     2461 aataggtaac taattatgac ttcaaaaaag gtttttcact gcaccgcgat tacagcagtg
     2521 attttgctgg caacttcgaa tgtggtattt gctgaagact atcagttacc ggcgacagta
     2581 aataatccgg tagttatgcc tgtcggggcc gatgaatttc agaacggtgt gaaaaatgcg
     2641 ataattaaag acactggcac tacgactgag accgtacaag caacaaaacc gacaactgct
     2701 ctgccaagtc tttcacctgc aagcagtgct tctcccgcag tcaatgaaat aaccggggcg
     2761 ttgtcaaata accctactct agctggttac cagcaacagg ttaaatcagg aaattttgat
     2821 agttacggaa gaccagcagg gaaagaacct caacaaaatg cacaaagtac cgggtcccct
     2881 tcaaaggcag atgaacttta tgttgaagca cgtaatcgct ataaagaagt acaacgagta
     2941 aacgttccgc ctggtggcaa tattgtactc ccggtttctc gggggctgca aaacagaatt
     3001 tcgacttcat ttaaaaatgc atctgtaagt acttcaacgc cagctgatga agccagtatt
     3061 ttcgttaatg gcggggatgt ttttatttca acaaatacag ataagcctat aggaattatg
     3121 ctttcagaag attcagttcc tgaatctact tataacctga cactggttcc actggatgta
     3181 ccaggggcca tgatttcagt aacaacttct ttgagcccga caatgcaggc aaaacgagaa
     3241 acttctcttg ataaacaaaa ttatgatgag atgctggcac gctcacaatc agaagagttg
     3301 actccttcag accctcggca ggatgaccat aaacagcgga tcattgattt gttgactccg
     3361 gttgcactgg gtgaggttcc ttctggtttc agtttacaag aagaccgctt gtcacgcata
     3421 ccaagctcag agcagtcccc atgtaatttc aatatgtacg ccaagttagg tcaaagactg
     3481 gtgggatctc gggaattgat agatgtcgtt cttgttaaga atgataagcc ttacggacag
     3541 gtagtggctg atcagcaatg tataactgaa ggcgtggttg caagcgctct attcgataaa
     3601 gcattccttc agcccgggga agagaccgaa ctttatattg tgcgggataa attatttaaa
     3661 gagcgtcaga ctcgtgttac tacgcgccca agcctgatta aaagataata tgagaaaagc
     3721 tacttttctt acagtgatct cgtttggttt agttgtcttt ttgggtacca gttattacta
     3781 ccaaactaag tatatcccga aaggcaccgt gaatatattt cctgatgtcc accgacaggg
     3841 tgagatcgtt gatgatgcct ttgggaaaag cacgattgtt ataagcgacc caacgcttgc
     3901 ggataaattt gttggaagtt caaccgagtc tgtcggtcgg catgaagtta atgtaataaa
     3961 agaattgggc acgcaaaata atggttcgaa atttgttcat gatgagtctg ataaatcaag
     4021 tttgacggtt ggagataaat ggcctagggc tggtgaacct tatatcgtgc ctcagatgac
     4081 agaatacgag cggaatttga aagttaagcg attccagcaa ccaaagaatg gagttaataa
     4141 tggacattaa aaaggcctgg gaaaataaaa ctgtcagaat ctcagtaatt ggtgctgcat
     4201 tgatggtatt gattgttgtc atcagtcaat caatctttac cacaccagtt aaaaaagaga
     4261 agaaaaccca gaaaaaagat atgcaaactg gattccttat tgatgattca caaatgaaca
     4321 agctgagtaa tgaggaaagc cagaagactt ataacgaaat ggtaaggcag aaccgtattg
     4381 accagaacgc tgccaaggct gaccgtgaca aagctgaaaa ggctcagcaa gaaaacaaag
     4441 cacagatagc cagtcttgct tcgcaagttc agcaactcac tacgcagttg actgaaatgc
     4501 agacctcaag aaatggtaat cgcaatctcg atgcaggcgg accacgcaaa aacgtcaatg
     4561 aacaagcccc agcaaagcca taccagctta atccaaatgc agcggtgaac ggggttagtt
     4621 ctggatacgc acctatatct ccgacccgaa acagtccaat gcggacaata acacagagtt
     4681 caattaagac aaacggcact gatggtgtca ttcaggtgat gccgatatca gagaaccgga
     4741 tcagagaggg gcgcgaggtt gttgcgggaa gtgaaaaggc cccaacgcga actattcgcg
     4801 gcgatgggac tgcacctgtt gatagtaaag ctcgtcatgc ggcccgtaag gatgaaatgt
     4861 ttcttccagc gacgtcaatt attactgggg tgctaattac cgggctggaa gctccaacga
     4921 gtttgtcctc taaagcagaa ccgatgccgg ttaccatgcg cattaaaaaa gacatcatca
     4981 tgcctaacaa ttacacgatg gatttgcgtg attgtaattt attgggttct gctgtggggg
     5041 atctggcatc tcagcgtgcg tacattaggg caacctccat atcgtgtgtc aactcgaaag
     5101 gaaaagcatt cgacgtaata gtggaagctt atgcggttag tgaaaatgac gggaaaaatg
     5161 gcattcgtgg gaatctgata agcagaaatg gaaatgcgat tgcaggttct gcgtttgcag
     5221 gagggctgtc agcattggcg ggtagtctga gtccatcaaa ggtgtcttcg cttaatatag
     5281 atccaaactc tacggcacaa taccaatcgc caaacatagg tgcattagga gcgctggccg
     5341 gagccggtgc agctcagggg ggtctaaacc gtcttgtgga ctactatacg tcaatagctg
     5401 agcagcagtg gccaattgta gaaattagcc cagggcgccc aattactttt gtcgttcaaa
     5461 aaggggcgac aatacctaca aacctgacaa gccgctgaga ataattatgg aaaataacaa
     5521 cgacaatgaa tcaccaaaaa agagtgagtc tgctgatagc ttaaatatcg acgcggctaa
     5581 accggttggg ggtatacgta ctaccaaaaa agtaacttta gagatcgata tcgagaaaac
     5641 tcttaagtat ctgatggtcg caggcctggc agttcttatc gtgatctatg gatacaaggg
     5701 tggacgtttt gtatatgaca acttcaaagc aatgtcgcaa ccggcctacc agattgccgt
     5761 gctggatatg caaactttaa gaaaagtatt taataagcaa aatccggtgt tggattcgca
     5821 gcaatccaaa actaactttg aaaattattt taaggcatta atgaaggttt accgtgaacg
     5881 tggctacttg gttattgatg catccttggc agtaactatt ccagagaatg ttgaaatagt
     5941 ctcttatatc gaccttggtg gaagttttgg tgaggtacaa tctcttccag gcgaacctaa
     6001 agaaagtgag attcgataat tgaaaaagtt acttttgtct ctgctgattt ccattacctc
     6061 agcgtgcccg ccgcttactc tggctgttga aaatgtatta aacaatcgtg atgccgctgc
     6121 gttaatggaa aaggcaaaag aaggtggagt atcaacgaaa cctttagacg gtgtattggc
     6181 gaaaatgaaa agctataagc ctaaagatgc tatttacatc cctactggcg gtctttattt
     6241 gttccaggat gaacgttctc agttgatggc ggtgaccact gacggacggt atacaatcac
     6301 gggggggagc gtcatggaca ttcttcagag aaagtcagtg ttatcagtag aggatatacg
     6361 aaagtcgttc tttattaatt tagacgacgc ccctttccct cttgaaactg tggcatcgat
     6421 acctttagga aacccaaaac tcaaacgtca agcggcaata tttattacgc ttgattgtga
     6481 cggttgtcag gatttaataa agaaattcta tgatgaacgc gaaaagtatc gggtcgatat
     6541 agttttaatc ccctcccccg gcgagccaaa gcaggaattg cgtcaattat ggtgcagtaa
     6601 agaaaaaggc aaagtgaatg atcttgatat cttacgttgg cttatgggta ataaggccga
     6661 tatagaaaaa cgactcctga cgaaagaaga ggcagaagca tgtccggctg aaccgttagt
     6721 cgcgtcatta atgctggctg gcatttataa attacaggga gtcccctcag ttgtaagaca
     6781 ggatggactc gctggtaatg gcataccgaa agattttgat tactggttaa aacaaagtgt
     6841 cgaaccctta ctcaaaaacc catttgatac taattaaggt aatgaaatga acattaaaaa
     6901 agttattttc agcgctctgg ttgttggttc atcttcatac ctggcgggtt gtactattgg
     6961 cagcgcagaa tctgaatgcc cgggaattga aaaaggagtt atttgtaaag gcccgcgtga
     7021 agttatggaa ctgacgaaca atcgtgatga tctctccggc cttggggagg aagaggctaa
     7081 ttcgggaaag gggaaatcgg ctgtcaatga cagccagtac cctgcccaac taacgccgcc
     7141 tggcgcagtg cagtacccca agtcgtctgt acttgttaat aagccagtga catacacaac
     7201 gacggaagta aaaccggttg gacagctccc ggtgatgtat gatcagacat taaaaatggg
     7261 ggcgccaact tcaacaattg gaccacggcc aatcagcggg aatcctgtta attctaatgt
     7321 acggatgaca acgagttaca actcaaatgg gtcatccgtt aacccctttt tgcatccgtc
     7381 tgctgaagtc gtgaaaaaaa caactcaggt tgttactccc gcgcctgcac cgcgctatgt
     7441 agcaccaaat tctgatatca atgcaagtaa agacctttat tccattaaca atggacagcc
     7501 agtaaaccct acgctcagct ctgggcaggt acagcaatac cgtacacaag gctacaaaca
     7561 ggcagtcgtt gcaccagagc ctcttgctgt gcttcagcaa ggccgcgtca tgagaatcac
     7621 ctttgcacca tatacggatg ataatgacgc gctaaatctg cctggctttg tttatgtgaa
     7681 tgtcaaaccg caaacttgga ttgcgggcaa aaattcaaca tcaaatccgg cacgaattgt
     7741 tcctttggaa gtccaggatg cggctcgtga aaatatgcaa cagcaacagc gagcaacgaa
     7801 agcagtttcg tcaaacggta ttgtgagaca actctgaggt cacgaatgag atcagctaac
     7861 gtctataata aagaattaag ccgtcatcag ttaggtggat ttattcccgt gtatgatcag
     7921 attccgggta cacattattt tctacttgac ggaaaccgac tcggttttat gtttatctgt
     7981 agtccgtcgc ccggtgtctt tgataatcag caggatgtgt taactgaatt atttaaaatg
     8041 gacttcccaa ctgacaccat ctgtcagacc tcattaactg cgctgccaga cttgatcttg
     8101 cacttaagtg cctggtctgc cgttcgtggt ggtcgaatgg aaggtcatga taagcttaag
     8161 ggggatttac tcactgcata tcaactggat tattatgacc gaagcctgaa tgaacctttg
     8221 aagccggacc acgataaatt gatgcttcga gattttcagg tctggatttc cttttcaatt
     8281 ccattgaagt cagcacttcc ttctgaaata gaaaagactc gcattgatgc gctttactct
     8341 gacttaatca gtaagttgaa tacagtaggc cttttcccac acaaagttgg tgctgaaaat
     8401 tggctttatt gcatggataa gttattgcat ccgggaaaaa catcgcgttg gtctgaaggt
     8461 catgttgaag ctagcaccat gagacgccta aacgagcaga tcaatgtacc gggtcgaaaa
     8521 tatacagtga cagagaatca tttctcatct acaacgcaga gcaatgatat ttctgagcat
     8581 cgttatttca aacaattatc agtagtgaag tttccagaat ttgtaaactt tggctgcatg
     8641 tatgaacttg tagttaattg gttgaatgga cgtaaaacga tctttagccc tttcatgata
     8701 acccagactg tccatttcgc cgatccttta aaattatcaa gagaaaacgt cagatataag
     8761 gcaattacta acaaacaagc cagtattcca acagttttga cgttttgtcc aagattaaag
     8821 gatatggata atgattacat gacgataaca cgcgaactgg aagatggggc tcgcctccta
     8881 cacagttatc tgacatttac tgtaatgggg aattcagcag ttgatgttca gtctgccgca
     8941 gaccagttaa aatcatttta tctggaaagt cgtgttaatg ttgctgatga ctcgtacata
     9001 gtatttccct catttgtttc aagcctgccg atgtgtaatg acccaaagac aatactagaa
     9061 ctggatcgtt ttgaggtcgt gagtaatact ggtgctgcgc acatgacccc catatttggt
     9121 ccttggaaag gtaatacgga cagacccgta cttaaccttg tctcacgaga aggacagctg
     9181 ttggggctgg atatcttcaa gacttctgcc agctataaca tggtcgttgg tgcaacgtct
     9241 ggtgctggta aatcattctg ggtcgcatac atcattaata attatctagg cgcaggtccg
     9301 cgttctaata atctgatcca ttatcgagat acttttgagg gattcaaaaa caattcttat
     9361 gatgcatttg accctgatgg agcccagatt ttcgtagttg atgtcggacg ctcataccaa
     9421 ggtatttcgg aacaatatac taatagtcag tttatagact ttgggaaaaa gcctgatttt
     9481 acgctaaatc cattcgcatt tttgactgat gtgactgttg gagagcgtgt ttttgatgaa
     9541 gcacctgtct ttaatgacga ttcaaataat catgatgatg ataaggataa agtagcccag
     9601 accattatgg ttctaaacca gttaaaaata atggcatctg aaaaaggaaa tattgacgat
     9661 tttcaacaat cggtcatgct gcaactgatt agtgaagaat ataacgaatc acgcaaagtt
     9721 ggacgtacag gctctattac tggatttgcg cgacgctgtt ctaaccatga agataagcgt
     9781 attaaagata tcggtgacca gttggggcaa tggtgtgaag gagggattta tgggaatcgc
     9841 tttacagaaa atctcccgcc aataaatttt gatagccggt ttattgttct ggaacttgaa
     9901 gaattgaagg ggaccccaca ccttcagact gtcgttctga tgtcgattat tcaggccgct
     9961 cagcatgcaa tgtttatcaa gaaagacgga cgccgccggt tgttcatact tgatgaggca
    10021 tgggagtata tccgtccgga caatgcttca ggttccagta atcattcaaa tcaatttttc
    10081 tcctctttcc ttgaggccgc ttggcgccga ttcaggaaaa cgaactgtgc cggtatttgt
    10141 attactcaat cctttgaaga ttacttcacc tcatcggttg gtcgcgcact aacggctaac
    10201 tcgccgtgga agatcatcat gaagcaggaa aaggaaagta ttgaagccat gaaggtgaac
    10261 aactacttct ccacaactga tgcggaatat gagcgtatga aaaatattcg aacggtaaag
    10321 cgtgcatttt ccgagatgct cgtacgattc gaaaatttcc aggagatatg tcgcctctat
    10381 gtagatagaa aaatggagct ttgctttacg actgacagca ccgatcgtgg aaaactatgg
    10441 gaaatccaaa gccgacttga ttgctcttac ggcgaagcaa ttgagattct gtacgaacag
    10501 gaagttgcga gcaaatcagc tgcctgacgt tttaagttac tggttacctc attttgtttt
    10561 gactgaggta tccagtacgt ctttgaagta aatagttgtt agctgtcatt gcctgccctc
    10621 ccccgaaata atagcccggg ttaagaatat ctagctcgga ttaagtcctc tcaaaccgtc
    10681 atacatgaaa ttcgatgcct ttgtcattct tcttttgaag gtgaatgaat atgatgaaat
    10741 ttactccaga attattctcc ctaccaggct tttgctgtga agttaatcag atcaggtatt
    10801 atgttttggg tacaagaacc aaccaagggg gggtgtggtt tctgtatggc agatacaaac
    10861 atca
//
LOCUS       DAFNBJ010000159        10841 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000159 DAFNBJ010000000
VERSION     DAFNBJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.159"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            238..1011
                     /locus_tag="L2X49_003980"
     CDS             238..1011
                     /locus_tag="L2X49_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4798987.1"
                     /translation="MIYSNQIKYSGKDGIYFHLRDNVDAFLTLSATENMEFDSPMQVT
                     TQNMQSGQTVTDNVQRAPRTITISGVVVVGYEGSLLLTRQGQLVENFIDTLESWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATG
                     RTSTTSDGGATSKKNTGNTTTSLGNGMLLCQELYNRSTSELSNEQLQARINCGRSVKV
                     ENGKSTFSDAANEQASKALNNAGTGNVLKRLSVNPNKRY"
     gene            complement(1026..2483)
                     /locus_tag="L2X49_003981"
                     /pseudo
     CDS             complement(1026..2483)
                     /locus_tag="L2X49_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2571..3317)
                     /gene="istB"
                     /locus_tag="L2X49_003982"
     CDS             complement(2571..3317)
                     /gene="istB"
                     /locus_tag="L2X49_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HBN4798988.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTSLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
     gene            complement(3332..4873)
                     /gene="istA"
                     /locus_tag="L2X49_003983"
     CDS             complement(3332..4873)
                     /gene="istA"
                     /locus_tag="L2X49_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HBN4798989.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            complement(5189..5683)
                     /locus_tag="L2X49_003984"
     CDS             complement(5189..5683)
                     /locus_tag="L2X49_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBN4798990.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIF
                     IRHV"
     gene            complement(5680..6246)
                     /locus_tag="L2X49_003985"
     CDS             complement(5680..6246)
                     /locus_tag="L2X49_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBN4798991.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARLRSVEKILHAL"
     gene            6556..8328
                     /gene="aspS"
                     /locus_tag="L2X49_003986"
     CDS             6556..8328
                     /gene="aspS"
                     /locus_tag="L2X49_003986"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBN4798992.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            8446..8898
                     /gene="nudB"
                     /locus_tag="L2X49_003987"
     CDS             8446..8898
                     /gene="nudB"
                     /locus_tag="L2X49_003987"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBN4798993.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            8927..9667
                     /locus_tag="L2X49_003988"
     CDS             8927..9667
                     /locus_tag="L2X49_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBN4798994.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            9702..10223
                     /gene="ruvC"
                     /locus_tag="L2X49_003989"
     CDS             9702..10223
                     /gene="ruvC"
                     /locus_tag="L2X49_003989"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBN4798995.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(10225..10827)
                     /locus_tag="L2X49_003990"
     CDS             complement(10225..10827)
                     /locus_tag="L2X49_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBN4798996.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         2823 a         2557 c         2656 g         2805 t
ORIGIN      
        1 ttatgacctt tagcagtaat atctacgacc aatacgcaga agctacgcta ttaagttcat
       61 cattcccaga agatgattga taatagtcga atgctacgtg aaatcttctg aacagatata
      121 cccaccaaaa attatttttt acgtagctca cgcttaacag agaaaacact tatatatcaa
      181 aggcttggtt aaaaatgatt aaaatacact aagtctttga aatcaaagga gaatttaatg
      241 atttactcta accaaatcaa atatagtggc aaagatggca tctatttcca cttaagagat
      301 aatgtagatg ccttcttaac cttatcagca actgaaaaca tggaatttga cagccctatg
      361 caggtaacta cacagaacat gcaatcaggg caaaccgtca cagataatgt gcaaagagca
      421 cccagaacaa tcactattag tggtgtcgtt gtagttggct atgaaggaag tttattatta
      481 actcgtcagg gtcaattagt agaaaacttc attgacactc tcgaaagctg gcgtgaccag
      541 aagcaaatta tttcggtcat ctgtaaagac ggtatcaaga ttgatgactc cattatcacg
      601 agttttaaag cctctaaaga tgttggaatt tcaaacggct taagaatcca attaactttt
      661 caggaaatta actttaaagc tattgtgggt cagactgata tctcggctgc gaccggaagg
      721 acttctacca caagtgatgg tggtgctacc agtaaaaaga atactggtaa tactacaaca
      781 agtctaggta atggtatgtt gctgtgtcag gagctatata atcgctctac gagtgaactt
      841 tctaatgagc aacttcaagc ccgtatcaat tgtggtagaa gtgtcaaagt tgagaatgga
      901 aaaagcacct tctcagatgc cgctaatgag caagcttcaa aggcgcttaa taatgctggt
      961 acaggtaatg ttttgaagag gctttctgtg aatcctaaca agcgctactg acgatagaat
     1021 ctcaactata tgataacctc atcaacatct attaaacccc tctcagtgac ttcagaaagc
     1081 gactgtatag cctctgtaac gtcctctgag gggttttctg ttattggtaa ggtgattgct
     1141 ctaccgttgt gaaaagttac tttacagcga cgtttagcag tatttaaaac gacctcttta
     1201 acagcacttt tgattatcag ttgtgcttca atccttcccg cctttgtagt caagtcacaa
     1261 tcagacaaag agcctgtatc gatagactta agccgctgtg tctccttctc gatatccgca
     1321 tcaacctgtg atagctcacc ttttaactgt tttagtttgc tggtaatagc ggacaagtct
     1381 tcaatttcaa gtgatagctg taccagcttt tcaatattgt ttgagatgtg ttcacggtgt
     1441 agcttcagtg tgtctatggt attcgtcgta tcgctatcaa atcggtcaag acgagatagc
     1501 agggtaagta atgtaccatc ggtttgctta cgtgaaagtc ctttagcact acaacggttt
     1561 tcattacgaa ggggacaacg ataaatcccg tttcttgtat tgctaaagcc tgtgataatc
     1621 agagcatgac cacattcaga acaacggata acacctttaa agagattcac actcaatggt
     1681 ttatctgaag caggttttcg accgtaacga gaatgtgata gttgctggac tttgtagaat
     1741 gttgtctcat caattacaga cgggtaataa cctgcaatct cttcacagcc tttcggggta
     1801 aagtatccaa ttacagagcg attcttcaaa aggtcttgaa cagatgtttg accaaactta
     1861 cccggcttgt attggttcat cgaccggtga ccttcagcat taagagttct gacaatctcc
     1921 cagatagact taccctctaa ccgtagctga aagcacctct tcacagtctc cactttctca
     1981 ggtatcacag agaagctttt acggtcatca ttaagcttaa gccatgcagg acaacgttta
     2041 gaaactttga taccagcttt agcagcttct ttcttggcat cccatgcagc ctgtatacgc
     2101 tcagccttac gtaatgattc ctcatgcgcc ctttgcatta tcaaaatgct tttaatcagg
     2161 gataacgggt ctcttaagct ttcctttgtg taatgttcac catcggacag ggtgactaca
     2221 tctacaccag agcgaagaat gtttcgtaat agctcacttg ctcggtctac ggcctgacgt
     2281 gacaaacggt ctaaactctc gattagtagt gtatcacctg cttttacaag ccctttctcg
     2341 atagcggtta agaagtcacc taaaccacct ttcaagtgct taccagtgaa tccactcaca
     2401 cctaaatcac gaaaagataa atcttggtca aggtagtact caggatgagc cttaagccaa
     2461 tcagtgacca tcttagattg tctgctgcgt attaccgtga aggagatcgg tgagtaacat
     2521 cgatggagat cggttcgtgt cactttcaca gaaccgtttt taaattacct tcactgatct
     2581 ccttcggtca acggagattg tattttccgc attgattctc ctttaagttc gatcttgata
     2641 ctgccatgaa ccagtcgatc taggatggca tccgcatgtg tggagtctcc gatcattttg
     2701 taccagtttt ccaccggcaa ctggctcact acgatgattg agcctcgttg gtacatcaga
     2761 tccactattt ccagcaggtc gctacgctgt tctgatgaga gaggttccag cccccagtca
     2821 tccagaagca gcagatcgct attattcaga ctggtcaaaa gtttgctgta tcttccatca
     2881 gcatgcccct gatagcactg ttccatcagc gctttaaggc gataatagta gaccttgtat
     2941 ccctgtcggc aggcattatg accaagtgca catgccagga acgttttacc gctgccggtg
     3001 gccccggtca gtaaaatatt ttgtttcagg gttaaccagt ttccctgact gagtgaacgg
     3061 atgagggccc tgtccagccc tctattgtta cgataatcca gctttgataa ctcagcatta
     3121 agtctgaacc gtgcatgttt gatcagacgc tctgccttcc tgttttcacg gcaggttagt
     3181 tcttctgctg tcagtaatga caggcgttct tcgaagccca gctcctggta tgtgcccggc
     3241 tgagcaagtt gctttttaag cgcatcacgg aagccggtga gtttaagtgc ggtcagttgt
     3301 tcgtaaagat gattcatcat tggatcccgt atcagtggta atactcactg ccgcgtacgt
     3361 tttcgtgttc catcgtggat aacagatctg gttttggatc ctgaacaggt tgtttatcca
     3421 gacctttttc caggatcgat ttaatacctg acagacgcca tacctttgtt ttcagagctc
     3481 ttgcacatgc tgcattaagt ctggctttac tgtatttttt atgaaggttc aggagtccaa
     3541 ggcagaagcg atagctttgt tccggatgtg gacgagagtt cagtatatga agcacataac
     3601 tatgagtttc actgcctatg tgccccgccc attccagaag acgctctggc gtccaggtgg
     3661 catgctgtcg atgagcttca ggcatgtgct cgttgcgggt actgtagcca taagtacgct
     3721 tgcgcgggtg cacagcaacc tcctgcccct gattgaagag tcttaccagt tctccggaga
     3781 tccatgcttc cagttgctgg cctaacagcg aacatggaac cgagtagtaa tgtttgtcga
     3841 tttccacgtg gtaatcggca tgaactctga ctttcttcac cagggtgtaa ctgtaactgg
     3901 cttcaggaag aggcttcagt gcgggtttat caagctggat gaagagttct gcacgtgaat
     3961 aacccaactt ctgcattatt ttgttattca gtctttccag caactcccga atgcgctgat
     4021 taagcgatgc aaggctgtag aagatctcat gcctgattcg ggccatgatc cagcgttcaa
     4081 caacctgaac gccaacttca gctttggctt tatctttcgg tttacgtggc cgcgcaggca
     4141 aaactgcgac attataatgc tcaagcatct gctggtaggt agggttaacg tcaggatcat
     4201 acttacatgc cctggatgtg gcgcttttca gattgtccgg aacaacaagt tcaggaacgc
     4261 cacccaacca ctggaagcag cgaacatgac tcatcaccca gtcttcaagc tgctgagacc
     4321 aggtggcctc tgcccatgtg taacttgatg ccccgagaac agctacgatg acctgagcag
     4381 ttcttatttc tccggtctca gggtcggtaa cgccaacggt aggtccacag taatcaacga
     4441 aaagtttttc gccagcttta tgtacctgac gcattgatgg tgaagtggtt ttgagccatt
     4501 cacggtacat ccggcagtaa tggttatagc tgtaaaaacc gcctggatta cgctcacagt
     4561 attcttccca gagtagctgc agcgtcacgc atttattacg cagttcccgg tgtactgtag
     4621 cccagtcagg cagagagtgc ttcttcatct taacctgggt ctgaaggaac gcatgtttta
     4681 gttttgtatc atcccatcct gtaggtaagg gccactgctt tatgccaagt tgagccgccc
     4741 gattagcata tctggataca acggaagggg agattgcaag actacgacca atttgtcgat
     4801 ggctgagtcc aacaccgtat ttaagcctaa gaatttcttt aagttttctc atagaaattg
     4861 gaactgttgg cataggtatc ctttaccgga atggcaaaag atacagatca acacacctgt
     4921 gaagttccaa taacattgat ggagatcact gaataacaaa atgagtcaaa agtgatctcc
     4981 atcgatgtta ttcagcgatc tgttcaaatg ttattacccg atctcgatgg aagttattga
     5041 gtgatctcct ttcatgaaaa tacgcagtct gcggagtgag tcgcctttag cttgcttctc
     5101 tgatgagaaa cgaatgtatg aaatgacacg tttcattgtt taccacctca aaactgatac
     5161 ctagattgta tcgctttagg tagtgtattt aaacgtgtct aataaaaatc acccgattaa
     5221 cgccgcgctg atgtaaaccg cgattaagcg tttgttcctc ttcccctttg gcgaaaaact
     5281 gtggatggcg gacttccaca ccataattaa attcgccagg gagagaatcg agaaaatgcc
     5341 aaagcgcagg cagctcccgt gggccgaatg tggcaggcag ttgcagccag tattgcccaa
     5401 tgcgcggagc caacggtgac atgcgggtca aaaattcagt cactaaatca tcgcaatgcc
     5461 gtaatgctgc ctgatgcgaa atggtcgccg gaaacttaaa acagaagcgg aagtcatctg
     5521 tggtctgttc acgccagcgc aggacaacct cgggtttcgg cagggcgtaa agcgtggtgt
     5581 tgccctccac gcagttaaag tggcgggcat actcttcaag gctggtgatc cccaaccgca
     5641 cccatttagg atgcgaccat tgcggtagac cgatgtaaat cataacgcgt gaaggatctt
     5701 ttccacgcta cgcaaacggg cgatgcgcgg gtagatatga ttaatgctgt tattgtgttg
     5761 ctcggcgctg gcggcactac aggcgtcttc ggcaatcacc agattaaagc caagttccca
     5821 ggcattgcgg gcggtggatt caacaccgat attggtcgag atcccacata acactattgt
     5881 atcgatacct cggcggcgca gttgcaactc cagatccgta ccgtaaaacg caccccactg
     5941 acgtttgatg atttcgatat cactgtcagt tgcacctaat gcagcaggat gttgccacca
     6001 gttttcgggt aacactttag ccggtgaggg cgcatcgacc ggctgtttta atgcttcggc
     6061 gtaatcggca gaccagccaa cgcgcaccag aaacacgggc tgaccgctgg cgcgaaattt
     6121 cgccgccagc ttcccggcgc gattaactac ctcatcggca gtatgtggac caccggcaaa
     6181 aggtaagatg ccttcttgta aatcgatcac caccagcgca gtggttttag cattaagttc
     6241 aagcataatg actcctgaaa aaacaaaatc gtgcctcaca ccttaatgcg attagccgat
     6301 ggcggaacga caaattttgt taatttttat gagttcgggc aacaccttta tagcaaactg
     6361 gcgtagagtc tgatttcagg cccgatataa agtggtaacg aggcgttcca gcgcgggcca
     6421 ggctcttacg gtgcggcaga atttccagta taatagccgc cttttttcat tcagttgtga
     6481 catacagcta acgctgcgac ttggtcacct gcgcagcagg gggcatccgc cggcagctaa
     6541 gttaagggat atctcatgcg tacagaatat tgtggacagc tccgtttgtc ccacgtgggg
     6601 cagcaggtga ctctgtgtgg ttgggtcaac cgtcgtcgtg atcttggtag cctgatcttc
     6661 atcgatatgc gcgaccgcga aggtatcgtg caggtatttt tcgatccgga tcgtgcggac
     6721 gcgttaaagc tggcctctga actgcgtaat gagttctgca ttcaggtcac gggcaccgta
     6781 cgtgcgcgtg acgaaaaaaa tattaaccgc gatatggcga ccggcgaaat cgaagtgctg
     6841 gcgtcctcgc tgactatcat caaccgcgca gatgttctgc cgcttgactc taaccacgtc
     6901 aacaccgaag aagcgcgtct gaaataccgc tacctcgacc tgcgtcgtcc ggaaatggct
     6961 cagcgcctga aaacccgcgc taaaatcacc agcctggtgc gccgttttat ggatgaccac
     7021 ggcttcctcg acatcgaaac tccgatgctg accaaagcca cgccggaagg cgcgcgtgac
     7081 tacctggtgc cttctcgtgt gcacaaaggt aaattctacg cactgccgca atccccacag
     7141 ttgttcaaac agctgctgat gatgtccggt tttgaccgtt actatcagat cgttaaatgc
     7201 ttccgtgacg aagacctgcg cgctgatcgt cagcctgaat ttactcagat cgatgtggaa
     7261 acttctttca tgaccgcgcc gcaagtgcgt gaagtgatgg aagcgctggt gcgtcatttg
     7321 tggctggaag tgaagggcgt ggatctgggc gatttcccgg taatgacctt tgcggaagca
     7381 gaacgccgtt atggttctga taaaccggat ctgcgtaacc cgatggaact gaccgacgtt
     7441 gctgatctgc tgaaatctgt tgagtttgca gtatttgcag gtccggcgaa cgatccgaaa
     7501 ggtcgcgttg ccgctctgcg cgttccgggc ggcgcatcgc tgacccgtaa gcagatcgac
     7561 gaatacggta acttcgttaa aatctacggc gcgaaaggtc tggcttacat caaagttaac
     7621 gaacgcgcga aaggtctgga aggtatcaac agcccggtag cgaagttcct taatgcagaa
     7681 atcatcgaag ccatcctgga tcgtactgcc gcgcaagatg gcgatatgat tttcttcggt
     7741 gccgacaaca agaaaattgt tgccgacgcg atgggcgcac tgcgcctgaa agtgggtaaa
     7801 gaccttggtc tgaccgacga aagcaaatgg gcaccgctgt gggttatcga cttcccgatg
     7861 tttgaagacg acggtgaagg cggcctgacg gcaatgcacc atccgttcac ctcaccgaaa
     7921 gacatgactg ctgcagaact gaaagctgca ccggaaaatg cggtggcgaa cgcttacgat
     7981 atggtcatca atggttacga agtgggcggt ggttcagtac gtatccataa tggtgatatg
     8041 cagcagacgg tgtttggtat tctgggtatc aacgaagagg aacagcgcga gaaattcggc
     8101 ttcctgctcg acgctctgaa atacggtact ccgccgcacg caggtctggc attcggtctt
     8161 gaccgtctga ccatgctgct gaccggcacc gacaatatcc gtgacgttat cgccttcccg
     8221 aaaaccacgg cggcagcgtg tctgatgact gaagcaccga gctttgctaa cccgactgca
     8281 ctggctgagc tgagcattca ggttgtgaag aaggctgaga ataactgata tgactcaaat
     8341 acacgaaatc attcgcgttg catcaaggcg gcaactgagt gaactcccat gagcatagat
     8401 aactatgtga atgggatgag cgaaggcagt caacgaagag gcagcgtgga ggataaagtg
     8461 tataagcgtc ccgtttcgat cttagtggtc atctatgcac aagatacgaa acgggtgctg
     8521 atgttgcagc ggcgtgacga tcccgatttc tggcagtcgg taaccggcag cgtggaagag
     8581 ggtgaaaccg cgccgcaagc tgccatgcgc gaagtaaagg aagaggtcac cattgatgtt
     8641 gtcgctgaac aactgacctt aattgactgt cagcgcacgg tagagtttga aattttttca
     8701 catttacgtc atcgctatgc gccgggcgtg acgcgtaata cggaatcatg gttctgtctt
     8761 gcgcttccgc acgagcggca gatcgttttc actgaacatc tggcttacaa gtggcttgat
     8821 gcgcctgctg cggcggcgct cactaagtcc tggagcaacc ggcaggcgat tgaacagttt
     8881 gtaattaacg ctgcctgaac aggcagggct atctggagat atttttatgg caggtcatag
     8941 taaatgggcc aacaccagac atcgtaaagc tgcgcaggat gctaagcgcg gtaaaatctt
     9001 cactaaaatc attcgtgagc tggtaaccgc ggctaagctg ggcggtggcg atccggacgc
     9061 taacccgcgt ctgcgtgcgg cggtggataa agcactgtct aacaacatga cccgtgacac
     9121 actgaaccgc gcaattgcac gtggtgtggg cggtgatgat gatgcaaaca tggaaaccat
     9181 catctacgaa ggttacggtc ctggcggcac ggcaatcatg attgaatgtc tgtctgacaa
     9241 ccgcaaccgt accgttgctg aagtgcgtca tgcattcagc aaatgtggcg gtaacctcgg
     9301 tacagatggt tccgtagcct atctgttcag caaaaaaggc gtgatctcct tcgagaaagg
     9361 cgatgaagac accatcatgg aagcagctct ggaagcaggt gctgaagacg ttgtgaccta
     9421 tgatgacggc gcgattgatg tctacactgc atgggaagaa atgggtaaag tgcgcgacgc
     9481 tctggaagcg gcaggtctga aagcagacag cgcggaagtt tccatgatcc cgtccaccaa
     9541 agctgatatg gatgcagaaa ccgcaccgaa actgatgcgt ctgatcgata tgctggaaga
     9601 ttgcgacgac gtgcaggaag tttaccataa cggtgaaatc tctgatgagg tcgcagcgac
     9661 tctctgatga ggcctgctaa acagcaaaac ggagacgcgt gatggctatt attctcggca
     9721 ttgatccggg ttcgcgcgtg accggctatg gcgtaattcg ccaggtaggc aggcaactgt
     9781 cctacctggg tagcggatgc atacgcacca aagtggatga tttaccgtct cgtctgaagc
     9841 tcatctatgc gggcgtgacg gaaatcatca cccagttcca gcctgattat ttcgccattg
     9901 aacaagtctt tatggcgaag aacgctgact cagccctgaa actgggccag gcgcgcggcg
     9961 tggcgattgt tgcggcggtg aatcaggaat tgccggtatt tgaatacgcg gcacgtcagg
    10021 taaagcaaac ggtggtaggt attggtagtg ccgaaaaaag ccaggtgcag catatggtcc
    10081 gcaccttgct gaaactgccc gctaatccac aggcggatgc cgccgatgcg ttagctatcg
    10141 cgattaccca ttgtcacgtc agtcagaatg cgatgcagat gagcgaatcg cggctgaacc
    10201 tggcgagagg gcgactgcgt taagttatac cgcctcggtc agttcaggct gaggcgtttc
    10261 aactccctcc gcgtgaatca acaccaaacc cggatgatgc cacaaactgg ctggcgtgac
    10321 ggttttacgc tcccacggaa tagaacctaa gaaccagaat ttccagaaag tgagttttgc
    10381 attcggattg ctatttaaca gttgtccaaa cgtctctatt tcacccaccg gaatacatag
    10441 cgcctcttta taaatatcaa agacaaattt tgaacagaac tggcgggaag attcgtattt
    10501 aaaacccgtg tggtaaagtt tgcgtagccg ggaaggaacc tgttcaacaa ttcgttgttt
    10561 ttgttgttct gtcagcccag cgtctaatcg ctttatagca tagcgttgat tagacgaacg
    10621 tttaataaaa cgagatagcg tggtgatggt tgaaagggga acccggcttt ctgcaaccag
    10681 gaagtcttca ccgttatgac cgataatgat cccgacgtga ttactccagc aatttgatgc
    10741 agctgatatt tgaccaaata aggcagcacc tatacatgta aagacgatat ctccaatttc
    10801 atattcagca gggtaattaa tattcacgtt gtcattccat t
//
LOCUS       DAFNBJ010000160        10833 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000160 DAFNBJ010000000
VERSION     DAFNBJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.160"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(345..2027)
                     /gene="glcA"
                     /locus_tag="L2X49_003991"
     CDS             complement(345..2027)
                     /gene="glcA"
                     /locus_tag="L2X49_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HBN4798997.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2383..4554)
                     /gene="glcB"
                     /locus_tag="L2X49_003992"
     CDS             complement(2383..4554)
                     /gene="glcB"
                     /locus_tag="L2X49_003992"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBN4798998.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAVVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4576..4980)
                     /locus_tag="L2X49_003993"
     CDS             complement(4576..4980)
                     /locus_tag="L2X49_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HBN4798999.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(4985..6208)
                     /gene="glcF"
                     /locus_tag="L2X49_003994"
     CDS             complement(4985..6208)
                     /gene="glcF"
                     /locus_tag="L2X49_003994"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HBN4799000.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(6219..7271)
                     /gene="glcE"
                     /locus_tag="L2X49_003995"
     CDS             complement(6219..7271)
                     /gene="glcE"
                     /locus_tag="L2X49_003995"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBN4799001.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGEGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7271..8770)
                     /gene="glcD"
                     /locus_tag="L2X49_003996"
     CDS             complement(7271..8770)
                     /gene="glcD"
                     /locus_tag="L2X49_003996"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HBN4799002.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9021..9785
                     /gene="glcC"
                     /locus_tag="L2X49_003997"
     CDS             9021..9785
                     /gene="glcC"
                     /locus_tag="L2X49_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBN4799003.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            complement(9792..>10833)
                     /locus_tag="L2X49_003998"
     CDS             complement(9792..>10833)
                     /locus_tag="L2X49_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBN4799004.1"
                     /translation="NWIPPLFIERNEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQ
                     IDTLHCERYGEDTGHFGMIDAIDDPQVFAALFGAAEAWLKSQGASKISGPFSLNINQE
                     SGLLIEGFDTPPCAMMPHGKPWYAAHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLM
                     DQVRKKVTIRCINRQRFAEEMQILREIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVP
                     DDMIYIAEIDSAPCAFIVGLPNINEAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPL
                     MGVRDEYQFSRIGPVIALLLIEALRDPFARRKIDALEMSWILETNTGMNNMLERIGAE
                     PYKRYRLYEKQI"
BASE COUNT         2549 a         3042 c         2720 g         2522 t
ORIGIN      
        1 ccctatttga tgtctggctg gctattttta cgaaatttca ataaacaaaa aattaacaac
       61 atttcacaac gcaactatat aaattcgttg gtagttttaa cgattaagat tgcaaaagca
      121 acagatagaa cggtgtttgc tcatcaagcg aacataatta atacagactt gttttgcgtg
      181 ataaaaaaaa tagatgttct cacgctctta attatttagc aggttatctg aacacaaaac
      241 attattgccg catggataat aaagcgagaa ataattttca attaataaga cagaataatg
      301 taaatgaagc cggatgatat taacgatcat ccggcattat tgatttacga gaccaacatt
      361 ccagtaaaca catacgcctg caacaacgtg ataacgccga taacgctggc aaaaatcaga
      421 ctgtgcttca cggtgtagcg gaatagttca gattctcggc ccaccatgcc cgtcgcggcg
      481 caggccacgg cgatagattg cggggagatc atcttgccag ttacgccgcc gctggtattt
      541 gctgccacca gcagagtgtc agagacgttg atttgctgcg ccgtggtcga ttgcagtgaa
      601 ccaaacaggg cgtttgaaga agtgtccgag ccggtaagga atacccccag ccagccgagg
      661 aacggtgaga aaaacgggaa catcacgcct gtacctgcca gtaccagcgc caacgtggtg
      721 gacatgccgg aatagttggt gacgaaggcg aacgccagca ccatgccaat cgacaatatc
      781 ggccacttca gactaattag cgtttcggca aagacgccaa tccctttctt gatgcccacg
      841 ccgaggatga agatagagat aatcgccgca ataaaaatag cggtgccgcc agccgagaga
      901 gggtcgaatt taaacaccgc atccattggc gttggttggg cgacaatggg tgccgctttc
      961 aacacttgtt gatgcaaatg agggatctgg aaattaatca ccagtgaata aaacgcgccg
     1021 cccggagcaa ataacgcttt aaacggcttc atggtccaga tggtgaccag caccgttaag
     1081 attaaaaacg gtgaccacgc acgaatgatt tgccccagac tatattctga aggcacggga
     1141 ccgccagaag atggcttatt taccaccatc gcacctgcgg attgccccat gctgattgcc
     1201 gtttcggtat ttttcggccg ccagacttta aggaataaag cgagtgagac gatactcacc
     1261 agcgccgaag taatatccgg cagttccgga ccaatatagt tagaggtaaa gaactgagtg
     1321 acagcaaagc ttcccccagc aaccagcgcc gctggccacg tctctttcac ccctttccag
     1381 ccgtccatca ttgctaccag ccagaacggc acaagaaccg acaggaacgg taactgacgt
     1441 cccgccattg cgccaatgtg gaacggatcg attcccgtta cctgaccggc gaccagaatc
     1501 ggcacgccca acgcaccaaa cgccactggc gcagtgttgg caatcagaca cagccccgcc
     1561 gcgtataacg gtttgaagcc caggcccacc agcagcgcac cggtaatcgc caccggcgca
     1621 ccaaagccag ccgctccttc cagtaacgca ccaaaggaga aaccaatcag taacacctgc
     1681 aaacgctgat cgtcggtgat ggagataacc gagctgcgga taatatcgaa ctgaccgctg
     1741 gcaacggtta atttatagag gaacaccgcc gcgacaataa tccacgcaat aggccataat
     1801 ccataaataa agccatagcc cgcagcagca aaagccatat caatcggcat tttaaaggcg
     1861 aatattgcaa tcaggataga taatataagg gttattgctc cagcgacatg tcctttcaga
     1921 cgtaataccg cgagtgcaac gaagaagaat attatcggga tcagggcgac cagagcggat
     1981 agccccagtc ctcccatcgg catatacatt tgggtccagg taaccatatt gttcagtctc
     2041 ttattatctt tatatgcttg atatacttaa ggttgtaata agcaaaagag gactgaactg
     2101 taaaatatag gcgttatact ttacagcaac agtacgccgc taacgcaatt gctacctctg
     2161 gcataacaag tatatcgggt aagggtttct gttctgcaca cgcaggcgca gagtatcgtt
     2221 aagatgtcca tattgttgtt ataggcccgc tagtaatgcg ctacgggtat ttaatattgt
     2281 taaaccctga taatcgctcc ggttatttcc gggataaatg tactaccgca gttactatca
     2341 tagccccgac aataaaactt gccggggctt tttttgacgc tattaatgac tttctttttc
     2401 gcgtaaacgc caggcgtgta ataacggttc ggtatagccg tttggctgtt tcacgccgag
     2461 gaagattaaa tcgctggcag ctttaaaagc acacgagtta gcgaaattcc ccgccatcgg
     2521 acgataagcc ggatcgtcag cgttttgctg atcaaccact ttcgccatat tctccagcga
     2581 cgcctgcacc tgttctttgg tcagaatacc gtgacgtaac cagttggcga tatgctggct
     2641 ggagatacgc agcgttgcgc ggtcttccat cagcgccaca ttgtgaatat ccggaacttt
     2701 tgaacaacca atcccctgct ccacccagcg caccacgtac cccagaatcc cctgcacgtt
     2761 gttatccagc tcttgttgga tctcttccac cgaccagtta gcgttttcag ccaccggaat
     2821 agtcagcaga tcgtccagca gcggttcaaa ttcagcattg aactcggtct gggcaatgtt
     2881 ggcttgcacg ctctgtacgt tggtttggtg gtagtgcagc gcatggagcg tagcagcggt
     2941 tggtgacgga acccaggctg tgtttgcccc ggcacgcagt tggtcgccct tctggctgta
     3001 catgtctgcc atcaggtccg gcattgccca catgccttta ccaatttgcg ctttaccgcg
     3061 cagcccacag aacagaccgg aaagcacgtt attacgctcg taggctttga tccacggcgt
     3121 cgatttcatc tggtttttac gcagcatcgg gccagcttcc atcaccgaat gcatttcatc
     3181 gccggtacgg tcgaggaaac cggtattgat gaacgccacg cgattgcgcg cctgagcgat
     3241 acagctacgc aagttcagcg aggtccgacg ttcttcatcc ataatgccca ttttcagggt
     3301 attcggtgcc ataccgagca ttgtctcaat gcgggtaaac agtttgttgg cgaacgccac
     3361 ttcctgcgga ccgtgcattt tcggtttcac aatatagacg ctgccagtgc gcgagttttt
     3421 ctgcactttt aaatcataga gggcgatcgc gccagtcatg acgccatcaa gaatgccttc
     3481 cgggatttca ttgccttcgc tgtcccaaat cacaggaatg gtcatcaaat gacccacgtt
     3541 gcggataaac agcagcgagc gtccgtgcag agaaatttca gagccatcgg cggcggtgta
     3601 atgacgatcg tcattcagtt tacgcacgat ttgtcgaccg tttttctcca ttttctcttg
     3661 cagagtcccc tgcatcaggc ccagcagatt gcggtacagc aggattttat cttccgcatc
     3721 aaccgccgcg accgaatctt cgcagtcgag aatggtactg atagcagctt cgacgataac
     3781 atcgttgatg tgcgccggat cgtctttgcc aatccgcccg ttggcatcaa tttgcagttc
     3841 aatatgcagg ccgttatttt tcagcaaaat gcaggtcagc gcagcggcat cgccacggta
     3901 accgacaaac tgcgccgggg tacgtaacgt ggtttcttta ccatttttca actggatgcg
     3961 taattgttta tcgaccacct taaacgccac cacatcctga tagctgccgt tttccagcgg
     4021 tagagattca tcgaggaaac gccgaaccca ggcgataacc tgctcaccgc gttgcggatc
     4081 gtagccgctg accaccgccc cttcctgcgg gatgatgtcg ctgccgtata acgcatcgta
     4141 cagtgagccc cagcgagcgt tcgccgcgtt cagcgcgtag cgggcgttca ttgccggaac
     4201 caccagctgt ggccccgcct gactggtgat ttcgctgtca atgcccgtgg tttccaccgt
     4261 cacgcgctcc ggttgcggca ccaggtagcc cagttcacgc aggaaagatt tataggccgc
     4321 tttatctttt accggccccg gattgctgcg atgccactca tcaagcgccg cctgaatgcg
     4381 atcgcgttct gccagcaact gacgattttc tggtgccaga tcgtgaacga tttcatcaaa
     4441 attgcgccag aacgccgcag cgtccagccc tgttcccggt aaaacttctt catccacaaa
     4501 acgtttaaaa ttggcgtcaa tgcgtaaacg gccctgggtt atggtttgac tcattgttta
     4561 tctcctcgtt ttcgcttatt tcgccaacac cgctgctgcc gctttcgcga cctgcgcatc
     4621 ctgtgctccg gttaaaccag aaacgcccac ggcaccaata atttgcccat caacaacaac
     4681 cggtacgcca ccttccagcg acgtcagtaa cggcgcagtc acgaacgcgg tacgtccgtt
     4741 gttcaccatc tcttcatagc ccttagtttc acgacgcccc agcgcggcgg tacgcgcttt
     4801 ctcctgggag atgtaagccg caatcggcgc gcaatcgtcc atgcgactta acgccagcag
     4861 atgaccgccg tcatcggcaa cagcaatgga aacagaccag ttatttttct gcgcctcttc
     4921 ctgacctgcg gcaataattg cactcgccat ttgctggcta agaatgactt tagttttcat
     4981 ttttttattc cttttcaagg gcttgttcta caatttcaat ccagtgacgc acagaggtac
     5041 gaccggcgct cgccagatgc gtctggcaac caatgttggc ggtgacgatc atttccggtt
     5101 tgccgctttc cagcgcattc attttgttat cccgcagctg gcgtgccaga tcgggatgcg
     5161 ttaacgcata tgttcccgct gaaccgcagc acagatggct gtcgggaacg tccgttaagg
     5221 taaatccaag acgaagcaac actttttcca cttcgccgtt cagcttttgc gcatgttgta
     5281 gggtacacgg gcagtggaag gccagttttt tatcgccgcg aactgccagt ttttccagcg
     5341 gttcctcgcg cagtagctcg actaaatcga ccgccagttc actgacttga cgcgctttat
     5401 cggcatataa cgcatcgttt ttcagcatct gcccgtactc tttgacaaac gcgccgcaac
     5461 cgctggcggt ttgcaaaatt gcctcagcac ctgcttcaat cgcgggccac caggcatcaa
     5521 tattattgcg cgcccgcgcc agccctttct cctgcgcatt aagatgatag tccaccgcgc
     5581 cacaacagcc tgcttcgtta gctgacatga cgctgatccc cagacgatcc agcactcgcg
     5641 cagttgccgc gttggtgttg ggcgaaagcg taggctgggc gcagccttcc agcattaaaa
     5701 cccgacgctt atggcgcagc ggcggacgcg gtttagcttt caccgtttca gcaggcagtt
     5761 ttgctctgac ctgttccggt aaaaacggtc gcagcaccag ccctacctgc gtcagcgcac
     5821 ggaagaccgc cggacgcggc actacctggc gcaatccttc gcgcagtatt cgctccggca
     5881 gtgggcgttt tactttctgc tcgacaatat cacgcccgat atccagcaaa ttgtgatagc
     5941 gcacaccaga aggacaggtg gtttcacaat tgcggcaagt gaggcagcga tcgagatgct
     6001 cctgtgtttt aagcgtgact tcgttgcctt ccagcacctg tttaatcaga tagatgcgcc
     6061 cgcgcggccc gtccagttca tcgcccagaa gctgataggt tgggcaggtt gcggtacaaa
     6121 atccgcagtg aacacaggcg cgcaggatgc tgtcggcttc cagcgcccgc gcgttctgcc
     6181 gcatctcttc agttaattgg gtttgcatag cctgctcctc aaagttccgc gtacatgcga
     6241 ccggggttaa acacgccgca agggtcgagc tgctgtttaa gctgctggtg atagcggaat
     6301 aaaggagccg gtagcggggc aaagccaccc tctccggcac taaagcgggt cgcatgaccg
     6361 ccagcgttgc gggcgatgcg atggatttga ttgtcatcgg ctgtcgattt cagccagcgt
     6421 aacgccccgc cccagtcgat cagttgctcg ccgggtaaat ccatcatcgg cgcatcactg
     6481 ggtaatgaaa tgcgccataa ggtacctggt aacgagaaga acggcagttg ttgttcacgc
     6541 aattgctgcc agaactgacc cgcaacctct tcgcccccca gcagttcacg cgccgctttt
     6601 accgatcctt cgccgccctc aaggcggatc cacaacgcat tgtcgaagta acataagcca
     6661 ctaatgggta gcggctggag ttgccactgg gcgatttcgt tcatggcttc ttgcaggctg
     6721 atttcccgac gcaggctcag ggaggcgcgc ggtcgcggta acactttcat cgagatttca
     6781 gtgagcacgc caagacaacc gtagcttccg gccattaacc gtgagagatc gtatccggca
     6841 acgtttttca tcacttcgcc accaaaacgc agatgttttc cagcgccggt aatgatgcgc
     6901 gtgccgagga caaaatcgcg gaccgaaccg ctccacgggc gacgcggccc cgccagcccg
     6961 caggcgacca tcccgcccca ggtggcttct tcaccataat gcggcggctc gcaggggagc
     7021 atttgcccgg cgctctccag cgccgcttca atcgccacca gcggcgttcc ggcacgcgcg
     7081 gttatcacca gctcggtcgg gtcgtaatta acaatgccgc gatgacaacg cacatccagc
     7141 gtttgcccgg tgacagggcg acctaaaaag gctttgctat tgctgccctg aatcaccagc
     7201 ggcgttttat cgctaattgc ctgattcacc tgctccagca gcgcctggct gtaatcacac
     7261 tcgcgtagca tcagaaacgc tccagttcag ggaaaggtaa atgaccgtga tgcacatgca
     7321 tggcaccaaa ttcagcacag cggtgtagcg tgggaatgtt tttcccaggg ttcagcaaac
     7381 catcggggtc aaacgccgcc ttgaccgcat ggaaggtcgt gatttcatcg ctgttgaact
     7441 gggcgcacat ttgattgatt ttttctcgcc cgatgccatg ttccccactg atgctgccgc
     7501 caacttcaac gcagagttcg aggatcttcc cacccagctc ttccgcgcgg gcaaattcac
     7561 cgggttcgtt ggcatcgaaa aggattaacg ggtgcatgtt gccatctccg gcatgaaaga
     7621 cgttggcaac acgtaaatca tattgctgcg ataaacgggc aatgccttcc agtacgccag
     7681 gcagggcgcg acgcgggatg gtgccatcca tgcagtagta atccggggag atacgtccta
     7741 ccgccgggaa cgcatttttg cgaccggccc agaaacgtac gcgctctgct tcgtcctgtg
     7801 ccagacggac gtcagtcgcg cccgctttca acaagatgtc gttaacccgc tcgcagtctt
     7861 cctgtacgtc agactccacg ccgtccagct cgcataacaa aattgcttcg gcgtcgacgg
     7921 gataaccggc atgaataaaa tcttccgccg cgcggatcga caggttatcc atcatctcca
     7981 gcccgccggg gataatgcca ttggcgatga tgtcaccaac tgcaagtccg gctttttcta
     8041 ccgagtcaaa gctggctaac agaacccgcg ccacgggcgg cttcggcagc agttttaccg
     8101 tcacttcggt ggtcacgccg agcatacctt ccgatccggt gaacagcgcc agcaggtcaa
     8161 aaccaggtga atccagcgcg tccgatccca gcgtcagcgc ctcgccgtcc agcgtttgca
     8221 cttcaatttt cagcaggtta tgtacggtca gaccatattt caggcagtgg acgccgccgg
     8281 cattttctgc cacgttgccg ccaatggaac aggcgatttg tgaggaaggg tccggtgcgt
     8341 agtagagatt atgcggtgca acggcctggg agatcgccag gttacgcacg cctggctgca
     8401 cgcgcgcgcg gcgaccaacg gggttaatgt cgaggatctc tttaaagcgc gccatcacca
     8461 acaacacacc tttttccagc ggcagcgcgc caccagaaag cccggtgcct gcaccacggg
     8521 tcaccaccgg tacacgcagg cgatggcaga cagcaagaat cgctgtcacc tgttccattt
     8581 gcttaggcag aacaaccagt aatggacgcg tgcgatacgc gctcaacccg tcacactcgt
     8641 aaggaatgat ctcctcatcg gtatgcagga tctcaagtcc agggacatgc tcacgcagtg
     8701 ccatcagtac cgatgtgcgg tcgacatcgg gtaaagcgcc atcaagacgc tcttcgtaca
     8761 agatgctcat gagtaggctt cgctttgttg tgttgtgtgg cagctgattt ttgcgcgctg
     8821 cttctgtgaa cagttattaa gcgggctttt cgttttcgtc tatctcttta gctaccggtc
     8881 agaccatttt ttttccagct ctgtgacctt gccttggtta actcaatgtt aaattgatgt
     8941 aacataatca cttacgtgat gtgcgtgttt tgcgagttaa gaacagaaaa attggtccta
     9001 cctgtgcacg aggtccggga atgaaagatg aacgtcgccc tatttgcgaa gtggttgcag
     9061 agagtatcga acggttaatt atcgacggcg tactgaaggt cggtcagcca cttccctcgg
     9121 aacgtcgact gtgtgaaaag ctcggcttct cacgctccgc actgcgtgaa gggctgaccg
     9181 tgctgcgcgg gcgcgggatt attgagacgg cgcagggtcg cgattctcgt gtcgcacggc
     9241 ttaatcgggt gcaggacacc agcccgctga tccatctgtt cagtacgcag ccgcgaacgc
     9301 tgtacgatct gctcgacgtt cgcgcattac tggagggcga atcggcaagg ctggcggcaa
     9361 cgctgggaac gcaggctgat tttgttgtga taacccgctg ttatgaaaaa atgctcgccg
     9421 ccagtgagaa caacaaagag atttcgctga tcgaacatgc gcagttggat cacgctttcc
     9481 atctcgccat ttgtcaggct tctcacaatc aggtgctggt gtttacgctg caatcattga
     9541 ccgatctgat gtttaattca gtgtttgcca gcgtaaataa tctctaccat cgaccacagc
     9601 aaaaaaagca gatcgatcgc cagcatgcga ggatctacaa cgcggtgttg cagcggctgc
     9661 cgcacgtcgc ccagcgcgca gcacgcgatc atgtgcggac cgtgaaaaag aatctccacg
     9721 atatcgagct ggaaggccac catttgattc gctcggcggt gccgctggag atgaacctga
     9781 gttagctggt attaaatctg cttttcatac aatcggtaac gcttgtacgg ctccgcccca
     9841 atgcgttcca gcatgttatt catgcctgta ttggtttcga ggatccatga catctccagc
     9901 gcatcgatct tccggcgggc aaacggatcg cgtaaggctt caatcaataa cagcgcaatc
     9961 accgggccga tgcggctgaa ctgatactcg tcgcgcacgc ccatcagcgg tactcgcgca
    10021 gttcgcacac cgctgacttt caaacgccac agcaattttg cccagccgaa gggaaagagc
    10081 gatccgttca gatcggcaat cgcctcgttg atgttcggca agccgacaat aaacgcgcag
    10141 ggtgcagaat caatctcagc gatatagatc atatcgtccg gcaccagata tttaagttga
    10201 tcgcccatgg tcgcgaattc atgttcggta aacggcacaa atccccagtt gtgctgccag
    10261 ccagagttga aaatctcacg caggatctgc atctcttcgg caaaccgctg acgattgatg
    10321 cagcgaatgg tcaccttttt gcgcacctga tccatcagtt tttttagcgc cggagagaaa
    10381 gtgagatcgg ttcgctgcat ccaccacgcc agtaaatcaa tgcctttgtg ataacccagt
    10441 tgttcaatat gcgcggcata ccacggtttg ccgtgtggca tcatcgcaca gggtggtgtg
    10501 tcaaaacctt caatcagtaa tccgctttcc tgattgatat tcaggctgaa aggcccgctg
    10561 atcttacttg caccttgtga cttcaaccac gcttccgctg cgccaaacaa cgcggcaaaa
    10621 acctgcggat catcaatggc gtcaatcatg ccgaaatgac cggtatcttc accgtaacgc
    10681 tcacagtgca aggtatctat ttgcgcggta atacgcccaa ctatctgccc cgcttttttt
    10741 gccacccacg cctgccagat gatatggtcc gtccccggat ttttcgcaga caaatgctcg
    10801 ttgcgttcaa tgaataaagg gggtatccag ttt
//
LOCUS       DAFNBJ010000161        10812 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000161 DAFNBJ010000000
VERSION     DAFNBJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.161"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            55..1665
                     /gene="yeaV"
                     /locus_tag="L2X49_003999"
     CDS             55..1665
                     /gene="yeaV"
                     /locus_tag="L2X49_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HBN4799005.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            1697..2821
                     /gene="yeaW"
                     /locus_tag="L2X49_004000"
     CDS             1697..2821
                     /gene="yeaW"
                     /locus_tag="L2X49_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HBN4799006.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVQDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNCDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            2877..3842
                     /gene="yeaX"
                     /locus_tag="L2X49_004001"
     CDS             2877..3842
                     /gene="yeaX"
                     /locus_tag="L2X49_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBN4799007.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNHYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(3896..5011)
                     /gene="rnd"
                     /locus_tag="L2X49_004002"
     CDS             complement(3896..5011)
                     /gene="rnd"
                     /locus_tag="L2X49_004002"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBN4799008.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(5093..6778)
                     /gene="fadD"
                     /locus_tag="L2X49_004003"
     CDS             complement(5093..6778)
                     /gene="fadD"
                     /locus_tag="L2X49_004003"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBN4799009.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALRNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(6983..7564)
                     /gene="yeaY"
                     /locus_tag="L2X49_004004"
     CDS             complement(6983..7564)
                     /gene="yeaY"
                     /locus_tag="L2X49_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBN4799010.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(7604..8299)
                     /gene="tsaB"
                     /locus_tag="L2X49_004005"
     CDS             complement(7604..8299)
                     /gene="tsaB"
                     /locus_tag="L2X49_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBN4799011.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(8357..10267)
                     /locus_tag="L2X49_004006"
     CDS             complement(8357..10267)
                     /locus_tag="L2X49_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBN4799012.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            10399..10743
                     /locus_tag="L2X49_004007"
     CDS             10399..10743
                     /locus_tag="L2X49_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBN4799013.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         2560 a         2918 c         2722 g         2612 t
ORIGIN      
        1 ataattacaa ctggcaaaag ccagagacgg gcttttatgc ccaggagatg actcatgatg
       61 agcaatgtta agaaaaaaga tgtgccgctg ataagcatca gcctggtggc cattcttttc
      121 atcgcagctg cattaagcct tttcccacaa caatcggccg acgcggccaa cgcaatatac
      181 acttttgtta cgcgtacgtt aggttccgcc gtacaggtat tggttttgct ggcaatggga
      241 ctggtgattt atttagccac cagtaaatac ggcaatattc gtcttggcga aggaaaaccg
      301 gaatacagca cgctctcttg gctgtttatg tttatttgtg ccggtttagg ttcttctacg
      361 ctttattggg gggttgctga atgggcctat tattatcaaa cgcctggatt aaatatcgca
      421 ccgcgttcac aacaggcact cgaatttagc gtcccttact ccttcttcca ctggggcatc
      481 agcgcctggg caacttatac gctggcctca ttaatcatgg cttatcactt tcatgtgcgt
      541 aaaaacaaag gtctgagtct ttccggcatt attgccgcga ttaccggcgt tcgcccgcaa
      601 ggcccatggg gaaaactggt tgatttgatg ttcctgatcg ccactgtcgg cgcactgact
      661 atttcccttg ttgtcaccgc agcaaccttt actcgtggac tttccgcgct gaccggttta
      721 cccgataact tcaccgtgca ggcatttgtg atcctgcttt ccggcggcat tttttgccta
      781 agctcgtgga ttggtatcaa caacggtttg caacgtctga gcaaaatggt tggctggggc
      841 gcgttcctgc tgccattact ggtgctgatt gtcggcccaa ccgaatttat taccaacagc
      901 atcatcaatg ccatcggtct gaccacgcaa aacttcctgc aaatgagctt attcaccgat
      961 ccgcttggcg atggttcatt tacccgcaac tggaccgttt tctactggct gtggtggatc
     1021 tcatacaccc ctggcgtagc aatgtttgtc acccgcgttt cccgcggtcg taagattaaa
     1081 gaagttatct ggggactgat cctcggcagc accgtcggtt gctggttctt cttcggcgta
     1141 atggaaagct atgccattca tcagtttatc aatggcgtaa tcaacgtccc acaggtgctg
     1201 gaaacactgg gcggcgaaac agctgtacag caagttctga tgtcgttgcc agccggtaaa
     1261 ttgttcctcg ccgcatacct gggcgtgatg attattttcc ttgcctcgca tatggatgcg
     1321 gtggcctaca ccatggctgc gaccagtacg cgtaatctcc aggaaggtga cgatcctgac
     1381 cgtgggctgc gtcttttctg gtgcgtggtg atcactctga tcccgctttc catcttgttt
     1441 accggtgctt cgctggaaac gatgaaaacc accgtcgtgc tcacagccct tcccttcctc
     1501 gtcattttac tggtgaaagt cggcggattt attcgctggc tgaaacagga ttacgccgac
     1561 attccggctc atcaagttga acattatctc ccgcagacac cggttgaagc cctggaaaaa
     1621 acgccagtgc tccctgcggg aaccgtattc aaaggcgaca actgagcgcc atcatcctaa
     1681 cgataaaggt atccctatga gcaatctgag ccctgacttt gtactacccg aaaatttttg
     1741 cgctaacccg caagaggcgt ggaccattcc tgcccgtttt tataccgatc agaacgcgtt
     1801 tgaacacgaa aaagagaacg tcttcgccaa aagctggatt tgcgtcgctc acagcagcga
     1861 actggcgaat gccaatgatt atgtgacgcg tgagatcatt ggcgaaagca tcgtgctggt
     1921 acgcggtcgt gataaggttt tgcgcgcgtt ctataacgtg tgtccgcacc gtggtcatca
     1981 gttgttgagc ggtgaaggaa aagcaaaaaa tgtgattacc tgcccgtatc acgcatgggc
     2041 attcaaactc gatggcaatc tggcccatgc acgtaactgc gaaaacgtcg ccaatttcga
     2101 tagcgacaaa gcgcaactgg ttccggtgcg tctggaagaa tatgccggat tcgtcttcat
     2161 caacatggac cccaacgcca ccagcgtaga agatcagtta cccggtctgg gcgcgaaagt
     2221 gctggaagcc tgcccggaag tccaagatct gaaactggcg gcccgcttta ccacccgcac
     2281 gcctgccaac tggaagaaca ttgtcgataa ctatctcgag tgctatcact gtggtccggc
     2341 gcatccaggt ttctccgact ccgtacaggt tgatcgttac tggcacacca tgcacggtaa
     2401 ctggacgctg caatacggtt tcgccaaacc gtccgaacag tcgtttaaat ttgaagaggg
     2461 tacggatgcg gcattccacg gtttctggct gtggccgtgc acgatgctga acgtcacccc
     2521 gatcaaaggg atgatgacgg tcatttatga attcccggtg gattctgaaa ctaccctgca
     2581 aaactgcgat atttacttca ccaatgaaga gttaaccgac gagcaaaaat cgctgattga
     2641 gtggtatcgc gatgtgttcc gtccggaaga tttacgtctg gttgaaagcg tacagaaagg
     2701 gctgaaatcg cgtggctatc gtggtcaggg gcgcatcatg gccgacagta gcggtagtgg
     2761 catttccgaa catggtatcg cccatttcca taatctgctg gcgcaggtgt ttaaggacta
     2821 atgacatcgg cggcggtatt ttccgccgct gggctgattt ttgatggagt acagcaatgt
     2881 cagactatca aatgtttgaa gtacaggtga gtcaggtgga accgcttacc gaacaggtga
     2941 aacgcttcac gctggtggca accgatggca aaccgctgcc tgcgttcacc ggaggaagtc
     3001 acatcattgt gcagatgagc gatggtgata accattacag caatgcgtat tcactactga
     3061 gttcgccgca tgacacctct tgttatcaga ttgccgttcg gctggaggaa aactcgcgcg
     3121 gcggttcccg ctttttgcat cagcaggtaa aagtgggcga tcggttaacg atttcaacgc
     3181 ctaataacct gtttgcgcta attccctcag ccagaaagca tctgtttatc gcgggcggta
     3241 ttggtatcac ccctttcctg tcgcacatgg cagagctgca acacagcgac gtcgactggc
     3301 agctacatta ctgctcgcga aatccagaaa gttgcgcatt tcgtgatgag ctagtccagc
     3361 atccgcaggc tgagaaagtt cacttgcatc actcatcaac cggaacacga ctggaattag
     3421 cgcgattatt ggcggatatc gaacctggca cacacgttta tacctgtggt cccgaggcgc
     3481 taaatgaagc ggtaagaagt gaagctgcgc gtctggacat cgtcgccgat acgctgcact
     3541 ttgagcaatt tgctatcgaa gacaaaaccg gcgatgcctt taccctggtg cttgcccgtt
     3601 ccggaaaaga gtttgtggtg ccggaagaga tgactatttt gcaggttatt gaaaataata
     3661 aagccgcgaa agtggaatgt ttatgtcgtg aaggggtatg cggaacctgc gaaacagcaa
     3721 tactggaagg tgaagctgac catcgggatc agtattttag cgatgaagag cgtgccagcc
     3781 agcaaagtat gttgatctgt tgttcgcgtg cgaagggtaa acgcctggtg ttggatttgt
     3841 agtttgccaa agccggatgt ggcgctgagc gcgcccagtc cggcttcgga agattttact
     3901 gcggatattc ctgcaataaa ttgtgtaatg cttccgccat cagctcacca cgccagccgg
     3961 aaatcagctc cggcaaattg ttctgcggtt tcagtttcca gtgccagttc agcagttggt
     4021 tgatttgccg acgcgatgcc agcaattcgg cgctgatctt atgcgtttcg ctcacgtcag
     4081 taatcagcga cttaatcgct ttaaacactt tacgataacc aggcatgtcc atcaggttaa
     4141 gcatcggctg cggtaaggcc tcttccggca atgtctgcgc tttttccacc agcgccagca
     4201 gcgttttacc gtgaaagcgg atttcgctac cggataaacc caggctgtcc agttcgccta
     4261 aacttcccgg catataacgc gcgaccgacc acaaatgctc ttcacgcacg acaaagttca
     4321 ccgccagatc acgctctcgc gccttgcgca gtcgccagtc ggctaacagt tgcagacagg
     4381 ccagttggcg tgtgcgtaac tgccaggcat tggtgatatc acgccaggca tcttccggcg
     4441 caacgacttc ctgacgacgc atttgcatca ggcggcattc atccagcgcc gcaggtagcc
     4501 agccggaggc ctccgtttct accataagct tggcggtgat cggtaacaga taccagacat
     4561 ccgccgctgc gtattcacac tgacgttcgg tcagcggtct ggccagccag tcggtgcgcg
     4621 attcactctt gtccagcgta acgccggaat actcttccac catggaagcg aaaccccatg
     4681 acatcgggcg tccgcagaag gcagcaagga tttgcgtgtc aatcaagggt tgtggtaatt
     4741 cgccaaagac attgaggaac acttccagat cttcactgcc tgcatggaga aattttgtga
     4801 tggacggatc gcgcaggatc gctttcagcg gtgaccagtc ggtgatcccg agaggatcaa
     4861 ttagcgccag atgctcgcca tcgaaaagtt gaatcaaccc cagctgcggg taataagtgc
     4921 gcgtacgaac aaattcagta tccagggcta tcgccggaaa ggcacggacg gcttcacaca
     4981 aagaagccag cgcatcgtcc gtggtaatca tttggtaatt caaattgttt tctctttagt
     5041 gggcgtcaaa aaaaacgccg gattaaccgg cgtctgacga ctgacttaac actcaggctt
     5101 tattgtccac tttgccgcgc gcttcgtcac gtaattctcg tcgcaaaatt tttccgacgt
     5161 tagatttcgg taactcatca cgaaactcca ccagcttcgg tactttgtag cccgtgagct
     5221 gacggcggca aaaggtcacc agtgactctt cggtaagcga tggatctttt ttcactacga
     5281 agattttcac cgcttcacca ctggagccgg aaggtacgcc aacagccgcg acttcctgta
     5341 cgccaggatg ctgcatgacg acatcttcaa tctcgttggg atagacgtta aaaccggaaa
     5401 ccagaatcat gtctttttta cgatcgacaa tgcgcaggaa cccttcttca tccatcaccg
     5461 cgatgtcgcc ggtgtgtaac cagccatttt tgatgatctc atctgtagca tccggacgct
     5521 gccagtaacc cagcatcacc tgcggtcctt tgacacaaag ctcacccggt tgacccggtg
     5581 gtacttcatt atcatcatca tccaccagtt tggcttccgt cgacggcacc ggcaaaccga
     5641 tgctaccact atgataatca atatcatatg ggttaacgct gaccagcggc gcacactcgg
     5701 taaggccata gccttccagc agatactgtc cggtcagttt cacccaacgc tctgccacca
     5761 cttgctgcac tggcatccct ccgcctgcgg aaagatgcag actggagaaa tccagctgct
     5821 ggaactcttt attgttcagc aacgcattga acaaggtgtt aacgcccgtg atagcggtaa
     5881 acggatattt cgctaactct tttaccaacc ctggaatatc gcgcgggtta gtgataagca
     5941 ggttctgccc acccagttcg ataaacagca ggcagttaat ggtcagggca aaaatgtgat
     6001 acagcggcag cgccgtcacc accagctctt tgcccggatg caacagcgga ccataggtcg
     6061 cgttaacctg ttccaggttc gccagcatat tgcggtgagt cagcatcgcg cctttcgcca
     6121 caccagtggt gccgccggtg tattgcagaa aagctaaatc ttccggcacc agttcgggtt
     6181 tgacgtactg catccggtag ccgttacgca gtgcgctacg aaatgaaatg gcatctggca
     6241 gatggtattt cggcaccaaa cgcttgatgt atttaacaac gaaattgact accgtgcctt
     6301 ttgccgtaga tagctgatcg cccatacggg tcagaattac gtgctgaacg gcggttttat
     6361 caaccacttt ttccagtgtg tgagcaaagt tagacacgat aacaatcgcc gatgcgccgc
     6421 tatcgttaag ctgatgctca agctcacgcg gggtatacaa cgggttaacg tttacgacga
     6481 tcatcccggc acgcaaaata ccaaacagcg ccaccggata ttgcaataaa ttaggcatca
     6541 tcaacgcaac gcgatcgcct ttcttcagcc ccaacccttg ttgcaaataa gcggcaaacg
     6601 cgcgactgcg ttcttccagc ttgcggaagg tcattacctc ccccatattc acaaacgcag
     6661 gttgatcggc gtagcgcgcg accgactgct caaacatatc taccagagat tgataacggt
     6721 cagggttgat ctccgtcgga acgtccgcgg gataacggtt aagccaaacc ttcttcaatt
     6781 cttcacctct aaaatgcgtg ttcgtcgtca tcgcaacccc aaatgatata catgccgtta
     6841 acataatatt aactcatcat accagcttga taattaccca acgaaaaggt tgcgaagcgc
     6901 gtcactattt atttttatct ttaccgtaag aatgcagaaa cagcggacca gccgctgttt
     6961 ctttttcttt aaaaacaagc aattattcag ttacaactgt ttgtactctc gcggggccgg
     7021 gattatacca gccccatccg ccgtagccat agggccagcc acgaccgcca taaaaccatg
     7081 gatcaatcgg ctgaggcggc ataatcacct gctgggttaa atgccaacgt ttgtaacccg
     7141 ttacttgcat caccataaat ttatagggcg tattgccgat tttgccgtca accgcaccag
     7201 tgattggccc gactaccgta acaagttgtc cacggaaatc caccgggtcc agaaaaccgt
     7261 tcacatcggc ataaatgcga ccgcgagaag gttctcccag cgtcggtctg gctccgctgt
     7321 ccagcggtac ggtagcaatt tccaggcggg ttttcccttg ctggttttgt accgcaacca
     7381 ctttgccacc aaagcgtgcc tcctgaccaa cgtacagctg cggcgcactc atcacccgaa
     7441 ctaaatcttg ttgcggcgtg ggactgctgc ctttaatggc gtccggcaca gtgacacaac
     7501 cgctcagcat tagcgcaaac gtacctgcca gtatgccttt gataacattc ttttgaaccg
     7561 ccatgatgcg actccttttt ttctcagggc atactcttaa gattcattct ttgcccggaa
     7621 gtttcttcca tgcgacgttg ttacgtaaat aaaccggttc ggcatgttcc accgccaccg
     7681 ttttaccctc ggcaaacatc tgacacgcaa tcggcagcat atcttccgcc gctggcagta
     7741 aaacttctcc gtcgcgcaaa accagcccgc tctctttacc gagatccggc caggcttgcc
     7801 agcccgtccc taccgttacc cattcgccgg aaagctgttg cattcgttca tgaacgagtt
     7861 cgggtttgag tacggcttcg gtttcttcac cgcgccagat accgttttca tcacgctgat
     7921 attcggccca gtaaacttcg cccattcgcg cgtcaatggc tgccagcacg cgggttgcgc
     7981 cgtttttgcg ccacgcccct tgcgccatcg tcattagcgt ggagacgcca atcatcggta
     8041 gctctgcgcc aagtgccagc ccttgcgcaa tgccaatacc aatgcgcacg ccggtaaagc
     8101 taccggggcc gcgcccgtaa gccagagcgt taatatcagt cagggaagtt ccgctggtgg
     8161 tcaggatatc ctgcaccatc ggtaagattc gttgagtatg ttcacgaggg caaagctcaa
     8221 aatgagcgtt gacagtaccg tcgttccaca gggcgacaga gcaggcctct gtcgcggtat
     8281 cgatagccag aattcgcatg ggtcttcgtg cttagatcaa taaaaaggcg cgcatcatac
     8341 catactccgt aacaaattac ctggaggatg gtatcgcaag gaaacgaacc gcacgggcaa
     8401 tgtcacgagt gcgtggcgcg ggcggcagac tggcgagaaa cgtcgcgccg taaggacgca
     8461 tcaccagccg attgtcacaa atcaccagca cgccacgatc gtcggcgtcg cgaatcagtc
     8521 gccctacccc ctgtttgaga gtaatgacgg catctggtag ttgcacttca tcgaacgggt
     8581 cgccaccgcg caaacgacaa tcttccatgc gcgcttttaa cagtggatca tccggcgagg
     8641 taaacggcaa cttgtcgata atcaccaatg acaatgtatc gccacgcacg tccacccctt
     8701 cccagaaact actggttgcc acaagaagcg cgttaccggc gctgacaaat tgctgcaaca
     8761 gttgcccttt gctggtttcc ccctgcaaca aaacgggaag cgtcatggta gcgcggaact
     8821 gctcggccag atcacgcatc atggcgtgcg aggtacaaag cataaaacaa cgaccgttgt
     8881 tagcttcgat gatcggtcgc agcattgccg ctaactggcg agcagaacct ggctggttgg
     8941 tttgcggcag attgcgcggc acacagagta acgcctggcg gctgtaatca aacgggcttg
     9001 gcaatagcaa cgactccgcc tgttctatcc caagccgcga ggtgaaatga tgcagatcgt
     9061 cgttcaccga cagcgttgct gaggtgaaga tccagctacc aggcttttgc gccattaact
     9121 ctttgaattt atccgccacg ctgagcggcg tgagagccag agtaaaatgg cgcgaagtgc
     9181 attcgtacca gtagctgtag cccggctgat tgatctcttt tagccgcttc agccgtgtgc
     9241 gatacaacgt agcgcgctca aatgccgcat ccagcaaggc ggaacgcccc agtgacagtt
     9301 tcgccacgtc ataacaaagt tccagggtgt catcgagcag taaaaatgcc cgctgaattt
     9361 gcggattagc taacagctca cgcaggttac cgcgataacc tggctcaccg agttgcagac
     9421 gaaaatcctg cgcactctgg gcaagacgat cagcgcactt ttgtaactgc tgggtgtctt
     9481 ttaattcggt gcggtaagcg atggtgatgt cttttgccag gtcgagcagt tgtcgactgg
     9541 agagtgactg accaaaatac tggctggcaa tgtccggtag ctggtgggct tcgtcgaaga
     9601 tcatgacgtc cgcttccggg atcagttcgc caaatccact ctctttaacc accatatccg
     9661 ccagaaagag atgatggttt accaccacca catcggcgtc catcgctttt ttacgtgctt
     9721 tgaccacaaa gcaatcttta tacatcgggc agtcgctgcc aagacagttg tcgttggtgc
     9781 tggtgaccag cggccacgcc tgtgaatctt ccgccacgct gacgcaggtg ctgatatcac
     9841 catcgactgt ttgattagac caggagcgca gcaggatcac atcgcttaag atttgtaccg
     9901 gcagatcgcc ccccgccagc gcctgctgtt cgagacgctc gaggcagagg tagtttgagc
     9961 gccctttcag cagcgccacg ttgcccgtat atttcaatgc ctttgagact gttggcaaat
    10021 cgcggctgta gagctgatcc tgcaacgctt ttgagccggt cgagataatg actttctttt
    10081 tcgcccgcag cgcaggagcc aggtaagcgt aggttttgcc cgtaccggtt cctgcttcca
    10141 ccaccagcgg ctggcctttt tctatcgcct gggtgacggc taccgccatc tgtcgctgtg
    10201 gttctcgcgg cttaaagcct ggtatcgctt tcgccagctg accgtctggt gcaaaatcgt
    10261 ccgtcacact accccctgtt gatttgaaca gggattatgt caggatgagg gcgctttcgc
    10321 cagttgaagt ggtgacggcg acctcacatt gtggcagtct ttgcagcacg aaaatggaag
    10381 gataatgagg acaaaatgat gactatcgtt cgtatcgatg ctgaagcccg ctggtctgat
    10441 gtggtaatcc acaacaacac gctctactac actggtgtac cggaaaacct cgacgccgat
    10501 gcctttgagc aaaccgccaa cacgctggcg cagattgatg ccgtgctgga aaaacagggc
    10561 agcaataaat cgagcattct ggatgccacc attttcctgg ccgataaaaa cgacttcgcg
    10621 gcgatgaata aagcgtggga tgcctgggtt gtcgcgggtc atgcgccggt gcgctgcacg
    10681 gtacaagcgg gtttgatgaa cccgaagtat aaagttgaaa ttaagattgt ggctgcggtg
    10741 taagctttat cgaagcaaaa taagtcagac gataatttat cgataatact ggtcggtttt
    10801 acataaatcg ac
//
LOCUS       DAFNBJ010000162        10804 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000162 DAFNBJ010000000
VERSION     DAFNBJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.162"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(176..1135)
                     /locus_tag="L2X49_004008"
     CDS             complement(176..1135)
                     /locus_tag="L2X49_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HBN4799014.1"
                     /translation="MIKKPVIGISGCLAGSAVRFDGGHKRADFLMDKLVEWVTFRPVC
                     PEMAIGLPVPRPALRLVRSMQGNIRMCFSHDQNEDVTERMTEFSRSYMDKLKDVSGFV
                     VCAKSPSCGMERVRVYDENGNRGRKDGVGLFTSTLMEKFSWLPVEEDGRLHDPVLREN
                     FVERVFALHELNHLYKEKLSRRELLAFHSRYKLQLLAHSQAGYKDMGPFVAAIHEWAD
                     LESYFEVYRDKLMAILRKPASRKNHTNVLMHIQGYFSNYLSTRQRKELSEVILNYRSG
                     TLPLLAPLTLLKHYLGEYPNDYLLTQNYFDPYPDELALRLMVN"
     gene            1328..1852
                     /locus_tag="L2X49_004009"
     CDS             1328..1852
                     /locus_tag="L2X49_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HBN4799015.1"
                     /translation="MKLWPVLTGIALSFTLIACKAPTPPKGVQPITNFDANRYLGKWY
                     EIARLENRFERGLEQVSATYGKRNDGGIRVLNRGYDPTKNKWSESEGKAYFTGDTKTA
                     ALKVSFFGPFYGGYNVIKLDDEYKYALVSGPNREYLWILARTPTIPDKVKADYVRTAQ
                     KLGFNVNELLWVKQ"
     gene            complement(2008..2385)
                     /locus_tag="L2X49_004010"
     CDS             complement(2008..2385)
                     /locus_tag="L2X49_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBN4799016.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVGGMTFMALARKHGRSDCWVGRMLQKA
                     EGVVEGMLMVLDLRLEMDADCSK"
     gene            complement(2471..2611)
                     /locus_tag="L2X49_004011"
     CDS             complement(2471..2611)
                     /locus_tag="L2X49_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HBN4799017.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2608..2970)
                     /gene="rusA"
                     /locus_tag="L2X49_004012"
     CDS             complement(2608..2970)
                     /gene="rusA"
                     /locus_tag="L2X49_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HBN4799018.1"
                     /translation="MNTYNITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2967..3257)
                     /locus_tag="L2X49_004013"
     CDS             complement(2967..3257)
                     /locus_tag="L2X49_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBN4799019.1"
                     /translation="MADLRKAARGRECQVRTPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWMKEGVIKA"
     gene            complement(3250..3420)
                     /locus_tag="L2X49_004014"
     CDS             complement(3250..3420)
                     /locus_tag="L2X49_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799020.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3420..3875)
                     /gene="ybcN"
                     /locus_tag="L2X49_004015"
     CDS             complement(3420..3875)
                     /gene="ybcN"
                     /locus_tag="L2X49_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HBN4799021.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLEDGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKEALKHTYLGYETKELVDVVTGEITTI
                     QSLRHTSDLDAGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3872..3973)
                     /locus_tag="L2X49_004016"
     CDS             complement(3872..3973)
                     /locus_tag="L2X49_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799022.1"
                     /translation="MRTYNPNSLLPSQMQKCTCDFLHPAFDLCGGEA"
     gene            complement(4066..4518)
                     /locus_tag="L2X49_004017"
     CDS             complement(4066..4518)
                     /locus_tag="L2X49_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799023.1"
                     /translation="MKRLFVIAPLLVLVGCAQNISPNSYSVGSVGMVNRTIAGTVISA
                     RGVDISGTSALGGTAGAAVGATAGSALGGGVRSNIVGAVGGAVIGGIAGAAIESSATK
                     QTGMEYVVETENGNLMTIVQGKDPLFTQGSKVLVLYGNPSRIITDPRH"
     gene            complement(4515..5075)
                     /locus_tag="L2X49_004018"
     CDS             complement(4515..5075)
                     /locus_tag="L2X49_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine acyltransferase"
                     /protein_id="HBN4799024.1"
                     /translation="MKKIILLAMIIGSLTGCASVPPLNFSTPNVGVSQKKIDAEIKSL
                     TVSLARPDEQKGDITAGMEAITPIWRESLQEALDRMTIFRDSSPNTVSLNVKVLALDV
                     PAFGVSMTTKAIARYEIINRANGDIIYTQDIESTGTVPASYAFYGIVRARESVNRAVQ
                     NNITQFLQALESVDLSRPMFPVRVAK"
     gene            complement(5560..5853)
                     /locus_tag="L2X49_004019"
     CDS             complement(5560..5853)
                     /locus_tag="L2X49_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HBN4799025.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARTG
                     ILVIDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLAFYRGNFQ"
     gene            complement(5850..6551)
                     /locus_tag="L2X49_004020"
     CDS             complement(5850..6551)
                     /locus_tag="L2X49_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HBN4799026.1"
                     /translation="MKNIAAQMVNFDREQMCRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(6548..7567)
                     /locus_tag="L2X49_004021"
     CDS             complement(6548..7567)
                     /locus_tag="L2X49_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBN4799027.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQNGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKPKLDLTNTDWI
                     YGVDL"
     gene            complement(7564..8103)
                     /locus_tag="L2X49_004022"
     CDS             complement(7564..8103)
                     /locus_tag="L2X49_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HBN4799028.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAEDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(8173..8403)
                     /locus_tag="L2X49_004023"
     CDS             complement(8173..8403)
                     /locus_tag="L2X49_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN4799029.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            8508..9197
                     /locus_tag="L2X49_004024"
     CDS             8508..9197
                     /locus_tag="L2X49_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4799030.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            9320..10069
                     /locus_tag="L2X49_004025"
     CDS             9320..10069
                     /locus_tag="L2X49_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799031.1"
                     /translation="MECVASSNSAIPNVVEVIRRINEGSTQPFLCKCDDGQLYVLKSK
                     PSMPPKNLLAEFISACLANDIGLPLPDFKIVFVPEELIEYSPDLQQQICTGYAFASLF
                     IDGAIALTFTQSRNETIIPVEQQKLIYVFDKWILNADRTLTDKGGNVNILYDISNDKY
                     YLIDHNLSFDQNAGPEDFSVHVYGPGNRKWQYDLVDRVEYRQRVVNSLHKLPAILDEI
                     PEEWIVDEEFLPFVCTTLDKGDCDEFWSAIE"
     gene            10066..>10804
                     /locus_tag="L2X49_004026"
     CDS             10066..>10804
                     /locus_tag="L2X49_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HBN4799032.1"
                     /translation="MTTPCLYSIVRYAPYAETEEFANIGVLLCAPKENYFDFQLTKRN
                     DSRVKNFFHDDCIFPVAKDSIQRELQFAKMHATQIVGHQQLAQFFRYFTNKKESIFQF
                     SSTRVILSENPKEELARIYNKYVNHSDYTKERREDVLARELKRSIDRIDGLKNVFKQA
                     TIDGYFAKFSMPLVAKKHDRIQCAIKPLAFTQAEPGKMMEHSDTWVMRITRAAEENLL
                     SLDDILFTIETPESPNSGQSKVIDIIKR"
BASE COUNT         2943 a         2566 c         2291 g         3004 t
ORIGIN      
        1 taatatcata tattttgact ggcatgttta ccaactttaa gttgcatctc aattgtttct
       61 tcagcgtaaa cagagttttt atacaaactg acactctggg tatcatagtg tagtttttac
      121 gattgtaaat atcctgcatg caggaactca tccttttgga tgatatcgca tacaattaat
      181 ttaccatcag tcttagagcc agttcgtccg gatagggatc gaagtaattt tgtgtaagca
      241 agtaatcatt aggatactca cccagataat gcttcagcag agtcaacggc gcaagaagag
      301 gtaatgtgcc agaacgatag ttaagtataa cctcgctcaa ctctttacgc tggcgtgtac
      361 ttaagtagtt actaaaatac ccctgtatat gcatcagcac attcgtgtga tttttacgtg
      421 atgcaggttt tctgagaatc gccatcagct tatcacgata cacctcaaag tatgattcaa
      481 ggtccgccca ctcgtgtatt gcagccacaa atggtcccat atctttatag cctgcctgac
      541 tatgcgccaa caactgaagc ttataacgac tatgaaaagc taataactct cttcttgata
      601 atttctcctt gtaaaggtga ttgagctcat gcaaagcaaa aactctttca acaaaattct
      661 cacgaagcac tggatcatgt aatcgcccat cctcttcaac cggtagccag gaaaactttt
      721 ccatcaaagt gctcgtaaat agtcccactc catctttacg acctcgatta ccattttcat
      781 catagacacg cacgcgctcc atgccacagc tgggagattt agcacaaacc acaaaccccg
      841 atacatcctt taatttgtcc atataagaac gactaaactc tgtcattctc tctgtcacat
      901 cctcattctg gtcgtggctg aaacacatcc gtatatttcc ttgcatcgag cgcacaagac
      961 gtagagcagg acgcggaact ggcagcccta tagccatttc cggacatact ggtctgaatg
     1021 ttacccattc cactaatttg tccattaaaa agtcagctct tttgtgacca ccatcaaaac
     1081 gaacagcaga accggccaaa caaccgctga ttccaatcac aggttttttt atcatattct
     1141 cccccttgac taattcatta acacataaac tgtgtagtgc acggaataaa ttgcctttct
     1201 ggcgtcatca ctgacaattt ttctgttatg gactattcct aatatagtat gaaagttctt
     1261 taagtgatcg gtcgtaatca tctatctttc atacttactc tcaactatca aaagtacagg
     1321 atttattatg aagttatggc ctgtgttgac tggcattgca ctctctttca ctcttatagc
     1381 atgtaaggcc ccgacaccac ctaaaggtgt gcagccgatt acaaattttg acgccaaccg
     1441 ctacctcgga aaatggtatg aaatagctcg cctcgagaac cggttcgaac gtggtctgga
     1501 acaggtcagc gctacttatg gaaaacggaa cgacggaggg attcgtgtac ttaaccgtgg
     1561 atacgatcca acgaaaaata aatggagcga gagcgaaggt aaagcatact ttactggaga
     1621 tactaaaact gcagcattga aggtttcgtt ttttggcccc ttctatggtg gctataatgt
     1681 aatcaaactg gatgatgagt ataagtatgc tcttgtcagt ggtccgaaca gagaatacct
     1741 atggattctg gcaaggaccc caactattcc agataaagta aaagcagact atgtgcgaac
     1801 cgctcaaaag ttgggattca atgtcaatga attattatgg gttaaacaat aaaatcccta
     1861 cccgaaataa tacttattag aaaaaaacca gcctttgggg aggctggcta aatcaggaaa
     1921 caagctgtta tatgataata actacgttgt gattccaaca tttaaaatgt tagactaatg
     1981 acaatcagac agcaactttt cctttaatta tttcgaacaa tcagcatcca tctccaatcg
     2041 gagatccaac accatcagca taccctccac tacgccctca gctttctgaa gcatcctgcc
     2101 aacccaacaa tcagatcgcc catgcttacg tgcaagcgcc ataaaagtca tgccgccgac
     2161 ataatagtcc accaataaat catgcaaatc gctgttgttc tttttcagac gggccatgca
     2221 cccgcaaatg atcatcgcat catcgtcaca acattgcggg cgagatttta cttttgaagg
     2281 aattaatccc ttaaaaccgg cggcaatgga cgaccaagtc acatcttcat gattattagc
     2341 cgcccacgct ccccaacgct caagaaccat ctgaatatca cgcatcaact tactccacaa
     2401 aaatcagacc agaacgccaa tcacaagcaa aaatcaacaa aacagtatta gttgattgtt
     2461 atctctgact tcatactcct gctcctgtca gggttttggc gtaattcttc agtattcggt
     2521 aatcggtcaa aacagaaccg gggaaacgat ataagcgcag atgcccccag cggtggcgaa
     2581 gaagttctgc catataaaac tcaaacatca ttcattcccc atttcggtga tggtcagttc
     2641 cagcctccca cctttggtaa caggcatctt cacaacgcgg taatcaacga cctgagcatc
     2701 atccagccag aaacctgctt tagtgagtgc gtcaaaagcg gctttttgca gattatccag
     2761 gtcacggcga cggcgatccg gcatgtggca ctcaatgcgg attttcacag gcatagccag
     2821 gccgatatcc agcattgcgt ttttaatgat tcgggcgacg ttatcgcggt atgcctgccc
     2881 ctctgcgctg acgtgcgtgc gcccgcgatt atggcggtaa tagcgattat tgctcggagg
     2941 ccagggtaat gtgatgttgt aggtattcac gccttgatta ccccctcttt catccagata
     3001 acctgcgttc tcgccatacc ttccagcgcg cattcttttg catatgcggc atcgacaaaa
     3061 tgtgtgcggc ggtcgatttc gtcgtggcag gcagaacatg caatggtggc aatcaggtct
     3121 ggcggtttgg taccggtgcc gcacaatcca gtcagccgga tatgtgccag tacagacgtt
     3181 tcagggttgc cattacatac gccaggggtt cttacctggc attcccgacc acgcgctgct
     3241 tttctcaaat cagccatgat tcctccttgc tgccagtcgc aaccattttt tatcaaccag
     3301 gctggcggta tatccgagca gtgttggtat ttcggatggc ttcagctcag gtttacgctt
     3361 acgacgattt ggtactctgt agatgtgtcc gttcatgaca cgaataagcg gtgtagccat
     3421 tacgcctcct gcttgtcgcg cagcagctgg aactcgcagc tctgcggaat agtcaggtgg
     3481 cagccaatat tcatcgccca ggcttcaacc ttacacagga agacatacat ctctccggca
     3541 tcaagatcgg aggtatggcg taacgactgg atagtggtga tttcaccggt tacgacatca
     3601 accagttctt tggtttcata accgaggtat gtgtgtttga gagcttcttt tacccatgct
     3661 gcggtagcga acgatttccc cctgctgata aggtattcac tgatttcgct gtaccacatg
     3721 tggctgagtg cattctggga aagactgcgt ttctcacgcc acggtttaag caccatgcga
     3781 aagcattttc cgtcctccag ataaggctgg atctgctggc cgatagcggt gaagttgccg
     3841 cgatgtaatt tgatgccatc ttgtggtagg ttcacgcttc acctccgcag aggtcaaacg
     3901 caggatgcaa aaaatcgcag gtgcatttct gcatctgtga agggagaaga gagtttggat
     3961 tgtatgtgcg cataaacgtc cccgtttagc gcagaagtca ccggagttgt tcaagctccg
     4021 atgactttat tattacgaat tgattttaca aaatcaaaag gtatgttagt gacgcgggtc
     4081 tgttattatg cgagaagggt ttccgtataa aacaaggacc ttacttcctt gagtaaataa
     4141 cggatctttg ccttgaacaa tggtcattaa attcccattc tcagtttcga caacatattc
     4201 catgcctgtt tgttttgttg ctgaagattc gattgctgcc ccggcaatac caccaatgac
     4261 tgcaccacca acggcaccaa cgatattaga acgaactccc ccaccaagcg cagaaccagc
     4321 ggttgccccc acggcagccc cagcagtccc gcctaacgcg gaagtcccac tgatatcaac
     4381 ccccctggca ctaataactg taccagcgat agttcgatta accatgccca cagagccaac
     4441 agaataacta tttggcgata tattttgtgc gcatccaacc aacactaaga gtggagcaat
     4501 tacgaataat cgcttcattt agctacccta acaggaaaca ttggacgaga aagatcaaca
     4561 ctttctaatg cttgcaagaa ctgcgttatg ttgttttgca ccgcgcgatt aacagattcg
     4621 cgtgctcgaa caataccgta gaatgcgtaa ctggctggaa cagtaccggt agactcaata
     4681 tcctgcgtat atataatatc accattcgca cggttgatta tttcatacct tgcaattgct
     4741 ttagttgtca ttgaaacacc aaaagcagga acgtcaagag ccaacacttt aacatttaag
     4801 ctaaccgtat ttggtgaact atcacgaaaa atagtcattc ggtcgagtgc ttcctgcaaa
     4861 gattcacgcc aaattggagt tatagcctcc ataccagcag tgatatcccc tttctgctca
     4921 tctggacgag caagtgatac cgttaatgac ttaatttcag catctatttt tttctggcta
     4981 actcccacgt taggtgttga aaaattcaat ggtggcacac tagcgcaacc tgttaaagaa
     5041 ccaataatca tggctaataa tattatcttc ttcataaatt taccttattg ttataaccaa
     5101 aggaattata aagtaaaaaa gttcactatc actagccatt aacgacatca atttcagaga
     5161 aacatggtac tcatttccac aaatttgaca caagtcattt tcatctacat attccatcat
     5221 acttgatgca tatgttattg aagcctctat cctatccgtt cataatagca atagttaccc
     5281 gggtgatagt acctctatga ttactcgtct ttctgattga ttggattaaa tatgcgcgcc
     5341 aaaatttatc aactttcgtt atggatattt atttcgtttc tagcgatcta tgcctttatt
     5401 atctataaag gttcttatat tggagtagca ttgcatcaaa ttgcttggat catcattatt
     5461 gcctctggct tgattgctag gctaactaaa ccaaagcaaa aaccaatttc gtccaataat
     5521 tagacatgta ttaaaaaatg atatttttat gtacatagtc tattgaaaat tgccgcgata
     5581 aaatgccaac acccgcttca tcgcggcact ctggcgacac tccttgaaaa tcagattcgt
     5641 gctcaccttt ccttcccgtt cttccctggt agcgaaccgg taatacaccg ttcgccagac
     5701 cttaccatca ataactaaga ttcctgtccg cgccatttta gccgcagcct ggtttatgct
     5761 ggttactgtt gcgcctgtta ccgcagcaac gtcctgcgca cagaagctct tatgcgtccc
     5821 caggtaatga ataattgctt cttttcccgt catacactgg ctcctttcag tccgaactta
     5881 gctttgattt ctgcaatctt cgccagagcc tgtgcacgat ttagaggtct accgcccatg
     5941 acaggaagtt gttttactgg ttcagggatc gcctcaccac ggttaattct cgcagtcata
     6001 tggacaagct catctgcggc cttacggcgt aattccgcat cagtaagcgc attggcccgc
     6061 atgttctgat acaggttggt aaccagccag tagtgcgcgt ttgatttcca cggataagac
     6121 tccgcatccg gatacaggcc tcgcttccgg caatactcgt aaaccatatc aaccagctcg
     6181 ctgacgtttg gcagtccggc gataacggat gcttcttccc ggcaccatgc aacaaactgc
     6241 ccgggtgatg gcaggaatgg tcgattctgc cgacgggcta cgcgcattcc agcgttaacc
     6301 tgttccattg tggtgatccc gttttcccga aaagccagca cccactggcg gcggatttcg
     6361 ttcagttcgt tctggtcccg gttagccagg ctcgctggga aagttgccag taactggctg
     6421 aatacaccgt tgattatctg cgctacctgc tgtacctgcg gcttttcgtc gtactgttcc
     6481 ggcatgttat tggcgatccg gcacatctgc tcacggtcaa agttaaccat ctgtgcggcg
     6541 atgtttttca taaatccacc ccgtaaatcc agtcagtgtt cgtcaggtcg agttttggtt
     6601 tgccggctgt cacgccagcc tgttgcttgt ttcggttgat ttcgagctgg gtccacttgt
     6661 cgcggagttt ggccggactc agcacgttac cggaccagaa gttgtcctgg caggcccagc
     6721 ggaacagtac acacatgtcg cggtggttac gtccatcacg ttcacgcatc agacggatat
     6781 cgttagccca ccctgcaaaa ttcggttttc tggctgatgg cgcgatggtc ttcaccatgt
     6841 caaacatcca ctctgcggcg gtcaggtctt ctgctgtccc ccacttgctg ccgttctgaa
     6901 tcgcagcatc cgctttcacc acaggaaggt cgttttctgg caggtcagag gattcgccag
     6961 aattctcgga cgaataaggt tttatattgt cttttgttag tttgtctttt gtgtttacct
     7021 gattcgggta agtgccttta cctgatttgg gtaaactttt cttacctgat tcaggtaaat
     7081 ttacctcttt caggtaaact ttatttttct tacctgattc gggtaatgtt gaccattcac
     7141 tgaccacatt attaatgcct atattccgcc cgctctgaat aaaaatccca cgctttacca
     7201 gaacactttt tgcagcagaa cacttgtgcg gcaatatccc ggtcaactcg gaaagttgct
     7261 cgttgctcac ccaatccagt tttttattaa agccatatgt tttgcgcatg acagccagga
     7321 agaccagaag ctggtgctgt gttaatccgg ccagcattac agcttccagc aactcatttg
     7381 caatgcgcgt ataaccatca tcgagatctg ccacgcgcgg ctccttttgt gccgcatccg
     7441 gcactggaaa attgaatatc tcagcagtgt ttgccataat tcctcccgca atgagtgtgt
     7501 tacgatttgc acctgaaagt cggttctgtt ccagcagacc ggctttcgcc atttctgaac
     7561 ctgtcatatc gcccccagca tggtagtaac catcgccatc aatggaccag ccagatctgg
     7621 gtccacacga aacatcgaca caataccttc actaatttcc ttcagtttct ggtggcgtgg
     7681 tgcgttgaga atgacagcct gttttgcctc actgagttcc ttttccattt cagccaacct
     7741 agccatgaag ctatcctgct caaccaggta accgcgatat tccagcggta gtaccgccag
     7801 aattgccggg gtcagttcac gcacgttatt tcggtatttt tcagaatcga atttgttatc
     7861 gaggaagcgg aacagcttct ggcgtgcacg gctgacatca tcagggaaat cgatggtgcc
     7921 gccgccctgc tcccgatact cattcacaat gagtgtggca acgacatcct gattatcttc
     7981 agccgaccaa gcgcggacgg catcacggat tttttcgtgg cctggcacct gttttgtttg
     8041 agaacgattt atcaccgcag tcgggctaaa tccgctagtc tgttggtatg taagtggttg
     8101 cataattgac tcctttagtt tgaattgact gttaagttga ttgcttattg ttaaagagcg
     8161 tgaaatggaa atttaagctg cgttcttttc ggtgtgtgga aacaacttcg gaagatccgg
     8221 gcgaatctgg tatgccttca ctactccacc agtagccgta acaatgctgc cgacatgttc
     8281 aggggatacc tttgctttgt tgtgaagcca cttatagacg gcctgctgtg aaacttcgca
     8341 agcagcgccc agtttctttt gtgaaccaac gatattgatc gctgttttga tagctgggtt
     8401 cataacaacc tccgtggtta atttgaatca agattaaaac tatggttgtt tttagtcaac
     8461 aaccattttc gtttgatgga ataaaacctt ggttgtacat ttggactatg aaaacaacac
     8521 tctcagaaag acttaaagaa gccagattag cgcgaggcct tacacaaaag gcgcttgggg
     8581 atttggtcgg ggttagccaa gctgctattc agaaaatcga aacagggaaa gctaatcaaa
     8641 caactaaaat cgtggagatc gcgaacgctt tgggtgtgcg cgcagaatgg ttatcttctg
     8701 gcgttggaaa tatgtcagac agtacagtgc aaccaataca atcaactgtc agccattcca
     8761 aatacttcaa gattgacgtt cttgatatag aagtcagtgc tgggccggga gtcatcaacc
     8821 gtgagtttgt agaagttcta cgctcggttg agtactcgtt tgacgatgct cgtcacatgt
     8881 tcgatggtag gaaggcggaa aatatccgca tcattaacgt gcgtggtgac agcatgtcag
     8941 gaacgatcga accaggtgat ctgctgttcg ttgatatcac agttaaatct ttcgacggtg
     9001 atggtatcta tgcgtttctg tacgacgaca cagcccatgt aaagcgcctg caaatgatga
     9061 aggataagct gctggtcatc tctgataaca aaagctactc accgtgggac ccgatcgaga
     9121 aagacgagat gaaccgggtg ttcatcttcg gtaaggttat tgggagcatg ccgcagacat
     9181 ataggaagca tggttaaagt gaggctaaaa aacagttaca gcaataggcc tgttgttttt
     9241 ctttaaacac gcagtgttaa accgctcttt gagatgcgga gtaatgagat ggaagacttg
     9301 aatcacataa gggttagtga tggagtgcgt agcgagcagc aatagtgcaa tacctaatgt
     9361 cgttgaagta atacgtcgca tcaatgaagg ttccactcag ccatttcttt gcaaatgtga
     9421 tgatgggcag ttgtatgttt tgaagtcaaa accatcaatg cccccgaaaa atctcttagc
     9481 tgagttcatt tcggcgtgtt tggctaatga tatcggcctt cctttacctg actttaaaat
     9541 cgtatttgtg ccagaggaac ttatagagta ctcacctgat ctgcagcaac aaatttgtac
     9601 aggatatgcc tttgcttcat tgttcattga cggtgcaata gcgttaacgt ttacgcagtc
     9661 aagaaacgaa acgatcatcc cagtcgaaca gcaaaaatta atctatgttt ttgataaatg
     9721 gatattaaat gcagacagaa cgcttactga caaaggtgga aacgttaaca tcctttatga
     9781 catcagtaac gataagtatt atctgattga ccataatctc tcatttgatc agaatgctgg
     9841 acctgaagat ttttctgtgc acgtgtacgg ccctggtaac cgcaaatggc aatatgattt
     9901 agtggatcgc gtagagtacc gccagagggt cgttaacagt ttacacaagc ttcctgctat
     9961 ccttgacgaa attccagaag agtggatagt agatgaggag tttttacctt ttgtctgcac
    10021 tacgctagac aaaggtgatt gtgatgaatt ttggagcgca atagaatgac aactccatgc
    10081 ctatatagca tcgttcgcta tgcgccttat gcggagactg aagaattcgc aaacataggc
    10141 gtacttctgt gcgcgccaaa agaaaattac tttgatttcc agctcacaaa gcgaaatgac
    10201 tctcgtgtaa agaatttttt ccatgatgat tgtattttcc ctgtagcaaa agactcaata
    10261 caaagagaac tacagttcgc aaaaatgcat gcgacccaga ttgttggaca tcaacaactt
    10321 gcacaattct tcagatattt tacaaacaaa aaagaatcaa tttttcagtt cagttctacg
    10381 agagtgattc tcagcgaaaa cccaaaagaa gagctggccc gcatttacaa taaatatgta
    10441 aaccactctg actacacaaa agagcgccgt gaagatgttc tagccagaga gctaaaacga
    10501 agtatcgata gaatagatgg attgaagaac gtcttcaaac aagcaaccat tgatgggtat
    10561 ttcgcaaagt tctcaatgcc attggtcgcc aagaagcatg acaggatcca atgtgccatc
    10621 aaacctctgg cattcactca agctgaacca ggaaaaatga tggagcatag tgatacttgg
    10681 gtgatgagaa taactcgagc agcagaagaa aacctgcttt cacttgatga cattttattc
    10741 acaattgaaa ctcctgaatc accaaactca ggccaaagca aagttattga catcataaag
    10801 agaa
//
LOCUS       DAFNBJ010000163        10680 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000163 DAFNBJ010000000
VERSION     DAFNBJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.163"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            66..797
                     /gene="artJ"
                     /locus_tag="L2X49_004027"
     CDS             66..797
                     /gene="artJ"
                     /locus_tag="L2X49_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBN4799033.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     SFDIDLAKALCKQMQAECTFTNHEFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(972..2099)
                     /gene="rlmC"
                     /locus_tag="L2X49_004028"
     CDS             complement(972..2099)
                     /gene="rlmC"
                     /locus_tag="L2X49_004028"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBN4799034.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(2140..2628)
                     /locus_tag="L2X49_004029"
     CDS             complement(2140..2628)
                     /locus_tag="L2X49_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBN4799035.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2688..3533)
                     /gene="potI"
                     /locus_tag="L2X49_004030"
     CDS             complement(2688..3533)
                     /gene="potI"
                     /locus_tag="L2X49_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBN4799036.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(3530..4483)
                     /gene="potH"
                     /locus_tag="L2X49_004031"
     CDS             complement(3530..4483)
                     /gene="potH"
                     /locus_tag="L2X49_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBN4799037.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLSEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4493..5626)
                     /gene="potG"
                     /locus_tag="L2X49_004032"
     CDS             complement(4493..5626)
                     /gene="potG"
                     /locus_tag="L2X49_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBN4799038.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5721..6833)
                     /gene="potF"
                     /locus_tag="L2X49_004033"
     CDS             complement(5721..6833)
                     /gene="potF"
                     /locus_tag="L2X49_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBN4799039.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7184..7660)
                     /locus_tag="L2X49_004034"
     CDS             complement(7184..7660)
                     /locus_tag="L2X49_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBN4799040.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7748..8650)
                     /gene="rimK"
                     /locus_tag="L2X49_004035"
     CDS             complement(7748..8650)
                     /gene="rimK"
                     /locus_tag="L2X49_004035"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBN4799041.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8711..9433)
                     /gene="nfsA"
                     /locus_tag="L2X49_004036"
     CDS             complement(8711..9433)
                     /gene="nfsA"
                     /locus_tag="L2X49_004036"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBN4799042.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9417..9704)
                     /locus_tag="L2X49_004037"
     CDS             complement(9417..9704)
                     /locus_tag="L2X49_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBN4799043.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9864..10121
                     /gene="grxA"
                     /locus_tag="L2X49_004038"
     CDS             9864..10121
                     /gene="grxA"
                     /locus_tag="L2X49_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBN4799044.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10151..10528)
                     /locus_tag="L2X49_004039"
     CDS             complement(10151..10528)
                     /locus_tag="L2X49_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBN4799045.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
BASE COUNT         2803 a         2888 c         2581 g         2408 t
ORIGIN      
        1 ggcattgtta tcccatgccg cagacacggc caaaaatcat aagattgaca gacgggagtt
       61 ccatcatgaa aaagttagtt cttgccgctt tacttgcttc ctttactttc ggtgcttctg
      121 ccgcagagaa aatcaatttt ggcgtttcag ccacctatcc accctttgaa tctataggtg
      181 ctaataatga gattgtcagc tttgatatcg atctggcaaa agccttgtgc aaacaaatgc
      241 aggcagaatg tacttttact aatcacgagt tcgacagcct gatcccgtca ctgaaattca
      301 gaaaatatga cgccgtaatc tccggtatgg atatcacgcc cgagcgtagc aagcaggtat
      361 cgtttaccac gccctactat gaaaactcag ccgtcgtgat tgccaaaaaa gatacctaca
      421 aaacgtttgc cgatctgaaa ggcaaacgta ttgggatgga aaacggcacc acgcaccaga
      481 aatatattca ggatcagcac cccgaagtga aaactgtctc ctatgacagt tatcagaatg
      541 cctttatcga tctgaaaaat ggtcgtattg atggggtatt tggtgacaca gcggtggtaa
      601 acgaatggct gaaaaccaat ccgcaactgg gcgttgctac tgaaaaagtg accgatccgc
      661 aatacttcgg caccggtctg ggcatcgctg tacgtccgga taacaaagct ctgctggaaa
      721 aactgaataa cgcgctggca gcaattaaag ctgacggtac atatcagaaa atcaatgacc
      781 agtggtttcc gcagtaattg agactgatga caaaagtaag attgcctgat gcgctgcgct
      841 tatccggccc acatcaactc tgcaatgtat tgatttagca tgattttgta ggccggataa
      901 ggcgtacacg ccgcatccgg catgaacaaa gcgcacctga tcagcaatct gaagccgcac
      961 ctgcggcttt tttattgctt caccagcagc gtcagcactt catagtgcgc ggtgtgcggg
     1021 aacatatcaa aaagctgtac ccgttcgatg cggtaacctg gcagttcgcg gatatctttc
     1081 gccatggttt gggcgttaca gctggagtag ataataaaac gcggtgccat tgttgagaga
     1141 taatcacata gcggtttacc aatgccgcgg cgcggcggat taaccagcac cagctccggc
     1201 acctccccct gagcggtggc aaactgagtg gagtccagcg cctgaaattg caaacgcgtt
     1261 aagcccagtt cagcggctga ctgctttgca caggcaatgg cctctgatgc aatttcgatc
     1321 ccggttaact gcatgtcagg cgtcgcgcag tgtaaaccaa agccacccac accgcagaac
     1381 agatcccaca tatgtttaac tggcagctgc cgtacccagt cgcgcgcggt agcgtacagc
     1441 tggctggcga ccgccggatt agtctggaag aaactttgcg gacggatcca cagcggtacg
     1501 tcattaaaac gctctgccag cgcctgttgt tcagtaaggt agatctccgt ctccccttcc
     1561 ataatcgcca tatgtaccgg ctgaatattg acggtaataa ctttcagttg cggtagttgt
     1621 tcctgtaacc acggcagcgc cttacgcagt tgtgccagtt tggtttcaga acgcagtaca
     1681 aagcgcagca tcatgccgcc atcgctctgg ctttcagtca gcagaatgta tttcagttcg
     1741 ccacgcttac gcgccacgtt gtagggtgtc agccccgcac gggcgataaa cggttttagc
     1801 gccgcaaaaa cgggcgcaaa tgaggcagga taaagcgggc agtcacaaag gtcttctggt
     1861 gtgccgtcgc gatgcagcat accgagcagt ggtttttcaa cgctaccact caccaccatt
     1921 ttggctttat tacgaaaccc ttgttccggg cctgacaccg gcgcgcacca ttcctcaacc
     1981 ggaaaatcgg cgagcagatt tttaagatcg gcggttttag cggagagttg ctctggaatc
     2041 ggctgcgtta tccactgaca ggaacgacag cgacccgcgt cgtaaagtgc gcactgcata
     2101 cattgacctt cacatcatca ggggcgacga ttatacacat tattgcaact ggaagaaccg
     2161 ccgactggtc gagggaacga acagcagcat caggatgagc atatccggca gcttctgcag
     2221 catcaggcta tggaagattt cacgttttga ttcaccggga atgctgaaca gctccggata
     2281 accgtacccc agcgaagccg cccacaagta acttgcggcg gtgatttgtg tcagcagata
     2341 tagccagcgc gcccagcgac gccctttcac cagtgaaaac gcacaccaga tctcaatgaa
     2401 aaccagcacc agactgctta aaaagaccag cgttaaactc caggtttgta cgctgcgatg
     2461 aatgaactcg ccaataccgc gcacgcccag cgtattgaaa atcatcagca cgtcgaggcc
     2521 acggatcata ataatggcga gcgccgctac ctgcaccagc gcaggcacat tcaggcgagc
     2581 atgagatgaa gatgttttct taaaaaatcc caacgtttcg tcttccatga aaacaatgcc
     2641 gcggcatgcg cggcattatg tagccaggtt ggcaaatttt agtgtcttca gccatgtctt
     2701 gcacgctgga tatcgcgtat ccgctgtttt tctgcgcgag ccatcagata ccaggcgata
     2761 aatccgacaa ttccgaccgc accgagaatt aacgttgcaa gggcgttgat ttccggattc
     2821 acccccatcc gcacgctgga aaagaccagc atcggtaacg tggtggctcc cggcccggaa
     2881 acaaagctgg cgatcaccag atcatcaagc gacaaagtaa aagccagtaa ccagccagaa
     2941 atgatcgcgg gcatgatcat cggtagcgta atgacgaaaa acactttcag cggcgtcgca
     3001 ccgagatcca tcgcggcttc ttctatcgag cgatccagtt cccgcaatct tgaagaaata
     3061 acgaccgcca cataagccgt acaaaaagtg acatgcgcca gccagatggt gagcataccg
     3121 cggtccgcag gccagccaat ggcatgagca agcgcgacga ataataacaa cagcgacaag
     3181 cccgtgatga catctggcat taccagcggc gcggtgatca taaaggcaaa gccatttgat
     3241 ccgcgaaacc tgccaaagcg caccagcacc accgccgcaa tagtcccgag gatcgccgcc
     3301 gccgttgccg cacaggcagc aattgtcagg cttaaaccaa ccgcactcat catcgcgtca
     3361 tcgcgcaata actcaccata ccagcgcgtt gaccagccgg cccacaccgt caccagcttc
     3421 gagctgttaa acgaatagat aaccagcatc agcattggcg cgtagagaaa ggtaaagccc
     3481 agcagcaaaa tcacaatccg ccagggtgaa cgaactaccg gtaaattatt catccgtgtt
     3541 ctcccacgct tttttgctgg tgtttgtgga accacattat cggcacaatt agcagcagca
     3601 acatgatgat cgctaccgcc gaggccaccg gccagtcgcg gttattgaaa aactcttgcc
     3661 atagcacgcg cccgatcatg atgctgtccg ggccaccgag cagttccggg atcacaaact
     3721 cgcccacagc cgggataaac accagcatcg atccggcaat aatcccacct ttagtcagcg
     3781 gcacgatcac agtaaagaac gttttcagcg gtcgtgcacc gagatccagc gctgcttcca
     3841 ccagcgaata atcaatacga atcaacgcgg tataaatcgg cagtaccata aacggcacgt
     3901 aggcgtaaac aatgccaata taaactgcca gattggtatg aagaatggtc agcggttgat
     3961 cgataacccc cagccacagc agaaaattat tcagcacacc gttgtttttt aatattccca
     4021 tccaggcata aacgcggatc agaaacgagg tccacgacgg caggatcacc agtagtaata
     4081 aaatattacg ggtcgaaggc ttactgtgcg ccaccgccca cgccagcgga tagccgatca
     4141 gtaaacagca aaatgtcgaa atcgcagcca cctgtagcga ctggagataa gcatcgaaat
     4201 agagcggatc gtcggtcagt tgcagaaaat taccgagatt aagagtgatg gacagttgcc
     4261 cgtcagccca ctccatcagt tcggtataag gtggaatagc gcgcgccatc tccgacaggc
     4321 ttattttaaa gacaatcaga aatggcagca gaaacagcaa gatcaaccag atatatggca
     4381 acgcaatgac cagtttgcgc ccatgcttca tttgcagctg cgacaaccac agcttaaatc
     4441 cgcccggcgg tttcgactgg gcagcaggtt caagtgtatt catctttgct ccttaaaccg
     4501 tcagcaccac acagctgtcc acttcccagc acaaacgcac ttcgtcgccc caggtcggta
     4561 accctttacg atgacgatgg gcgttttgta gctgggcgct aatcatctgc ccacttttca
     4621 gacgaacgtg atacaccgaa agatcgccga gataggcaat gtgtatcacc tcccccaccg
     4681 cgaagttaca accattggcg ggcggctctt cgcaaagcat gattttttcc gggcgcagcg
     4741 ctacgtgtac cggcacgtta tcgaccactg aagcatccgc atcgactttc agtggatgca
     4801 ccagccccgg cgaatcaagc accaggccat cttcctgacg ctctttgagt acaccttcaa
     4861 agacattaac cgaaccgata aattcagcgc tgtagcgggt agttggatgc tcgtagatct
     4921 cttccggctc gccaatctgg acaaatttcc cgcgattcat aatggcgata cgccccgcca
     4981 tggtcatcgc ctcttcctga tcgtgggtga ccatcacaca agtcacaccg acgcgctcca
     5041 gaatatccac cacttcaagc tgcatccggt cacgcagctt tttatccagc gcgcccatcg
     5101 gctcatcgag cagtaatagt ttcgggcgtt tggcaaggct tcgggccagg gccacacgct
     5161 gtcgctgacc accggaaagc tgatgcggtt tgcgtttggc gaactcctgc atgtgcacca
     5221 ggccgagcat ctcatggacc cgactggcaa tttccgcttt cggtagtttg tcctgtttca
     5281 ggccaaaagc gatgttctgt tccacggtca tatgcggaaa cagcgcgtaa gactgaaaca
     5341 tcatattgat ggggcgcagg taaggcggca cctgtgacaa atcgacgcca tcaagcatta
     5401 tctgtccggc agaaggttgt tcgaaacctg ccagcatacg cagcagcgtg gacttgccac
     5461 agccggatgc gcccagcagc gcgaagattt cacctttgta gatggtcagg ctgacatcat
     5521 ccaccgcatg ttgaccatcg taggatttgg tgaggttgcg gatttctaat agcggcgtca
     5581 gcgccttacg ggttttcgcc tgcgggcgag ggatagcgtc attcacggct gctctccggc
     5641 attgaatgtg tgcaagcgca ccatgatggt gcgaattgcc ggcgggtgtg gtgcgcccgc
     5701 ccggcatcta aggctgcgga ttattttccg ctcttcactt tggtccacgc gcgggtgcgc
     5761 acacggtcga ttttcgggtc ctgcactttc agagtgaaca gcttcgcacg aacatccgca
     5821 ggcggataaa tacccgggtt ctcacggact tccgcactca ccagcggcgt ggctgcttta
     5881 ttggcgttgg catagaacac atggtcggaa atatgcgcca ccacatccgg gcgcagcagg
     5941 taattcagga actgataggc ttcgtcttta tttttggcat cggcaggcat ggcgaataca
     6001 tcaaagaatg ccatcgcccc ctctttcgga atcgagaacg agacattcac gccattcttc
     6061 gcttccttcg cgcggtttga cgcctgccag acatcgcctg cccaaccaat cgctacgcag
     6121 atgtcaccat tcgccaggtc attaatatat tgcgatgaat ggaaataacg aatgttcggg
     6181 cgcagcttta acagcagatc ggtagccggt ccggtgtaat catctgcttt agtgctgttg
     6241 ggatctttgc cgagataatt caacacggta gcaaaaactt cttctggcgc atccaggaaa
     6301 gagacaccgc agcttttcag tttttccaga ttttcaggtt tgaggatcaa gtcccagcta
     6361 tcgacgggcg cgttttcgcc cagcacagct ttaactttat caacgttata gccaatcccg
     6421 gtcgtcgccc acatataggg catagcaaat ttattgtcgg gatcgtgttt ggcgaccagc
     6481 ttcagcagct ccgggtcgag attcttccac tccggcaatt tgcttttgtc cagcggctgg
     6541 aaaactcccg cagtcaactg gcgttcaaga aagctggcag atggaaccac caagtcaaag
     6601 ccggtgctac ccgccattaa tttgccttcc agtacttcgt tagagtcgaa aacatcgtag
     6661 acgactttaa taccggtttc tttttcaaaa ttggccaccg tgtccggggc gatataatca
     6721 gaccagttat aaacgtggag tgttttttgt tcagccgcga gcgtgccgac agagacggcc
     6781 atcagagcac ccgcagccag acccgaaagc cattttttat ttaaggcggt catatctctt
     6841 tccttctgaa agttcgttaa caaacgaaca aaagttgtca cactaaaaga tatgcaataa
     6901 acgtgcatat tttttatcgg cggggtgtaa gaaaagagaa ctctctcccg gccagcactg
     6961 ctcgctatat aaaagcatcg caagaataac tttagccagg gtttgagact atagctcgga
     7021 ttaaaaaacg gtatttaaca aatttttatg cgtatttgct ctatgtgata aagccaaatc
     7081 gacacaagag aagtgaaata atctttaaga aaaggttaaa aacagcagat aattttgcag
     7141 cgcgcagcca ctgcaccagc gactgcgtgc tcgaaaggag ttatcagtgg aggaaatagt
     7201 tttcggtaac gttgttttgc ccttcatcgt cagtcggtaa cagcagttga tgggcattag
     7261 cttcaagaat gaccatcgaa atctgctctt cgctctgacg cacgaaccat gcaaactgct
     7321 cataggttac gccctgcata acggataaag actggcaaac caccagcttt ggcagattat
     7381 catcctgcat atcaagaaat gctttcacgg tcagcgaact ggcattgatg gcagataaat
     7441 ccgccgccag cggcaatacg gctgaaggtc ggacttccgc catggcagaa aacaggatcg
     7501 tgttatcgat cagatcgatt ttggcatcaa agacaccgtc gaaattctgc atatggggca
     7561 gatgcagagc ctgacagtta tcacattcaa aaaaactcat ccccaggtca tcgagccatt
     7621 gacgcagcgt atccagacca ggaacgacca gcgatgtcat aattttgtac aacctctttc
     7681 gataaaaaga ccggcacagc ttacgcaaaa agcgcaggca aaaccatgat cagtaatgtg
     7741 attgcgacta accacccgtt ttcaggcaat attcggtcgt agcgtggcgt tcgatccagc
     7801 ggatcatttt acccgcgatg tcgataccgg tggttttttc tattccttcc agccccggcg
     7861 acgcattcac ctccatcacc aacggcccgc gattagcacg cagaatatcc acaccagcaa
     7921 cgtccagcgc catcgttcgc gcggctttta tcgcgatttc acgctcctgt ggtgtgatac
     7981 tggcgacgct tgccgcgccg ccacgatgca aattggaacg aaaatcgccc tctttcgccc
     8041 gccgttcaat cgcagcgacc acttcatcgc caacaaccag acagcggata tcgcaccctt
     8101 gcgcctcttt gatatattcc tgcaccagaa tatgcgcgtt cagaccgcgg aaagcgtcta
     8161 tcacgctttc cgccgcctga cgcgtctccg ccagcacgac gccaattccc tgcgtgcctt
     8221 caaccaactt gaccaccagc ggcgcaccac cgaccatgtc gattaaatcg ctggtatcat
     8281 ccggcgaatg cgcaatgccc gtgacaggca ggtcgatgcc ctgacgcgcc agcagttgca
     8341 tggaacgcaa tttgtcacgc gcccgggcaa tggcgaccga ctcattgagc ggatagctcc
     8401 ccagcatctc gaactggcgc agtgccgccg tcccataaaa ggtaatggcg gtgccaatac
     8461 gcgggatcac tgcgtcaaaa tggggtaact tgcggccttt gtagtgaata gaagacgccg
     8521 caggatttat gttcatgtag caagaaagcg gatcaagaat ttcaaccagg tgaccgcgct
     8581 gtatagcggc ttcacgcagc cgcttacacg aatagagcgt tccatcccgg gacaatatgg
     8641 caattttcac cctgcacctc tctgtcagac ctggtcaaaa gcccgcgtat catacatcga
     8701 cgtggcggtt ttagcgcgtc gcccaaccct gtttgtgcaa ataatccaga ataaatgggc
     8761 ggctttcttt aatgattgtt cggcggatat gatcgctcca ggtatcccgg cgattattgc
     8821 tgccacgggt gaggtaatat tccgccagtt gctcgtcata ctgcgccagt gcgcctttat
     8881 ccagcggttg atagctgttt tcatgcacca aaatggaggc cggtaaacgc ggcttaagat
     8941 ccggattatc cgcaggccag ccaaggcaca gcccaaacag cggcagaaca tgctgcggta
     9001 atttaagcag tttcgtcacc gcttcaatat tattgcgcag gccgccgata tataccccgc
     9061 ccaatcccag cgattccgct gcgattaatg cattctgcgc catcattgcc gtatcaacga
     9121 caccgagcaa cagttgttcc gccaggccga gctgagcatc cggacagatc tgtaaatggc
     9181 ggttaaagtc ggcacagaac acccagaact ccgccgcttg cgctacgtgt ttttgcccgc
     9241 cggtcagcgt caccagttct tcacgtaacg ctttgtcggt aatgcgaata atgctactgc
     9301 actgcaaaaa actggaactg gacgtcgcac gggcgctgtt aataatcgcc tcacgctgcg
     9361 cttcggaaat gggttcatca gtgaaatggc gaatggagcg atggccacaa ataagttcaa
     9421 tggttggcgt cattatcttt ttctctttct gaacgtgaat attgcggtgg acggttcatc
     9481 agctgtgggg caagacgttt tgccacctga agaataacca ccaccgcagc gggaagcatg
     9541 agcaaaacac cgagaaaaat catcagaatc tgcacttctg gccgagaaaa tggctcaggc
     9601 agcgacaggg agtcgcttac cgacagcagc gccaccgcca gtagcatcat tccgataaat
     9661 tccagtatca acacgccttt aggcaattta ccgatcgcgc gcatacgctt ccctctgcaa
     9721 agtgagcctt cagtctaaaa cttttcactg tattgtgttt aacagttata gcttttagca
     9781 attaatgcaa caggttaaac ctactttcag cgaatacatt ttagcgtgat cattacaggc
     9841 ataaatctat gaggagagaa ataatgcaaa ccgttatttt tggtcgttcg ggttgccctt
     9901 actgtgtgcg tgcaaaagat ctggctgaga aattgagcaa tgaacgcgat gattttcagt
     9961 atcagtatgt agatattcgt gcggaaggga tcactaaaga agatctacaa caaaaggcag
    10021 gtaaacccgt agaaaccgtg ccgcagattt ttgtcgatca gcaacatatc ggcggctata
    10081 ccgattttgc tgcatgggtg aaagaaaatc tggacgcctg atcgtctgac aagccctcgc
    10141 gttgagggct ttactgattt tttctgtgct gtggtttaaa caaactactg ataaataaga
    10201 aacacagtgc ccccagcgca caccagaaca ccgcgcttag taaccatgcc agctcttgcc
    10261 agaatgagcg cgtcggtgaa aaaaacagcc gcataatgag catcgaacag ggtgccgcca
    10321 gcattgcgcc aaacagaggt ttcaggactt ctctacgctg tgaaaagaag ctggcgactg
    10381 ctccaggaag aatgaaaaat agcaagccga tttcaggatg cccggcagcc cgaaaagcgc
    10441 ctttcatgtg cgtcgccaga aaaaggcaca ccacaatgaa gaggacaaaa cagcagattg
    10501 cccccgccca acgttgttta tgtttcactc gttcctcctg acactgcgtc tatcgaacac
    10561 atttttcgcc agtgtggcgt tcagtaagat aaagccgctt cgcattccat gctaatatag
    10621 gccaacgcaa ttcatatagc cgttgatacc taatgtgatt acactagtaa aatatattgt
//
LOCUS       DAFNBJ010000164        10622 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000164 DAFNBJ010000000
VERSION     DAFNBJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.164"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            136..1101
                     /gene="dusB"
                     /locus_tag="L2X49_004040"
     CDS             136..1101
                     /gene="dusB"
                     /locus_tag="L2X49_004040"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBN4799046.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1127..1423
                     /gene="fis"
                     /locus_tag="L2X49_004041"
     CDS             1127..1423
                     /gene="fis"
                     /locus_tag="L2X49_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBN4799047.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1501..3774)
                     /locus_tag="L2X49_004042"
     CDS             complement(1501..3774)
                     /locus_tag="L2X49_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBN4799048.1"
                     /translation="MANKKLNATITIGGAVSGSLRGAFGTVKKNTVEIGSAISKLSRE
                     QRQLNEAMKRYGQDSAIFSRMKDRYQTVVGQVERLRSATERLKRVEDARERNLAKRQG
                     YKEGILGTVALGATVAVPIKQAMDFEDTLADIRKVANFETPDGLVKMGQAIQDMSTKM
                     PMAANEIGKIVAAGAQSGIATSELTKFAESAIKMGVAFDVSADQAGQAMAEMRAAFGM
                     TQDQMNVLADKINYLGNNTAASAKDILEITQRIGPLGSVAGAASGSIAAMGATLRGMG
                     VQNEIAATGIKNLLLSLTAGASATKSQREAFKQLGFTSKQVAKSMQLNADETIRVILS
                     KISKLPKHMQAATLSQLFGKESIGAIAPMLTQLDTYQKNLDMVKDKTKYAGSMQAEYE
                     AATAKTSAQLKIFNNRVSNLGVTIGTVLLPAFNGVLATVGPVIGKVAELAREYPTVTK
                     AVVIGSSALVALRVATFAAGFAFTFLKGGLLNVSSLFARMNASAVLASTRGLPAVATG
                     VRAIGAAFISTGVGAAVAGLALAGYQIYRHWAGVKAFFGGVGEGIKSGLEPLSDALHR
                     LYERMGPLKPVIDGVGAAVKTAFNWFTSLTEPVKYSGEELDKAGKMGMTFGKTLAAGI
                     ELVTAPITFLIDKIIWVSDNIGNLTNKALEFKNAVSDVAGGAWQKTKDFFTAPFRDDV
                     QQPAATTASGAALPSPALANRSGGTTVHSNDQYHISVKAEPGMNEDALARKVIQQLRQ
                     QQAVRQRSMMIDGAQTP"
     gene            complement(3796..4002)
                     /locus_tag="L2X49_004043"
     CDS             complement(3796..4002)
                     /locus_tag="L2X49_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799049.1"
                     /translation="MLDRIHTKPEGTNPWPELVDMVLPKVLACNTAEEMQKQRDYLTE
                     CALQLAAAALRAIGEHTLAENLLN"
     gene            complement(4012..4461)
                     /locus_tag="L2X49_004044"
     CDS             complement(4012..4461)
                     /locus_tag="L2X49_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799050.1"
                     /translation="MLEPGWWSSWTVKTTTNSTPVAPHLSPKSAHSLNHQQLLLAANT
                     MKKHDLYESMKEEARLILSETKRFQTISEKNPISYEAADLLASLRNRLNAFEHMTDEA
                     GRIMRDEAESHASALGRTKWQPSSRISSGERAKRQAVKRGAILVRSA"
     gene            complement(4544..4708)
                     /locus_tag="L2X49_004045"
     CDS             complement(4544..4708)
                     /locus_tag="L2X49_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799051.1"
                     /translation="MKRQETRQHVTVRLAPDLVRQVEREQAKLERRTGIRPSRSQVIE
                     KFLSSGLNGK"
     gene            complement(4712..5026)
                     /locus_tag="L2X49_004046"
     CDS             complement(4712..5026)
                     /locus_tag="L2X49_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799052.1"
                     /translation="MWWGSKKAAAERAALPGEILAAIEGVAMYVRHGDENTPRIVVQP
                     VGWSGFIYSDDAAEKWIRKAYPELKQYQIERSVNYLASLVRSHYREQRPKQKRESWMN
                     RY"
     gene            complement(5029..5913)
                     /locus_tag="L2X49_004047"
     CDS             complement(5029..5913)
                     /locus_tag="L2X49_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799053.1"
                     /translation="MNVLSQLINDALNHQGGHIRTDGNGDITQFIPMEPAAVRYLSMN
                     DVLTPKGGKQTTFNAVLAERSLVAQAGAEIIRIPGPMTTQPTGQTGATTGREVADRFV
                     VIRPGAFAKVTDGEEISMSGKPYMVAAFDHNTAPAYGVGWTLTRQQLKHEFADDTVLE
                     AVNTAIERGIADLADFVLLNHLESAAETLASPSFTAVAQKIAAKNLRFDEVKAIVGGD
                     CTGLELQDGVLRAYGVRAEISGQTSSTIIGAFGNAAVALDDEIRVTARRVLNGAVEIV
                     VWVNASALVPDSTMFWKA"
     gene            complement(6690..6914)
                     /locus_tag="L2X49_004048"
     CDS             complement(6690..6914)
                     /locus_tag="L2X49_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799054.1"
                     /translation="MSYRAYIIAFDPEHGTYTETEIMEGFSTEQEAVDHARNRLPEVQ
                     QELARLGENLLCSYRIRVVDSAKILPFLRS"
     gene            complement(6924..8612)
                     /locus_tag="L2X49_004049"
     CDS             complement(6924..8612)
                     /locus_tag="L2X49_004049"
                     /inference="COORDINATES: protein motif:HMM:NF017116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799055.1"
                     /translation="MRSAPNVKLLPKDPFTEAIIFAGSDAWSHAKGWEEGLGKQVAGD
                     TTPPVYLGPRQLDDLDNLRIIDDGRRSARVYLAGDIKQLQISTIADKLAVAGVKEARL
                     FKGITDREPEKWNMDRLRDAALRGESLVGMLRKEERATAAATVKDIHDMAPVLMTTWP
                     NIGSNGQPLNTRPNVERLLDNYSISAKYNEISKDVEVLVPGVSSGSDARDNCAVSEIL
                     SLAALNRLPMSNIEGHIKTIAVRNTYNPVRDFINQREWDGRSRFADLLNTISTPDDYS
                     RDLLAMLVRRWLLSAVAAAYLEAGFWSKGVLVFQGEQSLGKTAWFKALLPPDNRNLVK
                     VGATIDPGNKDSVASAISHWLVELGELDATFRKADIAKLKAFISQDRDDLRRPYDRLE
                     SKYQRRTVFFASVNPKHFLADDTGNVRWWTIPVTAVNYEHGIDTQQLWAEVLSWFEAG
                     ERWWLDRDEEAMLEVVNEQHGQTDPIEEMILARFDWNSDRLAAYIEMTATDVLLSIGQ
                     DRPTKSQATQCGNILRKLTGRDARRTSKGRFYLMPPKVFGQQDHRYFDGPNHQF"
     gene            complement(8609..8878)
                     /locus_tag="L2X49_004050"
     CDS             complement(8609..8878)
                     /locus_tag="L2X49_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799056.1"
                     /translation="MKKTTVNQAISRYNSLLRNPLRQLTVGELTAQRMAAAQLLLQAC
                     IREGVNRPWTIVSRHAAMADSLVPFRISDSESWAMYLELKRGVRK"
     gene            complement(8886..9065)
                     /locus_tag="L2X49_004051"
     CDS             complement(8886..9065)
                     /locus_tag="L2X49_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005967095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBN4799057.1"
                     /translation="MELLTAKQVTTLTTLSRMTIWRYIQAGTFPNCIKLGPKRVAWRR
                     KDVMAWLESRQPVQR"
     gene            complement(9360..10595)
                     /locus_tag="L2X49_004052"
     CDS             complement(9360..10595)
                     /locus_tag="L2X49_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN4799058.1"
                     /translation="MAENKLSDVQLRALTRKVIEKPFDVADGGSLSVRVTPSRRKILD
                     GEKRPANNIQWLFRYKNKHKSANTLTLVLGKYPALSLAEARVKRDQCKRWLAHNLDPK
                     DQFDQESAKTMKPVTIREALERWINEYAAENRVNFERHRRQFERHIYPFIGELPLEQC
                     SKARWMETFSRIKKVAPVASGYILGNCKQALIHNRRLHDVYSDALEDIKVTDVGRKQA
                     KRDRVLTSEELSGLWNELKSEYAFLPYYTALLKLLIVFGARTQEIRLSTWSEWNVKEW
                     IWTVPREHSKGGEKIIRPVPESIQPFILQLRQNHLDSGMLLGEMKKPEAVSQWGRILY
                     RRLEHSTPWTLHDLRRTFATSLNNLGIAPHVVEQLLGHSMPGVMAVYNRSQYIPEKRL
                     ALDKWIKYLEKINLLKSDN"
BASE COUNT         2460 a         2743 c         2626 g         2793 t
ORIGIN      
        1 tatcgcacca cgctcatttt aaatgcaatt ctttgatcca tctcagagga ttggtcaaag
       61 tttggccttt catctcgtgc aaaaaatgcg taatatacgc cgccttgcag tcacagtatg
      121 gtcatttctt aactcatgcg catcggacaa tatcagctca gaaatcgcct gatcgcagcg
      181 cccatggctg gcattacaga cagacctttt cggacgttgt gctacgagat gggagccgga
      241 ttgacagtat ccgagatgat gtcttctaac ccacaggttt gggaaagcga caaatctcgt
      301 ttacggatgg tgcacattga tgaacccggt attcgcaccg tgcaaattgc tggtagcgat
      361 ccgaaagaaa tggcagatgc agcacgtatt aacgtggaaa gcggtgccca gattattgat
      421 atcaatatgg gttgcccggc taaaaaagtg aatcgcaagc tcgcaggttc agcccttttg
      481 cagtacccgg atgtcgttaa atcgatcctt accgaggtcg tcaatgcagt ggacgttcct
      541 gttaccctga agattcgcac cggctgggca ccggaacacc gtaactgcga agagattgcc
      601 caactggctg aagactgtgg cattcaggct ctgaccattc atggccgtac acgcgcctgt
      661 ttgttcaatg gagaagctga gtacgacagt attcgggcag ttaagcagaa agtttccatt
      721 ccggttatcg cgaatggcga cattactgac ccgcttaaag ccagagctgt gctcgactat
      781 acaggggcgg atgccctgat gataggccgc gcagctcagg gaagaccctg gatctttcgg
      841 gaaatccagc attatctgga cactggggag ttgctgcccc cgctgccttt ggcagaggtt
      901 aagcgcttgc tttgcgcgca cgttcgggaa ctgcatgact tttatggtcc ggcaaaaggg
      961 taccgaattg cacgtaaaca cgtttcctgg tatctccagg aacacgctcc aaatgaccag
     1021 tttcggcgca cattcaacgc cattgaggat gccagcgaac agctggaggc gttggaggca
     1081 tacttcgaaa attttgcgta aacagaaata aagagctgac agaactatgt tcgaacaacg
     1141 cgtaaattct gacgtactga ccgtttctac cgttaactct caggatcagg taacccaaaa
     1201 acccctgcgt gactcggtta aacaggcact gaagaactat tttgctcaac tgaatggtca
     1261 ggatgtgaat gacctctatg agctggtact ggctgaagta gaacagcccc tgttggacat
     1321 ggtgatgcaa tacacccgtg gtaaccagac ccgtgctgcg ctgatgatgg gcatcaaccg
     1381 tggtacgctg cgtaaaaaat tgaaaaaata cggcatgaac taaagtcaag ctgtaacggc
     1441 atgaaattaa aggggctatt tcagcccctt tactttttgt tgcaaccgta ctgacaaccc
     1501 ttatggtgtt tgcgcgccat ctatcatcat gcttcgctgc cttactgctt gttgctgtcg
     1561 tagttgctgt atcaccttcc gggctaatgc atcttcattc ataccaggtt ctgccttcac
     1621 gctgatgtgg tactggtcgt tgctatgcac cgtagtgcca ccagagcggt tggccagtgc
     1681 aggtgacggc agggcagcac cagaggcagt ggttgctgct ggctgctgta catcgtcgcg
     1741 gaatggtgcg gtaaagaaat ctttggtttt ctgccacgca ccaccagcaa catcagacac
     1801 ggcgttttta aactccagtg ccttgttggt caggttgcca atgttgtcag aaacccaaat
     1861 gatcttatca atcaggaacg tgatcggtgc ggtcacaagc tcgatgccag ccgccagggt
     1921 cttaccgaac gtcatcccca ttttaccggc cttatccagt tcctcgccgc tgtatttgac
     1981 cggttcggtc agtgacgtga accagttgaa agccgtctta accgcagcgc ccacgccatc
     2041 aatgacgggc ttcaatggcc ccatgcgctc atacaggcga tgcagggcat cgctaagcgg
     2101 ttccagcccg gatttgatac cttcacccac gccaccaaaa aaagccttca caccagccca
     2161 atggcggtaa atctgataac ctgccagcgc caggccagct acggcagcac caacgccagt
     2221 ggaaatgaac gccgcaccga tggcgcgcac gccggtagcc accgcaggca ggccacgagt
     2281 gctggccaga acggcggatg cgttcatgcg ggcaaacagg ctggacacgt tcagcaatcc
     2341 gcctttcagg aacgtgaacg caaagccagc ggcgaacgtc gccacacgca gtgccaccag
     2401 tgcactggaa ccaataacca ctgctttggt cacggtaggg tattcgcgcg ccagttcggc
     2461 aaccttgccg ataaccgggc caacggtagc cagaacgccg ttgaatgctg gcagcagcac
     2521 tgtgccgatc gtgacgccca ggttagacac acggttgttg aaaatcttaa gctgcgccga
     2581 cgttttggcg gtggctgcct catattcagc ctgcatagaa ccggcatatt tggtcttgtc
     2641 tttgaccata tccagattct tttggtacgt atcaagctgt gtcagcattg gtgcgatagc
     2701 gccaatcgat tccttaccaa acagttgcga aagcgttgcc gcctgcatgt gttttggcag
     2761 cttgctgatc ttggacagga tgacgcggat tgtttcatcg gcgttcagtt gcattgattt
     2821 tgcaacctgc ttggatgtga agccaagctg tttaaacgct tcacgctgtg acttcgtggc
     2881 ggatgcgcca gcggtcaggg atagcagcag gtttttaata ccggtggccg caatctcatt
     2941 ctgcacgccc ataccgcgca gcgttgcacc catcgcagcg atagaaccgg aagctgcacc
     3001 agcgacgctg ccaagtggcc cgatgcgctg cgtgatttcc aggatgtctt tggctgatgc
     3061 ggcggtgttg ttgcccaggt agttgatctt gtcggccaga acgttcatct ggtcctgcgt
     3121 catcccgaag gcggcgcgca tctctgccat tgcctggcct gcctggtcgg cgctgacgtc
     3181 aaaagccaca cccattttga tggccgattc agcaaacttg gttagttcgc tggtggcgat
     3241 gcctgattga gcaccagcgg caacaatctt gccgatttcg ttggctgcca tcggcatttt
     3301 ggttgacata tcctggatag cctggcccat cttaaccagg ccgtcagggg tttcgaagtt
     3361 ggcaaccttg cggatatctg ccagcgtatc ttcaaaatcc atagcctgtt tgatcggtac
     3421 agctaccgtt gcgccaagtg cgacagtccc caggattcct tctttgtagc cttgacgctt
     3481 cgccaggttg cgttcgcggg catcctccac gcgtttcagg cgttcggtgg cagaacgaag
     3541 gcgttcaacc tgcccgacaa ccgtttgata gcggtccttc atgcgggaga atatcgcgct
     3601 atcctggcca taacgcttca ttgcttcatt taactggcgc tgttcgcgcg acagcttaga
     3661 gattgcgcta ccaatctcga cggtattttt ctttacagtt ccaaatgcac cacgaaggct
     3721 gccggaaacg gccccaccaa tcgtgattgt tgcatttaac ttcttgttgg ccataataat
     3781 tgtgctctca aggaattaat taagtaaatt ttctgcaagg gtgtgttcac cgattgcccg
     3841 caaggcggca gcggccagtt gcagggcgca ttcggtgagg tagtctcgct gtttctgcat
     3901 ttcctccgct gtgttgcacg ccagaacttt tggcaggacc atatccacca gttcaggcca
     3961 ggggtttgtt ccttccggtt ttgtgtgaat acggtccagc atggtgcccc cttatgcact
     4021 ccgtaccagg atagcgccgc gcttcaccgc ctggcgtttg gcccgttcac cggaactgat
     4081 gcggctggat ggttgccact tcgtgcgtcc cagggcggat gcgtggcttt ccgcttcgtc
     4141 acgcatgatg cggcccgctt catcggtcat gtgttcgaat gcgtttaaac ggttgcgaag
     4201 gcttgccagc aggtcggctg cttcgtagct gatagggttt ttctctgaaa tggtttggaa
     4261 acgctttgtt tcgctaagga tcaaacgcgc ttcttctttc attgattcgt acaggtcatg
     4321 ttttttcata gtattggccg ctaacaatag ttgttgatga ttaagtgagt gcgcgctttt
     4381 aggtgacagg tgcggcgcaa ccggtgtgga attagtggtg gttttgaccg tccaggatga
     4441 ccaccaacct ggttccagca tccatcgtct gaggaacgat gaaggttaaa tttggccggt
     4501 tatttggggc acagggaacc ggtgaaacct gtattcttgg ccattatttg ccgttcagcc
     4561 cgctactcag aaacttttca atcacctggc tgcgtgaagg gcggatgcct gttctgcgtt
     4621 ccagtttggc ttgctctctt tcgacctggc gcaccaggtc aggggcgagg cgtaccgtta
     4681 cgtgctggcg tgtttcttgt cgtttcatgg cttagtacct gttcatccag ctttcgcgct
     4741 tctgtttcgg gcgctgctcg cggtagtgac tgcgcaccag ggaagcgagg taattcacgg
     4801 agcgttcaat ctggtactgc ttaagctccg ggtaagcctt gcgaatccat ttttcagcag
     4861 cgtcatcgct gtagatgaat cctgaccagc caaccggctg cactacgatg cgcggtgtgt
     4921 tttcatcacc gtggcgcaca tacatggcca caccttcgat agctgccagg atttcacccg
     4981 gcagggcagc gcgctccgct gccgcttttt tgctgcccca ccacatgatc aagccttcca
     5041 gaacatagtt gagtcaggca ccagtgcgga tgcgttcacc cacacaacga tttcaacggc
     5101 accattcagc acgcggcggg cggtgacgcg gatttcatca tccagggcaa cggctgcatt
     5161 gccgaaagcg ccaatgattg tgctggaagt ctggccactg atttcagcgc gtacaccgta
     5221 ggcacgcaga acgccgtctt gcagttccag gccggtgcaa tcaccgccaa caatggcttt
     5281 aacttcatca aagcgcaggt ttttggctgc aatcttctgc gccactgcgg tgaatgacgg
     5341 gctggccagc gtttccgctg cgctttccag gtgattcagc agcacgaagt cagcaaggtc
     5401 agctatgccg cgctcaatgg cggtgttgac ggcttccagc acggtatcgt cagcgaattc
     5461 gtgtttcagt tgctggcggg tcagggtcca gcccacgccg taagccggtg cggtgttgtg
     5521 gtcgaatgct gcaaccatgt acggcttgcc gctcatgctg atttcttcgc cgtcagtaac
     5581 tttggcgaac gcaccagggc ggatgacaac gaagcggtca gccacttcgc ggccagtcgt
     5641 tgcaccagtc tggccagtcg gctgcgtggt catcgggcca ggaatgcgga tgatttcagc
     5701 gcctgcctgt gccaccagcg aacgttcagc aagaacagcg ttgaaggtag tctgcttgcc
     5761 gcctttcggg gtcagcacgt cattcatgga caggtagcga actgctgccg gttccatagg
     5821 gatgaattga gtaatgtcac cgttgccatc agtgcgaatg tggccgccct ggtggttcaa
     5881 cgcgtcgttg ataagttgag ataaaacgtt cattaaaaat gctccggttg ttgtgcaata
     5941 aatcgtctgg aatgacttaa agatatacag taaccagacc gaacacaata gccacaatgt
     6001 gagcttttta atgtaaaatc agtaacttat aaccatgaat acttgcaaat aaccaggcat
     6061 aatccggcgt aatcgatatt tacatatctt tacaattcag cgtgtgtaaa acgtgttcgc
     6121 tttattttgc aatcgcgcac gtatggtgcc agtgctttga taggattcac ataaatgctt
     6181 gatttacttg acgtaatcat aaagtgagtt ttggaggggc acgcacaacg caaattacgc
     6241 gggaccaatg cccccgcagg ctgtaccccc ctcggaagta ccttgacttt tttggcagca
     6301 gatggcgatg acacctcatg tcaccatgac ggcgttatgt tggaaaggtg tcattaggtg
     6361 tcatagcggg aaacacgctg attattaaag aaaaatacaa acaagatact gtaatgaatg
     6421 acacctgtga caccatgaca cccatttcta taaagtagaa cgaccactgt tacattatta
     6481 cagtggtcgt tattttcttt aaaaatgctg tcataggtgt cataggtgtc ataggtgtca
     6541 ttagtaatgc cgcaaccctt gcagattagg gcttgagagc aatgacacct accatgacac
     6601 cttataaatt aatgacacct aggcgtcact ttggaaataa aaaaaagccc cggcattcgc
     6661 cagggcaatg ggcaacaact gttgtgatgt tatgatcgga gaaacggcaa gattttagcg
     6721 gaatccacta cccttatgcg gtagctgcat agcaggtttt cacccaacct ggccagttcc
     6781 tgctgcacct ctggcagcct attgcgagca tggtctaccg cttcctgttc agtggagaaa
     6841 ccctccataa tttcggtttc ggtgtaggtg ccgtgctccg ggtcaaaagc aatgatatat
     6901 gcgcgataac tcatagtatt acctcaaaac tgatggttag gcccatcaaa atagcgatgg
     6961 tcttgctgtc caaaaacttt cggtggcatt aggtaaaaac gccctttact tgttctacgt
     7021 gcatcccttc ctgtcagttt tcgcagaatg ttaccgcact gcgttgcctg ggattttgtt
     7081 ggccgatcct gcccaataga caacagaaca tccgttgcag tcatttctat atatgcagcc
     7141 aggcgatcac tgttccaatc aaaacgcgcc aatatcattt cttcaatcgg gtcagtttgc
     7201 ccgtgctgtt cgttcacgac ttccagcatg gcttcttcat cacggtccag ccaccagcgt
     7261 tcacctgctt caaaccagga aagaacttcg gcccataatt gttgtgtgtc tattccatgc
     7321 tcgtagttaa cggcagttac aggaatggtc caccagcgaa cgttccctgt gtcatccgcc
     7381 aggaagtgtt tcgggttcac tgacgcaaaa aacacggtgc ggcgctggta cttcgattca
     7441 agccggtcat aagggcggcg caggtcatca cggtcctggc tgataaatgc tttgagcttg
     7501 gcaatatccg ccttgcgaaa cgtcgcatcc aattcgccta gctcaacaag ccagtgacta
     7561 attgcgctgg ccacgctgtc tttattgccg gggtcaatag ttgctcccac cttaaccagg
     7621 ttgcgattgt cagggggcag aagcgcttta aaccatgctg ttttacccag tgactgctcg
     7681 ccctggaaaa ccagcacgcc tttggaccag aaacccgctt ctaagtaagc agccgccacg
     7741 gcagacagca accaacgacg caccagcatg gccagcagat cgcgactgta gtcatctggc
     7801 gtgctgatag tattcagcag gtctgcaaat cggcttcgtc catcccattc acgttgattg
     7861 atgaaatccc tgacaggatt ataagtattg cgcacagcga tagttttgat atgaccttca
     7921 atattactca ttggtaaccg gttcagtgcg gcgaggctta aaatttcact cacagcgcag
     7981 ttatcgcgag catctgaacc gctactaacg ccaggtacta aaacctcaac gtccttgcta
     8041 atctcattat attttgcaga aatgctgtag ttatctaata gacgttcaac attagggcgt
     8101 gtgtttaaag gctggccatt gcttccaata tttggccaag ttgtcattaa tacaggagcc
     8161 atatcgtgaa tgtcttttac agttgctgcg gcggttgctc gttcttcttt gcgcagcatg
     8221 cccaccaggc tttcaccgcg tagggcggcg tctctaagcc tgtccatgtt ccatttttcc
     8281 ggttcacggt cagtaatgcc tttaaataaa cgggcttcct taactccagc cactgccagc
     8341 ttgtcagcaa tagtgctgat ttgaagttgc ttaatatcac cagccagata tacccgcgca
     8401 gaccggcgac catcgtcgat aattcgtaga ttatctaaat catccagttg tcttggccca
     8461 agatagacag gaggtgtggt atcaccggcc acctgtttgc ccaaaccttc ttcccaaccc
     8521 ttggcgtgac tccaggcgtc agaaccagca aaaataattg cttccgtaaa agggtctttc
     8581 ggtaacagct taacgttggg tgcgctcctc attttcgcac cccgcgtttt aactccagat
     8641 acatcgccca tgattcagaa tcactgatac ggaacggcac caagctatca gccatagcgg
     8701 cgtgacgtga aacaatagtc cacgggcggt ttacaccttc gcgaatgcat gcttgcaaca
     8761 ataattgtgc cgctgccata cgctgtgcag tgagttcgcc aacggttaac tggcgcaggg
     8821 gattacgcag caggctgtta tagcgactga tagcctggtt tacggttgtt tttttcatag
     8881 ccacctcagc gctgaactgg ctggcggctt tcaagccatg ccataacatc tttacgccgc
     8941 caggccacac gttttggccc taatttaatg caattaggaa acgtgccagc ctggatatag
     9001 cgccagatag tcatgcggga taatgttgtt agtgttgtga cttgcttggc agtcagtaat
     9061 tccatcttgc ggccctcgaa acatggccgg tggatatatg gtctgcaatg atttatgatt
     9121 atgttgcgtt atcaaagaac gcaaccatta cagccttcac cggctttttg gtattaaggg
     9181 gttcggtttt ctgtttggcg acgtgaaccg atccccgcct tattcaagcc ctcagttctg
     9241 ctgggggctt tttattacta catgacaata atactcaaat tgtatcagca gtaaataccc
     9301 tttaaattca taaacttaac tgcaaataac gctacataac tctatataat catcaatttt
     9361 taattatctg attttaaaag gtttattttt tctaaatatt ttatccattt gtccaaggct
     9421 aaccgttttt ccgggatgta ctggctacgg ttgtacaccg ccatcactcc tggcattgag
     9481 tggcccagca actgttcaac cacatgcggg gctatcccca agttatttag actggtggca
     9541 aaagtgcggc gcaggtcatg caacgtccac ggcgttgaat gctctaatcg tctatataag
     9601 atacgccccc actggctgac agcttcgggt ttcttcattt caccaagcag cattccgcta
     9661 tcaagatgat tttggcgtag ctgtaaaata aatggctgta tagattcagg cacgggtctg
     9721 atgattttct ccccaccctt gctatgttct cgcggaactg tccaaatcca ttctttcacg
     9781 ttccattccg accatgtcga aagacgaatt tcttgtgttc ttgcaccaaa cactattagc
     9841 aattttaaaa gcgcggtgta gtaaggtaga aacgcatatt cgctttttag ctcattccaa
     9901 aggccactaa gttcttcaga agttaaaaca cgatcacgtt ttgcctgctt tcttccaaca
     9961 tcagtaactt tgatatcctc cagcgcatca ctgtaaacat cgtgcagcct gcggttgtga
    10021 atcaacgcct gcttgcagtt acctaatatg tagccgcttg ctaccggcgc gacttttttg
    10081 attctgctaa atgtctccat ccatcttgct ttagagcact gttcaagtgg caattcgccg
    10141 atgaaaggat aaatatgtct ttcgaattgg cgtctatgcc tttcaaagtt gacccggttt
    10201 tccgccgcgt actcattaat ccagcgttcc aatgcttccc tgatagtgac aggtttcatg
    10261 gtttttgctg actcttggtc aaactggtct ttaggatcta gattatgagc aagccaccgt
    10321 ttgcattggt cgcgtttcac cctcgcctca gcaagagata aggcaggata cttgcccagc
    10381 actagcgtca aagtattggc gcttttatgc ttattcttat aacggaacaa ccattggatg
    10441 ttattggccg gtctcttctc gccatcaagg attttgcgcc ttgatggtgt aacgcgaaca
    10501 gaaaggctgc caccgtcagc aacatcaaaa ggtttttcga tgactttgcg ggttaacgca
    10561 cgcaattgaa catctgagag tttattttcg gccatagagt taatgacaac ctttttgaca
    10621 ac
//
LOCUS       DAFNBJ010000165        10459 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000165 DAFNBJ010000000
VERSION     DAFNBJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.165"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            46..1413
                     /gene="dcuC"
                     /locus_tag="L2X49_004053"
     CDS             46..1413
                     /gene="dcuC"
                     /locus_tag="L2X49_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBN4799059.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1456..1953)
                     /gene="sspB"
                     /locus_tag="L2X49_004054"
     CDS             complement(1456..1953)
                     /gene="sspB"
                     /locus_tag="L2X49_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBN4799060.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1959..2597)
                     /gene="sspA"
                     /locus_tag="L2X49_004055"
     CDS             complement(1959..2597)
                     /gene="sspA"
                     /locus_tag="L2X49_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBN4799061.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2992..3384)
                     /gene="rpsI"
                     /locus_tag="L2X49_004056"
     CDS             complement(2992..3384)
                     /gene="rpsI"
                     /locus_tag="L2X49_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBN4799062.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3400..3828)
                     /gene="rplM"
                     /locus_tag="L2X49_004057"
     CDS             complement(3400..3828)
                     /gene="rplM"
                     /locus_tag="L2X49_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBN4799063.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4047..5174)
                     /gene="zapE"
                     /locus_tag="L2X49_004058"
     CDS             complement(4047..5174)
                     /gene="zapE"
                     /locus_tag="L2X49_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBN4799064.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMMRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5368..5766
                     /locus_tag="L2X49_004059"
     CDS             5368..5766
                     /locus_tag="L2X49_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBN4799065.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5920..7287
                     /gene="degQ"
                     /locus_tag="L2X49_004060"
     CDS             5920..7287
                     /gene="degQ"
                     /locus_tag="L2X49_004060"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBN4799066.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7377..8444
                     /gene="degS"
                     /locus_tag="L2X49_004061"
     CDS             7377..8444
                     /gene="degS"
                     /locus_tag="L2X49_004061"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBN4799067.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8506..9444)
                     /gene="mdh"
                     /locus_tag="L2X49_004062"
     CDS             complement(8506..9444)
                     /gene="mdh"
                     /locus_tag="L2X49_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBN4799068.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGKVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9879..10349
                     /gene="argR"
                     /locus_tag="L2X49_004063"
     CDS             9879..10349
                     /gene="argR"
                     /locus_tag="L2X49_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBN4799069.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2547 a         2665 c         2628 g         2619 t
ORIGIN      
        1 gccgtttatg ggattgaccg ttttcttttg acacggagtt caacaatgtt cggcataatt
       61 atatctgtca tcgtattaat tacgatgggc tatttgatcc tgaaaaacta caaacctcag
      121 gtggtgctgg ctgccgcagg tatcttcctg atgatgtgcg gtgtctggtt agggttcggt
      181 ggtgtactcg atcccgccaa aagcagcggc tacttgatcg tcgatattta taatgaaatc
      241 ctgcgcatgc tgtccaaccg cattgccgga ttggggctat cgattatggc ggtgggcggt
      301 tatgcccgct acatggagcg cataggggcc agtcgcgcga tggtgagctt gttaagccgc
      361 ccgttaaaac tcattcgctc gccgtatatt attctgtcgg caacttacgt catcggtcaa
      421 atcatggcgc agtttattac cagcgcctcc ggtctgggta tgttgctgat ggtcacctta
      481 tttccgacac tggtgagtct gggagtaagt cgtctctctg cggtggcggt tatcgcaacc
      541 acaatgtcca ttgagtgggg aattctggaa acgaactcca tttttgcagc ccaggtcgcg
      601 ggaatgaaaa ttgccactta cttcttccac taccagcttc cggtcgcctc ttgcgtcatt
      661 atctcggtgg cgatctccca ctttttcgtg caacgcgctt ttgacaaaaa agataaaaat
      721 atcaatcacg aacaagcaga gcaaaaagct ctcgataatg tcccgccgct ctattacgcc
      781 attttacctg tgatgccgtt aatcctgatg ctcggctcgc tgttcctcgc ccacgtcggg
      841 ctgatgcagt cagaactgca tctggtggtg gtgatgttac tgagtttgac ggtgacgatg
      901 tttgttgagt tcttccgcaa gcataacttg cgcgaaacaa tggacgatgt gcaggcgttt
      961 tttgacggca tgggtacgca gtttgccaac gtggtaacgc tggtggtcgc gggtgaaata
     1021 tttgcgaaag gcttaacgac gattggcact gttgatgcgg ttatcagggg tgcggagcat
     1081 tctggtctgg gcggtattgg cgtgatgatt attatggcgc tagtcattgc catttgtgcc
     1141 attgtgatgg gctctggcaa tgcgccgttt atgtcatttg ccagtcttat tccgaatatc
     1201 gcagccggac tacatgtacc agcggttgta atgattatgc cgatgcattt tgccacgacg
     1261 ctagcgcgcg cggtttcgcc gattactgcg gtggtggtcg ttacgtcagg aattgcaggc
     1321 gtttcgcctt ttgcggtggt gaagcgaaca gcgatcccca tggcagtcgg tttcgtggtg
     1381 aatatgattg ccacaatcac gctattttat taagccatta aaagacaaaa caggccacca
     1441 gggcctgttt ttgtattact tcacaacgcg taatgccggt cgaccaccgc gtggtggctg
     1501 cggaggttca tcgtcaggat gagtgtcatc atcgtgatct ggcttgtcgc catcaataac
     1561 cgacataacg gtttcgttgt ctgccgatgc ctcttcatca ttcatgatgc tggtatcttc
     1621 atcgtaggca gcttcaggct caaacattgt gcctgcgcca ttttcacggg cgtagatagc
     1681 cagcacggca gccagcggca cagaaacctg acgcggaatg ccgccaaagc gcgcgttaaa
     1741 gcgcacctca tcattcgcca gttccagatt gccgacagca cgcggcgcaa tgttgagtac
     1801 gatttgcccg tcacgcgcat attccatagg aacctgcacg ccagggagcg tcacatccac
     1861 caccaggtgc ggcgtgagct ggttatccag caaccactca tagaatgcac gcagcagata
     1921 gggacgacgt ggtgttagct gtgacaaatc catacagatt aactccggcc cagacgcatt
     1981 tcacgttctg cttcagttaa agaagcaagg aaagagtcac gctcaaagac gcgggtcata
     2041 tagcctttca gctctttcgc acccgggccg ctgaactcga tgcccagttg cggcagacgc
     2101 cacagcagcg gagcaagata gcaatcgacc aggctgaact catcgctcag gaagtacggc
     2161 ttctgaccga agaccggcgc aatcgccagc agttcttcgc gcagttgctt acgtgcggca
     2221 tctgcttcag aagctgaacc gttgatgatg gtgttcatca gcgtgtacca gtctttttcg
     2281 atacgatgca tgtacagacg gctttcaccg cgagctaccg ggtaaacagg catcagtggc
     2341 ggatgcggga aacgctcatc cagatattcc ataatgatgc gagattccca cagggtcagc
     2401 tcacgatcca ccagggtcgg aacgctctga ttcgggttga ggtcaatcag atcctgaggc
     2461 ggattgtcct tttccacgtg ttcgatctcg aaacttacac ctttctcagc cagcacaatg
     2521 cggacctgat ggctatagat gtcagtagga ccggaaaaca gcgtcattac cgaacgtttg
     2581 ttggcagcga cagccatgaa aacctccagg tatagtcaga atttttactg ctaccagcca
     2641 ccaggtggcc agtcagaagt tgtgttaccc agtaaggaac gactctcttt gttcgaaaat
     2701 caaacaaaaa atgagcaata cccgacattt gggcagaaaa ttggatgata gtttaccaga
     2761 ttttgcgacc tttgtggtga gtcgatgccg gaaatgggga aaaagagatg cgctttagtc
     2821 tgaaatagtt gacttagtcc cttattggcg atgtggtttt tgttttgcct gtctgtcaga
     2881 cggtagcaaa aagcaacttt ccaattttta cgctgattca gaatttaacc ataaaaaaac
     2941 ccgccgaagc gggttttttc gcaaattgtt ttctgccgga gcagaagcca attaacgttt
     3001 ggagaactgc ggacgacgac gtgctttacg cagaccgact ttcttacgtt caacctgacg
     3061 agcgtcacga gtaacgaagc cagctttacg cagttcagaa cgcagggact cgtcgtattc
     3121 catcagagcg cgggtgatac cgtgacggat cgcaccagcc tgaccagaga taccaccacc
     3181 tttaacggtg atgtacaggt ccagtttctc aaccatgtcg accagttcca gcggctgacg
     3241 aactaccatg cgagcagttt cacgaccgaa gtactgttcc agagaacgtt ggttgattac
     3301 gattttaccg ttgcccggtt tgatgaaaac gcgagctgcg gaacttttgc ggcgaccagt
     3361 gccgtagtat tgattttcag ccattgccta taatcccgat tagatgtcaa gaacttgcgg
     3421 ttgctgtgcc gcgtggttgt gctcgttacc cgcgtaaact ttcagtttac ggaacatagc
     3481 acgacccagc gggccttttg gcaacatgcc tttaaccgcg atttcaatca cacgctcagg
     3541 acggcgagca atcatctctt caaaggtcgc ttgtttgata ccaccgatgt ggccggtgtg
     3601 gtgatagtac actttgtcag tacgcttgtt gccggttaca gcaactttgt cagcgttcag
     3661 aacgatgatg taatcaccgg tatctacgtg cggagtgtat tccgctttgt gcttaccgcg
     3721 caggcgacga gccagttcag tagccagacg gcccagagtt ttaccggtcg cgtcaacaac
     3781 ataccagtcg cgttttacgg tttctggttt agctgtaaaa gttttcatta aaagcttacc
     3841 caataaatag ttacacgttg gtgaacaccc aaacgtcttc aattgttgag gttcacacga
     3901 caaagtccgg caaacctacc ccttcgaata gcctatgcca gcacacaaaa agttttggga
     3961 aaaaaacttt cttgtaacgt ggggtcgcag gattatagag aagtcggggt caaagatcga
     4021 cccctttttg tgatttgtga caggttttaa cccgccaaat gctcgcgctt cagatactct
     4081 tcgctttgca tctcttgcag acgtgacagg caacgctgga actcaaactt cagccgatcg
     4141 ccctgataaa tttcatacag cggcacttct gcactcacca ctaatttgac atggcgctcg
     4201 taaaactcat ccaccagcgc aataaagcgc cgcgcttcgc tctccatcaa ccgcgtcata
     4261 actggtacat caaacaacat gaccgtatga aagagacgtg agagcgcaat atagtcatgc
     4321 tgactgcggg cgtcgacgca cagcgtagta aaagagaccg ccagcgtctg gttctcgacg
     4381 cccattgtcg ccaatggccg atggttgatt tctaacgtcg gtgaattttc tcgtttcgcc
     4441 cccgccagcg ccaaccatag tttatccatc tgcgcccggg tttcatcgtt gagtggcgaa
     4501 agccacagat gcgcctgagt gagtgtacgc agtcgataat caacaccagc gtccacgttc
     4561 attacatcac aatgctgttt aatggcatcg attgcaggca gaaaacgcgc acgttgcagg
     4621 ccatttcgat aaagttcatc cggcggaata tttgacgtcg ctaccagggt aatgccgcgg
     4681 gcgaacaggg ctttcatcag accgccaagt agcatggcgt cggtaatatc agaaacaaaa
     4741 aattcgtcaa aacagagcac gtcagtttcg gctttaaagc gatcggcaat aatttccagc
     4801 ggatcggtct gcccctgtaa ggcagttagc tcttcatgca cccgcatcat aaaacggtga
     4861 aagtgcaggc gctgtttccg ctctcccggc aggctttgat agaaaaggtc catcagccag
     4921 gttttcccgc gtcctacacc gccccacata tataagccac gcactggcgt atgctttgtg
     4981 tcttcgcgtt taccccacag cttaccgacc cgcgccatta gcccactcgt cctgggggct
     5041 ggtggcgtgc tattgatgag ttcctgataa ataatttcca ggcggctgac ggcctctttt
     5101 tgaacgtcgt cgggttgatg gctgccttca ttgagcgcct tcaggtattg cgatgttggg
     5161 gtaacgcttt gcatgatctt attgttattc cttgaataat cggtgcgccg ttgttcacgg
     5221 ttgacgaaaa aaaggccgtt ctacactacg cgatatgcag tcgggattcc acttctgtgg
     5281 aattaacggt tatagtggca taatcagccg caggcatgga gcctgaagcc aacaccctac
     5341 ggaaacaaaa gacaacggga gatgttcatg acctgggaat atgcgctaat tgggttagtc
     5401 gtcggcatca ttattggtgc tgtggccatg cgttttggta atcgtaaact acgccagcaa
     5461 caggcgttgc agtacgaact ggaaaagaat aaagctgaac tggacgagta tcgcgaagag
     5521 ctggttagcc actttgcccg cagcgcggaa ttactggata ccatggcgca cgactatcgc
     5581 cagctgtatc agcacatggc gaaaagctcc agcagcctgc tgccggaact gtctgctgaa
     5641 gcaaacccgt tccgtaatcg tctggcagag tctgaagcca gcaacgatca ggcaccggtg
     5701 cagatgcctc gcgactattc tgaaggcgca tccggcctgc tgcgtactgg cgcgaagcgc
     5761 gactaattta tttttcgggc gcagccattg cgccctcctc ttctctccct ccccgactat
     5821 catttaatct ggtgtctcat tgttagccgt ctgaaaattc aataacatca aactgttttg
     5881 aatctctttt cttatcattc aggtacgaga gcaggaataa tgaaaaaaca aacccagctg
     5941 ttgagtgcat tagcgttaag tgtcgggtta actctctcgg cgtcatttca ggccgtcgcg
     6001 tcgattccag gccaggttgc cgatcaggcc cctctcccca gtctggctcc aatgctggaa
     6061 aaagtgcttc cggcagtggt gagcgtacgg gtggaaggaa cggccagtca gggacagaaa
     6121 atcccggaag aattcaaaaa gttttttggt gatgatttac cggatcaacc tgcacaaccc
     6181 ttcgaaggtt taggttccgg tgtcatcatc aacgccagta aaggctatgt gctgaccaac
     6241 aaccatgtga ttaatcaggc acagaaaatc agtattcagc tcaatgatgg gcgcgagttt
     6301 gatgcaaaac tgattggtag cgatgaccag agcgatatcg ccctgttaca aattcaaaac
     6361 ccgagcaaat taacgcaaat cgctattgcc gactccgata aattgcgcgt cggtgatttt
     6421 gccgtagcgg tcggtaaccc atttggcctt gggcaaaccg ccacctctgg cattgtttcc
     6481 gcattaggac gcagcgggtt gaatcttgaa ggtctggaaa actttatcca gacagatgct
     6541 tccattaacc gcggtaactc cggcggtgca ctgttaaacc ttaacggtga gttaattggc
     6601 atcaacactg caatccttgc gcctggcggc gggagcgtcg ggattggatt tgccatcccc
     6661 agtaatatgg cgcgaacact ggcgcagcag cttatcgact ttggtgaaat caaacgcggt
     6721 ttgttaggca tcaaaggcac cgagatgagt gccgatatcg ccaaagcctt caaccttgac
     6781 gtgcagcgtg gcgcgtttgt cagcgaagtg ttgccaggtt ctggctcagc gaaagcgggc
     6841 gtcaaagcgg gcgatattat taccagcctc aacggcaaac cgctgaatag ctttgctgag
     6901 ttgcgctctc gtatcgcgac caccgagccg ggcacgaaag tgaagcttgg cctgctgcgt
     6961 aacggcaagc cactggaagt agaagtgacg ctcgatacca gcacctcttc gtcggccagc
     7021 gctgaaatga tcacgccagc gctggaaggt gcaacgttga gcgatggtca gctaaaagat
     7081 ggcggcaaag gtattaagat cgatgaagtt gtcaaaggaa gcccagctgc tcaggctggc
     7141 ttgcaaaaag acgatgtgat cattggcgtc aaccgcgatc gggtgaactc gattgctgaa
     7201 atgcgtaaag tgctggcggc aaaaccggcc atcatcgccc tgcaaattgt acgcggcaat
     7261 gaaagcatct atctgctgat gcgttaatgt cgtaaaccgg gcatcaggct tacgtgtgat
     7321 gtccggttaa ctcgtggtat gctgctgccg ttcccttttt taatgacgcc tccatcatgt
     7381 ttgtgaagct cttacgttcc gttgcgattg gattaattgt cggcgctatt ctgctggttg
     7441 ccatgccttc gctgcgcagc cttaacccgc tttccactcc gcaatttgac agtaccgatg
     7501 agacgcctgc cagctataat ctggcggttc gccgcgccgc gccagcggtg gttaacgttt
     7561 acaaccgtgg tttgaacacc aactctcaca accagcttga gatccgcacc ctgggatccg
     7621 gtgtaatcat ggatcaacgc ggttatatca tcaccaataa acacgtcatc aacgacgccg
     7681 atcagatcat cgtcgcctta caggatggtc gtgtatttga agcattgctg gtgggatctg
     7741 actctctaac cgatctggcg gtacttaaaa ttaatgccac tggcggttta cctaccattc
     7801 cgatcaatgc acgtcgcgta ccgcacattg gcgacgtagt actggcgatc ggtaacccgt
     7861 acaacctcgg gcagaccatt acccagggga ttattagtgc cacgggtcga atcggtctga
     7921 acccgaccgg gcggcaaaac ttcctacaaa ccgatgcttc cattaaccac ggtaactctg
     7981 gcggcgcgct ggtgaactcg ctgggcgaac tgatgggcat taacacgctg tcgtttgata
     8041 agagtaacga tggcgaaacg ccggaaggta tcggctttgc gattcctttc cagttagcaa
     8101 ccaaaattat ggataagctg atccgcgatg gtcgcgtgat ccgcggctac attggtatcg
     8161 gcggacgcga gatcgcacca ctgcacgcgc agggcggtgg tatagatcaa ctgcaaggga
     8221 tcgtggttaa tgaagtgtca cctgacggcc cggcggcgaa tgcgggtatt caggtcaacg
     8281 atctgattat ttcggtggat aacaaaccgg ccatctctgc tctggagacg atggatcagg
     8341 tggcggaaat tcgccctggt tcggtgatcc cggttgtagt gatgcgtgat gataagcagt
     8401 taacgctgca ggtcaccatt caggaatatc cggcaaccaa ttaagtcgag tgctcaaaac
     8461 aaaaaccgga gtctgtgctc cggtttttta ttattcgcta atcaattact tattaacgaa
     8521 ctcttcgccc agggcgatat ctttcttgag cgtatccagc ataccttcca gcgcgttctg
     8581 ttcaaatgcg ctcagggtac cgatagattt acgctcttcc acgccgtttt tacccagcag
     8641 cagcggttga gagaagaaac gggcgtactg accgtcgcct tcaacgtagg cacattcgac
     8701 aacgccttgt tcgccctgca gtgcacgaac cagagacaga ccaaaacgtg cagctgcctg
     8761 gcccatagac agggttgcag acccgccacc ggctttcgct tcaaccacct cagtacccgc
     8821 gttctggata cgtttggtca gatcagccac ttcctgctcg gtaaaactaa cgccaggaac
     8881 ctgtgacagc agcggcagaa tggtaacacc agagtgaccg ccaataaccg gcacttcaac
     8941 tttgcctggc tgtttgcctt tcagttccgc aacaaaagtg ttggaacgaa tgatatccag
     9001 cgtggtaacg ccgaacagtt tgtttttgtc ataaacaccg gcttttttca gcacttcagc
     9061 agcaatcgca actgtggtgt taaccgggtt agtgataata ccaatgcacg ctttcgggca
     9121 ggttttcgca acttgctgta ccaggttttt cacgatgccg gcgttaacgt taaacaggtc
     9181 ggaacgatcc atacccggtt tacgcgctac acctgcagag ataagaacga catctgcgcc
     9241 ttccagcgcc ggagtcgcat cttcaccaga aaaacctttg attttcacag cagtagggat
     9301 atggctcaga tcgacggcca caccgggagt cactggagcg atatcataca gagagagttc
     9361 tgaacctgaa ggcagttggg tttttaacag tagtgcaagc gcctggccaa taccgccagc
     9421 agcgccgagg actgcgactt tcatcctaaa ctccttatta tattgataaa ctaagatatg
     9481 ttgctccgct gccgcgacct taatccacaa aattgccgtt tacaatgacc acatctcaag
     9541 aatgtgtagt cacgcaagtt tagcgtttat gcatttaatt gccgtaatca ggaacctaac
     9601 ttacgtaatt aacagtcgct tatgcattag cgcaacattc caacaggtgg tgacaatata
     9661 ccctaccgtt cagccaaaac aacatcaatt tgataacaat taatttactt ttaagcagaa
     9721 tttgcatgcc gtgacgcagg catgtttctc aataacgaaa tttgataaaa tcccgctctt
     9781 tcataacatt atttcagcct tcttcagggc tgactgtttg cataaaaatt catctgtatg
     9841 cacaataatg ttgtatcaac caccatatcg ggtgacttat gcgaagctcg gctaagcaag
     9901 aagaactagt taaagcattt aaagcattac ttaaagaaga gaaatttagc tcccagggcg
     9961 aaatcgtcgc cgcgttgcag gagcaaggct ttgacaatat taatcagtct aaagtctcgc
    10021 ggatgttgac caagtttggt gctgtacgta cacgcaatgc caaaatggaa atggtttact
    10081 gcctgccagc tgaactgggt gtaccaacca cctccagtcc attgaaaaat ctggtgctgg
    10141 atatcgacta caacgatgca gttgtcgtga ttcataccag ccctggcgcg gcgcagttaa
    10201 ttgctcgcct gctggactca ctgggcaaag cagaaggtat tctgggcacc atcgctggcg
    10261 atgacaccat ctttaccacc cctgctaacg gtttcaccgt caaagacctg tacgaagcga
    10321 ttttagagct gttcgaccag gagctttaat ctctgccccg tcgcttctgg cggcggggaa
    10381 aatgttgctt atccctctca accccctgct ttctcctgcg atacatttaa cgaatagtgc
    10441 gttttactgc gacatgtca
//
LOCUS       DAFNBJ010000166        10447 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000166 DAFNBJ010000000
VERSION     DAFNBJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.166"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            481..708
                     /locus_tag="L2X49_004064"
     CDS             481..708
                     /locus_tag="L2X49_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799070.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(743..883)
                     /gene="ykgO"
                     /locus_tag="L2X49_004065"
     CDS             complement(743..883)
                     /gene="ykgO"
                     /locus_tag="L2X49_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HBN4799071.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(883..1146)
                     /locus_tag="L2X49_004066"
     CDS             complement(883..1146)
                     /locus_tag="L2X49_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBN4799072.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1510..1611)
                     /locus_tag="L2X49_004067"
     CDS             complement(1510..1611)
                     /locus_tag="L2X49_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799073.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            2726..6982
                     /gene="fdeC"
                     /locus_tag="L2X49_004068"
     CDS             2726..6982
                     /gene="fdeC"
                     /locus_tag="L2X49_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HBN4799074.1"
                     /translation="MSHYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNDDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNTISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKMTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQATLEVDGSTPKVANDNDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSDVTLTASSENLVLDPKGTAKTNEQGQA
                     VFTGSTTIAATYTLTAKVEQANGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATMTVTLMAGVNPVGGSMWVDIEAPEGVTEKDYQFLPSKADHFSGGKITRTFSTSK
                     PGVYTFTFNALTYGGYEMTPVKVTINAVAAETENGEEEMP"
     gene            complement(7122..7973)
                     /locus_tag="L2X49_004069"
     CDS             complement(7122..7973)
                     /locus_tag="L2X49_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN4799075.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCEYADMPKEWTTTRGLY
                     ASFRYEGNWENYPDWVRNIYLIELPARGLARVNGSDIERFYYNEDFVEKDGNDVVCEI
                     FIPVRPV"
     gene            8276..8404
                     /locus_tag="L2X49_004070"
                     /pseudo
     CDS             8276..8404
                     /locus_tag="L2X49_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(8564..9157)
                     /gene="rclC"
                     /locus_tag="L2X49_004071"
     CDS             complement(8564..9157)
                     /gene="rclC"
                     /locus_tag="L2X49_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HBN4799076.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(9169..9405)
                     /locus_tag="L2X49_004072"
     CDS             complement(9169..9405)
                     /locus_tag="L2X49_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBN4799077.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKTILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(9514..>10447)
                     /locus_tag="L2X49_004073"
     CDS             complement(9514..>10447)
                     /locus_tag="L2X49_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HBN4799078.1"
                     /translation="VVPPIPGITTTPGVYDSTGLLNLKELPGHLGILGGGYIGVEFAS
                     MFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIILNAHVERISHHENQVQ
                     VHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVVDKRLHTTADNIWAMG
                     DVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFMTPPLSRVGMTEEQAR
                     ESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGASLLCVDSHEMINIVK
                     MVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         2910 a         2265 c         2531 g         2741 t
ORIGIN      
        1 ttatattggt caggaacttt gccctgaagt gttaggaata gtcttaatgc gctgtgtgat
       61 ggactagtaa aaaaacaagt tttaagggta agggatagaa tataaaacta tgatgagctg
      121 tcattgaaga tgatttgcca atcagtaaga tattttaatg gattaacatg ccgcacaaaa
      181 tggttacagg tgttcattcc aataagtaat ggaaaattga acgggcgtat gtacgtcatc
      241 tgttcggtaa gcccccaaga gccagattaa gtgaagttaa acgaaggctg ccagctgtca
      301 caggaacgat ggcctttttg tcagggcact gcattcagtt atctgtgcgc gttactctaa
      361 ctggcactcg aacgtcggcg acggggttac cggtggatca gactgttcct gtggcgtaaa
      421 cgtttttatg ttagtaccaa cagatttacc acatttatca tcttaacagt ttgagtgaaa
      481 atgcaggctg cgccgcaaag ggaggagact gagtggcgtg ttcagtctaa aagagggttg
      541 atgccagcat acaggggcga agctggtcaa caagtcaaca ttaagataat ggaatattcg
      601 gagagaaacg tcaggcagtt ggcctcaaat gaacaggaag aatatatccc caggaaaatt
      661 aatgttggtg ttataaacac accaacatta atccgatctg attattaatc agacaaatat
      721 cgttaatccg tcgaattttg aatcaacgtt ttttcttacg accctgaacg gccttaaaac
      781 gtggattaga tttacaaatc acatatagcc gtccttttcg cttcacaatc tgacagtctg
      841 gatggcgttc ttttgcggta cgcagagagt taagaacttt catcacgccc cctttttcgt
      901 gctaacaaaa cgaccaaaac gttgggtgaa tcgtgcaaca tttccttctg atgccactgt
      961 tctcagcttc cctgtataga acgggtgcga tttagaagag acatcaattg tcacgtatgg
     1021 atacgttacg ccatccagct caatctcacg gtctgttttg atagtcgagc cgattttaaa
     1081 gtactcatca acactggtgt cgtggaacac cacagtacga tactcaggat ggatattggg
     1141 cttcatcatt tttacctgtt atgttataac ataaccataa agatacatgc tcatccggat
     1201 catttcaaca ctcaatccct gtgactcttt gtggatgtgg aattttcgca acggggaact
     1261 ctggcattgt tagcagccat tggtgacgct cccataaacg cctggatgag tgattgatga
     1321 tggttttgct gagagtatgg atttttcgag aatggaggtt atctccactt tactggccga
     1381 tgatttatcg cagtatgatt gattacctga cagcgtgtat tcctctccca cggatataat
     1441 aattacttac aaaaaaggga gaggatgcat attttaaata tcactgaagt gaacagttta
     1501 tttccgttat taatagaaat ggagaaataa ataggcgtat tctacaattg cgacaaaaac
     1561 aacgatatta atcagtttat gactgatttg ctgtacttta ttctctttca ttggtacttc
     1621 ctcgctttaa aaaagagtgc acttcgtaag tgcccttata taaataacga gtttggtcaa
     1681 ccaatttttt gacatgtatc acaaatttga atagatgtat tacatcaact atcttttatt
     1741 gcaccaacgt cattgatata tgtcgcctga agtcagttcc gggaatgagt ctgatctcaa
     1801 gactggccca gtccgggcgt tgattggtgc tgaggagcat atcgcatctc atcataatgt
     1861 cgtatctcct ggggtgttat acaagatatc gttgttggtg acctgggaga ggaattgagt
     1921 tctattaaac cgtcaactat gccggataca tactggatta cactgcaggc acgccttatg
     1981 agagaacgtg ccgcagtgac gggttaatta tctgaaagaa tttgtgaggc tgtatcggtt
     2041 actcattgat ttgatagttt tactctcggg agaataatag atatttaatc cattaacgga
     2101 aaccagccag ttcctttcga tgcctgaatt tgatcccata gtttatccgg aatggttaat
     2161 tcatgaacaa gttgtgttat cgttcatgag aagcataacg taaagggaaa agctcgatta
     2221 gacggcagaa tttgtcaggg gttatgaacg aaattcataa atctgtttga gtgttgcgat
     2281 gggtagtgca agttcgatat ctccgcaatt tacagtccga tgaaggaaaa tgaatatcca
     2341 taaaaaatat attggtttat cctggcatat atacctattt cgacgtattt ccaatagttt
     2401 taattaaagg caggtcattg ttattcactc tgaatagtga attattcact gtccgcagag
     2461 taagaaatat aacttaggta tctatttaat gacttgcaca aaaagctaaa ttttccccca
     2521 taaataaaaa tataatcccg cgcccaacca cctgatgagt ggctacaggc actggatata
     2581 ttaggtggcg gtgcactttc ttacataaag gtatttcctt ttctgcggaa aaggaaatcg
     2641 ggaaatcccc ggtttttctg acaagcagac gccattattt gtgtctgcct atgttcgtta
     2701 attcgttcat caggaaatta tctcaatgtc acattataaa acaggtcata aacaaccacg
     2761 atttcgttat tcagttctgg cccgctgcgt ggcgtgggca aatatctctg ttcaggttct
     2821 ttttccactc gctgtcacct ttaccccagt aatggcggca cgtgcgcagc atgcggttca
     2881 gccacggttg agcatgggaa atactacggt aactgctgat aataacgtgg agaaaaatgt
     2941 cgcgtcgttt gccgcaaatg ccgggacatt tttaagcagt cagccagata gcgatgcgac
     3001 acgtaacttt attaccggaa tggccacagc taaagctaac caggaaatac aggagtggct
     3061 cgggaaatat ggtactgcgc gcgtcaaact gaatgtcgat aaagatttct cgctgaagga
     3121 ttcttcgctg gaaatgcttt atccgattta tgatacgcca acaaatatgt tgttcactca
     3181 gggagcaata catcgtaccg acgatcgtac tcagtcaaat attggttttg gctggcgtca
     3241 tttttcagga aatgactgga tggcgggggt gaacaccttt atcgaccatg atttatcccg
     3301 tagtcatacc cgcattggtg ttggtgcgga atactggcgc gattatctga aactgagcgc
     3361 caatggttat atccgggctt ctggctggaa aaaatcgccg gatgttgagg attatcagga
     3421 acgcccggcg aatggctggg atattcgtgc tgagggctat ttacccgcct ggccgcagct
     3481 tggcgcaagc ctgatgtatg aacagtatta tggcgatgaa gtcgggctgt ttggtaaaga
     3541 taagcgccag aaagacccgc atgctatttc tgccgaggtg acctatacgc cagtgcctct
     3601 tctgacactg agcgccgggc ataagcaggg caagagtggt gagaatgaca ctcgctttgg
     3661 cctggaagtt aattatcgga ttggcgaacc tctggcgaaa caactcgata cagacagcat
     3721 tcgcgagcgt cgggtactgg caggcagccg ctatgacctg gttgagcgta ataacaacat
     3781 cgttcttgag taccgcaaat ctgaagtgat ccgtattgct ctgcctgaac gtattgaagg
     3841 taagggtggt cagacacttt ccctggggct tgtggtcagc aaagcaactc acggactgaa
     3901 aaatgtgcag tgggaagcgc cgtcattact ggctgagggt ggcaaaatta ccggtcaggg
     3961 tagtcagtgg caagtaacgc tcccggctta tcgtccaggc aaagacaatt attatgcgat
     4021 ttctgcggtt gcctacgata acaaaggcaa tgcctcaaaa cgcgtgcaga cagaggtggt
     4081 cattaccgga gcaggtatga gcgccgatcg cacggcgtta acgcttgacg gtcagagccg
     4141 tattcaaatg cttgctaacg gtaatgagca aagaccgctg gtgctgtctc tgcgcgacgc
     4201 cgaggggcag ccagtcacgg gcatgaaaga tcagatcaag actgaactag ccttcaaacc
     4261 ggctggaaat attgtgactc gttccctgaa ggccactaaa tcacaggcaa agccaacact
     4321 gggtgagttc accgaaactg aagcaggggt gtatcagtct gtctttacta ccggaacgca
     4381 gtcaggtgag gcaacgatta ctgttagcgt tgatggcatg agcaaaaccg tcactgcaga
     4441 actgcgggcc acgatgatgg atgtggcaaa ctccaccctg agcgctaacg agccgtcagg
     4501 tgatgtggtt gctgatggtc agcaagccta tacgttgacg ttgactgcgg tggactccga
     4561 gggtaatccg gtgacgggag aagccagccg cttgcgattt gttccgcaag acactaatgg
     4621 tgtaaccgtt ggtgccattt cggaaataaa accaggcgtt tacagcgcca cggtttcttc
     4681 gacccgtgcc ggaaacgttg ttgtgcgtgc tttcagcgag cagtatcagc tgggcacatt
     4741 acaacaaacg ctgaagtttg ttgccggtcc gcttgatgca gcacattcgt ccatcaccct
     4801 gaatcctgat aaaccggtgg ttggcggtac agttacggca atctggacgg caaaagatgc
     4861 ctatgacaac cctgtgacca gcctcacgcc ggaagcgccg tcattagcgg gtgccgctgc
     4921 tgtaggttct acggcatctg gctggacaaa taatgatgat gggacgtgga ctgcgcagat
     4981 tactctcggc tctacggcgg gtgaattaga agttatgccg aagctaaatg gacaggatgc
     5041 ggcagcaaat gcggcaaaag taaccgtggt ggctgatgcg ttatcttcaa accagtcgaa
     5101 agtctctgtc gcagaagatc acgtaaaagc cggcgaaagc acaaccgtga cgcttattgc
     5161 aaaagatgca catggcaaca ctatcagtgg tctttcgttg tcggcaagtt tgacggggac
     5221 cgcctctgaa ggggcgaccg tttccagttg gaccgaaaaa ggtgacggtt cctatgttgc
     5281 tacgttaact acaggcggaa agacgggcga gcttcgtgtc atgccgctct tcaacggcca
     5341 gccagcagcc accgaagccg cgcagttgac ggtcatcgcc ggagagatgt catcagcgaa
     5401 ctctacgctt gttgcggaca ataaggctcc gaccgtcaaa atgacgacgg aactcacctt
     5461 caccgtgaag gatgcgtacg ggaacccggt caccgggctg aagccagatg caccagtgtt
     5521 tagcggtgcc gccagcacgg ggagtgagcg tccttcagca ggaaactgga cagagaaagg
     5581 taatggggtc tacgtggcga ccttaacgct gggatctgcc gcgggtcagt tgtctgtgat
     5641 gccgcgagtg aacggccaaa atgccgttgc tcagccactg gtgctgaacg ttgcaggtga
     5701 cgcatctaag gctgagattc gtgatatgac agtgaaggtt aataaccaac tggctaatgg
     5761 acagtctgct aaccagataa ccctgaccgt cgtggacagc tatggtaacc cgttgcaggg
     5821 gcaagaagtt acgctgactt taccgcaggg tgtgaccagc aagacgggga atacagtaac
     5881 aaccaatgcg gcagggaaag tggacattga gcttatgtca acggttgcgg gggaacacag
     5941 catcacggcc tcagtgaata atgctcagaa gacggttacg gtgaaattca aggcggattt
     6001 cagtaccggt caggcgaccc tggaggttga tggcagcacg ccaaaagtgg caaacgacaa
     6061 tgatgccttt acgctgacgg caacggttaa ggatcaatac ggcaaccttc tgcctggcgc
     6121 tgtggtcgtc tttaatctgc ctcggggcgt caaaccgctt gcagacggta atatcatggt
     6181 gaacgccgac aaggagggta aagcggaact gaaagtggtc tccgtgactg ccggaacgta
     6241 tgagatcacg gcgtcggcag gaaatgacca gccttcgaat gcgcagtctg taacgtttgt
     6301 ggccgataag actacggcga ccatctccag tattgaggtg attggcaacc gtgcagtggc
     6361 ggatggcaaa accaaacaga cgtataaagt tacggtgact gatgccaata acaacctgtt
     6421 gaaggatagc gacgtgacgc tgactgccag ctcggaaaat ttagttctgg atcctaaagg
     6481 gacggcgaaa actaatgagc aaggacaggc tgttttcacc ggctctacca ctatcgcagc
     6541 gacatataca ctcacggcga aagtggaaca ggccaacggt caggtatcga cgaaaactgc
     6601 tgaatctaaa ttcgtcgcgg atgataaaaa cgcggtgctc gccgcatctc cagaacgtgt
     6661 agattctctg gtggcggacg ggaagactac tgcaacaatg acggttaccc tgatggcggg
     6721 agtcaatccc gtaggaggaa gtatgtgggt cgacattgag gctccggaag gagtgacgga
     6781 gaaggattat caattcctgc cgtcgaaggc tgaccatttc tcaggtggga aaatcacgcg
     6841 tacatttagt accagcaagc caggtgtcta tacgttcaca ttcaacgcac tgacgtatgg
     6901 cgggtacgaa atgacgcctg tgaaggtgac aattaacgcc gttgctgcag agactgaaaa
     6961 tggcgaggag gagatgccat aatttgaata gtatgaaagt tacgatctaa taaagaataa
     7021 tggtgttgaa atattcaacg ccattattca ctgaatacct tgcctgatac tgattaaata
     7081 gtatcaggca aggataaaac tcaataagag atagtgacca actaaaccgg acgaacggga
     7141 ataaaaattt cgcaaacaac atcattgcca tccttttcta cgaaatcttc attgtaataa
     7201 aagcgctcaa tatcgctgcc gttcactctg gctaaccccc tggcaggtaa ctctatcaga
     7261 tagatgttac gcacccagtc aggataattt tcccagtttc cttcataacg gaaagaggca
     7321 taaagtcctc gggtcgttgt ccactctttt ggcatgtcag catattcaca taccgtacca
     7381 gcaaaagttt caaccgtgag caaatcgacc gattttgttg acggttcaaa cgtggaaacg
     7441 aaatagatat ccgtcttaaa attcttcata catctggcta aatgatgacg tcgtagtttg
     7501 acttcttcat ccggtgattt attcgacact tcaatgtcat atttaaatga gttgccaaaa
     7561 ataggtgtct caggaaagtt gattaatcta cactccgtct tttgctgttg acgtattaaa
     7621 aaagaaggga agatatttgc gagatcccaa taatcacggt ggcggtattc acggggagag
     7681 cagccgaata actttttaaa ttcgcggctg aatgactgct gtgaatcaaa atgcaaagag
     7741 agtgcaatat caagcataga tttcgcggtg agccggacaa gaatggcggc acgacaaagc
     7801 cttcgtttgc gaatgtattc tcccaaaggc acatgcatga aatttcggaa cagaagctgg
     7861 atgttgcgtc tgctgtagcc agatttctgt gcgatatctt cgattgaaat agggtgctca
     7921 agattgcact caatccactc caggagctgc tgtagaatct tctgcctgat catctcattc
     7981 tccgttaatt ctttgtcata tcgcacattt tcgttatacc ctgggcccta agtgtatcaa
     8041 aaatagcaag ggcgaaggca aaagcactgg tagcgtctgc ttcgttgtcc ccgaaaatag
     8101 agaggtttca gtcctacatt attaatgaat tttttgcata agtgatatca aaatccacat
     8161 actactttga ggttacgttt taacgtagac tcattgttca tgcctaatgg agggactgac
     8221 agtggaattt tccgtatagt ctgcttcaga gtattgcaga tgcccatcaa aaatcgattt
     8281 ctcactgaca gataaagaaa tggcacaaat tagttcgctt gatttgggtt atgttgggga
     8341 gtcggtaaaa cattttaatc ctgaatttgt tcgtggttgt cttgctgtaa aaatacatga
     8401 ttgatattaa tcatgtattt tacctaagac gacaaaaacc ttttaacagg ggagtgatat
     8461 tgatcttcac tctgtcatat ctccggtaat atggcgtcag gcttcatcat cagaaagggg
     8521 gcctgacgcc tgaaaaagtg aacaacagac agtgttcgga ttatcaatat tcagttttta
     8581 acgttgaact ggattcatta ctgcgttgtt taagaatttc ccgcgccgaa tctgccatta
     8641 tcatgactgc acctgccagc atcagagtat ctttcaatac caggcgacca gcaccggata
     8701 aataagggaa accatgatga gcgtcaccca atgcgggtac ccatgcctcc ggggtggtga
     8761 ttaaaaatga gagtgttacc aacggtgtgg taaatgccat cagccctccc aataaaccta
     8821 accagcgatt gacaggatta gccaaaacca gcagagcaat aatcacctcc acgacgccaa
     8881 gaccgttgga aaaaccatag gtattattgg ccgattgcca tgcccttgct tctggtttgt
     8941 attcgccttc gtgagtcaga tactgtttat agtcttccgg gtgttcataa aagaacgaca
     9001 ttagtggact gtttgcgacg aatggtgtaa tgctgtctgc ctcgtaaggg acaaacttta
     9061 ataacccaat ccacataaaa acaattgcaa tactcagacg aatcaatgtc aggccaattt
     9121 tatctccccg acttaacagg tgcaggtatt tttccattgt gaacatcctt atttatacaa
     9181 tactgctgtt gcggttgaat tattatcaat tcgcatcgat gtgactttcc aggatgaagc
     9241 attatatttt ctggctatat cagcaatggc atcctccaga actgtcggtg atgctgatac
     9301 tgacgagacg ctgatatgtt tcagaattgt tttatcatct gcattggtgg aaaatgccat
     9361 aacgccagat aatagtgttg caaataaaac ggatttttta aacatataaa ataccatcaa
     9421 tgaagtgatt aagaagttat agagtaacag ggaattaata agattctttt ctctgagacg
     9481 ccagaatatt tgttctggcg tctgattttg agtttatttg actaatgaaa atagatcatt
     9541 gagtgattcg ctcatcgacg gatgagtaaa tatctgatcg cgtaatatgc tataaggcag
     9601 cccggcatcc atcaccattt tcactatatt aatcatctcg tgggagtcaa cacacagcag
     9661 tgatgcccct aatatacgtt gggttttatt atcaacaatc gcttttaata ccccacgggt
     9721 atcgttcatc actctggcgc gcggaattgc agctacaggc aatgtcacca cctgaatatc
     9781 agcaccactc tctctggctt gttcttctgt cataccaacc ctggacaggg gcggtgtcat
     9841 aaatacggaa taaggcacat ttttccgatc atcagtacta cgtttgcctt cacccagtaa
     9901 ctcatcacgt acaatgcggt aatcatccag tgatatgtaa gtaaattgca gcccgccggt
     9961 aacatctccc atcgcccaaa tattgtctgc ggtggtatgt aatcgcttgt caacgacaat
    10021 tgccccgcgc tcgtttactg cgataccggc attttctgga tgtaacgaag cggtagccgg
    10081 ttgacgaccg gaagctatta acagtgcatc caccgccagt tgggcgtgct cgctatgcac
    10141 ttgcacttga ttttcatggt gactgattcg ctccacatgg gcattgagga taatatcgac
    10201 gccctgatcg cgtaaaatcg tcgcgatatt atcagcaata tcccgttctt cccgaggcaa
    10261 aaacagtgaa gctgcttcta aaatggttac cttgctgcca aaattagcga acatagaggc
    10321 gaactcaacg ccaatatatc cgccgcccaa aatacctaaa tgcccaggca attcttttag
    10381 attaagtaat ccggtgctgt catatactcc tggcgtggtg gtaattccag gaattggcgg
    10441 aaccacg
//
LOCUS       DAFNBJ010000167        10441 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000167 DAFNBJ010000000
VERSION     DAFNBJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.167"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            64..639
                     /gene="ssuE"
                     /locus_tag="L2X49_004074"
     CDS             64..639
                     /gene="ssuE"
                     /locus_tag="L2X49_004074"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBN4799079.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            632..1591
                     /gene="ssuA"
                     /locus_tag="L2X49_004075"
     CDS             632..1591
                     /gene="ssuA"
                     /locus_tag="L2X49_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBN4799080.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLAAFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKT
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1588..2733
                     /gene="ssuD"
                     /locus_tag="L2X49_004076"
     CDS             1588..2733
                     /gene="ssuD"
                     /locus_tag="L2X49_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBN4799081.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2744..3535
                     /gene="ssuC"
                     /locus_tag="L2X49_004077"
     CDS             2744..3535
                     /gene="ssuC"
                     /locus_tag="L2X49_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBN4799082.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3532..4299
                     /gene="ssuB"
                     /locus_tag="L2X49_004078"
     CDS             3532..4299
                     /gene="ssuB"
                     /locus_tag="L2X49_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBN4799083.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4506..7118)
                     /gene="pepN"
                     /locus_tag="L2X49_004079"
     CDS             complement(4506..7118)
                     /gene="pepN"
                     /locus_tag="L2X49_004079"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBN4799084.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7384..8586
                     /gene="pncB"
                     /locus_tag="L2X49_004080"
     CDS             7384..8586
                     /gene="pncB"
                     /locus_tag="L2X49_004080"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBN4799085.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8755..10155
                     /gene="asnS"
                     /locus_tag="L2X49_004081"
     CDS             8755..10155
                     /gene="asnS"
                     /locus_tag="L2X49_004081"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBN4799086.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2359 a         2695 c         2814 g         2573 t
ORIGIN      
        1 acagactgac cgaattgtta tcaatgttaa caaaaaaaga acaattggtt ataaggagag
       61 agtatgcgtg tcatcaccct ggcgggtagt cctcgctttc cttctcgctc cagctccttg
      121 ctggaatatg cgcgggaaaa actaaatggc ctcgatgtag aggtttatca ctggaatctg
      181 caaaacttcg ccccggaaga tctgctttat gctcgtttcg atagtccggc actcaagacc
      241 ttcaccgaac agctgcaaca ggctgatgga ctgatagtcg ctacacctgt gtataaagcc
      301 gcctattccg gcgcgctgaa aaccctgctc gacctgctgc cagagcgcgc tttgcaaggc
      361 aaagtggtgc taccgctggc gacgggcggt accgtggccc atctgctggc ggtcgattat
      421 gcccttaaac cagttttaag cgcactgaaa gctcaggaga tcctgcacgg cgtgtttgcc
      481 gatgactcac aagtaattga ttaccatcac agaccccagt tcacgccaaa tctgcaaacc
      541 cgtcttgata ccgcgctaga aactttctgg caggcattgc accgccgcga tgttcaggtt
      601 cctgaccttc tgtctctgcg aggtaatgcc catgcgtaac atcattaaac tggcgctggt
      661 gggattgctt agcgtctcta cgattgcggt tgctgcagaa tcctcgcctg aagcgttacg
      721 tataggctat cagaaaggca gtattggtat ggtactggca aaaagccacc agttactgga
      781 aaaacgctat ccgcaatcaa aaatctcctg ggtggagttc cccgcgggtc cgcaaatgtt
      841 ggaagcgtta aacgttggca gtattgatct cggcagtacc ggggatattc cgccaatctt
      901 tgcccaggct gccggggctg atttggtata tgtgggcgtc gagccaccga agcccaaagc
      961 cgaagtgatt ctggtggcag aaaacagccc gatcaaaacc gtagccgatc ttaaaggtca
     1021 caaagttgcc tttcagaaag gttccagttc acacaacctt ttactgcgtg cactgcgtca
     1081 ggccggactt aagtttaccg atatccaacc cacttacctg acgcccgctg atgcccgcgc
     1141 cgcgttccag caaggtaacg ttgacgcctg ggctatctgg gatccctact actctgctgc
     1201 attattacag ggcggcgtgc gggtgctgaa agacggcacc gatctcaatc aaactggatc
     1261 gttttatctg gcagctcgtc cctatgcaga aaaaaacggc gcttttattc agggcgtact
     1321 cgcagccttt agtgaggccg atgcgttaac ccgcagccag cgcgagcaaa gcatcgcttt
     1381 actggcaaaa acgatgggct taccggcacc ggtgattgcc tcttacttag atcatcgccc
     1441 tcctaccacc atcaaaacgg ttaacgccga ggttgccgcc ttacagcagc aaacggcaga
     1501 tctgttttat gaaaatcgtc tggtgccgaa aaaagtcgat attcgccagc gcatctggca
     1561 gcccactcaa ctggaaggaa aacaattatg agtctgaata tgttctggtt tttaccgacc
     1621 catggtgacg ggcattatct tggaacggaa gaaggttcac gcccggttga tcacggctat
     1681 ctgcaacaaa ttgcgcaagc ggcggatcgt ctgggctata ccggtgtgct gatcccaacg
     1741 gggcgatcgt gtgaagatgc gtggctggtg gcggcgtcga tgatcccggt gacgcagcgg
     1801 ctgaagtttc ttgtcgccct gcgccccagc gtaacctcac ctaccgttgc cgctcgccag
     1861 gccgccacgc ttgaccgtct ctcaaatggt cgtgcgttgt ttaatctggt cacaggcagc
     1921 gatccacaag agctggcagg cgacggcgtg ttccttgatc atagcgagcg ctacgaagcc
     1981 tcggcggaat ttacccaggt ctggcggcgc ttattgctgg gggaaaccgt caatttcaac
     2041 ggtaaacata ttcatgtgcg cggagcaaaa ctgctcttcc cgccgattca acagccttat
     2101 cctccgcttt actttggcgg atcgtcagac gttgcccagg agctggcggc agaacaagtt
     2161 gatctctacc tcacctgggg cgaaccgcca gaactagtaa aagagaagat cgaacaagtg
     2221 cgggcgaaag ctgccgcgca tggacgcaaa attcgtttcg gtattcgtct gcatgtgatt
     2281 gttcgtgaaa ctaacgacga agcgtggcag gccgccgagc ggttaatctc gcatcttgat
     2341 gatgaaacta tcgccaaagc acaggccgca ttcgcccgga cggattccgt aggacaacag
     2401 cgaatggcgg cgttacataa cggcaagcgc gacaatctgg agatcagccc caatttatgg
     2461 gcgggcgttg gcttagtgcg cggcggtgcc ggaacggcgc tggtgggcga tggtcctacg
     2521 gtcgctgcgc gaatcaacga atacgccgcg cttggcatcg acagctttgt gctttcgggc
     2581 tatccgcatc tggaagaagc gtatcgggtc ggcgagttgc tgttcccaca tctggatgtc
     2641 gccattccgg aaattcccca gccgcagccg ctgaatccgc aaggcgaagc ggtggcgaat
     2701 gattttatcc cccgtaaagt cgcgcaaagc taaggaagaa ataatggcaa cgccagtgaa
     2761 gaagtggtta ttgcgcgttg ccccctggtt tttaccggtg ggcatcgtgg cggtgtggca
     2821 actggcctcc tcagttggct ggctgtcgac gcgtattttg ccctcaccgg aaggggtggt
     2881 gacggcgttc tggacgctct ccgccagcgg cgaactgtgg cagcatctgg cgatcagttc
     2941 ctggcgggcg ctgattggct tttcaattgg cggatcgctg gggttgattt tgggactgat
     3001 tagcgggcta tctcgctggg gagaacggct gctggatacc tcaattcaga tgttgcgcaa
     3061 cgtaccgcat ctggcgctga tcccgctggt gattttgtgg tttggcattg atgaatccgc
     3121 aaaaatcttt ctggtggcgc tcggcacgct attccccatt tatatcaaca cctggcatgg
     3181 catccgtaat atcgatcgcg gtctggtgga aatggcgcgt agctatggat tatccggcat
     3241 accgctgttt atccatgtga tcctgcctgg tgccctgccc tcaattatgg tcggcgtgcg
     3301 ttttgcgttg ggcctgatgt ggctgacgct gattgttgcc gaaaccattt ctgccaattc
     3361 aggcattggt tatctggcga tgaatgcgcg ggagtttttg caaacggacg tggtggtagt
     3421 cgctattatt ctttacgccc tgctcggcaa acttgctgac gtcagcgcgc agctgttaga
     3481 gcgcctctgg ttgcgctgga acccggctta tcatttgaag gaggccacgg tatgaatact
     3541 gctcgtctga accagggcac accattgttg ctcaatgcag taagcaaaca ttacgcggaa
     3601 aatatcgtcc tgaaccaact ggatttacat attccggcag gtcagtttgt ggcggtggtg
     3661 ggccgcagcg gtggtggcaa aagtaccctg ctgcgcctgc tggcaggtct ggaaacgcca
     3721 accgcaggcg atgtgttagc gggcaccaca ccgctggctg agattcagga agacacgcga
     3781 atgatgtttc aggatgcgcg tctgctgccg tggaaatcgg tgattgataa cgttgggtta
     3841 ggccttaaag gccagtggcg cgatgccgcg cgtcgggcgt tggctgcagt aggactggag
     3901 aatcgcgccg gggaatggcc tgccgcactt tctggcgggc agaaacagcg tgtggcgctg
     3961 gcaagagcgt tgattcatcg accgggttta ttgttgcttg atgaaccgct cggggcgctg
     4021 gacgccttaa cgcgactcga gatgcaggat ttgattgtgt cactttggca gcagcacggc
     4081 tttaccgtac tgttggtgac gcatgatgtg agcgaagcgg tagcaatggc tgaccgggtg
     4141 ttgttaattg aagagggaaa aattggtctg gatttgacag tggatatccc ccgtccgcgt
     4201 cgattagggt cggtgcgatt agcggagctg gaagcggaag tattgcaacg ggtaatgcgg
     4261 cgtgggcatt ctgaacaact gattcggcgt catggttaat atgccggatg tggcgcacaa
     4321 aaccagcatt ttcaacggac tagtaggcct gataagacac gtcaagcatc gcatcaggca
     4381 gttgggcacg gctgccggat gcggcgtgaa cgccttatcc agcctacaaa accagcaatt
     4441 tcaacggagt tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat tttggctcaa
     4501 aagtatcaag ccagtgcttt ggtgatcttc tcgtacagat cgccagagag attttccagc
     4561 cctttcaact gttccagcgc cgcgcgcatt ttctcctgac gtttggcatc ataacgtttc
     4621 aggcgaatca gcggttcaat cagacgtgaa gctacctgcg ggttacggct gttgaggtcg
     4681 gtaagcattt ccaccaggaa ctggtaaccg ctgccatctt cggcatggaa cgctgccggg
     4741 ttgctgcccg caaacgcacc aatcaacgaa cgaatacggt tcgggttgct catggtaaat
     4801 gagcgatgct gcaacagacc acgcaccgtc tccagcacat tcgccgccgg gctggtggct
     4861 tgcaggataa accatttatc catcaccaga ccgtcctgat gccacttatc gtcgtactcc
     4921 tgcatcagcg cgtcacggca aggcagctgt gcagcaaccg ccgcagaaag cgctgccagc
     4981 gcatcggtca tattgtttgc ttcatggtat tgcttgctca ccagcacgtc agccagatgc
     5041 gtttcaccaa aagcaaggaa gcgcaggcag gcattacgca gagtgcgttt tgcaatatct
     5101 tcatgctcaa cacggtactc gctctggtaa ttcgcgttgt aaatagccag cagctcatcc
     5161 gccagttcag tcgccagagt acgagtgagt gcttcgcgta cttcggcaat agcaatcggg
     5221 tcgatgatat cgaacagttc agccatttca ttgacagaag gcagcgtcag gatttctgcc
     5281 gccagcgccg gatcaatctt ctcatcgagc agcaccgcgc ggaaagcgtc agccacatgt
     5341 accggcagag acagcggctg cccttgctga tgacgggcga cgttcagctt gatgtaggtt
     5401 gccagcaaac tttgcgccgc atcccaacgg gagaaatcat tacgcgcatg acgcatcagg
     5461 aaggtcagtt gctgatcgct ccacttatat tccagtttca ctggcgcaga gaattcgcac
     5521 agcagcgcag gcaccggctg gaagtagaca ttatcaaaaa caaaggtctg ttccgcctgg
     5581 gtgacgttca gcacggaatt caccggatga ccgcctttct gcaacgggat cactttgcct
     5641 tcgttgtcat acaattcgat ggcaaatgga atatgcagcg gctgtttttc tgcctgatcc
     5701 ggcgtggctg gcgtgcgctg gctgatggtc agggtgtact gctcggtttc cggattgtag
     5761 tcgtctttga cggtcacaat cggcgtgccg gactggctgt accaacggcg gaaatgggag
     5821 agatcgacat tcgacgcatc ttccatcgcc tgcacaaagt cgtcgcaggt tgccgcgctg
     5881 ccatcatggc gctcgaaata gagttgcatc cctttctgga agttttcttc gcccagcagg
     5941 gtgtggatca tgcgaatcac ttccgcgccc ttctcgtaaa cggtcagggt gtagaagttg
     6001 ttcatctcaa tgaccatatc cgggcggatc gggtgcgcca tcgggctggc gtcttccgca
     6061 aactgcaatc cgcgcatggt gcgcacatta ttgatacggt ttaccgcgcg ggaaccaagg
     6121 tcagagctga attcctgatc gcggaagacg gttaaacctt ctttcaggct gagctggaac
     6181 cagtcgcggc aggtgactcg gttaccggtc cagttatgga aatattcatg gccgataacg
     6241 cgttcaatat cgaggtaatc tttgtcagtg gcggtgtcgg tgcgggccag cacatatttg
     6301 gagttaaaga tattcaaacc cttattctcc attgcgccca tattgaagaa atccaccgcg
     6361 acgatcatat agatgtcgag gtcatactcc aggccgaagc gttcttcatc ccatttcatt
     6421 gagtttttca gcgaggtcat cgcccacggc gcgcgatcaa ggttgccgcg atcgacgtac
     6481 agctccagcg ccacttcgcg accagaacgc gtggtaaagg tgtcgcgcag tacatcaaag
     6541 tcgcctgcca ccagcgcaaa caggtagcac ggtttcggga acgggtcctg ccactgtacc
     6601 caatggcgtc cgttttccag ttcaccttgc gcaacgcggt taccgttgga aagcaggaag
     6661 ggatatttgg ttttatcggc aataattttg gtggtaaaac gcgccagcac gtccgggcgg
     6721 tcgagataat acgtaatatg gcggaaacct tcggcttcac actgggtgca aagcgcatcg
     6781 cctgactgat aaagcccttc cagcgccgta ttcgccgccg ggcttatttc attaatgatc
     6841 ttaagcgtaa aacgctccgg caaattactg atgaccagtg cgccctcttc ttctttccag
     6901 gcggtccacg gctcatcatt aatatgaaca gaaaccagtt tgaggtcttc gccgttgaga
     6961 cgaagcggag catctgatgc accatgacgg acagcctggc tgaccgcggt aacgaccgtc
     7021 ttttgcgcgt cgaggtcaaa ggtcaagtca atatcagtaa tctggtaatc cggcgcacga
     7081 taatcgtgac ggtatttggc ttgtggctgt tgagtcataa ataaccttta gcatctttta
     7141 tagagtctgg tgttcagtct attcctgttg cgtaaatcgc gctatgcaga atcttcatct
     7201 tttcaggtac aaacgccttt attgctacat ttttataaca tacaccgcgt aatgccatcg
     7261 accagaaagg tggcatatgg tgtgatcggg gttcaataaa ttgcgaaaca aggtatactc
     7321 cagcagttcc tgaagatgtt tattgtacta aacgctcctg tacgaggacg ctactgcgca
     7381 cctatgacac aattcgcttc tcctgttctg cactcgttgc tggatacaga tgcttataag
     7441 ttgcatatgc agcaagccgt gtttcatcac tattacgatg tgcatgtcgc ggcggagttt
     7501 cgttgccgag gtgacgatct gctgggtatt tatgccgatg ctattcgtga acagattcag
     7561 gcgatgcagc acctgcgcct gcaggatgat gaatatcagt ggctctctgc cctgcctttc
     7621 tttaaggccg actatcttaa ctggttacgc gagttccgct ttaacccgga acaagtcacc
     7681 gtgtccaacg ataatggcaa gctggatatt cgtttaagcg gtccgtggcg tgaagtcatc
     7741 ctctgggaag ttcctttgct ggcggttatc agtgaaatgg tacatcgcta tcgctcaccg
     7801 caggccgacg ttgcgcaagc cctcgacacg ctggaaagca aattagtcga cttctcggcg
     7861 ttaaccgccg gtcttgatat gtcgcgcttc catctgatgg attttggcac ccgtcgccgt
     7921 ttttctcgcg aagtacaaga aaccatcgtt aagcgtctgc aacaggaatc ctggtttgtg
     7981 ggcaccagca actacgatct ggcgcgtcgg ctttccctca cgccgatggg aacacaggca
     8041 cacgaatggt tccaggcaca tcagcaaatc agcccggatc tagccaacag ccagcgagct
     8101 gcacttgctg cctggctgga agagtatccc gaccaacttg gcattgcatt aaccgactgc
     8161 atcactatgg atgctttcct gcgtgatttc ggtgtcgagt tcgctagtcg gtatcagggc
     8221 ctgcgtcatg actctggcga cccggttgaa tggggtgaaa aagccattgc acattatgaa
     8281 aagctgggaa ttgatccaca gagtaaaact ctggttttct ctgacaatct ggatttacgc
     8341 aaagcggttg agctataccg ccacttctct tcccgcgtgc aattaagttt tggtattggg
     8401 actcgcctga cctgcgatat cccccaggta aaacccctga atattgtgat taagttggtt
     8461 gagtgtaacg gtaaaccggt ggcgaaactt tctgacagcc ctggcaaaac tatctgccac
     8521 gataaagcgt ttgttcgggc gctgcgcaaa gcgttcgacc ttccgcatat taaaaaagcc
     8581 agttaatatc atcagggagc taatcggctc ccttttttta cctttaattc cgaaatcttt
     8641 cgctgcattt gcgaattctg cttgtctgat tgcagatgcc aggtaacata ggtatccccc
     8701 catttacggg tggacaagtg tttatttttt ccgactatta acagagagaa tattatgagc
     8761 gttgtgcctg tagccgacgt actccagggc cgcgtagccg ttgacagcga agtcaccgta
     8821 cgcggatggg tacgtacccg ccgagattca aaagctggca tctccttcct cgccgtttat
     8881 gacggttcct gctttgatcc tgtacaggct gtcatcaata attctctgcc caattacaat
     8941 gaagacgtcc tgcgtctgac caccggctgc tcggtcattg tgacgggtaa agtcgtggcg
     9001 tcgccgggcc aggggcaaca atttgaaatt caggccagca aagttgaagt tgctggttgg
     9061 gttgaagatc cagacactta cccgatggcg gcaaaacgcc acagcattga gtatctgcgt
     9121 gaagtcgctc acctgcgtcc gcgcacaaac ctgattggtg ccgtcgcgcg cgttcgccat
     9181 acgctggcgc aggcgctgca tcgcttcttt aacgagcagg gattcttctg ggtttcaacg
     9241 ccactgatta ccgcatctga taccgaaggt gcaggcgaaa tgttccgcgt ttctacgctg
     9301 gatctggaaa acctgccgcg taacgatcag ggcaaagtgg atttcgacaa agacttcttt
     9361 ggtaaagagt ctttcctgac cgtatctggc cagttgaacg gcgaaaccta cgcttgcgca
     9421 ttatccaaaa tttatacctt cggcccgact ttccgtgctg aaaactccaa caccagccgt
     9481 cacctggcgg aattctggat gctggagccg gaagtggcgt ttgctaacct gaacgatatc
     9541 gcgggtctgg ctgaagccat gctgaaatat gtcttcaaag cggttctcga agaacgcgca
     9601 gacgacatga aattcttcgc tgaacgcgta gataaagatg ccgtttcacg tctggaacgc
     9661 ttcattgagg ccgatttcgc gcaggtggat tacaccgacg cagtgaccat tctcgaaaac
     9721 tgcggcagga agtttgaaaa cccggtttac tggggcgtcg atctctcttc tgagcatgag
     9781 cgttatctgg cggaagaaca ctttaaagca ccggtagtgg ttaaaaacta tccgaaagat
     9841 attaaagcgt tctatatgcg cctcaacgaa gacggtaaaa ccgttgcggc tatggacgtt
     9901 ctggctccgg gcatcggtga gatcattggt ggctcccagc gtgaagaacg tctggacgtt
     9961 ctggacgagc gtatgctgga aatgggcctg aataaagaag attactggtg gtatcgcgat
    10021 ctgcgtcgct acggtactgt tccgcattca ggtttcggtc ttggttttga acgcctgatt
    10081 gcttacgtaa ctggcgtgca aaacgtgcgt gatgtgattc cgttcccacg tactccgcgc
    10141 aacgccagct tctgattttc ttcctcaaag agccagcgcc tgctggctct tttttatcct
    10201 ttctgtcaag ttatctgttt gttaagtcaa gcaatctatt tgcaaccccg ccataacgat
    10261 catcctgtta cggaatatta cattgcaaca tttacgcaca aaaaataacc cgcattctta
    10321 ttgcggatta gttttttctt agctgatagc acaattttca tactattttt tggcattctg
    10381 gatgtctgaa agaggatttt gtgccaggtc gataaagttt ccatcagaaa caaaatttcc
    10441 g
//
LOCUS       DAFNBJ010000168        10331 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000168 DAFNBJ010000000
VERSION     DAFNBJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.168"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            572..1099
                     /locus_tag="L2X49_004082"
     CDS             572..1099
                     /locus_tag="L2X49_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBN4799087.1"
                     /translation="MSARGINKVILIGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQLLGRAPQQNAQAQPKPHQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     QQPEPPYGFDDDAPF"
     gene            1258..2505
                     /locus_tag="L2X49_004083"
     CDS             1258..2505
                     /locus_tag="L2X49_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBN4799088.1"
                     /translation="MEKNYRVTTFVMDSGERYCMVVDRASGLPMYYPTLFLTTQIRNR
                     EDAFSTMLAAANNLVMLLRFVESRDIDLEQRFLTKDFFKPHELDDLRDFAQCKQRKGP
                     LTVSSTSWLADAMTDIVDNGTQHSRLTTFANYLHWYAMHILKTTELEVVEKINAMAAQ
                     IKTRRPSKKRRNHDLQDRSLSDVQLDALFEVIQPRSELNPFSIDVQRRNRLMILLLFY
                     LGIRGGELLNIRIQDIDFSANRIRIVRRADEQADSRTNEPNAKTRERLLPLAESLAQE
                     LHSYILQDRRKVRNAKKNDYLFVTYKSGPTLGNPISKGGYHKIFSVVRSISPQLYAAT
                     GHSLRHTWNRKFSERMDAMNEQVSEERQEQLRSYLMGWRDGSGTAATYNKRFIRQKGF
                     EAALALQAGNGTSLPEDFKDDHE"
     gene            2492..4030
                     /locus_tag="L2X49_004084"
     CDS             2492..4030
                     /locus_tag="L2X49_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HBN4799089.1"
                     /translation="MTMNNLLLGLVPERIRARANGYSFLLYDTSWTLDRNITINLADI
                     HHYLSDGTLDGCLRTLAFYASNRSSSHTKNILERFQHMLRETDAREITDTVLINYRSM
                     LSPATEWYLATIRGFLRSWHELGYPGISKEVNRLLDGWTLKGNRKGDAVKRMDPQEGP
                     LTDNELTAFNEGAVRAYEKSLITIAELAIALGTSHTGRRPIQISLLRVVDVLCGKNGK
                     GEPFYVLNVPRAKQRSSSFRINFKPFAISLELWAILSAQARNAIAQVENRLGFELQED
                     DRQQIPLFPDLDVLDAVQSPYEFRQLHATDKLHIPAAEITDTLQYIVEKSDIRSERTG
                     ELLHINARRFRYTTGTRAAREGFGELVIAELLDHADTQNAGVYIKNIPEHVKKLDEAV
                     GFQLAPYAQAFVGALVDSERDAHRGNDPTSRIRTEMGQGVGTCGEHGFCGANVPIPCY
                     TCMHFQPWLDGPHEDVYQGLLNERERVKEITGDIQIAAVLDRSIIALADVIMRCAKRR
                     EELS"
     gene            4047..6080
                     /locus_tag="L2X49_004085"
     CDS             4047..6080
                     /locus_tag="L2X49_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBN4799090.1"
                     /translation="MAEIFLFKPKATLTAAENLEAFISQCRDQLTVFGSDLTWEDPVW
                     PNITVFAKLGIITRKPILEETQDPAFIDFAKAYFRYQQGHSLSRAKNESKALRAVEAA
                     LLQVNGNANIDGLSISVLDEAAELAKHYYSDGSAYHCGREIERLAKFVAENQLVSSAV
                     QNWVNPIKRAEDKNKTSRKAKKNREDKLPSDIALNALAEIFANDPIHERDIFTTSVFA
                     MLMSAPSRITEVLALPVDCEVFETDREGIERYGWRFFAGKGYEGDIKWIPTVMVGIAK
                     TAVARARVLSENARQLAKWIEKHPNKFYRHANCPSVADDEPLTMKQVCMALGFAHYSK
                     KTCRSCLGNRGLAKEDGVHTLNSLWQHTMARLPDDFPWFDKDKGIRYSNALFVLNVNQ
                     FHGNRGCIPVELHKPTNNFFNSDLTPRLSLKGKHASIFDRHGYHAVNGEPVKLTSHQA
                     RHLLNTIIQRGGLSNLEIAKWSGRADVKQNRTYNHMTEYELVGIAERLDPTTALFGPA
                     GEVAKHLPVTMQEFNTLEHAAVHVTEYGYCVHDYTMSPCEKFRDCVNCTEQVCIKGDD
                     AEKLDRIKIRLEKLERLFFLADAAVESGEIGTDRWYQYHKKTVTRLRELVAILENPDI
                     ENGVQIKLRGNDFSQLRRVVAKTSIVAIEQKGKESEEAVMLDDLTTLLGGGLG"
     gene            6073..6492
                     /locus_tag="L2X49_004086"
     CDS             6073..6492
                     /locus_tag="L2X49_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799091.1"
                     /translation="MAKHLDNRDINAIVNLIRGWKEPKLSWSAICEAAEPLVGKLPSR
                     QSLNAHEAIVTAYQTKKEALKGRGPKNPRPASLNIAAARIVNLESEIEELKEENRRYK
                     QQFVIWQYNAYKYGMTEHQLNAQLTKIDRERSDGERR"
     gene            6568..>10331
                     /locus_tag="L2X49_004087"
     CDS             6568..>10331
                     /locus_tag="L2X49_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN4799092.1"
                     /translation="MVKPNWDNFKAKFSENPQGNFEWFCYLLFCQEFKMPAGIFRYKN
                     QSGIETNPITKDNEIIGWQSKFYDTKLSDNKADLIEMIEKSKKAYPGLSKIIFYTNQE
                     WGQGRKSHEPEGDKNADNYLETVGNSNDPKIKIEVDQKAYESGIEIVWRVASFFESPF
                     VIVENEKIAKHFFSLNESIFDLLEEKRKHTENVLYEIQTNIEFKDRSIEIDRRHCIEL
                     LHENLVQKKIVIVSGEGGVGKTAVIKKIYEAEKQYTPFYVFKASEFKKDSINELFGAH
                     GLDDFSNAHQDELRKVIVVDSAEKLLELTNIDPFKEFLTVLIKDKWQVVFTTRNNYLA
                     DLNYAFIDIYNITPENLVIKNLERGELIELSDNNGFSLPQDVRLLELIKNPFYLSEYL
                     RFYTGESIDYVSFKEKLWNKIIVKNKPSREQCFLATAFQRASEGQFFVSPACDTGILD
                     ELVKDGIVGYEAAGYFITHDIYEEWALEKKISVDYIRKANNNEFFEKIGESLPVRRSF
                     RNWISERLLLDDQSIKPFIAEIVCGEGISNFWKDELWVAVLLSDNSSIFFNYFKRYLL
                     SSDQNLLKRLTFLLRLACKDVDYDLLKQLGVSNSDLLSIKYVLTKPKGTGWQSVIQFI
                     YENLDEIGIRNINFILPVIQEWNQRNKVGETTRLSSLIALKYYQWTVDEDVYLSGRDN
                     EKNILHTILHGAAMIKPEMEEVLVKVLKNRWNEHGTPYFDLMTLILTDLDSYPVWASL
                     PEYVLQLADLFWYRPLKETGERYHSMDIEDEFGLFRSHHDYYPESPYQTPIYWLLQSQ
                     FKKTIDFILDFTNKTTICFAHSHFAKNEIEEVDVFIEEGKFIKQYICNRLWCSYRGTQ
                     VSTYLLSSIHMALEKFFLENFKNADSKVLESWLLFLLRNTKSASISAVVTSIVLAFPE
                     KTFNVAKVLFQTKDFFRFDMNRMVLDRTHKSSLISLRDGFGGTDYRNSLHEEDRIKAC
                     DDVHRNTYLENLALHYQIFRSENVTEKDVIERQQVLWGIFDKYYNQLPDEAQETEADK
                     TWRLCLARMDRRKMKITTKEKDEGIEISFNPEIDPKLKQYSEEAIKKNSEHMKYVTLK
                     LWASYKREKDERYKNYGMYEDNPQIALQETKEIIKKLNEEGGEDFRLLNGNIPADVCS
                     VLLLDYFNQLNNEEREYCKDIVLAYSQLPLKEGYNYQVQDGTTSAISALPVIYHNYPM
                     ERETIKTILLLTLFNDHSIGMAGGRYSVFPSMVIHKLWLDYFDDMQSLLFG"
BASE COUNT         3056 a         2088 c         2474 g         2713 t
ORIGIN      
        1 gcccacttcg gctcccggct gcctggcggc atccggtcgt caggcccgac tccgacaggc
       61 agctgctggc gcacccgcca gtctccgccg aacccgctgg cgcggtttcg ggctgccgct
      121 acggtccgat gcgtgaaaac ccggcttgca gcgaccgttc cggtcgcgcc gcccctgtcg
      181 cgccgtgacc tgcgctccct gcacacctga cggtgtgtga ggaaactgcg cctgatggcg
      241 cagcagtctt ccctcctgcc gccacccctg cggcctgttt cccgctgtgg actttccggg
      301 gggcagacgt aaccccctcc cgttccatct gtccagggtg agatgcaccg gccacaaaat
      361 ttttagccat gaacccggct aaaaattttg cgtccgcccc cggacagctg tcccgtgagg
      421 cggtaaacgg gcccccatcg gaaaggtcca ctgcggggag aaacccctgt gaagtggacg
      481 gcaggaggag ctttcgctcc tctgaagggg cacatgaacg tcccgtcgcc ggtatccggt
      541 ttatctcaca tctttcagtc aggaggttct tatgagtgca cgtggtatca acaaggtcat
      601 cctcatcggg cgtctgggca atgatccgga agtccgttac atccccaacg ggggcgcagt
      661 ggcaaacctg caggtggcca catcagaaag ctggcgtgac aaacagacgg gggagatgcg
      721 ggagcagacg gaatggcacc gcgtggtgct gttcggcaag ctcgcggaag tggcaggtga
      781 atatctgcgc aagggcgcgc aggtctacat cgaaggtcag cttcgcaccc gtagctggga
      841 agataacggt atcacccgtt acgtcactga aattcttgtt aagaccacgg gcaccgtgca
      901 gctgctggga cgtgcaccac agcagaacgc tcaggcgcaa ccgaagcctc atcagaatgg
      961 gcagccacag agtgctgacg cgacgaaaaa aggtggcgcg aaaacgaaag gccgtggacg
     1021 taaggccgcg cagccagagc ctcagcctca acaacagcca gaacctcctt atggcttcga
     1081 cgatgatgcg ccattctaga gttaacttgt gagttatttg taaagaatcc atacgcaggc
     1141 ccctgttaat tcaggggttt tttgtttcta tcgtcggaaa aagtgtcttg tataaattgg
     1201 tgatttttca cataaagtgt accttgtaat tgtgtaatgt ttatggggtg tgagtgcatg
     1261 gaaaagaact acagagttac aacatttgtc atggatagtg gcgagcgcta ttgcatggta
     1321 gtggatcgtg cttctgggct tcccatgtat taccccactc tgtttctaac aactcaaatc
     1381 cgcaatagag aggatgcttt ttcaacgatg ttggcggcgg ctaacaacct ggttatgttg
     1441 ttacgctttg tagagagtcg cgacattgac cttgagcagc ggtttttgac caaagacttc
     1501 ttcaagccac atgagctgga tgatctgcga gattttgctc agtgcaaaca aagaaagggg
     1561 ccattaactg tgtcctctac ctcttggttg gccgatgcga tgaccgatat cgttgataac
     1621 gggacacagc attcccggct aacaacgttt gccaactatt tacactggta tgcgatgcat
     1681 atcttaaaga cgacagaact ggaagttgtc gaaaagatca atgctatggc ggcgcagata
     1741 aagacgcgcc gaccatctaa aaagcgcaga aaccacgact tacaagacag gtcattgagt
     1801 gatgttcaat tggacgcgct atttgaagtc atccagccta gatcagagtt aaaccctttc
     1861 tcaatagatg tgcagcgccg caatcggctg atgatcttac ttctgtttta tcttggcatc
     1921 cgaggtggtg aattattaaa tataagaata caggacatag attttagcgc taacaggatc
     1981 cgtatcgtta ggagagcaga tgaacaagct gactctagaa ccaatgagcc taatgctaaa
     2041 actagagaac ggctcttacc gttggctgaa tccttagctc aagaactgca tagttatatc
     2101 cttcaagatc gccggaaagt cagaaatgcg aagaaaaatg actatctgtt tgtaacttac
     2161 aagagtggac caactctggg aaatcctatc tccaaagggg gatatcataa aattttttcc
     2221 gtagtcaggt ctatatcacc gcaattgtat gcagcgacag gacattcgct ccgccacact
     2281 tggaatcgca agttttcaga aagaatggat gccatgaatg agcaagtcag cgaggaacgc
     2341 caagagcaac ttcgttcgta cttaatgggc tggagagatg gctcgggaac cgcagcgact
     2401 tacaacaagc gtttcattcg gcaaaaaggt tttgaggccg ccttggcatt acaagcaggt
     2461 aacggcacta gcttgccaga ggacttcaag gatgaccatg aataacctct tacttgggtt
     2521 agtaccagaa cgaatccgtg ccagggccaa tggctactct tttctgttgt atgacacatc
     2581 gtggacattg gatagaaata tcactattaa tcttgctgat attcaccatt acttgtcaga
     2641 tgggacgcta gatggctgcc tgcgtacact ggcgttttat gcatccaatc gttcttcttc
     2701 tcatacaaaa aatattcttg aacggtttca gcacatgctg cgtgagactg acgcgagaga
     2761 aattactgat acggtattga ttaattatcg gtcgatgctg tcgcctgcaa cagaatggta
     2821 tctagcaacc atccgtggct ttcttcgatc ctggcacgaa ctgggctatc ccggcatcag
     2881 taaggaggtg aaccgcttgc tagatggctg gacgctaaag ggtaatcgta agggggatgc
     2941 ggttaagcgc atggatcctc aagaggggcc actgacggat aatgagctga cagcgtttaa
     3001 cgagggggct gttcgggctt atgaaaagag cttaattact atagctgagt tggctatagc
     3061 acttggcacc agtcatacag gacgtcgtcc cattcagatc tcgctgcttc gtgtggtcga
     3121 tgtcctttgt ggtaaaaatg gaaagggaga gcccttctat gtgttgaatg tgccgcgagc
     3181 caagcagcgc agcagctctt tccgaataaa cttcaaacca tttgcaatat cactggaact
     3241 atgggcaatc ctgagtgctc aggcaagaaa tgcgattgcg caggttgaaa atcgtttggg
     3301 gtttgaattg caggaagatg accggcagca aatccctctt tttcctgatc ttgatgtgtt
     3361 agatgccgtc caatctcctt atgaattccg ccaactgcat gctaccgaca aactccacat
     3421 tcccgccgca gagataaccg acaccctcca atacatcgta gaaaagtccg acatccggtc
     3481 ggaacgtacc ggtgagctat tacatatcaa tgctcgtcgt tttcgttata ccacaggcac
     3541 acgagctgcc cgggaaggct ttggcgagct ggtgattgcg gaactgcttg accacgcgga
     3601 cacccaaaac gcaggcgttt acatcaagaa tatccccgag catgtgaaaa agcttgatga
     3661 ggcggttggc ttccaattag caccatacgc tcaggccttt gtcggggcac ttgtcgattc
     3721 agagagagac gcgcatcgag gtaacgaccc taccagtcgt attcggaccg aaatgggtca
     3781 gggtgttggt acttgtggag aacatggctt ttgtggtgcc aatgttccta ttccttgcta
     3841 cacctgcatg catttccagc cttggctgga cggcccacat gaagatgtct atcaaggact
     3901 gctcaacgaa cgtgaacggg tgaaagagat aactggcgac atccagattg ctgccgtact
     3961 tgatcgcagt attatcgcgc tggcagatgt gatcatgcgt tgtgcaaaac gtcgtgaaga
     4021 gcttagttaa cagggagcaa taaccaatgg ctgagatttt cctgtttaag ccaaaggcta
     4081 cgctgacagc agcagaaaac cttgaagcat tcatatccca gtgccgagat caattgactg
     4141 tattcggctc tgatctcact tgggaagatc ctgtttggcc aaacataact gtatttgcca
     4201 agttgggcat tattacgaga aaacccatac tggaagagac gcaagaccct gctttcattg
     4261 attttgccaa agcctatttc cgttaccagc aggggcatag cctctctagg gcaaagaatg
     4321 aaagtaaagc cctccgtgcg gttgaggcgg ctttgcttca agtaaatgga aacgcaaata
     4381 ttgatggcct gtcgatttct gtgctggatg aagcagctga attggcaaag cattactact
     4441 ccgatggctc cgcatatcat tgtggccgtg agattgagcg gctggctaag tttgtagctg
     4501 aaaaccagct tgtatcaagt gctgtacaaa attgggtcaa tccaattaaa cgtgcagagg
     4561 ataagaacaa aacgagcaga aaggcgaaga aaaataggga agataaactt ccgagtgata
     4621 ttgctcttaa tgcgttggcg gaaatttttg ccaacgaccc aattcatgag cgagacatat
     4681 ttacaacatc ggtttttgcc atgttgatgt cggcaccttc acgtattact gaggtgttag
     4741 cactgcctgt ggattgcgag gtttttgaaa cggatcgtga agggatagaa cgatatggtt
     4801 ggcgtttttt tgcggggaaa ggctatgagg gtgatatcaa atggataccc accgtgatgg
     4861 tcggtatagc taagacagcc gtagctagag ctagagtgct ctctgaaaat gcacgacaac
     4921 tggccaagtg gatcgagaaa cacccgaata agttttaccg ccatgctaac tgtcccagtg
     4981 tagccgatga tgagccattg acgatgaagc aggtttgtat ggcgctcggt tttgcacatt
     5041 actctaaaaa gacgtgcagg tcgtgtcttg gtaatcgagg tttagctaaa gaggatggtg
     5101 tacatactct aaatagcctg tggcagcaca caatggcgcg tttaccggat gactttccgt
     5161 ggtttgataa agacaaggga attaggtaca gcaatgcctt gtttgtactg aacgtaaatc
     5221 agttccacgg gaacagagga tgcataccag ttgagttaca taagccaacc aataatttct
     5281 tcaacagtga tctgacgcct cgtctgtcct tgaaaggaaa acatgccagt atctttgatc
     5341 gccatggtta ccatgctgta aacggagagc cggtgaaatt aacctcacat caggctcgac
     5401 acctgctcaa caccattatc cagcgcggtg gcctgtcaaa cttggaaatt gccaagtggt
     5461 cggggcgggc agatgtgaaa cagaatcgaa cttacaacca catgactgaa tacgaactgg
     5521 ttggcattgc tgaacgcctt gatcccacaa cagcgctatt tgggcctgct ggcgaggtgg
     5581 caaagcatct gcccgtgact atgcaggagt tcaatacctt agagcatgcc gcagtccatg
     5641 tgacggagta tggctactgt gtgcatgatt acaccatgag tccttgtgag aaattccgag
     5701 attgcgtcaa ttgtaccgaa caggtttgca tcaaaggcga tgatgctgaa aagttggatc
     5761 gcattaagat acgtctggag aaattagagc gactgttttt tcttgctgac gcagcagtgg
     5821 aaagtggtga gataggaact gatcgctggt atcagtacca caagaaaact gttacccggc
     5881 ttcgtgaact agtggctatt ttagagaatc cagacattga aaatggtgtc cagattaagc
     5941 tccggggcaa tgatttcagc cagcttcgcc gtgttgtcgc aaagacgtct attgtggcca
     6001 tcgagcaaaa aggcaaagaa agtgaagaag ctgtgatgtt agatgatttg acaactttgt
     6061 taggaggtgg ccttggctaa gcatctggac aatagagaca ttaatgccat cgtaaatcta
     6121 attcgaggat ggaaggaacc aaaactgtcc tggtcagcaa tttgtgaggc ggcagaacca
     6181 ttggtcggaa agttgccgtc aagacagtcc cttaatgcac atgaggcaat agtgactgcc
     6241 tatcaaacaa agaaagaggc gcttaaaggc cgtggcccca aaaatccgcg tccagctagt
     6301 ttgaacattg cagccgcgag gattgtgaat cttgaaagcg aaatcgaaga gcttaaggaa
     6361 gagaaccgga ggtacaaaca acagtttgtg atctggcaat acaatgcata caaatatgga
     6421 atgacagaac atcaacttaa tgcgcagctg acaaagattg accgtgagcg ctctgatgga
     6481 gaaagaagat agttgctgag tacgatatcg agggtacatt tctctgtgta ggggtagtta
     6541 tttacaaaaa aataggagaa taattaaatg gtcaaaccaa actgggataa ctttaaagct
     6601 aaatttagtg agaatcctca aggtaatttt gagtggtttt gctacttgtt gttctgtcaa
     6661 gaattcaaaa tgcccgcagg tatatttaga tataagaatc aatctggtat cgaaactaat
     6721 ccaataacca aagataatga aattatcggt tggcaatcta aattctatga cacaaaattg
     6781 tcggataaca aagctgatct tatagaaatg attgagaaaa gcaaaaaggc ttatccagga
     6841 ttaagtaaaa tcattttcta tactaatcaa gagtgggggc aggggagaaa gtcccatgaa
     6901 cctgaaggcg ataagaacgc tgataattat ttggaaactg tcggaaatag taacgatccc
     6961 aaaataaaaa ttgaagttga tcagaaagca tatgagtcgg gtatcgaaat agtatggaga
     7021 gttgctagtt tttttgaatc accgtttgta atagttgaga atgaaaagat tgctaaacat
     7081 ttcttctccc ttaatgaaag catctttgat ttattagaag aaaagcgcaa gcacacagaa
     7141 aatgttttat atgaaattca aaccaatata gagttcaaag acagaagtat tgaaattgac
     7201 agacgacatt gtatagaact tctacatgag aatctagttc agaaaaaaat tgtcatcgtc
     7261 agcggagaag gtggggttgg aaaaacagca gttatcaaaa aaatttatga agcagaaaaa
     7321 caatacactc ctttctatgt ctttaaggct agcgagttta aaaaggacag tattaatgag
     7381 ctattcggtg cgcatggctt agacgatttc tctaatgctc atcaagacga attacgtaaa
     7441 gtcatagtcg tagattctgc tgaaaagctt ttagaactga ccaatatcga tccttttaaa
     7501 gaattcctga ctgttttaat aaaggataaa tggcaggttg ttttcacaac ccgtaacaac
     7561 tacttggcag atctgaacta tgctttcata gatatttata atataactcc tgaaaactta
     7621 gtaataaaga accttgaacg cggcgagcta atagagttat ctgataacaa tggatttagc
     7681 cttcctcaag atgttcgatt attagaacta atcaaaaatc cattttatct aagtgaatat
     7741 ttgaggttct ataccggtga aagcatcgat tatgtgagct tcaaagaaaa gctttggaat
     7801 aagattattg tcaaaaataa accttctcgg gagcagtgtt tcttagcgac tgcttttcag
     7861 cgggctagtg agggccaatt ttttgtctcc ccggcatgtg atactggaat tttagatgag
     7921 ttagttaaag acggaattgt cggctatgaa gctgctggtt acttcattac acatgatata
     7981 tacgaggaat gggcattaga aaagaaaatt tctgtcgatt atatccgtaa agcgaacaat
     8041 aacgagttct tcgaaaaaat aggagaatca cttcctgttc gccgtagttt tcggaattgg
     8101 atatctgaac gattgctttt agatgaccag tccataaagc cttttatcgc agaaatagtc
     8161 tgtggagaag gaatatcaaa tttttggaaa gacgagttat gggtagctgt ccttctttcc
     8221 gataattcaa gcatattttt taattacttt aaaagatatt tacttagtag tgaccagaat
     8281 ctattaaaaa gacttacttt cttattgagg cttgcttgca aggacgttga ttacgatctg
     8341 cttaaacagt taggtgtaag taattcagat ctgctttcca ttaaatatgt tcttactaag
     8401 cctaagggaa ctggttggca gagtgtgatc caatttatct atgaaaattt agatgaaata
     8461 gggatcagaa atattaattt tatacttcct gtgattcagg agtggaatca aagaaacaaa
     8521 gtgggtgaaa cgactcgatt atctagtttg atagccctaa aatattatca atggactgta
     8581 gatgaggatg tctatttatc cggaagggat aatgagaaaa atattctgca tacgattctt
     8641 catggggcgg ccatgattaa acctgaaatg gaagaggttt tagttaaggt tcttaaaaat
     8701 aggtggaacg agcacggtac cccatatttc gaccttatga ccttaatcct tactgactta
     8761 gattcatatc cggtttgggc atctctcccg gaatatgttc tacaattggc agatctgttc
     8821 tggtatcggc cacttaaaga aacaggcgaa cgttatcaca gtatggatat tgaagatgag
     8881 ttcggtctat ttaggtctca tcacgactat tatccagaaa gtccatatca gactcctata
     8941 tattggttac tacaatcaca gttcaaaaaa acaatagact ttattcttga ttttacgaac
     9001 aagacaacga tatgttttgc ccactcccat tttgctaaaa acgaaattga agaagtcgat
     9061 gtctttattg aagaaggaaa gtttataaag caatatatat gcaatcgtct gtggtgctca
     9121 tatcgaggaa cacaggtctc tacctactta ctttcatcaa ttcatatggc attggaaaaa
     9181 ttttttcttg agaattttaa aaatgcagac tcgaaagtgt tggaaagttg gcttcttttc
     9241 ttgttaagaa ataccaagtc agcttctatt tctgcagtag ttacgagtat tgtacttgca
     9301 ttccctgaga agacattcaa tgtagctaaa gtactattcc aaacaaagga tttcttccgt
     9361 tttgatatga atcgaatggt tctagacaga acacataaaa gttcattaat ctccctcagg
     9421 gatggctttg gcggtacaga ttacagaaac tctttgcacg aagaagatag aattaaagct
     9481 tgcgatgatg tgcatagaaa tacttatctt gaaaatcttg ccttgcatta tcaaattttc
     9541 aggagtgaaa atgtaacgga gaaagatgtc attgaaaggc aacaagtgct ctggggtatt
     9601 ttcgacaaat actataatca gcttccagat gaagctcaag aaactgaagc cgataagacg
     9661 tggaggctct gcttggcaag aatggatcgg cgaaagatga aaataactac caaggagaaa
     9721 gatgaaggga ttgagatatc attcaatcct gagattgacc ctaaactaaa gcaatatagt
     9781 gaggaagcaa taaagaaaaa ctccgagcat atgaagtatg taacgctgaa actatgggca
     9841 agctataaaa gagaaaagga tgaacgttat aagaattatg gaatgtatga ggacaatccg
     9901 caaattgctt tacaagagac caaagaaata ataaaaaagc ttaatgagga agggggtgaa
     9961 gatttcagac tattaaatgg taatatacca gcagacgttt gttctgtatt actgttagat
    10021 tactttaatc agttgaataa tgaagagaga gaatattgta aagatattgt tctagcatat
    10081 tctcaacttc cgttgaagga aggctataat tatcaggtac aagatgggac aacctcggca
    10141 atttcagcct tacccgtgat ttatcataat tatccaatgg aaagggagac tataaaaaca
    10201 atattacttt tgacactgtt taatgaccac tctattggaa tggcaggtgg gcgctactca
    10261 gtatttccta gtatggtgat tcataaatta tggctagact attttgatga tatgcagtct
    10321 ctattgtttg g
//
LOCUS       DAFNBJ010000169        10113 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000169 DAFNBJ010000000
VERSION     DAFNBJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.169"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            118..333
                     /gene="bdm"
                     /locus_tag="L2X49_004088"
     CDS             118..333
                     /gene="bdm"
                     /locus_tag="L2X49_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HBN4799093.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            435..572
                     /gene="sra"
                     /locus_tag="L2X49_004089"
     CDS             435..572
                     /gene="sra"
                     /locus_tag="L2X49_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HBN4799094.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            729..2426
                     /gene="maeA"
                     /locus_tag="L2X49_004090"
     CDS             729..2426
                     /gene="maeA"
                     /locus_tag="L2X49_004090"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBN4799095.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2560..3570
                     /gene="adhP"
                     /locus_tag="L2X49_004091"
     CDS             2560..3570
                     /gene="adhP"
                     /locus_tag="L2X49_004091"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBN4799096.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVCTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3716..4000
                     /locus_tag="L2X49_004092"
     CDS             3716..4000
                     /locus_tag="L2X49_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBN4799097.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4407..5060)
                     /gene="fdnI"
                     /locus_tag="L2X49_004093"
     CDS             complement(4407..5060)
                     /gene="fdnI"
                     /locus_tag="L2X49_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBN4799098.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(5053..5937)
                     /gene="fdnH"
                     /locus_tag="L2X49_004094"
     CDS             complement(5053..5937)
                     /gene="fdnH"
                     /locus_tag="L2X49_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBN4799099.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5950..8997)
                     /gene="fdnG"
                     /locus_tag="L2X49_004095"
     CDS             complement(5950..8997)
                     /gene="fdnG"
                     /locus_tag="L2X49_004095"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8410..8412),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBN4799100.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQSEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9229..10110
                     /gene="yddG"
                     /locus_tag="L2X49_004096"
     CDS             9229..10110
                     /gene="yddG"
                     /locus_tag="L2X49_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBN4799101.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2455 a         2640 c         2564 g         2454 t
ORIGIN      
        1 atcatcaacc aggactaatc ttaacaacga agcggcaaat atttgccatg ctgaatgtgc
       61 tttataaatc tgcgatccgt agcagacacc ataaatacac agacacggag aatcactatg
      121 tttacttatt atcaggcaga aaattcaaca gcagaacccg ctctggttaa tgccattgag
      181 caaggccttc gggcacagca cggtgttgtc actgaagatg acattctcat ggagctgacc
      241 aaatgggttg aagcttctga taacgacatc ctcagtgata tctaccagca aacgatcaat
      301 tatgtggtca gtggccagca ccctacgctt taaggtgcta tgcttgatcg gcaacctaat
      361 ttaggggttt agcacgtgtt tcttcgctac ggcgatgttg tccttaaaac tagcaacagg
      421 attgaggagt taaaatgaaa tcgaaccgtc aggcacgtca tattcttgga ctggaccata
      481 aaatttctaa ccagcgcaaa atagttaccg aaggtgacaa atccagcgtg gtaaataacc
      541 caaccggcag aaaacgcccc gctgaaaagt aattcataac catcagtcct caatgacgat
      601 taaacaccat tgcctgcgca atggtgtttt tgtttttatc tgctttatac ttggggccga
      661 cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt caaaaaagag
      721 tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct tacgctggcc
      781 ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg gaagaacgcc
      841 gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa gaacaagcgg
      901 aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac atctacctgc
      961 gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat catcttgatg
     1021 agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt ttttctgaga
     1081 tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccggcacaat atggacgata
     1141 ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac ggtgaacgta
     1201 ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt aaactgtcgc
     1261 tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg ctggatgtcg
     1321 gaacgaacaa ccaacagctg cttaacgatc cgctgtatat gggctggcgt aatccgcgta
     1381 tcactgacga tgaatactat gaattcgttg atgaatttat ccaggctgtg aaacaacgct
     1441 ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg ccgttactta
     1501 accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcact gcggcagtaa
     1561 cagtcggcac actgatcgca gccagccgcg cggcaggtgg tcagttaagc gagaaaaaaa
     1621 tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc atcgcccaga
     1681 cccagcgcga aggattaagc gaggaagcag cgcggcagaa agtctttatg gtcgatcgct
     1741 ttggcctgct gacggacaag atgccgaacc tgctaccttt ccagaccaaa ctggtgcaga
     1801 agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gttgtcactg ctggatgtgg
     1861 tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg ctgtttacgg
     1921 aagagatcat tcgtgagatg cataaacact gtccgcgtcc gatcgtgatg ccgctgtcta
     1981 acccgacgtc acgcgtggaa gccacaccgc aggacattat cgcctggacc gaaggtaacg
     2041 cgctggtcgc cactggcagc ccgtttaatc cagtggtatg gaaagataaa atctacccta
     2101 tcgcccagtg taacaacgcc tttattttcc cgggcatcgg cctgggggtt attgcttccg
     2161 gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag tgaaacgctt gctcagtatt
     2221 cgccgctggt cctgaacggc gaaggtctgg tactaccgga actgaaagat attcagaaag
     2281 tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc gtggcggtga
     2341 aaacatctgc cgaagctctg caacaggcca ttgatgataa tttctggcaa gccgaatacc
     2401 gcgactaccg ccgtacctcc atctaagcct gcgcccggta gtgaaggcta ccgggctatt
     2461 tctctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat ctcatttgcc
     2521 tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaaggctgc agttgttacg
     2581 aaggatcatc acgttgacgt tacggataaa acactgcgct cactgaaaca tggcgaagcc
     2641 ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa gaatggcgat
     2701 tttggtgaca aaactggcgt aattctgggc catgaaggca tcggtgtggt ggcagaagtt
     2761 ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg gttctacgaa
     2821 ggatgcggtc attgtgaata ctgtaatagt ggtaacgaaa cgctctgccg ttcagttaaa
     2881 aatgccggat acagcgttga tggcgggatg gcggaagagt gcatcgtggt cgccgattac
     2941 gcggtaaaag tgccagatgg tctggactcg gcggcggcca gcagcattac ctgtgcgggg
     3001 gtcaccacct acaaagccgt taaactgtca aaaattcgtc cagggcagtg gattgctatc
     3061 tacggtcttg gcggtctggg taacctcgct ctgcaatacg cgaagaatgt ctttaacgcg
     3121 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatgggtgca
     3181 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
     3241 ggcgctcacg ctgcggtggt aacagcagtt gctaaagctg cgtttaactc ggcagttgat
     3301 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
     3361 gatatcccac ggctggtgct ggatggcatt gaagtggtcg gttcgctggt ctgcacgcgc
     3421 caggatttaa ctgaagcctt ccagtttgcc gccgaaggta aagtggtgcc gaaagtcgcc
     3481 ctgcgtccgt tagcggacat caacaccatc tttactgaga tggaagaagg caaaatccgt
     3541 ggccgcatgg tgattgattt ccgtcactaa gaggcctttg ctgcgactgc catgttcggg
     3601 tcgcagcatc gcacactctc caacatgaaa tggctaaatg gattattgtt gcgtaggaag
     3661 gaacaataat gatagtgttc ccagtgaagg aacgctactg agagtaaaga cactaatgaa
     3721 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
     3781 cagcctgcgc gagtttgcca gagcaatgga aattgctccc tcaacggcaa gccgattgct
     3841 gacaggaaaa gcagctttga cgccagaaat ggcgattaaa ctttccgtgg tgatcggcag
     3901 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac
     3961 agtggatgtg tcgcgattac gccgtctggt tacgcaataa aaaaatggca ctttataaag
     4021 cgccatttag aactgatgac aaacgcaaca ttgcctgatg cgctacgctt atcaggccta
     4081 cacgttcact gcaatctatt ggatttgaag gggttgtagg ccggataagg cgttcacgcc
     4141 gcatctggca tgaacaaagc ctacgttgtc tacaatatga aaatgacgcc ttttacagcg
     4201 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     4261 cgttcactgc aatctattgg atttgaaggg tttgtaggcc ggataaggcg ttcacgccgc
     4321 atccgggatg aacaaagcct acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     4381 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     4441 gccttctcga tttcacgata ccagcgtgga tggtgtttct tcgcccagcg acggctcacc
     4501 ttcccttcga tcatcccttt aatcgaccct ttcacccaaa atgccatata catatggatc
     4561 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     4621 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     4681 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     4741 ccgacatccg ccactttatg ctcattgcct ttcaatactt cgacaatgtt caacagccac
     4801 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4861 gcgacgaaaa tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg
     4921 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4981 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     5041 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     5101 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     5161 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     5221 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     5281 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     5341 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     5401 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     5461 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     5521 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     5581 aatgattgca ccagcagacg ggcacgcctt caggcagccg ggatcttcac agtgcataca
     5641 gccgtcttta cggatcagcc actccagctt gccgttctgt tcggtttcgc taaagcgcat
     5701 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     5761 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5821 acaggtggaa acgtcgataa gttttgcgac ttctgctttg taatcacgca cctgagaagg
     5881 cggcgtgatg gagttagttg cggacctttt gataatgtcc tgcgtttcca tagccatctg
     5941 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     6001 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     6061 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccat ttacattcag
     6121 cggtttcaga cgacgcgtta ccacagccac cgcgcggata aagccacgct tgctggagac
     6181 agtgacacga tcgccattat taatgccttt cgccgccgcc agcgtttcgc tgatttccac
     6241 aaactgttcc ggctgagcaa ttgcgttgag caatgcgtgc ttggtccagg tgtggaagtg
     6301 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     6361 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     6421 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     6481 gttgatggca aacaggcgtc ccatcccttc cggctgcatg ataaacggcc cggttggcgt
     6541 acccggtgcg gcattgccga agtcaggaat atcgttaccc gtccacttgc tgccgttcca
     6601 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     6661 gtacagcacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     6721 ggacgggtcg gagttatcgc ggttagccat ctggttgccc tgctctgtcc agctaccggt
     6781 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6841 caactgacct ttcttcgcaa taagcacgcc attagcgtca tagagatctt ccagcgcgta
     6901 gccgttgttc tctttagcca cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6961 gctcatcttc atcagcggtt ctacgccttt accaccttcg gactggtaca gctcgcgcag
     7021 atgatggtag atacccgcca gaatttcacc gtcgttacgc gcttcgcccg gcgcgtcctg
     7081 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cttcttcagc
     7141 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     7201 cgactcaccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     7261 catgtacttc agcttgctca ggcagctcac cactttgttt ttgtccggga aggacgcaac
     7321 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     7381 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     7441 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     7501 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     7561 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     7621 caggcctaag tcagtcaagc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     7681 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     7741 cgcacccaca gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7801 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccgca
     7861 gatgttttca acgacgtccg gcgtgtagcg ggaaacgtgc tctttcagca ggttccacac
     7921 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7981 gttccaggac gatttatcgt attgacgttt ttcagcgtcg tagccgctga acagaccgtc
     8041 ttcgaaagca aaatcatcac gcaccagcag gctggcgttg gtgtaatgct taacgtattc
     8101 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     8161 cgtaccggaa cgaataggcg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     8221 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     8281 gacgggatgc gcttcagcag cgttaccgcc catcaccatc acgacgttag cgtttttgat
     8341 atccacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     8401 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     8461 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     8521 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     8581 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     8641 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcactgt tgacgtaatc
     8701 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     8761 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8821 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8881 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctactgt
     8941 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     9001 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     9061 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     9121 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     9181 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     9241 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     9301 cgcggtgtca gtgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     9361 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcaaatccc gaaaggctat
     9421 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     9481 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     9541 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggtt gattgtacct
     9601 ggattattat tagccctcgt cggcgtctgc tgggtgttag gcggtgacaa tgggttacat
     9661 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgttcatt
     9721 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9781 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9841 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9901 accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9961 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
    10021 agcgccccgc tgtcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg
    10081 ctctgctggc tggcgacacg tcgtggttaa aat
//
LOCUS       DAFNBJ010000170        10026 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000170 DAFNBJ010000000
VERSION     DAFNBJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.170"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            157..660
                     /locus_tag="L2X49_004097"
     CDS             157..660
                     /locus_tag="L2X49_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBN4799102.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(739..990)
                     /locus_tag="L2X49_004098"
     CDS             complement(739..990)
                     /locus_tag="L2X49_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBN4799103.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1105..1191)
                     /gene="azuC"
                     /locus_tag="L2X49_004099"
     CDS             complement(1105..1191)
                     /gene="azuC"
                     /locus_tag="L2X49_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBN4799104.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1454..1777
                     /locus_tag="L2X49_004100"
     CDS             1454..1777
                     /locus_tag="L2X49_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBN4799105.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1948..2445
                     /gene="ftnA"
                     /locus_tag="L2X49_004101"
     CDS             1948..2445
                     /gene="ftnA"
                     /locus_tag="L2X49_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBN4799106.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2483..2722)
                     /locus_tag="L2X49_004102"
     CDS             complement(2483..2722)
                     /locus_tag="L2X49_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBN4799107.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            2913..4124
                     /gene="tyrP"
                     /locus_tag="L2X49_004103"
     CDS             2913..4124
                     /gene="tyrP"
                     /locus_tag="L2X49_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBN4799108.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4186..4851)
                     /gene="yecA"
                     /locus_tag="L2X49_004104"
     CDS             complement(4186..4851)
                     /gene="yecA"
                     /locus_tag="L2X49_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBN4799109.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5047..5133)
                     /locus_tag="L2X49_004105"
     tRNA            complement(5047..5133)
                     /locus_tag="L2X49_004105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5097..5099),aa:Leu,seq:taa)
     gene            complement(5146..5219)
                     /locus_tag="L2X49_004106"
     tRNA            complement(5146..5219)
                     /locus_tag="L2X49_004106"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5185..5187),aa:Cys,seq:gca)
     gene            complement(5274..5349)
                     /locus_tag="L2X49_004107"
     tRNA            complement(5274..5349)
                     /locus_tag="L2X49_004107"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5314..5316),aa:Gly,seq:gcc)
     gene            complement(5501..6049)
                     /gene="pgsA"
                     /locus_tag="L2X49_004108"
     CDS             complement(5501..6049)
                     /gene="pgsA"
                     /locus_tag="L2X49_004108"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBN4799110.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6106..7938)
                     /gene="uvrC"
                     /locus_tag="L2X49_004109"
     CDS             complement(6106..7938)
                     /gene="uvrC"
                     /locus_tag="L2X49_004109"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBN4799111.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7935..8591)
                     /gene="uvrY"
                     /locus_tag="L2X49_004110"
     CDS             complement(7935..8591)
                     /gene="uvrY"
                     /locus_tag="L2X49_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBN4799112.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8887..9063
                     /locus_tag="L2X49_004111"
     CDS             8887..9063
                     /locus_tag="L2X49_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799113.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9050..9274
                     /locus_tag="L2X49_004112"
     CDS             9050..9274
                     /locus_tag="L2X49_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBN4799114.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9342..10025)
                     /gene="sdiA"
                     /locus_tag="L2X49_004113"
     CDS             complement(9342..10025)
                     /gene="sdiA"
                     /locus_tag="L2X49_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HBN4799115.1"
                     /translation="MLRFQRMEAAEEVYHEIELQAQQLEYDYYSLCVRHPVPFTRPKV
                     AFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSEAQPLWEAARAHGL
                     RRGVTQYLMLPNRALGFLSFSRSSAREIPILSDELQLKMQLLVRESLMALMRLNDEIV
                     MTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNMQKKINAPNKTQVA
                     CYAAATGLI"
BASE COUNT         2513 a         2523 c         2313 g         2677 t
ORIGIN      
        1 atacccgttc agacgttatt cttatttcag atcatcgtca gaattgactc cacgatcaca
       61 tttcggaccg gcagaaagga attattctgc aaacagtaat tatggtgttt tgatttatct
      121 tgcacctctc cacttctgga tataaggata ttaggtatgg caaccgctgg aatgcttctc
      181 aaactcaact ctcaaatgaa ccgcgagttt tacgcatcca atctctacct tcacctgagt
      241 aactggtgtt ctgaacagag tctgaacggc accgccactt tccttcgcgc ccaggcacag
      301 agtaatgtga cccaaatgat gcgcatgttt aactttatga agagtgtcgg cgctaccccc
      361 atcgttaaag ccattgatgt tcccggtgaa aaactgaact ctctggaaga actgttccaa
      421 aaaacgatgg aagaatacga gcaacgttct agtacgttgg cacagttagc cgatgaagcg
      481 aaagaactga atgatgattc aaccgtcaat ttcctgcgcg atctggaaaa agaacagcag
      541 catgatggtc tgttgctgca aaccattctt gatgaagtgc gcagtgcgaa acttgcgggt
      601 atgtgccctg tgcagaccga ccaacatgtt ctgaatgtcg tgtcacacca gctgcattga
      661 tcatcatcgg cgctaatgca ttgcgccgat gaaggttttg agaaaccgct gcctcatctg
      721 tttgaagcag cggttttttt aatgggattc accctgtggg gtaaacttga gttcaataag
      781 cgcgatggct ttttggattg cccgcatggt gaccgggtct gcggcggcgg gatggttagt
      841 aaagtcgata ttcttcagct gactggacat tttttcacga acttcaacgg gcgcgattac
      901 atcgagaaca tccagaattt gtttgataac caactggcaa gcaaccacat cagaaaccag
      961 ttcctgatcg gcattcagcg gctgggacat cgtaaactcc tgatagcatt ttgaaagccg
     1021 ttatagtagc ggcttcacat cttcagcgat agtcacatcc accgtcatca ggacacaaaa
     1081 aaacctgccg gagcaggttt tttgttatcg gaacatattg cctggcggta cgtctttgaa
     1141 cgtcttgcaa tagttattga acatactttt caggattttg cgcagtttca tcgcggcact
     1201 ccgaccattt gttatacagg tgttattgtc tttgcgctta taatatgaca accatcacaa
     1261 aaatcaatct ttatgtgata caaatcacat aaatacccct ttaatgttat aaaaatgata
     1321 atcaaaaaac agccccccta tttctgacac ctacagatgg caagaaatag cgcctgccag
     1381 gcgtcttttc cggccattgt cgcagcgctg taacgcgtaa aatagtgctt tctcttactc
     1441 ttctggctgg accatgagac ttctgattct gactctttca ttaataacgc tggcggggtg
     1501 tacggttact cgccaagccc acgtgagcga agttgacgcg gcaacaggta ttgtacggtt
     1561 ggtttatgac caggcttttt tgcagcatgc ccataccgat cgttatgtca gtcgcgggat
     1621 cgccgatcgc gcatgccagc aggaaggcta tacccacgcg atcccctttg gtcagccagt
     1681 aggcaactgt agcctttttg cgggttcctt atgtttgaat acggaattca ctttgtcata
     1741 tcagtgccat cattccgcct tccctgtctt tctctaaata ttttaatcag cgaggggatc
     1801 ttcgctgatt aaataaatag cggtaatgcg ttttattccc attcgtattt ttaataattg
     1861 aagtttatat tttacctttt gcaaataata aaataacaaa ttatagtgac gccacagcaa
     1921 caaatataac ctttgtggag cactatcatg ctgaaaccag aaatgattga aaaacttaat
     1981 gagcagatga acctggaact gtactcttca ctgctttatc agcaaatgag cgcctggtgc
     2041 agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca ggaagagatg
     2101 acgcatatgc agcgtctgtt tgattacctg actgataccg gcaatttacc gcgtattaat
     2161 accgttgaat ctccgtttgc tgaatattcc tcacttgatg aattattcca ggaaacctat
     2221 aaacacgaac aattaatcac ccagaaaatt aacgaactgg ctcatgctgc aatgaccaat
     2281 caggactacc caacatttaa tttcctgcaa tggtatgttt ctgagcagca tgaagaagag
     2341 aaactgttca aatcgattat tgataaatta agcctggcag gcaaaagcgg cgaaggtctg
     2401 tattttatcg acaaagaact ctctaccctc gacacacaaa actaatgctc tccggcggca
     2461 ggtttactgc cgccgtttca aatcagtgac ggcaaatctt actctgatcg gtcgaaaaac
     2521 cgtctttcga aggtataaat ttgccttttg ctgccagaaa cgctaccagc tctcccgctg
     2581 tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc accaaaacgc gctttaatcg
     2641 cagcttccag actggcatgc gtatattgct cgcctgattc aatcatcata tttaacactt
     2701 catgaccgtg aatagagtcc atcgtccctc ctcaaaaaaa gcctagcgta gcgattgccg
     2761 cttatgaaga ctttgcgcca gcgcaggact gaatgctttt tattgtacat ttatatttac
     2821 accatatgta acgtcggttt gacgaagcag ccgttatgcc ttaacctgcg ccgcagatat
     2881 cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa cagaaccctg ggaagtgttt
     2941 ttatcgtggc gggaaccaca attggcgcag gcatgctggc aatgccgctg gctgcggccg
     3001 gtgttggttt tagcgttacg ttaatcttgt tgattgggct ttgggcgttg atgtgctaca
     3061 cggcgctatt actgctggag gtgtaccagc atgttccggc agataccggt cttggcacgc
     3121 tggcaaaacg ctatctggga cgctacggtc aatggctgac gggcttcagt atgatgttct
     3181 taatgtatgc tctgactgcg gcatacatca gcggtgccgg tgaattgttg gcctccagca
     3241 tcagcgactg gacaggtatt tctatgtcgg caaccgctgg cgtgctgttg ttcacttttg
     3301 ttgccggtgg cgtggtttgt gtcggaactt cactggtcga tttatttaac cgttttctgt
     3361 tcagcgccaa gattattttt ctggtggtaa tgctggtact actgctgccg catattcaca
     3421 aagtgaatct tttaaccctg ccgttgcaac aggggctggc tctgtctgca atcccggtga
     3481 tttttacgtc gtttggtttt cacggtagcg tgccgagtat tgtcagctat atggatggca
     3541 acattcgtaa gctacgctgg gtgtttataa tcggtagtgc gatccccctg gtggcatata
     3601 ttttctggca ggtggcgacg cttggcagca ttgattcaac aacctttatg ggattgctgg
     3661 ctaatcatgc tggattaaac gggctgttac aggcgttacg cgaaatggtg gcctctccgc
     3721 atgttgagct ggcagtgcat ttatttgctg atttagccct cgccacgtca tttctcggcg
     3781 ttgcgttagg cttatttgat tatctggctg atttatttca gcgttcaaat accgttggtg
     3841 gacgcttgca aactggtgca attacgtttc tgccgccgtt ggcgtttgca ctgttttatc
     3901 cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc gctggcggta ctggcattga
     3961 ttatcccttc gctgttgacc tggcaaagca gaaagcacaa tcctcaggcg ggttaccggg
     4021 tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg tggtattgct gtgattggcg
     4081 tgcaattttt gattgcggca gggttgttac cagaagtggg gtgatcagat agcctcaaat
     4141 tccttattgg gtgccagaat taacgctgac acccaatttg gcctcttaat gcaggcagca
     4201 ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg ttacgccccg gtttctcttc
     4261 tgctttgatc ggttgctgaa cagctttttc ctgcggatgc gccatccagt acgcatgtag
     4321 atcaagcgcc gccagtcgaa tggcatctac gctctcttca aacgcttctg gcgacatttt
     4381 ctctacccgc tcgaagtttt cctcagtacc gtgcagcgca atcgcctcca gcgctggttt
     4441 taacgaatcg ggcaacgttg accagtcaga aagtgccacg ccccgcatat agccaaagca
     4501 ccactcctca acaatcgtca gctcgctgcc atcaacttct cgcaagccga ataacggctc
     4561 aaactgctcc gggaattcgt tcagacgctc tgcggtatcg gccatatgtt gaaaagccag
     4621 attcataaag cgcgtcatct ctttctctga cgcccagcgc ggcacatagt cagccccacc
     4681 ccacacggca accagccact gttccggttc aatctcttgc ggagaactca acaccgccgt
     4741 caataaaccg tccagctccg ccacatcaag gatggcgtgg tcagtgttgt atttggtcag
     4801 aatatcgtcc agccattcca actcactttc gtttaacggt cccgttttca tacgcttttc
     4861 cttgtggatc tcaactcgcc agcacctatc ttacatgccg gtccgtatca gagatacttt
     4921 ttgagtggct ttgctggtga ttaaaaatta gggagggtgt aacgacaagt tgcaggcaca
     4981 aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg ctttgatttt attcttatct
     5041 ttcccatggt acccggagcg ggacttgaac ccgcacagcg cgaacgccga gggattttaa
     5101 atcccttgtg tctaccgatt ccaccatccg ggctcgggaa gaaagtggag gcgcgttccg
     5161 gagtcgaacc ggactagacg gatttgcaat ccgctacata accgctttgt taacgcgcca
     5221 aattcttcag gcctttcagc cagacatccg cttgaggccg atgtctttta aactggagcg
     5281 ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta ccaactgagc
     5341 tattcccgca ttcatcaagc aatcagttaa tcacttgatt ttattatcgt ctggcaatca
     5401 gtgccgccgt tcgatgcgtt gcattctact tacctggcgc gatgagtcaa cgatattttt
     5461 caccactttt gatcgtttgc tgaaaattac gccgaaacga tcactgatca agcaaatctg
     5521 cacgcgcagc gctcaaatat tgcaacattg accacagagt cagtaccgca gccacaaaga
     5581 aaagtgcaat accggcgtac tcaacccaaa tgttcggacg ccacagcagc caggccaacg
     5641 ccaccatctg ggcagtggtt ttcactttcc caatccagga gacagccacg ctactgcgtt
     5701 tacccaactc cgccatccat tcgcgtagcg cagaaataat aatttcacgg gcgatcatcg
     5761 ttgccgccgg taaggtcacc caccagctgt gataatgctc ggttaccagc accatggcga
     5821 tagccacgag aactttatct gcgacagggt caaggaaagc accaaaccgg gtactctggt
     5881 tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac cgccgcgacg cagaaaatga
     5941 gcgcggcggc aaacggcgac caggtgacag gcagataaaa gaccaataca aagaatggga
     6001 taaggatgac acggaacagt gtaagcaacg tagggatatt aaattgcata atgacgggta
     6061 actatctgtt gtcagtaaga ttacccctat gttgctacag agacatcaat gtttcaacga
     6121 ccagaagatc ttttctgcca gaccttgcga aatacccggc acttttgcaa tttcctcgac
     6181 gctggcgtta cgtaaacctt gcaaaccgcc catatatttc aacaacattt gccggcgttt
     6241 tggcccgacg ccttcaatgg tttccaggga actggtattt ttgaccttcg cccgtttttt
     6301 acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga atatgctgga taacatgcag
     6361 cgcgggagaa tctggcggca aactgaatcc ctcaccttcc ggctcaaaga acagcgtttc
     6421 cagcccagcc ttacgatctg ctcctttggc aacgccaagt agcagcggat gatttttatc
     6481 ccatgagaca tccagttcgg cgaagacatt tttcgcctgc gcaagctggc ctttgccgcc
     6541 gtcgataagt atcacatccg ggatcttact gtcgtcgatg gctttaccat aacgccgacg
     6601 cagcacctga ttcatcgccg cataatcatc gcccggcgtg atgccagtaa tgttatagcg
     6661 ccgatactcc gcacgcagcg ggccgttagc atcaaacacc acacaggaag cgacggtttg
     6721 ttcacccatg gtatggctga tgtcaaagca ctccatccgc ttcacttccg gcaatttcaa
     6781 cacactggca agtgctgtaa gccgctggtg aacggtagat tgctgcgaaa gtttgctggt
     6841 taaggccgtc gccgcattgg tgcgcgcgag tttcagataa cgcgcccgat cgccgcgagg
     6901 tttggtttga acattaatct tgcgtcccgc cagttctgaa agggaatcgg cgagcagcgt
     6961 tttatcgcta agattaaaat cgagcaggat ctcacccggt aaggtgcgca tctggctgcc
     7021 ttgtaaatag aactgaccta cgaaggtttc taccacctcg ctcagttccg taccgccagg
     7081 cactttcggg aaatagctgc ggctgccgag cactttgccc tgacgaatga acaatacgtg
     7141 gacacaagcc atgcccgcat cgaacgccac accaataacg tcaaggtcgt cgccggtatt
     7201 ggaaacaaac tgtttttcgg tgacgcgtcg caccgcctga atttggtcgc gaatacgtgc
     7261 cgcttcttca aactccagat tctggctggc agtttccata cgactaatga gttgcgtaag
     7321 cacctgatca tctttgccag acaaaaacag gcgcacatac tcgacctgct gagcgtattc
     7381 ttcttcactc accagtcctt caacgcacgg tcccagacag cgccctatct gatattgcag
     7441 acacggacgc gagcgattgc gataaacact attttcgcac tggcgaatgg ggaaaatctt
     7501 ttgcagtagc gccagtgttt cacgtacggc atagccattc gggaacgggc cgaaatattc
     7561 acctttggca tgcttcgcac cacgatgcat cgccagacgc gggtgggtat cgccactcag
     7621 gaagataaaa ggataagatt tatcatcgcg tagcaaaacg ttgtaacgcg gctgatagag
     7681 tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct gtatgagtaa ccgttacatc
     7741 aatttgctgg atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt tgctacggaa
     7801 atagctggaa agccgttttt tcaggtcttt cgctttgccg acatagataa ccgtaccacc
     7861 agcatcgtac atgcgataaa cgcctggctg gctggttacg gtttttaaaa acgcttttgc
     7921 gtcaaactga tcactcactg acttgataat gtctccgcat tacacagacc atggcgaatt
     7981 gccaggtgag tcagctcaac atcgccatga atgtttagtt tactgaacat acgatagcgg
     8041 tagctgttca ccgttttcgg actgagattg agctgttctg agatctcatt gaccttctgg
     8101 cccttggtga tcatcagcat aatctgcaat tcacgttcag acaaactggc aaatgggctt
     8161 tctgtttttt ctggttcgat ctggcttaac gccatttgtt gagcgatgtc agaagcaatg
     8221 taacgctgcc ctgaatagac agaacgaatc gcactcacga cttcctgcgg agccgcgcct
     8281 ttgctgaggt agcccgcagc accggcctgc atgactttcg ctggtaaagg gttttctgta
     8341 tggacggtaa gcatgatgat tttgacatca gctgtggaac gcgcgatttt acgcgtcgcc
     8401 tcaagaccgc caatgcccgg catactcatg tccattagca ccacgtcaac ggcatttgtc
     8461 cggcaccact taacggcgtc ttcaccgcac gatgcctcac cgacgacttt tatacccttt
     8521 atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc aacaagtaga
     8581 acgttgatca aaggaatatc tccagaaata gggataacgc tactgatagt tagtcattcg
     8641 tatattagcg gtttttattg caactttgaa acgttaaaaa tggtgggtat tcgatttttc
     8701 tctcgttttt gtcaattcga ttgtccacag ttatggtcac gcccgtcacc aacggcttac
     8761 cggatcgttt cacagtaagc cctacgatga aatgtgacaa aaattgactt tattcagcaa
     8821 aaatgaaaat cagcccgctg aatatgtaac attaattaac ccgaacaata cgggcaaaaa
     8881 acattcatga taaaaatatt tattggtcat tatattaacg tattttatag cactgccgat
     8941 atcacgctca aaaaacaacc actgctattt ttagcaaagc ttatggtata ctccgccgcc
     9001 ttaacatttt tcaccgcaaa ttttcattgc aacatgacga ggaaaataaa tgagtacgcc
     9061 tgatttttct actgccgaga ataatcaaga actggcaaat gaagtctcct gcctgaaagc
     9121 gatgctgacg ctgatgctac aggcgatggg acaagctgac gcgggccgcg tgatgttaaa
     9181 gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag gctgcagtat tttccaaaac
     9241 ggttaagcaa attaaacaag cctaccgtca gtaatataaa accggctgat agcgtgcctt
     9301 tcggccggtt tttgcatctg gcacgcagga cagaaaagag atcaaattaa gccagtagcg
     9361 gccgcgtaac aggcaacctg ggtcttattt ggtgcattaa tttttttctg catgtttttc
     9421 tggtggaaat tgaccgtatt ctcagagatt gacaaaatca tcgctatctc tgctgatgtc
     9481 ttcccttccg ccgtccattt caaaatttct ttttcgcgct tgctgaaatt catctctggc
     9541 gtcatcacta tttcatcatt taaacgcatc agagccatca gactttcgcg caccagtaac
     9601 tgcattttta attgcagttc atcactaaga atgggtattt cgcgcgcgct gctacgggaa
     9661 aaggacaaaa agcccagcgc ccggtttggc agcattaaat actgagtgac accgcggcgt
     9721 aaaccatgtg cgcgcgcggc ttcccataac ggctgtgctt cgctgaataa gtcatcattc
     9781 cacattaaat ggccctgact aaagttttca gggttcagca ccggatcaat tgcgagaaag
     9841 ttttttgcct gataataact aacccacgcc tcagggtaat tggtgtagaa agccacttta
     9901 ggtcgcgtga atggtaccgg gtggcgaaca cataacgaat agtaatcgta ctctagctgc
     9961 tgagcctgaa gctctatttc atggtagacc tcttctgcgg cctccatcct ctgaaaacgc
    10021 aacagc
//
LOCUS       DAFNBJ010000171        10023 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000171 DAFNBJ010000000
VERSION     DAFNBJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.171"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..378)
                     /locus_tag="L2X49_004114"
     CDS             complement(<1..378)
                     /locus_tag="L2X49_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBN4799116.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHEHKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLD"
     gene            443..1347
                     /locus_tag="L2X49_004115"
                     /pseudo
     CDS             443..1347
                     /locus_tag="L2X49_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1430..1687
                     /locus_tag="L2X49_004116"
     CDS             1430..1687
                     /locus_tag="L2X49_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799117.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            complement(2245..3012)
                     /gene="fecE"
                     /locus_tag="L2X49_004117"
     CDS             complement(2245..3012)
                     /gene="fecE"
                     /locus_tag="L2X49_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBN4799118.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(3013..3969)
                     /gene="fecD"
                     /locus_tag="L2X49_004118"
     CDS             complement(3013..3969)
                     /gene="fecD"
                     /locus_tag="L2X49_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBN4799119.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3966..4964)
                     /gene="fecC"
                     /locus_tag="L2X49_004119"
     CDS             complement(3966..4964)
                     /gene="fecC"
                     /locus_tag="L2X49_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBN4799120.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4961..5863)
                     /gene="fecB"
                     /locus_tag="L2X49_004120"
     CDS             complement(4961..5863)
                     /gene="fecB"
                     /locus_tag="L2X49_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBN4799121.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYTENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(5908..8232)
                     /gene="fecA"
                     /locus_tag="L2X49_004121"
     CDS             complement(5908..8232)
                     /gene="fecA"
                     /locus_tag="L2X49_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBN4799122.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(8319..9272)
                     /gene="fecR"
                     /locus_tag="L2X49_004122"
     CDS             complement(8319..9272)
                     /gene="fecR"
                     /locus_tag="L2X49_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBN4799123.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(9269..9790)
                     /gene="fecI"
                     /locus_tag="L2X49_004123"
     CDS             complement(9269..9790)
                     /gene="fecI"
                     /locus_tag="L2X49_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBN4799124.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
BASE COUNT         2226 a         2764 c         2833 g         2200 t
ORIGIN      
        1 atccagtaag cacggttttg gccttttcgc cattctgttg gctcggttat tagcatcaac
       61 agctttgtaa tagcgtctgc cccgattacg ctgaacttca cgtgagatcg tcgaaggact
      121 gcgattcagc gcagtagcta tcgcacgaat gctcattttg gctgacaaac cagctcgtat
      181 ctcctcgcgc tcagacagtg tcaggtgagc tacagcccgc ttatgctcat ggggttttat
      241 gccgccagta tcccttaaca tagtgaagat cgttccgggt tttgaaccca ggatattcgc
      301 tatttcactg aagcctgttc cgttcttcca tagttcaaaa acagaggctt tttcctctgc
      361 tgtaaatgtt cgtctcattc aaaaaacctc cgcaacccca tgttttcaca taactgttgc
      421 gttgaccaat tgaatctaca accgcgctct tgatgtcaga ctccctgaac agttctcgat
      481 aatcaggaaa ctcagagcgc attatcctgt ggtcacactc tgccatgtgt tcggggttca
      541 tcgcagcagc tacagatact ggaaaaaccg tcctgaaaaa ccagacggca gacgggctgt
      601 attacgcagc caggtacttg aactgcatgg catcagccac ggctctgccg gagcaagaag
      661 catcgccaca atggcaaccc ggagaggcta ccagatgggg cactggcttg ctggcagact
      721 catgaaagag ctggggctgg tcagttgcca gcagccgact caccggtata agcgtggcgg
      781 tcatgagcac gttgctatcc cgaatcatct tgagcgacag ttcgccgtaa cggaaccaaa
      841 tcaggtgtgg tgcggtgatg tgacctatat ctggacgggt aagcgctggg cgtacctcgc
      901 cgttgttctc gacctgttcg caagaaaacc agtgggctgg gccatgtcgt tctcgccgga
      961 cagcaggctt accatgaaag cactggaaat ggcatgggaa acccgtggta agcccgtcgg
     1021 ggtgatgttc caggcgatca aggcagtcat tatacgagca ggcagttccg gcagttactg
     1081 tggcgatacc ggatcaggca gagtatgagt cggcgtggaa actgctggga taacagccca
     1141 atggagcgct tcttcaggag tctgaagaac gaatgggtgc cagcgacggg ctatgtaagc
     1201 ttcagcgatg cagctcacgc aataacggac tatatcgttg gatattacag cgcactaaga
     1261 ccgcacgaat ataatggtgg gttaccacca aacgaatcag aaaaccgata ctggaaaaac
     1321 tctaacgcgg aggccagttt tagttgacca caacagacta cctgaaggga gccgcggtcg
     1381 cctagcagtt gcagtagcag gagatcatcc agccgcagta caggtcacga tgactctggt
     1441 taatgatacc ggctttgacc ccgtattttc cggctctatc gctgaatcat ggcgtcagca
     1501 gccgtgcaca ccatcctatt gttgtgactg ggaggctgcc accatgcttc gcgctttccc
     1561 tctggcgaaa aagggagaag gacgggcccg tctgccttca ctttatgcca gcttcggtaa
     1621 gctgggtgag acaccgactc ataaagatat cattgataac aatcgatcca tcaactggcc
     1681 tgtataacgt ggctgccggt gattaagaaa gctgcaccta cctaagtagt agcaaacgca
     1741 cactttttag aaaaatcgat ggtcagaaac tggattagca attccgttcc aggggtgctt
     1801 ttgatttacg ttggcgtctg atcattgatt tatcctcaaa agcccaacct cattggtaat
     1861 gaaccagctc cgtgaatgtc cgctctggca cagagcgaaa ttttttgatc tccccccctg
     1921 aaatctaaac ttagtcatgt cacgtttttg ggtttctaaa attttaactt cgcgtttttc
     1981 gttgccgtaa gggttataca gaaatgtccg ttaagcagag ttcaaaattg attgccgtga
     2041 tcacgactgg tttgaaagcc gcgcccaagc ctgtacagct ctggtttgcg ttgattatga
     2101 acctgtcagc ctaaagcaag cggatggacg atgagtattg gtaatctttc agagtccgga
     2161 aaagttcagc cccagtctga acaggcttgc tggcgccagt ccagtttcat tcagtcgtgg
     2221 tttggttctt acggcctgtg caatctacct cattaggcac atcggcctgc cagataccgg
     2281 ctcggggtgt atttccgctt ccacgctgaa tactgttctc agcaatcctg gggtcatcac
     2341 ctcttctggt gtgccttgcg ccataacatg tccgtttgcc attaccacca gttgatcgca
     2401 gtaccggcta gcctgattaa ggtcgtgcag cacagcgacc accgttttcc cctgagtccg
     2461 gagttcgccc atcaaccgca tcaggtccac ctggtgattg atatcaagat aggtggttgg
     2521 ctcatcaagt aatacaacgg gcgtattctg ggccaggacc atcgccagaa atgcgcgctg
     2581 gcgctgaccg ccggaaagct cggttaaccg acgaacggca agatgattga tccgggtctg
     2641 gttcatggcg acattaactc gtgcattgtc ttcagcggag agacgccccc agagtgacag
     2701 ccagggatta cgaccatacg aaaccagctc ctggactgtg atcccctctg gcgttaaatg
     2761 gtgctgaggc agcagcgaaa gcctgcgggc caactggcgc gatgagagca tatttatggg
     2821 attatcgccg agaaatacgg tgccagactg cggcattaaa agccgcgaaa aacagtttaa
     2881 cagcgtcgat ttcccgcaac cgttaggacc gatcagggcg gtgatcttcc ccgttggcag
     2941 tgagagtgaa acgtcgttaa gtaccttgtc tgtcccgtaa ctgaccgtca gattttcagt
     3001 tcgtaaagtc atttatcgca ttctcacaag caaccagaca aaccacggcg caccgataat
     3061 ggcggtcagc acgccaaccg ggagctccag tgggggatga ataattctcg ccagcagatc
     3121 ggcaaccacc aacagcaacg cacctgtcag ggctgaaaca ggcagcagtc tgcggtgacg
     3181 tccaccggtg atgctacgca tcatatgcgg caccacgaga ccaataaagc taatcgggcc
     3241 gcaggcggcc acgccggtag atgtcatggc gacagctagt aacaaagccc agaatcgggt
     3301 atggggcacc gacacaccga gcgtggtggc gcgcgcatcg ccgagtgcaa ggaggtcgag
     3361 atcgcggcaa aaactcaggc tcagcggcag aaataaaatc atcagcggga tggcaatctt
     3421 cacaaagctc cagtcacggc cccataagct gccggtcagc cacagcaggg cgttgttcac
     3481 atcctgtggg cgcgagagca tcagataatc cgtcaggctg gcccagcatg cagaaagcgc
     3541 cacgccggtg agcgccagct tcatcggctg gtgggtcttt gccagcatct tcagtaatat
     3601 caaccccgcc atgccgcccg caaaggccag cagcggcagc accatcacgg gcagtgacgg
     3661 cataagaagt agagccccca cagaggccag gctggcggca tggttaacgc cgagaatatc
     3721 cggtgatgcc agagggttgc gcacaatccc ctgtatcagc acgcccgcca cggcgagggc
     3781 tgcaccgaca aacagtgcca gcagcaagcg cggcagtcgg tactccatca atacataata
     3841 atgctcgcgt ccggcctgcc agtcggtcag cagcgcgcgc cacggcacgg ggatcactcc
     3901 catatggagt gataacagcg cacagcccgc cagggcaagg gtgatgaaaa taaccagcgc
     3961 aattttcatc ctcgcctcct cacaagccag acaaagcaag ggctgccaat cagcgccagc
     4021 actgcgcctg cgggcagatc tccggggaag gccagcgcgc gtgcgagtac atctgccagc
     4081 agcatcagcg tggcccccag cagcatgctc actggcagta cgttgcgctg atcgaagcct
     4141 gcccagaagc gcgccagatg tggcaccagc agaccgataa acgccaccgg acctgccacg
     4201 ctgacgcacg caccaaccag aagcagcact aacatattga tgaccaaacg tagcctcgtc
     4261 aggttcactc ccagcgtatg ggcggtgctg tcgctgaggt tgagcaggtt cagttgattc
     4321 gccagcagca acacgacagg gactgcagtg accaccaccg gcaagagctg ccagacatcc
     4381 tgccagcggg cgtgggacac tccgcctgcc agccagtaaa agatgccgta agcatgatct
     4441 tcggccagca gcagggtgat gcgggtaagg cccatacaaa aggccgacag cgcgataccc
     4501 gcgaggatca gtttgtttct gtcatgggta tgacgaaatc cgcctcctgc ggtcatgacc
     4561 agcagccagc tcacgccgcc cccgcatgcc gcgatgaacg acagagaata gcctgcaatc
     4621 ggcgtcggac tcagcgcgct ggtaagcgcc atagccagcg ccgcgccgct gttaatgccg
     4681 agcagtgaag gagaggccat tgggttgtgg gtcagggttt gcagcagcgt gcccgcgagc
     4741 gccaggcttg cgccgatcag aacggcgacc aggcttcgtg gcaaacgaag gttttgcacc
     4801 agcgcttctg gtagcgttgg cgtgtgtcca ggcagcaggg cgcgggttgc atctgctccg
     4861 gaaacaggaa tggccgagta gcaaaacaga ctcagccaga aaataataat aagtgctgca
     4921 acgggaagcc cccacagcag caccgggtgt tttatcgcgg tcatttcaca acggtaagcg
     4981 gctgatggtg gaagattttt accgtgtcag cggcaatacg ctctgcagca aaaataccgc
     5041 gcatccgcgc ccaggtgtta ctgtcgaccg aagcaacctg ctgcttctgc gcggcggtta
     5101 acatctgcca gagcggatct tgttgccagc gtttaacaat gctctcttcg cgatagtggg
     5161 caaccagcag ccaggcagga ttgaccgcca gcagttgctc caggccgatg gacggcatgg
     5221 acgcacccgc catcgcagcg ggaacgttca gccccagaga ggccagcacg ctgccggtcc
     5281 aggtctcctg agtatgcagg ttgaattgct gttcgcgtga tgtgccaaag gccacgcgtg
     5341 tccctttggg aagctggctg gcccactgcg ccatcctctc tttatgttgt tccaggcgtg
     5401 cctgcatctc tcgcttttta cccaccattt cgccgatgat agccgcagat tgcaaatttt
     5461 cagtgtaggt ttcgttgcgg gacttaagca gcagtaccgg cgcgatttgc tgcaaggcga
     5521 tgtaaacccc cgcatggcga ctgctgtcgg caatgatcag gtctggtttc agagcggcaa
     5581 tggcttccag gctcggctgc gcgcgcgttc cgacggactg ccacggtttc aggtgcgcac
     5641 gcacttcggg caggatgcgt tttgcatcgt tatcgtcggc aataccgatc gggctgacgt
     5701 ccacggcggc cagcgcatcg gcgaacgaga gttccagcac cacaatccgt tgtggcgttt
     5761 tttcgagtgt aaacgtgccg tgttcgtcct gaaccgtggc ggcaaaggcg tggctgatca
     5821 ccagcagcag gcctgcaaaa acaaaacgga taaatgccaa cataatcaca ttccagctaa
     5881 aagcccggca agccgggcgt taacacatca gaacttcaac gacccctgca tatacagcgt
     5941 gcgcggctgg cctgcataga tgcctttgtt gttgtcgtca taagagcgga tgaagtagtc
     6001 ctggtcgaag atgtttttca caccgaatgc caggttcaga tctgccatct gcgggccaaa
     6061 atcatacgcc acgcgtgcgc cccagagcat gaagccggga atgcggccgg tactgccgtc
     6121 ggcgctctct ttcaccgtat tggcgttatc cgcaaactgg ctggactgga aatcgctgtt
     6181 cagattgaac gtccagtttc ctggcttgta gtccacgccc agcgtgcctt tatgtttcgg
     6241 ggagaatggt accagattgc cgtaggtgtc gcctttctcg cggatttccg cgttcacata
     6301 cgcatagctg gcgtagatgg aaacgttatc aagcgttggc gttagcgtac ccagatcgta
     6361 acgtgcctgc gtttccagcc cggtatggcg cgttttgcca cgtgcagtga cggtgtcgtt
     6421 ggtctggttg gagtcgtact gattgttaaa gttaatcagg aacagcccca tttccgccgt
     6481 cagcgcgccg tcgtcgtagc gggtaccgag ttcccaggtt cgcgcttttt ccggttccac
     6541 attgccgctt tgcacagcct tgccaatctg gctgtactgt acggtgccga acgagccttc
     6601 agtgtttgca taaagattcc agctgtcagt caggtgatag agcacgttca acgccggaag
     6661 cggtgcgtta tagctcactt cttcgtgcgt gcctgtgatg gcgttgttct ggtatgactc
     6721 gatatgttcg aaacgcatac ccggcgtgat ggtccagttg ccgatgtcga ttttgtcatc
     6781 cagataccag gcgtgcgcct cggtgccgga acgcgtatcg cggtcgtaag ggcttgagcc
     6841 ggacggcaac tgcccgctgc tggtggcggt gtagtaacgc atttcatgcg ttgattcatt
     6901 caaatagcga tagcccacgc ccacttcgtg cgcggaaggg ccgatcataa agatctggct
     6961 gtagcgtggc tcaataccgc gcacccagta gttacgcggc gagagggtga tgcgtttgcc
     7021 ttgctccagg tagccgctgc gcagggtttg ggtgtagaac ccctgaatgt tgaatttatg
     7081 ctggctgtct ggctggaact ggtagcccag gctcgccagc ttgcgacgac cccagaagcg
     7141 gtcatacggg cgggtggatt gccagcgatc ggcgtcgtaa tccgcgcgag acaggccacc
     7201 gggcatgtcg gcttcaccat cgtaatattg cagcaggctg ttgaaggtgt gcacctcatc
     7261 cggcgcatat ttgcttttca gcatcaggtc gtcgatgcgg gtggcgctgt gctcgcgcca
     7321 gtcactgccg cgcgtgccgg agtagagcag cgcggtgcca aaaccgttgt ccgctgtgcc
     7381 gcccaccatc aggttgtgcg tctctttcgg gttgttttgt gaagaggttg ggctgagctg
     7441 accttccacg cccgcctcga taccaaagtc ctgcggaatg gcacgggtaa caaagttcac
     7501 cacgccgccc acgctctgcg gtccgtaacg caccgcacca ccaccgcgta ccacgtcaat
     7561 ggcatccatg ttgccgagcg aaacgggagc cagtgaaagc tgcggctgac cgtaaggggc
     7621 gaaggggacg gggatgccgt ccatcaggac ggtcgagcgg ctggcgaggc gcgggctcag
     7681 gccccggatg ccaaagttca tcgccaggtc gtggctgccg gtgccgttgt tttccggcgc
     7741 gctgacgcca gggatgcggt taagtacctc acgcatggtg gttgcgccgg ttttggcgaa
     7801 atcctcacgg cggatcacgt cacgcgcgcc agcatgttca aatacgtcgt tttcacgcgc
     7861 atcacccagc cagtcgccga ccacggtcag ggcatcttct tttggtgcgg gcgcgggctc
     7921 cagcgtccag ctgttatttc ccagcggttt tacctgcagt ccgctgccgt ccagcagttg
     7981 ttgcaggccg ctctcgacgt cgtaatcgcc gtgcaggccg ttgctctgct tgccgcgcgt
     8041 caggctggcg tcaaccgaga gggtaaatcc gctgtgtgcg gcatactgat tgagcgcttt
     8101 atcgagcgat cccggtgcga tattaacctg tgcagcaaaa gcggaaaacg agagaccggc
     8161 cagcggcagc aggctcaggc gaatggcgtt aaccaaaggt gttgttttac gaaaaacgcg
     8221 taacggcgtc ataccttccc catcatcatt tttgttgtgt tcagctatga gtcgaacgag
     8281 aagggtaaaa aggacaatcg aaataagaat tattttcctt acagtggtga aatgtttatc
     8341 cagtaccgcg taatagactg aattttaacg ggaagcgttt gcgcgataac gttcaggatc
     8401 gcatcggtat ttttcagcgg gaacgtcccg ctcaggcgca gcccggcaac ggcgggatcg
     8461 cagcgcagca cgccgttgcg gtaacgggtt agcgtggcta tcacctcacc cagcggttta
     8521 tcgctgaagc tcaggatgtc cttcgtccag cttgtactct cgtcatccag cggtttcact
     8581 gcgccaaact cagaggcgct gaactgcagg ctttcaccag cgttcacgat gcgtttttgc
     8641 gcgggggcac tggcgagaag cacttccaca gcgtgctgct gcacgtcaag ctgcgtgaaa
     8701 ttatcctgct ggcggacggt aaattctgtc cctaaagcag tgagctggcc ctgacgggtc
     8761 aggacacgga aggggcgttg cagggcatct ttcgcggtag taatggcgat ttcaccgtac
     8821 cagagccgga cggtgcgctg atgcgcatca aaacgcacat ccgccgcgct ttgggtattc
     8881 agcgtgagca gggagccatc ttccagttgc tgacggctga ctgtgccttt ggcggtgcgg
     8941 taatctgccc gcagaccttc gccggtttcc gactgccaga gctgccagcc tccgccagcg
     9001 ccgagcaaca gcagtaatcc tttcatcacg tgacggcggg tgaggcgggt atcgtgcaac
     9061 gccctgctgg caacgtcgcc aggcacaccg ccaagctggt tgcgcaggtt ttcaacctgc
     9121 tgccaggccc actggttatc ctgatcctgt tcataccact gttgccagcg cgcttcctgt
     9181 tgtgggctga cgcgctcgcc gcttagcacg gcataccagt gggaagctga acgcagcgcc
     9241 tgacggcggg aatcggttaa caaaggattc ataacccata ctccagacgg aacagcaggc
     9301 agtgctcgac ggctttcgcc acgtattttt tcacggagct gatggaaaca ccgagtttgt
     9361 gcgcaatctc gctgtatgtc agaccatcca gttgcgaaag cagaaacgct tcacgtgttt
     9421 tgccgtttag cccgtccagc atgctgtcga ggagttgtag ggtctcgagt tggctttcgc
     9481 gttcctcagg tgaaggcgct cccccctccg gcataagcgc cagcatctcc agatacgctt
     9541 tttccagcgc gtttcggcga aacaggtcca ccatcacgcg tttggcgata gtgcagagga
     9601 aggagcgagg atcgcggatc gtcgagagcg tttcgctgac cattacccgc aaaaaagtgt
     9661 cctgggcaat gtcatctgca tcaaaagcag actggagttt gcgcgtcagc cagcttttca
     9721 accagccgtg atgtgtgcca taaagcgact cgaacgttaa ggaagctgtg gtagtggcgc
     9781 ggtcagacat gcggagtgca tcaaaagtta attatcacgt agtcatatta atatgagaat
     9841 ggttatcatt acaattggaa ataaaattgt ttccaataga catttttaac atgttgtttt
     9901 tctaagtgtt ataaggtagg tataaaatgg gatggagcct ctgcttctgg catgtgtcgg
     9961 tcagaatgac tcatgatgtg gtctgctatt attgacatcc tcactgccct aaaggatggg
    10021 gat
//
LOCUS       DAFNBJ010000172         9925 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000172 DAFNBJ010000000
VERSION     DAFNBJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.172"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            39..1304
                     /locus_tag="L2X49_004124"
     CDS             39..1304
                     /locus_tag="L2X49_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBN4799125.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(1545..3137)
                     /locus_tag="L2X49_004125"
     CDS             complement(1545..3137)
                     /locus_tag="L2X49_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBN4799126.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            3356..4276
                     /gene="ldtB"
                     /locus_tag="L2X49_004126"
     CDS             3356..4276
                     /gene="ldtB"
                     /locus_tag="L2X49_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBN4799127.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(4335..5453)
                     /locus_tag="L2X49_004127"
     CDS             complement(4335..5453)
                     /locus_tag="L2X49_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBN4799128.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(5450..5917)
                     /gene="mntR"
                     /locus_tag="L2X49_004128"
     CDS             complement(5450..5917)
                     /gene="mntR"
                     /locus_tag="L2X49_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBN4799129.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            6103..6231
                     /gene="mntS"
                     /locus_tag="L2X49_004129"
     CDS             6103..6231
                     /gene="mntS"
                     /locus_tag="L2X49_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBN4799130.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            6503..8086
                     /gene="opgE"
                     /locus_tag="L2X49_004130"
     CDS             6503..8086
                     /gene="opgE"
                     /locus_tag="L2X49_004130"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBN4799131.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(8135..8650)
                     /gene="ompX"
                     /locus_tag="L2X49_004131"
     CDS             complement(8135..8650)
                     /gene="ompX"
                     /locus_tag="L2X49_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBN4799132.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(8703..8768)
                     /locus_tag="L2X49_004132"
     CDS             complement(8703..8768)
                     /locus_tag="L2X49_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799133.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            9003..9890
                     /gene="rhtA"
                     /locus_tag="L2X49_004133"
     CDS             9003..9890
                     /gene="rhtA"
                     /locus_tag="L2X49_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBN4799134.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         2433 a         2621 c         2437 g         2434 t
ORIGIN      
        1 tcagaatgat ttcgaatgaa agttaatgag aggtggttat ggcttctact tttaccagcg
       61 acacattgcc tgccgatcac aaagcagcta tccgtcagat gaagcacgcg ctgcgggcgc
      121 agcttggcga cgtccagcag atctttaatc agctaagcga tgacattgcc acgcgagtgg
      181 ctgaaatcaa cgcactcaaa gcacagggcg atgccgtctg gccggtgctg tcttatgccg
      241 atatcaaagc aggtcatgtt actgcagagc agcgcgaaca gattaaacgt cgcggttgtg
      301 cggtgataaa aggccatttc ccccgcgaac aagcgctagg ctgggatcag tcgatgctgg
      361 actatctgga ccgcaaccgc tttgacgagg tctacaaagg ccccggcgat aatttcttcg
      421 ggacgctcag cgcttcacgt cccgagattt accccatcta ctggtcgcag gcgcaaatgc
      481 aggcccgcca gagtgaagaa atggcgaatg cgcagtcgtt tctcaatcgt ctgtggacat
      541 ttgaaagtga tggaaagcaa tggtttaacc cggatgtgag cgtcatctac cctgaccgta
      601 tccgccgccg tccgcccgga acgacctcca aaggtcttgg agcgcatacc gactccgggg
      661 cgctggaacg ctggctgctt ccagcgtatc agcgcgtttt cgccaacgtc tttaatggca
      721 atctggcgca atatgatccc tggcatgcgg cacatcgtac ggaagttgaa gagtacacgg
      781 tggacaacac caccaaatgt tccgtgtttc ggacattcca gggctggaca gcgctctctg
      841 atatgctgcc tggtcagggg ctgctgcacg ttgtgcccat tcctgaagcc atggcgtacg
      901 tactgttacg tccgctgctt gatgatgtgc cggaggatga actgtgcggc gtagcgcccg
      961 gaagagtgtt gccggtatca gagcaatggc atccactgtt aattgaggcg ttaaccagca
     1021 ttccaaaact cgaggccgga gactccgtct ggtggcactg cgacgtcatc cattccgttg
     1081 cccccgttga aaatcaacag ggttggggca acgtgatgta cattcctgcg gcaccgatgt
     1141 gcgagaaaaa tcttgcctac gcgcacaagg tgaaggccgc actggaaaaa ggcgcatcgc
     1201 cgggcgactt cccgcgcgag gactatgaaa caaactggga aggacgcttt acgcttgccg
     1261 acctcaacat tcacggtaag cgagcgttgg gcatggatgt ttgagagtta atggaagtga
     1321 attgatgtgg gattaagaaa atcgattcga cgttactcag attgctgaca agtgcgcttt
     1381 cttaatgcct gatgcggcgt taacgcctta tctggcctac aaagtcctgc agattcaata
     1441 tattacaggt actgcgtagg cctgataagc gtagcgcatc aggctatttt gcatttgtca
     1501 tcagccgccg tcaccccgac agtatcgggg tgacggataa tcacttactc gatcccttta
     1561 ctacgcaggt aatcttcgta attaccgcta aagtcgatca cgcgttccgg ggtgatttcc
     1621 agaatgcggg tcgccaggga gcttacgaac tcacggtcgt gtgaaacaaa gatcagcgtg
     1681 ccctgataca gttccagcgc catgttcagt gactcgatgg attccatatc caggtggttg
     1741 gtcggttcgt ccatgatcag aatgttcggc ttctgcatca ttaacttacc aaacagcatc
     1801 cgcccttttt ccccaccgga aagcacttta gctggctttt tgatgtcgtc ctggctgaac
     1861 agcaaacgac cgagaatgct gcgtaccgcc tgctcgtcat cgccttcctg cttccactga
     1921 ctcatccatt cgaacacggt cagatcattt tcaaactcat attcgtggtc ctgggcgtag
     1981 taaccaatgc gcgcgttttc agaccatttt acggtgccgc tgtccggttg cagatcgccc
     2041 accagcgttt tcagcagcgt tgatttaccg acgccgttgg tacccagtac cgcaagtttt
     2101 tcaccgacct ccagcagcag gttgagattt ttaaacagtg gaccgttatc aaaccctttg
     2161 gtcagacctt ccacttccag cgcgttacgg aacagtttct tatcctgttc aaaacggatg
     2221 aacgggttct gacggctgga ggctttcacc tcttccagtt tgattttatc aatctggcgc
     2281 gcgcgcgaag ttgcctggcg agatttcgag gcgttggcgc taaagcggct aacgaaagat
     2341 tgcaactcag caatctgcgc tttcttcttg gcgttatcgg ccagcagacg ttcacgcgcc
     2401 tgggtcgccg ccgtcatgta ctcatcgtag ttacccggat aaacgcgcag ctcgccgtaa
     2461 tccagatccg ccatgtgcgt acagaccatg ttaaggaagt gacggtcgtg cgagatgatg
     2521 atcatggtgc tgtcacgctc gttcagcacc tgttccagcc agcgaatggt gtcgatgtcg
     2581 aggttgttgg tcggttcgtc gagcaggaga atatccggat cagcaaacag cgcctgcgcc
     2641 agaagcacac gcagcttcca gccaggagca acttcactca tcgggccgta gtgctgttcc
     2701 actggaattc ccacgccaag caacaattca ccggcgcgag cttccgcaga gtaaccgtcc
     2761 atttcgccgt atttaacttc cagatcggcc actttatagc cgtcttcctc actcatttcc
     2821 ggcaaagcat agatgcggtc acgctcctgc ttcacttccc acaactcttt atgccccatg
     2881 atcaccgtat ccagcacagt gaactcttca aaggcaaact gatcctgacg cagcttacca
     2941 atgcgctcgt tgggatcgag ggaaacgtta cccaaagtcg gctctaagtc gccgccgagg
     3001 atcttcataa aggtggattt accactaccg ttcgcgccaa tcaggccgta acggttgccg
     3061 ccgccaaatt tgacggaaat gttttcaaac aacggcttac tgccgaactg catggtgacg
     3121 ttactggaaa ctaacacagg cgtatcctga aaagagatat gacaaaccgc gcattatgcc
     3181 ataagtctga aagaagatcc tgctcctcgc ttaaggtcta cactcaagcc aaaggcaaaa
     3241 aatgtgattt cgtacacatc tgatttcact gtgagctgga atgaacttat aatgcgcttc
     3301 caatactcta atattctcaa tccaatggcc tgccaggcac aaaatctcgc ttaacatgaa
     3361 tatgaaattg aaaacattat tcgcagcggc cttcgctgtt gtcggctttt gcagtaccgc
     3421 ctctgcggta acttatcctc tgccaaccga cgggagtcgc ctggttggtc agaatcaggt
     3481 gatcaccatt cctgaaggta acactcagcc gctggagtat tttgccgcgg agtaccagat
     3541 ggggctttcc aatatgatgg aagcgaaccc gggtgtggat accttcctgc cgaaaggcgg
     3601 tactgtactg aacattccgc agcagctgat cctgccggat accgttcatg aaggcatcgt
     3661 cattaacagt gcagagatgc gtctgtatta ctatccgaaa gggaccaaca ccgttatcgt
     3721 gctgccgatc ggcattggtc agttaggcaa agatacgcct atcaactgga ccaccaaagt
     3781 tgagcgtaag aaagcaggcc cgacctggac gccgaccgcc aaaatgcacg cagagtaccg
     3841 cgctgcgggc gaaccgcttc cggctgtcgt tccggcaggt ccggataacc cgatggggct
     3901 gtatgcactc tacatcggtc gcctgtacgc tatccatggc accaacgcca acttcggtat
     3961 cggcctgcgt gtaagtcatg gttgtgtgcg tctgcgtaac gaagacatca aattcctgtt
     4021 cgagaaagta ccggtcggta cccgcgtaca gtttattgat gagccggtaa aagcgaccac
     4081 cgagccagac ggcagccgtt atattgaagt ccataacccg ctgtctacca ccgaagccca
     4141 gtttgaaggt caggaaattg tgccaattac cctgacgaag agcgtgcaaa cagtgaccgg
     4201 tcagccagat gttgaccagg ttgttcttga tgaagcgatt aaaaaccgct ccgggatgcc
     4261 ggttcgtctg aattaatctt caaaacttaa agcaaaaggc ggactcataa tccgcctttt
     4321 ttatttgcca gaccctagtt ggccgggagt ataactaaca aaacatatcc caccaacgcc
     4381 gcccacaaca gcatcggtat tgaatagaga tggaaacgcc accagatgcg gcgatcgttc
     4441 gccatacgta gcgcaatcaa atttgccagc gatccgggta ataacccaaa gccacctacg
     4501 tttaccgccc ataccagtaa taaagacggc ggcacatagt tcagcaacaa tatggtactc
     4561 ggcacgttac tgatcacctg cgataaaccg attgccgtta accataaccc gggttcagat
     4621 agatgactca cgttacccaa cacgccttgc aacgctggaa gctgggtcag taaatggacg
     4681 tcgataaaca tcgccataaa caccagcagc agcgtccagt ccacactgag caccacgcga
     4741 cgtgccagca gcgcaaagcc tgccgccaca atcaccagtc cccacagctc ttgtttgaac
     4801 tccagcgccg tcagaaagac gatatacagc cccagacaac tccacaccag ccgcggtttc
     4861 cactccggtg tttgcacccc cgtatggtat tgcagcgcct ttccagggaa acaacaccag
     4921 cacaggagca ggagcgtcag catcattgcg ccagccagcg gtgccatttg ggcaataaat
     4981 ccggcaaacg aaagaccaga acgtccccag ataagaatat tttgcgggtt gccaattggc
     5041 gtcagtagcg aaccagcgtt gactgccagc gcctcaaaaa taatcagccg attaaccggg
     5101 atctcacaca gtctttttag cgtgatagtc agcggaacaa caataaacag cgcgacatcg
     5161 ttggtcagaa aggtagaaag cagcgccgcc gccagcacca taaacatcgc cagccgacgc
     5221 tccgtagcaa agcggcgcac cattttgcgc cccagcacat caaaataacc gcttaactcc
     5281 acacctttgg tcagcagcat caggccgctt aaggtgatga tggtgtgcca gtcgatagca
     5341 gcaggccagg atttcggtgc aaagggcacg aaaaagctta atccgatacc aacaagaatt
     5401 aataactgaa aaaaacgatc gccttgcagc gtgcgtaaaa aaggcaggct catttggcac
     5461 cgtgtttctg ggtaaacaaa cgaaaagcgt cgagcgtctc ttcactaaca tggtgctcca
     5521 tgccttccgc gtcgcgacgg gcgatttccg gactgacgcc caacaccaac aagaaatttt
     5581 cgactatctg atgtcgctcg cggctttcct gcgccagctt ctctccttct gccgttaaaa
     5641 acacgccacg ccaggggatc atttcaatca gccccatggt tgccagccgc ttaagcattt
     5701 tagccaccgt cggttgcgaa actcccagac gagcagccat gtccacctga cgagcttccc
     5761 ccacttccct gatcaagtca gaaatcagct caacgtaatc atcaataagc tcgcgccgat
     5821 gcgcctctct gacctggcgg aacccttcaa cgtgctcttc cacgttcact aactgcgtca
     5881 ctttttttgc tgttggcgta cctgcgcgac gactcattgt gcttcctcat tatggtgacg
     5941 catacatagc gttctcattt cgtgcgcaca ttgtaaacca gagttgcgaa ggtacaaaaa
     6001 attaacgttt tagcaatagc tatataatat agcctgtgct atatctgtat gtaatgcaat
     6061 catccctcaa ggatcgacgg gattagcaag tcaggaggtc ttatgaatga gttcaagagg
     6121 tgtatgcgcg tgtttagtca ttctcccttt aaagtacggt taatgctgct ctctatgttg
     6181 tgcgatatgg tcaacaacaa accgcagcaa gataaacctt ccgataaata gcggcgtcgc
     6241 ggtacgccgc ttcactcctg ctttcatgca ggcataacgc gttttggtct gaaaaacccc
     6301 actttttgtc ggatttgcaa tccccttcgc aaaagatttg ttcgtcagta gttgacctga
     6361 acggcggctc gctctatctt cttgcagccc tgcgtatatt gcggctcgcg gatgcggacc
     6421 cctttccact cttcacgcac tcttgcaggt attgaccctt gacgccaggg taagcacatg
     6481 gcgtttgtta cgatagtggc atatgaattt aaccctcaaa gaatcgcttg ttacccgtag
     6541 ccgggtattt agcccgtgga ctgcgttcta ctttttacag tcgctattaa ttaacctcgg
     6601 cttaggttac cccttcagtt tgctctacac cgctgcgttt acggctattt tgcttttgct
     6661 atggcgaaca ttgcctcgcg tacaaaaagt tctggtcggt gtcagttcgc tggtggcggc
     6721 ttgttatttc ccttttgctc aggcctacgg cgcgcctaat ttcaatacat tgctggcatt
     6781 gcactccacc aatatggaag agtcgaccga aatcctgacg atttttccgt ggtacagcta
     6841 cctggtcggc ttatttattt ttgcgctcgg cgtaatagca atcaggcgaa aaaaagagaa
     6901 tgaaaaagcg cgctggaata ccttcgacag cctgtgtctg gtattcagtg tggcgacatt
     6961 ttttgttgct cccgtgcaaa acctggcctg gggtggcgta tttaaactga aagatactgg
     7021 ctatccggta tttcgttttg ctaaggatgt catcgtcaat aataacgagg tgattgaaga
     7081 gcaagaacgg atggcaaaac tttccggaat gaaagatacc tggacggtca ctgccgttaa
     7141 gccgaagtat cagacctatg tggtggtgat cggtgaaagc gcgcgtcgcg atgccctcgg
     7201 tgcctttggc ggtcactggg acaatacccc gtttgccagc agcgttaacg gtttgatatt
     7261 tgctgactac attgccgcca gtggctccac gcagaaatcg cttggcttaa cgctcaatcg
     7321 cgtagtcgat ggcaaaccac agtttcagga taactttgtc accctggcaa atcgcgcggg
     7381 cttccagacc tggtggtttt ccaaccaggg ccaaatcggc gaatacgata ctgctatcgc
     7441 cagcatcgcc aaacgtgcag atgaagtgta cttcctgaaa gaaggtaatt ttgaagcaga
     7501 taaaaacacg aaagacgaag cgttactgga tatgaccgct caagtgctgg cgcaagagca
     7561 ctcgcaaccg cagctgattg ttctgcatct gatgggctcg catccgcagg cctgcgacag
     7621 gacacaaggc aaatacgaaa cctttgtgca atcgaaagaa acgtcgtgct atctctatac
     7681 catgacgcaa acggacgatt tactgcgcaa gctgtacgat cagttacgca acagcggcag
     7741 cagcttctct ctggtttact tttctgacca cggtctggcc tttaaagagc gcggcaaaga
     7801 cgtgcaatac cttgcccatg atgataaata tcagcaaaat ttccaggtgc cttttatggt
     7861 catttccagc gacgataaag cgcatcgggt gattaaagcc cgccgctcag ccaatgactt
     7921 cttaggcttt ttctcgcagt ggacgggaat taaagcgaag gaaataaata tcaaataccc
     7981 gtttatatct gagaagaaag ccgggccgat atacatcacc aacttccagt tacagaaggt
     8041 ggattacaac catctcggaa ccgatatttt cgacccgaaa ccttaaaaac aaaaatccgc
     8101 cccgagaggc ggatttttta tatcaccaaa gtgattagaa gcggtaacca acaccggcaa
     8161 tccaggtgcc tacgtcaacg ctacgaatac ggctctgctc gtaagagaag tccagagcaa
     8221 cgttttccat cgggttgaac tgcagaccag caccgtagga gaaaccgtag tcgctggtgt
     8281 cgtgtttgta ggtcgggtat tcagtggtct ggaatttacc ataacccaca cccactacac
     8341 cgtagatgct tgcccagtcg ttaatgcggt aagccggacc agcagtgatg ccgtagtact
     8401 ggtttttgtt gtagtcacca gagcttgcag tacggctttt ctcggtgtaa gtgaaagaac
     8461 cgatcacacc cagcgggctg ttgtcttctt catagcggta tttcaggttg aaaccgccca
     8521 ttttgttcat ttggccctga gcgtcgctct gtgcgtaacc gccagttacg gtagaagtcg
     8581 cagctacgga agtacctgcg gtgaaagcca gaactgcggc cagtgctgaa agacatgcaa
     8641 tttttttcat aaccacctca aatgtgattc aaataagtcc taagttttaa atatatcaaa
     8701 aattaatggg aaactcttcg cgatttgtga tgtctaacgg gccatttcat gtaacagaac
     8761 gtttccatac accgctatcc atctaaattt aaatcacttt ttcagagaac tgcgtaagta
     8821 ttacgcatgt tttccctgtc attcatccag attattccta atcaccagac taatgattcc
     8881 atcaatcctg gcgcatttta gtcaaaacgg gggaaaattt tttcaacaaa tgctcaacca
     8941 gcattgggta tatccagtac actccacgct ttacttaagt ctagatattt gtgggagaaa
     9001 ggatgcctgg ttcattgcgt aaaatgccgg tctggttacc aatagtcata ttgctcgttg
     9061 ccatggcgtc tattcagggt ggagcctcgt tagctaagtc actttttcct ctggtgggcg
     9121 caccgggtgt cactgcgctg cgtctggcat taggaacgct gatcctcatc gcgttcttta
     9181 agccatggcg actgcgcttt gccaaagagc aacggttacc gctgttgttt tacggcgttt
     9241 cgctgggtgg gatgaattat cttttttatc tttctattca gacagtaccg ctgggtattg
     9301 cggtggcgct agagttcacc ggaccactgg cggtggcgct gttctcttct cgtcgcccgg
     9361 tagatttcgt ctgggttgtg ctggcggttc ttggtctgtg gttcctgcta ccgctggggc
     9421 aagacgtttc ccatgtcgat ttaaccggct gtgcgctggc tctgggggcc ggggcttgtt
     9481 gggctattta cattttaagt gggcaacgcg caggagcgga acatggccct gcgacggtgg
     9541 caattggttc gttgattgca gcgttaattt tcgtgccaat tggagcgctt caggctggtg
     9601 aagcactctg gcactggtcg gttattccat tgggtctggc ggtcgctatt ctctcgaccg
     9661 ctctgcctta ttcgctggaa atgattgccc tcacccgttt gccaacacgg acatttggta
     9721 cgctgatgag catggaaccg gcgctggctg ccgtttccgg gatgattttc ctcggagaaa
     9781 cactgacacc catacagctg ctggcgctcg gcgcaattat cgccgcttca atgggttcta
     9841 cgctgacagt acgcaaagag agcaaaataa aagaattaga cattaattaa atttacattt
     9901 ctgcatggtt atgcataacc atgca
//
LOCUS       DAFNBJ010000173         9822 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000173 DAFNBJ010000000
VERSION     DAFNBJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.173"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            25..597
                     /locus_tag="L2X49_004134"
     CDS             25..597
                     /locus_tag="L2X49_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HBN4799135.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            645..1376
                     /locus_tag="L2X49_004135"
     CDS             645..1376
                     /locus_tag="L2X49_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBN4799136.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1401..3923
                     /locus_tag="L2X49_004136"
     CDS             1401..3923
                     /locus_tag="L2X49_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBN4799137.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3934..5007
                     /locus_tag="L2X49_004137"
     CDS             3934..5007
                     /locus_tag="L2X49_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4799138.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            5255..6295
                     /gene="pstS"
                     /locus_tag="L2X49_004138"
     CDS             5255..6295
                     /gene="pstS"
                     /locus_tag="L2X49_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBN4799139.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6382..7341
                     /gene="pstC"
                     /locus_tag="L2X49_004139"
     CDS             6382..7341
                     /gene="pstC"
                     /locus_tag="L2X49_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBN4799140.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7341..8231
                     /gene="pstA"
                     /locus_tag="L2X49_004140"
     CDS             7341..8231
                     /gene="pstA"
                     /locus_tag="L2X49_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBN4799141.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8322..9095
                     /gene="pstB"
                     /locus_tag="L2X49_004141"
     CDS             8322..9095
                     /gene="pstB"
                     /locus_tag="L2X49_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBN4799142.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9110..>9822
                     /gene="phoU"
                     /locus_tag="L2X49_004142"
     CDS             9110..>9822
                     /gene="phoU"
                     /locus_tag="L2X49_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBN4799143.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGK"
BASE COUNT         2523 a         2261 c         2504 g         2534 t
ORIGIN      
        1 atatattata tcaatggagt ttttatgaag cgtaatatta taggcggtgc attcactctg
       61 gcatctctaa tgctggccgg gcatgcactg gcagaagatg gtgttgttca cttcgtcggt
      121 gaaattgtcg acactacttg tgaagttacc tccgatacag ccgatcaaat tgtcccactg
      181 ggtaaagtca gtaaaaatgc attttcaggt gtaggtagtc tggcgtcgcc acagaagttc
      241 agtattaaac ttgaaaattg cccggcaacg tacactcaag cagccgttcg ttttgatggt
      301 acagaagcgc ctggcggcga cggcgacctg aaagtgggta cgccgcttac agcaggcaac
      361 cctggtgatt ttaccggtac aggacaagcg attgcggcaa ccggcgttgg tattcgtatt
      421 tttaaccagt ccgataattc gcaggttaaa ctttataacg actctgctta taccgctatc
      481 gatgctgaag gcaaggctga aatgaagttt attgcacgct atgtggcaac caatgcgacc
      541 gtaacggctg gtacggcgaa cgctgattca caatttactg tcgaatataa gaaataattt
      601 atcaagggcc tttttattca gttggctttg gccagggagt cgttatgtcc agaaaaagtt
      661 gtttaatcag gctctttaaa acaacagttt catgtaagtt atttaccgcg attatactca
      721 gcgttttttt aggccaacca gcattaactt atgcaggcgt tgtcattggt ggtacgcggg
      781 tggtttactt aagtaataat gctgataaat ctatttctgt atttagcaaa gaagaaaaaa
      841 taccttatct catccaggct tgggtagatc catttaataa agaggataaa agcaaagcgc
      901 cgtttaccgt tattccgcca gtatcacgat tagaaccttc gcaagaaaaa atattacgca
      961 ttattcacac caaaggtgtg tcattgcctg atgatcgcga gtcagtcttc tggctgaata
     1021 ttaaaaatat tccgccatca gcgagcaaca aagcgacgaa ctcgctggaa atagcagtaa
     1081 aaacgcgtat caagctgttc tggcgaccag caaatatccg ccttattccc gaagatgctg
     1141 cgccgaaagt gaaatggcgc cgggaagggc gaaatctgat agcagagaat cccaacccaa
     1201 ttcatatcag tgtgatggat gtcatcgttg atggccatga tgtgcccctc aatatgatcc
     1261 gtccttttga aacgctcacg ttacctttac ctgctaattc tgcgggaggc caaatgacct
     1321 ggcgatttat taatgattac ggcgcagtca gtgacccgat aaaaatgacg ctgtaagcaa
     1381 gggataagga taggcgtatt atgatgacga ccagaatagt ggttggcctc acggcaggga
     1441 cgtgtctgat tttctcgcaa aacctgatgg ccgaggtcag tgtattcaat ccggcgcttc
     1501 tggaaatcga ccatcaatcc ggagtcgata ttcgccagtt taatcgggca aacctgatgc
     1561 ccccaggtgt ttatagcgtt gatattttta tcaacggtaa aatgtttgaa cgtcaggatg
     1621 tgacatttgt tcaggataat ccagatgctg atctgcacgc ttgctttatt gccattaaaa
     1681 aaacactgtc ctcctttggc ataaaagttg atgcgctcaa atcgttcaat gatgtggatg
     1741 agacggtttg cctcgatcct gctccacgta ttgaaggctc atcctggcag tttgacagtg
     1801 ataaattgca gctgaatata tccattcccc aaatctacat ggacgcgatg gcttatgatt
     1861 acatcagccc cacgcgttgg gatgagggga ttaacgcgct caccatcaac tacgattttt
     1921 ctggttcaca tacactacgt tcagattatg gttcacaaga gacagatacc agttatctca
     1981 atctgcgcaa tggactgaat attggaccgt ggcggctacg taattacagt actttaaaca
     2041 ccagcgatgg ccgtgcggaa tacaactcca ttagtacctg gatacagcgc gatattgccg
     2101 cgttaagaag ccagattatg attggtgata cgtggacggc gagcgatatt ttcgacagta
     2161 cgcaaattcg cggcgcgcgt ttgtatactg ataacgatat gctacccgcc agccagaatg
     2221 gctttgctcc tgtggttcgt gggattgcaa agtccaacgc caccgtcatc attcggcaga
     2281 atggctacgt gatttatcag tctgccgttc cacaaggtgc ttttgagatc accgatctca
     2341 acaccgccag tacaggtggc gatttggacg taaccatcaa agaagaagac ggtagcgaac
     2401 aacgattcac ccaaccttat gcttcattgg cgattcttaa acgtgaaggt cagacagatg
     2461 ttgatgtcag cgtgggtgaa ttgcgcgatg aagacggatt tacaccggac gtccttcagg
     2521 cgcaaatact tcatggtttt tcccacggga tcactttata tggaggtatg caggctgctg
     2581 aaaattatgg ttctgcagct ctgggtgtcg gtaaagatct tggcgctttg ggcgcaattt
     2641 ctttcgatgt gacacatgct cgtgcgaatt ttagccatga tgatacagaa acgggtcagt
     2701 catatcgctt tctctattca aaacgatttg acgacacaga cactagcttg cgcctggttg
     2761 gctatcgtta ctccaccgag ggctactata ccctcaatga gtgggcatcg cggcgcaaca
     2821 gccctgaaga cttttgggaa acaggtaacc gacgtagtcg cgtggaggga acgctaacgc
     2881 agtcgttggg gagagattat ggcaatttat acctgacatt aagccggcaa caatactggc
     2941 ataccgatga tgtcgaacga ttaatgcaat ttggctacag cagtagctgg aagcgtctct
     3001 cgtggaacgt ctcctggagt tattccaata ctgccagaca ggggacgggg aacaaccatg
     3061 ccagtgataa caccagtgag cagatctaca tgctctcttt atctgttcct ttatcgggct
     3121 ggtggggtaa tagttacgcc acctattctg tttcgcaaaa cgataattcc ggtagctcac
     3181 atcaactcgg actcagcggt acggcgctgg aaagaaataa cctttcatgg aatttaatgc
     3241 agtcctataa cagtcatgat gatgaggttg gcggtaatat gtccctgacc tatgatggct
     3301 cttatggcac ggtgaacggc agctataact acagccaaaa ttcccagagg ctgaattatg
     3361 gtatcagagg gggaattctg gcacacagcg aaggggtaac gttaagtcag gagttaggtg
     3421 aaactattgc tcttgttaaa gcacctgggg ccgccgggtt agaaatagat aatatgcgcg
     3481 gtgctgcgac ggactggcgc ggctataccg tcaagacaca gctaaaccct tatgatgaaa
     3541 atcgggtagc aatcagcgat aactatttct cgaagtcgaa tatagaactt gataataccg
     3601 tcgttacgat ggttcccacg cgtggtgcag tggttaaagc ggagtttgtg actcatgtgg
     3661 gttatcgcgt tctcttcaga gtgttaaatg caaatggtaa accggtacct tttggagcca
     3721 ttgctgcgat acaagatgca agtttggcag attcaggaat tgtcggtgac cgtggcgaac
     3781 tttatctttc tggtctacca gaaaaaggac aggttacgtt atcctgggga gaaaacgcct
     3841 caacaaaatg catcttcaat tattcacttt cgacaccaga aagtgagagc ggattaattg
     3901 aacagggtgt gacatgtcat taaaaggatt tttatgaaca agtatatcaa acagtggtgc
     3961 tttgctgtgt ttatgctctc gttaagtagt gtagcacttg cagctcctaa aggtatctgt
     4021 acccctgata atggcgtatt tcacagtacg cttgatttct cgggatatct tattaccgcg
     4081 aacgaaaata aggttggaac gacatttaac accactgtaa ctaatggatc ctcatatcct
     4141 ggacgctgtc attgtgatac tggtaacgta ggagaattcc cctatatcta ttatacgtca
     4201 aaaataaacc aggcattaac ttatgctggg gttcactcta atattaatta ttatgatctt
     4261 aatccgaacc ttgatgttgg gattgctata gatattcttg gggttgggta tgtgaatgca
     4321 ccttttgaat atcatgctaa caacccgtct ggtaatacaa agtacaattg caaccgcatt
     4381 gaacctttaa gtatatctag tggggctaaa gcgatagtat atttttatat caagaagaca
     4441 tttgcaggaa aattgattat tcctgaaaca aagatagtga cattgtatgg aacaattagc
     4501 cgtgacaccc cggtggatta ctcacagccg atggctgatg tttatattcg gggcgatatt
     4561 acagctccgc aaagttgcga aataaataat ttacagccag tttattttga ttttaaagaa
     4621 ataccagctg cagatttttc atctgttgtt ggaagtgcgg taacaacaca taaaattact
     4681 aaaacggtca ctattgagtg tgaaaaccta gggatactga ataccgatga tatcagtacg
     4741 tctttttatg ctaccgaacc caatacggac aattcaatgg tcgtaacttc aaactctaac
     4801 gtggggataa aaatttacga caaaaataat aaggaaatca aagtgaacgg tggtgagttg
     4861 ccaacagaca tgggtaaatc aacagtgtat ggtgaaaaat caggtagtgt aactttttca
     4921 gctgctcctg cgagccttac tggagctcgc ccagccccgg ggcaatttac tgcaacagcg
     4981 acgataactg ttgagattgt gcgctaatag gtctgacaac gattgttcat cacatcgttg
     5041 tcatctcacc catttcttta tcacttttaa atgcatcatg acaaaaagat gacatgtggg
     5101 attttcatct tcacatttaa aatcagtaag ttatttggta attcctttct gtcataaaaa
     5161 tgtcatattc cttacatata actgtcacct gtttgtccta ttttgcttct cgtagccaac
     5221 aaacaatgct ttatgaatcc tcccaggaga cattatgaaa gttatgcgta ccaccgtcgc
     5281 aactgttgtc gccgcgacct tatcgatgag cgctttctct gtgtttgcag aagcaagcct
     5341 gacaggtgca ggtgcaacct tccctgcgcc ggtgtatgcc aaatgggctg acacttacca
     5401 gaaagaaacc ggtaataaag ttaactacca gggtatcggt tcttccggtg gcgtaaaaca
     5461 gattatcgct aataccgttg attttggtgc ctctgacgcg ccgctgtctg acgaaaaact
     5521 ggctcaggaa ggtctgttcc agttcccgac cgtgattggc ggcgtggtgc tggcggttaa
     5581 cattccaggg ctgaagtctg gcgaactggt gctggatggt aaaaccctcg gcgacatcta
     5641 cctgggcaaa atcaagaagt gggatgatga agccatcgcc aaactgaatc cgggtctgaa
     5701 actgccttca caaaacattg ctgtagtacg ccgcgcagat ggctccggga cttccttcgt
     5761 cttcaccagc tacctggcga aagtgaacga agagtggaaa aacaacgttg gtactggctc
     5821 taccgtaaaa tggccgatcg gtctgggcgg taaaggtaac gacggtatcg ccgcgttcgt
     5881 tcagcgtctg ccgggtgcaa ttggttatgt tgaatatgct tacgcgaagc agaacaacct
     5941 ggcgtacacc aaactgatct ccgctgatgg taaaccggtt agtccgaccg aagaaaactt
     6001 cgctaatgca gcaaaaggtg cagactggag caaaaccttc gctcaggatc tgaccaacca
     6061 gaaaggcgaa gatgcatggc ctattacctc taccacgttc attctgatcc acaaagatca
     6121 gaagaaacca gaacaaggca cagaagtgct gaaattcttc gactgggcgt acaaaaccgg
     6181 ggctaaacag gcgaacgacc tggattacgc cagcctgccg gatagtgtag ttgaacaggt
     6241 tcgcgctgcg tggaagacca atattaaaga cagtagcggt aagccgctgt actaataaaa
     6301 ctccaggccg ggtacggtgt tttacgccgc atccggcatt acaaaatgac tttgtaaacg
     6361 cgtttaactg aagagtaact tatggctgca accaagcctg cttttaaccc accgggtaaa
     6421 aagggcgaca taattttcag cgtgctggta aaactggcgg cgctgattgt gctattgatg
     6481 ttgggtggca ttattgtctc tctgatcatc tcctcctggc cgagcattca gaaatttggt
     6541 ctggctttcc tatggaccaa agagtgggat gcaccgaacg atatctacgg ggcgctggtg
     6601 ccgatctacg gtacgttggt gacttcgttt atcgcgctgc tgatcgccgt cccggtgagt
     6661 ttcggtatcg ccctgttcct gactgagctt gcgcctggct ggctgaaacg cccgctgggt
     6721 atcgccattg agctgctggc agccattcca agtatcgttt acggcatgtg gggcctgttt
     6781 atctttgcgc cgctgttcgc cgtttacttt caggagccgg tcggcaatat catgtcgaat
     6841 atcccgattg ttggcgcgct gttctctggc cccgcatttg gtatcggtat cctcgcggca
     6901 ggcgtgatcc tcgccatcat gattattccg tacattgcgg cggtaatgcg tgatgtgttc
     6961 gaacaaaccc cggtgatgat gaaagagtcg gcctacggta ttggctgcac cacctgggaa
     7021 gttatctggc gtatcgttct tccgttcacc aaaaatggtg ttatcggcgg catcatgctg
     7081 gggctgggcc gcgcgctcgg tgaaaccatg gcggtgacct ttatcatcgg taacacctac
     7141 cagctcgaca gcgcctcgct gtatatgccg ggcaacagta tcacctctgc gctggcgaac
     7201 gaatttgcgg aagcggaatc cggtctgcac gttgccgcac tgatggaact gggcctgatc
     7261 ctgtttgtga ttaccttcat cgtcctcgcc gcatcgaagt ttatgattat gcgcctggct
     7321 aagaatgagg gggcacgcta atggctatgg ttgaaatgca aaccactgcg gcgctggctg
     7381 aatctcgccg caaaatgcag gcgcgtcgcc gcctcaaaaa ccgtattgcg ctgacgctct
     7441 cgatggcgac gatggccttc ggcctgttct ggctgatctg gattttaatg tccaccatca
     7501 ctcgcggtat cgacggtatg tcgctggcgc tgttcactga aatgacgccg ccgcccaata
     7561 cggaaggtgg tggtctggcg aacgccctgg cgggtagcgg gctgttaatt ttgtgggcca
     7621 cggtattcgg tacgccgctg ggcattatgg cggggattta tctggcggaa tatggtcgta
     7681 aatcctggct ggcagaagtg attcgcttca ttaacgacat tctgctctcc gcgccgtcga
     7741 ttgtggttgg tctgtttgtc tacaccatcg tggtggcaca gatggagcac ttctccggct
     7801 gggcgggcgt aattgcgctg gcgctgttgc aggtgccgat cgttatccgc accaccgaga
     7861 acatgctgaa actggtgccg gacagcctgc gtgaagcggc ttatgcgctg ggtacaccga
     7921 agtggaagat gatctccgcg attacgctga aagcgtcggt gtccgggatt atgaccggta
     7981 tcctgctggc gattgcccgt attgccggtg aaaccgcgcc gctgctgttt accgcgctct
     8041 ccaaccagtt ctggagcacg gacatgatgc agccgatcgc caacctgccg gtgacgatct
     8101 ttaagtttgc gatgagcccg tttgcggaat ggcaacaact ggcctgggcc ggggtattga
     8161 tcattaccct gtgcgtcctg ctgctgaaca ttctggcgcg cgttgttttt gcgaagaata
     8221 aacacggtta acactttgcc ggatgcggcg tgaacgcctt acccggccta cggtaagcct
     8281 gattagcgaa aagcatcagg tacgatgagg aaaagattgc aatgagtatg gttgaaactg
     8341 ccccgagtaa aattcaggtt cgtaatttga acttctacta cggcaaattc catgccctga
     8401 aaaacatcaa cctggatatc gctaaaaacc aggtaacggc gtttatcggg ccgtccggct
     8461 gcggtaaatc gacgctgctg cgtaccttca acaaaatgtt tgaactgtat ccggagcagc
     8521 gcgcagaagg tgaaattctg cttgatggcg acaacatcct gaccaactct caggatatcg
     8581 ccctgctgcg tgcgaaagtg ggcatggtgt tccagaaacc gacgccgttt ccgatgtcca
     8641 tctacgacaa catcgctttt ggcgttcgtc tgtttgagaa gctctcccgt gccgacatgg
     8701 acgagcgcgt gcagtgggca ttgaccaaag ccgcattgtg gaacgaaacc aaagataaat
     8761 tgcaccagag cggttactct ctctctggtg gtcagcaaca gcgtctgtgt attgcgcgtg
     8821 gtatcgccat tcgcccggaa gtgctgctac tcgacgaacc gtgttcggcg ctcgacccta
     8881 tctctaccgg gcgtattgaa gagctgatca ccgaactgaa gcaggattac accgtggtga
     8941 tcgtcaccca caacatgcag caggctgcgc gttgttccga ccacacggcg tttatgtacc
     9001 tgggcgaatt gattgagttc agcaacacgg acgatctgtt caccaagcca gcgaagaaac
     9061 aaacagaaga ctacatcacc ggtcgttacg gttgattcag gagtgcgtta tggacagtct
     9121 caatcttaat aaacatattt ccggccagtt caacgccgaa ctggaaagta tccgcacgca
     9181 ggtgatgacc atgggcggca tggtggagca gcagctttct gatgcaatca ccgcgatgca
     9241 taaccaggac agcgatctgg cgaagcgcgt gatcgaaggc gacaagaacg tcaacatgat
     9301 ggaagtggcg atcgatgaag cctgcgtgcg cattatcgcc aaacgtcagc cgacggcgag
     9361 cgacctgcga ctggttatgg tgatcagtaa aaccattgcc gagctggagc gtattggcga
     9421 cgtggcggac aaaatctgcc gtactgcgct ggagaaattc tcccagcagc atcagccgtt
     9481 gctggtaagt ctggagtcgc tgggccgtca taccatccag atgctgcacg acgtgctgga
     9541 cgcgttcgcg cggatggaca ttgacgaagc ggtacgtatt tatcgtgaag ataaaaaagt
     9601 cgatcaggaa tacgaaggta ttgttcgtca actgatgacc tacatgatgg aagattcgcg
     9661 taccattccg agcgtactta ctgcgctgtt ctgcgcgcgt tctatcgaac gtattggcga
     9721 ccgctgccag aatatttgtg agtttatctt ctactacgtg aaggggcagg atttccgtca
     9781 tgtaggcggt gatgagctgg ataaactgct ggcgggaaaa ga
//
LOCUS       DAFNBJ010000174         9785 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000174 DAFNBJ010000000
VERSION     DAFNBJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.174"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(233..991)
                     /locus_tag="L2X49_004143"
     CDS             complement(233..991)
                     /locus_tag="L2X49_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBN4799144.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(999..2102)
                     /locus_tag="L2X49_004144"
     CDS             complement(999..2102)
                     /locus_tag="L2X49_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBN4799145.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2112..3293)
                     /locus_tag="L2X49_004145"
     CDS             complement(2112..3293)
                     /locus_tag="L2X49_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBN4799146.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3361..4386)
                     /locus_tag="L2X49_004146"
     CDS             complement(3361..4386)
                     /locus_tag="L2X49_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN4799147.1"
                     /translation="MKKMMIATLAAASVLLAFANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4817..5038)
                     /locus_tag="L2X49_004147"
     CDS             complement(4817..5038)
                     /locus_tag="L2X49_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799148.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5291..8395)
                     /gene="acrF"
                     /locus_tag="L2X49_004148"
     CDS             complement(5291..8395)
                     /gene="acrF"
                     /locus_tag="L2X49_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HBN4799149.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTVPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8407..9564)
                     /gene="acrE"
                     /locus_tag="L2X49_004149"
     CDS             complement(8407..9564)
                     /gene="acrE"
                     /locus_tag="L2X49_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HBN4799150.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2614 a         2538 c         2332 g         2301 t
ORIGIN      
        1 catcaaataa aacaaaaggc tcagtcggaa gactgggcct tttgttttat ttgatgtttg
       61 tcggtgaaca ctctcccaac caggacaaat ccgccgggaa cggatttgaa cgttgcgaag
      121 caacggcccg gaggctggcg ggcaggacgc ccgccataaa ctgccagaca tcaaatcaag
      181 caaaaaggcc atccgaaagg atggcctttt tgcttttcga actaacattc aattaatgga
      241 ttacctgcga taaaaatgcc ctcgtacgct ctgatttagg atgcgtaaaa aattcatcag
      301 gtgcagcttg ctccactatt tccccacgat ccataaaaat cacccggtca gcgacggttc
      361 gcgcaaaccc catctcatgc gttacacaca acattgtcat acccgattgc gccagcccaa
      421 tcatcgtatc cagcacctct ttcaccatct ccggatcgag cgccgacgtt ggctcatcaa
      481 acaacataat tttcggcttc atacacagcg aacgcgcaat ggcaacgcgt tgctgctgac
      541 caccagaaat ttgtccggga aacttatgcg catgttcggc aattctcacc cgctcaaggt
      601 aatgcatcgc cagagcttca gcctctttct ttggcatctt gcgtacccaa atcggtgcca
      661 gggtacagtt ctgtagaacg gtcagatgag ggaagagatt gaaatgctga aagaccattc
      721 ccacttcctg ccggacgcgc tcaatattgc ggatatcttc attaagttcg atgccatcta
      781 ccacgatccg tccctgttga tgttcttcca gatgattaat acaacgaatg gttgtcgatt
      841 taccggaacc tgaagggcca cacagaacga tccgttctcc cggttgcacg gttaaattta
      901 tatttttcaa aacatggaat tgtccatacc atttattgac gttttccagc gtaatcatcg
      961 cgttagcagg ttgcagtaaa atttggctca tagtgtcctc aatgcggtgt acgcccggtg
     1021 ttaaaacgtt tttccagatg ctggctatag cgcgacatgc tgaaacagaa gatccagtag
     1081 atcagcgcgg cgaaaacata cccttccgtc gacataccca accaggtggg atcaacggtt
     1141 gcctgctgaa cgctactgaa aagatcgaac aacccgataa tgatcaccag gctggtatct
     1201 ttgaagagtg cgatgatggt atttaccagc ccaggaatta ccagcttcaa cgcctgtggc
     1261 agaataacca gcccctgagt tttccagtaa cccaacgcca gcgactctgc cgcttcatac
     1321 tgccctttag gcagtgcctg taatccgcct cgcacgactt ccgcaacata tgctgactga
     1381 aacaggatca cgccaaccag cgcgcggatc agtttgtcga tactggtgcc ttctgccata
     1441 aacaacggca gcatgaccga agacataaac aatacggtaa tcagcggtac gccgcgccag
     1501 aactcgataa aaatgaccga taagatacgc acaatcggca tatgggagcg gcgacctaac
     1561 gccagtaaga tcccccacgg tagcgccccc gcaatcccaa cggatgcaat aattaaagtt
     1621 agcgtcagcc cgccccattg ccgggtttca acccgctcca gaccaaaaaa accgccatac
     1681 atcagccacc agacaatcag tgggtaaatc accgcccaga ccgcaatata gcgaccgcga
     1741 tgcgggagta ttttccagaa cattggtgcg attgacacaa ggccaatcag taatgccagg
     1801 ttaattcgcc agcgttggtc gtgtgggtaa agtccataca taaactgacc aaatcgttcg
     1861 tggatgaaga cccaacaggc accggctttt gtgcagtcgg cacgcgttga gccaacccaa
     1921 ttagcctgca aaaatgccca gttcagcaac ggtgggatca gttcccacat caaccatatg
     1981 cagccaatag tcagcaggct attgctccag ctggagaaca gattttttcg cacccacacc
     2041 atcgcgcggc ttgagttatg gctcgctggg cgcgagggct gagacagcaa tacttttgtc
     2101 atcatgactc cttagcgttc aacgatcgcg atgcggcggt tatagatatt catcagcagc
     2161 gagatagtca ggctgataat cagatagacc gacatggtca tggctatcgt ctcgatggct
     2221 tgccccgtct ggttcagcac ggtgccagca aacagcgaaa ccatatcggg atagccaata
     2281 gcggcggcaa gagaggagtt tttgacgatg ttgagatact ggctggttaa cggcggaata
     2341 atcactcgca gtgcctgggg aataatgacc tggcgtagcg taaccgggtt gggtaatccc
     2401 agtgaccgag ccgcttcatg ttgaccataa ggcactgcct ggatcccagc gcggataatc
     2461 tcggcgataa atgcagatgt ataaaccgaa agtgccagcg ttaaggctgc cagttcagga
     2521 attaaaacca tcccgccgcg gaagttaaag cctcgtaggg ctgggacatc ccagtgtagt
     2581 gctgcgccaa aaagccattg cgccagtaaa ggcaaaccaa tgatcaacac tgcggcgatc
     2641 ggccaggtgc ggcgcagttg tccggtcttt atctggtatg ttttattaaa gcggaatagc
     2701 ccaacagaaa ggactatagc cataacaaca gccaggataa acgcaataaa tccgtctccc
     2761 agctgcggtg acggaatata aagcccacga ttgctcaaaa aggccagatc aaacgcgctc
     2821 acagcctggc gtggtccggg caaattgcgc aacacggcaa agtaccagaa gaagatttgc
     2881 agcagcgggg gaatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     2941 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     3001 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     3061 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     3121 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt gtgaaataac
     3181 caaccaacaa tgccgacaac agcaacaacg gcaaggatct ggaataacca ggcacgaacc
     3241 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3301 tcggaaagca taacgctgaa gagagcgccg ccgtcgcgac gctcgttacc tcacaccgac
     3361 ttaacgcacg ggcggtgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3421 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3481 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3541 gccttcttta cccagcagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3601 aacgttctgg gaattgatgc ccatctcttc agcattcagc atggcgaaaa gcgtccagcg
     3661 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3721 gataacctcc ggtaagacaa tccattcagc tgggttgctt aatttgatgc gcagggcata
     3781 cagttgcgac tgatccgagg ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3841 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3901 ggcaacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3961 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     4021 gacgccagta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     4081 ggagagcaaa tccacttccc ctgactgtaa agccgtgaag cgttcttttg ctgtgagtgg
     4141 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4201 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacccag gtaatccatc
     4261 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4321 cgcctgattt gcaaaggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4381 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4441 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcccgatttc
     4501 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg
     4561 aaaatgaaac atgcgccaca ttttggcgca aaatatttac gcgtgtttta ctaagcagaa
     4621 taatgattgt tagcgtgagt atatttctgg tgggaagtgt gaaagttaaa ttgatgcgtt
     4681 atttcgaaag cagcgaaata acgcgatata acatgctaaa tcagcagaga attagccagc
     4741 agaatatagc aatattttat cttatgtaaa gatactgtat cgttaaacag agcgcgaaac
     4801 tccgcgccct gttcagttat ttcgacagag cgatgatccc catcgtcagg ccagcaacag
     4861 ccgctgcccc gccagcaata gccccagccg tttcccagtt atcctgagtc gtgcctttca
     4921 tacaggtatt ggtgtgaacc agacgaccct gatcgtcata aaccggaacg cagggcgaat
     4981 cgtgagcaca gcccgccagc aatgcggtga gcaatgccac aggaattaat cttttcatgg
     5041 tgttacctca gtaataaacg ctaacgctta ataagttaac tctcttatta acgagctgaa
     5101 tgccatttta tcaccggagg ttataaatct tgcggaatga ataatgacaa attatgtgaa
     5161 ttgtaaaaat agtgaaggga gcgggaacta tagaaagctt ttaaagagtg agcagaagaa
     5221 tatcttatta atttttatgg gtttcactgg aaataataaa ggcacccgaa agcgccttta
     5281 tgtttctgat ttatccttta aagcaacggc ggatcaccac aaagaacacc ggtacgaaga
     5341 agattgccag caacgttgca gagaccattc ctcccattac cccgataccc acagcgttct
     5401 gcgcgccact gccggcaccg ttactgatag ctagcggtaa tacgccgaga ataaaggcga
     5461 gagaggtcat caggatagga cgcagacgca tacgtactgc cattagtgtc gcttcaacaa
     5521 cacctttacc ctctttctcc atgagatctt tagcgaactc aacaatcaaa atagcgtttt
     5581 tggccgacaa gccaattgtc gttagcaagc ccaccataaa gtagacgtca tttttttgat
     5641 taaagagtgt cgccgccagc agcacgccga caatccctaa cggcacgacc aacataaccg
     5701 agacaggaat tgaccagctt tcatagagtg cagcaaggca caggaaaaca accacaaagg
     5761 aaattgctac cagagcggga gcctggtttc ccgataagcg ttcctgatac gacatacccg
     5821 tccagtcata accaatgcca gcaggtaatt ttgacgcaag gttttccatc aacgccatgg
     5881 catcgccgga actggttcct ggcgcggctt ccccctgaat ctccattgac ggcagaccgt
     5941 tgtagcgttc cagtcgcgga gagccataca cccaatgtga agtggtaaag gccgagaatg
     6001 gcaccatttc gccgttggcg ctgcggacat aaagtttatc gacatcttct ggcagcatac
     6061 ggaatttggc atccgcctga acatacacct ttttcacgcg accacggtcg atgaagtcgt
     6121 taacgtaagt cccacccaac gccgttgaaa tggtctgatt gatgtcagaa agtgaaacac
     6181 ctaatgcctg cgccttttcc tggtcaactt ccagtttaaa ctgcgcggtg tcttccaggc
     6241 cattaggacg cacgctgact aagctggcag gatgttgcgc cgccatacca agcaactggt
     6301 tacgggcctg ggttagggca tcgtgaccca gcccagcctg atcaattaac tcaaagtcga
     6361 aacccgttgc cgtgcccagt tcaacaatgg ctggcatatt gaatggaatg acaaaaccgt
     6421 cgcggatctt gccaaattcc attttggcac gatggattac cgcttccgca ctgttttcat
     6481 caccattacg ctcttcccac ggtttcagac tgacgaaggc cataccggcg ttttgtgcct
     6541 ggccgctgaa gctaaagccg ttaaccgtaa agacactttc aacgttcgct ttctcgttct
     6601 tcagatagta atccgtcact tgatccaaca ctttttgcgt ccgctcttgc gtcgcgccag
     6661 cgggtaactg aatcatggtc agaaagacac cctgatcctc ttcaggtaag aaggaagacg
     6721 gaagacgtaa aaacaacacc accattcctg caacaatcag cgcatagatc agtaaatatc
     6781 gtcctgtgga accgaggatt ttgccgacgc tgttggtgta gtggttaacg ctatgatcga
     6841 aggtagtatt aaaccagccg aagaaaccgc ccttattttc gtgatgctca gcagagacgg
     6901 gtttaagcag cgttgcacat aacgcagggg taagaatcaa tgccaccaga acagaaagcg
     6961 ccattgccga aacgatggtg atagagaact ggcgataaat tgccccagta gaaccgccga
     7021 agaatgccat cggaataaat accgctgaca gcaccatcgc gatacccacc agtgcgccct
     7081 ggatttgcga cattgatttt tccgtcgctt cttttggcgg gagcttatcc tccatcatca
     7141 cgcgctcgac gttctccacc accactatcg catcatcgac gagcagcccg atggcaagca
     7201 ccatcccgaa catcgttagt gtgttgatgg agtaaccaaa agcggcgagg atggcaaacg
     7261 tccctaacaa caccacgggt accgcaatgg tggggatcag cgttgctcgc atattctgca
     7321 agaacagata catcaccagg aacaccagca taatggcttc gaacagcgtt tttaccactt
     7381 cgtgaataga aagctggacg aatggcgtgg tgtcataagg gtagagaacc ttcattccct
     7441 gcgggaagaa tggctgtaat tccgccagtt ttgccttaat ggctttcgcg gtatcgagag
     7501 cattcgcgcc ggttgccagc ttaatcccca ggcccgccgc cggttttccg ttgatacgag
     7561 cgataacgtt atagttttca ccgccaagtt caacccgtgc gacatctttc aggcgtacca
     7621 ccgagccgtc actgtttacg cgcagggtca ctttgccgaa ttcttccgga ttttttaacc
     7681 gcgtttgagc aataatcgag gcattcaact gttgccctgg taacgctggc gttccgccca
     7741 actgtccggc agcgatctga tcgttctgta ccttcaactg gttaatcaca tcaaccggtg
     7801 tcagtttata tttgtttagc agatcggcgt ccagccagat acgcatcgca tactgtgcgc
     7861 cgaaaagctg tacgtcaccg acgccattca gacggctaag cgtatcttta acgttagagg
     7921 ccacatagtc cgagatatcg tcctgtgtgg tgtctgggtt atcagagaca aagcccgcca
     7981 ccatcaaata gctgctactg gacttttcaa cactgatccc ctgctgctga acctcctgcg
     8041 gcagcaacgg cgtggcgagc tggagtttgt tctgcacctg cacttgcgcg atatcaggaa
     8101 cggtcccgga ctggaaggta agggtaattg tcacgctacc ggcggaatcg ctggtggagg
     8161 acatatacat caggttatcg ataccattca tattctgttc gataacctgc gtcaccgtat
     8221 cctgcacggt ctgcgcatca gcacctggat agtttgccga aacagaaacc gcaggcggtg
     8281 caatggttgg atactgagcg acgggcaatt gtaggatcgc cagtgcgccc gccatcatca
     8341 gaataatggc cagcacccat gcaaatatcg gtcgtcgaat aaaaaagttt gccatgtcag
     8401 attaccttac ttcgatgcag tatctgcggg ggtatcggta gtggctttaa cctgctctcc
     8461 cggacgcgct ttttgcaggc cgctgacaat gacttgatcg ccagatttca gaccttcgct
     8521 aatcaaccat ttatcgccaa tcgcctgact ggcaacgaca gggcgcgctt ccacctgact
     8581 tttatcgtta acaatcagca cggttgcatc accacgcggt gtgcggctaa cgccttgttg
     8641 cgggataaga atggcgtcag gttggacgcc ttcatcaatc cgtgcacgca caaacatacc
     8701 cggcaaaagc gtatgttgcg ggttagggaa gacagcacgt agggttatgg agccggtgct
     8761 ttcatcaacg gtcacatcag agaattgcag cgtacctttc aggggatagg tttgaccgtt
     8821 ttccatgacc aactctacgt tgctggtggc gttttcctta tgcaaatttc cttgctctac
     8881 ggattgcttc agcctcataa aatcgttgct ggattgggtc acatcaacgt agataggatc
     8941 gagctgctgg acagtcgcca gttcagtcgt ttgcccatta gtgacaagag cgccttcggt
     9001 cacggtcgat ttgccgatac gtccgctaat tggcgcagtc actttggtat aagcgagatt
     9061 gatgcgagcg ctttcgactg tggcttttgc ggcaatcacg gcggcatcgg cctggcgagc
     9121 atcagcaatg gcctggtcgt actcctgctg gctgatgtat ttcgtaccca cgagcggaac
     9181 gtaacgtttt accgtcaaat gcgcgatggc ggcggcggct tcacttttcg ccagttcgcc
     9241 tttcgcgctg tcataatttg cctgataggt cgcgggatcg atctggtaca gggactggcc
     9301 tgcttgtaca tcgctgcctt cagtgaaatt acgattcagt acgatcccgc taacctgtgg
     9361 gcgaacttcg gctatacggt aagcattggt gcggcctggt aattcagtct taacttctaa
     9421 cggggccgtt ttcacaatat gaacggtaac ctgcggttca ccgacgtgag ctttctcttc
     9481 tcccttatcg ttacaaccgg cgattaaagc cgcggagatc agaataaagg agggcaggag
     9541 gaaaaacctg gcatgtttcg tcattactat tcctcaaaaa accaaaagcg cgttatttac
     9601 ccagaaaggc aacgcgttaa ctcgcagaaa gaaaaataca gtttgctatc ctacaaatta
     9661 tcattcgtcg atgtaaggaa tagttatgaa tactggcgtc tcaaggcaca taaacacaaa
     9721 aaaagattaa tattctactg ttttattttg acgcgggttg aaagaggcag aattaaaacc
     9781 tcgta
//
LOCUS       DAFNBJ010000175         9546 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000175 DAFNBJ010000000
VERSION     DAFNBJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.175"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(236..2227)
                     /gene="tkt"
                     /locus_tag="L2X49_004150"
     CDS             complement(236..2227)
                     /gene="tkt"
                     /locus_tag="L2X49_004150"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBN4799151.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2505..3263
                     /gene="loiP"
                     /locus_tag="L2X49_004151"
                     /gene_synonym="yggG"
     CDS             2505..3263
                     /gene="loiP"
                     /locus_tag="L2X49_004151"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBN4799152.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKALSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3469..4389)
                     /gene="speB"
                     /locus_tag="L2X49_004152"
     CDS             complement(3469..4389)
                     /gene="speB"
                     /locus_tag="L2X49_004152"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBN4799153.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4525..5256)
                     /locus_tag="L2X49_004153"
     CDS             complement(4525..5256)
                     /locus_tag="L2X49_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799154.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGMDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(5402..7378)
                     /gene="speA"
                     /locus_tag="L2X49_004154"
     CDS             complement(5402..7378)
                     /gene="speA"
                     /locus_tag="L2X49_004154"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBN4799155.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7387..7518)
                     /gene="yqgB"
                     /locus_tag="L2X49_004155"
     CDS             complement(7387..7518)
                     /gene="yqgB"
                     /locus_tag="L2X49_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBN4799156.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            7654..7869
                     /locus_tag="L2X49_004156"
     CDS             7654..7869
                     /locus_tag="L2X49_004156"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799157.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8173..9327
                     /gene="metK"
                     /locus_tag="L2X49_004157"
     CDS             8173..9327
                     /gene="metK"
                     /locus_tag="L2X49_004157"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBN4799158.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2360 a         2535 c         2424 g         2227 t
ORIGIN      
        1 agcagggaaa ccgatgtttt gaaaacagtg aattattaat ttcttatata acattgagtt
       61 atagatataa caacaaatgt caatacgcat atcgtgatgc atattacata attgatattt
      121 attgcatata aatatttgtg atctacaaca cgctttatct attgcttgtc cgcaaacgga
      181 catatcaagg taataaaaaa ggtcgccgaa gcgacctttt tacccgaaat gctaattaca
      241 gcagttcttt tgctttcgca acaacgttat caacagtgaa gccgaactct tcaaacagca
      301 gctctgccgg agcagattca ccgaaggtgg tcataccgac gatagcaccg ttcaggccaa
      361 catacttgta ccagtagtca gcaatacccg cttctacagc aacgcgtgca gtaaccgctt
      421 tcggcagtac ggattcacgg taagcagcat cctgcttgtc aaatgcgtcg gtagacggca
      481 tggacaccac gcgcgctttc acgccttcgg cagtcagttt ttcgtaggca gcaacagcca
      541 gttcaacttc tgaaccggta gcgatgaaaa tcagttccgg ctgaccggcg cagtctttca
      601 gcacataacc accgcgcgcg atgtttgcca gttgctcttc agttcgttcc tgctgcgcca
      661 ggttctgacg ggagaggatc agtgcggtcg ggccgtcctg acgctcaaca ccgtatttcc
      721 acgcgaccgc ggattcaacc tggtcacacg gacgccatgt agacatgttc ggggttacgc
      781 gcagagaagc gacctgctca accggctggt gagtcgggcc gtcttcgccc agaccgatgg
      841 agtcgtgggt gtaaaccatc acctgacgct gtttcatcag cgcagccata cgtacggcgt
      901 tacgtgcgta ttccacgaac atcaggaagg tggaggtgta cggcaggaaa ccaccgtgca
      961 gggagatacc gttagcaatc gcggtcatac cgaactcgcg aacaccgtag tggatgtagt
     1021 tacccgcagc atcttcgttg attgctttag aaccagacca cagggtcagg ttagacggtg
     1081 ccaggtcagc agaaccgccg aggaattccg gcaacagcgg accgaaagct tcgatagcat
     1141 tctgagacgc tttacggctg gcgattttcg ccgggttagc ctgcagttta gcaatgaact
     1201 cttttgcttt cgcgtcgaag tcagacggca tttcgccttt catacggcgg gtaaattcag
     1261 cggcttcctg cggataagct ttcgcgtaag cagcgaattt ctcgttccat gcggattctt
     1321 tcgcctggcc tgcttctttc gcatcccact gagcatagat ttcagacggg atttcgaacg
     1381 gtgcatattt ccagcccagc tgttcgcggg tcagggcaat ttcagcgtcg cccagcggcg
     1441 caccgtggga gtcgtgagta ccggctttgt tcggggaacc gaaaccgatg atggttttgc
     1501 acatcagcag ggacggtttg tcagtcactg cgcgcgcttc ttctactgcg cgtttgatgg
     1561 atgccgcgtc atgaccgtcg atgtcgcgaa taacgtgcca gccgtaagct tcgaaacgca
     1621 ttgcggtgtc gtcggtgaac cagccttcaa cgtgaccatc aatagagata ccgttgtcat
     1681 cgtagaacgc aatcagttta cccagcttca gcgtacccgc cagagagcaa acttcgtggg
     1741 agatgccttc catcatgcag ccgtcgccca tgaaggcgta ggtgtagtgg tcgacaatgt
     1801 cgtggcccgg acggttaaac tgcgccgcca gcgttttttc tgcaatcgcc atacctactg
     1861 cgttggcaat accctgcccc agcggaccgg tggtggtttc cacaccagcg gtgtaaccca
     1921 cttccgggtg gcccggagtt ttagagtgca gctgacggaa gtttttcagt tcttccatcg
     1981 gcagatcgta accggtgagg tgcagcaggc tgtagatcag catggagccg tggccgttgg
     2041 acagcacgaa gcggtcacgg tcagcccagg acggattctg cgggttgtgt ttcaggaaat
     2101 cacgccacag gacttcggca atgtcagcca tacccatagg ggcacccggg tgaccggatt
     2161 tggctttctg tactgcgtcc atgctcagcg cacgaatagc attggcaagc tctttacgtg
     2221 aggacatttt gactccagat cggatgatga agggcacgcc cttaacgact tgacgacagc
     2281 gcgttttggg ctacgccgga aaatttgcca acaatttacc gcaagccgcg cgtcatgtac
     2341 atggaacatc cttttgccgc ttcagaaatc tctggatcat gctcgcatgt tgcgcaatct
     2401 actcgcccgt ccgctgcgct tttccttata ctgagactga gcgtcgattc acctgcaaac
     2461 ggcgcatttt tagaataatc ctgaccttgt gcggaagaga aaacatgaaa attcgcgcct
     2521 tattggtagc aatgagcgtg gcaacggtac tgaccggttg ccagaatatg gactccaacg
     2581 gactgctttc atcaggagcg gaagcttttc aggcttacag tttgagtgat gcgcaggtga
     2641 aagccctgag cgatcaggca tgtcaggaga tggacagcaa ggcgacgatt gcgccagcca
     2701 atagcgaata cgctaaacgt ctgacaacta ttgccaatgc gctaggcaac aatatcaacg
     2761 gtcagccggt gaattacaaa gtgtatatgg cgaaggatgt gaacgccttt gcaatggcta
     2821 acggctgtat ccgcgtctat agcgggctga tggatatgat gacggataac gaagtcgaag
     2881 cggtgatcgg tcacgagatg gggcacgtgg cgttaggcca tgtgaagaaa gggatgcagg
     2941 tggcgcttgg tacaaatgcc gtgcgagtag ctgcggcctc tgcgggcggg attgtcggca
     3001 gtttatctca atcacaactt ggtgatctgg gcgagaaatt agtcaattcg caattctccc
     3061 agcgccagga agcagaagcc gatgattatt cttacgatct tctacgccaa cgcggcatca
     3121 gcccggcagg tcttgccacc agctttgaaa aactggcgaa actggaagaa ggccgccaaa
     3181 gctcaatgtt tgacgaccat ccggcatccg ccgaacgcgc ccagcatatt cgcgatcgta
     3241 tgagcgcgga tggggttaag taaagcctgg tggtgtcgaa cgtaggtcag ataaggcgtt
     3301 cacgccgcat ctgacatgaa tggcacattt gttaccttgt gcgcattgcc ggatgcgatg
     3361 ctggcgcatc ttatccggcc tacgggtgcc gaacgtaggt cggataaggc gttcacgccg
     3421 catccgacat taatggcacg ttttacccgt gcgcatcgca tctggtgctt actcgccctt
     3481 tttcgccgcc tgaatataca gcatctccag cgccagcgtc gccgcagcca gagcggtgat
     3541 ttccgactga tcatatgccg gagccacttc cactacgtcc atcccaacga tgttgagatc
     3601 tttcaggccg cgtaccagtt taatagcgcg atcggaggtc aggccgccaa tcactggcgt
     3661 accggtgcct ggtgcaaaag caggatccag gcagtcgata tcaaaggtca ggtaaaccgg
     3721 catatcaccc acaatctgtt tcacctgggc gataacgtca tccacgctac gatcgttcac
     3781 ctggcaggcg tccagtacgg taaagccgtt gtctttatca aactcggtac gaataccaat
     3841 ctgcacggaa tgattcgggt cgatcagacc ttctttcggc gcggtataga acatggtgcc
     3901 gtggtcaaat tcacaaccgt tcgcgtaggt atcggtgtgg gcgtcgaagt gtaccagcgc
     3961 cattttgccg aaatgcttag catgagcacg cagcagcggc agcgtaacaa agtggtcacc
     4021 accgaaagag agcatacgct taccggcagc cagcaacttc tcggcgtgtg cctgcagttt
     4081 ttcgctcatc tcacgggcat cgccgaaggc atataccaga tcaccgcagt ccacgacgtt
     4141 caggcgctca cgcatgtcga aattccacgg gaagcggttg tgttcccagg ccagattcgt
     4201 cgaaacctga cggatcgctg ccggaccgtg acgaccaccg gcacggccag aggtggccat
     4261 gtcgaacggc acgccggtga tcacccagtc ggcatcgctg tcatacggct ggaagttcat
     4321 cggcaggcgt aaaaaaccaa aggcgttgga aaccagtgag ttatcgtatt gatgacctaa
     4381 ggtgctcatg gatggactcc tattacaaag tcgatataaa aaaacccttc cgcgtcgtta
     4441 ggcccgacga ggaagggttg gatttgcgaa acacaagttt gtgctaatta tcgccttaaa
     4501 tgggcgaggc atcaagcgag gttgctattt ccgacacggt ggcggcgcat agccattgtt
     4561 atctgccgga aaggcttgtc cagagcacgg cggcagcgcg ttttgcgccg ggccttgtaa
     4621 ttcaaaggcg gcaaagccga ttaccccggt attacgcaca tcgccttgcg ccgaattggc
     4681 tgcgtaggaa tcagcaacgt tggcgaactg aaaagccgct tcggtatgtt tatctttgcg
     4741 gaatccttta atcgctaacg aatctccggc attcacaata tagccgttgt tattcagcga
     4801 accttgcttg ccgttcagca catccatgcc atcaaccgta gcaacgatct cgtagttggt
     4861 gttacgactg taattacgga catataattg ataattcatt ccgactctgg catggacaat
     4921 atagcgtggt tcattattgg atgaatcata aacccgcgta ataggtagag aattaaaatt
     4981 accatcgcga atagcatatt ccagttcgtt aatacgaatg ctataaacgc ggtcgtagcc
     5041 ggaaggaaca ttagtcgaat aactaataac gatcatctct gccggatcgc gccaggctcg
     5101 ctcagcagat acgccctgaa ccgttgagtg cacatcccca ccccaggcag tgccgacatt
     5161 gcccgatgca ctgctattat actgcgcatt actggaacaa ccggccagca gcacgattaa
     5221 tgccaccaac gcgctacgta ctttccattt tttcataacc gttccctctg catttaatta
     5281 atgcagcgat tgtagggaga ttatttaata agaaaagcag cgagattatt tttaagaata
     5341 tataaaaaga aagcgggccg atttacgtaa ataaatcgac ccgccagcgg aataatctta
     5401 attattcatc ttcaagatag gtataaccgt acaaacctgc ctcgaactct tcaaggaact
     5461 gttgttgcag ttcagcatca agatcggttt tcttcacttg atcgcggaac tgggttaaca
     5521 gcgttttcgg atcgagttgt acatattgca gcatgtccgc cacggtatcg ccttcgtcag
     5581 acagttctac ttctacgcta ccgtcaggga acacgaacac gtcaaccgct tcggtatcac
     5641 cgaacaggtt gtgcatattg ccgaggatct cctgatatgc gccgaccata aagaaaccga
     5701 gcatcggcgg attctctgga tcgtactccg gcattggcat tgtcgtggca ataccatcac
     5761 catcaatata gtggtcgata gcaccgtcag agtcacaggt aatatccagc agcacagcgc
     5821 gacgttccgg cacttgatcc aacccttcca gcggcagaac cgggaacaac tggtcgatcc
     5881 cccacgcatc cggcatcgac tggaacagcg agaagttgac gtacattttg tccgccatac
     5941 gttcctgcag ctcgtcgata atcggacgat gagcacggtt ttgcggatcc agttgcttct
     6001 gcacttcatg gcacatgctc agatagagtt gttctgccca tgcacgttct tgcaggctaa
     6061 aggtgccgga agagtagccg atatgaatgt cgtgcagatc catctgactg tcgtgtaacc
     6121 attcacgcag agaacggcga gttcccggtt cgtgcatctc ctgccaggtt tcccacatgc
     6181 tttgcagcgc gcgcggcgca tcttctgcag gcgcggtcgg caccgtgtat tcgttacgtt
     6241 ccacgccgat gatattagat accagcacgg tgtgatgcgc agtcaccgca cgacccgatt
     6301 cggtgattac cgtcggatgc ggcagaccgt tttcttcaca cgcatcacca atcgcccaga
     6361 tgatgttgtt ggcatattca ttgaggccgt agttcaccga acagtcggac tgcgaacggg
     6421 tgccttcata gtccacgccc agaccgccgc cgacgtcgaa gcactgaata ttgacgccca
     6481 gcttgtgcag ttccacatag aaacgcgcgg attcacgaac gcctgtcgcg atatcgcgaa
     6541 tattcgccat ctgcgaaccg aggtggaagt gcagcagttg caggctgtcg agacgcccgg
     6601 cttcacgcag ggtttcaaca agttgcagta cctgagtcgc agccaggccg aacttcgatt
     6661 tttccccgcc ggaggactgc catttacccg agccctgcga agccagacgt gcacgcacgc
     6721 ccagacgagg aacgacattc agacgttctg cttcatccag cacaatggcg atttctgaca
     6781 tcttctcaat gaccagatag accttgtgcc ccatcttctc gccaattaat gccaggcgga
     6841 tatattcgcg gtctttataa ccgttgcaga cgatgacgct acgggtcatg ccagcatgcg
     6901 ccagcaccgc catcaactct gctttcgagc ctgcttccag ccccaacggt tcgccagaat
     6961 gaatcaggga ctcaatcacg cggcggtgct ggttaacttt gatcggataa acaaggaagt
     7021 aatcgccgtt atagccgtag gattcccgcg cacgtttgaa cgcggcgtta atggaacgca
     7081 aacggtgctg caggatctgt gggaaacaga acagtgcagg cagacgctga ccctgtgctt
     7141 cacgagtttt cactaactgc gcgagatcga cgcgagcttc cgggacgtcc gggtccgggc
     7201 acacgctgat gtggcccagc tcgttaacgt catagtagtt attgccccac caggcaatat
     7261 tgtaagtacg cagcatcttg ctggcttcct gggagctcat tgcaacctcc tgcatggagc
     7321 gtagtacacc gtgttcgccc gctgacgaag gcaaacccat agacatgtcg tcagacatag
     7381 cgaacctcaa attattttat taagtgtaaa acagttaacg actatcgcag cctggaactg
     7441 cgataacaac ccataagcac atggattttc cagcagtgaa tgctgacgct ccaactgcgc
     7501 aaccggtttc tttttcataa cattattaag cacataaccg aacgtaagtg tgacagttcg
     7561 gcgaaaccac gagaaaactc ttgtttttac aagagcgccc ttgttcagtc ctcagtaact
     7621 gtaaccagct cttgaatcct gagaagcgcc gagatgggta taacatcggc aggtatgcaa
     7681 agcagagatg cagagtgcgg ggaacgaatc ttcaccagaa cggtgagaca ggttaagcag
     7741 cagacaacgg ttcattattt cgtatcacct ccacggccgc ctgttaagac gaacccacaa
     7801 gccaaaactc tgatttcaac ccggctggaa gtggcaacac gaaagaaacg tcgtgtgctt
     7861 tttatttaag ccgcgcgccg cgttttatac cccacaatgg cagaaaattg caaaagataa
     7921 atacgcagaa tgccggcatt gtcaggaaaa atttccagcc acgtttttaa cagaatgaga
     7981 cacgattcaa aaaaaagtgg aaatcgggtg aagaattgac ctaaaatagc catccagatg
     8041 ttaatccatc cataccgatt aacactcaga ctgccagtgt ttttaacttg cagagtcgtg
     8101 gtaggatccg ctaccacaga aaatccacac aacagtttga gctaaccaaa ttctctttag
     8161 gtgatattaa atatggcaaa acaccttttt acgtccgagt ccgtctctga agggcatcct
     8221 gacaaaattg ctgaccaaat ctctgatgcc gttttagacg cgatcctcga acaggatccg
     8281 aaagcacgcg ttgcttgcga aacctacgta aaaaccggca tggttttagt tggcggcgaa
     8341 atcaccacca gcgcctgggt agacatcgaa gagatcaccc gtaacaccgt tcgcgaaatt
     8401 ggctatgtgc attccgacat gggctttgac gctaactcct gtgcggttct gagcgctatc
     8461 ggcaaacagt ctcctgacat caaccagggc gttgaccgtg ccgatccgct ggaacagggc
     8521 gcgggtgacc agggtctgat gtttggctac gcaactaatg aaaccgacgt gctgatgcca
     8581 gcacctatca cctatgcaca ccgtctggta cagcgtcagg ctgaagtgcg taaaaacggc
     8641 actctgccgt ggctgcgccc ggacgcgaaa agccaggtga ctttccagta tgacgacggc
     8701 aaaatcgttg gtatcgatgc tgtcgtgctt tccactcagc actctgaaga gatcgaccag
     8761 aaatcgctgc aagaagcggt aatggaagag atcatcaagc cgattctgcc cgctgaatgg
     8821 ctgacttctg ccaccaaatt cttcatcaac ccgaccggtc gttttgttat cggtggcccg
     8881 atgggtgact gcggtctgac tggtcgtaaa attatcgttg atacctacgg cggcatggca
     8941 cgtcacggtg gcggagcatt ctctggtaaa gatccatcaa aagtggaccg ttccgctgcg
     9001 tacgcagcac gttatgttgc aaaaaacatc gttgctgctg gcctggccga tcgttgtgaa
     9061 attcaggttt cctacgcaat cggcgtggct gaaccgacct ccatcatggt agaaactttc
     9121 ggtaccgaga aagtgccttc tgaacaactg actctgctgg tacgtgagtt cttcgacctg
     9181 cgcccatacg gtctgattca gatgctggat ctgctgcacc cgatctacaa agaaaccgca
     9241 gcatacggtc actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa agcgcagctg
     9301 ctgcgcgatg ctgccggtct gaagtaatct ttcttcacct gctttcaaag gccagcctcg
     9361 cgctggcctt tttcttttac ctcaccgcgg tggataggca ttcacgccgc atccggcaaa
     9421 aagctgcccg cacaataaca tcattcttcc tcaacacgtt ttatcgcaaa ttatcatcac
     9481 tactgaaacc gattacacca ataacaccag ccaaatattt gtaatatttt catattatta
     9541 ttcagt
//
LOCUS       DAFNBJ010000176         9540 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000176 DAFNBJ010000000
VERSION     DAFNBJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.176"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            284..1210
                     /locus_tag="L2X49_004158"
     CDS             284..1210
                     /locus_tag="L2X49_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799159.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLSVYFQSPVILKKIQTFNGKIKFRQFEGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYVCPVQQIIDTPLIGYEYVLIQRILARELKFPSPIVGARKTNHGIIVRFSEELFQ
                     IFETMSWKERVEKQISRLPKNTALDVIKKLTEVTTIKYNHNGCFPLYTLPPDACFVIR
                     HTEVERLINLYKKRESHPISPSRMTTPLSRLFWLACKHNDIISPLLNHPYKLLSIFEQ
                     WASGDGIGEKLDAETLKNALKRGSPSSTSLSG"
     gene            1350..1535
                     /locus_tag="L2X49_004159"
     CDS             1350..1535
                     /locus_tag="L2X49_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBN4799160.1"
                     /translation="MTSYQLLRLRQVEQKTGLKRSQIYLYMKEGAFPRSIKIGPASVA
                     WLESEIDEWINKKLSDR"
     gene            1557..1739
                     /locus_tag="L2X49_004160"
     CDS             1557..1739
                     /locus_tag="L2X49_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799161.1"
                     /translation="MNINIPALINFDPAGIKKSTCMYISDLPGIVLYDTIPFTEYSAE
                     LLSTYSVKESISCFHP"
     gene            1724..2305
                     /locus_tag="L2X49_004161"
     CDS             1724..2305
                     /locus_tag="L2X49_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HBN4799162.1"
                     /translation="MLPSLNHIILTVALQALREGNIHHCETMGFTYDEMNLLGCLSIN
                     DLITLSQAPLPLVDITIRHDVLQKLLASSHEENRRQEQLNRAVRLGGSIALMNRYFGV
                     GSRETCARRRLLGVSVPNGRTPIPDEETDAAIWHQWQKYRVENIDSPEALDAMMLVTE
                     NLSSCTAPSLTVVWNCITHCERKNTVRRIQRAR"
     gene            2295..2504
                     /locus_tag="L2X49_004162"
     CDS             2295..2504
                     /locus_tag="L2X49_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBN4799163.1"
                     /translation="MPDEIDRDQAFNERCLEALIEQSRLRPTPTPSLQHCRLCGKAIP
                     EKRRQTLPGVTTCTDCQSILEKRRR"
     gene            2708..2875
                     /locus_tag="L2X49_004163"
     CDS             2708..2875
                     /locus_tag="L2X49_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799164.1"
                     /translation="MNDGSLLEKVVGHMEEKLTGNELEMLYAAADYRRAEIVMNQYFD
                     KVPGNAWKYVR"
     gene            3444..3677
                     /locus_tag="L2X49_004164"
     CDS             3444..3677
                     /locus_tag="L2X49_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799165.1"
                     /translation="MRKPVYYAYVAQESHPDSQGEKNTWWTKVGVAFPHHGKSGLNIV
                     LTPGIAVSGKLVLLEPREENNPPQNTGPIETVS"
     gene            3750..4460
                     /locus_tag="L2X49_004165"
     CDS             3750..4460
                     /locus_tag="L2X49_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBN4799166.1"
                     /translation="MLSTTAFLAIAMQCAASIHPSTAFDVARVESGFNPYAIAEIVPK
                     AGPSFGDQHIISHLPASRGDAEHILRRLSAQDRRYSVGLMQITSTNFRHYGVSATELL
                     DPCTNLSVFEHILRDCYRRGGTLKRALSCYYSGNFSTGQQPETAFSGTSYIQRIGYPP
                     GSSRYAVPGTREDKSSVAVPLKTAPASDSSRHHVFWPGVVVRGVPAKLRQEKIFIAPF
                     SAPSVRRSFSLSPREEEE"
     gene            4460..4753
                     /locus_tag="L2X49_004166"
     CDS             4460..4753
                     /locus_tag="L2X49_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HBN4799167.1"
                     /translation="MILFRKLKAVVPPVSACTLTTFSATPALADGFQRANTVMQKVST
                     GLHGLAAITITVAVIWIGYKTLWKGESLSQCGYIIIGGILIGGGSEIGALLMS"
     gene            4766..7504
                     /locus_tag="L2X49_004167"
     CDS             4766..7504
                     /locus_tag="L2X49_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HBN4799168.1"
                     /translation="MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLL
                     PVQFLVMKSLTRNEPMRFNLIAVWLRAKGKPVANRLFGATTFMPVEHDDVDIKEFLDA
                     MKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELMGTPFDCESDESLQFGTNQLH
                     GLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFRRNRLFLTV
                     CYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGN
                     AVFSSQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGL
                     EVKEFAEETATGMMDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVS
                     QRLDLDVALDLLTSGKIAYGKHHFSIMVYSPSLESLVADTNEISNALNNIGITPVPAE
                     ISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYPGKRDKAPWGDAMALLRTP
                     SGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYGREDSFS
                     PDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQ
                     LMKILCTRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQEN
                     GLSIRLSQWAQGGEFGWVFDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYR
                     ITSLLDGRRLVIFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIKDD
                     IAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGLDPQARQYVVVKNQFKR
                     GDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAKAL"
     gene            7522..8229
                     /locus_tag="L2X49_004168"
     CDS             7522..8229
                     /locus_tag="L2X49_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBN4799169.1"
                     /translation="MKTRFRTLILACVIASPLAHAGIPVAVDADPMRDVQWAQELKRW
                     METARHYQSQIQAYKDQLATATGVRDIADFVDQAKGLKADLEKLRKPGQALNDLLLSG
                     GSSGQFDALYEKYKIFDTCNTAQSGSYANVCKQQVINKAIQLEQTDEVQNQVSQTLGE
                     INSLSNRVALAKDSKESQDLANSIQLKSVMLNTLTTQWEMSVKAAEKRENALEAERVK
                     QWNQQQLNAPTADLNNL"
     gene            8237..8479
                     /locus_tag="L2X49_004169"
     CDS             8237..8479
                     /locus_tag="L2X49_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HBN4799170.1"
                     /translation="MLSKKIILISTITLCFSIVGCKEEAKTTKWYRDHPDELKLAYEK
                     CQKSGDASDNCKNANEAHYQIKQLNAPTPDLNNLEE"
     gene            8483..>9540
                     /locus_tag="L2X49_004170"
     CDS             8483..>9540
                     /locus_tag="L2X49_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBN4799171.1"
                     /translation="MSGGLFVGVEKYLIGGITDATKRLMMSYSSMMMGLAAASATIYI
                     MWRGYQTLAGKLSTPMEDTMWDIMRMAIILSFVANLGGYLDGVIDAINGIKEGFSGSD
                     NIWQLLDTLWNKAKVLGKTLHDMDNSTYVKDEGMTAQFCVWLGIFALMILAGFVSMVA
                     EVMILLLGITAPIFIFCLMYGFLRPMFNNWLQNIFAGILTILFTALSLRIVVNYLNLV
                     LNKATKVADSSTIVELGAQVCLAGIFAAILVYISAKVAAALAGASATVSMQGLAAVGI
                     GAAAFGAGKLAGGAVKETKRSAQDNIQGWKDANAGKPGKPGGELGYNASKARKYAIEQ
                     MIARNEARRLAQSRESAV"
BASE COUNT         2583 a         2341 c         2270 g         2346 t
ORIGIN      
        1 tgaactatat ttattcttct gcaatcaatg catacataac acaaatatca ctcaggtaca
       61 ctactcaaac cacgctggga tttttcctca agttataatc atcaccccga aaatcattcg
      121 gcatttactc attaaatagt caccccatag gcctgtacat gtttactcag aaatatacat
      181 ccttttctct gtcacaaacc ctctgattaa tcataaataa atacttgtga caccaatctt
      241 tttccttaac ggaacgaatt gttgtgtaga aggagataat attatgtcaa aaaaatatca
      301 gccattgctt ataactcatt atatgtcaac atgggtcact ataacggaag cagttgagat
      361 cactaccaaa gccataaaac aaaaaattac tcctagcgat atttatcgtc atgccttgag
      421 tggcaacatc ctactatcgg tctatttcca gtctcctgtg atacttaaga agatacaaac
      481 ttttaatgga aaaataaaat tcaggcagtt tgagggagac ctacttgata aactatgcat
      541 gcttgacagg gacggtttta tttatggtca aaatttaaga ctctgtacag aagccagata
      601 tgtttgtcct gtgcaacaaa ttatagatac ccccctgata ggctatgaat atgttttgat
      661 tcagcgcata ctggcacgtg aacttaaatt cccctcccca attgtggggg caagaaaaac
      721 taaccatggt attatagtaa gattttcaga ggagcttttt caaattttcg aaacgatgag
      781 ttggaaagaa cgcgtagaaa aacaaatttc cagactacct aaaaacacag cattagatgt
      841 cataaaaaaa ctaacagaag tgacgaccat aaaatataat cacaatgggt gttttcctct
      901 ttatacatta ccgcctgatg cctgttttgt tattcgccac accgaagtcg aaagactaat
      961 taatttatac aaaaaaaggg agagtcatcc catttcacct tcccgtatga caacaccgtt
     1021 atcacgcctg ttttggctgg cctgcaagca caatgatatt atcagcccac tgctgaatca
     1081 tccctataag ctcctttcta tttttgaaca atgggcatca ggcgatggca tcggggaaaa
     1141 actggacgct gaaacactga aaaatgcgct gaagcgtggt tcgccatctt cgacatcctt
     1201 atccggctaa taccatcccc atttgtaatg gccgacaacc ctaaataagg gttttgcatc
     1261 ccttatttag ggctcaccag ctttctctca ggcttccact gtgttttttt attctccggc
     1321 caccgtttat ttcagtcaca ggaggatata tgacatctta tcagttacta cgcctcagac
     1381 aggttgagca aaaaacaggg ctgaagcgtt cacagatata cctttatatg aaagagggtg
     1441 catttccccg ttcaataaaa ataggccctg ctagtgttgc ctggctcgag tctgagattg
     1501 acgagtggat caacaaaaag ttatctgacc gataaagacg caatgaggag aagatgatga
     1561 acattaatat cccggcactc ataaacttcg atcctgcagg tataaaaaaa agcacctgca
     1621 tgtatatttc agacctgccc ggtattgtgc tatatgacac tataccattc actgaatatt
     1681 cagctgaatt actgagtact tattcagtta aggagtcaat ttcatgcttc catccctgaa
     1741 tcatattata ttaaccgttg ctcttcaggc gctacgggaa ggaaatattc atcactgcga
     1801 aactatggga ttcacttacg atgaaatgaa tttgctcggt tgcttatcaa taaacgatct
     1861 tatcaccctc agtcaggcac ctttaccgct tgtcgatatt actatccgcc atgatgtcct
     1921 gcaaaaatta ctggcatcat cccacgagga aaacaggcgt caggaacaac tgaacagagc
     1981 tgtcaggctg gggggctcaa tcgcactgat gaaccgttat ttcggggttg gctccagaga
     2041 aacctgtgca cgccgccggt tactcggtgt ctccgttcca aacgggagaa cacccattcc
     2101 ggatgaagaa accgatgctg caatctggca tcagtggcaa aaatatcggg tcgaaaacat
     2161 tgattccccg gaggcgctgg acgccatgat gctggttaca gaaaaccttt catcgtgtac
     2221 tgccccctcg ctgaccgttg tctggaactg catcacgcat tgcgaaagaa agaacacggt
     2281 aaggagaata caacgtgcca gatgaaatcg atcgcgatca ggcgtttaat gaacggtgcc
     2341 tagaagcact gattgaacaa agccggttaa gacccacacc gacaccttcc ctgcagcact
     2401 gtcgattatg cggcaaggct attccggaaa aacgccgtca gacgctgccg ggtgtaacaa
     2461 cctgtactga ctgtcagtcg atactggaga aacgcagacg ttaaatctgt atgtgcggag
     2521 gagaatagct ccgcacattt taatatgcac cagccggctt gttttcaggt tcccgtgcct
     2581 ccatgtcatg gatgaataac ctgctcacat ctcctgctgt ggcaatcctt ttttacagtc
     2641 agtacaccag aggtcagaca tgaacaaacc ggcaaacaag atggaattcg tcagaaaact
     2701 gcggtaaata aatgacggca gcctgctgga aaaggttgtc gggcatatgg aagaaaagct
     2761 cacagggaat gagcttgaaa tgctttatgc cgcagcagat tatcgccgcg cagagattgt
     2821 aatgaaccag tactttgata aggttcccgg taatgcctgg aaatatgtca gataactctg
     2881 tgttttttat gcaacaggta cagacggata ccactgcggc atgaaaacga atttcaccac
     2941 ccggcccggt aagtatcggc ctaagtggcc aggaaacgtt taaccagatt aaagaccgtc
     3001 ccttacaaca aaagggacta ctgtattcct gtctttcacg ccttacacat tattgtcgtt
     3061 accaaattca gccacagaga cagcatacaa ccccatctga aatataaaga aagtcttcct
     3121 ccggatgttt aatttaacct gataataatc aacaaatatt attatcagcc cgcccgtcat
     3181 cattcatgca cacacacacc gtgccagtaa tacgttaatt tcacatactt ttttatcatt
     3241 ccttccggta tttattttcc cgttaaaatt tcattacccc tcctgcatcc tgaccatgct
     3301 ctttaacaat atggagcggg ctgtcacaca accataattc agggtgccgc tgaatgttct
     3361 cgctgacccg ttaaaaccgg aatgcaggat cggaatgtga tcgaagaaat catcttcaac
     3421 aattttaatt aaacgaggac actatgagaa agcctgttta ttacgcgtat gttgcccagg
     3481 aaagtcatcc ggactctcag ggtgagaaaa atacctggtg gacaaaggtc ggagtcgcct
     3541 ttccccacca cggtaaatcc ggtctgaata ttgtcctgac gcccggtatt gccgtttccg
     3601 gcaaactggt cctgcttgag ccccgggagg aaaataatcc accacagaat acgggcccca
     3661 tcgaaaccgt gtcgtaacca cccttaattc tgttcccacg gcatgcagta tcagcagacc
     3721 ctgcatgtca cttgtttact gaggtcacca tgctttccac cacagccttt ctggcgatag
     3781 ccatgcagtg cgccgccagc attcatccgt ccacggcatt cgacgtggca cgggtggaat
     3841 ccggttttaa cccttatgcc atcgctgaga ttgtcccgaa ggccggcccc tctttcggcg
     3901 atcaacacat tatttctcac ctgccagcca gccggggaga tgcagagcat attctccgtc
     3961 gcctgtcagc acaggatcgc cgttactctg tcgggctgat gcaaatcacc agcaccaatt
     4021 tccgccatta tggtgtgtcc gccacagagc tacttgatcc ctgtaccaat ctgtccgtct
     4081 tcgaacacat tctcagagac tgctaccggc gcggcggcac gctgaaaagg gcgctgagtt
     4141 gttattattc cggcaatttc agtaccgggc aacaacctga aacggcgttt tccggaacga
     4201 gctatatcca gcgaatcggt tatccgccgg gatcatcccg ctatgcggtc cccggaaccc
     4261 gtgaggacaa atcatcagtt gccgttcccc tgaaaaccgc ccctgccagt gactcatccc
     4321 gtcaccacgt gttctggccc ggagtcgtcg tccgtggcgt ccctgcgaaa ctccgccaag
     4381 aaaaaatctt catcgcacct ttctccgcgc cgtcagtgcg ccggagtttc tccctgtcac
     4441 cccgtgagga ggaggaataa tgatactttt ccgcaagcta aaagctgtgg tcccccccgt
     4501 ttcggcctgt acgctgacga ctttttctgc aaccccggca ctggccgacg gatttcagcg
     4561 ggcaaatact gtcatgcaaa aagtgtcaac cgggctgcat ggtctggcgg caatcacgat
     4621 caccgtggcc gtgatctgga ttggctataa gaccctgtgg aaaggcgaga gcctgtcgca
     4681 gtgcggctac atcatcattg gcggcatttt aattggcgga ggcagtgaaa tcggcgctct
     4741 gctgatgagc taagaggctg aacatatggc aaagctgctc aaggcaatga aacgcccggc
     4801 ggcgctgtgg ggggtgccaa tggtgcccct gctggctgtc acgggtgtaa ccatcatcgt
     4861 tgctatctgg acttccgtcg ctctcctgtt cttacttcct gtgcaatttc tggtgatgaa
     4921 gtcccttacc agaaatgaac cgatgcggtt caatctgatt gccgtatggc tgagagcgaa
     4981 gggaaagccg gtagcaaacc gtttatttgg cgcaacaaca tttatgcccg ttgagcatga
     5041 tgatgttgat atcaaggagt ttctcgacgc catgaaacta aatcagtgtg ccaccatcaa
     5101 gaagtacatc ccgtactcat cgcacattca tcagcatgtg gttcgttccc ctaagagcga
     5161 tctgtactgc acctgggaat tgatgggaac gccgttcgac tgcgagtctg acgagtcgct
     5221 ccagttcggg acgaaccagt tacacgggct aatccgctca ttcgaaggta tgccagttac
     5281 tttctatatc cataacgacc gcaatacgtt taccgacaat cttcataaag attccggcaa
     5341 cccgtatgcc gatgaagtta gccgtcttta ctacgcttct gtcggtctgt tccggcgcaa
     5401 ccgcctgttc ctgacggtct gctatcgccc gtttgtcagc cttgaaaaag cagaacgcaa
     5461 acggatgaaa gacagccaga aactaaagga gctggacggt gctttgctgg aaatgctgga
     5521 aataaaaagc acgctcgata ccgcgctgtc gcgctatggt gcgcgacctc tcggtacgtt
     5581 taccgaagga aacgcggtgt tttcgtcgca actggctttc tatgaatatc tgctaacgca
     5641 ccagtggcga aaagtgcgcg tcacccgtac cccggcctat gacgtgatgg gggcggcggc
     5701 gttgttcttt tcggctgaat ccgggcagat aaaccacgcc aacggtacgc aatatttccg
     5761 ggggctggag gttaaagagt tcgccgaaga gaccgccacg gggatgatgg actcccttct
     5821 ttacgccccc tgcgattacg tgatcacgca gtcctataca tgcatgtcac gcgaggaagc
     5881 gaagaaagcc attaagcgta cccgccgcct tttgatgagt gccgacgacg atgctgtttc
     5941 ccagcggctg gatctggatg tggcgcttga cctgctgaca tcggggaaaa tcgcttacgg
     6001 caagcaccat ttttccatca tggtgtattc cccgtcgctg gaaagcctgg ttgctgatac
     6061 gaatgaaatc agtaacgcgc tgaacaacat agggataacg cctgttccgg cagaaatatc
     6121 gctttcagcg gcgtatatgg cgcaactgcc aggcaactac aacctgaggc cacgtaaagg
     6181 ggagctgagt agccagaact ttgtggagct ggccgcgctc cataatttct atccgggcaa
     6241 gcgcgataaa gccccctggg gggatgcgat ggctttactg cgcaccccgt caggcgatgg
     6301 ctactatatc aacctgcata acacactggc agataaggac gaattcaacg agaagaaccc
     6361 ggccagtacc tgcatcctcg gtacgaacgg ttcgggtaaa accatgttga tgacgttctt
     6421 tgagatcatg cagcagaaat acgggcgcga ggatagcttc tccccggatg ccaaaaccaa
     6481 acggctgaca accgtttacc tcgataaaga ccggggcgca gagatgaaca tccgggcgct
     6541 gggtggccgt tactatcggg ttatcagtgg tgagtctaca gggtggaacc cgttctcgtt
     6601 gccagcaacc aaacgcaata tcaatttcat caagcaactg atgaaaatcc tttgtacccg
     6661 taacggctcg acgatctccc cacgcgatga acgccgtctg agtgatgcgg tcaatgctgt
     6721 gatgaatgac gagccgcaat atcgcgtcta cggcattacc cgaatgctgg aaaatttgcc
     6781 tgagccggct acgaaagaag cccaggaaaa cggccttagc attcggttgt cacagtgggc
     6841 gcagggcggt gagttcggct gggtgtttga taacgagtct gacacgttcg atatcagcaa
     6901 ctgcgataac ttcggcatcg acggaacgga gttcctggac gatgcaagcg tatgtgcgcc
     6961 aatctcgttt tatctgctgt accgcattac cagcctgctt gacgggcgac gcctggtcat
     7021 tttcatggat gaattctgga agtggttacg cgacccggtg tttaaggact ttgcttataa
     7081 caagctgaaa accatccgta agctgaacgg gatgctggtc gtcggtactc aatccccggc
     7141 agagatcatc aaagatgata tcgccccggc agtgattgag cagtgcggga cacagattct
     7201 ggcggcgaac cccaacgccg accgcgcaca ctatgttgac ggtatgaagt ttgaaccgga
     7261 agtgttcgac gtggtgaaag ggcttgaccc gcaagcccgc cagtatgttg tggtgaaaaa
     7321 tcagttcaag cgcggcgata ccaaacgttt tgccgctcgt gtcacgctcg acctgtcggg
     7381 tatcggcaga tacaccaaag tcatgagcgg cgatgccccc aacctcgaaa tattcgaatc
     7441 gatttaccgg gaaggaatgc agcctcatga atggctcgat acctacctgg ctaaagcgct
     7501 ctgacccaaa ggagatatac gatgaaaact cgctttcgta ctcttattct ggcttgcgtc
     7561 attgcaagcc cactggctca tgctggtatt cccgtcgctg ttgatgctga ccctatgcgg
     7621 gacgtgcagt gggcgcagga gttaaaacgc tggatggaaa cggccagaca ctaccagtca
     7681 caaatacagg catataaaga ccaactggcg acggccactg gcgttcgtga tattgctgat
     7741 tttgttgacc aggccaaagg cctgaaagct gaccttgaaa agctgcgtaa gcccggacag
     7801 gcactgaatg acctgcttct ctccggcggt tcctccgggc agttcgatgc gttgtacgag
     7861 aagtataaaa ttttcgacac ctgcaacact gcgcaatcgg ggagttatgc caacgtatgc
     7921 aagcagcagg tcatcaacaa ggctatccag cttgagcaaa ccgacgaggt tcagaaccag
     7981 gtaagccaga ccctggggga aataaacagt ctctccaaca gggtagcgtt agccaaagat
     8041 tcgaaagaat ctcaagacct tgcaaacagt attcagctaa aatctgtcat gctgaacacg
     8101 cttaccaccc aatgggaaat gagcgtgaaa gcagcagaga agcgcgaaaa tgcgctagaa
     8161 gcggagcgtg ttaagcagtg gaatcagcag cagttaaacg cgccaactgc tgaccttaat
     8221 aatttatgag gtatttatgt tatcaaaaaa aattatttta atttcgacga tcaccctgtg
     8281 cttttctatt gttggatgca aagaggaagc taaaacaaca aaatggtata gggatcaccc
     8341 tgacgaatta aaactagctt atgaaaagtg ccaaaagagt ggtgacgctt ctgataattg
     8401 caagaatgca aatgaagccc actatcaaat caagcagcta aacgcaccaa ctccagactt
     8461 aaataattta gaggagtaat atatgagtgg cggtcttttt gttggggttg agaaatacct
     8521 gataggagga attacagacg ccacaaagag attgatgatg tcgtactctt ctatgatgat
     8581 ggggttggca gcagcgtcag ctaccatcta catcatgtgg cgtggatacc agactttggc
     8641 tggtaagcta tctacgccta tggaagacac gatgtgggac atcatgagga tggcgatcat
     8701 cctgtccttc gtggctaatt taggcggcta cctcgatggc gttattgatg ccataaacgg
     8761 aataaaggag ggtttttctg gtagtgataa tatatggcag ttattagaca ccctatggaa
     8821 taaggctaaa gtgttaggga aaacgctaca tgacatggat aattctacat atgtaaaaga
     8881 tgagggaatg actgctcaat tttgtgtatg gcttggaata ttcgcactta tgattctagc
     8941 gggctttgtt tcaatggttg ccgaagtaat gattctacta ttaggaataa ctgcacctat
     9001 tttcatattc tgccttatgt atggtttcct ccgccccatg ttcaataact ggttgcaaaa
     9061 tatatttgca ggaatattaa caattctatt taccgctctc tcattgagaa ttgtagtgaa
     9121 ttacttgaat ctcgttctta ataaggcgac gaaggtagca gacagttcaa caattgttga
     9181 acttggtgct caagtctgcc ttgcaggtat tttcgctgca atactggtgt atatttccgc
     9241 taaggtagcc gcagctctgg ccggggcaag cgctaccgta tccatgcagg ggctggccgc
     9301 tgtaggtatc ggtgcggctg cttttggtgc cgggaagcta gccgggggtg cagttaaaga
     9361 aaccaaacgt tccgcccagg acaacattca gggctggaag gatgcgaatg caggtaagcc
     9421 cggcaagccc ggaggtgagt taggctataa cgcatctaaa gcgcggaagt acgcgattga
     9481 gcagatgata gcccggaacg aagcgcggag gctggcacag tcgagagaat ccgcggttaa
//
LOCUS       DAFNBJ010000177         9392 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000177 DAFNBJ010000000
VERSION     DAFNBJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.177"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..1044)
                     /gene="trhU"
                     /locus_tag="L2X49_004171"
     CDS             complement(<1..1044)
                     /gene="trhU"
                     /locus_tag="L2X49_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhU"
                     /protein_id="HBN4799172.1"
                     /translation="MKKLKKLLLEILIAVMLSLSLPAMTMAADTGVPGAMCQSAGVWQ
                     GLIKNICWSCIFPMRIMGIGAAPEGAAPSRPGCYCTDQNGIPEIGWQLSFFQPVKIVE
                     VVKSPWCSPFLEGTMLQKSQFDIGKSNTNQPMTATEAGFYDVHLWEFPIMTMLKLLVI
                     GECTAEPYVDASLTYISEVDPMWESDLLTLVLNPEAVVFANPIASMVCAADCVAVTAG
                     KDNLAAYFCAGCDGNLYPLTGHIYANDDAVRTSSLITQRLLTKLHRQGMLMRTMGADA
                     MCEKTWEYFTPRSQYRLSMLYPTPEANGPDCCHRLGDSVHDWSTLKGGRKKIGIDNYV
                     YMLWRYNDCCVRYI"
     gene            complement(1189..2697)
                     /gene="trhW"
                     /locus_tag="L2X49_004172"
     CDS             complement(1189..2697)
                     /gene="trhW"
                     /locus_tag="L2X49_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhW"
                     /protein_id="HBN4799173.1"
                     /translation="MRSFELKTTIKPLASCLAFYALISTAVASPQYQQVADDIARQAQ
                     VLGAQIPVPHASEGPLPSGSLDSPDTKKYIRQAEAMKKNGDLSQQTNRGYVPGMNADS
                     VQAVIDHTQAIRAQSNNSEAVNDIIRRRDEIQGNSQLSESALKSVENKPEVMRSQSSN
                     IEKMFGSSGISAADFERKIDSSREEVLSTENGITIFASFSLPDYVLEDLLRTASEHKA
                     RVVFNGLKKGTTRLPETQAAINQLVVKGKFDSPLITIDPDAFNQYQITQVPTIISREQ
                     SRFAKMVGSFNVDFFQRELARKPDQDLFPIAGTTYPVEEKSIIKELEERAQKYDWDGA
                     KKRAVADTWKNQYMVNLPPAQEHKEWLIDPTIRVTQDVKDKQGRVIASAGELINPLAR
                     FPQNLTMIIFDPMNPGQLEWAEKQYRQRLGSGQVMPMFTRIKQENGWDHLNDLREKFN
                     GKVFKVNEQIIARFQIKNTPALISTEKEKFRITQFSEAEIRGIGTKIAAEEN"
     gene            complement(2684..3196)
                     /gene="trhF"
                     /locus_tag="L2X49_004173"
     CDS             complement(2684..3196)
                     /gene="trhF"
                     /locus_tag="L2X49_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhF"
                     /protein_id="HBN4799174.1"
                     /translation="MREYIPKKRGLLSFDWYVPINLGILILVMLIFFNRFTFGYGLLN
                     GCLPADFYMIDLKDKSVRSGNIIAFHMPKSVRFIRENEKVIKIVAGVGGDRLRVTLDG
                     VYNGNKFYKANARRISVKYGIPAETIERELTIPEGEVFLIGQTDHSWDSRFWGPVKLT
                     SVIGKTYAIF"
     gene            complement(3471..7769)
                     /gene="rsp"
                     /locus_tag="L2X49_004174"
     CDS             complement(3471..7769)
                     /gene="rsp"
                     /locus_tag="L2X49_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein RSP"
                     /protein_id="HBN4799175.1"
                     /translation="MRKSGLCLTLLFSLIASIKSVQAEAIMISGKLQADMPAVTFNPG
                     PGDFVAFVNNNTITASGTGNACNVTVDDRATSSVDNLVCFFEWLPNTSGFTANGFNLT
                     GIPNAAGELKLPYKISYFSGSERQKVEVVNGEYTVNALVPVKPTITGLKSSMNGVVQD
                     GYTLKSYLKDEAIRNVTVSVEPRNYVQYISIGSGSACEVAIGGNGCTIDVGSIKVSDS
                     DLLMGSREITVTANSKNNYFAPPEAQKLTLTWDYRPPVVDHTLWNFTDEAKTISIGGQ
                     EIYTGARTVAVAVKVPQEETEGEWWLPTAMTLTMNPDGVFKPTTKVTLEDGTVIDFKQ
                     SWATPLRRTLQPVSGPQKVGDEYLYIFDLTDLTNGSYAASFTVENNSKNTSTYNEPES
                     KLMLSDNPTLMVLKDGAALAKRAPVYFLNEIIVAAFQGQAGVADIKAVTIDNKLVELT
                     PTNHKGIYYLPVGDDLEVNADHEITVIAENLYGKIVTFNTTFTYQPTGFTLKNFEKDI
                     TLYSRVRQYTDLMSQTAGDKCTLFTTEENALAYLEWYGDKSDITACYPQWNNVPDGLE
                     FYFKGRTPGLTGFFNKTGDNLLDYQVYMINGKGSKAVSSRNRHVLITQLPYNPVLTYK
                     KNKVLTGINDNTALAYTTGGEAARLLAKVVPADVTVYVSQNGGESVKTTFKNRSSNND
                     ATTFVQRLKVASAPLWTKNVFDIAVEYSKEPTMRTVDTLNVYTVPDFNIRAAMEIEDK
                     KTATTMELPLKVSVGRYNNSTRKSSFSRTTMGDWDVTIYAQKSVYGKDPETGRYKTTY
                     ERTPLTEALPVNDAGVVETKIKIENMDLGNVRLVGIAKVRSPFADFELTRETSAVSIR
                     VYKGEELEGNLSKSLIIGRIPLSTLVNFKSASTANSDALAPTEWQESSDNGQTWTMLQ
                     DMTGKRSISIRKTEVGKWLYRAKMTNKFTSKESYSDVLTVVTYKQPKLSIDVAEILEG
                     DDLPVTLLDNDEPIPQGTAEVMWSEDKVNWVQGDTTYNVSAADTLPPTIYARMRYLDS
                     DELAEESSWKETSARLTVAKPKRLSVSVSGISSVEVGSKVTLDGKYTNPNSRFKNGND
                     VIEEWTAPDGQKIRGANLSITLTEQMLDKQGYAAFEYSAWLADSKDSTISTRKVSVKS
                     WVYKFPEMKVSTKLKYTMAPSTLHVALSGIKDGGYLGVTYSREWIYDKDNISVLKDDG
                     DTKEFAIAKPGKYTLVIVFRDNRNNEQRIENTFVVDEQTPMTVEMTPKFSNKFMRAPL
                     DVTLRSNIKLAHSADSIDTVTYKVNGEVVPGSKNYWAQLISGLTEKKYEVTLDVVSKM
                     GQRGTASVDFEVVKNAAPQCALSYTESNMSWSFTNKCIDTDGKLVRYEWYINDELRNV
                     FGSTATLSKNLNRGKQDIRVVAYDDSGDSATQRTTVYGLDEGASKSVDSVETSQ"
     gene            complement(7889..8452)
                     /locus_tag="L2X49_004175"
     CDS             complement(7889..8452)
                     /locus_tag="L2X49_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HBN4799176.1"
                     /translation="MTMSDLSATGAKPQRVRVFSQSVAMAFEVNLDKSRSADNSAFVT
                     ETEKYYLSLQFAPPSDNREYGWNSEGSILMKLSQNEAMALASVFLRIKPTLKFEKRKT
                     THRTHQAYKNINIGPNDRGGLLVSASIVPVKMGTFKPLNYNLPVTQMDCVSTGLFLLG
                     FLTLKMPWVSSESIITALRLSESKSGQ"
     gene            complement(8449..8883)
                     /locus_tag="L2X49_004176"
     CDS             complement(8449..8883)
                     /locus_tag="L2X49_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HBN4799177.1"
                     /translation="MKVNFKKVIPLLMVGLTVAGSYSIFNAKPSKPDLYDFTGKVLKT
                     TSVFQPCDKESTPSLNIQIADNGSVHINGVASKVTFVERVPGNEIAVKCTGLQVKNSR
                     LVHTSSYTMIISEGKGGFVISDLTHTKDNEVMSGTWFFKKRV"
     gene            complement(8873..9325)
                     /locus_tag="L2X49_004177"
     CDS             complement(8873..9325)
                     /locus_tag="L2X49_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transfer repressor"
                     /protein_id="HBN4799178.1"
                     /translation="MLSRNSLIHGLRRDQLIEVLSISEFPVVLVENPFIQPKSTGKKP
                     VIFNIDEFHVSIAPISSLKYDWEWAPIDTILIEVVIPPAEADLVSAENDFLRDAGIGH
                     IQCEPGGVSIRRTVTFVGGITADNLLYQLRLMCVSALHLIGEELEDES"
BASE COUNT         2415 a         2282 c         1958 g         2737 t
ORIGIN      
        1 tatatacctg acgcagcaat cattatagcg ccacagcatg taaacatagt tatctatgcc
       61 gatttttttc cgtccacctt taagcgttga ccagtcatgg acagaatcgc ctaggcgatg
      121 acagcaatcc ggaccattcg cctccggcgt tgggtaaagc attgaaagtc ggtattgaga
      181 tcgaggagtg aagtattccc acgttttttc acacattgcg tcagctccca ttgttctcat
      241 aagcattccc tgcctatgta actttgtcag tagacgctga gttataagtg agctggttct
      301 gacggcatca tcattcgcat agatgtgtcc agttaacggg taaaggttcc catcacaccc
      361 ggcacaaaaa tatgcagcca aattatcctt cccggcagtc acggccacgc agtcagctgc
      421 acataccatt gaggcgatag ggtttgcaaa taccacagct tcggggttta gcacgagcgt
      481 caataggtca ctttcccaca ttggatctac ttcgctgata tacgtcagac tggcatcaac
      541 ataaggttct gctgtacatt caccaataac cagcaatttc aacatcgtca tgattgggaa
      601 ttcccagaga tgtacgtcgt agaatccggc ttctgttgcg gtcataggct ggttagtatt
      661 gctcttgccg atgtcgaact gtgatttttg cagcatggtt ccctctaaaa atgggctgca
      721 ccacggactt ttcaccactt caacaatttt tactggttgg aaaaaactta gctgccatcc
      781 gatttctggt atcccatttt gatccgtgca atagcaacca gggcgtgagg gggcggcacc
      841 ttcaggggct gctccaattc ccatgatccg catcgggaag atacaactcc agcagatgtt
      901 tttgattagg ccctgccata ctcccgctga ctggcacatt gctcccggga caccggtatc
      961 tgcggccatt gtcattgcag gcagcgagag cgacagcatg acagcgatca atatttccaa
     1021 aagtaacttt tttagcttct tcaccggcac gctcactatt tagtaatgaa tgttgtcacg
     1081 ctttccatat acgcaaccac tttgttaaaa cactctctca tctggtccgg gtaagttagc
     1141 gtcagccaag tggccagcag cgccccaaaa ataaatccta ttttcatatc aattctcctc
     1201 tgccgcgatt tttgttccaa taccacgtat ttcagcttca ctgaattgcg ttatacggaa
     1261 tttctctttt tcagtggaga tcagcgctgg cgtgtttttt atctggaaac gagcaataat
     1321 ttgctcatta actttgaaca ccttcccatt gaatttttcg cgcagatcat tcaaatgatc
     1381 ccatccattt tcctgtttga tacgcgtgaa catcggcatc acctgaccgc tgccaagacg
     1441 ctggcgatat tgcttctctg cccactcaag ttgcccaggg ttcattgggt caaaaattat
     1501 cattgtgagg ttctgcggga acctggccag tgggttaatg agctcgccag cagaagcaat
     1561 gacgcgaccc tgcttatctt taacgtcctg agtaaccctg attgtcgggt cgattagcca
     1621 ttccttgtgc tcttgagcgg gaggcagatt aaccatgtac tggtttttcc aggtatcagc
     1681 gactgctctc ttcttggcgc cgtcccagtc gtatttctgt gctcgctctt cgagctcttt
     1741 aataatgctc ttctcttcaa ccggataggt cgtaccagca atcgggaaaa gatcttggtc
     1801 gggctttctg gccagttcgc gctggaagaa atcaacgttg aaagagccga ccatttttgc
     1861 aaaccgggat tgctcacggg agattattgt cgggacctgc gtgatctggt actggttaaa
     1921 tgcgtccggg tcaatcgtaa ttagtgggga gtcaaacttc cccttcacca ccagctgatt
     1981 gatagctgcc tgagtttcag ggaggcgtgt agtgcctttc ttcagaccat taaaaaccac
     2041 tctcgcctta tgctctgacg cggtacggag tagatcttcc aggacgtaat caggcaagct
     2101 aaatgacgca aagatagtga taccgttttc tgtcgagagc acctcttccc gactgctgtc
     2161 tattttacgc tcgaagtcag cggcggagat tcctgatgaa ccaaacatct tttcgatatt
     2221 cgaagactga ctgcgcataa cttctggttt attttcgacg gatttcagtg cagactcgct
     2281 gagctgtgaa tttccctgaa tctcatcacg tctgcgaatg atgtcgttaa ctgcttccga
     2341 gttattactc tgagcgcgga tagcctgcgt atggtctata actgcctgca cactgtcagc
     2401 attcataccc gggacataac cgcgatttgt ttgctgactg aggtccccgt ttttcttcat
     2461 agcttcggcc tggcggatgt atttcttggt atcaggagaa tccagagaac cagacgggag
     2521 cggaccttct gatgcgtgtg gtaccggtat ttgtgcgccc agaacctgag cttgccttgc
     2581 aatgtcatca gcaacctgtt gatactgggg gctagccaca gcggtggata ttaaggcgta
     2641 aaatgccaga caggaggcca gaggcttaat agttgttttt aattcaaaag atcgcatatg
     2701 ttttcccaat aactgaagtc aacttaaccg gcccccaaaa acgtgagtcc catgagtggt
     2761 ccgtttgccc aattaaaaag acctcccctt ccggaatagt taattctctt tcaatggtct
     2821 cggcaggtat gccgtatttc acagaaatac gtctggcatt ggctttataa aatttgttcc
     2881 cattataaac accatccaat gtgaccctca ggcgatctcc gccgactcct gccacaattt
     2941 taataacctt ctcattttca cgtataaaac ggactgattt tggcatatga aaagctataa
     3001 tattccctga ccttaccgat ttatccttca gatcgatcat gtaaaaatca gcaggtaaac
     3061 atccatttaa tagtccatat ccaaacgtaa atcgattaaa aaaaatcaac ataaccagta
     3121 tcaggattcc gagatttatt ggcacgtacc aatcaaaact cagtaaacca cgcttcttag
     3181 gtatgtattc tctcataacc tacttctctc agaatcagaa agcaaaatgg actgtctttt
     3241 acattacctt aaaacctatt atccatgttt tggtacttcc ttatttcagc aaagccagct
     3301 atttctgatt atttaccgcc ttttttttct ttcatcccgt ctttgatttg tgattgtcct
     3361 ggccgctgtc ttactcatgt aaagtaactt tcccgacgtt accccatcaa aaagtgaaaa
     3421 acaaaaaagc cgggacattt ctgccccggc tgttatttca ttagcttgcc ttactgcgag
     3481 gtttcaactg aatctacgga ctttgacgcg ccctcatcca gaccataaac ggtcgtacgc
     3541 tgagttgctg aatccccact atcatcatac gcgacaactc gtatatcttg cttcccacga
     3601 ttaaggtttt tggacagtgt cgcagtgctg ccgaaaacat tccttagctc atcattgata
     3661 taccattcat atcgaaccag cttgccatca gtgtcaatac atttgtttgt gaaactccag
     3721 ctcatattag actctgtgta gcttaatgca cattgcggag ccgcgttttt aacaacctca
     3781 aaatcaactg atgcggtacc tctttggccc attttactga caacatccag cgtgacttcg
     3841 tatttcttct cagtaagacc agaaattaac tgagcccaat agtttttact accaggaaca
     3901 acctcgccgt ttactttgta tgtcacagta tcaatactgt cagcagagtg cgccagttta
     3961 atgttagatc ttagcgtgac atcaagaggc gccctcatga acttattaga gaatttcggt
     4021 gtcatttcta cagtcatggg cgtttgctca tcgactacga aggtattttc aatacgctgt
     4081 tcgttgttac ggttatcacg gaaaacgatt accaacgtat atttccccgg cttcgcaatt
     4141 gcaaattctt tcgtatcacc atcatctttt aaaactgaga tattgtcctt atcgtagatc
     4201 cattcccgag aataagttac cccaagatag ccaccgtctt ttattccgct cagcgcaaca
     4261 tgtaaggtcg acggggccat ggtgtattta agctttgtac tgactttcat ctcaggaaac
     4321 ttatacaccc aagatttaac agatactttc cgcgtggaaa tagtactgtc cttactgtca
     4381 gcaagccatg cactgtattc aaatgccgca tatccttgct tatccagcat ctgctcagtc
     4441 aaagttatcg acagattcgc acctctgatt ttttgtccgt caggggcagt ccattcttca
     4501 atgacgtcat ttccgttctt gaatcggctg ttcgggttag tgtatttacc atctaaagta
     4561 actttacttc ccacctcgac gcttgaaata ccacttactg aaacagatag gcgcttgggt
     4621 ttagcgacgg ttaaccgggc agacgtctct ttccaggatg actcttcagc caactcatca
     4681 gaatccaggt aacgcattcg ggcgtaaatg gtcggaggta gcgtatcagc ggccgatacg
     4741 ttataggtag tgtcaccctg aacccaattc actttatcct ccgaccacat cacttcggcc
     4801 gtaccctgcg gaattggttc atcgttatcc agcagcgtga caggcaaatc atcgccttcg
     4861 agaatttcag caacatcgat gctcagctta ggctgtttgt acgttacaac cgtcagaacg
     4921 tcgctataag actctttcga cgtaaacttg ttggtcattt ttgccctgta aagccattta
     4981 ccaacctcag ttttcctgat cgaaatgctt cgcttccctg tcatgtcctg aagcatagtc
     5041 catgtctgtc cattatcaga agactcctgc cactcggtag gcgccagcgc atcggagtta
     5101 gctgtactgg ccgatttgaa gttaacaagc gtactgagcg ggatacgtcc gataatcaga
     5161 ctctttgaga gattaccttc tagctcttcg cccttataaa cacggatgct aactgcactg
     5221 gtttcccggg tcagttcgaa atctgcgaaa ggcgatcgta ctttagcaat cccgacaagt
     5281 cgcacgttcc cgaggtccat attttctatc ttgatcttgg tttcaaccac cccggcatca
     5341 ttcacgggca gtgcttcggt taggggtgta cgttcatacg tcgttttata tcggcctgtt
     5401 tctggatctt ttccataaac acttttctga gcgtaaatgg ttacatccca gtcccccatt
     5461 gttgtccgac taaatgaact tttgcgggtt gaattgttat agcggccaac agacaccttc
     5521 aatggcaatt ccatcgtggt agcagtcttt ttgtcctcaa tttccattgc cgctctaatg
     5581 ttaaagtcgg gtaccgtata cacattgagc gtatcaacgg ttcgcatagt cggttccttc
     5641 gagtactcga cagctatgtc gaagacattc ttcgtccaca acggggctga tgccacttta
     5701 agacgttgaa caaacgtcgt tgcatcgtta tttgatgaac ggttcttaaa cgtcgttttt
     5761 acgctttccc ccccgttttg tgaaacatag actgtcacat cagcaggaac cacttttgcc
     5821 aatagacgag ctgcttcccc gccagtggta tacgccagtg cggtgttatc gttaatcccc
     5881 gtcaacacct tgtttttctt gtaggtaagg accgggttat aaggtaactg cgtaatcagc
     5941 acatggcggt ttctggacga tacagcttta gagcccttgc cgtttatcat atagacctga
     6001 tagtcgagca ggttatcgcc tgttttattg aagaaccctg tcaggcctgg cgtccgacct
     6061 ttaaagtaaa attcaagccc gtcaggcaca ttgttccact gagggtaaca ggcggtgata
     6121 tcagatttgt caccatacca ttcaaggtat gccagtgcgt tctcttctgt agtaaagaga
     6181 gtgcatttat caccggccgt ctgactcatg agatcggtat actgccgaac tcgtgagtac
     6241 aatgtgatgt ctttctcaaa gtttttaagc gtgaatcctg ttggttgata cgtgaaagta
     6301 gtattaaatg tcacaatctt gccgtacagg ttttcagcta tgaccgtgat ttcatggtca
     6361 gcattcactt ctagatcatc cccaaccggc agataataaa tgcctttgtg gttcgtgggg
     6421 gtgagctcaa caagtttgtt atctatcgta acagctttaa tatccgcgac tccggcctgg
     6481 ccctggaatg cggcaacaat aatttcattt aagaaataca caggagctcg tttggccaga
     6541 gctgcaccgt ccttaagcac catcagtgtt ggattatcag aaagcatcag tttgctttca
     6601 ggctcattgt acgtagaggt attcttgcta ttgttctcta ccgtgaacga ggcggcataa
     6661 gaaccattcg tcaagtctgt taagtcaaag atatacagat attcatcgcc gactttctgt
     6721 ggcccgctca cgggctgaag agtacgacgt agcggtgttg cccaggattg cttaaagtcg
     6781 ataaccgtac catcttctaa agtaactttt gttgtcggct taaacacccc atcaggattc
     6841 atcgttagcg tcatagcggt tggtaaccac cactcaccct cagtttcttc ctgaggcact
     6901 ttgacggcta cggccaccgt tctagctcct gtatagatct cctgtccacc aatacttatt
     6961 gttttagctt catccgtgaa attccagaga gtatggtcaa caacaggagg acggtaatcc
     7021 caggtcagcg ttagcttctg tgcttcagga ggggcaaagt aattattttt cgaattggca
     7081 gtgaccgtga tttcccgtga acccatcagc aaatccgaat cacttacttt gatgcttcca
     7141 acatcgatcg tgcagccatt cccaccaatt gcgacttcgc aagctgatcc agacccgata
     7201 gagatatatt gcacataatt gcgaggctct acggagacag tgacattcct aattgcttca
     7261 tccttaagat aggatttaag agtgtaacca tcctgaacca ctccattcat agacgatttc
     7321 aggcccgtta tcgtcggttt aaccggtaca agagcattaa ctgtatactc cccattaacc
     7381 acttctactt tctgcctttc tgatccagaa aaataagaaa ttttatacgg cagttttagt
     7441 tctcctgccg cattgggtat ccctgttaaa ttgaatccgt ttgctgtaaa gccagaggta
     7501 tttggaagcc attcaaaaaa gcagaccaag ttatctacgc ttgatgtcgc acgatcatca
     7561 accgtcacat tgcatgcgtt gccagtacct gaagcagtaa ttgtattatt gttgacgaat
     7621 gcaacaaaat cgcccgggcc gggattaaac gtgacggcag gcatatcagc ctggagtttt
     7681 ccggaaatca taattgcctc agcttgtacc gatttaatcg aagcaattaa agaaaacaac
     7741 agggttaaac ataaacctga ttttctcatg acgaacacct gtttttttaa gcagaattag
     7801 aatcactcta cgttaaaaaa ttctgaaatc ttgttcgtaa agaggaaaaa aaacattaaa
     7861 tttttgcagg aaattgaggt tttttagctc actgaccgct cttagactca gataggcgaa
     7921 gggcggtaat tatcgattct gaggataccc aaggcatttt aagtgtcagg aaccccaaca
     7981 agaacaagcc tgttgaaacg caatccattt gtgttacggg aaggttataa tttaaaggct
     8041 taaacgtccc cattttaacc ggtacaatgc ttgcgcttac caaaaggccc ccacggtcgt
     8101 tgggtccgat attgatattt ttgtaggctt ggtgagtgcg atgcgtggtc ttacgctttt
     8161 caaattttag tgtcggctta attctcagaa aaacagatgc cagcgccatg gcttcgtttt
     8221 gggatagttt catgagaatg ctgccctctg aattccatcc atattcccta ttgtcagacg
     8281 gaggagcaaa ctggagagac aggtaatact tttcagtctc agttacaaac gccgaattat
     8341 cagcagagcg acttttgtcg agatttactt cgaatgccat cgcgacagat tggctgaata
     8401 ctctgacccg ttgaggcttc gcaccagttg ctgacagatc gctcattgtc atactctctt
     8461 cttaaagaac catgtaccgg acataacttc attatccttc gtgtgcgtca aatccgaaat
     8521 aacaaaacca ccttttcctt ccgaaattat catggtgtaa gagctcgtat gcacaaggcg
     8581 ggaatttttg acttgcagac ccgtacactt aactgcaatt tcattacccg gaactctctc
     8641 aacaaaagta acttttgatg caacgccatt gatatgtacg cttccgttat ctgctatctg
     8701 gatgttcaac gaaggtgtgg attctttatc acaaggttgg aaaacagaag tggttttaag
     8761 aactttgcca gtaaaatcgt acaagtcagg cttcgacggt tttgcgttaa agatcgagta
     8821 agaccccgcc actgttaaac caaccattaa aagggggatt acctttttaa aattaacttt
     8881 catcctccag ttcctctcca atcaagtgta aagcacttac gcacataagc ctgagttgat
     8941 acagcaaatt gtcggcggtg atccccccaa caaatgtcac tgtacggcgt atcgaaacgc
     9001 ctccaggctc gcattgaata tggccaatac ctgcatcgcg aaggaaatca ttctcggcgc
     9061 ttaccaggtc tgcttcagca ggcggaatga ccacttcgat gagtattgta tcaattggag
     9121 cccactccca gtcgtactta agcgatgaaa ttggggcaat ggaaacatga aattcatcaa
     9181 tattaaaaat gactggtttt ttgcctgttg atttaggctg aatgaaggga ttctcgacca
     9241 ggacaactgg gaattcagaa atagacagaa cttcgattaa ctgatctcga cgtaaaccgt
     9301 gtattagtga gttgcgtgat aacattcagt catcctttat tataataatc agatagttac
     9361 acatgatccc gctagttcct taccatcttc cc
//
LOCUS       DAFNBJ010000178         9350 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000178 DAFNBJ010000000
VERSION     DAFNBJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.178"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            178..1272
                     /gene="ddlA"
                     /locus_tag="L2X49_004178"
     CDS             178..1272
                     /gene="ddlA"
                     /locus_tag="L2X49_004178"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBN4799179.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1296..1508)
                     /locus_tag="L2X49_004179"
     CDS             complement(1296..1508)
                     /locus_tag="L2X49_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBN4799180.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1768..2076
                     /locus_tag="L2X49_004180"
     CDS             1768..2076
                     /locus_tag="L2X49_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBN4799181.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2135..3229)
                     /gene="yaiW"
                     /locus_tag="L2X49_004181"
     CDS             complement(2135..3229)
                     /gene="yaiW"
                     /locus_tag="L2X49_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBN4799182.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVGSVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3242..4462)
                     /gene="sbmA"
                     /locus_tag="L2X49_004182"
     CDS             complement(3242..4462)
                     /gene="sbmA"
                     /locus_tag="L2X49_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBN4799183.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4814..5971
                     /gene="ampH"
                     /locus_tag="L2X49_004183"
     CDS             4814..5971
                     /gene="ampH"
                     /locus_tag="L2X49_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBN4799184.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKQLVIPAS"
     gene            complement(5972..6595)
                     /gene="iprA"
                     /locus_tag="L2X49_004184"
     CDS             complement(5972..6595)
                     /gene="iprA"
                     /locus_tag="L2X49_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HBN4799185.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6683..>9350)
                     /locus_tag="L2X49_004185"
     CDS             complement(6683..>9350)
                     /locus_tag="L2X49_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBN4799186.1"
                     /translation="GTISTYYLNHDYASGCVNQVDISNSVIHGSITSELPFGYYSITP
                     EGYNGYTFNNGTTTDLDYNWYDGDVFTLNVSNSTIDDDYEALYFTDTHLDGDKSKSTN
                     ETFYTALGVAVNLDVESNINITNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNS
                     TVTSGSQTPLEESDGFFGKSAEPSDYAGNGGQNDVALSFTDNAGSNYSMKNNVNFDHS
                     TLLGDVEFTSHWNNDGVFFYSTGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKW
                     VGSANMSAEVIAPADMYDVAPNSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNSSE
                     WNTVNSSVIDTLAVNNGSQVNVTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDSY
                     STVNLTESTGWNNYSNLYTNTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHN
                     GNVISGVFDINSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNG
                     TELDNSSVDNVVAATGNYKVRIDNATGAGTIADYKDKELIRVNDANTNATFSAANKAD
                     LGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGL
                     ADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGNNAKWIVGAAAGFAKGD
                     MNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNS
                     DAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELG
                     VDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSF
                     TKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2401 a         2327 c         2223 g         2399 t
ORIGIN      
        1 gcttcccgtt catcattttt gtgatcttgc gcacatcttt gagataacgc actttcttca
       61 ccagtacgtt gcacgctttg tcagcagaag attgcaacaa tctttcagaa ggccgcgaat
      121 cccccgtttt gtcgtggtaa tgtatgcact ttgtgttaga cgggataggt ttttaagatg
      181 gaaaaactgc gggtaggaat cgtttttggt ggtaaatcag cggaacatga agtgtctctg
      241 caatcggcaa aaaacattgt cgatgccatt gataaaagtc gcttcgacgt tgtgctgctg
      301 ggcattgata aacaagggca atggcacgtc agcgatgcca gcaattatct gctaaatgca
      361 gacgatcctg cccatattgc gttgcgccct tcggcgacca gccttgcgca ggtgccaggt
      421 aaacatgagc atcagcttat cgacgcgcaa aacggtcagc cgttgccgac ggtggatgtc
      481 attttcccga ttgtccacgg tacgctgggc gaagatggtt ccttgcaggg aatgctgcgg
      541 gtcgccaatt taccgtttgt aggttctgat gttctggctt cagcagcctg tatggataaa
      601 gatgtcacca aacgtctgct acgcgatgcc gggctgaaca ttgcgccatt tattaccctg
      661 acgcgcgcta atcgtcacaa catcagtttt gccgaagtgg agtctaaact ggggttacca
      721 ctgtttgtaa aaccggctaa tcagggctct tctgttggtg tcagcaaagt aaccagtgaa
      781 gaacagtacg caattgccgt cgatctggcg ttcgagttcg atcataaagt gatcgttgag
      841 caagggatca aaggtcgtga gatcgaatgc gcagttctgg gcaacgacaa tccgcaagcc
      901 agcacctgtg gcgagatcgt actcaccagc gatttctatg cctacgacac caagtacatt
      961 gacgaagatg gcgcgaaagt ggtagttccg gcagccattg caccagagat caacgataag
     1021 atccgggcga ttgccgttca ggcttatcag acgttgggat gcgcaggcat ggcgcgtgta
     1081 gacgtgtttt taaccccaga gaacgaagtg gtgatcaacg agatcaacac actgcctggc
     1141 ttcaccaaca tcagtatgta tccgaagctg tggcaagcca gcggtctggg ttacaccgat
     1201 ctgatcacac gtttgattga gctggcgctg gagcgtcacg ctgcggataa cgcattgaaa
     1261 accacaatgt aatattttaa cgcccgataa tattcttatt gggcgttttc agtttcttca
     1321 tcgcgacgac gaataatcaa ccccgccagc cagaaactaa tcacccacgt caccagaccg
     1381 acggcatagg tctgccaacc ttttgcttca aatcccagta accccaccac gccattaaga
     1441 atgaatataa ggccaatggc gaacgcatag tagtgccagt cacggcggat ttttacaggc
     1501 agattcatgg cagctccgtt aacgaaaaac aacatccttg catattctgt aacaaccgtc
     1561 tgtggtgtag acaacacttt cgcaattgtt aataaattta acttaaagtt gctgttttgt
     1621 gactcgcaac gaagattcac gcttcgggaa tattcccaac atcaatttac catgattctg
     1681 atattgacaa tctaaatgac ctgtcagact tgccataagc cgctggtatc acaccagaag
     1741 agaagcagat gacttccgga ggtttttatg gctgatttca ccctgtcaaa atcgctgttt
     1801 agcggaaaat atcgcaatgc ctcttcaacg cctggcaaca ttgcctatgc gttgtttgtg
     1861 ctgttttgct tttgggctgg ggcgcaattg ctgaacctgt tagtgcatgc gcccggcgtc
     1921 tatgagcgtt taatgcaagt ccaggaaaca ggtcgcccac gggtggaaat tggtttaggt
     1981 gtcggcacca ttttcgggct gatcccgttt ttagtaggct gcctcatttt tgcagtggtg
     2041 gcgctatggc tgcactggcg acatcgccgc cagtaagttt tacgttgtca gatgcggcct
     2101 gaaacgcctt atctggcatg acattcctgt ttgatcactg tttcatacaa cgcgcccgcc
     2161 gttcgtctac gcgcttcgca aaccaggccg tagtcaggtt gcgggtgatt ttcgggcttt
     2221 ccagttgaat gccaggcaac atttcgcggg gtagtgattt accggttttc gcttcggcaa
     2281 gttgataaac tttcttgtac agcgccgtct cctcaaaaga gaagctgtca cctttctcta
     2341 gctgacgacg aatttcgctg tcgctcatcc ccagttttgc cgccagtttg cgcgttgcca
     2401 gttctgtttt cccgggttct ttactgtcgt aacgaattaa atcaccgtcc agtgccagct
     2461 tcacgccgct ggccttactg accgcgttct gaaacgcggc attacggctg gcgtaccagc
     2521 cagcgttaaa atcagcaaaa cggtatatcg gtgcactata actggcggga taattcagta
     2581 aatggtaagt accaaaccac aacccgccgc ggcgactaaa gacttcctga cgaactgtac
     2641 cgtccatttt ccacggatac cctttggtat gctgttcggc aaaagcaatg ctgacctgca
     2701 tcggcccacc ggtgcgcacc ggattaagcg aaccaaacag cgtctgcccc attggcacca
     2761 tgctgatcag atcgtcaaaa atcgcgctca attgcttttc tgtacgcacc gaatctaacc
     2821 gttcgctata acttttgccg tttggcgatt tgatttttag cgcggtatga accagaaaag
     2881 gaggaatgtg catccgttca gcacgacggt caatctcttg ccaggcgatc ttgcttaagc
     2941 ccggaacggc cggatctgcc tgataatttg actcctgctg cgccaccgcc agcaccgagc
     3001 agacgttttc cagcgtcggt gccagcccct gactttcaaa ggtggtggcg agatctttcg
     3061 cccaggcatc gcggtctttc acgctcgcag gcatcttctg ccgcacgacg ctccctacat
     3121 ctatcgcttt ttcgcccttt ttcaacggtt gcggagcctg actactacac cccgctaaaa
     3181 ccagaaccgc caacaatgac agcgaagata gaggattgac gcgcgacatt acttctcctt
     3241 tttagctcaa ggtatgggtt acttcctgaa ttttgtcacc atccagctca tgttcaaagc
     3301 tgcgcagacg tttgtagata gacatcaact caaccagtgt ggtccatgag ttaatcaggt
     3361 actggaaagc accgcgaacc tgaccaaaaa cgttggtaat ctgcgtcatc aggccgagcg
     3421 taatcgtacc ggcaacaatt gacggaaaca gcaagaacaa accgaaaacg ttatcgacct
     3481 gcaaatagag aatgcgggcg atgttgaaat acatatagtg aaaatagagg cgaaaatagt
     3541 ttttccgtac ggcgctaaac agctcgcgta ccgtaggcgg cgtcgcgcgc gtggcatcat
     3601 cttcaccata aaccagctct ttacggtagg cagcctctac acgctggttt ttaaactcca
     3661 gccccggcag tttgatccct accactgcca gcaatccggt ccccatcagc gaccagacga
     3721 tagcggcaat caccagacca tacggaatgt gcccgataat cggcagctcc ggcacatgcg
     3781 cggagagcgt taccagtacc ggcaggaagg cgattaacgt catgatggcg ttgataaaac
     3841 tgacgcccat attctccagc gttgaagcaa aacgcatggt gtcttcctgc acacgctgtg
     3901 cggcaccttc gatatgacgc agttgttgcc agttcgccat gtaatattcg ttcatcgccg
     3961 tacgccagcg gaacacgtag tgactgacaa agaagttatt cagcacactg atcactaccg
     4021 cgatcagcgc aatccccaga aagacgccca cttcgcggta aaattgttcg atggtgactt
     4081 tatgcggcga acttagcgcg gtttgaatca gatcatagaa cggcgcatac caggcgttga
     4141 cggcaacccc gacttccacc aaaaaccagg tgacgaagat aatcagcgca gtaccgagta
     4201 ttgaccagta ttgccaacga tgcgggctgt agataaacca gaacaatgca aaaagtccta
     4261 cgcaaacaat gtagtaagcg taaaaaatca ggaaatccaa cgaccagaaa cgcgcggcgc
     4321 taatcgggat ctgcccggaa gcgccggtga tacgcgccac ccagtcaccc ccaccggctt
     4381 gccagaagat aacggcaatc aatgcccaaa caaaggccga gagaaaaaac gttcccggct
     4441 ttgggaaaaa agacttaaac atatgcactc ctgctaactt cttatcgttt taacaactgc
     4501 tgtgtattaa ccgcatgacc gctacaaagc gtcagccaaa tgaaaccgcc cattgtgacc
     4561 catagcaagg cgcttagttc gccagcctga ccataagaat tgtttcgacg ggtttcacaa
     4621 accaaagatt gtttacataa gcggattaac acgcgcagga tcacaaatgc aggcattggt
     4681 gagcaggaaa aaaatcgtgt ttacgcgccg ttacgttttt aacactatta ttgcactaat
     4741 tcgccctttg caatccatca atgagtagta taaatacgct cagttacctt cattcaattt
     4801 atggacacca ccgttgaaac gtagtctgct tttttctgcc gtgctgtgtg cggcgtcatt
     4861 gacctccgtc catgcggcgc agccgatcac tgaaccggag tttgcctctg atattgtcga
     4921 tcgttatgcc gatcatattt tttacggcag cggtgccacg gggatggcgc tggttgttat
     4981 cgacggtaac cagcgcgtct ttcgcagtta tggcgaaacg cgacctggta ataacgttcg
     5041 cccgcagctg gattcggtcg tgcgtattgc ttccctcacc aagctaatga ccagtgagat
     5101 gctggtgaaa ttgctcgacc aggggaccgt gaagttaaac gatccgctaa gtaaatatgc
     5161 tccgccaggc gcacgcgtgc caacctacaa tgggacgccc attacgctgg tcaatctggc
     5221 aacccatacc agcgccctgc cccgtgaaca gcccggtggc gcggcacatc gtcccgtgtt
     5281 tgtctggcca acgcgcgagc aacgctggaa atacctttct acggcgaagc tgaaagccgc
     5341 gccaggcagt caggcggcgt actctaacct tgcgttcgac ctgctggcag atgcactggc
     5401 gaatgcgtcc ggcaagcctt atacccagtt gtttgaagag cagattaccc gtccattggg
     5461 gatgaaagac accacctaca ccccctcacc ggatcagtgc cgccgtttaa tggttgctga
     5521 gcgcggtgcc agtccgtgca ataacacgct ggcggcaatt ggcagcggcg gcgtctattc
     5581 cacgcctggc gatatgatgc gctggatgca gcagtatttg tcatctgatt tttatcagcg
     5641 cagtaaccag gcagatcgca tgcagacgtt gatttatcag cgcgcgcagt ttaccaaagt
     5701 gattggcatg gatgtccccg gcaaagcgga tgcgcttggt ttaggctggg tatatatggc
     5761 cccgaaagag ggtcgtccgg ggattattca gaagacaggc ggtggtggcg gtttcattac
     5821 ctatatggcg atgatcccgc agaagaatat tggcgcgttt gtggtggtca ctcgttcgcc
     5881 gttgacgcgc tttaaaaata tgagcgatgg catcaatgat ctggtgaccg agctaagcgg
     5941 gaataaacag ttggttatcc ccgcatcctg attaatactc tgaaggcaaa cggttgatag
     6001 cgaccagttt gcctttattc atttcgatat agccgccttt acgcaaagca gcgagaactt
     6061 ctgcgacgac agaacgcgat atgcgtgtac gttgatggat ataattcatc acaccaatac
     6121 gtgatcgcaa ttcttcattc cagtcaatca ttgataataa tgttgcgcgg atttgttcgt
     6181 aggaattatg cccaatgagc tgcacgtcac gtaattccag aattctattt tgccaggcta
     6241 accagtaaaa agcgtctcgc cagagttgat tttgttcaat aagcgtaatg gtttgtttgg
     6301 ctggtagatg atatcccgta caatttcctt ctgatattaa tttgtatggt atatcgtttt
     6361 tcattaaacc atcagccagc cccataatat aaggagcctg ggtaataccg ataagtacgt
     6421 tttcttctct acgcagagag ataactccct ctaaaataac gaaagtatct tcgttattga
     6481 catcactgga aaatataact tgcttttcat tattaaactc gaagcgcgta ccgtatctgg
     6541 acaaacattt atcgagctta ccaaattcct gaagaggttt aactacagat aacatttgcg
     6601 cgtcctttgc agtaatgccc gtcaaatcct tgacgggcat tatttagatt aaattaccag
     6661 aatttcttcg gagtgaagaa tattaccagg tatatttaac acccacgttc gcggaccagt
     6721 cttgatctac gtcaccacca ccgaggtagt tagcatcggt ataggcgcta aagttcttgg
     6781 tgaagctgaa ctgagtaccc agaccaacac gtaccgcaga cccttcagta ccgttatcga
     6841 tggaatcacc gttcacatcg ttatcgttgt tagagtcgtc gtagacgtaa gccagtttga
     6901 agtacggagt cagagcctga tcttcgctgt aggtgaaggt ataacctgca tctacaccca
     6961 gttcataacg catgctgtcg taagactgac cgtcaacttt catgtcgttg ctcagctggt
     7021 agtcatcacc agactggaac agaccagaaa cgctgccgta aggagtcacg taaccagcat
     7081 cacccagttt gaagtcgtaa ccggctttca agccgaagcc ccaagcgtcg gagttggtgc
     7141 taccgtcaac gtaagtaccg ttgctcatgg ttgcagacag gtcgttgttg aagtgagagt
     7201 agctcaagct accatcaaca aagacgttgt tcgcgaagtg agcagaagag tagatgtagg
     7261 cagtctggct gtcttgatct acctgaccag aacggtcatt catgtcacct ttagcgaagc
     7321 ctgcagccgc accgacgatc cacttagcgt tgttaccgtc aactttggta tcaacaccga
     7381 ccatgatgcc gttaacatcc tgatcatagt tgatggtgcc gttgtcgccg ttgaagttac
     7441 caccgaagta gcttacccat gcgccgccgt tatcagccag gccatgacga gagttggtca
     7501 gacgagtacc aacggtgtct tgttccaggt tccagatatt ggtgttcgca gacgggatgc
     7561 tcagcgccat gttagcgtag tcggtcagct ccatctgttg cagaacaacg gtgttaccgc
     7621 gctgttcagc ctgataggtg tatgcaccca ggtcagcttt gttagcagca gagaaggtcg
     7681 cgttggtgtt agcgtcgttt acccgaataa gttctttatc tttgtaatca gcgatagtgc
     7741 cagcgccagt tgcgttgtcg atacgaactt tgtagttacc ggttgcagca acaacgttat
     7801 caacagagct gttatccagt tcagtaccgt tgtctacgtc gccgttacca ttgatagtca
     7861 ggtgaccatc agagttcata gcaacaatac cgtaaccgta gttagacttg gaagtatccc
     7921 acgtacggtc gttcaccaga tcagcgttca gaacgtaatc gctgctgttg atgtcgaaca
     7981 caccagaaat tacgttgcca ttatggtcag caatgttgcc gctggtcagt tccagtttat
     8041 cagtacggaa agcttcagta tcgaactgat caacattcac atccagcaca ccaccgttag
     8101 tcacagtgat agtattggta tacagattag aataattatt ccaaccggta gattcggtta
     8161 aattaacggt actgtagcta tcaacagtca ggtggtctgt agcaaccacg ccatttgcac
     8221 caatattcag tgaagaaccg ttggttaaac caatggtatc tgataccaga gaggaatcag
     8281 taacatttac ctgagagcca ttatttacag ctaatgtatc aattactgaa ctattaacgg
     8341 tattccattc tgaactattg ttcagtgcta cgttaaatac gccgctctgg aatactttgt
     8401 tgtcaataat acggccccaa tcattagctt cgatggtggc accaggggtc aggctgttcg
     8461 gcgcaacgtc atacatatct gccggagcaa ttacttcagc actcatgttt gctgaaccaa
     8521 cccacttact accgttattc agagtaatat tcagttcgtc aacgttctga gcgtcatcta
     8581 cccaaccgcc gttggtatca aggacgccgt cgccgttgct gtcatgaccg gtggaataga
     8641 agaatacgcc atcgttattc cagtgacttg taaattcgac atcacccaac agagttgaat
     8701 ggtcaaagtt tacattgttt ttcatggaat aatttgagcc agcattgtca gtgaatgaca
     8761 gtgctacgtc attctggcca ccattaccag cgtaatcact tggttcagca gatttaccga
     8821 agaagccatc actttcttcc agcggagtct gtgatcctga agttacagtg gaattagaca
     8881 cattgatgtt gttgtcccac tgatgatatt cgctagtata agttgtgttg cttgtattgc
     8941 cttcagacag cgcaatgcca gctacacggg agttgttggt aatattgata ttgctttcaa
     9001 catcaagatt tactgcaaca cccagagcgg tatagaacgt ttcattggtt gacttagatt
     9061 tatcaccatc gagatgtgtg tcagtgaagt aaagagcttc atagtcatca tcaatggtgg
     9121 aattggatac gttcagagtg aatacgtcac catcgtacca attgtagtca agatcagtag
     9181 tagttccgtt attgaaagta tagccattat aaccttcagg cgtaatgctg taataaccaa
     9241 atggcagttc agaagtgatt gacccatgaa ttactgaatt gctgatatct acctggttaa
     9301 cacaaccgct agcgtaatca tgattcaagt aataagtaga aatggtaccg
//
LOCUS       DAFNBJ010000179         9226 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000179 DAFNBJ010000000
VERSION     DAFNBJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.179"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            205..5625
                     /locus_tag="L2X49_004186"
     CDS             205..5625
                     /locus_tag="L2X49_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HBN4799187.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGMGEATIREGGLVTAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGAVNISNNGLINNKEYSLVGVEDGSHGVVNVTDK
                     GHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDGTSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPPDTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGIYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQVQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5834..6100
                     /gene="hipB"
                     /locus_tag="L2X49_004187"
     CDS             5834..6100
                     /gene="hipB"
                     /locus_tag="L2X49_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HBN4799188.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6100..7422
                     /gene="hipA"
                     /locus_tag="L2X49_004188"
     CDS             6100..7422
                     /gene="hipA"
                     /locus_tag="L2X49_004188"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HBN4799189.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7734..7942
                     /locus_tag="L2X49_004189"
                     /pseudo
     CDS             7734..7942
                     /locus_tag="L2X49_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8374..8937
                     /gene="fimA"
                     /locus_tag="L2X49_004190"
     CDS             8374..8937
                     /gene="fimA"
                     /locus_tag="L2X49_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBN4799190.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGPSVAGTANADATFKVQYL"
BASE COUNT         2541 a         1939 c         2445 g         2301 t
ORIGIN      
        1 actcatagtt catgtttaaa taaaataaga actatgcaat ttttaatcta tcaattcgaa
       61 agcattagca aattagccaa attgacacct tcccacctcc aactaacact caatacagac
      121 ctctgacaat ttaataagtt tcatttttaa tgtctgacga ctcatctctt tcgtggctgt
      181 attgtttgta tatggagcgt ttatatgaat agaatctatc gcgtgatatg gaattgcact
      241 ctacaggtat ttcaggcctg ctcggaatta actcgcaggg caggtaaaac atcgacggtt
      301 aatttgcgta aatcctctgg actgacaacg aaattcagta gattgacgct gggtgttttg
      361 ctggcactaa gcggttcagc gtctggtgca agtctggaag ttgataatga tcagattacc
      421 aatattgata ctgatgttgc ttatgatgcc tacctggttg gctggtatgg cactggagtg
      481 cttaatattt tggctggcgg taatgcctcc ttaaccacta ttactaccag cgtcattggc
      541 gctaatgagg actcagaggg caccgttaat gttttgggtg gcacctggcg attgtatgat
      601 agcggaaata atgcaaggcc tttaaatgtg ggtcaatccg gaacggggac gctgaatatt
      661 aaacagaagg gtcacgtcga tggaggctat ttaagattag gttcttcgac aggaggcgtc
      721 gggacggtca atgttgaggg agaggactct gttctgacga ccgaattatt cgaaataggg
      781 agttacggta cgggctcatt aaatattacg gataagggtt acgtcacgag ttcaatcgtc
      841 gccattttag gctatcaggc gggcagtaat ggccaggttg tcgttgaaaa gggtggcgag
      901 tggctaataa aaaataacga ttcctcaatt gaatttcaaa ttggtaatca agggatgggg
      961 gaagcgacta ttcgcgaggg ggggctggtt acggctgaaa atacgattat cggtggcaat
     1021 gccaccggtg tcggaaccct gaatgtgcag gatcaagact ctgtcatcac ggtacgcaga
     1081 ctctataatg gatatttcgg taatggcgca gtcaatattt ccaataatgg actaattaat
     1141 aacaaagaat attcattggt gggcgttgaa gacggttccc acggtgtcgt caacgtgacc
     1201 gataaagggc attggagttt cctcggaaca ggcgaagctt tccgctatat ctatatcggt
     1261 gatgctggtg acggtgaact taatgtctcg cgtgaaggta aagtagattc gggaattatc
     1321 actgcgggga tgaaagaaac aggcacaggc aacattactg ttaaggataa gaactccgtt
     1381 atcactaatc tcggaactaa tcttggttat gacggccacg gcgaaatgaa tatcagtaat
     1441 gaggggcttg ttgtcagcaa cggaggaagc tcactcggtt atggagaaaa cggcgtcggg
     1501 aatgtcagca tcaccacggg ggggatgtgg gaggtcaata agaatgtcta taccaccatt
     1561 ggtgttgcgg gcgtcggaaa cctcaatatt agcgatggcg gtaagtttgt atcgcaaaat
     1621 attacttttt tgggcgataa agcaagcggt atcggcacac tgaacctgat ggatgggaca
     1681 tcatcgttcg atactgtggg tatcaatgtc ggtaattttg gtagcggtat cgtaaatgtc
     1741 agtaatggtg ccacccttaa ttcaacgggc tatggattta tcggaggaaa tgcctccggt
     1801 aaagggatag ttaatatttc aacggatagt ctctggaatt taaagacatc atctactaac
     1861 gcccaattgc tacaggtcgg tgtattaggc acgggtgaac tgaatattac caccggaggt
     1921 atagttaaag cgcgtgatac acagatagct ctcaatgaca aaagtaaggg cgacgtgagg
     1981 gtggatgggc agaactctct tcttgaaaca ttcaatatgt acgtagggac atctggtacg
     2041 ggtacgttaa ccctgacgaa tagcggcacg ctgaatgtcg aaggtggaga agtttactta
     2101 ggtgtttttg aacctgctgt aggaacgcta aacattggtg cagctcacgg tgaggcggcg
     2161 gcagatgccg ggtatataac caacgcgacg aaagtagagt ttggttctgg cgaaggtgtt
     2221 tttgtcttta atcatactaa taacagtgat gccggctacc aggtcgatat gctgattacg
     2281 ggtgacgata aagacggaaa agtgatccat gatgcaggcc atacggtgtt caatgcaggg
     2341 aatacttata gcggtaaaac gctggtcaat gacggcctcc tgaccattgc gtctcatacg
     2401 gcagatgggg taacgggcat gggttcgagt gaagtcacca ttgcaagccc cggtacgctc
     2461 gacattctcg catcaacgaa cagtgcagga gattacacgc tgaccaatgc gctcaaaggc
     2521 gatggtttga tgcgagtgca gctgtcatcc tccgacaaga tgtttggctt tacccatgca
     2581 acagggactg aattcgccgg tgttgcccaa ctgaaagaca gtaccttcac tctggaacgc
     2641 gacaacaccg ctgcgcttac tcacgcgatg ttgcagtctg acagtgaaaa taccacatcg
     2701 gtaaaagtgg gagagcaatc cattggtgga ctggccatga atggcggtac gctcattttc
     2761 gatacggata ttcctgctgc gacgcttgca gagggatata tcagcgtcga tacgctggtt
     2821 gtcggcgcgg gtgactacac ctggaaaggc cgtaactatc aggtaaacgg gacgggcgac
     2881 gtgcttatcg acgtgcctaa accgtggaat gatcccatgg cgaataaccc tctgacgacg
     2941 ctcaatttgc tggaacacga cgatagccat gtcggcgttc aactggtgaa ggcgcaaacg
     3001 gttattgggt cgggtggctc attaacgtta cgtgatttac agggcgacga ggtggaagcg
     3061 gacaaaacgt tacacattgc gcaaaacgga acggtggtcg ccgagggtga ttatggattc
     3121 cgcctcacga ccgcaccagg tgatggtttg tacgttaact atgggctgaa agcgctgaac
     3181 atccatggtg ggcaaaagct gacgttagcc gaacatggcg gagcctatgg cgcaacggcc
     3241 gatatgtcgg caaaaatcgg tggtgaaggg gatctggcaa tcaatacggt gcgacaggtt
     3301 tcgctttcca acggtcagaa cgactatcag ggggcaacct acgttcagat ggggacatta
     3361 cgtaccgatg cggatggtgc acttggcaac acccgggaac tgaacatcag caacgcggcc
     3421 atcgtcgatc ttaatggatc gacgcagacg gtagagacat tcaccgggca gatgggttcg
     3481 actgttttgt tcaaagaggg ggcgctgacg gtaaataaag gtgggatcag tcagggtgaa
     3541 ctgacaggtg gcggaaacct gaatgttaca gggggaacgc tggctatcga ggggcttaat
     3601 gcacgctaca atgcgttaac cagcattagc ccaaatgcgg aagtcagcct cgataatact
     3661 caggggttag gcagaggaaa tattgccaat gacggtctgt taacgctaaa aaacgtgact
     3721 ggcgaactgc gtaatagcat aagcgggaag ggtatcgtaa gcgcaaccgc caggacagat
     3781 gtagagctgg atggcgataa tagccgcttt gtggggcaat tcaacattga tacaggcagc
     3841 gcgctcagcg tcaacgagca gaaaaacctg ggtgatgctt ccgttatcaa taatggcctg
     3901 ctcaccatct ccactgagcg tagctgggcg atgacgcaca gtatcagcgg tagcggtgat
     3961 gtgacaaaac tgggtaccgg gatcctgact cttaacaacg attccgcggc gtatcagggt
     4021 acgacggata tcgtgggggg ggaaattgct ttcggttccg actctgccat taatatggca
     4081 agtcaacaca ttaatatcca taacagcggt gtgatgtcgg gaaatgtcac cactgcaggt
     4141 gatgtgaacg ttatgcctgg ggggacactg cgtgtcgcta aaaccacaat cggcggcaac
     4201 ctggagaatg gcggcacggt tcaaatgaat agcgaagggg ggaaaccggg gaatgtactg
     4261 accgttaacg gcaactatac cggaaacaat ggcctgatga cgttcaacgc gacgctgggc
     4321 ggcgataatt cgcccaccga taagatgaac gtgaaaggcg atacccaagg gaacactcgc
     4381 gttcgggttg ataacattgg cggcgtcggt gcacaaacgg tcaacggtat tgaactcatt
     4441 gaggttggcg gtaattctgc aggtaacttc gcgctgacaa ccggaactgt cgaagctggg
     4501 gcttacgtct acacgctggc taaagggaag gggaatgacg agaaaaactg gtatctgacc
     4561 agtaaatggg acggcgtaac gccaccggat acacccgacc ccatcaataa tccccctgtt
     4621 gtggatccgg aaggcccatc agtttatcgc ccggaggccg gaagctatat cagcaacatt
     4681 gccgcagcca actcgctgtt tagccatcgt ttacacgacc gtttgggtga gccgcagtat
     4741 acagattcac tgcattctca ggggtcggca agcagtatgt ggatgcgtca tgtcggaggg
     4801 cacgaacgtt caagggccgg tgacggtcag ctaaatactc aggctaaccg ctatgtattg
     4861 cagctaggcg gcgatttggc gcagtggagt agcaacgcgc aggatcgctg gcatcttggc
     4921 gtgatggcag gctacgccaa tcagcacagt aatactcaga gtaatcgtgt gggttataaa
     4981 tcggatgggc gcatcagcgg gtacagcgct gggatttacg cgacctggta tcagaacgat
     5041 gcgaataaga ccggcgctta tgttgacagc tgggcgctgt ataactggtt tgataacagc
     5101 gtcagttccg ataaccgttc tgctgacgac tatgattctc gcggtgtgac ggcctctgtt
     5161 gagggtgggt atacctttga agcgggaaca tttagcggca gcgaagggac gctgaatacc
     5221 tggtacgtcc agccacaggt gcaaatcacc tggatgggtg tgaaagattc tgaccatacc
     5281 cggaaagacg gaacacgcat tgaaacggaa ggcgacggaa acgtgcaaac gcgacttggg
     5341 gtgaaaacct acctgaacag ccatcaccag cgtgacgatg gtaaacagcg tgagttccag
     5401 ccttacattg aagcgaactg gatcaacaat agcaaagtct acgccgtgaa gatgaatggt
     5461 caaaccgtaa gccgtgatgg tgcgcgtaat ctcggtgaag tacgtaccgg ggttgaggcg
     5521 aaagtaaata acaaccttag cctgtggggg aatgtcggtg tgcaactagg tgataaaggc
     5581 tatagcgata ctcagggcat gctgggagtg aaatatagct ggtaaaccgt ataagccgca
     5641 tgtcgagatg gcatgcggct taatattgcc gacttcaaag gttttaaagc ctccctttta
     5701 tccgtgatcg cggatatcgc agcgtttatc ccgtacagcg gataagatgt gtttccagat
     5761 tgactttatc ctcactaaag gataaaactg ataatatccc cttaagcgga taaatttgct
     5821 gtggacgtat gacatgatga gctttcagaa gatctatagc ccaacgcaat tagcgaatgc
     5881 aatgaaactg gttcgccagc aaaatggctg gacgcagagc gagctggcga aaaaaattgg
     5941 cattaagcag gcgacaattt ccaatttcga aaacaaccct gacaatacca cgctcacgac
     6001 attttttaag attttacagt cgcttgaact ctcaatgaca ctatgcgaca cgaaaaatgc
     6061 ctcgccagaa tcaacagaac agcaagatct ggagtggtaa tgcctaaact tgtcacttgg
     6121 atgaacaacc agcgggtagg cgagttaacg aagttagcca acggcgcgca cacctttaag
     6181 tatgcaccgg agtggttagc cagccgttat gccagaccgt tgtcactttc gctgccattg
     6241 cagaggggga atatcacctc agatgccgta tttaacttct tcgataacct gttacccgat
     6301 agcccgattg tacgtgaccg gatcgttaaa cgttatcatg ctaaatccag acaaccgttt
     6361 gatttattgt cagaaatagg gcgagacagc gttggtgccg taacgttatt acccgaaaat
     6421 gaaaccataa cgcgcccgat aatggcatgg gaaaagctta ctgaagccag gcttgaagaa
     6481 gtattaacgg cttataaagc agatatccct ctgggcatga ttagagaaga aaatgacttt
     6541 cgcatctcgg ttgctggcgc gcaggagaag acagcgctgc ttagaatagg caatgactgg
     6601 tgcattccga aaggaataac gccgacgacg cacatcatta aattaccgat tggcgaaatc
     6661 aggcagccca atgcgacgct cgatctcagc caaagcgttg ataatgagta ttactgtctg
     6721 ctgctggcga aagaacttgg gttgaatgtt ccggacgcag aaatcattaa agcgggaagg
     6781 gtgcgcgcgt tagcggtcga acgttttgac aggcgttgga atactgagcg aacggtttta
     6841 cttcgcttgc cacaggagga tatgtgtcag acattcggtt taccttcatc tgtgaaatat
     6901 gaatcagatg gaggcccagg catcgcgcgg atcatggcgt ttttgatggg gtccagcgag
     6961 gcgcttcgcg atcgttatga ttttatgaaa ttccaggtct tccagtggtt gattggcgca
     7021 acggatggcc atgcaaaaaa cttctccgta tttattcagg ctggcggcag ttatcgactc
     7081 acgccatttt acgacatcat ttcagcattt ccggtccttg gcggtacggg aatacacatc
     7141 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
     7201 aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag
     7261 atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
     7321 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
     7381 aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt
     7441 tgttaatgag cacgacaatc atgaccgcca acgactggat tcttccttgg tttatactgg
     7501 tgcgcataat gtatattatg ttaaatcatg tctgagtgat gcgaagtgct tttctggtag
     7561 tcgttattcg ttcagggttc aagaacttaa gcctctcctc tttcataatt ggcctgtgca
     7621 tggagaaata gcctggttat tccctgcccc atgctaagag cgcagtgtct gtcgcataga
     7681 cactgcatac gaaaacacca gagggtgctg ttacttattt aaggacggat caagcgggaa
     7741 tgagtccact ccggtatcag aaatggctac gtctcaatga agtcaggcga ccgatgctga
     7801 atgaacatta cgatgtcacc acagcagcct atgctgtcgg ttacgaaagc tatcccattt
     7861 cagtcgggaa tattcgcgga tgtttggaga gtcacccaag agagatatta ccaggttaag
     7921 aaagtctgtc ggtcagcttt aataaaccct gcacttatct gtttagagca attcggtgtt
     7981 agtttcagca agcaaacatt aaccatagct aatgatttat agccatatta accattggta
     8041 gcccttgacc tttcctgaca cttcccttgt tcgcaataat attctgttta gtcacggttt
     8101 ataaataaca tgcataatga aatcgtcatg ttatttagcg acataaaatg tttttgcaaa
     8161 tactctcttc aacaagtgca caataaactt gttgtgatta ggtgtccttg tgatttaaag
     8221 atattatcgt tcttcgtgaa atgtaataac cctggaaact atattctcag ggcaacaacc
     8281 aatcaagaat gattcttgtt tttaagcaat aacagaatga cttaattgtt aactctcttt
     8341 attcagctac ttaaatataa attttggaga attatgaaac tcaaacatgt tggtatgatt
     8401 gtcgtttctg tgttggcgat gtcgtctgct gcggtaagcg cagccgaggg tgatgaatca
     8461 gtaacgacca ctgttaatgg cggtgttatt cattttaaag gtgaagtggt aaatgccgct
     8521 tgtgcaattg attcagaatc aatgaaccaa acggttgagc tgggtcaggt tcgttcttct
     8581 cgcctggcta aagcgggtga cctcagctcc gccgttggct tcaatatcaa gctgaatgat
     8641 tgtgatacca atgtttccag taatgcagct gttgcattcc tgggtactac tgtcaccagt
     8701 aatgacgata cgttagcgct gcagagttca gcggcaggct ctgcccaaaa tgtcggtatt
     8761 caaattttgg accgtacggg tgaggtatta atacttgatg gggccacttt tagtgctaaa
     8821 accgacttga ttgatggcac gaatatactg ccattccagg ctcgttatat tgctctcggg
     8881 ccgtccgtag ctggtactgc aaacgctgat gcgaccttca aagttcaata tctataattc
     8941 aatcttaagg tggctatacg tcatctgaaa ttgaaacagg gtgtgtgtgg catggctacg
     9001 cacacccgag caatttacca atattcatct catttttaat gagaaatggt ttgtatttgg
     9061 agcgctatga aaaatcacat aatatctaaa ctatcaataa acaacagtag ttaaacttac
     9121 tgttatttaa aatatttact ttgtagcaag gttttcctat taaaagagaa ccaacattta
     9181 ttacaaacgc attaagtctg gaaacccggc gactcgccaa tgtatt
//
LOCUS       DAFNBJ010000180         9148 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000180 DAFNBJ010000000
VERSION     DAFNBJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.180"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            69..1847
                     /locus_tag="L2X49_004191"
     CDS             69..1847
                     /locus_tag="L2X49_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HBN4799191.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1887..2366)
                     /gene="xdhC"
                     /locus_tag="L2X49_004192"
     CDS             complement(1887..2366)
                     /gene="xdhC"
                     /locus_tag="L2X49_004192"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBN4799192.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2363..3241)
                     /gene="xdhB"
                     /locus_tag="L2X49_004193"
     CDS             complement(2363..3241)
                     /gene="xdhB"
                     /locus_tag="L2X49_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBN4799193.1"
                     /translation="MFDFASYHRAATLADAINLLVDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3252..5549)
                     /gene="xdhA"
                     /locus_tag="L2X49_004194"
     CDS             complement(3252..5549)
                     /gene="xdhA"
                     /locus_tag="L2X49_004194"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBN4799194.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQIQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5964..6719
                     /locus_tag="L2X49_004195"
     CDS             5964..6719
                     /locus_tag="L2X49_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBN4799195.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSITRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6798..6871
                     /locus_tag="L2X49_004196"
     tRNA            6798..6871
                     /locus_tag="L2X49_004196"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6830..6832,aa:Gly,seq:ccc)
     gene            7105..8715
                     /locus_tag="L2X49_004197"
     CDS             7105..8715
                     /locus_tag="L2X49_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799196.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHVKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPNP
                     IIPLNWSKASMTLMLKEMSSLINDEGNK"
     gene            8782..8961
                     /locus_tag="L2X49_004198"
     CDS             8782..8961
                     /locus_tag="L2X49_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HBN4799197.1"
                     /translation="MINKLLLAYLIGLVVTSTFIFIFSEEKVTYRLFATIITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT         2488 a         2072 c         2239 g         2349 t
ORIGIN      
        1 tcatcatgat ctgtgaacca tcaacgtctt caatgatcga cttttaagta agtctggaag
       61 gtaaagctat ggagcttgct actacgcagt cagtattgat gcaaattcaa ccgacaattc
      121 agcgttttgc cagaatgctt gccagcgttt tgcagcttga ggttgagatc gttgatgaaa
      181 acttgtgtcg cgtcgccgga acgggcgcgt atgggaagtt tcttggtcgc cagttgagcg
      241 gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt gtgacacagt
      301 ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga aaattgccgc gaaaaagcat
      361 ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt ttgattgccg
      421 ttacccacga gcaacaagag catatcagtg ataatttacg cgaattttct gattatgttc
      481 gccatatatc caccattttt gtttcgaaac ttctggagga tcaggggcca ggagataaca
      541 tcagtaaaat attcgcgacc atgatcgata atatggatca gggcgtatta gttgttgatg
      601 atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa aacacttggt gttgtacaaa
      661 ataatattat tgggaaacct atccgtttca gaccattaac atttgagagt aattttactc
      721 atggacatat gcagcatatt gtttcgtggg acgataaaag tgaattaatc attggtcaat
      781 tgcataacat tcagggccga caattatttt taatggcatt tcaccaatcg cataccagtt
      841 tttctgtagc aaatgcacct gatgaaccac atattgaaca attggttggc gagtgccgtg
      901 ttatgcggca attaaaacga ctcattagcc gtattgcacc cagcccatcc agcgttatgg
      961 tggttggtga aagcggcacg ggtaaagaag ttgtcgcccg tgcaatccat aagttgagcg
     1021 gaagacggaa taaacccttt attgctatca actgtgccgc gattccggag cagcttctgg
     1081 agagcgaact gttcggttat gttaaaggcg catttactgg cgcttctgcc aacggtaaaa
     1141 cagggttgat tcaggcggcg aatacgggca cgctgtttct cgatgaaata ggtgatatgc
     1201 cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc acgtgaaatt ctgccgattg
     1261 gtgccagtag cccaatacaa gtcgacattc gcatcatttc tgcaactaac cagaatttgg
     1321 cccagttcat tgccgaaggt aaattccgcg aagatctctt ctaccgactt aatgttatcc
     1381 cgataactct gccaccgctg cgtgaacgtc aggaagatat tgaactactg gtgcattact
     1441 ttttacatct gcatacccgt cgtctgggat cggtttatcc tggcattgct cccgatgtcg
     1501 tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc aatttgatgg
     1561 aatatctggt taacgttgtt ccttcaggtg aagttatcga cagcacgcta ttgccgccaa
     1621 atctgctgaa taatggcaca acggagcaaa gtgatgtaac agaggtcact gaggcacacc
     1681 tgtcactcga tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa atgatccgcg
     1741 aggcgctttc acgtcataac agcaagaagc aagttgctga tgaactgggc atcggcattg
     1801 ctacgctcta tcgcaagatt aagaaatatg agttgttaaa cacataacct cagtgaatcc
     1861 ggcctgaatt caggccggat atccttttac ttcgttttct cgcaatccag aaccgtattt
     1921 acaatcatct gataccccgt gcagcgacaa agatttcccg ccagtccgcg acgaatttcc
     1981 gtaatggtta atggcttctc gcgtggtttc gccagcattg ccgtggtagc cataatcagg
     2041 ccaggcgtac aaaacccgca ctgcaccgcg ccggatttcg cataagcctg ctgaacatga
     2101 gaaagttttc cgcctttcgc ttcaccttca agcgtgcgga tctcttttcc ttcagcccag
     2161 gcggcaaggt ataagcaact gtctattgct gtgccgtcga ccagcaccgt acaggcacca
     2221 cattcaccca cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg gagtaattcc
     2281 gagagcggcg tacctggtgc ggcatgaagc tgaaaaggca tcccgttaat ggtgcattcg
     2341 atggtaattg tttcgctgtg attcattgca attttccccc cgccgcggcg acggcttcgc
     2401 taatcacttt tttggtcatc gtctggatga gatgcagacg aaactcttta ctggcccgcc
     2461 atgaagaacg cggggcgaca tcttgcagga cagattcgct gatagcttcc agcgtttgca
     2521 ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc ggcgttggcg
     2581 cggcaacacc aaatgccagg cgtaattcgc tgaaattgcc gttatccagt cggcaatgtg
     2641 cggcacagcc aatcgttgaa atatccattg cgtcgcgcat ggcatattta aaatgcgcgc
     2701 tgcccacgtg ttctttcggc tggggcggaa aatgaaaggc gacaaggatt tcgtcatgct
     2761 caagagacac tttgcctggc ccagtgtgaa agccattaat cgggacgaaa cgaacaccgc
     2821 gtggggagtg gatctccagc tttgcgtcat aaattagcgt tggcgtggca gaatctgcgc
     2881 tggtggcacc gttgcaaata tttccaccgt aggtagcgac gttacggatc tgcggtccgg
     2941 caatggatga ggccgcagca cataacgccg ggagatgacg ttgagttaca ggatcttcaa
     3001 tgagctgggt aaatgtcgtt gcagagccga tacgtagtga gccatcttcc gccagcgtaa
     3061 ttccccgcag ctccgccaga ttatgaatat caacaatatg gcgataacgg tcattgtgat
     3121 ggtggagctg aatcagtacg tcagtgccac cggcgagcag tttggcctgc gggttgtcaa
     3181 ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca aaatcaaaca
     3241 tgatgttatc ctcaaatcaa tcctgccaga tggaactctt catataaccg ttttggcgtc
     3301 agcggcagtg tattgattgc aacaccggta gccatcttca ccgcgttacg aatagctgcg
     3361 gcaacaggaa ttattggtgg ctcacccagt gacttatgtc cgtatgcgga ttgcggctca
     3421 ttgatttcga cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg cattttgtaa
     3481 tccagcagat tggggttacg gaccacgccg cttttcgcat cgatgatcat ctcttcaaat
     3541 agcgcccagc caatgcccat tcccattccg ccgtgtacct gaccttctgc cagcagcgga
     3601 tttagaatat gccccgaatc atgaacgttg aggatgcggt tgatggtgac tttgcacagt
     3661 gcaatatcga ccgtcagatc aacaaaggta cagccgaacg ccggtgggtt agtggtggtt
     3721 ttgatggagc tttcagcaga gagctgcccg ccgcgttcag ggtggtagaa agcgtccatc
     3781 gccaaatctt ttaacgacat taacggctct tccggtcgtt caaccagcac gatatggcct
     3841 tttatcaggg tcagattcat cgctgactga tgtagcatga ctgcggcgtg agcgatgatt
     3901 ttctctttta ataacagtgc cgcactgcgc agcgcaggcg cggcaacata gctctggcgc
     3961 gaggcaaatg cgccgggatc gaacggcgta acgtcggtat cttgtgttga aataacgcga
     4021 acgtcgctga ccggaacccc cacggtttct gccaccattt gcgagaagac ggtgtcggca
     4081 ccctgaccga tttccgtcgc gccgctttgc acgttgatgg ttccatcctg attcatcaga
     4141 aggcgcgcgc ctgctatttc tacgccgaca ggccaggtat tagaggtgta gctaaaacag
     4201 gcgacgccaa cgccgcggcg taaattgcct tgctggttct ggcattctgc acggcgtttt
     4261 tcccattcaa agattttccg gcctttttca agacactccg gcaaccctgc gctgtaaata
     4321 cgtttgcccg tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa acgaatttca
     4381 acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca tagactcaac ggcaaatacg
     4441 acttgtggcg cgccataacc acgcatcgca ccagccgagg ggaggttggt atagcaggtc
     4501 tttgaactgt aagcgtaggc acaacgagga taaaggtaag cgactttatt cccccccgca
     4561 gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt tagacagaac atccagacta
     4621 taacctttca atgttccgtc gcggttcacg cccatttgcc cgtcaatagt aaaagcgtgg
     4681 cgggtacggg ttgcgaggaa acactcttca cggctaaggg aaactttcac cggaatgccg
     4741 ccaagcttgc tggtcaggaa tgccgccatt ggctcttcca gtacatcctg tttattacca
     4801 aaaccgccac cgacaaatgg tttgatgact cgtacgcatg accagggaat atccagcgcc
     4861 tgaccaacca cgcggcgaac aatgtgcggg atctgggtgc tggaaacgat ggtaattcgc
     4921 gagtcatcct ccatccacgc cagcgatgtt acgctttcca tgtgacaatg ttgaataacg
     4981 ggggtctgat agtgcccctg tatctggtag tcggcggcat cgattgtttg ttggacattg
     5041 cccgtcgaca tcgtgctttg tttcagtaaa ttgccgccgt tatggattgg tgcagcgtcc
     5101 tctgccagcg ccgcttctgg cgtggtgata acgggtaatt cttgccactc aatgctgacc
     5161 aattgcgccg ctttttctgc cgtgagttca tcgcgggcca cgacgatggc aacggcgtca
     5221 ccatgatgac gaacatggcg agttagcagt gcgcgatcgg cggtatcgcg cttgttttcg
     5281 tcaagtgtcc atgcatgtcc agctgtagcg aatgggatat caggcacatc ttcccaggta
     5341 aaaatcgcca gtacgcccgg taaacttctg gcttgttcat cattaatact tacggcataa
     5401 ccatgtgcga tagggctacg tacatatttc gcataacaca tgcccgccat aacataatcg
     5461 tcagtatatc gtgcccgccc ggtgacctta gcaatggcat cgacgcgcat gcatgattca
     5521 cccgtagcgg ttgcttcccg cgcttccata gaaatcccct cgatttgaac atttggttct
     5581 tatggcaaag atttagtcta agcaggcaag atttacgcca gaaacgacga taagcgttag
     5641 gggaggaagg aagtgtgatg acgttaaaaa aacacagata tgatacgaaa caggactctg
     5701 aatagggaca gtgatacgta tcaatatgag tggcgataaa atcaatgtga taaatcagga
     5761 ttattacgca tcaggatggg aaatagggta aaaagtcagg aacttcgata cctctacagc
     5821 gaatattttg ctgatgatac ttccctttgc caaatgaaat taacctcttt ctctattacc
     5881 acgtttttcc agaagcaaga gattgggtat caagaggctg gctgctatga tatggcgtct
     5941 tgttttttaa gcgaggaaag attttgagtg cgggacgcct gaataaaaaa tctctgggta
     6001 tcgtgatgtt gttatcggtt ggactgcttt tggcgggctg ttcgggtagc aaatcatccg
     6061 atacaggaac gtattccggc tccgtttaca ccgtgaaacg gggggatacg ctatatcgta
     6121 tttcgcgcac cacgggaacc agcgtaaaag aactggcgcg actgaacggc atttcccccc
     6181 cttacaccat tgaagttggt cagaaactaa aactgggtgg ggcgaaaagt agcagtatta
     6241 cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc ggttacaccg tcatcagcgg
     6301 taccgaaatc atcctggccg ccagtagggc aacgttgttg gttatggcca acgacaggga
     6361 aagttatcat gccgtattcg acagcagatg gcggcaataa agggattgat atctcagctc
     6421 cacggggtac acctatttac gccgcgggtg caggaaaggt ggtgtatgtg ggcaaccagc
     6481 tgcgtggcta cggtaatctc atcatgatta aacacagtga agattacatt acggcttacg
     6541 cccataatga cacgatgctg gtaaataatg ggcaaagcgt gaaggctggg caaaaaatcg
     6601 ccactatggg gagcacggat gcggcatctg ttcgcctgca tttccagatt cgttaccgtg
     6661 caacggcaat tgatccgcta cgttacttgc cgccgcaggg cagcaagcca aaatgctgat
     6721 ggcgaattaa tcagcagtca gcagcgcggc acttgctaag gagagcgtaa ggtttataat
     6781 gccttacgca tctcgaagcg ggcgtagttc aatggtagaa cgagagcttc ccaagctcta
     6841 tacgagggtt cgattccctt cgcccgctcc aatttatctt cacctcatca atctgccatt
     6901 tttgctgtat ctgattttct atattatctt aattcaatcg cttcagactt ttcgcaatcg
     6961 ccgtaatatt tccccatttt gttgtaaata aaaagcgttt acgcaatgca atccttttgt
     7021 aacaacccag gtaaaacacc ttattttcaa gttgttaaat aagtggttat ctacgctttt
     7081 aacaaacgat tgatggaata ttttatgaaa gggaaatctg cattaactct tctgctcgct
     7141 ggtatatttt cgtgcggcac atgccaggcg acaggggcag aagtgacgag tgaatctgta
     7201 tttaacattc ttaattccac gggggcagca acagataaaa gttatttatc gttaaatcca
     7261 gataagtacc ctaattatcg tcttctgatt cactcagcca aattaaaaaa tgaaataaag
     7321 agccactata ccaaagacga aattcaaggg ttgttaacat taacagaaaa cacacgaaag
     7381 ttaacgttga cggagaaacc ctggggaaca tttattcttg cttctacttt cgaagatgac
     7441 aaaacagcgg cagaaaccca ctatgatgct gtctggctga gggatagttt atggggctat
     7501 atggcattag tatcagatca gggaaatagt gtagccgcaa aaaaggttct gctcaccctg
     7561 tgggattata tgtctacgcc cgaccagatt aagcggatgc aggacgtcat tagcaacccc
     7621 aaacgactgg atggagttcc cgggcaaatg aatgctgtgc atatccgatt tgacagcaac
     7681 tcccccgtga tggcagatgt gcaggaagaa ggcaagccgc agctgtggaa ccataaacaa
     7741 aatgatgcgt taggtcttta tcttgatctc cttatacaag caatcgacac tggtacaatt
     7801 aacgctgaag attggcaaaa aggtgaccga ttaaaatcgg ttgcgctgct gattgcttat
     7861 ctggataaag ccaatttcta tgttatggaa gattccggag catgggaaga agatgcccga
     7921 ttaaacacat cttccgttgc actggtgacc tctggactgg aacgtttgtc gaacttactc
     7981 tcgaagaaag actctgtttt tgtctctgat ttactacgcg aagctaaagc caatgaactg
     8041 gatgaacctc tttcaacaac gaggttgaac cacctcatag ataaaggtta tgaaagaata
     8101 acgttacagc ttgatttagg gggagaatcc ccaggttatc tggaaaaaga taaacattac
     8161 agagaagccg atgcggcttt acttaatgtt atttatcctg caaatcttgc aaagataaac
     8221 accagaagaa aagagcaggt tctaaaaata gttaaaaaac tagcaggtcc gtatggtata
     8281 aaaagatacg agaaggataa ttatcagtcg gcaaattttt ggttcaatga tattaaaact
     8341 gataccgatc aaaattcaca tgtgaaaaga gaaaagagct tcattccatc aacagaagca
     8401 gaatggtttt ttgactcatg gtatgcaaaa tcagcagcaa ttgtttataa agaatcaaga
     8461 aaagaagagt atttgaatga ctcagtgcaa tttatgaata gatcattggc ccaaataaca
     8521 ggcgagaata tgattggtgc aaatggcagg agtgtacctg aaatggcact tcccgaaagc
     8581 tataactata tccacaagtc gggaactttg catgaagcgc caaaccctat cattccgcta
     8641 aattggtcga aagcttcaat gactctcatg ttgaaagaga tgtccagcct tatcaatgat
     8701 gagggaaata aatagttttc ataatgaggc ggtcctggat taaaaggacc gctcaaagag
     8761 aaatcgaaag gggaggtaaa agtgatcaat aaattactgt tagcttacct gataggtctg
     8821 gttgtgacat cgacttttat atttatcttc tcggaagaaa aagtaacata tcggttgttt
     8881 gctacaataa ttacaggttt aacctggccg cttagtctca ttccttccat tataagtctc
     8941 atgattcgta aatccgacta acaagaaaat tatatgatcg attaagaaaa atagaatcga
     9001 acaagagaat aaaatatatg cggggagtaa caaaacactc gcagtgtgtt aatgaagagc
     9061 taacaaatat gatttgtagt gaaaatagtt accaaacaag tgttataaga aatacgaggt
     9121 gtgtttaagt aaaacatatc aacagtta
//
LOCUS       DAFNBJ010000181         9130 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000181 DAFNBJ010000000
VERSION     DAFNBJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.181"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            224..1048
                     /gene="gadX"
                     /locus_tag="L2X49_004199"
     CDS             224..1048
                     /gene="gadX"
                     /locus_tag="L2X49_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBN4799198.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1416..2144
                     /gene="gadW"
                     /locus_tag="L2X49_004200"
     CDS             1416..2144
                     /gene="gadW"
                     /locus_tag="L2X49_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBN4799199.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2507..5620)
                     /locus_tag="L2X49_004201"
     CDS             complement(2507..5620)
                     /locus_tag="L2X49_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBN4799200.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLIKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5645..6802)
                     /gene="mdtE"
                     /locus_tag="L2X49_004202"
     CDS             complement(5645..6802)
                     /gene="mdtE"
                     /locus_tag="L2X49_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HBN4799201.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6862..7140
                     /locus_tag="L2X49_004203"
     CDS             6862..7140
                     /locus_tag="L2X49_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799202.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7141..7668)
                     /gene="gadE"
                     /locus_tag="L2X49_004204"
     CDS             complement(7141..7668)
                     /gene="gadE"
                     /locus_tag="L2X49_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBN4799203.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8467..9039)
                     /gene="hdeD"
                     /locus_tag="L2X49_004205"
     CDS             complement(8467..9039)
                     /gene="hdeD"
                     /locus_tag="L2X49_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBN4799204.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2462 a         2115 c         2104 g         2449 t
ORIGIN      
        1 tgcctttaat gtcacgaaaa gggattattt gcttgctatt aatttcactg cgttcttaaa
       61 cacgtttttg tattataaat ccagtcatcc tgcccgtaga acatcacaca ttatcatcct
      121 gttctcccgc ttcgtttaaa tttatttatc aatcaatttg acttaagagg gcggcgtgct
      181 acattaatta acagtaatat gtttatgtaa tattaagtca actatgcaat cattacatgg
      241 gaattgtcta attgcgtacg caagacataa atatattctc accatggtta atggtgaata
      301 tcgctatttt aatggcggtg acctggtttt tgcggatgca agccaaattc gagtagataa
      361 gtgtgttgaa aattttgtat tagtatcaag ggatacgctt tcattatttc tgccgatgct
      421 caaggaggag gcattaaatc ttcatgcaca taaaaaaatt tcttcattac tcgttcatca
      481 ctgtagcaga gatattcctg tttttcagga agttgcgcaa ctatcgcaga ataagaatct
      541 tcgctatgca gaaatgctac gtaaaagagc attaatcttt gcgttgttat ctgtttttct
      601 tgaggatgag cactttatac cgctgctttt gaacgttttg caaccgaaca tgcgaacacg
      661 agtttgtacg gttatcaata ataatatcgc ccatgagtgg acactagccc gaatcgccag
      721 cgagctgttg atgagtccaa gcctgttaaa gaaaaaattg cgcgaagaag agacatcata
      781 ttcacagttg cttactgagt gtagaatgca acgtgctttg caacttattg ttatacatgg
      841 cttttcaatt aagcgagtcg cagtgtcctg tggatatcac agcgtgtcgt atttcattta
      901 cgtctttcga aattattatg ggatgacgcc cacagagtat caggagcgat cggcgcaggg
      961 attgccgaac cgtgactcgg cggcaagtat tgttgcgcaa ggaaattttt acggcactga
     1021 ccgttctgcg gaaggaataa gattatagaa ttttactcag acataaaaaa aacccggcat
     1081 aagggaccgg gaagaggata gtctgccgtc tccagactaa taaaccgtta taacactccc
     1141 tgttggcacg ggaaactttg tgctctcagt aagttaaata taacttttac tggaaataag
     1201 atcagccatt tttttataaa cataagctat acgcagtgcg aaaatatatt cgtgctgcat
     1261 ttacttatta tcaattaact gttatgcaaa actactttgt ggataaattt tggtcctacc
     1321 aaatctggca gttttttcgc taagaaacag tctggcaaca tttcattagt atactgaaat
     1381 tgaaataatc gcagtatgaa atataaggga taatcatgac tcatgtctgc tcggtgatcc
     1441 tcattcgtcg ttcattcgat atttatcatg aacagcataa aatatcgctg cataacgaga
     1501 gtatcgtgct gctggagaaa aatttggcag acgattttgc gttttgttca ccggatacgc
     1561 gacgactgga tatcgatgag ctgacagttt gccattactt acaaaatatt cgtcagctac
     1621 cacgcaattt agggttacat agcaaagacc gtctattaat taaccagtca ccccccatgc
     1681 cgctggtgac ggcgattttt gatagcttca atgaatccgg ggtaaattca ccgatactga
     1741 gcaatatgct ctacctttcc tgtttatcga tgttttctca taagaaagaa ctgatcccct
     1801 tacttttcaa tagcatcagc actgtttcag gaaaagttga acgccttatt agctttgata
     1861 tcgccaaacg ttggtatctg cgcgatatcg cggaaagaat gtataccagc gagagtctca
     1921 tcaaaaaaaa gttgcaggat gaaaatacct gtttcagtaa aatattactc gcttccagga
     1981 tgtcgatggc cagacgatta ctcgagttac gtcaaattcc tctgcatact attgcagaaa
     2041 aatgtggcta tagcagtacg tcgtacttta taaacacatt tcgacaatat tatggtgtaa
     2101 cgccacatca gtttgcgcaa cattcgccag gtaccttttc ctgacatatt ttgcatttga
     2161 atattggtca ggatctcaca cctgcttcat gtgaaactct tccctgatga tttctgccgg
     2221 gctaccggct agttctcttt cgcagtttgc caggcggtat aggtattttc aggaggaaaa
     2281 gactcggcat gtttgggatt attaagctga caattcatac gattacggga atgtgggtca
     2341 gtattgtgct gttcaaattg atgacgaatg gctggagtgg tttctacttt cagtgctgtg
     2401 tactaagcct ggtattcctg acggttagct ggttgttgag tggtgaatgg ttagcaggaa
     2461 agagtaaggc tgaaccttca cgttcaacct tactctcatt tacacgttac gcttttttaa
     2521 agcgggcaaa gagatgttcc actacaacga aaaagaccgg aacgaagtaa attgccagca
     2581 ctgttgcggc aaacatcccg cccatcacgc cggtacctac cgcgttttgc gcgccagaac
     2641 cggcaccatg actgataacc agcggcagca cgccgagaat aaaggccaga gaggtcatca
     2701 ggattgggcg taaacgcatc cgcgccgctt cgatgattgc ctctatcggc gttttccctt
     2761 ctttctgcat catctcaacg gcaaattcga caatcaggat ggcgttcttg gcggaaagcc
     2821 cgatggtggt cagcaaacca acctggaagt agacgtcatt acttaagccg cgcagatcgg
     2881 tggccagtaa tgcgccaacg acgcctaacg gaacaaccaa catcaccgag aacggaattg
     2941 accagctctc atagagtgcg gcgagggcga ggaacaccac gaccagtgaa atggcataca
     3001 gtgcaggagc ctggtttgag gataacgctt cctgatacga cagtccggtc catgagtagc
     3061 cgacgcctgc cggaagttta gcgaccaggt ctgccataaa tttcatagcg tcaccggtac
     3121 ttttcccggc cgccgcttca cctaaaatct ccattgacgg gatgccgttg tagcgttcca
     3181 atcgcggtga accataggtc cattcggtag acgagtaggc agaaagcggt gccatcgtgc
     3241 cagaggcgtt gcgtacatac cattggttga tgttatccgg caacatacgg aacggcgtgc
     3301 ctgcctggac atacactttt ttcacccgcc cctggttgag gaagtcgttc acgtagctgc
     3361 tgccgaaggc ggtggaaatt gtctggttga tatcagacag cgccacaccc attgcctcag
     3421 ctttcgcggc gttgacgttc actttgaaca tcggcgtatc ttccaggccg ttcgggcgta
     3481 ccccggtgac ctggtcaggc gattgcgctg ccagtgataa cagctcgttt cgtgcctggg
     3541 ttagtttttc gtgccccagg ttaccgttgt ccagcagttc catatcaaaa cctgacgcgg
     3601 tacccagttc agccaccgcg ggtaagttga acgggaagac gacggcttta ttgatactgc
     3661 ttaacgcaat cattgcccgc tgaatgatcg cggtaaccga gttttcctca ccgacacgtt
     3721 cagaccacgg cttgagactg ataaacgcca ggccgttgtt ttgcccctgg ccgctgaagc
     3781 caaagccgcc aacggtaaac accgactgaa cattatcttt ctcttttata agataataat
     3841 ccgtcacctg ttgcagcact ttcgtggtgt taaccatggt ggcaccggaa ggtaactgcg
     3901 cggtggtcat aaataccccc tgatcctctt ctggtaagaa agaggtcggt gtacgcagga
     3961 acagcaccgc catcccggcg caaatcagca ggtagaccac catgtagcga ccggtacaac
     4021 gcaacagcga gcgggtgcta tcggtatagt gctgagttga tttttcaaac agcgtgttga
     4081 agcgtgcgaa cagggcgtta ggtttgtgac cgccttccgg cgcggctttc agaatggtgg
     4141 cgcacagggc aggggtcagg ctcattgcca caaatactga aagcagcatg gaggagatca
     4201 gcgtgatgga gaactggcgg tagatctccc cggttgcgcc gctcataaag gccatcggca
     4261 taaacactgc ggaaagaaca acagcaatac cgaccagcgc acgttggatc tgccccatcg
     4321 atttatgcgt cgcttccttc ggtggtagct tatcttccgc aatgacacgc tcgacgttct
     4381 ccaccaccac gatggcgtca tccaccagta gccctatcgc cagcaccatc ccgaacatag
     4441 ttaacgtgtt gatggtgaaa ccgaccgccg acaagatcgc aaacgtcccg agaataacca
     4501 ccggtacggc aatcgtcggg atgattgtgg cacggaaatt ctgcaaaaac agatacatga
     4561 ccaggaagac gaggatgata gcctcaacca gtgttttaaa aacttcctga atagaaattt
     4621 cgataaacgg cgtggtgtcg taaggataaa ccgtcttcag acttgcgggg aaataggctg
     4681 ataagcggtt cagctcctct ttgactgccc gcgaggtatc cagggcgttt gctccggtag
     4741 ccagtttgat ggcgatcccg gcagcaggtt tgccgttata gcgcgccacg gtggaataat
     4801 cttccgctcc aagttcgacg cgggcgacat cacgcagcag cacttgcgaa ccatcttgct
     4861 gaactttcaa caggattttg ccaaattctt ccggcgtttg cagacgcgtc tgcacaatga
     4921 tcgaggcgtt tagttgctgg tctgccgcct gtggcatgcc acccagttga ccaccggaaa
     4981 tctggttgtt ttgcacctta atctgggaaa taacatcgga aggtaccaga ttgtatttat
     5041 tgagtttttg cgggtccagc cagatacgca tggcgtactc ggaaccaaag agctgtacgc
     5101 taccaacgcc cgcggtacgg cttagcgggt ctttgatatt agacgctaca tagtccgcga
     5161 tatcgtactg gttgaggctg ccgttatcag aaataaacgc cgctaccatc agaatattac
     5221 tgctcgactt atcaacgcta atcccctgct gctgcaccgc ttcaggtaat gaaggcatag
     5281 cgagttgcag tttattttgc acctgaacct gtgcgatatc aggagatgtc ccagtctcga
     5341 aggtcagagt gatagaggca ttacccgccg catcactggt tgaagacatg tacatcaggc
     5401 catcaagccc attcatattt tgctcaatca cctgagtgac cgagtcttct accgtttgcg
     5461 catcggcacc tggataggta gcgctgacgg taatggtcgg tggcgcaatc tgcggatact
     5521 gcgcaaccgg taagttcatg atcgccagac cacctgcaag catcataata atggcaagta
     5581 cccaggcaaa aaccgggcga tcaataaaat agttagccat gaaagtcccc ttaagcctgc
     5641 aacgttattg tttcgattcg gtgctggcgt tttcctggct ggaggaaatt gctcgtgctt
     5701 tgatacccgg acgaatgcgt tgcaaaccgg aaacgatcac ccgatcgcca gcctgcaagc
     5761 cagaggtgac aacccactgg tcgccgatgg ctttgctggc ttcaatttcg cgtagctgca
     5821 caacatcgtc tttatccaga atgagcgccg ttgctttacc ctgggcgttg tgggtgacgc
     5881 cttcctgcgg caccagtaat acattctggc ggctaccttc atccactaat gccgtgacgt
     5941 acatgccagg cagcaagtca ccatttgggt tggggaaaat cgcccgtaac gtcacggagc
     6001 ccgtggtttc atccaccgtc gggtcggaga atttcagcgt gccggtctgg ctgtagcgtt
     6061 taccattttc cagattgagc tgtactggcg tactgccctg aacctgtttg atttgcccac
     6121 tggcgacctc ttctttcatg cgtaagaaat cttgcaccga ctgcgtgaga tcgacataaa
     6181 tcgggtccag acgttgtacg gtaaccagcg aatctgcctg attagcggta acgagtgcgc
     6241 cgacggtcac cgacgatttc ccgctgacgc ccgtaatcgg cgaggtgaca ttcgcgtatt
     6301 gcagattgat cgtcgcctgt tcaacagccg ctttggcgac ggtgacattg gcttctgctt
     6361 cattcaactg ggtgcgcgcg gtgtcgtaat cctgacggct aacgtagttg gtcttcagca
     6421 acgatgcctg gcggttaaag gtgatgcggg cattgctggc ggtagagagc gctttcgcca
     6481 gcgagccttt ggcggagttt agctcggcct gtaaaggtgc aggatcaatc tgatacagcg
     6541 aatcgccctg gttcacttta tcgccttcga taaagttgcg tttaatgata ataccgccca
     6601 cctggggacg tatctcggca acttcataag gaacggttct accgggcaat tcgctcaaca
     6661 cattgaccga accgggggag agtgtgacga caccgacctc aggcgtcatg gcggcggcgt
     6721 tttccgccga tttgtcatcg caggcggtga gcatcgcgcc gcagaataac aacggtatta
     6781 acagctttct tcttctgttc attttagtcc ctgaaaattc ttgagaaata acggttcttt
     6841 aaattgcgcg cgaggagaga tatgagggat ttacagacca gcggcatagt ggggttgtca
     6901 gccagtaagg tgggatacag gtatagtgat cgacatggtg aggtcaacga caaaaaaaat
     6961 gttgccgttc tgccaacagt tcctgccagc attcgggcaa cgaagggttc tactggtgga
     7021 tacacatacc aggggaataa agtcgtttta gcctctcctt tagtaatcac cgggggtaat
     7081 gaaatcagca tctgcatccc tcgtcatgcc agccatcaat ttcagttgct tatgtcctga
     7141 ctaaaaataa gatgtgatac ccagggtgac gatgtcgctc atacgtctta gtttcagctt
     7201 gtacataatg ttgaatttct gaatctttaa tgtttgttgc gaaatattga gcgtcgacgt
     7261 gatattgctt tcatttttat ttttcagaat cagttgcata attttgaact gattatgtga
     7321 aaaacataat tgtgaattct tatggggcaa gtgtttacca taagtaatct caagaagatt
     7381 tttgatactt tctttgcggc taacaaaata aataggcgct ttagctttta gtttactgat
     7441 gtgataaggt gccaaaacaa taattctctc ggcatctaat ttctccagaa atttaatcgc
     7501 ttcttcatca aggatatgat tgtgataggt atcaatgata accttgaagg gtttattgcc
     7561 tacttttttg atctctgaca gggaattgat ttttatcatt tcgtgattat ctttcaactg
     7621 ccaaaagccc tgtaaaagaa aagaatcttt cgtcatgaga aaaatcataa cttgctcctt
     7681 agccgttatc gtttcgaaca tgtcatcctt ttcattattt acatccttgt ccgaatcgtt
     7741 gttcaatata gtaaacgcct ataagaactt tgcccttcgt gaactaatac gtctattaac
     7801 actaatcaat acttcagatt tatccatcac gcactccata tattgatcct aagtaagatt
     7861 cctggttgtt atcagtttgt aaacagtaaa caaaaagtta acgtttgcgg attactgccc
     7921 aagaataagt tatcaataac cagtttgtga tctctgaaga atattactaa agttaaaatg
     7981 taatccgatt taaatatcga gtctccttgt ttcgacttaa gctggcaatt ggattgccag
     8041 ctttctttta acgcattgaa attacctgag taaataacgc attcatgtct tcaggttttt
     8101 atatagcgtc tggagacacg gatatttatg caatgtaatc aatgccagtt gcaaaaaaat
     8161 gaacccggat tacttattta accgcccata gcatcaacgc ttacggaact ctttgttcac
     8221 tttcccactg cacttttaaa acattaaacc tgactacgga aaatatcagc catgataatg
     8281 tttgaaatgg ctaatttgcc atggagtgaa aaaaattaga tgaaattcag taggttgaaa
     8341 taatcactag caggtaatta tttcaatgat agtgcgcaat tgatctacaa cgctgcgtag
     8401 cggtgagagt attaatcgga tcatagtcac atcaagtgac tatgatccgg gtgacaaccg
     8461 ggataattat tgctgcttaa cgaacaaact ggcgaagctg aacaggctgg cggcgctaaa
     8521 tatcagttca attcccacca gtgtggaaac cagcgttaca gacaccatcg gcgttgcacc
     8581 aaggaatatc caggcaatga cgatatccag cacaccaata acgagctgta gccagctgcc
     8641 tttcattgaa cgctgacgat accaactcat caggcgaata acccctgcaa cacagaacaa
     8701 accggcaata aatgccgcaa tggcaaaaat gcccagctcc ggtgcgcgga tgaagaaata
     8761 gccgatcaat aaataggcga ctgcgacgag gaaaccggat aataccggcc agaaattatg
     8821 actgcggttg ctgaataacc cgacaataag cgcaatgccc gagcagatta ataatgcacc
     8881 cactactgtg cttaaaatat cgccagagac gaacgggaaa ctgatacaca gcaacccgac
     8941 gataaacaac agcacggcaa taaactggat tgctctgcga tgttttttaa gcatctccag
     9001 atcaaacttc aaaattgttg ccttatctat atataacata gaaccaccct ataaaattaa
     9061 gaagaaaatc ccctgctatc aatctatgcc aaaaacgcgt ctaagaatgc agtcgattta
     9121 ataaaaattt
//
LOCUS       DAFNBJ010000182         9045 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000182 DAFNBJ010000000
VERSION     DAFNBJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.182"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(121..1305)
                     /locus_tag="L2X49_004206"
     CDS             complement(121..1305)
                     /locus_tag="L2X49_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN4799205.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(1605..1699)
                     /locus_tag="L2X49_004207"
     tRNA            complement(1605..1699)
                     /locus_tag="L2X49_004207"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1663..1665),aa:Sec,seq:tca)
     gene            1992..3374
                     /locus_tag="L2X49_004208"
     CDS             1992..3374
                     /locus_tag="L2X49_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HBN4799206.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3384..5702
                     /gene="yicI"
                     /locus_tag="L2X49_004209"
     CDS             3384..5702
                     /gene="yicI"
                     /locus_tag="L2X49_004209"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBN4799207.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTSKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(5754..7463)
                     /locus_tag="L2X49_004210"
     CDS             complement(5754..7463)
                     /locus_tag="L2X49_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBN4799208.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PITGDGNIQLVASGAIQANATLKPTVNGQLHAVNAEKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(7584..8975)
                     /gene="xanP"
                     /locus_tag="L2X49_004211"
     CDS             complement(7584..8975)
                     /gene="xanP"
                     /locus_tag="L2X49_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBN4799209.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVLSRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
BASE COUNT         2207 a         2242 c         2336 g         2260 t
ORIGIN      
        1 aagttggagt cattaccgaa gacgcaaaaa aaataatata ataacaaaaa tcctccagcc
       61 ttgtaggcca gaggataaaa tacttaattg aggaaaatat cagggaaaag tatttctgat
      121 ttaactttcc cggctggaga gtgaatcaat gtaatcagcg taccattgca tcatttcacg
      181 gcggccatcg agatacaggg catgattata ggtacccctg atggaatttt tatccacatg
      241 agctaactgc atttcaatcc aggcggaatt aaagccttgt tcatgcagaa ttgtactcat
      301 ggtgtgtctg aaaccgtgcc ctgtgagtct tcccttatat ccaagtaatt caattacttg
      361 gttaatgctt tccttactaa ttggtttgcg gggatcattt ctaccgataa agactagggg
      421 atgatgtttt gagattggtt caagctgctt aaatagcata atgacctgct cagagagcgg
      481 cacaatatga ggtcgcttca tcttcattac ttctgccggg atctcccaca attttgtctc
      541 aaaatcgata tcttcccagc gtgcaaaacg cagttcttgt gttcgtacgc cggttaacat
      601 aatgatctga gtagctgtct tagtgattat acttccggta taacccgcca gatcgttgag
      661 gaagtgggga agttcattag cagtaagaaa gggaaaatgt acttttttcg gtgtagctaa
      721 agcactggca agatcggggg ctgggttata gtctgcccgg ccagtaacaa ttgcatagcg
      781 gaacacctca ccacagcgct ggcggacctt gcgcattttc tccagagctc cacgtttttc
      841 cattttacgc agtgcttcca gtaactccat tggtttgatt tcggcaatag ggcgcttgcc
      901 aatgtaggga aaaatgtctt tttcaaaggt atcaatgatt tcatcacgat agcgtaatga
      961 ccaccgatca gcttttgact ggtgccattc gcgagcaata gcctcgaacg tatttttcag
     1021 actcatttgc tgcgcgattt tttcttcctt ttttgcctcc cctggatcgc ctccagcagc
     1081 gaggagtttc ctggcttcac tgcgtttctc gcgagcatcg gctaacgtta catcggggta
     1141 tacgccaata gaaagcatct tttctttacc agcaaagcgg tacttcatgc gccagtattt
     1201 agaaccatta gtgttaatca gtagatacat accgccgcca tcagcaagct tatagggttt
     1261 gtctttgggt tttgcggttt tcactttgat atcggttaat gccatgacat cctccggctt
     1321 ttgatgaatt catcttcctg agggggattt ctgccagtaa ctaacctgat acccccaata
     1381 caccccttgt ataacaaaga tattgttaaa agccgttgtt atgtatcata tttttcctat
     1441 tatgggggtg cttgtatttc aataggaaat aacaccccct aaagtacccc caaaagaaac
     1501 ttgatatggg gtgaagtagg ttgacctcag gatacaaata aatactgata tacaagggat
     1561 tagaagagat ttattgacat tgggagagtt cagaagaggt aaactggcgg aagatcacag
     1621 gagtcgaacc tgcccgggac cgctggcggc cccaactgga tttgaagtcc agccgcctca
     1681 ccggagacga cgatcttccg cgcctcgatt gctacatgga ggcggggcgc attatagcta
     1741 cttccttgag tttctacatc ccccagatca gttttttccg cctaatacac gcagcgtatt
     1801 atcacgaaag cattatagtt catataaaac ccatatttta caatgatata tgagcggaat
     1861 taacgctacc acgatcacat aaaacaagaa tcataaatta ataaccagat atcggaatat
     1921 tcgctctcac agggatggtt tacaaaatgc gttatcggtc tcagcacccc tatagcatca
     1981 aggaaaagca gatgaagagt gaagtgttgt ccgttaaaga gaaaattggt tatggcatgg
     2041 gagacgccgc cagccacatt attttcgata acgtaatgtt atatatgatg ttcttttata
     2101 ccgatatttt tggcattcct gccggttttg tcggaaccat gtttttggtc gctcgtgcac
     2161 tggatgcgat ttccgatcct tgcatggggt tgttggccga tcgaacgcgc tctcgctggg
     2221 gtaaatttcg tccgtgggta ctgtttggcg cactgccatt cgggatcgtc tgtgtactgg
     2281 cctatagcac gccagatctc agtatgaacg gcaaaatgat ctatgcagca attacttaca
     2341 ccctacttac cttactttat accgtcgtca atatccctta ctgcgcattg ggtggtgtaa
     2401 tcaccaatga cccgactcag cgtatctcgc tgcaatcctg gcgttttgtg ctggcgaccg
     2461 cgggaggcat gctttctact gttctgatga tgccactggt taatttaatt ggcggtgata
     2521 ataaaccact cggtttccag ggcggtatcg cggtcctttc cgtggtggca ttcatgatgc
     2581 tggcattttg tttcttcacc actaaagaac gcgttgaagc accacctaca acaacgtcta
     2641 tgcgggaaga tttacgtgat atctggcaaa acgaccagtg gcggattgtc ggtttactaa
     2701 ccattttcaa tatcctggcg gtgtgcgtac gcggtggggc gatgatgtat tacgtcacat
     2761 ggattttggg cacgccggaa gtgtttgtcg cttttctcac cacttattgc gtgggtaacc
     2821 tgattggttc cgcactggca aaacctctga ccgactggaa atgtaaagtc actatcttct
     2881 ggtggacgaa cgccctgctg gcagtgatta gcctcgcgat gttctttgtt cccatgcagg
     2941 ccagcatcac tatgtttgtc ttcatcttcg tgattggtgt gttgcatcaa ctggtgacac
     3001 ctatccagtg ggtaatgatg tccgataccg tcgactacgg cgagtggtgc aatggtaaac
     3061 gcctgaccgg gatcagtttt gctggcacgc tgtttgtgct caaactgggg ttggccttcg
     3121 gcggcgctct tatcggctgg atgctggctt atggcggata tgatgcggca gaaaaagcgc
     3181 agaacagcgc cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt
     3241 atttgctgag cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa
     3301 ccgttatgga acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag
     3361 aagtgcagaa ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg
     3421 gcctcaattt gattcacccg cttcaggtat tcgaggttga acagcagggt aatgaaatgg
     3481 tggtctatgc tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat
     3541 ttacgctgcg ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc
     3601 agggggcgct gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca
     3661 caatcgaaaa cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca
     3721 gcaaaggtga gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc
     3781 aggtgaaaaa taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc
     3841 ggcttgatct tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg
     3901 tgcgcaatgg ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg
     3961 cgtataaaaa tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc
     4021 cccagtgtgt ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg
     4081 agagtgaata tctcgaatac tttgttatcg acggcccgac gtcgaaagcg gtacttgatc
     4141 gttatacccg ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc
     4201 taaccacttc atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta
     4261 tggcggaacg caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct
     4321 tccagtggtg cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc
     4381 gccgcctgaa agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa
     4441 aatcccccgt ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt
     4501 cgctatggca gtgggataaa tggcagccag gtctggcgat ttatgacttt accaatccgg
     4561 atgcttgcaa atggtacgcc gacaaactga aaggtctggt cgcgatgggc gttgattgct
     4621 ttaagaccga ctttggcgaa cgtatcccaa ctgatgttca gtggtttgac ggttccgatc
     4681 cgcagaaaat gcataaccat tatgcgtaca tctacaacga actggtgtgg aacgtgctca
     4741 aggacaccgt tggtgaggaa gaagctgtct tgtttgcccg ctcggcctcc gtcggtgcgc
     4801 agaaattccc ggtacactgg ggtggcgatt gttacgctaa ctacgaatca atggcggaaa
     4861 gcctgcgcgg tggtttgtct attggccttt caggttttgg cttctggagc cacgatatcg
     4921 gcggctttga aaataccgct ccggcacacg tttacaaacg ctggtgcgcg tttggtttgc
     4981 tctccagcca tagccgttta catggcagta aatcttatcg tgtgccgtgg gcctatgatg
     5041 atgagtcctg tgatgtggtg cgcttcttca cgcaactgaa atgccgcatg atgccgtatc
     5101 tgtatcgtga agctgcgcgt gcaaacgcgc ggggtacgcc gatgatgcgg gccatgatga
     5161 tggagttccc ggacgatccg gcttgtgatt accttgaccg tcaatacatg ttaggcgaca
     5221 acgtgatggt tgctccggtg ttcactgaag cgggcgatgt gcagttctac ctgccagaag
     5281 gtcgctggac acacctgtgg cacaacgatg agctcgacgg tagtcgctgg cataaacagc
     5341 agcacagctt cctgagtctg cccatttatg tgcgtgataa caccctactg gcgctgggca
     5401 acaacgatca acgtcccgat tacgcgtggc acgaaggcac ggcattccac ctctttaatc
     5461 tgcaagacgg gcatgaagcc gtctgtgaag tgcccgctgc tgacggttcg gtgatcttta
     5521 ctctaaaagc agcacgtact ggcaacacga ttactgtgac tggtgcgggc gaggcgaaga
     5581 actggacact gtgcctgcgc aatgttgtga aagtaaatgg tctgcaaggc ggttcgcagg
     5641 ctgaaagtga gcaggggctg gtggtgacgc ctcaagggaa tgcgctgaca attacgttgt
     5701 aatctattga agaatgccgg gtacgtctga gtcgtacccg gcgagattga tgtttactgc
     5761 accggctctg tcgatgtaac ttcgctactg gaaacgacgc ccgcattcat ggtttgagtc
     5821 acttgctgct tttcagcatt tacggcgtgg agttgcccgt taacggtagg tttcagcgtg
     5881 gcattggcct gaatcgcacc gctggcaacc agttgaatat tgccatcgcc agttatgggt
     5941 aactcaggcc atccccactg ttgcagaata ttcaccggaa caccgcgtcc gttcagactg
     6001 atatgggtct gacgttgtgg cgtttgggat acgctggcgg tggcttccag aatgcctttt
     6061 tcagtaaatg cactcagttc gctgatattc accgtgctgc tgttggcggt cagcgccagc
     6121 gacgggcgac gaacatcaac acgattgaat gtcgcggcgg cggcattcag attcgccgag
     6181 ccactccaga cgccccattt atgatcggta accagcgtca ggttggcacc gtaaccatcg
     6241 agcgcggtga gctgccacgg gaagtcaggg tcgatatcaa tgatcagatt gcggctggcg
     6301 ctaaatctct tcagttgcag gctgtttaac caaccgggtg tcgtttccat ccacaactgt
     6361 tgccagtttt tcggcaaggt atattccagc ccggcaattg ccgcatcatc aaggatcaac
     6421 gttttcccgt cacgcagcca gttccctgac gttctgacca tacccccttc ccagcggctg
     6481 gtgaactggc gcagcgctac gccctgcggg gaaaattccg cgtttataat cgggtcaaat
     6541 aaatgcagcg aaccataaat gaactcgcta gcgttcatcg acagtttgcc ttcctgtgtc
     6601 tgccagtcat ctttactgaa ggtcatgttg cgcaagctga gatcgaggtc ggtcaccgcc
     6661 cagtccggac cttgcaaacg agcatcgatc acttccaggc gaccaatctg caacgacggg
     6721 acagagcgta atggcgcaaa gaagtcggtt agcgattttt cgctttgtag acgtatatcc
     6781 gccatgcgca ggttttccac ttgccagctg ccgtcggcgt tacgctgcgc gtttccggtt
     6841 aatgtcccgc gggcgatgtc ggcacccagg ttagtcagcg taacgcgatc attatcaata
     6901 ctgccttcaa tcagtacatt ggtggcagga acatcattga gcgaaagcga tccggcacta
     6961 aactgaatct gcgccttcgt acccagcact ttaccggctt ctggtgacca cggaaccacg
     7021 ccgccattta cccgctgcgc gctcagtttc cattcgctat tcgggctatt aaacgccata
     7081 tcacgcagtt gcagacgatc ggctttgaac ggtagcggcg cggtctggtc ggtgagattc
     7141 agcgtcccgt tttccagcag gatggtatcg acatggcgtg gttcggtcag ttgccgactg
     7201 cttagcgcaa tgtcgacact ttttgccacc agggtcgcgg gctgaccatc acgaccaaac
     7261 gtgacgttct ccagcacgat atgagatggc gcggaaaaac ggtgatccat cgccccgaag
     7321 gccagatgat agtcgctatt ctcggaaacc catgcgctga tatgttctgc tccccagcgg
     7381 gtttgcagaa gaaaatagag gccagcgatc accactaaca gagcgatgag aatgtagaga
     7441 agcagcttcc caataaattt catggtcttc catcccgtga aatgcacata agggagttat
     7501 gcacgattta cgcgcaatcc tcaaggcggg aatggtgaaa gaggtgagac tgcggcggta
     7561 attatcttac cgccgccagt gaattactgt ttttctggtg ggaaaatcag attcagcacg
     7621 atggcagtaa taccgcccgc ggcgatcccg gaggagagca ggtttttcag ccattcaggg
     7681 gcaaactgca aaatcagcgg ctgctgagac acgcccagac caaccgccag cgacagcgcg
     7741 ataatcagaa tcgcccgacg gttcagcggc tcacgagaaa cgatacgcac accggaggcg
     7801 gcgatggtgc caaacattac aagcgttgcg ccgcccagaa ccggttctgg aatgtgttgt
     7861 acaaaaccgc tcactgccgg gaacagaccc agcacgatca acatcagcgc gacgacaaaa
     7921 ccgacatagc ggctggcaac accagtcaac tggatcactc cgttgttttg cccgaagcag
     7981 gagttcggga aggtgttaaa caccgccgaa acaaacgagt tcaggccgtt tgccagcacg
     8041 ccgcctttca ggcgtttcat gtacagcgga ccggacactg gctgttcgga aacgtcagag
     8101 gtcgccgtga tatcgccaat ggtttccagc gaagtgatca taaagaccag catcagcggc
     8161 agcagcagac tccattcaat gccaagaccg taatagagcg gcgttggcac cataatcagt
     8221 tcttgcgtca tcggttcgtt gctttctggc aacatgccca taaaccacgc cagcgcatat
     8281 ccggccgcca tcgcaatcac cagtgaggcc acgcgtaagt aagggttacg ttgacggtta
     8341 agcaggataa ttaaggctaa gaccacgcct gccagcagca gatttttcgg tgcgccgaag
     8401 gtgttatcgc tcatggctgc gtaaccgccg ccaatggacg ttaacccaac ctgaattagc
     8461 gacaggccga taatcatcac cacaacgcca gaaaccagcg gcgtaataat gcggcgcgcc
     8521 agatgcagaa cgcgggagag caccatctcg gtgcaacttg ccagcatcaa cgtgccgaac
     8581 aaagccgcca tcatggtagg aacatcagca ccaccggttt tcagcgcggt accgcccata
     8641 atcagcgggg caacaaagtt gaagctggtg ccctgaatag acaacagccc ggagccaacc
     8701 ggaccccagg ccttaatttg aataatcgat gccacaccgg aggcaaacag cgacatacta
     8761 ataatgtgtt gcgtgtcttg tgccggtaaa cccagcgcct ggcagattaa tagcgctggc
     8821 gtgatcaccg caacgaacat cgccagcaga tgctgacagg cggcaaacag ggtttgagga
     8881 agcggcggac gatcttcaag acggtaaatc agttcgctgt tttgagtctg cgcaaccggt
     8941 tgcgcatttt ctgactcgag ggtggaaaca gacatcgaaa acaatcccgt ggtagcaaag
     9001 cgggcatttt agctgaccga aagagaaaag caaacgtttg ccaca
//
LOCUS       DAFNBJ010000183         8892 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000183 DAFNBJ010000000
VERSION     DAFNBJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.183"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            334..687
                     /gene="arsR"
                     /locus_tag="L2X49_004212"
     CDS             334..687
                     /gene="arsR"
                     /locus_tag="L2X49_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBN4799210.1"
                     /translation="MPEIASLQLFKILSDETRLGIVLLLREMGELCVCDLCTALEQSQ
                     PKTSRHLAMLRESGLLLDRKQGKWVHYRLSPHIPSWAALVIEQAWLSQQDDVQAIARK
                     LASANCSGSGKAVCI"
     gene            735..1097
                     /gene="arsD"
                     /locus_tag="L2X49_004213"
     CDS             735..1097
                     /gene="arsD"
                     /locus_tag="L2X49_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HBN4799211.1"
                     /translation="MKMLTVFDPAMCCSTGVCGSDVDQVLVNFSADVQWLKGRGVQIE
                     RYNLAQQPMSFVENEKAKAFLEASGAEGLPLLLLDGETVMAGRYPKRAELARWFGIPL
                     EKVGLASTSCCGGKTSCC"
     gene            1115..1351
                     /locus_tag="L2X49_004214"
     CDS             1115..1351
                     /locus_tag="L2X49_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN4799212.1"
                     /translation="MKFLENIPSYLFFTGKGGVGKTSISCATAIRLAELGKRVLLVST
                     DPASNVGQVAEAMAMVRALNRMTKAGMPESVRIA"
     gene            1438..2109
                     /locus_tag="L2X49_004215"
     CDS             1438..2109
                     /locus_tag="L2X49_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799213.1"
                     /translation="MKMLVEQAEANISRALIDADKPEAVESGEFPDEQHHEDGRITTL
                     PSTYYSCGSCFGYDEDEVRSEYKHLIVQLTRRSVFLTIFGLFEHRMVDCLALMDDLSS
                     KTTDKTRKTIEDCHKRLKRNFGGKSIKDVDHLAVIRNIMAHSDGVAEDYKTLSCKKTK
                     KTEYEKPFLRAIPRAMAENAGVSINMFNDILMDDRFLMYAVGEFERYVNELNTAVRTY
                     QSRLT"
     gene            complement(2501..4000)
                     /locus_tag="L2X49_004216"
     CDS             complement(2501..4000)
                     /locus_tag="L2X49_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBN4799214.1"
                     /translation="MANIITCKTKDGKTIQYVDEVIGSGSMKDVYFSPDKSYVVAFYH
                     KPQNEQARERINMITGRYRQNIFEQTGGDYWKGLFCWPTDVVEHADKVGIVVPAYQQH
                     FFFRYGSKNNDFLGIKGREKEGKWFASANNQNKFLDPRERGNTLNYLKVCILLTRAVR
                     RMHAAGLCHSDLSYKNVLIDPELGHACIIDVDGLVVPGKFPPDVVGTPDFIAPEVVKT
                     SHLPKDDPRRVLPSIATDRHALSVLIYMYLLFRHPLRGGKIHDIDDEVRDEALSMGER
                     ALFIEHPTDRSNAVKVNQVSSFSLPWADPQKIPYTIMGPYLKPLFDRAFIDGLHDPSK
                     RPTADEWESALVKTVDLIQPCQNNDCDQKWYVFNGKTKPVCPYCGTPYKGKLPILNLY
                     SSRKAGSFRPDDHRLMVWSGQSLYAWHVNRLIAPNERTTDEQKKRVGYFVFHNDQWWL
                     VNEGLSGLISLPDRKTIGIGEKLLLEDNTQFILSSEDGGRLVVVQLLNN"
     gene            complement(4028..5761)
                     /locus_tag="L2X49_004217"
     CDS             complement(4028..5761)
                     /locus_tag="L2X49_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HBN4799215.1"
                     /translation="MSEFITPEHKIIALILEASGEPTDADKVFCLGNIPDVALAVRQL
                     REMVIQHSASEAIPAENTQTVSERLTQPFSDNILPPQNAGESEADRPEIEPVHAATAL
                     QGNDKQEAHSEMVDDKVSEGVQGHTEEVPDETGVLQTEASVTVQPEDMDKTSPVVSDN
                     LSPDTAVSALRPGEVPQRVENKTSPAPGKVPEVFIILPNARAGEPYDVRLEIATDTGV
                     EAEITEVNFSQDIGLIFDINERRLQGTPGHSGDFELTVKWSCSSHRDNEKKLLFVVNP
                     DPKSLWKVIDPPSDAPFYKKSIDHQSVCRSGIHIAGASRRGRSHEHVGSFRDDDYYLN
                     FSDVSGWSVLLVADGAGSAKYSREGSRIVSETVGNYLFNQFKTEKGLEIKNRVLRWNS
                     EDQRAVWEFMNHHFRQAAVLAVNNISSAAILAEDKVKSFSTTLLATVTFRDGADLFAA
                     SFWLGDGAIAAYGPAGKVRVLGTPDSGEYAGQTRFLDADAVSDAGFSKRISIGKWNDI
                     SHLVLMTDGVSDPWFETDNGLQNPQKWDRLMAELSPLLTDPEHASAQLVEWLNFFSPG
                     NHDDRTIIVLW"
     gene            complement(5761..6801)
                     /locus_tag="L2X49_004218"
     CDS             complement(5761..6801)
                     /locus_tag="L2X49_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBN4799216.1"
                     /translation="MRRLPVFFVLDCSESMIGENLKRMNDGLQKIVGDLRKDPHALET
                     VWVSVIAFAGVARTIVPLHDIVSFYPPRLPVGGGTNLATALRELTTQIDSQVRKTTQE
                     EKGDWKPVVYLLTDGRPTDDISAEVKRWNEFYAKKLNMIAVGIGSSVDLSVLRQLTEN
                     VLSFTENQEGDFSRFIQWITASVTAHSRSVGEDSQPPLKQTDYIVKLAKDDTARAYDE
                     NCVVLTGRCSKTRRPYLIKYERPPARVSGLAFDLNLNSFNIAGCYPVDEDYFSWTDMA
                     VAGAQINTSELHGVPGCPHCGNSSAFAMCTCGKLLCIDGPDDVNCPWCGKGLSFQSEG
                     GNSDFNVSRGRG"
     gene            complement(6894..7532)
                     /locus_tag="L2X49_004219"
     CDS             complement(6894..7532)
                     /locus_tag="L2X49_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBN4799217.1"
                     /translation="MRRLPVYLLIDTSGSMRGESIHSVNVGVQAMLSALRQDPYALES
                     VHISIITYDNEAREYVPLTALADFQFSDIEVPSAGGTFTGAALECLIQCVDRDIRRSD
                     GEQKGDWRPLVFLMTDGTPSDAWAYGEAVKEVQRRSFGSIIACAVGPKAGHEHLKLLT
                     DKVVALETLDSTAFSGFFKWVSASVASGSSSAGVTTDSDTLPPPPPEIQLVL"
     gene            complement(7533..8174)
                     /locus_tag="L2X49_004220"
     CDS             complement(7533..8174)
                     /locus_tag="L2X49_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBN4799218.1"
                     /translation="MTVSLRKGQGVSLRKNEFDLSSVTIGLGWDINEEKKGFLGGIFG
                     KKEEEYDLDVIAFLCNAAGKVTDLGNVENGKPTLVNGDIVFFNSLRHKSGQIWLTGDN
                     RTGAGDGDDEQIIVKLNSIDPKYEKIVFIVQIYNGEELQQHFGKVQNAFIRAVDGKNV
                     EMARFDLSGGVAFNGQRSMVFAELVRESSGWKLNAIGEPSTSDSFVSHLRNYI"
     gene            complement(8199..8837)
                     /locus_tag="L2X49_004221"
     CDS             complement(8199..8837)
                     /locus_tag="L2X49_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBN4799219.1"
                     /translation="MRRLPVYLLLDTSGSMHGEPIEAVKNGVQTLLTTLKQDPYALET
                     AYVSVITFDSTARQTVPLTDLLNFNLPSFSASGTTALGEALSLTANRIDAEVQKTTAE
                     TKGDWRPLIFLMTDGGPTDDWRKGLNEFKAAKKGVVVACAAGHDADTGVLKEITEIVL
                     QLDTADSSSIKAFFKWVSASVSVGSQKVDAGKKEVIGLDDLPPPPPEVNVVL"
BASE COUNT         2280 a         2162 c         2071 g         2379 t
ORIGIN      
        1 ttcttagttg tttgtccttt tcgttaatat ggaaagggtc gtatgtgttg gtattatagg
       61 ggtctgaggg ccaatggaac gaaaacgtac gctaaggaaa taaccaattg ttataattga
      121 tattattcat tcttctctat gaactgaaag cccgcactaa gcaatgtttt agcctcaaat
      181 gactttttta cctacccaac taacgtaacg atcgcacatc aggtcgtttt tcaaccgcat
      241 agtgacacct gcactcacat gcgagaaaac acatatgatt ttgacttctt gttttggtgg
      301 agagacaata cccattcata gcaggagtac ataatgccag aaattgcatc cctacaactt
      361 ttcaaaatac tatctgatga aacccgcctg ggtatcgtgc tactgctcag ggagatgggg
      421 gagctgtgcg tatgcgatct ctgcacagcg ctggaacaat cacaacccaa gacctcccgc
      481 catctggcga tgcttcggga aagtggcctg ttgctggatc gcaagcaggg gaaatgggtt
      541 cattatcgct tatccccgca tattccttcc tgggctgccc tggtgattga gcaggcctgg
      601 ttaagtcaac aggacgacgt acaggccatc gcccgtaagc tggcatccgc caactgctct
      661 ggcagcggta aggctgtttg tatctaaaaa ttttgcctgt acatatatgt ttttacaaat
      721 atgatgtgtt aaagatgaaa atgttaacgg tgtttgatcc ggcgatgtgc tgcagtaccg
      781 gcgtttgtgg ttcagatgtc gatcaggttt tggtcaattt ttctgctgat gtgcaatggc
      841 tgaaagggcg tggggttcag atcgaacgtt ataacctggc gcagcagcct atgagcttcg
      901 ttgagaacga aaaagcgaaa gcattccttg aggcttctgg agctgaaggg cttccgctgc
      961 tgttgcttga tggtgaaacg gtgatggccg ggcgataccc aaaacgcgct gaactggctc
     1021 gctggtttgg tataccgctg gaaaaggtgg gtttagcttc caccagttgc tgtggtggta
     1081 aaacttcctg ttgttgagta tgtcaggagg acatatgaaa ttcttagaga atatcccgtc
     1141 ttacctgttt ttcaccggta agggaggtgt aggcaaaact tcgatttcct gcgcaacggc
     1201 tatacgtctg gctgaactgg gaaagcgtgt tctgcttgtc agtaccgacc cggcttccaa
     1261 tgtcggtcag gtagcggaag ctatggccat ggtgcgtgcg ttgaacagga tgacaaaggc
     1321 cgggatgcca gaaagcgtgc gtattgcctg aaaatccagc cagctacagg gtcgttcgca
     1381 cgaaatctta tttattcaac aaagccgtta caggaagcaa tctcttcaat acctgtcatg
     1441 aagatgcttg ttgagcaggc agaagcgaat atatcccgtg cactaattga tgctgataag
     1501 ccagaagccg ttgagtctgg ggaatttccg gatgaacaac atcatgaaga tggtcgaatt
     1561 acaacgcttc caagtactta ctattcatgc ggttcgtgct ttggctatga tgaagatgaa
     1621 gttaggtctg aatacaaaca tctgatcgtg cagctgaccc gccgctccgt atttcttacc
     1681 atttttggtc tgttcgaaca ccgtatggtt gattgcctcg cactaatgga cgacctttca
     1741 agcaagacca cagacaaaac gcgcaagacc attgaagatt gccacaagcg tctcaagcgc
     1801 aacttcggcg gtaaaagcat caaggatgtc gatcacctgg ctgttatccg aaacatcatg
     1861 gctcatagcg atggtgttgc agaagattat aagactcttt cctgtaaaaa aacaaaaaaa
     1921 actgaatatg aaaaaccttt cctgcgtgct attcctcgtg cgatggctga gaacgccggt
     1981 gtgtcaatta acatgtttaa tgacatactt atggatgatc ggtttctgat gtatgccgtc
     2041 ggtgaatttg agcgctatgt taatgaactg aacactgcag ttcgcactta tcaaagcagg
     2101 ttgacctgaa ggaaccgtct gcttatacgc tcagttatga ggtaaagtaa gcaattcaca
     2161 gtctggatat tcatttaacg gagttattat atataagttg cagactactc gctaaatttg
     2221 gtgataaagg tgcaattaac tcaggtaaaa cgatccctcg tgtaggctga gttattggtg
     2281 aggcttttga tggagtttca accaatcttg ttggtaatat agcgaggaag cccttcattg
     2341 aaggaaatat aatcaatgat gtctaaagat ggtctgagta tgtcgaatac tgaattgaaa
     2401 aattagtata cccgtaatgt tgcgtttcga ttgtgataat tatggcccac ctaatggtgg
     2461 gctcatattg acgttatgat atcaatggcc agtaatatat ctaattattc aaaagctgaa
     2521 ccaccaccaa tcgcccacca tcctctgaag acaggatgaa ctgggtgttg tcctccagta
     2581 gcaacttttc accaatgccg atagttttcc gatcgggaag tgatatgagc ccgctaagcc
     2641 cctcgttaac cagccaccac tggtcattat ggaagacgaa ataaccaacc cttttcttct
     2701 gctcatcggt ggttcgttcg ttaggagcaa tcagtcggtt gacgtgccat gcatataatg
     2761 actgaccgct ccacaccata agccggtgat catctggcct gaaacttcca gcttttctcg
     2821 acgaatacag attcagaatg gggagtttgc ctttgtacgg tgtaccgcaa tagggacaaa
     2881 cgggtttggt cttgccatta aaaacgtacc atttctggtc acaatcattg ttctggcatg
     2941 gctggatgag atcgacggtt tttacaaggg cgctctccca ttcatcagct gtcgggcgct
     3001 tcgacggatc atgcaacccg tcaataaaag cccggtcgaa gagaggtttc aggtagggcc
     3061 ccataatggt gtacgggatt ttctgaggat ctgcccacgg gagcgaaaac gatgagacct
     3121 gattaacttt aactgcattg cttctgtcag tcgggtgttc gataaaaagt gcccgctctc
     3181 ccatggaaag cgcctcatcc ctgacctcat catcgatatc gtgaattttt cctccccgta
     3241 acgggtgccg gaaaagaaga tacatgtaaa tgagtaccga aagtgcatgc cggtcggtag
     3301 cgatactcgg caatacgcgg cggggatcgt ctttggggag atggcttgtc ttcaccactt
     3361 ccggggcaat aaaatcaggt gtaccaacca cgtctggagg aaattttccc ggaaccacca
     3421 gcccatcaac atcaataatg catgcgtggc ctagttccgg atctatcagc acatttttat
     3481 aactcaggtc actatgacac aggcccgcag catgcattcg tctgactgca cgcgttagaa
     3541 ggatgcagac cttcagatag ttaagcgtat tgccacgttc acgcggatcc agaaatttat
     3601 tctgattgtt cgcgctggca aaccatttcc cttccttttc acgtcctttg atcccgagga
     3661 aatcattgtt tttggaacca tatcggaaga agaaatgctg ctggtaagca ggtacaacaa
     3721 tacccacctt atcagcgtgc tcaaccacat cagtgggcca gcagaagaga cctttccagt
     3781 aatctccacc cgtctgctca aaaatattct gccggtacct cccggtgatc atgttgatac
     3841 gttcccgggc ctgttcattc tgaggtttat ggtagaaggc tacgacatat gatttatccg
     3901 gagaaaaata aacgtctttc attgagccag agccgatgac ctcatcgaca tactgaatgg
     3961 tttttccatc cttagttttg catgtaatta tatttgccat gaatagccac ctgctatcgc
     4021 attaatttca ccagagcaca atgattgtgc gatcatcgtg atttcccgga gagaaaaagt
     4081 taagccactc caccagttgt gccgaggcat gttcagggtc tgtgagtagg ggagagagct
     4141 cggccatcag cctgtcccat ttttgtggat tctgaagccc gttatccgtc tcaaaccagg
     4201 gatcggaaac accatcagtc atcagaacca gatgcgatat gtcgttccac ttaccgatac
     4261 tgatacgttt actgaatccg gcatcactga cggcatcggc atccagaaac cgcgtctgac
     4321 ctgcatactc tccgctatcc ggtgtcccaa gcactctgac tttgccagca ggtccatatg
     4381 cagctatcgc accgtcacct aaccagaaag acgcggcaaa cagatcggca ccgtcgcgaa
     4441 aagtgacggt cgcgagtaaa gtcgttgaga atgactttac tttatcttca gccaatattg
     4501 cggcactgct gatattgttt acggccagta ccgctgcctg tcggaagtgg tggttcataa
     4561 actcccatac tgctcgctga tcctcactgt tccagcgaag gaccctgttt ttgatctcaa
     4621 gacctttttc agtcttgaac tggttgaaga gatagttacc aacagtttca gaaacgatac
     4681 gggagccttc ccgggaatat ttagcgctcc ccgcgccatc agctaccagt aatacactcc
     4741 atccggatac atcactgaag ttgaggtaat aatcgtcgtc acgaaaagag cctacatgtt
     4801 catgggagcg tccacgacgg cttgctccgg caatatgaat acctgaccga cagacactct
     4861 ggtggtcgat actcttcttg tagaatggtg catcagaagg cgggtcaatc actttccaga
     4921 gacttttcgg atcagggttc acgacaaaaa gtaacttttt ttcattatca cgatgtgagc
     4981 tacatgacca tttcacagtc agttcaaagt ctccgctgtg ccctggcgtc ccctggaggc
     5041 gtcgctcatt gatatcaaat atcagcccta tgtcctgaga gaagttaacc tccgtaatct
     5101 ctgcctcaac acctgtatca gtcgctattt ccagtcgaac atcgtagggt tcacctgccc
     5161 gtgcatttgg cagaatgata aacacctccg gcacttttcc gggggccggg gatgttttat
     5221 tctccactct ctgaggtact tccccgggcc ttaaagcact gactgcggtg tcaggtgaaa
     5281 ggttgtcaga aacaaccgga ctggttttat ccatgtcttc tggttgcacc gtgacggaag
     5341 cctcagtctg taagaccccg gtttcatcag gaacttcttc agtgtgtccc tgaacgcctt
     5401 ctgacacttt atcatccacc atttcactat gcgcttcctg cttatcattt ccctgaagtg
     5461 cagtagcggc atggacgggt tcaatctcag gtcgatcagc ttcgctttca ccagcgtttt
     5521 gaggtgggag gatattgtca gaaaagggct gggtaagacg ttctgaaact gtttgagtgt
     5581 tctccgccgg tatggcttcg cttgcagagt gttgaatgac catttctctc agctgccgca
     5641 cagcaagggc aacatcaggt atgttaccaa gacagaatac tttatctgca tcagtcggtt
     5701 cccctgatgc ctccagaatg agagcaataa ttttatgttc aggtgtaatg aactcgctca
     5761 ttagccgcgt cctctgctaa cgttaaaatc tgaatttcca ccctcgctct gaaaggacaa
     5821 acctttccca caccatgggc agttcacatc atcaggccca tcaatacaga gaagttttcc
     5881 gcaggtacac atcgcaaaag cactactgtt tccgcagtgt gggcaccctg gtacgccatg
     5941 gagttcactg gtgtttatct gcgcccccgc tacggccata tccgtccatg agaagtaatc
     6001 ttcatccaca ggataacaac cagcaatgtt aaagctgtta aggtttagat cgaaagcgag
     6061 tcccgatacc ctggctggcg gacgctcata cttaatcaga tagggtcgac gggttttact
     6121 gcaacgcccg gttaatacca cacagttttc atcgtatgca cgggctgtgt catctttggc
     6181 aagtttgacg atatagtcag tttgtttaag gggcggctga gaatcctcgc caacactacg
     6241 actatgagcc gtgactgaag ctgtgatcca ctggataaac cgcgaaaaat cgccttcctg
     6301 attttccgta aaggataaga cgttttcagt cagttgcctg agtacactga gatcgaccga
     6361 tgatccgatc cccacagcaa tcatgttcag tttttttgcg taaaattcgt tccagcgctt
     6421 aacttcagct gaaatgtcat ctgtcggtcg accgtcagta agtaagtaga caactggctt
     6481 ccagtcacct ttttcttcct gcgtcgtttt tcggacctga gaatctatct gtgttgtcag
     6541 ttctcgtagg gcagtcgcga ggttagttcc tccaccgaca ggaaggcggg ggggataaaa
     6601 agacacaatg tcatgtaacg ggacaatcgt tctggcaaca ccagcaaagg caataactga
     6661 cacccagaca gtctccagag catgagggtc cttacgcaga tcgcccacaa tcttctgcaa
     6721 gccatcattc atccttttca ggttttcacc aatcatggac tcagaacagt ccagaacaaa
     6781 aaacacggga agtcgtctca caggaaactc cctcatagca aaatgtgacg tccatggcag
     6841 agatggactt tatcggtata cggcagggtt attccctgcc ataccggcgt aagttacaga
     6901 acaagctgga tttcgggcgg tggcggtgga agcgtgtccg aatcagtagt gactccggca
     6961 ctggagcttc ctgaagccac gctggcactg acccatttga aaaagcctga aaatgcagtc
     7021 gaatccagtg tttcaagagc cacaactttg tcagtcagta gtttcagatg ttcgtgtccc
     7081 gctttaggac caacagcaca ggctataatg gaaccaaacg aacgccgctg aacctctttc
     7141 acagcttccc cataagccca ggcatctgac ggagtaccat ctgtcatcag aaacaccagc
     7201 ggtctccagt cgcctttctg ttcaccatca gagcgtcgaa tatcccggtc cacacactgg
     7261 atcaaacatt caagcgccgc gccggtaaac gtaccgcccg cgcttggtac ttctatatca
     7321 gaaaactgaa agtccgccag ggcagtgaga ggcacatact cccgggcttc attatcgtat
     7381 gtaattattg agatatggac gctttcgagc gcataagggt cctgtctcag ggcactcagc
     7441 atagcctgca cacctacatt cactgaatgt atcgattctc ctctcattga gccggaagta
     7501 tcgatgagca gatatacggg tagtcttcgc atttagatat agttcctcag atggcttacg
     7561 aacgaatcgg aggtggaagg ctcgcctatc gcattcagtt tccagccact ggattcccgg
     7621 acaagctcag caaagaccat cgatcgctga ccattaaagg ctacaccgcc ggagaggtca
     7681 aagcgtgcca tttccacatt ttttccgtct acagcccgga taaatgcatt ttgcactttt
     7741 ccaaagtgct gctgtaattc ttcgccgttg tagatctgaa caataaagac aattttttcg
     7801 tacttcggat cgatgctgtt aagttttacg ataatttgct catcgtctcc gtccccggca
     7861 cctgtccggt tatcgccggt caaccagatc tgtcctgatt tatggcgaag actgttgaag
     7921 aagacgatat cgccgttaac cagtgtcggc tttccatttt caacattgcc aaggtcagtc
     7981 actttaccgg ctgcgttgca gagaaaggca ataacatcga gatcatactc ctcctctttt
     8041 ttgccaaaaa ttccgccaag gaaacctttt ttctcttcat tgatgtccca gccaagacct
     8101 atcgtgactg atgaaagatc gaactcattt ttacgcaggc tgacgccttg ccctttccgt
     8161 aagctgacag tcataggatc ctctctttag ttggttactt ataaaactac gtttacttct
     8221 ggcggcggtg gaggcaaatc atcaagaccg ataacttctt ttttaccagc atcgactttc
     8281 tggctaccta cggaaacact ggcagacacc catttgaaaa aagctttgat ggaagaactg
     8341 tctgccgtgt ccagttggag aacaatctct gtaatctcct taagtacccc tgtgtcggca
     8401 tcgtgaccgg cagcacaggc aacaacgaca ccttttttgg ctgctttgaa ttcattcagt
     8461 cctttacgcc agtcatcagt agggccacca tccgtcatca ggaaaatgag tggacgccag
     8521 tcgccttttg tctccgctgt cgttttctga acttctgcat caattcggtt tgcagtcagg
     8581 gataaggctt cgcccaaagc ggtggttccg cttgcagaaa aggagggcaa attgaaattg
     8641 aggagatccg taagaggaac ggtctgacgg gcagttgagt caaaggtaat gacagagaca
     8701 tatgcggttt ctaaagcata tggatcttgt tttaacgtgg tcagaagggt ctgaacacca
     8761 ttttttacag cctctatagg ttctccgtgc atcgaacctg aagtatccag aagcagatag
     8821 acgggtagtc ttctcatctt tgacattcct taacgatttg gcgtacaagc acattgcttg
     8881 ttcatcatta ta
//
LOCUS       DAFNBJ010000184         8869 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000184 DAFNBJ010000000
VERSION     DAFNBJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.184"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(8..1096)
                     /locus_tag="L2X49_004222"
     CDS             complement(8..1096)
                     /locus_tag="L2X49_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN4799220.1"
                     /translation="MITTLHIQNYRSIREMSLELEQLNIVFGPNGTGKSNIYKAIHLM
                     HSAAQGQFSQALANEGGILKVFWAGKTRSDQLRRMNLAVETETYEYELQVGFVEKLPY
                     PSQFQLDPVIKEESIWLSGQYRRPSSQLMRRKNQAVFLNNVHHEKVTHSGTLYENESV
                     FGQLGEPHLYPEVSQMRESLRNWRFYQEFSVSIGSAMRAPQVGFRSPVLASDGANLAA
                     AFQTIVEIGDELLLMRILDQAFPGCVFYSDNTGGRFRMMMQREGLSRPLEPAEFSDGT
                     LRFLCLAVALLSPRPPAFIALNEPENSLHPQMLPALASLIAEASRYSQIWLTSHSPEL
                     ANLIEKQRSFSLYQLSMVEGETKVERLG"
     gene            complement(1242..2201)
                     /locus_tag="L2X49_004223"
     CDS             complement(1242..2201)
                     /locus_tag="L2X49_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HBN4799221.1"
                     /translation="MTTKPVLGISGCLTGSAVRFDGGHKRMGFVMDELAQWVSFRPVC
                     PEMAIGLPTPRPAIRLTLTDSGETQLRFSKPPHDDITQKMADFTADYLPKIGDLSGFI
                     VCAKSPSCGMERVRLYDENGNRGRKEGVGLFTAALLETYPWLPVEEDGRLHDPVLREN
                     FIERVFALHELNTLRAKGLTRRALLDFHSRYKLQLLAHHQAGYREIGPFVASLHEWED
                     LDAFFVAYREKLMTILKKPASRKNHTNVLMHIQGYFRNQLNSRQRVELRDVILHYRDG
                     LLPILAPLTLLKHYMAEYPDRYLMTQNYFDPYPDDLGLRLAVT"
     gene            complement(2304..2714)
                     /gene="uraH"
                     /locus_tag="L2X49_004224"
     CDS             complement(2304..2714)
                     /gene="uraH"
                     /locus_tag="L2X49_004224"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBN4799222.1"
                     /translation="MKKTASLLMLTTLAFAPAAFSAPAGTLSVHILDQQTGMPPSDVT
                     VTLEKQQQDKWTPIASGKTDHDGRIKSLYPEDQDMQPGVYKVAFKTADYFHGKKLDSF
                     FPEIPVLFTVTRTNEKLHIPLLLSQYGYSTYKGS"
     gene            complement(2814..3539)
                     /locus_tag="L2X49_004225"
     CDS             complement(2814..3539)
                     /locus_tag="L2X49_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN4799223.1"
                     /translation="MAYSIGEFARLSGITATTLRAWQRRYGLLKPQRTEGGHRQYSDE
                     DVQQALKILDWVKKGVPIGQVKPLLERPTSRLTNNWLTLQQSMLQRLQDGKIESLRQM
                     IYDAGREYPRPELVANVLRPLRSQVSANVAAAMTLREILDGIIIAYTSFCLEGDKKAP
                     GDNILLSGWNLNDPCEVWLEALTRTGQGHRIDILPTPPATLAPEIFPDRHWLLVTRGK
                     LTAARKKQIEQWQQQVSLEVIIL"
     gene            3657..3986
                     /locus_tag="L2X49_004226"
                     /pseudo
     CDS             3657..3986
                     /locus_tag="L2X49_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
     gene            complement(4238..6217)
                     /locus_tag="L2X49_004227"
                     /pseudo
     CDS             complement(4238..6217)
                     /locus_tag="L2X49_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6214..7884)
                     /locus_tag="L2X49_004228"
     CDS             complement(6214..7884)
                     /locus_tag="L2X49_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBN4799224.1"
                     /translation="MTKTSHSKIYSTESGITGGGFFKTHPLHFTKEQLEWIENIQLPK
                     RLFLISATEFDDRYLYLHDDWRYRFSGNNLAILFSTGKYFCDLSPVSLNLLKYLTIAY
                     INENSASVADKFAQFVATTFKQIEVITRESLIEKMQMLVAKTERNHSDSSQFYYTLYA
                     LRKLDRNGFFESKDDSDELEDLLLEVPRPRNNNWARYQNLDLVIPEEVCLMIENGIQR
                     WASKLCPKIDVEENKNIHLETVRKTIKIDTLRDCIIIGIVYYIGARPVQIGKLSGGDF
                     IVDTENKYGMRYSVLVPYAKKSKLTIDRVRVAIPEELGKLILLYKYLVGIGDTDPMFS
                     IIVSSMKMVEASLKKMLFRFSPQEIQEAVKNDDYQLPVYTASLFRHNVGHSMAMSGAS
                     APEIAYILGQSSYVVADRYIAATPELADIREAALGRNPVFKNMMALMLTGNLVHSSEW
                     EGRNVAGSIGGQLHYHLGGCNYEEDICPFAQGRGCYGCLYFKPFIDGNHKKVFLSLND
                     EIQNVRDVADDAGILNHPLLKELVRRKEHVNQVMARIEMAKAGGLFNR"
BASE COUNT         2207 a         2110 c         2109 g         2443 t
ORIGIN      
        1 tgggagacta ccccagcctc tccaccttcg tttccccttc caccatcgac agctgataca
       61 gcgaaaacga tctctgtttc tcaattaaat tcgccagttc cggcgagtgg ctggtcagcc
      121 aaatttgcga gtagcggctg gcctcggcga ttaagctcgc cagcgcgggc agcatctgcg
      181 gatgcaggct gttttccggt tcgttgaggg cgataaaggc cggtggacgc gggcttaata
      241 gcgccaccgc caggcacaga aaacgcagag tgccgtcgga gaattccgcc ggctccagcg
      301 gtctgcttag cccttcacgc tgcatcatca tccggaagcg tccgccggtg ttgtcgctgt
      361 aaaacacgca gccggggaag gcctggtcga ggatgcgcat cagcagcagc tcgtcgccaa
      421 tttccacgat ggtctgaaac gccgccgcca ggtttgcgcc gtcgctggcc agcactgggg
      481 agcggaaacc gacctgaggc gcgcgcatgg ccgagccgat tgatacagaa aactcctgat
      541 aaaagcgcca gttacgcagc gactcgcgca tctgcgacac ttccgggtaa agatgcggct
      601 cgccaagctg gccgaacacc gattcgttct catacagcgt gccgctgtgg gtgactttct
      661 cgtgatgcac gttgttcaga aatacggcct gatttttacg cctcatcagc tgtgacgacg
      721 ggcggcggta ttgaccactc agccagatgg actcttcttt gatcaccgga tcgagttgaa
      781 actgtgaggg gtaaggcagt ttttcgacaa agcctacctg cagctcgtat tcgtaggttt
      841 cggtttcaac ggccaaattc atccgccgca gctggtcgct gcgggttttg cctgcccaga
      901 acacttttaa aatcccaccc tcgttcgcca gtgcctgcga aaactgcccc tgtgcggcgc
      961 tgtgcatcag gtggatcgcc ttgtagatat tggattttcc ggtgccgttt gggccaaaga
     1021 cgatgttgag ctgttccagc tccagcgaca tttcgcgaat cgagcggtag ttttgaatgt
     1081 gcagggtggt gatcacaggc agtccctttt tgcgttacaa tcctgtacat aattacagtc
     1141 ttttgtgtgg aatgcaaaaa agagacgaca gtgagggtgt cgtctaaacg gggtactgct
     1201 ttgggcctct gtgggtcaaa cgggccttcg catttttatc gtcaggttac tgccagacgc
     1261 agacccaaat catcaggata gggatcaaag tagttttgcg tcatcagata ccggtcaggg
     1321 tattcggcca tgtagtgttt cagtagcgtc aacggcgcga ggatgggtag cagcccgtcg
     1381 cgatagtgca ggatcacatc gcgcagctcg acgcgctggc ggctgttcag ctggttacgg
     1441 aaatagccct ggatatgcat tagcacgttg gtatggtttt tccgcgaggc gggctttttc
     1501 aggatggtca tcagcttttc gcggtacgcc acgaagaacg cgtccagatc ttcccattca
     1561 tgcagcgacg caacgaacgg gccaatctcg cggtagcccg cctgatggtg cgccagaagc
     1621 tgcaatttat agcggctgtg gaaatccagc agcgcgcggc gcgtcaggcc tttagcgcgc
     1681 agcgtgttca gctcgtgcag ggcaaatacc cgctcgataa agttttcccg cagcaccggg
     1741 tcgtgcagcc gaccgtcttc ctccacgggc agccacgggt aggtttcaag cagggcggcg
     1801 gtgaacagcc cgacaccctc cttgcggccc cggttgccgt tttcatcata tagccgcacg
     1861 cgttccatgc cgcagcttgg cgatttcgcg cagacgataa atcccgacag atcgccgatt
     1921 tttggcagat agtccgccgt aaagtcggcc attttctgcg ttatatcatc atggggcggc
     1981 ttgctgaagc ggagctgcgt ttcgccgctg tccgtcagcg tcaggcgtat ggccgggcgt
     2041 ggcgtcggca ggccaatcgc catttccggg cagacgggcc tgaaactcac ccactgggcc
     2101 agttcatcca tgacaaagcc catacgcttg tgtccgccgt cgaagcgaac ggcagacccg
     2161 gtcaaacatc cgctaatacc gagcacaggt tttgttgtca tggtgtggtc ctcccgttct
     2221 tctctttaaa acttacacaa catgactaat ttgtccaagt attagcggaa ttatttttta
     2281 tttgccttta aaatcaacgt ttcttaactg cccttgtaag ttgaataacc gtattggctc
     2341 agcaggagag ggatgtgcag cttttcgttg gtccgggtta cggtgaacag caccgggatc
     2401 tccgggaaga aggagtccag cttcttcccg tggaagtaat ctgcggtttt aaaggccact
     2461 ttatagacgc cgggctgcat atcctgatcc tccggataaa gcgatttgat gcggccgtca
     2521 tggtcagttt tgccgctggc aattggggtc catttgtcct gctgctgttt ttccagcgtg
     2581 acggtaacgt ccgatggcgg catcccggtt tgctggtcaa gaatgtgtac gctcagcgtg
     2641 ccggcagggg cgctaaaggc ggcaggggca aaggccaggg tggtcagcat cagcagagag
     2701 gcagtttttt tcattgtgtt ttcctgtggg gttgtcggtc acaggaaaac gttagcattt
     2761 gtggcgcggg aaaatattca acttttcgtg aagattcccg caggagaaaa tgattagagg
     2821 ataatcactt caagcgatac ctgctgctgc cactgctcga tctgcttttt gcgcgcggcg
     2881 gtgagtttac cgcgcgtgac cagaagccag tggcggtccg ggaaaatctc cggggccagc
     2941 gtggcgggcg gcgtgggtaa aatatcgata cggtggcctt gcccggtgcg ggttaaggct
     3001 tcgagccaga cttcgcacgg gtcattcagg ttccagccgc tgagcaggat gttgtcgccc
     3061 ggcgcttttt tatcgccttc caagcaaaac gaggtgtagg cgatgatgat gccgtcgagg
     3121 atctcccgca gggtcatggc ggcggcgacg tttgccgaca cctgactgcg cagcggacgc
     3181 agcacgttcg cgaccagttc tggtcttggg tactcgcgtc ctgcgtcgta aatcatctgg
     3241 cgcagggact cgattttgcc gtcctgcagg cgctgtaaca tgctctgctg cagcgtgagc
     3301 cagttgttgg tcaggcgcga cgtagggcgc tccagcagcg gtttgacctg gccaatcggg
     3361 acgccttttt ttacccagtc gaggattttt agcgcctgct ggacgtcctc atcgctgtac
     3421 tggcggtggc cgccctccgt gcgctgaggc ttgagtaagc cgtagcgccg ctgccaggcc
     3481 cgcagcgtgg tggcggtgat cccgctgagt ctggcgaatt cgccgatgga gtaagccatg
     3541 tccgtgtcca gagttgagga atactctcaa tatactacga attttcgcgg atgggatgaa
     3601 gcgccgccgc ggtgcactac acttaatttc caaccgtgca aatgaaagga attctcatga
     3661 agctatggcc tgttgtgacc ggtgttgcca ttgccctgac gctggtagcc tgtaaatccc
     3721 ctaccccacc gaagggcgtg cagcccattt ccggttttga cgccagccgc tatcttggca
     3781 aatggtacga agtcgctcgc ctggaaaacc gctttgaacg cggcctggag caggttaccg
     3841 ccacctacgg caagcgcagc gacggcggca tcagcgtgct taaccgcggc tacgaccctg
     3901 tgaagaacaa gtggaatgag agcgagggta aagcctactt taccggcgaa ccgaccacgg
     3961 ccgcgctgaa ggtgtccttc ttggggtcag tttggatata gagaattatt gtacggtaag
     4021 cctgtttttt gaacaggctt acctgtagca atagggtgta ctcccgcgct atacggcatt
     4081 ttcagaggtt tgaccttaat cggataacct aaatgcgata ctgcaagagc gacaacttaa
     4141 aagcgatagc cactgacgca aaatcaaccc cttacgaacc aagattggtt aaataatgcg
     4201 cttaacgtac aaaaatttcc gatctccaaa ctgaccccat atagtccaca acctcagcag
     4261 ttgtttcacc aaaagtctgc atgttctgct tcagattatg ctcggtattg agctcaggat
     4321 ttatcattag tgaggttgtt aatttaacgt ttccaacacc tgtgtttttt accgtcttgg
     4381 aagaccgtga tagagctgcc attctgcggc taacctcagt tctgggggag ggtaggttga
     4441 ccggaatgca gggcttctgc tgcgtagtaa agagtgatag ctggaaactc ttcgtgagag
     4501 cttgattgtt ctcatcataa gccttttcca gcatattgat tgctagagaa gcggtctgat
     4561 aggactgtga ttcggcctga tgctgatagt gtttattctg ggtgatatca acacgcccca
     4621 ttaggatggc ctgaatatga tccgtcactc cagcgattgc caagaatgta tttatgttat
     4681 gtctcggtac atgctttttg ataatgacac gggacccatc atcttcaaaa agattaaagt
     4741 gatcaaagat agattttcgg ttttgtgttc cgcctaacca agcttctatt gagttttcat
     4801 caagtggaaa aatatgttgc aatgaaacaa aatctttacc aattccaaat gcaccttccg
     4861 gcatgatgaa catcaggtct tcataattga agtgttccca gtcaccattg tttttggctg
     4921 aaattatgca ctggtgccca ggctcaaagt tttttgcttg agcgtaacgc tgatagacat
     4981 aatcattcaa ctgttcttta gtgaagtacc agctgttctt ctgtttatga acgggtatta
     5041 ccttaggttt atgtccgcta aagttcgcaa tggaattccg aatactcttt ccaagatcat
     5101 tcctggagcc accagaacca ctaaacatga tgtcttcaag ataattttgc tcaatccatt
     5161 catcaggaaa atcagcgatc ttacctggga gcatattagt gaaattagaa tctctgaagc
     5221 cctgaatcgt tttacggaac ccagaggtca attctcgaac agatttataa atcatctcaa
     5281 cgacagaata agtgcttggc gccagccaat ggatccgcca tccagccttt tttgcaccaa
     5341 gatactcaat acccaacctg taagtaggcc agtcttgatc tattgcgtgc tgacgtttat
     5401 ctttatccgt aatttctcgc ttaaccaaag aagtcatttt cagcaacatt atttcctgga
     5461 aacggaaacc gcaaataatt gtgagtaaca acatattgta aaagatacgc tgataactat
     5521 tctcagctaa agcgatcaac tcaataacac acataaatgc acggattgat atgagtcttt
     5581 catcatcagt ttgatcaggg gtggcttctt gcgctttctt cctttcttta gtgctgttag
     5641 aaccatttcg ataaggatac ttatttttga cttcaatatt aactaagaaa agatcatatt
     5701 gacgtagtaa agcaatggcc tttactgcaa tatcgcaata gtcactgaca ttatcaggac
     5761 tgtcagaatt ctcatgatgc cgctccatcg ctgcatgaat aatatcggaa gtcagataca
     5821 ttggatgcgt ttgacctgtt ttatttacca tttcccaata ccagcgcttc atgacctgta
     5881 atggtttgtc tagtgtagaa cctgatggta atccgccaat gatacacacc agatgcatgt
     5941 gataagctcg cataaaatcc tgaaattccg aagaaatatt catttgtgag agccccttaa
     6001 tacgactaga aacttcgtcc cacttaaagc cgcgatcttt tgtttcttta agccatgcaa
     6061 cacctttctc accaaaacgc caggaaccgg cattccactc cagttgtcca acagcattgt
     6121 aggcaacaag acgctcttca aattttgctt tatatttctc tatataaata agaagattat
     6181 ccgcctgttt attacgtatt gttatttgtg tagtcatcga ttaaatagtc ctccggcctt
     6241 tgccatttca atgcgagcca taacctgatt tacatgctct ttccgccgca ctaattcctt
     6301 gagaagtgga tgatttaaaa tgccagcatc atcggcaaca tcacgaacat tttggatttc
     6361 atcattcagc gaaagaaata cttttttgtg gtttccatct ataaatggtt tgaaatacag
     6421 gcaaccgtaa catccacgac cctgtgcaaa ggggcaaatg tcttcttcat aattacatcc
     6481 accgagatgg taatgaagtt gcccaccaat tgaccctgca acattgcgac cttcccattc
     6541 gctgctgtga actaaattcc cagtcaacat gagtgccatc atgttcttaa acacagggtt
     6601 gcgacccaaa gctgcctcac gaatatcagc cagctcaggg gtggctgcaa tgtatcgatc
     6661 tgcaacaaca taagaggatt gtcccaatat ataagctatc tcaggagcac tggcaccact
     6721 cattgccatt gagtgaccaa cgttgtgacg aaagaggctt gcggtatata caggtagttg
     6781 ataatcatcg ttttttactg cctcctgaat ctcttgtggt gaaaatctga aaagcatttt
     6841 ctttaatgac gcttccacca tcttcattga agatactatg atggaaaaca tcgggtcggt
     6901 atcaccaatt ccaactaggt acttatacag taagattaac tttccaagtt cttccggaat
     6961 tgcaactcga actcgatcga tagttagttt tgactttttg gcatatggca caagtactga
     7021 ataacgcatc ccgtacttat tctcggtatc aacgataaaa tcgcctccgg aaagtttacc
     7081 gatctgtaca ggtctggccc caatgtaata aactatgcca ataataatgc agtcccgtaa
     7141 tgtatcaatt ttaatggttt tccttacagt ttccagatga atgtttttat tttcttcaac
     7201 atcaattttt ggacatagct tagatgccca acgttgaatg ccattttcta tcattaggca
     7261 tacttcttct ggtataacga ggtctaggtt ttggtagcga gcccagttat tgttcctagg
     7321 gcgtggcact tccagcaata gatcttccag ctcatcactg tcatccttac tttcgaagaa
     7381 tccattacga tcaagttttc gtaatgcata gagggtgtaa tagaactgac tgctgtctga
     7441 gtgatttcgc tcagtcttcg caactagcat ttgcattttt tcaataaggc tttcgcgagt
     7501 gataacctca atttgtttga aagttgttgc gacaaactga gcaaatttat cagcaacact
     7561 tgccgagttc tcattaatgt acgcgatcgt taagtatttc aagagattga gtgaaactgg
     7621 tgataaatca caaaagtatt taccagtaga gaataatata gccaaattat tacctgagaa
     7681 tctgtaacgc caatcatcat gtagatataa atatcggtca tcaaattctg ttgcacttat
     7741 taaaaacaat cgtttaggga gctgaatatt ttcaatccat tcaagctgtt cttttgtaaa
     7801 gtgaagggga tgggttttaa aaaaaccacc cccggtgata ccgctctcag ttgaatagat
     7861 ctttgaatgt gaagtcttcg tcataaacat tatcttctag caagttggag gtttttcttt
     7921 gcgaaatgtt tgtttctggg gaagctaaat gcatatctga atgtttcgta cgcttatagt
     7981 tgctctggtt actcaccact tcaataaacc gcttggcata tcttaatgga accaaatgtt
     8041 tgtttatgct tcttatagta atagacgtaa aagaaacgca gcgaacacaa aatgttttct
     8101 tgagtcccta ggcttttctt gctcagttca ttcattccat aaattgtggc gaacatgacc
     8161 ggtagggtag tcacatcatc aactatgaga tgagttctct ggttgttcct cgataaatgt
     8221 aagatggtat atgccatgaa cttagcggat gccccgtaat ttttgtgtta tgaagtcatc
     8281 gtaagtccac aactaaactt tggcaactga ctgattcatc ggtaagttcc tgaaagagag
     8341 ggactcatga acttaagagc aaacccacca tatttaactt atgttgattg caacgctagt
     8401 tcactgacat cgcgccgatt aatgtattgt ttttataatt gatttccata aaaaaacata
     8461 atattttgtt acctcgcttc atgaactggt cctgcacaac ccgtatggat gtttttagct
     8521 gataggccta actacagttc attaatcatt tctcatcaca ttttcaaaac aatcatctca
     8581 ttgttttgat tagttatctg attgatctat tgaataaagt gtaggttgcg ctagcataga
     8641 gtttatcaag ggctcttgcc tctgagggcg gtagcattgt gccatcaaca ttgcacacgg
     8701 agcgctaagg caacggaatt ttaacaaatt aacaggtttt cctacaaaac tgtgaagcga
     8761 aaggttactc cctgacttga acaggtgaaa acatgctagg tctgaacagt tattcctgat
     8821 actgaatgaa taaatatgcc accttaagtg gagccaaaaa tggcaagaa
//
LOCUS       DAFNBJ010000185         8733 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000185 DAFNBJ010000000
VERSION     DAFNBJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.185"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..1114
                     /locus_tag="L2X49_004229"
     CDS             <1..1114
                     /locus_tag="L2X49_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN4799225.1"
                     /translation="IFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVARNMAISYEQDDL
                     LCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALITSEIFCRLARNPK
                     ITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLNDLIYTLIQTPLK
                     HMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENLSWAAQHGTTWGA
                     PYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSFIPGIFNINEPIM
                     FGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTTPGPLIAFLGTGG
                     NWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1142..1453
                     /locus_tag="L2X49_004230"
     CDS             1142..1453
                     /locus_tag="L2X49_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBN4799226.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1509..3182
                     /locus_tag="L2X49_004231"
     CDS             1509..3182
                     /locus_tag="L2X49_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HBN4799227.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            3207..4607
                     /locus_tag="L2X49_004232"
     CDS             3207..4607
                     /locus_tag="L2X49_004232"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBN4799228.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFY"
     gene            complement(4703..4921)
                     /gene="marB"
                     /locus_tag="L2X49_004233"
     CDS             complement(4703..4921)
                     /gene="marB"
                     /locus_tag="L2X49_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBN4799229.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4953..5336)
                     /gene="marA"
                     /locus_tag="L2X49_004234"
     CDS             complement(4953..5336)
                     /gene="marA"
                     /locus_tag="L2X49_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBN4799230.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(5356..5790)
                     /gene="marR"
                     /locus_tag="L2X49_004235"
     CDS             complement(5356..5790)
                     /gene="marR"
                     /locus_tag="L2X49_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBN4799231.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6002..6667
                     /gene="marC"
                     /locus_tag="L2X49_004236"
     CDS             6002..6667
                     /gene="marC"
                     /locus_tag="L2X49_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBN4799232.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6692..7882)
                     /gene="ydeA"
                     /locus_tag="L2X49_004237"
     CDS             complement(6692..7882)
                     /gene="ydeA"
                     /locus_tag="L2X49_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBN4799233.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGSGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGSAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8032..8733)
                     /gene="yneK"
                     /locus_tag="L2X49_004238"
                     /pseudo
     CDS             complement(8032..8733)
                     /gene="yneK"
                     /locus_tag="L2X49_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         2383 a         1878 c         2103 g         2369 t
ORIGIN      
        1 cattttcccc aaccttgaaa aagcacaagc tatttttact ccggtaatga atggttctgt
       61 aaatatcatg tcaattatga ttgctttcct ggtcgccagg aatatggcga ttagctatga
      121 gcaagatgat cttttatgcg gattaacggc aataggagca ttttttattg tttatacgcc
      181 atatcagatg atagatgggc aagcattcct gacgaccaaa tatctcggcg cgcaggggtt
      241 gtttgttgct gttatcgttg cattgatcac cagtgaaata ttttgtcgct tagctcgaaa
      301 cccaaaaatc accataacga tgccggcagc tgtaccgcct gcggtagcgc gttcatttaa
      361 agttttattg ccaatatttt ttgtcatggt gttcttttcc gcacttaatt attgcctgac
      421 actgatatcc ccggcaggac taaacgatct tatttacaca ttaatccaga cgccgctcaa
      481 acatatggga acgaatatct ttgcggtaat tatcctgggg gctgtgggta atttcctgtg
      541 ggtgctgggg atccacggac ctaataccac atcggcaatt cgagaaaccg ttttttctga
      601 ggctaatctg gagaatctct cctgggccgc tcaacatggc actacctggg gcgcgccata
      661 tccgattacc tggacttcta ttaatgatgc attcgccaac tgcggcggtt caggtatgac
      721 gttggggtta ttgttggcta tttttatcgc ttctaagcgt gcggaatacc gtgatctggc
      781 aaaaatgtca tttatccccg gtattttcaa tatcaatgaa ccgataatgt ttggccttcc
      841 tattgtactt aaccccatca tgatggtgcc gtttattatg gttcccattg ttaactgtgc
      901 cattggttac ttctttgttt cgatggaaat tattccaccg gttgcttatg ccgtgccctg
      961 gactacgccc ggacctttaa ttgctttcct cggaaccggg gggaactggc tggctttact
     1021 ggttggtttt ttatgtttag gtgtggcgac aatgatctat ttaccttttg ttattgccgc
     1081 caacaaagtc aataacatga caactaacgg ataattccat ttacccagga aggagattgt
     1141 gatgtttgca gatgaagaac ttgttatgga gttactggtc aacgcggggc aggcacgttc
     1201 tgacgcgatg gaagccatac gttgtgccgg gcaaaaagac tggcagggcg caactcagct
     1261 aatggccagt tctgaatctg cctgtcttca ggcacataag attcagaccg cattaattag
     1321 tcaggatgaa ggctgtggca aaataaaagt taatttaatt ctgattcatg cacaggatca
     1381 tttgatgaat gcaatattat gccaggattt agccagggaa attatttcat taagaaaaga
     1441 actccatgcc tgattttttc ctccagtgcg atagctggag gaaattatta tactcttaag
     1501 gataagtcat gaatataaag acgcttaacg tcagcctttt gtctttttct attattacag
     1561 cattgtttcc attgaacgcg atggcaacaa aattaaccat agagcagcgc cttgaattgc
     1621 ttgaaaatga attgtcgcaa aataaacaag agctgaaagc aacacagaat gaactgggag
     1681 tatataaatc ccgactttcg accttacaaa aaagcatcac ggaaaacaaa tataaatcag
     1741 cctcgcttgc tgaaatatca gcgacatctc ccgtcgctga taacatcaaa aatgaaaacg
     1801 gtgaacagaa ctcgtttacc gcagcgcata ctataaatgg atcgcagcaa gttgccgtta
     1861 ttgaaagtaa aggcgacaaa accactatcg aaagcgtgac cctgaaagat atcagtaaat
     1921 atataaagga tgatattggg ttcagctatc aggggtactt ccgctcaggc tggggtactg
     1981 gtaatcacgg ctcaccacaa acttatgcag cgggttctct gggacgtttt ggtaatgaga
     2041 tgagcggttg gtttgacctg accttaaatc agcgtgttta taatcaggac ggtaaaacgg
     2101 caaatgcggt cgttacctat gatggcaacg tgggtgagca gtataacgat gcctggtttg
     2161 gtgacagtgc caatgaaaat atcatgcagt tcagcgatat ttacctgaca acgcgaggtt
     2221 ttttaccctt cgcaccagag gcagacttct gggtcggcaa acataaactc ccgcaatatg
     2281 agatccagat gctggactgg aaaaccttaa ccacggatgt cgccgcgggt gtggggattg
     2341 aaaactgggc acttggtgta gggctgtttg atatgtcctt aagccgtgat gatgtcgatg
     2401 tttactctcg tgatttttcg cgtaccagcc agatgaatac caattctgtg gatgttcgtt
     2461 atcgcaatat cccgttatgg gatgatgcga cattatcatt aatggctaaa tattccgcac
     2521 ctaataaaac ggatcaacaa caagataacg aaaatgacaa cagttatttt gaaatgaaag
     2581 atagctggat gctgacttct gttttacggc aaaacctgca acgcgatacg tttaatgaat
     2641 ttacgttaca ggttgccaat aattcctatg ccagcagttt tgccagtttc tcagatgcca
     2701 gtaatacgat ggcgcatggt cgctattact atggtgatca taccaatggg atcgcctggc
     2761 gtttaatctc tcagggcgag atgtatctta ctgacaatat tattatggct aacgcgcttg
     2821 tctattctca cggcgaagat gtttatagtt atgaaagtgg tgctcatagt gattttgaca
     2881 gtattcgcac tgtaataaga ccggcctgga tctggaatac atggaatcag acggggcttg
     2941 aattaggctg gtttaagcaa cagaacaaaa ctcagcaggg tgtaacacta aatgaatcgg
     3001 cttataaaac gacactctgg catgcattga aagtgggtga aagcatttta ggttcacgac
     3061 cagaaattcg cttctatgga acgtatatca atattctgga taacgaatta tcgaatttta
     3121 agtttaatga gaacagcaaa gacgagttta tggccggcat ccaggcggaa gtctggtggt
     3181 aatctatttt agttaaggag ttgtgtatgt caggatttaa aaaaggtttt ttatggggcg
     3241 gtgcggtagc cgcgcatcag ttggaaggtg gctggaatga aggaggaaaa ggcatcagca
     3301 tcgctgatgt gatgactgct ggcgctcacg gggtgccgcg tgaagtgaca gaaggcgtta
     3361 tcgacgggct taattatccc aatcatgaag caattgattt ttatcatcgc tataaaacag
     3421 atattcagtt atttgccgag atgggattca aatgctttcg aacttccatt gcctggacac
     3481 gaatctttcc gcaaggtgac gaacaggagc cgaatgaaga gggtttacaa ttttatgatg
     3541 atctgttcga tgaatgcctt aagcagggaa tggaacctgt ggtgacgctt tcacattttg
     3601 agatgcctta tcatctggtg acaaaatatg gtggctggcg aaaccgtaaa ctgatcgact
     3661 tttttatccg cttcgcatca acggtcttca cgcgctataa agaaaaagta aagtactgga
     3721 tgacgtttaa cgaaatcaat aatcaggtga atttcagcga aagcctgtgt ccatttacta
     3781 attccggtat cttgtattcg ccagaggaag atatcaatga gcgcgaacaa ataatgtacc
     3841 aggcggtaca ttacgagtta gttgccagtg ccctggcggt acagactgga aaatcgatca
     3901 atcctgaatt taacattggc tgtatgatcg ccatgtgtcc catctatcct ctgacgtgtg
     3961 cacccaacga tatgatgatg gccacgaaag caatgcatcg tcgttactgg tttactgatg
     4021 ttcatgctcg tggatattat ccgcaacata tgctgaatta ctttgccagg aaaggattca
     4081 acctcgatat cacaccagaa gataacgcga ttcttgccag tggttgtgtc gactttatcg
     4141 gctttagcta ctacatgtct tttacgacac aattttcgcc cgataacccg caactggatt
     4201 atgttgaacc acgagatttg gtcagcaacc cttatatcga tacatccgaa tggggatggc
     4261 aaattgatcc ggcagggcta cgttattcac tcaactggtt ctgggatcat ttccagttgc
     4321 cgctgtttat tgtcgaaaat ggatttggtg cggttgacca gagacaagct gacggcacgg
     4381 tgaacgatca ctatcgcatt gattactttg cttcccatat tcgggaaatg aaaaaagccg
     4441 tagttgaaga tggtgttgac ttaattggct acaccccgtg gggctgcatt gacctggttt
     4501 ctgccggaac aggggaaatg aaaaagcgct acggaatgat ttatgtcgat aaagacaacg
     4561 aagggaaggg aacgctggaa cggatacgta aagcgtcgtt ttactagtat cgggatctca
     4621 tcgccaacaa tggcgaaaat atttgagact tgaggaagat attacggaga tgtcaggctc
     4681 tgtgatcaga gcctgacaat aactacatag cgtgttgatt ataatagggc acgccgagcg
     4741 catccgactt atcactgcca gtacccatgt gatttaaatc aaacggtggt tgttcctgcg
     4801 atgggggaac aaccacgaca ttggcacaag aagtaacaac tggctgcgtg gtttgttccg
     4861 caacgccctg cgcggaaaag agaataagcg cagctgctat tgcggatgaa agtggtttca
     4921 tgattgcctc agtgacgttg tcacgttatc aactagctgt tgtaatgatt taatggatgt
     4981 aaaaagcgcg attcgccctg catattggtc atccggtatt tatgcggcgg aacatcaaag
     5041 taatttttga aggttcgggt cagagtttgt tgcgactcga agccatatcg ttctgccaga
     5101 tagagtatcg gctcgttact ttccttcagc ttttgcgcga tttccgtcat cttacggctg
     5161 cggatgtatt ggcctaatga atgaccggtt tcttttttaa acatccgttg caggtgccat
     5221 ttagagtaac ctgaacgctc tgacactttc tccagtgaca gtggcgattc caggttgtcc
     5281 tcgatccagt ccaaaatgct atgaatggta atagcgtcag tattgcgtct ggacatcgtc
     5341 atacctcttt tttgtttacg gcaggacttt cttaagcaaa tgctcaagtg ttgccacttc
     5401 gtccgccgtc aggttttttg ttaattcttg gtgcaggtcc tggccaacta attgatggca
     5461 ttgttcacat attgccgcgc cgctggtggt aagttttacc agtacgccgc gcttatcatt
     5521 cgggttcggc aacctttcaa cccagccttt acagaccagg cgatccagca tacgggtgag
     5581 tgctcccagg tcgaccgaca acactttttt cagttcaacc ggagtaatac acgccgcgca
     5641 gcggatagag cagagcacct taaactgtgc cgcggtaata tccagcggag acagatactc
     5701 gttaagcaga cgatctttct tctgattaac catatggatt aagcgaccca atggaataat
     5761 ttcattgaac agatcgctgg tacttttcac attagttgcc ctggcaagta attagttgca
     5821 ggggataata ttgcccaggc aagtataagt caaatgaatg aattgaccga tgccacgttt
     5881 tgctaaatcg gttcaagttt tggtcaatgc gatgcaaggt tagctaaaga gtgacacttt
     5941 atccttgttt accccttata cttttcgctg ataacccaga tacacaggat aacaaccacc
     6001 aatgttagat ttgtttaaag caattggcct ggggctggtg gtgttgctgc cgttagctaa
     6061 cccattaaca actgtcgcgc tatttcttgg cctggcaggc aacatgaaca gtgccgaacg
     6121 taatcgtcag tcgttgatgg cctcggtgta cgtatttgcc atcatgatgg tggcgtatta
     6181 cgccgggcaa ctggtgatgg atacatttgg catttcgatt cccggtctgc gaattgcagg
     6241 cggcttaatc gtcgcgttta tcggttttcg gatgctcttt ccgcaacaga aagcgattga
     6301 ttcaccggag gcgaaaagca agtcggaaga gctggaagat gaacccagtg ccaatattgc
     6361 ctttgtgccg ctagcaatgc caagtaccgc tggtccggga accatagcga tgattattag
     6421 ctcggcgtcc acagtgcgtc agagttcaac cttcgctgat tgggtactga tggttgcgcc
     6481 gccgctgatc tttttcctgg tggcggttat tttgtgggga agcctacgca gctccggcgc
     6541 aattatgcgg ctggtgggca agggtggcat tgaagcgatc tcccgtctga tgggattcct
     6601 gctggtatgt atgggtgtgc agtttattat taacggcatc ctggaaatca ttaaaacgta
     6661 ccattaaaaa aggcccgaat gggcctttca actattgcgt ctgttcttcg agtgtcactg
     6721 gccagcggcg aaatataatg attgaccaaa ttaacgcggc aaaagcaggc accgcgccca
     6781 cataaccaat catcgacatt gaccagtgca aactcacctg attacctacc aacgcacccg
     6841 ccccgattcc aatattaaat atgccggaga atagcgccat cgcgacgtcg gtagcatctg
     6901 gtgccagcgc cagcacttta acctgcatac caagcccgat gatcatcatc gcgctccccc
     6961 agaaaatact cagcaccccg aggtgtattt cactgttcgc cgcaggtaac agcaatgcca
     7021 ggcacaccag caacagcgca atcgccgtac tcaccaacgc agacgcatac tgattaccca
     7081 gtttaccgaa aatcacgctg ccaataatgc ccgaaccacc gagtaataac agtaatgccg
     7141 tggcaaagtt ggcgctgaat cccgcaatgt tttgtacaaa aggctcgata tagctgtatg
     7201 ccgtgtaatg ggcggtgaca accaccacag ttaacaaata aatgctcatc aatgccgggc
     7261 ggcggaacaa tagcgggagg cttttcagtg aaccggaatg ctcactgggc agtaagggaa
     7321 gtaacttaat caggcacaaa agggtgataa gcgccccaat accaatcgcg aagaaggtca
     7381 ttcgccaacc gaaatactgg cccacaatgc gcccgagagg taaacctaag accatcgcca
     7441 gtgctgtacc ggtggcaatt aaactcaatg cctgtgctcg cttcccggcc ggagccatac
     7501 ggatcgccag agatgccgta atcgaccaga aaatcgcatg tgcaaaagcc acaccaatgc
     7561 gactgatcac cagaacggta aagctccacg acaaaaacga cagtacgtgg ctggcaataa
     7621 acaccacaaa caggcagatc agtaatttgc gccgttcaac ctgactggtc attaacataa
     7681 aaggcaatga cattagcgct actacccatg cgtaaatggt caacatgatg ccgacctgag
     7741 cggtttgcat gtgaaaactt tgcgcaatgt cagagagcag gccaacaggg acaaattcgg
     7801 tggtgttgaa gatgaaggcg gcgactgcca gcgtaacgac ccgtagccac gccactttgc
     7861 gggaaacagt gtttgttgtc ataacagatt atcgggtgag ttgcgaaaaa gtgatgaaat
     7921 gatcttaaaa cggttaatga tgtgaataca atgcatttgt gatctggatc gcgttttccg
     7981 tttctctgac gctgattctg ttccgcgtca gagagtcggt aacatcacgg tttaatgacg
     8041 ttcagggagg tctggcgttt tggcggttgc gggtataggt ggtctgacag gggagcgagg
     8101 cctgtttttc accaggtctt cgtgagtaac ttttggcggc gtttcatttt ttacaaacag
     8161 tttgagggtc ctgcgcgcca tatgtttgat aattttatca tcacttaatg atgctggcat
     8221 caacacgtca ttcagatcac tttcattggg attcataaat tggtgatagt gcaataacat
     8281 attggcgaaa aataaaaaaa gtcctgaaaa tgaggagatt cttgttctgc taaaagggat
     8341 aaaaagcaga tgcagacttc gggttgtaca tgtttgttag aacctaatga atccatcagg
     8401 ctttgcaata aatcctcttt taattcgccg tctgttatgt cgagaatggc ggtgatcaag
     8461 ttaggatctt tattgacgaa ccatctgtga acctcagagt tactccataa tcgggggagt
     8521 atgacaaaat taccgcgaga gataaaattc aggacaagca aatcaaactg gcgtaagtaa
     8581 tctgcagcgg tatggcgatc cggaggcagg ggttcaatat gtggtgtatg cgctcttttg
     8641 atgggaacgc caaggttgat cattggaaat tgttcaataa aatcattcag aaacagaaat
     8701 agcacacgct cgacgccggt ctgaaatacg cgc
//
LOCUS       DAFNBJ010000186         8678 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000186 DAFNBJ010000000
VERSION     DAFNBJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.186"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(285..548)
                     /gene="rpsT"
                     /locus_tag="L2X49_004239"
     CDS             complement(285..548)
                     /gene="rpsT"
                     /locus_tag="L2X49_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBN4799234.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            651..869
                     /locus_tag="L2X49_004240"
     CDS             651..869
                     /locus_tag="L2X49_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBN4799235.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            877..1818
                     /gene="ribF"
                     /locus_tag="L2X49_004241"
     CDS             877..1818
                     /gene="ribF"
                     /locus_tag="L2X49_004241"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBN4799236.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1861..4677
                     /gene="ileS"
                     /locus_tag="L2X49_004242"
     CDS             1861..4677
                     /gene="ileS"
                     /locus_tag="L2X49_004242"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBN4799237.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4677..5171
                     /gene="lspA"
                     /locus_tag="L2X49_004243"
     CDS             4677..5171
                     /gene="lspA"
                     /locus_tag="L2X49_004243"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBN4799238.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5296..5745
                     /gene="fkpB"
                     /locus_tag="L2X49_004244"
     CDS             5296..5745
                     /gene="fkpB"
                     /locus_tag="L2X49_004244"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBN4799239.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5747..6697
                     /gene="ispH"
                     /locus_tag="L2X49_004245"
                     /gene_synonym="lytB"
     CDS             5747..6697
                     /gene="ispH"
                     /locus_tag="L2X49_004245"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBN4799240.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCIGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6763..7677
                     /gene="rihC"
                     /locus_tag="L2X49_004246"
     CDS             6763..7677
                     /gene="rihC"
                     /locus_tag="L2X49_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBN4799241.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7844..8665
                     /gene="dapB"
                     /locus_tag="L2X49_004247"
     CDS             7844..8665
                     /gene="dapB"
                     /locus_tag="L2X49_004247"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBN4799242.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKEGGLFDMRDVLDLNSL"
BASE COUNT         1976 a         2164 c         2473 g         2065 t
ORIGIN      
        1 agagtggtct taagtttaat acctcaatgt gtatcacaat atccacattc tttgtagggg
       61 agtctggaga ttgagtagat attcttgttc agaatgtatc agccgatggt tctacgattc
      121 ttaagccacg aagagttcag aaagtgtaac ggcatgtctg caaaagccag catcttttga
      181 ctatctggca accggtagtg tgttctctca cgcatcataa aagcagcggg cataaaaaaa
      241 cccgcttgcg cgggcttttt cacaaagctt cagcaaattg gcgattaagc cagtttgttg
      301 atctgtgcag tcaggttagc cttatgacgt gcagctttgt ttttgtggat cagaccttta
      361 gcagcctgac ggtccacgat cggttgcatt tcgttaaatg ctttctgtgc agcagctttg
      421 tcgccagctt cgatagctgc gtatactttc ttgatgaaag tacgcatcat agagcgacgg
      481 cttgcgttgt gcttacgagc cttttcagac tgaatggcgc gcttcttagc tgatttgata
      541 ttagccaagg tccaactccc aaatgtgttc tatatggaca attcaaaggc cgaggaatat
      601 gcccttttag ccttcttttg tcaatggatt tgtgcaaata agcgccgtta atgtgccggc
      661 actcgttacg tagtgatggc gcaggattct accagcttgc ggggtgtgaa tacagctttt
      721 ccgcgataaa aattgcagca ggcggtcact ttcttcccgt gatttgcgcc atggcaatga
      781 aaagccactt ctttctgatt tcggtactca atcgccggtt aaccttgacc gctgtacaag
      841 gtatactcgg acgattttca ctgttttgag ccagacatga agctgatacg cggcatacat
      901 aatctcagcc aggccccgca agaagggtgt gtgctgacta ttggtaattt cgacggcgtg
      961 catcgcggtc atcgcgcgct gttacagggc ttgcaggaag aagggcgcaa gcgcaactta
     1021 ccggtgatgg tgatgctttt tgaacctcaa ccactggaac tgtttgctac cgataaagcc
     1081 ccggcaagac tgacccggct gcgggaaaaa ctgcgttacc ttgcagagtg tggcgttgat
     1141 tacgtgctgt gcgtgcgttt cgacaggcgt ttcgcggcgt taaccgcgca aaatttcatc
     1201 agcgatcttc tggtgaagca tttgcgcgta aaatttcttg ccgtagggga tgatttccgc
     1261 tttggcgctg gtcgtgaagg cgatttcttg ttattacaga aagctggcat ggaatacggc
     1321 ttcgatatca ctagtacgca aactttttgc gaaggtggcg tgcgtattag cagcaccgct
     1381 gtgcgtcagg cgctggcgga tgacaatctg gctctggcag aaagtttact ggggcacccg
     1441 tttgccatct ccgggcgtgt agtccacggt gatgaattag ggcgcactat aggtttcccg
     1501 acggcgaatg taccgctacg ccgtcaggtt tccccggtga aaggggttta tgcggtagaa
     1561 gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa acatcggaac acgcccaacg
     1621 gttgccggta ttcgccagca gctggaagtg catttgttag atgttgcaat ggacctttac
     1681 ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca atgagcagcg atttgcgtcg
     1741 ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa ccgcccgcga attttttggg
     1801 ctaacaaaac cggcttaagc ctgttatgta atcaaaccga aacacggaac cgagaatctg
     1861 atgagtgact ataaatcaac cctgaatttg ccggaaacag ggttcccgat gcgtggcgat
     1921 ctcgccaagc gcgaaccggg aatgctggcg cgttggactg atgatgatct gtacggcatc
     1981 atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc atgatggccc tccttatgcg
     2041 aatggcagca ttcatattgg tcactcggtt aacaagattc tgaaagacat tatcgtgaag
     2101 tccaaagggc tttccggtta tgactcgccg tatgtgcctg gctgggactg ccacggtctg
     2161 ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg gtgagaaatt caccgccgcc
     2221 gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg ttgacggtca acgcaaagac
     2281 tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt acctgaccat ggacttcaaa
     2341 actgaagcca acatcatccg cgcgctgggc aaaatcatcg gcaacggtca cctgcacaaa
     2401 ggcgcgaagc cggtacactg gtgcgttgac tgccgttctg cgctggcgga agcggaagtt
     2461 gagtattacg acaaaacttc tccgtccatc gacgttgctt ttcaggcggt cgatcaggat
     2521 gcactgaaag caaaatttgc cgtaagcaac gttaacggcc caatctcgct ggtgatctgg
     2581 accactacgc cgtggactct gcctgcgaac cgcgcaatct ctattgcacc tgatttcgac
     2641 tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg cgaaagatct ggttgaaagc
     2701 gtaatgcagc gtatcggcgt gaccgattac accattctcg gcacggtaaa aggtgcggag
     2761 cttgagttgc tgcgctttac ccatccgttt atgggcttcg acgttccggc aatcctcggc
     2821 gatcacgtta ccctggatgc cggtaccggt gccgttcaca ccgcgcctgg ccacggcccg
     2881 gacgactatg tgatcggtca gaaatacggc ctggaaaccg ctaacccggt tggcccggac
     2941 ggcacttatc tgccgggcac ttatccgacg ctggatggcg tgaacgtctt caaagcgaac
     3001 gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc tgcacgttga gaaaatgcag
     3061 cacagctatc cgtgctgctg gcgtcacaaa acgccgatca tcttccgcgc gacgccgcag
     3121 tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt cactgaaaga gatcaaaggc
     3181 gtgcagtgga tcccggactg gggccaggcg cgtatcgagt cgatggttgc taaccgtcct
     3241 gactggtgta tctcccgtca gcgcacctgg ggcgtaccga tgtcactgtt cgtgcacaaa
     3301 gacacggaag agctgcatcc gcgtactctc gaactaatgg aagaagtggc aaaacgcgtt
     3361 gaagttgacg gcatccaggc gtggtgggat cttgatgcga aagagatcct cggcgatgaa
     3421 gctgatcagt acgtgaaagt gccggacaca ttggatgtat ggtttgactc cggatctacc
     3481 cactcttctg ttgttgacgt gcgtccggaa tttgccggtc acgcagcgga catgtatctg
     3541 gaaggttctg accagcaccg tggttggttc atgtcttccc taatgatctc caccgcgatg
     3601 aaaggcaaag cgccgtatcg tcaggtactg acccacggct ttaccgtgga tggtcagggc
     3661 cgcaagatgt ctaaatccat cggcaatacc gtttcgccgc aggatgtgat gaacaaactg
     3721 ggcgcggata ttctgcgtct gtgggtggca tcaaccgact acaccggtga aatggccgtt
     3781 tctgacgaga tcctgaaacg tgctgccgat agctatcgtc gtatccgtaa caccgcgcgc
     3841 ttcctgctgg caaacctgaa cggttttgat ccagcaaaag atatggtgaa accggaagag
     3901 atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag cggcacagga agacatcctc
     3961 aaggcgtacg aagcatacga tttccacgaa gtggtacagc gtctgatgcg cttctgctcc
     4021 gttgagatgg gttccttcta cctcgacatc atcaaagacc gtcagtacac cgccaaagcg
     4081 gacagtgtgg cgcgtcgtag ctgccagact gcgctgtatc acatcgcaga agcgctggtg
     4141 cgctggatgg caccaatcct ctccttcacc gctgatgaag tgtggggcta cctgccgggc
     4201 gaacgtgaaa aatacgtctt caccggtgag tggtacgaag gcctgtttgg cctggcagac
     4261 agtgaagcga tgaacgatgc gttctgggac gagctgttga aagtgcgtgg cgaagtgaac
     4321 aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg gctcgctgga agcggcagta
     4381 accttgtatg cagaaccgga actgtcggcg aaactgaccg cgctgggcga tgaattacga
     4441 tttgtcctgt tgacctccgg cgctaccgtt gcagactata acgacgcacc tgctgatgct
     4501 cagcagagcg aagtactcaa agggctgaaa gtcgcgttga gtaaagccga aggtgagaag
     4561 tgcccacgct gctggcacta cacccaggat gtcggcaagg tggcggaaca cgcagaaatc
     4621 tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa aacgtaagtt tgcctgatga
     4681 gtcaatcgat ctgttcaaca gggctacgct ggctgtggct ggtggtagtc gtgctgatta
     4741 tcgatctggg cagcaaatac ctgatcctcc agaactttgc tctgggggat acggtcccgc
     4801 tgttcccgtc gcttaatctg cattatgcgc gtaactatgg cgcggcgttt agtttccttg
     4861 ccgatagcgg cggttggcag cgttggttct ttgccggtat tgcgattggt attagcgtga
     4921 tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa gctaaacaat atcgcttacg
     4981 cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg cctgtggcac ggcttcgttg
     5041 tcgatatgat cgacttctac gtcggcgact ggcacttcgc caccttcaac cttgccgata
     5101 ctgccatttg tgtcggtgcg gcactgattg tgctggaagg ttttttacct tctaaagcga
     5161 aaaaacaata ataaaccctg ccggatgcga tgctgacgca tcttatccgg cctacagatt
     5221 gctgcgaaat cgtaggccgg ataaggcgtt tacgccgcat ccggcaaaaa tccttaaata
     5281 taagagcaaa cctgcatgtc tgaatctgta cagagcaata gcgccgtcct ggtgcacttc
     5341 acgctaaaac tcgacgatgg caccaccgcc gagtctaccc gcaacaacgg taaaccggcg
     5401 ctgttccgcc tgggtgatgc ttctctttct gaagggctgg agcaacacct gttggggctg
     5461 aaagtgggcg ataaaaccac cttctcgttg gagccagatg cggcgtttgg cgtgccgtca
     5521 ccggacctga ttcagtactt ctcccgccgt gaatttatgg atgcaggcga gccagaaatt
     5581 ggcgcaatca tgctttttac cgcaatggat ggcagtgaga tgcctggcgt gatccgcgaa
     5641 attaacggcg actccattac cgttgatttc aaccatccgc tggccgggca gaccgttcat
     5701 tttgatattg aagtgctgga aatcgatccg gcactggagg cgtaacatgc agatcctgtt
     5761 ggccaacccg cgtggttttt gtgccggggt agaccgcgct atcagcattg ttgaaaacgc
     5821 gctggccatt tacggcgcac cgatatatgt ccgtcacgaa gtggtacata accgctatgt
     5881 ggtcgatagc ttgcgtgagc gtggggctat ctttattgag cagattagcg aagtaccgga
     5941 cggcgcgatc ctgattttct ccgcacacgg tgtttctcag gcggtacgta acgaagcaaa
     6001 aagccgcgat ttgacggtgt ttgatgccac ctgtccgctg gtgaccaaag tgcatatgga
     6061 agtcgcccgc gctagtcgcc gtggcgaaga atctattctc atcggtcacg ccgggcaccc
     6121 ggaagtggaa ggcacgatgg gtcagtacag caacccggaa gggggaatgt atctggttga
     6181 atcaccagac gatgtgtgga aactgacggt caaaaacgaa gagaaactct cctttatgac
     6241 ccagaccacg ctgtcggtgg atgacacgtc tgatgtgatc gacgcgctgc gtaaacgctt
     6301 cccgaaaatt gtcggtccgc gcaaagatga catctgctac gccacgacta accgacagga
     6361 agcggtacgt gccctggccg aacaggcgga agttgtgctg gtggtcggtt cgaaaaactc
     6421 ctctaactcc aaccgtctgg cggagctggc ccagcgtatg ggcaaaagcg cgtttttgat
     6481 tgatgatgcg aaagatatcc aggaagagtg ggtgaaagag gttaaatgca tcggcgtgac
     6541 tgcgggcgca tcggctccgg atattctggt gcagaatgtg gtggcacgtt tgcaacagct
     6601 gggcggtggt gaagccattc cgctggaagg acgtgaagaa aacattgttt tcgaagtgcc
     6661 gaaagagctg cgtgtcgata ttcgtgaagt cgattaagtc attagcagcc taagttatgc
     6721 gaaaatgccg gtcttgttac cggcattttt tatggagaaa acatgcgttt acctatcttc
     6781 ctcgatactg accccggcat tgacgatgcc gtcgccattg ccgccgcgat ttttgcaccc
     6841 gaactcgacc tgcaactgat gaccaccgtc gcgggtaatg tctcggttga gaaaactacc
     6901 cgcaatgccc tgcaactgct gcatttctgg aatgcggaga ttccgctcgc ccaaggggcc
     6961 gctgtgccgc tggtacgcgc accgcgtgat gcggcatctg tgcacggcga atcgggaatg
     7021 gctggctacg actttgttga gcacaaccga aagccgctcg ggataccggc gtttctggcg
     7081 attcgggatg ccctgatgcg tgcaccagag cctgttaccc tggtggccat tggcccgtta
     7141 accaatattg cgctgttact ttcacaatgc ccggaatgca agccgtatat tcgccgtctg
     7201 gtgatcatgg gtggttctgc cggacgcggc aactgtacgc caaacgccga gtttaatatc
     7261 gctgccgatc cagaagcggc tgcctgtgtc ttccgtagcg gaatcgaaat cgtcatgtgc
     7321 ggtctggatg tcaccaatca ggcaatatta acccctgact atctcgctac tttgccggag
     7381 ttgaatcgca ccggaaaaat gcttcatgca ctgtttagcc actaccgtag cggcagtatg
     7441 caaagcggtc tgcgaatgca cgatctctgc gccatcgcct ggctggtgcg cccggactta
     7501 ttcaccctca agccgtgttt tgtcgcggta gaaactcaag gcgaatttac ctcaggcacg
     7561 acggtggttg atatcgacgg ttgcctgggc aaaccagcca atgtaaaggt ggcattggat
     7621 ctggatgtga aaggcttcca gcagtgggtg gctgaggtgc tggctctggc gtcgtaacct
     7681 gtcacatgtt atcggcatgc agtcattcat cgactcatgc ctttcactga tatccctccc
     7741 tgtttatcat taatttctaa ttatcagcgt ttttggctgg cggcgtagcg atgcgctggt
     7801 tactctgaaa acggtctatg caaattaaca aaagagaata gctatgcatg atgcaaacat
     7861 ccgcgttgcc atcgcgggag ccggggggcg tatgggccgc cagttgattc aggcggcgct
     7921 ggcattagaa ggcgtgcagc tgggcgcggc gctggagcgc gaaggatctt ctttactggg
     7981 cagcgacgcc ggtgagctgg ctggtgcagg gaaaacaggc gttaccgtgc aaagcagcct
     8041 cgatgcggta aaagatgatt ttgatgtgtt tatcgatttt acccgtccgg aaggtacgct
     8101 gaaccatctc gctttttgtc gccagcatgg caaagggatg gtgatcggca ctacggggtt
     8161 tgacgaagcc ggtaaacagg cgattcgtga cgccgccgcc gatattgcga ttgtctttgc
     8221 cgccaatttt agcgttggcg ttaacgtcat gcttaagctg ctggagaaag cagccaaagt
     8281 gatgggtgac tacaccgata tcgaaattat tgaagcacat catagacata aagttgatgc
     8341 gccgtcaggc accgcactgg caatgggaga ggcgatcgcc cacgcccttg ataaagatct
     8401 gaaagattgc gcggtctaca gtcgtgaagg ccacaccggt gaacgtgtgc ctggcaccat
     8461 tggttttgcc accgtgcgtg caggtgacat cgttggcgaa cataccgcga tgtttgccga
     8521 tattggcgag cgtctggaga tcacccataa ggcgtccagc cgcatgacat ttgctaacgg
     8581 cgcggtaaga tcggctttgt ggttgagtgg taaggaaggt ggtctttttg atatgcgaga
     8641 tgtgcttgat ctcaatagtt tgtaaccaca aaatattt
//
LOCUS       DAFNBJ010000187         8582 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000187 DAFNBJ010000000
VERSION     DAFNBJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.187"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..76
                     /locus_tag="L2X49_004248"
     CDS             <1..76
                     /locus_tag="L2X49_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HBN4799243.1"
                     /translation="NVESRADTALMEARTKDILALLNQ"
     gene            158..1552
                     /gene="wcaJ"
                     /locus_tag="L2X49_004249"
     CDS             158..1552
                     /gene="wcaJ"
                     /locus_tag="L2X49_004249"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBN4799244.1"
                     /translation="MTNLKKRERARTNASLISMVQRFSDITIMVGGLWAVCWISGQSF
                     LYMHLLMALIALVVFQMIGGMTDFYRSWRGVKMTTELMLLLQNWTLSLIFSAGLVAFS
                     HDFDNRLVTYLCWYLLTSVGMVVCRSLIRFGAGWLRNRGYNRRYVAVAGDLPVGQVLL
                     DSFRKEPWLGFEVVGSYHDAKPGGVPSDWAGNYDQLIDDAKAGKIHNVYIAMQMKEES
                     RIKKLMRDLADTTCSVILIPDVFTFNILHSRIEEVNGVPVVPLYDTPLSGINRVLKRL
                     EDIVLSSLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIMVWKFRSMKVME
                     NDKVVTQATQNDPRVTRVGNFLRRTSLDELPQFINVFTGGMSIVGPRPHAVAHNEQYR
                     SLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRIEFDLEYIREWSLWFDIKIV
                     FLTIFKGFVSKAAY"
     gene            1554..3032
                     /gene="wzxC"
                     /locus_tag="L2X49_004250"
     CDS             1554..3032
                     /gene="wzxC"
                     /locus_tag="L2X49_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBN4799245.1"
                     /translation="MSLREKTISGAKWSAMATIVIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISQLELTTLYWLNVGLGISVFVLVFLLSDVI
                     ASVLHNPDLAPLMRTLSFAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVSSNLRFGAWLTA
                     DSIINYVNTNLSTLVLARILGASVTGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPVLLGLMVVSSNFVPLVFGEKWNSIIPILQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIIVGGHMAGAIGVTLGFLLVQI
                     INTVLSYFIMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYGLGVLLKGHLPLAALLA
                     VQVAAGALAFGVMIVLSRNALVVEMKRQFCRNEKMKTLLRAG"
     gene            3050..4330
                     /gene="wcaK"
                     /locus_tag="L2X49_004251"
     CDS             3050..4330
                     /gene="wcaK"
                     /locus_tag="L2X49_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBN4799246.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINTLKPETEVDVMSRYPV
                     SSSWLLNRPVMGDPLYSQMKQHNNAAGVMGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQEPQFNQLANYVFGHCDALILRESVSLDMMKRSEIDTSKVEHGVDTAWLVDHQDDSF
                     QPSYAVQHWLDVAAKQKTVAITLRELAPFDKRLGTTQAAYEKAFAEVVNRVLDSGYQV
                     LALSTCTGIDSYNKDDRMVALNLRHLVNDPSRYHVVMDELNDLEMGKLLSACDLTVGT
                     RLHSAIISMNFGTPAIAINYEHKSAGIMQQLGMPEMAVDIRHLLDGSLGAMVGDTLGQ
                     LPAVNERLAVVVKAEREKGMGMVKSVLDRVREGK"
     gene            4327..5547
                     /gene="wcaL"
                     /locus_tag="L2X49_004252"
     CDS             4327..5547
                     /gene="wcaL"
                     /locus_tag="L2X49_004252"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBN4799247.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGYDVEIIALQKGDTQN
                     THAAYTQYGLEAKTRWLQDEPSGKVNKLRYRASQTLRGLHRASTWRALNISRYGAESR
                     NLILSAMCGQTAQPYRADVFIAHFGPAGVTAAKLRELGVIDGKIATIFHGIDISSREV
                     LNHYTPEYQQLFQRGDMMLPISDLWAGRLKKMGCPGEKITVSRMGVDLARFTRRPVKV
                     PGKPLQIISVARLTEKKGLHVAIEACRQLKARGVDFRYRILGIGPWERRLRTLIEQYE
                     LETHVEMPGFKPSHEVKAMLDEADVFLLPSVTGADGNMEGIPVALMEAMAVGIPVVST
                     LHSGIPELIKSDHSGWLVPENNAMALADRLAAFSDIDQQALEPVLHNARQKVEADFNQ
                     QVINRKLASLLQTL"
     gene            5560..6951
                     /gene="wcaM"
                     /locus_tag="L2X49_004253"
     CDS             5560..6951
                     /gene="wcaM"
                     /locus_tag="L2X49_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBN4799248.1"
                     /translation="MLKKITRRSFVSSLSVLAAMPLLSPRVARAATGKTVSVNQYNNN
                     DWIAAFKQAFNEGDTVVVPAGLTCKDINTGIFIPDGKTLLIRGALSGNGWGRFVLQEG
                     SKVIGEGEGRTENITLDVRGSDCVIKGLAMSGFGPVTQIYIGGKKPSVMRNLVIDNIR
                     VSQANYAILRQGFHNQVDGARITNSKFSHLQGDAIEWNVAINDRNILISDHVIDNINC
                     TNGKINWGIGIGLAGSTYDNDYPEKQTVKNFVVANITGSNCRQLVHVENGKHFIIRNI
                     KAKNITPDFSKKAGIDNATVAIYGCDNFVIDNVDMVNSAGMLIGYGVIKGDYLSIPQN
                     FKLNDIRLDNSQLAYKLRGIQISSGNATSFVAITNVDMQRATLELHNKPQHLFLRNIN
                     VMQEAAIGPALKLNFDLRKDVRGKFMAKDETLLSLANIKAVNEKGQSSVDIDRVDQHV
                     VNTERLNFALPHR"
     gene            7127..8023
                     /gene="galF"
                     /locus_tag="L2X49_004254"
     CDS             7127..8023
                     /gene="galF"
                     /locus_tag="L2X49_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GalU regulator GalF"
                     /protein_id="HBN4799249.1"
                     /translation="MINLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEIVLVTHSSKNAVENHFDTSYELEALLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQAQPLGLGHSILCARPVVGDNPFIVVLPDIIIDTASADPLRYNLAAMVARFNETGRS
                     QVLAKRMKGDLSEYSIIQTKEALETEGQVSRIVEFIEKPDQPQTLDSDLMAVGRYVLN
                     ADIWAELEKTEPGAWDRIQLTDAIAELAKKQSVDAMLMTGESYDCGKKLGYMQAFVNY
                     GLRNLKEGAKFRSRIEKLLAND"
     gene            8341..>8582
                     /locus_tag="L2X49_004255"
     CDS             8341..>8582
                     /locus_tag="L2X49_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015741779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HBN4799250.1"
                     /translation="MKRIITFGTFDVFHVGHVNILERAASLGDYLIVGVSSDKLNFNK
                     KGRYPIYNQEDRCRIINSLRVVNDVFIEESLEQKKE"
BASE COUNT         1940 a         2169 c         2465 g         2008 t
ORIGIN      
        1 gaacgtggaa tcccgcgccg acaccgcgct gatggaagcc cgaacgaagg acattctggc
       61 tctgttgaat cagtaatcat tgccgctcac cccagccctc tccccacagg ggagagggtt
      121 agggtgaggg gtaaggtttt gaactaaagg aacaacgatg acgaatctaa aaaagcgcga
      181 acgagcgaga acgaatgcat cgttaatctc tatggtgcag cgtttttctg atatcaccat
      241 catggtcggc ggattgtggg cggtgtgttg gatcagcggg caatcgttct tatacatgca
      301 cctgctgatg gcactgattg cgctggtcgt gtttcagatg atcggcggga tgacggattt
      361 ctatcgttcg tggcgtggcg tcaagatgac caccgaactg atgctgttgc tgcagaactg
      421 gaccttaagc ctgatcttca gcgcgggcct ggtggcgttc agccatgatt ttgataatcg
      481 tctggtgacg tatctttgct ggtatctgtt aaccagcgtc ggcatggtgg tttgccgctc
      541 ccttatccgc tttggcgcgg gctggttgcg taaccgtggt tacaaccgcc gctatgtcgc
      601 cgttgccggc gatctgcctg tgggccaggt gctgcttgat agcttccgca aagagccgtg
      661 gttaggtttt gaagtggttg ggagctacca cgacgcgaaa ccgggtggcg taccgtcgga
      721 ctgggcgggc aactatgacc agcttattga cgatgcgaaa gcgggaaaaa ttcacaacgt
      781 ctacattgcg atgcagatga aagaggagtc ccgcattaag aaactgatgc gcgacctggc
      841 ggataccacc tgttccgtga tcctcattcc ggacgtcttt accttcaaca ttctccattc
      901 ccgtatcgaa gaggtgaacg gtgtaccggt agtgccgctg tacgacacgc cgctgtcagg
      961 tatcaaccgt gttctcaaac gcctggagga cattgtgctc tcttcactga tccttctgct
     1021 gatctccccg gtgctgtgct gtattgccct ggcggtgaag ctgagctctc ccgggccagt
     1081 tatcttccgt cagacgcgct acggcatgga cggtaagccg attatggtgt ggaaattccg
     1141 ctccatgaag gtgatggaga acgacaaagt ggtgacgcag gctacgcaga acgatccgcg
     1201 cgtgacccgc gtggggaatt ttctgcgccg cacctcgctg gacgagctgc cgcagtttat
     1261 caacgtcttt accggcggta tgtcgattgt cgggccacgt ccgcatgcgg ttgcccataa
     1321 cgagcagtac cgttcgctga ttgaaggcta catgctgcgc cataaagtga agccaggcat
     1381 taccggctgg gcgcaaatca acggctggcg cggtgaaacc gacacgctgg aaaaaatgga
     1441 aaaacgcatt gaattcgacc ttgagtacat ccgtgagtgg agcctctggt tcgatatcaa
     1501 aattgttttt ctgaccatct tcaaaggttt cgtgagcaaa gcggcgtact aagatgagct
     1561 tacgtgaaaa aaccatcagc ggcgccaagt ggtcagcgat ggcgactata gtcatcattg
     1621 gcctggggct ggtgcagatg accgtgctgg cgcgcattat cgataatcat cagttcggtc
     1681 tgctgaccgt ctcgctggtg attatcgcgc tggccgacac gctgtctgac ttcggtatcg
     1741 ccaactcgat tatccagcgc aaagagatta gccagctgga gctgaccacg ctctactggc
     1801 tgaacgtggg gctggggata tctgtttttg tgctggtgtt cctgctgagc gatgtgatcg
     1861 ccagcgtgct gcacaacccg gatctggccc cgctgatgcg cacgctgtcg tttgcctttg
     1921 tggtgatccc gcacgggcag cagttccgcg cgctgatgca aaaagagctg gagttcaaca
     1981 agatcggcat gattgaaacc agcgccgtgc tggcgggctt taccttcacg gtggtgagcg
     2041 cccatttctg gccgctggcc atgaccgcca ttctggggta tctggtgaac tcagccgtgc
     2101 gcacgctgct gttcggctac tttggtcgca agatctaccg cccggggctg catttctccc
     2161 tggcgtccgt ctcctcgaac ctgcgttttg gcgcttggct gacggccgac agcatcatca
     2221 actatgtcaa caccaacctt tccacgctgg tgctggcgcg cattctgggg gcgagcgtca
     2281 cagggggcta taacctggcc tacaacgttg cggtcgtccc gccgatgaag ctcaacccga
     2341 ttatcacccg cgtgttgttc ccggcgttcg ccaaaattca ggacgacact gaaaagctgc
     2401 gcgtcaactt ctacaagctg ctctccgtgg tcggcattat taacttcccg gtactgctgg
     2461 gtctgatggt ggtgtcgagc aactttgtgc cgctggtgtt tggcgagaag tggaacagca
     2521 tcatcccgat cctgcagttg ctctgcgtgg tggggctgct gcgctccgtg ggtaacccga
     2581 ttggctccct gctgatggcg aaagcgcgcg tggatatcag ctttaaattc aacgtattta
     2641 aaaccttcct gtttattccg gcaattatcg tcggcggaca catggcgggc gccattggcg
     2701 tcacgctggg ctttctgctg gtgcaaatca tcaacaccgt gctgagctac ttcatcatga
     2761 tcaagccagt gctgggctcc agctaccgtc agtacatcct gagcctgtgg ctgccgtttt
     2821 atctctcctt gccgaccctg gtggtgagct acggtctggg cgtgctcctg aagggccatt
     2881 tgccgctggc cgcgctgctg gcagtgcagg ttgccgcggg cgcactggcg tttggggtga
     2941 tgattgtgct gtcacgcaat gcgctggtgg tggagatgaa gcgccagttt tgccgtaacg
     3001 aaaaaatgaa aacgctgctt cgcgcaggct agttatttaa gaggccatta tgaaattatt
     3061 aattcttggc aaccatacct gcggcaaccg tggcgacagc gccatcctgc gcggtttatt
     3121 ggatgcaatt aacaccctta agcctgagac agaagtggac gtgatgagcc gttacccggt
     3181 cagctcatcc tggttactga accgcccggt gatgggcgac ccgctctaca gccagatgaa
     3241 acagcacaat aacgctgcgg gcgtgatggg acgcgtgaag aaagtgttgc gtcgccgcta
     3301 ccagcatcag gtactgcttt ctcgcgtaac cgacaccggc aagctgcgca acatcgccat
     3361 cgcgcagggc tttaccgatt ttgtgcgttt gctttccggc tatgacgcca tcattcaggt
     3421 gggcggttcg ttctttgtcg atctctacgg cgtgccgcag tttgagcatg cgctctgtac
     3481 ctttatggcg aagaagccgc tgtttatgat tggtcacagc gtcgggccat tccaggaacc
     3541 tcagttcaac cagctggcaa actatgtgtt tggccactgc gatgccctga ttttacgtga
     3601 gtcagtcagc ctcgacatga tgaagcgcag cgagatcgac acttcgaaag tcgagcacgg
     3661 ggtggatacc gcgtggctgg tggatcacca ggatgacagc ttccagccga gctacgcggt
     3721 gcagcactgg ctggacgtgg cggcgaaaca gaaaaccgtc gccattaccc tgcgcgagct
     3781 ggcacctttt gataagcgtt taggtacgac tcaggccgcc tacgagaagg cctttgccga
     3841 ggtcgttaac cgcgtgctgg acagcggtta tcaggtgctg gcgctctcta cctgtaccgg
     3901 tatcgatagc tacaacaagg atgatcgcat ggtggcgctg aacctgcgcc atctggtgaa
     3961 cgatccgtcc cggtatcacg tggtgatgga cgaactgaac gatctggaga tgggcaaact
     4021 gctctctgcc tgtgacctga ccgtgggcac ccgtctgcac tctgccatca tctcgatgaa
     4081 cttcggcacg ccggccattg ccatcaacta tgagcacaaa tctgccggga ttatgcagca
     4141 gctggggatg ccggaaatgg ccgtggatat ccgtcatctg ttggatggct cgctcggcgc
     4201 aatggtgggc gacacccttg ggcaactgcc cgccgtgaat gagcgcctgg ccgtggtggt
     4261 gaaagccgag cgcgaaaagg gaatggggat ggtgaagtcg gtgcttgacc gcgtgcggga
     4321 ggggaaatga aggttggttt cttcttactg aaatttccac tgtcgtctga aacctttgta
     4381 ctcaatcaaa tcaccgcgtt tatcgatatg ggatatgacg tggaaattat cgccctgcaa
     4441 aagggcgata cgcaaaacac ccatgcggcg tatacccagt atgggctgga ggcgaaaacc
     4501 cgctggctgc aggatgagcc atccggaaaa gtgaacaagc tgcgctaccg tgccagccag
     4561 accttacgcg ggcttcatcg cgcgtcgacc tggcgagcgc tgaatatctc ccgctatggc
     4621 gcggagtcgc gcaatttgat cctgtcggcc atgtgtgggc aaacggcgca gccgtaccgc
     4681 gctgatgtct ttatcgccca ctttggcccg gcaggggtga ccgcagccaa actgcgcgag
     4741 ctgggggtga ttgacgggaa gatcgccacg atcttccacg gcattgatat ctccagccgt
     4801 gaagtgctga accactacac gccggagtat cagcagctgt tccagcgcgg cgacatgatg
     4861 ctgcccatca gcgacctgtg ggccggacgc ctcaaaaaga tgggctgccc gggcgagaaa
     4921 atcaccgtct cgcggatggg tgtggatttg gcgcgtttta cccgccgtcc ggtcaaggtg
     4981 ccgggtaaac cgctgcagat tatctccgtt gcccgactga ccgagaaaaa agggctgcat
     5041 gtggcgattg aagcctgccg ccagctcaag gcgcgcgggg tggatttccg ttatcgcatt
     5101 ctcggtatcg gcccgtggga gcgtcgtctg cgtacgctta ttgaacagta tgagctggaa
     5161 acgcacgtgg agatgccggg ctttaagccg agccacgagg tgaaggccat gctcgatgaa
     5221 gccgacgtgt ttctgctgcc gtccgttact ggcgccgatg gcaacatgga aggcataccg
     5281 gtggcgctga tggaggcgat ggcggtgggg attccggtgg tatcgacgct gcacagcggg
     5341 atcccggagc tgatcaagtc agatcattcc ggctggctgg tccccgaaaa taacgccatg
     5401 gccctggccg atcggctggc cgcgtttagc gatatcgacc agcaggcgct ggagcccgtg
     5461 ctgcacaatg cccgacaaaa agtcgaggca gattttaacc agcaggtgat caatcgcaag
     5521 ttagcgagcc tgctgcaaac gttgtaacgc cgaggttgta tgctgaaaaa gattacccga
     5581 cgcagctttg tctcttctct gtccgtcctg gcggcaatgc cgctactgtc gccccgcgtt
     5641 gcgcgggcgg caaccggcaa gacggtctct gtaaatcagt acaataacaa cgactggatc
     5701 gccgccttta agcaggcgtt taacgaaggc gacaccgtcg tagtgcctgc agggctcacc
     5761 tgcaaagaca tcaacacggg cattttcatt cctgacggca aaacgctgct gattcgcggg
     5821 gcgttaagcg gcaatggctg ggggcgcttt gtcctgcagg agggcagcaa agtcatcggt
     5881 gagggcgaag gccgtaccga aaatattacc cttgacgttc gtggctctga ctgtgtcatc
     5941 aaagggctgg caatgagcgg atttggcccg gtaacgcaaa tctacattgg cggcaaaaag
     6001 cccagcgtga tgcgtaatct ggtcattgat aacatccgcg tgagccaggc caattacgcc
     6061 atcctgcgtc aggggtttca taaccaggtg gatggcgccc gcatcaccaa cagcaaattc
     6121 agccacctgc agggcgatgc gatagagtgg aatgtggcca tcaatgaccg caatattctg
     6181 atctccgacc atgtgatcga caacatcaac tgcaccaacg ggaagattaa ctgggggatt
     6241 ggtattggcc ttgcgggcag tacctacgac aacgactacc cggaaaaaca gaccgtgaag
     6301 aactttgtgg ttgccaatat caccggcagc aactgccgcc agctggtgca cgttgagaac
     6361 ggtaaacact ttattatccg caatattaaa gccaaaaata ttacgcctga tttcagtaaa
     6421 aaggcgggaa tagacaatgc cacggtagca atttatggct gtgataattt cgttattgat
     6481 aatgtcgata tggtcaatag tgccggcatg ttaatcggtt acggcgtgat taaaggtgac
     6541 tacctgtcga ttccgcagaa cttcaagctt aacgatattc gtctggataa tagccagctt
     6601 gcctataagc tgcgcggcat acaaatttca tccggtaatg cgacctcatt tgtggccatc
     6661 accaacgtcg acatgcagcg cgcgacgctt gaactgcata acaaacctca gcaccttttc
     6721 ctacgcaata tcaatgtgat gcaggaagca gcaatcggac ccgcgctgaa gctgaacttc
     6781 gacctgcgta aggatgtgcg gggtaagttt atggcgaaag acgagacgtt gctgtcgctg
     6841 gcaaacatca aagcagtgaa tgagaaaggg cagagctcgg tggatattga ccgggtagat
     6901 cagcatgtgg tgaatacgga acggctcaat tttgcgctgc cgcacagata atcgtcagtg
     6961 ggctttttgc gactattcct ggcacggaat cgcattctat tcagattaca actactgtaa
     7021 tttgtggtgc taaggcgtaa tatcattaca ggattacggc aaatcatact ggcgaaaaca
     7081 ggtttaagag ctataattcg tcaaccattt taaggtggaa gaaataatga ttaatttgaa
     7141 agcagtcatt ccggtagccg gactgggcat gcatatgctg ccagccacaa aagccattcc
     7201 taaagagatg ctaccgatcg tcgacaagcc gatgattcaa tacatcgtcg acgagattgt
     7261 tgctgcaggg atcaaagaaa tcgttctggt tacgcactcg tccaagaatg cggttgaaaa
     7321 ccacttcgac acctcttacg aactcgaagc gctgctggag cagcgtgtta aacgtcaact
     7381 gctggcggaa gtgcagtcca tttgtcctcc tggcgtgacc atcatgaacg tgcgtcaggc
     7441 gcagccgctg ggcctgggcc actccatcct gtgtgctcgc cctgtcgtgg gcgacaaccc
     7501 gtttatcgtg gtcctgccgg atatcatcat cgataccgct tctgcggatc cgctgcgcta
     7561 taacctggcg gcgatggtgg cgcgtttcaa cgaaacaggc cgcagccagg tgctggcgaa
     7621 acgcatgaaa ggcgatctgt ccgagtactc tattatccag actaaagaag cgctggagac
     7681 agaagggcag gtgagccgca tcgttgagtt catcgaaaaa ccggatcagc cgcagacgct
     7741 ggattctgac ctgatggcgg ttggtcgtta tgtcctgaac gcggatatct gggccgagct
     7801 ggaaaaaacc gagccaggcg cctgggatcg tatccagtta accgatgcga tcgccgagct
     7861 ggcgaaaaag cagtctgttg acgcgatgct gatgaccggt gagagctacg actgcggtaa
     7921 gaagctgggc tacatgcagg cgtttgtgaa ctatgggctg cggaacctga aggaaggggc
     7981 gaagttcaga agccggattg agaagttgtt agctaacgac tgattttatc ttagttgatt
     8041 tgcacaagcg gcaatcatca tttggggatg ttgaaacata accaaaatgg tagctgccgt
     8101 tttttgttat taattctaat gataacatgg atttatctga tttaaatctg gtcagatttg
     8161 taacgatttg tgcttgtttc tgaggtcgtt taggacgaca attagcagag ttgtaatgca
     8221 gttgtctagt ggcagttagt gtccgatgac agacaacaga aaatgaaaga atcatgcatc
     8281 attcagtgca ctggtagctg ttgagccagg ggcggtagca tatttaattg tgagagaaca
     8341 atgaagagaa ttattacgtt tggtacattt gatgtttttc atgttggtca tgtaaatatc
     8401 cttgagcgag ctgcttcact tggcgactat cttattgtag gtgtaagttc agataaattg
     8461 aatttcaata aaaaaggtcg ttaccccatt tataatcaag aagaccgctg tcgtataata
     8521 aattctctta gagttgtgaa tgatgtattt attgaagaat ctttagagca aaaaaaagag
     8581 ta
//
LOCUS       DAFNBJ010000188         8471 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000188 DAFNBJ010000000
VERSION     DAFNBJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.188"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            141..2519
                     /gene="ppsA"
                     /locus_tag="L2X49_004256"
     CDS             141..2519
                     /gene="ppsA"
                     /locus_tag="L2X49_004256"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBN4799251.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPGLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2576..4222)
                     /gene="fadK"
                     /locus_tag="L2X49_004257"
     CDS             complement(2576..4222)
                     /gene="fadK"
                     /locus_tag="L2X49_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBN4799252.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4279..4572)
                     /locus_tag="L2X49_004258"
     CDS             complement(4279..4572)
                     /locus_tag="L2X49_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBN4799253.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4569..5858)
                     /locus_tag="L2X49_004259"
     CDS             complement(4569..5858)
                     /locus_tag="L2X49_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBN4799254.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNNDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5914..6852)
                     /locus_tag="L2X49_004260"
     CDS             complement(5914..6852)
                     /locus_tag="L2X49_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HBN4799255.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6872..7636)
                     /locus_tag="L2X49_004261"
     CDS             complement(6872..7636)
                     /locus_tag="L2X49_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBN4799256.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLTTDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQPQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            7952..>8471
                     /locus_tag="L2X49_004262"
     CDS             7952..>8471
                     /locus_tag="L2X49_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBN4799257.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQ"
BASE COUNT         2020 a         2186 c         2205 g         2060 t
ORIGIN      
        1 acgctgggat cagtcttaaa aagtaaaaaa atatatttgc ttgaacgatt caccgttttt
       61 ttcatccggt taaatatgca aagataaatg cgcagaaatg tgtttctcaa accgttcatt
      121 tatcacaaaa ggattgttcg atgtccaaca atggctcgtc accgctggtg ctttggtata
      181 accaactcgg catgaatgat gtagacaggg ttgggggcaa aaatgcctcc ctgggtgaaa
      241 tgattactaa tctttccgga atgggcgttt ccgttccgaa tggtttcgcc acaaccgccg
      301 acgcgtttaa ccagtttctg gaccaaagcg gcgtaaacca gcgcatttat gaactgctgg
      361 ataaaacgga tattgacgat gttacccagc ttgcgaaagc gggcgcgcaa atccgccagt
      421 ggattatcga cactcccttc cagcctgagc tggaaaacgc catccgcgaa gcctatgcac
      481 agctttccgc cgatgacgaa aacgcctcgt ttgcggtgcg ttcctccgct actgcagaag
      541 atatgccgga cgcttctttt gccggtcagc aggaaacttt cctcaacgtt cagggttttg
      601 acgccgttct cgtggcagtg aaacatgtat ttgcttctct gtttaacgat cgcgccatct
      661 cttatcgtgt gcaccagggt tacgatcacc gtggcgtggc gctctccgcc ggtgttcagc
      721 ggatggtgcg ctccgacctc gcatcttctg gcgtgatgtt ctccattgat accgaatccg
      781 gctttgacca ggtggtgttt atcacttccg catggggcct tggggaaatg gtcgtacagg
      841 gtgcggttaa cccggatgag ttttacgtgc ataaaccgac actggcggcg aatcgcccgg
      901 ctatcgtgcg ccgcaccatg gggtcgaaaa aaatccgcat ggtttacgcg ccgacccagg
      961 agcacggcaa gcaggttaaa atcgaagacg taccgcagga acagcgtgac atcttctcgc
     1021 tgaccaacga agaagtgcag gaactggcaa aacaggccgt acaaattgag aaacactacg
     1081 gtcgcccaat ggatattgag tgggcgaaag atggccacac cggcaaactg ttcattgtgc
     1141 aggcgcgtcc ggaaaccgtg cgctcacgcg gtcaggtcat ggagcgttat acgctgcatt
     1201 cacagggtaa gattatcgcc gaaggccgtg ctatcggtca tcgcatcggc gcgggtccgg
     1261 tgaaagtcat ccatgacatc agcgaaatga accgcatcga acctggcgac gtgctggtta
     1321 ctgacatgac cgacccggac tgggaaccga tcatgaagaa agcatcagcc atcgtcacca
     1381 accgtggcgg tcgtacctgt cacgcggcga tcatcgctcg tgaactgggc attccggcgg
     1441 tagtgggctg tggtgatgca acagaacgga tgaaagacgg tgagaacgtc actgtttctt
     1501 gtgccgaagg tgataccggt tacgtctatg cggagttgct ggaatttagc gtgaaaagct
     1561 ccagcgtaga aacgatgccg ggcctgccgt tgaaggtgat gatgaacgtc ggtaacccgg
     1621 accgagcttt cgacttcgcc tgtctgccga acgaaggcgt gggacttgcg cgtctggaat
     1681 ttatcatcaa ccgtatgatt ggcgtccacc cacgcgcact gcttgagttt gacgatcagg
     1741 aaccgcagtt gcaaaacgaa atccgcgaga tgatgaaagg ttttgattct ccgcgtgaat
     1801 tttacgttgg tcgtctgact gaagggatcg cgacgctggg tgccgcgttt tatccgaagc
     1861 gcgtcattgt ccgtctctct gattttaaat cgaacgaata tgccaacctg gtcggtggtg
     1921 agcgttacga gccagatgaa gagaacccga tgctcggctt ccgtggcgcg ggccgctatg
     1981 tttccgacag cttccgcgac tgtttcgcgc tggagtgtga agcagtgaaa cgtgtgcgca
     2041 acgacatggg gctgaccaac gttgagatca tgatcccgtt cgtgcgtacc gtagatcagg
     2101 cgaaagcggt ggttgaagaa ctggcgcgtc aggggctgaa acgtggcgag aacgggctga
     2161 aaatcatcat gatgtgtgaa atcccgtcca acgccttgct ggcagagcag ttcctcgaat
     2221 atttcgacgg cttctcaatt ggctcaaacg atatgacgca gctggcgctc ggtctggacc
     2281 gcgactccgg cgtggtgtct gaactgttcg atgagcgcaa cgatgcggtg aaagcactgc
     2341 tgtcgatggc gattcgtgcc gcgaagaaac agggcaaata tgtcgggatt tgcggtcagg
     2401 gtccgtccga ccacgaagac tttgccgcat ggttgatgga agaggggatc gatagcctgt
     2461 ctctgaaccc ggacaccgtg gtgcaaacct ggttaagcct ggctgaactg aagaaataaa
     2521 ataaatcccc ggcggcgttc agtcgccggg gttatgtgat ccccgaagat gatacttatt
     2581 caatctcttc acagacatcc tgcgttaatc gccgcataat atcttttctt aacaaaaact
     2641 tttgtatttt acctgaggca gtgcgcggta gtttttcgat taccacgata tgttcaggat
     2701 atttatattt tgcgacccgt ttacggctaa aaaaagccac cacctcttcc agcgataatg
     2761 aatgatgcgg cgctttcagc acgacataag cgcatgatcg ttcacctaaa cgttcatcgg
     2821 gcattgcaac cacacaggca tcgtgaattt taggatgctg caataaaata tcttccactt
     2881 cacggctgct aatgttttcg ccgccgcgga caataatatc ttttttgcgt ccggtaattt
     2941 ttatatagcc agcctcatcc atgcggcaga gatcgccgct gtaataccag ccttcttcgt
     3001 ccagcgcacg ggcggttaat tcaggttcat caaaataccc cataaacaca ttggggccac
     3061 gcgaggcttc ttcaccttcg caacctggcg gtaaggtctt gcgtgcgtca tcgaccactt
     3121 taatctctac acctgcggca gcgtaaccat cggtgtgcat aaagcgcgac aaaggatcat
     3181 cgagattcac caccgcatgc ggcgaacttt ctgtggaacc ataaacactt aataatttaa
     3241 tgccgcgctg ctggcattca cgtgccactt ttttggggat tgtggtaccg ccgcaaagaa
     3301 agaaacgcag cgctgaaagg tccgcgggtt gtttctctag tacattcaaa agatcataga
     3361 caaacggcgt tgcgcctagc atacaggtgc aacgctgctg ctcaagcagc gcgagacacg
     3421 catcaggagt gaaaatatct aacaacacgc tgcgagcgcc aattaagaat ggtgccgtta
     3481 cgccatgcag aaagcccgtt gcgtgaccaa gtggcgcagg catcataaag acatcctgcc
     3541 aggtcagatt cagtcgcgcg caataagccc gctcactggc gagaatattg ttatgcgtta
     3601 gcatcacgcc ctttggcaga ccctcggttc cggaggtaaa cagcaccgca gctaattcat
     3661 cgccgtgggt cgttatcgcc gtggtcagtg aggtattgtc ggcgataatc tgacttaatg
     3721 agagggaaga ggtggcggga gccagtttgt ccacaccgac aatttgttgt agttgtggaa
     3781 gctgattttg cagcggcagg attaaatcta ccggacgcgt ttgtttaaac aacgtcggtg
     3841 caaagaacat ttttgcctga cacttattga gcacccacac cagttctgct tcccgccagg
     3901 aaggcaacag cggcacggaa accgcaccgg ttttcaggca ggcaagatag ataacggtaa
     3961 attcacacca gcctggcagt tgaaatgcga tgcgatcgcc tgattcaata cccttcgcta
     4021 acatccagtt tgccagacag ctcgcggcgt gatcgagcgc gctataggtg tacgatgctc
     4081 catgattatc gaccacggca attttgtctg gcatcgcacg agcggtctgc tgccagtaat
     4141 cggccagcga agcatcgccc catagccctt gctgacgata cgccgcacga cgttgttcgt
     4201 tgaacgttaa tgtcactttc atgttggact ctcgaaacag aacatcaggc ccgagatgcg
     4261 ggcctgtggg atgcatgatt agccgtagcg aaaatcaata ccgaaggtgc ctgcgggata
     4321 ttgccactgt tcgagaatag tgttaccgca cagcacccga caggtgccgc actccagaca
     4381 accggcagaa tcaaaatgaa tgttgccggc gtcatcctgc ttataaagtc cggcagggca
     4441 ggctttcatt aatttacgga attcattgat atcgggattt gccgccagaa tgatatgcgg
     4501 gtggccctca tcaacatgga atttattgac gcctaatttg atgtcgacgt taaccgtagc
     4561 gttctggctc atagcgcggt tgctccctta atgccatctt tcagcaagtt gatcagccca
     4621 attttcttcg catgtcccat gatcattttg cgtaccggct ggtttggttt gccgtcaatg
     4681 gtgaacatct cgttcatgat gtcggcgacc attcgtgggt attggctaaa caggcgcggg
     4741 ttttccatca gcgccgggat cttgcggaaa tgctgcatat cgcgcatgac gcagctttgt
     4801 tccagctcac gtttgtattg cgccagactg ctggcggaga aatcctcgcg ttctttggcg
     4861 gcgatcaccg ttgtggcggc agcctgagcc gatgcaatgg ctaaatccat gccacgaact
     4921 gtaaaaccca aattcaggca gaagcctgcg gcgtcaccaa cgatcatcac gccatcgtta
     4981 accagctgcg gcaccattgc cagaccgcct tctggcacca tatgcgcgga atattcaagc
     5041 agtttgccgc cgctaattag tgggcgaatg gcggggtgtt gtttaaaatc ttccagcatt
     5101 tgcggcacgc ttttttgcgc atgggcgata tcacccaatc cacaaaccag ccccaacgat
     5161 acggaatcgt tgttggtata gaggaatccg ccacccatca ggccgtcaga aggggaaccg
     5221 gcaaacagcc aggcggcacc ttcctcgccc gtaatattaa agcgatcgtt gatctgttct
     5281 ggcgtgaggc caataacctc tttaacacca acagcgtaat gatgcggatc ggaagcggga
     5341 accattccca gcgagcggcc aagcatcgag ttaacgccat cagccagaat caccacattc
     5401 gcttcgagaa tatcatcccc agcctgcacg ccagttacct tgtttccttc acgaaccagc
     5461 gcatcgacgc gaacgcctgg gataaactgc gcgcccgcct gctcggcttg ttccatcaac
     5521 cacgggtcca gacgattacg caatacggta taagatgcgt gttgcggaac atctggctgc
     5581 tcgcggtgaa aatcgagggt aacggcgctt tcttcggtta agaaggagat tttctcgcgt
     5641 gtgaccttgc gttctaccgg cgctgatgct gcaaagcctg gaatgattgc ttcaagtgtg
     5701 tgggcataaa gacgcccgcc ggtcatgttt ttacatccgg cactgtcgcc gcgttctatc
     5761 accaggacat ccagcccggc tcgtgccatg accagtgcgg caacgctacc agcaacgccc
     5821 gcaccgacca caatggcatc aaatttgtca tccgacatgc gttatgctcc cttcccgctg
     5881 cgtttacagc gggagatgtg gttgaaaggg gaattagcgg ctcaactggc taatcagggc
     5941 agggacgact ttgtagatat cgcccaccag accgtagtcg gcatagttga agattggcgc
     6001 atttttatct ttattgatgg cgacaatcac ttttgcgccg ttgccgccaa ccatatgttg
     6061 gatctgcccg gagatcccca gcgtcaggta gagatcagat ttcagcaaca cgccggagac
     6121 accgatataa cgttcacgct ccatccagtt ctcgccttcg gcaattggac gtgaacagcc
     6181 gacttcagca ttcagcaccg ccgccagttc gtggaccatt ttcaggtcat cctgcgccgc
     6241 aagaccacga ccgacgccaa ccacacgttt tgctttgctc aggtccacgc tgcttgcggc
     6301 tttcgcacgg cgttcgcgac agagaatttc atgacgcgga gcaacataag gtaccgtttc
     6361 tgtcgggcac tgatgagagg tatcactggt gcacggttcc tgaacaccgg gtgcaagggt
     6421 aatgatcgcc agcgggctgt tgatcttttc ctgagcgaac gctaacccgc catacatccg
     6481 gtgttcggcg caaatgtgac catcgacaat atccaccgcc gtggcatcgt tcaccagcgc
     6541 cgcattcagt tgcacactta accgtgctgc cagagcttta ccacgtttcg tcgcggccaa
     6601 cagcaacata gcggggtgtt tatctttcag cagggcagca atgctttcgg cgtaattttc
     6661 agtgcgttgc agcgcgtcgt tttgctcaag aacataaata cattttggac cataaggcat
     6721 aactgcctgc gcctggtcgg tattttgtac aatggcatac acctgttggc cccattgctg
     6781 agcgccgcca aacagttcag cataacgttc aggattatcg ctaaagaccc agacgctgtt
     6841 taattgactc atcgtttctt ctccgtaggc ttcagttcag ggctttcttc agatgttccg
     6901 ccagctcggc aatggcctcc ggcgaatcgt tatcgataat gatgtgctta cgttctgttt
     6961 gcggcggtac gcgaatgccg accagttcag caagtggcgc gctctggctc cagtcaatat
     7021 cacttgcctg ccactgattt accggttttt tacccgcgcc gagaatggct ttcatcgaag
     7081 gaatgcgtgg cacgttaata tcggaggtga cgcagagcac ggctggaaca gagagttcaa
     7141 taacttcaac atcatcttca agcgtgcgtt caatcaccag tgtattgccc tgaggctgaa
     7201 tagcactcac tgcattaatc accggaagtt gcagaatttc tccgaccagc aagccaacct
     7261 gctgggcata aaggtcgccg gaaccttcac caaagatcag taaatcgaag ccgatctttt
     7321 caattgctgc cgccagcgct tttgcggtat cgagaggcag tgcatgttca agttgcgcat
     7381 cctgcaccaa atacaggctg tgcggcccgc gggatagcac gtctttgcgc actttcgagt
     7441 tctgcaacaa tgagccgcca acggtcagcg cggctatctc atcgtcatct gttgtgagct
     7501 ggcttgcagc ttcaatggca ttgagatcga actggctgat tttggcgtcg gcattgtcga
     7561 aattcagggt gtattctgga gtgacaacaa tgtcctgttc ttcaggcacc agcttaaagc
     7621 aggttattat tttcatgatt tctcctgtga gtcgcatacg gaaccatctc cgatggaagg
     7681 taaaaatggc ctgaaaatag tgtgcgatag cggtgcgggg ttttcgacgg agcaagctgt
     7741 tttccttgcc aacaatcttg caaaaaaaat gcgatcgcta aaacaaaagt gcaatttgcg
     7801 gcaatatagt tattccatga ccgtacaatc ttgcattcag tgtaaataat cttgtttagc
     7861 tctcgctaaa cgcggttgta taaccgcaat acacgccgta ttttatccgg catatattgt
     7921 ttttgcttgc tcatggaata gggaggttgt catgtatcaa cgctgttttg ataacgccag
     7981 tgaaacgctg tttgtcgccg gtaaaacgcc acggctttca cgttttgcat ttagcgatga
     8041 tccaaaatgg gagtccggac atcacgttca tgacaatgaa accgagttga tttacgtcaa
     8101 gaaaggggtt gcaaggttta ccatcgattc ttcgttatat gtcgcacatg cggatgacat
     8161 tgtggtgata gaacgcggca ggctgcatgc ggtggcctct gacgttaacg atccggcaac
     8221 gacgtgtacc tgtgcgctgt acggctttca gtttcagggg gctgaggaaa atcagctact
     8281 gcaaccgcat tcttgtccgg taattgccgc ggggcaggga aaagaagtca taaaaacctt
     8341 atttaatgag ctaagtgtga ttttgccgca aagtaaaaat agccaaacat cttcgttatg
     8401 ggatgcattt gcctatacgt tagccattct ttactacgaa aattttaaaa atgcttatcg
     8461 ttcggagcag g
//
LOCUS       DAFNBJ010000189         8463 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000189 DAFNBJ010000000
VERSION     DAFNBJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.189"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            4..714
                     /locus_tag="L2X49_004263"
     CDS             4..714
                     /locus_tag="L2X49_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBN4799258.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            756..3407
                     /locus_tag="L2X49_004264"
     CDS             756..3407
                     /locus_tag="L2X49_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBN4799259.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSATNGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGPRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            3421..3951
                     /locus_tag="L2X49_004265"
     CDS             3421..3951
                     /locus_tag="L2X49_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBN4799260.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            3964..4467
                     /locus_tag="L2X49_004266"
     CDS             3964..4467
                     /locus_tag="L2X49_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBN4799261.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4527..5441
                     /locus_tag="L2X49_004267"
     CDS             4527..5441
                     /locus_tag="L2X49_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4799262.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            5775..8054
                     /gene="ydeP"
                     /locus_tag="L2X49_004268"
     CDS             5775..8054
                     /gene="ydeP"
                     /locus_tag="L2X49_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBN4799263.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKNGAQAVTVEDSMSMIHASRGMLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            8302..>8463
                     /gene="safA"
                     /locus_tag="L2X49_004269"
     CDS             8302..>8463
                     /gene="safA"
                     /locus_tag="L2X49_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBN4799264.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARD"
BASE COUNT         2385 a         1865 c         1939 g         2274 t
ORIGIN      
        1 cagatgcaaa ctacgcgtac cccctactct atttccttta tggcgacagt gttactttta
       61 ttgctctttg cttgccatag caccgttgcc aacgcagctg tcgcactggg tgcgacgcgg
      121 gtaatttatc cggcaaacca gaaacaggtt ttattgcctg tcacgaataa tgatcctgcc
      181 agtgtctatc tcattcagtc atggatagaa aatgcaggtg accaaaaaga cacccagttt
      241 gtcattaccc caccgctttt ttccatgcaa ggtaaaaaag agaatacgct gcggataatc
      301 aacgctacta atcatcagtt accaggcgac cgtgaaagcc tgttctgggt gaatgttaaa
      361 gctattcctg ctatggaaaa agaccagaag aatgagaata cattacagct ggcaattatt
      421 agccgcataa aaatgtttta tcgccccacg aacctggcaa tggcacctga agaagcgcct
      481 gcaatgcttc ggtttcgtcg cagcggtagc aaactcacgc ttattaatcc aacgccctat
      541 ttcattaccg taacaaatat gaaagcagga aatagcaatc tcccaaacac catggtgccg
      601 ccaaaaggtg aagtatcggt ggatatcacg catgctgcaa ccggggatat cagttttcag
      661 acgatcaatg attacggtgc attaactccg cgtattaagg caaccatgca gtaagccagt
      721 gacctaatta ataataaact caatcggaat taataatgac agccattcgt gccgcattta
      781 aggcatatcg tatgcatcag gtgttaataa tgcctcgctt tgcacggctt accattgccc
      841 ttggcttagc aactgcggta tttcccgttg acgcagagta ttattttaat ccacggtttt
      901 taagcaatga tcttgctgaa tctgtcgatt tatccgcctt tactaaggga cgcgaggcac
      961 cacctggtac atatcgggtc gacatttatt tgaacgatga atttatgact agtcgggata
     1021 ttacttttat tgcagatgat aataatgcag aactcatccc atgcctgagt acagatcttc
     1081 ttgtcagtct tggtataaaa aaatcagcgt tattagacaa taaagaacat tcggcagaaa
     1141 aacatgtgcc ggacaacagc gcatgcacac cacttcagga tcgtttggcc gacgcatcaa
     1201 ctgagtttga tgttggtcag caacatttgt cactctccgt tccgcaaatt tatgttggca
     1261 gaatggctcg cggctatgtt tctcctgatc tgtgggaaga aggaataaat gctgggcttc
     1321 taaactatag ttttaatggt aattctatta ataatcgtag taaccataat gcaggaaaat
     1381 caaactatgc atatttgaat ttacagagtg gcatcaacat tggtagttgg cgactacgcg
     1441 ataactcaac gtggagttat aacagtggga gtagcaattc atctgacagc aataaatggc
     1501 agcatatcaa tacgtcggct gaacgtgaca ttattccctt acgctcacgg ttaacggtag
     1561 gtgatagtta taccgatggc gatatttttg atagtgtgaa ctttcgtggc ctcaaaataa
     1621 attcaacaga agcgatgttg cccgatagcc aacatggttt cgctcctgtg attcatggta
     1681 ttgcccgtgg aaccgcacaa gtgagtgtaa aacagaacgg atacgatgtt tatcagacta
     1741 ctgttccacc cggccctttt actattgatg atatcaactc tgcgaccaat ggtggtgatc
     1801 tgcaagtaac cataaaagag gcagacggca gtattcagac attatatgtt ccttattcgt
     1861 ctgttccggt tctccaacgt gctggatata cgcgttatgc gcttgccatg ggggaatatc
     1921 gtagtggaaa taacctgcaa agctctccca agttcataca aggtagcttg atgcatggtc
     1981 tggaaggaaa ctggacacct tatggcggaa tgcaaattgc agaagattat caggccttca
     2041 accttggtat tgggaaagat ttaggacttt ttggtgcctt ttctttcgat atcacgcagg
     2101 ccaatacgac acttgcagat ggcccccgtc acagtgggca atcggttaaa tccgtctaca
     2161 gcaaatcctt ctaccagaca ggaaccaata tccaggtcgc aggatatcgc tattctacgc
     2221 aaggttttta taacttatcc gacagtgcct acagtcgaat gagtggttac accgtcaagc
     2281 ctcctaccgg agacaccaat gagcagacac aatttattga ttattttaat ctgttctaca
     2341 gtaagcgtgg tcaggaacaa ataagcatct ctcagcagct tggaaattac ggtacgacat
     2401 ttttcagtgc cagtcgccaa agttactgga acacgtcacg cagcgaccag caaatatcat
     2461 ttggattaaa tgtgccgttt ggtgatatta cgacttcgct gaattacagc tattccaata
     2521 atatatggca aaacgatcgg gatcatttac tcgcttttac gcttaatgtt cccttcagtc
     2581 attggatgcg tacagacagt cagtcggcat ttcgtaattc aaacgccagt tacagtatgt
     2641 caaacgattt gaaaggcggc atgaccaatc tatcgggggt ttatggcact ctgctgccgg
     2701 ataataacct gaattatagc gttcaggtcg gtaacaccca cggaggtaat acatcgtctg
     2761 gcaccagtgg ttacagttct cttaattatc gtggagctta cggtaatacg aatgtcggtt
     2821 acagtcggaa tggtgacagc agccagattt attacggaat gagtggtggg attattgctc
     2881 atgctgatgg catcaccttt ggacagccgc tgggcgacac aatggttctg gttaaggctc
     2941 ctggcgctga taatgtcaaa atagagaacc agaccggaat tcataccgac tggcgtggct
     3001 atgcaatatt accatttgcg acagaatata gagaaaatcg tgtcgctctt aacgcgaatt
     3061 cccttgcaga taatgttgaa ctggatgaaa ccgtagtcac tgtcatccca actcacggtg
     3121 ctattgccag agcaacattt aatgcacaaa tcggcgggaa agtattaatg acgttgaagt
     3181 acggtaataa aagcgttcca ttcggtgcaa ttgtcactca cggagagaat aaaaatggca
     3241 gcattgtcgc ggaaaatggt caggtttatc tgactggact tccacagtca gggaaattac
     3301 aggtttcatg gggcaatgat aaaaactcaa actgtattgt cgattacaag cttcctgaag
     3361 tctctcctgg aaccttgctg aaccagcaga cagcaatctg tcgctaagga acaatagatt
     3421 atgaaataca ataacgttat tttcctcggt ttatgtctgg ggttaaccac ctattctgct
     3481 ttatccgcag atagcgttat taaaattagc gggcgcgtcc tcgattatgg ctgcacagtc
     3541 tcatcggatt cgcttaattt taccgtagat ctccaaaaaa acagtgccag acaatttcca
     3601 acgaccggta gcacaagtcc agccgtccct tttcagatta cgttaagtga atgcagcaaa
     3661 gggacaacgg gggttcgggt tgcatttaac ggtattgagg atgcagaaaa taatactctg
     3721 ttgaaactgg atgagggaag caatacggcc tccggtttag gtatagaaat actggacgga
     3781 aatatgcgtc cggtgaaact gaatgacctt catgccggga tgcagtggat cccactggta
     3841 ccagaacaga acaatatttt gccttactcc gctcgtctga agtcaactca gaagtccgtc
     3901 aatccgggac tggtgagggc ttcggcaacc tttacccttg aatttcaata atcagagtca
     3961 gatatgaaaa gacttcacaa gaggttcctg ttagctacgt tttgcgcgtt attcacagca
     4021 actctccagg ccgccgatgt cactatcact gttaatggtc gggtagtcgc taaaccctgc
     4081 actattcaaa ccaaagaagc taacgttaat ctcggggatc tttatacgcg caatctgcaa
     4141 caacctggtt ctgcatctgg ctggcacaat attactttgt cattaaccga ttgtccggtt
     4201 gaaacaagtg cagtgacggc aatcgtgaca ggttcaactg acaatacggg ttattacaaa
     4261 aatgaaggta ctgccgaaaa tattcagata gagctgaggg atgaccagga tgcgacgtta
     4321 aaaaatggcg atagcaaaac ggttattgtt gatgagatca ctcgtaatgc acagtttcca
     4381 cttaaggcaa gagctatcac ggtgaatgga aacgcaagcc agggaacgat cgaggcgcta
     4441 atcaatgtga tctacacctg gcaataacgt cgattcgatt ttcttagcct tctttttaaa
     4501 tcaatttttt aaccttagga tatatcatgg gtaaaacaat cagtataaag gtcctgttcg
     4561 gtatttatct tctattaatg gcaggtaaag tttttgcctt cagttgtaat gtcgatggcg
     4621 ggtcaagtat tggagctggt acaacctctg tttatgttaa tctcgaccct gtaatacagc
     4681 cgggccagaa tctggttgta gacttgtctc agcatataag ttgctggaat gattacggcg
     4741 gctggtacga cactgatcat ataaacctgg tacaaggctc agctttcgca ggctctctac
     4801 agtcatataa aggatcgctg tactggaata atgtgaccta ccccttcccg ttgacgacaa
     4861 ataccaacgt attggatatt ggtgataaga caccgatgcc attaccgtta aaactctata
     4921 ttaccccagt cggggcggcc ggtggagttg tcataaaagc cggggaagtt attgcccgta
     4981 ttcatatgta taaaattgca acattaggaa gcggaaatcc gcgtaatttt acgtggaata
     5041 ttatttcaaa taacagtgtt gttatgccaa caggcggctg cactgttgat agtcgtaatg
     5101 tcaccgtgga tctcccggac tttcctggaa gcgcagaaat accccttggc gtatattgct
     5161 caagcgaaca aaagctctca ttttatcttt cgggtgccac cactgacagt gcccgacaag
     5221 tgtttgctaa cacagcacct gatgcaacaa aagcatcggg agtgggtgtc tctctgatgc
     5281 gtaatggaaa aatactggct acgggagaaa atgtctccct gggaacagta aataaaagca
     5341 aagtacctct gggtttaagc gcaacctatg gacaaacagg aaacaaggtt tcagccggaa
     5401 ccgtccagtc agtcattggt gtcactttta tctatgaata attgtgattt tgatcttagt
     5461 ctatgcgctc cattcacgaa aattgcgcgg ataattattt tgtaaaggct attatcctac
     5521 acctgtaaga aaatccgcgc acaattgaag ccagagtctt attttttata ttgaaaaata
     5581 tgttaattta aacactatga gagatgatgc ttaacttatc gtatttaatc tattgtttaa
     5641 cgataataga ttaaaaatag aaagagagta gtcccctact cttatatatc catgttggcg
     5701 ataatccctt tgtatatact gtgcatcatc gctattacaa atcctaataa ttcatttcca
     5761 cacaggataa gtagatgaag aaaaaaattg aatcctacca gggtgctgcc ggtggttggg
     5821 gtgctgttaa atccgtagcg aatgcagtac gtaagcagat ggatatacgc caggatgtta
     5881 ttgccatgtt tgacatgaat aagccagagg gctttgactg tccgggttgt gcatggccag
     5941 atcctaagca cagtgcgtca ttcgacattt gtgaaaacgg cgcaaaagca atcgcctggg
     6001 aagtcacgga taagcaggta aatgcctctt tctttgctga gaatacggtt caatcattac
     6061 ttacctgggg agatcacgag cttgaggctg cgggacgtct cactcagcct ttgaaatatg
     6121 atgccgtcag cgactgttac aagccattaa gctggcaaca agctttcgac gaaattggcg
     6181 cacgccttca aagctatagt gatcccaatc aggttgaatt ctatacttcg ggccgcactt
     6241 ccaatgaagc tgcctttctt tatcagcttt ttgcccgtga atacgggagc aataactttc
     6301 ccgactgctc caacatgtgc catgaaccga caagcgtggg tttggcagcg agtatcggtg
     6361 taggtaaagg gaccgtgttg ctggaagact ttgagaagtg cgatttagtc atttgcattg
     6421 ggcataaccc tggtacaaac caccctcgca tgctgacttc gttgcgcgct ttagtgaaac
     6481 ggggagcgaa aatgatcgcc atcaatcctc tacaggaacg tggcctggag cgatttaccg
     6541 caccgcaaaa cccgtttgaa atgctgacga actctgagac tcagttggcc agtgcctact
     6601 ataacgtgcg cattggtggc gatatggcgt tgctcaaggg gatgatgcgc ctgttaattg
     6661 agcgcgatga tgctgcaagc gccgcaggtc ggccttcatt gcttgatgac gagtttattc
     6721 aaacgcatac cgtcggcttt gacgagctac gccgtgacgt tctcaattcc gagtggaaag
     6781 atatcgaacg tatttctgga ctaagtcaga cacaaatcgc cgaactggct gacgcatatg
     6841 ccgctgccga acgcaccatt atctgttacg gaatggggat cactcagcac gaacatggta
     6901 cccagaacgt acagcaactg gtcaatctgc tgttgatgaa aggtaacatt ggcaagcctg
     6961 gtgcgggtat ctgcccacta cgtggacact ctaatgtaca gggcgaccga accgtcggta
     7021 tcaccgagaa accgtctgca gagtttctgg ctcgtctggg tgagcgctat ggcttcaccc
     7081 cacctcatgc acctggacat gctgcaattg ccagcatgca agcaatatgt acggggcagg
     7141 ctcgagcatt gatctgcatg gggggcaatt ttgcgctggc aatgccagat cgggaagcga
     7201 gcgctgtacc gttaacgcaa ttagatttgg cggtacacgt agccactaag cttaaccgct
     7261 ctcatctgtt gaccgcacgg catagctata ttctgccggt tctgggacgt agcgagattg
     7321 acatgcaaaa aaacggtgcg caggcggtaa ccgttgagga ttcaatgtcg atgattcatg
     7381 cctcgcgtgg catgttaaaa cccgccggtg taatgctgaa atcagagtgt gcagtggtcg
     7441 cgggaatcgc gcaggcagca ctaccccaga gcgtggtagc ctgggagtat ctggtggaag
     7501 attatgatcg cattcgcaat gacattgaag ctgtgctgcc agagttcgcc gactataacc
     7561 aacgcatccg tcatcccggt ggttttcacc tgataaatgc agctgctgaa aggcgctgga
     7621 tgacgccgtc aggtaaggct aatttcatta ccagcaaagg gctgttagaa gatccctctt
     7681 cagcgtttaa cagtaagctg gtcatggcga cagtacgcag tcacgatcaa tacaacacga
     7741 cgatttatgg tatggatgat cgctatcgag gggtattcgg tcaacgagat gtggtcttta
     7801 tgagtgctaa acaagctaaa atttgccgtg taaaaaacgg cgaaagagtt aatcttattg
     7861 cgcttacgcc agacggtaag cgcagctcac gccgcatgga tagattaaaa gtggtcattt
     7921 accctatggc tgaccgctca ctggtgacct attttccaga atcgaatcac atgctaactc
     7981 ttgataacca cgatccatta agtggcattc ctggttataa aagtattccg gttgaattag
     8041 aaccatcaaa ttaattcgcc tcctcatttc ttctgctgtc atccgcacag cagaagaatt
     8101 tctcattgac tattatcgcg caatttgctc acattgatta aattaaacca cacactataa
     8161 gatataaacc tctgcctaca gctgtaagaa actccgctca gtactgaagt gccagtccta
     8221 tttcctcttt tctctagcct gttatattaa gcatactgat taacgatttt taacgttatc
     8281 cgctaaataa acatatttga aatgcatgcg actacagtga aaaacaaaat cacgcaaaga
     8341 gacaactata aagaaatcat gtctgtaatt gtggttgtct tattactgac ccttacgttg
     8401 atagccattt tttcggcaat tgatcagctg ggtatttcag aaatgggtcg cattgcaaga
     8461 gat
//
LOCUS       DAFNBJ010000190         8428 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000190 DAFNBJ010000000
VERSION     DAFNBJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.190"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            287..1264
                     /gene="csiD"
                     /locus_tag="L2X49_004270"
     CDS             287..1264
                     /gene="csiD"
                     /locus_tag="L2X49_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBN4799265.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1284..2552
                     /gene="lhgO"
                     /locus_tag="L2X49_004271"
     CDS             1284..2552
                     /gene="lhgO"
                     /locus_tag="L2X49_004271"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBN4799266.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAMELREREPNITGLGGIFVPSSGIVSYRNVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2575..4023
                     /gene="gabD"
                     /locus_tag="L2X49_004272"
     CDS             2575..4023
                     /gene="gabD"
                     /locus_tag="L2X49_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBN4799267.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4037..5317
                     /gene="gabT"
                     /locus_tag="L2X49_004273"
     CDS             4037..5317
                     /gene="gabT"
                     /locus_tag="L2X49_004273"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBN4799268.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5555..6955
                     /gene="gabP"
                     /locus_tag="L2X49_004274"
     CDS             5555..6955
                     /gene="gabP"
                     /locus_tag="L2X49_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBN4799269.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6976..7638
                     /gene="csiR"
                     /locus_tag="L2X49_004275"
     CDS             6976..7638
                     /gene="csiR"
                     /locus_tag="L2X49_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBN4799270.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7639..8088)
                     /gene="kbp"
                     /locus_tag="L2X49_004276"
     CDS             complement(7639..8088)
                     /gene="kbp"
                     /locus_tag="L2X49_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBN4799271.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8172..8330)
                     /locus_tag="L2X49_004277"
     CDS             complement(8172..8330)
                     /locus_tag="L2X49_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBN4799272.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1886 a         2205 c         2357 g         1980 t
ORIGIN      
        1 ttactaacgg tcattttgca gtgaagccat ttactgtttt ttatcgacca gataatctgt
       61 tctctaatgt taactctccc taacctgttg ctttagttat tcatttcctg tctcactttg
      121 ccttaatacc ctacgttaaa tgttactaat ttattgcttt tgatcacaat aagaaaacaa
      181 tatgtcgctt ttgtgcgcat ttttcagaaa tgtagatatt tttagattat ggctacgaaa
      241 tgagcatcgc catgtcaccc tacatctcat aagaggatcg cttctgatga atgcactgac
      301 cgccgtacaa aataacgctg tcgattcagg ccaggactat agcggattca ctctcatccc
      361 gtcggcgcaa tccccgcgtc tgctggaact caccttcacc gaacagacga ccaaacagtt
      421 tctcgaacag gttgccgaat ggcccgtgca ggcgctggag tacaaatcgt ttctgcgttt
      481 tcgggtaggc aaaattcttg acgatctgtg tgcgaatcag ctgcaaccat tgctgttgaa
      541 gaccctgcta aaccgcgctg aaggtgcgct gttgatcaat gcggtgggta tcgatgatgt
      601 ggcgcaggca gatgagatgg tgaagctggc aacggcggta gcgcatctga ttggtcgctc
      661 aaatttcgat gcgatgagcg gtcagtatta cgcgcgtttc gtggtgaaaa atgtcgataa
      721 ctcagacagc tatctgcgtc agccgcaccg cgtgatggaa ctgcacaacg acggcacgta
      781 tgtcgaagag atcaccgatt acgtgctgat gatgaaaatc gatgagcaaa acatgcaggg
      841 tggaaattcg ttgctgctgc atctcgatga ctgggaacat ctggaccact atttccgcca
      901 tccactggcg cgtcgcccga tgcgctttgc cgcgccgccg agcaaaaacg tcagcaaaga
      961 tgttttccat ccagtgttcg acgttgatca acagggtcgc ccggtgatgc gctatatcga
     1021 ccagttcgtc cagccaaaag attttgaaga aggcgtgtgg ttgagcgagc tttccgatgc
     1081 cattgaaacc agcaaaggca ttctttctgt acccgttccc gttggcaaat tcctgttgat
     1141 aaacaacctg ttctggctgc acggtcgcga ccgctttact ccgcacccgg atctgcgccg
     1201 tgaactgatg cgtcagcgtg gctatttcgc ttacgccact caccactacc agacgcatca
     1261 gtaagcgcaa aggaattgag cggatgtatg attttgtgat tattggcggc ggcatcatcg
     1321 gcatgtcgac cgccatgcaa ctgattgatg tttatccgga tgcccgcatt gcgttgctgg
     1381 aaaaagagtc cggcccggcc tgtcaccaga cgggccacaa cagcggcgtg atccatgccg
     1441 gggtttatta cacgcccggt agcctgaagg cgcagttttg cctggcggga aaccgcgcca
     1501 ccaaagcctt ttgcgatcaa aacggcattc gctatgacaa ctgcggcaag atgctggtcg
     1561 ccacctctga actcgaaatg gaacggatgc gcgcgttgtg ggaacgcacg gcggcaaacg
     1621 gtatcgagcg cgagtggtta aacgccatgg aactgcgcga gcgcgaaccg aatatcaccg
     1681 ggcttggcgg catttttgtg ccatccagcg gcattgtcag ctaccgcaac gtgacggcgg
     1741 caatggcaaa aatcttccag gccagaggcg gtgagattat ctataacgcc gaagtcagcg
     1801 gcctcagtga gcataaaaac ggcgtggtga tacgtacccg ccagggcggc gaatatgaag
     1861 catcgacgct gattagctgt tccgggctga tggctgaccg gctggtgaaa atgctcggcc
     1921 tcgaaccggg ctttattatc tgcccgttcc gcggcgagta tttccgcctt gcgccggagc
     1981 ataaccagat tgttaaccat ctgatttacc ctattcccga cccggcaatg ccgtttttgg
     2041 gcgtgcatct cacccgaatg atcgatggca gcgtgaccgt cgggccaaac gcggtgctgg
     2101 cttttaaacg cgaaggctac cgcaagcgcg acttctcgtt tagcgacacg ctggaaattt
     2161 taggctcgtc ggggattcgc cgggtgctgc aaaaccatct acgctcagga ctgggcgaga
     2221 tgaaaaactc gctgtgcaaa agcggctatc tgcggctggt gcaaaagtat tgtccccggc
     2281 tttcgttaag cgatctccag ccctggcccg ccggtgtgcg ggcgcaggcg gtatcgccgg
     2341 atggcaagtt gattgacgat tttctgtttg tcaccacccc gcgcacgatc cacacctgca
     2401 atgcgccctc cccggcagcg acatcagcaa ttcctattgg tgcgcatatt gtcagcaagg
     2461 tacaaacgct gttggcaagc cagagtaacc ccggacgcac gctgcgagcg gcacgtagtg
     2521 tggatgcctt acacgccgca tttaatcaat aacctttgaa aacaggacgt aacaatgaaa
     2581 cttaacgaca gtaacttatt ccgccagcag gcgttgatta acggggaatg gctggacgcc
     2641 aacaatggtg aagtcatcga cgtcaccaat ccggcgaacg gcgacaagct gggtagcgtg
     2701 ccgaaaatgg gcgctgatga aacccgcgcc gctatcgacg ccgccaaccg cgccctgccc
     2761 gcctggcgtg cgctcaccgc caaagaacgc gccaacattc tgcgcaactg gttcaatttg
     2821 atgatggagc atcaggacga tttagcgcgc ctgatgaccc tagaacaggg caaaccgctg
     2881 gctgaagcga aaggcgaaat tagctacgcc gcctccttta ttgagtggtt tgctgaagaa
     2941 ggcaaacgca tttatggcga caccattccc ggtcatcagg ccgataagcg cctgattgtt
     3001 atcaagcagc cgattggcgt taccgcagct atcacgccgt ggaacttccc ggcggcgatg
     3061 attacccgca aagccggtcc ggcgctggcg gcaggctgca ccatggtgct gaaacccgcc
     3121 agtcagacgc cgttctctgc gctggcgctg gcggagctgg cgattcgcgc gggcattccg
     3181 gctggggtat ttaacgtggt caccggttcg gcgggcgcag tcggtaacga actgaccagc
     3241 aacccgctgg tgcgcaaact gtcgtttacc ggttcgaccg aaattggccg ccagttaatg
     3301 gaacagtgcg cgaaagacat caaaaaagtg tcgctggagc tgggcggcaa cgcgccgttt
     3361 atcgtctttg acgatgccga tctcgataaa gccgtggaag gtgcgctgtc ctcgaaattc
     3421 cgcaacgccg ggcaaacctg cgtctgcgcc aaccgtttat acgtgcagga cggcgtgtat
     3481 gaccgctttg ccgaaaaatt acagcaggcg gtgagcaaac tgcacatcgg cgacgggctg
     3541 gagaatggcg tcaccatcgg gccgctgatc gatgaaaaag cggtagcaaa agtggaagag
     3601 catattgccg atgcgctgga gaaaggcgcg cgcgtggttt gcggaggtaa agcgcacgaa
     3661 cgcggcggca acttcttcca gccaaccatt ctggtggacg ttccggccaa cgccaaagtg
     3721 tcgaaagaag agacgttcgg ccccctcgcc ccgctgttcc gctttaaaga tgaagccgat
     3781 gtgattgcgc aagccaatga caccgagttt ggccttgccg cctatttcta cgcccgtgat
     3841 ttaagccgcg tcttccgcgt gggcgaagcg ctggagtacg gcatcgtcgg catcaatacc
     3901 ggcattattt ccaatgaagt ggccccgttc ggcggcatca aagcctcggg tctgggtcgt
     3961 gaaggttcga agtatggcat cgaagattac ttagaaatca aatatatgtg catcggtctt
     4021 taactggaga acgcgaatga gcagcaataa agagttaatg cagcgccgca gtcaggcgat
     4081 tccccgtggc gttgggcaaa ttcaccccat tttcgctgac cgcgcggaaa actgccgggt
     4141 gtgggacgtt gaaggccgtg agtatcttga tttcgcgggc gggattgcgg tgctcaatac
     4201 cgggcatctg catccgaaag tggtggctgc ggtggaagcg cagttgaaaa aactgtcgca
     4261 cacctgcttc caggtgctgg cctacgagcc gtatctggag ctgtgcgaga ttatgaatca
     4321 gaaggtgcca ggcaattttg ccaagaaaac gctgctggtc accacaggtt ctgaagcggt
     4381 ggaaaacgcg gtgaaaatcg cccgcgccgc caccaaacgt agcggcacca tcgcttttag
     4441 cggcgcgtat cacgggcgca cgcattacac gctggcgctg accggcaagg tgaatccgta
     4501 ctctgcgggc atggggctga tgccgggtca tgtttatcgc gcgctttatc cttgcccgct
     4561 gcacggcata agcgaggatg acgctatcgc cagcatccac cggatcttca aaaatgatgc
     4621 cgcgccggaa gatatcgccg ccatcgtgat tgagccggtt cagggcgaag gcggtttcta
     4681 cgccgcgtcg ccagccttta tgcagcgttt acgcgctctg tgtgacgagc acgggatcat
     4741 gctgattgcc gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt ttgcgatgga
     4801 gcagatgggc gttgcgccgg atcttaccac ctttgcgaaa tcgatcgcgg gcggcttccc
     4861 gctggcgggc gtcaccgggc gcgcggaagt gatggatgcc gtcgctccag gcggtctggg
     4921 cggcacctat gcgggtaacc cgattgcctg cgtggctgcg ctggaagtgt tgaaggtgtt
     4981 tgagcaggaa aatctgctgc aaaaagccaa cgatctgggg cagaagttga aagacggatt
     5041 gctggcgata gccgaaaaac acccggagat cggcgacgta cgcgggctgg gggcgatgat
     5101 cgccattgag ctgtttgaag acggcgatca caacaagccg gacgccaaac tcaccgccga
     5161 gatcgtggct cgcgcccgcg ataaaggcct gattcttctc tcctgcggcc cgtattacaa
     5221 cgtgctgcgc atccttgtac cgctcaccat tgaagacgct cagatccgtc agggtctgga
     5281 gatcatcagc cagtgttttg atgaggcgaa gcagtagcgc cgctcctatg ccggaggcga
     5341 cgctgcgcgt cttgtccggc ctacggggat ccaggtcgga taaggcgttt acgccgcatc
     5401 cgacaatctg tacgtgaaca ggaagaaatc tatgttggcc gggtaaggcg gagccgctct
     5461 ccggcaaaaa gaatcaataa caattatacg cgtgacccga cgcgggaaat gtcggggcgc
     5521 tctcccaagt gacacacttt cgagaggatt caggatgggg caatcatcgc aaccacatga
     5581 gttaggcggc gggctgaagt cacgccacgt caccatgttg tctattgccg gtgttatcgg
     5641 cgcaagtctg tttgttggtt ccagcgtcgc catcgccgaa gcgggtccgg cggtattact
     5701 ggcctatctg ttcgccgggc tactggtggt tatgattatg cggatgttgg cggaaatggc
     5761 ggttgccacg cccgataccg gttcgttttc cacctatgcc gataaagcta ttggtcgttg
     5821 ggcgggttat accatcggct ggttgtactg gtggttttgg gtactggtta tcccgctgga
     5881 agccaacatc gccgccatga tcctgcactc atgggttcca ggcattccca tctggttatt
     5941 ttccctcgtc attaccctcg ccttaactgg cagtaactta ttaagcgtta aaaactacgg
     6001 cgaatttgag ttctggctgg cgctgtgcaa agtcatcgct atcctggcct ttattttcct
     6061 tggtgcagtc gcaattagcg gtttttaccc ttatgccgaa gtgagcggga tctcaagatt
     6121 gtgggatagc ggcggcttta tgcccaacgg tttcggtgcg gtattaagcg cgatgttgat
     6181 caccatgttc tcgtttatgg gcgcagaaat tgtcaccatt gccgccgcgg aatccgacac
     6241 gccggaaaaa catattgtcc gcgccaccaa ctcggttatc tggcgtattt ctatcttcta
     6301 tttatgttct atttttgtcg tcgtggcatt aattccgtgg aatatgcccg gactaaaagc
     6361 cgtcggttct tatcgctcgg tactggaatt gctcaatatt ccccatgcga aattgatcat
     6421 ggactgcgtg atattacttt ccgtaaccag ttgcctgaac tcggcgctgt ataccgcttc
     6481 caggatgctc tactccttaa gccgtcgcgg agatgcgcct gcggtaatgg gtaaaatcaa
     6541 ccgcagtaaa accccgtacg tggcggtgtt actctccacc ggcgcggcat tcttaacggt
     6601 ggtggtgaac tattacgcgc ctgcgaaggt atttaaattt ctgatcgaca gctccggcgc
     6661 tatcgccctg ctggtttatt tagtcatcgc cgtttcacag ttgcggatgc gcaaaattct
     6721 gcgagcagaa ggaagcgaaa ttcgcttgcg catgtggctt tatccgtggc tcacctggct
     6781 ggtcatcggc tttattacct ttgtgttggt agtgatgcta ttccgtccgg cgcaacagtt
     6841 agaagtgatc tccaccggct tattagcgat agggattatc tgtaccgtgc caattatggc
     6901 gcgctggaaa aagctggtat tgtggcaaaa aacacccgtt cataatacgc gctgatcatg
     6961 atcaggagtc acaccatgac cattacgtct ctggatggct atcgctggct gaagaacgat
     7021 attattcgcg gtaattttca accggatgaa aaattacgaa tgagtttgct gacatcgcgt
     7081 tatgcacttg gcgttgggcc gttacgggaa gctctttcgc aactggtggc ggaacggctg
     7141 gtcacggtgg tgaatcaaaa agggtatcgg gtggcgtcta tgtcagagca ggagctgctc
     7201 gatattttcg acgcccgcgc caatatggaa gcgatgttag tgagtctggc gattgcccgc
     7261 ggtggcgatg agtgggaggc agacgttctc gcaaaagcgc atctgctgag taagcttgag
     7321 gcctgtgacg ccagcgagaa aatgcttgat gagtgggatc tgcgtcatca ggcgtttcat
     7381 acggcaattg tggcgggctg tggttctcac tatttgctgc aaatgcgtga acggttgttt
     7441 gatctggcgg cgcgttatcg atttatctgg ctgcggcgaa cggtgctttc ggtggaaatg
     7501 ctggaggata aacacgatca gcaccagacc ctgactgcgg cggtactggc gcgagatacc
     7561 gcgcgcgcca gtgagttaat gcgccagcat ttactgacgc caattcccat tatccagcag
     7621 gcgatggctg gcaattaact actcttccgg aatacgcaac acttgccccg gataaatttt
     7681 atccgggctt tttagcatcg gtttattcgc ttcgaagatt ttattgtaca gattagcgtt
     7741 accgtagacc tgtttggaaa tggcactcag agtgtcgcca gacttaacgg tataaaactg
     7801 gctggcagtg gctggtgtcg ccgttttcac ctgatcatcg acactggcaa taccggaaat
     7861 attccccacc gcaacaagga ttttctcctt cgcctcctga ctcaggccgt caccagtgac
     7921 cgtcgctttg ccgtcggcaa tttgaatatt cactttatcg gcatccggta taccggtttt
     7981 gttcagatgc tcctgcacct tcttcgcctg atcgtcttta tcgtgctgac ctgtaaccgc
     8041 gtcccagagt ttttctccgg catctttcac aaaattgaac agacccatag ctacctcatt
     8101 cgttaacgga aacactcaga aagtgtagca gaggaacgcg gcgcgttaac gccgcgggga
     8161 tgagcacagg tttaatcgcg ggtttgcacc cagaacgcgt gaatcaaacc ggggatatac
     8221 ccaagcagtg tcaacagaat attaataatg aacgcccaac cgaacccttt accgagcagc
     8281 acgccgagcg gcggcagaat gatggtgatg acgattctcc agaaacccat atgtactccc
     8341 tataagaaaa ttactcattg tttaaaaaga gattttatct cttaagcgta gtaattttag
     8401 cggagcctgc cagtttcagc ttacgctt
//
LOCUS       DAFNBJ010000191         8391 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000191 DAFNBJ010000000
VERSION     DAFNBJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.191"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            4..894
                     /gene="alsR"
                     /locus_tag="L2X49_004278"
                     /gene_synonym="rpiR"
     CDS             4..894
                     /gene="alsR"
                     /locus_tag="L2X49_004278"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HBN4799273.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            953..1888
                     /gene="alsB"
                     /locus_tag="L2X49_004279"
     CDS             953..1888
                     /gene="alsB"
                     /locus_tag="L2X49_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HBN4799274.1"
                     /translation="MNKYLKYFSSTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            2015..3547
                     /gene="alsA"
                     /locus_tag="L2X49_004280"
     CDS             2015..3547
                     /gene="alsA"
                     /locus_tag="L2X49_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HBN4799275.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNISYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHETVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            3526..4506
                     /gene="alsC"
                     /locus_tag="L2X49_004281"
     CDS             3526..4506
                     /gene="alsC"
                     /locus_tag="L2X49_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HBN4799276.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            4517..5212
                     /gene="alsE"
                     /locus_tag="L2X49_004282"
     CDS             4517..5212
                     /gene="alsE"
                     /locus_tag="L2X49_004282"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HBN4799277.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            5196..6125
                     /gene="alsK"
                     /locus_tag="L2X49_004283"
     CDS             5196..6125
                     /gene="alsK"
                     /locus_tag="L2X49_004283"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HBN4799278.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            6397..8382
                     /gene="yjcS"
                     /locus_tag="L2X49_004284"
     CDS             6397..8382
                     /gene="yjcS"
                     /locus_tag="L2X49_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HBN4799279.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         2185 a         2048 c         2131 g         2027 t
ORIGIN      
        1 gtgatgagcc agtcagagtt tgattcagcg cttccgaacg gtatagggtt agcgccttac
       61 ctgcgaatga aacaggaagg aatgacagaa aatgaaagcc gcatcgtgga gtggttactc
      121 aaacccggta acctgagttg tgcacccgca attaaagatg tcgcagaagc tctggcggta
      181 tctgaagcga tgatagttaa ggtatcaaag ctgctggggt ttagcggctt tcgtaactta
      241 cgcagtgcgc tggaagatta tttttctcag tcagaacaag tattgccttc cgagttggct
      301 tttgatgaag cgccgcagga tgtggtgaat aaggtattta acatcacttt acgcaccatt
      361 atggaaggtc agtcgatcgt caacgttgat gagatccacc gtgccgcccg ctttttctat
      421 caggccagac agcgggattt atacggtgcc ggaggatcaa atgctatctg tgctgatgta
      481 cagcacaagt tcttgcgcat tggcgtacgc tgtcaggcct atccggacgc tcacatcatg
      541 atgatgtccg cttcgttgtt acaggaagga gatgttgtgc tggtagtgac ccattccggg
      601 cgaaccagtg atgtaaaagc ggccgtagaa ctggcaaaaa agaacggggc aaagattatt
      661 tgtataacgc atagctacca ttcaccaata gcgaaactgg ccgattatat tatttgctca
      721 ccagccccgg aaacgccgtt attaggtcgt aatgcctcgg caagaatatt acaactaact
      781 ttgctggacg ctttttttgt ctctgtcgcc cagctcaaca ttgaacaagc taatattaat
      841 atgcaaaaaa ccggcgcaat tgttgatttc ttctcaccag gcgcgctgaa ataagaaaaa
      901 ggtaattccg ccatcgcgga gttacctgtc cccttaccgt gagaacaata ttatgaataa
      961 atatctgaaa tatttcagca gcacactcgt gggcttaatg ttgtcaacca gcgcttttgc
     1021 tgccgccgaa tatgctgtcg tattgaaaac cctctccaac ccattttggg tagatatgaa
     1081 aaaaggcatt gaagatgaag caaaaacact gggcgtcagc gttgatattt ttgcctctcc
     1141 ttcagaaggc gattttcaat ctcaattgca gttatttgaa gatctcagta ataaaaatta
     1201 caaaggtatc gccttcgctc cattatcctc agtgaatctg gtcatgcctg tcgcccgcgc
     1261 atggaaaaaa ggcatttatc tggttaatct cgatgaaaaa atcgacatgg ataatctgaa
     1321 aaaagctggc ggcaatgtgg aagcttttgt caccaccgat aacgttgctg tcggggcgaa
     1381 aggcgcgtcg ttcattattg acaaattggg cgctgaaggt ggtgaagtcg caatcattga
     1441 gggtaaagcc ggtaacgcct ccggtgaagc gcgtcgtaat ggtgccaccg aagccttcaa
     1501 aaaagcaagc cagatcaagc ttgtcgccag ccagcctgcc gactgggacc gcattaaagc
     1561 actggatgtc gccactaacg tgttgcaacg taatccgaat attaaagcga tctattgcgc
     1621 gaatgacacg atggcaatgg gtgttgctca ggcagtcgca aacgccggaa aaacgggaaa
     1681 agtgctggtc gtcggtacag atggcattcc ggaagcccgc aaaatggtgg aagccggaca
     1741 aatgaccgcg acggttgccc agaacccggc ggatatcggc gcaacgggtc tgaagctgat
     1801 ggttgacgct gagaaatccg gcaaggttat cccgctggat aaagcaccgg aatttaaact
     1861 ggtcgattca atcctggtca ctcaataaaa ctgaatgatg ccggatgatg atgctaatga
     1921 agtgtcttat ccggccaaca aaataacctg aacgtagacc tgataaatgt agccatcagg
     1981 caataatacg atatttcgtg cagagaggtt atttatggcc acgccatata tatcgatggc
     2041 ggggatcggc aagtcctttg gtccggttca cgcattaaag tcggttaatt taacggttta
     2101 tcctggtgaa atacatgcat tactaggaga aaatggcgcg ggtaaatcca cgctaatgaa
     2161 agttttatcc ggaatacatg agccgaccaa aggcaccatt accattaata acattagcta
     2221 taacaagctg gatcataaat tagcggcaca actcggtatc gggattattt atcaggaact
     2281 cagcgttatt gatgaattaa ccgtactgga aaatttatat attggtcgtc atctgacgaa
     2341 aaaaatctgt ggcgtcaata ttatcgactg gcgagaaatg cgtgtccgcg ccgccatgat
     2401 gttattacgc gtgggcttga aagttgatct agatgagaaa gtggcgaatt tatctatcag
     2461 ccacaagcag atgctggaaa ttgccaaaac gctgatgctc gatgccaaag tcatcatcat
     2521 ggatgaaccc acctcctcac tcaccaataa agaggtggac tatctgtttc tgatcatgaa
     2581 tcagttgcgt aaagagggta cggccatcgt ctatatctcg cataagttgg cggaaattcg
     2641 ccgtatttgc gaccgctata cggtgatgaa agacggtagc agcgtttgca gcggcatggt
     2701 gagcgatgtg tcaaatgacg atatcgtccg tctgatggta ggccgcgaac tgcaaaaccg
     2761 ttttaacgcg atgaaggaga atgtcagcaa ccttgcgcac gaaacggttt ttgaggtgcg
     2821 caacgtcacc agccgtgaca gaaaaaaggt ccgggatatc tcatttagcg tctgccgggg
     2881 agaaatatta ggctttgccg ggctggtcgg ttccggacgt actgaactga tgaattgtct
     2941 gtttggcgtt gataaacgcg ctggcggaga aatccgtctt aatggcaaag atatctctcc
     3001 acgttccccc ctggatgccg tgaaaaaagg gatggcttac atcactgaaa gccgccggga
     3061 taacggtttt ttccccaact tttccatcgc tcagaacatg gcgatcagcc gcagtctgaa
     3121 agacggcggc tataaaggcg cgatgggctt gtttcatgaa gttgacgagc aacgtaccgc
     3181 tgaaaatcaa cgcgaactgc tggcgctgaa atgtcattcg gtaaaccaga atatcaccga
     3241 actctccggg ggaaatcagc agaaagtcct gatctccaaa tggctgtgct gttgcccgga
     3301 agtgattatt ttcgatgaac ctacccgcgg catcgacgtt ggcgcgaaag ccgaaattta
     3361 caaagtgatg cgccaactgg cggacgacgg aaaagtcatc ctgatggtgt catctgaact
     3421 acctgaaatt atcaccgtct gcgaccgcat cgccgtgttc tgcgaaggac gactgacgca
     3481 aatcctgacg aatcgcgatg acatgagcga agaggagatt atggcatggg ctttaccaca
     3541 agagtaaaaa gcgaagcgag cgagaagaaa ccgttcaact ttgcgctgtt ctgggataaa
     3601 tacggcacct tttttatcct ggcgatcatc gtcgccattt ttggttcgct gtcgccagaa
     3661 tattttctga ccaccaataa tattacccag atttttgttc aaagctccgt gacggtattg
     3721 atcggcatgg gcgagttttt cgctatcctg gtcgctggta tcgacctctc ggttggcgcg
     3781 attctggcgc tttcgggtat ggtgaccgcc aaactgatgt tggcaggtgt tgacccgttt
     3841 ctcgcagcga tgattggcgg tgtactggtt ggcggcgcac tgggggcgat caacggctgc
     3901 ctggtcaact ggacggggct acacccgttc atcatcaccc ttggcaccaa cgcgattttc
     3961 cgtgggatca cgctggtgat ctccgatgcc aactcggtat acggcttctc ctttgacttc
     4021 gtgaacttct ttgccgccag cgtaattggg atacctgtcc ccgttatctt ctcgctaatt
     4081 gtcgcgctca tcctttggtt tctgacaacg cgtatgcggc tcgggcgcaa catctacgca
     4141 ctgggcggca acaaaaattc ggcgttctat tccgggattg acgtgaaatt ccacatcctg
     4201 gtggtgttta tcatctccgg tgtttgtgca ggtctggcag gcgtcgtctc aactgcacga
     4261 ctcggtgccg cagaaccgct tgccggtatg ggttttgaaa cctatgccat tgccagcgcc
     4321 atcattggcg gcaccagttt cttcggcggc aaggggcgca ttttctctgt ggtgattggc
     4381 gggttgatca tcggcaccat caacaacggt ctgaatattt tgcaggtaca aacctattac
     4441 caactggtgg tgatgggcgg attaattatc gcggctgtcg cccttgaccg tcttatcagt
     4501 aagtaaggaa ttgaacatga aaatctcccc ctcgttaatg tgtatggatc tgctgaaatt
     4561 taaagaacag atcgaattta tcgacagcca tgccgattac ttccacatcg atatcatgga
     4621 cggtcacttt gtccccaatc tgacactctc accgttcttc gtaagtcagg ttaaaaaact
     4681 ggcaactaaa ccgctcgact gtcatctgat ggtgacgcgg ccgcaggatt acattgctca
     4741 actggcacgt gcgggagcag attttatcac tctgcatccg gaaaccatca acggccaggc
     4801 gttccgcctg attgatgaaa tccgccgtca tgacatgaaa gtggggctga tccttaatcc
     4861 ggagacgcca gttgaggcca tgaaatacta tatccataag gccgataaaa ttacggtcat
     4921 gactgtcgat cccggctttg ccggacaacc gttcattcct gaaatgctgg ataaacttgc
     4981 cgaactgaag gcatggcgtg aacgagaagg tctggagtac gaaattgagg tggacggttc
     5041 ctgcaaccag gcaacttacg aaaaactaat ggcggcagga gcggatgtct ttatcgtcgg
     5101 cacttccggc ctgtttaatc atgcggaaaa tatcgacgaa gcatggagaa ttatgaccgc
     5161 gcagattctg gctgcaaaaa gcgaggtaca gcctcatgca aaaacagcat aacgtcgtag
     5221 cgggcgtgga tatgggggca acgcatatcc gcttttgtct gcggacagca gaaggtgaaa
     5281 cgctacactg cgaaaaaaag cggaccgcag aagtcattgc tcccggcctg gtgtcgggta
     5341 tcggcgaaat gattgacgag caactcagac gctttaacgc tcgctgtcat ggtctggtga
     5401 tgggatttcc ggcgctggtc agtaaagata aacgcaccat tatttctacg cctaacctgc
     5461 cgttaacagc ggcggattta tatgatctcg ccgataagct cgaaaatacg ctgaattgtc
     5521 cggttgagtt ttcccgcgac gttaacctgc aactctcctg ggacgtagta gaaaaccgcc
     5581 ttacgcaaca actggttctg gcggcctatc tcggtacggg gatggggttc gcagtgtgga
     5641 tgaacggtgc gccgtggacg ggtgcacacg gtgtggcagg cgaactgggt catatccccc
     5701 tgggagatat gacccaacac tgcgcgtgtg gcaatcctgg atgcctggaa accaattgct
     5761 ctggaatggc gctaagacgc tggtacgaac aacagccccg aaattaccca ttgagcgatc
     5821 ttttcgtcca tgcggaaaac gctcctttcg tccagagtct gcttgaaaac gcggctcgag
     5881 ccattgccac cagcattaat ctgttcgatc ccgatgcggt gattctgggc ggtggcgtga
     5941 tggatatgcc cgcctttcca cgcgagactc tcattgccat gacccaaaag tacctgcgcc
     6001 gtccactgcc gcatcaggtc gtgcgcttta ttgccgcctc atcttctgac tttaatggcg
     6061 ctcagggggc agcaatattg gcgcatcaac gttttttgcc acagtcctgt gctaaagtcc
     6121 catgagtaaa gcgttggcac agtagcgcaa tgtcccgctg tgccaacgac aggtacaata
     6181 tatataataa atacataaaa cggtgcatat tatttccagc aatgatagtt ctactgtaat
     6241 tccccccttc agcacaaatg cctaataaaa tggatcgttc cgctcacact tccacttatt
     6301 aaagtcgcaa tttcccaatt atgataacgc cagctttaaa tgacgtattt tgcatggatt
     6361 acatatttac gacataaatg ataaatggag atttatatga ataactctcg gttattccgt
     6421 ttgagcagaa ttgttattgc gttaactgcc gccagcggca tgatggtaaa taccgcttac
     6481 gcaacagatg aagcgaaagc cgccactcaa tatacccaac aggttaatca gaattacgcc
     6541 aaatcattac cgtttagcga tcgtcaggat tttgacgacg ctcagcgtgg atttatcgct
     6601 ccgctgctgg atgaaggtat tctgcgcgat gctaatggca aaccatatta tcgcggggaa
     6661 gattataaat ttgatatcaa tgcgcccgcg cccgaaaccg ttaaccctag cctgtggcgt
     6721 cagtcgcagt taaacggtat ttccggcctg tttaaagtca ccgacagaat gtaccaggtt
     6781 cgcggtcagg atatctccaa catcaccttt atcgaaggtg ataccgggat tattgtcatc
     6841 gacccgctgg tgacgccacc gagcgcaaaa gccgcccttg acctttactt ccaaaatcgc
     6901 ccgcaaaaac cgattgttgc ggttatttat acccacagcc ataccgacca ctatggcggc
     6961 gtgaaaggca ttatctctga agctgatgtg aaatccggta aagtgcaggt tatcgcccct
     7021 gctggcttta tggacgaagc catcagcgaa aacgtactgg cgggtaatat tatgagccgc
     7081 cgtgcacttt actcctacgg cctgctgctg gcgcataacc ctcagggtaa catcggcaat
     7141 ggtcttggcg taacgctggc atcgggctac ccgagcatca tcgcaccgaa caaaaccatc
     7201 accaaaaccg gtgagaagat gattatcgac ggcctggagt ttgacttcct gatgacccca
     7261 ggtagcgaag caccagccga aatgcacttc tatattccgg ccctgaaagc gctgtgtacc
     7321 gccgagaacg ccacgcatac cctgcacaac ttctacactc tgcgcggcgc gaaaacccgc
     7381 gacaccagca agtggaccga gtatctgaac gaaacgctgg atatgtgggg taacgacgcg
     7441 gaagtcctgt ttatgccgca cacctggccg gtctggggca ataagcatat caatgattat
     7501 attggtaaat atcgcgatac tatcaagtac attcacgacc agaccctgca cctggcgaac
     7561 cagggctaca ccatgaatga aatcggcgac atgattaaac tgccgcctgc acttgccaat
     7621 aactgggcca gccgtggcta ttacggttct gtcagccaca acgcccgcgc ggtgtataac
     7681 ttctatcttg gctattacga cggtaacccg gctaacctgc atccgtatgg tcaggtggag
     7741 atgggtaaac gttacgtgca ggcgctgggc ggttctgccc gtgtcatcaa cctggcgcaa
     7801 gaagcgaaca agcaaggtga ttaccgctgg tcggcagaac tgctgaaaca ggtgattgcc
     7861 gccaacccgg gtgaccaggt cgcgaagaat ctgcaagcga ataactttga acagctgggc
     7921 tatcaggccg agtccgccac ctggcgcggt ttctacctga ccggcgcgaa agagctgcgc
     7981 gaaggggtgc ataagttcag ccacggcacc accggttccc cggacaccat tcgcgggatg
     8041 tcggtcgaaa tgctgttcga ctttatgtcc gttcgcctcg atagcgcgaa agccgcgggt
     8101 aaaaatatca gcctgaactt caatatgagc aatggcgata acctcaacct gacgctgaac
     8161 gatagcgtgc ttaactaccg taaaacactg caatcccaag ctgacgcctc tttctacatc
     8221 agccgtgaag atctgcacgc cgtgctgacc gggcaagcca aaatggcgga tctggtaaaa
     8281 gcgaagaaag ccaaaattat tggcaatggc gcgaaactgg aagaaattat cgcctgtctg
     8341 gataatttcg atttgtgggt gaatatcgta accccaaatt aatgtcactc g
//
LOCUS       DAFNBJ010000192         8273 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000192 DAFNBJ010000000
VERSION     DAFNBJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.192"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            196..966
                     /locus_tag="L2X49_004285"
     CDS             196..966
                     /locus_tag="L2X49_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBN4799280.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            957..1430
                     /locus_tag="L2X49_004286"
     CDS             957..1430
                     /locus_tag="L2X49_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBN4799281.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1537..2076
                     /gene="dnaT"
                     /locus_tag="L2X49_004287"
     CDS             1537..2076
                     /gene="dnaT"
                     /locus_tag="L2X49_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBN4799282.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2079..2816
                     /gene="dnaC"
                     /locus_tag="L2X49_004288"
     CDS             2079..2816
                     /gene="dnaC"
                     /locus_tag="L2X49_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBN4799283.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2865..3359
                     /locus_tag="L2X49_004289"
     CDS             2865..3359
                     /locus_tag="L2X49_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBN4799284.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3613..5904
                     /gene="opgB"
                     /locus_tag="L2X49_004290"
     CDS             3613..5904
                     /gene="opgB"
                     /locus_tag="L2X49_004290"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBN4799285.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6044..7066)
                     /gene="lgoD"
                     /locus_tag="L2X49_004291"
     CDS             complement(6044..7066)
                     /gene="lgoD"
                     /locus_tag="L2X49_004291"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HBN4799286.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7205..8119
                     /locus_tag="L2X49_004292"
     CDS             7205..8119
                     /locus_tag="L2X49_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN4799287.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
BASE COUNT         2093 a         2146 c         2013 g         2021 t
ORIGIN      
        1 atgatgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc tttttatcac
       61 cgagcttctc ccgataacca ggattaatca gcgcaatttt gatcttaacc taccgcaaaa
      121 caaccgtgct tcatgataca aatggattct cattatctga acaatacgca gcacgtctat
      181 gacaaagggc gagttatgca aactgagcaa cagcgagccg taacacggct ttgtatccag
      241 tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca
      301 cgactgggtc gggcgctggg aatggacagt gtcgaaagtt ctatctcttc gaacgccata
      361 gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc
      421 ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc ggagcatcat
      481 ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcagccatt acgttaccca
      541 agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa actcaataac
      601 ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat gtatatccgc
      661 cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac cgctttcgcc
      721 gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac ccccaccatt
      781 gcgatggccg ccagcgttct gctgctggtg ccgggctttc cgttgattaa tgccgtcgcc
      841 gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg
      901 acactggcta cctgcgtcgg cgtggtgatg gcactgacga tttgggggct acgcggatgg
      961 gtgtgatcga atttctgttt gcattggcgc aggatatgat cctcgccgcc attcctgcgg
     1021 tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt gcgctgcttg
     1081 gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat attgagtggt
     1141 caacctttat ggcttctatg ctggtcggca ccattggtat tcaatggtcg cgctggtatc
     1201 tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg
     1261 cttataccgc aatgatttcg gcggtaaaaa tcagccagct aggttacagc gaaccgttga
     1321 tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg
     1381 gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg
     1441 cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct
     1501 cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg
     1561 gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa
     1621 ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca
     1681 cgcctggctg aactgctggc gctggaagaa aagctggcgc gtccgggaag cgatgtcgct
     1741 ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa
     1801 ttcgccatgt atccggactg gcaacccgat gccgacttta tccgcctggc ggcgctatgg
     1861 ggcgtggcgc taagagagcc ggtaaccacc gaagaactgg cctcattcat tgcctactgg
     1921 caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg
     1981 caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagtgaa
     2041 cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga
     2101 tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc cgcattcaaa acgggtgaag
     2161 aactactggc ctggcaaaaa gaacaagggg cgatccgctc cgccgctctc gaacgtgaaa
     2221 atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgtcca ctgcatcaga
     2281 actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag
     2341 cgcgccagta tgtcgaagag tttgacggca acatcgccag ctttatcttt tctggtaagc
     2401 caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta
     2461 aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga
     2521 atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat ctgctggtga
     2581 tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg
     2641 tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag
     2701 agatgacaaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttgt
     2761 gggtgatctt caactgggat agctaccgta gccgggtaac gggtaaagag tattaatccc
     2821 cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactatta
     2881 aacaccttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg
     2941 cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga
     3001 cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc ggaaaccagg
     3061 ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc gcgaagcaaa
     3121 agctggcttc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc
     3181 tgaacagtga agagcaaaaa gaagacacca actatctgga cggtattcag ggtttgctga
     3241 aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacaactccg ctggcagaaa
     3301 aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa
     3361 ccgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct gcccttttcc
     3421 ctgctcctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa aaaagtctgt
     3481 ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca gcaatatggc
     3541 gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc ccggtttagt
     3601 gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc tgtgctgatt
     3661 tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacattaac ggtgctgggg
     3721 ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg cgatggtatt
     3781 aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt cagcaaatac
     3841 atcctgccgg gtatcggcat tgtgctgggg ctgacagcgg tgttcggtgc gctgggctgg
     3901 atcctgcgcc gtcgtcgcca tcatccgcac cattttggtt acagcctgct ggcgctctta
     3961 ctggcgctgg gttcggtgga cgccagccca gcatttcgtc agataacgga actggtgaaa
     4021 tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc gtcgaaaact
     4081 atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga gcggacctat
     4141 tttgataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa aaatgaaggc
     4201 ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc gggcatggtg
     4261 gcttctcagt gcggcatacc gctgtttgcc ccctttgaag gcaacgcctc cgcctctgtc
     4321 tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc gggttatcag
     4381 aactatttcg tgcagggcgc gaatctgcgt tttgccggta aagatgtgtt cctgaagtcg
     4441 catggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtggc cgacccgcac
     4501 tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg gaaaaagttt
     4561 gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt cgatacccat
     4621 cacccggatg gttttatctc gcgtacctgt aaccgcaaaa aatatgattt tgacggtaag
     4681 ccgaaccagt cattcagcgc ggtaagttgc agccaggaga atatcgcggc gtttatcaac
     4741 aaaatcaaag cgtcaccgtg gtttaaagat accgtcatcg tcgtctcttc tgaccattta
     4801 gcgatgaaca acacggcgtg gaaatacctt aataagcagg atcgcaataa cctgtttttt
     4861 gtcattcgtg gcgacaagcc gcagcaagag acgctggcag tgaagcgtaa cacgatggat
     4921 aacggcgcga cggtgctgga cattctcggt ggcgataact atctcggact tggtcgtagc
     4981 agtttatccg ggcagtcaat gtcggaaatc ttcctcaata tcaaagagaa aacattggcg
     5041 tggaagccgg atatcatccg cctgtggaaa ttccctaaag agatgaaaga gttcaccatc
     5101 gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc gctgctgttg
     5161 cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg aatactcagc accgctgcgt
     5221 ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgaccg ttgctataag
     5281 atggcacaac tctgggctcc ggaactggca ctctccaccg actggtgtgt ctcgcaaggg
     5341 cagcttggcg gtcagcaaat tgttcagcat gttgacaaaa caacatggca gggcaaaacg
     5401 gcatttaaag atacggtcat cgacatggcg cgttataaag gcaatgtcga tacgctgaaa
     5461 attgttgata acgatattcg ctacaaagcc gacagtttca tcttcaacgt cgccggtgcg
     5521 ccagaagagg tgaaacagtt tagtgggatt tcccgtccag agtcgtgggg ccgctggtcc
     5581 aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc gaagaaattt
     5641 gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat tccggtacgc
     5701 gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag tgaccaccac cacactgcat
     5761 ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc tgtctcaacc
     5821 aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat ggtggaaatt
     5881 aaagttgtgg aacgtgaagg gtaatttctg actgatgtgt aggtcgcata aggtgcgacc
     5941 tacacatctt ctccggccta caaaacatca ttgagaaaca gaaaaaaaac agcgcccaac
     6001 acttccccgg acgctgcatg gcaactttaa taatctcgcc ttatcagaaa gtaattacac
     6061 ccttaatcaa ctcacgattg ttaatcacat cgcgctcgta ggtttctgcc agcgtggcga
     6121 acggatagcg atgggttaac atcatgtcgg cagtgatttt tccttccgcc atcagtcgac
     6181 cgactttagc aaaatcttcc ggcgtggcgt tgcggctgcc catcatcgtc gtttcttttt
     6241 tatggaattc cggatcggag aactgcaact cacctttaaa caggccgaca aataccaccg
     6301 tgccgccgtg acgaatcaaa ttcacggtgt tattcatcgc atgttgatta cctgtcgcgt
     6361 cgatcacttt ctgcgccagc gaaccaccaa actgcgcccg tagctgcgcg tcaaaatctt
     6421 cggctgacgg gtccagtaaa ggtaattcca gacgcgttgc cacatgttca cggcgcgccg
     6481 gactggtatc cgccaccacc acctgtgcgc catcggcttt agcgattgcc gccgcgccca
     6541 gaccgattgg ccccgccccg accaccagca cctgctcgcc gggagcaatg gctgcgcgac
     6601 gcaccgcatg agcgctaata gcgaaaggtt caatcaatgc ggccgcctgc gggtcaatac
     6661 cgtctgcggg caaaatgttc gccaccggca cgctcaaata ctcactaaaa ccgccatcct
     6721 gatgcacgcc aatgactgaa attttttcac agcaattggt acgcccgctt ttacacgccg
     6781 ggcattgctg acaggcaaca taagggatca cagcaacttg ctgaccattt ttaagatcag
     6841 caatattttt acccagccca acaatctccc cacatatttc atggcctaaa acacgtggat
     6901 aactaaaaaa tggttgattt ccaccccagg catgaatatc ggtaccgcaa atcccgacag
     6961 acttaatttt tattaatgct tcattgtcac ccggaatagg tatctcgcgt tgtttccaga
     7021 ctaattcttt cggctgctgg caaattaaaa cattcatcgt agacatgatt cggctctcca
     7081 ttttttgttg ccttagatat caatgaaaat gaaaaatggc agcggaacgg aaaatctgtt
     7141 ttgtaaaaac acacgcataa aaatgtttta aatcgggttt taatggactc acaaggagga
     7201 aaatatgagt cgttcacaaa atttacgcca caatgtgatt aaccaggtca ttgatgatat
     7261 ggcgcggggt catatccctt ccccacttcc atcgcaaagt gcgctggcgg aaatgtacaa
     7321 catcagccgc acaacagtgc gtcatattct cagccactta cgcgaatgcg gcgtcttaac
     7381 gcaggttgga aacgactatg ttattgcgcg taaacctgac catgatgacg gtttcgcctg
     7441 tactaccgct tcaatgagtg aacaaaacaa agtgtttgag caggcttttt tcacgatgat
     7501 aaaccagcgt cagttacgcc ctggggaaac tttttctgaa ctgcaactgg ctcgggccgc
     7561 gggtgtcagc ccggtggttg taagagaata tcttttaaaa ttcggccgtt ataatcttat
     7621 tcaaagcgaa aaacgcggcc agtggagcat gaagcaattc gatcaatcct acgccgagca
     7681 gctgtttgag ctacgcgaaa tgctcgaaac acattcactt cagcactttc tcaacctgcc
     7741 tgatcatgat ccccgctggc tgcaggcgaa aaccatgctc gaacgtcatc ggctactgcg
     7801 tgacaacatc ggcaatagct ttcgcatgtt ctcgcaactt gacagagatt ttcactcctt
     7861 actgctttca gctgccgaca atatcttttt tgatcaatcg cttgagatca tctcagtcat
     7921 ctttcatttt cactatcaat gggacgaaag cgatcttaaa cagcgcaata tcattgccgt
     7981 cgatgagcat atgaccattc tcagcgctct gatttgccgc agtgaccttg atgccaccct
     8041 tgcgctccgt aaccatttga attcagctaa acaatcaatg attcgatcca ttaatgagaa
     8101 cacgcgttat gctcattaag tacggattaa aaacagcaaa ctattagcgg gtgcacggtt
     8161 ttatcaaaag ataaataccg ggaaattccc gcttacctat gctcacaatc agtcgactaa
     8221 aaattttttt gtgagcggta gtaaagtcct aaaactttaa cctgccgtta atg
//
LOCUS       DAFNBJ010000193         8225 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000193 DAFNBJ010000000
VERSION     DAFNBJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.193"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(190..801)
                     /locus_tag="L2X49_004293"
                     /pseudo
     CDS             complement(190..801)
                     /locus_tag="L2X49_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSen4 family transposase"
     gene            complement(963..1307)
                     /locus_tag="L2X49_004294"
     CDS             complement(963..1307)
                     /locus_tag="L2X49_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799288.1"
                     /translation="MELGIMVCIVLLLSLAAVWISNKTVGLLEIHYFKRPLSMEYAAW
                     LRVMCAGLLFIFIQMVPAFTFLYTLKLVALLNLGVQTMKLNGVYKFKRTGMMPPKDAP
                     HDLGRFNPFRKK"
     gene            complement(1243..2151)
                     /locus_tag="L2X49_004295"
     CDS             complement(1243..2151)
                     /locus_tag="L2X49_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799289.1"
                     /translation="MQWISTTSQKLDERLYRVCVWVKKYHEDAINRVVLTVDLSKARH
                     DPIEDQVRAELLCGHYFLMRKENNAKSAPADQNFEFLPDGRNLSWQTSTPGLQHLLLN
                     SNVPESLRPLADYIQMRLAKLTMVPMSGTIMKAALEDSISWVKVDLRYYWQYELVNSH
                     LGPVQVTHKALVRFGRLAKHDENASAIRMLRQRLSSPFIQEFEMPGEELKRKQKIMNT
                     VDVKMLFHTHYPGQKLLLARYANGWVLVDCFLFHHIKPKKKAKNKTKAGKSETQNIKS
                     TGSEDHGTGHYGVHRVVTLTGSGVDQ"
     gene            complement(2162..3121)
                     /locus_tag="L2X49_004296"
     CDS             complement(2162..3121)
                     /locus_tag="L2X49_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBN4799290.1"
                     /translation="MASEDLLFYRDRILERIEKLRSFLNDHQPVMAELMTVGTVTRHE
                     ERLKEVNPIEVSTISDASVDEIIRVIQDFCIDDIDVLSRNSTKVTTKYPGLIIVPEGA
                     EQLSGIICDINDAKSDFASAMKRVNSEKHIRFKEVHRKLPGLVTAHSTRKILFVEEQL
                     KKVTFAWRLNRNQVKTNASDLIAMLDKRRLVAVKSPVTTDLTVVANIDRAKALLERKV
                     LRDGEGYRLCRTNTFPVPIAHLFTYRPEGKERGSSKYAETDYKVVKASLPIFGVGQKP
                     TIKRLQDWVPAQDEALSNGRRSNHSYTELVPGADLGIFIMRKV"
     gene            complement(3210..3485)
                     /locus_tag="L2X49_004297"
     CDS             complement(3210..3485)
                     /locus_tag="L2X49_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799291.1"
                     /translation="MHLLVEGKGVIPDVSVSDLGNVQNIRNELIGLDWWVKFGVKQDW
                     QRINLQRQPVSFGLLNDPNRCFRHDGQQVEPLPLYTTGNPGELRKVL"
     gene            complement(3703..4095)
                     /locus_tag="L2X49_004298"
     CDS             complement(3703..4095)
                     /locus_tag="L2X49_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799292.1"
                     /translation="MVFWIIISFMFLSAMLMTGHLLNKVVLQPVWLCIWSLPWLGFLL
                     KVRVTEMDKGLVNTLQLGDLPVFRWSRSVVPGTWSVDRINGRYRLNRIGTDGLTVHYP
                     WRLNYQPLFLTPDKQISDINGREHERKF"
     gene            complement(4175..4711)
                     /locus_tag="L2X49_004299"
     CDS             complement(4175..4711)
                     /locus_tag="L2X49_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799293.1"
                     /translation="MKSKIHSSGTSGTKRVLKTDIALPLLCWVFTSPFSNWTDKFFTG
                     TEVPEGSLPGLEQAPEAIFRFVLNDEGFDVGFDAVGMDLCCFSIPLSTMPTKNLDDEE
                     TLSRLTGDVIHGVLLSLPEYIEMPDRLVYQLTDEVMAFNSHCGNGILHGWTTAQELWR
                     NEILPRTTILMQQTSVIH"
     gene            complement(4785..5042)
                     /locus_tag="L2X49_004300"
     CDS             complement(4785..5042)
                     /locus_tag="L2X49_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799294.1"
                     /translation="MSNTDSQTVPADALCENSSQVKIQLSRSASRALTTAKEILETRG
                     LVSVSTEDLVQACLEENQPLDLASIYLEHRLKQKGTSICEF"
     gene            complement(5050..5640)
                     /locus_tag="L2X49_004301"
     CDS             complement(5050..5640)
                     /locus_tag="L2X49_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799295.1"
                     /translation="MMTESFFDWVVYCITGVILASAFLEGKRTRFPAAFFYGSIVLLV
                     VLIVAGALNWWFYRSKFGSVVVIFAATTLLMTRFRLVIYAFRFRTLVADLQSHVAKGF
                     RIILVLPENEDEKTVLLSKLSKVIHSGTLFYTRTALGPYSGDLLHALGQKHRNGEGYL
                     LLCEQQLPARTWLSTVENGQPEKSIAVNFHSIPDME"
     gene            complement(5695..5964)
                     /locus_tag="L2X49_004302"
     CDS             complement(5695..5964)
                     /locus_tag="L2X49_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799296.1"
                     /translation="MPATTILSNNPVNSVFSNDNSHFTYRHIDFSIELLLSSGTDSQL
                     EYLFLKPAAEVKIDLQRRLASGERYVTTESCDNFSEVDGCMGHDC"
     gene            complement(6377..7333)
                     /locus_tag="L2X49_004303"
     CDS             complement(6377..7333)
                     /locus_tag="L2X49_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799297.1"
                     /translation="MTNSTDNQNYVRAVLAGIGIDFDETEMFISVSHCQSDEVSFTCS
                     ISASELRESAGHYVDTLNDTQLAGLDADALKKRLVYFLEVFDLVSGQYLDISGKHFAT
                     SRFEYDDVCSEILSNSADSAQPGGYDREEYKRLMEVDGQVLIARFALEKFWDTHFIGL
                     INYVSDEITSGLYEVYRTFSDINMAGYTFSEYSYTRRITDELSLHISLKEDDFEEQLT
                     DCYMDETTLPSGKVVLRRNNESIIGIYEGYASKSYFPMVANVRVLDTDGEVVTELYQG
                     VNVSELAGGRIKIHDRQELISEVFANLREFIPASEDKIFDAA"
     gene            complement(7510..>8225)
                     /locus_tag="L2X49_004304"
     CDS             complement(7510..>8225)
                     /locus_tag="L2X49_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799298.1"
                     /translation="LRTKIKYFLKVYEKSGFQNIRIRGYHNAHSTIDIVDIASLILAG
                     SVPESEHDSIDPVLRKEIYQNRMSVEGKVLIARFALKQFFHSDFGDFILEFEKSISKC
                     LNTSLQIIKSVKNSFNRLGQYQYQRRVKDDLTLHLDLNTDEYPTCMPDLYIGFKESEG
                     TTGVYRDDEKIIRLYTGVSSGKDVPVMMTVRFTGCDGSVLSESSHGTYCSVGPTGRVQ
                     VCDRVALVQEAVEELRDVV"
BASE COUNT         2280 a         2059 c         1759 g         2127 t
ORIGIN      
        1 tcctgaatac tccaagctca ttaatcaagg tatcgtgcct gaaaaactaa gtacgggggc
       61 tattcacttc cacgaaacgg caaagagcga cattgctcaa gcctgttgtg agttgtatca
      121 gcaatcacct gaaaatagtc gcgtcatggc accgaccaag tgacctgccc ccacgattag
      181 atacaacact cagttagtaa cgtcggaatc ttcattctca gaatgaccct ttctccagcc
      241 cgctgcaaat tcagacggtg tctgataatt cagcgtggag tgcgggcggc attcgttata
      301 atcctgccgc cagtcattaa taattttcct ggcatgaacg atatcgctga accagtgctc
      361 attcaaacat tcatcgcgaa atcgtccgtt aaagctctca ataaatccgt tctgcgttgg
      421 cttgcccggc tggattaagc gcaactcaac accatgctca aaggcccatt gatccagtgc
      481 acggcaagtg aactccggcc cctggtcagt tcttatcgtc gccggatagc ctcgaaacag
      541 tgcaatgctg tccagaatac gcgtgacctg aacgcctgaa atcccaaagg caacagtgac
      601 cgtcaggcat tcctttgtga aatcatcgac gcaggtaaga cacttgatcc tgcgaccggt
      661 ggaaagtgcg tccatgacga aatccatcga ccaggtcaga ttgggcgccg ccggacggag
      721 cagcggcaga cgttctgttg ccagcccttt acgacgtctt ctgcgtttta cgctcaggcc
      781 actgaggtga taaagccggt accgccgaag gactcggcgc aaataaaaaa cgtacatacc
      841 attatacaca gtatgtacgt ttgaaatgaa agccgctgac gttatatgta aagccctgtc
      901 actcactcac acgcgaatag acactgtttt tactatatac tccttcgggg cgctaatgag
      961 gattacttct ttcgaaatgg attaaagcgg ccaagatcat gaggggcgtc tttcggcggc
     1021 atcatcccgg tgcgtttaaa tttataaacc ccgtttagct tcatcgtctg cacgcccaga
     1081 tttaacagtg ccaccagctt cagggtatac agaaacgtga atgccgggac catctgaata
     1141 aaaataaata gcaaaccggc acacatcaca cgcagccagg ctgcatactc catgctcagc
     1201 ggccgcttaa agtaatggat ttccagcaat ccgaccgtct tgttactgat ccacaccgct
     1261 gccagtgaga gtaacaacac gatgcacacc ataatgccca gttccatgat cctcactccc
     1321 cgtcgatttg atattttgag tttctgattt acccgctttg gttttgtttt tcgccttctt
     1381 tttgggtttg atatggtgga acaggaaaca atccaccagc acccaaccat tagcatatcg
     1441 ggccagcagt aatttctggc ccggataatg tgtgtggaaa agcattttca catcaacggt
     1501 attcatgatc ttctgcttgc gcttcagctc ctccccaggc atctcaaatt cctgaataaa
     1561 cggtgatgac aaccgctggc gaagcattcg aattgcacta gcgttttcat cgtgttttgc
     1621 cagcctcccg aatcgcacaa gcgccttatg agttacctga acaggcccca gatgggaatt
     1681 aaccagttca tactgccagt agtatcgcag atccactttt acccagctga tgctgtcttc
     1741 cagtgcggct ttcatgatgg ttcccgacat cgggaccatg gtcagcttgg ccagacgcat
     1801 ctgaatgtaa tcagccaggg gcctaaggga ctcaggtaca ttgctgttca ggagaaggtg
     1861 ctgaagcccc ggtgtcgacg tctgccagct caggttgcgg ccgtcaggca aaaattcaaa
     1921 gttctgatcc gctggcgccg attttgcgtt gttctctttc cgcatcagaa aataatgccc
     1981 acacagtaac tcggcccgta cctggtcctc tatcgggtca tggcgggcct tactgagatc
     2041 aaccgtcaac acaacacggt tgatggcatc ctcatggtat tttttcaccc agacacagac
     2101 acgataaagt ctctcatcaa gtttctggct ggtcgtggaa atccattgca tgcttttctc
     2161 ctcagacctt tctcataatg aaaatgccaa gatctgcacc gggtacaagt tccgtgtagc
     2221 tatggttgct ccttcgacca ttggacagtg cttcatcctg cgctggcacc cagtcctgaa
     2281 gtcttttaat tgttggcttt tgtccgacgc caaaaatggg taatgaggct ttcaccacct
     2341 tgtaatcagt ctcggcatat ttgctacttc cacgctcctt gccttcaggc cggtatgtga
     2401 acaaatgcgc tatcggaacc gggaaggtat tggttcgaca aaggcgatac ccttcgccgt
     2461 ctctcagtac ttttctttcc agtaatgcct tcgctctatc gatgttcgcc accactgtca
     2521 ggtcagttgt cacgggcgat ttgaccgcta ctaaacgacg cttatccagc atcgcgatga
     2581 gatcacttgc gtttgttttt acctggtttc ggtttagccg ccaggcaaaa gtaacttttt
     2641 tgagctgttc ttccacaaac agaattttgc gagtggaatg cgcagtcacc agaccgggaa
     2701 gcttacggtg aacttcctta aaccggatgt gtttctccga attcaccctt ttcattgcag
     2761 aggcaaagtc acttttggca tcatttatat cgcaaataat tccgctcagc tgttcggcac
     2821 cctcaggaac aataatcaac cccgggtatt tggttgtcac tttcgtcgaa ttcctgctga
     2881 gtacatcaat gtcatcgata cagaaatcct gaataacacg gataatttca tccacggagg
     2941 catctgaaat agtactcacc tcgatggggt tcacctcttt caaccgctct tcgtgtcgtg
     3001 tgacggtccc cacagtcatc agctcggcca tcaccggctg atggtcattc agaaaggatc
     3061 tgagcttttc aattcgctcc agaatccggt cacggtaaaa gagtaaatct tcactggcca
     3121 ttcatcactc cggtttcaat aaagcagcgc aacaccgtta cgtcttcaac gacttctgaa
     3181 gagtcgatat gcagccggta gttgtacttt tacagcacct tccgcaattc cccgggattg
     3241 ccggttgtat aaagtggcag aggttcaacc tgctgaccgt catgccgaaa acaacgatta
     3301 gggtcattca aaaggccaaa actgaccggc tggcgttgca gattgatgcg ctgccagtcc
     3361 tgctttacac caaacttcac ccaccaatcc agaccaatca gttcattacg tatgttctgc
     3421 acattgccca aatccgatac tgaaacatcc gggattacac ccttcccttc caccagcaaa
     3481 tgcatcacgc tctaaaaatc aggctgacgg ctcttccttg cgtgcttcag aattgtacga
     3541 atcgaacgca agctgtggcg ggaatgcaaa ttacgacgtt cagccgaact aagccatctc
     3601 tggataagct catttgaata tgacaccggc atttcaccgg ggcctgacct ttcgatttca
     3661 cgcacataca gcaggcagga agtagcggca ctcactactg cgtcagaatt ttcgttcatg
     3721 ttcacgtccg ttaatatcag aaatctgctt atccggtgtc aggaacaaag gctgatagtt
     3781 caggcgccac ggatagtgta ccgtcaaacc atccgttcca atccggttca accggtagcg
     3841 tccattaatt ctgtcaacag accaggtgcc aggaactaca gaacgactcc agcggaaaac
     3901 cggcagatct ccgagctgaa gtgtattcac gagcccttta tccatctcgg tcacgcgtac
     3961 cttcaacaag aagcccagcc agggtaaaga ccagatacac agccaaacgg gctggaggac
     4021 aactttgttt aacaaatgac ctgtcatgag catcgctgat aaaaacataa aactgatgat
     4081 aatccagaac accatccatc ctaaccaggc tcttgtctca cgaagtttcc attcaagaag
     4141 gtaaagtcgc tttttcatcc agagcccccg gaactcagtg aatgactgat gtctgctgca
     4201 tcaaaatagt ggtgcgtggc aggatctcgt ttcgccacaa ctcttgagcc gtcgtccatc
     4261 catgtagaat tccgttgcca cagtgtgaat tgaacgccat gacctcatca gttagctgat
     4321 agaccagcct gtctggcatt tcaatgtact ccggaagcga aagcaggacg ccgtgtatca
     4381 cgtcgccggt aagcctcgac aacgtctctt cgtcgtccag attctttgtg ggcatcgtcg
     4441 aaagcgggat agagaaacaa cagagatcca ttcccaccgc gtcaaaacca acatcaaaac
     4501 cttcgtcatt gagcacaaat cggaagatag cctcaggcgc ctgttctaga cccggcagcg
     4561 agccctccgg cacttctgtg ccggtaaaaa acttatcagt ccagttactg aatggactgg
     4621 tgaacaccca acaaagcaga ggcaacgcaa tatccgtttt aagtactcgt ttcgtgccgg
     4681 acgttcctga agagtgaatt ttgcttttca ttatttacct gtagagtgct ttacttacag
     4741 ctatataaac acattccgga atttccccaa taggttggaa aacactaaaa ttcacagata
     4801 ctggttccct tctgttttag ccggtgctca agatagatac ttgccaggtc gagtggctga
     4861 ttttcttcga gacaggcctg aacaaggtct tcagtggata cggataccag ccctcgggtt
     4921 tcaagaattt ctttcgccgt cgtcaatgct cgtgaggcgc ttcgacttag ctgaattttc
     4981 acctgagaac tgttttcaca cagggcatca gctggtacgg tctgtgaatc tgtattgctc
     5041 ataggggtat cactccatat cagggatcga atgaaaatta actgcaatag acttctctgg
     5101 ttgaccgttt tctacagtgc ttagccaggt tcgagccgga agttgttgct cacaaagaag
     5161 cagatagccc tcgccatttc ggtgcttttg tcccagagca tgcaggagat ccccagaata
     5221 tggacccaga gcagttcggg tataaaacaa cgtcccagaa tgtatcactt tcgacaattt
     5281 gctaagcaaa acggtcttct cgtcttcatt ttcgggtaag acaaggatta tcctgaatcc
     5341 cttggctaca tgtgattgga gatctgccac cagcgtccga aacctgaacg cataaataac
     5401 caatctgaat cgggtcatta acagcgttgt cgcggcaaaa ataacgacaa ctgagccgaa
     5461 tttagaacgg tagaaccacc agttgagggc accggccact atcaaaacga ccagcagcac
     5521 aatgcttccg taaaaaaatg cagccggaaa acgggttctt tttccttcga ggaacgcaga
     5581 tgccagtatt acgcctgtga tgcaatacac aacccagtca aagaacgatt cagtcatcat
     5641 taggtgatag tgtctcgtgt tgttttaaat cggacgcctg ccgttataaa gtcctcagca
     5701 atcgtgtccc atgcagccat caacttcgct aaaattgtca caactctcgg ttgtaacgta
     5761 acgttcccca ctagctaaac ggcgctgtaa atcgatcttt acttcagccg ccggtttgag
     5821 aaacagatat tccagttgtg agtcagttcc tgaagaaagc agtagctcaa tactgaaatc
     5881 aatatgccgg tatgtgaaat ggctattgtc attagagaag accgaattca cagggttatt
     5941 gctcaggatt gtagtggcag gcatagtgac tcctttgttt taaacagtat acaaatctct
     6001 aacgatccat ttttcagata agagacctat tttcgcgaaa ccgaaacaaa agaagtaaaa
     6061 agtaatttgg aggctccagc attcggactg tgtgctgccg gaaacgtcag caaggggcaa
     6121 aacattcgag taaaagaagc agagaaggaa acagctggaa ggatgtaaat taggctaggt
     6181 ttggttgaag aaggtaatgg aaggcttaca tgtaacggtg ccaagagtac agtaccgtaa
     6241 aacatatagg gcagtaagaa cgttgtaaaa ttgtgaaatc aatgcaagtt ttgacgaaag
     6301 caaaaaaaaa ggcacggata accgtgcctt cttagaagta taaaaacctg aaaatatata
     6361 attcaacctt caggactcat gccgcatcaa agattttatc ctctgatgct gggatgaatt
     6421 cacgtaagtt agcgaacacc tcagaaatta attcctgacg gtcatggatt ttaatccgac
     6481 caccagctaa ttctgataca ttcacccctt ggtaaagttc agtaacaact tcaccatctg
     6541 tatcaaggac tctaacatta gcaaccatag gaaagtaaga ttttgaagca tatccttcat
     6601 aaatcccaat aattgattca ttattacggc gtaaaaccac cttgccagac ggcagcgttg
     6661 tttcatccat ataacaatcg gttaattgct cttcgaaatc gtcctcttta agtgaaatat
     6721 gcaaactaag ttcgtcggta atacgacgcg tatatgaata ctcactaaat gtataaccgg
     6781 ccatgtttat atcactgaac gtgcgataaa cctcataaag cccggatgtt atttcatctg
     6841 atacatagtt gataagccca ataaaatgcg tatcccagaa tttttccagt gcaaatcggg
     6901 cgattaaaac ctgaccatca acttccatca gtcttttgta ctcttcacga tcatagccac
     6961 ctggctgggc gctgtccgca gagttactca ggatctcact acagacatcg tcgtattcaa
     7021 agcggctggt cgcgaaatgt ttaccggaaa tatccagata ttgaccggaa accaggtcga
     7081 aaacctcaag gaagtaaacc agtcgcttct tgagtgcatc cgcatccagg ccagccagtt
     7141 gggtatcatt aagagtatcc acataatgcc ccgcagattc tcttaactca ctggctgata
     7201 tactgcatgt aaacgaaact tcgtcgctct ggcaatgaga gacactgata aacatctctg
     7261 tttcatcaaa atcaatacca atgcctgcca gtactgcacg aacgtagttc tggttatcag
     7321 tagaattagt catcttcact tcctcaggat tattaaagga agtgccccac aggggagcga
     7381 cttccccagt gggtggatta aaaatatgta ctactgcgcc attctttcct gcaagttgat
     7441 cagaataggt agcgcggtca tttggtagaa caaagttact ttttggaatg aagaggtaaa
     7501 aaacaagggt taaacaacat cccgtaactc ttcgaccgct tcttggacca acgcaactct
     7561 gtcacaaacc tgcactcggc cggtaggtcc cacggaacag taagtaccgt gcgatgattc
     7621 agacaaaaca ctcccgtcac agccagtgaa tcgaacggtc atcattacag gaacatcctt
     7681 tccggaacta acgccagtat aaagacgaat gattttttca tcatcgcgat acactcccgt
     7741 cgtcccttca gactccttga aaccgatgta aaggtctggc atacaggtcg ggtattcatc
     7801 tgtattcaaa tcaagatgca aagtcaggtc atcttttaca cgacgctgat attgatattg
     7861 acctaaacgg ttaaatgagt tctttacaga tttaattatc tggagagaag tattcaaaca
     7921 cttggagata cttttctcga actctaagat gaaatcacca aaatcagaat ggaaaaattg
     7981 cttaagtgca aaacgggcaa tgagaacttt accctcaaca ctcatgcgat tttgatagat
     8041 ttcctttctt aaaacaggat ctattgaatc atgttcagac tccggtacag accccgccaa
     8101 tattaatgat gcgatatcga caatatctat agtgctgtgc gcgttatgat aaccgcgaat
     8161 tcttatgttc tgaaaaccag acttttcata tactttaaga aagtatttga ttttagtccg
     8221 aagga
//
LOCUS       DAFNBJ010000194         8128 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000194 DAFNBJ010000000
VERSION     DAFNBJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.194"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..339
                     /locus_tag="L2X49_004305"
     CDS             <1..339
                     /locus_tag="L2X49_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799299.1"
                     /translation="SDDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVV
                     SSQRLHDMLNPTKDTNWNSTYIYKSRHEMLSVNLTQETLFSSKSQDKHALFPIFTASW
                     RAHRIMNKGV"
     gene            562..1059
                     /gene="ubiC"
                     /locus_tag="L2X49_004306"
     CDS             562..1059
                     /gene="ubiC"
                     /locus_tag="L2X49_004306"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBN4799300.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1072..1944
                     /gene="ubiA"
                     /locus_tag="L2X49_004307"
     CDS             1072..1944
                     /gene="ubiA"
                     /locus_tag="L2X49_004307"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBN4799301.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2099..4522)
                     /gene="plsB"
                     /locus_tag="L2X49_004308"
     CDS             complement(2099..4522)
                     /gene="plsB"
                     /locus_tag="L2X49_004308"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBN4799302.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4693..5061
                     /gene="dgkA"
                     /locus_tag="L2X49_004309"
     CDS             4693..5061
                     /gene="dgkA"
                     /locus_tag="L2X49_004309"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBN4799303.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5171..5779
                     /gene="lexA"
                     /locus_tag="L2X49_004310"
     CDS             5171..5779
                     /gene="lexA"
                     /locus_tag="L2X49_004310"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBN4799304.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5852..7177
                     /gene="dinF"
                     /locus_tag="L2X49_004311"
     CDS             5852..7177
                     /gene="dinF"
                     /locus_tag="L2X49_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBN4799305.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7293..7502
                     /locus_tag="L2X49_004312"
     CDS             7293..7502
                     /locus_tag="L2X49_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBN4799306.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7544..8059)
                     /gene="zur"
                     /locus_tag="L2X49_004313"
     CDS             complement(7544..8059)
                     /gene="zur"
                     /locus_tag="L2X49_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBN4799307.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1840 a         1995 c         2225 g         2068 t
ORIGIN      
        1 agtgatgatg tttcacctat atttatagaa actgaagaaa agttaagaac ctttaacact
       61 aataaattaa cacgaaacga taatatattt atcctgttct cctctgtcga tgatgggcct
      121 gttatggtgg taagctccca gcgcttacat gatatgttga atcctacaaa agataccaat
      181 tggaattcca cgtatatcta caaatccaga catgagatgt tgtctgttaa tcttactcag
      241 gaaacacttt tcagctccaa atctcaggat aaacatgcgc ttttccccat ttttactgcg
      301 agttggcgag ctcatcgtat aatgaataag ggtgtttaag taaaggaaaa catcaccgtt
      361 cctggcatcc tgggcggtga tgcccctatg gttgccctct ccagcgcggg catcggcaaa
      421 gcgtaaggtt caacagcgtt ttaccacttc atgcgattgt tgcgtttttg ttgcgtatta
      481 gatcacttaa tttgctttac atctcccgta aacacttttc tgcgatacaa tgcctttacg
      541 ttatgtaacg gagagttcgg catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc
      601 tattttaaag agatccctgc cctggaaccg caactgctcg actggctgtt gctggaggat
      661 tccatgacaa aacgttttga acagcaggga aaaacggtaa gcgtgacgat gatccgcgaa
      721 gggtttgtcg agcagaatga aatccccgaa gaactgccgc tgctgccgaa agagtctcgt
      781 tactggttac gtgaaatttt gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc
      841 gtcgttcctg tgtcaacgtt aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg
      901 ccgttaggac gctatctgtt cacatcatcg acattaaccc gggactttat tgagataggc
      961 cgtgatgccg gactgtgggg gcgacgttcc cgcctgcgat taagcggtaa accgctgttg
     1021 ctaacagaac tgtttttacc ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg
     1081 agtctgacgc agaataagct gctggcgttt catcgcttaa tgcgtacgga taagccaatt
     1141 ggcgcgttac tgctgctctg gccaacatta tgggcgttgt gggtggcgac accgggcgtt
     1201 cctcagctct ggatcctggc ggtatttgtc gcgggtgtct ggctgatgcg cgctgctgga
     1261 tgtgtggtga atgattatgc tgaccgaaag tttgatggtc atgttaagcg cacggcgaac
     1321 cgaccacttc ccagcggcgc ggtaacagag aaagaggcgc gcgcgctgtt tgtagtgctg
     1381 gtactgattt cgtttttact ggtactgacg ctgaatacga tgaccattct gttgtcgatt
     1441 gccgctctgg cgttggcgtg ggtgtacccg tttatgaagc ggtataccca tctcccgcaa
     1501 gtggtgctgg gcgcggcgtt tggctggtcg attccaatgg cttttgccgc tgtgagtgag
     1561 tcggtgccat tgagttgctg gttaatgttc ctcgccaata ttctctgggc ggtggcttac
     1621 gacacgcagt atgcgatggt tgaccgcgat gatgatgtga agattggcat taaatccacg
     1681 gcaatcctgt tcggccaata cgataaattg attattggta ttttgcagat tggcgtactg
     1741 gcactgatgg cgatcatcgg tgagttaaat ggcttaggct ggggatatta ctggtcaatt
     1801 gtggtggctg gcgcgctgtt tgtttatcaa caaaaactga ttgccaaccg cgagcgtgaa
     1861 gcctgcttta aagcatttat gaataataac tatgttggtc tggtactgtt tttaggactg
     1921 gcaatgagtt actggcattt ctgatgatgt aaaaaagccg gatgatcatc cggctttctt
     1981 ctgggttgcc ttatgcgcgg cgcttctcag gcctacacaa cacatcgcaa tttattgaat
     2041 ttgcagatta tggaaggccg gataaggcgt tttcgccgca tccggcaatt ctctctgatt
     2101 acccttcgcc ctgcgtcgca ctctcaatcg tcaaacgcac gtctgatgta atcaactccg
     2161 ccagcaactg ataaaccttc atcgtttctg ccggttcggc gtcgccgcta tcgctgatat
     2221 acccttcatc acgcagcgtc agcaccagag aactgaacac cgccttgtcg aagaactccg
     2281 gcgcgttgat gccgtgcagc acggagagac gttgcgcgac ggtgcggctc tctttctcca
     2341 gcgtaccgcg gttgatcgac gggttggcac tcaacaacca gaaggtgatg gcataacgtt
     2401 gcagcgtttc gcgcgcgcct gcggccagca gctgtagggt gcgagaatgc gccgggttga
     2461 tatgcaactc atcatcttgc agggtaatca gcccctgacg ttgcatctca tttgccagcg
     2521 catcaataac gtccggcaac tcgtcgcgat cccagcgcag gaacagctcc gctttcagca
     2581 ttgggtaaag cacattgacg tgctccatca atacgtcgcg ggagatgtgg cgatgctggg
     2641 tgacaattgc cgccatcagc gaaggcagca ccaacatatg cgcaatgttg ttgcgatagt
     2701 aggtcatcag caccgcttgc tcgcgtggca gaatgatgat gtcgccgata gtgtctttct
     2761 cgacttcaaa cttgttcatt tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag
     2821 gaacggtaga gtccgtggag taaggtacgt tgcgcatcag atccaggtag cagttgagtt
     2881 gctcggttaa ctgctcgcgg gtgagtgagc gctgacgtga tgccagaagc gcagtacaac
     2941 acaggttcat ggcgtttgcc gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa
     3001 tattattgac cgtcggcgtt aaccaggccg gacgcaccgc ttcgatggga tcgatagatt
     3061 cacgccagtc tggtacgtgc tggttaaggt aggtcatcaa cggcattggt tcaccgaagt
     3121 tgacgtaacc ctgaccgaga ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc
     3181 tctctttctc tttcgtcgcg ccgcgcagtt ctttggcgta agtacccact tccatgacgt
     3241 gctcataacc gatatagatc ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg
     3301 cctgaatggt catcgacagc gtaccggttt tcggatccag caaacgcccc gtgcgggaac
     3361 gaccgccttc cacgaagtac tcgacggaat aaccacggct gaacagttcg ccgagatact
     3421 cacggaaaac ggtggaataa agtttattgc ctttaaaagt acggcgaata aagaacgccc
     3481 ccagacggcg gaaaatcggc ccggccggcc agaagttcag gttgatcccg gcggcgatat
     3541 gcggcggcac cagcccctgg tggtacagca cgtaagaaag tagcaggtag tccatgtgac
     3601 tgcggtggca aggcacatac accagctcat gaccgtcgtg ggccagctgg cgaacgcgct
     3661 cggcgttatg gacgttgatg ccctgataaa gtcggttcca ggtgaagccc agaatgcggt
     3721 cagtcaggcg gatcatctcg taagagaaat tcgccgcaat ctcttccatc agcgcaatcg
     3781 cgttctgctg cgctttttca tgggagattt ttttgctgcg cgcttcatct tctaccgctt
     3841 tggcaatggc gcgggaggcg agcagcttat taaacagatc ctgacgagca ggcagacgtg
     3901 ggcccacagc agccagacgt tgacgggcaa agtgcatacg cgccacgcgc gccagtttct
     3961 gtgcgatagt tttatccgtg ccgtgttcat ccgccatacg gcgcagcgaa actgacggcg
     4021 agaaacgcac aaaactgtcg cgaccgagcc acagtacggc gaaaaatttc tgtacgccgt
     4081 taagcatacg cagcggcggg tttacttcgc ctttttcacg ccccggcgcg cgaccaaaca
     4141 tcaccgatac tggcaccatc tgcacatcca gatttgggtt gctacggtgc aaatcgagat
     4201 agtcgtggaa cagcttaata gactcttctt tcggcgtgta ataggtgaat acacgcggcc
     4261 cgccgtgaat gaacacatag cgcggcagta gcgtgccgtc gatttccagc ggctctaacg
     4321 ggtcaggcaa gtcatgagcc agacactggg cgcgtaacgt cagcaaatct gctttcgagt
     4381 tgtacggtaa aacgtacata attggacgag aagtatccag ccccagttcc ggggcaggat
     4441 ctgccggaat agacttgctt tttaccagga tgcttaatgg taaattcagt aatttgtagt
     4501 aaattcgtgg ccagccggac ataaacgatg taaagcctct ggttaataat gcaaatgcgc
     4561 ggcaaggata gcagaaagtc atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg
     4621 ttacgcaaac ccgaatcatc ggatttaacg gtacactgat attgacgctc ataatgtaaa
     4681 aaggttcttt caatggccaa taataccact ggattcaccc gaattattaa agctgctggc
     4741 tattcctgga aaggtttacg cgctgcatgg atcaacgaag cggcattccg tcaggaaggc
     4801 gtagcggtat tgttggcggt ggtcatcgcc tgctggctgg atgtggacgc aattacccgc
     4861 gtgctgctta tcagctccgt gatgctggtg atgattgtgg aaatcctcaa tagcgccatc
     4921 gaagcagtgg ttgaccgaat tggctctgaa taccatgagc tttccggacg cgcaaaagat
     4981 atgggatccg ccgcggtgct gattgccatt atcgtcgccg tgattacctg gtgcattctg
     5041 ttatggtcgc attttggata actcttccag atttcgataa atctctggtt tattgtgcag
     5101 tttatggttc caaaatcgcc ttttgctgta tatactcaca gcataactgt atatacaccc
     5161 agggggcgga atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga
     5221 tcacatcagc cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt
     5281 ccgttcccca aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga
     5341 aattgtttcc ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc
     5401 gctggtaggt cgtgtggctg ccggtgaacc gcttctggcg caacagcata ttgaaggtca
     5461 ttatcaggtc gatccttcct tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg
     5521 gatgtcgatg aaagatatcg gcattatgga tggcgacttg ctggcagtgc ataaaactca
     5581 ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg
     5641 cctgaaaaaa cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat
     5701 tgtcgttgac cttcgtcagc agagcttcac cattgaaggg ctggcggttg gggttattcg
     5761 caacggcgac tggctgtaac atatctctga gaccgcgatg ccgccaggcg tcgcggtttg
     5821 tttttcatct ctcttcatca ggcttgtctg catggcattc ctcacttcat ctgataaagc
     5881 actctggcat ctcgccttac ccatgatttt ctccaatatc accgttccgt tgctgggact
     5941 ggtcgatacg gcggtaattg gtcatcttga tagcccggtt tatttgggcg gcgtggcggt
     6001 tggtgcaacg gcgaccagct ttctctttat gctgttgctg tttttacgca tgagcaccac
     6061 cgggctgact gcgcaggctt atggtgccaa aaatcctcag gcattagccc gtgcgctggt
     6121 gcaaccgttg ctgttggcgt taggggctgg ggcgttaatt gcgctgctgc gtacaccgat
     6181 tatcgatctg gcgctgcata ttgttggcgg tagtgaagcg gtcctggaac aggcgcggcg
     6241 ctttcttgaa atccgctggt taagcgctcc ggcgtcgctg gcgaatctgg tattactcgg
     6301 ttggttactc ggcgtgcaat atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat
     6361 cctcaacatt gtgctggatg tctggctggt gatggggctg catatgaacg tgcagggcgc
     6421 ggcgctggcg acggttattg cggaatatgc aacattgctg attggtctgc taatggtgcg
     6481 taaaatcctc aaactacgcg gaatttccgg cgaaatgctg aaaactgcct ggcgaggaaa
     6541 cttccgtcgc ttgctggcgc ttaaccgcga tatcatgctg cgttcgctgt tgttgcaact
     6601 ctgtttcggc gcgatcaccg tacttggtgc gcgactgggg agtgacatta tcgctgttaa
     6661 cgcggttctg atgacgctac tcacctttac cgcctatgcg cttgatggtt ttgcctacgc
     6721 ggttgaagcg cactccggtc aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt
     6781 atggcgggca gcgtgccgcc agtcggggat tgtagcgtta ctgttttcgg tggtttattt
     6841 gctggcaggg gaacacatca ttgcgttgct gacgtcgtta acccagatcc agcagctggc
     6901 tgaccgctat cttatctggc aggtgatttt gccgttggtt ggcgtctggt gttatctgct
     6961 ggacggcatg tttataggcg caacgcgtgc cgccgaaatg cgtaacagta tggcggtggc
     7021 cgccgcaggt tttgcgctga cgctccttac gctgccgtgg ctgggtaatc atggtttgtg
     7081 gctggcatta accgtctttc tggcgttgcg cgggctttct ctggcggcta tctggcggcg
     7141 tcactggcgc aacggtacct ggtttgccgc aacgtgacgg ttaaaaattc tgaataaatt
     7201 atcctaagcc aaattgctga ctacacttaa tctcacgttc agaagaaaag tgaacgtact
     7261 ctcattcaca acctaacgat gaggtcttga ttatgaataa agatgaagcc ggcggtaact
     7321 ggaaacagtt taaaggtaaa gtgaaagagc aatggggcaa actgaccgat gatgatatga
     7381 cgatcattga aggtaaacgt gatcaactgg tcggtaaaat ccaggaacgt tatggttatc
     7441 agaaagatca ggcagaaaaa gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt
     7501 aattaatccc tcctgcccga cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt
     7561 tcacctgcac agagtgatca tgctggcact gttcaggatg acgacacgct tccacttcca
     7621 cgcatgccgc acagagccca tgtgcttcaa tcacattatg ccgcagggca aaccccattt
     7681 ttgccgccag cgtatgcata atgtcttcca cgccttctgc acactcttct ttcaccgcgc
     7741 cgcagcgatc gcaaataaac atggctgacg tatgcgtggg ctggtcgaac agatgacaga
     7801 gcacataact gttggtggat tccaccttat gcacaaaacc ttgctcaagc agaaaatcca
     7861 gcgcgcgata aaccgttggc ggcttggctt gcggttcagc ttcgcgcagc aaatcaagca
     7921 gatcataagc gctgatagcg ccatcctgca gactcatcag gcgcaacact tccaggcgct
     7981 gtggggtcag gcgcacatta cgctgcgcgc agattttttc agcctgcgct aataactcct
     8041 gcgttgtggt cttttccatc gggcacctca aagtgggggt taagaatgtc cttactttac
     8101 catgttccag gaaaaacacc gagaaccc
//
LOCUS       DAFNBJ010000195         8107 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000195 DAFNBJ010000000
VERSION     DAFNBJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.195"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(210..860)
                     /gene="zinT"
                     /locus_tag="L2X49_004314"
     CDS             complement(210..860)
                     /gene="zinT"
                     /locus_tag="L2X49_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBN4799308.1"
                     /translation="MAIRLYKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1117..1752)
                     /gene="msrQ"
                     /locus_tag="L2X49_004315"
     CDS             complement(1117..1752)
                     /gene="msrQ"
                     /locus_tag="L2X49_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBN4799309.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(1753..2757)
                     /gene="msrP"
                     /locus_tag="L2X49_004316"
     CDS             complement(1753..2757)
                     /gene="msrP"
                     /locus_tag="L2X49_004316"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HBN4799310.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2866..3279)
                     /gene="hiuH"
                     /locus_tag="L2X49_004317"
     CDS             complement(2866..3279)
                     /gene="hiuH"
                     /locus_tag="L2X49_004317"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBN4799311.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3412..4083
                     /gene="hprR"
                     /locus_tag="L2X49_004318"
     CDS             3412..4083
                     /gene="hprR"
                     /locus_tag="L2X49_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBN4799312.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4083..5441
                     /gene="hprS"
                     /locus_tag="L2X49_004319"
     CDS             4083..5441
                     /gene="hprS"
                     /locus_tag="L2X49_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBN4799313.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(5549..6400)
                     /gene="hchA"
                     /locus_tag="L2X49_004320"
     CDS             complement(5549..6400)
                     /gene="hchA"
                     /locus_tag="L2X49_004320"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBN4799314.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDIAGRVYKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(6992..8050)
                     /locus_tag="L2X49_004321"
     CDS             complement(6992..8050)
                     /locus_tag="L2X49_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBN4799315.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKMVGERIL
                     TDRENGEKGDNSQDTSYARVGVKGETQINPELTGYGQFELDLEASNRHNPDQTRLAYA
                     GLSYKDFGSFDYGRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTD
                     FFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSVTYTIDGFSFAGAYANSDRIDFQS
                     SDSKGEKAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDF
                     GLRPSIGYSYSKALDVAGYKDSDRLNYIEIGTWYYFNKNMNVYTAYQINLLDKSDYVL
                     AHGLNTDDQLAFGIVYQF"
BASE COUNT         2244 a         1704 c         1865 g         2294 t
ORIGIN      
        1 gcgtgagcac aggtatcttt ctctgttggc cgtattgtgt atggaatccg ttattggtta
       61 actcaatgcg ggttagctta ttaatataca gacacgtcat agtgataagc gtgtctgtag
      121 ctagcttgcg ttcagtggcg tttgtggatg gaccagtggc agttatgata acttactaaa
      181 gcggcatcga ggcgttatca tgagaatact caatgagaca tcatttcctc gaccacttct
      241 tcgctactca actgatatgg ataatacgtt ggccagtttt ccatttcatt taataaagat
      301 tgctgtgaat cattacccat aaaaatgtga aaatgagatg attttcgtgg tgcaattata
      361 tgatcgctaa attgtatata tttaggggct ttgctttcag gatctttaca ttcgaataag
      421 taacgaacgc ctttcttgcc tgatttatag gtgagtattt tgtatccatc gtaatcatat
      481 ttacacgatg ttgtttcatt atttctatgg aattcaacaa tgccgtcctc aatgccaatc
      541 atctcgatat ctgttgcata acctttgtga taataatctt taatttcagc aaatgtttta
      601 gttttatctg catccgcttt cttctgaaag acggggtcaa gtttgccact ttgcagtaaa
      661 ggataaacgg attgccagac tccatcccag tcactgagcg ttcggttttg tacattggca
      721 tcatcaaaaa caccattagc agctttttgt tcgacctctg ttaagggttt gccgtgtgag
      781 tgatgaccat gcgaaaaggc aggagcgcta acaataaaga cacctaaagc aacagccagt
      841 ttgtaaagac gaatcgccaa aacagtttcc tccagagtta atgtatatga tgtgtaatgt
      901 tatattgtaa catatagcaa aatgcaacaa ctcttaggaa acgagagcat ggctttctca
      961 gttctactta tctatcttat tgaaataaaa taaattattt tggtgatttg aggttgatga
     1021 caaacgaaac ataacctgat gcgatctgtg taggccggat agggcgctcg cgccacatcc
     1081 ggcagggata acgcgtactt tgtcggcaat atgaagttat acagataatt tgttgtgaac
     1141 ctgcttgcgt aaccggttaa aaagggaacg caacttctta taccgtaagg ctaaaagcag
     1201 tacagccagc ccagcgtaga tgagcggctg cggtgaaata atcttcacgg accacagata
     1261 atgtattggg gccagaatcg cgacaagata gacgaaatta tgcaactgtt gccagtgctt
     1321 gccgagtttt cgctgcatcg cctgggttga agtaaacgct aaagcaagca aaataaccca
     1381 gctgataatt cctaacgtta aataaggccg ggtaattaac tccttgccta ataacgccag
     1441 attattcacg cccagctcca gcaatgcgta actggttaaa tgcagtgtcg cccaggcaaa
     1501 gcaccataag cctaacaggc ggcgagtgcg tatcaataac ggctgttttg cgtagcgtgc
     1561 cagaggtgtg attaacaagg tcgccagcaa aaatttcagt gcagtgcgac cggtaaaatg
     1621 ctgaatatct ttcaccggat cggcacccag tccaccgtga ttgatcgccc agaccagcca
     1681 gagaaacggc aacaatccgg caagatgcag gctaactttc agccatgtca cctgttttgc
     1741 tgtcagacgc attcagaaat tctcccgcaa atccaggcca cgatacagcg atgccacctg
     1801 gtcggcgtaa ccattaaaca gtagcgttgg ctggcgctga acatcgagga tgccgcctga
     1861 accaataaat cgttcagtag cctgtgacca gcgcgggtga tcaacatgcg gattaacgtt
     1921 ggcgtaaaaa ccgtattcgt caggcgctgc cagattccag gtggttggcg gacgctcgcg
     1981 ggtcagctta atactgacta tcgatttaat ccctttaaag ccatatttcc acggcacaat
     2041 cagtcgtacc ggcgcgccat tttgtggcgg taacgccttg ccataaacac ccacggtcat
     2101 cagtgtgagc ggatgcattg cttcgtcgag acgcaatcct tcgacataag gatatttcag
     2161 cccgccgccg ataaagcggt cctgctggcc tggcatttgt tccggtgcat aaattgtttc
     2221 gaaagcgaca tacttcgcat tgctggtggg ttcggcaagc gccagcaatt tgtgcagcgg
     2281 aaaaccaatc cacggcacca ccatcgacca tgcctccacg cagcgcatac gataaatacg
     2341 ctcttccagc gggaagcgac gggttaaatc atcgtgatcg agggtcaatg gttttgccac
     2401 ttcgccgctg attttcagtg tccatggatc ggttttcagg ctaccagcat tagcggcggg
     2461 atcggcttta tccagcccga attcatagaa gttgttataa ccggagactt tatctactgg
     2521 cgtcagtggc aggttatttt gccaggcggc aggcttgctg aactccagcg gttttccggc
     2581 gggggcgggc gggcgatcgt tccctttaaa ccagctaagc agatcggcat gcgcagcgtg
     2641 aggcaaagaa agtgcagctg cgctgatgcc cagtgctttt aacacctgtc gacgcttcat
     2701 aaagaatacc gactcggccg taacatctga ttcttttaaa aattgattct ttttcataag
     2761 ccatcacact tttttgtcgt tagccagcaa gagccgttta ctgttaatct gtcagggagt
     2821 gtcctcccta acagtagcag agatctgctt ttacatattt atgcattaac tgccacgata
     2881 ggttgaatac ccatattggc taagtaataa aggcacatga taatgctcgt tcactttatt
     2941 aatatgaaat tcaacgggga tctcagggaa gaaactttca agattttgtt tcttgaaata
     3001 gtccccggtt ttaaatacga cacggtaatc gcccgtagtt gcagtttgct cgggccacag
     3061 tgccttaatt cgtccatcct tatctgtttt ggcggtatta agttgtaacc agccgttgtc
     3121 cgctttcttt tcaagagtga ctgtcacgtc ggcagcgggt tttccggttt gctggttcaa
     3181 aatgtgcacg ctaagaatgt tttgttgtgc ggcataaacc aaagaaggta atgaaaatgc
     3241 tgccgttact acggagagta ctaaataacg ctttaacatg tttatatcct tgtcatgtga
     3301 atgagttttg aggatagcga aaaagcggcc agggtaacct tacagcagca ttacaatttt
     3361 gtaatgaacc aggctgtttc tataacatat gatttatggc atattatttt catgaagatt
     3421 ttacttattg aagataatca aaggacccag gagtgggtaa cgcaggggct ttccgaagcg
     3481 ggttatgtca ttgatgccgt ttctgatggc agagatgggc tttatcttgc gctgaaggat
     3541 gattatgcat tgatcattct ggatattatg cttccgggta tggatggctg gcagatctta
     3601 caaacgttaa gaacagcaaa gcaaacccct gttatttgcc ttactgcaag ggattctgtc
     3661 gatgacagag tcagagggct ggacagtgga gcaaatgatt atctggtaaa acctttttca
     3721 ttttctgagt tgctggcaag ggttcgggca caattaaggc aacatcacgc tttgaattca
     3781 acattagaaa tcagtggctt aagaatggac tctgtaagtc aaagtgtgag cagggacaat
     3841 atcagtatta cactgacgcg caaggagttt cagttacttt ggctactggc ctccagagct
     3901 ggcgaaatta tacccagaac ggttattgcg agtgaaattt ggggaatcaa ctttgatagt
     3961 gataccaata cggtggatgt cgccattcgc aggctccgcg caaaagttga tgatcctttt
     4021 cctgaaaagc taatcgccac aatccggggg atgggctatt cattcgtagc ggtaaaaaaa
     4081 taatgaaaag attatctata accgtccgtt taaccttgct ttttatattg ctgctgtctg
     4141 ttgctggcgc tggaattgtc tggactctct ataatggcct ggcaagtgag ttgaaatggc
     4201 gcgatgatac aacactcatt aaccggacag cacagatcaa gcagttgtta attgatgggg
     4261 taaatccaga tacgttacct gtatacttta accggatgat ggatgttagt caggatatct
     4321 tgatcattca tggtgatagc atcaataaaa ttgttaaccg gacaaatgtc agtgatgaca
     4381 tgttaaataa catacctgct agtgagacaa tcagcgcagc tggcatttac agaagcatta
     4441 ttaatgatac tgagattgat gctttacgaa ttaatattga tgaagtttcg ccatcattaa
     4501 cggttactgt ggctaaattg gcttcagcca gacataacat gcttgaacag tataaaatta
     4561 atagcattat aatttgcatt gtcgccattg tactttgctc agtattaagt ccgctgttaa
     4621 tcagaacggg attacgagag atcaaaaagt tgagtggtgt aacggaagcg ctgaattata
     4681 acgatagccg ggagcctgtt gaggttagcg cattaccgag agaactaaaa cctcttgggc
     4741 aggcgttgaa taaaatgcat caagccttag tcaaagattt tgaacgccta agtcaatttg
     4801 ctgacgatct cgctcatgaa cttagaacgc ccattaatgc attactgggt cagaatcagg
     4861 ttacgctcag tcaaaccaga agtatcgctg aatatcaaaa aacaattgcc ggtaacattg
     4921 aagagctgga aaatatttcg cggttaacag agaacatact gtttcttgcc cgggcagata
     4981 aaaacaatgt tttggtgaaa ctggactcgc tttctctcaa taaggaagtc gaaaatttgt
     5041 tggattatct tgaatacctt tcagacgaga aagagatttg ctttaaggtc gagtgcaatc
     5101 agcaaatctt tgcggataaa attttactgc aacgaatgtt atcgaatctt attgttaatg
     5161 ccattagata ttcaccagaa aaatcacgta ttcatataac cagttttctt gataccaacg
     5221 gctatcttaa tattgatgtc gccagtcctg gaacgaaaat tcatgagcct gaaaaactct
     5281 tccgtagatt ttggcgggga gataattcgc gtcattccgt aggtcaggga cttggtcttt
     5341 ctttagtcaa agcgattgcc gaattacatg ggggaagtgc tacgtatcac tatctcaata
     5401 agcataatgt gttccggatt atgttaccgc aaagaaatta acatagggct tcagtacgcc
     5461 ataaatcaac catgaaaaaa cgcagccacg tcgtgagtat taacgtggct gcgattgatt
     5521 atgcgcttac attcatacgt aacagggatt aacccgcata agctgccagc atttcctgcg
     5581 ccgccagttt acccaacgca ttcgctgcga aaggactgtc gccggtgaga actttacggt
     5641 ccttatatac tcgcccggcg atgtcgtcat taataatgtt catgcccatt ttcttcagct
     5701 cttcgccgaa gtaccaggtg agatgacccg gcatatagcc tatctctggc gtttgtttgt
     5761 ctgcggcgtc tgggaatgcg caaatggaat aaccattcag tgggttatcg ccgtggcgaa
     5821 gcgccagaaa agccgccggg ccgtggcaaa gggagataac aaaacggtca tttttgattg
     5881 cccactgtaa agcagcagcc acgtcctggc tttctggtag accaataagt gcgccatgac
     5941 caccaggaac aaagattgct gcatattcgc tatcagcgtt gaggctggcg acaacatccg
     6001 cgagtttctt cggattgcgg aacaacgatt tatgctgctc aaagaatggc attacttttt
     6061 catctttgtg cggcatagcc cagtattcaa acttggtcat cagaccggaa atggtcgcca
     6121 cttcgaattc gaaacctgca gcatggagat gatacaacgg cagcaacgtt tcaatcggat
     6181 ggttaccggt cgagaacagt tttccgttat cggtcggcaa ataacgttcg tccgcggcga
     6241 tcaccagaat tttatgttta ccgcgatacg gttttggata gtccacgcca tcaagatcag
     6301 agacaggact ggtatattgg ctaagcgaat attctgaagg gaagaatgca ttatcttcag
     6361 caatatcgac ctgcggattt ttacttgttt gaacagtcat agtgtattcc ttattgttgc
     6421 ttagttaggg tagtcactat atttgcgact gagctggtcg ctatttttaa ccagtctgga
     6481 aaattgagtc aatatatcga aagcggtagt cggcgggtgt aagtatgtaa tgatgatgct
     6541 gcactggtat tgaatattat tcacgccaaa ttttccttat tgagcaacgt cgtcgcaagt
     6601 ggcctcaatg attttagacc attatcaaag gtacggggcg gcggggagag atttagtgct
     6661 gtgaaatatt gtggtactga tgaatattta tatcgcggat tggtagagaa aaagcttatt
     6721 tcatgcacta tttttgcaaa tatttctata tgggaggcgg ttttgttgca gattaattca
     6781 taaagaaatg catttgggtc ttggctggaa gagtcgctgg tagatgttat tgtgccagca
     6841 ggggcagggt aaggtgctgt atcgatatgc tttggcaatt gcgcgtgccg cagagggaga
     6901 taaaatgcca ataaaaaagc ctgagttcga tgagggacga actcaggcta gtgttaagtt
     6961 ttacagtaac acgccgcggc gcgttggctg attagaactg gtagacgata ccaaatgcga
     7021 gttggtcgtc ggtgttcagt ccatgagcga gaacataatc actcttatca agcaggttga
     7081 tctgataagc ggtatatacg ttcatgttct tattgaagta ataccaggta ccgatttcaa
     7141 tgtagttcag acgatcagaa tctttgtaac ctgctacgtc cagtgcttta gaataggagt
     7201 aaccgatgga tggacgcaga ccgaagtcga actgatattg cagaacagct tcgaagtttt
     7261 gtgttttgtt cacaacatca ccgtcgtcgg aattcatgtt gtgagattca ccgtacatca
     7321 cagcagcata gacgttgttg gcatcatatt ttgttgacag tgcccaaact tctgcttttt
     7381 cacctttact atcactagac tggaaatcaa tacgatcaga gtttgcgtaa gcaccagcaa
     7441 atgagaaacc gtcaatagtg taagttacag ataaaccatg gccatcgcca tttgctttgt
     7501 agttgcctgt accttcattt ttaccctgat attgcagagc aaaatttaga ccttcaacca
     7561 tgccaaagaa atctgtgtta cgataggttg caacaccgtt ggtacggttg gtcatgaaca
     7621 ggtcagtacc agcccatgaa tcaccgcccc actcaacaaa catatcagta aatgcttctg
     7681 catcatacgc gacaccgaca ttacgaccat agtcgaatga accaaaatct ttgtaactca
     7741 aaccagcata agccaaacga gtttggtcag ggttgtgtct gtttgatgct tccaggtcta
     7801 gttcaaactg accgtaacca gtcaattcag ggttgatctg agtttcacct ttcacgccta
     7861 cacgggcata agaggtatct tgagaattat cgcctttttc accattttca cggtcggtca
     7921 gaatgcgctc accaaccatc ttgccgtaca aatccagttt attgccattt ttattataaa
     7981 tttcagccgc atttgctgcg ccagcaacta ataacgccgg gaccagcatt gccagaactt
     8041 ttcttttcat tatgtattcc cttgtgatta taatcttcat gaatatatca ataagtgccg
     8101 ttatcca
//
LOCUS       DAFNBJ010000196         7996 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000196 DAFNBJ010000000
VERSION     DAFNBJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.196"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..810
                     /locus_tag="L2X49_004322"
     CDS             <1..810
                     /locus_tag="L2X49_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HBN4799316.1"
                     /translation="IIAKIDLFIQRITHNLWHARKKNVFLIEQITNLKVWVNKYIDDC
                     TDEDLNDRDFIASVVDRAIFHFAINSICNPGDNKDATPIERCTFDVETKNGLPSTVQL
                     FYEESKDNEPLANIHFQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSAYTDLS
                     ETVYINENNESYLKAQFEKLSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGT
                     LDPDILSKMRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            1173..2210
                     /gene="dusA"
                     /locus_tag="L2X49_004323"
     CDS             1173..2210
                     /gene="dusA"
                     /locus_tag="L2X49_004323"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBN4799317.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2344..2586
                     /gene="pspG"
                     /locus_tag="L2X49_004324"
     CDS             2344..2586
                     /gene="pspG"
                     /locus_tag="L2X49_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBN4799318.1"
                     /translation="MLELLFVFGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2752..3735)
                     /locus_tag="L2X49_004325"
     CDS             complement(2752..3735)
                     /locus_tag="L2X49_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBN4799319.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3818..5233
                     /gene="dnaB"
                     /locus_tag="L2X49_004326"
     CDS             3818..5233
                     /gene="dnaB"
                     /locus_tag="L2X49_004326"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBN4799320.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5286..6365
                     /gene="alr"
                     /locus_tag="L2X49_004327"
     CDS             5286..6365
                     /gene="alr"
                     /locus_tag="L2X49_004327"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBN4799321.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6618..7811
                     /gene="tyrB"
                     /locus_tag="L2X49_004328"
     CDS             6618..7811
                     /gene="tyrB"
                     /locus_tag="L2X49_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBN4799322.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1958 a         1886 c         2067 g         2085 t
ORIGIN      
        1 attattgcaa aaatagattt gtttattcaa agaatcactc ataatttatg gcatgcacgt
       61 aaaaaaaatg tgtttttaat tgagcaaata actaatctca aagtgtgggt caataagtat
      121 attgacgatt gcactgatga ggatttaaac gatcgtgact ttattgcatc agttgttgac
      181 cgggctattt ttcatttcgc gattaatagt atatgtaatc ctggggataa taaagatgcg
      241 actcccattg aacgatgtac ttttgatgta gaaactaaga atggccttcc ctccacggtt
      301 cagctatttt atgaggaatc taaggataat gaacctttag cgaatataca ttttcaagca
      361 ataggttctg gttttttaac gtttgttaat gcctgccagg aacatgatga caacagctta
      421 aaattatttg cttcgctgtt aatttcactt tcatattcta gtgcctacac agatttatca
      481 gaaacagtgt atattaatga aaataatgag agctacctga aagctcagtt tgaaaaatta
      541 tctcaacgtg atatgaagaa gtacctggga gagatgaagc gtctggctga tgggggagaa
      601 atgaattttg atggctatct ggataagatg tcacatctgg tgaatgaagg aacgctcgat
      661 cctgatattt taagcaaaat gcgagatgct gcaccacaat taattagctt cgcgaagtcg
      721 tttgacccaa cctcaaagga agagattaaa atacttacag acacttctaa attaatttat
      781 gatttgttcg gggttaaatc ggagaaataa tatgtgaagt tcttcgatag tatggaaggc
      841 attatataaa aggacccaat atttattggg ttcttttttc tctatcaatg ctattagcag
      901 ggagatatat caccagagtt taatgtgtga ttttttattt atcgtcgaac ctggattgtt
      961 tatcattggc cttaacaaag ttaacggcta ataagattat ttccatcact tcgtgagagc
     1021 ttcatgcctt gagaggatct caattttctt ttgcaatgag acaggcgctt cctgttgtta
     1081 tggtatagta ccccgctatt gagtctcctg aatagtgatg ctgaataaca taaccccatg
     1141 atatatcgat aaaataatct ctacatttga aaatgcacgg taattctgaa atgcaaaaaa
     1201 tcaaccaaac cagcgcaatg cctgaaaaaa ctgacgttca ctggagtggt cggtttagcg
     1261 ttgcaccaat gctcgactgg acggacagac attgccgcta tttcttgcgt ctgctttccc
     1321 gcaatacgtt gctgtatacc gaaatggtga ccacaggggc gattattcac ggtaaaggtg
     1381 attacctggc gtacagtgaa gaagaacatc cggtagcgtt gcaactcggc ggtagcgatc
     1441 cggcggcgct ggcacagtgt gcgaagctgg cagaagcgcg tggatatgat gagatcaacc
     1501 tgaatgtcgg ctgcccgtct gaccgggtgc agaacggcat gtttggtgcg tgtctgatgg
     1561 gtaatgcgca gctggttgcc gactgcgtga aagcgatgcg cgatgtggtg tcgattccag
     1621 tgacggtgaa aacgcgtatt ggcatcgatg accaggacag ctatgaattt ctctgcgatt
     1681 tcatcaacac cgtttccggc aaaggcgagt gtgagatgtt tatcatccac gcacgtaaag
     1741 cctggctttc ggggttaagc ccgaaagaaa accgtgaaat cccgccgctc gattatccgc
     1801 gtgtgtatca actgaagcgt gactttccgc atctgacaat gtcgattaac ggtggtatca
     1861 agtcgctgga agaggccaaa gcacacctgc aacatatgga tggcgtgatg gtcgggcgcg
     1921 aggcgtatca gaatccgggt attctggcgg cggtagaccg ggagatcttt ggttcctcgg
     1981 ataccgatgc cgatccagtg gcggtagtgc gcgccatgta tccgtacatt gagcgtgaac
     2041 tcagccaggg gacgtatctc ggccatatta cccggcatat gttgggcttg ttccagggta
     2101 ttcctggcgc gcggcagtgg cggcgttatt taagtgaaaa tgcccataaa gcgggtgcag
     2161 acattaatgt gctggaacac gcgctcaaac tggtggcgga taagcgttaa cttttcacca
     2221 aaaagtagtc aaattcacca cgccctgcgc accgtcgcgg ggcattttgc tgttaaatca
     2281 atagattatt tttggcatga ttcttgtaat gccagcaaga gatttcatat ttgggagagc
     2341 atcatgctgg aactactttt tgtgtttggc ttttttgtca tgctgatggt caccggcgtt
     2401 tcgttgctgg gcattatcgc cgcgctggtt gtggcgacgg ccattatgtt cctcggcggt
     2461 atgctggcat tgatgattaa gttgctgccg tggttactac tggcgattgc ggtggtgtgg
     2521 gttattaagg cgattaaagc accaaaagtg ccgaaatatc agcgttatga ccgctggcgt
     2581 tactaaggga ttgtgcggat gatcacaacc taaggtttta tccttagaac aaaataggaa
     2641 ttgataatca aatctgtcac tattgcgcct ctaacagatt catcgtgctg taccctacat
     2701 acagccgaac tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat
     2761 cagcaggctg gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc
     2821 cttcagcgga tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa
     2881 agagaacagt tcattactgg cctcggttaa ttcctcccgc gtggtgatat agccttgcag
     2941 ggaagggcgt gtcacataca acgagccttt ttgattgaga atgcctaagt tcacaccggt
     3001 aaccgcacct gatgagttgc caaaactgac cattaagccg cggcgttgca ggcaatccag
     3061 cgaccgttcc caggtgtctc tgcccacgga atcgtacact acgcgcactt tcttaccgcc
     3121 ggtgatctct ttcaatcgct cgaccagatt ctcttcacga tagttaataa cctgccacgc
     3181 gccggctttt agcgcgctct gcgctttttg cgcggttcct acggtgccga taagtttcgc
     3241 gcccagggct tttgcccact ggcaggcaat taagccaacg ccgccagccg ctgcgtggaa
     3301 caggaactgt tcatcgggtt taatttcata ggttttgcgc agcagataat aaaccgttaa
     3361 gcctttcagg aaagatgctg cagcttgttc aaaagaaatt gccgcaggca gaatcgccgc
     3421 tttatccgca ttaatgttat gcacagagct gtaagcgcct aacgccgact gcgcatagac
     3481 tacacgatcg cctgccttaa tatgctttac accactgccg actttactca cgatgcctgc
     3541 cgcttcggta cctaatccgc tgggtagtga tggcggcggg taaaggccgc tgcggatata
     3601 cgtgtcgata aaattgatgc cgatggcttt attttcgacc tggatttcat tctctgccgg
     3661 atcggcagga gtgaactcta cggcttgaag tacttccggg ccaccgtgct tgtgaaattc
     3721 aattcgtgtt gccatgtgtc ctccagaacg tatcgtcagg gtctgcttca tatggtaaag
     3781 tttcgaccca ttctttatct cggtaactcc attcactatg gcaggaaata aacccttcaa
     3841 caaacagcag gctgaacccc gcgaacgcga tccacaagtt gccgggctga aagtgcctcc
     3901 gcactcgatc gaagcggagc agtcggtgtt gggcggttta atgctggata acgaacgctg
     3961 ggacgatgta gccgagcgtg tggtggcaga cgatttttac acccgcccac accgccatat
     4021 ctttactgaa atggcgcgtt tgcaggaaag cggtagtcct atcgatctga ttacccttgc
     4081 ggaatcgctg gaacgccagg ggcaactcga tagcgtcggt ggttttgctt atctggcaga
     4141 gctgtcaaaa aatacgccaa gtgcggcgaa catcagtgcc tatgcggaca tcgtgcgtga
     4201 acgtgccgtt gtccgtgaga tgatttcggt tgcgaatgag attgctgaag ccggttttga
     4261 tccgcagggg cgcaccagcg aagatctgct ggaccttgct gaatcccgcg tctttaaaat
     4321 tgctgaaagt cgtgcaaaca aagacgaagg gccgaagaac atcgccgatg tgctcgacgc
     4381 aaccgtggcg cgtattgagc agttgtttca gcagccacac gatggcgtta ccggagtaaa
     4441 caccggttat gacgatctca acaaaaaaac cgctggcttg cagccgtcgg atttgatcat
     4501 cgtcgccgcg cgtccgtcga tgggtaaaac aacatttgcg atgaacctcg tcgaaaacgc
     4561 ggcgatgttg caggataaac cagtacttat cttctcgctg gagatgcctt cagaacagat
     4621 tatgatgcgt tctctggcgt cgctgtcgcg cgttgaccag actaaaatcc gtaccgggca
     4681 gctcgatgat gaagactggg cgcgcatttc cggcaccatg ggtattttgc tcgaaaaacg
     4741 caatatctat atcgatgact cctccggcct gacgccaacg gaagtgcgtt cccgcgcacg
     4801 ccgtattgcc cgtgaacacg gcggcatcgg gcttatcatg atcgactacc tgcaactgat
     4861 gcgcgtaccg gcgctttccg ataaccgtac gctggaaatt gcagaaatct ctcgctcgct
     4921 gaaagcactg gcgaaagaac tgaacgtgcc ggtggtggcg ctgtcccagt tgaaccgttc
     4981 tctggaacaa cgtgccgaca aacgcccggt caactccgac ctgcgtgaat ctggctctat
     5041 cgagcaggat gcggacttga tcatgtttat ctatcgtgat gaggtgtatc acgaaaacag
     5101 tgatttaaaa ggcatcgcgg aaattattat cggtaaacaa cgtaacggcc caatcgggac
     5161 ggtacgcctg acctttaacg gtcaatggtc gcgcttcgac aactatgcgg ggccgcagta
     5221 cgacgacgaa taataattat tttatgaatt aggtaattaa agcaaacact tatcaaggaa
     5281 cacaaatgca agcggcaact gttgtgatta accgccgcgc tctgcgacac aacctgcaac
     5341 gtcttcgtga actggcccct gccagtaaaa tggttgcggt ggtgaaagcg aacgcttatg
     5401 gtcacggtct tcttgagacc gcgcgaacgc tccccgatgc tgacgccttt ggcgtagccc
     5461 gtctcgaaga agctctgcga ctgcgtgcgg ggggaatcac caaacctgta ctgttactcg
     5521 aaggcttttt tgatgccaga gatctgccga cgatttctgc gcaacatttt cataccgccg
     5581 tgcataacga agaacagctg gctgcgctgg aagaggctag cctggacgag ccggttaccg
     5641 tctggatgaa actcgatacc ggtatgcacc gtctgggcgt aaggccggaa caggctgagg
     5701 cgttttatca tcgcctgacc cagtgcaaaa acgttcgtca gccggtgaat atcgtcagcc
     5761 attttgcgcg cgcggatgaa ccaaaatgtg gcgcaaccga gaaacaactc gctatcttta
     5821 ataccttttg cgaaggcaaa cctggtcaac gttccattgc cgcatcgggt ggcattctgc
     5881 tgtggccaca gtcgcatttt gactgggtgc gcccgggcat cattctttat ggcgtctcgc
     5941 cgctggaaga tcgctccacc ggtgccgatt ttggctgtca gccagtgatg tcactaacct
     6001 ccagcctgat tgccgtgcgt gagcataaag ccggagaacc tgttggttat ggtggaacct
     6061 gggtaagcga acgtgatacc cgtcttggcg tagtcgcgat gggctatggc gatggttatc
     6121 cgcgcgccgc gccgtccggt acgccagtgc tggtgaacgg tcgcgaagta ccgattgtcg
     6181 ggcgcgtggc gatggatatg atctgcgtag acttaggtcc acaagcgcag gacaaagccg
     6241 gggatccggt cattttatgg ggcgaaggtt tgcccgtaga acgtatcgct gaaatgacga
     6301 aagtaagcgc ttacgaactt attacgcgcc tgacttcaag ggtcgcgatg aaatacgtgg
     6361 attaatcgtt ctgtaatatt tgattgtctg tgccggatgc ggcgtgaatg ccttatccgg
     6421 ccaataaaat cctaaaaatt caataagttg atgttctttc atgctcttat aaaggtcgtg
     6481 cctctggcgg atgtacgttt gtcatgagtc tcactctgtt gctaattgcc gttcgctcct
     6541 gaacatccac tcgatcttcg ccttcttccg gtttattgtg ttttaaccac ctgcccgtaa
     6601 acctggagaa ccatcgcgtg tttcaaaaag ttgacgccta cgctggcgac ccgattctta
     6661 cgcttatgga gcgttttaaa gaagaccctc gcagcgacaa agtgaattta agtatcggtc
     6721 tgtactacaa cgaagacgga attattccac aactgcaagc cgtggcggag gcggaagcgc
     6781 gcctgaatgc gcagcctcat ggcgcttcgc tttatttacc gatggaaggg cttaacagct
     6841 atcgccatgc cattgcgccg ctgctgtttg gtgcggacca tccagtactg caacaacagc
     6901 gcgtagcaac cattcaaacc cttggcggct caggggcatt gaaagtgggc gcggatttcc
     6961 tgaaacgcta cttcccggaa tcaggcgtct gggtcagcga tcctacctgg gaaaaccacg
     7021 tagcaatatt cgccggggct ggattcgaag tgagtactta cccctggtat gacgaagcga
     7081 ctaacggcgt gcgctttaat gacctgttgg cgacgctgaa aacattacct gcccgcagta
     7141 ttgtgttgct gcatccatgt tgccacaacc caacgggtgc cgatctcact aatgatcagt
     7201 gggatgcggt gattgaaatt ctcaaagccc gcgagcttat tccattcctc gatattgcct
     7261 atcaaggatt tggtgccggt atggaagagg atgcctacgc tattcgcgcc attgccagcg
     7321 ctggattacc cgctctggtg agcaattcgt tctcgaaaat tttctccctt tacggcgagc
     7381 gcgtcggcgg actttctgtt atgtgtgaag atgccgaagc cgctggccgc gtactggggc
     7441 aattgaaagc aacagttcgc cgcaactact ccagcccgcc gaattttggt gcgcaggtgg
     7501 tggctgcagt gctgaatgac gaggcattga aagccagctg gctggcggaa gtagaagaga
     7561 tgcgtactcg cattctggca atgcgtcagg aactggtgaa ggtattaagc acagagatgc
     7621 cagaacgcaa tttcgattat ctgcttaatc agcgcggcat gttcagttat accggtttaa
     7681 gtgccgctca ggttgaccga ctacgtgaag aatttggtgt ctatctcatc gccagcggtc
     7741 gcatgtgtgt cgccgggtta aatacggcaa atgtacaacg tgtggcaaag gcgtttgctg
     7801 cggtgatgta atgcaggaaa gcaggctgga gctacccagc ctgcagtgaa attaaactgt
     7861 cgtcgctttc actctttctt tatagatgat ttttttgatg ccatcgttct acgtgagaga
     7921 taataaacgt tattagttct tttattgtta agcttatcct aattatctgg aattccttat
     7981 cctgtttttt tgggtg
//
LOCUS       DAFNBJ010000197         7872 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000197 DAFNBJ010000000
VERSION     DAFNBJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.197"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            244..894
                     /gene="csgD"
                     /locus_tag="L2X49_004329"
     CDS             244..894
                     /gene="csgD"
                     /locus_tag="L2X49_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBN4799323.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQRVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            899..1288
                     /gene="csgE"
                     /locus_tag="L2X49_004330"
     CDS             899..1288
                     /gene="csgE"
                     /locus_tag="L2X49_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBN4799324.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1313..1729
                     /gene="csgF"
                     /locus_tag="L2X49_004331"
     CDS             1313..1729
                     /gene="csgF"
                     /locus_tag="L2X49_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBN4799325.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1756..2589
                     /gene="csgG"
                     /locus_tag="L2X49_004332"
     CDS             1756..2589
                     /gene="csgG"
                     /locus_tag="L2X49_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HBN4799326.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYLRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2653..3144)
                     /locus_tag="L2X49_004333"
     CDS             complement(2653..3144)
                     /locus_tag="L2X49_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBN4799327.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3246..3800)
                     /gene="ycdY"
                     /locus_tag="L2X49_004334"
     CDS             complement(3246..3800)
                     /gene="ycdY"
                     /locus_tag="L2X49_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBN4799328.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3824..4561)
                     /gene="ycdX"
                     /locus_tag="L2X49_004335"
     CDS             complement(3824..4561)
                     /gene="ycdX"
                     /locus_tag="L2X49_004335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBN4799329.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4616..5554)
                     /gene="ghrA"
                     /locus_tag="L2X49_004336"
     CDS             complement(4616..5554)
                     /gene="ghrA"
                     /locus_tag="L2X49_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HBN4799330.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGACLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGEKVCGQVDRARGY"
     gene            5788..5875
                     /locus_tag="L2X49_004337"
     tRNA            5788..5875
                     /locus_tag="L2X49_004337"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5822..5824,aa:Ser,seq:gga)
     gene            5920..6056
                     /locus_tag="L2X49_004338"
     ncRNA           5920..6056
                     /ncRNA_class="other"
                     /locus_tag="L2X49_004338"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(6249..7235)
                     /locus_tag="L2X49_004339"
     CDS             complement(6249..7235)
                     /locus_tag="L2X49_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799331.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRCLWHNDFMMACDMYFRWGHFNK"
     gene            complement(7232..7759)
                     /locus_tag="L2X49_004340"
                     /pseudo
     CDS             complement(7232..7759)
                     /locus_tag="L2X49_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         2237 a         1930 c         1750 g         1955 t
ORIGIN      
        1 taataaaacc ttaagattaa cattttaata taacgagtta catttagtta catgtttaac
       61 acttgattta agatttgtaa tggctagatt gaaaccagat gtaatccatt agttttatat
      121 tttacccatt tagggctgat ttattactac acacagcagt gcaacatctg tcagtacttc
      181 tggtgcttct attttagagg cagctgtcag gtgtgcgatc aataaaaaaa gcggggtttc
      241 atcatgttta atgaagtcca tagtattcat ggtcatacat tattgttgat cactaaaccg
      301 tctttgcaag cgacagctct cttgcagcac cttaaacaat cgctggcaat tacaggaaaa
      361 ttacataata ttcaacgttc tctggacgat atatcttcag gctctattat tcttctggat
      421 atgatggaag cagataaaaa gcttatccat tattggcagg atactttgag caggaaaaac
      481 aacaatatca aaatattgtt gctaaatacg cctgaagatt acccgtaccg cgacattgaa
      541 aactggcctc atatcaacgg cgttttttat gccatggagg atcaagaacg tgttgtcaat
      601 gggttgcaac gcgtcctgcg cggcgaatgc tactttacgc aaaagcttgc cagttacctg
      661 attacacatt ctggtaacta tcgttataac agcacggaat cagccctcct tactcatcgg
      721 gaaaaagaga tcctgaataa gctgcgtatc ggcgcgtcta ataacgagat cgctcgttcg
      781 ttgttcatca gcgaaaatac ggttaaaacg catctttata atcttttcaa gaagatagcc
      841 gtaaaaaacc ggacacaagc ggtttcctgg gcaaacgata acctcaggcg ataaagccat
      901 gaaacgttat ttacgctgga ttgtggcggc agaatttctg ttcgccgcag ggaatcttca
      961 cgccgttgag gtagaagtcc cgggattgtt aactgaccat actgtttcat ctattggcca
     1021 tgatttttac cgagccttta gtgataaatg ggaaagtgac tatacgggta acttaacgat
     1081 taatgaaagg cccagtgcac gatggggaag ctggatcact ataacggtca atcaggacgt
     1141 tattttccag acttttttat ttccgttgaa aagagacttc gagaaaactg tcgtctttgc
     1201 actgattcaa actgaagaag cactaaatcg tcgccagata aatcaggcgt tattaagtac
     1261 gggcgatttg gcgcatgatg aattctaaat aaaaaattgt tcggaggctg caatgcgtgt
     1321 caaacatgca gtagttctac tcatgcttat ttcgccatta agttgggctg gaaccatgac
     1381 tttccagttc cgtaatccaa actttggtgg taacccaaat aatggcgctt ttttattaaa
     1441 tagcgctcag gcccaaaact cttataaaga tccgagctat aacgatgact ttggtattga
     1501 aacaccctca gcgttagata actttactca ggccatccag tcacaaattt taggtgggct
     1561 actgtcgaat attaataccg gtaaaccggg ccgcatggtg accaacgatt atattgttga
     1621 tattgctaac cgcgatggtc aattgcagtt gaacgtgaca gatcgtaaaa ccggacaaac
     1681 ctcgaccatc caggtttcgg gtttacaaaa taactcaacc gatttttaag ccccagcttc
     1741 ataaggaaaa taatcatgca gcgcttattt cttttggttg ccgtcatgtt actgagcgga
     1801 tgcttaaccg ccccgcctaa agaagccgcc agaccgacat taatgcctcg agctcagagc
     1861 tacaaagatt tgacccatct gccagcgccg acgggtaaga tctttgtttc ggtatacaac
     1921 attcaggacg aaaccgggca atttaaaccc tacccggcaa gtaacttctc cactgctgtt
     1981 ccgcaaagcg ccacggcaat gctggtcacg gcattgaaag attctcgctg gtttataccg
     2041 ctggagcgcc agggcttaca aaacctgctt aacgagcgca agattattcg tgcggcacaa
     2101 gaaaacggca cggttgccat taataaccga atcccgctgc aatctttaac ggcggcaaat
     2161 attatggttg aaggttcgat tatcggttat gaaagcaacg ttaaatcggg cggtgttggg
     2221 gcaagatatt ttgggatcgg tgcagacacg caataccagc tcgatcagat tgccgtgaac
     2281 ctgcgcgtcg tcaatgtgag taccggtgag atcctctcct cggtgaacac cagtaagacg
     2341 atactttcct atgaagttca ggccggggtt ttccgcttta ttgactacct gcgcttgctg
     2401 gaaggggaag tgggttacac ctcaaacgaa cccgttatgc tgtgtctgat gtcggctatc
     2461 gaaacagggg tcattttcct gattaatgat ggtatcgacc gtggtctgtg ggatttgcag
     2521 aacaaagcag aacggcagaa cgacattctg gtgaaatacc gccatatgtc ggttccaccg
     2581 gaatcctgac agataaaaaa gccggaatta tcatccggca ttttactgtt taagcgaaag
     2641 cgtgagtgcc cctcacgctt tatttttgat ttcctgctca cggtgagccg tctttgcact
     2701 acgcgccgcc agccacaggc cgctgttttt cattgcgtaa ccaaatacca gcccaagcgc
     2761 cagcgaaggt aacaccagtt tccagtcacc ctgcccggca aacgtcgcgc atgcgcctat
     2821 aaacgtcccc ggaacaaatg aaagcagcag ctgtttggcc tgaatacaca tcagaaacgc
     2881 cacaataccg gttatgacat aaccgagaat ttccagatgt ggtgccagtg cactaccgta
     2941 aataatgacc atcgcccaca ccacgccact aagcagggtt gcagcggaga tcgccagccc
     3001 tttcagccca ccttgcgggc aggcaaagta ggccgtacag cccaggaagc ccgcccagct
     3061 tagtaagccc agggaaacag ccacccatcc ccagataccg gagagaatgc ctgttgtgat
     3121 tgcaatagag agaagtatgt tcatgccgcg catgatagca gaaaacgcgg ccatgaatga
     3181 gatcgagcac acattttatg caacaacaaa ttatctgttg cagttatgag tgacgatgat
     3241 cacatttact cttcagaatc ttcttccagc tcgtcccaca ttgcactaat ggcatcacgg
     3301 gttagcggtg ccatggtgcg ccagaaaggc gtggttgcat gggcctccac tttgccgagg
     3361 aacgcaccac accagggtaa cagatactca ctgaacagtg tttccagtgc ttcgctctca
     3421 tcttccgttg actgatcttc cagccaggaa gccgcaagca gcaatgtgcc gatgtgatcg
     3481 gctggcgtat ccgccagtgg catccctcgt tcggaaagaa aagcgcgcac ttccgcttcc
     3541 gttgcgtcct caacccatgc gctacgatat ggcggcacgg cacattcatc gccgataaac
     3601 aacgcattgt aatcggcaga gacttgcgcc atatcacagc ttttctgtaa acgcgtcagt
     3661 aactcatcct gctccagtgg ccagttcgca gccagtttcc cctcacgaat cagggtaaac
     3721 agcggcacca gtaaaggatc ttgcggttgg cggtaataga gcgaacccag cacacgacag
     3781 aggatagaaa actcgttcat taatatattt ccattactag taattaaagg tctgcaaatt
     3841 ccgcaatcgg tgccataccg cgagattcaa ggaagttcag taagcggcgc ggagaaacat
     3901 tcaaaatgcg ctctggcgga aaatctaccg cgtcgaggat tttaagacac tcttcaaatt
     3961 cccccatggt aaacgcggtg tgagaatccg agcctaatgc cacccaacct ccagcatcac
     4021 gtaccgctgc ggctaccgca cgacagttgt cttcactgcc cttacgtgag tgtaaaaatg
     4081 aagaattatt gatttccagc gccacctgat gtttcgcggc tgcttcagca acggctttca
     4141 catctatttc atatttggga tttccgggat ggcttattat atgcacattg ccgctggcga
     4201 tagtggcgat catcgcttgt gtattggtcg ctttgtcatg tggtgcaaaa accggctcat
     4261 gaaaaccggc aataatcaga tccagcgagt caaacatttt accgctgcaa tcaatttcac
     4321 catcaacatt tttaatgtta gcttcgatgc cgcgcaggat ccctacccca tcaaccactc
     4381 gcggccagat acgcatgtta atgaagtgcc agtgatgcgg agcatcttcc atatccgggc
     4441 catgatcggt gatcgcaaaa agtttaatgc ccttttgttt ggcctgggca atgtaatcac
     4501 tcaatgtgct atatgcatgt gtgctggcaa cggtatgcat atgaaggtcg acgggataca
     4561 taactctctc ctgtattcaa ttcccgccaa ggatagcagg aatcctgatg ctttattagt
     4621 agccgcgtgc gcggtcgact tgcccgcaga ccttctcccc tttttcgagc tgggcaatag
     4681 tgcgagaaat gtactccaca gcttcagcgg gacgggtaat cgcggcgaca tgtggtgtta
     4741 tcgtcacgcg tggatgttgc cagagcggac tttcaggcgg taagggttca cgattaaaaa
     4801 catccaagca tgcgccttta actttcccgc tatccagcgc cgcgagcagg tcatcttcca
     4861 caacatgaac accacgcgcc aggttgagga gatacgcgcc atccggtaat ttttcgagta
     4921 attgttgatt aataatgccg acggtttcag gggtattcgg taacaaatta atcaataccc
     4981 gacattggct cagaaatgca gacagttctt cccgtccggc aaagctttgc acgccaggcc
     5041 acgatttacg ggttcgactc cagcaacgca gcggaaagcg ccaggtttgc agactctgag
     5101 caactttact gcccagtacg cctgcgccca aaatgccgat ggtaaaatct tcccgatgat
     5161 attccggcag cggttgccaa tgcgaactat tttgctggat gcgataatcg tcaaaacgtc
     5221 gaaaccaatg cagcacctga ctgacagcat attcctgcat ttgctcgccc ataccggtat
     5281 cttccaggcg aaaaagtgga acagaagggt tcagcatttc agggtgtgcc tgtagcttgc
     5341 tcaaaataga atcaacaccg gccccgagtg cgaacaccgc tttaagatcg cgccctgcca
     5401 gcatttcaac aggaggatgc cagactaaag cataatcagc agagtcatta tctccgcttt
     5461 tccacgctct gactcttgcc tgaggaatag ctttgcgcag tgcctcaatc caccattggg
     5521 tatcgaacgt tgggtgataa aagatgatat ccatactgac tcccgaaaag cgttgtgcga
     5581 aatttattcg cacttatcgt tatgatctac aaaggccacc agcataacaa atccgtggtc
     5641 ggtggcaaaa aaagcagatt tcgcttatta aaaccacaca ttgattgaag tttgaataaa
     5701 cgcgcgattt tttcaaaaag tttgttgacc tcaggtcatg atttccctaa attagcgccc
     5761 gttccagcaa gacaggaacg acaatttggt gaggtgtccg agtggctgaa ggagcacgcc
     5821 tggaaagtgt gtatacggca acgtatcggg ggttcgaatc cccccctcac cgccatattt
     5881 aaagaagagc tcgtacgaaa gtacgggctt ttttttcgta tattgcacac accgggggga
     5941 tgagaagccc cgaccggggt tcgacaactg gcgacagcca gttggacaga ccgtgaacgc
     6001 agtgagcggg ctgcccgcag ggcgagcgaa gcgagtcaat ccccccctca ccgccatatt
     6061 taaagaagag cccgtacgaa agtacgggct tttttttcgt atattgcaca caccgggtgc
     6121 ccccgcccac gtctgttagg gcgagggaaa attgtgcaca tcaactgtct ggttactcag
     6181 tcaacaatca aaatgcgcaa gatagtagag aacatatatg cgttatctgg tcacttcctg
     6241 taacttcctc acttattaaa atgcccccaa cggaaataca tgtcacatgc catcataaaa
     6301 tcgttatgcc agaggcatcg acaatggaaa aaatagtaaa ttaagcacgg tacatagcag
     6361 agtaaaaaat aggcttcaaa ccaaatccgc cattccacaa gcgttgccaa taccgacacc
     6421 agcgtaatcc acatcatttc aataaccagc aatatgaata accgaagcca aagcggcttg
     6481 gcaaaaaagg tcgacaacgc attttcactg gagaaaaaca gatcgatttc ctgcaaaaaa
     6541 agcctgccca gaaaagcgta ccgcttggag aagcgcagaa aaaacagatt aatcgccaac
     6601 actacgccac aaagaatgag caatgcaatt atcgatttaa acgttaacca ctgcccctct
     6661 gtcacaaaag aatcactgtt acgtaacggg ctgatagtaa gcaggttaac aaccgttgag
     6721 atgcacaccg aaagcagcac atagcgagtc agacttttat attttacttt tttcgctact
     6781 gaaatagcga cctgcttgac ataactggtg cggatcatcc gaaacagcag aaagatgacc
     6841 aacggcccaa gtaacgaaat caggaacagc gcatataacg agggtagaag ctggtatagc
     6901 ccccaactca ttgcactcaa catgattaac gacagaaatc ctgccaccag aggttgtgta
     6961 agaaataatt ctattttgtg gttctgcttt ttcttaaacc acgaaaaatc gatattcttc
     7021 gtgacgcatt tataagccca gtacgccttg agatcaaaaa taaaattata cagcaccagg
     7081 gccagaatcc cactaacgac accaatatcc tctgatggaa aagtaaatcc tgcatttttc
     7141 caataaaagc ctatcagaat aagtccaata gcataaatgt atatcagatt tcgtttatcc
     7201 tgaaagcgaa taaaagttaa ataatcagga atcatttgaa tacctttgca tcactaaatg
     7261 ctttacgaca atgcataact cgttgctgat ccacgccaac aaaatcattt aacatattcc
     7321 ccaaataggg cttattactt atttcaagga aagctaccgg ggttggcata ttcagaaatg
     7381 tatcgagaga ttcagtccat ttcaccgtat gggttaagtg taatgagaga ttttctttga
     7441 taattgacgg agcgacctcg ctttttgctg ttacgttcat cagaacctga tgctctggcg
     7501 aagcgatatc cagtccggca agataatcgc gcattgcctg aacgccgtct tccatcaaac
     7561 gcgtgtgcca ggcaccgctc acaccgagtt taaccggttc gtatccagcg gccatcagca
     7621 gcgtggcaaa ttcattcaac gaggcctgcg ttcccccaat aacctgctgg cgcggcgtgt
     7681 tatcacagct aatatccagc gcaatgcctg attccgtaat catcgtctgc agttgttcgc
     7741 ggttgatgcc tttaaccgcc tgatcttacc cagcaatagt ggacacgcgg ctaagtgagt
     7801 aaactctcag tcagaggtga ctcacatgac aaaaacagta tcaaccagta aaaaaccccg
     7861 taaacagcat tc
//
LOCUS       DAFNBJ010000198         7827 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000198 DAFNBJ010000000
VERSION     DAFNBJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.198"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(52..128)
                     /locus_tag="L2X49_004341"
     tRNA            complement(52..128)
                     /locus_tag="L2X49_004341"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:Arg,seq:acg)
     gene            complement(192..268)
                     /locus_tag="L2X49_004342"
     tRNA            complement(192..268)
                     /locus_tag="L2X49_004342"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(232..234),aa:Arg,seq:acg)
     gene            complement(466..542)
                     /locus_tag="L2X49_004343"
     tRNA            complement(466..542)
                     /locus_tag="L2X49_004343"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(506..508),aa:Arg,seq:acg)
     gene            complement(546..638)
                     /locus_tag="L2X49_004344"
     tRNA            complement(546..638)
                     /locus_tag="L2X49_004344"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(602..604),aa:Ser,seq:gct)
     gene            complement(954..1139)
                     /gene="csrA"
                     /locus_tag="L2X49_004345"
     CDS             complement(954..1139)
                     /gene="csrA"
                     /locus_tag="L2X49_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBN4799332.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1374..4004)
                     /gene="alaS"
                     /locus_tag="L2X49_004346"
     CDS             complement(1374..4004)
                     /gene="alaS"
                     /locus_tag="L2X49_004346"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBN4799333.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4132..4632)
                     /gene="recX"
                     /locus_tag="L2X49_004347"
     CDS             complement(4132..4632)
                     /gene="recX"
                     /locus_tag="L2X49_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBN4799334.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4875..5936)
                     /gene="recA"
                     /locus_tag="L2X49_004348"
     CDS             complement(4875..5936)
                     /gene="recA"
                     /locus_tag="L2X49_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBN4799335.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(6016..6513)
                     /gene="pncC"
                     /locus_tag="L2X49_004349"
     CDS             complement(6016..6513)
                     /gene="pncC"
                     /locus_tag="L2X49_004349"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBN4799336.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6658..7743)
                     /gene="mltB"
                     /locus_tag="L2X49_004350"
     CDS             complement(6658..7743)
                     /gene="mltB"
                     /locus_tag="L2X49_004350"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBN4799337.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1823 a         2192 c         1893 g         1919 t
ORIGIN      
        1 ttaattccgc tacctcttcg ataccttcat tgctgaagat ttcggagaat atggtgcatc
       61 cgggaggatt cgaacctccg accgctcggt tcgtagccga gtactctatc cagctgagct
      121 acggatgcat cgggaaaatt tattttactg ctgatattga taccgctact aaagccatat
      181 caagtaagag atggtgcatc cgggaggatt cgaacctccg accgctcggt tcgtagccga
      241 gtactctatc cagctgagct acggatgcat cgggaaactt actttactgc agattttttg
      301 ataccgctac taaagccgta tcaagtaaga gatggtgcat ccgggaggat tactcgcctt
      361 gcggctcgcc cttcgggccg ttgctaaagc aacgttatcc tccctggtgc ctgcggttat
      421 accgccacct cttccatacc ttcatcgctg aaagtacgta gaatatggtg catccgggag
      481 gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat
      541 gcaaatggcg gtgaggcggg gattcgaacc ccggatgcag cttttgaccg catactccct
      601 tagcagggga gcgccttcag cctctcggcc acctcaccac acgcctctta cgagtgcttc
      661 gaagaacttg tttatcgctc atcgtcgctg cgtggcgcac atattacttt ctgagactta
      721 taagtcaaac aatttttcct acacttttat cgtttgcaca cttcacgttc aattagtctg
      781 taaaaacgtc aaaaagagtg ttttatcaac agaaaaatgg aggtctgaca gatagtagta
      841 atgcaaaaaa atggagactt aagttgaatg aacgggagta aagcgaaaag actatagagt
      901 gaaggagaaa ttttgagggt gcgtctcacc gataaagatg agacgcggaa agattagtaa
      961 ctggactgct gggatttttc agcctggata cgctggtaga tctcttcacg gtgaacagaa
     1021 acttccttcg gggcatttac gccaatacgt acctggttgc cctttacccc taaaactgtc
     1081 acggtgacct catccccaat catgagggtc tcaccaactc gacgagtcag aatcagcatt
     1141 ctttgctcct tgaaagatta aaagagtcgg gtctctctgt atcccggcat tatccatcat
     1201 ataacgccaa aaagtaagca atgacaaaca cattacatgt aagcagtcat ggcattacat
     1261 tctgttaaac ctaagtttag ccgatataca caacttcaac ctgactttat cgttgtcgat
     1321 agcgttaatg cgaatgccgt gaagcgagtc cacggcattg cctgacgctt atattattgc
     1381 aatttcgcgc tgacccagcc tttcacactg gctaacgctg caggtaaggc cgcagcatcc
     1441 gtaccaccgg cttgcgccat gtcaggacgt ccaccaccct tgccgcccac ctgctgagcg
     1501 accataccaa tcagttcccc tgctttcaca cgatctgtga cgtccttaga tacgcctgca
     1561 atcagagaaa ccttaccttc ggctaccgtt gccagcacga taattgtcga ccccagctga
     1621 ttttttaaat cgtcaaccat ggtacgcaac attttcggct caacaccgct aagctcgcta
     1681 accaacagct taacaccatt aacatcaatt gccttactgg aaagatttgc gctctcctgt
     1741 gcggcagctt gttctttaag ctgttgtagt tctttttcta actgacgcgt acgttccagt
     1801 actgagcgca ctttatccgc cagattattg ctatcgcctt tcagcagatg cgcgacttcg
     1861 cttaagcgat cgctgtctgc atgaacggtg gtgatagcgc cttctccggt taccgcttcg
     1921 atacgacgaa cgcctgcagc agtacccgat tcagagatga tgcggaacag accaatatca
     1981 ccagtgcggc tggcgtgagt accgccacac agctcggtgg agaaatcgcc catgctcagc
     2041 acgcgtacac gctcatcata cttctcgccg aacagcgcca tcgcaccttt cgctttcgcc
     2101 gcttcgagat ccatgatgtt ggtttcgatc ggcaaattgc gacgaatctg tgcgttcacc
     2161 aggtcttcga ccgcacgaat ctcttccggt ttcatcgctt cgttgtgtga gaagtcgaag
     2221 cgcagcacct tgtcgttaac cagtgaacct ttctgcgata catgagtccc cagaacctgg
     2281 cgcagcgcag cgtgcatcag gtgcgttgcg gagtgattca gacgaatacg ggcgcgacga
     2341 gcctcatcaa catcagcctg cacagcgtcg cccactttca gagaacccgc agcaagttta
     2401 ccgatgtgac caatcgcctg gccgtatttc tgcgtatctt ccaccgcgaa ggagaagtta
     2461 gcgcctttca gttcgccttt atcaccaacc tgaccgccgg attccgcata gaatggtgtt
     2521 tgatccagca cgaccacagc ttcctggcct gcattgatgg catcaaccgc tttaccatca
     2581 acaaacagcg cggtcacttt gccgttcagt tccagatggt catagccttt aaattcagat
     2641 gcactatcaa cacggatcat tgcgttgtaa tcggcaccaa agccgctggc ttcgcgcgcg
     2701 cgacgacgct gctcttccat tgctgcttca aaaccagctt cgtcaacttt gatgttgcgc
     2761 tcacgacaaa catcagccgt caggtcaacc gggaagccat aggtgtcgta cagacggaaa
     2821 gccgtttcac catccagcgt atcaccagaa agttttgcca gctcttcatc cagcaacgcc
     2881 agaccgcgct ccagagtacg ggcaaactgc tcttcttcag tcttcagcac ctgctcaacc
     2941 tgcgcctgct ggcgtttcag gtcttcaccc gcagagccca taacgtcgat cagcggacca
     3001 accagtttgt agaagaaggt ttctttcgcg ccgagcatat tgccgtgacg cactgcgcga
     3061 cgaatgatac gacgcagtac ataaccacgg ttttcattgg acggcattac gccatccgcg
     3121 atcaggaacg cacaagaacg aatgtggtca gcgattacgc gcagcgattt attgctcaga
     3181 tcggtcgcgc cagtgacttt cgctaccgcc tggatcaacg tgcggaacag gtcgatgtca
     3241 tagttagagt taacgtgttg cagcaccgca gcaatacgct ccagacccat accggtatct
     3301 acagacggct tcggcagcgg ttccatcgtg ccatctgcct ggcggttgaa ctgcatgaag
     3361 acgatgttcc agatctcaat gtagcggtcg ccgtcttctt ccggacttcc cggagggcca
     3421 ccccaaatgt ggtcgccgtg atcgtagaag atttcggtgc acgggccgca cggaccagtg
     3481 tcacccatct gccagaagtt gtcagatgcg tatggcgcac ctttgttatc gccgatgcga
     3541 ataatacgtt cgcgcgggat cccgacttct ttttcccaga tttcgtaggc ttcgtcgtcg
     3601 ctttcataga cggtaaccca cagacgctct ttcggcaggg caaaccattt ttcgctggtc
     3661 agcagttccc atgcaaactg aatggcatcg tgtttgaaat agtcgccgaa gctgaagttg
     3721 cccagcattt cgaagaaggt atggtgacgc gcggtgtaac cgacgttttc caggtcgttg
     3781 tgtttaccac ccgcacgcac gcagcgttgg gaagtggtag cgcgggaata attacgcttg
     3841 tcgagcccaa ggaacacatc cttgaactgg ttcatcccgg cgttggtaaa caacaaagtt
     3901 gggtcgttat ggggtaccag ggagctgctg gcaactacct gatgtccctt actatggaaa
     3961 aagtcgagaa acgcctgacg gatctcagcg gtgctcttgc tcataattat cctgaaatca
     4021 agctaacgaa atatcgccac cagctccagc gtgtcttaac cgccgggctg gtaactgaaa
     4081 agtgggaata agataagttt tcttgactgg gaagtaaaat accgtatgcg ttcagtcggc
     4141 aaaatttcgc caaatatcct ggatatcttc catcagatag ccacgataga gcagaaaacg
     4201 ctggatctta actttttctg aaaagacagt tggcaaaggt tcaccatatt ttcgcgtcgc
     4261 ctgatcgcgc gccagtgcgc accagtcgat gtcacattca cgcatcgctt tttctgtcgc
     4321 ttcgcgggaa atacctttct gattcagttc ctgacgaata cgtgcaggtc cataaccttt
     4381 gcggctacgg ctggcgataa agcgcgcaac aaatcggctg tcatcgagat agccatgttc
     4441 atggcaccag gcaataacgc gctcgtaatc ttctgccgta gcatcaatct cttctgggcc
     4501 atttttgccc ataatcggtg ccacgagttt acgtcgcagt tcttgctcac tgtgatcgcg
     4561 caccgccaga atgcgtaccg cacgatccaa caggcgagca tatgccgggc gacgggacgt
     4621 tgattctgtc atggcatatc cttacaactt aaaaaagcaa aagggccgca gatgcgaccc
     4681 aggttttgct gataaacaga tttaatagtc aatgagtgtg cagaatgttc ccgcaggccc
     4741 aattcgttag aggatcgagg tgagagtaat ttttcacgcc agtgctggcc tgttcccctc
     4801 accctaaccc tctccccaaa ggggcgaggg gaccgtccgt gcacacatta tctattatca
     4861 tcaataacga acgattaaaa atcttcgtta gtttctgcta cgccttcgct gtcatctaca
     4921 gagaaatccg gcgttgagtt cgggttgctc agcagcaact cacgtacttt cttctcgatc
     4981 tctttcgcgg tttccgggtt atctttcagc caggcagttg cattcgcttt accctgaccg
     5041 atcttctcac ctttgtagct gtaccacgcg cctgctttct cgatcagctt ctcttttacg
     5101 cccaggtcaa ccagttcgcc gtagaagttg ataccttcgc cgtagaggat ctgaaattca
     5161 gcctgtttaa acggtgcagc gattttgttc ttcaccactt tcacgcgggt ttcgctaccc
     5221 accacgtttt cgccctcttt caccgcgccg atacgacgga tgtcgagacg aacagaggcg
     5281 tagaatttca gcgcgttacc accggtagtg gtttccgggt taccgaacat cacaccaatt
     5341 ttcatacgga tctggttgat gaagatcagc agcgtgttgg actgcttcag gttacccgcc
     5401 agcttacgca tcgcctggct catcatacgt gccgcaaggc ccatgtgaga gtcgccgatt
     5461 tcgccttcga tttccgcttt cggcgtcagt gccgccacgg agtcaacgac gataacgtct
     5521 actgcgccag aacgcgccag ggcgtcacag atttccagtg cctgctcgcc ggtgtccggc
     5581 tgggagcaca gcaggttatc gatatcgacg cccagtttac gtgcgtagat tgggtccagc
     5641 gcgtgttcag catcgataaa cgcacaggtt ttaccttcac gctgcgctgc ggcgatcacc
     5701 tgcagcgtca gcgtggtttt accggaagat tccggtccgt agatttcgac gatacggccc
     5761 atcggcagac cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt agagatggtt
     5821 tccacatcca tggaacggtc ttcacccagg cgcatgatgg agcctttacc aaattgtttc
     5881 tcaatctggc ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat agccattttt
     5941 actcctgtca tgccgggtaa taccggatag tcaatatgtt ctgttgaagc aattatactg
     6001 tatgctcata cagtatcaag tgttttgtag aaattgttgc cacaaggtct gcaatgcata
     6061 cgcagtagcc tgacgacgca ccgcatcacg gtcaccgctg aagcattccc gccgggtaat
     6121 gccttcaccg cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct tctcttcact
     6181 gccgccatcc ggcccggcga taccactaat agacacggca taatcagcac gagccgcttt
     6241 cagtgcacct atcgccattt ccaccacgac gggttcactc accgcgccat gctgcgccag
     6301 cgtctcttcg cgtacgccga tcatctgcgc tttggcttcg ttactgtagg tgacaaatcc
     6361 gcgttcaaac caggcggagc taccggcaat atcggtaatc actttcgcta cccaaccacc
     6421 ggtacaagac tcggcagttg ttacggttgc gccacgggct ttcagcgcct gcccaacctg
     6481 ttcacttaac tgcatcagtt cactgtcagt catcacaacc tctgtcagtc aaaaatttat
     6541 gccggacaag atagcacttt cgtcaggaag atggtgttgg cgttgcaatt ttacgaggag
     6601 gattcagaaa aaagctgatt agccagaggg aagctcacgc ccccctcttg caaatagtta
     6661 ctgcacgcgc gccagcgcca cggcttgtcc taactgccag accgccattg cgtaatgggt
     6721 gctgtggtta taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt actggtaacc
     6781 ggtaccgaca tccagacgca gcaggctggc ttgttgatgg ttgcccagcg gctgctgtgg
     6841 cgttaaacct gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc catttggcaa
     6901 gcctggagcc tgaccgtttg ccattaccgc gacctgatcg cctttcaccc agccgtgcgc
     6961 tttgaaatag ttcgccacgc taccgatcgc atcaaccgga tcccacaggt tgatatgccc
     7021 gtcgccgctg aaatctaccg catattgttt gtaagacgac ggcataaact gtccgtagcc
     7081 catcgccccg gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat
     7141 caacaggaag gtttccagct cgccagagaa atactccgcg cggcgtgggt agttaaatga
     7201 cagcgttgcc agcgcatcga ggatgcgagt tttccccatc acgcgccccc agcgggtttc
     7261 aacgccgata atcccgacga taatttccgg cggtacgcca tacacctgcc acgcgcgatt
     7321 caacgcatct tcatactgat tccagaaaac cacaccgttc tgcacgttgt ccggcgtaat
     7381 aaattttttg cgataacgga gccatgcgcc gttcggacct gatggcggtt tcaccgatgt
     7441 ggttggtgcc tggttatcca tcagccgcag taccgaatcc agacgcttcg cctgggagag
     7501 aatttcctgc aactgctgac gatcgaaacc gtgtttgttc accattttgt cgatgaactg
     7561 ctgggcattc gggttattag cgaaatcgcc gcccatctgc atcacattgt gctgcggctc
     7621 aagcaggaag ccgccagacg gcgttccggt ggtcgtttca gtctcagtag gttttggctt
     7681 gctgctacag gcggcaagca acacaaaaag gggaagcaat gttacataac gacgcttgaa
     7741 catgaggggt ccatttaaca gattcaacca ggggcaagta tggtaaagca tcacgcccca
     7801 cacaaggaag cggtagtcac tgcccga
//
LOCUS       DAFNBJ010000199         7818 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000199 DAFNBJ010000000
VERSION     DAFNBJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.199"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..178)
                     /locus_tag="L2X49_004351"
     CDS             complement(<1..178)
                     /locus_tag="L2X49_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HBN4799338.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVA"
     gene            complement(383..1087)
                     /locus_tag="L2X49_004352"
     CDS             complement(383..1087)
                     /locus_tag="L2X49_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBN4799339.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1224..1676)
                     /gene="moaE"
                     /locus_tag="L2X49_004353"
     CDS             complement(1224..1676)
                     /gene="moaE"
                     /locus_tag="L2X49_004353"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBN4799340.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1678..1923)
                     /gene="moaD"
                     /locus_tag="L2X49_004354"
     CDS             complement(1678..1923)
                     /gene="moaD"
                     /locus_tag="L2X49_004354"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBN4799341.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1916..2401)
                     /gene="moaC"
                     /locus_tag="L2X49_004355"
     CDS             complement(1916..2401)
                     /gene="moaC"
                     /locus_tag="L2X49_004355"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBN4799342.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2404..2916)
                     /gene="moaB"
                     /locus_tag="L2X49_004356"
     CDS             complement(2404..2916)
                     /gene="moaB"
                     /locus_tag="L2X49_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBN4799343.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2938..3927)
                     /gene="moaA"
                     /locus_tag="L2X49_004357"
     CDS             complement(2938..3927)
                     /gene="moaA"
                     /locus_tag="L2X49_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBN4799344.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3915..4059)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4324..5232
                     /gene="yvcK"
                     /locus_tag="L2X49_004358"
     CDS             4324..5232
                     /gene="yvcK"
                     /locus_tag="L2X49_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBN4799345.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5424..7445)
                     /gene="uvrB"
                     /locus_tag="L2X49_004359"
     CDS             complement(5424..7445)
                     /gene="uvrB"
                     /locus_tag="L2X49_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBN4799346.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1894 a         2059 c         1937 g         1928 t
ORIGIN      
        1 tggcgacata cattgttggt gaatcatatt tggttgccgt aatttcgaga tacttagctc
       61 cccaggccag tcctgcggtt gccaccaaac ctaaagcggt aatgagccat ataccgttta
      121 atatcctggt ctcgtgctga aaatctaatt tattgctgtt ttgtgagtat gattccattc
      181 ttttagttgt ccttagaaaa aatattaata ttcaccatca ataaatttaa aaccacgcat
      241 acaaaatact tttgtattta tggatacgga aaacgcttcc gctcgccata aggcgtattt
      301 tatacctggt acttgttaag tgtcgttttt agtaagaaaa aaaatcccga cacatggccg
      361 ggatattgat ggtgaaaaag aattaacggc ggttgccaaa aattcgcagc aacatcagga
      421 acaggttgat gaagtccaga tacaaggtta acgcgccaag aatggaatat ttgcgcaggt
      481 tcgacgtgtc gcgggtatca atctgctcac ccatattttt caatttctga gtgtcatacg
      541 ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac tgcccacatc aatgcttcgc
      601 ttttcagcca gaagttgacc agcgatgcca gcacaatgcc gattaacgcc ataaacagca
      661 tattgccaaa gccacttaaa tcgcgcttcg tggtgtaacc gtacaggctc attgcgccga
      721 acatcccggc agtaacgacg aaagtactgg cgatagaagc agcggtatag acaatgaata
      781 tactggaaag cgtaagaccc gtcagcgccg aataaagcat aaagagcatc gtcgttacac
      841 ctgcgctcag cttttgaatc atcgctgata acacaataac caatgctaat tgcgcgatga
      901 tcagaccgat taaaaagaca cggttagtaa acaacagctc catcacggcc gcggaattag
      961 ccgcatacca ggcaacaaat gcggtcagca acaagccaac ggtcatccag ccatagactt
     1021 gcgccatata agtttgcaag ccagcccggg gttgtacgat tgaatcagaa cgtgggaatc
     1081 tgtccatgac gatctcctga agatataagg aatatcttaa ggatactgca aaatgatgag
     1141 gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt gaaattgccg gacgcagcgg
     1201 tgccttatcc ggctaacaaa aaactaccag cgttttgccg cctgctgatc gctctcccga
     1261 gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct tccagaacgg tgcgcgggtt
     1321 ttgagataat ccataataaa ctgcccggct tcaaacgcac tgctgcgatg cgcactggtg
     1381 acaccgacaa aaacgatttc atcgcccggc cataattccc cgatgcggtg aatcacagtg
     1441 acgcgcccca gcggccagcg gttacgcgct tcatcaacaa tttctgccag tgctttttca
     1501 gtcatccccg gatagtgttc gagggttaat gctttgacgc tgtcgcccag gttatggttg
     1561 cgcaccttac cagtaaaggt gactaccgca ccgtcttcgt cacgctccgc cagccacggg
     1621 tactcttctc ctacgctgaa cggctgcgga ccaacaacaa ttttggtttc tgccatctta
     1681 acctccggtt accggcggga agaaagctac ttcgtcgccg tcagtcagcg gatggtcaaa
     1741 actcaccagc gtctggttga cggcagccag taatttgcca tcttccagcg ccagcgccca
     1801 gcgatcgctc tgcgcagcca tgtgctggcg taacgcttca acagttggga aatccgcagc
     1861 cacttcggtt gcatctgttc ccaccaactc gcgcacctgg gcgaaaaaaa gaactttaat
     1921 catccgcttc caccttaaag tcacccgact tgccgccgct cttcgccagc aaacgtaccg
     1981 gaccaatcac catatctttt tgcaccgctt tgcacatgtc ataaatggtc agcgccgcca
     2041 cggaggccgc ggttaatgct tccatttcga caccggtttt cccggtcagg cggcataagg
     2101 tttctatacg cacccgattg tgctccggct cggcctgtaa attgacttca actttgctga
     2161 gcatcagcgg atgacagagc gggatcagat cccaggtgcg ttttgccgcc tgaataccgg
     2221 caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg accatcaata atcatcgcca
     2281 gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc ttcacgcacg gtttccgctt
     2341 tggcggagac atccaccatg tgcgcttcgc cagcggcgtt gatatgggtc agttgcgaca
     2401 tacttatttc ttcaaatgtg gatggaaatt acacggacgc gtacgggcat ccagctgcgg
     2461 cgcgatgata ttttcccatg cggtacggca cgctttggtc gaacccggca tggcgaaaat
     2521 cagcgttttg ttggcgacgc ccgctaccgc acgagattgc aacgtggaag tgccaatctc
     2581 ttcaaacgac aacatacgga acacttcacc aaaaccttca atttcacggt cgaacaacgg
     2641 cagcaatgct tcgggagcct gatcaccttc cgtcaggcca gtaccacccg taatcaatac
     2701 cacttgtaca tcgtcgctgg cgatccacgc agatacctga gcgcgaatag cgtagcggtt
     2761 ttctttcaca atggctttat cgacaacgtg atggcccgct tcttgcgccg aatcgcgcag
     2821 atagtgaccg gaggtatcgt cttcttcacc gcgacgatta gaaaccgtaa gaatagcaat
     2881 acgggtcggg ataaattcag tgcttacctg actcatctga tctctccttt tgacgtttta
     2941 gccgccaatg tacgataagt tttgcgtaat accggtgttg ttttgatgca ggaaatgggt
     3001 ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc agcgcctgtt gctgggtatc
     3061 gtcttccagc agatcgcgca ggttaacgcc gccttcacca aacaggcaga gatggagttt
     3121 accaatggag gaaacgcgca ggcggttgca agtggcgcag aagtcttttt catacggcat
     3181 gataaggcca atctctccgg cgtaatctgg atggcaaaag acttgcgcgg gaccgtcgct
     3241 gcgttgacgt aattggtgga tccagccgcg acgcagtagc tcgtcacgca gaacctgacc
     3301 agagatgtga tgcttacgga agagctcgct gccctcgccc gtttccatca gttcgatgaa
     3361 acgcagctgg atagggcgat gctggatcca gttcagaaag gtgtcgagct ggtgatgatt
     3421 aacatcacgc atcagcacgg tattgacttt gaccttctca aaaccggcct caaatgcagc
     3481 atcaatcccc gccatgacct ggttgaattt atcctgcccg gtaatagcgt gaaactggcg
     3541 ggcgtccaga ctgtcgacac tgacgttaat gccagtaagt cccgcatcgc gccagttcgc
     3601 cacatcgcgt tccagacggt aaccattggt ggtgaccgca atctggcgga tagcgtcgtt
     3661 ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt aaagacggct caccgcctgt
     3721 caggcgcact ttttcggtgc ccagactggc gaaggcgcgc gtaacccggc gaatttcatc
     3781 gacggtaaga aagcctttat tggtgacgcc gctcggtttg tagccatccg gcaggcagta
     3841 ggtgcaacga aagttacaca catcggtaat cgacaggcgc aagtagtaaa acttacgcgc
     3901 aaatgcatca gtcagttgtg aagccatgta cacctttcca gatacgggag gcgaagtcat
     3961 ttcttccttc gccctggtgg caatctttac gagcattgtc acggccaaag caccgtatca
     4021 gttgacccag gtgcagaggc tagagtgttt agtggttatg ccgatactag cgtgtaaatg
     4081 agatttttac catccacact ttcgctatgt aatcatgtat atagcgtcat gatcgtgcta
     4141 tttcgctaca gaataaggga aaatggcggc tttactttga tatacatcat cagggaggca
     4201 tagcgtgcat cgtcctttag aggtagaaga aaatagtttg tatcctcaat attggcaggt
     4261 taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg ataatctgac tccaggaaac
     4321 tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg ttgctctcgg cggagggcat
     4381 ggactgggac gcgttctctc atcactttcg tctttgggtt ctcgtttaac gggtatcgtc
     4441 accaccaccg ataatggtgg ctcgacgggg cgtattcgcc gttcagaagg cggcattgcc
     4501 tggggcgata tgcgcaactg cctcaaccag ctgataacgg aaccgagcgt cgcctccgcg
     4561 atgtttgaat accgttttgg tggcaatggc gaactttccg gtcataatct cggaaacttg
     4621 atgttaaagg cgctggatca ccttagcgtg cggcctctgg aagccatcaa tttaattcgt
     4681 aatctgctga aagtggatac gcatttgatt ccaatgtcag agcatcctgt tgatctgatg
     4741 gcgattgacg atcaggggca tgaagtttac ggcgaggtca atatcgacca gttaactacg
     4801 ccgattcaag agttattgtt aacgcctaat gtacccgcaa cgcgtgaggc ggttcacgct
     4861 atcaatgaag cggatctcat cattattggg cctggcagtt tttataccag cctgatgcca
     4921 attctgctgc tgaaggaaat cgcccaggca ttacgccgca cgccagcgcc gatggtttat
     4981 atcggcaatc tggggcgtga gttgagttta cctgcggcta atttgaagct ggaaagcaag
     5041 ctggcaatta tggagcagta tgttggtaaa aaggtcattg atgcggtcat cgtcgggcca
     5101 aaagtggatg tctcggcggt gaaagagcgg attgtgatcc aggaggtact ggaggccagc
     5161 gatattccgt atcgtcatga ccgccagttg ttacataacg cgctggaaaa ggcgttacag
     5221 gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc ctattgaaga caaagtgcgc
     5281 gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccgc gcaaattcaa
     5341 tatattgcgg agaaaatgta ggcctgataa gcgtagcgca tcaggctgtt ttccgtttgt
     5401 catcagtctt cttcgctatc ctgttacgat gccgcgataa acagctcacg cagctgatgc
     5461 aactggtcac gaatttgcgc cgcttcttcg aactccagat tctgcgcgtg ttgcatcatc
     5521 aacccttcca gctcatggat tttctgctgc aacgctttag gcgacatatc catcggcaca
     5581 ttatccggct caacaatcgg tcgcgatttt cctctgccct tcgctttggt tttagcaatg
     5641 ttctgcccca acgccaggat atcgaccact ttcttgttca agccttgcgg cgtaatgccg
     5701 tgttcctcgt tgtacttctg ctgtttctcg cggcgacgtt cggtttcgcc aatcgctttc
     5761 gccattgatg gggtgatctt atcgccgtag agaatcgctt taccgttaac gttacgtgcc
     5821 gcacgaccaa tggtctggat caacgaacgt tcggaacgca ggaagccttc tttgtcagcg
     5881 tcgaggatcg ccaccagcga aacttccggc atatccagac cttcacgcag taagttgatc
     5941 cctaccaata cgtcgaactc acccagacgc aagtcgcgga taatctccat acgctcgacg
     6001 gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt gttcttcgag atattcggta
     6061 agatcttccg ccattcgctt ggtcagtgtg gtgaccagta cgcgttcgtt aattgccgct
     6121 cgctgacgaa tctccgaaag aagatcatca acctgtgtcg ccaccggccg cacttcgata
     6181 atcgggtcaa gcaatccggt tggacgcacc acctgatcca ccacatcgcc gccggatttt
     6241 tccagctcgt aattacccgg cgtcgccgaa acatagatgg tttgcggcgc taatgcttcg
     6301 aactcttcaa acttaagcgg acggttatcc agcgctgatg gcaggcggaa gccgtactcc
     6361 accagtgtct ctttacgcgc ccggtcaccg cgatacatgc cgccaatttg tggaatggtg
     6421 acgtgagatt catcgaccac cagcagccca tcagcaggga ggtaatcaaa cagcgtcggc
     6481 ggtggctcac ccggtccacg accggagagg aagcgcgagt agttttcaat ccccgaacag
     6541 tagcccagct cgttcatcat ctccagatca aactgggtac gctgggtcag ccgctgctct
     6601 tccagcagtt tgttgttttc caacagcact ttgcgtctgg cggccagctc ttctttgatc
     6661 tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt agtgcgtttt cgggtagatg
     6721 gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg ggtcaaataa cgacaatcgt
     6781 tccacttcct cgtcaaacag ttccacgcga agtgcaatgt catccgattc tgccgggaag
     6841 atatctatca cctcgccacg aacgcggaaa gtaccacgct ggaatgcttg atcattacga
     6901 gcgtattgca gctccgccag tcggcgcaga atcgcgcgct gatcgataat cataccgacc
     6961 gtgagatgga gcatcatctt gagatataaa tcaggatcgc ccagaccata aatcgcggaa
     7021 acagacgcca ccacaaccac atcacgccgc tccagcatcg ctttggtggc ggacaaacgc
     7081 atctgctcaa tatgttcgtt aaccgaggca tctttctcaa tgaaagtgtc ggaactcggt
     7141 acataggctt ccggctgata gtagtcgtag taggaaacga aatattccac cgcgttttcc
     7201 gggaagaact ctttcatttc gccatacagc tgggccgcca gcgttttgtt gggcgcaagt
     7261 accatggttg ggcgctgaag gtcagcaatg acattggcaa tggtgaaggt tttcccggag
     7321 ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt ccagcccctc ttcgagacgt
     7381 cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg aattcagttt gaacggttta
     7441 ctcatgagtc gctacctgaa ggagttgggc gggcaggtat gtaattttac tcgtcgtact
     7501 taattatgcc aacaaattat actggataaa aaaacagttc atcaccataa tatttctgtt
     7561 acagcgtaaa ctccgctgtc aatcatgagc aaaatttact ctgtggcgag ataaaactcc
     7621 ggccttaccg ggttatcccc aaagcaacgg cttttttaac atttgtcaag atgagtgatg
     7681 acagttttat ggcaagagat gcctgttcag tgactgccat tgcatttatc taaccagtta
     7741 aaaaataaaa gatatttctt tgagccgtct ttaacgccaa ttcgcgtgca agccgcgtat
     7801 tctctcgctt gcctcgtg
//
LOCUS       DAFNBJ010000200         7803 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000200 DAFNBJ010000000
VERSION     DAFNBJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.200"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            42..131
                     /gene="kdpF"
                     /locus_tag="L2X49_004360"
     CDS             42..131
                     /gene="kdpF"
                     /locus_tag="L2X49_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBN4799347.1"
                     /translation="MSAGVITGVLLVFLLRGYLVYALINAEAF"
     gene            131..1804
                     /gene="kdpA"
                     /locus_tag="L2X49_004361"
     CDS             131..1804
                     /gene="kdpA"
                     /locus_tag="L2X49_004361"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBN4799348.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1827..3875
                     /gene="kdpB"
                     /locus_tag="L2X49_004362"
     CDS             1827..3875
                     /gene="kdpB"
                     /locus_tag="L2X49_004362"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBN4799349.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMCLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3884..4456
                     /gene="kdpC"
                     /locus_tag="L2X49_004363"
     CDS             3884..4456
                     /gene="kdpC"
                     /locus_tag="L2X49_004363"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBN4799350.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLDNNIPPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVINIVELNLALDKLDE"
     gene            4449..7133
                     /gene="kdpD"
                     /locus_tag="L2X49_004364"
     CDS             4449..7133
                     /gene="kdpD"
                     /locus_tag="L2X49_004364"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HBN4799351.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRTQGLDIVVGVVETHGRKDTAAMLEELAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPDLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7130..>7803
                     /gene="kdpE"
                     /locus_tag="L2X49_004365"
     CDS             7130..>7803
                     /gene="kdpE"
                     /locus_tag="L2X49_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBN4799352.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDRIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1579 a         2055 c         2348 g         1821 t
ORIGIN      
        1 ctacccttcc ggtatcactt ttaggccact ggaggtgcac tgtgagtgca ggcgtgataa
       61 ccggcgtatt gctggtgttt ttattacggg gttatctggt ttatgccctg atcaatgcgg
      121 aggcgttctg atggctgcgc aagggttctt actgatcgcc acgtttttac tggtgttaat
      181 ggtgctggcg cgtcctttag gcagcgggct ggcgcggctg attaatgaca ttcctcttcc
      241 cggtacaacg ggcgttgagc gcgtactttt tcgcgcactt ggcgtctctg accgtgagat
      301 gaactggaag caatatctct gtgccattct cggcctgaac atgctggggc tggcggtgct
      361 gttttttatg ttgctcggtc agcactatct gccgcttaat ccacagcagt tgccggggct
      421 gtcgtgggat ctggcgctga ataccgccgt cagctttgtc acgaatacca actggcaatc
      481 ttacagcggt gaaaccacgt tgagctattt cagccagatg gctggcttaa cggtgcaaaa
      541 ctttctttct gccgccagcg ggattgcggt gatttttgcc ctcatccgtg cgtttacccg
      601 ccagagcatg agcacactcg ggaatgcctg ggtcgatctg ctacgcatca cgttatgggt
      661 gctcgtccct gtggcgttgt tgatcgcact gttttttatt caacaaggtg cgctgcaaaa
      721 ctttcagcct tatcaggctg tgaataccgt tgaaggagcg caacagctgt tacccatggg
      781 gcctgtagct tctcaggaag cgatcaagat gctcggtact aacggcggtg gcttctttaa
      841 tgccaactcg tcgcatccgt ttgaaaaccc aaccgcactg accaacttcg tgcagatgct
      901 ggcgatcttc ttgatcccaa cggcgctgtg ctttgccttt ggtgaagtga cgggcgatcg
      961 ccgccagggg cgcatgttgc tgtgggcgat gtcagtgatt tttgtcatct gcgtaggcgt
     1021 ggtgatgtgg gcagaagttc agggtaatcc tcatctgctg gcgctcggcg cagacagcag
     1081 catcaatatg gaaggtaaag agagccgttt cggcgtgctg gtcagtagcc tgtttgcggt
     1141 cgtgactacg gcggcttcct gtggcgcggt gattgcgatg catgattcgt ttaccgctct
     1201 cggtggcatg gtgccgatgt ggctgatgca aattggtgaa gtggtgttcg gcggtgtcgg
     1261 ttctggtctt tacggcatga tgctgtttgt cctgctggcg gtgtttattg ccgggctgat
     1321 gattggtcgt acaccggaat atctgggtaa aaaaatcgac gtacgcgaga tgaaactgac
     1381 tgcactggcg attctggtta ccccgacgct ggtgctgatg ggcgcggcgc tggcaatgat
     1441 gaccgacgcc ggacgtagcg ccatgctcaa ccctggcccg catggtttta gcgaagtgct
     1501 gtacgccgtg tcatccgccg ctaacaacaa cggcagcgcc tttgccggat taagcgccaa
     1561 ctctccgttc tggaactgtt tactggcgtt ctgcatgttt gtcggtcgct tcggggtgat
     1621 tatcccggtg atggcaattg ccggttcgct ggtgagtaaa aagagccaac ccgccagctc
     1681 cggcacgctg ccaacgcacg gcccgctgtt tgttggcctg ttaatcggca ccgtgttgct
     1741 ggttggcgca ctgaccttta tccctgccct ggcgcttggt ccagtggcgg aatatctctc
     1801 ctgatgatat tgagtgagca ctgaatatga gtcgtaaaca actggcgcta ttcgaaccaa
     1861 cacttgtcgt tcaggcgctg aaagaagcgg tgaaaaaatt aaacccgcag gcgcaatggc
     1921 gcaatccggt gatgtttatc gtctggatcg gcagtctgct gaccacctgt attagcatcg
     1981 cgatggcaag cggtgcgatg cctggcaatg cgctgtttag cgcggcaatt agcggctggc
     2041 tgtgggtcac cgtactgttc gctaatttcg ccgaggcgct ggcagaaggc cgcagtaaag
     2101 cgcaggccaa cagtcttaaa ggggtgaaaa aaactgcctt tgcccgcaag ctgcgtgagc
     2161 cgaaatatgg cgctgcggcg gacaaagttc ctgccgatca actgcgtaaa ggtgacatcg
     2221 tgctggtaga agctggcgat attatcccct gcgatggtga agtcattgaa ggcggcgcat
     2281 cggtcgatga aagcgccatc accggggaat cggcaccggt gatccgtgaa tccggcggcg
     2341 attttgcctc cgtcaccggc ggcacgcgta ttctttctga ctggctggtg attgagtgta
     2401 gcgttaaccc cggtgagaca tttctggatc ggatgatcgc gatggtggaa ggcgcacagc
     2461 gacgcaaaac accgaacgag attgccctga ctatcctgct gattgctctg accatcgtct
     2521 ttttactggc aaccgccacg ctgtggccgt tttccgcgtg gggcggtaat gcagtcagcg
     2581 taacggtact ggtggcgctg ctggtctgtc tgatcccaac cactattggc ggcctgttgt
     2641 cagcgatcgg cgtcgccggg atgagccgga tgctaggcgc gaatgtgatt gccaccagcg
     2701 gacgtgcagt tgaagcggca ggtgacgttg acgttctgct actggataaa accggcacca
     2761 tcacactcgg taaccgtcag gcgtcggagt ttatccccgc gcagggcgtg gatgaaaaaa
     2821 cgctggctga cgccgcacaa ctggcttcgc tggctgatga aacgccggaa ggccgcagta
     2881 ttgtgatcct cgccaagcag cgttttaacc tgcgcgagcg cgatgtgcag tcgctccatg
     2941 ccacctttgt accgtttact gcgcaaagcc ggatgagcgg gatcaacatc gacaaccgca
     3001 tgatccgtaa aggttctgtc gatgccattc gtcgccatgt tgaggctaac ggtggtcact
     3061 tccctaccga tgttgatcaa aaagtcgatc aggttgcgcg tcagggagcc acgccgctgg
     3121 tggtggtgga aggttctcgt gtgctgggcg ttattgcgct gaaagatatc gtcaaaggcg
     3181 gtattaaaga gcgcttcgcc cagctgcgca aaatgggcat taaaacggtg atgattaccg
     3241 gcgataaccg tctgactgcc gccgcgattg ctgcggaagc gggtgtcgat gattttctcg
     3301 ccgaagcgac accggaggcc aagctggcat tgatccgtca gtatcaggcg gaaggtcgtt
     3361 tggtagcgat gaccggcgac ggcaccaacg atgctccggc gctggcgcag gcagatgtcg
     3421 cggtggcgat gaactccggc acccaggcgg cgaaagaggc gggcaatatg gtcgatctcg
     3481 actctaaccc gaccaagttg atcgaggtgg tgcacattgg caaacagatg ctgatgaccc
     3541 gtggctcgct gaccaccttc agcattgcca acgatgtggc gaaatacttc gccattattc
     3601 cggcggcatt cgcggcaacg tatccgcagt taaatgcgct gaacatcatg tgcctgcatt
     3661 cgcccgactc cgcaatcctc agtgcggtga ttttcaacgc cttgattatc gtctttttga
     3721 ttcccctggc gttaaaaggc gtgagttata aaccgcttac cgcttctgcc atgttgcgcc
     3781 gtaacttatg gatttacggt ctgggtgggc tgctggtgcc gtttatcggt atcaaagtca
     3841 ttgatttact gctgaccgtt tgcggtctgg tgtgaggttt accatgagtg gattacgtcc
     3901 ggcattatca acatttatct ttctgttatt gattactggc ggcgtttacc cgctgctgac
     3961 caccgtactg gggcaatggt ggtttccctg gcaggccaat ggttcgttga ttcgtgaagg
     4021 tgatacggtg cgcggttcgg cattaatcgg gcagaatttt accggcaacg gctattttca
     4081 tggtcgcccg tcggcaacgg cagaaatgcc ctataatcca caggcttctg gcgggagcaa
     4141 tctggcggtc agtaaccctg agctggataa actaatagcc gcacgcgttg ctgcattacg
     4201 ggccgctaac ccggatgcca gcgcgagcgt tccggttgaa ctggtgacgg catcggcaag
     4261 cgggctggac aataatatcc ccccgcaagc ggcggcctgg caaatcccac gcgtggcgaa
     4321 agcgcgtaat ctcagcgttg aacagctcac gcaactgatc gcaaaataca gccaacaacc
     4381 gctggtgaaa tatatcggcc agccggttat caacattgtt gaactcaatc tggcgctgga
     4441 taaacttgat gaataacgaa cccttacgtc ccgaccccga tcgtctgctg gaacaaactg
     4501 ccgcgccgca tcgggggaag ctgaaagttt tcttcggtgc ctgtgcaggc gtcgggaaga
     4561 cctgggcgat gctggcagaa gcacagcgac tgcggacgca agggctggat attgtggttg
     4621 gcgtggtaga aacccacggg cggaaagata ccgccgccat gctggaagag ctggctgttc
     4681 tgccgccaaa acgcctggcg taccgtgggc ggcatatcag cgagtttgat ctcgatgccg
     4741 ccctcgcccg ccgtccggcg ctgatcttaa tggacgaact ggcgcacagt aatgcgcctg
     4801 gttctcgtca tcccaaacgc tggcaggata ttgaagagct gctggaagca ggcattgatg
     4861 tttttactac cgtcaacgtt caacatctgg aaagcctgaa tgatgtggtc agcggcgtca
     4921 ccggaattca ggtacgggaa accgtgcccg atcctttttt cgatgccgcc gacgacgtgg
     4981 ttctggtgga cttgcccccg gacgatctgc gccagcggct gaaagaaggc aaagtctata
     5041 ttgccgggca ggcggagcgc gccattgaac attttttccg caaaggcaat ctgatcgccc
     5101 tgcgcgaact ggcactgcgc cgtactgccg atcgcgtaga tgagcaaatg cgcgcctggc
     5161 gggggcatcc tggcgaagag aaagtgtggc acacgcgcga cgcgatcctt ttatgcatcg
     5221 ggcataacac cggcagtgag aaactggtcc gcgcggcggc gcggctggcg tcgcggctgg
     5281 gtagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta ccggagaaaa
     5341 aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg gagacggcaa
     5401 cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa cataatctcg
     5461 gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa acgtttgctg
     5521 accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac
     5581 ccgcccgcac gattaacaac gcgccggata gccgctcttt taaagacaag tggcgggtac
     5641 agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacttta attgccatgc
     5701 agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt ggcgtggtgg
     5761 tagtggcgct attttatgga cgctggcctt cggtggttgc taccgtcatt aatgtggtga
     5821 gtttcgatct cttttttatc gccccacgcg gcacgctcgc cgtctctgat gtgcaatatc
     5881 tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat cgggaacctt actgctggcg
     5941 tgcgttatca ggcgcgggta gcccgttacc gcgagcaacg cacacggcac ttatatgaaa
     6001 tgtcgaaagc tctggcggtg ggccgcagtc cgcaggacat cgccgccacc agcgaacaat
     6061 ttattgcctc cacgtttcat gcccgcagtc aggtgttgtt gcccgatgac aacggtaaat
     6121 tgcagccgtt aacacatccg caaggaatga cgccatggga cgatgccatc gcgcagtgga
     6181 gttttgataa aggcctgcct gcgggtgcgg gcaccgacac gttacccggc gtgccgtacc
     6241 agattttgcc gctaaaaagc ggcgagaaaa cctacggact ggtggtggtg gaaccgggga
     6301 atctgcgcca gttgatgatc ccggagcagc agcgcctgct ggagacattt acgctgttag
     6361 tcgccaatgc ccttgagcgg ctgacactga ccgccagcga agaacaggcg cggatggcaa
     6421 gcgaacgtga acagatccgc aacgccctgc tggcggcgct ttcgcatgat ttacgcacgc
     6481 cgcttacggt gctgtttggt caggcagaaa tcttaacgct cgatctggca agcgaaggat
     6541 cgccccacgc ccgccaggcc agcgagattc gtcagcatgt gctgaacact acccgactgg
     6601 tgaataatct actggatatg gcgcgaattc agtccggcgg ctttaatttg aagaaagagt
     6661 ggttaacgct ggaagaagtg gtcggtagcg cgctgcaaat gctggaaccg gatttatcgt
     6721 cgcccatcaa tctttctctg ccagaaccgc tgaccttaat ccacgttgac gggccactct
     6781 ttgaacgggt gctgattaat ctgctggaga acgcggtgaa atatgcgggt gcgcaggccg
     6841 aaattggtat cgatgcccac gttgagggcg aaaatctaca actggatgtc tgggataacg
     6901 gccccggtct tccgccaggc caggagcaga cgatatttga taagtttgct cgcgggaata
     6961 aagagtcggc agtaccgggg gtagggcttg gactggcaat ttgtcgggcg atagtggatg
     7021 tacacggggg cactattacc gcgttcaacc gaccggaagg tggtgcctgt tttcgtgtta
     7081 cacttcccca gcaaaccgct cctgaacttg aagactttca tgaggatatg tgacaaacgt
     7141 tctgattgtt gaagatgaac aggctattcg tcgctttctg cgcacggcgc tggagggcga
     7201 cgggatgcgc gtctatgagg ccgaaacgct gcaacgcggc ttgctggaag cggctacccg
     7261 taagccagat ttgattattc tcgatctcgg cctgcccgat ggtgatagga ttgagtttat
     7321 ccgcgacctg cgccagtgga gcgcggtgcc ggtgattgtg ctttccgcac gcagcgaaga
     7381 gagcgacaaa atcgccgcgc tggatgccgg agcggatgat tatctgagta agccgtttgg
     7441 cattggcgaa ttgcaggccc gtctgcgcgt cgctttacgc cgccactccg ccaccgccgc
     7501 gcccgatccg ctggtgaaat tttccgatgt taccgtcgat ttagccgccc gcgtgattca
     7561 ccggggtgag gaagaggtgc atctcacgcc gattgagttc cgcctgctgg cggtgctgct
     7621 gaataatgcc gggaaagtgc tcacccagcg ccagttactc aatcaggtgt gggggccaaa
     7681 cgcggtcgaa cacagtcact atttgcgtat ttatatggga catctgcgac aaaaactgga
     7741 acaggatccc gctcgccccc gccatttcat tactgaaacc ggtattgggt atcggtttat
     7801 gcc
//
LOCUS       DAFNBJ010000201         7792 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000201 DAFNBJ010000000
VERSION     DAFNBJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.201"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(181..444)
                     /gene="cedA"
                     /locus_tag="L2X49_004366"
     CDS             complement(181..444)
                     /gene="cedA"
                     /locus_tag="L2X49_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBN4799353.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            627..2888
                     /gene="katE"
                     /locus_tag="L2X49_004367"
     CDS             627..2888
                     /gene="katE"
                     /locus_tag="L2X49_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBN4799354.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2935..3693)
                     /gene="chbG"
                     /locus_tag="L2X49_004368"
     CDS             complement(2935..3693)
                     /gene="chbG"
                     /locus_tag="L2X49_004368"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBN4799355.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVSRFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNLIRKSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3706..5058)
                     /gene="celF"
                     /locus_tag="L2X49_004369"
                     /gene_synonym="chbF"
     CDS             complement(3706..5058)
                     /gene="celF"
                     /locus_tag="L2X49_004369"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBN4799356.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5162..6004)
                     /gene="chbR"
                     /locus_tag="L2X49_004370"
     CDS             complement(5162..6004)
                     /gene="chbR"
                     /locus_tag="L2X49_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBN4799357.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6012..6362)
                     /gene="chbA"
                     /locus_tag="L2X49_004371"
     CDS             complement(6012..6362)
                     /gene="chbA"
                     /locus_tag="L2X49_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBN4799358.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6413..7771)
                     /gene="chbC"
                     /locus_tag="L2X49_004372"
     CDS             complement(6413..7771)
                     /gene="chbC"
                     /locus_tag="L2X49_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBN4799359.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNDLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
BASE COUNT         2111 a         2024 c         1735 g         1922 t
ORIGIN      
        1 atgattagca aaacttaacc attttaaaat aaaaaaacaa ttaaagaaaa aagatcactt
       61 atttatagca atagatcgtc aaaggcagct ttttgttact ggtggtttga atgaatgtag
      121 caatgaaata cagaatttca ggtcatgtaa ctcccggcaa aaccgggagg tacgtaatct
      181 ttactcagtc acttcccctt cctggcggat ctgatttgcc caacgctggg cggattcagg
      241 cacagtaaac gccggtgagc gcagaaatga ctctcccatc agtacgaacg caacatattt
      301 gccacgcagc atccagacat cacgaaacga atccatcttt atcgcatgtt ctggcggcgc
      361 gggttccgtg cgtgggacat agctaataat ctggcggttt tgctggcgga gcggtttctt
      421 cattactggc ttcactaaac gcatattaaa aatcagaaaa actgtagttt agccgattta
      481 gcccctgtac gtcccacttt gcgtgtattt cataacaccg tttccagaat agtctccgaa
      541 gcgggatctg gctgatggtc tatagttaga gggttttttg accaaaacag cggccctttc
      601 agtaataaat taaggagacg agttcaatgt cgcaacataa cgaaaagaac ccacatcagc
      661 accagtcacc actacacgat tccagcgaag cgaaaccggg gatggactca ctggcacctg
      721 aggacggatc tcatcgtcca gcggctgaac caacaccgcc aggtgcacaa cctaccgccc
      781 cagggagcct gaaagcccct gatacgcgta acgaaaaact taattctctg gaagacgtac
      841 gcaaaggcag tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc gccgatgatc
      901 aaaactcact gcgtgccggt agccgtggtc caacgctgct ggaagatttt attctgcgcg
      961 agaaaatcac ccactttgac catgagcgca tcccggaacg tattgttcat gcacgcggat
     1021 cagccgctca cggttatttc cagccatata aaagcttaag cgatatcacc aaagcggatt
     1081 tcctctcaga tccgaacaaa atcaccccag tatttgtacg tttctctacc gttcagggtg
     1141 gtgctggctc tgccgatacc gtgcgtgata tccgtggttt tgccaccaag ttctatactg
     1201 aagagggtat ttttgacctc gttggcaata acacgccaat cttctttatc caggatgcgc
     1261 ataaatttcc cgattttgtt catgcggtaa aaccagaacc gcactgggca attccacaag
     1321 ggcaaagcgc ccacgatact ttctgggatt atgtttctct gcaacctgaa actctgcaca
     1381 acgtgatgtg ggcgatgtcg gatcgcggga tcccgcgcag ttaccgcacc atggaaggct
     1441 tcggtattca caccttccgc ctgattaatg ccgaagggaa ggcaacgttt gtacgtttcc
     1501 actggaaacc actggcaggt aaagcctcac tcgtttggga tgaagcacaa aaactcaccg
     1561 gacgtgaccc ggacttccac cgccgcgagt tgtgggaagc cattgaagca ggcgattttc
     1621 cggaatacga actgggcttc cagttgattc ctgaagaaga tgaattcaag ttcgacttcg
     1681 atcttctcga tccaaccaaa ctgatcccgg aagaactggt gcccgttcag cgtgtcggca
     1741 aaatggtgct caatcgcaat ccggataact tctttgccga aaacgaacaa gcagcattcc
     1801 atccaggtca tattgttcct ggtctggact tcaccaacga tccgctgttg cagggacgtt
     1861 tgttctccta taccgataca caaatcagtc gtcttggtgg gccgaatttc catgagattc
     1921 cgattaaccg tccgacctgc ccttaccaca acttccagcg tgatggcatg catcgtatgg
     1981 ggatcgacac taacccggcg aattacgaac cgaactcgat caacgataac tggccgcgcg
     2041 aaacaccgcc gggaccgaaa cgcggcggtt ttgaatcata tcaggaacgc gtggaaggaa
     2101 ataaagttcg tgagcgcagt ccatcgtttg gcgaatatta ttcccatccg cgtctgttct
     2161 ggctaagtca gacgccattc gagcagcgcc atattgtcga tggtttcagt tttgagttaa
     2221 gcaaagtcgt tcgtccgtat attcgtgagc gcgttgttga ccagctggcg catattgatc
     2281 tcactctggc ccaggcggtg gcgaaaaatc tcggtatcga actgactgac gaccagctga
     2341 atatcacccc acctccggac gtcaacggtc tgaaaaagga tccatcctta agtttgtacg
     2401 ccattcctga cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat gatgaagtga
     2461 gatcggcaga ccttctggcc attctcaagg ccctgaaggc caaaggcgtt catgccaaac
     2521 tgctctactc ccgaatgggt gaagtgactg cggatgacgg tacggtgttg cctatagccg
     2581 ctacctttgc cggtgcacct tcgctgacgg tcgatgcggt cattgtccct tgcggcaata
     2641 tcgcggatat cgctgacaac ggcgatgcca actactacct gatggaagcc tacaaacacc
     2701 ttaaaccgat tgcgctggcg ggagacgcgc gcaagtttaa agcaacaatc aaggtcgctg
     2761 accagggtga agaagggatt gtggaagctg acagcgccga cggtagtttt atggatgaac
     2821 tgttaacgct gatggcagca caccgcgtgt ggtcacgcat tcctaagatt gacaaaattc
     2881 cggcgtaaat atggataagg tcacgtcccc tggggagtga ccttaccacc ttacttacac
     2941 atcaagataa ctacccaacc gataaccacg ctcagcaatt gcatatttca acgacgctga
     3001 tgtcagcact tccagctccg ttaaccgtgg gaaacagtaa gcactcttgc gaatcaaatt
     3061 atcgacaaac gccggatggc acatgacttc cagcgagcgt tccccgcgat ggctggaatc
     3121 atcaagcact tgcaggaaca gtgcctcgct aatctcttca ccataaaacg cgctactgaa
     3181 tccgtgcgaa ctgcgcagat tagccggtaa atcaccagcg ttagatagcg gctgacggtc
     3241 gatgcgcaac gcaatcccct cttcagccgc aaacctggaa acaataggga aaatctgcgg
     3301 gaacatatgc acatgatgat ggctatcaag atgcgtaggt ttgcgtccaa atagctcaat
     3361 gaaacgcaaa tactgactgg caagctcctg agtaatttct tccagcggta aagcatcttc
     3421 ttctgccaac tgccagatcc attttcccag cacaccatcg cgggttaacc ccggcatagc
     3481 tgtcagtggc ttgcccatag taaggacaaa gtgcatccct atggccagac tcggttcatc
     3541 acgactcaac tgcaccgcat ggtcaatagc ctgcccattc accagcgccg tcgtcgatgt
     3601 gacaatccca ttgcgacagg cctcgataat gccgtagttc tgacctttgc ttaagccaaa
     3661 atcatcggca ttaacaatca gtaagcgttc cataatcagc ctcggttaat gtgctttttt
     3721 aagctctgcg atgcagtcgg caaaatttgg cagccatttc tcgtgcgcca gaatcatctc
     3781 gcgtgccagc agctcagcat cgcgatcgga atgcaccaac ggactaaggt ttagcgccag
     3841 taacacatcg ttaaactctc cgctaagtgc ggcgttgctg gcagcaatct cgaagccttt
     3901 aatggtgtga atcagcccca tcactttatc atcgaaatgc gtaatgcgcg gatgtggcgt
     3961 cgcgccatcg cgccccagcg tacaggtcat ttctaccgcc cagtctgccg gaatattatc
     4021 aatatgccca tgatgcggga tattaacgta atgttctgct tgcttgtcgt tgtagatagc
     4081 gttgatcact tcgcacgctg catcagagta ataagcccca ccgcgctgtt ccagttcttt
     4141 cggcttaact ttcaactccg ggtttttata cagctcaaaa agttgtttct cgactttctg
     4201 tactacctgc gctcgtgcgc cgcctttgta gtattcgccc atttcaatag ccagcatctc
     4261 tttttgcttg aagtaataaa gcaaataaga acacggcaac agattcagag aacgaattaa
     4321 gccttcgcta aatggcagat cgaaaatatt tttaacgcca gatgctttta actgcccgga
     4381 tgccacacca tcaagtaatt cggcaaagcg agatttgcca tttaccagca catccttaat
     4441 gaacaccata tggttgaggc cgaacagatc gatagataaa tcatcgctgt ctttcagcat
     4501 cagaacatcg cgaataaaca tcttcatgcc gatcggaata ttacacacgc cgataaagcg
     4561 tttaaatccg gtatgacgat aaacggcttc agtgaccatt cccgccgggt tagtgaagtt
     4621 aatcacccat gcattcggac aaagttcttc gacatctttt acgatgtcaa aaatcaccgg
     4681 aatggtacgc agacctttaa acagaccgcc cgcgccgttg gtttcctgac caagataacc
     4741 atgacttaat ggaatacgtt catccagttc gcgcgccggt aattggccta cgcgcagttg
     4801 ggtagtaacg aaatcagcat ctttcaatgc ttcgcggcga tccagcgttt tataaagctt
     4861 catcgggacg ccagcgttat caatcatccg ttggcagaga tcgaaaataa tatccagttt
     4921 agctttgcca ccttcgacat ccaccagcca taattcgctg accggcaatt cgtgataacg
     4981 cttaataaat ccttccagta actccggggt atagctgctc ccgccaccaa tagtgacgac
     5041 ttttaatttc tggctcataa tttctccctt cagtacagaa tactgatatc tgacatatct
     5101 gccccccgga cataaataat ccagcaacag gacagatatg tgaattgtca ggtataacga
     5161 cttactgatt aaattcagtc aatttcttac gatagctctt aggcgtaaag gacgttaatt
     5221 tcttaaacgt tttaataaac aaactcgggc tactataacc ggcctcaaac gcaatatccg
     5281 tcactgaata gttggtcatt tccagttgtt ttttggcaaa attaatacgg atttcattaa
     5341 taatctgcat tggcgttttg ccataatatc gttgagtcgc tcgcgtcaaa tattcctgtg
     5401 atttggctga caacgtcacc atattctcca gcgccgattc actaaactgc tctttatcat
     5461 gcatcttttc taccgtactt ttcagccact gcggtacatc atcaatcacc tgttcttcgc
     5521 ggtaatgacg taaacggtta atgacataaa aagtaaccat ctcaacaaac tcttccagcc
     5581 ctgtttcgcg gaaattcaat gaagaaatca ctgtttccac ataggtgaga aaagcgttat
     5641 tggtccggta tacctgcgaa gctacaaagc aataaggcaa caacggcagg taatgctgct
     5701 caaaaaagcg tttactgatc ccaacgttca atatacgcgt ggcaccaaac tcataaaaac
     5761 tttgatggtg cgaacctaac ggaataaaaa caaaatcgcc ccgttccagt aacacgcgct
     5821 taccgttaat ctcctggaaa taacgcccgg ttaataccag agtaaattca taatagtcgt
     5881 gctgatgcag tccgctgata ctctcagttt tgttatagat aaacacatgg aagtttttgc
     5941 cattaaacaa ctgctgttct cgggcagtgg caatttccgg cgcgttaatc actggctgca
     6001 tcatcgactc cttatgcctt cagtttttca tgaagctcaa ttaattcagt aatcagttca
     6061 cgcgcaagca tggacgtcat taaatgatcc tgagcgtgga ccagcaccag actcactttc
     6121 atcttacctt cgcccgcatc gccttcaatc agtttcgtct gtaccagatg cgcttcattc
     6181 aatgccatcc gtgactgatc catcatggct tttgctgcgg caaaatcgcc ctgcttcgcc
     6241 tgtttcagtg ccgcatacgc caggctgcgc gcttgtccgg agttgatgat aagccccatc
     6301 accacttctt ccagctcttc agcttctgtt tgcgtatcgg gaatattatc gagatccatc
     6361 atacatcgtt cctctctttt cttaccggca cgattacccg taccggcatc gattaaaatt
     6421 tcagggcgtt agcgatatct tcttcgctct cttctttatc aatcgcgttt tgtgctttgt
     6481 tagccaccac aacaaagggc agataaatta acgttgcgat accaaggttg aagagtgcaa
     6541 ccagcaatgc ggcgacgcta ccgttggtgt taaagaaggc tcccagaccg gttggcatgg
     6601 tccacggtgc aatattggtc accggaggaa tgatgcccat gtagtacgct gcgagggtga
     6661 ttgccgccag aatcggttgt accagtacaa acgggataaa catcaccggg ttcatgataa
     6721 ttggcagacc aaacagaatc ggttcgttaa tctggaagat gccggacggc agcgccagtt
     6781 ttgccacctg acgataatca gcacggcgag aggcgataaa gatagccagg atcaggccta
     6841 aagtcgcacc actgccccca aggaaaataa aggagtccag catcggcttg gcccagatat
     6901 ggaaggtctt accggctgcc agcgccgctt cgacggaacc atattgctga taggtcgcga
     6961 tattttccag cgcccacggc gtcataatgc cgttgtccag tgcggtcagc gccagcgcgc
     7021 catgaatacc gaagaaccag agcagtggaa caaagatcac ataggcccag cccaccacgc
     7081 tacccaacga tgccagtggg gttgagatgg tatccataat gatctgatgg aagttggtgc
     7141 cccaggtatt caacgcccag gcaataatcc ccatcacgga aagaataata aagccgggaa
     7201 ttaatgccga gaaggaacgc gatactgaag caggtacgct gtcgggcagt ttaatgaccc
     7261 aattgcggcg gacaataaag gtaaacattt ctgccaccac caggccaata ataatcccgg
     7321 agatgatatt cgccccacct aaccagtttg cgccaaccgc ataggcctca ccgacactat
     7381 atggggtgac ggtcataaat gctgcaacgg ataacaaccc agccgccagc gcatcgactt
     7441 tacgctcttc tgccagcgcc atgccaataa agaacggtgc cattaaagac attatcccta
     7501 atgttccgtt atatacgttg ccgccaatac ctttcagatc attaagtgtt tcaatggttg
     7561 aggcgtcgag gcgaatacct aaggaataaa aaaacgaccc ctccccaaag cttagaaaaa
     7621 cgttgttaat taatacaaac atggcccccg caagggttaa cggcattaag cgaataaagc
     7681 catttttgat tgcattaacg tgtggctgct ttcctatttt aactgcaaaa gggaggagta
     7741 ccttttcaag cgatgcaata acattactca tagaaaaata cccttaaaaa cc
//
LOCUS       DAFNBJ010000202         7782 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000202 DAFNBJ010000000
VERSION     DAFNBJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.202"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            50..1204
                     /locus_tag="L2X49_004373"
     CDS             50..1204
                     /locus_tag="L2X49_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBN4799360.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKAITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            1355..2179
                     /locus_tag="L2X49_004374"
     CDS             1355..2179
                     /locus_tag="L2X49_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBN4799361.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINNSSNTEVIFWHSFPFAGNTKREYCGVILRQEKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAENLITGQILPVSLFNTSLLPW"
     gene            2265..3467
                     /locus_tag="L2X49_004375"
     CDS             2265..3467
                     /locus_tag="L2X49_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HBN4799362.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPNDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            3527..4111
                     /locus_tag="L2X49_004376"
     CDS             3527..4111
                     /locus_tag="L2X49_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBN4799363.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKDPVVMDSLSDCNNDLYKTLESIVRESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            4506..4964
                     /gene="traH"
                     /locus_tag="L2X49_004377"
     CDS             4506..4964
                     /gene="traH"
                     /locus_tag="L2X49_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HBN4799364.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            4961..5779
                     /gene="traI"
                     /locus_tag="L2X49_004378"
     CDS             4961..5779
                     /gene="traI"
                     /locus_tag="L2X49_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HBN4799365.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            5776..6924
                     /gene="traJ"
                     /locus_tag="L2X49_004379"
     CDS             5776..6924
                     /gene="traJ"
                     /locus_tag="L2X49_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HBN4799366.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVVPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAVSITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            6921..7211
                     /locus_tag="L2X49_004380"
     CDS             6921..7211
                     /locus_tag="L2X49_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBN4799367.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            7226..7777
                     /locus_tag="L2X49_004381"
     CDS             7226..7777
                     /locus_tag="L2X49_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBN4799368.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASVLVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWRP"
BASE COUNT         2116 a         1907 c         1801 g         1958 t
ORIGIN      
        1 cttgcagaga atgattgttg gcattatttt ttcctctttt gaggttagca tgccgtctcc
       61 acgcatccgt aaaatgtccc tgtcacgcgc actggataag tacctgaaaa cagtttctgt
      121 tcacaagaaa gggcatcaac aggagtttta ccggagcaat gttatcaagc gatatcccat
      181 tgcccttcgg aatatggacg aaataacaac cgttgatatt gctacataca gagacgttcg
      241 tttagcagaa atcaaccccc gaacgggtaa agccattaca ggtaatactg tacgtcttga
      301 actcgccctt ctgtcatctc tgttcaatat tgctcgtgtt gaatggggaa cctgtcgtac
      361 taacccggtt gaactggttc gcaagccgaa agtatcctcc ggacgagatc gtcggctaac
      421 gtcttcagaa gaacgtcgcc tttctcgcta tttccgcgaa aaaaatctga tgttgtatgt
      481 cattttccat cttgcccttg aaacagccat gcggcagggc gaaatactgg ccttacgttg
      541 ggaacacatt gatttgcgcc acggtgtggc tcatttacct gaaaccaaaa acggtcactc
      601 acgggatgtt cctctgtcca gacgtgcccg taactttctt cagatgatgc ccgttaatct
      661 ccacggcaat gtttttgatt acaccgcatc cggctttaaa aatgcctgga gaatagccac
      721 acaacgactt cgcatcgagg acctgcattt tcacgatcta cggcatgaag caataagccg
      781 cttcttcgaa ctgggtagcc tgaatgtaat ggagattgct gcaatatcag gacatcgttc
      841 catgaatatg ctgaaacggt atactcatct tcgtgcatgg caactggtca gtaagcttga
      901 tgcccgccgg cggcagacac aaaaagtggc agcatggttt gtgccgtatc ctgcccatat
      961 cacgaccatc gatgaagaaa atgggcagaa agcgcatcgt attgagatcg gtgattttga
     1021 taaccttcac gtcactgcca caacgaaaga ggaagcagtt caccgcgcca gtgaggtttt
     1081 gttgcgtaca ctggccattg cagcacagaa aggcgaacgt gtcccatctc ccggagcgtt
     1141 acctgttaac gaccctgact acattatgat ttgccctctg aacccgggca gcaccccgct
     1201 gtaaacatca ttcaaaacac atacagaaag gcgagttgca acaactcgcc aaacacattc
     1261 gatctgcatg atcgaaatta ttcgtttcac cagaaaaaac accgaagata caataaccac
     1321 atgagctgaa aagacttttt cagtcggagg aacaatgaga ctgaatacga ccggtattgc
     1381 ggcccgaatg atgctctcgc tggacaaaaa agcaatcggc gaaaaactca tcaacggtcg
     1441 tcagataaac ccgacacctc tgcaggtccg ttacccacaa ggggtaagag aagttctggg
     1501 gataatgagc gaacaattag ccatatccac cgctgattta acccgaatcc tgcttgagga
     1561 tgctcttcat aatatgttta tgcctgcgga taacaccaca ggaaacataa taagcagaat
     1621 tgaatacatc atgctgtcgc atgatataaa tgcgccatta ctggcaacat tgctgtcgcc
     1681 atggaatatc cgctccacgg ttatacagga cccggccaga cttgcagact atctttcagc
     1741 tgacgctctc gaacatcttg ctgaatgttt taatctcaat ccggactggc tgaatggcca
     1801 tgaaaactat ccgatagcgt tgtccggtga atggccagat actgctgata atttcagaat
     1861 gcttattaat aatagcagca acactgaagt tattttctgg catagtttcc ccttcgcagg
     1921 aaacacgaaa agagaatatt gcggggtaat attgagacag gaaaaagaaa taaatggctc
     1981 tgtaatatat ccggcactat cattatcacc aacacttctg aacgatgaaa aaagaaaatg
     2041 gttaactgaa tatacaacca ggcagaatac aacaatgtct ttgcgacgcg tcacgttgag
     2101 acctggcctt gccgagaacc ttattacagg gcaaatacta cctgttagct tatttaatac
     2161 gtctttatta ccctggtaaa taccagatag taccaaagta aaaaattacg gcacatccgt
     2221 gtcatagcgt tgttttgtct aaattctgat aaacaggaaa ttaaatgatg aagaaaaatc
     2281 atattacccg caccatcatt gcatctgctg tattattttc ctttaacgct gcagcagcga
     2341 cgtcatattt tgaagcgcgt aacgatgcaa tgggtggcac cggggtagca tcttcccatt
     2401 atggtgtggc acctctggca aacccggctt tactgacaaa gcacaacagc aatgatgatt
     2461 tcagccttct tctgccgtct gttggggcac aagttgctga tcctaatgat gtatcaaata
     2521 aagctgatga tgttaaagat gactgggatt tgtttgacag tgctgtagat aaccagcatg
     2581 gcgtacaaca ggcagcggca aatcttaaac atcgtctgca ggaattccgc aatatcaatg
     2641 cagatgctca ggtaggtgta tcagctgtag cagccatggc aaatgacact ctgccgtttg
     2701 cccttatggt caagtcttac ggaactgtca gcgtaaacgg aaaagtaaac gatgcagacc
     2761 tggattatct ggacaaagtt gccaatggaa caattaccga cgtcgataag aatgccctga
     2821 cctcacgcgc attcgggcgt gcagctgtaa ttaccgacgt cggaatttca tttgccaaag
     2881 aactggaaac ggccggacaa aaatggtctc ttggcgtcac accaaaatat cagcgtgtgg
     2941 acctgtttaa ctacaacgta acggttcgtg attacgacaa agacgatttt gacggtgaca
     3001 agtaccacaa cacgaaaaac ggattcaacg ccgatattgg tgcttataca gacctgaatg
     3061 acaactggac tgtcggtctt gttgcccaga acatcattcc acgcagtatt gatactaaag
     3121 tcgtcaatgg ctttaaagaa accttcaaag ttcgtccaca ggcaaccgcc ggtgtatcct
     3181 ggcataatga cctgtttacc actgctcttg acgttgatct gactcctgcc agtggcttca
     3241 cttctgacag caaacgtcag tttgcaagtg ttggtgctga atttaacgca tggaaatggg
     3301 cgcaattgcg tgccggttat cgtcagaaca tggcatcaaa ctccggaagc gccttcaccg
     3361 ctggcgttgg tatctctcca tttgacgtcg tacatatcga tgtttccggc ctggtaggaa
     3421 ctgaccacga ttacggtgca atggcacaac ttcagttcac tttctgatta ctgtccccgc
     3481 ccccggcact aaacccgggg gtatttttat attcatttta aataacatga tgaaaccacg
     3541 ttcttcttat tcaaagacag cattcattct tttattttct gttttcctgg tagctgcagt
     3601 gacaaaagca aaaagctcgc ttcccgatat caccctagaa caggcaaaag aaattaatgc
     3661 tgataacacc gttattttcc ttttccgcca tggagaacgt tgcgaccgtt cagatatgcc
     3721 atgctattcg gataaaagcg gaatcactat taccggtaca gagaaagcac agcaggaagg
     3781 tattaagttc gcaacaatct tcagtgaata tgatatttac tccagcaatg cggtaagaac
     3841 catacaaact gcaaaattct tctccggtaa agaccccgtc gttatggaca gtttatcaga
     3901 ctgcaataat gatctttata agacgcttga atcaatcgta agagaatctc ataaaaggaa
     3961 catagtaata atgacacata atcattgttt atcatttctg gccagagata ggctcggcaa
     4021 gaaattcaaa ccggcgtacc ttgatgccct gatcatgcat tacgatggta cccggctcat
     4081 acttgacgga aaatacaaca aagaggcata gaggatattt gaatttacat aaattctgat
     4141 atactcagta agatacaaga gtgtacgggc acatgtgcct gtctgaattg caggtcatcc
     4201 ggacgagggc cgggaactgt ttcagggaca agcgccggta cgttcgattc ataaatcgtc
     4261 gagtcatcca tcatggatcg attcggcgcg gagcgttaaa gattacgctc atattcgttt
     4321 tatgtctgac gacacatcaa ccagccagta tcaggaataa tcaccttcag taaactatga
     4381 ggctatttac ctggccacac tggcatttat ttccggtaac gcaagtatag ccggaaacaa
     4441 acatcatcct gtctgtaccg gagcaccggt atatcccatt ctgatttaac tgctcaggaa
     4501 ttattatgaa ccgtattata cctgcggggt gtcttgccgc cctttttctg gctggctgca
     4561 actccccttc tcagcatact cctgttccgc gtaactacgg tgctgcctct gatgtggcgg
     4621 ttacccggca cactcagtac acattatggg acaaaggtgt gttttccggc caacttccgg
     4681 tttcaacggg gtctcatctt ctgacagcta acagcgatcg cctttccttt gactgggaag
     4741 gtgatgcaat tgaattactg aacgaactgg ccagagtcag ggggatgcag tttaactaca
     4801 atggtgtccg cctgccgtta ccagtcaacc ttcacgtccg ggatatgacg ttttccaata
     4861 cgttgagact tattgaagcc cagacagcct ggagagcaac aatccatcaa tatccgggct
     4921 tgttacaggt cagcttcatg cagccggaga accggaaaaa atgaaagcac tcacctctct
     4981 cattttgcca gtaatactgc ttactggttg ttcttcttcc cctaccatct cgccaggtgg
     5041 tgtcaatacg ccaccacctg acattaatgc atggcttaaa ccacgaagtg aaaagccgga
     5101 tagtctgtca gaaacacgct ggaaaatgct gaccgacgca ggtaaaacgc ttggatttcg
     5161 tggtggcaag gctcagcgtt catgggagct gattcaggca ctcaacgccc gggaaagcac
     5221 acttaatgcc ttatacgatt tccggccact gattagcccg gaaggctggc taccaccggt
     5281 tattgatgaa gcacaggacg tagctcatat cacgccagat caaatccgga cctcttcccg
     5341 ggtctggacc atcattcgcc ctgaacgctt tgtcagcaat ccaccgggct ggcgtgactg
     5401 gcttctccgg gggctttcaa caacagccac tccgggtaca gaaggcagcg tcgttccaga
     5461 agacagcgtg caacgaaaag tgtgggaaac cgcattacgg cagggatggc aggaaggaag
     5521 acaaaacgcc gacctgacgc ttgaagccaa ccagaaaacc ctcacccgtg attaccgggg
     5581 catgatgctg tactcacttc tctggcgtca gggcatgatc acccgtccgg atgtttctga
     5641 tcaaatgcaa accgttacag gtgacgggaa aaaactggtc actggagatc gtgttcgtcg
     5701 ccttaaaaat catgcggaat tcaatcttca gaaatctcac tggcgtccgc ttataggcac
     5761 tgaaggaggc tcccgatgaa tattcctgat gagtttggtc tttttccgtt tcagcgtttc
     5821 actgcagatg aattacggca tttctttgtc tggtgtgccg cacataaagt cagtgatgtg
     5881 gatctgaccg gtgggtcacc tgtatctgtc agtcgctttg gccgacgggt gcgttgttcc
     5941 agtgcaacgt taccaaccac actcatgtcc tccctgattg atgaactgtt cggtcgggaa
     6001 gtcgttcccc gcgttctggc aggcaatccg gttgacagga cgatacagat taacggggat
     6061 gcatccggtc gctatggtct taaacgcgga gaacgcatcc ggttacgctc ccacctcatt
     6121 cagggcacat ctggtgcaga ggagaaagca gtctcaataa ccatgcgtgt catcccgaca
     6181 gaaataccgg acattctctc catgaacatt gaaccagacc tgcttgaagc aatggtctgc
     6241 aaaagcggtc tgggatttgt atgtggtgaa accgggtcag gaaaatcaac gctgtgttca
     6301 gcactgtacc gttacattat ggacaatttc cccgacgcca aaatcgtgac atatgaagac
     6361 ccggtggaat atatcctggg aaatgaaaac gatctgctcc caccgcatca ggcagaaatc
     6421 gggcgtgatg tggtcagttt tgctgccgga ctgcgttctg ctgtacgacg taatcccgaa
     6481 attatcggtg ttggtgagat cagggataac gaaaccgccg acgcagcggt acaggcaggc
     6541 aacaccgggc attactgcct gagcaccatg catacgaaat ctcctggtga aacgctggca
     6601 cgtcttcttg ggctgtttcc ccctgtcatc agggattcga tggcctgggc tgtcctgtca
     6661 ctgttgcagt tcattctggt tcaggttctg gtcagaacaa atgacggtgg tcgtaaagcc
     6721 gtccgggaat atatcgtcat taacgatgag cttcgcgaca atctttccgg aatgccgcat
     6781 gcagagtggg ggcatcacat tgatgccata atccggcagg agaagcgccg tatccgggac
     6841 cagattctgg aaatgtatat ccggaatgaa gttgaccgaa gagaagcgat tctttttatc
     6901 ccgccagggg agttacggtc atgaaaagag aaggtttctg gcgatatgca acaccgccag
     6961 tcaacatcca gggcgtacct ctgccgttca ccatgattta tctgtttata tgcccctttc
     7021 cctccaaaac aaccttctgg atttgcaccg gcatcatttt atttttcgtc attctcgacc
     7081 gttcaggctg gactgtcagg acgatctgta ctcgcatttt cagcattctg agaggggcca
     7141 tcgcctccgg tcgtccatgg tggtatcgcc accatacgga atcaccacga gactggactg
     7201 gcctcgatta aattctggag tctttatgtc aggtaaaaaa actatcgtta ctctgctccg
     7261 ggtatcgcta ctggcatgcc cgttactgtt cacgacacca tcgtttgcaa tgatcgacac
     7321 gccatccgtg aaggtcggct tttctcctga aggaagcgca tccgttctgg tgcttgacac
     7381 aatcaacagt gcggaaagct ccatcaggat gatggcatat tccttcaccg acccggatgt
     7441 aatgcatgcg ctggcaaaag cgaaaaaacg cggagtggac gtccgtattg ttgttgatga
     7501 caaggggaat acgaaccggg caagtcagga agcgatgaaa tatatcaacc tgctggacat
     7561 tcctctccgg actgtagatg ccttccccat ccatcacgac aaagtcatca ttgttgacgg
     7621 aaacacggtt gaaactggct cctataactt ctctcgtgct gctgcacgca agaattcaga
     7681 gaatgtcgtt gtgctcaaga atatgccgga tgtagccgca caatatcttg aacactggca
     7741 ggatcgctgg aataagggta cagactggag accctgacaa ac
//
LOCUS       DAFNBJ010000203         7752 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000203 DAFNBJ010000000
VERSION     DAFNBJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.203"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(103..2649)
                     /locus_tag="L2X49_004382"
                     /pseudo
     CDS             complement(103..2649)
                     /locus_tag="L2X49_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            2648..4510
                     /gene="tssM"
                     /locus_tag="L2X49_004383"
                     /pseudo
     CDS             2648..4510
                     /gene="tssM"
                     /locus_tag="L2X49_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            4521..5873
                     /locus_tag="L2X49_004384"
     CDS             4521..5873
                     /locus_tag="L2X49_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HBN4799369.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDTLEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            5897..6379
                     /locus_tag="L2X49_004385"
     CDS             5897..6379
                     /locus_tag="L2X49_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBN4799370.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            6423..7337
                     /locus_tag="L2X49_004386"
     CDS             6423..7337
                     /locus_tag="L2X49_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799371.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            7347..>7752
                     /locus_tag="L2X49_004387"
     CDS             7347..>7752
                     /locus_tag="L2X49_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799372.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFTSFSFLNIHVILLTIIKSFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPG"
BASE COUNT         1827 a         2146 c         1953 g         1826 t
ORIGIN      
        1 agagcttgat aaaaaatcta tagtttcttt catttgtgaa tcttgtttta aatactcaac
       61 agcctgatta aaatcacttg gatcaccgtt aacatggaat tttaaaccca aagaatctat
      121 aaaccgaaca gggtttaatg gatatttata cagattccat tcacctctca gccctatcgg
      181 gtcctgggtg atatatctcc cctgcaacgg atcatagtac cggttgcggt tgtagtacag
      241 ccccgactcc tcatcatact gctgtcccgg cagacggagt gactgctgaa ggtgctgcgc
      301 gctttcctcg cccagcaggt ttccccactc gtcatactcc ccctgccacg ccgtttcccc
      361 ttccgggctg ataagcgcca gcggcagccc acggtggtca cagtggtaaa gatgaagttt
      421 cctctccggg atgtattccg cctccagttg cgcggccatc tgttccgccg tcagcccgca
      481 ctgcgcaagc cactgctggc tttcttcact cacgctgccc tgccgcagtt cccgctccag
      541 cctccccagc atcaccgcca gctccgccgg tagcgtcaca cccgtgtcct cctgcaacac
      601 ctccgccagg ctacggtgcc gcgccttcgc ctgttcacca ttctctgttt cgattctcag
      661 gagcggcgtg aagcttcccg gctggtatac cgtctggatt cttgtctgtt gggtctgtac
      721 agtggtcagc ctgtcaccgt cccacccgta ccaggtctcc tccggtttac gcgacagcga
      781 catccacccc gtcaggtcac gctcccgccg ccacacccgt tttcccgtcc ggcggcccag
      841 cgggtcatag aggtagcggc tctccacctg cggctcaccg tgctgtatcc gcgtgtagaa
      901 caccagacgg tgctggctgt cgtagtggta gtggtgggtc cgctcgtcgt gcatccgtat
      961 caccccttcc gggatgcggt ccgtcttctc cgccagcctg ccgtattcat cgtagcggta
     1021 gacatagtgc gcatcttccg cgatgcggtt atccggccac gccgtgaggg tgctgtccgg
     1081 gtgcagttcc gggtccggca gccggtttcc tgccgggtcc gtcgcatacg ggatatcgat
     1141 atccaggttc gctgccgtag tgtggacgcc cgtcagcctt cccgtgccgc tgtaacggta
     1201 ctcccggcac tcctgcgggc cgctgatgcg caccagttgt ccgttatcgt tccacgtata
     1261 gtcacagtca agctgcggaa ggttcaggtg cctgctctgt agctgccctg tgagggtgta
     1321 cgccgtggcc tgttcgtacc ccgctgtact gcccgccccg ccgaagctac gggccgtctc
     1381 gcggtgcagc cggtcccgcg tgtactcgac cagcggcgtt ccgcccagct tcatccccgc
     1441 aagataaccg ctgccatacg tcagccactc caccggcggc agaccgtccg gctcctgacg
     1501 ggtcgccagc ccctgttcgc tgtacgcatg ccccgtctca tgctcccaca gcatctcccc
     1561 tgtctccggg ttctccaccg tctgccgttc acccgtcagg cggcctttat cgtcatagcc
     1621 gtagtggacc gacacgcggt ggccttcaca ggtatggctg agggtggtta accacccgtg
     1681 ctcatcatac tgccactgct ctgccgggtc gccgttcacc gtccggtgcg tgatgcggtc
     1741 cgacgcatcg tagtgccaga gggtgataag cccctcgtct tcactctgcg tgagttttcc
     1801 ggtcaggtca tagtggtaac gttgcgtccg gccgtcaaaa ccgcgctgtt cagtcagcct
     1861 gtccaccggg tcataccgga acgtggactg gctgccgttc tcgttggtca gcacggtgat
     1921 gcgcccggca gcgtcatacc ccatgctgcg cgtaagaccg ccttccgtca cactcaccgg
     1981 acgcccgcgt ttatcatatt cgatggtgct gcgtttaccg tccggtgata tcgttgctgt
     2041 caggtcgcct gcggcgctgt actcataacg gatttcacgc ccctgcgcat ccttctgact
     2101 gaccagttgc ccacgcgggt tataactgct gtaagtgctg atgccttcct cccggtgaac
     2161 ggcgatttgt tgaccgtacc ggtcgtactc ataccgcgtc gtgtaccccg agcagtccgt
     2221 gaaggccagc agttgaccgt aacggctcca tgccatctgt tttgtactgc ccgtcgcgtc
     2281 ctgtatccct gtcggcagct cactcgccgg gtcatcatag ctgtaccgcg ttgtctcacc
     2341 gctgcgcgag gtctccgccg tcagccttcc cttctcatca tactcccggc tgctgcgaag
     2401 cccgtccggg tacgtcaccg acgtcacctg ccgctggctg ttatagccat accgtaccgt
     2461 cctgccgtcc ggccccgtca ccgccgtcac cgcacccgac gccatatgca ggctgtactc
     2521 cgtccgccgc cccgccgcat ccgtctgtgc cttcagcctc cccgcctcat catactcgct
     2581 gcgggtaatg ctcccgtccg catgttcctt cttcaccacc cgcttcaggc caccctcgcc
     2641 ttccgtgtac ctgccctcgc tgtttaacgg gctggtcaaa gcgatgaacg ccgcgccgcc
     2701 ggagagtgaa gaaaaactcg cggtgctgcg cgtgatgcga atgctggagg acaaaagcgg
     2761 acgtaacaat caggtggtga agcagtatat ggcaaaacgc tggagcgaaa agttccacgg
     2821 tcagcgcgat atccaggcac aactgatgtc ccatcttgac tacgcgctgg ctcatactga
     2881 ctggcacgca gagcgtcagg cgggcgacgg tgacgccatc agccgctgga cgccatatga
     2941 caagcccgtg gtatcagcac agaaagaact gagcaaactg cctgtctacc agcgggttta
     3001 ccagagcctg aaaacgcggg cgctgggcgt tcttcctgcc gacctcaatc tgcgtgacca
     3061 ggtagggcca acctttgacc aggtgtttac atctgccgat gacaacaaac tggttgttcc
     3121 acagtttctt acccgttacg gcctgcaaag ctattttgta aaacagcgcg atgaactggt
     3181 tgaactgacg gcgatggatt cctgggtact taaccttacc cgcagcgtga aatacagtga
     3241 cgccgaccgc gcggaaatcc agcgccagtt gaccgagcag tatatcagcg actacaccgc
     3301 cacctggcgg gccgggatgg acaatctgaa tatccgcaat tttgagtcca tcggacaact
     3361 gaccggggcg ctggagcagg ttatcagcgg cgaccagcct ttgcagcggg cgctgaccgt
     3421 gctgcgtgac aacacacagc caggcgtctt ttctgaaaaa ctctctgcca aagaacggga
     3481 ggaagccctg gcagagccgg attaccagtt actcacccgc ctcgggcatg aattcgcccc
     3541 ggaaaacagt accctggcag tacagaaaga caaagaaagc acgatgcagg ccgtgtatca
     3601 gcaactcacc gagttgcacc gctacctgct ggcaatccag aacgcgcctg taccagggaa
     3661 atcggcgctg aaagccgtgc agttacggct tgatcagaac agcagcgatc cgatattcgc
     3721 cacccgccag atggcaaaaa cgctgcctgc tccgctcaac cgctgggttg gcagactgac
     3781 tgaccaggcc tggcatgtgg tgatggtgga ggctgttcat tatatggaag tggactggcg
     3841 cgacagcgtg gtgaaaccgt ttaacgagca actggcaaat aactatccgt ttaatccgcg
     3901 ttctgcacag gatgcctcac tggatgcctt cgaacgcttc tttaaaccgg atggcatact
     3961 ggataccttc taccagcaga acctgaagct gtttatcgat aatgacctga gtctggagga
     4021 tggcgataac aacgtcatta ttcgcgaaga tattattgcg caactggaaa ctgcgcagaa
     4081 aatccgtgac atcttcttca gcaaacagaa cggtctggga gcatcctttg ccgtggaaac
     4141 ggtatcgctt tcaggcaata aacgccgcag tgtactgaac cttgacggtc agttagtcga
     4201 ttacagccag ggccgtaact ataccgccca tctggtctgg cctaacaaca tgcgcgaagg
     4261 caacgaaagt aagctgacgc tcatcggcac cagcggcaac gcgccgcgca gtatcagctt
     4321 cagcgggccg tgggcgcagt tccgcctgtt cggggccgga caactgaccg gagtacagga
     4381 tggcaacttt accgtgcgct ttagcgtgga cggtggcgcg atgacctacc gtgtgcatac
     4441 cgacacggaa gataacccgt tcagcggtgg gttgttcagc cagtttggtc tgtcagacac
     4501 actgtactga ggaggcaggg atgaatagta acgtactgac acaaactatc gttaccggca
     4561 gtgacccgcg cgggctgccg gaattcagcg ccatccgcga ggaaataaac aaagccagcc
     4621 acccgtcaca gcctgagctg aactggaaac tggtggagtc gctggcgctg gcgattttta
     4681 aagccaacgg tgtggattta cacaccgcca cctactatac gcttgcccgg acacggacac
     4741 agggactggc gggattctgc gaaggtgcgg aactgctggc ggcaatggta agccacgact
     4801 gggataagtt ctggccgcag ggcggcccgg cgcgtactga aatgctggac tggtttaact
     4861 cccgaaccgg caatattctg cgtcagcaaa tctcctttgc ggaatccgac ctaccactga
     4921 tctaccgcac agagcgggca ttgcagctta tctgcgataa gctccagcag gtggaactga
     4981 agcgcgttcc gcgcgtggag aatctgctct attttatgca gaacacgcgt aaacggcttg
     5041 aaccccagct gaagagtaac actgagaacg ccgcacagac cacggtcaga acgctgattt
     5101 atgccccgga aacacaggca tcttccacac cagaagcggt agtgcctccc ctgcccggcc
     5161 tgcctgagat gaaagtggaa gtgcgcagtc tgacagagaa tcccccacag gccagtgtga
     5221 taaagcaagg cagtacggta agagggttta tcgcagggat cgcctgttca gtggctgtcg
     5281 cctcagcatt gtggtggtgg caggtctatc cggtgcagca gcaactgtta caggttaacg
     5341 acaccgctca gggcgcagca acggtgtgga tggcctcacc tgaactcgaa aactatgagc
     5401 gcaggctgca acaacttctt gatacctccc cggtacagcc gctggaaacc gggatgcaga
     5461 tgatgcgtgt tgccgacagt cgctggccgg aaagcctgca acagcaacag gcctcgacac
     5521 aatggaatga ggcactcaaa acccgcgcac agagtagccc gcagttgcgt ggctggttgc
     5581 agacccgcca ggacttacat gcttttgcag atctagtgat gcagcgcgag aaagagggac
     5641 taaccctttc ctatatcaaa aatgtcatct ggcaggcgga gcggggactg gggcaggaaa
     5701 cacccgttga gtctctgttg acgcagtacc atgatgcccg tgcgcagaag cagaatacag
     5761 atacgctgga aaaacaaatt aatgagcgac tcgaaggcgt gttaagccgc tggctgctgc
     5821 tgaagaataa cgtcatgcca gaggcggcaa ccggtaccac ggccgaaaaa taattattta
     5881 attaaaggag attatcatgg caaatataag ttatttatct ttgagtggag agacacaagg
     5941 gctaatttca gcaggttgct caacgcttga ttctgtcgga aacaaagcgc agccagaaca
     6001 taaagatcag atcatggttt atgcgttaat gcattccatc tcaagaagtc aaaatgtcaa
     6061 tcatcatgaa ttaattatca ccaaaccggt tgataaatcc tcaccattgc tggcaaaagc
     6121 acttagtgac aatgaaaaaa tggcaatatg tgaatttata ctctaccgaa ccagtaaagc
     6181 gggtatttac cagccttatt ataaaataaa tctgtcaaaa gcccgaatat caagtattga
     6241 ttttgtcaca ccgcatgctg tcctggaaaa agaactagaa ccacaggaga gaattgcctt
     6301 tatttatgaa gatatttcct gggagcatac gttagccggt acgaatgcaa tgagtaaatg
     6361 gcaggatcgt gtacagtaac ttactgtaat aacataataa attactattg cggagagata
     6421 aaatggctgg tttaaggaat aacaacaaca cacagaatgc acagtgggct gattatgtgg
     6481 gtgacattct tcggggggct cagccgataa accagctagt acctcagcac ccttatctta
     6541 acgacgtccc actcattgat gagttaaggc atcagaacac tcatcatgtt attctcttga
     6601 cacttgatgt tgcaaaaaaa atactcagcc caataacatc atttgattac attcatttta
     6661 tcactacaca tccttcaggt ataaaagata cactcgcctg gttagttaac gcgagtaaat
     6721 taatgactga gtttgatgac aatgggaaaa ttatatttaa ccttaatgcc ttaaagtata
     6781 caaaagcaag ttactttgaa atacttggag agaaatatat aaaaatcaca acatccagcc
     6841 cctggctttt agaaaagctc ggtaaatata ttttctcttc acgagcgcct caagttctgg
     6901 aactcgctat tggctggcga ggagctttat ctgaatcaat aaaaggagtt aagttctgta
     6961 tctggttttc agtagcctgg agaaccatag aatttattat gagttcagag cgagatctgg
     7021 ttaattttct tggcgatttt tcaatggatg tggccaaagc agttatagcc ggtggtgttg
     7081 caaccgctat tggaagcctg gcttcttttg cctgtgttag ctttggcttt ccagtaattc
     7141 ttgtcggagg agcaatttta ctgacaggga tagtgtgtac agttgtttta aatgaaatcg
     7201 atgctcaatg ccatttatca gaaaaattaa aatatgcaat tagagatgga ctaaaacggc
     7261 aacaggaact tgataaatgg aaaagggaaa acatgactcc atttatgtat gttcttaaca
     7321 ctccacccgt gatatgagga ttaaccatgc gtttacatgt taaattaaaa gagtttcttt
     7381 cgatgttttt tatggccata ttatttttcc ctgcattcaa tgcttctttg ttttttaccg
     7441 gagttaaacc tctatattca ataataaaat gctccacgga aatattttat gattggagaa
     7501 tgttgattct gtgcttcggt ttcacgtcgt tttccttttt aaatattcat gtaattttac
     7561 ttacgataat aaaatcattc ttaatcaaaa aaacgaaggt agtcaatttc gccacagaca
     7621 taacaataca gctaactctt atatttttac taatagctat cgtaatagca cctttaatag
     7681 ctccttttgt cacaggatat gtcaatgcta attatcaccc ctgcggtaat aatacgggaa
     7741 ttttcccagg ag
//
LOCUS       DAFNBJ010000204         7642 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000204 DAFNBJ010000000
VERSION     DAFNBJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.204"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(84..260)
                     /locus_tag="L2X49_004388"
     CDS             complement(84..260)
                     /locus_tag="L2X49_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799373.1"
                     /translation="MSKSSPVLPEDRDDIEFVMDDFQRSLGKSKPVREVQFLGESQQS
                     EESANSADSPWFES"
     gene            complement(275..1951)
                     /locus_tag="L2X49_004389"
     CDS             complement(275..1951)
                     /locus_tag="L2X49_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBN4799374.1"
                     /translation="MSAVESDSLPMGAPGSSLVAYMPHTGLLINDDNFLPEMREELDV
                     INRQVESYNDELSLLRSMSAQFLKCGNQKLYSYLSGYNHLISEADASYAENALRSEYW
                     KRVMALTDVLSVMSDEKKKEWDKQFTADRYHLPPQEIPDFTLDAVVTTVVALLNDRHQ
                     FIKDRVYAVFQSLSRQHKTNKAFGFSTRMITSGVCEGVKDKWVKLKVEFKESGLMPLS
                     ELRVICSYFRGETVKPVYDTKKMVENMVGHVGFRNWISLDGNSIRFRVYKNGSMHIDV
                     HPDIAERLNNILAAIVPLALPAERVAHTKATLQEFPALKRCIDFHSRMQLAELDFTQN
                     EKEWSSWTSLGYAGENADKTRQVNADVLRFLGARVTSYHVTFDYDPTEVMRFIGHMGA
                     MPDIKSHQFYPSSARLSEYVASIIAAGEGERLLEPNIGHGDLLQCFPKSVNVTGIELD
                     TLNCLVSRAKGYETTEADFLAWSAANQIQKFDCVVMNPPFADNRAKLHLLAAASHLVP
                     GGRLAAVLPLSLQGIDNLLGEGFRTEWMDTFDNEFDNTAVSVRVLYAERF"
     gene            complement(2215..3372)
                     /locus_tag="L2X49_004390"
     CDS             complement(2215..3372)
                     /locus_tag="L2X49_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBN4799375.1"
                     /translation="MSDLMTDSQQQTNSRVFKSPLVSFSTVTGSGRFVSGMKQFRDAN
                     PDLCNEVTEQKAWAISPTVVQITEGFNSREMGMGEAYYQLPEVADHLNNIKNAYIRGD
                     YVDPIRVLIKDGIPYVRQGHCRLKAALMAVAEGHSNLTILCIEMKADEISCELTTIDG
                     NRGLALSPVALGESYRRLQALGGWSLDRIAEREGKSNTTIAALIRLTGCCVYLKKLIH
                     ADAISYVAVLSLIDEFGETEAVARIEKMIADLEKADSSGIQIKKTPRGQIRVVPSDFK
                     PARVPPVLATKAVEGVKLITTSLLQKLGDIELPEVTDSSEDEEITFTLNRSMLEMLKN
                     LQSEITESENKQLRRAENRQARLKGEKVTYKTKKNDKNGGEPQSETPPAQA"
     gene            3732..4556
                     /locus_tag="L2X49_004391"
     CDS             3732..4556
                     /locus_tag="L2X49_004391"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HBN4799376.1"
                     /translation="MRKHTPIIKWAGGKTKLMPFLSKHFPHDKSRRWVEPFIGGGAVF
                     LNMFATEALLADSNPDLINLYRNIQRNKPAFIREVQLLAERHFEEEDYYVLRNTFNST
                     SFDDAPLQRAAIFYAMNRLGYNGLCRYNLKRKFSVPWGKRYQFSLDIQKVDYLSFRLS
                     SVELKTADFGQTLEFAGGGDQIYCDPPYDKISKTSFVSYDGIPFDKSAHVKLADMLVD
                     ANRKGASVAISNSMTPFTLELYEERGFDIHTHNAYRSVGSQSKSRKKEIEILAVLR"
     gene            complement(4571..5392)
                     /locus_tag="L2X49_004392"
     CDS             complement(4571..5392)
                     /locus_tag="L2X49_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799377.1"
                     /translation="MQNDLGLGFIRKSARTSAKIISRKVTFPLDDLDNAKLTLTGLQC
                     HPSCRFPFYQLSMLIHNQLAEHGVISADENAPFTVSISIHEWLSRARVAFIDYHGLRN
                     SDKTVMITRAHLEELLTYLSTTLAVYVPYSKKRLNFSFLTSFTLVKTSQSYTLTFPGV
                     LRPFLMLLGGLIQEFMTEKLLKRRNAQFVIFDYLRKSRRHSHKITDIVNDLQLHTVNV
                     RIMNVLTQLADQGFISFICEGKRGERSIEELQFIPYASRDHSEVLSFEEWISPID"
     gene            complement(5667..6374)
                     /locus_tag="L2X49_004393"
     CDS             complement(5667..6374)
                     /locus_tag="L2X49_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799378.1"
                     /translation="MKHSLFFTQDGYLLPSSVKPIYGKDGHYYAVITRECFECGGTGK
                     IANAVCVTCNGSKTSPATEEKLVLKECIEFLETIPELRCNPDLEAWKRRHSEQLALLQ
                     ADNSKFAASIAQQLNNNLFLTVRQLESFYSTYQPVSEAFSENAGFPIEHSFNIGDVVS
                     FDIIFTEVRAAFSKGSHSYFFTFTGKKQNDGFALTGNVQDHVLLGHSYHLSGKVRKIH
                     YFDSVPYALLTVFKLTA"
     gene            complement(6371..6571)
                     /locus_tag="L2X49_004394"
     CDS             complement(6371..6571)
                     /locus_tag="L2X49_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799379.1"
                     /translation="MDKIAQFTVINTYNDLLITKGTLDRNIEDIKAGKRFDHFEEGHD
                     QYVQKVTDALQLVEARLENLKK"
     gene            complement(6589..7641)
                     /locus_tag="L2X49_004395"
     CDS             complement(6589..7641)
                     /locus_tag="L2X49_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799380.1"
                     /translation="MEISPKALFIESGFNPREAVIGELCYEQEPVKSTIVSIKQAYKE
                     GRRVDLIKVVKKDSNYVVRQGHCRYRALSLALAEGADIQLISVVVMNYKNKSEEYLEN
                     LDGNRNNGLNPVALAYALAEALKLGSTVESLAMRYQRSSTAIRNQLKILDMPLELQRL
                     ISLNLIKKTLAIEIMLKYSNDHAKVLAHLESCSLLVPSTEPEPVLSKTSSVQTSSNDE
                     KLDNPETLPVHVIPKEVKTKSITRSSLGIKRLSHKKTERLKTEFIGLIDTVRPALNDS
                     NKLTELIFDGQHSNLFTNISSTSITKTTESVKITLDEAKLQLLLSLLNDENTAIIIPK
                     SKVTELHEIRDLLTSE"
BASE COUNT         2106 a         1719 c         1739 g         2078 t
ORIGIN      
        1 gttcagtaca ggtaaaagtt gtaataactc cccgctcact gccggggagt cattttaaat
       61 cagcacggcg ccatcagaat agattagctt tcaaaccagg gcgaatcggc agagttcgca
      121 gattcttcac tttgctgtga ctctcccaga aattgcactt cacgtactgg tttacttttc
      181 cccaggctcc gctggaaatc atccatcaca aactcaatat catcacggtc ctcaggcaag
      241 acaggcgaac ttttggacat agtctttcct tcagttaaaa gcgttctgca tacagaacgc
      301 ggacggagac ggcagtgttg tcaaactcat tatcgaaggt atccatccac tcggtcctga
      361 acccctcgcc cagcaggtta tcgatcccct gaagggacag aggcaggacg gctgctaaac
      421 gcccgccggg aaccagatga gacgcagcgg ccagcagatg cagcttagcc cggttgtcag
      481 caaaaggagg gttcatcacc acgcaatcaa atttctgaat ctgattcgcc gccgaccagg
      541 ccagaaaatc ggcttccgtg gtctcgtaac ctttagcccg ggagaccaga caattcagtg
      601 tatccagttc gatgccggtt acgttgacac ttttcgggaa acattgaagc aaatccccgt
      661 gcccaatatt gggctccagc agccgttccc cctccccggc cgcaataatg gatgcgacgt
      721 attcactgag tcgcgccgac gacggataaa actggtggga tttaatatca ggcatggcgc
      781 ccatatgtcc gatgaagcgc atcacttcgg tcgggtcata atcaaacgtc acatggtagc
      841 ttgtcactcg ggcaccgaga aatcgcagaa catccgcgtt cacctgccgc gttttatcag
      901 cgttctcacc ggcatagccg agcgatgtcc agctggacca ttccttttca ttctgggtaa
      961 aatccagctc tgcaagctgc atccgtgaat gaaaatcgat gcagcgtttc agtgccggga
     1021 actcctgcaa cgtcgctttg gtatgggcca cgcgttcagc aggcagagcc agagggacga
     1081 tggcagcaag aatgttgtta agccgttcgg cgatatcagg atgcacatca atgtgcatcg
     1141 atccgttttt gtagacccga aaacgaatac tgttgccatc caggctgatc cagtttctga
     1201 atccaacatg accaaccata ttctcaacca tttttttcgt gtcatagacc ggtttaactg
     1261 tttcgccccg gaaataagaa cagatcaccc gcaactccga aagtggcatg aggccggatt
     1321 ctttaaactc aaccttcagt tttacccatt tgtctttcac gccttcacat acgccggagg
     1381 tgatcatgcg agtagagaag ccaaatgctt tattggtttt gtgttgacgg cttaatgact
     1441 gaaagacggc atagacacga tctttaatga actgatgccg gtcattgagc aacgcgacaa
     1501 ccgtggtaac aacagcatcg agtgtaaaat cagggatctc ctgaggagga aggtggtaac
     1561 ggtcagcagt aaactgctta tcccattctt tcttcttttc gtcagacatc acagacagta
     1621 catcggtcag ggccatgacc ctcttccagt attctgaacg cagcgcgttt tctgcgtagc
     1681 tggcatccgc ttctgaaatg agatgattgt atccggagag gtatgaatag agcttctgat
     1741 tcccgcattt caggaactga gcggacatgg acctcagtaa gctaagctca tcgttatatg
     1801 attcgacctg cctgttaatg acatcgagct cttcccgcat ttcgggcagg aagttatcgt
     1861 cgttaataag gaggccagta tgaggcatat aagcaaccag agaactccca ggggcaccca
     1921 ttggcaggga atcagattca acagcagaca tagcaccagt cctttttgaa gggtttatct
     1981 gataccagga taacaacctt gaacaaaaag ctaataggtt ggctgacatg aaaatggaga
     2041 aattaacact gcggaaagtc agaaagacag atgtggtttt aatcagaaga aacgggccgg
     2101 agacaggaca aaaaaacccc tacatagtag gggcaacggg gggagagcct gggtggctcg
     2161 ctctaaaata tcgtacttct tattgtagtc ttctcacact ctgaaataat tcatttaagc
     2221 ctgtgctggg ggtgtttcgg attgcggttc tccgccattt ttatcgtttt tcttggtttt
     2281 gtaagtcact ttttcccctt tcaatcgggc ttgccgattt tcggcccggc gaagctgctt
     2341 gttttctgat tccgtaatct cactttgcag gttcttgagc atctccagca tgctccggtt
     2401 cagcgtaaag gtaatttctt catcttcgct ggagtcggtc acctccggca actcgatatc
     2461 accgagtttc tgcaatagac tcgtcgtaat gagtttgacc ccttcgacgg cttttgtagc
     2521 gagaactggt ggaacacgtg cgggtttaaa atcggaaggg acaacacgga tctgcccgcg
     2581 tggcgttttc ttaatctgga taccgcttga atcagccttt tcaaggtctg cgatcatctt
     2641 ttcgatccgc gcaacagctt ccgtttcacc gaactcatcg ataagggaca ggactgctac
     2701 ataggaaatg gcatcggcat ggatcagttt tttgagatag acacagcagc cggttaaacg
     2761 aataagagca gcaattgtgg tattactttt tccctcacgt tcggcaatac ggtcaagcga
     2821 ccatccaccc agcgcctgaa gacgtcggta ggattcaccc aaagccacag gagaaagagc
     2881 caggccacgg tttccgtcaa tcgttgtcaa ttcgcagctg atctcatcgg ctttcatctc
     2941 gatgcaaaga atggtgaggt tagaatgccc ttctgccaca gccatcaaag cagcctttaa
     3001 acggcaatgt ccctggcgaa cgtacggaat gccgtctttg atcagaacgc ggatagggtc
     3061 tacgtaatca ccccggatgt acgcattctt gatgttattg agatgatcgg cgacttctgg
     3121 caactggtaa taagcttccc ccattcccat ctcacgggag ttgaatcctt ccgtgatttg
     3181 aaccacggtt gggctgatag cccatgcttt ctgttcagtg acttcattac aaaggtcagg
     3241 gttcgcgtcg cggaactgct tcataccact gacgaatcgg ccggaaccgg tcactgttga
     3301 aaaagacacg agtggggatt taaagacccg tgagttagtt tgctgttggg aatcagtcat
     3361 cagatcactc atagatactc cgttgaattc ttttttgtca tacctttcat ctgtgcgtga
     3421 atactaccgg aaaaaaaaat ctcacttttt cgttaaaaag gtactttttg ggtctattcc
     3481 gaattgaacg aggaaaattg ttctggcgat cagaacaaaa agaaaaaata actcaaataa
     3541 aacagatggt tatggttaat tcgtaaagga taaacttttt tctgagaagt gaaaaaaaat
     3601 taaaaaatgt gtcgaaatga caatctcgtt aacaaaagcc acgttttttc ctgctcatac
     3661 cttattggta gcaatccgcc tttcggtacc ataagtgtgg cataccgata aagctgaacg
     3721 gagaagactc gttgcgaaaa catacaccaa tcatcaaatg ggcggggggt aaaaccaaac
     3781 tgatgccgtt cctcagtaag cacttcccac atgataagag ccgacgctgg gttgaaccgt
     3841 ttatcggcgg tggcgccgtg tttctgaaca tgtttgccac cgaggctctc ctagcagaca
     3901 gcaacccgga cctaatcaat ctgtaccgca acatacagcg aaataaaccg gcattcatca
     3961 gagaagtgca gctgctggcg gaaaggcatt ttgaagaaga ggattactac gtgctgcgta
     4021 atacctttaa cagcaccagt tttgatgatg ccccgctaca acgagcagcc attttctacg
     4081 cgatgaatcg actcggatat aacggtctgt gtcgttacaa cctgaagcga aagttctcag
     4141 ttccatgggg aaagcgatac cagttcagtc tggatatcca gaaggttgat tacctctcat
     4201 tcaggctttc gagcgttgaa ttgaaaaccg cagattttgg tcagacgctg gagtttgcag
     4261 gtggtggtga ccaaatatat tgcgatccgc catatgacaa aatcagtaaa accagctttg
     4321 taagctatga tggtataccg ttcgataaga gcgcacatgt taaactggcg gatatgctgg
     4381 tggatgctaa tcgtaaaggg gcctcagtgg ccatatccaa cagcatgact ccctttacgc
     4441 tggagctcta tgaagaacgc ggttttgata ttcatactca caacgcttat cggtctgtcg
     4501 gcagtcaatc aaagtcgcgt aagaaggaaa tagaaattct ggccgtcctt cgttgagcgg
     4561 cgatcagtag ttagtcgatc gggcttatcc attcctcgaa gctcagcact tcggaatggt
     4621 cacggctggc atagggaata aactgaagct cttcaattga tcgctcccct ctcttacctt
     4681 cgcatataaa cgatatgaac ccctgatctg caagttgggt aaggacattc atgattcgta
     4741 cgttcactgt atgcaactga agatcattca cgatatcggt aattttgtgt gaatggcgcc
     4801 gggactttcg caaataatca aagatgacaa actgggcatt ccgtcgcttc aataattttt
     4861 ccgtcataaa ttcctgaatt aatcccccca gcaacatcag gaacggacga agtaccccag
     4921 ggaacgttaa cgtataagac tgcgatgttt tgaccagagt gaaggacgtc aggaagctga
     4981 agtttagtcg cttctttgaa tacgggacat aaacagccaa tgtcgtagac aagtaagtaa
     5041 gcaattcttc cagatgagcg cgtgtgatca tgaccgtctt atcgctatta cgaagaccgt
     5101 gataatcaat aaatgccact ctggcacgac tcagccactc gtgaatgctt atggatacag
     5161 tgaacggtgc gttttcatcg gcagaaatga cgccgtgctc agcgagttgg ttatggataa
     5221 gcatagacag ctggtaaaat ggaaaacggc aggatggatg acactgtagg ccagttagcg
     5281 ttagctttgc attatccaag tcatcgagcg gaaaagtaac ttttcgggaa ataatttttg
     5341 ctgacgttct tgctgacttg cggatgaacc caagtccaag atcattttgc atatatcact
     5401 ccgttttaac aaaatctcct taacctatat gttaagaaga gacaaaaaat ataattaata
     5461 agattatcat ttttaattta aaagattagt taataacggg tgttgaattt aaaataaatc
     5521 ggcttatttt cggagtgtaa aaccgggtaa aaacccgatg cgtgagcaac agatttgacg
     5581 tgagtgagtc ggcacgacag tgcagaaagc cggtggctaa tgtaaaaacg gcctgtattt
     5641 gggagttccg gtcactagac accaggctat gctgttaatt taaacacagt cagtaatgcg
     5701 tagggcactg agtcaaagta gtggatcttt ctcacttttc ccgaaagatg atagctatgc
     5761 cccaataaaa cgtgatcctg aacattacca gtcaacgcga atccatcatt ctgctttttg
     5821 cctgtaaaag taaaaaaata gctatgagaa ccttttgaaa acgcagctcg cacttctgta
     5881 aaaataatat caaaactaac tacatcacca atgttgaaag aatgttcaat tggaaaaccg
     5941 gcgttttctg aaaaagcttc agaaacaggc tggtatgtcg agtagaaaga ctccaactga
     6001 cggactgtta aaaagagatt gttgtttaac tgctgtgcaa tgcttgctgc aaacttagaa
     6061 ttgtcagctt gcagcaatgc taactgctcg ctgtgcctac gtttccaggc ttcaagatcg
     6121 ggattgcagc gaagttccgg gatagtttca aggaattcaa tgcactcttt cagtaccagc
     6181 ttttcctctg tggctggaga cgttttggag ccattgcatg tcacacagac ggcgttcgca
     6241 atctttccag tccctccgca ttcaaagcac tcacgggtga taacggcgta atagtgtcca
     6301 tctttaccgt agataggctt tacggatgat ggcagtaaat aaccatcctg agtaaaaaaa
     6361 agagaatgtt tcatttttta aggttctcca gtcgagcctc cacgagctgt aaagcgtcag
     6421 ttaccttctg tacgtattga tcgtgccctt cttcaaaatg atcaaaacgc tttccagcct
     6481 tgatatcttc aatgttgcga tcgagtgtgc cttttgtaat aagtagatcg ttataagtat
     6541 taattacagt aaactgtgca atcttgtcca taaacggtcc ttagcattct actcacttgt
     6601 taacaggtca cgaatttcat gcagttctgt aaccttagat tttggaatga tgatagcagt
     6661 attttcatca ttcagaaggc ttaataaaag ctggagtttt gcttcatcta gagtgatctt
     6721 tacactttcc gttgtctttg tgatggaagt gctgctgatg ttcgtaaata aattggaatg
     6781 ttgtccgtcg aaaattaatt cggtgagttt attactgtcg tttagtgctg gccgtacagt
     6841 atcaatcagc ccgataaatt cagtttttaa gcgttcagtt tttttatgag aaagccgttt
     6901 aatacccaat gaactgcgag tgatggattt tgttttaact tccttaggaa taacatgaac
     6961 agggagtgtt tccgggttat ctagtttctc atcgtttgat gatgtttgaa cgcttgatgt
     7021 cttagataaa acaggttctg gttcagttga tggtactaaa agagaacacg attccaaatg
     7081 tgcaaggact tttgcatggt cattggaata cttcaacatg atctcaatcg cgagtgtttt
     7141 tttaatcagg tttagactga ttaaacgttg tagttctaaa ggcatatcaa ggattttaag
     7201 ctggttacga atagcagtgc tagaccgttg atagcgcata gcaagggatt caacggtaga
     7261 gccaagcttc agcgcctctg ccagtgcata agctaaggct acgggattaa gaccgttatt
     7321 cctattccca tcaaggttct ccaggtattc ttcactttta ttcttatagt tcataacaac
     7381 tactgaaatg agctgaatat cagctccttc cgcaagagca agagatagtg ctctgtaacg
     7441 gcaatgcccc tgccttacaa cgtaattgga gtctttctta acaaccttaa ttaaatcgac
     7501 tcgtctgccc tctttatagg cttgtttaat agaaacaatg gtcgatttta ctggttcctg
     7561 ttcataacaa agttcaccaa ttaccgcttc acgtggattg aatccagatt cgataaaaag
     7621 tgctttagga ctgatctcaa ta
//
LOCUS       DAFNBJ010000205         7451 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000205 DAFNBJ010000000
VERSION     DAFNBJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.205"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..499
                     /locus_tag="L2X49_004396"
     CDS             <1..499
                     /locus_tag="L2X49_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBN4799381.1"
                     /translation="PEEVIRYINENITADFESGTLFRNGVKIEGSLVNPKPRQYLAFG
                     CRINGKFRILKVHQVIFYLYHGKQAKLWIDHIDGDPLNNAISNLREVTKEQNQANMIP
                     RKKTKSGYKGVHPRVDNKGFWATIRCNGKSHYLGQFNTAEEAAKAYDAASLELHGKYG
                     HRNFS"
     gene            1534..1881
                     /locus_tag="L2X49_004397"
     CDS             1534..1881
                     /locus_tag="L2X49_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBN4799382.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            1934..2329
                     /locus_tag="L2X49_004398"
     CDS             1934..2329
                     /locus_tag="L2X49_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBN4799383.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIALMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2370..3113
                     /gene="cmoA"
                     /locus_tag="L2X49_004399"
     CDS             2370..3113
                     /gene="cmoA"
                     /locus_tag="L2X49_004399"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBN4799384.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3110..4081
                     /gene="cmoB"
                     /locus_tag="L2X49_004400"
     CDS             3110..4081
                     /gene="cmoB"
                     /locus_tag="L2X49_004400"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBN4799385.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4246..6675)
                     /gene="torZ"
                     /locus_tag="L2X49_004401"
     CDS             complement(4246..6675)
                     /gene="torZ"
                     /locus_tag="L2X49_004401"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBN4799386.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAGLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6700..>7451)
                     /locus_tag="L2X49_004402"
     CDS             complement(6700..>7451)
                     /locus_tag="L2X49_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBN4799387.1"
                     /translation="WKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGETC
                     IDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGADL
                     TVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEY
                     GNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIAK
                     GMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1823 a         1846 c         1715 g         2067 t
ORIGIN      
        1 gcctgaagaa gttataagat acatcaatga aaatattaca gcagattttg aatctggaac
       61 attatttaga aatggtgtaa aaatcgaggg ttcacttgta aatcctaaac caagacaata
      121 cctggctttt ggttgtcgga ttaatggtaa gttcagaata cttaaggtgc atcaggttat
      181 tttctatcta tatcatggta aacaagctaa actatggatt gaccatatag atggtgaccc
      241 gctaaataat gctattagca atcttcgcga ggttaccaaa gagcagaatc aggcaaacat
      301 gatacccaga aagaaaacaa aatcaggtta caagggggta cacccaagag tggataacaa
      361 ggggttctgg gctacaatac gctgtaatgg aaaatctcat tatctgggtc agtttaatac
      421 agcagaagaa gctgcaaaag cctatgatgc tgcatcactg gaattgcatg gtaaatatgg
      481 acacaggaat tttagctgat agttaataaa gacctgacat aaacttgtcg ataggtctga
      541 ttaaagttta tacagtgatt tttcatcttc taattttttt ataaaaatcg tagtagcctc
      601 tctacattcc ccttcaccga taatcttcaa aaatatctac acagttgatt cggtagaatc
      661 tacctcatga ttgatggctg ttaagtaccc cagattcacg atatagcacc ccctttgcaa
      721 aagcagttaa aatatctata accaattatt gactttcttt atagcatctg ttaactccac
      781 atcaagaaac ctttatcaca cttcaaagca tctatcaaga agatttcacc tatcaataga
      841 tgtaacattt tatagttctg tgaagtccta tccagttttc tctgtaacag acacaaactt
      901 ctgtgacctt atgtatcaat gacttacaag acatgctctc aaggtgctgt atagcctctt
      961 atcttcaaat acacaaaacc cttataaatc agtactgata cgtatacagt tcgtttttaa
     1021 cataatagct gttttacgca ctaatcgtgt tctacatgaa atctgtatag atattaaggt
     1081 gtctgtaagt gattttttaa gaggagctac gaagaactat aaccatcacc tcctaacttt
     1141 cacatgggaa actgttaagc agcttgatag tcactttgag ggaatattga tagtgaccaa
     1201 ctgcatagat agcgttatat agtcctgtat atagcacttt tctaggagtc gagagaggga
     1261 cgactaacac gatacagttt accctgaaaa gagacttcac agattcttta tatatttcaa
     1321 taagttatgc taaaaaggga ctatatcaat actgcataga atctgtgtaa aactgcttag
     1381 atagtgcata aatacaccgt taaattgaat agggattcac ctgtttatga cctgttattt
     1441 acaggtatgt tctatagtta gtcttgttat gaggatgctt catccgaatc atctaccaac
     1501 ccctaatcat cctcatagtg tatttagacc ttgatgattt tagacagccg cccggttcat
     1561 gcagcacgtc cacacagtga agctattcgc gacgctcaac gaaaaaaacc taaagttccg
     1621 gtacatgctg tactgacggc gacaaatccg ctgatccgtt ttatcggtag tgatgatatg
     1681 acgcaaaacc gggaattatt tcaggtctgg ttacaaaaat tagcgcagtg gcatcagacc
     1741 actacgcctt atcttttttt acatacgcca gacattgccc aggccccgga actggtacat
     1801 accctgtggg aagacttacg taaaacgctt ccagagatcg gagcagttcc ggctattcca
     1861 cagcaatctt ctcttttctg aatttgccac ctatcataga caggtgccat cggccatttt
     1921 aaagggagtt tgtatggtaa gcgcgctgta tgccgtttta agtgcgttgt tattaatgaa
     1981 gttctctttt gatgtcgttc gcctgcgaat gcagtaccgc gttgcctatg gtgacggtgg
     2041 ttttagcgaa ctgcaaagcg ctattcgcat tcatggtaac gcggtggaat atattcccat
     2101 cgcgattgcg ctgatgctgt ttatggaaat gaatggcgca gaaacctgga tggtgcatat
     2161 ttgcggcatc gttttgcttg ctggtcgtct gatgcattat tacggttttc atcaccgtct
     2221 gttccgctgg cgacgttccg gcatgagcgc cacctggtgt gcgctgttgc tgatggtgct
     2281 ggcgaatctt tggtatatgc cctgggagtt ggttttctcc ctgcgttagc gcacaatacg
     2341 ccactttctt tttcccggat ttttacgtta tgtctcaccg cgacacgcta ttttctgccc
     2401 ctatcgccag actgggcgac tggacctttg atgaacgggt agctgaagtc ttcccggata
     2461 tgatccagcg ttccgttccc ggctattcca atattatttc catgattggt atgttagccg
     2521 agcgcttcgt tcaacctggt acgcaggttt acgatctggg ttgttctctg ggcgcggcga
     2581 cgctctcggt gcgtcgcaac attcatcatg ataattgcaa aattattgcc atcgacaact
     2641 ccccggcgat gattgaacgc tgccgtcgtc atattgacgc ctataaagcc cctacgccag
     2701 tagacgtcat tgaaggtgat attcgcgata tcgccattga aaacgcatcg atggtggtgc
     2761 tgaattttac cctgcaattc ctggaacctt ccgagcgcca ggcattactg gataaaattt
     2821 atcaaggact gaacccgggc ggcgcgctgg tgctttcgga aaaattcagt ttcgaagatg
     2881 ccaaagttgg tgaactgttg ttcaacatgc accacgactt taaacgtgcc aacggttaca
     2941 gcgaactgga gatcagccag aagcgcagca tgctggaaaa cgtgatgctg accgattccg
     3001 tggaaaccca taaagcacgc ctgcataaag ccggttttga gcatagcgag ctgtggttcc
     3061 agtgctttaa ctttggttca ctggtggcat taaaagcaga ggacgctgca tgatcgactt
     3121 tggtaacttt tattctctga ttgccaaaaa tcatctttca cactggctcg aaacgctgcc
     3181 cgcgcagatt gctaactggc agcgcgagca gcagcacggg ctgtttaagc agtggtccaa
     3241 cgcggtggaa tttctgcctg aaattaaacc gtatcgtctg gatttattgc atagcgtaac
     3301 cgccgaaagc gaagagccac tgagcaccgg gcaaattaaa cgcattgaaa cgcttatgcg
     3361 gaacctgatg ccatggcgca aagggccgtt ctcactgtat ggcgtcaaca tcgataccga
     3421 atggcgttcc gactggaaat gggatcgtgt tctgccccat ctttctgatt taaccggacg
     3481 caccattctg gatgtcggct gcggcagcgg ttatcacatg tggcgcatga ttggcgcagg
     3541 cgcgcacctc gcggtgggga tcgaccctac gcaactgttt ctgtgccagt ttgaagcggt
     3601 gcgtaaacta ctgggtaacg atcagcgcgc acatttgtta ccgttaggta ttgaacagct
     3661 tccggcactg aaagcctttg ataccgtctt ttcgatgggc gtgctttacc atcgccgttc
     3721 gccgctggag catctctggc agttgaaaga tcaactggtg aatgagggtg aactggtgct
     3781 ggaaacgctg gttattgatg gcgatgagaa cacggtgctg gtaccaggcg atcgttacgc
     3841 gcaaatgcgt aatgtctatt tcatcccttc cgcgctggcg ctgaaaaact ggctgaagaa
     3901 gtgtggtttt gtagatatcc gcattgtgga tgtgtgcgtt accactactg aagagcagcg
     3961 acgcactgaa tggatggtta ccgaatctct gtccgatttc ctcgacccac atgatccgag
     4021 taagacagtt gaaggttatc ctgcgcctaa acgcgcggtg ctgattgcgc gcaagccgta
     4081 aaggtctggt attactgccg gatgcggcgt gaatgcctta tccggcctac aaacccttgc
     4141 taattcaatc tattgcaggg actatgtagg cctggtaagc atagcgcatc aggcagtttt
     4201 acgtttgcat aacctcagcg cccgtttccg ggcgctattc acttcttacg cctgaacagc
     4261 aggcttatca aatgccgtta acttcggtgc gttacccgta tatttttcga tatacaccag
     4321 cgcagagtta ccggcacagg catttgccag ctgcgagctg gggatatccg ccgttaacac
     4381 gttcgcactg ccgtttttac acaagccgtt ttccagatct ggccatgcac cttcatgcac
     4441 gcataccacg ccttttttga tcccgtcagt caccaccgcg cctgtcaaaa tctgaccacg
     4501 tttgttccag acacgcacca gatcgccatt cgcaatacca aatcgagcag catcttcggt
     4561 gtgaatagtg atcggttcac gatcggcaac cgcatatttt ttacgcagtc ccgcatagtt
     4621 aagctgactg tgtaaacggt gtgccggatg tgcggtcaga agctgcaact gcttctcgtc
     4681 ggcggtaccc ttccactcat caggcgcaag ccaggttggg tgtgccgggc aatccttata
     4741 gccaaatttt tccagcgttt tggagtaaat ctcaattttg ccacttggcg tacccagcgc
     4801 atttttcacc ggatcggcgc ggaaatcacc ataacgaacg tactgttcgt tcttctcgct
     4861 gcggcgcatt tcgatcagtt tattttgctg ccagaaggca ttaaacattg gcatagtgac
     4921 gcgttgcgca cgggcccctt tctgagcggc atcatagaaa aatttcagcc acgccatttc
     4981 atctttacct tcggtataga tctcttttcc gccaggtttc agtaattccg caagatcggc
     5041 aaacacatca aaatcgtttc gcgcttcaaa ttgcggagca acagcctgct tcatcggcac
     5101 aatatgctgg ttgctgtaat caccggtcat cgtcaagtca ttgcgctcaa acgatgtggt
     5161 gatcggtaat acgatatctg catgtttagc cgctgcggtc cagtagcatt cagaaacgac
     5221 gatcatctcc ggtttctgcc atgctttaat cagacgatta gtatcctggt gatgggtaaa
     5281 gttaccgcca cccgcccacc agatcatttt gatattcgga tacgtttgct ctttaccgtt
     5341 atgctgatat ttgccgccag gattttccag cgcatcgacg atacgggcaa caggaatcgc
     5401 cctcattccg ccatcatcag cggcctcgct ggcctgtccg gcaatcgccg cagacatttc
     5461 cggcagcacg ccgccaacac gtgtcgggtt gccaccattt gagtaatgat aagagaaacc
     5521 aaaaccgccg cccggtgtac caatctgccc taacatcgcc gccagcgtca ccagcatcca
     5581 gtgtttctgt tcaccatatt gctggcgctg aattccccag cctgccatca gcattgtgcg
     5641 attcgccgcc atcagttcag cgagtttgac tatctgcgct tccggtacgc cagtaatttc
     5701 cgccgcccag gccgcgctct tcggcgtatt atcgctctta cctctcagat actcttcaaa
     5761 ctgcggatac ccggtagtgt atttttcaag gaacacttta tcgtgtttgc cttgtgtcat
     5821 cagggtatgc gcaatcccta acatcagtgc cacgtcggtg cccatattcg gcgcgatcca
     5881 ggtggcatta tcgccaaaga agtcgatggt ttcggagcgg ataggatcaa tggcaatcac
     5941 tggtttgcca gattttttca gctgatggaa gtattccagc ccttgctcat cggtactgct
     6001 ccaggcaatt tttaaggtat tcagcgggtt catcccccac agcaccacaa cctggctgtt
     6061 ttccagaatc agcggccagg aggtctgctg ttcatacacc tctacagaac cgaccacatg
     6121 cggcatgatc acctgtgccg ccccggttga ataatcgccg ctatgcccgg aataaccgcc
     6181 cgccaggttc atataacgtt gcagtaaggt ttgcgcttta tgcaacacgc cagaagagcg
     6241 ccagccgtat gatccggcaa aaatagccga tggtccgtta gctttgcgaa tgcgatcgtg
     6301 ttgctcatga atcagtttta atgcttgttc ccagctcacc tgtacatagg tatcttcgcc
     6361 acgacctttc gtcggttgca gtggattatc gagatagctt tttctcacca tcggatgctg
     6421 aatacgcgtc gtggtgtgta cctgatccgc cgccgtagac tgtaaggaat tcggtatggt
     6481 tttcgccagc gcgcctgttg aagaaacaat cttgccgtcc ttcacttcta cgttcatcgc
     6541 tccccaacgt cccgcggtga ggattttacc gcccttctct tctgcccatg cggggagcgg
     6601 tgctgccgat gtcaccacca gcgctccagc ggcaataccg ctgtgtttaa taaattcacg
     6661 tcttgttaat gtcataactt cctccctgat caacgaggat cactgtttct cggtaatatc
     6721 tttggcgttg tactggaaat accgcgttaa aatgtccagt tcgttttcgc tcatgctggt
     6781 tcgtgccccc attcctttgg caatggacgg ccacgcattg acggtgtaat ggtcggcggc
     6841 aataggggca tgacaaccag cgcaataggt atcgtcaagt ttttcagcgt attgccataa
     6901 cggtttacgg tccgctaatc cgggatcggt aagcgcaccc tgtaaagacg cctgacgcca
     6961 ttgattgccg tattcgtcag cctgccattc cccgtttacc gtgagcgcct tgataccttc
     7021 ttcacttaat gtggctagcg ccagccgttg acctgccgcc aggtagagag tgttttcact
     7081 gccctgcatt tgataaccct gcaacagaac gatcggctgt ttgccactgg catcaacgac
     7141 ggtgagatcg gcaccaggat tcacggtagc cagctcgcct atgcgagaag ttttgaaagg
     7201 ataaatatgt gcgccattag tcactgaagt agcggcctga ctttccagct catgcgccgc
     7261 gttgtcatcc atttttattt ctggcggaaa atgggcaatg cctttatgac aatcgataca
     7321 ggtttcgccg cccttttgtg ctttgttatg cattttctgc gcagattcac tttgcgaggc
     7381 aatatccatg gcatcaaaag aatggcaact acggcacgtt gcagagtcag ttgcttttaa
     7441 ttctttccat a
//
LOCUS       DAFNBJ010000206         7354 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000206 DAFNBJ010000000
VERSION     DAFNBJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.206"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            250..753
                     /gene="dps"
                     /locus_tag="L2X49_004403"
                     /gene_synonym="pexB"
     CDS             250..753
                     /gene="dps"
                     /locus_tag="L2X49_004403"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBN4799388.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1157..1903
                     /gene="glnH"
                     /locus_tag="L2X49_004404"
     CDS             1157..1903
                     /gene="glnH"
                     /locus_tag="L2X49_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBN4799389.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2042..2701
                     /gene="glnP"
                     /locus_tag="L2X49_004405"
     CDS             2042..2701
                     /gene="glnP"
                     /locus_tag="L2X49_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBN4799390.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2698..3420
                     /gene="glnQ"
                     /locus_tag="L2X49_004406"
     CDS             2698..3420
                     /gene="glnQ"
                     /locus_tag="L2X49_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBN4799391.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3681..5906
                     /gene="ybiO"
                     /locus_tag="L2X49_004407"
     CDS             3681..5906
                     /gene="ybiO"
                     /locus_tag="L2X49_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HBN4799392.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(5903..6829)
                     /gene="rlmF"
                     /locus_tag="L2X49_004408"
     CDS             complement(5903..6829)
                     /gene="rlmF"
                     /locus_tag="L2X49_004408"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBN4799393.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISSNPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7105..>7354
                     /locus_tag="L2X49_004409"
     CDS             7105..>7354
                     /locus_tag="L2X49_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBN4799394.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAT"
BASE COUNT         1768 a         1867 c         1856 g         1863 t
ORIGIN      
        1 ttttcctcta caccgtcttt atatatcgaa ttatgcaaaa gcatatttat tcctaaaatt
       61 cctggcgagc agataaataa gaattgttct tatcaatata tctaactcat tgaatcttta
      121 ttagttttgt ttttcacgct tgttaccact attagtgtga taggaacagc cagaatagcg
      181 gaacacatag ccggtgctat acttagtctc gttaattact gggacataac atcaagagga
      241 tatgaaatta tgagtaccgc taaattagtt aaatcaaaag cgaccaatct gctttatacc
      301 cgcaacgatg tctccgacag cgagaaaaaa gcaacagtag agttgctgaa tcgccaggtt
      361 atccagttta ttgatctttc tttgattacc aaacaagcgc actggaacat gcgcggcgct
      421 aacttcattg ccgtacatga aatgctggat ggcttccgca ccgcactgat cgatcatctg
      481 gataccatgg cagaacgtgc agtgcagctg ggcggtgtag ctctggggac cactcaagtt
      541 atcaacagca aaaccccgct gaaaagttac ccgctggaca tccacaacgt tcaggatcac
      601 ctgaaagaac tggctgaccg ttacgcaatc gtcgctaatg acgtacgcaa agcgattggc
      661 gaagcgaaag atgacgacac cgcagatatc ctgaccgccg cgtctcgcga cctggataaa
      721 ttcctgtggt ttatcgagtc taacatcgaa taaatccatc gctgatggtg cagaacttta
      781 gtacccgata aaagcggctt cctgacagga ggccgttttg ttttgcagcc cacctcaacg
      841 cacttattta gtgcatccat ctgctatctc cagctgatta agtaaatttt ttgtatccac
      901 atcatcacac aatcgttaca taaagattgt tttttcatca ggttttacgc taaataatca
      961 ctgtgttgag tgcacaattt tagcgcacca gattggtgcc ccagaatggt gcatcttcag
     1021 ggtattgccc tataaatcgt gcatcacgtt tttgccgcat cttgaaaaat caatgagttg
     1081 caaaactggc acgatttttt catatatgtg aatgtcacgc aggggatcgt cccgtggata
     1141 gaaaaaagga aatgctatga agtctgtatt aaaagtttca ctggctgcac tgaccctggc
     1201 ttttgcggtt tcttctcatg ccgcggataa aaaattagtt gtcgcgacgg ataccgcctt
     1261 cgttccgttt gaatttaaac agggcgataa atatgtgggc tttgacgttg atctgtgggc
     1321 tgccatcgct aaagagctga agctggatta cgaactgaag ccgatggatt tcagtgggat
     1381 cattccggca ctgcaaacca aaaacgtcga tctggcgctg gcgggcatta ccatcaccga
     1441 cgagcgtaaa aaagcgatcg atttctctga cggctactac aaaagcggcc tgttagtgat
     1501 ggtgaaagct aacaataacg atgtgaaaag cgtgaaagat ctcgacggga aagtggttgc
     1561 tgtgaagagc ggtactggct ccgttgatta cgcgaaagca aacatcaaaa ctaaagatct
     1621 gcgtcagttc ccgaacatcg ataacgccta tatggaactg ggcaccaacc gcgcagacgc
     1681 cgttctgcac gatacgccaa acattcttta cttcatcaaa accgccggta acggtcagtt
     1741 caaagcggta ggtgactctc tggaagcgca gcaatacggt attgcgttcc cgaaaggtag
     1801 cgacgagctg cgtgacaaag tcaacggcgc gttgaaaacc ctgcgcgaga acggaactta
     1861 caacgaaatc tacaaaaaat ggttcggtac tgaaccgaaa taataacgct acacctgtaa
     1921 aacgcactgg cagttccctc tcccctatgg ggagaggatt agggtgaggg gcgcaaaccc
     1981 gctccggggc cattaattac cctgaatttg attatttaca ccacggtaac aggaacaaca
     2041 tatgcagttt gactggagtg ccatctggcc tgccattccg cttctgattg aaggtgccaa
     2101 aatgaccctg tggatttcgg tcctcggtct ggcaggcggt ctggtaatcg gattgctggc
     2161 aggttttgca cgcaccttcg gtggttggat agccaaccac gtcgcgctgg tctttattga
     2221 agtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt atctatttcg ccctgccgat
     2281 ggcgtttaac gacttacgca tcgacccatt tactgcggcg gtggtcacca tcatgatcaa
     2341 ctccggcgcg tacattgcgg aaatcacgcg tggtgcggtg ctgtctattc acaaaggttt
     2401 tcgtgaagca ggactggcgc tcggtctttc acgttgggaa accattcgct acgtcatttt
     2461 accgctggca ctgcgtcgta tgctgccgcc gctgggtaac cagtggatca tcagcattaa
     2521 agacacctcg ctgtttattg ttatcggcgt ggcggaactg acccgtcagg ggcaagaaat
     2581 tattgccggt aacttccgcg cccttgagat ctggagcgcc gtggcggtgt tctatctgat
     2641 tattaccttg gtgctgagct ttattctgcg tcgtctggaa agaaggatga aaatcctgtg
     2701 attgaattta aaaacgtctc caagcatttt ggtccaaccc aggtgctgca caatatcgat
     2761 ttgaacattg ctcagggcga agtcgtggtg attatcgggc cgtccggttc cggtaaatcg
     2821 accctgctgc gctgcatcaa caaactggaa gaaatcacct ccggcgatct gattgtcgat
     2881 ggcctgaagg ttaacgatcc gaaagttgac gagcgcctga ttcgccagga agcgggtatg
     2941 gtgttccagc agttttacct cttcccgcat ctgacggcgc tggaaaacgt catgtttggt
     3001 ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa aactggcacg tgagctgctg
     3061 gcgaaagtcg gtctggcaga acgtgcacat cactaccctt ccgaactttc tggtggtcaa
     3121 cagcagcgtg tggcgattgc ccgcgcgctg gcggtgaagc cgaaaatgat gctgtttgat
     3181 gaaccgactt ccgctcttga cccggaactg cgccatgaag tgctgaaggt tatgcaggat
     3241 ctggctgaag aagggatgac gatggtgatc gtgacccacg aaatcggttt tgccgagaaa
     3301 gtagcttcgc ggctgatctt tatcgacaaa ggccggattg cggaagatgg caatccgcag
     3361 gtgttgatca agaacccgcc gagccagcgc ttgcaggaat ttttgcagca cgtctcttaa
     3421 taagacacat ttcctgatgc gctacgctta tcaggcctac aggatatctg gcaacttatt
     3481 aaaattgcat gaacttgtag gacggataag gcgttcacgc cgcatccggc aaaaaagccc
     3541 gcacgttgtc agcaacctgc ttaatatccc ttcctccctt tcacccgaaa gggaggcaca
     3601 ccagattcct ctcatttaaa atcgcccctc ctccagcatc tatacttatc tttttgctct
     3661 attttctcac tggaggagtc atgcggtgga tcctgttcat cctcttctgc ctgctgggcg
     3721 cacctgccca cgcggtatcc atacccggcg ttacaaccac aacgacaacg gactcaacga
     3781 ctgaaccggc cccggaaccg gatatcgaac aaaaaaaagc ggcctatggc gcactggcgg
     3841 atgtgctgga taatgacacc tcgcgtaaag agttgatcga ccagttgcgc accgttgccg
     3901 ctacgccccc tgctgaaccg gtaccaaaaa tagtgccgcc gacgctggtc gaagagcaaa
     3961 ccgtgctgca aaaggtcacc gaagtcagcc gccattatgg tgaagccctt tccgcccgct
     4021 tcgggcaact ttatcgcaat atcaccggct ccccgcataa gccgtttaat ccacaaacct
     4081 tcagcaatgc gctgacccat ttttcaatgt tagcggtatt agtgtttggt ttttactggc
     4141 tgattcgcct gtgcgcactg ccgctgtatc gcaaaatggg tcagtgggcg cggcaaaaaa
     4201 atcgtgagcg cagcaactgg ttgcagcttc cggcgatgat tatcggggcg tttattatcg
     4261 acctgctgtt actggccctg acattatttg tcggccaggt attaagcgac aacctgaatg
     4321 caggcagtcg caccatcgct ttccaacaaa gtttgtttct caacgccttt gccctcattg
     4381 aatttttcaa agccgtacta cgcctgattt tttgcccaaa cgtggcggag ctgcgcccgt
     4441 tcacgattca ggacgagagc gcccgttact ggagtcgtcg cctgagctgg ttaagcagtt
     4501 tgataggtta tggcctgatt gtggccgtgc cgattatctc taatcaggtg aatgtacaga
     4561 taggtgcgct ggcgaacgtc atcattatgc tgtgcatgac cgtctgggcg ttgtacctga
     4621 tctttcgtaa taaaaaagag attacccagc atttgctcaa cttcgcggag cattcgctgg
     4681 cctttttcag cctgtttatc cgcgcctttg cgctggtgtg gcactggctg gcaagcgcct
     4741 attttatcgt gctgtttttc ttttcgttgt tcgatccggg caacagcctg aaatttatga
     4801 tgggtgcaac ggtgcgcagc ctggcgatta ttggtatcgc agcgtttgtt tccggtatgt
     4861 tttcccgctg gctggcgaaa accatcactc tctcgccaca tactcagcgt aactatccgg
     4921 agctgcaaaa acggttgaat ggctggctgt cggcggcgct gaaaacggcg cgtattctga
     4981 cagtctgcgt ggcggtaatg ttgctgttga gcgcatgggg attgttcgat ttctggaact
     5041 ggctgcaaaa cggcgcgggg cagaaaaccg tagatatcct gatccgtatc gcactcattc
     5101 ttttcttctc ggcggttggc tggacggtgc tcgccagttt gatcgaaaac cggctggctt
     5161 cggatattca tggccgcccg ctacccagcg cccgcacgcg taccctgctt acgctgtttc
     5221 gtaacgcgct ggcggtgatt atcagtacca tcaccatcat gattgtgttg tcggaaatcg
     5281 gcgtcaatat cgcgccattg ctggcaggtg ccggggcatt aggtctggct atctcgtttg
     5341 gttcgcaaac gctggtgaaa gatattatca ccggggtatt tattcagttt gaaaacggca
     5401 tgaacactgg agatttggtg actatcgggc cgttgaccgg cacagtggaa cggatgtcga
     5461 ttcgctccgt gggcgtacgc caggataccg gggcgtatca catcattccg tggtcttcga
     5521 taaccacctt tgctaacttt gtccgcggca ttggttcggt ggtggcaaat tatgatgttg
     5581 atcgccatga agatgctgat aaagccaatc aggcactgaa agatgcggta gcggaattaa
     5641 tggaaaacga agaaattcgc gggctgatta ttggtgaacc gaattttgcc gggattgtcg
     5701 gcttaagcaa taccgcgttt acactgcgtg tttcgttcac cacgctgcca ctcaaacagt
     5761 ggacggtacg ctttgccctc gacagccagg tgaaaaaaca tttcgacctg gcgggcgttc
     5821 gcgcgccagt gcagacttat caggtgctgc ctgctccggg cgcgaccccg gctgaaccgt
     5881 taccgccggg ggaaccaacg ctttaacgct ggcgattgac aaaacggcgg cgctgctcgt
     5941 cgttcataaa ggtccaggca ataaagcgac tctgcttttg cccctgggcc atctcttttt
     6001 taaccacctt caccgcgccc acgtccgtca gggcacgata caacggcggt aagttttcac
     6061 cacgagatac cagtgatgta aaccacatca cctgcttcgc gaagcctttg ctctcttcaa
     6121 tcatcttttt gataaaggtg acttcaccgc cttcacacca caactcctgt tgctggccgc
     6181 caaagttcag tgcatcgtct ttgttcagcc ccaggttacg gcgtttacgc tcactacctg
     6241 cccgtgccgc agcagcggaa tcgtggaatg gcgggttaca caaggtcgcg tcgtattgct
     6301 cgtttttatg gatgatgccg ttaaaaatcg ccccactctc tttttgccga cgcagacgaa
     6361 tggcgcggtt aagccccgga ttagaactga taatcgcctg cgcactgctt aacgcctggc
     6421 tgctggtttc actaccggta aaacgccagc catattcatg tacgccaatc agcggataaa
     6481 tacagttcgc accaacgccg atatccagaa tgctggcatt cgctggaatt gttccgcttg
     6541 cttctgccag taaatcggca aggtgatgaa tataatccgc ccggcccggt actggtgggc
     6601 agagaaaacc gtcggggata tcccagttcg ctacggcgta aaaatgggcc agcaacgcct
     6661 tattgagcgc cttcaccgcc agcggattgg caaagtctac gctttgctcc ccggcgggtg
     6721 taagcgtgag gaattgcctg agttcaggat tgacctgaca aagcgtggcg agatcgtagc
     6781 ggctgtgatg acggttgcgc ggatgcaacc ccggtttctg ggcggacata gcattctcct
     6841 ttaagctgcg gcgtaagata cccgcataac gcgacagggt aaatatctgg ctgcgtttat
     6901 cattccccaa aaccttaaca tggctatggt ttatggcaat tcatcataaa aatcagcatt
     6961 atgcatgtca ttgatttgtc ttattttgta aaaatatatc gttcaaaaaa taagcacatc
     7021 tctgtcaatt ttttgcgtca tttgcatcgc gcaggcgacc tacacttatc aggcactacc
     7081 cgttgtattt atgaggatgt cattatgaaa aagtgcctca cactactgat tgccaccgtc
     7141 ctgagcggaa tctctctcac ggcttatgcc gcgcaaccga tgagtaacct ggacagcggt
     7201 cagctacggc ccgccggtac cgtttcggca actggcgcat caaacctaag cgatctggag
     7261 gataaactgg cagaaaaagc gcgcgaacaa ggcgcgaaag gttatgtcat taattccgca
     7321 ggcggaaatg accagatgtt cggtactgca acca
//
LOCUS       DAFNBJ010000207         7322 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000207 DAFNBJ010000000
VERSION     DAFNBJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.207"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(253..1092)
                     /locus_tag="L2X49_004410"
                     /pseudo
     CDS             complement(253..1092)
                     /locus_tag="L2X49_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1326..2867)
                     /locus_tag="L2X49_004411"
     CDS             complement(1326..2867)
                     /locus_tag="L2X49_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBN4799395.1"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPA
                     VSEKRLALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVF
                     APNSKHRVQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVC
                     EHCGGHVKVIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQ
                     SRLFD"
     gene            complement(3272..4111)
                     /gene="sul1"
                     /locus_tag="L2X49_004412"
     CDS             complement(3272..4111)
                     /gene="sul1"
                     /locus_tag="L2X49_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HBN4799396.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(4105..4452)
                     /locus_tag="L2X49_004413"
     CDS             complement(4105..4452)
                     /locus_tag="L2X49_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HBN4799397.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(4616..5407)
                     /locus_tag="L2X49_004414"
     CDS             complement(4616..5407)
                     /locus_tag="L2X49_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /protein_id="HBN4799398.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQ
                     DLFEALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
     gene            complement(5413..5703)
                     /locus_tag="L2X49_004415"
     CDS             complement(5413..5703)
                     /locus_tag="L2X49_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1010 domain-containing protein"
                     /protein_id="HBN4799399.1"
                     /translation="MFIQTAFSFSGVIQCLFCLFSGLRLHGLRRFSVFLASSPCVASA
                     SSYRFCSAVPPRWRSVFSRLAPVAKFKLSVLASGSNISVKPTRILRSAYLAR"
     gene            complement(5815..6312)
                     /gene="dfrA12"
                     /locus_tag="L2X49_004416"
     CDS             complement(5815..6312)
                     /gene="dfrA12"
                     /locus_tag="L2X49_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /protein_id="HBN4799400.1"
                     /translation="MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGK
                     VVVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVA
                     GGAEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELVSTETIQAVIPYTHSVY
                     ARRNG"
     gene            6457..>7322
                     /gene="intI1"
                     /locus_tag="L2X49_004417"
     CDS             6457..>7322
                     /gene="intI1"
                     /locus_tag="L2X49_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBN4799401.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLR"
BASE COUNT         1617 a         1995 c         2029 g         1681 t
ORIGIN      
        1 tcggtttttc ccccgaacta cgactgtttc gcaccccgaa caactaatcc aatcagaatt
       61 ttccggcaac gccgactcta agaagtgtaa ttccagtcca tttctgccgt caacccctga
      121 ttcgcgttaa tgcgacaaag ctcaaacaca taactccccc acgaagccat ataaactccg
      181 atttcgaggt gttatcggcg ttgttgacca aatccgtcgc tggatggccg ttccccaacc
      241 cgttgatacc actcaattca ctggctggcg aacaggcata ccaagcccta tcagcttgtt
      301 catcaccttc acgccagcca ggatttcgcc cacctgggcg ttgtagttcc gcaggctcag
      361 cgttggcccg atgagttgct tgaaccggta catggcggtc tcggctatcg agcgctggtg
      421 atagccggag tctttcttcc attgcttcag ttcaccggcc ttgagcgccg tcaccgcctc
      481 gttgcgggga tggccttcct cccacggtgc cgcatttttt ctcggcggta tggtggcttt
      541 cgcccccttt ttcttcagta gcgcatgaca ggccttggtg tcatacgcac catcggcgct
      601 gacctgtttt atcttgcgtc gtaaggggtt gagcaacgtc ggtaacacct cgttatccgc
      661 caccgtctcc aggctgactt cggcagcgat gaccgcatgg gttgtggcat ccaccgccaa
      721 gtggagcttg cgccacactc gtcgcttctc cttgccgtgt ttgcgaattt tccattcgcc
      781 ttcgccatag accttgaggc cggtggcatc aatgaccaag tgagccaccg ggccttggct
      841 ggggaggcgg tacttgatgt cgacggtctt ggcgcgcttg ctgatacagc tatagtccgg
      901 cgactgcaaa ggcaccgcca tcagttggaa caacgagtta atgaagcctt ctagcgcccg
      961 cagcggcaac ttgaacaagc cttttagcat cagggccgtc tcaatggcgg tgtcactgta
     1021 gtggaaacct ctccctcggc ggccatggtg gaccaggcaa tgccactgct taatggcttg
     1081 ttcgtccatc caggggtata ggaagtataa accacctttt tgctcctcat ccgaagtatc
     1141 ttacctgaaa ttccctcact cgtttaccgc tcaagcccca attttaactg ccggtccagc
     1201 ctaaaccgct ctaataaggt tcgatttggc ggtaaaatct ctagcctgat agctcgagag
     1261 atacaaactg ccccaccgcc ccgtttaaaa gttggcagtg ttgagcagtg ttggatttgg
     1321 ggtcgtcagt caaagagacg actctgtgat ggatcgaaca ggctgggagt cagtggcggc
     1381 gctcgttctg gtggcagctc acgctgcttg gcggcattcg ccttggctgt tttctgtttc
     1441 agatgcttga gaatctgctc aatgaccttc ggatcttcga tgctggcaat cactttgacg
     1501 tgaccgccgc agtgttcgca gacttcaata tcaatattga agactcgctt gaggcgttgc
     1561 atccaggtca tggcgcggtg gcgctctgca ggactcttgt cacgccaatt agtatcgaga
     1621 ccttccgatt tgtcgggctt cttgccccgc ttggcgggtg ttacttgaac tcggtgtttg
     1681 ctgttcggtg caaagacgcc gtggaagcgt gtgaggttga ctcgcggctt aggtaccaac
     1741 gcagcgagtt tggcgatgaa gtccagcggc tcgaagatca catgggtggt gccattgcgg
     1801 tacggagttt tgagctcgta acgcacctgc ccattggcgg ttaatgccag acgtttttct
     1861 gaaaccgctg gccgactaat gtagcgacac aagcgctcaa gcttatcccg ctgatgcgct
     1921 tcggccatca caccggcgtg tagcgagaaa ccagcatggt tggctactcg actgcttgag
     1981 tcggctttat cctcacgccc tggcaaggtt tgcagggtga agactttgcg cccttgctgg
     2041 gggccgacgg caatgcgata cgtaaccgaa gcaccatgta attgagtcag cgtatcgtct
     2101 tcgccctctt ccagtgtcaa ccacgtattc tcggcatcac gctccaaaat cccacgcttt
     2161 tccatgcagc gagcgatgcg atggctgagg gtgtgagcga gcgtattcag ctcatcgtaa
     2221 gtgggtgcct tgacacgatg gaagcgttgc ttgccatagt catcttcggc atagacacca
     2281 tcgagaaaca gcatgtggta gtggacattg agatttagcg cggagccaaa gcgttggata
     2341 agagtcactg agccagtttg tgcagaggct ttggtgtaac cggctttttt gatcagatga
     2401 gttgagagtg tacgatagac gatactcaag acctggccca tcagctgggg atggcgagcc
     2461 agcaaaaagc gtagctggaa aggaaagctg agcacccact ggcgaatggg ctccttgggg
     2521 aagacttcgt ctatcagcag cgccgcactc tcggccatcc ggcgggcacc gcagctaggg
     2581 caaaagccgc gtcgtttaca gctgaaggcg accagacgct cgtgatgaca atcctcgcag
     2641 cgaacccgca tgaaaccata ctccagacgg ccacattgga ggaggtcgtt gaattcttgt
     2701 tggatgtagc gaggcaggtg ttgaccttgg gcttcgagtg aggctttgaa ggctgggtag
     2761 tgctgctcaa ccagctggta gagcagcgtc tggtcgggtt ggtggcgttc gtaaccgttt
     2821 gtttgagtgg gcgattgact cgccgtggcg ttccttgcca gcgacatggg tatcctccgc
     2881 tgatactgtg gttatgtaca gtatcagcgg cttgcgttca gacgtccagt ctggccctag
     2941 acatcgctaa atgcttaacc cgcaatagcc ctcacgagtt gttatcagcc actaccggtt
     3001 gagcgagaag gttttgggtt cagggtgcta ttgctccacc aatcacaata ctgaagcccc
     3061 aactgttatc agttggggct ttttcttgtc tgtttgcggc ggttgcgttt tatcggtagt
     3121 cgtcgagctc tgcaccatcc cacataagag cttaacggtg cgatcttcaa cgccatcaca
     3181 caaaactttc tttttcacgc acagtcaact tattggatgt tttattaaca acccaaaagg
     3241 agatatttag cgggcggccg gaaggtgaat gctaggcatg atctaaccct cggtctctgg
     3301 cgtcgcgact gcgaaatttc gcgagggttt ccgagaaggt gattgcgctt cgcagatctc
     3361 caggcgcgtg ggtgcggacg tagtcagcgc cattgccgat cgcgtgaagt tccgccgcaa
     3421 ggctcgctgg acccagatcc tttacaggaa ggccaacggt ggcgcccaag aaggatttcc
     3481 gcgacaccga gaccaatagc ggaagcccca acgccgactt cagcttttga aggttcgaca
     3541 gcacgtgcag cgatgtttcc ggtgcggggc tcaagaaaaa tcccatcccc ggatcgagga
     3601 tgagccggtc ggcagcgacc ccgctccgtc gcaaggcgga aacccgcgcc tcgaagaacc
     3661 gcacaatctc gtcgagcgcg tcttcgggtc gaaggtgacc ggtgcgggtg gcgatgccat
     3721 cccgctgcgc tgagtgcata accaccagcc tgcagtccgc ctcagcaata tcgggataga
     3781 gcgcagggtc aggaaatcct tggatatcgt tcaggtagcc cacgccgcgc ttgagcgcat
     3841 agcgctgggt ttccggttgg aagctgtcga ttgaaacacg gtgcatctga tcggacaggg
     3901 cgtctaagag cggcgcaata cgtctgatct catcggccgg cgatacaggc ctcgcgtccg
     3961 gatggctggc ggccggtccg acatccacga cgtctgatcc gactcgcagc atttcgatcg
     4021 ccgcggtgac agcgccggcg gggtctagcc gccggctctc atcgaagaag gagtcctcgg
     4081 tgagattcag aatgccgaac accgtcacca tggcgtcggc ctccgcagcg acttccacga
     4141 tggggatcgg gcgagcaaaa aggcagcaat tatgagcccc atacctacaa agccccacgc
     4201 atcaagcttt tgcccatgaa gcaaccaggc aatggctgta attatgacga cgccgagtcc
     4261 cgaccagact gcataagcaa caccgacagg gatggatttc agaaccagag aaagaaaata
     4321 aaatgcgatg ccataaccga ttatgacaac ggcggaaggg gcaagcttag taaagccctc
     4381 gctagatttt aatgcggatg ttgcgattac ttcgccaact attgcgataa caagaaaaag
     4441 ccagcctttc atgatatatc tcccaatttg tgtagggctt attatgcacg cttaaaaata
     4501 ataaaagcag acttgacctg atagtttggc tgtgagcaat tatgtgctta gtgcatctaa
     4561 cgccggagtt aagccgccgc gcgtagcgcg gtcggcttga acgaattgtt agacatcatt
     4621 taccaactga cttgatgatc tcgcctttca caaagcgaat aaattcttcc aagtgatctg
     4681 cgcgtgaggc caagtgatct tctttttgtc ccagataagc ttgcttagct tcaagtaaga
     4741 cgggctgata ctgggcaggt aggcgtttta ttgcccagtc ggcagcgaca tccttcggcg
     4801 cgattttgcc ggttattgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct
     4861 catcgccggc ccagtcgggc tgcgagttcc atagcttcaa ggtttccctc agcgcctcga
     4921 atagatcctg ttcaggaacc gggtcaaaga attcctccgc tgccggacct accaaggcaa
     4981 cgctatgttc tcttgctttt gtaagcagga tagctagatc aatgtcgatc atggctggct
     5041 cgaagatacc cgcaagaatg tcattgcgct gccattctcc aaattgcagc tcgcgcttag
     5101 ccggataacg ccacgggatg atgtcgtcat gcacgacaag ggtgacttct atagcgcgga
     5161 gcgtctcgct ctcgccaggg aaagccgaag cctccataag atcattgagc aatgctcgcc
     5221 gcgtcgtttc atcaagcttt acggccacag taaccaacaa atcaatatcg ctgtatggct
     5281 tcaggccgcc atccactgcg gagccgtaca aatgcacggc cagcaacgtt gattccagat
     5341 ggcgctcaat gacgcttagc acctctgata gttggttcga aatttcgatg gtcaccgctt
     5401 ccctcatgat gtctaacggg cgaggtaagc cgaccgcaga atgcgggtcg gcttgaccga
     5461 aatgttagaa ccagaagcca aaacggataa cttgaatttg gcgacgggcg ctaaccgtga
     5521 aaaaacgctg cgccaccgag gcggcacagc actgcaaaaa cgatagctgc ttgcgcttgc
     5581 tacgcaaggg ctagaggcca aaaagactga aaacctgcgc agcccatgca ggcgaagccc
     5641 ggaaaaaagg caaaacaggc actgaataac gcctgaaaag ctaaatgccg tttgaataaa
     5701 catgagctaa ataaagctgg gtttcagtgg tgctaacgtt ggacgtaacg agagccggag
     5761 cgcagcggag ggaaccaaaa tgcgcagcat tttggcgtcc cgttgacgga atggttagcc
     5821 gtttcgacgc gcataaacgg agtgggtgta cggaattaca gcttgaatgg tttcggttga
     5881 gacaagctcg aattctgttt cgttgagcat tgggaagaag gcgtcaccct cgaaggtttg
     5941 atgtacctca gatagaaaca cgccgtgggc gtgaggtagt gccagagtgt atatctcagc
     6001 tccgcccgcg acgtagagtt cattgccgag ttcggatgcc aaagcgatag cgtgcgacag
     6061 cgttgaaaca actacgcagc cagtggcgcg gtagttagct tggcgtgaga ttaccaatgt
     6121 gtgacggttc ggtagaggct tgccgataga ctcaaaggtc tttcgcccca tgacaacgac
     6181 ttttccctca gtgagtctgc gaaaaatctt ctgctcaccc ggaattttcc aggggatatt
     6241 aggaccattg ccaataaccc gattggctcc catcgcagca acgagataaa tgcgtactga
     6301 ttccgagttc atatggctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     6361 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     6421 ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     6481 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     6541 tacagcttac caaccgaaca ggcttatgtc cactgggttc gtgccttcat ccgtttccac
     6601 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
     6661 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
     6721 ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg
     6781 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
     6841 ctggaaggcg agcatcgttt gttcgcccag cttctgtatg gaacgggcat gcggatcagt
     6901 gagggtttgc aactgcgggt caaggatctg gatttcgatc acggcacgat catcgtgcgg
     6961 gagggcaagg gctccaagga tcgggccttg atgttacccg agagcttggc acccagcctg
     7021 cgcgagcagc tgtcgcgtgc acgggcatgg tggctgaagg accaggccga gggccgcagc
     7081 ggcgttgcgc ttcccgacgc ccttgagcgg aagtatccgc gcgccgggca ttcctggccg
     7141 tggttctggg tttttgcgca gcacacgcat tcgaccgatc cacggagcgg tgtcgtgcgt
     7201 cgccatcaca tgtatgacca gacctttcag cgcgccttca aacgtgccgt agaacaagca
     7261 ggcatcacga agcccgccac accgcacacc ctccgccact cgttcgcgac ggccttgctc
     7321 cg
//
LOCUS       DAFNBJ010000208         7320 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000208 DAFNBJ010000000
VERSION     DAFNBJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.208"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(319..1455)
                     /locus_tag="L2X49_004418"
     CDS             complement(319..1455)
                     /locus_tag="L2X49_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBN4799402.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1458..1820)
                     /locus_tag="L2X49_004419"
     CDS             complement(1458..1820)
                     /locus_tag="L2X49_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBN4799403.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2357..4270
                     /gene="mtlA"
                     /locus_tag="L2X49_004420"
     CDS             2357..4270
                     /gene="mtlA"
                     /locus_tag="L2X49_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBN4799404.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4500..5648
                     /gene="mtlD"
                     /locus_tag="L2X49_004421"
     CDS             4500..5648
                     /gene="mtlD"
                     /locus_tag="L2X49_004421"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBN4799405.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5648..6235
                     /gene="mtlR"
                     /locus_tag="L2X49_004422"
     CDS             5648..6235
                     /gene="mtlR"
                     /locus_tag="L2X49_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBN4799406.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6247..6456)
                     /locus_tag="L2X49_004423"
     CDS             complement(6247..6456)
                     /locus_tag="L2X49_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799407.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6741..7103
                     /locus_tag="L2X49_004424"
     CDS             6741..7103
                     /locus_tag="L2X49_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBN4799408.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1873 a         1832 c         1849 g         1766 t
ORIGIN      
        1 tagattaaat attttttaaa tgtgactgtc atccttcaga taataaagcc atcatcgcaa
       61 tagatggaaa agcactcctg ctcttttttg attagagccg tcgcaggagg aacaactcat
      121 atcatcattg cgatctcgac gatgtatagt ctcatcctgc ggcagaacaa gacggataaa
      181 aaatccaatg agagcacact catccagaac ttattaacat aatgaatatt aaagaaaaat
      241 catcgcagct aatgaagtaa tatgcaaaaa taattagtcc ttacaaggag caaagttcca
      301 taatctcccc cctccccctc aatgatccaa ataaagataa tgcatccagc tggtcattct
      361 tagcagcact ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa ccgccacctg
      421 ggcgtagatg ccgtcgggta aggcatcgat atcatcgtta gggtccagtt caatggttcc
      481 cagcacaccg tccgtgccgg gcacgaccgt taatgattgc aataccccct gcgcctgata
      541 agaaccgcct ggcacgacag gtaaaatact agtcagtttg ccgtgaaaca cctgcccagg
      601 tagcgcgtta aacaccactt ccgcatcatc gccgggtttc agacgtaaca gcgagttttg
      661 ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aagaccatca ccggacgcag
      721 cggcaaggca gctgcgtatg tacctgggcg gatcagtacc tgagtaacgt agccattgct
      781 cggcgcgcga atgacagtct gctcaaggtt atattttgct tcagtaagtt gcgctcttaa
      841 gctcacaatc tgcgattgct cgccgttaac catactgtcg agctggctct ggatctgcgc
      901 ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg aggaaatttt gccgcgcatc
      961 gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca agtaacgttg
     1021 ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc
     1081 agtgagctgc gcacgcagcg tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag
     1141 tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct cgcccttttg
     1201 aataagctga ttattcttgt cagtgacttc agtaacaatt cccgtcacct gtggcgtgat
     1261 agggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt agttcatcaa
     1321 caaaattaaa ccactcacca gaaacacgcc tcccagcgcc gccgtcgcca gcgtccactg
     1381 atttaccgga atgcggaaga ttttaaagac cgcccacgcc agcgccacgt aagttaaaac
     1441 aatcaataga tccataatta gatctccggg aacgtggaat agtcagtctt tttctcggcg
     1501 gcgagttgat gctccagccg ggaaattcgg tcagaaagtg aggcgatttc cgggtcagtc
     1561 gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg ttgatagagc
     1621 gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct cacccagccc
     1681 gccgtatgaa tagcgtcggc atgggggtga ttgcgctttt tggcaatcag ataagggatg
     1741 tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag cactcccaac
     1801 gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt gtttttgggc
     1861 tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg cgattctcgc
     1921 cataacacca aagaataatt tttagaggtg atgagttgct tagttacata acgattgtat
     1981 gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa atatcttgtg
     2041 attcagatca caaagattca acaaaccatt aaaacaaaaa tgtgacacta ctcacattta
     2101 aatgccattt ttagcgaaaa tcgccgtctt gttgcttttt tacacaagcg ttttgtgatg
     2161 aacgtcacgt caattacctc tctaccccct atatttatgt gattgatatc acacaaaagg
     2221 ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac aaggtccggc
     2281 tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga cagcaaacat
     2341 aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct ttggtcgttt
     2401 cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc
     2461 gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat
     2521 gatcacttat ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg taggcggcga
     2581 acgtggcggc gtagtcggtg ccatcactac catgggcgtt atcgtcggcg cagacatgcc
     2641 gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta agcacttcga
     2701 ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata acttctccgc
     2761 aggcatcatc gggatgatcc tcgccattct ggcatttctc ggcattggcc cgattgttga
     2821 agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg acatgctgcc
     2881 gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg ccattaacca
     2941 cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat caatcttctt
     3001 cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg
     3061 tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc atccacttcc tggggggtat
     3121 ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct
     3181 cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg tttctccggc
     3241 atctccgggt tctatccttg ctgtactggc gatgacacca aaaggtgctt atttcgctaa
     3301 catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa
     3361 aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta tgcaggacat
     3421 gaaagctgag tctaaaggca catctccgct gtctgcgggc gatgtgacta acgacctgag
     3481 ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc
     3541 aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca cagatttcgg tcactaacag
     3601 cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg acctgaccga
     3661 acgcgctatg cgccaggttc cgcaggcgca gcatatttcg ctgaccaact tcctcgacag
     3721 cggcctgtac accagcctaa ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga
     3781 agtgaaagtg aaagacagtc tgaaagacag ctttgacgat tccagtgcta acctgttcaa
     3841 actgggcgcg gagaacatct tcctcggtcg caaagcagca accaaagaag aagcgattcg
     3901 ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat
     3961 gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg tgccacacgg
     4021 tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga
     4081 aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt ctggtgattg gtattgcagc
     4141 ccgtaacaac gagcacattc aggttatcac cagcctgacc aatgcactgg atgatgagtc
     4201 cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac tgctggcagg
     4261 tcgtaagtaa tccaatccca ccctctccac atggagaagg tggggttaat tgcctgatgc
     4321 gctacgctta tcaggcctac aggatgcatc acaatttgtt gaatttgcac gttcttgtag
     4381 gccggataag gcgcttacgc cgcatccggc gcggcccctc tcctcacgga gagggtttgg
     4441 gtgagggaaa agcctcaccc cagccctctc gggtaaaaac attgatgaag gttaatacta
     4501 tgaaagcatt acattttggc gcaggtaata tcggtcgtgg ctttatcggt aaactgctgg
     4561 cagacgcggg tatccaactg acgtttgccg atgtcaatca ggtggtactt gatgccctga
     4621 atgcccgtca tagctatcag gttcatgtgg ttggtgaaac cgagcaggta gataccgttt
     4681 ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt tgatctgatt gctcaggtcg
     4741 atttagtcac taccgccgtt ggcccggttg tgctggaacg tattgctccg gctatcgcca
     4801 aagggctggt gaaacgcaaa gaacaaggta atgaatcccc gctgaacatc atcgcctgtg
     4861 aaaacatggt acgcggtacc acgcagctga aaggccatgt gatgaacgcc ctgccggaag
     4921 acgccaaagc gtgggtagaa gaacacgttg gctttgtcga ttcagccgtt gaccgcatcg
     4981 taccgccttc ggcttcggca actaacgatc cgctggaagt gacggtagaa actttcagcg
     5041 aatggattgt cgataaaacg cagttcaaag gcacactgcc gaacatccca ggcatggagt
     5101 taaccgacaa cctgatggca tttgtcgaac gtaaactctt caccctgaac acgggtcatg
     5161 ctataaccgc gtacctcgga aaactggctg gtcatcagac cattcgtgac gctattctcg
     5221 acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga aagtggtgcg gtactgatca
     5281 agcgctacgg ctttgacgca gacaaacatg cggcgtacat ccagaaaatc ctcggtcgtt
     5341 ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg ccgtcagccg ctgcgtaaac
     5401 tgagtgctgg cgaccgtctg atcaagccac tgctcggtac gctggaatac agccttccgc
     5461 acaagaatct gattcagggg attgctggtg caatgcactt ccgcagtgaa gatgatccgc
     5521 aggctcagga actggcagca ctgatcgctg acaaaggtcc gcaggcggcg ctggcacaga
     5581 tttccggtct tgatgccaac agcgaggttg tatccgaggc ggtaaccgct tataaagcaa
     5641 tgcaataatg gtggaccagg cgcaggacac cctgcgcccg aataacagat tgtcagatat
     5701 gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt gtgcttgagc gtctgaatgc
     5761 tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag cttttgaccg aggcggtaaa
     5821 tcttctggtg cttcaggtat tccgcaaaga cgattacgcg gtgaagtatg ctgtagaacc
     5881 gttactcgac ggcgacggtc cgctgggcga tctttctgtg cgtttaaaac tcatttacgg
     5941 gttgggcgtc attaaccgcc aggaatacga agatgcggag ctgctgatgg cattgcgtga
     6001 agaactaaat cacgacggca acgagtacgc ctttaccgac gacgagatcc ttggaccctt
     6061 tggtgaactg cactgcgtgg cggcgttacc accgccgcca cagtttgaac cagcagactc
     6121 cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct gtgcgatcaa caatggtcct
     6181 ttcactgact gagctgattt ccagaatcag cttaaaaaaa gcctttcaaa agtaagcaac
     6241 gtctgcttac tgcccctcta cctgcttcgg ccgataaagc cgacgataat actccagacg
     6301 ttgtagatat aacggcacgc tttcatcggg tattccggac ggaatcgcgt tacggggagg
     6361 aagttttttc agatactccc ggaacgcctg gcttgatgcc atgaaatcta cggctttatc
     6421 gataagaagc ggaacgtttt cacctaattt gcccatgatg atatctccgt attaccccgc
     6481 gccgggaatg cgcggccgcc aattttagtt taggttcccg gtaaagatga cattaaggaa
     6541 aacgtgctga atcctcaata atcatccgct gctgtttatt atcttttttc tatagatttc
     6601 ttaattaatc aacgaattgc gtagtttttg agcatgttaa tgattacgtt gcagaactat
     6661 tacaatgtca tcagttgtga acaaagcacc tggtcgcgca tactaggggc tataaattta
     6721 tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg
     6781 atcgcctgga cgccatccgc caccagcagg ccgatctatc gctggttgaa gccgcagaca
     6841 aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct cgcctgcgtg
     6901 aggttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg ccgtttcagc
     6961 gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt gttcgcggac
     7021 tggtggttgt gcacccaatg accgcgctgg gccgtgaaat gggcctgcag gagatgactg
     7081 gattctctaa gactgcgttt taagcgcctc agacgactgg ttcgatcata tcaacccgca
     7141 tctggcttgc cttagctggt caatgcgggg actctcctgt ctactcgcct aattaccaaa
     7201 caattcactc cctttacgtc gatttaccgc tggttgatat tctgaaaatg catttttacc
     7261 gaaatagcca cctataaaca ataaaaatat atagaagata tcatttatta attaacaagc
//
LOCUS       DAFNBJ010000209         7179 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000209 DAFNBJ010000000
VERSION     DAFNBJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.209"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1442..1999
                     /locus_tag="L2X49_004425"
     CDS             1442..1999
                     /locus_tag="L2X49_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799409.1"
                     /translation="MRDAEFRAMLQASRQRNKHNSYAYTNNPTSYELPKLTKKERKGI
                     DEVIRSITPRDRFMPTRKSTQNTIKNYLANFDSYEQLPSKLDDIFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINAASVTNYLQRQATRLSHELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEEF"
     gene            2000..2278
                     /locus_tag="L2X49_004426"
     CDS             2000..2278
                     /locus_tag="L2X49_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799410.1"
                     /translation="MNKHTLTRISEEAFNELTRIKRATNLPHQTVMQLAIAKEVTESD
                     LLKYPVSKGRKHIRITQDALDTLKALNGFKIDIARLLSLKIHKLAMEV"
     gene            2278..2493
                     /locus_tag="L2X49_004427"
     CDS             2278..2493
                     /locus_tag="L2X49_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799411.1"
                     /translation="MLCVTFEYHTDKMIRHISDLLIKGNGFGDIHNSKDIFIKAIGPN
                     EVLKTAVKPEWFERHKIELGYWGEEVL"
     gene            2490..2777
                     /locus_tag="L2X49_004428"
     CDS             2490..2777
                     /locus_tag="L2X49_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBN4799412.1"
                     /translation="MKPWHEDVRRYFTEHLIYDESSDSLCWSDGESVTINTDDYGNKT
                     FNIGRYTFYVKYVVWFLYHGYQSNKQIIHRNGNRADTRPKNLMQVRDFKRN"
     gene            2933..3256
                     /locus_tag="L2X49_004429"
     CDS             2933..3256
                     /locus_tag="L2X49_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799413.1"
                     /translation="MNNYPLQIFVDNDTAMMIQAFTDVGVSIDFDRLLRLMADNSEVI
                     EDFIQSVEFNEPRMMLPIIDSNMKRLVIEQTNKYSISPERYLKAAIAILYADNILVTD
                     SKVVH"
     gene            3795..4505
                     /locus_tag="L2X49_004430"
     CDS             3795..4505
                     /locus_tag="L2X49_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBN4799414.1"
                     /translation="MTNDIYINGRKVDSFGDWSPQTEHPAITIQRKEHDERIKLEQSL
                     KMSPKVISFVSPEPQEMPDICKSEALFNLEREYYPQLKAQRIKLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFPENIPDVINNLPDGYRIVKMVKASRGAG
                     QFIYMTNKSDEELCELARQNILASRNKQLENVKLYLSRELQAMKELVSEYESQKKIAM
                     QADIEQLMKISQKYAKAI"
     gene            4514..4822
                     /locus_tag="L2X49_004431"
     CDS             4514..4822
                     /locus_tag="L2X49_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799415.1"
                     /translation="MNDLTERDKRIKGVMEPFEDRFNEIKTTLGDIQEVTEYLIGRMD
                     EKPLAELKCDGRDDLTRLLIELKDKINKLDTVYYQTGLSLRYEIQKNERGVECIHYLD
                     "
     gene            4848..5051
                     /locus_tag="L2X49_004432"
     CDS             4848..5051
                     /locus_tag="L2X49_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799416.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSSTSKQNNRIRRIAK
                     WCLDKLPSDCQIVGEDGNYYLVG"
     gene            5210..>7179
                     /locus_tag="L2X49_004433"
     CDS             5210..>7179
                     /locus_tag="L2X49_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBN4799417.1"
                     /translation="MSKFTVDSFIVELGFSENVVKGLQRVEKAALQSAQRIEKNLNKG
                     FRINTKQLDSNLTSSLGQLERKFNKAFDKIEQRARSTKAFRFTSQVNDAINPPKQPRQ
                     PRISGNRAITAAYSANMSKLKGFDPVLQKYIKSQFYGLSAKAGQMDNSKFNEKLAQLN
                     ASVREAIAKAKGHTSTSINGDRAANSLDSLSSAAVKTAGAFISFQAVLSAYQKIMEVG
                     LQRASSERSIDFVFGDQSTQVKKFIQDLTQITGMDTTETQAQFASFGASAKNTMGLEG
                     SEELFKNLTGYARLMGRSDEQIQRALTAVSQMASKGQVMAEELKGQLSEALPGATQAF
                     AKALGLTEKQLFDKMKNGDVKASDALPKFAKELGSQINSRGGWKAIQDSTQTMLGNLK
                     NTWNNSLTDIFRGSENGLQDFTRSLTTLLNSLGGSGKTLGESLGNLMTSMSHGVDNLT
                     DISYRVRAFFDEVTLAYRGLTDEQKKVVDALGSGLINSLKVLAGVIAVNKVVGVTRSI
                     YGLMTAITRLGQVADTTADNVNRKNGKPSRFNLGNMLLAGSAIYAGYERLASTPERME
                     AQAKLPELAKESGLKPVFTMDDVKGWFNSAAMNLANFQHPELITMRQDAALTNTDIQS
                     LRDEISALSKRIQEPVKVSLGGEVAIKPDETS"
BASE COUNT         2385 a         1257 c         1524 g         2013 t
ORIGIN      
        1 attgtaggag catctattcc caacaaggga actacacaga acctagagag catgtctgta
       61 atgtttctgt atcaggtcaa ctataagagg tctggatatt accttgtaag attcttggta
      121 gtgattatgt agatagttag gaagaattgt ttaacgggag gaagagaacc atgaaaccga
      181 aggtaggttc tatcttaaca attatcttaa gaataaaact gaaaaatact taacagaact
      241 accaagaaga gagggaacga taagaccgga ggtagtgacc ttgttagaca ggctgtttca
      301 aagctcttaa gaatctgtta agtaaggtca tgtgaacaat cttaccattt tgtcaatgct
      361 tttgtctttg acctaaatca ataaaatgta tcgtttgatg tgcttttaaa caaagtagct
      421 aaaaaatctt tttagagggg cgcttcgctt ggggtcacta tgttctttct ctcccgttaa
      481 gaggggagtt acagggcatt tcaaagccct gataaggtaa gcccgatgat aacagaaaca
      541 gagggcaaaa ccacttatca acactgtgaa cgaataacgt aaagggctgg ttaagaaagt
      601 cggtttaggg attggtagat gattcggatg aagcatcctc ataacaatac caattagaag
      661 ggagcttccc tctctggggt tatcgctctt ctcccgctta gtggatagct aagaaggctt
      721 gaagtcatct gtgaaggtgc tgaacagcta ctatattggg tgattgcaac tcgttgaaaa
      781 ataagcattt ttagaaatca ctgttaaggt tacagagtca actgtgaaga gacttgtcag
      841 ttctctatat atatggctaa ttacaactta ttgaaaaata agaactttta gaaattactg
      901 tcagagaggt gaatacttga tagatacagc tttctggttc tgtcaagcta ttttttaata
      961 gatagcacaa aatgctaaag atacccctgt acgagatggt ataggggttt tgaagggggt
     1021 ttaacggggg tccgtgtggt cattatcctt aaggtcaagc gtccatgtac gaccgaacag
     1081 tttaaagaca acaagcactc tgacgaggac gtagcggagt aacagacctg caatcagccc
     1141 gatacctcca ccagcccata catcaggtga ccccagttca ggagggagat ttaccattaa
     1201 atttcctgtg tgcaatgcaa taccatgtga catacatagt tgattcggtt agttgggctt
     1261 gtaccaaccc ctatcgaata aacctcatag ccacgccttt atattttact ctccccatag
     1321 aaccattata ccacagatgt taataaaatg ttaaaaaata cttgacatta gtctttgaaa
     1381 atgctagtat tgtatatata agttaacaga ataataacat cttatgaggg tcggaacatg
     1441 aatgagagac gcagaattta gagcgatgct tcaggcatcg agacaacgta acaagcacaa
     1501 ctcctacgct tacacaaaca acccaaccag ctacgaacta cccaagctca ccaagaaaga
     1561 acgcaaaggc attgacgaag taattcgtag cattacgcca cgagacagat ttatgccaac
     1621 cagaaagagc acccagaaca ctattaaaaa ttatctggct aactttgatt cttacgagca
     1681 actaccaagt aagcttgatg atattttcat tggattctgt agaagtgaag gccacccgaa
     1741 atacaacaaa aaactatttt atcttctaaa gaatcttgac gaaattaacg cagccagcgt
     1801 gacaaattat cttcaaagac aagcaaccag acttagccat gaattaccaa gcgataaata
     1861 ttgcgctttg cttgctgtaa tgtgtgcaaa gctaattggt attgttgaac atcatattgc
     1921 cgttgggaat attgagccaa tggaaaacga gcaaccagat ttcgagtttg acccgtattt
     1981 agttacagag gaattttaaa tgaataaaca cacattaacc cgtatttcag aggaagcttt
     2041 taacgagctt accagaatta aacgagctac taacttgcct catcaaactg taatgcagct
     2101 tgcaattgct aaagaggtca ctgagtcgga tttacttaaa tatccagtct ctaaaggtcg
     2161 taagcacatc cgaattactc aggacgcttt agatactttg aaggcactta acggctttaa
     2221 gattgatatt gcccgtctat tgagtcttaa gattcacaag ttagcgatgg aggtttaatg
     2281 ctatgcgtta cctttgaata ccacacggat aaaatgattc gccacatctc cgacctcctg
     2341 attaagggaa acggattcgg tgacatccat aactcaaaag atattttcat taaagccatt
     2401 ggcccaaatg aagtactgaa aacagcagtt aaaccggaat ggtttgagcg tcacaagatt
     2461 gaattaggtt attggggtga ggaggtttta tgaaaccttg gcatgaagat gttagacgat
     2521 attttacaga acaccttatc tatgatgaat ccagtgattc cctgtgctgg tctgatggag
     2581 agtcagtcac gataaatacc gacgactacg gcaacaaaac atttaatata ggccgatata
     2641 ctttctacgt gaaatatgtt gtctggttcc tttatcatgg ctatcaatcc aacaagcaaa
     2701 ttatccatcg aaatggtaac agagccgata ccagaccaaa aaatcttatg caagtccgag
     2761 actttaagag aaattagaaa aatagcttct caggcgctct caatcgcatt ctaacgcaaa
     2821 aaactatctt attttagggt gggtaatacc actgtacagg ctacccctga aaaagactgt
     2881 ttagctcgtt acagagcgat tgaggaggtc tggtaaaaag gagattttaa aaatgaacaa
     2941 ttacccatta cagattttcg ttgataacga caccgctatg atgattcagg ctttcactga
     3001 tgtaggtgtt agcattgact ttgacaggct gttaaggcta atggctgata actcagaagt
     3061 aattgaagac tttattcagt cggtggagtt taacgagcct cgaatgatgc taccaattat
     3121 agacagcaac atgaaaagat tggttatcga gcaaacgaac aaatacagta ttagccctga
     3181 aagatatctc aaggcagcta tagcaatttt gtacgctgac aatattttgg tgacggattc
     3241 gaaggtggtg cattaatgga attggtgatt atctgttttg tattgattgg tatgattgca
     3301 ggtgcatcta ttaaagtata aatgttacca atctgttaaa aagctgttga caaataacct
     3361 tgagcatgac ataattgtat atatagggag agcaattctc cttgaattaa aatattaatc
     3421 gtctctacat cgatgataac tatattctga gttggaaagg tgcaatgctt ttcgtcatgg
     3481 cctttcaggt tagtctgaga ggctatctca aaaagtgctt aatcattcat tcattgaacc
     3541 ctttacagct ttgccctaat catgagttag ggcgtttctt taagagtttt caaacagaac
     3601 ttttaaagaa agtttttcct gaaacttcgt taacgttata aagcaaacct ccaaagccct
     3661 tcacagatga ggatgaacct atgtttaggg cttttcttta tagagcatct ttaataaatc
     3721 gttaagtcat cttctaagtg tattggcgtt gaaggtgctc tattaagaaa taatcacact
     3781 tagaggaaat tataatgaca aatgatattt acattaatgg cagaaaggtt gatagcttcg
     3841 gtgactggtc gccacagact gaacatccag ctatcactat tcagcgtaaa gaacacgatg
     3901 aaagaattaa gcttgagcaa tcgttaaaga tgtcaccaaa ggtaatttcc tttgtgtctc
     3961 ctgagcctca agaaatgcca gatatctgca aaagtgaagc actatttaat cttgagcgtg
     4021 aatattaccc acagttaaaa gcacagcgta ttaaacttga tgacgcttac agcaaagtta
     4081 ccctgatgca atcagctatt gagccgtcag aatttgagat tcaggatgaa ttgtcacaga
     4141 aaccatttgt ctattaccaa tacgaagata atgatggttt tggtactttt cctgagaata
     4201 tcccagacgt gattaataat cttcctgacg gatatcgaat tgtgaagatg gttaaagctt
     4261 caaggggtgc tggtcaattt atttatatga ctaataagag cgatgaagaa ctctgtgaac
     4321 ttgctagaca gaacatcttg gcatcacgta acaagcaact tgaaaatgtt aagttgtatc
     4381 tctcaagaga gcttcaggct atgaaagaac ttgttagtga atatgaatca cagaagaaga
     4441 ttgctatgca agccgatatt gagcagttaa tgaagatttc tcagaaatat gcaaaggcta
     4501 tttgaggtga ttaatgaatg atttaacaga acgcgataaa agaattaaag gggtgatgga
     4561 accttttgaa gataggttca atgaaattaa aacgactctt ggtgatattc aggaggtcac
     4621 tgaatatctt attggtcgta tggacgaaaa gcctcttgcc gaactaaagt gtgatggtcg
     4681 ggatgattta acgcgacttc ttatcgaact caaggataag ataaataagc ttgatactgt
     4741 gtattatcaa actggtcttt cactacgtta tgaaattcaa aagaatgaac gcggtgtcga
     4801 gtgtatacac tacttagatt aaaatcgaat taaaaaggag gtggtgaatg attgatttaa
     4861 cagaacgcta tgaactcatc aaggcaatta gtgaacgact gaaagaacaa cccaacaaac
     4921 caaaacgaat caaatcatcc acctctaagc aaaataacag aatcagaaga atcgctaaat
     4981 ggtgtcttga taaattacca agtgattgtc agattgttgg tgaagatggc aattattatc
     5041 tggttgggta aaaagattgg ttgggtaaaa atctacggga gagattatcg agtgacatcg
     5101 gtagtttctc ccttttttgt atgtgtgcta cgtgaaatct gtaaagctaa tacccacact
     5161 gaaaaataga atttacgtac aacacgctta aagaggtgaa atatgaacaa tgagcaaatt
     5221 tacagttgat agttttatcg ttgaattagg cttcagtgaa aacgttgtta aaggcttgca
     5281 aagagttgaa aaggcagctt tgcagtcagc acaaagaatt gaaaagaacc taaataaagg
     5341 tttcaggatt aatacaaaac agcttgatag taatttgaca agttctttag ggcaacttga
     5401 aaggaagttt aataaggctt ttgacaagat tgaacagaga gcaagaagca caaaagcttt
     5461 ccgtttcaca agtcaggtta atgacgctat taatcctcca aaacaaccaa gacagccacg
     5521 aattagcgga aacagagcaa tcacagcagc ttactcagcg aatatgagca agttaaaggg
     5581 ctttgaccct gttttgcaga aatacatcaa atctcagttt tacgggctat ctgctaaagc
     5641 tggtcagatg gataattcaa aattcaatga gaagctggct cagttaaatg cttcagtcag
     5701 agaagcaatt gctaaagcta aaggtcatac atccaccagc attaacggtg acagagcagc
     5761 taatagtctt gatagtttga gttcagcagc agttaaaaca gcaggtgcat ttatcagctt
     5821 tcaggctgta ctatccgctt atcagaagat tatggaagtt ggccttcaac gtgcatcatc
     5881 ggaaagaagt attgatttcg tattcggtga ccaaagtaca caggtcaaaa agttcattca
     5941 agatttaact caaattactg gtatggacac aaccgaaaca caagcacagt ttgcttcatt
     6001 tggtgcatct gcaaaaaata cgatgggtct tgaaggctca gaggaattat ttaaaaactt
     6061 aactggatat gcccgtttaa tgggtcgttc agatgagcag attcaaagag cattaaccgc
     6121 agtatcgcag atggcttcta agggtcaggt gatggctgaa gagcttaagg ggcaactatc
     6181 tgaagcttta ccgggtgcta ctcaggcatt tgcaaaggct ttaggtctga ctgaaaaaca
     6241 gcttttcgat aagatgaaaa atggtgatgt taaagcctct gatgcactcc ctaagtttgc
     6301 taaagaactt ggttcacaga ttaattccag aggtggctgg aaagctattc aggactctac
     6361 gcagacgatg ttagggaact taaagaatac ttggaacaac tcactaacgg atatcttcag
     6421 aggttctgaa aatggtcttc aagatttcac aagaagctta acaacgcttt taaatagtct
     6481 tggtgggagt ggtaaaacat taggtgaaag tcttggtaat ctcatgactt ctatgtcaca
     6541 cggtgtcgat aacctgactg atattagtta cagggtaagg gcgttctttg acgaggtgac
     6601 attggcttat cgtggtttaa ccgatgaaca aaagaaagtt gttgatgctt taggttctgg
     6661 cttgattaat agtttaaaag tcttggctgg tgttattgct gtaaataaag ttgttggtgt
     6721 aaccagaagt atttacgggc ttatgactgc tatcacaaga ttaggtcagg tcgctgatac
     6781 tacagcggat aacgtgaata gaaagaacgg gaaaccatca aggtttaatc ttgggaatat
     6841 gctattagct ggtagtgcta tctatgcagg ttatgaaaga ttagcctcta cacctgaacg
     6901 aatggaagca caagctaaat taccagagct ggctaaagag tctggactta agccagtatt
     6961 tactatggac gatgtgaaag gttggtttaa tagtgcagct atgaatcttg ctaacttcca
     7021 gcatccagaa ttaatcacca tgagacaaga tgcggcatta acgaatacgg atatccaaag
     7081 cctgagagat gaaattagtg ctttgagcaa acgtattcag gagcctgtga aagtttcact
     7141 tggtggtgaa gttgccatta aaccagatga aaccagctt
//
LOCUS       DAFNBJ010000210         7173 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000210 DAFNBJ010000000
VERSION     DAFNBJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.210"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(80..955)
                     /gene="pdeC"
                     /locus_tag="L2X49_004434"
                     /pseudo
     CDS             complement(80..955)
                     /gene="pdeC"
                     /locus_tag="L2X49_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            1385..1666
                     /locus_tag="L2X49_004435"
     CDS             1385..1666
                     /locus_tag="L2X49_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBN4799418.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(1765..2301)
                     /gene="ssb1"
                     /locus_tag="L2X49_004436"
     CDS             complement(1765..2301)
                     /gene="ssb1"
                     /locus_tag="L2X49_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBN4799419.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            2555..5377
                     /gene="uvrA"
                     /locus_tag="L2X49_004437"
     CDS             2555..5377
                     /gene="uvrA"
                     /locus_tag="L2X49_004437"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBN4799420.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(5412..5768)
                     /locus_tag="L2X49_004438"
     CDS             complement(5412..5768)
                     /locus_tag="L2X49_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBN4799421.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(5772..6188)
                     /locus_tag="L2X49_004439"
     CDS             complement(5772..6188)
                     /locus_tag="L2X49_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBN4799422.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(6299..7012)
                     /gene="aphA"
                     /locus_tag="L2X49_004440"
     CDS             complement(6299..7012)
                     /gene="aphA"
                     /locus_tag="L2X49_004440"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBN4799423.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1786 a         1811 c         1825 g         1751 t
ORIGIN      
        1 acatctgatg gaaatggaag ccacgcaagc accttaaaat caccatcata cactaaatca
       61 gtaagttggc agcattaccg attattggct ggtaataaag acaaagttga tgtttttcga
      121 gggcgcgttg caatttacgt ttgggagata aatagttttg tcgaatacgt agccagagta
      181 atagcagaac aagactcccc aaaataccgg cgggcaacgc aaaaataaga tgattataaa
      241 aatgggttat aagacgttga tatgaagtcg caacaatggc tgcaattggg cgttttgtcg
      301 aataaactgt cgcatataaa tagccatttc tttgtacggt taaatccttc aaatgaatca
      361 gcggagaaaa cgttgctgcc gaggcctctt tgcttaacga gaaaaaggtt ttcgtcaccg
      421 tatcatacac accccattgc aatgtcgggt catcagacat cacttcactc cagaagagag
      481 ggttgataac cgccacataa tttccccgct gcatataggt cattttatag ccagagaaaa
      541 aaggcgtatc gcggtaataa tagatagaaa cgttaggttc acgcttataa tcggccggtg
      601 caatcgtata gccgtttaca ggcgctatca gcgatgagca taaaaaatgg ttatcacggg
      661 catagatcaa ttcattaata taaagatagc cacgaataat attcaacatt cgcttttgat
      721 gggctggagt gcaaacttgc ccctgatagc gttcagctgc atcgctcact aaatctgcct
      781 ggcgaatgac cagctcggat ttatccagtg ccagttgagc aaaggttcgg agctgattat
      841 tcacttctga ttttgcccaa aggaaggcaa tccatagcga aagaatgatg ggaaagagaa
      901 ctaaaaagat aatgcccggc aacgccagta attggtgtcg tgcacgatga ctcattgcta
      961 tcaatttcct gcctgtttat acccggccat tgcaaggtga aaaccctggg taccgacgtc
     1021 tttagataaa tcttacggta taaatagcac ttttgcctga tttgaaattg atacctttct
     1081 aatcgtcacc tggatgaaaa taattcattg atattcaaaa ttaatttcct acaggaatgc
     1141 agaggcggcg ggaagatatg cccttaatgc cactgaaaaa ccacataatc tgtacagcaa
     1201 taaaagtcac ggcctaatat ggctaacaaa caatcaattg tgatatagtt cacaaaatta
     1261 atgaaacaaa cagagtgttt catttttgtg ttctgcaagc cccgcgatag ccagagtgta
     1321 ccacgcctcc cgtgaacaac gccgcgctgt ccagggcatc ggctcttttc agaggaattg
     1381 tttgatggct accctcacca ctggcgtggt tcttcttcgc tggcaacttc ttagtgccgt
     1441 aatgatgttt ctggccagca cactcaacat ccgttttcgt cggtctgatt atgtcgggct
     1501 tgcggtgatc agcagcggtc tgggcgttgt ttccgcctgt tggttcgcaa tggggttgct
     1561 tggcatcaca atggcggaca tcaccgctat ctggcacaac atcgagtcgg tgatgataga
     1621 agagatgaat cagacaccgc cacaatggcc aatgattttg acctgatacg tagaagcctc
     1681 cataacggag gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat
     1741 ataacctatt gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg
     1801 ctcgttagac ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa
     1861 ctgattgcca ccctgcggct gctgaggctg accccaaccg ccctgcggct gaccaccacc
     1921 gatattacca cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc
     1981 gccaacgttc accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt
     2041 acgggtacgc agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc
     2101 cacttctgcc agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc
     2161 gccggtcgct ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc
     2221 accatttggc atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt
     2281 gtttacgcct ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta
     2341 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
     2401 aacaaacctg gccagacatt gttacacaac actccgggta atgcattcca atactgtata
     2461 ttcattcagg tcaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca
     2521 ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
     2581 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
     2641 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
     2701 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
     2761 aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
     2821 gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg
     2881 tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc
     2941 gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat
     3001 gctgctcgcg ccaatcatta aagagcgcaa aggcgaacac accaaaacgc tggagaacct
     3061 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
     3121 gccgaaactg gaactgcaaa agaaacatac cattgaagtg gtggttgatc gcttcaaggt
     3181 gcgtgacgat cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg agctttccgg
     3241 tggtacagcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
     3301 caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccgc gactgttttc
     3361 gtttaacaac ccggcaggtg cctgcccaac ctgtgacggc cttggcgtac agcaatattt
     3421 cgatcctgac cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg
     3481 ctgggatcgc cgcaacttct attacttcca gatgctgaaa tcgctggcag atcactataa
     3541 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
     3601 cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat
     3661 ccgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacaga
     3721 atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg
     3781 cgaagggacg cgtctgcgtc gggaagcgcg ccacgtttat gtcgagaata cgccgctgcc
     3841 tgctatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactagc
     3901 tggtcagcgg gcgaagattg cggaaaaaat tcttaaagag atcggcgatc gcctgaaatt
     3961 cctcgtaaac gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg
     4021 cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat
     4081 gtatgtactg gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg
     4141 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga
     4201 cgcaattcgc gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg
     4261 tgaagtggtc gcagaaggtc cgctggaagc gattatggcg gtgcctgaat cgttgaccgg
     4321 gcagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc
     4381 agaaaaagtg ctgaagctga caggtgcacg cggcaacaac ctgaaggacg tgacgctcac
     4441 gctgccagtc ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct
     4501 gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga
     4561 accggcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga
     4621 ccaaagccca attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac
     4681 acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg
     4741 acgtttcagc tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat
     4801 caaagtggag atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa
     4861 acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct
     4921 ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa
     4981 gctgcaaacg ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac
     5041 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac
     5101 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
     5161 gcaactcctc gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga
     5221 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg
     5281 cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc
     5341 ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg
     5401 gccttttcct ttcagagttg taccagcaat ttacgctttt cttccggtag taaattcact
     5461 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg
     5521 agatacacgg tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc
     5581 tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt
     5641 tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag
     5701 tcgttatgca cgctctgttc tgcgcctggt tttgccatgc aatattgtag caactccgaa
     5761 atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg
     5821 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgtaac
     5881 ggcaatacaa gcgatgttcc cagcattgag gatttgatat gagaaggcat atcgtctgct
     5941 ccctcatagt catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacgctcc
     6001 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
     6061 tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tatcagccag ctgattcaga
     6121 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
     6181 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa
     6241 ggataaaggt gtgattaaaa aaacagcatt cgggagagtg tcacgctctc ccgctctgtc
     6301 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
     6361 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcagcgg
     6421 taatatcgtt atcggaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgcg
     6481 attttgtatt ttgccctggt ttatcgcccg caaagatcac cggattcatg ttggtgacag
     6541 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc gggctacgac
     6601 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
     6661 cctcttttgg aatgctgaat tcatcccagc cattgttcat tttttcccag aacacaggat
     6721 ttttcagata atcttcgctt tctggtgaga aggttttttt gccgcgccag aagcccgggc
     6781 tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga
     6841 ggctattttc aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg
     6901 caacgttagt cccagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
     6961 ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc
     7021 ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc
     7081 ataacgccag ctgccgggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga
     7141 tgattatgtt taacccagta aatacgttcc tgt
//
LOCUS       DAFNBJ010000211         7173 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000211 DAFNBJ010000000
VERSION     DAFNBJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.211"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            23..154
                     /locus_tag="L2X49_004441"
     ncRNA           23..154
                     /ncRNA_class="other"
                     /locus_tag="L2X49_004441"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(338..1015)
                     /gene="narI"
                     /locus_tag="L2X49_004442"
     CDS             complement(338..1015)
                     /gene="narI"
                     /locus_tag="L2X49_004442"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBN4799424.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1015..1725)
                     /gene="narJ"
                     /locus_tag="L2X49_004443"
     CDS             complement(1015..1725)
                     /gene="narJ"
                     /locus_tag="L2X49_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBN4799425.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1722..3260)
                     /gene="narH"
                     /locus_tag="L2X49_004444"
     CDS             complement(1722..3260)
                     /gene="narH"
                     /locus_tag="L2X49_004444"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBN4799426.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3257..7000)
                     /locus_tag="L2X49_004445"
     CDS             complement(3257..7000)
                     /locus_tag="L2X49_004445"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBN4799427.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAAEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         1589 a         2035 c         1877 g         1672 t
ORIGIN      
        1 tcaagcgaat ccgcaattaa atattctgcc catgcgggga aggatgagaa gcttcgacta
       61 aggttcgact tgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag
      121 cgcgcagcgc tgagtaatcc ttcccccacc accatcgctt ccttaaataa tcaaaacctg
      181 ttactccgag ttaattttgc ccgatggcac cacgtttacc gggcctacaa tatcctcccc
      241 tgtaggtcgg ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa
      301 ccccgccgaa gcagggtcta tgtgaactaa aattcgctta gtgacgagcg cgcaccagct
      361 ggtacttacg tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg
      421 ggaacagcag gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac
      481 catcgaggtg ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca
      541 gtttcatcat ctcgctaccg tccatatgct gagcggagaa cggaatggtc aacaggccca
      601 gcgcgcactg gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac
      661 gcacgcgtgg gctgaacaga cgacgcttca gcaacagcac gccgccaatc agacacagca
      721 cgccgctggc accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg
      781 cttcatacat ccagtgcggc gtcagcatac cgaagaagtg accaacaaaa atccccagaa
      841 taccgatatg gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg
      901 acgccgcgcg ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga
      961 ccgccccggc tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg
     1021 ctgtcctccg gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg
     1081 atgagcagtg attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc
     1141 ttcccagaca gcatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc
     1201 cgccactttg tcgctgtcga tagcggtatt cgccagtttc agcagcagat caaacagcac
     1261 ggcataacgg ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat
     1321 atctttcaaa ccttccacgg cttcgctttg cggcagctgc gccaggtact ccagatacag
     1381 cggcagatgg tccggcaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc
     1441 cagcaggtcc accatcgcct ggccgcggtc gcgggattcg ccgtgcacat gttcaaacag
     1501 caacagtgac gtggcgcggc cacggtcgaa cagttcgctg tactgggcct gggcatcgag
     1561 cggatccatc gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cctcttttgg
     1621 cagatttttc gacgcggcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc
     1681 atccggatat tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt
     1741 ttgctggtca catcgatggc atcgatacga cggctgttga acagattgaa tttggtatct
     1801 gaaccgtggc agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc
     1861 cgtgccagtt cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc
     1921 agataacggt acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg
     1981 gtatcaactt taccgtcaac ggtttcagca cgtttgtaat gacgcatcgc cagcatacgt
     2041 ttcagcgcgc gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt
     2101 accggaatac gcaggctttc gacgtctggc agaatgccgt tgctacccag ctcacccgcg
     2161 tctgctgcag actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga
     2221 tattctggat gcagcggcag cgccagtttc cattccattg ccattttata aaccggcgac
     2281 tgctgtgcag cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact
     2341 ttcggatcgt tcggatcgag gaacacgtcc agctgacgct ggtaaagatc tttctcgttc
     2401 tcggtgctgg ctgcacgttc aatcgcgtcg gcatcgtata acagcacgcc gagataacgg
     2461 atacgaccga cacaagtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag
     2521 cagaagatgc acttctcaga cttaccgctc ttccagttga agtagatttt tttgtatggg
     2581 catccggtga tgcacatacg ccagccacgg catttatcct gatcgatcag gacgatgcca
     2641 tcttcttcac gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg
     2701 cagtgttcgc acaggcgcgg caaatacatc atgaaggtgt tttcgaactg gctatacatc
     2761 gccttctgga tgttgtcgaa gttcttgtct ttcgccagtt tgtcaaactc accacccagg
     2821 tcatcttccc agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa
     2881 cgcggacggg caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgataa
     2941 tcaaaatcga acggctcgta ataatcgtcg atccccggca gatgcgggtt agcgaagatt
     3001 ttacccagca gcatggcgcg gttacccatg cgcggctgca gtttgccatt gattttacgg
     3061 atccagccgc ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctgacccggc
     3121 ttggtttcca cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt
     3181 ttacaggtaa ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg
     3241 acttgtgaac gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta
     3301 accagtcaat gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg
     3361 tgccgtagta gttaaagccg tatgccagat gggcatagcc gccgatcata tgcgtcggtt
     3421 tcggcgtgat acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac
     3481 caggcaggtt aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac
     3541 gctggctgac aaccgcacgg gcagtcagag caccgttgct gttgaatact tcaatccagt
     3601 cgttatcggc gatacccaga tctttggcat cggcttcact caaccagacc accggaccac
     3661 cgcgacctaa agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct
     3721 ggtgcggcgt caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca
     3781 cttctttcac cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat
     3841 cacgcatcca ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga
     3901 tcagctcgtg aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag
     3961 accaggtcgg gctggagata attttgcgcg gctgtgcctg aatatcgcgg aagcggatct
     4021 tctcgtcttc tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg
     4081 cagcccaggc tttcacggct acctgaccgt tggtttccgg tgccagtgtc aggatcatct
     4141 ctgccgcatc aattgcggtg ttcagcatcg gctggccttt cgccggacct tccgctttgg
     4201 tgtagttgag cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac
     4261 cgccattacc gattttctcc atcagcgggc cgatagaggt aaagcgttcg taagtcgccg
     4321 gataatcgcg ctctacgacc ataatgtgtg gcgcggtttt acctgggatc aggtcgcact
     4381 cgcctttttt ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcatgct
     4441 ggataggcag cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga
     4501 atttcttcgc gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga
     4561 ccgccgcaga cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt
     4621 cgtaccaggt cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga
     4681 agtccagcgt aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt
     4741 cttccggctt cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc
     4801 ccagcaggta cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac
     4861 gccagatgaa caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac
     4921 ggatggaacc ctctttcagg gatttcaccg tatagtccac cggattcatc ccggcttttt
     4981 ccgcttcggc agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc
     5041 ccatgcgttc cgcacggacg ttaaagtcga tcaggtgtcc ggtataacgg gatttatccg
     5101 ccatcggtga cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt
     5161 tatagaaata agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg
     5221 ccagcggctg ccagccggtt tgtggacgca gtttttcctg acctacatag tgcgcccagc
     5281 cgcccccgct ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccgcgatagt
     5341 tcatgtccag gtgataccag tgattcagcc ccgcaccgac gataatcatc gaacgaccgt
     5401 gcgttttatc agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa
     5461 cgccggtaat ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg
     5521 cacagttaac gtcgttcaga ccacgttcca gaccgtagtt tgccagcgtc agatcataaa
     5581 cggtggtcac cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt
     5641 gcagcagcac gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt
     5701 acgggaagcc tacctcagcg atctcatcct ggctacccag caggctcagt tgcaactcgg
     5761 tttcttcgcc agttttgcca tcgcgctgct caagattcca tttgcccttc tcgccccagc
     5821 ggaagccaat agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt
     5881 tccattccgg attgttttcc tggcccagcg catcaaccag atcagcagcg cgcagcatac
     5941 gacctgcagc gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt
     6001 agcgacgcac atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca
     6061 ttacgtggcc catcgccagc gccattgctg catcggtgcc ctgtttcggt gccagccaca
     6121 gatcgcacag tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt
     6181 tgtaacgcac ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgagc
     6241 cccaggcgat gatgtagctg gagttgtacc agtcagcaga ttccggtacg tcagtttgct
     6301 cgccccaggt ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc
     6361 aagtaccgcc aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg
     6421 ccggaattgg cgagaaacca gcaacacggt ccgggccgta gtttttgatg gtgtaaacgt
     6481 tagatgcggc gatcagttcg ttcacctcct gccaggaaga acgaacaaaa ccaccgcgtc
     6541 cacgcgcctg cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct
     6601 caaccggatc gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca
     6661 tcatcgggta tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg
     6721 ggcagccgcg aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct
     6781 gggtttccca ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc
     6841 agtttacccc gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat
     6901 cctcccagtc acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct
     6961 tctgtttgaa gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc
     7021 ctgtcggccc tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa
     7081 cagcgtggga attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa
     7141 gatacccctt taggataatt aacagccaca gca
//
LOCUS       DAFNBJ010000212         7145 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000212 DAFNBJ010000000
VERSION     DAFNBJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.212"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(114..371)
                     /locus_tag="L2X49_004446"
                     /pseudo
     CDS             complement(114..371)
                     /locus_tag="L2X49_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611293.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            595..2142
                     /locus_tag="L2X49_004447"
     CDS             595..2142
                     /locus_tag="L2X49_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBN4799428.1"
                     /translation="MARKPKEIKTDPLEVILWKAADKLRKNIDAAEYKHVVLGLIFLK
                     YISDSFESHYELLKAGKGEFAGADPEDKDEYTAYNVFFVPELARWNYLISKAKLPEIG
                     KMVDDAMELIEAGNPQLKGVLPKVYARQNLDATVLGELIDLIGNIALGDAKARSADVL
                     GHVFEYFLGEFALAEGKQGGQFYTPKSIVSLLVNMLEPYKGRVFDPCCGSGGMFVQSE
                     KFVEAHQGNIDDISIYGQESNQTTWRLAKMNLAIRGINSEHVRWNNEGSFLNDAHKDL
                     KSDFIIANPPFNVSDWSGEQLRGDARWQYGIPPAGNANFAWMQHFLYHLSPKGQAGVV
                     LAKGALTSKSSGEGDIRAALVKDANVIDCIVNLPAKLFLNTQIPAALWFMRRDRHNSN
                     QYRDRSKEILFIDARNLGHLINRRTKVLSDDDIKTIADTYHNWRNKGGDYEDVAGFCA
                     SVDIAEVAKLDYVLTPGRYVGLADEEDDFDFKERFTALKAEFEAQLEEEARLNRAIVD
                     NLAKVVL"
     gene            2139..3332
                     /locus_tag="L2X49_004448"
     CDS             2139..3332
                     /locus_tag="L2X49_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBN4799429.1"
                     /translation="MSRWRNFCITELADFCNAQRIPLSAMERAHKKGNFPYYGASGIV
                     DYIDNYIFDGSYILISEDGENLKSRKTPIAFEATGKFWVNNHAHVLKAKKPHLTALII
                     QYFSQLDLSPYLTGAAQPKLNKASLNLIPLYLPIDESEQKAIASILSSLKDKIDLLHR
                     QNKTLESMAETLFRQWFIEETHADWEITTLDCHITVAKGLSYKGAGLTTSDNGIPLFS
                     LNSVLEGGGYKSAGIKYYNGDFKERHIIKPGDIIVANTEQGHEYRLIGYPAIIPKTES
                     ELSIYTHHLFKISINDDSYLTNAFLYYLLCSKDMHEQVVAATNGSTVNQLSADGLQRP
                     EFKLPPECMVKEFTTQIDFFWKKISINNSQIKNIESLRDTLLPKLMSGEVRVQYAEEA
                     IASVA"
     gene            3391..6630
                     /locus_tag="L2X49_004449"
     CDS             3391..6630
                     /locus_tag="L2X49_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBN4799430.1"
                     /translation="MAKMTESDIEVMAIEHLQGLGYEYVYGPDIEPSGINPLRSYQQV
                     ILEDKVRTALQRINPHLSEQKCEEALKQVMQISSPDLMANNLTFHRLLTEGINIEVSK
                     DGNTQGELASLIDFNDPTNNAFLVINQLTIKEGNHTRRPDLILFINGLPLVVIELKNA
                     ADENATVTGAYNQIKTYQNQIPGLFNYNAFNVISDGLEAKAGTVSADFSRYMTWKTAN
                     GKTQATSTQPQLEVLLQGLLNPVTLLDIIRHFIVFEASKHEDSKGIISIRTVKKMAAY
                     HQYYAVNAAVLSTIRASAVNADSPSAEVALRQQGRNSKDLVNAQKTGDRKAGVVWHTQ
                     GSGKSLSMVFYTGKIVLALDNPTVVVITDRNDLDDQLFGTFSSATQLLRQTPKQANNR
                     EELKEYLRVASGGVVFTTIQKFQPDDGSNIYELLSDRTNIVVIADEAHRSQYGFSAKE
                     VDVKDSEGNVTGKRTVYGFAKYMRDALPNATYLGFTGTPIEKTDVNTPAVFGNYVDIY
                     DISQAVEDGATVRIFYESRLAKIAISDEGRQLIEDFDDEFNEDELTLTQKERSKWARI
                     EGLIGSSKRIKAIAADMVLHFEQRLKSNADHGKGMIVTMSRRIAAELYKEIIALKPEW
                     HSDDLNDGIIKVVMTSSAADGPEIAKHHTTKKERQVLANRMKDDDDKLKLVIVRDMWL
                     TGFDAPSMHTLYIDKPMKGHNLMQAIARVNRVYKDKIGGLVVDYLGIASDLKEALSFY
                     SDAGGRGDPAEVQEEAVTLMQEKLEILEGMMHGYDYKAYFAATTSQRLTIILESENHI
                     LGLDNGKGKMRFLAAVAALSQAFALATPHDKAMEAAPEVAFFQAVKARLNKFTENSDG
                     SEEEHNDSLEVRVKQTIDQALVTDKVVDIFDAAGIQKPDISVLSEEFLQEMKDYQHRN
                     IALETLKKLLSDEIKVRSNQSITQGKKLIDMLTSAINGYQNKVLTAAEVIDELIKLAK
                     TIQESDSLASQLNLSAYEYAFYSAVADNDSARELMEKEKLRELAVVLTEAIRNNVSLD
                     WTVKEAARAKIRVVVKRLLKKYGYPPDMSLLATETVLKQAELLAGELGK"
     gene            complement(6676..>7145)
                     /locus_tag="L2X49_004450"
     CDS             complement(6676..>7145)
                     /locus_tag="L2X49_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBN4799431.1"
                     /translation="QHDFTSLKKLRPQSATSRIQPGSDIIVCAEMDEQWGYVGAKSRQ
                     RWLFYAYDRLWKTVVAHVFGERTIATLERLMSLLSPFDVVIWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2098 a         1585 c         1588 g         1874 t
ORIGIN      
        1 cattaaaaat tacaactatg gtttgtcata gcctgtacat ctattcacct catgcatatg
       61 cactaagaaa gcttttttga atactggagt tgtccctgct aaaacggaca cggctaatta
      121 actgctaata tggcatcacg atattcgcgt ggcgatttca tgttcaaccc cttgtgtgga
      181 tgcagattat tataatcctc tatctattct gccattgttt ccatcattta gactgcatcc
      241 agcagatcat ctacgtacac ataatcccgc ttgaacgtct ttacaaacga ttctgccatg
      301 ccgttacttc ccggactgcg tactggtgta gtacacacga tgaaccacag tgaactagcg
      361 aatgtgcgcg tggcatcggc tgtgcagcaa ttatgccaat gacactggct ccattgacgg
      421 caatgacctg acatctacaa tcccgctaca aagcatttac tccatgagtc attgcacttg
      481 ctgagctaca cgaacacact aaaaccagat aataattaat cgctgtataa gctcgtaaca
      541 agcggaactt taaggcaaaa tagggccatc ttcccccttc ataaggttac gctaatggcc
      601 agaaaaccta aagaaatcaa aacagacccg ttagaggtca tcctgtggaa agcggcagac
      661 aaactgcgta aaaacattga tgcagccgag tacaagcacg tcgtactagg cctcattttc
      721 cttaagtaca tttctgattc ttttgaatct cattatgagt tgctgaaagc cggtaaaggc
      781 gagttcgcag gtgctgatcc ggaagacaaa gacgagtaca ccgcttacaa cgttttcttt
      841 gtccctgagc ttgcacgttg gaactatctg atttctaagg ccaagcttcc tgaaatcggt
      901 aagatggttg atgatgcgat ggagcttatc gaagcgggca atccacagtt aaaaggtgtg
      961 ttgccgaaag tctatgcccg acaaaacctc gacgccactg ttctgggtga gctgatagat
     1021 ctgattggca acattgcact aggagatgcc aaagcacgct ctgctgacgt tttaggtcac
     1081 gtattcgaat acttcctagg tgaatttgca ctggcagaag gcaaacaagg cggccagttc
     1141 tatacgccaa aatctattgt aagcctgctg gttaacatgc tggaacccta taaaggccgt
     1201 gtctttgatc cctgctgtgg ttctggtggt atgttcgttc agtcagaaaa atttgtagaa
     1261 gcacatcagg gaaatattga cgatatttcg atctatgggc aggagtccaa ccagaccact
     1321 tggcgtctgg caaaaatgaa cctggcaatt cgtgggatta attctgaaca cgttcgctgg
     1381 aataatgaag gttcatttct taacgatgct cacaaagatt tgaaatctga ttttatcata
     1441 gctaacccac cgtttaacgt ttccgactgg tctggtgagc agcttcgtgg tgatgcccgc
     1501 tggcaatatg gcattccacc tgctggcaac gctaactttg catggatgca acattttttg
     1561 tatcacttgt ctcctaaagg gcaagcgggt gttgttctgg caaaaggggc attaacttct
     1621 aaaagctctg gtgaaggtga tattcgtgct gcactggtaa aagatgccaa tgtgattgac
     1681 tgtatcgtca acttaccggc aaagctgttc ctgaatactc agatccctgc tgcattatgg
     1741 tttatgcgcc gtgaccgtca taacagcaac cagtatcgtg atcgtagtaa agaaattctg
     1801 tttattgatg cccgtaatct cggtcattta attaatcgtc gtaccaaagt actttctgac
     1861 gacgatataa aaaccatcgc tgatacctat cataactggc gtaacaaagg tggcgactac
     1921 gaagacgtcg ctggtttctg tgcgtcagta gacattgctg aagttgctaa actcgattat
     1981 gtgctgacac ctggccgtta tgttggtctt gctgatgaag aagacgattt cgactttaaa
     2041 gaacgcttta cggctcttaa agctgagttt gaagcgcaac tggaagaaga ggcgcgtctg
     2101 aacagagcca tcgttgataa tctggcgaag gtggttttat gagtaggtgg agaaattttt
     2161 gtataactga attggcagat ttttgcaacg cacaaagaat tcctttgagt gctatggaac
     2221 gagcccataa gaaaggaaat tttccatatt atggagcgtc aggaattgtc gattatatcg
     2281 acaattatat ttttgatggc agctacatcc ttatttctga agatggagaa aacttaaagt
     2341 ccagaaaaac acctatagca tttgaagcta ctggaaaatt ttgggttaat aatcacgctc
     2401 atgtccttaa agctaaaaag ccccatttaa cggcattgat aatccaatat ttttcacaac
     2461 ttgatctatc tccttattta acaggtgctg ctcaaccaaa attaaataaa gcatcgctaa
     2521 atttgattcc tctttactta ccaattgatg aatctgaaca aaaagccatc gcctctattt
     2581 tgtcctctct taaagacaaa atagacctgc ttcatcgcca gaataaaact ctggaatcca
     2641 tggcagaaac cctatttaga cagtggttta ttgaagagac gcatgcggat tgggaaataa
     2701 cgactcttga ttgtcatatt acagtagcta agggattaag ctacaaggga gccggcctta
     2761 ccacgtcaga taacggcatt ccacttttta gtttgaactc agtacttgag ggcggtggtt
     2821 ataagagtgc aggtataaaa tactacaatg gtgattttaa agaaaggcat attataaagc
     2881 ctggggatat cattgtagcc aatacagaac agggccatga atatcgactc atagggtatc
     2941 ctgctataat tccaaaaaca gaaagcgaat tatccatata tacgcatcat ttatttaaaa
     3001 tcagcatcaa tgacgatagt tacttaacca atgcttttct gtactattta ctttgctcca
     3061 aagatatgca tgagcaagtt gttgctgcaa ctaacggttc aactgtaaat cagctgtcgg
     3121 ctgacggatt acaacgccca gaattcaaac tccccccgga gtgtatggtg aaagaattca
     3181 ctacacagat tgatttcttc tggaaaaaaa taagtattaa caactcacaa attaaaaaca
     3241 tagaatctct gcgcgacact ctccttccca aactgatgag cggcgaagta cgggttcagt
     3301 atgcggaaga agcaatcgca tcagtagcat aaaataatta tgcgggtgta cagcccgctc
     3361 tctggttaag cggcaataag gacagtacgc atggcaaaga tgaccgaatc cgatattgaa
     3421 gtaatggcaa ttgagcacct gcaagggctg ggctatgagt atgtttacgg cccggacatt
     3481 gaacccagtg gaatcaatcc gttacgtagc tatcagcagg ttatccttga agataaagtg
     3541 cgtacagcat tgcaacgaat taacccgcac cttagtgagc agaagtgtga agaagctcta
     3601 aaacaggtga tgcagatcag ctcacctgac ctaatggcaa acaatctaac ctttcatcgc
     3661 cttttgaccg aaggaatcaa tatcgaagtc agcaaggatg gtaatacaca aggagaactg
     3721 gccagcctga tcgactttaa cgatcccact aataatgcat ttctggtcat taatcagctc
     3781 accatcaaag aaggcaacca tacccgccga cctgatctta ttttattcat caatggcctg
     3841 ccattagtcg ttatcgaact taaaaatgcc gctgacgaaa atgcaacggt aacaggtgcc
     3901 tataatcaga ttaaaaccta tcagaaccaa atccccggtc tgtttaacta caatgctttt
     3961 aatgtgatat ctgacgggct ggaagccaaa gcggggacgg tttctgccga tttcagtcgt
     4021 tatatgacgt ggaaaactgc taacggtaaa acgcaagcca ccagtaccca accacagctt
     4081 gaagttttat tacaggggtt gcttaatcct gtaacgctgc tggatataat ccgccacttt
     4141 atcgtgtttg aggccagcaa acatgaagac agcaaaggga ttatcagtat ccgtactgtt
     4201 aaaaaaatgg cggcttatca ccagtactac gcggtcaatg cagcagttct ttccactatt
     4261 cgtgcctcag cggtgaatgc ggactccccc tctgccgaag tggcactgcg ccagcaggga
     4321 cgtaacagta aagatcttgt taatgcgcaa aaaaccggag atcgcaaagc gggcgtagtc
     4381 tggcatactc agggttccgg taaatcgctt tcgatggtgt tttataccgg gaaaattgtg
     4441 ctggcgctgg ataatccgac agttgttgtg attactgacc gtaacgactt agatgatcag
     4501 ctattcggta cgttctcttc cgcgacccag ctacttcgtc agacaccaaa acaggccaac
     4561 aaccgggaag aactcaaaga atatttgcgt gtcgcctctg gcggtgtggt gtttactact
     4621 attcaaaaat tccagcctga tgatggcagc aatatctatg agttgttgtc agacagaacc
     4681 aatattgtcg ttatcgctga tgaagcacac cgttcccagt acggtttcag cgccaaagaa
     4741 gttgacgtga aagacagcga aggcaacgta acaggtaaac gcaccgttta cggctttgcc
     4801 aaatatatgc gtgatgcctt acctaatgcg acctatctcg gctttaccgg aacccccata
     4861 gaaaaaacgg acgtcaacac gcctgctgtt tttggtaact atgttgatat ctacgatatc
     4921 tcgcaggccg ttgaagatgg tgcaacagtt cgtatctttt atgaaagccg tcttgccaaa
     4981 attgccatca gtgatgaagg tcgtcagctt attgaagact ttgatgatga gtttaacgag
     5041 gacgaactga cgctcacgca aaaagaacgt tctaaatggg ccagaatcga aggtttgatt
     5101 ggcagttcaa aacgtattaa agcgattgcg gcggatatgg ttctgcactt tgagcagcgc
     5161 ttaaaatcca atgccgatca tggtaagggc atgattgtta ccatgtcccg ccgtattgct
     5221 gctgaactat ataaagaaat catagcctta aaacctgaat ggcacagcga tgatttaaat
     5281 gacggtataa taaaagtcgt catgacctct tctgctgctg acgggccaga aattgccaaa
     5341 caccacacca caaaaaaaga acgtcaggtt ctggctaacc gtatgaagga tgacgacgac
     5401 aagctgaaac tggtgatagt gcgtgatatg tggttaaccg gcttcgacgc tcccagcatg
     5461 catacgctgt atatcgacaa accaatgaaa ggccacaacc ttatgcaggc aattgcccgt
     5521 gtgaaccgtg tgtataaaga taagataggc ggtctggttg ttgactatct gggcattgcc
     5581 tctgatttaa aagaagccct ctccttttac tctgatgcag gtggacgtgg agatcctgct
     5641 gaggttcagg aagaagccgt aacgctcatg caggaaaagc tggaaatcct ggaaggcatg
     5701 atgcatggat acgattacaa agcttacttt gccgcaacta cctcacaacg cctgacaatt
     5761 attcttgaat cagaaaacca tattttaggg ctggataacg gtaaaggtaa aatgcgtttc
     5821 ctcgctgcgg ttgcagcctt atcgcaggca tttgcattgg cgacaccgca cgataaagca
     5881 atggaagcgg cacccgaagt agcattcttc caggcagtaa aagccagact gaataaattt
     5941 actgaaaact cagacggatc agaagaagaa cacaatgaca gtctcgaagt tcgggtaaaa
     6001 cagactatcg atcaggctct ggttaccgat aaagttgttg atatttttga cgctgctggg
     6061 atacaaaaac ctgatatttc cgttctttct gaagaattcc ttcaggaaat gaaggattac
     6121 caacacagaa atattgcttt ggaaacgctt aaaaaactgc tttctgacga gatcaaggtt
     6181 cgctcgaatc aaagtatcac ccaaggcaaa aaactgattg atatgctgac ctctgcaatc
     6241 aatggctacc agaacaaggt actgaccgca gcggaggtaa ttgatgagct aatcaagctt
     6301 gccaagacta tccaggaatc tgacagcctt gccagccagt taaacctcag cgcttatgaa
     6361 tatgccttct attctgctgt tgcagataac gacagtgctc gcgagttaat ggaaaaggaa
     6421 aaactacgag aactggcagt cgtacttacg gaggctatcc gcaacaatgt cagtcttgac
     6481 tggacagtga aagaagcagc aagagcaaaa attcgcgtgg tggtaaaacg tctgctcaaa
     6541 aaatatggtt atccgcctga tatgtcattg ctcgccacag agactgtttt gaagcaggct
     6601 gaacttttag ctggagaatt aggaaaatga ttccctaaat ttttagcata taaactattt
     6661 ggtaatggtc ccaacttatt gatagtgttt tatgttcaga taatgcccga ttactttgtc
     6721 atgcagctcc accgattttg agaacgacag cgacttccgt cccagccgtg ccagatgctg
     6781 cctcagattc aggttatgcc gctcaattcg ctgcgtatat cgcttgctga ttacatgcag
     6841 ctttcccttc aggcgggatt catacagcgg ccagccatcc gtcatccata tcaccacgtc
     6901 aaagggtgac agcaggctca taagacgctc cagcgtcgct atagtgcgtt caccgaatac
     6961 gtgcgcaaca accgtcttcc agagcctgtc atacgcgtaa aacagccagc gctgccgcga
     7021 tttagccccg acgtagcccc actgttcgtc catttccgcg cagacgatga tgtcactgcc
     7081 cggctgtatg cgcgaggttg ccgactgcgg cctgagtttt ttaagtgacg taaaatcgtg
     7141 ttgag
//
LOCUS       DAFNBJ010000213         7071 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000213 DAFNBJ010000000
VERSION     DAFNBJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.213"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(56..4276)
                     /locus_tag="L2X49_004451"
     CDS             complement(56..4276)
                     /locus_tag="L2X49_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBN4799432.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGH
                     RVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEW
                     LTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQS
                     QHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDD
                     ERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEVTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEK
                     LQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRVWLAQCGLTVEQLARQVEP
                     EYTPARKVHFYHCDHRGLPLALISEDGNTVWSAEYDEWGNQLNEENPYYLYQPYRLPG
                     QQYDEESGLDYNRHRYYDPLQGRYITQDPIGLAGGWSLYAYPLNPVQHVDPLGLSTMI
                     IGNGPVPDNPFGHAAAANRYGLMSSGTGDEMGASVSDYFKKMQPRRDTWIWIIDTTDE
                     EEQCMMNKAIDLNKKLKTINLGPLPVANNCFSRTNMIFEACGFSNPSMNTNAPISLQV
                     LGELYGYQRYFMPKGPFTGFPFEFQEVK"
     gene            complement(4344..6452)
                     /locus_tag="L2X49_004452"
     CDS             complement(4344..6452)
                     /locus_tag="L2X49_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBN4799433.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT         1376 a         2179 c         1844 g         1672 t
ORIGIN      
        1 aagatataat tatcatggaa ataattatca ctaatatctt cccaagcatt tccatttact
       61 ttacctcttg aaattcaaat gggaatccag tgaaaggacc tttaggcata aaatatcttt
      121 gatatccata taactcaccg agcacttgta ggcttatagg cgcatttgta ttcatagagg
      181 ggttgctaaa cccgcacgcc tcaaaaatca tgttcgtccg gctaaaacaa ttatttgcaa
      241 caggaagagg cccgaggtta atagttttta gttttttatt aaggtctatt gccttattca
      301 tcatacattg ttcttcttca tctgttgtat ctattatcca aatccaggta tctctgcggg
      361 gttgcatctt tttaaaataa tcagagacag aggctcccat ctcatcccca gttcccgaac
      421 tcattaatcc atatcgattt gccgccgcag catgcccaaa aggattatca ggaacggggc
      481 cgttacctat tatcatagta ctaagcccta atggatcaac gtgttgcact ggattcagcg
      541 gatacgcata cagactccat ccccccgcca gcccaatcgg gtcctgggtg atataccgcc
      601 cctgcaacgg atcgtagtac cggtgacggt tataatccag ccccgactcc tcatcgtact
      661 gctgccctgg cagacggtat ggctgataca ggtaatacgg gttctcttca ttcagctggt
      721 tgccccattc atcgtattct gcactccaca cagtattgcc gtcttcgctg atgagcgcca
      781 gcggcaggcc ccggtggtcg cagtggtaaa aatgaacttt tcgcgccggt gtgtattccg
      841 gctccacctg tctggccagt tgctccaccg tcagcccgca ctgcgcaagc cacacccggc
      901 tttcactgct cacgcggtct gcccggattt cttcctccag tctgtccagc agccgcacca
      961 gttcagccgg aaataccacg ccgtgcccgt cctcgctccc ttcctgctgc agcttctccg
     1021 ccaggctgcg gcgctgcgct ttctcccgct caccgttgtc tgtttcgatg cggatgagcg
     1081 gcgcgaagct ccccggctga tataccgtct ggatacgcgt ggtgtcggtc tgtaccgtcg
     1141 tcagccggtc gccgtcccac ccgtaccacg tcacctccgg tttacgcgac agcgacatcc
     1201 accccgtcag gtcacgctcc cgccgccata cccgtttcgc cattcgccgt cccagcgggt
     1261 cgtagaggta gcggctctcg accagtggct cgccatgctg tatccgcgtg tagaacacca
     1321 gacggtgctg gctgtcgtag tggtagtggt gggtccgctc gtcgtccgtc cggataacac
     1381 ccgccgggat aaggtccgtc ttctccgtca gtctgccgta ttcatcatag tggtaaacat
     1441 agtgcgcatc ctctgcgatg cggttatccg gccacgccgt gagggtactg tccgggtgca
     1501 gctccgggtc cggcaggcgg tttcccgccg ggtcggtggc atacgggatg cggatgtcca
     1561 ggtccggtgc gagggtacgc acgctctcca gcctgcccgt ggcgctgtag ccgtattccc
     1621 gcgtctgtcg cgggccgctg atgcgcacca ggtcgccgtt gtcattccac ccgtagtcac
     1681 ggtcatacac caggctgttc aggtgctggc tctgtaactg gcctgcgggg gtgtatgtgc
     1741 tggtcagttt atatgcggca ttactgcctg ccatgctgcc aaagctgcgc accgtctccc
     1801 ggtgcatgcg gtcgcgcgtg tactccagca gcggcgtgtc gccaagcttc atgcccgcca
     1861 gataaccgct gccgtaggtc agccactcca ccggcggcag gctgtccggc gtgacgcggt
     1921 ttgccagccc ctgttcgttg taggcgtgtg ttgtctcatg ctgccacagc agttcccccg
     1981 tctccgggtt ctccaccgtc tgccgttcgc cggtcaggcg gcccttatcg tcgtagccgt
     2041 agtggacagc gacacggtgg ccttcgctca ggtggctgat gtctgtcagc cagccgtggt
     2101 catcatactg ccactgctct gccggttcgc cgttcaccgt gcggtgagtg atacggtccg
     2161 attcatcgta gtaccagagg gtgacaagcc cctcatcttc actctgcgtg agttttccgg
     2221 tcaggtcata atgataacgt tgcgtccgcc cgtcaaagcc gccctgctgt accaaccggt
     2281 ccagcgcatc gtagctgaag tcgctgtggc tgccgttctc gttagtcaga ctgatgacac
     2341 gtccggcagc atcgtactcc atactgcgcg tcagcccgcc ctgcgtggtg ctgaccgcct
     2401 ttccccacgc atcgtactgt gtctcgctcc ggttgccgtc cggggtgata acggcagtca
     2461 ggtcgcctgc ggcgttgtac tcatactgcg tttcacggcc ctgtgcgtct ttcaccgagg
     2521 ttaaccggcc acggttgtca tagtggcggt aaaggctgat accttcctcg cggtgaaccg
     2581 ccgtcatctg gccgaagcgg tcgtattcat agcgggtctg gtagcccgag cagtcagtga
     2641 acgccagcaa ctgcccgtag cggctccacg tcatctgccg ggtgctgccc gtcgcatccg
     2701 ttgtcgtcgc cggtaactca ctgtgcgcgt catcgtagcg gtagcgtact gtctccccgc
     2761 tgcgcgatgt ctccgatacc agcctgcccg gttcatcata ttcccggcgg ctctccagcc
     2821 cgtccgggga caccaccgcc gtcagctggt tcccgtcgtt atagtaaaat ttcgtctccc
     2881 gcccgtccgg cgtggtgatg tccgtgatat cgcctgacac cacattcaga ccgtactctg
     2941 tccgccgtcc cgccgcatcc gtctgcgccg tgagccttcc tgccgcgtca tacccgctgt
     3001 gcgtgacgct gccgtccgcc agttcttttt tcaccacccg cttcagcccg gccccgcctt
     3061 ctgtatgcag cacctcacgc cggttcaggc tgtccgtgac ggtgatgcga tcctgctcat
     3121 actggtagcg gtaactcagg cctgccgggt taagttgctc caccacccgc cccgcatcgt
     3181 cgtagcggta gcgcatctcc ggcctgcctg cataacggtg cgccaccatc cggcccgggt
     3241 gctgcgcgtc atacgtgaaa gcgcgcacct gcgtattgct gcggtcatat accgccagca
     3301 gttcaccggc ttccgtatat gtgtaccgca ccagtggcgc agcgggcagg ctctccgggt
     3361 atgccgggtc gtgcatcagc cacaccgccg aaaggcggat acccctgtcg gggccgtatt
     3421 cggtaccggg cagtgtgtcg gggaacgctg aggctgagag agggcgggaa ctgtcagaag
     3481 aagatagcga agaggtgcgg gcctcttccg cacgctgcgc ctgcgtggtc agcaccagac
     3541 ggaactcccg cccggcaccg tccgtcacgc cggtgatttc cccggtcagg tcaccggcgg
     3601 cctcacgccg gtacgtcagc gtccgcccga agcggtccgc cagccccgtc agctcccggt
     3661 acggcggcag cggcgctggc agcacgtcct cagcacccgg cacccgctct gaccagccga
     3721 gtatccacca cggcccctgt gcgctgttgg tcgccaggta aagatgcggg cttaaccgga
     3781 tatccggcgg cagcgccccc cacagccgcg ccagcgtatg gccgtccggc tgtgctgcct
     3841 taccaccgcg caccagccac attgactcac tgcggctgta caccgcctcc cccggcagca
     3901 gcggctcaaa gtgaatgctc cgcccgccgt tgtcgttgag tatcagtccg tcatcacgta
     3961 gctgtaagcg gatatcagaa ggcgctttcc agccggggcc gaaaacgccc accggtgcag
     4021 gcgtcttcgt ccggtagctg ctgtaggtgc gggagagaat gaacggcagc gggccgggca
     4081 gcgcaaggtc cgtctcgccg ggcagcacct tcgccccaag cagcggattt accgggttgc
     4141 ccgaagtcat cccgcccgga cacaccgagc acgccacgcc ggtgggcgcg ccaattctta
     4201 cacctgccga accctggaca atgggaccgc catactgagt catatctccc tgacgcgccg
     4261 ctggttttcc gctcatgatt caaattcctt cttctgttta tttttaatgc tggtttgtta
     4321 ctgaattccg aacaggtacg ggatcagtac tctctcatat ccggcgtatc ctgcttttca
     4381 tcaggggtct tcggtgggga tttcgaggct gcacagttcc agttaatcaa cagtgagtca
     4441 ccattaacct gctccttacc ctgcaaatga atttttgtgc cgttgagaaa aatttgtccg
     4501 tccttcgtca gcaccagcct tgccttacca cagcagagct ccagatgctc accggcggag
     4561 taaatcgcgg tctgcccggt atcatccttt ttcgttttac caacggtgaa ggtaacgtta
     4621 tttccgactt tgacgtcata acccagcccg accctcaacg atttatgcaa accgatctgc
     4681 gaggactgca tcagtgccac cgaggtgttc ataatgccac ctaccgtcgt ctgatacgcc
     4741 acgcccacgg tcagcgcacg aacaagtcca atcgtttcca cctgaacaga acccacttta
     4801 attatctggt tactgcccac cgtctctatc tggttaacac ccaccgtctg tatctgattg
     4861 ttggtaaccg caatcatctg attgttgcca atggtttcag catggttgtt tatcacatca
     4921 gtggtgcggt tattcagcac ctcggtgttc atgttcttct gcgcgtggat gtagacctgt
     4981 tctttccctg tcgcatcgtc aaacttcagt tcattaaacc cgctgccctt ataggttttc
     5041 gaacgaatgg tcatctgcgt cttcgtcccc ggcaggctgc cgggtgtgcg gttttcctgg
     5101 tggtaggtac gccccataat aatcggctgg tccgggtcgc cgttgaggaa gtccacaatc
     5161 acctcctgac ccactcgcgg tatcgccagg ttgccaaagc cggtgcctgc ccacgcctgt
     5221 gccacacgga tccagcatga actgtcctgg tttgacgggt tatagcggtc ccagttaaat
     5281 ttcacccgca cgcggccatg ttcatcacag aagatttcct cgcctgccgg tcccgtcacg
     5341 acggcgctct gcgggccgtc caccagcggt ttcagcaacg gctgtggccg ccatgtccgg
     5401 tctgccggta ttaccgcaaa gtggttattc agcgtggtac ctgaaccact gcgtcccggc
     5461 accgcctgcg gctgctcgcc gtgcagttca cttgccacca cctgccattc ccggttcagg
     5521 ttcgcctgcg gatgccccgt cagcgcaatt cgccgtcccg gccatatctc cggagaacgg
     5581 cttgttccgc gcgccacttc tgcgttgttt cgccagccat ccatctgcca gcgggcaaag
     5641 ttctgcccgt gggcaccctt gaaacgtccg gggtagtcat acacttcata ctgtgtgcgc
     5701 tggtaatcct ggtactggcc ttcctgatca aaacgccctg cccagccggg tcgtttaaag
     5761 gtgtagtcct tggtcaccac ggaagaaggg cggatttgtg cgctgtagcg gaactggctg
     5821 atgcagaggg tgctcacctc ggtacgggtg ttcggattcc aggggatctc aaaggactcc
     5881 ggcagataac gcacggtgtc gcacaggacc aggctctggt cggtactttt ttgcgcgtgc
     5941 tcctcataaa agaagatgcc ttcctccgcc gccatccggc acaggaaatc gtaatcggtt
     6001 tcgccgtact ggacacaaaa ctcacgggaa ggatgcggct cgctgaacag cggactccac
     6061 tcggtcacac cgttttcctt caggatcgta gcgaggatgc tttcgatgtc ctcattctgg
     6121 aagatacgga agttctggcg cagccctgtg cgccacagcg gcgggcacac cttcatgctg
     6181 tacagcatct ggtttttgtc gttctccccc agttcaaacc aggtcaccac acctttcacc
     6241 cggcgctgta cgtcatcgcc ctgccatacc gtcaggtagg ccattttgtc cagcacctgc
     6301 gcaaattcaa gggaaagaaa ctgctgactg accagagaaa ggtcgaggga aaaaagtgaa
     6361 gagagtgact gggtgagatg aaaggaaacc accgcaaacg cgtccggtgg caggccgtcc
     6421 acttcaagtg tgaaacgtaa tccggttgac atagcaacct ccgtgaaaat gaataataat
     6481 ccgcacatat tgcatgtaca acatgtcggg cggatgagat aagcgagtcg acatctggcg
     6541 ttaccgggga atatcagaaa aatgtcatgg ttatgtgatg atttagtctg ggattttact
     6601 tcagagaaac gggtgtctgt ttagtcaaca aaataatcac aaacgcaacg gatgtgttct
     6661 cgacgttatt cacgacaggc tcgtacatta cagcgagtaa taaaaaacct gatatgcaaa
     6721 gtcaaaccaa tattcataat gtctttttat tcacaacaat aaataattca ataacaatca
     6781 ttgaaatgaa tgatgatttg tataaggaat atacgctacc gccccaccct gataacatga
     6841 tctgcacatt gccatcttaa cgtcgttaaa cactggctta atagtggata tttcaaatta
     6901 ggaatttatt aaattcacct gatgaatatc ataagcaaat agcatagaaa ataatgacaa
     6961 ttcccccgag gggaccattt ccaaagcact gcaccaccac caggtgcccg gatgatcttg
     7021 cttcagtggt aagagtacct gattcattgt tcccaaaatg atgttttccc c
//
LOCUS       DAFNBJ010000214         7061 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000214 DAFNBJ010000000
VERSION     DAFNBJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.214"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            469..1458
                     /gene="araF"
                     /locus_tag="L2X49_004453"
     CDS             469..1458
                     /gene="araF"
                     /locus_tag="L2X49_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HBN4799434.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1528..3042
                     /gene="araG"
                     /locus_tag="L2X49_004454"
     CDS             1528..3042
                     /gene="araG"
                     /locus_tag="L2X49_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBN4799435.1"
                     /translation="MQQSTPYLSFRGIGKTFPAVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3057..4043
                     /gene="araH"
                     /locus_tag="L2X49_004455"
     CDS             3057..4043
                     /gene="araH"
                     /locus_tag="L2X49_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBN4799436.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4210..5010
                     /gene="otsB"
                     /locus_tag="L2X49_004456"
     CDS             4210..5010
                     /gene="otsB"
                     /locus_tag="L2X49_004456"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBN4799437.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGPGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4985..6409
                     /gene="otsA"
                     /locus_tag="L2X49_004457"
     CDS             4985..6409
                     /gene="otsA"
                     /locus_tag="L2X49_004457"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HBN4799438.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6416..6844)
                     /gene="uspC"
                     /locus_tag="L2X49_004458"
     CDS             complement(6416..6844)
                     /gene="uspC"
                     /locus_tag="L2X49_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBN4799439.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1794 a         1644 c         1827 g         1796 t
ORIGIN      
        1 atggctctca ttatacgtgt tctgttaatt aatgagcaca gtgataatta atggcgaaat
       61 gaatgacgtg cattttccac atctttgagt tgcggttatt acaccatttc aaaaactcaa
      121 cgccaggtaa tgcggcctat tgactggtta aaaagaagac atcccgcatg ggtaccaaag
      181 acaacaagga tttccaggct aatcttatgg attaatctgc tgtgcattcg acaatttgtc
      241 tgacaaattg gctttccctt atgtcttttc ccgctaaatt tatgcacgtt ctcactgtaa
      301 ttctgcgatg tgatattgct ctcctatgga gaattaattt ctcgctaaaa ctatgtcaac
      361 acagtcactt atcttttagt taaaaggtaa tgctttgttt tccgattaat ttaacgaatg
      421 tcattcgttt ttgccctaca caaaacgaca ctaaagctgg agagaaccat gcacaaattt
      481 actaaagccc tggcagccat tggtctggca gccgttatgt cacaatccgc tatggcggag
      541 aacctgaagc tcggtttcct ggtgaagcaa ccggaagagc cgtggttcca gaccgaatgg
      601 aagtttgccg ataaagccgg gaaggattta gggtttgagg ttattaagat tgccgtgccg
      661 gatggcgaaa aaacattgaa cgcgatcgac agcctggctg ccagtggcgc aaaaggtttc
      721 gttatttgta ctccggaccc caaactcggc tctgccatcg tcgcgaaagc gcgtggctac
      781 gatatgaaag tcattgccgt ggatgaccag tttgttaacg ccaaaggtaa gccaatggat
      841 accgttccgc tggtgatgat ggcggcgact aaaattggcg aacgtcaggg tcaggaactg
      901 tataaagaga tgcagaaacg tggctgggat gtcaaagaaa gcgcggtgat ggcgattacc
      961 gccaacgaac tggatacggc acgtcgccgt acaacgggtt ctatggatgc gctgaaagcg
     1021 gccggattcc cggaaaaaca aatttatcag gtacctacca aatctaacga catcccgggg
     1081 gcatttgacg ctgccaactc aatgctggtt caacatccgg aagttaaaca ttggctgatc
     1141 gtcggtatga acgacagcac cgtgctgggc ggcgtacgcg cgacggaagg tcagggcttt
     1201 aaagctgccg atatcatcgg cattggcatt aacggtgtgg atgcggtgag cgaactgtct
     1261 aaagcacagg caaccggctt ctacggttcc ctgctgccaa gcccggacgt acatggctat
     1321 aaatccagcg aaatgcttta caactgggta gcaaaagacg ttgaaccgcc aaaatttacc
     1381 gaagttaccg acgtggtgct gatcacgcgt gacaacttta aagaagaact ggagaaaaaa
     1441 ggtttaggcg gtaagtaatt tgccggaaaa attcccctct gcatgatgca gagggggtgt
     1501 gaacgaccag tgattcacgg agacgttatg caacagtcta ccccgtatct ctcatttcgc
     1561 ggcatcggta aaacgtttcc cgccgttaag gcgctgacgg atattagttt tgactgctat
     1621 gccggtcagg ttcatgcgtt gatgggtgaa aatggcgcag gaaaatcaac tctcttaaaa
     1681 atcctcagcg gcaactatgc gccaaccacg ggttctgtag tgattaatgg gcaggaaatg
     1741 tccttttccg acacgaccgc agcacttaat gcgggtgtgg cgattattta ccaggaactg
     1801 catctcgtgc cggaaatgac cgtcgcggaa aacatctatc tcggccagct gccgcataaa
     1861 ggcggcattg tgaatcgctc attgctgaat tatgaggcgg gtttacaact taaacatctt
     1921 ggtatggata ttgacccgga cacgccgctg aaatatctct ccattggtca gtggcagatg
     1981 gttgaaatcg ccaaggcgct ggcgcgtaac gctaaaatta tcgcctttga tgagccaacc
     2041 agctcccttt ctgcccgcga aatcgacaat cttttccgcg ttattcgtga actgcgaaaa
     2101 gaggggcggg tgatcttata cgtttctcac cgtatggaag aaatatttgc cctcagcgat
     2161 gccatcactg tctttaaaga tggacgttat gtcaaaacct ttaccgatat gcagcaggtt
     2221 gaccacgacg cgctggtgca ggcgatggtc gggcgcgaca ttggcgatat ctacggctgg
     2281 caacctcgta gttatggcga ggagcgcctg cgtcttgatg ctgtgaaagc accaggcgtg
     2341 cgtacgccaa taagtctggc ggttcgcagt ggtgaaattg ttgggctgtt tggtctggta
     2401 ggagcggggc gtagcgaatt aatgaaaggc ttgtttggcg ggacgcaaat caccgccggt
     2461 caggtttata tcgaccaaca gccgatcgat attcgtaaac cgagccacgc cattgccgca
     2521 ggcatgatgc tctgcccgga agatcgcaaa gcggaaggca ttattcccgt gcactccgtt
     2581 cgcgacaata tcaacatcag tgccagacgt aaacatgtgc tcggcggttg tgtaatcaac
     2641 aacggttggg aagaaaacaa tgccgatcac cacattcgtt cgctcaacat caaaacgccg
     2701 ggcgctgagc aactgatcat gaatctctca ggcggaaatc agcaaaaagc cattctgggc
     2761 cgctggttat cggaagagat gaaggtcatt ttgctggatg aacctacgcg cggcattgat
     2821 gttggtgcta agcatgaaat ttacaacgtg atttatgcgc tggcggcgca gggtgtggcg
     2881 gtgctgtttg cctccagcga cttacctgaa gtcctcggcg ttgccgaccg gattgtggtg
     2941 atgcgggaag gtgaaatcgc cggtgaattg ttacacgagc aggcagatga gcgtcaggca
     3001 ctgagccttg cgatgcctaa agtcagccag gcagttgcct gagtaaggag agtatgatgt
     3061 cttctgtttc tacatcgggg tctggcgcac ctaagtcgtc attcagcttc gggcgtatct
     3121 gggatcagta cggcatgctg gtggtgtttg cggtgctctt tatcgcctgt gccatttttg
     3181 tcccaaattt tgccaccttc attaatatga aagggttggg cctggcaatt tccatgtcgg
     3241 ggatggtggc gtgtggcatg ttgttctgcc ttgcttccgg tgactttgac ctttctgtcg
     3301 cctccgtaat tgcctgtgcg ggtgtcacca cggcggtggt tatcaacctg actgaaagcc
     3361 tgtggattgg cgtggcagcg gggttgctgc ttggcattct ctgtggcctg gtcaatggct
     3421 ttgttatcgc caaactgaaa ataaatgctc tgatcacaac actggcaacg atgcagattg
     3481 ttcgaggtct ggcgtacatc atttcagacg gtaaagcggt cggtatcgaa gatgaaagct
     3541 tctttgccct tggttacgct aactggttcg gtctgcctgc gccaatctgg ctcaccgtcg
     3601 cgtgtctgat tatctttggt ttgttgctga ataaaaccac ctttggtcgt aacaccctgg
     3661 cgattggcgg gaacgaagag gctgcgcgtc tggcgggtgt accggttgtt cgcaccaaaa
     3721 ttattatctt tgttctctct ggcctggtat ctgcgatagc cggaattatt ctggcttcac
     3781 gtatgactag tgggcagcca atgacgtcga ttggttatga gcttattgtt atctccgcct
     3841 gcgttttagg tggcgtttct ctgaaaggtg gcatcggaaa aatctcatat gtggtggcgg
     3901 gtatcttaat tttaggcacc gtggaaaacg ccatgaacct gcttaatatt tctcctttcg
     3961 cgcagtacgt ggttcgcggc ttaatcctgc tggcagcggt gatcttcgac cgttacaagc
     4021 aaaaagcgaa acgcactgtc tgatgctttt ttccacaaca atttaacgtt ttttcccacc
     4081 acagccagcc gccacaacgg ttggctgttc ttcattgcaa atggcgaccc ccgtcacact
     4141 gtctatactt acatgtctgt aaagcgcgtt ctgcgcaaaa caataagaaa agagaaggag
     4201 gagagccggg tgacagaacc gttaaccgaa acccctgaac tatccgcgaa atatgcctgg
     4261 ttttttgatc ttgatggaac gctggcggaa atcaaaccgc atcccgatca ggtcgtcgtg
     4321 cctgacaaca ttctgcaagg actacagcta ctggcaaccg caagtgatgg tgcattggca
     4381 ttgatatcag ggcgctcaat ggtggagctt gacgcactgg caaaacctta tcgcttcccg
     4441 ttagcgggcg tgcatggggc ggagcgccgt gacatcaatg gtaaaacgca tatcgttcat
     4501 ctgccggatg cgattgcgcg tgatattagc gtgcaactgc atacagtcat cgctcagtat
     4561 cccggcgcgg agctggaggc gaaagggatg gcttttgcgc tgcattatcg tcaggctccg
     4621 cagcatgaag acgcattaat gacattagcg caacgtatta ctcagatctg gccacaaatg
     4681 gcgttacagc agggaaagtg tgttgtcgag atcaaaccga gaggtaccag taaaggtgag
     4741 gcaattgcag cttttatgca ggaagctccc tttatcgggc gaacgcccgt atttctgggc
     4801 gatgatttaa ccgatgaatc tggcttcgca gtcgttaacc gactgggcgg aatgtcagtg
     4861 aaaattggcc caggtgcaac tcaggcatca tggcgactgg cgggtgtgcc ggatgtctgg
     4921 agctggcttg aaatgataac caccgcatta caacaaaaaa gagaaaataa caggagtgat
     4981 gactatgagt cgtttagtcg tagtatctaa ccggattgca ccaccagacg agcacgccgc
     5041 cagtgccggt ggccttgccg ttggcatact gggggcactg aaagccgcag gcggactgtg
     5101 gtttggctgg agtggtgaaa cagggaatga ggatcagccg ctaaaaaagg tgaaaaaagg
     5161 taacattacg tgggcctctt ttaacctcag cgaacaggac cttgacgaat actacaacca
     5221 attctccaat gccgttctct ggcccgcttt tcattatcgg ctcgatctgg tgcaatttca
     5281 gcgtcctgcc tgggacggct atctacgcgt aaatgcgttg ctggcagata aattactgcc
     5341 gctgttgcaa gacgatgaca ttatctggat ccacgattat cacctgttgc catttgcgca
     5401 tgaattacgc aaacggggag tgaataatcg cattggtttc tttctgcata ttcctttccc
     5461 gacaccggaa atcttcaacg cgctgccgac atatgacacc ttgcttgaac agctttgtga
     5521 ttatgatttg ctgggtttcc agacagaaaa cgatcgtctg gcgttcctgg attgtctttc
     5581 taacctgacc cgcgtcacga cacgtagcgc aaaaagccat acagcctggg gcaaagcatt
     5641 tcgaacagaa gtctacccga tcggcattga accgaaagaa atagccaaac aggctgccgg
     5701 gccactgccg ccaaaactgg cgcaacttaa agcggaactg aaaaacgtac aaaatatctt
     5761 ttctgtcgaa cggctggatt attccaaagg tttgccagag cgttttctcg cctatgaagc
     5821 gttgctggaa aaatatccgc agcatcatgg taaaattcgt tatacccaga ttgcaccaac
     5881 gtcgcgtggt gatgtgcaag cctatcagga tattcgtcat cagcttgaaa atgaagctgg
     5941 acgaattaat ggtaaatacg ggcaattagg ctggacgccg ctttattatt tgaatcagca
     6001 ttttgaccgt aaattactga tgaaaatatt ccgctactct gacgtgggct tagtgacgcc
     6061 actgcgtgac gggatgaacc tggtagcaaa agagtatgtt gctgctcagg acccagccaa
     6121 cccgggcgtt cttgttcttt cgcaatttgc gggagcggca aacgagttaa cgtcggcgtt
     6181 aattgttaac ccctacgatc gtgacgaagt tgcagctgcg ctggatcgtg cattgactat
     6241 gtcgctggcg gaacgtattt cccgtcatgc agaaatgctg gacgttatcg tgaaaaacga
     6301 tattaaccac tggcaggagt gcttcattag cgacctaaag cagatagttc cgcgaagcgc
     6361 ggaaagccag cagcgcgata aagttgctac ctttccaaag cttgcgtagg agcggttaat
     6421 cacccgtaag tggaactaac agcacatcga cctcacttga ggcaatcact cttttcgccg
     6481 agcaggatgc tcgcgaaaag aaactgtgat tgtgattacc gcaaatcacc aaatcgaaat
     6541 gatgtttgtg acatacttcc agaatatgtt cgcttaactc accgtaggca ataaacgtct
     6601 tgtcaacggg ataccccgca tcctgaatta acttatcaag aaagctttgc gtttcttcct
     6661 gcatcacgct gcgtaaatct tcaagcatcg gcgcagctaa ttgattgtac atttccgggt
     6721 cggaagcgag agtaattaaa ctgatgtgtc cctttactgg cctggcgata gataccgctt
     6781 ttgccagcag ttgctgactt tccggtgtaa ccgcaacagc gacaagaata ttgctatagc
     6841 tcataacctg ttccttattc tgtgaacttc aggtgacatt aaagctttac tcctaatgga
     6901 gaaacgacgc aatcccaact cggtcaaaca ggtaaatatt agtctgatta ttagtaaaga
     6961 caattaatca atcactcccg gtgatcatat aatttcaaat gtaattataa gaaaatagtg
     7021 tactgattct ccgcattaaa caagtggttg atatatgtaa a
//
LOCUS       DAFNBJ010000215         6923 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000215 DAFNBJ010000000
VERSION     DAFNBJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.215"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            82..1428
                     /locus_tag="L2X49_004459"
     CDS             82..1428
                     /locus_tag="L2X49_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799440.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMVLHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            1707..2606
                     /locus_tag="L2X49_004460"
                     /pseudo
     CDS             1707..2606
                     /locus_tag="L2X49_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2794..3981)
                     /locus_tag="L2X49_004461"
     CDS             complement(2794..3981)
                     /locus_tag="L2X49_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBN4799441.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKACAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICRQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMRMWRYRITRLLSRKSPDLVIPDELAAEGIGRR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRFLEEIVYVITE
                     TVRKTAKQIRWRGMYQRLLKVDPLKCILCGSQMWFTGLKRGYRLAELVGMHEPLARQR
                     VCG"
     gene            complement(3988..4353)
                     /locus_tag="L2X49_004462"
     CDS             complement(3988..4353)
                     /locus_tag="L2X49_004462"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799442.1"
                     /translation="MARSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGITPPF"
     gene            4443..5423
                     /locus_tag="L2X49_004463"
                     /pseudo
     CDS             4443..5423
                     /locus_tag="L2X49_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
     gene            complement(5441..5788)
                     /locus_tag="L2X49_004464"
     CDS             complement(5441..5788)
                     /locus_tag="L2X49_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HBN4799443.1"
                     /translation="MKLDISVKYLLKSLIPSLIILTVFYLGWKDNQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFIFCKALSGK"
     gene            5907..6254
                     /locus_tag="L2X49_004465"
     CDS             5907..6254
                     /locus_tag="L2X49_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799444.1"
                     /translation="MTFEQKKARAIALMDSKNMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLMASFHWW
                     RRKVNRLPSWDDV"
     gene            complement(6272..6862)
                     /locus_tag="L2X49_004466"
     CDS             complement(6272..6862)
                     /locus_tag="L2X49_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799445.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTQDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGMTYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRLEGDPLEVAQWLQACHDAGMEVRVQVNESVTP"
BASE COUNT         1817 a         1686 c         1692 g         1728 t
ORIGIN      
        1 tcatctctgc ctttgtttca tggggtgttc tttgtatatt ttacttactg tttaagcatg
       61 tacataagga gtgctttcat tatgccaatg atacttgtaa agaagaatcc ccggggcaaa
      121 gtgattcggg agcttagcag tgaggaagag actgcagtaa agacggtctg tggactgaaa
      181 aagccagcaa ccatggtact acataacctg gccaatgata ttctccggga aatgcgtgag
      241 tatgatgcat ggttacagtg tgactgtatt ccgggagatt cgccggccat gaattttgct
      301 gctcttaaaa acaatacggg tactctttat ctgtccagtt ttaatcatga gcatgcacct
      361 gaatgcccca tgtatcgtca gttatccgga aatgaagagg aaacatcttc cggagcaagc
      421 cggcatcccg ttagcacccg tataaattac agaaactttt taccacctga tgacagtaat
      481 tctgttatca gactgcaggc ccgctctgct taccataatg gtgagagctc ctccgttcgc
      541 aagaaacgtc cgcgccttgc ccgtttactc ctgtctctta ttgacgatgc tgggctgaat
      601 aaactggatt ccctggctaa tccccgtatc cggactaatt atcgtgagtg tcttgatgcc
      661 atacggcagg ttacactgca gcaggagtat attcggggaa gatccttatc tgaaattatc
      721 catttccggc ctggaatgtc tgaaaggtct caggagcggt taatggaaac gctggagaat
      781 tcagaacgac actggcctgc cagaagaaag catatgttct ttcagatttt tatggcgcag
      841 cacatctgtc gtgatgctgt ggaaattcac tgggcgaacg gtaatattca ggtcatcaga
      901 cctgtgcggg ggatcagtat taatggtgaa gcgcagggcg gaatacgtcc cccttactgg
      961 gttattcttg ctttttgccg gagtgccgat ggcagaatca tctgcagtga agggtatgct
     1021 catgctcttt atcaactgac atgtccggtg cctgtggaca gcaaactgga acgaaacacg
     1081 ctcactgctc ttctgaatgt ggccagctgg cttaaaagaa agccaggtac gccggaatta
     1141 agtctggaaa ggcccctgtt tgatacagaa gtttatgtta atggtgaaaa gaaatatgtc
     1201 ctgccggatt tcattgtcac agcaagggct cctgacggaa agacggccag agtggtcatc
     1261 gaaacgatgg gatatgaaga cagtgattac tgcgcgagaa aatccaggca gcataccggc
     1321 atgaagcaga ttggtgttct gcataccgat ccaccgaaat ggctggataa cgatcatccc
     1381 ccttttgaga aacatatgta cggtgttttt atgcatctca ggtactgaga tattttgtgg
     1441 ctcagttctg taacttttcc cgtaacattg tctgttgtta cgggaaagtc cgttttttgt
     1501 attgcaccag agaataccca gactgtgatg ctgccacagc gtcagcaggc tttctgaacg
     1561 gtgtaacatc acttcatgtt aatgataatc actatcatta aatcttgaca tgccattttc
     1621 tccttaataa attaatactg tatatgtatc catatacgta agcagttaat tcatttgttt
     1681 tcctcagagg atgaaggaga taccgaatgt ctgaccctgt acgtattaca aatcccggtg
     1741 cagaatcgct ggggtatgat tcagatggcc atgaaattat ggccgttgat atttatgtaa
     1801 accctccacg tgtcgatgtc tttcatggta ccccgcctgc atggagttcc ttcgggaaca
     1861 aaaccatctg gggcggaaac gagtgggtcg atgattcccc aacccgaagt gatatcgaaa
     1921 aaagggacaa ggaaatcaca gcgtacaaaa acacgctcag cgcgcagcag aaagagaatg
     1981 agaataagcg tactgaagcc ggaaaacgcc tctctgcggc gattgctgca agggaaaaag
     2041 atgaaaacac actgaaaaca ctccgtgccg gaaacgcaga tgccgctgat attacacgac
     2101 aggagttcag actcctgcag gcagagctga gagaatacgg attccgtact gaaatcgccg
     2161 gatatgatgc cctccggctg catacagaga gccggatgct ctttgctgat gctgattctc
     2221 ttcgtatatc tccccgggag gccaggtcgt taatcgaaca ggctgaaaaa cggcagaagg
     2281 atgcgcagaa cgcagacaag aaggccgctg atatgcttgc tgaatacgag cgcagaaaag
     2341 gtattctgga cacgcggttg tcagagctgg aaaaaaatgg cggggcagcc cttgccgttc
     2401 ttgatgcaca acaggcccgt ctgctcgggc agcagacacg gaatgaaagg gccatttcag
     2461 aggcccggaa taaactcagt tcggtgacgg aatcgcttaa cacggcccgt aatgcattaa
     2521 ccagagctga acaacagctg acgcaacaga aaaacacgcc tgacggcaaa acgatagttt
     2581 cccctgaaaa attcccgggg cgttcatcga taggaattaa aatcccaaaa agattaaaaa
     2641 aacaccacaa aacggatgtt gcttcaacac tccttgtgca ccatatgaac agaaatgact
     2701 attaaacagg atgactctga ttgatacagg gtatgattgg cggttttttg ctccatcccc
     2761 cgtaaaatgg acgcaacttc ccctctgcgg ctctcagccg cacacccgct gtcgcgccag
     2821 tggctcatgc atcccgacca gctctgccag acggtagccc ctcttcagtc ccgtaaacca
     2881 catctgactt ccgcaaagaa tgcatttcag cgggtcaacc ttcagtaacc gctgatacat
     2941 ccctctccac ctgatttgtt tcgccgtttt tctcaccgtc tccgttatga cgtacacaat
     3001 ttcttccagg aagcggcgct tcaccggact caggaaaccg tagtacctca ccatacggaa
     3061 acccttatcc gccacatgcc aggagaacct ttccataaac tcatcgccac tcatcaccag
     3121 gtattcttcc cgttttgtcc ggtggctgtt ataacgcaga ccaatttcat cctgaccggc
     3181 ataatgctcc agacggctca ttggcaccgg tggctttttc aggtaagaac caaagtacac
     3241 cgccacatgg gtggcgttat ccatcaccct ggatacgttg acattccagc cacggcggta
     3301 atgtgtgtcc aggaagcgat tccattcccg tctcccgatt ccttctgctg ccagctcatc
     3361 cggtatcacc aggtcagggg atttccgtga cagtaaccgc gttatccggt aacgccacat
     3421 cctcatcacc ttacgggcgt aaaaatgaag gtttttccag gtgtggcctg acgtcactcc
     3481 gccggctgtt gttgacaggt gaatgtgcgg atgccactgc tggtctcgtc cccatgtgtg
     3541 gattaccgtg aatatccccg gctccacatc tgcctggcgg cagatttcca gtatcacatc
     3601 cgcagcaatg cggctcatct ctgccagcaa ccagcggttg tgaaacacca gggaccagta
     3661 ttggcacgga agtgtgaaca caatatgctg ccacgggcag tcgggaacca gactcagcag
     3721 atactgtatc cactgtgcgc aggccttcac tccgcagtgc ggacaggagc ggcttttaca
     3781 ccggaagcag acctttttag tgtggcaaca gtccggagat gaacaacacc actgcgtgta
     3841 ccccatcagg gtagtcccgc acgccatgat ttttgtcacc gattcaatga ctaccggacg
     3901 cactgaacct tccggctgtt tctccagcca gttaagccag cggtttccct gctggaagat
     3961 atcggcaaaa cggggaagca tctgatatca gaagggcggg gtaatgccat ccggccagtg
     4021 aactgtgcca cactccgggc agtacatgcc gccggcgctg ataccggcaa gaatggtcgc
     4081 aaactcccgc tccgtgcagc gggctatttc aggataccct tcgtcatcaa cacgtacaaa
     4141 ccagaagacc agctttttgt ttccggcatc cacaaagaag ggaatattca ggtctgcgca
     4201 gcactcaacg gcatcgtcaa aattatcaaa gcgcagagct tctgcgtctt cttcgtcaaa
     4261 gaagtcatcg tcgtgaagct tcacgacata gcggggaagt ttgcttcttt gaggggcggg
     4321 ttttcgttta cggggtttag ctgagcgggc catataacta cctgaaagac aatgatattg
     4381 tctgttttta taacggtaat ttcaggccat tacaagccgc agccgtcagg ctgcctactc
     4441 gatcaacaaa tcattctatt gttgtgagtg gtgatccgag atttgccggt acgataaaaa
     4501 tcacaaccag cgcggtcatc gataaccgtg caaacctgaa ttatcttctg acccattccg
     4561 gtctggacta taaacgcaat attctgaatg accggaatcc ggtggtgaca gaggatgtgg
     4621 aaggtgacaa gaaaatttat aatgctgaag ttgctgaatg ggataagtta cggcaacgat
     4681 tgcttgatgc cagaaataaa atcacctctg ctgaatctgc ggtaaattcg gcgagaaata
     4741 acgtcagtgc cagaacaaat gaacaaaagc atgcaaatga cgctcttaat gccctgttga
     4801 aggaaaaaga gaatatccgt agccagattg ctgacatcaa tcataaaata gctgaagaga
     4861 aaagaaaaag ggatgaaata aatatggtaa aggatgccat aaaactcacc tctgatttct
     4921 acagaacgat atataatgag ttcggtaaac aagcatccga acttgctaag gagctggctt
     4981 ctgtatctca agggaaacag attaagagtg tggatgatgc actgaacgct tttgataaat
     5041 ttcgtaataa tctgaacaag aaatatagca tacaagatcg catggctatt tctaaagccc
     5101 tggaagctat taatcaggtc catatggcgg agaattttaa gctgttcagt aaggcatttg
     5161 gttttaccgg aaaagttatt gatcgttatg atgttgctgt ggagttacaa aaggctgtaa
     5221 aaacagacaa ctggcgtcca ttttttgtaa aacttgaatc actggcagca ggaagagctg
     5281 cttcagcagt tacagcatgg acgttttccg tcatgctggg aacccctgta ggtattctgg
     5341 gttttgcaat tattatggct gcagtgagtg cgcttgttaa tgataagttt attgagcagg
     5401 tcaataaact tattggtatc tgacacataa tgatgccctt tcattttcct gaaagggctt
     5461 tgcagaatat aaatattaag taaatggcaa ccacaggaac tgatataacg aaacagaata
     5521 attcaaatat tgcagttaag cttccgccaa ctggatttgt aaagaagtct ttttgccaga
     5581 attctttccc tgtaaacctt attaccattt tctgtattat cctcattgaa aaaggaaaag
     5641 taatggcgct gataatgcat ccgatgaacg cataaaacat tcttgcattt tcctggttat
     5701 ctttccatcc cagataaaaa actgtaagaa taatgaggct tggtatcagg ctcttcagta
     5761 aatactttac agatatatcc agtttcatct ggtttctcct ttattgttat cgtgtaccgg
     5821 tgcgtgataa cataatcaca gtatgataat aataatcaat aacaataagc tgtgtcacgt
     5881 ttacatcatg gaacggaggt gctcctgtga cctttgagca gaaaaaagcc cgggccattg
     5941 ccctgatgga cagcaaaaat atgtggcgga gcaattatgc ccctcctctt ctgcgtattc
     6001 tctggcggct gggtatccgg cttccgccat taccctttat gccgttctgg caggtcacgc
     6061 tgttgatggg gagcttatgg ggcatttcct ggggatgtgc aatgtggttt atctactggg
     6121 ggccgtcagg aatggtggca ggtgaggcca ttattataag tatcacgggt ggttttttgt
     6181 tcggactgct catggcctct ttccactggt ggcgcaggaa agttaaccgg ctgccatcat
     6241 gggatgacgt gtaaaccgtg ctggtatgtt tttacggggt gacgctttca ttaacctgta
     6301 cacgaacctc cattccggca tcgtgacagg cctgcagcca ctgcgccact tccagcggat
     6361 cgccctccag gcgtaccacc ctgccttctt tattccataa ctgcagacag gtgctgccgt
     6421 cgagacgtac cacaaaatcc ccacggcagg cctgatacgt catgccgttc agctccaccg
     6481 ccagatcagt ggcagaagtg ccgtctgcgg gcagacctgc ggccgggacc agcggcagta
     6541 cgacgacttc tgccgcacgc tgttctgccc ggaggcggtc ctgttctgcc agcagcagag
     6601 gctgtgccac ggcccggccg gcgtccgtca gctcgacagc cagctgcaga ttcggggcgc
     6661 gcagggtacg aagccagccg gcatcctcca gacgacggca ggaggcgcgc aggtttggcc
     6721 cgtagaccgg ggcctccccg cttcgttcca gcacccgttc caggtcctgc gtcagcacgg
     6781 gtcccggccg tttttcatca agggcagcca gcacaatcag cactctccgc tgcagtggcg
     6841 acggtcggcg gctctgggtc attacctgtc atcctccggc cagatgaggt ttctgaggcg
     6901 ctgaaagtcg gcgtcaatga gct
//
LOCUS       DAFNBJ010000216         6914 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000216 DAFNBJ010000000
VERSION     DAFNBJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.216"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..2746)
                     /locus_tag="L2X49_004467"
     CDS             complement(<1..2746)
                     /locus_tag="L2X49_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBN4799446.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     H"
     gene            complement(2803..5175)
                     /locus_tag="L2X49_004468"
     CDS             complement(2803..5175)
                     /locus_tag="L2X49_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBN4799447.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTDSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGRQGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNSDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5213..6898)
                     /locus_tag="L2X49_004469"
     CDS             complement(5213..6898)
                     /locus_tag="L2X49_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HBN4799448.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1785 a         1590 c         1558 g         1981 t
ORIGIN      
        1 gatgagaaag atattgttta acggcagtgt taacattctc taccgtcatt tgtttcaaca
       61 attgctcctg ctcagtccat gctgcaggat cgtcatattg aataagacta tttacaatag
      121 tgttcgctaa ttgttgaacg ctacgctgtt ggatatcgag gctgcgctga acgttttgct
      181 ggtattcatt cagttcttgc tcactgatcc ctttagccag acgcttaacc atcacttcat
      241 tcgctaacgt taacagttca tcatgtcgtt ctggttgaca agtaaaagcc agcaaatgac
      301 tgatatcttt ggcctgagga tcaaccgaga ggcgagaaga aacgctgtat gctccagatg
      361 cctgttcacg aatattaaca cgtagatctt ttgccagtgc gacgttaaaa gcatcgagcg
      421 ccatacgcgt cgccagatta acaggtgtcc gggaatcata acgcttccac tgtgaaacct
      481 gtgccacagg ttcattttgt tcttttacag taaccgatgc gttgtccgtc gcgcgagtta
      541 atggtttacc tgcggctaat ggcgaatcag agtgtttgat tgatcctaag taacgcgtaa
      601 ttaacgccac gagtttgtct tctgagacat taccgacaat gacaaacgtg atatccgctg
      661 gagatgaaaa caactggcga tcggcagcca gcgcatctgc ggcagtaaac tgtacaatct
      721 gattttcttg cagtaatttc gtgcggtcat cagcatagcg cgtctcatac atctgctggg
      781 cgaatttctc cgctggacgc tggtcgagcg ttttcaacgc ctgaatttga gcattttgta
      841 gcgatgccca aatattgtcg ttaatcgtgc tgtgggtgat tcgctggtta atcaactgaa
      901 aaccaggttc agggttatta gtccgcgcgc taacggagag caacgtattc atgccactga
      961 ctttactgct catggttacc gaattttccg cactccagcg tttcaggctg gaagaggaga
     1021 gctcaccaac gccgcttccg cttactgctt tatttgccag cgcgataagt gatttttgct
     1081 gcgcagggaa acttaaatcg cctttattcg atacggcagt aatttgtagc ttttgctctt
     1141 cacccgcgga ttttgccaga ataaccctgg caccattgga aagtgttaat gatgtcagat
     1201 tctcagccag cgtttctttg ctgctaattt ccgcctgtgg gtcagcgtct actgttaacg
     1261 ataaatttct gcctgggaag atgtaagccg ccagcttttt gttggcgtac tctttttcca
     1321 gcgccaggat agccgcagga gacaatgctt ttttggcggc aagctcattg tttaccattt
     1381 gctcccaaaa tgcgtcctgg ttctttctta actgctgcca tttttccgcc agactttgca
     1441 cggtaatttg ctgccacaga cgtttcgaaa gttgatatgt ctcttccggc gacaagaacg
     1501 gcgtattatt taatgagctg gatgccaggc gactggtcag catacgtaaa tcacgttcag
     1561 cttgctgatc aaccgcattt ttcagccagg tgaggcgggt agatttgaca tcatcgagtt
     1621 cttcagcaga aaaaccatgc tgatcaatgg ttgccaactc tgccattaat gcattcgcag
     1681 catcctgcat attatcgtct cgtgcattta cacggaaaaa cagcgactga taatcgggtg
     1741 caattttaac gctgcgcgca gtgccgccag aaatagtctt caactcgccc gactgtatgc
     1801 gttcctgcag acgttgattg aacagctgaa ctaacatgct ccattcagct tgttcgacaa
     1861 agctttgctc atcgttcact tgtaccattg gcaggcgata atagagtgcg atgccgttca
     1921 cccggttttc tttatcatta atgatattaa agcgcaggtg gttttcggct tttgtcggcc
     1981 agacgcgatt ttcagctgct ttgttagccg gaagcttact taaattatcc tttatcagcg
     2041 ccagcgcttc tttactgtcg atatcgccga ccacgataaa ggtcatatta tttggttgat
     2101 accagcgttg ataaaattgg cgcaattgtg ccggtgtgac cgtggcgaca gtatccatca
     2161 ggccgatagg ttcacggtct aaattacggg tatttgccag caggaaaggg cggcgcgcct
     2221 gagaggtgcg ccatttcgca tcctgatggg cacgccattc ctcagtaatt acgccacgtt
     2281 cagcgtctac ttcgagtttt tcaaaggttg cggcattact ccattcactg aagattgcca
     2341 tcacttgttg cagattttgt ttctgcgtag tcggcaaact cacctgatac accgtttcgt
     2401 catagctggt ataggcatta acatcgcgac caaaacgcag gcccattgac tcaaatgttt
     2461 cgatgacttt attacccggc catgtttttg tgccgttaaa catcatatgt tctacaaaat
     2521 gagccacgcc gcgctcattg tcttcttcct gcaatgaacc ggtatgaatt tgcagccata
     2581 aatttacctg atcctttgga tgagcatgcg gataaatcat atatcgcaag ccattgtcca
     2641 gttgcccggt aattaacttt tcatcctgcg gtaaggcggc ggcaatcagc cgcccgggga
     2701 gcaacagagt tgccactaac gtcagtaaga aacagaggtt tctcataatt atctccatgc
     2761 gaaaaccggg cgaatttacc cggttaagta aaatccgaac tattaaaatt tcatactgac
     2821 atcaagccag aaagtacggc cactggcgta ggtcgccaca ccggtgttcg ttgtatcaac
     2881 agcggttttg ctatccagta cattgagaat atcggcactg atcgtcaggt tttgttgttt
     2941 cagaaattgt ggcgtccagg acaagcgggt gtcccaggtc aggctgctat ccagtttttc
     3001 gtcaacatac tgcttgtaat ctgaatattc gctgatgtat tgcgcatttg tcttaccaag
     3061 gataatgcga gctttacgcg cttcttgcca ggccagcgta ttcgcccaca ctaacccgct
     3121 cggttgatgc gtgaaatcca tgtttaagga gatctttaat gggttattaa aatctgccac
     3181 tggaacgcta tcgtaagaga ccagattacc gttataaacc acctggttat caccggtatt
     3241 gctctcctca taaccattgt ttaacgacaa gttgcccttg ctcttgatat agctaaagac
     3301 aatttgtggg ttaatatcta cctggcggat atgcaggggt tcggccagtt caaaactgag
     3361 gctgaacgaa tgcgttttgg ttttgccatc gctgttatat tcagtaatgg tggttttagt
     3421 cgcgctgtcg gtacgactgc ttttgctgat ttgatcatgc gcttcacggt aaacatagtt
     3481 tgcacgcgca ataacgttct taccgatttt ctgctgcaat cccattgcca gttcatcgtt
     3541 ataaggcgtt ttcaaatcct gataacgcgt cagagtttta ttacctgata ccgattccgt
     3601 ccagctattg cggatatcac gtaatcccat atcaagaata ttcccgccat agtaacggtt
     3661 ataacctgct gtaatcattg aggtttgatt agcaaaaata tcccattccg tcataaagcg
     3721 cggggagata ttgtggtttg acagatagtt gtcatagtca taccgcacgc cgggcattaa
     3781 tgacacatta cgccagctaa tgcgatccgc catatacagc gtgtagttgt caattccctg
     3841 gcggccttta cctttatggt aaatggtatg gttagttttc tttccggcag cattaatcac
     3901 ataggattca gactggttat ggcgttcagt ccacgcatcg gaatagatgt attccgcgcc
     3961 gaagtagggt tgatgcgaaa cattacccac ggcgaatttt tgccagtcca ggcgtgtttt
     4021 gaaggtgtaa ttatctacag cctgggaaat gtgtcctaat ccgccacggg tgcaacgccc
     4081 tgtaatatca ccatatgtac atgaaagttc ggtgtaccag atgtcatgat cgtgacgggt
     4141 ataatcacta atatgatccc aaccaacggt ggtacgcagt ttggcccatg cgagctgcgt
     4201 atccatatcc catgccagac cataggattt attacccatt tcgcgatcag actgcgggaa
     4261 ggtgctggta ttataatcac ggctggagtc ggtatatttt aaggttaaat cgtgggtaaa
     4321 gcggtcgctg gcaaaccagg taaatttgct caatgcagta tcgataacgt ttttatactg
     4381 tgcccgaccg gcgacaatgc cgtcattcga aacataatcc gcgcgggtga tatcagactg
     4441 gcggcgcgat aaaccggcgg taacgccgaa gttatcagcg agttcctgat taaacgacaa
     4501 ggtataaaag ttctttttaa aatctgggga gtaataagta cttcctgaag agccttgatt
     4561 aaatgcgctc ttgttattct catcgatatg cgatgttaac cagtccgaac gcgtagtgcg
     4621 ataacccaat ttcaccttgc tatcatcagc gttgaagcgt ttgatctttg catcaattac
     4681 cccgccattg aagcgaccaa attcaaccgg cacaaaactg tcataaagcg tcacattgtc
     4741 cagtaagctg acatcaagat aatacccctg tgacatcccg ctaatattgg ttgcactact
     4801 ggcatcggac tcattcgctg ggttcaggtt attagttgca ctaataccgt caatcaaata
     4861 ggcattctgg tagggcgacg caccgtgaat agagattttc tcagggcgaa tatctccctg
     4921 gttcaacgag gtactttgcg ttgaatccat gcgtaccgca gggttggttc tcagcagatc
     4981 gctgatattg ccattcccgg tcggtaaacg ctcgatgctt tcactggtgt aatgcgtgtt
     5041 gccgtttacg ggtacaggga caggagcata gaccgtcatc tcttcaaaaa aatgcatgta
     5101 catgtttttg tcatcgacgg cctgcgccac atcagcgcca cataaaatga cgccaggaat
     5161 aagaactcgc ttcatttatt tatatctccg tacagcaggg cttttattgt tgttataaaa
     5221 ccgtgctaat gtcacaaata tcatcagcca ggttccagac accgggttga tgtgtaacca
     5281 taatgacgcc gcttgtgggt agtttttcac gcactaaacg cagtaagcgt atagcctctt
     5341 gttcctcaag atgagaggta gtttcgtcaa gaaatatcca tttcggacgt cgtaaaatta
     5401 atcgtgccag ggcgatacgt tgtttttcgc cgctggaaag aatatctccc cagcgatcgt
     5461 ggtcatgaat acgcgcagcc aatttcccca gaccaacctg atgcagtact tcgctcaacg
     5521 atttatcgtc tacgggcagg ggaagtgctt tacaaataat ctctttcagt aagccgcttt
     5581 tgattaacgg tgtttgtgac acataccaac tgtcagcagg agaagaaata tcacctttaa
     5641 accacggcca gcagtgggat aatgttttaa gcagtgtggt ttttcccgca ccagagtagc
     5701 ctttcagtaa tagccatttg cctggcgaaa catgaaagtt caagttctct aatatgatct
     5761 tattatcagg cgtacgaata ctcgcattag ccacttgtac cgcatgttgg caatttttag
     5821 gcttattcgt agggcgctgt tcagtgagtt gatggaactc atacaagcga tcgataaccg
     5881 cagccagctc agcaagttcg tcatatttat aaataaacca gcttaaattg ttcgatacta
     5941 gcataaatgc ctggcgcgat ttcattagtc cgcccagatt gatctgcccg ctaataaact
     6001 gcggcaataa cagaaagtag ggaagaacgc taagcgagcg cgaataaata ttttgccagt
     6061 aatcaagcca ccgctgacga ttcattaaac gatgccagtt ctctttaatg gtatgaaaat
     6121 tgtcgcgcaa ctcctggcgt tgtagagact cagcattgct tagcgcaatc agttctgcct
     6181 gcttattatg ctgcacaaga ttagttcgaa atgtcgcttc gctacgttgt ttttccacat
     6241 taagcggacg aatacgttta cccaccttat gggtaaataa agttccaccg atcacaatga
     6301 gcacaacggt atagaccata tacccttgga tattccattc tgttccacca acagtgaatg
     6361 agagcgtacc cgcgctttgc caaagaataa cggtaaaggt gatcagcatg ctaagtgact
     6421 ggatgaagcc aaatgaaagg ctgagcgttt tgctgatcag taagagaatg tcttcagcga
     6481 tacgttggtc agggttatct gtatttttat gctcaccgta gatctgcgtg aagtagtagt
     6541 ttttatctgc aaaccaccga ttaaggtagt aatctgttaa ccattcgcgc cagcgaatag
     6601 tcagcagttt aattaaccat gttttgttta cggatattaa tacaaagatc cccagcaggg
     6661 cagggaacca aaggacaagc tgccaaagct tatcggtttc tttctggctt agcgcattga
     6721 aaaaatcatt attccagtcg tttaaccaga cctgaatttt aaccacgcca aggatcatgg
     6781 cgaggataat aatgattaac aacactgaag ttttattatt cttacgcagc caaaagggct
     6841 tcagcagaca gagatacttt gctatcaaca tacaaaacgt aatgggaatg gttatcatta
     6901 gcgaaaattg attg
//
LOCUS       DAFNBJ010000217         6887 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000217 DAFNBJ010000000
VERSION     DAFNBJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.217"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(248..733)
                     /gene="sixA"
                     /locus_tag="L2X49_004470"
     CDS             complement(248..733)
                     /gene="sixA"
                     /locus_tag="L2X49_004470"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBN4799449.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(936..3080)
                     /gene="fadJ"
                     /locus_tag="L2X49_004471"
     CDS             complement(936..3080)
                     /gene="fadJ"
                     /locus_tag="L2X49_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBN4799450.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPAIERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3080..4390)
                     /gene="fadI"
                     /locus_tag="L2X49_004472"
     CDS             complement(3080..4390)
                     /gene="fadI"
                     /locus_tag="L2X49_004472"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBN4799451.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKIV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRVKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4571..4855)
                     /locus_tag="L2X49_004473"
     CDS             complement(4571..4855)
                     /locus_tag="L2X49_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBN4799452.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5227..6567
                     /gene="fadL"
                     /locus_tag="L2X49_004474"
     CDS             5227..6567
                     /gene="fadL"
                     /locus_tag="L2X49_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBN4799453.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1721 a         1845 c         1630 g         1691 t
ORIGIN      
        1 aactctattt tctctattat ctgtattaat aaatgacaat gagtgtcaaa cagacattat
       61 tcatttcaac atgtaacttt gggcaaaatc aagatacgcg cagaagaata atttccgaat
      121 gaaagtagaa agtgttttat ttatagatgg ggagagtctt cgatagtagt gaggttgaca
      181 gatatccgct gcctgatgag caattatgcc caatgaatct acctcattgg gcataatttg
      241 gaactcatca gatagctttt gccatcttca gattacacgg gctcatctgc cagttgaatg
      301 tgccgtttcc gctttcatca agcgttacgc tggcaatggc cgaggtggta aacataggcg
      361 gcgtttcgcc cggacataac tcggcaacca gatacccgac taacggtaag tgagagatca
      421 ccagtaccga agcaacacct tcattggtca acgcctgcaa ataggcactg accagaccaa
      481 catcgccgca gggcgttaac tccggcagaa cttctgcact ggaaggcagg ttcagacaat
      541 cccctacttc ttccagtgtt tgctcggctc gcaggaacgg gctcaccaga acgcgttcga
      601 tttccacttt ttgacctttc agccagttcg ccatcaggcg agattcgtca caaccattag
      661 tggtcagagg acgaaccgaa tcactggcgg catcgagggc tgcgtcgccg tgacgcatga
      721 taaaaacttg catattgcac cgcttttgtt aaccagattc gcccattttc tttcatctga
      781 aaccagagaa agaaaacggt ggccggcatt gtgccttatc cattcaccga atgaaacgct
      841 gttttttacc tcaatggcgt aagtatagtc aatatgcgtt tacattttga ccaacactcc
      901 gccattcagc gcggattcat atagctttga ccttcttatt gcaggtcagt tgcagttgtt
      961 ttccaaaaac tttccccacg cgcgcccatc tcgaccaaac gctcgcaagg ggtaaaacgg
     1021 gaaccatact gcgtggccag tcgttgcatt attgcaacca cttcgcccgc gccgagagaa
     1081 tcgatatagc ggaacggtcc accgagaaat ggcggaaaac caatgccaaa taccgcacca
     1141 atatccccgt cacgcacgct acggataacc tgctcatcaa cacaacgtac tgcttcattc
     1201 agcatcaaca tcacacaccg ttcagcaacc tgcggtgcgg agagtcgccc ctgcccttgt
     1261 gcgccaatga gcgggtaaat ggcaggatcg acctgttttt tgcttttacg ccctttctga
     1321 ccataaagat agaaaccccg accatttttt ctgcctttgc gatcgtcgtt caaaattgaa
     1381 gaaacaacat ttgcaggcgc gctaaaacgt tctccataag cggcttccag tacaggaata
     1441 attttagtcc cggtgtcgat tcctacctca tccaaaagtt ggattgggcc taccggaaaa
     1501 ccaaatttca ctagcgcagc atcaatgtgc tctacccgtt caccttcggt cagcatgcgg
     1561 atagcttcat taatgtaagg cgctaagatg cgattgacgt aaaacccggc tttgtcacgc
     1621 acgacaatgg gcgttttgcc ctgttttttc gccagtttta ccgtagtggc gatggtttgc
     1681 gctgatgtac ccgcatgagg aataatctcc accagcggca ttttttccac cggactgaag
     1741 aaatgcaggc cgataacttg ctcaggtcgc gtggcgtgag cggcgatatc accaatcggc
     1801 aaagatgacg tattcgaagc aaagatggta tgagcggcgc aattttgctc cacttccgcc
     1861 accatctgtt gttttaattc gagattttca aacaccgctt caataatcag atcgcgatgg
     1921 gcaaagccgc gatagtccgt cgttccggag attaatgcca gctgtttgtc acgttcgctg
     1981 gctttgagat gacgacggcg aactttgccc tccagctgat cccaactgta cttcagcgca
     2041 tgatttatgc cctgcgggtt gatatcttta attctgaccg gaagccccgc tttacaagca
     2101 gtgacataag caataccgcc gcccatcaag ccaccaccta aaattcccac gctgtttaat
     2161 ggcgcaggcg gtgcatcact accgggatct ttcttcacgt ccgtactggc aaaaaagata
     2221 ctacgcaaag cttgcgattg tggcgtcatc gccagttcgc caaacgcccg agcttcggcg
     2281 tcataaccgc tgctggtgcc ctgcgctaat cccgtttcaa caacctccag gatgcgttct
     2341 atcgccggat aattgccttg agttttgtgt tctgttttct tgccgaccat tttgaacagc
     2401 agcgcacgac ctaacggccc cgccagaata cgctcgcgta caggtagagg gcgggaagat
     2461 gggcgatcct tcttcgccag ctcaacagcg gcttccagca aaatggagtg cggaacaacg
     2521 tcatccacca gccccagctt taatgcctgt ttcgcccgaa gttgttttcc ggtgagaatc
     2581 atctctaatg ctgtactgac gcctatcaga cgcggtaaac gctgggtgcc gcctgaaccg
     2641 ggtaacaatc caagttgtac ttcaggcaaa ccgagcaccg ttttaggatc gtcagtacaa
     2701 acgcgaccgt ggcacgccag cgccagctcc agcccaccgc ccaggcaagc gccatgaata
     2761 gccgcgataa ccggaatggg caaagcatga atctccgcca tcaactgttg gccctgccgc
     2821 gccagtgctt ccgcttcttg cgccgttttg cagttgccga tcatgttgat gtctgcgcca
     2881 gcaatgaagt tgtccggttt agcggagaca aacaccacgc ctcgcaactc tttgttttca
     2941 cggagttgct taataatggc gcgcacctgc gaggcaaact ccgccttcag ggtattcatt
     3001 ttctcacccg gtacgtcgat ggtgataacg gcaatgttgt ccagacgaac attaagggta
     3061 aacgctgatg tcatttccat tattccgcct ccagaaccat tgccgcgcca agcccaccgg
     3121 cagcacaggc ggtaactaaa ccaaatccac cgccgcgacg gcgaagttca tgcaatgtct
     3181 gggtaatcat ccgcgcgccg gtcgccgcga agggatgtcc gtaagcaatc gaaccgccaa
     3241 gcacgttaaa tttgctatcg tccacttcgc cagtggcatg tgcacgcccc agtacttcac
     3301 gagcaaaacg ttcactaccc agcaactgaa tattcgccag tgtctgagct gcaaaggctt
     3361 cgtgcatatc gatcaatgtc agttcgctca tcgtcaaacc ggcacgttcc agcgccagcg
     3421 gtgttgacca ggctggaccg agcaacatgt cctgccagac atcaatcgca gtaaatgcgt
     3481 agctgcgcag ataccccagc ggcaccagcc ctaattcttt cacccgggat tcagtcatca
     3541 ggatcaccgc tgccgcgcca tcggtcagcg gcgtactgtt tgccgccgtt accgttccgt
     3601 gtttgcgatc aaacgccggg cgcagctttg cgtaatcggc aagcgaggaa ttaccgcgaa
     3661 tattgttgtc ttcgacaagc ggttgtttat aaggagggat aaaggcagtc atcacctctt
     3721 ctttgagttt tccgtctgac catgcctgag cggcacgctg atgcgaacgg tgcgctaatg
     3781 catcttgctg ttctcgggtg atgccgtagg ttttcgccat ttgttcggcg gtgtcgccca
     3841 tccgcaagcc agtagaatat tctgctaccg caggcggtac gggcattaag tcgcgcaaac
     3901 gcaggcgaga gaagagtttc aggcgctggc tcatggttcg agctttgttg acatcaacca
     3961 gcacgcgcgc cagtttttta ctgacgccaa ttggcaatac ggaagaggaa tctgccccac
     4021 cggcaatccc cgctcggata gttcccgcca tcaggctttc tgcgacgttt gcaaccgcct
     4081 ggaaactggt agcgcaagcg cggctgacgc tgtaagcatc ggtatgcaca ttcattccgg
     4141 taccgagaac aatttcacgc gcaatgttgg gggcttcagg catttgtacg acctgaccaa
     4201 agaccagctg ttcaatcact tcagcgggga tctcgctgcg tgccagcagt tcgcctacca
     4261 caatcttccc taaatcaacc gcgggaatgc catgaaaagc cgtcgcctga cgggcaaaag
     4321 gcgtacgtaa accgctaaca atggcgatac gatcgccctg gcgggtaacc agcggtaaaa
     4381 cctgacccat aacactcccc tgtaaaaaaa taaataaagt ggtctgacct gatcatagtc
     4441 ttaaccattt ttttacattt agccaagtgg agaaaaggga aagtgggagc tatgacacag
     4501 agagaaaaga agaagagaaa agaaaacgcc cctgccagca actgacaggg gcgtacgtac
     4561 ccgagaggaa ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag
     4621 tgaaatcgat atccaggcgc acaccgtcag actcttcagt gaaagtcggg gtgattttgc
     4681 acggttcgga ttccacgcta cgggcttttt cagtcagcgc cgccagcgtt tgttctgctt
     4741 ctgcgcggct tgcaaacacg cggctgtaag acgcggtgca gtcggagttg tccataatgg
     4801 tgccaacatc catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg
     4861 atttcctctg tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg
     4921 gaaagtgctg ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta
     4981 aaaatgatct aaaacaaaat tcacccgaat ccgcgagtgc gcaacctcca aattttgcca
     5041 gctggatcgc gtttcttaga tcatatttga aaaaagatag aaacatactt gcaacattcc
     5101 agctggtccg acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta
     5161 cacttcgcgc tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt
     5221 atggtcatga gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt
     5281 atctccaccc aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg
     5341 ggccgggctt attcagggga aggcgcaatt gccgatgatg caggtaacgt tagccgtaac
     5401 cccgcattga ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgac
     5461 ccggatgtga atatcagcgg aacgtctccg tctggtcgta gcctgaaagc cgataacatc
     5521 gcgcctacgg catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt
     5581 tggggcgctt ctattacctc taactatggt ctggcgacag agtttaatga tacttatgca
     5641 ggcggctctg tcgggggtac aactgacctt gaaaccatga acctgaactt aagcggtgcg
     5701 tatcgcttaa ataatgcatg gagctttggt cttggtttca acgccgtcta cgctcgcgcg
     5761 aaaattgaac gtttcgcagg cgatctgggg cagttggttg ctggtcagat tatgcaatct
     5821 cctgccgggc aaactcctca agggcaagca ttggcagcta ccgccaacgg tatcgacagt
     5881 aataccaaaa tcgctcatct gaacggtaac cagtggggct ttggctggaa cgccggaatc
     5941 ctgtatgaac tggataaaaa taaccgctat gcactgacct accgttctga agtgaaaatt
     6001 gacttcaaag gtaactacag cagcgatctt aatcgtgcgt ttaataacta cggtttgcca
     6061 attcctaccg cgacaggtgg cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa
     6121 atgtgggaag tgtcaggtta taaccgtgtt gatccacagt gggcgattca ctatagcctg
     6181 gcttacacca gctggagtca gttccagcag ctgaaagcga cctcaaccag tggcgacacg
     6241 ctgttccaga aacatgaagg ctttaaagat gcttaccgca tcgcgttggg taccacttat
     6301 tactacgatg ataactggac cttccgtacc ggtatcgcct ttgatgacag cccagttccg
     6361 gctcagaatc gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg
     6421 acttacgcat ttaataaaga tgcttcagtc gacgttggtg tttcttatat gcacggtcag
     6481 agcgtgaaaa ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc
     6541 ggtactaact ttaactacgc gttctgataa cacgttcgcc tggataaagt cacctgcata
     6601 gcaggtgact ttaactcccc cacttcaccg aaagtagtgt cctcattgct tacctcctga
     6661 gttttgcaaa cagcctgatg gcagcttgcc ctttcaaatc aataagcggt atccacagac
     6721 aaacctgaag ggaaaggcat attttcaggc gttctgctcg tccttctcaa agagtttact
     6781 tttctgaatt tccaggatac tcaccccccc cctggctatt gtgcgctcat acactcaaat
     6841 taaagatagg ttctaaataa atgagcgttt tttgatagtc tatttca
//
LOCUS       DAFNBJ010000218         6879 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000218 DAFNBJ010000000
VERSION     DAFNBJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.218"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(80..1729)
                     /gene="pgi"
                     /locus_tag="L2X49_004475"
     CDS             complement(80..1729)
                     /gene="pgi"
                     /locus_tag="L2X49_004475"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBN4799454.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNTKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRD"
     gene            2253..3602
                     /gene="lysC"
                     /locus_tag="L2X49_004476"
     CDS             2253..3602
                     /gene="lysC"
                     /locus_tag="L2X49_004476"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBN4799455.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAAVQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3747..4673
                     /locus_tag="L2X49_004477"
     CDS             3747..4673
                     /locus_tag="L2X49_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBN4799456.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNHIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYNKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLADKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4709..5500)
                     /locus_tag="L2X49_004478"
     CDS             complement(4709..5500)
                     /locus_tag="L2X49_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBN4799457.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5506..6633)
                     /locus_tag="L2X49_004479"
     CDS             complement(5506..6633)
                     /locus_tag="L2X49_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HBN4799458.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGLKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1697 a         1782 c         1646 g         1754 t
ORIGIN      
        1 ccgttaaatc acagacaagg ggatttatct gataaaacaa tgccgacata acgtcggcat
       61 tgttattaag gtttttaaat taatcgcgcc acgctttata gcggttaatc agaccattgg
      121 tcgagctatc gtggctgctg atttctttat catctttcag ctctggcaga atacggtttg
      181 ccagttgttt acccagctcc acgccccact ggtcgaaggt gaagatgttc aggatcacgc
      241 cctgagtaaa gattttgtgc tcatacagcg caatcaacgc ccccaggctg aacggagtga
      301 tttcacgcag caggatggag ttggtcgggc ggttaccttc gaacactttg aacggcacca
      361 cgtagtcaag cgttgccgga tctttaccct gatcgcgata ttcctgctca accacttcgc
      421 gggatttacc aaacgccagc gcttcagtct gagcaaagaa gttagacagc agtttctggt
      481 ggtgatcaga gagcgggtta tgggtgatag ccggagcgat gaaatcgcac ggtaccattt
      541 tggttccctg gtggatcagc tggtagaacg cgtgctgacc gttagtgcct ggttcacccc
      601 agataatcgg gccagtctgg taatccacaa cgttaccgtt acggtcaaca tacttaccgt
      661 tggactccat attgccctgc tggaagtacg ccgcgaaacg gtgcatatac tggtcatacg
      721 gcagaatcgc ctcagtttcc gcaccaaaga aattgttgta ccagatgcca atcagcgcca
      781 gcagtacagg caggtttttc tcggcaggcg tggtggagaa atgcttgtcc atcgcgtgtg
      841 cgccagaaag cagttcaacg aagttatcaa agccgatgga gagaacaatc gacaggccaa
      901 tcgctgacca caaagagtaa cggccgccga cccagtccca gaactcgaac atgttggcag
      961 tatcaatacc aaactcgcca acggctttgg tattggttga gagcgccgca aagtgtttcg
     1021 caacgtgttt ttcatcacct gccgctttca ggaaccagtc acgcgcgcta tgggcgttgg
     1081 tcatggtttc ctgagtggtg aaggttttag atgctaccag gaacagcgtg gtttccgggt
     1141 ttactttttt cagcacttcc gcgatgtgag tcccatcgac gttagaaaca aagtgcatgt
     1201 tcaggtggtt tttgtacgga cgcagtgctt cggtcaccat gtacgggccg aggtcagaac
     1261 cgccgatccc gatgttcact acgtcagtga ttgctttgcc ggtatacccc ttccactcac
     1321 cggagataat cgcttccgag aaggttttca tcttctccag caccgcgttg acttccggca
     1381 ttacgtcttt gccatcaacc agaatcgggg tattgctacg gttacgcagc gctacgtgca
     1441 gcacggcgcg gttttcagtg cggttgatct tctcgccaga gaacatcgac ttaatcgcgc
     1501 ccgccagatc gcactctttc gccagatcct gtaatttcgc cagcgtctct tcagtgatgc
     1561 ggtttttgga gtaatccacc agcatctgat cgtcgaatgt tgcggagaac ttagaaaaac
     1621 gatcgccgtc tttagcaaaa agatcggcga tcgtaacgtc tttcatttca tcgaagtgtt
     1681 tctgtagtgc ctgccaggca gcggtctgcg ttggattgat gtttttcatt agcaatactc
     1741 ttctgatttt gagaattgtg actttggaag attgtagcgc cagtcactga aaaatgtgat
     1801 ggttttagtg ccgttagcgt aatgttgagt gtaaaccctt agcgcagtga agcatttatt
     1861 agctgaacta ctgaccgcca ggagtggatg aaaaatccgc atgaccccgt cgttgacaac
     1921 cgccccgctc accctttatt tataaatgta ctacctgcgc tagcgcaggc cagaagaggc
     1981 gcgttgccca agtacggtgt tggaggagcc agtcctgtga taacacctga gggggtgcat
     2041 cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct gaatcccctg
     2101 ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca cttctgggtg
     2161 aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc ttgtgccaag
     2221 gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc tccaaatttg
     2281 gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt gtgctttctg
     2341 atgccaacgt gcgtttagtt gtcctctcgg cttctgctgg tatcactaat ctgctggtcg
     2401 ctttagctga aggactggaa cctggcgagc gattcgaaaa actcgacgct atccgcaata
     2461 tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa gagattgaac
     2521 gtctactaga gaacattact gttctggcag aagcggcggc gctggcaacg tctccggcgc
     2581 tgacagatga actggtcagc cacggtgagc tgatgtcgac cctgctgttt gtcgaaatcc
     2641 tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg cgtaccaacg
     2701 accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc gcggtgcagc
     2761 tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt agcgaaaata
     2821 aaggtcgtac aacaacgctt ggccgtggag gcagcgatta tacggcagcc ttgctggcgg
     2881 aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc tacaccaccg
     2941 atccacgcgt ggtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc gaagcggcag
     3001 agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc gcagtacgca
     3061 gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt acgctggtgt
     3121 gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc aatcagactc
     3181 tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg gaagttttcg
     3241 gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa gtgagcgtgg
     3301 cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg acgcaatctc
     3361 tgctgatgga gctttccgca ctgtgccggg tggaggtgga agaaggtctg gcgctggtcg
     3421 cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta ttcggcgtac
     3481 tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac ctgtgcttcc
     3541 tggtgcccgg cgaagatgcc gagcaggtgg tgcagaagct gcattttaat ttgtttgagt
     3601 aatatttccc tactggcgta taacgctcac cgattgttat acgccatcct cactgaaaac
     3661 atagattaaa taatatctga aaagcaaaat cactcctgta gaacagtatt ataatcaccg
     3721 aaaaatatca tttaacagga gtgttaatgg ctactttaat ggaaaaagat gcacttttaa
     3781 acggagccag tcagtgcatc gcgtttttat caaatatcat tgataattgc tcagtttcgt
     3841 cgcatcagga ttcaggcgat gctctcaagc gattagtcag ctatcgcgat tatctttatt
     3901 ctacccccgc tgaacttgtt gattttacac aaggtaagat tctccttcag caggtaagaa
     3961 ctcaatatca gcatgaattc aacaatacta cgcattcaga aaataaagca tcttttgatt
     4021 ctatttggca gcgtctgaca aatcatgaag tcacaccgca acagcatccc attggttttg
     4081 ttttaggtgg gcaacccgga gcaggtaaat catctctgat tgaattagcc aaaagagaga
     4141 caaaagataa tataatgatt attaacggtg atgattttcg atttctacac cctgatttta
     4201 atcatatcta ccaaaattat ggggatgatt ttgttaccca tacagcgaaa ttttccggcg
     4261 aaacggtcga acgtgcaatt gagagagcga tagtcagtaa actcaacatt gttgtcgaag
     4321 gaacatttcg taatgccgct acgccgttac aaacattaaa aaaactcaaa gatgcaggct
     4381 accaaacaga agtcatgata aaaacaacct cagcggcatt aagctgggaa agcaccaatg
     4441 aacggtataa taaggacaaa gaggcgggaa atattgcccg aaaagtggat aaaaaccacc
     4501 atgatatcgt caccggtttg ctggcagaaa atgcccgcaa ggtgtttgct tcaaacctgg
     4561 cagataagtt tgcggtgtat tcgcgtgaga agatgatatt tagttcacaa gcggcaacga
     4621 atgacgatat tgcaacgctt atacagaatg aaatcagcgg gaatacacaa taataataag
     4681 agctgatggc gtgaaataac gccgggcttt atgcccggcg cattaccgtc tggtacatta
     4741 agctatccag ttccttaacg tccccttctc tgctatccgg taacagcggt ggaatttccc
     4801 cacgcgccag ttccgcacga ataaacgcat gtaacaatgg acggcgcgga ccatattccg
     4861 cttctcctgc gcgctgttta cgttccagta attcgtctat ttctgcacgt aacggcgcat
     4921 caagttcgct tcccgccagt aactcagcaa agcgcattgg cggtacgcct ttacccgctt
     4981 cgacccagcg cacggccagc agcggacgca gcacgtaaaa gtattttttc agtcgaactt
     5041 cgtcgccctg caaatatccg gggaagtttt tctgcgccat cgaatagtaa tgccagcgcg
     5101 cacgcagcgg agaaaaccac attgggacca ttgccttaaa cgccgttatc gtttcttcat
     5161 cttgttgata aaccaccgga gaatccagcc attctatcag cgtcgggttc gcgcctttca
     5221 ataatcccag cgccttacgc cactcccaac cggaaacatc taactcgtcg tcgatgggca
     5281 gctcaataac atcgcgcggt gactctactc gcagatacca ttccagcggg tgaacataca
     5341 aaaaccgtac atcgtagtcg ctgtcgggcg aggcaaatcc ccagccgcgg ctccctgatt
     5401 cacaggcgta cagtactttc acatcatacc gccgttcaat ttcttttaac tgctgcgata
     5461 ccctttctcg catcgcagca ctgactccat ttaatggcat gttactcacc ctttgatatt
     5521 aaccaaccca tgcagaacct gatccagacc gccaaataca gagatgttat cgatccgttc
     5581 agccactctc tccagcgttt ccagttcttt taagcgtaac gctaccgggt tgttttccat
     5641 gactttagcg gtattgagta acgaacgcgt cgctgccgtt tcttcacgtc ggcgaatcac
     5701 attggcctgc gcggatttct ccgcctcaac cagctgcgca aggatatttt tcatatcgcc
     5761 aggcaggacg atatctttca accctaacga ggcaatctcc ataccaaatg gcaacatgcg
     5821 cgatttcacc tgttcgctga caacgtcatc aatgacctgt ttatcttcca gcaattcatc
     5881 caacgtacgc gtacctaccg cttcacgcag ggcaaattgt aattcccgat acaaatgatc
     5941 aatcggcttc gttaactggc taaatgccaa taacacgtcg ctgtagcgcc agttagcggc
     6001 gaggttaatc cgcagattga ccttatcttt cgttaagatt tcttgcccgc tgacttccag
     6061 tacctgcaag cgggtgtcaa ccacttcagc gtcaaccaga tggttaattt tccagtatgc
     6121 ggtcagcccc ggcggtaaca gcgcctgcgt ttcaccatcg attttcagca cgcctacatg
     6181 ccaggccgga acctgcaccg ttagtattgc atcgcggcct ttaactgccc ctttgcgccg
     6241 tggttgcaat accgcattca tcacctccgt ttgtacctgg acttgctgcg tattcatccg
     6301 caccagtgtc aaatcatcct ctacacgcca gtacaaacgg cgcgttgatg gcggcaggat
     6361 ctcctgcaaa ataccgtcca tatacaacgc cccggcttca atctccgaca agtctgccac
     6421 caggcaatat ttctccaccc aatcaggctg aaagcggcgc aaataatccg ccagcacgtc
     6481 gggaacttcg ctgccatcga gcgtaatcag caagacttct ggcgtattta accagggtaa
     6541 aagatgctcg cctgcatgta gcactttgta gtaatcgccg tttctggaga gtaatgccag
     6601 ctgatctttt cttacgctta tttttttaat cattatctat tccttgctta acagccgtcg
     6661 cggaagcaga cacgaagcga caggggaatt cccttttgcc tgttacccgg catctttatc
     6721 ggcgacgtta tcggggatcg ctgactgcgc aatccctcac tttttattta ataagttaaa
     6781 accagtattc atttcaggaa tgaatgtggg aatcgaaccc ataattggcg tcaaaggctg
     6841 acggaacacg cccggaaaac agcctggccg ttcctggtg
//
LOCUS       DAFNBJ010000219         6849 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000219 DAFNBJ010000000
VERSION     DAFNBJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.219"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(137..805)
                     /gene="artM"
                     /locus_tag="L2X49_004480"
     CDS             complement(137..805)
                     /gene="artM"
                     /locus_tag="L2X49_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBN4799459.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(805..1521)
                     /gene="artQ"
                     /locus_tag="L2X49_004481"
     CDS             complement(805..1521)
                     /gene="artQ"
                     /locus_tag="L2X49_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBN4799460.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLI
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1528..2259)
                     /gene="artJ"
                     /locus_tag="L2X49_004482"
     CDS             complement(1528..2259)
                     /gene="artJ"
                     /locus_tag="L2X49_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN4799461.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2277..3005)
                     /gene="artP"
                     /locus_tag="L2X49_004483"
     CDS             complement(2277..3005)
                     /gene="artP"
                     /locus_tag="L2X49_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBN4799462.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3223..3738)
                     /locus_tag="L2X49_004484"
     CDS             complement(3223..3738)
                     /locus_tag="L2X49_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBN4799463.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKKGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3864..4187
                     /locus_tag="L2X49_004485"
     CDS             3864..4187
                     /locus_tag="L2X49_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBN4799464.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4184..5014
                     /gene="amiD"
                     /locus_tag="L2X49_004486"
     CDS             4184..5014
                     /gene="amiD"
                     /locus_tag="L2X49_004486"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBN4799465.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5011..6024)
                     /locus_tag="L2X49_004487"
     CDS             complement(5011..6024)
                     /locus_tag="L2X49_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBN4799466.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6123..>6849)
                     /locus_tag="L2X49_004488"
     CDS             complement(6123..>6849)
                     /locus_tag="L2X49_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBN4799467.1"
                     /translation="PLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTALRAL
                     IPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSAS
                     LAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWK
                     VIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMI
                     PAHLFIFRGMAKQIARLAEQSTD"
BASE COUNT         1655 a         1800 c         1814 g         1580 t
ORIGIN      
        1 ataagaattt atgcactatg taggccggat aaggcgtcca cgccgcatcc ggcacaggca
       61 ccgtgctgat gtctgatgcg acgctggcgc gtcttatcag acctacaaac ccccccggcg
      121 aatgtacgca gctacattaa tttcgccgtt cgaatgccag tgctttgcgc tcgatcagac
      181 gcatcatcag cgtcagcagg ccgttgacga ccaggtaaat aatccctgcc gcaccgaaca
      241 ccattacatc gtaggtgcgt ccgtacaaca actggctgta tcccatcact tccatcagcg
      301 taatggtgta agccagagag gtacttttga acaccagcac cacttcgttg gaataagaag
      361 agagcgagcg tttaaaggca tacggcagca ggatcgccag cgtatctttt ttgctcattc
      421 ccagggcgct acaggactgc cactgacctt ctgggatcgc acgaattgca ccgtaaaaca
      481 gctgcgtggt atacgccgca ctgttcagcg ataacgcaat cagcgcacat aaccacggtt
      541 ctgacaacaa atgccacagt gccggatact cctgcaaagt cggaaactgg cccggcccgt
      601 aataaatcag gaagatctgc accagcagcg gcgtaccggt aaacagcgtg atataacccc
      661 gcaccagcca caccagcacc ggcgttttca gcgtcaggat gatggtaaaa atcaatgcca
      721 gaatcagtgc cacaatcagc gaggcaacgg ttagcgtcag gctggtgtgt agccctttca
      781 tcagttcggg taaatactca aacattagct gggcctccgc tcaaaacgtg tcgcgcgcag
      841 gtcaatgcgt ttgagaatgt actgactgat cagggtgatc accaggtaaa tcgccgccgc
      901 cacaatgtac caggtaaatg gttcctgggt acgagtagcg atgctttttg tttgcagcat
      961 taaatcattc acactaatca aactgaccag cgcggtatct ttcagcagca ccagccactg
     1021 gttaccgagg ccaggcagcg catgacgcca catctgcggc atcaccagac ggaaaaagat
     1081 agccgatttc gacagcccca gcgcctgacc agactcccac tgacccaccg gcaccgcttt
     1141 caacgcgccc cgaagcgttt gcgaggcata ggcggcatac agcagtgaca gagcgatgac
     1201 accacaaagg aacgggctaa cgtcgaagtt ctcaatgtcc atctgcactg ggatctgcac
     1261 gaacccaaga ttgatagtga agccatccga aagcgtcagc agcagctgcg aggagccaaa
     1321 atagataaac agcaccacca gaatttctgg caggccacgc agaatggtta ccagcgctga
     1381 acctgcccac gcgacaggac gccattttgc cgactcccat accgcaaaga acatcgccag
     1441 cgccagcccg acaatcaatg cacaaacggc aaggccgacg gtcatcccgg cggcgcttgc
     1501 taaaggaaaa aattcattca tcaggaatta cttctggaac catttgttgt agatggtttc
     1561 gtaagtgcca tctttcttca ctttttccag cgcagtgttg agtttctgct gcagctcagt
     1621 gttgccctga cgtaccgcga tgccgaggcc agtgccgaag taatctttat cggtcacttt
     1681 gtcgcccacc gccgccagtt tcgggttatc tttcagccac tcagtgacca ctgcggtgtc
     1741 accgaagacg ccgtcgatac gcccgttttg cagatccagt tttgcgttct ggtagctgtc
     1801 atacggaacg gtagtgattt ccgggtgctt atccataatg aatttctggt gtgtcgtccc
     1861 gttctgtacg ccgacttttt tgcctttcag ctgatcaaca ctggtgtatt tgccttgctg
     1921 acccacaaac agggcagagt tgtcatagta cggggtggta aacagtacct gcttttcacg
     1981 ctccggagtg atatccatgc ccgccatcac tgcttctacg cgacggaatt tcaggcttgg
     2041 gatcaggctg tcaaacgcct ggttagagaa agtacaggtt gcatcaatct ctttacacag
     2101 cgcctgtgcc aggtcgacgt caaaaccaac gatctggttg tttgcatcaa tcgattcaaa
     2161 cggcggatag gaggcttcgg tagcaaaacg aatggtttcg gcagctgtgg cggaaagact
     2221 aaaacctgca attaacgcgg caatcagaac ttttttcatt gttgttatcc cgaatcttag
     2281 tgagagagat agtttttaaa tgcttcggtt tgcggctcgg taaagcagct cgcgtcgcct
     2341 tgttctacga tatgaccatt ttccatatac accactcggc tggcggtttt acgcgccact
     2401 tcaacttcgt gggtgacgat cacctgggta atattcgttt ctgccagctc acgaatgatg
     2461 ctgacgattt gtgccgtaat ttccgggtcc agtgcggcgg tcggttcatc gaacagcagt
     2521 acctgcggtt ccatcatcaa cgcacgggca atagcaacac gctgctgctg accaccagaa
     2581 agatgcagcg ggtaacgatc gctataaggt ttgagacgca gacgttccag cagtttttct
     2641 gcacgggcca gcgcctgatc tttactcaac cccagtacac ggcagggcgc ttcaatcagg
     2701 ttttgctgca cggtcagatg cggccacagg ttgtattgct gaaacaccat gccaacgtta
     2761 cgacgcaaat cgcgaatcgc tttgtcagag ggtgttttgg tgaaatcgaa atggttgcct
     2821 gcaatgttga gcgtaccgga gcgcggcatc tcaagcagat tgagtacacg cagcagcgag
     2881 cttttacccg cgccgctggg gccaagtaac accagcgttt cgccctgtgg gcaatccagc
     2941 gtgatatcga acagcgcctg atgcgcgccg tagaagcaat taatgccgtt taattgaata
     3001 ctcattgaca ctcgtatact ggcagtctga tagctattga ggtcgaagat agtacctttg
     3061 acagaataat tatgcaatat ttctgcttta aaagttaaaa gcaaagcgca ttattcaata
     3121 aacatagcac aaaataacgg gggcggtggt cggcgagcat aaatgtcggc attcctcacg
     3181 aaatgccgga caatttacgg ggtttattgg ttgatcaagg cgttagcgat tctcgatgga
     3241 ctgacggagc gtacccgccg tggcatgaac gctgccgcct aaatagcgta catcgtcgat
     3301 cacccaacac tggccttcct gaatcattaa cacttcatcc tgccagctct gatcaccctt
     3361 ttttagatcc acacgcagag gaatatttcg cgcatcacga ttcgggattg ttgaggcact
     3421 ggcaacacgg gcgctctctg gcaaggttgt gcgactggag aatggatcgt tggtcagtag
     3481 ttcccgatgg ttattatccc gggaggcatc gctaagcagt gtcgccagtt tgtcgctcag
     3541 ataagggcgc aaggcggtga tgtcgttgct gcggtgcaaa atgcggtagt cataaaattg
     3601 ctgggccacg ttatccgggc ctccttcaac gcaaggacca ctgcgtgtgc cgttatcttt
     3661 ataagctgga gtgactgtgg tgcaggcact gaggagcagt gcgcagggga taagcattgt
     3721 caatttgctg tagcgcataa tgatttcctt ataagcgatc gctctgaaag cgttctacga
     3781 taataatgat atcctttcaa taatagcgta tcagtctgat aatgcttttg agatcgaagg
     3841 cttagcaaac aaggagatcg atcatgcaat tttctacaac cccaactctg gaaggccaga
     3901 ccatcgttga atattgcggt gtggtgaccg gcgaagcgat tttaggtgcc aatattttcc
     3961 gtgatttctt tgccggtatc cgcgatatcg ttggcggacg ttccggtgcg tatgaaaaag
     4021 aactgcgtaa agcacgggag atcgcctttg aggaattagg ctcccaggcg cgggcgctgg
     4081 gggccgatgc cgtcgtcggt attgatatcg actacgaaac ggtcgggcaa aacggcagta
     4141 tgctgatggt tagcgtcagc ggtacggcgg tgaaaacgcg tcgatgagaa gagtcttctg
     4201 gctggtcgct gccgctctgt tattggcagg gtgtgcaggc gaaaaaggca ttgtcgagaa
     4261 agagggatat cagcttgata cccgacgcca ggcgcaggcg gcgtatccgc gcattaaagt
     4321 gctggtgatc cactacaccg cagatgattt tgatagctcg ctggcgacac tgaccgataa
     4381 gcaggtcagc tcgcattatc tggtccctgc ggtaccaccg cgatacaacg gtaaaccgcg
     4441 catctggcaa ctggtgccgg aacaagaact ggcctggcat gcggggatta gcgcctggcg
     4501 cggggcaacg cgccttaacg acacctctat tggcattgag ctggaaaacc gtggctggca
     4561 aaaatcggcc ggagtgaaat attttgcccc gtttgaaccg gcacagattc aggcgcttat
     4621 tccactggcg aaagatatta ttgcccgtta tcacatcaag ccggaaaacg tagtggcaca
     4681 tgcggatatc gcaccgcagc gcaaagacga tccggggcca ttatttccct ggcagcaact
     4741 ggcgcagcag gggattggtg cctggccgga tgcgcagcgg gttaactttt accttgccgg
     4801 gcgcgcgccg cacacaccgg tagataccgc gtcattgctg gagcttttgg cgcgctacgg
     4861 ttatgacgtt aaacctgata tgacaccgcg cgagcagcgg cgcgtgatta tggcattcca
     4921 gatgcatttc cgcccgacgt tatataacgg cgaagcggat gcagaaactc aggcgattgc
     4981 cgaagcattg ctggagaaat acgggcagga ttagcgcggc agttttccgt ggtcgcgtag
     5041 ccaggcggca gttttctcga taccttcatc cagggtgatg accggctgat aacctaactc
     5101 ttcctgcgca cgcgtaatat ccagcgtaaa gtcaaaattc aacttggaga cgccgtagtg
     5161 ggtcagcggc ggctcttttg ctgacttgcg gcctaaacgc tccatgctgc gggcgatcat
     5221 atccagcatc gggtagggga cggaacgaat acgacagtca atattcaact cgtcgatcag
     5281 cttctgcacg atgctgcgca gtgtgcgatg ctcgccgttg gtgatgttgt acacacgccc
     5341 ggaaggtagc ttatcgcagg cttcctggct tgccagccac attgcgtgca cggcattttc
     5401 atagtaggtc atatcgacca gcgcactgcc gccatgcggt aacagaatac tgccgtagtg
     5461 gtgcatcata tgcgccagac ggggaataaa gactttatcg tgcggtccga acagactttg
     5521 tgggcgcaga atagtaaagc gcgtttgtgg attcgcctgc gaaagcatat tgatcacttc
     5581 ttcgctggct gctttgctgc gggcaaactc gttggcgaag cggtgagggc gaaaatcttc
     5641 tttaatatcg cgatggtggt gataatcgaa gtacagggag ggggaagaga tatgaataaa
     5701 gttacgcaca ccccaggcga cagcccattc acccaggcgg cgagtggcgc gaacgttagc
     5761 cagatcgaaa gcctgttgtg tcccccaggg tgaggtaaag ctggagcagt gccacagcgt
     5821 atcaatgccc gcgagcatca ctttagcttg tgatgaaacc agctcggtca gatccgccgg
     5881 aacaaactct gcgcccattt tttccagcaa tttgcccatt gcctcgttgc gaccggtcgc
     5941 tcgcacgctg atgcctttct ggcataaaaa ctctaccgcg tttcgaccta agccgctggt
     6001 ggcgccggta accagtacct tcatatcaat ccactgttgt tgagaaaata acgtgcgcat
     6061 tcttccgtga tttcccccat gatgcaatgg gaaacatgaa agaataacgc aggttttgtc
     6121 gattaatctg tgctttgttc tgccagtctg gcgatttgtt ttgccattcc gcgaaaaata
     6181 aacagatgcg cggggatcat caataaccag taaaacagcc ccggcatacc gtgcggatgc
     6241 cagaaagcgc ggacatcgat agtacgatag tcgcctttat cttccaggct aaaacacagt
     6301 cgtcccagcc ccggcgcttt catgccaaat aacaacgtaa gttgtttttc cggttcaacg
     6361 acaatcactt tccagctatc taccgcatcg ccagtctgta aatattcgcg ctccgggcgg
     6421 cctttcgcca gcttatgacc gatcgcgcgg tccatcaacg cccgtgtctg ccacaaaata
     6481 ttgccaaaga aataacgctc tttaccgccg atttggttca ctacctgcca taaagcagca
     6541 aggctggcgg acgttttaac ggtaaacccc gcctgtttgg caaaataacc gtactccggt
     6601 cgccagcggg caaaggcctg agcgtcgtag ccccagtcgc tggagttgac cagtttttcc
     6661 tcctctttca acgtgctacg taccgcgtca tcgaaagcga tcagccgttg tgggatgagt
     6721 gcacgtagcg cggtatcatc cgccagcaga tcgtgtttca gcccctgaat caacgccctg
     6781 gcggtggtgg gcggtacgga agtaatcaca ttgagaaacc acaccgaaat ccagcgggtg
     6841 gggaggggg
//
LOCUS       DAFNBJ010000220         6726 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000220 DAFNBJ010000000
VERSION     DAFNBJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.220"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..442)
                     /locus_tag="L2X49_004489"
     CDS             complement(<1..442)
                     /locus_tag="L2X49_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HBN4799468.1"
                     /translation="MQLNTRQERVYTLANILGSGKPVSAQSITATLACSEPTLSRALK
                     ELRETYAAEIKYSKAGHSYQMVNSGLLDKKTLKRMSEALDLNTDLKTHDQGARVILDK
                     DIKTSVSLSIRRRILRKIDKLAELSGTSRSEAVEKLVEMKIDEII"
     gene            complement(445..1668)
                     /locus_tag="L2X49_004490"
     CDS             complement(445..1668)
                     /locus_tag="L2X49_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBN4799469.1"
                     /translation="MGFRFRKSISIIPGVRVNLSNGAPSLSIGPRGASLSVGKNGTFA
                     NLGLPGTGLSYRTRIDRTARERVNTRHQADPGLKSELELVVEKLMSTVTVITNIHELS
                     PSPKGGNTWAMLETQYLALRQGSFSLPAPVRPNKPEYIPLPPEPDEHAGRGFLGGWFE
                     SDAARQERQNENLRRWQVTVADIERENELLKQRYEKQRIAWAEQYAEWQHQYQEHEKN
                     RTESVALAKEQFRSDARFFEHCLEEVFSQTEWPRETLVSFEVRPEESTVWLDVDLPEI
                     EDMPDKVYSVNARGTDINEKAMTQKAVRESYAKHVHGCLLRLAAIVFQTLPFEQVVIS
                     GFTQRVSKRTGYLEDEYIISWRVCRSEIELINFGNLRGLDPIEALGDRGLIRKMSATY
                     IFQPIEPLTHMAGTD"
     gene            complement(1679..2635)
                     /locus_tag="L2X49_004491"
     CDS             complement(1679..2635)
                     /locus_tag="L2X49_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBN4799470.1"
                     /translation="MKKQPNPWELGATLYMPATRKDIAEVVLEGKIPGLRSLVVCLED
                     AVSEHDIPLAIQNLSLFLERLRHARAVNDDEKYPLVFIRPRHPDMGRWLTANLDLSAV
                     DGFVLPKFTRSTLPVWWDIMAGTSLMMMPTLETEEVYDVIKMQALANELSSHDCRDRI
                     IALRIGGNDLMNVVSLRRPRHLTLYDTPMGYVIKMLVSVFAPRDFALTAPVCEHIDDH
                     GVLGRELSMDIAHGLVGKTAIHPDQISVIEEALKVSPGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAQGILGRGQVYGLTTGQNPEGIRLIPVSKQH"
     gene            complement(2635..3714)
                     /locus_tag="L2X49_004492"
     CDS             complement(2635..3714)
                     /locus_tag="L2X49_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBN4799471.1"
                     /translation="MSHFAPFSGSYKPDDVHFLLKPIVMEMTPVDLKEELIQSGKMHY
                     SDMLSQEPEPTRWHLDLFTRALDSGAARLAREVSYLARELARRAGDEPIVLVSLVRAG
                     VPLGVMLHQALRAMGKQSFHYGISIIRDRGIDAAALDFIESRHGTGGIVFVDGWTGKG
                     AITGELSRTLKDRPGYPEQPRLVVLADPCGCAWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWAEEGLHGCVVCDHLQSFECSQMLVDTVAHFREQLDGKPLPASGWDAGKNAARWQL
                     SRKVIQSLAARYEVDSINRIKPGIAEATRAVLRRVPDHVFVRQMDDPDVSLLVGLAKE
                     KGIQVTEAGDTLGQYRAVTIIKKVR"
     gene            complement(3716..4489)
                     /locus_tag="L2X49_004493"
     CDS             complement(3716..4489)
                     /locus_tag="L2X49_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799472.1"
                     /translation="MNKPVVFSDLDDTLFQTRRKMVDELALEPFRTGALDRSLTPRSF
                     MTEEQAMLVDWLLEHADLIPVTARGTEEISRVQIPFRSWAVTTHGAVILRPDGTPDSD
                     WKAHMLECLSSYADRLTSMQHIITELMDARGINAWARLNYEYEGTPVYLVMKHRDSTK
                     LDELNAVGDEIERVFSTEGFYIHRNSNNIAWLPCPVEKGLAVSWLLEKLRQERGVFPV
                     MGLGDSLSDHRFMKLCSWFGLPRQSQFAEAISQRIFGEQ"
     gene            complement(4482..5624)
                     /locus_tag="L2X49_004494"
     CDS             complement(4482..5624)
                     /locus_tag="L2X49_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HBN4799473.1"
                     /translation="MSAIDGNFVYQKTLSCGRIEVSSEVTDYPLTDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVSPQVMRNVYTQLAEKFPGTLDGPVVFIGMAETAVGLGAGVFDE
                     VRKHQPDCVYLTSTRHPVDAELLCEFKEEHSHATDHLIYYPVCAGMRQTVAEAQTLVL
                     VDDEATTGKTFLNLLAALRASGALRKVRRVIAVTLTDWSGEALYQNCPLPLHTVSLIS
                     GSWRWTPEPDAPLPVMPQVNVTSRGNIPITGKQSWGRLGMQIPASDLGLQVQVSPGEN
                     ILVLGTGEFVWEPFLLAERLETAGAQVVFSSTTRSPISTGYAIQSAISFSDNYGLGIP
                     NYVYNVAHQQFDRILICCETPVASVDPRLLEALSAVAPTLEVITYE"
     gene            complement(5636..6694)
                     /locus_tag="L2X49_004495"
     CDS             complement(5636..6694)
                     /locus_tag="L2X49_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBN4799474.1"
                     /translation="MTKKIWFMEGLSSQRDIVLGVKSFAEKENQNIVFFSSHRNERNE
                     ILSVSDFSMLEPKSEEERLSFIHSITSAHGINAIHTGRNCKWFESHRENIQRSGVHLT
                     TGSTGTHWLELADEKVTFSEFMEKNGLPVVPSVRVKNVDELRALLSDPPFPSDCLCIK
                     PVTGIYGMGFWRFDDNVSPVAFLNNPDNRVIHPSQYLAAHARMDCFTPQVLMPYLPGP
                     EYSVDMVVSQGTVLAAIGRRKVGVLQYLENEGEAIELAIDCARLMNADGMVNVQTRHN
                     AEGKPLLLEINMRPSGGIGYTRHSGVNLPGLYAFHTLGLMDDEQVRQSAKTDFSPAVV
                     RSVTDVIPYPASLTNRID"
BASE COUNT         1627 a         1811 c         1642 g         1646 t
ORIGIN      
        1 ttataatttc atctatcttc atttcgacca gtttttcaac tgcttcgctt cttgatgttc
       61 cgctcagctc tgccagttta tctattttcc ggagaatacg gcgtctaatc gacagggaaa
      121 cggaagtttt tatatcctta tccagtatca cccgcgcccc ctggtcatgt gtttttaaat
      181 cagtattaag gtccagcgcc tcactcattc tcttgagtgt tttcttatcc agtaatccgg
      241 agtttaccat ctgataggag tggccagcct tactgtattt aatttcagca gcgtaggttt
      301 ccctcagctc tttaagcgcc cggcttagag tcggctcaga acaggccagc gtcgcggtga
      361 tggattgcgc cgatacaggc ttccctgagc ccagtatgtt agccagggtg tatactcttt
      421 cctgtctggt gtttaattgc atattcagtc ggtacctgcc atatgggtca gtggttctat
      481 aggctgaaaa atgtaagtcg cactcatttt cctgatcaga cctcgatcac ctaatgcttc
      541 aatggggtca aggccacgca gattcccgaa gttgattagc tcgatttcag agcggcatac
      601 ccgccaggaa ataatgtact cgtcttcaag atatccagtg cgtttactta cccgctgagt
      661 gaagcctgaa atgacgacct gttcaaacgg aagcgtctga aaaacgattg ctgctaatct
      721 cagcaggcat ccgtgaacgt gtttagcgta actttcccgg accgctttct gcgtcatggc
      781 tttttcattg atatcagttc cccgggcatt cacgctatac actttatcag gcatatcctc
      841 aatctcaggt aaatcaacat caagccagac tgttgattcc tcaggtcgta cctcaaacga
      901 caccagtgtt tctcgcggcc attctgtttg cgagaaaact tcctcaagac aatgttcaaa
      961 gaagcgggcg tcagatcgga attgctcttt cgctaaagct acagactccg tgcgattttt
     1021 ttcatgctcc tggtactgat gctgccattc tgcgtactgc tcagcccagg ctatacgctg
     1081 tttctcataa cgctgcttca gcaactcatt ttcacgttca atatcagcga ctgtcacctg
     1141 ccaccgacga agattttcgt tctgtcgctc ctgacgggct gcatcagatt caaaccatcc
     1201 cccaaggaag ccgcgaccgg catgctcatc aggttctggt ggaagtggta tatattcagg
     1261 tttgtttggt cgtaccggcg caggtagact gaaactgccc tgacggagcg ccaggtactg
     1321 tgtctcgagc atggcccagg tattccctcc ctttggggaa ggggagagtt catggatatt
     1381 ggttatcact gttacggtgg acatcagttt ttccaccacc agctccagtt cagacttcaa
     1441 accgggatct gcctgatgcc tggtattcac tctttcccgt gctgttctgt caatccgggt
     1501 gcggtaactc agtcccgttc cgggaagtcc caggttggca aatgttccat tctttcctac
     1561 tgacagcgag gctccacggg gaccaatact caggctgggg gccccgttac tcaggttaac
     1621 cctgaccccc gggataatgc tgatcgattt acgaaatctg aatcccatat atgcttcctc
     1681 agtgctgctt agaaactgga ataagcctga ttccttccgg attttgcccg gtagtcagac
     1741 cgtatacctg tccccgtccc aggatcccct gagcccacct gcgatgggta gccggttcac
     1801 acattgctcc ctgagattta aagactgcct gggtggaatt gagaattcgt aacgcatctg
     1861 aatgctcacc aggagacacc tttagcgcct cctctatcac actaatctga tcaggatgaa
     1921 tggccgtttt acccaccagc ccatgggcga tatccatcga gagctcccgt cccagaactc
     1981 cgtgatcgtc gatgtgttcg catactggtg cggtaagtgc aaaatcacga ggtgcgaata
     2041 ctgacacaag catttttatg acataaccca taggagtgtc ataaagggtc agatgccgtg
     2101 gacgtcgcag agataccacg ttcatgagat cgtttccgcc gatccgcaaa gcgattatac
     2161 ggtcacggca gtcatgactc gaaagttcat ttgccagcgc ctgcatttta ataacatcat
     2221 agacttcttc agtttcaagt gttggcatca tcatcaatga tgttccggcc atgatgtccc
     2281 accaaaccgg gagtgtggaa cgggtgaatt tgggcagtac aaaaccatca actgcactca
     2341 ggtccaaatt agccgttagc catctcccca tatcagggtg gcgtggacgg ataaatacca
     2401 gtgggtactt ctcatcatcg ttgacggctc ttgcgtgtct caatcgctcc aaaaacagtg
     2461 aaagattctg aatcgcaaga gggatgtcat gctcgctgac cgcatcttcc aggcagacaa
     2521 ccagagagcg tagccccggt attttccctt ccagaacgac ctcagcgata tccttgcgtg
     2581 tggcaggcat atacagcgtg gcaccgagtt cccatgggtt gggttgtttt ttcatcagcg
     2641 tacctttttg ataatggtaa ccgcacgata ctgaccgagc gtatccccgg cttccgtcac
     2701 ctggatacct ttctcttttg ccagcccaac cagaagagaa acatccgggt catccatctg
     2761 gcgaacaaaa acatgatcag gtacacgacg cagaaccgcc cgcgtcgctt cagcgatacc
     2821 cggcttaatg cggttaatgc tgtccacctc ataacgcgca gcaaggctct ggataacttt
     2881 tctgctcagc tgccagcgtg cggcattttt ccctgcgtcc cagccagaag cgggaagggg
     2941 tttaccgtca agctgttcac ggaaatgtgc taccgtatcc accagcatct ggctacattc
     3001 aaaagactga aggtgatcgc aaaccacaca gccatgcagg ccttcttcgg cccagacaga
     3061 gcgcgaaatc agacccgaga caggcgcgcc catgataccg aacgggataa gccagtcatc
     3121 gtcactggca gcaagccagg cacatccgca ggggtcagcc agcacaacca gtcgaggctg
     3181 ctccggataa ccgggacggt ccttcagtgt gcgggataat tcgccggtaa tagctccttt
     3241 tcccgtccag ccgtcgacaa acactatgcc accagtacca tggcgtgatt cgataaaatc
     3301 aagcgccgca gcatcaatac ccctgtcgcg gataatgctg atcccgtagt ggaaagactg
     3361 ttttcccatc gcacgcagag cctgatgcag catgaccccc agtggaacac cagctctgac
     3421 caggctgacc aggacaatgg gttcatcgcc agcacgacgg gccagttcac gagcaagata
     3481 agatacctcc cgtgccagac gtgcagctcc cgagtcaagc gcacgggtga aaagatccag
     3541 gtgccagcgg gtcggctctg gttcctggct taacatgtcg gaataatgca tttttcctga
     3601 ctgaatcagt tcttccttca ggtcaaccgg tgtcatctcc atgacaatgg gtttcagcaa
     3661 aaagtgaaca tcgtccggtt tataggaacc agaaaacggg gcaaaatgcg acatcttatt
     3721 gctctccaaa aatacgctgt gatatggcct cggcaaactg actctggcgt ggaagaccaa
     3781 accagctgca gagtttcatg aaccgatggt cgctcaggct gtcacccagc cccatcaccg
     3841 ggaaaacccc gcgctcctga cgcagtttct ccagcaacca tgaaacggcc agcccttttt
     3901 ctaccgggca gggtaaccag gcaatgttgt tgctgttacg gtggatgtaa aacccttcgg
     3961 tcgaaaaaac gcgctcaatc tcatccccga cagcgttcag ttcatcaagc ttcgtgctgt
     4021 cgcggtgctt catgaccaga tacaccggcg taccctcata ttcgtagttg aggcgtgccc
     4081 aggcattaat gcctcgcgca tccatgagct cggtgatgat atgttgcatt gatgttaagc
     4141 ggtcggcgta ggaggacagg cactccagca tatgggcttt ccagtcgctg tctggcgtac
     4201 catctggacg aaggatcacc gcgccatgag tggtcacggc ccaggaacgg aaggggatct
     4261 gtacccgact gatttcttcc gtacctctgg ctgtcaccgg aatgaggtcc gcatgctcca
     4321 gcaaccagtc caccagcatg gcctgctctt cggtcataaa gctgcgaggc gtgaggctgc
     4381 gatccagtgc gccggtccgg aacggttcaa gggcaagttc atcaaccatt tttcggcggg
     4441 tctggaacag ggtgtcatct aggtcgctga atactacggg cttattcata ggtaatcacc
     4501 tctaaggtgg gagcgacagc ggaaagcgcc tcaagcagcc gggggtcgac gctggcaact
     4561 ggcgtttcgc aacaaatcag gatccggtca aactgctgat gagcaacgtt atagacataa
     4621 ttcggaatac ccagtccgta gttatcgctg aaagaaatgg cggactggat agcataacca
     4681 gtggaaatcg gtgagcgggt cgtcgaactg aacacgacct gagcacctgc cgtctcaagt
     4741 cgttcagcca gcaggaacgg ctcccagaca aattcaccgg tgcccagcac cagaatgttt
     4801 tcaccgggcg aaacctgaac ctgaagaccc aggtcactcg ccggtatctg catcccgagt
     4861 cgcccccagg attgtttgcc ggtgatcggg atgttccctc tggaggtcac atttacctgg
     4921 ggcatgacag gcagcggggc atctggttct ggagtccagc gccagctgcc tgagatgagc
     4981 gatactgtat gcaggggcag ggggcagttc tgatagaggg cttctccgct ccagtcagtc
     5041 agcgtgacag caataacccg tcgtaccttc cggagtgcac cggatgcacg taatgcggcg
     5101 agaagattga ggaacgtttt tcccgtcgtg gcttcatcat ccacgagcac taatgtttga
     5161 gcctccgcta cggtttgccg catcccggca caaactggat agtagatcag gtggtctgtg
     5221 gcatgactgt gctcttcttt aaactcgcac agcagttcag catcaaccgg atgccgtgtt
     5281 gatgtcagat agacacagtc aggttgatgc ttacgcacct catcaaaaac tcccgcccct
     5341 aggccgacag ccgtctcagc catcccgata aataccaccg gcccatccag agtcccggga
     5401 aacttctccg ccagctgtgt ataaacattt cgcatgacct gaggagagac aggaatatgt
     5461 cgacccagca ctttactgac aaacagaaat gcgcgttttg gattacgccg ttcggcgata
     5521 tcaaacagat cagtaagagg ataatcggtg acctcgctgc ttacctcaat tcgcccacag
     5581 gataatgtct tttgataaac gaaattccca tcaattgctg acatatctgt ctccattaat
     5641 cgattctgtt ggtcaatgat gccggatagg gaataacatc ggtgacagag cggacgactg
     5701 caggtgaaaa atcagttttg gctgactgcc ggacctgttc atcatccatc agtccaagtg
     5761 tatggaaggc atagagaccc ggcagattca cgccactgtg ccgcgtataa ccgatacctc
     5821 ctgaaggccg catgttaatt tccagcaata gcggttttcc ttcggcatta tggcgtgtct
     5881 gtacgttgac catgccatct gcgttcatta ggcgggcgca gtcgatggca agttcaatcg
     5941 cttccccttc attctccaga tactgaagca caccaacctt acgccgccca atagcggcca
     6001 ggaccgtccc ttgacttacc accatatcca cggagtattc tggcccgggt aaatagggca
     6061 tcagtacttg tggagtaaag cagtccatcc gggcatgcgc agccagatac tgtgatgggt
     6121 ggataacacg gttatcaggg ttgttgagaa aagctacagg agagacattg tcatcaaaac
     6181 gccagaatcc catgccataa atgcctgtga caggtttaat acagaggcaa tcagaaggga
     6241 aagggggatc actcagtagc gcgcgaagct cgtcaacatt ttttacccga accgacggga
     6301 caaccggaag tccatttttt tccataaact cggaaaaagt aactttttcg tcggctaatt
     6361 cgagccagtg ggtacccgtt gacccggtag tcaggtggac accagaacgt tgaatgtttt
     6421 cccggtggct ttcaaaccat ttacagttgc gaccagtatg tatcgcatta ataccatgag
     6481 cactcgtaat tgaatggata aatgaaaggc gttcttcttc actttttggt tcgagcattg
     6541 aaaaatcgga gactgacaag atctcatttc tttcattgcg atgagacgaa aaaaaaacaa
     6601 tattctgatt ttccttttcc gcaaatgatt ttaccccaag aacaatgtct cgctgggaag
     6661 atagtccttc cataaaccaa attttcttag tcatgtttta taattatcgc ctgatgagag
     6721 gatggt
//
LOCUS       DAFNBJ010000221         6702 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000221 DAFNBJ010000000
VERSION     DAFNBJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.221"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            209..1741
                     /locus_tag="L2X49_004496"
     CDS             209..1741
                     /locus_tag="L2X49_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBN4799475.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(1793..2512)
                     /gene="fadR"
                     /locus_tag="L2X49_004497"
     CDS             complement(1793..2512)
                     /gene="fadR"
                     /locus_tag="L2X49_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBN4799476.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            2733..4274
                     /gene="nhaB"
                     /locus_tag="L2X49_004498"
     CDS             2733..4274
                     /gene="nhaB"
                     /locus_tag="L2X49_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBN4799477.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4420..4950
                     /gene="dsbB"
                     /locus_tag="L2X49_004499"
     CDS             4420..4950
                     /gene="dsbB"
                     /locus_tag="L2X49_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBN4799478.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(4996..6264)
                     /gene="umuC"
                     /locus_tag="L2X49_004500"
     CDS             complement(4996..6264)
                     /gene="umuC"
                     /locus_tag="L2X49_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBN4799479.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(6264..6683)
                     /locus_tag="L2X49_004501"
     CDS             complement(6264..6683)
                     /locus_tag="L2X49_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBN4799480.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         1595 a         1709 c         1662 g         1736 t
ORIGIN      
        1 accctgcggc aatatggcgt ctttagtatc tcctgctgaa cgcccttttt atccagcata
       61 aaattccgtt cagaagcgga ttagtggcac tctgataata agaaatcgca aagaaaaaaa
      121 ttctccggcg acgcggtttt taacaggtgt tctatgcttg aaatgaggtg ctccacacag
      181 agagcgccga caacaatgag gtgcgcgtat ggcgacgatc gattctatga ataaggacac
      241 cacacgtttg agcgatggac ccgactggac gttcgacctg ctggatgttt atctggcaga
      301 gatagaccgg gtggcgaaac tctaccggct ggatacctac ccgcaccaga ttgaagtgat
      361 aacctcagaa cagatgatgg atgcctactc cagcgtcggc atgccaatta actatccgca
      421 ctggtcattc ggtaaaaagt ttatcgagac tgaacggctg tataagcacg gtcagcaagg
      481 actggcctat gaaatcgtca ttaactctaa cccgtgtatc gcttacctga tggaagagaa
      541 caccattacc atgcaggcgc tggtgatggc acacgcctgc tatgggcata actctttctt
      601 taaaaacaat tacttattcc gcagttggac cgacgccagt tcgattgtcg attatctgat
      661 ttttgcccgt aaatatatta ccgagtgcga agaacgttat ggcgttgatg aagtagaacg
      721 gcttctggac tcgtgccacg cgctgatgaa ctacggcgtg gaccggtaca aacgcccgca
      781 aaaaatctcg ctgcaagaag agaaagcccg gcagaaaagt cgcgaagagt atctacaaag
      841 tcaggtcaat atgctctggc gtaccctgcc gaagcgcgag gaagagaaaa cggttgctga
      901 agcgcgccgc tatccgtccg aaccacaaga aaacctgctc tattttatgg agaaaaatgc
      961 gccactgctg gaatcatggc agcgtgaaat cctgcgtatt gtgcgtaagg tgagccagta
     1021 tttttatccg caaaaacaga ctcaggtgat gaacgaaggc tgggcgacct tctggcacta
     1081 taccatcctt aaccatctgt atgatgaagg gaaagtaacg gaacgtttta tgctggagtt
     1141 tttgcacagc cacaccaatg tggtcttcca gcccccctat aacagcccgt ggtacagcgg
     1201 catcaacccg tatgccctcg ggttcgccat gttccaggat attaaacgga tttgtcagtc
     1261 gccaacggaa gaagacaaat actggttccc ggatatcgcc ggttccgact ggctggaaac
     1321 gctgcatttc gcgatgcgtg atttcaaaga tgagagtttt atcagccagt ttctgtcacc
     1381 gaaagtgatg cgtgatttcc gcttcttcac cgtgctggat gatgatcggc ataattatct
     1441 ggagatttcc gctattcata atgaagaagg ttatcgggag atccgtaacc ggttatcgtc
     1501 gcaatataac ttaagtaatc tggagccgaa tattcagatc tggaacgtgg atttgcgcgg
     1561 cgaccgttcg ctgacgctgc gttacattcc acataatcgc gcaccgctgg atcgggggcg
     1621 caaagaagtg ctgaagcatg tgcatcgcct gtggggattt gatgtgatgc tcgaacagca
     1681 aaacgaagat ggcagcgtcg agttgctgga acgttgcccg ccaagaatgg gaaatctgta
     1741 aaaacaacaa aaaacccctc gtttgagggg tttgctcttt aaacggaagg gattatcgcc
     1801 cctgaatggc taaatcaccc ggcagatttt tctgcatccg gtgccaaatc tcgccactct
     1861 catgcccata gcgacgcact gtttcgtaca cctgatcgtg cgcgccttca ctgcacaacg
     1921 ccgacagttt gtggtagaag cccagcgcca gactgcgcgc ttccggattg gcgaaatagt
     1981 gacgaccaat acgcgtatac agccctttca tcccgttaag aatcagaccg taaatcgggt
     2041 tgccggaagc aaacgccagg ccgcggaata tgttgtaatc cagctcggca aaggcatcgg
     2101 cgtgatcggc cacttcatta gcggtagcca gcacttcctg cgctttatcg ggatgctgac
     2161 gaaacgcggt gcgaataaaa atagtggaaa tattggtacg caccgacagc aaattatcaa
     2221 taagctgcgg cacactttcg tgatccagtc gcgccagtgt ttcaaggata tttaaaccgg
     2281 aagtttccca gaaattattc accttcgtcg gcttgccatg ttgaatggtc aaccagccat
     2341 ctcgtgccag acgctgtaac acttcacgta acgtagtacg cgttacgcca attaattctg
     2401 aaagttcacg ttctgcgggc aaaatagtcc cgggagggaa gcggttattc cagatacttt
     2461 caataatgta ctcttccgcg aaacccgccg ggctttgcgc cttaatgacc atagtgagat
     2521 ttccataaca cagcaaaaca aagttggact catcatacca gagggctaac tacgctgata
     2581 gcagacaaaa agaagaaaaa gggatcaagg cttcattttc cgcatctctt tccccttgac
     2641 tatcaagact tgcctcactc ctgcctgacc gttaagcttt gcgtttaaac ataaaaacat
     2701 cattgattat ttgtaaggta agggaaacca tcatggagat ctcctggggc cgcgcgctat
     2761 ggcgcaattt tttgggccag tcccccgact ggtacaaact cgccctcatc attttcttaa
     2821 tcgtaaaccc gttaattttc ctcatcagcc ctttcgtcgc gggctggttg ctggtagcgg
     2881 aatttatttt cactctggcg atggccctga aatgctaccc gctgctcccc ggtggtctgt
     2941 tggctatcga agcggtattc atcggcatga ccagcgcgga acacgtccgt gaagaggtgg
     3001 cggcaaatct tgaagtcttg ctgttactga tgtttatggt ggcgggtatc tattttatga
     3061 aacagctgtt gctgttcata tttacccgtt tgctgttaag cattcgctcc aaaatgctgc
     3121 tgtcgctctc tttttgcgtg gcggctgcgt tcctctccgc gttcctcgat gccttaaccg
     3181 tcgtggcggt ggtgatcagc gttgcagtcg gtttttatgg tatttatcat cgcgtagcct
     3241 cttcccgtac cgaagacacc gacctgcaag acgatagtca tatcgacaag cattacaaag
     3301 tggttctgga acagttccgt ggctttctgc gtagcctgat gatgcatgcc ggtgtcggca
     3361 ctgcattagg cggcgtgatg accatggtgg gcgaaccaca gaacctgatc atcgctaaag
     3421 cggctggctg gcattttggc gatttcttcc tgcgcatgtc gccggtgacc gttccggttc
     3481 tgatttgtgg cctgttaacc tgcctgctgg tagagaagct gcgttggttt ggctacggtg
     3541 aaacgctgcc ggagaaagtc cgcgaagtgt tgcaacagtt tgacgatcaa agccgccacc
     3601 agcgtacccg tcaggataaa atccgtctga ttgtccaggc gattattggc gtctggctgg
     3661 tgactgcgct ggcgttgcat ctggcggaag ttggcttgat tggtttgtca gtcattattc
     3721 tggcgacatc attgaccggt gtcaccgatg aacatgctat cggtaaagcc ttcaccgaat
     3781 ctctgccatt caccgcactg ttgacggtgt ttttctcggt agtcgcggtg attatcgacc
     3841 aacaactgtt ttcgccaatt attcagtttg tgttgcaggc atcggaacat gctcagctgt
     3901 cgctgttcta tattttcaac ggtctgctgt catccatttc ggataacgtc ttcgtgggga
     3961 cgatttatat caacgaagcg aaagcggcaa tggaaagtgg cgctatcacg ttgaagcaat
     4021 acgagctgct ggcggtcgcc attaataccg gtaccaatct gccctccgtc gctacgccga
     4081 acggtcaggc tgcgttcctg ttcctgctga cctctgcact cgcgccattg attcgcctct
     4141 cttatggccg catggtgtgg atggccctgc cttacaccct cgtactgaca ctcgtcggct
     4201 tgctctgcgt cgagtttacg cttgcccctg taaccgaatg gtttatgcaa atgggctgga
     4261 tagcaacgct ttgataacaa cttaccgggc atataaatgc ccggtttgcc ttttcgcccg
     4321 ataattgtcc aattgcttcc tatttttgct gcctcctggt ggcgcagcga atgaattggt
     4381 ttaaactgcg cactctatgc atattgcagg gaaatgatta tgttgcgatt tttgaaccaa
     4441 tgttcacaag gccggggcgc gtggctgttg atggcgttta ctgctctggc actggaactg
     4501 acggcgctgt ggttccagca tgtgatgtta ctgaaacctt gcgtgctctg tatttatgaa
     4561 cgttgtgcgt tattcggcgt tctgggtgct gcgctgattg gcgcgatcgc cccgaaaact
     4621 ccgctgcgtt atgtagcgat ggttatctgg ttgtatagtg cgttccgcgg tgtgcagtta
     4681 acttacgagc acaccatgct tcagctctat ccttcgccgt ttgccacctg tgattttatg
     4741 gttcgtttcc cggaatggct gccgctggat aaatgggtgc cgcaagtgtt tgtcgcctct
     4801 ggcgattgtg ccgagcgtca gtgggatttt ttaggtatgg aaatgccgca gtggctgctc
     4861 ggtattttta tcgcttacct gattgtcgcg gtgctggtgg tgatttccca gccgtttaaa
     4921 gcgaaaaaac gtgacctgtt cggtcgctaa tcccttcagc gctcctgcgg gagcgctttt
     4981 ttcctgccgc tatatttatt tgacccgcag taaatcagaa cttcgcgttg tataacgtgg
     5041 tgaaagcatg gcacgcttca tctgccattg ttgctggatc ccctgcccgg caaaatagag
     5101 tgttcctctg ccctctttag cattcagtgt atccattacc gtcatcaatt gctcactccc
     5161 ggggcgcggt gcgttgtcat cgaataaatt gagctgcgcg actccctgac tgaagaaatc
     5221 ccccagcatc acgcccgctt tttggtagcg atggcccgct tgccagatgg catccagaga
     5281 tcgcgtagca gcgttaatga tatccctgct gtcctgagtg ggcgtcagca gttttaccga
     5341 cgcgctattg ccgtaataag gttcattgag cgcaaatggt gacgtcttaa taaatgtgga
     5401 gataaaccga caatattgat gctcgctgcg aagtttttcc gccgcccggg cagcgtaact
     5461 acaaatggcc tgccgcatcg atggataatc cgtgatgcgt tcaccaaacg agcgggaaca
     5521 gataatttcc tgcttcgtcg gtgcaaactc ttccagttgc aaacagggtt cgccgcgcag
     5581 ttcacgcacc gttctttcga gcacgacatt aaaatgttta cggataaacc ggatatctgt
     5641 atccgccaaa tcgagaacgg ttttgatccc catcgcgtcc agttttttgc tgatccgccg
     5701 tccaatcccc cagacgtcat ccacggggag agcagacatt aatttacgct ggcgttccag
     5761 atttgataaa tccaccaccc cacccgtctg ccgctgccat ttttttgccg catgattggc
     5821 aagcttagcc aacgttttgg tctgggcgat ccccacacca acagtaagat gggtacgttg
     5881 tagcaccgtt gcgcgaattt ctctgccaaa atcagtcaga tcgcgacaat tacgcacacc
     5941 tgtcagatcg cagaatgcct catcaatact gtaaatctcg acgcggggcg atagttcttc
     6001 cagcgtcgac atcacccgat tgctcatgtc tgcgtaaagc tcataattgc tgctaaagca
     6061 aaccacgcca cagcgacgaa acagatcttt ttgtttgaac cagggatcgc ccattttaac
     6121 acccagcgcc tttgcctcag cgtttcgggc gataacgcaa ccgtcattat tcgatagcac
     6181 aaccaccggt ttaccccata aatcagggcg aaacaccgtc tcacagctgg cataaaacgc
     6241 gtttacatca cagagagcaa acatcagcgc atcgccttaa cgacgtggat caccacacca
     6301 aagacatcca gcgtatcttc gctactgatg gtaatgggcg agtacgcact gttcatggga
     6361 ataagctgta ccgtcgggcg tagttgcaat tttttcaccg taaactcgcc gtcaacagca
     6421 gcgatgacaa tatcaccatg gctggcggta atagcgctat cgacaatcag taaatcaccg
     6481 tcactaattc caccatcaat catagaatca ccacttgctt tgacgaagta agtcgcgctg
     6541 ggatgctgga tcaacagttg attcagatcg atgcgctgtt caacgtaatc tgctgccggt
     6601 gaaggaaagc cacactgaac aagatcgcta aatagcggaa aagtcacaat ttcgcggaga
     6661 tccgcaggct tgataaacaa cataataatc tgcctgaagt ta
//
LOCUS       DAFNBJ010000222         6558 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000222 DAFNBJ010000000
VERSION     DAFNBJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.222"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            21..584
                     /gene="srlA"
                     /locus_tag="L2X49_004502"
                     /gene_synonym="gutA"
     CDS             21..584
                     /gene="srlA"
                     /locus_tag="L2X49_004502"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBN4799481.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAVFEKKMGIQLEQKVHLAGATS"
     gene            581..1540
                     /gene="srlE"
                     /locus_tag="L2X49_004503"
                     /gene_synonym="gutE"
     CDS             581..1540
                     /gene="srlE"
                     /locus_tag="L2X49_004503"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBN4799482.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1551..1922
                     /gene="srlB"
                     /locus_tag="L2X49_004504"
                     /gene_synonym="gutB"
     CDS             1551..1922
                     /gene="srlB"
                     /locus_tag="L2X49_004504"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBN4799483.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1926..2705
                     /gene="srlD"
                     /locus_tag="L2X49_004505"
     CDS             1926..2705
                     /gene="srlD"
                     /locus_tag="L2X49_004505"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBN4799484.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2810..3169
                     /gene="gutM"
                     /locus_tag="L2X49_004506"
     CDS             2810..3169
                     /gene="gutM"
                     /locus_tag="L2X49_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBN4799485.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3236..4009
                     /gene="srlR"
                     /locus_tag="L2X49_004507"
                     /gene_synonym="gutR"
     CDS             3236..4009
                     /gene="srlR"
                     /locus_tag="L2X49_004507"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBN4799486.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4002..4967
                     /gene="gutQ"
                     /locus_tag="L2X49_004508"
     CDS             4002..4967
                     /gene="gutQ"
                     /locus_tag="L2X49_004508"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBN4799487.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4964..6478)
                     /gene="norR"
                     /locus_tag="L2X49_004509"
     CDS             complement(4964..6478)
                     /gene="norR"
                     /locus_tag="L2X49_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBN4799488.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
BASE COUNT         1508 a         1799 c         1725 g         1526 t
ORIGIN      
        1 aaatcctgaa ggagagaaca atgatagaaa ccattactca tggtgcagag tggtttatcg
       61 ggctgttcca aaagggcgga gaggtgttta ccgggatggt gaccggcatt cttccgctgt
      121 tgattagcct gctggttatc atgaacgcac tgattaattt tatcggtcag catcgtattg
      181 aacgttttgc tcaacgttgc gccggtaacc ctgtttcccg ttacctactg ttaccgtgca
      241 ttggcacgtt tgtcttttgc aatccgatga ccttaagcct gggtcgcttt atgccggaaa
      301 agtacaaacc cagctactac gcggcggcct cttatagctg ccactcaatg aatggcctct
      361 tcccccatat caaccctggc gaactgtttg tttatcttgg cattgccagc ggtctgacaa
      421 cgctgaacct gccacttggc ccactggcgg tgagttatct gctggttggt ctggtcacca
      481 atttcttccg cggctgggtg accgatctga ccaccgccgt ttttgagaaa aagatgggca
      541 ttcaacttga acaaaaagtt cacctggcag gagcaacatc atgacgcgta ttcggatcga
      601 aaaaggaacg ggtggctggg gcggcccgct tgagctggaa gccacgccgg gcaaaaaaat
      661 cgtctatatc accgccggta cccggcctgc gattgttgac aaactggcac aacttactgg
      721 ctggcaggct attgacggat ttaaagaagg tgaacccgcg gaggcggaaa ttggtgtcgc
      781 ggtaatcgac tgtggcggca cattacgctg cggcatctat ccgaaacggc gtattcccac
      841 cattaatatc cactcgacgg gcaagtccgg tccactggcg cagtacattg tggaagatat
      901 ttatgtctct ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga caccacaacc
      961 ctcttccgtg ggccgtgact atgacaccag caagaaaatc accgaacaaa gcgatggttt
     1021 actggcgaag gtgggaatgg ggatgggttc tgccgttgcc gtgttgtttc aatctggtcg
     1081 tgacaccatc gacactgtat taaaaaccat tctgccgttt atggcattcg tctcggcgct
     1141 cattggcatc attatggctt ctggccttgg tgactggatt gcccacggtc ttgctccgct
     1201 ggcgagccat ccactgggtc tggtcatgct ggcgctcatc tgctccttcc cactgctttc
     1261 acctttcctc ggcccaggcg cagttatcgc acaggttatc ggcgtattga ttggcgtgca
     1321 gattggtctc ggcaatattc cgccgcatct ggctttacct gcactgtttg ccatcaacgc
     1381 gcaggcggcc tgcgacttca tcccggtcgg tttgtcgctg gcggaagctc gtcaggacac
     1441 ggttcgcgtc ggtgtccctt ctgtactggt gagccgcttt ttaaccggcg cgccgactgt
     1501 actgatcgcc tggtttgtct ccggttttat ctatcaatag aggctgaaac atgaccgtta
     1561 tttatcagac caccatcacc cgtatcggcg cgagtgccac tgacgccctc agcgaccaga
     1621 tgctcatcac ctttcgtgaa ggcgcgccag cggacctcga agagtattgc ttcattcatt
     1681 gccacggcga gttgaaaggt gcactccatc ccggtttgca attttcactc gggcagcatc
     1741 gctatccggt gaccgctgtt ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg
     1801 tcaccctgcg cttcgatggt ttaaacgaag cggaatttcc gggcactgtc catgtggcag
     1861 gccctgtacc cgacgatatc gcgccgggat cggttttgaa gtttgaatct gttaaggagt
     1921 aaaaaatgaa tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc gcgttcctgt
     1981 gccacggtct ggctgccgag gggtatcgcg tcgcggttgt cgatattcag agcgacaaag
     2041 ccgcaaatgt ggcacaagaa attaacgccg aatatggtga aggtatggcg tacggttttg
     2101 gtgctgacgc caccagcgag caaagcgttc tggcgctctc tcgtggggta gatgaaatct
     2161 ttggtcgcgt ggatttgctg gtctatagcg ccggaatagc aaaagcagcc tttatcagcg
     2221 acttccagct cggcgatttt gaccgttcgc tacaagtgaa tctggtgggt tatttcctgt
     2281 gtgcccgtga attttcgcgt ttgatgatcc gcgacgggat tcagggtcgc attattcaga
     2341 tcaactcgaa atccggcaaa gtgggtagca aacacaactc tggctacagc gcagcgaaat
     2401 ttggtggcgt cgggctgact caatcactgg cgctggatct ggcggagtac ggcattacgg
     2461 tgcattcact gatgctcggt aacctgctga aatcgccgat gttccagtca ctgttgccac
     2521 aatacgcgac caagctgggt atcaaaccgg atcaagtcga gcagtattac atcgacaaag
     2581 taccgctcaa acgcggctgc gattatcaag atgtgctgaa tatgctgctg ttctacgcca
     2641 gtcctaaggc gtcgtactgc accggacagt cgatcaatgt caccggcggt caggtgatgt
     2701 tctgatcaac agcggagatc cgttaaggat ctccgtgaga ctatagaatg cctgatgcgc
     2761 tacgctcatc aggcatacag gacttccgcc actacattaa ggaaaagtta tggtatccgc
     2821 actcatcacc gtcgccgtta tcgcctggtg tgcgcaactg gccttaggcg gctggcaaat
     2881 ttctcgtttt aaccgtgcct tcgacacact atgccagcaa gggcgggttg gcgtgggccg
     2941 ttccagcggg cgctttaaac cgcgggtcgt ggtcgccatc gccctggacg atcagcagcg
     3001 catcgtcgac accttgttta tgaaaggact gaccgtcttc gcccgaccgc aaaaaattcc
     3061 cgcaattacc ggtatgcatg cgggtgattt acagcccgat gtgatctttc cccatgatcc
     3121 actatcacag aatgctctat cattggcgct taaactgaaa cgtggataat ttcgttgtga
     3181 atgttacttg cttgcgaagt tatcattttg aaacctaaat caggtaatca cgcccatgaa
     3241 acctcgtcag cgtcaggccg ccattctgga gtatctgcaa aagcagggta aatgctcggt
     3301 tgaagaattg gcgcaatact ttgacaccac aggcacaacc attcgcaaag atctggtcat
     3361 tctggaacat gccggaaccg tcattcgtac ttatggcgga gtggtgttga ataaagagga
     3421 atccgatccg cctatcgatc ataaaacgct catcaacacc cacaagaaag agctgattgc
     3481 agaagctgcc gttagtttta ttcatgatgg cgactcaata attcttgatg ctggcagtac
     3541 cgttttgcag atggtccccc tgctctcgcg ctttaataac atcacggtga tgaccaacag
     3601 tctgcatatc gtcaatgcgc tatctgaact ggataacgaa caaaccatcc tgatgccagg
     3661 cggaacattt cgcaaaaaat cggcctcatt tcacgggcaa ctggcagaga atgccttcga
     3721 gcatttcacc ttcgacaaat tattcatggg tactgacggc atcgatctca atgcgggcgt
     3781 aaccaccttc aacgaggtct ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt
     3841 gattttaatg gcggactcat caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct
     3901 tgaaagcgtc gataagctga ttaccgacgc aggtatcgat ccggcgtttc gtcaggcgct
     3961 ggaagagaaa gggatcgatg tgatcataac cggagagagc aatgagtgaa gcactactga
     4021 acgcgggacg tcagacgtta atgctggagt tgcaggaagc aagccgttta ccggaacgtc
     4081 tgggcgatga ttttgttcgc gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg
     4141 tttcgggaat tggcaaatcg ggccacattg gtaagaaaat cgccgcaacg cttgccagta
     4201 ccggcacacc ggcttttttt gtccatccgg cagaagcact gcacggcgat ctggggatga
     4261 ttgaaagccg cgatgtgatg ctgtttatct cttactccgg tggcgcgaag gaactggatc
     4321 tgattattcc gcgtctggaa gataaatcta tcgcgctgct ggcgatgacc ggcaaaccga
     4381 cgtcaccgct gggcctggcg gcaaaagcgg tgctggatat ctccgtagaa cgcgaagcct
     4441 gcccgatgca ccttgcgccg acctccagca ccgtcaatac cctgatgatg ggtgacgcgc
     4501 tggcgatggc ggtcatgcag gcgcgcggat ttaatgaaga agattttgcc cgctcccacc
     4561 cagccggggc actgggcgct cgcttgctga ataaagtgca tcatctgatg cgccgtgatg
     4621 acgctatccc gcaggtagcg ttaaccgcca gcgtgatgga tgcgatgctg gaactcagtc
     4681 gcaccggttt ggggctggtg gcagtatgtg acgctcaaca acaggtacaa ggcgtcttta
     4741 ccgacggcga tttacgtcgc tggctggttg gcggcggcgc actcaccacg ccagttaatg
     4801 aagcgatgac gacaggcggc accacattac aggcacaaag tcgcgccatc gacgccaaag
     4861 agatcctgat gaagcgcaaa atcactgccg caccggtggt ggatgaaaac ggcaaactca
     4921 ccggcgcaat taacctgcag gatttctatc aggccgggat tatttaatcc ttcaatccca
     4981 gacgtttcgc cagccgatgc aggttggcga cgtcggtttc cagcatccgc gcgcaggcag
     5041 cccagttgtg atgattttgt gccagtgcct ggcgaatggt ttcacgctgg aacgcttctg
     5101 tcgcttcacg caggttttgc ttaacaacgg gcaccgccgc cgcttctggc ggcggcaacg
     5161 tcacctcagg aaaagcaaaa tgttgcgcct caagaatcac ttcatcgccg ttgcgggtgg
     5221 ctctcgacag cactaccgcc cgatgaatag catgttccag ttcgcgcacg ttccccggaa
     5281 aacgatagtg ttgcagtaaa tttcgcgctc cggcacttaa taccacgcgg gagagcccca
     5341 gccgcaaacg acactgctcg cagaaatacc ccgccagcag aatgacatca tcgccccgct
     5401 cacgcagcgg cggcaccgaa agtggaaaca cgctcaggcg atgaaacaag tcagcgcgaa
     5461 atcgccctgc cagcacctct tcgcgtaagt cgcggttagt cgccgccagc acgcgcacat
     5521 cgacccgcaa actgcggtca tcgccaacgc gctgaatatc gccatactgc aacaccctca
     5581 gcagcttggc ctgcaatgcc aacgacaact cgccgatctc atccagaaac agagtgccgt
     5641 tatccgccat ttcaaacttc ccgctgcggt tactgatagc gccagtaaac gctcctttca
     5701 catgcccgaa caactcactt tccgccacac tttccggcag tgcagcacag ttgagataga
     5761 ccagcggatt caccgcccgt ggcgaggctt catgaatcgc tttcgccacc agctccttac
     5821 cggttcccgt ctcaccgctg atcaggacgt tgagatcgga cgccgccaca atctcaatct
     5881 cttttttcag ttgcgtcatg ccaggggaca agccaatcat ctgcgtctgt ttcaccgctt
     5941 caaacggcgt ggcatcgcct ggcatcatat tctggctttc cagttgttca atcagcaacg
     6001 cattgcttaa cgctcccgcc gccagcgcag caatcagccg tagctcttcg tcgctgaaaa
     6061 catcgaactg atcgggctgc atcccgtcga gcgtcagtgc gccgatcagg ttttgcccgg
     6121 caaacaatgg cagaccaacg caggcgtgaa ccttcagact ctcctgccca ggaatcaaac
     6181 cgtcataggg atcgggcaat tcgctgtctg cgggaaagcg caccacatcc ccggcgcggg
     6241 caatcgcttc cagccgtgga tgcccttcca gcgcaaagcg tctaccgagt acatcctttg
     6301 ccagaccgtc gatggcaagc ggaataaact gccgcgaatc gtaacgtagc aacgcagacg
     6361 catcgcactc cagcacctga cgtagcgtgg tgatcaggcg ctgaaaacga tcctgatgac
     6421 caatcccacg ctgcaattcg atggcgatat tcgccagcac atcaacggaa aaactcatct
     6481 ttgcctcact gtcaatttga ctatagatat tgtcatatcg accatttgat tgatagtcat
     6541 tttgactact cattaatg
//
LOCUS       DAFNBJ010000223         6440 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000223 DAFNBJ010000000
VERSION     DAFNBJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.223"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..391
                     /locus_tag="L2X49_004510"
     CDS             <1..391
                     /locus_tag="L2X49_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN4799489.1"
                     /translation="LDIDSFKSINDKWGHHMGDQVIVMVTRIIKKSIRKEDVLGRLGG
                     EEFGIIIKGNTQKLLLSIAERIRKNIEEQCSEKLLSHGPEKITVSIGCFTSKENNLSP
                     SEMLVNADKALYQAKRTGKNKVIIHSK"
     gene            complement(465..734)
                     /locus_tag="L2X49_004511"
                     /pseudo
     CDS             complement(465..734)
                     /locus_tag="L2X49_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            791..1459
                     /locus_tag="L2X49_004512"
     CDS             791..1459
                     /locus_tag="L2X49_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBN4799490.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1514..1645)
                     /locus_tag="L2X49_004513"
                     /pseudo
     CDS             complement(1514..1645)
                     /locus_tag="L2X49_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(1697..2404)
                     /locus_tag="L2X49_004514"
                     /pseudo
     CDS             complement(1697..2404)
                     /locus_tag="L2X49_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            complement(2350..2910)
                     /locus_tag="L2X49_004515"
     CDS             complement(2350..2910)
                     /locus_tag="L2X49_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBN4799491.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIKRRRSLSTRMRLTQQLI"
     gene            3299..3532
                     /locus_tag="L2X49_004516"
     CDS             3299..3532
                     /locus_tag="L2X49_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HBN4799492.1"
                     /translation="MSTKNRTRRTTIRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQIRPD"
     gene            3589..3999
                     /locus_tag="L2X49_004517"
     CDS             3589..3999
                     /locus_tag="L2X49_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HBN4799493.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(4351..4503)
                     /locus_tag="L2X49_004518"
     CDS             complement(4351..4503)
                     /locus_tag="L2X49_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799494.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(4491..4958)
                     /locus_tag="L2X49_004519"
     CDS             complement(4491..4958)
                     /locus_tag="L2X49_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBN4799495.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDNKASEL
                     EKANATITDMQQRQRDADALDDKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(4955..5452)
                     /gene="rrrD"
                     /locus_tag="L2X49_004520"
     CDS             complement(4955..5452)
                     /gene="rrrD"
                     /locus_tag="L2X49_004520"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBN4799496.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSALLRKINQGDIKGACDQLRRWAYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5452..5667)
                     /gene="essD"
                     /locus_tag="L2X49_004521"
     CDS             complement(5452..5667)
                     /gene="essD"
                     /locus_tag="L2X49_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBN4799497.1"
                     /translation="MKSMDKLTTGIAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(5855..>6440)
                     /locus_tag="L2X49_004522"
     CDS             complement(5855..>6440)
                     /locus_tag="L2X49_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN4799498.1"
                     /translation="IVDVEEKTVNDFFKSNTLSPFSVRRFYPAYLMVECEDFSLLKNL
                     IACLNCDGRTVDFVRNQISLACLAILSSEKIVQSFLFGCLNSLGSKVKAIIHTDISAA
                     WRLCDISSRLYLSESLLKRKLKHEGLSFSKLILEERMVMAERLLSYNLYSVGKVAEIC
                     GYENTSYFVSVFRRYFGVPPHQYSSRFFLEKDMM"
BASE COUNT         1874 a         1508 c         1308 g         1750 t
ORIGIN      
        1 gttagatatt gacagtttca aaagcatcaa tgacaaatgg gggcatcata tgggtgatca
       61 agtcatagta atggttacca gaataataaa aaaatccatc aggaaagagg atgtattagg
      121 gcgcttaggc ggtgaggagt tcggtattat cattaaaggt aatactcaaa agctcttgct
      181 atcaattgca gagcgaatca gaaaaaacat tgaagagcaa tgctcggaaa aattattatc
      241 gcatggacct gagaaaataa ctgtcagtat tggttgcttt acttcaaaag agaataatct
      301 cagcccatct gaaatgttag tcaatgccga taaagcgtta tatcaagcca aaagaaccgg
      361 aaaaaacaag gtgataattc actcaaaata aacacctttt taaaatacag ccccaataaa
      421 ctgcagaata ttatcccata taatatcctg cagttcgtaa tgcactattc gataatgggt
      481 actgttggcc attcaatatc cggtgcagtt gttgtattaa cacggttcag caacacccga
      541 tacttcttcc aggcttccag caacgagttt tcttcctccg ttgcgatctc cagatctaca
      601 gcatcctgaa gtggcgcaat atgctcactg aattcctgga tgtagaacta tgtggtgacg
      661 gtcttccagc cattcggctc ctgctgtatc gaagcatacc aggctatttc aatatcgcta
      721 tgctgcggca gcatttaacc ccttgtaatt catcaccata attgatttaa ttcacaaaca
      781 aaactataac atggtgaaat taatgaaaaa aaacacagat gatggggcta aaatttacac
      841 accacttacc ctaaagcttt atgactggtg ggttttggga gtatcaaatc ggcttgcatg
      901 gggatgtcct acaaaggaac accttcttcc acactttctg gaacatttag gtaacaacca
      961 tctggatatt ggtgttggaa ctgggtttta ccttactcac gtacctgaga gtagtctgat
     1021 atctttaatg gatttgaacg aagctagcct gaacgcggca tctacaaggg ctggggaatc
     1081 aaaaattaaa cataaaatta gccatgatgt ttttgaacct tatcccgcgg cgttacatgg
     1141 tcaatttgat tccatttcca tgttttacct tcttcactgc ctgcctggaa atatatctac
     1201 aaaaagctgt gtaatacgca atgcggcgca ggccttaact gacgatggaa ctctatacgg
     1261 agccacaatt cttggcgatg gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat
     1321 ttacaatcag aaaggcatct tttcaaacac aaaagattcc gaagaaggct taacacatat
     1381 actctcagag catttcgaga atgttaaaac caaggttcaa ggtactgtag taatgttttc
     1441 cgcttcaggg aaaaaatagc atccaaccgc agctcgctct tgcttaagac gtgctgcggc
     1501 ataatcccaa tgattactcc ctgacagggt tcgtaggcca ctcaatatca ggtgcagttg
     1561 atgtatcaac acggttcagc aacacccgat acttcttcca gacttccagc aacaaggttt
     1621 cttcctccgt tgcgatttcc agctcaacaa cagtctgaac gtaccaggaa cagcctcctt
     1681 cagggcttga aggatatcaa tgttcgcttc ctgttaactg cccgacaagt gcaaccagtt
     1741 cgcttacctg attttccaga gtggtgatcc gggtgcctgc tgttgccaga ttttcacgta
     1801 acgttgtatt ttcctcttcc agcgcggtaa cgcgatcatc tgtttcacgg gcgacctgaa
     1861 caagtaaccc cgtcacggcg gagtagtcaa cattaagata gcgagtttct tcgcgtagct
     1921 cgttgccgtc aacggtcgga ccttgcaact cttcaccata atgagtaaac gatcccacag
     1981 cttcaggtat cgcctccatt acttcctgtg caataacgcc agcataaggc attccgtttt
     2041 ccttgagcgt gtaggtgtac ccgttcattt tacggattgc tttcgtcgcg tcgctgataa
     2101 cgagaatatc gtctttaagg tcgcggtctg atgactgatt cagcgttgtg caattaatag
     2161 cgccatttac atcaaacaac tggcctgctg acgttttttg cgcataaaac agatacgcag
     2221 cagacgttcc aacctcaaaa acgttttgtc gagtactgga accccacacc ctgacagaaa
     2281 acggtagttc tgcattacct gagttctgta aaacaaaacg attgccagtc cctgattgtt
     2341 ttgtaagggt taaatcaact gttgagttaa cctcatcctt gttgatagtg agcgcctgcg
     2401 ctttatcagt tcatccagcg cggctgcttt gttcatggct ttgatgatat cccgtttcag
     2461 gaaatcaaca tgtcggtttt ccagttccgg aaaacgccgc tgcaccgaca gggggatccc
     2521 gtcgagaata ctggcaattt cacctgcgat ccgcgacagc acgaaagtac agaatgcggt
     2581 ttccaccact tcagcggagt ctctggcatt tttcagctcc tgtgcgtcgg cctgcgcacg
     2641 cgtaagtcga tggcgttcgt actcaatagt ccctggctgg agatctgtct cgctggcctg
     2701 ccgcagttct tcaacttccc ggcgcagctt ttcgttctca atttcagcat ccctttcggc
     2761 ataccatttt atgacggcgg cagaatcata aagcacctca ttacccttcc caccaccccg
     2821 cagaacgggc attccctgct cctgccagtt ctgaatggta cggatactcg cgccgaaaat
     2881 gtcagccagc tgctttttgt tgacttccat tgttcattcc acggacaaaa acagagaaag
     2941 gaaacgacag aggccaaaaa gcccgttttc agcacctgtc gtttcctttc ttttcagggg
     3001 gtgttttaaa taaaaacatt aagttacgac gaagaagaac ggaaatgcct taaaccggaa
     3061 aattttcata aatagcgaaa acccgagagg tcgccgcccc gtaacctgtc ggatcgccgg
     3121 aaaggacccg caaaatgata ataattatca tctgcatgtc acaacgtgca tctacgccat
     3181 caaaccacgt caaataatca attatgacgc aggtatcata ttaattgatc tgcatcaact
     3241 taacgtaaaa acaacttcag acaatacaaa tcagcgacac taaatacggg acaacctcat
     3301 gtcaacgaag aacagaaccc gcagaacaac aatccgcaac atccgctttc ctaaccaaat
     3361 gattgaacaa attaacatcg ctcttgatca aaaagggtcc gggaatttct cagcctgggt
     3421 cattgaagcc tgccgccgga gactgtgctc agaaaaaaga gtttctcctg aagcaaacaa
     3481 agaaaagagt gacattactg aattgctcag aaaacagatc agaccagatt gaagcaattt
     3541 agataatcgt gcagactacg cccctcatat cacatggaag gtactacaat ggctcaggtt
     3601 gccattttta aacaaatatt cgataaagtg cgaaataatt taaactatca ctggttttat
     3661 tctgaactaa aacgtcacaa tgtctcacat tacatttact atttagctac agagaatatt
     3721 catcttgttc ttgaaaacga taatacggtt ttaataaaag gacagggtaa ggttgtaaat
     3781 gtaagatttt caaaaaataa atgccttata gaagccacct taaaaggatt caaatcagga
     3841 gagttatcat tttacgaata caggaaaaat cttgctacag caggggtttt cagatggatt
     3901 acaaatatcc acgaaaacaa aaggtattac tatacctttg ataattcatt actctttact
     3961 gagaacattc agaacactac acaaatattt ccgcactaaa tcataacgtc cggtttcttc
     4021 cgtgccagaa ccggactcgc tggcatgatg aaatatgtgt acccggtaac cccggtgtgc
     4081 atcgtttttg attattcccg cacactcgcg cagaaggagt tccccgtcgg gctacggtct
     4141 ctgttaatac gggaatacgg cgacgataca gcgcatgatg tgtcaggctt gaataccttt
     4201 atcctttaaa agggatatca gttaagttat cccgtgtagg gtataaacca ttatcaaagc
     4261 cactctgtag gaagtggctt ttgtaatggc aataaaaagc cccgcgaatg cgaggctaaa
     4321 tcctggtatt tgtaatgact ggttcttatc tcaacgcagc cccttaccgc gcgcaaaatg
     4381 ctcaatatca agcatcagca atgagatgtt taatctggat tcactccaga agtgagcacc
     4441 accctgtcta cagagccaga tgtgaaggat gatgagtaaa attatcgcta tcatcgaagg
     4501 cattgcgtcc tgatgtattc ctgaagcgtt ctcagtgctg tttggtcgcg gataattccg
     4561 tcccggatac cgagaacgtt tcgtccagca actggagaga gttcgacggt ggcatcattg
     4621 cccatgccgg aggcgctgga ggtttcggct gaggatggca cagggcattt tcctttgacg
     4681 agcacccgac caccattatc aagcttgcgc cgaagagcat cattttcagc tttcgcatca
     4741 gctaactcct tcgtgtattt atcatcgagt gcatcagcat cacgctggcg ctgctgcatg
     4801 tcagtaatgg tggcgttcgc cttctccagt tcactggcct tgttatcgcg ctgttctttg
     4861 taggcgattg cattatcacg gtaatgatta acagcccatg acaggcagac gatgatgcag
     4921 ataaccagag cggagataat cgcggttacc ctgctcattg ttgcccccac aaacagacct
     4981 cacgctcaat ctcacgacga gtcatcaggc ctttccattg cttaccgcca gcgtatgccc
     5041 agcgacgtag ctggtcacat gcgcccttga tatcgccctg gtttattttg cgaagaagag
     5101 cggatgttct gaaattgcct gcgcccacgt tatagacgaa cgagtaaaga gcgccgcgcg
     5161 ttgtttccgg tatatcgact ttgatgtacg ggttaatttg tctggctacc gtggcaaggt
     5221 ctttattcag gagggctttg cattctgctt cggtatacgt tttaccggga atgatgtctt
     5281 ttcctgtatg cccgtgacat acagtccata cgccaacgat atctttgtat ggtatgtagc
     5341 tgacaccttc cagaccatcg ttaccacttg ggccagtgat taacactgat gctatagcaa
     5401 ttgctccgcc accaatagca gcagcaacgg cttttcgtaa tgatggaggc attattcacc
     5461 tctcgcagcc ttgcgcttat cttctttaat cttgaaataa aggtttgtca ggtacgtcag
     5521 caggccaaat accaagctac ccagcacacc tattgctgcc cactgtgagg gcgtgacttt
     5581 atcgagcagc tgtaaaaacc agtagccagc actgcctgcg gaggtgccgt aggcaatgcc
     5641 cgttgttaac ttatccatgg atttcatagc ctcacctccg caaataacgg atggtgtaca
     5701 cggttcggaa cgaagaggaa aggtatagaa gttacattag cgtaaggctt gaacatctat
     5761 tcaaaaagaa aaacgccgcg attattctgg cgtagctgaa agcatcatac aattatcaaa
     5821 tacgaaaatt acaaaatcat taaaacgcat cacgttacat catgtctttt tctaaaaaaa
     5881 atcttgatga atattgatgg ggaggaacac caaaatacct tctgaaaaca cttacaaaat
     5941 atgacgtgtt ttcataaccg catatctcag caacttttcc aacagaatat aaattgtagc
     6001 ttaataacct ttccgccatc accattcgct cttcaagaat taacttacta aatgataagc
     6061 cttcgtgctt taattttctt tttaacagac tttcactcag atacagtctt gaagatatat
     6121 cacaaagtct ccatgctgca gatatatccg tgtgaataat agccttaact ttacttccta
     6181 aactattaag acatccaaat aaaaaacttt gcactatttt ctctgaagat aagatagcaa
     6241 gacatgcaag tgatatttga tttctaacaa aatccacagt tctgccatca caattcaagc
     6301 atgcaatcaa gttctttaac aatgaaaaat cttcacattc caccatcaag tatgccggat
     6361 aaaaccttct tacagaaaaa ggtgagagtg tgttgctttt aaagaaatca ttaactgttt
     6421 tttcttcaac atctacgatc
//
LOCUS       DAFNBJ010000224         6391 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000224 DAFNBJ010000000
VERSION     DAFNBJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.224"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(120..542)
                     /locus_tag="L2X49_004523"
     CDS             complement(120..542)
                     /locus_tag="L2X49_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBN4799499.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEVKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKEPSAVDPDLIWSLPDG
                     EIRRYDRRHNMICTECRKSEVMQRILSFYQGDVRYLLK"
     gene            complement(583..1548)
                     /locus_tag="L2X49_004524"
     CDS             complement(583..1548)
                     /locus_tag="L2X49_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799500.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGLKCAAPSGQNDTMEEVKMKRSIGSKRLNVIGSKWPDDLTENTT
                     EITTENKKTSRPEASQPDPQTVEQDFLTRHPDAVVFSAKKRQWGSQEDLACAQWIWGR
                     IVSLYEQAASDDGEISRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSSVQRCVNHISEPDTEIPPGFRG"
     gene            complement(1529..1771)
                     /locus_tag="L2X49_004525"
     CDS             complement(1529..1771)
                     /locus_tag="L2X49_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799501.1"
                     /translation="MRISDFRLYREISDGKSITYMIAGLNKEYGDVVESGLLFADPAV
                     VDRETDELIEKAIAFKLAYRQQYQQKAGWNYESSFC"
     gene            1855..2043
                     /gene="dicB"
                     /locus_tag="L2X49_004526"
                     /gene_synonym="ftsT"
     CDS             1855..2043
                     /gene="dicB"
                     /locus_tag="L2X49_004526"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HBN4799502.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            2040..2231
                     /gene="ydfD"
                     /locus_tag="L2X49_004527"
     CDS             2040..2231
                     /gene="ydfD"
                     /locus_tag="L2X49_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HBN4799503.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVINQDNIEIPAGL"
     gene            2325..4796
                     /locus_tag="L2X49_004528"
     CDS             2325..4796
                     /locus_tag="L2X49_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBN4799504.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPITSIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKVIDFDARTAIQFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            4869..5120
                     /locus_tag="L2X49_004529"
     CDS             4869..5120
                     /locus_tag="L2X49_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBN4799505.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            5155..>6391
                     /locus_tag="L2X49_004530"
     CDS             5155..>6391
                     /locus_tag="L2X49_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBN4799506.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLS"
BASE COUNT         1879 a         1485 c         1462 g         1565 t
ORIGIN      
        1 tgattgaaat aacccccatt ttgcaaagtc cgctcttggc acaaagcgga caaccacgct
       61 agctctaccc tgtgccacaa aatgtcaatt tgcatctgaa ctaatgcact ttaatctcgt
      121 cacttcaata aataccggac atccccctga taaaacgaca atatgcgctg cataacttcg
      181 cttttacgac actcagtaca aatcatatta tgacgcctgt cgtaacgacg tatttctccg
      241 tcaggtaatg accagataag gtccggatca accgcagatg gttccttcag ctttgccctt
      301 gagagctttt tacgggcatt ttgccagtcc ttacgcgcct gttcagacgg gaataacccg
      361 taaccagagt tgtatacatc gccgctggca accagctctc ttgcgagaac gctcatcaga
      421 tatcttgtcg cacctgtctt gacttccagt tgccgtaacg tctcacgccc actccggcgt
      481 acgagttcaa caacctgccc tttaattttt tcccgctctt cttgtgtaaa aacttttgcc
      541 acaagccctc ctgaaaatta cctcatgacc agaaattaac acttaccccc tgaagcccgg
      601 cggaatttcg gtatccggct cagaaatatg attcacacaa cgctgtacag acgaacgccc
      661 caggcggata accagttcat cccatttttc gcgaagcttt gacggactca tgatattttt
      721 tacccagaat ggatcccgct gcacccgacc aaacatttca caaatttgtc tgtgagttct
      781 gccatccagc atccgcattg tgcgcacgtc gttggcccat gcggtccagt tgggttcttt
      841 cggtcgcgaa atctcgccat catcgctggc ggcctgctcg taaagactca cgattcgccc
      901 ccagatccac tgcgcacacg ccaaatcttc ctggctgccc cactggcgtt ttttcgcact
      961 gaacacaacc gcgtcagggt gtcgggttaa aaaatcctgt tcaaccgtct gcgggtccgg
     1021 ttgcgaagct tccggacgag aagtcttttt attctctgta gtaatctctg ttgtattctc
     1081 tgtaagatca tcaggccatt ttgacccgat gacattgagt cgttttgaac caatggagcg
     1141 tttcattttg acctcttcca tcgtgtcatt ttgacctgat ggagcggcgc attttaaacc
     1201 gatggattcg ctcaatttgc caccatctaa aagctcgctc ccgtagttga tcgtgtagaa
     1261 attggtcata tcgcgctttg atttattgag cttttcacaa cgcaaaagcc ccagcgtttt
     1321 cagacttgca aacgcgcgct ttaacgttga ctctgaccag aatgggaact gttccagcca
     1381 ttgttccgtt gtgttataaa tccagcgaac accatcacat tccatgccgg agttggtatc
     1441 tctcaaccag tagtgcagtt gttgcaaaac aatggcttcg tttaagccaa ttttcattgc
     1501 cagctgcgtg tttataacca gtgggcgttc agcaaaaaga agactcataa ttccatccag
     1561 ctttttgttg gtattgctgt cgatacgcaa gcttgaaagc aattgctttt tctataagtt
     1621 cgtcagtttc acgatctaca acggcaggat ctgcaaaaag cagtccggat tccaccacat
     1681 cgccatattc tttatttaac ccggcgatca tgtacgtaat actttttcca tcactgatct
     1741 cacgatacaa cctgaaatca ctaatccgga tatccgacgt taattgttaa ctggttaacg
     1801 tcacctggag gcaccaggca ctgcatcaca aaattcattg ttgaggacgc gataatgaaa
     1861 acgttattac caaacgttaa tacgtctgaa ggttgttttg aaattggtgt cactatcagt
     1921 aaccctgtat ttactgaaga tgccattaac aagagaaaac aagaacggga gctattaaat
     1981 aaaatatgca ttgtttcaat gctggctcgt ttacgtctga tgccaaaagg atgtgcacaa
     2041 tgaattcagc atttgcgctt gttctgacag tttttcttgt ttccggagtg ccagttgata
     2101 ttgcagtcag tgttcacagg acaatgcagg agtgtatgac tgcagcaacc gaacagaaaa
     2161 ttcccggtaa ctgttacccg gtcgataaag ttattaacca ggataatatc gaaatcccgg
     2221 caggtcttta aaacagttcc gtaataaata tccggtttca ttcttatatg ccagcaatgg
     2281 cagggatttg ttcatcctta aatctgtcat gaggttaaaa caaaatgagt aaagtcttta
     2341 tttgcgccgc tattcctgac gaactggcaa caagggaaga aggcgctgtg gctgtagcca
     2401 cagccattga agctggcgac gaacgccgtg ctcgagcaaa atttcactgg caattcctgg
     2461 aacattatcc ggctgctcag gactgcgctt ataaatttat tgtctgcgag gataaacctg
     2521 gcataccccg ccctgccctc gattcatggg atgctgaata tatgcaggaa aaccgctggg
     2581 atgaggagtc tgcttctttt gtcccggttg agactgaatc cgatccgatg aacgtcactt
     2641 ttgacaagct ggcccctgaa gtacagaacg ctgtcatggt taagttcgac acatgtgaaa
     2701 acatcaccgt tgatatggtt attagcgcac aggaattgtt gcaggaagac atggcaacat
     2761 tcgacggaca tatcgttgaa gcgttgatga aaatgccaga agttaacgcc atgtatccgg
     2821 agcttaagtt gcacgccatt gggtgggtta agcataaatg tattcctggt gctaaatggc
     2881 ccgaaattca ggcagagatg cgcatctgga aaaaacgtcg cgaaggtgaa cgcaaggaaa
     2941 ccggaaaata cacgtctgtt gttgatctcg cacgcgccag agccaatcaa cagtacactg
     3001 aaaattcaac aggaaaaatc agcccggtca ttgctgccat tcatcgcgaa tacaagcaga
     3061 catggaaaac actggatgac gaactggcct acgctctctg gcctggtgat gtggatgccg
     3121 gaaacattga cggcagcatc catcgctggg caaaaaaaga agttatcgac aacgaccgcg
     3181 aagactggaa gcgtatctcg gcatcaatgc gcaaacagcc tgatgccctt cgctacgacc
     3241 gccaaactat ttttggcctt gtccgtgagc gtccgatcga cattcacaaa gatcccgtag
     3301 cactgaacaa atatatctgc gaatacctga cgacaaaggg cgtgtttgag aatgaagaaa
     3361 cagacctggg cactgttgat gttctccagt catcagaaac acaaactgat gcagtggaaa
     3421 ctgaggtatc tgatatccca aaaaatgaaa ccgcgccgga agctgaacca tctgtagagc
     3481 gtgaggggcc gttctatttc ctcttcgcag ataaggacgg agaaaaatac ggtcgcgcaa
     3541 acaaactttc tggtctggat aaggcactgg ctgctggcgc cactgaaatc acaaaagaag
     3601 aatattttgc ccgaaaaaat ggcacataca cgggcttacc gcaaaatgta gataccgctg
     3661 aagattcaga acaaccagag ccgataaaag ttaccgctga cgaagtaaac aaaattatgc
     3721 aggcagccaa tatcagccag cctgacgctg ataagttgct tgctgcatca cgtggtgaat
     3781 ttgttgaagg gattagtgac ccgaatgatc cgaaatgggt taaggggatc cagacccgcg
     3841 attctgtgaa ccagaaccag catgaatcgg aacggaacta ccaaaaagcg gaacaaaaca
     3901 gtccaaatgc gttacaaaac gagccagaaa cgaaacagcc tgaaccagta gcgcaacagg
     3961 aagtggaaaa agtctgcacc gcctgcggtc agaccggcgg cggcaactgc cctgattgtg
     4021 gcgcggtgat gggcgacgca acataccaag aaacattcga tgaagagtat caggttgaag
     4081 ttcaggaaga tgatccggag gaaatggaag gcgctgaaca tccacacaag gagaacactg
     4141 gcggcaatca gcatcacaat agcgataatg aaactggcga gacggcagat cacccaatta
     4201 aggtgaacgg tcatcacgaa atcacatcca ccagcaggac gtgtgaccat ctaatgatcg
     4261 accttgaaac catgggaaaa aatcctgatg ccccgatcac ctcaataggt gcaatatttt
     4321 tcgatccgca aaccggagat atgggaccgg aatttagtaa gactatcgat ctggaaactg
     4381 ctggcggagt cattgatcgg gacaccatta aatggtggct taagcaatca cgcgaagcgc
     4441 aatctgccat tatgaccgat gaaatcccgt tagatgatgc actgttacaa ttgcgggaat
     4501 ttatcgacga aaactccggt gaattttttg ttcaggtttg ggggaatgga gccaacttcg
     4561 acaacacgat tttgcgccgt tcatacgaac ggcaggggat cccctgcccg tggcgttact
     4621 acaacgatcg cgatgtacgc acaatcgttg agctggggaa agtcatagac ttcgatgcca
     4681 gaacggctat tcaattcgaa ggtgagcgcc ataatgcact tgatgacgcc cgttaccagg
     4741 caaaatacgt ttcagttatc tggcaaaaac tgatcccgag tcaggctgat tcttaatgtt
     4801 caactgtcgc cggttgtgac tggtattctg caaccggcgc tcgtctaatg taagagataa
     4861 agaaatcgat gagcgaagta atcatgattg tctctcccgg caaatgggta tccgaagagc
     4921 agttaattgc gctgaaagga ataaaaaaag gtacgttaaa aaaggcccgg gaaaaatcgt
     4981 ttatggaagg aagggaatat aagcatgtcg ctcatgacgg tatgccatgg gataacagtc
     5041 catgctttta caacctggaa gaaattgatc gctggattga gcgccaggca tcagcgagac
     5101 caagacgtca tcttacttga ctaaaagcca cactaactaa tgagagaagt tgaaatgaaa
     5161 tatccgacag gcgtggaaaa ccatggaggg aaattacgta tctggtttgt ttataaaggc
     5221 gtaagagtca gggaaaacct gggggttcct gacacagcaa aaaacaggcg cgttgcaggt
     5281 gaactacgct cctctgtttg ttacgcaata aaaactggtg ttttcgacta tgcaaaacag
     5341 tttccctcct cacgcaatct ggaaaaattt ggtgaggccc gacaagattt aaccataaaa
     5401 gaactggctg aaaaatttct ggcactgaaa gaaactgaag tcgccaaaac atcactcaac
     5461 acataccgtg ccgtcatcaa aaatatcctg agcataatcg gtgaaaaaaa tcttgcctca
     5521 tcgattaata aagaaaaatt actggaggtt cgtaaagagt tactgactgg ataccagatc
     5581 cccaaaagta actatattgt tacacaacca gggagatcgg ctgtaactgt aaataattac
     5641 atgacaaatc ttaacgccgt gttccagttt ggtgttgata acggttacct ggcagataat
     5701 ccgtttaagg ggatctcgcc attaaaggaa tcaagaacca ttccggatcc tctttcgcgg
     5761 gaagaattta tccgtcttat cgatgcgtgc agaaatcagc aagcaaaaaa tttatggtgt
     5821 gtttctgttt atactggcgt tcgccctggt gagctgtgtg cacttggatg ggaggacata
     5881 gatctgaaaa atggaacaat gatgatcagg agaaatttag caaaagaccg tttcacggta
     5941 ccaaaaacac aggcgggaac caatcgggtc attcatctta ttaagccagc aatcgacgct
     6001 ctccggagtc agatgacatt aacgagactg agcaaagagc atatcattga tgttcacctc
     6061 agagagtatg gcagaacaga aaaacaaaaa tgcacctttg tttttcaacc tgaagtgtca
     6121 gcgagagtaa aaaattatgg tgaccatttt accgttgact caataaggca gatgtgggac
     6181 gcagcgataa aacgtgccgg actccgccat cgaaaatcat atcagtcgag acatacttat
     6241 gcctgctggt cgctgacagc tggtgctaac ccggcattta tagcaaacca gatgggccat
     6301 gcagatgcgc aaatggtatt tcaggtatac ggaaaatgga tgtctgaaaa caataatgca
     6361 caggtagctt tgttaaatac acagttaagc g
//
LOCUS       DAFNBJ010000225         6366 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000225 DAFNBJ010000000
VERSION     DAFNBJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.225"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..813
                     /locus_tag="L2X49_004531"
     CDS             1..813
                     /locus_tag="L2X49_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4799507.1"
                     /translation="MIGTLVNISKIIIQKRELIWQMSKRDVMGRYKGSFLGLAWSLFN
                     PILMLTVYTFVFSVIFKSRWGSSPDAGHADFAIILFTGLIIFNLFAECINRSTSLITS
                     NVNFVKKVVFPIEILPIVNLLAALFHASISLIVLFIAILVFKHQLHFTVLLLPVITIP
                     LMLSILGLSWILASLGVFVRDIPQTISIIVTILMFLSPVFYPLSALPVVFQKIVMLNP
                     LAFMIEEARKVVYWGNEPNWTMLAINMLIGLVICAAGFLFFQKTKKGFADVI"
     gene            803..2212
                     /locus_tag="L2X49_004532"
     CDS             803..2212
                     /locus_tag="L2X49_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN4799508.1"
                     /translation="MSSDNIAISVSNISKCYNIYQNPRDRLKQFFLPKVQSMVGKPVQ
                     RYYNEFWALNNINFTINKGESAGIIGRNGSGKSTLLQLITGTLTPTTGTIQTKGRIAA
                     LLELGSGFNPEFTGRENIYLNGAVLGLSRAEIDAKFDAIAGFADIGTHLDQPVKTYSS
                     GMMVRLAFAVQVQLEPDILIVDEALAVGDALFQKRCFQQIEKLTSNGVTLLFVSHDQE
                     SVRTLTNRAILLDRGTQLSTGLSSEVLLEYRRLLHREESKYFSQLAKEVAAHADATRD
                     INQTPTAPAKIEISANAETPAVEETSPSRTDKLSFGEGEAEVENVELFTASGERSNAF
                     NPGDKIRVRVNCKVNKDINHINVGIRIRNKEGVKIYSWGTLNQDMYVRFHQLKDPQFW
                     EREFKAGERFYVDMEFECTLGTNLYEIQAAISYEGTPNYFSQRILHWRDEAAFFHVNV
                     LRDEYFFGGILDLKMTAEW"
     gene            2217..4334
                     /locus_tag="L2X49_004533"
     CDS             2217..4334
                     /locus_tag="L2X49_004533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799509.1"
                     /translation="MNDISITDYLGPGVYLLQNYPKETEGLIAEKGYKVHNCADLAQC
                     KDILNRNKVNFLLTNDKDNNFNEYAKIVRTAARQLVNKIVINIFVEKGNGQSFQDFIN
                     ITDNLGYSIDTVFYLLNPGYDEQFRDDQSLKIVLSYRRQSVVSTDKNILETTIFEKKI
                     VNTLPYIRPGDRVLVIIKNKNSITNIKNIIAEQTKASEVEIYSLDEIKSVQLNGNGYH
                     FLITDKYADDGLNNALKVIISYLVPAGRYVSFHTDKTVVETLSNYNLQPEVYLFYEHG
                     LLKTQIHQGEEITLSPELCVFMKSPLARSELPYQETIYGYSHPPKNLLAFARDYTNPW
                     LIRGIVEFPFRNRSTYHLQQYSHQILEHSAPDSPDYAAALAVLGYQMLSGSDDTADIY
                     AKMLDYCSNVSQMDNPTPHQYRWLISLSTLLGLICNKNNDKANALIHLSRAANSSIDK
                     FSPSIGTKILQSFYLQSVILISLNRISCAEIIVDRGIKRGIQLLYQHPDELVGKISQP
                     FNFVLYIYHDILDWLIKMVNIKNAIPGRKFNIANFDNGNTWSALLHERMNAINNMSQM
                     IDERDRTIHDQKCLIDERDRTIHDQKRLIDERDSTVLTQKNLIDERDLVSAQQNQLIE
                     QNNKTIQQLIQNVTDLNSQVSSKEQKVDELQNQNIKLISLIDEKDLHIAQLSTDLERA
                     NTILRKINSTPVIRHLLRMLNIK"
     gene            4355..5545
                     /locus_tag="L2X49_004534"
     CDS             4355..5545
                     /locus_tag="L2X49_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008926069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HBN4799510.1"
                     /translation="MANIAWFIPQLIEGSGGHRTMLQHAAYLEKMGHTCTIFLEDKSG
                     KTSGAEVIHKLFGLKFKDVIYDWNNAQPCDAAVATIWYSAPFVAALPFDCKRLYFVQD
                     FEAYFNPVGDAYLMAENSYCYGLKPITIGRWLRYKLQNDFAVESYHFEFGANHNIYKR
                     VPTIQKEKAVCFIYQPDKPRRCSRIGIEALGIVKHCMPDVKIYLYGSSINDNLWFEHE
                     NLGLLDLPKCNELYNKCSVGLCISSSNPSRIPFEMMNAGLPVVEVWRENNLYDFSEDA
                     MLLCKQTPESIAAGIMKILNDADLAASMSSAGQKFMNGRTLETETEGFYKAFNCVMNE
                     QAYTAEVISRSYNKPPFEAPLARPDIANTLTFAHQSLLKRIALRLPLFVRTPLSALYF
                     KLKN"
     gene            5551..>6366
                     /locus_tag="L2X49_004535"
     CDS             5551..>6366
                     /locus_tag="L2X49_004535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799511.1"
                     /translation="MNKFKLKTVDVWDTILRRDCHPDFIKRSTAYYFFLKFHSLHNVD
                     SVDQLFTKRVETERLIGKKNKDLGYDDEYLIQDVMLQWVENYISAEQYDTPAIAHELY
                     SWEIEQEKKHIYLDPGIEDFLAQYPADKTIFLSDFYTSSTDLTELLVSAGLDQSVISD
                     GVSSIDERLNKRSGRLFDFIQQKYQLAGVDWIHIGDNEWSDVQMPTSKGIKSIRYLPA
                     QQHQLREQKEFLWNKNEDLTETITNNILNKYAASKDLSVDFQLGLKTTPLIAGF"
BASE COUNT         1994 a         1205 c         1247 g         1920 t
ORIGIN      
        1 atgattggaa ctttagtaaa tatttcgaag ataattatcc agaagcgcga gttaatctgg
       61 caaatgtcta aaagagacgt tatgggacgc tataaaggat cctttcttgg tctggcatgg
      121 tcacttttca accctatatt gatgttaaca gtctacacat ttgtattctc ggttatattc
      181 aaatctcgtt ggggaagcag tccagatgcc gggcatgcag attttgcaat cattttgttt
      241 accggactga ttatttttaa cttatttgca gaatgtatta acaggtcaac cagccttata
      301 acctcaaatg taaacttcgt caaaaaagtt gttttcccta ttgagatatt acctatagta
      361 aatctgctgg ctgctttatt tcatgccagc attagcctca tagtcttatt tattgcaata
      421 ctggtgttta agcatcagct tcattttact gtcctgttac tgccagtcat tactattcca
      481 cttatgctct ctatacttgg attaagctgg atcctggcct cactgggcgt ttttgttcgt
      541 gatataccac aaactattag tatcatcgta acaatattaa tgtttttatc tcccgtattt
      601 tatccgttat cagcattacc ggttgttttc caaaaaattg taatgttgaa tcctctggca
      661 tttatgattg aagaagcaag aaaagttgtt tactggggaa atgagcctaa ctggaccatg
      721 ctcgcaatta atatgcttat tggtttagtt atctgtgctg caggtttttt atttttccaa
      781 aaaacaaaaa aaggatttgc cgatgtcatc tgataacatt gcaattagcg taagtaacat
      841 tagcaaatgc tataacattt accaaaaccc cagagatagg ttaaaacagt ttttcttacc
      901 taaagtccag agtatggttg ggaaacctgt acaacgctac tataacgaat tttgggcgtt
      961 aaataacatc aactttacca tcaataaagg tgagtcagcg ggaattattg gacgtaatgg
     1021 ttctggcaaa agtacattac tacagctaat caccggcacg ctaactccaa caacgggcac
     1081 gatacaaacc aagggaagaa tagctgccct cctggaatta ggctccggtt tcaacccaga
     1141 gtttaccggt agggaaaaca tctatcttaa cggcgccgtt ctgggattat cgcgggcaga
     1201 aatagatgca aaatttgatg ctattgccgg ttttgctgat ataggaaccc atctcgacca
     1261 gccggtaaaa acatactcca gcggaatgat ggtgcgcctt gcatttgccg ttcaggtaca
     1321 attagagcca gatattctca tcgtagatga agcacttgct gtaggcgacg cactttttca
     1381 aaagcgctgt ttccaacaaa ttgagaaatt aaccagcaat ggcgtaactt tactgtttgt
     1441 ttcacacgat caagagtccg tgcgtacctt aacaaacaga gctattcttc tcgatcgtgg
     1501 gacgcaatta agtactggct tatcatcaga agttttactt gaatatcgtc gtttactaca
     1561 ccgggaagaa agtaagtatt tttctcagtt agcaaaggaa gttgctgccc acgcggacgc
     1621 cactcgcgat attaatcaaa cccccaccgc gccggctaag atagaaatta gtgctaatgc
     1681 tgaaacccct gctgtagagg aaacctcgcc ctctcgtaca gataaactat cgttcggtga
     1741 aggtgaagca gaggttgaaa atgttgaatt gtttacggct tctggtgaac gtagcaatgc
     1801 ctttaaccct ggtgataaaa tcagagttcg tgttaattgt aaagtaaata aagatataaa
     1861 ccacattaac gttggcattc gtattcgcaa taaagaaggc gtcaaaattt attcatgggg
     1921 aacgttaaac caggacatgt atgttcgttt ccatcaactt aaagatcccc aattttggga
     1981 acgtgagttt aaagcaggcg agcgttttta tgttgatatg gaatttgaat gtactttagg
     2041 aacaaaccta tatgaaatac aggcggcgat atcctatgag gggacaccta attatttctc
     2101 gcagcgcatt ttacactggc gtgatgaagc cgcgtttttt cacgttaatg tcctgcgtga
     2161 tgaatatttc tttggtggca ttctggatct caagatgact gcagagtggt aagttgatga
     2221 atgatatttc aataactgat tatctcgggc ctggggtgta tttattacaa aattacccaa
     2281 aagaaactga gggcctgatt gcagagaagg gttataaggt gcacaactgc gctgatctgg
     2341 cgcagtgtaa agatattctt aaccgcaata aagttaattt ccttttaacc aatgataaag
     2401 ataataattt taatgaatat gccaagattg tcagaaccgc tgcaagacag cttgtaaata
     2461 aaatcgttat taatatcttt gtagaaaagg gaaacggtca gagtttccag gattttatta
     2521 atataactga caatcttggt tattcgatag acactgtatt ttatttgctc aatcctggct
     2581 atgatgaaca atttcgggat gatcaaagcc taaagatagt tttgagttat agacgtcagt
     2641 cagtagtttc gacagacaaa aatatcttag aaacgactat ttttgaaaaa aaaattgtaa
     2701 atactttacc ctacatacgt cctggggatc gagtacttgt aatcattaag aataaaaatt
     2761 cgataacaaa cattaaaaat attattgcag aacaaacaaa ggcatctgaa gttgaaattt
     2821 actcattgga tgaaattaaa agtgttcagc taaatggcaa tggctaccat tttctcatta
     2881 ctgataaata tgccgatgat ggactaaata atgcactcaa agtcattata tcttacctgg
     2941 tgcccgctgg caggtacgtt agttttcata cagataaaac tgtagttgag acattaagta
     3001 attacaattt acaacctgaa gtttatttat tttatgaaca tggcctttta aagactcaaa
     3061 tacatcaggg agaggaaatt acactctctc ctgaattatg tgtttttatg aaaagccctt
     3121 tagccaggtc agagcttccg tatcaggaaa cgatttatgg ctactcgcat ccaccaaaaa
     3181 acctattagc ttttgccaga gattatacta acccgtggct tattcgcggg atcgtagaat
     3241 tcccattcag gaataggtca acttatcatc tacagcagta tagccatcag attttggagc
     3301 acagtgctcc tgacagcccc gattatgccg ctgcattggc agtattaggt tatcaaatgc
     3361 taagcggcag tgatgataca gctgacattt acgcaaaaat gctagattac tgctcaaatg
     3421 tgtcgcagat ggataatcct acgccacatc aatatcgttg gctcatatct ttatcgacac
     3481 ttttaggtct tatttgtaac aaaaataatg ataaagctaa tgcgttaatc catttatctc
     3541 gtgcagcaaa ctccagcatt gataaattta gcccctctat cggcacgaag attttacaaa
     3601 gcttttatct tcagtccgtt atattaattt ctttaaatag aatctcctgt gccgagataa
     3661 tcgtcgatag agggattaaa agaggtatac agctacttta tcaacatcct gacgaactgg
     3721 tgggtaaaat atctcaacca tttaattttg tgctttatat ttaccatgat attcttgatt
     3781 ggcttataaa gatggtcaac attaaaaacg ccataccagg tagaaaattc aacattgcaa
     3841 attttgacaa cggtaacacc tggtcggctt tgcttcatga acgcatgaat gcaataaata
     3901 atatgtcgca aatgatagat gagcgtgaca gaacaattca tgatcaaaaa tgcttaattg
     3961 atgaacgcga tagaactatt cacgaccaga agcgccttat tgatgagcga gacagcactg
     4021 ttcttacgca aaaaaattta attgatgagc gtgacctggt ctctgctcag caaaatcaat
     4081 taattgagca gaataataaa actattcagc agctaattca aaatgtaact gacttaaata
     4141 gtcaagtttc ctctaaggaa cagaaagttg atgaattaca gaatcaaaac ataaaattaa
     4201 tttcattaat cgatgaaaaa gatctccata ttgcccagct ctctacagat ctagaacgtg
     4261 caaataccat actgcgtaag attaatagca cgcctgtaat aaggcatcta ctacgcatgc
     4321 taaatataaa ataattctat tgtgaaaggt tataatggct aatattgctt ggtttatacc
     4381 tcaactcatt gaagggtctg gcggccaccg cacaatgctg caacatgccg cttatctgga
     4441 aaaaatgggt catacatgta caatttttct tgaagataaa agcggcaaaa caagcggtgc
     4501 agaggtaatt cataaattat ttggtctaaa atttaaagac gtcatttatg actggaataa
     4561 tgcgcaacca tgtgatgctg cagttgcaac aatatggtat tctgcaccat tcgtggccgc
     4621 tctaccattt gattgtaaac gtttatattt cgttcaggat tttgaagctt acttcaaccc
     4681 ggtgggcgat gcttacctga tggctgaaaa ttcatactgt tacggcttaa agccaattac
     4741 gattgggcgc tggttacgat ataaactgca aaatgatttt gctgttgaaa gctatcactt
     4801 cgaatttggg gcgaatcata atatctataa gcgcgttccc accattcaaa aagaaaaagc
     4861 ggtttgcttt atctatcagc cagataaacc gcgacgttgt tcgcgtattg gtattgaggc
     4921 tttaggtata gttaaacatt gcatgccaga tgttaaaatt tatctttatg gttcatcaat
     4981 taatgataat ctctggttcg agcatgaaaa tctaggttta ttagatttgc cgaaatgtaa
     5041 tgagctatat aataaatgct ccgtaggact ctgtattagt tcttcaaatc cctctcgcat
     5101 cccctttgaa atgatgaatg caggccttcc cgttgttgaa gtctggcgag aaaacaatct
     5161 ttatgatttt agcgaagatg ctatgctgtt gtgtaaacag acacctgaaa gcattgccgc
     5221 cggtattatg aaaattctaa acgatgctga tttagccgcg agtatgagct cggctggtca
     5281 aaagtttatg aatgggcgta ccctggaaac ggaaacagaa ggcttttaca aagcgttcaa
     5341 ctgcgtgatg aacgagcagg catacacagc agaagtgatt tcacgatcgt ataataagcc
     5401 cccgtttgaa gcccctcttg ccagacctga tattgccaac acgttaacat tcgcgcatca
     5461 gagtctgtta aagagaattg ctctacgttt accgttattt gtgcgcacac ctttaagcgc
     5521 gctttatttt aagttgaaga attaatccag atgaataaat ttaaactcaa aactgttgat
     5581 gtatgggata caattctgcg tcgtgattgc catccagact tcataaagcg gtcaactgca
     5641 tattattttt tccttaaatt tcattcgcta cacaatgttg attccgtcga ccagctattt
     5701 actaagcgag ttgaaacaga gcgcttaatt ggtaagaaaa acaaagatct tggttatgat
     5761 gatgagtatc tcattcagga tgtaatgctt caatgggtag aaaactatat ctctgcagag
     5821 caatatgata cgccagcgat agcacatgag ctatattcct gggaaattga gcaagagaag
     5881 aagcacatct atctggatcc aggtattgag gactttcttg cgcaatatcc cgctgataag
     5941 acaatctttc tgtccgattt ttatacttca tccactgatt taacagagtt actcgttagc
     6001 gctggattgg atcagtctgt catatcagat ggcgtttctt caattgacga gcgactgaac
     6061 aagagaagtg gacgtctctt cgattttatt caacaaaaat atcagttagc cggcgttgac
     6121 tggatacata ttggcgataa tgaatggtcc gatgtgcaaa tgccgacatc aaaaggtata
     6181 aaaagtattc ggtatctccc agcccaacag catcagctgc gtgagcaaaa agagtttctc
     6241 tggaataaaa atgaagatct cacggaaacg atcaccaata atattcttaa taaatatgca
     6301 gcatctaaag atttatcagt tgattttcag cttggcttga aaacaactcc gttaatcgct
     6361 ggtttt
//
LOCUS       DAFNBJ010000226         6303 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000226 DAFNBJ010000000
VERSION     DAFNBJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.226"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(685..1161)
                     /locus_tag="L2X49_004536"
     CDS             complement(685..1161)
                     /locus_tag="L2X49_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBN4799512.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1220..2509)
                     /gene="bioA"
                     /locus_tag="L2X49_004537"
     CDS             complement(1220..2509)
                     /gene="bioA"
                     /locus_tag="L2X49_004537"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBN4799513.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGKR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2596..3636
                     /gene="bioB"
                     /locus_tag="L2X49_004538"
     CDS             2596..3636
                     /gene="bioB"
                     /locus_tag="L2X49_004538"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBN4799514.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDKEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3633..4787
                     /gene="bioF"
                     /locus_tag="L2X49_004539"
     CDS             3633..4787
                     /gene="bioF"
                     /locus_tag="L2X49_004539"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBN4799515.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4774..5529
                     /gene="bioC"
                     /locus_tag="L2X49_004540"
     CDS             4774..5529
                     /gene="bioC"
                     /locus_tag="L2X49_004540"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBN4799516.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPKGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5522..6199
                     /gene="bioD"
                     /locus_tag="L2X49_004541"
     CDS             5522..6199
                     /gene="bioD"
                     /locus_tag="L2X49_004541"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBN4799517.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1519 a         1637 c         1723 g         1424 t
ORIGIN      
        1 ctggtgatag tatactatta ttctggttaa tcgctgtcaa attgatgatt ttggttagta
       61 ttgcaatagc gaagagtttg atccggtaag actgactagg caaggctata ccaaaaaatc
      121 agtgtaggtt cagaaaacga tgcaccatac aatcatcaat aatttcaaat tgtccattgt
      181 gcataaatca taaagaaaac cgcagcacgt cgtatgcaag aacgtgctgc ggttggctgg
      241 tgaactttcg atagtgcgag tattgaatga tttccagctg ttgccgattt tactatgttt
      301 tcagtagaac acttagacaa aactgaggca cacaaatctt tgcactggat tgcaaggctt
      361 tgtgcttctc tggagtgcga catgtttgat aacaaaaaat tagcgcaaga agacaaaaat
      421 caccttgcgc taatgctctg tctcaggtca ctaatactat ctaagtagtt gattcatagt
      481 gactggatat gttgtgtttt gtagcatcat gtagtctatt ttttagacca atgatattgt
      541 aacacattga tattaatgtt ttttaatgtt tcgcattcag cttttttata ctaacttgag
      601 cgaaacggga aggtaaaaag acaaaaagtt gtttttaata cctttaagtg ataccagatg
      661 gcattgcgcc atctggcaga gtgattaact aaacatcgca gtaatcgagg cgcttgccag
      721 agagtggaaa tgaacgttaa acccgaccat cgcgccgctg gcaccttcat cgacatcaat
      781 acgttctata tccagcgcgt gaacggtaaa aatgtagcga tgagtttcgc ctttcggcgg
      841 tgctgcgcca tcgtacccgg ttttaccaaa gtcggtacgc gtctgcaaaa cgccgtctgg
      901 cattgctacc agaccagagc caaacccttg cggtaatacg cgggtatcag cgggtaagtt
      961 aacaactacc cagtgccacc agccggagcc ggttggcgca tccgggtcgt agcaggtgac
     1021 aacaaaactt ttcgttcccg caggaacatc atcccacgcc agatgcggtg aaatattatc
     1081 gccatcgtaa cccatgccgt taaagacatg acgatgcggc aatttatcgc catcgcgcag
     1141 atcgttactg atgagtttca tgaaccctcc tttcttgttt gcagaaagtg tagccagaaa
     1201 ccctcacgcg gacttcccgt tattggcaaa aaaatgtttc atcctgtacc gcgcggttaa
     1261 ccgctgcggt cagacgctgc aactgttgcg ggagaataat atagggcggc atcaggtaaa
     1321 tcagtttgcc aaaaggccgg atccagacac cctgttcgac aaagaatttt tgcagcgccg
     1381 ccatattcac cggacgagtg gtttcgacca caccgattgc ccccagtacg cgcacatcgg
     1441 caaccatttc ggcatcacgg gctggtgcca gttgctcgcg cagctgcact tcaatagccg
     1501 ccacctgctg ctgccattcg ccggattcga taatcgccag gctggcgttt gctgccgcgc
     1561 aggccagcgg attgcccata aaagttggcc catgcataaa gcagccggct tcgccgttac
     1621 tgatggtttc tgcaacctcg cgcgtggtaa gtgtggcgga aagggtcatt gtgccgccgg
     1681 ttaaggcttt accgaggcac aaaatgtccg gcgcgatttc tgcatattca caggcaaaca
     1741 gtttgccggt acgaccaaat ccggtggcga tctcgtcggc aatcagcaag ataccttcgc
     1801 gatcgcacat tttgcggatt cgttttaacc attccggatg gtacatgcgc atcccgcctg
     1861 cgccctggac aatcggctca atgatcaccg ccgcgatttc atgacgatgc gccgccatca
     1921 ggcgggcaaa gcccaccata tcgcgctcat cccattcgcc atccatgcgg ctttgcgggg
     1981 cgggagcaaa caggttttct ggcaggtagc ctttccacag actgtgcatt gagttatccg
     2041 gatcgcacac cgacatcgcg ccaaaggtat cgccatgata accattgcgg aaggtcagaa
     2101 aacgctggcg cgcttcgcct ttggcttgcc agtactgcaa cgccattttc atcgccactt
     2161 ccaccgctac ggaaccggag tccgcgagaa aaacgcactc cagcggttgc ggcgtcatcg
     2221 ccaccagttt gcggcacagc tcaatggctg gcgcatgggt gataccgcca aacatcacat
     2281 gcgacatggc atcaatttgc gacttcatcg ccgcattaag ctgcgggtga ttgtagccgt
     2341 ggatcgccgc ccaccaggac gacataccgt caaccaggcg tttgccgtca gacaaaatca
     2401 gctcgcaacc ttcggcgctc accaccggat aaaccggcag aggggaggtc atggatgtgt
     2461 atgggtgcca gatatggcgt tggtcaaagg caagatcgtc cgttgtcata atcgacttgt
     2521 aaaccaaatt gaaaagattt aggtttacaa gtctacaccg aattaacaac aaaaaacacg
     2581 ttttggagaa gccccatggc tcaccgccca cgctggacat tgtcgcaagt cacagaatta
     2641 tttgaaaaac cgttgctgga tctgctgttt gaagcgcagc aggtgcatcg ccagcatttc
     2701 gatcctcgtc aggtgcaggt cagcacgttg ctgtcgatta agaccggagc ttgtccggaa
     2761 gattgcaaat actgcccgca aagctcgcgc tacaaaaccg ggctggaagc cgagcggttg
     2821 atggaagttg aacaggtgct ggagtcggcg cgcaaagcga aagcggcagg atcgacgcgc
     2881 ttctgtatgg gcgcggcgtg gaagaatccc cacgaacgcg atatgccgta cctggaacaa
     2941 atggtgcagg gggtaaaagc gatggggctg gaggcgtgta tgacgctggg cacgttgagt
     3001 gaatctcagg cgcagcgcct cgcgaacgcc gggctggatt actacaacca caacctggac
     3061 acctcgccgg agttttacgg caatatcatc accacacgca cttatcagga acgcctcgat
     3121 acgctggaaa aagtgcgcga tgccgggatc aaggtctgtt ctggcggcat tgtgggctta
     3181 ggcgaaacgg taaaagatcg cgccggatta ttgctgcaac tggcaaacct gccgacgccg
     3241 ccggaaagcg tgccaatcaa catgctggtg aaggtgaaag gcacgccgct tgccgataac
     3301 gatgatgtcg atgcctttga ttttattcgc accattgcgg tcgcgcggat catgatgcca
     3361 acctcttacg tgcgcctttc tgccggacgc gagcagatga acgaacagac tcaggcgatg
     3421 tgctttatgg caggcgcaaa ctcgattttc tacggttgca aactgctgac cacgccgaat
     3481 ccggaagaag ataaagacct gcaactgttc cgcaaactgg ggctaaatcc gcagcaaact
     3541 gccgtgctgg caggggataa ggaacaacag caacgtcttg aacaggcgct gatgaccccg
     3601 gacaccgacg aatattacaa cgcggcagca ttatgagctg gcaggagaaa atcaacgcgg
     3661 cgctcgatgc gcggcgtgct gccgatgccc tgcgtcgccg ttatccggtg gcgcaaggag
     3721 ccggacgctg gctggtggcg gatgatcgcc agtatctgaa cttttccagt aacgattatt
     3781 taggtttaag ccatcatccg caaattatcc gtgcctggca gcagggggcg gagcaatttg
     3841 gcatcggtag cggcggctcc ggtcacgtca gcggttatag cgtggtgcat caggcactgg
     3901 aagaagagct ggccgagtgg cttggctatt cgcgggcact gctgtttatc tctggtttcg
     3961 ccgctaatca ggcagttatt gccgcgatga tggcgaaaga ggaccgtatt gctgccgacc
     4021 ggcttagcca tgcctcattg ctggaagctg ccagtttaag cccgtcgcag cttcgccgtt
     4081 ttgctcataa cgatgtcact catttggcgc gattgcttgc ttccccctgt ccggggcagc
     4141 aaatggtggt gacagaaggc gtgttcagca tggacggcga tagtgcgcca ctggcggaaa
     4201 tccagcaggt aacgcaacag cacaatggct ggttgatggt cgatgatgcc cacggcacgg
     4261 gcgttatcgg ggagcagggg cgcggcagct gctggctgca aaaggtaaaa ccagaattgc
     4321 tggtagtgac ttttggcaaa ggatttggcg tcagcggggc agcggtgctt tgctccagta
     4381 cggtggcgga ttatctgctg caattcgccc gccaccttat ctacagcacc agtatgccgc
     4441 ccgctcaggc gcaggcatta cgtgcgtcgc tggcggtcat tcgcagtgat gagggtgatg
     4501 cacggcgcga aaaactggcg gcactcatta cgcgttttcg tgccggagta caggatttgc
     4561 cgtttacgct tgctgattca tgcagcgcca tccagccatt gattgtcggt gataacagcc
     4621 gtgcgttaca actggcagaa aaactgcgcc agcaaggctg ctgggtcacg gcgattcgcc
     4681 cgccaaccgt acccgctggt actgcgcgac tgcgcttaac gctaaccgct gcgcatgaaa
     4741 tgcaggatat cgaccgtctg ctggaggtgc tgcatggcaa cggttaataa acaagccatt
     4801 gcagcggcat ttggtcgggc agccgcacac tatgagcaac atgcagatct acagcgccag
     4861 agtgctgacg ccttactggc aatgcttcca cagcgtaaat acacccacgt actggacgcg
     4921 ggttgtggac ctggctggat gagccgccac tggcgggaac gtcacgcgca ggtgacggcc
     4981 ttagatctct cgccgccaat gcttgttcag gcacgccaga aggatgccgc agaccattat
     5041 ctggcgggag atatcgaatc cctgccgtta gcgactgcga cgttcgatct tgcatggagc
     5101 aatctcgcag tgcagtggtg cggtaattta tccacggcac tccgcgagct gtatcgggtg
     5161 gtgcgcccca aaggcgtggt cgcgtttacc acgctggtgc agggatcgtt acccgaactg
     5221 catcaggcgt ggcaggcggt ggacgagcgt ccgcatgcta atcgcttttt accgccagat
     5281 gaaatcgaac agtcgctgaa cggcgtgcat tatcaacatc atattcagcc catcacgctg
     5341 tggtttgatg atgcgctcag tgccatgcgt tcgctgaaag gcatcggtgc tacgcatctt
     5401 catgaagggc gcgacccgcg aatattaacg cgttcgcaat tgcagcgatt gcaactggcc
     5461 tggccgcaac aacaggggcg atatcctctg acgtatcatc tttttttggg agtgattgct
     5521 cgtgagtaaa cgttattttg tcaccggaac ggataccgaa gtggggaaaa ccgtcgccag
     5581 ttgtgcactt ttacaagccg cgaaggcagt aggttatcgg acggcaggtt ataaaccggt
     5641 cgcctctggc agcgaaaaga ccccggaagg cttacgtaat agtgacgcgc tggcgttaca
     5701 gcacaacagc agcctgcagc tggattacgc aacggtaaat ccttacacct tcgcagaacc
     5761 cacctcgccg cacatcatca gcgcgcaaga gggcaggccg atagaatcat tggtaatgag
     5821 cgccggatta cgcgcgcttg aacaacaggc tgactgggtg ttagtggaag gtgctggcgg
     5881 ctggtttacg ccgctttctg acactttcac ttttgcagat tgggtaacac aggaacaact
     5941 gccggtgata ctggtagttg gtgtgaaact cggctgtatt aatcacgcga tgttgactgc
     6001 gcaggcaata caacacgccg gactgactct ggcgggttgg gtggcgaacg atgttacgcc
     6061 tccgggaaaa cgtcacgctg aatatatgac cacgctcacc cgcatgattc ccgcgccgct
     6121 gctgggagag atcccctggc ttgcagaaaa tccagaaaat gcggcaaccg gaaagtacat
     6181 aaaccttgcc ttgttgtagc tatcctgtat ttggttaaat tgcgagcgag atcgcgtctt
     6241 cgattgactg caatttaacc aattaaattc taaaataatc acgaaaaaaa ttttacttcc
     6301 gcc
//
LOCUS       DAFNBJ010000227         6302 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000227 DAFNBJ010000000
VERSION     DAFNBJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.227"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..715
                     /gene="pdeC"
                     /locus_tag="L2X49_004542"
     CDS             <1..715
                     /gene="pdeC"
                     /locus_tag="L2X49_004542"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBN4799518.1"
                     /translation="IDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQIT
                     DYVIDNVFLDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFE
                     VTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVET
                     LTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPP
                     QVFMQWMEQLPARELTRGQ"
     gene            complement(718..1041)
                     /gene="soxS"
                     /locus_tag="L2X49_004543"
     CDS             complement(718..1041)
                     /gene="soxS"
                     /locus_tag="L2X49_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBN4799519.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1127..1591
                     /gene="soxR"
                     /locus_tag="L2X49_004544"
     CDS             1127..1591
                     /gene="soxR"
                     /locus_tag="L2X49_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBN4799520.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2137..3486
                     /gene="ghxP"
                     /locus_tag="L2X49_004545"
                     /gene_synonym="yjcD"
     CDS             2137..3486
                     /gene="ghxP"
                     /locus_tag="L2X49_004545"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBN4799521.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3637..5286
                     /locus_tag="L2X49_004546"
     CDS             3637..5286
                     /locus_tag="L2X49_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBN4799522.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5440..>6302)
                     /locus_tag="L2X49_004547"
     CDS             complement(5440..>6302)
                     /locus_tag="L2X49_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBN4799523.1"
                     /translation="GGYDFSHLSLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTN
                     FYYAIMEKILFDNCILDDSNFAQIKMADGTLNACSAMHVQFYNAAMNRANIKNTFLDY
                     SNFYIAYMAEVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINL
                     IDSKLFCTWLTNTFLEMVICTDSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRV
                     NFDEASVQGMGISILRGEENIPIDSDTLVTRQKFFEEDCTSHTGMSQTEDNINAVAMK
                     ITADIMQHAD"
BASE COUNT         1436 a         1440 c         1685 g         1741 t
ORIGIN      
        1 aatcgatatc aaaacagaaa aatgtatcgg cgctgaagcg ttgttacgtt ggcttggtga
       61 gcaggggcaa ataatgaatc cggcagagtt tattccgctg gcagaaaagg aggggatgat
      121 agaacagata actgattatg ttattgataa tgtcttcctc gatctgggcg cgtacttggc
      181 aacacatgcc gatcgctatg tttctattaa cctgtcggcc tccgattttc atacgtcacg
      241 gttgatagcg cgaatcaatc agaaaacaga gcaatatgcg gtgcgtccgc agcaaattaa
      301 atttgaagtg actgagcatg catttcttga tgtcgaaaaa atgacgccga ttattctggc
      361 tttccgccag gctggttacg aagtggcaat tgatgatttt ggtattggct actctaactt
      421 gcataacctt aaatcattga atgtcgatat tttgaaaatc gataaatcgt ttgttgaaac
      481 gctgaccacc cacaaaacca gtcatttgat tgcggaacac atcatcgagc tggcgcacag
      541 cctggggtta aaaacgatcg ctgaaggcgt cgaaactgag gagcaggtta actggctgcg
      601 caaacgcggc gtgcgctatt gccagggatg gttttttgcg aaggcgatgc cgccgcaggt
      661 gtttatgcaa tggatggagc aattacccgc gcgggagtta acgcgcgggc aataaaacta
      721 caggcggtgg cgataatcgc tgggagtgcg atcaaactgc cgacggaaaa cgcgggagaa
      781 ggtctgctgc gagacataac ccaggtccat tgcgatatca aaaatcggac gctcggtggt
      841 gcgcaactca acggcggcca gtaacaggcg acgttggcga atgtaatcgc caagcgtctg
      901 atgcgtcacc gtgcggaaca ttcgttgcaa gtaccacttt gaatagcctg atttttttgc
      961 gactacatca atgttaagcg gctggtcaat atgctcgtca atccatgcga taagatcctg
     1021 aataattttc tgatgggaca taaatctgcc tcttttcagt gttcagttcg ttaattcatc
     1081 tgttggggag tataattcct caagttaact tgaggtaaag cgatttatgg aaaagaaatt
     1141 accccgcatt aaagcgctgc taacccccgg cgaagtggcg aaacgcagcg gtgtggcggt
     1201 atcggcgctg catttctatg aaagtaaagg gttgattacc agtatccgta acagcggcaa
     1261 tcagcggcga tataaacgtg atgtgttgcg atatgttgca attatcaaaa ttgctcagcg
     1321 tattggcatt ccgctggcga ccattggtga agcgtttggc gtgttgcccg aagggcatac
     1381 gttaagtgcg aaagagtgga aacagctttc gtcccaatgg cgagaagagt tggatcggcg
     1441 cattcatacc ttagtagcgc tgcgtgacga actggacgga tgtattggtt gtggctgcct
     1501 ttcgcgcagt gattgcccgt tgcgtaaccc gggcgaccgc ttaggagaag aaggtaccgg
     1561 cgcacgcttg ctggaagatg aacaaaacta aagcgccaca agggcgcttt agtttgtttt
     1621 ccggtctttg tctttctctc tatcccgctg gtacacagga gggtttcccc cgacgtcaac
     1681 acaccccatt cgagcacgtg gtggaggttc cggttggtgt tgatgcttta attgtatgtc
     1741 accggcattt cttcgccagt gtaaaagtat actttttatc cgcaatattt ttgtcatctc
     1801 agacgatttt ttatcgcaat cctgaacggt atacggctcg ataacgctgc aatcttgcgc
     1861 accgacgata acgtttgcgc atcaattgcc tggtttttca tcgtcaagac aataaaagag
     1921 aaaaaagcag caaacttcgg ttgaaaaagc cgctatgatc gccggataat cgtttgcttt
     1981 ttttaccacc cgttttgtat gcgcggagct aaacgtttgc ttttttgcga cgcagtaaat
     2041 tgtcgcaaac ctggagcagg aagataacgt ttcgctggca ggggattgtc cgccacgcat
     2101 cttgacgaaa attaaactct caggggatgt tttcttatgt ctacgccatc agcgcgtacc
     2161 ggcggttcac tcgacgcctg gtttaaaatt tcacaacgtg gaagcactgt ccgtcaggaa
     2221 gtggttgccg ggttaacaac gtttctggcg atggtctact cggtcatcgt cgttccaggt
     2281 atgttgggta aagcaggctt cccgcctgcg gcagttttcg ttgcaacctg tctggttgcc
     2341 ggactcggtt ctatcgtgat gggtctgtgg gctaatctgc cgttggcgat tggttgcgcc
     2401 atctccctga cggcgtttac cgcattcagc ctggtgctgg ggcaacatat tagcgtacct
     2461 gtcgcgctgg gtgccgtgtt cctgatgggg gtgctgttta cggtaatttc tgccacgggt
     2521 atccgtagct ggattttgcg caacttgccg cacggtgtgg cgcacggcac ggggatcggt
     2581 atcggtctgt tcctgctgct cattgccgct aatggtgtcg gtctggtaat taaaaacccg
     2641 cttgatggtc tgcccgttgc gctgggtgat ttcgcgacct tcccggtgat tatgtcactg
     2701 gtaggtctgg cggtgatcat cggcctggaa aaactgaaag tccctggtgg cattctgctg
     2761 accattatcg gtatctcaat tgtcggtttg atcttcgatc ctaacgtcca tttctccggc
     2821 gttttcgcca tgccttcatt gagcgatgaa aacggcaatt cactgattgg cagcctggac
     2881 attatgggcg cgctgaatcc tgtagtcctg ccaagcgttc tggcgctggt aatgacggca
     2941 gtatttgatg ccaccggaac tatccgtgcc gtcgccggcc aggcgaacct gctggataaa
     3001 gatgggcaaa ttatcgacgg tgggaaagca ctgaccactg actccatgag cagcgttttc
     3061 tctggcctgg tgggtgcagc tccggcagcg gtatacatcg agtctgcggc gggtacggcg
     3121 gcgggcggta aaaccggttt gacggctatc accgttggcg tgctgttcct gctgattctg
     3181 ttcctctctc cgctctctta cctcgttccg gggtatgcaa cggctccggc gctgatgtac
     3241 gttggcctgc tgatgctgag caacgtggcg aaaatcgact ttgctgattt tgttgatgcg
     3301 atggcgggtc tggttacggc ggtattcatc gtgctgacct gtaacatcgt aacaggcatc
     3361 atgatcggct tcgcgactct ggtgattggt cgtctggttt ccggcgaatg gcgcaagttg
     3421 aacatcggta cggtcgttat cgccgtggcg ctggtgacct tctatgcggg tggctgggct
     3481 atctaatctt tcatcggatt ctgaaaacgg gtggcaatgg ctgcccgttt ttattttctc
     3541 cgcacattgt gtgagctttt gcgatattct gaaaaaatga gaattcaggc ataacgtctt
     3601 tctcagatca taaaaaacat cgcaaacagg gaacgcatgg aaatcttctt caccatactg
     3661 ataatgaccc tcgtggtctc gctgtccggg gtggtcactc gtgtcatgcc ctttcagatc
     3721 ccgcttccgc ttatgcaaat cgccatcggt gcgctactgg cgtggccgac gtttggtttg
     3781 catgtggagt ttgatcctga actcttttta gtcttgttta tcccgccgtt gctgttcgct
     3841 gatggctgga aaacgccgac ccgtgaattt ctcgaacatg gtcgagagat tttcggcctc
     3901 gcgctggcgc tggtggtggt caccgtggtc ggcattggct tccttattta ctgggtggtg
     3961 ccgggcattc cgctgatccc cgcctttgcg ctggcggcgg tactttctcc gaccgatgct
     4021 gtggcgctct ccgggattgt tggcgaaggg cgcatcccga aaaaaatcat gggcattttg
     4081 cagggcgaag cgttgatgaa tgacgcttcc ggcctggtgt cgttgaagtt tgccgtggca
     4141 gtggcgatgg ggacgatgat cttcaccgtc ggcggtgcga cggtcgaatt tatgaaagta
     4201 gccattggtg gtattcttgc cggttttgtg gtgagctggc tgtatggtcg ctcgctgcga
     4261 ttcctcagcc gctggggcgg tgatgaaccc gcgacgcaga tcgtcctgct gttcctgctg
     4321 ccattcgctt cttatttgat tgccgaacat attggcgttt ccggcatcct cgctgcggtt
     4381 gccgccggga tgaccatcac ccgctccggt gtgatgcgcc gtgcgccact ggcaatgcgc
     4441 ctgcgcgcaa acagcacctg ggcgatgctg gaatttgtct ttaacggcat ggtgttcctg
     4501 ctgttaggtc tgcagctgcc aggtattctg gagacgtcgc tgatggcggc agaaatcgac
     4561 cctaacgtcg aaatctggat gctgtttacc gatattattc tgatatatgc ggcgctgatg
     4621 ctggtccgtt tcggctggct gtggacgatg aaaaagttca gcaaccgctt cctgaagaag
     4681 aagccgatgg agtttggttc gtggaccaca cgagaaatcc tgatcgcgtc tttcgccggg
     4741 gtgcgtgggg cgatcactct ggccggtgtg ctctctatcc cgctgctctt gccggatggt
     4801 aacgtcttcc cggcgcgcta tgagctggtg ttcctggcgg ctggcgtcat tctcttctcg
     4861 ctgtttgtcg gcgtggtgat gttgcctatt ctgctacaac acattgaagt cgcggaccat
     4921 tcgcaacaat tgaaagaaga acgtattgcg cgagcggcaa cggcagaagt ggcgattgtg
     4981 gcgatccaga aaatggagga gcgtctggcg gcggataccg aagagaatat cgataaccag
     5041 ctgcttacgg aggtcagttc tcgcgtcatt ggtaacctgc gtcgtcgcgc cgatggacgt
     5101 aacgacgttg aaagttccgt gcaggaagag aaccttgagc gtcgcttccg tctggcggca
     5161 ttgcgttctg aacgtgctga actgtaccac ctgcgcgcca cgcgggagat cagcaacgaa
     5221 acgctgcaaa aattactgca cgatctcgat ttgcttgaag cgttgctaat tgaggaaaat
     5281 cagtaagccg aaagcgacgt aaccaggccc gaggtaatct gaaaaaggtg caatcttttg
     5341 cacctttaac aggttagctc gggctaacat tcatgattct aaaacaaata agttatctct
     5401 cttgtaaagt caattcattg gctgttcttc gatttaacct caatctgcgt gttgcataat
     5461 atctgcagta atcttcatag cgactgcatt aatattatcc tcagtttgtg acatgccagt
     5521 atgagaggta caatcttctt caaagaattt ctgtcgtgtt accagggtat cactatcaat
     5581 ggggatattt tcctccccac ggagtatgga aatgcccatt ccctggacgc tagcctcatc
     5641 aaaattaacc cgatagagac gggtattaaa catacaggct tttgttaaaa tagaacagtt
     5701 gaaatggcag ttgcttaaat tggcattatt aaaattaaca ttagccatat tagagtcggt
     5761 acatataacc atttcgagga atgtatttgt tagccacgta caaaagagtt tgctatcaat
     5821 taagtttata ttctggagga ttgctttgtt cagattgacg cgagacagat cagcatgttc
     5881 aaagttaatt aaatcgagtt tagagaaact aaggtcggct ttaaataaat taacatatgg
     5941 cgctattact ttataaagat ttacctcagc catgtacgct atataaaaat ttgaatagtc
     6001 aagaaaggta tttttaatat tggctctatt cattgctgca ttgtagaatt gaacatgcat
     6061 agcggagcat gcatttagag ttccgtcggc catttttatc tgagcgaaat ttgagtcatc
     6121 gagaatacaa ttatcaaaaa gtattttttc cattatggcg taataaaaat tcgttttata
     6181 catattacag tcctggaaaa tagcgttttg tagtctgcaa cctgcaaaat tcgaattgga
     6241 taaatcttca tctttaatca cgataccttt caggctaagg tgtgaaaaat cgtaaccacc
     6301 at
//
LOCUS       DAFNBJ010000228         6267 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000228 DAFNBJ010000000
VERSION     DAFNBJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.228"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..349
                     /locus_tag="L2X49_004548"
     CDS             <1..349
                     /locus_tag="L2X49_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799524.1"
                     /translation="SIFAAIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDI
                     MSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFAGDLFKYESVCIAAMEHLKS
                     KATAPIQNGPLPA"
     gene            complement(353..1744)
                     /gene="tcyP"
                     /locus_tag="L2X49_004549"
     CDS             complement(353..1744)
                     /gene="tcyP"
                     /locus_tag="L2X49_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HBN4799525.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFATFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1877..2467)
                     /locus_tag="L2X49_004550"
     CDS             complement(1877..2467)
                     /locus_tag="L2X49_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBN4799526.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2630..3298)
                     /gene="hxpB"
                     /locus_tag="L2X49_004551"
     CDS             complement(2630..3298)
                     /gene="hxpB"
                     /locus_tag="L2X49_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBN4799527.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3445..3981
                     /locus_tag="L2X49_004552"
     CDS             3445..3981
                     /locus_tag="L2X49_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBN4799528.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4022..4882)
                     /locus_tag="L2X49_004553"
     CDS             complement(4022..4882)
                     /locus_tag="L2X49_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBN4799529.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSSNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4988..5278)
                     /gene="ghoS"
                     /locus_tag="L2X49_004554"
     CDS             complement(4988..5278)
                     /gene="ghoS"
                     /locus_tag="L2X49_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBN4799530.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5379..>6267)
                     /gene="pfkB"
                     /locus_tag="L2X49_004555"
     CDS             complement(5379..>6267)
                     /gene="pfkB"
                     /locus_tag="L2X49_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBN4799531.1"
                     /translation="SATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAI
                     FPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALNED
                     EFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQGIRCIIDSSGEALSA
                     ALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALG
                     VDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFGVAAGSAATLNQ
                     GTRLCSHDDTQKIYAYLSR"
BASE COUNT         1610 a         1568 c         1543 g         1546 t
ORIGIN      
        1 gagcattttt gctgcgattc aaaaaaatga gagtctccta aagtttcctt cgataatatc
       61 aatgcttaat atcaatcttc tttttaatcc actaaatctt ccgggaatgg gaagtggtgt
      121 gcttgaagat attatgagta ttccagatag ttcacttaga aaacgtctcg ggtatgaggt
      181 tttaagcttc tcgctacagg cacattcatt gagccaggaa tgtattgata agttggacat
      241 ttttttcgct ggcgatctgt ttaaatatga gtcagtatgc atcgccgcga tggaacattt
      301 aaaaagtaag gcaacggccc ctatacaaaa cgggccgttg ccagcataag aattaacggt
      361 gtgccagttc ggcgtcgtct tcgctatcca gaatggcttt atcggtttgc ttcagccact
      421 ggctggtcag cgtgccagct gtcatcgagc cactaacgtt tagcgccgta cggcccatgt
      481 cgataagcgg ttcaacggag attaacagcg ccaccagggt tactggcagg cccatcgcag
      541 gcagcacaat cagtgcggcg aaagttgcac caccaccgac accggcaacg cctgcggaac
      601 taacggtaac aataccgacc agcgtcgcaa tccacatcgg gtccagcggg ttaatgccaa
      661 ccgtaggcgc aaccatcacc gccagcattg ccggatacaa accggcgcag ccgttctgac
      721 caatggttgc accgaaagag gcggcgaaac tggcgatgga ttcaggaacg cccagacgac
      781 gcgtttgtgc ttccacattc agtgggatag acgcagcact ggaacggctg gtaaaggcaa
      841 acgtcagcac aggccatacc ttacggaagt acttcagcgg actcacgcca ttaatgccca
      901 gcagaatgcc atgcactgca aacataatca gcagaccgag gtaggacgcg acaacgaaac
      961 ttcccagttt gatgatgtct tgcaagttag aacctgcaac cactttggtc attagagcca
     1021 gaacgccgta aggggtcaac tgcatgacaa ggcgaaccag tttcatcacc cagctttgta
     1081 gggtatcgat agcggctaag acgcgttcac ctttcggcgc atcatccttc agcagtttca
     1141 atgcagctac gccgaggaat gtggcaaaaa ttaccacgct gataattgac gtcggattgg
     1201 ctccggtaag atcggcaaac gggtttttcg ggataaagga caagaccagc tgcggaacgc
     1261 tcagatcaga gactttacca acatagttac tttcaatggc gttcagacgt gcagtttctg
     1321 caccaccctg aaccagacct tcagccgtca aaccaaacag gttggtgacc agcacaccga
     1381 ccagcgccgc aatcagcgtg gtaaacaaaa gcgtaccgat ggtcagaaaa ctgattttgc
     1441 ctaactgaga tgcgttatgc agacgggcaa ccgcgctcag aatagaggcg aagactaacg
     1501 gcataacgat catttgcagc agttgaacat agccgttacc aacgatgtta aaccactgta
     1561 cagaatcttt aagtacctgg ctgtcagaac cataaatggt atgcagggca aggccaaaaa
     1621 ccacacccat caccagaccc accagcactt ttttcgccag actccactgt ttatggcggg
     1681 tctgagccag cgcaaacagc agtacaacga acaccacgat gttcgcaatt aatggaaagt
     1741 tcatccccgt tctcctgatc ttattatttc tatcactgcg tagcagtcat agtgttggcg
     1801 caagattaac agaactgtga gctggctctt atattcaaat ggaattgctt attccagaaa
     1861 ggtgattttt gcctttttag tattcaacct gatgcttaat aagccgatga aactggatct
     1921 gcaaggtatt gatcatttgc gatgaccaac gaacagcgct gttctcgggt aatgaatggg
     1981 gcatccatac cgaccagaca aataatgcca tgcagataaa acgttccagt tggttgccct
     2041 tttggggaac caggataggc aagcggaaac gccagcggca tggccagagc aggggaacac
     2101 cggcgggtgt cagcatatcg gcaagtatgt ggctcaaata acccagcacc attccttgta
     2161 gcgcatcagc cggaatgaac cagccttccg gaaccttaag gtaaaaggtt gccagcagcg
     2221 caaataccgc cagcagactg tgggtaaaac cacgatgccc aaaagcgcgg gcgatcggtt
     2281 ttgatatcca ttttaatcgc tgcccaagaa acgactttgg gtgatcgatg tccggtaaca
     2341 aacacgtcag gattgcggaa gggacaatat gccaccagtc accctgtgcc agcacgggcg
     2401 tcagctcggc atttttggca aataccgcac aagcaataga aaagagaagg tgaccttccg
     2461 ccgtcatgat aaaactcact aaactgtcga tacgtacagt ataggatttt tatacagtgc
     2521 acggaagtgg tgacggtgta actctttctc ttaaattgac gattaatccg tcagttgaat
     2581 gcaaattcag ataaagaacc aggggcagcg tgatcactgc ccctggtcat taaccgagaa
     2641 ggtcttttgc ggtgagttct gtcagcgatg agagtttgac gtttgctaat acaaaacgtg
     2701 gatcattttg cgcttctggc gcaggaacga cgatggaacg catgcgggct gctttagaag
     2761 cgatcatgcc atttaccgag tcttccagcg ccacgcaggt cagtgggtca acgcccagtt
     2821 ttgctgcgca gtcgagatat acttgcggat gcggcttgct gtaaggcagt ttttcagccg
     2881 aggcgagggc atcgaaactg tcgcgtaagt caaacatggt caacactttt tccagcatat
     2941 gtagtggtga cgcggaggcc agtcccacca ataaaccttg ttctttgcat aacgcaacag
     3001 cttcgcgcac gcctggtaat aatggacgtg tctcttcaac cagtgaaatg gcacgggcaa
     3061 taacccgttc tactacttcc tgacggcttg gcccattcca cggttgccgg gcgtaccaaa
     3121 gatcgaccac catatcgatg cgtaaaccta aggtgtccgg cagctcgtta cgacgggaga
     3181 tatccacccc caggcttgcc atcacatcca gttcggctcg atcccataaa ggttctgagt
     3241 cgataagtaa tccatccata tcaaaaattg cagcaagaat ctgacgcggg gttgacatta
     3301 caacctctcc tttagagcat tatgtgtaag gtattaagcc gtgcttatga ttaagcgcct
     3361 atcatatcgc ctttaaagaa tgggcgaaaa cagatatcta caggtaaact aagtgaaacg
     3421 acgcgtctta atcaaggaga actcatgacg tatcaacaag ctggacgcat tgctgttttg
     3481 aaaaggattt tgggttgggt gatttttata cctgcattga tctctacatt gatttctttg
     3541 ctgaaattta tgaatacccg gcaggaaaac caggaaggca ttaatgcggt catgctcgac
     3601 tttactcatg tcatgatcga tatgatgcag gcgaatacgc cttttttgaa tctgttctgg
     3661 tataactccc cgacccctaa ttttaatggt ggcgtgaacg ttatgttctg ggtgattttc
     3721 atcctgattt ttgtcgggct ggcgttgcag gattccggtg cccggatgag tcgccaggcg
     3781 cgcttcttgc gggaaggtgt tgaagatcaa ctcattctgg aaaaagccaa aggggaagag
     3841 gggctaacgc gtgaacaaat tgagtcccgt attgttgttc cgcaccatac tattttcctg
     3901 cagtttttct cactgtatat cctgccggtt atttgtattg ctgcaggtta tgtgttcttt
     3961 tctctgcttg ggtttattta atcaaaaggc cgcacggagc ggccattatc ctcaacccat
     4021 atcatgctgc taataatcta tccaatgact gctgagcaat aaccaaatgc tgaccgccaa
     4081 ataatcttgc acgatttaac agcgtgtaga gttggtaaac cggttgacgt tcaaggaaat
     4141 cggcaggtag cggggatact gactgatagc cgtcatagat ttgtggcggc tgttcagtat
     4201 gcagcggtaa catcgccagg tcgcactctc ggtcacccca gtagcaggca gggtcgaaaa
     4261 tgtacgggcc atccggaccc agcgcacagt tgctggacca taaatcgccg tgcaacagag
     4321 aaggctgcgg ttgatgcgag gccagacgct gctgaatatg ctcgacgatg gcgtcgatat
     4381 tgccgaaagc gatccctttc tctgctgcca gttccaactg ccagccaatc cgttgttcag
     4441 caaaaaacgt tgaccagcga cgttgccagg tgttgggctg tggagttgtg gagagcgagt
     4501 tatcaaaatc gaggccaaat tgtggttggt cactccattg atgtaaacgc gcaatttgct
     4561 gaccaagaat aaatgcgcta tgcgcatcca gcggacgagg tgggagataa tccatcacca
     4621 gaaaactgta gtcacggtca gcgcctactg cccaaacctt aggcacggtg acggttttac
     4681 tacgcgacag taactccagt tggtcggctt cggcggtaaa accgggaagc agttcccttt
     4741 catcacattt gacgaaaaag tcatgtcctg catagcgcaa atgccatgcg gcatggactt
     4801 ctccgccagg cagttcatta cgcagttcga tttcgccttc acctaactgc tcgctcaaaa
     4861 gacgactgat tgcctgccac atgatgtctc tcccatgttg tctgccagat cataaggtta
     4921 gcgcaagaat gcggtgtaaa aacacgagct aacgcacatc acagactgat tattgcccaa
     4981 aaaccattta ttttctgttg ctattccatt cctcccaggt cgtgatggtg atttcaggat
     5041 ctttgccggt tgcactttgg gtaagccccg aaaccagttc tctaatttct tcttcacttt
     5101 gggtactgat aagtccaaaa gtgttagtcc ccagctcatg gatatttccc tcatcatccg
     5161 tcatggtgag caaaaaaccg tcgcgagtca ggtaattatt caactcgtta atttcagtca
     5221 atgtatcctc atgcaacatg acggttatga cataacggac aagatcgcca ctggccatag
     5281 ttcacccctt tgttatcaca tgacttttga gcatagtcgg agaaacgcgt tgccgacagg
     5341 ttggtggtga ttcccccaat gctgggggaa tgtttttgtt agcgggaaag gtaagcgtaa
     5401 attttttgcg tatcgtcatg ggagcacaga cgtgttccct gattgagtgt ggctgcactc
     5461 cccgcagcta cgccaaaacg gaccatctct tcaagagagg cattttctgc cagtttcagt
     5521 gtcatcgcgc cgaccatgct gtcaccagcg ccaacggtac tctggctttt caccggtggt
     5581 ggcaccacct gaatgcagtt ttcactatca acacccagcg ctccttgtgg accaagggaa
     5641 acgacaaccc gtttggcctt gccgctatta acgatttcct gcgcggcttt gcggacatcg
     5701 tctggctggg tgagttcgcg attcaccagc gcactgagtt ctttttggtt aggcttaacc
     5761 aactcgatgt taccaatcgc cagtgctgca cttaacgctt cgccagaact gtcgatgatg
     5821 cagcggatcc cttggttttg cgcagcggaa atcagttggg ttaatttttc cagcttcaca
     5881 cctggcggca ggcttccgct tatgaccagg atggccccgg attcaatttc cagaacttgc
     5941 tcttcaagct ggcgaaactc atcttcattt aatgccgcgc ctggcataac aaaacgatac
     6001 tgctcaccgc ttgcttccac atgtacgtgt aaattctgcc gggtccagtc tttggcttct
     6061 acagtagcga cggggacatt ttcatccgcc aacagagaaa ccaggtgttc gccggtcgcg
     6121 ccacccgccg ggaagatcgc tgtggcactg cctccaagat gggcaatggc gcgggcgacg
     6181 ttgatgccgc cgcccccggg ttcgaacacc ggtgcggtac agcgcagttt tccttcggga
     6241 taaatttgcg gggtaattgt tgcgcta
//
LOCUS       DAFNBJ010000229         6207 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000229 DAFNBJ010000000
VERSION     DAFNBJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.229"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(104..2497)
                     /locus_tag="L2X49_004556"
     CDS             complement(104..2497)
                     /locus_tag="L2X49_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HBN4799532.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSTLS
                     NLITLYAADNEQEQLRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQR
                     FNSDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCP
                     PVENLNKSGGDLPDIKTMDEKELQEYLHNMGQKERRELTARLRLVKPKRKKAYIQSIL
                     EQQRLQLEAELTTRGFEGSASEIDLLLRGGSIPSGAGLRIFYRNQRLQEDDKWRQWY"
     gene            complement(2494..3351)
                     /locus_tag="L2X49_004557"
     CDS             complement(2494..3351)
                     /locus_tag="L2X49_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HBN4799533.1"
                     /translation="MSTILKWAGNKTAIMPELKKYLPAGPRLVEPFAGSCAVMMETDY
                     PSYLVADINPDLINLYKKVAADCESFISRARVLFEIANREVAYYNIRQEFNCSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKELRTFAEKAQRATFIC
                     ASFDETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKVKRSIGVAAGEGKSATEIIAVSGPRCWMGFDYSRGVD
                     SSAVYGVRA"
     gene            complement(3348..3575)
                     /locus_tag="L2X49_004558"
     CDS             complement(3348..3575)
                     /locus_tag="L2X49_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBN4799534.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKSPGVSRVLCIECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            complement(3575..3808)
                     /locus_tag="L2X49_004559"
     CDS             complement(3575..3808)
                     /locus_tag="L2X49_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HBN4799535.1"
                     /translation="MRNIETLTTKTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHISSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            complement(3876..4217)
                     /locus_tag="L2X49_004560"
     CDS             complement(3876..4217)
                     /locus_tag="L2X49_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HBN4799536.1"
                     /translation="MAINGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            complement(4335..4631)
                     /locus_tag="L2X49_004561"
     CDS             complement(4335..4631)
                     /locus_tag="L2X49_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="HBN4799537.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWITGEDGKRWHPCHSQDELL
                     SELTTRKRRKSKCMRQKVKWFISFVTEGRVIQYLKMICSVAIRHCRAMAVTFSR"
     gene            complement(4639..5148)
                     /locus_tag="L2X49_004562"
     CDS             complement(4639..5148)
                     /locus_tag="L2X49_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HBN4799538.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTMT
                     GLGASFGLL"
     gene            complement(5181..5402)
                     /locus_tag="L2X49_004563"
     CDS             complement(5181..5402)
                     /locus_tag="L2X49_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBN4799539.1"
                     /translation="MTPNISITLNTPHVTIERYSELTGLSIDTINDMLADGRIPRHRL
                     RKDKKREKVMINLAALTVDALTDCNVVFN"
     gene            5548..>6207
                     /locus_tag="L2X49_004564"
     CDS             5548..>6207
                     /locus_tag="L2X49_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HBN4799540.1"
                     /translation="MQNRGGQSVIDRILKAYGFSSRQAFCNHLGISQSTMANRYARDT
                     FPADWVVICSMETGVPVEWLAFGTDTEKGSITNNAEKSHNNCDSKHQHLNREQDIQNE
                     NSFTINQGGKAAIERIVLAYGFKTRQALADHIGVSKSTLANRYMRDTFPADWIIQCSL
                     ETGASLTWLTTGNGAMFEKPRNDTITIPYHKIIDGSLAQETFLTFDSKLLEGTFLQPL
                     AV"
BASE COUNT         1579 a         1575 c         1425 g         1628 t
ORIGIN      
        1 tataaacagt ataataggaa cagaaaaatg taaaactgtt ttttgtcagt taattggatg
       61 tactgatgtc ggtcaataaa gcacaaaatg ttaaacagca gccttagtac cattgacgcc
      121 atttgtcatc ttcctgcagc ctctggttac ggtaaaaaat acgtaaaccg gcaccggatg
      181 gaatgctgcc gccacgcaga agcaaatcaa tctcagatgc actaccttca aaccccctgg
      241 ttgtcagttc tgcctcaagc tgcaggcgct gctgctccaa aatactctgt atgtatgctt
      301 ttttccgctt cggttttacc agtctcaacc tggctgtcag ctcccgccgt tccttctggc
      361 ccatgttgtg gagatattcc tgcagctcct tctcatccat ggttttaata tcgggtaaat
      421 caccccctga tttgttcaga ttttcaacag ggggacagtt attgccacga gtccaagggg
      481 cgcaagcgcc ctggtcggct gccgcctcct gaacgtcaac ggctttacga accattttcc
      541 acttcactgc atgagtgcag atcttgccct ctgcaatggg tgaccagatg ccataaatac
      601 gaatgccatg atcgccatag gcggtcggct cttcgttgat ttcataagcg gttctgatga
      661 ggtgatattt acggggaacc agtacgccgc cctgcttcat gatgtaggtg gcaaaacaac
      721 cagcatcagc agcagccaga atggcatcaa gacgcgggtt atccagtacc ggcgcacctg
      781 cttttttgtc accctgttgc cttgccgcct gaccagccag caagcgaagt tcacggtaag
      841 cctgacgccc cggaatacca aagaagcgga attgctgaac acgatgcaga gacgcccagg
      901 cattcacgta ttcagcgtta tcacgcagag atttacccgt ttccttgctg atctcgccag
      961 ccagaccacg accgtcaatg ttcttactga tatatttcgc gatgtagctt gtcggcgttc
     1021 ctttgcgcgg gttaatcaac tcagacttaa agcgcggccc agtgttattg cccagctcct
     1081 cgcggtcttc acggatggca aacttacgca gtaatgcagt gatggcacgg cggtcttttt
     1141 tgcgcatgaa acacaacagg tgccagtgaa ctgtgccatc atgatgcggc tcagccaccc
     1201 gcacgccata ccagcgcaac ccggctttgt gcatcgcctt acgaaatgca gcaaacatac
     1261 cgaccagata atcgctgctt tgtcttaccg tcgcgtttgt ccaggtcggg tttggtctgc
     1321 cgttatttag cgtggaatgg aaacgcgacg gacaggtgat agtgtagaaa acggcgcagt
     1381 caccgcgcat ttccgcgata agctccagac ctttaacaca ggccatcatc tcattgcggc
     1441 gatgcgcagg gttgctgctg ctggcgttta ccacatcctc catgtccagc gtgtcgccgt
     1501 cttcgttcac cagttcatga gaacggaaaa actccagcga cttacgacgc tgctcacgtt
     1561 tatgcatcac ggcttcatag ctgacataag gagatgcttt tttgctgacc aggcaaacag
     1621 cgcgcaactg ctcttcccgc cattcgcaac gcatcttcca taatttccga taccaccagt
     1681 cggcgcacaa catacgcgcc agcgaacccg gaatgagttc atagggcacg ggtttacggc
     1741 ggtttctttt ccgacggagt tgctcaaacg caggtgggat gacatccaga cgcagggttt
     1801 ccgctgccac cttttcccat gtcttgcgga tttcttctgg cttaacgtca tcggtggcat
     1861 acaaatcacc acaagctgca tcaaggcaca tactcatatg cgcagctacc agggtggaaa
     1921 ggcgtttcac ctgatcctga ctcatttcag gcaggatcag caggccgtcc agcccttcat
     1981 ggcttgccat aaagcgaaaa gatgcagata gctgactgtc gcgtacatgc tccagtcgtt
     2041 ccagacatgg cttaatcgtc tcacgtaaat agcgggaata agcctttggc ctgcccaggc
     2101 tgctgaagta ttcaatacgt tgcatcagcg gcttgctgat atgggaaggc tgggcgttga
     2161 cgtccgccag aatgaccatg tctgaattaa aacgctgctg ctcatgcgcc agctttgccc
     2221 ggctaatgag cttatcctgc tccatttcgc gctggacagg atcacgtgat tcattaaaga
     2281 aataacgctc ccagacctga tcactcagtg cctcgcggcg caactgttcc tgctcgttat
     2341 cggcagcgta cagagtgatc aggtttgaaa gcgtagaaac cggcgcaact tccgccgggt
     2401 ccagataagg gttaatggcc tttttcgggc tgttccatga gaatgctgcg gcagcctcgt
     2461 taaagccgca gcagttgttc atatcggcat gactcatgca cgtactccgt acacggcaga
     2521 actatccacg ccacgcgaat aatcaaatcc catccagcag cgcggcccgg aaacagcaat
     2581 gatttctgtt gctgatttac cctcgccagc tgccacaccg atgctgcgtt ttaccttgat
     2641 atagtggtga gtaaaattgc gatacagcga acggatcagg gatgtgtcac tgttagaaac
     2701 aatgaccgga tgtccttctg atgaccgatg ttcaagaacg gatgccaggt gatactggtc
     2761 atcttcagtg aaaccatcag tgtgatagcc ggaaaacgta ccgtcatatg gcggatcgca
     2821 atacaccaca tctcccgcct tcaacatcgc cagcgtttca tcaaagctgg cgcagataaa
     2881 cgtcgctcgc tgggcttttt ctgcaaatgt gcgaagttct ttttcaggga aatacggatt
     2941 tttataatta ccgtagggaa tgttgaaatg cccgctcttg ttatagcgac ataaaccacg
     3001 gtaaccgtga cgattgagat acaggaaata taccgctttc atgaaatcag taatttcagt
     3061 tgagcagtta aactcctgcc ttatgttgta ataagccacc tccctgtttg cgatctcaaa
     3121 taaaactctg gcgcgagata taaacgattc acaatcagcg gcaacctttt tatagaggtt
     3181 gattaaatca ggattaatat ccgcaaccag atagctggga taatccgttt ccatcatcac
     3241 agcacaggaa cccgcgaaag gttcaaccag tcgcgggcca gcaggaaggt attttttcag
     3301 ttctggcata atggcggttt tatttcccgc ccatttcagg atggtgctca tacagcacct
     3361 ccgttgtaat gtttgccttt cagttctgcg atttcctgac aggtaatgca aagctgcact
     3421 cccggaatgg cgcggcgtcg tgctggcgga attggcgctt cacattcaat gcaaagtacg
     3481 cgtgacacgc ccggtgattt ggcacgggct gcacggatat ggcgctggcg ttcttcttca
     3541 acgcgctgct gtacgagatc cattgcatca gccattagtg gatctcctgc gcttcgttct
     3601 ggattgcttc agcagtcacg cgcagcagtt ctgccgcttc cacgtggttt agctggcggg
     3661 atgagatatg acacgccagg ctatcaaggc gagcagccat tgcttcagcc cttgcccggc
     3721 gttcttccag acgagcctct gtcagtaaaa tattaagccc tgcgtcatcc ggtccggttt
     3781 tagtcgtgag ggtttcaata ttacgcataa tcaattctcc tgaatttaga taaagggatg
     3841 cccggcgggt ttacgccatt aatttcatta gttggttaat tcggcatggt tagccgtctg
     3901 ggaaataagc tcaccactgc acgaaaatga ttcattgctt taatcagctc ccgcttttcg
     3961 tcagtggtca gctcattaat gctgatgcta tgacgttcag ctggaatttt tgccataaag
     4021 aatatggcag ccagtgcccg tttattttgt tcattattga tatcccgtgg atcacgcata
     4081 tctttaataa accgctcaag ctctgactca atattcaggc caaaaacttt cgcccttaac
     4141 tccgcaatgt gattaagtcc attcaggcgt tcaccggggc ttaatggaac agttgctgca
     4201 gcgccattaa ttgccatact tcatatcccc caaacgcagc tatcgttctt tgttcttacg
     4261 gtaacgctca agaggagata cattttttcg tatcgtctct ttaacctgct ctccccgtaa
     4321 aaacgtccca tcctttaacg tgaaaaagta actgccatcg cccgacaatg acggatagca
     4381 acagagcaaa tcatcttcag gtactgaata actctcccct ctgtaacgaa actgataaac
     4441 cacttcactt tctgccgcat acatttggac tttctccgtt tcctcgtggt caattcagac
     4501 agcaattcat cttgtgaatg acatggatgc cagcgttttc catcctcacc cgtgatccag
     4561 ccgtgaccgt agtgcattgc cgggctttgt tttaccagca gcgatgcaaa tgatggttct
     4621 ttcgtcagca taaacacctc acagcaaacc gaatgaagca ccaaggccag tcatggtatc
     4681 aactgcactc gccatcgcag gattagcctg taaacgggct tgcaatgaaa cagcagccag
     4741 cgccatcagt cgtgttacag agttaatgct gctgatagca tcacgacgac ctgcactggt
     4801 ttttacatcg ccagataccg cacctgcagc aacacgcccg atctctgcgg ttgcactcat
     4861 gacgtaatgc ggtagtttct cttttgccac ctcattaatc ggaacacatg gcagacaatg
     4921 aatctgtgcc agaaaaccat ctaccagcgt tgaatcttca gtcagatcgg taagcaacca
     4981 aatttctggt gcggttaata aatgaggttg agctgggttc agtttgttcc gcagaatctg
     5041 cacattcatg cctgcacgtt ctgccagttg caccagattg tggcgcagtg cgaatgcacg
     5101 acaggcttca tcaaaatgtg gatgtttgga aacttggtaa tcaaacatgg tcgacacctc
     5161 tgatgtatcc caaaatggaa ctagttgaat acaacattgc aatcagtaag tgcatcaacg
     5221 gtaagagcag caaggttgat cattaccttt tctcttttct tgtctttccg aaggcgatgc
     5281 cgagggatgc gaccgtcagc cagcatatcg ttaattgtgt cgattgaaag accagtaagt
     5341 tcgctataac gctcaattgt gacatgtggc gtattcaggg ttattgaaat gttaggggtc
     5401 atgatgcaac atctcctatt ggcttgtggt gagtcagttt taatcgtggc tttaacttca
     5461 catttcggag aataggatca caaatcggtt atgtcaacac atgaaatcac atttcgccat
     5521 gtggacgaaa aaaagaaatc cttaatcatg cagaatcgcg gagggcaatc ggttatagat
     5581 cggatactga aagcctatgg tttttcttcc cgacaagcat tctgtaatca cctaggtata
     5641 tcgcaaagta caatggcgaa caggtatgcc cgtgacactt tccctgctga ttgggttgtt
     5701 atctgtagca tggagactgg agtgccggtc gagtggttgg catttggcac tgataccgag
     5761 aagggaagca ttacaaataa tgcagaaaaa agtcacaaca attgtgacag caagcatcaa
     5821 catctcaata gagaacaaga catccaaaat gagaactctt ttactattaa ccaaggtgga
     5881 aaagcagcaa tagagcgaat cgttttggct tatggattta agacaagaca agctttagct
     5941 gatcatattg gtgtatcaaa aagtacatta gccaatcgtt acatgagaga tacctttcct
     6001 gctgactgga ttattcaatg ctcactggaa accggtgctt cattaacatg gctaaccact
     6061 ggtaacgggg caatgtttga aaagcctcga aacgatacta tcactatccc atatcataaa
     6121 ataattgatg gatctcttgc tcaagaaacc ttcttgactt ttgactctaa gttgttagaa
     6181 ggaacctttc tgcaaccttt agcagta
//
LOCUS       DAFNBJ010000230         6126 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000230 DAFNBJ010000000
VERSION     DAFNBJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.230"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            67..843
                     /gene="exuR"
                     /locus_tag="L2X49_004565"
     CDS             67..843
                     /gene="exuR"
                     /locus_tag="L2X49_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBN4799541.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1188..1850
                     /locus_tag="L2X49_004566"
     CDS             1188..1850
                     /locus_tag="L2X49_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBN4799542.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1854..2237
                     /gene="mzrA"
                     /locus_tag="L2X49_004567"
     CDS             1854..2237
                     /gene="mzrA"
                     /locus_tag="L2X49_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBN4799543.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2384..2752
                     /locus_tag="L2X49_004568"
     CDS             2384..2752
                     /locus_tag="L2X49_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HBN4799544.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2790..3095
                     /locus_tag="L2X49_004569"
     CDS             2790..3095
                     /locus_tag="L2X49_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBN4799545.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3098..3502
                     /locus_tag="L2X49_004570"
     CDS             3098..3502
                     /locus_tag="L2X49_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBN4799546.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3492..3791
                     /locus_tag="L2X49_004571"
     CDS             3492..3791
                     /locus_tag="L2X49_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBN4799547.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3977..4369
                     /locus_tag="L2X49_004572"
     CDS             3977..4369
                     /locus_tag="L2X49_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBN4799548.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4439..5425
                     /gene="yqjG"
                     /locus_tag="L2X49_004573"
     CDS             4439..5425
                     /gene="yqjG"
                     /locus_tag="L2X49_004573"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBN4799549.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISNYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5718..6083
                     /locus_tag="L2X49_004574"
     CDS             5718..6083
                     /locus_tag="L2X49_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBN4799550.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1527 a         1514 c         1552 g         1533 t
ORIGIN      
        1 ccaatggcat acccatgaat aaagtggtat gataacttcg tccgcattcc actccgggag
       61 acgcatatgg aaatcactga accacgccgt ttgtatcaac aacttgccgc tgacctgaaa
      121 gagcgcatcg aacagggcgt ctatctggta ggtgataaac tgcctgcaga acgctttatt
      181 gccgatgaaa agaacgtcag ccgtacggta gttcgtgaag ccatcatcat gctggaagtt
      241 gaaggctatg tcgaagtgcg taaaggttca ggtattcatg tggtttccaa ccagccgcgc
      301 catcagcagg cagctgacaa taatatggaa ttcgccaatt acggtccgtt tgagttgctt
      361 caggctcgcc agctcatcga aagtaatatt gccgaattcg cggcaactca ggtaacgaaa
      421 caggacatca tgaaactgat ggccattcag gaacaggcgc gcggcgaaca atgcttccgt
      481 gattccgagt gggatttaca gttccacatt caggtagccc tggcgacgca gaactccgcc
      541 ctggcggcca tcgttgaaaa aatgtggaca caacgtagcc ataacccgta ctggaaaaaa
      601 ctgcacgaac acattgattc ccgtaccgtc gataactggt gtgatgacca cgatcaaatc
      661 ctcaaggcgc tgattcgcaa agatcctcat gctgctaagc tggcaatgtg gcagcacctg
      721 gaaaacacca agatcatgtt attcaacgaa accagcgacg acttcgagtt caatgccgac
      781 cgctatctat tcgccgaaaa cccggtggtg catctcgata cggccaccag tggcagtaaa
      841 tgaaatacct gtccgacagg cgctgcgacg cgcctgtttg tctcgtgggt aagcgaaaag
      901 tataaagtgt cagcctgtgt aaatcctctc gccaccctcc cctgcattca gcaaaatcag
      961 actccacgga catgtaattt tgataacgaa caacgttgac ctttgttaca attagattca
     1021 atttgaattt atgtttttga atgctttctt atctcacgat ttaacaggga atagttcagg
     1081 ctgtgttgat gtatcaaacc cgcagaacat accaaaacag caataacatt gcggtagtgc
     1141 atcttttaaa accagcgtgg cgttaaccga ttcaccagga ataatgaatg gaacttttga
     1201 cccaattgct gcaagccctg tgggcgcagg attttgaaac cctggccaat ccatcgatga
     1261 ttggcatgtt gtattttgtc ttgtttgtaa ttttgttcct tgaaaacggc ttgcttccag
     1321 cggccttttt accgggcgac agtttactgg tattggtcgg cgtgttgatt gcgaaaggcg
     1381 cgatgggtta cccgcaaacg attctgctgc tgaccgttgc cgccagcctc ggctgctggg
     1441 tcagctatat tcaggggcga tggctgggca atacccgcac cgtacaaaac tggctatctc
     1501 atttacccgc gcattatcat caacgcgcac accatctttt tcataaacac ggtttatcgg
     1561 cgctgttaat tggtcgcttt attgcgtttg tcagaacact gctgccgacg attgccgggt
     1621 tatcagggct gaataacgcg cgctttcagt ttttcaactg gatgagcggt ctgctgtggg
     1681 tattgatcct gacaactctg ggttacatgc tcggcaaaac gccggtattt ttaaagtacg
     1741 aggaccagct gatgtcatgc ctgatgctgc tcccggtggt gctgctggtg tttggcctgg
     1801 caggttctct ggtcgtgtta tggaaaaaga aatatggaaa tcgggggtaa gggatgcaaa
     1861 tacctcgcat gtcgcttcgc cagctagcct ggtccggcac tgttttactt ctggtcggca
     1921 cgctgttgct ggcctggtcc gcggttcgcc agcaagagtc tacgctggcg attcgtgccg
     1981 ttcatcaagg cacaacgatg ccagacggtt tttcgatctg gcatcacctt gacgctcatg
     2041 gcattccttt caaaagtatc acccccaaaa acgacactct gttaattaca tttgactcca
     2101 gcgaccagag cgccgccgca aaagcggtcc tcgacagaac attgccccat ggctacatca
     2161 ttgcgcagca ggacaataac agtcaggcta tgcagtggct gacccggtta cgggataact
     2221 ctcatcgctt cggataactt cctgttatcc gaaacatatc actcactttg gtgatttcac
     2281 cgtaactgtc tatgattaat gaggcggtgg ttaacactac cgcttcgttg tctggacaat
     2341 cgttcctttg taataggtcc gaatcacaat ggaaggttca agaatgaaat accgcatcgc
     2401 tttagctgtt tctctctttg ctcttagtgc cggtagttat gccactaccc tgtgtcagga
     2461 aaaggagcaa aatatcctta aggagatcag ctatgccgaa aaacaccaaa accagaatcg
     2521 tattgacggt ctgaataaag ccctgagtga agtccgggcc aactgttcag atagccagct
     2581 gcgtgccgat catcacaaga aaatcgcaaa gcagaaagat gaggtggcgg aacgccagca
     2641 agatttagcc gaggcgaagc aaaaaggcga tgccgataag attgccaaac gcgaacggaa
     2701 actggcagaa gcgcaggaag agctgaaaaa gctggaagcg cgcgactact aactcacaat
     2761 agtcactact tactcacctg gagaaaacta tgtcgaaaga acacactacg gaacatctgc
     2821 gtgctgagtt gaaatccctt tccgatacgc tggaagaggt gcttagctca tctggcgaga
     2881 agtcgaaaga agagttgagt aagattcgta gcaaagcgga gcaggcactg aaacagagcc
     2941 gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca aacccgtgtc gcggcggcgc
     3001 gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt gggcattggc gctgcaatcg
     3061 gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg gcggacactc atcacgcgca
     3121 agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca tggttgaaat
     3181 ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag ctaatctctt
     3241 tcaactttta ctgatgctgg gcctgacgat gcttttcgct gcatttggtc ttatgagcct
     3301 gatggtgcta attatttggg cggttgatcc gcaatatcgc ctgaatgcga tgattgccac
     3361 caccgtggtg ttgctgctac tggcactgat tggcggtatc tggacgttac gtaaatcgcg
     3421 taaatccacc ttgctgcgcc atacacgcca tgagctggca aacgatcggc aactgctcga
     3481 ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg taaggcgcaa ctgcttagcc
     3541 agatccagca acaacggctg gatctttccg ccagtcgtcg tgaatggctg gaggcaacag
     3601 gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg ctcctgggcg ctggttggca
     3661 gtagcgtgat ggcgatctgg acgattcgcc atcctaatat gctggtccgc tgggccagac
     3721 gcggttttgg cgtatggagc gcctggcgtc tggttaaaac gaccctcaag cagcaacagc
     3781 ttcgcggtta acttccctct ggccggagca attccggcct tatccctcaa attttttgaa
     3841 gatttttgac agttttcctt gctaacaatc atcattcacc acgtttatga ttctctccat
     3901 cgacagcaac gacgctaata ccgcgccctt gcacaaaaaa acaatcagca gcctgagtgg
     3961 cgcagtggag agtatgatga aaaaattaga agatgttggt gtactggtag cgcgcatttt
     4021 aatgccgatt ctgtttatta ccgcaggctg gggaaaaatt actggctacg cgggtaccca
     4081 acaatatatg gaagcaatgg gtgtcccggg ttttatgctg ccgctggtga ttctgcttga
     4141 gttcggtggt ggtctggcaa tcctgttcgg tttcctgact cgcaccacag ccctgtttac
     4201 tgcgggcttt acgctgctga cggcattttt atttcacagc aactttgctg aaggcgtcaa
     4261 ctcgctgatg ttcatgaaaa acctgacaat ttctggcgga ttcctgctgc tggcaattac
     4321 cggtccgggc gcgtatagca tcgaccgcct gctgaataaa aagtggtaag cacgcgctat
     4381 acttaacgat aaaaaacgag gaggaagctc ctcgtttttg ctattggagg agagaaaaat
     4441 gggtcaactg attgacggcg tctggcatga cacctggtac gataccaaat ctaccggcgg
     4501 taaatttcaa cgttcagctt ccgcatttcg taactggctc actgccgatg gcgcacctgg
     4561 ccccactggc acaggcggtt ttatcgcaga gaaagatcgt tatcatctct atgtttcact
     4621 cgcctgcccg tgggcgcacc gcacgctgat catgcgcaaa ctcaaaggac tggaaccgtt
     4681 tatttccgtt tccgtagtga acccgctgat gctggaaaac ggctggacct ttgatgacag
     4741 ttttccggga gcaaccggag atacgctcta tcaacatgaa tttctgtatc agctttatct
     4801 ccacgccgat ccacactaca gcggacgagt tactgttccc gtgctgtggg acaaaaagaa
     4861 ccacaccatc gtcagcaacg aatcagcaga aatcatccgc atgttcaata ccgcgtttga
     4921 tgcgctgggc gcaaaagcgg gtgattacta cccaccagcc ctgcaaacga aaattgacga
     4981 acttaacggc tggatttatg acaccgttaa caacggcgtg tataaagctg gttttgccac
     5041 cagccagcaa gcctacgacg aggcggtggc gaaagtgttt gaatcgctgg cgcgactgga
     5101 gcagatttta ggccagcatc gttacctgac cggcaaccag ctaaccgaag ccgatattcg
     5161 cctgtggact acgctggtgc gttttgatcc agtgtatgtg acccacttca agtgtgataa
     5221 gcaccgcatc agcaattacc tgaatctgta tggcttcctg cgcgatatct accagatgcc
     5281 ggggatcgcc gaaactgtca atttcgatca catccgtaat cattacttcc gcagccataa
     5341 gaccatcaac cctacgggga ttatttcaat tggtccgtgg caggatctcg atgaaccgca
     5401 tggacgagat gttcgcttcg gttaaaaaaa ggcaccttac gaggtgcctc tgataattag
     5461 ctaagtgcat gttgttcatg tcggatgcgg cgtgaacgcc ttatcaaacc tacaaaacat
     5521 ttatattcaa tatgttgcaa tgaccgcgca ggccagataa gcgtagcgca tctggcgatt
     5581 ttgcgcttgt catcagtctt agcgcgcctt cacagcactt tattaattcc ttgaacgaat
     5641 atttactgcc atttcattca cgtctattct taatttgctg ctttaaaaac aagtgattga
     5701 gcaaaattga ggcaaaaatg gactggtatc tgaaagtact aaaaaattat gtcggtttcc
     5761 ggggtcgtgc gcggcgcaaa gagtactgga tgtttattct ggtcaacatc atctttacgt
     5821 tcgtgctggg gctgctggat aaaatgttag gctggcaacg cgctggtggc gaaggcatcc
     5881 tgacgacaat ctacggtatt ctggtgtttt taccgtggtg ggcagttcag ttccgccgcc
     5941 tgcacgacac cgaccgctcg gcgtggtggg cactgttgtt cttaatcccg tttatcggct
     6001 ggcttattat catcgtcttt aactgccagg cgggtacgcc aggcgaaaac cgctttggac
     6061 cagatccgaa actggaacct taaaacaaat ctgctcgccg gatgtctgac tcatccggct
     6121 tttatc
//
LOCUS       DAFNBJ010000231         6056 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000231 DAFNBJ010000000
VERSION     DAFNBJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.231"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..450)
                     /locus_tag="L2X49_004575"
     CDS             complement(<1..450)
                     /locus_tag="L2X49_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799551.1"
                     /translation="MEKTQSPAERFYESIYYSDESLEEDYLAELRNFSSDHWDTALRA
                     ARLSAAVKRFKTSEMLRFILEFVVPENAQEDVPDLTPLAAKRLCNSLFGRSGSQSILV
                     YVFGQAGRVHRSATCSPKTIEAIADLYRSDAERYWNSTLATIERVKHT"
     gene            complement(530..760)
                     /locus_tag="L2X49_004576"
     CDS             complement(530..760)
                     /locus_tag="L2X49_004576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799552.1"
                     /translation="MSYAHLFENCDHVIEGNILLDIASTPPVLKKALIFLIQSGLERE
                     AFNAQLTELIKHLEFDITAAELSAHLLTPGNA"
     gene            1145..1537
                     /locus_tag="L2X49_004577"
     CDS             1145..1537
                     /locus_tag="L2X49_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799553.1"
                     /translation="MDDKEQFTSLVAKHASRLTEEQLAGYDDCSQYGECVSPSYEVFR
                     GYRTRHTLDEFLELAISLNAIHPDEYLTDMLLKPHEVIGALADEGDQLNNATPVYIFP
                     DTGVYAAAVSETRVLDAWLCWPCYPANW"
     gene            1950..2153
                     /locus_tag="L2X49_004578"
     CDS             1950..2153
                     /locus_tag="L2X49_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799554.1"
                     /translation="MFSTEKKSEHLGQIIVIDPALLGQGIEVGPVVVFDLGHASKGVA
                     DLMRDAEKLDGLSPTTIANRLSR"
     gene            2559..3365
                     /locus_tag="L2X49_004579"
     CDS             2559..3365
                     /locus_tag="L2X49_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBN4799555.1"
                     /translation="MREPRPRHTLQVVCVPSLNIQDLGLTSFHSWLLSKGYDTTNARN
                     NRTCWAREGGWHLKRCRHLATGEDHFWFIAFNGTGGDIFPVKTQQDYRAAYRKLEAYG
                     YAPAVIEQLNTGVAYNLAYSRTPLKRPESATSELKRKPDVNIQGEPCERFVTERSSAA
                     QNKFKTLLIENFAGRCAVTGWVNGGVLVAAHIEHGTRYNPSNGILLTPTMHALFDADL
                     MGIDPSTLTVHFKPGIEAGELFEGKTLNPLVYDLDTDRLAARWADFIGAE"
     gene            3483..3890
                     /locus_tag="L2X49_004580"
     CDS             3483..3890
                     /locus_tag="L2X49_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799556.1"
                     /translation="MQNCLSGYLKATIVILSADLTGCDLANGAAESGVYQKYTNGPFK
                     EPTIVMSPGWRMDDHGREALIFGSSHCPDVNGNNTSEGGCVLIEEHSATVAVTVVDAT
                     NQSRRQETWTIERKKDRVIVKRPDNSFVMPWGK"
     gene            3937..4647
                     /locus_tag="L2X49_004581"
     CDS             3937..4647
                     /locus_tag="L2X49_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBN4799557.1"
                     /translation="MCVERPDGAFDIYTGRFIQFHQIDNSRRKIVEISDPDLVGMTYS
                     IPMVFNGGKFSGQGVTCLSLPAVTLIKVTFPSRTYQEGQEQITMSSPDQALFSEDVRK
                     NCAGTCVLTGVRGRQRTEAAHIKPRHAGGEPDVTNGILLRSDIHTLFDNWHFSIDPDS
                     MKARFSPDVLFVDKDLLQLEGKQIDFSRLQMPINIEHLRHHWNKFFNRHVNPFIKNAL
                     NKKINNYEKRPVNSNIHY"
     gene            4717..4901
                     /locus_tag="L2X49_004582"
                     /pseudo
     CDS             4717..4901
                     /locus_tag="L2X49_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5415..5819)
                     /locus_tag="L2X49_004583"
     CDS             complement(5415..5819)
                     /locus_tag="L2X49_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HBN4799558.1"
                     /translation="MSEALKSLNNIRTLRAQGRELPLEILEELLEKLSVVVEERRQEE
                     SSKEAELKARLEKIESLRQLMLEDGIDPEELLSSFSAKSGAPKKVREPRPAKYKYTDV
                     NGETKTWTGQGRTPKALAEQLEAGKTLDDFLI"
BASE COUNT         1612 a         1342 c         1509 g         1593 t
ORIGIN      
        1 ggtgtgtttg accctttcaa ttgtcgcaag agttgaattc cagtagcgtt cagcgtcaga
       61 acgataaaga tcagcgatag cctctatggt tttgggggaa caggttgcac tgcgatgcac
      121 acggcctgcc tgaccaaaga catagacaag aatggactgg ctaccagacc ggccgaacag
      181 ggagttgcaa agccgcttcg ctgcgagcgg tgtcagatcg gggacgtctt cctgagcgtt
      241 ctcgggaaca acgaattcca gaatgaagcg aagcatctcc gatgtcttga aacgctttac
      301 cgccgcagac aaacgggcgg cacgcagggc agtgtcccag tggtcgctgc tgaaattgcg
      361 gagctccgcg aggtaatcct cttccaggct ttcatcagaa taataaatgc tttcgtaaaa
      421 gcgctctgcg ggtgattgcg tcttttccat tggagaaacc attttgccta atgtatgcat
      481 acgttagcat gtagtgaaat taaaacaagg taaagacaaa gaaccgcatt caggcgtttc
      541 ccggagtaag cagatgggct gacaattcgg ctgcggtaat atcaaactca aggtgtttga
      601 tgagctctgt taattgagcg ttaaatgcct cccgctccag tccggattgg atgagaaaga
      661 tcagggcctt tttcagaacg ggcggagtac tcgcaatgtc gaggagaatg ttgccctcaa
      721 taacatgatc gcaattttca aaaaggtggg cgtaactcat tacgtgtatt ccatttatca
      781 ttttcaaatc gtgataaaaa ttagattacc agaacgtcgg aagcaaaaaa aagcattaag
      841 cagaaatgta tggtatccat ccttcctcca gctacccttg cgccctgata attttatcct
      901 gggggagggg gcattgatca ttacataccc gatcagaaaa gccgggaacc agaatgtcac
      961 ccggatatcg aagatgcaac gattcaatat tagtgtattt agcgtctcct ggcggcaaag
     1021 tcggtcggcc ggaactggta aactgtattc cacctcgccc gggctggcag gcgactgacg
     1081 tcttgtcggt tcatagccgg tggtcgcgtc tgacaataca ggcccctaca gtgtaaggat
     1141 tgtgatggac gataaagagc agtttacgag tctcgtggca aagcatgcct ccagactcac
     1201 cgaagagcag ctggccgggt atgatgactg ttcccagtat ggtgaatgcg tctcgccttc
     1261 atacgaagta ttccgggggt atcgtacccg ccacaccctg gatgaatttc tggagctggc
     1321 catatcgctg aatgccatcc acccggatga atatttaacg gatatgctgc tgaagcctca
     1381 tgaggtgatc ggtgctctgg ccgatgaagg cgaccagttg aacaacgcca ccccggttta
     1441 tatcttcccg gataccggcg tctatgcagc ggccgtcagt gaaacccgtg tgctcgatgc
     1501 ctggctttgt tggccatgct acccggcgaa ctggtaatgc gtccgttgga attgcaccgg
     1561 caggatacga atttaaccga ccccgtggcg ggagggttat cacaattgca tgtcctcatc
     1621 cctaaagtcc cctgccagac ccatacgtgc tgtcaggctt cgcctgattt gagatcagtg
     1681 caaatacttc agccgataac tttttgtttt ctttttattt tctccctgtt tcctcaaaat
     1741 tcgcgtgcgg cctggtcagg gcacgattgt cacatagggt gtattgaagc gggcaggatg
     1801 gtcgggtggg cacggcctgg gtttgagtcg gacactatgt gggatgagga catgcaaaat
     1861 attcgcttta tctgtgaaaa ctgttgtcta cgggcagtca ccagttcaag ttacaccatc
     1921 cacgttagct tttaataaga gtggttacga tgttcagtac tgagaaaaaa tcagagcatt
     1981 taggtcagat catagtgatt gatcccgcgc ttcttgggca gggtatagag gttggcccgg
     2041 tcgtagtgtt tgatttgggg cacgccagca aaggggttgc ggacttgatg cgcgatgctg
     2101 aaaagcttga tggtttatcc ccaacgacaa tagcaaacag actttcacga tgaccgaaca
     2161 aggtcgattc gggcaaggcg gcggaggggc acatctccca atgataatga ggttatgcaa
     2221 cctgttagtc accggcttgc ctgatgttat gattgacatc tcttttgacc ggtctgtagt
     2281 tgagcattaa ttcgtgagga atacggtatc agcggataaa agtagcatgt ttgctatcgc
     2341 ctccagctgt gaaaaccctc ggtgggtgca agaagttgag agccgtgggg ggatgacccg
     2401 taaggaatgg gctgactttg tggatcgaaa ttttaaccct cctaaataga gtaaattccg
     2461 attggcattg ttgcaggaat tatggtgccg cagcttattg ggtgtttgtc aattgccggg
     2521 aagtattcgc tggcatatca cttaaacggg agtagtaagt gagagaacct cgtccacgcc
     2581 atacattaca ggtagtctgt gtcccgtctc tgaatataca ggatctgggg ctaaccagtt
     2641 ttcatagctg gctcttatca aaaggctatg acacgaccaa tgccaggaac aaccgtactt
     2701 gctgggcaag ggaaggtggc tggcacctga agcgatgcag acatctggca acgggcgaag
     2761 atcatttctg gtttatcgca ttcaacggta ccggcggcga catttttcct gtgaaaaccc
     2821 agcaggacta ccgtgctgcc taccgcaagc ttgaagcgta tggatatgca ccggccgtta
     2881 ttgaacaatt gaacacaggc gttgcctata atcttgctta ttcacggaca ccgttaaaac
     2941 gccctgagag cgccaccagc gaactgaagc gaaaacctga cgtgaatatt cagggtgagc
     3001 cttgcgaacg ctttgtgacc gaacggagca gcgcagcgca gaataaattc aaaaccctgc
     3061 tcattgagaa cttcgccggt cggtgtgcag tcactggatg ggttaatggt ggtgtgctgg
     3121 tggcggctca tatcgagcat gggacgcgct acaacccttc gaacggcatc ctgttgacgc
     3181 cgacaatgca cgcattgttt gacgcggatc tgatgggtat agacccttca acactcaccg
     3241 ttcattttaa accgggcatc gaggcgggag agctgtttga aggaaaaacg ctcaatccgt
     3301 tggtgtatga tctggatacc gaccggctgg ctgctcgctg ggctgacttt ataggtgccg
     3361 agtgattgat gggaaattca gcctataacc gatggcagtg ttttcgggat ggacaccact
     3421 tactgatggg ggctggctgc acgccacctt tatattgaaa tccaacagag ttgatacaac
     3481 aaatgcaaaa ttgcctttcg ggatatttaa aagcgaccat tgttatttta tctgcggacc
     3541 tgacaggttg cgacctggca aatggggctg cggaaagtgg agtgtatcag aaatacacga
     3601 atggaccttt caaggaaccg actatcgtga tgagtccggg ctggcggatg gatgaccacg
     3661 gcagggaagc actaatattc gggagcagcc actgcccgga tgtaaacggc aataatacgt
     3721 ctgagggcgg ctgcgtcctc attgaggagc actctgcgac tgtcgctgtc actgttgttg
     3781 atgcgacaaa tcagtcccgt cgccaggaaa cctggacgat tgaacgtaaa aaggatcgag
     3841 ttatagtgaa gcgcccggat aacagttttg tgatgccctg gggaaaatag ccaagataca
     3901 agatgaccac aaaaatgaat aagccgcaat gttgttttgt gtgttgaacg tcctgatgga
     3961 gcctttgaca tttacactgg ccgttttata cagttccacc aaattgacaa cagtagaaga
     4021 aaaattgttg aaataagtga tcctgacctg gtggggatga cttattcaat cccgatggtt
     4081 tttaacggag ggaagttcag tggtcaaggg gttacctgtc tctcgctccc cgcagtaaca
     4141 ttaataaaag taacttttcc ttcccgaact tatcaggagg ggcaggaaca aatcacaatg
     4201 tcgagcccgg accaggcact attttctgaa gatgtccgga aaaactgcgc cggtacgtgt
     4261 gttcttaccg gcgttcgagg cagacaaaga actgaggctg cgcacataaa accccgtcat
     4321 gctggtggtg agccggatgt gacaaacggg attctactgc gaagtgacat ccataccctc
     4381 tttgacaatt ggcacttttc gatcgaccct gattcaatga aagcccgttt cagcccggat
     4441 gtgctcttcg tcgataaaga cttactacaa ctggaaggca agcaaattga tttttcccgc
     4501 ctgcaaatgc ctatcaatat tgaacacctt cgacatcact ggaacaaatt ttttaaccgt
     4561 catgtcaatc cattcataaa gaatgcgcta aataaaaaaa tcaacaatta tgaaaaacgg
     4621 ccagttaaca gcaacataca ttattaggct atgttagcct aatggcattc tgaaagtctg
     4681 cccaattaga ctgaagttca ataagaagga atgaaaatga acttgaaaag tattgttccg
     4741 aaggctgctg gccttgctgc atggattgta ggaatcattt atagtggttt tgctctttct
     4801 gttctgtggg ggtggttttt tgtgcctctc ggtgtggtgc cgatcacaac tccatgggct
     4861 attggaattt actgcttggc aggcataata agaggcaata gagtaaaaat cccgaaagaa
     4921 caagatatgc catgtacgct tcaattaacg atgatatatg tcggcatcac ctttttccta
     4981 attgtcggtt atatctgcca tcaaataact tagaactgat ttagaattaa atttgataaa
     5041 gcttactaca aagtagtttt gtcaatgttc aacctgttta tcaaacctac ggtgactttt
     5101 tatgactggg aatggtatca atactgtacg cataaacaac gaagtgaaac acatctcaga
     5161 gttagatccg gagatctctt tttcttgaat ggaataaact gaaaagtgaa aataacgaac
     5221 tgtatcgctg cataaaggaa gcaaattctg gttggcgtgg ttttgtcctt cgtttaatag
     5281 gggtttatct acctgatggc aaaacaattg tcattaaacc ctagattatt tgaatattcc
     5341 taaacatagg cttaataaga aaagccactg aaaagtggct tttgaactga gctgttcgcg
     5401 attttaattt caacttagat gaggaaatca tccagtgttt taccggcttc aagttgttcg
     5461 gccagtgctt taggggtacg cccctggcca gtccaggttt tcgtttcacc attaacatca
     5521 gtgtatttat atttcgcagg gcgcggttcg cgaacttttt taggtgctcc ggactttgca
     5581 gagaaagaac tcagtaactc ttcagggtca attccatctt caagcattaa ttgacgcagg
     5641 gattcaatct tctccagacg cgctttcagc tcagcttctt ttgaagattc ttcctgacgt
     5701 cgttcttcaa caacaacgct taatttttcc agcagttctt caaggatctc taacggcagt
     5761 tcacggccct gcgcacgaag agtacgaatg ttgtttaatg atttgagtgc ttcggacata
     5821 tgacctcact aagagtaata aataaaatca atagaatact ttgcgatatt acactctttt
     5881 attattttga gccagctaga cgcgtaagat tttctgggcg catgatatca atcattggta
     5941 cctaaagaca ttaaccaccc cgcgtatgga aacaaatgca ttatcacttg ccagttcctc
     6001 tgtagtgcca actccgcaag attagataaa atcgccgtct ttctcatgcg attatt
//
LOCUS       DAFNBJ010000232         5972 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000232 DAFNBJ010000000
VERSION     DAFNBJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.232"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            137..1240
                     /gene="ompC"
                     /locus_tag="L2X49_004584"
     CDS             137..1240
                     /gene="ompC"
                     /locus_tag="L2X49_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBN4799559.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQERGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1352..2407
                     /gene="apbE"
                     /locus_tag="L2X49_004585"
     CDS             1352..2407
                     /gene="apbE"
                     /locus_tag="L2X49_004585"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBN4799560.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2481..3545
                     /gene="ada"
                     /locus_tag="L2X49_004586"
     CDS             2481..3545
                     /gene="ada"
                     /locus_tag="L2X49_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBN4799561.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALHTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3545..4195
                     /gene="alkB"
                     /locus_tag="L2X49_004587"
     CDS             3545..4195
                     /gene="alkB"
                     /locus_tag="L2X49_004587"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBN4799562.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4271..5914
                     /gene="yojI"
                     /locus_tag="L2X49_004588"
     CDS             4271..5914
                     /gene="yojI"
                     /locus_tag="L2X49_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBN4799563.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1417 a         1507 c         1629 g         1419 t
ORIGIN      
        1 aagttttagt atcatattcg tgttggatta ttctgcattt ttggggagaa tggacttgcc
       61 gactgattaa tgagggttaa tcagtatgca gtggcataaa aaagcaaata aaggcatata
      121 acagagggtt aataacatga aagttaaagt actgtccctc ctggtcccag ctctgctggt
      181 agcaggcgca gcaaacgctg ctgaagttta caacaaagac ggcaacaaat tagatctgta
      241 cggtaaagta gacggcctgc actatttctc tgacaacaaa gatgtagatg gcgaccagac
      301 ctacatgcgt cttggcttca aaggtgaaac tcaggttact gaccagctga ccggttacgg
      361 ccagtgggaa tatcagattc agggcaacag cgctgaaaac gaaaacaact cctggacccg
      421 tgtggcattc gcaggtctga aattccagga tgtaggttct ttcgactacg gtcgtaacta
      481 cggcgttgtt tacgacgtaa cttcctggac cgacgtactg ccagaattcg gtggcgacac
      541 ctacggttct gacaacttca tgcaggagcg tggtaacggc ttcgcgacct accgtaacac
      601 tgacttcttc ggtctggttg acggcctgaa ctttgctgtt cagtaccagg gtaaaaacgg
      661 tagcgtaagc ggcgaaggca tgaccaacaa cggtcgtggt gctctgcgtc agaacggcga
      721 cggcgttggc ggatctatca cttatgatta cgaaggcttc ggtatcggtg gtgcgatctc
      781 cagctccaaa cgtactgatg atcagaacag cccgctgtac atcggtaacg gcgaccgtgc
      841 tgaaacctac accggtggtc tgaaatacga cgctaacaac atctacctgg ctgctcagta
      901 cacccagacc tataacgcaa ctcgcgtagg ttccctgggt tgggcgaaca aagcacagaa
      961 ctttgaagct gttgctcagt accagttcga cttcggtctg cgtccgtctg tagcatacct
     1021 gcagtctaaa ggtaaaaacc tgggtaccat cgctggtcgt aactacgacg acgaagatat
     1081 cctgaaatat gttgatgttg gtgctaccta ctacttcaac aaaaacatgt ccacctacgt
     1141 tgactacaaa atcaacctgc tggacgacaa ccagttcact cgtgacgctg gcatcaacac
     1201 tgataacatc gtagctctgg gtctggttta ccagttctaa tctcgattga tatcgaacaa
     1261 agggcctgcg ggcccttttt tcattgtttt cagcgtacaa acacagtttt ttggtgtact
     1321 cttgcgaccg ttcgcatgag gataatcacg tatggaaata agctttaccc gcgtggcact
     1381 gctggctgcc gcgctctttt ttgttggttg cgatcaaaaa ccacaacccg ccaaaaccca
     1441 cgctactgaa gttaccgttc ttgaaggcaa aaccatgggt accttctggc gtgccagcat
     1501 cccgggcatt gatgccaaac gcagtgccga acttaaagaa aagattcaga cccagctgga
     1561 cgccgacgat cagctgcttt cgacctataa aaaagattcc gcgctgatgc gctttaacga
     1621 ttcgcaaagt ttatcgccgt ggccggtaag tgaagcgatg gccgatatcg tcaccacctc
     1681 gctgcgcatt ggcgcgaaga ccgatggcgc gatggatata accgtcgggc cgctggtgaa
     1741 tctgtggggc tttggcccgg aacaacagcc ggttcaaatt ccgagccagg aacagatcga
     1801 tgcgatgaaa gccaaaaccg gcttacagca cctgacggtc attaatcagt cgcatcagca
     1861 atatctgcaa aaagacctgc cggatttaaa tgtcgatctc tctaccgtcg gcgaaggtta
     1921 tgcggcggat cacttggcac gcttgatgga gcaggaaggg atttcccgct atctggtgtc
     1981 ggtgggcggc gcgctgaaca gccgtggtat gaacggtgaa ggccagccgt ggcgggtagc
     2041 gattcaaaaa ccaaccgata aagaaaacgc ggttcaggct gtggtggata tcaacggtca
     2101 tgggattagc acctctggca gctaccgtaa ctattacgaa ctggacggca aacgtctttc
     2161 ccatgttatc gatccgcaaa ccgggcgtcc catcgaacac aatctggtat ccgtgacggt
     2221 gattgctccg acggcgctgg aagccgatgc ctgggatact ggcttgatgg tactcgggcc
     2281 ggagaaagcc aaagaggttg ttcgccggga agggctggcg gtttatatga tcaccaaaga
     2341 aggcgatagc tttaaaacct ggatgtcacc acagtttaaa agcttccttg tcagcgaaaa
     2401 aaattaaagc gcaagattgt tggtttttgc gtgatggtga ccgggcagac taaaggctat
     2461 ccttaaccag ggagctgatt atgaaaaaag ccacatgctt aactgacgat caacgctggc
     2521 aatctgtctt agcccgcgac ccgaatgccg acggcgaatt cgttttcgcc gtgcgtacca
     2581 caggcatctt ttgccgtccg tcttgccgcg ccagacatgc tttgcgggaa aacgtctcct
     2641 tctacgcaaa tgccagcgag gcactcgccg ctggctttcg cccctgcaaa cgttgtcagc
     2701 cagaaaaagc caatgcccag caacatcggt tggataaaat cacccacgcg tgtcgactgc
     2761 tggaacagga aacgcctgta acgttggaag ccttagccga ccaggtggcg atgagtccat
     2821 ttcatctaca tcggttgttt aaagcgacta ccggaatgac gcctaaagcc tggcaacagg
     2881 cctggcgcgc tcgccgtttg cgcgaatcgc tggcgaaagg ggagagcgtg acgacgtcta
     2941 ttcttaacgc cggattcccc gacagcagca gttactatcg caaagctgac gaaacgctgg
     3001 gcatgacggc taaacaattc cgtcacggtg gcgaaaatct ggcggtgcgt tacgcgctgg
     3061 ctgattgtga gctgggtcgt tgcctggtgg cagaaagcga gcgggggatt tgcgcgatat
     3121 tgctgggcga tgatgacgcg acactaatca gcgagttgca gcagatgttt cccgctgccg
     3181 acaacgcgcc tgccgatctg atgtttcagc aacatgtgcg tgaagtgatc gccagcctca
     3241 atcaacgcga tacgccgctg acgttaccgc tggacattcg cggcactgct tttcagcaac
     3301 aagtctggca ggcactgcac acgatacctt gcggtgaaac cgtcagttat cagcaactgg
     3361 ctaacgccat cggcaaaccg aaagcggtac gggccgttgc cagcgcctgt gccgccaaca
     3421 agctggctat cataataccc tgtcatcggg tggtccgtgg tgatggcaca ctttccggtt
     3481 accgctgggg cgtgtcgcgt aaagcgcaac tgctgcgccg cgaagctgaa aatgaggaga
     3541 ggtaatgttg gatctgtttg ccgatgctga accgtggcaa gagccactgg cggctggagc
     3601 ggtaattttg caccgttttg cttttaacgc tgcggagcaa ctaatccgcg atattaatga
     3661 cgttgccagc cagtcgccgt ttcgccagat ggtcaccccc gggggatata ccatgtcggt
     3721 ggcgatgacc aactgtgggc atctgggctg gacgagccat cggcaaggtt atctctattc
     3781 gcccattgat ccgcaaacaa ataaaccgtg gcccgccatg ccacagagtt ttcataattt
     3841 atgtcaacgt gcggctacgg cggcgggcta tccagatttc cagccagatg cttgtcttat
     3901 caaccgctac gctcctggcg cgaaactgtc gctgcatcag gataaagacg aaccggatct
     3961 gcgcgcgcca attgtttctg tttctctggg cttacccgcg atttttcaat ttggtggcct
     4021 gaaacgtaat gatccgctca aacgtttgtt gttggaacat ggcgatgtgg tggtatgggg
     4081 cggtgaatcg cggctgtttt atcacggtat tcaaccgttg aaagcggggt ttcatccact
     4141 caccaccgac tgccgctaca acctgacatt ccgtcaggca ggtaaaaaag aataaaaata
     4201 agaattatta ttgctgtgcg cgcgaagatg tttaaactgc gggctgtaat tcattgtccg
     4261 ggttttctgc atggaacttc ttgtacttgt ctggcggcag tatcgctggc catttatcag
     4321 tgtgatggcg ctaagcctcg ccagtgcggc attaggcatt ggcttaattg cttttatcaa
     4381 tcagcgcctt atcgaaacgg cggataccag tctgctggtg ttgccggagt ttcttggatt
     4441 attactgctg ttgatggcag tcactctcgg atcgcaactg gcgctcacca ctttggggca
     4501 tcacttcgtt taccgactgc gtagtgaatt tatcaagcgg attctggata cccacgtcga
     4561 gcgcattgaa caactcggta gcgcctcgtt gctggcggga ttaaccagcg atgtgcgcaa
     4621 tatcaccatt gcttttgtgc gtcttccgga actggtgcag gggatcattc tcactatcgg
     4681 ttccgcggcg tatctgtgga tgctgtcggg caaaatgttg ctggtaacgg ctatctggat
     4741 ggcgatcacc atctggggtg gttttgtact ggtggcgcgg gtgtacaaac atatggcgac
     4801 cctgcgtgaa accgaagaca agctgtacac ggattttcaa accgtactcg aagggcgcaa
     4861 agagctgact ctgaaccggg aacgcgccga gtatgtgttt aacaacctct acatccccga
     4921 tgcgcaagag tatcgccatc atattattcg cgcagacacc ttccatctta gcgccgtgaa
     4981 ctggtcaaac atcatgatgc tgggcgctat cggcctggtg ttctggatgg cgaacagtct
     5041 cggttgggct gataccaacg ttgctgcgac ctattcgttg acgctgttat tcctgcgtac
     5101 gccgctgctt tcggcggttg gcgcattgcc gacgctgctg acggcgcagg tggcgtttaa
     5161 caagctgaac aaattcgcgc tcgcgccttt caaagctgag tttccgcgcc cgcaggcttt
     5221 tcccaactgg caaacgctgg agctgcgtaa cgtgacgttt agctatcagg ataacgcgtt
     5281 ttccgttggg ccgattaacc tcaccattaa acgtggcgag ctgctgtttc tgattggtgg
     5341 caacggtagt ggaaaatcga cgctggcgat gttgctgacg ggcttgtacc agccacaaag
     5401 cggcgaaatc ttgctggatg gcaaacctgt cagtggcgaa caaccggaag attatcgcaa
     5461 actgttttcg gcagtgttta ccgatgtctg gctgtttgat caactgctgg ggccggaggg
     5521 taaacccgct aacccgcaac tggttgagaa gtggctggcg cagctgaaaa tggctcataa
     5581 gcttgagtta agcaacgggc gtattgttaa cctgaagtta tcaaaagggc agaaaaaacg
     5641 cgtggcgctg ttgctggcgc tggcagaaga acgcgatatt atcctgctgg atgaatgggc
     5701 ggcggatcag gatccacact tccgtcgtga gttttatcag gtgttgctgc cgctgatgca
     5761 ggagatgggt aaaactattt tcgctatcag tcatgatgat cattacttta tccacgccga
     5821 ccgcctgctg gaaatgcgca atgggcaact tagcgagctg acgggcgaag agcgcgatgc
     5881 cgcttcgcgt gatgccgttg cccggacggc ataatattac gacgctgacc tggcggactg
     5941 ctgccgtcag gtcaatatgc taaaaatccc tt
//
LOCUS       DAFNBJ010000233         5954 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000233 DAFNBJ010000000
VERSION     DAFNBJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.233"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            157..1362
                     /gene="chrA"
                     /locus_tag="L2X49_004589"
     CDS             157..1362
                     /gene="chrA"
                     /locus_tag="L2X49_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HBN4799564.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            1373..1678
                     /locus_tag="L2X49_004590"
     CDS             1373..1678
                     /locus_tag="L2X49_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4799565.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            1722..1787
                     /locus_tag="L2X49_004591"
                     /pseudo
     CDS             1722..1787
                     /locus_tag="L2X49_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            1905..2669
                     /locus_tag="L2X49_004592"
     CDS             1905..2669
                     /locus_tag="L2X49_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HBN4799566.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(3162..3746)
                     /locus_tag="L2X49_004593"
     CDS             complement(3162..3746)
                     /locus_tag="L2X49_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN4799567.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3746..4984)
                     /locus_tag="L2X49_004594"
     CDS             complement(3746..4984)
                     /locus_tag="L2X49_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4799568.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(4981..5886)
                     /locus_tag="L2X49_004595"
     CDS             complement(4981..5886)
                     /locus_tag="L2X49_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBN4799569.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         1195 a         1769 c         1822 g         1168 t
ORIGIN      
        1 aagcacgcta agccgtaagt aagcgtgctc ctgtgaaagc cacagctaaa actgcgtagt
       61 acacatagag gtattttttt ctagatttat atcgaatttc gttatcgaat ttcgcttatt
      121 attgatcaac tcgttgcaga tcatgaaggc gaaagtatga cgaataaccc taccgatgac
      181 agcagaccat ggtcggtctt tcttattttt ctgcggcttg gattgacatc ttttggcggc
      241 cccattgcgc acttgggcta cttccgcgcc gaatttgtca cacggcggcg ctggctctcc
      301 gaacggagct atgctgactt ggtcgcgctt tgtcagttct tgccagggcc tgcaagcagc
      361 caggtcggca tagcggtagg actgtctcgg gctggataca gcggggcgct ggctgcttgg
      421 gctggcttca cgctgccgtc tgccatagcc ttgatccttt ttgcgctcgg catctccagc
      481 tatggcgatt acgtctcgca gggcgcgttg catggcttaa aagtggtggc tgtggccgtg
      541 gtcgctcaag cagtatgggg catggcgcgt aacctatgca cggatgggct gcgagtcacc
      601 atcatggcaa ttgctacctg cgtcgtttta cttgtgccgt ccgcgtgggg acaggttggc
      661 gtgattgcta tcgcaggcat cgcaggccgg ttattgttca agccagcgaa agttgttgag
      721 catgaccccc tacctatcac ggtcagtcac cgggccggcg tgctttggct ctcgctgttc
      781 tttgtcttgc tgattggcct gccggtgttg gccgaactga tgccaagtca aaccatggca
      841 atggtggatt ccttctatcg tgtcggatca ctggtgttcg gcggtggtca cgttgtgctg
      901 ccattactgc aagccgaagt ggtgccctcc ggctgggtca acaatgaatc ctttctcgcg
      961 gggtacgggg cagctcaagc ggtgcccggc cctttgttca cgttcgccgc gtttcttggt
     1021 gcctcgatga acaccgcccc gtcgggctgg atcggcggca ttgtgtgtct gctggctatc
     1081 ttcgcgccct cgttcttgct ggtcgtcgga tcaatgccat tttgggagcg tttgcgccgc
     1141 aatacaggca tccaagctgc gctggccggg atcaatgccg ctgtagtcgg cttgctgctg
     1201 gccgcgctgt atcagcctgt atggactagc gccatctttc agccgcaaga cttcggcttg
     1261 gcattagttg cccttgtcgc acttatgttc tggaagctcc cgccgtggct ggttgtcctc
     1321 ggtagcggtg cagctgggtg gctgttgagc gtcgctctgt gaggcacaaa aaatgactga
     1381 caaagacctc tacggcggtt tgatccgcct gcacatcctt caccatgcag ccgaggaacc
     1441 tgtctttggg ctggggatca tcgaagagct acgccgacac ggctacgaga tgagcgctgg
     1501 caccgtgtac ccgatgctgc acggcctgga aaaaaaaggc tatctgacct cacgccacga
     1561 acgcaccggg cgacgtgaac ggcgtgttta tgacatcact gaacaaggca gaactgcact
     1621 tgctgatgcg aaaacaaagg tcaaggagct gttcggagag ttggtagaag gtggttgagt
     1681 ttttgcttct atgtcaggtt gagctatacc ctaactggat tgtcattttc agaagacgac
     1741 tgcaccagtt gattgggcgt aatggctgtt gtgcagccag ctcctgacag ttcaatatca
     1801 gaagtgatct gcaccaatct cgactatgct caatactcgt gtgggctctg ttgcaaaaat
     1861 cgtgaagctt gagcatgctt ggcggagatt ggacggacgg aacgatgacg gatttcaagt
     1921 ggcgccattt ccagggtgat gtgatcctgt gggcggtgcg ctggtattgt cgctatccga
     1981 tcagctatcg cgaccttgag gaaatgctgg cggaacgcgg catttcggtc gaccatacga
     2041 cgatctatcg ctgggtccag tgctacgccc cggagatgga gaagcggctg cgctggttct
     2101 ggcggcgtgg ctttgatccg agctggcgcc tggatgaaac ctacgtcaag gtgcggggca
     2161 agtggaccta cctgtaccgg gcagtcgaca agcggggcga cacgatcgat ttctacctgt
     2221 cgccgacccg cagcgccaag gcagcgaagc ggttcctggg caaggccctg cgaggcctga
     2281 agcactggga aaagcctgcc acgctcaata ccgacaaagc gccgagctat ggtgcagcga
     2341 tcaccgaatt gaagcgcgaa ggaaagctgg accgggagac ggcccaccgg caggtgaagt
     2401 atctcaataa cgtgatcgag gccgatcacg gaaagctcaa gatactgatc aagccggtgc
     2461 gcggtttcaa atcgatcccc acggcctatg ccacgatcaa gggattcgaa gtcatgcgag
     2521 ccctgcgcaa aggacaggct cgcccctggt gcctgcagcc cggcatcagg ggcgaggtgc
     2581 gccttgtgga gagagctttt ggcattgggc cctcggcgct gacggaggcc atgggcatgc
     2641 tcaaccacca tttcgcagca gccgcctgat cggcgcagag cgacagccta cctctgactg
     2701 ccgccaatct ttgcaacaga gcccgccgtg ctagtctgct cggtgatggt ggagtgaagc
     2761 caacccgcaa tcgggttatg aatctgcatc gcgattcgca atcagctgtc tcttgagcat
     2821 gtcgaactcc tgcgatgtta tctcgccgtg ctcgtgctgc tacaccaact tgtccaactc
     2881 atccgccaca ccgctcccca ccacctgcga tggccaggtt gtttgctggg cgcattggcg
     2941 gagtatcgtc tcgacccggg acacccacac cggcacaacc ttacgggagt gattcactgt
     3001 caaagaatcg gcccggtgct ctgacgcaag tatcgggatg gtcaccattt gtaagccgta
     3061 gacctgagtg gtgatcaaga cttcgatacc accgaccgta ccggtactaa tcgacgacgg
     3121 tcgtgttcgt cgcctgccgc agggactctg cacacctccg tttacgcatg tgcctggagg
     3181 agttggaaat cgtcgtgttc gggaaacatt aaacacagga tggcagcgat ctgagccagc
     3241 acatgatcag ctagctcacc atccggatcg acggcccact gcatcgtcgc gccagcgatg
     3301 accgagtgca ggagcaactc agctgccgca ggagcacctg ggggcagtcg cttgcggatc
     3361 ccctccacca ccgcgcggtt ccgctggatc gcaagcgtgc gtagctccgg cacctggagc
     3421 tcgtaccagg agatgagata gttcaccgag aagtcgttgc gagtgttcat gctccgaacg
     3481 agcacctgca aaaattccca gagcccttgc ggccctgcgc ctatcggtat cgcattcagg
     3541 taatgccgca cctgctcgac gccgcgctcc atcatcctca ccagcagcgt atcgcggttg
     3601 gtgaagcgct ggattaacgc tgcgcgggag agccccacct cctttgctac tccgctgagc
     3661 gtgaactcta tgggaccgca acgcttcagc actacggtgg cggcctcgag tacctcgtca
     3721 tcggacttga gcttggggcg gggcatcagt gttcaccttc tgtatgggtt ggggcggagg
     3781 ctgtggctgc cgccgccatt gtagcaaatt gaagacggag cgagagtaga gccacgagcc
     3841 ccgcaaacac ggccgataca acgaggccag ggagcggacc agcaaggtcg acaaacgcgc
     3901 cggccgcaag cataaccatg ggcgaggctg acagcatcac cgccgagacc gtgccgagta
     3961 cccggccgag aagttctggc ggcgtgcggt tgtagatggc agcgttgaga atgggagaga
     4021 ctgagccggt cagcagtccc acgagcgcgc ccaacaacat cagcaccggc acgcctggca
     4081 actgtgaaag cagaagcgag cccaccgcag agccacaaaa tgccaccgcc agccagttct
     4141 gcgctgatat ccgggcgccg accgacgcat gaatggcaat gccaaggaga ccaccagccc
     4201 ccatcattga ggagaacagc ccgagctctg ctacttggcg tcctgcatct acaaacagcg
     4261 caggcatgat gacgctgccg ttggcgccaa cgatgcccac gaagatcatc actataccaa
     4321 agagagggcg cagcaggggt tcgctccaga gaaaagcgac gccggcgcgc atggagagag
     4381 tcgccgtcgt ggtcatcgtc cgagcggcac gcgcgggaag cacccacgcg ccgagcagac
     4441 ctgcaaggac ggagcagaac gccgtcagcc cgagcgttgg cgcagcgcca agcaggccga
     4501 ttgcggcccc cccaagggcc gggccaccta gaatcgcgac gttcccgatc accgctttca
     4561 gtgacgagac gcgctcaacg gagagcccgg cgacgtggcc gagtttgggc agctcactgt
     4621 cctgcgcggc cataccgggt gcgtcgaacg cggcaccgag caccacgcaa gcgatcagcc
     4681 cagtgttcga gagggcgcca acggcatcga gcagtgggat gctcgccatg gccacgccgc
     4741 ccaccacacc cgagatcaat gcgacgggcg cgcgcccgaa ccgatcgacg aggccaccac
     4801 caacccacgc gccgatgatg gtcgcgatga cgctgctagc ggccgtggcg cccgcccagg
     4861 ccgcgctctt tgtatgagac aggacgaacc atggaagcgc gagggccgcc accgcgttgc
     4921 cgatccggaa gagaaaggtc gccgcgaaca gcgtcgcgag cgggctatat cgacgttcgc
     4981 tcattccgct gcggcgagct gcgccttcgc cgcagcgagg tactcttcgt tacccgagtc
     5041 gagggcgaag agtgcgtagg tgaccgcccc gaacgcaagg cgctccgcga tgtggtgggc
     5101 gagccgcggc cacacccggc caccggccgc ttcatacgtg aggaggagct tcgcgagccc
     5161 ctcttcacca aagaccataa ggtgcgcggc catgtcgatg gcagggtcat caacgcgggc
     5221 ctcgctccag tcgatcatcc cgctgacgcg ctccgtgttg tcgatgagca catggcccac
     5281 gtagagatcg ccatgcacca ccacggagaa atctggccac gacgaatcgt cgtcgagcca
     5341 gcgctgccac cggtggaggc gcttgtcgtt caccacgaac tcgcgtcgga cgcggtcaac
     5401 gtcgtcggcc accttctgac gggcctgcgt cggtgtacgg atgagcatcc ccgcatccac
     5461 ggcggcggaa atggggacgg catgcagggc ggcgagcgcg gtcgcgaagc tctccgcgaa
     5521 gacctccgag tcctgcggca cgacccagtc gggcgtggac gaaccaggct ggatgaccat
     5581 cgcagtcgag tcttcgagca tgggataggc aacgagctcg gcgttggcca cgcgccagtc
     5641 cggcaccgcg aacggcaggc gattcttgag cattgccagc acccgcgcct ctggttcgac
     5701 cttcgcgctt acctcggctc ggcgcgggat gcgcagcacc caccgacgtc catcgtcgac
     5761 ggtggcgatc acgatcctat agtcgagccc aagctcattg acagtcagcg ggccatggag
     5821 cttgagccca tgtcgggctg caagtgcgta cagttgggag gtatcggcgg tcgtgactac
     5881 ggtcatgatt cactcctgag ggcttgacgg gtttagccac ctaaatgtaa cagtcacgtc
     5941 ggttatattc aatc
//
LOCUS       DAFNBJ010000234         5950 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000234 DAFNBJ010000000
VERSION     DAFNBJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.234"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            62..295
                     /locus_tag="L2X49_004596"
     CDS             62..295
                     /locus_tag="L2X49_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBN4799570.1"
                     /translation="MNKSDEMIQELPDGAFTRECAETVVAQYRNVFIEDDQGTHFRLV
                     VRQDGSLIWRSWNFEDCAGYWMNRYIRDFGIRK"
     gene            357..2315
                     /locus_tag="L2X49_004597"
     CDS             357..2315
                     /locus_tag="L2X49_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBN4799571.1"
                     /translation="MSVVKSEPDTTRKASRKSAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVDGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAED
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEIRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKAPVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQMDATEHTTNLADAA"
     gene            2370..2804
                     /gene="psiB"
                     /locus_tag="L2X49_004598"
     CDS             2370..2804
                     /gene="psiB"
                     /locus_tag="L2X49_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBN4799572.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNDGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            2801..3520
                     /locus_tag="L2X49_004599"
     CDS             2801..3520
                     /locus_tag="L2X49_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBN4799573.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            3650..4825
                     /locus_tag="L2X49_004600"
     CDS             3650..4825
                     /locus_tag="L2X49_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBN4799574.1"
                     /translation="MLRATKIRIYPTPEQAAFLNAQFGAVRFAYNKALHIKKHAYQRH
                     GVNLSPRKDLKPLLSVAKKSRRYAWLKEFDSMALQQAVINLDVAFSNFFNPKLKARFP
                     TFKRKHGKQSSYHCVGIKVQDNAIKIPKLAPIEARLHRELHGKLKSITVTRSATGKYY
                     ASILCDDGASAPEKPTYLKEEKITGLDMGLEHYAITSDAVKVPNPRHLINAGRNLRRK
                     QKALSRKQKGSANRKKARIRLAALHERVANARADFQHKLSRAIVDENQAVIVETLKTA
                     NMMKNHNLARAIGDAGWHSFITKLEYKTATKGVHLVKLDQWFASSKTCHCCGYKMPEM
                     PLHKRIWRCHECGAEHDRDINAALNIRQKGILELKAAGLVVSAHGGQRKSVAQTVAA"
     gene            5273..5773
                     /locus_tag="L2X49_004601"
     CDS             5273..5773
                     /locus_tag="L2X49_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBN4799575.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT         1343 a         1571 c         1771 g         1265 t
ORIGIN      
        1 tctgatctgg ctgatttttc tgacagcccc accatgtgtg gggcattacc ggagaacgat
       61 gatgaacaaa tccgatgaaa tgattcagga actacccgat ggggcattca cccgcgagtg
      121 tgctgaaacc gttgttgcac agtaccggaa tgtctttatc gaggatgatc agggaacgca
      181 ttttcgcctg gttgtccgtc aggatggctc gttgatctgg cgctcctgga attttgagga
      241 ctgtgccggg tactggatga accggtacat cagggatttc ggcattcgta agtaagagag
      301 gtgccggacg gggatgacgt ccggcagaca ttaactaaac cgtaagggga ataatcatgt
      361 cagtagtaaa gtctgaacca gacaccaccc gcaaagcaag ccgtaaatct gcaaaaacgc
      421 aggaaacagt cctgtctgcc ctgctggcgc agacggagga agtgagcgtg ccgctggcct
      481 cgctgattaa gtcaccactg aatgtgcgca cggtgccgta ttctgcggag tccgtcagcg
      541 aactggctga gtccattaag ggcgttggcc tgctgcagaa tctggtcgtg catgccctgc
      601 ctggtgaccg ccacggtgtc gccgcaggtg gtcgcagact ggcagcactc aatatgctgg
      661 cagagcgtgg catcattccg gctgactggc ctgtccgcgt gaaaattatt ccgcaggaac
      721 tggcgacggc tgcatcgatg accgagaacg gtcatcgtcg ggatatgcac ccagccgaac
      781 agattgccgg attccgcgca atggcgcagg aaggcaaaac acctgcgcag actggcgacc
      841 tgctgggcta ttcaccccgc cacgtccagc gaatgctgaa actggcagac cttgcacccg
      901 tcatccttga tgcgctggca gaagaccgca tcaccaccga acactgtcag gcgctggcgc
      961 tggagaacga caccgcgcgt caggtgcagg tgtttgaagc cgcctgccag tcaggatggg
     1021 gcggtaaacc ggatgtgcgg gttatccgca acctgattac cgaaagtgaa gtggcggtgg
     1081 acgggaacag taaattccgc ttcgtggggg ctgatgcctt ctcgccagac gaactgcgca
     1141 ccgatttgtt cagcgatgac ggggacggtt atgtcgaccg cgtggcgctc gatgccgccc
     1201 tgctggaaaa actccaggct gtcgctgaac accttcggga agccgaaggc tgggaatggt
     1261 gcgccgggcg catggagcct gtcggtgagt gccgtgagga tgccggaaca taccgctgtc
     1321 tgccggagcc ggaagcggtg ctgacggagg cggaagacga acgcctgaac gaactgatga
     1381 cgcgttacga cgcgctggaa aaccagtgtg aggaatccga cctgctggaa gcagaaatga
     1441 agctgatgcg ctgcatggcg aaggtcagag cgtggacgcc ggagatacgt gccggaagcg
     1501 gtgtggtggt gtcctggcgt tatggcaacg tatgtgtcca gcgtggtgtg cagttgcgca
     1561 gtgaagatga cgcggctgac gacgctgacc gcacggaaca ggtgcaggag aaagcgcccg
     1621 tggaggaaat cagtctgcca ctgctgacga aaatgtcttc cgaacgcacg ctggcagtcc
     1681 aggcggcact catgcagcaa ccggacaaat ctctggcact gctggcatgg acgctctgcc
     1741 tgaatgtgtt tggcagcgga gcgtacagta aaccagcaca aatcagcctg gaatgtaaac
     1801 attattcgct gaccagcgat gcgccgtcag ggaaggaagg tgccgcgttc atggcgctga
     1861 tggcagaaaa agcccgtctt gctgccctgt taccggaggg atggtcacgg gacatgacga
     1921 cgttcctgtc gctcagccag gaggtgctgt tatccctgct cagtttctgc accgcgtgca
     1981 gcatccacgg cgttcagacc cgcgagtgtg gtcacacgtc acgcagcccg ctggacacgc
     2041 tggagagcgc catcggtttt cacatgcgcg actggtggca gccgacaaaa gcaaacttct
     2101 tcggacacct gaaaaagccg cagattatcg cagccctgaa tgaggcagga ctgtccggtg
     2161 ccgcacggga cgcggagaag atgaagaaag gcgatgcggc tgaacatgca gagttccata
     2221 tgaaagacaa ccgctgggtg cctggctgga tgtgtgcacc acgtccacag atggatgcca
     2281 ctgaacacac cactaacctg gctgatgccg cctgatgaac aaccacaccg ccccgccgga
     2341 gacggggcgg cagcaaggga gataccgtga tgaaaactga actgaccctg aatgccctgc
     2401 agtccatgaa cgcacaggaa tatgaagata tccgtgctgc gggaagcgat atgcgccgta
     2461 atctcactca cgaggtgatg cgtgaagtgg acgcaccggc taactggatg atgaatggcg
     2521 agtatggcag tgagttcggg ggctttttcc ccgtccaggt tcgtttcacg ccagcccacg
     2581 aacgtttcca cctggcatta tgttcgccgg gagacgtctc tcaactctgg atgctggttc
     2641 tggtgaatga tggtggacag cctttcgccg tcgttcaggt gcaacatatc ttcacgcctg
     2701 tcgcgatcag tcacacgctg gcgctcgccg cgacacttga tgcgcaggga tacagtgtta
     2761 acgacatcat tcatattttg atggcagaag gaggtcaggc atgagcgcac gttcacgggc
     2821 actgatcccc ctcagcgcag aacaacaggc cgccatgcag gctgtggctg tcaccgaaca
     2881 acgtcgtcgt cagggacgca cactttcagc atggccttat gccagcgctt tctttcgctg
     2941 cctgaatggc agtcgccgga tttcgctgac cgatctccgc ttttttgctc ctgcgctgac
     3001 gaaggaggaa tttcatggca accgtctcct gtggctggct gccgtggata aactgattga
     3061 aagttttggt gaagtctgtg ttcttcccct gccatccgat gcggggcatc gtctgttccc
     3121 gtccgttcct tttcgtgaag gtgagcggcg tcgtcagaaa accacgctga cagagcagaa
     3181 atacagccgc cagcgggaac gtgaggcaga acgacgggaa ctggaatacc agacatgttt
     3241 tgctcaggcg cagattgacc ttgcgtttca tactcccgcc acggtcggaa gctggttgtc
     3301 acgctggtct ggtgttgttg aggagcatga tctggaaacg attttctggg ggtggtgcgg
     3361 gcgttttcca tcactgtcat catttgaccg gtttttctgg caggaggaac cactctggcg
     3421 gctgattttt gaagccggtg aggccggtcg tggtgcaccg gtacaggtac gtgcacttga
     3481 gcagtggatg atcccgaaca agctggagaa cgcaatatga tgaaatcagc gaaaagccgc
     3541 cgagaaccct cgcccaatgc gggtggggag gaaaggcgga aaagcccgaa gggctttaaa
     3601 aatattatta cggtgttagc ataaaaacct gtaccaatga gcggtgacta tgttaagagc
     3661 aacaaaaata cgcatctatc ctactcctga acaggctgca tttctcaacg cccagttcgg
     3721 tgcggtacgt tttgcgtaca acaaagctct tcacatcaaa aaacacgctt accagcgaca
     3781 cggagtgaat ttaagtccgc gtaaagattt aaaaccgttg ttgtctgtgg cgaagaagtc
     3841 ccgcagatat gcctggctca aagaatttga ctcaatggca ttgcaacagg cggtaatcaa
     3901 tcttgatgtg gcattttcca acttttttaa tccgaagctg aaagcccggt tcccgacgtt
     3961 taaacgcaaa cacggaaaac aatcgagcta tcactgtgtc gggattaagg tgcaggataa
     4021 tgccatcaaa atccccaaac tggccccgat agaagcacgc cttcatcgtg aacttcatgg
     4081 aaagctgaaa agcatcactg tcacccgttc tgcaacgggg aaatactacg cttccattct
     4141 ttgtgacgat ggcgcttccg caccagagaa acccacgtac ctgaaggaag aaaagattac
     4201 aggtctggat atgggactgg agcactatgc catcacttct gatgctgtaa aagttccgaa
     4261 tcctcgccat ctcatcaatg caggtcgtaa tctgcgacgc aaacaaaaag cattatcccg
     4321 taagcaaaag ggaagcgcga atcgtaaaaa ggccagaatc cgtcttgcgg ccttacacga
     4381 acgggtggcg aatgcccgtg ctgattttca acacaagctc tctcgtgcaa ttgttgacga
     4441 aaaccaagcg gtaattgtgg agacgctgaa aacagccaac atgatgaaaa accacaatct
     4501 ggcaagagcg ataggtgatg caggctggca cagcttcatc acaaagctgg agtacaagac
     4561 agcgacaaaa ggcgttcacc tggtaaaact cgaccagtgg tttgccagtt cgaaaacgtg
     4621 ccattgttgt ggttacaaaa tgccggaaat gccacttcat aaacgcatct ggcgatgcca
     4681 tgagtgtgga gcagagcacg accgcgatat caatgcagca ctcaacatcc ggcaaaaagg
     4741 aatactggaa ctaaaggcgg cgggactcgt cgtctctgcc catggaggcc agcgtaaatc
     4801 cgtcgcacag acggttgcgg cctgagaagt gggaagcctc gcccgacagg gcggggagca
     4861 gtcacgggtg gtctggaggt catgcggcgt gtcctctgca ctcgccggaa taaggaagtc
     4921 gccggcggct ccgcttttac ccggccatgc ggggcatggc cttgtgggtt ttcagctatg
     4981 tggcctcagc atcgtgtgcg ggctgtgccg tgcctccatc gtagccgggc tggcgttgat
     5041 gcaagggtac gcttcgccgc tgcggtcacc cggtccctcc ttcccgtctg ccgtgatttt
     5101 ccggtcgttc gcccgctcag gtttcgcggc ctcaccctgc aactcccgcc agccgtgtgc
     5161 gtcaggctgc ggcttccctt gcgccctgtg catccccgcc ttatcgcccg gcttttatgg
     5221 aggcacggca ccgcccggtg ccgcagaaca tgtgactatg gaggattcgg gaatgtctgt
     5281 tgttgcacct gctgtatacg ttggaacctg gcacaaatac aactgtggaa gcatcgccgg
     5341 acgctggttt gacctgacca cgtttgatga tgagcgcgac tttttcgccg cctgccgtgc
     5401 tcttcaccag gatgaagccg atcctgaact gatgtttcag gattatgagg gattcccggg
     5461 gaatatggcc tctgaatgcc atatcaactg ggcctgggtt gaaggcttcc gccaggcacg
     5521 ggatgaaggc tgcgaagagg cttatcgtct ctgggtggag gataccggtg agacggattt
     5581 tgacaccttc cgcgatgcct ggtggggcga ggctgacagt gaggaggctt ttgcggttga
     5641 gttcgccagt gataccggcc tgctggctga cgtgccggag acggtggcgc tctattttga
     5701 ctatgaggcg tatgcgcggg atttattcct ggactccttc acctttattg acggtcatgt
     5761 gttccgtcgg tgatttactc ccccgctccg gcgggggtat ggcctgccag gccgccggaa
     5821 aaccctggca ggcttacgcc tgcctgaaat gcaggagggc cgcgcctgcg gcgctgccct
     5881 tcctgcccgt catggtgtga ggcatttttc tgttatgtcg tttctgttgt tctgatttcc
     5941 ggaggcgggg
//
LOCUS       DAFNBJ010000235         5798 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000235 DAFNBJ010000000
VERSION     DAFNBJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.235"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            449..2734
                     /gene="nrdA"
                     /locus_tag="L2X49_004602"
     CDS             449..2734
                     /gene="nrdA"
                     /locus_tag="L2X49_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBN4799576.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2968..4098
                     /gene="nrdB"
                     /locus_tag="L2X49_004603"
     CDS             2968..4098
                     /gene="nrdB"
                     /locus_tag="L2X49_004603"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBN4799577.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4098..4352
                     /gene="yfaE"
                     /locus_tag="L2X49_004604"
     CDS             4098..4352
                     /gene="yfaE"
                     /locus_tag="L2X49_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBN4799578.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4406..5056)
                     /gene="inaA"
                     /locus_tag="L2X49_004605"
     CDS             complement(4406..5056)
                     /gene="inaA"
                     /locus_tag="L2X49_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBN4799579.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5262..5477
                     /locus_tag="L2X49_004606"
                     /pseudo
     CDS             5262..5477
                     /locus_tag="L2X49_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1466 a         1507 c         1397 g         1428 t
ORIGIN      
        1 caataaccga atagaaaaca accatttcgc catcaacaat ctcttacatt cgcttatata
       61 ttgaccacaa ctgatacatc agattatgtg atgactcgtg cttagatcaa tttttgcaat
      121 cattagcaaa aagattaata aaccatctat atcaatttat ctaacctatt atgccgttca
      181 agaaatcgcc gaacagttat ttttaacaaa tttttctctt cccattgact ttcccggaca
      241 ccttgtctga cctaaggtgc gcgaaagcca ctttttcctt cctgagttat ccacaaagtt
      301 atgcacttgc aagagggtca ttttcacact atcttgcagt gaatcccaaa cataccccct
      361 atatatagtg ttctaagcag cttcccgtac tacaggtagt ctgcatgaaa ctattgcgga
      421 aagaattcca aaaacaggta cgacatacat gaatcagaat ctgctggtga caaagcgcga
      481 cggtagcaca gagcgcatca atctcgacaa aatccatcgc gttctggatt gggcggcaga
      541 aggactgcat aacgtttcga tttcccaggt cgagctgcgc tcccacattc agttttatga
      601 cggtatcaag acctctgaca tccacgaaac cattatcaag gctgccgcag acctgatctc
      661 ccgtgatgcg ccggattatc agtatctagc cgcgcgcctg gcgatcttcc acctgcgtaa
      721 aaaagcctac ggccagtttg agccgcctgc gctgtacgac cacgtggtga aaatggtcga
      781 gatgggcaaa tacgataatc atctgctgga agactacacg gaagaagagt tcaagcagat
      841 ggacaccttt atcgatcacg accgtgatat gaccttctct tatgctgccg ttaagcagct
      901 ggaaggcaaa tatctggtac agaatcgcgt gaccggcgaa atctatgaga gcgcccagtt
      961 cctttatatt ctagttgccg cgtgcttgtt ctcgaactac ccgcgtgaaa cgcgcctgca
     1021 atatgtgaag cgtttttacg acgcggtttc cacatttaaa atttcgctgc cgacgccaat
     1081 catgtccggc gtgcgtaccc cgactcgtca gttcagctcc tgcgtactaa tcgagtgcgg
     1141 tgacagcctg gattccatca acgccacctc cagcgcgatt gttaaatacg tttcccagcg
     1201 tgccgggatc ggcatcaacg ccgggcgtat tcgtgcgctg ggtagcccga ttcgcggtgg
     1261 tgaagcgttc cataccggct gcattccgtt ctacaaacat ttccagacag cggtgaaatc
     1321 ctgctctcag ggcggtgtgc gcggcggtgc ggcaacgctg ttctacccga tgtggcatct
     1381 ggaagtggaa agcctgctgg tgttgaaaaa caaccgtggt gtggaaggca accgcgtgcg
     1441 tcatatggac tacggggtac aaatcaacaa actgatgtat acccgtctgc tgaaaggtga
     1501 agatatcacc ctgttcagcc cgtccgacgt accggggctg tacgacgcgt tcttcgccga
     1561 tcaggaagag tttgaacgtc tgtataccaa atatgagaaa gacgacagca tccgcaagca
     1621 gcgtgtgaaa gccgttgagc tgttctcgct gatgatgcag gaacgtgcgt ctaccggtcg
     1681 tatctatatt cagaacgttg accactgcaa tacccatagc ccgtttgatc cggccatcgc
     1741 gccagtgcgt cagtctaacc tgtgcctgga gatagccctg ccgaccaaac cgctgaacga
     1801 cgtcaacgac gagaacggtg aaatcgcgct gtgtacgctg tctgctttca acctgggcgc
     1861 aattaataac ctggatgaac tggaagagct ggcaattctg gcggttcgtg cacttgacgc
     1921 gctgctggat tatcaggatt acccgatccc ggccgccaaa cgtggagcga tgggtcgtcg
     1981 tacgctgggt attggtgtga tcaacttcgc ttactacctg gcgaagcacg gtaaacgcta
     2041 ctccgacggc agcgccaaca acctgacgca taaaaccttc gaagccattc agtattacct
     2101 gctgaaagcc tctaatgagc tggcgaaaga gcaaggcgcg tgcccgtggt ttaacgaaac
     2161 cacttacgcg aaagggatcc tgccgatcga tacctataag aaagatctgg ataccatcgc
     2221 taatgagccg ctgcattacg actgggaagc tctgcgtgag tcaatcaaaa cgcacggtct
     2281 gcgtaactcc acgctttctg ctctgatgcc gtccgagact tcttcgcaga tctctaacgc
     2341 cactaacggt attgaaccgc cgcgcggtta cgtcagcatc aaagcgtcga aagacggtat
     2401 tttgcgccag gtggtgccgg actacgagca cctgcacgac gcctatgagc tgctgtggga
     2461 aatgccgggt aacgatggtt atctgcaact ggtgggtatc atgcagaaat ttatcgatca
     2521 gtcgatctct gccaacacca actacgatcc gtcacgcttc ccgtcaggaa aagtgccgat
     2581 gcagcagttg ctgaaagacc tgctcaccgc ctacaaattc ggggtcaaaa cactgtatta
     2641 tcagaacacc cgtgacggcg ctgaagacgc acaagacgat ctggtgccgt caatccagga
     2701 cgatggctgc gaaagcggcg catgtaagat ctgatattga gatgccggat gcggcgtaaa
     2761 cgccttatcc ggcctacggc tcggtttgta ggcctgataa gacgcgccag cgtcgcatca
     2821 ggctccgggt gccggatgca gcgtgaacgc cttatccggc ctacggctcg gatttgtagg
     2881 cctgataaga cgcgccagcg tcgcatcagg cacaggatgc ggcgtaaaat gccttatccg
     2941 gcattaaact cccaacagga cacactcatg gcatatacca ccttttcaca gacgaaaaat
     3001 gatcagctca aagaaccgat gttctttggt cagccggtca acgtggctcg ctacgatcag
     3061 caaaaatatg acatcttcga aaagctgatc gaaaagcagc tctctttctt ctggcgtccg
     3121 gaagaagttg acgtctcccg cgaccgtata gattaccagg cgctgccgga gcacgaaaaa
     3181 cacatcttta tcagcaacct gaaatatcag acgctgctgg attccattca gggtcgtagc
     3241 ccgaacgtgg cgctattgcc gcttatttct attccggaac tggaaacctg ggtcgaaacc
     3301 tgggcgttct cagaaacgat tcattcccgt tcctatactc atatcattcg taatatcgtt
     3361 aacgatccgt ctgttgtgtt tgacgatatc gtcaccaacg agcagatcca gaaacgtgcg
     3421 gaagggatct ccagctatta cgatgagctg atcgaaatga ccagctactg gcatctgctg
     3481 ggcgaaggta cccacaccgt taacggtaaa actgtgaccg ttagcctgcg cgagctgaag
     3541 aaaaaactgt atctctgcct gatgagcgtt aacgcgctgg aagcgattcg tttctacgtc
     3601 agctttgctt gttccttcgc atttgcagaa cgcgaattga tggaaggcaa cgccaaaatt
     3661 attcgcctga ttgcccgcga cgaagccctg cacctgaccg gcacccagca tatgctgaat
     3721 ctgctgcgca gcggcgcgga cgatcctgag atggcggaaa ttgccgaaga gtgtaagcag
     3781 gagtgctatg acctgtttgt tcaggcagct caacaggaga aagactgggc ggattatctg
     3841 ttccgcgacg gttcgatgat tggtctgaat aaagacattc tctgccagta cgttgaatac
     3901 atcaccaata tccgtatgca ggcagtcggt ttggatctgc cgttccagac gcgctccaac
     3961 ccgatcccgt ggatcaacac ttggctggtg tctgataacg tgcaggttgc tccgcaggaa
     4021 gtggaagtca gttcttatct ggtcgggcag attgactcgg aagtggacac cgacgatttg
     4081 agtaacttcc agctctgatg gcccgcgtta ccctgcgcat cactggcaca caactgctgt
     4141 gccaggatga acacccttcc cttctggcgg cgctggaatc ccacaatgtg gcggttgagt
     4201 accagtgtcg cgaaggttac tgcggctcct gtcgcacacg cctggttgca ggtcaagttg
     4261 actggattgc cgaaccgtta gcctttattc agccggggga aattttgccc tgttgttgcc
     4321 gggcaaaagg cgatattgaa atcgagatgt gaattggttg tagtgccaga tacaacgctt
     4381 atgcgtctta tctggcctac aacgattaca tggcgtagta atacgctttc acctgctccc
     4441 agtccgcttt ggggattggc tccagatatt tttccagctg gcggaagtca tgattaatcg
     4501 ctttatcgcg acgcaagcgg cgacgacttt tctccaggtc aagaaaacca gcttctgcct
     4561 taccttctgt tttcacatag atatggcgaa cataacaaca gccatgctga cgattaatgc
     4621 tatgcatttt cttaaacgcc agcgccactg ctttcaacat ggcttgccgt acttcgtcag
     4681 aataaggcga tactgcatgc tgggcatacc agtcagcaat gctgatgaac cccgccatat
     4741 cttcagtcac cagcaacgct cgccattcac cctcaatttt caccgcttca ccaaaaacga
     4801 tcttcggcac gatgacaccg gcccgttcca gttctttaat taccgcaacc tcacggacaa
     4861 tcgttggtcg gccgaacgga taacgtacag aatgaaacag atgatgcgtc atgcgcttta
     4921 catacagctt tttgccgttg cgctcgacgc attgcacccc gctcatacca ttacggcgat
     4981 agttaggctc ttcaacccag tcgccctctg ttgcccacca gtgattaaat tcgtcgtact
     5041 ttgctgaaac tgccataccc tatcgcctgt cgtttttatt aacgacaatg actataggtg
     5101 gttacctgag gaaaatctta atgaaacgtg tcgtattaat gaatttcttt tgccgcgact
     5161 agtgtgacac cgcttttgcc cttcatttgc agcgcataga ttccaccagc cacgaccact
     5221 aatagtgcgc ccagcagata aaatgttaat ttaatcagtc ggatacagcg atgaatttta
     5281 ttcgacaggg attaggcatc gccttacaac cagagttaac gctgaaaagc attgcaggtg
     5341 aattgtgttc cgttcctctc gaaccaactt tctatcgaca gatttcgttg ctggctaaag
     5401 aaaagccggt agaaggcagt ccactgtttt tactacaaac gtgtacggaa caactcgtgg
     5461 tgagtggaaa aatttgatgt gagacatgag attgatgaca aatgtaagat tgcctgattc
     5521 gccacactta gttggcctgt agtctttact aggcgtaata attcgttttc acctgatttt
     5581 ggtccacttt gtgaattagc gatatatatt tatccagttt agggaagtga caatgactcc
     5641 aggtagaaac cggtatgaag cgcccgactc actgtcaaaa ccgattgttg agagtaatga
     5701 cactccggcg aacagtacac agtttactct ggagccattc tcaatcttca agtgggatac
     5761 ctaataactt gtcagggaag ttatcaatat ctgtttgt
//
LOCUS       DAFNBJ010000236         5738 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000236 DAFNBJ010000000
VERSION     DAFNBJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.236"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(358..1317)
                     /gene="nrdF"
                     /locus_tag="L2X49_004607"
     CDS             complement(358..1317)
                     /gene="nrdF"
                     /locus_tag="L2X49_004607"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBN4799580.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQLEEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1327..3471)
                     /gene="nrdE"
                     /locus_tag="L2X49_004608"
     CDS             complement(1327..3471)
                     /gene="nrdE"
                     /locus_tag="L2X49_004608"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBN4799581.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVVAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYKIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3444..3854)
                     /gene="nrdI"
                     /locus_tag="L2X49_004609"
     CDS             complement(3444..3854)
                     /gene="nrdI"
                     /locus_tag="L2X49_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBN4799582.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3851..4096)
                     /gene="nrdH"
                     /locus_tag="L2X49_004610"
     CDS             complement(3851..4096)
                     /gene="nrdH"
                     /locus_tag="L2X49_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBN4799583.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4344..4673)
                     /locus_tag="L2X49_004611"
     CDS             complement(4344..4673)
                     /locus_tag="L2X49_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBN4799584.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4825..5169
                     /locus_tag="L2X49_004612"
     CDS             4825..5169
                     /locus_tag="L2X49_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBN4799585.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5206..5655)
                     /gene="alaE"
                     /locus_tag="L2X49_004613"
     CDS             complement(5206..5655)
                     /gene="alaE"
                     /locus_tag="L2X49_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBN4799586.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1467 a         1414 c         1428 g         1429 t
ORIGIN      
        1 catgcaatag aaagattcct ttatttgttt atgtcgatat attacccaat ggaaatagtc
       61 acttttttct accctaacat actgagaatc tgaggcaacc cctgagggca aatgtggcat
      121 gaatattgat gcgagtaaat cgccggaatt tcccgtgata taagggctga gagcaaatcg
      181 agaaaagttg atattttttg tcaaagatag cgaaaaaacg cctgaatatt gtggtttcag
      241 gcgaataatg caacagagta tcagtgtaga tcaccacaaa atatttgcgt gataaaaagc
      301 tatttggcgg gaattatttc cctgtcgcgg gtagtgatat ttttgaaaat aacaccctca
      361 gaaattccag tcttcatctt ctgtttcaac cgctttcccc atcacataag aggagcctga
      421 accggaaaag aaatcgtgat tttcatcggc attcggcgaa agcgcggcga ggattgccgg
      481 attcacttcc gccatttctg cgggaaataa aggttcgtag cccagattca tcaaagcctt
      541 attggcgttg taacagagaa acgctttcac atcgtcagcc cacggggttt cggcgtacag
      601 ctcatcggtg tattgcaact cgttgtcgta gagttccagc aacaaatcga aggcgaaact
      661 cttcaactct tcaagttgtc ccagagatat cttttccatg tttttctgat atttatagcc
      721 tatgtagtaa ccgtgtactg cttcatcgcg gataatcaga cggatcaggt ctgcggtatt
      781 ggtcagcttt ccgcggctgg aaaaatacat cggcagccag aaaccggaat agaacaaaaa
      841 agattcaaga aacacactgg cgattttctt tttcagcgga tcatcaccgc gataatgttg
      901 ctgaataatc tgagcttttc gctgcaacgg tgcgttttct tcactccagg cgtaggcggc
      961 atcgacatct ttggtctggc acagcgtcga gaaaatcgaa ctgtaagagc gggcatgaac
     1021 cgcttccata aagctgatat tcgataatac cgcttcttca tgaggcgtga gtgcatcggg
     1081 catcagcgaa ggcgcgccga taacattttg cagcgtgtcg agcagcgtca ggccagtaaa
     1141 aacgcgcatc gtcagttgtt gttctacgac agttaatgtc tgccaggcag gaatatcgtt
     1201 cgacagcggc accttttctg gtagccagaa attgctggtc aggcgattcc atacctccag
     1261 atctttatcg tcagatatct tgttccagtt gatggcgctg atacgtgaga gtttcataga
     1321 tattccttaa agtgcacagg agacgcagcc ttcaatttca gtgccttcca gcgccatctg
     1381 acgcaggcgg atgtaataga gcgttttgat acctttgcgc caggcgtata tctgcgcttt
     1441 gttgatatcg cgagtggtgg cggtatcggg gaaaaacagc gtcagcgaca gcccctgatc
     1501 gacatggcga gtcgcttccg cgtaggtgtc gatgatcttt tctgcgccaa ttttgtaagc
     1561 gtcctgatac agcgccagat tttcgttagt cataaacggg gcagggtagt aaacgcgtcc
     1621 tgttttgccc tctttgcgta tctctacttt cgccacaatc ggatgaatac tcgacgtagc
     1681 atggttgata taagagatag aaccggttgg cggcaccgcc tggagattct ggttgtatat
     1741 gccgtagcgc atcacgtcgt cgcgcagttg cgcccacatc tcacgggtag gtaacgtaat
     1801 accgctgcgg gcaaacagtt cgccaacttt cgccgttttc ggctgccagt taccttgcag
     1861 atattggctg aaatattcac cactggcata gcgtgactgt ttgaacccgg cgaaggtttc
     1921 accgcgttcg cgcgccagca acatcgaggt acgcagtgcg tgccaggtga tggtatagaa
     1981 atagagattg gtgaaatcca gggcttccgg cgaaccataa gcgatgcctt ctctcgccag
     2041 ataaccatgt aaattcatct gccccagtcc gatggcgtgc gaggcggcat ttccggcttc
     2101 gatggacggc acgctgcgga tatgactcat atctgacacc gctgttaaac cacgtacggc
     2161 agtctctacc gtgcgggcaa agtcggggga atccatggtg tgagcaatat tcaacgaacc
     2221 taaattgcag gagatatcat ggcctgtgcg ggcatagtcg agattctcgt catactctga
     2281 ggcacagttg acctgcaaaa tctctgagca aagattgctc atatttatgc gcccggcgat
     2341 agggttagca cggtttactg tgtcttcata catgatgtag ggatagccgg actcaaactg
     2401 gatttctgcc agtcgctgga agaaatcacg ggcgttgagg tattttttgc ggatgcgttc
     2461 atcggcaacc agttcgtcat agtgttcgct aatggcgata tcggcaaacg gcttgccata
     2521 aactcgctca acgtcatagg gcgaaaacag cgccatctgc gcattctctt ttgccagatg
     2581 gaaagtgata tccgggatca ccacgccaag cgacagtgtt ttaatgcgga ttttttcgtc
     2641 ggcattttcc cgtttcgtgt cgagaaaacg cagaatatcg ggatgatgag catgtaaata
     2701 gactgcacct gccccctgac gcgcgccgag ttggttggca taggaaaatg cgtcttccaa
     2761 cattttcatc accggaatta cgccagaaga ttgattttca atacgtttaa ttggcgcgcc
     2821 cgcttctcgc agattcgaca gcaaaaatgc tacgccgccg ccgcgtttcg acagttgcag
     2881 tgcggaattt accgcccggc caatcgactc catattgtct tcaatacgca gcagaaaaca
     2941 ggaaaccagt tcgccgcgct gctgcttacc gcagttgagg aatgttggcg tggctggctg
     3001 aaagcgtcct gacagcattt catcggtcag ttgcaacgcc agcgtcgcat cgccttgtgc
     3061 cagcgtcagc gccaccatcg tgactcgatc ggcaaaatct tccagataac gtttaccgtc
     3121 gaatgtcttc aacgtatagc tggtgtaaaa cttccatgcc ccgaggaatg tctggaaacg
     3181 aaaaccgctg gtgtgtgcgt gggcaaacag cgtaatgaca aagtcgcgag agtagcgatt
     3241 aagaacgctt tcatcatagt aaccttcgtt gaccagccag ttcaggcgct gctgctggct
     3301 actgaaggtc acactgttcg gacgcacatg cgtcgccata aaggcgacaa cggcctggcg
     3361 gtctttatcg aactgaatgc gacctgcgct atcgtagagg ttaagcatcg cattcagcgc
     3421 gtggtaatcc atcgtttcct gcgtcaggca ttctgcggtt gtcgttgcca aaattcggtt
     3481 actcctttac gaacgttttc gatatcgctt tgcgtaccca tgagttcaaa acggtacagc
     3541 cacggcacgc cgcatttccg ggcaatcaca tctccggcgc ggccatacgc ctcaccaaag
     3601 ttgcgattac cagaagcaat aacgccgcga agcaacgccc ggttgtgctc gtcgtttaaa
     3661 aagcgaatta cctgtcgtgg caccgcgcca gccgtaccgc cgccgccgta agagggcacg
     3721 atcaggatgt aaggctcgtc tacctgaatc cgttcccgct cattgagcgg gatgcgcacc
     3781 gcgggcagac ctaaacgttc gataaaacgc tgcgtgtttt cggagctgct ggagaagtag
     3841 acgagctggc tcatgcactg gccgcgtgtg gcgctggatg cagacggtta atcatgtccg
     3901 gacggaaacc agaccagcta agatcgccag caatcactac cggcaactga cgaaagccct
     3961 gagcacgcaa cgcttccgct gcttcaggaa cgcgatcgac attaatcatt tcaaaatcaa
     4021 agccccggtt ttccatcgcc cgtttggtgg cgtggcactg aacgcaatcg ttacgagtgt
     4081 aaatagtaat gcgcatgatt cgtatttccg tttaaaatga agatacggcg cgatgatacg
     4141 cgtcgggttg tctctctgtt gatacagaga tactagatgt agttaaaaaa agattcaacc
     4201 acacaatata tagcaaatag tggtcgaaat taccctggat atgagcgtga cggggtaggg
     4261 ggatttttgt gattcaccag gcaaaaagaa accccgaaga caggcttcgg ggtcaaagac
     4321 gcgtatttat tatcattttt gcactacgat ttgcgcatgc ttaacagtgc gccgataaaa
     4381 atacctaccg cagctgctgt acccacgcta caccagggtc tttcacgaac aaaagaatcc
     4441 gcgcagccaa cggcatcgcg cgcggcttgc tggacgcgag tacgaccatg cattcgcgcc
     4501 ctggtttctt tcagcaacgc ctgagcttta ctgcgtgcgg cttcagcttc ccctttggcg
     4561 tcgctgcccc aggacttcaa tacagattcg aggctgtcag ctaattgatt gacatcattc
     4621 tggatatcct gcacgccatc atcaacgtcg ttgcggttcg gtcggttaaa catatgctcc
     4681 cccattagaa ttcgatgtaa ttttcagtgt agaccataat tttcttaact gaggttcatc
     4741 gcggctttat ggacttttct gaaagcgttg tgaatgcgca atcaggtaag gtaggcccgc
     4801 ggttgataat aacgaggatc aaatatgtat ttacgaccag acgaggtggc gcgcgtactt
     4861 gaaaaagtcg gttttactgt cgatgtggta acccagaaag cgtatggtta ccgccgtggg
     4921 gaaaattatg tctatgttaa tcgcgaagcg cgaatgggac gtaccgctct ggtaattcat
     4981 ccaacattaa aagaacgcag ttcaacactg gctgaacccg cttccgatat taaaacttgc
     5041 gatcattacc agcaatttcc gctctattta gcaggcgagc gacacgagca ttacggtatc
     5101 ccgcatggct tcagttcgcg tgttgcgctt gaacgttatt tgaatggttt atttggcgaa
     5161 gccagttaaa gacgcgactg gcgatgccag tcgcgaaaag aagagtcagg cttttacctg
     5221 ctggtaacgg ctgactttaa acagtcggcg gcaataatcg aggaagtagc cataaaccgc
     5281 ccccatcaac atcgaaacaa cgatgtttga actgaccgcc gccataatct gatgccagtc
     5341 tgcgcccacc actaacaaga tcgccacata caccggtgac tggaacgtca cataagccag
     5401 aatatccgcc agatttttta tccagcccga cgggctaact ttgcgtgccg cgcgcataaa
     5461 cagatcacgg tacataccgt atggccatgc aattaagatg ttcaccggaa tcgctaccaa
     5521 tctggaataa aaagactgtt cgaagctcat tccggagagg aaaacttcaa tacacatgtt
     5581 cacgacagaa cagtaaacaa ccatcgcgaa cgtatctgca actgcatgac gcaagcgtga
     5641 ctgcggtgag aacatgctaa tgctccttaa taaaagcgta atgggatgtt aatggagatg
     5701 aaagcagtgg atctcattgc catctgcatt ttcgcata
//
LOCUS       DAFNBJ010000237         5656 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000237 DAFNBJ010000000
VERSION     DAFNBJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.237"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(61..372)
                     /locus_tag="L2X49_004614"
     CDS             complement(61..372)
                     /locus_tag="L2X49_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN4799587.1"
                     /translation="MKKIMLARAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            571..1269
                     /locus_tag="L2X49_004615"
     CDS             571..1269
                     /locus_tag="L2X49_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN4799588.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1314..2213)
                     /gene="eamA"
                     /locus_tag="L2X49_004616"
     CDS             complement(1314..2213)
                     /gene="eamA"
                     /locus_tag="L2X49_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBN4799589.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLMLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2408..3595
                     /gene="ydeE"
                     /locus_tag="L2X49_004617"
     CDS             2408..3595
                     /gene="ydeE"
                     /locus_tag="L2X49_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBN4799590.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3722..3817
                     /gene="mgtS"
                     /locus_tag="L2X49_004618"
     CDS             3722..3817
                     /gene="mgtS"
                     /locus_tag="L2X49_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBN4799591.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(4036..4926)
                     /gene="dgcZ"
                     /locus_tag="L2X49_004619"
     CDS             complement(4036..4926)
                     /gene="dgcZ"
                     /locus_tag="L2X49_004619"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HBN4799592.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(5181..5573)
                     /gene="ydeI"
                     /locus_tag="L2X49_004620"
     CDS             complement(5181..5573)
                     /gene="ydeI"
                     /locus_tag="L2X49_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBN4799593.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1552 a         1342 c         1180 g         1582 t
ORIGIN      
        1 atatccattt tatgatgtgt gaaaagttag ttcaggtaat agtttaataa tattagattc
       61 ttactcttgc agtagtttca tcgcaaattc aagaactgcc tgtccgttca tggtgccgta
      121 gtctttcatc tcgataacgg ctacaggcat tttcccctgt gcaacggctt caatctctgg
      181 tttctgaaag cgaacttgcg ggccaagtaa aacaacgtca acttcgttat ttttaatttt
      241 ccctttagct tctgcaatcg ccaatgcgga aatattaact tcaagtgaaa tagcgttagc
      301 atgatcaatc atacgtttaa ccagcatact ggttgacatg cccgcagcgc gggctaacat
      361 tatttttttc atattttccc ttactggtat ataacagatt acattgttta aatatcatat
      421 cccgcagtag ctatacgtcc tggtacattg atcacagtaa tacagtgata aagttgttca
      481 acatcagggt gattattaaa cgactcggcc atacgataaa ccaatcagga ctcaactcct
      541 tgttttcaat ggctcttaaa aggtgcaaaa gtgatttttc aaaagattgc ccgcttactt
      601 aaatccgaaa tcaatggcaa ttcatggcat gttggtgatt tgttgccgtc agaagcggaa
      661 ctcgctgttc gttataatgt ttcgcgtaat accctgcgta aggcattatc cttgctggaa
      721 ggcgaaggaa ttattcacag aaagcatggt tcaggaacat acattcaaaa aaagaatttt
      781 gttgcacaca ttgatcacat gaacagtttc agtgaaattg cgcataaaag cggcaaagag
      841 gcagggagtc agattatgaa atttgaagtg caggatgctt cacccactat tgcgactgag
      901 ctgaatttag tgactggtga gcaggtttat tacataaaga gactgcgatt cattgaggat
      961 aatgcagcac aattagaaga aacgtggatg tcagtagcac gttttcctga tttaaccgtg
     1021 tcacatatgc aaaaatccaa attttcatat attgagaatg aatgcgggat caaaatcatt
     1081 ggcacctttg aaactttctc ccctactttt cctaccccag aaatcgccag tattttacgg
     1141 atcagcccac gggatcccat acttaaaatt cagacccagg ctgtggacag taactctatt
     1201 ccgctggatt attcgttact ttacagcaat attttcgagt tccaggtaaa gtactttttc
     1261 ccgcgataaa agcagacgca cagagtgctg acatcacatt ctgtgcgtat ggtttagcct
     1321 ctaaccttta ccgctttacg ccaccgcaag ccaaatacat tgatatacag cccggtcata
     1381 atgagcaccg cacctaaaaa ttgcagaccc gttaagcgtt catccaacaa tagtgccgca
     1441 cttgccagtc cgactacggg caccagtaac gataacggtg caacccgcca ggtttcatag
     1501 cgtcccagta acgtccccca gatcccataa ccaacaattg tcgccacaaa cgccagatac
     1561 atcagcgaca aaatggtggt catatcaata gtaaccagac tgtgaatcat ggatgcggaa
     1621 ccatcgagaa tcagcgaggc aacaaagaag ggaatgattg ggattaaagc gctccagatt
     1681 accagcgaca tcaccgccgg acgcgttgag tgcgacatga tctttttatt gaagatgttg
     1741 ccacacgccc aactaaatgc tgcagccagg gtcaacataa agccgagcat cgccacatgc
     1801 tgaccgttca gactatcttc gattaacatc agtacgccaa aaatcgctaa ggcgatcccc
     1861 gccaattgtt tgccatgcag tcgctccccg aaagtaaacg cgccaagcac gatagtaaaa
     1921 aacgcctgtg cctgtaacac cagcgaagcc agtccagcag gcataccgaa gttaatggca
     1981 caaaaaagaa aagcaaactg cgcaaaacta atggttaatc cataccccag cagcaaattc
     2041 agtggtactt tcggtcgtgc gacaaaaaag atagccggaa aagcgaccag cataaagcgc
     2101 aaaccggcca gcatcagcgg tggcatgtta tgaagcccca ctttgatgac cacaaaattt
     2161 agcccccata cgaccactac cagtagcgcc aacaccccat cttttcgcga cattctaccg
     2221 cctctgaatt tcatcttttg taagcaatca acttagctga atttactttt ctttaacagt
     2281 tgattcgtta gtcgccggtt acgacggcat taatgcgcaa ataagtcgct atacttcgga
     2341 tttttgccat gctatttctt tacatctcta aaacaaaaca taacgaaacg cactgccgga
     2401 cagacaaatg aacttatccc tacgacgctc taccagcgcc cttcttgcct cgtcgttgtt
     2461 attaaccatc ggacgcggcg ctacgctgcc atttatgacc atttacttga gtcgccagta
     2521 cagcctgagt gtcgatctaa tcggttatgc gatgacaatt gcgctcacta ttggcgtcgt
     2581 ttttagcctc ggttttggta tcctggcgga taagttcgac aagaaacgct atatgttact
     2641 ggcaattacc gccttcgcca gcggttttat tgccattcct ttagtgaata acgtgacgct
     2701 ggtggtgctc ttttttgccc tcattaactg cgcctattct gtttttgcta ccgtgctgaa
     2761 agcctggttt gccgacaatc tttcgtccac cagcaaaacg aaaatcttct caatcaacta
     2821 caccatgctc aacattggct ggaccatcgg tccgccgctc ggcacgctgt tggtgatgca
     2881 gagcatcaat ctgccctttt ggctggcagc tatctgttcc gcgtttccca tgcttttcat
     2941 tcaaatttgg gtaaagcgca gcgagaaaat catcgccacg gaaacaggca gtgtctggtc
     3001 gccgaaagtt ttattacaag ataaagcact gttgtggttt acctgctctg gttttctggc
     3061 ttcttttgta agcggcgcat ttgcttcatg catttcacaa tatgtgatgg tgattgctga
     3121 tggggatttt gccgaaaagg tggtcgcggt tgttcttccg gtgaatgctg ccatggtggt
     3181 tacgttgcaa tattccgtgg gccgccgact taacccggct aacatccgcg cgctgatgac
     3241 agcaggcacc ctatgtttcg tcatcggtct ggtcggtttt attttttccg gcaacagcct
     3301 gctattgtgg ggtatgtcag ctgcggtatt tactgtcggt gaaatcattt atgcgccggg
     3361 cgagtatatg ttgattgacc atattgcgcc gccaggaatg aaagccagct atttttccgc
     3421 ccagtcttta ggctggcttg gtgccgcgat taacccatta gtgagtggcg tagtgctaac
     3481 cagcctgccg ccttcctcgc tgtttgtcat cttagcgttg gtgatcattg ctgcgtgggt
     3541 gctgatgtta aaagggattc gtgcaagacc gtgggggcag cccgcgcttt gttgatttaa
     3601 gtcgaacaca ataaaaattt aattcagcct tcgtttaggt tacctctgct aatatctttc
     3661 tcattgagat gaaaattaag gtaagcgagg aaacacacca caccataaac ggaggcaaat
     3721 aatgctgggt aatatgaatg tttttatggc cgtactggga ataattttat tttctggttt
     3781 tctggccgcg tatttcagcc acaaatggga tgactaatga acggagataa tccctcacct
     3841 aaccggcccc ttgttacagt tgtgtacaag gggcctgatt tttatgacgg cgaaaaaaaa
     3901 ccgccagtaa accggcggtg aatgcttgca tggatagatt tgtgttttgc ttttacgcta
     3961 acaggcattt tcctgcactg ataacggatc gttgacacag tagcatcagt tttctcaatg
     4021 aatgttaaac ggagcttaaa ctcggttaat cacattttgt tcgtcaataa acatgcagcg
     4081 atttcttccg gtttgcttac cctcatacat tgcccggtct gctcttccaa tgaccacatc
     4141 cagaggctct tcaggaaatg cgcgactcac acctgctgtc acggtaatgt tgatatgccc
     4201 ttcagaatgt gtgatggcat ggttatcgac taactggcaa attctgacac ctgcacgaca
     4261 tgcttcttca tcattagccg ctttgacaat aatgataaat tcttcgcccc cgtagcgata
     4321 aaccgtttcg taatcacgcg tccaactggc taagtaagtt gccagggtgc gtaatactac
     4381 atcgccgatt aaatgcccgt aggtatcatt aaccaattta aatcggtcaa tatccaacaa
     4441 cattaaataa agattcagag gctcagtgtt gcgtaactga tgatcaaagg attcatcaag
     4501 aacccgacgc cccggcaatc ccgtcaaaac atccatattg ctacggatcg tcagcaaata
     4561 aattttgtaa tcggttaatg ccgcagtaaa agaaagcaac ccctcctgaa aggcgtcgaa
     4621 atgcgcgtcc tgccagtgat tttcaacaat agccagcatt aattcccgac cacagttatg
     4681 catatgttga tgggcagaat ccattagccg aacgtaaggt aattcatcgt tatcgagtgg
     4741 ccccagatga tcaatccacc gaccaaactg gcacagtcca taagaatggt tatccgttat
     4801 ttctggctta ctggcatctc tcgcgaccac gctgtgaaac atactcacca gccactggta
     4861 gtgggcatcg atagccttat tgagatttaa caagatggca tcaatttccg ttgtcttctt
     4921 gatcattgcc actccttttt cacagttcct tgtgcgcgct attctaacga gagaaaagca
     4981 aaattacgtc aatattttca tagaaatccg aagttatgag tcatctctga gataacattg
     5041 tgatttaaaa caaaatcagc ggataaaaaa gtgtttaatt ctgtaaatca cctctgcatt
     5101 atcgtaaata aaaggatgac aaatagcata acccaatacc ctaatggccc agtagttcag
     5161 gccatcaggc taatttattt ttatttctgc aaatgagtga cccgaacgac ggccggcgca
     5221 cttttcttat ccagactgcc gctaatgttg atcatctggt ccggctgaac ttctcgtcca
     5281 tcaaagacgg ccgcaggaat aacgacatta atttcaccgc tcttatcgcg aaaaacgtaa
     5341 cggtcctctc ctttgtgaga aatcaaatta ccgcgtagtg aaaccgaagc gccatcgtgc
     5401 atgttttttg cgaaatcaac ggtcattttt tttgcatcat cggttccgcg atagccatct
     5461 tctattgcat gaggcggcgg tggcgctgca tcctgtttta aaccgccctg gtcatctgcc
     5521 aacgcataag gcatgacaag aaaacttgct aatacaatgg cctgaaattt catactaact
     5581 ccttaattgc gtttggtttg acttattaag tctggttgct atttttataa ttgccaaata
     5641 agaatactgc caattg
//
LOCUS       DAFNBJ010000238         5569 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000238 DAFNBJ010000000
VERSION     DAFNBJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.238"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..1418
                     /gene="norV"
                     /locus_tag="L2X49_004621"
     CDS             <1..1418
                     /gene="norV"
                     /locus_tag="L2X49_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBN4799594.1"
                     /translation="IHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEKNVLIDTVD
                     HKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYCTANAIDSI
                     NGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDAVLFSNDAF
                     GQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNLPVDMIATS
                     HGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGIAETDPRVA
                     VKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLRFRNKRASA
                     FGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALKLCREHGREIARQWALAP
                     LPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGTPWSEVPDN
                     FLCPECSLGKDVFEELASEAK"
     gene            1415..2548
                     /gene="norW"
                     /locus_tag="L2X49_004622"
     CDS             1415..2548
                     /gene="norW"
                     /locus_tag="L2X49_004622"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBN4799595.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGIQA
                     TLDRQRNIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(2676..4928)
                     /gene="hypF"
                     /locus_tag="L2X49_004623"
     CDS             complement(2676..4928)
                     /gene="hypF"
                     /locus_tag="L2X49_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBN4799596.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSFEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAHEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRFFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(5081..>5569)
                     /gene="hydN"
                     /locus_tag="L2X49_004624"
     CDS             complement(5081..>5569)
                     /gene="hydN"
                     /locus_tag="L2X49_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBN4799597.1"
                     /translation="GCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATV
                     CRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNS
                     GAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKLEQLSAEKRRRTAL
                     MF"
BASE COUNT         1325 a         1589 c         1500 g         1155 t
ORIGIN      
        1 acattcattg ggttggtcaa cgtgactggg aagtgcgtga ttttcacggt acggaatata
       61 aaacgctgcg cggcagcagt tacaacagct acctcatccg cgaagaaaaa aacgtgctga
      121 tcgacaccgt cgaccataaa ttcagccgcg aatttgtgca gaacctgcgt aatgaaatcg
      181 atctggcgga tatcgattac atcgtgatta accatgcaga agaggaccac gccggggcgc
      241 tgaccgaact gatggcacaa attcccgata cgccgatcta ctgtacagcc aacgctatcg
      301 actcgataaa tggtcatcac catcatccgg agtggaattt taatgtggtg aaaactggcg
      361 acacgctgga tatcggcaac ggcaaacagc tcatttttgt cgaaacacca atgctgcact
      421 ggccggacag catgatgact tacctgacag gcgacgcggt gctgttcagt aacgatgctt
      481 tcggtcagca ctactgcgac gagcatctgt tcaacgatga agtggatcag acggagcttt
      541 tcgagcagtg ccagcgttac tacgccaata tcctgacgcc gttcagccgc ctggtaacac
      601 cgaaaattac cgagatcctg ggctttaact taccagtcga tatgatagcc acttcccacg
      661 gcgtggtatg gcgcgataac ccgacgcaaa ttgtcgagct gtacctgaaa tgggcggctg
      721 attatcagga agacagaatc accattttct acgacaccat gtcgaataac acccgcatga
      781 tggctgacgc tatcgcccag gggattgcgg aaaccgaccc acgcgtggcg gtgaaaattt
      841 tcaacgtcgc ccgaagcgat aaaaacgaaa tcctgactaa tgtcttccgc tcaaaaggcg
      901 tgctggtcgg cacttcgacg atgaataacg tgatgatgcc gaaaatcgcc gggctggtgg
      961 aggagatgac tggtttacgc ttccgtaaca aacgcgccag tgctttcggc tctcacggct
     1021 ggagcggcgg tgcggtggat cgtctttcca cgcgcctgca ggatgcgggt ttcgaaatgt
     1081 cgcttagcct gaaagcgaaa tggcgaccag accaggacgc tctgaagtta tgccgtgaac
     1141 acggtcgcga aatcgcccgt cagtgggcgc tcgcgccgct gccgcagagc acggtgaata
     1201 cggtagttaa agaagaaacc tctgccacca cgacggctga cctcggccca cggatgcagt
     1261 gcagcgtctg ccagtggatt tacgatccgg caaaaggcga gccaatgcag gacgttgcgc
     1321 caggaacgcc gtggagtgaa gtcccggata acttcctctg cccggaatgc tccctcggca
     1381 aagacgtctt tgaagaactg gcatcggagg caaaatgagt aacggcattg tgatcatcgg
     1441 ttcgggcttc gccgcccgcc aactggtgaa aaatattcgc aaacaggacg ccactattcc
     1501 attaaccctg attgccgccg acagcatgga tgagtacaac aaacctgacc tcagccatgt
     1561 tatcagtcag gggcaacgtg ccgatgacct tacccgccag acggcgggtg aatttgccga
     1621 gcagtttaat ctgcacctgt ttccacaaac ctgggtgacg gatatcgatg ccgaagcccg
     1681 tgtggtgaaa agccagaata atcagtggca atacgacaag ctagtactgg caaccggtgc
     1741 cagtgccttt gtcccgcctg tgcctgggcg tgagttaatg ctgacgttaa atagtcagca
     1801 agagtatcgc gcctgtgaaa cgcaactgcg ggatgcccga cgcgtgttga ttgttggcgg
     1861 tggtttgatt ggtagcgaac tggcgatgga tttttgtcgt gcaggcaaag cggtcacgct
     1921 aatcgacaac gctgccagta ttctggcgtc gttaatgcca ccggaagtaa gcagccgctt
     1981 gcagcatcgg ttgacggaga tgggcgttca tctgctgttg aaatctcagt tacaggggct
     2041 ggaaaaaacg gattctggca ttcaggcaac gctggaccgc cagcgcaata tcgaagtgga
     2101 tgcggtaatt gccgccaccg gactgcgccc ggaaaccgcc ctggcacgac gcgccgggct
     2161 gacgattaat cgcggcgttt gcgtcgatag ttatctgcaa accagtaata ccgatattta
     2221 cgcgctgggc gattgcgcgg aaattaacgg tcaggtattg ccgttcctcc agccgattca
     2281 acttagcgcg atggtgctgg caaaaaatct tctcggcaat aacacgccgc tgaaactccc
     2341 ggcgatgctg gtgaaaatca aaacgccgga attaccgctg catctggcag gcgaaaccca
     2401 gcgtcaggat ttacgctggc aaattaatac cgaacgccag ggaatggtgg cgcgcggcgt
     2461 tgacgatgca gaccagcttc gcgcctttgt ggtcagtgag gatcggatga aagaggcatt
     2521 tggattgttg aaaacattgc cgatgtaggt gggctactgt gcctaaaatg tcggatgcga
     2581 cgctggcgcg tcttatccga cctacgggga cgcatgtgta ggccggataa ggcgtttacg
     2641 ccgcatccgg caatggtgtc caaatgcaac acgttttatc cgttctggac ttcacccgct
     2701 aaccaacgcg ccgcagcaat aaccccctgc cccagagaca aaccgccatc acccgccggt
     2761 aaactctgtg gaaagagcaa tgtgaaatca gcgagataat gcgccagacg tgcacgcagc
     2821 aaacggttat gaataacccc gccgctaaat accagcgtag tgataccacg catcgtggcc
     2881 tgctcacgca tcaacgcggc aaaaccctgc gccagcgcat catgaaacgc ccacgcgcgt
     2941 tgattaaccg gtgcctgcca gttcagccac tgctgccaga aagtggcgag atccagttga
     3001 ttgtccaccc gcggcattgt caccggatgc gtcactccgt ggcatgaggc tgcgagcgcc
     3061 tccagagcac aagccgcttc accttcataa cttaacgtgg ctggcgcaca gcccagtgcc
     3121 gccgccactg catcgaaaaa acgcccacac gatgacgcca gcggcgcgtt aattccacgc
     3181 tcaatggccc gcgccagcac gctccagttt tgctgttgca cactcgctgt ttcgggataa
     3241 ttctgccact ccggcacaaa gcgcaggcac tgcgccagca ggtttcgcca cggctgcttc
     3301 gccgccaaat cgccaccagc aagcgccact gcaggcaagc cgcccaggtg ctcacattcg
     3361 cgatagttca cccgcaggca ctcgccgccc cacaaagcgc cattctcccc cataccgata
     3421 ccgtcgagcg tcaaagcaat gacatcaccg ccatccagcg gccactgatg ctctgccaga
     3481 cacgccgctg cgtgggcatg atgatgcagc accgtttgcg tcggcagatt catttcgtgc
     3541 gcccactggc tggagacata gcccggatgt gcgtcatgca caacgtattg cggggtgaaa
     3601 tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact gcatctggat gccatcgtca
     3661 cttaaatcgc ccagatgctg actcaacacc gcttgttcac cgcgcaccag gcagaaggta
     3721 tttttcagat ccgcgccgag acacagcaca ggcggaacat ttttaaagcc cggaggcaaa
     3781 gccagcgcat ccggcacata cccgcgtgaa cggcgcagca tttcgccgct ttcgcgcacc
     3841 actgaatcat ccatccgctg cacgatgtcg cggttatgta ttaagaatcc gtcggcaatg
     3901 ccctgcaaat ccgccagcgc ctgttcgttg ctgatagccg gtggtttacc gctcaggttg
     3961 ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca gcaaatgctg gagcgggttc
     4021 gcaggcaaca tcaccccgac ttcgttaagg ccaggggcga tatcatcaca aagctcagga
     4081 acgtattttt tatccaccag cacaatcggc gcggcgggcg tggtaagcaa ctggcgcgca
     4141 gcgtctggta aaccgtcagc cactggcaac atgaccgcca gcggtttcgc cggacgatgt
     4201 ttgcgcgccc gaagtgtcgc caccgcgtta ctgttacgtg catcgcaggc aagatgaaat
     4261 ccgccaatcc ctttgatggc gacaatgttg cccattttta actgtgcgat agccgcctgt
     4321 aatgccgcct cttgttctgc atgttcacca tgacttaccc attcaagatg tgggccacac
     4381 tccgggcagg ccaccggctg ggcgtggaag cgacgatcga gcgggtcgcg gtactcttta
     4441 tcacaggctg gacacagcgg aaactccgcc attacggtaa acgggcggtc gtaaggcatg
     4501 gcgcgaataa tggtgaaacg cgggccgcag tgggtacagt tgataaacgg ataacgataa
     4561 cgccgttcgc ctggggtatt catttcggca aggcaagcag ggcaagtagc ggcatccggg
     4621 acaatttgcg tattcatggc acctcccgcg ctctggcgga tagtgaactc ggtgggcagt
     4681 tgtgaccaga taaacggctc acgctcgaag ctatcaatac gcgccagtgg cggacagtgc
     4741 tgatgcaatt gaacaagaaa cgtttccggg tcttccagca gccggacttc tacgccatcg
     4801 ccgtcattac agacatcgcc gtgaagattt aattgctgtg ccagctgcca gacaaacgga
     4861 cgaaaaccga cgccctgcac tttgcctcga atacgcagtt ggacaccgca agatgtgttt
     4921 tttgccattg agttattccc gccatcatga attgcgtaac ccgccctgcc ggacacgact
     4981 gcgtcgtatc cggcagtctc aggtcggcga taccgccagc tccgtattct acgaatattt
     5041 ccgggaattc ctttgatgcc agaacagttc tataagattt ttagaacatc agcgccgtac
     5101 ggcgacgttt ttctgcgctc agttgttcaa gtttattacg atcgacacaa atcagcgcat
     5161 gggtcgggca agccgccata cacgccgggc cgtcttcgcg atggttgcac aggtcgcatt
     5221 tattggcttc agctttgtca gcccgtacat tcagccccgc gccgctgttg cggatcaccg
     5281 gacgtaccac cacttccatc gcgccatacg ggcaagccac cacgcaggtt ttgcaaccaa
     5341 tgcaacgttc ctgcatcaca tgaacaaacc ctttatcacg gctgatagca ccattcgggc
     5401 agacgttagc gcacggtgca tcttcacact gacggcaaac tgtcgccgtg gaaatgttca
     5461 cacctttaat gacatggata cgcggtaaaa atgtttccgg ggtcagcgat gcacagtcct
     5521 gattttcctg atgagaaacc acgcacgcca cttcacaggt acggcaacc
//
LOCUS       DAFNBJ010000239         5558 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000239 DAFNBJ010000000
VERSION     DAFNBJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.239"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            61..495
                     /locus_tag="L2X49_004625"
     CDS             61..495
                     /locus_tag="L2X49_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBN4799598.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            850..1230
                     /locus_tag="L2X49_004626"
     CDS             850..1230
                     /locus_tag="L2X49_004626"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBN4799599.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            1227..1574
                     /gene="tnpB"
                     /locus_tag="L2X49_004627"
     CDS             1227..1574
                     /gene="tnpB"
                     /locus_tag="L2X49_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBN4799600.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            1624..3009
                     /locus_tag="L2X49_004628"
     CDS             1624..3009
                     /locus_tag="L2X49_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBN4799601.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3248..4606)
                     /locus_tag="L2X49_004629"
     CDS             complement(3248..4606)
                     /locus_tag="L2X49_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HBN4799602.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            4888..5019
                     /locus_tag="L2X49_004630"
                     /pseudo
     CDS             4888..5019
                     /locus_tag="L2X49_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5339..>5558)
                     /locus_tag="L2X49_004631"
     CDS             complement(5339..>5558)
                     /locus_tag="L2X49_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HBN4799603.1"
                     /translation="LANILYEANSQFFYERNVIEEAVNALFCEREIINNKNIIKKLMF
                     FLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         1312 a         1437 c         1487 g         1322 t
ORIGIN      
        1 gtactgcaca cactgccaga ccggaggatt cagtattccc cggacaactc aggaggaaat
       61 atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa
      121 gaagctgcga agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta
      181 acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc
      241 gctgaaaaaa tcctgctgcg atgtacctgg cttgacccgg cggagaaaga ggcccgtcgt
      301 ttgcatctgc agggcatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt
      361 gtgatgtgtg acgaagatta tgagggggaa aagtgctgcc cacgctgcgg taccggggta
      421 tacagcaggg cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg
      481 ttacagaggc tgtaatggtt gcctccggag cagtttgcgg tgatgccttt cccgggaatg
      541 gattgccagt gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccgttgattt
      601 tatgccaaca gccccctgtg actgacaggc tgcctcgtca ttccattcgt atctccagta
      661 acagggggtt gtattgtatt ttattgtgcc ggttcaggtg tgcgtttccc ggcgcgtctg
      721 caccggctta accaaattca acagggataa aatagtggta agcgtacagc ctgaaccgtc
      781 tggtcagaat ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaaccaaa
      841 gtgtcagata tgcagaaaaa tgtgactccc ggcaggcgaa aaggctgccc taattatcct
      901 cccgaattta aacagctgct cgttgctgcc tcctgtgaac ccgggatatc catctcaaaa
      961 cttgctcttg aaaatggcat taacgccaat ctgttgttca aatggcgaca acaatggcgc
     1021 gagggaaagc tgctattacc ttcttcagag agcccccagc tacttcctgt gactctcgat
     1081 gcagctgccg aacagccaga atcgctcgca gaggacccgg aaaccctcag tatcagctgt
     1141 gaggtaacgt tccggcacgg gacgctccgc ttcaatggca atgtcagcga aaagctcctg
     1201 actctgctga tacaggaact gaagcgatga tcccgttacc ttccgggacc aaaatttggc
     1261 tggttgccgg tatcaccgat atgagaaatg gcttcaacgg cctggctgcg aaagtacaaa
     1321 cggcgctgaa agacgatccc atgtccggcc atgttttcat tttccggggc cgcagcggca
     1381 gtcaggttaa actgctgtgg tccaccggtg acggactgtg cctcctgacc aaacggctgg
     1441 agcgtgggcg cttcgcctgg ccgtcagccc gtgatggcaa agtgttcctt acgcaggcgc
     1501 agctggcgat gctgctggaa ggtatcgact ggcgacagcc caagcggctg ctgacctccc
     1561 tgaccatgct gtaaatctct ttatcctggt tgtcacagaa taagcccggt aaaatacggg
     1621 cttatgaacg acatctcttc tgacgacatc ttcctgctaa aacagcgcct ggccgaacag
     1681 gaagcgctga tccacgccct gcaggaaaag ctgagcaacc gggagcgcga aatagaccat
     1741 ctgcaggcgc agctggataa actccgccgg atgaacttcg gcagtcgttc cgaaaaagtc
     1801 tcccgccgta tcgcacaaat ggaagccgat ctgaaccggc ttcagaaaga gagcgatacg
     1861 ctgactggta gggtgtatga cccggcagta cagcgtccgt tgcgtcagac ccgcacccgt
     1921 aagccgttcc ctgaatcact accccgtgac gaaaagcgac tgttgcctgc ggcgccgtgc
     1981 tgcccgaact gcggcggttc actgagctat ctgggcgagg ataccgccga acagctggag
     2041 ttgatgcgta gcgcattccg ggttatccgg acggtacggg aaaaacatgc ctgtactcag
     2101 tgcgatgcca tcgtgcaggc acctgcacct tcgcggccca tcgagcgggg tatcgccgga
     2161 ccggggctgc tggcccgcgt gctgacctcg aagtatgcag agcacacccc gctgtattgc
     2221 cagtcagaaa tatacggccg gcaaggtgtg gagctgagcc gttcactgct gtcgggctgg
     2281 gtggatgcat gctgccggct gctgtctccg ctggaagagg cgcttcatgg ctatgtcatg
     2341 actgacggca aactccatgc cgatgatacc ccggtccagg tactgctgcc gggtaataag
     2401 aagacgaaga ccgggcggtt gtgggcgtat gttcgtgatg accgcaatgc cgggtcagcg
     2461 ttggcacctg cagtgtggtt cgcttacagc ccggacagaa aaggcatcca tccgcagact
     2521 catcttgctt gcttcagcgg tgtgctgcaa gcggatgcgt acgccgggtt caacgagctg
     2581 tatcgcaatg gtgggataac ggaagctgcc tgctgggctc atgcccgccg aaagatccac
     2641 gatgtgcacg tccgcatccc gtcagcactg acggaagaag ccctggagca gatcggtcag
     2701 ttgtacgcca tagaggcgga tataagggga atgccggcag agcagcggct tgctgaacgt
     2761 cagcgaaaaa cgaaaccgct gttgaaatcc ctggaaagct ggttgcgtga aaagatgaag
     2821 accctgttct tcggctctgg ccatggtggt gagcggggag cgctactgta cagcctgatc
     2881 gggacgtgca aactgaatga cgtggatcca gaaagctacc ttcgccatgt gcctggcgtc
     2941 atagcagact ggccggtcaa ccgggtcagc gaactgcttc cgtggcgcat agcactgcca
     3001 gctgaataac acatccccgt caatacggcc ctcgctgtac gcttactttt gttcggactg
     3061 cttatggcct ttttccactg gtgtagcagg aaagttaacc ggctgccacc atgagatgac
     3121 gtgtaaaccg tgcaggcatg ttttcacgtg gtgacgcttt cattgggggt ctacgccgga
     3181 accccaatat tgttcacata catgaatttg aaaaactaag cgatactgct gtccgtacct
     3241 tataaactta tttcaacgct tccggcagcg tcacaatcca cagttcactg tcagactccg
     3301 gtttctccag tggcttcact ctgagggtcg tttcaacctg cttgtcgtca agcgtcagct
     3361 ttccttccgc gttcacccga taatccttga atgtcttgcc ctcgaccgga tgttgcatcg
     3421 ccaccttcac gccaaagtcg ttatcgttcg ccaccgccag cgttttgctg tcgatcagcg
     3481 ccaacccttc agccttctcc tgctgccagc ccaaggagcg cagatcaaca acctgcgttt
     3541 tctgcgcaag ggtaatgccg cgctgtgaca gggttttctc atcatcaaac tccggatact
     3601 cacccggctt gtcgaagcct gacaggtcgc tcgccttatt gagatcgacc tcgtaaatca
     3661 ggttacgcat tctgttgttt ttatctcttc cctgttcgat cagcaggatg tgctggttat
     3721 cgagcgccac gatgtctccg attttagcgt cgctgttttt gctgtaggcc gcgctgtcga
     3781 taggataacc gtacatcgcg gtttttccgc ttgccggatc gaagctcacc agacgcgtaa
     3841 acagcgcctt tttcttgctc ttagcgtcga tatccagcgt actttgcacg gcgacaataa
     3901 tacgcccgtc cggcatacgg gtgaggcctt caaagccgcg gttcgcctga cgccatttga
     3961 ggatatttgg caaaccgccc gcaatcgctt tttccccttc cgccgcctgc ggaccgtgga
     4021 ttgccaggat tttcccttta ctgtcgatgt taatcaggaa cgggccatac tcatcgcaca
     4081 gccagtaacc tcctttacca tccggagtga tcccttccgt atccagcccg cgattatccc
     4141 ccttcagtct atgtaacgtg tcgctgaagg ccacttcatt ggtggagcca atcacgtcgc
     4201 ttgccagcgg cagaccgttg atcgcgcctt tatcgtcatg cagaggacgg ggatcgatgg
     4261 cctccgcttt gccgttttgt acacgaatcg tcatcagcag cggcgcgaaa tctggcgtaa
     4321 cgaaaatttt agtttcgttt ttcccctctt tcggtgaatc cgcgttcgga ccgcgatcgg
     4381 taatggttgc gaatgtcagg gcatcgccct gtttaccggt gaacagcagg ccagaaccta
     4441 tcccaaccgg aaggccgttc gggaatgcac tggcaaatgc gccggcatag ttcacgtgcg
     4501 tgccttctgg aaaactgacg acatagcgct cagccgtagg ttgtgccgcc agggcagaga
     4561 aagagagcgt acaaccgatt agcacgggaa taatttttct tttcatattg ttagcatcca
     4621 taaagtgaag atcacactac accataaaga agaaacgagt caggaaaatg acatcgaatg
     4681 gcaacatgcg cgggcctgag gccaaagaaa tataaaacac tgttgttatt tgtaacaacg
     4741 gaaacattta cggaaattat ttgtctttct tcgtgatcaa aaatgctgtg tcttgttgga
     4801 ttggagtgat gagggctgcg tttaggaaac gccctatatt gtacggttgc attatcgcgg
     4861 taggggtaat gcacgtgtca gtatgatgag ctgaccacac tcacgtgggt ggactggtat
     4921 aacaatcgac gattgctgga aaggcagggc catattccac cggcagaagc agaaaaagct
     4981 tattatgctt ccatcggaaa cgatgatctg gcagcctgag ttcacagata aaacactctc
     5041 caggaaaccc ggggcggttc aattgctgga taagatcata acgatacacc gggttaaaca
     5101 ggtggtcact gcttcactga agctgcgtga ttatgatggt caggtagcag aagctatgcc
     5161 catgatacca aaatgacgaa agccgaaatg tctgaaagcg tgtgtattac ctgaaaatat
     5221 gaccggctcc ggggtacgta accgattcaa caaagccaat cgatattaca aaacgagagg
     5281 gactatatat ttcgttttca gataagactc cttgagagat tatcttttat gtaaaatctc
     5341 aaatatcacc tgatgtaatg tcaatgataa tattaagtgc gctctgaaca acatctttct
     5401 tggtgtgatt aacatctgac aaaaagaaca tcagcttctt tattatgttc ttgttgttta
     5461 ttatttcacg ctcacagaat aaagcgttga ctgcctcttc tatgacattt ctctcgtaaa
     5521 aaaattgaga gttagcttca tataaaatat tggccaat
//
LOCUS       DAFNBJ010000240         5423 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000240 DAFNBJ010000000
VERSION     DAFNBJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.240"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(944..1135)
                     /locus_tag="L2X49_004632"
     CDS             complement(944..1135)
                     /locus_tag="L2X49_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBN4799604.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGGDGENL"
     gene            1200..1523
                     /locus_tag="L2X49_004633"
     CDS             1200..1523
                     /locus_tag="L2X49_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBN4799605.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRICLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHG
                     ARDDS"
     gene            1501..2223
                     /locus_tag="L2X49_004634"
                     /pseudo
     CDS             1501..2223
                     /locus_tag="L2X49_004634"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2201..2719
                     /locus_tag="L2X49_004635"
     CDS             2201..2719
                     /locus_tag="L2X49_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HBN4799606.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSN
                     ETKIKDAIALFTTSTEESLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVI
                     PYQGKDWVQLTE"
     gene            2726..2959
                     /locus_tag="L2X49_004636"
     CDS             2726..2959
                     /locus_tag="L2X49_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBN4799607.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            3376..3903
                     /locus_tag="L2X49_004637"
     CDS             3376..3903
                     /locus_tag="L2X49_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HBN4799608.1"
                     /translation="MAKEFDVETKGKRTYSMNNDMSDLYNVISRAVEASGADHSINEK
                     LTNVLKRELVDYVSIAHLKTKLSVLYEFEKNYLQLIAEYKEEIKFASSLQEDLRKERA
                     KFFSETLKEVHQTLNESQVDNEVASKWIKELVGSYTKSLDLSGGLVEEHTLDTIACIR
                     AEAKLNKPSIEPGNN"
     gene            3906..4445
                     /locus_tag="L2X49_004638"
     CDS             3906..4445
                     /locus_tag="L2X49_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799609.1"
                     /translation="MAGGRHINSLLYVLVAILERKVNDFKLSSSELPEILAALREARE
                     CLVSRSINEDPQLYSALFLYEKNEGLLSLSFERGYSIFINSNLECILRKVSDIKDKKQ
                     GKLQKIMNEFVRFKYSSSDSCADLLFLSGKLEQIRKIYEVFDRDRNRLEFLNYMMKKE
                     RVIFEREFERLLGEKNGDF"
     gene            4432..5235
                     /locus_tag="L2X49_004639"
     CDS             4432..5235
                     /locus_tag="L2X49_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799610.1"
                     /translation="MGIFSQIGSALLNCISKPVDALCEWAKEPLKDREYRREEASKES
                     EHKRNMDVLTAKKRVEHELRMSELNAEYELREKEKTLEVDLEVRRVREIEKAVAEIQE
                     WRKSQELERMERTTAAIARYKEQLTKLNIEVINSIGNMQLDLKERAQKFVYEKAVQYK
                     RLQDEATEEAMNDLIRIEEKFGDNERAKDILIRAVDTKMGNVIDTSTRFLEELNRDIV
                     SLNQSIDRLTNQGQKFIESHLERFHIVDATSSLYITGDEKERLLLKNIN"
BASE COUNT         1530 a         1038 c         1513 g         1342 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tactgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 gtggctgctg ccagtggcgc tttgtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg tggcctttct cccggccttt gccttttgcg cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
      661 cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagagc gtcggaagct
      721 gcattttctc cttacgcatc tgtgcggcat ttcacacccg gcatggcgca cttttcatac
      781 aatctgcact aatgccgcat ggttaagcca gtatacactc cgctatcgct acgtgactgg
      841 ttcagggctt cgccccgaaa ccccctgacg cgccctgacg ggcttgtctg ctcccggcat
      901 ccgctcacag acaagctgtg accgtctccg ggagctgtat gtgtcagagg ttttcaccgt
      961 cacccccgaa gcgtgcgagg cagctgcggt aaagctcgtc ggcgtggtcg tgaagtgatt
     1021 cacaaatatc ggcctgttca tctgcgtcca gttcgttgag cttctccagc agcgttaatg
     1081 tctggcttct tataaagcgg gccatgttaa gggcggtttt ttcctgttta gtcactgatg
     1141 cctccgtgta aggggggatt tctgttcatg gggtaatgat accgatgaaa cgcgagagga
     1201 tgctcacgat acgggttact gatgatgaac atgcacggtt actggaacgc tgtgagggta
     1261 aacagctggc ggtatggatg cggcggatct gcctgggtga gccggttgcc cgttccggga
     1321 agctgccgac actggcaccg ccgttactgc gccagctggc tgccatcggg aataacctga
     1381 atcagacggc gaggaaggtg aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg
     1441 tggccgcact gatggccatc gagagagagt tgcgcagctt acggcaggtc gtcagggaac
     1501 atggagcgcg ggatgatagt taagtttcat gccaggggaa aaggcggtgg cagtggtccg
     1561 gttgattacc tgctgggaag ggagcgtaac cgggaagggg caacggtgct tcagggtgat
     1621 ccggaagaag tccgggaact catcgatgcc acgccatttg cgaagaaata cacgtcaggt
     1681 gttctgtcgt tcgcggagaa ggagctgccg ccgggagggc gtgaaaaagt gatggcgagc
     1741 tttgagcgtg tactgatgcc cggtctcgaa aaaaatcagt acagcatcct gtgggtggag
     1801 caccaggaca agggacggct tgagctgaat tttgtcattc cgaatatgga gctacagacc
     1861 ggaaaacgcc tccagccgta ctacgaccgg gcagacaggc cgagaattga tgcctggcag
     1921 acactggtga atcaccatta cgggctgcat gaccccaacg ccccggagaa ccgccggata
     1981 ctgactctct ctgataacct gccggaaacg aaacaggcgc ttgctgagag cgtcacacgc
     2041 gggatagacg ctctttatca tgtcggggag ataaaaggcc gtcaggatgt gattcaggcg
     2101 ctcacagagg cggggcttga agtggtcagg gtgacgcgaa gcagtatcag cattgcagac
     2161 ccgaacggtg ggaagaatat caggctgaaa ggagcatttt atgagcaatc ttttacagac
     2221 gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga caatgctgaa
     2281 cgacgagttc agcaagctcg aaaaatctgt aagcaaggct gtgacctcaa acgagacgaa
     2341 aatcaaagac gctatagccc tgttcacaac gtcgacagag gaatcgctgg aaaaacaccg
     2401 ggaaggggtg aaagaggcga tgatgcagca caggaaggac gtgttaaagc tggcagggaa
     2461 tacgggcatg atgttactgg ggatggtcat tttcctgttt accgtgagtg gcgggacgct
     2521 ctggtatctt ggcgggcgga tacaggcgaa cctggaagaa atcaggatac aggaagaaac
     2581 attgcagaaa ctgaacgcga agacatgggg cgtggagttt gtgcaggacg ggagaaggaa
     2641 atttctggtc atcccgcagg ggaaatcggc aacggtgatt ccctatcagg ggaaggactg
     2701 ggtacaactg acggagtgac acagagtgac ggagctggaa agacatttgc tgaacgcatt
     2761 agagcaactg caacaggact atatgcagcg gctgaacgaa tgggagagcg ccttcgtgga
     2821 attgcagaaa atgttttcgc ttacgcaacg ggacaacgcg atgctgaacg agcaggtcat
     2881 gcagttgagt cagcaggtgc agcacttgag cgagcggaca gaacgcttga gccagttata
     2941 cagcgagaac aggagataag ggacgaacgg ctgatacagg aacgcgaaca tgagttatcc
     3001 ctggagcggg aacgccagct ggaaatacag gaacgcacac tggatggtcc ttcactggga
     3061 tggtgaaact atgaaaaatg gcaggttaag tggattaaga cgggctaatg tggtctgtac
     3121 catctggttg cataggtatt catgcggtta aaatttatca ggcgcgatcg cggcagtttt
     3181 tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcgctga acgcgtttta
     3241 gcggtgcgcg taatgcgacg ttatggtaaa tagatgagtt aggcctaagt catgacagat
     3301 aaaacgaccg atattaaaat ggctttagaa aacatgatca gatttcgtga cactatagag
     3361 agattgggag gttctatggc gaaagagttt gatgttgaga ccaaaggaaa acgtacttat
     3421 tctatgaata acgatatgag tgatttatat aatgtaatat cacgggctgt tgaagcgtct
     3481 ggtgccgatc attcaattaa tgaaaaattg acaaatgtct tgaaaagaga attagttgat
     3541 tatgtcagca ttgcgcatct aaaaaccaaa ttgtctgtat tatatgagtt tgaaaagaat
     3601 tatttgcagc ttatcgcaga atataaggaa gaaataaaat ttgcttcctc tttgcaagag
     3661 gatttacgta aagaacgtgc taaattcttt tctgagacat taaaagaggt tcatcaaact
     3721 ctaaatgaat ctcaagttga taatgaagtg gcatcaaaat ggattaaaga acttgttggt
     3781 agttatacca aaagcttgga tctaagcgga ggccttgttg aagagcatac attagataca
     3841 attgcttgta ttcgcgctga ggctaaatta aataaaccat ctattgagcc gggcaataac
     3901 taattatggc cgggggtaga catataaata gtttgcttta tgttcttgtg gctatattag
     3961 aacgtaaagt gaatgacttt aaattgtctt cgtcggaact tcctgagata ttggctgctt
     4021 tacgagaggc aagggaatgc cttgtatcgc gcagcatcaa tgaagatcct cagttatatt
     4081 cggcattatt tttatatgaa aaaaatgaag ggttgctaag tctttcattt gagagaggat
     4141 actctatttt tattaactcc aatttagagt gcatattgag gaaagtttct gatataaaag
     4201 ataaaaagca gggtaaactg caaaaaataa tgaatgaatt tgtgaggttt aaatattcaa
     4261 gcagcgattc ttgtgcggat cttttgtttt tgagtggtaa acttgaacag atacgtaaaa
     4321 tatatgaagt ctttgatagg gatagaaata gacttgaatt tcttaactat atgatgaaga
     4381 aagaacgtgt tatttttgaa agagagtttg aacggctttt aggtgaaaaa aatggggatt
     4441 tttagtcaga ttggtagcgc tttgcttaat tgtattagta agcctgttga tgcactctgt
     4501 gaatgggcta aagaaccatt gaaagatagg gaatatagaa gagaggaggc ttcaaaggag
     4561 tctgaacata agcgcaacat ggatgtcttg acggccaaga aacgtgttga acatgaactc
     4621 agaatgagtg aattgaatgc tgaatatgag ctgagagaaa aagagaaaac tcttgaagtt
     4681 gatctagagg ttcgtcgcgt tcgcgaaata gaaaaagcag tagctgagat ccaggaatgg
     4741 agaaagtcac aggaacttga gaggatggag cgaacaacag cagctattgc acgttacaag
     4801 gagcaattaa cgaaattaaa tatagaagtg attaactcta ttgggaacat gcagcttgat
     4861 ttaaaggagc gcgcacagaa atttgtctat gagaaagctg tacaatataa gcgcctgcaa
     4921 gatgaagcaa ctgaagaggc tatgaatgat cttataagga ttgaggaaaa gtttggtgat
     4981 aatgaaagag ctaaagatat tttaattcgt gcagttgata ctaagatggg gaatgttatt
     5041 gatacgtcaa ctcggttttt ggaagaatta aatagagata ttgttagttt aaaccaaagt
     5101 attgataggc tgactaatca aggacaaaaa ttcattgaaa gtcatttgga gcgttttcat
     5161 attgttgatg cgacatcttc tttatatatt acaggtgatg agaaagaacg attgttgctg
     5221 aaaaatatta attagtattt tgtgagtaat agcgatcatt atttttaatg gtcgcattgt
     5281 ttgttcttgc ggttgctaat cattagtttt tgtcttgtgg ttgaaaactg agcgctatgg
     5341 ctatgacctg ggagtgggag tgaaaagatt caaggatcct cttgtgatcc tttttttctg
     5401 cgcgtaatct gctgcctgta aac
//
LOCUS       DAFNBJ010000241         5260 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000241 DAFNBJ010000000
VERSION     DAFNBJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.241"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..284
                     /locus_tag="L2X49_004640"
     CDS             <1..284
                     /locus_tag="L2X49_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799611.1"
                     /translation="NVVETVYLALSNHARLFGFTAEDIMDFWQHKAPQKYSAFELAFE
                     LGHRVIAELILNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(253..1989)
                     /gene="aceK"
                     /locus_tag="L2X49_004641"
     CDS             complement(253..1989)
                     /gene="aceK"
                     /locus_tag="L2X49_004641"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HBN4799612.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2270..3574)
                     /gene="aceA"
                     /locus_tag="L2X49_004642"
     CDS             complement(2270..3574)
                     /gene="aceA"
                     /locus_tag="L2X49_004642"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HBN4799613.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3604..5205)
                     /gene="aceB"
                     /locus_tag="L2X49_004643"
     CDS             complement(3604..5205)
                     /gene="aceB"
                     /locus_tag="L2X49_004643"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HBN4799614.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISEIASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1220 a         1415 c         1352 g         1273 t
ORIGIN      
        1 aaaatgttgt agagacggtt tatcttgctt tatctaacca tgcacgcctg tttggattta
       61 ccgctgaaga tattatggat ttttggcaac acaaagcccc acaaaaatac tctgcctttg
      121 agttggcttt tgaattgggt caccgggtta ttgctgaatt aatccttaat acattaaata
      181 agatggctga aagctttggc tttacggata accctcgata cattgcggag aaaaattata
      241 tggaagcttt actcaaaaaa gcatctcccc ataccgtacg ctaaatcttt gcctgcgccg
      301 atacgcataa acatcttcca catgcccttc acgtatgcgg ttttgtagtg cgcgccagta
      361 atcagcgcgg aacaggtcgg cgtgcatctc ttcaaacagc ggaccaatac gcgggtcggc
      421 gcatagccag tggcgaaact cttccgggaa aacatcgccc ggcgagacgc tgtaccacgg
      481 ttcgctggca agttcgtctt ccggatagcg cggcggcggg atgtcgcgga aattcacttc
      541 cgtcatgtag caaatttcat cgtaatcata aaaaaccaca cgcccgtgac gggtgacacc
      601 gaagttttta aacagcatgt cgccaggaaa aatgttagca gcggcaagct ggcgaatagc
      661 gttaccgtat tcttcaatgg cgtcgcgcaa ctgctgacct tccacttgtt ccagccagat
      721 attgagcggc accatccgcc gctcgatata gagatggcga attacaatct gctcaccaag
      781 atcggtgatt ttttccgctg cttcctggag cagtaattcc attaatgccg gggaaatatg
      841 ccgcttctcc agcacaaagt tttcaaactc ctgggtgtcc gccattcggc ccacacggtc
      901 gtgctctttc accagttgat agcaggcacg aacgtgagcg gcagacatct ctttctgcgg
      961 cgcgaacttg tctttgatga ctttgaatac ccgatcaaag cccggcagcg taaataccaa
     1021 catcaccatt ccacgaatac ccggcgcttc aatgaactgc tcattacagc cctgtagata
     1081 aacgagatat tcgcggtagc tttcggtttt ggcgtgcttc tggcagccga tagccatata
     1141 caattcagca gtggttttac ccggcagaat ttcccgtagc cactcgacca gcgctgcggg
     1201 cagcggtgca taaaccataa aataagaacg cgcaaagcca aaaacaatgc tcgcttcggc
     1261 ggtcgtcgtc aggcaggtat caataaataa ctcgccgtcg tccgtctggt ggatcggcag
     1321 taagaagggc aacgtgccgg aaggcgtgat cagtttgccc accagccagg cggctttatt
     1381 gcggtaaaac agttcgttcg ccacctgtaa atgactttcc gcgaggttgt ctgtccccag
     1441 cgtttccgtc agatggcgaa tgatgtaatg aatgtcacgg cttttattct gccagcgcag
     1501 gcgcagcggc aggtcgctga taacgcgcat cagtagagat tcccagccgt gatcggggtg
     1561 aaagtctttc gccagcgggc ggggaatggt acgaaagcgg cgctctggct gagagctaaa
     1621 gataaaaagc cgctcgggag taagcgagcg gtggtcaaat aaccgacagt acacggagtt
     1681 aaaaaagctc tccgcaatct cgaagcgcgg gtaatccggc aacagccgcg tgtaatgctc
     1741 tttaacgcgc agtaaaaatg ccgcgtccgt actttggccg ttagtaatgc agcgcagttg
     1801 ctccacgacc agaccaacgt gatgatcgta aagatggata cggtttttca tcgcctgctg
     1861 gacagcatgc cagtcggcct gttcgaaacg ctgctgcgcc ccggaagtca cttcgaggaa
     1921 tcgaccatac tgagcgtcga agccttgcaa aatggtttga gcaatcagta attccaggcc
     1981 acgcggcatt tagcgccctc atcaggagca gagaattgcc ggatgcggcg taaacgcctt
     2041 atccagccta cgttcggcaa cggttgtagg catgataaga cgcgcaagcg tcgcatcagg
     2101 catcgtgcac cgattgccgg atgcggcgta aatgccttat ccggcctacg ttcggcaacg
     2161 gctgtaggca tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg attgccggat
     2221 gcggcgtgaa cgccttatcc ggcctacagt cagcaacggt tgttgttgct tagaactgcg
     2281 attcttcagt ggagccggtc agcgcggtga ctgaagacgt gccgccctga ataatagtcg
     2341 tcactttatc gaagtaacct gtacccactt cctgctggtg agatacgaag gtatagccat
     2401 ctttcgcggc ggcaaattcc ggctgctgca ctttctcaac gtagtgcttc ataccctcgc
     2461 cctgggcata ggcgtttgcc aggtcaaaca tgttgaacca catgctgtgg atacctgcca
     2521 gggtgatgaa ctggaacttg tagcccatat ccgacagctg ctgctggaag ctggcaatag
     2581 ttttgtcgtc gaggtttttc tgccagttga acgacggcga gcagttataa gccagcagtt
     2641 tgcccggata tttcgcgtgg atagcttgtg caaagcgacg cgccagttcc agatccggcg
     2701 tggaggtttc acaccagacc aggtcagcat atggcgcata cgccaggcca cggctgatcg
     2761 cttgctcaat gcccgcatga gtacggaaga agccttcact ggtacgctcg ccagtaataa
     2821 attcactgtc atacgggtcg caatcggagg taatcagatc cgccgcatcg gcatcggtac
     2881 gagcaaccag cagggttgga acgcccgtca cgtcagctgc aagacgcgcc gcgaccagtt
     2941 tctgaatagc ttcctgagtt ggcaccaaaa ctttgccgcc catgtgaccg catttcttca
     3001 ctgacgccag ctgatcttcg aagtgaactg ccgctgcacc ggcttcaatc atcgctttca
     3061 tcagttcaaa ggcattcagg acaccgccaa aaccggcttc cgcatcggca acgatcggca
     3121 ggaagtaatc gacatagcgc ggatcgcccg gctcaatgcc tgcggaccat tggatctgat
     3181 cggcacgacg gaaggtgttg ttgatccgct ccaccacagc tggcaccgag tttgccggat
     3241 agagcgactg atccggatac atgctggccg ccaggttagc gtccgccgct acctgccatc
     3301 ccgacagata gactgcttca atacccgctt tcgcctgttg cagcgcctga ccgccagtca
     3361 gtgcaccgag gctgttgatg tagccttttt tcgactcgcc atgcagcaga cgccacattt
     3421 tcgccgcgcc cagttgcgcc agggtgcatt caggattgac tgaaccgcgt aatttcacca
     3481 catcttccgc gctgtatggg cgagtaatgc cttcccaacg cggttgagtc cactctttct
     3541 gtaattcttc aatttgttgt gtacgggttt tcatgtgcag atgctccata gttatgtggt
     3601 ggtttacgct aacaggcggt agcctggcag ggtcaggaaa tcaattaact catcggaagt
     3661 ggtgatctgt tccatcaagc gtgcggcatc gtcaaaacgc ccctgggaga aacgttcttc
     3721 gcccagttcg ctggcaatga ctttcatctc ttcacccagc atctggcgga acaaggcttt
     3781 ggtcaccggt ttgccattgc tcaacgtttt ttgatgatgg atccactgcc agattgaggt
     3841 acgggaaatt tcagccgtcg ccgcatcttc catcaggcca taaatcggca cgcagccgtt
     3901 gccggagatc catgcttcga tgtactgcac tgccacgcga atgttggcgc gcataccttc
     3961 ttcggtgcgt tcaccatcac aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg
     4021 ttcgcgcatc acttcaagct gatttttacg ggagccgaga atgtcgttga ataccgccat
     4081 tgccgtatcc gcaaggcctg ggtgagcaat ccatgtgcca tcgtgaccgt tattggcttc
     4141 cagcgcttta tccgctttta ctttgttgag cacctggtta ttgcgctctt catctttgct
     4201 cggaataaac gctgccatgc cgcccatcgc aaaagcaccg cgtttatggc aggttttaat
     4261 caacaggcgt gagtaagcat tcaggaatgg tttatccatc gtcactgcct gtctgtctgg
     4321 caggacgcga tcgggatagt ttttcaacgt tttgatatag ctgaagatgt aatcccaacg
     4381 accgcagttc agaccaacaa tatggtcacg cagcgcgtga aggatttcat ccatctggaa
     4441 cacggcgggc agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa
     4501 gcgatcttct gcatagctga agacttcgct ccaccaggcc gcttcctgcc aggactgggt
     4561 tttcggcaga tagaaatagg gaccgctgcc ctttgccaac agtgcctgat agttgtggaa
     4621 gaaatagagc gcaaaatcaa acaggctacc ggggattgcc tcaccacgcc aggtgacatg
     4681 tttttccggc aagtgcagac cgcgtacccg acaaatcaaa accgctggat tgggcttgag
     4741 ctgataaatt ttgcctgctt cattggtata gctgatggtg ccgttaaccg catcgcgcag
     4801 gttaatttgc ccgtcgatca ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc
     4861 cataaagact ttcacattgg cgttcagtgc gttgatcacc atcttgcgct caaccgggcc
     4921 agttatctcg actcgacgat cttgtaagtc cgcgggaata ccacgaattt tccagtccgt
     4981 atcgcgaatg gaagctattt ccgaaataaa atcaggcaaa gttccgttat caatatcttg
     5041 ctgctgctga atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag
     5101 ctcagtcaga aattctaccg cttcggcagt aagaatttgc ttctcctgct cgccatacgg
     5161 ccttatgaaa gccagttcat cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt
     5221 catggatccg aaaacttccc cactgtgaac gaaggatcgt
//
LOCUS       DAFNBJ010000242         5257 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000242 DAFNBJ010000000
VERSION     DAFNBJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.242"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            89..1447
                     /gene="mltD"
                     /locus_tag="L2X49_004644"
     CDS             89..1447
                     /gene="mltD"
                     /locus_tag="L2X49_004644"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBN4799615.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1495..2264)
                     /locus_tag="L2X49_004645"
                     /pseudo
     CDS             complement(1495..2264)
                     /locus_tag="L2X49_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(2342..3142)
                     /locus_tag="L2X49_004646"
     CDS             complement(2342..3142)
                     /locus_tag="L2X49_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBN4799616.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3383..4297
                     /locus_tag="L2X49_004647"
     CDS             3383..4297
                     /locus_tag="L2X49_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4799617.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4294..5097)
                     /gene="dkgB"
                     /locus_tag="L2X49_004648"
     CDS             complement(4294..5097)
                     /gene="dkgB"
                     /locus_tag="L2X49_004648"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBN4799618.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1381 a         1271 c         1307 g         1298 t
ORIGIN      
        1 agaaagatag gttctgataa aactttcttg tcatcggctc cgttcgccgt tatgatcggt
       61 cgtcttttaa gcaactattg acacacacat gaaggcaaaa gcgatattac tcgcctctgt
      121 cctgctcgtg ggctgccaaa gtaccggcaa cgttcaacag cacgcacaga gcctttctgc
      181 agctggtcaa ggggaagcag caaagtttac aagtcaggca cgatggatgg acgatgggac
      241 gtctatcgcg ccagatggtg acttgtgggc tttcattggc gacgagctaa agatgggaat
      301 tccggaaaat gaccggattc gcgaacagaa acagaaatat ttacgcaata agagctatct
      361 ccacgatgta actttacggg cagagccgta tatgtactgg atagccgggc aagttaaaaa
      421 acgtaacatg cctatggaac tggtactact acccatagtg gagagcgctt ttgatcctca
      481 cgcaacgtct ggcgccaatg ccgcaggcat ctggcagatc attccgagca cggggcgcaa
      541 ttatggtttg aaacagaccc gcaattatga cgcgcgtcgc gatgttgttg cttcaacaac
      601 tgccgcgctg aacatgatgc agcgtctgaa caagatgttt gacggcgact ggcttctgac
      661 cgtagcggct tataacagcg gcgaaggtcg ggtcatgaag gcaattaaaa cgaacaaagc
      721 gcgtgggaaa tccacggact tctggtcgtt accgttgccg caggaaacga agcagtacgt
      781 gcctaaaatg ctggcattga gtgatattct caaaaacagc aagcgttatg gcgtacgtct
      841 gccaacgacc gatgaaagcc gtgctctggc gcgtgtgcac ctgagcagcc cggttgaaat
      901 ggcgaaggtt gcagatatgg cggggatttc cgtcagcaag ctgaagacat tcaacgctgg
      961 cgtaaaaggc tccacgctgg gcgcaagtgg cccgcagtac gtgatggtgc caaagaagca
     1021 tgcagatcaa ctgcgtgaat ctctggcttc aggcgaaatt gctgctgtac agtcgacgct
     1081 ggttgccgac aatacgccgc ttaacagccg tgtttacacc gtacgctctg gcgacacgct
     1141 ttcaagtatc gcttcacgtc tcggcgtaag caccaaagat ttgcagcagt ggaacaaact
     1201 gcgcggatct aagctgaagc caggccaaag tttgacgatt ggtgcaggca gtagcgcaca
     1261 gcgactggca aacaacagcg atagcattac gtatcgtgtg cgcaaaggcg attcgctttc
     1321 aagcattgct aaacgccacg gcgtgaacat caaagatgtg atgcgctgga acagcgacac
     1381 tgcgaatctg caaccaggcg ataagctgac gttgtttgtg aaaaacaaca acatgccaga
     1441 ttcctgacaa accagataat aaaaaggcac cgattccccc ggtgcctttt ttatttatgc
     1501 cgctttatgt gcatctacca tgatgatatc actggtgaaa aagccatcat tctgcaaggc
     1561 aaaatacgtt ctcacctctg tcgatgcgct ctgttggtaa atgcgaatag cgtctactaa
     1621 cgcttctggc gtacgcattc tcgcgaccca tgaagaaaat tccagcggta acttatctgt
     1681 aattaaatta tcaactatca gattggcttc attgattaac gtcaaccact caccgctggc
     1741 gtagtttcgt acgtgagaga tatcgcgtaa tgcttctacc gtctgtaacc agatgtcgcg
     1801 cactgggtga cccggagaca ttacgtccat cacaatcagc ctaccgccag gtttcaatat
     1861 cctattcact tctcgcagtg ctgcaccaac atcatgccag tgatgggcag aataacggct
     1921 gataacaata tcaaatgcgt tatcggcaaa tggcagactt tcggcatatc cctggcgggt
     1981 ggtgatattt ttcagttgcc gggcttcggc agcttgtgcc acgacatcca gcatatgggc
     2041 agataagtca tacgccacca ccgtgctcac gttttgcgca gcgacaaagc tggcatgcct
     2101 gctccgcagc ccatatcaag tacacttgca ccaggataat cagccagacg caccgccagg
     2161 cgctgtaaat ctcgtccgga tgcatgtacg gtactggtca gatattcgca ggcttgagag
     2221 ctaaactgct tttctacgtg gtcatggtgg gattgtgttg tcattgcgct gtccttttgt
     2281 tgtttgagta taaaaggaca gcgccaaacg ttggtctgcc ctgtggcaga cctgacatac
     2341 cttatttatc aggcttgccg ggactgaatt caacgagtag cggattgtga tcggaagcgc
     2401 gcgtaaccaa tacagaagct tcgctgacgt tcagaccacg gtagaaaaca aaatcgagcg
     2461 ggcggccaaa cgcccgacgg cgctgatcat cggtaaaacg cacctggcgc agcgacattt
     2521 cccgcgcaaa gcgatataac gcgttcatcc ttctacggct ccaggcattg aaatctcccg
     2581 ccataatgac cgggccgctg tggtgagcta tctgatcacc aataggaagt aactgcttac
     2641 tatagacatc cacgcccaga ctgaagttga cggcgtgtat attaaccacc atcaacaggc
     2701 gggtgtcagg taatggatag accgtcacca gtgccgactt cgccagacgc aaaatgggtt
     2761 ctcgttcacg taacgggcag caatacactg gatgcgccgc cgaaagggtc attacaccgg
     2821 aaggatgttg tggcagcacg aaagcgggca cctgatcggc ggcaagatag ttagcggtcg
     2881 caaactgtac taactctggc gttgtctgcg cttcctgcaa taacaccaga tgtgcatctt
     2941 tgccgtagtt ctttaatacc gacaaccatt cagcgcgttg ctgtttgtat atgttccaca
     3001 ccaggatccg aatacgctct tcggtactta acggttctcc aggtggtaat gcctggccga
     3061 tgctcgcaaa agaccccggc ggtaagatcc tttccgcagg ttgtccggca acatagcgca
     3121 tggcataggt gttttttcgc acttttgact ttgaaacctc tattacgtca acccttccgc
     3181 actcgggagg gttctactgt tatagggatt ttagctcact ctttcaacga gcaattgtat
     3241 attgttatgt aagcaagtgc ttacaccggt cagaattttg ctcattatgg agaacattaa
     3301 atcgcatgac atatcacgca tccagatggt ttaatgacaa ataaattcaa aatatctctt
     3361 ctattttgca acaggagcaa aaatgaaagc cacgtcggaa gaactcgcca tttttgtttc
     3421 ggtcgtcgaa agcggcagct ttagccgggc agcggaacaa ttagggcaag caaactcagc
     3481 ggtaagccgg gcggtgaaaa agctggagat gaaacttggc gttagcctgc ttaatcggac
     3541 cacgcgacaa cttagcctga cggaagaagg cgagcgttat tttcgtcgcg tacagtcaat
     3601 tttgcaggag atggcatcgg cagaatcaga aattatggag acgcgtaata caccgcgtgg
     3661 actgttacgg atcgatgccg caactccagt ggtgctgcac tttctgatgc cgttaattaa
     3721 gcctttccgt gaacgctatc cggaagtcac tttgtcgcta gtctcctccg aaacgattat
     3781 taatttgatc gaaagaaaag tggatgtcgc gatacgcgct ggtacgttaa cggattccag
     3841 cttacgtgcc aggccgttat ttaacagtta tcgaaaaatt atcgcctccc ccgattatat
     3901 ttcccgctac gggaagccag aaacgatcga cgatttaaag caacatattt gcctgggatt
     3961 cactgaaccc gcttccctca atacctggcc gatagcccgt agcgatggac aattacatga
     4021 ggtgaagtac ggtttgtcat ccaatagtgg ggaaacactg aaacagcttt gcctgagtgg
     4081 caacgggatt gcgtgtttgt ccgactacat gatcgacaaa gaaatcgctc gcggagaatt
     4141 ggtggagtta atggcagata aagtgttgcc agtggaaatg ccattcagtg ccgtctatta
     4201 cagcgaccgt gcggtaagta cgcgcatccg ggcttttatc gatttcctta gcgagcatgt
     4261 aaaaacagct cccggaggag ctgtcagaga ggcttaatcc cattcaggag ccagaccttc
     4321 cgggctaacc aggcggtcgt tgcaatccag tgcggcgatc gcttttttat cttcggcatc
     4381 aagctgtaaa ttttgtgcct taagattact ttccaggttt ttacgtttag tagaagaagg
     4441 aattactgag taaccttccc ccatagccca cgccagaatc acttgtgccg gagtcgcatt
     4501 gtgtttagct gcgatacgag caataacctc atctttcagg gccttaccat acgccagcgt
     4561 catataggaa gtaatatgga tgccgtgctg tttagcccag gcaaccactt tacggttttg
     4621 cagataagga gagagttcaa tctggttagt agcgatgttt tcagcaccaa cagcagcaat
     4681 cgctttttcc atcaacggga tcgtgaagtt ggaaataccg atctcacgcg tcagcccttg
     4741 ttttttggct tccagcagcg cctgcataaa ctcttcaaca gagacttcat cgtttggtga
     4801 cggccagtgg attagcgtca gatcaacata atcggtacgc aatttttgca ggctctcttt
     4861 cagacttggg atcaatttgt ctttgctgag attttcaatc cagattttag tggtgatgta
     4921 gagttcatga cgtggcacgc cactttctgc aatcgcctga cctactgcgg cttcgttatc
     4981 atagatttgt gcggtatcaa ttgcgcgata accaagttca agcgccgtta tcacagatga
     5041 aataacaacg tcgtctttca gacggaaagt acctaaacca aatgcaggga tagccataat
     5101 atgcctcttt taatgctatt ttcgttaact gaggtgatta tgggcttcag actgttgagg
     5161 aaaaagagcg taaaatgcag aggatttttg cgattctggc aataatagat atacagacga
     5221 aaaaaaacct cgagcgttaa ctcgaggctt cttaata
//
LOCUS       DAFNBJ010000243         5252 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000243 DAFNBJ010000000
VERSION     DAFNBJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.243"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(66..359)
                     /gene="cas2"
                     /locus_tag="L2X49_004649"
     CDS             complement(66..359)
                     /gene="cas2"
                     /locus_tag="L2X49_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HBN4799619.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(356..1279)
                     /gene="cas1e"
                     /locus_tag="L2X49_004650"
     CDS             complement(356..1279)
                     /gene="cas1e"
                     /locus_tag="L2X49_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HBN4799620.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(1276..1926)
                     /gene="cas6e"
                     /locus_tag="L2X49_004651"
     CDS             complement(1276..1926)
                     /gene="cas6e"
                     /locus_tag="L2X49_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBN4799621.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELRTGQQLCFNLRANPTICKS
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(1908..2654)
                     /gene="cas5e"
                     /locus_tag="L2X49_004652"
     CDS             complement(1908..2654)
                     /gene="cas5e"
                     /locus_tag="L2X49_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HBN4799622.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(2665..3549)
                     /gene="cas7e"
                     /locus_tag="L2X49_004653"
                     /pseudo
     CDS             complement(2665..3549)
                     /gene="cas7e"
                     /locus_tag="L2X49_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            complement(3548..5252)
                     /gene="cas3"
                     /locus_tag="L2X49_004654"
                     /pseudo
     CDS             complement(3548..5252)
                     /gene="cas3"
                     /locus_tag="L2X49_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
BASE COUNT         1254 a         1461 c         1247 g         1290 t
ORIGIN      
        1 ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt taaagaacgt
       61 ctaacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc caaatccacc
      121 ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcggt attggtcgcc
      181 caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca gatcatctcc
      241 cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag tagccagatt
      301 gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac catgctcatc
      361 cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg gcaggcggta
      421 acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact tcctcaatta
      481 acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag gctaatctga
      541 cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt ggcacaaccg
      601 aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg ccactatgga
      661 taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat ataccgtaca
      721 gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc cagtctttag
      781 gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat gcatacgtct
      841 ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga gctggcggtg
      901 gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga tcttccgtta
      961 aagccagctt tgcctggtag agtaatttat ctgcccgcgc ccctccgggt tgtccggagg
     1021 agtaaacgcg aacgcccgct tcaccgaccc agaccagtag cgtccccacc gtggcggcta
     1081 gatgcaccgc cgcgtgggaa actctcgttc ccggttcgag cataatgcag gcgaccgatc
     1141 ccaccggaat gtgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg ccgtccagta
     1201 cgtcgatctg accgtactgg aggaagatca tggaggtgcg atcttttaac gggatcggac
     1261 tcagtggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac cacagccgaa
     1321 cgcccggctt ttgccatatc cctggctaag acgttgcaga aataacccgg gatcggtgac
     1381 cgtaagcatc cccgtataat ccacactact gaactggatc agttgccggg agttttcccg
     1441 ccggagttgc tgttgtcggt aggcatcaac cgaggtatcc agtagtgtaa aaccacttct
     1501 ttccccctgc gctgccagcc agtccagcgc cgcctgttgt tgatgcaacc agacatcact
     1561 tccttccgcc tgtcccctca cctgccgttt cgcctccatc agcagatcgt ggcgcttgcc
     1621 cgatttacag attgttggat tcgcccgcag gttgaaacaa agttgttgtc cggtacgcag
     1681 ctcgggcgca aatgaccggc attctatggt gaacgtatca ctttccgccg ggcgttcctg
     1741 cgagaggaca aaaaagcgaa acgcgccctg gagttcttcc cgacgataaa gaaattgcct
     1801 ttctttgccg ccagggaaaa gatcccacag ccactgatgc atcacatatt caccgcgatc
     1861 caccagatgc agcaactgcg caggcgaaag ctggccggta tgtaaggtta ttcttgagag
     1921 gtacatggtt cctccttgct gagccacggc ccctgattga tggtgcgttc cccaaacaac
     1981 cactgctgac gatttaaagg aacatcccga cggcgtaata ttttgctcgc gaccaggccg
     2041 tcgtgttccc cttcccacca gcattcatcc tgaagtttcg ggagtgagac tttcagttta
     2101 tggaaatgat cctgatactg ttggtatgca ttacataagg catcacacgc gttgccttcg
     2161 agcagtaacg gcgcaagagg taatgccagc ggatgacttt ttcgccccag ataaagcgga
     2221 aaaactggat ggcgtaaacc gtcctgcaac tgttcgaggc tgtaaggcgc atcggctgtt
     2281 gttgccaccg ccaccatcca ccaggcatcg gtgtagtagt cgcgccggga gataatcgcg
     2341 ctcagaagat cagggtcgct caactcttcg cgacggctga aataacgcgc tttacgcacc
     2401 tcttttggca tctggatagt gtgataatcc cgtgcccagc gcgggttacg gctggcgcaa
     2461 accaccagtg aatagtggcg attaaacgcg tttaagcgtt cggtatcatc acgccgaatc
     2521 cctaccccgg cagccagcag ccccagcaat gctgagcgcg aaggcagttc atgggtatga
     2581 cgcacttcgc cgggggcatc gacgccccag gatgccattg gcccatgaag ctgaaaaatc
     2641 agatattggc tcataagccg ccccttacgc gcagataaag tccagcacgt ccttcatgct
     2701 tccctgtttg ttcatcacgt caaaacttgc gcattcggtc ttctgttcgt agaccgtatt
     2761 catgttttcg cgaagcgttg taatacgctg caccgccacc tctaactgcc gggtgccatt
     2821 aatgggttca tagaaagccg ccgccagaga acgcggttgt tcggtacctt tttctgccat
     2881 cgcccaggag gcgtaggcac ggctggcaaa gctgttctgt ttgccggttg gggagacttt
     2941 aagcgcggct tccgtaaagg cgcgtatggt ctgattagct aacgcttcgt caccgccgag
     3001 gttttcgacc agcagatctt tatcaatgca gatataggtg tagaacagtg cagaaccgaa
     3061 tccggtttca ccaagatgcc cggcaccggc atcttcagaa gcctggcgca aatcatcaac
     3121 ggcggtgaaa aaatcatctt cgacaatcgt ttcactgaca ccaaatgcgt gcgctacctg
     3181 gcaggcggct tcaacattaa actcgggttt attcgccagc atacgaccaa acatggcaat
     3241 atctaccgcc atacggtctt tgcgtaacaa cgcgagatct tcctcttttg gcgcgcgctt
     3301 ttcttctgca agctgatggg ccagcgcttt tacggcgtca aattctgccg ggctgatatg
     3361 aactaattgt tcagtttcgg cgttagtgag cggatctttt ggttttttgt cgtttttagc
     3421 tttcccaaga taatccgcaa tttttgccgc ccattcgatg gcttttttct cttcgattcc
     3481 tttctcaatc aggatagttg ccgcctcacg cgcaatacgc ccactacgaa taccaatatg
     3541 gcccgccaag taactgacga taaaccttaa tagcgtcatc gacagaattc cggatccagg
     3601 cgatgcattt cccctgactt accgctgatt cgatacgcgc aatacattct tgttcgctat
     3661 gaagccaacc cacgctcacg ctacgctcaa cttctttgcg cgtcgctacc cggtgcgaat
     3721 gtacatcggt tttcgtgaca tgcgtcagcc aggggtaatc atcttttccc aggagtgggg
     3781 cttcttgctg gccctctgca ccacgcgcaa aggcggcgac gagtttgtcg cgctgctgtt
     3841 gagataacgt agcagaaagc aaaataacgc tgtttccgcc acgcgcctgc cgctcgatca
     3901 gcccttcaag aatgcacgac atgtaagcgt cacaggcgtg gatctcatca gccagcagga
     3961 ttttgttact cagccccaga agccgcagat tattatgttt gaacggcatc accgccatca
     4021 tcgcctgatc cagcgtgccg acgccaattt cagccagcaa cgccttcttg ttactgtcgg
     4081 caaaccaggc cgcacatccc tgactgaacg tttgttcatc cggttcttct gacccgacta
     4141 aatcaccgga ccagagtgat tcattaaagc ggtccattaa tgtgcgggca ctatgtgcca
     4201 gcaccaggct ggggcgggat tctggcgaat agaaagcaag ccaggttttg accagccgat
     4261 cgtacatggc attggccgtt gccattgttg gcagaccaaa aaacagcccc tgagctttcc
     4321 ccgcagctat caacctgtgc gccaggataa gcgccgcttc tgttttacct gcgccagtca
     4381 catcttccag aataaataac tgcggtcctg gctggctgat atccagatcc agcacctttt
     4441 gctgtaacgg tgtcggattc tcaataaaag gaaacagtgt attaattccg gtgaaaggtg
     4501 cggtttctgc ttttggagga aagacggtta atgcgttttg agcctgcact aaagccttct
     4561 gccagtaatg tttaatatcc attgcctgtg cgacgcgtgg aaaaaatcgc gttgacgaac
     4621 ctgtccagtc tgcgagtacg acggttgcag agatatacca ggaaaggtgt tttataagtt
     4681 caatgccctc gtcatcatcc cagaatgcgg ggatctctat tcgcggaaac agtgccttga
     4741 ttgcaaggag aaaatctcgt gctgcagctt tgtcttcagg cagaaaatta tccaattcat
     4801 ccatacgatc agggggccga ccatgatgcc ctgtcgtcac gggcatccac atttctatca
     4861 ctcgcctaag tttgcgcgga gagagcgaag atgaaggaag taactcctga cattcactta
     4921 aataatgatt ccacaaccag tagcccagcg ttgaatgaga gattttttcg taattttttc
     4981 tggcaccttc ctgaatcttg agttcagggg gcaggtaaag ttgttgaaaa gagcgggcaa
     5041 attttccaat atcgtgccag cacagcaacc aggcgaaaaa ttgagccgcc tgttccttgt
     5101 cagaaatccc taattgacga aaataatcag ccagcccgaa gcaatttctt ttaaccatta
     5161 aatagcccat cgcggccaca tccagcgaat gccagcaaag aaggtgatag tcgtcgcccc
     5221 cttctttctc gccgcgtcgg gtttttcccc ag
//
LOCUS       DAFNBJ010000244         5175 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000244 DAFNBJ010000000
VERSION     DAFNBJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.244"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..1945
                     /gene="acnA"
                     /locus_tag="L2X49_004655"
     CDS             <1..1945
                     /gene="acnA"
                     /locus_tag="L2X49_004655"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBN4799623.1"
                     /translation="LIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVEFY
                     GDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYAKA
                     QGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELEVNAT
                     HKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLKRQ
                     PWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIETAIK
                     KGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEPIGHDRK
                     GDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYGWQED
                     STYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRYLQ
                     GRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVSIYD
                     AAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMG
                     ILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCRCRID
                     TATELTYYQNDGILHYVIRNMLK"
     gene            complement(2009..2599)
                     /gene="ribA"
                     /locus_tag="L2X49_004656"
     CDS             complement(2009..2599)
                     /gene="ribA"
                     /locus_tag="L2X49_004656"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBN4799624.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2769..3533
                     /gene="pgpB"
                     /locus_tag="L2X49_004657"
     CDS             2769..3533
                     /gene="pgpB"
                     /locus_tag="L2X49_004657"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBN4799625.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3682..3990
                     /gene="lapA"
                     /locus_tag="L2X49_004658"
     CDS             3682..3990
                     /gene="lapA"
                     /locus_tag="L2X49_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBN4799626.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            3997..5166
                     /gene="lapB"
                     /locus_tag="L2X49_004659"
     CDS             3997..5166
                     /gene="lapB"
                     /locus_tag="L2X49_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBN4799627.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
BASE COUNT         1269 a         1223 c         1402 g         1281 t
ORIGIN      
        1 gcttatcccg gacgtagtgg gctttaaact taccggtaaa ttacgtgaag gtattaccgc
       61 cacagacctg gttctcactg ttacccaaat gctgcgcaaa catggcgtgg tggggaaatt
      121 cgtcgaattt tatggtgatg gtctggattc actaccgctg gcggatcgcg ccaccattgc
      181 caatatgtcg ccagaatatg gtgccacctg tggcttcttc ccaatcgatg ctgtaaccct
      241 cgattacatg cgtttaagcg ggcgcagcga agatcaggtt gagttggtcg aaaaatatgc
      301 caaagcgcag ggcatgtggc gtaacccggg cgatgaaccg atttttacca gtacgttaga
      361 actggatatg aatgacgttg aagcgagcct ggcagggcca aaacgcccac aggatcgcgt
      421 tgcactgccc gatgtaccaa aagcatttgc cgccagtaac gaactggaag tgaatgccac
      481 gcataaagat cgccagccgg tcgattatgt tatgaacgga catcagtatc agttacctga
      541 tggcgctgtg gtcattgctg cgataacctc gtgcaccaac acctctaacc caagtgtgct
      601 gatggccgca ggcttgctgg cgaaaaaagc cgtaactctg ggcctcaagc ggcaaccgtg
      661 ggtcaaagcg tcgctggcac cgggttcgaa agtcgtttct gattatctgg caaaagcgaa
      721 actgacaccg tatctcgacg aactggggtt taaccttgtg ggatacggtt gtaccacctg
      781 tattggtaac tctgggccgc tgcccgatcc tatcgaaacg gcaatcaaaa aaggcgattt
      841 aaccgtcggt gcggtgctgt ccggcaaccg taactttgaa ggccgtatcc atccgctggt
      901 taaaactaac tggctggcct cgccgccgct ggtggttgcc tatgcgctgg cgggaaatat
      961 gaatatcaac ctggctgctg agcctattgg tcatgatcgt aaaggcgacc cggtttatct
     1021 gaaagatatc tggccatcgg cacaagaaat tgcccgtgcg gtagaacaag tctccacaga
     1081 aatgttccgc aaagagtacg cagaagtttt tgaaggcaca gcagagtgga aggaaattaa
     1141 cgtcacacga tccgatacct acggttggca ggaggactca acctatattc gcttatcgcc
     1201 tttctttgat gaaatgcagg caacaccagc accagtggaa gatattcacg gtgcgcggat
     1261 cctcgcaatg ctgggggatt cagtcaccac tgaccatatc tctccggcgg gcagtattaa
     1321 gcccgacagc ccagcgggtc gatatctaca aggtcggggt gttgagcgaa aagactttaa
     1381 ctcctacggt tcgcggcgtg gtaaccatga agtgatgatg cgcggcacct tcgccaatat
     1441 tcgcatccgt aatgaaatgg tgcctggcgt tgaagggggg atgacgcggc atttacctga
     1501 cagcgacgta gtctctattt atgatgctgc gatgcgctat aagcaggaac aaacgccgct
     1561 ggcggtgatt gccgggaaag agtatggatc aggctcaagc cgtgactggg cggcaaaagg
     1621 tcctcgtctg cttggaattc gtgtggtgat tgccgaatcg tttgaacgaa ttcaccgttc
     1681 gaatttaatt ggcatgggca tcctgccgct ggaatttccg caaggcgtaa cgcgtaaaac
     1741 gttaggacta accggggaag agaagattga tattggcgat ctgcaaaatc tacaacccgg
     1801 tgcgacggtt ccggtgacgc ttacgcgcgc ggatggtagc caggaggtcg taccctgccg
     1861 ttgtcgtatc gacaccgcga cggagttgac ctactaccag aacgacggca ttttgcatta
     1921 tgtcattcgt aatatgttga agtaacaagt atttgcttgc cggttatttt gcttccggca
     1981 agcaaactaa ttacacaatg caagagggtt atttgttcag caaatggccc attttctcgg
     2041 ctttggtatc gagataatgt tcgttattgg ggttacgacc tacaatcaat ggtacgcgtt
     2101 caacaatatt aatccctgct tcggtcagaa tttcgacttt tttcgggtta ttggttaaca
     2161 agcggacttc attgacgcca aggagtttga acatatcagc gcaaagagtg aagtcgcgct
     2221 catcagcggc gaagcctaac tggtggttag cctctacggt atcgtaacct tgatcctgca
     2281 gtgcgtaagc gcggatttta ttcagcagac caatgttacg accttcctga cggtgataca
     2341 gcaaaatacc acggccttcc tcggcaattt gcgtcaatgc cgcttcgagc tggaagccac
     2401 aatcgcagcg caagctgaac agggcgtcac cggtcagaca ttcggaatgg acgcgcgcaa
     2461 gtaccggggt atgcccggaa atatcgccat agactagcgc gacatgatcg tgtccggttg
     2521 ccagttcttc aaatcccacc atcaggaaat cgccccatgg ggttggcagt ttggcttctg
     2581 ccacacgttt aagctgcatg aaattctcca gataatgctg gttctgtatt ggcttatttt
     2641 gccataacga gaaggggttc acctaatcac gacgcgtcga tcgttcacgg aatggcacaa
     2701 atctgtcaat ttttcctgga actggcgttt tcagttatga ttgtgggact tatcaaaaag
     2761 gagaggccat gcgttcgatt gccagacgta ccgcagtggg agctgcacta ttgcttgtca
     2821 tgccagtagc cgtatggatt tctggctggc gttggcaacc tggagaacaa agttggctac
     2881 taaaagcggc tttttgggtt actgaaactg tcacccagcc ctggggcgtc attacacatt
     2941 tgattttatt cggctggttt ctctggtgtc tgcgttttcg cattaaggct gcctttgtat
     3001 tatttgccat tctggcggcc gcaatccttg tgggacaagg cgttaaatcc tggatcaaag
     3061 acaaagtcca ggaaccacga ccttttgtta tctggctgga aaaaacacat catattccgg
     3121 ttgatgagtt ctacacttta aagcgagcag aacgcggaaa tttagtgaaa gaacagttgg
     3181 ctgaagagaa aaatatccca caatatttgc gttcacactg gcagaaagag acggggtttg
     3241 cctttccttc cggtcacacg atgtttgctg ccagttgggc actgctggcc gttggtttgc
     3301 tgtggccgcg tcggcgaacg ttaaccattg ctatcttgct ggtctgggca acgggagtca
     3361 tgggaagccg cctgctgctc gggatgcatt ggccacgcga tctggtagta gctacgttga
     3421 tttcgtgggc gctggtggcg gtggcaacgt ggcttgcgca acgaatttgt gggccattaa
     3481 caccacctgc ggaagaaaat cgcgaaatag cgcaacgaga acaagaaagt taacgctggt
     3541 tgattttccg aatttagccc ttaaatcatc aacaatgcgc gtggatgcca tttcgcagac
     3601 ggcgcgaaaa tggtacttta aagggctatt gcggtaagtt gaccataatt tattcgctct
     3661 aaccacataa cgggaagtaa tgtgaaatat ttactcattt tcttactggt gttagcgatc
     3721 ttcgtgattt cggtcacgtt gggtgcgcag aacgatcaac aggtgacgtt taattatctg
     3781 ttagcgcaag gggagtaccg tatttccaca ttgctggcgg tattgtttgc tgcagggttt
     3841 gctatcggtt ggttgatttg tggcctgttc tggctgcgag ttcgtgtttc cctggcgcgc
     3901 gctgaacgta aaataaagcg actggaaaac cagctttcac ccgcgactga cgtggctgta
     3961 gtgccgcact cgtcagcggc gaaggaataa ctttctatgc tggagttgtt gtttctgctt
     4021 ttgcctgtag ccgctgccta tggctggtat atgggccgca gaagtgcgca acaaaacaag
     4081 caagatgaag ccaaccgctt gtcgcgtgat tacgtagcgg gggttaactt cctgcttagt
     4141 aatcaacagg ataaagcggt agacctgttt ctcgatatgc ttaaagagga tacaggcacc
     4201 gttgaagccc accttacgct cggaaacctg ttccgttcgc gtggcgaagt tgatcgcgct
     4261 attcgcatcc atcagaccct aatggaaagc gcctcgctga cctatgaaca gcgtctgttg
     4321 gcgattcaac aactagggcg tgattacatg gccgccgggt tatacgaccg cgcggaagac
     4381 atgttcaatc agctgaccga tgaaactgac ttccgcattg gcgctctgca acagttgcta
     4441 caaatctacc aggctaccag tgagtggcag aaagcaattg atgttgccga acgcctggtg
     4501 aagctgggta aagataaaca gcgcgtcgaa attgcccatt tctactgtga gttagccctg
     4561 cagcatatgg ccagcgacga tctcgatcgt gccatgacct tgctaaaaaa aggggcggcg
     4621 gcagataaaa acagcgcccg cgtatccatc atgatgggac gcgtgtttat ggcgaaagga
     4681 gaatacgcca aagccgtcga aagtttgcaa cgcgtgatat cccaggacag agaactggtc
     4741 agcgaaacgc tggaaatgtt gcaaacctgc tatcagcagt tgggtaaaac tgccgaatgg
     4801 gcagaattcc tgcaacgcgc ggtggaagag aacaccggtg ccgatgctga attgatgctt
     4861 gctgatatca tcgaagcgcg cgacggtagt gaggccgcac aggtctatat tacgcgccag
     4921 cttcagcgtc atccgaccat gcgtgtgttc cataagttaa tggattacca cttaaatgaa
     4981 gcggaagaag ggcgtgccaa agagagcctg atggtgctgc gtgacatggt tggcgaaaag
     5041 gtacgtagta agcctcgtta tcgctgccag aaatgtggtt ttaccgcata caccctctac
     5101 tggcattgtc cgtcttgtcg ggcctggtca accattaaac cgattcgcgg tcttgatggc
     5161 ctgtaatttt taaaa
//
LOCUS       DAFNBJ010000245         5132 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.245, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000245 DAFNBJ010000000
VERSION     DAFNBJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.245"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(64..1083)
                     /locus_tag="L2X49_004660"
     CDS             complement(64..1083)
                     /locus_tag="L2X49_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HBN4799628.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(1095..2309)
                     /gene="rspA"
                     /locus_tag="L2X49_004661"
     CDS             complement(1095..2309)
                     /gene="rspA"
                     /locus_tag="L2X49_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBN4799629.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2515..2841)
                     /locus_tag="L2X49_004662"
     CDS             complement(2515..2841)
                     /locus_tag="L2X49_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBN4799630.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2976..3317
                     /locus_tag="L2X49_004663"
     CDS             2976..3317
                     /locus_tag="L2X49_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBN4799631.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3352..3912
                     /gene="speG"
                     /locus_tag="L2X49_004664"
     CDS             3352..3912
                     /gene="speG"
                     /locus_tag="L2X49_004664"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HBN4799632.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3915..4625)
                     /locus_tag="L2X49_004665"
     CDS             complement(3915..4625)
                     /locus_tag="L2X49_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBN4799633.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4733..5038
                     /locus_tag="L2X49_004666"
     CDS             4733..5038
                     /locus_tag="L2X49_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBN4799634.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1397 a         1295 c         1121 g         1319 t
ORIGIN      
        1 tgtcataatg gttatctcta aaaaagaggt aagatgcgta ctacttactc gccgttattg
       61 gtattattca gaaaaagtga gtaagacttt gcagcaatgt ttttgatcct gttcaaataa
      121 actaatggca tcagcaacat gctggaaatc aaacgtatgg gtaattaatt tttctggttt
      181 aattaaccct ttacttaacc agtcgataac aaccggaaat ttatttgcat ttaagcgtga
      241 agagaaaata gagagttctt ttccggtaat tccttgctga atcacttcag acggttcact
      301 ggagaagccc atcaatacaa tacgtgccgc tggagaagcc agcgttacgg cctctttcag
      361 gatagaagga tgacaagccg catcgataat taatgtcggc ttgatgcctt tttcagcgaa
      421 aatctcgcca agcggtgtct ggctgttatt gatcgcccag tctgccccgc tctctttcgc
      481 tttttccagt cgttcatcaa tgcgatcggc aacaatcaca tttttaacgt tatagacgcc
      541 ttttaatacc tgaacgatcg tcaggccgat tggaccggca ccataaacca gaacggtatc
      601 attttcagtc ggttgaccat gtccggttac gttagccgca atggtaaaag gttcgatcat
      661 taccgcatat tgatcggcca ctgcttcagg aattttccac gcattttttg ccggaaccac
      721 ggcatattca ctgaaaccac cgtcagcgtg cacacctaaa acagccagtg tcgtacaaac
      781 gttcggttta cctatagagc acggatagca atgcccacag ctgaccaccg gatcgacagc
      841 aacacgttca ccgactctgg cgctttccac gccgtcaccc accgcatcaa tgacgccaaa
      901 gaattcatgt ccaatgacgc gcggatattt cgcaaaagga ttatgcccac gataaatatg
      961 gctatctgaa ccacaaattc cggcaagttt cacttttact cgtacttcac ccgctgacgg
     1021 ggtgggtatt tcacgttcga caatcgccag ttgattcggt ttttcaatta atatgctttt
     1081 cattatctta ctccttacca gttccacagc gtgccatctt ccagacgtgc gactggtaga
     1141 taagcaggtt catagggata tttcgccgcc agcttttcat cgaattcgat gccaagaccc
     1201 ggtttgtctc ccggatgcat atagccgtta tcgaaagtcc agttgtgcgg gaagacttcg
     1261 agcatttgtt cggaataacc catgtattcc tggacaccga aattggggac ccacagatca
     1321 aagtgcagcg ccgcagccat gcagactggt gacaaatcgg aaggaccgtg tgagccagta
     1381 cgtacctgat acagcgaagc aaaatcggca atccggcgca taccggtaat tccgcctgca
     1441 tgggtcagcg tggtgcggat ataatcgatg agttgctctt caatcagttg tttgcagtcc
     1501 cagatgctgt tgaagacttc acccactgcg atgggtgtga cggtatgttg gcgaatgaga
     1561 cggaagcatt cctggttttc tgcaggcgtc gggtcttcca tccagaacat gcgataatct
     1621 tcaatgcttt taccaaagcg cgccgcttca ataggcgtta agcgatggtg catgtcatgc
     1681 agcaaatgtt cattaaaacc aaacttgtta cgtaccgcgt caaacaattt cggcatgaaa
     1741 tcgaggtatt tctccgtcga ccacagctgc tcttccggcc actgtccttt ggttgcgggt
     1801 tcataagcca gacctttacc tttcgacatg ccgtaggtgg ttttcatacc agggattccg
     1861 cactgcacgc ggatggcttt gaatcccagc tcctgatgac gggcataatc atccagagct
     1921 tcatcaatac tgtgaccggt ggtatggcaa taaaccatca ccccttcacg agacgcgccg
     1981 ccgagtaact ggtaaagcgg catgttggca gctttggctt taatatccca cagcgccata
     2041 tcaaccgctg aaatggccga catcgtaacc ggaccgcgac gccagtaagc acctttatag
     2101 aaaaactgcc agatatcttc gatacggtgc gcatcgcgac caataagctg cggacaaagg
     2161 tgatcctgca aataagaggc cacggaaagc tcacgtccat tgagggtggc atccccaagg
     2221 cccgtaatac cgtcctcagt ggtgattttt aatgtgacga aattacgccc cggacaggta
     2281 acaaaaactt cagcctttac gatcttcatg ttcgattcct tgcatcgctt gtcgtgatgc
     2341 atgaaatcta cgcaactgag ctactaccat acaagtataa agatcgaaaa aagccggagt
     2401 gatcacaaaa aaaggcgtat atttgcgctg tgaatggttg acaaaagatg aaatagaata
     2461 ccttttgtca gctgacactt cctcttatct tattgataaa atggatttat gttcctacgt
     2521 gcgcccccag cccgcaacaa tgatcaacat gccgcaaagc gcaatcaacg cccccgtcca
     2581 gtcataaaga gtcagtttca cgccatccac aacgcgcagc cacatcaacg ccgtgcagac
     2641 ataaacgcca ccataagccg cgtaaacacg cccactcgcc gctggatgca acgttaacaa
     2701 ccagacaaac agcgccagtg aaatccccgc cggaagcaac agccagatac tggcgtttcg
     2761 ttttaaccac aaccagggca gaaagcatcc aataatttca cacagcgcag tagcaaaaaa
     2821 tagtaacgtt gttttaatca tctttgtctc ttattgacat catgtatagt tatagggcga
     2881 cataatatca tcaatataaa caccctcctg gtacgttttg cgtccgcagt ggatggtgta
     2941 gaatcacctt taatcattca tacagggaat gaattatgaa aatcactctc agcaaacgaa
     3001 tcggcctgct cgctattctg ctgccttgcg cactggcatt gagcacaact gttcatgccg
     3061 aaactaacaa actggtgatt gagtctggcg acagtgcaca aagccgccag cacgccgcta
     3121 tggaaaaaga gcaatggaat gacacgcgca atctgcgcca gaaagtgaat aaacgcactg
     3181 aaaaagagtg ggataaagcc gacgccgctt ttgataaccg cgataaatgt gagcaaagcg
     3241 ccaacatcaa tgcctactgg gagcccaata ctttgcgctg cctggaccgt cgaactggcc
     3301 gcgttattac cccctaacct gttattgatt taaggaatgt aaggacacgt tatgccaagc
     3361 gcccacagtg ttaagctacg cccgctggag cgtgaagatt tacgctatgt acatcaactc
     3421 gacaacaacg ccagtgtgat gcgttactgg tttgaggaac cctacgaagc ctttgttgaa
     3481 ctctctgatc tgtatgataa gcatattcac gatcagagcg aacggcgctt tgtggtggaa
     3541 tgtgacggcg aaaaagccgg tctggtggag ctggtggaaa ttaaccatgt tcatcgccgc
     3601 gcagaatttc agataattat ctccccggag tatcagggga aaggtctggc aacccgtgcc
     3661 gccaaattag caatggacta tggctttacc gttctcaatc tctataagct gtatctgatc
     3721 gttgataaag agaatgaaaa agcgattcac atttaccgca agcttggctt ttcggttgaa
     3781 ggtgaattga tgcacgagtt ctttattaat ggtcaatatc gtaatgccat tcgcatgtgt
     3841 atattccagc atcagtatct ggcagagcac aaaacaccgg gtcagactct cctgaagccg
     3901 accgcacaat agcattaata ataatcgatc gtatttttga tggtgtaaac ccgttcgacg
     3961 gcgggtttta ctccttcatc aacaatgatt agctgacagt ccaccggatt agcgtgactg
     4021 tcatattcac agctctgttt tacattacca acccgttgat tattcagtaa agtaaccgct
     4081 gtgtaatcta attttttgat cggatccgtt gatggcgtgg cgctgacaga taatgttttg
     4141 tcgttacttt tcgtggtttt acccagcgga taaccctgat catcatagcg atattccatc
     4201 tgcatttgtt tgctgctggc tttaatcacg aagccattat catcggtttc ccaactcacc
     4261 ccggcagaag gtaattctgc tagctggcat tttccctgta aacgtactct cttctccagc
     4321 gtctcggcat cacggtaata attggcgtcc agtaccagag cgaccacggt attattttcc
     4381 agatccagta attcgagtga atcaaaacag ccttcttccg acaaagtccc agaaacacgt
     4441 ttcgtcactt caccttgctc atccattaat gtctgagtga aatcttttac cggaccacgc
     4501 agcggatcaa aatcgaattc attagagaaa ctggccatct caggggtaaa tgaaagtgtt
     4561 acctctgtgc ggtcacatcc tgtgaggaat atcgcgagta agcatggtag taatttgtat
     4621 ttcacaacag tcaccagaga gtagagatga ttctcaatca tagtagcaaa tacagtactt
     4681 tacacgttaa atgctatgct taaagaagtt atcttcgcgt aaggagctta tgatgaaact
     4741 ctcaacctgc tgtgccgcgc ttctgctcgc cctcgcctca cccgtggtac tcgccgcgcc
     4801 gggatcctgt gagcgcatac agagcgatat atcacagcgc attatcaata atggcgtacc
     4861 cgaaagcagc ttcacgttaa gtattgtacc caatgaccag gttgatcagc ctgattccca
     4921 ggtcgtcggc cattgcgcta atgatacgca taaaattctc tatacccgca ccaccagcgg
     4981 taacgtctct gctcccgcgc agtctaccca ggatggcgcg cctgccgaac cgcagtaata
     5041 cattatcgac tgaacgccgg atacgacaca tccggcgatt tgaacataca acataatccc
     5101 accttattac tcataccctt ctattgatat gg
//
LOCUS       DAFNBJ010000246         5042 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000246 DAFNBJ010000000
VERSION     DAFNBJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.246"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            43..645
                     /locus_tag="L2X49_004667"
     CDS             43..645
                     /locus_tag="L2X49_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HBN4799635.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIITPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            701..1195
                     /locus_tag="L2X49_004668"
     CDS             701..1195
                     /locus_tag="L2X49_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799636.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIIY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLNLIDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNNDTPKKSTVPNEDK
                     KGIR"
     gene            1195..1461
                     /locus_tag="L2X49_004669"
     CDS             1195..1461
                     /locus_tag="L2X49_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799637.1"
                     /translation="MITGNGINTVTVNGKVKHITELDYITLCLEWTKLREENNRLYEI
                     NNKANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(1653..1934)
                     /locus_tag="L2X49_004670"
     CDS             complement(1653..1934)
                     /locus_tag="L2X49_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799638.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            complement(2040..2315)
                     /locus_tag="L2X49_004671"
     CDS             complement(2040..2315)
                     /locus_tag="L2X49_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBN4799639.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(2315..2599)
                     /locus_tag="L2X49_004672"
     CDS             complement(2315..2599)
                     /locus_tag="L2X49_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBN4799640.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(3503..4534)
                     /locus_tag="L2X49_004673"
     CDS             complement(3503..4534)
                     /locus_tag="L2X49_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBN4799641.1"
                     /translation="MAGLKNTPYNAVHWCQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
BASE COUNT         1271 a         1204 c         1202 g         1365 t
ORIGIN      
        1 tgtgatatta ttaaagagta aaaccagcca gccgaggtat ttatgggaaa aaatcaggaa
       61 gaaaggaaaa ctcccgtcat tgtggtgaaa aaacgtcgta ctttttcgcc accgtcctta
      121 tccgaaaaaa cagatattat cacacctgta tttactgaac agacggcaga atcagccccc
      181 gccggaataa attcatctgc cgtcgaaaca catatccccg aagcgccagc caggaagaag
      241 aaaaagaaaa gacaccgttt tccacgtccc tcccactgga cccgtgagta tactcacgag
      301 tgcgtggaaa aaataaaggc cctgtttcct catcttcgtg ctgaaggtgg tggatttatt
      361 cccctgaaaa ttggcataaa caatgatatt tcggcatttc tggcagaaca cccggagaca
      421 gaactgacca tggacgaatg gctctgtgca gtgtcctgca tcacatcccg ccgcgtatat
      481 ctgcagcgga cagctgtcgc cggcgtccca cgttatggcc tggatggtca ccccaaaggg
      541 caggtctcag actctgaagc acagagtgcc ggtcgcagac tggcgacatt agaacaaaag
      601 tggctcagga tgcaggcaca gcaggagaat atttctgggc aataaacact cctgataaca
      661 ggtggacatt acgtatcaga aaaaatggga agagaatgtt atgtgtgatt cagaagcgga
      721 gaagatttta ttcatcctga ataaacatga acgggcatac agacgcctga tccccgtata
      781 tattttttta gctatagtat tatggatttt acctattctc ctgattatat acctgtattt
      841 ctgtatagtt gacaatgagt ttctcagaat cggtttctgt attgcatgga cagcatacta
      901 ctttctttcg cttttcaagt attttgtaaa atacttcttt cctctcgatc gtgaagattt
      961 gcttaacctg atagatctcg ccaaagattc tcctgatgcg aaacaggagc ttcttgagcg
     1021 tcttttgtcc ggaaaaagaa tgaccgggcg ggatgaaatg gatatcaagc gcattctaaa
     1081 aacaaaggaa gcacttgata acatcaggaa ttttattgat aaacagcagt taacgaataa
     1141 tgatactccg aagaaaagca cggtcccaaa tgaagataaa aaaggcataa gatgatgatc
     1201 acaggaaatg gaataaacac tgtaactgtc aatggaaaag tgaaacacat cacagaactg
     1261 gattatataa cactttgttt agagtggaca aaactacgag aggaaaacaa tcgtctttat
     1321 gaaattaata acaaggcaaa cagaggatgg cgtggtttca tcctaagatt gataggggtt
     1381 aacctgccag ataaaaggac tgagtttaca caacgtctct tgttgacgcg caaaatttcg
     1441 ggttcggtca tgaaaaagta acatattcta ggctggaaca gaccaatttt gcattcgccg
     1501 tccaggagtt tgaggggaaa gtagtcagtg ttttacgctc agtcagggaa agtgtgcaat
     1561 gataaatgac acgaaaggaa tcgtagtaca tgggcaccgc gattcaatat tcaaggaaaa
     1621 aacaatacag caatgtactt gttctgtttc tattattctg gcgatttcaa taaaggcact
     1681 tgctcgcgct ctgtcatttt ctgaaactct tcatgctgca tttcgatttt ccttctaaga
     1741 tcataactcg catacctgtt ctttttatgt atatctatat ctatttttgt gacgagttca
     1801 aaccacaatg ttcgcactcc ccatgcagcg ttatgaaaca tttccgcaac ggctatagag
     1861 tctgtattaa ctgcttcagt catgtagtcg ctaatacgtt cagcggtaat tttcgttatt
     1921 tcatcgtaat tcattttatc tcctacaaga ttatttaatg gaatgcaaaa tatctggcct
     1981 gataataatg ttccagaatt aatatctgca ggctatgtta agttgtcccc acacacaatc
     2041 tacctgtttt ctcgtgtgtg ccacaggcgc aatacataaa tagtctggcc agtaagttcg
     2101 taacggattt cgtactcgcc agcaaaaatc cgtctgacct ccctgggttc aaactgaaat
     2161 aactgttccc ccatacgtgg atgagttaac agaatgaccg gagcctgtgt cagggactgc
     2221 accgttctgg cggccgcagg tttactggcc agcaccagaa aatcatataa ccgcgccaaa
     2281 tcagaaagcg ccttactggt ccacttcagt tccatcagcg tggcaccggt aacggattgt
     2341 cagtactgag gctgtccgcc caggcctgta cagcctgatg gtcgataacc tgtccggatg
     2401 tcacatcgtc cagggcttcc agcgtcaggc gattacgctc ctcctcctgg gcaagccatg
     2461 cagaaagcgc ctgtttgata atccagcccc gggagcgttc cagtctggcg gccatctggt
     2521 cgactttatc agccataggt aatggcacat gcgcggtaat tacttttgtt gcctgtgcgg
     2581 tattgttttt catttgcata tccgattagc tgttaatcat atttaatcat agtgattact
     2641 ttcagacgaa acaaggcaaa taatgcgcgt cagcgcatgt aaggcggcac tccgttgtta
     2701 catatggcac agaaacaaaa ccgccgaagc ccggcgtcag ccggtactga ttgatagatt
     2761 tcaccttacc catccccatc ctgctaagcc atacccgctt tcagccaaga aatagattct
     2821 gtgcgcggtc ggagtggtcc cgacaagggt ttacccgcag tcggggcgta tctccgcgtt
     2881 agcgggccgt cagggccgct tacgagcgtg tactgaggac ttccagccag aagactggca
     2941 gcgatgacaa tgtagttaca acattcataa ttaaaagcga tgttgttccc gcccttcggg
     3001 cgggggcggc ttcgcttttc agttatgagg ggtgggcacg gtgctgcgtt tgtgtggtgt
     3061 tttttattcc tgatgttttc gcggtggttt cagaacggtg aggttcgggg cccgcgcgtt
     3121 agcgtgggag gtgcttttag attctgagcg gcgaggcctt ggccgctgca agcgaaggcc
     3181 tctttgtatt tgttcacagt ggtttcagag ataaagactt tccctgcggc gaagcaacgc
     3241 aggggattaa ataatggggt taggactata ttgcaggttt gctgtttcag gtttgcagct
     3301 ttttaaagtt ctttgtttgc ggatttatgt ggataactga aacaacgtgg ccatgttctg
     3361 atgactgata cgtggtttta tgggcagata tgcccctctg aggggattta aggtgattac
     3421 tgcagatttt gggctgagcg gaatcagcgc cgaaagcccc ggaggggctg gcgatgattc
     3481 agtgctgaag gaatggtgct gtctagtgcg gcggtggcgc agccagcgtc agttcaagct
     3541 gacgcaactc cctgatggcc agtcgcttca gatggtcatc ggaacaaaaa taggcttcat
     3601 ccggcggcag acgcttcctg agatactctg ccatctcatg aatctgctgg tcggcaggca
     3661 gtttcttcag acgtctggcg cgcttactgg ctgccgcttt tgcacgacgg tgtttcagtg
     3721 catcctggct gcgctgcaga taccagcgtt ttctggccgc atgtacggag atatcttcat
     3781 cctcacgcag tacgccttcc cggatgagtt gctggcggat ttcactttca cgcaaccgct
     3841 tctgctgctg ttcgtgaagt ttcaccatgt cgacgcccag catctgccag cctgccggtg
     3901 ttatccagac gtaacgtggc agacgctggc gggtttcacg gtcccacagg gtttcctctg
     3961 aaacacccag cacaccaaaa cgtacctgtt cggccagcaa acgggaaagg cgggagaccg
     4021 tcacttccag ttccggaata accttcccct tgctgtcttt cgggctgatt tctctggcca
     4081 gacgggaaac actcatgcct acggtatgtg tgcccgcatc actgaagctg accagaaccg
     4141 gccagacggc atcgaggagg cgttgttttt ccggacggaa agcatatttg cgaccggcat
     4201 acgttcgttt ctgcacgtaa aaaggatgca gagacatgtg catgcgcagg ctctgctcgc
     4261 cggtcaccgg gtcctttttc accagacggt tgtaggcgtg cccgagctgc ccgctcagcg
     4321 ccttatagcg ctcaggacga taccaggacg gattttcgca tttgggtttg gtggagtgct
     4381 caccacgacg acgcttcgtg cgcttccttt ccggttcaac caggaaagtg gtcgcccttc
     4441 ccgcttcata gtcttcccag aaacggatct gctcttcggg tgctaactga caccagtgta
     4501 ctgcgttata tggagtattc ttcagacctg ccacaagctg tcgcttatgg cggagcgatt
     4561 gtgccgtgta tttatacact gcacagggtt gaatctctgg tacggtttca ttatacttta
     4621 cccgtattct gtgaggcccc cactatcttt cgtcgttccg ccaagttcgt aagaaagata
     4681 gtgggggttt tccttttatg gcttccggaa catcttcagt cttctgactt actgcccgta
     4741 catgccatct ctcctgaatg ccttatggcg cattctgccg tccgactttc gccgctttgc
     4801 tccacagagt gccaccctct gcacagctaa taacagccga aatatacccg ctttacgtta
     4861 atttgaaaag cattacaacg atctcttctc ttccagtgcc tcctcactga tacctctaat
     4921 aagttaatct tgacctgcgt caaaaaaata aaatctattt ttaaaagcag atttttctgc
     4981 tttagatcct tcttttggat taaaagatcg acttttgtcc tgctcacgat catttatctt
     5041 ac
//
LOCUS       DAFNBJ010000247         4998 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000247 DAFNBJ010000000
VERSION     DAFNBJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.247"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..886
                     /locus_tag="L2X49_004674"
     CDS             <1..886
                     /locus_tag="L2X49_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBN4799642.1"
                     /translation="RPVQLAKLAAGDFRIDTCSTTTGLVRYSVLLPYAKQRYVTTDRL
                     ILALPPEIGALVKHYIDEARLLPTDKMFNMGGSAPKFVSQSISQTILSFSPQDYQFAV
                     SCGEAALPSITSTDLRHNVGHSLAMQGASAEEIAHILGHSSLVVAKHYIRATPALALI
                     RAKALGSNPVWQNMVAMMLTGKLVPSKEWEGRRVVGMVGDRLHYEIGGCARTDDECPF
                     CEVRCCYGCLYYRPFLDGHHQGVLNSVSKEVDELIAVSDSVGNALNPLISIHETTQIE
                     IKSVIARCHLHNVRGCGK"
     gene            883..3246
                     /locus_tag="L2X49_004675"
     CDS             883..3246
                     /locus_tag="L2X49_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799643.1"
                     /translation="MKKNIANFETFIIEQKAWFEENLAADFAESWDSFVWICGIKGSG
                     WLRGNGANLLRFDEVNRLKGIDDRHTVSEPYQLFMKAMLVLVYRGRNRSISSAVAVAT
                     LIILKRWYCALIKLTGQTHPIYLTTDVVRSAMDTLSAASRPGDTNLANYKGRCVKIQK
                     LVNHHAFTLVTLQYVSDDCYTNQTNLTRKARETISLKEKDKLDDTSTDGEDTLITIKG
                     FLNIVSLIQRVESGTEKIALNCLLLLIVTGFRSVEAFNLRQDALVKRHIDNSDLSKRL
                     RNKGLPDYFLGIRYVGVKGAGERTHWVEPLAVPLVENIFKSVKLLTAEFRKHIEYLRS
                     KKFSDYLPKPISDITSELVELDDIVKYMVQSSSELRGRAGLRDKASKALEKRGLIPAK
                     VILKSGNEKEKYFTKSDLSHFLKSEFGDNSANTPCTHAWAENGKRYEIKYEELLFLFP
                     KGSLTLKRVLQLKATPLPLNNNGLNKFLGNVTGYVSVFSKYRLLEDDGRPTQLRTHIP
                     RHNINTFLAIAEISDHLQAMLMGRVDITQNQHYQHLALKERRKAASLTPLVPTVPEQS
                     AFTAVDVDSPLDMVKQSGLMTFNSTQTLETNIKANLHTFDDRKDVAGFIEASSGDGLF
                     EDIAAAFEEISKNEGPLQASEMVQRHAVLHPLKLGSCMRDVNLWGCPYRMKCQALKPC
                     EHFTLTGRIDEYSTIAVKGRALNEASLAFEQYIAALPDNQLIQGNIEENLTHLDALSE
                     QLRRRSNLLQVLSAQEILSGEIKVEGEIRTLAQLFALEHHKNKEEEN"
     gene            3246..3689
                     /locus_tag="L2X49_004676"
     CDS             3246..3689
                     /locus_tag="L2X49_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799644.1"
                     /translation="MRGKELDTQIEHELQLMLIEGFDKSPISAKNLHARLKSKGIING
                     GLSTLSNIERRRLIAAYVDQQLSPLNLRPKEKQQYVNRKTRQALLGRNQQLQEENKEL
                     REQLAQNTLSLIEIVKAVKVNTVIPVESLLAPHLIMELHKRNKDQ"
     gene            complement(3887..4219)
                     /locus_tag="L2X49_004677"
     CDS             complement(3887..4219)
                     /locus_tag="L2X49_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4799645.1"
                     /translation="MSEKNPIPERLKEARCRAGLSQRSLGLLIGFDPASASSRMNHYE
                     KGRHVPDIDTLRRMAAELNVPLNYFFCDDQTTAELTLLISRMTEEERSKLIEALKTSS
                     GDNTADKK"
     gene            4260..4820
                     /locus_tag="L2X49_004678"
     CDS             4260..4820
                     /locus_tag="L2X49_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799646.1"
                     /translation="MNSRVTNPESYIFSAIIYIGKDNFTSNEVAKILIERFSLPKNYL
                     KAKAFAYNQIQGLVRKGLLNKVRKLGAYQYLYSATSEFNVVKENVELIEAPKKSPCQF
                     ISSATSHEHSNVNIQIRTLIEKYSSELEKVSGAKEIYEELIITVPSRENEFRKLSLEQ
                     EKKQIKINEKINTLIGIFNKTIAIKQ"
BASE COUNT         1525 a         1027 c         1069 g         1377 t
ORIGIN      
        1 tcgtccggtt cagttagcta aattggctgc tggtgacttt cgaatcgata cttgcagtac
       61 gacaacaggg ttggttcgtt atagcgttct tttaccttat gctaaacaaa ggtacgtcac
      121 tacggatcgg ttgattctgg cgctcccgcc agaaatcggt gcactggtta agcattatat
      181 agatgaagcc agattattac ctaccgataa aatgtttaat atggggggat cagcacctaa
      241 atttgtttct cagtcgatta gccaaacgat tctatccttt agcccacaag attaccagtt
      301 tgccgtatcg tgcggtgaag ccgccctacc ttcaataaca tcaactgatt tgcgccacaa
      361 cgttggtcac tcacttgcaa tgcagggcgc tagtgcagag gaaattgctc acattctcgg
      421 ccattcatct ctggttgtag caaagcacta catccgtgcg accccagcct tggcattgat
      481 ccgcgctaaa gcactcggtt ctaaccctgt gtggcaaaac atggtggcta tgatgcttac
      541 tggaaaacta gttccttcaa aagaatggga aggtcgacgc gtggttggta tggttggtga
      601 tcgattacac tatgagattg gtggctgtgc gagaaccgat gatgaatgcc ccttctgtga
      661 agttcgttgc tgctatggtt gcttatatta ccgccctttc cttgatggcc atcaccaggg
      721 cgtattgaat agcgtctcta aagaagtcga tgagctgata gcagtatcag atagtgttgg
      781 taatgctctc aatccactaa tttctatcca cgagacgacg caaatagaaa tcaagtcagt
      841 gattgcccgt tgtcatttgc ataacgtcag aggttgtggg aaatgaaaaa aaatatagcg
      901 aactttgaga cttttatcat agaacagaaa gcttggttcg aagagaatct ggcagcagat
      961 tttgcagaat catgggatag ctttgtctgg atatgtggga tcaaaggatc cggctggtta
     1021 cgtggcaatg gggctaattt attacgtttt gatgaggtta accggctcaa agggatcgat
     1081 gatcgccata cagtatccga gccttatcag ttgttcatga aagcgatgct ggtactcgtc
     1141 tatcgaggga gaaatcgcag tatttcctct gcggttgctg tcgcaacgct gattattttg
     1201 aaacgatggt actgcgctct cattaagtta actgggcaaa cacatcccat ctatctgact
     1261 acagatgtcg tacgtagcgc aatggatact ctgagtgcag cttcccggcc aggtgatacc
     1321 aacctagcca actacaaggg gcgttgtgtt aagattcaga aattagtaaa ccatcacgcc
     1381 ttcacattag taacattgca atatgtttcc gatgattgtt ataccaatca gactaatttg
     1441 acccggaaag cccgagaaac aatctcacta aaagaaaaag acaagctgga tgatacttcc
     1501 actgatggtg aagatacact gattaccatt aaaggtttcc tcaatatcgt gtcgttaatc
     1561 cagcgagttg aaagcggtac tgaaaagatt gccctcaatt gtcttctgct actgatcgtc
     1621 actggattcc gttctgttga ggcattcaat ctcagacagg atgccttggt caaacgacat
     1681 atagataact ctgacctcag caaacgtctg cggaataaag gattgccaga ttattttctt
     1741 ggcatacgct atgttggtgt taagggggct ggcgaacgaa cgcattgggt cgagccttta
     1801 gctgtacctc tggtcgaaaa tatatttaag tcggtgaagt tactaacagc tgagtttcgc
     1861 aaacatatcg aatatttacg atcaaaaaaa ttttctgatt atttaccaaa gccaatcagt
     1921 gatattacca gtgaactcgt tgagcttgat gatattgtaa aatacatggt ccagtcctca
     1981 tcagagcttc gggggcgtgc aggcttacgt gataaagctt cgaaagcatt agagaaacgt
     2041 gggcttatac cggcaaaagt catccttaaa tcaggtaatg aaaaagaaaa atattttaca
     2101 aagtctgatc ttagtcattt tcttaaaagt gaattcggtg ataacagtgc aaatactccc
     2161 tgtactcatg catgggcaga aaatggcaaa agatatgaga ttaaatatga agagctattg
     2221 ttccttttcc ccaaaggatc actgactctc aagagagtat tgcaactaaa agccacgcca
     2281 ctaccattaa ataataacgg cctaaacaaa tttctaggta acgttactgg ctatgtatct
     2341 gttttcagca aatacaggtt gcttgaagat gacggccgcc ctactcagct ccgaacgcat
     2401 atccctcgac acaacattaa tacttttctc gcaattgcag agatctcaga ccacttacaa
     2461 gcgatgttga tggggcgtgt tgatattact caaaatcagc attatcagca cctggctctc
     2521 aaggaacggc gaaaagcagc ttctctaacc cctttagttc caaccgtacc ggagcagtca
     2581 gcatttactg ctgttgacgt tgattcacct ctcgatatgg taaagcaaag cggtttaatg
     2641 acgtttaata gtacccaaac tctggaaacc aacataaagg caaacctgca tacctttgat
     2701 gatcgtaaag atgttgctgg tttcattgaa gcgtcgtcag gggatggatt attcgaagac
     2761 atagccgctg cgttcgagga aatcagtaaa aacgaagggc cattacaagc gtctgaaatg
     2821 gtacaaaggc atgcggtatt gcatccatta aaactgggct cctgcatgag agacgttaac
     2881 ttgtggggat gcccttatcg aatgaagtgc caagcattga aaccctgtga gcatttcacg
     2941 ttgacaggtc gtattgatga atactcaact attgcagtaa aaggtcgggc attaaacgag
     3001 gcttcgctcg cttttgaaca gtatatcgcg gcccttccag acaaccagtt aatacaaggc
     3061 aatatcgaag aaaatctaac tcacctggat gccttaagcg agcaattacg caggcgctca
     3121 aacttgttac aagttctctc tgctcaagag attctgtctg gagagataaa agtggaaggt
     3181 gaaattcgta ctttagctca attatttgcc cttgagcacc ataaaaacaa agaggaagaa
     3241 aactaatgcg tggtaaagaa ttggatactc agatagagca tgaactccag ttaatgttga
     3301 ttgaagggtt tgataaatcg cctatttcag ctaaaaactt acatgccaga cttaaatcaa
     3361 aaggaatcat taatggtggc ttaagtacat taagtaacat tgaacgaagg cgtcttattg
     3421 cagcctatgt cgatcaacaa ctatcgcctc tgaatcttcg tcccaaagaa aaacagcagt
     3481 atgtgaaccg taagactcgg caggccttgc ttggtcgtaa tcagcagtta caggaagaga
     3541 ataaagagct tcgcgaacaa ctagctcaga ataccttgtc attgattgaa attgtcaaag
     3601 cggtaaaagt caatacggtt ataccggtag aaagccttct tgctccgcat ttgatcatgg
     3661 aattacacaa aaggaataaa gatcaatgat agatatagcg gtatattcgc actacgaact
     3721 catgaataaa catcttgctc tattcgaaag attagcattg caactttcaa cgcccactca
     3781 ttagagtcca ttttatcaga gggcgtctac tactgtaatg ccctgcttgg tttattgcac
     3841 tgcacttttg ttaccttcaa aaagtttagc aggcgtttgt acaacatcat tttttgtcag
     3901 cagtgttgtc acctgaagac gttttgagtg cttcgataag tttacttcgc tcttcctctg
     3961 tcattcggga tatgagtaat gtaagttctg ctgtcgtctg atcatcacaa aaaaagtagt
     4021 tcaacggcac gtttaactct gccgccatcc gtcgaagcgt atcaatgtcg gggacatggc
     4081 gccctttctc atagtgattc atacgactac tagccgatgc gggatcaaag ccaataagta
     4141 gccccagaga gcgctgtgat agtcccgccc tgcatctcgc ttctttaagt cgttctggta
     4201 tcgggttttt ttctgacact gaatcatctt catcggcctc gacagccttt tttagtgata
     4261 tgaatagtag agtaaccaac cctgagtcgt acatattttc tgcaatcatc tatattggca
     4321 aagataactt caccagtaac gaagtagcga aaattcttat cgagcgattt tcacttccga
     4381 aaaattattt aaaggcaaaa gcttttgctt ataaccaaat tcaaggtctg gttagaaagg
     4441 gattgttaaa taaagtaaga aaattaggtg cgtatcaata tttatactca gctacatctg
     4501 aattcaatgt cgtaaaggaa aacgtagagt taattgaggc acccaaaaaa agcccctgtc
     4561 aatttatttc aagtgctacc agtcatgaac atagcaatgt aaacattcaa ataagaactc
     4621 ttattgaaaa atacagcagc gagttggaaa aagtatcagg agcaaaggag atttatgaag
     4681 aattgataat taccgtgccg agcagggaaa atgaattcag gaaactttct ctcgaacaag
     4741 agaaaaagca aattaagata aatgaaaaaa taaacacatt gattgggatt tttaataaaa
     4801 ccattgcaat aaagcaatga cttttaaaaa acatttaaag ttaaataagt tcatgaaaga
     4861 gagagggcga agcagagtcc gccctctccc ttcataccct gatcagcctc attacaatga
     4921 ggttaacagc gtacaccact atatgctagt ccttaataaa tataatccca gccagagcga
     4981 gatgcaagtt ttgagcgt
//
LOCUS       DAFNBJ010000248         4923 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000248 DAFNBJ010000000
VERSION     DAFNBJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.248"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            218..391
                     /locus_tag="L2X49_004679"
     CDS             218..391
                     /locus_tag="L2X49_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004136225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBN4799647.1"
                     /translation="MSDVLYSGRKSQSFQLRLPARMKEEIRRVAEMDGISINSAIVQR
                     LAKSLREERANAQ"
     gene            381..1091
                     /locus_tag="L2X49_004680"
     CDS             381..1091
                     /locus_tag="L2X49_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799648.1"
                     /translation="MPSKNSEAQQCANTNWASMSVTVCKETNMNIVAKSDYNFHGVEL
                     VPTRDMHGVWFTSSNIASALKYANSRAVTMIYNKYSDEFSAGMTQVLEVSTSGNYRKK
                     VRVFSLRGAHLIAMFARTPVAKEFRRWVLDILDRQAECSPIAKQFTDEELVNLCYLQL
                     WMEKSQQMCKHIYPGMKQIGSELSGRIYDIAYETRYMSEETKKSLLREMKNLDTNNFV
                     VKNAQPMLAKLRGEEWIH"
     gene            1179..1907
                     /locus_tag="L2X49_004681"
     CDS             1179..1907
                     /locus_tag="L2X49_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799649.1"
                     /translation="MTFKNVANIGSVVTDKTIDSQRLLEMVNQARRQCGEPEVRNNKF
                     IEKIEDELDGEHYTKSVVQKSNKTSMVIIEMSIKQALRVAARESKAVRRSLVDQLESM
                     QEAHIKSGKSASGLVEYRQARTLKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVG
                     FNAIPLPAIEEHYYSAGEVAEQLGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQ
                     VEAFRYNAEGVKALQHLIHGSNVA"
     gene            1907..2128
                     /locus_tag="L2X49_004682"
     CDS             1907..2128
                     /locus_tag="L2X49_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799650.1"
                     /translation="MAKKKYGIMPPRIKGRARVKGDAGRYHILGVLWHERALILSRPH
                     GYIEKVSIDRVEILPLTPEEEETYGLFDN"
     gene            complement(2143..2430)
                     /locus_tag="L2X49_004683"
     CDS             complement(2143..2430)
                     /locus_tag="L2X49_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBN4799651.1"
                     /translation="MPFHPFGEALMMYSKYDEAQFHLRLTHELHAKIKQRAKMNNRSI
                     NSEIVATMEESLSKPSPVSGYRDEEERLASLISERVKEVAADILRKEKTRD"
     gene            2533..2871
                     /locus_tag="L2X49_004684"
                     /pseudo
     CDS             2533..2871
                     /locus_tag="L2X49_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT         1563 a          925 c         1112 g         1323 t
ORIGIN      
        1 ttcgtttagc cctttccgca atagcactac gcatgccgtc aggcattcta accgtaaatc
       61 tttctatgaa atttgattcg tctttttctg acattttctt attcgcaagg catatgagca
      121 ataaaaaaca cagtagcatc atattgacat tatgagcaat ggcatcataa tggtgtcatg
      181 acatcatggt gatgtcatta tgacggagat attgatgatg agcgatgttc tttacagtgg
      241 tcgtaaaagt cagagtttcc agcttcgttt gccagcgcga atgaaggagg agattagacg
      301 tgttgctgaa atggatggaa tttctatcaa ctctgcgatt gtgcagcgac tggctaaaag
      361 cctgagagag gaaagagcta atgcccagta aaaatagtga agcccagcag tgcgcgaaca
      421 caaactgggc ctctatgtca gtaaccgtat gcaaggaaac taacatgaat attgtagcaa
      481 aatcagatta caacttccac ggagttgagt tggtgcccac ccgtgatatg catggtgttt
      541 ggtttacatc atctaatatt gcatctgcac ttaaatacgc aaatagtcgt gcagtaacaa
      601 tgatttataa caagtatagc gatgagttta gcgccggaat gactcaggta ctcgaagtga
      661 gtacctcagg aaattatcgc aaaaaagtgc gagttttctc actacgcggt gcccacctaa
      721 tcgcgatgtt tgctcgcact ccggtagcca aagagttccg ccgctgggtg ctggatattt
      781 tggatcggca ggcagaatgc tcaccgattg caaaacagtt tactgacgaa gaactggtta
      841 atctctgcta cttacaattg tggatggaga agagtcaaca aatgtgcaaa cacatctacc
      901 caggaatgaa gcaaattggt tctgagcttt caggaaggat ttacgatatt gcatatgaga
      961 ctcgctacat gtcagaagaa accaagaaat cacttcttcg tgaaatgaag aatcttgata
     1021 ccaacaattt tgtcgtaaag aacgctcagc caatgctggc aaaactgcgc ggcgaggaat
     1081 ggattcattg attggtgcac aggacggcgc aaaagaaaac cgccagtgtg ctgctggcgg
     1141 cctatgtcac acccttacta ccacataagg aatgcctaat gacttttaag aatgtagcaa
     1201 acatcggatc cgttgtcacg gataaaacca ttgacagcca gcgcctgctt gagatggtaa
     1261 atcaggctcg tagacagtgc ggtgagccgg aggttcgtaa taacaaattc atcgagaaaa
     1321 ttgaagatga attggatgga gagcactaca caaaaagtgt agtgcaaaaa tcgaacaaaa
     1381 cttcgatggt tatcattgag atgtcaatca agcaagcatt gcgagttgcc gctcgcgagt
     1441 caaaagccgt tcgccgctca ctggtagacc aacttgaaag tatgcaagaa gcgcacatta
     1501 aaagcggtaa atcagcgagt ggacttgttg agtatcgtca agcgcgaaca ttgaaaatga
     1561 cggttgaagc tgttaccaat ctgttcgatc tgatgccaaa tcttgcgcct gaggcaaagc
     1621 agacagcggc agcaagtata atcaacccgc tcgttggttt taatgcaata cctcttccgg
     1681 caatagaaga gcattactac tcagcagggg aggttgcaga gcagcttgga gtaacggcca
     1741 acaagattgg tcgcattgct aacgcaaaca acctcaaaac tgagcagtac gggaagttct
     1801 tcctggataa atctgcgcat tccagcaaac aggtggaagc attccgctac aatgcggaag
     1861 gtgttaaagc actacaacac ctgattcatg ggagtaatgt tgcataatgg caaagaaaaa
     1921 atatggcatt atgccgccaa gaatcaaagg aagagctagg gtaaaaggtg atgctggaag
     1981 gtatcacatt cttggagttc tgtggcatga gagagcttta attttaagta gacctcatgg
     2041 gtacattgaa aaggtatcta tagatagagt agagattctt ccccttacac ctgaagaaga
     2101 agaaacgtac ggactttttg ataactaacc aactaaaccc gcttaatcgc gggttttttc
     2161 ttttctaagg atatcagccg caacttcttt tactcgttcc gagattaatg aggccagcct
     2221 ctcttcttca tcacgatacc cgcttacagg tgatggtttg gagagtgatt cttccatcgt
     2281 agccacaatt tcggaattga tagacctgtt attcattttt gcacgctgtt taatcttggc
     2341 gtgcaactcg tgcgtaagcc tcaagtggaa ctgcgcctca tcgtatttgc tgtacatcat
     2401 caatgcctca ccaaatgggt ggaatggcat cgtaaaacct actgtacaaa tcaacaatcg
     2461 taccgtttcg gtatgcaaca aataccaacc gtagccacgc tgcggtgatt ccttgtatct
     2521 ggagcaaatt aaatgacaga cattacagcc aatgttgtag tgagtatgcc ttcgcaactc
     2581 ttcactatgg cgcgttcttt taaagccgta gccaatggca aaatttatat cggtaaaatt
     2641 gacacggacc cggtaaatcc tgaaaaccaa attcaggttt atgtggagaa cgaagacggc
     2701 tctcacattc ccgtttcgca accaataatt attaacgctg ctggctaccc tgtatataac
     2761 ggacagattg ctaagttcgt tactgtacaa gggcattcta tggctgtgta cgatgcatat
     2821 ggatcacagc agttctattt ccctaatgtg ctgaagtacg atccagatca gttttcatat
     2881 ttgctgaaaa gtgatgatgg ctataagtat ataggagaat gcaaagatat atctacattg
     2941 agaacaattg aaccaagtta tgatggacag gttataagac tgaggggata ttactctgac
     3001 agctattcag gaggtggaag gtttttccgt agtgttaatc ctgatggatt gacagataac
     3061 ggaggaacga taattttcac atcagaagga aatgtatgga aatcagatga tatatataat
     3121 gttatgcttg aggatttcgg agctaaagat ggtgctgaca acaccgaagc atttcataaa
     3181 gcaattaact caccagcaaa aaaaatcaca acaaatataa aagagttgat actaacaaac
     3241 aatattgtaa ttacacgcgg cgacctggca attgatatgc ctgatacgga gataatatgg
     3301 aatgctccgg aaggacatat tcctgataga agtggatcta caaatccaag taactatcgt
     3361 tttcctggca ttctagcatt tcgcggcact gctggagata taatagactc ttttactcta
     3421 acaaataaaa tatcaaaagg agatacaaca tattggtgta cgaacaactc tctttttgag
     3481 aatagacagt gggttattat gtcatcggat gttggcgggg gaaccgtagg tagagaaata
     3541 aatgtaatga cacaagtgca gggcagtggt ggagcaataa ctcaattgcg agttgattat
     3601 aaaacaggct ggcatcttga tgtcggaagg gtattgacat atagaaatgt aaccccaata
     3661 gaaaatgtaa gaattgcttt taaaggcgta aaatggaatc aaaacataac agatgcttca
     3721 ggtggtggcg atgaatttgc cagtcaacaa tcatgcgcac ttgtgtccct tgaatacgta
     3781 aataacgctg acatttgtct cgggtatgga tttaatcacc cttacccaat ggttgtaact
     3841 gccatggtca ggaatgtttt tgttcatgat acggaaacaa atttcccaag agttccaggg
     3901 tccgatcatg ttgttcaatt caataacgca tatgaatgcc acgcaagaag attacgcaat
     3961 atttcaggca ggcacgtagt tgattttagt ggtgcaagtt attgctctgt aagagattgc
     4021 ggagaaacag gaacaagaaa tggtgcattt acaacgcacg gtatgtttga gcacaatttg
     4081 cgatttgata ataattatgg tcttttaagc attgcaaaca gtggtgaata ttatggtgag
     4141 tcagcagata atataactgt aacccatcat ttcggagatt atttaattgc aacttcaaaa
     4201 gttaccaacc ttaacatatt gcatgcaata tttacaaaag gtgcaagatt aaacaatgat
     4261 gccgtcacat tgattgatgt tactattgga gaaaactcaa ctaatgccga tagagggctt
     4321 agatttaccc aaagctctaa tgtttatggg cgtggggcaa aaatattcgg ctgtgacatt
     4381 gtgttaaaca agcaagcaga gaatgctctg cctgattctc ttaaccaaag ggtgaaaatt
     4441 gaatcgacat atatcaggaa ttcaaacggt tcatattatg gtggtagttg gattgaacta
     4501 attaattgtg atgtttcagg atcaggttca agtcttgaga atgttgttgc tgcaacgcgg
     4561 tttagcatgc tgtccggatc attatctaat actggtttta tttttcaagg cccatcagag
     4621 caaatcgtaa aaatacatga cgtttcacag tctggaacag gagcgtcagg gcttaatagt
     4681 cattttactt taaataaaga tgaggcagga agtggaccaa taacaatgtc atataaaaat
     4741 aatcaatgta tattgagtag agaaacatgc gcattcaaaa taaataatgc ttcaggagca
     4801 ttcagagtaa actctacagg gaatacgttt caaggtggga aaatagagtt tcagaaatct
     4861 attacatcag gtggcagtta cttaaatcac actggaaacg tagagcgtgg cgttagtaga
     4921 gtc
//
LOCUS       DAFNBJ010000249         4908 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.249, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000249 DAFNBJ010000000
VERSION     DAFNBJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.249"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(44..805)
                     /gene="kduD"
                     /locus_tag="L2X49_004685"
     CDS             complement(44..805)
                     /gene="kduD"
                     /locus_tag="L2X49_004685"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBN4799652.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(835..1671)
                     /gene="kduI"
                     /locus_tag="L2X49_004686"
     CDS             complement(835..1671)
                     /gene="kduI"
                     /locus_tag="L2X49_004686"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBN4799653.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1958..3139)
                     /locus_tag="L2X49_004687"
     CDS             complement(1958..3139)
                     /locus_tag="L2X49_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBN4799654.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3394..4623
                     /locus_tag="L2X49_004688"
     CDS             3394..4623
                     /locus_tag="L2X49_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBN4799655.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1282 a         1250 c         1074 g         1302 t
ORIGIN      
        1 aaaccctgcc atgcggcagg gtcataaaag taagaagaat gaattaacgc gccagccaac
       61 cgccatccac ggcaatggta taaccattca cataatctga agcgctggag gcaaggaaca
      121 ctaccggccc catcaggtca ctcggcagtc cccaacggcc agctggaatg cggtcgagaa
      181 tttccgcgct acgttgttca tctgcccgca gttgttgagt attgttggtc gccatgtaac
      241 ccggagctat cgcattaaca ttaatgttgt gtttagccca ttcgttcgcc atcaatcgcg
      301 tcacgcccat cacaccgctt tttgatgcgg tataagaagg cacacggatc ccgccctgga
      361 aggagagcat tgacgcgata ttgataatct tgccgccatt gccttgcgcg ataaagtgtt
      421 tcgccgctgc ctgagacatg aagaatacgc tcttgatatt caggttcatg acatcgtccc
      481 agtccttttc gctgaactcg agagcgtctt cgcggcgaat caatccggcg ttattcacca
      541 ggatatcaat atgaccaaac tccgctaccg cgcgatccag cagtgctgga ataccatcaa
      601 tctttcgcag atcggcggtc aggcttaaaa aacgacgccc cagcgctgtg acctgcttga
      661 tggtttcagt cggttcaacg atgttaatgc caacaatgtc acagcccgct tgcgccagcc
      721 ccaacgccat cccctggccc agtccagtat cacaaccagt gacgaccgca actttacctt
      781 cgagagaaaa tgcacttaaa atcataacaa taccttattc gtttatgccg acaactagcg
      841 caaatcttta acggccacat ggtccatatc atcaaagacc tggttttcac cgaccatgcc
      901 ccagataaag gtataagctt tggttccgac gccggaatga atcgaccagc tcggggagat
      961 caccgcctgc tcgttatgca tcacaatgtg acgcgtttct tgcggctgcc ccatcatgtg
     1021 gaaaacgcag gcgtcatcat ccatattgaa atagaaataa acttccatcc ggcgctcgtg
     1081 ggtgtgacac ggcatggtgt tccacaagtt acccggagcc agctccgtca gccccatact
     1141 caattggcag gtttccagca catccgggac aaaatatttg ttaatagtgc gacggttact
     1201 ggtgaggtta tcgcctaacg tgactggaga tacttcgtcc ggggtgactt ttttggtggg
     1261 atacgtcgta tgcgcgggtg cgcaattgta ataaaacttt gccggagtgg cggtatcaat
     1321 actggcaaag acaacttctt ttgcaccttt acccacatac agggcgtcgc ggtgaccgat
     1381 ttcatagcat tggccatcga cagtaatcgt accggcaccg ccaatattga taacacctaa
     1441 ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta ccaacttccc cgccaacgga
     1501 aaccgtttta gttatcggca taatgccgcc aacaataatt cggtcaatgt ggctgtaaac
     1561 catggtgtac tcatcggcga caaatacctc ttcaaccaaa aattcattgc gcagcccttg
     1621 ggtatccagc gtttttgcgt gcgcactgtg gatgctctgt cttacgtcca catcaacctc
     1681 cgataatcag tgaagcgtga aaatagaaaa cgatagtgaa acactgtttc gttttctcta
     1741 taaagggatg atatgcccat gggctagggt tttcaattaa aaataaaaca atgtttcatt
     1801 tttaagttaa ggattaaaaa aagtgcagtg tcgatcacga atatgtcatg agaatggaaa
     1861 aatgagggag cccagaaaat gtatgtcgac ttgccagcca tcattattca acagattaat
     1921 gacggcagat atttatcggt aaggaggaaa tgcagagcta ctcgtcacgt tcaatggtca
     1981 atgccacgcc ctggcccccg ccgatacaaa gcgttgccag ccctttgcgg gcattacgtt
     2041 tcaccatttc atgaaccaga gaaaccagga ttcggcaacc ggaagcgcct atcgggtgac
     2101 cgagtgcgat cgcgccacca ttgacattga cccgacgctc atcccactca agcatcttgc
     2161 caaccgaaag cgcctgtgca gcaaacgctt cattagcctc gataagatcg acatcagcca
     2221 actgccagcc tacacgctcc aggcaacggc gggtcgcata caccggcgca attcccatca
     2281 atgccggatc tacaccaacg ctggcaaatg cgcggatgcg ggccagcacg ggtaaattca
     2341 acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg tttatggatg
     2401 atgcattccc cgcggtcaca gaaccgagac tatcaaatga aggatttaaa cgggctaaac
     2461 cttctgcact ggcgtcagtg cgtggctgtt catcggtatc aacaaccaac gtctgaccgt
     2521 tactttgggt cattaccggg acgatctcat ctttaaatcg tccggcgtca atcgccgctc
     2581 gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa ttgacggctg atgccatatt
     2641 cgcgagccag attttcggcg gtgacaccaa tatgataatc attgaaggca tcccataacc
     2701 catcatgcac aagactgtca accagctggc tattgccaag ctgtgcaccg gtgcggctat
     2761 cagtcagaac atgtggtgcg cggctcatgt tttcctggcc accggcgatg acaatatcag
     2821 cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc tttaagtccg gaaccgcaga
     2881 cgtcattaat cgtgattgca gaaacgctat taggcagacc acctttaata gccgattgcc
     2941 ttgccggatt ctgccctgcc cctgcagtca acacctgacc aagaattact tcatccaccg
     3001 catatgcagg aacgccggta cgttctatta acgctttcac gaccagacta ccaagttcca
     3061 cggcggaatg acccgctaac gcaccgcgaa agcagccgat aggtgtccgt aacgccccga
     3121 caatcacaac gtctttcatc acaacctcgc accaattaac agtgcaatag taaatgattg
     3181 ttaattacag ttatcttaat tgtttaaaaa aagtgatttt tatcacaaag gaaatatgcc
     3241 tgagcagtag tcagagtcat aactggcacg taaggtttgc aaccactaac ccaccaatag
     3301 aggggtagat agggcgttaa tctcccatac ttaacctggt ttatggtaaa ttgccctcca
     3361 ttttgtttaa tttgtagatg atacgttcag ataatgtcta atatttggtc taaagaagaa
     3421 actctgtgga gtttcgcgct ctacggcaca gccgttggtg caggcacgct cttccttcct
     3481 attcagttag gttcggcagg ggctgtggtc ctgtttatta ctgctctggt cgcctggcct
     3541 ttaacatatt ggccacataa agccttatgc cagttcatcc tctcatcgaa aacatcagca
     3601 ggtgaaggga taacgggcgc agtaacacac tactatggca agaagattgg taatctgatt
     3661 accacgctgt acttcatcgc cttttttgtc gtcgtgttga tatatgcagt ggcaattacc
     3721 aactcactta cggaacagct ggcaaagcat atggttattg atcttcgcat ccgtatgttg
     3781 gtgagtctgg gtgttgtatt aatcctgaat ctcatttttc tgatgggacg ccatgcaacc
     3841 attcgggtaa tgggtttttt ggtattccca ttgattgcct atttcttatt tctttccatt
     3901 taccttgtcg gtagttggca acctgatcta ttaacgaccc aggtagagtt caatcagaat
     3961 acccttcacc agatatggat atcgattccc gtgatggttt tcgcctttag ccatacgccc
     4021 attatttcta cgtttgccat agacagacgt gaaaaatatg gcgaacacgc tatggataaa
     4081 tgcaaaaaaa ttatgaaagt cgcttatctc atcatctgca taagtgtact gttctttgtc
     4141 tttagctgcc tgctttctat tccaccttcg tatattgaag cggctaaaga agaaggggtc
     4201 accattttat cggcgctttc tatgctgccg aacgccccag catggctgtc aatttccggg
     4261 attattgtcg cagtagttgc gatgtcgaaa tcattcctgg gtacgtactt tggcgttatt
     4321 gaaggtgcca cggaggtcgt caaaacaaca ctacagcagg ttggtgtaaa gaaaagtcgt
     4381 gcatttaacc gcgcactatc aattatgttg gtatcgctga tcaccttcat tgtttgttgc
     4441 attaacccga acgcgatttc gatgatttac gcgatcagcg gcccgctcat tgccatgata
     4501 cttttcatca tgcctacgtt gtcaacgtat ctgatcccgg cgcttaaacc ctggcgttcc
     4561 atcggaaatc tgattacgct gatcgtgggt atcctgtgcg tatcggtaat gttctttagc
     4621 tgatatccat tgtgaatggt tcaaaattgc tacaattttg aaccattcca gttttcataa
     4681 tgttcattcg ttattattcc gcactatcac ttccctcatc atatcctagt attatcagtt
     4741 agttaaacac acaaagagat ctgaacatct taaataagac tatttaagat gcataactta
     4801 gattcgcaag atatcttctg gtcattcaaa aacaatttgc gatatagata ttctcatacc
     4861 atgagatagt tatctgaacc accctcaacc aggtaagatt aaatcagt
//
LOCUS       DAFNBJ010000250         4905 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.250, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000250 DAFNBJ010000000
VERSION     DAFNBJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.250"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            69..1043
                     /gene="hemB"
                     /locus_tag="L2X49_004689"
     CDS             69..1043
                     /gene="hemB"
                     /locus_tag="L2X49_004689"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBN4799656.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1149..2000)
                     /gene="tauD"
                     /locus_tag="L2X49_004690"
     CDS             complement(1149..2000)
                     /gene="tauD"
                     /locus_tag="L2X49_004690"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBN4799657.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(1997..2824)
                     /gene="tauC"
                     /locus_tag="L2X49_004691"
     CDS             complement(1997..2824)
                     /gene="tauC"
                     /locus_tag="L2X49_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBN4799658.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2821..3588)
                     /gene="tauB"
                     /locus_tag="L2X49_004692"
     CDS             complement(2821..3588)
                     /gene="tauB"
                     /locus_tag="L2X49_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBN4799659.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSILLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLSLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3601..4563)
                     /gene="tauA"
                     /locus_tag="L2X49_004693"
     CDS             complement(3601..4563)
                     /gene="tauA"
                     /locus_tag="L2X49_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN4799660.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1147 a         1405 c         1242 g         1111 t
ORIGIN      
        1 ttcgtaaaac actcctgccc cgcttcaggt atactgtgca ccttaattcc acaaacatca
       61 ggcagaccat gacagactta atccaacgcc ctcgtcgcct gcgcaaatct cctgcgctgc
      121 gcgctatgtt tgaagagaca acacttagcc ttaacgacct ggtgttgccg atctttgttg
      181 aagaagaaat tgacgactac aaagccgttg aagccatgcc aggcgtgatg cgcattccag
      241 agaaacatct ggcacgcgaa attgaacgca tcgccaacgc cggtatccgt tccgtaatga
      301 cttttggcat ctctcaccat accgatgaaa ccggcagcga cgcctggcgg gaagatggac
      361 tggtggcgcg tatgtcgcgc atctgcaagc agactgtgcc agaaatgatc gtcatgtcag
      421 acacttgttt ctgtgaatac acttctcacg gtcactgcgg tgtgctgtgc gagcatggcg
      481 tcgacaacga cgcgactctg gaaaatttag gcaagcaagc cgtggttgca gctgctgcag
      541 gcgcagactt catcgcccct tccgccgcga tggacggcca ggtacaggcg attcgtcagg
      601 cgctggacgc tgcaggcttt aaagatacgg cgattatgtc gtattcgacc aagttcgcct
      661 cttcctttta tggcccgttc cgtgaagctg ccggaagcgc attaaaaggc gaccgcaaaa
      721 gctatcagat gaacccaatg aaccgtcgcg aggcgattcg tgagtcactg ctggatgaag
      781 cccagggcgc agactgcctg atggttaaac ctgctggagc gtacctcgac atcgtgcgtg
      841 agctgcgtga acgtactgaa ttgccgattg gcgcgtatca ggtgagcggt gagtacgcga
      901 tgattaagtt cgccgcgctg gcgggtgcta tagatgaaga gaaagtcgtg ctcgaaagct
      961 taggttcaat taagcgtgcg ggtgcggatc tgattttcag ctactttgcg ctggatttgg
     1021 ctgagaagaa gattctgcgt taattttttt cctctcagaa agggggagtt aacctgttga
     1081 aagtacggtg aacgccccct caccctaacc ctctccccaa gggggcgagg gaaccgtccg
     1141 cactcgtatc accccgcccg ataaaacggt ttatccccaa gaatcgtcgc ccgatgcata
     1201 atccgccgct gtggcaggta atcggcattg gcatagtgct gagtcacgcg gttatcccaa
     1261 atcgcaatat cattcggttg ccagcgccaa cgcacctgaa actccggttt agtgatatgg
     1321 gcgaataaaa agcctaacaa ggcttcgctc tctttctcgc tgacatcgac aattcgcgta
     1381 gtaaagcctt cattcacaaa cagcgcctgt ttaccgctca ccggatgcgt tcgcaccacc
     1441 ggatgtagca acggaggatt tttcgcgacc gcctcgcgcc agcgttgatg ttcctcctcg
     1501 gttttgcggt atttgtattc cgggaacgat ttacggaaat catgctccgc acgcagcccg
     1561 ctcagcagct ggcggaaggg aacagagagc gcctcatagg ccgcaatacc gctggtccag
     1621 agcgtatcac cgccggtcga aggtaattct ttagctgcca gaatcgcccc tgcgggtggc
     1681 gtttcaataa atgtcacatc ggtatgccag ttgtcgttat ctggcggatt atcgttatgg
     1741 gtatccagca cgatgatctc gtcaacccct tcggcatgag ggtaaacagg gtgaatatgc
     1801 agttcgccaa aacgctgggc cagcgcgcgt tgctgctgcg gcgtaatggc ttgatcgcgc
     1861 agaaacacca cctgatggcg cagcaccgca tggtaaagct gttcaaactg attatcgctt
     1921 aacgggcgcg tcaggtcggc acccgaaatt tgtgcgccga tatacggccc cagcggggta
     1981 atgctcagac gttcactcat tgtacttctc catgccaggg cgtcagacgg cgctgtaacg
     2041 cgcgcaaacc cagttctaaa agaaaggcga taatcgcaat caccgcgatt cccgccagca
     2101 ccacgtcagt cgcgagaaat tcacccgctg actgaaccat aaatcctaaa ccgcgcgtcg
     2161 cggcaatcag ctccgccgct accagcgtag accagcccac acccagacca atgcgtaatc
     2221 cggtgaggat ttccggcagc gcgccgggca aaatgacaaa ccacagcacc tgcgcacggc
     2281 tggcacccag cgactgggcg gcacgaatgc gaacctgctg cgcgcttttc acccccgcca
     2341 gcgccgacat cgccaccggt gcaaaaatcg ctaaatagat cagtaagatc ttcgaggttt
     2401 caccaatacc aaaccagatc accatcagcg gcaaataagc cagcggcggc accggacgat
     2461 aaagttcgat taccggatcc agaatgccgc gcaccgtcgg gcttagcccc atggcgatcc
     2521 cgaccggaat accgatcaac accgccgcga gtagcgccag cacaatgcgc gtcaggctgg
     2581 ctgccagatg ctgccacagc gtggcgtcca taaatccctg cggcccggca atggtgagca
     2641 gttttgccag tacctgttgc ggtggcggca aaaacagcgg gctaatcagt tgcagcgctg
     2701 cgactgccca ccatacagta agtaaaaccg ctaacgtgcc aatgcttaag gtcacctgac
     2761 gcgagagcgg ccagcgccat ttaagccgat gcgaatgcag tttttcatta atgagcacac
     2821 tcatgagaac gcctcccgct gctcaaatac gcggcttaaa acatattcgc gcatggcgat
     2881 gaattgtgga tcggacttga tgctgcggct cgactctccc gcaacaaagc ggcgagcaaa
     2941 gttgagcgac agccgctcca gcacacggcc agggccggat gaaagcagaa ccagttcagt
     3001 cgccataaac accgcttctt ctatatcgtg ggtaatcaac agcacctgct tgcccgtctc
     3061 ctgccagagt ttcagcagca gggtttgcat ctggtcgcgg gtgaaggcgt ccagcgcacc
     3121 aaacggttcg tcgagtaata acagctgggg attcgccgcc agcgcacggg caatccccac
     3181 ccgctgacgt tgaccaccgg aaagctgcca gatgtagcgt ttttctgcgc cttccagccc
     3241 cacttttttc agcatctggt gcgcgatttc cagtcgctgc attttctcta tacctgccaa
     3301 ttgcaggccg aacgccacgt tgtcctgtac attgcgccac ggtagtagcc cttcattctg
     3361 aaaaactacg ccacgctctg ctcccggtcc ctcaatacgc ttacccgcca gtagaatgct
     3421 gccatgctga taaggcacaa aaccggcaat cagattcagc aacgtggttt taccgcagcc
     3481 ggacggcccc agcaccacca gtagctcgcc gctttccagc gtcaggttga tatcttccag
     3541 tgccggtttg ccgccataat cggcgtaaag atgagagatt tgcagcatct gcgcctcctt
     3601 ttattgcacg aagcgcgagg taacgtactg gctgtaatca ttcgctacag ccgggacctt
     3661 gccctgctct ttcaaaaact gcgcggtgtc gatgatcgct ttgttcaccg gtccggtcag
     3721 ttctgccgtt tgttgctgcg gcgtcagata ggtattcccc ttcaccagcc ccggaacgtc
     3781 accttcaggc acgccgctta aacgcgccag tttgctgatg ttttccggct gtttcagcca
     3841 cacgtctggg ttagcaatgt acggttgctg agcatcgatg gcgcttttag cgaacgcttt
     3901 cacgacctca ggatgtttct cggcaaaatc tttgcgcacc acccagacgt ccagcgttgg
     3961 cgcgccccac tgcccgacct gttcagaatc ggtcaacacc ttgccgtctt tttccagggc
     4021 gttaaccgcc ggtgcccaga cataagcacc atcaatatct ccccgctgcc aggcagcgat
     4081 aatcgcgggc ggctgcaggt tcacaatctc cacttgcccg ggtttaatgc cccagtgttt
     4141 cagcgccgcc agcaggctgt agtgggtggt ggagataaac ggtacggcga tgcgtttgcc
     4201 aatcagatct tccggtttgc tgatagtttt ctttaccacc agcgcttcgg agttacccag
     4261 ttttgacgcc agcaagaaga cttcaatcgg cacctgttgg ctggctgcaa ccgctaacgg
     4321 gctggaaccg aggttgccga tttgcacatc gcctgaagcc agcgcccgca cgatgctggc
     4381 tccgctgtca aacttacgcc agtccacgct cgccccgctc tctttagcaa aggtgttgtc
     4441 ggcctgagcc actttcgccg gttcggctga ggtttgatag gccacggtga cgttcaccgc
     4501 ctgtgcctga aaagcgatga atgccagtgc ggctagaagt gtgttacgcg atgaaattgc
     4561 catgattgtc tgctcccctg tcttgttatg ggagcagtat tcaggaataa aaacattcat
     4621 taaaagaatt agtcgttatc gcacagatga ttttattctt agcaaaaaaa cggctaagga
     4681 aagattaaat atgcaattac tccgttctaa caattgggaa aattactaca cttcatttta
     4741 gtgatgcata aacaaccaca ttacgagatt tattacttca ttatttctat caatatcaat
     4801 acatataata aaactattat attcgcattg tatatatcac tgtatcattc tcaatcactc
     4861 gcatattaca ttcacataat gtatataaac aatgcttgtt cacag
//
LOCUS       DAFNBJ010000251         4817 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.251, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000251 DAFNBJ010000000
VERSION     DAFNBJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.251"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..933)
                     /locus_tag="L2X49_004694"
     CDS             complement(<1..933)
                     /locus_tag="L2X49_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBN4799661.1"
                     /translation="MGKSKKNRAVSTNQIQHKSQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPNNVGGFGDVEKASKVFV"
     gene            complement(933..2390)
                     /locus_tag="L2X49_004695"
                     /pseudo
     CDS             complement(933..2390)
                     /locus_tag="L2X49_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
     gene            2359..2502
                     /locus_tag="L2X49_004696"
     CDS             2359..2502
                     /locus_tag="L2X49_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005135182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late control protein D"
                     /protein_id="HBN4799662.1"
                     /translation="MATLGFKSASLDDAEWTITTLTHTVSPDNGFTTSLELEVRIDDF
                     EME"
     gene            2593..2811
                     /locus_tag="L2X49_004697"
     CDS             2593..2811
                     /locus_tag="L2X49_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HBN4799663.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPIKSGQVALSL"
     gene            complement(3047..4732)
                     /locus_tag="L2X49_004698"
     CDS             complement(3047..4732)
                     /locus_tag="L2X49_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBN4799664.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
BASE COUNT         1192 a         1249 c         1239 g         1137 t
ORIGIN      
        1 gacaaagact ttactagcct tttccacatc cccaaaaccc ccaacattat tcggcattat
       61 ccccatcatt tgcggcggca cgcggtgtgc cgccatcatg tcgtcccggc tcacgttctt
      121 gatattcaaa aactcatcct tcgctgcgac ctctgacaac gggataatct gaagcccgtc
      181 ctttttgccg ttaggcgagt acataaacag gttgcggaag ttgcccggac ctttggcgct
      241 tttcatcgcg ttgcggaggt tgttcacatc ctcttggttt tgcgcggcat cggtcatata
      301 catgatgaag cctgcatggc tgccgttaat gtaatactta cggcggaaca acgtggcgga
      361 ctcgttgagc agggctgacg gaatagcaga aagataaccg ggcaggccgt agatctcctg
      421 gttgatatcc ggttccatca gatgaaagat gctgcctttc gtgaactgat acggctgggt
      481 tgtcataccg tattgcacaa accagtaggt atccaggtct aacccacgtc gggtgtattt
      541 tgccagagca ggctcaaggg cgataacttc accgaaccgg ttcgtgcgtt tctccaggta
      601 agcgttacca aataccagat agtcctgcac aaaacgtgaa aaagcctgct ggctgagaag
      661 cgggtgaggg atgtaggtac tggtcagaat gttgcatttc accgcaattg gtgaactgtg
      721 atgcacggca gcgcggaagg ttcgcgccag tccgtcaaaa ctcactggcg gctcatacca
      781 gcgatctgtc tgtacgcatt ccacatagtc cagcagttcg cggcggtcca gaacaggaac
      841 gggatcgcca aagctgaatg cttcggctga agtctggctt ttatgctgaa tctggttcgt
      901 cgacacagcg cggttcttct tactctttcc catcaaaaaa tctccacaat attgctggta
      961 ttggcggact cgccctgcag cggctcgtta aacagtgcgt gcattgttgc ccacgccaga
     1021 tcggcatggc tggcttcttc gctgcggctg gcttcatagg tcgggcggtt gccgctggcg
     1081 gtggtggcgc gacgaattgc cataaatgac tgcgctatgt cggtgtgtcc ggcgtcaaac
     1141 tccagacggc ggtgactgat aatgtcgtag gccttgagta ccagggcatt tttaacgttg
     1201 gggttgtaga caaactcccg gacggcagga aaaaacgctt tcacgttctc gtaaaccccg
     1261 tgaccgacgc cggttgagtc gataccgatg tatgtcacgt tgtactgttc agtcagtttt
     1321 ttgatggcgt cagcctgggc gcggaagtcc atcccgcgcc actggtgacg ctcaagaatg
     1381 cggaacttac cgcccggcac ggctggcggt gccaccacca cgcatccggc gctgtcgccg
     1441 ttctgcgtac cttttgccgg gtcataaccg atccacactt cgcgccagcc aaacgggcgc
     1501 agcgccagtg catgaaagtc ggtccagact tcccaactgt ccaccatgca cgcctgtagt
     1561 tcgctgagcg ggaacacgga cgcgagatcg tccacaaact cgcacatcag caggttctgg
     1621 tattcgtccg ggctgtattc catgcgcaac tggtcaaggt cgaacaggtt acagccgccg
     1681 cgcaccgcat cttccacggt gactatctgg cggtattgcc cgtcagcgca cagcaggccg
     1741 ggggccagat tgctgtggga cagatcaatg tccaccttgt cggctttgtt gcgtccacgg
     1801 ttgaacagcg caccggacca gaacggataa gcactgtggg tcaggctgga tggtgtggaa
     1861 aaataggtct gccgccattt cttgtgaata gccataccgg aagccacttt gcgtagctcc
     1921 tgaaatttcg gtatccagaa atattcatcc agatacaggt tgccgtgata actctgggcc
     1981 gtgcgggcat tggtgccgag gaagtaaagc gtggccccgt taggaagcac catcggatcg
     2041 cctttcagtt ccacatccac ttctttggcg aagtcgatga tgtactgctt aaagacgtgg
     2101 gcctgtgcct tactggcgga aaggaaaatc tggttacgtc cggtaagcag ggcgtcaatc
     2161 agggcttcac gggcaaagta aaaggtcgcg ccgatctggc gtgacttcag caggttgcgg
     2221 atgcggttgg tttttccggc ttcccaccag tggcgctggt agttgaacat ggaggaatgg
     2281 aagatttctt ccagcttctc aatctgttca tcggtgaaga cattcttttc cggctgacgg
     2341 cgcgggcctt tgttgcggtt ggcgacgtta gggtttaagt cagcttcgtt agatgatgcg
     2401 gaatggacca ttacgactct gacgcatacc gtcagcccgg ataacggttt tacgaccagt
     2461 ctggagcttg aagtgaggat tgatgatttc gaaatggaat gattcttcgc aatggagaac
     2521 ttttaagttt gcaaaatgga ataatgcggt atcattattg tgaatttagc aaaaatgggg
     2581 agaactcgaa aaatgatgat ttgcccactg tgtggaagtg ccgcccatac tcgcagcagt
     2641 tttcaggtat cttcattgac caaagagcgt tacaaccagt gccagaacat taactgcagc
     2701 catacttttg ttacccatga aacttttgtt cgttcgattg caacgccaaa agagtcaaat
     2761 ccggttcagc cgcatccaat taaatcagga caggtggcgc tctctctttg acgccgccgc
     2821 cattttgtcg ccatcgttaa aaaacagttt ttctaacatc atgattttaa acagcttaaa
     2881 tttcaggcaa caaaaaaccc atcaaccttg aaccgaaatg gcggggttga tgggctccac
     2941 aaaatgggga catcaaagaa aagcagtggc actaattaag actgatgccc tgcggaaaag
     3001 ttctgcggtt gtgcaaaaaa atttcatttt cagggcaact tcagttttat cctaatcctg
     3061 gccataccat gacgatgatt gtccctgcca gcgtcagcag gacgttggcg attgcgtagg
     3121 tgcccgcata gcccagcgca gggatgttac tgcgagctgt atcactgatg atctccattg
     3181 ccggcgcgca ggtgcgtgcg cccatcattg cgccgaacaa cagtgcgcgg ttcattcgca
     3241 atacataagc accgaacaag aaacagataa ccacgggcac aagactgaca attaatccgg
     3301 caatcaacat ctgaccgcca atcgcgccca ggccgttatt aataccgcta ccggcgctca
     3361 gaccaacgcc tgccataaac accatcaagc cgaactcttt caccatgctt aatgcacctt
     3421 gcggaatgta accgaaggtc gggtggttag cacgcataaa gcccagcata attccggcga
     3481 ataacaaccc ggcagcgttc cccatgccga aactgaatgt gctgaactgg aaggtgatca
     3541 tcccgatcat cagcccaata acaaagaagg cgcagaatgc cagcaggtca gtgacctggc
     3601 tgtgaatcga gataaagccg atgcgatcgg cgatggtttt tacgcggcgg gcatcgccgc
     3661 tgacttgtaa aacgtcacct ttgttaagca cgacgttgtc atctatcggc atctcaatct
     3721 ggctacgaat gacgcggtta aggaagcaac cgtgatcggt caacttcagt tgtgcgagac
     3781 gtttacctac agcgttatgg tttttaacga ccacttcttc agtgacgata cgcatgtcga
     3841 gaaggtcacg atcgaaaact tctttaccgt tacggaagct gggatcgagt cgggcatggg
     3901 cgtcgggata gcctaccaac gctatttcat cgcccatttg tagcacggca tcaccgtctg
     3961 gatttgccag aatcccgtta cgtcgaatac gttcaatgta gcagccggtt tgtcgataaa
     4021 tacccagttc acgcagattt ttgccgtcgg tccaggccac cagttccggg ccgacgcgat
     4081 aggcgcggat caccggtaaa taaaccttac ggttggcatc agtgtccagg ccacgttcgc
     4141 gggcgatttg ctgggcgctg gtctgtaagt cctgatgctg caatttcggc aagtaacgcg
     4201 caccaacaat caaactcacc agaccgatta aataggttaa ggcatacccg aggctcagat
     4261 tatccagtgc cagtgagagc tgcctgcttt ccatgccgga atgacgcagt gtatcgccag
     4321 caccgaccag aaccggtgtc gacgtcatag agcctgctaa cataccggcc gtcaggccaa
     4381 tatcccagcc aaacagctta cctaacccta aggcgatcac cagcgcactg ccaaccatca
     4441 ccagtgctaa cattaggtaa tttttcccat cgcgaaaaaa aatggaaaaa aagttcggtc
     4501 cggcttcgac cccgacgcag aaaataaaca gcataaagcc aagattaagc gcatcggtgt
     4561 taatgctgaa atgttgttgg cctaataaca gcgatacgac taaaacgcca atggaattac
     4621 ccagttggat cgaaccaagt cgtaactttc cgagacatag cccaagcgcg aggaccacaa
     4681 ataataacag aatgtaattc ccatttaaca attcggcgac gtttatattc acggaggcta
     4741 acttcttgtt tactagtaag ctgttgaaag aaatggtaat ttacgataat gttttttacc
     4801 agaattcagg gcgcaga
//
LOCUS       DAFNBJ010000252         4695 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.252, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000252 DAFNBJ010000000
VERSION     DAFNBJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.252"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            7..789
                     /locus_tag="L2X49_004699"
                     /pseudo
     CDS             7..789
                     /locus_tag="L2X49_004699"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            964..1338
                     /locus_tag="L2X49_004700"
     CDS             964..1338
                     /locus_tag="L2X49_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799665.1"
                     /translation="MIPHRENHLVLDIANSESETELQGNRQIIAPYRGAVSYVQFTTD
                     QRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDE
                     QNLFCTITFQHVIDENKTYICQ"
     gene            1351..2309
                     /locus_tag="L2X49_004701"
                     /pseudo
     CDS             1351..2309
                     /locus_tag="L2X49_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(2348..3247)
                     /gene="nhaR"
                     /locus_tag="L2X49_004702"
     CDS             complement(2348..3247)
                     /gene="nhaR"
                     /locus_tag="L2X49_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBN4799666.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3313..4479)
                     /gene="nhaA"
                     /locus_tag="L2X49_004703"
     CDS             complement(3313..4479)
                     /gene="nhaA"
                     /locus_tag="L2X49_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBN4799667.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1419 a         1070 c          983 g         1223 t
ORIGIN      
        1 taatcgggta cgattttatc cgataactat aatgctataa ctctgggaaa tggttggaat
       61 acaccgctgg gtgccatctc tttttacgct gcccgatcga gtagtaagtt aaataatgat
      121 accagacacg aaggtacgag ctatcaggtc gcctataata aatatctagt acaaacagcc
      181 actcgattta gcgtggccgc ctggcgttat gcttcgcagg attacaggac attcagcgac
      241 catctttacg aaaatgataa acactatcat cagagtgatt atgatgattt ttatgatatt
      301 ggcagaaaaa atagcctttc tgctaatatt atgcaacctt tatccaataa tctgggaaat
      361 gtatcattaa gtgcgctttg gcggaattac tgggggcgaa gtggaaatgc taaagattac
      421 caattcagtt actccaataa ctggcaacac attagttata ctttctctgc cagccaatct
      481 tatgataaaa ataataaaga agaggagcgt tttaatctgt ttatctccat tcctttctac
      541 tggggggatg atattgccaa aacacgtcac caaattaact tatcgaattc gacctcattt
      601 tccaaagatg gctattcctc caacaatact ggaattactg gcatagccgg tgaacatgat
      661 cagttaaatt atggtatata tgttaatcag caacaacaaa ataatgatac ctcgcttggt
      721 acgaatttaa gctggagaac tcccatcgcc ataatagatg gcagctatag tcattctaaa
      781 aacgcctagc aaagtggtgg aagtattagt agtggattag ttgtctggcc cggtggtatt
      841 aatatcacta accagttatc cgatacattt gcaattctgg atgcgcctgg gttagaaggt
      901 gcacatatta atggacaaaa atacaatcga acaaacagca aaggccaggt tgtttacgac
      961 ctgataatac ctcatcgtga aaaccatctg gtacttgata tagcaaacag tgaaagtgaa
     1021 acagaattgc agggcaatcg tcaaattatt gcaccttacc gtggagcagt ttcttatgtg
     1081 cagtttacaa ctgaccaacg taagccctgg tatatacagg cactgcgtcc cgatggttcg
     1141 ccattaacct ttggctatga cgtactggat ctccaggaaa ataatattgg agtcgttggc
     1201 cagggtagtc gcctttttat tcgcgtagat gaaattccca ctggaataaa agttgctctc
     1261 aatgatgaac agaacttatt ctgtactatt acttttcaac acgttatcga tgaaaacaaa
     1321 acatatatat gccagtaaaa ggagtttact atgaagtggc ttttattaat caccttgagt
     1381 ctatattctt ttattgtgca aagtgctccc tgtgcattga ccaatgtcgg tgaacaacgt
     1441 ggaacatata tactcaagcc actgagtatg aaaggtaatc taaccgccga aaaattgaaa
     1501 acaccggaaa tatcattgag tgtacagact tgcaagagac aaccaacgcc attaaagttc
     1561 attttttaac acagaacgct ttaaggttta aagtcggaag tcgtcattat attattaatt
     1621 tcccctttga atctgacggt aaaaaaatag agcttccagg gaagtcatct tataatttag
     1681 aagaaatgct atctgcgata agtttcacca ttccatattc tatcgcatct gtatcagata
     1741 gttctaacac cgcagacatt tttcctggaa ctccttttcc tttgttgttc gaaattgaag
     1801 tgagttcatg tacagggaat aatactaatt gtaatagcgt ccgctttaat tataatttaa
     1861 atactatttt acaggtaaat ttaatgacct gtggttttga agatcaatcc atcgacactg
     1921 gacgatttgt actgtctcgt atcaataatg aaaattatca gtttcatcat ataagatttg
     1981 actgcatcca gggcgagcaa ggtgaattag tttttgaacc cagtgatgtt gaatattact
     2041 ttgaaccagt gacgatacag gagtcaggaa ccagcatatt aaaaaatgag ttagaaaaca
     2101 gtgaggacgg tgcgggtcaa ataggctttc aactctccaa tgatggtacg aatgaaatcc
     2161 agtatggtaa aagcaattac tactcttttc agcatcccca tgaaggaagt aatcagattc
     2221 ctctatttat tcgcccgcgt acatatggaa ataatgtctc aagcgggcaa ataatgagcc
     2281 gagtcaaaat cgtggttatg tacaactaat ctatctcccc caccttaact ttggggggag
     2341 gtgccgatta acgcgccgct ggactaaaaa gcgcagaata atccgtattg cagattcgct
     2401 gtaccgccgg gtgctgaatc atccgctcag caaaaatagc atggtactct tccatcacat
     2461 tctcgacgcg accaatttct acgacagttt tatcggcata aaagtcatat gcataaagcg
     2521 ttggggcaac gaagattgca ttgtgcatcg caccaaaagc tttcatcaac gcggcatcat
     2581 caaactcgcc gaggatttct acgtttaatc cctgggagtt aaaccagtta agcaatttgc
     2641 gccctaacat tgaacgtcgc ccaggaatca aaagtcgccg ttcttccaga caagccggga
     2701 acggtttttc tggtggtgga tttgtacacc agaaactcac gccacattcg ccaattctca
     2761 cggagaacag gccttcctgc tgcgtagagt ctatcggaca gtcagaaatg atcatatcca
     2821 gtttatgctg acttaattgc tccagcagca tttcgtgggt ggattcgaag cagcgaagat
     2881 gaatgggctc gccttctacc actgcggcgt taagtacgct actgaccagg cgtttggaaa
     2941 gtgcatcagc cacaccaacg tcaaacaata aattggattc tttgcgatag ttcacaatat
     3001 ccagcatttc ctggcttaag gtgaacattt tatcggcata gcgatagacc agttctccca
     3061 gctcgctggg ttcgagacca cgtcccttgc gtttaaataa tttgccttgc aggcgctctt
     3121 ccagcgctcg aatctgtccg gtaatggttt gtggagttaa ataaagcgcc tccgctgcgc
     3181 caaccacgga accttcttta tagacatgcc agaagtaata caagtggttg taattgatat
     3241 gagacatgct catttctctc cctgataaca atgaaaaggg agccgtttat ggctccccgg
     3301 taaaccgtcc tgtcaaactg atggacgcaa acgaacgcgt aaccagctgt atccaattac
     3361 cgccgaagag atagaaccga ccaggatacc gagtttcgcc cagttaatca gttctggatc
     3421 tacgctacca aaggccaggc tggcaataaa gatagacata gtaaaaccga taccgcacag
     3481 gatccccacc gccataattt gctgataagt cgttccctca ggcagatgcg ccagtttcaa
     3541 acgcagcgcc aaccagcaga acagactaat ccccagcggt ttgccaatca gcaagccagc
     3601 gatgatcccc aatggcagaa tggaggtcaa gccatccagc gtgacacctt gcagtgaaac
     3661 gccagcatta gcaaatgcaa acagcggcaa aatcagatac gccacccacg ggtgtaacac
     3721 atgctccagt cgcttcgctg gagaacgccc atgcttctct ttcaaaggaa taaagaagcc
     3781 gacaattacc cccgccaggg ttgcgtgaac ccccgatttc aacaccgcag tccacaacac
     3841 tacgccaacc agaatataga cgcccgtgcg gcgtacacca cacagattca ataccgcgag
     3901 taccgcaatt gctacagccg cgacgccaag agaggccatc gataagtcat tagtgtagaa
     3961 caatgcgatg ataatgatgg ccccaagatc gtcgataata gccagagcca tcaaaaagat
     4021 cttcagcgct aacggaacac gacttcccaa cagcgccagc acaccaagtg caaaggcaat
     4081 gtcagtagcc gccgggatcg cccacccttc gcgggtaatc ggatcggcat agttaaaggc
     4141 cagatagagt aatgccggaa caatcatccc accaatagcg gcgataactg gaaatgcggc
     4201 ctggcgtaag ctggctagcg atccttgcat cagttcacgt ttaacttcca gaccgactaa
     4261 caggaaaaat accgccatca gcgcgtcatt tatccataac agcatgtttt tgttgatttc
     4321 gagtgaacca acccggagct gaaccggcgt ctccagaaag tcgtgatacc atccactggt
     4381 tgcgccgcta ttggccataa tcatcgccag gatagcggca atgataagaa taatgcctcc
     4441 cgaggcatca ctgctaaaga atcgatgcag atgtttcact ttttatttct ctttcaggtg
     4501 aatagatcgt ttttactatt ttaccccgca caaatcatcg ttaaaatcag attatagtgt
     4561 aggaataact cgttttttac gatccattga agaatttgcg ccatcagcgg tgagatgagt
     4621 ttttgacggc tgtaagcgct cacgagtgac cctaagccca gataatcata gcttaaggtg
     4681 aagatttctg agaga
//
LOCUS       DAFNBJ010000253         4691 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.253, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000253 DAFNBJ010000000
VERSION     DAFNBJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.253"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            38..1954
                     /gene="mngA"
                     /locus_tag="L2X49_004704"
     CDS             38..1954
                     /gene="mngA"
                     /locus_tag="L2X49_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBN4799668.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPADIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            1972..4605
                     /gene="mngB"
                     /locus_tag="L2X49_004705"
     CDS             1972..4605
                     /gene="mngB"
                     /locus_tag="L2X49_004705"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBN4799669.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLSERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         1137 a         1110 c         1309 g         1135 t
ORIGIN      
        1 ctggcgcgac tataaaaacg atcaagtgag gatcatgatg aatctgacga ctctgaccca
       61 ccgcgatgcg ttgtgtctga atgcgcgctt taccagccgt gaagaggcca tccacgcgtt
      121 gactcaacgt cttgctgctc tggggaaaat ttccagtact gagcaatttc tggaagaagt
      181 gtatcgccgt gaaagccttg gcccgaccgc cttaggtgaa gggttggctg tgccgcatgg
      241 caaaactgct gcggtaaaag aagcggcgtt tgcggtcgcc acactcagcg agccgcttca
      301 gtgggaaggc gttgatggcc cggaagcggt tgatttagtg gtgctgctgg cgattccccc
      361 caatgaagcg ggtactacgc atatgcaact gctgacagcg ctgaccacgc gcctggcgga
      421 tgatgagatt cgggcgcgta tacagtcggc gacgacgcct gatgagttgc tctcggcgct
      481 ggatgacaag ggaggcacgc aaccttctgc ctctttttcc aacgcgccaa ctatcgtctg
      541 cgtaacggcc tgtccggcgg atattgctca cacctatatg gctgcggaat atctggaaaa
      601 agccggacgc aaactcggcg taaatgttta cgttgaaaaa caaggcgcta acggcattga
      661 agggcgttta acggcggatc aactcaatag tgcaaccgcc tgtatttttg cggctgaagt
      721 cgccatcaag gagagtgagc gttttaatgg cattcccgcg ctttcagtgc ctgttgccga
      781 gccgattcgc catgcagaag cgttgatcca acaagcgctt accctcaagc gtagcgatga
      841 gacgcgtacc gtacagcaag atacgcaacc ggtgaaaagt gtcaaaacgg agctgaaaca
      901 ggcactgttg agcggaatct cttttgccgt accgttgatt gtcgcggggg gcacggtgct
      961 ggcggtcgcg gtattactgt cgcaaatctt cgggctacaa gatctgttta atgaagaaaa
     1021 ctcctggctg tggatgtacc gcaagctggg cggcgggctg ctcggaattt tgatggtacc
     1081 ggtgctcgcg gcctataccg cctattctct ggcagataaa ccggcgttag cgccaggctt
     1141 tgcggctgga cttgccgcca acatgatcgg ctccgggttt ctcggcgcgg tcgttggcgg
     1201 attgatagcc ggttacttga tgcgctgggt gaaaaatcac ttgcgtctta gcagtaaatt
     1261 caatggattc ctgacttttt atctctaccc ggtgctcggt acgttgggag cgggcagtct
     1321 gatgctgttt gtggtgggag aacctgtcgc ctggatcaat aactcgctta ccgcctggct
     1381 gaacggtctg tcaggaagta acgcgctgtt gctgggtgcc attctcggtt ttatgtgttc
     1441 ctttgacctt ggagggccag tgaacaaagc cgcttatgca ttctgcctgg gcgcaatggc
     1501 gaacggcgtt tacggcccgt atgccatttt cgcctccgtc aaaatggttt cggcatttac
     1561 cgtaaccgct tccacgatgc tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg
     1621 gaaatccacc tggctgttag ggctggcagg tattaccgaa ggggcgatcc cgatggcgat
     1681 tgaagatccg ctgcgggtta ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat
     1741 tgtcggtgcg atgaatatcg gcctttcgac acccggtgcc ggcattttct cgctcttttt
     1801 acttcatgat aatggcgcgg gcggtgttat ggcggcaatt ggctggtttg gtgcggcatt
     1861 ggtgggggct gcaatctcga ctgcaattct cctgatctgg cggcgtcacg cggttaagca
     1921 tggcaactat ctgactgatg gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagcg
     1981 gtatctcgcg ttcacatcac cccgcatatg cattgggatc gggagtggta tttcaccacc
     2041 gaagagtcac gcattctgct ggtcaataat atggaagaga tcctgtgccg actggaacag
     2101 gacaacgaat acaaatatta cgtactcgac gggcaaacgg cgatcctcga agattatttc
     2161 gcggtgaaac cggaaaacaa agaccgcgtg aagaaacagg tagaagccgg taagttgatt
     2221 atcggcccct ggtataccca gaccgatacc acgattgttt ctgcggaatc catcgtccgt
     2281 aatctgatgt acggaatgcg tgactgcctc gcgtttggcg agccgatgaa aataggttat
     2341 ttaccggatt cctttggcat gtccgggcaa ctgccgcata tctacaatgg atttggcatt
     2401 acccgcacta tgttctggcg cggatgttcg gagcgccacg gtactgataa aaccgagttt
     2461 ttatggcaaa gcagtgacgg tagcgaagtg acggcgcagg tgctgccgtt gggttatgcc
     2521 atcggtaagt acttacctgc cgacgaaaac ggattacgca aacgcctcga cagttatttt
     2581 gacgtgctgg aaaaagcgtc tgtaaccaaa gagattttat tgccgaatgg tcatgaccag
     2641 atgccattgc agcaaaatat cttcgaagtg atggagaaac ttcgcgaaat ttatcctcaa
     2701 cgaaagtttg tgatgagccg ctttgaagag gtattcgagc agattgaagc gcagcgtgaa
     2761 agtctggcga ccctgaaagg tgaatttatt gatggcaaat atatgcgcgt gcatcgcacc
     2821 atcggttcca cgcgcatgga tatcaaagtt gcccacgcac gtattgaaaa taagattgtt
     2881 aatctgctgg aaccgctggc aacgctggcc tggacgttgg gttttgaata ccaccacggc
     2941 ttgctggaga aaatgtggaa agagatctta aaaaatcatg cccacgacag tatcggttgt
     3001 tgttgcagtg acaaagttca tcgcgaaatt gtcgcccgct tcgaactggc tgaagacatg
     3061 gcggataatc tgctgcgttt ctacatgcgc aaaattgccg ataacatgcc acagagtgat
     3121 gccgacaaac tcgtgctgtt taacctgatg ccctggccgc gggaagaagt tattaacacc
     3181 actgtgcggc tgcgcgccag ccagtttaat ctacgagacg atcgcggtca gcctgtaccg
     3241 tattttattc gccatgcccg tgagatcgat ccaggcctaa tcgatcggca aatagttcat
     3301 tacggtaatt acgatccctt tatggagttt gatatacaga tcaaccagat tgtcccttct
     3361 atgggctatc gcacgcttta tatcgaagcg aatcagcctg gcaacgtgat tgcggcaaaa
     3421 agtgacgctg aagggatact ggaaaatgct ttctggcaaa ttgcgctcaa tgaggatgga
     3481 acgctacaac tggtagataa agatagcggt gtgcgctatg accgggtgtt gcaaattgaa
     3541 gaaagctctg atgatggtga tgaatatgac tattcacccg caaaagaaga gtgggtaatg
     3601 acttctgcaa cagcgaaacc gcaatgcgag attactcatg aggcctggca gagcagggct
     3661 gttatccgct atgacatggc ggtgccgctc aatttgtcag aacgcagcgt ccggcaatcc
     3721 acgggcagag taggggtgga gatggttgtc actctgagtc ataacagcag acgtattgat
     3781 gtggatatca accttgataa ccaggctgac gatcatcgcc ttcgcgtcct gatccctaca
     3841 ccttttaaca ccgacagtgt tctggcagat acgcagtttg gttcgctaac gcgccccgtg
     3901 aacgacagtg caatgaacaa ctggcagcaa gaaggctgga aagaagcgcc ggttccggta
     3961 tggaatatgc tcaactatgt tgccttacag gaagggcgta acggcatggc tgtctttagc
     4021 gaagggctac gtgaatttga agtcatcggt gaagagaaga aaacctttgc cattacgttg
     4081 ctgcgtggcg tgggcttact gggcaaagaa gatctgcttt taaggcctgg gcggccttcg
     4141 ggaattaaaa tgccagtccc ggactcacaa ctacgtggtc tgctttcttg tcgcctaagt
     4201 ttattgagtt ataccggtac gccaaccgcc gctggtgtag ctcagcaggc gcgagcatgg
     4261 ctgactccag tacagtgtta caacaaaatc ccatgggatg cgatgaagct caacaaagcc
     4321 ggattcaacg tgccggaaag ttatagtttg ttgaaaatgc ccccagtggg atgcctgata
     4381 agcgcactta agaaagctga agaccgacaa gaagtgattt tacggctgtt taatccggct
     4441 gaatcagcaa cctgtgatgc gactgttgct ttcagtcgcg aggtgatttc ttgctcagaa
     4501 acgatgatgg atgaacacat taccaccgag gaaaatcaag gttcaaatct atcggggcct
     4561 tttttacccg gccagtcacg gacgttcagt taccggcttg cctgaatagc aatcaaaccg
     4621 aagccacata tgcgcggcca gattgttgac aaagggcgct ttgttcatgc cggatacggc
     4681 atgaacgctt t
//
LOCUS       DAFNBJ010000254         4667 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.254, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000254 DAFNBJ010000000
VERSION     DAFNBJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.254"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            83..1186
                     /gene="proB"
                     /locus_tag="L2X49_004706"
     CDS             83..1186
                     /gene="proB"
                     /locus_tag="L2X49_004706"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBN4799670.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1198..2451
                     /gene="proA"
                     /locus_tag="L2X49_004707"
     CDS             1198..2451
                     /gene="proA"
                     /locus_tag="L2X49_004707"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBN4799671.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPVGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MEESGVALHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2566..2641
                     /locus_tag="L2X49_004708"
     tRNA            2566..2641
                     /locus_tag="L2X49_004708"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2599..2601,aa:Thr,seq:cgt)
     gene            2805..4013
                     /locus_tag="L2X49_004709"
     CDS             2805..4013
                     /locus_tag="L2X49_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN4799672.1"
                     /translation="MKLNARQVDAAKPREKAYKLADGAGLYLEVVPSGSRYWRMKYRF
                     NGKEKRMAFGVYPAVSLAQARALRDDAKKKLAEGIDPSLAKKEEKLVRDVQLNNTFQA
                     VALEWHGTKVSRWSEGYASDIIEAFNKDIFPYIGQQPVNEIKPLVLLNVLRRIESRGA
                     TEKAKKVRQRCSEVFRYAIVTGRAEYNPAADLTSAMSGHESKHYPFLTVEELPDFFKA
                     LAGYTGSPLVVLAARLLILTGVRTGELRGAFWSEFDLEKAVWEIPAERMKMKRPHLVP
                     LSTQALEIVQQLKVMSGQYPLVFPGRNDPRKTMSEASINQVFKRIGYTGRVTGHGFRH
                     TMSTILHEEGFNTAWIETQLAHVDKNAIRGTYNHALYLEGRREMMQWYADYINANHNS
                     CISLLVNATK"
     gene            4255..>4667
                     /locus_tag="L2X49_004710"
     CDS             4255..>4667
                     /locus_tag="L2X49_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBN4799673.1"
                     /translation="MNSNYMPFTQRDSLGRYYTKESISALLVSQMKAEKVNNIIDLAS
                     GEGSLTYAALDRWKNAEAYSLDIESRMSKKVCDNLTHIVTDALVHSFPEMLARHQGNF
                     DVAVCNPPFTLPEWRDDYFKIISEIGADKYISVSK"
BASE COUNT         1191 a         1065 c         1265 g         1146 t
ORIGIN      
        1 tgcagatggt tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc
       61 taaaattgaa tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac
      121 cagtgtgcta acaggcggat cgcgccgtct gaaccgtgcc catatcgttg aacttgttcg
      181 ccagtgcgcg cagttacatg ccgccgggca tcggattgtt attgtgacgt cgggcgcgat
      241 cgccgccgga cgtgagcacc tggggtaccc ggaactgcca gcgactatcg cctcgaaaca
      301 actgctggcg gcggtagggc agagtcgact gattcaactg tgggaacagc tgttttcgat
      361 ttatggcatt cacgtcgggc agatgctgct gacccgtgct gatatggaag accgtgaacg
      421 cttcctgaac gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat
      481 caatgagaac gatgctgtcg ctacggcaga gattaaggtc ggcgataacg ataacctttc
      541 tgcgctggcg gcgattctgg cgggtgccga taaactgttg ctgctgaccg atcaaaaagg
      601 tttgtatacc gctgatccgc gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg
      661 cattgatgac gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg
      721 catgagtacc aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat
      781 tgccgcgggc agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac
      841 gctgttccat gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc
      901 gccggcgggt gaaatcacgg tagatgaagg ggcaactgcc gccattcttg aacgcggcag
      961 ctccctgttg ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat
     1021 ccgcatttgc aacctcgaag gtcgcgatat cgcccacggc gtcagtcgtt acaacagcga
     1081 tgcattacgc cgtattgccg ggcaccactc gcaagaaatt gatgcaatac tgggatatga
     1141 atacggcccg gttgccgttc accgtgatga catgattacc cgttaaggag caggctgatg
     1201 ctggaacaaa tgggcattgc cgcgaagcaa gcctcgtata aattagcgca actctccagc
     1261 cgcgaaaaaa atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc
     1321 atcctcaacg ctaacgccca ggatgttgct gacgcgcgtg ccaatggcct tggcgaagcg
     1381 atgcttgacc gtctggcact gacgcccgca cggctgaaag gcattgccga tgatgtgcgc
     1441 caggtgtgta acctcgccga tccggtgggg caggtaatcg atggcagcgt actggacagc
     1501 ggcctgcgtc ttgagcgtcg tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg
     1561 cgcccgaacg tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa cgcagtgatt
     1621 ctgcgtggcg gtaaagaaac ctgtcgcact aacgcggcaa cggtggcggt gattcaggac
     1681 gccctgaaat cctgtggctt accggtgggt gccgtgcagg cgattgataa tcctgaccgt
     1741 gcgctggtca gtgaaatgct gcgtatggat aaatacatcg acatgctgat cccgcgtggt
     1801 ggcgctggtt tgcataaact gtgccgcgaa cagtcgacga tcccggtgat cacaggtggt
     1861 ataggcgtat gccatattta tgttgatgaa agtgtagaga ttgctgaagc attaaaagtg
     1921 atcgtcaacg cgaaaactca gcgtccgagc acatgtaata cggtagaaac gttgctggtg
     1981 aataaaaaca tcgccgatag cttcctgccc gcattaagca aacaaatgga ggaaagcggc
     2041 gtggcattac acgcagatgc agctgcgctg gcgcagttgc agacaggccc cgcgaaggtg
     2101 gtggctgtta aagcggaaga gtatgacgat gagtttctgt cattagattt gaacgtcaaa
     2161 atcgtcagtg atcttgacga tgccatcgcc catattcgtg aacacggcac acaacactcc
     2221 gatgcgatcc tgacccgcga tatgcgcaac gcccagcgtt ttgttaacga agtggattcg
     2281 tccgctgttt acgttaacgc ctctacgcgt tttaccgacg gcggccagtt tggactaggt
     2341 gcggaagtgg cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg gctggaagca
     2401 ctgaccactt acaagtggat cggtattggt gattacacca ttcgtgcgta aataaaaccg
     2461 ggtgatgcaa aagtagccat ttgattcaca aggccattga cgcatcgccc ggttagtttt
     2521 aaccttgtcc accgtgattc acgttcgtga acatgtcctt tcagggccga tatagctcag
     2581 ttggtagagc agcgcattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc
     2641 atttcaaact cttcccaagt ctaccgaaat caactaaaag ctcgtatact gcggtttctg
     2701 gcccgtatct tatctcctgt tatcaactag actcaaccga aatcaagtcg cagttggggg
     2761 cataagtggg ggcattttgt gttcggtcta gggagatgcc cccaatgaaa cttaatgcgc
     2821 gacaggtaga cgcagctaaa cccagagaga aagcctataa gctggcagat ggtgctggtt
     2881 tgtatcttga ggttgttccc tctggttcca gatactggcg gatgaaatat cgcttcaatg
     2941 gaaaagagaa gcgtatggcc tttggtgtct atccggcagt gtcccttgca caagcgaggg
     3001 cactgcgtga cgacgccaag aaaaagcttg ccgaaggtat cgatccatcg cttgccaaga
     3061 aagaagaaaa gctggttcga gatgtgcagc taaataatac gtttcaggct gtggcacttg
     3121 aatggcatgg aacgaaggtg agccgatggt cagaaggtta tgcctcggac attatcgaag
     3181 cctttaataa agatattttt ccctatattg gccaacaacc ggtgaatgaa atcaaaccgc
     3241 tggttctgct taatgtgctg cgtcgaattg aaagccgtgg cgcgacagag aaggccaaga
     3301 aggttcgcca gcgttgcagc gaagtctttc gttacgccat cgtaaccggt cgtgcggaat
     3361 acaatcctgc agcggatctt accagcgcga tgtcagggca tgaatcgaag cattatccct
     3421 tccttactgt tgaggagtta ccagactttt ttaaagctct cgcaggctac acaggaagcc
     3481 cgttagttgt tcttgcggca cgtctgctga tcctcacggg agttcgcact ggcgaactcc
     3541 gaggtgcttt ctggagtgag tttgatcttg aaaaagcggt gtgggagata cctgccgagc
     3601 gtatgaagat gaaacggcct caccttgtcc ccctctctac ccaagcgctg gaaatcgtac
     3661 agcaactcaa agtgatgtca gggcaatatc cactggtgtt cccgggacgt aatgatcccc
     3721 gcaagacgat gagtgaagcg agtattaatc aggtttttaa gcggattgga tatacgggga
     3781 gggtaacggg gcatggtttc cgtcacacga tgagtacgat tttgcatgag gaaggtttca
     3841 atacagcgtg gattgaaacc cagctggctc acgttgataa aaatgcgatt cgtgggacgt
     3901 ataaccatgc gttgtatttg gaaggacgta gggagatgat gcagtggtat gcggactata
     3961 taaacgctaa tcataattca tgtatttctt tgctggtgaa cgctacaaag tagatttgac
     4021 gtgatgatag gattgattag gaaaatgacc taaaaccgta tattttgata cttaagttta
     4081 atcattagcc ctatatccga tcgccctttg ttacctacac ttctatcttt tttaggttct
     4141 agggctgaaa gatttgaaga taagtttata aatatcaata gattacatga ttgtgttggt
     4201 tcttgggtag ccgtaacgcc tagggcggta gcgtacttaa gatgagtata aaaaatgaac
     4261 agtaactata tgccattcac acaacgagat agtctcggaa gatactatac aaaagaatcc
     4321 ataagtgcat tattggtttc tcagatgaaa gctgaaaaag tcaataatat tattgattta
     4381 gcttctggtg aggggagctt aacctatgct gctttagacc gatggaagaa tgcagaagca
     4441 tattctttgg atatagaatc tcggatgtca aaaaaagtat gtgataatct tactcatatt
     4501 gtaacagatg cccttgttca ttcatttcct gaaatgcttg cccgacatca aggaaatttt
     4561 gatgttgcag tatgtaatcc tccattcact ctccctgaat ggagggatga ttattttaaa
     4621 atcattagtg agattggcgc agataaatat atatctgtct caaaata
//
LOCUS       DAFNBJ010000255         4423 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.256, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000255 DAFNBJ010000000
VERSION     DAFNBJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.256"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(19..201)
                     /locus_tag="L2X49_004711"
     CDS             complement(19..201)
                     /locus_tag="L2X49_004711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799674.1"
                     /translation="MKFILMFFILAILIWGFTGLTFKDAILFTSVGLSISVVALSALL
                     VVALTFVMAAMYAAHG"
     gene            complement(288..536)
                     /locus_tag="L2X49_004712"
     CDS             complement(288..536)
                     /locus_tag="L2X49_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799675.1"
                     /translation="MTSNRCREIKTPPEHIIADYILRFMKNNKEAKLYEAMSRLESKI
                     KLFVADGHDELHIRALLNQAAHSHTKESLKQACERICN"
     gene            complement(574..795)
                     /locus_tag="L2X49_004713"
     CDS             complement(574..795)
                     /locus_tag="L2X49_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799676.1"
                     /translation="MDKYLALDRALNEILTPVPTPFSTLFAGEIKAECHRIAAGESNP
                     QPSHILDRRLLALCKAGQINYTTGKGWLK"
     gene            complement(971..1324)
                     /locus_tag="L2X49_004714"
     CDS             complement(971..1324)
                     /locus_tag="L2X49_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799677.1"
                     /translation="MSLLRLTDISMDIYDMTEKEVRMDEDKVLSFINSGDGDAGDLLD
                     DAMPTAARRFYRLTQSMHTLLGEVREHFPDAMFYSANGKVSLLLGSSHNKHDQPMQEM
                     VAVSATQLHIEGGDW"
     gene            complement(1340..1948)
                     /locus_tag="L2X49_004715"
     CDS             complement(1340..1948)
                     /locus_tag="L2X49_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799678.1"
                     /translation="MFLHLVPKILHPMGNLCTLDSVSVPELSLRLTGNDLVAMRPYPN
                     KQYLVGMLKGRRALNGFLVKSPRAFEEFTMVSVWNIEGFGKITHTLKTFVEDTDYDLV
                     SHDVLLAQGSYRAQASEQCRVHPVYKNIAPVHIEPKMESLLSTEPNFENDVCETHSWG
                     MLVRSRDEGFKAMTMPSARLQESVALRGDRQPQLEQAIVITG"
     gene            complement(1979..2503)
                     /locus_tag="L2X49_004716"
     CDS             complement(1979..2503)
                     /locus_tag="L2X49_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBN4799679.1"
                     /translation="MKVLNNKGAVIELPNFSELLPKVESDDGRFSKPKNKISKEQRAE
                     LRLKFGGRCAYCGCPLPEKGWHADHVEPVRRDFEMVRAPAGSRVTHRARSTGKVMHPE
                     LHAIENLFPACAPCNLFKGALSVEGMRKEISRQVERARAYSVNFRTAERFGLIEVTEK
                     PVVFWFEIHQATAQ"
     gene            complement(2534..2845)
                     /locus_tag="L2X49_004717"
     CDS             complement(2534..2845)
                     /locus_tag="L2X49_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799680.1"
                     /translation="MNNEMSSLGTQKAEGPFVLLTLVGEGFIFDERHEIVRINGRPKQ
                     IGVKRNYFESDLGEGKAKYWTLDINEAHVFPTLDAATEQLCKLSRPHLIKIRKLIQEN
                     K"
     gene            complement(2842..3540)
                     /locus_tag="L2X49_004718"
     CDS             complement(2842..3540)
                     /locus_tag="L2X49_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799681.1"
                     /translation="MKTHGMLFNDEMVRAILADQKTQTRRIIKPQYSSDEWSIRPAQT
                     PRHRGHTHDWWLPTGTQPYSALRPCPYGVVGDRITVREAFSLLGNEDACAVDWNDNIV
                     MDRTEAARIYRASCEQRSGDYGLWSIPDEADWKPRTENMKFEGAWTPSIHMPRWASRI
                     ALEITDLRVERLNTISEEDAKAEGAPTECCVLGDKHFLGFRSLWKNTYGEDSWQANPW
                     VWVVNFKRITGEVV"
     gene            complement(3681..3905)
                     /locus_tag="L2X49_004719"
     CDS             complement(3681..3905)
                     /locus_tag="L2X49_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799682.1"
                     /translation="MPIQEKTTVFVFNACHADKAAAASANALHSLEVEYPMTLNDLSL
                     LCESVAKALDVPGGVKYEITTEPVVDGEYD"
     gene            complement(3992..>4423)
                     /locus_tag="L2X49_004720"
     CDS             complement(3992..>4423)
                     /locus_tag="L2X49_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799683.1"
                     /translation="SKLKCIAISWHFNKPTGKEHLLLFVAIVAWIFPGLVFFKHSLWG
                     GLLFFFFVAGSYAFLAFPHFTHRKKIWKERLDQSLKEYQPLDLQAWAYFKDRVREEGM
                     TYKACESWMSTEASALYKSPKQEWSFLDSTSVGENDKSQPD"
BASE COUNT         1110 a         1148 c          960 g         1205 t
ORIGIN      
        1 atcaataagt tggagtcatt acccatgagc cgcatacatt gcagccatga caaatgtcag
       61 agctaccaca agcagcgcag acaaagccac aaccgaaatg ctcaggccga cggatgtaaa
      121 cagaatggca tctttaaaag tcagacctgt aaaaccccag atcagaatgg ctaaaatgaa
      181 aaacataagg ataaatttca ctagatttgc gtccctcttt cggtggcaga gtgtcggtca
      241 ggatgcctgt ggcgaggcaa ctacagacag aatttaacac aaaaaattta attacaaatc
      301 cgttcacagg cctgtttcaa tgattctttg gtatggctat gtgcggcctg atttaacaat
      361 gcccggatgt gtagttcatc atgaccgtca gctacaaaga gtttgatttt actttcaagg
      421 cggctcatgg cttcgtagag ttttgcctct ttattgttct tcataaagcg aagaatgtaa
      481 tccgcaataa tatgctctgg tggcgtttta atttcacggc agcgattgct tgtcatgagt
      541 ttttcctcaa gtttgtggca aaatggcacc atctcattta agccaccctt ttccggttgt
      601 ataattaatt tgacctgctt tacacagcgc cagcaaacgg cgatcaagaa tatgcgacgg
      661 ttgcggattg ctttccccgg ctgcaatccg gtgacactct gctttgatct ctccggcaaa
      721 taatgtcgag aacggcgtag gaactggggt aagaatttcg ttgagtgccc tgtccagcgc
      781 cagatactta tccatattca tgtccttgca ggtaattgaa tggatggggt ttaaatttat
      841 tatactgaat cgagttacta aactaatatc cttcaggatg aagaaaggaa tcatccacct
      901 tcagtatgta ttcgacatat aggacacgtc tctaaaattt ttcgcgtttt cgcgatttac
      961 accgacaggc ctaccagtca ccaccttcga tatgaagttg cgtcgcagaa acagccacca
     1021 tttcctgcat tggctggtca tgcttgttat gactactccc aagcagtagg ctcaccttgc
     1081 cgttagcgga ataaaacata gcatcaggaa aatgctctct gacttccccc agcaatgtgt
     1141 gcatcgactg agtcagtcgg tagaatcgac gtgcggcagt cggcattgca tcatcaagga
     1201 gatctccggc gtcgccgtcc ccgctgttta tgaaactcag taccttatct tcgtccattc
     1261 gaacctcttt ttcagtcatg tcatatatat ccattgagat atctgtcagc ctgagcaacg
     1321 acatcaggct gaccttcgct cacccggtaa taacgatggc ctgttccaat tgtggctggc
     1381 gatccccccg cagcgctacc gactcctgga ggcgtgcgga aggcatcgtc attgccttaa
     1441 acccctcatc ccgcgaacgc accagcatcc cccacgaatg ggtttcacag acatcgttct
     1501 cgaaattggg ttcggtcgac agcagtgatt ccatcttcgg ttcgatgtgt accggcgcaa
     1561 tgtttttata aacagggtga acacgacact gttcagacgc ttgtgctcgg tagctccctt
     1621 gcgccagcag cacgtcatgg gaaaccaggt cataatctgt atcttcgaca aaagtcttca
     1681 gggtgtgcgt tattttgccg aatccttcga tattccagac ggacaccatc gtaaactctt
     1741 caaacgcacg gggacttttg accaaaaatc cattcagcgc cctacgcccc ttcagcatcc
     1801 ccaccaggta ttgcttgttg ggataagggc gcatcgcaac aaggtcatta ccagtcaggc
     1861 gtagcgacag ttccgggacg cttacggaat ccagcgtgca aagatttccc atcggatgca
     1921 ggatttttgg tacaaggtgc aaaaacatcg gtattctcct cgtttattaa gccttcaatt
     1981 attgtgccgt cgcctgatgt atttcgaacc agaagacaac cggcttttcc gtgacttcaa
     2041 ttaacccaaa tcgctctgcg gttctgaagt tgacgctgta tgccctggcg cgttctacct
     2101 ggcggcttat ttctttcctc atgccttcaa cacttaatgc acctttaaag agattgcagg
     2161 gggcgcaagc cggaaaaaga ttttcgattg catgcagttc agggtgcatg accttaccgg
     2221 tacttcgagc ccggtgtgtc actcgacttc cagcgggggc tctgaccatt tcaaaatccc
     2281 gcctcaccgg ttcaacatga tcggcatgcc agcctttttc cggcaaagga cagccgcagt
     2341 aggcacaccg accgccaaat ttcaaacgca gttcggctcg ttgctctttg gatattttat
     2401 tttttggttt tgaaaatcgc ccatcatcag attcaacctt cgggaggagt tctgaaaaat
     2461 tgggtaattc gattactgcg cctttgttat ttagaacttt caccggacct cccaaccata
     2521 ttaagacgat tttttattta ttttcctgaa ttagttttcg aattttaatc aggtgtggtc
     2581 ggcttaattt gcatagctgt tcggtcgcgg catccagagt gggaaataca tgggcctcat
     2641 taatatccag tgtccagtat ttcgctttac cttctcccag atcagattca aagtaattcc
     2701 gctttacccc aatctgcttt ggtcttccat taattctgac gatttcatgc cgctcgtcga
     2761 agataaaacc ctctccgacc aacgtcagga ggacaaaagg gccctcggcc ttttgcgtac
     2821 ccagcgaact catctcatta ttcatacgac ctccccggta atacgcttaa agttgacgac
     2881 ccagacccac gggttggcct gccaactgtc ctcgccgtac gtgtttttcc agaggctgcg
     2941 gaagccaaga aagtgcttat cgccaagaac acaacattct gttggtgcgc cttcagcttt
     3001 ggcatcttcc tcgctgatgg tatttaaccg ctctacccgc aaatcggtaa tttccagtgc
     3061 aatacggctg gcccagcgag gcatatggat tgaaggtgtc cacgcacctt cgaacttcat
     3121 attttcggta cgaggcttcc agtcggcctc atcagggatc gaccacagcc cataatcccc
     3181 cgaccgctgt tcgcagctcg cccggtaaat gcgggctgct tcagtcctgt ccatcacgat
     3241 gttgtcattc cagtccaccg cgcacgcatc ttcattcccg agcaatgaaa acgcctcacg
     3301 gaccgtaatg cggtcgccaa ctacgccgta cggacaaggc cgaagcgccg agtatggctg
     3361 ggtccctgtt ggaagccacc agtcatgcgt gtgcccgcga tgtcgcggtg tttgcgcagg
     3421 ccttatagac cattcatccg aactatattg aggctttata atccgccgcg tctgtgtttt
     3481 ctgatctgcc agaattgccc ggaccatttc atcattaaaa agcattccat gtgtcttcac
     3541 tgtgctgtct cctttcacac atggttcaca cattgagagg cctatccctc tttatcagcc
     3601 aggcataccg aaactgtcgg tagttttacg ggaacgggca actgcccccc tacctgataa
     3661 agtagtgttc tcccccaaaa ttaatcgtat tccccatcca ctaccggctc agtcgtaatt
     3721 tcatatttca ctccgccggg tacatccaac gctttagcga cggactcaca cagcaatgac
     3781 aaatcattca gggtcattgg gtattcaact tccaggctgt gcagtgcatt ggcgctggca
     3841 gcagcagcct tgtcagcatg acaggcatta aaaacgaaaa cggtggtttt ttcttgaata
     3901 ggcatagtgc aatcctctga ataattagcc ctgtgatcag ggcggttttt gattaaattt
     3961 aaatgttcgt tttcaataat tagtgaatct gttaatcggg ttgtgactta tcattttctc
     4021 caaccgaagt gctgtcgaga aatgaccatt cttgtttggg gcttttatat aatgcactag
     4081 cctcagtact catccacgac tcacaggctt tataagtcat gccttcctct ctaacgcggt
     4141 ccttaaaata cgcccaggct tgcagatcaa gcggctgata ctctttcagc gactggtcaa
     4201 gcctttcctt ccagattttc ttccgatgag taaaatgagg aaaagccagg aatgcataac
     4261 tacctgcaac aaaaaaaaag aacaggagtc cgccccatag agaatgtttg aaaaacacca
     4321 gtcctgggaa aatccatgcc acaatggcaa caaataaaag aaggtgctcc ttcccagtag
     4381 gtttattaaa atgccaggaa attgcaatac actttaattt act
//
LOCUS       DAFNBJ010000256         4386 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.257, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000256 DAFNBJ010000000
VERSION     DAFNBJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.257"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..199)
                     /locus_tag="L2X49_004721"
     CDS             complement(<1..199)
                     /locus_tag="L2X49_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799684.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIAL"
     gene            428..1036
                     /locus_tag="L2X49_004722"
     CDS             428..1036
                     /locus_tag="L2X49_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBN4799685.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1134..2525
                     /gene="selA"
                     /locus_tag="L2X49_004723"
     CDS             1134..2525
                     /gene="selA"
                     /locus_tag="L2X49_004723"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBN4799686.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2522..4366
                     /gene="selB"
                     /locus_tag="L2X49_004724"
     CDS             2522..4366
                     /gene="selB"
                     /locus_tag="L2X49_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBN4799687.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          984 a         1120 c         1290 g          992 t
ORIGIN      
        1 gcagggcgat gtcggtttca ccgggaagga cctttgcacc gagcagggga ttgaccggat
       61 ggccggacgt cactccgccg gggcacaccg aacaggccac gccggtgggg gcaccaatgc
      121 gcaccccggc tgaaccctga acaatgctac cgccatactg cgtcatgtcg ccctgacgcg
      181 ctgccggttt tccgctcatc tgctcatcct tgttttatcg ggttatttgt ctgcttccag
      241 gcaaaaccct acagatttat aagaaagcct taagtgagat atccatcaga tagaaacaat
      301 taggaaaata tttatatcac catgataaat atgtgattta tttggaagta acaaaccatc
      361 aacattttct gccctttttc ccatcaccag gccatactga tatttttcct cgcacaaagg
      421 actcggcatg aaactcgtcg gtagctacac cagcccgttt gtacgcaaac tttctattct
      481 gttgttagaa aagggcataa ctttcgaatt tattaatgaa ctgccctata acgcggacaa
      541 cggcgtggcg caatttaacc cgttaggaaa agtgccggtg ctggtgaccg aagagggcga
      601 atgttggttt gattcgccga tcatcgctga atatattgaa ttaatgaatg tcgctccggc
      661 gatgttgccg cgcgatccgc tggagtcgtt gcgggtgcgc aaaattgagg cactggcgga
      721 tggcattatg gatgccgggc tggtatcggt gcgtgaacag gcgcgtccag cggcgcagca
      781 gtctgaagat gaattgttac gccagcggga gaaaatcaac cgcagtctgg atgtgctgga
      841 aggatatctg gtcgatggca cactcaaaac cgatacggtc aatctggcga ctatcgccat
      901 tgcctgtgcc gtcggatatc tcaatttccg ccgcgttgcg ccaggttggt gtgtcgaccg
      961 cccacactta gtcaaactgg tcgaaaacct gtttagccgc gaaagttttg ctcgcaccga
     1021 accgccaaag gcttgatgcg cgatatgtcc tcctgaccca tctcacgtta caatccgtgg
     1081 ttatgttaaa cgcccttctc cgtgtgagag ggccttgatc agccaggttt cctatgacaa
     1141 ccgaaacgcg ttccctctat agtcaacttc cggctattga tcgcttattg cgcgatagct
     1201 ccttcctttc tttgcgtgat acttatggtc acacccgcgt ggtggaattg ttgcgtcaga
     1261 tgctcgacga agcgcgagaa gtgattcgtg gcagccagac gctgcctgcg tggtgtgaaa
     1321 actgggcgca agaagtcgat gcccggttga cgaaagaagc gcagagcgcg ctgcgtccgg
     1381 tgatcaacct gacgggaacc gtgctgcata ccaaccttgg acgagcttta caggcggaag
     1441 ccgcggtgga agccgttgcg caggctatgc gttcgccagt gaccctcgag tacgatctgg
     1501 acgacgccgg acgcggacat cgcgatcggg cgctggcgca actgctgtgc cgtattacgg
     1561 gggcggaaga tgcctgtatc gtcaataaca atgcggcggc ggtgttattg atgttggcgg
     1621 ccactgccag cgggaaagag gtggtagtat ctcgcggcga attggtggag attggcggcg
     1681 cgtttcgtat tcccgatgtt atgcgtcagg caggttgcac cctacacgaa gtagggacca
     1741 ccaaccgcac gcacgcgaat gattatcgtc aggcggtgaa tgaaaatacc gcactgttga
     1801 tgaaagtaca taccagtaac tacagcattc aggggttcac caaagcgata gatgaagcgg
     1861 aactggtggc gctcggcaaa gagctggatg ttcccgtagt gactgattta ggcagtggct
     1921 cgctggtcga tcttagccag tacggtttgc cgaaagagcc aatgccgcag gagttgattg
     1981 cggcgggcgt cagtctggtg agtttctccg gcgacaagtt gttaggcggg ccgcaggcag
     2041 gaattattgt tggtaaaaaa gagatgatcg cccgcctgca aagccacccg ctgaagcgtg
     2101 cattacgcgc ggataaaatg accctcgcgg cgctggaagc cacgttgcgt ctttatttac
     2161 accctgaagc tctgagtgaa aaattaccga ccctgcgcct gcttacccgc agcgcagagg
     2221 tcattcaaat ccaggcacaa cgtttacagg ctccccttgc cgcacattac ggcgcggagt
     2281 ttgcggtaca ggttatgcca tgtctttcgc agattggcag tggttcgctg ccggttgatc
     2341 gcctgccgag cgcggcatta acgtttacac cccatgatgg acgcggtagc caccttgagt
     2401 cattagccgc ccgctggcgt gaattgccag tgccggtgat tggtcgtatt tatgacggac
     2461 gattgtggct ggatttacgc tgccttgaag atgagcaacg gtttttggag atgttgttga
     2521 aatgattatt gcgactgccg gacacgttga ccacggcaaa acaaccttat tgcaggcgat
     2581 tactggcgta aatgctgacc gtctgccgga agaaaaaaag cgcggcatga ccatcgatct
     2641 cggctatgcc tactggccgc agccggatgg tcgcgtgcct ggttttatcg acgtacccgg
     2701 tcatgaaaag tttctttcca acatgctggc gggcgttggt ggtatcgatc acgcgctgtt
     2761 ggtggtggcg tgcgatgacg gcgtgatggc gcagacccgt gagcatctgg cgattttgca
     2821 gctgaccggt aacccgatgc tgacagtggc gctgaccaag gccgatcgcg tggacgaagc
     2881 gcgtgttgat gaggttgaac gccaggtaaa ggaggtgctg cgggaatacg gttttgctga
     2941 ggcaaaactg tttatcaccg cagcaaccga aggtcgggga attgatgccc tgcgcgagca
     3001 tctgcttcag ttaccaggac gtgaacacgc cagccaacat agtttccgcc tcgcgatcga
     3061 ccgcgcattt accgtaaaag gtgccgggct ggtcgtcacc ggtacggcgt taagcgggga
     3121 agtgaaggta ggcgattcac tctggctgac tggtgtaaat aaaccgatgc gtgtacgtgc
     3181 gctgcatgca caaaaccagc caacagaaac cgcccatgcc gggcagcgta tcgcgcttaa
     3241 catcgcgggt gatgcggaaa aagagcagat taaccgtggc gactggctgc ttgccgatgc
     3301 gccgccagag ccgttcacac gggtgattgt cgagcttcaa acccatacgc cgctgaccca
     3361 gtggcagccg ctgcatattc accacgccgc cagccacgtt acgggacgcg tttcgctgct
     3421 ggaagataac cttgccgagc tggtgttcga cacgccgctg tggctggcgg ataacgatcg
     3481 cttagtgctg cgcgatatct cggcccgcaa cacgctggcg ggggcgcgtg tggtgatgct
     3541 taacccgccg cgtcgcggta aacgtaagcc ggaatatctg caatggctgg cgtctcttgc
     3601 acgggcgcag agcgatgccg atgcgttatc tgttcatctg gaacgcggcg cggttaacct
     3661 tgcggatttc gcctgggcgc gccagctcaa cggcgaaggg atgcgcgaat tgctgcaaca
     3721 gcctggctat attcaggctg gttatagctt gttgaatgcg ccggttgccg ctcgctggca
     3781 acggaaaatt ctcgacacat tagcgactta tcatgagcaa catcgcgatg aacctgggcc
     3841 tgggcgcgaa cgtctgcgac gtatggcgtt gccaatggaa gatgaagcgc tagtactgtt
     3901 gctgattgaa aagatgcgcg aaagcggcga catcctcagt catcacggtt ggctgcatct
     3961 gccggatcac aaagcgggct tcagcgaaga gcagcaggcc atctggcaaa aagcagagcc
     4021 gctgtttggt gacgagccgt ggtgggtgcg tgatctggca aaagagacgg gtaccgacga
     4081 gcaggcaatg cgcctgacgc tacgccaggc ggcgcagcaa ggaataatta ccgcgatcgt
     4141 caaagatcgt tattaccgta acgatcggat tgtcgaattt gccaatatga tccgcgatct
     4201 cgatcaggag tgtggttcaa cctgcgcggc ggatttccgc gatcgcttag gcgtaggccg
     4261 aaagctggca attcagattc tggaatattt tgaccgcatt ggctttacgc gtcgtcgtgg
     4321 aaatgatcat ttattgcgcg acgcattatt atttccggaa aaataaggaa atgattaatt
     4381 aatttt
//
LOCUS       DAFNBJ010000257         4341 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.258, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000257 DAFNBJ010000000
VERSION     DAFNBJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.258"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            142..3159
                     /locus_tag="L2X49_004725"
     CDS             142..3159
                     /locus_tag="L2X49_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element IS3000 family transposase"
                     /protein_id="HBN4799688.1"
                     /translation="MAFEERVQILSEAEQDELYGPPAFTSADQRFFFSLNDKELAIAK
                     SLRHRGQRYMLVVLLGYFKAKPVVLNPGFHQIKQDLKYVYQTVLPGPGCRPFNLTPKE
                     NERIYQRVFQLCNYQRWNVKDHGAALRDYLSQQARAWTAPRHLFDAANEYCSGQKIAI
                     PAYSTLQKIISQVVGDEQEHMAAHLERAMSRGLKQALAELVNGTGPLPFRQLRQSARN
                     FTGTELEKELIVYRHIQHWMPEVDLLLSTLSLSQKNLQHLAEKVDYYGAKLKRQTVGS
                     QWLYLLCYLQTRWQQALERIADGFVHHVRQTKQKAKDYAQEAVFKDWQKAAKNVSKAA
                     EVLHLFIDDSIDLQLPFATVRQQALSLLTKRDLESVCLFLNEQRRSVDEAMWQYCDEK
                     ESLRKGLLRELFLCLRFEGCDGTQHLAAALAKTQNELNGQDAQLQTADTRLLSKKSRE
                     FLLDGEGNILIDRYEWFLYQQIPDRLNGQLTLPDITKYRALDADLIDGEHWRKNKYTL
                     LQQSHFTKLAEEPEKLIKQMAMELDTRLYEVGEYLEQEDNRNIILRNPQGKRFWRLPS
                     ASKHHLVNNPFFQQIPTTGIADVLRMVDRDTGFIDCFAHVLGSQSRSRSHEYDLLAIL
                     VGNATNQGIYGMAQISDRTYDQLSTIQANYLRLETLNAANDNINNATAKLPIFRYYNI
                     QEDVIHASADGQKFEARRETFKTRYSSKYFGTQKGVSAMTLIANHAAINARVIGANEH
                     ESHYIFDLLMSNTSDIIPDVLSTDTHGVNHVNFALLDLFGYQFAPRYAQVGKVINDMF
                     DVKEDKEHRIQLCLKKPINTHRIAQHWDTIQRIAVSLKQRKTTQATLVRKLSEYKRNH
                     PLLEALTEYNRLVKANYLLCYIDDASLRNYVQRALNRGEAYHQLRRAVSSVNGDQFRG
                     SSDEEIQLWNECARLVTNAIVYFNSRILSQLLTSFEYQGDTKRIDIVKQASPVAWHNI
                     NLKGTYHFELSEKLPDLEELMRSIEGYLPVSEK"
     gene            3379..>4341
                     /locus_tag="L2X49_004726"
     CDS             3379..>4341
                     /locus_tag="L2X49_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_079376506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISAba125 family transposase"
                     /protein_id="HBN4799689.1"
                     /translation="MEYIKLSYHHLNFEDRTALMLESRKEGFSARKFAELIKRHPSTI
                     YRELKRNSINDVYQARYASDNTFARRRRGHRKLKIDSILWKFIVEAIRCLWSPQQIAK
                     RLKTFPDLDQTMNVSHTTIYSTIRALPKGELKKDLLSCLRHENKKRKANGEPKKDSIL
                     QDIKTIHERPAEVQERKIPGHWEADLIKGKDNKSSIATLIERNTRLCILATLPDAKAE
                     SVRKALTEALKYLPAELRKTLTYDRGREMSEHKILEEDLGIDVYFCDPHSPWQKGTCE
                     NMNGLIRQYLPKGIDLNQADQHYLNQVAMSLNTRPRKALDWLTPL"
BASE COUNT         1281 a         1032 c         1008 g         1020 t
ORIGIN      
        1 gggttctagg gattttccgt ccaaaaacga caaagtgctc tggaggcccc gccgtctgtg
       61 gcctccagag gggtattact tttttcgaga tccctatttg ggctctgttt gagtaagatt
      121 tagtcttttt gggctaaatc catggcattc gaagaacgtg tccaaatctt gtccgaggca
      181 gaacaagatg agttatatgg accccccgct ttcacctccg ccgaccaacg cttctttttc
      241 tcgctgaacg ataaggaact ggcgatcgct aaaagcctcc gtcatcgggg ccagcgttac
      301 atgctggtgg tactgctggg ctatttcaaa gccaagccgg tggtgctgaa tcccggcttt
      361 catcagatca agcaggacct caaatacgtt tatcaaaccg ttctaccagg cccaggctgt
      421 agacccttta atctcacgcc aaaagagaac gaacggattt accaacgtgt tttccagctc
      481 tgcaactacc agcgctggaa tgtcaaagac catggagcgg cgttgagaga ttacttatcc
      541 cagcaagcca gagcctggac agcgcctagg cacctcttcg atgcggccaa tgaatattgt
      601 tcggggcaga agatcgcaat ccctgcctac agcacgctgc aaaagatcat cagtcaggtg
      661 gtgggagacg aacaggaaca catggccgcg caccttgagc gtgcaatgtc acgcggtctt
      721 aagcaagcac tggcggagct cgtgaacggc acaggtccac tgccgttccg acagttgcga
      781 caatcggctc gtaacttcac cggaaccgag ttggagaaag aactgattgt ctatcgccat
      841 atccagcatt ggatgccgga agtggatctg ctgttgagca cgctatcact gtcgcagaaa
      901 aatctgcaac acctggcaga gaaagtcgac tactacggtg ccaaactgaa acgtcaaacc
      961 gtaggcagcc aatggctgta tttattgtgc tatcttcaaa cgcgatggca gcaagcgctg
     1021 gaacgtattg ccgatggctt tgtgcatcat gtcagacaga ccaaacagaa ggcaaaggat
     1081 tatgcgcagg aagcggtgtt taaggattgg caaaaagcag ctaaaaatgt cagcaaggca
     1141 gctgaagtac tgcacctgtt cattgatgac agcattgatc tgcaactacc gtttgcaaca
     1201 gtaagacagc aagcactgag cctgctgacc aaaagggatc tggaatctgt atgtctcttc
     1261 ctgaacgagc agcgacgatc ggtcgatgaa gccatgtggc agtactgcga cgagaaagaa
     1321 agtctgcgga aaggtttgct gcgagagttg ttcctatgtc tgcgcttcga aggctgcgac
     1381 ggcacccagc acttagcggc cgccttggcg aaaacacaaa acgaactcaa cggccaggac
     1441 gctcagttgc aaactgccga caccagactc ctttccaaaa aatcacgtga attcctgctg
     1501 gatggtgaag ggaatatcct gatcgatcgt tatgagtggt tcctctatca acagattcct
     1561 gatcgcctga atggccagct gacgctgcct gatatcacta aataccgggc actcgacgcc
     1621 gacctgatcg acggggaaca ttggcgaaaa aacaaatata cgctgcttca acagagccat
     1681 tttacaaaat tagcggagga gcctgaaaag ctgatcaaac agatggccat ggaattggat
     1741 acccgtttgt acgaagtcgg cgaatatctt gaacaggaag acaaccggaa tatcatcttg
     1801 cgtaatccgc agggtaaacg cttctggcgc ctgccttcgg ccagcaaaca tcatctggtc
     1861 aacaatccct tcttccagca aattcccaca acggggattg cggatgtact gcgcatggtt
     1921 gatcgtgaca ccggtttcat tgactgcttc gctcatgtgc tgggttccca atccagaagc
     1981 cgttcccatg aatatgacct gttggcaatt ctggtcggca atgcaaccaa tcaaggcatt
     2041 tacggcatgg cacagatctc tgatcgtacc tatgatcagc tcagcactat ccaggcgaac
     2101 tatcttcgcc tggaaacatt gaatgctgct aacgacaaca tcaataacgc gacagccaag
     2161 ctacccatct tccggtacta caacatccag gaagatgtga tccacgccag tgccgatggt
     2221 caaaagttcg aagcccggcg cgagaccttc aaaacccgtt attcgtcgaa gtactttggc
     2281 actcaaaaag gtgtttctgc catgaccttg atcgccaatc acgctgcgat caacgccaga
     2341 gtgatcggcg ccaacgaaca tgaatcgcac tacatctttg atttgttgat gagcaatacg
     2401 tcagacatca ttccggatgt gctctcaacc gatacccatg gggtgaacca tgtgaacttc
     2461 gcgttactgg atctgttcgg ataccagttt gccccacgct atgcccaggt tggcaaagtg
     2521 atcaatgaca tgtttgatgt caaggaagac aaagaacacc gaattcagct gtgcttaaaa
     2581 aagccaatca atacccatcg tattgcgcag cactgggata ccatccaacg gattgcagta
     2641 tcacttaagc agcggaaaac aacgcaagcc accttggtga gaaagctctc ggagtacaag
     2701 cgcaatcacc cgctgctgga agccctgacg gaatacaatc gcctggtgaa agcgaattat
     2761 ctactgtgct acatcgatga tgccagttta agaaactatg ttcagcgcgc gctgaaccgg
     2821 ggagaggcct atcaccaact gcgtcgggcc gtgagcagcg tcaatgggga tcagttccgg
     2881 ggcagttcag acgaagaaat ccagctatgg aatgagtgcg ctcgcctggt caccaatgcc
     2941 atcgtttact tcaactccag gatactcagc cagctgttga ccagcttcga ataccaagga
     3001 gataccaaga gaatagatat cgtcaaacag gcatcccctg tggcctggca caacattaac
     3061 ctcaagggga cttaccactt cgaattgagc gaaaaattgc cagatctgga ggagcttatg
     3121 cgctcaatcg agggatattt acccgtcagc gaaaagtaat acccctctgg aggccacgga
     3181 cggcgcggcc tccagagcac tttgtcgttt ttggacggaa aatccctaga accccattat
     3241 ctaaaaatac ttaattgtct tttaacgtcg ctaaatttta aataaataag tgaagagtgt
     3301 tagtggagcc actgatttaa agttggcaga gtaaaacttg aagtgcgaca taaaccacct
     3361 aattaattta aagggtttat ggagtatata aaattgtcat accatcatct taactttgaa
     3421 gatcgtactg cattaatgct tgagtcaaga aaagaaggct tttcagccag aaaatttgct
     3481 gaactcatta aaagacatcc tagtacgatc tatcgtgagc ttaaaagaaa tagcatcaat
     3541 gacgtttatc aagctcgata tgcttctgat aacaccttcg ctagacgtag acgtggtcac
     3601 agaaaactca aaatcgattc aatcctctgg aaatttattg ttgaagcgat ccgttgttta
     3661 tggtctcctc agcaaatagc aaagcgttta aagacatttc ctgatttgga tcaaacaatg
     3721 aatgtaagcc atacaacgat ttattcaacg atacgagcat taccaaaggg tgagttgaaa
     3781 aaagacttat tatcctgtct acgtcatgaa aataaaaagc gaaaagctaa cggtgaacct
     3841 aaaaaagatt ctatattaca ggatattaaa actattcatg agcgcccagc cgaagttcaa
     3901 gaaagaaaaa taccgggtca ttgggaagct gatttaatta aaggtaaaga caataaaagt
     3961 tcgatagcaa cacttattga acgaaataca cggctctgta tcttggcaac attacctgat
     4021 gcaaaggcag aatcagtgcg caaggcttta actgaagctc tgaaatattt acctgcagaa
     4081 ctgcgtaaaa cgttgaccta tgaccgtgga cgtgagatgt cagaacataa aatactcgaa
     4141 gaagatttag gcatagatgt atatttctgt gacccacatt caccctggca aaaaggcaca
     4201 tgcgaaaata tgaatggttt aattaggcaa tatttaccta aagggattga tttaaatcag
     4261 gcagatcagc attatttaaa tcaagttgcc atgtcactga atactcgtcc tagaaaggcg
     4321 ttagattggc ttacaccatt a
//
LOCUS       DAFNBJ010000258         4339 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.259, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000258 DAFNBJ010000000
VERSION     DAFNBJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.259"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="L2X49_004727"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="L2X49_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBN4799690.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(249..324)
                     /locus_tag="L2X49_004728"
     tRNA            complement(249..324)
                     /locus_tag="L2X49_004728"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Thr,seq:ggt)
     gene            complement(331..405)
                     /locus_tag="L2X49_004729"
     tRNA            complement(331..405)
                     /locus_tag="L2X49_004729"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Gly,seq:tcc)
     gene            complement(522..606)
                     /locus_tag="L2X49_004730"
     tRNA            complement(522..606)
                     /locus_tag="L2X49_004730"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Tyr,seq:gta)
     gene            complement(615..690)
                     /locus_tag="L2X49_004731"
     tRNA            complement(615..690)
                     /locus_tag="L2X49_004731"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Thr,seq:tgt)
     gene            1052..2002
                     /gene="coaA"
                     /locus_tag="L2X49_004732"
                     /gene_synonym="panK"
     CDS             1052..2002
                     /gene="coaA"
                     /locus_tag="L2X49_004732"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBN4799691.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2031..2996)
                     /gene="birA"
                     /locus_tag="L2X49_004733"
     CDS             complement(2031..2996)
                     /gene="birA"
                     /locus_tag="L2X49_004733"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBN4799692.1"
                     /translation="MKDYTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2993..4021)
                     /gene="murB"
                     /locus_tag="L2X49_004734"
     CDS             complement(2993..4021)
                     /gene="murB"
                     /locus_tag="L2X49_004734"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBN4799693.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAEALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4156..4271)
                     /gene="rrf"
                     /locus_tag="L2X49_004735"
     rRNA            complement(4156..4271)
                     /gene="rrf"
                     /locus_tag="L2X49_004735"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1110 a         1087 c          969 g         1173 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtagtga ttgcagcggt cagcgttgtt
       61 ttaccatggt caacgtggcc gatagtaccg acgttaacgt gcggttttgt acgttcaaac
      121 ttttctttag acatcgattg tccctctaag acacggataa atcggtgata tcaccacatc
      181 aaccaggcaa catgcccgac ttgttgaatg caataaacag aaggaaaaaa cagggaggag
      241 aaaagaagtg gtgctgatag gcagattcga actgccgacc tcacccttac caagggtgcg
      301 ctctaccaac tgagctatat cagcacatct tggagcgggc agcgggaatc gaacccgcat
      361 catcagcttg gaaggctgag gtaatagcca ttatacgatg cccgcatcct ggaactcggc
      421 tacctgattt tcattctgca ctgaatatcg agagaagctc tctttattcg agccggtaag
      481 cgaacttatc gtctcgggct acgccatcgc gtggccgaaa ttggtggtgg gggaaggatt
      541 cgaaccttcg aagtctgtga cggcagattt acagtctgct ccctttggcc gctcgggaac
      601 cccaccggac ttgatggtgc cgactaccgg aatcgaactg gtgacctact gattacaagt
      661 cagttgctct acctactgag ctaagtcggc atcaagtagc gcgcattcta tggagacatg
      721 cgagttcatg caactaaaaa attgcataat ttgttttatt ggtcacattt tatgcgacac
      781 gatgaagaaa cagccgaacg gagggcagat tagcacactt tttcaacatc attgtgctca
      841 acaatgcgct cctgctaaac cataattctt tttatcagat ggaatatctg tcacattgct
      901 tttcaacgat agcttcctgg cagagatttt ttcttattat tcctccccat ctggtgttac
      961 cctcctgccc attaacccat tcaacagaac tgtgacgcgc catggcaaat atcgctttgc
     1021 cgatagagct atgaccgcca gaaacatgct tatgagtata aaagagcaaa cgttaatgac
     1081 gccttaccta cagtttgacc gcaaccagtg ggcagctctg cgtgattccg tacctatgac
     1141 gttatcggaa gatgagatcg cccgtctcaa aggtattaat gaagatctct cgttagaaga
     1201 agttgccgag atctatttac ctttgtcacg tttgctgaac ttctatataa gctcgaatct
     1261 gcgccgtcag gcagttctgg aacagtttct tggtactaac gggcaacgta ttccttacat
     1321 tatcagtatt gctggcagtg tcgcggtggg gaaaagtaca accgcccgtg tattgcaggc
     1381 gctattaagc cgttggccgg aacatcgtcg tgttgaactg atcactacag atggcttcct
     1441 tcaccctaat caggttctga aagaacgtgg tctgatgaag aagaaaggct tcccggaatc
     1501 gtatgatatg catcgcctgg tgaagtttgt ttccgatctc aaatccggcg tgccaaacgt
     1561 tacagcccct gtttactcgc atcttattta tgatgtgatc ccggatggag ataaaacggt
     1621 tgttcagcct gatattttaa ttcttgaagg gttaaatgtc ttacagagcg ggatggatta
     1681 tccacacgat ccacatcatg tatttgtttc tgattttgtc gatttttcga tatatgttga
     1741 tgcaccggaa gacttacttc agacgtggta tatcaaccgt tttctgaaat tccgcgaagg
     1801 ggcttttacc gacccggatt cctattttca taactacgcg aaattaacta aagaagaagc
     1861 gattaagact gccatgacat tgtggaaaga gatcaactgg ctgaacttaa agcaaaatat
     1921 tctacctact cgtgagcgcg ccagtttaat cctgacgaaa agtgctaatc atgcggtaga
     1981 agaggtcaga ctacgcaaat aattttcagg ggagctaatg ctcccctttc ttatttttct
     2041 gcactacgca gggatatttc accgcccatc cagggtttta ttattccatc ctgctcaagt
     2101 aataaagccc cctgtttgtc tattccgcgt gaaatgccaa atatttcttt atcaccaatg
     2161 ataagtttca ctgggcgatt aataaaatta tccagctttt cccagcgcga cagataaggt
     2221 gccaatcctt cttgttcgaa gagttccaac gcagcacgta attcacgtat tagcatggcc
     2281 gccaacgtat tacgatcgag attgatcccc gcttcctgca gcgtgatcca cccctgatta
     2341 acgacactct cttcaacacg gcgcattgcc atgttgatcc cggctccaat gactatttgc
     2401 gccgcatcgc cagttttgcc agtcagctcc acaagaatgc ctgccagctt gcgatcctgc
     2461 agatagaggt cattaggcca tttgacacga actttatctg ctcccagctt gcgtaatact
     2521 tccgccatca cgataccgat aaccagactt aaaccaatcg ccgccgccgg gccttgttcc
     2581 agacgccaga acatcgacaa atataagttt gcgccaaaag gcgaaaacca tttccgcccc
     2641 cggcgaccac ggccagcctg ctggtattct gcaacacagg catcgcccga tttaagctct
     2701 ccgatacgat caagaaggta ctgattcgtg gagtcaataa ctggtagcac ggctacacta
     2761 ccgccatcca gctgacccaa tatctgttca gcattaagta actggatggg ctcaggcagg
     2821 ctgtatcctt tacccggaac ggtaaagaca tcaacgcccc agtcacgcag tgtctgaatg
     2881 tgtttattaa tagccgcccg gctcattccc agcgtttcac ccaactgctc gccagagtga
     2941 aattcaccgt tcgctaacag ggcaatcaat ttcagtggca cggtgtaatc cttcatgaaa
     3001 ttgtctccac tgcgctcact tcacctgatg caccaataaa gcggacttca ggctcaagcc
     3061 agacattaaa tttttcaccc actttttgtc ttacatggtg tgccagttgc accacatctt
     3121 cgctttttgc attgtcttca ttaatgagaa ctaacgcctg ttgacggtgc accgcagccc
     3181 cacccatttg catccctttt agctggcact gatcaataag ccaacctgct gccagtttta
     3241 ctgaaccatc cgcctggggg taatttggtg ctgttggaaa ttgtgacagt aatgcttcag
     3301 ccgtttcggc agatacaaca gggtttttga agaaactacc ggcattgcca ttcacttttg
     3361 gatcagggag tttggtggtg cgcatatgac acaccgcatt aaatacttgc tgtggcgtta
     3421 ctgttgtagg atccagacga gttaagtcac catacgttag tacaggttgc cactcttttg
     3481 gcagacgcag acctacggct acaatggcga agcgatcctg gtattcatgt ttaaaaatac
     3541 tgtcgcgata gccaaaacgg cactctttgg cagttaagcg cacttgcttg cctgtcgcca
     3601 gttcaacaca atcaacataa gcgcaaactc gctgtaattc tacgccataa gcaccaatat
     3661 tctggatagg tgatgagccg acacaaccag gaattaatgc cagattttcc agaccaggca
     3721 taccttcctg caaagtgtat tttaccaggc gatgccagtt ttctccggct cctacatgta
     3781 aataccacgc atcaggttca tcatgaattt cgataccttt gatccggttg atgatcaccg
     3841 tgccgcgata gtcttccaga aaaagtacat tacttccttc acccagaata agaacgggtt
     3901 gtccttctgc ggttgcatgc tgccaggcat tgagtaattg ttgttcgtct tcggcacata
     3961 caatgtgctg agcattatga tcaatgccaa atgtgttcca gggttttaag gagtggttca
     4021 tagctgcttt cctgatgcaa aaacgaggct agtttaccgt atctgtgggg ggatggcttg
     4081 tagatatgac gacaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     4141 tctgcttaat ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca
     4201 tcggcgctac ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta
     4261 aggccgccag gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc
     4321 aggctgaaaa tcttctctc
//
LOCUS       DAFNBJ010000259         4317 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.260, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000259 DAFNBJ010000000
VERSION     DAFNBJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.260"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..238
                     /gene="nanC"
                     /locus_tag="L2X49_004736"
     CDS             <1..238
                     /gene="nanC"
                     /locus_tag="L2X49_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBN4799694.1"
                     /translation="INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDI
                     TPYIEYDYLDRQGVYNGRDNLSENSYRIGVSFKL"
     gene            258..1364
                     /gene="nanM"
                     /locus_tag="L2X49_004737"
     CDS             258..1364
                     /gene="nanM"
                     /locus_tag="L2X49_004737"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBN4799695.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWKALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1429..2409
                     /locus_tag="L2X49_004738"
     CDS             1429..2409
                     /locus_tag="L2X49_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBN4799696.1"
                     /translation="MNAIISPDYYYILTIAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(2417..3067)
                     /locus_tag="L2X49_004739"
     CDS             complement(2417..3067)
                     /locus_tag="L2X49_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBN4799697.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            complement(4014..4317)
                     /locus_tag="L2X49_004740"
                     /pseudo
     CDS             complement(4014..4317)
                     /locus_tag="L2X49_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
BASE COUNT         1226 a          955 c         1031 g         1105 t
ORIGIN      
        1 tattaatagt gatttcacct tcgcatggca aacgacgcta tacagcaaac agaacgatta
       61 tcgctatgca aaccataaga aatgggcgac ggaaaatgca tttgttctac aataccatat
      121 gacgccggat attacgccat acatagaata tgactacctt gaccgtcagg gtgtttacaa
      181 cggcagagat aatttatcgg aaaacagtta tcgcattggt gtgtcattta aactgtagta
      241 gacaggagac agtcacaatg aataaaacaa taacggcgct tgctatcatg atggcttcat
      301 ttgccgcaaa cgcgtctgta ttaccggaaa ctcctgtgcc atttaaaagt ggtaccggag
      361 caattgataa cgacactgtc tacattggtt taggtagcgc aggtacggca tggtacaagc
      421 tggatacaca ggccaaagat aaaaaatgga aagcgttagc tgcattccct ggtggaccaa
      481 gagatcaagc aacctcggca tttattgatg gcaatctgta tgtgtttggc ggcattggca
      541 aaaacagcga gggcttgact caggtattta atgacgtaca caaatacaac cccaaaacca
      601 atagttgggt taaattgatg tcgcacgcac cgatgggcat ggcgggtcat gtaacttttg
      661 tacacaacgg caaggcttat gttactggcg gtgttaacca gaatatcttc aatggctatt
      721 ttgaagatct caacgaggct ggaaaagatt caaccgctat agataaaatc aacgctcact
      781 attttgacaa aaaagcagaa gattattttt tcaataagtt tctgttgtct tttgatccct
      841 caacacagca atggagttac gctggcgaat cgccctggta cggaacggct ggtgcggcgg
      901 ttgtgaataa aggtgataaa acctggctta ttaatggcga agccaaacca ggattgcgaa
      961 cggatgccgt atttgaactt gatttcaccg gtaataattt aaaatggaat aagcttgctc
     1021 ccgtctcatc accagatggc gtagctggcg gttttgcggg gataagcaat gattctctta
     1081 tatttgccgg aggggccgga ttcaaaggtt cacgagaaaa ttaccagaac ggtaagaact
     1141 atgcgcatga aggcctgaaa aaatcatata gcactgatat tcatctttgg cataacggga
     1201 aatgggataa atcgggtgaa ttatcgcaag gtcgggccta cggagtatca ttgccctgga
     1261 ataatagtct attgattatt ggcggtgaaa ctgcaggcgg caaagcggtg acggattcag
     1321 ttttgatctc tgtgaaggat aataaagtta cagtacaaaa ctaacgcttc agggccccgg
     1381 taaaggggcc ctgctatcaa tttgtcattt caattacgcg aaatttatat gaacgcaata
     1441 atatcgcccg attattacta tattcttacc attgctggtc agtctaatgc catggcgtat
     1501 ggcgaaggac tgccattacc ggacagggaa gatgcgcctc atcccagaat caaacaatta
     1561 gcgagatttg cgcatacgca tcccggaggc ccgccatgtc actttaacga catgattcca
     1621 ctgactcact gcccacacga tgttcaggat atgcagggtt atcaccatcc tctggcaacg
     1681 aatcatcaaa cacagtacgg caccgttggc caggcactgc atatagcacg gaaattactg
     1741 ccctttattc ctgataatgc aggggttctc atcgttccgt gttgccgtgg cggatcggct
     1801 tttaccgcgg gcagcgaagg gacatattca gaacggcacg gagccagcca tgatgcttgt
     1861 cgttggggaa cggatactcc gctataccag gatttagtca gcagaacgcg agccgcactg
     1921 gcaaaaaatc cgcataacaa attcctcggc gtatgctgga tgcaaggcga atttgactta
     1981 atgaccagtg actacgcgtc acaccctcaa cactttaatc atatggttga agcctttcgt
     2041 agggatctaa aacaatacca ttctcagctt aataatatta ctgacgcacc gtggttttgc
     2101 ggcgatacca cctggtactg gaaagaaaat ttccctcatg cgtatgaagt tatttatggc
     2161 aattatcaaa ataatgtttt agccaatatt attttcgtcg acttccagca acaaggtgaa
     2221 agaggactga cgaacgcgcc tgatgaagac ccggacgatt taagcacggg atattacggt
     2281 tcagcgtacc ggtcaccgga gaactggacg acggcactgc gaagcagtca tttcagcacg
     2341 gcagcccgtc gggggattat ttctgacaag tttgtagaag caattttgca gttttggcgc
     2401 gaaaggtgag cgttcattaa tttgatttat tcgcatccat cagcgctttt aactgctccg
     2461 gcgtaaacgg tggtttgcgt cggtgcaaca tcgcatggca attagggtac acaggcagca
     2521 aatcattaac tggatccagc tggtaatcct ctttaatggc tgacagcggc accaggtgat
     2581 gcacatggat aaagccttca gcgatatcac catagatctt caccagatca acaccgcata
     2641 cgttgcactg gcatccatga tgttcgaccg ctttatctcg ggccttttta tcgcgttcat
     2701 agcggtttac tgtcaccttt atcgccgcac cttcaaggta tgtctcctgc gacggcaact
     2761 catccgggaa cagaacgtcg gctatgggag ccgcagcggc tttatccaga tgaatatgaa
     2821 atccctgctc tatgagtagc ttgacacatt tagattttat tccgccggtg aaatccgcag
     2881 gggtaaattg cgtccccgtc atcaatgtcg ctgcgatgcc agcaatcgcc ttcgaggggt
     2941 aacgtcggcc atgatattca atctcataca accgcgcagg ctggaactga tgagtttctc
     3001 ccgcatccca taaatggata gcttcgatga tataccgccg cggaagatga tcagggagtt
     3061 tattcatgga gaatgtcctt atacgttctc agaacaggca gcaaaaaaac aaatgagatc
     3121 aaggtaatat atcttcactt gcaggcttga acagcagaaa atttgcgagc atgatcgcaa
     3181 ttatttcact gccagggata tcaatagctt ccagtaaatc gctacagcag gcaattgcca
     3241 atataaaaat ctggcacaag ggtgaacagc gcgcgccgca taagccattg ttattgctat
     3301 acgtattagc gggatacctg aatggacatc cgcgcctttt cgattattgt agtggaacag
     3361 tctacgagga tgcagttcga agtgagtaag taaaggttgc tggaaacgat caatgccgcc
     3421 atacaatgat tttttaggat caacaatgag taataaaaac tacgaaagtc accgcaaagc
     3481 gattgttagt aaaggtatac cacccgctct attaaatagg ctcaccaatt cggatgttca
     3541 ggtgatcaat accttcttaa cacgagtgag caaactggaa ctatctcaac aagagaaaga
     3601 ctggatcata aaaatcatct ctatggttta gacaatttca gccttgtagc ctttcaaggg
     3661 ttttgtgtgg gtacaaacat tatcgaatcg gggtatagcc gcagctgtag ctgtatccac
     3721 atcagtaagt tgatgtaggt tgacttaggt tgagcgaaaa atagcgaaaa gccttgtgtg
     3781 gcgcggattt cagtcataaa aaaacgtccg ttgatgtcta ttgatgttcc gatggtgcga
     3841 aggtgcgaag gtgcgaaggc cggactcaaa catgtgactt aacctcatga tttaaaattg
     3901 ttaataataa acaatatcat cttataccaa cacagatacc aacacgaaaa atcactggtt
     3961 ttcggcatcg aaccaggaca atcagctcct gattgagata ccgagctctg ttagctggag
     4021 gtaccgtcat gagagttgag cagaataatt tcagggacat gttctccgtt tatctctccg
     4081 gcccgccgca gacgcagcat gtgttttgtg tatccccggc ggcccgggtc gcgcacacgg
     4141 gtctggcagg cgaagaatgg ctgaaagcct tcccgctgca ggctttccag tacggtgatg
     4201 gtggggatgt acgtatagtt ctggcttcgg gacgtgtgtt tatcttcccc gaaaatcccc
     4261 ggcacatagt gcatcagttc ttcgtgtgtc agcggacggt cacggcgtat ctggttc
//
LOCUS       DAFNBJ010000260         4203 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.261, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000260 DAFNBJ010000000
VERSION     DAFNBJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.261"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..453
                     /gene="istB"
                     /locus_tag="L2X49_004741"
     CDS             <1..453
                     /gene="istB"
                     /locus_tag="L2X49_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HBN4799698.1"
                     /translation="PSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGR
                     YKTTLQRGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWD
                     QTFAGDAALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            complement(519..1552)
                     /locus_tag="L2X49_004742"
                     /pseudo
     CDS             complement(519..1552)
                     /locus_tag="L2X49_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
     gene            complement(1864..1939)
                     /locus_tag="L2X49_004743"
     tRNA            complement(1864..1939)
                     /locus_tag="L2X49_004743"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1904..1906),aa:Asn,seq:gtt)
     gene            complement(2040..2837)
                     /gene="mtfA"
                     /locus_tag="L2X49_004744"
     CDS             complement(2040..2837)
                     /gene="mtfA"
                     /locus_tag="L2X49_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBN4799699.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRTCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANNTDSFSATNVH"
     gene            2931..3020
                     /locus_tag="L2X49_004745"
     tRNA            2931..3020
                     /locus_tag="L2X49_004745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2965..2967,aa:Ser,seq:cga)
     gene            complement(3590..4120)
                     /locus_tag="L2X49_004746"
     CDS             complement(3590..4120)
                     /locus_tag="L2X49_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBN4799700.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT         1164 a          955 c          976 g         1108 t
ORIGIN      
        1 ccatcaggtg tggggaaaac ccatctggca atagcgatgg gctatgaagc agtccgtgca
       61 ggtatcaaag ttcgcttcac aacagcagca gatctgttac ttcagttatc tacggcacaa
      121 cgtcagggcc gttataaaac gacgcttcag cgtggagtaa tggccccccg cctgctcatc
      181 attgatgaaa taggctatct gccgttcagt caggaagaag caaaactgtt cttccaggtc
      241 attgctaaac gttacgaaaa gagcgcaatg atcctgacat ccaatctgcc gttcgggcag
      301 tgggatcaaa cgttcgccgg tgatgcagcc ctgacctcag cgatgctgga ccgtatctta
      361 caccactcac atgtcgttca aatcaaagga gaaagctatc gactcagaca gaaacgaaag
      421 gccggggtta tagcagaagc taatcctgag taaaacggtg gatcaatatt gggccgttgg
      481 tggagatata agtggatcac ttttcatccg tcgttgacac tttgccgatc gtctttatcg
      541 aacagggcca cttcatcgcc ataatactgt tcatagacca gtttaccgcc aaggtgcggc
      601 caggcgggta gccagccttc tgcgcgtaca tcccagccat tggccgggcg tgcttcataa
      661 tcgttgtcca gttcaggtgc gctgcgccag ttggtcagtc gcaaatagcc gttactgctt
      721 aattttagat agtcgcgcca gtactccgcg ccaatgccgg cgcgggagtg gtaacggcta
      781 agatcgtggt cgaaaaagaa gttgatgccc gacatccatg tgggagtgaa atgacgccaa
      841 cctaagccgt tgttaatctg cgtacgctcg tcagtacgat ggagagtatg ctgactgaaa
      901 aagagattat caggcgtttc ataccacgga tggagaaaat cgaactggga gttcttcagg
      961 ctaaaatctt catccacgcc cagcgtgatt cttgcggtac cgaagcggct taaccagtct
     1021 gtcattgcgc ctgaagcctg agaagaggcc atccacgcgc catatttgcc gcttgctcgc
     1081 tgttcatatc ctcggcgagc agagacccga tttgctgtga agtactggct atctgttgct
     1141 caaggttgcc actgctatta cccggtggcg gggttaaatt attttcacta acttgtgccg
     1201 ggacatccag ttcatcaccc tggcggacat tatcaaaacc tcgagcaaac gtacgaaact
     1261 ggttgagttt gcgtaactca gccaccgaaa taccgaaacg ttcggcaacg ctttgggccg
     1321 attccagcgc tccaagggtg tagggcaccg tattggcatt tgcaatggca atttgtgcag
     1381 gaactggttg ttgggttgcg gcgtttacca caccttgtgc tgccgcagcc atagggaaca
     1441 caagttgagt taccaggcag atacccgcag ttaagcggca tagtttgata cccatcccgc
     1501 ataagatttg tcggtcattt atttcttctc cacttctctt cttcgtagcc atgcttacct
     1561 tccctgaacg agctaacata aatgcgccct ttctagcaca gcgaaaaaat gtaaatcctg
     1621 atagcaatat gattgaaaat taataacttt tcgttaggca gttttgggtg tgagttgcaa
     1681 gaggggagac tactgaatta ctcaagtttt ataatcgagg ggaaaatggt gatgacgcca
     1741 tagcaaaacg ccctcaacca taaagttcga gggcgcttaa gatgttaaaa acccgctatc
     1801 cgttaaaaaa caatgttcaa ctaaggttag tgacattgca ctaagaagcg aatttttaga
     1861 atttggctcc tctgactgga ctcgaaccag tgacatacgg attaacagtc cgccgttcta
     1921 ccgactgaac tacagaggaa tcgtgtgaac ggggcgcata ttacttagcg gtaccttgtc
     1981 tgtcaacact aaatttcata agtcatttca attggttaat taatctgcaa agttgttaat
     2041 taatgaacat tcgtcgccga aaacgagtct gtattattag cgtgatgcag tctctgcaaa
     2101 ggatcttgtt gataaaattg gcagaaacgt tgccacaatg aagggaaacg aggagcaaaa
     2161 agttctgggg cgctaaagaa atattcagaa agtacggcaa aacattcagc aggatcactg
     2221 gcagcataag catcaatgct cgccgcattc tcgccaacca attcgatttc ttcctgaatg
     2281 ttgttcattg cagcatgaag atcgtgttcc cagccagcaa cctcacgcaa cggaataaag
     2341 ggaactccgc tggcgcgatc gccgttacgg gtgtccagct tatgagcgac ttcatgaata
     2401 atcaggttaa aaccagaagc atcaaaagaa tcttgtatat ccaaccagtt caaaacgata
     2461 ggcccttgct gccagctctg acctgactga acaatacgtt ggttatgcac cagaccgata
     2521 tcgtcttccc attcatcatc gaccacaaat ggcgcaggat aaattaagac ttcatgaaaa
     2581 ccatccagcc attccagtcc taattccaga acgggtaggc aaaatagaag tgctatccgg
     2641 caggttctta atgaatccag ttcaaagccc tgtaaaggaa caagccgctt ttgctgtaaa
     2701 aaacgttcgg caagagtgac taatttgctt tgttcctgtt ccgtcagaca cgttaaaagg
     2761 gggatcgata gtgcttcctg ccagggaagg gcagtttgat gtgctgattc ttgtactttc
     2821 cagggccact taatcatcgt tttgctcgca aactcgtcac ttgaacaaaa ttgcacggac
     2881 agggactgtt aaaatgccaa atttcctggc atcatggcaa ccatctgaac ggagagatgc
     2941 cggagcggct gaacggaccg gtctcgaaaa ccggagtagg ggcaactcta ccgggggttc
     3001 aaatccccct ctctccgcca ctttatcaat gacttatctc ccgacttccc gccttgcttt
     3061 tcctaaacag aacaatcgta gaatattctt gaagggttag atcgtcactg ttttctgttc
     3121 gatactgtga cattcagcac ttgattcgct atggatctga caggaagctt tcgagcgaaa
     3181 atgtgcagtt attcagtcgt tttcttatcg ctcaccatta ttcttttaga cattcatcct
     3241 atgaaactgc cgcaaagttg gtggtgggaa ctgaagttgc gtagagaagg ggtcaataat
     3301 tgagtcaaaa aaggaacgcg gccgtcagat taaagccgcg ggacaaagtc cataatttcc
     3361 aagagttgtt gaatttcgca gcttttttga aagcctttgt atcatactaa caagtaaaaa
     3421 aggtttaaat accaaaatta acctgtattt ttgataagtc ttaccattgt acgatttaca
     3481 cagtttctgc tcctccttgt tgatagtgga aagggtgcat tgtgcacttg cgtaaacatt
     3541 tatgacgcgt tgatgtccat ggttcacatt tccttgtaag gggatcgact tatttgcggc
     3601 gaggcatcat tcgtaacaga gtattatctt tctgaatgta gtgatgaaag agtgctgcgc
     3661 cagcgtgagc tgcgattaaa aagtagccta tatttgccca ggtttcgtga atatttttta
     3721 tcagtgcttt aatttcgctg tttggggtaa caaagggaga cacattgaaa ccaaggaaac
     3781 tccacgattt tccactgtaa gccatcaaag caatccccag cagaggaagt gcaaggaagg
     3841 ttatataaag catgatgtgc attagcgaag ccgctttcat ttgccaggca ggtggcggtg
     3901 gcacaataga aggatcacta taacggtgtt ttataatcag gcgtgaaaac attaacaccc
     3961 aaacgaatat acccgcattg taatgtgttt ctcgcatcag cagataagta ctactacctt
     4021 taggaaacca gccacggagt tccatcgcgg cataggttat tgcaacgaaa agcagcgtta
     4081 tccaatgtaa ataaatttga gtttttgaga atcggttcat ttttgtagct cctgtgattt
     4141 tcacagtcaa gctatcccac gaaccttaaa aattccttat acagatgtgg ttttaataat
     4201 ttg
//
LOCUS       DAFNBJ010000261         4073 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.262, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000261 DAFNBJ010000000
VERSION     DAFNBJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.262"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            75..776
                     /locus_tag="L2X49_004747"
     CDS             75..776
                     /locus_tag="L2X49_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBN4799701.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPD
                     EQKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRA
                     QYFKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTG
                     DRTIAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1034..3181)
                     /locus_tag="L2X49_004748"
     CDS             complement(1034..3181)
                     /locus_tag="L2X49_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBN4799702.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEAL
                     PKDSNSAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGL
                     AALRAQLLKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQR
                     VYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQG
                     RLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVI
                     SALTDKGLRYAGYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLF
                     RSLGFNRTWEEWQREEDARTHTTERRHDRGWSQ"
     gene            3360..3614
                     /locus_tag="L2X49_004749"
     CDS             3360..3614
                     /locus_tag="L2X49_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HBN4799703.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
BASE COUNT          962 a         1031 c         1067 g         1013 t
ORIGIN      
        1 aaaaaaaaaa cttccaccca tatgataagt ggcgtacagt aaaaaaacgc caccaatagc
       61 aacgagcaag cgaaatgtca gatctgatag cttacaaatc aaacgccctt atcgaggcaa
      121 gctacaagct cactttgcag gaacagcgct tcctgctggt ctgtatcagc cgcctaaaat
      181 ccggagaaga ttccccttcg ccggatgaac aaaaaaccat gaccatcacg gctgcagagt
      241 ttcatgatgc ctttcctgac atgggacggc agcacgctga agctaggctc aaggaagcga
      301 tagatcgcct ctgggacagg tcagtaatca tcaagaacga ggaaaaacga gaagagttcc
      361 gctgggtgca gtaccgggcg cagtatttca aaggtgaggc gaaagtagat atcaccttct
      421 ccgatgccat catgccgtat ctgacacagc tcaaggggca gtttacgcgg gtagtcgtga
      481 agaacgtctc cagcctttcc agcacctaca gcatccgcat ttacgagctg ctccagcagt
      541 tccgcagcac cggtgacaga acgatagcta tcgatgattt ccgaaccatg cttgccgttg
      601 aagataaata tcaacaattc aaagacttga ataagatgat aatcaagcca gccatcgccg
      661 aactgaacga aaaaagcgat ctggttgtaa ctgttgagac gatcaaaaag gggcgcacgg
      721 ttgtagccct gcattttcgc ttcaaggaag acaaacagat caagatgacg atctaaaatc
      781 gattttaagg cacctaaaac gctccctgct gcgttttttt tgtgttagta gtgcgaaggt
      841 actattgaag tgaaaaattg cgtcaaaaac gctctgatgc agcgaaaaaa gaccccccaa
      901 tttttggggg cggaaaaaca aaactatgct tgcgttatgt gagtgtgctt cttgcattca
      961 tccctgtcga tgaacatcga agaacatcga cgaacataac acgatgttaa acaaaacaaa
     1021 atattgttca cattcactgt gaccaaccgc gatcatgtct cctctcggtg gtgtgtgttc
     1081 gtgcatcttc ctcgcgctgc cattcctccc aggtacgatt aaaacccagc gagcggaaca
     1141 gatgatccgg ctgtttttct ggtgtgttga cggcagcaaa aagcggtgcc tcacgggttt
     1201 tcagatgaaa ctgcatcagt tctggtgcag gtgatatgtg gctgtctgca tcgtgattta
     1261 cggcaacacc ataccctgca taccgtaacc ccttatcggt cagcgcactg atgaccagat
     1321 gcctgttatc ggctgaggga acggcaaacg cgggatacag cgtaccatta agccggataa
     1381 caccgcccag agagtcatcc acgcccatga ccagcaaaag ctgaagctgg cgggtttccc
     1441 ggtcgacaga actcatgacc ggcatcagcg tcggtggcag acgtccctgc ataaaatcca
     1501 ccttccttgc cgctggtgat ggcatggctg gctggcggtc ttctccaaga cagaaagact
     1561 ccggccatgt cttcggctgg cggagattgc gggtatgttg cggccatgtc tggcaaacct
     1621 gcgtcagcag ttgctcaaag gttgcagagg aataaacacg ttgcagtggc ggacgttggc
     1681 cgatttcgct tcggtcacag atataaccac tcctgtcacg cattaaatca acactctcaa
     1741 agattctggt gcggtatttc tccagctcgt tcccccagag cgtatattct ccgttccggt
     1801 tgtgaagcag aaggtaacag gtcatttccc cgttaccagg catgggagcc tcgccatacc
     1861 ccgtaacgac tgctctcagc acccctgaat ccataacggc attgtcattt ttcctgagct
     1921 gctccgcgcg actacgttca gtatcttcct gttccttccg gcgcaattcc tcttcacgtt
     1981 taagcaactg ggctctgaga gctgcaaggc cggattcccc ccactgctgc cactgttttt
     2041 caaatgccat ctgactcgcg ggaatgtcct gtttctccac cttccggtaa tgctgtcgta
     2101 atttttccat ttgctggctc agagactgga tttttccctg ctcactacgg tattccggtg
     2161 ccgcccattg catcagggga atacgggaaa aaccggacga aaacggcgat gattttttca
     2221 ggctggcagc atggctcatc gcttcctttc gttcggtgtt cagctcttcc cagcgtctgc
     2281 gcgtcgcctg catatctgcc atcatcgaca tggcattact ggtgagtttc catgccgagt
     2341 tactgtcctt cggcaacgct tcgataatgt cacgtagtgt gtacgaagtt gcgttgtcag
     2401 gaagcccgat attcagaagg tcagccacac ggcgggcagg ctctgtcagc cctccgttcc
     2461 tgagttcggt gcgggaaatc tgtatttcca ggttaagcct gttaatcaga cgattctgtt
     2521 cgccgcgttc tgtttcgata ccgcgtgttt ccatcgcaca ggcagacttt ccgaggtgaa
     2581 taccaggcgt tttctcaagc ccctgtcgct cgtatgaacg atggtctatc tcttcctgat
     2641 aaccggcgtt cgccagtgcc ctgtttgcat ggtcagccca tgatgcccgc cacgtctcgg
     2701 catgtgtccg gtcgttccag tccctgacct ttccgctgaa cccagcaggt ccgatagccc
     2761 tcgtggtcag catgatgtgc gcatggggat ttgttttatc catgtgatgg aacgccacat
     2821 cagcaatcat gccctgactg acaaagtttt ctctgacaaa ggcgagaacg gtttcccgtg
     2881 cggcatcctg cggcagttcg tgggggatgg cgagttcaat ttcccgcgca agttgtgagt
     2941 tcttccggcg ttcggccttc tcaacggcat tccagagttc ggcacggttc gtacaccagg
     3001 caggggcggt ggcgggggtc agaatcacag cctcagccac accgcttttg cgggtgtagt
     3061 cgtgagtcag tccggtacgc tcatcaagca tcagtgaacc ggaacgatag gcagcggaag
     3121 ctacggcgct gtagccgctg cttctggaga taattttcat gctgagatga taaatcgcca
     3181 tttctgccgt aatgcctttt tgtagttttc tttcagcccc gcagggcgca cacactcgaa
     3241 acggagtgga gattgtataa gtgcgcactt cgtgatttgc cgtgcattat gccagctcag
     3301 tttttaaact accagtgacg cgctaaaatg cgatttttca tcgcgcacgg agttacatca
     3361 tgagcagaac gctcgaacag aagattgctg atgctgaggc gagattacag cgcctgaagg
     3421 cgaagagccg gagcctggac acagcgcaga aggttgttgt gggtgcagca ttgctggcaa
     3481 aggtcagaaa gccggaggag gtgcagttgc gtgcctggtt actccagttc ctgaaggcag
     3541 aggtgacacg acaggctgat gtgacgcgca tactgccgct gattaacgag ctggaggcgc
     3601 tgccagaaca gtgattgtat cgtccgctcg ccgcagcccc agcgaccgag cgttagcgag
     3661 cgagtgggcg aggaagcgga agtgaatcag tcgccaggtt gagcacatac gcacggtttt
     3721 tcggcaggtg atatgcgctg aacccgcatg aatactgggt ttccacaggg ttggaggggg
     3781 tgtattcgaa acttgcaatt ctgctttatt gcaacgcatt gtttttattg gttttttgtt
     3841 ttcagcaccc caagttatcc acaggctcgc cgttaagttt ttaagatctt atttttagat
     3901 cttttttttt aaaacctttt taggtcttgt aacaccatga ttttaaagat gttctggcga
     3961 gataagtgga gcttttgggg gtgaaaggtg gagcttttgg gggtgaaagg tggagctttt
     4021 gggggtgaaa ggtggagctt ttgggggtgt aatccacaga aaaagggggt tgc
//
LOCUS       DAFNBJ010000262         4042 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.263, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000262 DAFNBJ010000000
VERSION     DAFNBJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.263"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            11..1459
                     /locus_tag="L2X49_004750"
     CDS             11..1459
                     /locus_tag="L2X49_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBN4799704.1"
                     /translation="MLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADILATEH
                     FSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQMDGLE
                     TVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVE
                     ALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFPSTMV
                     DRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSD
                     VLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAPTLKV
                     QGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLA
                     LGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDL
                     PDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1495..1698)
                     /gene="ydfZ"
                     /locus_tag="L2X49_004751"
     CDS             complement(1495..1698)
                     /gene="ydfZ"
                     /locus_tag="L2X49_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBN4799705.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1876..2562)
                     /gene="ydfH"
                     /locus_tag="L2X49_004752"
     CDS             complement(1876..2562)
                     /gene="ydfH"
                     /locus_tag="L2X49_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBN4799706.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2651..3397)
                     /gene="ydfG"
                     /locus_tag="L2X49_004753"
     CDS             complement(2651..3397)
                     /gene="ydfG"
                     /locus_tag="L2X49_004753"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBN4799707.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3534..>4042
                     /locus_tag="L2X49_004754"
     CDS             3534..>4042
                     /locus_tag="L2X49_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidyl carboxypeptidase II"
                     /protein_id="HBN4799708.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLT"
BASE COUNT         1012 a          973 c         1024 g         1033 t
ORIGIN      
        1 gggaaataat ttgttatcag caaaagcgac actccctgtt tatgatcgta ataacctggc
       61 cccaagaatt attcatttag gctttggtgc atttcaccgt gcgcatcagg gcgtgtatgc
      121 cgatattctt gctacagaac atttcagtga ctggggatat tatgaggtca acttaatcgg
      181 cggcgaacag caaattgccg atttacagca gcaagataat ctttataccg ttgcggaaat
      241 gtcggccgat gcgtggacgg ctcgcgtcgt tggcgtcgtt aaaaaagcct tgcacgtaca
      301 gatggatggc ttagaaaccg tgttggctgc gatgtgtgaa ccgcaaatcg cgattgtctc
      361 tctgacaatc accgaaaaag ggtatttcca ctctccggcg accggacagt taatgctcga
      421 tcacccgatg gtcgctgccg acgtgcaaaa tccccaccag ccgaaaacag caacaggggt
      481 gattgttgag gcgctggccc gccgtaaagc ggcaggactt cccgcattta ccgtcatgtc
      541 atgtgacaac atgccagaaa acggtcatgt tatgcgtgac gttgtcactt catacgcgaa
      601 agctgttgat gtaaaactgg cacaatggat cgaagataac gtgactttcc catcaacaat
      661 ggtggaccgt attgtgcccg cagtgacaga ggatacgctg gcgaaaatcg aacaacttac
      721 cggtgtgggc gatcctgcgg gcgttgcctg tgaacctttc cgccagtggg taatagaaga
      781 taactttgtt gccggacgtc cagaatggga aaaagcggga gccgaactgg ttagtgatgt
      841 gctgccttat gaagagatga agttgcgcat gctcaacggc agtcattcat tcctggcgta
      901 tttgggttat cttgctggat atcagcacat taatgactgc atggaagatg aacattatcg
      961 ttatgcggcg tatggcttga tgttgcagga acaagcgccg acgctgaaag tgcagggcgt
     1021 tgatttgcaa gattacgcta accgattaat tgcacgctat agcaacccgg cgttacgtca
     1081 tcgaacctgg cagattgcga tggatggtag ccagaaattg ccacagcgga tgttggattc
     1141 tgttcgctgg catctggcgc atgacagcaa gttcgatctg ctggcgctgg gcgtcgcggg
     1201 ttggatgcgt tatgtcggtg gtgttgatga acagggaaat ccgatagaaa tcagtgaccc
     1261 actgttacct gttattcaga aggctgtaca aagtagtgcc gaagggaaag cgcgcgtcca
     1321 gtcattgctg gcgattaagg caatctttgg tgatgatttg ccagacaata gcttgtttac
     1381 tgcaaaagtg acggaagcgt acttgtcttt attagcgcat ggcgcgaaag cgaccgtggc
     1441 gaaatattcc gtgaagtaaa aaaagcaatg ccgccatcgg cggcattcac acacttagtt
     1501 catgccgaga cgaatcaggt ccagaggtgc cagtttctct tcacaacctt caacaactac
     1561 ggtttttccg cggcggattt gctcagcggt cagaccttca gtatcaatcg acaggatctt
     1621 gccagtgtga ccggtgccgc taaccataac acggctgcca gtggtgattg cgttacggtt
     1681 acgatcgtag gtcgtcatag gtgttttctc ctttctgatt tacagtcgaa gcccacgtaa
     1741 cgggcgcaat ataaatactc ctgtcgagtg aaatattttt tgtttttgat caagctcaca
     1801 tcttttttga tgcatgcatg agccgtaagg cggggggaaa agcctctcat cccggatggg
     1861 atgagagtaa atgaattact cttcgctaaa ccagtcgctg ttttcctggc ggatctggcg
     1921 cacggattcg ctgatttcct gcaaatgttg cgtcattgca cgttctaccg catcgccatc
     1981 acgtttttgc agggcagaga aaatatcaag atgctggcgt aacagcattt ctggtggaga
     2041 aacatggtcg aaactcatat agcgcacgcg atcaacggtc gctttcaggt tctcaatggt
     2101 atcccacgcc agttgacagt cggcaatctg cgtcaggagt tgatggaagt tgtcatcaag
     2161 ttcaaaaaaa tcatccagtt gcttgcgctc aatggcaatg cgttgctggt gaagattttg
     2221 ttccagttgg tagcactggc tttcggtaat catgctcgcc gcccgacgcg ccaccgcgca
     2281 ctcaatggcc tgacggataa aactgccgtt tcgcacctgg gccatggaaa ttttgttgac
     2341 gtagctgcca cgctgcggac gaatttgaat caggccgttt tccgccagtt taataaaggc
     2401 ttcacgaacc ggctggcgtg acacattgaa acgaacagaa acttcttttt ccgacaacgg
     2461 tgtgcctggt gcaatcaggc aatggacaat gtcgcgacga agaatacgat aaatctgctg
     2521 attgacaggc tgtgtgggat taagttgcgt ttcgacggtc attcgttctt gcttgtgagt
     2581 gagttaactg catgagtcta ccactttttt gtgacaagca ataacccggc agttacgccg
     2641 ggtataaaaa ttactgacgg tggacattca gtccggcata gctttgggta accggcatca
     2701 tttccagggt attgatattg acgtgagcag gcagcgttga cacccaccag acggcttcgc
     2761 tgacatcttc tggcgtcaat gcaacggtat tttgataggt tttttccgct ttaccgtcat
     2821 cgcctttaaa gcggacattg gaaaactcgg tgccacccac cagacccggt tcgatgtcgg
     2881 tgacgcgcac cgccgtacca tgcagatccg tacgcagatt caggctaaac tgacgaacaa
     2941 acgctttcgt cgcaccgtaa acgttaccac cggcatacgg ccagctacct gccgttgagc
     3001 caatgttaat aatatgacca tgattacgtt caaccatacc cggtaagacg gcgcgcgtca
     3061 tatataccag gcctttgttg ttggtatcaa tcatcgtttc ccagtcttca acgctggctt
     3121 tatgcgcagg ctccatgccc aacgccaagc cggcattatt taccaggata tcaatattgc
     3181 accactcggc aggaagcgat gccagcatct cttcaatagc ggcgcggttg cgaacgtcca
     3241 gttgggcgat atacagatta tctcccagtt cgtcttttaa ctcctgcaac cgctcctggc
     3301 gacggccagt ggcgataact ttatgccctt gttgaataaa acgacgagta atgcattcac
     3361 caaaacctgc cgttgctcca gttactaaaa cgatcatctc actgttcctc aacgcttttg
     3421 tgtgtcaata acatagcacg tgatgcttaa gagcgggtaa tactggtttg caagattgca
     3481 ctccgttatc agtaagccta cgctatggaa cacctcgcgt tcaggagaag aaaatgacaa
     3541 caatgaatcc tttccttgtg caaagcacac tgccgtatct ggctcctcat tttgatcaaa
     3601 ttgccaatca tcactatcgc ccggcattcg atgagggaat gcagcaaaag cgggcagaaa
     3661 ttgctgccat cgcgcttaac ccgcaaacgc ctgatttcaa caatactatt ctggcactgg
     3721 aacaaagcgg agaattactt acccgcgtta ccagcgtctt ttttgcgatg actgcggcgc
     3781 ataccaatga tgaattacag cgtcttgatg aacagttttc cgctgaactg gcggaactgg
     3841 ctaatgatat ctatctgaac ggtgaattat tcgcgcgggt agatgctgtc tggcagcgcc
     3901 gtgaatccct ggggcttgat agtgaatcca tccgcctggt ggaggttatt catcaacgtt
     3961 ttgtccttgc cggagccaaa cttgcgcaag ctgataaagc aaaattaaaa gtactgaata
     4021 cagaagctgc gaccctgacc ag
//
LOCUS       DAFNBJ010000263         3998 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.264, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000263 DAFNBJ010000000
VERSION     DAFNBJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.264"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..722)
                     /locus_tag="L2X49_004755"
     CDS             complement(<1..722)
                     /locus_tag="L2X49_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBN4799709.1"
                     /translation="MRLLLSLDMKILRSSYLKVRGFLIKGCIVVLNYNLCGVMICLHR
                     KGYMRLIVKTVTGLTKVRHRNEVGVTLASLSLSAKRVLFLALCQIDTKEMLDDDILEV
                     DADFFSKATSLDKYASYAALKEGAKVLSSTTLVLNRDDLKNLADELGILSSKNKIPDR
                     LDLNLTEFCAYYDHLATVRIKFTNTAKRYFSKLIGSENRYTTQVLKSVVLLNSVNSTN
                     LYQVIRKYYSQNSSKKSFDISV"
     gene            1333..1485
                     /locus_tag="L2X49_004756"
                     /pseudo
     CDS             1333..1485
                     /locus_tag="L2X49_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(1663..1821)
                     /locus_tag="L2X49_004757"
     CDS             complement(1663..1821)
                     /locus_tag="L2X49_004757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799710.1"
                     /translation="MRAITPLTPARPISDYQTMFSLSQLARELANGTPGQCETLARGA
                     VSARYEMR"
     gene            2075..2590
                     /locus_tag="L2X49_004758"
     CDS             2075..2590
                     /locus_tag="L2X49_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HBN4799711.1"
                     /translation="MNIQEALNVFGLSGDLTEKDIKAAYKKAALKYHPDRNPLGAELM
                     KAVNAAFDFLMANIDKINQFQSTDENARYNYGEDLEKVLNTLSGLTGIVYEVIGNWVW
                     ISGETKEHKDILKEMGCKWASKKKQWFYRPEEHKSRWNRKEHSIEEIREMYGTAGKRK
                     ASGWTRVEASA"
     gene            2649..2885
                     /locus_tag="L2X49_004759"
     CDS             2649..2885
                     /locus_tag="L2X49_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799712.1"
                     /translation="MKNKTITEAELINIFESYGAYICPDEIEVTAKECNENGSVLHRG
                     LNAEGWAHLFAKEEAYQQECEAQEAASDDGHFDE"
     gene            2945..3496
                     /locus_tag="L2X49_004760"
     CDS             2945..3496
                     /locus_tag="L2X49_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799713.1"
                     /translation="MKEQKNFFERYKPVFEIVCRILGNGWRVNLLDDCQYRIKLTSPD
                     FKNYSIHIRMEKGRLVIIGSVDSRSWRSPYHTCTVSPERNPVEIAADIEKKILSDALD
                     NVDMAREYEQQLQQKREKKLILKGMLSRLVHLESWHGTLTGFKVENGLDGNVSERGDG
                     YEMVIRGLSVDQLIKVAGFIKQL"
     gene            3562..3774
                     /locus_tag="L2X49_004761"
     CDS             3562..3774
                     /locus_tag="L2X49_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799714.1"
                     /translation="MSEDATRLFIELEKLMKVRSKEADKKTTKKIVFQKSFYYEEFSN
                     VKDEIDDPILLKFYTDTIVKVQNHEF"
     gene            3764..>3998
                     /locus_tag="L2X49_004762"
     CDS             3764..>3998
                     /locus_tag="L2X49_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799715.1"
                     /translation="MSFKPTPEEVKQARIKAGFTQQEAAERFGFTLSAWQAKETSGKT
                     SRGLAAGTYELLLLLADEHPDYQLVKREKNQDKV"
BASE COUNT         1258 a          820 c          858 g         1062 t
ORIGIN      
        1 tccacggata tatcaaaaga tttttttgaa ctattctgac tgtaatattt cctaatgact
       61 tgataaaggt ttgttgagtt tacactgttc aacaacacaa ctgatttcaa tacctgagtg
      121 gtatatctat tttcagaacc aatcaactta gaaaaatatc gcttagctgt atttgtgaat
      181 tttatccgta cagttgcaag atgatcgtaa taggcacaga attctgttaa gtttaaatcc
      241 aagcgatcag gtattttatt cttagatgat agtatcccaa gttcatcagc caaatttttt
      301 aaatcatctc tgtttaaaac taacgtagtt gaagaaagaa ctttagctcc ctctttcaga
      361 gctgcataag aggcatattt atctaaagaa gtagcttttg aaaaaaagtc agcatcaacc
      421 tccaaaatat catcatctaa catttccttt gtatcaatct ggcaaagagc cagaaaaagc
      481 actctttttg ctgaaaggga aagggatgca agagttaccc caacttcatt tctatgtcta
      541 actttcgtta atcccgttac tgtcttcacg ataagcctca tataaccttt cctatgtagg
      601 caaatcataa ccccacaaag gttgtaattc aagacaacaa tacaaccttt aattaaaaaa
      661 cctctcacct ttagatatga agatctgagg atcttcatat ctaaagatag caacaaccgc
      721 atgtttcact tgatgttcag tcaaactgtg gaaaccgtta aaaatcttct caccttacgt
      781 gaaaaatcct ctcatcttta gtgaaaaatc taactacctt aacttaaaaa tcttctcacc
      841 ttacgtgaaa aatcctctca ccttacgtga aaaatcctct cacctttagc aatttttttc
      901 cataattttc aggtaattga agcgagctaa gtttttaagt ttttaagatc aagaagaaaa
      961 tataaggctt ttaatgcaca gcgtctgtgg ataactcagc ataaatccag aggatagatc
     1021 cgtctggcct ccctgagcac tgcaccacca aaaattgagc aaatattaac cttagcgcgt
     1081 aagtgctcta ttgaaacccc gtggaaattc cgcttcctcg ctcactcgcg ggcttcgccc
     1141 tgtcgttcgg ctgcggcgag cggtgtaatc tctctaaaag atccagacgg gatccttttc
     1201 cgatccaatt cttaaatgct ctcaaaagcc ctcagaggcg ttttaaccgc tttaacccgg
     1261 tcatggttca acggagcggg taaaatgcgc ttagaacgtc atacagctcg tttttaacct
     1321 gatccctaat cagggggtat aacagaggcg gccaaaaaat ccggccttgc tcgtcagagt
     1381 atctaccgcg cattatcccc gaacggaaac ccgaccatca caacacttgc acagcttgca
     1441 gcagtagccg ggttacagtt cactctcagt aagcccaacc actaaccttt cagacaagcc
     1501 cctcataccg gggggattat ttcttatgcc agcaaaattt cacgaccggc gaggatggcc
     1561 aagtgttttt tgctggcaat tttcgtgcag cagctggcgc ccggattcat tttttgctgt
     1621 caggcatcaa tcggtttcag caacccgccc cgtaattcgc tgctaacgca tctcataacg
     1681 cgcagataca gcgccccgcg ccagtgtttc acactgaccc ggcgtaccgt tcgcaagctc
     1741 acgcgctaac tgcgacaggc taaacatcgt ctgatagtca gaaattggcc tggcaggggt
     1801 gaggggggtt atcgctctca ttcgggcttg ttggttcgcc agtcctggcg aacgtctccg
     1861 gcgcgatgtt acgacagcgc attatgtaac tacagttcgc gcctctttcg gaaatccctt
     1921 catgcgcggt aaagcaatat acatctttat gtaatcttta caatgtaaag cattaaaaat
     1981 gattccataa ccatcatttg atggtataat gaatttgttg gttgggaatg gttccctgcc
     2041 agattccaga agcccacacg ggcgggagcg aaaaatgaat attcaggaag cattaaacgt
     2101 ttttggttta tccggtgatc tgactgaaaa ggacatcaag gcggcataca aaaaagcggc
     2161 tttaaaatat catccagacc gcaatccatt gggtgccgag ctgatgaaag cagtaaatgc
     2221 tgcgtttgat tttctcatgg ctaacattga taaaatcaat cagtttcaaa gcactgatga
     2281 aaacgcacgt tacaactacg gtgaagatct ggaaaaagtt ctgaatacgc tttccggcct
     2341 tacagggatt gtctatgaag ttattggtaa ttgggtctgg attagcggag aaactaaaga
     2401 acacaaagac attttaaagg aaatgggctg taagtgggca tctaagaaaa aacaatggtt
     2461 ttaccgtcct gaagaacaca aaagccgctg gaaccgtaaa gaacacagca ttgaagaaat
     2521 tcgtgaaatg tatggtacag cgggtaaacg taaggcgtca ggctggacac gtgtagaagc
     2581 aagcgcataa ccggaatggg ggcgaaagcc ccctagaaca tggcaaagat tgattcggag
     2641 acacgttgat gaaaaacaag accataacgg aagcggagct gataaacatt tttgaaagtt
     2701 acggagccta catttgcccg gatgagatcg aagttacagc caaagaatgc aatgaaaatg
     2761 gttcagtctt gcaccgtggc ttaaacgcag aaggatgggc gcatcttttt gcgaaggaag
     2821 aagcatatca gcaggaatgc gaagcccagg aagctgcaag tgatgatgga cactttgatg
     2881 aatagtctta aaactgccgt aattcgattt acggcttttt tattgagttc aaaaggctga
     2941 atcgatgaaa gaacagaaaa atttctttga acggtataaa ccggtgtttg aaatcgtttg
     3001 tcgaatcctg gggaacggct ggcgtgtaaa tctgctcgat gattgccagt acaggattaa
     3061 gctaacctca cctgatttta aaaattactc tatacatatt cgaatggaga aaggaaggtt
     3121 agttatcatt ggaagcgtag atagtcgcag ctggagaagt ccttaccata cttgcacagt
     3181 atcgccagag cgtaaccctg tagaaatcgc cgccgatatt gaaaagaaaa tcctgtcgga
     3241 tgctttagat aatgtagata tggcgcgaga gtatgaacaa cagttacagc aaaaacgcga
     3301 gaaaaaactg atcctgaaag gaatgctatc tcgtttagtg catcttgaaa gctggcatgg
     3361 gacattgacc gggtttaaag ttgagaatgg gttagatggt aacgtatcag aacggggcga
     3421 cggatatgaa atggtaattc gtggcctgag tgtagatcag ctcataaagg tagcggggtt
     3481 tattaaacag ctatgaggca aaaaatgaat gaagtcctga gcgacaggaa aaacaaaggt
     3541 aatgtaacat acaggattgt tgtgtctgaa gatgcaacac gtttgtttat tgaacttgaa
     3601 aaactgatga aggtacgctc taaagaagcg gataaaaaaa caactaaaaa gattgttttc
     3661 caaaaatcat tttattacga agaattctcg aacgttaaag atgaaattga tgatccaatt
     3721 ttattgaaat tctatacaga tactattgtg aaggtacaaa atcatgagtt ttaagccaac
     3781 acctgaagag gtgaaacaag cacgtattaa agctggcttt acgcagcagg aagccgctga
     3841 acggtttggt tttacgttat cagcctggca agccaaagaa acatccggta aaacgtctcg
     3901 tggtttagcc gctggtacgt atgaattact tttgctcctg gctgatgaac atccagatta
     3961 ccagctcgtt aagagagaaa aaaatcagga taaagtga
//
LOCUS       DAFNBJ010000264         3955 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.265, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000264 DAFNBJ010000000
VERSION     DAFNBJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.265"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(32..404)
                     /locus_tag="L2X49_004763"
                     /pseudo
     CDS             complement(32..404)
                     /locus_tag="L2X49_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(412..531)
                     /locus_tag="L2X49_004764"
                     /pseudo
     CDS             complement(412..531)
                     /locus_tag="L2X49_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(535..618)
                     /locus_tag="L2X49_004765"
                     /pseudo
     CDS             complement(535..618)
                     /locus_tag="L2X49_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611478.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            784..1311
                     /locus_tag="L2X49_004766"
     CDS             784..1311
                     /locus_tag="L2X49_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799716.1"
                     /translation="MHSFSELAYRCTNFSLAKINEGYDLSVKALSETGATPPLKNMQA
                     LNLQKMIHAVGLFSVYEAHLQRRLHCRDGFKEAKSILEQAGEISLKDKFHDYYLAINA
                     LKHGDGASYNNLVAKFNNLDFVVDTPTTPVQEEGDVSGVEGLVLVDDDFIQNCLAVIT
                     EVSECISKQRPDFLD"
     gene            complement(1389..2451)
                     /locus_tag="L2X49_004767"
                     /pseudo
     CDS             complement(1389..2451)
                     /locus_tag="L2X49_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611505.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2517..3485
                     /locus_tag="L2X49_004768"
     CDS             2517..3485
                     /locus_tag="L2X49_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HBN4799717.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVP
                     LRCPDYTSVSKRAKSVNVSFKTSPRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRI
                     WRKLHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRLSGSNARWKWTTEYNRRSIAETA
                     MYRMKQLLGDSLTQRDYDGQVAEAMAMVRALNRMTKAGMPESVRIA"
     gene            complement(3522..3779)
                     /locus_tag="L2X49_004769"
     CDS             complement(3522..3779)
                     /locus_tag="L2X49_004769"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799718.1"
                     /translation="MVIEAWLFDKPVHQAQSLNGVYHAVRLTTLSIGRSMQSDGKALS
                     EKSLKTGPVSFALSTLPICSGACSNVKISFENNHPKALLNK"
BASE COUNT         1018 a          954 c          924 g         1059 t
ORIGIN      
        1 tacggaggtg aggtgtgatt ctgttgtact aattcagaaa ataaccgcta gtttgttggc
       61 gggcctcgtt cttgcctgcg tagctattag ttggcaccca ttcttttttc atactgcgga
      121 agaaacgttc tatgggactg ttatccaaac agttttcccg tcggctgaca cttggcttaa
      181 tcctctaacg ccaaagaagt tatgatattt cagtcctgtg tactgacttc cttgagcgct
      241 ttgaaacatg acgtcctgcg gctgaccaca cacctcatac gccatccgta aggcaatgct
      301 tattagagca gtatcggtat ttgctaacag gctcctaccg ataacctttg agggaaaagg
      361 tctataacca ctaccagtga gaaccagcga tttcctgctg agagtagatt agtagaggct
      421 gtgtgtagag acgcccagac ggtcagatac ttcagcgacg gaataaccgc gctccgttat
      481 ctgacgggcg gcttcttcct taaattcagg tgtaaatcgt ggtgtgtcca tactgcggca
      541 gagttcacgg gcagaaaccc cggcttcagc ttcgcggagg atactgacaa tctgctcgtc
      601 ggaaaaacgc ttcttcatgg ggatgtcctc atgtagctta tgaagacatt actaacatcg
      661 cggtgtatta atcaacggag agcaggtcag gatacttaca ttttgagcac ctgatataga
      721 tcttatttca actacaaatt agaatcaata ctgttttgac taaacaggga ttttaaggaa
      781 cctatgcatt cattttctga gttagcatac agatgcacaa atttctcatt agcaaaaatt
      841 aatgaaggct atgatttatc agttaaagca ttatctgaaa ctggtgccac accaccttta
      901 aaaaatatgc aagctcttaa tctgcaaaaa atgattcatg cagtggggtt gttttctgtt
      961 tacgaagctc acttacagcg caggttacac tgtagagacg gtttcaaaga ggctaaatct
     1021 attctcgaac aggcaggcga gatttcgctg aaagataagt ttcatgatta ctatcttgca
     1081 atcaatgcgt taaagcatgg tgatggggct agctataaca atttggtcgc taaattcaat
     1141 aatcttgatt ttgtagttga tacacctact acacctgttc aggaagaggg tgatgtttca
     1201 ggtgttgagg gtttggtcct tgtggatgat gattttattc aaaattgtct tgctgtgata
     1261 accgaagtat cagaatgcat atcaaagcaa cgtcctgatt tccttgatta atgcgtggag
     1321 tttaaattcg aaatttttta tatgggctgg cgctgctgat ccattcccaa tatcggtgcc
     1381 aggttaaatc agagatccgg gcctttttgc agacttcgga taaattcctc cggtgtcagg
     1441 ttgtttaatg atgaatgagg tcgttgctgg ttatattcct gtctccagca ctcgatcttc
     1501 tcctgagcat cttccagtga caggaaccag tgcacattca gacattcatc acgaagtctg
     1561 tcattaaatg actcgaccag tgcattgtct gtgggctttc caggccgtga gaagtccatc
     1621 gtcaccttat tctcataggc ccatcggtcc agagatttgg agataaattc gctgccattg
     1681 tccgtctgca accggagtgg tacacggcgc ttcatttgct tcagacgctc cataacgacc
     1741 accacatcct caccacgtaa cccctgtcca acctggatcg ccagacattc ccgactaaaa
     1801 ttatcgacta tagccagcgc ccggatccgg cgtccgttga acagattatc agcaacaaaa
     1861 tccatgctcc agcactgatc tactgcgcta acttccggac ggacgcgccg gtgtctggca
     1921 gtgacatgtc ggcggggtcg ttttgtccgc agattcagtc cttccagaca gtaaatctga
     1981 tgtgttttct tatgatttat taaccagcct tcccgtcgta aaagaatatg aatacgctgg
     2041 caaccgtagc gaactcgcgc ttctgcaatc tctcttatcc tctgagtcag ggccctgtca
     2101 tcgcggcagc tacggtagtt gtataccgta cgactctgca tcatcagacg gcagccacgg
     2161 cgcagaccaa tccagcagat aagccaccgc ttcccgtttc tggacgggcc tcagaacttt
     2221 tttcgaatga catcctggag catttccttg tccagactga ggtccgccac caaacgacgg
     2281 aggcgctgat tttcatcctc cagttgccgc agacggcgca gttctgtcac gcccatcccg
     2341 ccaaacttca gaaatgccca tctttctgca cacttcttcc acccgagtgc cagtttcggc
     2401 ctgtttcagc gcaaaagcga tctgttcttc tgtatagcgg gtctttttca tgacggaatg
     2461 gcctctttgt ttgataaaag aaagggcttt gttgaataaa tcgaactttt gctgagttga
     2521 aggatcagat cacgcatcct cccgacaaca cagaccattc cgtggcaaag caaaagttca
     2581 gaatcaccaa ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct
     2641 ggctggatga tgaagcgatt caggcctggt atgagtcggc aacgccttca tcacgaggaa
     2701 ggccccagcg ctattctgat ctcgccatca ccaccgttct ggtgattaaa cgcgtattcc
     2761 ggctgaccct gcgggctgcg cagggtttta ttgattccat ttttgccctg atgaacgttc
     2821 cgttgcgctg cccggattac accagtgtca gtaagcgggc aaagtcggtt aatgtcagtt
     2881 tcaaaacgtc cccccggggt gaaatcgcac acctggtgat tgattccacc gggctgaagg
     2941 tctttggtga aggcgaatgg aaagtcagaa agcacggcaa agagcgccgt cgtatctggc
     3001 gaaagttgca tcttgctgtt gacagcaaca cacatgaagt tgtctgtgca gacctgtcgc
     3061 tgaataacgt cacggactca gaagccttcc cgggccttat ccggcagact cacagaaaaa
     3121 tcagggcagc cgcggcagac ggggcttacg atacccggct ctgtcacgat gaactgcgcc
     3181 gcaaaaaaat cagcgcgctt attcctcccc gaaaaggagc aggttactgg cccggtgagt
     3241 acgcagaccg caaccgtgcc gttgctaatc agcggctgag cggaagcaat gcacggtgga
     3301 aatggacaac ggaatataac cgtcgctcga tagcggaaac ggcaatgtac agaatgaagc
     3361 agttgttggg agattcactg acgcagcgtg actacgatgg tcaggtagcg gaagctatgg
     3421 ccatggtgcg tgcgttgaac aggatgacaa aggctgggat gccagaaagc gtgcgtattg
     3481 cctgaaaatc cagccagcta cagggtcgtt cgcacgaaat cttatttatt caacaaagcc
     3541 ttaggatgat tgttttcgaa ggagattttt acattactgc aagcgccgga gcaaatgggc
     3601 aatgtggaaa gagcgaagct tactggccct gtttttaaac tcttttctga aagcgctttc
     3661 ccgtcagact gcattgatct tccaattgat aaagttgtta acctgacggc atgataaaca
     3721 ccatttaggg actgtgcctg atgtacaggc ttatcaaata gccaggcttc tatcaccatt
     3781 cttcacgcct tgccggtgaa tcaaactcct tttctacaaa atttgcaggc tttgtataag
     3841 tagaaagtgg tgcagaacta agcgaaatct ccactcctaa aatatttaaa actttgtata
     3901 gacgctcaag gtaatgactc caacttattg atagtgtttt atgttcagat aatgc
//
LOCUS       DAFNBJ010000265         3918 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.266, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000265 DAFNBJ010000000
VERSION     DAFNBJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.266"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..3080
                     /gene="trhN"
                     /locus_tag="L2X49_004770"
     CDS             <1..3080
                     /gene="trhN"
                     /locus_tag="L2X49_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhN"
                     /protein_id="HBN4799719.1"
                     /translation="TPKIFAADAIFDQLENNFNLANPNANRNATTSAQDIVEKYKNAD
                     SGENLSGKISEKYVGKAESTNLNVGKYGTPNSNESVMSNAVSDGKSIGKAVQLPSMSG
                     GTINSNYTKEGAKLLSRDSSGNIGISNNPNTTAGTKTSTGELFSSEQKHSDVQFNAGG
                     RYGDENGFINDIKNRKSQLFEAQSYDGVAYRTLVNANKENPASTIKPNDPMFNAGRNE
                     IGNAVAGTGNWLQNCNTETSKQTITTHYPDYKEFYCNSPKKDNFNSCTITRDFSVPVY
                     ISGGNGDMSMCGDNCVRVWFGRRDDNYWNDGVYDNSLTLKFHPDAKLATAKIINAEWD
                     DHMRVTLDGTQIFAHIDGAYRSSNYPSPQGQWELKKSWKLDKVYDITEQVKTSVYQEQ
                     DREVTMASRVWVGGGGEGYFEVEMTFENMKLEDKHIQEPAGCFDAVQTPNSFCRFDRF
                     VNMDVGTKRLPESVLKMATPLYKGDTGYLTWKTNLEGYFCDPLAKEKLCSYDAKGNIM
                     KDPTGKDLCYNYDDIKNMPDACSTYKNDAACVLDNQTCAEGWFDEGTNTCYMYEQKYT
                     CDRGKDVVREVESQTNSCVGMIPCSGGTCETGPKEENKDFGKVAAYSNMVQYMQGEAK
                     CEDPNDPNSCSVFEGKAEWCGRSVGFVNGLAKTDCCEKPQGAAGSLEAIMLAGSMIRN
                     TNWTRVNAQLVNWTGGESGTWASMANSVGEWTASAGKTVGQMWNNVTSSITSVYENVA
                     GNLGRTVGSSAAGESGQLAKETMSSFGLGKLKQMAMEKAYDLLPDTVRDFVFKNVATT
                     GGEIVFSAAVQNFMLALNVIGWIYTAYQVTKMLLEMLVACDQKEMEASIHKNQKSCFT
                     LDTNRCVKYLNLGFTKKCVKKATDMCCYNSMLSRVIMQQAYPQLGIDPVTSNCVGLSI
                     GQIQKLDFDKIDLTEWINDAVQVGEVPDQYATFSESSITANLPFKNENYQLPSERTKD
                     AMGGEENMIKARQENAQAIKEENVDCSYLPRPAICEVGSTYVDPITGKEIPKY"
     gene            complement(3108..>3918)
                     /locus_tag="L2X49_004771"
     CDS             complement(3108..>3918)
                     /locus_tag="L2X49_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799720.1"
                     /translation="CAGNAGQSPNSAQHSDTHGLYWHANAKDSVAKNAYSLAGFDVSF
                     NEETVQPGGDADNFLSKLLISGSMGYVTGGLSGLSIMSLGSLYSSSDAEYIQVNQYVV
                     FVPNPKKLPYNDESLVRAGAAYVYNHTKESQVMLGFNPSKQSAALASCKIDRAVINKW
                     STCELTSKPAPDVMPTNSMYSFQAIRPATGTEIPQLNLPAGEYSVIRYVFIPFKGNES
                     SNNFSGIIFRSDSPKFTMPGGAAASINGKDYYLFSGEYGQKGFPEKTLKSK"
BASE COUNT         1236 a          765 c          962 g          955 t
ORIGIN      
        1 taacgcctaa aatatttgcg gcagacgcga tttttgacca gcttgaaaac aattttaacc
       61 ttgccaatcc caacgccaac cggaacgcaa ccaccagtgc tcaggatatt gtggaaaagt
      121 ataaaaatgc tgattccggg gaaaatctga gcggcaaaat ttccgagaaa tatgttggta
      181 aagcagagtc aacaaaccta aatgttggga agtacgggac gccgaactca aatgaaagtg
      241 ttatgagtaa tgcggtgtcc gacgggaaat cgatcggtaa ggctgtacaa ttacctagca
      301 tgtcgggcgg tacaattaac tcgaattata cgaaagaggg ggcaaagtta ctttctaggg
      361 acagcagcgg taatattgga ataagtaaca acccgaatac aacggcaggt accaaaacca
      421 gtacagggga actattcagc tcagagcaga agcacagtga tgttcagttc aatgctggtg
      481 gccggtatgg agatgaaaat ggcttcatca atgacatcaa aaatcgaaaa agtcagctgt
      541 ttgaagcgca aagttatgat ggagtcgcct accgtactct ggtgaatgca aataaagaaa
      601 acccagcatc tactattaaa cctaacgacc ctatgttcaa tgcgggtcga aacgaaatag
      661 gaaatgctgt agctggtaca ggaaactggc tacagaattg taatacggaa acatcaaaac
      721 aaacaattac tacgcactat ccagattaca aagagtttta ctgcaattca cccaagaaag
      781 ataactttaa cagttgcacg ataactcgtg atttttccgt gccggtttat atctcaggtg
      841 gtaatggtga tatgtccatg tgcggagata attgtgtgag agtctggttt ggcagaaggg
      901 acgacaacta ttggaacgat ggagtctatg acaactcatt aaccttaaaa ttccatccag
      961 atgcaaagct cgcaactgcg aaaataatta acgcagagtg ggatgaccat atgagggtca
     1021 cgcttgatgg tacacagatt tttgctcata tagatggagc atatagaagt agcaattatc
     1081 catctccaca aggtcaatgg gagttaaaga aatcctggaa gctcgataaa gtctacgata
     1141 ttactgaaca agtaaaaacc tctgtatatc aggagcagga tcgtgaagtg actatggctt
     1201 cgcgcgtatg ggttggcggt ggtggggaag gctattttga agttgagatg acttttgaaa
     1261 atatgaagtt ggaggataaa catatacagg agcccgcagg atgttttgat gcagtacaaa
     1321 cgccgaattc attctgtcgg tttgacaggt ttgtcaacat ggatgttggt acaaagcgac
     1381 tcccggaaag tgtgcttaag atggccacac cgctttacaa aggtgataca ggatacttaa
     1441 cctggaaaac aaaccttgaa ggctatttct gtgatcccct ggcgaaggaa aaactgtgct
     1501 cttatgatgc gaagggaaac atcatgaaag accccacagg caaagacctt tgctacaact
     1561 acgacgatat aaaaaatatg cctgacgcat gcagcacata taaaaatgat gctgcgtgtg
     1621 tgctagataa tcagacatgt gctgaaggtt ggttcgatga aggcacaaat acttgctaca
     1681 tgtatgagca gaaatataca tgtgacagag ggaaagacgt agtcagagag gtagagtcac
     1741 agactaactc ctgtgtaggt atgatcccat gttctggcgg aacatgtgaa acaggcccaa
     1801 aagaagaaaa caaagatttt gggaaagtgg ccgcgtactc caatatggtt cagtacatgc
     1861 aaggtgaagc aaaatgcgaa gatccaaacg accctaactc ctgctctgtc tttgagggga
     1921 aagcagagtg gtgcggcagg tccgttggtt ttgtgaacgg actggccaaa acagattgct
     1981 gcgaaaagcc gcagggtgct gctgggtcac tggaggcgat aatgctggcc gggtcgatga
     2041 tccgaaacac gaactggacc cgagtgaatg ctcaactggt aaactggact ggaggggaga
     2101 gcggaacctg ggcatcaatg gctaactcag tgggtgaatg gactgcatcg gcaggtaaaa
     2161 ctgtcggaca aatgtggaac aatgtgacca gttcaataac cagcgtttac gaaaacgtgg
     2221 ccggtaacct tggccgaaca gtaggatcat ccgctgctgg tgaaagtggt cagcttgcta
     2281 aggagactat gagttcattc gggcttggta aacttaagca gatggccatg gaaaaagcct
     2341 atgacctgct tcccgataca gttcgagact tcgtatttaa aaatgtagcg acaacgggtg
     2401 gagaaatagt cttttctgct gctgttcaaa actttatgct tgcattaaat gtaatcggct
     2461 ggatttatac ggcatatcaa gtcacaaaaa tgctgcttga aatgctggtg gcctgcgatc
     2521 aaaaagaaat ggaagcatct attcacaaaa atcagaaatc ctgttttaca ctggatacaa
     2581 accgttgtgt taaatatctg aatctgggat ttacaaagaa atgtgttaaa aaagcaacag
     2641 acatgtgctg ttacaattca atgttatctc gcgttattat gcagcaggcc tacccgcagc
     2701 ttggaataga cccggttact tcaaactgcg ttggcttatc aatagggcag atccaaaagt
     2761 tagattttga caagattgat ttaacagaat ggattaacga tgctgttcag gtcggagaag
     2821 taccggacca gtacgctaca ttctcagaat cgtcaataac agcaaatttg ccatttaaaa
     2881 acgagaatta tcaacttcca tcagaaagaa caaaagatgc aatgggagga gaggagaata
     2941 tgataaaagc cagacaggaa aacgctcagg ccattaaaga ggagaacgta gactgtagct
     3001 atcttccacg ccctgcgatt tgtgaagttg ggtcaactta tgtagatcca attacaggta
     3061 aggaaatacc aaaatattaa aaaaaagggg ctgtaaagcc ccttttgtta ttttgatttc
     3121 agagtttttt ccgggaatcc tttctgacca tactcaccag agaagaggta atagtctttc
     3181 ccattaattg aagcagctgc gccgcctggc atggtgaact ttggactatc agaacggaaa
     3241 ataatacctg agaaattatt gcttgactca ttgcctttaa atggaatgaa cacgtaacga
     3301 ataactgaat actcaccggc aggtagatta agttggggga tttcagttcc agtcgcaggg
     3361 cgaatagcct ggaagctgta catggaatta gttggcatta cgtcaggagc tggttttgaa
     3421 gtgagctcgc atgtactcca tttattgatg accgccctat caatcttgca ggacgccaga
     3481 gctgcacttt gcttcgatgg gttgaatccg agcattacct gtgactcttt ggtgtgattg
     3541 taaacatacg cagcaccggc acgaactagg ctttcatcgt tataaggaag tttcttcggg
     3601 ttcgggacaa agacaacata ctggttaacc tgaatgtatt cggcgtcaga ggagctgtat
     3661 agagagccca aactcataat gcttaatccg ctgagaccgc ctgtcacata tcccatagag
     3721 ccgctaatca agagtttgga aaggaagtta tctgcatcac cacctggctg aacagtttcc
     3781 tcattaaaag aaacgtcaaa cccggccaga gagtaagcat ttttggctac agaatctttt
     3841 gcgttagcat gccagtagag cccatgagtg tcgctgtgct gggcggagtt aggggattga
     3901 cctgcgttcc cagcacac
//
LOCUS       DAFNBJ010000266         3903 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.267, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000266 DAFNBJ010000000
VERSION     DAFNBJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.267"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..1267
                     /gene="ychO"
                     /locus_tag="L2X49_004772"
     CDS             <1..1267
                     /gene="ychO"
                     /locus_tag="L2X49_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBN4799721.1"
                     /translation="PDLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFAL
                     GKVRDALSQQVNQHVESWLSPWGNASIDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWS
                     QLGLTQQDDGLVSNVGVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRL
                     SANFYQPFAAWHEQTATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGERVDLFN
                     SGTGYHNPVALSLGLNYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLS
                     AGEVAESQSLRGSRYDNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIR
                     SRYGIRQLIWQGDTQILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQ
                     GQRVSSNEITLTLVEPFDALSNDELRWEP"
     gene            complement(1268..1918)
                     /gene="narL"
                     /locus_tag="L2X49_004773"
     CDS             complement(1268..1918)
                     /gene="narL"
                     /locus_tag="L2X49_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBN4799722.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(1911..3707)
                     /gene="narX"
                     /locus_tag="L2X49_004774"
     CDS             complement(1911..3707)
                     /gene="narX"
                     /locus_tag="L2X49_004774"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBN4799723.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
BASE COUNT          933 a         1002 c         1025 g          943 t
ORIGIN      
        1 gccggattta ggcatggctc ccgaaaatca tgatggggaa aaacactttg ctgaaattgt
       61 gaaagatttc ggcgaaacca gtatgaatga taacgggctg gatactggcg agcaggcaaa
      121 agctttcgca ttgggaaaag tccgcgacgc gcttagtcaa caggttaatc agcacgtaga
      181 gtcctggcta tcaccgtggg gaaatgccag tattgacgtc aaagtggata acgaaggaca
      241 tttcaccggc agtcgtggaa gctggtttgt gccgttacaa gataatgatc gttatctcac
      301 ctggagccag cttggtctta ctcagcagga tgatgggttg gtgagcaatg tgggcgttgg
      361 gcaacgctgg gcgcgcggca actggctggt gggttataac actttttatg acaacttgct
      421 ggacgaaaat cttcagcgag cgggctttgg tgccgaagcg tggggcgaat atttgcgact
      481 atcggcaaac ttttatcagc cgtttgctgc atggcatgaa cagacagcca cgcaggaaca
      541 acggatggcg cgcgggtatg acctgacagc ccggatgcgc atgccgttct atcaacacct
      601 caataccagt gtcagcgtag aacagtattt tggtgagcgt gttgatttgt ttaactctgg
      661 tacgggttat cacaatcccg tcgcgttgag tctgggatta aattacaccc ctgtgccatt
      721 agtcactgtg acggcccagc ataaacaggg tgaaagtggc gagaatcaaa ataacctcgg
      781 gctgaatctt aattaccgct ttggtgtacc gctcaaaaaa caactttctg cgggcgaggt
      841 tgccgaaagt cagtcgttac gtggtagtcg ctatgataat ccgcagcgaa ataatctacc
      901 gactcttgag taccgacagc gaaaaacgtt aacggtgttt ctggcgacac cgccgtggga
      961 tctaaaacct ggcgaaacag tgccgctgaa attacaaatc cgcagtcgtt atggtattcg
     1021 gcaactgatt tggcagggcg atacgcagat attaagtttg acgccaggcg cacaagccaa
     1081 cagcgcggag ggctggacgc tgatcatgcc tgactggcag aacggggaag gggcgagcaa
     1141 tcactggcga ttgtcggtgg tggtggaaga taaccagggg cagcgtgtct cctccaatga
     1201 gatcacgcta acgcttgtcg aaccgttcga cgcattgtca aacgacgaac tgcgctggga
     1261 accgtaatca gaaaatgcgc tcctgatgca cccataccgc tgcttccacg cgagacttga
     1321 gcttcatttt cttcagcatg tgcttgacgt gcacttttac tgtgctttcg gtgatgtcca
     1381 ggcggcgggc aatcatcttg ttcggcaaac cctgggcaat cagcttgaga atatcgcgct
     1441 cgcgtggggt taactggtta acatcgcgtt cagtagtggc acggttagcg cgcaagctgg
     1501 ctgccagaac aggcgttaat gcctcgctta ataccatttc gccagcagct gcctgatgca
     1561 atgctttcag cagatcttcc ggttccatat cttttaatag atagccatcc gcgccgcgtt
     1621 tcagtgcggt gaccacatct tcttcatggt tagagacgct gaataccaca atgcgccctg
     1681 agagggactt ttcgcgcagt ttatccagcg tttccagacc gttcatgccg ggcatattga
     1741 gatctaacag gatcagatcg ggatcaagag attccgccag ttcaataccc tgttcgccat
     1801 tactcgcttc gccaactacg gtgatatctg gtgccatact gataagctgt ttcacgccag
     1861 ttcgcagcat cggatgatcg tcgatcagca ggatagtagc cggttcctga ttactcatgg
     1921 gtatctcctt ggacgtctgt gaaagttttt tcgggaataa aggtgacaac cacttcggtg
     1981 ccacctgatt cacgacggcg aacgcggcaa tcgcctcgta aactttgcgc acggtcgcgc
     2041 attattatca tgccgtagtg attgctgcgg atggcatttt caggcacgcc gcagccgtta
     2101 tcctggacgg tcagtttgac ctgattatcg ttttgcgcca ccgtcaccac gacctcactc
     2161 gcttgcgaat gtttgagggc gttacttaat gcctcacggg caatttgcaa caagtggatt
     2221 gcctgatgcg aaggcaccag acgaggcggc aattgataat ccagcttcac cgggaagcca
     2281 aatttggcgc tgtactcttc gcaactcgcc tccagcgccg gacgtaatcc aggctcggtg
     2341 agctgcaagc ggaatgtggt gagcaattca cgcaactgcg cccaggatgc attcagttcg
     2401 ttacggatct gacttaacag ttcgcggctg ctttctggca gcgcatcgcc ctgcatctgt
     2461 aaacaactca cctgcatctt catgcaagag agagattggg caatagaatc atgcagttcg
     2521 cgcgcaatgg tggcacgctc ttccatcacg atcaactgtt gctgacgttc ctgatgccga
     2581 tccagcgcca gcgtggcggt gagttgttca accagggtat ccaccagttg ttgttgatca
     2641 tggctaagat gacgcccctg cggcagggtc gccagcaaaa taccgtactg cgtatgagag
     2701 tcagccagcc gccacttcag ggtcgtgccg cgatcaccaa cgggtaatac gccgcgcggg
     2761 cagagctggc agcctttatc atcacaagtc atatctggct ggcaggtaaa ctcctgatga
     2821 ttctcttcat catcagtgtc atacacccgc agttcgatat cacgtagcag ggttaaattc
     2881 tgtaagccgt tgagtacagg tgacaggcgt tcacacagcg gggcgcggga atgcaaacgg
     2941 cggttagcct gccataaaaa agagaggatc tgatttttat gctccagccc ggcggttttc
     3001 tcctgaaccc gctgctcaag tacggcataa ctttcggcca gttctgcaga catattgttc
     3061 aacgcagttc caagcatcgc catttcgttg cgcccgctga tgtttgcgcg ttgggtaaaa
     3121 tcgcgatggc tgacggcact cgccattgcc agcagttgcc gccacggttg tagcagtcgc
     3181 gcccgcaacc agataatagt gaacaccagt aaaagtgcca taaataccgc cattacccga
     3241 tggaccagta ccactgtctc aatgcgcatt tccgtggtgc ggtcaaaacc agataccagc
     3301 tgatcaagcc cggcaacaaa ctggctgaca tccgctgaca ccgtttctcg gttttgtgca
     3361 cgcatcagcg cagggatcag ttcattacgc cagtaatctt gtaaaccctg taattgcgcc
     3421 agttgtccgt ctcgttctgc tgctcgagtc aactcggcgc taaatgccgt ttgttccatc
     3481 tctttaatta agggcttgtc tttctcgctt aatggcactg ccgccaacag acggtaactt
     3541 tgcatgcgca gcgatcccgc tttgttgatc gcatgggcgc tgccctgaac gccttgcacc
     3601 agccagccag aaaccgccat ccctgccagt ccaatagcag tagaaagcaa cacaataagc
     3661 gcaacctgat taaccagggt gagcggagag agacaacgtt taagcatgta aacctcttcc
     3721 ttcaggcttt aaatgagcaa taaccttaat gaatgtgacg atacaatctg gaatggcagt
     3781 attctcggct attggctgaa gtatacccat acccggaaag agttactcct tatttgccgt
     3841 gtggttagtc gctttacatc ggtaagggta gggattctac agcaccgtga aaaatctcat
     3901 aat
//
LOCUS       DAFNBJ010000267         3887 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.268, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000267 DAFNBJ010000000
VERSION     DAFNBJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.268"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(7..2487)
                     /locus_tag="L2X49_004775"
     CDS             complement(7..2487)
                     /locus_tag="L2X49_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBN4799724.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(2503..3177)
                     /locus_tag="L2X49_004776"
     CDS             complement(2503..3177)
                     /locus_tag="L2X49_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBN4799725.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(3258..3800)
                     /locus_tag="L2X49_004777"
     CDS             complement(3258..3800)
                     /locus_tag="L2X49_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBN4799726.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         1082 a          956 c          722 g         1127 t
ORIGIN      
        1 tgactattac tggcaaatat agtttctact ttcatcaatc tctttaccga aggtgattgt
       61 gcatgaaagt ccttgttgct tatcaattgc tacattaacc gatggcggta cttcattggt
      121 gcgaataaat aactggcttc cctgaccgac aacgccaata ttatgaccat ggatatcatt
      181 gacttcataa ccaaacatta atggttgccc atccgctctt aacgctttta taaaccaggg
      241 cttgcgctga tcggtatcaa aattaaccag tacaaccgcg ccgcgataag gggcggcaat
      301 tttccggttg ccacgtaatt ctgcttcgct atcgctttgc gacacatcca gcatcaggtg
      361 attttcccga taaggtgtca ttccgtcgta taccaccact ccattacggt ttgttgtgcg
      421 atatttttgc ccattgacat aagcatcttt aattcctggc gcattcatca cagcaaacgt
      481 ttcggaaaga cggttcgcca gattaacgcc ccccgaccag gcgacaatgc cccctgaaac
      541 actggctcca gcctgtcgat aagtactcga ctgactataa ctgccattca ctgtcgcaac
      601 cggcgcgttc caggtcaaat tcgccccagc tgtcgtttca tttccctgat tctgataact
      661 caggttgacc ccatagttaa actgatcccg gcttccaacg gttcctgata atcccgtatt
      721 atttgaggca aacccctgat catcaaacgt cgttgagtta gacatatata tttgccgacg
      781 aggcgtcgta acgtcatcac cccaatcaaa gggaatcgat ataaaaatat taaaacgttt
      841 ctcttcatga tgattctcgt catacgcctg gcttgccgcg aggatatagc ttatccgccg
      901 ccagttattg gaataactca actgataatc cttactgctg ccgctacgcc cccagtaatc
      961 tcgccataac gtacttaagg acacagaccc ccagccttct ggcaatgact ggctcatatt
     1021 ggcagagaag ctatttttac ggccaaaatc gttctggtaa taatcggcaa tgtcatagac
     1081 atcgttttca tcacggcgat aattatcttt attgtttgcc caaacgtgat cattaaatgt
     1141 ccggtaatca cgcgatgaat aacgccaggc cgccagacca aaacgcgtcg acgtttggct
     1201 cacaaatttg ttgtaggcaa tttgataact ttgcccgtca aacacatcgc cgttgtcttg
     1261 tttactatgc gacttcgtgg catcgacgga aatggcacca aagcgtgtat tccagccagt
     1321 ccccaaagta aacgcgtaat aattattcgc gaccatcgag ccaccataca gcgtcaataa
     1381 attattaaaa ccatactgat aacccgcctg gacaaaatca ctttgtttgc tcgccccttc
     1441 aatatggcta cgacccgccg caaaatcata tttcgatacg ccgggttgca gcatatttgg
     1501 cactgctgca taaggcacca gataggtggt taccgagccg tccgcctctt tcacgctgac
     1561 atcaagatct gctccaccac cggccaactg caaatctgta atcgcgaacg ggccaggagg
     1621 aacctctttc tgataaacca caaaaccgtt ctgttcaata gttaccagcg cattactctg
     1681 agcaatcccc tgcacccgtg gcgtaaaatt ttgtttcgag ttaggcaaca tctgcatatc
     1741 acgaaacaac cgcacaccgc tgaagcgaac agaatcaaaa atatcgcttg atgtgtacat
     1801 atcacccacg cgaagcgtgc cgagaagttg ggcaaatcca cgttccagat acagggtatt
     1861 gcttttccac acccctggat tgttatttgt tttactgaaa ctggcatcag aatgcagttg
     1921 ccaccccagt aaatttaacc cgctgttaaa acgtacatat gtactcttac tattacccga
     1981 cgctttatag tcactgtaat actgactcac ataataagag gtataaaacg cattaatacc
     2041 ccgctcccag ttttccggtg gaacatagcc actttccagt tcttccaccc aggcctgcgg
     2101 gacactgaaa tcgagacgaa aaaccccgat atcccaggta tagctcccac cctgaacaag
     2161 ttgctcaaat gttaaacatt gcttaccgct ggcgaagtta tcgctattaa tgcctaaccg
     2221 cttgataact tctcttgata aacatgtttc ttgcgggttg tctttaacaa taatctcata
     2281 tttcccgcgc cattgcttat tgacataaat atcaatgtca tactgccccg gtaagggttg
     2341 attatcatca agacgaatat ttgcaacctg ctggtctttc attccaccta tcataaaatg
     2401 ggtatcaaag gtttcttcag ctgcataagc ttcaatgccg agcaataacg ctacgattgc
     2461 tgatgcaagt ggggtcattc tcaacatagg cagctcctgc aattaaattt tgtcactaat
     2521 atagttgccg tggtcatcga taatggtcag ataccagtta tttgcattat tacttttgac
     2581 attaacactc tgactttcta agggggcaat cattatagtt tcataattga ctttgacatt
     2641 attagctttg acatccgaaa tcgtcaccca attagctgag tcatttttta tcaccaatcc
     2701 attgccactg cgatttacca gtaacttttt aaaggtcgct ttatttaccg gagcaatacc
     2761 cgccggccgg taaaacaatt tgattctgtt ttgcatcgca aacttcagtg cattcttacc
     2821 ttcttgttct ggactatttg gtggaatatc cagaacattc agataaaaaa tgctttcttt
     2881 attagtgggc agtttattcg gcataatttt gatttttact tgctgcccgg aatttgcccc
     2941 tatttttgca actggtggcg ttaacatgaa aggaacctga attttttctg gtggtaacga
     3001 cgtatcgcca tcatcaatcc acgcctgcag cagcgaagaa cgatttccct ggttcatcaa
     3061 ctgcaccata acttctttat tttctgccgg gtaaataatg cgcgtcccgt agataacaat
     3121 tccggcatgt gcaggaagga ccatagaaaa aatgagtaaa gataataatc ctttcatctt
     3181 aatgcctctt aaattaaaaa gccgccatgg ggatagcggc catgtttatt acctggagaa
     3241 acttacaatt ttatttatta atcctgcgcc acacgcataa ccagagaaga taacacatca
     3301 cctgattgca ctgagttatc tgtcagtttc aggtattttg ctttaaacgg aatagcatat
     3361 tgaccttccg ctgttttata actctcaatg ttaaaaggtg cagaaacatc caggacatcg
     3421 ctgttatcac ctaaatagag atagtaaccc acggtatcgt tgctactggc tatcgcctta
     3481 tcgttaccgg ttattttatc gttggtaatc aacgagagtt ttacaccagc aacttctttc
     3541 gggcagctaa tttttaacat gctgctggtt acaccgactg catcatcata ggtagaatat
     3601 ttagctttac cttcaaagac agtggttggt acagtcggca tattaatact tactgaatca
     3661 ccacccgctt cgaatttaca cggagattct gcaatattcc ccttaatagt taatgttccg
     3721 gtatcaacgt ctgcagcaaa tactccagcg ctcataaaaa aagaagaaaa gacagcggca
     3781 gcaataattg aacgtttcat aatatgttcc ttaataaaat ttatttttgg ttgttaagag
     3841 tctgtatctc ttggaaacaa atttctcaca tcaaaaaagt tgccgca
//
LOCUS       DAFNBJ010000268         3885 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.269, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000268 DAFNBJ010000000
VERSION     DAFNBJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.269"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            290..1675
                     /gene="dcuC"
                     /locus_tag="L2X49_004778"
     CDS             290..1675
                     /gene="dcuC"
                     /locus_tag="L2X49_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBN4799727.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1716..2396)
                     /gene="dpiA"
                     /locus_tag="L2X49_004779"
                     /gene_synonym="citB"
     CDS             complement(1716..2396)
                     /gene="dpiA"
                     /locus_tag="L2X49_004779"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HBN4799728.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2365..>3885)
                     /gene="dpiB"
                     /locus_tag="L2X49_004780"
     CDS             complement(2365..>3885)
                     /gene="dpiB"
                     /locus_tag="L2X49_004780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HBN4799729.1"
                     /translation="DYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQ
                     RDTDFDYVVIGDRHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRA
                     KTPIFDDDGKVIGVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIR
                     RQMMGMEPKQIARVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQW
                     LGKPIAEVVRPADFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSK
                     DEISTLNAQLTQIKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQ
                     QLIDSLREAFADRQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVG
                     NLLDNAFEASLRSDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTR
                     ADEPGEHGIGLYLIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          894 a         1009 c          913 g         1069 t
ORIGIN      
        1 acctatgctc atattagatt attttttgag cgcacagccg cagctaacac cattgcaatt
       61 aacaaatttg catcaatcca ccatcaattt gcacacatta ttatgtgata attgccaacc
      121 gctaaatatg cgttttgtta tctatgtata aaaaaagcaa cttcaatgtc ttaatggcag
      181 tttttcttga ttttaatcag cattcatcgc caatttattg ggcatatttt ttccttaagc
      241 tttaggaatt ttttattatt tactttgggg cctggagaca ggaaaaatta tgctgacatt
      301 cattgagctc cttattgggg ttgtggttat tgtgggtgta gctcgctaca tcattaaagg
      361 gtattccgcc actggtgtgt tatttgtcgg tggcctgtta ttgctgatta tcagtgccat
      421 tatggggcac aaagtgttac cgtccagcca ggcttcaaca ggctacagcg ccacggatat
      481 cgttgaatac gttaaaatat tactaatgag ccgcggcggc gacctcggca tgatgattat
      541 gatgctgtgt ggatttgccg cttacatgac ccatatcggc gcgaatgata tggtggtcaa
      601 gctggcgtca aaaccattgc agtatattaa ctccccttac ctgctgatga ttgccgccta
      661 ttttgtcgcc tgtctgatgt ctctggccgt ctcttccgca accggtctgg gtgttttgct
      721 gatggcaacc ctatttccgg tgatggtaaa cgttggtatc agtcgtggcg cagctgctgc
      781 catttgtgcc tccccggcgg cgattattct cgcaccgact tcaggggatg tggtgctggc
      841 ggcgcaagct tccgaaatgt cgctgattga cttcgccttc aaaacgacgc tgcctatctc
      901 aattgctgca attatcggca tggcgatcgc ccacttcttc tggcaacgtt atctggataa
      961 aaaagagcac atctctcatg aaatgttaga tgtcagtgaa atcaccacca ctgctcctgc
     1021 gttttatgcc attttgccgt tcacgccgat catcggtgta ctgatttttg acggtaaatg
     1081 gggtccgcaa ttacacatca tcactattct ggtgatttgt atgctgattg cctccattct
     1141 ggagttcctc cgcagcttta atacccagaa agttttctct ggtctggaag tggcttatcg
     1201 cgggatggca gatgcgtttg ctaacgtggt gatgctgctg gttgccgctg gggtattcgc
     1261 tcaggggctt agcaccatcg gctttattca aagtctgatt tctatcgcta cctcgtttgg
     1321 ttcggcgagt atcatcctga tgctggtatt ggtgattctg accatgctgg cggcagtcac
     1381 gaccggttca ggcaatgcgc cgttttatgc gtttgttgag atgatcccga aactggcgca
     1441 ctcttccggc attaacccgg cgtatttgac tatcccgatg ctgcaggcgt caaacctggg
     1501 tcgtacccta tcacccgttt ctggcgtagt cgttgcggtt gccgggatgg cgaaaatctc
     1561 accatttgaa gtcgtaaaac gcacctcggt gccggtgctt gttgggctag tgattgttat
     1621 cgttgctaca gagctgatgg tgccaggaac ggcagcagcg gtcacaggca agtaaatagt
     1681 aatgccggag gcaagtttcc tccggcttta tcatgtcacc cactgtggta tatgcgttgt
     1741 ggtctgccaa ctttgccgtg aacaatttca gcaataatca gatggcggct ggcgcaatat
     1801 tcaagataac gcctggcagt ggtgcgactg atggtcagtg cctgcgccac cgtttctgcc
     1861 gtatgttgca caccaggctc tttaaacagt tttcgcaccg cgtttagcgt caagggatca
     1921 atgccggtcg gcagttcgtc tttaggttca ccgcgagcat aggcattaaa catctcatca
     1981 atttgcttct ggctggcgct atcaatactt tccagcatat gtttacgctg gcggaaacgg
     2041 gttagcgttt gccccagccg ttcataggca atgggcttaa tgagataatc aaatacacca
     2101 caacgtacgg cttcagacac cgtttccata tcgctggctg cagtggtaaa caccacgtcg
     2161 ccgggataat gcgcctgcac cagttcatgc aataaattaa tccctctgcc gtcaggaaga
     2221 tagttatcga gcaagattag ccccggctta aaacgctcga tcatcattcg ggcctgcgcc
     2281 aggtttcccg ccagtaatat ctgactgaat ccgggaatgt gacgaatata ttccgcatgc
     2341 atctctgcca gcggcgtttc gtcctcaacg atcaataggg ttaatggagc tgtcattagg
     2401 tttcactttc ggaatataga ttgaaaacaa ggtaccgcag ggatcattat cttcgagagt
     2461 gataacacca ccgcagcgcg ttacgtagct ggcaatcaag tacaacccaa tgccatgttc
     2521 accgggctcg tcagcacgcg tactgacccc ctgctcaaat attttgtctc gtagagactc
     2581 tggaacgccg cagccctgat cggcgacttc aatcaccaca tcatcgcctt catcgctgag
     2641 gaataattca acgatcttgt ttccttcatc gctacgcagg ctggcttcga aggcgttatc
     2701 aagtaaattg cccacaatgg ctgcaaactc ggtgctatcc agtcctggcg gcagttgcga
     2761 aagctggcta ccggggacaa tgatcatttt tagccccagt tcccgggcgc gctgcacttt
     2821 accaaaaagc agccccgcca cctggcgatc ggcaaacgcc tcacgcaggc tgtcaataag
     2881 ctgttgctgg gcctgagact ccccctgcac catcgccagc acgcgatcat actctttcat
     2941 ctgcaacaga ccattgagcg tcgacatcca attgagatgc tcgtgtcgca atgtacgaag
     3001 gctctcgacg tattgtttta tttgcgtcag ttgcgcattg agggtggaaa tttcgtcttt
     3061 actacgaaag ctgataatgg cccccagcaa atcatcacct gaacgaatag cttcccgatt
     3121 ggcaataacg ctcagaccgt taaagttagc caccacatcc tgacgttttt catcaatctg
     3181 ttcggtaaag aaatcggcgg gcctgaccac ttcagcaatg ggtttaccca accattgccg
     3241 tccgggagag ctcagcccca gcatctttct tgcgttacga ttgatggcgg taatgtaacc
     3301 atgcggatcc accgcaatca gcccttcata aaccgaacta aacagcgcct cttgctgacg
     3361 gaccacgcgt gcgatttgct ttggctccat gcccatcatt tgccgacgga tatgcgctgc
     3421 caggaaccac gacagcaaca tcagaatccc taacagcacg acaaacacgc ctgccatcgg
     3481 taataaaaac tcagcccgcc agctatcgat tttactcacc aggtagccaa tcgacaccac
     3541 gccgatgact tttccatcgt catcaaagat tggcgttttg gcgcgcatcg ccatccccat
     3601 tgaccctttc ccggtaatga agtagctctc ccctttctcc agcgcgccct gtttggtgaa
     3661 ctgcatagga taaccaattt tctccggatt aggatggtaa aggcggatcg agtgccggtc
     3721 cccaatcacc acataatcaa aatcggtatc tctttgtaat ttgttagcga tggtcgccag
     3781 ccgtttgtag tcacgcgttt tcaccgcaga gatgacactg tcattggagg caataatttt
     3841 cgcctgattc attgccatgt cgcgtacatg aagcgttaaa tagtc
//
LOCUS       DAFNBJ010000269         3872 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.270, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000269 DAFNBJ010000000
VERSION     DAFNBJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.270"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(1137..1403)
                     /locus_tag="L2X49_004781"
     CDS             complement(1137..1403)
                     /locus_tag="L2X49_004781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799730.1"
                     /translation="MAFNPLNRQTMEESWLRTVAFNPLNRQTMEESWSCPVVFNPLNR
                     QTMGGSWSGTVAFNPLIGRPWRDYCRVPGHLTTYSSVHDGMLVA"
     gene            1553..2725
                     /gene="istA"
                     /locus_tag="L2X49_004782"
     CDS             1553..2725
                     /gene="istA"
                     /locus_tag="L2X49_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBN4799731.1"
                     /translation="MLSREDFYMIKQMRQQGVYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRRFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFSLEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            2725..3522
                     /gene="istB"
                     /locus_tag="L2X49_004783"
     CDS             2725..3522
                     /gene="istB"
                     /locus_tag="L2X49_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HBN4799732.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERCENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT         1017 a          937 c         1013 g          905 t
ORIGIN      
        1 gggggttaaa tgccacggga cacgaccagc tttcctccat ggtctggcga ttaagggggt
       61 taaattccat gggacacgcc cagctttcct ccatggtctg gcgattaagg gggttaaatc
      121 ccacggtacc cgaccagctt cccgccatgc tctggcgatt aagggggtta aatgccagga
      181 tacgcgacca gcattcccca tggtctggcg aataagggag ttaaatacca cggtacctga
      241 ccagcatgac cccatgtcct ggcgattaaa gggttaaatg ccacgctacg cgaccagcct
      301 ccccatggcc tggcgattaa gggggttaaa tgccacggta cccgaccagc ttgcctccat
      361 ggtctggcga ttatgggggt taaatcccac ggtacccgac cagcaggcac cgtggtcatt
      421 ggctaaaggg gaaattgcct cggcttgcag acaggacaac gtgacgccct agcggttaag
      481 tgggtttaat acgacggtac acaataagta tcccaccaga atctgtctat tgagtggatt
      541 agattaaaag atattcggcc tggatctcga cttagaatga cgaaatattt ggcaaaaaaa
      601 tagggtattc ggcaaatcct gttgttagga catcccgaat acccaaaaaa tatttggcaa
      661 tcaatatgaa ttgcccatac ccccaattga caataagaga gttttttggc aattcgtata
      721 attaggccaa ttcatccgtg tcgtctattg cactaacgaa gtattccgtg cggtagtcat
      781 ctccccccct caactagact gaaggtgatc catcttttgc aagcgtacgg tcacctgaca
      841 tgctgtccgc tcgtacagga attgtcatgc attctttaat ggtatctgct tcagccacgt
      901 gtccagtaca ctggttttca aatccagcaa ctactacata cgcatccggg tcgagccttt
      961 taagctggtc tataagctct tttactttta tgggttacct ctacgttttt gtatatttta
     1021 tcacgctgaa cggaagggcg aataactgta gtgaaacaaa aaaagttttg gccggaaaac
     1081 cttttagact ggctttcctt tagcaaagaa ctggtgaata ggggagcaat tccgcgctac
     1141 gcgaccagca tcccatcgtg tacagacgag taagtggtta aatggcctgg tacccggcaa
     1201 taatccctcc atggtctgcc gattaagggg ttaaatgcca cagtacccga ccagcttccc
     1261 cccatggtct ggcgattaag ggggttaaat accacgggac acgaccagct ttcctccatg
     1321 gtctggcgat taagggggtt aaatgccacg gtacgcaacc agctttcctc catggtctgg
     1381 cgattaaggg ggttaaatgc cacgggacac gaccagcttt cctccatggt ctggcgatta
     1441 agggggttaa attgttagcg ccagtgatat aagacggtaa ttcaccattt agattgtccg
     1501 ctccacccaa catgttgttt ccttaaggtc ccacaacaaa aaggacatca acatgctgag
     1561 cagagaggac ttttacatga taaagcaaat gcgccagcag ggcgtgtaca ttgtcgatat
     1621 tgcgactcag attggttgct ctgaacgaac ggtcagacgc tacctcaaat accctgaacc
     1681 gccagccaga aaaacccgcc acaaaatggt taagctgaaa ccgtttatgg attacatcga
     1741 catgcgcctg gcagagaatg tctggaatag cgaggttatc ttcgcggaga ttaaggcaat
     1801 gggctatacg ggcggacgtt ccatgctgcg ttactacatc cagcccaaac gaaaaatgcg
     1861 tccgtcaaaa agaacagttc gcttcgaaac tcagcctgga taccagctcc agcacgactg
     1921 gggcgaagtt gaggtggagg ttgccgggca acggtgcaaa gttaactttg cggttaatac
     1981 gctggggttc tcccgccgct tccatgtctt cgccgcacca aaacaggatg ctgagcatac
     2041 ctacgaatca ctggttcgcg ccttccgcta cttcggtggt tgtgtgaaaa cggtgctggt
     2101 tgataaccag aaggctgcgg tgctgaagaa taacaacggg aaagtcgtgt tcaactccgg
     2161 attcctgttg ctggccgacc actataactt cctgccacgg gcatgccgtc cacgcagggc
     2221 cagaacaaaa ggtaaggttg agcggatggt gaaatacctc aaggagaact tcttcgtccg
     2281 gtaccgcagg ttcgacagct tcactcatgt caatcaacaa ctggagcaat ggatagccga
     2341 tgtggctgac aaacgggaac ttcgccagtt caaagaaacg ccggaacagc gcttctcgct
     2401 ggagcaggaa catctgcagc cgttaccgga tacggacttc gataccagtt acttcgacat
     2461 ccgccatgtg tcctgggaca gctatatcga ggttggtggt aatcgttaca gcgttcccga
     2521 agcgctgtgt ggtcagccgg tatcgatacg aatatcgctg gatgacgagt tgcggatcta
     2581 cagtaatgag aaactggtgg cctcacatcg cctctgttcg gcatcgtctg gctggcagac
     2641 agtgccggag catcatgccc cgctctggca gcaggtcagt caggtggaac atcgaccact
     2701 gagtgcctat gaggagctgt tgtgatgcat gagctggaag tcctgctgag tcgcctgaaa
     2761 atggagcatc tgagttatca cgttgaaagc ctgctggaac aggctgctaa aaaagagctg
     2821 aactaccggg agttcctgtg catggcgcta cagcaggaat ggaacggcag gcatcagcgc
     2881 ggtatggagt cccggctgaa gcaggctcgt ctgccgtggg tcaaaacgct ggagcagttc
     2941 gactttacct tccagccggg catcgatcgt aaggttgtcc gggaactggc tggtctggcg
     3001 ttcgtggagc gctgcgaaaa cgtgatcctg ctgggacctc ctggtgtcgg aaaaactcat
     3061 ctggccatag ctcttggcgt aaaagcggtg gatgcgggac atcgggtact gtttatgcca
     3121 ctggacagac tgatcgcgac actgatgaaa gcgaaacagg aaaaccggct ggagcgtcag
     3181 ctacagcaac tgagttatgc ccgggtgttg atcctggatg aaataggtta tctgccgatg
     3241 aacagagagg aagccagcct gttcttccgg ctactgaacc gtcgatatga aaaagcgagc
     3301 atcatactga cgtcaaacaa agggttcgca gactggggag aaatgttcgg agatcacgtg
     3361 ctggcaacag cgatcctgga ccggctgcta catcactcaa ccacgctgaa tatcaaagga
     3421 gagagttacc ggttaaaaga gaaacgtaaa gcaggagtgc tgaccaaaaa cacaacgcca
     3481 atcagtgatg atgaaatggt ggaaagcgga cagcatcagt aacgaaagta tcttagcggg
     3541 catgaaaatg gcaaataacg gttaaaaatc gtggcgttga caaaattcca tgggacacgc
     3601 ccagctttcc tccatggtct ggcgattaag ggggttaaat gccacggtac gcaaccagct
     3661 tccctccatg gtctggcgat taagggggct caatgccacg gtaccctacc aactttctac
     3721 catggtcagt cgattcaagt ggttaaattt cgttattgaa taagcttaaa atggtttctt
     3781 attccaacca gaaagttaga tggttaagtt attatggaga taagacctga tcttacttgt
     3841 tttttggttg ttttaacaaa ccattcacct ta
//
LOCUS       DAFNBJ010000270         3837 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.271, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000270 DAFNBJ010000000
VERSION     DAFNBJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.271"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            178..1323
                     /locus_tag="L2X49_004784"
     CDS             178..1323
                     /locus_tag="L2X49_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN4799733.1"
                     /translation="MTEQTSPAQLDLDSAAQPPRVYIRGDWVLAHYCVLEPRVAQLRA
                     NMPSGGVFDLNQLGALDTAGATLLALLLGEDRVANLRELAPKLPEERRILLETVSHVL
                     PDLTPELSEKPTRFWLEMLANTGRSVDNLWQDIKSLLGFIGLALETLLGTLFRPSRWR
                     ITSLIANIQQIGLNAVPIIMLLTFLVGAVIAFLGATVLTTFGAGIFTVDLVVFSFLRE
                     FAVLLTAILMAGRTASAFTAEIGLMKANEEIDAIQTLGLNPVELLVLPRVLALLISLP
                     MLTFIGMVCGIFGGMVVCALTLDISPTMFLSIMQNSNGLQHFLVGISKAPIFAFLIAI
                     IGCLEGFKVTGSAESVGVHTTTSVVHSIFVVILLDAVAALFFMEMGW"
     gene            1329..2120
                     /locus_tag="L2X49_004785"
     CDS             1329..2120
                     /locus_tag="L2X49_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN4799734.1"
                     /translation="MTQDAIIQIRDLVNCFGVQCVHQGLNLDVKRGEVLGVVGGSGTG
                     KSVLLRSIVGLRRPTDGKIHVFGKDLMALSGQSRAMVERRFGVLFQRGALFSSLTVTE
                     NVALPLIENAGLPRSEAERLAQVKLALAGLPPGTGSKYPASLSGGMVKRVALARALAL
                     DPDILFLDEPTAGLDPIGAAAFDSLIRTLRDALNLTVFLVTHDLDTLYTLCDRVAVLS
                     QKKVLVVDTLDKVAATDDGWIQAYFHGPRGRAAYQAAAINSGERL"
     gene            2122..3060
                     /locus_tag="L2X49_004786"
     CDS             2122..3060
                     /locus_tag="L2X49_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HBN4799735.1"
                     /translation="METRAHHVVIGLFTLIVVSAALLFCLWLTNSGSDRQFRLYDIVF
                     NEPVSGLSQGSTVQYSGIRVGEVTQLRLDRDNPNKVWARIRVSASTPIREDTQARLTV
                     AGITGTSNIQFSSGSQTSPLLEGKDDEVPIIIATPSPMSQLLANGENFMTNANEVLVR
                     LNQLLAPDNQQRLVTTLDNLALVTQTVADQRGDLRTLLQQLAQASKQANETLTQTNRL
                     LRNANGLMDGQGKQLVSDAAKTMASLQNTSTILNKLVSENKTSLNNGMQGMNELGPAV
                     DELRRTLATLRAAVSRLEENPAALLRGRERTQEFTP"
     gene            3060..3701
                     /locus_tag="L2X49_004787"
     CDS             3060..3701
                     /locus_tag="L2X49_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBN4799736.1"
                     /translation="MLSFNVMRYAGRRVWLLMISGGAILLSACTILPSAPVSQVYLLP
                     VPPAVSVPHAQTVNWSLRVSQPATNQFLNSSRIAVQPQGQEIAVYKNSRWSDSAPILV
                     RNRLIQAFRSDGRIRAVSSDDDSLQADVELSGDLSAFQGVYHVGSSEVLIRFDARLVR
                     ISDRRIIATRQFDIRQPIKGNEMNEVVQAFGLASDQLATQVLNWTMQQSPAQG"
BASE COUNT          854 a          880 c         1047 g         1056 t
ORIGIN      
        1 ttggtttacc ctgccagaga ggaaaatacg gcaattgggc aaacagcgtg ccacacctca
       61 ctgattaggg tgtggttaac acttcattaa ttaaacagtc attactctta cttaactgtg
      121 tgatatacct atttagggtt tgaaacatta gagtttgaaa taggtaaggt ctgagcaatg
      181 actgagcaaa cgtcccccgc acaacttgat ctcgatagcg cggctcagcc ccccagagtt
      241 tatatccggg gggactgggt gttggcgcat tactgcgttt tggagccgcg cgttgcgcaa
      301 ttgcgcgcta atatgcccag tgggggggta tttgatctca accaactggg ggcgttagat
      361 accgccggtg caacgttgtt agctctgctt ctgggtgagg accgtgtagc aaatttacgc
      421 gaacttgcgc ctaaattgcc agaagagcgg aggatattac ttgaaacggt aagccatgta
      481 ttgcctgact taacccctga gttgtctgaa aaacccacac ggttttggct tgagatgctg
      541 gctaataccg gccgttcggt agataactta tggcaagata ttaagtcact attgggcttt
      601 attggtctgg cgctggaaac cttgctggga acactttttc gcccctcccg ctggcgcatt
      661 acctcattaa ttgccaatat tcaacagatt ggcctcaatg ctgtgccgat cattatgttg
      721 ctcacctttt tggtgggggc agttatcgcc tttctcggtg caaccgtact cactactttc
      781 ggcgcgggca tttttaccgt cgatttggtg gtattttctt ttctacgtga atttgcggta
      841 ctgcttaccg cgatcctgat ggcagggcga acagccagtg cttttaccgc tgaaattggc
      901 ttgatgaaag ccaatgaaga gattgatgct atccaaacac tgggcttgaa tccggtcgaa
      961 ctgttggtgc taccgcgcgt cttggcacta ttgatctctc tgccgatgct aacctttatt
     1021 ggcatggttt gcggtatttt cggtggcatg gtggtgtgtg ccttaacact ggatatctct
     1081 cccactatgt ttttgtcgat tatgcaaaac agtaatgggt tgcagcattt tctggtggga
     1141 ataagtaaag cccctatttt tgctttcctg attgctatta ttggctgtct ggaggggttc
     1201 aaagtaaccg gtagtgctga atcagtcggg gttcatacca ccaccagtgt ggtgcattcg
     1261 atctttgtgg tgattttgct tgatgccgtg gcagcgctat ttttcatgga aatggggtgg
     1321 tgagccaaat gacgcaagat gcaatcattc aaattcgcga tctggttaac tgctttggcg
     1381 tacagtgcgt gcatcaaggg ttgaatcttg atgttaagcg cggcgaagtt cttggcgtgg
     1441 ttggtggctc gggtaccggt aaatctgttc tgttacgcag tattgttggg ttacgtcgtc
     1501 cgactgacgg gaaaattcat gtttttggca aggatttaat ggcactgtca ggtcaatccc
     1561 gcgccatggt cgagcgccga ttcggcgtat tatttcaacg aggcgcactg ttcagttctc
     1621 taaccgtgac cgagaacgtg gcattaccat taatagaaaa cgccggatta ccccggtctg
     1681 aggctgagcg attggctcag gttaaactgg cactggctgg gctaccgccg ggcactggca
     1741 gtaagtatcc agcatctctg tcgggtggta tggtcaagcg tgttgcgcta gcccgtgcct
     1801 tagcactgga cccggatatt ctgtttcttg atgaaccgac tgcgggtctt gatcccattg
     1861 gtgcggcggc gtttgacagt ttgatccgca ccctgcggga tgccctcaat ctaacggtat
     1921 tcttggtgac ccacgatctg gatacgctgt atacactgtg tgatcgtgtg gcggtgcttt
     1981 cgcagaaaaa agtgctggtg gttgatactc tggataaagt tgcagcgact gatgatggct
     2041 ggattcaggc gtattttcat ggcccacgtg gccgggctgc ttatcaggca gcggcaatta
     2101 acagtggaga gaggttgtaa tatggaaacc cgtgctcatc atgtcgttat tggtttgttc
     2161 actcttattg tggtgagtgc cgcgctgctg ttttgcctat ggctgacaaa ttccggttct
     2221 gatcgccagt tcagattgta cgatatcgtt ttcaatgagc ctgtgagtgg cctgtcacag
     2281 ggtagcaccg ttcagtacag cgggattcga gtcggtgagg tgacgcaatt acgcctggac
     2341 cgagacaatc cgaataaggt ttgggcacgt attcgcgttt ctgcatctac tccgatacgc
     2401 gaagataccc aagctcgtct caccgtcgcg ggtattaccg gcacctcaaa tattcaattc
     2461 agcagtggca gccagaccag cccgttgctg gagggcaaag atgacgaggt tcctatcatt
     2521 attgctaccc cttcgccgat gagccagtta ctggctaacg gtgagaattt catgaccaat
     2581 gcgaatgaag tgttggtgcg tttgaaccag ttgctggcac cggataacca gcagcgccta
     2641 gtaacgacac tggataatct cgcgctggtc acccaaactg tggctgatca acgtggtgat
     2701 ctccgtaccc tgttgcagca gttggcgcag gccagtaaac aggctaatga gacactgact
     2761 caaaccaatc gcctgctgcg taatgctaac ggcttaatgg atgggcaggg gaaacaactg
     2821 gtcagtgatg ccgccaaaac catggcatcg ttgcaaaata ccagtaccat cctcaataaa
     2881 ttggtgagtg aaaataaaac ctcactcaat aacgggatgc aagggatgaa tgagctgggg
     2941 ccggcagtag atgagctgcg cagaacatta gcgacattac gtgccgccgt ttctcgtttg
     3001 gaagaaaacc cagcggcctt gttgcgcggc cgtgaaagaa cacaggagtt tacgccttaa
     3061 tgctctcttt taatgtgatg cgctatgcag gccgccgcgt gtggctattg atgatatccg
     3121 ggggggcgat actgctctct gcctgcacca ttttaccttc ggcccctgta tcacaagttt
     3181 atcttctgcc ggtgccacct gcagtcagtg tgccacacgc gcagacagta aactggtcat
     3241 tacgggtttc acaaccggcg acgaaccagt ttcttaatag ctcacgcata gcggtgcagc
     3301 ctcagggcca ggagattgcg gtttataaaa actcccgttg gagtgattcg gcgccgattt
     3361 tggtgcgcaa tcgtcttatt caggcgttcc gtagcgatgg gcgaatccgt gcagtcagta
     3421 gcgacgatga tagcttgcag gctgatgtgg agttgagtgg tgatctgtcc gcgttccaag
     3481 gggtatacca tgttggtagc agcgaagtgc tgatccgttt tgatgctcgg ctggtaagaa
     3541 tttcagatcg taggattatt gcgactcgcc agtttgacat tcgccaaccg ataaaaggta
     3601 acgagatgaa cgaggtggtt caggcctttg gcctggccag tgaccagtta gccactcaag
     3661 tgctcaactg gacgatgcag caatcccctg cgcagggttg atatctaaac cagcaccggt
     3721 tggttttatc tgaggcgagt tggcagtagc taactcgcct tttttatcgt ctagtttaat
     3781 gattcaaggc gaataattgc gtctaagggg aaaatattct ctttattgtt ttgtgat
//
LOCUS       DAFNBJ010000271         3731 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.272, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000271 DAFNBJ010000000
VERSION     DAFNBJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.272"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(585..839)
                     /locus_tag="L2X49_004788"
     CDS             complement(585..839)
                     /locus_tag="L2X49_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HBN4799737.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNC
                     LVVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
     gene            complement(1011..1694)
                     /locus_tag="L2X49_004789"
     CDS             complement(1011..1694)
                     /locus_tag="L2X49_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HBN4799738.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            complement(1948..2481)
                     /locus_tag="L2X49_004790"
     CDS             complement(1948..2481)
                     /locus_tag="L2X49_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HBN4799739.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            complement(2923..3210)
                     /locus_tag="L2X49_004791"
     CDS             complement(2923..3210)
                     /locus_tag="L2X49_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HBN4799740.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            complement(3128..3391)
                     /locus_tag="L2X49_004792"
     CDS             complement(3128..3391)
                     /locus_tag="L2X49_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBN4799741.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
BASE COUNT         1023 a          822 c          905 g          981 t
ORIGIN      
        1 ggtatgtaat aactgaaaaa ctcaaatcat aagatttcag tctctttata agtttattta
       61 ttcttgctgg agtaaagaga tgtgaatgca tgacaatgat ttcatcatta ttaattgctt
      121 ttgatatcag agaaagcaat acatcccagt gtttcctcat tgacttcggg caacgtgtgt
      181 cagcataggg gctaagatgt atagttgatt tcttgcttcc ctttttcttc ttcacaaaga
      241 atgtgaagtc gtatttagat gcagacctta caataagaag tacacatctg aaaagtagtg
      301 ggaaaatgca aatcacaacc accacacaga aatataacat ataaggcaat gataagttgt
      361 cagtgtatgg ataacgtaag tattgccatg gggtagtgat gtacagacaa agcgtacacc
      421 aaagcaacca gagatataat ggtacgttat tatttttcat gttggctgag gcaggcgact
      481 aaaataacag actaaggagg attaactgtt taattctttg gaacaaaaaa atgcagttcc
      541 tgatcatcac acaccattga ataaacattt tgcttcggtt gcgttcatga tagccagtcc
      601 tgtagatgct gatggcggga gctgctgccc ttgtgccagc ggcagtggct gccgggagca
      661 cgctccagtc ggaacagaaa tcggttgtcg cgtagtacca ccacgagaca gttatccggc
      721 gtgcagaaac gtcgggcaag ctcatcgggg tctgtcggtg ttttcgttga aaacagcctg
      781 cgtttgcagt gtgcgtcgac gaccagtatt tgcagtcgtc cgggatgttg ctgtggcatc
      841 aaagactcct tccggaacgg gaaaagaagc agctgtaccg tcataactgc acgtaccggt
      901 tgcgaaaaaa cggcacaagc gagtagtgac agaaagaatg tcggaaacgg caggaagggc
      961 ggtacaggct gcgaagactg gaaacggggt atgccgggga ttacacatcc tcagtgtaat
     1021 gtgatagtcg tctgctcctg tgttgcaccc cagacatctt ctggcaagct gggcagcccg
     1081 ataaataggg tgtgaaagag ggtttctctg cgttcgatcc cgaattgatg ttcgtatcct
     1141 ctcaccacct gaaatccttc gtcggagaat attaactctg ccgtttccag aagctgcata
     1201 gttttgcggt gtagtggagt gatcagctgc aacgtaactg gtgccctcat atcgcctgtc
     1261 ggtagaaaac actcttcggt gcgacttagt tgtagcagat ggcgtttccc ggaacaggcc
     1321 cgttcaatgg attcattgaa caccctctca ggccatgggc agacataagt gcgccagcct
     1381 gctttctgta atttacccag cgcagcccga aaacgaaaca acggacattc cccgacagtg
     1441 gcagagctga gaaagagcgt attcagcgtt ccgatgatgg gaatattgcg atctttttcg
     1501 gtcaatgctt ttaacgccat gatttcgcca gggagactac ggaactcagg atgtctgctg
     1561 gcaccagaaa gccattcaag caggtaatgc cgtaatccgg tttcactggt tatattgtgg
     1621 ttttcctgtg ccgttttcag ggcattcagt gcacaccaga gcaactggcg cacgggatgt
     1681 tctgtcgtgg tcatgcgaat gtcctactgt gagatgaaaa gaagaccggt atccgttctc
     1741 tccgccacac gtgatggcac gggccggcct ttgataaaac gaatcaatac tctggggtga
     1801 agccttttat ttctttcgcc attaagaaat acacggttcc atcaaaagac agggggagtc
     1861 catggtgggg atggcttcgt gcaggcttca ctccggaata ttctcttgaa ttgaaaaaag
     1921 cccggcccga aggccgggaa atactggcta tgggatgttt acaaggcgct ccataaagcc
     1981 gtgatgttcg gcatttgata ccagttccag gagcagggat atcctcgact caggctggag
     2041 agtattttcc attctgagga gatactgata ttgtgctttt gtcagccata tgctggaagc
     2101 cgcattcagc tcttccctgg ctcccggatt tagcacaggg tccgggtaaa tcttcattaa
     2161 tccatcaaca atatctttat tgacgggttt tatttcaccg ccaaacttca cgaaatcaat
     2221 gaaggcgcta tggcgacgta atgccgatac tggttgaatg tcaaaattag ccagaataaa
     2281 aaagtaccct ggaaaaatag cgaatattct ttcacggtaa cagcacctgg agtctgcccg
     2341 tcttatcttt cttgttatta atggagtcca tggtacgacg ttttgctcat tcagccatga
     2401 gaataaagat tctctgtttt tgccggctgt attatattgc gcaagatacc agttccggtt
     2461 attcaggtgt tctatattca cgaagttcaa cgctccgcgc aaaaccgatc catgatggac
     2521 gattttgcga tgtcaggcga atatgctaaa tcatatgtct gaagcagtgt ccagcccata
     2581 tctggataaa ctgcagttct gacaggcata taaccagcag actctagtac gttctgtatc
     2641 ggtgatttgg cgaactcaca gataaagaac ggctaagcac taatatcagc aatacagtgg
     2701 cacagtattg tgatacgcga aataaaacat cagcacgtca gttcagagta aggtcgccat
     2761 atatcgggca tgagccagat acaaataatt gggacaccgt ctgaagtgat ttgctttatt
     2821 aaagtaactt gtgcttggtg tttatgttta tcaatttggg atcgcctgta aagtgcaaaa
     2881 tgcaatttcc atgaaagtgt cccctttaat ggggacacct gttcacattt tttcgttttc
     2941 tttagctgta ttgctaatca tgaaaacgaa cgttcgcatc agcacagcag agcgaagtgc
     3001 atcatatcct tccagattta agttcttcag gcttcgcttc aggtaggttc gtaccgtttc
     3061 gggagaaagc cgacagttct gagcaatggc gtcgtaagga acacccattg cgtaaaaaac
     3121 gcagacttta agctggtttg ttgatagctc gggaaacatg ccttccaaca gagtgatggc
     3181 cgggagtttc tgtatcagtt gaggtgacat agcaaaggtc tccgatgaca actaaaaacg
     3241 ctatcactga gttcctacgc tcattggtga tagcccagac gggggtagga ataccggcgt
     3301 catcggaaac cggccaggct aatgcctgcc ccgcctgagc taccatagat gacgacaaga
     3361 aatacaagtt cctgtcttta tggtatggca aaaaacgacg ccaaatgaag aaattaactt
     3421 ctgcgccgac gatgactacg cgggttccta cgcccgatca cagttttgcc tgtgaccagt
     3481 aaagggtact tagtataacg caccgagtca actaacagct taaaaaacat acaaaaaagc
     3541 gtcaaaactt aacgctccgc gccgaagcga tccaggcgtg atgcttctgc tttgtggcgt
     3601 tcgaacatgc tggtccatat gcacgaaacg gtgtctgacc ctcgtccaga caatccgcag
     3661 ttctgacgaa catatgaaca aatatgctct tgtagaggct gttgttacac aattggcggg
     3721 ttgtgtaaca a
//
LOCUS       DAFNBJ010000272         3727 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.273, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000272 DAFNBJ010000000
VERSION     DAFNBJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.273"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            29..304
                     /gene="ypdI"
                     /locus_tag="L2X49_004793"
     CDS             29..304
                     /gene="ypdI"
                     /locus_tag="L2X49_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HBN4799742.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(381..623)
                     /locus_tag="L2X49_004794"
     CDS             complement(381..623)
                     /locus_tag="L2X49_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBN4799743.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            976..1896
                     /gene="lpxP"
                     /locus_tag="L2X49_004795"
     CDS             976..1896
                     /gene="lpxP"
                     /locus_tag="L2X49_004795"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBN4799744.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2252..2323
                     /gene="ypdK"
                     /locus_tag="L2X49_004796"
     CDS             2252..2323
                     /gene="ypdK"
                     /locus_tag="L2X49_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBN4799745.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2388..3626)
                     /gene="alaC"
                     /locus_tag="L2X49_004797"
     CDS             complement(2388..3626)
                     /gene="alaC"
                     /locus_tag="L2X49_004797"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBN4799746.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          964 a          949 c          876 g          938 t
ORIGIN      
        1 cagtgaacca ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt
       61 tttattggtc tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag
      121 tgagaggagc tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa
      181 acttcagcca tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct
      241 cacagaagaa atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg
      301 atagcgtctg tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc
      361 aaatgagcca tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag
      421 ccacaagccg gggcaagaaa cataaatatc cccgtcacta gttcattacc gccagttaac
      481 cacatggtga agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc
      541 gccgatacca cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg
      601 agaaacatcc ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt
      661 cagtcacttt aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga
      721 aaacagcatg ttaaatgcaa gactgttgtg tacggaaaaa tatttacttt gcacgattaa
      781 taatcagtag ctgaaagcag tcagcgagtg ataaagtaca gataccgcat tcatgctcct
      841 cgtggttatg tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg
      901 cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat
      961 ctacagaagg taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc
     1021 tactggctca catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct
     1081 gttctctgct ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt
     1141 gaatctatcg cccgtaaaaa cctggaacta tgtttcccgc agcattctgc ggaagaacgc
     1201 gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg
     1261 gcatggttct ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac
     1321 cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg
     1381 ctggaactgg gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca
     1441 cataataatc agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg
     1501 atgatcggca gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta
     1561 tggtttgctc ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgct
     1621 gtggaaaatg tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc
     1681 atgttgaccg taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc
     1741 ccagagatgg aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt
     1801 atcgagaaag agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa
     1861 acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc
     1921 ctcaaatcgg ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt
     1981 cgctgaatat ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta
     2041 agttactaaa agtaaataag aaaatgtctc ttgtctcgtt tcagttttag gcgtacatta
     2101 gcgccgtctc gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg
     2161 cttttaaggt cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag
     2221 gcgtaacagt aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcatt
     2281 tatggtcatc gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg
     2341 aaaaacagga gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt
     2401 tcgtgaatat gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc
     2461 ccacgaatcg cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta
     2521 tcaccatagt cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc
     2581 ttcttggcaa attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca
     2641 tacatcgaag ccttcggcat ttcaaccatc cagcccgctt catgcagccc tttaaccaat
     2701 acatcacggc ggcgtttgta ctgttcggca atgtcgcgca cgcactgttg atcgccctcc
     2761 agcgccgcaa tcgccgccac ctgcaacggc gtaaaggtgc cgtaatcgtg atagctttta
     2821 atacgagcca gggcgctgac cagcgttttg ttgccaacca taaagccgat acgccagccc
     2881 gccatgttgt agcttttcga cagcgtaaag aactcgactg ccacatcgcg tgcacccggt
     2941 acctgcatga ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtcg
     3001 tggaccacca gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagc
     3061 tccacgcatt gcgcggttgg attcgacggg aagccgagga tcatcatctt cggtttcgga
     3121 taactttcac gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc
     3181 actgagcgta cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga
     3241 ttcggcacca gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc
     3301 tctttcgaac caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga
     3361 tcctgatacc agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa
     3421 ccatgcgtgt ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc
     3481 gcaccgtccg ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc
     3541 attttcagtt cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag
     3601 cgacgttcag ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt
     3661 ccgagcgacg ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt
     3721 ccagcgt
//
LOCUS       DAFNBJ010000273         3667 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.274, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000273 DAFNBJ010000000
VERSION     DAFNBJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.274"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            238..2520
                     /locus_tag="L2X49_004798"
     CDS             238..2520
                     /locus_tag="L2X49_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HBN4799747.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLMNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            2558..3238
                     /gene="ybiX"
                     /locus_tag="L2X49_004799"
     CDS             2558..3238
                     /gene="ybiX"
                     /locus_tag="L2X49_004799"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBN4799748.1"
                     /translation="MYDVPHSGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNN
                     QQVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAV
                     RSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHC
                     VTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHN
                     LLREWSEI"
     gene            3312..3578
                     /gene="ybiI"
                     /locus_tag="L2X49_004800"
     CDS             3312..3578
                     /gene="ybiI"
                     /locus_tag="L2X49_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBN4799749.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
BASE COUNT          936 a          989 c          920 g          822 t
ORIGIN      
        1 tggatgcagt tttcgtctgt tttgtaatca aaaaacatca aatatgaact caatgtaaat
       61 aaatgtattt ctttttcgcg caatgggtga tagaaaatcg cgccaagtga taatgcttat
      121 caaaattatt atcacttgca cgaacactat cacgggatta acagtggcat cgcatccgca
      181 gagatgcttt ctcgtggcag tgaaaatttc aacatataag aaaaagtcac ctgcaaaatg
      241 gaaaacaatc gcaatttccc tgccagacaa tttcattcgc tcacgttctt tgccggtctt
      301 tgtattggca tcacgcctgt ggctcaggca ctcgccgccg aagggcaaac taacgcggat
      361 gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg cgccacaaca atctgccgat
      421 ccgaaattct cgcgtccggt agcggatact acccgcacga tgacggtaat ttctgaacaa
      481 gtgattaaag atcagggcgc aaccaacctt accgacgcgc tcaaaaacgt ccccggcgtg
      541 ggtgcgtttt ttgcgggtga gaacggtaac tccaccactg gcgacgccat ttatatgcgt
      601 ggtgccgata cctctaacag tatttatatt gatggcattc gcgatatcgg cagcgtctcg
      661 cgcgacacct tcaataccga gcaggtcgaa gtgattaaag ggccgtccgg caccgactac
      721 gggcgcagcg caccgacagg ctcgatcaat atgatcagca agcagccgcg caatgattcc
      781 ggcattgacg cctccgccag tattggcagc gcctggttcc gccgcggcac gctggacgtc
      841 aatcaggtca ttggtgatac caccgcggtg cgcctgaatg taatgggcga aaaaacgcac
      901 gatgccggac gcgacaaagt caaaaatgag cgttacggcg tcgccccttc tgtcgctttt
      961 ggccttggta cagcgaatcg tttgtatctt aattatctgc atgtcaccca gcacaacacg
     1021 ccagacggcg gcattccgac catcggtttg ccgggctatt ctgccccatc tgcgggaacg
     1081 gcggccctga atcattccgg aaaagttgat actcataact tttacggcac ggattccgat
     1141 tacgacgatt cgaccaccga caccgccacc atgcgttttg agcacgacat caacgataac
     1201 accaccattc gcaatactac ccgttggtcg cgcgtaaagc aggattacct gatgacggcg
     1261 attatgggcg gggcgtcgaa tattactcag cccaccagcg atgtgaatag ctggacctgg
     1321 tcacgcacgg cgaataccaa agatgtgagt aataaaattc tcaccaacca gaccaacctg
     1381 acctcgacgt tctataccgg ttctatcggt catgatgtca gtaccggcgt ggaatttacc
     1441 cgtgaaacgc agactaacta cggcgttaat ccggtgacgt tacccgcggt aaatatttat
     1501 catcctgaca gcagcattca tcccggcggc ctgacgcgca acggcgcaaa cgccaatggt
     1561 cagacggata ccttcgcaat ttacgccttt gatacgctgc aaatcacccg tgattttgag
     1621 ctgaacggcg ggatccgtct ggataattat catactgaat atgacagtgc caccgcctgc
     1681 ggcggcagcg gacgcggtgc catcacctgc ccaactggtg tggcaaaagg ttctccggtc
     1741 accaccgtcg acaccgccaa gtcgggcaat ctgatgaact ggaaagccgg ggcgctgtat
     1801 cacctgacgg aaaacggcaa tgtctatatt aactatgccg tttcccagca gcctccgggc
     1861 ggcaacaact tcgcccttgc gcagtctggc agcggtaaca gtgccaaccg caccgatttt
     1921 aaaccgcaaa aagccaacac cagcgagatt ggcaccaaat ggcaggttct ggataaacgt
     1981 ctgttgctca ccgccgcgct gttccgtact gatatcgaaa atgaagttga gcaaaatgat
     2041 gacggaactt actcgcaata cggtaagaaa cgcgtcgaag gctatgagat atccgtggcc
     2101 gggaatatca ctcccgcgtg gcaggtgatt ggcggctata cccagcaaaa agcaaccatc
     2161 aaaaacggca aagatgttgc ccaggatggt tcctcatcgc tgccgtatac cccggagcac
     2221 gccttcacct tatggagcca atatcaggca accgacgata tctctgttgg cgcgggcgca
     2281 cgctatatcg gcagtatgca taaaggttca gacggcgcgg tgggaacgcc agcgtttacc
     2341 gaaggttact gggtcgccga tgccaaactg gggtatcgag ttaatcgcaa tctcgacttc
     2401 cagctaaacg tttacaacct gtttgatacc gattacgtcg cctcaatcaa caagagcggc
     2461 taccgttatc acccgggcga gccaagaacc ttcttgctca cagccaatat gcatttctga
     2521 ttcagatgtg gggcgcaggc cccacttttt ggagaaattg tatgatgtac cacattccgg
     2581 cgtgttatcg ccacaggacg tcgctcgttt tcgcgaacaa ctggaacaag ccgaatgggt
     2641 ggatggacgc gtcaccaccg gcgcacaagg tgcgcaagtt aagaacaatc aacaggtcga
     2701 cacccgcagc acgttatacg ccgccctgca aaatgaggtg ctgaacgcgg ttaaccaaca
     2761 tgctttattc tttgccgcgg ccttgccgcg taccctttcc acgccgctgt ttaatcgcta
     2821 tcagaacaat gaaacctatg gtttccatgt ggatggcgca gtacgcagcc atccacaaaa
     2881 cggctggatg cgtactgacc tttctgccac gctgttttta agcgatccac aaagctacga
     2941 cggcggcgaa ctggtcgtta atgacacctt cggacaacat cgggtaaaac tcccggcagg
     3001 cgatctcgtg ttgtatccct ccagcagcct gcattgcgtg acacccgtaa cccgcggcgt
     3061 acgagtggca tcatttatgt ggatccagtc gatgatccgc gatgataaaa agcgcgccat
     3121 gctgtttgaa ctggacaaca atattcagtc gctgaaaagc cgctacggcg aaagtgaaga
     3181 gatcctgtcg ctgcttaatc tttatcataa tctgctgcgg gaatggtcgg agatctgatg
     3241 cttgaactgc ccgagttcgt ccctggatta gctacactta actgtacaag tattgatatg
     3301 gggaggtcga tatggcatcc ggttgggcta acgatgacgc cgtcaacgaa cagatcaaca
     3361 gtacaattga agatgcgatt gcccgcgctc ggggtgaaat tccgcgcggc gaaagcctgg
     3421 atgaatgtga agagtgcggt gcccccatcc cgcaggcccg tcgggaagcc attcctggcg
     3481 tgcgcttatg tattcattgt cagcaggaga aagatttaca aaaaccagct tatacaggat
     3541 ataatcgcag aggttcgaaa gacagccagt tacgttaact acaactggcg agatgcatag
     3601 cgagtataat ttgtattttg cgtggcattc cgtgccttta acgctgctgt gactgccacg
     3661 cggtata
//
LOCUS       DAFNBJ010000274         3636 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.275, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000274 DAFNBJ010000000
VERSION     DAFNBJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.275"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(135..1292)
                     /gene="intS"
                     /locus_tag="L2X49_004801"
     CDS             complement(135..1292)
                     /gene="intS"
                     /locus_tag="L2X49_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBN4799750.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASPNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANSGSVRGIYNHAQYLDKRREMMQWWADWLDGKVD"
     gene            complement(1454..1528)
                     /locus_tag="L2X49_004802"
     tRNA            complement(1454..1528)
                     /locus_tag="L2X49_004802"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1493..1495),aa:Arg,seq:cct)
     gene            complement(1604..2536)
                     /locus_tag="L2X49_004803"
     CDS             complement(1604..2536)
                     /locus_tag="L2X49_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBN4799751.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2831..3586
                     /gene="mlaA"
                     /locus_tag="L2X49_004804"
     CDS             2831..3586
                     /gene="mlaA"
                     /locus_tag="L2X49_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBN4799752.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          860 a          873 c          866 g         1037 t
ORIGIN      
        1 ccctgatata atataaatcg ccaaggatgg ctcattcaaa taagaatctt tagtaatcag
       61 attgttaatt aaagatggtt tgttggtttt tatgtttatt aaaaaaggtt atatcaacgc
      121 gctgttattg ttctttaatc aaccttccca tcaagccagt ctgcccacca ctgcatcatt
      181 tctctgcgtt tatcgagata ctgagcatgg ttgtaaatcc cgcgtacaga tccgctgttg
      241 gcatgtgcca gttgcacttc aatagcgtca gcaggccatt cgtgctcgtt cataattgtg
      301 ctgaattcat gcctgaatcc gtgaccgctt tccagaccct catagccgat ttgtttgatc
      361 acaagcagca ccgcgttctc gcagattggc ttcttcttat cgttgcgccc ggcaaaaaca
      421 aactctgata ctggtttagt gatggagctt agcgtagtga gaagttcaac cacctggtcc
      481 gacatcggga caacatgaat cttgcgtccc ttcatcacac tggcgtcgat agtgataatc
      541 ctgttttcaa aatcgacgtt cttccattgc atggagcgaa gctctttcgt tcttagggct
      601 gtgtagcgta aaactttggt cgcaatgagc gatacgatac ttcctgaaaa tgttgccagt
      661 gctttgttga atgccgggat ctggtctgca ggaagaaacg ggaagttctt cttgcggtat
      721 cccttcatgg cgtcagcaag gtcaggtgct gggttatatt tagcccttcc tgtgacaata
      781 gcgtaacgga aaacctctcc acatcttctg cgggctttgt tggctcgctc cattgcaccg
      841 cgatcttcaa atctgcggat tacttccagc agttgcatcg gctcaatatc ctgaatctca
      901 aggccgccga tgatgggtaa aatgtcgtca tcaaacattt tggcaagttc agtcgcatac
      961 cctactgacc atacttgctt cttgtgctcg taccattcct tgtaaatggc gctaaaggaa
     1021 ttgttattag gcgaagcctt tttcgctttt accggatcga tgccaaccga gatgtctttc
     1081 ctcgcggtcc atgctttatc ccttgcctcc tgcaaagtca taagcggata ttttccgacg
     1141 gtcaggattt tctccttacc gtcaatcttg tagcgaagct gccatacctt tttccctgat
     1201 acagggacat aaaggtacag gccattacca tcgagtaggc ggtatggttt ttctttcggc
     1261 tttgctgctt caatctgctt aacagtgagc atgggtaaaa atccggtggg taaaattatt
     1321 ttatccactt tttacccgtc atggtgtgcg gctgtcaacg atctgacgcg aaccattacg
     1381 aactgtgaat ctacggaagg cttgatattc aggggatttt gcggactggt acggatggga
     1441 gcgaactgat aaatggtgtc ccctgcagga atcgaacctg caattagccc ttaggagggg
     1501 ctcgttatat ccatttaact aagaggacaa tgcggcatga gtatacccgc taatggagtg
     1561 cggggtaagt acgctgccgc tcgattgctt aaaccctcgc catttatgcc gggtttttat
     1621 catttttctt aatgttttcc gcacgttctg ctttttggcg tgcttctgct ttacgcttgt
     1681 tgctcatgtc gttacgaatc tgtgcatgac tcattaacgc gaagataaag gtgccgccgc
     1741 agatgttccc cgctaaagta ggtagtgcga agggccagat gaaatcgctc cagtgcagcg
     1801 taccgttaaa caccagatag aggatttcaa cagaaccgac cacgatatgg gtggtgtcac
     1861 ccagggcaat aagccaggtc atcaatataa tcaccacaat ctttgccgca cctgctgcag
     1921 gaaacatcca taccatagtg gcgatcagcc agccggaaat gatcgcgttg gcaaacatct
     1981 cgctgggggt gttcttcatc acatccatgc cgattttgac aaatgcatcg cgagtttctt
     2041 cattgaagat aggcatatat tcaaatgccc atgcagcaat acctgtcccg agaatattac
     2101 ccagcagcac gacgccccat aatcgcataa gtaagccgac gttgctcatt gtcggttttt
     2161 gcatgacggg tagtaccgca gtcacggtgt tttcggtaaa taattgctgg cgggccataa
     2221 tgacgataat aaaaccaaag gtataaccga gattctccag taagaagctg cccggcacac
     2281 cttccagttc gacatgaaat atcccttttg ccagtaacga agcgcccatc gacagacccg
     2341 ccgcaatggc tgaccacagt agcgccattg cgtcgcgttc cagctctttt tcaccatcct
     2401 ggcggatatg ctcatgaatt gccatcgccc gggaggggag tcggtcttca tctatttcta
     2461 tttttttgcc gcgctctttt tcttcgctct caacttcaat ttcgtcgctg tgttgatcaa
     2521 ttttgtcgtt gtccatggtc tcttctcttg aattagcacg tatagctaaa gcgtagcggc
     2581 ttttttgctc gcaactggcg ggagttactc tgaaaatgta gaaaaggctg cgtttgcctt
     2641 tttctgtttc tatagaatca agtagcctac agggcggcga ttaccaggct atgatcaaat
     2701 cagcaaatca gggcgtctgg acatcagttg acgtgctgtt acaatcgccc acacctaaac
     2761 aggcggatac ggtatcgttc cgtcatggat ggcaaactgc ataagccata aaaaaaacag
     2821 ggagacattt atgaagcttc gcctgtcggc gcttgctctg ggaactacgc ttctggtggg
     2881 gtgtgcgagt tccggtacag atcagcaagg gcgttctgac ccgttagaag ggttcaaccg
     2941 caccatgtac aacttcaact tcaacgtatt agacccgtat attgttcgac cggtcgctgt
     3001 cgcctggcgt gattatgttc cgcaaccggc gcgtaatggt ttgagcaact ttactggcaa
     3061 ccttgaagaa cctgcggtga tggttaacta cttcttgcag ggcgaccctt atcaggggat
     3121 ggtccacttt acccgctttt tcctgaacac cattttgggg atgggcggtt ttattgatgt
     3181 tgcagggatg gcgaacccga aactgcaacg gactgaacct caccgcttcg gtagtacgct
     3241 tggtcattat ggcgtgggtt atgggcctta cgtccagtta ccgttctacg gtagcttcac
     3301 gctgcgtgat gacggtggtg atatggcgga tggtctttac ccggttcttt cctggctgac
     3361 ctggccgatg tctgtgggta aatggacgct tgaagggata gaaactcgtg cgcagttgtt
     3421 ggattccgat ggtctgctgc gtcagtcgtc cgatccttat attatggtgc gcgaagcgta
     3481 cttccagcgt catgatttca tcgctaatgg cggcgaactc aaaccgcagg aaaatccgaa
     3541 cgcacaagcg attcaggatg atttaaaaga tattgattct gaataagaaa caaataaaaa
     3601 aggtgagtcg caaaactcac cttttttgtt gttatc
//
LOCUS       DAFNBJ010000275         3586 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.276, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000275 DAFNBJ010000000
VERSION     DAFNBJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.276"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(236..988)
                     /gene="yecC"
                     /locus_tag="L2X49_004805"
     CDS             complement(236..988)
                     /gene="yecC"
                     /locus_tag="L2X49_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBN4799753.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATERARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(985..1653)
                     /gene="tcyL"
                     /locus_tag="L2X49_004806"
     CDS             complement(985..1653)
                     /gene="tcyL"
                     /locus_tag="L2X49_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBN4799754.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1668..2654)
                     /gene="dcyD"
                     /locus_tag="L2X49_004807"
     CDS             complement(1668..2654)
                     /gene="dcyD"
                     /locus_tag="L2X49_004807"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBN4799755.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKGEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2759..3559)
                     /gene="tcyJ"
                     /locus_tag="L2X49_004808"
     CDS             complement(2759..3559)
                     /gene="tcyJ"
                     /locus_tag="L2X49_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN4799756.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTLKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          870 a         1004 c          840 g          872 t
ORIGIN      
        1 ctgcatagta aaccgcaacg cccctgagag tgagtatcat ttataatgat aattcaaact
       61 atcacataaa taagctttat atataattta tattattcag gcaatgaatt acttttgcaa
      121 gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca gttttatcac
      181 gcttgaaatg gcgtcccgcc ccgacaggtg aatcgtcggg gctgattatt tcttattatt
      241 gcagcagaaa cttctcaagg aactggcggg tgcgaggctg ctggggatcg gcaaataacg
      301 cttttgctgc cccctgctcg actatccgcc cctggtccat aaaaatcgcc cggtccgcaa
      361 catcacgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca
      421 gctgacggat ggtgttcagg acttcgccca ccagctctgg atccagcgcc gacgttggct
      481 cgtcaaacaa aatcacctca ggacgcattg ccagcgcacg tgcaatcgca acacgttgct
      541 gttgaccgcc agacaaacga cgtggatagc tggtttcttt accggccagc ccaactttcg
      601 ccaacagctc gcgagcgcgc tccgtggcct cttctttcgg ttcgcctttg acgatcaccg
      661 gcccttcaat aatgttttcc agtaccgtac gatgcggaaa caaattaaag ttctggaaga
      721 caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt aatgaacgtg
      781 cagtatcaat agtgatatcg ccaacggtga tagttcccgc ttccggttgt tccagcagat
      841 ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataatt gccaccactt
      901 cgccaggctt tacctcaagg tcgataccgt gcagcaccgt ctgaccgtga aattttttca
      961 ccaggttctt aacttcaatg gcactcattt tggttctctc tcctggcgat taagttgatt
     1021 ctcaaaatgg ttctgcaacg tcgataacac agtcgccatg atccagtaga tgagcgaagc
     1081 cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg cctgacggaa
     1141 cagctccggc acctggattg tcgcggccag cgacgtatcc tttaccaggc tgataaagct
     1201 gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatagt
     1261 ctgccatggc gtcataccaa tactggcagc ggcttcccac tgacctttat caatagaaga
     1321 aatggcggcg cgcagcgttt cggcagcata ggcggcagtg ttgagcgaca gaccaatcat
     1381 tgctgacgga atgggatcta attcgatacc aaactgcggc aggccgtaat agatcataaa
     1441 cagttgtgcg atgagcggcg taccacgaaa aatagagata taaaaacgcg ccagccagcg
     1501 cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagca gtaagccaaa
     1561 aaacatgccg ccgatactga gttgcagcgt atacccagcc cctttcagca ggaacggcaa
     1621 ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta aacgtgggga
     1681 tgataggcga acagcgcagg cgcaccgccg gtatgaataa acagaatcgg cccttcacct
     1741 ttgaagcgtt tctgactgat accgtcaatc agccccgcca tcgcttttcc ggtatacaca
     1801 ggatcaagca gaatgccttc cagccgcgcc agcaatttca ctgcttccat gccttcgtcg
     1861 ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc cgctgatgcg
     1921 gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg caattgatcg
     1981 gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc
     2041 ccaacagcca atccggcgtg agttccggca ctgcccgatg cgaccaccac cgacgaaata
     2101 ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac tctccacata acccagcgcg
     2161 cccagagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttcgact
     2221 cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcgtcgca catttcaatc
     2281 tgggtattga acagatccag caacaaacga ttgccgttgg ttaaatagtt ttctgcggtt
     2341 gtgccaatag gattttccag cagcgccacg cagtgcagac cgagtttcgc cgcaactgcg
     2401 gcagtctggc gcacatggtt agactggatc gccccggcag taatcagcgt atcggcacct
     2461 tcgcgcagag catctgctgc gagaaattcc agcttacgta atttattgcc gcccattgcc
     2521 atgggggtga cgtcatcccg tttgatgaaa atttcccgtc caagataatc agaaaagcgc
     2581 ggcagatatt cgagcggcgt tggcgcgccg ataaactcca accgtggaaa acgggttaaa
     2641 ttatgcagtg gcataacagc ctccgatgtg tgttgttgtg attttcttat tatgcacgct
     2701 gaaaacgcgt aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat tatgctgatt
     2761 atttggtcac atcagcacca aaccattttt cggaaagagc ttgcagagtg ccgtcttttt
     2821 gcatttccgc aattgcatca ttcaccgctt tcagcaggtc ctcatttcct ttacgcagcg
     2881 ccacgccaga ctcctgacgg gagaatgctt caccggttac tgccagcgta tcgttggttt
     2941 tcttcaccag atccagcgcc gccagacgat caacgaggat cgcatcgata cgccctacgc
     3001 gcagatcctg atatttggtc gggtcatcat cataggtacg cacatcgaca ccctgaacat
     3061 tctgccgcag ccactcttca tagttggtgc ccagaccgac acccactttt ttgcctttca
     3121 gatcagcagc cgttttaagg gtgccttcgt tacctttttt caccagcgcc tgaataccgg
     3181 aaatggtgta aggggtagag aaatcgtatt ttttcttgcg ctcgtcagaa atggtgacct
     3241 gattaatcac cacatcaatg cgtttagagt ccagcgacgc cagcataccg tcccatttgg
     3301 tcggttttag tgacgcctca acgccaagat gttttgccag ctgttgggca aattccactt
     3361 caaaaccggt taatttgccg tcatccccct gaaaactgaa cggcggataa gttccttcca
     3421 gccctaccag tagcgtaccg cgctctttga ctttattaag cagaccttca tctgcaaaac
     3481 ttttaacgct catgcccgca accagcgcca cggccatcac acccatcaat gcctgacgtc
     3541 ccagatgtgc taatttcata ttcaccccga atgttgttat gtctgt
//
LOCUS       DAFNBJ010000276         3563 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.277, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000276 DAFNBJ010000000
VERSION     DAFNBJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.277"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(1..2248)
                     /locus_tag="L2X49_004809"
                     /pseudo
     CDS             complement(1..2248)
                     /locus_tag="L2X49_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
     gene            2262..2594
                     /locus_tag="L2X49_004810"
     CDS             2262..2594
                     /locus_tag="L2X49_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HBN4799757.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSTFAV"
     gene            complement(2641..3516)
                     /locus_tag="L2X49_004811"
     CDS             complement(2641..3516)
                     /locus_tag="L2X49_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-15"
                     /protein_id="HBN4799758.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
BASE COUNT          820 a          973 c          918 g          852 t
ORIGIN      
        1 ggactatttt tcggggatct gattgccctc tggcaatatc attcagcacg ccatagtcgg
       61 catcatggtc cattcgccag aaaaccgaag caccggcatc agccaggcgt ggagaaaact
      121 ggtatcccag cagccagaaa aggccaaaga caagttcgct ggcacctgct gtatcggtca
      181 taatttcggt tggattcagc ccggtctcct gttccagaag accttccagc acaaagatag
      241 agtccctcag cgtccccggt ataacgatgc catgaaagcc ggaatactga tcggacacaa
      301 agttgtacca ggtgatccct ctgttattac caaagtattt gcggttcggt ccggcattga
      361 ttgttctgac tggcgtaaca aagcgcattc catctgcaga tgccacttct cctccacccc
      421 atatctgtgc cagtggcagc gttgcctgaa aatcaaccag tctggcatta gcgctggtga
      481 tagtttcagc ccgcagatag ttcgcttttg tccagttcag ccggtgtcgg gtcagtgcag
      541 gaacatttga tctgatcagt ggttccagac cgatattgca ggcttcagcc atcagcacgg
      601 cgctgatgct gacgggcaga tcatcaactc tggcactggc ttcactagca tggaaaaact
      661 catcagcaaa tccggtatgg gcgttaattt cgagcagcaa ctccgttaaa tccaccggag
      721 ggagtagatc actgatcatt ttgctcagtc gtttcagact gtccggctca tcaagactgg
      781 cgaggggaga aattgtcaac cggggcttcg ggccagaaac atcgagttcg acagcctcat
      841 tttcgcaaag acgtgcagca acctgtctgt aacgactatc aagctgatga cccagagatt
      901 ttattgcttc ctgcgggtct gtcgggtgcc ccaaagaacg ataaacctta atccggtttg
      961 cctgccagtc agcaccctgt agtaatcttg cacgaggatc tccccaccgg ttactgccgg
     1021 taacgtagac atccctccgc ctcagactat cctgcagttt actgagaaag cagagcgtgt
     1081 atcccctgcg ggtgatatgt ttttccttgt taatcaccag ccgtttccat gaccgactga
     1141 taatttccgt tggtgcgtcg tcaaaaaact gccgccgtga gctgaactcc cggctgaggt
     1201 agtcacaggc attcagagtg gtaaccccgg caggtgcgga tgaaaattta acggtattca
     1261 gcagatgggg caggaaacga cgaacgcgcc cgtactgctc caccatttct tcatgaaaat
     1321 tatcgtctga gggccgggca atttcacgga caagcgtgat gatttcagcc agcttttgcc
     1381 ttgggatgta gctgaacacc tcagcacgaa tcgattcgtc cggtgtttct tctttcagca
     1441 ggtacgaaca tgcgctggcg agcgccaatg cagatttatc cagatccttc agcgagcgga
     1501 gccgtttttt ctgcccaatc tttctggcgt cacggatgat aacggccagc atggcgtcca
     1561 gaacgtccaa tgcatcatcc agcgccagcg tttcccatgc aaggacaaag gcaaccagaa
     1621 ccgccatcct tttctgcggt gacatcctgg caatattgaa caccgaagtc ataccagcat
     1681 aacgtgcgag atttttcagg cgcacagccg ggagtgtact caggttttca gcatgcaggc
     1741 caaaatcgtt cagagttttc cagcgttcaa ttgcttcatt aaacgccgga ccactgatgg
     1801 tcacagggcc ctttttcagt gattccagta aagacaggcg gctgcaatca gttggcccca
     1861 gcagcatctc cagctgtgaa cgctgttcgg ctgacggtat cagtgccagt ttgttccaca
     1921 ggcgcaacgt cgccttttcc cttacctctg aaatcaaccg ggtcagcgta gtggctccgg
     1981 ggagaataat acgatgttgc ataagccacc ctgtcgccag atcgaaaagc aggccaggac
     2041 gttcgttgct tatccagctc cgggtatata aaagacgggt aaggcgaaat gtccagggcc
     2101 aggcaaattc acgatactga tagtgctgac gtatcagcgc tgcatgctca cggcgggtat
     2161 tttccctctg accgtattct gcaagaacgg tgatatcacg aatcccgagc tgtctggcgg
     2221 taaaatgccg gacgccggaa ggaatatgac ctgggaccta cgtgcgcccg caccgacacc
     2281 ctcacacctt cgagctactg ttgccattaa ggggtcgttt cgtggtgctg aattttgacg
     2341 atcggggtac cgtcacccat cgggcgatat tgggggaaac ctgtacggtg ctggagatgg
     2401 ccgcaggaac ctggcatgcc gtgctgtcgc tggataccgg tggcataatt tttgaagtaa
     2461 aacacggtgg ctatcaaccc gtggctgccg atgactatgc gcactgggct ccagcggaag
     2521 gagaaccagg aaccacggag cttatggcct ggtatgcgca agcgcaggtg ggcgacagca
     2581 cttttgccgt ctaaggcgat aaacaaaaac ggaatgagtt tccccattcc gtttccgcta
     2641 ttacaaaccg tcggtgacga ttttagccgc cgacgctaat acatcgcgac ggctttctgc
     2701 cttaggttga ggctgggtga agtaagtgac cagaatcagc ggcgcacgat cttttggcca
     2761 gatcaccgcg atatcgttgg tggtgccata gccaccgctg ccggttttat cccccacaac
     2821 ccaggaagca ggcagtccag cctgaatgct cgctgcaccg gtggtattgc ctttcatcca
     2881 tgtcaccagc tgcgcccgtt ggctgtcgcc caatgcttta cccagcgtca gattccgcag
     2941 agtttgcgcc attgcccgag gtgaagtggt atcacgcgga tcgcccggaa tggcggtgtt
     3001 taacgtcggc tcggtacggt cgagacggaa cgtttcgtct cccagctgtc gggcgaacgc
     3061 ggtgacgcta gccgggccgc caacgtgagc aatcagctta ttcatcgcca cgttatcgct
     3121 gtactgtagc gcggccgcgc taagctcagc cagtgacatc gtcccattga cgtgcttttc
     3181 cgcaatcgga ttatagttaa caaggtcaga ttttttgatc tcaactcgct gatttaacag
     3241 attcggttcg ctttcacttt tcttcagcac cgcggccgcg gccatcactt tactggtgct
     3301 gcacatcgca aagcgctcat cagcacgata aagtatttgc gaattatctg ctgtgttaat
     3361 caatgccaca cccagtctgc ctcccgactg ccgctctaat tcggcaagtt tttgctgtac
     3421 gtccgccgtt tgcgcataca gcggcacact tcctaacaac agcgtgacgg ttgccgtcgc
     3481 catcagcgtg aactggcgca gtgatttttt aaccatggga ttccttattc tggaagatac
     3541 gaaataacaa caacatgaat agt
//
LOCUS       DAFNBJ010000277         3543 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.278, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000277 DAFNBJ010000000
VERSION     DAFNBJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.278"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..208)
                     /locus_tag="L2X49_004812"
     CDS             complement(<1..208)
                     /locus_tag="L2X49_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HBN4799759.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDM"
     gene            372..929
                     /locus_tag="L2X49_004813"
     CDS             372..929
                     /locus_tag="L2X49_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBN4799760.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            1163..1717
                     /locus_tag="L2X49_004814"
     CDS             1163..1717
                     /locus_tag="L2X49_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ib'"
                     /protein_id="HBN4799761.1"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLA
                     DVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGWWEEETDPGVRGIDQS
                     LANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTV
                     TTPDGPAVYMVQTRQAFERTRSVA"
     gene            1787..2575
                     /gene="aadA1"
                     /locus_tag="L2X49_004815"
     CDS             1787..2575
                     /gene="aadA1"
                     /locus_tag="L2X49_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBN4799762.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            2635..3459
                     /locus_tag="L2X49_004816"
     CDS             2635..3459
                     /locus_tag="L2X49_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-9"
                     /protein_id="HBN4799763.1"
                     /translation="MKKILLLHMLVFVSATLPISSVASDEVETLKCTIIADAITGNTL
                     YETGECARRVSPCSSFKLPLAIMGFDSGILQSPKSPTWELKPEYNPSPRDRTYKQVYP
                     ALWQSDSVVWFSQQLTSRLGVDRFTEYVKKFEYGNQDVSGDSGKHNGLTQSWLMSSLT
                     ISPKEQIQFLLRFVAHKLPVSEAAYDMAYATIPQYQAAEGWAVHGKSGSGWLRDNNGK
                     INESRPQGWFVGWAEKNGRQVVFARLEIGKEKSDIPGGSKAREDILVELPVLMGNK"
BASE COUNT          930 a          835 c          945 g          833 t
ORIGIN      
        1 tcatatcggt gaggaaggtg cccagaaaac ggacacatcc aatttgcagg gcaatgccca
       61 gacggttgtg atcacctctg ctttttccga taaattcctt gtctgcttca tcaaggtgaa
      121 aatatcgtgc cagctgaagc tcatccggtt caccggtgaa tctgccatag ctttcagtct
      181 gctcagtggt cagaaagtca acgggcatat cggcctccct gcctgacggg catttagtaa
      241 catttttcca accgtacgaa atgttataaa ttatcggaca tcgtaaaact gttacattaa
      301 tatgtctatt aaatcgtaaa tttgtaataa tagacatgag ttgtccgata ttcgatttaa
      361 ggtacatttt tatgcgactt tttggttacg ctcgggtctc aaccagtcag cagtctcttg
      421 atcttcaggt cagagcactc aaagacgcag gtgtgaaagc aaaccgtata tttaccgata
      481 aggcatccgg cagttcaaca gaccgggaag ggctggattt gctgaggatg aaggtggagg
      541 aaggtgatgt cattctggtt aagaagctcg accgtcttgg ccgcgacact gccgatatga
      601 tccaactgat aaaggaattt gacgctcagg gcgtggcagt ccggttcatt gatgacggga
      661 tcagtaccga cggtgatatg gggcaaatgg tggtcaccat cctgtcggct gtggcacagg
      721 ctgaacgccg gaggatccta gaacgcacga atgagggccg acaggaagca aagctgaaag
      781 gaatcaaatt tggccgcagg cgtaccgtgg acaggaacgt cgtgctgacg cttcatcaga
      841 agggcactgg tgcaacggaa attgctcatc agctcagtat tgcccgctcc acggtttata
      901 aaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      961 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1021 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1081 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc aaacaaagtt
     1141 aggcatcaca aagtacagca tcgtgaccaa cagcaacgat tccgtcacac tgcgcctcat
     1201 gactgagcat gaccttgcga tgctctatga gtggctaaat cgatctcata tcgtcgagtg
     1261 gtggggcgga gaagaagcac gcccgacact tgctgacgta caggaacagt acttgccaag
     1321 cgttttagcg caagagtccg tcactccata cattgcaatg ctgaatggag agccgattgg
     1381 gtatgcccag tcgtacgttg ctcttggaag cggggacgga tggtgggaag aagaaaccga
     1441 tccaggagta cgcggaatag accagtcact ggcgaatgca tcacaactgg gcaaaggctt
     1501 gggaaccaag ctggttcgag ctctggttga gttgctgttc aatgatcccg aggtcaccaa
     1561 gatccaaacg gacccgtcgc cgagcaactt gcgagcgatc cgatgctacg agaaagcggg
     1621 gtttgagagg caaggtaccg taaccacccc agatggtcca gccgtgtaca tggttcaaac
     1681 acgccaggca ttcgagcgaa cacgcagtgt tgcctaaccc ttccatcgag ggggacgtcc
     1741 aagggctggc gcccttggcc gcccctcatg tcaaacgtta aacatcatga gggaagcggt
     1801 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     1861 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     1921 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     1981 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     2041 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     2101 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     2161 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     2221 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     2281 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     2341 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     2401 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     2461 tgaagctagg caggcttatc ttggacaaga agatcgcttg gcctcgcgcg cagatcagtt
     2521 ggaagaattt gttcactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     2581 aaaacaaagt tatgcaccta ttaagcgcac agcggagcaa tgaaggatac cttgatgaaa
     2641 aaaattttgc tgctgcatat gttggtgttc gtttccgcca ctctcccaat cagttccgtg
     2701 gcttctgatg aggttgaaac gcttaaatgc accatcatcg cagacgccat taccggaaat
     2761 accttatatg agaccggaga atgtgcccgt cgtgtgtctc cgtgctcgtc ttttaaactt
     2821 ccattggcaa tcatggggtt tgatagtgga atcttgcagt cgccaaaatc acctacgtgg
     2881 gaattgaagc cggaatacaa cccgtctccg agagatcgca catacaaaca agtctatccg
     2941 gcgctatggc aaagcgactc tgttgtctgg ttctcgcagc aattaacaag ccgtctggga
     3001 gttgatcggt tcacggaata cgtaaagaaa tttgagtacg gtaatcaaga tgtttccggt
     3061 gactcgggga agcataacgg cttgacccag tcatggctga tgtcgtcgct caccatatct
     3121 cccaaggagc aaattcagtt tcttctacgc tttgtcgcgc ataagctgcc tgtatccgaa
     3181 gcggcttatg acatggcgta tgccacaatc ccgcagtacc aggcagccga aggatgggct
     3241 gtacatggaa aaagcggcag cggctggctt cgggacaata acggcaagat aaatgaaagt
     3301 cgtccgcagg gctggttcgt gggctgggct gaaaaaaacg gacggcaagt tgttttcgcc
     3361 cgattggaaa taggaaagga aaagtccgat attcccggcg ggtctaaagc acgagaggat
     3421 attctcgtgg aattacccgt gttgatgggt aacaaatgat atgtggcgtc atcgagagca
     3481 gatgcataac cctgcgctcg agcggacctc gcgcataaag ccgcgcgagt ccgctcacct
     3541 tga
//
LOCUS       DAFNBJ010000278         3511 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.279, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000278 DAFNBJ010000000
VERSION     DAFNBJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.279"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            21..653
                     /locus_tag="L2X49_004817"
     CDS             21..653
                     /locus_tag="L2X49_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4799764.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGDKINELFIRSHSVQH"
     gene            987..1796
                     /locus_tag="L2X49_004818"
     CDS             987..1796
                     /locus_tag="L2X49_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN4799765.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTDDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            1789..2271
                     /locus_tag="L2X49_004819"
     CDS             1789..2271
                     /locus_tag="L2X49_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799766.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(2304..2384)
                     /locus_tag="L2X49_004820"
     CDS             complement(2304..2384)
                     /locus_tag="L2X49_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799767.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(2420..2845)
                     /locus_tag="L2X49_004821"
     CDS             complement(2420..2845)
                     /locus_tag="L2X49_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799768.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAENVLTQTT
                     SWTLKSRYVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            3039..3476
                     /locus_tag="L2X49_004822"
     CDS             3039..3476
                     /locus_tag="L2X49_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799769.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYKFSITDDLMSMAVNELIKLT
                     KIENNVDLNKFTTICTDVLSPRVTRHNKEKKQTTFYPSQKSPF"
BASE COUNT         1229 a          615 c          605 g         1062 t
ORIGIN      
        1 cggataggga ggttcttaac atggggcttt gtagtcgtta taaaagtctt acatgcaata
       61 gttgctcaat gcattgccaa ataatgccag aagagtcacc gcgtttgcag tactgcgcaa
      121 actcgtgttt ttgtatgtgg cccgaagaaa gttcatattt taatcgtggt gtggtagaag
      181 gtatcttaac aaaaaaccac aatgcgagat taagcggata tatttttgtc gatttttcag
      241 taagtttttt acgtctattc cttgaaaaag actggattga ctatcttgca agtactgaca
      301 tggggattgt tttagtcagc gaccgcaata tgcaatcgtt agctaattac tggcgaaaac
      361 acaactcagc aatatctgct gtcatatata atgatgatgg tcttgatgtt gccaatgaaa
      421 agatcagaca actatttatt ggtcgttatt tatcatttac cagaggtaac acattaactc
      481 agatggaatt caccataatg gggtatatgg tttctggtta taatccatat cagattgctg
      541 aagttctgga tatggatatc cgtagcatct acgcgtacaa gcaacgaatc gaaaagagaa
      601 tgggtgataa aataaacgaa ttatttattc gttcacattc ggtccaacat tgatactcaa
      661 cgaccaggca gaatcatact ctgtttatac gggcatttat tgaaatgtcc gtattttata
      721 aataggccgt cccctcaaga gtaaacacca tagtatttaa aaccatcaca cgaaaaattc
      781 agaagcatta cgaaatatga atttacaaac aatagtggca taaatgttaa ccatgttaat
      841 ttacgtaaag ttttacgttg caacattaaa gcctcatttc aatcatcatg ataaatataa
      901 aattaatata tatttatgcc gtaaatacca atatacttag caaactatgt gatctccatt
      961 tcgattgatt tagtgtttat tgacgtatgt actggattat taacgataat atcgagttct
     1021 ggcctgagca ccgaaaatta atatcggtac ataacgccga tcttaacgtc gttctgacaa
     1081 cgccagccag tcgatgttta tcacttctac ttgaagcttt tcctgatgtg gttgcacaac
     1141 aagatttttt caccagagtc tgggaagaag aaggtatgcg tgtgcctact aacacgttat
     1201 atcagaacat atccattatc agacgcggat ttcgcgctgt tggtgatact acccactcgc
     1261 taattgcaac cgtgccgaga agaggattca agatccataa tgacattaac atacaaaatc
     1321 atgtaataaa ctcgtcaaca gacgcacata cacacaatgc cccacctgcc ataaaagtta
     1381 atgcgggata caaagagagc attggtggcg caaagaattt caataacaaa atcctcaaac
     1441 atataaaaag tcatctaatt atgttgagcg catttgtcat aggcgcatat tctgcatatt
     1501 ggctatggaa taataatcaa ccgaagccat ttttcaagga ttacaagact gttgcggaaa
     1561 ttaatggctg ccattttaat gtaacagacg atacaattga tgggttgaaa gagttcgata
     1621 aatataaaac acggatactg gattcgggga ttaactgtaa aaaacatccg tggttatact
     1681 tccctcttgc aaaatcctca ccggggatga ttgttatggc atgcaataaa aactataacc
     1741 aacatgaagt ggcagactgt ttaaccctgt cttaccgtga ggttaatcgt gattgattat
     1801 aaaaaaaact tattatttat tttagttttt atcagtgggt ttattctctt cactgtctat
     1861 tcttacactg cagaaaaaat gatatataac gaaacatgca ctgcaaattg ggtaattttt
     1921 aatgaccagg gacgagcaaa tcttaccatc gactttatgt ataacaaaaa aaataaaaca
     1981 ggaactgttg cgttgagtgg aacctggcaa caaggtaaca gagaaagtaa atcaatacgg
     2041 cgaaatattg aatacacatg gattgaaaac tatgacacag cccatttaac atcaaagaaa
     2101 gttaataagt ttgagattat ggatcaagtt gatgatgata gacttgcaca acttattcct
     2161 gatttttatg tctttccaga aaaaagtgta agctataata tactaaagca aggtaagcat
     2221 gcttttattt tgagcattgg taacagagca ataatgcatt gtgcaaggta aactaaaata
     2281 gataacaaaa agatggaacc tcgttaatca taaaacaata aaattaaata ttctcgcagt
     2341 atatggcagt ctaaagcatc aaagttttga tcaacatctt tcattttaga catctccttg
     2401 caatgtaaaa tatcatgtat caacaacatc tggtgcaaat ccatgagtcg aactcgacat
     2461 tcatcttagc tcgattatta ttccttcgta cagtcgatgt aaacaataca gaaagaggat
     2521 tattaagaac cagtttactc agtgaatcaa tagaggaaag gactaacgtt tctttaaaag
     2581 aattgatttc atcatctgtt aaactaaact catcattgac agatcgtgag atataactgt
     2641 ttttaacttt actctttacg ttgactttat tgacagaatt aacattcaca tatcttgatt
     2701 ttaatgtcca tgatgttgtt tgcgtgagaa cattctcagc attaaggaat ttttttaacg
     2761 gaatccttgg tttctttttc gagtccgaat ttacaatatc atgcatatag accatttcat
     2821 gaatctgcga aaattcaatg tccattgtat acctcacatt tttaccgtga ctcgatgtta
     2881 ctgttcaata atcaccttcc atcaatacta aaattaatac ccctaatgtg ccgataacaa
     2941 atatagtcat tctacgtaac gtctccataa ggtgatattt gacattatca gaagttgcga
     3001 attcggattt tgctctaatc aaatgagaga aatgtaaaat gaagccacga aatattaata
     3061 atagcctacc actgcaacca ttagttcctg atcaggagaa caaaaataag aaaaatgaag
     3121 agaaatccgt taatccagtt aaaatcacaa tggggtctgg tttaaattat attgaacaag
     3181 aatctcttgg aggaaaatat ctaacacatg atttgtcaat aaagatagcg gatatttctg
     3241 aagagataat tcagcaagca atattatctg ctatgagcat atataaattt tcgataacag
     3301 acgatttaat gagtatggct gtaaatgaac tcataaaact gaccaaaata gagaataatg
     3361 tagacctgaa taaattcact actatatgca cagacgttct atccccccgc gtcaccagac
     3421 ataataaaga aaagaaacaa acgacattct acccttctca aaaatcccct ttttaatttt
     3481 cattgaaaaa atggcaattg atactatgaa t
//
LOCUS       DAFNBJ010000279         3468 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.280, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000279 DAFNBJ010000000
VERSION     DAFNBJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.280"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            127..1407
                     /locus_tag="L2X49_004823"
     CDS             127..1407
                     /locus_tag="L2X49_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain protein"
                     /protein_id="HBN4799770.1"
                     /translation="MANTTVLPGDVFEIDSTVADVHLISSLNRRKVIGRPTIYTVVDR
                     ATRMIVGLHVSLYHASWRAARQALANCFMPKKEYCRLFGISITDDDWPCSHIPLTLMC
                     DNGEMIGLKPQEEMTPLTKLEFAPVGRGDRKSIVERCFGILNDEVIHRLIGTTRRGKI
                     VKGEPTPQSRACLTIQEVTSLLIREILAHNQRTYEELAYINPLLIENDLVISPKNSWM
                     ISLKHGRFSARAVGADEVIARLLIPVNANITAGGIQYNNLFYECDPEIASGVRVFGRT
                     TCEARIDDNCVDYIYVRFDKNSIFKKHYLLKKRDVFKGKAHLDTDVMADWVDTQKEIN
                     LFTLDSLSNINNKDEFNRKGNERLNEIYESRRVHGKDIKKNRKNELDSLGRTHVANGR
                     SEPLLPSSSNVLLLPGREEKQRWLKGKKKTEDAE"
     gene            1407..2855
                     /locus_tag="L2X49_004824"
     CDS             1407..2855
                     /locus_tag="L2X49_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN4799771.1"
                     /translation="MNLIRRVSAIYKEQELPEYRGNPLIEALPEALTEDEVLLEMSYF
                     PEIDEKIRWTAPANVREQYVERIKKFRCPQTNLIQAYKMILRALRESYAARNPLKSGT
                     IQYLHYYGNERPDIEPESGYFKSQAETITIVGMSGSGKTTMIEQVMDHFPQIIEHSSY
                     KGVFPGFSKQIVWVKINCPYNSSVRDLCEEILQKLDDAIGIERTTPEIRNGALARQIA
                     QRIKSSFLGILVIDEMQRLKFSRTGGESKLIDFLHEIVDSMGVSMVFCGNHPFDETLT
                     KKMRIARRAESGGYMKIKNVRYDSQDWQSFIHYLWPLQWTNVETPLNDELNEKLFILS
                     KGNIGLAQMIYRGAQLKVIGSGNEIITGAVLSASVPVLVSHTEEYKDTPLPGAATEDE
                     EAKWLSASNEGDASAKIMAEKSLKSLIPGDIDRPQHKEFVGKIDEVLRNFDEKILSLD
                     HDLVINRTAEKKNTYDALQRCGLINIDPLNKL"
     gene            2896..>3468
                     /locus_tag="L2X49_004825"
     CDS             2896..>3468
                     /locus_tag="L2X49_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HBN4799772.1"
                     /translation="MRMLPVLPDESLFSRFCRTTTVYGMSPSSLLTIIFNKPDMNVHP
                     ILNSGLKAISLHTSESADQLWHEQTLLPLFAWALPISRNEIMDFNTTPARLNRLCRLS
                     NFSLGQRTLLKFCPVCAREDTFHYGVTYWHLAHQLHGVTTCHRHPVALESIHVPSSPH
                     IRIGLMPPVSYTEQLSNEIDFDFAKFCYESL"
BASE COUNT         1085 a          635 c          764 g          984 t
ORIGIN      
        1 ccatatgttc cttctttaaa acaattttca tactggaata aaaaactctt cactaaagat
       61 ttctcgataa ataagaagaa cacgaaaaaa gaaatagacc tcaaaatgcg ggcgctttta
      121 ggtagtgtgg ctaatacaac tgttttgccg ggtgatgttt tcgaaataga ctcaactgtc
      181 gctgatgtac atcttatttc gagtttaaac agacgaaaag tcattgggcg tcctactatt
      241 tatacagtgg tggatcgtgc aacaagaatg attgttggcc ttcatgtttc tttataccat
      301 gcttcatggc gagccgcccg acaagcgtta gcaaattgct ttatgccaaa aaaagaatat
      361 tgtagattat ttggaatctc tattactgat gatgattggc cttgttctca cattccatta
      421 acattgatgt gtgacaacgg ggagatgatt ggtcttaaac ctcaggaaga gatgaccccc
      481 ctgacaaaac ttgagtttgc gccagtcggt agaggcgaca gaaaaagcat tgttgaacgc
      541 tgtttcggca ttctgaatga tgaggttatt cataggctta ttggaacaac acgaagggga
      601 aagattgtta aaggagagcc aacaccgcaa tccagggctt gtttaacgat tcaggaggtc
      661 acgtctttac tgattcggga gatacttgca cataatcaga gaacgtatga ggaactggct
      721 tatatcaatc ccttactgat tgaaaatgat ctcgtaatat caccaaaaaa tagttggatg
      781 ataagcttaa agcacgggag attcagcgca agagccgttg gggccgacga agtgatcgca
      841 cgattgttaa tccctgtgaa cgctaacatt accgccggtg ggattcagta caataatctt
      901 ttttatgaat gtgatcctga aattgcatca ggtgtcagag tatttggaag aaccacttgt
      961 gaagcaagaa tagatgacaa ttgcgtagat tatatatatg taagatttga caaaaatagc
     1021 attttcaaaa aacattacct acttaagaaa agagatgtat ttaaagggaa agctcatctt
     1081 gatacagatg taatggctga ttgggtagat actcaaaaag aaattaattt atttactctg
     1141 gattcgctca gcaatatcaa taataaagat gaatttaaca ggaaaggtaa cgaaagatta
     1201 aatgaaatat acgaatctcg ccgggtacat ggtaaagata ttaaaaagaa caggaaaaat
     1261 gaacttgatt cattaggacg aactcatgtt gctaatggaa gatctgaacc cctacttcct
     1321 tcatccagta atgttctgct tttgcctggc cgggaggaga aacaaagatg gttaaaaggt
     1381 aagaagaaga ctgaggacgc agaataatga atcttatacg gcgcgtttca gcgatatata
     1441 aagaacagga gttacctgag tatcgtggca atcccttaat tgaagcactt cctgaagcgc
     1501 ttacggaaga tgaagtactt ctggaaatga gttatttccc cgaaattgac gaaaaaatcc
     1561 gctggactgc cccggcgaat gttagagagc agtatgtcga acgtataaag aaattccgtt
     1621 gccctcaaac caatcttatc caggcttata agatgatctt acgggcactt agggaaagct
     1681 atgcagctcg taatccttta aaaagcggaa caattcaata tcttcattac tatggaaatg
     1741 agcgtcctga tattgagcca gaaagtggat attttaaatc ccaggctgaa actattacga
     1801 tagttggaat gagtggttct ggaaaaacta ccatgattga gcaagtcatg gatcatttcc
     1861 ctcagattat agaacacagc agctataaag gagtttttcc cggcttcagt aagcaaattg
     1921 tatgggtaaa gattaattgt ccatacaatt caagtgtgag agacctttgt gaagagattt
     1981 tacaaaaatt agatgatgca attggtattg aacgaactac acctgagatt agaaatggtg
     2041 ctctggctcg ccagattgca caaagaatta aatcatcatt tttgggtatc cttgttattg
     2101 atgaaatgca gaggcttaaa ttttcaagaa ccgggggtga gagtaagttg attgattttt
     2161 tacatgaaat tgtagattcc atgggggtat ctatggtttt ttgtggaaat catccttttg
     2221 acgagacgct gacaaagaaa atgagaattg ccaggcgggc agagtctggt ggttacatga
     2281 aaattaagaa tgttagatac gattcacaag actggcaatc gtttattcat tatttatggc
     2341 cattacaatg gacaaatgtt gaaactccat taaatgatga gttaaacgaa aaattattca
     2401 ttttatctaa aggcaatatt ggattggctc agatgattta tcggggggca caattaaagg
     2461 ttatcggtag cggcaatgaa atcatcactg gcgcagtctt gtcggcctct gtaccggtac
     2521 tcgtgagtca tacggaggaa tataaggata ctccactccc gggggccgcg actgaagatg
     2581 aggaagctaa atggctctct gcatctaatg agggtgatgc ttcggctaaa atcatggcag
     2641 agaagtccct aaagtctttg atccctgggg atatagacag gccacagcac aaggaattcg
     2701 taggaaaaat agacgaggtt ttgcgcaatt tcgacgagaa aatactgtct cttgaccacg
     2761 atcttgttat caacagaaca gctgaaaaga aaaataccta cgatgctctt cagcgttgcg
     2821 ggcttataaa tatagatcca cttaataaat tgtaatgagg taacagttcg taacggtcaa
     2881 agagaggtgt gggaaatgag aatgttgcct gttttgcctg atgaatcctt gttcagccgg
     2941 ttttgtcgga caactaccgt gtacggtatg tccccatctt ctctgttaac gatcattttc
     3001 aacaaacctg atatgaacgt ccatccaatt ctcaattcag gattaaaggc tatttctctt
     3061 catacatccg aaagtgcaga tcagctctgg catgaacaga ctttactccc tctttttgcc
     3121 tgggcactac caatcagtcg taatgaaatt atggatttca acacgacccc tgccaggctt
     3181 aatcgattgt gccgccttag taatttttca ctagggcagc gcacactatt gaagttttgc
     3241 ccggtttgcg ctcgtgaaga tacttttcat tatggtgtta cttattggca tctggcgcat
     3301 cagcttcatg gggttacaac ctgccatcgg catccggtag cgcttgaaag catccatgtc
     3361 ccttcttcac cgcacatacg tattggactg atgcctcctg tttcgtatac agaacaactt
     3421 agcaatgaga tagacttcga ttttgctaag ttttgttatg agtcccta
//
LOCUS       DAFNBJ010000280         3416 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.281, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000280 DAFNBJ010000000
VERSION     DAFNBJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.281"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..104)
                     /locus_tag="L2X49_004826"
     CDS             complement(<1..104)
                     /locus_tag="L2X49_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBN4799773.1"
                     /translation="MASISIRCPSCSATDGVVRNGKSTTGHQRYLCSH"
     gene            172..1109
                     /locus_tag="L2X49_004827"
                     /pseudo
     CDS             172..1109
                     /locus_tag="L2X49_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491235.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1269..2073
                     /locus_tag="L2X49_004828"
     CDS             join(1269..1619,1621..2073)
                     /locus_tag="L2X49_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611700.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HBN4799774.1"
                     /translation="MAGNKWQISDELWEKMAPLLPEHKTHHPLGTHRRRVNNRAAMNA
                     ILFVLRTGCQWNALNATGICSSSSAHRRFQEWRDAGVFERFWQNGLLACEQLDAIDWS
                     WLSMDGCMTKSPLAGQKTGRNPTERGKQGVKRSLMTDANGLPLSLVVAGANTHDIKLV
                     ADTLDALQTGRPGRRLHLCMDKGYEAEWLEACLKNRRYEPHIQSRKEESEAIKSTDFK
                     AHRWVVERTHSWMNRFRRVLTLWEKKVENYVAMLYLTCGVIVWNKILLG"
     gene            2302..>3416
                     /locus_tag="L2X49_004829"
     CDS             2302..>3416
                     /locus_tag="L2X49_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBN4799775.1"
                     /translation="MRIVYFVNAAWYFELHWLDRSEAAISKGYEVHLVSNFADDAIKN
                     NLEKKGIKCWDIELNRFSKNVFKNISIFTAFRKICKQIKPDLIHLITIKPILFGGLYA
                     RVAGIPFVVSFVGLGRLFGNKRGWLNKFIFNSVLSIYSLLLSAKSPAQVIFEHNADFA
                     ELSKYIKFDENNIHIIEGAGVDRFRFSYQPEPTQNNFSVLFASRLLWSKGLGEVVEAI
                     GKVKQNENNTTLYVAGILDDKDPDRIELDQILQWEKEGKIIWLGRRDDIQNLIRNSHV
                     VILPTKYSEGVPRIIIEACAMGRSCIVGNVPGCKAIIKDNFNGCVLKTHSANEIAEKI
                     EYLRDNMEIRKKFGLRSAEIVKERFSKEIVIDKTLCV"
BASE COUNT          984 a          721 c          879 g          832 t
ORIGIN      
        1 cagtgagagc agagatagcg ctgatgtccg gtggtgcttt tgccgttacg caccaccccg
       61 tcagtagctg aacaggaagg acatctgatg gaaatggaag ccacgcaagc accttaaaat
      121 caccatcata cactaaatca gtaagttggc agcattaccc atcgtgtcac catggcaaag
      181 caaaagttta aaatcaccaa ctggtcagct tacaacaagg cactcagaca gcgtggttcg
      241 ctgacgatct ggcttgatga atctgctatt gctgcatgga ctgactgcgc aaagcctgaa
      301 ggtcgtggtc ggcctctcca ctacaccgat atggcgatca ccacggttct gatgatgaag
      361 cgcgtgttta atctgccact ccgggcatta cagggcttcg ttgactcgat ttttacgctg
      421 atggctctgc cgctgcgttg cccggactac tctctggtca gcaaacatgc aaagagcgtt
      481 aacatcagca taaaaacgcc aacccggggt gaaatctcgc atctggttat cgatgctact
      541 ggcctgaagg tcttcgggga aggcgaatgg aaagtcaggc aacatggggc tgacaggcgc
      601 agagtatggc gcaaacttca tctggctgca gacggtgtga cgcatgagat aatctgtgcc
      661 gatttatcgc tcagcggtac gacagatgcg caggcactgc cggggctgat taaccagacc
      721 caccgaaaaa taagagaagc ttcagcagac ggcgcttatg acacgcgcaa ctgtcatgat
      781 gcgttgctga ggaaaaaaat caggccactt atccctccac gaaacggggc gcaatactgg
      841 ccgggcagat accatgaacg taaccatgcg gtggctaatc agcatctgag cggtggcaat
      901 gatgtatgaa aaaagaaagt aggctaccac cgacgttccg tggcagaaac ggcgatgttc
      961 cgtatcaaaa ccttgctggg cggacacttg agcctgcgag actacgatgc gcaggtgggg
     1021 acgcgcaggt gggcgaagcg atggcgatgg ttaaagcgct aaaccggatg acgttgttgg
     1081 gtatgccgca tagtgtccgg atcgcataac gggtgtactg ctggccccct cttcggctga
     1141 ctctgattta ttcaacaaag cctgtcagga gctgactgcc atgatgcttg tttttttatt
     1201 catcttatta agaacctatc ccaaaaggaa ttgtcagtta ataagtaatg acaatttctg
     1261 agggaagtat ggcaggtaac aagtggcaga tcagcgatga actctgggaa aaaatggctc
     1321 cgttgctccc ggaacacaaa actcaccatc cgctgggtac gcatcgcagg cgggttaata
     1381 atcgtgccgc aatgaacgcc attctctttg tgctcagaac gggttgccag tggaatgccc
     1441 tgaatgccac cgggatatgc tcgtcaagct ctgctcaccg ccgcttccag gagtggcggg
     1501 atgcaggagt ttttgaacgg ttctggcaaa acgggttgct ggcctgtgag cagttggatg
     1561 ccatcgactg gtcatggctg tcgatggatg gctgtatgac gaagtcaccg ctggctggct
     1621 caaaaaacag gacggaaccc cacagaacga gggaaacagg gcgtaaaacg tagcctgatg
     1681 accgatgcga acgggcttcc cctctcattg gttgtcgcag gtgcaaatac ccacgacata
     1741 aagctggttg ctgatactct cgatgccctt cagacgggca ggcctggcag aaggctccac
     1801 ctctgtatgg acaaaggcta tgaagccgaa tggctggagg cctgtctgaa aaaccgccgt
     1861 tacgaacctc atatccagtc aaggaaagag gagtcggaag ccatcaaaag cacggatttt
     1921 aaagctcacc gctgggtcgt ggagagaacg cacagttgga tgaatcgctt ccgtcgggtg
     1981 ttgactctct gggagaagaa agtcgaaaat tatgtggcaa tgctgtatct tacctgcggc
     2041 gtcattgtct ggaataaaat cctattggga taggctctaa gattattaca ctcaaaatac
     2101 aacccgttgt cttgagtaat actacgctgc aatgggagtg ttaggcttag ggtagttgca
     2161 gcggtcagta gcctggccgt caatactcct gacaaaaaac aagagtttcg ccctttggca
     2221 gccagagcca agggcggtag cgtgcctaca ttacatgtag taagccgtga ttgaccatct
     2281 cgcgagttgt tgaggaatgg tatgaggata gtgtattttg tcaatgcagc gtggtacttc
     2341 gaactgcatt ggttggaccg gtcagaagcg gcaattagca aagggtatga agttcatctt
     2401 gtaagtaatt ttgctgatga cgccatcaaa aataacctgg aaaaaaaagg tataaaatgt
     2461 tgggatattg agcttaacag attttctaaa aatgtgttta aaaacatttc aatattcact
     2521 gcatttagaa agatctgtaa gcaaattaaa cccgatctta ttcatctaat tactataaaa
     2581 ccaatccttt ttggtggatt gtatgcccgg gttgcaggca tcccttttgt cgtgagcttt
     2641 gtgggattag ggagattgtt tggcaataaa cgaggttggt tgaataaatt cattttcaac
     2701 tcagtactgt caatttatag tcttttgcta tcagcaaaaa gcccagcgca agtaatattt
     2761 gagcataatg ctgactttgc tgagctgagt aaatatatta aatttgatga aaataacatc
     2821 cacatcattg aaggagccgg ggtagacagg tttcgcttta gttatcagcc agaaccaact
     2881 cagaataatt tctcggtatt atttgccagc cgtttgctat ggtcaaaggg gctcggtgaa
     2941 gtagtcgagg ccatcggtaa ggttaaacaa aatgaaaata acacaacctt atacgttgcg
     3001 ggaattctcg atgacaaaga tccggataga attgaattag atcaaatttt gcaatgggaa
     3061 aaagaaggaa aaatcatttg gttaggtcga agagatgaca tccaaaattt aatcagaaat
     3121 agccatgtcg tgattttgcc aactaaatat tcagaaggcg tgccaagaat aatcattgaa
     3181 gcgtgtgcta tgggccgaag ttgcattgtc ggaaatgtac ccggctgtaa agccataatt
     3241 aaagataatt tcaatggatg tgtactaaaa acacatagcg ctaatgagat tgcggagaaa
     3301 attgaatatc ttagagataa catggaaata agaaaaaaat tcggtctacg ctcagccgaa
     3361 attgtaaaag aaagattcag caaagaaatt gttatagata aaacgttatg tgttta
//
LOCUS       DAFNBJ010000281         3395 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.282, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000281 DAFNBJ010000000
VERSION     DAFNBJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.282"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            88..1239
                     /gene="yiaY"
                     /locus_tag="L2X49_004830"
     CDS             88..1239
                     /gene="yiaY"
                     /locus_tag="L2X49_004830"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBN4799776.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1404..2942
                     /gene="aldB"
                     /locus_tag="L2X49_004831"
     CDS             1404..2942
                     /gene="aldB"
                     /locus_tag="L2X49_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBN4799777.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3161..3364)
                     /locus_tag="L2X49_004832"
     CDS             complement(3161..3364)
                     /locus_tag="L2X49_004832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799778.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
BASE COUNT          839 a          831 c          904 g          821 t
ORIGIN      
        1 ttttgataat caataataat gatcatgttt tcgaatgaaa atcctcagta agctgcccac
       61 ccttttttac actttcagga gtgtgttatg gcatcttcaa ctttctttat tccttctgtg
      121 aatgtcatcg gcgctgattc attgactgat gcaatgaata tgatggcaga ttatggattt
      181 acccgtacct taattgtcac tgacagtatg ttaacgaaat taggtatggc gggtgatgtg
      241 caaaaagcac tggaagaacg caatattttt agcgttattt atgatggcac ccaacctaac
      301 cccaccacgg aaaacgtcgc cgcaggtttg aaattactta aagaaaataa ttgcgatagc
      361 gtgatctcct taggcggtgg ttctccacac gactgcgcaa aaggtattgc gctggtggca
      421 gccaatggcg gcgatattcg cgattacgaa ggcgttgacc gctctgcaaa accgcagctg
      481 ccgatgatcg ccatcaacac cactgcgggt acggcctctg aaatgacccg tttctgcatc
      541 atcactgacg aagcgcgtca tatcaaaatg gcgattgttg ataaacatgt cactccgctg
      601 ctttctgtca atgactcctc tctgatgatt ggtatgccga agtcactgac cgccgcaacg
      661 ggtatggatg ccttaacgca cgctatcgaa gcatatgttt ctattgccgc cacgccgatc
      721 actgacgctt gtgcactgaa agccgtgacc atgattgccg aaaacctgcc gttagccgtt
      781 gaagatggca gtaatgcgaa agcgcgtgaa gcaatggctt atgcccagtt ccttgctggt
      841 atggcgttca ataatgcttc tctgggttat gttcatgcga tggcgcacca gctgggcggt
      901 ttctacaacc tgccacacgg tgtatgtaac gccgttttgc tgccgcatgt tcaggtattc
      961 aacagcaaag tcgccgccgc acgtctgcgt gactgtgccg ctgcaatggg cgtgaacgtg
     1021 acaggtaaaa acgatgcgga aggtgctgaa gcctgcatta acgccatccg tgaactggcg
     1081 aagaaagtgg atatcccggc aggcctacgc gacctgaacg tgaaagaaga agatttcgcg
     1141 gttctggcga ctaatgccct gaaagatgcc tgtggtttta ctaacccgat ccaggcaact
     1201 cacgaagaaa ttgtggcgat ttatcgcgca gcgatgtaat catcatttcc acaacggctg
     1261 gcaaattgtc agccgctttt tcaactatct ctgtaaccct tgcccgtaaa ttcgtgatag
     1321 ctgtcgtaaa gctgttaccg actggcgaag atttcgccag tcacgtctac ccttgttata
     1381 cctcacaccg caaggagacg atcatgacca ataatccccc ttcagcacag attaagcccg
     1441 gcgagtatgg tttccccctc aagttaaaag cccgctatga caactttatt ggcggcgaat
     1501 gggtagcccc tgccgacggc gagtattacc agaatctgac gccggtgacc gggcagctgc
     1561 tgtgcgaagt ggcgtcttcg ggcaaacgag acatcgatct ggcgctggat gctgcgcaca
     1621 aagtgaaaga taaatgggcg cacacctcgg tgcaggatcg cgcggcgatt ctgtttaaga
     1681 ttgccgatcg aatggaacaa aaccttgagc tgttagcgac ggctgaaacc tgggataacg
     1741 gcaaacccat tcgcgaaacc agtgctgccg acgtgccgct ggcgattgac cattttcgct
     1801 atttcgcctc gtgtattcgg gcgcaggaag gtgggatcag tgaagttgat agcgaaaccg
     1861 tggcctatca tttccatgaa ccgttaggcg tggtggggca gattatcccg tggaacttcc
     1921 cgctgctgat ggcgagctgg aaaatggctc ccgcgctggc ggcgggcaac tgtgtggtgc
     1981 tgaaacccgc acgtcttacc ccgctttctg tactgctgct aatggaaatt gtcggtgatt
     2041 tactgccgcc gggcgtggtg aacgtggtca atggcgcagg tggggaaatt ggcgaatatc
     2101 tggcgacctc gaaacgcatc gccaaagtgg cgtttaccgg ctcaacggaa gtgggccaac
     2161 aaattatgca atacgcaacg caaaacatta ttccggtgac gctggagttg ggcggtaagt
     2221 cgccaaatat cttctttgct gatgtgatgg atgaagaaga tgcctttttc gataaagcgc
     2281 tggaaggctt tgcactgttt gcctttaacc agggcgaagt ttgcacctgt ccgagtcgtg
     2341 ctttagtgca ggaatctatc tacgaacgct ttatggaacg cgccatccgc cgtgtcgaaa
     2401 gcattcgcag cggtaacccg ctcgacagcg tgacgcaaat gggcgcgcag gtttctcacg
     2461 ggcaactgga aaccatcctc aactacattg atatcggtaa aaaagagggc gctgacgtgc
     2521 tcacaggcgg gcggcgcaag ctgctggaag gtgaactgaa agacggctac tacctcgaac
     2581 cgacgattct gtttggtcag aacaatatgc gggtgttcca ggaggagatt tttggcccgg
     2641 tgctggcggt gaccaccttc aaaacgatgg aagaagcgct ggagctggcg aacgatacgc
     2701 aatatggcct gggcgcgggc gtctggagcc gcaacggtaa tctggcctat aagatggggc
     2761 gcggcataca ggctgggcgc gtgtggacca actgctatca cgcttacccg gcacatgctg
     2821 cgtttggtgg ctacaaacaa tcaggtatcg gtcgcgaaac ccacaagatg atgctggagc
     2881 attaccagca aaccaagtgc ctgctggtga gctactcgga taaaccgttg gggctgttct
     2941 gatataagaa gctggtcgca ttgggtattc attgcctgat gcgacgctta cgcgtcttat
     3001 catgcctacg ggaacctgac cgtaggccgg ataaggcgta cacgccgcat ccggcataaa
     3061 caaagcgcac gttgttacaa tttacgtttt aaacctacgt gcgcaataac ccagactacg
     3121 ccaacagaag tgcgtttctg gggcgtgttt tgccaggagt ttactctcgc gtttcgatgg
     3181 tgagcgggca aactaggccg taaatcacca ttggcgttcc gaggccaatc agcaataaac
     3241 ggtagataat tgaaggttta atacgcttta tctgtactgt ggatgtttta ctggagttat
     3301 tcattagctt ggtcaatagt tccattattt caatatgata aacctcgacc tgttgaaaag
     3361 ccacgcagca cacttcagaa agtatataaa aaaca
//
LOCUS       DAFNBJ010000282         3303 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.283, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000282 DAFNBJ010000000
VERSION     DAFNBJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.283"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..752
                     /locus_tag="L2X49_004833"
     CDS             <1..752
                     /locus_tag="L2X49_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HBN4799779.1"
                     /translation="VLEKLEMKWSPEQISGWLRRTKPRQKTLRISPETIYKTLYFRSR
                     EALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEG
                     DLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTWDR
                     GMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHELD
                     LVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(759..968)
                     /locus_tag="L2X49_004834"
                     /pseudo
     CDS             complement(759..968)
                     /locus_tag="L2X49_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1032..1694)
                     /locus_tag="L2X49_004835"
                     /pseudo
     CDS             complement(1032..1694)
                     /locus_tag="L2X49_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1985..2389)
                     /locus_tag="L2X49_004836"
     CDS             complement(1985..2389)
                     /locus_tag="L2X49_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBN4799780.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKNNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRDRGAAGGQLEG"
     gene            2575..2793
                     /locus_tag="L2X49_004837"
                     /pseudo
     CDS             2575..2793
                     /locus_tag="L2X49_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2859..>3303)
                     /gene="istB"
                     /locus_tag="L2X49_004838"
     CDS             complement(2859..>3303)
                     /gene="istB"
                     /locus_tag="L2X49_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HBN4799781.1"
                     /translation="VGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKT
                     TLQRGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTF
                     AGDAALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          867 a          838 c          772 g          826 t
ORIGIN      
        1 ttgttctgga aaagctggag atgaaatggt ctccagagca aatatcagga tggttaaggc
       61 gaacaaaacc acgtcaaaaa acgctgcgaa tatcacctga gacaatttat aaaacgctgt
      121 actttcgtag ccgtgaagcg ctacaccacc tgaatataca gcatctgcga cggtcgcata
      181 gccttcgcca tggcaggcgt catacccgca aaggcgaaag aggtacgatt aacatagtga
      241 acggaacacc aattcacgaa cgttcccgaa atatcgataa cagacgctct ctggggcatt
      301 gggagggcga tttagtctca ggtacaaaaa actctcatat agccacactt gtagaccgaa
      361 aatcacgtta tacgatcatc ctcagactca ggggcaaaga ttctgtctca gtaaatcagg
      421 ctcttaccga caaattcctg agtttaccgt cagaactcag aaaatcactg acatgggaca
      481 gaggaatgga actggccaga catctagaat ttactgtcag taccggcgtt aaagtttact
      541 tctgcgatcc tcagagtcct tggcagcggg gaacaaatga gaatacaaat gggctaattc
      601 ggcagtactt tcctaaaaag acatgtcttg cccaatatac tcaacatgaa ctagatctgg
      661 ttgctgctca gctaaacaac agaccgagaa agacactgaa gttcaaaaca ccgaaagaga
      721 taattgaaag gggtgttgca ttgacagatt gaatctacaa gttgcagaat ttcagattcc
      781 agttcagcca ccgtgcggga accaggagta ccgagccctt ttctggcggc ggtaacccat
      841 tgtcctaaag tgccttcagg aagagataat cgggaagcgc cttcactgat cgaaagttga
      901 ttttcaagaa ccgttctgac agcttcggct ttgaactctt tagagtaacg ttgggttttt
      961 ctgctcatta ttagctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
     1021 caggctacct cagctgtcgc atagactcca ctgcgatcac gcgatacaac ctgtatttcc
     1081 ggaccttttc tgaaccaggt cgccagcgta cgctgatcac ggccgggaag caggaccagg
     1141 gggcggtgag tatcaagatt gaatattaac gtaccgtaac ggtggcccca atgccacgcc
     1201 cactcatcaa taccgacatg aggcgcgcct gactgtttcg tctccggggt attgataagc
     1261 ctgcgaagaa gagtatcagc actgcaacgg agacccgctg ccgttgcagc ccgttttcca
     1321 gcctcacctc cagctattaa tcccagttga cgttgttaat tttgtaacgc ctgtgaagac
     1381 tgctggtgtg aactggcgaa gggagcaagc gactcggcaa aaattttacg tgaacaagcc
     1441 gggttacggc agtaccagtg gcggacggaa aatacaagcc atagcgtctg ccctgagcag
     1501 ggtagatgct gtatccggcg ccgacgacaa ctatgaacag agtcgctacg cttaaagcat
     1561 tcaggacatt gtgctgtttt gcgttttccg tggagatgga gggttatacc gtcagagcta
     1621 ataatttgtc gactgcatcg ccatccgcaa ggtaacttta gaagtgtctt aagagagtgc
     1681 attgcgttgc ccataactta aatcagctta gatctttgtt caccagaaag tttcatccat
     1741 tttcttccac ccaagttgcg gaagaaccac ttttcatccg tcgttgacag ctgtgaagta
     1801 tgcgggagaa aaagcgaata tctaactgta ataactcagg ccggtacgag tactcgggac
     1861 gtctacaatc attcagggca aagtgctaaa atttctctat cccgggatgc ccctaccaac
     1921 gcagcactcc cggagatatc attacagctc tgcaactgat ttccgcgact gaaccgctca
     1981 gttcctatcc ttccagttgc cctcctgcag cccccctatc cctgctgtat accccggtac
     2041 cacaacgcgg gcagcacttt tccccctcat aatcttcgtc acacatcaca caaaaccact
     2101 ttctcacctt caccgtataa tccggaaccg tcatgccctg cagatgcaaa cgacgggcct
     2161 ctttctctgc cgggtcaagc caggtgcatc gcagcaggat tttcgcagca acagtcatct
     2221 ggtcatgttc aaaccacggc ggttgtccgt cattgccccg cttaagtgtt aacggacaac
     2281 cacaggacac acagcacaaa acgtcctgtc ggttgttctt cgcggcttct ttcgccgtaa
     2341 caaaatggcc tttgctgtca ttcgccatat agcagttcag aattcgcata tctcctcctt
     2401 gtatcttgac aattggttaa aataactgtg attagatttg gcaaggtgta tgcagcccga
     2461 tccctcaagg ggttcatagc ccgtagctca gcgaatggcg catacactga gccactcatc
     2521 tgaaacccga acagttgtca gtacgccata acgcatttgc aaggatgggt aatcatgagt
     2581 cagccaggtt cactttgtgt gggtatcgat gtttcgaaag cgatactgga tattgccgcc
     2641 agcagcgcta ttgaacaatt ttctgtcggc aatgacagtg atggctttga ggccatcatt
     2701 gctgagttga gaatgtacgc cattgcgctg gttttaatga aagccactgg aggacttgaa
     2761 accgccatag tctgtgcgct tcaggcagaa ggtgtcaacg acggatgaaa agtgatccac
     2821 ttatatctcc accaacggcc caatattgat ccaccgtttt actcaggatt agcttctgct
     2881 ataaccccgg cctttcgttt ctgtctgagt cgatagcttt ctcctttgat ttgaacgaca
     2941 tgtgagtggt gtaagatacg gtccagcatc gctgaggtca gtgctgcatc accggcgaac
     3001 gtttgatccc actgcccgaa cggcagattg gatgtcagga tcattgcgct cttttcgtaa
     3061 cgtttagcga tgacctggaa gaacagcttt gcttcttcct gactgaacgg cagatagcct
     3121 atttcatcaa tgatgagcag gcggggggcc attactccac gctgaagcgt cgttttataa
     3181 cggccctgac gttgtgccgt agataactga agtaacagat ctgctgctgt tgtgaagcga
     3241 actttgatac ctgcacggac tgcttcatag cccatcgcta ttgccagatg ggttttcccc
     3301 aca
//
LOCUS       DAFNBJ010000283         3249 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.284, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000283 DAFNBJ010000000
VERSION     DAFNBJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.284"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            236..1114
                     /locus_tag="L2X49_004839"
     CDS             236..1114
                     /locus_tag="L2X49_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799782.1"
                     /translation="MKLATDLVNAVLIKNGIVPTSINELPVTSLVKPLTQVHLSLYLK
                     TTHLSQFVPDYVESLKDCPVSYDIGLELQQCINSAELLCEDMESLEFISVWIELYTCN
                     SKQQQFEKSNFSFRTEDIELAKMLSCSHEILISQAACFWLLYFEDFMKYVRNRLCALL
                     RQVLILIAGLRNVYLQRNEDQLIKRVKHGNEMLEVTLKAVALCSIERYLKSYASLRTE
                     AAASGFIKCIQSYISTGWRHGYIVCQYSKNGRSLGKSLPLFIKETSEHNIQKRVNRLI
                     SSTISGRNKDLGLYDF"
     gene            1185..2119
                     /locus_tag="L2X49_004840"
                     /pseudo
     CDS             1185..2119
                     /locus_tag="L2X49_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2197..3117
                     /locus_tag="L2X49_004841"
     CDS             2197..3117
                     /locus_tag="L2X49_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799783.1"
                     /translation="MKAIYKIIEAMLVKMGFEGAVIQSVNLETGVIQSRVVKADIPVR
                     TLMPHLRRYLRDCEPLWGHDVDYHPIAQYCSGAALLKAGPVTLETFRFEVSVSTAHLR
                     LVDIEPDCLNEGVNREKLEYVYYRSSNADKDLILGMVEKGHNFINHLHELLRSRLSTA
                     LPLIFAVTEKIAESSVPQVVTSFFSVDHQINAQIVVEPVPVSVADQIISADWNVCSVS
                     GADKFLNHVNRYLSGRVMKRRIYAVIEACDGGSLSLNANLGCFTSPLLVTEYKMVQSH
                     SSSLYRKAINNSLKQVNSYAPEHSDDLLSM"
BASE COUNT          905 a          601 c          721 g         1022 t
ORIGIN      
        1 ttgattattt aaaacttgtt attgatgatc ttcgtgcgcg agggtatgcc gatttccttc
       61 cagcatacaa ccttgatggc cttcgtcact ttgcagtaaa ggatgagcga ccatcacttg
      121 tcagttatat cgacaatgtc atggcacttt gcgcataaag ataacacata tttttactac
      181 atccctgatg gcacctccca tcggggtggc cctcagagtt gaatctggag gtcttatgaa
      241 attagctaca gatctggtca atgcggttct gataaagaat ggcattgtac caacttccat
      301 taatgagttg cctgtaacgt cgctggtaaa gccattgaca caagtgcatc tcagcctgta
      361 tctgaaaaca acgcatttat ctcagttcgt ccctgactac gttgagtctc tgaaagattg
      421 tccggtttcg tatgatatcg gccttgaact tcagcagtgt atcaacagtg ctgaattgct
      481 ctgtgaagac atggagtcac ttgagtttat ctctgtatgg atagagctct atacgtgtaa
      541 ttcaaagcaa cagcagtttg agaaaagtaa cttttctttt cgaactgaag atatcgagct
      601 ggctaagatg ctgtcttgct cgcatgaaat cttaataagt caggcggctt gtttctggct
      661 tttgtatttt gaagatttca tgaaatatgt tcgtaatagg ctctgtgcat tactcagaca
      721 ggttttaatt ttgattgcag gactgcgaaa tgtatacttg caacggaatg aagatcagtt
      781 gattaagaga gttaagcacg ggaatgaaat gctggaggtg actcttaaag ctgttgcact
      841 atgttcaata gaaaggtatc tgaaatcata tgcctcttta cgaacagaag cggcggcttc
      901 tgggtttatc aaatgcattc agtcttatat cagtactggt tggagacatg gctatatagt
      961 ctgtcaatat tcaaaaaatg gacggtctct tggtaagtca ttaccacttt ttataaaaga
     1021 gacatctgag cataatatac agaaacgtgt caatcgcctt atatcttcca cgatttctgg
     1081 gcgaaataaa gatttaggtc tttacgattt ctgattaaca ttacccatcc ggggattaca
     1141 tccctgatgg gtgtaatccc attttttatt taagtgagga ttacatgaaa attattgaga
     1201 aaatcattaa tgcatttctg atatcgcgta aacattcagt gaagatcagt aatgttatct
     1261 gcttgagtgg caccgacggt aaatttactg gaatatgctg tgacgccgat gtttcatttg
     1321 attttcttta ttcgtatgcc cccgcataca gttcaacatt tttagatatc ccgtttcctg
     1381 ggtttgaaga ccaggacatc gcggattgtg ttaaatgtca gttagatgta gttaaaaata
     1441 aacggaatcg gtctttttta attgaccata ttagatttcc agtttcctca agggaaggat
     1501 tcactctgac acgcggtgat agttacgacg tgactgaatg tgaatataat aaggaacgat
     1561 tgctacatct gacccgacaa ggacgttttt gtgaagacta tctcacgttc aaggatggcc
     1621 tgagttcctt cttttcattt gtgaatttcg aattacatga aatagtgaag gaaggtatac
     1681 gtttggcact tgatgtttta aacaagatta catctgatca accagaccgt cttataaaag
     1741 actttaagta tcatgattgc ttcggcagtt ataatgtaca aatattttct aagggtctgc
     1801 cagttgatat ccttgaaacg atgattgcac ctgataggtt gttgtctaac ttgtcaggta
     1861 gtcgtcagat tatgaaaaat gcaagtcgct atcttaaagg ctttgccctc agtaatcgtg
     1921 tttacatgaa atacggatgg cgtttgcaga tgttgacaca tctgactatg cgaaagttat
     1981 gtcagataga gaaatgcatg ccagagatga tcttaaggcg ttttgcagtg tcttttttaa
     2041 agagtgtttc gcatccggaa aatatgaata cgaagcatac ctgagtgaaa gacaacacgc
     2101 gattcgtaac gggtattaat acttattatc aacttatcta atccctgagg gaattcttcc
     2161 cttggagagg attccctctt aaccgagggc tacaaaatga aagcgattta taaaataatt
     2221 gaagcgatgc tggtgaaaat gggctttgaa ggagccgtga tccaaagtgt caatctggaa
     2281 acgggtgtga ttcagagtcg agttgttaag gctgatattc ctgtccggac tttaatgccg
     2341 catttaaggc gatatctgcg cgattgtgag cctttatggg gacatgacgt ggattaccac
     2401 cctatagcac aatattgctc aggagctgcg ttgttgaagg ctgggccggt cactcttgaa
     2461 acctttcggt ttgaggtttc agtgtcaact gctcacctgc ggcttgtgga tattgagccg
     2521 gactgtctaa atgagggtgt gaaccgtgaa aaactggagt atgtatatta ccgctccagc
     2581 aatgcagaca aggatctaat acttggtatg gttgaaaaag gacataattt tatcaatcat
     2641 ctgcatgaac ttttacgctc gcgcttatca acggctttgc cgctgatttt cgcagttaca
     2701 gaaaagattg cggagagttc agttccacaa gtagtcacca gctttttcag tgttgaccat
     2761 caaatcaatg cccagatagt agtcgaacct gtacccgttt cagtcgcaga ccagataatt
     2821 tctgctgact ggaatgtgtg tagtgtttca ggagcggata agttcctgaa ccatgttaat
     2881 cgttatctgt cgggtagggt gatgaagcgg cgtatttacg ctgttataga agcttgcgac
     2941 ggtggatctt tatccctcaa tgcgaactta ggttgcttta catcacctct tctcgtaact
     3001 gagtataaaa tggttcagtc acactcttca tcgctttatc gaaaggctat aaataactcg
     3061 cttaagcaag ttaattcgta tgctccagag cattcagatg acctgctttc tatgtaatta
     3121 attttaccca ttggggaagc gtattccccg tgggggttgc ttcctttctt cattaaagag
     3181 gaagtgaata tgtttaatga aagtgatgat aaaaactttg ttagtgcaat gcttaaatgt
     3241 caacttggc
//
LOCUS       DAFNBJ010000284         3174 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.285, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000284 DAFNBJ010000000
VERSION     DAFNBJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.285"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            239..409
                     /locus_tag="L2X49_004842"
     CDS             239..409
                     /locus_tag="L2X49_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799784.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRR
                     AWAAEQARKIHN"
     gene            373..801
                     /locus_tag="L2X49_004843"
     CDS             373..801
                     /locus_tag="L2X49_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799785.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHF
                     EKYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDT
                     FHGKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
     gene            complement(1017..1148)
                     /locus_tag="L2X49_004844"
     CDS             complement(1017..1148)
                     /locus_tag="L2X49_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBN4799786.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLATATGKQDVREM"
     gene            complement(1637..1831)
                     /locus_tag="L2X49_004845"
     CDS             complement(1637..1831)
                     /locus_tag="L2X49_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBN4799787.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEELQNSIQTRFEAENLTGI"
     gene            2115..2438
                     /locus_tag="L2X49_004846"
     CDS             2115..2438
                     /locus_tag="L2X49_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBN4799788.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQVIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            complement(2827..>3174)
                     /locus_tag="L2X49_004847"
     CDS             complement(2827..>3174)
                     /locus_tag="L2X49_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN4799789.1"
                     /translation="IQNEKKLTSVAFNTLLNLIVHWWSLQEWPHPSMESLAIRMGVSV
                     RTVQRAINDLEKANLLDKKPTSKSDRRYGGRNIYDLTKLVDYLDTMGPSVAEQVKKPR
                     HKKPVYTVRKTTA"
BASE COUNT          803 a          724 c          761 g          886 t
ORIGIN      
        1 aaaaaacaat acggtaggca ctgctgtcca gccaagagac aaaccgccag ctttccattt
       61 attcttaacg gagtaagtca ttgattttcc taagccccaa aatatttaaa gtatatatta
      121 tatggtatat tcatatgaat agggtgacac tagcgccatt attgtgcaac caaaaaagac
      181 tactctgaaa acgaggaaaa gattttttcc tgcctgaatt agatacggag ttagcgatat
      241 gaaaaccgaa caacgtcatg atcttgttaa agatattgag gtttttggcg tatccttgtc
      301 tctgttgatt tccagagcga atgagaagtc tgttacaatg ccatctggtc taagtcggga
      361 gcagagaaga gcatgggcag cggagcaggc gcgcaaaatc cacaattgaa tattgtctca
      421 ttctctgaga ccttcaacct ttattacaca tccagatatt ctgcaaaaac actcgataaa
      481 atcgatgatt tcattgagca ttttgaaaaa tacaatctct ttggcgatcc tttaaaagga
      541 tatccagctt ggactggcaa agtatcgcca tcgtggaaag tgcctgatca ttacgaaaac
      601 aaagaagcta ttgagaagta tgctagagct aacaaattat ggcatgctca tttaggcgat
      661 ccggttttta aagatacgtt tcatgggaaa tacaaggttt ctgactgggt tattcatttc
      721 cagcggctga caccgaacca tataaagctt ttagagcttg gataccatga cccaatgaag
      781 ctacctcaaa actttgaatg atcgagcggc aggctaaatg aaatcttgag attcattcag
      841 tctcgtcgta atctcactat tgtaaaaacg aaaaaaccac cctggcaggt ggtttttcga
      901 aggttagtta atcctggcag attctctaac cgtggtaaca gtcttgtgcg agacatgtca
      961 ccaaatactg tcctttcagt gtagcctcag ttaggccgcc acttcaagaa ctctcgttac
     1021 atctctcgca catcctgctt accagtggcc gttgccagtg gcgttaagtc gtgtcttacc
     1081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1141 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aaccgagata ccaacagcgt
     1201 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1261 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1321 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc ggtttttgtg atgctcgtca
     1381 ggggggcgga gcctatggaa aaacgccaac ggcgcggcct ttgtccctgg cttcggcttt
     1441 ttgctcacat gttctttccg gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1501 ttgagtgagc tgacaccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg
     1561 aggaagcgga agagcgcctt atgtgacatt ttctccttac gctcctctat gccgttctgc
     1621 atcctgtccg gatgcgttat atcccggtaa gattttccgc ttcaaagcgt gtctgtatgc
     1681 tgttctgaag ttcttccgcg agttcgtgca gcttctcaca catggcggcc tgttcgtcag
     1741 catcaagtgc gtccagtttt tcgagcagcg tcaggctctg gctttttatg aatcctgcca
     1801 tgttgagtac ggtttgctgc tgcttgttca tctttctgtt ttctccgttc tttctgtcat
     1861 ctgcgttgtg tgattatatc gcgtaccact tttcgactgt tttaccgtcg gtattctgct
     1921 gtctgactct ttgacgggca tttctgtcag acaacactgt cactgccaaa aaactgccgt
     1981 gcctttgtcg gtaattcgaa cttgctgaca ggacaggatg tgtaattgtt ataccgcgca
     2041 tacatgcacg ctattacaat taccctggtc agggctttgc cccgacaccc atgtcagata
     2101 cggagccatg ttttatgaca aaacgaagtg gaagtaatac gcgcaggcgg gctatcagtc
     2161 gccctgttcg tctgacggca gaagaagacc aggtaatcag aaaaagggct gctgaatgcg
     2221 gcaagaccgt ttccggtttt ttacgggcgg cagctctcgg taagaaagtt aactcactga
     2281 ctgatgatcg ggtactgaaa gaagttatga gactgggggc gttacagaaa aaactcttta
     2341 tcgacggcaa gcgtgtcggg gacagggagt atgcggaggt gctgatcgct attacggagt
     2401 atcaccgtgc cctgttatcc aggcttatgg cagattagct tcccggagag aaaactgtcg
     2461 aaaactgacg gtatgaacac cgtaagctcc caaagtgatc accattcgct ttcatgcata
     2521 gctatgcagc gagctgaaaa cgatcctgac gcatccttcc tgtttttccg gggtaaaaca
     2581 tctctttttg cggtgtctgc gtcagaatcg cgttcagcgc gtttcagtgg tgcgtacaat
     2641 taagggatta tggtaaatat atgagctatg cgataacttt aactgtgaag cgatgaaccc
     2701 attacaggca aagccaatta ctcctgacag tggtttagcc agaagcaggg ctaccaagac
     2761 caatgcaata agtaatatat cgttttgcta tcgtgcatcg ctcgcgctca gttccattgt
     2821 gcttttttaa gctgtcgttt ttcttacggt atataccggt tttttatggc gtggtttctt
     2881 aacttgttca gctactgatg gacccattgt atctaggtag tcaactagct ttgttagatc
     2941 ataaatattg cgaccaccat atcggcgatc actcttcgat gttggtttct tgtccaagag
     3001 attagctttt tcaagatcat tgatagctct ctgaacagtc cgtacagaaa cccccatacg
     3061 tatggctaga ctttccattg acggatgcgg ccactcttgc aaactccacc agtgaacgat
     3121 caggttaagt agtgtgttaa aggccactga agttagcttt ttctcgtttt gtat
//
LOCUS       DAFNBJ010000285         3168 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.286, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000285 DAFNBJ010000000
VERSION     DAFNBJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.286"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            150..1670
                     /locus_tag="L2X49_004848"
     CDS             150..1670
                     /locus_tag="L2X49_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBN4799790.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1695..2033)
                     /gene="maoP"
                     /locus_tag="L2X49_004849"
     CDS             complement(1695..2033)
                     /gene="maoP"
                     /locus_tag="L2X49_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBN4799791.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2152..2991
                     /gene="hdfR"
                     /locus_tag="L2X49_004850"
     CDS             2152..2991
                     /gene="hdfR"
                     /locus_tag="L2X49_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBN4799792.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3087..3162)
                     /locus_tag="L2X49_004851"
     tRNA            complement(3087..3162)
                     /locus_tag="L2X49_004851"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3127..3129),aa:Trp,seq:cca)
BASE COUNT          855 a          770 c          780 g          763 t
ORIGIN      
        1 gtccgctgca tttttgcgag catcattcca ccgccagaac aactcaactt cacttgcaca
       61 aataaacagg aatcagccac taaaattcac gaattagcgg gctacagcag cgatatctta
      121 tgcacataat ggtgactcaa ggagggctta tgtcactgtc aattgttcat acccgcgcag
      181 ccctgggagt aaatgcgccc ccgatcactg ttgaggtaca tatcagtaaa ggtctacccg
      241 gcttaacgat ggtgggctta ccagaaacaa cggtaaaaga agctcgcgat cgcgtgcgca
      301 gcgccattat caatagcgga tatgaatatc cggcgaaaaa aatcaccatc aacctggctc
      361 cagccgatct gccaaaagaa gggggacgat atgatttacc tatcgccatt gcgttgctgg
      421 cggcctcaga acagcttaca gccaataagt tagatgaata tgaattagtc ggagaactgg
      481 ctcttacagg cgctctgcgt ggcgttcccg gtgcaatctc cagtgcaact gaagctatta
      541 agtcgggcag aaaaattatc gtcgcgaaag ataacgaaga tgaagtgggg ctaattaacg
      601 gtgaaggatg cctgatagcc gatcatctgc aagctgtctg tgcgtttctg gaaggtaagc
      661 acgctctcga acgcccgaaa ccaactgatg cagtatcccg ggcgctacaa catgatctca
      721 gtgatgttgt cggtcaggaa caaggaaagc gaggactgga aattaccgcc gctggcgggc
      781 acaacctttt actgattggg ccgccgggaa caggtaaaac aatgctcgcc agccgtatta
      841 atggcctgtt gccagattta agcaatgaag aggcactgga gagcgctgcg atattaagtc
      901 tggtaaatgc tgaatcagta caaaaacaat ggcggcagcg cccgttccgc tcacctcatc
      961 acagtgcgtc gttaactgcg atggtgggcg gtggtgcaat tccagggcca ggtgaaattt
     1021 cgttggcgca taacggcgtg ctttttcttg atgagctacc tgaatttgaa cggcgtacac
     1081 tggatgcctt gcgagagccg attgaatccg ggcagatcca tctttcacgc acacgagcaa
     1141 aaataaccta tccagcccgt ttccagcttg ttgcggcgat gaatcccagc cctaccggac
     1201 attatcaggg aaaccataac cgctgcacgc cagaacagac attacgttat ctcaaccggc
     1261 tctcggggcc ctttctcgac cgcttcgatc tctcactgga gatcccatta ccaccccccg
     1321 gcattttgag taaaacggta gtgccgggag aaagcagcgc caccgttaaa caacgcgtaa
     1381 tggccgccag agagcgccaa tttaagcggc agaataaact gaatgcctgg ctggatagtc
     1441 cggaaatacg ccaattctgc aaacttgaga gcgaagatgc gatgtggttg gaaggaacac
     1501 tgatccatct ggggttatcg attcgtgcct ggcagcggtt attgaaagtt gcacgaacca
     1561 ttgctgatat tgatcagtct gacattatca cacgtcagca tttgcaggag gcagttagct
     1621 atcgtgcgat tgaccgtttg ctcatccatc tgcaaaaact actgacataa aaaaagggca
     1681 tttcgccctt tttattaatc atcagaatcg gtgtagtctt cagcaccttc aacctgcggt
     1741 ttaccgccgg aaagggtgtg aaaacgtttt ggacgcttaa tacgcgtcat atacttggac
     1801 cacacgcgtt ctgcttctgt cactggctca cgttcgccac ggcatactgc tacgaagagt
     1861 ttctcttcct cggtaaccgg ctcgcgtttg ccaagatcca actcattgaa ggcataacca
     1921 tgacgctcaa gcagttgtgc ctctttgatg gtgaaatcac catgacgaga gaatccacgt
     1981 ggataatgtt tattgtcgaa atatcgatta gtcgtcgtaa agctttccgc catcctgcac
     2041 gctcctaatt ctttgaccga gctagttatg gcgcggagta ttagttacgc ttgacagagt
     2101 gtaaaacaaa acatttaaat cataacgaca aataattttg cggagagcac tgtggatacg
     2161 gaattgttaa aaactttcct ggaagttagc cgaacgcgtc actttggtcg agcggctgaa
     2221 tcgctctatc tgacccagtc agcagtgagc tttcgaatca gacaactgga aaatcaactg
     2281 ggtgtgaacc ttttcacccg ccacagaaac aatatccgtt taaccgctgc cggtgaaaaa
     2341 ctactgcctt atgcagaaac gctcatgagc acctggcagg ccgcccgtaa ggaggtggcg
     2401 catacctcac gacataacga gttttctatc ggtgccagcg cctcgttgtg ggaatgtatg
     2461 cttaatcagt ggctgggacg tttgtatcaa aatcaggatg cccatacagg cttacagttc
     2521 gaagcgcgaa ttgcccaacg gcagtctctg gtaaaacagc tgcatgaacg ccagcttgat
     2581 cttcttatta ccactgaagc gcccaaaatg gacgaattta gtagtcagtt gctgggatat
     2641 ttcactttag cgctttatac cagtgcccct tcaaaactaa agggagatct taattatctg
     2701 cgacttgagt gggggccaga ttttcaacag catgaggcag gtttgatcgg tgctgacgaa
     2761 gtgcccattc tgacaaccag ttctgctgaa ctggcacagc aacagattgc gatgcttaat
     2821 ggttgcacct ggctacccgt cagctgggcg cgtaaaaaag gcggcctgca taccgttgtc
     2881 gatagcacaa cactttcacg gccgctttat gccatatggc tgcaaaatag cgataaaaat
     2941 acgttgattc gcgatctttt gaaaattaac gtgctggatg aagtgtatta atatgaatgg
     3001 ctggcaagga tgccggtaga aggatttact tcggagaggg ttatttcaga taaaaaaaat
     3061 ccttagcttt cgctaaggat gatttctggc aggggcggag agactcgaac tcccaacacc
     3121 cggttttgga gaccggtgct ctaccaattg aactacgccc ctaattag
//
LOCUS       DAFNBJ010000286         3165 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.287, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000286 DAFNBJ010000000
VERSION     DAFNBJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.287"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            166..978
                     /locus_tag="L2X49_004852"
     CDS             166..978
                     /locus_tag="L2X49_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase NDM-7"
                     /protein_id="HBN4799793.1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQ
                     RFGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQIL
                     NWIKQEINLPVALAVVTHAHQDKMGGMNALHAAGIATYANALSNQLAPQEGLVAAQHS
                     LTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSL
                     GNLGDADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
     gene            982..1347
                     /gene="ble"
                     /locus_tag="L2X49_004853"
     CDS             982..1347
                     /gene="ble"
                     /locus_tag="L2X49_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin binding protein Ble-MBL"
                     /protein_id="HBN4799794.1"
                     /translation="MADHVTPNLPSRDFDVTEAFYAKLGFATSWKDRGWMILQRGGLQ
                     LEFFPYPDLDPATSSFGCCLRLDDLDAMVALVNAAGAEEKSTGWPRFKAPQLEASGLR
                     IGYLIDPDCTLVRLIQNPD"
     gene            1352..1990
                     /locus_tag="L2X49_004854"
     CDS             1352..1990
                     /locus_tag="L2X49_004854"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HBN4799795.1"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVA
                     GALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKP
                     VWKALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALP
                     WGLAGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
     gene            complement(2001..3032)
                     /locus_tag="L2X49_004855"
     CDS             complement(2001..3032)
                     /locus_tag="L2X49_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD N-terminal
                     domain-containing protein"
                     /protein_id="HBN4799796.1"
                     /translation="MTASTIRLRRWLAGLALLLALPATSAVAQDFELPPVDEVFVLSA
                     QATAPDRIEVRWRIADGYYLYRHRTSVKADAAFTGATMALPKGKAYRDEFFGDVETYR
                     KELLGTLTGTPAAGASATTLTVKYQGCADAGVCYPPQTRTLKVALPGEAGAGGFGCKA
                     RGLHDYVVKNGSADHPNAEVKFALGDVVNTMIGCTNGETIMLCHDTSLPRPYSLGFRV
                     QGTEGLWMDVNKSIYLEGKSPQPHRWEPAEGWFAKYDHPLWKRYADLAAGAGHGGMDW
                     FVIHAFVEALKAKAPMPIDIYDALAWSAITPLSEQSIAEGNRTLDFPDFTRGQWRTRK
                     PIFALNDAY"
     gene            complement(3037..>3165)
                     /gene="cutA"
                     /locus_tag="L2X49_004856"
     CDS             complement(3037..>3165)
                     /gene="cutA"
                     /locus_tag="L2X49_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBN4799797.1"
                     /translation="PALQERLRQLHPYELPELLAVEAASGLPEYLQWLAAESRPVN"
BASE COUNT          570 a          964 c         1021 g          610 t
ORIGIN      
        1 tagagaaatt tgctcagctt gttgattatc atatggcttt tgaaactgtc gcacctcatg
       61 tttgaattcg ccccatattt ttgctacagt gaaccaaatt aagatcatct atttactagg
      121 cctcgcattt gcggggtttt taatgctgaa taaaaggaaa acttgatgga attgcccaat
      181 attatgcacc cggtcgcgaa gctgagcacc gcattagccg ctgcattgat gctgagcggg
      241 tgcatgcccg gtgaaatccg cccgacgatt ggccagcaaa tggaaactgg cgaccaacgg
      301 tttggcgatc tggttttccg ccagctcgca ccgaatgtct ggcagcacac ttcctatctc
      361 gacatgccgg gtttcggggc agtcgcttcc aacggtttga tcgtcaggga tggcggccgc
      421 gtgctggtgg tcgataccgc ctggaccgat gaccagaccg cccagatcct caactggatc
      481 aagcaggaga tcaacctgcc ggtcgcgctg gcggtggtga ctcacgcgca tcaggacaag
      541 atgggcggta tgaacgcgct gcatgcggcg gggattgcga cttatgccaa tgcgttgtcg
      601 aaccagcttg ccccgcaaga ggggctggtt gcggcgcaac acagcctgac tttcgccgcc
      661 aatggctggg tcgaaccagc aaccgcgccc aactttggcc cgctcaaggt attttacccc
      721 ggccccggcc acaccagtga caatatcacc gttgggatcg acggcaccga catcgctttt
      781 ggtggctgcc tgatcaagga cagcaaggcc aagtcgctcg gcaatctcgg tgatgccgac
      841 actgagcact acgccgcgtc agcgcgcgcg tttggtgcgg cgttccccaa ggccagcatg
      901 atcgtgatga gccattccgc ccccgatagc cgcgccgcaa tcactcatac ggcccgcatg
      961 gccgacaagc tgcgctgagc catggctgac cacgtcaccc ccaatctgcc atcgcgcgat
     1021 ttcgatgtga cagaggcgtt ttatgcgaag ctgggctttg cgacgagttg gaaggatcgc
     1081 ggctggatga tcctgcagcg cggcggtttg cagctcgaat tcttccccta tcctgacctc
     1141 gacccagcta cgagctcgtt cggctgttgc ctgcggttgg atgatctcga tgccatggtg
     1201 gcattggtga acgcggcggg agccgaggaa aaaagcaccg gctggccgcg cttcaaagct
     1261 ccgcaactgg aggcgagcgg cctgaggatc ggctacctga tcgatcccga ctgcacgctg
     1321 gtgcggctga tccagaaccc cgactgaccg catgcccgcg aaaatcaaga tttgcgggat
     1381 cagcacaccc gaggcgctcg atgcgaccat cgcggcgcgg gcggactatg ccgggttggt
     1441 gttctatcca gcgtcgcccc gtgcggttac gtcgaatgtc gcgggcgctt tgacatcgcg
     1501 cgcagctggc cagatcgcca tggtcggttt gttcgtcgat gcggatgatg ctgtcatcgc
     1561 cgacgcactg gtggcagcca agctgaacgc gctgcagctg cacggttcgg aatcgcccga
     1621 acgcgtggcc cagttgcgcg cgcggtttgg caagccggtg tggaaggcgc tgcccgtcgc
     1681 cagcgccagc gatgtcgcac gcgccgcagc ctatgccggg gcggcggact tgatcttgtt
     1741 cgacgccaag acccccaaag gcgcgctgcc cggcggcatg gggttggcgt tcgactggtc
     1801 gctgctggcc ggatatcgcg gtgccttgcc gtgggggctg gcaggcgggc taaatccgac
     1861 gaatgttgcc gaggcgattg cgcgcaccgg agcgccgctg gtcgatacct ccagcggcgt
     1921 cgaaagcgcg ccgggcgtca aggataccga caagattacc aatttcgcct ttgcggtgcg
     1981 cttggcctaa atcgcgtcga tcaataggcg tcgttcagcg caaagatcgg cttgcgggtg
     2041 cgccactgcc ctcgggtgaa gtcgggaaaa tctaacgtgc gattgccctc agcaatcgat
     2101 tgttccgaca gaggcgtgat cgcgctccag gccagcgcgt cgtaaatgtc gattggcatc
     2161 ggggccttgg ccttcagcgc ctcgacaaaa gcgtggatca cgaaccagtc catcccgcca
     2221 tgcccggccc ctgccgccag atcggcgtag cgtttccata gcgggtgatc gtatttcgca
     2281 aaccagccct cggcaggctc ccagcggtgc ggctgtgggc tcttgccctc cagatagatc
     2341 gacttgttga cgtccatcca cagcccctcg gtgccttgca cccgaaagcc gagagaatag
     2401 gggcgcggca gcgaggtgtc gtggcacagc atgatcgttt caccattagt gcagccgatc
     2461 atggtgttga ccacatcacc cagtgcgaat ttcacctcgg cgttgggatg atcggcagag
     2521 ccgttcttga cgacataatc atgcagcccg cgcgccttac agccgaagcc gccagcgccc
     2581 gcttcgcccg gcaacgcgac cttcagggtg cgggtctgcg gcgggtagca cacgccggca
     2641 tcggcgcagc cctggtactt cacggtcagg gtggtcgcgc tcgcgccggc cgcgggcgtg
     2701 ccggtgaggg tgccgagcaa ttccttgcgg taggtttcga cgtcgccgaa gaattcgtcg
     2761 cggtaggcct tgcccttcgg cagcgccatg gtcgcgccgg tgaaggcggc atcggccttg
     2821 accgaggtgc ggtgccggta caggtaatag ccgtcggcga tccgccagcg cacctcgatg
     2881 cggtccggcg cggtggcctg cgcggacagg acgaagacct cgtcgaccgg cggcagttcg
     2941 aagtcctggg cgacggccga ggtcgcgggc agcgcaagca gcagggcgag cccggccagc
     3001 cagcggcgca ggcggatcgt ggatgcggtc attggctcag tttaccggtc ggctctcggc
     3061 ggccagccat tgcaggtatt cgggcaggcc ggacgcggct tcgaccgcga gcagctccgg
     3121 gagttcgtag ggatgcagtt ggcgcaggcg ttcctgcagg gcggg
//
LOCUS       DAFNBJ010000287         3108 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.288, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000287 DAFNBJ010000000
VERSION     DAFNBJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.288"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(174..1334)
                     /locus_tag="L2X49_004857"
     CDS             complement(174..1334)
                     /locus_tag="L2X49_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBN4799798.1"
                     /translation="MGVYVDKEFRVKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERIN
                     RKGASKEVVVKISGGAITRQGVRNSIDYMSRESELPVMSESGQVWKGDEIQEAKEHMI
                     DRANDPQNVFDDKGKENKKVTQNIVFSPPVSAKVKPEDLLESVRKTMNKKYPNHRFVL
                     GYHNDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKMKGYDVKATHKQQ
                     HGLNQSIKDAHKTAPKRQKGVYEVVDVGYDHYQNDKTKPKQHFIKLKTLNKGVEKTYW
                     GADFGELTTRENVKKGDLVKLKKLGQKEVKIPALDKNGVQHGWKTAHRNEWQLENLGV
                     KGIDRISSSSKELVLNSAEMIKKQQLQMRNFSQIKQSMIQSEQKVKIGIRLG"
     gene            complement(1337..1882)
                     /locus_tag="L2X49_004858"
     CDS             complement(1337..1882)
                     /locus_tag="L2X49_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="HBN4799799.1"
                     /translation="MKKVQFRIEESQHDDLLDCLKTLYPDEPGLTVAKGMKLLANALL
                     KSKVKNEDVNVSKDNDDFIKTTMYLTGKQRTLIEKAAHRHGWNLSRECRYRIQTTLEN
                     ELDFFDQELLVMNRCRNAIDKIGRNFHYIIVNDNARVLDKDGFYQDAERLSSEIMSLK
                     SEFENYIMLCKGRTVSNKVEV"
     gene            complement(1877..1951)
                     /locus_tag="L2X49_004859"
                     /pseudo
     CDS             complement(1877..1951)
                     /locus_tag="L2X49_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
     gene            complement(2223..2480)
                     /locus_tag="L2X49_004860"
     CDS             complement(2223..2480)
                     /locus_tag="L2X49_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799800.1"
                     /translation="MKKQEFLDFISAEQRRGAVRFSLGFNSKGEIVLHWTNEAGLRVW
                     SILSGNRGKSPSRANRERMSNLRRWLHDARQGMEGDTPEAE"
     gene            complement(2477..2641)
                     /locus_tag="L2X49_004861"
     CDS             complement(2477..2641)
                     /locus_tag="L2X49_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799801.1"
                     /translation="MSERMLSAIQTVEKGGRPVFPLMPFSAFPEYMALLRKALEKKET
                     KALIEKQEVL"
     gene            complement(2716..3054)
                     /locus_tag="L2X49_004862"
     CDS             complement(2716..3054)
                     /locus_tag="L2X49_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799802.1"
                     /translation="MNTYVITFQALNTKKEKITCSAFVHSETLFLAVHSFEDKNRGRG
                     YVITSVSELLSEELTAKNSLKKNINFWFNECGLSKSEVINEVINWKNFAYTLKELEEA
                     KNEAIRELRS"
BASE COUNT          786 a          753 c          567 g         1002 t
ORIGIN      
        1 ttatatctag atacatatgc tatttgataa caaaaaagca gatcgattga tcaccatgcg
       61 cttattttat agtcaagtta actaaatttt ttaccgctag ttacttttta caaaaactct
      121 gctaacaaga taaataaatc gatcttaatc aataagttac cggagagtat gaattatcct
      181 aaacgaattc cgattttcac tttctgttcg ctttgaatca tcgactgttt tatttgcgag
      241 aaatttctca tctggagttg ctgtttctta atcatctccg cgctgttcag caccagctct
      301 ttactggatg aagagattct gtctatgccc ttaaccccca ggttctctag ctgccactca
      361 ttcctgtgtg cagtcttcca gccatgctgt acaccgttct tatcaagtgc agggattttc
      421 acctcctttt gtcctaactt cttcagctta acaagatcac ctttcttaac gttctcgcga
      481 gtggttaact caccaaaatc agccccccaa tatgttttct ccacgccctt gttcagtgtc
      541 ttaagcttta tgaaatgctg ctttggtttg gttttatcgt tctgataatg gtcgtagcca
      601 acatcaacaa cctcataaac gcccttctgt cgttttggcg ctgttttgtg cgcatcttta
      661 atagactggt taagtccatg ctgctgctta tgagtggctt taacgtcata gcccttcatc
      721 ttcagctctt cacaaaaacc tgtacgaatt tcccgtaaat cttttttcct gatatcagct
      781 cgtttaccgt cattatcacg aatacgaaaa acgacatgca cgtgtggatg ttcttttttg
      841 tcgttatgat agccaagcac aaaacgatga tttgggtact tcttgttcat cgttttcctg
      901 acagactcaa gcagatcttc aggttttacc tttgctgata caggcggtga aaacacaata
      961 ttttgggtta cttttttatt ttccttacct ttatcatcga aaacattttg agggtcatta
     1021 gcacgatcta tcatgtgctc tttagcctcc tgaatctcat cacctttcca tacctgaccg
     1081 ctttcactca tcactggcag ctctgactcc cggctcatat agtcaatact gtttcgaact
     1141 ccctgccggg taatcgcacc tcctgagatt ttgacaacaa cctccttact ggcacccttg
     1201 cggttgatac gttcctgaac gttgcgttga tagtttttac caccattttt aactttatgg
     1261 gcgaaagcag acttgcggcc agcttcagag gacttacgct taaccctgaa ttccttgtca
     1321 acataaacgc ccatgattac acctcaacct tgttagaaac agttcgccct ttgcataaca
     1381 taatgtagtt ttcaaactca cttttgagac tcattatctc tgaactcaac cgctcagcgt
     1441 cctgatagaa cccatcttta tcaagtactc tggcattatc attaacgata atataatgaa
     1501 agtttcgccc tattttatct attgcattac ggcaacgatt catcaccaga agttcctgat
     1561 cgaaaaaatc aagttcattt tcaagtgtcg tctggattcg ataacgacat tctctggata
     1621 aattccagcc atgacgatga gccgctttct ctattaaagt tctttgtttc cctgtcaaat
     1681 acattgtggt tttgataaaa tcatcattgt cttttgacac attaacatct tcatttttaa
     1741 ccttgctttt cagtaaagca ttggcaagca gcttcattcc ttttgccacg gttaaacccg
     1801 gttcatctgg ataaagtgtt ttaagacaat caagtagatc atcatgttgg ctttcttcaa
     1861 tcctgaattg aacttttttc ataaaactct ccaacaagaa ccgactgtag gtcaccgggc
     1921 aaacgttgcg gaatggcgtc agagacgtca ttttgcggcg tttgccctat cctgcatcgc
     1981 agtgaactac ctccacacat aaattgtgct tcagcgtcca tcgaaatgca aaaaatgaaa
     2041 ggctatcgtg gctgtgatct ggtgcgtctg cgaccggaga caacggattc gcggctaact
     2101 tgggcggaaa taaattcgct acgcgaatgg cctggcaggg ggcgcgagcg ctgttttacg
     2161 gaatatacaa aaaaagcacc tcccgtaaac gggagggctt cggcgattca ggaacgggaa
     2221 ttttattctg cctctggtgt gtcgccttcc atgccctgcc gggcatcatg gagccagcgg
     2281 cggaggttac tcattctttc ccggtttgcc cggctgggac tttttcccct gttaccgctt
     2341 agtatactcc agactcttaa cccggcctcg ttagtccagt gcaatacaat ttcccctttg
     2401 ctgttaaatc ccagcgaaaa ccggacagct ccccgcctct gttctgcact gataaaatct
     2461 aaaaactcct gttttttcat agcacctcct gtttttctat cagtgctttt gtttctttct
     2521 tttccagggc ttttctgagt aatgccatgt actcaggaaa agcagagaat ggcatcaagg
     2581 gaaaaaccgg acgcccaccc ttttcaacag tctgtattgc tgataacatg cgttccgaca
     2641 ttttatattc ctcgcgaggg aattgagtgt cacggtatca ggttgcctcc acaacctgat
     2701 accaccccta attgattaac tccgcagttc tcttatcgct tcatttttgg cttcctccag
     2761 ttctttaagg gtatatgcga aattcttcca gttaattact tcgtttataa cctcagattt
     2821 acttaaacca cactcattaa accaaaagtt aatatttttc ttgagggaat ttttcgccgt
     2881 cagttcctcg gacaacaatt cactaacaga cgtgataaca taaccacgac cgcgattttt
     2941 atcttcgaaa gaatgaacgg caaggaagag agtttctgaa tggacaaaag cagagcaggt
     3001 gattttttct ttcttagtgt ttaaagcctg gaaagtgata acatacgtgt tcattttagt
     3061 ctcccgcccg tttgggctac tggatcatgg taaggaacta ttcccgac
//
LOCUS       DAFNBJ010000288         3023 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.289, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000288 DAFNBJ010000000
VERSION     DAFNBJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.289"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(21..2714)
                     /gene="chiA"
                     /locus_tag="L2X49_004863"
     CDS             complement(21..2714)
                     /gene="chiA"
                     /locus_tag="L2X49_004863"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HBN4799803.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKSNDASNPWRYVRAATATEISETSNPQSCTSAPQPSPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEISQYGNPGSCSVKPDNNGGAVTPVDPTPETPVTPTPDNNEPSTPADSGND
                     YSLQTWSGQEGSEIYHVIFNGNVYKNAWWVGSKDCPRGTSAENSNNPWRLVRTATAAE
                     LSQYGNPTTCEIDNGGVIVADGFQASKAYSADSIVDYNDAHYKTSVDQDAWGFVPGGD
                     NPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPW
                     KPLGKAQSYSNEELNNAPQFNPETLYASDTLIRFNGVNYISQSKVQKVSPSDSNPWRV
                     FVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKD
                     ANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQH
                     YYDIVDNLNLKVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPIL
                     PTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIAN
                     LHSQLKGLHPNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLAMQDAQKRNLGMVG
                     IWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          760 a          768 c          687 g          808 t
ORIGIN      
        1 ggaaggtatc gccaacacat ttattgctta gtaaacggcg cgaagatttc gctaaatgcg
       61 tacttcggtg cctgttcttt agtcaggccg tggaattccg gagacaggtt agtgccgccc
      121 ggcaggtcgc gcgcgattga ccagatacca accataccga gattacgctt ctgcgcatcc
      181 tgcatggcca gacgagcatc agagagatag aacacctcgc cctgaacgtc gttcacgcca
      241 accatcggcg tggtacccat catagcgtca atttctgcat cgctcttatt gggatggagg
      301 cctttcaatt gtgaatgcag gttggcaatc gcagacgttg cacacttacc gtgaatgttc
      361 tggccttcgg tatttgcaga ctgacaaatc gcgttaccgt agtccattgt catcacgttc
      421 acacccgcca gctcaacacc tttcgctttg gcatcgctca ggacattcat cccttccggc
      481 gtcaggccag tcggcagaat tggcaaggtg taccagatag caatatcttt gccttctgac
      541 ttccatttat cctgcacttt cttcacagca aggttacgac gttcaataga tgcctgatcc
      601 gcaacccagg tgccttcgat atcgaagtcc aggactttga ggttcaggtt atcaacgatg
      661 tcataataat gctgcatcag atcgtctacg ttcttacagg aagcagccag cggagcgttg
      721 ttagcaccac cgatagacag catcacatcg ccgccagcct cacgcagagc tttgattttg
      781 ctgtactgag cgtaattctg cataccataa gcggtacccc atgtcggcag acaggtgttc
      841 gcatctttac tcaccacaaa cgccagcgtg aagtggttga cgttatgatt cttagccagc
      901 gcagccaaat ccgggatcgt attcagcgta aagtcaacat acggtgcata aacgtgtttc
      961 ggccatgctt tcttcggcgt acctacacgt tctttggttc cggtccagtc aacaaaaaca
     1021 cgccacgggt tgctgtcaga aggagaaact ttctgcactt tactctgaga aatgtagttc
     1081 acaccgttaa agcgaatcag cgtatcgctg gcataaagcg tttctggatt aaactgcggc
     1141 gcattattca gctcttcgtt gctatagctc tgagccttac ctaacggctt ccacgggcgg
     1201 ctattgctac cggtggcgtt ttggttcgca accagagcag ggttgtcact ttgcgtccag
     1261 aacagcgctt cataagcctg cccatcaaca acaacgcgat cacctttcac gtacacagtg
     1321 gatgcggacc atgctttcgc cggttcgtat ttcttccacg ggttatcgcc gcccgggaca
     1381 aagccccatg cgtcttgatc gacagaagtt ttataatgtg catcgttata atctacgatg
     1441 ctgtccgcgc tgtacgcttt gctggcctgg aaaccatccg caacaatgac gccgccgtta
     1501 tcaatttcac aggtagtcgg gttaccgtac tgactcaatt ccgcagcggt agctgtacgc
     1561 acgagacgcc atgggttatt ggagttttca gcgctggtac cccgtgggca atctttagac
     1621 ccaacccacc aggcgttctt gtaaacatta ccattgaaaa taacatggta aatttcgcta
     1681 ccttcctggc cgctccacgt ttgcaatgag taatcgttac cgctatccgc tggtgttgat
     1741 ggctcgttgt tatccggggt tggggtcacc ggtgtttccg gagttggatc aaccggagtc
     1801 acagcaccgc cattattatc cggcttaacg gaacaggagc cagggttacc atactggctg
     1861 atttctgttg ccgttgccgc gcgaacataa cgccatgggt tactggcatc gttttctttc
     1921 gcatcgcctg ggcaatttgc cgcgcctacc caccaggcgt tcttataaat gccgccgtta
     1981 tagataacgt accatgtaga agaaccttcc tgacctttcc aggctactac agcatagttg
     2041 tcgtttgact tatcagctgg ggctggctga acatcaggag ccggtttaac gtccggtgcc
     2101 ggtttcacat ccggtgaagg ctgtggtgct gaagtacagg actgtggatt actggtttct
     2161 gagatttccg ttgccgtagc ggcacgaaca taacgccatg ggttgctggc atcattgctt
     2221 ttcgcatcac ccggacagtt agaagaggct acccaccagg catttttata taccgcacca
     2281 ttaaagacga cataccaggt ctgaccgccc tgctgtttat tccaggcaac tacagaagaa
     2341 ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagccgg cgtattggat
     2401 gcaggcgtat ttgaatttga agaagatgag ctgccgctct tttcgcagga aagtgtattg
     2461 ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg gttagttgca
     2521 tcatttgctt tcgcttttcc cgggcaattt gtagaagaaa cccaccaggc attttcataa
     2581 attttgccat cgaaaataac ctgatattta ttaccacctt gttggttatt ccatgcttcc
     2641 attgccaatg ctggcagagc ggaacacacc agccccatac caatcataga tttagtaaat
     2701 atatttaatt tcattataat cccttgtgac gtaaaaactg caaaacaaaa gtcctacgca
     2761 ggaattattt acgttttacg agaacccttc aatatgaatt aaagtgcggc attaaaaata
     2821 agaaaaaagc ctgacaaatg aagaatttta aaaacagaaa cattcatatt taaaatatta
     2881 aattgaattg atattttaaa tatgaataat ttattcgttc tgacagtacg aattagatat
     2941 acagccaaca aatgcaaaaa gggcgccgaa gcgccctttt caattcaaaa ctaattaacg
     3001 tgtaattagc ccagaacttt agc
//
LOCUS       DAFNBJ010000289         3010 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.290, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000289 DAFNBJ010000000
VERSION     DAFNBJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.290"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(156..232)
                     /locus_tag="L2X49_004864"
     tRNA            complement(156..232)
                     /locus_tag="L2X49_004864"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Asp,seq:gtc)
     gene            complement(365..1096)
                     /gene="dnaQ"
                     /locus_tag="L2X49_004865"
     CDS             complement(365..1096)
                     /gene="dnaQ"
                     /locus_tag="L2X49_004865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBN4799804.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DDELAAHEARLDLVQKKGGSCLWRA"
     gene            1161..1628
                     /gene="rnhA"
                     /locus_tag="L2X49_004866"
     CDS             1161..1628
                     /gene="rnhA"
                     /locus_tag="L2X49_004866"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBN4799805.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1625..2347)
                     /locus_tag="L2X49_004867"
     CDS             complement(1625..2347)
                     /locus_tag="L2X49_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBN4799806.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2381..>3010
                     /gene="gloB"
                     /locus_tag="L2X49_004868"
     CDS             2381..>3010
                     /gene="gloB"
                     /locus_tag="L2X49_004868"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBN4799807.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNE"
BASE COUNT          820 a          725 c          709 g          756 t
ORIGIN      
        1 tcattattga attttaatga atgagacaac aagcgagaat cacgcactta cgccagagta
       61 aaatcattga acagagaaat ggtggaattc aaatacaaaa aaccgcaaaa ttaaaaatct
      121 tgcggctctc tgaactcatt ttcatgagtg aatagtggcg gaacggacgg gactcgaacc
      181 cgcgaccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct ccgcgttgtg
      241 ttccgttggg aacgaggcga atattacgga ttgcctcgac cttcgtcaac ggtttttctc
      301 atcttttgaa tcgtttgctg caaaaatcac ccaagtcgct atttttagcg ccttttacag
      361 gtatttatgc ccgccagagg cagcttccgc ccttcttctg caccagatca agacgggctt
      421 catgtgctgc aagctcgtca tctgtcgcaa aaacaacgcg taacttactt gcctgacgta
      481 caatgcgctg aattgttgct tcaccttgtt gctgttgtgt ctctccttcc atcgcaaaag
      541 ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc cgcaaggatc tgggcatcga
      601 gtaatgcccc gtgtagcgtt cgtttactgt tatctatttc gtagcgggca cataacgcat
      661 cgaggctgtt gcgcttacca ggaaacattt tcctcgccac cgcaaggcta tcggtgacct
      721 tacagaaggt attggtcttc ggaatatcgc gcttaagcaa cgaaaactcg tagtccataa
      781 agccgatatc gaacgctgcg ttatggatca ccaactccgc gccgcggata tagtccatga
      841 actcatcggc tacttcggca aacgtgggct tatcgagcaa aaattcatcg gcaataccat
      901 gtacgccaaa ggcttcagga tccaccagcc gatcgggttt gagataaaca tggaagttat
      961 tgcccgtcag gcgacggttc accacttcaa cggcaccaat ctcaatgatc ttgtggcctt
     1021 catagtgcgc accaatctgg ttcataccgg tggtttcggt atcgagaacg atctggcgtg
     1081 taattgcagt gctcatagcg gtcatttatg tcagacttgt cgttttacag ttcgattcaa
     1141 ttacaggaag tctaccagag atgcttaaac aggtagaaat tttcaccgat ggttcgtgtc
     1201 tgggcaatcc cggacctggg ggttatggcg ctattttacg ctatcgcgga cgcgagaaaa
     1261 cctttagcgc tggctacacc cgcaccacca acaaccgtat ggagttgatg gccgctattg
     1321 tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt gagtaccgac agccagtatg
     1381 tccgccaggg tatcacccag tggatccata actggaaaaa acgtggctgg aaaaccgcag
     1441 acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct tgatgctgca ttggggcagc
     1501 atcaaatcaa atgggaatgg gttaaaggcc atgccggaca cccggaaaac gaacgctgtg
     1561 atgaactggc tcgtgccgcg gcgatgaatc ccacactgga agatacaggc taccaagttg
     1621 aagtttaagc ctgtggttta cgacattgcc gggtggctcc aaccgcctgg cgaattcgtg
     1681 gcttgttttt actctgtttc atcggattta gcgttaaagg aatagtccgt ttccgggcaa
     1741 caataagttg taagcaacca agcgcaggaa tatgcgcatt caatagtttt cctccgtgtt
     1801 tgttccacgg gagaacgtgg aaacggctgg cgtgtagcac ttcaaaattc aacaaagaga
     1861 gccagtccag ctgccgcatc agagtaaaca tccggctgtt atagggcgag gttttgcgca
     1921 ataccggcac aagtttgcgt aatcccatta aactgatggg attgaagcca ctaatgacca
     1981 gccagccatc atcaatcaat acccgatcgg cttcacgcaa taaacgatgc ggatcggtgc
     2041 accacggcaa tgtatgtgcc agtagacaaa catcaacgga tttatcggca aaaggaagat
     2101 gaagtgggtc cgcctgtacc tggacgggca ttccttgcgc agaaacattc acttgatgag
     2161 aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt gccaatctta agcagatgaa
     2221 aaccatacat tttagtgaac cacgggttga gctggcgctc cagcgccttg cgataaagct
     2281 ctccccaggg caaatcgccc cagcaatcag gagccacgac agtttgaggg actcttgccg
     2341 gtttcatcac aaccttccgt ttcacactga gaggtaatct atgaatctta acagtattcc
     2401 cgcctttgat gacaattaca tctgggtttt gaatgatgaa gcaggtcgct gcctgattgt
     2461 cgatcccgga gacgcagagc cagtattaaa cgccattgcc gccaataact ggcaaccaga
     2521 ggccatattt ctcacccacc atcaccacga tcacgttggc ggcgtaaaag aactggtgga
     2581 aaagtttccg caaattgtgg tgtatggtcc acaagagaca caagataagg gaacaacaca
     2641 ggtagtcaaa gatggcgaaa ctgccttcgt tttagggcat gaatttagtg taattgccac
     2701 accgggtcac actttaggac atatctgtta cttcagcaaa ccttatctat tttgcggcga
     2761 cacgctgttt tctggtgggt gtggtcggtt gtttgaaggg acaccatcac aaatgtatca
     2821 atcacttaaa aagttaagtg cgttacctga cgatacattg gtatgttgtg ctcatgaata
     2881 tacgttatca aatatgaagt ttgctttgag tattcttccg cacgatttgt ccataaatga
     2941 ttattatcgt aaagttaagg agttacgggc aaaaaatcaa ataacactac ccgtaattct
     3001 gaaaaatgag
//
LOCUS       DAFNBJ010000290         3003 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.291, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000290 DAFNBJ010000000
VERSION     DAFNBJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.291"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            64..396
                     /gene="hdeA"
                     /locus_tag="L2X49_004869"
     CDS             64..396
                     /gene="hdeA"
                     /locus_tag="L2X49_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBN4799808.1"
                     /translation="MKKVLGFILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            512..838
                     /gene="hdeB"
                     /locus_tag="L2X49_004870"
     CDS             512..838
                     /gene="hdeB"
                     /locus_tag="L2X49_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBN4799809.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            902..1549
                     /locus_tag="L2X49_004871"
     CDS             902..1549
                     /locus_tag="L2X49_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBN4799810.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1591..2121)
                     /locus_tag="L2X49_004872"
     CDS             complement(1591..2121)
                     /locus_tag="L2X49_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4799811.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2277..2843)
                     /gene="slp"
                     /locus_tag="L2X49_004873"
     CDS             complement(2277..2843)
                     /gene="slp"
                     /locus_tag="L2X49_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBN4799812.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          867 a          594 c          663 g          879 t
ORIGIN      
        1 tataacctca gtgtcgaaat tgattcgtga cggctctttc actttatagt tgaggatatt
       61 acgatgaaaa aagtattagg ctttattctt ggtggtctgc ttcttctgcc agttgtaagc
      121 aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc ctggacctgt
      181 gaagatttcc tggctgtgga cgaatccttc cagccaactg cagttggttt tgctgaagcg
      241 ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat tgcaaccgta
      301 accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa agataaagtt
      361 aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt tacatcgtac
      421 ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc ctcctgttca
      481 tatataatct ctatattgaa tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt
      541 tatttttatg ggcgctgtag cggctttgtc actggtgaac gcacaatctg cgctggcagc
      601 caatgaatcc gctaaagata tgacctgcca ggaatttatt gatctgaatc caaaagcaat
      661 gaccccggtt gcatggtgga tgctgcatga agaaacagta tataaaggtg gcgataccgt
      721 tactttaaat gaaaccgatc tcactcaaat tcctaaagtg atcgaatact gtaagaaaaa
      781 cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg
      841 aggtacaagt aaaaaggagt agcaagttga gccatcttgc tgctcctttt tgcattttta
      901 tatgacagca gaatttatta tacgtcttat actcgcggca attgcctgtg gcgctattgg
      961 catggaaagg caaatgcgcg gcaaaggagc agggttacgc acacatgtat taattggcat
     1021 gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt gctgacgtgc tgtctttaga
     1081 tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat
     1141 cggtgcaggt aacattctcg ttcgtaacca aaatattgta ggtctgacga cggcagcgga
     1201 tatctgggtg accgccgcca taggtatggt tattggcagc ggtatgtacg aactgggtat
     1261 ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa ccttccgcct
     1321 gatgaacaaa aattaccatt tacagctgac attagtgaac ggtaatacgg tatcaatgct
     1381 cgactggttt aagcagcaaa aaataaaaac ggatttagtt tcgttgcagg aaaatgaaga
     1441 tcatgaagtg gtcgccattg atatccagct tcatgccaca acgtcgatag aagacctgtt
     1501 gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat ctgtgtggca
     1561 gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat gctgatggcg
     1621 taccagctca ttgatccgct taagattgag gatcatcatg atattgtgtt tatggcagta
     1681 gaacgttttt agcgatattt tcagtttctc cagaatatct tccgttgtca tttgattcgc
     1741 aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acattcgctt caggcacaga
     1801 gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc gcggaacaaa
     1861 ggtcaccggt actttcccca gacagcgttt aatattaaag ggggagaaaa taatcacatg
     1921 gacaggtttt aatcgttcaa tttgagtgag aaaattagaa tgaaatacat tattcattaa
     1981 tgtatcaata atgacgaagg cattctgatg caacattacg tcgatctctt cttcgttctg
     2041 tatatgaacg acattacctt tactcagaat gtttttcatc gcggtgaaga acatcgtatc
     2101 cctggtaatt ataagaaaca taaccgactc ctggtgcgga cattaacttt ataatcagtt
     2161 tgccaaaggt aattaacagg acagggctta taataataat agttagctta ctgtatatta
     2221 attttatgta atacatgatg cagatgctga gaaagcatct gcatctttcg gtgttgttat
     2281 ttgaccagct caggtgttac ctgactcacc gcattggtgt agtaaggcgc accccagccc
     2341 ggttccggcc agaatgcacc atagccgtaa tcccacaggt tataggtggt attaaccact
     2401 tctctcaaat gccacacctg gatgccctgc atattcactt ccaggaagtt atacgggact
     2461 ttattgataa agcctggttg ttcaccctga atggtaccga ggatggtaac aaagtgatta
     2521 cgatagttca ctggatcaag gaagccgctt tgtctggcga gcagtcggcc ctgatagttg
     2581 gcttcaatat caggcttcgc atagctatcc aacggtaata cagcgatttc taacaacgta
     2641 tccgttttgc cattgataac gttgataacc ttcccaccaa agcgcgcttg ttgaccaaca
     2701 tataaccccg gctggttatg aacagcaaca aaactttttt gaatatcagg ttggttattg
     2761 cctttgatat tttgcggaat tgaactacat gcggcaagca aaaatgaaag gctgaggatg
     2821 agtgcacctt ttgtcatgtt catgttacta tccttatcaa caaatattaa agccataata
     2881 gatatttctt tatagttttc atctgattct gaggtgatat gcctgatagt aacgtaaacc
     2941 tgtctgatgt ttatatccct tttgtttagt tcaattccat tgattttggg gcgtggcatg
     3001 agt
//
LOCUS       DAFNBJ010000291         2964 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.292, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000291 DAFNBJ010000000
VERSION     DAFNBJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.292"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(280..1311)
                     /locus_tag="L2X49_004874"
     CDS             complement(280..1311)
                     /locus_tag="L2X49_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element ISEc33 family transposase"
                     /protein_id="HBN4799813.1"
                     /translation="MPIIVPIPRGERRLMQKAIHKTRDKNHARRLTAMLMLHRGERVS
                     DVARTLCCARSSVGRWINWFTHSGIEGLKSLPAGRSRRWPFEHICTLLRELIKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLHAGTVRRWLPSAGLVWRRAAPTLRIRDPHKDEK
                     MAVIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFIALLKHLKATYRRAKTITLIVDNYIIHKSRETQRWLK
                     ANPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETASPFP
                     GGKHGQAKV"
     gene            complement(1467..2648)
                     /locus_tag="L2X49_004875"
     CDS             complement(1467..2648)
                     /locus_tag="L2X49_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBN4799814.1"
                     /translation="MKLFGISSRDSGMDAMFYIQASTKQQASFIFDFLATRLYAHCQL
                     IEPLNETIIPSLSRTTLSLQPWSQNSQTRTSEMQMVTHGLTLSGAVMVEHSRQIVDFC
                     DNLGLRLDAPTGKPGWTELINADCEQLNSPRLYPVLIQPGTEGQFQIYVPAPAVIDAD
                     TVIASVIDECFSDVTSYCLAGTPFPMTEKPVIRMEAHLSDAHTRVFELTFISYNLALS
                     ATVYDKNLRQVLMLCEDFGVTLQGRAGASCHAYMRNEYRRLAHVGVYSRSGAENDVRA
                     YSSDKEETLIRQLMTQATAGSALIPAWHGKQFNGVLMNANPSSATTGFIYLSPEFPQG
                     QFMMTAEVVKSSCTPRGISYIETVDGYRFIVTAYDTKAMHSLTEFLAVNEDYFTPYRW
                     E"
     gene            complement(2657..2953)
                     /locus_tag="L2X49_004876"
     CDS             complement(2657..2953)
                     /locus_tag="L2X49_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBN4799815.1"
                     /translation="MIHSFKDRRLEKFFRNGKSTADIPAELINAISCRLETLNNVKNE
                     RELLSGSLRYERLRMTANRYSSIRVNSKYRLFFEWNDGVHNVHLSAHDYKSLIH"
BASE COUNT          686 a          758 c          752 g          768 t
ORIGIN      
        1 gcagggcgct gaattaccgg gcatccattg cccggacagt ttattaaaaa aaactgataa
       61 tgccccgccg gagaattacg ccaattcctg tgaaggcatg gtctggctga aaagttactt
      121 ttcccggttg ccctgccttg atcccgtatc gtttataccg gtctgctctt acatcctacc
      181 tgggcagcag ctggctacgg tgatcgggta ttaaaataga accggtctgt tttaccgcag
      241 attcaacgcc ccgcacctaa atagctgcgc ctaataccgc tacacttttg cctgaccatg
      301 ttttcctccg gggaatgggc tggcggtttc cataaaatgg cggacctttt tcagtaactg
      361 ccacattgag cggcactgat gattacgggt tatcgtgtca tgaagtgcct gccatagccg
      421 ttccacatga ttcacccacg gcgagtaaac cggctggtaa attaccctga acttgggatt
      481 tgctttcaac cagcgctgtg tttcgcggct tttatggata atgtagttat caacgatcag
      541 cgtgattgtt ttcgcccgcc ggtaagtggc tttcaggtgc ttcagcagag cgataaacag
      601 cgctgaactt ttgctgttgc cgcccacgta gctgacttta cccgtgccac tgtgcagtgc
      661 gccggccaga tagtattttt cgttctgccc cggcgtcact acccgtttct gctgtccgcg
      721 caactgccag tccgcaccga ttttaggatt aaggtggata tccacttcat cttcataaaa
      781 taccggatgc tctgcgctgc attcatccag cgctttgtgg attaccgcca tcttttcatc
      841 tttatgtggg tcacggatac gcagagttgg cgcggccctg cgccatacaa gccccgcaga
      901 tggcaaccag cggcgaacgg ttcctgcatg taactggcaa ccggttatct cattgatttt
      961 tattgccagt aattcggtgc tccagcgtga acgttgataa ccaaaatcgc cgggagaatg
     1021 ctttatcagc tcacgtaaca gggtgcagat atgttcaaaa ggccagcgtc gggagcgccc
     1081 tgcgggtaag gatttcaggc cttcaatacc tgagtgcgta aaccagttaa tccagcgacc
     1141 aacggatgaa cgggcacaac agagagttct ggcaacatcg ctgacccgtt caccccgatg
     1201 aagcatcagc atggccgtga gtctgcgggc atgattttta tcacgcgttt tatgaatagc
     1261 tttctgcatc aggcgtcgtt cgccacgggg tattggtact atgatcggca tcgctcagtc
     1321 cggttggtga ttttggttgg tttggcgatt gatcagatcg cacaatccgg gctgagttcc
     1381 ctttcagtga tctactattc cgcgcagcta tttagcgtaa aatcgccttt cccggatgcc
     1441 ctccttagtc gtccggcacg atggcgctac tcccagcgat aaggggtaaa atagtcctcg
     1501 ttaacagcaa gaaattcggt cagggaatgc atcgccttcg tgtcatatgc cgtcacaata
     1561 aaacggtatc cgtccaccgt ttcgatgtag ctgatccccc gtggcgtgca actggatttc
     1621 acaacttccg ccgtcatcat aaactgccct tgcgggaatt ccggtgacag gtagataaag
     1681 ccagtggtgg ctgatgacgg gttggcattc atcagcacgc cgttaaattg ctttccgtgc
     1741 cacgccggaa tcaacgcgct ccccgctgtc gcctgagtca tcaactggcg gatcagggtt
     1801 tcctctttat ctgagctgta ggcgcgaaca tcattttcag ccccggagcg tgagtagacg
     1861 ccgacatgag caagacgtcg gtactcgtta cgcatatagg catgacagga tgcaccggct
     1921 cgcccctgaa gagtgacgcc aaagtcttca cataacatca gtacctggcg caggtttttg
     1981 tcatagaccg tggcagagag cgccaggtta tagctgatga atgtcagctc aaaaacccgg
     2041 gtatgtgcgt cactaaggtg tgcctccatg cggatcactg gtttctccgt cattggaaac
     2101 ggcgtaccgg ccaagcagta cgaggtaaca tcgctaaaac actcgtcgat cacactggca
     2161 atgaccgtat cggcgtcaat gacagccgga gctggaacat aaatctgaaa ctgtccttca
     2221 gtgcccggtt ggatcaggac ggggtataga cgcggggaat tgagttgttc gcagtcggca
     2281 ttgatcagtt ctgtccaacc gggttttccg gtgggagcat caaggcgaag acccaggtta
     2341 tcgcagaagt cgacgatctg gcgggagtgc tccaccatca ctgccccgga gagtgtcaga
     2401 ccgtgcgtaa ccatctgcat ctcactcgtc cgggtctggg agttctggga ccagggctgt
     2461 aatgaaagcg tcgtccgact taaggaggga atgatggttt cattcagagg ttcaataagc
     2521 tggcaatgcg catacagccg ggtggcgagg aagtcaaata tgaagcttgc ctgttgtttt
     2581 gtgctggcct gaatataaaa catcgcatcc atgcctgaat cccgggatga aataccgaaa
     2641 agtttcattt tttaccttaa tggatgagcg atttataatc atgcgccgac aggtggacgt
     2701 tatgtacgcc atcgttccac tcaaagaata gccgatattt tgaattaact cgaatggaag
     2761 aataccggtt tgcggtcatt cttaagcgtt cgtaccgcaa actaccagat aacaattcgc
     2821 gttcgttttt aacattattt aacgtttcca atctgcatga aatcgcatta atcagttcag
     2881 caggaatgtc tgccgtggat tttccattac ggaaaaactt ttccaatcgc ctgtctttaa
     2941 atgaatggat catattttcc tccg
//
LOCUS       DAFNBJ010000292         2891 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.293, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000292 DAFNBJ010000000
VERSION     DAFNBJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.293"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            42..1253
                     /gene="bluF"
                     /locus_tag="L2X49_004877"
     CDS             42..1253
                     /gene="bluF"
                     /locus_tag="L2X49_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HBN4799816.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1458..2189
                     /gene="bluR"
                     /locus_tag="L2X49_004878"
     CDS             1458..2189
                     /gene="bluR"
                     /locus_tag="L2X49_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HBN4799817.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2410..2814
                     /locus_tag="L2X49_004879"
     CDS             2410..2814
                     /locus_tag="L2X49_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBN4799818.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
BASE COUNT          849 a          615 c          627 g          800 t
ORIGIN      
        1 taccgtctta ctctcgcctc acccattacc ctggattgtt aatgcttacc acccttattt
       61 atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat cgaagaaatg gtttcgatag
      121 caaatcgcag gaacatgcag tctgacgtaa cagggatctt actgtttaat ggttctcatt
      181 ttttccagct tctggaaggt ccggaagaac aggttaaaat gatatatcgg gctatatgcc
      241 aggatccacg gcactataat attgttgagc tgctgtgcga ttacgcgcct gctcgccgtt
      301 ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca cgagcgagat gacgttttac
      361 aggccgtatt cgacaaaggc acatcaaaat ttcagctaac ttatgatgac agagcgctac
      421 aattttttcg tacttttgtc cttgcaaccg aacaatcaac ctatttcgag atccctgccg
      481 aagactcctg gctttttatc gctgacggat ctgataaaga acttgattcc tgtgcccttt
      541 caccaactat aaacgaccac tttgcctttc atcctattgt cgatccctta tcgcggcgga
      601 taatcgcttt tgaagccatt gtgcaaaaaa atgaagatag cccatcagcc atagcggttg
      661 ggcagcgtaa agacggggaa atctacacag cggatctcaa aagtaaggcg cttgcattca
      721 cgatggcaca cgcacttgag ctcggtgata aaatgatttc aatcaatcta ttacctatga
      781 ccctggttaa cgaacctgac gcagtctctt ttttacttaa tgaaataaag gccaatgctc
      841 tggtgcctga acaaatcatc gttgaattta ctgaaagtga agtcatatct cggtttgatg
      901 agtttgccga agcgattaaa tcgctaaagg ctgctggtat cagtgtagca attgatcatt
      961 ttggcgcagg ttttgctggt ttgttactcc tgtcacgctt ccagcctgac agaattaaaa
     1021 tcagtcagga attgattacc aatgttcata aaagtgggcc acggcaggca attattcagg
     1081 cgatcataaa atgctgtaca tcacttgaaa ttcaagtcag tgctatgggc gtggcaacac
     1141 cagaagagtg gatgtggctt gaatccgcag gaattgagat gtttcaggga gatctgtttg
     1201 ctaaagctaa attgaatggt atcccttcca tagcgtggcc agagaaaaaa taattatcag
     1261 cacattcttt cacatgattt cagtaaatca tcgtgaaaat gaatacgtta cgtttaaatt
     1321 taatccacat taaaaataat gtttcatcca atttagctga aatcttattt atactttgta
     1381 caattttagc gtatattgta caaagtatta ttgggtcgtg tacaggcgac ggagatttgt
     1441 gaccgcaagg aggaattgtg gcttattaca gcattggtga tgttgctgaa cgttgcggga
     1501 ttaatcctgt cactctccgg gcctggcaac gccgctacgg tttgttaaaa ccacagcgca
     1561 gtgaaggcgg acaccgactc tttgatgaag aagacataca acgcatcgaa gagatcaagc
     1621 gttggataag taatggcgtc cctgtaggca aagttaaagc attactggaa accaccagcc
     1681 aggatacgga agatgactgg agccgcctgc aagaagagat gatgtcaatt cttcgcatgg
     1741 ctaatcctgc caaactacgc gcgagaatta tttcactggg tcgagagtac ccagtagatc
     1801 aattgattaa tcatgtttac cttcctgttc gccagcgtct cgtgcttgat cacaacacct
     1861 cccgcattat gagcagtatg tttgacggcg cattaattga atacgcagca acctcgcttt
     1921 tcgaaatgcg ccgtaagccc ggtaaagaag ccattctgat ggcgtggaat gttgaagaga
     1981 gagcacgatt gtggctggaa gcatggcgtt tatcattgtc aggatggcac atttctgttc
     2041 ttgctgatcc cattgaatcg ccgcgcccgg aactgttccc gacgcaaaca ttgattgtct
     2101 ggacaggcat ggcaccaacg agaaggcaaa acgaactttt acaacattgg ggtgagcaag
     2161 gttataaagt catctttcat gccccctaat ttcaccttta tcccgcccct tagacagaat
     2221 gttataatct gaacccacaa cagcgtttgt accggagctg gtccgttgca aaatgctcac
     2281 cgggagaatt cagatattcc ctctatcaca ctctgtacat atcacagcca aaggacagac
     2341 cagattgaag tggtaaaacc gatcgtgcac actacgcagt ctcacaacta acaagaggtt
     2401 attatcctga tggatcaggt cgttgttttt caaaagatgt ttgagcaagt gagaaaagag
     2461 caaaacttct cctggtttta ttcagaatta aaacatcacc gtattgcaca ttacatttat
     2521 tatctggcta cggataacat cagaattatt actcacgatg acacggtttt gttattaaga
     2581 ggaaccagga acctgttaaa agttagtacg accaagaacc ctgctaaaat aaaagaggcc
     2641 gcattgcttc atatttgcgg aaaatctaca tttcgggaat actgttcaac actagcaggc
     2701 gcgggcgttt tccggtgggt tactgatgtt aatcataaca aacgcagtta ctacgccatt
     2761 gataatacgc ttttatatat tgaagatgta gaaaataata aaccattaat ctagctaaag
     2821 ttggatgctt aagaaatgct tcataattca gtaaggcatt agcataatgg gaataaaagt
     2881 acagagacta t
//
LOCUS       DAFNBJ010000293         2850 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.294, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000293 DAFNBJ010000000
VERSION     DAFNBJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.294"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(162..1109)
                     /locus_tag="L2X49_004880"
     CDS             complement(162..1109)
                     /locus_tag="L2X49_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799819.1"
                     /translation="MASEHVQTIDLTNFSGRVFFLADPHGHYSTLCNLIHSISEPDEE
                     LIVFSTGNLFDYGPEPMELMTAINTGIFDGRSVRVFSAAGAGEEMLKKLLPINKDRRT
                     YYPSTFLNERWCARGGRWHKQINRNYLEEEICKLLSTQLATVMKVLFKGNISIGICPS
                     DYTDIRQGFNNTYNALLAFNQANVNIFQSQFLYGMEHAVRPVNVSDVNLVVLGRNPVN
                     SIRKAHGLPLTNLPVLIGNCLHINTGSLYMSEISSPALLAPGIPPTTVPAITLVELIL
                     ASTPTLICHQMILNKNGIYTQNTAPLNLDSNDNNIEATL"
     gene            1483..2517
                     /locus_tag="L2X49_004881"
     CDS             1483..2517
                     /locus_tag="L2X49_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stbA family protein"
                     /protein_id="HBN4799820.1"
                     /translation="MTELAKTKLAVAESNSTELETLKVVIDDGSKAAKLVCTNNQGDL
                     VPLLTQNSFVADFRVSHDGLIPFNYLIDGLQRFSHHSESSNALETTDVAHQYDEISRL
                     NVHHALHSSGLMPQDVHLYVTLPLSQFYTALGETNNENIQRKKDNLMKPVERYIDGKR
                     VSFNVVSVTVFPESLPAVTRADEIESIESFESSLVIDLGGTTLDVASITGQLEQISSV
                     RGFDRIGCSIVYDEIRRYLDSAKLNASNAYIHHLVDNRENKASLKVSPEDLDGVFNAV
                     NGAVAQLQEKVIKAVTQVEERPHNVFLVGGGSYLIEPAVRKHFDKSKIIMVDNPQFAL
                     SLAIADTVFA"
     gene            2577..>2850
                     /locus_tag="L2X49_004882"
     CDS             2577..>2850
                     /locus_tag="L2X49_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799821.1"
                     /translation="MTKKSEKENDRIQISAFWLSERQSPYAYNFLKKNALTHRGEQIS
                     LIRSAITTGLVLNNLFPELSSFINGLNERLTAADLNRFFNDEFNKDK"
BASE COUNT          810 a          544 c          631 g          865 t
ORIGIN      
        1 gttgacgtaa tttgcagcaa tagtaataaa ttcatagtta accccagttt tggcatcaga
       61 tagctgggac gacggtatcg gggttttagt cgatgcattt gtaatgttgg atggacctaa
      121 aaatgccagc aacggcaaaa ttatcagttc taatttaagc attataatgt ggcctctata
      181 ttattatcat ttgaatccag gttaagtggt gcagtgtttt gtgtgtaaat accgttttta
      241 ttcaggatca tttggtggca aatcagggtc ggtgtagatg ccaggatcaa ctcgaccagt
      301 gttattgccg gaactgtagt aggcggaata cctggtgcca gtagtgctgg gctactgatt
      361 tctgacatgt acagagaacc tgtattgatg tggaggcagt ttcctatgag taccggtaag
      421 ttagtcagcg gtagcccatg ggcctttctg atgctgttaa ctggattccg acccaatact
      481 acgaggttta catcgctgac attcactggc ctgactgcgt gctccatgcc gtatagaaac
      541 tgactctgaa aaatattcac atttgcctga ttaaaagcga ggagggcgtt atatgtattg
      601 ttgaagccct gccgtatgtc tgtgtaatcg gatgggcaaa tacctataga aatattgcct
      661 ttaaaaagca ccttcatcac agtagccagc tgggtggaga gcagtttgca gatttcctct
      721 tcaagatagt tgcggttaat ttgtttatgc catcgcccgc ctcttgcaca ccaccgttca
      781 ttaagaaatg tgcttggata ataagtcctg cggtctttat ttatgggtaa gagctttttc
      841 agcatctctt ctccggcccc ggcagctgag aacacacgaa cagaacggcc gtcaaaaatc
      901 cctgtgttga tcgctgtcat taactccata ggctcaggtc cataatcgaa gagatttcct
      961 gtggagaaaa cgatcaactc ctcatcaggt tctgatatcg aatggataag gttacatagc
     1021 gtcgaataat gaccatgagg gtctgccaga aagaaaaccc ttcctgaaaa attagtcaga
     1081 tcaattgtct ggacgtgctc ggatgccata aatgcctctt tctgaaattg cattaatact
     1141 accggatgaa agtcatcaat tttttcgata agaggggtaa tttttgtgag tgtccaaaat
     1201 ggagctagaa atcatttttc ggtttccgag ccgtatcaac tgtaatgcac ctcagttgtt
     1261 ttatttttgg tataaaaacg ctgagcaaaa tatagttttt gcgtgtgttg ctcaggtgta
     1321 catgaatcta gggttgtatc tggattaaaa ctgaaggatt cttttttaac aaggacttac
     1381 gttagttttt tgtcccgttt aatgttgacc taaagcaacc attggtgtat aaattccata
     1441 acaaaagtgg tgggctaaat aaaccaatac gagggtttct gcatgactga attagcaaaa
     1501 acaaaactgg ctgtggctga atcaaactct actgaacttg agacactcaa agttgttatt
     1561 gatgatggta gtaaagcggc aaaactggtt tgcactaaca atcaaggtga ccttgtacct
     1621 ttattgactc aaaatagttt cgttgcagat ttccgggtaa gccatgacgg acttattcca
     1681 ttcaattatc taattgatgg tcttcaacga ttctcgcacc acagcgaatc aagcaatgct
     1741 ttagaaacta ccgacgttgc gcatcagtac gatgaaatca gtcgtcttaa tgttcaccat
     1801 gctctacatt cctcaggtct aatgccacag gatgttcatc tctacgtaac tttgccactc
     1861 agtcagtttt ataccgctct gggtgaaacc aataatgaaa acatccaacg aaaaaaagac
     1921 aacctgatga agccggtgga gcgttatatt gatggtaaac gtgtttcatt taatgttgtt
     1981 tcagttaccg ttttcccgga atcccttcca gcggtaacgc gtgctgatga aatcgaatca
     2041 attgaatctt ttgaatcaag cctggttatc gatttaggtg ggacaacact tgatgttgca
     2101 agcattacag gtcagctgga acagatctcc agtgtcaggg gttttgaccg tattggttgc
     2161 tcaatcgtat atgatgaaat ccgtcgttat ttggattctg caaagctgaa tgcaagtaac
     2221 gcctatattc atcacctagt cgataatcgt gaaaacaaag cctcgctcaa ggtgtccccg
     2281 gaagatcttg acggcgtgtt caatgctgtt aatggggctg tggcgcaact tcaagaaaaa
     2341 gtgattaaag cagtcactca agtggaagaa cggccgcata atgttttcct tgttggtgga
     2401 ggttcttacc tgattgaacc ggcagttcgt aaacattttg acaaatcgaa aatcatcatg
     2461 gttgataatc cgcaattcgc actttcactg gctatcgcag atactgtttt tgcatgatga
     2521 tggttttgca tgatgatggt tttgcatgat gatggttttg caaaggggag agttttatga
     2581 ctaaaaaatc tgagaaggaa aacgatcgta ttcagattag tgcgttttgg ttatcggaac
     2641 gacaatctcc gtatgcatat aattttttga aaaaaaatgc cctaacacat cgtggcgagc
     2701 agatttctct aataagatct gcaatcacta cggggttagt gctaaataat ctcttccccg
     2761 agttatctag ttttattaat ggactgaatg agagattaac agctgctgat ctaaatcgat
     2821 tttttaatga tgaatttaat aaagacaaat
//
LOCUS       DAFNBJ010000294         2834 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.296, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000294 DAFNBJ010000000
VERSION     DAFNBJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.296"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            72..704
                     /gene="comR"
                     /locus_tag="L2X49_004883"
     CDS             72..704
                     /gene="comR"
                     /locus_tag="L2X49_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBN4799822.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(766..1305)
                     /locus_tag="L2X49_004884"
     CDS             complement(766..1305)
                     /locus_tag="L2X49_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBN4799823.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1532..2824)
                     /gene="ndh"
                     /locus_tag="L2X49_004885"
     CDS             complement(1532..2824)
                     /gene="ndh"
                     /locus_tag="L2X49_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBN4799824.1"
                     /translation="MKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWK
                     PLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKGEL
                     LVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYSAN
                     LGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILP
                     ALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPD
                     FLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQMA
                     TCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFV
                     YISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          677 a          777 c          699 g          681 t
ORIGIN      
        1 ctggactggt gaacggaggt gattattttt ataataacct ttcattcaca gtcaccaggt
       61 acaacggcat catggcaact gactcaacac aatgtgtaaa aaaaagccgt ggccgcccaa
      121 aagtgttcga cagggatgcc gcgcttgata aggccatgaa attgttctgg caacacggtt
      181 atgaagcgac ttctcttgcg gacctcgtcg aagcgaccgg agccaaagcg cccacgctat
      241 acgcggaatt taccaacaaa gaggggttat ttcgtgccgt tctcgaccgc tatatcgatc
      301 gttttgccgc taagcatgaa gcacagctgt tttgtgaaga gaaaagcgtg gagtctgcgc
      361 tggctgacta ttttgctgcc atcgccaact gctttaccag caaagacacc ccggcaggct
      421 gcttcatgat caacaactgc accaccctct ccccagattc aggagatatc gccaatacgt
      481 tgaaatcacg ccatgcgatg caagagcgca ctttgcagca gtttttatgt caacgacaag
      541 cgcgcgggga aatcccgacc cactgtgacg tgacacatct ggcagaattc cttaattgta
      601 ttattcaggg gatgtcgatc agcgcacgcg aaggtgcatc gctggaaaaa ctaatgcaga
      661 ttgccggaac gactttgcgt ttatggcccg aactggtgaa ataagtcgca aaaaaagccc
      721 ctcagcctga gcgaatgagg ggccaaattg cagagtacag ccttgttata ctttgttatt
      781 caaaatcagt tgcccattac tatctagcgg gatctgcgta cccggatcgc ggtccatgcg
      841 gattttgccc tgctgatcgc caatcttata ggtcacatca taaccgagca ttttttctga
      901 cttgtcatac accgttttac aacgctgttg cgtagtcgtg taagtatcgc tttcctggag
      961 agagccctgg atctggttac cggcatatcc accacccagc gcccccacaa cagtggcgac
     1021 atctttacca cgaccaccac caaactgatg ccctatcacg ccgccagcaa cagcgccgag
     1081 caccgacccg gtaatgcgat tttcatcctg caccggtcga cgatgggtca ctgtgacgtt
     1141 gcgacactcc tgacgcggtg ttttaaccgt ttccttgatt ggggttgcag aaacaacctg
     1201 agcgtattgc gggccccgtt caaacacgtt cagactggcc actgccgcta cgcccagcgc
     1261 agctgcgaca ccaatcccga tacccgccaa cattgattta ttcacgggaa cacctccttc
     1321 tttgcagtta tgccaatcat gcaagcgaag gagaaaagca gctatcagac agaagatgaa
     1381 aagcgcggga atggctggaa atacttaagg attcggagga gtctgaagga atgcctgatg
     1441 cgacgcttaa cgcgtcttat caggcctacc gctcgtacca tttgtccagg gcggatgagg
     1501 cgtttatgcc acatccgcca gtgtacgtcg attaatgcaa cttcaaacgc ggacggataa
     1561 cgcggttaat actccccacc agcatcatta atccggtttt aaagtaacca tgcagcgcaa
     1621 tctgatgcat tcggtatagc gagatatata caaagcgcgc aattcgtcct tcaatcatca
     1681 ttgagccgcg cgtcaggtta cccatcaggc taccgacggt ggagaagttc gacagcgata
     1741 ccagcgaacc atgatcttta tactgataat ttttcagcgg cttaccgttc atctgcgcca
     1801 gtatgttgtt cattgcgcaa gtcgccatct ggtgtgcagc ctgagcacgc ggcggaacaa
     1861 agcccccttc cggacgcggg catgacgcgc agtcgccaat agcgtaaatg tctggatcgc
     1921 gggtggtttg cagcgtcggt tccaccacca gctggttgat acggttagtt tcaagaccac
     1981 cgatatcttt caggaagtct ggcgctttga tcccggctgc ccacaccatc agatcagcct
     2041 caatatattc gccatcttta gtgtgcaggc cgccttcatc agcactggtg accatggttt
     2101 gcgtcagcac gcgaacgcca agtttcgtta gctcgttgtg ggccgcagca gagatacgtg
     2161 gcggtaatgc aggcaaaata cgttctcccg cttctaccag cgttacgttc agggcttcgt
     2221 tggtcaggcc tttgtaaccg tagctgtgca gttgcttgac cgcgttgtgc aattcagcgg
     2281 agagttctac acccgtcgcg ccgccgccga caatcgcaat gttcactttg ccatttgcgc
     2341 ccaggttggc ggagtatttc aggaagagat tcagcatctc ctggtgaaag cgacgcgcct
     2401 ggtgcgggtt atcgaggaaa atgcagttct ctttgacacc tggcgtattg aaatcgttag
     2461 aggtgctacc cagcgccatt accagggtgt cataggcgat tttacgttcc ggaaccagca
     2521 gttcaccttt ctcatcgcgc agttctgcaa tagtgattgt tttcgcttca cgatcaatat
     2581 caatgacgga acccagctgg aactggaaac catgattgcg cgcatgggcc agatagctca
     2641 acgcatcgac gccttcatca agcgagccag tcgccacttc gtgcagcagc ggtttccaca
     2701 ggtggctgtg gttacgatcg accagcgtaa ttttggcttt tttcttacgt cccagcttat
     2761 gccccagctg tgttgccatt tccagcccac cagcaccgcc gccgacaatc acaatctttt
     2821 tcaatggcgt agtc
//
LOCUS       DAFNBJ010000295         2799 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.298, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000295 DAFNBJ010000000
VERSION     DAFNBJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.298"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..150)
                     /locus_tag="L2X49_004886"
     CDS             complement(<1..150)
                     /locus_tag="L2X49_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBN4799825.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMNDSTLKEFIK
                     QYIAAS"
     gene            complement(202..1884)
                     /locus_tag="L2X49_004887"
     CDS             complement(202..1884)
                     /locus_tag="L2X49_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBN4799826.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            complement(2286..>2799)
                     /gene="ydeO"
                     /locus_tag="L2X49_004888"
     CDS             complement(2286..>2799)
                     /gene="ydeO"
                     /locus_tag="L2X49_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBN4799827.1"
                     /translation="VPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKGFITL
                     LTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQILLDA
                     RMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQSHTGMN
                     TGNTMSALAI"
BASE COUNT          774 a          557 c          606 g          862 t
ORIGIN      
        1 agacgctgcg atatattgtt tgatgaactc tttcagagtg ctgtcattca tccatttttc
       61 atgagtaaac tgcgactctt tttcaaggta aaaacagtaa tcacatttga gattacattg
      121 aaaactggag ggcttggctg taacgtgcat cgctatctcg ctcaataagg cggcggaaaa
      181 atccgccgca tgaaggttta gttatttcgc ttcgcttagt gctttcttga tattgttaaa
      241 cttctcctga tttacctcgc taagcggtgg ctggctgctg tcgataaact ctcttaccac
      301 gccttgcatc tctttaacga cctgcggatt ggcggcggca aggttatctt tttgctgtag
      361 atccgtcagt ttgtagagac ctaactgatt gttttctact gtatagacaa gcgaataatc
      421 gttatttctc accgtataag agaattggct taagtcctca gtgttggggt tatgcgggta
      481 atcgtctgac tgatggcgaa caaatttgtg gtaattatcc cagaatggaa tattttcctc
      541 gtcaaaccag tgagaataag aggttatcca ggtcagattt ttatgtggct cgccttgttt
      601 cttatcttgc aaccagggca gcaaggaaac gccatccagc ttaaggtctt ttggaatgct
      661 gatatcggct gcatcaagag ctgtcgggta gaaatccatt gcggaaatca gcttgtcata
      721 attaccgggt tgaagttttc ctttccacca cataaacatt ggggtgtgag taccgccagg
      781 ataggtctga ctcttatagc ctttttgcgc cccgttcagc ggcagaggac catcgataac
      841 cgcaccatta tcggaggtaa agagaataat tgtattgtca tactgtccgt ttttcttcag
      901 ttgttcgaga atgcgtttta caccctgatc aacagaataa acggaagcgt agtagttatc
      961 tgctgtttga ctaccggtat taaattgctt ctgatattga tccggtgcag gattatcatt
     1021 tggcaggtgc ggagcattat aagccaggta aagcataaaa ggctggtcaa gtgttttggc
     1081 acgatcaaca acgccaattg cctcatcggt taactgatcg ctgatataac cttttgcggg
     1141 gacacgttca cgatttttga acagtgaagg ggagttgtaa tatgccgttc ctgcagcgtg
     1201 gaatcccata aagtaatcaa agccacggtt ttgcggttgc cattcttccg cagaaaatgt
     1261 ggtgaagttg tcatgatagt cacgcgtttg tttatcttcc ggtaccggca cattactgat
     1321 ttttgacaag tgccatttac ctactgctgc agtgtaataa ccatgattct ggaataattc
     1381 aggcaagaaa gtttctgtta gcggaatacc atcctgagca tcggtattgg aatagacacc
     1441 aaagcgggcg ggagctcgac cggtcattat tgcggcacgg gaggggccgg aaacaccgtg
     1501 tgccacatag ccgttagtaa accgtacgcc ttcatccatt aatgaaagga gcgtcggcgt
     1561 tgatttttgt gcagcttcaa tggctttatc tatccctatt ttgtaggtat cgacaacttc
     1621 acgattttcc attgtttttg ggtcaaaaga tcccttatca aaaggaagtt gtccataacc
     1681 aagatcatcc atggttagta cgataatatt tggctttcct ttggtactgt attcagtcgg
     1741 cgtaaagtct gagaaagcaa cgtttgtttt ggttgctttc agctttacat catctgccgc
     1801 atgagcagca aatgcagcca taccagatgc cagtatcaaa gatatcgagg tacttacgac
     1861 acttttcttt aatgcagact tcataaatgt tcctcttctt attgtactta attccgaagt
     1921 aattttacga agagaaataa gtgggtgtaa gtgaagttag tcacataaag agatagcaga
     1981 tttagctaaa aaaagggaaa taacagtcca taaagcgttg acattacttt ctgttctatt
     2041 aagtaatttc tcgacgatag acaactaatt tattgatatt taataaatta ttgcatttta
     2101 ctgacaaaat gccgaattga gatcataaat aatcatgcaa caggttatgc aagtgcataa
     2161 atatgtgatg gatgtcactt atttatttca ataaatatat cgcctaaaaa caacgcgggg
     2221 cagggaatgg ctgccccata taattcttac gtagcgtgcg tggttgacta ctcgttagca
     2281 aataatcaaa tagctaaagc actcatcgtg ttgcccgtat tcatacccgt gtgactttga
     2341 caacggtact ccttagaaac tctcttcgga ctgttgccga aatgtttgcg gaacgcataa
     2401 ataaaataag atgtactggc ataaccacat tgttcggcaa ttttattgac tgaaccttct
     2461 acgcgtatca aattttttgc gtgctgcatt ctggcatcta atagaatctg tgagaatgtc
     2521 gtttgctctt gctttagttt tttcttcaac aggctttcac tgatgtacag gcagtcacaa
     2581 atatctttca gcttccatgg gtgcgccagc ttcatgttga caatatttct cacttttccg
     2641 gaaacggata gcacaccgtt agttaatagt gtaatgaaac ctttgcatgc ggcaaaaata
     2701 gacaggcaag aaaaaagcaa aagttcagaa aaattatgat gattgcgttc ttccgagttg
     2761 agataagcaa tcatttcatt aagcaatcca gtcggtaca
//
LOCUS       DAFNBJ010000296         2730 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.299, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000296 DAFNBJ010000000
VERSION     DAFNBJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.299"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..2081)
                     /locus_tag="L2X49_004889"
     CDS             complement(<1..2081)
                     /locus_tag="L2X49_004889"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBN4799828.1"
                     /translation="MRGWTTTCFMSTTLRTGSSAGPIQWQQHHKLSGIERAGTGAEGF
                     RYDRHGNLLAWTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVINPQ
                     GESYLYVLDNCGRVTEEHDWGGVVWRYRYDADGLYTAKVNGLEETILYSRDAAGRLAE
                     IITPEGKTQYAYDKSGRLTGIFSPDGISQRTGYDERGRVNVTTQGRRAIEYHYPDEHT
                     VIRCILPPEDERDRHPDESLLKTTYRYNAAGELAEVILPGDETLTFSRDEAGREVLRH
                     SNRGFACEQGWNAAGQPVSQRAGFFPEEATWGGLVPSLVREYRYDSAGNVSAVTSRED
                     YGRETRREYRLDRNGQVMAVTASGTGLGYGEGDESYGYDSCGYLKAQSAGRHRISEET
                     DQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQG
                     ELWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGFEL
                     ISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGLAL
                     SLPADTGYPDPRGELDPEAAPGLLYAGQWQDAESGLCYNRFRYYESETGMYLVSDPLG
                     LLGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTGFLDGVRLSRSQ
                     INNIAKEMEKLG"
     gene            complement(1993..2181)
                     /locus_tag="L2X49_004890"
     CDS             complement(1993..2181)
                     /locus_tag="L2X49_004890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799829.1"
                     /translation="MAGRCLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYE
                     YDAADRIIRWSDTVAAAP"
     gene            complement(2163..2366)
                     /locus_tag="L2X49_004891"
     CDS             complement(2163..2366)
                     /locus_tag="L2X49_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family domain
                     protein"
                     /protein_id="HBN4799830.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDA"
BASE COUNT          573 a          829 c          700 g          628 t
ORIGIN      
        1 cctagcttct ccatttcttt agcaatattg tttatttgac tacgtgataa acgaacccca
       61 tccaggaaac ccgtatgccc actaacacga cgaccatcgc caatatagtc catcaaactg
      121 ctgattttag aacttgcagc caatcccagc ggatcaaccc acccacacgg atttggcaca
      181 taccggtaag tctgctcacc ccccagcaac cccagcggat cactcaccag gtacatcccg
      241 gtttccgact cgtaataccg gaaccggtta tagcacagcc cggattccgc atcctgccac
      301 tgtcctgcat acagcaggcc gggggcggct tccgggtcca gttccccgcg cgggtccggg
      361 tagccggtgt ctgcgggcag gctgagtgcc agcccccaca ggctggtctg ccccggtcgc
      421 cagacggttt taccttcact gttaaagagc atcagcggac ggcccgtcag gtcgctcacc
      481 gcatgattcg tccgcgtcac ccactgcggg gccaccgacg gatgcggctg ccgtacccgg
      541 ctgaactgct ggcttatcag ctcaaagccg ttaaacacca ggtgccgtac gctgtacagt
      601 ttatcatcgc ggtattcccg gatttccgca atactgtcgc cgtcccacag atacgtgaag
      661 cggattttct tccggtcgca gcgtttctct gttcgtctgc cgctggcgtc gtggcggtat
      721 tcccacagct caccctgtgc gctgcaatac ccggtcagct ggtcacggct gtcccaccgg
      781 aaccgctctg tttccggtcg gtagccgtca cggtgtctgg tgcggctgac catccggcct
      841 gcggcgtcat agtcatactg cgtgtttccg gcctgtttca gccggtggcc tccggcatac
      901 tggtcagtct cttcacttat ccggtgcctg cccgcagact gcgccttcag gtagccgcag
      961 ctgtcatagc cataggactc atcgccttcg ccatagccca gcccggtgcc tgaggctgtc
     1021 accgccatga cctggccgtt ccggtccagc cggtactccc gccgtgtttc ccgtccgtaa
     1081 tcttcccggc tggtgacagc cgacacatta cccgcgctgt cgtaacggta ctcccgtacc
     1141 agtgagggaa ccagcccgcc ccatgtggct tcttccggaa aaaatccggc gcgctggctg
     1201 acaggctgac cggctgcatt ccagccctgt tcacaggcaa aaccccggtt actgtgccgg
     1261 agcacttcac gtcccgcctc atcacggctg aacgtcagcg tctcatcccc cggcaggata
     1321 acctctgcca gttctccggc ggcgttgtaa cggtaggtgg ttttcagcag ggattcatcg
     1381 ggatgtctgt cgcgttcatc ttccggtggc aggatacagc ggatgacggt gtgttcatcg
     1441 gggtagtggt attcaatggc ccgtcggccc tgagtggtga cattcacccg cccgcgttcg
     1501 tcatagccgg tgcgctgtga tatgccgtcc gggctgaaga tacccgtcag cctgccggat
     1561 ttgtcatacg catactgcgt tttgccttcc ggcgtgatga tttctgccag gcggcctgcg
     1621 gcatcccggc tgtagaggat ggtttcctcc agaccgttaa cctttgcggt atacaggcca
     1681 tcggcgtcat agcggtaacg ccagaccacg ccgccccagt catgctcttc cgtcacccgg
     1741 ccgcagttat ccagaacata aagatatgac tcaccctgcg ggttgatgac ttctgtcagt
     1801 tgcagcgtgt ctttatcgta gcggtactgc cagcggtggc cttcaccgtc cgttcgcgcc
     1861 accggcaaat cgaacggacc gtattccatc gtccagacaa cgccgttacc gtccgtccag
     1921 gccagcaggt tgccgtggcg gtcatagcgg aatccttccg cgccggttcc agcacgttca
     1981 atgccactca gtttatggtg ctgctgccac tgtatcggac cagcggatga tccggtccgc
     2041 agcgtcgtac tcataaaaca ggtggtagtc cagccgcgca tccgcttctg tcagccgccc
     2101 ccgcgcatcc tgttcgtagg tcgccagcgt ctgtatgccg ttatccgtgc ggtgaatggc
     2161 tttcaggcat cgccctgcca tatcgtcagg taggccattt tgtccagcac ctgcgcaaat
     2221 tcaagggaga gaaactgctg gctgaccagg gagagatcga gggaaaagag cgaagagagt
     2281 gactggttca gatgaaagga aaccaccgca aaagcatccg gcggcaggcc gtccacttcc
     2341 agtgtgaaac gtaatccggt tgacatagca acctccagta aaatgaataa tgatccgtac
     2401 cgtacaactg cggataatgt gataagcggg acgacttcag acgttattag ggaaggaaca
     2461 gaaaatgcta tggttatgtg agaattttgt ttaaatatta ctttacagaa acgggtgtct
     2521 gttcagtcaa aaaaataatc acaagctcaa cctcttgttt cttggcgtta ttcaggacag
     2581 gtacgcatat cacaacgagt aacaaaaacg taaagttaaa ccaatattca taatatattt
     2641 ttattcacag caataaataa tccaattaac attattaaaa tgaatgattg cataagaaat
     2701 atatgctacc gctccaccct gataacatga
//
LOCUS       DAFNBJ010000297         2586 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.300, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000297 DAFNBJ010000000
VERSION     DAFNBJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.300"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            433..576
                     /locus_tag="L2X49_004892"
     CDS             433..576
                     /locus_tag="L2X49_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBN4799831.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            589..798
                     /gene="cspE"
                     /locus_tag="L2X49_004893"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             589..798
                     /gene="cspE"
                     /locus_tag="L2X49_004893"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBN4799832.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            964..1773
                     /gene="rlmA"
                     /locus_tag="L2X49_004894"
     CDS             964..1773
                     /gene="rlmA"
                     /locus_tag="L2X49_004894"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBN4799833.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1770..2336)
                     /gene="mntP"
                     /locus_tag="L2X49_004895"
     CDS             complement(1770..2336)
                     /gene="mntP"
                     /locus_tag="L2X49_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBN4799834.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          677 a          608 c          620 g          681 t
ORIGIN      
        1 tctcttgatt ttttgctcat tcatcaattt ttcttatttt aaattttcaa tccctttggg
       61 gattgacttc tctttagggt aattgatagc cgttaactga ctgttttatg agaaaaagtg
      121 atataacttt ttattcattg catagcaaaa aatgtgatat tgcacgcact atgtaataac
      181 ttctcccact ggcctggaac aactgaactt attgaactat gttagaaact acgccagttt
      241 aagtatctgc ctgaactggc aaggttaagc acaatgatat atcggcgcgt attccgttgc
      301 ataagtgtgc aaaaaaagtg gaagacgtat cgagatttgt gcgtctgatc gagacatgtt
      361 taaaaatggc ttgccataat taacgttgta tgtgataaca gatttcgggt taaacgaggt
      421 acagttctgt ttatgtgtgg cattttcagt aaagaagtcc tgagtaaaca cgttgacgtt
      481 gaataccgct tctctgccga gccttatatt ggtgcctcat gcagtaatgt gtcagtttta
      541 tctatgttat gcctgcgggc gaagaaaaca atctaaggaa tttttcaaat ggcaaagatt
      601 aaaggtcagg ttaagtggtt caacgagtct aaaggttttg gcttcattac tccggctgat
      661 ggcagcaaag acgtgttcgt acacttctcc gctatccagg gtaatggctt caaaactctg
      721 gctgaaggtc agaacgttga gttcgaaatt caggacggcc agaaaggtcc ggcagctgtt
      781 aacgtaacag ctatctgatc gaatccactg atctgaagtg tgaatacgct tcaatctcgc
      841 tataaagcct cgtcgaatgc gaggcttttt actatgcttt atcttcgctc ctggcgttcg
      901 gatatttgcc cgccgcgtga ttcgcgttac acttgcggcc tttagtatcc tgccggagtt
      961 gtcatgtctt tttcctgtcc actttgccat cagcctcttt cgcgtgaaaa aaacagctat
     1021 atctgtcccc agcgacatca gtttgatatg gcgaaagaag ggtatgtcaa tctgctgccc
     1081 gttcagcata aacggtctcg tgatccgggc gacagcgcgg aaatgatgca agcacgccgc
     1141 gcattcttag atgccggaca ttatcagccg ctgcgtgatg caattgtcgc ccaactgagg
     1201 gaacggcttg atgataaggc cacggcggtg ctggatattg gctgtggtga agggtattac
     1261 acacacgcat ttgccgatgc gttgcccgaa atcaccacgt ttggtctgga tgtttcgaag
     1321 gtagcgataa aagcggcggc gaaacgctat ccgcaggtca ctttttgtgt cgcttccagc
     1381 caccgtttgc cgttttccga taccagtatg gacgccataa tacgtattta cgcgccgtgt
     1441 aaagcagaag aattagcacg agtagtgaag cccggcggct gggtcattac tgccacgccg
     1501 ggaccgcgac atttgatgga gctgaagggg ctgatttaca atgaagtaca tcttcatgca
     1561 cctcatgcag aacaactgga aggttttaca ttacagcaga gtgcggagtt gtgttatccg
     1621 atgcgtcttc gcggtgatga agccgtcgca ttattgcaga tgacgccgtt tgcctggcgt
     1681 gcgaagccag aagtctggca aacactggca gcaaaagaag tgttcgactg ccagacggac
     1741 tttaatattc acctctggca gcgttcttat taaccgtgga agtgcgtcca gaggatctgg
     1801 acgccgatgc cgatcagtac cagcccgccg agaatttccg cttttttccc aataattgag
     1861 ccgataaagc gaccaaccat catccctaat gttgacataa tcaaggttgc acaaccaatg
     1921 gccaatgcgg tcgcgataat gttgacctgc aggaaagcaa gaccaacacc cacagccatg
     1981 gcatccaggc tggtggcaat cgcggtggtt accagtagcc agaaaccgtg tcgacggcgc
     2041 ggctcttcat cttcatcatc tgcgccacga aaaccctcaa taatcattcg cccgccgagg
     2101 aatatcagca gcacaaacgc aatccagtgg ttccattcaa ggacaaaccg gctggctaac
     2161 atgcccattc cccagccgat cagcggcgtc agggtttcga cggcaccaaa aataaggccg
     2221 gttcgcaatg cttcagaaaa tttcggttta tggagggtgg cacctttacc gattgatgca
     2281 gcaaatgcat ccatcgacat accaaacgca agaagaacag tagcagtgat attcatgaca
     2341 atgtcctgac cggggtatcc ataaaacaca tcatcgcccc cagcaaacat gcatacatga
     2401 acaacaagcc gcgatggttt tcgcgttcat gcaggcatag cgattgatgt gcatatggtc
     2461 tcgcctgact gaaagtattc agtccgtacg tgccacgttt tgcaacgagt atgttgacac
     2521 gtacatttcc ggaatctgga aatcggctac tccccaaatg agggcgcaac cttaacatat
     2581 tttgag
//
LOCUS       DAFNBJ010000298         2531 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.301, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000298 DAFNBJ010000000
VERSION     DAFNBJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.301"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..912
                     /locus_tag="L2X49_004896"
                     /pseudo
     CDS             1..912
                     /locus_tag="L2X49_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEc9 family transposase"
     gene            complement(949..2156)
                     /locus_tag="L2X49_004897"
     CDS             complement(join(949..1835,1835..2156))
                     /locus_tag="L2X49_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBN4799835.1"
                     /translation="MTGILLGQEVRKRKTPQEKIAIIQQTMEPGMNVSHVARLHGIQP
                     SLLFKWKKQYQEGSLTAVAAGEEVVPASELTAALKQVRELQRLLGKKTMEVEILKEAV
                     EYGQSPKMDSARALVAKGRGIAMVSRTMGVSRAQLSLRINRSADWQDRRCNRRNEEAD
                     AEILSAILNIISDMPSYGYRRVWGILRKQRRTEGQPPVNAKRLYRIMSEHNLLLLHHK
                     PERPKREHKGKIAVAESDMRWCSDGFEFGCDNGEKLRVTFALDCCDREAIDWAASTGG
                     YDSSTVQDVMLRSVEKRFGDRLPDTAVQWLTDNGSAYTAYETRRFARELNLEPCTTAV
                     SSPQSNGMAERFVKTMKEDYIAFMPKPDVRTALRNLAVAFTHYNENHPHSALGYHSPR
                     EYRRQRTSLT"
     gene            2248..2531
                     /locus_tag="L2X49_004898"
                     /pseudo
     CDS             2248..2531
                     /locus_tag="L2X49_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          689 a          599 c          538 g          705 t
ORIGIN      
        1 aagaatctaa catcaaatgc aggtcttttt ctgctccttg agaatgcaaa aagcaatggg
       61 atttttgatt ttattgaaaa tgacctcgta tttgataatg actcaacaaa taaaatcaag
      121 atgaatcata taaagaccat gctctgcggt cacttcattg gcattgataa gttagaacgt
      181 ctaaagctac ttcaaaatga tcccctcgtc aacgagtttg atatttccgt aaaagaacct
      241 gaaacagtgt cacggtttct aggaaacttc aacttcaaga caacccaaat gtttagagac
      301 attaatttta aagtctttaa aaaactgctc actaaaagta aattgacatc cattacgatt
      361 gatattgata gtagtgtaat taacgtagaa ggtcatcaag aaggtgcgtc aaaaggatat
      421 aatcctaaga aactgggaaa ccgatgctac aatatccaat ttgcattttg cgacgaatta
      481 aaagcatatg ttaccggatt tgtaagaagt ggcaatactt acactgcaaa cggtgctgcg
      541 gaaatgatca aagaaattgt tgctaacatc aaatcagacg atttagaaat tttatttcga
      601 atggatagtg gctactttga tgaaaaaatt atcgaaacga tagaatctct tggatgcaaa
      661 tatttaatta aagccaaaag ttattctaca ctcacctcac aagcaacgaa ttcatcaatt
      721 gtattcgtta aaggagaaga aggtagagaa actacagaac tgtatacaaa attagttaaa
      781 tgggaaaaag acagaagatt tgtcgtatct cgcgtactga aaccagaaaa agaaagagca
      841 caattatcac ttttagaagg ttccgaatac gactactttt tctttgtaac aaatactacc
      901 ttgctttctg atgatcttga ccctccatct ccgaacagct tttgtatctt aagttaacga
      961 tgtccgctgc cgccggtatt ccctcgggga gtgatatccc agcgcgctgt gcgggtggtt
     1021 ttcattgtaa tgcgtgaacg ctactgcaag gtttcgcagt gctgttctca catccggttt
     1081 tggcatgaac gcgatatagt cttctttcat cgttttcacg aaccgttcgg ccatgccatt
     1141 actctgcggg ctgctcaccg ctgttgtgca gggctccaga ttcagctctc tcgcgaacct
     1201 ccgcgtttca tacgcggtat acgctgaacc gttatccgtc agccactgca ccgctgtgtc
     1261 gggcaacctg tcgccgaagc gcttttccac cgacctcagc atcacatcct gcacggtcga
     1321 actgtcatag cctcccgtgc ttgctgccca gtctatggcc tcacggtcgc agcagtccag
     1381 cgcgaacgtt acccgcagtt tttcgccgtt gtcgcagccg aactcgaagc catctgaaca
     1441 ccagcgcata tcgctttctg ccaccgctat cttgccctta tgttcacgct tcggtcgctc
     1501 tggtttgtga tgcaacaaca acaggttatg ctcgctcatt atcctgtaaa gccgtttggc
     1561 attcacaggt ggctgtccct ctgtgcgacg ttgcttgcgc aggatgcccc acacgcgtcg
     1621 ataaccataa ctcggcatat cgctgataat gttgaggata gccgacagta tttctgcgtc
     1681 tgcttcttca ttacgccggt tacagcgcct gtcctgccag tcggcagaac ggttaatccg
     1741 cagtgacagt tgcgcacgcg acacgcccat ggtccggctg accatggcta ttccccgtcc
     1801 tttggcaaca agggcgcgtg cgctatccat tttcgcgact gaccgtactc cacggcttct
     1861 ttcaggatct caacttccat cgttttcttg cccagaaggc gctgaagctc ccggacctgc
     1921 ttcagagcag cagtaagctc agaagcagga acgacttcct ctccagccgc aacggcggtg
     1981 aggctgcctt cctgatattg cttcttccac ttaaacagca ggctgggctg gataccatgc
     2041 aggcgggcga catgggagac attcataccc ggctccatcg tctgctggat aatggcgatc
     2101 ttctcctgag gagttttacg tttacggact tcttgcccta acaggatccc ggtcatctca
     2161 aaattggcgt tagtgttaga catatattca agcctatctc ttatctggag atacagctac
     2221 tgtccggtgt ttcagggggc tacatcatct gaaaaagtag ttatatacta tgaaaagcgt
     2281 ggtaatgctg aaaactatat caaagaagcc aaatacgaca tggcggtggg tcatctcttg
     2341 ctaaagtcat tttgggcgaa tgaagccgtg tttcaaatga tgatgctttc atataaccta
     2401 tttttgttgt tcaagtttga ttccttggac tcttcagaat acagacagca aataaagacc
     2461 tttcgtttga agtatgtatt tcttgcagca aaaataatca aaaccgcaag atatgtaatc
     2521 atgaagttgt c
//
LOCUS       DAFNBJ010000299         2528 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.302, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000299 DAFNBJ010000000
VERSION     DAFNBJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.302"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..738
                     /locus_tag="L2X49_004899"
     CDS             <1..738
                     /locus_tag="L2X49_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBN4799836.1"
                     /translation="QQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKLSYLR
                     RAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLARTLG
                     EQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHFSKAF
                     IGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVERFNR
                     VITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            1007..1225
                     /gene="osmB"
                     /locus_tag="L2X49_004900"
     CDS             1007..1225
                     /gene="osmB"
                     /locus_tag="L2X49_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBN4799837.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(1351..1677)
                     /gene="yciH"
                     /locus_tag="L2X49_004901"
     CDS             complement(1351..1677)
                     /gene="yciH"
                     /locus_tag="L2X49_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBN4799838.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(1677..2414)
                     /gene="pyrF"
                     /locus_tag="L2X49_004902"
     CDS             complement(1677..2414)
                     /gene="pyrF"
                     /locus_tag="L2X49_004902"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBN4799839.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
BASE COUNT          668 a          637 c          592 g          631 t
ORIGIN      
        1 caacaaacta ttctacagat ggtcattgac cagggtcagg ttagcgtaac cgatctggca
       61 aaagccactg gagtttctga agttaccatt cgccaggatc tcaacaccct cgaaaaactg
      121 agttacctcc gccgtgcaca tggctttgca gtttcgcttg atagtgatga cgtcgaaacc
      181 cgtatgatga gcaactatac gctgaagcgt gaactcgccg agtttgccgc gtcactggtt
      241 caaccgggcg aaaccatctt tatcgaaaat ggtagcagca atgccctgct cgcccggact
      301 ctgggggagc agaagaaaaa tgtcactatc atcacggtca gcagctacat cgcgcatttg
      361 ctgaaagacg cgccttgtga agtcattttg ctcggtggcg tgtaccagaa aaaaagcgaa
      421 agtatggttg gcccgttgac acgccagtgc atccagcagg tgcatttcag caaagcattt
      481 attggtattg atggctggca acctgaaact ggattcaccg gtcgcgatat gatgcgtacc
      541 gatgtggtca atgccgtgct ggaaaaagag tgcgaagcga tagtcctgac tgacagctcg
      601 aaatttggtg ctgtacattc atactccatc ggtcccgttg agcgattcaa tcgcgtgatt
      661 accgattcga aaatacgcgc cagcgatctg atgcatcttg agcacagtaa actcaccgtt
      721 cacgtcgttg acatttaacc atttcccttc tctacggatg atttgcagtt tggcaaatca
      781 tccgctctaa gatgattcct ggttgataat taagactatt tacctgttat taacacgctt
      841 aagatataaa attattatca gcgatataac aggaagtcat tatcacctgc gtgatataac
      901 cctgcgcgcg agcagatttc acggaataat ttcaccaggc ttattcttat ctattatagt
      961 tatagagagc ttacttccgt gaatcataaa ttcaggagag agtattatgt ttgtaacgag
     1021 caaaaaaatg accgcggctg ttctggcaat tactttggca atgtctctga gtgcctgttc
     1081 taactggtct aaacgggacc gcaacaccgc aatcggcgcg ggtgcagggg cattaggcgg
     1141 tgcagtactg accgatggca gtacgttggg tacattaggt ggtgcagctg tcggtggtgt
     1201 tattggtcac caggtcggta aataatcgta aataaatgtc gattatcgac tcgcaattat
     1261 aatacgcttg attctgttca gtacgaaata actgaataat acgcacagta aaatattcga
     1321 aagccacgga tatatccgtg gctttttatg ttaaccgcct gcgagtttta ccttcatccc
     1381 tttcgcttca agtagtgatt ttaataaatc acgcttatcg ccctggattt caataactcc
     1441 atctttaact gctccgccgc agccgcattt tttcttcagt tccgctgcaa gttttgtcag
     1501 ttcggcatca tcgagatcga caccggtaat caggcaaacg cccttacctt tacgtccact
     1561 ggtctgacgc tgaatacgca ccacaccgtc gcctttaggg cgtacagggg ccgctttggg
     1621 ctcatcaata cgtccggttt ccgttgagta gaccagacgg ctgttggaat cactcatcat
     1681 gcactccgct gtaaagaggc gttgatcgct ttcagcgtct gcgctggatc taccgattgc
     1741 gttaccgggc gaccaatcac catataatca acaccagccg acaacgcctg ttctggcgtc
     1801 ataatgcggc gctggtcacc agcttcactc ccctgcggac gaatgcccgg cgtaaccagt
     1861 ttgaactcct gaccgaatac ctgtttaaag cgcacagctt cctgagcaga acacaccaca
     1921 ccatcaaggc cacatttttg cgtcagtgcc gccagacgtt ctgcataatc tgcaggtgac
     1981 agtgtcatgc caagatcgac caggtcgctg gcttccatgc tggtcaacac tgtcacagca
     2041 atcaaaagcg gtgcatcttt gccaaacgga accagtgcct cacgcgctgc ggtcatcata
     2101 cgcgccccac cagaggcatg aacattcacc atccacacgc ctaagtcagc tgcagcagcg
     2161 acagcgtgcg cagcagtgtt aggaatatcg tggaatttca ggtcaagaaa gatatcaaaa
     2221 ccacgctgtt gaagttcgcg cacaaactgt ggcccaaaca atgtaaacat ctctttgccg
     2281 accttcagac gacaatcgcg tgggtcgatc ttgtcgacaa aggacagcgc gtcatcacga
     2341 ttatgataat caagggcaac aaccacagga gaattcgtaa cagcgcggga agaagatgaa
     2401 gcagttaacg tcatgaccag accttcttga tgatgggcac cggaagtggc gcgaaacagg
     2461 taaacggcga gcattctacc cgtggaaata aaaaattaac agttgcgatt tttcccctgg
     2521 caggcttt
//
LOCUS       DAFNBJ010000300         2464 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.304, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000300 DAFNBJ010000000
VERSION     DAFNBJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.304"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..2358
                     /locus_tag="L2X49_004903"
                     /pseudo
     CDS             1..2358
                     /locus_tag="L2X49_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275144.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
BASE COUNT          544 a          677 c          711 g          532 t
ORIGIN      
        1 gaaacattcc ttgaattacc ctcagaaatt caattttctg aggcggctaa cgctgccatt
       61 ctgacgcagg ataaatgggg cgatgtctgg cagacactga agaccgatgc ggatctgaat
      121 tatactgatg ccggtgagcc ggtcagtctc gcttcgcgcg tgacgacggc aaccagtgcc
      181 atttatcatg cgattaccga aggctggacc atgtgcgtcg gctacagcgg cgggaaagac
      241 tctcactcgc tactgcactt gttccttatg gccttgatcc gtgcagtacg aaacgggtcg
      301 aacgtcagcc agcaccactt cattcagatg tctgacaccc tgattgaaaa ccctgagatg
      361 catcatcagg ccacgcaggt tctcagccag ctcagagcct ttattgccga acaagaactg
      421 cctttgaccg tccttgtggc ccgcccgtcc atgacccaga gctgggtcgg acgcattctg
      481 accgggcgtg gacttccaac ctggacaaat tcttctacgc gtcaatgcgc tacagatcta
      541 aagcgttctc cactgcgcca ggccaaagcc cgttatctga aaaatgcacc tgcttctgtc
      601 cgcaacaaag tgtgcctgat gctgggttcc cgcgatgatg agagcgcccg tcgggcagac
      661 aacatcctaa gaatgggcgg gcaggcggac agcgtctcgc tcacggagca cggcggcgaa
      721 ctgtacccgg tgaaagagtg gcgggcacag gatatctggt catttctgat ggcctgtggt
      781 tctcaggcgc gatttccgct gccaagcttt ttgccggaca acttcagcct cgccacgctt
      841 tacaaagacg cgaccgggga atgtatctgg acgccggaga aatccactgt ccggtcttct
      901 gcctgcggcg ctcgctatgg atgctcgcta tgctgcgccg tcggcattga tcactcgatg
      961 gaaaccttac tccgcactga ccctgagaag tacggctacc aggctggtct caaccgcctt
     1021 cagcgtttcc tgaccaaaat tcagtacgac tggagccttc gggattatat cggacgcaaa
     1081 gtgtttgagg gcgggtatgt gcgtcttcag cccaatgtgt tctcgtcgtc cctgaccgaa
     1141 agactgttcc atatctgttg cagcctcgat tacgtggagg ctcagcgggc cgcagcggtg
     1201 catgaaaagt tactttcagg cgagatcgaa gataccgcgc ataaccgccg catgtcggag
     1261 ccgcagtttc gactggtgca ggaggctaac gtgctgcata tcgactttct ctggtcactg
     1321 cattgcttta acccgcgccc atttcgtgcc attgaacttt accgccaggt gtgggaaggg
     1381 ggggcactgg atcttctcga cgatgagcca gctatgcaac cagtaccccg cacgccgatg
     1441 ccagcaccac gctggatgaa gttgccggaa ggccgtatcg ggacgtcatt tgacgggctg
     1501 tccgatccgt catccgaaat agcctatttt gacggtcggg aggatgagcg agcttcccgc
     1561 agtctgatga gtagcgggga aggcctgaac atcgttgcat tcgaagaaga ggatgagctg
     1621 acggtcgatg aagacacggc atcctggatt atctggcatg aatatgacgg attacgccag
     1681 cgtgtgtctg acggtgagtt tacccctgtg gcggcggcac agtacctgct tagatacggc
     1741 gccgtccgaa tttcgcgggg gaaaggtgcg gtgtaccatc gactcgctta acgcgggcag
     1801 gcctattcac gtcttggcat taacgaccag acgccgctgc cgtctctgct cgccagccgc
     1861 cgtttcaaga tcctgaccga cagcgattat cgcttgctcg tagcccgcaa actgcggggg
     1921 cagcggcaga aactgcgctt ctggtgctgt gttgcggcct gtgtggcact gcacacctac
     1981 aacaaaaccc ctctgggggg gtggataacg acgcaactgg agacagaaca acagctacat
     2041 ctggcccgga cgggagacga actgaaggca aggactcttg acgctgtcct gaccctctgt
     2101 aacctgcgga tttcgtcaca gcaaccggaa gaacgtctgt attaccgggc cgtaagaaaa
     2161 cgctttctgg agacattagc cgggtgtatt tcgccggagc gcgaaggaac cttacgggaa
     2221 gtcatctggg agctgcggat gttatccggc ccccagtctt cgaaaaagac ggggttccgg
     2281 catgtggatg aatgccggga gaggacggcg gcgttgttaa ggcctctgtt gaaacgtatg
     2341 gtccgcctgc tggcgtgagc ccaccagcaa aatcagacat acgtgcaaaa aacgtgaaaa
     2401 tagccccgtt aacggaaaaa gcgccgaatg gtgtgtgttg taatgagcgc aggtgcggaa
     2461 ttcg
//
LOCUS       DAFNBJ010000301         2454 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.305, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000301 DAFNBJ010000000
VERSION     DAFNBJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.305"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            64..2331
                     /gene="adiA"
                     /locus_tag="L2X49_004904"
     CDS             64..2331
                     /gene="adiA"
                     /locus_tag="L2X49_004904"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBN4799840.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPCARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          621 a          669 c          608 g          556 t
ORIGIN      
        1 gcccgaaaag gccggaagat acttgcccgc aacgaagatt ccttcataac cgggtaagca
       61 atgatgaaag tattaattgt tgaaagcgag tttctccatc aagacacctg ggtcggtaac
      121 gccgttgagc gtctggcaga tgctttaagc cagcaaaatg ttaccgtgat taaatccacc
      181 tcctttgatg atggttttgc cattctctct tcaaacgaag ccattgactg cctgatgttc
      241 agctatcaaa tggaacatcc ggacgaacat caaaacgtca gacaattgat cggtaagctt
      301 catgagcgcc aacaaaacgt gccggtcttc ctgttgggcg atcgggaaaa agccctcgcc
      361 gcaatggacc gggatctgct ggagcttgtc gatgaattcg cctggattct ggaagatacc
      421 gccgacttta tcgccggacg cgccgttgcc gcgatgaccc gctaccgcca gcagctgttg
      481 ccgccactgt tcagcgcgct gatgaaatat agtgacatcc atgaatattc ctgggcagcg
      541 ccaggccacc agggcggcgt tggttttacc aaaacacccg ccggacgttt ctaccatgac
      601 tactatggtg aaaatctgtt ccgcaccgac atgggcatcg aacgaacttc cctcggttct
      661 ttgcttgacc atactggcgc atttggcgaa agcgaaaaat atgccgcacg cgtatttggt
      721 gccgatcgct cctggtcggt agtcgtcggt acttccggct ctaaccgcac catcatgcag
      781 gcttgcatga ccgataacga tgtcgtggtc gttgaccgta actgccataa atccatcgaa
      841 caaggtttga tgctgacagg cgcgaaaccg gtctatatgg tgccaagccg caaccgctac
      901 ggcattatcg ggccaatcta tccgcaggaa atgcaacctg aaaccttgca gaagaaaatc
      961 agtgaaagcc cgctgaccaa agacaaagcc gggcaaaaac cgtcttactg cgtggtgacc
     1021 aactgcacct atgacggcgt gtgttataac gctaaagaag cgcaggatct gctggaaaaa
     1081 acctccgatc gtctgcactt tgacgaagcc tggtacggct atgcacgttt caacccgatc
     1141 tatgccgatc actatgccat gcgcggcgaa cctggcgatc acaacggtcc taccgttttc
     1201 gccacccact ccacccacaa actgctgaat gcgctgtcac aggcttctta tattcatgta
     1261 cgtgaaggtc gtggggcgat taacttctcc cgcttcaacc aggcctacat gatgcatgcc
     1321 accacctccc cgctgtatgc catctgcgca tccaacgacg tggcggtgtc gatgatggac
     1381 ggcaacagcg gcctgtcact gacacaggaa gtgattgacg aagcggttga tttccgtcag
     1441 gcgatggcgc ggctatataa agagttcacc gctgacggta gctggttctt caaaccgtgg
     1501 aacaaagaag tcgtcaccga cccacaaacc ggcaaaacct atgactttgc tgacgcacca
     1561 accaaactgc tgaccaccgt tcaggactgc tgggtaatgc atccgggcga aagctggcac
     1621 ggcttcaaag atattccgga taactggagt atgctcgacc caattaaagt cagcatcctt
     1681 gctccgggaa tgggtgaaga tggtgaactg gaagaaaccg gtgttccggc ggcgctggtc
     1741 actgcctggc ttggtcgcca cggcattgtg cctacccgca ccactgactt ccaaattatg
     1801 ttcctgttct ctatgggcgt aacccgtggg aaatggggga ctctggttaa caccctttgc
     1861 tccttcaaac gccactatga cgccaacaca ccgctggcgc aggtgatgcc ggaacttgtt
     1921 gaacaatatc ctgacactta cgcgaacatg gggattcacg atctgggtga caccatgttt
     1981 gcctggctga aagaaaacaa cccttgcgca cggttgaacg aagcctattc cggcctgccg
     2041 atggcggaaa tcaccccgcg tgaagcgtac aacgcgattg tcgacaacaa tgtcgaactg
     2101 gtatccattg aaaatctgcc aggacgcatc gcggcaaact cagttatccc gtatccgcca
     2161 ggaatcccga tgctgctgtc tggtgaaaac ttcggcgata aaaacagtcc gcaagtaagt
     2221 tatttacgct cgctgcaatc ctgggaccac catttccctg gatttgaaca cgaaactgaa
     2281 gggactgaaa ttattgacgg tatttaccac gttatgtgcg tgaaagcgta accactattc
     2341 cgctgaaggc gtaattgttt aaataacatt acgccgcctg gccttaggcc ttttgagtat
     2401 ggcaacgttt tcataaaaat tgctgcaaac aaaaatgtca tactttttgc gcgg
//
LOCUS       DAFNBJ010000302         2414 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.306, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000302 DAFNBJ010000000
VERSION     DAFNBJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.306"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(181..1365)
                     /locus_tag="L2X49_004905"
     CDS             complement(181..1365)
                     /locus_tag="L2X49_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002001451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISEc29 family transposase"
                     /protein_id="HBN4799841.1"
                     /translation="MLTMNVKAMLADFLTFVTPKSMHKARFSVLLDAVTALAKDACCT
                     VTAIGRAMPGSSDKVSIKRADRLLNNPNLQRELPLIYAALTASIVGHKTKPMILVDWS
                     NADTAKRHFILRASIAADGRALTLLQKIAAAEDYTCPHLHGAFLKQLKAMLPKDCKPV
                     IVTDAGFKVPWLKQVRKLGWHYVARVRGNVKLKLAEQDKFISVNQLYRQAKKDPKSVG
                     KIMLAQTQHYETQAVLVGKGYKLLKRDKNKTYKEPWLLVSSLADCHGYADKIAKCYSS
                     RMQIEESFRDQKSHRYGLGSDLHGTKKKSRLEILLLLAALVNWFHYLLGSAAEKAGLH
                     LRYQANTVKNRRVLALNFLGILLCKEPKQRIRRQYYQQGLKQILQWVVQWDWAVIKQA
                     DS"
     gene            1414..1599
                     /locus_tag="L2X49_004906"
     CDS             1414..1599
                     /locus_tag="L2X49_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799842.1"
                     /translation="MFPNQLTFYFNYRISGDTSGGLQLFGLIGLIYGPIIFALTMVLL
                     RLYTIEFKDFLERQDNS"
     gene            1819..2100
                     /locus_tag="L2X49_004907"
     CDS             1819..2100
                     /locus_tag="L2X49_004907"
                     /inference="COORDINATES: protein motif:HMM:NF015042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799843.1"
                     /translation="MLYVVQGKDNPKLWKNIVSVSELHLINETSLLNNNYTASIRYRS
                     QDTPVKVTQNENGYIFEFSAPQWAPAVGQSLVLFQENECLGGGVISEIH"
     gene            complement(2081..>2414)
                     /locus_tag="L2X49_004908"
     CDS             complement(2081..>2414)
                     /locus_tag="L2X49_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (guanine(1405)-N(7))-methyltransferase
                     ArmA"
                     /protein_id="HBN4799844.1"
                     /translation="SIIGKLKTTIKYRFLNKESDVYKGTYDVVFLLKMLPVLKQQDVN
                     ILDFLQLFHTQNFVISFPIKSLSGKEKGMEENYQLWFESFTKGWIKILDSKVIGNELV
                     YITSGFQK"
BASE COUNT          697 a          537 c          460 g          720 t
ORIGIN      
        1 aagacacaac aaaagcccac cattataaaa tagtgtcact atgcaaatag ttgtgtctta
       61 acgaatgcgg atagaatgca taaacttacc taaaaaataa aattcagttt tatgataact
      121 ttactgttaa aagaagtcta gacaacctga gggatcccca caaaatttcc attcatacaa
      181 tcagctatca gcctgtttga ttactgccca gtcccactga accacccact gtagtatttg
      241 tttaagtccc tgctgataat attgcctgcg tattcgctgt ttgggttctt tgcaaagtaa
      301 tatcccaagg aaattcaggg ccaatacccg cctattttta acggtgttag cttgataacg
      361 caggtgcaaa cccgctttct ccgctgcgct acctagcagg taatgaaacc aattaaccaa
      421 tgcggccagt agtagcagta tctctaagcg agatttcttc ttggtaccat gcaaatcgct
      481 acccaggcca tagcggtgac ttttctgatc acggaagctt tcttcaattt gcattcggct
      541 actgtagcac ttagcaattt tatccgcata cccatggcag tcagctaagg atgacaccaa
      601 caaccatggt tccttatacg tcttattttt atcgcgtttc aatagcttat aacctttgcc
      661 aaccaggacg gcctgcgttt catagtgttg tgtttgggca agcatgattt ttcctacact
      721 ttttggatcc ttcttcgctt gccgataaag ctgattaaca ctgataaact tgtcctgctc
      781 tgccaattta agcttcacat tgcctcgaac tctagctaca taatgccatc ccagcttacg
      841 cacttgtttc agccatggaa ctttaaaccc cgcgtcagtg acaattacgg gcttacagtc
      901 cttgggtagc atggctttaa gctgctttaa aaacgccccg tgtaggtgtg ggcaggtata
      961 atcttctgcg gcggcaatct tctgtagcag agtcaacgct ctaccgtcag cggcaatgct
     1021 ggcacgcagg ataaagtgtc gcttggcagt atcggcattg ctccagtcaa ccaaaatcat
     1081 cggcttagtt ttatggccaa caatagaagc cgtcagcgca gcataaatca atggcagctc
     1141 tcgttgcagg ttaggattat tgagtagacg atctgcccgt ttgatactga ccttatctga
     1201 agagccaggc atcgcccggc caatcgcagt aacggtgcaa cacgcatcct ttgccagggc
     1261 ggttaccgca tctagcaaaa ccgaaaatcg ggctttatgc attgattttg gcgtgacaaa
     1321 ggtgaggaaa tcagcgagca tagcttttac attcatggtg agcatccgcg gtgtgggagt
     1381 tttggcgaat gatcagatca ggaaccgcca tgaatgttcc ccaatcaact aacattttat
     1441 tttaactaca gaatttctgg ggatacctca ggtggtttgc aactctttgg tttaattggt
     1501 ttgatttatg ggccaattat ttttgcgtta accatggtgc tgttacggct ttatactatt
     1561 gagtttaaag attttctgga acgccaagac aatagctaac aataagattg ttgtacttta
     1621 gcgcttcgcc ctgcccatat cggtatacag tgcatttacg ggagaaaaaa cggctcaaac
     1681 aggaaaaaat gaaatgactg agtcagccga gaagaatttc cccgcttatt cgcaccttcc
     1741 ctagattgct tttgagccat gcatgcattt gtttttaacc ttctaaactg tagttgaaac
     1801 ctatcttttc ctcaaaatat cctctatgtt gtccaaggaa aagataatcc aaaactttgg
     1861 aaaaacatag taagtgtttc agaattgcac ttgataaacg aaacttcatt acttaataat
     1921 aactatacag catctatccg ttatagaagt caggatacac cagtaaaagt aacccagaat
     1981 gaaaatggat atatatttga attttctgca ccacaatggg ctccagccgt tggacagagt
     2041 ttagtacttt tccaagaaaa tgagtgcctt ggaggcggag ttatttctga aatccactag
     2101 taatatatac taactcattc cctataacct tcgaatcaag gatttttatc caaccttttg
     2161 taaaagattc aaaccatagc tggtaattct cttccattcc cttctccttt ccagataaag
     2221 actttattgg aaaagatatt acaaagtttt gagtatgaaa aagctgtagg aaatccaaga
     2281 tatttacatc ttgctgtttt agcacaggaa gcatctttaa aaggaatact acatcataag
     2341 tacctttgta gacatcactc tctttattca aaaacctata ctttatcgtc gtctttaact
     2401 tcccaataat gcta
//
LOCUS       DAFNBJ010000303         2407 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.307, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000303 DAFNBJ010000000
VERSION     DAFNBJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.307"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            43..672
                     /locus_tag="L2X49_004909"
     CDS             43..672
                     /locus_tag="L2X49_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBN4799845.1"
                     /translation="MCEHLITVVEKYGAAERVHLGKQAGNVGRAVTKLPLMGKSLHKT
                     IERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGA
                     YDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRE
                     SLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(721..2220)
                     /gene="pitB"
                     /locus_tag="L2X49_004910"
     CDS             complement(721..2220)
                     /gene="pitB"
                     /locus_tag="L2X49_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBN4799846.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          652 a          604 c          578 g          573 t
ORIGIN      
        1 ttaattgccg ttattggtag cgatggttca ggcaagtcaa cggtgtgtga acatcttatt
       61 accgttgtcg aaaaatatgg tgctgccgaa agagttcatt taggaaaaca ggccggaaat
      121 gtcggtcgtg cagtgacaaa attaccgttg atgggaaaat ccttacataa aacaattgaa
      181 cgaaatcagg tgaaaacagc aaaaaaattg cctggaccag ttccggcgct ggtaattaca
      241 gcgtttgtcg cccgtcgctt actgcgcttt cgtcatatgc ttgcctgtcg tcgtcgcggg
      301 ttaattgttc taaccgaccg ttatcctcag gaccaaattc ctggcgctta cgatggtacg
      361 gtgttcccac ctaacgttga aggtggtcgt tttgtctcat ggctggcaag ccaggaacgt
      421 aaagcgtttc actggatggc gagccataag cctgatctgg tcatcaaact caatgttgac
      481 cttgaagttg cctgtgcacg taaacccgac cataaacggg aatcgctggc gaggaagatt
      541 gccataacgc cacagttaac ctttggtggt gcacaactgg ttgatatcga tgccaatcag
      601 ccactggaac aggtgttggt tgatgcagaa aaagcgatta cggattttat gaccgctcgt
      661 ggttatcact agtcaaaaat ggaaatgccc gatcgccagg accgggcatt ttcaggaagg
      721 ttaaatcaac tgcaatgcta tccagtacag cccaccagaa agaaaaattg ccgccggtaa
      781 agtaaatacc cacgccatca ggatgctggt taccgtttta cgctgtaacc caccgccgtc
      841 caccaccatc gtccctgcaa ctgcagacga gaggacgtgt gttgtggaga cgggcatccc
      901 aatataactg gcaagaccga tagacactgc cgccgtcatt tgtgccgcca tgccttgcgc
      961 atacgtcatg ccgcgcttac caatcttctc accgatggtc atcgctacac gacgccagcc
     1021 aatcatggtg ccaatgccga gcgccagtgc taccgccatg atgatccaca ccggagcgta
     1081 ctcaatggtg cttaacatat cgctgcgaag ttttttcagc aggttctggt cttctttact
     1141 gacgcctggc agtttcgcca gcttcgcgga ggtatcagag atgcacagca taatgcggcg
     1201 cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc ctggcagcat
     1261 cgttttaacg cgcgcaatcg catcaaaggt atttgccgga tgacagtgaa actctgttac
     1321 ttgcgtgcca tcagtcgatg ctgcaggcaa tggaggttcc atcgcaatca acttctgcgg
     1381 cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc gggtacgggt
     1441 aatttcatag ccggacgcat tcatattgac gacgaagcca gcaggggcaa tccccaccag
     1501 taccagcatt accaggccga tccctttttg tccgtcgttc gcgccgtgcg aaaacgccac
     1561 gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggtttac gtttgccttt
     1621 tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg tcccgctcca
     1681 gtagcgtcgc agcaggaata tcaggcctcc cgcaatgacc aggccgacga taggggaaac
     1741 aatcagcgag gagaaaattt tggtcacttc acgcaggttt aacgcatcca tcaccgatga
     1801 gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcaccaatca aggtgtgcga
     1861 actggaggcc ggtaaaccga agaaccacgt tcccaggttc cagataatcg ccgccagcag
     1921 catggaaaag accatcgcca ggccgtgggt tgaccccata ttcagcaaca aatcggttgg
     1981 caacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc caaaaaagtt
     2041 aaaaaatgcc gccatcacca cagcaagttg tggttgcatg gcacgagtat aaataacggt
     2101 tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca acacaaatgc
     2161 cagagcaagc aataacaaaa gccctgtgta tatatcaagg ccaacaaata aatttagcat
     2221 atattagatt acgccatttt gaatttacga acggacgcat tatcagtgac tttaacggca
     2281 tgggcaaagt gaaatatcat tttttaacga taaaaagagt gttatttctt gtgtatcaga
     2341 caataagcat tcattcaaaa cctttagaaa taatcaaaac acaggtattc cactggttaa
     2401 tatattc
//
LOCUS       DAFNBJ010000304         2391 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.308, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000304 DAFNBJ010000000
VERSION     DAFNBJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.308"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..598
                     /locus_tag="L2X49_004911"
     CDS             <1..598
                     /locus_tag="L2X49_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBN4799847.1"
                     /translation="SRSRCYIKTLIYKKYLRAFKRNTKINIFTELLIKSMAVRGFSLA
                     SIAEKNSLSEGAVSSVISSCYGLCSWRKKCKKDSLRRRHKQKILRFIHNQSVSITRKL
                     VKESCYASFYWLNKHECDWLNSCLPKTIRCYKNKRVDWSERDIISSSLINDVLSQGQY
                     SMSLTSLDALLGGHGWLLKYRDKLPMTMILLRKMELIK"
     gene            610..1536
                     /locus_tag="L2X49_004912"
     CDS             610..1536
                     /locus_tag="L2X49_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799848.1"
                     /translation="MVYENELWHSFLLRSQIMYNLSNCRNIISKHGALRYDAFPRFEL
                     IDMYKLHSLQDIYDILATRNGYILTSNIVSLSSGVYGYFNAIEDACRKNFHITNSYPL
                     VNISNIRYCHKCIVEDIHSKGIGYLRHRWLFESKCAVHSTSLYEVCFDNYLNAVKGLS
                     DLIISGINPHGYCSLVVDVSNPFKNPVYILPCARNKILKWISDNKNMLIYFLFDLFKC
                     KSHSSLSKVIEVKIMHDRYISAVFKMLSEVNFCNFNSFISDIFEDVSYASIGLDDYSV
                     NVHFLRLRAADCKFCQLNGKYFCSLYNVKLLR"
     gene            1889..2188
                     /locus_tag="L2X49_004913"
     CDS             1889..2188
                     /locus_tag="L2X49_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4799849.1"
                     /translation="MNTQYALKTLNQLRPVLIGFRKANGLTQKDVSERLGITQQTYAR
                     LEANPASAGFERLFRVFSVLGVEIVLSSREPLSDAKPEYGAMHNYSSLPARREKW"
BASE COUNT          723 a          414 c          468 g          786 t
ORIGIN      
        1 tagtcgcagc agatgttaca ttaaaacact tatttataaa aaatacctga gggcgtttaa
       61 gcgaaacaca aaaattaata tattcactga attgcttatc aagtctatgg ctgtaagggg
      121 gtttagtctg gcatccatag ctgagaaaaa ctcattatcg gaaggagctg tatcctctgt
      181 aatttcatct tgttacggtt tatgctcatg gcgtaaaaaa tgtaaaaaag attctttaag
      241 acggcgtcat aagcagaaaa tattaagatt tatacataat caatccgttt ctataacacg
      301 aaagttagtc aaagaaagtt gttatgcaag tttctattgg ctaaataaac atgaatgtga
      361 ctggcttaat tcctgtcttc ctaaaacaat acgatgctat aaaaataaaa gagtagactg
      421 gagcgagaga gatattatct catcatcatt aataaatgat gttttatctc aggggcagta
      481 ctcgatgtca cttacaagtc tagatgcttt actgggaggg catggctggc ttttgaaata
      541 cagagataaa ctaccaatga caatgatact tctcagaaaa atggaactaa ttaaataaga
      601 gggagttata tggtttacga aaatgaattg tggcacagtt ttcttctgcg ttcacagata
      661 atgtataact tatccaattg caggaacata atatcaaagc atggcgcact aaggtacgat
      721 gcctttcctc gtttcgaact gattgatatg tacaaattgc atagcttgca ggatatatat
      781 gacattcttg ccactagaaa tggttatata ctaacttcca atatagtgtc gttatcttct
      841 ggtgtttacg ggtattttaa cgctattgaa gatgcctgtc ggaaaaattt tcacataact
      901 aattcatatc cattggtaaa catctcaaac attcgttact gtcacaaatg tattgttgaa
      961 gatatacact ctaaaggcat tggttatctg cgtcatagat ggttgtttga atctaaatgt
     1021 gcagttcata gtaccagttt atatgaggtt tgttttgata actatttgaa tgcagtgaaa
     1081 ggacttagtg acttaattat aagtggaata aatcctcatg gttattgttc ccttgtggtt
     1141 gacgtttcaa accctttcaa aaatccagtg tatatattac catgtgcacg taataaaatt
     1201 ttaaaatgga tttcggacaa taaaaatatg ctgatttact ttctctttga tctcttcaaa
     1261 tgtaagtcgc attcaagttt atctaaggtt atcgaagtca agataatgca tgatagatat
     1321 atttctgcgg tgttcaagat gttaagtgaa gttaattttt gtaatttcaa ctcatttatt
     1381 tctgatattt ttgaggacgt aagttatgcc tctatcggac ttgatgatta ttctgttaat
     1441 gtgcattttc tcaggctaag ggctgcagat tgtaaatttt gccagttgaa tggtaaatat
     1501 ttttgctcac tttacaatgt aaaacttctt cgctaatttc acttcattta taaaatacta
     1561 agaaagctag ttagataagg ttttatattt tgactggggc aggaataaat tatcactcat
     1621 agagtttccc tgggggttac cacctgtgta tgatgccggt tagcaacaac gtctaaaaga
     1681 cctgcgatca cgtgggtccc tctccagcaa aggggcaagg ggtgctatat aacggtgtac
     1741 tgcttaccca atacggcctt gtatttgaaa gatgccgcat cttggcagga ttcgtattac
     1801 agcctatagt ctgttaatga gcattacagc ctatatgctg taaatccctg cgttatgctt
     1861 gttaactcgt cgatacggaa ggtcacttat gaacactcaa tacgctttga agactttgaa
     1921 tcagttacgt cctgttttaa tcggctttcg caaagccaat ggactgacgc aaaaagatgt
     1981 ttctgaacgt ttgggtataa cacagcaaac ttatgcccga ctagaggcca accctgcaag
     2041 tgcgggtttt gagcgtttgt ttagggtgtt cagcgtcctt ggagtggaga tcgtactttc
     2101 atccagggaa ccattgtcag atgcaaagcc cgaatacgga gctatgcaca attattcttc
     2161 acttccagct aggcgggaga aatggtgacg cctggcgctt cccggtctaa tttttcttca
     2221 acaagattaa ccccttagcg gcctgaagta tcaggttgcg atagagcctt tcatttcttc
     2281 tgttatgtta aatacaccat tttgagctat ttcgctcggc cttaaattat ctcttcattc
     2341 ttcagtgaaa attttatgtc gatcacatta tcgatgaatt taggaaataa t
//
LOCUS       DAFNBJ010000305         2373 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.309, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000305 DAFNBJ010000000
VERSION     DAFNBJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.309"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(286..1359)
                     /locus_tag="L2X49_004914"
     CDS             complement(286..1359)
                     /locus_tag="L2X49_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799850.1"
                     /translation="MRKSYTYGIPFGLHRDTGRFLDITEVSRGSACNCICPGCRTDLI
                     ARQGEVKLWHYSHSTDLLGDCDGLMEAIRGKIIEVIQEKQLLGFPHLLAGYDGGTVPL
                     DEVSGSGSMFGATADLFVKVNDLCIAVFLDIDRSVAGKLTFDHLHASEMVAALRIDLP
                     DIEYEISQVQLGRRDGTYSECIEKIIIDATESREWLYHPMMQELGTEPLKVYEGREPA
                     EAGPLLQRLSDSTLKLPDNLPQSMNVLIRMQQATIVCLCRLTVRASDIAQTDEFPLFM
                     RYFRLHCLDTSEHVNYEKLLNWQDMITKSKEGQLLTVEERDYLQEIVRIGLYNHRLKS
                     VCLTACEDNRVGESEGPKNYSLL"
     gene            complement(1639..2280)
                     /locus_tag="L2X49_004915"
     CDS             complement(1639..2280)
                     /locus_tag="L2X49_004915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799851.1"
                     /translation="MRIKHLIFLPVPFISFYLNAASLDEINQKLDEGYRYACNATQTG
                     NAEAASLALENYRTKFINSASRSGFSEIMNVEINKVVAEFFRKGMIQGNTSTGACETP
                     AELLLQTSQAVYSRQSKSALASALDGVRFTEYAAQAYPIFYSIQSTGISEDEVIEQIN
                     KIGNNAIINNQNAIDVSNATNAMKEVAKVLFSARSDPELMKFMMDYINNHKLH"
BASE COUNT          593 a          572 c          518 g          690 t
ORIGIN      
        1 cctaccgcgc aaaaatccga aactcctgat ctagttatta gaccttacgc cttaattcta
       61 atgtatgcgt ccattagcat taagcgaaga cggacttatt caggatgttt ttaccaaaga
      121 agaaccactg ggattggttt tgatggctgt gccaatttat attaccggtc tgatcatgat
      181 aggtactctg ttcacatgta agaaatgaac agcctgtttc tgtttaaggc gaacaggttt
      241 attctcttca cgcttccatg cccccccata atttcccatc cctcttcaga gcaatgagta
      301 attttttggt ccttcagatt ctcccaccct attgtcttcg caggccgtca ggcagacact
      361 tttaagccgg tggttataga gaccaatacg gacgatttcc tggagataat ccctttcctc
      421 caccgttagt aactgccctt cctttgattt tgtaatcatg tcctgccagt taagcaattt
      481 ctcgtaattc acatgctcac tggtgtcgag gcaatgcagc cggaagtaac gcataaacag
      541 cgggaattcg tcggtctggg cgatatctga agctctgacg gtgagccgac acaaacacac
      601 aatggtggcc tgctgcattc ttatcagcac attcatgctc tggggtaagt tgtccggcag
      661 cttcagggtg ctgtcggaaa ggcgttgtag aagtggtcct gcttccgctg gctcgcggcc
      721 ctcatatacc ttcaggggct cggtccccag ttcctgcatc atggggtgat acaaccattc
      781 gcgtgactcc gtcgcatcaa tgatgatttt ctcgatacat tcactgtagg taccatctcg
      841 tctaccaagc tgaacctgac tgatttcgta ttctatatcc ggcagatcaa tacgcaatgc
      901 agctaccatc tccgaggcat ggagatggtc gaacgtcagt tttccggcca cactgcggtc
      961 aatgtccaga aacacagcta tgcagagatc gttcaccttc acaaacagat ctgccgttgc
     1021 gccgaacatg cttccgctgc cgctgacttc atcaagtggg accgtgcctc catcgtaacc
     1081 ggccagaaga tgcgggaatc ctagaagttg tttttcctga ataacttcaa taatttttcc
     1141 ccgaatcgcc tccatcaggc catcacaatc accgaggaga tcggtactgt ggctatagtg
     1201 ccagagtttg acctctccct gtctggctat caaatccgta cgacacccag gacagatgca
     1261 gttgcatgcg cttcctctgc tgacttccgt gatgtccaga aaccgtccgg tatcgcgatg
     1321 gagtccgaaa ggaatcccat aggtatagct ttttcgcatg tcagtggccc cgctgaatac
     1381 ataccttcac tatatcgggc gaagacagca tgacacttcg ataagaagac tatttttcgt
     1441 gagattttaa taccgtaact gagcggattg ataaaacgat gatgattaga cacgggtgaa
     1501 aagttacttt tccgattgcc aaaacctcca ccagcagtgt gccatattaa tctggacgga
     1561 ttcggaatta acgagcgttt cttcagctga ctcatttttt ggccgaaaat ggctttgttt
     1621 tatacaccgc aattaccttc aatgcagttt gtggttatta atataatcca tcataaattt
     1681 catgagctcc gggtcacttc gggctgaaaa aaggactttc gctacttctt tcattgcatt
     1741 tgtcgcatta ctgacatcaa ttgcattttg gttattaatt attgcattgt ttcctatttt
     1801 attaatctgt tcaattactt catcctcact gatacctgta ctttggattg aataaaaaat
     1861 cgggtaggcc tgtgccgcat attcagtgaa tcggacacca tcgagggctg acgccagtgc
     1921 tgactttgac tggcgggaat aaaccgcctg agaggtctgg agcagcagct ccgctggagt
     1981 ctcacaggcc ccagtgctgg tgttcccctg gatcatgccc tttctgaaaa attccgcgac
     2041 aactttgttt atttctacat tcattatctc gctgaaacca gatcgtgatg ctgagttgat
     2101 gaattttgtt ctgtagttct ccagcgcgag ggaggcggct tctgcattac ccgtctgagt
     2161 tgcgttacag gcgtaacggt aaccttcatc cagcttttga ttgatttcgt ccaggctcgc
     2221 cgcgtttaaa taaaaggaaa tgaagggaac aggtaaaaat attagatgtt taattctcat
     2281 acgagttacc accatggagc ggaaacagga ttttaaatga cgtgatgact ttactaatta
     2341 agcttatttg aagaggtatt aatagaatat taa
//
LOCUS       DAFNBJ010000306         2365 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.312, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000306 DAFNBJ010000000
VERSION     DAFNBJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.312"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            23..127
                     /locus_tag="L2X49_004916"
     CDS             23..127
                     /locus_tag="L2X49_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001453572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HBN4799852.1"
                     /translation="MNVLLQGQKLTLLDPEMWFLRGNENKDVTIFLIN"
     gene            109..657
                     /locus_tag="L2X49_004917"
                     /pseudo
     CDS             109..657
                     /locus_tag="L2X49_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            689..1981
                     /gene="sctN"
                     /locus_tag="L2X49_004918"
                     /pseudo
     CDS             689..1981
                     /gene="sctN"
                     /locus_tag="L2X49_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
     gene            1971..>2365
                     /locus_tag="L2X49_004919"
     CDS             1971..>2365
                     /locus_tag="L2X49_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HBN4799853.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLKSLLDKKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYVRLKTRIKKTFKS"
BASE COUNT          752 a          423 c          561 g          629 t
ORIGIN      
        1 tgaagaaggg ctgttacttc caatgaatgt tttgcttcaa ggacagaagt taaccctact
       61 ggaccctgaa atgtggttct tacggggaaa tgaaaacaag gatgtaacta tattcttgat
      121 caattagaga tgaagtatcc tgatctatta aaagagatat taagatatat aaccgtgcag
      181 cgcatttcag aggtaattca gcgtctgatc ctggagcgaa tttcagtgcg caatatgcgg
      241 ttagttatgg aggccttagc attatggagc ccacgagaga aagatataat cacactcgtc
      301 gagcatgtac gaggtgcatt aggtcgctac atttgccata agttttcata ttctggagaa
      361 attaaagcaa ttgtgatttc gcctgaaata gaggacagaa tcagagatgg tgtcaggcct
      421 accgctggcg gtacttttct taatctcgat gcatccgaag ctgagatgat tctggataat
      481 tttaaactgg cactttctgg aatcaatatt cctattaaag acattatttt actgggatct
      541 gtggatattc gtcgttttat caaaaaactt attgaatcta gttatcgaga tctcgaagta
      601 ctttcgtacg gggaactaac ggaaaatgtt cccgttaata ttctgaaaac tatttaggat
      661 aatgtcttgc gatatggagc atgccggtat gaaaagatta aaattattaa ataaatacag
      721 ctacttacat agcataaacg gctctctgat tgaagcagaa cttgacgacg tttcagtggg
      781 tgaagtatgc gaaatttatg caagctggca agcaaatgaa cgtatagcca gggctcaggt
      841 cgttggcttt cgcaatggaa agacattatt aaaccttata ggcagctcag tggggcttac
      901 acgtactgca gttttgaaac caacgggaga gcagttaacc atacagatca gtgatgcctt
      961 tctttgttcg gttttgaatg cctctggtca aattatggaa aggtttgtcc cgaaccctcc
     1021 aggcgataga ggaaatttac gcctgattga tgaactgcca ccgtcgtatc aggaacgtcg
     1081 agttattaat acaccactag agacacgcat aagagtcatt gatggcgtac tgacatgtgg
     1141 cattggtcaa cgtgttggga tttttgcatc ggctggctgt ggcaaaacgg ttttaatgca
     1201 tatgctcgta aacaatactg aagctgatgt gtttgttatt ggcttgattg gcgaacgtgg
     1261 tagggaagat acggaatgcg cggaatcgat gaaacaatca gtaaatgcgg caaaatgcgt
     1321 attggtttat gccacctctg atttctcgtc agtagatcgg tgtaatgccg ccctgatggc
     1381 gacaaccgtg gcagagtatt ttagagatcg aggaaagaga gttgtacttt ttatcgactc
     1441 aatgacgcgg tatgcatgag cactacgtga tatgaaactg gcagcgggtg aaccacctgc
     1501 cagaggtggt tatcctgcat ctgtttttga cagtttgcct cgcttactgg agaggccggg
     1561 accaacacta aaaggaagta tcaccgcgtt ttataccgtg ttactggaag gggaagacga
     1621 atcagacccc ctgggagatg aaattcgctc aatcctcgat ggccatatct atctgagtcg
     1681 caaactcgct gggcaaggtc actatccggc aataaatgtt ctaaaaagtg tgagtcgagt
     1741 atttggtcag gtcactgatg aaaaacacag agataatgct gcacgggtaa ggaaaatcct
     1801 cacaactttg gaagatctac aggtttttat tgacttaggg gaatatcgcg ctgggcaaaa
     1861 tgctgaaaat gatttcgcta tgaatgcacg gcctaaactc acaaattggt tgaaacagtc
     1921 agttaatgaa aaaatgccaa tgagtgaaac tctaaaagag cttgaacgaa ttgttaagta
     1981 aagttaatcg acttatcaga agaacagcgc agagcctggc tgcatgtgaa gcatcattac
     2041 aaaagttaaa tgcagaaaaa gaaaagttag ccgaaaagga acgcctgtat gatatgcaac
     2101 ttaaaaacct gaagtcactg ttagataaga aggagttact cggcgaagtt gtatttcgac
     2161 aggatatatt ttactcgtta cgtaaagttg cagtcattca gcagcagatc gctgagataa
     2221 atcttgaaaa gcaaaaaata gcagagcgga gaaaaatact taacaaggaa atagttcaac
     2281 agcaagcaca aagaaaacac tggtggctaa aaggagaaaa gtacgttcga ctaaaaacgc
     2341 gcatcaaaaa aacttttaaa tcaga
//
LOCUS       DAFNBJ010000307         2359 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.313, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000307 DAFNBJ010000000
VERSION     DAFNBJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.313"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(58..720)
                     /locus_tag="L2X49_004920"
     CDS             complement(58..720)
                     /locus_tag="L2X49_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBN4799854.1"
                     /translation="MTQTVPKGFVRAYLRASTTEQDATRALDTINTFAIERGLSICNY
                     YIENESGSKLERPELFRLLKDCQQNDILLVEDVDRLSRLVGEDWNTLKKMIRQKDIRV
                     VAVNVPTTWLASGHNDFDSRMFSAINDMLLDMLAAVARRDYEQRRERQQQGIARAKRE
                     GKYKGRQVNQSRYDAINRLIASGSSWSQIQKVLGCSRATISKAVKQQTVNKMNHEPEG
                     RE"
     gene            1101..1763
                     /locus_tag="L2X49_004921"
     CDS             1101..1763
                     /locus_tag="L2X49_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-arginine deiminase"
                     /protein_id="HBN4799855.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHD
                     LLSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVS
                     DIVVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDL
                     DKLLRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIG
                     IL"
     gene            1863..2147
                     /locus_tag="L2X49_004922"
     CDS             1863..2147
                     /locus_tag="L2X49_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBN4799856.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVAGKPKLMNFR
                     LPAYFEEILEAEAQRTGQSKTTVLKAALAAFDNLDENLKNHWLLESAKLG"
BASE COUNT          713 a          553 c          522 g          571 t
ORIGIN      
        1 catatacagt ataaagaatc agttttattc ttcaacacac tgtttattat gttgtaacta
       61 ctccctacct tcaggttcat ggttcatttt gttaacggtc tgttgcttca cagctttgct
      121 gatagtcgcc cggctacaac ccagcacttt ttgaatctgg ctccaggagc tgccgctcgc
      181 aatcagcctg tttatggcgt catagcgaga ctgattaacc tggcggcctt tatacttccc
      241 ttcccttttc gccctggcga tcccctgctg ctgccgttcc cggcgctgtt catagtctcg
      301 cctggcgacg gcggccagca tatccagcaa catatcgttt atggccgaaa acattcggct
      361 gtcaaaatca ttgtgacccg atgcgagcca ggttgtcgga acgttgacgg ccacaacccg
      421 gatatctttc tgccggatca ttttcttcag tgtgttccag tcctccccta ccaggcgtga
      481 aagcctgtcc acatcctcta ccagcaagat atcgttttgc tgacaatctt tcaggagccg
      541 gaacaactca gggcgttcaa gcttagaacc tgattcattt tcaatgtagt agttacagat
      601 gctcaggccg cgctcgatgg cgaaggtatt tatcgtgtcc agggcgcgtg ttgcatcctg
      661 ttcagtcgtg gatgcgcgta agtaagctct gacaaagcct ttcggtactg tttgagtcat
      721 tatagggaac cagttcattt tgagttaacc agacacaccc atacagtacc catttcagtt
      781 ggttcaggga aggcacactc aaaaagaacc agcaaacaga ccccagccct atagtgacaa
      841 cgtcacacta aaaatttaca acataaagat gatgtaaaga cacaccaaga tttacaccat
      901 aaagcgcaca taaagacaca gcaacattta ccatgtaaag acaccataaa gataaatata
      961 tctttatttg tgctttacat ggtaaagcca ttgcattata aagacactgt aaagacaaaa
     1021 gtatctttac gaaaaacaca ccatgtaaag acattcatat ctttacatga gctttacaac
     1081 ataaagagag ggattctgaa atgggtaaaa tcttgcttgt cgtatcagat aaaggtgggg
     1141 taggcaaaag cacctacgtg gctaacacag gctcaatgct ggtcaacaaa ggtaagtcgg
     1201 taattatcct gaagacagat aaaaaccacg atctgttgag ctggaacgaa aagcggacag
     1261 ataacggctt actcactatc ccggttcacg cagcttacgg aaacgtcagc aacgaaatta
     1321 agcgccttag taagctgtgt gaagttctca ttgtcgattg ccccggccac gacagccagg
     1381 agttccgtag cgccctgaca gtatcagata tcgtggtgac actggttaag ccgtcctctg
     1441 atttcgaaag cgaaacactg accagtgtta cagagaaaat ccgcaccgct cagaaagcta
     1501 acgcagctct ggaaccgtgg gtgctgttca ccagaatcaa ctcagcgaaa ccccgccaca
     1561 gaaaagcggc cattgatctg gataaattac tgcgttcgga caacatctgg attcaacctc
     1621 tgaaaacccg gatatccgag ctggatgtgt atgaaagcgc ctgtaatgaa ggggcagggg
     1681 tacatgacgt tagccgggca tcgagccttt caacagcgaa agcacagatt gaacttgtgg
     1741 cacaggaaat tggcatttta tagaaatatc aacaatattc atcgaaatct ttacattgta
     1801 aatacaccat aaagatacta tgatatttac aattaatgca taccgtacaa ggggaaattt
     1861 tcatggcttt aaaactaaac cgcccgaaca tcgatgaatc tcagcaacca gcaacaaacg
     1921 cagaaaccgc gcgttttatc tccggcgcta ccagagcgcc tgtagcgggt aaaccaaagt
     1981 tgatgaactt cagactgcca gcgtactttg aggagatcct ggaagcggag gcgcagagaa
     2041 caggccagag caagacaacc gttctaaagg cagcgctggc cgcattcgat aacctggacg
     2101 agaacctgaa aaaccactgg ctgcttgaat ctgcaaaatt agggtagcgt tatggctaag
     2161 aaaaagagat atgttgtgat ggttagagat aaaccaaata cagcggcaat cagcgattat
     2221 tagcctggtt agtatggttc gctaacaggc ataataaggc cgttgcctat gactgcggag
     2281 aatggattgt cgaaccatca cattggttaa gaattggaaa tccaaaatag aaagaaacct
     2341 aaggaaagaa aaatgttca
//
LOCUS       DAFNBJ010000308         2334 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.314, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000308 DAFNBJ010000000
VERSION     DAFNBJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.314"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            121..624
                     /locus_tag="L2X49_004923"
     CDS             121..624
                     /locus_tag="L2X49_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN4799857.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(670..1086)
                     /gene="rraB"
                     /locus_tag="L2X49_004924"
     CDS             complement(670..1086)
                     /gene="rraB"
                     /locus_tag="L2X49_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBN4799858.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1248..2252
                     /gene="argF"
                     /locus_tag="L2X49_004925"
     CDS             1248..2252
                     /gene="argF"
                     /locus_tag="L2X49_004925"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBN4799859.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNNKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          594 a          548 c          588 g          604 t
ORIGIN      
        1 caatactctt atcagtaata ggctggcaaa aactttattg ctttataaga cgcttgcgta
       61 cgtaagaaga gagtggtatt aaacaaatgt ataattactt tatttttatt gggttaaact
      121 atgaataaca ttgcgccgca atcacctgta atgcgtcgcc tgacgttgca ggataatcct
      181 gctatcgccc gcgtcattcg tcaggtatcc gccgaatacg gtcttaccgc tgataaaggc
      241 tacaccgtcg ccgatccgaa tcttgacgag ctgtatcaag tgtatagtca gcctggtcat
      301 gcatattggg tggttgagta cgagggtgaa gtggtcggcg gcggtgggat tgcgccatta
      361 gcaggtagtg aatcggacat ttgcgaactg caaaagatgt attttcttcc ggctattcgc
      421 ggtaaagggc tggcgaaaaa actggcctta atggcgatgg agcaagcacg agagatgggc
      481 ttcaaacgct gctatctgga aacgaccgct tttttaaagg aagccattgc gctttatgag
      541 catttgggct ttgagcatat cgactatgcg cttggctgca cgggccatgt cgattgtgaa
      601 gtgcggatgc tacgcgaact ctaaaaaaat gcccgctagt gtgtgctatc gggcatttta
      661 atcaattaat tagtggcgaa ccccgtcatc gtcttcatcg acaaaatctt catcgtcgcc
      721 gtcttcgccg ttgggatctt caaagtaagt gccccaaccg tcgtactcaa cgtcaaattt
      781 ctccgccagc gtcatcagtt gttcaacctg ggcatcgatc agatcggcat tcaacgcgca
      841 ctcgctgagg atgtcgcagc aaatcacgat atcaccatct tcaacttcca gctcttctgg
      901 atcggtcact tcgtaaccga gtttaaacgc ttcaactgct gctttttcca gggtttccag
      961 atcgtctgcg gaaagatggt gttcgatggt gtacagcgcg tccggatcgc tgccatcttc
     1021 cagtaattct tcaataatca aacgtgtttc ttcacgctgt tcttccagtt gttccgggtt
     1081 tgccatggct cattcctctt aatgtcctgc cgatactctt attgtcacac accactgaca
     1141 ttgcctccac ctttgtgaca aagatttatg ctttagactt gcaaatgaat aatcatccat
     1201 ataaattgaa ttttaattca ttgaggcgtt agccacagga gggatctatg tccgggtttt
     1261 atcataagca tttcctgaaa ttactcgatt tcacgccagc tgaactcaac agcctgttgc
     1321 agttagccgc gaagctgaaa gccgataaga aaagcggtaa agaagaagcc aaactcactg
     1381 gtaaaaacat cgcgctcatc ttcgaaaaag actcgactcg tacccgatgc tctttcgaag
     1441 ttgccgcata tgaccagggt gctcgtgtta cttatctcgg cccaagcggc agccagattg
     1501 gtcataaaga gtcgattaaa gacactgccc gcgtgcttgg tcgcatgtat gacggtattc
     1561 agtatcgcgg ctatggtcag gagattgtcg aaacactggc ggaatacgct ggcgtgccgg
     1621 tatggaacgg cctgaccaat gagtttcacc ccacgcagct gctggcggat cttctcacca
     1681 tgcaggagca tttgcccggc aaagcattca acgaaatgac gctggtctat gcaggtgacg
     1741 cgcgtaacaa catgggaaac tcgatgctcg aagctgcggc gcttaccggt ctggatttgc
     1801 gtctggtcgc gccacaggca tgctggccgg aagctgcgct ggttacggaa tgccgcgccc
     1861 tggcacagca aaatggcggg aatattacgc tgacggaaga tgtagccaaa ggagtagaag
     1921 gtgctgactt tatctatacc gatgtatggg tgtcgatggg ggaagcaaaa gagaaatggg
     1981 cggaacggat tgcattgctg cgtgattatc aggtgaataa caagatgatg cagttgaccg
     2041 gtaacccgga ggtcaaattc ctccactgcc tgcccgcgtt tcatgacgac cagacgacgc
     2101 ttggtaagaa aatggcggaa gagtttggcc tacatggcgg aatggaagtg actgatgagg
     2161 tcttcgaatc tgccgccagc attgtttttg atcaggcgga aaaccgcatg cataccatca
     2221 aagcggtgat ggtcgcgacg ctcagtaaat aattgatatc gctataaaga tgccgggaaa
     2281 tacaaaaaca tcccggcatc acattatttt atgcagtgat aggtctattc ttcc
//
LOCUS       DAFNBJ010000309         2322 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.317, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000309 DAFNBJ010000000
VERSION     DAFNBJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.317"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            376..1629
                     /locus_tag="L2X49_004926"
     CDS             376..1629
                     /locus_tag="L2X49_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBN4799860.1"
                     /translation="MNLIDKIALVGQRMKSEQISLKESLLVSSRVSVSDDSVDGVDRL
                     IYNHCLNKKNLSDFFGKSRVTFNKILADLEEKELVGAPIYQNKNHLYTRWDVQKIMDA
                     LNYPRYSDYYCSRTIVTQNHKGGTGKSTTSGVLAVAAALDLHLNARILLIEWDPQGSI
                     GSGMIQSVAEDDVFLTAIDAILGVYEEDSDYRKYLDLGYSEEQIIEGMPFSTHLPNLD
                     VITAFPTDARFKDKYWQCSREERTELLLRFKEVILPVLKSKYDLIIIDTPPEDSPITW
                     AADEAADGILVAVSPREYDYASTTDFMLTISERFKQSPSKGENLSWFKVLAVNVDDKS
                     PYEKIVLDKLVRTVQELFMSANIKNSEAFKAAASRGRTVLDIKKSEELCSPKQLDVAE
                     ESVMAVYQQFINEIKSFSVKQGGNV"
     gene            1626..>2322
                     /locus_tag="L2X49_004927"
     CDS             1626..>2322
                     /locus_tag="L2X49_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HBN4799861.1"
                     /translation="MSDEQHIGNDKSRYINAPKRTEVIHRSGLQGLKGQPRLKKLFTL
                     HNGRKLEAEHIIVPAEKVELETTVHPLNPRNQEALSVNAVRDILKDIEARGVDTEGVA
                     VKRNGVYLLIEGSRRRFCCIQSAKELPLWVLPDDVNADDINSIISATQTSRRFSYREV
                     GLKYLREMEEHGFVTNEELANYHGISHVSVSKRVQAAKINSTLIALFPDYEAIPNSYY
                     NRLFRLQKYIEKNL"
BASE COUNT          708 a          451 c          535 g          628 t
ORIGIN      
        1 cttaatcgcc aggccatgga ggaatgctgg tctcgtacca tggcatttaa cccccttaat
       61 cgccagacta tggggtaatg ctggtcgggt accgtgggat ttacccccct tattgccaca
      121 tctatggagt gatcctgcta tgtcggaaga ttggtattgt tgatacttaa gaggcttttg
      181 gaagacttca atgaactgcc aattgaaatt caataatgca gatattccaa aatctaagaa
      241 aagaatacag aacgttagcg taataataga attaaggtta ctattaaacg cataacgcta
      301 aagtatacgc attatgcgta aagtttacgt atatacgttt aatttcacta tttaggtaaa
      361 agggatcgga agagaatgaa tctaattgat aagatcgccc ttgtcggtca acgcatgaag
      421 agcgagcaga tctctctgaa agagtcatta ttggtctctt cgagggtttc ggtatcggat
      481 gatagtgtag acggtgttga tcgcctaatc tataaccact gcttgaataa aaaaaatctg
      541 tcagattttt ttggtaaatc tcgcgtaact tttaacaaaa tcttagcgga tcttgaagaa
      601 aaagaactgg ttggtgctcc tatctaccag aacaaaaacc atctctatac ccgctgggat
      661 gtccagaaaa taatggatgc tctgaactat cctcgatata gcgactacta ctgtagccgt
      721 acaatcgtaa cgcagaacca taagggtggt acggggaaga gtacaacttc aggcgttctt
      781 gcagttgcgg cagcacttga tttgcatcta aacgcgcgga tactgttgat cgaatgggac
      841 ccacaagggt ctattggaag cgggatgatc cagagcgtag cggaagatga tgttttcctg
      901 actgctattg acgccatctt aggggtttac gaagaagact cagattacag aaaatatctt
      961 gatttagggt actcggaaga acaaattatt gaaggtatgc ctttttcaac acaccttccg
     1021 aatcttgatg ttattaccgc attccctaca gatgcacgtt tcaaagataa atactggcag
     1081 tgttccagag aagaacgaac tgagttatta ctacgtttta aagaggtaat cctgccggtc
     1141 ttgaagagca agtacgacct gatcattatc gatactccac cagaagattc gccaatcaca
     1201 tgggcagcgg atgaagctgc tgatggcatt ttggttgccg tttcacctcg tgagtatgat
     1261 tatgcctcaa caactgattt catgttgacg atcagtgagc gattcaaaca atcgccgagc
     1321 aagggcgaaa atctaagctg gttcaaagtg ttggcagtga atgtcgacga taagagtccc
     1381 tatgaaaaga ttgtcctgga taaactggta aggacagtgc aggaactctt tatgtctgca
     1441 aatatcaaaa actcagaagc gtttaaagcc gcagcttcta ggggtagaac cgtattggat
     1501 atcaaaaaat cagaagagct ctgctctccc aaacagcttg atgttgccga agaatccgtg
     1561 atggcggtct accagcaatt tattaatgag attaagagtt tttcagtgaa gcaagggggt
     1621 aatgtatgag tgatgagcag catatcggga acgacaaatc ccgttatatt aatgcgccta
     1681 agcgtactga agtaatccat cgctccgggc ttcaaggcct gaaaggtcag ccacgcctga
     1741 agaaactttt tacattacac aatgggcgga agcttgaggc ggaacacatc attgttccgg
     1801 cggaaaaggt ggaactggaa accacagtac accctctgaa cccacgaaat caggaggctc
     1861 tttctgtaaa tgcagtaaga gatattctga aggacattga agcaagaggt gttgatactg
     1921 agggcgttgc agttaagcgg aacggagtgt atctgctaat agaaggtagc cgcaggcgtt
     1981 tctgttgcat tcaatcagca aaagaattgc cattatgggt tttgcctgat gatgttaacg
     2041 cagatgatat taattccatc atatcagcaa cacaaacttc tcgacgcttt tcatatcgtg
     2101 aagtaggttt aaagtattta agggaaatgg aagagcatgg atttgttacc aatgaagagc
     2161 ttgcaaacta tcatggtatc agtcacgtgt ccgtctctaa gcgagttcag gctgcaaaga
     2221 ttaacagcac tcttattgca ctgtttcctg actatgaagc tattccgaat tcttattaca
     2281 atcgcctatt ccgacttcaa aaatatatag agaagaattt at
//
LOCUS       DAFNBJ010000310         2318 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.318, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000310 DAFNBJ010000000
VERSION     DAFNBJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.318"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..431)
                     /gene="sctJ"
                     /locus_tag="L2X49_004928"
     CDS             complement(<1..431)
                     /gene="sctJ"
                     /locus_tag="L2X49_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HBN4799862.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALP"
     gene            complement(472..687)
                     /gene="sctI"
                     /locus_tag="L2X49_004929"
     CDS             complement(472..687)
                     /gene="sctI"
                     /locus_tag="L2X49_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HBN4799863.1"
                     /translation="MIDLNDRVLNLDNPDDKMISAFANYAVQTENWQQNALQALRSDK
                     EGLTPEKLLVLQDHVLNYNVEVSLVEH"
     gene            complement(779..1019)
                     /locus_tag="L2X49_004930"
                     /pseudo
     CDS             complement(779..1019)
                     /locus_tag="L2X49_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(1033..2214)
                     /locus_tag="L2X49_004931"
     CDS             complement(1033..2214)
                     /locus_tag="L2X49_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HBN4799864.1"
                     /translation="MENNDKFLSQELLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIFI
                     AFYIVNASNDPQLRHIDKILVNKNRNYEILYGRDHVIYINTNILDEAVWVKQALEKNQ
                     PGKPVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDASNLRLTVSKQRNAITQQLID
                     NLIKGLLQTMPYASNISIAVLDDNVLESQAIETLSAIGLSYEKYKTANNVYFNIIGTL
                     SDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNWTFQ
                     E"
BASE COUNT          719 a          452 c          364 g          783 t
ORIGIN      
        1 attggtaaag cagttttacc actatcgcca ttatcaacat cgtaactaac atgtactctc
       61 gaagaaataa tgccatccat aattttgaga gattgctcta accgctgctc aatagcagaa
      121 tatagccttg ctttttccgc tcgtggagat gaaacgagtg catctgcagg gaacatctgc
      181 gatatttgaa tatcaggctt acccggtaga ttgtagattt ttagccaatc caccgcagaa
      241 gcaaaatccg ttggttcgac aaatattgaa aatcctgttt tgccttgatc cttcttttca
      301 gcattaatat tatgtctttg taaaacagca aggacatcat tagcttgctg ttgatcaaga
      361 tggttcaata attcctgctg cttgcagccg cacaacagca ggataaacaa taataatgat
      421 atatatttca tgagcgggtc aacgtctcaa ctgcagcgac aatctttctt gctaatgttc
      481 caccaatgaa acctcgacat tataattaag cacatgatcc tgcaagacta ataatttttc
      541 aggagttaac ccctctttat cagaccttaa tgcttgtaac gcattttgct gccaattttc
      601 agtttgtaca gcatagttgg caaaagcgga gatcatttta tcatccgggt tgtcaagatt
      661 gagaacccgg tcatttaaat caatcacttt cgcttgctgt atttcatgag cagttgatta
      721 ctcagctcta tcaataggtg gcatattgct tactgacata ataaaatcct tatccagttt
      781 agcggaagtt ttgtatgatt gctgaatcaa gatctttata cgcttttaca acgttagatt
      841 gcgcatttcg atataatgta tactcagcta atgcactttg atattcggca agaaatttcg
      901 tgtcggaggg attggttggc taaatcctcc aacgcttttt caacttgctg gttcagatca
      961 tcaacgccct gatcaaattg attgctgata tccataatat aaccattcca atctgccata
     1021 agtcctcctc aattattcct gaaatgtcca gttgctacct tgagacttct cgaatcggtt
     1081 atctccgtag ctaaatgaac tattatttgt gtcctgattt ttcaaattca cattaaatct
     1141 tacatattgt ttaccccatt gtttataata ttcgtcaaca tagttattaa ttttatttaa
     1201 ttcactgtca cttaacgtac caatgatatt gaaatacaca ttgttagctg ttttatattt
     1261 ttcataagaa agacctatcg ctgacaacgt ttcaatagcc tgactttcca atacattatc
     1321 atctaatacc gcaatactta tattgctggc atatggcata gtttgcagta atcctttaat
     1381 aagattatcg attagttgct gtgtgatagc gttccgttgc ttactcactg tcaatcttag
     1441 attactggca tctaatagtt gaagtttaaa atattgtaaa tcagggaaat tatcagcaag
     1501 ccatgaaaaa attcgtatcg attcatcatc aggatttatc acccgtactg gctttcctgg
     1561 ttgatttttt tccagtgctt gtttgaccca gactgcttca tccaaaatat tggtattaat
     1621 atagataaca tgatctctac catataaaat ttcataattc ctgtttttgt ttacaagaat
     1681 tttatcgata tgtctcagct gcggatcatt actggcatta acaatataaa aagcaatgaa
     1741 aatgatagca aaaagaaaaa aaagcattac atacttaaac agcttactgc taaagaaatg
     1801 ttgttttgta gaaataatgt tattttcaat aatgcctggc aatgaatact gccaaacttc
     1861 atttttttct tttacagcaa atttgagacc taaaacttct atttgctgat tcaatgttaa
     1921 cgttctatct tcaggttctt gtgctgttaa ttctctgaat gttactacca gatcagggtc
     1981 ggtagtaatt attatctcaa aactacctgt aagctcgcca taaggaacga ctattgtgtt
     2041 ttctggtaac tcagaaaaag catcacttct tcctaacgaa acatcattac caatgataac
     2101 aagtagacgc ccattaagta tttcatactc gcatccattc aatggtccgc tcaacaaacg
     2161 aatggcataa gattccaata attcttgtga taagaattta tcattatttt ccatgaatac
     2221 cacccgctaa tttcataagc tctagtttag agtagtgtaa caagacggaa atatcatgac
     2281 caaattatga tgacacagaa taaaaaaatt ataatttc
//
LOCUS       DAFNBJ010000311         2291 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.319, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000311 DAFNBJ010000000
VERSION     DAFNBJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.319"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..162)
                     /locus_tag="L2X49_004932"
     CDS             complement(<1..162)
                     /locus_tag="L2X49_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799865.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAP"
     gene            complement(309..>2291)
                     /gene="pdeR"
                     /locus_tag="L2X49_004933"
     CDS             complement(309..>2291)
                     /gene="pdeR"
                     /locus_tag="L2X49_004933"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBN4799866.1"
                     /translation="KTVRESTTLYTFLGSHNPYWRLTESSDVLRFSTTETTEPDRTLQ
                     LSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAVA
                     RDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLFM
                     SRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLICS
                     GTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDNF
                     KKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMAS
                     RILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQFC
                     VFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPERG
                     LIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQTI
                     FTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGYS
                     SLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKED
                     AFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT          615 a          560 c          531 g          585 t
ORIGIN      
        1 cggtgccttc gggatgtctg gcgtagattc agcaacctgg cgcaagagtt ctgctttaag
       61 tatctcgaga ttatggatcg cagagtggta atcgcctgcc acaaggatat ctaacacaat
      121 atcaatacgt tcggcaacgc gctgggcatc aaattcagac atcaaggaca tccttatagc
      181 aaaagtggaa aatgaataat gcaaaaattg ccgctaaaag agaagtgttt aacagcaacg
      241 gctaattatc atccaggaat acgatatata atgacgggat atagcgctaa gtatatatat
      301 tcatctactt atgcgcgctt cagatagcgt ttataccagc gttcgaaggc gacggcgggc
      361 atcggtttgg caaacaaaaa tccttgccgc tcattgatcc cgttcttggt taaaaaagca
      421 tcttcctttg cactctctac accttcggcg atcacctgaa gattcaatgc ctgggccaca
      481 gcgacgatcg cccggaccag tgactgcgag acaggttgtt tgtgaatatc tcgaacaaaa
      541 acctggtcaa gtttgatggc atcgatcgga aagcgcgcca gttgcgaaag tgaagagtag
      601 ccggtaccaa aatcatccag atgcacttgc gcacctagtt ggctaaattg ttgaataaca
      661 gacagtgcca gttcatcatt ctcaatcaga caactctctg tcagttcaac atctataggg
      721 cagtattcaa aattgagttc ctggagaacc tgtttcaggg cggtgaaaat ggtttgatcg
      781 gcgagctgac gtgcagaaat atttaccgcc actcgcaggt ttatgccttt atcccgccac
      841 tttgccactt ggcgtacgac atcgagaatc acccaacggc ctaaaggcac aattagccct
      901 gactcttcgg cgtaggaaat gaagtccaac ggtggaatca acccacgttc aggtgactgc
      961 caacgtacta gtgcttccag actgcgcact tcgccacgcc aggtgatttt cggttgatag
     1021 tgaataacca actgatcgtt ttccagtgct ttacgcaagt tggtatccag ccagagatat
     1081 tcaaataccc gttgattcat ttctggggta aaaacgcaaa attgtcctcg tccgccttcc
     1141 ttcgctgtgt acattgctgt gtcggcgtga cgaataatag ccgtgctgtc tgaaccatgt
     1201 tcgggagaga gtgcaatacc tactgaacag ctggtataaa cttcaattaa accaatgcga
     1261 aaggggagcc gtaagcgggt caaaattcgt gatgccattg cttccagcgc gctttgtgag
     1321 gtgttggatg ccagtaccag aaactcatcc ccacctggac gcgccaacac ctggtcatgt
     1381 tcgagacagc ttaaaatagc caatgacacg tcgcgtaata actggtcacc aaacaaatgc
     1441 ccataggcgt cgttgacctt tttgaaatta tccaaatcaa gatacacaac cccaacttta
     1501 ttgttatctg catgattaat agcgtgatcg attaaatcct gcattgcgtt acgattcggc
     1561 agtccggtga tactgtcggt atttgccaga atacgcagtc gctcctgagc gcggcgctct
     1621 tcggtaatgt cggtgccgga acagattaaa aaaatctcgt ttttgccact gccgctgtgg
     1681 acaaatttat tgcgaaacag aaacagccgc tggcctttac atgttggtat ccacagttcg
     1741 acttcatatg cattgccgct tcgaaaaaat acacggttat tgcgcctgga tgccgcagct
     1801 tcacgacggc tcataaacag tttaaacacg ctttgcccaa tgacgtcgtg ttctttcaac
     1861 cctgtgtaat cttcacataa ccgattgaag cgttgaatat tcccccggct gtcgagaatg
     1921 actattgcgg aatgtgcttc agaaactacc tgctcagcaa aggaaagccc gtgtgataaa
     1981 tcacgagcaa ccgccggtgt gtcatgccat gcagacgcgt tgccagccca ttcccgttta
     2041 ttgatttttc gtcctaccag atgcacagaa agatcgctct catcgacggt cagactcatc
     2101 atcaggctgg aggtgatgac cgtcatttcc ctgatgcgag cagcctgttc ggcagataac
     2161 tgcaaagtac gatcaggttc tgtggtttcg gtggtagaaa agcgcaaaac atcgctgctt
     2221 tccgtcaacc gccagtatgg attgtgcgat ccgagaaagg tgtacaacgt tgtggactcc
     2281 ctaacggttt t
//
LOCUS       DAFNBJ010000312         2218 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.320, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000312 DAFNBJ010000000
VERSION     DAFNBJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.320"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..366)
                     /locus_tag="L2X49_004934"
     CDS             complement(<1..366)
                     /locus_tag="L2X49_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HBN4799867.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYIFDHNAKPDIWDYFKAMGVGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGL"
     gene            complement(375..1142)
                     /gene="sctT"
                     /locus_tag="L2X49_004935"
     CDS             complement(375..1142)
                     /gene="sctT"
                     /locus_tag="L2X49_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HBN4799868.1"
                     /translation="MEEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALFQHYHYELSTLNEIDIALFAAREIIIGLFIACLLSSPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1144..1404)
                     /locus_tag="L2X49_004936"
     CDS             complement(1144..1404)
                     /locus_tag="L2X49_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HBN4799869.1"
                     /translation="MNDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1414..2079)
                     /locus_tag="L2X49_004937"
     CDS             complement(1414..2079)
                     /locus_tag="L2X49_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HBN4799870.1"
                     /translation="MSNSISLIAILTLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGTEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVIISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(2069..>2218)
                     /locus_tag="L2X49_004938"
     CDS             complement(2069..>2218)
                     /locus_tag="L2X49_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBN4799871.1"
                     /translation="RIISLLPESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSG
                     HNNVK"
BASE COUNT          771 a          451 c          365 g          631 t
ORIGIN      
        1 taagccattt actggattca atgaatcaaa cttaaattta acagcttcag ttgctaattg
       61 cattttactt tgaaaccagg agacaagtat tgttgtgaac atgcaaacca gcaaaaaagg
      121 aatgatcgtt ttcaaccaac caacccccat agctttaaaa tagtcccaaa tatctggttt
      181 agcgttatga tcaaagatgt attcaagaat cgacataatg tgatgcacat caaaaacata
      241 tccgagatat aaagtaccca caagcattat tacagagatg gttaagtctc tactttttag
      301 aatttgccct tttttagaag catcctggag tttcttttgt gttggctttt cagttttatt
      361 ggccatgaaa cactctattt ctcaatgtat agttgcaatt gttctgggaa aaaggataac
      421 ggcattactc gttcagccag tattgggcca aaataaataa ttaatattaa aaatgccagc
      481 gcacttttga cagtcagtga aatggcaaaa gcatttagtt gtgaagcata acgtgccagc
      541 aatcccagga cagcctcacc gcccagcata acggcaatca cggggcttgc gtaaacgata
      601 gtatgagtca ttatgttatt aatatatgag aataaaggct ctaatttagg aaaattaaaa
      661 ttgcctgaag gccataaatt ataactttgc caaagagttt ccagaataaa gttcaatcca
      721 ccgttagtaa gataaaccgc tgcagagaat aggttaaata atcttgctaa ttccgaagta
      781 tcgacaccag tagcaggatc caaagtacta cttaacgttg caccacgttg gttatcgata
      841 aaactaccta tagcaaggaa aatccaaaac ggggatgata ataagcaagc aatgaacagc
      901 cctatgataa tttctctggc agcaaacaaa gcgatatcga tttcatttaa ggtcgataat
      961 tcatagtggt aatgctgaaa taatgcacat gataccacta ttattattgg atgacggact
     1021 actgttggta tattcccact ggcgaaaaat ggtaacaaat agaaagttgg tgccaatcgg
     1081 caaaaaccga gcgctgtagc agccagcaga ctatgcaact gatagagtat cgcctcctcc
     1141 atcctatcct ctcgcgaatg ctaaattcag catttcgata ccaaacgaat ataatttttc
     1201 accataccat ccgctcatta aaaaaaagca aatacttacg cataataatt tgacgccaaa
     1261 tggcaacgtc tgttcttgta actgtgttac cgtttgaaaa agccctacca ataaaccaac
     1321 aaatgtcgca actgcgatag gtcctgcaga cataactaaa atcagataca gcgccctatt
     1381 ccctgcaaaa acaatatcgt tcatattttt tcctcaggga ttaatggata ggtcaaaata
     1441 ttgtaaaatg agtccttttg ataacattgt ccaaccgtcc atagcgacaa atagaattaa
     1501 ttttatcggt gtagatatga ttaccgggct catcatcatc atacctaacg ttaacagaac
     1561 gctggatata accagatcca ctacgacaaa gggtaaatag atataaaaac ctataataaa
     1621 tgcacttttt atctcactca atgcatatgc gggcagtaac gagaaaattg aaatattatc
     1681 atcatcgatt atctcttcat tgtcttcaga actattcacc ttctgaatct tttcaaaaaa
     1741 gctaaccaac tcaggttcag aatattttat caaatatgat ttataaccac tcatgcctgt
     1801 ctcaacaaaa ttaacaaccg acgctacatt attgaatgaa agattctcat tctgactgtt
     1861 ataatatatt tccgtcccaa ccggcatcat tacaaacatt gacaatagca aagcaacccc
     1921 attaagtgtc atatttgatg gcacttgctg aagccccagc gcgtttcgga caataacaaa
     1981 aacaatcgag aatttaataa aatatgtccc ggatgctatt ataaacggca atagtgtaaa
     2041 taatgttagt atagctatca gcgaaatgct atttgacatt attatgccct gataaccaag
     2101 aatgaatctc tacgcccaat ttatcatcca cttcaacaag ttcgccatag ccagttagcg
     2161 cgccatttac acgtatttct atatttttct cagattcagg aagcagagat attattct
//
LOCUS       DAFNBJ010000313         2205 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.321, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000313 DAFNBJ010000000
VERSION     DAFNBJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.321"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>2205
                     /locus_tag="L2X49_004939"
     rRNA            <1..>2205
                     /locus_tag="L2X49_004939"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          570 a          485 c          697 g          453 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacgct
      601 tatgcgttgt tgggtagggg agcgttctgt aagcctgtga aggtgtgctg tgaggcatgc
      661 tggaggtatc agaagtgcga atgctgacat aagtaacgat aaagcgggtg aaaagcccgc
      721 tcgccggaag accaagggtt cctgtccaac gttaatcggg gcagggtgag tcgaccccta
      781 aggcgaggcc gaaaggcgta gtcgatggga aacaggttaa tattcctgta cttggtgtta
      841 ctgcgaaggg gggacggaga aggctatgtt ggccgggcga cggttgtccc ggtttaagcg
      901 tgtaggctgg ttttccaggc aaatccggaa aatcaaggct gaggcgtgat gacgaggcac
      961 tacggtgctg aagcaacaaa tgccctgctt ccaggaaaag cctctaagca tcaggtaaca
     1021 tcaaatcgta ccccaaaccg acacaggtgg tcaggtagag aataccaagg cgcttgagag
     1081 aactcgggtg aaggaactag gcaaaatggt gccgtaactt cgggagaagg cacgctgata
     1141 tgtaggtgaa gtccctcgcg gatggagctg aaatcagtcg aagataccag ctggctgcaa
     1201 ctgtttatta aaaacacagc actgtgcaaa cacgaaagtg gacgtatacg gtgtgacgcc
     1261 tgcccggtgc cggaaggtta attgatgggg tcagcgcaag cgaagctctt gatcgaagcc
     1321 ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
     1381 gttccgacct gcacgaatgg cgtaatgatg gccaggctgt ctccacccga gactcagtga
     1441 aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc
     1501 tttactatag cttgacactg aacattgagc cttgatgtgt aggataggtg ggaggcttag
     1561 aagtgtggac gccagtctgc atggagccga ccttgaaata ccacccttta atgtttgatg
     1621 ttctaacgtt gacccgtaat ccgggttgcg gacagtgtct ggtgggtagt ttgactgggg
     1681 cggtctcctc ctaaagagta acggaggagc acgaaggttg gctaatcctg gtcggacatc
     1741 aggaggttag tgcaatggca taagccagct tgactgcgag cgtgacggcg cgagcaggtg
     1801 cgaaagcagg tcatagtgat ccggtggttc tgaatggaag ggccatcgct caacggataa
     1861 aaggtactcc ggggataaca ggctgatacc gcccaagagt tcatatcgac ggcggtgttt
     1921 ggcacctcga tgtcggctca tcacatcctg gggctgaagt aggtcccaag ggtatggctg
     1981 ttcgccattt aaagtggtac gcgagctggg tttagaacgt cgtgagacag ttcggtccct
     2041 atctgccgtg ggcgctggag aactgagggg ggctgctcct agtacgagag gaccggagtg
     2101 gacgcatcac tggtgttcgg gttgtcatgc caatggcact gcccggtagc taaatgcgga
     2161 agagataagt gctgaaagca tctaagcacg aaacttgccc cgaga
//
LOCUS       DAFNBJ010000314         2180 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.322, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000314 DAFNBJ010000000
VERSION     DAFNBJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.322"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            67..450
                     /locus_tag="L2X49_004940"
     CDS             67..450
                     /locus_tag="L2X49_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799872.1"
                     /translation="MSVLYIQISRNKITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLHDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVMAMLKQK"
     gene            complement(513..956)
                     /locus_tag="L2X49_004941"
     CDS             complement(513..956)
                     /locus_tag="L2X49_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBN4799873.1"
                     /translation="MVISIRRSRHEEGEGLVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGA"
     gene            1113..2042
                     /gene="metA"
                     /locus_tag="L2X49_004942"
     CDS             1113..2042
                     /gene="metA"
                     /locus_tag="L2X49_004942"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBN4799874.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          554 a          564 c          482 g          580 t
ORIGIN      
        1 aaaaaccccg gtccataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt agtcgtaata aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagacgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcat gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgttagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgtattcttc atgaagtggt cgctggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcatgg caatgcttaa gcagaaataa tcgtgtcacc attggtgggt acgaaacctg
      481 aagttcagcc cagcgggatg agaaaaaaac gcctacgccc cgacatatgc cagattcagc
      541 aatggatacg gtttccccaa atcgtccacc tcagagcgtc ccgtaacctt aaaacccacc
      601 ttcttataga acccaaccgc ctgctcattt tgctcattaa cgttggttgt cagttccggt
      661 gccatcgaga gcgcatgctc caccagcatc cgacccacgc cgcagccgcg cacatcagga
      721 tcgataaaca gcgcatccat atgctgccca cttaacaaca taaacccaac cggctgttcc
      781 cgctcattaa ccgcgaccca caacggtgct tccggcagaa aggaacgaac cagctcctcc
      841 agctcggccc gatactctgc tgataaaaaa tcgtgagtgg catcgacaga tcgacaccaa
      901 atcgcaacga gtccctcccc ttcctcatgt cgtgagcggc gaatactaat aaccattttc
      961 tctccttttc gtcattctta tattctaacg tagccttttc cttgaaactt tctcaccttc
     1021 aacatgcagg ctcgacatta gcaaattttc tggttatctt cagctatctg gatgtctaaa
     1081 cgtataagcg tatgtagtga ggtaatcagg ttatgcctat tcgtgtgccg gacgagctac
     1141 ccgccgtcaa tttcttgcgt gaagaaaacg tctttgtgat gacaacttct cgtgcgtctg
     1201 gtcaggaaat tcgtccactt aaggttctga tccttaacct gatgccgaag aagattgaaa
     1261 ctgaaaatca gtttctgcgc ctgctttcaa actcaccttt gcaggtcgat attcagctgt
     1321 tgcgcatcga ttcccgtgaa tcgcgcaaca cgcccgcaga gcatctgaac aacttctact
     1381 gtaactttga agatattcag gaacagaact ttgacggtct gattgtaacc ggtgcgccgc
     1441 tgggcctggt ggagtttaat gatgtcgctt actggccgca gatcaaacag gtgctggagt
     1501 ggtcgaaaga tcacgtcacc tcgacgctgt ttgtctgctg ggcggtacag gccgcgctca
     1561 atatcctcta cggcattcct aagcaaactc gcaccgacaa actctctggc gtttacgagc
     1621 atcatattct ccatcctcat gcgcttctga cgcgtggctt tgatgattca ttcctggcac
     1681 cacattcgcg ctatgctgac tttccggcag cgttgattcg tgattacacc gatctggaaa
     1741 ttctggcaga gacggaagaa ggggatgcat atctgtttgc cagcaaagat aagcgcattg
     1801 cctttgtgac gggccatccc gaatatgatg cgcaaacgct ggcgcaggaa tatttccgcg
     1861 atgtggaagc cggactaggc ccggaagtac cgtataacta tttcccgcac aatgatccgc
     1921 aaaatacacc gcgagcgagc tggcgtagtc acggtaattt gctgtttacc aactggctca
     1981 attattacgt ctatcagatc acgccatacg atctacggca catgaatcca acgctggatt
     2041 aatcttctgt gatagtcgat cgttaagcga ttcagcacct tacctcaggc accttcgggt
     2101 gcctttttta tttccgaaac gcacctcagg aggtgaataa attttattca tattgttatc
     2161 aataagttat caagtatttt
//
LOCUS       DAFNBJ010000315         2173 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.323, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000315 DAFNBJ010000000
VERSION     DAFNBJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.323"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..1519
                     /gene="dcp"
                     /locus_tag="L2X49_004943"
     CDS             <1..1519
                     /gene="dcp"
                     /locus_tag="L2X49_004943"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBN4799875.1"
                     /translation="LAANKSGGLVVNDIAQLAGMSEQEIALAAVAAREKGLDNKWLIP
                     LLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLLGF
                     PHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIHAVIDKQQGGFSAQPWD
                     WAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYHPD
                     VRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQKP
                     AAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHWAT
                     HPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEENE
                     AMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADDGY
                     QWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(1564..2082)
                     /locus_tag="L2X49_004944"
     CDS             complement(1564..2082)
                     /locus_tag="L2X49_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBN4799876.1"
                     /translation="MNINSDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT          532 a          515 c          564 g          562 t
ORIGIN      
        1 acttgcagca aataaatccg gcggtctggt tgtgaacgat atcgcgcagc tggcaggaat
       61 gagtgagcaa gagattgcgc tggcggcagt ggcggctcgc gagaaaggtc tggataacaa
      121 atggctgatt ccgctgctga ataccaccca acaaccggcg cttgccgaaa tgcgcgatcg
      181 tgcgacgcgt gaaaaactgt ttattgcggg ctggacgcga gcggaaaaaa atgatggcaa
      241 tgatacccgc gctatcattc aacgtctggt agagattcgc gcacagcagg cgaaactgct
      301 tggttttcct cattatgccg catggaaaat cgccgatcag atggcaaaaa cacctgaagc
      361 agcactcaac tttatgcggg aaattgttcc agcggcgcgt caacgtgcta gcgatgaatt
      421 agcctccata catgcggtta tcgataagca gcaaggcggg tttagcgcgc agccgtggga
      481 ctgggcattt tatgccgaac aggtacggcg ggagaaattt gatcttgatg aggcgcagct
      541 caagccatat tttgaattaa acacggtgtt gaatgaaggt gtattctgga ccgcgaatca
      601 gctcttcggt attaagtttg tcgaacgttt tgatattcct gtctaccatc ctgacgttcg
      661 tgtgtgggaa atttttgatc ataatggcgt gggactggcg ttattttacg gtgatttctt
      721 cgcccgtgat tcaaaaagcg gcggtgcatg gatgggcaat tttgttgagc aatcaacgct
      781 taatgaaacg catccggtaa tttataacgt ttgcaattat cagaaacccg ctgccggtga
      841 gcctgcgttg ttactctggg atgatgtcat aaccttattc catgaatttg gtcatacgct
      901 gcacggcctt tttgcccgcc agcgttatgc cacgctttcc ggcaccaaca cgccgcgtga
      961 ttttgtcgaa tttccgtcgc aaatcaacga acactgggca acgcatccgc aggtattcgc
     1021 tcgctacgcc cggcattatc agagcggggc agcaatgcct gacgaactgc aacagaaaat
     1081 gcgtaatgcc agcctgttca acaaagggta tgagatgagc gaactgctta gcgccgcact
     1141 tctcgatatg cgctggcatt gcctggaaga aaacgaagca atgcaggatg tcgatgattt
     1201 tgaattgcgg gcgctggtgg cggaaaatat ggatcttcct gctataccgc cacgctatcg
     1261 cagcagttat ttcgcccata tttttggtgg cggatatgcc gcaggttatt acgcttatct
     1321 gtggacacaa atgttggccg atgatggtta tcagtggttt gttgagcagg gcggattaac
     1381 gcgtgaaaat gggctgcgtt ttcgcgaggc gatcctttcc agaggtaaca gcgaagatct
     1441 ggaacgcctg tatcgacaat ggcgcggtaa ggctcctcag attatgccga tgctgcaaca
     1501 tcgtggcttg aacatataaa caccaccaat ggctgtcctg gagatatcgc caggccagcc
     1561 tggttatagc agtaattgca gcagctgggc gagggcaaac ctcaccgcta aagctaatac
     1621 cgtttcgcag tcgccagcaa aatgcataac cgcagtatag gtctggcctt taatatgcca
     1681 cgcgaaccag acggtacccg ctggcgtacc atcttcaccg ccctccggtc cgccgtagcc
     1741 ggtaatggca atactgacat cagcatccgc acgctctatg gcaccggttg ccatttctgc
     1801 tgccactttc tcactcaccg cagaatatcg ttcaagagat tgctggctta cgctgaggat
     1861 tttcatcttt gcctgatcgg tgaaagtaac aaagcctgca ccgtaaaatt tgggtgtatc
     1921 ttcagctgca cacagggcgc tagccaactt accgccggtg catgattcgg cggtagttaa
     1981 gcgtaattga tccgcgataa gtctttgact caacgcggat gtcaggtttt caatagtgtc
     2041 ggtatctgca agttgtacaa ttttatcgct attaatgttc actgtttttt gtccacgctt
     2101 ttatatgtaa aggtgatttg ctttttctgt atgctcgtcc aatataataa ttttcaaaca
     2161 tataaatccg att
//
LOCUS       DAFNBJ010000316         2149 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.324, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000316 DAFNBJ010000000
VERSION     DAFNBJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.324"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            129..767
                     /locus_tag="L2X49_004945"
     CDS             129..767
                     /locus_tag="L2X49_004945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBN4799877.1"
                     /translation="MIPTKYDLHLFTDYMQPVNLSFNQYLLLTEEPVLFHTGSAQQAA
                     ALLPRLEAALAGRPLRHIFVSHFESDECGGLAALLDRYPDASVICPEVTARQLMGFGY
                     QCSLDAKKPGEHIVTDSFDLEFIGYPSEMHLWEGLLAYEHRRKIFFSSDLMMRWGETG
                     SDLVHSSWKDEVGGITAEQVPSPQGRLRLQEDLMRFSPELIATGHGPVVDLS"
     gene            857..1234
                     /locus_tag="L2X49_004946"
     CDS             857..1234
                     /locus_tag="L2X49_004946"
                     /inference="COORDINATES: protein motif:HMM:NF013412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBN4799878.1"
                     /translation="MLDKKLLEVLTSPPDAAVAIVTQGKDGPHVVNSWNSYAQITEDD
                     KILFPAGRMNETEQNIRANNRVKMTICNREVQGISYKGTGFLVIGTAEFAAKGPDYDT
                     VKAKFPWARAALAIKVESAEQTL"
     gene            complement(1527..>2149)
                     /gene="bla"
                     /locus_tag="L2X49_004947"
     CDS             complement(1527..>2149)
                     /gene="bla"
                     /locus_tag="L2X49_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HBN4799879.1"
                     /translation="RVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAA
                     ITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPA
                     AMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGS
                     RGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
BASE COUNT          507 a          536 c          595 g          511 t
ORIGIN      
        1 attcttcgtg aaatagtgat ttttgaagct aataaaaaac acacgtggaa tttaggtata
       61 ttgcatatcc gccccgtggg caaggtcaag ttttaacagg gtattaccat taaaaaggag
      121 aagagacaat gattccaaca aagtatgatc tgcatctgtt taccgattat atgcagccgg
      181 tcaacctgtc ctttaaccag tatttgcttt tgacggagga gcccgtcctg ttccatacgg
      241 gcagcgccca gcaggcggcg gccctcctgc cgcggctgga ggcggctctc gcgggccggc
      301 cgctccggca tatctttgtc tcgcattttg agtcagatga atgcggcgga ttagcggcgc
      361 tgctggacag gtacccggac gcctcggtca tctgccccga ggttaccgcg cgtcagctga
      421 tgggctttgg atatcagtgc agcctggacg cgaaaaaacc gggggagcat attgtaacgg
      481 attcctttga cctggagttt atcggatatc cctctgaaat gcacctttgg gaggggcttt
      541 tggcctacga gcacaggcgc aaaatcttct tcagcagcga tttgatgatg cgctggggcg
      601 aaacgggcag cgacctcgtt cactcaagct ggaaggatga ggtgggcggc atcacggccg
      661 aacaggttcc gagcccgcag gggcggctcc gcctgcagga ggatctcatg cgcttttctc
      721 cggaattgat cgcgacggga catggcccgg tcgtcgattt aagctaatgg tgtttttccg
      781 cctatcctaa aacagtttgt ctccccgcac cgggcagggg gacctaaatt gtatattgaa
      841 aggagtgtct ccaccgatgt tagacaaaaa gcttttagag gtgctcacct cgccgccgga
      901 cgcggcagtc gccatcgtta cccagggcaa ggacggcccg cacgttgtaa actcctggaa
      961 cagctatgca cagataaccg aggatgacaa gatccttttc cccgcgggcc ggatgaacga
     1021 aaccgaacag aacattcggg ccaacaacag ggtgaagatg acgatatgca accgggaagt
     1081 gcagggaata agctataagg gtacaggatt tcttgtgatt ggcacggcgg agtttgcggc
     1141 caaggggccg gactacgaca cggtcaaggc aaaattcccg tgggcacggg cggccctggc
     1201 gattaaggtc gaatccgcgg agcagacgct atagcgtaaa aagcggccgg gaaaaaagcg
     1261 atttccaaaa gggtgacggg gtaaaaaccc gtcacccttt tgtgcagagc ctgtgcaggg
     1321 tgggtcttac gggccctgcg ccttttgtaa agctatgctt ccgctatggg tgcgcctagt
     1381 cagggcagcc gggcgggctg ttcgccgaaa ctcgctatca gtttaaattc ttcgtgaaat
     1441 agtgattttt gaagctaata aaaaatgcac gtggaattta ggtttaaatc aatctaaagt
     1501 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1561 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1621 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     1681 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1741 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1801 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     1861 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1921 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     1981 agtaagttgg cagcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2041 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2101 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggg
//
LOCUS       DAFNBJ010000317         2137 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.325, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000317 DAFNBJ010000000
VERSION     DAFNBJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.325"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(61..1107)
                     /gene="aroH"
                     /locus_tag="L2X49_004948"
     CDS             complement(61..1107)
                     /gene="aroH"
                     /locus_tag="L2X49_004948"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBN4799880.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYYEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1264..2097)
                     /gene="ppsR"
                     /locus_tag="L2X49_004949"
     CDS             complement(1264..2097)
                     /gene="ppsR"
                     /locus_tag="L2X49_004949"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBN4799881.1"
                     /translation="MENAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT          520 a          537 c          558 g          522 t
ORIGIN      
        1 tgtgctgaga agacaacaag ttgcgcagaa atgcccatcc catcaggaat gggcacgcat
       61 tcagaagcgg gtatctaccg cagaggcgag tttttcgacc aggcgttcgg tatcttccca
      121 gcccagacac gggtcggtaa tggattgacc gtaagtgagc ggctgaccgc cgacgatttt
      181 ttgcgttcct tcgcgcagga aactttccgc cataattcca gcaatcgccg tagagccatt
      241 gcggatttgc tgacaaatat cctcacaaac ttctaactgg cgacggtgct gcttctggca
      301 gttaccgtgg ctgaaatcca ccaccagatg ttcaggtaaa tcaaactcgt gcagcgtatc
      361 gcaggctgcg gcgatatcat cggcatgata attcggtttt ttgccgccac gcataataat
      421 gtggccatac gggttgccgc tggtctgata gatggtcatc tgaccatttt tgtctggcga
      481 gagaaacata tggctggcgc gggctgcgcg gatagcatcc acggcaatcc gcgtgttgcc
      541 atcggtacca tttttaaaac ctaccggaca ggagagtgcc gaagccattt cacggtggat
      601 ctgactttcg gtagtacgtg cgccaatcgc tccccaactg attaaatcag caataaactg
      661 accggtcacc atatcgagga actcagtcgc ggtagggaca cccagctcat ttacctgtaa
      721 aagtaatttg cgcgccagct ccagaccgtg atttacccga tagctgccgt ttaaatctgg
      781 atcggagatt agccctttcc agccgacaac agttcggggt ttttcataat aggtgcgcat
      841 tacgatttca agccgtgact ggtactggtt gcgcagcgac tgcagacggg tggcgtattc
      901 cattgcagcg gtgagatcgt ggatcgagca ggggccaata atgaccaaca gtcgcttatc
      961 ttcaccattc agtatttttt caattctgcg gcgggagtcg gtgacatggg tggcgacgcc
     1021 aggcgttacg ggataccgta gcgcgagttc ggcgggcgtt accaggctct caatacgcgc
     1081 agtacggagt tcgtcagttc tgttcattac aagtctcaga aatttgttgc ttctacggca
     1141 ggactaccgg gaagtgatgc gcatcacgat aaaccaatcg cagctgattt caagtgcgag
     1201 gtgtgtcaca cctcgccggg tggttagcat gataacaaaa aaacaagtta atgcactagt
     1261 tctctagtac attcgacggc taaggcccat gatatcgagg atcttggtgg caatctcttc
     1321 taccgaataa ttggtactgt taatccacgg gatctgattt ttacggtaca acgcttccac
     1381 ttccgcgact tccatcctgc actgacgaag cgaggcatag cgactgttct cccgacgttc
     1441 ctcgcgaatc gccgccagtc gttccgggtc gatagtcagg ccgaacaatt tatgctgaag
     1501 cggtttgagc gacgcgggta gcaccagatt atccatatcg tcggcaataa aggggtagtt
     1561 tgccgcgcgg ataccaaact gcattgccag atacagactg gtgggggttt taccacagcg
     1621 agaaacaccg agcaggatca cctgagcctg gtccagattg cgcaacgaaa tgccgtcatc
     1681 gtgggcgagg gtgtaatcaa tcgccgcaat gcgcgcatca tatttattga gattattagg
     1741 gttaaggcca tgggtacgat gagcaatcgg cgttggatcc agtttcatct cttgttgtag
     1801 cggggcaacc agcgcctgaa cgatatcctg gcaaaaacct tcactttgca agatgatggc
     1861 gcgaatctcc ggcaacacga tggagtagaa gaccagcggg cgcacacctg tctggtgata
     1921 aattgcgtca atctgatcct tcactgcccg tgcacggctc tcattttcga caaacggcag
     1981 cgtaatgctg ctgatagtga cgggaaattg tgacattact gcgtgtccta atacctccgc
     2041 agttattgcc gtaccatcag aaatataaaa aacgtggcga tcaacagcat tttccatttt
     2101 gttcttcccg tgatgcagac ataattctat gaaagca
//
LOCUS       DAFNBJ010000318         2098 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.326, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000318 DAFNBJ010000000
VERSION     DAFNBJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.326"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..415
                     /locus_tag="L2X49_004950"
     CDS             <1..415
                     /locus_tag="L2X49_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799882.1"
                     /translation="YIPENLYIIGTMNIADRSLALLDLALRRRFAFIDLKPAFNDAWR
                     NWVNYNYAIDFDMLAFIKSRLTVLNDMLAKDVTLGPQFCIGHSYVTPAIGQKINDAQA
                     WYEQVVDTEICPLLAEYWFDAPNKVDEARKVLLAK"
     gene            412..1533
                     /gene="mcrC"
                     /locus_tag="L2X49_004951"
     CDS             412..1533
                     /gene="mcrC"
                     /locus_tag="L2X49_004951"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HBN4799883.1"
                     /translation="MTTITGSEQHTASRIPMRNIWMLMLYASDLFRQLGRNQIAVEDN
                     PAEIPDLVATILLHEITLRRRRNLNMGYQTCHAALNRVRGRIDILYTASHQLLERGQV
                     ACHFQDITLNTPRNRYVRCALERLASIIVKPSLAADCHFMAISLRREGINGGYPAPHE
                     LPSVWRFGRHDAADKPMVDAARLAFELLMPTEDQGQYLLPAPSNNLYWMRRLFEKGIA
                     GLYRVHLAKTTWQVSAGKELKWALSDQSAGSAEIFPTMKSDIILEHKTAQRRIIIDTK
                     FNSILTKGWHREQSLRNGYIYQLYTYLRTQENKTDPLSLNAAGLLLHPAVGYMLNEYV
                     VTQGHKIHFATVDMTVDAKAIKKQLLAIAHDCAGVAVDD"
BASE COUNT          606 a          456 c          463 g          573 t
ORIGIN      
        1 atacattcct gagaatctat atatcatcgg tacgatgaat attgcagacc gctcactcgc
       61 actacttgac ctagcgctgc gccgtcgttt cgcgtttatc gatctaaagc cagcttttaa
      121 tgacgcgtgg agaaactggg taaattataa ttacgcaatt gattttgaca tgttagcatt
      181 catcaaatct cgactgacgg tacttaacga tatgctggca aaagatgtta ccttaggacc
      241 acagttttgt atcggtcaca gctatgtcac cccagctatc ggccaaaaaa tcaacgatgc
      301 ccaggcttgg tatgaacaag tcgttgatac agagatctgc cctttgctcg ccgaatattg
      361 gttcgatgct ccaaataaag tcgatgaagc cagaaaagtg ttattagcaa aatgacgaca
      421 ataaccggga gtgagcaaca tacagcatca cgtattccca tgcgaaatat ctggatgctg
      481 atgctgtacg cttcagatct gtttcgtcag ttagggcgca atcagatcgc agtagaagat
      541 aacccagcag aaatccccga cctggtggcg acaatcctcc ttcatgagat tacgctgcgc
      601 cgacgtcgca atttaaatat gggataccaa acttgtcatg cggccctgaa ccgagtgaga
      661 gggcgcattg acatactgta tactgccagt catcaactac tggaacgggg acaggttgcc
      721 tgccattttc aagacataac cctcaacacc ccccgaaatc gctatgttcg ctgtgcgtta
      781 gaacgtctgg cctcaatcat agtgaaaccg tctttggcgg cagattgcca ttttatggca
      841 atatcacttc gccgtgaggg gataaatgga ggatatcctg ccccccacga attaccctca
      901 gtgtggcgtt ttggacgcca tgatgccgca gataaaccga tggtcgatgc cgcacgatta
      961 gcttttgaac tgctgatgcc gaccgaagat caaggccaat atcttttacc tgcgccctca
     1021 aataaccttt actggatgag aagactattt gaaaaaggta tcgctggctt atatcgtgta
     1081 caccttgcca aaacaacttg gcaggtctct gcagggaaag agctgaaatg ggcgttgagc
     1141 gatcaaagtg caggtagtgc agagattttc ccgacaatga agtcagacat tattctggaa
     1201 cacaagactg cccagcggcg tattattatc gataccaaat ttaattcgat tctgacaaag
     1261 ggttggcatc gggagcaatc actacgtaat ggctatattt atcagctata tacctacctt
     1321 agaacacagg agaacaagac ggatccgctt tcgctcaatg ccgctggtct tctacttcat
     1381 ccagctgttg gctatatgct taatgagtat gttgtcactc agggacataa gatccatttt
     1441 gctacagtag atatgacggt agatgctaaa gcaattaaaa aacaattgct ggcaatagcc
     1501 catgattgtg caggagtcgc ggttgatgac taaccatcct ataaggcact ggctgtaata
     1561 taatcaatag tttcaattaa gtagctgtta tccgtgctta gtcatcatat gacgcaccac
     1621 ggtctaattt tcctgctcat acattgatat atccttgtcg agtccggaaa gtcgcagtcc
     1681 actattaggt ataccgattg ccagcaccaa aagcttcttt gtgattatta tatcggggaa
     1741 tgcctatttt ttagtgacaa tatcagatag caattaataa gcggtgccgg taatggatgt
     1801 tatgtttaat gtatagacac agccatcata aactaaacgt ctgttgttcg ctcataaccg
     1861 acatttatct cagttatgga agataattat gtatgcagat aatggggcag acagtcgtgg
     1921 agtaaacaag gttttgcact gcttggcggc atcgttatct tatttttaat tatgtgcctc
     1981 ttaacataag ttgggacaaa acagagacac ataaggtctc acaaaggtct gcaaggcctc
     2041 acatgtattg agatgttaat aaaatgtaga cttgtaattt ggatataaat ggtagaga
//
LOCUS       DAFNBJ010000319         2086 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.327, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000319 DAFNBJ010000000
VERSION     DAFNBJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.327"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            193..1077
                     /gene="yhdJ"
                     /locus_tag="L2X49_004952"
     CDS             193..1077
                     /gene="yhdJ"
                     /locus_tag="L2X49_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBN4799884.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NRSKRCRVSEVDPDLIAK"
     gene            1161..1340
                     /locus_tag="L2X49_004953"
     CDS             1161..1340
                     /locus_tag="L2X49_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBN4799885.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(1343..2005)
                     /gene="acrS"
                     /locus_tag="L2X49_004954"
     CDS             complement(1343..2005)
                     /gene="acrS"
                     /locus_tag="L2X49_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HBN4799886.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          598 a          457 c          453 g          578 t
ORIGIN      
        1 aataaatatc tctttccgca tcaattattt atgatgaata caaattatct agagtgataa
       61 taaattacgt ataatgactc tgtattttac aatacggcat gaactaattc aggttagcta
      121 aatgcttgat taaaaaggcg ctactcggca tggggaagcg ccttttttat aggtgtcaca
      181 aagggagtga ccatgagaac aggatgtgaa ccgacccggt ttggtaatga agctaagacc
      241 attattcacg gtgatgccct tgccgaactt aaaaagctac ctactgaaag cgtcgatctg
      301 atctttgccg acccaccgta taacatcggt aaaaattttg atggtctgat cgaagcctgg
      361 aaagaagatc tgtttatcga ctggctgttt gaagtgattg cagagtgcca ccgcgttctg
      421 aaaaagcagg gcagcatgta cattatgaac agtacggaaa acatgccctt tatcgatctc
      481 cagtgccgca agctttttac catcaaaagt cgcatcgtct ggtcatatga cagttctgga
      541 gtacaggcga aaaaacacta cggctccatg tacgaaccca tcctgatgat ggtgaaagac
      601 gcaaagaact acacattcaa cggtgatgct attctggtag aagccaaaac cggatcgcag
      661 cgcgcgttga tcgattatcg caaaaatcct ccacagccat acaatcatca aaaagtaccg
      721 ggtaacgtct gggattttcc gcgcgtgcgt tatttaatgg atgaatatga gaaccacccg
      781 acgcaaaaac cggaagcctt actgaaacgc attattctcg cctcttccaa cccaggcgat
      841 atcgttctcg acccgtttgc cggtagcttt actaccggtg ccgtagccat cgccagcgga
      901 cgaaaattca ttggtattga gatcaacagc gagtacatca aaatggggct tcgacggctg
      961 gatgtcgcgt cgcattactc cgcggaagaa ctggcgaaag tgaaaaaaag aaagacgggc
     1021 aaccggtcaa aacgatgccg ggttagcgaa gttgaccccg atctcattgc aaagtaaagg
     1081 aagtgtaagc ctggttttca gattattcat ttcgtgtata tttcctgcca gacttggtta
     1141 aacatgcaca ggctcaggta atgattcgca agtattggtg gctcgtcgtt ttcgctgtct
     1201 tcgtttttct gtttgatact ttactgatgc agtggattga actgctggca acagaaacag
     1261 acaaatgccg caatatgaac tcagttaatc cactaaaact ggttaactgt gacgaactga
     1321 attttcagga cagaatgtga atttacatga cacttaattc attcgtttga tgaattaatt
     1381 tcgttatgtt ttcatctggc atgaacattc ttaatacgtt atcgaccagc gcgggtgctt
     1441 gtttataaag atcataaccc gccatattca ttaaccagtt ttgaacaatt ccgctgaagg
     1501 caccatcaat aataatcatc acaacatcta aatcgaggtt atttgctaca caaccttgtt
     1561 gctgacacgc ctgcaatact tcgcggagag tctgcggatt aaagcccatc ttttcgcgta
     1621 tcactccctc ggccagcatc tcatcattaa attcacattt gtgatataag attttcagca
     1681 acgcctgctg gcggggaatt ttggcaatat attgcaagcc gacaatcaat ttttcacgca
     1741 attgttgaaa cgggtcatgc tctaatccag ccgtcaagtg ttcctggatt aactcccgca
     1801 atgaaggctg ttgcaaccac atctcattaa acagttgagt cttgttttcg aagtgccagt
     1861 agatagcgcc acgcgtaacg ttagcagcgt cggcaatgtc gttgagtgtc gtcttgctta
     1921 cgccatgctg cgcaaactgg gcgatggcag tttcaatcag ttcttgccgg gtcttcagag
     1981 cttcggcttt ggttcttttt gccatgatta attattcagg aaataaatat attcgacaca
     2041 gagtgagaaa atagcgaagg ttaatctatc acctaatgtg tattta
//
LOCUS       DAFNBJ010000320         2051 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.328, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000320 DAFNBJ010000000
VERSION     DAFNBJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.328"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            115..870
                     /locus_tag="L2X49_004955"
     CDS             115..870
                     /locus_tag="L2X49_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1405)-N(7))-methyltransferase
                     RmtB1"
                     /protein_id="HBN4799887.1"
                     /translation="MNINDALTSILASKKYRALCPDTVRRILTEEWGRHKSPKQTVEA
                     ARTRLHGICGAYVTPESLKAAAAALSAGDVKKALSLHASTKERLAELDTLYDFIFSAE
                     TPRRVLDIACGLNPLALYERGIASVWGCDIHQGLGDVITPFAREKDWDFTFALQDVLC
                     APPAEAGDLALIFKLLPLLEREQAGSAMALLQSLNTPRMAVSFPTRSLGGRGKGMEAN
                     YAAWFEGGLPAEFEIEDKKTIGTELIYLIKKNG"
     gene            complement(951..1499)
                     /locus_tag="L2X49_004956"
     CDS             complement(951..1499)
                     /locus_tag="L2X49_004956"
                     /inference="COORDINATES: protein motif:HMM:NF013188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBN4799888.1"
                     /translation="MRDTIKVLLLLGASFALVALEKTLGERALFSGLLAVMGMGVTLL
                     KTNAPVAKRISGKFSKLLVAAEIWLFVLVGATVNIRYLFSAGLSGMLLITAALLFRML
                     GVWMSTLGTDLSRKERLFCMIAYLPKATVQAAIGAIPLAMGLGSGETILAVAVLAIIL
                     TAPLGALGIELSYKRLLQKQQS"
     gene            1520..1912
                     /locus_tag="L2X49_004957"
     CDS             1520..1912
                     /locus_tag="L2X49_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799889.1"
                     /translation="MPAGKVLCTDLPWQGINEDQNLGIAITRRALEAPLRAIVANAGE
                     EPSVIVANVKAGEGSYGYNAATGEFGDMIAMGILDPTKVTRSALQHAASVAGLAITTE
                     VVVAEVPKKEEPAMPGAGGMGGMGGMDF"
BASE COUNT          467 a          616 c          573 g          395 t
ORIGIN      
        1 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
       61 gtgagttttc gttccactga gcgtcagacc ccgaaaacga tttaggagac accgatgaac
      121 atcaacgatg ccctcacctc catcctggcc tcaaaaaaat accgcgccct ttgcccggat
      181 accgtgcggc gcatcctgac tgaggaatgg gggcggcata aatcccccaa acagaccgta
      241 gaggctgcac gcacccggct gcatggaatt tgcggggcat atgtcacccc ggaatcgctc
      301 aaggctgctg ccgccgcgct ttctgcgggc gatgtaaaaa aggcattgtc gctgcatgcc
      361 tccaccaagg agcgactggc cgagctggat accctgtacg attttatctt ttcagccgaa
      421 actccccgcc gcgtgctgga tatcgcctgc ggtcttaacc ccttggcgct atacgagcgc
      481 ggcattgcat ccgtgtgggg ctgtgatatc caccagggat tgggggatgt catcaccccc
      541 tttgctaggg aaaaagattg ggattttacc tttgccctgc aggatgtgct gtgtgcgccg
      601 cccgccgaag ccggcgacct ggcgctgatt tttaagcttt tgcccctgct ggagcgggag
      661 caggccggtt ctgccatggc acttttacaa tccctcaata ccccgcgcat ggctgtcagc
      721 tttcccacgc gtagtttagg cgggcgtgga aaaggcatgg aggcgaacta cgccgcatgg
      781 ttcgagggcg gcttgcccgc cgagtttgag attgaggata aaaagaccat cggaacagaa
      841 cttatatact tgataaaaaa gaatggataa gccaatcaga aggcaaatca tctaaaaaag
      901 aggaacgaaa aatatttttc gttcctcttt tgttctatcg aacaagcaca ctacgactgc
      961 tgtttttgaa gcagcctttt atagctcagc tcgatgccca acgcccccag cggcgctgta
     1021 agtataatgg caagaaccgc caccgcaagt atggtttccc cactgccaag ccccatcgcc
     1081 agcggaatag cgccgattgc agcctgcacc gttgccttgg gcagataggc aatcatgcag
     1141 aacaggcgct ccttccggga taggtctgta cccagcgtac tcatccacac gcccagcatg
     1201 cgaaacagca gcgcagctgt gatcagcagc atgccgctta gcccggcgga gaaaaggtaa
     1261 cggatgttga ccgttgcacc caccagcaca aacagccaga tttctgccgc caccaaaagc
     1321 tttgaaaatt tgccggatat acgctttgca accggggcgt tggtttttaa aagggtgacg
     1381 cccatcccca tcaccgccag caatccggaa aatagcgccc tttcgcccag ggttttctcc
     1441 aacgcaacca gcgcaaagga tgcgcccagc agcaggagca ccttgatcgt atcacgcata
     1501 tgtacccgct taaaaagggc tgccggccgg caaggtgctg tgcacggatc tgccctggca
     1561 gggcatcaac gaagaccaga acctgggcat cgccatcacc cgccgtgcgc tggaagcccc
     1621 gctgcgcgcc atcgtggcca acgccggtga agaaccgagc gtgatcgtgg ccaacgtcaa
     1681 ggccggcgaa ggcagctacg gctacaacgc cgccaccggc gagttcggcg acatgatcgc
     1741 catgggcatc ctggacccga ccaaggtgac ccgctcggcc ctgcagcacg ccgcttccgt
     1801 cgccggcctt gcgatcacga ccgaagtggt cgtggccgaa gtgccgaaga aggaagagcc
     1861 ggccatgccg ggtgctggcg gtatgggcgg catgggcggc atggatttct gatccggttg
     1921 gcccggtcgt cagggaaccg gaccgcgcca gcgcggtccg atcccggcaa cgacccgaca
     1981 tcaaggcccc aaggacgggg ccggagcccg gcagcgatgc cgggcttttt gttgtgcccg
     2041 cgccgcggca a
//
LOCUS       DAFNBJ010000321         1982 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.329, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000321 DAFNBJ010000000
VERSION     DAFNBJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.329"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..1430
                     /locus_tag="L2X49_004958"
     CDS             <1..1430
                     /locus_tag="L2X49_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBN4799890.1"
                     /translation="PDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYH
                     YDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTW
                     YGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGE
                     DGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARK
                     IHLYHCDHRGLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGG
                     YGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKW
                     VKPNLTCTPQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGP
                     ATAGIDDLGEILAKMKQRTSRGIRK"
     gene            1427..>1982
                     /locus_tag="L2X49_004959"
     CDS             1427..>1982
                     /locus_tag="L2X49_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799891.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIR"
BASE COUNT          573 a          464 c          520 g          425 t
ORIGIN      
        1 ggccggataa ccgtatcgcc cgtgacgcgc actatcttta ccggtatgac cgtcacggca
       61 ggctgacgga gaaaaccgac ctcatcccgg aaggggttat ccgcacggat gatgagcgca
      121 cccaccggta ccattacgac agtcagcacc ggctggtgca ctacacgcgg acacaatatg
      181 cagagccgct ggtcgaaagc cgctatcttt acgacccgct gggccgcagg gtggcaaaac
      241 gggtgtggcg acgtgaacgg gacctgacgg gctggatgtc gctgtcacgg aaaccgcaag
      301 tgacctggta cggctgggac ggcgaccgcc tgaccacaat acagaacgac agaacccgca
      361 tccagacgat ttatcagccg gggagcttca cgccactcat cagggttgaa accgccaccg
      421 gtgagcaggc gaaaacgcag cgccgcagcc tggcggatac ccttcagcag tccggcggcg
      481 aagacggtgg cagtgtggtg ttcccgccgg tgctggtgca gatgctcgac cggctggaaa
      541 gtgaaatcct ggctgaccgg gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc
      601 tgacggtgga gcagatgcaa aaccagatgg acccggtgta cacgccggcg cgaaaaatcc
      661 acctgtacca ctgcgaccat cgcggcctgc cgctggcgct tgtcagcacg gaaggggcaa
      721 cagaatggtg cgcagaatac gatgaatggg gcaacctgct gaatgaagag aacccgcatc
      781 agctgcagca gcttatccgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt
      841 acaaccgcca ccgctattat gacccgctgc aggggaggta tatcactcag gatccgattg
      901 ggctgaaggg gggatggaat ttttatcagt atccgctgaa tccggttcag tatatagatt
      961 caatgggact ggcatcaaaa tatggacact taaataatgg cggatatgga gcgagaccca
     1021 acaaaccgcc tacgcccgat ccaagtaaat tgccggacat agcgaaacaa ttaagactgc
     1081 catatcctat tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat
     1141 taagccctta cgactacaca ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta
     1201 cgccacagga tgattcccag tatccaggga tggatacaaa gacagcaagt gattacctgc
     1261 cacagacaaa ttggccaaca actcaattac caccaggata tacttgtgca gaaccctatt
     1321 tattcccaga cattaataaa cccgatgggc cagcaacagc agggatagat gatttgggtg
     1381 aaattttagc taaaatgaaa cagagaacat cgagaggaat aagaaaatga aaagagtttt
     1441 gttctttttg ctgatgatat ttgttagttt tggtgttata gctgattgcg aaatacaagc
     1501 taaagatcat gattgtttta ctattttcgc taagggtact attttttccg cgtttcctgt
     1561 cttaaataat aaagccatgt ggagatggta tcagaatgag gacatcggtg agtattattg
     1621 gcaaacagaa ctgggtacat gtaaaaataa taaattcaca ccaagcgggg cgagactctt
     1681 aattcgcgtc gggtcactac gccttaatga aaatcatgcc atcaaaggaa cgttgcagga
     1741 gttgataaat acagcagaaa aaacggcgtt tctcggcgat cggtttagaa gttatataag
     1801 agcgggtatc tatcaaaaaa agagcagtga tccagttcag ttgttggcag tccttgataa
     1861 ttcaatcatg gtaaaatatt ttaaagatga gaaaccaact tatgctcgaa tgacagcaca
     1921 tctcccgaac aaaaatgagt cttacgaatg tttaataaag atacagcatg aactaattcg
     1981 ta
//
LOCUS       DAFNBJ010000322         1938 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.330, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000322 DAFNBJ010000000
VERSION     DAFNBJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.330"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            384..509
                     /locus_tag="L2X49_004960"
                     /pseudo
     CDS             384..509
                     /locus_tag="L2X49_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            1225..1794
                     /locus_tag="L2X49_004961"
     CDS             1225..1794
                     /locus_tag="L2X49_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBN4799892.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKVHCTGERNFGCSRG"
BASE COUNT          484 a          509 c          518 g          427 t
ORIGIN      
        1 cagagagtaa cgtaaaggat ttgccagagt aagctaagag agtaaatctc tccggtaaca
       61 gagcgctgtg aatcgttcat gtatcagagt gcagatacat gaacgctgat gcgtaaaatc
      121 agcagtgaca cgccagctcc ccctgtgccc attcctgtat aagacatgaa ctgagcgtat
      181 taatgctcct gccctgagac tgagccggtg agtgaggggc tgagaaatcc ctcttgacac
      241 tgctctaaac cagaaatact tccccggttg ttgcggtgcg tccggatccc cgataacgga
      301 acactaaggc tgtattgcgg gacacctggt aagtggcagc ccgtaatacg tcaggagacg
      361 cgggaaaagt gaattcccac acgcgttata aacttaccct tgcggagctg gaagccttta
      421 actctgccgt tgacaaccgg ctggcagaac tgacaatgaa caaactttac gatcgcgcgc
      481 cggcttccgt ctggaaatat gtcacctgaa tattgaagca gcgctacgat gactccggtg
      541 ttatctgcac cggaggaccg ggaacattaa gcggtatagc gacaacaaag aattcgaaat
      601 caaaagggaa tggtgccgga ggcgtgggcg gtaataggct gattttcagg tattaccggc
      661 ctgtatctgc tgaagggggc tcgtatttgt cggggctgcg tattccgctc gattcgatca
      721 cggattggcg gaatacctaa ctgtgattga ttcaggtacc attttgcagg gatccggtgc
      781 gttactgtct gacacaacaa acgcagcggg tcgctctgct ccgccgattc ctgataatcc
      841 ctccccagta acctactgca acagtacaaa tacactgacg cctgaaacgg taaccagaat
      901 cagcaccagc agtaactcag taaacagagc agacgatcgg tcttccggta ataaccggaa
      961 ggaacgacag cgccacaata caggcaacac actgtattat gagtaatcga attaccgcaa
     1021 tccccgcatt tctttttgcg tatcacgata gctccctcgc cacgccttat ccgtaaccgg
     1081 ttttttacat taaaaaaaac aacgccggga attcaatcgt cagtccggag acgaccgttc
     1141 gggttatcac agagagcctg agcagggggg ctgcgcattt tttacgatgt ggctattccg
     1201 tatgaaccat acggagatat aaccatgacc tacaaataca accccttctg gcagcaacgt
     1261 attcgtgaga cggtgcggca cgcactgaat gttcatcccc gcctgacggc attgcgggtt
     1321 gacctgcgtt tcccggatgt accggcagca acggacgcag ctgtgatatc ccgcttcatc
     1381 aatgccctga aagcccgaat cgacgcttac cagaaacgta agcatcggga aggtaaacgc
     1441 gtgcatccca caaccctgca ttacgtctgg gcccgggagt ttggggagtg caaaggtaaa
     1501 aaacactatc acctgatgct gctggtcaac cgggatacct ggtgtcgtgc cggtgattac
     1561 cgcgctccgg gatcactggc cgggatgatt aaacaggcgt ggtgcagtgc cctgggagtg
     1621 gatgtcgggt gccatgccac gctggtgcat tttccggcct ggccggcggt gtggctggcg
     1681 cgtaatgatg acaccggctt tcagcaagtg ctggaacgtg ctgactatct ggcgaaggag
     1741 cataccaaag ttcactgcac cggtgagcgc aactttggct gcagtcgtgg ctgagccaga
     1801 cagacgagtt atctgacttc agtgtgatga cgtgttcacg gccaccacac tgattttccc
     1861 ttatgagact gaactgaaac cgccattcac accggaccac gctgcccgta ccgggacggc
     1921 tgttgcctgc gtgtcgtc
//
LOCUS       DAFNBJ010000323         1936 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.331, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000323 DAFNBJ010000000
VERSION     DAFNBJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.331"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            345..1742
                     /locus_tag="L2X49_004962"
     CDS             345..1742
                     /locus_tag="L2X49_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBN4799893.1"
                     /translation="MAIHDAGMFTVKEINRLKILQDVVERNLRPGQAAEMLGITPRHC
                     SRLLKRYRQSGPLGMNNQSRGRTGNRLLPTSLTDQALSIIRERYRDFGPTLAREKLEE
                     VHGLVLGKETIRRLMIKAGLWIPRRQRAPKIHQPRYRRPCTGELIQIDGCDHHWFENR
                     GRPCTALVYVDDATSRLMHLLFVKSESTFSYFEATRGYIEKYGKPMILYSDKASVFRV
                     NNKHATTGPGETQFARAMRCLNITPLCAETSQAKGRVERAHLTLQDRLVKELRLKGIS
                     TIEAANAFAEEYMADYNRRFAKAPLHDFNAHRPLALDDDLDAEFTWREPRRVSKSLTV
                     QYDKMLYLIEDSEYSRRAIGKYIDVWHYPDGHKELRLNGVLLPYSTYDRLSEVDPVAI
                     VDNKRLGHVLDVARQVQKKRDNNRSQSLPSGDEPSRRRHAPGINKSQRSLNEDDLLEA
                     IIKLQGSSEAIFGKR"
BASE COUNT          531 a          500 c          469 g          436 t
ORIGIN      
        1 accaatgtcc aaagtcgccg gttaggagca acgctcaact ggtcaagtac atacatctga
       61 gacatcctca gagaggttcg tggatatgaa gaaaatagct cttaaacata acgccagcaa
      121 actaaagatt aagaggggct taaccctatg attttatgcg agccatctaa gttcatgaag
      181 taagttaaca atcaacatga tttaacataa tccatcttat gcgcactaag tctgtagagg
      241 ctcaatgcta atgtctattt gtggctcatt aggaatgtcc aggctatagt tttcaaacct
      301 gtatgcctag tcaacgactc gcttttcctg atgaggagtt ttctatggct atacatgacg
      361 ccgggatgtt caccgtgaaa gaaatcaacc gcctcaaaat tcttcaggat gtcgttgaac
      421 gtaacctgcg tcctggccag gctgccgaga tgctcggcat cacgccacgt cattgcagcc
      481 gtctgctgaa gcgttatcgc cagtccgggc cgcttggtat gaacaaccag agtcgcggtc
      541 gcactggcaa ccgcctgttg cccacatcac tgactgacca ggcactgagt atcatacgag
      601 aacgctaccg cgattttggc cccacgctgg cccgggaaaa actggaggaa gttcacggtc
      661 tggtcctggg caaggaaacc atcagacgcc ttatgataaa agccggactc tggatccccc
      721 gaagacaacg cgcaccgaaa attcatcagc cccgttaccg gcggccctgt actggcgagc
      781 ttatacagat tgacggctgt gaccatcact ggtttgaaaa ccggggtcgg ccttgtacgg
      841 cacttgtata tgtcgacgat gccaccagtc gtctgatgca cctgctgttt gttaaatctg
      901 agtccacctt tagctatttt gaagcgaccc gtggctatat cgagaaatac ggtaagccca
      961 tgatccttta cagcgataaa gccagcgtat tcagagtcaa taataagcac gctaccactg
     1021 gtcccggtga aacccagttt gcccgagcca tgcgctgcct caacattacc ccgctatgtg
     1081 cggagaccag ccaggcaaag ggccgtgtgg aacgcgccca cctgacactt caggaccggc
     1141 tggttaagga gctcaggctt aaagggatca gtacgataga agccgcgaat gcatttgctg
     1201 aggaatacat ggcggattat aaccggcgct tcgccaaagc ccccctgcat gattttaacg
     1261 cacatcggcc gctggcatta gacgatgacc tggatgcgga atttacctgg cgagagcccc
     1321 gtcgtgtatc aaaaagtctg accgtacaat atgacaaaat gctctatctg attgaagaca
     1381 gcgaatacag tagaagggct atcggcaagt atattgatgt ctggcactat cctgacggac
     1441 ataaggaact gcggttgaat ggcgtgctac tgccctactc cacctacgac cgattgtctg
     1501 aagtcgatcc tgtcgcgatt gttgataaca aaagactggg ccatgtattg gatgttgccc
     1561 gccaggtcca gaaaaaaagg gacaataatc gctcccagtc gctaccttca ggagatgagc
     1621 cttcccgcag acgacatgct ccaggcatca acaaatccca gcgctcgctg aacgaagatg
     1681 acctgcttga agccataata aagcttcaag gaagctctga ggccatcttt ggtaaaaggt
     1741 gaacttgtcg gaaacctcgc actcactgac cagcagcgct gtgttgcagt taatgcaatc
     1801 aaagcctgaa aaaaattctg agaaatgcaa ataacttcaa tgatattata tacaaagaga
     1861 aacgtcgggt agccggggta aagtccgggt gtcacagaac cccgaaccgc ttcgcggtga
     1921 cttcttacca caaacc
//
LOCUS       DAFNBJ010000324         1921 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.332, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000324 DAFNBJ010000000
VERSION     DAFNBJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.332"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            45..1322
                     /locus_tag="L2X49_004963"
     CDS             45..1322
                     /locus_tag="L2X49_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005183576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBN4799894.1"
                     /translation="MQKITHQQLLDWLKKEVKLSLGCTEPIAIAYAAAVAAKYLNAPI
                     LKITGNISENLYKNAMGVTIPGTSYCGVTLAAAIGAIGGNADADLEVLKDITATQITE
                     AYIFNESGNVCLNAVDATDFIFIDITLYSLSDQCRVVIQGSHTNVTEVYINNIKQILV
                     RDLIAANDSGVLPEFSLNDAFKFVTDVAAKDIMFMLKSAEINTALSDEGQRKNYGLNV
                     SGALSQARKNGFISTDLLSQMLINTTAASDARMGGAPLPAMSNYGSGNQGITVTMPVV
                     TLARHLNTDDETLARALALAHLAAISIHMRYTRLSALCAASTAAMGAAAGMSWLLTQD
                     FQTISYAVSNMISDISGIICDGASNSCAMKVSTATACAFKSVLMAQQNSVAGERDGIV
                     SCDVEGSINNLCKLVLSPMRQTDKEIISIMTRK"
     gene            1404..>1921
                     /locus_tag="L2X49_004964"
     CDS             1404..>1921
                     /locus_tag="L2X49_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004711301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HBN4799895.1"
                     /translation="MSKSKSIATLVIHAGDQVDRGNNAIFPPITTASSFIQPNLGEEG
                     EFCYSRCSNPTRYAYETALAELEGGIYATATASGMAATALILELLPKDSHVLVMKGVY
                     GGTYRLFERLRKQTSGTTFSYVDLNDLASVERHIQENTGLIWIETPTNPLLELVDIEA
                     VCQVAKSRGIMS"
BASE COUNT          569 a          375 c          449 g          528 t
ORIGIN      
        1 accctaagat aaaaataatc caaatcactg attaattact tcaaatgcaa aaaataacac
       61 atcaacaatt actcgattgg ttgaaaaagg aagtgaaact ttcattaggt tgtactgagc
      121 ctattgctat tgcgtatgct gccgcagtgg ctgctaaata tctcaacgca ccgatattaa
      181 aaataacggg gaatatatcg gagaatctgt ataaaaatgc gatgggagtc actattccgg
      241 gaacaagcta ttgcggagtc actcttgcgg cggcaatagg ggctattggt ggtaacgcgg
      301 atgccgattt agaagtatta aaagatatta ctgcgacaca aattaccgaa gcctatatat
      361 ttaatgaatc aggtaacgtg tgtctaaacg cagtagatgc tacggatttt atttttatcg
      421 atattacgct atatagtctt agtgatcagt gccgggtagt gattcaaggt agccatacta
      481 atgtcactga agtttatatt aataatataa agcaaatatt agtccgagac ctaattgccg
      541 ccaatgatag tggggtacta cccgaatttt cccttaacga cgcatttaaa tttgtaaccg
      601 atgtcgccgc taaagacatc atgtttatgc tgaaaagtgc tgagattaat accgcgcttt
      661 ctgacgaggg gcaaaggaag aactatggtt tgaacgtgag tggcgcgttg tcgcaagcca
      721 gaaaaaatgg ctttatcagc actgacttac tgagtcaaat gttgatcaac accactgcgg
      781 catcagatgc ccgtatgggc ggcgcaccct tacccgcgat gtcaaattat ggctctggta
      841 atcaggggat taccgtgaca atgccggtag tgacacttgc ccgacatctt aatacagatg
      901 atgaaacact ggcccgggca ttagcgttgg cccatctagc cgcaatctca attcatatgc
      961 gttatacccg attgtctgca ttgtgtgcgg cgtcaacggc agcaatgggg gcggcggcag
     1021 gtatgagttg gttattgacc caagattttc aaactatctc ctacgcggtc agtaatatga
     1081 tcagtgatat tagtggcatt atttgcgatg gtgcatcaaa tagttgtgcc atgaaagtgt
     1141 ctaccgctac cgcctgtgca tttaagtcgg tgttgatggc gcaacaaaat agtgttgcag
     1201 gggagcgtga tggcattgtt tcctgcgatg ttgaaggttc tatcaataat ttatgtaaat
     1261 tagtgttatc gccaatgagg caaacagata aagaaattat cagtataatg acacgtaaat
     1321 aaatattacg gtttaaccgc gtggcctaac gtcattatta ataatgatcg gcgggctatc
     1381 gttaaaattt aaagggacaa ataatgagca aatcaaaatc gatcgcgact ctggttatcc
     1441 atgccggaga tcaagttgac cggggcaata atgctatttt tccaccgatt actaccgcca
     1501 gttccttcat tcagccaaat ttaggcgaag aaggggagtt ttgttattcc cgttgcagta
     1561 acccgacaag atatgcatat gaaaccgcct tggctgagtt ggaaggaggt atctatgcta
     1621 cggcaaccgc ttccggtatg gcagcaactg cacttatatt ggaattgcta cctaaggatt
     1681 cccatgtatt agtaatgaaa ggggtctatg gcggtactta tcggttgttt gaacgtctgc
     1741 gtaaacaaac ttcaggtacg acattcagtt atgtcgatct taatgatctg gcgtcagtag
     1801 agcgccatat tcaagagaat acaggtctaa tctggatcga aacgcccact aacccactgt
     1861 tggaattagt tgatattgaa gcagtatgcc aggttgccaa aagcaggggc ataatgagct
     1921 g
//
LOCUS       DAFNBJ010000325         1894 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.333, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000325 DAFNBJ010000000
VERSION     DAFNBJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.333"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..1894
                     /locus_tag="L2X49_004965"
                     /pseudo
     CDS             1..1894
                     /locus_tag="L2X49_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
BASE COUNT          664 a          368 c          353 g          509 t
ORIGIN      
        1 attatatcaa cttttcacgc agaaaaagtt attaatgatc tgttaaaaaa cgattataaa
       61 atttcccttt ctccagatac aacttatcga gagttgaata aagcagcaca gcgtagcatt
      121 acagcgccag acaggatagg agaaggaaaa tcatgggttt atcaaagaga tacaatgatt
      181 gaaagaggtg ataacagcgg tgttcatcag tatggtcctg ctgaacattt cacccacatt
      241 atatctgaca aaccgtcccc aaaagatgag tatattgcat atgctattaa cattcctgac
      301 tatgagctgg cagccgatgt atataatatt aacgtgacgt caccttccga acagcaagaa
      361 acatttaaaa tactaatcaa tccagaacat ctacggcaaa cacttgagcg taaatctctt
      421 actgctgttc agaaatcaca atgtgaaatc ataaccccca aaaaacctgg cgaagcgatt
      481 cttcatgctt ttaatgccac ctaccagcaa atcagggaaa atatgtctga gtttgcacgt
      541 tgccattatg ggtatataca aatccctcca gtgacaactt tccgcgccga cggaccagaa
      601 actcccgaag aagaaaaggg ttactggttt catgcttatc aacccgaaga tctttgtacc
      661 atccataatc caatgggaga tttgcaggat tttattgcat tggttaaaga tgctaaaaaa
      721 tttggtatcg atatcattcc tgattatacc tttaacttta tgggaattgg gggtagtggt
      781 aaaaatgacc tggattatcc ctctgctgat atacgagcga agatcagtaa agatatagaa
      841 ggtggtatcc ctggctattg gcaaggtcag gttttgattc cattcattaa agatccagta
      901 acaaaagaac gtaaacaaat ccatccagaa gatatacatc tcactgcaaa agacttcgaa
      961 gcaagtaaag ataacatctc taaggatgaa tgggaaaacc tccatgcatt aaaagaaaag
     1021 cgtttaaatg gaatgcctaa aacaacaccc aaaagtgacc aggttattat gttgcaaaat
     1081 caatacgttc gtgaaatgcg aaaatatggc gtacgaggtt tacgttatga tgcggcaaaa
     1141 cactcaaaac atgaacaaat agaaagatca ataaccccac cgcttaaaaa ttataatgag
     1201 cggttacaca atactaactt atttaaccca aaatatcata aaaaagccgt tatgaattac
     1261 atggaatatc tggtaacttg tcagttggat gaacaacaaa tgtcatcact gctttatgaa
     1321 agagatgatt taagcgccat tgatttttca ttgctcatga aaacgataaa agccttttca
     1381 tttggtggag atcttcaaac ccttgcatca aaaccgggtt ccacaatctc aagtatccca
     1441 tcagaaagac ggatattgat taacattaac cacgattttc ctaacaatgg taatcttttc
     1501 aatgactttc tatttaacca tcaacaagat gaacaattag caatggcata tatagccgct
     1561 ctgccgttca gcaggccttt agtttactgg gatggccaag tattaaaatc aacgactgaa
     1621 attaaaaatt atgatgggtc cacgcgtgtc ggcggtgagg cgtagcttaa taaaggttgc
     1681 tctacctatc agcagctcta caatgaattc cacgcattat atatagataa agcaggaata
     1741 tggagcgcat ttgagggtgt atctgcaact aaaaacgttc tggcctttag tcgtggggat
     1801 tctgtgaaca ttaatcactc tcctcatgat ggactagtta taataaataa aggaaacgaa
     1861 gaagttgaag gtacctggcc taacaaattg caac
//
LOCUS       DAFNBJ010000326         1891 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.334, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000326 DAFNBJ010000000
VERSION     DAFNBJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.334"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            42..1688
                     /gene="mqo"
                     /locus_tag="L2X49_004966"
     CDS             42..1688
                     /gene="mqo"
                     /locus_tag="L2X49_004966"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HBN4799896.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPKPVQKEVADIAL"
BASE COUNT          456 a          464 c          565 g          406 t
ORIGIN      
        1 ctcacctgca ataaagcgac taaaagtaag gcattaacaa gatgaaaaaa gtgactgcca
       61 tgctcttctc gatggccgtg gggcttaatg ccgtttcgat ggcggcaaaa gcgaaagcgt
      121 ctgaggagca ggaaactgat gtactgttga ttggcggcgg cattatgagc gccacgttgg
      181 ggacctattt acgcgagctg gagcctgaat ggtcgatgac catggtggag cgcctggagg
      241 gtgtcgcgca ggagagttcg aacggctgga ataacgccgg aaccgggcat tctgcactga
      301 tggaactgaa ctacaccccg caaaacgccg atggcagcat cagtattgaa aaagcagtcg
      361 ccattaacga agcatttcag atttcccgcc agttctgggc gcaccaggtt gagcgcggcg
      421 tgctgcgtac tccgcgttca tttatcaata ccgttccgca tatgagcttt gtctggggcg
      481 aggataacgt taatttcctg cgcgcccgtt acgccgcgtt gcaacaaagc tcgctgtttc
      541 gcggtatgcg ttactctgaa gatcacgcgc agatcaaaga gtgggcaccg ttagtgatgg
      601 aagggcgcga tccgcaacag aaagtggcag ccacgcgtac ggaaattggt accgatgtga
      661 actacggcga gatcacccgc cagttaattg cttccttgca gaagaaatct aacttctcgc
      721 tgcaactcag cagcgaagtc cgcgccctaa agcgtaatga cgataacacc tggaccgtta
      781 ccgttgccga tctgaaaaat ggcactgcac agaacattcg tgccaaattt gtctttatcg
      841 gcgcgggcgg tgcggcgctg aagctgttac aggaatcggg gattccggaa gcgaaagact
      901 acgccggttt cccggtgggc ggacagttcc ttgtttcgga aaacccggac gtggttaatc
      961 accatctggc gaaggtttac ggtaaagcat ccgttggcgc accaccgatg tcggttccgc
     1021 atatcgatac ccgcgttctg gacggtaaac gcgtagtgct gtttgggcca tttgccacct
     1081 tctcaaccaa attcctcaaa aacggttcat tgtgggatct gatgagttcc accaccacct
     1141 ctaacgtgat gccgatgatg cacgtcgggc tggataattt cgatctggtg aaatatctgg
     1201 tgagtcaggt gatgttgagt gaagaggatc gttttgaagc gttgaaagag tactatccgc
     1261 aggcgaaaaa agaggactgg cgtttgtggc aagcggggca gcgcgtgcag attatcaagc
     1321 gtgatgccga gaaaggtggc gtactgcgtc tgggtactga agtcgtcagt gaccagcaag
     1381 ggaccattgc cgcgctcctg ggggcatcgc caggggcgtc aaccgccgcg ccgattatgt
     1441 tggatctgct ggaaaaagta tttggcgatc gcgtttccag cccgcaatgg caggctacgt
     1501 tgaaagcgat cgttccgtct tatggacgca agctgaacgg tgatgtagcg gcaacagaac
     1561 gcgagttgca gtacaccagc gaagtgctgg ggctgaaata cgacaagccg caggcagcag
     1621 atagtacgcc gaaaccgcag ttgaaaccga aacccgttca aaaagaagtg gcggatattg
     1681 cgttgtaatg atgtgccaca tccggcatgg tatgccgggg atgtggcgta tgctgataag
     1741 acgcgccagc gtcttatcag gcaaccggct cgggcgttag atgtcggatg cggcgtaacc
     1801 gccttatcca atctacgggg tgcagcactt cacagcggtg taggcctgat aagacgcgcc
     1861 agcgtcgcat caggcaaccg gcttgggcgt t
//
LOCUS       DAFNBJ010000327         1874 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.335, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000327 DAFNBJ010000000
VERSION     DAFNBJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.335"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            350..1837
                     /gene="ltrA"
                     /locus_tag="L2X49_004967"
     CDS             350..1837
                     /gene="ltrA"
                     /locus_tag="L2X49_004967"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBN4799897.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRR
                     TQSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRW
                     MDYAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALV
                     KILNAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHE
                     WLIRFTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYV
                     FDLWAHQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEK
                     TRLMEFGRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLK
                     AIKDGLRRRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRR
                     RSQKDDTTWTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
BASE COUNT          482 a          502 c          586 g          304 t
ORIGIN      
        1 cggtagagat caaggggtga aagtccccga ccattgaagg accagcaatc cacaaggtcc
       61 ccgagtcatg cgttgcatac cgcgaggtat ggggcgaagc gttgacaggg gtgttgacag
      121 gccagccatt gagccacgaa atgtatatta aattaccggg tgccgacgtt gtactgttaa
      181 cggaaggcaa catcataggg tgcgatactg cgagtgccac atggacccgg cggggtctga
      241 gactctggca tgtcaatacg atctctacgc gggaaccggg agatctcccc tctgaccatc
      301 tgctagtgtc ggagatggcc cgcaccggga agacgaggag tcatagccgg tgatgtacgg
      361 agaggagaag tcggactcgc tcatagtagc ggcgaagcag gcgaacaacc cgaaaggagc
      421 ggagtcagtg gagcgaagga gcggggccaa ggggaacgcg gaacagccac acatgcgccg
      481 gacacagagc cgggaaagca tgtcacagag gctgtcacgc gtgcgggaag ctgcgaagca
      541 gcggaagaaa gaacggttta cagcattgtt ccacctgctg acagtcgaag cactggaagc
      601 cgcattcctc tccctgagca ggaaagcggc cgccggagtg gatggcatca ggtggatgga
      661 ctacgccgga aacatgaaga acaacataac agatctgcac cggaggctac atcagggcag
      721 ctacagggcg cagcccggca ggcgtcacta catcccaaaa gcggatggaa aacaacgccc
      781 gctcggcatc gcctcgctgg aggacaagat cgtccagtat gcgctggtga aaatcctgaa
      841 cgcagtctat gaaaacgact ttatggggtt ctcatacggg ttcagacccg ggcgaagcca
      901 gcacgatgca ctggacgcac tggccacagg gctggtacgc actaacgtaa actgggtact
      961 ggatgccgac atcagtcagt tcttcgacag ggtgagccac gaatggctga tcaggttcac
     1021 agagcatcgg atcggcgacc ggagggtaat caggctcata cgtaagtggc tcacagccgg
     1081 gacgtcggag gagggtcaat ggcgagcaac ggaggaaggc accccacagg gtgcggtcat
     1141 ctcaccgctg ctggcaaaca tatacctcca ctacgtcttc gatctgtggg cgcatcagtg
     1201 gcgacgtcgc tatgccacag gcaatgtggt aatggtcaga tacgccgatg acatcgtcat
     1261 cgggttcgac aaacgatacg atgcccggcg cttccgtata gccatgcagc gcagactgag
     1321 ggagttcgga ctcacggttc acccggagaa aacccgtctg atggagttcg gccgcttcgc
     1381 tgccgaaaac cgtgccatca ggggaaaagg caaaccagaa acgttcaact tcctcgggtt
     1441 cacgcacatc agcgggaaag atcgcaacgg caggttcatg ctgatacgaa agacccgccg
     1501 ggatcggatg acggcaactc tgaaagccat caaagacggt ctgcgaaggc gctggcatta
     1561 ctcaatcccc gaacagggaa aatggctcag gagagtggtt cagggatacc tgaactatca
     1621 ctcggtaccg ggcaacttcc ccaccatgca gaagttcagg acacacgtaa caaacctctg
     1681 gcgccgggcg ctcaggcgca ggagccagaa ggatgatacg acctggacga aagcaaacaa
     1741 actggcagcc gcatggctac caagggttcg ggttcttcat ccatggcctg tggagcggtt
     1801 caccgccaga cacccgaggc aggagcccgg tgcgtaaatc gcgcacgccg ggatctgtgc
     1861 ggggggtatc cggt
//
LOCUS       DAFNBJ010000328         1851 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.336, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000328 DAFNBJ010000000
VERSION     DAFNBJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.336"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     repeat_region   214..730
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=275..303
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaactc"
     gene            complement(1070..1741)
                     /gene="queE"
                     /locus_tag="L2X49_004968"
     CDS             complement(1070..1741)
                     /gene="queE"
                     /locus_tag="L2X49_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBN4799898.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          491 a          454 c          406 g          500 t
ORIGIN      
        1 ttaattaata ttaaaaaaat aatatattaa aatttcttaa cggtttaagg attatacaaa
       61 taaaactttg attaaatttg attttttgta tcgataattg tgaacttatc tggcatgggg
      121 aactattttg aacatgaggt gttacgtgga tatgttgctt attacaagta cagctaatat
      181 aaaaacttga gaaagagata acgggttata tggtggttta tccccgctgg cgcggggaac
      241 tcatatacag caaaccttca gaatgcattc cgggcggttt atccccgctg gcgcggggaa
      301 ctcgccgtct gcctccagcc cagccctgga tatttcggtt tatccccgct ggcgcgggga
      361 actctggttc actcacgcaa cgtctgagcg cgttgacggt ttatccccgc tggcgcgggg
      421 aactcagggc cgccgctacc ccagaagtgc cactccccgg tttatccccg ctggcgcggg
      481 gaactcgcgt atcgtctcgt tattgcgccg ccccaactcg gtttatcccc gctggcgcgg
      541 ggaactcagt atgcgttcaa tcactgtcag gcctacttcc ggtttatccc cgctggcgcg
      601 gggaactcag ttgcccgatg gacactccca gaccatcagc cggtttatcc ccgctggcgc
      661 ggggaactca ctaaacttaa tgatggccgt tacagcgtgg acggtttatc cccgctggcg
      721 cggggaactc tgtaaacata acctattatt aattaatgat tttttaagcc agtcacaatc
      781 taccaacttt acagtatcac acaaacaaca catccattat gttaaagagt actttattca
      841 ttgattaaaa aggtaaatat ttaaaataac tctatacaaa ttaaaatcta ccaaacttta
      901 ccgcaataat tttcactcca gcgaaaaatt aatgccacag aatttgtaga gaatgctaat
      961 gattagccct ggatgggtaa aatggaggag ttttcagagg cgacaaacaa tataaatgag
     1021 tagaagtctt tacaggttcc acccatttat aacgcttata aatgtttaat caggcaatat
     1081 ttagatattt atgtgtttgc atcgacaaac gccaattacg cgcaatgcag gtttcaatgc
     1141 acaaacgtgt ggcatcatcc ttttggctaa tcggctgcag tgcaatgact cgcggtttat
     1201 catcggtcag cgttgccagt agttcatcca gtgcttcaat atcgcgtacg cgccccactg
     1261 gatgcttgat ttcgttggct cgctccagtg cctgtgacaa cacttcatag ccgccgcgca
     1321 tgttcagctt tggcgatacg gtaacccagg tattcggtgt gcagcgtacc tcatgagtac
     1381 cactggtttc gatctggcag ctaaaaccgt tcttttcgag cagatcagtc agtggcagca
     1441 aatcatgaat gcaaggctca ccacccgtaa tcaccacatg ccgcgcggtg tatccctggc
     1501 gactaatgac agccaacaag tcttcactgc tcgccgcccc ccacttatca ctctctttgg
     1561 tcttcgccag aatgctgaaa agggagactt cccgatcctc aagcttttcc caggtgtgtt
     1621 tggtgtcgca ccaggcacag ccaaccgggc atccctgtaa acgaataaaa atggcgggga
     1681 cgccggtaaa gtaaccctca ccttgcaggg tctggaacat ctcgttaatc gggtactgca
     1741 tagcattctc tgtgaagtgg ataattgtta attattgcag atcctgccac aacaatcatg
     1801 tcttattaac attctgttac aggcagattt aaagaaaaat gcgaaacata t
//
LOCUS       DAFNBJ010000329         1830 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.337, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000329 DAFNBJ010000000
VERSION     DAFNBJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.337"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            508..1791
                     /gene="ydfJ"
                     /locus_tag="L2X49_004969"
                     /pseudo
     CDS             508..1791
                     /gene="ydfJ"
                     /locus_tag="L2X49_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT          476 a          354 c          448 g          552 t
ORIGIN      
        1 actaaatttt caatcatata tggattttta tgctgatgaa gatgttttat ttggcgattc
       61 gtcaagcatc ttcacttaag taatcgtata ttttaagagt gcaaaagaga aagggacaac
      121 gttgctcgca ctctggatta aatatagggt gttgttaaac tgcgttgata cataattgta
      181 ccggatattg agtggtctga agtcccagtc attaagtaat cgtttcgtga tatgccgcag
      241 aaacgttgta tgcaataacg ttctgcggtt ggctgatgca ctttcgagag tgtattgaat
      301 aatttcaagc cgttattgtt tttacgcgta acaccatgaa aagactgctt atctgttggg
      361 gtattttttg gggcatatat gggacagaaa taggccacag atagatagta agatcgaact
      421 caggatgctt ttgaaaaaat gtaactatct gaaaaaaact agaaagcgcc aaggaaacca
      481 cagaatgaga aaaaacatct gtgaattatg gatttccagt tatattcgct cggcgcagcg
      541 ttagtgtttc atgaaatatt ttttcctgaa tcatcaacgg caatggcgtt aattttggca
      601 atgggaacct acggtgcagg ttatgtggcg cgtattgtcg gagcatttat tttcggcaaa
      661 atgggcgaca gaatagggcg taaaaaagtg ctctttatta ccatcaccat gatggggatc
      721 tgtactacct taattggtgt gttaccgacc tatgcacaga ttggtgtttt tgcccccatc
      781 ttgctggtga cgctgcgtat tattcagggg ttgggtgcag gtgcggaaat ttccggtgcc
      841 ggtacgatgc tggcggaata tgcaccaaaa ggtaagcgcg gaattatctc ctcatttgta
      901 gctatgggaa ctaactgcgg aaccttgagc gcaacggcaa tctgggcctt tatgttcttc
      961 attctcagta aagaggaact gctggcgtgg ggatggcgta taccgttcct ggcgagcgtt
     1021 gtcgtgatgg tctttgctat ctggttacgt atgaatctga aagaaagccc ggtctttgag
     1081 aaggttaatg acagcaacca acccacagca aaacctgcac ctgctggtag catgttccag
     1141 agcaaatcct tctggctggc aacagggctg cgttttggtc aggcgggtaa ctccgggtta
     1201 attcagactt tccttgcagg ctatttagtg cagacgttat tgtttaacaa agcaattcca
     1261 acagatgcat tgatgattag ttcgattctc ggctttatga ccattccgtt ccttggttgg
     1321 ttatccgata aaattggtcg ccggatcccg tatattatta tgaatacctc cgcgattgtg
     1381 ctggcatggc caatgctttc tatcattgta gataaaagct atgccccgag caccattatg
     1441 gttgcactga ttgtgattca taactgtgcg gtgctgggat tatttgctct ggaaaacatt
     1501 accatggcag aaatgttcgg ctgtaaaaac cgctttaccc ggatggctat ttctaaagaa
     1561 attagtggtc ttatcgcttc cggttttggt cctatcctgg cgggtatttt ctgcaccatg
     1621 acggaatcct ggtatccgat cgccattatg atcatggcat attcagtaat tggtttaatc
     1681 tctgcgctga aaatgccaga ggtgaaagac cgtgatttaa gtgcgctgga agacgctgcg
     1741 gaagatcaac cgcgtgttgt aagagctgcg caaccttcca gaagtctgta aatcctcaat
     1801 cccttctctt accggagagg ggatttttat
//
LOCUS       DAFNBJ010000330         1807 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.338, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000330 DAFNBJ010000000
VERSION     DAFNBJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.338"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..1435
                     /locus_tag="L2X49_004970"
     CDS             <1..1435
                     /locus_tag="L2X49_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003953979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBN4799899.1"
                     /translation="KLVVIVLDNCDKRTLSEQLLMFQAAQWLQNEFRSLIILPLREET
                     YDNYRDRPPLDTALKDMVFRIEPPLFNYVLHSRVQLVLKEMTKTSASNTLSYSLPNGF
                     NVEYSKDERSYYLTSIANSIFVHDAQIRRMIVGLSGRNIRRALEIFLEFCTSGHITEG
                     EFLKIRQSEGRYALPLHIVTRVLLRLNRRFYDSDNSYLKNIFSTEKDDERANHFTRFA
                     ILKWFYNNFGVSGPTGLKGYFPLFALKNGLVKFGFSPKVITREVDYLLQGQCLVSEDF
                     RETGITDEDLLRLSSAGFVHLDMLSNISYLAALAEDTLFPTEQIARRVSERMGSVQNQ
                     YNKEMVSANAKELVDFLVNQRETIASFSGEFISGDEYHGLTDIQPAIDAVKSFAEKIR
                     DPEWAEFEARYQSGDILEGTVRNCDHRYGVFVNFTGKITGLIHKSKLPAGFNRMNEYS
                     KGKVLWVRIENDFNAVEQKISLSLVDQ"
BASE COUNT          554 a          280 c          395 g          578 t
ORIGIN      
        1 taaattagtt gtaatagttc ttgataattg tgataaaaga actttgtctg aacagttgct
       61 aatgtttcag gctgctcaat ggctccaaaa tgagtttaga tcactaataa ttcttccctt
      121 gagagaagaa acttatgata attatagaga tcgtcctcct cttgacaccg ctttaaaaga
      181 tatggttttc cgtattgaac caccgttgtt taattatgtt cttcattcaa gagtgcagct
      241 tgttcttaaa gaaatgacaa aaactagtgc tagcaatact ctttcatatt cacttcctaa
      301 cggctttaat gttgaatata gcaaggacga acgttcatat tatttaacat caatcgcaaa
      361 ctctattttt gtacatgatg cacaaataag aaggatgatt gttggtcttt caggaagaaa
      421 tatacgaagg gctttggaaa ttttcttgga gttctgcact agtgggcata ttactgaagg
      481 tgagttttta aaaataaggc aaagtgaagg acgctatgcg cttcctttac atattgtgac
      541 cagagtgtta ttacgattaa atcgtcgatt ttatgatagc gataactcat accttaaaaa
      601 tatattttcg acggaaaaag atgatgagag ggctaatcat tttactaggt ttgcaatttt
      661 gaaatggttc tataataatt ttggggtaag tggtccaact ggacttaaag gatactttcc
      721 tttgtttgcg ttaaaaaacg gattagtcaa gtttggtttt tctccaaagg ttataactcg
      781 tgaggtcgat tatttacttc aggggcaatg tttagtttca gaggatttta gagagactgg
      841 aataactgat gaagacttac taagattatc ctcggctggg tttgtgcatt tggatatgct
      901 ctcaaatatt agctatctag ccgcgttagc agaagatact cttttcccta ctgagcaaat
      961 tgccagaaga gtctctgaaa ggatggggtc agttcaaaat cagtataata aagaaatggt
     1021 tagtgctaat gctaaggagt tagttgattt tcttgtaaat caacgtgaaa cgatagcaag
     1081 tttttcaggc gaatttattt ctggagatga atatcacggg ctaactgata tccaacctgc
     1141 aattgatgct gttaagtcat ttgcggaaaa gatacgcgat cctgaatggg cagagttcga
     1201 agctaggtat cagtcaggtg atatattaga aggtactgta cggaattgcg atcatagata
     1261 tggtgttttt gttaacttta caggaaaaat tacagggtta atacataaat caaaactgcc
     1321 tgcaggtttt aaccgtatga atgagtattc caaaggtaaa gtactatggg tgcgtataga
     1381 gaacgatttc aacgctgttg agcaaaagat tagtttatct cttgtggatc aatgaatggt
     1441 tcctgtcaag ttactcgaca tatagattga tgtcgactga tgaggtcaag ctgagattgt
     1501 cgatgtatag atagtcctgc ataactatgc tgcatgaaaa cgcatgatcg tttagggatc
     1561 ttttatgctg aggcccgtca taactggcgg gcttttgcct atgtcatgca ggtgcatgaa
     1621 aaacactaca taaagcgggc aggcgtggcg gggatacgag cgcgcgctca ttacttttat
     1681 aattctaggg ttataatacg gggctatctt tctaggagaa aacacatgtc cttagagttt
     1741 atcaaagaac agttgtcaga ttttatctca tctacagaac cagaagtaat ggctatacag
     1801 ggtgaat
//
LOCUS       DAFNBJ010000331         1784 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.339, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000331 DAFNBJ010000000
VERSION     DAFNBJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.339"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(268..1784)
                     /locus_tag="L2X49_004971"
                     /pseudo
     CDS             complement(268..1784)
                     /locus_tag="L2X49_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
BASE COUNT          326 a          523 c          561 g          374 t
ORIGIN      
        1 gcataaatga tttttctgat tcacttaatt tttcagtacc gttacataat ccgtgaccat
       61 atggtaatgg agatgtcgtt aaccatactt tattatacaa aattgttcca tcatacaacg
      121 ttagatatcc ttctctcagg ccttcagatg caagtatgga atgcaacata ggaattgctg
      181 taaagtgata taaatccata aataacccta ttctgtctat ttatattcat tttaggggag
      241 cccgcagaat tcggaaaaaa tcgtacgcta aggttttccg ggcatccgta agggccgaaa
      301 cttcccgtct tccagtctgc ggctctgccg ccagacgtaa tcgccggtta ggttgatgtg
      361 ctcccagccc agcggcgaca ggaattgcag cagctcgccg tccaccggct tgccggcctc
      421 gaccaacccc tgggtggcgc gttccaggta caccgtgttc cacagcacga tagccgccgt
      481 caccaggttg aggccgctgg cccggtagcg ctgctgctcg aagctccgat ccctgatttc
      541 cccaaggcgg ttgaagaaca ccgccctggc cagcgagttg cgcgcctcac ctttgttcag
      601 gccggcatgc acgcggcggc gcagttcaac actttgcagc cagtccagga tgaacagcgt
      661 gcgctcgatc cggcccagct cgcggcaggg ccacggccag tccgttctgg cgcgggtagc
      721 tgccgagctt gcgcagcatc agcgaggcgg tgacggtgcc ctgcttgatc gagctggcca
      781 ggcgcaggat gtcgtcccag tgggcacgca cgtgcttgat gttcagggtg ccgccgatca
      841 gcgggcgcaa cgtcgggtag gcttgcacgc cctgcggcac gtacagcttg gtttcgccga
      901 ggtcgcggat gcgcggcgcg aagcggaagc ctagcaggtg catcagggca aagacgtgat
      961 cggtgaagcc ggccgtgtcg gtgtagtgct cctcgatccg caggtcggac tcgtggtaca
     1021 gcaggccgtc gagcacatag gtggaatcgc ggacgccgac attcaccacg cgggtgctga
     1081 acggcgcgta ctggtcggag atatgggtat agaacagccg tcccggctcg ctaccgtact
     1141 tcgggttgac gtgcccggtg ctctcgcccc ggccacccgc gcggaagcgc tggccatcgg
     1201 aggatgaggt cgtgccgtcg ccccagtggg cggcaaaggc gtggcgatac tggtggttga
     1261 ccagctcggc caaggccgcc gaataggttt cgtcgcggat gtgccaggct tgcagccagg
     1321 acagcttggc gtaggtcagg ccggggctcg actcggccat cttggtcagc ccgaggttga
     1381 tcgcatcacc gaggattgcg gacagcagca acgtcctgtc tttggcctcg gccccgtcct
     1441 tcaagtgggt gaagtggcgg ctgaagcccg tccagtcgtc cacgtccatc agcagttcgg
     1501 tgatcttgat gcgcggcagt aactggctgg tttggtcgat cagcgcctgc gcccgatccg
     1561 gcaccgccgc atccagcggg gtgattttca gccctgactc ggtgaggatg gcatcgggca
     1621 gctcgttgtc cttggccagg cgggtgacgg tggccaactg ctcgtccagc agctgcaaac
     1681 gctcttccag gtactggtcg ctgttcgggt tgatcgccag gggcagggcc tgctcgcgct
     1741 tgagtgcggc gaacttctcg gccggcagca ggtagtcgtc gaag
//
LOCUS       DAFNBJ010000332         1779 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.340, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000332 DAFNBJ010000000
VERSION     DAFNBJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.340"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..70
                     /gene="ariR"
                     /locus_tag="L2X49_004972"
     CDS             <1..70
                     /gene="ariR"
                     /locus_tag="L2X49_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HBN4799900.1"
                     /translation="ILKADVIRKTLEIVLRYTADDM"
     gene            183..431
                     /locus_tag="L2X49_004973"
     CDS             183..431
                     /locus_tag="L2X49_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799901.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMNNTTLTDIIINDRESVLS"
     gene            763..>1779
                     /locus_tag="L2X49_004974"
     CDS             763..>1779
                     /locus_tag="L2X49_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HBN4799902.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGI"
BASE COUNT          494 a          372 c          379 g          534 t
ORIGIN      
        1 tattctcaaa gctgatgtga ttagaaaaac actggaaatc gtgttgcgat acacagctga
       61 tgatatgtaa cttctaatta ttaagtataa gtttatagaa aactcattca tcttattttt
      121 gtctgtcgct ttagactata cacagcataa ttttattagg ttaatatttc tacgaggctg
      181 acatgaataa ttcaatccca gagagattta tttttcaatg tgctttgttt aaaaatctcg
      241 agagggaggt gttcatgacg catgggtatg ttgatagtca tattattgat caggctttac
      301 gcttgcgttt aaaagatgaa accagtgtga ttctttctga tctctatctg caaatattgc
      361 agtacattga aatgaataat actacactaa cggatatcat tattaatgac agggaatccg
      421 tgctctctta gaacttgagt aaggttttag aataaacatc agctgtatca ccatgctgat
      481 acaaagtgag catttcaggc gttatgcttt cttattatgt ccgcaatatc aggtgtcaag
      541 aatggagagt tctcgctctc cattcttgac gcctgatatc ccgcctaact tattttgtac
      601 tgcctacatt atgctcaata attaatgggt tagatcaaga tggagaaaat tttatcttcg
      661 gcgtggatat ttcttatgct ctctaccatg ctctctgtag atgccagcgc gagcgaatga
      721 atggcatttt catgtattaa accctttggc cgggacaagc aaatgcgcaa tacactcata
      781 cccatcctcg ttgcgatatg cttgtttatt accggtgtcg ctattttgaa catccagctc
      841 tggtattccg caaaagcaga gtacctggcg ggagcaagat atgccgccaa caatatcaat
      901 catatacttg aagaagcgtc acaggcgact caaacagcgg ttaacattgc cgggaaggaa
      961 tgcgacctcg aggagcaata tcagcttggc actgaagcag ctctgaaacc tcacctgcgc
     1021 acaatcatca ttctcaaaca gggaatagtc tggtgtacat ccctgcctgg gaatcgggtc
     1081 ctgctgtctc gtattcctgt tgtcccggac agtaatttac tgttggctcc ggcaatcgac
     1141 accgttaata gattacctat cctgctctat cagagccaat ttgcagatac gcgcattttg
     1201 gttacgataa gcgatcagca tattcgtggg gcacttaatg tacccttgaa aggggtaagg
     1261 tatgtattac gcgtggcgga tgacattatt ggaccaacgg gtgatgtgat gacgcttaat
     1321 ggacattatc cctataccga gaaggttcac tccacaaaat atcatttcac tattatcttt
     1381 aacccgccgc cactctttag cttctacaga cttatcgata aaggctttgg gattttaata
     1441 tttattctgt taatcgcctg cgccgctgcc ttcctgcttg atagatattt caataaaagc
     1501 gcaacgcctg aagagatcct gcgacgggca ataaataatg gggagatcgt ccctttttac
     1561 caacctgtgg taaatggtcg ggaagggaca ttgcggggag ttgaggtgtt agcccgctgg
     1621 aaacaacctc acggtggata tatatcaccc gcggcattta ttccacttgc tgaaaaatcc
     1681 ggattaatcg ttccgcttac gcaaagcctg atgaatcagg ttgccagaca gatgaacgct
     1741 atcgcgagta aattgccgga aggttttcat attggaatt
//
LOCUS       DAFNBJ010000333         1758 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.341, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000333 DAFNBJ010000000
VERSION     DAFNBJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.341"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(106..1287)
                     /locus_tag="L2X49_004975"
     CDS             complement(106..1287)
                     /locus_tag="L2X49_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN4799903.1"
                     /translation="MNSKPVTRQFEDSDLHQELVTFEVPNNDLKELIFYFSHMKYHTA
                     KTYLQWLRSWNEWYQANAGKEGNEAWPASSLPVTQPPLLSYLDYLQGSLSHSSIKGCL
                     HALNSIHRKALDRPGIITSKVKSILASLEQAEAREQKVTRQATPFLVSDLKALIKAHG
                     TTQSVRKLRDLCIIWTGFETLLRSAELRRIRMQDLVLNEQTGSFTLTVYRTKSTVSTL
                     LTYHLTPHLTATLIRLMGMVKRDQQSHPKDYLFQAVNFQDSGYMPPGWGLRSKGNEIN
                     TLLKNHNMPYRPTRPPIGKNGKPIIVDDEGMLSKNTLLRAFEAFWDELHPQEAGTRCW
                     TGHSVRVGGAIELADAGYTHLQIMEMGNWSNAEMVSRYIRNIDAGKKAMTKFMREALD
                     E"
     gene            1502..1714
                     /locus_tag="L2X49_004976"
     CDS             1502..1714
                     /locus_tag="L2X49_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799904.1"
                     /translation="MSETKQAYLMKFRKCSSFDTLEKVFERLCEKNAGIASLEISGAY
                     DHRKAELTMKKLYDKVPASVWTLVRQ"
BASE COUNT          472 a          397 c          422 g          467 t
ORIGIN      
        1 taagttggag tcattaccag agtttattgt caaataaaaa ggcagatttc tctgccttaa
       61 tacacaatat acatagttca aggtactata tataacgcag gggcgctact catccagcgc
      121 ctcacgcatg aacttcgtca tcgctttctt acctgcgtca atgttacgga tatagcggga
      181 aaccatttct gcattgctcc agttacccat ctccatgatc tgcaagtgcg tgtacccggc
      241 atcagcaagt tcgatggcgc cgccaaccct aacgctgtga ccagtccagc atcgggtgcc
      301 cgcttcctgc ggatgaagtt catcccaaaa cgcttcaaag gcccggagca atgtgttttt
      361 actgagcata ccctcatcat cgacaataat gggctttcca tttttcccga tcggggggcg
      421 ggtaggtcgg taaggcatat tatgattttt cagcagcgta ttgatctcat tccctttgct
      481 ccggagaccc catccagggg gcatatatcc gctgtcctga aagttaactg cctggaagag
      541 ataatccttc ggatggcttt gctggtccct tttgaccata cccatcagcc tgattagggt
      601 ggcagtgaga tgaggggtca gatgataggt aagcagggta ctgacagtgg attttgtccg
      661 gtacaccgtc agggtaaaac tgcctgtctg ttcattcagc acaaggtcct gcatccgaat
      721 gcgccgaagt tctgctgagc gcaacagtgt ttcaaaacct gtccagataa tgcaaaggtc
      781 tcggagtttg cgcaccgact gtgtcgtacc gtgggcttta atcaaagctt tcaggtcgga
      841 gactaggaat ggcgttgcct gacgagtcac tttttgctct cgggcttcag cttgttcgag
      901 gcttgcaaga atactcttga ccttggatgt gatgatccca gggcgatcga gtgctttcct
      961 atggatactg ttcagcgcgt gcagacaacc tttaatgctg ctgtgtgaca gcgagccctg
     1021 aagatagtcc agataggaca acaacggggg ttgagtcacc gggagagaac tggcaggcca
     1081 ggcttcgttt ccctcctttc ctgcattagc ttggtaccac tcgttccagg aacgcagcca
     1141 ttgaaggtaa gttttagccg tgtggtattt catatgagaa aagtaaaaaa tgagctcctt
     1201 aaggtcatta ttgggcacct caaacgtgac cagttcctga tggaggtctg agtcttcaaa
     1261 ctgtctggtt actggcttgc tgttcatggt ggtcacctga atacactgtt tttatttaca
     1321 gctattttag cactgaggta tccacttatg aacaggttca tagcaactaa aaaagagaga
     1381 gcgagtacaa caacgatccg gagataaaca tcaaatattt gtgaaaaaaa ttagattcta
     1441 gggacaaaac gacataagat tgggaaaccg tactttcgcg ggaaataaat taaggttgac
     1501 catgtccgaa actaagcagg cgtatttaat gaaatttcgt aagtgctctt ctttcgacac
     1561 actggaaaaa gtattcgaac gactctgtga aaaaaatgca ggtatagcat cactggaaat
     1621 ttccggggca tatgaccatc gaaaagctga actgacaatg aagaaactct acgataaagt
     1681 acccgcaagc gtatggacac tggtccgtca gtaatatgaa gatattggta accaatttat
     1741 cgccgacttt tcaggaaa
//
LOCUS       DAFNBJ010000334         1659 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.343, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000334 DAFNBJ010000000
VERSION     DAFNBJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.343"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..611
                     /locus_tag="L2X49_004977"
     CDS             <1..611
                     /locus_tag="L2X49_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HBN4799905.1"
                     /translation="YQEEKKTPIIMVKKRRTLLPPSLSEKTGVIARSETVKMEDPVSV
                     GSSSPAVDTHPPEASASKKKRKRRRFPRQPHWTHEFTHNCVEKVKSLFPHLRAESGGF
                     LPLKIGITNDFAAFLTEHPETELTLDEWSCAISCITTRQVYLLRTAVAGIPRYGLDGL
                     PAGQVSECDAQNARRWLAVREKQKLKMKTMQEQTASTEKIER"
     gene            complement(670..1125)
                     /locus_tag="L2X49_004978"
     CDS             complement(670..1125)
                     /locus_tag="L2X49_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799906.1"
                     /translation="MRFAEYPWTERKLYWLNEGGSHHFAAARYQACRLGISVPLTGRL
                     SRFHVNMQMVSALCQQWHLFAIPADERLACFFRAMIAFECPFGNSELPRNMHNTIKSG
                     VKLKLVWLERGHTKADIVADVLATAGFPDFGDQLKLLATSSLQKTHKLA"
BASE COUNT          492 a          394 c          352 g          421 t
ORIGIN      
        1 aatatcagga agaaaagaaa acacccatca ttatggtgaa aaaacgtcgc actcttttac
       61 ccccttcctt atctgaaaaa acaggtgtta tcgcacgttc agagacggta aagatggaag
      121 atccagtatc tgtgggaagc agttctcctg ccgtcgatac tcatccccct gaagcttctg
      181 ccagtaagaa aaaaagaaaa agacgccgtt ttccacgcca gcctcattgg actcatgaat
      241 ttactcacaa ttgcgtggaa aaagttaagt ctctgtttcc ccatcttcgc gcagaaagcg
      301 ggggatttct tccactgaaa attggcataa ccaatgattt tgccgcattc ctgacagaac
      361 accctgagac agaactgacc ctggatgaat ggtcctgtgc aatatcctgc atcaccaccc
      421 gacaggtata tctgctgcgt actgctgtcg caggtatccc tcgttatgga ctggatggac
      481 taccagcagg tcaggtatca gaatgcgatg cgcagaatgc cagacgctgg ctggcagtaa
      541 gggaaaaaca gaagctaaag atgaaaacca tgcaggaaca gaccgcgtca accgaaaaaa
      601 ttgagcggta atttcatcac gataacagca ggttaccaac acaatgatat tcattgtgaa
      661 gtattccttt caggccagct tatgtgtttt ttgcaggctg ctggtagcca gcagcttcaa
      721 ttgatcccca aaatcaggga aacctgctgt ggcaagtaca tctgcaacaa tgtctgcttt
      781 ggtatgacca cgttcaagcc aaacgagttt gagtttgact cctgacttta tagtattgtg
      841 catattccga ggtaattctg aattaccaaa tgggcattca aaggcaatca tcgccctgaa
      901 aaagcaggct aaccgttcat ctgcaggaat agcaaacagg tgccattgct gacataaagc
      961 tgaaaccatc tgcatgttta catggaagcg actcaatctg cccgtaagcg gaacagaaat
     1021 tcccagacgg catgcctgat aacgggcagc tgcaaaatga tgtgagccac cttcattcag
     1081 ccaatacagc ttacgctcag tccacggata ttcagcaaag cgcattcgat ggagccttaa
     1141 ctcccagtac tgcatatttt ctctgagatg ttcttcattt accggggtaa ccagcccagg
     1201 gcatctcagt agaggaaatt cataaatgtc accaatatca tacatctcag atttcgatgc
     1261 ggagataccc tgaatatcgg tgatatcaca cgaaaaatga ggaatatctt ctttgctaat
     1321 cagattctga acttcgggaa caaccatatg acatcgttct aaaccacgat agtagcctgg
     1381 cttatagaaa tctatcgtac attggcgaga atcagatggt atccatgatg gggatatcca
     1441 gtcctgccag taaatcaccg atcgcgtcca tagtgtatcg acaggagttt ttaaaggaca
     1501 cgggattaac tccccggatg caatcgctct ttcaagttgc cctggtcgcc ccagagaatg
     1561 tcttattttt tcatagcccc atccaactat actgcgcaaa acagaaagtt gagttttatc
     1621 tgacagctcg gaagatatgt atctctcttt tttatcatt
//
LOCUS       DAFNBJ010000335         1624 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.344, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000335 DAFNBJ010000000
VERSION     DAFNBJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.344"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(89..481)
                     /locus_tag="L2X49_004979"
     CDS             complement(89..481)
                     /locus_tag="L2X49_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799907.1"
                     /translation="MKYILTVLCIFFCQTASAYPYRIYTAPEGAIVKNILTNEVIGKT
                     PVEVDVSNTEAGSTFGISMYRHENVAIKIFTVMPNAENNFTVSGPDVATMSLPGKAPL
                     NVTNDSNAAAVHIDLRPFLSEPAHHPVQ"
     gene            complement(478..999)
                     /locus_tag="L2X49_004980"
     CDS             complement(478..999)
                     /locus_tag="L2X49_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799908.1"
                     /translation="MIRVSAFILTLLVTGCQSVGSKIAVLPSVGFDPIMSNRTEAYTD
                     GKVTFLIESSGTDVWLLAKNGTKEFIELSDLNLGGSRCTYSSRGKQLISPSSVTIFTV
                     PTVGLLGLCYDNNDQLTFINNSFKNISQSSRDGLTLPLLFSIKYKFPGSFDSKQIVVT
                     QSFDLEFLQKEQS"
     gene            complement(992..>1624)
                     /locus_tag="L2X49_004981"
     CDS             complement(992..>1624)
                     /locus_tag="L2X49_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799909.1"
                     /translation="SMTKRNYLQIENDSASTPLNLVIPKIEFSIIAKNGVFISKFISL
                     DEDASGKRILWLEPGSSMTISFVNDLSSTPLQALVSVTKQKKEVIAIFGPDQGKKFTL
                     PLPSDSQQSSIDYVPSPEIALRINAPKKLQPAIAIPEDASASLLPSHTYKIRAAEKVR
                     TFKFSSFVWVGKRNNLLGGSDYFTKELKIYPGENFDISTIQKTTMRTEND"
BASE COUNT          484 a          298 c          350 g          492 t
ORIGIN      
        1 gcaaaaaata tcttaactat ctgataatga tgggttttaa catatacggt attgttaagc
       61 aggccccaaa gattggggcc tagaagtgtt attgaacagg gtgatgggcc ggttcagaca
      121 gaaaaggtct tagatctata tgaaccgctg ctgcattact gtcattggtc acgtttaacg
      181 gggccttacc cggtaaagac atagtggcaa cgtctggacc agaaacagta aaattatttt
      241 ctgcatttgg cattactgtg aaaattttaa tagctacatt ctcatgacga tacattgaaa
      301 ttccgaaagt tgatccagct tcagtattgc tgacatcaac ttcaaccggt gttttcccga
      361 tcacttcatt agtaagtata tttttcacaa ttgcaccttc tggggcagtg taaatgcgat
      421 aagggtaagc acttgcagtc tggcagaaaa aaatacacaa aacggtcaat atatatttca
      481 tgattgctcc ttttgcaaaa attctaaatc aaatgattga gtgactacaa tttgtttgga
      541 atcaaatgaa cctgggaatt tatatttaat actgaacagt aatggcaatg tgagtccatc
      601 tcgtgaagac tgtgagatgt tcttaaatga gttattaata aatgttaatt ggtcattgtt
      661 atcgtaacaa agaccaagta aaccaactgt gggcaccgta aaaattgtaa ctgaactagg
      721 tgatatcaac tgttttccac gtgacgaata ggtgcaacga ctcccgccga gattcagatc
      781 agacaactca ataaattctt tggtcccatt cttggcgagt aaccagacat cagtgcctga
      841 actctcaatc agaaaagtaa cttttccgtc ggtgtaggct tcagttctgt tgctcattat
      901 agggtcgaac cctaccgaag ggaggactgc aattttgctt ccaaccgact gacatccagt
      961 cacaagtaag gtaagaataa atgctgaaac tctaatcatt ttcagtcctc atagtcgttt
     1021 tctgtatggt actgatatcg aagttttctc ctgggtaaat tttcaactct ttggtgaaat
     1081 aatctgaacc accgaggagg ttgttacgct ttccaaccca aacgaacgac gaaaatttaa
     1141 aagtccttac tttttctgcc gctctgatct tgtaggtatg tgaaggtaat aaagatgcac
     1201 tagcgtcctc cgggattgcg atagcgggtt gaagtttttt gggggcattg atacgtaatg
     1261 cgatttctgg agaggggaca tagtcgattg acgattgttg tgaatctgac ggtaggggca
     1321 gggtgaattt tttaccctga tctgggccaa aaatggcaat aacctctttt ttctgctttg
     1381 ttacggacac taacgcctgt aacggggtac tggatagatc attgacgaat gaaattgtca
     1441 tgctggaacc aggttctaac cagagaattc gtttcccgga tgcatcttca tcaagggaga
     1501 taaattttga tataaacacg ccattttttg caatgatgga aaattcaatt ttgggtatta
     1561 caagatttaa aggagttgaa gcagagtcgt tttcaatctg aagatagtta cgcttagtca
     1621 tgct
//
LOCUS       DAFNBJ010000336         1620 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.345, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000336 DAFNBJ010000000
VERSION     DAFNBJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.345"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(3..1028)
                     /locus_tag="L2X49_004982"
     CDS             complement(3..1028)
                     /locus_tag="L2X49_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HBN4799910.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            complement(1054..>1620)
                     /gene="rfaY"
                     /locus_tag="L2X49_004983"
     CDS             complement(1054..>1620)
                     /gene="rfaY"
                     /locus_tag="L2X49_004983"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBN4799911.1"
                     /translation="DDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLI
                     VETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEI
                     KASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIA
                     NEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          506 a          332 c          272 g          510 t
ORIGIN      
        1 tattatccaa atagttttct tttatactta tttaatttaa atttaatgaa gtaaataaaa
       61 gcgttaaacg tatcaccatt tttctttgca tgttgatatt tcacttgata ctgcttttca
      121 ttcgtggctg gaataaacgc cacatcattc cagcaagaag cctgataagc ctcattaaaa
      181 tacttctggc agggataatt cgtccagtcg ttccagggct tcgttacgcc aacataatga
      241 atcagcttag tatcatcggt aatggtcttt ttataatctt catcgctttt attttttaac
      301 tcatagtcaa tgccataaaa gcaatcataa tcgccgctaa taaaaataca atgaccaaag
      361 aacagaatat ttaaaatatc ctgatcaagg catttatact tcgtgtgctg cgggtcactt
      421 gccagcatag caatggcttt attcataaaa tcatttttcg cccatgcttc aagctgcaga
      481 tatactacac cggcgttgaa ataattccct ggaagtccct ctatagccag acgcttcgct
      541 ggtttttcct gcataaaagg cacatcttta ataacagctg cgaacttatc ctcatcaaaa
      601 ttaatatcaa gcaatccagc taatgagcct ttacagatga catcagcatc gatatataac
      661 agattttcaa tagtttcact caaatactgg aacgcaacta aacggaagaa tgttgcataa
      721 gaccactgtt tcaggctggg gaagatatca ataaaggcat tggagacttt ataaattatt
      781 acttcaacgt tagcattttt gctggtaaaa gcattaacac gttgtagata atcttcatca
      841 atgtagtcgg taaagagatg aaacttaacc gcaaggtccg ggttatgcat aacaactgat
      901 tgcatagata ctgccgcacc aaataaaaag ttacggtcaa taccgtaagc gatatttaac
      961 acacgatcgg tgttagcaat ttccccagag acagcgactt cagtaatact ctcagctaat
     1021 aaatccatgt tggttccgtt ttgactgtgt ggtttacgct ttgcctttta attttttaat
     1081 aaatttgcgt agtttggttc tgtagattac tgaatagtag ccataatctt ttatttcgtt
     1141 ggcaataccc aggtgacgtt ccatggccag gcgatcacga gctttacgct ctgccgtaca
     1201 gcttttaccg gataggtcaa ttatcctgac accatcttta ctaacgataa agttaccccg
     1261 gtgtgggtcg cccgatagca tatttaaggc atgcaatttt tccattgaag ccttaatttc
     1321 tgctttaaca ttttctggaa taatcggcat atcattgagc tctacgccct cgacatactc
     1381 gataagcatg ataaaaacac tggcatagtt gaaaatttta cgctcagcca gaaaataaaa
     1441 atcattagga aaagtaagac ctgcacttct gacacgatcg gtttcaacga tcaaattctg
     1501 atagtagtca ccttttacaa aagattttaa aaaacgttca ttccttttcg ttttcggagc
     1561 aaagacttta aaaacatagc gaccatattt cgtatcaatc aacgatactt tggtatcatc
//
LOCUS       DAFNBJ010000337         1607 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.346, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000337 DAFNBJ010000000
VERSION     DAFNBJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.346"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(16..1491)
                     /gene="msr(E)"
                     /locus_tag="L2X49_004984"
     CDS             complement(16..1491)
                     /gene="msr(E)"
                     /locus_tag="L2X49_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(E)"
                     /protein_id="HBN4799912.1"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS
                     SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG
                     GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD
                     MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ
                     QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE
                     HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG
                     KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL
                     ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM
                     MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
BASE COUNT          436 a          356 c          280 g          535 t
ORIGIN      
        1 ttagattggc tatcattaac aatctctctc aaaagtcttg atgattttgt ggtctttgat
       61 ctcgtagata atgtcagcaa tattatcgac caattgcttg tcatgagata cgaagataat
      121 agttcctgca taggacttca tcattgtttc taatgcggca atacttttta ggtcaagata
      181 gtttcctggt tcatccataa gcaaaatatt atattttcct aaaagcattt tggataaaag
      241 cagtttgatg atttcacctc ccgataagtc ggataagttt ttttgaatat cattcgctcc
      301 gatccccatt gaagccaata ctgcacgaat ttccgcaact gtgtactcgc actcttcctg
      361 cataaaggag agcacagatt tatgcgtgtt aaatttatat cctgtttgtg taaagtagcc
      421 aatttcagct tttggagata tggttaatcc atcagcacgt tctgatatca tttttaacaa
      481 ggacgttttc cctgttccat tcgatccagt tatagcgact ttagcgccaa gcggtattat
      541 aaagttagcg tcatcaaaga tagtacggct accaaatttt aagctcagac catctgccgt
      601 aatcgggaac ttattgtgca gttctagggc tgaactttga cgaaaacgaa tagaacgcaa
      661 atgctctggt gcttgaatat cttctaatgc agccaaacgc ttttccatac tcttagctgc
      721 ctgatacagt tttctttgct tggtgccagt catttttgca tgcccaagtc gtccagcact
      781 ttcggtagag tttttggatt tttctccttt tttcttattg tctaatcgat tagcttgctg
      841 gcgtttttct tgcacagcag attctaatcg ctcccgttcc ttcatcatca gctcatattc
      901 tacggcttgg tgttgtcgct cttcttcttt ttgacgcaag taatccgagt aaccacccca
      961 atattccgta attttaccgt cttttaactc ccatatcttg tctacaacca tatcaagaaa
     1021 atatcggtca tgactgataa caagtaatgc tccatcaaat gctttaagtt gaccaataag
     1081 tagatctatt ccattgagat caaggtggct ggttggttca tccgctagaa tgccatgtac
     1141 ttgttgggaa aatgcggcag caatttttgc acgagtttcc tctccgccac tcattgtgtc
     1201 gttttgtaca ttggaaacac caaggcgaga taacattgcc cggtcttcga ccgtttctat
     1261 ttcgattccg cccagttggc tgatatgtgc aaaatcacca aaacgctgta atgtcgcttc
     1321 ggctaaaaca atttcgccat taagtacttt gagtaaacta ctctttcctg ctccgttatc
     1381 acccacaaga ccaatacggt cataagagtg aatttccaat tcatcaatat ccaaaacatc
     1441 acgcccagca taatccaagc gtatgtttct cgctttaata attaaactca tttttattta
     1501 ctcctgttta gctcttgaaa ttttttatgc agcaaacagg atttaggtga aaacaaaagc
     1561 tagcatcaca gtgtcctccc aaaaaaaaag ctattcatcc acagggt
//
LOCUS       DAFNBJ010000338         1591 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.347, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000338 DAFNBJ010000000
VERSION     DAFNBJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.347"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>1591
                     /gene="rhsB"
                     /locus_tag="L2X49_004985"
     CDS             <1..>1591
                     /gene="rhsB"
                     /locus_tag="L2X49_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HBN4799913.1"
                     /translation="RGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEICYRYD
                     SDGRVTEQLNPAGLSYTYQYEKDHITITDSLNRREVLHTQGEAGLKRVVKKEHADGSV
                     TQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLTTPDGRASAFYYNHHNQLTSATGPDG
                     LELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLL
                     SFTDCSGYQTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNI
                     AGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTF
                     RYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGET
                     AERWRYDERGWLTDISHISEGHRVAVHYRYDEKGRLTGERQTVHHPQTEALLWQHETR
                     HAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGMKLGDTPLVDFTRDRLHRETLRSFGR
                     YELTTAYTPAGQLQRQHLNSLQ"
BASE COUNT          384 a          478 c          490 g          239 t
ORIGIN      
        1 cgcggcgaac tggcggcggt gtatgaccgc agcaatacac aggtgcgcag ctttacttac
       61 gatgataaat accggggccg gatggtggcg caccgtcaca cgggccgacc ggaaatctgt
      121 taccgttacg acagcgacgg gcgggtgaca gaacagctaa acccggcagg cttaagttac
      181 acgtatcagt atgagaaaga ccacatcacc atcaccgaca gcctgaaccg ccgtgaagtg
      241 ctgcacacgc agggcgaagc cgggctgaag cgggtggtga aaaaggaaca cgcggacggc
      301 agcgtcacgc agagtcagtt tgacgcggtg ggcaggctta aggcacagac ggatgccgca
      361 ggccggacaa cagaatacag cccggatgtg gtgacgggcc tcaccacgcc ggatggcagg
      421 gcatcggcgt tttactataa ccaccacaac cagttaacgt cagccaccgg gcctgacggg
      481 ctggaattgc gccgggaata tgatgaatcc ggccgtctga ttcaggaaac cgcccctgac
      541 ggcgatatca cccgctaccg ttatgataat ccacacagtg acctcccctg cgcaacggac
      601 gatgccaccg gcagccggaa aaccatgacg tggagccgtt acggccagtt gctgagcttc
      661 accgactgtt ccggctacca gacccgttat gaccatgacc gcttcgggca gatgacggcg
      721 gtgcaccgcg aggaagggct gagtcagtac cgcgcatacg acagccgtgg acagttaatt
      781 gccgtgaaag acacgcaggg ccatgaaacg cggtatgaat acaacatcgc cggtgacctg
      841 accgccgtca ttgccccgga cggcagcaga aacgggacac agtacgatgc gtggggaaag
      901 gccgtccgta ccacgcaggg cgggctgacg cgcagtatgg aatacgatgc tgccggacgg
      961 gtcatccgcc tgaccagtga aaacggcagc cacaccacct tccgttacga tgtacttgac
     1021 cggctgatac aggaaaccgg ctttgacggc cgcacacagc gttatcacca cgacctgacc
     1081 ggcaaactta tccgcagcga ggatgagggg ctggtcaccc actggcacta tgacgaagca
     1141 gaccgcctca cgcaccgcac cgtgaagggt gaaaccgcag agcggtggcg gtatgacgaa
     1201 cgtggctggc tgacagacat cagccatatc agcgaagggc accgggtagc ggtgcattac
     1261 aggtatgatg agaaaggccg cctgaccggt gagcgccaga cggtgcatca cccgcagacg
     1321 gaagcactgc tctggcagca tgagaccaga catgcgtaca acgcgcaggg actggcgaac
     1381 cgctgtatac cggacagcct gcccgccgtg gaatggctga cctacggcag cggctggctg
     1441 gcaggcatga agctcggcga cacaccgctg gtggatttca cccgcgaccg cctgcaccgg
     1501 gaaacgctgc gcagcttcgg tcgttatgaa ctcaccaccg cttacacccc tgccgggcag
     1561 ttacagcgtc agcacctgaa cagcctgcag t
//
LOCUS       DAFNBJ010000339         1587 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.348, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000339 DAFNBJ010000000
VERSION     DAFNBJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.348"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(161..814)
                     /gene="ribB"
                     /locus_tag="L2X49_004986"
     CDS             complement(161..814)
                     /gene="ribB"
                     /locus_tag="L2X49_004986"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBN4799914.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(918..1066)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1188..1478
                     /gene="ubiK"
                     /locus_tag="L2X49_004987"
     CDS             1188..1478
                     /gene="ubiK"
                     /locus_tag="L2X49_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBN4799915.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT          457 a          407 c          353 g          370 t
ORIGIN      
        1 atatgatttt gatcataatg aaaaagaatg cctgatatta atgaaatttt ctcatagaaa
       61 gagaagcgga attaacatct tgcatagcag ggaaaaataa aaaccgaagc ctgctacaac
      121 ttcggtttct tgattaaggc agtaaattaa gcagcggttt tcagctggct ttacgctcat
      181 gtgcctgacg gtatgccacc aagtcttcaa tagtcacgag cgccatgttg tgtttattgg
      241 caaactcaat gcactctggt gcacgcgcca tcgtgccatc gtcattagtc agctcacaca
      301 gcacaccagc cggtttaaag cctgccagcg tcatcaaatc aatagtcgct tcagtatgac
      361 cgccacgcgt cagtacacca cctgcctgcg cgcgaagtgg gaaaacgtgg cccgggcgat
      421 tcagatcaga aggttttgcg ccatcggcaa tcgctgcgcg aacggtcgta atacgatcag
      481 cggcagaaac accggtagtc acaccttcag ccgcttcaat ggtcacggta aaaccggtgc
      541 cataggcgct ggtgttattt tctaccatca ttggcagatc aagttgttta cggcgatctt
      601 cagtgatgca caggcaaaca ataccgctac cgtggcgaat ggtcagcgcc atctgctcaa
      661 cggtcatggt ttctgccggg aagatcatat cgccttcgtt ttcacggtct tcatcatcaa
      721 gcaccattac accgcgtcct tcacgcagcg cagccagtgc attttcaaca cgttcgaaag
      781 gcgtaccaaa agaggaaagt agcgtctgat tcatggtaaa aaaacctcac taaaattatg
      841 gttaccagaa tcagggcagt cttaggagtg gcggcatata gccaaaataa cgtgagcggg
      901 tccatgcccg acagaatcgt tactctctcc catccggact ctaaccgtcg gccccggaat
      961 tacaccggat ctgctgtcct ttgagttcgc actcaaagcg ctcgcgggct ttcaaccaag
     1021 ttgatttacc gccggtgggg aatttcgccc cgccctgaga ataagcggat tcactataac
     1081 gctaatgatt agcggcagca acgcatagct tcacataatt ctggtttatg acttaccctt
     1141 atcgcactac aatggcactc aacacctatc agtacaggga aactacaatg attgacccga
     1201 aaaaaattga gcaaatcgct cgccaggttc acgaatcaat gcctaaagga atcagggagt
     1261 tcggggaaga tgtggagaaa aaaatccgcc aaaccctaca agcgcagctg acgcgcctcg
     1321 accttgtaag ccgtgaagag ttcgacgtcc aaacgcaagt cctgttacgg actcgtgaga
     1381 aactggcgct gcttgaacaa agaatcagcg aactggaaaa tcgcagtact gagatcaaaa
     1441 agcagccaga tccagagact ctccccccaa cgctgtaaac atttgtcttt aatgcactgt
     1501 aaattatgaa ttaatcctct aatttacagt gctttctatt accttatcgc gtaaccccat
     1561 tcagaaaagc atcacaaaaa tgaatga
//
LOCUS       DAFNBJ010000340         1554 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.349, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000340 DAFNBJ010000000
VERSION     DAFNBJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.349"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..652)
                     /locus_tag="L2X49_004988"
     CDS             complement(<1..652)
                     /locus_tag="L2X49_004988"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBN4799916.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTM"
     gene            complement(816..911)
                     /locus_tag="L2X49_004989"
     CDS             complement(816..911)
                     /locus_tag="L2X49_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799917.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1025..1192)
                     /locus_tag="L2X49_004990"
     CDS             complement(1025..1192)
                     /locus_tag="L2X49_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799918.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1195..1359)
                     /locus_tag="L2X49_004991"
     CDS             complement(1195..1359)
                     /locus_tag="L2X49_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBN4799919.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT          424 a          371 c          355 g          404 t
ORIGIN      
        1 tcatagtggt gtgcgagtca gtaccaacga gtgtatccgg ataagcaatc cattcaccgt
       61 cctgcaattc actccacact gctttgccga gatattcgag gttaacctga tggcaaatgc
      121 ctgtgcctgg cggcacgacg ctaaaccgac tgaacgcttg ctttccccat ttcaggaaca
      181 cataacgttc gtggttgcgc tccatttcca ggcgtacgtt ttcttcaaat gcctcatcat
      241 caccaaaacg atcgacggtc accgagtggt caatgaccag gtcgaccggt gagagcgggt
      301 taacctttgc agtatcgccg ccgaggcgtt taaccgcttc gcgcattgcc gccagatcaa
      361 caacggcagg tacgccggta aagtcctgca tcagcaccct tgccgggcgg taggcaattt
      421 cacggtcagc atgggcattt ttcagccatc ctgccagcgc gtggatatcc tcttcggtaa
      481 ccgagttacc atcctgccag cgcagcaggt tttcgagcaa aactttgagt gacttgggca
      541 gacgggtgat atcgcccagt gatttagcag caagcggcag gctgtagtag tggtaagttt
      601 tatctttggc ctgcaacgtg tccttactgg cttctcgtag ggttgacgac atagctcctc
      661 cttaatgaca gggttgcgaa gccctgattc tcttcagggt gaatattaaa gataacacaa
      721 acatcgcgta acgatttgat aacaacccaa attgataaaa gaggagaaac cttgtggcaa
      781 agcaaatgac aaccccgccg cagcggggtc aaggatcagt gagtaatgat ccagaggagt
      841 tcggcccaga agagcatcga cagagaaaac gcgcccatcc aggaccaata tttcatattt
      901 gtgttgatca taggctacac ccttaacggt tattattttg tgttgagata actcaagctg
      961 ttattgctgc ggtgtattaa aagcccttta attcattaaa aatgggtaat aagaatgatg
     1021 ataattattg tttcggctga tcatcttcag caaacagttc tataaaggct tgctgctgcg
     1081 atgttagttg ccagtcgtct ggaataacga cggaacattc ccgtttcgtt tctgtttcat
     1141 tgtcactatg ctggcataat ggtgacgact ccagttgctc ctgaccgacc atagttatcc
     1201 ccacactttc caaatcagca gtgcgacaac cacccagaaa agtgcagaac caagaaaaac
     1261 cgccagccag gctttacgtt ttaattcagg atcgcggcgg ggttcctgat ttccagaagg
     1321 cattgctaac ctcatataat cgccatcact tatcagcaag acgttgaacg attggtgtaa
     1381 ttaatcatac gatgagacaa atcctaaaga aactttagtc aaaaatccag cgaatttacg
     1441 aatcgtctcc agtgaataat tcaatctttt gacctgaaat agatatttga aaactggagt
     1501 ttgcgaggcg ggtaattaga cactacttta caggtaattt accctataaa tatg
//
LOCUS       DAFNBJ010000341         1537 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.350, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000341 DAFNBJ010000000
VERSION     DAFNBJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.350"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            27..>1537
                     /locus_tag="L2X49_004992"
     CDS             27..>1537
                     /locus_tag="L2X49_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN4799920.1"
                     /translation="MDTAEHDGKFAWASFYEAFANALLTWRNRRDELVKGIHLIASGV
                     EGMSHLQDKSIMGEIFPLKDICPFTTMGLFNRNLTDSNRKIIAAKLANLLGVNEPIPD
                     SFAGIPLLNNQKSWFFGYEKSRDPNDIECLWEMFSQAMTFADNQNTNSADFTAAYDIA
                     STVMNVGWNLTMGLYWTRPWFYPTLDSQSQYYIQTVLNIKIIKNGAKGRCSGQSYLSL
                     MRALNEVFTQPNYPVHSFPELSLSAWNTDLSQSKDEVANRTWKASLLNKIKALCLKKN
                     GPYFTASELEENYLDVFKVEHPESKTVTSSIYFYLQKLCNDDELKSLESGNYEYLNFE
                     KNESQTITEETVEESAPLPKLTHVPYDISHLVQDGCFLEEAKIQLTLQRLIDKKNLIL
                     QGPPGTGKTWLARRLAYCLMGEKAPERISAVQFHPNLSYEDFIRGWRPGKEGQLTLID
                     GPFVNAIKTAVNNPTSKYVVIIEEINRGNPAQIFGETLTLMEADKRTPTEALSL"
BASE COUNT          455 a          326 c          347 g          409 t
ORIGIN      
        1 tgctcaacaa gtataatgga gttcccgtgg atacagcaga acatgatggc aaatttgctt
       61 gggctagttt ttatgaggct tttgccaacg cgttactgac ttggcgaaat cgtagagatg
      121 aattagtcaa aggtattcat ctaattgcct caggtgtgga aggaatgtcc catctgcagg
      181 acaaatctat tatgggagag atctttccct taaaagacat ttgtccgttt actactatgg
      241 gcttgttcaa tcgtaacttg accgacagca atcgcaaaat tattgctgcc aagctagcga
      301 atttgttggg cgtgaacgaa ccgattcctg actcctttgc cggaatcccg ctgttgaata
      361 accaaaaatc ttggttcttt ggttatgaaa agtcacgcga ccctaatgac attgaatgct
      421 tgtgggaaat gttctcgcag gcaatgacct ttgcggataa ccagaatact aactctgcag
      481 acttcactgc cgcatatgac attgcttcta cagtaatgaa tgtcggttgg aatctcacca
      541 tggggttgta ctggacccgt ccttggttct accctacttt agatagccag tcgcagtatt
      601 acattcagac agtgttaaac attaagatca ttaaaaacgg ggcaaaaggt cgctgtagtg
      661 gacaaagtta cctgagtctt atgcgggcgc tgaacgaggt ctttacccaa ccaaactatc
      721 cggttcattc tttcccagaa ctttctttat cagcgtggaa tacagatctt agccagtcta
      781 aagatgaggt ggcaaataga acgtggaaag cctctttact gaataaaatt aaggcgttat
      841 gcctgaaaaa aaatggccct tatttcactg cttcggagtt ggaagaaaac tacttggacg
      901 tattcaaggt agagcatcct gagagcaaaa cagttacaag ctcaatctac ttttatctac
      961 agaaactatg caatgatgat gagttaaagt cccttgagtc cggaaattat gaatacctga
     1021 attttgagaa aaatgaatcc cagacgataa ctgaggaaac tgtcgaagag agtgcgccgt
     1081 tgccaaaact cactcacgtt ccttatgaca tcagtcatct ggtgcaggat ggctgtttct
     1141 tggaagaggc taagatccaa ctcacacttc agcgcctgat cgacaagaaa aacctgatat
     1201 tacaaggacc acccggaact ggaaaaacct ggttagcgcg acgtctggcg tactgcttga
     1261 tgggagaaaa agcacctgag cgtattagcg cggtgcaatt tcacccgaac ctctcttacg
     1321 aagactttat tcgcggctgg cgtccaggta aagaagggca attgacgcta attgatggac
     1381 cttttgtaaa tgcaatcaaa actgcagtca ataatcctac ctcgaaatat gtggtgataa
     1441 ttgaagagat taatcgtggc aatccggcgc aaatcttcgg tgaaacactc acgttgatgg
     1501 aagcagataa acgcacgccg actgaggcat tatctct
//
LOCUS       DAFNBJ010000342         1497 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.351, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000342 DAFNBJ010000000
VERSION     DAFNBJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.351"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(14..1066)
                     /locus_tag="L2X49_004993"
     CDS             complement(14..1066)
                     /locus_tag="L2X49_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN4799921.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERYTMG
                     EIEAKPWLGESVDRRTLKDMVELWFKLHGKSLTAGQHVYNKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAVNLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDCYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TLPSGDKVAAAVGITP"
     gene            complement(1158..>1497)
                     /locus_tag="L2X49_004994"
     CDS             complement(1158..>1497)
                     /locus_tag="L2X49_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799922.1"
                     /translation="TNLQLIYIFTRGLVGIAILSWLSYICLSNSKKYTHESIRRKDRR
                     HALMFGQVFLQIYGSTATKEDAIEVFKDWNISGDSAFSGQTEQPPSFASFLNTIKDKV
                     KVTGSDKETD"
BASE COUNT          371 a          337 c          335 g          454 t
ORIGIN      
        1 tcagtggtgg ttattacggg gtaatgccaa ccgctgccgc cactttgtcg ccacttggca
       61 gcgttgccag aggattgaaa cggagcgccg tttccagatg atccggtgcc agatgtgcgt
      121 aacgcatagt catttttata tcgtggtgtc cgagaatttt ttgtaaggcc agaatgtttc
      181 cacccgacat catgaagtgc gctgcaaacg tatggcgcag aacgtgtgtg agttgaccgc
      241 gagggagcac gatagacgtt ttttccatca cggataaaaa ttgaaaatag cagtctgtga
      301 agaaattgaa cccatcgagc gccatgatct cttcgtaaag ctctttactg atagggatgc
      361 ttctgttttt cttccccttc gttctgacaa aggtaattcg gtatttggtc acctgtgagc
      421 gggtaagatt tacggcttca cgccagcgtg cgcctgtgct taagcatatc ttaactacca
      481 gtgccagaat tgggtcctga cgtttgcaat cagccagcaa ttcaacaatc tgctcatggg
      541 taagccatgc catctctttt tctgcgatgg tgaattttcg catgttctcc agtgggttcg
      601 gatacgacca ttcgcccaga cgggatagtt cgctaaaaac actacttaga tagctttgct
      661 ccaggttaat ggtgaccggg cttgctcctt tcttccattt ctcgctgaag tagatctcgc
      721 ctgtcaggcg tttatctcga tagtgagcaa acatttttga ggtgagatca gttgcaaggg
      781 gatttcccaa ggcgtcaacc atcaacagca gcttgttgta gacatgctgt ccggcagtaa
      841 gagatttgcc atgtaatttg aaccatagct caaccatatc tttcagtgtc cgacgatcca
      901 ctgattcgcc cagccagggt tttgcttcta tttcccccat ggtgtatcgc tcgaaggcca
      961 gtgcttcgcc tttggtggcg aattgtttac gcacacggcg tccgctacgt ccggcggggt
     1021 aacattcgca aagccatttt cctgtggtga gttttcgtac tgccataaaa aatgccctca
     1081 tatcagaggg ctaaatttaa ctgtatataa caacagtatt caatgtattt tttacttagt
     1141 agcatacatg ttcatgatta atctgtttct ttatcacttc cagttacttt aactttgtct
     1201 ttgattgtat tcaaaaatga cgcaaaactc ggtggttgct ctgtctgacc tgaaaatgca
     1261 gagtcacctg aaatattcca atccttaaag acttctattg catcctcttt agttgctgta
     1321 gaaccgtata tctgcaaaaa aacttgacca aacatcaaag catgtcgacg atcttttcgc
     1381 ctgatcgatt catgtgtgta cttttttgag ttactaaggc agatatatga tagccatgaa
     1441 agaatggcga ttccaactaa tcctcgcgtg aagatatata ttaactgaag gttagtg
//
LOCUS       DAFNBJ010000343         1483 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.352, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000343 DAFNBJ010000000
VERSION     DAFNBJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.352"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            9..641
                     /locus_tag="L2X49_004995"
                     /pseudo
     CDS             9..641
                     /locus_tag="L2X49_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(686..1162)
                     /locus_tag="L2X49_004996"
     CDS             complement(686..1162)
                     /locus_tag="L2X49_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBN4799923.1"
                     /translation="MRSPKVKFLTIFTFCIFITKMSFASNSCSNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKNNNIKSLDYWLMQINQMHIPLLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFAMDNQKKRQQYAPKYILYIPGLMN"
     gene            complement(1245..1463)
                     /locus_tag="L2X49_004997"
     CDS             complement(1245..1463)
                     /locus_tag="L2X49_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799924.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
BASE COUNT          464 a          225 c          282 g          512 t
ORIGIN      
        1 atgaatatat gggaaaaatt aaaattgtag tttcagatca gcagccgttt atgattgatg
       61 ggataattgg atttctcgga cattatcctg atttatatga ggttgttggg ggctataaag
      121 atctgaagaa atctatagcg gagtgtaaca aatctacagc ctaaatattt attttaggag
      181 aatttgcagg tggaatgatg gggtccgaac tcgtaaaatg ggttaaatcg cataagatag
      241 atgctcatat tattacattt gtagcaaaga tgccgtatat tgattcaata aaattgcttg
      301 aagcaggtgc gaaaggatgc gtatggaaaa ccagtcaccc ggcgaaacta aatcgtgcta
      361 ttgatgcgat tagtaatggt tacacttatt ttgatagtgt acatatggat tgtgaaaaaa
      421 tatcttccag gtattcctct gataatcaac ttacaaatcg tgagtcagaa atattacaac
      481 tcatagctga tggtaaaaca aacaaagaaa tcgctaattt cttgcagtta agcagaaaaa
      541 cagtcgaaac tcatagactc aatatcatga agaaattaga tgttcacagt ggtatcgagt
      601 tgatcaaaac agctttacgt atgggtgttt gcactatata aaaaaaagag gctttaggct
      661 cattttgctg aagccttacg attgtctagt tcattaagcc tggtatatac aatatatatt
      721 ttggggcata ttgctgtctc tttttttggt tatccattgc aaaacccgca ttataagtcc
      781 ccaggcattg ccacgttact ccacaacgtg aaaaatggtt atataaaatc tcggttccta
      841 ttttaatgtt cagacaaggg ttatctaaca aatctcgttc atcttttatt attccacgtt
      901 tttttaacag gggaatatgc atctggttta tctgcattaa ccaataatct aaactcttta
      961 tgttgttatt cttatctctg ttcttgccta tgctatcctt ttttagattg ctttcaacaa
     1021 gagcgatagc tttaattaaa tttggttcga ttctgaacat tgttcctgct tcattagagc
     1081 aagagttgct ggcgaaagac atttttgtga taaatataca aaacgtgaag atggttaaga
     1141 atttaacttt tggtgagcgc atattttttc atgtgtgagg attgaatgaa aggaacgaga
     1201 tactattata aaagtatctc gttatgattt atttggtcaa tcgtctacca ttcaatttta
     1261 tagtaaggtg agataggatt atcttctgaa tacatatcat tttgaaaagg cgccatccat
     1321 gttgaaacta taaatgcttg tatttgatct gtatcagtag cactggtgtt gatgtgttta
     1381 tctaatgata cgacttcatc acccgttgca ttcgctatat ttgaactttg agataaatta
     1441 ttgataccga ttggattagt cataaagctc ccttttttaa ttg
//
LOCUS       DAFNBJ010000344         1459 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.353, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000344 DAFNBJ010000000
VERSION     DAFNBJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.353"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(107..547)
                     /locus_tag="L2X49_004998"
     CDS             complement(107..547)
                     /locus_tag="L2X49_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799925.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDAL
                     RILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGV
                     IFEGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
BASE COUNT          311 a          385 c          358 g          405 t
ORIGIN      
        1 aaaaacacgc aagggatttt gaggcggcaa aagagcaatg caggatagac aatccactag
       61 aaattgcatg caatgttctg tggattgtcc gcctgccgct ggctctttaa atccggccgc
      121 cctgaatgac actaaagccg tttttgagtg cctttttgtg gttggttacg gtgcctttcc
      181 aaccaaattg ttcattcagc cggtatgttt ttgatctgcc gactttaggt ccctcaaaga
      241 tcaccccaac atccagtagc gcgcgaacgg cgcgactaac attcgttttc tgcatcccta
      301 aagtttcagc aatatctgct tgggcgatct gaatgaagtt ttcgtaatcc agatctgcaa
      361 gcatcagcat taagaccttg aattgctccc cggttaattc cttagccaag atccgtaatg
      421 cgtcttgatt catggtgaaa tgcctctgaa atgctgattt ttgtttaggg cggataacgg
      481 caacgaagcc gccgataatc tcaccctggt catttatctg agtgattctc tcgacatcct
      541 tactcatctt tgaaccctat ttgagttatc aataatgata acagtgttat cattattgat
      601 aactatgtca actgtaaccc ttagatttta ttaatgtttt tattctcctt tctgtatctt
      661 tatattaggc gtctaacgca ttgattccat tagatattta tcctagctgc tctttggggt
      721 gtcggggcga agccctgacc cagtcacgta gcgatagcgg agtgtatact ggcttactcc
      781 tctgcaagcc tcgtcagcca tgatttttgt ccgatttctc gctctccacg ttcaacagct
      841 ccatcatgtt tttgccgtcc gttcaccgtt aaaacgcctc agaaatcagg acaggatgtg
      901 caaatgtaat accgtcaccc gcgacgatat tccgtttgcc atctggtcag agcttcgccc
      961 cgacaccccg tatcagtgcg tacgtgctca atgtggtttc ctgttctctt ccgcttcctc
     1021 gcccactcgc tcactaacgt tcggtcgctg gggctgcggc gagcggtgcc acctctcgca
     1081 tgctgtcggt gattccgctg ttgttctttc ggggatgagt cgtcagatag cccttcaaac
     1141 gcgctcagaa gctctcagac gcacgaggcg gggtttaaat gcccgtcagg gcatggaagg
     1201 cgcgaaaaac gcttacagag cgtttcagga ggtcaggcgc gccgataccc ctagccaggg
     1261 gctttcaggt cgccaggaac cgcgccagcc ccctgcaaag cccatttgca cggtacaagc
     1321 agccactcgg ttggtaggtg gtcgtagacc gtcggtgtta cagcgatggg gggtctgggg
     1381 gtctccccag acggtttccg gttgcgctca aggcgctgcc ggaaccgtgg cggctcggtc
     1441 gttctggaga tcggggagc
//
LOCUS       DAFNBJ010000345         1450 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.354, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000345 DAFNBJ010000000
VERSION     DAFNBJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.354"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..224)
                     /locus_tag="L2X49_004999"
     CDS             complement(<1..224)
                     /locus_tag="L2X49_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBN4799926.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNI"
     gene            complement(236..>1450)
                     /locus_tag="L2X49_005000"
     CDS             complement(236..>1450)
                     /locus_tag="L2X49_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBN4799927.1"
                     /translation="WRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQ
                     PGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEIL
                     ADRVSEESRRWLASCGLTVEQMKNQMDPVYTPARKIHLYHCDHRGLPLALISTEGATA
                     WCAEYDEWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPI
                     GLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPT
                     SIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNTGKGQNSFASQLAKELHTNVI
                     GPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISN
                     MQGTPEKPSDIR"
BASE COUNT          336 a          385 c          314 g          415 t
ORIGIN      
        1 tcaatattat ttttagtaaa gcagtaatat tgatatagtc gaaatgatgc ctctgaatca
       61 ccatgttgtg atttatactt cagtttcatc agcttttctt cacttaaatc ataggtcatt
      121 gctgtagatc ccatcacact ttcgttttct tttattttca catcagcacc atgcaattga
      181 ccaataagag aaaaataaaa aaatagtaac aagcatgctt tcatacatct acaacctatc
      241 ttatatcact gggtttttct ggcgtacctt gcatattaga aatggggtgc ccagatggcc
      301 catatgttat ccaattacca aggtcttttg ttgttatagc cattagatcc ttatttgaat
      361 taaggttcgt tggtgctgtt agcactgtat ccatttttaa cttaccatta gttcctcgcc
      421 cccaccacgt ccatagcgtg tcaggtccta ttacatttgt atgtaactct ttagctaatt
      481 ggctagcaaa tgaattttgt cctttacctg tattacaaga aaataaccaa actgtcatcc
      541 catctttata attcccatca aatttaatta gatatgctag atctttagct gtcatgatac
      601 tgcgcgttgc ggattcaata gatgtggggg taccatgccc cccgattgtg aaaacgcctg
      661 ggatattaat ctcatcagct acagaatgga taagatcact ttcggggtat aaatttatat
      721 caaccaaacc ttgcggatct acatttatga ccggattcaa cggatactga taaaaattcc
      781 atcccccctt cagcccaatc ggatcctgag tgatataccg cccctgcagc gggtcataat
      841 agcggtggcg gttgtaatac aggccggact cctcatcata ctgctgaccc ggcaggcgaa
      901 tgagctgctg cagatgatgc gggttctcat cactcagcag gttgccccat tcatcatatt
      961 ctgcgcacca cgctgttgcc ccttccgtgc tgatgagcgc cagcggcagg ccgcgatggt
     1021 cgcagtggta caggtggatt tttcgcgccg gcgtgtacac cgggtccatc tggtttttca
     1081 tctgctccac ggtcaggccg cacgatgcca gccagcggcg gctttcctca ctcacccggt
     1141 cagccaggat ttcactttcc agccggtcga gcatctgcac cagcaccggc gggaacacca
     1201 cactgccacc gtcttcgccg ccggactgct gaagcgcatc cgccaggctg cggcgctgcg
     1261 ttttcgccag ctcaccggtg gcggtttcaa ctctgatgag tggcgtgaag ctccccggct
     1321 gataaatcgt ctggatgcgg gttctgtcgt tctgtattgt ggtcagccgg tcgccgtccc
     1381 agccgtacca ggtcacttgc ggtttccgtg acagcgacat ccagcccgtc aggtcccgtt
     1441 cacgccgcca
//
LOCUS       DAFNBJ010000346         1426 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.355, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000346 DAFNBJ010000000
VERSION     DAFNBJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.355"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            26..1417
                     /gene="narK"
                     /locus_tag="L2X49_005001"
     CDS             26..1417
                     /gene="narK"
                     /locus_tag="L2X49_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBN4799928.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT          252 a          351 c          406 g          417 t
ORIGIN      
        1 atcgaaacga gtatcagagg tgtctatgag tcactcatcc gcccccgaaa gggctactgg
       61 agctgtcatt acagattggc gaccggaaga tcctgcgttc tggcaacaac gcggtcaacg
      121 tattgccagc cgcaacctgt ggatttccgt tccctgtctg ctgctggcgt tttgcgtatg
      181 gatgttgttc agcgctgttg cggtgaactt accgaaagtc ggttttaatt ttacgaccga
      241 tcagctattt atgttgactg cgctgccttc ggtttctggc gcgttattac gtgttccata
      301 ctcctttatg gttcctatct tcggtggtcg tcgctggacg gcgttcagca ccggtattct
      361 gattattcct tgtgtctggc tgggttttgc cgtgcaggat acctccacgc cttatagcgt
      421 cttcatcatc atctctctgc tgtgcggctt tgctggcgcg aacttcgctt ccagtatggc
      481 aaacatcagc ttcttctttc cgaaacagaa gcagggtggc gcgctgggtc tgaatggtgg
      541 tctgggcaac atgggcgtca gcgtcatgca gttggttgct ccgctggtgg tatcactgtc
      601 gattttcgca gtatttggta gccagggcgt caaacagccg gatgggactg agctgtatct
      661 ggcgaatgcg tcctggatat gggtgccgtt ccttgccatc ttcaccattg cggcgtggtt
      721 tggcatgaac gatcttgcta cctcgaaagc ctccatcaag gagcagttgc cggtactcaa
      781 acggggtcat ctgtggatta tgagcctgct gtatctggca accttcggct cctttatcgg
      841 cttctccgcg ggctttgcga tgctgtcaaa aacgcagttc ccggatgttc agattctgca
      901 atacgctttc ttcgggccgt ttattggtgc gctggcgcgt tctgcaggtg gtgcattatc
      961 tgaccgtctg ggcggaactc gtgtcacgct ggtgaacttt attctgatgg cgattttcag
     1021 cggcctgctg ttcctgacct taccgactga cgggcagggc ggaagcttca tggcgttctt
     1081 cgcggtcttc ctggcgctgt tcctgacagc tgggctgggt agtggttcca ctttccagat
     1141 gatttcagtg atcttccgta aactgacaat ggatcgcgtg aaagcagaag gaggttctga
     1201 cgaacgtgcg atgcgtgaag cggcaaccga cacggcggcg gcgctgggtt tcatctctgc
     1261 gattggcgcg attggtggct tctttatccc gaaagcgttt ggtagctcgc tggcattaac
     1321 gggttcgcca gtcggcgcaa tgaaggtatt tttgattttc tatatcgcct gcgtagtgat
     1381 tacctgggcg gtatatggtc ggcattctaa aaaataaatc ttaata
//
LOCUS       DAFNBJ010000347         1387 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.357, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000347 DAFNBJ010000000
VERSION     DAFNBJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.357"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            181..1056
                     /locus_tag="L2X49_005002"
     CDS             181..1056
                     /locus_tag="L2X49_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBN4799929.1"
                     /translation="MSDNNEVTHPFDVTNTETGKTYQLSPNSSKSVQPIALLRLSVFT
                     PVGTKEKRYRNFEVDASDELSSMELARSEGYDDIRITGLKLSMSTDFKCWLGCIMAFS
                     KYGFASDKITLSFNEFAKMCGISSTNINKRTRSRFQEALANLASVVISFRDSKTERFT
                     VTHLVQKAVIDPKKDTVELVGDPSMWELYRYDHKTLLSLQVLSVLAKKEAAQSLYIYF
                     EAMPAGTLFVSMKRLRERLLLTTPVRTQNQIIRKAMLELKSIGYLEYQEVKKGRDIQF
                     QIFKRSPKLALAKHS"
BASE COUNT          409 a          274 c          295 g          409 t
ORIGIN      
        1 attttattat ggaataagca tgatcaggca ttcaggtgca tgtaaaaagc atttgattga
       61 ataagaaaag catttacttg aattgaaaag gcatttagtt taatgcgaga aaagcatttg
      121 cacgaataat ctattcacat ctaaatgtat caagggataa accatggtgg gtaaatttaa
      181 atgagtgata acaatgaggt aacacatcct tttgatgtga ccaacacgga gacagggaag
      241 acttatcaac tgagtccaaa ctcttcgaaa tcggtccaac ccattgcttt actgcgctta
      301 agtgttttta ccccggtggg aactaaagag aagcgataca gaaattttga ggttgacgct
      361 tctgatgagt tgtcgagcat ggagctggcc cgttcagaag gatacgatga cattcggatc
      421 actggtctta agctttcgat gtcaaccgat tttaagtgtt ggctcggttg cataatggcg
      481 ttcagcaaat atggtttcgc ttccgataaa atcacattgt cattcaatga gtttgcaaaa
      541 atgtgtggta tcagttctac aaacataaac aagcgaactc gctcacgatt tcaggaggcg
      601 ctagcaaacc tagcttccgt tgtgatttct ttccgtgatt caaaaactga acgttttact
      661 gtcacgcacc ttgtgcagaa agcagtgatt gatcctaaga aggacaccgt agagttggtg
      721 ggcgatccct caatgtggga gctttaccgg tatgatcata aaaccttact tagcttgcag
      781 gtgctttcgg ttctcgctaa aaaagaggca gcacaaagcc tgtacatata ttttgaggcg
      841 atgcctgccg gtacgctatt cgtaagcatg aagcgtttgc gtgagcgact tctgttgacg
      901 actcctgtac gaactcaaaa ccagatcatc aggaaggcga tgctggagtt aaaatcaata
      961 ggatatcttg aatatcaaga ggttaagaaa ggtagagata tccagttcca gatttttaaa
     1021 agaagtccaa aacttgctct ggcaaaacac agttgatgct ccgggtatag tacgcacaaa
     1081 agttactttg gatgtaatga ggagtatggg cattcatcca aatgcttttt aatgtttctc
     1141 cggatttgaa cacccttccc tgtttccaaa cttcctatca tttctgatac ccgggttctt
     1201 ctggtattcg ttcgaatgct tttcagaaaa aatcatgctt gctagttgag acaacgtaac
     1261 ctattcattt tgctagttta ataagactca ttcatacata tgcctttaac tacactaccc
     1321 tccatcgtgt tgtattgggc tttaaacgca aatccctata ctccttgtgc cactcggcgt
     1381 atttaca
//
LOCUS       DAFNBJ010000348         1377 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.358, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000348 DAFNBJ010000000
VERSION     DAFNBJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.358"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            50..856
                     /locus_tag="L2X49_005003"
     CDS             50..856
                     /locus_tag="L2X49_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799930.1"
                     /translation="MANNYTQASFTILCSQEQAQMALDAIAYVTDTDVAEGEHLLSKP
                     VSECSLTELLVLGIIQNHPECDPFEPTFGQSESPEDNYELELKAEITGKGLAICHDES
                     INLDHAIAVTTAVLSVFNLPEMVTINAAFVCDRPRENEFGGATIVVTKDTHHYEEGFN
                     FSRLMNEAHDAGVQYALVKVNQYNHEYTYTQCYLMSCKKSESAYDVARHRLASDESTP
                     DNPGEDGVIILSEEDNTSMALHSVTELMPVVYESLSKLLPSLDELCPVTA"
     gene            904..1350
                     /locus_tag="L2X49_005004"
     CDS             904..1350
                     /locus_tag="L2X49_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799931.1"
                     /translation="MTNKILILSASCINEFASDVPQSVFFSIDTSLAKRIRELAAYVK
                     ENDLYAAEFFFYDCHWSETLEEDIEVLMSQAAFIESDSSGQETMLRDVMPSCRTETTS
                     IRVMKESFHLTAVPRHCGDDMMLNTPLIPLAELESNATVLITQQYS"
BASE COUNT          371 a          337 c          334 g          335 t
ORIGIN      
        1 tatctattca cagacagcaa tcacatttaa ttaaataagg agttatgcaa tggcgaacaa
       61 ttacactcag gcatccttca caattctctg ttcacaggaa caggcacaga tggcactgga
      121 cgcgattgcg tatgttacag acacggatgt ggcggaaggt gaacacttgc tgtctaaacc
      181 ggtttcagag tgttccctca ctgaattgct tgtcctgggt attattcaga accacccgga
      241 gtgcgatccg tttgagccga cttttggtca gtcggagtca ccagaagaca attacgagct
      301 ggaactcaaa gcggaaatca ccggtaaagg cctggcaatc tgccatgatg aaagcattaa
      361 cctcgaccac gctatcgcgg taacaactgc cgtgttgtcg gtgtttaacc tgcctgagat
      421 ggtcacaatc aatgccgctt tcgtctgcga ccgaccacgc gaaaacgagt tcggcggtgc
      481 gactattgtg gtgactaaag atacacacca ctacgaagaa ggctttaatt tttctcgcct
      541 gatgaacgaa gctcatgatg ccggtgttca gtatgcgttg gtgaaagtga accagtacaa
      601 ccacgaatac acatacactc aatgctatct gatgagctgc aaaaaatcag aatcggctta
      661 tgacgttgca cgccaccgcc tggcctctga cgagagcacg cccgacaatc ctggtgagga
      721 tggagttatc attctcagcg aagaagacaa taccagcatg gcgcttcaca gcgtcactga
      781 attgatgccc gtcgtgtacg aaagcctgag caaattactg ccttcacttg acgaattgtg
      841 ccccgtgact gcctgataat ccctgattgc caccggaaca gccggtggca tggagagctg
      901 aaaatgacca ataaaatcct gattttgtcc gctagctgca tcaatgaatt tgcgagtgat
      961 gtcccgcaaa gcgtcttctt ttccattgat acgtcgctgg ctaagcgtat ccgtgaactg
     1021 gcggcatacg tgaaagaaaa tgatttgtac gccgccgagt ttttcttcta tgactgccac
     1081 tggtccgaaa cattggaaga ggacattgag gttctcatgt cgcaggctgc gtttattgaa
     1141 agtgacagca gcggacagga aacgatgctc cgggacgtaa tgccctcctg ccgcacggaa
     1201 acaacttcca tccgggtcat gaaagaatca tttcatctga cggctgtacc ccgtcattgc
     1261 ggcgatgaca tgatgctcaa tacgccccta attccgttgg cagagctgga atcaaatgcg
     1321 acggttttaa ttacccagca gtattcgtga gagcgtggag aacataatat atttatt
//
LOCUS       DAFNBJ010000349         1375 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.359, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000349 DAFNBJ010000000
VERSION     DAFNBJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.359"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            133..723
                     /locus_tag="L2X49_005005"
     CDS             133..723
                     /locus_tag="L2X49_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799932.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PIYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLQAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPAEGTSATDLAVELNGMTYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            988..>1375
                     /locus_tag="L2X49_005006"
     CDS             988..>1375
                     /locus_tag="L2X49_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN4799933.1"
                     /translation="MAEKKKKTITGQVLNSIKINKLKCINGLNEIIFKPHALTAILGP
                     NGSGKSTILHAIASIYMPEKGFPGEDHRLMHFFPRSPHAEWNGSDFIVNLTYRKDGVM
                     IENELKNYGKADVRGSRWIQIYARRPL"
BASE COUNT          374 a          324 c          368 g          309 t
ORIGIN      
        1 agccgtgctg atcttctggc atgaactggc cataaatggc tatgcctcaa tgaatgccat
       61 aaagagacag gagctcattg acgccgactt tcagcgcctc agaaacctca tctggccgga
      121 ggatgacagg taatgaccca gagccgccga ccgtcgccac tgcagcggag agtgctgatt
      181 gtgctggctg cgcttgatga aaaacggccg ggccccgtgc tgacgcggga cctggaacgg
      241 gtgctggagc gaagcgggga agccccgatc tacgggccaa acctgcgcgc gtcctgccgt
      301 cgtctggagg atgccggctg gctgcgtacc ctgcgcgccc cgaatctgca gctggctgtc
      361 gagctgacgg acgccggccg ggccgtggca cagcctctgc tgcaggcaga acaggaccgc
      421 ctccgggcag aacagcgtgc ggcagaagtc gtcgtactac cgctggtccc ggccgcaggt
      481 ctgcccgcag aaggcacttc tgccactgat ctggcggtgg agctgaacgg catgacgtat
      541 caggcctgcc gtggggattt tgtggtgcgt ctcgacggca gcacctgtct gcagttatgg
      601 aataaagaag gcagggtggt acgccgggag ggcgatccgc tggaagtggc gcagtggctg
      661 caggcctgtc atgatgccgg aatggaggtt cgcgtacaga taaatgaaag tgccgctccg
      721 taagaacacg cctgtgcacg gtttacacat catcccatgg tggcagccgg ttaactttcc
      781 tgcgccacca gtggatagga gatatgatca gtcataacat taagtctaaa attacagaca
      841 tctcaaaaag tctgtagcat aaaagaggcc tgatgtagaa tactgtgcaa aatagaatat
      901 taaaaggatt tgatatatcc cctgttcaca gttatatgtt tctgaattac atggtgaatt
      961 cccccaacat aatgctagga tttaaaaatg gcagaaaaaa agaaaaaaac tattactggt
     1021 caagtcttga actccattaa aattaacaaa ctaaaatgta taaatggatt aaacgagatt
     1081 atttttaaac ctcatgcgtt aaccgcaatt ttaggtccaa atggaagcgg aaaatcaaca
     1141 atactccatg ccatagcaag catatacatg cctgaaaaag gtttccccgg tgaagaccat
     1201 cggttaatgc atttttttcc acgcagtcca catgctgaat ggaacggcag tgacttcatt
     1261 gtaaatttaa cttatcgtaa agatggtgta atgattgaaa atgaattaaa aaattatgga
     1321 aaagctgatg ttcgaggctc acgatggatt cagatttatg ctcgtcgtcc tttaa
//
LOCUS       DAFNBJ010000350         1369 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.360, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000350 DAFNBJ010000000
VERSION     DAFNBJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.360"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(218..>1369)
                     /locus_tag="L2X49_005007"
     CDS             complement(218..>1369)
                     /locus_tag="L2X49_005007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799934.1"
                     /translation="EGEFFIKAMNILISHLKTNIKEIKLPEIDIIEVSRLATFAPSLQ
                     EISIKEMMRVWNLYSTQSISSLFKTLNVAPETFQSFIDKYGIPADEQIQYPWQDSRIQ
                     QLFDDSGFKETLWQHVMQQRALLKNYFATKGLTDDINARICVVDVGWRGTIHDNIALL
                     YPDIHFTGIYLGLQKFLNEQPSNTSKVAFGPDLNHQLEYPHFLDSVAPIEMITNSPSG
                     SVTGYGLENGKIVAIRSVNDDENSAWHNFTKTFQEGILAGMESFSAAVLSYGITHDVV
                     RGYALNIWDVLISGSNKSLTDAFNNLNHNETFGLGGYVKKNHVPTTFEILSSLWNKNN
                     RAALIEFIKANQWSDGIRKRDNLPSLNKYILALTIDLAVFYKRKFYRKY"
BASE COUNT          411 a          280 c          275 g          403 t
ORIGIN      
        1 gaaacagaag ccactggagc acctcaaaaa caccatcata cactaaatca gtaagttggc
       61 agcatcaccg gctgattcga tgtatttgcg ggaaaaaaat cggcccagat ccgcgaaatt
      121 ttaatcagcg agtcagctgg ggaagaaatg acctgcttat tcgcaccttc cctaacttta
      181 aaaaaaagca tctgtcgatg ccctgaataa aaagtaatta atacttgcga tagaattttc
      241 ttttataaaa aaccgcaagg tcaatcgtca gtgctaaaat gtatttattt agcgagggca
      301 gattgtctct tttacgtatg ccatcgctcc attgatttgc tttaataaat tcaatcaatg
      361 ctgcacggtt gtttttattc cataaagagc ttaatatctc gaaggtcgtg ggtacatggt
      421 ttttcttaac gtatccacct aagccgaatg tttcgttatg atttaaatta ttaaaagcgt
      481 cagttagaga tttattacta ccactaatta gcacatccca aatatttagg gcgtagccac
      541 gtacaacgtc gtgagtaatg ccataactca acactgcagc ggagaaggat tccataccgg
      601 ccaggatccc ttcttgaaat gtttttgtga aattatgcca cgcgctgttt tcgtcgtcgt
      661 taacactgcg aatagcgact atcttaccat tctccagccc atagcctgtt acgctacctg
      721 aaggggagtt ggtaatcatt tcaataggag caacggaatc aagaaaatga ggatactcca
      781 gttggtgatt caaatctggg ccaaaagcaa cttttgatgt gtttgatggt tgctcgttga
      841 gaaatttctg caggcccaga tatatgccgg taaaatgaat atcaggataa agaagagcaa
      901 tattatcgtg aatggtgcca cgccagccga catcgactac gcaaatacgc gcattgatat
      961 catcagttag accttttgtc gcaaaatagt tcttcaaaag agctctttgc tgcatcacat
     1021 gctgccagag cgtctctttg aaaccagagt cgtcaaaaag ttgctgaata cggctgtcct
     1081 gccatggata ttggatctgt tcatctgcag gaatcccata tttgtcgatg aaactctgga
     1141 atgtttcagg ggcaacgtta agggtcttaa ataaagagct aatggactgt gtgctatata
     1201 aattccaaac acgcatcatc tcttttatgc taatttcttg tagagacggc gcgaaggtag
     1261 ccagacggct gacttctata atatcgattt ctggtaactt tatttcttta atattagtct
     1321 tgagatgact aatcaaaata ttcattgctt taatgaaaaa ctcgccttc
//
LOCUS       DAFNBJ010000351         1328 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.361, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000351 DAFNBJ010000000
VERSION     DAFNBJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.361"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            71..475
                     /gene="stpA"
                     /locus_tag="L2X49_005008"
     CDS             71..475
                     /gene="stpA"
                     /locus_tag="L2X49_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBN4799935.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(522..1046)
                     /gene="ygaP"
                     /locus_tag="L2X49_005009"
     CDS             complement(522..1046)
                     /gene="ygaP"
                     /locus_tag="L2X49_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBN4799936.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPFPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1056..>1328)
                     /locus_tag="L2X49_005010"
     CDS             complement(1056..>1328)
                     /locus_tag="L2X49_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4799937.1"
                     /translation="SAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGELTRITGLSAS
                     ATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          355 a          321 c          316 g          336 t
ORIGIN      
        1 ataataagtg atcgcttaca ctacgcgacg aaatactttt ttgttttggc gttaaaaggt
       61 tttctttatt atgtccgtaa tgttacaaag tttaaataac attcgcaccc tccgtgcgat
      121 ggctcgcgaa ttctccattg acgttcttga agaaatgctc gaaaaattca gggttgtcac
      181 taaagaaaga cgtgaagaag aagaacagca gcagcgtgaa ctggctgagc gtcaggaaaa
      241 aattagcacc tggctggagc tgatgaaggc tgacggaatt aacccggaag agttattagg
      301 taatagctct gctgctgcac cacgcgctgg taaaaaacgc cagccgcgtc cggcgaaata
      361 taaattcacc gatgttaacg gtgaaactaa aacctggacc ggtcagggcc gtacaccgaa
      421 gccaattgct caggcgctgg cagaaggtaa atctctcgac gatttcctga tctaattact
      481 gaggccggat gtcgctgcta gggcgcgtcc ggcgtcgctt ctcaagctcg ttggttccac
      541 ggcatcttat ctaacaacct tgccattcca caaaaaccgc tgattcctgc aaacagtaac
      601 ccggcaccaa caaagccact taataagaag aaaccgctat ttacggtata acccagtaca
      661 acgccgatta atattaaacc acccgcagcg atctgcacct ggcgcattaa cggaaagggt
      721 tgagatttat ttaccgctac tggcaatcca gctcttttcc agccatcaat cccatcttcg
      781 agtaaaaata tttctgcggg ggcggcaatc gctgctaatt tatcggcatt attactggtg
      841 cgtttacctg cctggcagtg gaatataatt tgctcgtgac gtaatttagc cggaagacct
      901 gactgttcca gcacggataa tggagccaga tctgcttcag gaatatgttc acgaagatat
      961 tcatcagcat cacgaatatc gattaacttt gcgccgcgtg cgattaattc ttgtgcatca
     1021 tgcggcgaaa tggttgtcaa agccatagtg actccttacg gacaatagac atttttcagg
     1081 gtggcgataa tggcatttac cgcctcattt ttaatggaat atagaatgcg ttgggcatcc
     1141 cgttggctgt cgataagccc ttcgtcccgc atacgagcga gatgctgtga tgtcgcagag
     1201 gcactcagtc cggtaatgcg cgtcagctct cccgcgctgg tgccggggga accgctaagc
     1261 atgcacagaa tcagcaatcg tttagggtgg ctcattgctt tcaataaggc cgctgcctgt
     1321 tcggcact
//
LOCUS       DAFNBJ010000352         1307 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.362, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000352 DAFNBJ010000000
VERSION     DAFNBJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.362"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..466
                     /locus_tag="L2X49_005011"
                     /pseudo
     CDS             1..466
                     /locus_tag="L2X49_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284686.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(528..821)
                     /locus_tag="L2X49_005012"
     CDS             complement(528..821)
                     /locus_tag="L2X49_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBN4799938.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTIENSLKENQNKRSGESQAIV
                     DARQFFSKHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
     gene            complement(823..>1307)
                     /locus_tag="L2X49_005013"
     CDS             complement(823..>1307)
                     /locus_tag="L2X49_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HBN4799939.1"
                     /translation="DVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEHQHRNLN
                     PNTEPVNILLSNNFIRINKNITLSDLTHFLISNLHTQNVYSTQEPLTHDEIDILRLCV
                     SYSLKQIAIIKGIDYKTISYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQSCVRES
                     "
BASE COUNT          434 a          216 c          239 g          418 t
ORIGIN      
        1 acaagaagca agaaggaaat ccagaaatta agtctaaacg cagagagcgc atcaggaaat
       61 tctttctgag caattgaagt ctgatgtcag taatagtcgt ttgatgattg ccaaccctac
      121 tcacattgca ataggaatat actttaagcc acatctttca cctattccat tgatttctgt
      181 aagagaaact aatgaggtag ccttagccgt aaggaaatat gcaaaggaaa tcggaatacc
      241 aattatcaca gataaaaaat tagcacgaaa aatttatgct acccatcgtc gctacgatta
      301 tgttagcttc gaaaatatag atgaaatatt acgtcttctg ctgtggcttg aagatgtgga
      361 gaatgctgga caacctgttc cagatgaaca gctctcttca gaagataaat tcattgaagg
      421 tgaagagaaa gaaatcgaga ataaagacga taatttaaaa aattaaacat ctgtcgtagt
      481 cagaaattta taagaatcta cacgcaaaaa ccgtgtagat tcattattta atgagttaaa
      541 cgatcttgtg aaatgacaag caattgatac atactcatct ctttataagc tggaactgta
      601 gtaacctgtg caaattcgcg atatagcttt tgctccagtt ctgctgaaga tatatattcc
      661 gggtgtttgc taaagaactg tcttgcatct actatcgctt gggattcgcc acttctttta
      721 ttttgattct cctttaatga attttctatg gtgcatgcag agagcaacga tatcagcgcg
      781 atggttatta tgatgttact attatatcct actaatgaca tatcaacttt ctcttacgca
      841 agattggagt ttaaaataat gtttatccca ttcacagagt gctataaaca actcaacagt
      901 accttttatg ttaagtttat ttagggctct tattttgtga tatgatattg ttttataatc
      961 aattccttta ataatggcta tttgttttaa cgagtaagaa acacataacc gcaggatatc
     1021 tatctcatca tgggtaagtg gttcttgtgt ggaatacaca ttttgtgtat gaagattcga
     1081 aatcaggaaa tgcgttaaat cagataatgt aatattctta ttgatcctga tgaaattgtt
     1141 gcttaaaagg atgtttacag gttctgtatt tggattgaga tttcgatgtt gatgctcatc
     1201 aataacaacg aacgaatgcc caagatcaac caacgcaact aaatcgctgt aatattcttt
     1261 ttgggatgct aataatgcat tagtgcctac agtgaagtag acatcac
//
LOCUS       DAFNBJ010000353         1281 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.363, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000353 DAFNBJ010000000
VERSION     DAFNBJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.363"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(464..1096)
                     /locus_tag="L2X49_005014"
     CDS             complement(464..1096)
                     /locus_tag="L2X49_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBN4799940.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          343 a          267 c          264 g          407 t
ORIGIN      
        1 aggcgtaata tcttctataa ataacccgca aaatatcgtc gtagctgcac tcgatatttt
       61 taaaatcctg tatcaagtgc tgaaaatcat ttcatcgcca cttccaattc ctttctgcat
      121 ggtaagtagt gaggacgaaa agaagaatat gataaatatt gattcgtggt aatcagagag
      181 agtcgcgaca tttgtgatgt tattcttaca actgtaagaa gcgccggtga tttacaagta
      241 agggtatgat tacgcttttt aaagcgtacc ataaaacata acataataaa tgatttgtga
      301 tgacacggga ttattgggat ttactttgag gaagataaaa tgcgtgttta tagaaccgcg
      361 agcgccaaat aattttatga ccacaacctg tcgtattgtg gtcataaaaa attgtcggct
      421 ggagggagtt aaatccttta cctcaatata ctgcctgtca gcattagttc acaaacagtg
      481 cttcgctgtc gacgatgatt ccatcttccg cacctttaag cgccaggttt gcctcgtagt
      541 attgcttttt atcaagcagt ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg
      601 gcatcagata ttggttttcc attacgccaa caccggccag gcgaagttct tccattgcgc
      661 cctttttatc ccctttcgcc attttttcat tggcaatttt tatcgcggct tctttttcgg
      721 gagttgctac ataactctca gaaattccga cagatgcatt aatgacaata tattggtcat
      781 cattgagatt ggtcttttta cctggcttag cgaactttgc ccattcggta ctgtcatctg
      841 acagcaaagc ggaagcttca ttggtcagtt cttttgcctt ttctggatcg ccatgaaaca
      901 gagccaggcg cgctacctgc acgtcacgca tcgcatataa accttgttca gagatacgct
      961 ctgctgctgc cgttttctgg gtttgctgaa cttgctgagt ttgctgttgt gccgctaccg
     1021 gtgcattatc agcagcccat acagttgaag atgccagaat tgctgtgacc atcgtagcca
     1081 taattaaacg tttcatactt acctcattct ttaacagttt tagaaaacgc cgccatcata
     1141 ttgggtgagc atgaaatgag aaatcagaga gcaacggtca ttttgaaagg gttatcttac
     1201 agttgtagtt attatcgcaa gaagtatttt agtcttacaa ttgtaggaat ctgcttcctg
     1261 tcgcagagaa ctattcttat g
//
LOCUS       DAFNBJ010000354         1275 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.364, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000354 DAFNBJ010000000
VERSION     DAFNBJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.364"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            48..1268
                     /locus_tag="L2X49_005015"
     CDS             48..1268
                     /locus_tag="L2X49_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
                     /protein_id="HBN4799941.1"
                     /translation="MDEKSLYAHILNLSDPWQVKSLSLDENAGSVTVTIEIAENTRLA
                     CPTCGKSCSVHDHRHRKWRHLDTCQFTTIVEADVPRIMCPEHGCLTLPVPWAGPGSRY
                     TLLFESFVLSWLKISTVDAVRKQLKLSWNAVDGIMTRAVKRGLARIKKPLSARHMNVD
                     EVAFKKGHRYITVISDRDGRALALTDDRGTESLAGYLRTLTDGQLLAIKTLSMDMNAG
                     YIRAARIHLPSAVEKIAFDRFHVAKQLGEVVDKTRQNEHPHLPVESRHQAKGTRFLWQ
                     YSDKWMTESRQEKLMWLRAQMKLTSQCWALKELAKDIWNRPWSEERRSDWQRWLALAA
                     NSDVPMMKNAAKTIGKRLYGILNAMRHSVSNGNAEALNSKIRLLRIKARGYRNRERFK
                     LGVMFHYGKLNMAF"
BASE COUNT          325 a          312 c          358 g          280 t
ORIGIN      
        1 ggctgaaggc atgataatgg tgtatttaat cgccagaggt caccgccatg gacgaaaagt
       61 ccctctacgc tcatattctc aacctgtccg atccgtggca ggtaaagtcc ctttctctcg
      121 atgaaaatgc cggttctgtt actgtcacta ttgagatcgc tgaaaacacc cggctagcct
      181 gtccgacctg cggtaaatcc tgttctgttc acgatcaccg tcatcgtaaa tggcgccatc
      241 ttgatacctg ccagttcacc actattgttg aagccgatgt tccacgaatt atgtgtccgg
      301 agcatggctg cctgacgttg cctgttccgt gggctggccc cggaagccgg tatacgttgc
      361 tattcgaatc gttcgttctc tcatggctga aaatcagcac cgttgatgct gtcaggaagc
      421 aacttaagct cagttggaat gcggttgacg gcattatgac ccgggcagtt aagcgaggtc
      481 ttgcccggat aaaaaagcca ttatccgccc gtcatatgaa tgtggatgag gtcgccttta
      541 aaaaaggaca tcgttacata acggtgatct ccgatcgcga tggtcgggcg ctggccttaa
      601 cggatgatcg cggcacagag agtcttgccg gctatcttcg cacgctcact gatgggcagt
      661 tgctggctat caaaacgctc tcaatggaca tgaacgcggg ctatataaga gcagcgcgta
      721 tccacttacc cagtgcggtt gagaaaatcg cctttgaccg cttccatgtg gcgaagcaac
      781 tgggcgaggt agttgataaa acccgtcaga atgaacatcc gcacctccct gttgaaagcc
      841 gacaccaggc aaaaggaacc cgcttcctgt ggcagtacag cgataagtgg atgaccgaat
      901 cccggcagga aaagctgatg tggctgcgtg cacagatgaa gctgacgagc cagtgctggg
      961 cgctgaaaga gctggcaaag gatatctgga acaggccatg gagcgaggaa agacggagtg
     1021 actggcagag atggttggcg ctggcggcta acagtgacgt tcccatgatg aaaaatgccg
     1081 cgaaaacgat aggaaaaagg ctgtacggga tcctgaatgc gatgcgacac agtgtctcaa
     1141 acggaaatgc ggaggcactt aacagcaaga tcaggctgct gaggataaaa gccaggggat
     1201 accgaaaccg ggagcgcttt aaactggggg tgatgttcca ctacggaaag ctgaatatgg
     1261 cgttctgagc cttcc
//
LOCUS       DAFNBJ010000355         1270 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.365, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000355 DAFNBJ010000000
VERSION     DAFNBJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.365"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..816
                     /locus_tag="L2X49_005016"
     CDS             <1..816
                     /locus_tag="L2X49_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799942.1"
                     /translation="YLNAVFDELDFEMNREYNQPDFLLSKFGVEIAVEAVSIAEAEAP
                     LERKVINDEQMDELRKHVLNVMPFKFARSLLKKVRHCPEPEKVHYWELNHTKNKPFVI
                     AMQDYSKRMSMAFSSEALHSYLYGIDIESGISIERHTDENRSIKSNFFGSEQNNYVSA
                     VLLTTQATIPKFNRMGILAGVEASGFKVYVSGVKTDQDAAPHPFSADVSDPNYQEPWC
                     TAMYMYHNPNAIHPVDYQLFPNVVHVFKKDEHFEEYIPRNYILQSTTMICKTE"
BASE COUNT          404 a          259 c          254 g          353 t
ORIGIN      
        1 tatttgaacg ctgtatttga tgaactcgac tttgaaatga atagagaata caatcaacca
       61 gattttctgc tatctaaatt tggagtggaa attgctgtag aggctgtaag cattgctgaa
      121 gcagaagccc ctcttgaaag aaaagtcatt aacgatgaac agatggatga attaagaaaa
      181 catgttctaa atgtcatgcc ttttaagttt gcaagaagtt tattaaaaaa agtccggcat
      241 tgccctgagc cagagaaagt tcattactgg gaactcaacc atacaaagaa caaaccattt
      301 gtcatagcaa tgcaggatta ttcaaaaaga atgtccatgg ccttctcaag cgaagccttg
      361 catagctatt tgtacgggat tgatattgag tcaggcattt ctattgaaag acatacagat
      421 gaaaatagaa gtatcaagtc gaactttttt ggctcagaac aaaataatta tgtttctgcc
      481 gttttactca ctactcaagc aaccatacct aagtttaacc ggatgggaat tcttgcaggt
      541 gtagaggcga gtggattcaa agtgtacgtc agtggtgtta aaactgatca ggatgcagcc
      601 ccacatccat tttcagccga tgtaagtgac cctaactatc aagaaccatg gtgtacagcc
      661 atgtacatgt accataatcc aaacgctata caccctgttg actatcaatt attcccaaat
      721 gtagttcatg tatttaaaaa ggacgaacac tttgaagagt acattcccag aaactatatt
      781 ctccaatcga ctactatgat atgtaaaacg gaataaattg tggtttacta aaagctaata
      841 aatgattaat gcgtttttcc gtattaatcg tttattatca aagcaattca ccacgaatcc
      901 acacctgtac ttccgatagc cgataagcta ccgctgaaga tccaattttg atgcgctggt
      961 ttttcggggg gattactctg gttcttcatc gaagagcgga gtaccggagt tacttatcgt
     1021 cagagtgcta cgatttcgct gaatacagaa cttcaacttg ataagtgcag tttctttaag
     1081 cccccctgca aaccaaccat caacttttgg agggctcttg cataccaacg tccgctcttt
     1141 gcacagagcg gacgatatca ctgagctgaa ggtccgctga gagcgaggaa cggacattac
     1201 taacagcatt ctgtgtgaat caccagggag caggtcagcg ccactactct atataatcgc
     1261 taacgatgac
//
LOCUS       DAFNBJ010000356         1208 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.367, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000356 DAFNBJ010000000
VERSION     DAFNBJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.367"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>1208
                     /gene="rhsB"
                     /locus_tag="L2X49_005017"
     CDS             <1..>1208
                     /gene="rhsB"
                     /locus_tag="L2X49_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HBN4799943.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEMRRKYDEYGR
                     LIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLTFTDCSGYVTRY
                     DHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEYNAAGDLTTVIAPDG
                     SRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWRYDERGWL
                     TDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRC
                     IPDSLPAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAYTPAGQ
                     LQSQHLNSLL"
BASE COUNT          286 a          374 c          360 g          188 t
ORIGIN      
        1 gggactcatc acgcgcatca ccacgccgga tggcagggca tcggcgtttt actataacca
       61 ccacagccag ttaacgtcag ccaccgggcc tgacgggctg gaaatgcgcc gcaaatatga
      121 tgagtacgga cgtctgattc aggaaaccgc ccctgacggc gatatcaccc gctaccgtta
      181 tgataatcca cacagtgacc tcccctgcgc aacggacgat gccaccggca gccggaaaac
      241 catgacgtgg agccgttacg gtcagttgct gaccttcact gactgttccg gttatgtaac
      301 ccgctatgac catgaccgtt ttggtcaggt gacggcggtg caccgcgagg aaggcctgag
      361 ccagtaccac gcatacgaca gccgtggaca gttaactgcg gtgaaagaca cgcagggcca
      421 tgaaacgcgg tatgaatata acgccgccgg tgacctgacc accgtcattg ccccggacgg
      481 cagcagaaac gggacacagt acgatgcgtg gggaaaggcc gtccgtacca cgcagggcgg
      541 gctgacccgt agtatggaat acgatgctgc cggacgggtc atccgcctga ccagtgaaaa
      601 cggcagccac accaccttcc gttacgatgt actcgaccgg ctgatacagg aaaccggctt
      661 tgacggccgc acacagcgtt atcaccacga cctgaccggc aaacttatcc gcagcgagga
      721 tgaggggctg gtcacccact ggcactatga cgaagcagac cgcctcacgc accgcaccgt
      781 gaagggtgaa accgcagagc ggtggcggta cgacgaacgc ggctggctga cagacatcag
      841 ccatatcagc gaagggcacc gggtgacggt gcattacggg tatgatgaga aaggccgcct
      901 gaccggtgag cgtcagacgg tgcatcaccc gcagacggaa gcactgctct ggcagcatga
      961 gaccagacat gcgtacaacg cgcaggggct ggcgaaccgc tgtataccgg acagcctgcc
     1021 cgccgtggaa tggctgacct acggcagcgg ctggctgtca ggcatgaaac tcggcgacac
     1081 accgctggtg gagtacaccc gcgaccgcct gcaccgggaa acgctgcgca gcttcggtcg
     1141 ttatgaactc accaccgctt acacccctgc cgggcagtta cagagccagc acctgaacag
     1201 cctgctgt
//
LOCUS       DAFNBJ010000357         1188 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.368, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000357 DAFNBJ010000000
VERSION     DAFNBJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.368"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..547
                     /gene="corA"
                     /locus_tag="L2X49_005018"
     CDS             <1..547
                     /gene="corA"
                     /locus_tag="L2X49_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBN4799944.1"
                     /translation="LFETKIEQLADEIENIYSDLEQLSRVIMEGHQGDEYDEALSTLA
                     ELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQAREILRDIESLLPHNESLFQKV
                     NFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSYGMNFEFMPELKWSFGYPGA
                     IIFMILAGLAPYLYFKRKNWL"
     gene            complement(590..970)
                     /locus_tag="L2X49_005019"
     CDS             complement(590..970)
                     /locus_tag="L2X49_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HBN4799945.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(984..>1188)
                     /locus_tag="L2X49_005020"
     CDS             complement(984..>1188)
                     /locus_tag="L2X49_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799946.1"
                     /translation="NCQRLRDSGFIYIKTYVFGTLAVYIISIITMIAEDFACSGNGSM
                     IFLICYFSTFSMLMLAPTDSSKQ"
BASE COUNT          352 a          250 c          260 g          326 t
ORIGIN      
        1 tctgttcgaa accaaaatcg aacagttggc agatgaaatt gaaaatatct atagcgacct
       61 ggagcagttg agccgggtga ttatggaagg gcatcagggc gatgagtacg acgaggcgct
      121 ctccactctg gcggaactgg aagatatcgg ctggaaagtg cgcctgtgtc tgatggatac
      181 ccagcgtgcg ctcaacttcc tggtgcgtaa agcgcgttta ccgggtgggc aactggagca
      241 ggcgcgtgaa atcctgcgag atatcgaatc cctgctgccg cataacgaat ccctgttcca
      301 gaaggtgaac ttcctgatgc aggcggcaat gggttttatc aacatcgagc agaaccgcat
      361 catcaaaatt ttctcggtgg tatccgtcgt gttcctgccg ccaacgctgg ttgcttccag
      421 ttatgggatg aactttgagt ttatgccaga actgaagtgg agtttcggct accctggcgc
      481 gattatcttt atgatcctcg cgggcctggc accgtatctg tactttaagc ggaagaactg
      541 gttgtaaaaa acgagagcgg tggcttagtc tggctaagcc acctgttatt caaaggctcc
      601 aggtatttaa cccttttacc tctttctcat agaaccattt gttcgtgtta acagcaacat
      661 aggctgctac ggcaattccc agaatgttaa cgccaattaa tgcaccaaca aatagagata
      721 gaatgccaag taatagaact ataattgctt ttttccataa gcccagaaca aataaataga
      781 tccaggaaca gaagaaagca atgaaattca tttgaatagt taatcgttgt cttactttta
      841 atgctttaaa tgctgcttta tattctggtg ttgctcccca gaatccagga aagccatgtt
      901 gatcataaaa attaaatcgg tatttccatt tttcacttaa tgaaccatcg ttcatatatt
      961 ccttactcat aaatactcca taactattgt tttgatgaat cagtaggtgc aagcattagc
     1021 atactgaaag tggaaaaata acaaatcaaa aaaatcatcg aaccattgcc tgaacaggca
     1081 aaatcttcgg ctatcattgt gatgatagag atgatatata ctgctaatgt accaaaaaca
     1141 taagttttta tatagatgaa accactatca cggagtcgct ggcaattc
//
LOCUS       DAFNBJ010000358         1184 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.369, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000358 DAFNBJ010000000
VERSION     DAFNBJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.369"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            53..655
                     /locus_tag="L2X49_005021"
     CDS             53..655
                     /locus_tag="L2X49_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBN4799947.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDITGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNDIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLNGLVMHVEKGKVYLDGEFVNH"
     gene            complement(694..1119)
                     /gene="nudI"
                     /locus_tag="L2X49_005022"
     CDS             complement(694..1119)
                     /gene="nudI"
                     /locus_tag="L2X49_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBN4799948.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          326 a          266 c          252 g          340 t
ORIGIN      
        1 gctcaatttc acggtaattg tctggttgcg cttgtctata ggtggagttt acgtgttagc
       61 tttttgccgc tcatcattga agtcaaaaaa atatttcatc attttactgg cgctcgctgc
      121 aattgccgga ctgggtactc atgccgcctg gagtagcaat ggtttgccac gtatcgacaa
      181 taaaacattg gccagactgg cacagcagca cccggttgtc gttttgtttc gccatgctga
      241 acgttgcgat cgttcaacca atcaatgctt gtcagatata acaggtatta cggttaaagg
      301 tacccaggat gcccgtgaac tgggcaacgc ttttagtgct gatatccctg atttcgatct
      361 ttattccagt aataccgtcc ggaccattca gtcggcgacc tggttttcag cgggtaaaaa
      421 gctgacggta gataaacgat ttcttcagtg cggtaatgat atttatagtg caattaaaga
      481 cttacaaagc aaagcacctg ataaaaatat cgttattttt acccataatc attgcctgac
      541 atatattgct aaagataagc gtgacgcgac atttaaacct gattatctga atggtttagt
      601 catgcatgtg gaaaaaggca aagtttatct ggatggggaa ttcgttaacc actaaatttt
      661 caatctgaca gccagtaatg gctgtcatca ttgttacaga agacctttca aacgtaacgt
      721 ttttcgggtg gcaacgttca aatcatagtg caccagatct tcaggtttta cccacgcgta
      781 gtcctgaaac tcttcgttta ttttcacttc tcggttggca gaaacgcagt caaaaatcag
      841 gtaaatcata taaatctctt ccttgcgacc atctgcatac gtcttggtgc gaatatcatc
      901 gctgaaggtc cacggcgtga tttctgtcaa aagcagctgt tctcccagtt cttcgcgaat
      961 ttcgcggcgt agtgcctctt caattcgttc gccaggctct acgccgccac ccgaaagcgc
     1021 ccattgaccg gggaaaacgc cgcgatcgtc ggccatttta cacagcaaat aagcaccatc
     1081 attttgaatc aaagggcata caatagtccg ttgtcgcaca gggatattcc ttataaataa
     1141 aaagaaacca tgagaatacg gtaactcaca aatatgtgaa agtg
//
LOCUS       DAFNBJ010000359         1174 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.370, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000359 DAFNBJ010000000
VERSION     DAFNBJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.370"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(56..1000)
                     /gene="leuO"
                     /locus_tag="L2X49_005023"
     CDS             complement(56..1000)
                     /gene="leuO"
                     /locus_tag="L2X49_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBN4799949.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          353 a          268 c          242 g          311 t
ORIGIN      
        1 caataaaaaa tacagaataa accagacatt catgtctgac ctattctgca ttcagttagc
       61 gtttgcaaat tgagactaat tgctcttcca tccactgatg gcctttatcg cgcccggcag
      121 cttcatgcca ggagagataa caggttctgc tattttgttt taacggcagc ggtaatacct
      181 gtaattctaa ggattcagcg aactcttcag ccagccaacg cggcgcaata gcgaccaaat
      241 gcgtttgcga caccacgcta agtacgctca tcattgccat gccctgatac gcgatactgg
      301 cttgcttatc taccgtgtca taccaaggtt gactaaatga cgcgaaacga tcgagcgaaa
      361 ccgccgcatg ttgttcgtta taaacatcat gtttcagtaa cgggccctta attgttggat
      421 gatttttgct ggctaccagc accatttcat ctttaaataa tggcacgctg gtaaattcag
      481 gacgatggaa gtcttcataa ctaatcacaa actccgtttc ctgataacgc agctgatgtt
      541 cagtgttctg atttaatgaa gacttgaaca taacatgtat atttggcgca atctgctcaa
      601 tgtgattata aatctgcgag gtcagaatgc tgtctaacgg gctgcaaaca caaagatgaa
      661 atacacgttc actgctcgcg ggttcaaaac ctgaaccagg caattcattt tgtactagtt
      721 gcaatgcctg acgaactgag ccaaaaagtt gaaatgcgcg agcagtcggt tgaataccac
      781 ggccataacg aacaaaaagc tcgtcattaa acatcacctt caggcgtgca acagcgttac
      841 tgaccgcagg ttgcgacata cccagaacat gagcggcacg ggtaatgttt tgctcctgca
      901 tcacggcatc gaaaacggtt aataagttga gatcgaccat gcgtagctgt ggcttgctta
      961 actccgccgt ctctgaatga tctgtttgta cctctggcat acttaactcc actgtcacac
     1021 ttaactccct ttcccttatt ggaatgcaga gaaataatcc tgaagaatat gatttatcat
     1081 gcatataaaa taagaaaaag caaaatgagt aaaattcgga aaacataaag atacgggttc
     1141 tgataaaaac agaatccatt gccgtggaat gagt
//
LOCUS       DAFNBJ010000360         1154 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.371, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000360 DAFNBJ010000000
VERSION     DAFNBJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.371"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..238)
                     /locus_tag="L2X49_005024"
     CDS             complement(<1..238)
                     /locus_tag="L2X49_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBN4799950.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPAT"
     gene            326..1081
                     /locus_tag="L2X49_005025"
                     /pseudo
     CDS             326..1081
                     /locus_tag="L2X49_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          288 a          280 c          311 g          275 t
ORIGIN      
        1 ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata taatcccggt
       61 attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc tcagattttg
      121 cctgcaaata acgtttaacg gtattgcggg agatccccag ttctctggca atcgcccggc
      181 tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa gtgaccataa
      241 gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg ttgggtgtgg
      301 atcactattg caccgttcgt gacaagaagg ttttgagctt gccgtggtta cccccagaca
      361 agctcgtgac tttgcccgca ccatgggata cctcgcgaaa actgatagta ttgatgccag
      421 agtgctttac cagatggctg aggttctcaa ccggcatccg gagcgggaac gctttatcag
      481 agcactgccg gatactgaat gtcaggttct cactgcaatg gtagttcgta ggcggcagtt
      541 gattacgatg ctggttgccg agagaaaccg gttgcaccct gctcatccat agaacaggaa
      601 aagcattaat atcatcatca aagcactgga agacgagctt gcgcgactag atgctgacat
      661 gaacagccat atccatagcc acttcaaaga gcttgctaag cgactgagca atattaaagg
      721 tgttggcacc atgacggctg ctgcgctgct ggcggaagta cccgaacagg gtaacctctc
      781 caggcgtgaa atcagtgcgc ttgtcggtat ggttccagta aaccgggact cgggcacgat
      841 gagagggcga cgaaccatct ttggcggcag ggctggtgtg cgaacagccc tgtacatggc
      901 agcgcttgtc gcaacccgct ttaacccggt aataaaggtg ttttacatgc gactgcttgc
      961 cgcaggaaaa cccaaaaaag tggcgctggt tgcctgtata cgcaagctgc tgactatcct
     1021 gaacgcaatg ctcagaaaga acgaagaatg gaatgaatcg taccatcacg ttgctccata
     1081 atttttacgt tcaagacagt tgctgagctg tccggggaag actgaaacct ccggtctggc
     1141 agtgtgtgca gtac
//
LOCUS       DAFNBJ010000361         1145 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.372, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000361 DAFNBJ010000000
VERSION     DAFNBJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.372"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..331
                     /locus_tag="L2X49_005026"
     CDS             <1..331
                     /locus_tag="L2X49_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799951.1"
                     /translation="SNNMAIYWNSRASLFCSTELNSKSQSPALGLGHEFAHAQYYLLD
                     KENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTM
                     KITGTPE"
     gene            343..792
                     /locus_tag="L2X49_005027"
     CDS             343..792
                     /locus_tag="L2X49_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799952.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYAGKIIDLLQMKYFRKEKYNNIRWGVSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          388 a          148 c          263 g          346 t
ORIGIN      
        1 ttcgaataat atggcgatat attggaatag tagagcatct ttattttgtt caactgaatt
       61 aaactcgaaa tcacaatcgc ctgcattagg tttgggacat gagtttgctc atgcgcagta
      121 ttaccttctt gataaggaaa atttcatggc tcttttaagc cgaacagata agaaatatga
      181 gaataaagaa gaggcaaggg ttattacaat aattgaatcg cgtgcggcga aaactttagg
      241 tgagtgtacc agaggagctc atagtggact gcctttttat cgggtggatg gaccgttgca
      301 gacgatgaaa ataacgggta cgccagaata gtgaggtttt ttatgatgaa atatttgatg
      361 gttttattgt ctctattttc tggttcggta ctggggatgg ggcgagtgaa tgaattgtgt
      421 ggaatagata gcgtgaaaac gatagagata attaatcttc catcgtatgt taccacgtta
      481 gttccgctta gcaaggaagg tcttaatgaa atatacagat ataaagtggt ggtgaatgaa
      541 attagcgatc tttatgctgg taagataatt gatttattgc aaatgaagta tttcagaaaa
      601 gaaaaatata acaatataag atggggggtt agtatcattt cgaaaggtaa taataaatgt
      661 gaaatttatt ttgatgcttt tggtgaatgt ggaagtgtga atggcattaa cgtgtgtttt
      721 gaaaaaaatg aaatgatagg ttggattaaa aaagaaatcc ctttgctgtc gcaaaaaata
      781 ggtgggcttt aaatggtagg gcaatattca aagacagtat aggtaactat ttgtatggcc
      841 atgtaaggta aaagaaaagg aataaaccat gagcggaaaa ccggcggcgc ggcagggcga
      901 catgacccgc agggaaagat catcagaagt tcactttttg tactaaataa ttcgcatttt
      961 atgtttaaaa attgagatat tcctcactac ctgaagctgt tttttattgc ttatacatga
     1021 tcaaatactc cttacataaa taaggtgaac aaatggaact taaaaaattg atggaacata
     1081 tttctattat tcccgattac agataagcct ggaaagtaga gcataaattg tcaggcatcc
     1141 tacta
//
LOCUS       DAFNBJ010000362         1128 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.373, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000362 DAFNBJ010000000
VERSION     DAFNBJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.373"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>1128
                     /gene="espL4"
                     /locus_tag="L2X49_005028"
     CDS             <1..>1128
                     /gene="espL4"
                     /locus_tag="L2X49_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HBN4799953.1"
                     /translation="INNTAQTNEHQTLSELFYKELEDKFSGKELATPLLKSFSENCRQ
                     NGRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPIFEEYLMEILPQRS
                     DTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAAQYINDIYQNVDYK
                     PHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKAYFDKMESNG
                     ISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMSESKEDIKHY
                     SLMDFMNVDYSLLKWSNDHIINQSVAIIPALPKEQLLMLKGSVDEITPPLSPATMNLL
                     MAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSGHMNVINT"
BASE COUNT          405 a          216 c          200 g          307 t
ORIGIN      
        1 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
       61 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
      121 agaactgtag acaaaatggt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
      181 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
      241 cgacacttac tcaaaccatt gccccaatat ttgaagaata cctaatggaa attttacctc
      301 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
      361 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgaccc caaaaccgta
      421 tagtatgccg acacattgct gcacaatata ttaatgatat ttatcagaat gttgattaca
      481 aaccccatca agatgattac tcttcagctg aaaaatttct cactcacttc aacaaaaaat
      541 gcaaaaacca gactttggcg ttgatttcca gccgtcctga ggggcgttgt gttgctgcct
      601 gtggtgattt cgggctagtt atgaaggcat atttcgacaa gatggaatca aatggcatca
      661 gtgttatggc agccatatta ctggtggata accatgcttt gacggtccgg ctaagaataa
      721 agaacacaac tgaaggatgt acccattacg tggtttcggt ttatgatcct aatgtcacta
      781 acgataaaat aagaattatg agcgaaagca aagaggatat taaacactat tctctgatgg
      841 attttatgaa tgtagattat agcctcctga aatggtcaaa tgatcatatt attaaccaat
      901 ctgttgcaat aattccagca cttccgaaag aacagctatt gatgttaaaa ggatctgtgg
      961 atgaaataac ccctccatta tcaccagcaa cgatgaattt gctaatggca attggtcaga
     1021 atcaccaact tacgcaactg atgattcagc tccagaaaat gccagaacta catagaacag
     1081 aaatgttgac tgcctataat agtggacata tgaacgttat taatacta
//
LOCUS       DAFNBJ010000363         1118 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.374, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000363 DAFNBJ010000000
VERSION     DAFNBJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.374"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..737)
                     /locus_tag="L2X49_005029"
     CDS             complement(<1..737)
                     /locus_tag="L2X49_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799954.1"
                     /translation="MLKSFCLNFDGMYLSIVSFLSEKFNLKRVGKMELESKEDYEETT
                     HHNVASSVVTATHDNLDGREFFELCSNPALVFSFETHKKFNELPVDSKESPLDLFIAN
                     TKQLHLLRSGDISNTLSHLLLLGYVSAAESYLRALIRNLINHDEYIQSIVSDKAVSYA
                     AAQHHKKELLPEALLEDFSLASPYNVFETLKDIIGMKGQRPIEMMKCSSEFMKVCELR
                     HCCVHRFGKLGSKNAIRLGLAEHMKHL"
     gene            complement(715..>1118)
                     /locus_tag="L2X49_005030"
     CDS             complement(715..>1118)
                     /locus_tag="L2X49_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBN4799955.1"
                     /translation="KILRKNAKHPVRRNISSLFNAEQLARLKLTLPPCNNCKTPRLTD
                     NQKFCHHCGNQLIDESAYNQCMSIPLSKVPHLTSWQMQKLSGLEKVKTIGDVLSLQDP
                     GSELRKIHRIGPKIANKIMDKVLLHVEEFLS"
BASE COUNT          336 a          215 c          184 g          383 t
ORIGIN      
        1 tctaagtgct tcatatgttc agccaatcct agtcggattg catttttcga acccaactta
       61 ccaaatcgat gaacacaaca atgccttaac tcgcaaactt tcatgaattc gcttgagcat
      121 ttcatcattt cgattggtcg ttgtcctttc attccaataa tgtcctttaa tgtttcaaaa
      181 acattataag ggcttgccaa tgaaaagtcc tcaagtaaag cttctggtaa taactccttt
      241 ttatggtgtt gtgccgctgc ataactaacg gccttatcag aaactataga ctgtatatat
      301 tcatcatgat taattagatt tctgataaga gctcgcaaat aactttcggc tgcagacaca
      361 taacctaata acaacagatg gcttaatgtg tttgaaatat caccagatct taacaagtga
      421 agttgctttg tattagctat aaaaagatct aacggggatt ccttgctatc aacaggtaac
      481 tcattaaatt ttttgtgtgt ctcaaacgaa aaaactagag ctggattact acatagttca
      541 aaaaactctc ttccatctaa gttatcatgg gttgcagtga caactgagga cgccacatta
      601 tgatgagtgg tctcttcata atcctctttt gattctagtt ccattttccc gactcttttt
      661 aaattaaact tttcagataa gaaagacacg attgacaagt acatgccatc aaaattaaga
      721 caaaaactct tcaacatgta ataacacttt gtccataatt ttattggcaa ttttgggtcc
      781 tattctgtga atttttctta actcactccc agggtcttgt aaagacaata catctcctat
      841 agtttttact ttttccagcc cagatagttt ttgcatttgc caagatgtta gatgtggtac
      901 ttttgacagt ggaatagaca tgcactgatt ataggccgac tcatcaatca attgattccc
      961 acaatgatgg caaaattttt gattgtcagt caatcgcgga gtcttgcagt tattgcatgg
     1021 aggtaaagtt agtttaagtc tcgcaagttg ctcagcattg aataatgatg aaatgtttct
     1081 tctgacagga tgcttagcat tctttcttag tatttttt
//
LOCUS       DAFNBJ010000364         1116 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.375, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000364 DAFNBJ010000000
VERSION     DAFNBJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.375"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            9..>1116
                     /locus_tag="L2X49_005031"
     CDS             9..>1116
                     /locus_tag="L2X49_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HBN4799956.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPESENKESQQQQPSTPTDQQVLAA
                     QQAAIKEAEQRSVADKATADAKAKALAQQEAQQYSDKQALQGCLQAAPKYQHAAREKA
                     ASQIANPGTARYKQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAV
                     RVEEIVNYFPYDWDIKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKS
                     EELPASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALP
                     SISGSHKAEINAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNV
                     GIDDPKSIESMVKKQRESGVTLSTFGVGNSNYNEA"
BASE COUNT          321 a          260 c          275 g          260 t
ORIGIN      
        1 gattttatat gcgaaataaa aatataatca tgttgcttat gagtagtttg attttgtcag
       61 gatgcgggcc ggaatctgag aataaggaaa gtcagcaaca acagcccagt acccccacag
      121 accagcaagt gcttgccgcg caacaagctg caataaaaga ggccgagcaa agatccgtcg
      181 ccgataaagc cactgcagac gcgaaagcaa aagccttagc ccagcaagaa gcgcaacaat
      241 attcagacaa acaggctttg caggggtgct tacaggcggc tccaaaatat caacatgcag
      301 ccagagaaaa agcagcgtcc caaatcgcaa atccaggaac cgctcgctac aagcagttcg
      361 atgataatcc ggttaagcag gtagcgcaaa acccgttggc gacgtttagc cttgacgtcg
      421 acaccggcag ttatgcgaat gtacgccgtt ttctcaatca tgggctgtta cctccgccag
      481 acgctgtgcg ggtggaggag atagtcaatt atttcccgta tgactgggat atcaaagaca
      541 aacaatctat tccggccact aagccaatac ctttcgctat gcgctacgaa ctggcacctg
      601 cgccgtggaa tgaacagcta acattgctga aaattgatat cctggcgaaa gatcataaaa
      661 gtgaagagtt accagcttct aatctggtct ttcttatcga cacttctggt tcaatgattt
      721 ctgatgaacg tttgccactt atccagtctt cgttgaaatt attggtcaaa gaacttcgtg
      781 agcaggataa cattgccatc gtgacctacg ctggcgactc ccgtattgcg ttgccttcta
      841 tctccggtag tcacaaggcg gaaattaatg ccgcaattga ttcgctggat gccgaaggca
      901 gtaccaatgg cggtgccggg ctggaactgg cttatcagca ggcggcgaag gggtttatta
      961 agggcggcat caatcgcatt ttattagcca ctgacggtga ctttaacgtt ggcattgacg
     1021 atccaaaatc gattgaatca atggtcaaaa aacagcggga gtctggtgtt actctgtcga
     1081 cgtttggcgt ggggaatagc aattacaacg aggcaa
//
LOCUS       DAFNBJ010000365         1097 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.376, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000365 DAFNBJ010000000
VERSION     DAFNBJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.376"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..1094
                     /locus_tag="L2X49_005032"
     CDS             join(<1..193,193..1094)
                     /locus_tag="L2X49_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HBN4799957.1"
                     /translation="LKLAERIGVTAAARELSLYESQLYNWRSKQQNQQTSSERELEMS
                     TEIARLKRQLAERDEELAILPKGRDILREAPEMKYVFIEKHQAEFSIKAMCRVLRVAR
                     SGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKT
                     VAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGQNQKWAGDITYLRTD
                     EGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQY
                     CSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVF
                     NYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT          268 a          282 c          318 g          229 t
ORIGIN      
        1 ctgaagcttg ctgaacgcat cggtgttact gccgcagccc gtgaactcag cctgtatgaa
       61 tcacaactct acaactggcg cagtaaacag caaaatcagc agacgtcttc tgaacgtgaa
      121 ctggagatgt ctaccgagat tgcacgtctc aaacgccagc tggcagaacg ggatgaagag
      181 ctggctatcc tccaaaaggc cgcgacatac ttcgcgaagc gcctgaaatg aagtatgtct
      241 ttattgaaaa acatcaggct gagttcagca tcaaagcaat gtgccgcgtg ctccgggtgg
      301 cccgcagcgg ctggtatacg tggtgtcagc ggcggacaag gataagcacg cgtcagcagt
      361 tccgccaaca ctgcgacagc gttgtcctcg cggcttttac ccggtcaaaa cagcgttacg
      421 gtgccccacg cctgacggat gaactgcgtg ctcagggtta cccctttaac gtaaaaaccg
      481 tggcggcaag cctgcgccgt cagggactga gggcaaaggc ctcccggaag ttcagcccgg
      541 tcagctaccg cgcacacggc ctgcctgtgt cagaaaatct gttggagcag gatttttacg
      601 ccagtggcca gaaccagaag tgggcaggag acatcacgta cttacgtaca gatgaaggct
      661 ggctgtatct ggcagtggtc attgacctgt ggtcacgtgc cgttattggc tggtcaatgt
      721 cgccacgcat gacggcgcaa ctggcctgcg atgccctgca gatggcgctg tggcggcgta
      781 agaggccccg gaacgttatc gttcacacgg accgtggagg ccagtactgt tcagcagatt
      841 atcaggcgca actgaagcgg cataatctgc gtggaagtat gagcgcaaaa ggttgctgct
      901 acgataatgc ctgcgtggaa agcttctttc attcgctgaa agtggaatgt atccatggag
      961 aacactttat cagccgggaa ataatgcggg caacggtgtt taattatatc gaatgtgatt
     1021 acaatcggtg gcggcggcac agttggtgtg gcggcctcag tccggaacaa tttgaaaaca
     1081 agaacctcgc ttaggcc
//
LOCUS       DAFNBJ010000366         1097 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.377, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000366 DAFNBJ010000000
VERSION     DAFNBJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.377"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..1009
                     /locus_tag="L2X49_005033"
     CDS             <1..1009
                     /locus_tag="L2X49_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBN4799958.1"
                     /translation="GNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTN
                     GILLNNEWCSFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQV
                     EFNTLTVINNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIID
                     FSVPPTAYGKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLV
                     VESNGDIYECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNG
                     GCPKHRITKVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMEN
                     N"
     gene            complement(975..1097)
                     /locus_tag="L2X49_005034"
     CDS             complement(975..1097)
                     /locus_tag="L2X49_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799959.1"
                     /translation="MTTHPTGIYVGCFVKVIRRRLRLELRGRVINYSPFVLQHP"
BASE COUNT          334 a          211 c          216 g          336 t
ORIGIN      
        1 tggcaatcag gtctatttta cctggcaagg cggtgaaccc actctggctg gcctggattt
       61 tttccgtaaa gttattcact atcaacaacg ctatgcaggc caaaaacgta tttttaatgc
      121 attacaaacg aatggcattt tattgaataa tgaatggtgt tcctttctca aagaacatga
      181 atttctggtt ggtatctcga tcgatggccc ccaggagtta catgaccgtt acagacgcag
      241 taattcaggt aacggtactt ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata
      301 tcaagtagag tttaatacgt taaccgtcat taataacgtt aatgtccatt accctcttga
      361 ggtttatcat tttttaaaat ctatcggcag taaacatatg caatttatcg aattgctaga
      421 aaccgggacg ccgaatattg atttcagtgg tcatagtgag aacacattcc gtatcattga
      481 tttttctgtg cctcccacgg cttatggcaa gtttatgtca accattttta tgcaatgggt
      541 taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa agctttgtca gccgtttttt
      601 ggggaatggg catacaagtt gtattttcca agagtcctgc aaggataatc tggttgttga
      661 aagtaatgga gacatttacg aatgcgacca ttttgtctat ccacagtaca aaattggaaa
      721 cattaataaa tctgaactca aaacgatgaa cagtgtacaa ctgacagcgc aaaaaaaacg
      781 gatttcagcg aaatgtcagc aatgtgcata taaacctatc tgcaatggcg gttgtcctaa
      841 gcatcgtatt actaaagtaa acaatgagac tgtttcttat ttttgcgaag gttataaaat
      901 ccttttttca accatggtac cttatatgaa cgccatggta gagttagcta agaacagagt
      961 accgctttat cacattatgg atgttgcaaa acaaatggag aataattaat aacacgccct
     1021 cttagctcca gtcttagcct ccgtcttatg accttaacaa agcacccgac ataaattcct
     1081 gtcgggtgtg tcgtcat
//
LOCUS       DAFNBJ010000367         1081 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.378, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000367 DAFNBJ010000000
VERSION     DAFNBJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.378"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>1081
                     /locus_tag="L2X49_005035"
     rRNA            <1..>1081
                     /locus_tag="L2X49_005035"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          269 a          252 c          336 g          224 t
ORIGIN      
        1 gagtaaagtt aatacctttg ctcattgacg ttacccgcag aagaagcacc ggctaactcc
       61 gtgccagcag ccgcggtaat acggagggtg caagcgttaa tcggaattac tgggcgtaaa
      121 gcgcacgcag gcggtttgtt aagtcagatg tgaaatcccc gggctcaacc tgggaactgc
      181 atctgatact ggcaagcttg agtctcgtag aggggggtag aattccaggt gtagcggtga
      241 aatgcgtaga gatctggagg aataccggtg gcgaaggcgg ccccctggac gaagactgac
      301 gctcaggtgc gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta
      361 aacgatgtcg acttggaggt tgtgcccttg aggcgtggct tccggagcta acgcgttaag
      421 tcgaccgcct ggggagtacg gccgcaaggt taaaactcaa atgaattgac gggggcccgc
      481 acaagcggtg gagcatgtgg tttaattcga tgcaacgcga agaaccttac ctggtcttga
      541 catccacaga actttccaga gatggattgg tgccttcggg aactgtgaga caggtgctgc
      601 atggctgtcg tcagctcgtg ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc
      661 ttatcctttg ttgccagcgg tccggccggg aactcaaagg agactgccag tgataaactg
      721 gaggaaggtg gggatgacgt caagtcatca tggcccttac gaccagggct acacacgtgc
      781 tacaatggcg catacaaaga gaagcgacct cgcgagagca agcggacctc ataaagtgcg
      841 tcgtagtccg gattggagtc tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt
      901 ggatcagaat gccacggtga atacgttccc gggccttgta cacaccgccc gtcacaccat
      961 gggagtgggt tgcaaaagaa gtaggtagct taaccttcgg gagggcgctt accactttgt
     1021 gattcatgac tggggtgaag tcgtaacaag gtaaccgtag gggaacctgc ggttggatca
     1081 c
//
LOCUS       DAFNBJ010000368         1080 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.379, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000368 DAFNBJ010000000
VERSION     DAFNBJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.379"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(388..>1080)
                     /locus_tag="L2X49_005036"
     CDS             complement(388..>1080)
                     /locus_tag="L2X49_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN4799960.1"
                     /translation="VREDRVDYALRQSFLERFAYFTLHAPVSDIPDYIKPFLDGFNGS
                     EPISELFKKFILVEDRLNTYAKFWKVWDLFFDKVVTLCKDGDRYWYVDKIIKSYLFAE
                     SPWKENSNGWHTFKDSNSQFFCDVSRTMGHCPSTLYSLAKSLNNIASCYLNQGITWLS
                     EMLSVNKKLWEKKLENDTVYFLECLVRRYINTERERIRRTKQLKEEVLVILDFLVEKG
                     SVVGYMSRENIL"
BASE COUNT          370 a          211 c          176 g          323 t
ORIGIN      
        1 aaggtcagat cgatatcgaa ctccaaagat tgatacgggc gcggtataac accgcggtag
       61 aaaattggag tgaatggaaa tggttagaaa tcgggcatgg cgtcgtcatc atatatctcg
      121 gttaaaatct aagagaaaaa catatcgaat tgcgttggct aaaactgaaa aagttgtagg
      181 gaaaatttac tgcacccctt gtgcttgttc ttgttattta tgtggtaatc aaagaattta
      241 tcacggagtg aacattcaag agcaacgagc tttgctgcgt tttactgatt gacttataag
      301 atattaatct tttcgataat aaaaaggctc caatccctgg agcctaaatt ggaaaagctc
      361 tcattaaaat tattattttc aactacatca cagaatattt tcccgtgaca tataaccaac
      421 aacagatcct ttctctacca aaaaatccaa tattactaag acctcttctt tcaactgttt
      481 ggttcgtcta attcgctcac gctcagtgtt aatataccgc ctaaccaaac attctaaaaa
      541 ataaacagta tcattttcca atttcttttc ccatagcttt ttattaaccg acaacatttc
      601 tgaaagccag gttatacctt gattaagata gcaactggca atgttattca aagatttggc
      661 aagagaatat aaagttgaag ggcaatggcc catagtccta gatacatcgc aaaagaattg
      721 actattgcta tctttaaatg tgtgccaacc attagagttt tctttccatg gagattcagc
      781 aaaaaggtaa ctttttataa ttttatctac ataccaatac ctatctccat ccttgcacaa
      841 agtaaccact ttatcaaaaa acaaatccca aaccttccaa aatttggcgt aagtatttaa
      901 tctatcttcg acgagaataa attttttaaa taactctgaa ataggctctg aaccgttgaa
      961 accatcaaga aaaggtttta tataatcggg aatatcgctc acgggcgcat gaagcgtaaa
     1021 gtaggcaaat ctttccaaga aagactgccg aagagcgtaa tcaactctat cttctcgaac
//
LOCUS       DAFNBJ010000369         1080 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.380, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000369 DAFNBJ010000000
VERSION     DAFNBJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.380"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..>1080)
                     /locus_tag="L2X49_005037"
     CDS             complement(<1..>1080)
                     /locus_tag="L2X49_005037"
                     /inference="COORDINATES: protein motif:HMM:NF013232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799961.1"
                     /translation="ASVCDALDELSKTLFDIMIIDIQIPDIDGGDINPQGGVELLNNV
                     EHLTHSKIPRYIFGLTSNSSDVSSHFDTFKKFGWPLFDLRNDADCWKDLLVTKARAIE
                     KNINYMSADVAIITALEDTELEELLKLAPSYTSSNIDGYRYYFYEVTTVNGTKLKVVS
                     SSAERMGVTWSSQLATRIIEKFKPRIILMTGICAGVSGKTSLGDIIVGDPVWDWGAGK
                     ISEDHEGNTIFLPDPHQLALNRKVKEQLRDLSQDTVFLKSLVISWPHNTLTSAPQILI
                     APMACGSSVIATQATVDDIADKHRKVTAIEMESYAVMAAAFSYDIPCAVIKSVCDLGN
                     KHKADEIQNYCAYSSASVAIKLIID"
BASE COUNT          334 a          220 c          183 g          343 t
ORIGIN      
        1 tatcaatgat gagttttata gccacggaag cactagaata agcacagtag ttttgaatct
       61 catcagcttt atgcttattc cctaaatcac aaactgattt aataacggca caaggaatat
      121 cataagaaaa ggccgcggcc attactgcat aagactccat ttcaatagct gttactttac
      181 gatgtttatc tgcaatatca tctacagtag cttgagttgc aatgaccgat gatccacaag
      241 ccattggggc tattaatatt tgcggtgccg atgttaaagt gttgtgtggc caagaaataa
      301 ctaaagactt caaaaaaaca gtgtcttgcg ataaatctct tagctgttcc ttcacttttc
      361 tgttgagagc caattgatga gggtctggca agaaaattgt attaccttcg tgatcttcag
      421 atattttacc agctccccaa tcccaaacag gatcgccgac gataatatct cctaacgaag
      481 tttttcctga tacaccggcg caaatccctg tcatcagtat tattcttggt ttgaattttt
      541 caataattct agttgctagt tgtgacgacc atgtaacgcc cattctctct gcagaagatg
      601 aaacaacttt tagtttagtt ccattaactg tagttacttc atagaaataa tacctgtagc
      661 catcaatatt tgaagaagtg tatgatggtg ctagctttaa taattcctct aactctgtat
      721 cttctaacgc cgttatgata gcaacatcag cactcatgta atttatattt ttttcaattg
      781 cacgagcttt tgtgacaagc aagtctttcc aacaatcagc atcgtttctt aagtcaaaca
      841 aaggccaacc aaattttttg aaagtatcaa agtgtgagct tacatccgag gagtttgatg
      901 ttaatccaaa aatatatcgt ggtatttttg aatgagtcag atgttcaaca ttattcaaca
      961 actccacgcc tccttgaggg tttatatcgc ccccatctat atcaggaatc tgaatatcga
     1021 taatcatgat atcaaagagt gtcttactaa gctcatccaa tgcatcacaa acagaagcta
//
LOCUS       DAFNBJ010000370         1073 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.381, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000370 DAFNBJ010000000
VERSION     DAFNBJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.381"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..213)
                     /locus_tag="L2X49_005038"
     CDS             complement(<1..213)
                     /locus_tag="L2X49_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799962.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTC"
     gene            complement(920..>1073)
                     /locus_tag="L2X49_005039"
     CDS             complement(920..>1073)
                     /locus_tag="L2X49_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBN4799963.1"
                     /translation="NPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRR
                     KNKENP"
BASE COUNT          315 a          227 c          182 g          349 t
ORIGIN      
        1 gcatgtatag gattcggaaa gtccagcatc tattttgtac ctcattatat tagataagga
       61 tgtgagaaat tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc
      121 ggccttacag gcaaaatagc ttagtgaaca cgcttttcgt ccctctttag tactggctga
      181 tattagtgcc cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc
      241 agtaactgca ctcgcagaaa tagagaaaaa aaaacaattg ttaataacct tgccatattt
      301 tttagtccgt aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt
      361 gcatgtcatt agataaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg
      421 gaccaaaagc gtttaaacaa ctatgtattg aaaatccatt gcgattataa gttcctgtat
      481 taactgacgg tactaatcgt atgccgtcag gttttctatt gcttgctccc gggtcattaa
      541 cattccaact ccataggcct tgaggtattg gccatctatt tataaatgtg tacaacgggg
      601 attattttta cattgcatat tattaccaga agcgactgga atactaagca catcataaaa
      661 attatcatca catggtatat ctgaaacaca cgaaagcata cctaaagtta tactgtataa
      721 acaggtggct aatcccaagg ggatcgattc cagaaatcgg gttcaacgga tactgataaa
      781 aattccacct accttccagc ccaatcggag cctgagtgtt ataccgcccc tgctgcggtt
      841 aatgttaccg ataatagtta tagtacagcc ctgattcatc atcatactac tgctgcggca
      901 gtctgatgaa ctattgcttt tacggattct ccttattctt ccttctatcc attcgcttta
      961 gctttttgag ctcttgaagt ttgctctttg catcaatatt accgttattt acagcgaatt
     1021 ccatccaata aatagctcta ttcaaattat aatattctga taatgttgga ttt
//
LOCUS       DAFNBJ010000371         1065 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.382, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000371 DAFNBJ010000000
VERSION     DAFNBJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.382"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            74..181
                     /locus_tag="L2X49_005040"
     CDS             74..181
                     /locus_tag="L2X49_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799964.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(234..992)
                     /locus_tag="L2X49_005041"
     CDS             complement(234..992)
                     /locus_tag="L2X49_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBN4799965.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          291 a          261 c          252 g          261 t
ORIGIN      
        1 caaaaaatcc cttgatgcct gtcccttttg ttacactccg ttatcacgca caagagatat
       61 gcaggacact ggtatgccga ctaaacgctt tgataaaaaa cactggaaga tggtggtggt
      121 gctactggca atctgtggcg ctatgttgtt gctacgttgg gcagcaatga tttggggctg
      181 agcagtgaca aacgaagaca gccagaccgc ataacacggt ccggcagata attttacatg
      241 ctgtagccca gcgataaggt tgtccgacga tcggtatgtt ccggtgcaga ctctggaggt
      301 tcagagttcc atgtgacgtt gtaagccact ttcagtccaa aatgttcatt aatagcaaca
      361 tttaatgcgc tttcggagtt cagcgttgtg tcttccgcgc caaagacgga aacaccctgc
      421 gtaaatttag cgttgtcagt caactgccat gcataagcac cggaggcata gcccagcggc
      481 tgagtttcac tggcattgtc ggtatatttg tcgtaacgca cacctggacc gaattcaaag
      541 cggaaactgt gtaccgggcc attcaaaaac tgacgaccat aacccgcggt caacacatcg
      601 cgctctcgat agccgttata acggtctgtc agccagcttg cctgaccaaa taaatagtca
      661 taatcagtta aattaaaacg gctacgcccg cccgccgcat atttttctga agaacgctca
      721 tcattagaag aagtattact ggcgttcccc cacagcgacc aggcagtggt ttgtccatac
      781 caggtcatgg tggtgtcagc cgtaagtgag gagcttttcg tattgcctga ttgtgcaaga
      841 tatcctgcgt tcagattacc ttcgaaaggt tttttagcgc tggcaggatc gtccatgaca
      901 gtaaaaacgg aatcatcggc agctgcattc agtgacgcaa acatgccccc cgccaacata
      961 acgatggcag gaactgtctt caaaagcttc atttatcaag agtccgtaca acaaaaaaag
     1021 agaccatcgc ggtcccggaa actttcttaa ggatcaaaga ttagc
//
LOCUS       DAFNBJ010000372         1064 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.383, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000372 DAFNBJ010000000
VERSION     DAFNBJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.383"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..256
                     /locus_tag="L2X49_005042"
     CDS             <1..256
                     /locus_tag="L2X49_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799966.1"
                     /translation="NIFEHNGRCFMKKNETWMLLNGDATPNIRTMSNKKIECLEKSEV
                     EWASRVCDSITDKERENLKALICSKPAKSVNTGKRPALGY"
     gene            320..874
                     /locus_tag="L2X49_005043"
                     /pseudo
     CDS             320..874
                     /locus_tag="L2X49_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
     gene            901..1041
                     /locus_tag="L2X49_005044"
     CDS             901..1041
                     /locus_tag="L2X49_005044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799967.1"
                     /translation="MSDFDIVSSIDWHDGVLLEVKTTFKESHFNMTLFIDIYKSENIR
                     EK"
BASE COUNT          356 a          170 c          258 g          280 t
ORIGIN      
        1 taatatcttt gaacataatg gaagatgttt tatgaagaaa aatgaaacat ggatgttatt
       61 aaatggagat gcaacaccaa acattagaac aatgagcaat aagaaaattg aatgcctgga
      121 gaagagtgaa gtagaatggg ctagtagggt ttgcgacagt atcacagaca aggaaaggga
      181 aaatctgaag gcattaatat gtagcaaacc agctaaatca gttaatacag gcaaaagacc
      241 agcgttagga tattgaaacc ggcactgcac tatatcacta ttacaatatt ctgagtgact
      301 agagcaactg ctccgccatc cgggctagca gtatgacgac gaatccgaac tgcactacaa
      361 ctggcagcgg tactacaatc cgggacaggg acgatatatc acccaggacc cgatagggct
      421 ggcgggggga ttaaacccgt acagttatgt tatgaacaat cctgtgcaat ggattgatct
      481 attgggatta gctcatactt caggaaaatg gaaggactgt ggtaaaggtt gtcgaatccg
      541 aatagatggc gatgctgttg gtgacggaag acatttgcat tgggagtgta aaaatgggag
      601 tggtgtaatg ggggaatttg gaggtatgtc ccatggtgag gattactcat cagcaccaaa
      661 tagcattaag aaatgtgcaa aaaaatatgg tttcgagcca gaaccaagta aagatctgtc
      721 atgcaaaacc tccgggctat caactactga tgcggctctt gcaactggtg ctacaattgg
      781 cgcagggtat gttatatata gagtgattcg atttatacct tcacttgcac cacctctttg
      841 gtggtcaata cctgcaaacg ttgttactcc atgattaaaa cggataatgg agattatata
      901 atgagcgatt ttgacattgt gagttcaatt gactggcatg atggggtttt gttagaagta
      961 aaaacgacat tcaaagaatc tcattttaac atgacgttat ttattgacat ttataaaagt
     1021 gagaatatta gggaaaaatg atagttgaat tttctgatgt agat
//
LOCUS       DAFNBJ010000373         1033 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.385, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000373 DAFNBJ010000000
VERSION     DAFNBJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.385"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            69..>1033
                     /locus_tag="L2X49_005045"
     CDS             69..>1033
                     /locus_tag="L2X49_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HBN4799968.1"
                     /translation="MKKINFAFIILFLFSLPLIIFYQPWVNALPPMPRHANPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFVSSGARITSQDVPITGGPYKNIVADYGP
                     TDGPLIIIGAHYDSASSYENDELTYTPGADDNASGVAGLLELVRLLHQQVPKTGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTVFYRNKQYHLPGDTADRLNYQKMAQVVDGVINLLYN"
BASE COUNT          290 a          232 c          220 g          291 t
ORIGIN      
        1 aataagatgc aatatcgaaa cccataacaa ataatgattc aataacgaaa cccattaata
       61 atttgcatat gaaaaaaata aattttgctt ttattatatt atttctgttt tcactccctc
      121 ttattatttt ttaccaaccc tgggtcaatg cattaccgcc aatgccccga cacgcaaatc
      181 ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac tgtgcatcca cgtagtgccg
      241 acaatattga taatctgaat aggtccgcag agtatataaa agaggtcttc gtcagtagcg
      301 gtgccagaat tacctcgcag gacgtcccca ttacgggtgg cccctacaaa aacattgttg
      361 ctgattatgg tcctaccgat ggaccgctga ttattattgg tgcgcactat gactctgcca
      421 gcagttatga aaacgatgaa ttgacctata cacctggcgc ggatgataac gccagcggcg
      481 tggcaggatt actcgaactg gtacgtttgt tacatcagca agtaccgaaa acaggcgtgc
      541 agctggtcgc ctatgcgtcg gaagaaccgc ccttctttcg tagcgatgaa atggggagcg
      601 cggtacatgc agcttcgctt gagcgtccag taaaattaat gatagcactg gagatgattg
      661 gctattacga ctctgcgcct ggtagccagg attaccctta cccggcaatg tcctggcttt
      721 atcccgatcg gggagacttt attgccgtgg tcggcagaat gcaggatatc aacgccgttc
      781 gtcaggtaaa agcgacattg ttatcatccc ggtatttatc tgtttattct atgaatgccc
      841 cagggtttat tcccggtatt gatttctctg atcaccttaa ttattggcaa cacgatattc
      901 ccgccgtaat gattactgac accgtttttt atcgtaataa acaataccac ttgcccggtg
      961 ataccgcaga cagattgaat tatcagaaaa tggctcaggt ggtggatggt gttataaatt
     1021 tgttatacaa cag
//
LOCUS       DAFNBJ010000374         1007 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.386, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000374 DAFNBJ010000000
VERSION     DAFNBJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.386"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(405..>1007)
                     /locus_tag="L2X49_005046"
     CDS             complement(405..>1007)
                     /locus_tag="L2X49_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN4799969.1"
                     /translation="YIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCL
                     PQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRC
                     YNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTT
                     VKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          310 a          200 c          181 g          316 t
ORIGIN      
        1 agttcaaata aaagggctca cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc
       61 atcttaacgt ataaaccaga tttaataaca acgaactaga tttaataaca acgatatccg
      121 tacctatccg gatatctaat gtcataagca tccctttaat tatcccctca acctcttgta
      181 actttcccac tcttgaactc cacatagtcc aaacgccgga tgcaaaaaaa tcacacgtgc
      241 atttctgaaa ttgtgatgga ggaagatagt atcaattata tgtgcttata aacgtccccg
      301 attagcgcag aagtcaccgg attgttcagg ctccaatagg ttgagtatgc tgtatttatt
      361 ttccaaaata aaaatcgatg ctgttgctcc ttcataaaat acttttactc tctggacgct
      421 tttcttagct ctcttggtgt tatcttaaaa cgattatgga acctttcagt aaaacgagaa
      481 ggacacttgt aaccattttc cctggcaatc tcgctgatgg gttttactgt cgtttgtata
      541 gcagacaacg cattatttaa cctcacgtcg tccaatatac tttgaaaact tactccctcg
      601 cttgccagac ggcgatgcaa tgtggaaaca gaaatgtaga gatatctcgc taccttgttt
      661 gctgtccatt ttgttccagg ttcggatagc attaggttat aacaacgact tattaatgat
      721 tgtttactat ctgataaaag taaacaatta acatgatgtg ctcctaatga aagtagaacg
      781 cccattgcta agtgttcctg aatttgcgtt gagaaacctc gggaaataga tgtttttagt
      841 tgctcccaac aatatattaa ctcgggattc tgaggtaagc agaaacctgt cgtgttacga
      901 atttgatcgg ttatcgcata aagattttgg aacttttcaa ttagatcaat gggataataa
      961 agcatttctg caagataaag acctgcttct gggtaattcg caatata
//
LOCUS       DAFNBJ010000375          981 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.387, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000375 DAFNBJ010000000
VERSION     DAFNBJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.387"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..271
                     /locus_tag="L2X49_005047"
     CDS             <1..271
                     /locus_tag="L2X49_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HBN4799970.1"
                     /translation="AFVCMAAQEVIEQRESNSDSAPYTLLKNYADRFKKYSAEEQYEI
                     DFQHRLVNRNCYLDKYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            complement(342..>981)
                     /locus_tag="L2X49_005048"
     CDS             complement(342..>981)
                     /locus_tag="L2X49_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HBN4799971.1"
                     /translation="RIADVGNGNYSYIDTLSEAQKVLNSEMRQTLITVAKDVKAQIEF
                     NPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLFELTLNGQKASIDKLR
                     YAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTINAPSEDMRFCAAVAAY
                     GQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFIRLIELADGVTDISQ"
BASE COUNT          268 a          232 c          194 g          287 t
ORIGIN      
        1 tgcgtttgtg tgcatggcag cccaggaagt gattgaacaa agggaaagca attctgacag
       61 cgccccctat acgttattaa aaaactatgc tgacagattt aaaaaatatt cagcagaaga
      121 gcagtacgaa attgattttc aacatcgact ggtaaacaga aattgttatt tagataaata
      181 tggcgatgca cgaatcaatg cttcttatac acaattagaa ataaaacact cacaacccaa
      241 aaatagagca tccggcaaaa gagtgagtta acatcattgt taatcaattc gaccagtcag
      301 caaatatgct gactggtcga acatagtttg ctaaacagtc atcactgact gatgtcagtc
      361 acaccatccg ccagttcaat caggcgaata aattccgccc tgtaaccctg tggatcttcc
      421 ccttttgcct gctgagccca ctgtttgatc tgctgccagg aggtattgtt caggtattca
      481 gaaccgcgta acttttgccc atatgcagct actgctgcgc aaaaacgcat atcttcagag
      541 ggggcgttta ttgttggccc cagcgggaat tcaactaact gactttcttt tccctgcggg
      601 tatttccagc gaatctttaa ccaggccagt tctttcgttt tgtccgattt cactgatttg
      661 ttatccgggg catagcgtaa cttatcaatt gatgcttttt gcccgttcag cgttaattcg
      721 aataacaacg ttatatgttt gcctgcgcca atatcccccg catcaacgtt gtcgttatta
      781 aaatcttccg cccgaagttg gcgcttttca taaccaatct gacggtattc cgttacccac
      841 gcggggttaa actcaatttg cgctttgaca tcttttgcta cggtaatcaa cgtctgccgc
      901 atttcactat tcaatacttt ctgcgcttca gagagggtat caatgtagct gtagttgccg
      961 ttaccaacat cggcaattcg c
//
LOCUS       DAFNBJ010000376          973 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.388, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000376 DAFNBJ010000000
VERSION     DAFNBJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.388"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..927
                     /locus_tag="L2X49_005049"
     CDS             <1..927
                     /locus_tag="L2X49_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN4799972.1"
                     /translation="GEQKIFLILETILKADKNALILIDELDLLLHDEALKKLIDVIST
                     HAEDKNKQIIFTTHREMVTTLSDKINIRHVVNIQGRSYSFEETKPDAINRLTGKSTTP
                     IEIYVEDDLAVAIINKICSSLKASRYVKIFKFGAASNAFTLLASTLIRGDNLSDKLYI
                     LDGDKYSTENEKKAALDKVFTGTESRTYELKAAAEGKIKQFNLPNGVKPEQYIHYLIT
                     NVPLDGLGGEYLEIIEAARDIRVELDAHNYISNILTKLGIDRPSGLTRVMDLASRHPE
                     WHQYVSEVTDWLQPVVSDLMERLPENDTVDIT"
BASE COUNT          362 a          170 c          178 g          263 t
ORIGIN      
        1 ggagagcaaa agatattttt aattctggaa acaatattaa aagcggacaa gaacgcctta
       61 atattaatcg atgaattaga tttgctgttg catgacgaag cattgaaaaa gttaatagac
      121 gtcatatcaa ctcatgccga agataaaaat aaacaaatca tcttcactac ccatagagaa
      181 atggtaacca ctttatcaga caagataaat ataagacacg tcgtaaacat tcaaggccgt
      241 agttattcat ttgaagaaac aaaacctgat gctataaaca gattaacagg taaatcaaca
      301 actcccattg aaatatatgt ggaagatgat cttgctgttg caataataaa taaaatatgt
      361 tcttcactta aggccagcag atatgtgaaa atatttaaat ttggcgcagc ttcaaacgca
      421 ttcactctac ttgccagtac acttattcgt ggagataatc tatctgataa actttatatt
      481 cttgatggcg ataaatactc cacagaaaat gaaaagaaag ctgctcttga taaagttttc
      541 accggcacag aatcacgaac ttatgagtta aaagcggcag cggaaggaaa aataaaacaa
      601 tttaatctcc caaatggagt taagcctgaa caatacattc attatcttat tacaaatgtc
      661 ccactggacg gattgggtgg agaatattta gaaataatag aagcagcaag ggatatcaga
      721 gttgaacttg atgctcataa ttatatctcg aacattttaa ctaaattggg cattgatcgt
      781 ccgtccgggt taacacgagt gatggatctg gcctccaggc atcccgaatg gcatcagtat
      841 gtaagtgaag tcactgactg gttacagccc gtagtttctg atttaatgga acgattacct
      901 gagaatgata cagtagatat aacttaaaat atccaaccat cccgaatgag caaatacaaa
      961 agctcatttg gga
//
LOCUS       DAFNBJ010000377          969 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.389, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000377 DAFNBJ010000000
VERSION     DAFNBJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.389"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            196..798
                     /gene="fimB"
                     /locus_tag="L2X49_005050"
     CDS             196..798
                     /gene="fimB"
                     /locus_tag="L2X49_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HBN4799973.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          297 a          174 c          200 g          298 t
ORIGIN      
        1 agagcatagc gtttgtatgg caacgttatt ataagtaact gttgctactc catttaagtt
       61 cactcagaag aactggtcca cttacgttag ttattaagca aacgttcgct tttataaaca
      121 taatcaggat aaaaatgttg gattattgct aacccagcac agctagtgcg cgtctgtaat
      181 tataagggaa aaacgatgaa gaataaggct gataacaaaa aaaggaactt cctgacccat
      241 agtgaaatcg aatcactcct taaagcagca aataccgggc ctcatgcagc acgtaattat
      301 tgtctgactt tgctttgttt tattcatggt ttccgggcga gtgaaatttg tcgattgagg
      361 atttcggata ttgatcttaa ggcaaagtgt atatatatcc atcgattaaa aaaaggcttt
      421 tcaacaacgc acccgctatt gaacaaagaa gttcaggctt taaaaaactg gttgagtatc
      481 cgtacttcgt acccgcatgc tgagagcgaa tgggtatttt tatcgcgtaa gggaaatccg
      541 ctttctcggc aacagtttta ccatattatc tcgacttccg gtggtaatgc cgggttgtca
      601 ctggagattc atccgcacat gttacgccat tcgtgtggtt ttgctttggc gaatatggga
      661 atagatacgc gacttatcca ggattatctt gggcatcgca atattcgtca tactgtctgg
      721 tataccgcca gcaatgcagg gcgtttttac ggcatctggg atagagccag aggacgacag
      781 cgtcacgctg ttttatagag atagttgaga taccagggat ggtgttaaaa actacattat
      841 tcttacgata tatcaaaaat gattaaccat ttattaattg atgttattgt aagtgtgatt
      901 attaattttc ataataaaca ttaagttaac catatccata caaaatacaa tggtttatgt
      961 tcttaaaaa
//
LOCUS       DAFNBJ010000378          955 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.390, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000378 DAFNBJ010000000
VERSION     DAFNBJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.390"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..258
                     /locus_tag="L2X49_005051"
     CDS             <1..258
                     /locus_tag="L2X49_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799974.1"
                     /translation="QPILGMFLIGIGLWGFYAPDEGTCTNLNIELCAVSAIDVLKPAI
                     FVFLGIITVAGWYFTELQPKLRGEGKYMKVKTGVKPEDFVK"
     gene            294..578
                     /locus_tag="L2X49_005052"
     CDS             294..578
                     /locus_tag="L2X49_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799975.1"
                     /translation="MNKSWLEQQVQNEKLKKVQEEKKDGTIQKGLYRVRIKDKYRWDL
                     WENDFRVVIAYSKDDAELKFTEMTGFNEHYYIESIEKIVLSDIEKILLEN"
     gene            complement(599..>955)
                     /locus_tag="L2X49_005053"
     CDS             complement(599..>955)
                     /locus_tag="L2X49_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="HBN4799976.1"
                     /translation="VMKYQVKEFINEKYSKAVNILKDNLKEHYHIFYGLRLSEILFPA
                     SEYGSEMFFQEFEAINSVILPLVIFDLIDRKPIMVIGFGEVCGVDSLVDSGIEVVSLD
                     GLSDLLLVEKLTPLFN"
BASE COUNT          345 a          154 c          187 g          269 t
ORIGIN      
        1 caaccgatat tggggatgtt ccttataggt ataggcttat gggggttcta tgcacctgat
       61 gagggaactt gtactaatct caatattgag ttatgcgctg tatcagctat tgatgttctg
      121 aagcctgcta tttttgtatt tttaggcatt attaccgttg ccggatggta ctttaccgaa
      181 cttcagccga agctaagggg ggagggcaag tatatgaagg taaaaacagg tgtaaaaccg
      241 gaagatttcg ttaagtaaaa accgagccta aagacgttaa ccgtggggga tttatgaata
      301 aatcatggct tgaacaacag gtgcaaaatg aaaaattaaa aaaagtacag gaagagaaaa
      361 aagatggcac tatccaaaaa ggattgtaca gggttcgtat aaaagacaaa tacaggtggg
      421 atttgtggga aaatgatttc agagttgtta ttgcttattc gaaagatgat gcagagctaa
      481 aatttacaga aatgacaggt tttaatgagc attactatat agaaagcatt gaaaaaatag
      541 tgctttcaga tatagaaaaa atcttactgg aaaactgaaa agatagttat ccagaggttt
      601 agttaaataa tggcgtcagt ttttcaacca gtagcagatc gcttaaaccg tctaaagaaa
      661 caacttctat accagaatca acaagtgaat caactccaca tacttcaccg aagccgataa
      721 ccataatcgg ctttcggtct atcaaatcaa aaatcaccaa aggcaatatt acactgttaa
      781 tcgcttcaaa ctcctgaaaa aacatttcag agccgtattc gctggcagga aaaagaatct
      841 cacttaatct caaaccataa aaaatatggt agtgttcttt aaggttatcc tttaaaatat
      901 taacagcctt agaatatttt tcgttaataa actctttaac ctgatatttc ataac
//
LOCUS       DAFNBJ010000379          949 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.391, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000379 DAFNBJ010000000
VERSION     DAFNBJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.391"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..642)
                     /locus_tag="L2X49_005054"
     CDS             complement(<1..642)
                     /locus_tag="L2X49_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799977.1"
                     /translation="MKSDLTIKNSYCTIPQTKFRKWDEMDVLLWKLGKNDSRRRSGVY
                     YLNAYKDAYVQYNRDKIIKHAYAAGIRPELLGGVAWIESGGMPENYKFQIYETKRMIG
                     LLDMPENKTSFGSMGIQIRTAAITLGLDPSELTTRNQLELATCLMEDDFTFQIAATHL
                     RDLALFDYPSSATLYMTNEQYIMAGIRYNRGVERYLGFFIYLINNLPARDTDDY"
     gene            complement(645..>949)
                     /gene="hcp"
                     /locus_tag="L2X49_005055"
     CDS             complement(645..>949)
                     /gene="hcp"
                     /locus_tag="L2X49_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HBN4799978.1"
                     /translation="KFYKPIDKSSPLLNAALSENEELSAIFNFYRINSGGCIERFYTI
                     ELQGAHLSNILTCYPHSITHAGNQPEEVIVLNYKNITWKHHIASTESYSCWEEMVF"
BASE COUNT          307 a          187 c          142 g          313 t
ORIGIN      
        1 ataatcatct gtatcacgcg caggcaaatt attaattaaa taaataaaaa accccaaata
       61 tcgttcaaca ccacgattat atctaatacc agccataata tactgctcat tcgtcatata
      121 cagtgttgcc gaagagggat aatcaaaaag ggcaagatct cttaaatgag tagcagcaat
      181 ttgaaacgta aaatcatctt ccattaggca tgtcgctaac tctaactgat ttctggtagt
      241 taattctgaa gggtcaagac ctagagtaat cgctgcggtt cgtatttgta tacccattga
      301 gccaaatgat gttttatttt caggcatatc taacaatcca atcattcttt ttgtttcata
      361 tatctgaaac ttataatttt caggcatacc tcctgactca atccaggcca caccgcccag
      421 taactctgga cgaatcccag cggcatatgc atgtttaatt attttgtccc tattatattg
      481 tacatatgca tctttataag catttaaata atatactccc gaacggcgac gcgaatcatt
      541 ttttcctaac ttccagagta aaacgtccat ttcatcccat ttacggaact tagtttgtgg
      601 aattgtacaa tagctatttt ttatagtgag atcgcttttc atatttaaaa aaccatttct
      661 tcccaacaac tatatgattc cgtactcgcg atatggtgct tccatgttat atttttataa
      721 ttaagaacaa taacctcttc cggttgatta cccgcatgtg taattgagtg tggataacaa
      781 gtcagaatat tcgataaatg tgccccttgc aattcaattg tgtaaaacct ttcaatacaa
      841 ccgccagaat taattctata aaaattaaat atagctgata actcttcatt ttcagaaaga
      901 gcagcattta ataaagggga ggatttatct attggcttgt aaaatttta
//
LOCUS       DAFNBJ010000380          942 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.393, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000380 DAFNBJ010000000
VERSION     DAFNBJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.393"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..907)
                     /locus_tag="L2X49_005056"
     CDS             complement(<1..907)
                     /locus_tag="L2X49_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBN4799979.1"
                     /translation="METGKIALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLF
                     FAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASILILKSDLRHKTTLYTINFM
                     LSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLYCFITIALLAGVFSFKDT
                     LLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEK
                     EPRLSGALPYVDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILL
                     FSLLVVGLSDVILFSKPVPTAVFITI"
BASE COUNT          325 a          158 c          171 g          288 t
ORIGIN      
        1 ttatggtgat aaacacagca gtcggaaccg gtttagaaaa gagtattaca tcacttagtc
       61 ctactaccag tagtgaaaaa agcaagatta aaatataagg ttctttagca gttcttaagg
      121 catatatgag tattgctgaa aatgctaaaa ttgttaatac aactccagga ataccacgcg
      181 ttgataacgt atctatgaga tcgttatgca aatgagaatc tacatagggt aaagcgccac
      241 tcaatctagg ctctttttct tctagttcat gtattttttc cgcacgtttt tccagtgatt
      301 gtcctattgg agaatatgtt tttaagccaa cttcatacat tgccagacgg gcaccgactg
      361 aagttctggt gttatcatgc gaataattaa ctaaatcgtt atttaagtca ttcattctca
      421 taagcaatgt atctttaaaa gaaaatacac ctgctaataa tgcaatcgta ataaaacaat
      481 ataacttata attaattcgc ttattaatat aactatccat tacgattaat attaacgcaa
      541 agaatggaaa cactaatatg gctgcacgcg tctcagttac tatgacagca cataaactaa
      601 gcatgaagtt aattgtatat aatgttgttt tatgtcttaa gtcggatttt aaaatgagaa
      661 ttgatgccag taaagaagga aaaaggatga tgtagcctgc tgttgtagcg tgggaagctg
      721 ataatgcaac acggtcgacg tttaaataga aatgttgcca catggcatgc gcaaaaaaaa
      781 gtaactgcgt tgccagggat gcgaacagaa ttagtttccc aaattttatt tttgttctca
      841 tctcattttt agcaaaaaga actaagaaaa taaaagcgct acacaaggca atttttccag
      901 tttccattgg gccacgatat gcattggtat aaacagagcc tg
//
LOCUS       DAFNBJ010000381          936 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.394, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000381 DAFNBJ010000000
VERSION     DAFNBJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.394"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            93..878
                     /locus_tag="L2X49_005057"
     CDS             93..878
                     /locus_tag="L2X49_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBN4799980.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          306 a          185 c          200 g          245 t
ORIGIN      
        1 tgtttgttat tatttaagtg aggtatataa ttagagtccg ttatcaatgc taaatattct
       61 aatcattatg acaggcgagg gagtgtccaa ttatgaattc aaaaaagctt tgttgcatat
      121 gtgtgttatt ctcgctgctt gcaggatgtg cctctgaatc ttctattgat gaaaagaaga
      181 aaaaagctca agtcacacaa agtaatatta ataaaaacac tccccagcaa ctgacagaca
      241 aagatttatt cggtaatgaa accactctgg ccgtatccga agaggatatt caagctgcgc
      301 ttgatggaga tgagttccgc gttcccctta attctcctgt aatacttgtt caatccggca
      361 accgcgcacc ggaaaccatt atgcaggagg agatgcgaaa atattatact gtttccacat
      421 tctctggtat cccggacagg caaaagcctc tgacttgtaa caaaaacaaa gataaaaatg
      481 agaacgagga tgttgccagt gctgagaata tgaactggat gcaggcactg cgttttgtgg
      541 ctgcaaaagg acatcagaaa gcgattattg tttaccagga tacattgcag acaggaaaat
      601 atgactctgc gctgaaatca acagtatggt ccgactataa aaatgaaaaa ctcaccgacg
      661 ctatctccct gcgctacctg gtacgtttca cgctggtgga tgtggcaaca ggtgagtggg
      721 ctacctggtc gccggtgaat tatgaatata aagtgcttcc accattgccc gacaagaatg
      781 aagccagtac gactgatatg acagagcagc aaatcatgca acttaagcaa aagacctata
      841 aagcgatggt aaaagatttg gttaaccgct atcaataata aattatatct gccgccagga
      901 atttctggcg gcaataatac aaaattcttt gcataa
//
LOCUS       DAFNBJ010000382          924 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.397, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000382 DAFNBJ010000000
VERSION     DAFNBJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.397"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..451
                     /locus_tag="L2X49_005058"
     CDS             <1..451
                     /locus_tag="L2X49_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HBN4799981.1"
                     /translation="KDREKKYRDGDKINGPQAHSLDSTNERRFADNRTMFTQHIEKQR
                     NVNTLNQNDINNSVNNANVHENELTYQFQRWGQNHTVRILESSEGIRLKPSDTLVSNR
                     LREAQHNDVTAQRWVLTEQDERQGQRHQPYEEQENESKFENDQKDES"
     gene            451..924
                     /locus_tag="L2X49_005059"
                     /pseudo
     CDS             451..924
                     /locus_tag="L2X49_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT          333 a          158 c          203 g          230 t
ORIGIN      
        1 taaagatcgt gaaaaaaaat accgcgatgg agacaaaata aatggaccgc aggcccattc
       61 attagacagt actaacgaaa gaaggtttgc tgataacaga accatgttta ctcaacatat
      121 cgaaaagcaa aggaatgtta atacgctcaa tcagaatgat ataaataatt ctgttaataa
      181 tgccaatgtg cacgaaaatg aattgacata tcagtttcag cgttggggac aaaatcatac
      241 agtccggatt ctggaaagta gtgaagggat acgactaaaa ccatcagaca cacttgtctc
      301 caacagactc cgtgaagccc aacacaatga tgttacagca caacgttggg tattgactga
      361 gcaagatgaa cgacaaggac aacgacatca gccgtatgag gagcaagaaa atgaaagcaa
      421 attcgaaaac gatcagaagg atgaaagtta atgttcggtc tgaggaaagt aaatcgaaac
      481 actcatatgt ttgaaaccac ttttcaaaat tggaaagaaa atggcgaaga tgttgcatta
      541 ttaatgcccg aatttagtgc taaatggcta ccgatagcag aagagagtgg cagctggtct
      601 ggatgggtat tgcttggaga aatatttccc ttaatttcat ctgagctcgc tgggatggct
      661 ttaatgccag aaacggaaag gttaataggg taatggctca gtttatcaag ttccccgtta
      721 aatctaaagc atcctgaact aaaatataat cgtttgcgcg taggtaaagt atttgatgga
      781 gtattgagcc ctgctcaacc actaataaga atatggacag gggaattgaa tctttggtta
      841 gataaagtca cagtctgtca atacggaaac gctccagcgt tagacaaaaa atcattatat
      901 tggtccattc attttgtaat tgga
//
LOCUS       DAFNBJ010000383          882 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.402, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000383 DAFNBJ010000000
VERSION     DAFNBJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.402"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..323
                     /locus_tag="L2X49_005060"
     CDS             <1..323
                     /locus_tag="L2X49_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBN4799982.1"
                     /translation="RQKLFRFLDNKFDSEKYRNNVRELTPAILAVLPLEYRGHLVEQD
                     SFMARLAEMEKELCEAKQAVILNAPRHQKLKEMSEGIVSMFRVDPDLAGPLMAMVTTM
                     LGAI"
     gene            320..>882
                     /locus_tag="L2X49_005061"
     CDS             320..>882
                     /locus_tag="L2X49_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBN4799983.1"
                     /translation="MTGSKMAKVGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     VAQKERRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTK"
BASE COUNT          258 a          184 c          236 g          204 t
ORIGIN      
        1 cacgccagaa gctgttccgc ttcctcgata acaaattcga ttctgaaaaa taccgaaata
       61 acgtgcgtga actgaccccg gcaattctgg cggtactacc gctggaatat cgcggccacc
      121 tggttgagca ggatagcttc atggctcggc tggctgaaat ggaaaaggaa ctctgtgagg
      181 caaagcaggc ggtcattctc aacgcaccac gccaccagaa actgaaggag atgagtgaag
      241 gcattgtgtc gatgtttcga gtggacccgg atctggctgg tccattgatg gcgatggtca
      301 ccaccatgct gggggcaata tgacaggttc aaaaatggcg aaagtcggtc tgcgggaaca
      361 gaaccgactt tcaggtgcaa atcgtaacac actcattgcg ggaggaatta tggcaaacac
      421 tgctgagata ttcaattttc ctgtgccgga tgtggcacaa aaggagcggc gcgtggcaga
      481 tctcgatgat ggttatacgc gcattgcaaa tgagttgctg gaagctgtga tgctggccgg
      541 attaacacag caccagcttc tggtctttct ggctgtcatg cgcaaaacat atggctttaa
      601 taaaaaactg gattgggtta gcaacgagca actttccgaa ttaaccggga tattgccgca
      661 caagtgttct gctgcaaaaa gtgttctggt aaagcgtggg atttttattc agagcgggcg
      721 gaatatcggc atcaataatg tggtcagtga atggtcaaca ttacccgaat cgggtaagaa
      781 aaataaagtt tacctgaaag aggtaaattt acctgaatca ggtaagaaaa gtttacctaa
      841 atcaggtaaa ggcgtttacc cgaatcaggt aaacacaaaa ga
//
LOCUS       DAFNBJ010000384          880 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.403, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000384 DAFNBJ010000000
VERSION     DAFNBJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.403"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            96..428
                     /locus_tag="L2X49_005062"
     CDS             96..428
                     /locus_tag="L2X49_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799984.1"
                     /translation="MESEQLITKITQTLKRPDGSEVRIVVEQAFGSGLTPSLGVYVLR
                     RPTTADNWQLCKTAPHKDWRTMSVDEYQKHGRSEMLRYVSIGEILRLSAAIGKPMSYV
                     DTCPGLQG"
     gene            485..748
                     /locus_tag="L2X49_005063"
     CDS             485..748
                     /locus_tag="L2X49_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799985.1"
                     /translation="MMINEQGIAVNTPDLSRLYIISGRMMFDDEEEVMLVEADSCGEA
                     EAAFQQHIDEGIAHLDQVLVISSASLQSLLPDRLIASSLTHTV"
BASE COUNT          237 a          211 c          206 g          226 t
ORIGIN      
        1 atgaatgaaa aaaatcactt cttacttatt agccagagag ccgactttgg taaaatatcc
       61 ctacagcaac ccaactgacg gtcgaggtta atagtatgga atccgaacag ctcattacta
      121 aaattactca gacactgaaa cgccccgacg gctctgaagt acgtattgtg gtggagcagg
      181 cattcggcag tggcctcacg ccttcgctgg gtgtatatgt cctgcgtcgc ccgaccactg
      241 ccgacaactg gcagttgtgc aaaaccgcac cgcacaagga ctggcgcact atgtcggtgg
      301 atgaatacca gaaacacggg cgttcggaaa tgcttcggta cgtctccatc ggcgaaatcc
      361 ttcgcctgag cgccgcgatc ggcaaaccaa tgagctacgt tgatacctgt ccgggtttac
      421 agggctgatg ctgtttttta cacgtagcag aactgacaaa tttaaaaatt gaaggcggag
      481 aaaaatgatg attaacgaac agggcatcgc tgtaaatacc ccggacttaa gccgactgta
      541 tattatctca ggccgcatga tgtttgatga tgaggaagag gtgatgctgg tcgaagcgga
      601 ctcctgcggt gaagctgaag cggcattcca gcaacatatc gacgaaggta tagcgcactt
      661 ggatcaggtg cttgttattt cgtctgcgtc cctacaatca ctgttaccag acaggctcat
      721 tgcttcctcc ctgacacata ccgtataacc aaatctttcc cgtctaataa attccgccct
      781 tgtggcggtt ttttgttttc ccgtatattt attttacacc gtatccagaa taaaagttgg
      841 attattaaat atggcgctgt ctgctctata aaaatttcga
//
LOCUS       DAFNBJ010000385          879 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.404, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000385 DAFNBJ010000000
VERSION     DAFNBJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.404"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..>879)
                     /locus_tag="L2X49_005064"
     CDS             complement(<1..>879)
                     /locus_tag="L2X49_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBN4799986.1"
                     /translation="ILLSAYEIACDIEDSEYLDLSTILIADKEFLINLIVRLEQGLSP
                     SLEQENIVQLISVSSVISSLSSLCDWLERKRVIILLDDAAISLTPEYLYEFFDIVRSL
                     KTSKIALKASVYPGTTEYGPRFHVAHDAETIDAWISVQHPNYSSVMDAIAQARFPGYD
                     GIPDDLKELFKFSAFGIPRSFLMMLRDYQTTLSQTTQQKFNKIIERYVELRLSEYSSL
                     KDKLPRLFDIVSLGESLLLKVVELLKESNSQYKEEKQVIIGIEKNDNVYFSRLTKLLI
                     ETGLFYNLPDISHGDG"
BASE COUNT          295 a          163 c          142 g          279 t
ORIGIN      
        1 gcccatcacc atgagaaata tcaggaagat tataaaaaag ccctgtttca attaatagct
       61 tagtgagtcg agaaaaataa acattatcat ttttctctat accgattatg acttgtttct
      121 cttctttata ttgggagtta ctttccttga gtaattcgac tacttttaat aaaagtgatt
      181 cacctaatga aacaatgtca aacaatcgag gtagcttatc ttttagtgaa ctgtattcgc
      241 tgagtctaag ctcaacatac ctttcaatta ttttattaaa tttttgctga gttgtctgac
      301 ttaaagttgt ttgataatct cttaacatca tcaaaaaaga acgtggaata ccaaatgctg
      361 agaatttaaa taactctttc aagtcatccg gaatgccatc atacccagga aaccttgcct
      421 gcgctatagc atccatcact gaggaataat tagggtgctg taccgaaatc caagcatcaa
      481 ttgtttcagc atcatgagca acatgaaacc ttgggccata ttcggttgtt ccaggataaa
      541 ccgatgcctt taaagctatt tttgaggttt ttagactacg tacaatatca aaaaactcgt
      601 aaagatattc aggagttaag cttattgctg catcgtctaa taaaataatg actcttttac
      661 gttcaagcca atcacatagt gaactcaacg aggatataac agaagaaaca ctgatcaatt
      721 gaacaatgtt ttcttgttct aatgatggcg aaagaccctg ctcaagcctt acgattaaat
      781 taattaggaa ctctttatcc gctatgagta tcgttgacaa atcaagatat tcagaatctt
      841 caatatcgca agcaatttca taagcagaga gtagtatct
//
LOCUS       DAFNBJ010000386          878 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.405, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000386 DAFNBJ010000000
VERSION     DAFNBJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.405"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(204..656)
                     /locus_tag="L2X49_005065"
                     /pseudo
     CDS             complement(204..656)
                     /locus_tag="L2X49_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          248 a          211 c          174 g          245 t
ORIGIN      
        1 cttatttatc aattggttat tttggcgcat agcggtcatc aagggaatat cgcgttgtca
       61 taaggtgtcg aggctcggag gttcaaatcc tctcatgcaa aaaataaata aaattaatga
      121 cggttggaaa ttattcaata catacactct cgaaagtgca tcagccaacc gcagcacgtc
      181 ttgcatacgg cgtgtctgca gttttatata atcctggctg gaaacctctt atacaaagta
      241 gatacaccaa tatcatagat gatcgccacc ttctgacgcg ggacttttga tgcaattaat
      301 cgcccggcct gcgcccattg ttctgatgta agtttaggac gacgtccacc aattcctccc
      361 tgtgcgcgag catcttccag tccaactttt gtcagtcatc aatcagttca cgcttcattt
      421 caaccagggc tccatcacat ggaaaaataa aaaaaaccat cgatgttgac gtgtcaatgt
      481 tacctgtcag gctacggaaa tccccccctt acccgcagct actcggtaag tataataaga
      541 tgtttcctgc tccttcctaa cctgtccagt ttccagacaa ttaaagtatc accttatata
      601 agagactgca gagcgcactt taacctaggt cgttctgaaa tagttgcaga ttcgattaat
      661 caacgattat actccccggc actccagagg atctggtaaa cataaagtca gagcttgggt
      721 atactggcac acaaatggca gatcttgcag gtgcagccag tcatagccag tggcgaaaat
      781 acacgagtgg ttctgagccc cgcgccatgt catcacatat cttgtttttt tattgctacc
      841 aatctgactt tgagtactaa tgagctagat agaattgt
//
LOCUS       DAFNBJ010000387          817 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.407, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000387 DAFNBJ010000000
VERSION     DAFNBJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.407"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..497)
                     /locus_tag="L2X49_005066"
     CDS             complement(<1..497)
                     /locus_tag="L2X49_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799987.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCWQ"
     gene            complement(753..>817)
                     /locus_tag="L2X49_005067"
     CDS             complement(753..>817)
                     /locus_tag="L2X49_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBN4799988.1"
                     /translation="INVNFFSSDRTSFCECNRFP"
BASE COUNT          237 a          156 c          161 g          263 t
ORIGIN      
        1 atttgccaac aatctgaagt aatgctggtt ttattaaagt gcgcaggagg tgttaagatg
       61 gcttcttttc gggtgaattt tgcacaatac ttagaaatcg tctccttaat acattccagc
      121 tcttttttat tgacggctga gcaagtacat tgttcaccaa aaggtgcaaa agtgcctaca
      181 tctaatgttt gtatgatata atcgtcatgt tctcctgtag aaatatctat gatgaatgga
      241 ctcatatttt ccggttggat tcggatcatg acagaaggaa catcgccttg atagaaattc
      301 aatgcagtaa ttgcagataa tactctccgc agttcattac ttatactgta aattttatta
      361 acgtcattgg tggagacaat ttctgcaaac tttcttttta ccgtccaata atcaatattt
      421 ctattgtgat tgttaaccac tgcgtgcatt ggcgggattg aagggtgaaa aatatgtgca
      481 attgggaagc ctttcattga tgcctgcctt gtcgtcatga caaacaataa tagatgctta
      541 ttttcctcgc agggttcttt cgcactatca aaaaacgctc atttacccgg cgttacagta
      601 aacgaagcaa cattctgtgg cctacgacga tcaaaagctc tgtgaaaatt tcccttgttg
      661 tctttgtctg accttatcgc ggcgaccagc aattattcgt tttgtgatcc tcaacacaaa
      721 atgctattcg tcgcctatga ggggagaaga atttatggaa aacgattgca ttcacaaaat
      781 gacgtcctgt cagaagagaa aaagttgacg ttaatca
//
LOCUS       DAFNBJ010000388          812 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.408, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000388 DAFNBJ010000000
VERSION     DAFNBJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.408"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            107..649
                     /locus_tag="L2X49_005068"
     CDS             107..649
                     /locus_tag="L2X49_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBN4799989.1"
                     /translation="MKKLSVSELAKLYGYSRQAIYAHINKGNLSKGSDGLIDFSEALR
                     VFGEPQKKEDTVNQSQSINSQNLTEVDLLKRQVDILEKQLNQAIQRENQSLERESFYQ
                     EQIEAMQRLLEAPKANMTTFTDQSFKQDIATDPRSELATNYDELTTPQQDNKRIPIPE
                     HVEPEQKKRGFLSRFFLPYG"
BASE COUNT          304 a          148 c          149 g          211 t
ORIGIN      
        1 ggaattactt cctttactca gaaaattagg atttcaatga cattgttaat tgaaattgtt
       61 aagttaacag tcaggtatta acacaagttg aaaactggtt tacataatga aaaaactgtc
      121 agtttcagag ttagctaagc tttatggata ttcaagacaa gccatatatg cacatataaa
      181 caaaggaaat ttatctaaag gatctgatgg attaattgac ttttcagagg ccctaagagt
      241 ttttggggaa ccacaaaaaa aagaggatac agtcaatcaa agtcaatcaa ttaacagtca
      301 aaacttgaca gaagttgact tactaaaacg tcaagttgac atactggaaa aacaattaaa
      361 tcaggcaata cagagagaaa atcaatcttt agaacgtgaa tcgttttatc aagaacagat
      421 tgaagctatg cagcgcttac tagaagcacc aaaagccaat atgactacct ttaccgatca
      481 gagctttaaa caggatatag caacggatcc tcggtcggaa ctagcaacga actatgacga
      541 attgactact cctcagcaag acaataagcg tattcctatc ccggagcatg ttgaaccaga
      601 acagaagaag agaggcttcc taagccgctt tttccttcca tatggttagc caagactcca
      661 gttttttgaa gagccaattt cagtgcaagc acttgaaata atgactcctc aaaaaaccgg
      721 atgatgttta ttaaaactga ataaaaccgt ggaacatcgc tcaattagtg atcgtggaac
      781 atgaataatc aataaaaagc cgttctggtg cc
//
LOCUS       DAFNBJ010000389          799 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.409, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000389 DAFNBJ010000000
VERSION     DAFNBJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.409"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            109..357
                     /locus_tag="L2X49_005069"
                     /pseudo
     CDS             109..357
                     /locus_tag="L2X49_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611920.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            422..>799
                     /locus_tag="L2X49_005070"
     CDS             422..>799
                     /locus_tag="L2X49_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBN4799990.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLD"
BASE COUNT          237 a          173 c          199 g          190 t
ORIGIN      
        1 tcatcacgta tttatatatc atgaagctat tgttgtgttt tgtactgaat cgccacgggt
       61 ttaacagaca cctcagagtc atttaagatg gcttaaagag aagtgcccat gagcggtaag
      121 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      181 gttgccagcg ttgcaacacg tctcgatatc accacccaca gcctttatgc ctggataaag
      241 aagtacggtc cggattcttc cactaataaa gaacagtcag atgctccggc cgagatccgc
      301 cgtctccaga aagagctgaa acgggttacc gacgaacggg acatattaaa aaaagccgct
      361 gtagattcaa ttggtcaacg caacagttat gtgaaaacat ggggttgcgg aggttttttg
      421 aatgagacga acatttacag cagaggaaaa agcctctgtt tttgaactat ggaagaacgg
      481 aacaggcttc agtgaaatag cgaatatcct gggttcaaaa cccggaacga tcttcactat
      541 gttaagggat actggcggca taaaacccca tgagcgtaag cgggctgtag ctcacctgac
      601 actgtctgag cgcgaggaga tacgagctgg tttgtcagcc aaaatgagca ttcgtgcgat
      661 agctactgcg ctgaatcgca gtccttcgac gatctcacgt gaagttcagc gtaatcgggg
      721 cagacgctat tacaaagctg ttgatgctaa taaccgagcc aacagaatgg cgaaaaggcc
      781 aaaaccgtgc ttactggat
//
LOCUS       DAFNBJ010000390          792 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.410, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000390 DAFNBJ010000000
VERSION     DAFNBJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.410"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>792
                     /gene="mph_3"
                     /locus_tag="L2X49_005071"
     CDS             <1..>792
                     /gene="mph_3"
                     /locus_tag="L2X49_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mph(E)/Mph(G) family macrolide
                     2'-phosphotransferase"
                     /protein_id="HBN4799991.1"
                     /translation="VVFALDTKGQQWLLRIPRRDGMREQIKKEKRILELVKKHLSVEV
                     PDWRISSTELVAYPILKDNPVLNLDAETYEIIWNMDKDSPKYITSLAKTLFEIHSIPE
                     KEVRENDLKIMKPSDLRPEIANNLQLVKSEIGISEQLETRYRKWLDNDVLWADFTQFI
                     HGDLYAGHVLASKDGAVSGVIDWSTAHIDDPAIDFAGHVTLFGEESLKTLIIEYEKLG
                     GKVWNKLYEQTLERAAASPLMYGLFALETQNESLIVGAKAQLGVI"
BASE COUNT          268 a          121 c          170 g          233 t
ORIGIN      
        1 aggtcgtttt tgctcttgat acaaaggggc aacaatggtt gctgcgtatt cctcgtcgtg
       61 atggcatgag ggaacaaatc aagaaagaaa aacgcatttt agaattggta aaaaaacatc
      121 tttctgtaga ggttcctgat tggagaattt catctacaga attagtggct tatcccatac
      181 ttaaagataa tcctgtttta aatttggatg ctgaaaccta tgaaataatt tggaatatgg
      241 acaaagatag cccgaaatac ataacatctt tggcaaaaac cttatttgaa atccatagta
      301 ttcctgaaaa agaagttcgg gaaaatgatt tgaaaattat gaaaccttca gatttaagac
      361 ctgaaatagc aaacaatttg cagttagtaa aatctgaaat tggtataagt gagcaattgg
      421 aaacccgcta cagaaaatgg ttggataatg atgttctatg ggcagatttc acccaattta
      481 tacatggcga tttatatgct gggcatgtac tagcttcaaa ggatggagct gtttcaggcg
      541 ttattgattg gtcaacagcc catatagatg acccagcgat tgattttgct gggcatgtaa
      601 ctttgtttgg agaagaaagc ctcaaaactc taatcatcga gtatgaaaaa ctagggggta
      661 aagtttggaa taaactatat gaacagactt tagaaagagc agcggcctct cctttgatgt
      721 atggtttatt tgccttagaa actcaaaatg aaagccttat cgttggagca aaagctcagt
      781 tgggagttat at
//
LOCUS       DAFNBJ010000391          777 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.411, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000391 DAFNBJ010000000
VERSION     DAFNBJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.411"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..299)
                     /locus_tag="L2X49_005072"
     CDS             complement(<1..299)
                     /locus_tag="L2X49_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799992.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYYLSGGYIQWFV"
     gene            complement(299..718)
                     /locus_tag="L2X49_005073"
     CDS             complement(299..718)
                     /locus_tag="L2X49_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799993.1"
                     /translation="MGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKG
                     DGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLG
                     LFKNCQDWSNDSLETCKMKCSGNNIGRLIRFVFTGVM"
BASE COUNT          250 a          176 c          115 g          236 t
ORIGIN      
        1 acaaaccatt gaatataccc tcctgacaga taataaagcc atattattat cgccataata
       61 tacttactat caatccttac gccaataatg tctgatacag tccctataaa tattagagca
      121 ataaagaata ctatgcctgt tggaaaacat attaccatca ttccataaga catgagacta
      181 attaactcgc tcacatcacg agtggagtct aatagtgaaa gtctatgcat ggaaaagaat
      241 agtattaaaa gcgataaagc cagccatata gctctgaata aaaaaataat atatttcatt
      301 acatcactcc ggtgaataca aatctaataa gacgtccaat gttatttccg gagcacttca
      361 tcttacatgt ttctaaagaa tcattagacc agtcttggca gtttttaaat agccctaaat
      421 ttataggtgt gaatatatca ggcaatacag aataacgcgg ccttacctct ttaaacttcc
      481 cttttaaaca gttctccaca caactattat cggtttcaac gaggtcacaa cggccagcag
      541 cttcttttgt atcattactt gctttacctg gcccccatag accatctcct tttgattctc
      601 cccgttggtc ttggccacca caagtatagt cccctttacc atctgggaca caaagatatt
      661 gatgatagaa cggattcccc catatatctg gaccgcttct ttcaccagtt ccgcccattg
      721 aatgcagtgg cttaatgcat tttagtgttt ctaatcctaa tggatctata tttgaga
//
LOCUS       DAFNBJ010000392          775 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.412, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000392 DAFNBJ010000000
VERSION     DAFNBJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.412"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            156..>775
                     /locus_tag="L2X49_005074"
     CDS             156..>775
                     /locus_tag="L2X49_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBN4799994.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDDNVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT          213 a          176 c          182 g          204 t
ORIGIN      
        1 tgcattgttt ttcaatgaaa aaatattatt cgcgtaatat ctcacgataa ataacattag
       61 gattttgtta tttaaacacg agtcctttgc acttgcttac tttatcgata aatcctactt
      121 ttttaatgcg atccaatcat tttaaggagt ttaaaatgga taagaagcaa gtaacggatt
      181 taaggtcgga actactcgat tcacgttttg gtgcgaagtc tatttccact atcgcagaat
      241 caaaacgttt tccgctgcac gaaatgcgcg acgatgtcgc attccagatt atcaatgacg
      301 aattatatct tgatggcaat gctcgtcaga acctggccac tttctgccag acctgggacg
      361 acgacaatgt ccacaagttg atggatttat ccattaacaa aaactggatc gacaaagaag
      421 aatatccgca atccgcagcc atcgacctgc gttgcgtaaa tatggttgcc gatctgtggc
      481 atgcgcctgc gccgaaaaat ggtcaggccg ttggcaccaa caccattggt tcttccgagg
      541 cctgtatgct cggcgggatg gcgatgaaat ggcgttggcg caagcgtatg gaagctgcag
      601 gcaaaccaac ggataaacca aacctggtgt gcggtccggt acaaatctgc tggcataaat
      661 tcgcccgcta ctgggatgtg gagctgcgtg agatccctat gcgccccggt cagttgttta
      721 tggacccgaa acgcatgatt gaagcctgtg acgaaaacac catcggcgtg gtgcc
//
LOCUS       DAFNBJ010000393          772 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.413, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000393 DAFNBJ010000000
VERSION     DAFNBJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.413"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..363
                     /locus_tag="L2X49_005075"
     CDS             <1..363
                     /locus_tag="L2X49_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799995.1"
                     /translation="YTYTLKENGMPYAGVIAQEVMEAIPEAVGSFTHYGEELQGPTVD
                     GNELREETRYLNVDYSAVTGLLVQVARETDDRVTALEEENTTLRQNLATAGTRISTLE
                     NQVSELVALVRQLTGSEH"
     gene            400..543
                     /locus_tag="L2X49_005076"
                     /pseudo
     CDS             400..543
                     /locus_tag="L2X49_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          238 a          145 c          196 g          193 t
ORIGIN      
        1 tacacctaca cgctcaagga aaacgggatg ccttatgctg gcgttattgc acaggaagta
       61 atggaggcga taccagaagc tgtgggatcg tttactcatt atggtgaaga gttgcaaggt
      121 ccgaccgttg acggcaacga gctacgcgaa gaaactcgct atcttaatgt tgactactcc
      181 gccgtgacgg gtttacttgt tcaggtcgcc cgtgaaacag atgatcgcgt taccgcgctg
      241 gaagaggaaa acacaacgct acgtcaaaat ctggcaacag caggcacccg gatcagcact
      301 ctggaaaatc aggtaagcga actggttgca cttgtccggc agttaacagg aagcgaacat
      361 tgatatcctt caagccctga aggaggctgt tcctggtacg ttcagactgt tgttgagctg
      421 gaaatcgcaa cggaggaaga aacttcgttg ctggaagtct ggaagaagta tcgggtgttg
      481 ctgaaccgtg ttaatacaac aactgcaccg gatattgaat ggccagtagc acctataggg
      541 taaattcgta atgattacct aaatacgtta ttcttttgtt caaaaagtga ttttgctata
      601 ggtagggagt atgttcttgg attaagaata aaaatcgcca tgaaagagct tcatggcgag
      661 tacataaacg tacaaaatat gatgaaagta gagtggcgta agccactata ttaagatatg
      721 acaatagcga agaaaagtaa ataacatttt atgggcgttt aattgttaat ta
//
LOCUS       DAFNBJ010000394          772 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.414, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000394 DAFNBJ010000000
VERSION     DAFNBJ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.414"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>772
                     /locus_tag="L2X49_005077"
     CDS             <1..>772
                     /locus_tag="L2X49_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HBN4799996.1"
                     /translation="LVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIY
                     DRRQFSNCVLSLAFLSCLIYFVKTVIIIQQIIEERLTSSVVQNDIAIYYLFRQMSLCI
                     LIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFGGPIVAHILSSHYESYNLHIAELT
                     NENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLLFM
                     SRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKN"
BASE COUNT          207 a          113 c          158 g          294 t
ORIGIN      
        1 tttggtgaat agttggttta atcagccagg cgttgaggaa gttgttccac gatcaaccta
       61 tctgatggta atgattgctt tattttttat cgatactgtt gcatttattt ttatgcagtt
      121 gtatttcatt tatgaccgta ggcaattttc aaactgtgta cttagtttgg cttttctgag
      181 ttgtttgatt tactttgtta aaaccgtcat tatcattcag caaattattg aggagcgttt
      241 gacaagcagt gttgtccaaa atgacatagc aatctattat ttgtttcgtc agatgagttt
      301 gtgcatatta atatttctgg cattggtgaa taaagttagt gaaaacacaa aacagcgcaa
      361 tttattttcc aaaaaaatga ctttgtgcat aagtttgttt tttgtttttg gggggccgat
      421 agttgctcac atactttcta gtcattatga gagctacaat ttacacattg ccgagttaac
      481 caatgaaaat ggtcaggtgg tttggaaagc ctcatatgtt accataatga ttttcatgtg
      541 gttaaccttg ctttcggtta atctatactt taatggttta cgctatgata tctggaatgg
      601 agtgacggtg attgcttttt gtgccgtact gtataatatc tctctgttat ttatgagcag
      661 gtatagcgtg tccacttggt atattagtcg cactattgaa gtagtcagta agttaaccgt
      721 tatggtaata tttatgtgcc atatattcag tgcgctacga gtaacaaaga ac
//
LOCUS       DAFNBJ010000395          770 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.415, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000395 DAFNBJ010000000
VERSION     DAFNBJ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.415"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            24..>770
                     /locus_tag="L2X49_005078"
     CDS             24..>770
                     /locus_tag="L2X49_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799997.1"
                     /translation="MDINMCNSKGQTALFTCNVPEAIQAMIDADIDIHHLDNDGNNAL
                     FYAQNVETVELLVSNGINVNHRNKSGTLAIQHIDVSPGLVKYLIKAGLDIHTKDSYGN
                     SFLFIPFEGYVYDALIEAGCDINHKNISGQTAFDHWQSENNSIVGKYTHWESKNDNYI
                     RFLIRNNHLKDSSKIVFTNITFKSIELLSLLIKQKNEFEISDKCIITPTNADAKKLIL
                     QLKKLTDISHVWFYISSTYLLTHYAGRLLIK"
BASE COUNT          270 a          135 c          135 g          230 t
ORIGIN      
        1 tccttcgttg ccttaaggct ggcatggata taaatatgtg caatagtaaa ggtcaaactg
       61 ctctttttac ctgtaatgta cctgaagcta tacaggcaat gattgacgct gacattgaca
      121 ttcaccactt ggataatgac ggtaataatg cattattcta tgctcaaaat gttgaaacag
      181 tggaacttct tgtcagcaat ggtattaatg ttaaccaccg gaataagtca ggcacccttg
      241 cgattcaaca tattgatgtt tcaccagggt tagtaaaata tcttatcaaa gcaggactag
      301 atatccatac caaagatagc tatggcaatt cctttctttt cattccattt gaaggatatg
      361 tgtacgatgc actaattgag gctggttgcg acattaatca taaaaatata agtggacaga
      421 cagcattcga tcactggcag tcagaaaata atagcatagt tggtaaatat acgcactggg
      481 aatctaaaaa cgataactac attcgttttc ttatccgtaa taatcatctt aaagacagtt
      541 caaaaattgt attcacgaac attacattta aatcaattga gctgttgtcg ttacttataa
      601 agcaaaaaaa tgaatttgag ataagtgata agtgcattat cacaccaaca aatgccgacg
      661 ctaaaaaatt aattttacaa ctaaagaagc tgacagacat tagtcatgtt tggttttaca
      721 taagcagtac atatctactg acgcactatg caggaagact gctaattaaa
//
LOCUS       DAFNBJ010000396          767 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.416, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000396 DAFNBJ010000000
VERSION     DAFNBJ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.416"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            8..>767
                     /locus_tag="L2X49_005079"
     CDS             8..>767
                     /locus_tag="L2X49_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBN4799998.1"
                     /translation="MLPEKNEFPIIQIEIAKIKFLNPRTRNKVVHEEIKESIKKRGLS
                     KPISVRAIDEGDFKYALICGQGRIEALVALGETIIPAIIRDVSEEDAYVMSLVENIAR
                     RRPRSNELLQVIKDMKIRGLSDSEISEITGYSSNWVSSINMLLDKGEHKLLSAVERGN
                     LPLYLAVQFARCETEEAQDILTEAYDKKLIKSRDIIKIKHILNQRTVGNKGAKAAGFY
                     YHKPSKRMTAEELIELYENSIAEHKSVYNNSKFIK"
BASE COUNT          307 a          108 c          156 g          196 t
ORIGIN      
        1 gaactatatg ctgcctgaaa aaaatgaatt cccaataatt caaattgaga ttgcaaaaat
       61 aaaatttctt aacccacgaa caagaaataa ggtagtgcat gaagaaataa aggaaagcat
      121 aaaaaaaaga ggattaagca agccaataag cgtaagagct attgatgaag gcgatttcaa
      181 atatgcttta atttgtggtc aagggagaat agaggctctc gttgcattag gtgaaactat
      241 tattccagca attataagag atgtatcaga agaagatgct tacgttatga gtttagttga
      301 aaacattgca aggagaagac cacgttctaa tgagttatta caggtgatta aagacatgaa
      361 aatcagagga ctttcagact ccgaaataag tgagattact ggatattcat cgaactgggt
      421 gagcagtatt aatatgctac ttgataaggg agagcataaa cttctctcag cagtcgaacg
      481 gggtaatttg cctctgtatc tcgcagtgca atttgcaaga tgtgaaactg aggaagcaca
      541 agatattctt accgaagcat atgataaaaa attaataaaa agtcgggaca ttataaagat
      601 aaaacacatt ctaaatcaaa gaacagttgg gaataaaggt gcaaaagcag ccggatttta
      661 ttatcacaaa ccatcaaaaa ggatgactgc agaggagttg attgagcttt atgaaaatag
      721 tatcgctgaa cataaatctg tttataacaa ctcaaaattc ataaaaa
//
LOCUS       DAFNBJ010000397          761 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.417, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000397 DAFNBJ010000000
VERSION     DAFNBJ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.417"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..741)
                     /locus_tag="L2X49_005080"
     CDS             complement(<1..741)
                     /locus_tag="L2X49_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4799999.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVILYNDSSLRMDFFGYNALIIQRSD
                     NTCELTINEPLKNQEISTGNINVNFPLKDIYNEIRRLNVVFSCGTGGIVDLSSLDLRN
                     IDLELYDFTDKHMANAILNPFKLDDTDFTNANMFQVNFVSSKQNTTISWDYLLKITPV
                     LTSISDMYSEEKIKLVESCLNELGDITEE"
BASE COUNT          242 a          126 c          125 g          268 t
ORIGIN      
        1 ttcttcggta atatctccaa gctcatttaa acaactttcg acaagcttaa ttttttcttc
       61 agaatacata tcgctaatgc ttgttagaac aggcgttatt tttagtaaat aatcccagga
      121 gattgtggtg ttttgttttg aactaacgaa atttacctga aacatgttgg cattagtaaa
      181 atctgtgtca tccaatttaa aaggatttaa aattgcattg gccatatgtt tatctgtaaa
      241 atcataaagt tctaaatcga tattccgtaa gtccagagag gatagatcaa ctatacctcc
      301 agtcccacaa ctaaaaacta cgttcaacct cctgatttca ttgtaaatgt ctttcaatgg
      361 gaaattaaca ttgatattgc ctgtggatat ttcctggttt tttaacggct cattaattgt
      421 aagttcacag gtattgtctg atcgttgaat aattagggcg ttataaccaa aaaaatccat
      481 acgaagagac gaatcattat aaagaattac attttgtaag tacgctaatt ctttttgaaa
      541 atcaccagtg ataatgcatt ttccacctgg ttgtaacgcg ttaagtaaag tgaccatttg
      601 aatagaggtc tgttttagtt tttctatacc tggtgctagt gcttcgaggt tattaactat
      661 ggggctttca ggaagatcat tcatttttcg acggactaaa tcactactaa tatcattacc
      721 aattgatgaa acagggaaca ttattttatc ctttataaat a
//
LOCUS       DAFNBJ010000398          759 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.418, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000398 DAFNBJ010000000
VERSION     DAFNBJ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.418"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..202
                     /locus_tag="L2X49_005081"
     CDS             <1..202
                     /locus_tag="L2X49_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBN4800000.1"
                     /translation="INKSINEIIEKTEINQLSFSVVGKKGRMAHMLRFEFSINEKSSS
                     FSEDDMAFLEEFDKVVPPKKNK"
     gene            211..654
                     /locus_tag="L2X49_005082"
     CDS             211..654
                     /locus_tag="L2X49_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800001.1"
                     /translation="MAKIYEFPQGAERSKLKKEIIRERKKRLRETNGNPVIRHAKWFW
                     FYLRLATAGALHLVSVISLAVLGAFSKAIFWIGGMLCVVTWFHLERQFWTPQNFTIPV
                     IVTLWGLSLFATPLMELLNKKMPWYRLLVPDAKHTSTEATNDDQP"
BASE COUNT          217 a          147 c          181 g          214 t
ORIGIN      
        1 tatcaataaa agcattaatg agataattga gaaaacggaa ataaatcagt tatctttctc
       61 tgttgttggg aaaaaaggga gaatggctca catgcttaga tttgagttta gtataaatga
      121 aaaatcctca tccttttcgg aagatgatat ggcattttta gaggagtttg acaaagtcgt
      181 tccaccaaaa aagaataagt gaggcaaaac atggctaaaa tatatgagtt tccgcaggga
      241 gccgaacgaa gtaagctgaa aaaagaaatc atccgggaac gtaaaaaacg attgcgagaa
      301 acgaacggta acccggttat ccggcacgct aaatggttct ggttttatct acgtcttgct
      361 acggctggcg cactgcattt agtctctgtt atttctctgg cagtcctggg ggctttcagt
      421 aaagctattt tctggattgg tggaatgctc tgtgttgtta catggttcca ccttgagcgc
      481 cagttctgga cgccgcaaaa tttcacgatt ccagtaatcg taacgctgtg gggattgagt
      541 ttgtttgcca ctcccctgat ggagctgctt aacaaaaaaa tgccgtggta tcgtcttctg
      601 gttcctgatg caaagcacac tagcactgag gcaaccaatg atgatcagcc gtgaagcaac
      661 aatgttatcc agtcggcttg cagtgcttct ttcagccttt tatcatcggt taatcatagc
      721 gcacgaggcg agttcttttc actgcgatgc aggataggg
//
LOCUS       DAFNBJ010000399          753 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.419, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000399 DAFNBJ010000000
VERSION     DAFNBJ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.419"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..515)
                     /locus_tag="L2X49_005083"
     CDS             complement(<1..515)
                     /locus_tag="L2X49_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBN4800002.1"
                     /translation="MLPTLGYAVISKHNTNINHVHAVCRFINYLINTYVSPRYMDGTP
                     KELSRYALQLSKRLSTYRSDFRPSKQKHSHKHFNSLTADMVRRFYEIIRPESSFKPNP
                     LNPFPAGEVQFRNYLICRLLLNYGLRVSELLLLEKHSIKPNIQGGQFSIIVTSVDDDV
                     RDPRKRLPSLK"
BASE COUNT          245 a          123 c          165 g          220 t
ORIGIN      
        1 ttttttaatg atggcaatcg ttttcttgga tctctgacat catcatctac agacgtcact
       61 atgatactga attgtccgcc ttggatgtta ggtttaattg agtgtttttc taacaggagc
      121 aattcactta ctcgcaaacc ataattcaaa agaagacgac agattaaata atttctgaat
      181 tgaacttctc cagcagggaa gggattaaga ggatttggct tgaaagacga ttctggtcta
      241 ataatctcat aaaatctcct gaccatgtct gctgtaaggc tattaaaatg tttgtgtgaa
      301 tgcttttgct tgctgggtcg aaaatcagaa cgataggtcg acaaacgctt ggataattgt
      361 aaagcataac gagaaagctc ttttggggtg ccatccatat aacgaggaga aacgtaagta
      421 ttgatcagat agtttataaa acggcagact gcatgtacat gatttatatt agtattgtgc
      481 ttgctaatta ctgcatatcc gagtgtaggc aacataagct ttcctgattc aagataatga
      541 agaaatgctt caagctcact aaccgcaatc gaaggattgt atccagacaa ttggaagctc
      601 aaacagaaag tgacatcatg tttttgatac cagtactcat aaaaaaactt tagagcttgt
      661 agcgtagatg actgcgtgga ttcggaacgt gaagctaagc gatcaatgag aaagagaagt
      721 ggaaacagaa gcggtaagtt agtagtggtg tca
//
LOCUS       DAFNBJ010000400          746 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.420, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000400 DAFNBJ010000000
VERSION     DAFNBJ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.420"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            131..727
                     /gene="fimE"
                     /locus_tag="L2X49_005084"
     CDS             131..727
                     /gene="fimE"
                     /locus_tag="L2X49_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBN4800003.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          195 a          145 c          182 g          224 t
ORIGIN      
        1 ttaatgtaat tgactgatag ccacatcact tcgtgtgtgg ttatcttttt atctattggg
       61 ctaattttga ccgattgagg tttcctatag gtattcattc aaatatatct cagtcaggag
      121 tactactatt gtgagtaaac gtcgttatct taccggtaaa gaagttcagg ccatgatgca
      181 ggcggtttgt tacggggcaa cgggagccag agattattgt cttattctgt tggcatatcg
      241 gcatgggatg cgtattagtg aactgcttga tctgcattat caggatcttg accttaatga
      301 aggtcgaata aatattcgcc gactgaagaa cggattttct accgttcacc cgttacgttt
      361 tgatgagcgt gaagccgtgg aacgctggac ccaggaacgt gctaactgga aaggcgctga
      421 ccggaccgac gctatattta tttctcgccg tgggagtcgg ctttctcgcc agcaggccta
      481 tcgcattatt cgcgatgccg gtattgaagc tggaaccgta acgcagactc atcctcatat
      541 gttaaggcat gcttgcggtt atgaactggc ggagcgtggt gcagatactc gtttaattca
      601 ggattatctc gggcatcgaa atattcgcca tactgtacgt tataccgcca gtaatgctgc
      661 tcgttttgcc ggattatggg aaagaaataa tctcataaac gaaaaattaa aaagagaaga
      721 ggtttgattt aactcattga taataa
//
LOCUS       DAFNBJ010000401          735 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.421, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000401 DAFNBJ010000000
VERSION     DAFNBJ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.421"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..267
                     /locus_tag="L2X49_005085"
     CDS             <1..267
                     /locus_tag="L2X49_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBN4800004.1"
                     /translation="VVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDG
                     DKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANCSRGKNL"
     gene            291..>735
                     /locus_tag="L2X49_005086"
     CDS             291..>735
                     /locus_tag="L2X49_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBN4800005.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKYLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQ"
BASE COUNT          258 a          153 c          123 g          201 t
ORIGIN      
        1 gttgtaactg gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta taacaaggat
       61 ggtaataaac tcgaccttta tggcaaggtt accgctctac gttattttac tgatgataag
      121 cgtgacgatg gtgataaaac ttatgcccgt ctcggcttta aaggagaaac gcaaatcaat
      181 gatcaaatga ttggttttgg tcactgggaa tatgatttta aaggctataa cgatgaagcc
      241 aactgctcgc gcggcaagaa tctctgattg agttaagtgc ccatcacaat atgatcactg
      301 accttatatt acacaatcat cccaggatga aaacaatcac tttaaacgac aaccatattg
      361 cacatttaaa cgccaaaaac actacaaaac tggaatattt aaacttaagc aataacaatt
      421 tactgccaac caatgacatt gatcaactaa tatcatcaaa atatctttgg catgtattag
      481 ttaacggcat caacaatgat ccacttgcac aaatgcagta ctggactgca gtaagaaata
      541 taattgatga cactaatgaa gtgaccattg atttatcagg acttaattta accactcaac
      601 caccagggct gcaaaacttc acctctatca atcttgataa taaccaactc acacattttg
      661 atgcaaccaa ctacgataga ctcgtaaagc taagtctgaa tagtaatgct cttgagtcaa
      721 taaattttcc tcaag
//
LOCUS       DAFNBJ010000402          717 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.422, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000402 DAFNBJ010000000
VERSION     DAFNBJ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.422"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>717
                     /locus_tag="L2X49_005087"
     CDS             <1..>717
                     /locus_tag="L2X49_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015741777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBN4800006.1"
                     /translation="AIEHFEHDVGNLVLRKIRSALYPKLSAVVSQTGEDQIKYLQWLP
                     RKKHKIIPNIISFEATDIPQNKIEQKNVLAVGRLTHQKGFDLLLQAWADANTHDWRLK
                     IIGDGEELNHLNSLITELNISNAEIIPFQKDIQRHYSSAGIFVLSSRFEGLGMVLLEA
                     LSSGLACISFDCPAGPKSIISSDNGVLVPTGDTIKLSQAISFLINNEEERKRLQNKAA
                     ASVEKFKESNVIAKWRELLN"
BASE COUNT          245 a          113 c          141 g          218 t
ORIGIN      
        1 tagctattga gcattttgaa catgatgttg gtaatttagt gttgagaaaa attagaagcg
       61 cactatatcc taaattatca gcagttgtat cgcaaactgg cgaagatcaa attaagtatt
      121 tgcaatggtt acctagaaag aaacataaaa tcattccaaa tattattagc ttcgaagcga
      181 ccgatatacc gcaaaataaa atagaacaaa aaaatgtact tgctgtggga cgattaactc
      241 atcaaaaggg ttttgattta cttctacaag cttgggcaga cgcaaatact catgattggc
      301 gcttaaagat cattggagac ggagaagagc tgaaccattt aaattctcta attaccgagt
      361 taaatatctc taacgctgaa attatccctt ttcaaaagga tattcaaagg cattattctt
      421 ctgcaggaat attcgtactt tcttctcgct ttgagggttt gggtatggtg cttttagaag
      481 ctcttagcag cggcttggcg tgtattagtt ttgattgtcc agctggtcct aaaagtataa
      541 tctcaagtga taatggggtg ttagttccaa ctggtgacac tataaaatta tcacaagcta
      601 tttctttctt gataaataac gaagaagaaa gaaaacggtt acaaaacaaa gctgctgctt
      661 ctgttgaaaa attcaaagaa tcaaacgtaa ttgcaaaatg gcgagagtta ttgaatg
//
LOCUS       DAFNBJ010000403          680 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.423, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000403 DAFNBJ010000000
VERSION     DAFNBJ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.423"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>680
                     /locus_tag="L2X49_005088"
     CDS             <1..>680
                     /locus_tag="L2X49_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HBN4800007.1"
                     /translation="ALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKRAH
                     TGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQD
                     VMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRAC
                     NGLSDNRCL"
BASE COUNT          169 a          162 c          211 g          138 t
ORIGIN      
        1 gcgctgcttc gcagacaggc agaacttgat ggtatgcctg cgatcaatgc caaacgtgtt
       61 taccggatca tgcgccagaa tgcgctgttg cttgagcgaa aacctgctgt accgccatcg
      121 aaacgggcac atacaggcag agtggccgtg aaagaaagca atcagcgatg gtgctctgac
      181 gggttcgagt tctgctgtga taacggagag agactgcgtg tcacgttcgc gctggactgc
      241 tgtgatcgtg aggcactgca ctgggcggtc actaccggcg gcttcaacag tgaaacagta
      301 caggacgtca tgctgggagc ggtggaacgc cgcttcggca acgatcttcc gtcgtctcca
      361 gtggagtggc tgacggataa tggttcatgc taccgggcta atgaaacacg ccagttcgcc
      421 cggatgttgg gacttgaacc gaagaacacg gcggtgcgga gtccggagag taacggaata
      481 gcagagagct tcgtgaaaac gataaagcgt gactacatca gtatcatgcc caaaccagac
      541 gggttaacgg cagcaaagaa ccttgcagag gcgttcgagc attataacga atggcatccg
      601 catagtgcgc tgggttatcg ctcgccacgg gaatatctgc ggcagcgggc ttgtaatggg
      661 ttaagtgata acagatgtct
//
LOCUS       DAFNBJ010000404          664 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.424, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000404 DAFNBJ010000000
VERSION     DAFNBJ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.424"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>664
                     /locus_tag="L2X49_005089"
     CDS             <1..>664
                     /locus_tag="L2X49_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBN4800008.1"
                     /translation="HRNTAYQALKDACDDLFARQFSYQSLSEKGNTINHKSRWVSEVA
                     YIDNEAVVRLIFAPAIVPLITRLEEQFTKYEIQQISNLTSAYAVRLYEILIAWRSTGK
                     TPLITMYDFRQKIGVLETEYKRMYDFKKYVLDIALKQVNEHTDIIVKVEQHKTGRSIT
                     GFSFSFKQKKSATHSVESKRDPNTLDLFSKITDKQRHLFANKLSELPEMSKYSQGTES
                     YQ"
BASE COUNT          246 a          108 c          116 g          194 t
ORIGIN      
        1 tacatcgaaa tacggcttat caggcgttaa aagatgcttg cgatgacttg tttgcaagac
       61 aattcagtta tcagagtctt agtgaaaaag gtaacactat taatcacaaa tcaagatggg
      121 tgagcgaggt ggcttatatt gataatgaag ctgtcgttag acttattttt gcccctgcta
      181 ttgtgccttt aattactagg ttagaagaac aatttacaaa gtatgaaata caacaaataa
      241 gtaatttaac aagtgcttat gctgttcgtt tatatgaaat attgattgca tggcgtagta
      301 ctggaaaaac gcctctcata actatgtatg attttagaca aaaaataggt gtactcgaga
      361 ctgaatacaa acgaatgtat gattttaaaa aatatgtttt agacattgca ttaaaacaag
      421 taaatgaaca taccgatatt attgtcaaag ttgaacagca taaaacaggt agatctatta
      481 ctggtttttc atttagcttt aaacagaaaa aatcagccac gcattcagtc gaatctaaaa
      541 gagatccgaa tacattagac ctcttttcaa aaataacaga taaacaacgc catctattcg
      601 ccaacaagct ctcagagctt cctgagatga gtaaatattc acaaggcaca gaaagctatc
      661 agca
//
LOCUS       DAFNBJ010000405          662 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.425, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000405 DAFNBJ010000000
VERSION     DAFNBJ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.425"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>662
                     /locus_tag="L2X49_005090"
     CDS             <1..>662
                     /locus_tag="L2X49_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HBN4800009.1"
                     /translation="TISNEVAHSLTLYCWSHYGFSPEGDVPSLEYIRGVRNIYTYNDK
                     EEGHEKVWLNTLLKYGYRKTNDLDEVLIDMVRYGYLDKSSFQRQISLRNEEITRDNKR
                     GSLSEAWQFFHNSFDDNQNQVVDKLYQAVVDGMKYVTPSDLDNVVGLYRDFGENAKAS
                     ELIEQFINYGNDALKEYVQSIYVNVHPVRDTELLSKIQAYSKIINIDGSIYDVLKKLS
                     GQ"
BASE COUNT          222 a           83 c          149 g          208 t
ORIGIN      
        1 gacaatttca aatgaagttg cgcattcatt aactctgtat tgttggagcc attacggatt
       61 ttctccagag ggagatgtgc catcactaga atatattagg ggagtgagga atatatatac
      121 ttataatgac aaggaagaag gccatgagaa agtctggctt aataccttgc ttaaatatgg
      181 atatagaaaa acgaatgatc ttgatgaggt tcttattgat atggttagat atggctatct
      241 tgataagagc agtttccaaa gacaaataag cctaaggaat gaggagatta cgcgtgataa
      301 taaacgagga tccttatctg aggcatggca gttctttcat aattcatttg atgacaatca
      361 aaatcaggtt gttgacaagc tatatcaagc ggtggttgat gggatgaaat atgtgactcc
      421 aagtgattta gataatgttg tcggactata tagggatttc ggagagaatg ctaaggcgag
      481 tgaattgatt gaacagttta ttaattatgg taacgatgct ctaaaggaat atgttcaatc
      541 tatttatgtg aatgttcatc ccgtaagaga tactgagttg ttgtcaaaaa ttcaagccta
      601 ttctaagatt ataaatattg atggttcaat atatgatgtt cttaagaaat tatcaggtca
      661 ga
//
LOCUS       DAFNBJ010000406          651 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.426, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000406 DAFNBJ010000000
VERSION     DAFNBJ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.426"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..648
                     /locus_tag="L2X49_005091"
     CDS             <1..648
                     /locus_tag="L2X49_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HBN4800010.1"
                     /translation="SISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQEAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLGKDFDTIYTLKNELHDRSHRKFQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          219 a          119 c          125 g          188 t
ORIGIN      
        1 agtatttcca cactgctgta tctggatgcc gatgttgttt gtaaaggaag cctgaaacct
       61 ctcacagaaa ttatatttaa agatgagttt gctgcggtca ttcctgacaa tgatagtacg
      121 caggaggcat gtgcaaaacg cctcaacatt cccgaaatga atggacgtta tttcaatgca
      181 ggcgttatct atgtcaatct taaaaaatgg catgaagcaa atttgacacc gtatttactc
      241 aagcttttac gaggggaaac taaatatggc tctcttaaat atttagatca ggatgcgttg
      301 aatatcgcat ttaatatgaa taatatctac ctcgggaagg attttgatac tatttatacc
      361 ctgaaaaatg aacttcatga tcgtagtcat cgaaagtttc agcaaaccat taccgataaa
      421 acagtattga ttcactatac agggataact aaaccatggc atagctgggc tggatatccg
      481 tctgcatcat actttaatat cgcgcgtgaa caatctccct ggaagaaata tcctcttaaa
      541 gaggcgcgga ctgttgcaga aatgcagaaa caatataagc atctgtttgc ccatggtgag
      601 tatataaaag gcataacttc attaattaag tacaagctta agaaataaat t
//
LOCUS       DAFNBJ010000407          647 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.427, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000407 DAFNBJ010000000
VERSION     DAFNBJ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.427"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            75..557
                     /gene="nanC"
                     /locus_tag="L2X49_005092"
                     /pseudo
     CDS             75..557
                     /gene="nanC"
                     /locus_tag="L2X49_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
     gene            complement(575..>647)
                     /locus_tag="L2X49_005093"
     CDS             complement(575..>647)
                     /locus_tag="L2X49_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBN4800011.1"
                     /translation="SFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          191 a          124 c          144 g          188 t
ORIGIN      
        1 attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct gtttcctaca
       61 aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt
      121 cgtcaccatt aatttctcaa gctgcgacac tggacgtacg tggtggatat cgtagtggaa
      181 gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa
      241 gtatggaaag taatacctag aataccattc atgataataa aaaggaaaat gccgcactca
      301 atgatgttca ggttgatgtt aattacgcga ttaaacttga tgatcaatgg acggtgcgcc
      361 cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt
      421 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
      481 accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat
      541 atgtcactta ccatattaag gtaatgactc caacttattg atagtgtttt atgttcagat
      601 aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgac
//
LOCUS       DAFNBJ010000408          613 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.428, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000408 DAFNBJ010000000
VERSION     DAFNBJ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.428"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>613
                     /gene="ygeH"
                     /locus_tag="L2X49_005094"
     CDS             <1..>613
                     /gene="ygeH"
                     /locus_tag="L2X49_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HBN4800012.1"
                     /translation="GLTLTEDGILTQGNEQVYIPQKELGVLIVLLESAGHVVLKDMII
                     ESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFSGQVFKTKINEDNT
                     SDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAATNFCNDHISQNSF
                     LSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVD"
BASE COUNT          205 a          109 c          106 g          193 t
ORIGIN      
        1 ggattaacgc tcacggaaga tggaattctt actcaaggaa atgagcaagt ttatattcca
       61 cagaaagagt taggtgtatt aatagtatta cttgaatccg ctggtcatgt tgtactgaaa
      121 gatatgatca tcgaatcagt atggaaaaat attattgtta gtgacgagtc cctgacaaga
      181 tgtatctatt ctttgcgctg catttttgaa aaaattggct atgatcgttg catagaaaca
      241 atctaccgga aaggttatcg tttcagcggg caggttttca aaactaaaat taatgaagat
      301 aatacttcag actattccat agctatattc cctttcacta cttcattgaa tacactggat
      361 ccattaatac ttaatcagga attagtgcaa atcatttcaa ataaaaaaat cgatggtctc
      421 tatacctatc cgatggctgc gacaaatttt tgtaatgatc acatatctca aaattcattc
      481 ttgagcagat tcaagccaga ttatttcgtt acaggaagaa taaaccagaa taatgcagtg
      541 aacactttat acattgagtt gatcgacgct aaaaaccttt tcctcatcgc cagtaatcat
      601 ctccctgttg atg
//
LOCUS       DAFNBJ010000409          612 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.429, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000409 DAFNBJ010000000
VERSION     DAFNBJ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.429"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            25..>612
                     /locus_tag="L2X49_005095"
     CDS             25..>612
                     /locus_tag="L2X49_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBN4800013.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTE"
BASE COUNT          202 a          109 c          137 g          164 t
ORIGIN      
        1 taaattttcg aggagatcat tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa
       61 acggctaaag acggtgcaac gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct
      121 gctgctaaac aaggtgaacc cgatccagaa ttaaacacat ctttaaaatt cgttattgaa
      181 cgtgcaaagc aggcacaagt tccaaagcac gttattgata aagcaattga taaagccaaa
      241 ggcggcggag atgaaacgtt cgtgcaggga cgttatgaag gctttggtcc taatggctca
      301 atgattatcg ccgagacatt gacttcaaat gttaaccgta cgattgctaa cgttcgcaca
      361 attttcaata aaaaaggcgg caatatcgga gcggcaggtt ctgtcagcta tatgtttgac
      421 aatacgggtg tgattgtatt taaaggatca gaccctgacc atatttttga aattttactt
      481 gaagctgaag ttgatgttcg tgatgtgact gaagaagaag gtaacattgt tatttatacc
      541 gaacctactg accttcataa aggaatcgcg gctctaaaag cagctggaat cactgagttc
      601 tcaacaacag aa
//
LOCUS       DAFNBJ010000410          611 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.430, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000410 DAFNBJ010000000
VERSION     DAFNBJ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.430"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(32..>611)
                     /locus_tag="L2X49_005096"
     CDS             complement(32..>611)
                     /locus_tag="L2X49_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBN4800014.1"
                     /translation="DHSTIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNG
                     RWAYLYRAVDSRGRTVDFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYG
                     RALALLKREGRCPSDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGI
                     EVMRALRKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT          135 a          162 c          161 g          153 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
      481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
      541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
      601 tggagtgatc g
//
LOCUS       DAFNBJ010000411          610 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.431, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000411 DAFNBJ010000000
VERSION     DAFNBJ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.431"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(340..579)
                     /locus_tag="L2X49_005097"
     CDS             complement(340..579)
                     /locus_tag="L2X49_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V"
                     /protein_id="HBN4800015.1"
                     /translation="MPRHYEIDSAWRASIKREPNGRQTVTTEAFVSQLALINFHWSCR
                     QANQWIETYVTVFKDISTQEGENRTFMLFNPNGGR"
BASE COUNT          152 a          163 c          140 g          155 t
ORIGIN      
        1 caatgtgcgt aatgaccccc ctgacctcca acccgtcctc atcgtcatca ggtatagccc
       61 tgaagttatc cagcttatcg agttcctgga gacaccgctt ggggtgtagt cgcaatcgca
      121 tgatcctgaa ttcttgttcc aggacacaga gcaccaaaga cccgtcaacg ggcgtgcccc
      181 caaaatctac tatgagcagc gcaccaggct ttatcccttc ccggaaagag taggtatcac
      241 ttcgaaaaaa gaatactccc ggcgctcctg tattacagtg ctgatcgagt gacagcctgt
      301 tttcctggta gtcggttgct ggcgatggaa aacccatttt taacggcctc cgttggggtt
      361 aaaaagcatg aatgtccggt tctctccttc ctgagtggaa atgtctttaa aaacggtcac
      421 gtaggtttca atccactgat ttgcttggcg acaactccag tgaaaattaa tcagcgcaag
      481 ctggctgaca aaggcttctg tcgtaactgt ctgacgaccg ttcggctcac gcttaatgct
      541 cgcacgccag gctgagtcaa tttcatagtg tctaggcatg aaaaacctcc gatcaatata
      601 gctgtatgca
//
LOCUS       DAFNBJ010000412          598 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.432, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000412 DAFNBJ010000000
VERSION     DAFNBJ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.432"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..482
                     /locus_tag="L2X49_005098"
     CDS             <1..482
                     /locus_tag="L2X49_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBN4800016.1"
                     /translation="TIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIYAYAF
                     DYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYNYSPY
                     DDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSEDNG"
BASE COUNT          222 a           95 c           98 g          183 t
ORIGIN      
        1 aaacaattga aatattcaat aatagtgatg aatgggcaaa tcaactaaaa cacgcattat
       61 cgaaaggaga aaatctggca ttactacatg gtttaactcc tgatatcctt gatagaatat
      121 atgcatatgc attcgactac catgaaaagg gtaatataac ggacgcagaa atttattata
      181 aatttttgtg catttatgcg ttcgaaaatc atgagtatct aaaagatttt gcatcagtat
      241 gtcagcccaa aaagaaatat caacaagcat atgaccttta caaactaagt tacaattact
      301 ccccgtatga tgactattca gttatttatc gtatgggtca atgtcagatt ggggctaaaa
      361 atatcgataa cgcaatgcaa tgtttctatc acattattaa caattgtgag gatgatagtg
      421 ttaagagtaa agcgcaggca tatattgaac tcttaaacga taattcagaa gataatggct
      481 aagcattaca atcatttggt taaatttgct ggttatagtg cagttaacca ctataaccag
      541 cacctcttat gttttcttaa atcagcatct taaaacagtt aagagcatac tataaatc
//
LOCUS       DAFNBJ010000413          595 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.433, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000413 DAFNBJ010000000
VERSION     DAFNBJ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.433"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            42..530
                     /gene="eco"
                     /locus_tag="L2X49_005099"
     CDS             42..530
                     /gene="eco"
                     /locus_tag="L2X49_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBN4800017.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT          173 a          136 c          163 g          123 t
ORIGIN      
        1 tcatcggtga aaacctataa atgaagaagg aaagcaaaaa aatgaagacc attctacctg
       61 cagtattgtt tgccgctttc gctaccactt ccgcctgggc ggcagaaagc gtccagccac
      121 tggaaaaaat cgcgccttat ccacaagctg aaaaagggat gaagcgtcag gtgattcagt
      181 taaccccgca agaagatgaa tctaccctga aagtagaact gttaatcggt cagacgctgg
      241 aagtcgattg caatttgcat cgtctcggcg ggaagctgga aagcaaaacg ctggaaggct
      301 ggggctatga ttattatgtc tttgataaag ttagctcgcc ggtttccacg atgatggcct
      361 gcccggatgg caagaaagag aagaaatttg tcaccgcgta tctgggcgat gctggaatgc
      421 tgcgttacaa cagcaagctg ccgatcgtgg tgtatacgcc agacaatgta gatgtgaagt
      481 accgcgtctg gaaggcggaa gagaaaattg acaacgcggt agttcgctaa actgccgtga
      541 agtgcggcac cccgtaggtc agacaaggcg gtcacgccgc atccgacatc caacg
//
LOCUS       DAFNBJ010000414          592 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.434, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000414 DAFNBJ010000000
VERSION     DAFNBJ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.434"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..386
                     /locus_tag="L2X49_005100"
     CDS             <1..386
                     /locus_tag="L2X49_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBN4800018.1"
                     /translation="NSKEITEHVFHTTKFSGDEKYIKFHCNSVEELKPSKLDVIAKPG
                     DILIARVGRNFHKKILFVESGYSYISDCIFLIRASGGDKKKLFDFLCSQDGQEELSRA
                     SSGVAAQHITMDALKKIHLVRIKHD"
     gene            379..>592
                     /locus_tag="L2X49_005101"
     CDS             379..>592
                     /locus_tag="L2X49_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBN4800019.1"
                     /translation="MTDVELDSNFLIEERADYIDEDTIKNNTIRTGEFFDIIHNALIS
                     PGIKLIVGPRGCGKTHLMRYAWVECKN"
BASE COUNT          204 a           77 c          123 g          188 t
ORIGIN      
        1 ttaattcaaa ggaaattacg gaacatgttt ttcatacgac aaaatttagt ggtgatgaaa
       61 agtacattaa atttcactgc aactctgtag aagaattgaa gccatcaaaa ttagatgtca
      121 ttgctaagcc tggtgatata ttaatagcaa gagttgggcg aaattttcat aaaaaaatat
      181 tgtttgttga gagcggctat tcttatatca gtgactgtat ttttctgata cgagcctctg
      241 gtggagataa gaaaaaacta tttgattttc tttgttctca agatgggcaa gaggaattat
      301 ctcgagcgag tagtggtgta gccgcacaac atattacaat ggatgcatta aaaaaaatac
      361 atcttgtaag gattaaacat gactgatgtg gaattagata gtaattttct tatcgaggaa
      421 cgagctgatt atattgatga ggatacgata aaaaataata ctatcagaac tggagagttt
      481 ttcgatatta tacataatgc tcttatatcc ccaggcatca aacttatagt tggtcctagg
      541 ggatgtggta aaacacattt gatgcgttat gcttgggttg aatgtaaaaa cg
//
LOCUS       DAFNBJ010000415          580 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.435, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000415 DAFNBJ010000000
VERSION     DAFNBJ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.435"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            28..>580
                     /locus_tag="L2X49_005102"
     CDS             28..>580
                     /locus_tag="L2X49_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4800020.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRI"
BASE COUNT          179 a          136 c          108 g          157 t
ORIGIN      
        1 ggaagaaaaa tataaccaga gtagtgtatg gccaatctct acgacttgaa aaagttcgac
       61 ttaaaccttc ttgtcatatt tgagtgtatt taccagcatt taagcattag caaagctgct
      121 gaatcactat atatcacccc atcagccgtg agccagtcgt tacaacgctt acgtgcgcaa
      181 tttaatgacc ctttattcat tcgctcagga aaaggtatcg cgccgacgac aacagggttg
      241 aatctgcatc accatcttga gaaaaacctc aaaggccttg aacaaacaat taatatagtc
      301 aataagtccg aactaaagaa aaactttatt atctacggcc ctcagctcat ttcttgctct
      361 aataatagta tgctgatccg ctgcctacgg caagatgcct ctgtcgagat tgagtgccat
      421 gatatcctca tgtcagcaga gaatgctgaa gaactgttgg ttcaccgcaa agccgatctg
      481 gtgatcaccc aaatgccagt gatcagccgt tccgtcattt gcatgccatt gcatacaatt
      541 cgcaatactc taatttgtag taataagcat ccgcgaatta
//
LOCUS       DAFNBJ010000416          577 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.436, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000416 DAFNBJ010000000
VERSION     DAFNBJ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.436"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(343..531)
                     /locus_tag="L2X49_005103"
     CDS             complement(343..531)
                     /locus_tag="L2X49_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800021.1"
                     /translation="MQDYLLESLKLQRIDFFIKLVAASECSDEEKRLAIQWVSELTDE
                     LMAKIRSHEYSRSTEVSS"
BASE COUNT          185 a          137 c           88 g          167 t
ORIGIN      
        1 aaagatttta caaaacaata agttacaaat caaacgcgca atcacatgtt gactgacatc
       61 acatatttaa actgaagtag taacttcatt tgccttatag tctcatgtaa caattacact
      121 gttcctttag agaaggaacc ttatctctat gagaactgtt atataaacac ttgatgtaaa
      181 aaagctcaat accatagcta ccgcccatga ggctacagat tgctgcttgg ggatatagaa
      241 agcacaaatt atagtaacga tctgattcaa tagagttcat ttccatcaag ctaatcttct
      301 agcttttatc gatcattatt tcaatgcaca tacagactcc ctttaactgg aaacttccgt
      361 tgaccggctg tattcatggc tgcgaatttt cgccatcagc tcgtctgtca gttcggacac
      421 ccactggata gccagccgct tttcttcgtc gctgcactca ctagccgcta caagcttgat
      481 aaaaaaatca atacgctgaa gcttcaatga ctccaaaaga tagtcctgca tcttccctcc
      541 tatcattaca cggatacaca aaaactgtat atacacc
//
LOCUS       DAFNBJ010000417          572 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.437, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000417 DAFNBJ010000000
VERSION     DAFNBJ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.437"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..317
                     /gene="tnpA"
                     /locus_tag="L2X49_005104"
                     /pseudo
     CDS             1..317
                     /gene="tnpA"
                     /locus_tag="L2X49_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          169 a          138 c          121 g          144 t
ORIGIN      
        1 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
       61 aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac tatcctccca
      121 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg tgactgcgac
      181 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtgctggct
      241 attttgccag tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
      301 agcaaacacc tggttaggtg gaaaaccgcg ccttatatcc ctgccctgaa ggacggggtt
      361 ttacggcgca ccggataagc ctggagatta tctacaccag catggacatc attgtactat
      421 gccccgtaca tgccccatca catcctcaac atcaaagtat tagagccttg caaaccatta
      481 tgcgtctcag tttgtctcat ctatcttacg caaaaaaaaa tagaactaat atcaagcggc
      541 gctatattgc gtattaaata aaaccacctc aa
//
LOCUS       DAFNBJ010000418          570 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.438, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000418 DAFNBJ010000000
VERSION     DAFNBJ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.438"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
BASE COUNT          197 a          107 c          117 g          149 t
ORIGIN      
        1 cttactgaaa acagcaagtc atagtcatgg ttttatatag cctaaataaa ccaagaagta
       61 tacttacaca acgaccttca cgccgaagaa gctgtcagat acgccagcaa ccaaagcatt
      121 acgtttaagt gtcagtcatt gatacgaagg gataactgcg tatcagcagc cggacattga
      181 gtcgaataaa ggctggtaga gagggacagt tctgtgtcac cgggagattc gtcagaaaca
      241 tcagcggcag aacagatgaa ccagcaattg gtagtgcaca gaaaactctc cagtatttaa
      301 ttattcaact taacactaaa aaaaatcaca tagttaatat cataattttt ctttggtcta
      361 cgtatgcgag agatattgtg gctttaaccg ggtattagga gtcgtttcag ataaaagatg
      421 ttcttcgatt cgactggact tggtaatagc aataagtaat cgttaatatt gaaggacgaa
      481 aggctacttc aaaacaaagt aacctttcca atattcccat ctatgggaca agtggtacat
      541 taactgcaca tagtgcagga tacatatgac
//
LOCUS       DAFNBJ010000419          568 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.439, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000419 DAFNBJ010000000
VERSION     DAFNBJ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.439"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>568
                     /locus_tag="L2X49_005105"
     CDS             <1..>568
                     /locus_tag="L2X49_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800022.1"
                     /translation="DNQEPIELPANYKDKELVRTIINDNIVEKTHDINNKEMIFSALK
                     EIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNG
                     NDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARNASAWFESSKAIMAESFATGSD
                     HQVINELNGERLREPNEVFKRLGRAIRY"
BASE COUNT          214 a          101 c          114 g          139 t
ORIGIN      
        1 tcgacaacca agaaccaata gaattacctg cgaactataa agacaaagaa ctcgtccgta
       61 ctatcattaa tgacaacata gttgagaaga ctcatgatat caataacaag gaaatgatct
      121 tcagcgcatt aaaagaaata tatgatggag atcctggttt tatcttcgat aagatatcac
      181 acaaactcag acatacggta acggaatttg atgagagcgg gaatagcgaa ccaacggact
      241 tatttacctg gtacggtaaa gataaaaaag gcgactctct cgctattgta attaaaaata
      301 aaaacggaaa tgattactta tctctcggtt actacgatca ggacgactac cacattcaaa
      361 gaggaattcg tattaatggt gatagtctca cccaatattg tagtgaaaaa gccaggaatg
      421 cttcagcgtg gtttgaaagc agtaaagcta tcatggcaga atcatttgca acaggttccg
      481 atcatcaggt tataaacgag ctcaacgggg aaagactgag agaacctaac gaagttttta
      541 aacgtttagg tcgagcaata agatataa
//
LOCUS       DAFNBJ010000420          568 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.440, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000420 DAFNBJ010000000
VERSION     DAFNBJ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.440"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..364)
                     /locus_tag="L2X49_005106"
     CDS             complement(<1..364)
                     /locus_tag="L2X49_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800023.1"
                     /translation="MSAIENQETVIQAVNSLHPTTLFHFTKNEEAFYSILTELYFKPF
                     LARERIVGVKGRRNFAVPMVSFCDIKLSQIKDHSGKYGEFGFGLTKEWAEKNDLHPVL
                     YMNQGSELFSKYNKRIRKL"
BASE COUNT          169 a          102 c           88 g          209 t
ORIGIN      
        1 ttagttttct aattctttta ttatactttg aaaataattc actgccttga ttcatgtata
       61 gaacgggatg gagatcattt ttctcagccc attcttttgt taacccaaat ccaaattcac
      121 catattttcc actatgatct ttaatctgag aaagcttgat gtcacagaat gaaaccattg
      181 gtactgcgaa atttcttctt ccttttactc cgactatgcg ttcacgggca agaaatggtt
      241 taaaatagag ttctgttaaa atgctataga atgcctcttc atttttagta aagtgaaaca
      301 acgttgttgg atgtaaacta ttaactgctt gtataactgt ttcttggttt tctatagcac
      361 tcatatatta actgttccat agtgtaaaac ctgacttagc aaaggttatc atactagtct
      421 ttgtactgta agcacacttg gtttaattcc acgcactttt tgagatttcc ggttttttga
      481 tctcttacca aaatctatgc cagattgaat caaaggtccg agctgggtca ccaaaaaaag
      541 taaaattatg cactttgata gattatat
//
LOCUS       DAFNBJ010000421          565 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.441, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000421 DAFNBJ010000000
VERSION     DAFNBJ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.441"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(1..69)
                     /locus_tag="L2X49_005107"
                     /pseudo
     CDS             complement(1..69)
                     /locus_tag="L2X49_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
     gene            302..406
                     /locus_tag="L2X49_005108"
                     /pseudo
     CDS             302..406
                     /locus_tag="L2X49_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          185 a          112 c          113 g          155 t
ORIGIN      
        1 cctcccgaac tcaaagctat catacagata attactggtt aaccctatat agggcatttt
       61 aaagtgttgt gatcttaccc agcaatagtg gacacgcggc taagtgagta aactctcagt
      121 cagaggtgat tcacaagaca aaacagtttc aaccagtaaa aaaacgtaaa caccattcgc
      181 atgaatttcg tagttaagat ctgaagcttg cagaatgcat cggtgttact gccggagttc
      241 gtgaacttag tctgcatgaa tcaccacagc tttacatctg tcgcagtaaa cagcaaaatc
      301 agcaaactta cttaattata tcgagtgcga ttacaatcgc tggcgatggt acagttggtg
      361 tggcggtctc agtccggaac aatttgaaaa tcagaatctc acttaaaacg gcatccattt
      421 tacaaaagat ggccgtctgt gtatttgtaa tgaatacctt ctacatgttt agagtgcata
      481 atccacgaca aggcgatatc ctaaaaatta gctagcaggc aactttgatg gcatagataa
      541 tgtttttagt aaaaaagagt tgact
//
LOCUS       DAFNBJ010000422          552 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.442, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000422 DAFNBJ010000000
VERSION     DAFNBJ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.442"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
BASE COUNT          168 a           93 c          122 g          169 t
ORIGIN      
        1 tataagtaat ttatttagag tataaacaag gaaagatttg caagataact cagtgggcgt
       61 ggtgaaaata agtgtttacc cgccatcagg ctgagcataa ttctcatcat gaaatatgtt
      121 tcctggtttg tggcttgtag ctggtcactc ctgaagtctc tctggagagg cttgttgatg
      181 ttggtgtttt caggatgatg tttcacttag cttgtttgcc gaatagcccg gcgaacagct
      241 gtgattgagg aaggtttaag tcgcagtgac caaagccata tttaccaacg gatgcagatg
      301 aaaaaatcat ctcctgcttt cccccatatc tccaggataa aaaggaatgt aacaatctca
      361 tgaactaaac tgacgaacca gccaggaata atcgctaggg acctaagaat tagcctgata
      421 atagctacta agaaattact gcgttccatg aaatagccat tttgtggcaa atggagttgt
      481 ctaataatgt catatgtgag acggatagtt gaacgaatat taaattttgc tgaatttttt
      541 atgttgattt ta
//
LOCUS       DAFNBJ010000423          542 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.443, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000423 DAFNBJ010000000
VERSION     DAFNBJ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.443"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            388..495
                     /locus_tag="L2X49_005109"
     CDS             388..495
                     /locus_tag="L2X49_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBN4800024.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          139 a          127 c          165 g          111 t
ORIGIN      
        1 aagcggaagt agcgtgtcat gtgggcgtca ggctgccgta atggcaattt gcgcccggac
       61 caggccgcag gagtgaaact ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc
      121 ggataatatt ctgcggaatg cgcagcacag agtgaagatg agacggattc cggaacagaa
      181 aaacagtcgt agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg
      241 gcttaatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
      301 cacctgacaa caccacgggg gccattcttg catccaaaca atacaagaat agccgcgaaa
      361 cgttgtcatt acaatacagg cggctatatg acgctcgcag aactgggcat ggccttctgg
      421 catgatttag cggctccggt cattgctggt attcttgcca gtatgatcgt gaactggctg
      481 aacaagcgga agtaacgtgc catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg
      541 ga
//
LOCUS       DAFNBJ010000424          535 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.444, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000424 DAFNBJ010000000
VERSION     DAFNBJ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.444"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..>535)
                     /locus_tag="L2X49_005110"
     CDS             complement(<1..>535)
                     /locus_tag="L2X49_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HBN4800025.1"
                     /translation="AALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAE
                     EARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYG
                     WTPRGELAAVYDRSNTQV"
BASE COUNT           87 a          188 c          168 g           92 t
ORIGIN      
        1 cacctgtgta ttgctgcggt catacactgc cgccagttcg ccgcgcggcg tccagccata
       61 gcgcaccagc ggcgcggcag gtaaattctc cgggtattcc gggtcgtgcg tcagccacac
      121 ggcagacaga cggatgccgt tgtcccggcc atattcggtg taacccggca gggtatcagg
      181 aaaagcggac ggttccgtcc cgccggaaat ggcctgctgc cgggcttctt ctgcccgctg
      241 cgcctgcgtg gtcagtacca gccggaagtg acgcccggca ccatccgtca cgccggtgat
      301 ttcgccgctg aattcaccgg cggcttcgcg gtggaacgtc tgtgtgcgcc cgaagcggtc
      361 caccagcccg gtcagtaccc ggtacggcgg cagcggcgca ggcagcacct catccgcttc
      421 cggcacccgc tcacaccagc cgagcagcca ccacggcccc tgcggactgt ttgtcgccag
      481 ataacgatgc ggacttaagc ggagttcttc cggcagcgcc tgccagagtg cggcc
//
LOCUS       DAFNBJ010000425          534 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.445, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000425 DAFNBJ010000000
VERSION     DAFNBJ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.445"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(209..>534)
                     /locus_tag="L2X49_005111"
     CDS             complement(209..>534)
                     /locus_tag="L2X49_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBN4800026.1"
                     /translation="VSITPHVTRHTWAYLILKRIYAAKYQSVMRNCKLAGVDFAIAGL
                     MSEAKDELRLLGGWSHNSRMPEFYARRFLSERANFSNVTRIAADSSEDEFFSLIHQAF
                     GEVIK"
BASE COUNT          165 a          108 c           97 g          164 t
ORIGIN      
        1 taatcatccg ttttgaaatt aatgtgtgac tggctacctg agtaatggaa cacccaatca
       61 ttagtctctg caaatatatt cttgtcatca aaaacatcag ctgaccttaa gtggattttt
      121 gaagggatgt agagactttt tatttgtatc atttgttcac gtgaaaaaaa gacatgctta
      181 tcttcgcttt ctattgctgg gattttattc atttgataac ctccccaaac gcttgatgta
      241 tcaatgaaaa aaattcatcc tctgaggagt cagcggcaat tcgtgtgaca ttggaaaaat
      301 tagctcgctc agaaagaaaa cgccgggcat aaaattctgg catgcggcta ttatgactcc
      361 atccacctaa aagacgaagc tcatctttag cttcactcat cagacctgca atagcgaaat
      421 caactccggc aagcttgcaa tttctcataa cagactgata tttagcagca taaattctct
      481 ttaagatcaa ataagcccat gtatggcgag ttacgtgtgg tgttatgcta acta
//
LOCUS       DAFNBJ010000426          520 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.446, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000426 DAFNBJ010000000
VERSION     DAFNBJ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.446"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>520
                     /locus_tag="L2X49_005112"
     CDS             <1..>520
                     /locus_tag="L2X49_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBN4800027.1"
                     /translation="TAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTN
                     AVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGH
                     LRPLKEASEAENV"
BASE COUNT          142 a          128 c          136 g          114 t
ORIGIN      
        1 gaccgctggc tccctgaagc tgggcaaagt acaggtttca gtgctggtcc gttcattgct
       61 gaaaagtgaa cgtccttccg gactgactca ggcaatcatt gaagtggggc gcatcaacaa
      121 aacgctgtat ctgcttaatt atattgatga tgaagattac cgccggcgca ttctgaccca
      181 gcttaatcgg ggagaaagtc gccatgccgt tgccagagcc atctgtcacg gtcaaaaagg
      241 tgagataaga aaacgatata ccgacggtca ggaagatcaa ctgggcacac tggggctggt
      301 cactaacgcc gtcgtgttat ggaacactat ttatatgcag gcagccctgg atcatctccg
      361 ggcgcagggt gaaacactga atgatgaaga tatcgcacgc ctctccccgc tttgccacgg
      421 acatatcaat atgctcggcc attattcctt cacgctggca gaactggtga ccaaaggaca
      481 tctgagacca ttaaaagagg cgtcagaggc agaaaacgtt
//
LOCUS       DAFNBJ010000427          519 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.447, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000427 DAFNBJ010000000
VERSION     DAFNBJ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.447"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..>519)
                     /locus_tag="L2X49_005113"
     CDS             complement(<1..>519)
                     /locus_tag="L2X49_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003953979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBN4800028.1"
                     /translation="IINGVPVLLVEAKTPGEDLDSAFQEARLYALELNAKYESGMNPL
                     TKIIATDGYEWLAGEWDNNQGSYRLNEISPVDKNCANFLEEFSFTALSKLSEEFIAKS
                     KPRKFWKPRRMMGGKAAQNEEVGHNSFGATLISEYGNIFNPNTLNDRQFIAREGYISS
                     KRRDRYTQPIDKI"
BASE COUNT          144 a          121 c           78 g          176 t
ORIGIN      
        1 aattttatct attggctggg tatagcggtc cctgcgctta gatgaaatat acccttcacg
       61 agcgataaac tgtcgatcat tcagtgtatt tggattaaat atatttccat actctgatat
      121 aagtgttgca ccaaaactat tatgccccac ctcttcgttc tgagctgcct tccctcccat
      181 cattctccta ggcttccaga attttctagg ttttgattta gctatgaatt cctcagataa
      241 tttcgagaga gctgtaaacg aaaattcctc taaaaaattt gcacaatttt tatcaacagg
      301 tgatatctca tttaatcggt aactaccctg gttattatcc cattcccccg caagccactc
      361 atatccatca gtagctatga ttttggtaag aggattcata ccactctcat attttgcatt
      421 caactctagc gcatataatc gtgcttcctg aaaagcgcta tctaaatcct ctccaggagt
      481 ttttgcttct actagtaata caggaacacc atttataat
//
LOCUS       DAFNBJ010000428          509 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.448, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000428 DAFNBJ010000000
VERSION     DAFNBJ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.448"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            172..394
                     /locus_tag="L2X49_005114"
                     /pseudo
     CDS             172..394
                     /locus_tag="L2X49_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          176 a           74 c           86 g          173 t
ORIGIN      
        1 tcctctgcaa atgagcgaaa tttgatagaa catttatctg ctgtacgatt taatgaactt
       61 aatgatacag cattacaaaa acaatcgaag tttataaaga tgattcctga ttgaccagaa
      121 ccctatccta caatggtgac taattttatt actcccgatg gagatgatga tatgaatata
      181 tcatatgtga atagcaacaa aacgacatct ttacctgttg agctagatgc actaaataat
      241 aaagatatct cttatgccaa agatttttct tatgctaaag atttcttttt atacatagaa
      301 acacagttaa aaattgcaaa agatttttgt agacctggag aagaagtatc aagttctatt
      361 gcaagtaaag tttttcacgc atttattgat ttagttaaca aaatcagggg taagaaagac
      421 tttatgtata tttgcacgct ttgctgtttt gccgaggagg ttaaaggtga ttattctcat
      481 tacaggacct ttttatttga tattggtaa
//
LOCUS       DAFNBJ010000429          505 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.449, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000429 DAFNBJ010000000
VERSION     DAFNBJ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.449"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            37..>505
                     /locus_tag="L2X49_005115"
     CDS             37..>505
                     /locus_tag="L2X49_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBN4800029.1"
                     /translation="MDLIMEWRFLGSLSEARKSGCSGVYLIVHKGLFSRVVYVGVSCN
                     VGRRITEHYDGYLRGNRTIYDAGHDEDVYRFMSAYKIHNHTKYYQALANDYKIWASTT
                     MYSDLPKNMLAKSQTFDTDWQSIALEKYIPQLVVWALPMAKYCYLNASRIESVI"
BASE COUNT          171 a           83 c          106 g          145 t
ORIGIN      
        1 cgaaaataat gataaacaaa aacagtagca ataattatgg atttaattat ggaatggaga
       61 tttttaggtt ctctatcaga ggccaggaaa tctggctgta gcggagttta tcttattgtc
      121 cataaaggac ttttcagtcg tgtggtttac gttggggtaa gttgtaatgt aggtagaagg
      181 ataaccgaac attacgacgg ttatcttaga ggaaatcgaa caatttacga cgctggtcac
      241 gacgaagacg tttatcgctt catgtcagct tataaaattc ataaccacac aaaatactat
      301 caagccttgg cgaatgatta taagatatgg gcatctacca caatgtattc tgatttgccg
      361 aaaaacatgc tagcaaaaag ccaaaccttt gatactgatt ggcagagcat tgcattagaa
      421 aaatatattc ctcaactggt agtatgggcc cttccaatgg caaagtactg ttatttaaat
      481 gcaagcagga ttgaaagtgt aatcc
//
LOCUS       DAFNBJ010000430          505 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.450, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000430 DAFNBJ010000000
VERSION     DAFNBJ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.450"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            12..>505
                     /locus_tag="L2X49_005116"
     CDS             12..>505
                     /locus_tag="L2X49_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5B family transposase"
                     /protein_id="HBN4800030.1"
                     /translation="MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
                     APSST"
BASE COUNT          121 a          144 c          126 g          114 t
ORIGIN      
        1 gatatgagat catgtttgtc atctggagcc atggaacagg gttcatcatg agtcatcaac
       61 ttaccttcgc cgacagtgaa ttcagcagta agcgccgtca gaccagaaaa gagattttct
      121 tgtcccgcat ggagcagatt ctgccatggc aaaacatggt ggaagtcatc gagccgtttt
      181 accccaaggc tggtaatggc cggcgacctt atccgctgga aaccatgcta cgcattcact
      241 gcatgcagca ttggtacaac ctgagcgatg gcgcgatgga agatgctctg tacgaaatcg
      301 cctccatgcg tctgtttgcc cggttatccc tggatagcgc cttgcctgac cgcaccacca
      361 tcatgaattt ccgccacctg ctggagcagc atcaactggc ccgccaattg ttcaagacca
      421 tcaatcgctg gctggccgaa gcaggcgtca tgatgactca aggcaccttg gtcgatgcca
      481 ccatcattga ggcacccagc tcgac
//
LOCUS       DAFNBJ010000431          497 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.451, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000431 DAFNBJ010000000
VERSION     DAFNBJ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.451"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..138)
                     /locus_tag="L2X49_005117"
     CDS             complement(<1..138)
                     /locus_tag="L2X49_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HBN4800031.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ED"
     gene            complement(181..417)
                     /locus_tag="L2X49_005118"
     CDS             complement(181..417)
                     /locus_tag="L2X49_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HBN4800032.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          145 a          105 c           90 g          157 t
ORIGIN      
        1 atcttcctca gccgatttca ttacgagtaa ttgcttaata agagtctgca atgaaaaatt
       61 tatcttcatg cgcagtatct ggagcaccgc ctggccagta atttcatatt ttatacgttc
      121 attatcagat gtcttcataa cgacttcata gctaacctgt taaataatca ggctgttgca
      181 ttattcaaaa agcaacccaa ttagtgcatt ataatgtttc tgttcctctt cccctgtcgc
      241 ttgttccagt cggcaaagaa gtttggcaca aagcgacttt cgactcagat tacgtccatc
      301 tttcaaaatc tcagtcacaa tttccccgag agtttcctgc tgagtaggca agttagcttt
      361 tgcaaagtaa cgagtgattg ctcccgcaga atctgaagtc actgaatttt gatgcatgct
      421 acgcctctgt taaaaatgtt aatcaatgtg aagtgaggta acaatagcaa acttgtacga
      481 aatatgtgta cagaatg
//
LOCUS       DAFNBJ010000432          497 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.452, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000432 DAFNBJ010000000
VERSION     DAFNBJ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.452"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..497
                     /gene="espX1"
                     /locus_tag="L2X49_005119"
                     /pseudo
     CDS             1..497
                     /gene="espX1"
                     /locus_tag="L2X49_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
BASE COUNT          190 a           87 c           73 g          147 t
ORIGIN      
        1 cgatcaaaag atccagtatt tgactgcccc taatatcaat atacataaaa tgtcgctatc
       61 aaatataaca atagaaaaga gcaacctttc atatgggtat tattttggtt gtgtattatc
      121 caatatatca tgttttgaaa gtgacttatc aaacatcatc ttttcaaatg gagaaataaa
      181 caacctcttc attaaaaaag caacatattt ggagcatcat tcactaatac cagaataaaa
      241 aattttctct gtgaagatat tatgcctggt cgatggacca ctcaacttgt caataagcac
      301 ttaggttaca gatacacagg agtattcaaa accctggcaa gtattgatga caaaccgtcc
      361 cgtttcgaaa ttttgatacc attggtacaa actttagttc gcgacaatgt aaagctaaat
      421 aatgatgtgt ataaagaact taataagttt atgcacgatt acgacaaaac atcatcagaa
      481 atgagaaaat accttaa
//
LOCUS       DAFNBJ010000433          491 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.453, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000433 DAFNBJ010000000
VERSION     DAFNBJ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.453"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..271)
                     /locus_tag="L2X49_005120"
     CDS             complement(<1..271)
                     /locus_tag="L2X49_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800033.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFK"
BASE COUNT          145 a          100 c           97 g          149 t
ORIGIN      
        1 ctttaaagac ggtatcgaaa tcgccattgg cattgttata gtaaacaaaa ccattgtaag
       61 ttacgtagtt accatctgaa cccatcgggt ccatgccgcc agcatcggca attccatcat
      121 tgtcggtgtc atacggccaa actaaatcgg tcgtggtgtt gtcatgatac ccccaggtat
      181 catagtttgc tgcattagcc tgagaggtga tagccagagt gcaagccaat gctaattgtg
      241 atactacaag tttctttttc caggagtgca tttgtcatcc ctcctcaggg acgtaataag
      301 ttgatccatc aattatcaat gcatagaaaa catattatgt ttcgcgctaa cgattatgct
      361 gcgttacaga cgaatggttc aattattctt tcccctaagt ccgaatgtgg acatatataa
      421 tttatggaga aaaacacgca tcaaattgat aaaatggcga tatttcaaca tgttgcagat
      481 aatgttattt t
//
LOCUS       DAFNBJ010000434          485 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.454, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000434 DAFNBJ010000000
VERSION     DAFNBJ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.454"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..476
                     /locus_tag="L2X49_005121"
     CDS             <1..476
                     /locus_tag="L2X49_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN4800034.1"
                     /translation="FFNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIK
                     TVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYA
                     IPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
BASE COUNT          146 a           91 c          129 g          119 t
ORIGIN      
        1 atttttttaa tgaactgaca gttcaatcag catcagccca aaaaacgcct tattgcgtca
       61 tgattatgga tatcgaccac ttcaaaaaag tcaacgacac ctgggggcat ccggttggcg
      121 atcaggtgat aaaaacagtg gtgaatatca ttgggaaaag catacgacca gatgaccttt
      181 tagcgcgcgt cggcggcgaa gagtttggcg tcttgctgac ggacatcgat actgaacgtg
      241 cgaaagcttt agcggaaagg attcgggaaa atgttgagcg tttaactggc gataatcctg
      301 aatacgctat accacaaaaa gtgacgatta gtattggcgc tgttgtgact caggaaaatg
      361 cgttaaaccc aaatgagatc tatcgactgg ctgataacgc actttacgag gcgaaagaga
      421 ccgggcgtaa taaggtggtt gtgagggatg tggtgaattt ttgtgagtca ccataaagcg
      481 gcatt
//
LOCUS       DAFNBJ010000435          483 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.455, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000435 DAFNBJ010000000
VERSION     DAFNBJ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.455"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
BASE COUNT          137 a          109 c          105 g          132 t
ORIGIN      
        1 atcatcatca gcgtcgatat ggacgaatat aaaaacattt acgttatatc ccaaaaacac
       61 acaggtctta gggaggttta aactgtgtat taacacctat cgtattgacg ggcgtgaaat
      121 ggcagaaaca gaagtcgttc ctattgacat gcctgactct aacggcgaga tgacttggca
      181 agccaaaaac tacacacagt attcgtccta ctttatgaag attacttgct tgaaatagct
      241 tattggcaaa tggcatatac agttattgaa ccattctgat cttcggttgt atcgcacgca
      301 gccgaccatg agttattggc tgatgggaat gacctggtaa gccatatgta accagttcgt
      361 gaggtgcata ttccgccgcc acctgtaaca cgttttccag ttggacaggt agctgttgct
      421 ccaggaaagc gccaacctgt cgcctgtgct gtagcaattt gaagctcacc agatcctacc
      481 ttt
//
LOCUS       DAFNBJ010000436          481 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.456, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000436 DAFNBJ010000000
VERSION     DAFNBJ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.456"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(148..354)
                     /locus_tag="L2X49_005122"
     CDS             complement(148..354)
                     /locus_tag="L2X49_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBN4800035.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          139 a          108 c           90 g          144 t
ORIGIN      
        1 aacaattagg aaaatattta tatatatttc atgaatatat gtttcgattg gtataacgat
       61 gtctaaatat tcctctgaac atcttcctga tacaaaaaac cgccagcgtt tccgctggcg
      121 gttttcaatg tattcaaaac tcagcaatca ataaaaatca ccagttgcct tttcggcctg
      181 atccatccac accggtttat cgctggtttt cgaccagacg cgatgcaggt agctgtaaaa
      241 gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc
      301 ataagtacgg cgtaaaaaga taagccatgc aggatattct ctgtagagtt ccatactttt
      361 ctcccccttt tgtatctacc cggtgaatgg caccggaaaa atgaatttgt ttatctgatg
      421 aaaatagtac cgccttttgt gtaattttac tactcatcag accacttatt tttgcttatt
      481 g
//
LOCUS       DAFNBJ010000437          479 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.457, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000437 DAFNBJ010000000
VERSION     DAFNBJ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.457"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            79..>479
                     /locus_tag="L2X49_005123"
     CDS             79..>479
                     /locus_tag="L2X49_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800036.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFESWRKKLPAISGPVNLYIPTR
                     FKDKYLEVESYFVDKSIWNVHITFHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNH
                     CFSPDKVNEICEQARHYLVEKMFETHSLDMN"
BASE COUNT          170 a           63 c           83 g          163 t
ORIGIN      
        1 aggcattatt gatgtgatgg ataattttat acccttgata agcttgttga gctcagaact
       61 agaaaaaaaa gaataaacat gataaacgat cttaagagta ttttgttaaa atcctcagag
      121 gaagttgatg tttttatcaa aatttttgaa agttggagaa aaaaattacc ggcaatatca
      181 ggtcctgtaa atctctatat acctaccaga ttcaaagata agtatcttga agtagagtca
      241 tattttgtcg acaaatcaat atggaatgtc catattacat ttcatgatga taagcgtttt
      301 gtgtttttta ccgatcaatt tattgctgaa tttagtccgc aggaatttgt ggataattgt
      361 gagcaatatt taattaataa tcattgtttc tcccctgaca aggttaacga aatctgcgaa
      421 caagccagac attatttagt tgaaaaaatg tttgaaacac attctttgga tatgaataa
//
LOCUS       DAFNBJ010000438          475 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.458, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000438 DAFNBJ010000000
VERSION     DAFNBJ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.458"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(5..157)
                     /locus_tag="L2X49_005124"
     CDS             complement(5..157)
                     /locus_tag="L2X49_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800037.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            175..366
                     /locus_tag="L2X49_005125"
     CDS             175..366
                     /locus_tag="L2X49_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBN4800038.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          130 a          116 c           81 g          148 t
ORIGIN      
        1 catgttaatt catcattatt acatcatcat tgtaaataat taaattaact tccataacat
       61 taaaatatgt atccactgac gcttttttac ataacgaaga attgaccatt ttgtcctgtt
      121 gtgccttaat gtaagtaccg tccacagcgt gggacatact tcaaggaacc ttttgtgagt
      181 caggcaacca gtatgcgaaa acgacaccga tttaacagtc gcatgacccg tatcgtactg
      241 ctcatcagct ttatcttctt ctttggccgt tttatctact cgtccgtcgg tgcctggcag
      301 caccatcaga gcaaaaaaga agctcagcaa tccacactct ccgtcgaatc accggtacaa
      361 cgttagcggt taccttctcc actttcacag aacataacgg cacttcgctg tcggatgctt
      421 ttgctgtttg ggattatcaa agcggcagat attctttcat cttaaatttt acgtc
//
LOCUS       DAFNBJ010000439          468 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.459, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000439 DAFNBJ010000000
VERSION     DAFNBJ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.459"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            47..403
                     /locus_tag="L2X49_005126"
     CDS             47..403
                     /locus_tag="L2X49_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBN4800039.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
BASE COUNT          109 a           92 c           96 g          171 t
ORIGIN      
        1 atacccggaa tagactttcg tcacttttaa ttaaagggat gtttttatgc agtggtattt
       61 agccgtatta aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag agtactggat
      121 gtttactctg attaacgcca tcgtcggcgc tattatcaat gtcattcaat tgattttagg
      181 tttagagttt ccattccttt ctctcattta cctggcagca acaatcattc cggtcattgc
      241 tctgtgtgtt cgtcgtctgc acgatactga ccgttcaggt gcgtgggcgc ttctctatct
      301 ggtaccgatc attggctggt tagttctgtt tgtatttgcc tgtttagaag gcaattctgg
      361 cagcaatcgt tacggaaacg atcctaagtt tggttcaaat taatcttaga attggggcga
      421 catttcgccc ctttttatta ataataacaa atattatttt ccgctaaa
//
LOCUS       DAFNBJ010000440          466 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.460, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000440 DAFNBJ010000000
VERSION     DAFNBJ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.460"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..389
                     /gene="wzx"
                     /locus_tag="L2X49_005127"
     CDS             <1..389
                     /gene="wzx"
                     /locus_tag="L2X49_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021536899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O29 family O-antigen flippase"
                     /protein_id="HBN4800040.1"
                     /translation="YLSYVNFTFYKKKTIYVSIGTLLAVAINIALNYILIPIYGIHGA
                     AWATVIAYFMLAFFHYLIATIMLKANPLSLFLLLWYSALLLASYFLVIYLDSLSLWIS
                     LSIKAMIIFIILIILMKTKIHNELKE"
BASE COUNT          134 a           81 c           67 g          184 t
ORIGIN      
        1 tttatttaag ctatgttaac tttactttct ataagaaaaa aacaatatat gtttcaattg
       61 gtacattgct tgcggtagcg ataaatattg ctttgaatta tatactcata ccaatatacg
      121 gtatccacgg agcagcatgg gctacagtaa tagcttattt tatgctggca tttttccatt
      181 atctcatagc aacaataatg ttaaaggcaa acccactctc attgtttctg ttattatggt
      241 attcagcttt gttattggct tcgtatttct tagtaatata ccttgactct ttgtctcttt
      301 ggatctcttt atcaataaag gcaatgatca tttttattat cctcatcatc cttatgaaaa
      361 caaaaatcca taatgaatta aaggaatgac agtgtcgata tatcttctgc ttctagcttt
      421 cgtatttctg cttgcaatgt cagatttttt tatcattgct gatgcc
//
LOCUS       DAFNBJ010000441          461 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.461, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000441 DAFNBJ010000000
VERSION     DAFNBJ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.461"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..450)
                     /locus_tag="L2X49_005128"
     CDS             complement(<1..450)
                     /locus_tag="L2X49_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800041.1"
                     /translation="MSQYLKLGDDQSPVQLDPHSEVGAFKVVGHCAWAKPEDKITPDF
                     LRSRVEPWLTALFQSEHLNILIGAGLSSAIQESATGTKPQGMGWIEDLKVCKAEIDSY
                     VAKTADASGRGKGNIEDQIRSINELIKGLEILTVQNLPVPLPPPGAPP"
BASE COUNT          122 a          103 c           94 g          142 t
ORIGIN      
        1 tgggggagca cctggtggag gtagtggaac cggtaggttt tgtaccgtta aaatctctaa
       61 tcctttaatc aactcattaa ttgaacgaat ctgatcttca atattaccct ttccccttcc
      121 tgaggcgtct gctgttttag ctacataact gtctatttca gccttacaaa ctttgagatc
      181 ttcaatccat cccatacctt gaggtttggt gccggtcgct gactcttgaa tagccgaact
      241 aaggccagcg ccaataagta tatttaagtg ttcagactga aaaagggcag ttaaccaagg
      301 ttctactctt gaacgcaaaa agtcaggagt gattttatct tctggttttg cccatgcgca
      361 gtgtccaacg actttaaatg caccaacttc gctgtgtggg tccaactgaa ccggagattg
      421 gtcatctcca agctttaaat attgactcaa tttgaataca t
//
LOCUS       DAFNBJ010000442          460 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.462, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000442 DAFNBJ010000000
VERSION     DAFNBJ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.462"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..102)
                     /locus_tag="L2X49_005129"
     CDS             complement(<1..102)
                     /locus_tag="L2X49_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HBN4800042.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEP"
BASE COUNT          133 a          109 c          112 g          106 t
ORIGIN      
        1 cggttcgaca agatcaatcc atactgcatt tacaaggggt tgtgactctt cgacttccag
       61 ccgggtcagt cggttatttt ccagttgaaa tgcgctcagc atgaccggga ctcccaatgc
      121 ttaaaatatc ggacagttcg gtgggcacac agaaacaaat tgggtttcag accattaaac
      181 agcctgactc agcgcgacgg gaaaaattga ggtcgctgac aaccgctaag gctatcagca
      241 aaaagggata gccttaggag ttgatcctgg atgacaggat agtgagccag tatctactgg
      301 gtgtgtccaa ggcgaatgtc ctcttagagt gatcgtgcgc gcatgttacg ccagcaaaat
      361 tttgccgtca acacgcaaca gaacaccaca gaacaatagt tgaacttcgc gctttttggt
      421 actcgtcaag gtcgctaaca ggataaaaaa atcccacatc
//
LOCUS       DAFNBJ010000443          458 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.463, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000443 DAFNBJ010000000
VERSION     DAFNBJ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.463"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>458
                     /locus_tag="L2X49_005130"
     CDS             <1..>458
                     /locus_tag="L2X49_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HBN4800043.1"
                     /translation="EMIVLQYNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRE
                     RFAERGFFYKVPAVLRDYLSAIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQP
                     LAMQQRFNLLFPDFVDHIQLPLPLASTLLERITFYAKKNRDELDKISCKW"
BASE COUNT          144 a           80 c           77 g          157 t
ORIGIN      
        1 gaaatgattg ttctgcagta caatctttca gttgaacatt ttaatctgaa tagttcactt
       61 atttactaca taaacaattg gaatttattt ccattgttct gtttattcag tggttatcac
      121 ttttatcgag aaaggtttgc agaacgcgga tttttttata aggttcctgc tgtgctaagg
      181 gattatttat cagctatacc ggtgaaaata aatgaaaagg cacgatacaa acccggcatc
      241 gctagctatc acaatatcat cacatgtggt ttttcaacgt tgtcacccta tataaggcaa
      301 caaccattgg caatgcagca acgatttaat ttattatttc ctgattttgt tgatcacata
      361 caattgccgt taccacttgc atcaactctt ttagagagaa ttacatttta tgcgaaaaaa
      421 aatagagatg agcttgataa aatctcctgc aaatggtg
//
LOCUS       DAFNBJ010000444          437 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.464, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000444 DAFNBJ010000000
VERSION     DAFNBJ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.464"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>437
                     /locus_tag="L2X49_005131"
     CDS             <1..>437
                     /locus_tag="L2X49_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBN4800044.1"
                     /translation="DKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIKT
                     DEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLN
                     KAFEEKFPLKELNNPAHDCSAISEKSHGREEIRLHIVCDVPDEL"
BASE COUNT          150 a           80 c          101 g          106 t
ORIGIN      
        1 gataaagacg tcatcgcaat tgatggaaaa acgctccggc actcttatga caagagtcgc
       61 cgcaagggag cgattcatgt cattagtgcg ttctcaacaa tgcacagtct ggtcatcgga
      121 cagatcaaga cggatgagaa atctaatgag attacagcta tccctgaact tcttaacatg
      181 ttagatatta aaggaaaaat tatcacaact gatgcgatgg gttgccagaa agatattgca
      241 gagaagatac aaaaacaggg aggtgattat ttattcgctg taaaaggaaa ccaggggcgg
      301 cttaataaag cctttgagga aaaatttccg ctgaaagaat taaataatcc agcgcatgac
      361 tgttccgcaa ttagtgaaaa gagtcacggc agagaagaaa tccgtcttca tattgtttgc
      421 gatgtccctg atgaact
//
LOCUS       DAFNBJ010000445          433 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.466, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000445 DAFNBJ010000000
VERSION     DAFNBJ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.466"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>433
                     /locus_tag="L2X49_005132"
     CDS             <1..>433
                     /locus_tag="L2X49_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HBN4800045.1"
                     /translation="NASGLFESFSRKAAGLLKEVPVVKNFSTTLEAMSFLTVSKMIVV
                     IDDLERRGKNLDVKDVLGLVSLLKEQKDCKVVLLLNNGTASMEDYSTYKEKVIDRDIT
                     YNPTPEECADIAYKGDSHVYNLLSKYSISLGIKNIRILKKIE"
BASE COUNT          154 a           60 c           83 g          136 t
ORIGIN      
        1 aacgcaagtg gactttttga atcattttct cgaaaagcag caggtttgtt aaaagaagtt
       61 cctgttgtaa aaaacttctc tacgacactt gaagcaatgt catttctgac cgtttctaaa
      121 atgattgtag taatagatga cttagaaagg cgtggcaaaa atttagatgt taaagatgtt
      181 ttagggctag tatctctatt aaaagagcag aaggattgta aggtggttct attgcttaac
      241 aatggcacag ctagtatgga ggattattct acttataaag aaaaggtcat tgatagagat
      301 atcacttaca atccaacacc agaagaatgc gcagatatag cctataaagg tgattctcat
      361 gtttataact tgttaagtaa atattccatt tctttaggta tcaagaatat tcgaatctta
      421 aaaaagatag aac
//
LOCUS       DAFNBJ010000446          419 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.467, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000446 DAFNBJ010000000
VERSION     DAFNBJ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.467"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>419
                     /gene="tuf"
                     /locus_tag="L2X49_005133"
     CDS             <1..>419
                     /gene="tuf"
                     /locus_tag="L2X49_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBN4800046.1"
                     /translation="APEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGA
                     AQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEV
                     RELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILEL"
BASE COUNT           99 a          114 c          119 g           87 t
ORIGIN      
        1 gcgccggaag aaaaagctcg tggtatcacc atcaacactt ctcacgttga atacgacacc
       61 ccgacccgtc actacgcaca cgtagactgc ccggggcacg ccgactatgt taaaaacatg
      121 atcaccggtg ctgctcagat ggacggcgcg atcctggtag ttgctgcgac tgacggcccg
      181 atgccgcaga ctcgtgagca catcctgctg ggtcgtcagg taggcgttcc gtacatcatc
      241 gtgttcctga acaaatgcga catggttgat gacgaagagc tgctggaact ggttgaaatg
      301 gaagttcgtg aacttctgtc tcagtacgac ttcccgggcg acgacactcc gatcgttcgt
      361 ggttctgctc tgaaagcgct ggaaggcgac gcagagtggg aagcgaaaat cctggaact
//
LOCUS       DAFNBJ010000447          418 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.468, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000447 DAFNBJ010000000
VERSION     DAFNBJ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.468"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            36..233
                     /locus_tag="L2X49_005134"
     CDS             36..233
                     /locus_tag="L2X49_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800047.1"
                     /translation="MDGESFNFDDNDIEFIASMYASCKLSGSIAPIMHIMVSIPKNKK
                     KQFYERVEHYCSLYSNKNPPS"
BASE COUNT          151 a           83 c           64 g          120 t
ORIGIN      
        1 cagcatcagt tatgccaaac acataaggaa ttgaaatgga cggagaaagc tttaactttg
       61 atgacaatga tattgaattt attgcttcaa tgtatgcatc atgtaaatta tcaggaagta
      121 tcgcccccat aatgcatata atggttagca ttccaaaaaa taagaaaaaa caattctacg
      181 aacgagttga gcattattgt agtttatatt ccaataaaaa cccaccttct taaattcgct
      241 gcattctagt ggattccgtt aagaaaacaa tgacatacac tcaaacacat cttttttatc
      301 aacagtgaat ttcagttcgg ccagttttta ccagctatta taaaaatagc acccatataa
      361 agtttttacc ccacaggtac agctggccat tcgataaccg cactaaggaa gtgccaac
//
LOCUS       DAFNBJ010000448          414 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.469, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000448 DAFNBJ010000000
VERSION     DAFNBJ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.469"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            8..>414
                     /locus_tag="L2X49_005135"
     CDS             8..>414
                     /locus_tag="L2X49_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBN4800048.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMHSRQHETGGDAIYCGLYGHHIKNGVTYSP"
BASE COUNT          142 a           69 c           79 g          124 t
ORIGIN      
        1 gaacacgatg agcaatgatt accctttagt atccataata ataccgacgt ataattcatc
       61 tgattacatt actgaaactc taacgaaatt agaaaaacaa acttacccaa attttgaaat
      121 tgttattgtt aatgatggtt ctaaagataa cacatcaaac gttttgagag agtatgggtt
      181 aacccactct cgattaatta ttatcaataa agaaaatggc ggtgtttcgt ctgccaggaa
      241 tacaggtatc cgcaaggcgc aaggacagtt tatatgtttt atggatgatg atgatgagat
      301 agatcctaac tatctgctga agatgcattc cagacaacat gagacgggag gagatgccat
      361 ttattgtggg ctttatggcc atcatataaa aaatggtgtt acttactcac ctat
//
LOCUS       DAFNBJ010000449          410 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.470, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000449 DAFNBJ010000000
VERSION     DAFNBJ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.470"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..>410)
                     /locus_tag="L2X49_005136"
     CDS             complement(<1..>410)
                     /locus_tag="L2X49_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBN4800049.1"
                     /translation="SKSPGTIYRVHRAWLDAIEYVSKQGIFNFKLLSSFLEKESLLPT
                     YFFCIVFGLKILCVDEFQGFTLDDYEDLELIPRPNSNDWNVYQNIDNILDPFEKNMIS
                     KGLFEMAAAISRGRKYNLVDLRNATVLGLSYVTG"
BASE COUNT          136 a           81 c           73 g          120 t
ORIGIN      
        1 ctacctgtga cataacttag ccccagaaca gtggcattgc gaaggtccac cagattgtat
       61 tttctgcctc gtgagatagc ggcagccatt tcaaaaagac ctttgctgat catgttcttt
      121 tcaaatgggt caaggatatt atctatgttt tgatatacat tccaatcatt tgaattaggg
      181 cgagggatca attcaaggtc ttcgtagtca tctaaagtaa aaccctgaaa ttcatctaca
      241 caaagaattt tcagaccaaa aacaatacag aaaaaataag tcggcaataa gctttctttt
      301 tcaaggaaag agcttaacaa tttaaagtta aatattcctt gcttcgaaac atattcaatt
      361 gcgtctagcc atgcgcgatg cacacgataa atagtcccag gagattttga
//
LOCUS       DAFNBJ010000450          406 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.471, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000450 DAFNBJ010000000
VERSION     DAFNBJ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.471"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>406
                     /locus_tag="L2X49_005137"
     CDS             <1..>406
                     /locus_tag="L2X49_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN4800050.1"
                     /translation="LQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELL
                     DYVSDITTVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHN
                     EKIEEARICIDKSLQLEPRRRKAVVIKECVDMY"
BASE COUNT          149 a           69 c           75 g          113 t
ORIGIN      
        1 attacaaaat aaaagtgata tacagacgct aaaaacagaa tgttattgcc ttctagcgga
       61 atgccatatg tctttggcac ttcatggaaa aagtgaactt gaacttgctg ctcaaaaagc
      121 attagagctt ttagattatg tatcagacat aaccactgtc gatggaaaaa ttttagctat
      181 tatgggactg ataactggtc tgtctggaca agcaaaagta tctcatatct tatttgaaca
      241 ggctaagata cactcaactg atatagcctc tctctactac tatagggcac tagtccactt
      301 tcataatgaa aaaattgaag aggcaaggat ttgtatagac aaatcactac aactcgaacc
      361 cagaagacga aaagcagttg tgataaaaga atgtgtagat atgtat
//
LOCUS       DAFNBJ010000451          402 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.472, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000451 DAFNBJ010000000
VERSION     DAFNBJ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.472"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..305)
                     /locus_tag="L2X49_005138"
     CDS             complement(<1..305)
                     /locus_tag="L2X49_005138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800051.1"
                     /translation="MEIVIYITISILILDIALIKQRMLANIETSNPYHAQYILRRRDD
                     YLVLFDRDALSCRFFYDAFPGMRLRHQVTGDTDDDRLAHSPADRMYTGKDHQKYTFCT
                     "
BASE COUNT           96 a           95 c          107 g          104 t
ORIGIN      
        1 gtacaaaaag tgtacttctg atgatctttc cctgtataca tccggtctgc ggggctgtgt
       61 gccaggcggt catcgtcggt atcgccggtc acctggtggc gcagacgcat tcccggaaag
      121 gcgtcataaa agaagcggca gctcagggca tcacggtcga acagcaccag gtaatcatcg
      181 cggcggcgca ggatgtactg ggcgtgatac gggttactgg tttcgatatt ggctaacatc
      241 cgctgtttta ttaatgcgat atctaaaatc aggatggaaa tagtgatgta tatcactatt
      301 tccatgatct tgccttgcgt tcatccaggc aggaatatcc ctgttagcag cctctgtgcg
      361 atgtacgcca gcggtctgta aagaaaccga ttaccttata ac
//
LOCUS       DAFNBJ010000452          399 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.473, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000452 DAFNBJ010000000
VERSION     DAFNBJ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.473"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..399
                     /locus_tag="L2X49_005139"
                     /pseudo
     CDS             1..399
                     /locus_tag="L2X49_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT           97 a          103 c          113 g           86 t
ORIGIN      
        1 gtgtagcagc cagccagtta tttctctggc gtaagcaata ccaggaagga agtcttactg
       61 ctgtcgccgc cggagaacag gttgttcctg cctctgaact tgctgccgcc atgaagcaga
      121 ttaaagaact ccagcgcctg ctcggcaaga aaacgatgga aaatgaactc ctcaaagaag
      181 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
      241 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      301 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      361 gcttctccgt atacaccatg ttatcggaga gctgcccac
//
LOCUS       DAFNBJ010000453          394 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.474, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000453 DAFNBJ010000000
VERSION     DAFNBJ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.474"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..>394)
                     /locus_tag="L2X49_005140"
     CDS             complement(<1..>394)
                     /locus_tag="L2X49_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800052.1"
                     /translation="SRCNILQNNKLQPEEVYKESQQTAKLEIFCDEFLKISQSRYGLS
                     TSADSIANLLTFFTKASDAIDRIKTQKIDVHSYGFVPIRHFVEHVITYKNEFAADYEP
                     YTLTFTRGENNEGVLSIESKEGSISQRT"
BASE COUNT          110 a           66 c           70 g          148 t
ORIGIN      
        1 attgttcttt gtgaaataga cccttctttg ctttcgatac tcaaaacacc ttcgttgttt
       61 tcacctcggg taaaggtaag ggtatagggt tcataatccg ctgcaaattc atttttatac
      121 gttatgacat gttcgacaaa atgtcttatt ggtacgaaac cataactgtg aacgtcaatt
      181 ttttgggtct tgattctatc aattgcatct gaggctttcg tgaaaaaagt caataaatta
      241 gctatgctgt ctgctgaggt tgataacccg tatctacttt gagatatttt tagaaactca
      301 tcacaaaaaa tctcaagttt cgcggtttgt tgtgattctt tgtaaacttc ttctggttgc
      361 aatttattat tttgtaaaat attacagcga gata
//
LOCUS       DAFNBJ010000454          392 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.475, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000454 DAFNBJ010000000
VERSION     DAFNBJ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.475"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            151..226
                     /locus_tag="L2X49_005141"
     tRNA            151..226
                     /locus_tag="L2X49_005141"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:185..187,aa:Glu,seq:ttc)
BASE COUNT          110 a           86 c           94 g          102 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaagt gaatagcaag gcgtttacgc gttgggagtg
       61 aggctgaaga gaataaggcc gttcgctttc tattaatgaa agctcaccct acacgaaaat
      121 atcacgcaac gcgtgataag caattttcgt gtccccttcg tctagaggcc caggacaccg
      181 ccctttcacg gcggtaacag gggttcgaat cccctagggg acgccacttg ctggtttgtg
      241 agtgaaagtc gccgacctta atatctcaaa actcatcttc gggtgatgtt tgagatattt
      301 gctctttaaa aatctggatc aagctgaaaa ttgaaacact gaacaacgag agttgttcgt
      361 gagtctctca aattttcgca acacgatgat ga
//
LOCUS       DAFNBJ010000455          376 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.476, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000455 DAFNBJ010000000
VERSION     DAFNBJ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.476"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..>376
                     /locus_tag="L2X49_005142"
     CDS             <1..>376
                     /locus_tag="L2X49_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800053.1"
                     /translation="FILTVSSDFLQLVPKEHRERVITIGNATLSQCAPLYEVCDGLFM
                     PTLLECFSASYLEAMYMKKIIFTSDLPFAHTVCKDAAFYFAPHDVENIRSTLVNGFQN
                     KEILNHKLNEGSKIYESFPSAKA"
BASE COUNT          118 a           65 c           64 g          129 t
ORIGIN      
        1 tttattttaa cagtttcaag tgattttttg cagctagtcc caaaagagca tagagagcga
       61 gtcatcacca taggtaatgc aacacttagt caatgtgctc cgttgtatga agtttgtgat
      121 ggattattca tgccaacact tttagaatgc tttagtgctt cttacttaga agcaatgtac
      181 atgaaaaaaa taatcttcac ttctgatctg ccttttgctc acactgtttg caaagatgca
      241 gcattttact ttgctcctca tgatgttgaa aatattagga gtacacttgt taatggcttt
      301 caaaacaaag aaattcttaa ccataaatta aatgaaggtt cgaaaattta tgaaagtttt
      361 ccttctgcga aagctc
//
LOCUS       DAFNBJ010000456          372 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.denovo.477, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000456 DAFNBJ010000000
VERSION     DAFNBJ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.denovo.477"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            218..325
                     /locus_tag="L2X49_005143"
     CDS             218..325
                     /locus_tag="L2X49_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBN4800054.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           91 a           91 c          112 g           78 t
ORIGIN      
        1 cggaacagaa aaacagtcgt agaatcagcg tcaagccgga agggttccgg tgaggcgcaa
       61 tgttgcgggg gctttatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt
      121 tgcaggtatg cacctgacaa catcacgggg gccattcttg aacctgaaca actcaagaat
      181 agccgcgaaa cgttgtcatt acaacacagg cggctatatg acgctcgcag aactgggcat
      241 ggccttctgg catgatttag cggctccggt cattgctggc attcttgcca gtatgatcgt
      301 gaactggctg aacaagcgga agtaacgtgt catgcgggcg tcaggctgcc gtaatggcaa
      361 tttgcgcccg ga
//
LOCUS       DAFNBJ010000457         4460 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.255, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000457 DAFNBJ010000000
VERSION     DAFNBJ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.255"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            35..382
                     /locus_tag="L2X49_005144"
     CDS             35..382
                     /locus_tag="L2X49_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HBN4800055.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            376..1215
                     /gene="sul1"
                     /locus_tag="L2X49_005145"
     CDS             376..1215
                     /gene="sul1"
                     /locus_tag="L2X49_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HBN4800056.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            1620..3161
                     /locus_tag="L2X49_005146"
     CDS             1620..3161
                     /locus_tag="L2X49_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBN4800057.1"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPA
                     VSEKRLALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVF
                     APNSKHRVQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVC
                     EHCGGHVKVIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQ
                     SRLFD"
     gene            3395..4234
                     /locus_tag="L2X49_005147"
                     /pseudo
     CDS             3395..4234
                     /locus_tag="L2X49_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         1042 a         1239 c         1222 g          957 t
ORIGIN      
        1 cataataagc cctacacaaa ttgggagata tatcatgaaa ggctggcttt ttcttgttat
       61 cgcaatagtt ggcgaagtaa tcgcaacatc cgcattaaaa tctagcgagg gctttactaa
      121 gcttgcccct tccgccgttg tcataatcgg ttatggcatc gcattttatt ttctttctct
      181 ggttctgaaa tccatccctg tcggtgttgc ttatgcagtc tggtcgggac tcggcgtcgt
      241 cataattaca gccattgcct ggttgcttca tgggcaaaag cttgatgcgt ggggctttgt
      301 aggtatgggg ctcataattg ctgccttttt gctcgcccga tccccatcgt ggaagtcgct
      361 gcggaggccg acgccatggt gacggtgttc ggcattctga atctcaccga ggactccttc
      421 ttcgatgaga gccggcggct agaccccgcc ggcgctgtca ccgcggcgat cgaaatgctg
      481 cgagtcggat cagacgtcgt ggatgtcgga ccggccgcca gccatccgga cgcgaggcct
      541 gtatcgccgg ccgatgagat cagacgtatt gcgccgctct tagacgccct gtccgatcag
      601 atgcaccgtg tttcaatcga cagcttccaa ccggaaaccc agcgctatgc gctcaagcgc
      661 ggcgtgggct acctgaacga tatccaagga tttcctgacc ctgcgctcta tcccgatatt
      721 gctgaggcgg actgcaggct ggtggttatg cactcagcgc agcgggatgg catcgccacc
      781 cgcaccggtc accttcgacc cgaagacgcg ctcgacgaga ttgtgcggtt cttcgaggcg
      841 cgggtttccg ccttgcgacg gagcggggtc gctgccgacc ggctcatcct cgatccgggg
      901 atgggatttt tcttgagccc cgcaccggaa acatcgctgc acgtgctgtc gaaccttcaa
      961 aagctgaagt cggcgttggg gcttccgcta ttggtctcgg tgtcgcggaa atccttcttg
     1021 ggcgccaccg ttggccttcc tgtaaaggat ctgggtccag cgagccttgc ggcggaactt
     1081 cacgcgatcg gcaatggcgc tgactacgtc cgcacccacg cgcctggaga tctgcgaagc
     1141 gcaatcacct tctcggaaac cctcgcgaaa tttcgcagtc gcgacgccag agaccgaggg
     1201 ttagatcatg cctagcattc accttccggc cgcccgctaa atatctcctt ttgggttgtt
     1261 aataaaacat ccaataagtt gactgtgcgt gaaaaagaaa gttttgtgtg atggcgttga
     1321 agatcgcacc gttaagctct tatgtgggat ggtgcagagc tcgacgacta ccgataaaac
     1381 gcaaccgccg caaacagaca agaaaaagcc ccaactgata acagttgggg cttcagtatt
     1441 gtgattggtg gagcaatagc accctgaacc caaaaccttc tcgctcaacc ggtagtggct
     1501 gataacaact cgtgagggct attgcgggtt aagcatttag cgatgtctag ggccagactg
     1561 gacgtctgaa cgcaagccgc tgatactgta cataaccaca gtatcagcgg aggataccca
     1621 tgtcgctggc aaggaacgcc acggcgagtc aatcgcccac tcaaacaaac ggttacgaac
     1681 gccaccaacc cgaccagacg ctgctctacc agctggttga gcagcactac ccagccttca
     1741 aagcctcact cgaagcccaa ggtcaacacc tgcctcgcta catccaacaa gaattcaacg
     1801 acctcctcca atgtggccgt ctggagtatg gtttcatgcg ggttcgctgc gaggattgtc
     1861 atcacgagcg tctggtcgcc ttcagctgta aacgacgcgg cttttgccct agctgcggtg
     1921 cccgccggat ggccgagagt gcggcgctgc tgatagacga agtcttcccc aaggagccca
     1981 ttcgccagtg ggtgctcagc tttcctttcc agctacgctt tttgctggct cgccatcccc
     2041 agctgatggg ccaggtcttg agtatcgtct atcgtacact ctcaactcat ctgatcaaaa
     2101 aagccggtta caccaaagcc tctgcacaaa ctggctcagt gactcttatc caacgctttg
     2161 gctccgcgct aaatctcaat gtccactacc acatgctgtt tctcgatggt gtctatgccg
     2221 aagatgacta tggcaagcaa cgcttccatc gtgtcaaggc acccacttac gatgagctga
     2281 atacgctcgc tcacaccctc agccatcgca tcgctcgctg catggaaaag cgtgggattt
     2341 tggagcgtga tgccgagaat acgtggttga cactggaaga gggcgaagac gatacgctga
     2401 ctcaattaca tggtgcttcg gttacgtatc gcattgccgt cggcccccag caagggcgca
     2461 aagtcttcac cctgcaaacc ttgccagggc gtgaggataa agccgactca agcagtcgag
     2521 tagccaacca tgctggtttc tcgctacacg ccggtgtgat ggccgaagcg catcagcggg
     2581 ataagcttga gcgcttgtgt cgctacatta gtcggccagc ggtttcagaa aaacgtctgg
     2641 cattaaccgc caatgggcag gtgcgttacg agctcaaaac tccgtaccgc aatggcacca
     2701 cccatgtgat cttcgagccg ctggacttca tcgccaaact cgctgcgttg gtacctaagc
     2761 cgcgagtcaa cctcacacgc ttccacggcg tctttgcacc gaacagcaaa caccgagttc
     2821 aagtaacacc cgccaagcgg ggcaagaagc ccgacaaatc ggaaggtctc gatactaatt
     2881 ggcgtgacaa gagtcctgca gagcgccacc gcgccatgac ctggatgcaa cgcctcaagc
     2941 gagtcttcaa tattgatatt gaagtctgcg aacactgcgg cggtcacgtc aaagtgattg
     3001 ccagcatcga agatccgaag gtcattgagc agattctcaa gcatctgaaa cagaaaacag
     3061 ccaaggcgaa tgccgccaag cagcgtgagc tgccaccaga acgagcgccg ccactgactc
     3121 ccagcctgtt cgatccatca cagagtcgtc tctttgactg acgaccccaa atccaacact
     3181 gctcaacact gccaactttt aaacggggcg gtggggcagt ttgtatctct cgagctatca
     3241 ggctagagat tttaccgcca aatcgaacct tattagagcg gtttaggctg gaccggcagt
     3301 taaaattggg gcttgagcgg taaacgagtg agggaatttc aggtaagata cttcggatga
     3361 ggagcaaaaa ggtggtttat acttcctata cccctggatg gacgaacaag ccattaagca
     3421 gtggcattgc ctggtccacc atggccgccg agggagaggt ttccactaca gtgacaccgc
     3481 cattgagacg gccctgatgc taaaaggctt gttcaagttg ccgctgcggg cgctagaagg
     3541 cttcattaac tcgttgttcc aactgatggc ggtgcctttg cagtcgccgg actatagctg
     3601 tatcagcaag cgcgccaaga ccgtcgacat caagtaccgc ctccccagcc aaggcccggt
     3661 ggctcacttg gtcattgatg ccaccggcct caaggtctat ggcgaaggcg aatggaaaat
     3721 tcgcaaacac ggcaaggaga agcgacgagt gtggcgcaag ctccacttgg cggtggatgc
     3781 cacaacccat gcggtcatcg ctgccgaagt cagcctggag acggtggcgg ataacgaggt
     3841 gttaccgacg ttgctcaacc ccttacgacg caagataaaa caggtcagcg ccgatggtgc
     3901 gtatgacacc aaggcctgtc atgcgctact gaagaaaaag ggggcgaaag ccaccatacc
     3961 gccgagaaaa aatgcggcac cgtgggagga aggccatccc cgcaacgagg cggtgacggc
     4021 gctcaaggcc ggtgaactga agcaatggaa gaaagactcc ggctatcacc agcgctcgat
     4081 agccgagacc gccatgtacc ggttcaagca actcatcggg ccaacgctga gcctgcggaa
     4141 ctacaacgcc caggtgggcg aaatcctggc tggcgtgaag gtgatgaaca agctgatagg
     4201 gcttggtatg cctgttcgcc agccagtgaa ttgagtggta tcaacgggtt ggggaacggc
     4261 catccagcga cggatttggt caacaacgcc gataacacct cgaaatcgga gtttatatgg
     4321 cttcgtgggg gagttatgtg tttgagcttt gtcgcattaa cgcgaatcag gggttgacgg
     4381 cagaaatgga ctggaattac acttcttaga gtcggcgttg ccggaaaatt ctgattggat
     4441 tagttgttcg gggtgcgaaa
//
LOCUS       DAFNBJ010000458         2841 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.295, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000458 DAFNBJ010000000
VERSION     DAFNBJ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.295"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(41..907)
                     /gene="intI1"
                     /locus_tag="L2X49_005148"
                     /pseudo
     CDS             complement(41..907)
                     /gene="intI1"
                     /locus_tag="L2X49_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
     gene            1052..1549
                     /gene="dfrA12"
                     /locus_tag="L2X49_005149"
     CDS             1052..1549
                     /gene="dfrA12"
                     /locus_tag="L2X49_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /protein_id="HBN4800058.1"
                     /translation="MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGK
                     VVVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVA
                     GGAEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELVSTETIQAVIPYTHSVY
                     ARRNG"
     gene            1661..1951
                     /locus_tag="L2X49_005150"
     CDS             1661..1951
                     /locus_tag="L2X49_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1010 domain-containing protein"
                     /protein_id="HBN4800059.1"
                     /translation="MFIQTAFSFSGVIQCLFCLFSGLRLHGLRRFSVFLASSPCVASA
                     SSYRFCSAVPPRWRSVFSRLAPVAKFKLSVLASGSNISVKPTRILRSAYLAR"
     gene            1957..2748
                     /locus_tag="L2X49_005151"
     CDS             1957..2748
                     /locus_tag="L2X49_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /protein_id="HBN4800060.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQ
                     DLFEALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
BASE COUNT          634 a          793 c          765 g          649 t
ORIGIN      
        1 acaaaaggct gcctcatcgc taactttgca acagtgccct gcggagcaag gccgtcgcga
       61 acgagtggcg gagggtgtgc ggtgtggcgg gcttcgtgat gcctgcttgt tctacggcac
      121 gtttgaaggc gcgctgaaag gtctggtcat acatgtgatg gcgacgcacg acaccgctcc
      181 gtggatcggt cgaatgcgtg tgctgcgcaa aaacccagaa ccacggccag gaatgcccgg
      241 cgcgcggata cttccgctca agggcgtcgg gaagcgcaac gccgctgcgg ccctcggcct
      301 ggtccttcag ccaccatgcc cgtgcacgcg acagctgctc gcgcaggctg ggtgccaagc
      361 tctcgggtaa catcaaggcc cgatccttgg agcccttgcc ctcccgcacg atgatcgtgc
      421 cgtgatcgaa atccagatcc ttgacccgca gttgcaaacc ctcactgatc cgcatgcccg
      481 ttccatacag aagctgggcg aacaaacgat gctcgccttc cagaaaaccg aggatgcgaa
      541 ccacttcatc cggggtcagc accaccggca agcgccgcga cggccgaggt cttccgatct
      601 cctgaagcca gggcagatcc gtgcacagca ccttgccgta gaagaacagc aaggccgcca
      661 atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg
      721 cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
      781 cacgaaccca gtggacataa gcctgttcgg ttggtaagct gtaatgcaag tagcgtatgc
      841 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
      901 ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca tccaagcagc
      961 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     1021 agggcagtcg ccctaaaaca aagttagcca tatgaactcg gaatcagtac gcatttatct
     1081 cgttgctgcg atgggagcca atcgggttat tggcaatggt cctaatatcc cctggaaaat
     1141 tccgggtgag cagaagattt ttcgcagact cactgaggga aaagtcgttg tcatggggcg
     1201 aaagaccttt gagtctatcg gcaagcctct accgaaccgt cacacattgg taatctcacg
     1261 ccaagctaac taccgcgcca ctggctgcgt agttgtttca acgctgtcgc acgctatcgc
     1321 tttggcatcc gaactcggca atgaactcta cgtcgcgggc ggagctgaga tatacactct
     1381 ggcactacct cacgcccacg gcgtgtttct atctgaggta catcaaacct tcgagggtga
     1441 cgccttcttc ccaatgctca acgaaacaga attcgagctt gtctcaaccg aaaccattca
     1501 agctgtaatt ccgtacaccc actccgttta tgcgcgtcga aacggctaac cattccgtca
     1561 acgggacgcc aaaatgctgc gcattttggt tccctccgct gcgctccggc tctcgttacg
     1621 tccaacgtta gcaccactga aacccagctt tatttagctc atgtttattc aaacggcatt
     1681 tagcttttca ggcgttattc agtgcctgtt ttgccttttt tccgggcttc gcctgcatgg
     1741 gctgcgcagg ttttcagtct ttttggcctc tagcccttgc gtagcaagcg caagcagcta
     1801 tcgtttttgc agtgctgtgc cgcctcggtg gcgcagcgtt ttttcacggt tagcgcccgt
     1861 cgccaaattc aagttatccg ttttggcttc tggttctaac atttcggtca agccgacccg
     1921 cattctgcgg tcggcttacc tcgcccgtta gacatcatga gggaagcggt gaccatcgaa
     1981 atttcgaacc aactatcaga ggtgctaagc gtcattgagc gccatctgga atcaacgttg
     2041 ctggccgtgc atttgtacgg ctccgcagtg gatggcggcc tgaagccata cagcgatatt
     2101 gatttgttgg ttactgtggc cgtaaagctt gatgaaacga cgcggcgagc attgctcaat
     2161 gatcttatgg aggcttcggc tttccctggc gagagcgaga cgctccgcgc tatagaagtc
     2221 acccttgtcg tgcatgacga catcatcccg tggcgttatc cggctaagcg cgagctgcaa
     2281 tttggagaat ggcagcgcaa tgacattctt gcgggtatct tcgagccagc catgatcgac
     2341 attgatctag ctatcctgct tacaaaagca agagaacata gcgttgcctt ggtaggtccg
     2401 gcagcggagg aattctttga cccggttcct gaacaggatc tattcgaggc gctgagggaa
     2461 accttgaagc tatggaactc gcagcccgac tgggccggcg atgagcgaaa tgtagtgctt
     2521 acgttgtccc gcatttggta cagcgcaata accggcaaaa tcgcgccgaa ggatgtcgct
     2581 gccgactggg caataaaacg cctacctgcc cagtatcagc ccgtcttact tgaagctaag
     2641 caagcttatc tgggacaaaa agaagatcac ttggcctcac gcgcagatca cttggaagaa
     2701 tttattcgct ttgtgaaagg cgagatcatc aagtcagttg gtaaatgatg tctaacaatt
     2761 cgttcaagcc gaccgcgcta cgcgcggcgg cttaactccg gcgttagatg cactaagcac
     2821 ataattgctc acagccaaac t
//
LOCUS       DAFNBJ010000459         2807 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.297, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000459 DAFNBJ010000000
VERSION     DAFNBJ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.297"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..273
                     /locus_tag="L2X49_005152"
     CDS             <1..273
                     /locus_tag="L2X49_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HBN4800061.1"
                     /translation="ELIFGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTK
                     TWFELAPKAANIIVKDEKMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            284..643
                     /gene="frdD"
                     /locus_tag="L2X49_005153"
     CDS             284..643
                     /gene="frdD"
                     /locus_tag="L2X49_005153"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBN4800062.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            706..1839
                     /locus_tag="L2X49_005154"
     CDS             706..1839
                     /locus_tag="L2X49_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBN4800063.1"
                     /translation="MFKTTLCALLITASCSTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNALQ"
     gene            1928..2461
                     /gene="blc"
                     /locus_tag="L2X49_005155"
     CDS             1928..2461
                     /gene="blc"
                     /locus_tag="L2X49_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBN4800064.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2458..2775)
                     /gene="sugE"
                     /locus_tag="L2X49_005156"
     CDS             complement(2458..2775)
                     /gene="sugE"
                     /locus_tag="L2X49_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBN4800065.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT          694 a          735 c          718 g          660 t
ORIGIN      
        1 gaactgattt tcgggctgtt tgccctgaaa aatggcccgg aagcctgggc gggattcgtc
       61 gactttttac aaaacccggt tattgtgatc attaacctga tcactctgac ggcagctctg
      121 ctgcacacca aaacctggtt tgaactggca ccgaaagcgg ccaatatcat tgtaaaagac
      181 gaaaaaatgg gtccagagcc aattatcaaa agtctctggg cggtaactgt ggttgccacc
      241 atcgtaatcc tgtttgttgc cctgtactgg taaggagcct gagatgatca atccaaatcc
      301 aaagcgttct gacgaaccgg tattctgggg cctcttcggg gccggtggta tgtggagcgc
      361 aattattgcg ccggtgatga tcctgctggt gggtattctg ctgccgctgg ggctgtttcc
      421 gggtgatgca ctgagctacg agcgcgttct ggcgttcgcg cagagcttca ttggtcgcgt
      481 attcctgttc ctgatgatcg ttctgccgct gtggtgtggt ttacaccgta tgcaccacgc
      541 gatgcacgat ctgaaaatcc acgtacctgc gggcaaatgg gttttctacg gtctggctgc
      601 tatcctgaca gttgtcacgc tgattggtgt cgttacaatc taacgcatcg ccaatgtaaa
      661 tccggcccgc ctatggcggg ccgttttgta tggaaaccag accctatgtt caaaacgacg
      721 ctctgcgcct tattaattac cgcctcttgc tccacatttg ctgcccccca aaaaatcaac
      781 gatattgtgc atcgcacaat taccccgctt atagagcaac aaaagatccc cggtatggcg
      841 gtggcggtaa tttatcaggg taaaccttat tactttacct ggggctatgc ggacatcgcc
      901 aaaaagcagc ccgtcacaca gcaaacgttg tttgagttag gttcggtcag caaaacattt
      961 acgggcgtgc ttggtggcga cgctattgct cgaggggaaa tcaagttaag cgatcccgca
     1021 acaaaatact ggcctgaact taccgctaaa cagtggaacg ggatcacact attacatctc
     1081 gcaacctaca ccgctggcgg cctgccattg caggtgccgg atgaggtgaa atcctcaagc
     1141 gacttgctgc gcttctatca aaactggcag cctgcatggg ctccaggaac acaacgtctg
     1201 tatgccaact ccagtatcgg tttgttcggc gcactggctg tgaagccgtc tggtttgagt
     1261 tttgagcagg cgatgcaaac acgtgtcttc cagccactca aactcaacca tacgtggatt
     1321 aatgtaccgc ccgcagaaga aaagaattac gcctggggat atcgcgaagg caaggcagtt
     1381 catgtttcgc caggggcgtt agatgctgaa acttatggtg tgaagtcgac cattgaagat
     1441 atggcctgct gggtacgaag caatatgaat ccccgtgata tcaacgacaa aacacttcag
     1501 caagggatac aactggcaca atctcgctac tggcaaaccg gcgatatgta tcagggcctg
     1561 ggctgggaaa tgctggactg gccggtaaat cctgacagca tcattaacgg cagtggcaat
     1621 aaaattgcac tggcagcacg ccccgtaaaa gcgattacgc ccccaactcc tgcagtacgc
     1681 gcatcatggg tacataaaac aggggcaacc ggcggatttg gtagctatgt cgcgtttatt
     1741 ccagaaaaag agctgggtat agtgatgctg gctaacaaaa actatcccaa tccagcgaga
     1801 gtcaccgccg cctggcagat tctcaacgct ctacagtaaa atttcatcgg gtccgaattt
     1861 tcggaccttt tctgcgcttt tccttgctgt catctacact tagaaaaaaa ccagtaagga
     1921 aacatttatg cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc
     1981 ctgcagttct cctacgccgc cgcgtggcgt gacagtagtg aataatttcg acgctaaacg
     2041 ctatctcggt acctggtatg agattgcccg ttttgatcac cgttttgaac gtggactgga
     2101 aaaagtcacc gcaacataca gtctgcgtga tgacggcggc ctgaatgtca tcaataaagg
     2161 ctataaccct gacagagaaa tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc
     2221 accaacacgc gctgcgctga aagtgtcatt ctttggtcct ttctatggcg gttataacgt
     2281 cattgcactt gatcgggaat accgccatgc gctggtttgc ggcccggacc gcgactacct
     2341 gtggatactc tcccgcacgc caaccatttc tgacgaagtg aaacaggaga tgctggcagt
     2401 cgcgacccgg gaagggtttg atgtcagtaa gtttatttgg gtacagcagc ccggtagtta
     2461 gtgagtgctg agtttcagac caataatccc caatacgatt aacgccagac tcgccaggcg
     2521 catcgggtta gcggactcac cgagcagtac aatgccggtg atagccgcgc cgactgcgcc
     2581 aatacccgtc cacacggcat aagccgtccc tactggtaac gatttcatcg cccaggcaag
     2641 tagcgccata ctgacaatca tcgccgtcac ggtaataaca ctcggcgtca aacgactaaa
     2701 gccgtgggta tatttcaggc caacggccca taccacttcc agcagaccag caataactaa
     2761 gataatccag gacatatcag gctccggaga attggggccg tccccgg
//
LOCUS       DAFNBJ010000460         2494 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.303, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000460 DAFNBJ010000000
VERSION     DAFNBJ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.303"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            73..627
                     /locus_tag="L2X49_005157"
     CDS             73..627
                     /locus_tag="L2X49_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ib'"
                     /protein_id="HBN4800066.1"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLA
                     DVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGWWEEETDPGVRGIDQS
                     LANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTV
                     TTPDGPAVYMVQTRQAFERTRSVA"
     gene            697..1485
                     /gene="aadA1"
                     /locus_tag="L2X49_005158"
     CDS             697..1485
                     /gene="aadA1"
                     /locus_tag="L2X49_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBN4800067.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1545..2369
                     /locus_tag="L2X49_005159"
     CDS             1545..2369
                     /locus_tag="L2X49_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-9"
                     /protein_id="HBN4800068.1"
                     /translation="MKKILLLHMLVFVSATLPISSVASDEVETLKCTIIADAITGNTL
                     YETGECARRVSPCSSFKLPLAIMGFDSGILQSPKSPTWELKPEYNPSPRDRTYKQVYP
                     ALWQSDSVVWFSQQLTSRLGVDRFTEYVKKFEYGNQDVSGDSGKHNGLTQSWLMSSLT
                     ISPKEQIQFLLRFVAHKLPVSEAAYDMAYATIPQYQAAEGWAVHGKSGSGWLRDNNGK
                     INESRPQGWFVGWAEKNGRQVVFARLEIGKEKSDIPGGSKAREDILVELPVLMGNK"
BASE COUNT          651 a          611 c          678 g          554 t
ORIGIN      
        1 taatattgaa aaaggaagag tatgagtatt caacatttcc aaacaaagtt aggcatcaca
       61 aagtacagca tcgtgaccaa cagcaacgat tccgtcacac tgcgcctcat gactgagcat
      121 gaccttgcga tgctctatga gtggctaaat cgatctcata tcgtcgagtg gtggggcgga
      181 gaagaagcac gcccgacact tgctgacgta caggaacagt acttgccaag cgttttagcg
      241 caagagtccg tcactccata cattgcaatg ctgaatggag agccgattgg gtatgcccag
      301 tcgtacgttg ctcttggaag cggggacgga tggtgggaag aagaaaccga tccaggagta
      361 cgcggaatag accagtcact ggcgaatgca tcacaactgg gcaaaggctt gggaaccaag
      421 ctggttcgag ctctggttga gttgctgttc aatgatcccg aggtcaccaa gatccaaacg
      481 gacccgtcgc cgagcaactt gcgagcgatc cgatgctacg agaaagcggg gtttgagagg
      541 caaggtaccg taaccacccc agatggtcca gccgtgtaca tggttcaaac acgccaggca
      601 ttcgagcgaa cacgcagtgt tgcctaaccc ttccatcgag ggggacgtcc aagggctggc
      661 gcccttggcc gcccctcatg tcaaacgtta aacatcatga gggaagcggt gatcgccgaa
      721 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
      781 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
      841 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
      901 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
      961 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     1021 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     1081 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     1141 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     1201 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     1261 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     1321 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctagg
     1381 caggcttatc ttggacaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     1441 gttcactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct aaaacaaagt
     1501 tatgcaccta ttaagcgcac agcggagcaa tgaaggatac cttgatgaaa aaaattttgc
     1561 tgctgcatat gttggtgttc gtttccgcca ctctcccaat cagttccgtg gcttctgatg
     1621 aggttgaaac gcttaaatgc accatcatcg cagacgccat taccggaaat accttatatg
     1681 agaccggaga atgtgcccgt cgtgtgtctc cgtgctcgtc ttttaaactt ccattggcaa
     1741 tcatggggtt tgatagtgga atcttgcagt cgccaaaatc acctacgtgg gaattgaagc
     1801 cggaatacaa cccgtctccg agagatcgca catacaaaca agtctatccg gcgctatggc
     1861 aaagcgactc tgttgtctgg ttctcgcagc aattaacaag ccgtctggga gttgatcggt
     1921 tcacggaata cgtaaagaaa tttgagtacg gtaatcaaga tgtttccggt gactcgggga
     1981 agcataacgg cttgacccag tcatggctga tgtcgtcgct caccatatct cccaaggagc
     2041 aaattcagtt tcttctacgc tttgtcgcgc ataagctgcc tgtatccgaa gcggcttatg
     2101 acatggcgta tgccacaatc ccgcagtacc aggcagccga aggatgggct gtacatggaa
     2161 aaagcggcag cggctggctt cgggacaata acggcaagat aaatgaaagt cgtccgcagg
     2221 gctggttcgt gggctgggct gaaaaaaacg gacggcaagt tgttttcgcc cgattggaaa
     2281 taggaaagga aaagtccgat attcccggcg ggtctaaagc acgagaggat attctcgtgg
     2341 aattacccgt gttgatgggt aacaaatgat atgtggcgtc atcgagagca gatgcataac
     2401 cctgcgctcg agcggacctc gcgcataaag ccgcgcgagt ccgctcacct tgaacgttag
     2461 atgcactaag cacataattg ctcacagcca aact
//
LOCUS       DAFNBJ010000461         2369 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.310, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000461 DAFNBJ010000000
VERSION     DAFNBJ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.310"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            110..1015
                     /locus_tag="L2X49_005160"
     CDS             110..1015
                     /locus_tag="L2X49_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBN4800069.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /locus_tag="L2X49_005161"
     CDS             1012..2250
                     /locus_tag="L2X49_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4800070.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..>2369
                     /locus_tag="L2X49_005162"
     CDS             2250..>2369
                     /locus_tag="L2X49_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN4800071.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLS"
BASE COUNT          357 a          761 c          765 g          486 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctcc
//
LOCUS       DAFNBJ010000462         2369 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.311, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000462 DAFNBJ010000000
VERSION     DAFNBJ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.311"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            110..1015
                     /locus_tag="L2X49_005163"
     CDS             110..1015
                     /locus_tag="L2X49_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBN4800072.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEAGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /locus_tag="L2X49_005164"
     CDS             1012..2250
                     /locus_tag="L2X49_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN4800073.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..>2369
                     /locus_tag="L2X49_005165"
     CDS             2250..>2369
                     /locus_tag="L2X49_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN4800074.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLS"
BASE COUNT          356 a          762 c          765 g          486 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagcggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctcc
//
LOCUS       DAFNBJ010000463         2330 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.315, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000463 DAFNBJ010000000
VERSION     DAFNBJ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.315"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            207..1019
                     /gene="blaNDM"
                     /locus_tag="L2X49_005166"
     CDS             207..1019
                     /gene="blaNDM"
                     /locus_tag="L2X49_005166"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDM family subclass B1 metallo-beta-lactamase"
                     /protein_id="HBN4800075.1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQ
                     RFGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQIL
                     NWIKQEINLPVALAVVTHAHQDKMGGMNAMHAAGIATYANALSNQLAPQEGLVAAQHS
                     LTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSL
                     GNLGDADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
     gene            1023..1388
                     /gene="ble"
                     /locus_tag="L2X49_005167"
     CDS             1023..1388
                     /gene="ble"
                     /locus_tag="L2X49_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin binding protein Ble-MBL"
                     /protein_id="HBN4800076.1"
                     /translation="MADHVTPNLPSRDFDVTEAFYAKLGFATSWKDRGWMILQRGGLQ
                     LEFFPYPDLDPATSSFGCCLRLDDLDAMVALVNAAGAEEKSTGWPRFKAPQLEASGLR
                     IGYLIDPDCTLVRLIQNPD"
     gene            1393..2031
                     /locus_tag="L2X49_005168"
     CDS             1393..2031
                     /locus_tag="L2X49_005168"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HBN4800077.1"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVA
                     GALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKP
                     VWKALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALP
                     WGLAGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
     gene            complement(2042..>2330)
                     /locus_tag="L2X49_005169"
     CDS             complement(2042..>2330)
                     /locus_tag="L2X49_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800078.1"
                     /translation="GWFAKYDHPLWKRYADLAAGAGHGGMDWFVIHAFVEALKAKAPM
                     PIDIYDALAWSAITPLSEQSIAEGNRTLDFPDFTRGQWRTRKPIFALNDAY"
BASE COUNT          446 a          700 c          711 g          473 t
ORIGIN      
        1 acaaacatga tctcatatca gggacttgtt cgcaccttcc ttagagaaat ttgctcagct
       61 tgttgattat catatggctt ttgaaactgt cgcacctcat gtttgaattc gccccatatt
      121 tttgctacag tgaaccaaat taagatcatc tatttactag gcctcgcatt tgcggggttt
      181 ttaatgctga ataaaaggaa aacttgatgg aattgcccaa tattatgcac ccggtcgcga
      241 agctgagcac cgcattagcc gctgcattga tgctgagcgg gtgcatgccc ggtgaaatcc
      301 gcccgacgat tggccagcaa atggaaactg gcgaccaacg gtttggcgat ctggttttcc
      361 gccagctcgc accgaatgtc tggcagcaca cttcctatct cgacatgccg ggtttcgggg
      421 cagtcgcttc caacggtttg atcgtcaggg atggcggccg cgtgctggtg gtcgataccg
      481 cctggaccga tgaccagacc gcccagatcc tcaactggat caagcaggag atcaacctgc
      541 cggtcgcgct ggcggtggtg actcacgcgc atcaggacaa gatgggcggt atgaacgcga
      601 tgcatgcggc ggggattgcg acttatgcca atgcgttgtc gaaccagctt gccccgcaag
      661 aggggctggt tgcggcgcaa cacagcctga ctttcgccgc caatggctgg gtcgaaccag
      721 caaccgcgcc caactttggc ccgctcaagg tattttaccc cggccccggc cacaccagtg
      781 acaatatcac cgttgggatc gacggcaccg acatcgcttt tggtggctgc ctgatcaagg
      841 acagcaaggc caagtcgctc ggcaatctcg gtgatgccga cactgagcac tacgccgcgt
      901 cagcgcgcgc gtttggtgcg gcgttcccca aggccagcat gatcgtgatg agccattccg
      961 cccccgatag ccgcgccgca atcactcata cggcccgcat ggccgacaag ctgcgctgag
     1021 ccatggctga ccacgtcacc cccaatctgc catcgcgcga tttcgatgtg acagaggcgt
     1081 tttatgcgaa gctgggcttt gcgacgagtt ggaaggatcg cggctggatg atcctgcagc
     1141 gcggcggttt gcagctcgaa ttcttcccct atcctgacct cgacccagct acgagctcgt
     1201 tcggctgttg cctgcggttg gatgatctcg atgccatggt ggcattggtg aacgcggcgg
     1261 gagccgagga aaaaagcacc ggctggccgc gcttcaaagc tccgcaactg gaggcgagcg
     1321 gcctgaggat cggctacctg atcgatcccg actgcacgct ggtgcggctg atccagaacc
     1381 ccgactgacc gcatgcccgc gaaaatcaag atttgcggga tcagcacacc cgaggcgctc
     1441 gatgcgacca tcgcggcgcg ggcggactat gccgggttgg tgttctatcc agcgtcgccc
     1501 cgtgcggtta cgtcgaatgt cgcgggcgct ttgacatcgc gcgcagctgg ccagatcgcc
     1561 atggtcggtt tgttcgtcga tgcggatgat gctgtcatcg ccgacgcact ggtggcagcc
     1621 aagctgaacg cgctgcagct gcacggttcg gaatcgcccg aacgcgtggc ccagttgcgc
     1681 gcgcggtttg gcaagccggt gtggaaggcg ctgcccgtcg ccagcgccag cgatgtcgca
     1741 cgcgccgcag cctatgccgg ggcggcggac ttgatcttgt tcgacgccaa gacccccaaa
     1801 ggcgcgctgc ccggcggcat ggggttggcg ttcgactggt cgctgctggc cggatatcgc
     1861 ggtgccttgc cgtgggggct ggcaggcggg ctaaatccga cgaatgttgc cgaggcgatt
     1921 gcgcgcaccg gagcgccgct ggtcgatacc tccagcggcg tcgaaagcgc gccgggcgtc
     1981 aaggataccg acaagattac caatttcgcc tttgcggtgc gcttggccta aatcgcgtcg
     2041 atcaataggc gtcgttcagc gcaaagatcg gcttgcgggt gcgccactgc cctcgggtga
     2101 agtcgggaaa atctaacgtg cgattgccct cagcaatcga ttgttccgac agaggcgtga
     2161 tcgcgctcca ggccagcgcg tcgtaaatgt cgattggcat cggggccttg gccttcagcg
     2221 cctcgacaaa agcgtggatc acgaaccagt ccatcccgcc atgcccggcc cctgccgcca
     2281 gatcggcgta gcgtttccat agcgggtgat cgtatttcgc aaaccagccc
//
LOCUS       DAFNBJ010000464         2330 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.316, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000464 DAFNBJ010000000
VERSION     DAFNBJ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.316"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            207..1019
                     /locus_tag="L2X49_005170"
     CDS             207..1019
                     /locus_tag="L2X49_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase NDM-7"
                     /protein_id="HBN4800079.1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQ
                     RFGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQIL
                     NWIKQEINLPVALAVVTHAHQDKMGGMNALHAAGIATYANALSNQLAPQEGLVAAQHS
                     LTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSL
                     GNLGDADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
     gene            1023..1388
                     /gene="ble"
                     /locus_tag="L2X49_005171"
     CDS             1023..1388
                     /gene="ble"
                     /locus_tag="L2X49_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin binding protein Ble-MBL"
                     /protein_id="HBN4800080.1"
                     /translation="MADHVTPNLPSRDFDVTEAFYAKLGFATSWKDRGWMILQRGGLQ
                     LEFFPYPDLDPATSSFGCCLRLDDLDAMVALVNAAGAEEKSTGWPRFKAPQLEASGLR
                     IGYLIDPDCTLVRLIQNPD"
     gene            1393..2031
                     /locus_tag="L2X49_005172"
     CDS             1393..2031
                     /locus_tag="L2X49_005172"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HBN4800081.1"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVA
                     GALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKP
                     VWKALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALP
                     WGLAGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
     gene            complement(2042..>2330)
                     /locus_tag="L2X49_005173"
     CDS             complement(2042..>2330)
                     /locus_tag="L2X49_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800082.1"
                     /translation="GWFAKYDHPLWKRYADLAAGAGHGGMDWFVIHAFVEALKAKAPM
                     PIDIYDALAWSAITPLSEQSIAEGNRTLDFPDFTRGQWRTRKPIFALNDAY"
BASE COUNT          445 a          701 c          711 g          473 t
ORIGIN      
        1 acaaacatga tctcatatca gggacttgtt cgcaccttcc ttagagaaat ttgctcagct
       61 tgttgattat catatggctt ttgaaactgt cgcacctcat gtttgaattc gccccatatt
      121 tttgctacag tgaaccaaat taagatcatc tatttactag gcctcgcatt tgcggggttt
      181 ttaatgctga ataaaaggaa aacttgatgg aattgcccaa tattatgcac ccggtcgcga
      241 agctgagcac cgcattagcc gctgcattga tgctgagcgg gtgcatgccc ggtgaaatcc
      301 gcccgacgat tggccagcaa atggaaactg gcgaccaacg gtttggcgat ctggttttcc
      361 gccagctcgc accgaatgtc tggcagcaca cttcctatct cgacatgccg ggtttcgggg
      421 cagtcgcttc caacggtttg atcgtcaggg atggcggccg cgtgctggtg gtcgataccg
      481 cctggaccga tgaccagacc gcccagatcc tcaactggat caagcaggag atcaacctgc
      541 cggtcgcgct ggcggtggtg actcacgcgc atcaggacaa gatgggcggt atgaacgcgc
      601 tgcatgcggc ggggattgcg acttatgcca atgcgttgtc gaaccagctt gccccgcaag
      661 aggggctggt tgcggcgcaa cacagcctga ctttcgccgc caatggctgg gtcgaaccag
      721 caaccgcgcc caactttggc ccgctcaagg tattttaccc cggccccggc cacaccagtg
      781 acaatatcac cgttgggatc gacggcaccg acatcgcttt tggtggctgc ctgatcaagg
      841 acagcaaggc caagtcgctc ggcaatctcg gtgatgccga cactgagcac tacgccgcgt
      901 cagcgcgcgc gtttggtgcg gcgttcccca aggccagcat gatcgtgatg agccattccg
      961 cccccgatag ccgcgccgca atcactcata cggcccgcat ggccgacaag ctgcgctgag
     1021 ccatggctga ccacgtcacc cccaatctgc catcgcgcga tttcgatgtg acagaggcgt
     1081 tttatgcgaa gctgggcttt gcgacgagtt ggaaggatcg cggctggatg atcctgcagc
     1141 gcggcggttt gcagctcgaa ttcttcccct atcctgacct cgacccagct acgagctcgt
     1201 tcggctgttg cctgcggttg gatgatctcg atgccatggt ggcattggtg aacgcggcgg
     1261 gagccgagga aaaaagcacc ggctggccgc gcttcaaagc tccgcaactg gaggcgagcg
     1321 gcctgaggat cggctacctg atcgatcccg actgcacgct ggtgcggctg atccagaacc
     1381 ccgactgacc gcatgcccgc gaaaatcaag atttgcggga tcagcacacc cgaggcgctc
     1441 gatgcgacca tcgcggcgcg ggcggactat gccgggttgg tgttctatcc agcgtcgccc
     1501 cgtgcggtta cgtcgaatgt cgcgggcgct ttgacatcgc gcgcagctgg ccagatcgcc
     1561 atggtcggtt tgttcgtcga tgcggatgat gctgtcatcg ccgacgcact ggtggcagcc
     1621 aagctgaacg cgctgcagct gcacggttcg gaatcgcccg aacgcgtggc ccagttgcgc
     1681 gcgcggtttg gcaagccggt gtggaaggcg ctgcccgtcg ccagcgccag cgatgtcgca
     1741 cgcgccgcag cctatgccgg ggcggcggac ttgatcttgt tcgacgccaa gacccccaaa
     1801 ggcgcgctgc ccggcggcat ggggttggcg ttcgactggt cgctgctggc cggatatcgc
     1861 ggtgccttgc cgtgggggct ggcaggcggg ctaaatccga cgaatgttgc cgaggcgatt
     1921 gcgcgcaccg gagcgccgct ggtcgatacc tccagcggcg tcgaaagcgc gccgggcgtc
     1981 aaggataccg acaagattac caatttcgcc tttgcggtgc gcttggccta aatcgcgtcg
     2041 atcaataggc gtcgttcagc gcaaagatcg gcttgcgggt gcgccactgc cctcgggtga
     2101 agtcgggaaa atctaacgtg cgattgccct cagcaatcga ttgttccgac agaggcgtga
     2161 tcgcgctcca ggccagcgcg tcgtaaatgt cgattggcat cggggccttg gccttcagcg
     2221 cctcgacaaa agcgtggatc acgaaccagt ccatcccgcc atgcccggcc cctgccgcca
     2281 gatcggcgta gcgtttccat agcgggtgat cgtatttcgc aaaccagccc
//
LOCUS       DAFNBJ010000465         1675 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.342, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000465 DAFNBJ010000000
VERSION     DAFNBJ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.342"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            154..1629
                     /gene="msr(E)"
                     /locus_tag="L2X49_005174"
     CDS             154..1629
                     /gene="msr(E)"
                     /locus_tag="L2X49_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(E)"
                     /protein_id="HBN4800083.1"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS
                     SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG
                     GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD
                     MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ
                     QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE
                     HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG
                     KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL
                     ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM
                     MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
BASE COUNT          555 a          292 c          365 g          463 t
ORIGIN      
        1 gtttccagtt ataacttaat tgactagcta tttgtccacc ctgtggatga atagcttttt
       61 ttttgggagg acactgtgat gctagctttt gttttcacct aaatcctgtt tgctgcataa
      121 aaaatttcaa gagctaaaca ggagtaaata aaaatgagtt taattattaa agcgagaaac
      181 atacgcttgg attatgctgg gcgtgatgtt ttggatattg atgaattgga aattcactct
      241 tatgaccgta ttggtcttgt gggtgataac ggagcaggaa agagtagttt actcaaagta
      301 cttaatggcg aaattgtttt agccgaagcg acattacagc gttttggtga ttttgcacat
      361 atcagccaac tgggcggaat cgaaatagaa acggtcgaag accgggcaat gttatctcgc
      421 cttggtgttt ccaatgtaca aaacgacaca atgagtggcg gagaggaaac tcgtgcaaaa
      481 attgctgccg cattttccca acaagtacat ggcattctag cggatgaacc aaccagccac
      541 cttgatctca atggaataga tctacttatt ggtcaactta aagcatttga tggagcatta
      601 cttgttatca gtcatgaccg atattttctt gatatggttg tagacaagat atgggagtta
      661 aaagacggta aaattacgga atattggggt ggttactcgg attacttgcg tcaaaaagaa
      721 gaagagcgac aacaccaagc cgtagaatat gagctgatga tgaaggaacg ggagcgatta
      781 gaatctgctg tgcaagaaaa acgccagcaa gctaatcgat tagacaataa gaaaaaagga
      841 gaaaaatcca aaaactctac cgaaagtgct ggacgacttg ggcatgcaaa aatgactggc
      901 accaagcaaa gaaaactgta tcaggcagct aagagtatgg aaaagcgttt ggctgcatta
      961 gaagatattc aagcaccaga gcatttgcgt tctattcgtt ttcgtcaaag ttcagcccta
     1021 gaactgcaca ataagttccc gattacggca gatggtctga gcttaaaatt tggtagccgt
     1081 actatctttg atgacgctaa ctttataata ccgcttggcg ctaaagtcgc tataactgga
     1141 tcgaatggaa cagggaaaac gtccttgtta aaaatgatat cagaacgtgc tgatggatta
     1201 accatatctc caaaagctga aattggctac tttacacaaa caggatataa atttaacacg
     1261 cataaatctg tgctctcctt tatgcaggaa gagtgcgagt acacagttgc ggaaattcgt
     1321 gcagtattgg cttcaatggg gatcggagcg aatgatattc aaaaaaactt atccgactta
     1381 tcgggaggtg aaatcatcaa actgctttta tccaaaatgc ttttaggaaa atataatatt
     1441 ttgcttatgg atgaaccagg aaactatctt gacctaaaaa gtattgccgc attagaaaca
     1501 atgatgaagt cctatgcagg aactattatc ttcgtatctc atgacaagca attggtcgat
     1561 aatattgctg acattatcta cgagatcaaa gaccacaaaa tcatcaagac ttttgagaga
     1621 gattgttaat gatagccaat ctaatccgaa cattaattat tgaactcttt aaagg
//
LOCUS       DAFNBJ010000466         1415 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.356, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000466 DAFNBJ010000000
VERSION     DAFNBJ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.356"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..360)
                     /locus_tag="L2X49_005175"
     CDS             complement(<1..360)
                     /locus_tag="L2X49_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN4800084.1"
                     /translation="MRSNPPLNALRAFEASARHLSFTRAALELYVTQAAVSQQVRLLE
                     ERLGMVLFKRLPRGLEMTEESRALFAVLTDAFGHIEKAFRQFEGGQFQEVLTVAAVGT
                     FAVGWLLPRLDRFRQEHP"
     gene            493..1380
                     /locus_tag="L2X49_005176"
     CDS             493..1380
                     /locus_tag="L2X49_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase SFO-1"
                     /protein_id="HBN4800085.1"
                     /translation="MVKNTLRQTTLMVATVMPLLFGSAPLWAQSANAKANIQQQLSEL
                     EKNSGGRLGVALIDTADNSQILYRGDERFPMCSTSKVMAVSALLKQSEMDKNLLAKRM
                     EIKQSDLVNYNPIAEKHLDTGMTLAELSAAAIQYSDNTAMNKILEHLGGPAKVTEYAR
                     TIGDKTFRLDRTEPTLNTAIPSDKRDTTSPLAMAKSLQTLTLGKALGEPQRAQLVEWM
                     KGNTTGGASIRAGLPATWVVGDKTGSGDYGTTNDIAVIWPANHAPLVLVTYFTQPQQN
                     AEARKDVLAAAAKIVTEGL"
BASE COUNT          375 a          368 c          368 g          304 t
ORIGIN      
        1 aggatgctct tgcctgaacc gatcgagacg tggtaacagc cagcccacgg caaaagtgcc
       61 gacggcagcc acggtaagca cctcctggaa ctgcccccct tcaaactgcc ggaaggcttt
      121 ttcaatatgg ccaaaggcgt cggttaacac ggcaaacagc gcccgtgatt cctctgtcat
      181 ttctaaacca cggggtaatc gtttaaacag caccatgccc aggcgctctt caagcagccg
      241 aacctgttgg ctaacggcag cctgggtaac atagagctcc agcgctgccc gagtgaaact
      301 caaatgtctg gcagaggctt caaaagcacg tagagcattc aggggagggt tagaacgcat
      361 aggtattatc caaaagattt tctttatgtt agagaaaatt cttgtcgctt gtcaataacg
      421 gtctaaaaga agatagttct cgccatctgc agaacaaccc gctgagttaa tccattttat
      481 gtgaggcaaa acatggttaa aaatacatta cgtcaaacca ccctgatggt cgctacggtt
      541 atgccgctgc tgttcggtag cgcaccatta tgggctcaat ccgctaatgc caaagcgaat
      601 attcagcagc aactgtccga gctcgagaaa aactccggtg gccgccttgg cgtggcgctg
      661 atcgataccg ccgataattc gcagatcctg tatcgtgggg atgaacgttt tcccatgtgt
      721 agcactagca aggtgatggc ggtgtcggcg ttgttaaaac agagcgagat ggataaaaat
      781 cttttggcta agcggatgga aatcaaacaa tccgatctgg tcaactacaa cccgatcgcc
      841 gaaaaacacc tggataccgg gatgaccctt gcagagctca gtgccgccgc catccagtac
      901 agtgacaaca cggcgatgaa caagatcctt gagcatcttg gcggcccggc aaaagtgaca
      961 gagtatgcgc gtactattgg tgataaaacc tttcgtctcg atcgtaccga gcctacttta
     1021 aatactgcca ttcccagcga taagcgtgac actacctcgc cgctggcgat ggcaaaaagc
     1081 ctgcaaaccc taactttggg caaggcgctg ggtgaaccac agcgtgctca actggttgaa
     1141 tggatgaagg ggaacactac cggcggagcc agcattcgcg caggtctgcc agccacgtgg
     1201 gtggtcggtg ataaaaccgg cagtggtgat tacggtacca ctaacgatat cgccgtgatt
     1261 tggccagcga accacgcacc gttggtgttg gtaacctatt tcactcagcc acagcagaat
     1321 gcagaagccc gcaaagacgt gttggctgct gccgctaaaa ttgtcaccga agggctttga
     1381 atcagggata tgcaccgcgt atctgcgccg ggatt
//
LOCUS       DAFNBJ010000467         1209 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.366, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000467 DAFNBJ010000000
VERSION     DAFNBJ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.366"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            61..816
                     /locus_tag="L2X49_005177"
     CDS             61..816
                     /locus_tag="L2X49_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1405)-N(7))-methyltransferase
                     RmtB1"
                     /protein_id="HBN4800086.1"
                     /translation="MNINDALTSILASKKYRALCPDTVRRILTEEWGRHKSPKQTVEA
                     ARTRLHGICGAYVTPESLKAAAAALSAGDVKKALSLHASTKERLAELDTLYDFIFSAE
                     TPRRVLDIACGLNPLALYERGIASVWGCDIHQGLGDVITPFAREKDWDFTFALQDVLC
                     APPAEAGDLALIFKLLPLLEREQAGSAMALLQSLNTPRMAVSFPTRSLGGRGKGMEAN
                     YAAWFEGGLPAEFEIEDKKTIGTELIYLIKKNG"
     gene            complement(897..1178)
                     /locus_tag="L2X49_005178"
     CDS             complement(897..1178)
                     /locus_tag="L2X49_005178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN4800087.1"
                     /translation="MLLITAALLFRMLGVWMSTLGTDLSRKERLFCMIAYLPKATVQA
                     AIGAIPLAMGLGSGETILAVAVLAIILTAPLGALGIELSYKRLLQKQQS"
BASE COUNT          285 a          347 c          321 g          256 t
ORIGIN      
        1 cttaacgtga gttttcgttc cactgagcgt cagaccccga aaacgattta ggagacaccg
       61 atgaacatca acgatgccct cacctccatc ctggcctcaa aaaaataccg cgccctttgc
      121 ccggataccg tgcggcgcat cctgactgag gaatgggggc ggcataaatc ccccaaacag
      181 accgtagagg ctgcacgcac ccggctgcat ggaatttgcg gggcatatgt caccccggaa
      241 tcgctcaagg ctgctgccgc cgcgctttct gcgggcgatg taaaaaaggc attgtcgctg
      301 catgcctcca ccaaggagcg actggccgag ctggataccc tgtacgattt tatcttttca
      361 gccgaaactc cccgccgcgt gctggatatc gcctgcggtc ttaacccctt ggcgctatac
      421 gagcgcggca ttgcatccgt gtggggctgt gatatccacc agggattggg ggatgtcatc
      481 accccctttg ctagggaaaa agattgggat tttacctttg ccctgcagga tgtgctgtgt
      541 gcgccgcccg ccgaagccgg cgacctggcg ctgattttta agcttttgcc cctgctggag
      601 cgggagcagg ccggttctgc catggcactt ttacaatccc tcaatacccc gcgcatggct
      661 gtcagctttc ccacgcgtag tttaggcggg cgtggaaaag gcatggaggc gaactacgcc
      721 gcatggttcg agggcggctt gcccgccgag tttgagattg aggataaaaa gaccatcgga
      781 acagaactta tatacttgat aaaaaagaat ggataagcca atcagaaggc aaatcatcta
      841 aaaaagagga acgaaaaata tttttcgttc ctcttttgtt ctatcgaaca agcacactac
      901 gactgctgtt tttgaagcag ccttttatag ctcagctcga tgcccaacgc ccccagcggc
      961 gctgtaagta taatggcaag aaccgccacc gcaagtatgg tttccccact gccaagcccc
     1021 atcgccagcg gaatagcgcc gattgcagcc tgcaccgttg ccttgggcag ataggcaatc
     1081 atgcagaaca ggcgctcctt ccgggatagg tctgtaccca gcgtactcat ccacacgccc
     1141 agcatgcgaa acagcagcgc agctgtgatc agcagcatgc cgcttagccc ggcggagaaa
     1201 aggtaacgg
//
LOCUS       DAFNBJ010000468         1051 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.384, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000468 DAFNBJ010000000
VERSION     DAFNBJ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.384"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            89..964
                     /locus_tag="L2X49_005179"
     CDS             89..964
                     /locus_tag="L2X49_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-15"
                     /protein_id="HBN4800088.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
BASE COUNT          267 a          259 c          296 g          229 t
ORIGIN      
        1 tgatttttga agctaataaa aaacacacgt ggaatttagg gactattcat gttgttgtta
       61 tttcgtatct tccagaataa ggaatcccat ggttaaaaaa tcactgcgcc agttcacgct
      121 gatggcgacg gcaaccgtca cgctgttgtt aggaagtgtg ccgctgtatg cgcaaacggc
      181 ggacgtacag caaaaacttg ccgaattaga gcggcagtcg ggaggcagac tgggtgtggc
      241 attgattaac acagcagata attcgcaaat actttatcgt gctgatgagc gctttgcgat
      301 gtgcagcacc agtaaagtga tggccgcggc cgcggtgctg aagaaaagtg aaagcgaacc
      361 gaatctgtta aatcagcgag ttgagatcaa aaaatctgac cttgttaact ataatccgat
      421 tgcggaaaag cacgtcaatg ggacgatgtc actggctgag cttagcgcgg ccgcgctaca
      481 gtacagcgat aacgtggcga tgaataagct gattgctcac gttggcggcc cggctagcgt
      541 caccgcgttc gcccgacagc tgggagacga aacgttccgt ctcgaccgta ccgagccgac
      601 gttaaacacc gccattccgg gcgatccgcg tgataccact tcacctcggg caatggcgca
      661 aactctgcgg aatctgacgc tgggtaaagc attgggcgac agccaacggg cgcagctggt
      721 gacatggatg aaaggcaata ccaccggtgc agcgagcatt caggctggac tgcctgcttc
      781 ctgggttgtg ggggataaaa ccggcagcgg tggctatggc accaccaacg atatcgcggt
      841 gatctggcca aaagatcgtg cgccgctgat tctggtcact tacttcaccc agcctcaacc
      901 taaggcagaa agccgtcgcg atgtattagc gtcggcggct aaaatcgtca ccgacggttt
      961 gtaatagcgg aaacggaatg gggaaactca ttccgttttt gtttatcgcc ttagacggca
     1021 aaagtgctgt cgcccacctg cgcttgcgca t
//
LOCUS       DAFNBJ010000469          948 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.392, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000469 DAFNBJ010000000
VERSION     DAFNBJ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.392"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            complement(<1..191)
                     /locus_tag="L2X49_005180"
     CDS             complement(<1..191)
                     /locus_tag="L2X49_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBN4800089.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAG"
     gene            452..910
                     /gene="gpt"
                     /locus_tag="L2X49_005181"
     CDS             452..910
                     /gene="gpt"
                     /locus_tag="L2X49_005181"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBN4800090.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDNYVVDIPQDTWIEQPWDMGVVFVPPISGR"
BASE COUNT          240 a          224 c          239 g          245 t
ORIGIN      
        1 cctgcggtag caggtttacg aatcaggata ttacctacct gatcgcgttc gacatggaaa
       61 cctttctctt ttgcccaacc aacaatgtat tcagcgagtt gctcttcatg ataggacggg
      121 tgaggaatag aacagatttt ggcaaaaata tcccacagcg gctgtggaga taattgagac
      181 agttcagaca cgttaagtct ccttgtcgat cacccgcaaa acagtattgc aggtcacagg
      241 gttagcagaa aatgttgtca acacaagaca ggcttgcgag atatgtttga gaataccact
      301 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
      361 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
      421 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
      481 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
      541 aggcattatt gccgtaagcc gtggcggtct ggtaccgggt gcgttactgg cgcgtgaact
      601 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
      661 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
      721 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
      781 caccatcttt gcaaaaccgg ctggtcgtcc gctggttgat aattatgttg ttgatatccc
      841 gcaagatacc tggatcgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
      901 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttctttt
//
LOCUS       DAFNBJ010000470          926 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.395, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000470 DAFNBJ010000000
VERSION     DAFNBJ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.395"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            22..882
                     /gene="blaTEM"
                     /locus_tag="L2X49_005182"
     CDS             22..882
                     /gene="blaTEM"
                     /locus_tag="L2X49_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TEM family class A beta-lactamase"
                     /protein_id="HBN4800091.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWREADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          249 a          210 c          232 g          235 t
ORIGIN      
        1 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
       61 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      121 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      181 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      241 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
      301 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      361 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgctgcc
      421 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
      481 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
      541 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
      601 ggcgaactac ttactctagc ttcccggcaa caattaatag actggaggga ggcggataaa
      661 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
      721 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
      781 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
      841 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
      901 tcatatatac tttagattga tttaaa
//
LOCUS       DAFNBJ010000471          926 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.396, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000471 DAFNBJ010000000
VERSION     DAFNBJ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.396"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            22..882
                     /locus_tag="L2X49_005183"
     CDS             22..882
                     /locus_tag="L2X49_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBN4800092.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          249 a          210 c          231 g          236 t
ORIGIN      
        1 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
       61 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      121 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      181 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      241 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
      301 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      361 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgctgcc
      421 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
      481 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
      541 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
      601 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
      661 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
      721 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
      781 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
      841 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
      901 tcatatatac tttagattga tttaaa
//
LOCUS       DAFNBJ010000472          905 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.398, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000472 DAFNBJ010000000
VERSION     DAFNBJ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.398"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..861
                     /gene="blaTEM"
                     /locus_tag="L2X49_005184"
     CDS             1..861
                     /gene="blaTEM"
                     /locus_tag="L2X49_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TEM family class A beta-lactamase"
                     /protein_id="HBN4800093.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWREADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          238 a          209 c          227 g          231 t
ORIGIN      
        1 atgagtattc aacattttcg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgctgcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggagggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaa
//
LOCUS       DAFNBJ010000473          905 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.399, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000473 DAFNBJ010000000
VERSION     DAFNBJ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.399"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..861
                     /locus_tag="L2X49_005185"
     CDS             1..861
                     /locus_tag="L2X49_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBN4800094.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          238 a          209 c          226 g          232 t
ORIGIN      
        1 atgagtattc aacattttcg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgctgcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaa
//
LOCUS       DAFNBJ010000474          905 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.400, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000474 DAFNBJ010000000
VERSION     DAFNBJ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.400"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..861
                     /gene="blaTEM"
                     /locus_tag="L2X49_005186"
     CDS             1..861
                     /gene="blaTEM"
                     /locus_tag="L2X49_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TEM family class A beta-lactamase"
                     /protein_id="HBN4800095.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWREADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          238 a          208 c          227 g          232 t
ORIGIN      
        1 atgagtattc aacattttcg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggtgc ggtattatcc
      241 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgctgcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggagggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaa
//
LOCUS       DAFNBJ010000475          905 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.401, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000475 DAFNBJ010000000
VERSION     DAFNBJ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.401"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            1..861
                     /locus_tag="L2X49_005187"
     CDS             1..861
                     /locus_tag="L2X49_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBN4800096.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          238 a          208 c          226 g          233 t
ORIGIN      
        1 atgagtattc aacattttcg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggtgc ggtattatcc
      241 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgctgcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaa
//
LOCUS       DAFNBJ010000476          854 bp    DNA     linear   BCT 19-JAN-2022
DEFINITION  TPA_asm: Escherichia coli strain IOMTU783
            SAMD00062425-rid13761383.guided.406, whole genome shotgun sequence.
ACCESSION   DAFNBJ010000476 DAFNBJ010000000
VERSION     DAFNBJ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00062425
            Sequence Read Archive: DRR075645
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001226677.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001226677.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/19/2022 23:52:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,104
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 56 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 37 of 162
            Pseudo Genes (multiple problems)  :: 20 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00062425-rid13761383.guided.406"
                     /strain="IOMTU783"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Nepal: Kathmandu"
                     /lat_lon="27.7 N 85.3 E"
                     /collection_date="2013"
     gene            <1..825
                     /locus_tag="L2X49_005188"
     CDS             <1..825
                     /locus_tag="L2X49_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="HBN4800097.1"
                     /translation="MNFNEMGIDFKVVFALDTKGQQWLLRIPRRDGMREQIKKEKRIL
                     ELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAETYEIIWNMDKDSPKYITSLA
                     KTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLVKSEIGISEQLETRYRKWLDN
                     DVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAHIDDPAIDFAGHVTLFGEESL
                     KTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFALETQNESLIVGAKAQLGVI"
BASE COUNT          291 a          127 c          181 g          255 t
ORIGIN      
        1 atgaatttca atgaaatggg cattgatttt aaggtcgttt ttgctcttga tacaaagggg
       61 caacaatggt tgctgcgtat tcctcgtcgt gatggcatga gggaacaaat caagaaagaa
      121 aaacgcattt tagaattggt aaaaaaacat ctttctgtag aggttcctga ttggagaatt
      181 tcatctacag aattagtggc ttatcccata cttaaagata atcctgtttt aaatttggat
      241 gctgaaacct atgaaataat ttggaatatg gacaaagata gcccgaaata cataacatct
      301 ttggcaaaaa ccttatttga aatccatagt attcctgaaa aagaagttcg ggaaaatgat
      361 ttgaaaatta tgaaaccttc agatttaaga cctgaaatag caaacaattt gcagttagta
      421 aaatctgaaa ttggtataag tgagcaattg gaaacccgct acagaaaatg gttggataat
      481 gatgttctat gggcagattt cacccaattt atacatggcg atttatatgc tgggcatgta
      541 ctagcttcaa aggatggagc tgtttcaggc gttattgatt ggtcaacagc ccatatagat
      601 gacccagcga ttgattttgc tgggcatgta actttgtttg gagaagaaag cctcaaaact
      661 ctaatcatcg agtatgaaaa actagggggt aaagtttgga ataaactata tgaacagact
      721 ttagaaagag cagcggcctc tcctttgatg tatggtttat ttgccttaga aactcaaaat
      781 gaaagcctta tcgttggagc aaaagctcag ttgggagtta tataatttaa aaatatgatt
      841 gctgagaact gcct
//
